LOCUS       DAPHCV010000001        89791 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.001, whole genome shotgun sequence.
ACCESSION   DAPHCV010000001 DAPHCV010000000
VERSION     DAPHCV010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 89791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.001"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..205)
                     /locus_tag="RH033_000001"
     CDS             complement(<1..205)
                     /locus_tag="RH033_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDV2989249.1"
                     /translation="MELNCPICQNSLERNGETAHCATCDKDFTVQAICPECHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEF"
     gene            complement(267..1739)
                     /gene="der"
                     /locus_tag="RH033_000002"
     CDS             complement(267..1739)
                     /gene="der"
                     /locus_tag="RH033_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDV2989250.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     TPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKSK"
     gene            complement(1857..3035)
                     /gene="bamB"
                     /locus_tag="RH033_000003"
     CDS             complement(1857..3035)
                     /gene="bamB"
                     /locus_tag="RH033_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HDV2989251.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVNLAEKDG
                     WFSKDPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(3046..3666)
                     /gene="yfgM"
                     /locus_tag="RH033_000004"
     CDS             complement(3046..3666)
                     /gene="yfgM"
                     /locus_tag="RH033_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HDV2989252.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDAIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(3684..4958)
                     /gene="hisS"
                     /locus_tag="RH033_000005"
     CDS             complement(3684..4958)
                     /gene="hisS"
                     /locus_tag="RH033_000005"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDV2989253.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(5069..6187)
                     /gene="ispG"
                     /locus_tag="RH033_000006"
                     /gene_synonym="gcpE"
     CDS             complement(5069..6187)
                     /gene="ispG"
                     /locus_tag="RH033_000006"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDV2989254.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(6214..7221)
                     /gene="rodZ"
                     /locus_tag="RH033_000007"
     CDS             complement(6214..7221)
                     /gene="rodZ"
                     /locus_tag="RH033_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HDV2989255.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELNNNQSQ
                     SVPLDTSTTTDQAMATTPTSPVDTTATNTQTPAVTAPAPAVDPQQNAVVPPSQANVDT
                     AATPAPAATTTPDGAAPLPTDQAGVTTPAVDPNALVMNFTADCWLEVTDATGKKLFSG
                     MQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQSPA
                     Q"
     gene            complement(7506..8660)
                     /gene="trmG/rlmN"
                     /locus_tag="RH033_000008"
     CDS             complement(7506..8660)
                     /gene="trmG/rlmN"
                     /locus_tag="RH033_000008"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HDV2989256.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(8810..9241)
                     /gene="ndk"
                     /locus_tag="RH033_000009"
     CDS             complement(8810..9241)
                     /gene="ndk"
                     /locus_tag="RH033_000009"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDV2989257.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGSIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(9390..11702)
                     /gene="pbpC"
                     /locus_tag="RH033_000010"
     CDS             complement(9390..11702)
                     /gene="pbpC"
                     /locus_tag="RH033_000010"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HDV2989258.1"
                     /translation="MPRLLTKRGCWIMLAAAPFIIILAAWAADKLWPLPLQEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSRERVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLFERPLMSSGAAWIIRRIMADEAQPLPDGDLPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSVNLPEDPRPYSVSRGVICWPGGQSLPEGDANCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(11703..16664)
                     /gene="yfhM"
                     /locus_tag="RH033_000011"
     CDS             complement(11703..16664)
                     /gene="yfhM"
                     /locus_tag="RH033_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HDV2989259.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     VSSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQEFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDDEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVRFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     YEGWQSQFEQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPTKMRPNQPLTVKIKASNKNGEMPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGDIQATISGLALPGETVADQHKQWTIGVRPAFPAQTVNYGTALQPGETWAL
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMISIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDAMRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDSALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TLQDLPGKWQAQTTFSAEPLTGEKAQTSNLNSDQLATLQVTNSGDQPLWLRVDASGYP
                     QSAPLPASNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLS
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAADDLLIVRP"
     gene            16871..17716
                     /gene="sseA"
                     /locus_tag="RH033_000012"
     CDS             16871..17716
                     /gene="sseA"
                     /locus_tag="RH033_000012"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HDV2989260.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPEK"
     gene            complement(18535..19311)
                     /gene="sseB"
                     /locus_tag="RH033_000013"
     CDS             complement(18535..19311)
                     /gene="sseB"
                     /locus_tag="RH033_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HDV2989261.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDCTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEVLEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQAAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(19430..20713)
                     /gene="pepB"
                     /locus_tag="RH033_000014"
     CDS             complement(19430..20713)
                     /gene="pepB"
                     /locus_tag="RH033_000014"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HDV2989262.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGTDDLGLI
                     QRAARKIDGLGIKHVQLSGEDWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLRDQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAARLLAS
                     AAQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(20772..20972)
                     /gene="iscX"
                     /locus_tag="RH033_000015"
     CDS             complement(20772..20972)
                     /gene="iscX"
                     /locus_tag="RH033_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HDV2989263.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(20984..21319)
                     /gene="fdx"
                     /locus_tag="RH033_000016"
     CDS             complement(20984..21319)
                     /gene="fdx"
                     /locus_tag="RH033_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HDV2989264.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDVALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(21321..23171)
                     /gene="hscA"
                     /locus_tag="RH033_000017"
     CDS             complement(21321..23171)
                     /gene="hscA"
                     /locus_tag="RH033_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HDV2989265.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREKAGIPDRSDNRVQRELLDTAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSTAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(23188..23703)
                     /gene="hscB"
                     /locus_tag="RH033_000018"
     CDS             complement(23188..23703)
                     /gene="hscB"
                     /locus_tag="RH033_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HDV2989266.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(23799..24122)
                     /gene="iscA"
                     /locus_tag="RH033_000019"
     CDS             complement(23799..24122)
                     /gene="iscA"
                     /locus_tag="RH033_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HDV2989267.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(24139..24525)
                     /gene="iscU"
                     /locus_tag="RH033_000020"
     CDS             complement(24139..24525)
                     /gene="iscU"
                     /locus_tag="RH033_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HDV2989268.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(24553..25767)
                     /gene="iscS"
                     /locus_tag="RH033_000021"
     CDS             complement(24553..25767)
                     /gene="iscS"
                     /locus_tag="RH033_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDV2989269.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(25879..26367)
                     /gene="iscR"
                     /locus_tag="RH033_000022"
     CDS             complement(25879..26367)
                     /gene="iscR"
                     /locus_tag="RH033_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HDV2989270.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(26637..27377)
                     /gene="trmJ"
                     /locus_tag="RH033_000023"
     CDS             complement(26637..27377)
                     /gene="trmJ"
                     /locus_tag="RH033_000023"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HDV2989271.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQAEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            27496..28299
                     /gene="suhB"
                     /locus_tag="RH033_000024"
     CDS             27496..28299
                     /gene="suhB"
                     /locus_tag="RH033_000024"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HDV2989272.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            28444..29298
                     /locus_tag="RH033_000025"
     CDS             28444..29298
                     /locus_tag="RH033_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV2989273.1"
                     /translation="MALPVNKRVLKILFILFVVAFCIYLIPRIAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLHGWFIPSATGPAEKAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            29489..30769
                     /gene="csiE"
                     /locus_tag="RH033_000026"
     CDS             29489..30769
                     /gene="csiE"
                     /locus_tag="RH033_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HDV2989274.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRKSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRSAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(30761..31900)
                     /locus_tag="RH033_000027"
     CDS             complement(30761..31900)
                     /locus_tag="RH033_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HDV2989275.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTAMPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(32060..32950)
                     /gene="hcaR"
                     /locus_tag="RH033_000028"
     CDS             complement(32060..32950)
                     /gene="hcaR"
                     /locus_tag="RH033_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HDV2989276.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTEQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            33086..34447
                     /locus_tag="RH033_000029"
     CDS             33086..34447
                     /locus_tag="RH033_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HDV2989277.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            34444..34962
                     /locus_tag="RH033_000030"
     CDS             34444..34962
                     /locus_tag="RH033_000030"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HDV2989278.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGIQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            34962..35282
                     /locus_tag="RH033_000031"
     CDS             34962..35282
                     /locus_tag="RH033_000031"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HDV2989279.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            35279..36091
                     /gene="hcaB"
                     /locus_tag="RH033_000032"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             35279..36091
                     /gene="hcaB"
                     /locus_tag="RH033_000032"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HDV2989280.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            36101..37303
                     /locus_tag="RH033_000033"
     CDS             36101..37303
                     /locus_tag="RH033_000033"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HDV2989281.1"
                     /translation="MKEKTIIIVGGGQAAAMAAALLRQQGFSGELHLFSDEQHLPYER
                     PPLSKSMLLEDSPQLQSVLPAHWWQENNVHLHSGVTIKTLGRDTQELVLANGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRGCKVTVIELAASVMGRNAPPPVQRYLLPRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLRADVVIYGIGISANDQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRIDNGALHRCESWENANNQAQIAAAAMLGLPLPRLPPPWFWSDQYSDNLQFIGDM
                     HGDDWICRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRLIRKWIQSGKTFDAKLLID
                     EDIALKSL"
     gene            37400..37822
                     /locus_tag="RH033_000034"
     CDS             37400..37822
                     /locus_tag="RH033_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDV2989282.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(37870..38742)
                     /locus_tag="RH033_000035"
     CDS             complement(37870..38742)
                     /locus_tag="RH033_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HDV2989283.1"
                     /translation="MTIYTLSHGPLKLDVSDRGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGQWQCASCSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQAIGYWLEREQWLAGEFCEQLPQELDFNQLAPLPCQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVALL"
     gene            complement(38754..39848)
                     /locus_tag="RH033_000036"
     CDS             complement(38754..39848)
                     /locus_tag="RH033_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDV2989284.1"
                     /translation="MKINRIVNEEFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGEGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFTLEQAGEAYALMASGKCGKVVINFPD"
     gene            complement(39881..40879)
                     /locus_tag="RH033_000037"
     CDS             complement(39881..40879)
                     /locus_tag="RH033_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2989285.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(40904..42415)
                     /locus_tag="RH033_000038"
     CDS             complement(40904..42415)
                     /locus_tag="RH033_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDV2989286.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIMDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(42438..43421)
                     /locus_tag="RH033_000039"
     CDS             complement(42438..43421)
                     /locus_tag="RH033_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDV2989287.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(43518..46799)
                     /locus_tag="RH033_000040"
     CDS             complement(43518..46799)
                     /locus_tag="RH033_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HDV2989288.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLKGYRLAIREIGKCN
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNITLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGHFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESAFTQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVTKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAITLWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAHYESARAELTAINPAWPKAALFTTVMPFI
                     FNRVH"
     gene            46917..48110
                     /locus_tag="RH033_000041"
     CDS             46917..48110
                     /locus_tag="RH033_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDV2989289.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQGRDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(48173..49426)
                     /gene="glyA"
                     /locus_tag="RH033_000042"
     CDS             complement(48173..49426)
                     /gene="glyA"
                     /locus_tag="RH033_000042"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDV2989290.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDASGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            49754..50944
                     /gene="hmpA"
                     /locus_tag="RH033_000043"
     CDS             49754..50944
                     /gene="hmpA"
                     /locus_tag="RH033_000043"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HDV2989291.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMDVTDDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWLHAAENGDVHAFADEVKELGLSLPQFTAHTWYRQPSEADRVK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(50989..51327)
                     /gene="glnB"
                     /locus_tag="RH033_000044"
     CDS             complement(50989..51327)
                     /gene="glnB"
                     /locus_tag="RH033_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HDV2989292.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(51388..52722)
                     /gene="glrR"
                     /locus_tag="RH033_000045"
     CDS             complement(51388..52722)
                     /gene="glrR"
                     /locus_tag="RH033_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HDV2989293.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(52712..53425)
                     /gene="qseG"
                     /locus_tag="RH033_000046"
     CDS             complement(52712..53425)
                     /gene="qseG"
                     /locus_tag="RH033_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HDV2989294.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGNWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYGKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(53590..55017)
                     /gene="qseE"
                     /locus_tag="RH033_000047"
     CDS             complement(53590..55017)
                     /gene="qseE"
                     /locus_tag="RH033_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HDV2989295.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDDAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLQATACLAEPMLLMSVLDNLYSNAVYYGTESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(55574..59461)
                     /gene="purL"
                     /locus_tag="RH033_000048"
                     /gene_synonym="purI"
     CDS             complement(55574..59461)
                     /gene="purL"
                     /locus_tag="RH033_000048"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDV2989296.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHTIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYVEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEHLFAHHQPTPVTS
                     IDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTREVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APFIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEGFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            59719..61275
                     /gene="mltF"
                     /locus_tag="RH033_000049"
     CDS             59719..61275
                     /gene="mltF"
                     /locus_tag="RH033_000049"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HDV2989297.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPVGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(61272..61808)
                     /gene="tadA"
                     /locus_tag="RH033_000050"
     CDS             complement(61272..61808)
                     /gene="tadA"
                     /locus_tag="RH033_000050"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDV2989298.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(61833..62468)
                     /gene="yfhb"
                     /locus_tag="RH033_000051"
     CDS             complement(61833..62468)
                     /gene="yfhb"
                     /locus_tag="RH033_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HDV2989299.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHNEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            62677..63525
                     /locus_tag="RH033_000052"
     CDS             62677..63525
                     /locus_tag="RH033_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDV2989300.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVAMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            63581..63841
                     /gene="yfhL"
                     /locus_tag="RH033_000053"
     CDS             63581..63841
                     /gene="yfhL"
                     /locus_tag="RH033_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HDV2989301.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
     gene            complement(64035..64115)
                     /gene="shoB"
                     /locus_tag="RH033_000054"
     CDS             complement(64035..64115)
                     /gene="shoB"
                     /locus_tag="RH033_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HDV2989302.1"
                     /translation="MTDCRYLIKRIIKIIIAVLQLILLFL"
     gene            complement(64537..64917)
                     /locus_tag="RH033_000055"
     CDS             complement(64537..64917)
                     /locus_tag="RH033_000055"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDV2989303.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(64917..65648)
                     /gene="pdxJ"
                     /locus_tag="RH033_000056"
     CDS             complement(64917..65648)
                     /gene="pdxJ"
                     /locus_tag="RH033_000056"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HDV2989304.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLAEAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(65660..66388)
                     /gene="recO"
                     /locus_tag="RH033_000057"
     CDS             complement(65660..66388)
                     /gene="recO"
                     /locus_tag="RH033_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDV2989305.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(66400..67305)
                     /gene="era"
                     /locus_tag="RH033_000058"
     CDS             complement(66400..67305)
                     /gene="era"
                     /locus_tag="RH033_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDV2989306.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(67302..67982)
                     /gene="rnc"
                     /locus_tag="RH033_000059"
     CDS             complement(67302..67982)
                     /gene="rnc"
                     /locus_tag="RH033_000059"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDV2989307.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(68254..69228)
                     /gene="lepB"
                     /locus_tag="RH033_000060"
     CDS             complement(68254..69228)
                     /gene="lepB"
                     /locus_tag="RH033_000060"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDV2989308.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(69244..71043)
                     /gene="lepA"
                     /locus_tag="RH033_000061"
     CDS             complement(69244..71043)
                     /gene="lepA"
                     /locus_tag="RH033_000061"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDV2989309.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(71241..71720)
                     /gene="rseC"
                     /locus_tag="RH033_000062"
     CDS             complement(71241..71720)
                     /gene="rseC"
                     /locus_tag="RH033_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HDV2989310.1"
                     /translation="MIKEWATVVSWENGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DIAALCGAILGGVGGFLIARGCSRKFAARSEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(71717..72673)
                     /gene="rseB"
                     /locus_tag="RH033_000063"
     CDS             complement(71717..72673)
                     /gene="rseB"
                     /locus_tag="RH033_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HDV2989311.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(72673..73323)
                     /gene="rseA"
                     /locus_tag="RH033_000064"
     CDS             complement(72673..73323)
                     /gene="rseA"
                     /locus_tag="RH033_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HDV2989312.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKIRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(73356..73931)
                     /gene="rpoE"
                     /locus_tag="RH033_000065"
     CDS             complement(73356..73931)
                     /gene="rpoE"
                     /locus_tag="RH033_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HDV2989313.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(73928..74083)
                     /gene="rseD"
                     /locus_tag="RH033_000066"
     CDS             complement(73928..74083)
                     /gene="rseD"
                     /locus_tag="RH033_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HDV2989314.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            74339..75961
                     /gene="nadB"
                     /locus_tag="RH033_000067"
     CDS             74339..75961
                     /gene="nadB"
                     /locus_tag="RH033_000067"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HDV2989315.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGAESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDDRGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRCMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(75946..76683)
                     /gene="trmN"
                     /locus_tag="RH033_000068"
     CDS             complement(75946..76683)
                     /gene="trmN"
                     /locus_tag="RH033_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HDV2989316.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            76815..78149
                     /gene="srmB"
                     /locus_tag="RH033_000069"
     CDS             76815..78149
                     /gene="srmB"
                     /locus_tag="RH033_000069"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HDV2989317.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(78182..79063)
                     /locus_tag="RH033_000070"
     CDS             complement(78182..79063)
                     /locus_tag="RH033_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2989318.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVISGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQPLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVVKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEECFLPG"
     gene            79166..79753
                     /gene="eamB"
                     /locus_tag="RH033_000071"
     CDS             79166..79753
                     /gene="eamB"
                     /locus_tag="RH033_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HDV2989319.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATTHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQT
                     KPISFWASFALQFVNVKIILYGITALSTFVLPQTQALSWIIGVSVLLAVIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            complement(79808..80191)
                     /gene="grcA"
                     /locus_tag="RH033_000072"
     CDS             complement(79808..80191)
                     /gene="grcA"
                     /locus_tag="RH033_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HDV2989320.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGFAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            80496..81185
                     /gene="ung"
                     /locus_tag="RH033_000073"
     CDS             80496..81185
                     /gene="ung"
                     /locus_tag="RH033_000073"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDV2989321.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHILKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGEKPI
                     DWMPVLPAESE"
     gene            complement(81233..82270)
                     /locus_tag="RH033_000074"
     CDS             complement(81233..82270)
                     /locus_tag="RH033_000074"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HDV2989322.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEESPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            82477..82896
                     /gene="trxC"
                     /locus_tag="RH033_000075"
     CDS             82477..82896
                     /gene="trxC"
                     /locus_tag="RH033_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HDV2989323.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSHF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            82965..83663
                     /gene="tapT"
                     /locus_tag="RH033_000076"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             82965..83663
                     /gene="tapT"
                     /locus_tag="RH033_000076"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HDV2989324.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPETLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSLDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTEAATGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            83695..86355
                     /gene="pat"
                     /locus_tag="RH033_000077"
     CDS             83695..86355
                     /gene="pat"
                     /locus_tag="RH033_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HDV2989325.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKSNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQALIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RIHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            86469..87824
                     /gene="pssA"
                     /locus_tag="RH033_000078"
     CDS             86469..87824
                     /gene="pssA"
                     /locus_tag="RH033_000078"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HDV2989326.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            87868..88191
                     /locus_tag="RH033_000079"
     CDS             87868..88191
                     /locus_tag="RH033_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HDV2989327.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YAQHQGMSRDRSATFGLMFSVGLGASKELWDSRPEGSGWSWKDFTWDVAGATTGYTVW
                     QLTRH"
     gene            complement(88188..89486)
                     /gene="kgtP"
                     /locus_tag="RH033_000080"
     CDS             complement(88188..89486)
                     /gene="kgtP"
                     /locus_tag="RH033_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HDV2989328.1"
                     /translation="MAESTVMADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
BASE COUNT        21360 a        24529 c        22472 g        21430 t
ORIGIN      
        1 caaattcaac gcgtttttta gatattaaac cgtgcccatg ttggcagaaa tagtccacag
       61 caccacaagc ttttaacact tgtaacggct gatgacactc agggcaaatt gcctggacgg
      121 taaagtcttt gtcgcaagtt gcacagtggg cagtctcgcc gttacgttcg agagagtttt
      181 ggcagattgg gcaattgagt tccataaatg ctcctcacat ttcataggct tccctctcca
      241 tatggaaagg gaaaccagat aagtgattac ttacttttct tgatatgctt catcaggcgc
      301 ttacgtttac gcatctgggt tggcgtcagg gtgttacgct tgttcgcata cgggttttcc
      361 ccttctttga actgaatacg aatcggcgta cccatcacgt ccagagactt gcgaaagtag
      421 ttcatcagat agcgtttgta cgaatcaggc aggtctttca cctggttacc atgaatcacc
      481 acaatcggcg ggttataacc accggcgtgg gcatatttca gcttcacacg acgaccgcgt
      541 accagcggcg gttggtgatc ttcaacagcc atcgtcatga tgcgcgtcag catagaggtc
      601 cccacacgac gggtggagct gtcatacgct tcacgtactg attcaaacag attaccaaca
      661 ccactgccgt gcaaggctga gataaaatgc acacgagcaa aatcgataaa gcccagacgg
      721 aagtccagcg tctctttcac ctgctctttc acttcctggc tcaggccgtc ccacttgttc
      781 actacaatca caagtgagcg cccactattg agaataaagc ccagcaagga gagatcctga
      841 tcggaaatac cttcacgcgc atcaatcacc aacatcacca cgttggcatc ttcaatggcc
      901 tgcaacgttt tgattaccga gaatttctct accgcatcgg tgattttgcc gcgtttacgt
      961 acgccagcgg tgtcaatgag cacatactca cgtccatcgc gttccattgg aatgtagata
     1021 ctgtcacgcg tcgtaccagg catgtcataa acaacaacgc gctcttcacc aagaatacgg
     1081 ttagtgagtg tagacttacc tacgttcgga cgacccacaa tcgccagttt gatcggcaga
     1141 ctttgcgggt cgaagtcgtc ttcctcttct tcttcgccgt tctcttccgc ttcaaattgc
     1201 gcccagtatt cggcgtcttc gtcgacttct tcttgcggtg ccagatcttc catccacggc
     1261 agcagcacgt gctccagcag acttaatacg ccacgaccgt gagatgcggc gatcgggtag
     1321 atttccccta aaccaagcga gtagaaatca accactgcct gatcgggatc cagaccgtca
     1381 gttttgtttg ccaccaggaa ggtcggtttt tcacgggagc gcagatgttt ggcaatcgct
     1441 tcatccgccg gcatcaggcc cgcgcgcgca tccaccataa acagtacgac gtccgcttct
     1501 tcaatcgcca gcagcgactg ttccgccata cgtgtctcta caccgtcttc ggtgccatca
     1561 atcccgccgg tatcaatgca gataaactca cggccttcaa tttctgcacg accgtactta
     1621 cggtcacgag tcagacccgg gaaatccgca accagcgcat ctcgggtgcg agttagacgg
     1681 ttaaataacg tggattttcc tacgttaggg cgcccgacaa gcgcgaccac aggtaccatg
     1741 tttaaagcct catttttata aatcatcaga caacgcacgc tatattcgtg tcgttgttaa
     1801 aaacaggaaa acggcccctg tccaggagcc gttttcaaag tgaacgacag agacgattaa
     1861 cgtgtaatag agtacacggt tccgtctttt gcctggatca gcagtttgcc gtcagcggca
     1921 accggttcag tctggaaacc ggaactatca actttttgct gggcaacgaa acgaccatct
     1981 tcgacgttaa tccagtgcag ataaccttcg ctgtcaccta ccaccaggtt gccattatac
     2041 agcaccggag aagtcagcag gcgatgcagc agatcgcttt gtgtccacag cgtaacgccg
     2101 ccatcaatgg tcaacgccat cacccggtca ttttgatcga ccagatagat gcgattgccg
     2161 tcgacgatga aatcattcac cgaacccagt tcgcgtttcc acataatctg accactgcgc
     2221 agatcaagcg ccgtcaggtt accattatag gccagcgcga aaacaacgcc gttaacaacg
     2281 acgggagtcg tgtcaacatc gctcagacgg tcaatttcgg tagaaccggt cgcctgggaa
     2341 atacgctgct gccaaatcat ctggccctgt tccatcagca ctgcgctgac gcgaccatta
     2401 tcgccaccca cgacggccgc accaaaagcc gttgccggcg cagactcgcc acgcaaagag
     2461 agcgaaggca tatcgaggtt aactgtccat ttgacagcgc cgtcagcttc gttcagcgct
     2521 tgtaactgac cgttactggt gtggattaac accagaccgt cgctgaccac cggacgcgaa
     2581 agtgcttcac ccgcgacttt agtttgccat gccacagtac catcgctggt attcagcgcg
     2641 taaacctgcg ccttttcgct gccaatgtag acatgcccac cagacacggt cacgccgcca
     2701 gaaagtaatg caggatcttt agagaaccag ccatctttct cggccaggtt gacagaccag
     2761 atttctttgc cgtcatccgc attcagcgct tttactaaac cagcgcggtc cgctgcatag
     2821 acaacgttgt ccgccagtgc cggatgaaga ttggaataga agttgccaat gccgctacca
     2881 acggaagtgc tccacgcagt agtcggcgta aactggtttt caacggttgg caatggggac
     2941 atctttacca catcttcttc gctgttaaac agcgaacagc cgcttaaaag ggtaacggaa
     3001 agcagtcctg gcagcagtaa tttacgcaat tgcatcgggt ccctctcaga tggacaaatt
     3061 attaattttc atctgcatca tttcgctcag tgccggggta acatcgcttt tcacgcctgc
     3121 ttcccatgca ctacgcgcac cttgcttatc acctttgctc agcaatgctt caccacgcag
     3181 gtcggcaaca atggcagccc acccttcacc tttgatagca tcaagggttt ttagcgcggc
     3241 atcagcctgc ttgagctgta cctgaacgcg agcaagacgc agatttatca cggctttgag
     3301 attttcatcg ctcgtgtctg ccagcccctg ttgtaactgg gcggcagctt tctctagttc
     3361 atttttgtca acaaattgct gcgcaagttc caaagaagcc agcgcaccat aagtattttt
     3421 attttcagca gcaaattttt ccgccgctgg gatgctatcc ggtttgcctt cgctcactgc
     3481 ggtaaccgca ttttgatagg caagagaagc ggagcgtgca gaatcaacct gatggctgtt
     3541 ccagtagcgc cagccaatca gtgcgccaac gcccaaaatc accccaacag ccagtgcttt
     3601 gccattttca gcaaaaaagc gtttaaccgc ttcaacctgg tcgttttcgt tctcgtaaat
     3661 ttccacgctg tccttctcct tccttaaccc agtaacgtgc gcaaatgcgc ggctacgcta
     3721 tcctgcgcaa ctgcagtttg ctcaccagag cgcaaatcct tcactactgc tgtgccgtta
     3781 gccacttcag actcacccag caccacagca acgcgggcac cccatttatc agcacgggca
     3841 aactgtttct taaagttgcc gccgccgtgg ttggtcatca gtttcacgcc cggtaattca
     3901 tcacgcagac gctcagctaa tgccatagcc gcagattgtg tatcagcacc tgaagccacc
     3961 aggtatatat cgacaacagg atcggcttta aattccggat taacggcctg tactaacaat
     4021 acaagacgtt cgaggcccat cgcaaaaccg acagccggtg ttgcacgacc gcccagttgt
     4081 tccaccagac cgtcataacg accgcctgca cacacggtgc cctgggagcc gaggctgtta
     4141 gtcacccact cgaaaacggt acggttatag taatccagac cacgcaccag acgctggttt
     4201 acggtgtaag cgatccccgc gctctccagc agtttgcaca gaccggcaaa atgctcacga
     4261 gattcctcgt ccagataatc gcctaatgcc ggagcgtcgt tgagaagcgc ctgcacttcc
     4321 ggattttttg aatccagcac gcgcagcggg ttagtgtaca tgcggcgttt gcagtcttcg
     4381 tccagctttt ctttatgctg ctcaaggaat gccaccagcg catcgcggta attggcgcgt
     4441 gcttccagcg aaccgataga attcagctca agagttacgt gctcggagat acccagcgcg
     4501 cgccaccagc gggcagtgag cataatcagt tcagcgtcaa tatccggacc ttgcagaccg
     4561 aaaacttcgc agcccaactg atggaactga cgataacgcc ctttctgcgg acgctcgtga
     4621 cggaacatag gcccgatata ccacagacgc tgttcctgat tgtacagaag accatgctcg
     4681 atgccggcgc gtacacagcc cgccgtccct tcagggcgca gagtcaggct gtcgccattg
     4741 cgatcctcaa aggtgtacat ctctttttca accacgtcgg tgacttcacc aatcgcacgt
     4801 ttgaatagcg gggtctgctc tacaatcggc aagcggattt cactgtaacc gtagctgccg
     4861 agcacgtttt tcagtgtgcc ttcaatgcgc tgccagatgg ccgtttcgcc aggcaggtaa
     4921 tcgttcatgc cgcgaatggc ttgaatgttt tttgccacgt ttattctctt tctgaatata
     4981 aaaatgaacc ctcaacgctt ccctcaatgt ttcgggagcc atgcgggttc aatcatacac
     5041 gggaagcgag gcgcttccca tcacgttatt atttttcaac ctgctgaacg tcaattcgac
     5101 gcgcttcgtc cagctgactg gctttcgcac gaatgcgcgc ttccagctga tcgatcatat
     5161 cgttgttgtc cagacggtct ttgcgcacgc catcttcata gaggccgctt ttcttgttgc
     5221 cgccagtgac gccgagtgta gaaaccagcg cctcacctgg gccattcacc acgcagccga
     5281 taatcgaaac gtccatcgga gtgatgatat cttccaggcg ttgctccagc gcgttaaccg
     5341 taccgataac atcaaattcc tgacgcgaac atgtcgggca ggcgatgaag ttgatcccgc
     5401 gcgaacggat acgcagcgat ttcaaaatat cgaaaccgac tttgatctct tcgaccggat
     5461 cggccgccag cgatacgcgc agcgtgtcgc cgatgccttc agacagcagc agacctaaac
     5521 caatagcgga ttttactgcc ccgctacgcg caccaccggc ttcggtgatc cccagatgca
     5581 gcggctgatc gatttgtttt gccagcaaac gataagactc aacagcgagg aagacgtcag
     5641 acgctttcac gctgactttg aactgatcga agttcaggcg atcgagatga tcaacatgac
     5701 gcatggcaga ttccagcaac gcctgcggcg tcggttcgcc atacttttct tgcagatctt
     5761 tttccaacga tccggcatta acgccaatac ggatcggaat gtttttatcg cgcgcacagt
     5821 caaccaccat gcgaatacgc tcttcattac cgatattgcc agggttaata cgcagacaat
     5881 cgacgccgta ttccgctact ttcagcgcaa tgcgatagtc gaagtggatg tcagccacca
     5941 gcggcacgtt aacctgctgt ttgatgagtt tgaacgcttc tgccgcgtcc atcgtcggta
     6001 cggagacacg gacgatatca gcgccaacac gttccagcgc cttgatttga ttgaccgttg
     6061 cttcgacgtc tgtcgtgcgc gtattggtca tggactgtac ggcgatggga gcaccatcgc
     6121 caatcggcac attcccaacg taaatacgtg ttgattttct acgttgaatt ggagcctggt
     6181 tatgcatgaa aaatctcccg cgttacccgt ctgttactgc gccggtgatt gttcggcatt
     6241 gagggtcaga cgcgcaacct ggttagttct gataaaacga ctcagatcga caggtttccc
     6301 ttgatactgg atctgtactg cggctggcgc accaattttc agtttgtacg gtgcctggcc
     6361 ggttaagttc aaattaccgt ctttacgctg cataccgcta aacagttttt taccggtagc
     6421 atcggtgact tccagccagc aatcggcagt aaagttcatc accagcgcat tcgggtcaac
     6481 cgccggcgtg gtcacgccag cctgatcggt tggcaacggc gcagcgccat ctggtgttgt
     6541 tgttgccgct ggtgccggag tcgcagcggt atcaacattt gcctgcgaag gtggaacaac
     6601 cgcattctgt tgcggatcaa cagctggtgc tggcgcagtt acggcaggtg tttgcgtgtt
     6661 ggttgcggtg gtatccacag gtgatgtcgg cgtggtcgcc atagcctgat ctgtggttgt
     6721 cgacgtatct aacggtacac tctggctttg attattattc agttccgcag aagattgatc
     6781 ggccatagtg gtgatctctt cctgctgagc tttgtggtct tgccaccacc aggcaccgct
     6841 caggccaata accacaaaca ataccagcca ggtgaaggtc atcagccagc cgtcacgttt
     6901 tttgcggcgt ttaccgaggg aaaaactctg catcggcgca acttttgcag cccgtagtgg
     6961 agcctgcttt tccagccctg gcagcagttc ttcttctgga atatgtacca gacgcgcata
     7021 agagcggata tatccgcgca ggaatgttga agcaagatcg gcgggtgcct tatcttcttc
     7081 aatgtcgcgt accgtggaaa ccttcaggca aagtcgctcg gcaacggcct gttgactaag
     7141 tcctagttgt tcgcgagcat tacgcaggcg agcgccggta gtaagtgctt cattttggtc
     7201 gtgcgtggct tcagtattca ttcgctgcag gtacgtttaa atgagaattg agatgccggt
     7261 gaaccatcat gctcacccac accgcgaaac atccgttaag ttaaccgtta tcatacagta
     7321 taagaccgtc tgtgtgctcg tgacaaagcc cacattcgct aaggctaact tactgttgca
     7381 tcgttacata ctgccttaaa gtcagcaaaa acgcaccgtt aatattgacc agacaattgc
     7441 aacttaatac ttcattcata aaagattacg ccacggtaca taaagtaacc gtggcgtaat
     7501 ggctatcaga ccgctttaat gtcgatggct tcaccctgca tccgtttacg cagggtacgt
     7561 ttcgtacggt cgataacatc gcccgccaac tgaccacagg cagcatcaat gtcatcacca
     7621 cgcgttttac gcacaatagt ggtgaaaccg tagctcatca gcacttttga gaaacggtcg
     7681 atacggctgt tcgaactgcg tccatacggc gcgcccggga acgggttcca cgggatcagg
     7741 ttgatcttac acggcgtatc tttcagcagt tctgccagtt ggtgcgcgtg ttcagtgccg
     7801 tcgttaacgt ggtcaagcat cacgtattca atagtgactc ggccctgatt ggcgttggat
     7861 ttctccagat aacggcgcac cgccgcaagg aacgtttcga tattgtactt tttgttgatc
     7921 ggcacaattt cgtcacgaat ttcgtcgttc ggcgcgtgca gggagatagc cagcgcaacg
     7981 tcgatcatat cgcccagttt atccagcgcc ggaaccacac cggaagtgga aagcgtgacg
     8041 cgacgttttg acaggccaaa accgaaatca tcaagcatga tttccatcgc cggaacgacg
     8101 ttgttcaggt tgagcagcgg ctcgcccata cccatcatca ctacgttagt gattgggcgc
     8161 tgaccggtga cttttgctgc gccgacaatt ttcgccgcac gccacacctg gccgataatt
     8221 tccgataccc gcaggttgcg gttaaagccc tgctgggcgg tggaacagaa tttacactcc
     8281 agcgcacacc ctacctgcga agagacgcag agcgtggcac ggtcgtcttc cgggatatac
     8341 accgtttcaa cgcgctgatc gccaacggcg atcgcccatt taatggtgcc gtcagatgaa
     8401 cgctgttctt caaccacttc cggtgcacgg atttccgcca cctctttcag tttgccgcgc
     8461 aacactttat tgatgtcggt catctcatca aagttgtcgc agcaatagtg atacatccac
     8521 ttcatcacct gatcggcgcg gaagggtttt tcacctaaat ctttaaaaaa ctcccgcatc
     8581 tgctgacggt tgagatccag caggttgatt tttccatctt tcgtggtgac gttttcaggt
     8641 gtgactaatt gttcagacat atgctattcc ggcctcgtta ttacacgtta tggcccctgg
     8701 agggttgaaa aaagaaacgc cccggtgagc ggctgctcgt ccgggggcgc tgcattgtac
     8761 aaattctggc gcacggatgc cacgtttgca cgcggcattt acgaaattat taacgggtgc
     8821 gcgggcacac ttcgccttcg ccgaagaaat aagcgatttc gcgagcggca gattcgacgg
     8881 aatcagaacc gtgggtacca ttttcggtca ggctgtcagc gtaatcagca cgcagagtac
     8941 cggccagtgc gtttgccgga ttggtcgcgc ccagcagatc gcggtgacgc tgaacagcat
     9001 tttcaccttc cagcacggaa acaacgatcg ggccagaagt catgaattca accagaccat
     9061 caaagaacgg ttttccatcg tgttcagcat aaaagccacg agcctgttca acggtcaggt
     9121 gcagcatttt ggtgccgaca attttgaacc ctgcagcttc aaagcgcgca aagatactac
     9181 caatcacgtt ttttgctacc gcgttcggtt tgatgatgga aaaagtacgt tcaatagcca
     9241 tttttacctc tgtaaattgt tctgttgttg tctgtaccag cgtacgagat ggcgcggatt
     9301 ataatgagca acagagccgt tgactattga tgaaggtaac atttttttaa aataaaccca
     9361 gtttaagcaa caatcaggga caaaacagac tattgcatga caaatttcac tgtcgctatt
     9421 tgccccacgt catccatcac cagcaattga taatcgcctt tatccgtcaa atgcagggtc
     9481 acgttgcgcc cacgttcagt taacggttcg ccgttcagaa accaccagcg ttcacctgcc
     9541 cctccacttg attgcaacgg caaagtcgct tccgctgcgc ccggtaaacg tttaataatc
     9601 gcgccatcgc gcacacctgt tagctgcagc gggagttgtg catcgtgacc gtatggcggg
     9661 caaattgtcg aggccggtgg caagcgcaca gcgcgacgtt ctgatgcggg cagccagggt
     9721 tccagcggta gcggccagac atttatcatt tcttgtcgcg cctgagggca atcagcggca
     9781 acacgtttgc cgttttcatc cagccagatg gggaaacgaa tgccattaat gccttcctgc
     9841 tccggcagta atagagttgg cggctggctc ccgtccagca gccaggttgc caggcggcgg
     9901 cgacagttag cgtcaccttc cggcaaagac tgtccgcccg gccagcagat aacgccacga
     9961 cttacagagt aaggacgtgg gtcttccggc aaattcacac tgcgcgacag taagatatta
    10021 ttaacctgat tcaacagcgg tacggcactg gcaaagccaa actgaccaac aacgggcgtg
    10081 ccgtccggtc tgccagtcca gatcccaatg acatagcgag cgttaacccc aatcgcccag
    10141 gcatcacgat agccatagct ggtgcccgtt ttccatgcca gtggggcgac gcgcggcaag
    10201 tcaccatccg gcaagggttg cgcttcatca gccataatcc ggcgaatgat ccacgcagcc
    10261 cccgacgaca ttaaaggccg ttcaaacagc ggatcgtcag gctgtaagcg caatttgcct
    10321 gccttgccgt ggcgagcaaa cgcggtatac gctgccgcca tatcttccag ttttgcgcca
    10381 gcaccgccga gaatgagcga aagattcggc gcagcaccgt tgggcagata caacggcaat
    10441 ccaacattgc gtaactttgc cgcaaaccgt ttcggtccat aggcttccag cacctgcaca
    10501 gcaggtaagt tcagcgatcg caccagcgcc tcactcatgc tgatcgggcc atgaaaaccg
    10561 ctgtcaaagt tgccaggtcg gtaatcaccg gtgcgtcggg ggacgtcttg caacagcgat
    10621 gccgggtgga tcaagccttc atccagcgcc agaccgtaaa caaacggttt gagcaccgat
    10681 cctggcgatc ggatcgcatt gaccatatca acatgaccaa agcgtgaatc atcgttgaga
    10741 tcaaccgaac ccacccagcc gcgaacacgc atatcggtat gatcaaccac gatcatcgcc
    10801 agtgagctgc gcggtggtaa tcgtcctttc cagttttgcg ccagttcttc cagacgtcgt
    10861 tgaagaccgg catccagcgt agtaacgatt ttgtcgcttt tgcttttacc gagcatcatg
    10921 cgcgaaaaca gcggtgccag ttgcggcatt tgtcggggtg ccagccagat gggttcttcc
    10981 cttgactctt ttacccgctc acgggaccac acaccttgca cggccatccg ttcgagcact
    11041 ttattacgcg cggcttcggc acgctccggc caacgatccg ggcgaagacg gctgggcgct
    11101 tgcggcaaaa ccgccagcat tgccgcctcg gaatagctta aattcgcagg cgatttcccg
    11161 aggtaagccc aacttgccgc accaatcccc tgcaacgtac cgccaaacgg ggcacggtta
    11221 agatacaagg tcagaatttc acgcttagac agatgccatt ccagttgcaa cgcgcgccag
    11281 agctggcgaa ttttgccgcc aaacgttttg ggatgaggat caagcaggcg cgccacctgc
    11341 atggtgagcg tgctgccgcc ggaaatgacc cgacccgacg tgagatcttg ccaggctgca
    11401 cgcgccaccg agaacggatt cacccccgga tgcttccaga accagcgatc ttcataattg
    11461 atcagcgctt caaggtaacg tggagaaaca tcttcgattg ttaccggata acgccagatg
    11521 ccgtcagcat cggcgaaacg ccagagtggc gtaccatcct gcgccaccac cacgcgtgca
    11581 ggattcactt cttgcaatgg cagaggccag agtttatctg ctgcccatgc tgccagaatg
    11641 atgataaaag gcgcggctgc cagcattatc cagcagccgc gtttggttaa caggcgaggc
    11701 atttacggtc tgactatcaa cagatcgtca gccgcaccag tcgcccgcca ttgaggaaca
    11761 tacattgatt ccaccatagg ctgcggcacc tgatacgttc ccggcgtcac cgcccgcgcc
    11821 agatacacca gcgttaccgg ttgatattca tcaacggcaa ccgccgccac aaagcgatcg
    11881 tcacggaact ctatgtgctt aatgctcgcc tgctgcatct ggttcagcaa gttctgcact
    11941 tcaccaccac tttgctccag gctggcgctg ccgttcgcca gattctggtt ttccagttcc
    12001 agccccgcag acaacagatc cacgactaac gcatccggta cactgttact ggctttaacc
    12061 tgcaaccaca ccagcaccag atcgccgcta cgtaacgagt ccagcgattt gctcttaccg
    12121 tcagtaccca gaatatgacg ctcaatttgc agcacattgc tcgccggtaa aggtgcggat
    12181 tgcggataac cgctggcatc cacacgcagc cataacggct gatcgccact gttggtcacc
    12241 tgcaaggtgg caagttgatc gctattcaga ttgctggttt gcgctttctc gcctgtcagc
    12301 ggctcagcag agaaagtggt ttgcgcctgc catttgcccg gtaaatcctg aagagtacgg
    12361 gcagccagga acaacgcgtt actttcctgc gtcgatagcc agcgttcacc gaacgcctgc
    12421 tgcgaaagtg tgttcaacag ggagtactgc tcatcgggta gcagtttgtt ctcttccagc
    12481 aatgagagca tcaacgcgct gtcgcgcagt ggactaccgt aatcacccag ccatatccgc
    12541 tcatcactat tacgcggcgt tttcagcgcc agcacaatcg cttcttcacc acgcattgca
    12601 tcacccatgg ttttcagcgc aacgccaagt tgcagcagcg gtaaaccaga agcggcatct
    12661 gcacgatgct cccagatttc acgcagcgca cccagtggtg ctttttgctg acgggccaac
    12721 accagcgcag cgtaagactg tacggcgaat ttactggctt tgagattatc agcgtacggg
    12781 atcgatatca tgcccggatc ttgtaaatag cgcagcagac gctcattacc ccggttaatg
    12841 gcgtctgtcg gcacgctgta accctgttcg cctgcgcgga ccaggaaatc catcacgtaa
    12901 gccgtcagcc agtactcttc gtcaccgttt ttatcccaca gcgcaaagcc gccgttatca
    12961 cgttgcattt gcagcaaacg ggaaatgccg atatcgaccg atgcacggcg tttctcatca
    13021 ctgtcgcctt tgatgcccaa cgcctgcagt tgggctgcat tggtataaag tgacgggaac
    13081 aggccgctgg cggtttgctc gagacagccg tacggatacg cttttaactc tttgatataa
    13141 cgtgcgatgt tcagcggtgg tttgccgctc aacaacaatt gcccttccag cgtaacaggc
    13201 gagaagtttt gcaatccatc agccgggagc gcccaggttt caccaggctg taacgccgta
    13261 ccgtaattga ccgtttgtgc cgggaatgcc ggacggacgc cgattgtcca ctgcttatgc
    13321 tgatcggcaa cggtttcccc cggtaacgct aacccgctaa tggtggcctg aatgtctcca
    13381 tcgccataac ccggcaatgc tcgcactggt ataaacagcg tagtacgcac acctggcgct
    13441 aattcaacgg ctgcgggtga atcgctgacc agttcaagca aaccactggc ggtcagggca
    13501 acgttcagtt tttgcggttt atcggtaaga ttagtgatat ccagcgtcag acgcgaggta
    13561 tcgccactcg ccataaagcg tggcatgttc agttcagcaa tcaccggtgc ggcaactatc
    13621 actttgcttt cgttgctacc gaagtcatct gccgtccagg cttgcgccat gacgcgcaat
    13681 tcaccgttaa aatcaccaat cggcagtgta accgagcctt caccctgttc gttgagcgtt
    13741 accggcagcg cctgctgcgc gacaatattg acgtgattga ccggcggttt accaccacgt
    13801 ttcagctcat caccatcgcc accaaaacgc agagctgcca gacgcccctg accttcaatg
    13861 acctgaccgt aaatatcgta aatgtctgca ccatagcgtt tctgaccaaa gaacgcctgc
    13921 cacggatcgg gggtaacgta atcagtaata ttcagcacac cgctatcaac ggcagacacc
    13981 agcacattca cctgttttgg catctcacca tttttattgc tggctttaat tttcacggtt
    14041 aatggctgat tggggcgcat tttggtgggg gtttccagcg ccagatcgag acgacggttt
    14101 tcatcgccaa gcggcagatg caacacccca actgcgcgtt ttggcgtcgc ggagcgagat
    14161 ttatcgccag gacgtaccac cagcgtactt aaatacaaat catgacgatt ccaggtttta
    14221 tcgaccggaa tcgtcagatc cagcccttga gccggaacat caatctcttg ccaccacagc
    14281 ggcccttcac tggactcgac catcgcataa cctttacccg ccgttggcgc ggcaatatgc
    14341 aacttaatgg tatcgccagg gcgataactg gctttatcca gtttcagcgt gacacggtcg
    14401 ggtcgcacgg caccgctccc gtcgctgttg tcctgccagc tatagccagc ccagaaacgc
    14461 acactactga ccgcttcatt cggcgcttta acttccagac gataagcgcc ccactctacc
    14521 gggaaactga ccttaccggt ttcgtccgct ttcagatcca gagtttgttc attttcgatc
    14581 agatcttttt gctcaaactg tgactgccag ccttcatatt ccgaccagtt ccagtagtaa
    14641 tcgcggcgtt cgcgaatcag gcgcacctgc aagcccgata cggctttttt cacgccttgc
    14701 gcatcgctat aaacgatgtc aaaagcggcg ttactgcctt catcaacaat gggctgtttc
    14761 accgtgctgt cggtgcgata atcgtaaacc gatttcgagg cgaactgcgg acggatcccc
    14821 ggcaatgcat cagcaggcca gatagcctgc tcagcgcggc gcgtcaccgg gcgaccgccc
    14881 gattccaaca aactaccctg gaaaataacc tgtaatgggg aatgcgtttc cttccactgg
    14941 ctttctgtag aaacttcgcc acgcccttta tcatccagcg tcaactgaac ttcatccagc
    15001 gtgcgggaaa gattttcggc agctatatcg ccgaattcga aaccaggtaa ggctgacaca
    15061 gcttcacgca gtgggcgcag gaaaagttgc ccttgcaaag tattaccatt agcaggcgca
    15121 ccatacaggt agtaccccac cacggagaat ctcacttcat ctttcggcgt tagcggggtt
    15181 ttctcaccgg tcagattcag cgccatgcgc tctggcataa aatcttcgac gtggaaatcc
    15241 cacatccgat actggttatc gcccgtgtta gcgcgaatat gccacatacc ggttgccgca
    15301 ttgctatcga gtggccaggt aaagtggtag aggccattct ccggctgact aacgacgctc
    15361 ctgagtacct gcccgtcggg tttaatcacg tctaacttga tgggttgatc gggcaatgct
    15421 ttaccatctg catcacgcaa taaaccattg aggatgactg tttcaccagg gcgataaaga
    15481 tcgcgcgggc caaacatgaa aaactgtttg ctatagcctg gcgcgccagc aatgttaaat
    15541 tctgctaagt ccagcgccgg aagttttaaa tcgagtagcg ttgtctgacc gtctttacgc
    15601 gccaacagta atgccgcgtt tttatcattt tccagctgca catgcccctg ggcatcactg
    15661 gttgcctgag tcagagtctg ccctttctca tttaataaag agacttcaat tccttgctgg
    15721 gctgcgccgt tttccagact ttgggtaaag atatccagac gattgtgata acggtgagct
    15781 gaaacgccaa tatcacttaa cgtaaacagc gtcgcgggat tactgtaatc gtaacgtcca
    15841 gcctgattca tcacagccag atacacgccc gcctgctgaa gcggtttgat atcgcccagc
    15901 ggcagcaata atttttcacg ggtgttacgc gcaggattga gatcaaaccg tccggtgtag
    15961 accagatccg ccatctgcag cagtttatct gactgccagt tcgccagcga attgcggtat
    16021 tcccactggc taatgaaagc gggcagtgat tctggtttca cacggaagaa gttaacatcg
    16081 acattgttaa cgttaagcgc cattaccggc agcccttcaa cgaccttacc aggaagcaac
    16141 gaaccacgac tggcaaaacc gacgctgggt tggatatcgc gggtagttat agttttttcg
    16201 tcatctttac taaaggttgc gttattgagc gctttgacct ccttgccaat agtgacgatc
    16261 aaatcacgtt tcggttcgag gtggcgtaaa cgcaactctt taagattatc tgacagctcc
    16321 caggcaccat ccactttgcc gcttttctta tcgacgacat ggataacgcg tgagaattcc
    16381 tgatccgggt cgagagggat ggagaatgtc agcaccagcg tggctgcgcc atcaagttga
    16441 acttcagaga gatccagcaa tgtcagcgcc ttaccggcac tctgttgggc cagtttttgt
    16501 cgctctggcg cggaaagttt tgctgaactc acgttttctg aagaggccgc tttagtaact
    16561 tcagaaggcg catctttttt caccgctgtt ggcgcgttat cgttgttgtc gcaccctgcc
    16621 agcgccagca ttagcatgca ggcggctacg cgtaactttt tcatttttca tccctgccac
    16681 aatggcccgt tagcaacgtc gaataattat tatgcgtgag aatttacgtt atgtaattca
    16741 gtatcacgcc catacaatcc gaattttagt gagaattgtg gctcagacag ccaaatcagc
    16801 ctcttttgct tgtcgccttt cactaaacat ccgacaattt agcctaaccc cggcaaaaat
    16861 ggagatgcct atgtccacga catggtttgt aggagccgac tggctcgccg aacatattga
    16921 tgacccggaa attcagatta tcgatgcccg catggcgtcg cctggacagg aagatcgtaa
    16981 cgttgctcag gagtatctga atggacatat tcccggcgca gtgttttttg atatagaagc
    17041 gctttctgat cacacttccc cgcttccgca catgctgccg cgcccggaaa cgttcgccgt
    17101 ggcgatgcgt gaattaggcg ttaaccagga taagcacctg attgtctatg acgaaggtaa
    17161 tcttttctca gccccacgag catggtggat gctgcgcacc tttggtgtag agaaagtgtc
    17221 gattctgggt ggtggacttg caggctggca gcgtgatgat ctgctattag aagaaggcgc
    17281 agtagagctg ccggaaggag agtttaacgc cgcgtttaat cctgaagccg tggtgaaagt
    17341 aaccgatgtg ttattggcaa gccatgaaaa tacggcgcaa attatcgatg cccgcccggc
    17401 tgcacgtttt aacgcagaag ttgatgaacc tcgcccaggt ttacgtcgcg gacatattcc
    17461 cggtgcacta aatgttccgt ggacggaact ggtgcgcgaa ggtgaactaa aaacgaccga
    17521 tgaactggat gcgatatttt ttggtcgcgg cgtaagttat gacaaaccta ttatcgtcag
    17581 ctgcggctct ggtgtaacgg cagccgtggt tttgttggca ctcgcgacac ttgatgtgcc
    17641 aaacgtgaaa ctgtacgacg gcgcatggag tgaatggggc gcacgagcag atttaccagt
    17701 tgagccagag aaataagctt ttaacaggca ataaaaaacc gccgaatctg gcggtttttt
    17761 attgctggtc tggttcgcgg cctttccagc agattggctt gtgttgcatg agcaacgcag
    17821 gtgcttcaca gcaaagcaat actcgccagt aactctcttt ttgtcaagcg aaagagagta
    17881 atcattgttt atttagcgta ttatcgacac cggccctttc cgccgtgttc ggtaataaaa
    17941 taacctggct tattagtccg aattcagaaa aatataaaca aatcctgctc aaaattaaaa
    18001 atactaaccg gtaaaagata ttacttaaac atgtaaattc actttccttt aaaaaacaaa
    18061 aaaccgccaa aatcaggcgg ttatttgttg ctggtttggt tcgcggcctt tccagcagat
    18121 tgtattaccg tagtaatgca agcgcgtctc agcggacaca atactcgcca gtaactctct
    18181 ttttgtcaag taaaagagag ttattattgt tctgttagtg tattatccac tgcggacctt
    18241 tccgccgtct cgcaagcggg cgctggctta aggaaaggat gttccgcgac cgttagagca
    18301 agcgcgtctc agcgcttgct atcgcggcaa tatcgccagt ggtgctgtcg tgatgcggtc
    18361 ttcgcatgga ccgcacaatg aagatacggt gcttttgtat cgtacttatt gtttctggtg
    18421 cgctgttaag cgaggtaaat aataaccgga gtctctccgg caacaattta ctggcggtta
    18481 acaaccttca gagcagcaag taagcccgaa tgccgccctt tggggcggca tattttagat
    18541 tatccggttc tgtttaaagt cacgcaaaaa accaccccag cgacgttcat agaatggcgc
    18601 aatatgttcg gtaataaagt ggctaattcc tttttcccct tttttcacct gacaaatatc
    18661 gatgggttca tcgccaggta atgtatcggt cgctacactt cccgccgcct gaataatttc
    18721 ttcgatatca ccatcggctt caatgccaat aagtaaatta ggctgtgcct cttcgttctc
    18781 tttaattgaa caaataaaag cacgcttcac cggcttaatg gttttaaata aggtggtgag
    18841 tgaatcaatc atttgtgctg gtggctctgc gacttccgat aatatcagcg attcaccgcc
    18901 ttccagtact tcctggctgc tcagcggatt tccttcttca ccaatcagca aactgatttc
    18961 acgcggcata aattctttac cggttggcag tttggcatta aggaagagcg tttcgccaag
    19021 ggtcatctca aacaatgtgc gaacgggcat tacgacaaat gcctgttcgt cttcaaccgc
    19081 ctgttgaagt gcttctaacg aggtaaaaaa aggaatgacg ctggtgcagt cttctttttc
    19141 ccagtgctgt aaatcaagcg cgctatcttc aaccacagcc tcgccctgcg ccgccgtacc
    19201 cggcacccag acggtggatt ccagtagagt acggaaaaag gccgggcggt gcgccggttc
    19261 ggttgctgct ttttccagca ggtcttcaag ttcgtttttt gtttcggaca taagaaccac
    19321 aattcattca acgttcgggg cacaagtgcc tgatgcgcta cgcttatcag gcctacaagg
    19381 gaatctcaat ctattggatt ttcgcaattt tgtaggccaa ataagacgtt tacgccgtta
    19441 acagattagc aatcgtgcgc acacctagcc cggtcgcgcc cgcagaccac tgttcaaccg
    19501 gcgctttacg gtaagtcgcc gagcagtcga tatgcagcca gccttgctga tagttctcaa
    19561 caaagtgcga caggaagccc gccgccgtgc ttgcacctgc cggatacgcc gcgcttccgg
    19621 tattgttcag ttcggcaaag ttagacggca gctggctgcg gtggaactcc gccagcggca
    19681 gacgccagaa tggttcattc tcctgcgcgg cactcgccag caagcgagcg gcaagcgcat
    19741 cgtcaaaact gaacagcgcg tgataatcat tacccagcgc ggttttcgcc gccccggtga
    19801 gggtcgccgc atcaatgatc agttccggtt tctgcgcact ggcatcaatc aggccatcgg
    19861 ccagcaccag acgcccttcc gcatcggtgt tcatcacttc aactttttta ccgttgcgat
    19921 aggtaatgat atcgcccagc ttgaacgcat tgccgctgat caggttatcc gcacagcaga
    19981 ggaacagctt cacgcgcttg ttcagtccgc gcgtaatggc aaacgccagc gctccggtaa
    20041 ccgttgccgc gccgcccatg tccgacttca tcgagtccat aaatgcggtc tgtttgatac
    20101 tgtagccgcc ggaatcaaag gtgatgcctt taccaaccag gcacgcgtac actggcgctt
    20161 ctttatcgcc tgttgggttg taatccagcg ccagcaatac cggggaacgt tctgaaccgc
    20221 gcccgaccgt gtgcagcccc atataacctt gatcacgcag atcttcgcct ttggtgatcc
    20281 ggtaagtcac acgatcgccc gcgacattgc tgatcagatc aacagcacgc tgtgccagtt
    20341 gcgatggccc cagttcttcc gccggtgcgt tgatggtgtc acgcacccag tcgatgatca
    20401 tcaggcggtt atccagttcc tgccgttggg catcgtccag atccggccac tccactttac
    20461 gtgtgccttt cggggctttg taaccttgcc agaatgccca gcagcgatcc gcatcccagt
    20521 cttcaccgct taactgaaca tgcttgatgc ccagaccgtc aatcttgcgc gccgcacgct
    20581 ggatcagccc cagatcgtct gtcccgttca aatgcagggt aatgccatca ttattaatgc
    20641 tgtaagtcgc tttttctccc cagcgcgcgt cggcaggttg ggtagaaagg gtaattttca
    20701 tcgcttctgt cattttagtt atccttattc gtttaacagc aaaacgggct acccccaggc
    20761 agcccgttca ttcactcggc ttcgtccagc cagactaaca aaatcgcttc gaggattttc
    20821 tcgttggatg cctgcgggtc gtcgtcgaaa tcttccagat cgcaaatcca ctgatgcata
    20881 tcggtgaatc gaaccgtttt cggatcaaga tcgggatacg catcgtacag tgcttcgcca
    20941 atttcgcggc tatcggtcca cttaagtccc atactaacct ctgttaatgc tcacgcgcat
    21001 ggttgatagt gtaacgcggg atttcgacca ctaaatcttc gtcggtaacg cgcgcctggc
    21061 agcttaaacg gctttccggc tccagtcccc aggctttgtc cagcatgtcg tcttcctgct
    21121 ctgagctttc cggcagcgag tcaaaacctt cgcgaacgat gcagtggcag gtggtgcaag
    21181 cacaggattt ttcacaggcg tgttcaatct cgataccgtt acgcagcgca acgtcgagaa
    21241 tggtttcacc actattcgct tccagaacag cgccatcagg gcagagatcc tgatgaggca
    21301 aaataacaat ctttggcata ttaaacctcg tccacggaat ggcctttcag cgcacgacga
    21361 accgactggt ccatgcggcg agcggcgaaa tcctgggttt gtttgtctac gtttttaatc
    21421 gcttgttcga tggcgtcaac atcatcgccc tgcgccactt cactcaggtg agcggcagca
    21481 tcgtcaatga cctgacgctc tgcggtgctt aacagcgcgg catcagcagc cagcgcgccg
    21541 tgcaggcttt ccagcacacg cgccgcttct actttttgtt ctgccagcat tcgggctttt
    21601 acgtcctgct cggcatagct cattgagtct ttgatcatcg aagcgatttc gctatcggtc
    21661 agaccgtaag acggtttgac ctgaatagac gcctcaacgc cggtggattt ctccatcgcc
    21721 gtcacgctca aaagaccgtc ggcatcgacc tggaacgtca cgcgaatatg cgcgccgcca
    21781 gccggtagcg ccggaatacc acgcagcgca aaacgcgcca gcgagcggca gtcctgcacc
    21841 agttcgcgct caccctgcat tacatggata gacatcgccg tctgaccatc tttaaaggtg
    21901 gtgaaatcct gagcgcgcgc caccggaata gtggtattac gcggaatcac tttctccacc
    21961 aggccgccca tcgtttcgag gcccagcgac agcgggatca catccagcaa cagcatttcg
    22021 ctgtccggct tgttacccac cagaatatcc gcctgaatcg ccgcgccaat agcgacgact
    22081 ttatccgggt cgatagaagt cagcggtgga cgaccgaaaa attcgcctac tcgttcacgc
    22141 accagcggca cgcgagtaga accgccaacc atcaccactt ccagcacttc atcagcttct
    22201 acgccagcgt ctttcagcgc gcgacgacaa gccagtaagg ttcgttttac cagtggcgcg
    22261 atcagttcat tgaattgttc acggctgatt tcgccctgcc agcccgcaac gttaacggtc
    22321 acggagtccg catcgctcag cgcgattttg gctgcaatgg cggtatccag cagttcacgc
    22381 tgaacgcggt tatcgctacg atcaggaatg cccgccttct cgcgaatgta atccgccagc
    22441 agatggtcga aatcatcgcc gccgagcgcg gaatcaccgc cggttgccag cacttcaaac
    22501 acgccgcgac ttaagcgcag aatggaaata tcaaacgtcc cgccaccgag gtcataaacg
    22561 gcgatcacgc cttcctgacc ggaatccagc ccgtaggcga tagccgcagc ggtcggttcg
    22621 ttaagtaagc gcagaacatg taaccccgcc agacgcgccg cgtctttggt gccctgacgt
    22681 tgggcatcgt caaagtacgc cggaacggtg ataactacac catccagctc gcctgccagg
    22741 gcttcagttg cccgcgccgc cagtgctttg aggatgtccg cagaaacgcg caccgggttc
    22801 agcagccccg ccgccgtttc aatcatcggc aggccgtttt cgctagcctg gaattgataa
    22861 ggcagatgcg gatagcgttg ctggatatca gccagcgagc gccccatcag gcgtttaaca
    22921 gaactaatgg tgttggcggt gtcaagcgct gcgttagtac gcgcgtcata acccaccgaa
    22981 tgcccttgct gttgatagtg aacaacagac ggcagcaggt gacggccttc atggtcggct
    23041 aacgtttcgg cctgaccgct gcgcactgtc gccaccagcg agttggttgt gcccaggtca
    23101 ataccggccg ccagacgacg ctgatgcggc gcagcactca aaccaggttc actaatttgt
    23161 aataaggcca tgtttagctt ccagaaatta aaaatcgagc agtttttctt cgagttgttc
    23221 ggcactgctt cgcagtttat cgagaaaacg cagcttacgc acggtatccg ccgccgcgtc
    23281 ccacgtctcg ttgtctaact gttcaaccat caactgatgg cgggtatcaa acatcttttt
    23341 cacacgtttg ataaagcttt ccagccgcgc ttcatctttc gcctgttcga tctcgtccag
    23401 ttcttcgcgc agttccagct gttccatcag gaacgcggtg tcgcgcacag tatgctgctc
    23461 gctggcgaga tcaaaaccgt gcaaagaaag caaatattcc gcgcgcatta acggatgacg
    23521 cagcgtttgc caggcctggt taatggttgc agattgctgt acggcggcga gttgttccgc
    23581 ctggcttccg ctggcgaatt tatcaggatg atactgacgt tgtagatcct gaaaacgcag
    23641 gctcagcgcc tgggtatcga gttgataacg ggcaggcaag ccaaagaggg tgaagtaatc
    23701 cataacattc tcagggctgc gatccgccgc gataacggcg aatcaggtaa aacaaacccc
    23761 acgcgcaggc gaccacggtg gggttatcgg tatgcgcatc aaacgtggaa gctttcgccg
    23821 caaccacact catctttaac gttcgggttg gtgaatttaa acccttcgtt caggccttct
    23881 tttacgaagt ccagctgcgt accgtccaga aattgcaggc ttttgccatc gaccacgact
    23941 ttcacgcctt tgtcttcaaa cacgatgtct tccggcgtcg gttcgtcaac aaattccagt
    24001 acataagcca tacctgaaca tccggaggtt ctcacgccca gacgcaggcc aaaccctttg
    24061 ccgcggttag ccagaaaggt atttactcgc gctgctgcac tgtcgctcag tgtaatcgac
    24121 ataaccaaac ctcaactctt attttgcttc acgtttgctt ttatagtccg caatggcggc
    24181 tttgatcgcg tcttctgcca gaatagaaca gtgaattttc accggcggca gttcaagttc
    24241 ttcagcaata tcggtgtttt tgatcgcctg cgcttcgtcg agagacttcc ctttcaccca
    24301 ttcggtgacc agggagctgg aagcgatagc agaaccgcag ccgtaagttt taaaacgcgc
    24361 gtcttcaatg ataccttcat cgttgacttt aatctgcaac ttcatcacgt cgccacaggc
    24421 cggtgcaccc accatgccgc tgccgacgtt ctcgtcgttg ttgtcaaagg aacccacgtt
    24481 acgtggattc tcgtaatggt cgataacttt ttcgctgtaa gccattataa attctcctga
    24541 ttccgatacc gattaatgat gtgcccattc gatgctgttc agatccacgc cctgcttgta
    24601 catttcccac agcggagaaa ggtcacgcag acgaccgatg gatttacgaa ctaactcgat
    24661 ggtgtagtcg atctcttctt cagtagtaaa acgacctaaa gagaaacgga tagagctatg
    24721 tgccagctcg tcgttcagcc ccagcgcgcg cagcacgtag gacggttcga ggcttgctga
    24781 cgtacaggcg gaacctgaag aaaccgcgag gtctttcagc gccataatca gcgactcacc
    24841 ttcaacgtag ttgaagctga cgttgagaat gttcggcgca ccgtgttcca ggtcaccgtt
    24901 caggtaaact tcttcgatat ctttgatgcc gttccacaga cggttacgca ggccgcgcag
    24961 acgttccatc tcggtcgcca tctcttcttt tgcgatgcga taggcttcgc ccataccaac
    25021 gatctggtga acaggcagag tgccggaacg cataccgcgc tcgtgaccgc cgccgtgcat
    25081 ttgcgcttcg atgcgtacgc gcggtttacg acgcacatac agcgcaccga tacctttcgg
    25141 gccatagatt ttgtgaccgg agaatgacat caggtcaact ttcaactggc tcaggtcgat
    25201 aggcagtttg cccacgctct gggttgcatc aacgtgatag ataatgccgc gagcacggca
    25261 catttcgccg atagccgcga tatcctgcac cacgccgatt tcgttattta cgtgcatgat
    25321 ggaaacgagg atggtgtcgt cacgcatcgc tgcttccagt tctttcaggt cgataatgcc
    25381 attacgctgc ggtgccaggt aggtgacttc gaaaccttcg cgctccagtt gacggcaggt
    25441 atccagtacc gctttgtgtt cggttttact ggtgatgatg tgcttgcctt ttttctgata
    25501 aaagttggct gcacctttga tcgccaggtt gtcagattcg gttgcaccag aggtaaagac
    25561 gatttcacgc gggtcagcgc cgaccagatc ggcaatctga ttacgggcga tatctaccgc
    25621 ttcttcagcc tgccagccga aacggtgaga acgggaggcc gggttaccaa aggttccgtc
    25681 catcgtcata aactgcatca ttttctcggc aacacgcggg tccaccggcg tggttgcgga
    25741 gtagtcgaga taaatcggta atttcattgc tctataaact ccgtacatca ctcaatgcaa
    25801 ggaaccaggc taccggctgg atgtacgacc gtgtttacgg agcgcgatac gccccggcct
    25861 gattctgaat tctttttatt aagcgcgtaa cttaacgtcg atcgcgtctt gtgtgcgggt
    25921 gcgtggcgcg tcgtgagtat gctgacgacc agacacatcc agcacttcct ggttattaac
    25981 cagttcgcct aaagtaatgt tgttgagaaa accagtgaga cggtcgctca aatcacgcca
    26041 cagcgcgtgg gtcaggcatt tatcgccgcc ctggcagccg cctttaccct gacaacgggt
    26101 ggcatctaca gattcgtcaa cggcgctaat cacttcgcca acggcgatgc tgctggcatc
    26161 tttgcctaac agataaccac cgcctggtcc acgtacgctg gaaaccagac catttttacg
    26221 cagacgggaa aacagttgtt ccagataaga aagggaaatt ccctgacgtt cggaaatatc
    26281 agccaacggt accgggcccg cttcagagtt gagcgcaacg tcaagcattg cggtcacggc
    26341 atagcgccct ttagatgtca gtctcatgtc ttacttcacc tcaaactcgc ccctgcccgg
    26401 ggttttttat tgtaaaagtg ggggtattgc atagcagggt caagtctgac attcccgagt
    26461 aaattggtca actatttact tgactgattt agtcgggtat ttaaccttca gtgccatttt
    26521 ttatcgtggc gtttgtacct gttgtcggat gcggtgtgaa caccttatcc gacctgggat
    26581 tccgtgcact tacaggcctg ataagacgcg ttaagcatcg catcaggcaa ccactgttat
    26641 tcggccttgt tacctttatt ctgctgctca atagaagcca gaatcccgcg caggatattc
    26701 aattcctggc tttccgggcg tgcacgggta aacagacgac gcaatttatt catcacctgc
    26761 cccggatggt tttcacgaat aaaaccggtt gccagcagcg tttgttccag atgaccataa
    26821 aaacgctcca gatcatcgac cagcggatac ggcgtctctt catgctcggc ctgctcacca
    26881 ttttcctgag ttgccagcca ggccatgcgc acttcatagg caataacctg aaccgccatc
    26941 gccagattca gcgaactgta ttccgggtta gcggcaatcg cgacatgata atggcatttc
    27001 tgcaactctt cattggtcaa gccgacgcgc tcgcgaccaa aaaccagcgc caccggggta
    27061 tttgccgctt cagcgacgct tttcagaccg cattcgcgcg ggtcgagcat cggccacggc
    27121 agcgtgcggg agcgtgcgct ggtgcccacc accaggctac aaccggctaa cgcttcgtcg
    27181 agcgtatcga cgatgtgagc attaccaatc acatcgctgg cccctgctgc cagggcaatc
    27241 gcctgggagt caggtttcac cagtggatta accagccaca gattggttaa tcccattgtt
    27301 ttcatggcac gggcaacaga ccccatattg ccggtgtgtg acgtctccac cagcacaatt
    27361 cgaatatttt gcagcattgt ctttcttcat ctaaagatta ttcacgcatc ttatcataaa
    27421 acgaagacag atgccgatct cgctgctata ctctgcgccg ttttcccgtt ctttaacatc
    27481 cagtgagaga gaccgatgca tccgatgctg aacatcgccg tgcgcgcagc gcgcaaggcg
    27541 ggtaatttaa ttgccaaaaa ctatgaaacc ccggacgctg tagaagcgag ccagaaaggc
    27601 agtaacgact tcgtgaccaa cgtagataaa gctgccgaag cggtgattat cgacacgatt
    27661 cgtaaatctt acccacagca caccatcatc accgaagaaa gcggtgaact tgaaggtact
    27721 gatcaggatg ttcaatgggt tatcgatcca ctggatggca ctaccaactt tatcaaacgt
    27781 ctgccgcact tcgcggtatc tatcgccgtt cgtatcaaag gccgcactga agttgctgtg
    27841 gtttacgatc ctatgcgtaa cgaactgttc accgccactc gcggtcaggg cgcacagctg
    27901 aacggctacc gtctgcgcgg cagcaccgct cgcgatctcg acggtactat tctggcgacc
    27961 ggcttcccgt tcaaagcaaa acagtacgcc accacctaca tcaacatcgt cggcaaactg
    28021 ttcaacgaat gtgcagactt ccgtcgtacc ggttctgcgg cgctggatct ggcttacgtt
    28081 gctgcgggtc gcgttgacgg tttctttgaa atcggtctgc gtccgtggga tttcgcggca
    28141 ggcgagctgc tggttcgtga agcgggcggc atcgtcagcg acttcaccgg tggtcataac
    28201 tacatgctga ccggtaacat cgttgctggt aacccacgcg ttgttaaagc catgctggcg
    28261 aacatgcgtg acgagttaag cgacgccctg aagcgttaat gactctggcg ggtgatgtca
    28321 ctcacccgcc ctcgtctttc aggcgctatt ccgaaatact tcctcaccgc tttactttct
    28381 ttcgccactc tcccaccatc tttccccgat taatggatga taaagaagtc gcaaggaaag
    28441 gttatggcgc tgccagtgaa caaacgcgtt ctcaaaattc tgtttattct ctttgttgtt
    28501 gccttctgca tttatttaat accgcgcatc gccatcaatt ttttctatta ccctgacgac
    28561 aaaatctacg gccccgatcc ctggtcagcc gaatcagtag aattcaccgc taaagatggc
    28621 actcgcctac atggttggtt tatcccttct gcaactggcc ccgctgaaaa ggccattgcc
    28681 accattattc atgctcacgg caatgccgga aatatgtccg cccactggcc gctggtcagt
    28741 tggttacccg agcgtaattt caacgttttt atgtttgatt atcgcgggtt tggtaaatca
    28801 aaaggcacgc cgtcccaggc cggattgctg gacgatacgc aaagtgccat caatgtggtg
    28861 cgccatcgca gtgatgtaaa cccacaacgg ctggtgctgt tcgggcagag tattggcggg
    28921 gcgaatattt tggatgttat tggtcagggt gatcgtgaag gcatacgtgc ggtgatcctc
    28981 gactccacat ttgcctctta tgcgaccatc gccaaccaga tgatccccgg cagtggctac
    29041 ttacttgatg agagttacag cggcgaaaat tacatcgcca gcgtcagccc gatcccgctt
    29101 ttactgattc acggtaaagc tgatcacgtt atcccatggc agcacagcga aaagttgtat
    29161 agcctggcaa aagagccaaa acggttgatc ctaatcccgg atggcgaaca cattgatgct
    29221 ttttccgatc gtcacggcga tgtttatcgc gaacagatgg tggattttat ccttagcgcg
    29281 ttgaatccgc agaactaacc catgatcgct agcacgataa tcattcacaa aaccacctta
    29341 agacatgcta atccactggt cagaacagtt taagatgaga aaaattctgt gacgcttgcc
    29401 aacatttctg atgattagca ttcccttcgc catttccctg agcaaacttt agctattctt
    29461 atcaattatg cttatgggag atctacagat gatgcctacg cttgctccac catctgtcct
    29521 ttcggctccc cagcgccgct gccagatctt gctgacgctc tttcagccgg ggttgaccgc
    29581 caccacggca actttcagcg agcttaatgg tgtggatgat gatattgcca gtcttgatat
    29641 cagcgaaaca ggacgggaga tcctgcgcta tcatcaactc acactgacta ctggttatga
    29701 cggtagctac cgggttgaag gtacagtgct taaccaacgt ttgtgtttat ttcactggct
    29761 acgacgtggt ttccgtctgt gtccgtcatt tattaccagc catttcaccc ccgccctgaa
    29821 gagtgaactg aagcggcgcg gaattgcgcg taacttttac gacgatacca atctacaagc
    29881 gttagtgaat ctctgctccc gacggctgca aaaacgcttt gaaacgcgcg atattcattt
    29941 cctgtgtctg tatctgcaat attgtttgct gcaacaccac gctggaatta cgcctcagtt
    30001 taatccgctc caacgtcgct gggcggaatc ctgccttgaa tttcaggtag cgcaggaaat
    30061 tggacgccac tggcagcgtc gggcgctcca gcctgtaccg cctgatgagc cactgtttat
    30121 ggcattactt ttttccatgt tgcgggttcc cgatccactg cgggatgcgc atcagcggga
    30181 cagacaactg cgtaagtcta tcaaacgtct ggtaaaccat tttcgtgagc tgggaaatgt
    30241 tcgtttttat gatgaacagg ggttatgcga tcagctttat acccacctcg cccaggcgtt
    30301 aaatcgcagt ttgtttgcca tcggtattga taacaccctg ccggaagagt tcgcccgcct
    30361 gtatccacgc ctggtgcgca ccacccgcgc ggcgctggcc ggatttgaaa gtgaatacgg
    30421 cgtccatctt tctgatgagg aaagcggtct ggtcgcggtg attttcggtg cctggctaat
    30481 gcaggaaaac gacctgcatg agaaacagat tattctactg accgggaatg atagcgagcg
    30541 agaagcgcag attgagcagc agctacgcga actaacgtta ctgccgctca atattaaaca
    30601 tatgtcggta aaggcatttt tgcagacagg cgctccgcgc agcgcggcac tgattattgc
    30661 tccttatacc atgccgttac cgctcttttc accaccgctg atctatacgg acctgacgtt
    30721 gacaacacat caacaggagc agatccgcaa aatgctcgaa tcagcatgag ggaacaactt
    30781 tcgggcgcaa aaacattgcc ggaagcgcca ccagcgccat cacccagaac acgccgtggc
    30841 ccagatattg atacaggaaa ccggcgaaaa cggtcatgat cgcaatactg ccgcccatcg
    30901 cgacggcaga gtaaaccgcc tgtaaacgga tgacttcgct accctggcga gcggcaatat
    30961 agcgcatggc ggccaggtgg cagaccgtga aggtgccgca atgcagaatt tgcaccacta
    31021 tcaaccacgg catcgccgtc gttgctccca taatgcccca gcgcactacg ccgcaaatcg
    31081 ccgagatcaa cagcatatcg cgtgcactac aacggcggaa aagtttatta ctcagcgcaa
    31141 agataatgac ttccgccacc acgcccagcg accacaaata gcccaccgcc gaggccgagt
    31201 agccagcggc ctgccagtaa atggcgctaa aaccgtaata ggccgcatgc gccccctgca
    31261 ataaacaaac gcaggccaga aagcgccagt tctggcgaac cagcgccaac cacgcagacc
    31321 aaccggtgct ctcctgctgg cggcttgccc cttgtggctg aatcgtcgga cggatgagaa
    31381 agccgagcag catggatgcc acgcccaacg ttaacagcgc gaggatcacc cgataatcaa
    31441 acatagtgac cagtttaccc gtcaacgccg agccaatgac aaacgccacc gagccccaca
    31501 atcgtacttt gccgtaatca agcgggaact gcttttgcca cgtattcgcc agtgcatcgg
    31561 tcaacggtac cagcggtgag aaaaagaggt taaagccaat catcaccagc atcagccacg
    31621 ctacgtgcgc ccccgcccag aaggcgacag caaagagaag tgtcagcagt gccagcacgc
    31681 gcaaggcgga aatcaggcgg gaaggatcgc tgacgcgggg cgcgatgagc aaactcccga
    31741 gaaaacgggc aaccagacct gcccccaata acaggccgat ggtttctggc gttaaaccaa
    31801 tccccttaag ccagacgctc cagaaaggta gaaaaatgcc gtaactaaaa aagtatgtga
    31861 aatagccgag cgccaaccag cgcgtggatt gcaaaaccat gagtccctcc cgtcaggagg
    31921 cgttagtctg gggttaatcg aatgttggtg caagttgaaa aataagaata ttcgagcctg
    31981 cttcccgatg tctgtaacac cagttcacaa gcactaaaaa actgcgatcc gcgacgcatg
    32041 ttgcaaaaat aatgcgtaat tatgccgtta cgcttgccaa acgttcctgc acaatggcga
    32101 tgaaatcgcg taatgccggc ttcatctccc ctttcttcca cgccatcagt aaagcaatgg
    32161 aaggcacatt tccggcaatg ggtcgaaata ccacctggcc ggtattaaaa ttattcatat
    32221 aaccgggtat caaagtgacg cccagcccca tccccaccag attcatggtc accagaatat
    32281 tcgttgccac ctggacgata tttggctggc tgttttcttg cgcaaaccac gctttaacga
    32341 tcggcgcaag cgaaccggaa tacgccggat cagtactgac gaaattcaca ccatccagtt
    32401 gcgcggcagt gatctctttt tcatgtgcta aagggtgatc aaccggtaac accaccacta
    32461 atggttcgtc aaaaagctcc agataatcaa tctccgggct ataaacagga tggcgcatta
    32521 agccgacgtc gagttcacca cggcgaattt tttcttcctg ttccgtggtg attaaactca
    32581 ccagctcaat taaggtgtct ggctgtctga gacgaaacat cggtaatact tttggcagta
    32641 aattcacttc cgccgatggc acaaagccaa tggttaattg tctgtcttcc tgaacaattt
    32701 tccgcgcccg taatttggca ttttccgctt gttcgaggat tgccagcgcg tcctggagaa
    32761 aacattctcc cgccgccgtc aacgcgactt tgcgcttatc cctcaccagc aacggaacac
    32821 cgacacagtt ttcaagatcg cggatctggc tgcttaacga aggctgtgag gtatgcagtt
    32881 tttccgccgc acgggtaaag ttcagtgcct gcgccactgc gacgaaatag cgtaaatgcc
    32941 gtagttccat caccttcccc ttgttatcga aaaaacgtct cagctggtag aaattaacta
    33001 tttcacatat cagcaaccaa ccagcaacat ccttatgaca caaaaataga aggtcaatac
    33061 atcttatctt tcaggattaa aaaatatgac cacaccctca gatttgaaca tttaccaact
    33121 gattgacacc caaaacggtc gggtcactcc gcgtatttat accgacccgg acatttatca
    33181 actggagctt gagcgcattt tcggtcgttg ctggctattt ctcgcccacg aaagccagat
    33241 cccaaaaccc ggtgatttct ttaacaccta catgggagaa gatgcggttg tcgtagtgcg
    33301 tcagaaagac ggcagcatca aggcgtttct caaccaatgc cgccaccggg ccatgcgtgt
    33361 gagttatgca gattgcggca acacgcgcgc ctttacctgc ccgtatcacg gctggtctta
    33421 tggcattaac ggcgagttga tcgatgtacc gctggaacct cgcgcctacc cacaagggtt
    33481 gtgtaaatcc cactggggac taaacgaagt tccttgtgtg gagagttata aagggctgat
    33541 ttttggcaac tgggatacca gcgcaccggg cctgcgtgat taccttggtg acattgcctg
    33601 gtatctggat ggcatgctgg atcgtcgcga aggcggcacc gaaattgtcg gcggcgtaca
    33661 aaagtgggtg atcaactgca actggaaatt cccggcagag cagttcgcca gtgaccagta
    33721 tcatgctctg ttcagccatg cttctgccgt tcaggtatta ggggcgaaag atgatggcag
    33781 cgataagcgt ctcggtgatg gacaaactgc ccgcccggtg tgggaaaccg ccaaagatgc
    33841 gctgcagttt ggtcaggacg gtcacggcag cggtttcttc tttactgaaa aaccggatgc
    33901 taatgtctgg gtcgatggcg cagtttccag ctattaccgc gaaacctatg ccgaagcaga
    33961 acaacgttta ggtgaagttc gcgccctgcg cctggcgggt cataacaata ttttccccac
    34021 gctttcatgg ctcaacggca ctgccacgct ccgcgtctgg catccgcgcg gccctgatca
    34081 agtcgaagtg tgggcgttct gtattactga caaagccgcc tccgatgaag ttaaagccgc
    34141 ttttgaaaac agcgccactc gtgcttttgg tcctgctggt tttctcgagc aggatgactc
    34201 ggagaactgg tgtgaaatcc agaaattgct gaaaggtcac cgcgcccgca acagcaaact
    34261 gtgtctggaa atggggcttg gtcaggaaaa gcgtcgcgac gacggcattc ctggcattac
    34321 taactatatc ttctcagaaa cggccgctcg tggaatgtac caacgctggg ccgatctcct
    34381 gagtagcgaa agctggcagg aagtgctcga taaaaccgcc gcttaccagc aggaggtgat
    34441 gaaatgagtg cgcaagtttc actggagtta catcaccgca ttagccagtt tctctttcac
    34501 gaagccagtc tgctggatga ctggaaattt cgtgactggc tggcacagct cgacgaagag
    34561 attcgttaca ccatgcgcac cacagttaac gcgcaaacac gcgaccgccg caaaggcatt
    34621 cagccgccga cgacctggat tttcaatgac accaaagacc agctggaacg gcgaatcgcc
    34681 cgtctggaaa cgggtatggc ttgggcagaa gagccgccgt cacgcactcg tcacttaatc
    34741 agcaactgcc aggtaagcga aaccgacatc ccaaacgtat ttgccgtgag ggtgaattat
    34801 ctgctttatc gggcacaaaa agagcgcgat gaaacattct atgttggaac gcgtttcgac
    34861 aaagttcgcc gtctggaaga tgacaactgg cgcttgctgg aacgggatat cgtcctggat
    34921 caagcggtaa tcacttccca taacctgagt gtactgttct gatgaatcga atttatgcgt
    34981 gtcccgttgc ggatgtgccg gagggtgagg ctctccggat cgatacctcg cccgtcatcg
    35041 ccctgttcaa cgttggcggc gagttttatg ccattaacga tcgttgcagc catggtaatg
    35101 cgtcaatgtc agaagggtat ctggaagatg acgccacggt ggagtgcccg ctacacgccg
    35161 ccagtttttg cctgaaaacg gggaaagcgt tatgcctgcc cgccaccgat ccgctcacca
    35221 cttatccagt acacgttgaa ggtggtgaca ttttcatcga cttaccggag gcgcagccat
    35281 gagcgatctg cataacgagt ccatttttat taccggcggc ggatcgggat tagggctggc
    35341 gctggtcgag cgatttatcg aagaaggcgc gcaggttgcc acgctggaac tgtcggcggc
    35401 aaaagtcgcc agtctgcgtc agcgatttgg cgaacatatt ctggcggtgg aaggtaacgt
    35461 gacctgttat gccgattatc aacgcgcggt cgatcagatc ctgactcgtt ccggcaagct
    35521 ggattgtttt atcggcaatg caggcatctg ggatcacaat gcctcactgg ttaatactcc
    35581 cgcagagacg ctcgaaaccg gcttccacga gctgtttaac gtcaacgtac tcggttacct
    35641 gctgggcgca aaagcctgcg ctccggcgtt aatcgccagt gaaggcagca tgattttcac
    35701 actgtcaaat gccgcctggt atcctggcgg cggtggcccg ctgtacaccg ccagtaaaca
    35761 tgccgcaacc ggacttattc gccaactggc ttatgaactg gcaccgaaag tgcgggtgaa
    35821 tggcgtcggc ccgtgtggta tggccagcga cctgcgcggc ccacaggcgc tcgggcaaag
    35881 tgaaacctcg ataatgcagt ctctgacgcc ggagaaaatt gccgccattt taccgctgca
    35941 atttttcccg caaccggcgg attttacggg gccgtatgtg atgttggcat cgcggcgcaa
    36001 taatcgcgca ttaagcggtg tgatgatcaa cgctgatgcg ggtttagcga ttcgcggcat
    36061 tcgccacgta gcggctgggc tggatcttta aggaagtacg atgaaagaaa aaacgatcat
    36121 tattgtcggt ggcgggcaag cggcggcaat ggctgcggcc ttgctacgcc agcaagggtt
    36181 cagcggtgag ctgcatctgt tttccgatga gcaacatctt ccttatgaac gccccccgct
    36241 ctcgaaatcc atgttgctgg aagattcccc acagttgcag tctgtgttac ccgctcactg
    36301 gtggcaggaa aacaatgttc atctgcattc cggtgtaacc atcaaaacat tgggccgcga
    36361 cacacaagag ttagtgttag ctaacggcga aagctggcac tgggatcagc tttttatagc
    36421 aaccggcgcg gcagctcgac cgctgccgtt gcttgatgca ctgggagaac gctgctttac
    36481 cctgcgccat gccggtgatg ccgccagact gcgagaagtt ctgcagcccg aacggtcagt
    36541 cgtgattgtc ggtgccggaa ctattggtct ggaactggct gccagcgcca cgcagcgtgg
    36601 atgtaaggtg acagtgattg aactggcggc aagcgtcatg ggccgtaatg caccaccgcc
    36661 cgtgcaacgc tatcttttac cgcgccatca gcaggctggt gtgcgcattc tgctcaataa
    36721 tgccattgaa catgtggtcg atggtgaaaa agtagaactg acgctgcaaa gtggcgaaac
    36781 gcttcgggcc gatgtggtga tttacggtat tggtatcagc gccaacgacc aactggctcg
    36841 cgaggccaac cttgatactg ccaatggtat tgtcattgat gaagcttgcc gcacctgcga
    36901 tcccgcgatc tttgccggtg gcgatgtggc aatcacccgt attgataatg gtgcactaca
    36961 ccgctgcgaa agctgggaaa acgccaataa ccaggcgcaa attgccgctg ccgcaatgtt
    37021 ggggctaccg cttccgcgac tgccgccgcc gtggttctgg agcgatcagt acagtgataa
    37081 cttacagttt attggcgata tgcatggcga tgactggatt tgtcgtggca acccggaaac
    37141 tcagaaggcg atttggttta atctgcaaaa cggcgtgctt atcggtgcgg taacgctgaa
    37201 tcaggggcgt gagattcgct taatccgcaa atggatccag agcggcaaaa cgtttgatgc
    37261 gaaactgctg atagatgagg acatcgcgct taaatcactg taaccaggat aattagcgaa
    37321 tatctcaatg cctggggcgt ggcgaggtgc aagagtgtgt attacgttta aatcacatta
    37381 tcttgcaaag ggattggtta tgaacacact acgttatttt gattttggag ctgcacgccc
    37441 cgttttgtta ttaattgccc gtatcgccgt ggtcttaatt ttcattattt ttggttttcc
    37501 caaaatgatg ggttttgacg gtacggtcca atatatggcc tcgctgggcg cgccaatgcc
    37561 gatgctggca gcgattattg cggtagttat ggaagtgccc gccgcgatat taatcgtgct
    37621 tggctttttc acccgtccgc tggcggtgct gtttattttc tacacgctgg gtacagcggt
    37681 aattggtcac cattactggg atatgaccgg cgatgcggtt gggccaaata tgattaattt
    37741 ctggaaaaat gtcagtatcg ctggcgcatt cctgctgttg gcaattaccg ggccgggggc
    37801 aatttctctc gatcggcgtt aggaaagatg ccggatgcgg cgtgaacgcc ttatccggca
    37861 ttttataaat tacagtaacg caacccgcaa cgacatctct gaagttgttg attcccctgg
    37921 tgcaagcgca atgagatcac ccccttccgg gcgatgatgg tcatccggcg catggctcat
    37981 cggttccaga cagaagaaat caaacgcata tcctttatca aacgccgggt cggaaacaaa
    38041 gatgaaataa catggtgcag gtggcgtcgt ttccatgatg atggcataac cctcctgcgg
    38101 ctgctcgatg cgggcctgac cattccatcc ggcaaagcca ttgtttaccc actggcacgg
    38161 caacggcgcg agctggttga aatccagctc ctgcggtagc tgctcgcaaa actcacccgc
    38221 cagccactgc tcccgctcca gccagtaacc aatcgcctgt gcctgaatcc gcgtttgcgg
    38281 cgacaacgga aaataaggat gccagccggt accaaatggc agcgtctctg ccccttgatt
    38341 ggtgacagag agtgtcaccg tcagcgtatc cgccgttaaa tgaaacgcct ggcttacccg
    38401 atagtgataa acgccgctgc gatgttcata caccagacac aagctatcat cgctgcacga
    38461 ggcacattgc cactgcccca gccagccgtc gccgtgcaga tagtgcgcat cccattcaac
    38521 attcggttgc agttgatact cacgcccctg ccagacaaaa cgattgccgc tcacccggtt
    38581 tgcaaacggc actagcggaa aacaggaggc atcggttgcc acaccgcttt ttttaccagg
    38641 acgtaagagc ggcgtcgtat cacgccagaa accttcgatg acaccacccc ggtcagaaac
    38701 gtccagcttt agcggcccgt gagataaggt atagatggtc atccgctgac tccttaatcc
    38761 gggaagttaa tcacaacttt cccgcattta ccgctcgcca tcagcgcata ggcttctcct
    38821 gcctgttcca gcgtgaagcg atgggtaatg gcattacgcg gccacagttt ccagtccgtt
    38881 aaatcgtggg cgcatttttc catatggaac agactggtca cccaggagcc aataatccgc
    38941 cgttgatgat gcatcagatc agcgctaacc tcgaactcca cttttccggt ttccccgatg
    39001 taaaccaccc gtccccagtc agcagtggat tgcaacgcca gtaagcgacc tgcggcatta
    39061 ccggaacaat cgagcgcaac atccgcgcca ccgtgggtga gttcggcgat aatctgcggc
    39121 agaccttcgg tggttgctaa atagccgtga tccatcaccc ctaactgttt cgccatcgcc
    39181 agacgttccg gcagcatatc aacgccgatg atccgttttg caccgcgacc tttcgccagc
    39241 atcatcgcca tcatgccgac tggccccaaa ccgaccacca gcacgttatc actgccggaa
    39301 acttcgccgc gcaaaattcc ttcatacgct gtaccaacgc cgcaactgat aaacgcacca
    39361 tcttcatagc tcagcgcgtc cggcaggagg atcagatctt tttcttccgc cagcaggtat
    39421 tcagcatggc cgccgtcacg ctgccagccg taagccgctt ttccttcgcc agtacaagag
    39481 ataggaaaac cacgacggca gttcgggcaa aaaccacagc cagaaatgtg atacaccagc
    39541 acgcggtctc cctctttaaa atggcggcag ccttgcccca tcgccacaat ctgcccgcac
    39601 ggttcatgac cgttgataaa gccctggtat aacggtttat cgggcgccgc cgccgtggcg
    39661 cggtgctggt ggtagatata gtggacatcg cttccgcaaa tcccggagga tttcattttg
    39721 atcagtacct ggttaatccc cggcgtcggc accgcaactt cccgcagatc gacggtcgaa
    39781 tttcctggta aataagctgc cagcatcgtt ttcataaatt cctcattaac aatacgatta
    39841 attttcatcc ctgccgcacc cgcgccaggg gcgttaaggt ttagcgtttc gctttactgc
    39901 ttcgctgggt cagcaagata ttcgccagca ccgccaccac gatgatgacg ccgcgtacca
    39961 cctgctggaa aaaggagtta ataccgagca gcaccagacc gttaccgatt agcgtaatca
    40021 ccagcacacc aagcaatgta ccgaacaggg aaccgcgacc gccggaaagt gccgtaccgc
    40081 cgaccacgac cgcggcgatg acgtcaaact ccagaccgtt tgccgcacct gcgttaccag
    40141 aaccgaggcg cgccgccaac aaaatgccgg tcaccgccgc taataatccc gaaagggtaa
    40201 agataagaat gcgcacccga cgaacgttga tgccgcacaa ctgcgccgcc gtagcattac
    40261 cgccaacggc aaaaaccgag cgcccgaagg cggttttgcg gctgatgaac acaaacagcg
    40321 caaacaacac catcatgatc agcgcggata ccggcactcc gagaaattgt ccacccagcc
    40381 agtccagtac ctcgttttcg tcaatcggca ctggcagggc gttggtcata aacagcccca
    40441 ttccgcgcag ggcgctccac agccccagcg tggccacgaa acttggcacg ttaaacacgc
    40501 cgcgcagcac tccggcgagc gttcccatca gcgcgcctaa cagcaacacc agcagacaag
    40561 ctatcgccag cggaacttca aattgcagca aaaatgccag gcacaccgag acaaaagcca
    40621 ccatcggccc aacgctgaca tcaatttccc cggagataat aatcagcgtc atcgcccagg
    40681 cggcgatccc aatggtggcg gcatcgcgca gcacgttcat ctggttattc aatgagataa
    40741 aacccggcgc gttcagagag aagaccagat aaagaatggc gatcaccacc agcaaaccga
    40801 tctcattaat atggcgactg acaaattgtt tgagcgagac gctcttgccc tgcggcaatg
    40861 gtaatgacga agcagacatc ttcaggttcc tcggtaaaat tcatcagtgc acagacagaa
    40921 tggcggacat cagctcatcc acattgaccg gcgagtgaaa ctcctgcgag aacgtaccgt
    40981 gctgtaataa caggatgcgg tcacacacca gcggcaactc ctccacttca ctggagataa
    41041 acaccacgct ttttccttcg gcagccagct cgcggacaat acggtaaatc tgctgtttgg
    41101 cttcgatatc gacgccacgc gttggctcgt cgagcaacaa aatctggctg gcggcataga
    41161 cccaacgacc gatcaccact ttttgctgat tgccaccaga aagcgtgccg atgggcgttt
    41221 cgctactggc ggccttgacc gtcatccgct gcatcacctc ttcggtcagg cggcggatgg
    41281 tggaccattg cagcacaccg ttggcgctga ttttttgccg attggtcagc actgtatttt
    41341 cgtcaacgcc caaccaggga atgatccccg cttctttgcg gttttctggc gtatagccaa
    41401 tgccgcgttt cagcatgtcg ccgtaatcgg ggcgcgtgat tttctcgccg ttgataacaa
    41461 tttcgccctg ttcatacgtc tccagcccaa caatcgcctt cagcaattca ctgcgccctg
    41521 cccccagcag accagcaatg ccgagcactt cgccacgacg tagtgtaaaa ctgatatcct
    41581 ccagcttggg cttatggcgt aacgcacgga cttccagcac ggcttgatcc ataatttcct
    41641 gaggggcaac cggcgcaata tcaacgtgat cgcgcccgag cattagcgac acaatatgat
    41701 gcgtggaggt gttttcgagc atcacatcgc ccgccacctg accatcgcgc ataacggtgg
    41761 cacaggaggc aatgcggcga atttcttcca tccggtggct gacataaatc accgccacgc
    41821 ccagtgccga catctttttc accgcgctga tcaccagttc aacctccgca ctggcaagcg
    41881 agctggtggg ttcatcaaga atgaccacgc gcggctcgcc cttcatcacc cgcgcaattt
    41941 ccaccagctg cttttgcgcc gggcttagtg ttgaaacaag ttgttcagga ctaacgtcaa
    42001 cgcccaacgc ctgcaagcaa cgttgggcgt cctgcgccat ttgcaggtaa tcaatcatgc
    42061 cgttgcggcg gggccactga ccgaggcaga ggttttccgc cactgtcagc ccttccacca
    42121 gacttaactc ctgataaacc gcgcgtaccc ccagttcagc ggcacggcga gtcagcgtag
    42181 cttcgtcacc ttccagtcgc gtctcgccaa tccagatatc accgctatcc ggacgttcgc
    42241 taccggtaag cattcgaatg agggtcgatt ttcccgcacc gtttttgcct aacagcgcac
    42301 gaacttcgcc tttattgagc gtgaagttaa cgttatccag cgcaacgacg ccgggataac
    42361 gcttatttcc tgccaccact tttgctaccg ggactgcctc tgttgctgtg aacataggga
    42421 cctctgcgaa tcagcgatta gggcagacca tcaacgtgcg ttgccagcca ctgtttaccg
    42481 tcttccgttt tggtatagag atcgattggc acctgaatca ctttttcacc gtcggcttgt
    42541 ttattgataa ccttcaaagt ttgcgcgaaa acagcattgc ccattttctt accggaaata
    42601 tccaccaccg ctttcagcac ctgattgttt tccagctcct gagcaatttc agtggtcata
    42661 tccgaaccga aaacagcaat ttttccggcc tgattttgat tacgtaccgc ttttaccgcg
    42721 ccgagtgtcg caccgcccga ttcccccata atggcgttga gatccggcgt ggagataatt
    42781 agtttttcac caacggaaat cgctttatct aaaacagtcc cttcctgatt agcgactatt
    42841 tgcgcgccgg gaacgcggga ttttaatacc tcttcaaatc ctttacgtcg ctgcacacaa
    42901 acttcaaagg cttcgcaatt gatgacggca attttcggct ggtcaatttt attggcaata
    42961 aaataatcgg cggcagcgtt acccagtttt ttaccaaatt ccagcggatc gccgaccaga
    43021 tacgccgaga catatttatc gacacctttt tgattaatac aggtgttgta gcaaatcacc
    43081 ggaatacccg cttcactggc gcgacgaacg gtacgactac tgccattttc agacactgcc
    43141 gataaaataa tggcatcgac attacgcgcc acgagggtat caacaaaggt actttctttc
    43201 gaaatatcac cctgggcgtt agtttcaatt aactgtacct gtactgaaga atcttttgcc
    43261 gcatcctgaa cgccctgacg cacgccagcg taatatccct gggtatcaag gtatattgca
    43321 ccaatggtca tttctttatc cgcagccctg gcaaataaag cgctacctaa tagcgtagcc
    43381 attaataata gattacgggt tgttctcatt tttttaggca tagtgcctcc tgtagggttt
    43441 ttatttacaa cggcttattc taattatttt gtgatgagcg gcagcgcgtg ccgctcaaat
    43501 attacagtta atagggttta gtgaacgcgg ttaaaaataa aaggcatcac agtggtgaat
    43561 aatgccgctt tcggccaggc cggattaatc gccgtcaatt cagcgcgagc agattcatag
    43621 tgcgcaacct ccccgagtcc cgcggacgcc agcatcgcca ccatcaggca tttttcttta
    43681 tgctgctgtt gtaaatcgcc atacagcgac aacaggtcag gttgtgaaac agcaaagaaa
    43741 tccgcctcaa tactggtttt cgccatctct tgcgcccact gtttcatttc actaaacagt
    43801 tgctgtgcgg tttgctgttc acccagcagt cgcagcgcca ttccttgcca gaagagataa
    43861 tcaaccggct gatcgttgta ataactgtga atgttaatgg tgcgatcgcc ggtcgccgcc
    43921 agacgtaaac aacgcgtcgc ttcagtttca tcgccctgcg cgttagcgca tatcgcctgc
    43981 cagaaccaga tgtcgttatc agtttgcccg ggtaaacggc cttcgcttaa attttccgga
    44041 taatgcagcg cggcatgaag cagttcgctg gcctgctgcg gctctctggc atcaaggtgc
    44101 tgccaggcgc gtaatagctg attgaggata aactgactgg tgaccttccc ttccccgcct
    44161 tcccacgggt ggaatttgcg cgtggcgaga atgtccgccg ctttgtctgc ctgacccgtg
    44221 agatgccaca aattgagcag ttctgcggtc atgtcgtcgc gtttcagcgc aatttccaga
    44281 ttattttcca gacgcgccag tcgtttctcc ggtgtggctc cgcttaactt atcaagcaaa
    44341 tcccgttcga aaagcagacg tgcatcctgc ggcgcaagct gataagcatt atcaagataa
    44401 cgcgcggcca gctcataatc gtgttgctta ttccacgcat ggatcgctaa cccgcgccag
    44461 ccgtcggcaa actccggcga catctctacg caacgttgcc ataaagtaat ggctttgttg
    44521 tagctgcgct tgttgtagta gaagcaggcc agtaaatgac gggcaaacca gcactcttca
    44581 atactctcca gcgccgccac ttcttccagc gtattcggga aacggacaaa ctgtgggaag
    44641 acatcaatgg cttttgtgac cagttcgccg cgttcagctt tcggcagcaa gctggcttgc
    44701 aagtaaagcg gcagcgtgcg ctggcagtcc agtgcgttca gcatctctgc cgccagggtg
    44761 ggcattcccc agttaatcag ttgcccggcg gtcatcaggg cgttaacgtc gcgtccctga
    44821 cacagaccgc gccactgcgt gaaggctgat tcgctacgac catcgaacca gttcagccac
    44881 cacagagtgg cgttcagcgg ataatcgcgc agcagtttct cgcgctgcaa acgcgcgttg
    44941 tcctgacgac cacttaacac cagcagcaga ttatgcaggc aaagcacttc ctgattggtt
    45001 gggcaggcgc gaagactttg ttggcaaaaa tccagaccag cgtcgaagtg accattacgc
    45061 gccgccagcc gtgccagacc ataatagcca cctgctttac tgttgccgct ccagaccgcc
    45121 cgccagaaat cctcttcggc ttgttgatat tgcccctgac gttcgtaagc actggcgcga
    45181 atcaaactcg cctgtccgca ctgcggattt ttgtttagcg catgtgcgcg tttcagcgcc
    45241 tgagtggcat acgccaccgc ttgcgggaaa tctgctcggt tatattccag catcgccagc
    45301 gccaggttac agcgataatc cagcggatcc agcgccacgc cgcgcaggta gtaatcgaac
    45361 ggtgaacggc tggcgtgatg atattgctcc agatgctgac cgataaacca ggcttcatct
    45421 gtactggtaa tgtcttgtgc tgccagtggc gctttggcga cgtccggcaa cggcaactct
    45481 tgcggctgat gttcctgata actcagtgtg atattgccgt cggcatcgga gagttcaatg
    45541 gtcagccttt caggattgat accgtgcaac acgccctgga tggcggtcgc tggtgtcagg
    45601 gccacggcat catcgagcaa cgcgttgcat ttgccgattt cgcggatcgc cagacggtat
    45661 cctttcaacg gagagatggc atacagcccc cactcaatcc cccgctcgct acgctggagt
    45721 ttaatcaccg catcgcggga ggcgttttgc accatgccca aagaatggta aggcaggaaa
    45781 tactgctcaa aacgcttctc ttcgtaagca tcaagccagg taaaatcggg ctggttatcg
    45841 gcaaaaatac cagtcatcag ttcgatatac gggccgttat tgtcggtcag actcttatcc
    45901 cacgcctggc caaattcact gtgtccccag ctccactgtt ttttacctgg cgcaatatgg
    45961 tggttggcaa cgtgcagcaa accgccatct tcatcgtgac accacgcgcc aacaaaatcg
    46021 tactgtgatt tttcagccat atatgaggtt ggaacgggca catttttata gcgagaaatg
    46081 tccactccgg cggagtagtc cactttgtag taagtgccgg tggcgatggg gaaagcggag
    46141 acggcccgtt tgccgtgatc aaacaccgcc gttacatccg gcgggaagac gctctgatgc
    46201 ccttcacccc ctttcactgc cgggttggcc caccacaaga aatgacgcgg cgtggcgttg
    46261 ccgttataga cgcggctggc aatttccagt gccgcccggt cagggcgcag ggtgaaacct
    46321 gtcatcacct gtaaaccgtg catcggctcc gtttcgccga cccacacggt ttgtgcgccg
    46381 tcttcatggg cttcgagggt gaaatcgacg ggcataaagg tggtcgggcg atggtgttgc
    46441 ggccagttaa actcaatccc gccggagatc cacggcccca gcagccccac cagcgccggt
    46501 ttaatgactt cattatgata gacaaaatcg cgctgtttca ctttatccca cgcgcgatgc
    46561 acccgaccgc ccagctccgg caggatcatc actttgatgt agtcgttttc cagccacacc
    46621 gcctgccagg atttcagggt tttctgctcg ctcagcgtat cggtcacgcc gtagggataa
    46681 accgcgccgg acgatccctg ataaacgcga ttttccagga acatgggatg tatatcctgc
    46741 ggcccggttt cataggtcgg gatctcaacg cgctcttgcc acacttttac tggagtcatg
    46801 gtgccctcaa tattaacaag acatactgaa ttaaaagatt tgtggcagtg tattgaacaa
    46861 tctggcaatg ttttcgcgga ataatcacgc aattaactaa acaaggttta gtgaagatga
    46921 gagcctgcat taataatcaa cagattcgcc accataacaa atgcgtgatt ctggaactgc
    46981 tgtaccggca aaagcgcgcc aataaatcaa cgctggcccg gctggcgcaa atttcgattc
    47041 cggcggtcag taatattttg caggaactgg aaagcgaaaa acgggtggtg aatatcgacg
    47101 atgaaagcca gacgcgcggg catagtagcg gtacatggct gattgcgccg gaaggtgact
    47161 ggacgttgtg cctgaacgtg acgcccacca gtattgagtg tcaggttgct aatgcttgtt
    47221 taagtccgaa aggtgaattt gagtatttac agattgatgc accgacgccg caggcgctgc
    47281 tgtccgaaat cgaaaaatgc tggcatcgcc accgtaaatt gtggccggac cgtaccatca
    47341 acctggcgct ggcaatccac ggtcaggttg atccggtgac cggcgtgtcg caaaccatgc
    47401 cgcaagcgcc gtggacaacg ccggttgagg taaaatatct gctggaagag aagctcggca
    47461 ttcgggtgat ggtcgataat gactgcgtga tgctggcgct ggcggagaaa tggcaaaata
    47521 attcgcaggg ccgggatttc tgcgtgatca acgttgatta cggcattggc tcgtcgttcg
    47581 tgattaacga gcaaatttat cgcggcagtt tgtatggtag cggacagatt ggtcacacca
    47641 tcgttaatcc ggatggcgtc gtctgcgact gcggacgtta tggctgcctg gaaaccgtcg
    47701 cctcgttaag cgcattaaaa aaacaggcgc gggtatggct aaaatcacaa ccggttaata
    47761 ctcagcttga tcctgaaaaa ctgactaccg cgcagttaat cgctgcctgg cagagtggag
    47821 aaccgtggat caccagttgg gttgatcgct ctgccaacgc cattggtttg agtctgtata
    47881 acttcctcaa catcctcaat attaatcaga tttggttgta cggtcgcagt tgtgcctttg
    47941 gtgagaactg gcttaatacc attattcgcc agacgggatt taacccgttc gaccgcgacg
    48001 aaggaccgag cgtgaaagcg acacagattg gctttgggca attaagccgc gcacaacagg
    48061 tactgggaat tggctatttg tatgttgagg cgcagttacg gcagatttga tggcgcgata
    48121 acgtagaaag gcttcccgaa ggaagccttt ttcaacagat taaccgcttc gcttatgcgt
    48181 aaaccgggta acgtgcacag atgtcgagaa ctttaccttt gatgcgctcg ataacggctt
    48241 catcattgat gctgtccagc acgtcacaca tccagcccgc cagttctttc gcttctgctt
    48301 ctttgaagcc gcgacgcgta atcgccggag tacccacgcg aataccggag gtcacaaacg
    48361 ggctcttcgg atcgttcggt acactgtttt tgttgacagt gatgttagca cggcccagag
    48421 cggcgtctgc ttctttaccg gtcaggtttt tatcaaccag atcaaccagg aacaggtggt
    48481 tatcagtacc gccggaaacc actttgtagc cgcgttcgag gaacacttct accatcgctt
    48541 tggcgttttt cgcgacctgc tgctggtaag ttttgaactc aggttccatc gcttctttca
    48601 gagcaaccgc tttaccggcg attacgtgca tcaacggacc gccctgacca ccagggaaaa
    48661 cggcagagtt cagttttttg tacagctctt cgctaccacc tttcgccagg atcaggccgc
    48721 cgcgcggacc cgccagggtt ttgtgagtgg tggtagtaac aacgtgagca tgaggaaccg
    48781 ggttcgggta gacgccagca gcaaccaggc ccgcaacgtg cgccatatca acgaacaggt
    48841 aagcaccgat gctgtcagcg atttcacgca ttttcgccca gtccaccacg ccggaatatg
    48901 cagagaagcc accgataatc attttcggtt tgtgttcttt ggcttgtttt tccagatcgg
    48961 cgtagtcgat atgaccgcta gcatcgatac cgtaaggaac gatgttgtac agtttaccgg
    49021 agaagttaac cggagaaccg tgagtcaggt gaccgccatg cgccagattc atacccagaa
    49081 cggtatcgcc tggttccagc agcgcggtgt agaccgcaaa gttagcctgg gagccggagt
    49141 gcggctggac gttagcgtag tcagcgccga acagttcttt cgcacgatcg atcgccagtt
    49201 gttcaacgat atcaacatac tcgcaaccgc cgtagtagcg tttgcccgga taaccttcag
    49261 catatttgtt ggtcagctga gaaccctgcg cctgcattac gcgcgggctg gtgtagtttt
    49321 cggaggcgat cagttcgatg tgctcttcct gacgtacttt ttcctgctcc atagcctgcc
    49381 acagttcggc atcataatcg gcaatgttca tttcacgctt taacatccgc atctcctgac
    49441 tcagctaaca ataaaatttt tggcctttat aggcggtcct gttggacaac ggcgaacagt
    49501 ataaccgaat cattgtgcga taacaggtct tgacaaagga atttacgcaa acgattacct
    49561 tcaggctgcg caaggctttg gagaataaag agcttgcaac cggaaacgga tttcttttca
    49621 ggtttgtgat gcaaattttt cacttcatca cattctttct gaaaaacacc aaagaaccat
    49681 ttacattgca gggctatttt ttataagatg catttgagat acatcaatta agatgcaaaa
    49741 aaaggaagac catatgcttg acgctcaaac catcgctaca gtaaaagcca ccatcccttt
    49801 actggtggaa acggggccaa agttaaccgc ccatttctac gaccgtatgt ttactcataa
    49861 cccagaactc aaagaaattt ttaacatgag taaccagcgc aatggcgatc aacgtgaagc
    49921 cctgtttaac gctattgccg cctacgccag taatattgaa aacctgcctg cgctgctgcc
    49981 agcggtagaa aaaatcgcgc agaagcacac cagcttccag atcaaaccgg aacagtacaa
    50041 catcgtcggc gaacacctgt tggcgacgct ggacgaaatg ttcagcccgg gccaggaagt
    50101 gctggacgca tggggtaaag cctatggcgt actggctaat gtatttatca atcgcgaggc
    50161 ggaaatctat aacgaaaacg ccagcaaagc cggtggctgg gaaggtactc gcgatttccg
    50221 cattgtggct aaaacaccgc gcagcgcact tatcaccagc ttcgaactgg agccggtcga
    50281 cggtggcgca gtggcagaat accgtccggg gcaatatctc ggcgtctggc tgaagccgga
    50341 aggtttcccg catcaggaaa ttcgtcagta ctctttgact cgcaaaccgg atggcaaagg
    50401 ctatcgtatt gcggtgaaac gcgaagaggg tgggcaggta tccaactggc tgcacaatca
    50461 cgccaatgtt ggcgatgtcg tgaaactggt cgctccggcg ggtgatttct ttatggatgt
    50521 gacggatgac acgccggtaa cactgatctc tgcgggtgta gggcaaacgc caatgctggc
    50581 aatgctcgac acgctggcaa aagcaggcca cacagcacaa gtgaactggc tccatgcggc
    50641 agaaaatggc gatgttcacg cctttgctga tgaagttaag gaactggggc tgtcactgcc
    50701 gcagtttacc gcgcacacct ggtatcgtca gccgagcgaa gccgatcgcg ttaaaggtca
    50761 gtttgatagc gaaggtctga tggatttgag caaactggaa ggtgcgttca gcgatccgac
    50821 aatgcagttc tatctctgcg gcccggttgg cttcatgcag tttgccgcga aacagttagt
    50881 ggatctgggc gtgaagcagg aaaacattca ttacgaatgc tttggtccgc ataaggtgct
    50941 gtgatttgat gttgccggat ggaaacatcc ggcaaccctt gacgcggttt aaattgccgc
    51001 gtcgtcctct tcaccggtac ggatgcgaat gacccgtgcc acgtcgaaga cgaagatttt
    51061 accgtcaccg attttgccgg tttgcgccgt gcgaataatg gtatcaacac aggtatcgac
    51121 aatgtcgtcc ggtacgacaa tctcaatttt cactttcggc agaaaatcca ccatatactc
    51181 cgcgccgcgg tacagctcgg tatggccttt ctggcggcca aaacctttca cctcagtgac
    51241 cgtcatgccg gtaatgccga cttcagccag tgcttcgcga acatcgtcca gcttgaaggg
    51301 tttaataatc gcatcaatct ttttcatgct attccttgaa aaggtcgcct gtcttttgat
    51361 ctgctaaacg taacacataa cgccaattca ttccttgaaa tcgtttgcat ccagctcgtg
    51421 tcgggaaagc agtttataaa attctgtccg gttgcgcccc gccattctcg ccgcgtgggt
    51481 gacgttgcct ttggtgattt gtagcagctt acgcaaatag ttgagttcaa actgattacg
    51541 tgcctcaaca aaggttggca gcgccgtgtt ttcgccctcc agcgcctgct ctactaatgc
    51601 atcgctaatc accggagatg aggtcagcgc cacgcactgt tcaatcacgt tgaccaactg
    51661 gcgcacgttg cccggccagc tcgcggtcat caggcgtttc atcgcatcgg tggagaacgc
    51721 gcggacaaac ggtttatgtc gctctgccgc ctggcgcaac aggtgatttg ccagtagcgg
    51781 aatgtcttct gtgcgctctg ccagagccgg aattttcagg ctgacaacgt tgaggcggta
    51841 atagagatct tcacggaatt ccccgcgcgc catcgctttt ggcaggtcgc ggtgagtggc
    51901 ggaaataatc cgcacgtcga tatcaatatc gcggttactg cccagcgggc gcactttacg
    51961 ctcctgcaac acgcgcagca gtttaacctg caacggtgcg ggcatatcgc caatctcatc
    52021 gagaaatagc gtaccgcctt ccgccgcctg gaataaacct tcgcgattgc tgacagcgcc
    52081 agtaaacgca ccacgcgcat gaccaaacag ctcggactcc agtaattgct cgggcaatgc
    52141 gccacagtta atagcaataa acggcttgct gttgcgcggg ctggcgttgt ggatagcctg
    52201 ggcgaaaatc tctttcccgg tgccgctctg gccattaatc aaaacgctga cgtctgattg
    52261 cgccaccagc cgcgcctgtt ccagcaaacg cagcatcagc gggctgcggg tgacaatcgc
    52321 ctcgcgccag cgttcgtcgg tggctggcgc ggattgttcc agcgcatcat caattgcctg
    52381 atatagcgcg tctttatcga caggcttggt gaggaaactg aacactccct gctgtgttgc
    52441 agcaacggca tcgggaatag aaccatgtgc ggtaagaata attaccggca ttcccggctg
    52501 cactttctgg atttcagcaa acagctgcat accatccatt tcatccatcc gcaggtcgct
    52561 gataactaaa tctacttttt cgcgattcag tacccgtaat ccttcagcac cactttccgc
    52621 cgtgaccacg ctgtagcctt cgctggtcag gcgcaggcca agcagtttca gcaatcccgg
    52681 atcgtcatcg accaataata aatgcgcagg tttatggctc atcaggagtg acctcatggg
    52741 tggatggcgc gggcttttca ctctcgtgcg gcgtatccgg cgagaaattt ccagccggtt
    52801 tgcgggtcga gagctggcgt tcaatatcag tcaggttttc cagcttgcgg gtggtgagtt
    52861 ccagttgctg ctgtagaacg tgatgttgct ggcgcaatgt atccagctcg ctgtcgctgg
    52921 attgctggag tttgccgtag cgttggcgct cttccgccag ttgcagttgc agcgcctgac
    52981 cgtcgcgcca gagttgatac agagggcgaa cctgtgccgg gatctcggta cttagcgcat
    53041 caatgcgcgc gaccagctgg cggcgctcat acggcgtaat tttggcgtcg gcgagcaaaa
    53101 tcccttgttt aaaggtattt tgccagttgc cgtcgtcata ttggcgggct tgctgacgcg
    53161 actgcgcagg cattaaacga tcagcacaat ccatcgcccg cagccagtaa agcggattgg
    53221 tttcggttga tttaccttgc agcgcccaga tgtcgctaca ttcagtagaa agatagtcag
    53281 ccagttgata aaccggaatt ttttcttctg ctggcgtatc aatggctggc ttattgtgac
    53341 tctgcacgca acccagcaat gccagacatg gcagccctgc cagccacagc cgtcggggca
    53401 ataatcgttg aaaaatgtgt cgcatattca ccagacttaa agcctatccc agtgggcgta
    53461 attgttgcag gcagtttgga catggacagc gcggagaaac cagagcgtac atgtcgtacg
    53521 tgaagatttc gagcactgcc cggggccgaa ctgacaaata aaatagcctg atgggatggg
    53581 ctgttagcat tatttcgtgt ttttcgacga cggtaattca atgcggaaac aaacgtcttg
    53641 cccgctctcg tcgaccagat acagttcccc ttgcatacgg cgaatacaat ccctggcaat
    53701 gcttaatccc agaccgctgc ccttcaccgc cccttttcgc tggtggcttc cttgaaaaaa
    53761 gggttcgaag atcatggcgc gttcctcttg cggaatgggt gtgcctgtat tgatgacatc
    53821 aatataaacc cgcgcaccat gtaaactgct gcgaaggcaa atgttaccgg attcagtccc
    53881 gtagtacaca gcattggagt aaagattatc cagtacgctc atcagcagca ttggctccgc
    53941 caggcaagct gttgcttgga gatcgacgtc ggtatgcatc attttagccc gtgcgggcag
    54001 gctatgagca gaaaccactg tctccaccag cggtgctaac tcaacattct ccagttccac
    54061 cgcactgtcc gcctgtttac ggttgtaatc aagcagttgt tcgatcagct tttgcaaatt
    54121 gcggctgctg ctatcaagaa tgctcaccac ctctttttgc tctggcgtaa gcggcccgac
    54181 aacctgatca gccagtaatt cagtgccctc gcgcatactc gccagtggcg tttttaattc
    54241 atgagataaa tgtcttaaaa attgatggcg ttgggattcc agccatgaca ggcgctcact
    54301 taaccaaaga atacgttgcc caaccgagcg taactcgctc ggtccactga acgagacgct
    54361 attgcccaga gaacgccctt cccccagacg gttgatcatg cgctcgatat ttttcaccgg
    54421 cccgataatc atccgcgtga aaagcagtac catcaccaga ctcaccagaa atagcaccag
    54481 cgattgccaa ccaaaatatt gcccacgttc ggcgatttca cgctgaagtt gctgcccacg
    54541 agagaacacc actgtgcgcg tggcctgtac catttcggta ttggcactgg caaaggcttc
    54601 aagacgtgcg gcggcggcat catcgggacc gctgttgtta cactgaagtt gagccagatt
    54661 gttcaaatcc tgacgtaatg cctggtagag tttatcgtcc ggcagcacgc ctgcgtgggc
    54721 atcgagcatt tcgctgtaac gcttgcgctg gctttgataa accttcgcca gggttgggtc
    54781 gtccagcacg caatactgac ggtaactacg ctccatctcc agcgccgcgt tggtcatcgc
    54841 ttcactgcgc cgggcatcaa taagcgtagt gcggttaacc agcgccgcct gatcgctaag
    54901 cgcattcagg ctttgccagg cttgccatgc cagcaccaac aggggcagca gaatcagcaa
    54961 aaatgccagc attaccagtt gtcgtaatga gcggggaaaa acgggccagc gtttcaaggt
    55021 gttactctcg tcagacgcga atagcctgat gctaaccgag gggaagttca gatacaacaa
    55081 agccgggaat tacccggctt tgttatggaa taaggcggtg cctaactcga cgtttcgccc
    55141 gatggttgat atagctacgc tgatatcaga agttggacgg caggcacctt gttgtgcgtc
    55201 attcgtattt tatgtagcac gtcccgaagg ggctgacata agtcggtgaa tgagccactg
    55261 gttactatta tgcagtaact gtgccaataa agaaaatagt ttggtaacgt actgattata
    55321 cgctgctttg agggatttat ggcttcttcg ctatttgaat gtaaatcagc cactgtgtcg
    55381 ctaaaaagag acaacttaag ataaacttat tagatactat taaaatcaat cagttaagtg
    55441 tcgccagaaa gcgacacggt aaaccaccca ttgtcgtgat ttacagacac aaaaaagctc
    55501 ccgaagttgg gagcttataa tagcggttgg tgcttaatgc cggatgcggc tgcaaaacca
    55561 atgggttgac ggcttacccc aactgcttac gtgcattgcg gaaaatacgc atccacggcc
    55621 catcctcgcc ccagttttcc ggatgccagg agttgctgac agtacggaaa actcgttccg
    55681 ggtgcggcat cataatggtg actcgaccac tttcagtcgt gaccgccgta ataccgttcg
    55741 gtgaaccgtt cgggttagcc gggtaggttt cagtgacttt accgaagtta tcgacatagc
    55801 gcagtgccac cagccctttg ctttccagcg ccgccagatg cgccgcatca cgcacttcca
    55861 cgcgcccttc accgtgagag acagcaatcg gcatctgcga gcccaccatc ccctgcaaca
    55921 gcagcgacgg gctttgggtt acttctacca gactgaaacg cgcttcaaag cgatcggagg
    55981 tgttacgcac aaaacgtggc cacaactcgc tacccgggat cagttctcgc agattagaca
    56041 tcatctggca accgttacat acccccagcg ccagcgtttg cggacgatgg aagaaggttg
    56101 caaactcatc gcgtacacgg tcattgaaca ggatagactt cgcccaacct tcaccagcgc
    56161 ccagcacatc accgtaggag aaaccaccgc acgcgaccag ggcgtggaaa ccctccaggc
    56221 ccgtgcgtcc agccagcaga tcgctcatat gcacgtcgat agcatcgaag cctgcacggt
    56281 ggaaagctgc cgccatttca acatgcgagt tcacgccctg ctcacgcagc acggcaactt
    56341 tcgggcgcgc gccagtggca ataaacggtg ctgccacatc ttcgttgata tcaaacgaca
    56401 gtttcacatt caggcccgga tcggcgtcgt ttgatttcgc ctggtgctcc tgatcggcac
    56461 attcagggtt gtcacgcagg cgctgcatct gccaggtagt ttctgcccac cagacacgca
    56521 acgtagtgcg gctttcgctg aatacagtct gcccgttggc ggtaatcaca aaacggtcac
    56581 cggaaaccgc ctgccctaca taatggacgc aatccgcaag cccatgctgt gccagtacgg
    56641 actcgaccgc ttcacggtca gcggcacgaa cctgaatcac cgcacccagt tcttcgttaa
    56701 acaacgcagc caggcgatcg tcgcccagag aggcgatatc cgcgtcaatc ccgcaatgac
    56761 cggcaaacgc catttccgcc agcgtcacca gcaggccacc gtcagagcgg tcgtggtacg
    56821 cgagcagttt acgctgtgca accagtgcct gaatcgcgtc atagaaacct ttcagttggg
    56881 caacgtcgcg tacatctgcc ggtttgtcgc caagctgacg ataaacctgc gccagcgccg
    56941 ttgcgcccag cgcgttattg cctttgccca gatcaatcag cagtaatgca ttatcttcgg
    57001 tagaaagctg cggcgtgatg gtgtgacgca catcttccac gcgggcaaat gcagaaatca
    57061 ccagcgacaa cggcgacgtc atttcgcgct cttcgttacc ttcctgccag cgggttttca
    57121 tcgacataga gtctttaccc accgggatcg tcaggcccag cgccggacaa agttcttcgc
    57181 ccacagcttt aacggcttca tacaggcccg catcttcacc agggtggcct gccgccgcca
    57241 tccagttggc ggaaagttta atgcgtttga tatcgccaat ctgtgttgcg gcaatattgg
    57301 ttaacgcttc accgaccgcc agacgggcag aagcggcgaa atccagcagc gcaaccggcg
    57361 cacgctcgcc aatcgccatc gcttcaccgt agtagctatc gaggctggcg gtagtgaccg
    57421 cgcagttagc gaccggcacc tgccacggac ccaccatctg atcgcgcgct accatgccgg
    57481 ttacgctgcg gtcaccaatg gtcactagga aagttttctc cgccacggtc ggcagatgca
    57541 gcacgcgttt caccgcgtca gcaatggtaa tcccttcacg aaccagcgca tcacccttcg
    57601 ctttcagcgt ttgtacttcg cgggtcatct tcggcgtttt accaagcagg acgtccagcg
    57661 gcagatcgat cggctgatta tcaaaatgac gatcgtgcag agaaagatgc agttcttcgg
    57721 tcgcctcacc aatcaccgcg tagggtgcgc gctcacgctt acacagctcg tcaaacagcg
    57781 gtaactgatc ggcagcaacc gccagcacgt aacgttcctg ggattcgtta caccagattt
    57841 ccagcgggct catgcccggt tcgtcgctca ggatatcgcg cagttcgaat ttaccgccgc
    57901 gcccgccgtc gctcaccagt tccggcatgg cgttagaaag accgccagcg ccgacgtcgt
    57961 ggataaacag gattgggttg gcatcaccaa gctgccagca acggtcgatc acttcctggc
    58021 agcgacgctc catctccggg ttgtcgcgct gtacggaagc aaagtcgagg tcggcatcag
    58081 actgaccaga cgccatggaa gacgccgcgc cgccgccaag accgatgttc attgccgggc
    58141 cgccgagaac gaccagcttc gcaccaacgt tgatctcgcc tttttgtacg tgatcggcgc
    58201 gaatgttgcc gatcccgccc gccagcatga tcggtttgtg ataaccgcgc agctcttcgc
    58261 cattgtggct gttcactttt tcttcataag tacggaagta gccgttcagt gccggacgac
    58321 caaattcgtt gttaaatgcc gcgccaccca atgggccttc ggtcatgatg tccagcgcgg
    58381 tgacaatgcg ctcaggctta ccgaaatctt cttcccacgg ttgttcgaag ccaggaattc
    58441 gcaggttgga aacggagaaa ccgaccagac cagccttcgg cttcgcgccg cgcccggtgg
    58501 caccttcatc gcggatttca ccgccggaac cggtcgccgc gcccggccac ggagaaatcg
    58561 ccgtcgggtg gttgtgagtt tcgactttca tcagaatatg cgccggttcc tgatggaaat
    58621 cgtagcggcc cgtttcgtgg tcagcaaagt agcggcccac ttcagaacct tccattacgg
    58681 cggcgttatc tttataagca gagagaacgt ggtctggcgt ggtttcgaaa gtatttttga
    58741 tcatcttgaa cagcgatttc ggctgctgtt caccgtcgat aatccagtcg gcgttaaaaa
    58801 ttttgtggcg acagtgctca gagttcgcct gagcaaacat atacagttcg atgtcgttcg
    58861 ggttacgacc cagctttgtg aacgcatcct gcagatagtc aatttcatct tccgccagcg
    58921 ccagaccaag acgcaggtta gcgtcgatta gcgcctgacg gccctgcccc agcaaatcaa
    58981 tgctggtgac cggggttggc tgatgatgag caaacaagtg ttctgcatca tctaaagcaa
    59041 aaaagaccgt ttccatcatg cggtcatgca gttcagcggt aacctgctgc cattgttcat
    59101 tggtcagcgt accggcttct acatagtaag caacgccgcg ctcaaggcgg tttacctgtt
    59161 gtagaccgca attatgggcg atatcggtcg ctttcgaaga ccagggagag atggtgccag
    59221 gacgcggggt caccagcagg agtttgcctt gcggggcgtg gctggcgagt gccgggccat
    59281 atttcagcag gcgttcaagt tgtgcgtgct catcatcgtt taacggcgca ttgaggtcag
    59341 caaaatggac atactcggcg taaatagtgt gaaccgggag cctggcagcc tgaaaacgtg
    59401 ccagcagttt gttgattcgg aatgccgaca atgcaggcga accacgcaga atttccatca
    59461 taagtctctc gtcttctaag ctttcggtgt acccaagggg gggaaacggg cgtcattata
    59521 aagaatctga tgcgctgacg aaaccgtttg cgtggaaata aaatcaccat cgtgaattag
    59581 caacgcgtgc cgccaatggc tgtaataagt tgccaactgg cgcaggttta cgcaaaatgc
    59641 cgctcattta tgagtaaacc tttcactatt attacgtttt ttcaagctgg gacgcgcacg
    59701 acacagagaa ttaactaatt gaaaaaatta aagattaatt atctgttcat cggcattctg
    59761 gcactgctgc tcgcggtcgc tctctggcca tccattccct ggtttggtaa agccgacaac
    59821 cgtatcgccg ccattcaagc gcggggagag ttgcgtgtga gcaccattca tactcccctg
    59881 acgtataacg aaatcaacgg gaaacctgtt ggcctggatt acgaactggc gaaacagttt
    59941 gccgattacc tcggcgtaaa actgaaagtg accgtgcggc agaatatcag ccagttattt
    60001 gacgacctcg ataatggcaa tgccgacctg ctggcggcag gactggttta taacagtgag
    60061 cgggtaaaaa attatcagcc tggccctacc tattattccg tgtcacaaca actggtttat
    60121 aaagtgggtc agtatcgccc acgtacgctg ggcaacctga cggcggagca actcaccgtt
    60181 gcaccgggtc atgtggtggt taacgatctc cagaccctga aagaaacaaa attcccggaa
    60241 ttaagctgga aggttgacga caaaaaaggc tctgcggaat taatggaaga tgtcatcgaa
    60301 ggaaaactcg attacaccat tgctgattct gttgccatta gcctgtttca gcgcgttcat
    60361 ccggaactcg ccgtagcgct cgacattacc gatgaacaac cggtgacctg gtttagcccg
    60421 ttagatggcg ataataccct ttccgccgct ctgctcgatt tcttcaatga gatgaatgaa
    60481 gacggtacgc tggcacgcat tgaagagaaa tacctggggc atggcgatga ttttgattac
    60541 gtcgatacgc gcacattttt acgcgccgtc gatgcggtac tgccgcagtt aaagcccctg
    60601 tttgagaaat acgccgaaga aattgactgg cgtttgctgg ccgctattgc ttatcaagaa
    60661 tcgcactggg atgctcaggc cacttcaccg acgggtgtgc gcggcatgat gatgttaacc
    60721 aaaaacaccg cgcaaagcct cggcattacg gatcgtaccg atgccgaaca gagcatcagc
    60781 ggcggcgtgc gttatttgca ggatatgatg agtaaagtgc cggaaagtgt gccggagaac
    60841 gaacggatct ggtttgccct tgccgcgtac aatatgggct atgcgcatat gctggatgcc
    60901 cgcgccctga cggcaaaaac caaaggtaat cctgacagtt gggctgacgt aaaacagcgt
    60961 ctgcctttac ttagccagaa accctattac agcaagctga cttacggcta cgctcgtggg
    61021 catgaagcct acgcttatgt cgaaaatatt cgtaagtatc agattagcct ggtgggttat
    61081 ctgcaggaga aagagaagca ggctacagaa gcggcgatgc aactggcgca ggattatccg
    61141 gcggtatcgc ctacggagct gggcaaagag aaatttcctt ttctctcgtt tctttcccag
    61201 tcgtcatcaa actatttgac ccactctccc tctctgctgt tttccagaaa agggagtgaa
    61261 gagaaacaaa attaatctgt cgaggattgc gcttttttct gcgctttaat ttcctggcgg
    61321 cgcatgcgaa agaagtcact gagcaacgcc gcgcactcat ccgccagtat tccttcggta
    61381 atttccaccc ggtgattcat acccggatga tgcagcacat ccattaaaga tcccgcagcg
    61441 ccagttttcg cgtcacgcgc accaaagacc acgcgaccaa tgcgactgtg gatcattgct
    61501 ccggcacaca ttacacaggg ttcaagcgtg acatacagcg tggcgtcgat cagtcgataa
    61561 ttttgcatca ccagaccgcc ctgccgcagg gccatgattt ctgcatgtgc ggtgggatca
    61621 tgtcgaccaa tcggacggtt ccagccttcg ccaataacac gattgttatg cactaatacc
    61681 gcgccgaccg gcacttcccg ctcatcccag gcacgctttg ccagcgtcag cgcgtgacgc
    61741 atccagtatt cgtggctaaa ttcgacttca gacaaaaaga aaactccggt tataaaagcg
    61801 cggcgcatta tacacggacg ctatgcttta ctctattcca gttgctggag ttcaccgcgc
    61861 ggggttactc gccaacgatg ctgacagaaa taaagcagcg gattgtcctg attactgtcg
    61921 ctatagccac tgtacagccg cagcggcgtg ccgattttgc gctccagttg tgcgaccttt
    61981 tcatgtccca gacaacgcat cgtcaatacc caaccaccat agccacgctg aatttggctg
    62041 gcgataagat taacccgcgg cagccagggc gtatcgaaat aaaccgcttc aaccagcggc
    62101 tgcggagagc cggtaatcaa ccagatatca gcatcggaac ttaacaggta ggtggttaat
    62161 cgctcctgaa ccagcggaaa ggcggtaaca ttgtcacgaa accagcgtac gaaatcggcc
    62221 tgcaacgcct gcaaacgcgc ttcattgtga ccaaaagtgc atccccacag aagcagactc
    62281 atcggccagc gtgccgcacg accttttatc aataacgcaa tggctatgat cggcaacaac
    62341 ggcaggacaa gtaacgcatt cagcggttgg cgacgcagta aataacgcag aaaactgccg
    62401 aacatatcct gctgatgcaa tgttccatct aagtcaaaaa acaccacacg acgctcgtga
    62461 gttgccaaac catactcctc tggactgaaa catcctgatt aattttcgct actacatagc
    62521 ctaacagata gatcatcact tttccggcaa caatcgaccg catcatggct aactggaatt
    62581 tttaattcat ggcaattagc ggcaatggaa tataaaattc actcgcgtgt gtctcatatt
    62641 gcctgttgtc gccactattt catcgcgagg aaagatatga acggcttact tcgtatccgt
    62701 cagcgttacc aggggcttgc ccaaagtgat aaaaaactgg cggattatct gctgctacaa
    62761 cctgatacgg cgcgccattt aagctctcag caactggcca acgaagccgg agtcagtcag
    62821 tccagcgtcg tgaagttcgc gcaaaaactc ggctataaag gttttccggc acttaagttg
    62881 gcgttgagtg aagcgctggc aagccagccg gaatctccct ccgtgcccat tcataaccaa
    62941 atccgcggtg atgatccgtt acggctggtc ggcgaaaaac tgattaaaga aaatactgcc
    63001 gctatgtacg caacgttaaa cgttaatagt gaagagaaac tgcatgaatg cgtggcaatg
    63061 ttgcgctctg cgcggcggat aattctgacc ggtattggcg cttcgggtct ggtggcgcaa
    63121 aactttgcct ggaagctgat gaagattggc ttcaatgctg ccgcagtgcg cgatatgcat
    63181 gcgctactcg ctacagtaca ggcgtcgtcc cctgacgatc tgttattagc catttcctac
    63241 accggtgtac gacgcgagtt aaacctggcg gcagatgaga tgctgcgagt gggcggaaaa
    63301 gtgctggcga ttaccggctt tactcccaat gccctgcaac agcgtgcttc tcattgcctg
    63361 tataccattg ccgaagaaca agcgacaaac agtgcttcaa tctctgcttg tcacgcgcag
    63421 ggaatgttaa cggatttgct gttcattgcg ctgattcagc aggatctgga actggctcca
    63481 gaacgtattc gtcacagtga agcgctggtg aaaaaactgg tctgagtaaa gaatgcgcgt
    63541 ataatgcccg cccggtttgt gttgttttga gagtttcctt atggcgctgt taatcactaa
    63601 aaaatgcatc aattgtgata tgtgtgaacc cgaatgcccg aatgaggcga tttcaatggg
    63661 cgatcatatc tacgagatta acagcgataa gtgtaccgaa tgcgtagggc actacgagac
    63721 accaacctgc cagaaggtgt gcccgatccc caatactatt gtgaaggatc cgcaacatgt
    63781 cgagacggaa gaacagttgt gggataaatt tgtgcttatg catcacgcgg ataaaattta
    63841 atattctacc ctggatgtag agtgttaatt attttataga gaaaccttaa cgctattata
    63901 tttatttgat tgatgacatt agcataatta ttcactaagt taatttatat agtatctgcc
    63961 cagacactta tttatagtta ttaaaggcgc gtccgactgg ttcaccggac gcaccttaag
    64021 tacgtttcct tgtgttataa gaacagaagg atcagctgta aaacagcaat gatgattttg
    64081 ataatccgtt taatcaggta tcggcaatca gtcattcgtt tttccttaaa caaggaatgc
    64141 agccgttcag attagccctt acatcacccc aaaactgaac gtgcgagtta ttggaatcga
    64201 gtgctgcact ccacaccaga gctttgacga caccactcgt ttcaatgggg gaattcagtg
    64261 gcatggtgta aagcacagca aaattgccaa taacgaaacc gattttaatg tacttatcaa
    64321 aaagcggtca cgttaatctt tttatcaatg tcgatttctt cgtaaaggct cgcgtttttt
    64381 atgtcagaga ttgcaagttg cttgtacagg ataacgaaat tgatccataa tcatttctgt
    64441 tggaatcgtg tgctgcacaa aattaaagtt gtaaagtgaa tacccccgtt ccttaccagt
    64501 tcgggggttt tactttataa agagaacggt atttttttaa ctttcaataa ttaccgtggc
    64561 acaagcatag tggcgttcat ctgccagcgt tacatgcata tttacaacgc cgagcttttc
    64621 cgccagtttt aatgcctcac cccatagccg tagccgtggt ttgccgagct catcattgaa
    64681 tacttcaaat tgattaaacg ccagaccatt gcggatcccg gtgccaaacg cttttgccgc
    64741 cgcttctttc acagcaaaac gcttcgccag aaaacgcacc ggctggtggt gcgttttcca
    64801 gattgcccat tcgttatcgc ttaatacgcg gcgtgccagg cgctcaccgg atcgggcgat
    64861 caccgcttct atgcgagcga tctccacaat atccgtacct aaacctaata ttgccattag
    64921 ccacgcgctt ccagcatcag acgcttcatt tctgccaccg catctttcag tccggtcatc
    64981 actgcacgac caataatggc atgaccgata ttcagttcat gcatctcagg gatggcggca
    65041 atagctttca cgttgtgata ggtcagaccg tgaccagcgt taactttcag accgagacta
    65101 gcggcaaagg tcgcggcttt ggcgatacgc gccagctctt gcgcctgttc ggcgtcagtt
    65161 ttggcatcag catagcaacc ggtgtggatc tcgataaacg gtgcgccaac ctctgccgca
    65221 gctttgatct gctcttcatc ggcgtcgata aacagagaaa cctggatccc ggcctccgcc
    65281 agacgtttac aggcatcgcg cattttgtca cgctgccctg cgacgtccag gccgccttcg
    65341 gttgttactt cctgacgctt ttccggtacc agacagcaaa aatgtggctt cgtctcaacg
    65401 gcgatcgcca gcatctcttc ggtcaccgcc atctccagat tcatgcgggt atccagcgtc
    65461 tgacgcagga tgcgcacatc gcggtcagta atgtggcggc gatcttcacg taaatgcacg
    65521 gtaatgccgt ccgctcccgc ctgctcagca ataaacgccg cctgcaccgg atccgggtaa
    65581 gcagtaccgc gtgcgttacg cagcgttgca atatggtcaa tgttgacgcc taacagtaat
    65641 tcagccatga caatcctcat cattcataat gtgttttcac cgttcgctta ggcataaact
    65701 gccggaacag ttccctgctc tttaatggtt taccgccaag atacggctta agcgccatgc
    65761 gggtaaagcg tttcgcggcg cgcagtgtgt ctgcgtcagg aaattcccgc gcgtttaacg
    65821 cttttaactg ccttccggtg aacgttttat tgtcgataac gacgcttgcg ataaaccctt
    65881 tttcttcgcg ataacgatac gtcatggtgt catctaccgg ctcgccgcta cccgcacaat
    65941 gggtaaaatt gacgccatag cccagatgcc cgagcagtgc cagttcaaag cgacgcagcg
    66001 cgggttctgg cgtaccagtg gcacctgcaa gtgactgaat gcagtgcaag taatcgaaaa
    66061 agagttcaga gaagcgcgtc tcgtattcca gtacgcggga gagaagttcg ttgatgtaca
    66121 gaccgctgta aagcgtgata ccgcttaatg gcagcgccag cgagacagct tcagcactgc
    66181 gcagtgtttt gacttcacca cgcccaccga agcgaagcaa gagaggggtg aaaggttgta
    66241 atgcaccttt cagagtagag cgtttagagc gtgcgccttt ggcaaccaga cgcacgcgac
    66301 ccgattcctc cgtgaagacg tccagcatca ggctggtttc gctccacggg cgactatgca
    66361 ggacaaatgc gcgctgccag ccttccatcg gagttactct taaagatcgt caacataacc
    66421 gagactgcgc agtgcgcgtt cgtcgtcggc ccaaccggat ttcactttta cccacagctc
    66481 aaggtgaaca ggcgcttcaa acatttcctg catgtcttta cgcgcttcaa tcccgatggt
    66541 tttgatcttg gcccctttgt tgccaataac catcttcttc tgcccttcac gctcaacgag
    66601 aatcaaaccg ttgatgtcat aaccgccgcg ttcgttagag acgaaacgtt cgatctccac
    66661 ggtcacggag tacggcagtt cagcgccgag gaaacgcatc agtttttcgc ggatgatttc
    66721 agacgccata aaacgctgtg agcgatcggt gatgtaatct tccgggaagt gatgggtcgc
    66781 ttcaggcaga tgcttacgca cgattgctgc gatagtgtcg acattcagcc cggtttcggc
    66841 agagatcggc acaatatcca ggaagttcat ctggcttgcc aggaactgta agtgcggcag
    66901 cagatcggct ttttcctgca cgttatccac tttgttcacc gcgaggatta ccggcgcttt
    66961 gccatcgcgc agtttgttga gcaccatttc atcgtccggc gtccagcggg tgccttctac
    67021 gacaaaaatc accagctcaa cgtcgccgat agagctgctc gccgctttgt tcatcaggcg
    67081 gttaatggcg cgtttttctt ccatatgcag gcctggggta tcaacgtaga tcgcctgata
    67141 cgcgccttca gtatggatcc ccacaatgcg gtgacgagtt gtttgcgcct tgcgggaggt
    67201 gatggagatc ttctgcccca gcagtttgtt caacaatgtg gatttgccaa cgttcggacg
    67261 tccgacgata gcaataaatc cgcagtaact tttatcgatg ctcattccag ctccagtttt
    67321 ttcaacgcct gttcggcggc agcctgctca gccttacgac ggcttgaacc tgtgccaacc
    67381 accggttcac tcaggccgct gacctggcag tggatagtaa attcctgatc gtgcgcttcg
    67441 ccacgtacct ggactaccag ataagttggc agcggcagat ggcgaccctg caaatattcc
    67501 tgcagacgcg ttttcggatc tttttgttta tcgcctgggc taatttcgtc cagacgagtt
    67561 tgataccagt tgaggattaa tttctcgacg gtttgaatat cactgtcgag gaatacgcca
    67621 ccaattaatg cctcgacggt gtcagcgaga attgactcac gacgaaatcc accgctttta
    67681 agttcacctg gccctaaacg taagcactca cctaactcaa attcgcgcgc cagttcagcc
    67741 agcgtattac cacggaccag cgtggcgcgc atccggctca tatcgccttc atccacacga
    67801 gggaaacggt gataaagcgc attggcgata acgtagctca gaatagagtc acctaaaaat
    67861 tctaaacgct cgttatgttt actgctggca ctacgatgag ttaatgcctg ctgcaacagt
    67921 tcctgatgat taaaagtgta gcccagcttc cgttgaagcc gattaattac gatggggttc
    67981 atgcgatacc aataaataaa tgcgtcaaca attcagcaca cgaaacagac ctgatataca
    68041 tggctcggct aactgcttca ctgcagtttc agtttaccgg tatatgggga ccaacgctgt
    68101 ttcgtgtgcc gtggcaacct ggaggtgcca accttaaact tcgggggaat attctataca
    68161 caacgacggg ggatgtcgtt agccacggga gatttatctc ataaataatt cacgttgtcg
    68221 ccataacggc gacaacgtga acgaagatgg ctattaatgg ataccgccaa tgcgacttaa
    68281 gcgcacacca gtcggccatt cgccttcttg cttatcgaag ctcatccaga tagccgttgc
    68341 ccgaccgacc agattcgctt caggcacaaa gccccagtaa cggctgtccg cgctgttgtc
    68401 gcggttgtcg cccatcatga agtattgtcc cggcggaaca atccaggttg ccagttgttg
    68461 ccctggctgc tggtaataca tccccacctg gtcctgcgca atcggcactg tcagaatgcg
    68521 gtgcgtcaca tcacccaatg tctctttacg ctcggaaaga cgaattccat tttctttggt
    68581 ttcgtttttc ggcacttcaa agaatccgct ggtcgcttcc ccaccattac ggcgtgagaa
    68641 ggtctgaacg aaatcgctcg gttccacgtt ggagtaagtg accggtagcg cgttttcgca
    68701 cgcctggccg gaactgcatc ccggttgaat cgtcagctct tttgagaccg gatcataagt
    68761 gactttatcg cccggcaaac ccaccgcgcg cttgatgtaa tcaagctttg gatcttccgg
    68821 atatttaaag accacgatat cgccgcgttt cggatgaccg gtttcgatca gcgttttctg
    68881 gtagatagga tctttaatgc cataagcaaa cttctctacc agaataaaat caccgattaa
    68941 cagagtcggc atcatcgaac ctgacgggat ctggaacggt tcataaataa acgaacgcac
    69001 aatcaatacg atagccagca ccggaaaaac agaagcaccg gtttccagcc agccaggctt
    69061 cggcgcaact ttcttcagcg ttgctttatc cagtgagtca ccggcagccg cctgcgccgc
    69121 tgcctgacgt tcccgccgtt taggtgcgaa aaagaattta tccacgcacc ataaaatgcc
    69181 cgtcaccagt gtggcaatca ccagaatcag ggcaaacata ttcgccatgc caactcctaa
    69241 gggttatttg ttgtctttgc cgacgtgcag aatggcgagg aacgcttcct gcggcagctc
    69301 gacgttaccg atctgcttca tgcgtttctt accttctttc tgcttctgca gcagcttttt
    69361 cttacggctg atatcgccgc cataacattt agccagtacg tttttacgca gctgtttcac
    69421 ggtggagcgc gcaatgatgt gcgtaccaat cgctgcctga atggcgatat caaactgctg
    69481 gcgtgggatc agatctttca tcttctccac cagctcgcga ccgcggtttt gcgaattatc
    69541 acggtgggtg atcaacgcca gcgcatcaac acgttcaccg ttgattaata cgtctacacg
    69601 taccatgtcg gacgcctgga agcgtttgaa gttgtaatcc agagacgcat aaccacgcga
    69661 ggtagatttc aggcgatcga agaagtcgag caccacttcc gccatcggga tctcgtacgt
    69721 cagcgccacc tgattaccgt ggtaaaccat attggtctgc acgccgcgtt tttctacgca
    69781 cagcgtaata acgttgccga gatatgcctg cggcagcagc atgtgacact ctgcaatcgg
    69841 ctcgcgcagt tcgtaaatgt tatttaccgc tggcagcttg gatgggctgt cgacgtagat
    69901 aacttctctt gacgtggttt caacttcata cactacggtc ggcgcagtgg tgatcagatc
    69961 cagatcgtat tcacgttcca gacgttcctg gatgatctcc atgtgcagca ggccgaggaa
    70021 gccgcagcgg aaaccaaagc ccagcgcgct ggagctttcc ggctcataga acagtgaagc
    70081 atcgttcagg ctgagtttgc ccagcgcgtc acggaaggct tcatagtcgt cggaacttac
    70141 cgggaacaga ccggcgtata cctgcggttt gactttctta aagccaggca gcgccttttc
    70201 tgccggatta cgcgccagcg ttaaggtatc gcctactgga gcgccgtgga tatctttaat
    70261 cgcacatacc agccagccta cttcgccgca tttcagttca gtacggtcaa cctgcttcgg
    70321 cgtgaagatg cccagacggt cggcgttata ggtctgcccg gtactcatga ctttcacttt
    70381 gtcgccctta cgcagggtgc cgtttttaat acggataagt gaaacaacgc ccaggtagtt
    70441 gtcgaaccag gagtcgataa tgagtgcctg caacgggcct tccggatcgc cttccggcgg
    70501 cggaatgtcg cgcaccagac gttcgagaac gtcctgtacg ccaacgccgg ttttcgctga
    70561 acagcgcacc gcgtcggtgg cgtcgatgcc gacgatatct tcaatttctt ccgccacgcg
    70621 ttcaggatcg gctgccggca ggtcaatctt gttcagtacc ggcaccactt cgagatccat
    70681 ttccatggcg gtgtagcagt ttgccagggt ttgcgcttct acgccctgcc cggcgtcgac
    70741 caccagcaat gcaccttcac aggcagccag cgaacgagaa acttcatagg agaagtctac
    70801 gtggcccggg gtgtcgataa agttaagctg ataggtttcg ccgtcagacg ctttgtagtc
    70861 cagcgtcacg ctttgcgctt tgatggtaat gccacgctca cgctcaagat ccatggaatc
    70921 gagaacctgc gcctccattt cacggtcaga caggccaccg cagatctgga taatacggtc
    70981 agacagcgtc gatttaccgt ggtcaatgtg agctatgatc gaaaagttac gtatattctt
    71041 cataaagtat gattattgtg ccttaatgcc cggttaacca ggcttttaga agtcgctgtt
    71101 ctgagcttaa cgtctgtatt aatagaaacg ccgcattcta cactacaaca ttgaggcgag
    71161 gaaatgttca taccgtatgg attgtggtat ctggaaacgt cctcgcattt gttatgcaaa
    71221 atgcaacaaa gccagtgaaa tcactggctc gcgtcttccg aagatgtttc aaatcgcaca
    71281 aggccaggcg gcaaggccac gcttaagatg atcggttgcc attcagaccg ggcagcaaac
    71341 tttcgagagc agccacgtgc aatcaggaat ccaccaacgc caccgagaat cgcaccgcat
    71401 aatgctgcaa tatctgaagc aaagagtagc tgaaatagtg aagcgatgag gaatagtccc
    71461 accaacggcg acatataaac cagtaatgcg gagctaagca ggctgccttc ggcgattcct
    71521 aattctactt tttgccccgg cactaacggc tcatcacagg gtacgacaat ggtatgcgtg
    71581 gtttgcgggc caagtttatt caacacgcgg ctaccgcaac cggcacgtga agcgcagctg
    71641 ctgcatgagg ctttaacatc acaactaacc agcgcctgcc cgttttccca ggagacgacg
    71701 gtagcccact ctttgatcat tgcgctgccc cgaacttaat attctcggca atgcgtttcg
    71761 ccgtttgcgg cggcagttca ccgacaatgg tgatttcggc gttatcacgt acgcttgtac
    71821 tgacggttct gcgcccggtg cgcaacatct gatcggtgct cgatggcgta gcgcggttaa
    71881 cgtttaccga gaagctgaat aatccgtcgg aatagagacg tgattcgata ggcatgttgt
    71941 ccatcgtcgg tagcggacgt cgactactgg aaacttcgct aaaaccctgt ggcaaccagg
    72001 ttggcgtcca gctgaattta gctttttcac ctacaggaac agaaagcaac ggcggcaaat
    72061 ttgccttcgc cagcgtctgc atactgctgc tgatatcctg attgacatta aaagcaatca
    72121 cgcgaaattg ttccagcgtt tcaccatcgc gatcaaggag atcaacccgc atcggtaatt
    72181 tcgattcggt gtccatccac acgatgtagc tgtagcgtgt accatctcgg gcaaccacac
    72241 gaatgacttc gcaaagacga tcagcaatac gcgtgcgccc gacggagata aagtcgtagt
    72301 aaggagaaag gcgtttgaaa tcggtataga tgagcgatgg tagagaatca acaatgtaat
    72361 cgccattaag cgtgaacggt tcaagtcccg gttcaaaata gctgatttca ttgccgcgct
    72421 gtaccacttc ccggcgcggg ccatccattt gcaacaattg tgcaagagga cggttatcga
    72481 ggcgtgcatg tcgataacgc agagactcaa caccctgttt attgatgctg atgaatgaca
    72541 gctcgtaatt cagtgactga ctggccaggt tcatctgctg taataacgcc ccggacgcgg
    72601 gagtggccga ggcgttagca gagaataaca ggctacctgt cactaatgac atggcaaacc
    72661 aaagttgctt cattactgcg attgcgttcc taaagtttga attcctggca cctgtacagc
    72721 ggcttgctgt gtttgtgcct gctcaaactg aagctgttca gagtggagtc gacgttgcag
    72781 ttcataatcc tgcaacatgg cattaatgcg acgacgctgc tcctgtacct gctgctgttg
    72841 accattgttt gcggtcgctt cagaaggtac tcccagactt accggactgg ctttccccat
    72901 catcggcaga gtattaaata ccggtgtttc gggctgctgg gacgtttcag attgtccatt
    72961 atagtgctgg acaccaacga taactgcaag cgatacacat gccgctacgc ccatttgggt
    73021 aagctgtgcc gcccatggac gtattttctg ccagaatggc attttctgcc attgatgcgg
    73081 cgcaggctgg gcttccggga tcaacgtcgc cggttgacgt actggctctt cttcaatggc
    73141 ggccatcacg cgtgaagaga tatcgaaatg gagcacctcg ggagtatcac cccgcattga
    73201 gtcacggatt aagtgatagc tttcccaggt tttctgcatt tctgggttat gagccagttc
    73261 gttaagcagc tcactatcca gcgtttcgcc atccattaaa gcggaaagtt gttctttctg
    73321 catgcctaat acccttatcc agtatcccgc tatcgtcaac gcctgataag cggttgaact
    73381 ttgttatcaa tagcttccct cgctcggaag atacgtgaac gcaccgtacc taccggacaa
    73441 tccatgatag cggctatctc ttcatagctc aggccatcca gctcccgcaa ggttattgcc
    73501 atgcgtaaat cttccgggag ggactcaata gttcggaaaa ctatctgtct cagttcttct
    73561 gacaacatta agttctcagg gttcgaaatt tctttcaacg cgccgccact ttcgaagttt
    73621 tcagcttcaa tggcatccac atcactggaa ggtggacgac gcccctgagc aaccaggtaa
    73681 tttttcgctg tatttacagc aatccgatac agccatgtat aaaaagcgct atctccccgg
    73741 aacgaatcca gcgcacgata ggctttaata aaagcttctt gtaccacatc gggaacatca
    73801 cccgacggca catagcggga aaccagactc gccactttat gctgatagcg cactaccagt
    73861 aagttaaagg ctttctgatc tcccttctgg acccgttcaa ccaggacctg gtccgttaac
    73921 tgctcgctca tccgaggtaa agtctcccca aaccaaattt ccacgcgcta tcgaaacgcc
    73981 actccattag ctgcaatttg agcaagcaaa gggttagagt gtctcgtttt tgtaaagttc
    74041 cgtaacgcat ctgtttttgt ttgtcatgct gtagacggat cattatctat cattataagt
    74101 ctacagaatc tgaacatcgc attatctgtg tagaaatgcc catttaactg cctgaagagt
    74161 aacccaacgg cctttttatt tcaccaccta atcctccacc agccagtaac ttctcttttt
    74221 ctcgccgccc tgcgtcagcg tgtttagcaa ctgtaacaaa tattaaaata gcaggtgttt
    74281 attcgcacaa catgatgcta tgctgaccaa acaatgttta gtaaattaaa caaagaaaat
    74341 gaatactctc cctgaacatt catgtgacgt gttgattatt ggtagcggcg cagccggact
    74401 ttcactggcg ctacgcctgg ctgaccagca tcaggtcatc gttctaagta aaggcccggt
    74461 aacggaaggt tcaacatttt atgcccaggg cggtattgcc gccgtgtttg atgaaactga
    74521 cagcattgac tcgcatgtgg aagacacatt gattgccggg gctggtattt gcgatcgtca
    74581 tgcagttgaa tttgtcgcca gcaatgcacg atcctgtgtg caatggctaa tcgaccaggg
    74641 ggtgttgttt gatacccaca ttcaaccgaa tggcgcagaa agctaccatc tgacccgtga
    74701 aggtggacat agtcaccgtc gaattcttca tgccgccgac gccaccggta gagaagtaga
    74761 aaccacgctg gtgagcaagg cgctgaacca tccgaatatt cgcgtgctgg agcgcagcaa
    74821 cgcggttgat ctgattgttt ctgacaaaat tggcctgccg ggcacgcgac gggttgttgg
    74881 cgcgtgggta tggaaccgaa ataaagaaac ggtggaaacc tgccacgcaa aagcagtggt
    74941 actggcaacc ggcggtgcgt cgaaggttta tcagtacacc accaatccgg atatttcttc
    75001 tggcgatggc attgctatgg cgtggcgcgc aggctgccgg gttgccaatc tcgaatttaa
    75061 tcagttccac cctaccgcgc tgtatcaccc acaggcacgc aatttcctgt taacggaagc
    75121 actgcgcggc gaaggcgctt atctcaagcg cccggatggt acgcgtttta tgcccgattt
    75181 tgatgatcgc ggcgaactgg ccccgcgcga tattgtcgcc cgcgctattg accatgaaat
    75241 gaaacgcctc ggcgcggatt gtatgttcct cgacatcagc cataagcccg ccgattttat
    75301 tcgccagcat ttcccgatga tttatgaaaa attgctcggg ctggggattg atctcacaca
    75361 agaaccagta ccgattgtgc ctgctgcaca ctatacctgc ggtggtgtga tggtggatga
    75421 tcatgggcgt acggacgtcg agggtttgta tgccattggc gaagtgagtt ataccggctt
    75481 acacggcgct aaccgcatgg cctctaactc cttgctggag tgtctggtct atggctggtc
    75541 ggcggcagaa gatatcacca gatgtatgcc ttatgcccac ggcgtcagta cgttaccgcc
    75601 gtgggatgaa agccgcgttg agaaccctga tgaacgggtg gtgattcaac ataactggca
    75661 cgagctacgt ctgtttatgt gggattacgt cggcattgtg cgcacaacga agcgcctgga
    75721 acgcgccctg cggcggataa ccatgctcca gcaagaaata gacgaatatt acgcccattt
    75781 ccgcgtctca aataatttgc tggagctgcg taatctggta caggttgccg agttgattgt
    75841 tcgctgtgca atgatgcgta aagagagtcg ggggttgcat ttcacgctgg attatccgga
    75901 actgctcacc cattccggtc cgtcgatcct ttcccccggc aatcattaca taaacagata
    75961 aaaagcctgg gtcagcgccg tatacgcttc ggaatagttc tggtctggcc cacgaatgac
    76021 taagcgatcg ctaaagcatt ctcccgcctg cggggagaat gccagcagca cccgatgcgg
    76081 cagtcgcgct tcgttttccg ccacatccgt ccgcaaacgt aaatgccagc ccatacttaa
    76141 tgccagttca gtaaaaccat taccaatctg ctctggcagc accacgcaga aaaatccctc
    76201 ttcggtaatg cactccgccg cacaggtcag caacgatggg tgatcaagcg tagtggtata
    76261 gcgagcctgt tcccgttgag gtgtcgcgca ctctactccc tgctgatagt aaggtgggtt
    76321 actgatgatt aaatcgaagc gtactgtctg ctgtgtgatc cactgctgaa tatccgccgt
    76381 atggacgtta atccgctctg cccacgggga ctggttgata ttttcctgcg cctgcgcggc
    76441 agcttcactt tccagttcaa ctgcatcaat cattacgctg tcatcggttc gctgcgccag
    76501 cattaatgcc agcaacccgc tacccgcgcc gatatcaagg caacgtttta ccccagccac
    76561 cggtgcccat gcgcccagca aaatgccatc cgttcccact ttcatcgcac aacgatcgtg
    76621 agcaacaaaa aactgtttaa aagtaaatcc attacgacga agcacggatg tagactgtga
    76681 catgaaaata aaaccttgca ggaaaaacgg cgatagcacc gggtgagaac aatacccgag
    76741 aagcgatatc catacaaaca gatgaagatt gcagccgtaa cgtctataat cagcgcccca
    76801 cacagaggta gaacatgact gtaacgactt tttccgaact tgaactcgac gaaagcctgc
    76861 tggaagccct ccaggataaa ggtttcactc gcccgaccgc cattcaggct gccgccattc
    76921 cgcctgcgct cgatggccgt gatgtactcg gttctgcgcc gacaggcacc ggtaaaacgg
    76981 cggcgtatct gctgccagcg ttgcagcacc tgctcgattt cccacgtaag aaatccggtc
    77041 cgccgcgtat tttgatcctc accccaactc gcgagctggc gatgcaggtg gccgatcatg
    77101 cccgcgaact ggcgaaacat acgcatctgg atattgccac catcaccggc ggcgtagcct
    77161 atatgaacca cgcggaagtg ttcagcgaaa atcaggacat cgtggtcgcc acgaccggac
    77221 gtctgctgca atacataaaa gaagagaact tcgattgccg cgcggttgaa acgctgatcc
    77281 tcgacgaagc agaccgtatg ctggatatgg gcttcgccca ggatatcgaa catattgctg
    77341 gcgaaacgcg ctggcgtaaa cagaccctgc tcttttcggc aactctggaa ggcgatgcga
    77401 ttcaggactt tgccgagcgt ctgctggaag atccggtgga ggtttctgcc aatccgtcca
    77461 cccgtgagcg caaaaaaatt catcagtggt attaccgcgc cgatgatctt gagcataaaa
    77521 ccgcgttgct ggtgcatctg ttaaaacagc cggaagcgac ccgctcaatt gtgtttgtgc
    77581 gtaagcgtga gcgtgtgcat gagctggcaa actggctgcg cgaagcaggc atcaacaact
    77641 gctatctcga aggtgagatg gtacaaggca agcgtaacga agcgattaaa cgcttgaccg
    77701 aaggccgcgt aaacgtactg gttgctaccg atgttgccgc gcgcggtatc gacattcctg
    77761 acgtcagcca cgtctttaac ttcgatatgc cgcgcagtgg cgatacttat ttgcaccgta
    77821 tcggacgtac cgcgcgcgcc ggtcgtaaag gcaccgcgat ttcgctggtg gaagcccatg
    77881 accatctgct gctgggcaaa gttggccgtt atattgaaga gccaattaaa gctcgcgtaa
    77941 ttgatgagtt acgtccgaaa acgcgtgcgc caagcgagaa gcagaccggc aagccatcga
    78001 agaaagtgtt ggctaaacgt gccgagaaga aaaaagctaa agagaaagag aagccgcggg
    78061 tgaaaaaacg ccatcgcgac actaaaaata ttggtaagcg ccgtaaacca agcggaacgg
    78121 gcgtgccacc gcaaacgaca gaagagtaat ctcaatgccg ggtttaagcc cggcattgtt
    78181 tttatcccgg caaaaaacac tcttcaacac actgaataaa agtgtgcatc gccgggctaa
    78241 ccgcttttcc ggcatggtga gcgcacatgg ccgtaatggt ttgcgactgt tcgccaaaag
    78301 gcaattcaat taattctccg cactccaact cttttaccac cgcaaaacgc ggcaggtaac
    78361 tgactccgat atttgccgcg acacaacgct tgatgctttc gatacttata agctcaatgg
    78421 tgttttccac cgtgatccgc cgctggcgca gcgtgctctc aaatatctgc cggaagacac
    78481 attgtggttc gttgataata aagctacagg cgttatgtct tcccggctca gtaaaatcga
    78541 catctgcaat ttgcggtgaa gccaccagca ccagcggttg ttcacccaac tctcgtcggt
    78601 tcaaagcatc atcattccct acgcgataaa aaaccccgac atcggcctca tcattcagta
    78661 gcgcatcacg gatcacgtaa cagttcagcg actgcaacga taaacgcact tttggagcgc
    78721 gttgccgaaa acgctgcaac acctgcggca ttcgataaga gagcaacgtt tcgcctgaaa
    78781 taacgcgtag ttccccgtcc ggatccgact cttttttggc agcctcacga agggtatcca
    78841 tcactctggt gagttcataa atgtgcggca gcagcttttt tccttcgcgg gtaaggcaca
    78901 ttcgtcgacc aattttctca aataactgga ctgagaactc ctgctcaagc tgctgaatat
    78961 gaaaagtcac cgtcgattgt gtgcagcaca atttttgcga agctcgcaaa aaggaaccct
    79021 cttccaccac ggttttaagt gtaataaaac ggcgcagatc cataacccca aacctatcga
    79081 aaatatcgaa tttagaatgt aaaaacattc atttttttaa atgttccgtg tcgggtactg
    79141 tctaccaaaa cagaggagat aacaagtgac accgaccctt ttaagtgctt tttggactta
    79201 caccctgatt accgccatga cgccaggacc gaacaatatt ctcgccctta gctcagccac
    79261 aacgcacgga tttcgtcaga gtactcgcgt gctggcaggg atgagtctgg gatttttaat
    79321 cgtcatgttg ctatgcgcgg gaatttcgtt ttcactggct gttatcgacc cggcagcagt
    79381 gcatctgttg agttgggcgg gggcggcata tattgtctgg ctggcgtgga aaatcgctac
    79441 cagtccaaca aaggaagatg ggcttcagac aaaaccaatc agtttttggg ccagctttgc
    79501 tttgcagttt gtgaacgtca aaatcatttt gtatggcatt acagcactgt cgacgtttgt
    79561 cctgccgcaa acgcaggcgc taagctggat aattggcgtc agcgttttgc tggcggtgat
    79621 tgggacgttt ggcaatgtgt gctgggcgct ggcggggcac ctgtttcagc gattgtttcg
    79681 ccagtatggt cgccagttaa atatcgtgct cgcgctgctg ctggtctatt gcgcggtacg
    79741 cattttctat taatgaaaaa agcggaagag gtcgccctct tccgcttagt aacttgctac
    79801 ttaagcctta caggctttca gtaaaggtac gagcgataac gtcgcgctgc tgttccggag
    79861 tcagagagtt aaagcgaact gcataaccgg atacacggat ggtcagctgc ggatattttt
    79921 ccggatgctt aactgcatct tccagagttt cacgacgcag aacgtttacg ttcaggtgtt
    79981 gaccaccttc aacgcgaact tctggtttca cttctactgg aacttcacgg tactcaatgt
    80041 cacccagttt gcttactgca accacttcgt cttctgcaaa acctgctttc gcaacgatgc
    80101 aacgcgcttc gcctttttcg ctgtccagca gccagaaaga gttcagcaga tcgtcgttag
    80161 cggctttagt aatctggata cctgtaatca tgtgatgcct ccccggcaaa attatttgat
    80221 ttgttcagcc tgtcgcggcc aattggtaaa accattgttg cttgagtgta tatatactcc
    80281 tcaaacaccc ttgaatcttt gatttaaatc aataaaaacc acacatcaag tatggttgca
    80341 aatggatttt attgttttac atcaacttat gcaggtgtga aattttacca atttacattt
    80401 ttttgcactc gtttaagtct aaaaaatgag catgattttg ttctgtagaa agaagcagtt
    80461 aagctaggcg gattgaagat tcgcgggaga gcgagatggc aaacgaatta acctggcatg
    80521 atgtgctggc tgaagagaag cagcaaccct attttcttaa taccctacag accgtcgcca
    80581 gcgagcggca gtccggcgtc actatctatc cgccacaaaa agatgtcttt aacgcgttcc
    80641 gctttacaga gttgggtgac gttaaagtgg tgattctcgg ccaggatcct tatcacggac
    80701 cgggacaggc gcatggtctg gcattttccg ttcgccccgg cattgcgaca ccgccgtcac
    80761 tgctgaatat gtataaagag ctggaaaata ctattcccgg tttcacccgc cctaatcatg
    80821 gttatcttga aagctgggcg cgtcagggcg ttctgctact caatactgtg ttgacggttc
    80881 gcgcaggtca ggcacattcc cacgccagcc tcggctggga gaccttcacc gataaagtaa
    80941 tcagcctgat taaccagcac cgcgaaggcg tggtgttttt gttgtggggc tcacatgcgc
    81001 aaaagaaagg ggcgattatt gataagcaac gccatcacat actgaaagcg ccgcatccat
    81061 caccgctttc tgcgcatcgt gggttctttg gctgcaacca ttttgtgctg gcaaatcagt
    81121 ggctggaaca acgtggcgag aaaccgattg actggatgcc agtattaccg gcagaaagtg
    81181 agtaaatttg caggaaaacg ccggatggca gcgttgccac ccggctgatt tatcaggctt
    81241 tattctgacg ccaccattca ccaagcaaaa cgccggttgc aacagagata tttagcccgg
    81301 caacgttgcc agtaccatca atcttcacgc gcaaatcgtt cggatcgcgt gcggcatccg
    81361 gtaacccttc atattcctga cccagcacca gtaccatttt cgctggcaga ctggttttga
    81421 acagcggttt cccctgctcg ctggaagtgg tcactacggt gtaacctgcc tgacggaaat
    81481 catccagcac gttaacaatg ttgtcgccag taatcggctg aacgtgctct gcgccgcctt
    81541 ctgcggtacg gatagccgcc cccgactcca gcagtgccgc atcctgcacc acaacgcctt
    81601 tcacgccgaa gtgcgcgcag ctgcgcatca tgccacccag gttatgcggg ttagattcgt
    81661 tttccagcgc cagtacacaa tcctgcgcgc ctgcctggct tacccactgc tgcacggttg
    81721 taccgttacg ctttttgatc aggaagcaaa cgccgccgtg atgttccgtg cctgacgctt
    81781 ttgtcagttc cgcttcatct accacatggt acgctttgcg gtttgctgcc atccagcgca
    81841 aggcttcttt aaaacgcggc gttacactct ggataaacca ggcgcgaaca atcgcttccg
    81901 gacggctctg gaacagtgcc tgacaggcat tttcgccgta gacgcgggtt tcttccgcac
    81961 gctgacgacg caacacttcc ggatcaataa aacttttacc actgatgcca ccgtgatcgg
    82021 ccttttccgg cgtctcatca cccggcgcgc gggaaactgt gcgccacggc gactcttccc
    82081 acttgcggtc acggggctga ctctgtttgt catcgcgggc ggggcgacgg ccaccgtcag
    82141 cacgagattt tcctggacgc ccgccccctt tcccggtacg cgggttgtgg gtacgtttat
    82201 cagaatcatc atcactgcgg acatacatca ctttgacctt gccgctttta cctttcattt
    82261 catcgttcat gcttttctcc accagcgctg cgcgaagcgc gcagattacc cgaagtccgc
    82321 gcggttcgcc atgatttcgt accaaagcct gcgactatca tacctattga ataaaacaga
    82381 ttgttgtctg gaacaatgtc cccgataata tgtaacatat tagaaacata ccggcgtcgt
    82441 tgccgataag tctccttact catcccgagg ttagttatga ataccgtttg tacccattgt
    82501 caggccatca atcgcattcc cgacgatcgg atcgaagatg cggcaaaatg cggacgctgc
    82561 ggtcacgact tgtttgacgg agaggtgatt aatgcgaccg gtgaaacgct cgacaaattg
    82621 ctgaaagatg atctacctgt ggtgatcgac ttctgggcac cgtggtgcgg cccctgccgt
    82681 aatttcgcac caatttttga agatgtcgcg caagagcgta gcggtaaagt gcgctttgtg
    82741 aaagtgaata ccgaagctga acgtgaattg agcagtcact ttggaattcg tagtataccg
    82801 acgatcatga ttttcaaaaa cggtcaggtt gtcgacatgc ttaatggcgc agtaccgaaa
    82861 gcgccgtttg atagctggct gaacgaatct ctttaatctt accggggcgc atcttgtgcc
    82921 ccgttttctc ctctgcgaca atggcgtttt ttcgacgctc tcttatgacc gaaaacgctg
    82981 ttctccagtt acgcgccgag cgtattgcgc gcgcaacacg tccttttctt gcccgcggta
    83041 atcgcgttcg tcgctgccaa cgctgtcttt tgccagagac attatgtctc tgttcgacaa
    83101 ttaccccagc acaagcaaaa agtcgcttct gtttgctgat gttcgacacc gagccaatga
    83161 agcccagtaa taccgggcgt ctgattgctg atattttgcc tgataccgtt gcgtttcaat
    83221 ggtcgcgtac cgaaccctcg ctagatttgc tggatctggt acaaaacccg gactatcagc
    83281 caatggtggt ctttcccgcc tcgtatgctg atgagcaacg ggaagtgatc ttcacaccac
    83341 ctgccggtaa gcctccgcta tttatcatgc tcgatggtac ctggcctgaa gctcgcaaga
    83401 tgtttcgtaa aagtccgtat ctggataatc ttcccgtcat ttccgtcgat ctttcccggc
    83461 tttctgccta tcgcctgcgt gaagcccagg ctgaaggcca atattgtact gccgaggtag
    83521 ccatcgcact gttagatatg gccggcgata ccgaagccgc aacgggctta ggcgagcatt
    83581 tcactcgttt taaaacgcgc tatctggcag gaaaaacgca acatctgggt agcatcacag
    83641 cagaacagtt agaaagcgtt taaaatcatt cggtcacttc tgcgggagac cggtatgagt
    83701 cagcgaggac tggaagcact actgcgacca aaatcgattg cggtaattgg cgcatcgatg
    83761 aaatccaatc gagcaggtta cctgatgatg cgtaacctgc tggcgggagg ctttaacgga
    83821 ccggtactcc cggtgacgcc agcctggaaa gcggtgttgg gtgtgttggc ctggccggat
    83881 attgccagct tgccctttac acccgacctt gcggttttat gtaccaatgc cagccgtaat
    83941 cttgctcttc tggaagagct cggcgagaaa ggctgtaaaa cctgcattat tctttccgcc
    84001 ccggcatcgc aacatgaaga tctccgcgcc tgcgccctgc gccataacat gcgcctgcta
    84061 ggaccaaaca gtctggggtt actggctccc tggcaaggtc tgaatgccag cttttcgcct
    84121 gtgccgatta aacgcggcaa gctggcgttt atttcgcaat cggctgccgt ctccaatacc
    84181 atcctcgact gggcgcaaca gcgtgagatg ggcttttcct actttattgc gctcggcgac
    84241 agcctggata tcgacgttga tgaattgctc gactatctgg cccgcgacag taaaaccagc
    84301 gccatcctgc tctatctcga acagttaagc gacgcacgac gctttgtttc ggcggcccgt
    84361 agtgcctctc gcaacaaacc gattctggtg attaaaagcg gacgtagccc ggcggctcaa
    84421 cgtctgttga acaccacggc aggaatggat ccggcgtggg atgcggctat tcagcgtgcc
    84481 ggtttgttgc gggtacagga tactcacgag ctgttttcgg cggtggaaac cctcagccat
    84541 atgcgcccgc tgcgtggcga ccggctgatg attatcagca acggtgctgc gcctgccgcg
    84601 ctggcgctgg atgccttatg gtcacgcaat ggcaagctgg caacgctaag cgaagaaacc
    84661 tgccagaaac tgcgcgatgc actgccagaa catgtggcaa tctctaaccc gctcgatcta
    84721 cgcgatgacg ccagcagtga gcactatatt aaaacgctgg atattctgct ccacagccag
    84781 gattttgacg cgctgatggt tattcattcg cccagcgccg ctgctcccgc aacagaaagt
    84841 gcgcaagcat taattgaagc ggtaaagcat catccccgca gcaagtatgt ttctctgctg
    84901 acgaactggt gcggcgagca ctcctcgcaa gaggcacgac gattattcag tgaagccggg
    84961 ctgccgacct atcgcacgcc ggaaggaacc atcactgctt ttatgcatat ggtggagtac
    85021 cgacgtaatc agaagcaact acgcgaaacg ccggcgttgc ccagcaatct gacctctaat
    85081 accgcagaag cgcatcttct gttgcaacag gcgattgccg aaggggctac gtcgctcgat
    85141 acccatgaag ttcagcccat cctgcaagcg tatggcatga acacgctccc cacctggatt
    85201 gccagcgata gcaccgaagc ggtgcatatt gccgaacaga ttggttatcc ggtggcgctg
    85261 aaattgcgtt cgccggatat tccacataaa tcggaagttc agggcgtaat gctctacctg
    85321 cgtacagcta atgaagtcca gcaagcggca aacgctattt tcgatcgcgt aaaaatggcc
    85381 tggccgcagg cgcggatcca cggcctgttg gtgcaaagta tggctaaccg tgctggcgct
    85441 caggagttgc gggttgtggt tgagcacgat ccggttttcg ggccgttgat catgctgggc
    85501 gaaggcggtg tggagtggcg tcctgaagat caagccgtcg tcgcactacc gccgctgaac
    85561 atgaacctgg cccgctatct ggttattcag gggatcaaaa gtaaaaagat tcgtgcgcgc
    85621 agtgcgctac gcccattaga tgttgcaggc ttgagccagc ttctggtgca ggtttctaac
    85681 ttgattgtcg attgcccgga aattcagcgt ctggatattc atcctttgct ggcttctggc
    85741 agtgaattta ccgcgctgga tgtcacgctg gatatcgcgc cgtttgaagg cgataacgag
    85801 agtcggctgg cagtgcgccc ttatccgcat cagctggaag aatgggtaga attgaaaaac
    85861 ggtgaacgct gcttgttccg cccgattttg ccagaagatg agccacagct tcagcaattc
    85921 atttcgcgag tcaccaaaga agatctttat taccgctact ttagcgagat caacgaattt
    85981 acccatgaag atttagccaa catgacgcag atcgactacg atcgggaaat ggcttttgtg
    86041 gcggtacgac gtattgatca aacagaagag atcctcggcg tcacgcgtgc gatctccgac
    86101 cctgataaca tcgatgccga atttgccgtg ctggttcgct cggatctcaa agggttaggc
    86161 ttaggtcgac gcttaatgga aaagttgatt acctatacgc gagatcacgg actacaacgt
    86221 ctgaatggta ttacgatgcc aaacaatcgt ggcatggtgg cgctggcccg caagctcggg
    86281 tttaacgttg atatccagct cgaagagggg atcgttgggc ttacgctaaa tcttgcccag
    86341 cgcgaggaat catgagtaag gtactggaaa tgttgaccac ttaatcggga ctggtgttat
    86401 tattgcccgc ttatgtcgtc tgcattgcac agaggaccct tcaatgaaca gagaagaaat
    86461 gcactgtgat gttgtcaaaa tttaagcgta ataaacatca acaacacctt gcccaactac
    86521 ccaagatttc tcaatcagtt gatgatgtcg atttctttta cgctcccgcc gacttccggg
    86581 agacgttgct ggaaaaaata gccagcgcga agcagcgcat ttgcattgtt gccctgtatc
    86641 tcgaacagga tgacggtggc aaaggcattc tgaacgcgtt gtatgaggct aaaaggcagc
    86701 gtccggaact ggatgtgcgg gtgctggtcg actggcatcg tgcacaacgt ggacgcattg
    86761 gcgctgcggc atctaacact aacgctgact ggtactgccg catggcgcag gaaaatccgg
    86821 gcgtagatgt tccggtttat ggcgttccaa tcaatacccg tgaagccctt ggtgttctgc
    86881 actttaaagg ctttatcatc gacgatagcg tactttatag cggtgccagc ctgaacgatg
    86941 tttacctgca tcagcacgat aaatatcgct acgaccgtta tcatctgatc cgtaaccgta
    87001 agatgtcaga cattatgttt gaatgggtta cacagaatat tatgaatggc cgcggcgtta
    87061 atcgtctgga tgatgttaat cggccaaaaa gcccggaaat caagaacgac attcgcctgt
    87121 tccgccagga gctgcgtgat gccgcttatc atttccaggg cgatgccgac aacgatcagc
    87181 tttccgtaac gccgctagtg gggctgggga aatcgagtct gttgaacaag accattttcc
    87241 atcttatgcc ttgtgccgag cagaaactaa ccatctgtac gccatacttc aacctgccag
    87301 caatccttgt gcgcaatatt atccagttgc tgcgcgaagg gaaaaaggtc gaaattattg
    87361 ttggtgataa aaccgcgaac gacttctaca ttccggaaga tgaacctttc aagataattg
    87421 gcgcattgcc ttatctctat gagatcaatc tgcgtcgttt cctgagccgt ttgcagtatt
    87481 acgtcaatac tgaccagcta gtggttcggt tatggaaaga tgacgacaac acctatcacc
    87541 taaaagggat gtgggttgat gataagtgga tgttgatcac cggtaataac ctgaacccgc
    87601 gcgcctggcg tctggatctg gaaaacgcca ttttgatcca cgatccgcaa ctggagcttg
    87661 cgccacagcg agagaaagaa ctggagctga tccgcgagca taccaccatc gttaagcact
    87721 atcgcgatct gcaaagtatt gccgattatc cggtgaaggt tcgtaaactc atccgtcgtt
    87781 tgcgccgtat ccgcatcgac cgattaatta gccgcatcct gtaatcacaa ccccgtcctg
    87841 tacggggttt gttttggagg ccacgttttg cgtattcttt ttgtctgttc gctattgtta
    87901 ctttctggat gcagccatat ggctaacgat agctggagcg ggcaggataa agctcaacac
    87961 tttatcgcct cggcgatgct ttccgccgcc ggaaatgaat atgcacagca tcaggggatg
    88021 agtcgcgatc gcagtgccac tttcgggctg atgttctctg ttgggctggg cgcgtcaaaa
    88081 gaactttggg atagccgccc cgaggggagc ggctggagct ggaaagattt tacctgggat
    88141 gtcgccggtg caactactgg ctataccgtc tggcaactga cccgtcacta aagacgcatc
    88201 cccttccctt tgcgatgtag catcaaagaa accagaaacg ccaccacggc catcaaggtc
    88261 acataccaga agaaggctgt ttccattcct attgatttca gcgacaacgc tacgtactcc
    88321 gccgaaccac caaatatagc attggcgacc gcatatgaca gaccaacacc taatgcacga
    88381 acctgtgccg ggaacatctc agccttcagt attccactga ttgaagtata aaaactcacg
    88441 atcagcaggg cacacatcac cagaccaaaa gcggcataag gcgaggaaac gttttgtaat
    88501 gctgagagaa taggaacggt aaaaatggct gccagcgaac cgaaacataa cattgaggta
    88561 cggcgaccaa tcttatccga cagcgcgcca atgagtggtt gaataagcat gaatacaaac
    88621 aatgcggcag tcataatgcc actcgccacg ttggcatgca ttcccgcagt atttaccaga
    88681 tacttctgca tataagtggt gaaggtatag aaacaaaggg agcccgcagc ggtaaaaccg
    88741 agaaccatga tgaatgcacg gcgattgcgc cataatcctt tcagagatcc agcttctttt
    88801 aaagcgcgcg tttcttgttg cgaagtttca tctaactgac gacgtaacca caacgccaca
    88861 acagctaaca cagctcctaa cgcgaaagga atacgccatc cccactctct gagtgcagcg
    88921 tcttccatgg tatgttgtaa aaccacgaca accagtaggg ctagcagttg tccgccgatc
    88981 aacgtcacat actgaaatga tgcgtaaaaa cctttgcgcc cttcaacggc aacttcactc
    89041 atataggtgg cgctggtgcc atattctccg ccaacggata atccctgaaa taaacgagcg
    89101 agaagcaata atgccggagc ccacgtacct atagtttcat aacctgggag gcaggcgata
    89161 accagcgatc cgaaacacat catacacacc gataacagca tcgatttttt gcgaccatgt
    89221 ttatcggcta tgcggccaaa tagccaaccg cctattgggc gcatcaggaa tcccgcagca
    89281 aaaacacctg ctgtttgtag tagttgagtc gtcgtgttcc cggaagggaa gaaaatgtgg
    89341 gcgaagtaga gtgaacagaa cgagtagaca tagaaatcga accactcgac cagattacct
    89401 gaagaggccc ccacaattgc ccaaatgcgg cgacgagtat cactacttgt cagtttgctg
    89461 tctgccatta cagtactttc agccatgcca ttatgtctcc tgccgtaatc cgatgctttt
    89521 gtcggtcgct tttgtttatt tctattggta aagaaaatgt tatacatttg ttgcatatca
    89581 ttatgcaacc ttaaccacga atttagttag caggaaaatg gttattgagg agcttaagga
    89641 taaatttctg gtaaggagga cacgtatgga ggtgggcaag ttggggaagc cgtattcgct
    89701 gctgaatcta gcttatgtgg gagtataaga cgcgccagcg tcacatccgg catttattcc
    89761 gcaccagcgc aaaaaggcca tccgtcagga t
//
LOCUS       DAPHCV010000002        83607 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.002, whole genome shotgun sequence.
ACCESSION   DAPHCV010000002 DAPHCV010000000
VERSION     DAPHCV010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 83607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.002"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..562)
                     /locus_tag="RH033_000081"
     CDS             complement(<1..562)
                     /locus_tag="RH033_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2989329.1"
                     /translation="MSRRGTQFSNAKVTNPTLRIPFSSSDLGAIVNAGGGAKVLVDVT
                     AEYRQGLVRNLTTSKHYLESKLSEYPGSLGTLVFKLRDQGIAKTHRPNKIAQEAGLQN
                     AGHAKIDEMLVAAHAGCFDVLESVILNRNIKAILANLSAIERIEPWDENRKVPGGTDG
                     LFESSNILVRLFEYTGEDATYNNYENV"
     gene            complement(537..1691)
                     /locus_tag="RH033_000082"
     CDS             complement(537..1691)
                     /locus_tag="RH033_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000385105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDV2989330.1"
                     /translation="MDYLSEVLKIIEGATKANASMASNYAGLLADKLEQKGEVKQARM
                     IRERLLRAPQALAGAQRAGGGISLGSLPVDIDSRLNTVDVSYPKLDSSEIFLPAAIST
                     RVEEFITNVQRYDEFVKADAALPSRMLVYGKPGTGKTMLSKYIATRLDFPLLTVRCDT
                     LISSLLGQTSKNLRQVFDYVMQRPSVLFLDEFDALAGARGNERDIGELQRVVISLLQN
                     MDAASEDTVIIASTNHEQLLDPAIWRRFSFRIPMPLPDIHQRELIWKNRLKNMICSDL
                     DLSDLSRKSEGLSGAIIEQVSLDARRDAVIEGASVINHHKLYRRLYLAQSLMEGVNLS
                     TYEDEIRWLRSKDKKLFSIRVLANLYKLTSRVISNILKESGAYEQKGYTV"
     gene            complement(1885..2316)
                     /locus_tag="RH033_000083"
     CDS             complement(1885..2316)
                     /locus_tag="RH033_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HDV2989331.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRRSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKPSVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEVMQRVLAFYQNGFRERLGDQG"
     gene            complement(2332..3093)
                     /locus_tag="RH033_000084"
     CDS             complement(2332..3093)
                     /locus_tag="RH033_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HDV2989332.1"
                     /translation="METVFDALKAMGKATSIELAARLDISREEVLNELWELKKAGFVD
                     KSAYTWRVADNNVQQEQPAPAELPEETTTATVAKISESDLTATIEQRGPLTADELATL
                     FGTTSRKVASTLAMAISKARLIRVNQNGKFRYCMPGGNLPAEPKAASVAETDGKAFPQ
                     LAGVALPVQEAATQEDIKTETVADIVQSLPSFTETQADNLILPSLHMANRELRRAKNH
                     VQKWERVCAALRELNKHRDIVRQITDSSRRVVSEK"
     gene            complement(3116..3862)
                     /locus_tag="RH033_000085"
     CDS             complement(3116..3862)
                     /locus_tag="RH033_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDV2989333.1"
                     /translation="MKNIATGDVLERIRRLAPSHVTAPFKTVAEWREWQLSEGQKRCE
                     EINRQNRQLRVEKILNRSGIQPLHRKCSFSNYQVQNEGQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCQVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEAMKTLLGERI
                     MDRMTMNGGRWVNFNWESWRPNVVQPGIAK"
     gene            complement(3869..4657)
                     /locus_tag="RH033_000086"
     CDS             complement(3869..4657)
                     /locus_tag="RH033_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2989334.1"
                     /translation="MASNWIKLEVITPDKPEIFRLAEILNIDPDAALGKVIRFWAWAD
                     QQMIDGNAECNARGVTKSAIDRITFMAGFADALIQVGWLVETNGVLSLPNFERHNGKS
                     SKKRAVTNERVTKIRELKRKGNAASVTKTDQKALPEEEKEEDINTYLPLNPPRQKRAS
                     KKFEPEAIELPDWLPETLWHEWVQFRQALRKPIRTEQGANGAIRELEKFRQQGFSPEQ
                     VIRHSIANEYQGLFAPKGVRPETLLRQVNTVSLPDSAIPPGFRG"
     gene            complement(4735..5157)
                     /locus_tag="RH033_000087"
     CDS             complement(4735..5157)
                     /locus_tag="RH033_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2989335.1"
                     /translation="MKIKHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETNAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQDALSTAIDAHDDAVQAVYRKAHFSGGGSPSDSVVVH"
     gene            complement(5154..5408)
                     /locus_tag="RH033_000088"
     CDS             complement(5154..5408)
                     /locus_tag="RH033_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDV2989336.1"
                     /translation="MNQKTLEDVIKTVRVSVVADVCGVSQRAIYKWMDNGKLPRTEYT
                     GETNYAEKIAHASNGLFSADAILTIGRNKTTTKKLMGVDS"
     gene            5488..5907
                     /locus_tag="RH033_000089"
     CDS             5488..5907
                     /locus_tag="RH033_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV2989337.1"
                     /translation="MVHEDKARKEFASRLALACENAGYEQHGRQAEIARRMKLTPKAV
                     SKWFNGETIPRREKLRELATLIGTTPTYLLGEDTEESGQIRFYQELNPRQKIIIDLLD
                     ELPDSETDELLKTLEEKKQKYNAIYEELARKKKQKAS"
     gene            6150..6329
                     /locus_tag="RH033_000090"
     CDS             6150..6329
                     /locus_tag="RH033_000090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2989338.1"
                     /translation="MQQLVPPPGVKGIDKMVHYEVVQYLMDCCGITYNQAVQALRSND
                     WDLWQAEVAIRSNKM"
     gene            6340..6495
                     /locus_tag="RH033_000091"
     CDS             6340..6495
                     /locus_tag="RH033_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaF family protein"
                     /protein_id="HDV2989339.1"
                     /translation="MQKIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTETGARRW
                     LEKHTVS"
     gene            complement(6492..6980)
                     /locus_tag="RH033_000092"
     CDS             complement(6492..6980)
                     /locus_tag="RH033_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection exclusion protein B"
                     /protein_id="HDV2989340.1"
                     /translation="MLDVFTPLLKLFANEPLERLMYTIIIFGLTLWLIPKEFTVAFNA
                     YTEIPWLFQIIVFAFSFVVAISFSRLRAHIQKHYSLLPEQRVLLRLSEKEIAVFKDFL
                     KTGNLIITSPCRNPVMKKLERKGIIQHQSDSANCSYYLVTEKYSHFMKLFWNSRSRRF
                     NR"
     gene            7422..7643
                     /gene="kilR"
                     /locus_tag="RH033_000093"
     CDS             7422..7643
                     /gene="kilR"
                     /locus_tag="RH033_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HDV2989341.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     NLTTKTFITDRMIKVFLGRDGLPVKAESW"
     gene            7643..7813
                     /locus_tag="RH033_000094"
     CDS             7643..7813
                     /locus_tag="RH033_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2989342.1"
                     /translation="MTKKIKCAYHLCKKDVEESKAIERMLHFMHGILSKDEPRKYCSE
                     ACAEKDQMAHEL"
     gene            7888..8163
                     /locus_tag="RH033_000095"
     CDS             7888..8163
                     /locus_tag="RH033_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2989343.1"
                     /translation="MITNYEATVVTTDDIVHEVNLEGKRIGYVIKTENKEAPFTVVDI
                     DGPSGNVKTLDEGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            8265..10865
                     /gene="recE"
                     /locus_tag="RH033_000096"
     CDS             8265..10865
                     /gene="recE"
                     /locus_tag="RH033_000096"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HDV2989344.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASNDFESTCATLDYLIVKSG
                     KKLSSYFKAVATNFPVVNDLPAEGEIDFTWSERYQLSKDSMTWELKPGAAPDNAHYQG
                     NTNVNGEDMTEIEENMLLPISGQELPIRWLAQHGSEKPVTHVSRDGLQALHIARAEEL
                     PAVTALAVSHKTSLLDPLEIRELHKLVRDTDKVFPNPGNSNLGLITAFFEAYLNADYT
                     DRGLLTKEWMKGNRVSHITRTASGANAGGGNLTDRGEGFVHDLTSLARDVATGVLARS
                     MDLDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGLDYSRAIVVASVKEAPIG
                     IEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDDEEKPQP
                     SGTTAVEQGEAETMEPDATEHHQDTQPLDAQSQVNSVDAKYQELRAELHEARKNIPSK
                     NPVDDDKLLAASRGEFVDGISDPNDPKWVKGIQTRDCVYQNQPETEKTSPDMNQPEPV
                     VQQEPEIACNACGQTGGDNCPDCGAVMGDATYQETFDEESQVEAKENDPEEMEGAEHP
                     HNENAGSDPHRDCSDETGEVADPVIVEDIEPGIYYGISNENYHAGPGISKSQLDDIAD
                     TPALYLWRKNAPVDTTKTKTLDLGTAFHCRVLEPEEFSNRFIVAPEFNRRTNAGKEEE
                     KAFLMECASTGKTVITAEEGRKIELMYQSVMALPLGQWLVESAGHAESSIYWEDPETG
                     ILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAYYDYRYHVQDAFYSDGYEAQFGVQPT
                     FVFLVASTTIECGRYPVEIFMMGEEAKLAGQQEYHRNLRTLSDCLNTDEWPAIKTLSL
                     PRWAKEYAND"
     gene            10858..11667
                     /gene="recT"
                     /locus_tag="RH033_000097"
     CDS             10858..11667
                     /gene="recT"
                     /locus_tag="RH033_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HDV2989345.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAVKNSDVISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFSFEFGLDEKLIH
                     RPGENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSLSKAGNNGPWVTHWEEMAK
                     KTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
     gene            11724..11918
                     /gene="ralR"
                     /locus_tag="RH033_000098"
     CDS             11724..11918
                     /gene="ralR"
                     /locus_tag="RH033_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HDV2989346.1"
                     /translation="MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEK
                     EALERWNKRTTGNNNGGVHV"
     gene            11911..12120
                     /gene="rcbA"
                     /locus_tag="RH033_000099"
     CDS             11911..12120
                     /gene="rcbA"
                     /locus_tag="RH033_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break reduction protein RcbA"
                     /protein_id="HDV2989347.1"
                     /translation="MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVS
                     REQKVKLINFNCEKLQSSRIALYSN"
     gene            12199..12414
                     /gene="xisR"
                     /locus_tag="RH033_000100"
     CDS             12199..12414
                     /gene="xisR"
                     /locus_tag="RH033_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HDV2989348.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            12416..13651
                     /locus_tag="RH033_000101"
     CDS             12416..13651
                     /locus_tag="RH033_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDV2989349.1"
                     /translation="MSKLPTGVEIRGRYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHEINSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNLWKFAVYSGLRHGELAALAWEDVDLEKGIVNVRRNLTILDMFGPPKTNAGIRTVTL
                     LQPALEALKEQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNGKQKPYYSVS
                     SLGARWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYG
                     MWIDDMNDEQIAMLNARLS"
     gene            13703..14638
                     /gene="ttcA"
                     /locus_tag="RH033_000102"
     CDS             13703..14638
                     /gene="ttcA"
                     /locus_tag="RH033_000102"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDV2989350.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(14767..16140)
                     /gene="dbpA"
                     /locus_tag="RH033_000103"
     CDS             complement(14767..16140)
                     /gene="dbpA"
                     /locus_tag="RH033_000103"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HDV2989351.1"
                     /translation="MSAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPTILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRMLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQSVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPVNSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(16618..17601)
                     /gene="zntB"
                     /locus_tag="RH033_000104"
     CDS             complement(16618..17601)
                     /gene="zntB"
                     /locus_tag="RH033_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HDV2989352.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            17856..19088
                     /gene="dgcM"
                     /locus_tag="RH033_000105"
     CDS             17856..19088
                     /gene="dgcM"
                     /locus_tag="RH033_000105"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HDV2989353.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRNEEETALSCRLVLRKLTEAEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(19109..19672)
                     /gene="smrA"
                     /locus_tag="RH033_000106"
     CDS             complement(19109..19672)
                     /gene="smrA"
                     /locus_tag="RH033_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HDV2989354.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(19867..21381)
                     /locus_tag="RH033_000107"
     CDS             complement(19867..21381)
                     /locus_tag="RH033_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HDV2989355.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTTAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWITDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQVRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SGSDQTSEILASTKLLSQLMLREAQTMAFSDTFLLISGLLFIAFLLVPAMNKSS"
     gene            complement(21378..22406)
                     /locus_tag="RH033_000108"
     CDS             complement(21378..22406)
                     /locus_tag="RH033_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDV2989356.1"
                     /translation="MIISKKQLIGVVAIGIILAGVVFFIWWVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSRKANLES
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLVQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLVRAPID
                     GIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQIAGMKPGMKAEIRADILKG
                     EVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVRIAFDDAKELKQLLRPGLS
                     VTVSVDER"
     gene            22503..23318
                     /locus_tag="RH033_000109"
     CDS             22503..23318
                     /locus_tag="RH033_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV2989357.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGKVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHDYAITRQMI"
     gene            23708..23851
                     /locus_tag="RH033_000110"
                     /pseudo
     CDS             23708..23851
                     /locus_tag="RH033_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            23862..24377
                     /gene="ogt"
                     /locus_tag="RH033_000111"
     CDS             23862..24377
                     /gene="ogt"
                     /locus_tag="RH033_000111"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDV2989358.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISAANPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            24572..25324
                     /gene="fnr"
                     /locus_tag="RH033_000112"
     CDS             24572..25324
                     /gene="fnr"
                     /locus_tag="RH033_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HDV2989359.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            25476..26426
                     /gene="uspE"
                     /locus_tag="RH033_000113"
     CDS             25476..26426
                     /gene="uspE"
                     /locus_tag="RH033_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HDV2989360.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(26476..26733)
                     /locus_tag="RH033_000114"
     CDS             complement(26476..26733)
                     /locus_tag="RH033_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HDV2989361.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            26977..28008
                     /gene="ynaI"
                     /locus_tag="RH033_000115"
     CDS             26977..28008
                     /gene="ynaI"
                     /locus_tag="RH033_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HDV2989362.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAAILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPDQGR"
     gene            complement(28059..29672)
                     /gene="mppA"
                     /locus_tag="RH033_000116"
     CDS             complement(28059..29672)
                     /gene="mppA"
                     /locus_tag="RH033_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HDV2989363.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKAAPDQLGVTA
                     VDAQTLKIQLDKPLPWFVNLTANFAFFPVQKANVESSKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGLDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(30009..30908)
                     /gene="pgrR"
                     /locus_tag="RH033_000117"
     CDS             complement(30009..30908)
                     /gene="pgrR"
                     /locus_tag="RH033_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HDV2989364.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSAPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            31046..31966
                     /locus_tag="RH033_000118"
     CDS             31046..31966
                     /locus_tag="RH033_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV2989365.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNSRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVDAGSNARTSDISSREYATMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPIQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKVYVAEAISVLAPFFEETL"
     gene            32037..32675
                     /locus_tag="RH033_000119"
     CDS             32037..32675
                     /locus_tag="RH033_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV2989366.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKAGDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            32966..33694
                     /gene="mpaA"
                     /locus_tag="RH033_000120"
     CDS             32966..33694
                     /gene="mpaA"
                     /locus_tag="RH033_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HDV2989367.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASHESGLI
                     LAGTHGDENSSIVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(33669..34634)
                     /gene="ycjG"
                     /locus_tag="RH033_000121"
     CDS             complement(33669..34634)
                     /gene="ycjG"
                     /locus_tag="RH033_000121"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HDV2989368.1"
                     /translation="MRTVKVFEEGWPLHTPFVIAWGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVTTAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            34753..35259
                     /gene="tpx"
                     /locus_tag="RH033_000122"
     CDS             34753..35259
                     /gene="tpx"
                     /locus_tag="RH033_000122"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HDV2989369.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(35303..36844)
                     /gene="tyrR"
                     /locus_tag="RH033_000123"
     CDS             complement(35303..36844)
                     /gene="tyrR"
                     /locus_tag="RH033_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HDV2989370.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRTLTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(36992..38053)
                     /locus_tag="RH033_000124"
     CDS             complement(36992..38053)
                     /locus_tag="RH033_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HDV2989371.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIEDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(38050..39447)
                     /locus_tag="RH033_000125"
     CDS             complement(38050..39447)
                     /locus_tag="RH033_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HDV2989372.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRYKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            39602..40600
                     /locus_tag="RH033_000126"
     CDS             39602..40600
                     /locus_tag="RH033_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV2989373.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNLAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFQLIAGKPSPQNITVATHMSLK"
     gene            complement(40651..41556)
                     /gene="ompG"
                     /locus_tag="RH033_000127"
     CDS             complement(40651..41556)
                     /gene="ompG"
                     /locus_tag="RH033_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HDV2989374.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRISLAYYQEGPVDYSAGKLGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(41601..42683)
                     /locus_tag="RH033_000128"
     CDS             complement(41601..42683)
                     /locus_tag="RH033_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV2989375.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFSLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAREVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLH
                     KPIVMGIRPEDIQPQAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(42697..43356)
                     /gene="pgmB"
                     /locus_tag="RH033_000129"
     CDS             complement(42697..43356)
                     /gene="pgmB"
                     /locus_tag="RH033_000129"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDV2989376.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFKAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     ADLRAQQIPIGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWPRLSAFWQN
                     V"
     gene            complement(43353..45620)
                     /gene="ycjT"
                     /locus_tag="RH033_000130"
     CDS             complement(43353..45620)
                     /gene="ycjT"
                     /locus_tag="RH033_000130"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HDV2989377.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RNVVWCTSNGARYTIVSRRFVSADQLPLIALEITITPLDADALVLISTGIDATQTNHG
                     RQHLDETQLRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETLTLQKRVWIDWRDDRLVALDEWGSASLRQLEMYVQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVVQYWQTTGDESFIAHEGMALLLETAK
                     FWISRTVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALNIARQFGCSDDA
                     FIHRAEIFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQYSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LAQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRNGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(45617..46672)
                     /locus_tag="RH033_000131"
     CDS             complement(45617..46672)
                     /locus_tag="RH033_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDV2989378.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKQGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVTAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHLYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(46682..47470)
                     /locus_tag="RH033_000132"
     CDS             complement(46682..47470)
                     /locus_tag="RH033_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDV2989379.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEKVAACTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(47489..48541)
                     /locus_tag="RH033_000133"
     CDS             complement(47489..48541)
                     /locus_tag="RH033_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HDV2989380.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCEIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(48572..49414)
                     /locus_tag="RH033_000134"
     CDS             complement(48572..49414)
                     /locus_tag="RH033_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDV2989381.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(49401..50282)
                     /locus_tag="RH033_000135"
     CDS             complement(49401..50282)
                     /locus_tag="RH033_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDV2989382.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTTLVVAGSAVLGLSVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(50303..51595)
                     /locus_tag="RH033_000136"
     CDS             complement(50303..51595)
                     /locus_tag="RH033_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV2989383.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKAEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLISELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQTSQKKLDELVEQR"
     gene            complement(51609..53288)
                     /gene="ycjM"
                     /locus_tag="RH033_000137"
     CDS             complement(51609..53288)
                     /gene="ycjM"
                     /locus_tag="RH033_000137"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HDV2989384.1"
                     /translation="MEQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYPQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQQPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLWSIIDDVAPGTVIITETNVPH
                     KDNIAYFGEGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDVERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     HSEEITQELNDEATLRHAVYHQLSRLITLRRSHNEFHPDNDFTIDTVNSSVMCIQRSN
                     ADGNCLTGLFNVSENIQHINITDLHGRDLISEVDILGNEITLRPWQVMWIK"
     gene            complement(53502..53816)
                     /gene="pspE"
                     /locus_tag="RH033_000138"
     CDS             complement(53502..53816)
                     /gene="pspE"
                     /locus_tag="RH033_000138"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HDV2989385.1"
                     /translation="MFKKGLLALTLVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(53891..54112)
                     /gene="pspD"
                     /locus_tag="RH033_000139"
     CDS             complement(53891..54112)
                     /gene="pspD"
                     /locus_tag="RH033_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HDV2989386.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(54121..54480)
                     /gene="pspC"
                     /locus_tag="RH033_000140"
     CDS             complement(54121..54480)
                     /gene="pspC"
                     /locus_tag="RH033_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HDV2989387.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(54480..54704)
                     /gene="pspB"
                     /locus_tag="RH033_000141"
     CDS             complement(54480..54704)
                     /gene="pspB"
                     /locus_tag="RH033_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HDV2989388.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(54758..55426)
                     /gene="pspA"
                     /locus_tag="RH033_000142"
     CDS             complement(54758..55426)
                     /gene="pspA"
                     /locus_tag="RH033_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HDV2989389.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKDREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            55593..56570
                     /gene="pspF"
                     /locus_tag="RH033_000143"
     CDS             55593..56570
                     /gene="pspF"
                     /locus_tag="RH033_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HDV2989390.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSEITSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(56690..57955)
                     /gene="puuE"
                     /locus_tag="RH033_000144"
     CDS             complement(56690..57955)
                     /gene="puuE"
                     /locus_tag="RH033_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HDV2989391.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENAMLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHDVSTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLTNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALND"
     gene            complement(57993..59273)
                     /gene="puuB"
                     /locus_tag="RH033_000145"
     CDS             complement(57993..59273)
                     /gene="puuB"
                     /locus_tag="RH033_000145"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HDV2989392.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVHELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(59275..60762)
                     /gene="puuC"
                     /locus_tag="RH033_000146"
     CDS             complement(59275..60762)
                     /gene="puuC"
                     /locus_tag="RH033_000146"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HDV2989393.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYTAAAENETFETVDPVT
                     QAPLANIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEANAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVEVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(60912..61469)
                     /gene="puuR"
                     /locus_tag="RH033_000147"
     CDS             complement(60912..61469)
                     /gene="puuR"
                     /locus_tag="RH033_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HDV2989394.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(61496..62260)
                     /gene="puuD"
                     /locus_tag="RH033_000148"
     CDS             complement(61496..62260)
                     /gene="puuD"
                     /locus_tag="RH033_000148"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HDV2989395.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            62472..63890
                     /gene="puuA"
                     /locus_tag="RH033_000149"
     CDS             62472..63890
                     /gene="puuA"
                     /locus_tag="RH033_000149"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HDV2989396.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            64039..64203
                     /locus_tag="RH033_000150"
     CDS             64039..64203
                     /locus_tag="RH033_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2989397.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            64193..65578
                     /gene="puuP"
                     /locus_tag="RH033_000151"
     CDS             64193..65578
                     /gene="puuP"
                     /locus_tag="RH033_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HDV2989398.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YDGDRTPVSET"
     gene            65712..65957
                     /locus_tag="RH033_000152"
     CDS             65712..65957
                     /locus_tag="RH033_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HDV2989399.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLALYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            66270..67913
                     /gene="sapA"
                     /locus_tag="RH033_000153"
     CDS             66270..67913
                     /gene="sapA"
                     /locus_tag="RH033_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HDV2989400.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQYIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            67910..68875
                     /gene="sapB"
                     /locus_tag="RH033_000154"
     CDS             67910..68875
                     /gene="sapB"
                     /locus_tag="RH033_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HDV2989401.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMVMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            68862..69752
                     /gene="sapC"
                     /locus_tag="RH033_000155"
     CDS             68862..69752
                     /gene="sapC"
                     /locus_tag="RH033_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HDV2989402.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLILGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            69752..70744
                     /gene="sapD"
                     /locus_tag="RH033_000156"
     CDS             69752..70744
                     /gene="sapD"
                     /locus_tag="RH033_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HDV2989403.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            70746..71552
                     /gene="sapF"
                     /locus_tag="RH033_000157"
     CDS             70746..71552
                     /gene="sapF"
                     /locus_tag="RH033_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HDV2989404.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(71600..72763)
                     /locus_tag="RH033_000158"
     CDS             complement(71600..72763)
                     /locus_tag="RH033_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HDV2989405.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCLLFINETLPENKRGSSLLLTGRSVVQNRRFIRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWIQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGIGGETLLKMSLAITVCYTLALLVALTRIDNQK"
     gene            complement(72772..74145)
                     /locus_tag="RH033_000159"
     CDS             complement(72772..74145)
                     /locus_tag="RH033_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDV2989406.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTGTTAEADGTVSSYTLDLFGRNQSLSRAARETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMASAENSLKIIQRQQQVGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLAENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFAPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQSAFKDVANALARRNTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQAGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            complement(74149..77256)
                     /locus_tag="RH033_000160"
     CDS             complement(74149..77256)
                     /locus_tag="RH033_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HDV2989407.1"
                     /translation="MFSRFFVRRPVFAWVIAILIMLAGILAIRTLPVAQYPDVAPPTI
                     KISATYTGASAETLENSVTQVIEQQLTGLDNLLYFSSTSSSDGSVSINVTFEQGTDPD
                     TAQVQVQNKIQQAESRLPSEVQQTGVTVEKSQSNFLLIAAVYDTTDKASSSDIADWLV
                     SNVQDPLARVEGVGSLQVFGAEYAMRIWLDPAKLASYSLMPSDVQSAIEAQNVQVTAG
                     KIGALPSPNTQQLTATVRAQSRLQTVDQFKNIIVKSQSDGAVVRIKDVARVEMGSEDY
                     TAIGKLNGHPSAGVAVMLSPGANALNTATLVKDKIAEFQRNMPQGYDIAYPKDSTEFI
                     KISVEDVIQTLFEAIVLVVCVMYLFLQNLRATLIPALAVPVVLLGTFGVLALFGYSIN
                     TLTLFAMVLAIGLLVDDAIVVVENVERIMRDEGLPAREATEKSMGEISGALVAIALVL
                     SAVFLPMAFFGGSTGVIYRQFSITIISAMLLSVVVALTLTPALCGSVLQHVPPHKKGF
                     FGAFNRFYRRTEDKYQRGVIYVLRRAARTMGLYVVLGGGMALMMWKLPGSFLPTEDQG
                     EIMVQYTLPAGATAARTAEVNRQIVDWFLINEKANTDVIFTVDGFSFSGSGQNTGMAF
                     VSLKNWSQRKGAENTAQAIALRATKELGTIRDATVFAMTPPAVDGLGQSNGFTFELLA
                     NGGTDRETLLQMRNQLIEKANQSPELHSVRANDLPQMPQLQVDIDSNKAVSLGLSLND
                     VTDTLSSAWGGTYVNDFIDRGRVKKVYIQGDSEFRSAPSDLGKWFVRGSDNAMTPFSA
                     FATTRWLYGPERLVRYNGSAAYEIQGENATGFSSGDAMTKMEELANSLPAGTTWAWSG
                     LSLQEKLASGQALSLYAVSILVVFLCLAALYESWSVPFSVILVIPLGLLGAALAAWMR
                     DLNNDVYFQVALLTTIGLSSKNAILIVEFAEAAVAEGYSLSRAALRAAQTRLRPIIMT
                     SLAFIAGVMPLAIATGAGANSRIAIGTGIIGGTLTATLLAIFFVPLFFVLVKRLFAGK
                     PRRQE"
     gene            complement(77256..78377)
                     /locus_tag="RH033_000161"
     CDS             complement(77256..78377)
                     /locus_tag="RH033_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDV2989408.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTLKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            complement(78526..79092)
                     /locus_tag="RH033_000162"
     CDS             complement(78526..79092)
                     /locus_tag="RH033_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDV2989409.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSDEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            79570..80358
                     /gene="fabI"
                     /locus_tag="RH033_000163"
     CDS             79570..80358
                     /gene="fabI"
                     /locus_tag="RH033_000163"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDV2989410.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDTSIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            80502..81629
                     /locus_tag="RH033_000164"
     CDS             80502..81629
                     /locus_tag="RH033_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HDV2989411.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDPLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVCDC
                     VIINQVIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESLRWRSSYEV
                     EKLPEEHTKSSTAELCQLANTLSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFAACMNSSNEYKKISPLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            81697..>83607
                     /gene="rnb"
                     /locus_tag="RH033_000165"
     CDS             81697..>83607
                     /gene="rnb"
                     /locus_tag="RH033_000165"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HDV2989412.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRVEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTELDFVTIDSASTEDMDDALFAKALP
                     DGKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSTDGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETAYKVTDVI
                     DVTIAEVRMETRS"
BASE COUNT        21166 a        21614 c        20032 g        20795 t
ORIGIN      
        1 taacgttttc atagttgttg taagttgcat cttcacctgt gtactcaaat agtcgtacaa
       61 ggatgtttga tgattcaaac aaaccatcag tgcctcccgg aaccttccta ttctcatccc
      121 aaggttcaat gcgctcaatc gcgcttagat tagccaaaat cgctttaata ttccgattta
      181 aaatgactga ctctaatacg tcaaaacagc cggcatgagc agcaaccaac atttcatcta
      241 ttttggcatg accggcattt tgcaatccag cctcttgagc aattttgttc ggcctatgcg
      301 ttttggctat tccctggtct cttaatttga aaaccaaagt acccaagctt ccagggtact
      361 ctgaaagttt ggattctaaa taatgtttac tggttgttaa atttcttact agcccttgtc
      421 tatattcggc tgttacatca accaatacct ttgccccacc gccagcgttt actattgcac
      481 ccaagtcact actggaaaaa gggattctta acgttgggtt tgtaactttt gcgttactaa
      541 actgtgtacc ccttctgctc atatgctcct gactccttca gaatgtttga aattactctt
      601 gatgtaagtt tgtacaaatt agcaagaact ctgatagaaa ataatttttt atctttagaa
      661 cgtaaccaac gaatttcatc ttcgtaagtg cttaaattta caccttccat aagcgattga
      721 gcaagataca aacgcctata caatttgtgg tgatttatca cacttgcacc ttcaataact
      781 gcatccctac gtgcatccaa gctcacctgt tcaattattg ctccggataa tccttccgat
      841 tttcttgata aatcacttaa atctagatcg ctacatatca tattctttaa acgatttttc
      901 caaattaact ctctctgatg tatgtcaggc agaggcattg gaattctgaa gctaaatcgc
      961 ctccagattg caggatccag aagttgctca tggttagttg aggcaataat taccgtatcc
     1021 tctgatgccg catccatatt ctgcaatagt gaaatgacaa ctcgctgaag ctcacctata
     1081 tctctctcat tacctcttgc tccagctaaa gcatcaaatt cgtctaaaaa aagcactgat
     1141 ggcctctgca ttacataatc gaaaacctgt ctaagatttt tgctggtttg tcccaataaa
     1201 ctactaatca aagtatcgca acgcactgta agaagtggaa aatctaagcg ggtagcgatg
     1261 tacttagata acatagtctt acctgttcct ggctttccat acacgagcat acgactcggc
     1321 aatgctgcat cagctttaac aaactcatca taacgttgaa cattagtgat aaactcttca
     1381 acacgggtac tgattgctgc aggcagaaaa atctctgaac tgtctaattt aggataactg
     1441 acatcaacag tgttgagtcg actatcaata tctaccggta atgagcccag agatatccca
     1501 cctccagccc tttgagctcc tgccaacgcc tggggagctc taagcaacct ttctcttatc
     1561 attctggctt gcttgacctc ccctttttgt tcgagcttat ctgccagcaa cccagcataa
     1621 ttactagcca tcgaagcatt tgcctttgtt gcaccttcaa tgatttttaa cacttctgat
     1681 aaataatcca ttcaacactc ccacaacaaa aagccacagc aatcaaaaaa tgagcacgca
     1741 gaatcaaaat actctctaat cgtaacgcat tttgagattt ttgatacgtt tttttgggat
     1801 tatgtaacgc caaatggtca ttttgtgtca gaaaaataaa tcttagtacc gagaatatgt
     1861 agcttccatc cttcatcgtt ctattcatcc ctgatctcca agcctctctc gaaaaccatt
     1921 ctgataaaac gccagcacac gctgcataac ttcgctcttc cggcactcgc gacagattat
     1981 gttcagacgc ctgtcgtagc ggcgtatttc tccgtctggt aatgaccaga taaggtccgg
     2041 atcaaccaca gatggtttct tcacctttgc cctagatagt tttttgcggg cattttgcca
     2101 gtccttacga gcctgttcag acgggaataa cccgtaacca gagttgtata catcgccact
     2161 ggcaaccagc tctctggcga gaacgctcat cagatatctt gtcgcacctg tcttggcttc
     2221 cagttgccgt aacgtctcgc gcccactccg gcgtactagt tcaacaacct gccctttaat
     2281 tttttcccgc tcttcttgtg taaatacttt tgccataagc gcctccggca atcacttttc
     2341 cgatacaaca cggcgggaag aatcagtaat ctgtcgaaca atatcccggt gcttgttcag
     2401 ctcccgcagc gcggcgcaga ctcgctccca cttctggaca tgatttttcg cccgacgcag
     2461 ttcacggttt gccatatgca gcgatggtaa aatcaggtta tccgcttgcg tttcagtaaa
     2521 cgatggcaac gactgcacaa tgtccgccac agtttctgtt ttaatatctt cctgtgttgc
     2581 agcttcctgt actggtaacg caacaccggc tagctgagga aaggctttac catctgtttc
     2641 cgctaccgat gcagctttcg gctctgctgg taaattaccg cccggcatgc agtaacgaaa
     2701 tttaccgttc tgattaacgc gaatcagacg agctttgctg attgccattg ccagcgttga
     2761 agccactttg cgggatgtgg taccgaacaa cgtagccagt tcatccgcag ttagtggacc
     2821 acgttgttca atcgtcgcag ttaaatcgct ctctgaaatt ttcgcgactg ttgccgtggt
     2881 agtttcttcc ggcagttctg ccggcgctgg ctgttcctgc tgaacgttgt tatcagccac
     2941 acgccaggtg tacgcgcttt tatcaacgaa accagccttt ttcagttccc atagttcgtt
     3001 cagcacttct tcacggctga tatcaagtcg cgcagcaagt tctatggatg tggcttttcc
     3061 cattgctttc agtgcgtcaa aaacagtctc cattaaattt ttctcccggt aaaaattact
     3121 tcgcaattcc tggctggacg acattcggac gccagctctc ccagttaaaa ttcacccatc
     3181 gcccgccgtt catggtcatg cgatccataa tcctctcgcc gagcaatgtt ttcatggcct
     3241 cataattcag gtttgtcagc atccccacgc tgcgcatcga cgctgtccgg cgatcaacaa
     3301 tctggtgcag caccacctgc tcgttttttg tctcgcgctg aatgccaatt tcatcaagaa
     3361 ccagcagatc cacttggcac agttcccgca aaaatttttc gcctgattgc ccgtcgtcat
     3421 agctggcgtg tagggcactc atgacatcag ccacggtaac cacaatcact gtctggccat
     3481 ctttcagcag gcgattcccg atagctgccg ctaagtggtt cttcccggta ccaggttttc
     3541 cgctgaacgc aaaatttgta cacccggtca tcagttcatc agcgatggat ttcgcctgac
     3601 tcaacgcgta tcgctgccct tcgttctgca cctggtaatt cgaaaacgag catttgcggt
     3661 gcaatggctg gatgccagag cgattcagaa ttttttccac ccgcaactga cgattctgac
     3721 ggttgatctc ctcacaacgt ttctggcctt cggaaagttg ccactcgcgc cactccgcta
     3781 ccgtcttgaa tggcgcggtt acatgtgacg gggccagtct gcggatacgt tcaagaacgt
     3841 cgcctgtcgc aatatttttc atggtcagtt accccctgaa gcctggcggg atcgcactat
     3901 ccggtaacga gacggtgtta acctgtcgga gtaacgtctc aggtcgaaca cctttcggcg
     3961 cgaacaagcc ctggtattcg ttggcgatgc tgtgtcgaat cacctgctca ggtgaaaaac
     4021 cctgctggcg gaatttttcc agctcccgta tcgccccgtt agcgccctgc tccgttcgaa
     4081 tcggttttcg caatgcctgc ctgaactgaa cccactcatg ccagagtgtt tccggcaacc
     4141 agtcaggcag ctcgatagcc tccggttcga attttttaga cgctcgtttt tggcgagggg
     4201 gatttagggg gagataagta tttatatctt cctctttctc ttcctctggt aacgcttttt
     4261 gatccgtttt tgtaacgctg gcagcgttac cttttcgttt cagttctcgt atttttgtta
     4321 ctctctcgtt tgtaaccgcc cgttttttag agcttttccc gttatggcgc tcaaagttag
     4381 gaagcgacaa cacaccatta gtttcgacca gccatccaac ctgaattaac gcatcagcaa
     4441 aaccagccat aaaagtgatg cgatctattg cactttttgt aacgccgcga gcgttacact
     4501 ctgcgttacc gtctatcatt tgttgatccg cccatgccca gaagcgaatg actttcccta
     4561 atgcggcatc tggatcaata ttcagaatct cagcaagcct gaatatttcc ggcttatccg
     4621 gcgtaataac ttcgagcttt atccagtttg aagccatttg ttttcacctt gtaacgctcg
     4681 cagcgttaca tttaactgat accgaacaaa acagttcggt acgattaatt tcaatcaatg
     4741 cactacgaca gaatcgctag gagaaccgcc gccgctgaaa tgtgctttac ggtaaacggc
     4801 ctggactgca tcatcatgcg catcaattgc cgtactcagt gcatcctgtg ccgccagtaa
     4861 tgcacggcgt tccagggtat cgaagatgca gagtcggtga cgcagctcgc gaggaagaat
     4921 tgccagaacc gcagggatca gtttctgaat tttttccctt tgcgcattcg tttcaccttt
     4981 caaccaacga tgatagatat tctgctgatt gttccagtcc ttgcctggta ccaggggcaa
     5041 ttcgccgccc ccctggcgca gatattcttc agtaattgcg ttagcgaccc acgcctgccc
     5101 tttttcggct gccagggcta acaacactga ttcgatgtgc tcatgcttga ttttcatgaa
     5161 tcaactccca tcagcttttt cgtagtagtt ttatttctgc caatagttaa aattgcatcg
     5221 gcagaaaata atccgtttga tgcatgagcg attttttcag cgtaatttgt ttcgccggta
     5281 tattctgtgc gaggcaattt tccgttatcc atccatttgt agattgctct ttggctgaca
     5341 ccacaaacgt cggccacaac agaaacgcga acagttttga ttacatcttc aagtgttttc
     5401 tggttcatat caccctcaca atgtgaactt tgagtacatg ctataacaga actgacagta
     5461 cattcaagag cgaatatcat tgaacttatg gttcatgaag ataaagcgcg taaagagttc
     5521 gccagtaggc ttgcgctagc ctgtgaaaac gctggttatg aacaacatgg aaggcaggca
     5581 gaaattgccc gtcgaatgaa attaacacca aaagcggtta gcaaatggtt taatggtgaa
     5641 acaattcctc gccgagagaa attaagggaa ttagcaacac tcattggaac aacaccaacc
     5701 tatcttttgg gagaggatac agaagaaagt ggacagatac gtttctatca ggagttaaat
     5761 ccaagacaaa aaatcatcat tgaccttctg gacgagctcc ctgacagtga gacagatgaa
     5821 cttttaaaaa ctcttgagga gaaaaaacag aagtacaatg caatttacga agagttagca
     5881 cgaaagaaaa aacaaaaagc ctcttaaacc agcataaatc cggtagcgtc cccctccggg
     5941 tttgtgcttc actttatccc gtctcatttt tttatacata aaatgtactt aaagtacttt
     6001 acaatgatga acacaaagta cattatatac ctaccaaccc accccgcccc acagaacgcc
     6061 gggcaatact tcgagttacc aggcagtggt caggggttaa gtagccagcc cgaggcgtat
     6121 gaacatgacg gcgggattca aattttgcag tgcagcagtt agttccgcca cccggcgtta
     6181 aggggataga taagatggtg cattacgaag tagttcagta tttgatggat tgttgcggta
     6241 tcacttacaa ccaggctgtg caggctttac gcagcaacga ctgggatctc tggcaggcag
     6301 aagtcgctat acgtagcaac aagatgtgag attcgcaaaa tgcaaaaaat cgacctcggc
     6361 aacaacgaat ccctggtgtg cggcgtgttc cccaaccagg atggaacgtt cactgccatg
     6421 acgtatacca aaagcaaaac atttaaaacc gaaactggtg cgcgccgatg gttggagaag
     6481 cacacagtaa gctaacgatt aaaacgtcta ctcctgctgt tccagaataa cttcataaaa
     6541 tgggagtatt tttcggtgac gagataataa gaacagtttg cgctatcact ctgatgttga
     6601 atgatgccct tccgttctaa ttttttcata accgggttac ggcaaggaga agtgataata
     6661 agatttcctg ttttaaggaa atctttaaat acagcgattt ctttctcaga taaacgaagc
     6721 aatactcgtt gctctggtag taatgaataa tgcttttgaa tatgtgctcg caatcttgag
     6781 aaggaaatgg cgaccacgaa agaaaaggca aaaacgataa tctgaaagag ccaaggtatt
     6841 tcagtataag cattgaatgc gacagtaaac tctttcggta tcagccagag agtgagacca
     6901 aaaatgataa tcgtatacat aagtctttcg agtggctcgt tagcaaaaag tttcaacaat
     6961 ggagtaaata catccaacat atcaataact ctcaactgta agggtattga aatgttaaca
     7021 caagctctcg ctgtaggggt atagccgaga ccaccgaagc ccggaggtgg tgaaataaaa
     7081 ccgggcacaa cacgaaggcg catttccgat atccataaag agtcggtctt gtctgttaaa
     7141 tttaaatggt gggagtgcgc ctccggttgt aaataacgac attgctgtgt gtagtcctgg
     7201 cggcatcagt ttttttcttg aagttcggct gatgtccgcc ctttttaaag tgaattttgt
     7261 gatgcggtga atgcggctaa gcgcacgtgg cacagttaaa agtcatgtta gtccttattg
     7321 gtttgggtag gaaagccgac tgtaattgtt aactggttgc agtcacctgg aggcaccagg
     7381 caccgcatca acaaagttca tttgtaaaaa tggagataat tatgattgca catcacttcg
     7441 gaactgatga aataccacgt cagtgtgtga ctcctggcga ttatgttctt catgaaggcc
     7501 ggacatatat tgcctcggca aacaatatta aaaagcgaaa actatatatt cgtaacctga
     7561 ccacaaaaac attcattact gaccgcatga ttaaagtctt cctcggtcgt gatggtttac
     7621 ctgtaaaggc ggagtcatgg tgatgactaa gaaaataaaa tgtgcttacc acctttgcaa
     7681 aaaagacgtt gaagaaagca aagctattga aagaatgctt cacttcatgc acgggatttt
     7741 atcaaaagac gaaccgagaa aatattgcag tgaagcttgt gccgaaaaag accagatggc
     7801 acatgaactt taattaattg actattcgaa actgaattta tgccagaaat ggcaggtatt
     7861 cgctcaacct taattaagga gaaaaacatg attaccaatt atgaagccac tgttgtaact
     7921 accgatgaca ttgttcacga ggtgaatctg gaaggaaagc gcattggcta cgtaattaaa
     7981 acagaaaata aagaagcccc attcactgtg gttgatatcg atggtccatc aggcaacgta
     8041 aaaacacttg atgaaggtgt caaaaaaatg tgcctggtgc atatcggaaa gaatctgccc
     8101 gcagaaaaaa aagccgaatt tctggcaact ctaattgcaa tgaaattaaa aggtgaaatc
     8161 tgaaagaaat agcctgcgta tggcgcaggc tatgaacagt gtgtatccgg caagatcatt
     8221 cactgaacaa aacgaatttt aatctgagtt gaggttaaaa aacaatgagc acaaaaccac
     8281 tcttcctgtt acggaaagcg aaaaaatcat ccggtgaacc tgacgtcgtc ctgtgggcaa
     8341 gcaacgattt tgaatcgacc tgtgccactc tggactacct gatcgttaag tcaggtaaaa
     8401 aactgagcag ctattttaaa gctgttgcca cgaattttcc tgtcgttaat gacctgcccg
     8461 ctgaaggtga gatcgatttt acctggagtg aacgctatca actcagcaaa gactccatga
     8521 catgggaact aaaaccggga gcagcaccag acaacgctca ctatcaaggc aataccaacg
     8581 tcaacggcga agacatgact gagattgagg agaatatgct actcccaatt tctggccagg
     8641 aactgcccat tcgttggctt gctcaacacg gcagcgaaaa accggtaacg cacgtttcac
     8701 gcgacggact ccaggcatta cacattgctc gggctgaaga actaccggct gttactgccc
     8761 tggctgtttc ccacaaaacc agcctgctcg acccgctgga aattcgcgaa ctccacaaac
     8821 tggttcgtga cactgacaaa gttttcccta atcctggtaa ttcaaacctg ggactgataa
     8881 ctgctttttt cgaagcatac ctgaacgctg actacaccga tcgaggactg ctgacaaaag
     8941 agtggatgaa gggtaatcgt gtttcacaca tcactcgcac ggcttccggt gctaatgctg
     9001 gcggcggaaa cctcaccgat cgcggcgaag gtttcgtaca cgatctgacg tcactggcgc
     9061 gcgacgtagc cactggcgta ctggcccgtt caatggatct ggacatctat aaccttcatc
     9121 cggcacacgc taaacgcatt gaggaaatta tcgctgaaaa taaaccgccc ttttctgttt
     9181 tccgcgacaa attcatcacc atgcctggcg ggctggatta ttcccgcgcc atcgtggttg
     9241 cgtccgtaaa agaagcacca attgggatcg aggtcatccc cgcgcacgtc actgaatatc
     9301 tgaacaaagt actgactgaa accgatcatg ccaaccctga tccggaaatc gtggatattg
     9361 cctgcggtcg ctcctctgcc ccgatgccgc agcgagtaac agaagaagga aaacaggatg
     9421 atgaagaaaa accgcaacca tctggaacaa cggcagttga acagggagag gctgaaacaa
     9481 tggaaccgga cgcaactgaa catcatcagg acacgcagcc gctggatgct cagtcacagg
     9541 taaattctgt tgatgcgaaa tatcaggaac tgcgggcaga actccatgaa gcccggaaaa
     9601 acattccatc aaaaaatcct gtcgatgacg ataaattgct tgctgcatca cgtggtgaat
     9661 ttgttgacgg aattagcgac ccgaacgatc cgaaatgggt aaaggggatc cagactcgcg
     9721 attgtgtgta ccagaaccag ccagaaacgg aaaaaaccag cccagatatg aatcaacctg
     9781 agccagtagt gcaacaggaa ccggaaatag cctgcaatgc ctgcggccag actggcgggg
     9841 ataactgccc tgactgtggt gcggtgatgg gcgacgcaac ataccaggaa acattcgatg
     9901 aagagagtca ggttgaagct aaggaaaatg atccggagga aatggaaggc gctgaacatc
     9961 cgcacaatga gaatgctggc agcgatccgc atcgcgattg cagtgatgaa actggcgaag
    10021 tcgcagatcc cgtaatcgta gaagacatag agccaggtat ttattacgga atttcgaatg
    10081 agaattacca cgcgggtccc ggtatcagta agtctcagct cgatgacatt gctgatactc
    10141 cggcactata tttgtggcgt aaaaatgccc ccgtggacac cacaaagaca aaaacgctcg
    10201 atttaggaac tgctttccac tgccgggtac ttgaaccgga agaattcagt aaccgcttta
    10261 tcgtagcacc tgaatttaac cgccgtacaa acgccggaaa agaagaagag aaagcgtttc
    10321 tgatggaatg cgcaagcaca ggaaaaacgg ttatcactgc ggaagaaggc cggaaaattg
    10381 aactcatgta tcaaagcgtt atggctttgc cgctggggca atggcttgtt gaaagcgccg
    10441 gacacgctga atcatcaatt tactgggaag atcctgaaac aggaattttg tgtcggtgcc
    10501 gtccggacaa aattatccct gaatttcact ggatcatgga cgtgaaaact acggcggata
    10561 ttcaacgatt caaaaccgct tattacgact accgctatca cgttcaggat gcattctaca
    10621 gtgacggtta tgaagcacag tttggagtgc agccaacttt cgtttttctg gttgccagca
    10681 caactattga atgcggacgt tatccggttg aaattttcat gatgggcgaa gaagcaaaac
    10741 tggcaggtca acaggaatat caccgcaatc tgcgaaccct gtctgactgc ctgaataccg
    10801 atgaatggcc agctattaag acattatcac tgccccgctg ggctaaggaa tatgcaaatg
    10861 actaagcaac caccaatcgc aaaagccgat ctgcaaaaaa ctcagggaaa ccgtgcacca
    10921 gcagcagtta aaaatagcga cgtgattagt tttattaacc agccatcaat gaaagagcaa
    10981 ctggcagcag ctcttccacg ccatatgacg gctgaacgta tgatccgtat cgccaccaca
    11041 gaaattcgta aagttccggc gttaggaaac tgtgacacta tgagttttgt cagtgcgatc
    11101 gtacagtgtt cacagctcgg acttgagcca ggtagcgccc tcggtcatgc atatttactg
    11161 ccttttggta ataaaaacga aaagagcggt aaaaagaacg ttcagctaat cattggctat
    11221 cgcggcatga ttgatctggc tcgccgttct ggtcaaatcg ccagcctgtc agcccgtgtt
    11281 gtccgtgaag gtgacgagtt tagcttcgaa tttggccttg atgaaaagtt aatacaccgc
    11341 ccgggagaaa acgaagatgc cccggttacc cacgtctatg ctgtcgcaag actgaaagac
    11401 ggaggtactc agtttgaagt tatgacgcgc aaacagattg agctggtgcg cagcctgagt
    11461 aaagctggta ataacgggcc gtgggtaact cactgggaag aaatggcaaa gaaaacggct
    11521 attcgtcgcc tgttcaaata tttgcccgta tcaattgaga tccagcgtgc agtatcaatg
    11581 gatgaaaagg aaccactgac aatcgatcct gcagattcct ctgtattaac cggggaatac
    11641 agtgtaatcg ataattcaga ggaataattc agcctggcgg tgtaatgcac cgccaacttg
    11701 aaatattttt tatgagaaaa attatgagat atgacaatgt taaaccatgt ccattttgtg
    11761 gttgtccatc agtaacggtg aaagccattt caggatatta ccgagcgaag tgtaacggat
    11821 gcgaatcccg aaccggttat ggtggaagtg aaaaagaagc actcgaaaga tggaataaac
    11881 gaaccactgg aaataataat ggaggtgttc atgtataaaa ttaccgccac tattgaaaag
    11941 gaaggtggca ctcctactaa ctggacaaga tattcaaaat ctaaactaac gaaatcagaa
    12001 tgcgaaaaaa tgctctcagg taaaaaagaa gcaggcgttt ccagagagca gaaagtaaaa
    12061 ctgataaatt ttaattgcga gaaacttcag tcctcgagaa ttgcattgta ttcaaattaa
    12121 aacttcatag ctgattatta ataatcaaca tcgggcgtca atttcagtct aacattggcg
    12181 cctgccagag gtgatgcgat ggcacaagta atctttaatg aagagtggat ggttgaatac
    12241 ggcctgatgc ttcgcactgg tctgggggcc agacaaattg aagcataccg ccagaactgt
    12301 tgggtggagg gcttccactt caaacgagta tctcctttag gtaagccaga cagcaaacga
    12361 gggattatct ggtacaacta tccaaagata aatcagttta tcaaagactc atgatatgtc
    12421 taaattacca acaggtgtcg agattagagg tagatacatt cgcatctggt tcatgtttcg
    12481 aggaaaacga tgtcgggaaa cattaaaagg ctgggagatt acaaacagta atattaaaaa
    12541 ggccggaaat ttaagagcgc tgatagttca tgaaataaac tccggtgaat ttgagtattt
    12601 aagacgtttt ccccagtcca gcactggggc aaaaatggtg acaacgagag tcataaaaac
    12661 gttcggagag ctttgtgata tctggacaaa aattaaagag acagagttaa caacaaacac
    12721 aatgaagaaa acgaaatcac aattaaaaac actcagaata ataatttgtg aaagtacccc
    12781 gatatcacat attcgttata gcgatatctt aaactaccgg aatgaactgc tgcatggaga
    12841 aacgctttac ctggataatc caagatccaa caaaaaagga agaaccgtgc gcacagttga
    12901 taactatatc gccctgctct gttcgctgtt gcgttttgcg tatcagtcgg gatttatatc
    12961 aaccaaacca tttgaaggag taaaaaaatt acagcgaaac agaataaagc ctgatccgtt
    13021 atctaaaaca gaattcaatg cattaatgga aagtgaaaaa ggacagagcc agaacttgtg
    13081 gaaatttgcc gtttactcag gacttcgtca cggggaactg gcagctctgg cgtgggagga
    13141 tgtggatctc gaaaagggaa tagtgaatgt cagaagaaac ctgacgatac ttgatatgtt
    13201 cggtccccca aaaacaaatg ccgggatccg aacagtaaca ctactgcagc ctgctcttga
    13261 agcactgaag gagcaataca aactgaccgg gcatcatcgc aaaagcgaaa tcacctttta
    13321 tcatcgggag tacggcagaa ccgaaaagca aaaactgcat tttgttttca tgcccagggt
    13381 gtgtaacgga aaacaaaaac cttattactc ggtaagcagt ttgggggcaa ggtggaatgc
    13441 agcagtaaaa cgtgctggta ttcgccgccg taatccgtac catacgcggc atacttttgc
    13501 ctgctggctg ttgacggcag gagcgaaccc ggcatttata gccagccaaa tggggcatga
    13561 aactgcgcag atggtgtatg aaatttacgg tatgtggatt gatgacatga acgacgaaca
    13621 gatagccatg ttgaatgcgc ggttatcgta gttgcaaagt ttgcccccaa tttgccccat
    13681 ttagtaccag agaactgaaa taatgcaaga aaatcaacaa attacaaaga aagaacaata
    13741 caacctgaac aaattacaaa aacgtctgcg tcgtaacgtg ggcgaagcca ttgctgactt
    13801 caatatgatt gaagaaggcg atcgcatcat ggtttgcctc tccgggggta aagacagcta
    13861 taccatgctg gaaattctgc gcaatttgca gcaaagcgcg ccaatcaatt tttcgctggt
    13921 ggctgttaac ctcgatcaaa agcaaccggg cttcccggaa cacgttctgc ccgagtatct
    13981 tgaaaagctg ggcgttgagt acaagattgt ggaagagaac acttacggta tcgtgaaaga
    14041 gaagattccg gagggcaaaa ccacttgctc actgtgttct cgtcttcgtc gcggtatcct
    14101 ttatcgcacc gcaacggaac tgggggcgac gaagatcgcg ctgggtcacc atcgtgacga
    14161 tatcctgcaa acgttgttct taaatatgtt ctacggcggc aagatgaaag gtatgcctcc
    14221 gaaactgatg agcgatgatg gcaaacatat tgtgattcgt ccgctggcct actgccgcga
    14281 gaaagatatt cagcgctttg ccgatgcaaa agcgttcccg attattccgt gcaacctgtg
    14341 cggttcacag cctaacctgc aacgtcaggt gattgctgac atgttgcgtg actgggataa
    14401 acgttatcca gggcgtatcg agacgatgtt cagcgcgatg cagaatgtgg tgccgtcgca
    14461 tctgtgcgat accaacctgt tcgatttcaa aggtattacc cacggttctg aagtggttaa
    14521 cgggggtgat ctggcgtttg atcgcgaaga gatccctcta caaccggcgg gttggcagcc
    14581 tgaagaagat gaaaatcagt tggatgagtt acggctgaat gtggttgaag tgaaataacc
    14641 cggatagcgc ccgatgcgca agcttaccgg gctactctta tggaggccgg ataagacgcg
    14701 gccagcgtcg catccggcaa tcccgaagaa gatgtttact cttgcaccct gcgattcaac
    14761 atttcattat tttaataacc gcacccggca cgtttttcct ttaatcttcc cgccctgtaa
    14821 ctgtttccat gctttatgag caacagcctg acgcaccgcg acatagacat gcgccggatg
    14881 cacggcgatt ttgccaatat ctgcgccatc aagcccgata tctcctgtca gcgcacctaa
    14941 tacatcaccc gggcgcattt tggctttttt cccgccatcg atacataacg ttgccatttc
    15001 tgcttccagc ggcacaatgg aactattaac tggcggcgtt tgccagttaa gttttatctg
    15061 caacatgtca gaaatgatat tggcccgctg tgcttcttcc ggggcacaga aactgatcgc
    15121 cagaccgcta tttcctgcac gagctgtacg accgatgcga tgtacatgaa cttcagggtc
    15181 ccacgccagc tcaaagttca ccaccagctc aagcgatttt atatccagac cacgcgcagc
    15241 aacatcagtc gcgaccagta cacgggcgct accgttagca aaacgtacca gggtctgatc
    15301 gcgatcgcgt tgctccagat cgccgtgtaa cgacaatgca ctttgcccta cttcattcag
    15361 cgcgtcgcag acagactggc aatctttttt ggtattgcaa aacaccacgc aagaggatgg
    15421 ctgatgcaag cttaataacc gttgcaacag aggaattttg cctttgctgg atgtctcata
    15481 aaattgttgt tcaataggtg gcagagcatc tgttgagtca atttcaatcg ctaaaggatc
    15541 gcgttgcact cttccgctga ttgcggcgat ggcttccggc caggttgccg aaaacagaag
    15601 cgtctgtcga gatgctggcg caaaacggat gacatcatca atggcatcgc taaatcccat
    15661 atccagcatg cggtcggcct catccatcac cagcgtattc aacgcatcca gtgataccgt
    15721 gcctttttgc agatgatcca gcatacggcc cggcgttgcc acgataatat gcggcgcatg
    15781 ttgcaacgaa tcacgctgca taccgaacgg ttgaccaccg cacaacgtca aaattttggt
    15841 atttggcaga aaacgcgcca gccgacgcaa ttcacctgcc acctgatccg ccagttcacg
    15901 cgtaggacac agcactaaag cctgggtttg aaatagcgac gcatcaattt gctgtaacaa
    15961 gccgaggcca aaagccgccg ttttgccgct gccggttttc gcctgcacgc gaacatcttt
    16021 tccggcaagg atcgtcggaa gcgcggcggc ctgcaccggc gtcatggtta aataacccaa
    16081 ctcattaagg ttcgtgagtt gggcgggagg caaaacattc agggtagaaa aagcggacac
    16141 aatttattct cgtggtcatc gacgcaaagt cagcaggcgc gtatcctcgc agatctacgc
    16201 tcacgatgcg acaatttaat cggttcttcg tcgggtggtg ggtcaggcat gggttgcggg
    16261 cgagggatcg gatcgggcac tggaacagga tcgccaggaa tcggttcagg gacaggaatt
    16321 tgcaaataaa taagtgtcgt catatttccc tccggtcatt gggtggactc ttaaagggta
    16381 gacgctgaca aataacaggc aaaaaaaagc cgactcatca aagtcggcgt cgtacgaatc
    16441 aattgtgcta tgcagtaatt caaaaaagga agtaagacaa tatggagcgc aacgcccatc
    16501 gcttgacgtt gcattcacct gcaagagaga tattgccctg aatgggtaga gagtttattg
    16561 acttcgctca aactttgcgg cgtttttgta tacagacagc cgggaaaatt gcttttgtta
    16621 caaccattta ctacgatgca accataaagc aacaccacca ataagaacaa ctaacagaat
    16681 acaaaaaatt gaaaatccga attgccaccc gccgcctggg atcccaccaa ggttgacgcc
    16741 aaataacccg gtcagaaagg tactgggtaa aaagaccatt gccatcaacg acattgtata
    16801 ggtacgacgc gctaaatttt cctgcatcac ctgagcgatt tcatcagcca tcacgccagt
    16861 ccgtgctata caggcgtcga tttcgtcaag accgcgccca aggcgatcgg caatatcctg
    16921 catccgacgg cgttggtcat cgctcatcca cggcaaacgt tcactggcaa gacgagcata
    16981 aacatcacgt tgcggtgcca tatagcgacg catcacaatt aattgtttgc gcagcagagc
    17041 caggaatcca cgcggtggaa tttgctgatc aaggagatta tcttcaaggt cgataatttt
    17101 atcgtgcagc tgctcgataa attcgctgga atgatcggtc aacgcatcac atacatccac
    17161 cagccatccc ccgcaatcgg tcggacccgt gccctcttcc agatcgctta ccacatcgtc
    17221 cagcgccagc actttgcgtt gtcgggtcga aacaattaac cgcccgtcca tatatacacg
    17281 catggcgacc agttgatcgg ggcgttcatc ggtgctgccg tttatacagc gcaatgtaat
    17341 cagcgtacct tcaccgagac ggctgactcg gggccgtgtg ctctcgcccg ccagcgcatc
    17401 acgtacgtta ttgggaagca gcggtgttgt cgccagccat tgggcgctat catggtgtac
    17461 ataattaagg tggagccagc agggatgcgc ttcatcaatc acatctgtat tttccagcgg
    17521 tttaacgccg cctctaccat ccagcatcca ggcaaatact gcatccggga cattaacgtc
    17581 cgatccctta atcgcttcca cagtgcctcc atcatcaacg cattattttg tagtctagcc
    17641 ttctggccct gttacgcaac atctcatcac cccattaccc tgaaatgatt aataaaattc
    17701 tgtctaaatt gaatacaaaa agcaaaatgc ttttccatat acaaaccgtg tgaagtgtta
    17761 aatagcgtct atcattatca gaattatctg atcatatgac gtggcttttt tgcgatcgga
    17821 tagcaacaaa aattgataaa aataacggga tctcaatgat tacgcacaac ttcaataccc
    17881 tggacttact caccagtcct gtctggatcg tttcgccctt tgaggaacag ttaatttatg
    17941 ccaacagcgc ggcgcgactg ttgatgcaag acctcacgtt tagtcagcta cgaaccggtc
    18001 cctattccgt ctcctcacaa aaagaactgc cgaaatacct ctccgatctg caaaaccaac
    18061 acgatattat cgaaatcctc accgttcagc gcaatgaaga ggaaacagca ttgagctgtc
    18121 ggcttgtttt gcgaaagctg acagaagcag aaccggtgat tattttcgaa ggtatcgaag
    18181 caccggcaac gctgggttta aaagccagtc gctcggcaaa ttatcagcgc aaaaaacaag
    18241 gtttttatgc gcgctttttt ctgactaact ctgcaccaat gttgttgatt gacccctcac
    18301 gagatggcca aatcgtcgat gctaacctcg ccgcgctcaa tttctatggc tataaccatg
    18361 aaacgatgtg ccagaaacat acttgggaaa taaatatgct cgggcgacgc gtcatgccta
    18421 tcatgcatga aatttcgcat ttacccggtg gccataagcc tttgaatttt gttcataaac
    18481 tggcggatgg ttcgactcgt catgtgcaga cctatgccgg tccgattgaa atttatggcg
    18541 acaagctcat gttatgtatt gtgcatgata ttactgagca aaaacggctg gaggagcagc
    18601 tggaacatgc tgcccaccat gacgcgatga ccggattact gaatcggcga cagttttatc
    18661 acattaccga gccaggccaa atgcagcacc tcgccatcgc tcaggattac agcttattgc
    18721 tcatcgacac cgatcgtttt aaacacatta acgatctcta tgggcattct aaaggtgatg
    18781 aggtgttatg cgccctcgcc cgcaccctcg aaagttgcgc tcgcaaaggc gatttggtgt
    18841 ttcgttgggg aggcgaagag tttgtcttat tgctaccaag aaccccactg gataccgcgc
    18901 tttcgctggc tgaaactatc cgcgtaagcg tggcaaaagt gagtatttcg ggcttaccac
    18961 gctttaccgt cagcattggt gtggcgcatc acgaaggaaa tgaaagcatc gatgaactgt
    19021 ttaaacgcgt tgatgatgct ttgtatcggg cgaaaaatga tggacgcaac cgcgtgctgg
    19081 cggcataagc cgcggatgcg tctcgagatc aacggctgcg cttagcgtgg cgctcccagt
    19141 tttcttgctt cgcctgcgcc gttttacgta gtgcgacgta acacgccccg ctgccgccat
    19201 gatgcggtag cgcggtgcaa tatgcctgaa catcatcaaa ttcggtcagc cagcgcgcca
    19261 catagctgcg gacaatattg gcatgcgatt tatcatcccg ccctttacca tgaataatca
    19321 gcacgttacg caaaccatcc gccatggctt gttgaatgaa actgaacatc attttgcggc
    19381 actcttccac cggctggcgc aaaagattca ggctcgcctg ttgcggatat ttaccactgc
    19441 gcagcttatc cagcacccca tgttgcaacc cttcccgccg aaactccagc ggctgactta
    19501 gcgggatgat gtcgagaaat ccggtggtga ggaaattatc aagttgcagc gtgtcgatac
    19561 gctgcggcgc acgttggtta cgcgttggat gccagtggac atctgtagca cgtttcagcg
    19621 gctggacatc ttccatggcg tcaagaaaca gcgatttgtc gtcaaggttc atgtaacctc
    19681 ctatgcacct tcacacgtcg tgattcagac gggttaattg ttcatactta ctgcatcgtt
    19741 cccttcacag attgaaacaa tactctactt catacctgtg agtatctgcg caggcggggc
    19801 attttatact agcctgttag cagtatcgaa cagtttagcc cgagtttaat tcagcacagg
    19861 ctccgatcag gatgatttat tcatagcagg aaccaacaaa aatgcgataa ataacagacc
    19921 actgattagc agaaaggtat ccgaaaatgc catagtttgc gcctccctca gcatcaactg
    19981 actcaataat ttggttgaag ccagtatttc cgatgtctgg tcacttccac tgcgattaaa
    20041 aaacaaagca cttctggaaa tgaagtcatt caccgtatgg ggaacagaaa ccattttttc
    20101 acccattctc gaaaaatgaa aattagttcg attattgaga atagagccac acagggcaat
    20161 accaatcgcg cccccaagat tacgtgttaa attaaataca ccagaggcta acttcagccg
    20221 ttcctttgga attcccccca gcgttaatgt tacgatgggg gccatcgcaa actgctgcga
    20281 aatccctcta attgcctgtg gaaacagtaa ctcctgccat ccccattcgt gggttatagg
    20341 ggtaaaaaga tacattgaaa acacaaatcc acccagcccc gccatcaaca gccatcgcag
    20401 atttattttt ttacttaacc agaagtaaaa gggaacagaa aataactgga aaatacctgt
    20461 tgtgcaaacg gcaaaaccta tctcttcggc atttaatccg cgaacctgcc ccaaaaacac
    20521 aggtattaaa tatacagtag agaatatccc aacccctcca gaaaaagaaa aaaaacatcc
    20581 cagcgtgaaa tttttatcct taaaagcatg aagatccatt atcggattgg atattgtaag
    20641 tgtacgcgca gcaaataaaa taaaactcac caatgccaga actgatgtca gcaaaatagt
    20701 attatcatcc agccatcccc agcgcgctcc ttcttcaagg gtatattcca gacatcccag
    20761 tgtcatggcc aacaggataa tactcggata atcagcaact ttcagtaaag agagatcagg
    20821 tttatcaaaa ttaaccagaa aaggaatact caaaactaaa tatatgccag gtaatatatt
    20881 gatataaaat agccatcgcc agtcgaggtt atcagtgatc cagcccccca gagttggccc
    20941 tagcgtcggt gacagtgagg ccagcgcact gactaccgca gcagccaacc ccaactcttt
    21001 accctggtaa taaataaacg ccgtcgtgaa aactaaaggg atcatggatg cgccagccgc
    21061 tccctgtaaa gcacgaaaca aaatcatgat ctgaatattc catgcaagtc cacacgcgat
    21121 actcattaaa gtaaatattc ccgcggatag agtaaaaagc caacgagtgg aaaatacccg
    21181 tgacagccac ccagagagcg gaataacaat aatctcagca attaaatagc tggtctgaag
    21241 ccaggccatt tcgtctttac ctgcggacag accaccacct atttcattta gggatgacga
    21301 tacaatttga atatcaagct gagcaataaa taatcccata cacatactgg cgaaagcaat
    21361 atgtttacgc atagacatca tctctcatcg acactgacgg tgacggacag tcccgggcga
    21421 agaagttgct ttaattcctt tgcgtcgtca aatgcaatcc gtaccgggac acgctgaaca
    21481 attttggtga aattgccagt ggcattttct atcggaatca agctgaaact ggctcctgtc
    21541 gctggggaaa ggctttcaat gtgaccgtga aacacttccc ctttgagaat atcagcacgg
    21601 atctcagcct tcattcctgg cttcattccc gctatttggt tttctttata atttgcatca
    21661 acccacaact cactgactgg tacaagtgaa actaacgatg tgccgccttc cacccaactg
    21721 cctgtatggg cgctgcggtt tgcgacaatg ccatcaatcg gcgcacgaac aagggtatat
    21781 tccagatcca gtagtgcctg agtcaaagcg gcctgaactt cttcgatact cgcacgtacg
    21841 ttttcttcct gagcagataa aactgccagc cgttgcttct ccaccaggaa atcgttcgcg
    21901 gctttgcgct cctttgctgc gacctgctgg tagtcaaacc tggcgttatc aataatttgc
    21961 tgcgatattg ccgctgattg tacgagtttt tcataacgtt cagtatctcg caggcgcttt
    22021 tgctcctcat gttttactgc ctgccatgtt tcactggcac tttgaattat cgactgctgc
    22081 atcgtaatag tggcttgaat gctttcgaga ttggcttttc tactttttat ttttgcctca
    22141 agcctggcga catttgcacg ataatcccga tcatcgagac gcaggataat atcgcctttt
    22201 tttacgctct gattatcacg cacctctata gcagaaatat aaccagaaac tttacttgcc
    22261 accgtggtaa tattaccacc aatgtaagca tcatccgtcg tctgtataaa tcttcctttg
    22321 cttacccacc agataaaaaa gacaacgcca gccaaaataa taccgatcgc tactacccca
    22381 ataagctgtt ttttagaaat tatcatcact ctttacaccg tatatatccg gaaacttgca
    22441 ttcgccatat ttaaaattta acatggctta tttgatgatt tctgacaaaa tacctactgt
    22501 aaatgacaat cacactgcaa cccaaagcat caccagggca tcacattatc ggactggatt
    22561 cggaacatct gaatggaggc acagttccct ggcataaaca tctctatgcc cagttgcttt
    22621 acccggctga aggagtcgtt cgtgtatggg ctggagaaag cgtctggcta gtgcatgcca
    22681 gcagcgcgct ttggctgccc ccacaaatgc cgcataagtt tgttgcgaca ggcaacgtac
    22741 ttttaaaaac cgtactggtt tcagaagcgg aaagtgaaac gctgggaaaa gtttgcttca
    22801 tgacgggtat ttcacccctg ttaagagaat tactaattgc aattaaccaa cttccaccgt
    22861 cgcaaagtac cacagataaa caacagttac gtttttcagc cctcgagaca ctgatacttc
    22921 aggaaataaa aatgggggtg aaaatgtcac ttgaactccc ttggccaaat gatgagcggc
    22981 tacagcagct ttgtgagaat ttgctaaaca accaagggta tctgccaaca ctggataatc
    23041 tagcggataa aattaacgtc agtagccgta cactcatgcg cttatttgtt aaagagacgg
    23101 gattaacatt tcgccactgg gttcagcaaa tgcacgttat ttctgcagtg acgcttcttg
    23161 atgatggata ttcgttgacc aaaattgccc atcgtctggg ctatgcctca gctgaatcgt
    23221 tcggcaatat gtttaagcgt cgaacgggct actcgcccgg taaatttact cgtcgattaa
    23281 cgatgcatga ttatgcaatc acaaggcaaa tgatttaact tatattttcg tgaaatctgt
    23341 cactgaagaa aattggcaac ggatgggtaa aaccgttata acactgtcac cggtcaccag
    23401 gaccccaggc cggatttaag acgaggatgc actgctgtgt gtactgtaga gtctggcgga
    23461 tgtcgacagg ctctattttt ttattcattt tttagctctg taaataacca cattctcgcc
    23521 gggattgggg aatgagtgac gagatcagtg gtgtctggcg gctatcacgc cacagaatca
    23581 aataacagat agcctacggt aaaatttcgc tgactggaat tatcccaccc tcgatccatg
    23641 tgatggcttg ccctcatttc ttcaggcaga aaaatcaaaa aatcgcttta tgctatcaga
    23701 ctgcgacaaa ccagacgcga aactgggtac ttactattcg ttagtcttgc cctatccgct
    23761 tatctttttg gtggtatggc tgctgatgtt gctggcgtgg tatcttgttg gtctgccgat
    23821 aggtccgggg atttacccac gtttgtctta agagagaacg gatgctgaga ttacttgaag
    23881 aaaaaattgc cacgccactg ggtccactat gggtgatttg cgatgagcaa tttcgcctgc
    23941 gggcggttga atgggaagag tacagcgaac gcatggtgca gctgctggac atccattatc
    24001 gcaaagaagg ctatgagcgc atttctgccg ccaacccagg tggtttaagc gacaagcttc
    24061 gtgaatattt tgccggtaat cttagcatta ttgatacgct tcccactgcc acggggggga
    24121 cgccatttca gcgcgaagtc tggaaaacac tgcgcaccat cccctgcggg caggtaatgc
    24181 attatggcca actggctgaa caattaggcc gccctggcgc agcgcgtgcc gtaggtgcgg
    24241 caaatggatc gaatcccatc agcatcgtcg taccttgcca tcgggttatt ggccgaaacg
    24301 gcaccatgac cggatatgca ggcggagttc agcgaaaaga gtggttattg cgccacgaag
    24361 gttatctttt gctgtaaaca ttaaacaatt tgtgccagct tgttcacact tttatataaa
    24421 gttaccctta acaacttaag ggttttcaaa tagatagaca tatatttaca tctaatatcg
    24481 gaattctctg ctgttaaggt ttgcttagac ttacttgctc cctaaaaaga tgttaaaatt
    24541 gacaaatatc aattacggct tgagcagacc tatgatcccg gaaaagcgaa ttatacggcg
    24601 cattcagtct ggcggttgtg ctatccattg ccaggattgc agcatcagcc agctttgcat
    24661 cccgttcaca ctcaacgaac atgagcttga tcagcttgat aatatcattg agcggaagaa
    24721 gcctattcag aaaggccaga cgctgtttaa ggctggtgat gaacttaaat cgctttatgc
    24781 catccgctcc ggtacgatta aaagttatac catcactgag caaggcgacg agcaaatcac
    24841 tggtttccat ttagcaggcg acctggtggg atttgacgcc atcggcagcg gccatcaccc
    24901 gagcttcgcg caggcgctgg aaacctcgat ggtatgtgaa atcccgttcg aaacgctgga
    24961 cgatttgtcc ggtaaaatgc cgaatctgcg tcagcagatg atgcgtctga tgagcggtga
    25021 aatcaaaggc gatcaggaca tgatcctgct gttgtcgaag aaaaatgccg aggaacgtct
    25081 ggctgcattc atctacaacc tgtcccgtcg ttttgcccaa cgcggcttct cccctcgtga
    25141 attccgcctg acgatgactc gtggcgatat tggtaactat ctgggcctga cggtagaaac
    25201 catcagccgt ctgctgggtc gcttccagaa aagcggtatg ctggcagtca aaggtaaata
    25261 catcaccatc gaaaataacg atgcgctggc ccagcttgct ggtcatacgc gtaacgttgc
    25321 ctgatttttc cgcataactc actatccttc tgtcatatca ttaaattttt ctgatttatt
    25381 gatctggcag aaggttcatc actgtttcat tcaccagata tgggttaatc ttttaattac
    25441 aaactgcgtt gacagttgtt gtaaggagac cctgtatggc tatgtatcag aacatgctcg
    25501 ttgttatcga tcctaaccag gacgaccaac cagcattgcg gcgagctgtt tatttacatc
    25561 aacggattgg tggcaaaatt aaagcctttt tgccgatcta tgacttctca tacgaaatga
    25621 ccaccctgct ctctccggac gaacgtaccg ctatgcgtca gggcgtcatc agccagcgta
    25681 cagcctggat ccacgagcag gcaaaatatt atctcaatgc tggcgttccc attgaaatta
    25741 aagtggtctg gcataaccgt cctttcgaag ccatcattca ggaagtgatc agcggcgggc
    25801 acgatttggt gctaaaaatg gcgcaccaac atgaccgtct ggaagcggtg atttttacgc
    25861 caacggactg gcatctgtta cgcaaatgcc caagcccggt gtggatggtg aaagaccagc
    25921 cgtggccgga agggggtaag gcgctggtgg cggtgaatct cgccagtgaa gagccgtatc
    25981 ataatgcgct caatgaaaaa ctggtcaaag agacgatcga actggcggaa caagtcaacc
    26041 ataccgaagt tcatctggtt ggcgcttatc cggtaacgcc aattaatatc gcgattgaac
    26101 tgccggaatt tgatccgagc gtttataacg atgccattcg tgggcaacat ttgctggcaa
    26161 tgaaagccct gcggcagaaa ttcggcatta atgaaaacat gacgcacgta gaaaaaggtc
    26221 tgccagaaga ggtgattcct gatttggcgg agcatttaca ggcggggatt gtggttctgg
    26281 gcacggtcgg acgcaccggt atttcagcgg cattcctcgg caacacggcg gaacaggtga
    26341 ttgatcatct tcgctgcgac ctgctggtca ttaaacctga ccagtatcag acacccgttg
    26401 aactggatga cgaagaagac gattaacgac tcttcaataa ctgacaaggc ccgagttatc
    26461 gggcctattt tttacttact gctcttcgcc gccaagaaaa taaattccca acgggattgc
    26521 cagcaacaca gtaaagacca ggctataaac aaaaatcatc gatgactgaa tcacatacat
    26581 tgatgtcgtc cactcagaca gcggaatatt gtactgttct ataacgccgc cgatggtcgc
    26641 gcgagtcaca accgacgccg caataatgaa aaccgccaac aaaccaaaga gaaatttctt
    26701 ccctttcgct gacttcagtt tcgccataat catgatgtaa tccttgtgca gacaaatttc
    26761 ttgttaccta aacgtaacaa taccatgaca tttcaccgtt gtctgctctg aaaacctcac
    26821 ctcttagctg gatttacaat caacgcattg attagttagc taatgttgct acttccttcc
    26881 ctgactattg atgatctgaa tgaattgtcg cggtaaaata gccccgccag gggtttccgg
    26941 catgacgaca attatctgga ggagcctgat aaaacaatga tcgctgaact gtttacaaat
    27001 aatgcgctaa atctggtcat tattttcggt agctgcgcag cattgattct gatgagcttt
    27061 tggtttcgcc gcggaaatcg taaaagaaaa ggttttttat tccatgcggt gcaattttta
    27121 atctacacca taattatcag tgctgttggt agcatcatta attatgttat agaaaactat
    27181 aaactcaaat ttatcacccc aggcgttatc gattttatct gtacgtccct gattgcggct
    27241 attctgacga ttaagctatt tctgctgatt aatcagtttg aaaaacagca gattaaaaaa
    27301 ggtcgcgata tcaccagtgc gcggattatg tcgcgtatca tcaaaatcac cattattgtg
    27361 gtgcttgttc tgctttatgg cgaacatttc ggcatgagcc tttctggctt gttgaccttt
    27421 ggtggtattg gtggtctggc tgtcggtatg gccggtaaag atattctgag taacttcttt
    27481 tccgggatta tgctctattt cgaccgtcct tttagtattg gcgactggat ccgttcaccg
    27541 gacagaaata tcgaaggtac agtagcggaa attggctggc gaattaccaa aattacgacc
    27601 tttgataatc gtccgttgta cgtaccgaac tcgctgtttt cgtcaatcag cgtagaaaac
    27661 ccaggacgaa tgaccaaccg gcgcattacc acgaccattg gtttacgtta tgaggatgcg
    27721 gcaaaagtgg gcgttattgt cgaagccgta cgtgagatgc tgaaaaatca cccggccatc
    27781 gaccagcgac aaaccttact ggtttatttc aaccagtttg ctgactcttc attgaatatt
    27841 atggtttatt gctttaccaa aaccacggta tgggctgaat ggcttgctgc acagcaagac
    27901 gtttatttga agattatcga tattgtacag tcacacggcg cggactttgc cttccccagc
    27961 cagacgctgt atatggataa cattacaccg ccggaccagg gtcgctaaat cagacatcac
    28021 tggcgttatc cacaagggta acgccagttc ccatcacatc aatgcttcac aatatacata
    28081 gtccgactgt acgccacatc ttccgggtta ttaatgggat aacctttcag ccacggcttg
    28141 attaatcgtc cattggtata ttgataaatt ggtgcaatcg gtgcttgctc catgaggatt
    28201 ttttctgccg cattgtaatc ggcattacgc gctttaacgg tattttccgt tgatgcctgg
    28261 gccagtactt tgtcgtatgc cggattgtta aagcgtgaaa tatttcctga atgcgttgac
    28321 gttaataatg tcaggaaagt ggagggttca ttataatccc ctacccacga ggctcggata
    28381 acatcaaaat tgccggtgtt acggctatcg atataggttt tccattcctg attttgcaat
    28441 ttaacatcta agccaaggtt ctttttccac attgatgcta cagcaatcgc aattttttga
    28501 tggttttctg aagtgttata caaaagcgtc agcttcagcg gtttttgcgg accataacca
    28561 gctgcgctca acaaagtttt tgcctgcgca ttcagttctt cctgactcat ttgttcaaac
    28621 ggcgaaggtt ccggcgtaaa tcctgcagta acatctggtg taaaatgcca cgctggcttt
    28681 tcgcccgttc ccaacacttt ttcggtcatc aggcggcgat ctatcgtcat acttaatgcc
    28741 aggcgaacac gctgatcggc cgtcggccct ttttgcgtgt taaacgcata ataataggtc
    28801 ccgagctgtg gcggcgtata aacctgcccc ggaatatcct tcaacagctt ctgatacata
    28861 tttttcggga aggattcggt gatatcaata tcccccgcga gataacgctt agtggctgcg
    28921 gattcctgat taattggcag gaaggtcact ttttgcagca ccgttttggc gttatcccaa
    28981 taatgggtat tcggtaccac gaccagtttt tcattgacta cgcgctcttt aagaacataa
    29041 gcgccattac cgatcagatt tccgggtttc gtccactctt tactgctttc tacgttggct
    29101 ttttgcaccg ggaagaaggc aaagttagcg gttaaattca caaaccacgg caacggttta
    29161 tcaagctgga ttttcaaagt ttgggcatca actgcggtga cgccaagctg gtcaggcgcg
    29221 gctttaccat caataatcgc ctgtgcgttg ttgattcccg ccagcgcggc aaaccatgca
    29281 aatggcgaca atgtttttgg gtccaccaga cgttgccagc tgtagacaaa atcttgcgcc
    29341 gttaccggtg tgccatccgc ccattttgcg ttatcgcgca gggtaaaagt ccagatacgg
    29401 ttgtcattac ttttccactg agtcgcaacg ccggggacaa tctccccttt ctcgttctga
    29461 ttcaccagtc cttcaaacag atcgcgaatg acctgaatct ctggcaggcc cacggcttta
    29521 gcgggatcca gcgacgcagg ctcatcttta atgtggcgca ccagctcctg cttctctgcc
    29581 agtactgtgc cgctcggaac ttcagcagca tacgaaagag aaatgctgct gaccaacagc
    29641 gcacaacacg tgactgaaac agagtgcttc ataagtttcc ctctaaccga cattaaaatt
    29701 gatgcgtaat tatttgtttc gccttaaaga aatgcaaaca acttccatca aaaaagtgac
    29761 aaaggaagca tcaaatgttt taagtatcaa tatatcaaat cagatttagt gtaaataata
    29821 gtctccaaaa tgcggtaaag cacatagaac tccgaacact acgttcgggc ttcgcacgtt
    29881 caaacccgtt ttcggtatca taatcagcat ctgtcgccat tgtacgaaga ataaaaaata
    29941 accaaaacgt tgaaaggaac tgagaatacg ttaagagaaa aattaaatta atgtatcagc
    30001 aagtagtgct aaacagcacc tttatacttc agcctatcta taaataatga gaatgccgag
    30061 ccagcatgtc gacggtgtgg atagtacagg tgataaccgg gtaaatctgg tgtgaattta
    30121 tccaaaacac gtatcagttt tttttcttta atagcccgct caacctgatc ataaggtaga
    30181 tacgccaatc catgcccatc aattgcagca tcaatgatca ggtctatcgt attcagtaaa
    30241 agctgacctt ccatgcgaac acgaacttca cgtccaccgc gtattaatct ccagcgattt
    30301 gctgtacccg atgtgggaag atacaaatta attgcctgat gatctattaa ttgtgacact
    30361 gaggttggag cacttcgacg agaaaaataa tccggtgagc caacaatagc cattggtata
    30421 tctggcccga ttcgaatggc gatcatatct ttatccatct ccccacccag acggacgcct
    30481 gcatcaaaac gttcagaaac gacatcggtc aaaccataat caatggtaag ctggatatca
    30541 atttcaggat gcgatttcag gaatgtgcgc atagctggta acaatatcgt ttttgctgca
    30601 tgttctacag tagtaatacg tattgtcccg gatgggcggt tctgcagatc gctcagggat
    30661 gtcagggcag aatctatgtc atgcaacata gggccaagaa cagacaaaag atgctcgccc
    30721 gcttcagtgg gtacaacgct gcgcgtggtt cgtgtcagaa gccgcaatcc caatcgttct
    30781 tctatacggc gcactatctg gcttaaagct gactgcgcca tgctcaggcg ggctgctgcg
    30841 cgagtgaagc tacgctcctc tgcaacgacg acaaacgcca tcagatcagc gatttcttca
    30901 cgcttcattc agtatctcca tacaatctat taatcattta aatgataggt ctaatcatga
    30961 ttttgggtct aatcaactta atgatcaata tctatagtga tatcaccaac cgtcaaaacg
    31021 aaccgacaac tccatgagga acggaatgat gaataataaa gtcagcttca ctaacagcaa
    31081 taatccaacc atctctttgt ctgcagtgat ctatttccct ccaaaatttg atgaaacccg
    31141 tcagtatccg gcaattgtgg tatctcaccc aggtgggggc gttaaagagc aaacggctgg
    31201 aacctatgcc gaaaaactgg cggaaaaggg atttgtcact attgcttatg acgcatctta
    31261 tcaaggtgaa agcggcggcg agccgcgtca gttagaaaac ccatacattc gtactgaaga
    31321 cgtcagtgcc gtgattgatt acctgacaac gctttcttat gtcgacaata gccgaattgg
    31381 tgcgatggga atttgtgccg gtgcaggata tacagccaat gctgcaattc aggatcgccg
    31441 catcaaggcc atcggtactg ttagtgcggt caatattggt tcgatgttcc gtaatggttg
    31501 ggaaaacaat gtgaaatcta tagatgccct accatacgtt gacgcgggtt caaacgcccg
    31561 aaccagcgat attagcagca gagaatacgc cactatgcca ctggcaccaa tgaaggagtc
    31621 cgatgcgccg aacgaagaac tgcgtcaggc ctgggagtat taccataccc ctcgcgcgca
    31681 gtatccaaca gcaccgggtt acgctacttt gcgcagcctt aaccagatta ttacctatga
    31741 tgcttaccat atggcggaag tgtacctgac tcagccgata cagattgtgg cgggcagcca
    31801 ggcagggagc aaatggatga gtgacgacct gtacgatcga gcatcaagcc aggataaacg
    31861 ctatcacatt gttgaaggcg caaaccatat ggatctttat gacggcaaag tctatgtggc
    31921 tgaagcaatt tcagtattag ctccgttctt tgaggaaact ctgtaatgaa acatattcaa
    31981 atccgtaata gtgatatggc ctggcatatt gctgccaata atctgggaaa agtttcatga
    32041 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
    32101 cagataaaca aaccattaaa caaactttat ttgcccgtca gccagcaaaa atccacaaac
    32161 cctatcccac taatagcaag attattatgg gggatgtact gaatcatgca gcactgaagc
    32221 aggccatgca gggacaggat gttgtgtatg ccaaccttac gggggaagat ttagatattc
    32281 aggcaaattc agtgatcgct gcgatgaaag ccggtgatgt taaacgtctg atctttgttc
    32341 tttccctcgg tatttatgat gaagttccag gaaaatttgg cgaatggaat aatgcagtta
    32401 ttggtgaacc cctaaaacca ttccgcaggg ctgcagatgc aattgaggct tctggactgg
    32461 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
    32521 cctcacgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
    32581 ttacagatat catcgataaa cctgagaaac acattggtga gaatatagga atcaatcaac
    32641 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagct accatcgtac
    32701 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
    32761 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
    32821 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
    32881 ataacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
    32941 aatacttcct gaaccagaga cgatcatgac cgtaactcgc ccacgcgccg aacgcggcgc
    33001 atttccgccc ggtacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
    33061 tccggcccct gcagccagtc acgaaagtgg tttgattctg gctggcactc acggtgatga
    33121 aaactcttcg atcgtcacgc tctcctgtgc tctgcggact ttgacaccgt ctttgcgccg
    33181 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ttagggttac gggccaatgc
    33241 taatggtgtg gatttaaacc gaaactttcc ggcggcgaac tggaaggaag gtgaaaccgt
    33301 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttgctgacag gtgacaaacc
    33361 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
    33421 ggtggtctca ttccacgatc cgctggcctg tattgaagat cccagacaca gcgaattagg
    33481 tgaatggctg gcccaggcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
    33541 tggctctttc ggtagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
    33601 acccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
    33661 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
    33721 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
    33781 gcgctaatgg cccgagaggt acacaacatg cagcccagca tcagacgaaa accttgtgca
    33841 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc aagcttaatg
    33901 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
    33961 tcagcacaaa tcggtaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcaggg
    34021 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
    34081 ccttctgcac gccaggattc atttgcgtca acgatcagcg tcgcatcggg cacagcggtg
    34141 cgaattgcca ccatccgctc actgataaga tggttatcca gctttacttt cagtaatttc
    34201 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgact
    34261 accgtctggg cagtggtgac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
    34321 tgctgctgtt ttcgcgccgc cagatcccac aatgcacaat ccagcgcatt acgtgctgcg
    34381 ccggcaggga ggattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
    34441 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
    34501 cattcaccgg tgcctttaat accttcttct tccagttcaa ccaccaccac gcgcgcttca
    34561 ctgcgacttc cccaagcaat cacaaacggg gtatgtaagg gccagccttc ctcgaatacc
    34621 ttaacggttc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
    34681 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
    34741 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
    34801 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
    34861 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
    34921 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac agagatcgac
    34981 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
    35041 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga attcctgcaa
    35101 gcctacggtg tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg
    35161 gttattgatg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
    35221 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
    35281 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccgtattccc
    35341 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
    35401 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacgactg gtgatttcgt
    35461 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca
    35521 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagtgtg cgatagatag
    35581 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagcaca gtattcaggt
    35641 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
    35701 cggttaacgg catgatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
    35761 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccaaat
    35821 tcttctgcgt agcgcaaatc acccttacat cgacatgcac ttcatgatct tcaccaactc
    35881 gacggaaagt accatcattg aggaaacgca gtaatttcgc ctgcatcctt ggcgacatct
    35941 cgcctatctc atctaacagc accgaaccac cgttcgcctg ctcaaagaac ccttttttac
    36001 cttccggagc atgaccaaac agttcactct cgaccgcatc ttccggtata gacgcgcagt
    36061 tcagcgccag gtaaggtttg cccgctcgtg ggcttgcctg atggcaggcg taggcaaaga
    36121 gatctttacc tgtaccggtg tcacccgtaa tcagcaacgg cgcgcttagc atcgccagtt
    36181 tctgcgcctg ttcgacaaca tgcttcattt tcgggctgac ggcgacaatt tgactgaagg
    36241 cactgacgtc ctgggcggcg acattctgca actgacggcc catacgaatc gtcgagcgca
    36301 acatcaccac cgcaccggtc agaacatgtt gatcattttc atcctgaaga taaacaggcg
    36361 taatctccat caggaaattc tgcccattaa taacaacatg ctcgttatgc gagtcttgcg
    36421 gttcgctttc cagccagcgt aaaaaattaa agccgttaat taattgcgcg gcggtatggt
    36481 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
    36541 ctttgctttt catatcaaca gaaagcacag gctcaggcaa cgcctccagt aacgcgctca
    36601 acgccagatg ctcacgttcg gaaggcatcc acggaacagt gcgcacatcg gtaacacctg
    36661 caatacggcg tatttcggcc atcagactgc tgaaactttc aaattccagt tcggcaaaat
    36721 tgaggtagat tcgcccaatg gggtcaatct caataccgcg taaatcaatg cctctcagca
    36781 ccagtagatc gagtaattcg cgggtcagac caagtcggtc ttcacaaaag acttccagac
    36841 gcatgggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
    36901 agcaggaagg tttctgtcaa caatgattga cacaaagacg gaaaaacggg cacttcggcg
    36961 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
    37021 gcagcgtctc tttcacctga ccgataagct gacgacggaa atccccgagg cgaggtttgt
    37081 catcttcaat ccacggcagc gggcggcaaa gttccatagc tttaatcccc agtcgtgccg
    37141 tcagaagtcc agcaccaatc ccctgagctg cccgggtaga caaacgcgca gcgagatctt
    37201 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggcg aaagcgatat
    37261 tcagcaatac cagcttaaac aggcgcaaac ggctgtaata tcccagttca atgccataca
    37321 gcgtggcgat gcgattaatc aaacgcagat tgcgccaggc gataaacgcc atatccacca
    37381 gcgccagagg gctgaccgca atcatcaacg ttgattccgc tgccgaacgg ctgatttcgc
    37441 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaaactg accacttcac
    37501 ggtcgttttg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgtgactgat
    37561 cgatgcccgc ctgctgtgcc agtttttcgc aaaatgcgcg gcctttaccc gtgccgtgac
    37621 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
    37681 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
    37741 atccacccag cgccacccag tcctgggttt gccaggcgtt cattgtccac tgtacaccct
    37801 gcccgacaac gcttgcgcca aacagagcca acccgcccat caccattttg cgccacaggc
    37861 tgcgtttcgg acgtaacgct gcgtccatca ccgcttcaac ttgcccctct tcttcctgcg
    37921 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
    37981 ccctgaattt tggattctga tcgacctcca gtggaccgtc gaaatcaata cgtggtttta
    38041 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcagcat caaggcgaat
    38101 atgcggcagc ggtttgtcga catccatcac ctgcggacga aacgcctcaa actggaagcc
    38161 ttgcttatcc cagaacgcct gaccgggcaa acgagcggga acttcgccag gataaacagt
    38221 gagcggtgcg ccatcgctga gtcgattacc gcgcagcgcc gggattttct caccgttaac
    38281 atcaataatg ccgctggtgg tcgcctgaac tgacgccagc cccaggcagt ccatgctgat
    38341 cccttcgaac gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
    38401 attagcgtgc tgatcgatgg tcacatggtc cgctttagtg gcagcaaaca atagcttatc
    38461 gataaccggc gaaaacaaac gccggaacag ggtacgctgc ccgtagtgga aactttgcat
    38521 cagctgcgtc agcgccagac gcatatcatt aaatgcctgt ggtccactgt tgagcggttg
    38581 caggcagtcc accagcacaa tctggcggtc aaaacgcagg aagtgatttt tatagaatcc
    38641 tttcaccacc ttctcgcagt aataattaaa acgctcgcgc aacattccgg cgttggtgtg
    38701 tttatcggcc tgcgccagtt tagactcgcc ccaggcatcg acatctggcc atgggaagaa
    38761 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
    38821 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacgcggcgg caatgtctgc
    38881 cagccggttt tcgtcggcag gcgctagcgg gtccagccct tcgcacatca ttcgccattt
    38941 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
    39001 atagtcctgc gccagcatcg gcaggtcgag caaccattcg ccggggtaat ccacaatctc
    39061 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gaatcgttcg atttatagcg
    39121 tagcgccagg cgaatttcgc tgacgccgcg cgttggcgtc ggccaggcgg gaggatcgcc
    39181 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
    39241 aatgcgtttt acgcccagca ggcgttcttc acgcaccgca cttaatagcg gcaaacgtgc
    39301 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
    39361 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
    39421 attaagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
    39481 aatcattcgt aaggatatga atgtgggggc caggccattg attatcaact gcccgtgttg
    39541 tcatcgacgg gcgaactcca taagatgtgc aacattcacg ttcggacgga gttcctgacc
    39601 tatgtcccct actatttatg atattgccag ggttgcaggc gtatcaaaat ccaccgtatc
    39661 acgcgtgctg aataagcaaa ccaatatctc cccggaagcg cgcgaaaaag tgttacgggc
    39721 cattgaagaa ttacagtatc aaccaaacaa gctggcacgc gcgctgacct cttcgggttt
    39781 tgatgccatt atggtgattt ctacccgttc gaccaaaact acggcgggta atccgttttt
    39841 ctcggaagtt ttacatgcca tcaccgccaa agctgaagaa gaaggtttcg acgtgatatt
    39901 gcagacgtcg cacaacctgg cagaagactt acaaaaatgc gaaagcaaaa ttaagcagaa
    39961 aatgattaaa ggcattatta tgctaagttc gccagcggat gagtcatttt ttgcccaact
    40021 cgataaatat gatattccgg tggtggtaat tggcaaagtt gaaggtcaat atgcccatgt
    40081 ttattctgtc gataccgata attttggcga cagcattgcg ttgaccgatg cgttaattga
    40141 aagtgggcat caaaatattg cctgcctgca tgctccgctt gatgtccatg tctcggttga
    40201 ccgcgttaat ggctataagc aaagtctggc tgcgcataat attgcagtgc gtgatgaatg
    40261 gattgttgac ggcggttata cccatgaaac agccttgcaa gccgcacggc aattattaag
    40321 ccagtcgccg ttgccagaag ccgtatttgc cactgacagc ctgaaattaa tgagcattta
    40381 tcgtgcggca gcagagaaaa atattgctat tccgcagcag ttagcggtgg tgggctatag
    40441 caatgaaacg ctgtcattta ttttaacgcc tgcaccaggc ggcatcgatg ttccgacgca
    40501 ggagttaggt caacaaagct gcgagttatt attccagcta attgccggaa aaccgtcacc
    40561 acaaaatatt accgttgcta cgcatatgtc gttgaaataa atgtatgccg gatttggcgc
    40621 tcgccccgca tccggcaatt tagcacaata tcagaacgag taattcaccc caacacccgc
    40681 ataatggaat ttatcgctgt cgccttcgtc gtgatcctgc cactcaaacg cgtattccag
    40741 cgaaacggaa agaccattct ggaaatcata accgtaaaat aaccccacac ggttaaaatc
    40801 atggccttca cgttcaatat catcctgcca gtcccagtta ctccagcgat ccagcccaat
    40861 gcgcgtatac ggcgtcaccg agtggttgcc gagcgtcagc ggcaaatagg cgcgaatttc
    40921 ttgcgtggaa aactcaccgt tattgcggct atcatccata ttgaaaccgc gctcgagata
    40981 atagttcact cgcaaggcaa ccgtttcgtt gaaggtatat tgcagacccg tttccgtttc
    41041 gacacgggta tcagcataac cggtcgtatt cagatcgttg gcaaatttat acatcgacag
    41101 ccaaccgttg aaacgtaaat cgtcagtcag tttcacatcc cagtctggcg caattttcca
    41161 gcgctgcata ttcgccgtgt ctttacccgg ttcatcaacg tagtgataac cataattacg
    41221 gaaaccacca gtcagaccga aactgaaatc atcgctttcg aggaactgat aatgtacctc
    41281 cagctccggg cgatcaaacc acgttccaag tttacccgcg ctataatcta ccggcccttc
    41341 ctgataatag gccagagaaa ttctccacgg cccgttggcg gcattaaaat agacagaagg
    41401 ctccgccagc ccatccatat cttcgccata accctcgacg ttttctattt cgtacatcgc
    41461 gccgatatta aagtgccagt cgttcttttc ctcggcctgt gcgcaggcca ttcccgcaca
    41521 catcaccagt gcggtacagg gtaataattt tttcattatt gttccttgta ttatcctggg
    41581 ggaatgcctt ccccctgtat ttagcgaatt gctatttccg tttctgcatc aaagaaatga
    41641 catttagtca tatcaaaatg aatagtgata ttttctcctg catgataatc atttaacgca
    41701 ccagcacgga ccaccagttc gtgcccccca accgtggtgt agagcataaa ttcagcaccg
    41761 gttaattctg ccacgctaat tttggcggaa atgttatttt cctcttgcgc ttgcggctga
    41821 atatcttccg ggcgtattcc catcacaatg ggcttatgca aactttcctg tgttttcaga
    41881 accgctaatt tctcctcggg aatggttaat ttaagcgttt ccgtaacgaa tttatcgcca
    41941 tcaatcgtgc cgcgaataaa attcattgca ggtgatccta taaatccggc aacaaacata
    42001 ttcgcgggtt ggttataaac cgttttcggc gcacctactt gctgaacaat cccatccttc
    42061 ataatcacaa tccgcgtcgc catggtcatc gcttcggtct gatcgtgggt cacgtagatc
    42121 atggtggtgt tcagtttctg atgcagcttg ctgatctctg cgcgcatttg cacgcgcagc
    42181 ttggcatcca ggttagagag cggctcatcc attaaaaaca cgcccgcttc gcgcacaatc
    42241 gcccgcccaa gcgccactcg ctgacgttgc ccgccggaaa gcgcgcccgg cttgcgtttc
    42301 aggtactcac gcaggccgag aatttgcgcc gcccagttca cccgctcatc aatcacctct
    42361 ctggcgattt tttgcatctt caggccaaac gccatgttgt cgtagaccgt catatgcgga
    42421 tacagcgcgt agttctggaa caccatcgcg atattgcgtg ctttggctgg aacgtcattc
    42481 attcgtttgc cgtcgatcaa cagatcaccg ccgctgatct cctcaagccc ggcaatcata
    42541 cgcagggtgg tcgatttacc gcagcccgac gggccgacaa acacgatgaa ctctttatcg
    42601 gcaatctcca ggctgaagtc cttcaccaca tgcacctggt tatcgtagat cttttgaata
    42661 tgttgtaacg aaagctgtgc catgttgatt cctttgctat acgttttgcc agaaggccga
    42721 taaccgcggc caggtcaggg agtccgttga gggcaacaat aattgcgccc cggttaagcc
    42781 cgcgccgatc cccaccgagc gcataccgct ggcgttaatg gcgtcaatgc ccgcctgcgc
    42841 atcttcaatg ccgatacatg cctgcggcgg cacgcccagt ccggcacagg cggcgagaaa
    42901 gatttccggg tccggtttcg agtttttaag ttgggaagcg tccgcgcaga aggtgaaaaa
    42961 ctcgcgcagc tccagcgctg ctaaaatcgt cggcgcattc agggagacag aagccagccc
    43021 tatagggatc tgctgtgcac ggagatctgc cagcaaattg cgaatgccgg gcagcacagc
    43081 attaaccgtc agttcgcgca gtgagtggac gtagagcaga tttttgcgat acgccagttg
    43141 agccctctcc tgcgggttaa agtcgccctc tttgcccccg tgttgcagaa tgcgccgcag
    43201 agactcatcg cggctgatcc cttttaggga ttcgttaaac tgcgcatcaa tgctgatgcc
    43261 aatttcagcg gcaatctgct gccacgcctt gaaatgcaga tgcgcggtat cggtgattac
    43321 accatccaga tcgaaaatta ccccttgcag tttcattcat cctcctgatg cgtggtagcg
    43381 gtcccattga agggcaaaat aaaatcaccc aaacagaaaa cagattcttc agccacagat
    43441 ataagctgcc cgttcaaacg cagtgaaacg ggcgcagaag ttcgaatcgc aatacgctgc
    43501 gcgtcgagag tgacctgtaa ttcgcagccc tgccagaaca gagggaaaga caactgttgc
    43561 cactgctcag gtaacgctgg attgagatgc aattcaccgt tacgcacgct caccccggca
    43621 aaaccctgaa tcgcccccag ccaaatagcg ccagttgcgg ctgcgtggat accgtcatca
    43681 cagctatgcg gatcagcacc aagatcgatt tcagtcccct cccgccagaa ctgataactt
    43741 tgcgccaaca ggccacagcg tgcggcaaca atgccgtgga tcgctttact cagtgaagag
    43801 tcgtgaatgg tgcgcggttc ataaaattgc agattggcaa gacacgatgc cgctgagtac
    43861 tgctccggca gcatgtaatt gagcatcacc acatcagctt gtttgaggat ctgcatctcg
    43921 ttcacttctg cgcgtgaata atccagcaga atggtttgct tccccgccgc cgctttgtat
    43981 ttcgccagat taatcgctgg tttcgccata aacgaatcat cctgcggcaa aacgccgtca
    44041 ggctgaattt ctggcagccg cagttctttg aggaatattt cggcacgatg aataaacgcg
    44101 tcgtcgctac aaccaaactg acgagcgata ttcagcgcct gctggacgtt gtaatacgcc
    44161 atatagctgg tgaaggcgtt attattgaca tgttcggtat attcgtctgg cccaataaca
    44221 tcatgaattt ccagacggtc gttaaccctc accgtgcggc taatccagaa ctttgcagtt
    44281 tccagaagta gcgccatgcc ttcatgagca atgaaacttt catcccccgt ggtctgccag
    44341 tattgaacaa ccgcccaggc gatatcggcg accagatgat gttccgcctg cgccgaggcc
    44401 actttttgcc gcagcccggt gcgaatgtta atggcggcaa attccggcgt ctcttcttcg
    44461 ccgctgcgcg cgctttccca cggaaatagc gcgccctgcc agccgttgcg tcgcgctttc
    44521 tcctgcgcgc ctggcaagtt gtgccagcga taacgcagta aacttcgggc aaccgtcgga
    44581 tcgctaaaca gatgaaacgg cagtaaaaat acttccgtat cccagaaaac gtggcctttg
    44641 tagccttccc cggtcaaacc ttttgccgca atactgctgc gctcgtcgtg agcaggcgtc
    44701 atgatgcgta ggtgataaag cgcataatct aacgcttgct gatcgtgcgc atcgccaccg
    44761 tttaccgtga tacgacgttt ctgccaccat tgacgccagt tttctgtgga tgctgcaaga
    44821 agttggtcgt aactctgctg tacatacatt tcaagctgac gaagcgatgc actgccccac
    44881 tcgtctaaag cgacgagtct gtcgtcccgc cagtcgatcc acacccgttt ttgcagcgtc
    44941 agcgtttcgc ctgcgtgaag ttgcgcactg gtatgttgca gcaagcggcg ctctttggcg
    45001 gtatagcatt gctgcacatc accgctcacc ttacagcaac agctgatggc cacatcactg
    45061 cgcccatcct gggtggtgta gatcccctgc atcagatgct gaccaaatac ccgcaactgg
    45121 gtttcgtcga gatgttggcg accgtggttg gtttgcgtgg cgtcaatgcc tgttgaaatc
    45181 agcactaacg cgtcggcgtc cagtggcgta atagtgattt ccagcgcaat gagcggcagt
    45241 tggtctgccg aaacaaagcg acggctgacg atggtgtaac gcgcgccgtt actggtacac
    45301 cagacaacgt tgcggcgtaa ttcgccactg gcaaagtcaa gctcacgctg ccaggcttcg
    45361 cgggataacg agaaaacctc accatttatg gcaatctcca tccccacgac atcaggcagg
    45421 ttcaccagtt cgttgatttc acccttaccc gctcgatgat acagccccgc cagatacatg
    45481 ccgcgcgtct ggcgcgtgta atcttcttca tggctggcgc gaagcccaag atagccgttc
    45541 ccctgcgcca tcagtgaagc atacttgttc agggtatgct ggctgaaatg gggttctgat
    45601 aacgttactg gcctggtcat aattcaacac gcgtccctgt ttctgcggat tgatacaacg
    45661 ccgccaccag ttgctggatg atgtacccct gctcggcgtc ggctatcatc acgggcttgc
    45721 cctgtacgtg attgataaag gcttccatgc tgcgcaaatg gcggttgtcg tctgccattt
    45781 cccgttgcat cagcgtcatt aattcaccgt tgttatcggt gtagagatgt gccggaaaca
    45841 gcgtcgcacc ggctttatca ccacaaaagc tgacattcat aatcgactgt tcgcggatgt
    45901 tgagtgcaaa tgacgtttcc agccacagaa tgccgccgtt atgaaattca atggtgccaa
    45961 acagcgaatc ttcgacgctg taagttgccg gatcccactc gccaaattga ccacagctct
    46021 tttgtgtgcc gatcttttga aagctatgcg cggtcacgct tttcaccgcc ggaaagccca
    46081 gtacatacat cgccgcatcc agcatatgaa tgccgatgtc aataagcggc ccgccgccct
    46141 gcaattcttt attggtaaaa acgccccagc cgggaacgcc gcagcgacgc agggcgcggg
    46201 cggtggtaac gtaaatctct cccaaaacgc cgttggtcac ctgttcacgc agctgttgcg
    46261 tatcgagtgc aaaacgatgg tgaaagtcgt aggccagcac cttgccctgt ttgcgcgcgg
    46321 tatcgcacat ttcccgcgcc tgttctggcg tcatggcggg cggtttttcg cacatcacat
    46381 ggcagcccgc ttccagcgcc atcagggtat gttcgtaatg aaaacggtta ggtgagcaga
    46441 cgctaaccac atcaggtttc accgccagca gcatggcctg cggatcgtcc cacacggatg
    46501 cattcccgta tttttctgcc agcgcctgcg cctgggaaag gcggctgtca cagacagcca
    46561 ccagttccag atcgttgcgg gtgcagtagt acgaagcatg aactttatcc gccacctggc
    46621 ccgcgcctat tatcgcgacc cgcagcggag agcttgtcat tgcacttttc acaaagacct
    46681 cttagcaggt acgcaaccag gccaaagaat cacggtacgc ctgggctgga tcttccgccc
    46741 ggatgcgccc ttcgtacacc acatagccct gatagttatc cgcgcgcagc tgttcaaaca
    46801 gcgcgtggaa atccagggtg ccgctgcccg gctggtagcg atggttatcg gcaatatgca
    46861 catgaccgag caggtcgcgg ttgtcatgca gcgcctgcgc caggttatct tcttcgatat
    46921 tcatgtgata gaaatcgccg ataatctgta catgtttaag atcgttttcg acgatgtaac
    46981 ggcgggcatc ggcgagggtg ttgatcatgt gatcctgata acggtttaac ggttcgaggt
    47041 acaccacggt tccggtacat gcggccacct tttccagtac gcgcagggaa tcactcacca
    47101 ttttgcggtc gccgtccagg ctacgcggcg aggtcatcgg cggtaagcgg aaggtaaaca
    47161 tgccccatgc agccggaacg acgatacctt taccgcccac ttctgccaga gcttcaagaa
    47221 tgcgttcgat ctgctttaag ccattaagtc gacgctcttc gataaagtcg ccaatccaac
    47281 cgtcatagcc accgcaggcg gtggtcaccg gtaaaccggt ttctttgatt gctgctttga
    47341 cttcttcgag attgttgacc agcagtttgc cgtcaatctc aaaaccatcg aaacccatct
    47401 ctttgatata acgaaatttc tccagaatgt tttccggaaa gaacgcctga ttctgtgtgc
    47461 cgattttcat ttttttcatt cctttgaatt aaaacgtaac gcccattttg atgctctgtt
    47521 ccggatgctg gtcgacatac tgcatgtagc tttccgggct gttagcaaag gtcaccaccg
    47581 ggtcgattaa atcttcacaa ttgagataac cgttcatcag cagttcccag caggtttctt
    47641 caatacgctt gcgactccag cgcggataat ccgggttcgg ctcgctgcac gcacgggaga
    47701 aaacaatttt ggcgttgttg aaatgcgctt cgcgtccgag gttaaaacct tcggcaaacg
    47761 gtttggcaaa cgcgacatac gagatggtgc cgccatatgc cagacctcgt agcgccgatt
    47821 gcagcgcgtc ggcatagccg ctggtttcga taatcacatc ggcaccctgc ttgccggtta
    47881 gcgttttgat ctctttgcct acgtcagtgc caatggggtt aaggcagaaa tccgcgccat
    47941 ggcgacgggc aatctcacag cgatgggcga taggatcgac gccaatcacc accgacgcgc
    48001 cagcacgttt agccagttga atggcaattt gaccgatcgc gccaagccct accaccacca
    48061 caaaatcgcc cacgcgcaca ttggcatcgc gcacgccgct catggcaaac tgcgccgggt
    48121 cgtagcagac agcgtttttc cacgaactgc cttgcggcat tttgcgcagc ttgtagttat
    48181 tcactgcgtt aatgatgacc gtctcggaga gcgggccgta gccgcatacg ctgtcgccca
    48241 ccgcgtagtc ggtaacgtcg ctgccgcact cgataatgtc gccaaccacc atgttgccaa
    48301 gctggaattt gccaaactca atgccgcgcg gcgcatctgc cggacgcggg gtgaacatct
    48361 gccattcgcc gttaaagtct tcatcgataa acggactggc agcgcggaaa tcgaccactt
    48421 ccgtcccgtg cttcggtgcg ccgaagcgca cgcggatctt cacttcattg gctaaaatcg
    48481 cccgatcttc atactcaacc agcgcagcaa cgcgcggtgc tgtggctact aactttttca
    48541 tgtaaaactc cttgtaaaga aactcgccgc tttagccctt cacgccaccg gcggtcaaac
    48601 cactcttaat aaaacgttcc gaaagcgcgt acataatcac caccggcaat gcggtcacca
    48661 gcgaggccgc catcatccgt ccccagatgt aatcaggcgt actgaacagc gcgttcagtc
    48721 ccacaggtaa ggtgaaattg ctggcgctgg agaggaaaat cgacgcaaac agatagtcgt
    48781 tccacgccac cataaagcag tagacgaaca cggatatcag cccggacatc gccaggggca
    48841 cggtgatacg gaagatgatt tgcagacggt tgaggccatc catcatcgcc gcttcttcga
    48901 tctcatcagg gatggtgtcg aagtagcttt tcagcataaa cacggcggtg ggcagggttt
    48961 gcgtcaccat ggtaatgatc agcgccatct cggtgtcgta aataccaagc gcggtgatga
    49021 ttttgaacag cggcaccacc agcaaaatgc cagagaacat gtacaccgtg taaaagctgg
    49081 cgttgatggt catccggcct ttaaagcgca ggcgggaaag cgcgtaagct ccgagaatgc
    49141 cgagaaatac cgccaccacg gatgaaacca ccgacaccac catgctgtta cggaagtagt
    49201 cgacaaacgg gaaaatcatc gggttaaaaa tgtcgacata atgctccagc gtccactgtt
    49261 gcggcagcag cgtaggatgc agcgagatcg cctcttttgc gctcttgaac gaggtcatca
    49321 gcatcacaaa aaacggaaac agcgtgatga tgagaaacag cgccagcccg cagtaaaaac
    49381 cgatgcgact gagtgtgcgt ttatttgttg ccattgaggt tcacccgttt tctggtcagc
    49441 agaatgacag cgaaaataat gatgaagagc accaccgaga tcgccgccgc tttccctaag
    49501 tcattaaagg caaaggcggt tttgtagaga tagacaccga gaatatcgac cttggtcgtc
    49561 agcagataaa catccgcgaa catgtagaac atccagatgg tgcgcagggt caccaccgtc
    49621 gccaggaccg gcataatcgc gggcagcgtg acgatacgaa accgttgcca ggcattagcg
    49681 ccatccatct ctgccgcttc gtacagcgat ttgtcgatgg tctgcaaaat cgccagaaac
    49741 gaaataaagg catacgggaa gtagcgccag atggcgaaca gcaccaccag cacaaagcta
    49801 ctgcccggat tatcgaacca cagcggtgcc tgctcataga gatgcagaag atcgacgccg
    49861 aggtagttca caatgccgta accgttgttg aacatgtatt tccaggcgaa caccagcgaa
    49921 atagacggcg ttacgtagga gaggatcacc agcgaacgcg cggttttgcg caggcggaac
    49981 tcgcggttga aaaacatcgc cacggacagc ccgagcacgg cgctgcccgc caccaccagt
    50041 gtggtatacc agaccgtcat ccacagcgag tgccagaagc cgggatcgga gagaatacgc
    50101 acgtagttgc tcaccccaac aaacgttgac tcgatgttgg gattaagcgg cagacgtaaa
    50161 aaactgattt cgatattcga caccatcggc cacgccacca gaccgcccag cagtaataag
    50221 ctgggcgcga gaagcaacag cgcaaagggc atatcggaac gacctgaaaa aagcctgttc
    50281 attgcggatt cctgtcctgg gtttagcgct gttcaaccaa ctcatccagc tttttctggc
    50341 tcgtttgcag cgtgccaggg aggtcggctt tacccacggt gacgttatgc accatggagc
    50401 tcaccacgcc agaacccgtc acatcgccca tgcgggtaaa atttttatcc cctactgcgc
    50461 caaagacctg aatatttggc agttcgctga ttaactgatt tggcagctca cccagcgcct
    50521 taataacgtc gttgtctttc caggtggcgg tagtgacaac cgctttgttc accggcagcg
    50581 ccgcgcctgg cgacatcatc acccagtcgg caatgttgtc tgcctgctcc ataaaggtga
    50641 caaatttttc agccgcttcc gtctcttcgg ccttttgccc ggcggtaatg gtcagcgagg
    50701 tcaacatgcc gtagaccgca gagtttttct cggtgggtac aacgaaacca acgtttttcg
    50761 gatcgccctc tttaatcaca gccggaagga tataggtgga gtaaatcgcc atcggcgcgg
    50821 tgccgttcat aaaagcgtct ttcacttcca tgatgtcgtt agaacccggc atggtgttgg
    50881 cagcaaggtc gcggtaatag gtcagcgcct gcatcatctc tggggtatca agggtgattt
    50941 tgccttcggc gttaaagacg ttagcctgat tggataacgc aaactgggag aaagattgtt
    51001 ccgtcagcac actttctgcc gtaggcagtg caatgccata ttttttattc gccgggtcat
    51061 tcagtttctg cgcgacatcc agcaattgct gccagttttt cggctcctcc agcccggctt
    51121 ttgccagcac atctttgcga taccagatgc cgccaatcca tgcgctcacg ggaacaccgg
    51181 tccaggcgct gccatcttcg gtgcgcacaa tacgcagtac gccatcgtag aacgcgcctt
    51241 caccaacgtt gctgatgacc gtggcaaccg ctttacgatc gataagctgc tctttgtcca
    51301 taactttggc gtagtcatgg ctggtttcga tcacttccgg cagcgagccg ctacgtgaca
    51361 gggttatgac tttggtgtta taggcatctt cttccactgg cacttgttta acggtgatgc
    51421 ccgggttttc tttttcaaaa cgggcaatca atttactgat aacggcctgg cgctcctgct
    51481 ccaccgaaga atgcataaat tcgatggata catgggtttt attttcttct ttacagcctg
    51541 aaatcagggc gcaggaaacc agtgctgata acagcacgat ttttgattta atcatgagat
    51601 gttccttttt atttaatcca cataacctgc caggggcgca gcgttatttc attacccaat
    51661 atatcaactt cactaatcag atcccgacca tgcagatcag taatatttat atgctgaata
    51721 ttttcactga cattaaacaa tcctgtcaga caattaccat ccgcgttgct tctttgaata
    51781 cacattacgg atgaattaac cgtatcgatg gtaaaatcat tatccggatg aaactcgtta
    51841 tggctgcgac gaagtgtaat taaacgcgac aactgatgat ataccgcatg ccttaatgta
    51901 gcttcatcgt tcagttcctg tgttatctct tcactgtgat attttttacg gttaatcgca
    51961 cggttatatc cgagtttttc gacacctgcg taatcattac gcgagcccag aatactttga
    52021 atatatatcg ctggcacacc ggggaaactt aacaaaatcg catgggcaag gataaacctg
    52081 gcacaacgtt ctacatcgct actctcgcgg cggcttaacg catccatata ggtgacattt
    52141 atttcatagg gactgcgcgt accgtcggga ttatttttcc agtttactaa tgcaccttcc
    52201 tgctgtaatg cctcgaccag cgctaatatt tcgctttctg gtaacaagcc acgcagtggg
    52261 ttcaacccga tgccatcgtg agaggcgagg aagttaaacc aggtggtgtt gctggaaggt
    52321 agtgtcaggt tttgcgccca cgcacaaagc gcctcaacgt tctgtttttg caccgcatgc
    52381 agcaccagcg gcggcagcga gaactggtac accatatgcg cttcgtcatc gccttcgcca
    52441 aagtaagcaa tgttgtcttt atgcggaaca ttggtctcgg taatgatcac tgtacctggt
    52501 gctacgtcat caataatcga ccataacagc ttgataatca gatgtgtttt ttccagatgg
    52561 atgcagcttg ttcctggctc tttccacata aagccaacgg catccagccg gacatattct
    52621 gcgccctttt ccaggtagca cagcaataca tccaccatcg ccagcaacac ttcagggcta
    52681 cggtaattga ggtcaatttg atcgtcgcta aaggtggtcc ataaatggcg cgtggaatta
    52741 tcgcgcatct ggaatggcgt taataacggt aacgcacgcg ggcgagtgac ggcgctgaga
    52801 tcggtttgcg ggtcaacggc aataaaaaaa tcttcaaaac ctggctgctg ttgtaaatag
    52861 tttttaaacc attcactttt tgccgacata tggttgcaga caaaatcaaa cattaaatga
    52921 ctgcattcac cgagttgctg aatatcctgc cactcccccg cttcactggc gacctgatga
    52981 taatcaatta ccgaaaagcc atcatcagat gaccatggat aaaacggcaa caaatgaaca
    53041 tgtgaaaaaa tgctttgaag ccattgaggg taaaactgat taaatgtggg tagtggtttt
    53101 aaatcattac tgtgaaattg atcagcatag gtaattaaca cgacatcact ttcatcccag
    53161 tgttttttac gtcgctgtgt aattaatcgt ttcgcactct caagacgcgt tacaagtttc
    53221 tgtaaatgag ttgcggtaaa tgttccaccg tagatttcgt ccaggtaatc cgtaattttc
    53281 tgttccatgt tgcctttccg tggtagcggt cccatgaaac gagagactac tccgcagaaa
    53341 ataaactgtc aattgagcac aagggtttta gtcacgcagg aaatcatttt ttaccaggga
    53401 aaaagcgtat ttgtgagctg acgcaaataa tctatgggga tgtacataag gtgtgacaga
    53461 aaaagtaaac ggcgcataag cgccgcttag ggtggattct tttaaccttt gaccttcggc
    53521 attgcgatgt ctttcaggcc accggcgttc tcaacgtggg tatatcccat ctcgctaagg
    53581 atctcttttg cctgccctga ctggcgtccg gcattgcaat acactttcac ggtgtcgttt
    53641 ttatccggaa cggcggtggc aatgcgctct ttcacttcct tcaggggaat attgatggcc
    53701 ccctgaacgt gctcttgctg atactgctca ggaacacgaa catcgatcca gtgttcagcg
    53761 gcgaaaacgg gcagtgaaaa caccagtgtc agagcaagta agcctttttt aaacatgtaa
    53821 gcgtccttct atggatgaaa attgccatcc ataatacgcc ttagctttaa caatttttgc
    53881 gaaactcccc tcacctttta taacgctgcg ccagtttatt ggcagcccga cttaacagcg
    53941 gttccagcgc cacagccagc aacattttca gcggtcggcg agcaactgat tttatcgccc
    54001 agcccgccac acccgccggg ccatagcgca gtgcggtaag aagtaccagc ttgcctgcta
    54061 atttgaaacc aggcttcacc ttttgcccgg cctgttgcca gcgagtattc ataactttcc
    54121 tcacagttga cggaaacggc tacgtaacgt gaaagtatcg gaagtgacat aacgttccat
    54181 ctcgcgtaaa cgcgtttcac ttgccgccag ttcacggtcg acttcatcca gcaattcact
    54241 gctggaaggt agctgctcac caaaggccat gttgtccggc attggatcaa gcgcaaatga
    54301 caaaatgatg taagcaacca gggtaaacag cgccagaccg aagaaaatcg acagcaccac
    54361 caggatacgc accagtttta ctggtacatc aaaatagttg gcaatcccgg cgcagacgcc
    54421 acggaccatg ccctgctgtg gaatacgcca taatttttta ttgagattaa tgcccgccat
    54481 tagcgatccc tccagttcgg atgttctgca tcaagaatgg attccagcgc ctgaatacgt
    54541 tcgcgcatcc gttttgcttc atcagccagt tgcgctaatc gctgctgctc actttgcgac
    54601 aattcactgc gaccagaacg attgctgtaa tgcagccata accagatcgg taaaacaaac
    54661 agcacaaaaa tggttaacgg aatagccaga aatagcgcgc tcataaggac tccttagacg
    54721 atgagcggcg gcgcgttcgg acgccgcctg gatgttatta ttgattgtct tgcttcattt
    54781 tggcttttaa ttgtgccagt tgctcgctga ttgcatcatc ggctttcagt tcggcaaact
    54841 gatcgtccag cgatttttgt ttaccgaagc tgtggctttc tgcttccgct tccatctggt
    54901 caattcgacg ttcgaaagat tcgaaacgag ccattgcttc atccagtttg ccactgtcca
    54961 gctgacgacg cacatcgcgc gacgagtttg ccgcctgatg gcgtaacatc aatgcctgct
    55021 ggcgagcgcg tgtttcgctc aatttgtttt ccagctcgcc gatctctttc ttcatgcgtg
    55081 ccagcgtatc gtccaccagc gtcacttcat gttccaggct cttaatcaga tcggtcagtt
    55141 tctgtttttc aattaacgcc gcacgcgcca ggtcttctct atctttcagc agtgccagtt
    55201 cggctttttc ctgccattca acttcacgcg ccgacgcttg ttcaatacgg cgagtcagct
    55261 gtttcttttc tgccagcgca cgcgccgaag tagaacgtac ttcaaccagt gtatcttcca
    55321 tctcctggat catcagacgc accagtttct gtggatcttc cgctttctct aacagagcgt
    55381 tgatgttggc attcacgatg tcggcaaagc gagaaaaaat acccataatg ttgtcctctt
    55441 gatttctgcg ttcaggattg tcctgctgaa ctattaatac aatttgcgtg ccaatttttt
    55501 atctttttga tttataaaga tctgattgaa gaatcaacag caacatgcca ggatgaatta
    55561 gcgaattaca ctaacaagtg gcgaatttca tcatggcaga atacaaagat aatttacttg
    55621 gtgaggcgaa tagctttctc gaagtgctgg aacaggtttc gcatctcgca ccactggata
    55681 aaccggtgct catcatcggc gaacgcggca ccggtaaaga gttgattgcc agccgcctgc
    55741 attatctctc ctcccgttgg caagggccgt ttatttccct taactgcgcg gcgttaaatg
    55801 aaaatctgct ggattccgaa ctgtttggtc acgaagcggg ggcgtttacc ggtgcgcaaa
    55861 aacgtcatcc tgggagattt gaacgtgccg atggcggtac gctatttctt gatgaactcg
    55921 ctacggcacc gatgatggtg caggaaaaat tattgcgtgt gattgagtac ggtgaactgg
    55981 agcgcgttgg cggtagccag ccattgcagg tgaatgtgcg gttggtatgc gcgacgaatg
    56041 ccgatctccc ggcgatggtc aatgaaggca cttttcgcgc tgacctgctc gaccggctgg
    56101 cttttgatgt tgtgcaactg ccacccctgc gcgagcgcga aagcgacatt atgctgatgg
    56161 cagaacactt tgccatccag atgtgtcggg aaatcaagct gcctctgttc ccggggttta
    56221 cggagcgcgc cagagaaaca ttgctgaatt atcgctggcc gggaaatatt cgtgaattga
    56281 aaaacgtggt ggaacgttca gtatatcgcc acggcaccag cgattatccg cttgatgaca
    56341 ttattattga tccctttaaa cggcgtccgc ctgaagacgc tatcgccgtt tcagaaatca
    56401 cctcgcttcc aacactgccg ctggatttac gtgagtttca gatgcagcag gaaaaagagt
    56461 tgctgcaact cagtttgcaa caagggaaat ataaccagaa gcgcgcggct gaattactgg
    56521 ggttaaccta tcatcagttc cgcgcgttgt tgaaaaagca ccagatttag cgaagttggg
    56581 caatacaact tgccggatgc ggcgtgaacg ccttatccgg catacataat caatacgttg
    56641 tttgcaggcc tgataagcgc agcgcatcag acattattgc gttgggctat taatcgttca
    56701 gcgcatcctg caaaattttc attgccgcat cgaattgcgc atccgggatg gtcagcggat
    56761 acaggaaacg aatcacgttg ccgtatgcgc cacaggtcag cagaagcagc ccctgcgcca
    56821 gcgcgcgttg ctggattttc tgtgcaatcg ccgctgacgg ctcgcccgtt tgcggatcgt
    56881 taaactctgc cgcaatcata gaccccagac cgcgtaccgc cgcgatggcc ggaacgcttt
    56941 ctttggcatc aatcaacgtg tttgtcaggc gctggcccag ttgattcgcg cgttcacaga
    57001 gtgattcttt gtcgataatg ttgagtacag cgtgcgcggc agccaccgcc agcgggttac
    57061 cggcgtaggt gccgccaagc ccgcccggcg cgggtgcgtc cataatattc gcgttaccga
    57121 ccacgcccga aagcggcatc ccgcccgcga ggcttttcgc catcgtcatt aagtctggct
    57181 tgtcagcata atggtccatg gcaaacaatt taccggtacg cgcaaaaccg ctttgcactt
    57241 catcagcaat catcacaata ccgtgctcgt cgcacaggcg gcgaatggcg gcaaccagct
    57301 cttttggcgc aacgttgaaa ccgccttcgc cctgcaccgg ttcgaaaata atcgccgcca
    57361 cctgcttcgc ttcaatgtcc gatttaaaca agcgttcgat ggcgtcgagg gagtcctgcg
    57421 ttgaaacgtc atgtaaatct gacggataag gtacgtgata taccgaaccg gggaacgggc
    57481 cgaagccgat tttgtacggc gcgacctttc cggtcagcgc catggtcata tatgtacgac
    57541 cgtggaagcc gccgctaaac gcaatcacgc cagggcgtcc ggtatgggcg cgggcgattt
    57601 tcaccgcgtt ctccaccgct tccgcaccgg tggtgaagaa cgcagtttta gcctgcccgc
    57661 tcaccggggc aagggcgttg attttctccg ccagggtgac gtagctttcg tacggcacaa
    57721 tctgatacgc cgtgtgggta aactgttgca attgctgctc caccgccgcg accagatcag
    57781 ggtggcgatg cccggtattc agcaccgcaa tgcctgcggc gaaatcgatg tattcgttgc
    57841 cctctacgtc cttcagcatg gcgttttctg ccgactgggc gaagaagtta cacatcacgc
    57901 caaccccgcg aggggtagca gaaagacgac gctgatggaa ttcattgttg ctcatgtttc
    57961 acaccttttg actcttcact gttaatcgga aattaaacgc ccagacgatc gcgcaggctg
    58021 taataagccg cgcccatcgc ggtaaacgga acgcgcagcg tgcgcccgcc ggggaacggg
    58081 taatgcggca gattggcgaa ggcgtcgaaa cgctcggcgt caccgcgcag cagttcagca
    58141 atcaaacgcc cggcgaggtg ggtacaggtg acgccgtggc cgctgtagcc ctgcatgtag
    58201 tagatgttgg tatcgaggcg accaaactgc ggcatgcgtg acagggtcag caggaagttg
    58261 cccgtccagc ggtaatcaat tttcacgccc ttcagttgcg ggaaggtttt cagcagtttc
    58321 ggcaccacaa ggcgctcaac atcgtccggg tcgcgtgcgc cgtagaccac gccgccgccg
    58381 tacagcaggc ggttatcggc ggtaagacgg tagtaatcaa gcagatagtt acagtcttcc
    58441 acacagtagt ttttcgggat cagcgaacgg gctaaatctt ccgacagccg ttcggtggtg
    58501 atcacctgcg tgccgcacgg catgctgcgt ttcgctagtt ccggctctac tttatcgccc
    58561 aggtaggcat tcccggcgac aatcacatac ttcgccgtca cctgaccatt ggcagtccgc
    58621 accacggctg gcgtggtgtg ctggatctgc gtcactgcgg aaagctcatg cacgcgcccg
    58681 ccgttcaggc ggatggcatc ggcttcacca atggcgaggt ttagcgggtg aatatgccca
    58741 ccgctgtgat ccagcagcgc accagtgtag cgatcgctgg cgacctcgcg gcgaatagcg
    58801 ttggcgtcca gcagctccag ctgtttattg ccgtagcgtt cccagttctc tttctgctct
    58861 tcgagtgtgg cgagctgttt atcattcatc gccacaaaca gaccgccggg gcgatagtcg
    58921 cagtcaatct gataacgttt gatacgttcg cggatgatct cgccaccctc gaacatcatg
    58981 ctgccgagca tacgggcggt atccatgccg tagctttttt cgatcacgtc gatgtcgcgg
    59041 ctgtaggagt tcacaagctg cccgccgttg cgtccgctcg cgccaaagcc gatgcgtgag
    59101 gcttcgagaa ccaccacgtc aaagcccgct tccgccagat gcagcgcgga ggaaagcccg
    59161 gtatagccgc cgccgaccac gcaaacgtcg caggaaatcg actcattcag cgtgtcgaat
    59221 ggcgcgtatt tattcgcact ggcggcgtag taactgctgg tgtgttcggt catttcaggc
    59281 ctccaggctt atccagatgg ttttcagttc ggtgaatttt tcaagggcat gcagggattt
    59341 gtcgcgcccg ttgccgctct gcttatagcc gccaaacggc acggtcatat cgccgtcgtt
    59401 gtagttattg acgaagacgg agccggcttt caggcggcga ctcatgcggt gcgcgcggga
    59461 gaggtcgcgc gtccataccg ccgcgccaag tccgtactgg ctgtcgttgg caagctgtag
    59521 cgcctgttct tctgatgtga aacgcgtgac caccagcacc ggaccgaaaa tctcttcgcg
    59581 gcttaaggac gcattcgggt ctacctccac aaagatggtc gggccgatgg cggcagccag
    59641 ctcggcgtta cggccatcca gcagcagttg ccctttgctt tcgccttccc gaataaagct
    59701 atggaccgag tcggcgtggg cgcagtcgat taaggtgccc atggtggttg cgggatcaag
    59761 cggatggccc ggctgccagt tttgcgcttg ctgttttaac agggctaaga attcatcggc
    59821 gatgctctct tccagcaaca ggcgcgttcc ggcgatacac acctgcccct ggttgtagaa
    59881 aatgcccgca gcggtcgcgc tcgccgcttt ttgcaaatcc ggacagtcgg cgaaaacgat
    59941 gttggcgctt ttgccgcccg cttccagcca gacgcgtttc atgttgctgt cgcccgcgtc
    60001 cttcagcagc tgtttcccgg tgcgggttga gccagtaaag gcaatggcat cgatatcgtt
    60061 atggcgcgaa agcgcctgcc cggcttcatg accaaaaccc gtcaccacgt tcaacacacc
    60121 atccggcagc cccgcttctt tcgccagccc cgcgagacga atcgcactga gcggtgattt
    60181 ttcagacggt tttagaatca cgctgtttcc cgccgccagc gccgggccga gtttccagca
    60241 agtcagcagc agcgggaagt tccacggcac gatggcggca atcacgccga ccggttcacg
    60301 cacgatcatc gccagctcat ggctactggt ggtcgccact tcgccataca ctttgtcgat
    60361 cgcttcggcg taccagcgaa tggcgcgcgc cgcgccggga atatcatcac gcaggctgtg
    60421 acgaatcggt ttaccggtgt cgagcgtttc cagcagcgcc agttcttcag cgttggcttc
    60481 cattaaatcg gcgagtttat tcagcaccgc tttacgctta gccggagaag agagtgacca
    60541 gtcgccacgt tcaaacacgc cgcgtgctgc gctcaccgca cggtcaatat cgacgctctt
    60601 gccgcgggca atattcgcca gcggtgcctg ggtgaccgga tcaacggttt caaaagtttc
    60661 attttccgcc gcagcagtat attcaccgtt aataaataag cgggtttcaa tggcgagact
    60721 taacgcttta tcctgccagt aagccagatg atgaaaattc attatgactc ctgtttcacg
    60781 tctatcagat atatgcagtt tgtgaacgtc agggcgcaga gatccccggg ccgtatgccc
    60841 gtttatttct ggcgacattc tttatagatt gccgggcgca ttgcgcttac ccggcttaca
    60901 gaacaaaaag attaaaacgt ggtgggcgta tgggcgctga taattcggca aatacctgcc
    60961 gacgtattac tgaaactgtg cgggatgccg gtattaatgg cataactttg ccccgcgacg
    61021 agatgataat cctgaccatt aatcgtcaga acaatttcac cttccagtac agtgcctatt
    61081 tcctcaccct gatgcttaat tctttcccca gtggttgtgc ccggctggta agtttcaaag
    61141 atcatcgcca gcgtgcgatt cgggttaccg ttatgaacca gcttcattga cacaccctga
    61201 ctgcccatct caattaagtc gtcctgatta atgacgacct gcggctcatc aggtttttcc
    61261 ggctcggaaa agaattccga gagtgacaga ccatacacct tcagcagctt ttgcagcgta
    61321 ctgatggcag ggctgacttt atcttgttct atcgtactga tagcactgtg agtcagcccg
    61381 gagagttcgg cggcacgacg ttgtgaaagc ccctgctgct ggcggatttc cgacaagcgt
    61441 tttcctggcg ccagtccctc atcactcata tttgcatttc cttaaactgt agtggtcaga
    61501 gtcgctgttt ttcagcgata tggtgctgac aagcggtgat aaagccctcg aacaatatac
    61561 gcgaaagcgc gtactcgcta ctgttccatt ccgggtgcca ctgtacgccc agcgcaaaag
    61621 gatgattgat gacgctaacc gcctcaacta agccatccgg cgagcgagct tcaacacgca
    61681 accgtgggct aacgaccttc gccccttgtc catgcagaga gtttacccaa aagttgctac
    61741 attcaggtaa caacgcagac agtaatcccc cctcttcaac ctgaacttca tgcgacggcg
    61801 catattgctg ctccaccggc agttcgggat cttcccgatg ttccagcaat tcaggctgtt
    61861 cgcacagctt gcgatgcaac gaaccaccgg ttgccaccac cagttcctgt aaaccccggc
    61921 agatggcgaa aatggggatg cgcctttcga gtgcggcatt aattaacgcc atgctcagaa
    61981 gatcacgccc aggatcggcg tcaggctcat cgccgttttc accatatagg tgcggctgca
    62041 cattgctggg actaccagga agataaatgc catcgagttt cggcaaaagt tgttcaagta
    62101 atgacggttc cgccagcgca tgtggcagcg caattggcaa gccgcctgcg tggatgatgg
    62161 cattcaggta cttttcttgc agagtctggg tcgcatgacc cttaagcctg ttcctgcaca
    62221 ttacgacacc gataaccgga ttgttcatta tattttccat gctcaatctc acaaagtgga
    62281 ctaaattatc gccattactg cgctttaaat gacctttatg ttcaatattt tttcaatcta
    62341 gcagtgggtt ttcatttttg caaactcaat ttaacatttg acaaacattt agtttgcata
    62401 cagattcgaa tagtggtcat tatattttac gctttgataa cgagcggaaa acaaaccaaa
    62461 ggcgaagaat catggaaacc aatatcgttg aagtagagaa ctttgttcag cagtcagaag
    62521 agaggcgggg tagcgccttt acgcaggaag tgaaacgcta cctggagcgc tacccgaata
    62581 cgcaatatgt tgatgtttta cttaccgatt tgaatggttg cttccgtgga aaacgtattc
    62641 ctgtttcaag cctgaagaag ctcgagaaag ggtgttattt cccggcctcg gtgtttgcaa
    62701 tggatattct gggcaacgta gtagaagaag cgggcctggg tcaggaaatg ggcgagccgg
    62761 atcgtacctg tgttcctgtt ctcggttcct taaccccttc tgccgccgat ccagagttta
    62821 tcggtcagat gctcctgacc atggtcgatg aagatggcgc tccctttgac gttgagccgc
    62881 ggaacgttct taaccgcctg tggcagcagc tgcgccagcg cggattgttc ccggtcgtag
    62941 cggtagagct ggagttctat ttactggatc gccagcgcga cgctgaagga tatctgcaac
    63001 cgccctgcgc gcccggcacc gatgaccgca atacacaaag ccaggtttac tccgttgata
    63061 acctcaacca cttcgccgac gtgctcaatg atattgatga gctggcgcag ttacagctga
    63121 ttccggcaga tggcgcggtc gccgaggcct cgccaggtca gtttgaaatc aatctttacc
    63181 ataccgataa cgtgctggaa gcttgcgatg atgcgctggc actaaaacgc ctggtgcgtc
    63241 tgatggcaga aaagcataag atgcacgcca cttttatggc gaagccgtat gaagagcacg
    63301 cgggcagcgg gatgcatatc catatcagta tgcaaaataa tcgtggcgag aacgtgcttt
    63361 ctgacgcgga aggcgaagat tcgccgctgc tgaaaaagat gctcgccggg atgattgacc
    63421 tgatgccgtc atcaatggcg ttgctggcac caaacgtgaa ctcgtatcgc cgcttccagc
    63481 cggggatgta tgtaccgacg caggcgtcgt ggggccataa caaccgcacc gtcgccctgc
    63541 gtattccctg cggcgatcgc cataatcacc gcgtggaata tcgcgtggcg ggtgccgatg
    63601 ccaacccgta tctggtgatg gcggcgattt ttgccggtat tttgcatggc cttgataacg
    63661 aactgccatt acaggaagaa gtcgaaggta acgggctgga acaggaaggc ttacccttcc
    63721 cgattcgcca gagcgatgcc ctgggtgagt ttatcgagaa tgatcacctg cgccgctatt
    63781 taggcgaacg cttctgccat gtctatcacg cctgtaaaaa tgatgagctg ttgcagtttg
    63841 agcgcctcat cacagaaaca gaaattgagt ggatgttgaa aaacgcgtaa taccccttgc
    63901 ggggttgttt catcggcctg agcggagcca ggcctgacat ttgctggtaa tccagacatt
    63961 tcccggatgt cgcgttatgg agcgcaggcg gcaacgggct tccgttaacg attaagactg
    64021 tgacgaggaa atgagatgat gccgatgatt aaatccccgc atggcgaggg aggctgcgta
    64081 tgcgcccctc ctgcgactga ctggactccg ccccctctgc ttcccttgct aaacaggttt
    64141 gatttcaggt cgacacgacc gcaaacctta ttacgcaggg gaggcagcaa ttatggctat
    64201 taattcacca ctgaatattg ctgcgcaacc tggcaaaacc cgtctgcgaa aatcactgaa
    64261 attgtggcag gtggtgatga tgggtctggc ctatctcacg ccgatgaccg tatttgatac
    64321 ttttggtatt gtctccggca ttagcgacgg tcacgttccg gcgtcctatt tgctggcgct
    64381 ggcgggcgtg ctgtttaccg ctatcagcta cggcaaactg gttcgccagt ttccggaggc
    64441 cggttcggcc tatacctacg cgcaaaagtc gattaacccg cacgtcggat ttatggtcgg
    64501 ctggtcatcg ctgctggatt atctcttttt gccgatgatc aacgttttgt tggcgaaaat
    64561 ctatctctcc gccctcttcc cggaagtgcc gccgtgggtg tgggtggtaa ccttcgttgc
    64621 cattttaacc gccgcgaatc tgaagagcgt caacctggtc gctaacttca ataccttgtt
    64681 tgtactggtg caaatctcca tcatggtggt gtttatcttc ctggttgttc agggactgca
    64741 taaaggagaa ggcgttggca ccgtctggtc acttcagccg tttatcagcg agaacgcgca
    64801 cctgatccca attattaccg gggcgacgat tgtctgtttc tccttcctcg gtttcgatgc
    64861 ggtgaccacg ctttcggaag agacgccgga cgccgcacgc gtgatcccga aagccatctt
    64921 cctgacggcg gtatacggcg gcgttatctt tatcgctgcg tcgttcttta tgcagctgtt
    64981 ctttcccgat atcagccgct ttaaagaccc ggacgccgca ctgcctgaaa ttgcgctata
    65041 cgtcggcggc aagctcttcc agtcgatttt cctctgtacc acgtttgtga acacgttagc
    65101 gtctggcctg gcgtcgcacg ccagcgtgtc gcgtctgctg tatgtgatgg ggcgtgacaa
    65161 tgtgtttccg gagcgcgtgt ttggctatgt gcacccgaaa tggcggactc cggcactgaa
    65221 cgtcattatg gtcgggattg tcgcgttgtc agcgctgttc tttgatttag tcaccgcgac
    65281 agcattgatt aacttcggtg ctctggtggc atttaccttc gtaaatctgt cggtgtttaa
    65341 tcatttctgg cggcgtaaag gaatgaataa aagctggaag gatcacttcc actatttgct
    65401 gatgccgctg gttggcgcgc tgacggtggg tgtgttgtgg gttaacctcg agtcaacgtc
    65461 actgacactc ggtctggtat gggcttcgtt aggcggcgca tatttgtggt atctgatccg
    65521 ccgctatcgc aaagtgccgt tgtatgatgg tgacagaacg ccggtgagcg aaacgtaata
    65581 gtcggatgcg cggcgcatta tcctgcctga gggtaatgca ccgcgccatt aactataaaa
    65641 agatgtatga cccggatcac gagtgctatc atccgccctt atgcttagcg cattaacccc
    65701 aggggcaaat aatgaaccac gatatcccgc taaaatattt tgatattgcc gatgagtacg
    65761 cgaccgaatg cgcagagcct gtcgcagacg ctgaacgcac accgctggcc ctctacttcc
    65821 agttactgct cacccgtttg atgaacaatg aagagatcag cgaagaagcc caacatgaaa
    65881 tggctgctga agcggggatt aatcccgtac gcattgatga gatcgcagag tttttgaatc
    65941 aatggggtaa tgagtaatta tttcattgca gactctcggt cagtatcgtt gtccggataa
    66001 agcgttcacg ccgcatccgg ctaacaatga cacgcactgc agcgtgcctc aatgggtttc
    66061 tcacgatcgt accgctctgc tcctcgctaa gttgttctgt gagttatagt tatagagaaa
    66121 tcacgggtac gaatgttgaa cagatggcga cgtcatctgt tcaacgacca accacacggc
    66181 cctcattacg acccctgaac acaaacggta atggcaaatg tcattaaata ttagtaagat
    66241 agcctcttta cataaaatcc attaatatta tgcgccaggt attatcgtct cttttggtga
    66301 ttgctggact tgtgagtggt caggcaatcg ccgcgcctga atcccccccg catgctgata
    66361 tccgcgacag cggttttgtc tattgcgtca gcgggcaagt caacaccttt aacccatcca
    66421 aagcgagcag tgggttaatt gtcgataccc ttgccgccca gttttatgat cgactgctgg
    66481 atgtcgatcc ctatacctat cgcctgatgc cggaacttgc cgaaagctgg gaagtactcg
    66541 acaacggcgc gacctatcgc ttccacctgc gtcgcgatgt tccgtttcaa aaaaccgact
    66601 ggtttactcc cactcgtaaa atgaatgccg acgatgtggt gtttaccttc cagcgaattt
    66661 ttgaccgcaa caacccgtgg cataacgtca acggcagcaa cttcccctac ttcgacagcc
    66721 tgcaatttgc cgataacgtg aaaagcgtcc gtaaactgga taatcatacc gttgagttcc
    66781 gtctggctca gccggatgct tcttttttgt ggcacctcgc aacccattat gcttcggtca
    66841 tgtcggcaga atatgcccgg aagttagaga aagaagatcg ccaggagcaa ctcgaccgtc
    66901 agccggtcgg cactggaccg tatcagttgt cggaataccg cgccgggcaa tatattcgct
    66961 tacaacgtca tgatgatttc tggcgtggta agccgttgat gccacaagtg gtggtggatt
    67021 taggctctgg cggtacagga cgattgtcga aactcctgac cggggagtgc gacgttctgg
    67081 cctggcctgc tgccagtcag ctatccattt tgcgtgacga cccacgcttg cgtttaacgc
    67141 tgcgcccggg gatgaacgtc gcctatctgg cgtttaacac cgctaaaccg ccgctaaata
    67201 accccgctgt ccgccatgcg ctggcactgg cgattaataa ccagcgcctg atgcaatcca
    67261 tctattatgg tacggctgaa acggcggcct ctattttacc gcgcgcctcg tgggcctatg
    67321 acaacgaggc taaaattact gaatacaatc cggcaaaatc gcgcgaacag ttgaaggcgt
    67381 tggggctgga aaacttaacg ctgaaactgt gggtgccaac acgttcgcag gcgtggaacc
    67441 ccagtccgct gaaaaccgcc gaactgattc aggcggatat ggcacaggtt ggcgtaaaag
    67501 tggtgattgt gccggtagaa ggtcgttttc aggaggcgcg gttgatggat atgagccatg
    67561 atctgacgtt atccggttgg gcgacggaca gtaacgaccc ggacagtttc ttccgtccgt
    67621 tactgagctg cgcggcaatt cattcacaga ccaacctcgc ccactggtgc gatccgaaat
    67681 tcgacagcgt gttgcgtaag gcgctctcct cgcagcagct ggcggcgcgt attgaagcct
    67741 atgacgaagc gcaaagtatt ctggcgcagg aattacctat tctgccgctg gcgtcgtcat
    67801 tgcgtttgca ggcctatcgg tacgatatca aaggtctggt acttagcccg tttggtaacg
    67861 cctcctttgc tggggtgtat cgcgagaaac aggatgaggt gaaaaagcca tgattatctt
    67921 caccttacgc cgcattttgt tattgattgt caccttgttc ctgctgacct tcgttggctt
    67981 cagcttgagc tatttcaccc cacatgcgcc gttacaaggc gcgtcactgt ggaatgcctg
    68041 ggtgttctgg tttaatggcc tgatccactg ggattttggt gtttccagca ttaacggtca
    68101 accgattgcc gaacagttaa aagaggtctt cccggcgacg atggaactgt gcatcctcgc
    68161 cttcggtttt gcgctgattg ttggcatccc cgtgggaatg attgccggga ttacgcgcca
    68221 taagtggcag gataacttga tcaacgccat cgccttactg ggcttttcaa tcccggtgtt
    68281 ctggctggcg cttctgctga cgctgttttg ttcactcacg ctaggttggt tgcccgtttc
    68341 tgggcgtttc gatttgctct acgaagtgaa accgattacc ggttttgcgt tgattgatgc
    68401 ctggctttcg gactcaccgt ggcgggatga aatggtcatg agcgcaatcc gccatatgat
    68461 attacccgtg atcaccctgt cggttgcgcc aacaactgaa gtgatccgac tgatgcgtat
    68521 tagcactatc gaagtgtatg accagaacta tgttaaagca gcggcgaccc gcggtttgtc
    68581 gcgctttact attttgcgtc gccacgttct gcataacgcg ttacctccgg ttattcctcg
    68641 cctgggctta cagttttcta ccatgctgac gctggcgatg atcaccgaaa tggtttttag
    68701 ctggccgggt ttagggcgct ggttaattaa cgccatccgc cagcaggatt acgcagccat
    68761 ttccgccgga gtgatggtgt gtggctcact ggtcattatt gttaacgtga tttctgatat
    68821 tttgggtgcc atggctaacc ctctgaaaca taaggaatgg tatgccttac gatagcgtat
    68881 acagcgaaaa gcgcccgccg ggcacgctgc gtaccgcctg gcgcaaattt tatagtgacg
    68941 cctctgcgat ggtcggcctg tacggctgcg cggggctggc tgtactgtgt atttttggcg
    69001 gctggtttgc gccttacggc atcgaccagc aatttctcgg ttaccaatta ttgccgccgt
    69061 catggtcgcg ctatggcgaa gtttctttct tcctggggac tgacgacctc ggacgcgatg
    69121 ttttaagccg tttactgagc ggagctgcgc caaccgtagg tggcgcattt gtggtgacgc
    69181 ttgccgcgac gatctgcggc ctgatactgg gcaccttcgc cggcgcgacg cacggcctac
    69241 gctcagcggt acttaaccat attctggata ccttactggc gatcccttcc ctactgctgg
    69301 caattatcgt tgtagcgttt gccggaccga gtttgtctca cgccatgttt gccgtctggc
    69361 tggcgctgct gcctcgtatg gtacgttcga tttacagcat ggtgcatgac gaactggaaa
    69421 aagagtacgt tatcgccgcc cgtctggatg gcgcatcaac gctgaatatt ctctggtttg
    69481 ctgtgatgcc caacatcacc gccgggctgg tcaccgagat cacccgcgca ctgtcgatgg
    69541 caattctcga tatcgccgcg ctgggctttc tcgatctcgg cgcacaactc ccctcgcctg
    69601 aatggggagc gatgctcggt gatgcgctgg aactgattta tgtcgccccg tggactgtca
    69661 tgctgccagg tgcggcaatt atgattagcg tgctgttggt taacctcctt ggcgacggtg
    69721 tccgtcgtgc aattattgcg ggggtggaat aatgccatta ctcgatattc gtaacctgac
    69781 cattgaattt aaaaccggtg atgagtgggt taaagccgtc gaccgcgtaa gtatgacctt
    69841 aaccgaaggt gaaatccgcg gtcttgttgg cgaatccggt tccggcaaaa gtttgattgc
    69901 taaagcgatt tgtggggtga ataaagataa ctggcgtgtt actgctgacc gtatgcgttt
    69961 tgatgatatc gatttgctgc gtctctccgc acgcgaacgg cgcaagctgg ttggtcacaa
    70021 cgtgtcgatg attttccagg aaccacagtc gtgtcttgac ccttcagaac gtgtgggtcg
    70081 ccagttgatg caaaacatcc ctgcctggac ctataaaggc cgttggtggc agcgttttgg
    70141 ctggcgcaaa cgccgtgcca ttgaactgct gcaccgcgtg gggattaaag atcacaaaga
    70201 tgcgatgcgc agttttccct atgagttgac cgaaggtgaa tgtcagaaag tgatgatagc
    70261 cattgcactg gcgaatcaac cgcgtctgct aattgctgac gaaccgacca actcaatgga
    70321 gccaacaacc caggcgcaaa tctttcgcct gctgacgcgt ctcaaccaaa acagtaatac
    70381 cactattttg cttatcagcc atgacttaca aatgcttagc caatgggcgg ataaaattaa
    70441 cgtgctttac tgcggtcaaa cggtggaaac cgcgccgagt aaggagttgg tgacgatgcc
    70501 acaccatcct tatacccagg cgctgatccg cgcgatacca gacttcggca gcgcgatgcc
    70561 gcataaaagt cgcctcaata cgctgcccgg cgctatcccg ctgctggaac agttaccgat
    70621 tggttgtcgt ctggggccac gttgcccgta tgcacaacga gaatgcattg tgacgccacg
    70681 tttgacgggg gcgaaaaatc atctctatgc ctgtcatttc ccactgaaca tggagaaaga
    70741 gtgagatgat cgaaacgcta cttgaagtgc gcaatttaag taaaacgttc cgctaccgga
    70801 ccggctggtt tcgtcgtcag accgtagaag cggtaaaacc cttgagcttt acgctacgtg
    70861 aaggccagac actggcgatt atcggcgaga atggttcggg taaatccacg ctggcaaaaa
    70921 tgctggcggg aatgatagaa cctaccagcg gcgagttatt gattgacgat catccgctgc
    70981 attttggcga ttattccttc cgcagtcagc gtattcgcat gatttttcag gatccttcga
    71041 cctcgttgaa tccccgtcaa cgtatctcgc aaattctgga ttttccactg cgcctgaaca
    71101 ccgacctgga accggaacag cgtcgcaaac agattattga aacgatgcgt atggttgggc
    71161 tgctaccgga tcacgtcagt tactatccgc atatgttagc acccgggcaa aaacagcgtc
    71221 tgggtctggc gcgcgcgctg atattgcgcc caaaagtcat tattgccgat gaagccctcg
    71281 cctcactgga tatgtcgatg cgttcgcagt tgattaatct gatgctggag ttacaggaaa
    71341 aacagggcat ttcgtatatt tatgttaccc agcatatcgg aatgatgaag cacattagcg
    71401 accaggtgct ggtgatgcat cagggtgagg ttgtcgagcg aggcagcacc gcagatgtgc
    71461 tggcatcgcc gctgcatgaa ctcaccaaac ggctgatcgc cggtcatttt ggcgaggcat
    71521 tgacggcgga tgcgtggcgt aaggaccgtt agtgttttgc acctcatcct gacaggcccc
    71581 cctcaggatg aggtgttcat tacttttggt tgtcgattct ggtgagagca accagcaatg
    71641 ccagcgtata acacaccgta attgccagac tcatttttaa cagtgtttct ccgccaattc
    71701 ccgacaatgg cgcggctata ccgccgaaga caaacatgct catccccatc aacgcagacg
    71761 ccgttccaga ttcctgcgtc cctactgcac tcatagcctc tgccccgcca acagtgccga
    71821 tgccgctaca aaatgcgatg gtgaaaaata atgccaccag tgccggaatg ggcaattgta
    71881 tccatgcgca taagaccgtg agcaatgcac acaaaattgc cgctatcagg ccacctcgca
    71941 acaatgtcat cgcgttaatc cgtcgcgcca ggcgcgagaa gatccatgaa gcaatgataa
    72001 gtccgatgcc gttaaggcca aacaccaggc taaattgcat tggactaaag ccaaattcct
    72061 tctgcaacac gaacgacgaa gagccgatat atgcaaacaa accagccagc ataaaacttt
    72121 gaatcaggca aaagcgtata aaacggcggt tctgcaccac gcttcgtccg gttaatagca
    72181 atgatgagcc ccttttattt tctggcaagg tctcattaat aaataacagg cagcccagca
    72241 acagtacggt gctaatttca gccattaccc agaataaagt gcgccagtca aaagtgctaa
    72301 caatgtaccc gcccagcact ggcgacaaca ccggtgccag gccattcacc gtcattaaca
    72361 gcgcaaaaaa ctgggtcaac gttactccct gatatttgtc acgagcaata gaacgagaga
    72421 gcaccgaacc acccgccccc gcgatccctt gaataaaacg ccagaccacc agccagtaaa
    72481 tattgttcgt tgtcgcgcat aaaatggaag aaacaataaa caatagcaac gacaagatta
    72541 acggacgttt acgccctatt ttgtcgctca gagggccaaa taacagttgt ccgacgccaa
    72601 gaccaatcag tgatgcggtc agggttaatt gcgttatggt cgtggttgcc gccagttgct
    72661 cagacatctc cggcaaagcc ggcaaataaa gatcggtaca catcgggccg ataccggcta
    72721 aaagaccaag aatcaatgcc catgaaagag agactctagc catattattc cttacttgag
    72781 tgatgacatg ccgccgccca gcgactgcca gagcgtgatt cggtttgtaa aatcagtcag
    72841 ttgcagtgcc agcagctcct gctgtgcgct ccacaagctg cgctgcgccg tcaacacggt
    72901 caggtaatca ccaacacccg cctgatagcg acgcaagcca acatcgaccg tttgttgttc
    72961 agctttaaca tactggcgct gtgcatccag ttgctcttca agcgtgttcc gccgcgccag
    73021 tgcgttagcg acatctttaa atgcgctctg aacggttttt tcataggtgg caatgagtcc
    73081 acgcttttgt gcttctgcgt aacgaagctg cgccagatta cttccgccag taaacagcgg
    73141 taatgtgacc gatggcgcaa atgaccagat ctgcattccg tggctgaaca acgacgacaa
    73201 cgcgtcactg ccaacaccgg cactggcagt cagcgaaatc gtcgggaaaa agttagcccg
    73261 cgccgcgcca atatctgcat tggcgctctt caaattatgt tccgcttcct ggatatccgg
    73321 gcgacgcagc agaacatcag atgatactcc cgcaggaacc agacttatca tttgttccgg
    73381 caggctttcc agtgttcccg gtagcagatt ttccgcaagc gttgtacctg ccagtaagtt
    73441 cagcgcattt ttatcctgca tcacttgtgt ctgatagctg gcaacgctgg cgcgcgcttg
    73501 ctgataaaca ctcatcgcct cactgacatc tgtcgctgcc gccgtgccaa cctgttgctg
    73561 gcgctggata attttcaatg agttttctgc actggccatc gtttcttttg ccagggccag
    73621 gttgctgtta tcggccgcca gtgtcagcca ggcggtgctg atttcagcaa tcagcgttaa
    73681 acgagtgttc tgggctgtaa attcactggc aagccaggtt tcccgcgccg cccgagataa
    73741 actctgatta cgaccaaaca gatctaacgt ataactggac accgtgccgt cagcctcggc
    73801 agttgttccc gtaccattcg ccagcgaacg actgcgagtc gaacttaagg ccgcattcac
    73861 cgtcggaaat aacgacgcat tagtttgccc atagagcgcc cgtgctgagt caatatcagc
    73921 aatcgctttt tgcacatcgc ggttactgtt cagtgcaatg gttactacct gctgcaatcg
    73981 gggatcgtgg atcacctgct gccagtcatg gctgattgcc ttcccctggc cctgtgctcc
    74041 cggcaaggtc gccgggattg gggattcagg cgtactgtag tgaggatcga gcgagacaca
    74101 tcccgcactc aacaacacca gaaaaataag agaacgacgc aacataactt actcctgacg
    74161 gcgcggttta ccggcaaaca aacgtttcac cagtacaaaa aacagaggaa cgaagaaaat
    74221 ggccagtaat gtagcggtca gcgtgccgcc aataatgccc gtaccaatgg cgatgcggct
    74281 gttcgcccct gcgccggttg ctatcgccag cggcattacc cccgcaataa acgccagcga
    74341 ggtcatgatg attgggcgta aacgagtctg cgccgcgcgt aatgccgcac gactcaagga
    74401 atagccctct gcaaccgccg cttcagcaaa ttccacaatc agaatggcgt ttttcgacga
    74461 caagccgata gtagttaata gcgctacctg gaagtaaacg tcgttgttta aatcacgcat
    74521 ccaggccgcc agcgccgcgc caagaagccc cagagggatc accagaatga ccgagaacgg
    74581 gactgaccag ctttcgtaca gtgctgcaag gcagaggaaa accaccagaa tagaaactgc
    74641 atacagactt aatgcctgac cgctggccag tttctcctgc aatgacaaac cactccaggc
    74701 ccaggttgtt cccgcaggaa gactgtttgc cagttcctcc atttttgtca ttgcatcgcc
    74761 ggaactaaag ccagtcgcat tttccccctg aatttcatag gctgcagaac cgttgtagcg
    74821 caccagtctt tccggtccat acagccagcg ggtggtcgca aaagcagaga atggtgtcat
    74881 ggcgttatcg ctaccgcgca caaaccattt acctaagtct gacggcgcgg aacgaaattc
    74941 gctgtcgccc tgaatgtaga cttttttcac acgaccacgg tcaataaagt cattcacata
    75001 agtaccgccc cacgcactgg acagggtgtc ggtgacatca ttcaaactca accctaatga
    75061 caccgcttta ttactatcaa tatctacctg caattgcggc atttgtggca aatcattggc
    75121 gcgtacagaa tgtaactccg gactttgatt cgctttttct atcaactgat tacgcatttg
    75181 cagcagtgtt tcacgatcgg ttccaccgtt agctaacaat tcaaacgtaa aaccattgct
    75241 ttggcccagc ccatcaacgg ctggcggcgt catcgcaaat accgtggcat cacgaattgt
    75301 gcccagctct ttggtagccc gtagggcgat agcctgggcg gtgttttctg cccctttacg
    75361 ttgagaccag tttttcaacg aaacaaacgc catcccggtg ttctgtccgc tgccgctaaa
    75421 actgaaacca tcaacggtaa aaatgacatc ggtatttgct ttctcgttaa tcaggaacca
    75481 gtcaacaatc tggcgattca cttctgctgt acgggcagcg gtcgccccgg ccggcagcgt
    75541 gtactgcacc atgatttcgc cctggtcttc agtgggtaag aaactgcccg gcagtttcca
    75601 catcatcagg gccattccac cacccagtac gacataaagc cccatcgttc gagcagcacg
    75661 gcgcaggaca taaattacgc ctcgctgata tttatcttca gtacggcggt aaaagcggtt
    75721 aaatgcgccg aaaaagcctt ttttatgtgg cggaacatgc tggaggacgg aaccgcacag
    75781 ggcgggagtc aaggtcaatg ccaccaccac ggaaagcagc attgcggaga tgatggtgat
    75841 ggagaactga cgataaatta cgcccgtaga tcccccaaag aaggccatcg gcaggaatac
    75901 ggctgacaac accagcgcaa tggcaaccag tgcgccagaa atctcgccca ttgatttttc
    75961 cgtggcttca cgcgcgggca acccttcgtc acgcataatg cgttcgacgt tctctaccac
    76021 cacaatggca tcgtccacca gcaagccgat cgccagcacc attgcaaaca acgtcagggt
    76081 attaatcgaa tagccaaaca acgcaagaac gccgaatgtg cccagcaaaa cgacggggac
    76141 agctaacgcc ggaatcagtg tggcacgcag gttttgcagg aataaataca tcacgcaaac
    76201 caccaatacg atggcttcaa acagcgtctg aattacgtcc tctacggaga ttttaataaa
    76261 ttcagtgctg tctttcgggt atgcgatgtc gtatccctgc ggcatgttcc gctggaattc
    76321 ggcaatctta tccttgacca gcgtcgccgt attcagcgca ttcgcaccgg gcgaaagcat
    76381 tacagcaacc ccggcagacg ggtgaccgtt aagtttgccg atagcggtat aatcttcact
    76441 gcccatctca acgcgagcca catcttttat acgaacaact gcgccgtctg actggctttt
    76501 cacgataata tttttgaact gatccaccgt ctgcaaacga gactgcgcac gtaccgttgc
    76561 ggtcagttgc tgagtattcg gtgaaggcaa tgcccctatt ttcccggcag taacctgcac
    76621 gttttgcgct tcaatagcac tttgcacgtc tgaaggcatt agcgagtaag acgccagttt
    76681 ggccgggtca agccagatgc gcatagcgta ttccgcgcca aagacttgca gactccccac
    76741 accttcaaca cgcgccagcg ggtcctgaac gttactaacc aaccagtcgg cgatatccga
    76801 actggaggct ttgtcagtgg tgtcatacac ggcagcaatc agcaaaaagt tgctttgtga
    76861 tttctccacc gtaacacccg tttgctgcac ttcgctgggt aggcgcgact ccgcctgctg
    76921 aattttattc tgcacctgaa cctgtgcagt atctggatcg gtaccttgtt caaaggtcac
    76981 attaatactg accgaaccat cagagctgct ggttgagctg aaatagagta aattatcaag
    77041 cccagtgagt tgttgctcga taacctgagt cacgctgttc tccagcgttt cggcagaagc
    77101 accagtataa gtggctgaaa ttttaatggt cggtggcgca acgtcaggat attgcgcgac
    77161 gggcaatgtg cgaatagcca gaatccccgc caacataatc aaaatggcga taacccaggc
    77221 aaagaccggg cggcgaacaa aaaagcgcga aaacatcagg cgttgcctcc attagcctga
    77281 acttcaacag ccttgacggt ctgccctgaa gtgactttgg cagaaccttc aacaatcagt
    77341 cggtcgccgt tgtgcaggcc gttcagcacc agccatttat caccatacgt ttctcccgtt
    77401 tcgagcgttc gctgctctac tttattgtct ttattcacca ccagcgcagt tgcattgcct
    77461 ttagcatcgc gcgtgacgcc ctgttgcggc gcgagaatag cgtcttccat cacgccttca
    77521 tcgacacgag cgcgaacaaa cattcccggt aataactgct gttgtggatt gggaaaaatc
    77581 gcccgtaatg tcaccgaacc ggtagactca tctacagcga cttcggtgag ttccagacgc
    77641 cctttttcgc tgtaagttgt gccatcttcc agaataagtg agacgctcat ggtgtcactg
    77701 ttggtcgcca gtgactgttt acgcagacgt aataaatcga cactagagcg agtgaggtcg
    77761 acatacattg tatccagacc acgaatagtc gttaacgctg tatcctgcga cgcggtcacc
    77821 agtgcgccag gggttaccga cgaaatacca atacgccccg aaatcggtgc ggttaccgtg
    77881 gtccagtcaa gattaatgcg cgcagtttcc agtgcggctt ttttcgcctc tacactggct
    77941 ttatcttgtg cacaggtaga ctgagcatca tcagcatcct gttgtgaaac accgttctct
    78001 ttcaccagtc gggcataacg ctgcgctttc tggcaatcgg cttttaccag tgcctgtgct
    78061 tgttgtaatg ctgcccgagc ttcattccat gcagcctgat aactggccgc atcaatctgg
    78121 tagagcggct gtccagcctt gaccagatca ccttccttaa ataagcgttt ctggataatt
    78181 cccccaacct gcggacgtac ttcagcactg agcgcagcac tggtgcgtcc ggttaattca
    78241 ctgactaccg aaaccggttg agatttgagc gtaacaacgc cgacttctgt ttctgacggg
    78301 gatgaattgt tactttgcgt attatcacaa cccgataaga gcagcattgc cactacagat
    78361 gttgctatat acttcattat tttctccagg gtgagaaata ccctcctcat tgaaattaat
    78421 gaggatatgt cagggatatt cagttataac gaacccgcat cgcgaaatga atttcgcaat
    78481 gaaataaggg ggattaatat cccccgctat tggttaattc tgttcttatt tatttatcaa
    78541 catactaaca atatcctgat atatttcctg cagatgttgt ggatcgcttt tttgcggtgt
    78601 taaacgacga taaaccgtac cttccatcat ggtagcaaac acttcaacgc agcaacgaat
    78661 gtgctcgtcg ctaagatggg gatgcattct tttcatatga gcacaaccgt tagcgaacat
    78721 tctggcgtca gcttcttcaa gcattgccat cacccgcggg ttgcgtgtgg cttctgcagc
    78781 cacttccagc atcagcgcgt cgtcgtcctc atttaaggat ttacgcaagg caagaacttc
    78841 cggaaagtga tcggtacgcg cgtcaatgtc catttgggca atacgaaagt cgatgatacg
    78901 gcgaaccatc tcttcaataa tggcgtcttt gttggcgaag taacgatata tctgacccac
    78961 gctaagctga gcttcgctgg caatttgcga catactggcg gcatgaaatc cgcagcgacg
    79021 aaaacaccga cgggcggcgt taatgatttc atcctgacgc tgtttgtggc gtatctccag
    79081 cttgctggtc atttctgtct cctggcctat cacttggtga gaatgatcat tctcattttt
    79141 gctaattata cgtttctcca aagtaatgga aagtattttt atcttattca tgatgcatat
    79201 tctgataatg aagagattgt ggagattctg tgaagatagt tgacaatatt aagttggggt
    79261 caattcatca gaaagacagc gacttgttgt cgccagttct gttttcatca tcaaacttat
    79321 cggagctaat acgaatagtt tcaaagtcag attctataag atatttcccc aacctccccc
    79381 ttgcaggcgt caggagtaga taacgcaaaa tgctctgtac cgcagcttct ctccgctaca
    79441 gaaagcgcaa ctatagccac ccacagccag gttgattata ataaccgttt atctgttcgt
    79501 actgtttact aaaacgacga atcgcctgat tttcaggcac aacaagcatc aacaataagg
    79561 attaaagcta tgggttttct ttccggtaag cgcattctgg taaccggtgt tgccagcaaa
    79621 ctgtccatcg cctacggtat cgctcaggcg atgcaccgcg aaggagctga actggcattc
    79681 acctaccaga acgacaaact gaaaggccgc gtagaagaat ttgccgctca attgggttct
    79741 gacatcgttc tgcagtgcga tgttgccgaa gataccagca tcgacaccat gttcgctgaa
    79801 ctggggaaag tttggccgaa atttgacggt ttcgtacact ctattggttt tgcacctggc
    79861 gatcagctgg atggtgacta tgttaacgcc gttactcgtg aaggcttcaa aattgcccac
    79921 gacatcagct cctacagctt cgttgcaatg gcaaaagctt gccgctccat gctgaatccg
    79981 ggttctgccc tgctgaccct ttcctacctt ggcgctgagc gcgctatccc gaactacaac
    80041 gttatgggtc tggcaaaagc gtctctggaa gcgaacgtgc gctatatggc gaacgcgatg
    80101 ggtccggaag gtgtgcgtgt taacgccatc tctgcgggtc cgatccgtac tctggcggct
    80161 tccggtatca aagacttccg caaaatgctg gctcattgcg aagccgttac cccgattcgc
    80221 cgtaccgtta ctattgaaga tgtgggtaac tccgcggcat tcctgtgctc cgatctctct
    80281 gccggtatct ccggtgaagt ggtccacgtt gacggcggtt tcagcatcgc tgcaatgaac
    80341 gaactcgaac tgaaataatc gttctgttgg taaagatggg cggcgttctg ccgcccgtta
    80401 tctctgttat acctttctga tatttgttat cgccgatccg tctttctccc cttcccgcct
    80461 tgcgtcagga taacgatttc ctttacgacc aaggagcgcc catggaacaa cgccacatca
    80521 ccggcaaaag ccactggtat catgaaacgc aatccagtac tgcggagtat gacgttctgc
    80581 ctctggtccc ggaagccgca aaggtcagcg atccctttct gctcgacgtg atccttgatg
    80641 aagaaacgct ggcacccttc ctttcatggc tggtccctgc gcgcgttctt gcagtagagt
    80701 tgttccctga cccgcttacc gtgacccgtt cacagacctt caccgcttat gaacgcttgt
    80761 cgacggccct gacggttgct caggtttgcg gcgtccaacg gttatgtaac tactattcgg
    80821 cgcgacttac gccgctcccc gggcctgatt cctccaggga aagtaatcat cggttggcac
    80881 aaatcacgca atatgcccgc caactggcta gctcgccttc tattatcgac aaccgatcgc
    80941 gccagcatct gaatgacgtt ggtcttactg tctgtgactg tgtgatcatt aaccaagtca
    81001 ttggttttat tggctttcag gcgcggacaa ttgcgacatt tcaggcttat ctcgggcatc
    81061 cggtacgctg gttacccggt ctggagatac aaaactacgc cgacgcgtca ctgtttgctg
    81121 atgaatcatt acgctggcga agcagctatg aggtggaaaa actccctgaa gagcacacaa
    81181 aaagttcaac tgcagaactt tgccaactgg ccaacacact ctctctccac cctatttcac
    81241 tttcccttct cgaaaagttg ttaaacagca cacgggtcaa tacacagccg gataatcagc
    81301 ttgcggcgtt gttatgcgcg cggataaatg gcagtcctgc ttgttttgcc gcctgtatga
    81361 attcatcaaa tgaatataaa aaaatcagcc cccttctgcg caagggcgaa aatgaaatta
    81421 accgatgggc tgaccgtcat tctgttgagc gcgctaccgt tcaggcgata caatggctga
    81481 cccgagcacc cgatcgcttt agcgccgccc agttcagccc attactcgaa cacgaaaaat
    81541 catcaacgca gattattaat ctgctggtat ggagcgggct gtgtggctgg ataaatcgct
    81601 taaaaatcgc gttgggtgag acatattaac cttgccgcgt cagacagatt cgcgtaaaac
    81661 tgtcagccgc tctaatggcc atcaaaatag acaattatgt ttcaggacaa cccgctgcta
    81721 gcgcagctta aacagcaact gcattcccag acgccacgcg ttgaaggggt ggtaaaagcc
    81781 acagaaaaag gctttggctt cctggaagtc gacgcgcaaa aaagttattt cattccgccg
    81841 ccgcagatga aaaaagtcat gcatggcgac cgaattatcg cggttatcca cagtgaaaaa
    81901 gaacgtgaat ccgcagagcc agaagaactg gttgaaccgt tcctgactcg tttcgtgggt
    81961 aaagttcagg gcaaaaatga ccgtctggcc atcgttcctg atcatccact cttaaaagac
    82021 gccattcctt gccgcgcagc ccgtggcctg aaccacgagt ttaaagaagg cgactgggcg
    82081 gttgccgaaa tgcgccgtca tccgctgaaa ggcgatcgtt ctttctatgc tgaactgaca
    82141 caatacatca cttttggtga cgatcacttt gtaccgtggt gggttaccct tgcgcgtcat
    82201 aatctggaaa aagaagcacc agacggcgtc gctaccgaaa tgctcgatga aggtctggtt
    82261 cgtgaagatc tgactgagct ggattttgtc accatcgaca gtgccagcac agaagatatg
    82321 gatgacgccc ttttcgccaa ggcgttaccg gatggcaaac ttcagctgat tgtggcgatt
    82381 gccgatccaa ccgcgtggat tgctgaaggc agcaagctgg acaaagccgc gaaaattcgc
    82441 gcattcacca actatctgcc tggcttcaac atccctatgc tgcctcgcga gctttctgac
    82501 gatctctgct cactgcgcgc caatgaagtc cgcccggtac tggcatgccg catgacgctc
    82561 tccactgatg gcaccattga agataatatc gaattctttg ccgccaccat cgaatccaaa
    82621 gcgaagctgg tgtatgacca ggtttctgac tggctggaaa ataccggtga ctggcagccc
    82681 gaaagtgaag caattgccga acaagtccgt ttgctagcgc aaatttgcca acgccgcggc
    82741 gagtggcgtc ataaccacgc actggtgttt aaagatcgcc cggattaccg ctttattctc
    82801 ggtgaaaaag gtgaagtgct ggatatcgtc gccgagcctc gtcgcattgc caaccgtatc
    82861 gtcgaagaag cgatgattgc cgctaatatt tgtgcggccc gcgtactgcg cgataagctc
    82921 ggttttggca tctataacgt gcatatgggc tttgatccgg cgaatgccga cgcgctggca
    82981 gcgttgctga aaacgcacgg tctgcatgtc gatgctgaag aagtgctcac gctggacggt
    83041 ttctgcaaac tgcgtcgtga actggatgcg caaccaactg gtttcctcga cagccgcatt
    83101 cgtcgcttcc agtcatttgc tgaaattagc actgaacccg gtcctcactt tggcctcggt
    83161 ctggaagcat acgccacctg gacttcgccg atccgtaaat atggcgacat gatcaaccac
    83221 cgtctgctga aagcggttat caaaggcgaa actgcgacgc gtccgcagga tgaaatcact
    83281 gtccaaatgg ccgagcgtcg ccgtctcaac cgaatggcag aacgtgatgt tggcgactgg
    83341 ttatacgcac gcttcctgaa agacaaagcc gggaccgaca cccgtttcgc ggcggaaatt
    83401 gtcgatatca gccgtggcgg catgcgtgtg cgtttggttg ataacggcgc tatcgccttt
    83461 attccggcac ctttcttaca cgctgtgcgc gatgaactgg tttgcagcca ggaaaacggc
    83521 actgtacaaa ttaaaggtga aacggcttac aaagtgactg acgttattga cgtcaccatt
    83581 gccgaagtcc gcatggaaac ccgcagc
//
LOCUS       DAPHCV010000003        79211 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.003, whole genome shotgun sequence.
ACCESSION   DAPHCV010000003 DAPHCV010000000
VERSION     DAPHCV010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 79211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.003"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            90..533
                     /gene="cmtB"
                     /locus_tag="RH033_000166"
     CDS             90..533
                     /gene="cmtB"
                     /locus_tag="RH033_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HDV2989413.1"
                     /translation="MRLIDYFPESSISVIHSAKDWQEAIDFSMASLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            561..1949
                     /gene="cmtA"
                     /locus_tag="RH033_000167"
     CDS             561..1949
                     /gene="cmtA"
                     /locus_tag="RH033_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDV2989414.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLAGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYIDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSVI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            1964..3241
                     /locus_tag="RH033_000168"
     CDS             1964..3241
                     /locus_tag="RH033_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDV2989415.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVNVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTDKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIVINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYMNTKGMSDPVQTLRALTDDAGFDDVFVYAAVPSVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNRTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDSGEAAQ"
     gene            3238..4203
                     /gene="yggF"
                     /locus_tag="RH033_000169"
     CDS             3238..4203
                     /gene="yggF"
                     /locus_tag="RH033_000169"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HDV2989416.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVVMGQSNALAV
                     MAFAPRGSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDSCQAKGDYTENRQIAEQERKRCKEMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            4225..4734
                     /gene="fumE"
                     /locus_tag="RH033_000170"
     CDS             4225..4734
                     /gene="fumE"
                     /locus_tag="RH033_000170"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HDV2989417.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTTHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     EMPGFADDMTWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSRMVKTALTLAV
                     TTTLKELTP"
     gene            4731..5444
                     /locus_tag="RH033_000171"
     CDS             4731..5444
                     /locus_tag="RH033_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HDV2989418.1"
                     /translation="MKIELTVNGLKIQAQYQNEEIENVHKPLLHMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVMEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WQELASFCDFSIFIHAPAQILRERLISRKIAGGLSRQEAEAFYARTDGPNVERVLVNS
                     RQANLILEMTEEGRYHFKS"
     gene            5729..6748
                     /gene="epd"
                     /locus_tag="RH033_000172"
     CDS             5729..6748
                     /gene="epd"
                     /locus_tag="RH033_000172"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HDV2989419.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            6798..7961
                     /gene="pgk"
                     /locus_tag="RH033_000173"
     CDS             6798..7961
                     /gene="pgk"
                     /locus_tag="RH033_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDV2989420.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            8175..9254
                     /gene="fbaA"
                     /locus_tag="RH033_000174"
     CDS             8175..9254
                     /gene="fbaA"
                     /locus_tag="RH033_000174"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HDV2989421.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            9445..10305
                     /gene="mscS"
                     /locus_tag="RH033_000175"
     CDS             9445..10305
                     /gene="mscS"
                     /locus_tag="RH033_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HDV2989422.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTDIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            10444..11079
                     /gene="argO"
                     /locus_tag="RH033_000176"
     CDS             10444..11079
                     /gene="argO"
                     /locus_tag="RH033_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HDV2989423.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIVHAQALLS"
     gene            11172..11912
                     /locus_tag="RH033_000177"
     CDS             11172..11912
                     /locus_tag="RH033_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HDV2989424.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            12080..12976
                     /locus_tag="RH033_000178"
     CDS             12080..12976
                     /locus_tag="RH033_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2989425.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTSH"
     gene            complement(12973..14451)
                     /gene="scpC"
                     /locus_tag="RH033_000179"
     CDS             complement(12973..14451)
                     /gene="scpC"
                     /locus_tag="RH033_000179"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HDV2989426.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPNAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(14475..15260)
                     /gene="scpB"
                     /locus_tag="RH033_000180"
     CDS             complement(14475..15260)
                     /gene="scpB"
                     /locus_tag="RH033_000180"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HDV2989427.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDADFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(15271..16266)
                     /gene="meaB"
                     /locus_tag="RH033_000181"
     CDS             complement(15271..16266)
                     /gene="meaB"
                     /locus_tag="RH033_000181"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HDV2989428.1"
                     /translation="MINEATLAESIRRLRHGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNRLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(16259..18403)
                     /gene="scpA"
                     /locus_tag="RH033_000182"
     CDS             complement(16259..18403)
                     /gene="scpA"
                     /locus_tag="RH033_000182"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HDV2989429.1"
                     /translation="MSKVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYLPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSAAE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGRKDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(18607..19500)
                     /gene="argP"
                     /locus_tag="RH033_000183"
     CDS             complement(18607..19500)
                     /gene="argP"
                     /locus_tag="RH033_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HDV2989430.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            19641..19826
                     /locus_tag="RH033_000184"
     CDS             19641..19826
                     /locus_tag="RH033_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2989431.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFASWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            19882..20541
                     /gene="rpiA"
                     /locus_tag="RH033_000185"
     CDS             19882..20541
                     /gene="rpiA"
                     /locus_tag="RH033_000185"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDV2989432.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            20797..22029
                     /gene="serA"
                     /locus_tag="RH033_000186"
     CDS             20797..22029
                     /gene="serA"
                     /locus_tag="RH033_000186"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDV2989433.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            22258..22317
                     /gene="ibsC"
                     /locus_tag="RH033_000187"
     CDS             22258..22317
                     /gene="ibsC"
                     /locus_tag="RH033_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HDV2989434.1"
                     /translation="MMRLVIILIVLLLISFPAY"
     gene            complement(22418..23020)
                     /locus_tag="RH033_000188"
     CDS             complement(22418..23020)
                     /locus_tag="RH033_000188"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDV2989435.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTLPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(23041..23224)
                     /gene="ssrS"
                     /locus_tag="RH033_000189"
     ncRNA           complement(23041..23224)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="RH033_000189"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(23266..23595)
                     /gene="zapA"
                     /locus_tag="RH033_000190"
     CDS             complement(23266..23595)
                     /gene="zapA"
                     /locus_tag="RH033_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDV2989436.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            23763..24341
                     /gene="ygfB"
                     /locus_tag="RH033_000191"
     CDS             23763..24341
                     /gene="ygfB"
                     /locus_tag="RH033_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HDV2989437.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            24367..25692
                     /gene="pepP"
                     /locus_tag="RH033_000192"
     CDS             24367..25692
                     /gene="pepP"
                     /locus_tag="RH033_000192"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HDV2989438.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYTYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSMQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            25689..26867
                     /gene="ubiH"
                     /locus_tag="RH033_000193"
                     /gene_synonym="visB"
     CDS             25689..26867
                     /gene="ubiH"
                     /locus_tag="RH033_000193"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HDV2989439.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGCVLVA
                     ADGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQYGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQRDREATIGVTDSLVHLFANRWGPLVVGRNIGLMAMELFTPARDVLAQRTLGWVAR
                     "
     gene            26890..28092
                     /gene="ubiI"
                     /locus_tag="RH033_000194"
                     /gene_synonym="visC"
     CDS             26890..28092
                     /gene="ubiI"
                     /locus_tag="RH033_000194"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HDV2989440.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQAGEDEFNRALNIAFDNRLGLCKVESERLVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(28166..28249)
                     /locus_tag="RH033_000195"
     CDS             complement(28166..28249)
                     /locus_tag="RH033_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2989441.1"
                     /translation="MSKNTKSKNHDIRKYNAKTEVKLVYFK"
     gene            28540..29634
                     /gene="gcvT"
                     /locus_tag="RH033_000196"
     CDS             28540..29634
                     /gene="gcvT"
                     /locus_tag="RH033_000196"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HDV2989442.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYSQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            29658..30047
                     /gene="gcvH"
                     /locus_tag="RH033_000197"
     CDS             29658..30047
                     /gene="gcvH"
                     /locus_tag="RH033_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDV2989443.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            30166..33039
                     /gene="gcvP"
                     /locus_tag="RH033_000198"
     CDS             30166..33039
                     /gene="gcvP"
                     /locus_tag="RH033_000198"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HDV2989444.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMLPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(33203..34636)
                     /gene="bglA"
                     /locus_tag="RH033_000199"
     CDS             complement(33203..34636)
                     /gene="bglA"
                     /locus_tag="RH033_000199"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HDV2989445.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFDWYKEVIASNGENL"
     gene            34681..34992
                     /locus_tag="RH033_000200"
     CDS             34681..34992
                     /locus_tag="RH033_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDV2989446.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTDLKKVITDIYPGQTQFYVIEFKC
                     L"
     gene            35156..35815
                     /locus_tag="RH033_000201"
     CDS             35156..35815
                     /locus_tag="RH033_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDV2989447.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     IKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(35892..36872)
                     /gene="ygfZ"
                     /locus_tag="RH033_000202"
     CDS             complement(35892..36872)
                     /gene="ygfZ"
                     /locus_tag="RH033_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HDV2989448.1"
                     /translation="MAFTPFPPRQPTASARLPLTLITLDDWALATITGADSEKYMQGQ
                     VTADVSQMTENQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            37125..37391
                     /gene="sdhE"
                     /locus_tag="RH033_000203"
     CDS             37125..37391
                     /gene="sdhE"
                     /locus_tag="RH033_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HDV2989449.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            37372..37779
                     /locus_tag="RH033_000204"
     CDS             37372..37779
                     /locus_tag="RH033_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HDV2989450.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(37819..38340)
                     /gene="fldB"
                     /locus_tag="RH033_000205"
     CDS             complement(37819..38340)
                     /gene="fldB"
                     /locus_tag="RH033_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HDV2989451.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            38452..39348
                     /gene="xerD"
                     /locus_tag="RH033_000206"
     CDS             38452..39348
                     /gene="xerD"
                     /locus_tag="RH033_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDV2989452.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            39373..40083
                     /gene="dsbC"
                     /locus_tag="RH033_000207"
     CDS             39373..40083
                     /gene="dsbC"
                     /locus_tag="RH033_000207"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HDV2989453.1"
                     /translation="MKKGFMLFTLLAAFSGFVQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            40089..41822
                     /gene="recJ"
                     /locus_tag="RH033_000208"
     CDS             40089..41822
                     /gene="recJ"
                     /locus_tag="RH033_000208"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDV2989454.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDPQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYRLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            41913..43011
                     /gene="prfB"
                     /locus_tag="RH033_000209"
     CDS             join(41913..41987,41989..43011)
                     /gene="prfB"
                     /locus_tag="RH033_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDV2989455.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            43021..44538
                     /gene="lysS"
                     /locus_tag="RH033_000210"
     CDS             43021..44538
                     /gene="lysS"
                     /locus_tag="RH033_000210"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDV2989456.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(44581..45129)
                     /gene="idi"
                     /locus_tag="RH033_000211"
     CDS             complement(44581..45129)
                     /gene="idi"
                     /locus_tag="RH033_000211"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDV2989457.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQQGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQATNREARKRLSAFTHLK"
     gene            complement(45251..45376)
                     /locus_tag="RH033_000212"
     CDS             complement(45251..45376)
                     /locus_tag="RH033_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2989458.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(45378..46826)
                     /gene="uacT"
                     /locus_tag="RH033_000213"
     CDS             complement(45378..46826)
                     /gene="uacT"
                     /locus_tag="RH033_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HDV2989459.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILIMFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            47262..49181
                     /gene="ygfT"
                     /locus_tag="RH033_000214"
     CDS             47262..49181
                     /gene="ygfT"
                     /locus_tag="RH033_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HDV2989460.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIDVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYYGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHTMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFNTKAS"
     gene            49181..49669
                     /locus_tag="RH033_000215"
     CDS             49181..49669
                     /locus_tag="RH033_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDV2989461.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(49705..51072)
                     /gene="ghxQ"
                     /locus_tag="RH033_000216"
                     /gene_synonym="ygfQ"
     CDS             complement(49705..51072)
                     /gene="ghxQ"
                     /locus_tag="RH033_000216"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HDV2989462.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(51108..52424)
                     /gene="guaD"
                     /locus_tag="RH033_000217"
     CDS             complement(51108..52424)
                     /gene="guaD"
                     /locus_tag="RH033_000217"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HDV2989463.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(52442..53842)
                     /gene="xanQ"
                     /locus_tag="RH033_000218"
     CDS             complement(52442..53842)
                     /gene="xanQ"
                     /locus_tag="RH033_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HDV2989464.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(54007..56877)
                     /locus_tag="RH033_000219"
     CDS             complement(54007..56877)
                     /locus_tag="RH033_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HDV2989465.1"
                     /translation="MIIHFTLNGAPQELTVNPSENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIDAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEDIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEEDNR
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGEIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(56874..57653)
                     /locus_tag="RH033_000220"
     CDS             complement(56874..57653)
                     /locus_tag="RH033_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HDV2989466.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVETQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(57704..59032)
                     /gene="ssnA"
                     /locus_tag="RH033_000221"
     CDS             complement(57704..59032)
                     /gene="ssnA"
                     /locus_tag="RH033_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HDV2989467.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRNAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPGSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(59035..62133)
                     /gene="ygfK"
                     /locus_tag="RH033_000222"
     CDS             complement(59035..62133)
                     /gene="ygfK"
                     /locus_tag="RH033_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HDV2989468.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDALNKLLQDEAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWELNHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVADHGVKFEYGCSPD
                     LTIEQLKNQGFHYVLIATGTDKNSGVKLAGDNPNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISVTLVDSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(62455..63033)
                     /gene="mocA"
                     /locus_tag="RH033_000223"
     CDS             complement(62455..63033)
                     /gene="mocA"
                     /locus_tag="RH033_000223"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HDV2989469.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     EHYSVEIENAEIILDIDTPDDFITAKKRYTEI"
     gene            63137..63907
                     /gene="yqeC"
                     /locus_tag="RH033_000224"
     CDS             63137..63907
                     /gene="yqeC"
                     /locus_tag="RH033_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HDV2989470.1"
                     /translation="MKSIIDPSALFIDLGAQKRPTVISIVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            63955..65580
                     /locus_tag="RH033_000225"
     CDS             63955..65580
                     /locus_tag="RH033_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HDV2989471.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFI
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(65621..66553)
                     /gene="arcC"
                     /locus_tag="RH033_000226"
     CDS             complement(65621..66553)
                     /gene="arcC"
                     /locus_tag="RH033_000226"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDV2989472.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(66601..67986)
                     /gene="hyuA"
                     /locus_tag="RH033_000227"
     CDS             complement(66601..67986)
                     /gene="hyuA"
                     /locus_tag="RH033_000227"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HDV2989473.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGSNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGILAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(68039..69250)
                     /locus_tag="RH033_000228"
     CDS             complement(68039..69250)
                     /locus_tag="RH033_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HDV2989474.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(69308..70504)
                     /gene="dpaL"
                     /locus_tag="RH033_000229"
     CDS             complement(69308..70504)
                     /gene="dpaL"
                     /locus_tag="RH033_000229"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDV2989475.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDII
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(70562..71749)
                     /gene="ygeW"
                     /locus_tag="RH033_000230"
     CDS             complement(70562..71749)
                     /gene="ygeW"
                     /locus_tag="RH033_000230"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HDV2989476.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMKLTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            72230..74008
                     /locus_tag="RH033_000231"
     CDS             72230..74008
                     /locus_tag="RH033_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HDV2989477.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDENRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLALDDAGGTALEEMEKQMIREALSRHNSK
                     KEVADELGIGIATLYRKIKKYELLNT"
     gene            complement(74048..74527)
                     /gene="xdhC"
                     /locus_tag="RH033_000232"
     CDS             complement(74048..74527)
                     /gene="xdhC"
                     /locus_tag="RH033_000232"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HDV2989478.1"
                     /translation="MNHSETITIECTINGIPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(74524..75402)
                     /gene="xdhB"
                     /locus_tag="RH033_000233"
     CDS             complement(74524..75402)
                     /gene="xdhB"
                     /locus_tag="RH033_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HDV2989479.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPVTQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(75413..77710)
                     /gene="xdhA"
                     /locus_tag="RH033_000234"
     CDS             complement(75413..77710)
                     /gene="xdhA"
                     /locus_tag="RH033_000234"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HDV2989480.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVGINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALADDAAPIHNGGN
                     LLKQSSMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALNIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            78124..78876
                     /locus_tag="RH033_000235"
     CDS             78124..78876
                     /locus_tag="RH033_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDV2989481.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSTRKSTAKSTTK
                     TASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYAA
                     GAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGSTD
                     AASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            78955..79028
                     /locus_tag="RH033_000236"
     tRNA            78955..79028
                     /locus_tag="RH033_000236"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:78987..78989,aa:Gly,seq:ccc)
BASE COUNT        19164 a        19624 c        20920 g        19503 t
ORIGIN      
        1 tctcgcgtac ccaattgctt tcattaaacc gcacgagtat tttattttta ccgccggacg
       61 gtaccctaaa aataaaataa ggataattta tgcggcttat tgattatttt ccagaatcat
      121 caatctcagt tatacattca gcaaaagatt ggcaggaagc tatcgatttc tcgatggcat
      181 cattgctgga taaaaactat atcagcgaga attacattca ggcaattaaa gattccacca
      241 ttaacaatgg cccttattat attctcgcac caggcgtggc aatgcctcat gcgcgaccgg
      301 aatgtggggc gcttaaaacc gggatgtctt tgacattact tgaacaaggt gtttattttc
      361 cggggaatga cgaaccaatt aaattactca tcggactctc tgctgccgat gccgattcgc
      421 acattggcgc tattcaggcg ttaagtgaat tactgtgcga agaagaaata ctcgaacaac
      481 tcttaacagc atcatcagaa aaacaattag cggacattat cagccgcgga taattactct
      541 ctcctctaca ggataatatt atggaaaaca agtctgctcg tgcaaaggtc caggcttttg
      601 ggggcttttt gacggcaatg gtcatcccca atattggtgc ttttattgcc tggggtttta
      661 ttactgcgtt atttattccc accggttggc tgcctaacga acatttcgcc aaaattgtcg
      721 gcccgatgat tacctattta ttgcccgtga tgattggttc taccggtggt catctggccg
      781 gcggtaaacg cggcgcggtc atgggcggaa taggtactat tggtgtgatc gtcggcgcag
      841 agatcccgat gttccttggc tcaatgatta tggggccgct cggtggtctg gtgattaaat
      901 atatcgacaa ggcgctggaa aagcggatcc ctgctggttt tgagatggtt atcaataact
      961 tctcattagg tatcgcgggg atgctccttt gtctgctggg ttttgaagtg atcggccctg
     1021 cggtgttaat tgccaatact ttcgtcaaag agtgtattga ggcgctggta catgcgggtt
     1081 atctgccttt gctgtcagtc atcaatgaac cggcgaaagt gcttttcctt aataatgcga
     1141 tcgatcaggg cgtctattac ccgttgggga tgcaacaggc ttcagttaac ggtaaatcca
     1201 tcttctttat ggtggcgtcc aaccctgggc ctggcttagg actgttgctg gcgtttactt
     1261 tgtttggtaa agggatgagt aaacgttctg cgcccggggc gatgattatt cacttcctcg
     1321 gtgggatcca cgaactgtat ttcccgtatg tgctgatgaa gccgctgacc attattgcca
     1381 tgattgcggg cggtatgtct ggcacctgga tgtttaactt actggacggt ggtctggtgg
     1441 ctggtccaag tccggggtct atctttgctt acctggcact gacgccgaaa ggctcgttcc
     1501 tggcgacaat tgccggtgtt acggtaggta ccctggtgtc ctttgctatc acttccgtaa
     1561 tactgaagat ggaaaaaacg gtggaaacgg agagcgaaga tgagtttgct cagtcagcca
     1621 atgcggttaa ggcgatgaaa caagagggtg cattctcgtt aagccgggtt aagcgaatcg
     1681 cctttgtttg cgatgcgggg atgggctcca gcgcgatggg cgcaaccacc ttccgtaaac
     1741 gtctggaaaa agcggggctg gcaattgaag taaaacatta cgcgatagaa aacgtgcctg
     1801 cggatgcgga tatcgtcgtt actcacgcca gtctggaagg gcgcgtgaaa cgtgtgacgg
     1861 ataaaccact gatattgatt aataactata ttggcgatcc aaaactcgac actttattta
     1921 atcaattaac cgctgaacat aaacactgat tacagaggta aaaatgaaaa ccaaagttgc
     1981 tgctatttat ggcaagcggg atgtccgtct gcgcgaattt gagctgccag aaattaccga
     2041 taatgaactg ttagtgaatg taatttctga cagcgtttgt ttatccacct ggaaagcggc
     2101 gttactcggt agtgaacata aacgcgtacc cgacgattta gaaaatcatc cggtcattac
     2161 cgggcatgaa tgtgccgggg ttattgttga agtgggtaaa aatctcaccg acaaatataa
     2221 aaaaggccag cgttttgtat tgcaaccggc gatggggtta ccaagcggat attcagcggg
     2281 ctacagctac gaatattttg gcggtaacgc cacctatatg attattcccg aaatcgtcat
     2341 taatttgggc tgcgtattac cgtatcacgg ctcttatttt gctgcggcgt cactggcaga
     2401 gcctatgtgc tgcattattg gcgcttatca tgccaattat cacaccacgc aatatgttta
     2461 tgagcatcgc atgggcgtta aacctggcgg caatattgcg ctgctggcat gtgctggtcc
     2521 gatgggcatt ggcgctatcg attatgctat taacggcggc atacaaccgt cgcgggtggt
     2581 ggtggtcgat atcgacgaca aacgcctggc gcaggtacaa aagctgctgc cggtggaact
     2641 ggcggccagt aaaggcattg agctggtgta tatgaatacc aaagggatga gcgatccggt
     2701 ccagacgctg cgggcgctga cggatgatgc cggatttgat gacgtatttg tctatgcagc
     2761 ggtgccttca gtcgtcgaga tggctgatga attactggcg gaagatggct gcctgaactt
     2821 ctttgccggg ccgacggata aaaacttcaa agtgccgttt aatttctaca acgtccatta
     2881 caacagaacg catgtcgtcg gtacgtccgg cggttcaacg gacgacatga aagaggcgat
     2941 tgcgcttagc gccacagggc agttgcagcc gtcgtttatg gtgacccata tcggtgggct
     3001 ggatgcggtg ccagaaaccg tgctcaatct gccggatatc ccaggcggta aaaaactcat
     3061 ttataacggc gtgaccatgc cgctcactgc cattgccgat tttgccgaaa aaggcaaaac
     3121 cgatccgctg tttaaagagt tggcgcggct ggttgaggaa acgcacggca tctggaatga
     3181 acaggccgag aaatatctgc tggcacaatt tggcgttgat agcggggagg ccgcgcaatg
     3241 atgtccctgg cgtggccatt atttcgcgtt acggaacagg cagcgttggc tgcctggccg
     3301 caaaccggat gtggcgacaa aaataaaatt gatggcctgg cggtcaccgc gatgcgccag
     3361 gcattaaacg acgtcgcttt tcgtggacga gtggttatcg gcgagggaga gattgaccat
     3421 gcgccgatgc tgtggattgg cgaagaggtg ggcaaaggcg atgggccaga agtcgatatc
     3481 gcggttgacc cgattgaagg cacgcggatg gtggtgatgg ggcagagcaa tgcgctggcg
     3541 gtcatggctt tcgccccacg gggcagcctg ttacatgcgc ccgatatgta tatgaaaaag
     3601 ctggttgtta atcgactggc tgccggggcg atagatctgt ctttgcctct ggcggacaac
     3661 ttgcgtaacg tggcgcgggc gttaggtaaa ccgctggaca agctgcgtat ggttacgctg
     3721 gataaaccgc gcctgagtgc cgcaattgag gaagcaacac agctgggcgt gaaggttttt
     3781 gccctgccgg atggcgatgt cgccgccagc gtgctgacct gctggcagga taatccttac
     3841 gatgtgatgt acaccatcgg cggtgcgcca gaaggcgtga tttctgcctg tgccgttaaa
     3901 gcattaggcg gggatatgca ggccgagctg attgactctt gtcaggctaa aggggattac
     3961 acggaaaatc ggcagattgc agagcaggag cgcaagcgtt gcaaggaaat gggcgttgac
     4021 gttaaccgtg tgtactcgct cgatgaactg gtgaggggga acgatatcct ctttagcgct
     4081 acgggcgtga cgggcggcga gttggtgaac ggaatccaac agacggcgaa tggggtgcgg
     4141 acgcagacat tactgatcgg cggcgcggac caaacgtgta atataataga ctccctgcat
     4201 tgatggtgat ctatcggaag accaatggcg acgctgacag aagatgatgt gcttgagcaa
     4261 ctggatgcac aggacaattt attttcattt atgaaaacta cgcattctat tttgctccag
     4321 gggatacgcc agtttctgcc gtcgctgttt gtcgataatg atgaagagat cgtcgaatat
     4381 gcagtgaagc cgttactcgc ccaaagcggc ccgcttgacg atattgatgt tgcactgcgt
     4441 ttgatttatg cgctggggaa aatggataaa tggctgtacg ccgatatcac gcatttttcc
     4501 cagttctggc attacctgaa cgaacaggat gaaatgcccg gatttgccga tgacatgacc
     4561 tgggatttta tcagcaacgt caatagcata acccgcaatg cgacgctcta tgacgcgttg
     4621 aaagcgatga agttcgccga tttcgccgtc tggtcagagg cgcgttttag caggatggtc
     4681 aaaacggcgc tgacgctggc agtaacgacc accttaaagg aattaacgcc gtgaaaattg
     4741 aattaacggt gaatgggctg aagatacagg cacagtacca gaacgaagag attgaaaatg
     4801 tacataaacc gttgctgcac atgttggcgg ccttacagac ggtaaatccg cagcggcgta
     4861 cggtggtttt tctttgcgct ccgccgggga cgggtaaatc taccctaacc accttctggg
     4921 aatatctcgc gcaacaagac ccggagctgc ccgctattca gacgctgccg atggatggtt
     4981 ttcatcatta caatagctgg ctggatgcgc atcaattgcg ccccttcaaa ggcgcaccag
     5041 agacatttga cgttgcgaaa ctggcggaaa atctgcgcca ggtaatggaa ggggattgta
     5101 cgtggccgca gtacgatcga caaaagcatg atcctgttga agatgcgttg cacgttaccg
     5161 caccactcgt catcgtcgaa ggaaactggc tattattgga tgatgaaaag tggcaggagt
     5221 tggcatcttt ctgcgatttt tctattttta ttcatgcacc tgctcaaatt ctgcgggagc
     5281 gcttgattag tcgtaaaatt gcgggaggat tatcgcggca agaagccgaa gctttttatg
     5341 cccgaaccga tggtcccaat gtcgagcgtg tgctggtgaa tagtcgtcag gccaatctga
     5401 tattagagat gaccgaagaa ggcagatatc acttcaaaag ctgacaattt attccagtta
     5461 ttgcaggggt attattacgc cagcctggaa taaagctccc tacaaaaggg gataaaagtg
     5521 tgatgtaagt cagataaatg tcttcttcgg ctggacaatc attcctttta ttccacgttt
     5581 cgcttatcct agctgaagcg tttcagtcga ttaaatgttc gacaattaac caatcagtcg
     5641 cagtttgcga caggtaaggt ttccccggac gatttgctgg attactctgt ctggcaaatt
     5701 tgctcaggga aaaccttgca ggagatctat gaccgtacgc gtagcgataa atggcttcgg
     5761 tcgcatcggg cgtaatgtgg ttcgtgcttt gtatgaatcc ggacgccggg cggaaattac
     5821 cgtggtggca atcaacgaac tggcggatgc tgcgggcatg gcgcatttgt tgaaatatga
     5881 caccagccat ggccgttttg catgggaagt acgacaggaa cgcgatcaac tttttgttgg
     5941 tgatgacgcc atccgtgtat tgcatgaacg ttcactgcaa tcgctcccct ggcgtgaact
     6001 tggcgttgat gtagtcctcg actgcaccgg cgtatatggc tcccgcgagc atggcgaagc
     6061 gcatattgcc gccggggcca aaaaagtgct cttttcacat cctggcagta acgatctcga
     6121 cgcgaccgtt gtttacggcg tcaatcagga tcaacttcgt gcggaacacc gcatcgtttc
     6181 taacgcttcc tgtaccacga attgcataat tcccgtcatc aaattgttag atgatgcgta
     6241 cggtattgag tccggcactg tgaccacaat tcactccgcc atgcacgatc aacaggttat
     6301 tgatgcatac catcctgacc tgcgtcgcac ccgggcagcc agccagtcga tcattccggt
     6361 cgatactaaa ctggccgccg gtatcacacg attttttccg caatttaacg atcgctttga
     6421 agcgattgcg gtacgtgtgc caaccataaa tgtgacggca atcgatttaa gcgtgacggt
     6481 gaagaaacct gtaaaagcca atgaagtcaa cctgttgctg caaaaagcag cacaaggtgc
     6541 atttcatggt atagttgact atacggaatt gccgttggtc tctgtagatt ttaaccacga
     6601 tccgcacagt gccattgtcg atggcaccca aacccgggtt agtggcgcac acctgatcaa
     6661 aacgttggtc tggtgcgata acgaatgggg ctttgctaac cgaatgctcg acacgacgtt
     6721 agctatggct actgttgctt tcaggtaaga cgcaagcagc gtctgcaaaa cttttagaat
     6781 caacgagagg attcaccatg tctgtaatta agatgaccga tctggatctt gctgggaaac
     6841 gtgtatttat ccgtgcggat ctgaacgtac cagtaaaaga cgggaaagta accagcgacg
     6901 cgcgtatccg tgcttctctg ccgaccatcg aacttgccct gaaacaaggc gcaaaagtga
     6961 tggtaacttc ccacctgggt cgtcctaccg aaggcgagta caacgaagaa ttctctctgc
     7021 tgccggttgt taactacctg aaagacaaac tgtctaaccc ggttcgtctg gttaaagatt
     7081 acctcgacgg cgttgacgtt gctgaaggtg aactggttgt tctggaaaac gttcgcttca
     7141 acaaaggcga gaagaaagac gacgaaaccc tgtccaaaaa atacgctgca ctgtgtgacg
     7201 tgttcgtaat ggacgcattc ggtactgctc accgcgcgca ggcttctact cacggtatcg
     7261 gtaaattcgc tgacgttgcg tgcgcaggcc cgctgctggc agctgaactg gacgcgctgg
     7321 gtaaagcact gaaagaacct gctcgcccga tggtggctat cgttggtggt tctaaagtat
     7381 ctaccaaact gaccgttctg gactccctgt ctaaaatcgc tgaccagctg attgttggtg
     7441 gtggtatcgc taacaccttt atcgcggcac aaggccacga tgtgggtaaa tccctgtacg
     7501 aagctgatct ggttgacgaa gcaaaacgtc tgctgaccac ctgcaacatc ccggttccgt
     7561 ctgatgttcg tgtagcaact gagttctctg aaactgcacc ggctaccctg aaatctgtta
     7621 acgatgtgaa agctgacgag cagatcctgg atatcggtga tgcttccgct caggaactgg
     7681 ctgaaatcct gaagaatgcg aaaaccattt tgtggaacgg tccggttggc gtgttcgaat
     7741 tcccgaactt ccgcaaaggt actgaaatcg tggctaacgc tatcgcagac agcgaagcgt
     7801 tctccatcgc tggcggcggc gacactctgg cagcaatcga cctgttcggc attgctgaca
     7861 aaatctccta catctccact ggcggcggcg cattcctcga attcgtggaa ggtaaagtac
     7921 tgcctgcagt agcgatgctc gaagagcgcg ctaagaagta aaaaatcaca gggcagggaa
     7981 acctgccctt gttgcagcgc gcttttaaag cacgcacttt ttcatactct aaataattcg
     8041 agttgcagga aggcgacaag agagtgaatc ccatgagctt acataagtaa gtgactgggg
     8101 tgagcgaacg cagacgcagc acatgcaact tgaagtatga cgagtataag gcccgacgat
     8161 acaggacaag agacatgtct aagatttttg atttcgtaaa acctggcgta atcactggtg
     8221 atgacgtaca gaaagttttc caggtagcaa aagaaaacaa cttcgcactg ccagcagtaa
     8281 actgcgtcgg tactgactcc atcaacgccg tgctggaaac cgctgctaaa gttaaagcgc
     8341 cggttatcgt tcagttctcc aacggtggtg cttcctttat cgctggtaaa ggcgtgaaat
     8401 ctgacgttcc gcagggtgct gctatcctgg gcgcgatctc tggtgcgcat cacgttcacc
     8461 agatggctga acattatggt gttccggtta tcctgcacac cgaccactgc gcgaagaaac
     8521 tgctgccgtg gatcgacggt ctgctggacg cgggtgaaaa acacttcgca gctaccggca
     8581 agccgctgtt ctcttctcac atgatcgacc tgtcagaaga atctctgcaa gagaacatcg
     8641 aaatctgctc taaatacctg gagcgcatgt ccaaaatcgg catgactctg gaaatcgaac
     8701 tgggttgcac cggtggtgaa gaagacggcg tggacaacag ccacatggac gcttctgcac
     8761 tgtacaccca gccggaagac gttgattatg cgtataccga gctgagcaaa atcagcccgc
     8821 gtttcaccat cgcagcgtcc ttcggtaacg tacacggtgt ttacaagccg ggtaacgttg
     8881 ttctgactcc gactatcctg cgcgactctc aggaatatgt ttctaagaaa cacaacctgc
     8941 cgcacaacag cctgaacttc gtattccacg gtggttccgg ttctactgct caggaaatca
     9001 aagactccgt aagctacggc gtagtgaaaa tgaacattga taccgatacc caatgggcaa
     9061 cctgggaagg cgttctgaac tactacaaag cgaacgaagc ttacctgcag ggtcagctgg
     9121 gtaacccgaa aggcgaagat cagccgaaca agaaatacta cgatccgcgc gtatggctgc
     9181 gtgcaggtca gacttctatg atcgctcgtc tggaaaaagc attccaggaa ctgaacgcga
     9241 tcgacgttct gtaagtcatt cctttctgct tatctcaagg cccgctctgc gggtcttttt
     9301 ttcgccaaaa gcaaggtgat gcagatgaga aatctgtgat ctatttggca aaattatgct
     9361 ttattgttta cccttgtcag actgcccgtc ataaggcggc ggagtgtatt tctccatttt
     9421 gagtcagttg aaaaggaata ttgaatggaa gatttgaatg ttgtcgatag cataaacggc
     9481 gcgggaagct ggctggtggc taaccaggcg ctgctgctaa gttatgcagt aaacatcgtg
     9541 gcggcactcg cgatcatcat cgttggtttg attatcgcgc ggatgatttc caacgcggtg
     9601 aatcgcctga tgatctcccg taaaatcgat gccactgttg ctgattttct ctctgcatta
     9661 gtccgttacg gtattatcgc ctttacgcta atcgctgcac tggggcgcgt gggcgtacaa
     9721 acagcgtcag tcattgctgt actcggtgcc gcaggcttag cggttggtct ggctttgcag
     9781 gggtcactgt ctaacctggc cgctggcgtg ttacttgtta tgttccgccc gttccgtgcc
     9841 ggagaatatg ttgatctcgg cggcgtagcc ggtactgtgc tgagtgtgca gattttctcc
     9901 accaccatgc gtactgcaga cggtaaaatt atcgttattc cgaacggtaa aattattgcc
     9961 ggaaatatta ttaacttctc tcgcgagcca gttcgccgta acgaatttat tattggcgtg
    10021 gcgtatgatt ccgatatcga tcaggttaag cagatcctga ccgatattat ccagtctgaa
    10081 gatcgcattt tgaaagatcg cgaaatgact gtgcgcctga acgaacttgg tgcatcgtcg
    10141 attaatttcg tggtccgcgt ctggagcaac agcggcgatc tgcaaaacgt gtactgggat
    10201 gtgctggagc gtattaaacg tgaatttgat gccgccggta tcagcttccc gtacccgcaa
    10261 atggatgtga actttaagcg ggtgaaagaa gacaaagctg cgtaatcaac gctgaggcca
    10321 gataatactc tctggcctct ctcttattag tttttctgat tgccaattaa tattatcaat
    10381 ttccgctaat aacaatcccg cgatatagtc tctgcatcag atacttaatt cggaatatcc
    10441 aacgtgtttt cttattactt tcaaggtctt gcacttgggg cggctatgat cctaccgctc
    10501 ggtccacaaa atgcttttgt gatgaatcag ggcattcgtc gtcagtacca cattatgatt
    10561 gccttacttt gcgctatcag cgatttggtc ctgatttgcg ccgggatttt tggtggcagc
    10621 gcgttattga tgcagtcgcc gtggttgctg gcgctggtca cctggggcgg cgtagccttc
    10681 ttgctgtggt atggttttgg cgcttttaaa acagcgatga gcagtaatat tgagttagcc
    10741 agtgccgaag tcctgaagca aggcagatgg aaaattatcg ccaccatgct ggcagtgacc
    10801 tggctgaatc cgcatgttta cctggatact tttgttgtac tgggcagtct tggcgggcaa
    10861 cttgatgtgg aaccaaaacg ttggtttgcg ctcgggacaa ttagcgcctc tttcctgtgg
    10921 ttctttggtc tggctattct cgcagcctgg ctggcaccgc gcctgcgcac ggcaaaatca
    10981 cagcgcatta tcaatctggt agtgggatgt gttatgtggt ttattgcctt gcagctggcg
    11041 agagacggta ttgttcatgc acaagccttg ctcagttagg cgctatctga tggaaaaata
    11101 aaacagaggc gctaagcttg cctccagagg tcctgaattt taggcaagtc aattaacacg
    11161 gagagactaa cgtgaagttc aaagttatcg ccctggcggc attaatgggt attagcggga
    11221 tggcagcgca ggctaacgaa ttgccggatg gaccgcatat tgtcacctcc ggtacggcaa
    11281 gcgtggatgc ggtgccagac attgccactc ttgcgattga agtcaacgtg gccgcgaagg
    11341 atgccgctac tgccaagaaa caggcggatg agcgcgtcgc acaatacatt tccttccttg
    11401 aactcaatca gatcgcgaaa aaagatatca gctcagcgaa cttacgcacc cagccagatt
    11461 atgattatca ggatggtaaa agtatcctga aaggctaccg tgctgtgaga acggtggaag
    11521 tcacgctccg tcagttagac aaactgaatt ccttgctgga tggcgcgctg aaggcgggtc
    11581 ttaacgaaat tcgttctgta tcgctgggcg tggcgcagcc ggatgcctat aaagacaaag
    11641 cgcgtaaggc agcgattgat aacgcgattc atcaggcgca ggaactggcg aacggctttc
    11701 atcgtaaact ggggccggta tatagtgtgc gctaccatgt ttccaactat cagcccagcc
    11761 caatggtgcg gatgatgaaa gccgatgccg cgccggtgtc cgcccaggaa acttacgagc
    11821 aggccgctat tcagtttgat gatcaggtcg atgtggtctt ccagttagaa cctgtggatc
    11881 aacaacccgc taaaacacct gcagcacaat aatatttgta gcccgacgta ttcgtcgggc
    11941 taatgccttt acccgatatt gcctcctttt tctgtaattt tctgtaaaac catgacgtaa
    12001 tcacctatct ttgctcgcgg tttcatcaag gataggctga ctgaaacggt taagcggaca
    12061 aggatgcggg ggagggggta tggacatttt tatttcgaaa aagatgcgca atttcatttt
    12121 attagcgcaa accaataata ttgcccgggc ggcagaaaaa atccatatga cagcttcgcc
    12181 atttggtaaa agcattgccg cgctggaaga acaaattggc tatacgctat ttacccgcaa
    12241 agataataat atcagcctca ataaagccgg acaggaactg tatcaaaaac tgttcccggt
    12301 ttatcaacgg ctttctgcga ttgataatga aatccataac tcagggcgtc gttcacgaga
    12361 aattgtgatt ggcatagata atacttatcc gacaattatt ttcgatcagt tgattagtct
    12421 cggagataag tatgaaggcg tgaccgccca gccagttgag ttcagcgaaa atggcgttat
    12481 cgataatctt tttgaccgtc agttagattt tattatctcc ccacagcatg tatctgcacg
    12541 agttcaggaa ctggaaaacc tgactatcag tgaactaccg ccgctgcgat tgggctttct
    12601 cgtttctcgt cgttatgaag agcgacaaga gcaagagttg cttcaggagc taccctggct
    12661 acaaatgcgc tttcagaacc gggctaactt cgaagcaatg attgatgcga atatgcgtcc
    12721 ttgtgggatc aatcccacca ttatttatcg tccctacagt tttatggcca aaatcagcgc
    12781 cgtggagcgc ggacattttc tgacggtgat cccgcatttt gcctggcgac tggtgaatcc
    12841 ggcaacgctg aaatattttg atgcgccgca caggccgatg tatatgcagg aatatcttta
    12901 ttcaatcaga aatcatcgtt ataccgccac gatgcttcag catattgctg aagatcgtga
    12961 cgggacgagt cattaaccca gcatcgagcc ggttgcaatt aaattacggt gtaagtcgaa
    13021 gacgtggcta agatcgtggt gaatatgtcc gccgggcgca ttttccagat agcgatgcag
    13081 ataatcccga tacataggat gtgcacaatt atcaatgata gtgcgggcgc gttgaagcgg
    13141 ggaaagacca cgcagatcgg cgatgccttg ttcggtgatg atcactttga cgctgtgttc
    13201 gctgtgatca acatggctgc acattggcac gacggttgag atcttgcctt ctttagcaat
    13261 cgacggggcc ataaagatcg acagatacgc gttgcgttca aaatcaccgc tgccaccgat
    13321 gccgttcatc agatcgaccc cggctacgtg cgttgagttg gcatgcccgt aaatatcaaa
    13381 ctccaggccg acgttcagag cgatgacgcc cagacgacgg atgatttccg ggttattgga
    13441 aatctcctgc ggacgcaaca caatgcggct ggcaaagtaa tccatattgt cgtaaatctt
    13501 gcgcagggaa tcggccgaga tagtcaggct ggaggcgctg gccccgctga ttttgcccgt
    13561 ttccagtaaa tgcaccaccg attcctgtag cacttccgaa tacatcataa acggaggaat
    13621 ttccggattt tcccccagac gcgccattac cgcattattg atgttgccta cgccactttg
    13681 cagcggcaga aattccggcg gaatacgccc atgcgccatt tcctgcaaca agaacgtgac
    13741 cacgttatcg gcaatctgct ggcacatggg attttgctta tccagcatat taccggcgtc
    13801 aggcaagttg gtttccacga cggcgacaat ctttttcgga tcgatttgca catagcgggt
    13861 accgacgcga tccattgcat ggaagatcga cacgctattg cgccgcggtg gcgcgccagg
    13921 aatcacaata tctgccagtt ctgcaacgcg cggatcgtga tagtgattga gttcaatgat
    13981 cactttcttc gcccgcagca gccaggtcgg cgcattaccg atcccgctgg ttaaccagac
    14041 tcgaccatcc ggtgccagtg ccgatgcttc aatgacggca acatcaatgt cgccgaagaa
    14101 accgtaattg accatttgcg ccacttcgct caaatgcagg tcaacgaaac tcaccgcgcc
    14161 ctgattgatc tttttacgta aaccggacga tgtttgatat ggcgcacgcc aggaaacagc
    14221 atcggcgtca gaaagtacat cgtcagcggc ggcgctgatt gacgcccccg tcagcaggcg
    14281 aatttgatac ggctttttgg cctcatgctg ttcgttagct ctgcgggcaa tcgcgttggg
    14341 taaggctttc ggcgaacccg ctggggtaaa gccgctaaat gccaccatgt cgttatgctg
    14401 gataatttct gccgcttcat cggcggtcat ccttgtccac tgagtttcca ttttaactcc
    14461 ttcgttcgca gggattaatg accaacgaaa ttaggtttac gtttttcgag gaaagcgttc
    14521 atcccttcct ggtaatcttc gctgtcataa accgcgcggc gcatcccctg aatacgttca
    14581 aattcatcgg agttcatggt gtgcgcttcg cccagtacac gcagctcttc tttgataacg
    14641 gcaatggcta acggcgcttt ctcagagatg tggtgcgcca tttgtaaggt gaaatcttcc
    14701 agttcttcca cttccacaac atggttgagg atgccgacag ccagcgcgcg ctgggctgtg
    14761 attggcgaag cggtaaaaat cagctctttg acaatgtgga agtccgcgtc gcgggtcagg
    14821 ttgtgaatgc cgaccaggtt atacgggacg ccgaggttta caggtgtcat tgagaaggtt
    14881 gaggtactgg cggcgatgat cagatcggaa ctcatgatca tttcaaatgc gccaccccag
    14941 acactacctt ccaccatcga aatgatcggt ttcgggaatt tttggatcat gcgggtgatt
    15001 tgacgcaacg gatcatcata ggagagcgga tcgcgaccgc cagacggcag ttcgtgaata
    15061 tcgtgacctg cggagaagac tttggaacca ctcggcgcgc gcaaaatgat acagcgaatt
    15121 tccggccggt tgagatcgct taatgcctgc ataagatcat caataaagac tttacttaag
    15181 gcattaagtt tgcggccata gttaaactca atgaccgcca ctttgttgat agtgacaacg
    15241 ttcacatact gataagacat aaaaattcct ttaatcaaaa tattgcgtct ggataaattc
    15301 actgagctgc cgcaggccgg tgcgcggtga gagcgtattg tttttgactg ctaaaagcgt
    15361 ctggcggtaa tagcgatcga aatcttcatt cgcgaacagg cgattcagta cttcttcttc
    15421 ggtctgctta cgcagccatt ccaccgattg ttgttgccgc acttgttgta atcgaccact
    15481 ggcagttagc gcggttttga agtcgatgat ggcgtgccag atctcatcga ttccacgttt
    15541 ttccagtgcg ctacaagtca gaacccgtgg ctgccattcg tcgtatttac gtcgcagaat
    15601 atgcagggca ctctcgtaca tatgccgggc aatggcgaca ttggtatggt tatcgccatc
    15661 gtctttgttg ataacgatca gatctgccac ttccatcagc ccctttttaa tgccctgcag
    15721 atcatcgccg ccaccggcaa tttgcaacga gataaaacag tccaccatgc gggcgacttc
    15781 cgtttctgac tgcccgacgc caaccgtttc gacaatcact acgtcataac ccgccgcttc
    15841 gcataacagc attaattccc gcgctcgctg actggcaccg cccagatggc cggaggaggg
    15901 taccgggcga ataaacgccg cctcggcacg cgccaggtca ttcatgcggg ttttatcccc
    15961 gagaatgcta ccgccagtga ccgggctgct gggatcgacc gcaataacag caacctttaa
    16021 tccctcacga atcaacaaca tgccaaaggc ttcaagaaag gtacttttcc ccgcgccggg
    16081 tgtgccggta acgcccagtc gcagggcgtt accacagaac ggcataatgg cgtcaagcag
    16141 ctgagtactt agcgcctgat gacgcgggtg acggctttcc accaacgtca tggcctgggc
    16201 gagcgtggca cgctcaccat gacgtaagcg gcgaatactt tctgccagcg tggcttcatt
    16261 aatcatgatg ctggcttatc agattcagta cgtcgcgcac gctatcgagc ataggtgtac
    16321 ctggaccata aatcgccgcc acgccgcgct cttgcaggaa ggcgtaatcc tgcggcggaa
    16381 tgacgccacc cgcgaccacg cagatatctt tgcgtcccca ttttttcagc gcttcgacca
    16441 gttctgggat cagcgtttta tgaccggcag ccagtgagga tgcgcccact acgtgaacgt
    16501 cgttttctac ggccaggcgg gcgatctctt caggtgtaga gaacatcggg cttaaatcta
    16561 cgtcgaaacc gagatcggaa taggcgctgg cgatcacttt cgcgccgcga tcgtgcccat
    16621 cctggcccat tttggcgatc agaatgcgcg ggcgacgacc attgtcggca aggaactgct
    16681 ccgtttgcgc aacaatggca tcgaactcgg cggccgattt ctcagactga tgatagcttt
    16741 gcgcaatcac gccggtaaca cactggcttg gcaccagata acggtcgaaa gcggcttcca
    16801 gcgcatcgga aatttcaccc agggtggcgc gaacgcgagc agcattaaca gcggcagcca
    16861 gcaggttttc gttatgctgt gcggcgtgag tcagggcgtt caacgcggcg gttacggcgg
    16921 catcatcacg ggtggcgcga atgcgttcca gcgaagcaat ttgctcgtta cgcaccatca
    16981 cgttgtcgat ctcaagtaca tcggtttcgt cttcgtgatc cagtttgtac ttgttgacac
    17041 caacgatgac acgcttgccc tggtcgatca gcgactgttc gcgcgctgag gcctcttcga
    17101 tcattcgttt tggcagacct gcttcgatcg ctttcgccat gccaccggct tcgtcgatct
    17161 gttggataat agctctggct tgtttgacga tttgatcggt cagcgactca atgtaatagg
    17221 atccggccag tggatcgacg gtgcggcaga gttctgattc ttcctgaatg atgatctggg
    17281 tattgcgggc aatgcgtgct gagaaatcgg taggcaaacc aagcgcttca tcaaaggcgt
    17341 tggtatgcag tgactgagta ccgcccagcg ttgcagccag tgcttcaatg gtggtgcgga
    17401 taacgttgtt atacggatcc tgttcagtca ggctccagcc tgaggtctgg cagtgggtac
    17461 gcagcgccag tgatttcgga tcctgtgcgc caaatccact gaccgcttcg ctccataaat
    17521 aacgtgccgc acgcaacatg gcgacgttca taaacagatc catgccaatg ccgaagaaga
    17581 acgacaggcg aggagcgaag tcatcaattt tcagcccggc ggagattgct gctttgatgt
    17641 actcaatccc atcagcgagc gtaaatgcta cctgctgcac gcagttggca cccgcttcac
    17701 ccatatggta accgctgata ctgatggtat taaatcgcgg catgttgccg gaacaccagg
    17761 cgatgatgtc ggcgataata cgcattgacg gttttggcag gtaaatatag gtgttgcggc
    17821 aaagatactc tttgaggata tcgttttgaa tggtgccggt cagtttatcg ggtgtaacgc
    17881 cttgctcttc tgcggcgaca atataaaacg ccagtactgg tagcactgcg ccattcatgg
    17941 tcatcgaaac cgacatttta tccagcggga tctggtcgaa cagaactttc atatcttcca
    18001 cggtgtcgat agcgacgccc gctttgccga cgtcgcccgc cacgcgtggg ttatcggagt
    18061 cgtagccacg gtgggtggca aggtcaaacg caacggaaag acctttttgc ccggcggcca
    18121 ggttacggcg ataaaaagcg ttggactctt ttgctgttga aaaaccagca tactgacgga
    18181 tggtccacgg ttgggcggta tacatagtgg cacgcgggcc acgaacgtag ggcggcaaac
    18241 caggaagggt acctgtcacc tccagattat cgagatcggc ttcggtatac agcggcttga
    18301 tggcgatccc ttccgcggtt tgctgaacca gcgagtcgac agttttctcc cgacggctca
    18361 attccttgtt ggcaagctgt tgccactcct gcaccttaga cattatttgc tccgtaaaac
    18421 gcaatcaaaa tgagggtatt tgcgcttacg gtgagctttt tacgtgggtt tgtcgccagc
    18481 aaaaaaaatg cattcagacg tcgttttttg ccgcctgaga tttgtctatt ttgtgatgtg
    18541 attcaaggaa tacaaaaaga agtgaggata acgcctgata tgtgctatca ggcattattt
    18601 gatggattaa tcctgacgaa ggactttgtg accataatca agcaacgcat cagtgacttt
    18661 acgcatcatg cggctttcag gagcaaagcg gtgccagtag agcatccgtc gttgaaatag
    18721 cccaggcgtt aagtcaatca gttcaccgct ggccagctct ttctcgattt gcaggtgcgg
    18781 gatcatacag caggtggtgc cctggcgagc aagttgcacg aacgcttctg aagaattaac
    18841 gatatggcag ggtacgctgc caggaggcag atcgaagttt tgctgcaaaa aggcctggtg
    18901 catatcgtca agatggtcaa aggcgaccac aggtgctttc aataatgccg aacgcgttac
    18961 gccgttaggg aaatattttt cggcaaacgg ttttgagctg acgaacagat agtcgagcgc
    19021 accgagctta tcgacaagac agctcggcag cgcctgatgt tgaatactca ccgcgccgac
    19081 cacttcgccg cggcgcagac gttcctgagt gcgggtttca tcttctacct gcaagttgag
    19141 gcggataggc gaatcagcta acacaggagc cagcgccgga agcaaccacg tcgccagact
    19201 gtcagcgttg accgccagtg aaagcagcag cggagttgaa ccggtttgtt catcgcccag
    19261 ccactcttct tccagtaact ccacctggcg tagcagtgcc agcagttttt gcccttgttc
    19321 tgtcgggcgc ggtggtacgg tacgcaccaa cagcggctgc ccgaacatat tttccagttg
    19381 cttaatgcgc tgtgagacgg ctgattgtgt aatgcacagc ttttgtgccg cgcgctcaaa
    19441 tcctcgttca cgtatcaccg catccagtgc ctgtaatgtt ctgtagtccg ggcgtttcat
    19501 tgctgttgtc tgctcctggt aaatgctttt tcctgcacta tgacacaatt ttgtgtcagg
    19561 ttgcatatac cgaagtcgtt ggcgggtaac atttgtatca gtggttaacg cgcagcttaa
    19621 aagatcaggg atttgcgcta atgcatttcg ctcagcgagt tcgcgccctg gttgtgttga
    19681 atggcgtggc gttattgcct ctgttcgcat cgtggagcgg catacccaga ccgttgtatg
    19741 ctttatttgc ggggcgaaag gggatgcccg ccattgcgcg atattttatg gatgagttaa
    19801 ccacccggct tgccaacggg gtctgaatcg ctttttttgt atataatgcg tgtgaaattt
    19861 cataccacag gcgaaacgat catgacgcag gatgaattga aaaaagcagt aggatgggcg
    19921 gcacttcagt atgttcagcc cggcaccatt gttggtgtag gtacaggttc caccgccgca
    19981 cactttattg acgcgctcgg cacgatgaaa ggccagattg aaggggccgt ttccagttca
    20041 gatgcttcca ctgaaaaact gaaaagcctc ggcattcacg tttttgatct caacgaagtc
    20101 gacagccttg gcatctacgt tgatggcgca gatgaaatca acggccacat gcagatgatc
    20161 aaaggcggcg gcgcggcgct gacccgtgaa aaaatcattg cctcggttgc ggaaaaattt
    20221 atctgtattg cagacgcatc caagcaggtt gatattctgg gcaaattccc gctgccagta
    20281 gaagttatcc cgatggcacg tagtgcagtg gcgcgccagc tggtgaaact gggcggtcgt
    20341 ccggaatacc gccagggcgt ggtgaccgat aacggcaacg tgatcctcga cgtccacggc
    20401 atggaaatcc ttgacccgat agcgatggaa aacgccataa atgcgattcc tggcgtggtg
    20461 actgttggct tgtttgctaa ccgtggcgcg gacgttgcgc tgattggcac acctgacggt
    20521 gtcaaaacaa ttgtgaaatg atctgacggg ggaacctccc ccgttaaaaa aattctcttc
    20581 attaaatttg gtgacatgtg tcacgttttt acctggctat tgtcgattgc tctaaataaa
    20641 ccctctaaac cagcatattc atccaagaaa tacctttgcg tgatatttcc tcaacatcgc
    20701 gacgcaaacg ttcatattgc cgcaatatta ttttttgata tgttgaaagg cggatgcaaa
    20761 tccgcacaca acatttcaaa agacaggatt gggtaaatgg caaaggtatc gctggagaaa
    20821 gacaagatta agtttctgct ggtagaaggc gtgcaccaaa aggcgctgga aagccttcgt
    20881 gcagcgggtt acaccaacat cgaatttcac aaaggcgcgc tggatgatga acaattaaaa
    20941 gaatccatcc gcgatgccca cttcatcggc ctgcgatccc gtacccatct gactgaagac
    21001 gtgatcaacg ccgcagaaaa actggtcgct attggctgtt tctgtatcgg aacaaaccag
    21061 gttgatctgg atgcggcggc aaaacgcggg atcccggtat ttaacgcacc gttctcaaat
    21121 acgcgctctg ttgcggagct ggtgattggc gaactgctgc tgctattgcg cggcgtgccg
    21181 gaagccaatg ctaaagcgca tcgtggcgtg tggaacaaac tggcggcggg ttcttttgaa
    21241 gcgcgcggca aaaagctggg tatcatcggc tacggtcata ttggtacgca attgggcatt
    21301 ctggctgaat cgctgggaat gtatgtttac ttttatgata ttgaaaacaa actgccgctg
    21361 ggcaacgcca ctcaggtaca gcatctttct gacctgctga atatgagcga tgtggtgagt
    21421 ctgcatgtac cagagaatcc gtccaccaaa aatatgatgg gcgcgaaaga gatttcgcta
    21481 atgaaacccg gctcgctgct gattaatgct tcgcgcggta ctgtggtgga tattccagcg
    21541 ctgtgtgacg cgctggcgag caaacatctg gcgggggcgg caatcgacgt attcccgact
    21601 gaaccggcga ccaatagcga tccatttacc tctccgctgt gtgaatttga caacgtcctt
    21661 ctgacgccac acattggcgg ttcgactcag gaagcgcagg agaatatcgg cttggaagtt
    21721 gcgggtaaat tgatcaagta ttctgacaat ggctcaacgc tctctgcggt gaacttcccg
    21781 gaagtctcgc tgccactgca cggtgggcgt cgtctgatgc acatccacga aaaccgtccg
    21841 ggcgtgctaa ctgcgctcaa caaaattttt gccgagcagg gcgtcaacat cgccgcgcaa
    21901 tatctacaaa cttccgccca gatgggttat gtagttattg atattgaagc cgacgaagac
    21961 gttgccgaaa aagcgctgca ggcaatgaaa gctattccgg gtaccattcg cgcccgtctg
    22021 ctgtactaat tccccttctc tgaaaattaa cgggcaggtc actgacttgc ccgttttttt
    22081 atcccttctc cacaccgttg ccgcattgcg cattctactt tgcgagtctg ctccagaaaa
    22141 taaatcaaag tcagatcaat gcgttgtgtt tatttaatgg ggatttacga tggcagggca
    22201 gcatggggct gtaacggtaa agccctcacc gaagcgaggg cttgaaggag aagggttatg
    22261 atgcgacttg tcatcatact gattgtactg ttactcataa gtttcccagc ttattaacag
    22321 tcagtctcag gggaggagtc atcctccctt acccttactc actaaattag gttaaagaat
    22381 caacgatgtc aatcagggcg atgcaggtgt gtcgccctca ccactcccag actttcgacg
    22441 gtgtaaccac cgcaggaaga gggatatccc actcttcaac ggggagtttt tccactaact
    22501 gacaatcatg cgcataaccc accggttgca ttttatagtg ctgccagttt tgtaaggtcc
    22561 gatcataaaa accaccgccc attcccaggc gctgaccgta ctcatcaaag gcgaccagcg
    22621 gtgtgatcaa cacgtctaat cgggaaaggg gtagcacgtc acgcacatcc agttttggct
    22681 catggatctt caacctgttc atcaccagtt cgctttgcgg atggtaattc aggaacagca
    22741 aattaccggc actgaaggga tgcaaaactg gaagatatac gcgcttaccg gcgcgccaga
    22801 gttgttctat gagtggcagg gtgtcgagtt cgccatcaaa agagaggaat acagcgaccg
    22861 tatgtgccat caccaccggg ggataagtca tcatccgggt agcggcttgt tgacccattt
    22921 cctgctgttg ttccggcgtt aacgcacgac gacgttgccg aatcattttg cggatttctt
    22981 gtcgggataa tgtcagtggg agttctggta gttgcgtcat ggctggtgcc agataagaag
    23041 ggaatctccg agatgccgcc gcaggctgta acccttgaac ccttggttca aggtgaatgt
    23101 gtcgtcgcag ttttaaggct tctcggacgg accgagcatg ctcaccaacc gcggagcgcc
    23161 acattcttgt ggtatgaaat atcggctcag gggactggcc cgcttgcgaa catctcagag
    23221 aaattttgtc ttcacggtta ctctaccaca gtaaaccgaa aagtgtcatt caaagttttg
    23281 gttagttttt tcggtgatgc gaccttgttc aagtaacgct tgttctatgg tctgctgcag
    23341 catccgaata cgctgttcca tacttgccgc gtagtcacga gtcttcgctt tttcttgcgc
    23401 taactcatag ctgatattca atgcggcaat gaagaccaac tgttcagtat ttgtgactct
    23461 agtgcgttct ttcagatctt gcaaccgttg gttcagatcg tccgctgcct gattcaacgc
    23521 atccctttgg tcaggcgggc agttcacacg cagtgaacgg ccaaaaattt ggatatcgac
    23581 gggttgtgca gacatgccac cttcctgctg attgactgcg ctgccttcgt ctttagaccc
    23641 atggtctgcg aaggggcgac actatagcta ccctgatgag aagagacaag cccttttctg
    23701 gtccaccagg gcccaaagtg gtagcatatc atgaatattc ctccctttga cgacgaatgc
    23761 ttatgtctat acagaacgaa atgcctggtt acaacgaaat gaaccagtat ctgaaccaac
    23821 aagggacggg tctgacccct gctgagatgc atggtttaat cagcgggatg atatgtggcg
    23881 gtaacgatga cagctcatgg ctgccgctac ttcacgacct gacgaacgaa ggcatggctt
    23941 tcggtcatga gctggcacag gcactgcgta aaatgcactc tgccaccagc gatgccctgc
    24001 aggatgacgg cttccttttt cagctttatc tgcctgatgg cgatgatgtc agcgttttcg
    24061 atcgggctga tgcgctggca ggttgggtca atcacttcct gcttggtctt ggcgttacgc
    24121 aaccgaagct ggacaaagtg accggagaaa ccggtgaagc catcgacgat ctgcgtaaca
    24181 tcgcgcagct gggctacgac gaagacgaag atcaggaaga gcttgaaatg tcgcttgaag
    24241 agatcatcga atacgttcgt gttgccgcgc tgttatgcca cgacaccttt actcatccgc
    24301 aaccgaccgc gccagaagta caaaaaccga ctctacacta aaaacaaaaa acgtaaggag
    24361 agtgttatga gtgagatatc ccggcaagag tttcagcgtc gccgtcaggc cctggtggag
    24421 caaatgcaac ccggcagcgc cgcgctgatt tttgctgcac cagaagtaac acgtagcgcc
    24481 gacagcgaat acccctatcg tcagaacagt gacttctggt acttcaccgg ctttaacgaa
    24541 ccggaagcgg tgctggtgct gattaaaagc gacgacactc ataaccacag cgttttgttt
    24601 aaccgcgttc gcgacctgac ggcagagatc tggtttggtc gtcgcttagg ccaggatgcc
    24661 gcgccagaga aactgggcgt tgaccgcgca ctggcattca gcgaaatcaa tcagcaactt
    24721 tatcaactgc ttaatggtct ggatgtggtt tatcacgctc agggcgaata tacctatgct
    24781 gatgaaatcg tgaacagcgc gctggaaaag ctgcgtaaag gctcgcgaca gaatctcact
    24841 gccccggcta cgatgatcga ctggcgtcct gttgttcatg aaatgcgcct gttcaaatca
    24901 ccagaagaga ttgccgtact tcgccgcgcg ggtgaaatca ccgcactggc gcatacccgg
    24961 gcgatggaaa aatgccgtcc ggggatgttc gagtaccatc tggaaggcga aattcaccac
    25021 gagtttaacc gccacggtgc gcgttaccct tcctataaca ccattgttgg cagcggtgaa
    25081 aacggctgca ttttgcacta caccgaaaac gagtgtgaac tgcgtgacgg tgatctggtg
    25141 ttgattgacg cgggttgtga atacaaaggt tacgctggcg acattacccg caccttcccg
    25201 gtcaacggca aattcactca ggctcagcgt gaaatctatg acattgtgct ggagtctctt
    25261 gaaaccagcc tgcgcctgta tcgtccggga acttccatgc aggaagtcac tggtgaagtg
    25321 gtgcgtatca tggttagcgg cctggtaaaa ctcggcatcc tgaagggtga tgttgatgaa
    25381 ctgatcgctc agaacgccca tcgtcctttc tttatgcatg gccttagcca ctggttagga
    25441 ctggatgtcc atgacgttgg cgtttatggt caggatcgct cgcgcattct ggaaccgggg
    25501 atggtactga ccgtagagcc tggtctgtat atcgcgccgg atgcggatgt gccagaacaa
    25561 tatcgcggta tcggcattcg tattgaagac gacattgtga ttaccgaaac cggcaacgaa
    25621 aacctcaccg ccagcgtggt gaaaaagccg gaagaaatcg aagcgttgat ggctgctgcg
    25681 agaaagcaat gagcgtaatc atcgtcggtg gcggcatggc gggcgcgacg ctggcgctgg
    25741 ctatttcccg gttaagtcac ggggcgctgc cggtacattt gattgaagcg actgcgccag
    25801 agtcacatgc tcatccgggc tttgatggac gagcgatagc gctggcggcg ggtacctgtc
    25861 agcaactggc gcgcatcggc gtctggcaat ctctggctga ttgcgcaacc gccatcacta
    25921 ccgtgcatgt cagcgatcgt ggtcatgccg gattcgtcac cctcgccgca gaagattacc
    25981 aactggcggc gctggggcag gttgtcgaat tgcataatgt cgggcaacgg ctgtttgcat
    26041 tgctgcgcaa agcacctggc gtaacgctgc attgtcctga tcgcgtggct aacgttgccc
    26101 gtactcagag tcacgttgaa gtgacgctgg agagtggcga gacgctgacg ggctgcgtgc
    26161 tggtagccgc tgatggcacc cattcagcgt tagccaccgt gtgcggcgtt gactggcagc
    26221 aggagcctta cgaacaactg gctgtgattg ccaacgttgc gacttccgtt gcgcatgaag
    26281 ggcgcgcttt tgaacgcttt acacaatatg gcccgctggc gatgttgccg atgtctgacg
    26341 gacgctgttc gctggtctgg tgtcatccac tggaacggcg cgaagaggta ctgtcttgga
    26401 gcgacgagaa gttttgccgt gaactccagt cggcctttgg ttggcgactt gggaaaatta
    26461 cccacgctgg taaacgcagt gcttatccgc tggcgttaac ccgcgccgcc agacctatta
    26521 cccatcgtac cgtgctggtg ggcaatgcgg cgcaaactct gcacccgatt gccgggcaag
    26581 ggttcaacct cggtatgcga gatgtcatga gccttgcgga aaccctgact caggcgcagg
    26641 agcgcggaga agacatgggt gattacggcg tattgtgccg ttatcagcag cgtcgacagc
    26701 gcgatcgcga agcaaccatc ggcgtcacgg acagccttgt acatcttttt gccaaccgtt
    26761 gggggccgct ggttgtcggg cgcaacatcg ggctgatggc aatggaatta ttcaccccag
    26821 cacgcgatgt gctggcgcag cgcaccctcg ggtgggtggc gcgttgaagg cggttttaag
    26881 gaataaacaa tgcaaagtgt tgatgtagcc attgttggcg gcggcatggt ggggctggcg
    26941 gttgcctgcg gcttgcaggg tagcggctta cgcgttgccg tactggagca gcgcgtaccg
    27001 gaacctctgg cggcggatgc accaccacaa ctgcgcgttt cggctatcaa tgccgccagc
    27061 gaaaaattac tcacccgtct tggcgtctgg caggacattc tctctcgtag ggccagttgt
    27121 tatcacggta tggaagtgtg ggacaaagac agttttggtc acatttcgtt tgacgatcaa
    27181 agcatgggct atagccatct tgggcatatc gttgaaaact cggtgattca ctacgcgctg
    27241 tggaacaaag cgcagcagtc gtcagatatc actctgttgg cccccgcaga attacagcag
    27301 gtcgcctggg gagaaaatga aaccttcctg acgttgaaag atggcagtat gttaacggcg
    27361 cgtctggtga ttggcgcgga cggcgctaat tcctggttgc gcaacaaagc cgatattccg
    27421 ctgactttct gggattatca gcatcacgcg ctggtagcga ccattcgcac ggaagaaccg
    27481 catgatgcgg tggcgcggca ggtttttcat ggcgaaggca ttctggcctt tttgccgctt
    27541 agcgatccgc atctttgctc gattgtctgg tcactgtcgc cagaggaagc gcagcggatg
    27601 cagcaggcag gcgaagacga atttaatcgc gcgttaaata tcgcttttga taatcgcctg
    27661 ggcttatgca aggttgaaag tgagcgcctg gtgttcccgc taacggggcg ttatgcgcgc
    27721 cagtttgccg cacaccgtct ggcgctggtg ggcgacgccg cgcatactat tcacccgctg
    27781 gcggggcagg gggtaaatct cggctttatg gatgctgcag agctgattgc tgaactgaaa
    27841 cggttgcatc gtcagggtaa agacatcggg cagtacattt acctgcgtcg ctatgagcgt
    27901 agccgtaagc acagtgcggc gctgatgctg gctggtatgc agggattccg cgatctgttt
    27961 tccggtacca atccggcgaa aaaattgctg cgtgatattg gtttaaaact ggccgacacg
    28021 cttcctggcg ttaaaccaca acttatccgc caggcaatgg gattaaacga tttgcctgaa
    28081 tggctgcgtt aaaaatttct cctctgttgt ttatttgata cccatcacac tttcatctcc
    28141 cggttttttc gccgggagat tttcctcatt tgaaataaac taatttcacc tccgttttcg
    28201 cattatattt tctaatgtca tgattttttg atttagtgtt ttttgacatt tttttagttc
    28261 ttaatattgc attaatcaaa ttaactttct catctgttta tctttatata gagatttgtt
    28321 cattttttgg ttttttattc tgttgcgatt tttgcatttt ttaaccataa gctaatgtga
    28381 tgatcaattt taccttatgg ttaacagtct gtttcggtgg taagttcagg caaaagagaa
    28441 cgattgcgtt ggggaccggg agtggctccg atgctgggtt tcgtggtgat aatttcgcca
    28501 tggaaaagtt gtcagccccg cttattcaat gaggacaaga tggcacaaca gactcctttg
    28561 tacgaacaac acacgctttg cggcgctcgc atggtggatt tccacggctg gatgatgccg
    28621 ctgcattacg gttcgcaaat cgacgaacat catgcggtac gtaccgatgc cggaatgttt
    28681 gatgtgtcac atatgaccat cgtcgatctt cgcggcagcc gcactcggga gtttctgcgt
    28741 tatctgctgg cgaacgatgt ggcgaaactc accaaaagcg gcaaagccct ttactcgggg
    28801 atgttgaatg cctctggcgg tgtgatagat gacctcatcg tctactactt tactgaagat
    28861 ttcttccgcc tcgttgttaa ctccgccacc cgcgaaaaag acctctcctg gattacccaa
    28921 cacgctgaac ctttcggcat cgaaattacc gttcgtgatg acctttccat gatcgccgta
    28981 caagggccga atgcgcaggc aaaagctgcc acactgttta atgacgccca gcgtcaggcg
    29041 gtggaaggga tgaaaccgtt ctttggcgtg caggcgggcg atctgtttat tgccaccact
    29101 ggttataccg gtgaagcggg ctatgaaatt gcgctgccca atgaaaaagc ggcggatttc
    29161 tggcgcgcgc tggtggaagc gggcgttaag ccatgcggcc tgggcgcgcg tgacacgctg
    29221 cgtctggaag cgggtatgaa tctttatagt caggagatgg acgaaaccat ctctccttta
    29281 gccgccaata tgggctggac tatcgcctgg gaaccggcag atcgtgactt catcggtcgt
    29341 gaagccctgg aagtgcagcg tgagcatggc acagaaaaac tggttggtct ggtgatgacc
    29401 gaaaaaggcg tgctgcgtaa tgaactgccg gtacgcttta ccgatgcgca gggcaaccag
    29461 catgaaggca ttatcaccag cggtactttc tccccgacgc tgggttacag cattgcgctg
    29521 gcgcgcgtgc cggaaggtat tggtgaaaca gcgattgtac aaattcgcaa ccgtgaaatg
    29581 ccggttaaag tgacgaaacc tgtttttgtg cgtaacggta aagccgtcgc gtgatttact
    29641 tttttggaga ttgattgatg agcaacgtac cagcagaact gaaatacagc aaagaacacg
    29701 aatggctgcg taaagaagcc gacggcactt acaccgttgg tatcaccgaa catgcccagg
    29761 agctgttagg cgatatggtg tttgttgacc tgccggaagt gggcgcaacg gttagcgcgg
    29821 gcgatgactg cgcggttgcc gaatcagtaa aagcggcgtc agacatttat gcgccagtaa
    29881 gcggtgaaat cgtggcggta aacgacgcac tgagcgattc cccggaactg gtgaacagcg
    29941 aaccgtatgc aggcggctgg atctttaaaa tcaaagccag cgatgaaagc gaactggaat
    30001 cactgctgga tgcgaccgca tacgaagcat tgttagaaga cgagtaacgg ctttattcct
    30061 cttctgcggg agaggatcag ggtgaggaac ttttacgcct caccctcact ctcttcgcaa
    30121 ggagagaggt tcacaattca ctgcacgttt caggaaccat cgctcatgac acagacgtta
    30181 agccagcttg aaaacagcgg cgcttttatt gaacgccata tcggaccgga cgccgcgcaa
    30241 cagcaagaaa tgctgaatgc cgttggcgca caatcgttaa acgcgctgac cggccagatt
    30301 gtgccgaaag atattcagct tgcgaccccg ccgcaggttg gcgcaccggc gaccgaatac
    30361 gccgcgctgg cggaactcaa ggctattgcc agtcgcaata aacgcttcac gtcttacatc
    30421 ggcatgggtt acaccgccgt gcagctaccg ccggttattc tgcgtaacat gctggaaaat
    30481 ccgggctggt ataccgcgta tactccgtat caacctgaag tctcccaggg ccgccttgaa
    30541 gcactgctca acttccagca ggtaacgctg gatttgaccg ggctggatat ggcctctgcc
    30601 tctcttctgg acgaggccac tgctgccgcc gaagcaatgg cgatggcgaa acgcgtcagc
    30661 aaactgaaaa atgccaaccg cttcttcgtt gcttctgacg ttcatccgca aacgctcgat
    30721 gtggtccgta ctcgtgctga aacctttggt tttgaagtga ttgtcgatga cgcgcaaaaa
    30781 gtgctcgacc atcaggacgt cttcggcgtg ctgttacagc aggttggcac aaccggtgaa
    30841 atccacgact acactgcgct catcagcgaa ctgaaatcac gcaaaattgt ggtcagtgtc
    30901 gccgccgata ttatggcgct ggtgctgtta actgcgccgg gtaaacaggg cgcggatatt
    30961 gtctttggtt cggcgcaacg cttcggtgtg ccgatgggct acggtggccc acacgcagca
    31021 ttctttgcgg cgaaagatga atacaaacgc tcaatgccgg gccgtattat cggtgtatcg
    31081 aaagatgcag ctggcaatac cgctctgcgc atggcgatgc agactcgcga gcaacatatt
    31141 cgccgtgaga aagcgaactc caacatttgt acttcccagg tactgctggc aaacatcgcc
    31201 agcctgtatg ccgtttatca cggcccggtt ggcctgaaac gtatcgctaa ccgcatccac
    31261 cgtctgaccg atatcctggc ggcgggcctg caacaaaaag gtctgaaact gcgtcatgcg
    31321 cactatttcg acactttgtg tgtggaagtg gcagacaagg cgggcgtact ggcgcgtgcc
    31381 gaagcggctg aaatcaacct gcgtagcgat atcctgaacg cggttgggat cacccttgat
    31441 gaaaccacca cgcgtgaaaa cgtgatgcag cttttcagcg tactgctggg ggataaccac
    31501 ggcctggaca tcgacacgct ggacaaagac gtggctcacg acagccgctc tattcagcct
    31561 gcgatgctgc gcgacgacga aatcctcacc catccggtgt ttaatcgcta ccacagcgaa
    31621 accgaaatga tgcgctatat gcactcgctg gagcgtaaag atctggcgct gaatcaggcg
    31681 atgatcccgc tgggttcctg caccatgaaa ctgaacgccg ccgccgagat gctcccaatc
    31741 acctggccgg aatttgccga actgcacccg ttctgcccgc cggagcaggc cgaaggttat
    31801 cagcagatga ttgcgcagct ggctgactgg ctggtgaaac tgaccggtta cgacgccgtt
    31861 tgtatgcagc cgaactctgg cgcacagggc gaatacgcgg gcctgctggc gattcgtcat
    31921 tatcatgaaa gccgcaacga agggcatcgt gatatctgcc tgatcccggc ttctgcgcac
    31981 ggaactaacc ccgcttctgc acatatggca ggaatgcagg tggtggttgt ggcgtgtgat
    32041 aaaaacggca acatcgatct gactgatctg cgcgcgaaag cggaacaggc gggcgataac
    32101 ctctcctgta tcatggtgac ctatccttcc acccacggcg tgtatgaaga aacgatccgt
    32161 gaagtgtgtg aagtcgtgca tcagttcggc ggtcaggttt accttgatgg cgcgaacatg
    32221 aacgcccagg tcggcatcac ctcaccgggc tttattggcg cggacgtctc gcaccttaac
    32281 ctgcataaaa ctttctgcat tccgcacggt ggcggcggcc ctggtatggg accgatcggc
    32341 gtgaaagcgc atctggcacc gtttgtgcca ggtcatagcg tggtacaaat tgaaggcatg
    32401 ttaacccgtc agggcgcggt ttctgcggca ccgttcggta gcgcatccat tctgccaatc
    32461 agctggatgt acatccgcat gatgggcgca gaagggctga aaaaagcaag ccaggtggca
    32521 attcttaacg ccaactatat tgccagccgc ctgcaggatg ccttcccggt gctgtatacc
    32581 ggtcgcgacg gtcgcgtggc gcacgaatgt attctcgata ttcgcccgct gaaagaagaa
    32641 accggcatca gcgaactgga tattgccaag cgtctgatcg actacggttt ccacgcgccg
    32701 accatgtcgt tcccggtggc gggtacgctg atggttgaac cgactgaatc tgaaagcaaa
    32761 gtggaactgg atcgctttat cgacgcgatg ctggctatcc gcgcagaaat tgaccaggtg
    32821 aaagccggtg tctggccgct ggaagataac ccgctggtga acgcgccgca cattcagagc
    32881 gaactggtcg ccgagtgggc gcatccgtac agccgtgaag ttgcggtatt cccggcaggc
    32941 gtggcagaca aatactggcc gacagtgaaa cgtctggatg atgtttacgg cgaccgtaac
    33001 ctgttctgct cctgcgtacc gattagcgaa taccagtaat tcactgattc gactatcttc
    33061 taaaggcgct tcggcgcctt tttagcttgt taataaagtg cgctttgtgt gtcggatgaa
    33121 gcgtaaatat aaactcctgg agttcaatat atgacaggag cgccaggcct gatgagcggg
    33181 gcgcatcagg cacatcgggt aattaaagat tctcgccgtt gctggcaatc acctctttgt
    33241 accagtcaaa gctcttctta cgtgaacgcg acatatcgcc agtaccgtcg tcatgtttat
    33301 tcacatagat aaagccgtag cgtttgctgt actgcccggt ggtaaacgac acgcagtcga
    33361 tgcagcccca cggtgtgtag cccatcagat ccacgccatc gtaagtcacc gcttttttca
    33421 tctcttcaat atgggcgcgc aggtagtcaa tgcggtagtc gtcgttgatg ctgccatctt
    33481 cttccacttt gtcataagcg ccaaaaccgt tttcgacaat aaacagcggc ttctgataac
    33541 gctcatacag ttcgcaaagt gcgtaacgca gacccacagg atcaatctgc cagccccagt
    33601 cagacgcttt aacatacggg tttggtacgc tgccttcaaa accagagatc gcatcgccgg
    33661 tgccgccttc ggccttcacc gcgttggtca tgtaatagct gaaaccgaga taatcgcagg
    33721 tgccttcgcg cagcacatcg agatcgccgt cttccatttt gatgttaaat ccgcgacgct
    33781 cccactcgtt caacacatag gacgggtaat agccacgcag ttgcacatcg gtaaagacgt
    33841 agcgttcgcg catcgactcc tgggcgaaca tcacatcgtc cggattacag gagtaagggt
    33901 agagcggcac catcgccagc atacagccga ctttcatttc cgggttaatg cgacgcgcgg
    33961 ctttcaccgc cagggcgctg gcgacaaact ggtgatgcag cacctggtac atcgtctctt
    34021 ccgggttttc atgctcggta tacaccacgc cggagcagca gtaaccgaac agcggtgcac
    34081 gccagttacg ctggttgtta atttcgttga aggtcatcca gtatttgact ttatgcttat
    34141 agcgttcaaa taccacttct gcgaaacgga caaagaaatc aaccacttta cggttggtcc
    34201 agctaccgta ttgctgcacc agatgcagcg gcatttcaaa gtgggagagg gtgatcaccg
    34261 gttcgatgtt gtatttcagc agttcatcga acatatcatc gtagaacttc agcccttctt
    34321 cgtttggctg agcttcatcg ccttttggaa aaatgcgcgt ccaggcgatg gatgtacgaa
    34381 aacatttgaa gcccatttcg gcaaatagct tgatgtcttc cttgtaatga ccataaaaat
    34441 caacggcttc atggttcgga tagtattttc ctggcaacac ttctttggtg atttcgcgcg
    34501 gcacgccgtg tgcgccaccg gtcagaacgt cacaaatgct cggccctttg ccgcctttgt
    34561 tccagccgcc ttcgacctga tgagcggcaa ctgcgccgcc ccataagaaa tctttcggta
    34621 aggtgagttt tttcactatc ataggctcca gatgagtcaa ttcttacagg aaatagtacc
    34681 atgcagccaa acgacatcac tttttttcaa cgtttccagg atgacattct ggctgggcgt
    34741 aaaaccatca ccatccgcga cgagtctgaa tcgcacttca aaacgggtga tgtgcttcgt
    34801 gtcgggcgtt ttgaagatga cggttatttt tgcacgattg aagtcaccgc aacctcaacc
    34861 gtaacgctgg atacactgac ggaaaaacat gcagagcagg aaaatatgac cctgactgat
    34921 ctgaaaaagg tcattaccga tatctatccc ggtcagacac agttttatgt gattgaattt
    34981 aaatgccttt aaattgaact cacgcctgtt agttgaaatt tcgcatttaa tttcatgatt
    35041 tttcggagat tagtctcaaa tcagaattta ttttagctaa caggtgttca ctggaactat
    35101 tctcagttac gctggagagg tatacatgtg cgtgtacgtt tctccggagt aagttatggt
    35161 tcagaagccc ctcattaagc agggatattc gctggcagag gaaattgcca acagcgtcag
    35221 tcacggcatt gggttggtgt ttggtatcgt tgggctggtg ttgctactgg ttcaagcggt
    35281 ggatctcaat gccagcgcca cagcgataac cagttacagc ctctatggcg gcagtatgat
    35341 cctgctgttc ctcgcttcga cgctctatca cgccattcct catcaacggg caaaaatgtg
    35401 gctgaagaaa tttgaccatt gcgctattta cctattgatt gccggaacct acacgccgtt
    35461 tttgctggtg gggctggatt ctccgttagc gcgcgggttg atgattgtta tctggagcct
    35521 ggcattgctg gggattctgt tcaaactgac catcgcgcac cgattcaaaa ttttatctct
    35581 ggtgacctat ctggcgatgg gctggctgtc gctggtggtg atttatgaaa tggcaattaa
    35641 gcttgcggcg ggcagcgtta ccttactggc tgtaggcggc gtggtttact cgctcggggt
    35701 gattttctac gtctgcaaac gcattcccta caaccatgcc atctggcacg gctttgtgct
    35761 cggcggcagc gtgtgccact ttctggcgat ctatttatat attgggcagg cgtaatgggt
    35821 gtgcctttgt aggcctgaaa agacacaccc ttgtcgcatc aggcgataaa tgcatgatgc
    35881 gacacagaga tttactcttc gagtgaatac ggcagcggct cgatatgcaa tgtattcgca
    35941 tcgtcacgca cgcggaagat gctgtccggt tccatatcgt tattcatgac gacctgtacc
    36001 acgacctgac catcttccag ttttaccgca gccagcacgg ttccggtacg acgccagttc
    36061 tcgcccattt tcagctctaa gtcttcaccg gcttccggca gtcggctggc gctacccgtc
    36121 agcaaccaga gcgcgcgttt gttggcacca cggaatttag ctcgcgccac catctcttgt
    36181 ccggtatagc agcctttttt aaagctaatg ccgcccagcg cctggaggtt ggtcgcctgt
    36241 gggataaact gcccgctgtt ggcggcatca atcaccggga aacccgcttc aatgtttaat
    36301 gccagccact gttggctatt gttcagttcc gcttcaccgc gcagtttatc ggtcagcata
    36361 ttggcagtgg cttcatcggt tacgatcagg aaacgttctg cagggtgttc aaaccatagc
    36421 aaagtggtcg cgccttcttt aactacctgt ttttctttcg aaggcagttc actaaagata
    36481 tttgccagcg cggcgcgcgc ctgaaaaccg gcaacaccaa gcagcacacg ctcatcgtcc
    36541 ggcgcgatgg tcactttaga gaacaccgca tattttttca gttcagtcag ctgcggttca
    36601 cgcacactgc gccgttcaat ccatgcaaag ccatcgccgt cgcggaacag acgtaaattg
    36661 ctccacattt tacctttggc gtcgcaatgg gcggcgagca gatgctgatt ttctgtcatc
    36721 tggctgacat ctgctgtcac ctgaccctgc atatattttt cgctgtccgc gccagtaatg
    36781 gtggcaagcg cccagtcatc aagcgttatc agcgtcagcg gtaaacgggc agacgccgta
    36841 ggctgacggg gaggaaaagg tgtaaaagcc ataatagagt cctgagttgc ttaacggcgt
    36901 attaatgact aatggtaaaa gagctatgag gcaatgcaag tgctttcact acgcgatctg
    36961 tgccttctga tatgctttgc gaggcgactt ccaaaaaaat gaagcccttg aattgttgtg
    37021 aattaatttc gcgatcgcga ttgtgtttac tgatattccc gtaaaagaag tcgaaaaaca
    37081 cgttacaata gccgggtact ggattttcgt gagacaggaa gaagatggac attaacaaca
    37141 aagcccgcat tcattgggca tgccgccgtg gtatgcgcga actcgatatt tcaatcatgc
    37201 cgtttttcga acatgagtac gacagcttaa gcgatgacga aaaacgcatc tttattcgtc
    37261 tgctggaatg tgacgatccg gacctgttta actggctgat gaatcacggt aaaccagccg
    37321 atgcagaact ggaaatgatg gtccgactca tccagacacg gaaccgggaa cgtggtcctg
    37381 tggcaatctg atttgcgcgt ctcctggcgc gcacagtggc tttccttgct gattcatggg
    37441 ctggttgccg ctgttatttt actcatgccc tggccgctca gttacacccc gttatggatg
    37501 gtgttacttt cgctggtggt gtttgattgc gttcgcagcc agcggcgcat taatgctcgc
    37561 cagggggaaa ttcgcttgtt gatggacggg cgtttgcgtt ggcaagggca ggagtggagc
    37621 atcgtcaaag cgccgtggat gattaagagc ggcatgatgc tgcgtttacg ttctgacagc
    37681 ggaaaacggc aacatttatg gctggcagct gacagcatgg acgaagccga atggcgggat
    37741 ttacggcgga tattgttaca acaagagacg caaagataaa gatgacggca aagcatatgt
    37801 gctaagccgt cagtgacatc aggcgtaatg ctctgccatt tcgttgagga tttgctcgca
    37861 ccagctctga atacgctcgt cgctaaggtc atactggtta gtttcatcca gcgccagacc
    37921 cacgaacagt tgcccgtcag caatcaccgg tttcgggctg gtaaattcat atccttccgt
    37981 tggccagtag ccgacgaact tcacgccttt ggtcgagagt ttgtcatgca gcataccgag
    38041 cgcatcgagg aaccactcgc cgtatcccag ttgatcgcca agcccataca gcgcaacaat
    38101 tttaccttcg aggttcaggt cgtcgagctg atcccagacg gcttcccagt cttcctggat
    38161 ttcgccaaaa tcccaggtcg ggatacccag aatgagcaca tcgtactgct ccattaattt
    38221 cggggagtcg tccttgaggt tatgtaaggt caccagttct gggccgataa tatcgcggat
    38281 tttttctgcc gccatttcgg tgtaacaggt gctggaaccg taaaaaagac ccatattcat
    38341 aacgtaagct tctcgattct gctgtggctt ttgcggctag tgtatcagaa tcgctttagt
    38401 cacggcacaa taaggcataa tggatcaaat gagtgaccat aaggggcgca agtgaaacag
    38461 gatctggcac gcatcgagca gtttcttgat gctctgtggc tggaaaaaaa tctggctgaa
    38521 aatacgttga acgcttaccg tcgcgatctg tcaatgatgg tggagtggtt gcatcaccgc
    38581 gggttgacgc tggcgacggc gcaaagtgac gatttgcagg cattactggc agaacggctg
    38641 gagggcgggt ataaagccac cagctcagcg cgtttgctga gtgcagtgcg ccgattgttc
    38701 cagtatcttt atcgcgaaaa gtttcgtgaa gatgatccca gtgcgcatct cgcttcaccg
    38761 aaattgcccc agcgtttgcc aaaagattta agcgaagcgc aggtcgaacg tttattacag
    38821 gcaccattaa ttgatcagcc actggagcta cgcgataaag ccatgcttga agtgttgtat
    38881 gctaccggac tgcgtgtctc tgaactggtc gggctgacaa tgagtgatat cagcctgcgt
    38941 cagggcgtgg tgcgggtcat tggtaaaggc aacaaagagc gcctggtgcc gttaggtgaa
    39001 gaggcggttt actggctgga aacctatctg gaacatgggc gtccgtggct gttgaatggt
    39061 gtgtcaattg acgtgttgtt tcccagccag cgtgcgcagc agatgacgcg acagaccttc
    39121 tggcaccgca ttaaacatta tgccgtgctg gcgggtatcg acagcgaaaa gctgtcaccg
    39181 catgtgttgc gtcacgcttt tgccactcac ttattaaatc atggtgcgga tttgcgcgtg
    39241 gtgcagatgc tactgggcca cagcgatctc tccaccacgc aaatttatac gcatgtcgct
    39301 accgagcgtc tgcggcaact tcatcaacag catcacccgc gggcgtgatg tctgaaaaga
    39361 acaggaaaat ttatgaagaa aggttttatg ttatttacct tgttggcagc gttttcaggc
    39421 tttgttcagg ctgatgacgc ggcaattcaa caaacgttag ccaaaatggg catcaaaagc
    39481 agcgatattc agcccgcgcc cgtagctggc atgaagacag ttctgactaa cagcggtgtg
    39541 ttgtacatca ccgatgatgg taaacatatc attcaggggc caatgtatga cgttagtggc
    39601 acggctccgg tcaatgtcac caataagatg ctgttaaagc agttgaatgc gcttgaaaaa
    39661 gagatgatcg tttataaagc gccgcaggaa aaacacgtca tcaccgtgtt tactgatatt
    39721 acctgtggtt actgccacaa actgcatgag caaatggcag actataacgc gctggggatc
    39781 accgtgcgtt atcttgcttt cccgcgccag gggctggaca gcgatgcaga gaaagaaatg
    39841 aaagctatct ggtgtgcgaa agataaaaac aaagcgtttg atgatgtgat ggcaggtaaa
    39901 agcgtcgcac cagccagctg cgacgtggat attgccgacc attacgcact tggcgtccag
    39961 cttggcgtta gcggtactcc ggcagttgtg ctgagcaatg gcacacttgt tccgggttac
    40021 cagccgccga aagacatgaa agaattcctc gacgaacacc aaaaaatgac cagcggtaaa
    40081 taattcgcgt gaaacaacag atacaacttc gtcgccgaga agtcgatgaa acggccgact
    40141 tgcccgctga attgcctccc ttgctgcgcc gtttatatgc cagccggggt gtgcgtagcg
    40201 cgcaggaact ggaacgcagt gttaaaggca tgctgccctg gcagcaactg agcggcgtcg
    40261 aaaaggccgt tgagatcctt tacaacgctt ttcgcgaagg aacgcggatt attgtggtcg
    40321 gtgatttcga cgccgacggt gcgaccagca cggctctaag cgtgctggcg atgcgctcgc
    40381 ttggttgcag caatatcgac tacctggttc caaaccgttt cgaagacggt tacggcttaa
    40441 gtccggaagt agtcgatcag gctcatgccc gtggcgcgca gttaattgtc acggtggata
    40501 acggtatttc ctcccatgcg ggggttgaac acgctcgttc gttgggcatt ccggttattg
    40561 ttaccgatca ccatttgcca ggcgacacat tacccgcagc ggaagcgatc attaacccta
    40621 acttgcgcga ctgtaatttc ccgtcgaaat cactggcagg cgtgggtgtg gcgttttatc
    40681 tgatgctggc gctgcgcacc tttttgcgcg atcagggctg gtttgatgag cgcggtattg
    40741 caatccctaa tctggcggag ctgctggatc tggtggcgct ggggacggtg gcggatgtag
    40801 tgccactgga cgccaataac cgcattctga cctggcaggg gatgagtcgc atccgtgccg
    40861 gaaagtgccg tccggggatt aaagcgctgc tggaagtggc caatcgcgat ccacaaaaac
    40921 tcgccgccag cgatttaggt tttgcgctgg ggccacgtct caatgctgcc gggcgactgg
    40981 acgatatgtc cgttggcgtg gcgctgttgc tgtgcgacaa catcggcgaa gcgcgcgtgc
    41041 tggcaaatga actcgatgcg ctaaaccaga cgcgaaaaga gatcgaacag ggaatgcaag
    41101 ttgaagccct gaccttgtgc gagaaactgg agcgcagccg cgacacacta cccggcgggc
    41161 tggcaatgta tcaccccgaa tggcatcagg gcgttgtcgg tattctggct tcgcgcatca
    41221 aagagcgttt tcatcgtccg gttatcgcct ttgcgccagc tggtgatggt acgctgaaag
    41281 gttccggtcg ctccattcag gggctgcata tgcgtgatgc gctggagcga ttagacacac
    41341 tctaccccgg catgatgctc aagtttggcg gtcatgcgat ggcggcgggt ttgtcgctgg
    41401 aagaggataa attcgaactc tttcaacaac ggtttggcga actggttacg gagtggttgg
    41461 acccttcgct attgcaaggc gaagtggtgt cggacggtcc gttaagtccg gccgaaatga
    41521 ccatggaagt ggcgcagctg ttgcgcgatg ctggcccgtg ggggcagatg ttcccggagc
    41581 cgctgtttga tggtcatttt cgtctgctgc aacagcggct ggtgggcgaa cgtcatttga
    41641 aagtcatggt cgaaccggtc ggcggcggtc cactgctgga cgggattgct tttaatgtcg
    41701 ataccgccct ctggccggat aacggcgtgc gcgaagtgca actggcttac aggctcgata
    41761 tcaacgagtt tcgcggcaac cgcagcctgc aaattatcat cgacaatatc tggccaattt
    41821 agcgtcatct tctctataaa aaagagcgtg gattgggtac aatcccgctc ttatcaccgc
    41881 attttgacta gctcaataaa agaaatcaga ccatgtttga aattaatccg gtaaataatc
    41941 gcattcagga cctcacggaa cgctccgacg ttcttagggg gtatctttga ctacgacgcc
    42001 aagaaagagc gtctggaaga agtaaacgcc gagctggaac agccggatgt ctggaacgaa
    42061 cccgaacgcg cacaggcgct gggtaaagag cgttcctccc tcgaagccgt tgtcgacacc
    42121 ctcgaccaaa tgaaacaggg gctggaagat gtttctggtc tgctggaact ggctgtagaa
    42181 gctgacgacg aagaaacctt taacgaagcc gttgctgaac tcgacgctct ggaagaaaaa
    42241 ctggcgcagc ttgagttccg ccgtatgttc tctggcgaat atgacagcgc cgactgctac
    42301 ctcgatattc aggcggggtc tggcggtacg gaagcacagg actgggcgag catgcttgag
    42361 cgtatgtatc tgcgctgggc agaatcgcgt ggtttcaaaa ctgaaatcat cgaagagtcg
    42421 gaaggtgaag tggcgggtat taaatccgta acgatcaaaa tctccggcga ttacgcttat
    42481 ggctggctgc gtacagaaac cggcgttcac cgcctggtgc gtaagagccc gtttgactcc
    42541 ggcggtcgtc gccacacgtc gttcagctcc gcgtttgttt acccggaagt tgatgatgat
    42601 atcgatatcg aaatcaatcc ggcggatctg cgcatcgacg tttatcgcgc atctggcgcg
    42661 ggcggtcagc acgttaaccg taccgaatct gcggtgcgta ttactcacat cccaaccggg
    42721 atcgtgaccc aatgccagaa cgaccgttcc cagcacaaga acaaagacca ggccatgaag
    42781 cagatgaaag cgaagcttta tgaactggag atgcagaaga aaaatgccga gaaacaggcg
    42841 atggaagata ataaatccga catcggctgg ggcagccaga ttcgttctta tgtccttgat
    42901 gactcccgca ttaaagatct gcgtaccggg gtagaaaccc gcaacacgca ggccgtgctg
    42961 gacggcagcc tggatcaatt tatcgaagca agtttgaaag cagggttatg aggaaccaac
    43021 atgtctgaac aacacgcaca gggcgctgac gcggtagtcg atcttaacaa tgaactgaaa
    43081 acgcgtcgtg agaagctggc gaacctgcgc gagcagggga ttgccttccc gaacgatttc
    43141 cgtcgcgatc atacctctga ccaattgcac gcagaattcg acggtaaaga gaacgaagaa
    43201 ctggaagcgc tgaacatcga agtcgccgtt gctggccgca tgatgacccg ccgtattatg
    43261 ggtaaagcgt ctttcgttac cctgcaggat gtgggtggcc gcattcagct gtacgttgcc
    43321 cgtgacgatc tgccggaagg cgtttacaac gagcagttca aaaaatggga cctcggcgat
    43381 atcctcggcg cgaaaggtaa actgttcaaa accaaaactg gcgaactgtc tatccactgt
    43441 accgagctgc gtctgctgac caaagcactg cgtccgctgc cggataaatt ccacggcttg
    43501 caggaccagg aagcgcgcta tcgtcagcgt tatctggatc tcatctctaa cgatgaatcc
    43561 cgcaacacct ttaaagtgcg ctcgcagatc ctctctggta ttcgccagtt catggtgaac
    43621 cgcggcttta tggaagttga aacgccgatg atgcaggtga tccctggcgg tgccgctgcg
    43681 cgtccgttta tcactcacca taacgcgctg gatctcgaca tgtacctgcg tatcgcgccg
    43741 gaactgtacc tcaagcgtct ggtggtcggt ggcttcgagc gtgtattcga aatcaaccgt
    43801 aacttccgta acgaaggtat ttccgtacgt cataacccag agttcaccat gatggaactc
    43861 tatatggctt acgcagatta caaagatctg atcgagctga ccgaatcgct gttccgtact
    43921 ctggcacagg atattctcgg taagacggaa gtgacctacg gcgacgtgac gctggacttc
    43981 ggtaaaccgt tcgaaaaact gaccatgcgt gaagcgatca agaaatatcg cccggaaacc
    44041 gacatggcgg atctggacaa cttcgactct gcgaaagcga ttgctgaatc tatcggcatc
    44101 cacgttgaga agagctgggg tctgggccgt atcgttaccg agatcttcga agaagtggca
    44161 gaagctcatc tgatccagcc gaccttcatt actgaatatc cggcagaagt ttctccgctg
    44221 gcgcgtcgta acgacgttaa cccggaaatc acagaccgct ttgagttctt cattggtggt
    44281 cgtgaaatcg gtaacggctt tagcgagctg aatgacgcgg aagatcaggc gcagcgcttc
    44341 ctggatcagg ttgccgcgaa agacgcaggc gacgacgaag cgatgttcta cgacgaagat
    44401 tatgtcaccg cactggaaca cggcttaccg ccgacagcag gtctgggaat tggtatcgac
    44461 cgtatggtaa tgctgttcac caacagccat accatccgcg acgttattct gttcccggcg
    44521 atgcgtccgg taaaataagc attacgttat gctcacaacc ccggcaaatg tcggggtttt
    44581 ttatttaaga tgggtaaatg cagataatcg ttttctggct tcgcgatttg tcgcctgcat
    44641 caccatccac ggactgaacg cccacggtgt ggcatcaata ccgtgtaata catctgctaa
    44701 atcacaccat tgataatcca tcacttcatc atcgttgatc tgtaacgcac tggtcgtgcg
    44761 tgcggcaaat accggacaca cttcattttc cacaatgcca ttcggatcgg tggcgcggta
    44821 gcgaaagtca ggatagatag attcaggagg cgtaatttcc acgccaagct cataacggca
    44881 acggcggatc actgcgtctt cgttgctttc tccctgttgt gggtgcccac aaaccgagtt
    44941 agtccacacg ccaggccatg cttttttgct cagtgcgcgg cgggtaacta ataattgccc
    45001 cttggcatta aacagccaac tggagaacgc gagatgtaag agggtgtctg ccgtgtgtgc
    45061 ggcatacttt tccagcgtac ccgtgggaac tccctgtgca ttcaataaaa tgacgtgttc
    45121 cgtttgcata atttctcaca tgtaattcta atcactcatc attatagata attgaagagt
    45181 gaaatgatta accaagtgag cgtttatcga caaccgcccg ttttgagcgg atgtcgacag
    45241 gtaaaaccat ttaaaaaagc agcatcatat atttgaagcg actttcaatg gccattgcaa
    45301 cacagtcaga aatgacagga atagagagag taaaaagcaa caacagactg aatatagcgc
    45361 gcattaaaag attcatatta ttctccatgc tcatttttct tcagcttgcg catcagaagc
    45421 cacatacgta ctgtcctgac ttttaaatct ttatcagaga cggattcctt caccagatca
    45481 gcatgatgct gatagccatt aaagaagacg ttcagcacaa cggcgctaag ggttgcgagc
    45541 ataatgccgc tatgcagcaa cggttgcagt acggccggta acttagaaaa gaaatcgtga
    45601 gagagcgtcg gagtcatgcc aacgccgaga ctgatcgcca caatatagag gttgtaacgg
    45661 ttagtggtgt agttacagcg cgacagaatt cgaatccctg tcgccagtac catgccgaac
    45721 atcaccagcc cagcaccgcc cagcacaaat tgcggaatgg aggcgaccag caccgccatt
    45781 tttggcacca tgccaaacat gattaatata attcccgaag aaatacacac ccagcggcta
    45841 tgaacgcgcg tcacgctaac caggccaacg ttttgagaaa atgacgtgtg ggggaagctg
    45901 ttaaacgttc cgcctatcat tgtccctaca ccatcgacac gcagcccgcg aataatatcg
    45961 tgtgaagaga gtttacgacc gactatttca cccagtgcca ggaacatccc catcgactcg
    46021 ataaacacga tgattaacac ggcagtcatg gtcagaatgg aaacgggatc gaaaatcggc
    46081 ataccaaatg acatcggcgt gacaatcgca aaccatgaag catcatgtag cccggataaa
    46141 ttgacttcat tcatcatcca cgaaagtaaa aagccaaata caatccccag taatacggcg
    46201 acgttggaca taaatccttt cgcatagcga gtaatgagca agataaaaat taagacggca
    46261 aaggagatcc ccaaataaac gggattacca tattgcggat tccctttacc tccggcggcc
    46321 cagtcaatac ccacctgaat gatgctaagt ccgatagaag taataaccac accggtaacc
    46381 agtggcggga ataaaggcat caagcgaccg ataagtggcg ctaataatgt ggtgataaaa
    46441 cccgcggcga tagtggcacc gaatatcccc agcaggccga tatccgggtt cataccaatg
    46501 gctatcattg gtgttacagc ggcgaaggtc accgacataa tcaccggcag gcggatcccc
    46561 ataaagcggc cgataccgat acattgcaat aatgtgacga tcccgcagca aaagagatcc
    46621 gagctaatga gcatcgcaat agcttctttt gataggccga gtcggtcacc aatcataaga
    46681 ggaacagcga ctgcacctgc gtacatgaca aggacgtgtt gcagaccgag tatgatcagc
    46741 tttcctggtg ataatatgcg gtcaacctca tcagttgggc ggtcttgccc agaagatgag
    46801 ggaagttggg aatctatggc gctcatcagt gtttctcctt tcgcccgcca gagtaaaaag
    46861 caaccgttta tgttcatgaa actgtctaaa cacatgctcg tggagtgcca gtttttgcga
    46921 ggaaggggag ttagagacag gaattgcgga ggcaatcaca taatcgtggt taaaccacga
    46981 aaatgagaag agaacctgac aatggaatta tctgtaattt tctcatattg atatttcatt
    47041 aatcattctg gcatgtcgca ctctcgcatt taatcgtttt tatctggata gcattctttt
    47101 gatcggcaat gttgaaataa tgaaagcggc atagattgag gggcaataat ttgaactcag
    47161 ttgcaaaatt ataattaaaa cggtcatgct acctaatata tgcccgttta ttgcatcgcg
    47221 aaatttgaga agacataact cattaaatga aagggatgca aatgaataag tttatcgctg
    47281 ctgaagctgc ggaatgtata ggctgccatg cttgtgaaat tgcctgtgcg gtggcacata
    47341 atcaagaaaa ctggccgctg agtcacagtg actttcgacc gcgtatccac gttgtaggga
    47401 aaggccaggc tgcgaatccg gtggcctgcc atcactgcaa caatgcccct tgcgttacgg
    47461 cttgtccggt taatgctctg actttccagt ccgatagcgt acaactggac gagcaaaaat
    47521 gtattggttg taaaagatgc gcaatcgctt gcccctttgg cgtcgttgag atggtcgata
    47581 cgattgcaca gaaatgcgac ctttgtaacc agcgcagttc cggcacgcaa gcctgtattg
    47641 acgtctgccc aacacaggcg ttacggctga tggacgataa agggttgcag cagataaagg
    47701 tggcccgcca gcgcaaaacg gcagcaggaa aagcgtcatc agacgctcag ccatctcgca
    47761 gtgcagcgtt gctccccgtt aactcgcgta aaggcgcaga taaaatttca gcgagtgaac
    47821 ggaaaaccca ctttggcgaa atctattacg ggctggatcc acaacaagcg acttatgaga
    47881 gtgaccgctg tgtttattgc gccgaaaaag ctaactgcaa ctggcattgc ccgctgcata
    47941 acgctattcc ggattacatc cgtctggtgc aggaaggaaa gattattgaa gcggcagaac
    48001 tttgccacca gaccagttcc ttacccgaaa tctgcggcag ggtatgtcca caggaccgtc
    48061 tttgtgaagg cgcatgtact ttgaaagatc actctggcgc agtctctatc gggaatctgg
    48121 aacgctacat taccgatacc gcgctggcga tgggctggcg tcccgatgtc agcaaagttg
    48181 ttccccgtag cgaaaaagtg gcggtgattg gcgcagggcc tgcagggtta gggtgtgctg
    48241 atattctggc gcgcgcggga gttcaggttg atgtctttga tcgccatcca gaaattggcg
    48301 gtatgctgac ttttggcata cctcctttca aactcgataa aacggtatta agccagcggc
    48361 gagagatatt caccgcaatg ggaatcgact ttcatcttaa ctgtgaaatt ggccgcgata
    48421 ttacctttag cgatttaact tctgaatatg atgcagtttt catcggcgtg gggacttacg
    48481 ggatgatgcg agcagatctg ccgcatgaag atgcgcccgg tgtcattcag gcgttaccgt
    48541 tcctgactgc ccatacccgc cagctcatgg ggttgccgga gtctgaagag tatccgctga
    48601 cggatgtgga aggtaagcga gtcgtggtat tgggcggtgg cgatacgacc atggattgtt
    48661 tgcggacttc catccgcctc aatgccgcca gcgtgacctg cgcgtatcgt cgtgatgaag
    48721 tcagtatgcc gggctcgcgc aaagaggtgg tcaatgcgcg cgaggaaggt gtcgagtttc
    48781 agttcaatgt tcagccgcaa tatatcgctt gtgatgaaga tggacgctta acggcggtgg
    48841 gcctgattcg taccgctatg ggtgagccgg ggccggatgg tcgccgtcgt cctcgtccgg
    48901 ttgcgggttc agagtttgaa ttgcccgccg atgttctcat tatggccttt ggtttccagg
    48961 cacataccat gccgtggttg cagggcagcg gaattaaact cgataaatgg ggcctgattc
    49021 aaaccggtga cgtcgggtat ttacctaccc agacgcatct gaaaaaagtc tttgctggtg
    49081 gtgatgcagt tcatggcgcg gatctggttg tcactgcaat ggccgcagga aggcaggcgg
    49141 cgcgcgatat gttaactctg tttaatacga aggcatcgtg atgaaatcgt taattatcgt
    49201 taatccggct gactgtattg gctgccgtac ttgtgaagta gcctgtgtgg tcgctcaccc
    49261 ttcagaacag gagttgaatg ccgatgtctt tttaccccgg ctgaaggtac agcgactgga
    49321 cagcatcagt gcgccggtga tgtgccatca gtgtgaaaac gccccttgtg ttggcgcttg
    49381 ccccgtgggg gcgctgacga tgggcgagca ggtggtgcag accaattctg cccgttgtat
    49441 tggctgtcag agttgcgtca gtgcgtgccc gtttggaatg atcaccattc agtcgttgcc
    49501 gggggatacc cgacaacaaa ttgtgaaatg cgatctctgt gaacagcggg aagaggggcc
    49561 agcctgtgtt gaatcttgcc cgacacaggc gctgcagttg cttactgaaa gagaactcag
    49621 gcgagtccgc cagcagcgta ttgttgccag cggtgagaat cctctgtgaa agaaatctcc
    49681 gcgctgaaag gcgcggagaa tctattagat tgcccaacca cccgcgtaaa atgcgaccag
    49741 tgcggcagta atgatcaccg taccaatatt caacttttgc cattcgcgag caaagacacg
    49801 gcctacgacc agtgtcacaa agcccagcat aataccggta acgatattac aagtcagaac
    49861 gatgaacacg gcacacacca ggccagccat tgcgtcaata aaatcgttga aatccagctt
    49921 cgagacgtta cttaacatca gcaaacccac gtacatcaga gcgggtgcag tggcgtaacc
    49981 agggatcaaa aatgacagcg gtgataagaa cagaatcagc aggaataacg cccccactac
    50041 ggttgcggtc aaccctgttt tacccccggc ggcggttcct gccgctgatt cgatataaac
    50101 cgctgcgggc gctgcgccca ccagaccgga gaatattgaa cttactgagt cactggtcag
    50161 ggctttgccg ccattgatga tctggttgtc tttatccaac aaattcgcct gaccggcgac
    50221 ggcacggatg gtgccagtgg catcgaacac tgcggtcatc accaatgcca gtacactcgg
    50281 aagtacggtt ggctggagtg cgcccataat atcgaggctg aaaatcagag acttaccatc
    50341 ttcgccagtc agacttggca tcgccaccag accgtggtat ttcaccgctg ggtcaaagat
    50401 taagccgata atcgaaattg cgataatcac caacaagatc ccgccgggta cgcggcactt
    50461 ctccagaccg aagatgaccg ccagccccag caagctcatc atcaccggga aggaggtaaa
    50521 cgcaccgagc gccactggca agccttcaat cgggtttttg ataaccatac ccacaccgtt
    50581 agcagcaatc agcagcagaa acaggccgat accgatacct gtaccgtgag cgatacccat
    50641 cggtaaatta cgtaagatcc aggtacgcac accggttacg gaaatagcgg tgaagatgac
    50701 gcccatcaga aagaccgcgc ccagtgcgac aggaacgcta atttgttgcc cgagaaccag
    50761 actgaatgcg gtaaacgccg tcaaggaaat cgcgcaacca atcgccattg gcaaattggc
    50821 ccataatccc atcagcaacg agccgaagcc cgcgaccaga caggtggcaa caaacacagc
    50881 tgcgggagga aaacctgctt tgcccagcat tcccggaacg acgataacgg aataaaccat
    50941 ggccagaaag gtcgttaagc cagccagtac ttcctgacga acggtactgc cacgagcggt
    51001 aattttaaaa taattatcca gcgcgccctg tggctttggt gcgtccggtg tttgtaggat
    51061 gtctccagac ataatgatgt cctcacgagg atgttaaagt tttattatta gttgcgttcg
    51121 tacaccagac gaccatcaac gtaggtgcgg tagatcgaac ggtcatcgcc caacgtcatc
    51181 atcacgaaca atttgtcgac taaagaaaca gagttgtcat agcgcagctg ctgtagcgga
    51241 gtggcagtgg gttccatcac cacgaaatca gcctctttgc caggtaaaaa gttgccaatc
    51301 aaatcgtcaa ggcccagaga tttcgctccg ccgagcgtgg ccaggtaaaa cgcttcatat
    51361 gccgagaggc gatagccttg taattgcaat accttgtagg cttcgttcag cgtttgcagc
    51421 atgttaaaag ttgttccggc accgatatcc gttcccatgc ccactttgac tttcttctgc
    51481 catgcttttt tcaagttgaa taagccgctg ccgaggtaaa ggttggaggt cggacagaaa
    51541 gcaatgctgg atttggtttc gctgagacga tcccactctt tttcttcgag atggacgcag
    51601 tgagcaaaga cacagttttt accggtcaga ccgtactgat ggtaaacatc cagataacca
    51661 tcatggtcag gataaagcga tttgacccag gcaatttcat ctttgttttc acagagatgg
    51721 gtatgtaccc acgtatccgg atattcttct ttcaggcgtt gcgccatcgc catctgttca
    51781 ggagatgagg taggggcgaa gcgtggcgta atcgcataca acagacgacc atttttgtgc
    51841 cagcgttcaa tcagttcttt gctttggtga tagctgcttt cggcagtgtc gagcagataa
    51901 tccggtgcgt ttcggtccat catcacctta ccggcaatca tacgcatatt gatatgactg
    51961 gcggcttcaa acagcgcatc aacggattgt ggatgaacag tgccaaacac cagcgccgtg
    52021 gtggttccgt tacgtaaaag ctgcttgatg aagaacgccg acatctcgcg ggcgtactct
    52081 aaatcctcat aacgacgttc agtagggaag gtgtgtttat tcaaccactc cagcaattgt
    52141 tcaccatagg cccccaccat ttcactttgc ggataatgga tatgtgtatc gacaaagccc
    52201 ggtactatca gtttgccgcg atagtcgcgc acgcgaatgg tgtcaggaat ttgatgcttt
    52261 ccgtcttccc attcgccaaa ccattccact tttccctgtt taatgagtaa taaaccatcc
    52321 tcaataaacc gcagcgcaga ggcaatttct tccgggttat cgaccgtacg ggtgacatca
    52381 ataaaactgc ctcgtaccgc ttttaacgtg tgttctcctg acatcattga ctcctttact
    52441 gttaatccat ctcttccgct gaggtaatac caggcagaac gttttcctgt cggtagccac
    52501 cagggaggat aatatttaat aaaatcgcag ttaacccgcc agcacaaata gggttttcaa
    52561 ctaatacata aatagaggct ggcaatattt taaaaatttc gggatcgtag gagacgccaa
    52621 gccctaaacc taaagacgtg gcgacaataa gtgtttctcg gcgctttaaa ccgttggtga
    52681 tgatgatgcg aatccctgcg atggcaatca tggaaaacat caacgtcatt gcgcctccca
    52741 gaactgccga gggtatggtt gtgaagaagc cgccaatcat cggaaacaag ccgaggataa
    52801 ccagcattac cgcgatggtt cgcccgacat aacgtgaagc gacgccagtc atctgaataa
    52861 ccccattatt ttgcgcaaac gtggttaatg gtaatgatcc gacagcggag gcgataacag
    52921 aaaccagacc atctgccagc acgccgcctt tcagtcggga ctgatactct tccccctgaa
    52981 tggggcggcg ggaaaccatt gccgtggcgg tgatatcgcc gactgcttcc agcacgctaa
    53041 gcagataaat cgtgccaacc accaggaact gatggaagct aaaactaaag ccgtatttga
    53101 acggatgcgg gatggtgatt aacggcaaat tgcgcatact gctgaaatcc accatgccca
    53161 ggcataacga tgcgatatag ccgacacata gcccaatggc gatccctccc atgcgtagca
    53221 acggactgcg gcagcagtta aagccgatca ccacaatcaa aaccaataaa ccaacgccga
    53281 gatgttcgta attgccgaac gtaccgctgc ttttggctgc aaatcctcca ccaaaatcaa
    53341 taatgccgac tttaatcagg cttaagccga tcatcagcac cacaatgccg ctgacggtag
    53401 gcgtaataac ccgacgtaaa tagggcagga taaaagaaga gccgacaacc agaaatgcgc
    53461 caacgaagga gacgccgagg agcgacgaca tgattaactc ttcgtgaaaa ccgtcgcttt
    53521 tcatgctgct gcccagcgca atcatgaccg taacaaatga aaaattgact gactgaattg
    53581 aaagtaaacc agaaccgacg atgccgtagc ggtttacttg taaccaggta ccaataccag
    53641 aggcgatcat cgccatagaa acaagatagg cagttgtttc agcggaaagc tgtaaggccg
    53701 cacctacgat taacgcgggg gttaccatcg gaacgaaaat tgccaacaga tgggtaatgg
    53761 cacctacgag agcctgatga aagggaggac gatcctccag ttcaaatata aggtcagaac
    53821 ctgcatggtt tatatcagac atcccttcac tccttttaaa gtaaatttga tcatctgaat
    53881 tagtaaaata aattcccacg aatttggggc gcaggacgcc ttgagaacgg cttatctcat
    53941 tttcgcagtc actatttagt catcgttcaa acagagaaac aggccgccga agcggccatt
    54001 tattacttat attttttcca gcgcggtgag tattttctcc ggtgtgaaat gccattcgcg
    54061 taaccagatg ccgcaggcat cgtgaattgc ggtagcaatc gccggagcgg cgccatttac
    54121 accgatttcc gagatcgatt tcgccccgaa tgggccgact ttatcgtcgc tcggcaccag
    54181 cacagcgcgg aaatcacgcg gaatgtcacc aattttcggt gcgccgtaac tgcgtaaatc
    54241 acgcgttaac gggtgacctt cggcgtcata aatgatctct tcgctcatac tgtggccgat
    54301 agcacgcagg gtggcaccgt agatttgtcc cagcgctaac tctggattga ccggtgtacc
    54361 gcagtccagc aaggcgtaga atttatccag gcggatttca cccgtacgcg tgttgacggc
    54421 aacttcagcg aagtttgcgc catacgggaa ggcgaaatca ggcgtgatat aactgccagt
    54481 tcccaccagt gaaccaaagc cggtgccggt ttcgccttta tgggctatct ctccgaaact
    54541 aacttcgcct ttcttgccgc gaacgacgcc cggcgttgct agttgaacat ctgccactgg
    54601 ctcacccaac atttgcgcgc cgtggaacag gattttctcc cgcagatttt ccgctgccaa
    54661 acgcgcagcg ttacccgaga agcaagtacc ggacgaggca tatgcgcctt tatcaaacaa
    54721 cgcatgatcg gtatcaccgg agataacatg cacgtcctgc ggggggcagt gcagcacttc
    54781 tgctgccagt ttcgtcacca cagtatccaa accagtacca atatccgcac cgccagaatg
    54841 aacgataaag gtaccgtctg attccagttt gatcatgcag ttagcctgat cgatatccgg
    54901 gatacccgat ttctgcatga taatggcgac gccacgaccg atgtgccagt caccgttttg
    54961 cggtttcggt gaagaccatt ggatcatctc gcgaccctga cgcaggatct cttccagtgc
    55021 gcagctggcg gcggaaggaa cggatgtcgg cgctttacct tcaccgattg caccgagaat
    55081 tttcagctct tgcccttcgt gtacccggtt acgttcgata atttccagtt ggtcgatctg
    55141 taactgttca gccagttccg ctaatgccat ggtgatagcg aagttacctt tcggcgcgcc
    55201 ataaccctga taagcaccat ttgggcaaat gttgctgtag taggtggtga cctgaaaatc
    55261 gacgttatcg catggatata acggcagcga cagcgccggt ccgttacacg gtacggtgag
    55321 tgagtggtta ccgtaaggac ccgtgttggc gcggaaatcc atcttcactg ccgtcaggcg
    55381 accatctttt ttcgcgccca gtttgacggt gactttcgcg acgtgacgag aggtgttagc
    55441 aataaactct tcttcacggg tgtagcggaa aagtaccgga cgcccggtca cgcaagttgc
    55501 ccaggcgcac acttcttcca gcaggatgtc ctgtttagag ccaaaaccgc cgcctacacg
    55561 ttctttaatg acatgaactt tatgttgttt catgcccacg aggcgcgcga cctggcggcg
    55621 taagtgccat ggtacctggg tggaggcgtg gatcaccaga cgatcgccgt ccatacgggt
    55681 aaagcagata tgtgtttcag tcgggcactg ctgtgcctgc gttgagttat aggttcgctc
    55741 aatgatcaca tcggcatcgg caaagccttt gtccatatca ccaatatgac catgaatact
    55801 ggcagcgata tttttgcgtg ggcgagaacc gatcgggaag ttgatgatca tatgctcgcc
    55861 gcgctgggct gcatgacggt tatcttcctc cagagtatct ggtgcaccag caacatacac
    55921 caccggttca tcgtgcacga caggcgcatc ttccgccatt gcctcgtcaa tcgacattac
    55981 cggcttaagc acttcatatt caacgtcgat gagcttcagt gcttcgagcg caatgtcttc
    56041 actttcagcg acgaccgcag caacgcgatc gccgacgtga cgcattttct tgccgaacat
    56101 tcggcggtca agcggtgacg gttccggtgc gctctgacca cccggggtat agtaaatatc
    56161 cgggcaattc aggtgagtaa taacgtgaac gacgcccggt aaggcttcag ctttgctgac
    56221 atccagatga gtaatcagtg cgtgagcgtg tgggctacgt aacattttaa tgacgcaggc
    56281 gtcagccgtt acgcgatctt caacatagca gggtttcgcc tgcaccattt tcgcggcatc
    56341 agttttagga taatgcttgc caatgacttc aaggtcgtca cggaaagtcg gagcgatatc
    56401 aatggtggcc tgcggattat ttttacgtgc aaccgccagt tcaatgacct gatagtattg
    56461 ctgccagcca gcatcgcggc taaacagacc tgagagcgcg tcgtcgatct cttcgcgggt
    56521 aggtgcgtcg atgcgatcga gaagatcggt gataatcaga gccgcagctg gatcgttata
    56581 accagactgc accacgccaa catcaaccat ggcctgttga accagactta actcattcca
    56641 tttgcccaga gattctgcgg tacgaatatc tgccttctct aactgtgcgg cgataagcaa
    56701 ggacgcgtta acgatattac cgttaaagat aattgcgtca gaaccggcaa acccaaaacc
    56761 atcatcactg ttgcgtacag agtgcattcc catgttaaac aacagctttt gcacgttttc
    56821 gcttggatta acggttagct cctgaggcgc gccatttaaa gtaaagtgga tgatcatacg
    56881 gcttcctccc cagcctgttg gcagtcggca tacaggtcgg ctaccagaac tcccgtgata
    56941 tagcgtttat aggccacgct gccccgcaaa tcttcctgcg ggaaaatggc gttggcgaca
    57001 gcctgttcaa gtgcattgcc ttccagattt tgagtttcaa catcatgcag acgcagtgct
    57061 ttactggcta cgccatccag cgcaattcgc ataccgtcgt ggtctgtcat tgcaacggct
    57121 gccgtcacga cggttaaacc tgcctgagaa cggctaattt tgcgggtcgc acaggtgcga
    57181 tacggatctt taatgataat ttcggttaac aggcgatcgc atgggcaggc caggtagtcc
    57241 tcgattgaca gcgtttcgcc gttgccaaaa accagttcag catccagtgc cagcaggacg
    57301 ggaagcagca ccgactcttc ctgccgggcg gcgatttcac caccaatggt cgactgatta
    57361 cgaacatggc gtgagtaaac aaaaccgagg gcttcacgca gtgctgcagg aataaatcgc
    57421 gcatcacgca gtggctgcaa gcgagacatt gcaccaatcc gcagtgcacc gttatcccag
    57481 tcaacccagt ccagttccag atcctgtaag gaaatggcaa tctttttatc ggtacgggtt
    57541 ggtgtagcat tgagtttgct gcccccggcg aaccagacgg cttcatcctg gtagcggcgc
    57601 ttcagttcca gcgcctgttc gacagagtcg ggcctgaaaa attgttcaat catcttcttt
    57661 ccttatttaa ccctcttccc tgcgggaaga gggtatctgt catttatgcc agcgcatcca
    57721 tccgacgcca catactggca gcggcttttc tggcttgcgc ataaatggaa tcgcaatcga
    57781 agttaaactg acggtcttca tagaccatca caccattgac catcacgctg tgaacgctgc
    57841 ctgagcccat accgaaagcg atatgcccgg cgatattgtc tgccaacagc ggcgtcggcg
    57901 agttgtaatc gcaaatggtt aaatcagctt tgtaaccggc ttccagaagc ccaaatttcg
    57961 cgccaaagtt gcggctcatc agttcgttac cgttagtcag ggctttggca aaactgccag
    58021 gccacagcgg accacccgca tcgcgatgtt taaagaaggc aaatttcatc tcttcaaaca
    58081 tgtccgaacc aatgccgtcc gttcccaacg ccagattgcg gatgtcgcta aggtgatggt
    58141 tgtagccgac atggttgttc atgtttgaac gggcgttatg caccaggaac gcatcgcgct
    58201 gattgagtag ggcgatgtca tctttcgaca agtacagccc atgagcgacc agcgttttgc
    58261 tgtcgatgag atcgaattgc gccagtcgtg ccagcaggtc tttgccgtac cagtggtgac
    58321 tgtaggaaac gtcataaaga tcttccgcag cgtgaatgtg caaaccacga cctgtggctt
    58381 tcacggcttc acgcagcatc tccagaccgg catccggcac ggtaaacggc gcgtgagcgc
    58441 cgatatgagc ttccaccaga tacggctcgg ttgctgcttt cttcgcctca tcaatctgac
    58501 gggcgaagcg gatgttttct tctacacctt cctgcaactc tttgatgccg ttgttacggt
    58561 cagtagtttc aaaacaggtc atcgcgcgca ggccaacttt taaaaatgcg ttgcgcaatg
    58621 tggagagcga cccgccgata tacgccggag aggcatggtg atcgataact gatgtacatc
    58681 cgctcttaat cgcttccagg gaacaaatca gtccgctgta atagagcgac tcttcatcaa
    58741 gggcgcgatc gagccgccac cagagatttt tcagcgttga gatgaaatcc gggcaggggg
    58801 cgatgtttgc cataattccg cgggaaagtc ccgagtaaaa atggttatgc gagcagacaa
    58861 ttcccggcat cacaatccga ccatgcatct ctttgtagct ggcatcgggg tagcgttgtg
    58921 tcagggcatc gccgatagcg acaatcacat cattttcgat agcgatatca acgccttcct
    58981 gcactttcgc cgggtgtaac tgcactgcgg tgacattctt cagaatcaac atgattacac
    59041 ctccacgcgg cccagcagat aatgatgatg ctggtggaca tggctaataa tgcggcacat
    59101 atcgttcagc tccggtggta cgttgttgaa ctgaccttcg ctgtcgatgt ttaacaccca
    59161 gctttggtta ttcagacgta ctcgtacccg gcaatcttcc acaaggaagc cagggttgct
    59221 gctgttatca aagtcttgcg acaggctgaa gacggtgatt ttgtctttgt atggtttacc
    59281 gttccacggg cagaactgag cgcagttgcc gcattcgtta cagtaagcgt cgaggtgcag
    59341 cgtctggaaa cggttctgga agcctgggac cgcaatggag acgttggcgc ggttcgggca
    59401 gacatccaca cacttgctgc aaacgtagtt acattcaagg cagcgtgcgg cttcctgggc
    59461 gacaaacgcg tcacgatcgt cgctatccac cagggtgaca gagatatcgc ctttgcgttg
    59521 atagatttcc gccggattaa cgttgttcca gtatttatcg ttctggtggg aacggatatt
    59581 ttcccgacta aggatggcat cggtcgcccg acgcgcggtt ccgacagcag cgacaatgga
    59641 ggatggtccg cgctgtacgt cgccgatcat aaagacgtca gtcagacgag tttcgccatt
    59701 atggtcgacg tctggccagc cgtttttgtc cagcggcacg cccattgcat tcagggcttc
    59761 agtatcctgc tgttcaccaa tggcggtgat caggctgtct acatgcagcg ttactgtttc
    59821 gttggtttca accggacggc gacgaccttt ctcatcaggt tcgccaagcg acataacgcg
    59881 caaggttaag gtgccatcag catcgaaacg ttccggatta ttcagaaaac ggaactccac
    59941 gccgtcgtgc aacgcttctt catactcttc gcgccatgcg ggcatttctt gcagtgaacg
    60001 acggtaaacg accgttgctt tttctacgcc tggaacgcgt aacgccgcac gagcgcagtc
    60061 cattgcggtg ttacccgccc cgacaacgac cacatgtttg cccagcttga gcgcggtgcc
    60121 cttgttgtat tcacgcagga aagggagtga tttccagaca tttgggttgt cgcccgccag
    60181 tttcacaccg ctatttttat cagtgccggt ggcaatcaga acatagtgga agccctgatt
    60241 ttttaactgt tcaatggtca aatcgggtga gcaaccgtac tcaaatttca cgccgtgatc
    60301 ggcaacaaaa tcgatatcgt gctgaattaa ctctgcagga atacggaact gaggaatgat
    60361 atttttcacc acgccgcccg cattggcttc gcgttcaaac agcgtaaccg gatggcccgc
    60421 tctggcaagg aagtaacctg ccgccagacc tgccggacct gcaccaatca cggctaccgg
    60481 atggcgtgaa ccggaaccgg ctggtttgtg ccagcgttgc ttatattcat cccaaccttt
    60541 ttccagggcg acttttttca gttcgcggat attcagcgca ctgtcgtaat ccaggcgggt
    60601 acagttgtat tggcactggt gatcgcaaat atgaccggta atggcgggca gggcgttgcg
    60661 ttgatagatg agttccagcg cgtcggcata gcggtgttcg ccaagcagac ggatgtattc
    60721 cggaatatct tgcttgatag cgcaggcagt aacacagggg gcaacgtagc aatcggtcag
    60781 cggcaggtct tcagccactt caatacgctc ttctggcttc cagtgtttct gggtgtattc
    60841 catggttaac gcgtctgctg ccagtctgtt cagtcgttcg acgtcaacgt ggttaagctc
    60901 ccaggcatct gagccttcca gctcgcgcat gcaggcactt aagcgcagat agccgccagg
    60961 tttcagcagg tcggttgcca tagtaatagg gcgaatacca gtatcaaaaa tatcgcggat
    61021 agtcagctga ctggcaccac cggaataaga aatgggcagt ttgccgtcaa aggcgcgaga
    61081 gagaactgct gcaacgttga tggagagcgg gaacagcgca cggcctgaca tatacatctc
    61141 ttcaccgggc agtgcgcctt tattgttgat ggtgccgaga gtgttagtca gttttacgcc
    61201 aaagccgagt gatttttctt ttgccagtgc catcaggcgt tccagcattt ccagtgcttg
    61261 cgtcagcttg aggtcgtgat caaatgactc ttcttttaag ccgatgtaac cgaaaccgca
    61321 gacatcgagg atctcacgaa cacgcgcgta ccccagtaag gtcgggttga gtttcacaaa
    61381 ggtgttgagc cctttttctt ccagcatgta gcggcaaatg gcttcgattt catgcggagg
    61441 acagccgtgc atggtggaga gggtaacccc ttgtaccatg ctggtgggga tgcgagcggg
    61501 taaggcttgc aagctttcgc gtttttcctg caatccgtgg cgggctaaaa atgcctcatc
    61561 ctggagtagc ttattaagtg catcgcggta ttgagcgaat ttcggatggt cagatgcgtc
    61621 catcatattg tcgatgaact gctgcatcgg cggttgctta ataccttcga ggttgtaacc
    61681 gacgctcata ttaaagatga acgatttacc ggaatcagaa ggctggaaca tcgcttcgag
    61741 aaggtgcagg gcaaaccagg ctttgaggta ttcatcccag gctttaagca gggtaaattc
    61801 ggtagaccat tcggtgttaa agcactcgtc ttcggcatcg atacagggct tttccagctc
    61861 caggcggtca agaatttgga cggtttttag ttcgatgaag cgtccgccag tcagccagga
    61921 ggtgacgatg ttttgcgcaa gttgcgtgtg cgggccagcg gcagggccga cgggagtggc
    61981 acaggtttca ccgaagacgc taacagtttt accttttacg ggtgagtaaa actgttgctc
    62041 gggaatacca aagattgagc gttgttgttg gtattcatca aatatgcgcg tcaaaagttc
    62101 ctcaaacgga atgggacgca taatatcccc cataaccctc tccttagctt atacagatat
    62161 cattgagtga ccggtgttga gaaatccggt ggtttagggg gatagagcaa caatcgcacc
    62221 actcttgcca ggtttggcgg gaaatataaa aaaatgtgag gcggctcgaa acttaatggc
    62281 tttttgtgaa tgaactcaca ggtgccaaat gagaattaaa atcactcctg aaaattatta
    62341 ttgccagccc atttatcact ttttctcttt tctcaaaatc agtgattcct tatcattatg
    62401 ataaagagtg ctggagggat tagatatttg aacgcaatcg tttaactacg ttttctaaat
    62461 ttcagtatac cttttcttcg ctgtaataaa gtcgtcagga gtatcaatat ctaaaattat
    62521 ttctgcattt tctatttcga cggaataatg ttctcccata aggagtgcct gacgcatatt
    62581 ggtgacattg ggtcgctgga ttgcctgcat caggcatggt tttgacacta aaatcggatg
    62641 gccggggatg ccattatgga gtggcagtat tgcgccatca tttcgtaacg accagatttt
    62701 tctaaaaata tcgatggtga gggttggcat atctccgtgg gtgagaaaac aatgttctgt
    62761 ttgcaccgcg ggtactgcgg cctttactga tgtcagtaaa ccctgcgcat aatctgggtt
    62821 gtggataata gtgatattgc tctggttcgc ataacgttcg tgcagttcat taccacgata
    62881 gccggtgact aaaataattc ggctacaaaa ctgcaacgca tttttgatac ttgtatcaag
    62941 aattgttccc tgttgccagg gtaacatcat tttccattgc cccattcttg atgataatcc
    63001 ggcagcggta attatacagt cgatggctga catagtgatt ccattattta caggagctaa
    63061 taacgcctga taattgcgtt acttcctgat gcattaagtt ggggataatt tatcccagag
    63121 aggtccataa agacgcgtga aaagtataat tgacccatcg gcattattca ttgatttagg
    63181 cgcgcagaaa cgtcccactg tgatttctat tgttggtgct ggggggaaaa ccagcttgct
    63241 tttttggctg gcagaactgc tccaggctag cggcaggcgt gtgttaatca ctacgacgac
    63301 acatatgttt atgccaacgt ctcactggcc cgtggttttc tgtcgtgatc ccgccatgct
    63361 tcctcatgcg tctcttacat cccccatttc attttgtttt cacagctgga aagcgaacca
    63421 gggaaaagtg cagggattta cgccagaagc gattgatgca ctggtgcaac gaccagagtg
    63481 tgacgtaatt ctcattgagg cagatggctc gcgtggaatg ccgttaaaag cgcctgatga
    63541 gcacgaacct tgcataccca aaagcagttg ctgcgtgatt gctgtgatgg gaggacatat
    63601 tttgggcgcg aaagtgagca cagaaaatgt ccatcgctgg tcgcagtttg ctgatattac
    63661 cgggttaaca cctgatgcaa ccttgcaact gagcgatctc gttgcgctgg ttcgccaccc
    63721 tcagggcgca tttaaaaacg taccgcaagg ttgtcggcga gtctggttca ttaaccgttt
    63781 ttctcaatgt gagaatgcga ttgcgcaaag cgagctcctt caaccgctgc aacaacacga
    63841 cgtagaggca atctggctgg gcgatataca agaacatcct gcaatcgcgc gcagatttgt
    63901 gaattagcgg cccgggtatt ccgggttact gaaaggatat ccattgaggt tggtatgaat
    63961 attttcacag aggctgcaaa acttgaagag caaaattgtc cgtttgcgat ggcgcaaatt
    64021 gttgatagcc gaggttcgac tcctcgccat tctgcacaaa tgttagtgcg cgccgatggt
    64081 tctatcgtcg gtacaattgg tggtggaatg gttgaacgga aggtgattga agagtcgctt
    64141 caggcattgc aggaacgtaa gccgcgatta ttccatggac gtatggctcg taacggtgcg
    64201 gatgctgtcg ggtcagattg tggaggtgca atgtcagtat ttatcagcgt ccatggtatg
    64261 cgtccacgtc tggtgttgat tggtgcgggg catgtcaacc gggcgatagc ccagagtgcg
    64321 gcgctattag gatttgatat cgccgttgct gatatttatc gcgaaagcct caatcctgaa
    64381 ctattcccac catcaaccac gcttctccat gctgagtcgt ttggtgcggc agtggaagca
    64441 ctggatattc gccctgataa ttttgtcctg attgccacga ataatcagga tcgtgaagcc
    64501 ctcgacaaac tcattgaaca acccattgca tggctggggt tgctggcaag tcgccgcaag
    64561 gttcagcttt tcctgcgcca attgcgtgag aaaggcgtgg ctgaagaaca tattgcccgt
    64621 ttacatgcgc ccgttggtta caacataggt gcggaaacgc cgcaggagat cgccattagc
    64681 gtgctggcag aaatattaca ggtgaaaaat aacgcgccgg gtgggctgat gatgaagcct
    64741 tctcatcctt ccggacacca gctggtggtg attcgcggtg cgggggatat cgccagtggt
    64801 gtagcgctac gtctgtatca tgcgggtttt aaagtgatca tgttggaagt ggaaaaaccg
    64861 acagtgattc gttgtaccgt ggcgtttgcc caggccgtgt tcgatggcga aatgacggtc
    64921 gaaggcgtca ctgcccgcct ggcaaccagc tctgcggaag cgatgaaact taccgaacgc
    64981 ggattcatcc ctgtgatggt agatcccgcc tgttcattgc ttgatgaact gaaaccgctt
    65041 tgcgtggtgg acgctattct ggcgaaacag aatttgggga cgcgggcaga tatggcacca
    65101 gtaacaatcg cgcttgggcc gggctttatt gcagggaagg attgtcatgc ggtaattgaa
    65161 acaaatcgcg ggcactggct cggtcaggtg atttactctg gttgtgcgca ggagaatacc
    65221 ggtgttcctg gcaatattat ggggcatacc acccgacggg tgatccgtgc tcctgctgca
    65281 ggcattatgc gatccaacgt gaaattaggc gatctggtga aagagggcga tgtgattgcc
    65341 tggattggtg agcatgaaat taaagcaccg ttgacgggga tggtgcgtgg cttgttgaac
    65401 gacggactag cagtggtcgg tggttttaaa attggtgata tcgatcctcg tggtgaaacg
    65461 gctgatttca ccagcgtttc tgataaagcc cgggcgattg gcggcggcgt acttgaggcg
    65521 ttaatgatgt tgatgcatca gggcgtgaaa gcgacaaaag aagtgctgga agtggcttaa
    65581 atgaaaatgg ccggatggta ttacccaccc ggcctttaaa ttactgacaa atcaccgttc
    65641 cggttttgcc ttcaatccct tctttcgctt tgctcaatac ggtaatcagt gcttcacgac
    65701 ctgcgcgaga gcgagcaaac gaggcggcag cttccacttt aggcagcata gaacctttcg
    65761 caaaatgacc ttcttcgata aagcgttcag catcacttaa tgacaggcga tcgagccact
    65821 gttcattctc tttaccgaag ttaatggcca ctttttctac cgcagtcaga atgatcagca
    65881 tatcggcatc aatcatttct gctaaacggg cgctggccca gtctttatcg ataaccgcac
    65941 tggcaccacg cagatggtta ccttcacgaa taacaggaat accaccaccg ccaacggtga
    66001 tcaccacctg acctgcatct accagagctt taaccgtttc tttttcaatg atatcaaccg
    66061 gttttggcga ggcaacaaca cggcgatagc cgcgaccagc atcttctttc agagtgtaac
    66121 cctgttttgt cagttgctct gcttcctgct cggtaaagaa cgaaccgatc ggcttggtcg
    66181 ggttgaggaa tgccggatcg ttagcatcga cttcgacctg ggtaaccagc gttgctacag
    66241 gtttattaat gccacgagaa agcagttctt ctcttaaggc gttttgtaga tcgtaaccaa
    66301 tatagccctg gcttaaggca acacagacag acatcggcag cattggcgag tgcgcttcag
    66361 tttttgcggc agcttcaaag gcctggttaa tcatgcccac ctgtggcccg ttaccgtgag
    66421 tgacgataac ttcatgtccc tgggcaatta aatcaacaat tgcctgagaa gtaattttta
    66481 ccgctttcat ttgtccggcc aggtcatcgc ccagcgcatt cccgcccagg gcgagaacaa
    66541 ttttcttact catttttcct cactcatcat tcttgattgt gtatagatcc ctggcagtgg
    66601 ttagagcacg ggagggacaa acggtttgcg tcgcaggaaa cgacctcgcc cggctttgcc
    66661 tgtaaaggtg ccgtcacaga aaatcgtttc accacgggat aatgttctga caattgcgcc
    66721 ctgacaggta aacccctccc agggcgagta gtcggcgttg tcgtggagat ggcgatgctg
    66781 aatttgttgg ctctgacgtg ggtcgataat caccacgtca ccatcggaac cgggcgctaa
    66841 tatccctttt tgcggccaca ggccaaacag cctggcgggc attgcgctgg ttagttcaac
    66901 aaaacgttcc ggtgttatac gtcccgtcat cacgccactg gaaaacagta actgcatgcg
    66961 gttttccaca ccgggtaagc catttgggca gcgactgaaa tcgcctttag aaatttgcag
    67021 gcgctgggcc atcgagaagg tgcagtgatc ggtcgccacc acgtcaatcg caccatcgct
    67081 gatgccacac cacagtttgt cctgctcgcg tacgttacgc agcggtgggc taagaatgaa
    67141 cttcatgcca tcttctgtat cgtaactgcg ttcgtccaac aggagatatt gtgggcaggt
    67201 ttcaacccag actggctggt gattcgcacg ggcaagacgc agataatcca gacctaagcc
    67261 gttagacaga tgcacgatat agagcggggc gttaccggca atttgtgcca ggttaatcat
    67321 gcgggcgatg gcttccgctt cgcattccag agggcgactc aaggcgtgat agcgcggcgc
    67381 ggttaaccct gcggcgataa attccgcccg cttgctggcg atagccgcat cattttccgg
    67441 gtgcacggtg gtcagcgcgc cggattcatg cagacggcgt aatgcctgca aaacctcgtc
    67501 atcgttgagt ttgtattgat aggttaaata gagtttaaaa ctgctcagtc cttcttcgac
    67561 catcatcgga atttcgtcga ggatggcgtg attaatgtgc tggatcacac catgaaagct
    67621 gtagtcgatg accgccttat gggcggcata gccacgataa acctccagtt gatggcgtaa
    67681 ccggcagcca ttcgggccaa atcccatatg gtcaataatg gttgttgtac cgccacacgc
    67741 agctgcgcgg gtaccggtaa aaaaatcatc acaactgcgc gcgatgccga catctatatt
    67801 gaaatgcgta tggacatcca cgccgccagg gaaaacgtaa cagccagtgg catcaatttc
    67861 ttcatacggg agctgcggcg aaatattgct gcccaattgg cgaacaatcc cgctctcaat
    67921 cagcaaatct tgtttggctt gtccatctgc gttaacgaca gtgccgtttt tgatcaatac
    67981 gcgcatagca aactccaaaa accctccggc gaaccggagg ggaataagtg aagagtaatt
    68041 attcggttgc cagccagctt aacgggattg cagcgtacat cgcggcacag gtcaccaggt
    68101 gagatttcca ggttttttcg ttaggtgcat gcgcttcagg ttctttaccc gggccaaagc
    68161 cgatcaccgg aatgccgtga cggcccatga tagatacgcc gttagttgag aaggtccact
    68221 tatcaacaac cggcgctttg ccaaacagac cttcgtaggc attcaccagt gctttaacag
    68281 tgaagtgatc ttcttccact ttccaggtcg ggaagtagca ttcggttggg taaaccaggc
    68341 cagtccagga cggacggtcg tagttgtaca tagaaacaac cgcgttagct ttctgtaccg
    68401 caggcagggc gcggatttcg tccagcgcgc cttcccaggt ttcgccccag gtcagacggc
    68461 ggtcgattga aactgcgcag ctatctgcta cagcgcaacg gcttggggat gtgaagaaga
    68521 tttcagaaac ggtgagggtg cctttgccga ggaattcatc atagcccagg cgctgggaga
    68581 gttcttgtaa ttcgccaaga atcggaccca ttttgaaaat ggcgttgtca ccgcgttctg
    68641 gtgcagaacc gtggcagcta acaccctgaa catcaatgcg aatttccata cgaccgcgtt
    68701 gaccacggta tacctggcaa tcagtcggtt cagtactgac cacaaattcc gggcgaatgc
    68761 cggattgttc aataatgtac tgccagcaca gaccgtcgca gtcttcttcc tgcaccgtac
    68821 cggtaaccag cagggtatat tcatcttcca gaccgaggtc tttaatgatt ttaccggcat
    68881 aaaccataga tgccatgccg ccttcctggt cggaagtacc gcgaccaccg atcagctcat
    68941 cagtttccat gccttcgtac ggatcgaagt cccagttttt gatgttgcca atgccgacgg
    69001 tatcaatatg agcgtccatt gccaccagac gcgggccgtg gccgatataa ccgagaacgt
    69061 tgcccatcgg gtcgatttca actttatcga agccgacttt ttccatctct tctttaatac
    69121 gatgtactac gcgtttttcg tcgcagcttt cactgggaat agcaaccata tcgcgcagga
    69181 agcgagtcat gtctgcctgg taatcttttg ctttttcaag aatcagtttg aatggaatat
    69241 tcttagccat tatttttctc cagatttttt actccccgtt ttgcggggag cagttccaat
    69301 agggtgatta aggtgctaca gcgtgtttgc cttcccagac aacttcgcgg tagtgcttca
    69361 catcggtgtc gccttcagtg ctgataacca gcaccacggc atctttgttc agcgccagtt
    69421 tttccatcag gctttgacgt tgcgggtgat aatgaaccgc tgcgagaacg cccaaaccga
    69481 cagcgccgga ttcaccggag atgatgcgtg gatcgttgcc gtacggatta cccagtacgc
    69541 gcatacctaa tgcggcaacg ctgtcctggc aggagatgaa ttgggtggcg cagttacgta
    69601 ggatttccca gcccagcggg ttaggttcgc cacaggccag gcctgccatg atggtggcca
    69661 tatcaccgcc aacgttgatg atgtcgcctt tgacgccaga gcgataaata cagtcagctt
    69721 tgtcaggttc aacaataatg ctgtgcagat tttgcgggct gtagacatcg accagataac
    69781 ccagcacacc accggccatc gcgccgacac cggcttgcag cagaacgtgc gtcggggtta
    69841 cgcccatttc acgcatttgc tcgacggctt catctgccag tgttgcatag ccctgcatga
    69901 tccaggttgg gattttggtg taaccttccc aggcggtatc ttgcaccact tcccagccgt
    69961 gctgctgcgc gtgttgcatg gtcaggcgaa cggtatcgtc atagttcata tccgtgacga
    70021 tgcactcggc accgaggttc agaatggcgt caacgcgttc ctgagcagaa cctttcggca
    70081 tgtaaatcac cgcattctgt ccgagttgct gtgctgccca cgccacaccg cgcccgtggt
    70141 tgccgtcggt ggtggtcgcg aaagtcattt tttcgccgat ggcatttttc aggtgctcaa
    70201 atgacagcgt ttcgatatca agatgatatt tttcgcacaa taattgagcg atggcgtatg
    70261 caccgccgag cattttgaag gcgttcagac cgaatcgttt tgattcgtct ttgacgagaa
    70321 ttttcttcac accaaaaagg tttgcgagat cgtccagcgc acaaagcggt gttgggcgat
    70381 aaccagcaat tttctggtgg aactggcgcg ccagtttggc ctggctttgc gaaaagagcg
    70441 gtgatgtttc gccgttgaaa aatttgttat cggcgatatc aatcttcaat gagaaaacgg
    70501 acatatccta tccttttggc ctctggaaat agagggccga ccagctgatc ggcccgaggg
    70561 gttatttcac gcgttcttgc gcttctttca gcagttgctc gagcagtgca cctggtttgg
    70621 cgtatttacg ggacaggatc atcgcggcga tgatataagg cttccagctg gcttctttgt
    70681 aggttgcgat acggtatttt tcgaatacgc cttcagtcac ttcaccttct ttgcaggata
    70741 cgccgctgat atcagctggc aggcagtgca tgtacagggc ttcgccatca cgggtcagtt
    70801 tcatcatctc ttcggtgcaa tgccagtctt tgtgctgagc gttctgcgcc agacactgtt
    70861 tttccagtgc ttttaagcct tcgtgatcgt tagcgcgcag caattcagta cgctcttcca
    70921 tcactttgta aggtgcccat gacttcggat aaacgatgtc tgcgtctttg aaggcttctt
    70981 ccatgctggt gacctgacgg aagctaccgc cagaggcttt agcattgttt ttagcgactt
    71041 caacgacatc cgggatcagg tcgtagcctt ccggatgggc cagggtgaca tccataccga
    71101 agcgagtcat cagaccgatg atgccttgtg gtacagatag cggtttgcca tagcttgggg
    71161 agtaggccca ggtcatggcg atttttttac ctttcaggtt ttccagtgaa ccaaagtgtt
    71221 cacgtaacca tgcgaggtca gccattgact gagtcgggtg gtcaatatcg cattgcaggt
    71281 tcactaaagc cggacgctgc ggcagtacgc cctgcttgta gccgtcatca agtgcagcgc
    71341 caacttcacg catataagcg ttgcctgcgc ccagatacat atcgtcacga ataccgatgg
    71401 catcggcgca gaaggagatc atattggcgg tttcacgcac ggtttcgccg tgagcgattt
    71461 gtgatttgcc ttcgtcgaga tcttgctgtg cgaggccgag caggttaagc gcggaagcat
    71521 aagagaagcg ggtacgggtg gagttgtcgc ggaatacgga aatacctaat ccactattaa
    71581 agactttggt tgagatgttt tcagcacgca gtgcttttaa tgctgcggca acgtccagta
    71641 cttgtttcag ttcatctggc gtctgttccc acgttaacaa aaaatctttc tcgtgaaggt
    71701 gagaggtcag cgaattgata tccttaatca gctcattaac agttttcatc ataaagtccc
    71761 ggtagtggaa tgacaggcaa agaggtttat ttcactgtga cttgccaaaa agtgattggt
    71821 cttttcacag ctatacctgg gactaacaat aactatgcca gaattatcca agcggctgca
    71881 aaagactaat ttgacgggta atgtgtgggg ttgatcacaa attgatgcca gcttctggtt
    71941 gtttgtaaaa tacgaaaacg tttggctttt tttatcgcgt tatttatttg ctgctaagta
    72001 tcagaaataa gataaagggg caggttatcc ctcgtcagtt tgataaactc accgccgtaa
    72061 ttctcatatt gatatagaaa tgtgatatct cttccataat ttcattagaa agtgataaaa
    72121 atcttatgtt tttatcaaag ccaatttcgt ctgagttaat atcatcatga tctgtgaacc
    72181 atcaacgtct tcaatgatcg acttttaagt aagtctggaa ggtaaagcta tggagcttgc
    72241 tactacgcag tcagtactga tgcaaattca accgacaatt cagcgttttg ccagaatgct
    72301 tgccagcgtt ttgcagcttg aggttgagat cgttgatgaa aacttgtgtc gcgtcgccgg
    72361 aacgggcgcg tatgggaagt ttcttggtcg ccagttgagc ggcaactcac gcctgctccg
    72421 ccacgtcctg gaaacgaaaa ctgaaaaagt tgtgacacaa tctcgcttcg atcccctttg
    72481 cgaaggttgc gatagtaaag aaaattgccg tgaaaaagca tttctgggta cgcctgtcat
    72541 tttacaggat cgttgtgttg gggtgataag tttgattgcc gttacccacg agcaacaaga
    72601 acatatcagt gataatttac gcgaattttc tgattacgtt cgccatatat ccaccatttt
    72661 tgtttcgaaa cttctggagg atcaggggcc aggagataac atcagtaaaa tattcgcgac
    72721 catgatcgat aatatggatc agggcgtttt agttgttgat gacgaaaatc gggttcagtt
    72781 tgttaatcag actgccttaa aaacactcgg tgttgtgcaa aataatatta ttgggaaacc
    72841 tatccgtttc agaccattaa catttgagag taattttacc catggacata tgcagcatat
    72901 tgtttcgtgg gacgataaaa gtgaattaat cattggtcaa ttgcataaca ttcagggccg
    72961 acaattattt ttaatggcgt ttcaccaatc gcataccagt ttttctgtag caaatgcacc
    73021 tgatgaaccg catattgaac aattggttgg cgagtgccgt gttatgcggc aattaaaacg
    73081 actcattagc cgtattgcac ccagcccatc cagcgttatg gtggttggcg aaagcggcac
    73141 gggtaaagaa gtcgtcgccc gtgcaatcca taagttgagc ggaagacgga ataaaccttt
    73201 tattgctatc aactgtgccg cgattccgga gcagcttctg gagagcgaac tgttcggtta
    73261 tgttaaaggc gcatttactg gcgcatctgc caacggtaaa acagggttga ttcaggcggc
    73321 gaatacgggc acgctgtttc tcgatgaaat aggtgatatg ccattgatgt tgcaggctaa
    73381 attactgcgc gctattgagg cacgtgaaat tctgccgatt ggtgccagta gcccaataca
    73441 agtcgacatt cgcatcattt ctgcaactaa ccagaatttg gcccagttca ttgccgaagg
    73501 taaattccgc gaagatctct tctaccgact taatgttatc ccgataactc tgccaccgct
    73561 gcgtgaacgt caggaagata ttgaactact ggtgcattac tttttacatc tgcatacccg
    73621 tcgtctggga tcggtttatc cgggcattgc tcccgatgtc gtcgaaatat tgcgtaagca
    73681 tcgttggccc ggaaacctgc gcgagttaag caatttgatg gaatatctgg ttaacgtggt
    73741 tccttcaggt gaagttatcg acagcacgct attgccgcca aatctgctga ataatggcac
    73801 aacggagcaa agtgatgtaa cagaggtcag tgaggcgcac ctggcactcg atgatgcggg
    73861 cggcacggcg ctggaggaga tggaaaagca aatgatccgc gaggcgcttt cacgtcataa
    73921 cagcaagaag gaagttgctg atgaactggg catcggcatt gctacgctct atcgcaagat
    73981 taagaaatat gagttgttaa acacataacc tcagtgaatc cggcctgaat tcaggccgga
    74041 tatcctttta cttcgttttc tcgcaatcca gaactgtatt tacaatcatc tgataccccg
    74101 tgcagcgaca aagatttccc gccagtccac gacgaatttc cgtaatggtt aatggcttct
    74161 cgcgtggttt cgccagcatt gccgtggtag ccataatcag gcccggcgta caaaacccgc
    74221 actgcactgc gccggatttc gcataagcct gctgaacatg agaaagtttt ccgcctttcg
    74281 cttcaccttc cagcgtgcgg atctcttttc cttcagccca ggcggcaagg tataagcaac
    74341 tgtctattgc tgtgccgtcg accagcaccg tacaggcacc acattcaccc acgcagcacc
    74401 cttgtttgac acttagcagt ccttgttcgc ggagtaattc cgagagcggc gtgcctggtg
    74461 cggcgtgaag ctgaaaaggt atcccgttaa tggtgcattc gatggtaatt gtttcgctgt
    74521 gattcattgc aattttcccc ccgccgcggc gacggcttcg ctaatcactt ttttggtcat
    74581 cgtctggatg agatgcagac gaaactcttt actggcccgc catgacgaac gcggggcgac
    74641 atcttgcagg actgattcgc tgatggcttc cagcgtttgc aggtttaatg gcgcattttg
    74701 tgcagtctgt tcggcatgtt ggcagcgaat cggcgttggc gcggcaacac caaatgccag
    74761 acgtaattcg ctgaaattgc cgttatccag tcggcaatgt gcggcgcagc caatcgttga
    74821 aatatccatt gcgtcgcgca tggcatattt aaaatgcgcg ctgcccgcgt gttctttcgg
    74881 ctggggcgga aaatgaaagg cgacaaggat ttcgtcatgc tcaagagaca ctttgcccgg
    74941 cccggtgtga aagccattaa tcgggacgaa acgaacaccg cgcggggagt ggatctccag
    75001 tttcgcgtca taaattagcg ttggcgtggc agaatctgcg ctggtggcac cgttgcaaat
    75061 atttccaccg taggtagcga cgttacggat ctgcggtccg gcaatggatg aggccgcagc
    75121 acataacgcc gggagatgac gttgagttac aggatcttca atgagctggg taaatgtcgt
    75181 tgcagagccg atacgtagtg agccatcttc cgccagcgta attccccgca gctccgccag
    75241 attatgaata tcaacaatat ggcgataacg gtcattgtga tggtggagct gaatcagtac
    75301 gtcagtgcca ccggcgagca gtttggcctg cgggttgtca gccagcaggt tgatggcatc
    75361 ggcaagggtt gctgcgcgat ggtaagaagc aaaatcaaac atgatgttat cctcaaatca
    75421 atcctgccag atggaactct tcatataacc gttttggcgt cagcggcagt gtattgattg
    75481 caacaccggt agccatcttc accgcgttac gaatagcagc ggcaacagga attattggtg
    75541 gctcacccag tgacttatgt ccgtatgcgg attgcggctc attgatttcg acgaacgcgc
    75601 tttccagttg tggcagatcc ggcatggtcg gcattttgta atccagcaga ttggggttac
    75661 ggaccacgcc gcttttcgca tcaatgatca tctcttcaaa tagcgcccag ccaatgccca
    75721 ttcccattcc gccgtgtacc tgaccttctg ccagtagcgg attaagaata tggcccgaat
    75781 catgaacgtt gaggatgcgg ttgatggtga ctttacacag cgcaatatcg accgtcagat
    75841 caacaaaggt acagccaaac gctggtgggt tagtggtggt tttgatggag ctttcggcag
    75901 agagctgccc gccgcgttca gggtggtaga aggcgtccat cgccaaatct tttaacgaca
    75961 ttaatggctc ttccggtcgt tcaaccagca cgatatggcc ttttatcagg gtaagattca
    76021 tcgctgactg atgtagcatg acagcggcat gagcgatgat tttctccttt aataacagtg
    76081 ccgcactgcg cagcgcaggc gcggcaacat agctctggcg tgaggcaaat gcaccgggat
    76141 cgaacggcgt aacgtcggta tcttgggttg aaataacgcg aacgtcgctg accggaaccc
    76201 ccacggtttc tgctaccatt tgcgagaaga cggtgtcggc cccctgaccg atttctgtcg
    76261 cgccgctttg cacgttgatg gttccatcct gattcatcaa caggcgcgcg cctgctattt
    76321 ctacgccgac aggccaggtg ttagaggtgt agctaaaaca ggcgacgcca acgccgcggc
    76381 gtaaattgcc ttgctggttc tggcattctg cacggcgttt ttcccattca aagattttcc
    76441 ggcctttttc aagacactcc ggcaaccctg cgctgtaaat acgtttgccc gtgagcggat
    76501 tagcatctcc ttcgcgggcg gcgttgcgta aacgaatttc aacaggatca atacctaacg
    76561 ctgtcgcggc gtcatcaagc atagactcaa cggcaaatac gacttgtggc gcgccataac
    76621 cacgcatcgc accagccgag gggaggttgg tatagcaggt ctttgaactg taagcgtagg
    76681 cacaacgagg ataaaggtaa gcgactttat ttccgcccgc agaagcgatg gagtgcccgt
    76741 gagatgcata agcgccggtg ttagacagaa catccagact ataacctttc aatgttccgt
    76801 cgcggttcac gcccatttgc ccgtcaatag taaaagcgtg gcgggtacgg gttgcgagga
    76861 aacactcttc acggctaagg gaaactttca ccggaatgcc gccaagcttg ctggtcagga
    76921 atgccgccat tggctcttcc agtacatcct gtttattacc aaaaccgcca ccgacaaacg
    76981 gtttgatgac tcgtacgcat gaccagggaa tattcagcgc ctgaccaacc acacggcgaa
    77041 caatgtgcgg gatctgggtg ctggaaacga tggtaattcg cgagtcatcc tccatccatg
    77101 ccagcgatgt cacgctttcc atatgacaat gttgaataac gggagtctga tagtgcccct
    77161 gcacctggta atcggcggca tcgattgttt gttggacatt acccgtcgac atcgagcttt
    77221 gttttagtaa attgccgccg ttatggattg gtgcagcgtc atctgccagc gccgcttctg
    77281 gcgtggtgat aacgggtaat tcttgccact caatgctgac caattgcgcc gctttttctg
    77341 ccgtgagttc atcacgggcc acgacgatgg caacggcgtc accatgatga cgaacatggc
    77401 gagttagcag tgcgcgatcg gcggtatcgc gcttgttttc gtcaagtgtc catgcatgcc
    77461 cagctgtagc gaatgggata tcaggtacat cttcccaggt aaaaatcgcc agtacgcctg
    77521 gtaaacttct ggcttgttca tcattaatac ctacggcata accatgtgcg atagggctac
    77581 gtacatattt cgcgtaacac atgcccgcca taacataatc gtcagtatat cgtgcccgcc
    77641 cggtgacctt agcaatggca tctacgcgca tgcatgattc acctgtagcg gttgcttccc
    77701 gcgcttccat agaaatcccc tcgatttgaa catttggttc ttatggcaaa gatttagtct
    77761 aagcaggcaa gatttacgcc agaaacgacg ataagcgtta agggaggaag gaagtgtgat
    77821 gatgttaaaa aaacacagat atgatacgaa acaggactct gaatagggac agtgatacgt
    77881 atcaatatga gtggcgataa aatcaatgtg ataaatcagg attattacgc atcaaggtgg
    77941 gaaatagggt aaaagtcagg aacttcgata ccactacagc aaatattttg ctgatgatac
    78001 ttccctttgc caaatgaaat taacctcttt ctctattaca tcgtttttcc agaagcaaga
    78061 gattgggtat caagaggctg gctgctatga tatggcgtct tgttttttaa gcgaggaaag
    78121 attttgagtg cgggacgcct gaataaaaaa tctctgggta tcgtgatgtt gttatcggtt
    78181 ggactgcttt tagcgggctg ttcgggtagc aaatcatccg atacaggaac gtattccggc
    78241 tccgtttaca ccgtgaaacg gggggatacg ctatatcgta tttcgcgcac cacgggaacc
    78301 agcgtaaaag agctggcgcg actgaacggc atttcccccc cttacaccat tgaagttggt
    78361 cagaaactaa aactgggtgg ggcgaaaagt agcagtacac gtaaatcaac cgccaaatca
    78421 acgaccaaaa ccgcatcggt tacaccgtca tcagcagtac cgaaatcttc ctggccgcca
    78481 gtagggcaac gttgttggtt atggccaacg acagggaaag ttatcatgcc gtattcgaca
    78541 gctgatggcg gcaataaagg gattgatatc tcagctccac ggggtacacc tatttacgcc
    78601 gcgggtgcag gaaaggtggt gtatgtgggc aaccagctgc gtggctacgg taatctcatc
    78661 atgattaaac acagtgaaga ttacattacg gcttacgccc ataatgacac gatgctggta
    78721 aataatgggc aaagcgtgaa ggctgggcaa aaaatcgcca ctatggggag cactgatgcg
    78781 gcatctgttc gcctgcattt ccagattcgt taccgtgcta cggcaattga tccgctgcgt
    78841 tacttgccgc ctcagggcag caagccaaaa tgctgatggc gaattaatca gcagtcagca
    78901 gcgtggtact tgctaaggag agcgtaaggt ttataatgcc ttacgcatct cgaagcgggc
    78961 gtagttcaat ggtagaacga gagcttccca agctctatac gagggttcga ttcccttcgc
    79021 ccgctccaat ttatcttcac atcatctacc tgccattttt gttgtatctg attttctata
    79081 ttatcttaat tcaagcgctt cagacttttc gcaatcgccg taatacttcc ccattttttt
    79141 gtaaacaaaa agcgtttacg caatgcaatc gtcatgtatc ttaaatagtc ttatttaaga
    79201 tgttcagaac t
//
LOCUS       DAPHCV010000004        77885 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.004, whole genome shotgun sequence.
ACCESSION   DAPHCV010000004 DAPHCV010000000
VERSION     DAPHCV010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 77885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.004"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            7..483
                     /gene="agaB"
                     /locus_tag="RH033_000237"
     CDS             7..483
                     /gene="agaB"
                     /locus_tag="RH033_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HDV2989482.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMSITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNIGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            522..1325
                     /gene="agaC"
                     /locus_tag="RH033_000238"
     CDS             522..1325
                     /gene="agaC"
                     /locus_tag="RH033_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HDV2989483.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1315..2106
                     /gene="agaD"
                     /locus_tag="RH033_000239"
     CDS             1315..2106
                     /gene="agaD"
                     /locus_tag="RH033_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HDV2989484.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            2107..2874
                     /locus_tag="RH033_000240"
     CDS             2107..2874
                     /locus_tag="RH033_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HDV2989485.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSHLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHNYFTCMIDEMCRR"
     gene            complement(2924..3787)
                     /gene="rsmI"
                     /locus_tag="RH033_000241"
     CDS             complement(2924..3787)
                     /gene="rsmI"
                     /locus_tag="RH033_000241"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDV2989486.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQGE"
     gene            3853..5889
                     /gene="lpoA"
                     /locus_tag="RH033_000242"
     CDS             3853..5889
                     /gene="lpoA"
                     /locus_tag="RH033_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HDV2989487.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSELTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            5847..6242
                     /locus_tag="RH033_000243"
     CDS             5847..6242
                     /locus_tag="RH033_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDV2989488.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDVWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRITVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            6262..6852
                     /gene="diaA"
                     /locus_tag="RH033_000244"
     CDS             6262..6852
                     /gene="diaA"
                     /locus_tag="RH033_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HDV2989489.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            6862..7437
                     /gene="yraP"
                     /locus_tag="RH033_000245"
     CDS             6862..7437
                     /gene="yraP"
                     /locus_tag="RH033_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HDV2989490.1"
                     /translation="MKALSPIAVLLSALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(7550..8590)
                     /locus_tag="RH033_000246"
     CDS             complement(7550..8590)
                     /locus_tag="RH033_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDV2989491.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPLLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLV"
     gene            complement(8663..9298)
                     /locus_tag="RH033_000247"
     CDS             complement(8663..9298)
                     /locus_tag="RH033_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDV2989492.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            9426..9944
                     /gene="yhbO"
                     /locus_tag="RH033_000248"
     CDS             9426..9944
                     /gene="yhbO"
                     /locus_tag="RH033_000248"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HDV2989493.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVAIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(9924..10367)
                     /locus_tag="RH033_000249"
     CDS             complement(9924..10367)
                     /locus_tag="RH033_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HDV2989494.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRNSGTEQA"
     gene            10418..10720
                     /gene="yhbQ"
                     /locus_tag="RH033_000250"
     CDS             10418..10720
                     /gene="yhbQ"
                     /locus_tag="RH033_000250"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HDV2989495.1"
                     /translation="MTPWFLYLIRTADDKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPKIKSD"
     gene            complement(10707..11210)
                     /locus_tag="RH033_000251"
     CDS             complement(10707..11210)
                     /locus_tag="RH033_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV2989496.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(11204..11728)
                     /locus_tag="RH033_000252"
     CDS             complement(11204..11728)
                     /locus_tag="RH033_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HDV2989497.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TASETKQTSVGEPC"
     gene            11937..12932
                     /locus_tag="RH033_000253"
     CDS             11937..12932
                     /locus_tag="RH033_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDV2989498.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFIPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            12941..13819
                     /locus_tag="RH033_000254"
     CDS             12941..13819
                     /locus_tag="RH033_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDV2989499.1"
                     /translation="MKYSLGPVLWYWPKETLEEFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAILDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            13900..14907
                     /locus_tag="RH033_000255"
     CDS             13900..14907
                     /locus_tag="RH033_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDV2989500.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMLQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(15025..16269)
                     /gene="mtr"
                     /locus_tag="RH033_000256"
     CDS             complement(15025..16269)
                     /gene="mtr"
                     /locus_tag="RH033_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HDV2989501.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAMGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(16423..18312)
                     /gene="deaD"
                     /locus_tag="RH033_000257"
     CDS             complement(16423..18312)
                     /gene="deaD"
                     /locus_tag="RH033_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HDV2989502.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFSGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(18305..18385)
                     /gene="yrbN"
                     /locus_tag="RH033_000258"
     CDS             complement(18305..18385)
                     /gene="yrbN"
                     /locus_tag="RH033_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HDV2989503.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(18492..19376)
                     /gene="nlpI"
                     /locus_tag="RH033_000259"
     CDS             complement(18492..19376)
                     /gene="nlpI"
                     /locus_tag="RH033_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HDV2989504.1"
                     /translation="MKPFLRWCFVVTALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(19485..21620)
                     /gene="pnp"
                     /locus_tag="RH033_000260"
     CDS             complement(19485..21620)
                     /gene="pnp"
                     /locus_tag="RH033_000260"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDV2989505.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YNGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(21867..22136)
                     /gene="rpsO"
                     /locus_tag="RH033_000261"
     CDS             complement(21867..22136)
                     /gene="rpsO"
                     /locus_tag="RH033_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDV2989506.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(22285..23229)
                     /gene="truB"
                     /locus_tag="RH033_000262"
     CDS             complement(22285..23229)
                     /gene="truB"
                     /locus_tag="RH033_000262"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDV2989507.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(23229..23630)
                     /gene="rbfA"
                     /locus_tag="RH033_000263"
     CDS             complement(23229..23630)
                     /gene="rbfA"
                     /locus_tag="RH033_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDV2989508.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(23789..26461)
                     /gene="infB"
                     /locus_tag="RH033_000264"
     CDS             complement(23789..26461)
                     /gene="infB"
                     /locus_tag="RH033_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDV2989509.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(26486..27973)
                     /gene="nusA"
                     /locus_tag="RH033_000265"
     CDS             complement(26486..27973)
                     /gene="nusA"
                     /locus_tag="RH033_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDV2989510.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(28001..28453)
                     /gene="rimP"
                     /locus_tag="RH033_000266"
     CDS             complement(28001..28453)
                     /gene="rimP"
                     /locus_tag="RH033_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDV2989511.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(28660..28736)
                     /locus_tag="RH033_000267"
     tRNA            complement(28660..28736)
                     /locus_tag="RH033_000267"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28700..28702),aa:Met,seq:cat)
     gene            29084..30427
                     /gene="argG"
                     /locus_tag="RH033_000268"
     CDS             29084..30427
                     /gene="argG"
                     /locus_tag="RH033_000268"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDV2989512.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQVENLENKGQ"
     gene            complement(30435..32060)
                     /locus_tag="RH033_000269"
     CDS             complement(30435..32060)
                     /locus_tag="RH033_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDV2989513.1"
                     /translation="MTVFNKFVRSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFFAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALKQKTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQIFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPKTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(32517..32603)
                     /locus_tag="RH033_000270"
     tRNA            complement(32517..32603)
                     /locus_tag="RH033_000270"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32567..32569),aa:Leu,seq:gag)
     gene            complement(32618..32950)
                     /gene="secG"
                     /locus_tag="RH033_000271"
     CDS             complement(32618..32950)
                     /gene="secG"
                     /locus_tag="RH033_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDV2989514.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(33178..34515)
                     /gene="glmM"
                     /locus_tag="RH033_000272"
     CDS             complement(33178..34515)
                     /gene="glmM"
                     /locus_tag="RH033_000272"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDV2989515.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(34508..35356)
                     /gene="folP"
                     /locus_tag="RH033_000273"
     CDS             complement(34508..35356)
                     /gene="folP"
                     /locus_tag="RH033_000273"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDV2989516.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(35446..37380)
                     /gene="ftsH"
                     /locus_tag="RH033_000274"
     CDS             complement(35446..37380)
                     /gene="ftsH"
                     /locus_tag="RH033_000274"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDV2989517.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(37480..38109)
                     /gene="rlmE"
                     /locus_tag="RH033_000275"
     CDS             complement(37480..38109)
                     /gene="rlmE"
                     /locus_tag="RH033_000275"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HDV2989518.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            38235..38528
                     /gene="yhbY"
                     /locus_tag="RH033_000276"
     CDS             38235..38528
                     /gene="yhbY"
                     /locus_tag="RH033_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDV2989519.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(38684..39160)
                     /gene="greA"
                     /locus_tag="RH033_000277"
     CDS             complement(38684..39160)
                     /gene="greA"
                     /locus_tag="RH033_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDV2989520.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            39408..40841
                     /gene="dacB"
                     /locus_tag="RH033_000278"
     CDS             39408..40841
                     /gene="dacB"
                     /locus_tag="RH033_000278"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDV2989521.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLRRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(40882..42054)
                     /gene="cgtA"
                     /locus_tag="RH033_000279"
     CDS             complement(40882..42054)
                     /gene="cgtA"
                     /locus_tag="RH033_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HDV2989522.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(42070..43035)
                     /locus_tag="RH033_000280"
     CDS             complement(42070..43035)
                     /locus_tag="RH033_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDV2989523.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLANPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(43162..43419)
                     /gene="rpmA"
                     /locus_tag="RH033_000281"
     CDS             complement(43162..43419)
                     /gene="rpmA"
                     /locus_tag="RH033_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDV2989524.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(43440..43751)
                     /gene="rplU"
                     /locus_tag="RH033_000282"
     CDS             complement(43440..43751)
                     /gene="rplU"
                     /locus_tag="RH033_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDV2989525.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            44009..44980
                     /gene="ispB"
                     /locus_tag="RH033_000283"
     CDS             44009..44980
                     /gene="ispB"
                     /locus_tag="RH033_000283"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HDV2989526.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            45208..45486
                     /gene="sfsB"
                     /locus_tag="RH033_000284"
     CDS             45208..45486
                     /gene="sfsB"
                     /locus_tag="RH033_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HDV2989527.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWIIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(45534..46793)
                     /gene="murA"
                     /locus_tag="RH033_000285"
     CDS             complement(45534..46793)
                     /gene="murA"
                     /locus_tag="RH033_000285"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDV2989528.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(46848..47102)
                     /gene="ibaG"
                     /locus_tag="RH033_000286"
     CDS             complement(46848..47102)
                     /gene="ibaG"
                     /locus_tag="RH033_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HDV2989529.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(47262..47555)
                     /gene="mlaB"
                     /locus_tag="RH033_000287"
     CDS             complement(47262..47555)
                     /gene="mlaB"
                     /locus_tag="RH033_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HDV2989530.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(47555..48190)
                     /gene="mlaC"
                     /locus_tag="RH033_000288"
     CDS             complement(47555..48190)
                     /gene="mlaC"
                     /locus_tag="RH033_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HDV2989531.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(48209..48760)
                     /gene="mlaD"
                     /locus_tag="RH033_000289"
     CDS             complement(48209..48760)
                     /gene="mlaD"
                     /locus_tag="RH033_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HDV2989532.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(48765..49547)
                     /gene="mlaE"
                     /locus_tag="RH033_000290"
     CDS             complement(48765..49547)
                     /gene="mlaE"
                     /locus_tag="RH033_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HDV2989533.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(49555..50364)
                     /gene="mlaF"
                     /locus_tag="RH033_000291"
     CDS             complement(49555..50364)
                     /gene="mlaF"
                     /locus_tag="RH033_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HDV2989534.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAALLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            50574..51551
                     /locus_tag="RH033_000292"
     CDS             50574..51551
                     /locus_tag="RH033_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDV2989535.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            51565..52551
                     /gene="kdsD"
                     /locus_tag="RH033_000293"
     CDS             51565..52551
                     /gene="kdsD"
                     /locus_tag="RH033_000293"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HDV2989536.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPKSSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            52572..53138
                     /gene="kdsC"
                     /locus_tag="RH033_000294"
     CDS             52572..53138
                     /gene="kdsC"
                     /locus_tag="RH033_000294"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HDV2989537.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            53135..53710
                     /gene="lptC"
                     /locus_tag="RH033_000295"
     CDS             53135..53710
                     /gene="lptC"
                     /locus_tag="RH033_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HDV2989538.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            53679..54236
                     /gene="lptA"
                     /locus_tag="RH033_000296"
     CDS             53679..54236
                     /gene="lptA"
                     /locus_tag="RH033_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HDV2989539.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            54243..54968
                     /gene="lptB"
                     /locus_tag="RH033_000297"
     CDS             54243..54968
                     /gene="lptB"
                     /locus_tag="RH033_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HDV2989540.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            55016..56449
                     /gene="rpoN"
                     /locus_tag="RH033_000298"
     CDS             55016..56449
                     /gene="rpoN"
                     /locus_tag="RH033_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDV2989541.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            56472..56759
                     /gene="hpf"
                     /locus_tag="RH033_000299"
     CDS             56472..56759
                     /gene="hpf"
                     /locus_tag="RH033_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HDV2989542.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56877..57368
                     /gene="ptsN"
                     /locus_tag="RH033_000300"
     CDS             56877..57368
                     /gene="ptsN"
                     /locus_tag="RH033_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HDV2989543.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            57414..58268
                     /gene="rapZ"
                     /locus_tag="RH033_000301"
     CDS             57414..58268
                     /gene="rapZ"
                     /locus_tag="RH033_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDV2989544.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVFLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            58265..58537
                     /gene="npr"
                     /locus_tag="RH033_000302"
     CDS             58265..58537
                     /gene="npr"
                     /locus_tag="RH033_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HDV2989545.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            58750..59382
                     /gene="yrbL"
                     /locus_tag="RH033_000303"
     CDS             58750..59382
                     /gene="yrbL"
                     /locus_tag="RH033_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HDV2989546.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(59379..60107)
                     /gene="mtgA"
                     /locus_tag="RH033_000304"
     CDS             complement(59379..60107)
                     /gene="mtgA"
                     /locus_tag="RH033_000304"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HDV2989547.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MAERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEQA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGESFMQQHQLD"
     gene            complement(60104..60757)
                     /gene="elbB"
                     /locus_tag="RH033_000305"
     CDS             complement(60104..60757)
                     /gene="elbB"
                     /locus_tag="RH033_000305"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HDV2989548.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60987..63323)
                     /gene="arcB"
                     /locus_tag="RH033_000306"
     CDS             complement(60987..63323)
                     /gene="arcB"
                     /locus_tag="RH033_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HDV2989549.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(63419..64348)
                     /locus_tag="RH033_000307"
     CDS             complement(63419..64348)
                     /locus_tag="RH033_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDV2989550.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARERGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            65023..69483
                     /gene="gltB"
                     /locus_tag="RH033_000308"
     CDS             65023..69483
                     /gene="gltB"
                     /locus_tag="RH033_000308"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HDV2989551.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNNDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEDHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVEQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITITGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            69496..70914
                     /gene="gltD"
                     /locus_tag="RH033_000309"
     CDS             69496..70914
                     /gene="gltD"
                     /locus_tag="RH033_000309"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HDV2989552.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDLSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHSMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            71098..72225
                     /locus_tag="RH033_000310"
     CDS             71098..72225
                     /locus_tag="RH033_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDV2989553.1"
                     /translation="MESLSERTTAGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEF
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNPDARSFYE
                     KETLRCGWSVRQLERQITTQFYERTLLSHDKSAMLQQHAPAEMHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGAGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(72285..72749)
                     /locus_tag="RH033_000311"
     CDS             complement(72285..72749)
                     /locus_tag="RH033_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDV2989554.1"
                     /translation="MIMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGTAGTARQCEEFHHEDDYQ
                     LCSAIENEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(72746..73621)
                     /gene="nanK"
                     /locus_tag="RH033_000312"
     CDS             complement(72746..73621)
                     /gene="nanK"
                     /locus_tag="RH033_000312"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HDV2989555.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALHDA
                     LSALVSPLQVHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTDLPTIA
                     INDAQAAAWAEYQALEGDITEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHSSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(73618..74307)
                     /locus_tag="RH033_000313"
     CDS             complement(73618..74307)
                     /locus_tag="RH033_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDV2989556.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(74355..75845)
                     /gene="nanT"
                     /locus_tag="RH033_000314"
     CDS             complement(74355..75845)
                     /gene="nanT"
                     /locus_tag="RH033_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HDV2989557.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHGGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYDPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGRAKNDLVKTKS"
     gene            complement(75955..76848)
                     /gene="nanA"
                     /locus_tag="RH033_000315"
     CDS             complement(75955..76848)
                     /gene="nanA"
                     /locus_tag="RH033_000315"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDV2989558.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(76970..77761)
                     /gene="nanR"
                     /locus_tag="RH033_000316"
     CDS             complement(76970..77761)
                     /gene="nanR"
                     /locus_tag="RH033_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HDV2989559.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT        18983 a        21131 c        19444 g        18327 t
ORIGIN      
        1 cacacaatga gcagtccaaa tattctctta acccgtattg ataaccgtct ggttcacggt
       61 caggttggcg tgacctggac atccaccatc ggtgcgaatc tgctggtagt cgtggatgat
      121 gttgtcgcta acgatgatat tcaacagaaa ttaatgagta ttaccgcaga aacctacggc
      181 tttggcattc gtttctttac tatcgaaaaa accattaacg tcatcggcaa agctgcaccg
      241 catcaaaaga tcttcctgat ttgccgtacg ccacaaacgg tacgtaaatt ggtagaaggt
      301 ggtattgacc tgaaagatgt caacatcggc aatatgcatt tctcggaagg gaaaaagcaa
      361 atcagcagta aagtttatgt cgatgaccag gatctcacgg acttacgttt tattaaacaa
      421 cgtggcgtga atgttttcat tcaggacgtc cctggcgatc aaaaagaaca aatccctgac
      481 taaatctaaa atcgccttaa tattggtttg aggtaataaa aatgcatgaa ataaccctac
      541 tccagggatt atccctggcg gcgttagttt tcgttctggg gattgatttt tggctggaag
      601 ccttattttt attccgcccg ataatcgttt gtacccttac aggtgctatt ctcggtgata
      661 ttcagactgg cttaattacc ggtggcctga cagagttggc tttcgccgga ttaacccctg
      721 caggtggtgt tcagccgccc aacccgatta tggcgggtct gatgaccact gtcattgcat
      781 ggtctacggg cgttgatgcc aaaacggcaa ttggtcttgg cctgccgttt agtttgttaa
      841 tgcagtacgt cattctgttc ttctattccg ctttctcatt atttatgacc aaagccgata
      901 aatgcgcaaa agaggcggat acggcagcgt tttcccgact taactggaca acgatgctca
      961 tcgtcgcttc agcgtatgcg gtgattgctt tcctctgtac ttacctggca caaggggcga
     1021 tgcaggcgct ggtgaaagcg atgcccgcct ggctgaccca cggctttgaa gtggctggcg
     1081 gtattctgcc tgccgttggt tttggcttgc tgctgcgcgt gatgttcaaa gcgcaatata
     1141 tcccttatct gatcgccggt ttcctgttcg tttgctacat ccaggtcagc aacctgttgc
     1201 cggttgccgt actgggcgca ggctttgcgg tgtatgagtt tttcaatgcg aaatcccggc
     1261 agcaagcgca accgcagccc gttgccagta aaaatgaaga agaggactac agcaatggga
     1321 tctgaaatca gtaaaaaaga tatcacccgt ctgggctttc gttcgtcact gctacaagca
     1381 agctttaact acgaaaggat gcaggcaggc ggttttacct gggcgatgtt gccgatcctg
     1441 aaaaagattt ataaggacga caaaccgggc ctaagcgcgg cgatgaaaga taacctcgaa
     1501 tttattaata cccaccctaa tctggtcgga ttcctgatgg ggttattaat ttcgatggaa
     1561 gaaaaaggag aaaaccgcga caccattaaa ggcctcaaag tggcactgtt tggcccaatc
     1621 gccgggattg gcgatgcgat tttctggttc actttgttgc cgattatggc ggggatttgc
     1681 tcatcgtttg ccagtcaggg aaacctgctg gggccgattc tatttttcgc cgtttacctg
     1741 cttatctttt tcctgcgcgt cggctggacc cacgtcggtt attcagtcgg cgtgaaggcg
     1801 atcgataaag tgcgagagaa ctcgcagatg attgcccgtt cggcaaccat cctcgggatc
     1861 acagtaatcg gcgggctgat cgcttcgtat gtgcatatta acgtggtgac atcgtttgcc
     1921 atcgacagta cccacagcgt cgcgctgcag caggatttct tcgataaagt cttcccgaat
     1981 attttaccga tggcctacac cctgctgatg tattacttcc tgcgggtgaa aaaagcgcat
     2041 ccggtgctgt taatcggcgt gacgtttgtt ctttctattg tttgttctgc attcggcatt
     2101 ttgtaaatgg aacgaggcac cgcgtctggc ggtgcctctt tacttaagga atttcatcct
     2161 gtgcaaaccc ttcaacaagt tgaaaactat acggcgttaa gtgaacgtgc cagcgaatat
     2221 ttattggccg tgatccgtag caaaccggat gccgtgattt gcctggcgac cggagccacg
     2281 ccattactga cgtatcatta tctggtagaa aaaatccacc agcagcaggt tgatgtcagc
     2341 catctcacct tcgtgaagct cgacgaatgg gtggatctgc cattaacgat gcctggtacc
     2401 tgcgaaactt tcctgcaaca gcatatcgtg cagccgctgg ggctacgtga agaccagctc
     2461 atcagctttc gctccgaaga gataaatgag acagagtgcg aacgggtaac gaacctgatt
     2521 gcgcgcaaag gcggtctgga tttatgcgtt ctcggattgg ggaaaaacgg tcatcttggg
     2581 ctgaacgaac cgggagaaag cctgcaaccg gcctgccata tcagtcaact tgatgccaga
     2641 acacagcaac atgagatgtt aaaaaccgcg ggccgtcctg tgactcgtgg gatcacctta
     2701 ggcctgaagg atattctcaa tgcccgcgaa gttttgttac tggtgactgg cgaaggaaag
     2761 caggatgcga cagaacgttt tctcacggct aaagtctcta ccgctatccc ggcttcattt
     2821 ttatggctgc ataactattt cacatgcatg attgatgaga tgtgtcgtcg ttaactcgcc
     2881 tgctacaggc gttgcaacgc tattcctgct gcgacgcctg taatcactct ccctgctgct
     2941 ccagcgcata cttatacagc gcatttttct tcacgccgtg aatttctgct gccagcgccg
     3001 ccgctttttt cagcggcagt tctgcctgca acagcgccag cgtgcgcaga gcatcggcgg
     3061 gtaagtcttc ttcctgtgct ttatggcctt cgacaatcag taccatttcg cctttgcgac
     3121 ggttttcatc ttctttgacc cacgccagca gctcgccaac gggcgcgccg tgaatggttt
     3181 cccaggtttt ggtcagctca cgcgccagaa cgacgtagcg ggattcgcct aataccgcaa
     3241 cgatatcttc caggctatct aacagacggt gggtagattc ataaaaaatc agcgtgcgcg
     3301 gctccgcttc aatggctttt agcgcatcac ggcggccttt tgatttggca ggtaaaaagc
     3361 cttcataaca aaaacgatcg gatggtaaac ccgcggcgct taacgcagtg atagcagcac
     3421 acggcccggg tagcggcacc acgcgaatcc ccgcttcacg gcaggtacgc accagatggt
     3481 agccaggatc gttaattagc ggcgttccgg catcggaaac cagcgcaatg ttttgcccct
     3541 cttgcagctt cgccagcagc gtttcggctt tttgttgttc gttatggtcg tgcagcgcaa
     3601 acaaccgagc attaatccca aaatgttgca gcaataaacc ggtgtgacgc gtatcctcgg
     3661 cggcaatcag atcaacggcc tgtaatacct ccaacgcacg ctgggtgata tccgccagat
     3721 tgccgattgg cgtcggtaca atgtaaagct ggccctgaga attatccgcc gattggtgtt
     3781 gtttcattgt gtcgtccgta ttgccgattt aatattgagc attgcgtaaa aaaaaatatc
     3841 actggataca ttatggtacc ctcaacattt tctcgtttga aagccgcgcg ttgtctgcct
     3901 gttgttctgg cagccctgat tttcgccggt tgtggcaccc atactcccga tcagtccact
     3961 gcttatatgc agggcacggc gcaggctgat tctgcctttt acctgcaaca gatgcagcaa
     4021 agttctgatg ataccaggat caactggcaa ttactcgcca ttcgtgcact ggtgaaagaa
     4081 ggtaaaaccg gacaggcggt ggagttgttt aaccaactac cgcaagaact gaacgattct
     4141 cagcgtcgcg agaaaacact gctggcggca gagattaaac tggcgcagaa agattttgct
     4201 ggcgcgcaaa acttgctggc gaaaatcaca cctgccgatt tagaacaaaa ccagcaagcc
     4261 cgttactggc aggcaaaaat cgatgccagc caggggcgtc cttccattga tttactgcgc
     4321 gcgttaattg ctcaggaacc gctgctcggt gcgaaagaaa agcagcagaa tattgacgcc
     4381 acatggcagg cgctctcctc catgactcag gagcaggcga atacgctggt gatcaacgcc
     4441 gacgaaaata ttctgcaagg ctggctggat ctgcagcgcg tctggtttga taaccgtaac
     4501 gatcctgaca tgatgaaagc tgggatcgcc gactggcaga aacgttatcc gaacaacccg
     4561 ggcgcgaaaa tgctgccaac gcagttggtt aacgtaaaag cgtttaaacc cgcatcgacc
     4621 aacaaaatcg ccctgctgtt gccgctgaat ggccaggcag cggtatttgg tcgcactatt
     4681 cagcaaggct ttgaagcggc gaaaaatatc ggcactcagc cagtggcggc ccaggtagca
     4741 gccgcacctg ccgcagacgt agcagaacaa cctcagccgc aaaccgtgga tggcgttgcc
     4801 agcccggcac aagcttcggt tagcgagctg accggtgaac agcctgcggc ccagccggta
     4861 cctgtaagcg ccccggcgac aagcaccgca gcggtaagcg cacccgcaaa tccatccgca
     4921 gagctgaaaa tctacgatac ctcatcacaa ccacttagcc agatcttaag ccaggttcag
     4981 caggatggtg cgagtattgt ggtcggtccg ttgctgaaaa ataacgttga agagttgctg
     5041 aagagcaaca ccccgctgaa cgtgctggca ctgaaccagc cggagaacat cgaaaaccgc
     5101 gtcaatattt gttacttcgc gctttcaccg gaagacgaag cgcgcgatgc agcgcgtcat
     5161 attcgtgacc agggtaaaca agcgccgctg gtgctgatcc cacgcagttc attgggcgac
     5221 cgcgtagcca atgcgtttgc gcaagagtgg cagaaactgg gcggcggcac cgttctgcaa
     5281 caaaaatttg gttccaccag cgaattacgc gcgggtgtta acggcggttc tggtattgct
     5341 ttaacgggta gcccgattac tcccagagcg acaaccgact ccggcatgac gaccaacaat
     5401 ccaacgctgc aaaccacgcc aaccgatgac cagttcacca ataatggcgg tcgtgtcgat
     5461 gcggtgtaca ttgtggcaac gccgggtgaa atcgctttta tcaaaccgat gatcgccatg
     5521 cgtaacggta gccagagcgg tgcaacgctg tacgccagtt cccgcagcgc acaaggcacc
     5581 gcaggcccgg atttccgtct ggagatggaa ggcttgcagt atagcgaaat cccgatgctg
     5641 gcaggcggta atctgccgtt aatgcagcag gcgctcagcg cggtgaacaa cgattattcg
     5701 ctggctcgca tgtatgcgat gggcgtcgat gcctggtcgt tggcaaatca tttctcacaa
     5761 atgcgccagg ttcagggttt tgaaatcaac ggtaataccg gaagtctgac ggctaacccg
     5821 gattgcgtga ttaacaggaa gttatcatgg ctacagtacc aacaaggtca ggtagtcccc
     5881 gccagttaac caccaaacag accggcgatg tgtgggaagc acaagcgcgt cgctggctgg
     5941 aaggcaaagg actgcggttt atcgccgcta acgtgaacga gcgtggcggc gagatcgatc
     6001 tgataatgcg tgaaggccgg atcaccgttt ttgtcgaggt gcgttaccgc cgctctgcgc
     6061 tttatggcgg cgcggcagcc agtgtgaccc gcagcaaaca acacaaatta ttacagactg
     6121 cccgcttgtg gctcgcgcgt cataatggga gttttgatac tgtggattgc cggttcgatg
     6181 tggtagcctt caccgggaat gaggttgagt ggattaagga tgcctttaat gaccactcat
     6241 aattaaggtt taaggattag cgtgcaagaa agaattaaag cttgcttcac tgaaagcatt
     6301 caaactcaaa ttgcggcggc agaggcgctt ccggatgcca tctcgcgtgc agctatgacg
     6361 ctggttcagt ctctgctcaa tggcaacaaa atcctctgtt gtggtaatgg aacttccgct
     6421 gccaatgcac agcattttgc tgccagcatg atcaaccgtt tcgaaacgga gcggcccagc
     6481 ttacctgcca ttgcactaaa tactgataat gttgtcttaa cggcgattgc caacgatcgc
     6541 ttacatgatg aagtgtatgc aaaacaggtg cgggcgctgg gtcatgcggg agatgtattg
     6601 ttagccattt ccacccgtgg caacagccgc gatattgtaa aagcggtaga agctgccgtt
     6661 acgcgtgata tgaccattgt ggcattaacc ggctatgacg gcggtgaact tgcaggtttg
     6721 ttagggccac aggatgtgga gatccgcatt ccttcgcatc gtagtgctcg cattcaggaa
     6781 atgcatatgc tgacggtaaa ttgcctgtgc gatctgatcg ataacacgct tttccctcac
     6841 caggatgttt aaggagaata catgaaggca ttatcgccaa tcgcagtcct tctttccgcg
     6901 ctgctgttgc aaggttgtgt tgccgctgcc gtagtgggta ccgctgctgt aggtaccaaa
     6961 gccgcaactg acccacgcag tgtcggcacc caggtggacg atggtaccct ggaagtgcgc
     7021 gtgaacagcg cattgtcgaa agacgaacaa attaagaaag aagcgcgcat taatgtcacg
     7081 gcctatcagg gcaaagtgct gctggttggg cagtcaccaa acgctgaact ttcggctcgc
     7141 gccaaacaga ttgctatggg cgtagacggt gccaacgaag tgtataacga gattcgtcag
     7201 ggccagccga ttggtctggg cgaagcgtct aacgatacgt ggatcaccac caaagtgcgt
     7261 tcgcagctct taaccagcga cctggtgaaa tcgtccaacg tgaaagtgac caccgaaaac
     7321 ggtgaagtgt tcctgatggg gctagtgact gaacgtgaag cgaaagcggc ggctgatatt
     7381 gccagccggg taagcggcgt gaagcgggta accacggcgt ttacgtttat taaatagcag
     7441 cccttgtaat gcctgatgcg acgcttgcag cgtcttatca ggcctacaac gatgcaaacc
     7501 gtaggtcgga taaggcgttt acgccgcatc cgacatgttt tccctcaaat caaaccaaca
     7561 gcgccagccc gccgacaatc acaccagaca ctgccaccat cgcccccgtc agccataagg
     7621 ctttcgccgg gaacgctttg cgcagcataa tcagtgacgg caaactcacc gccgggagcg
     7681 tcatcaacaa tgccagcgcc ggagcagttc ccatacctgc cagcatcatc gtttgtacaa
     7741 tcggaatttc tgctgccgtg ggaatgacaa acaagcatcc tgctaccgcc atcgccacca
     7801 cccacatcag gctgttatcg acagcaccat cagcatgggg gaataaccac acgcgagcgg
     7861 cacccaacac cagtactgca aggatgtaaa ccgggatcgt actccagaaa agcgtccata
     7921 gcgccctgcc ccagcggcta aaaaatcctc cctgtgcttc cggtatgtca atttcgaccg
     7981 gtgcctgcgt ttgcggtgtt tcacgcaccc atttttgcac cagcgtcgca atcagcaaca
     8041 ccatcaccag ccccgccacc agacgaatcg ccgcaaaacc ccagccgagg acgaacccca
     8101 taaacaccag cgtcgccggg tttaacaacg gattccccat ccagaatgcc agcgcaccgc
     8161 ccatcgacac ctgttggcga cgcattcccg ccgcgaccgg agccgcacag caggtacaca
     8221 tcatgcccgg cagcgaaaac agcgttccca gcagcgtgcc gcgaaagcgc gattgcccaa
     8281 gcgtacgaag caaccagtca cgcgggatca gcacctgaat caacgaaccg aggatcaccc
     8341 ccagcaccgc cgctttccat accgcgagga aatagatcat cgcgtaatcc aacgccgcct
     8401 gccacggatt agcatccgcc tgcgcaagga tagatttacc gatactgtgg gtttcggcag
     8461 cagtaaacgc tttgccgtag taaggttgcc atttcacata ccagagacca gcaatgacaa
     8521 cgagaaagaa aagcgcgggt ttccaccact gaatgggcgt tgccgcctga gatgaagact
     8581 gaccagtcat agcattcccc aggaataagt tatgataatt gagcgcgtga atattacgct
     8641 cactatcaat tcttgggaat aattattcag ctcttttgcg taattctgaa gagcttaaaa
     8701 tcgtcacacc ttcatgctca ggccgcatcg cctctgccag catgacccgc gccacgtctc
     8761 tcgcatcaat ggatttccag ttacccggta acaaacggaa cagcggcgca aaaagcgttt
     8821 cgttcatccg ctgtttgcta cgatcgccca gtaacatcga cgggcgagca atggtcaatt
     8881 tcggccagtt ctgggcgatt aatgcttctt ccatctcccc tttgacgcgg ttatagaaaa
     8941 acggcgagtg ggcattagca cccatagcac tgaccaccag catatgctgc gcaccgagcc
     9001 gccgcccggt taatgcggta tccaccacca gcgtgtaatc ggcatgaata aacgcctctt
     9061 tactccccgc ttctcgccgc gtggtgccga gacaacaaaa cacaatgtcg atgggatcgg
     9121 tgacctgcgc cagcgcgtcg gtcagttgcg gatcatgggg attaaacaca ccgggcatat
     9181 cgcccaacgg acgtcgcgtc ggcgcggcta tggcgttaac tttcggttca ttaatcaaca
     9241 tccgcagcag gtgaccaccc accagccccg ttgcgcctgt aatcagtacc tggctcatct
     9301 tcgctccttt acagaattgt ctgccttgcg ctccccggct catgcaccag gcttaatagt
     9361 ctacgaggta attatccccg attgtgggaa attcgcctca tccaatgcaa caacgcggag
     9421 gaagcatgag taagaaaatt gccgttttaa tcactgatga atttgaggat tcagaattta
     9481 cttcacccgc agacgagttc cgtaaagccg gacacgaagt gattaccatt gaaaaacaag
     9541 cgggtaaaac ggtgaaaggc aaaaaaggag aagccagcgt ggccatcgat aaatccatcg
     9601 atgaagtgac gccagcggag tttgatgccc tgctgctacc gggcggccat tcaccggatt
     9661 atctgcgtgg cgacaaccgt tttgtcactt tcacccgtga ttttgtgaat agcggcaaac
     9721 ctgttttcgc catctgtcac ggtccgcagc tgctgatcag cgccgatgtg attcgggggc
     9781 gcaaactgac tgccgttaaa ccgatcatta ttgatgttaa aaacgcgggc gcggaatttt
     9841 acgatcagga agtggtggtc gataaagatc agctggttac cagccgtaca ccggacgatc
     9901 tgccagcgtt taaccgcgag gcattacgcc tgctcggtgc ctgagttacg tagccagatc
     9961 atttttttac caaagcccag cgtgttgtcg gtgaatttga tttcatcgag ccggatttcc
    10021 cacaccgggg ccgacagcat tctggcaacc ggaaagcggc gattgtacgc ctggcgcgca
    10081 aggtcgcttt cctcaccttc aagcctgcgg atctcgcctt taaattgcac gccgcgaatt
    10141 aacgctaccg ttttcggctg accgtttacc gttccggcaa ctgccgcctg cggcccactc
    10201 atctgcgcgt ggcgcgtttt ttcttccgtc aaaatgtaga aggcgacttt ctgcgcatca
    10261 aaaagataaa aggcattagc gcaccagagt tccccttcct gctgcacaca ccaggtgaca
    10321 acatgttgtt ttgccagcca acggctgatg gcggtgagtg tttccattgc tgttctcttt
    10381 tatactgtgg gcctgacctt aacatgcgac gcagacgatg acaccctggt ttctttactt
    10441 gatccgtacc gccgacgata agctttatac cgggatcacc acagatgtcg aacgccgcta
    10501 tcagcagcac caaagcggca aaggggcgaa agcactgcgc gggaaaggag aactaacgct
    10561 ggcgttttcc gcgccagtgg gcgatcgttc gctggcacta cgggcggaat atcgcgttaa
    10621 gcaactgacc aaacggcaaa aagagcgtct ggtagcggaa ggcgcagggt ttgcagaact
    10681 gttgagcagc ctgcaaaccc caaagattaa aagcgattga agtgctcgtg atactcaacc
    10741 agaccagtta caccgttcag cgcgtcatcc gctaaacgat gtacctggaa ggcactttca
    10801 gtgcccggcc agcggcaacg cagatcgtga tgtgccgcca gttcaaagcc gaaacggctg
    10861 tacagcgccg gatcgcccag cgtcaccact gcggcatagc cgaactcatt aagcgaatcg
    10921 agtccttcat agaccagttg gcgtgccagc ccctgtccac ggtatttttc atctaccgcc
    10981 agcggtgcca tgccgaccca ttgcaggtct tcgccctgca catcaaccgg actaaatgcc
    11041 acatagccaa tgacctgacc ttcgtcatct gtcgccacca gccccagcgt cagaaagcca
    11101 tcttcacgca gatcgtgaac cagcttcgct tccgcatcac tttcgaatga acgacgcagc
    11161 agggcatcaa tacccggcgc atcaatggga atttctactc gaattagcat ggttcaccta
    11221 ccgatgtctg tttggtttca gacgcggttt tcattcccgc ctcaacaaaa tccgccagtt
    11281 gcagcagcat catgcgtaag gctttcggca tttgctccag ttcaatggcg tccatcaggt
    11341 ttttcacata cagccccagc tccgtatcgc cttcaattac cagccgacgc tggaagaaga
    11401 gcgtatccgg atcttgttta cgcgccgcaa tcatcagcag atcgctggcg tcggcactaa
    11461 aactcacatc agcttgcgcg ttctggctaa cgaccagttt gccattcacc accgaggtaa
    11521 accattgcag gtcaatatca cgcacatgaa tacttaacca gcggccttca agaaactcca
    11581 gctcgccatc atccaacgcc tggcggaatt gccagcttaa gacctgctca agaacctggc
    11641 gttttagcgc aaatggcgtc agttttaccg gtacactcaa cagagatggc cccaaatgca
    11701 caatacggga acgcagttta tccaacacga gttttactcc ctgtttcaac aatcatccta
    11761 ttttgccata tcagaaaaat aacatagcgg tataaatcaa caattccata tgaaattgct
    11821 gctaccacca atacaacttt aactgcctta aatcaaaaat tgtcgcagca aggttaacta
    11881 aaatcccagt tcgttaacat ttttgcgctt tgatagcgca accttcagga aaaattatgg
    11941 agctgctctg ccctgccgga aatctcccgg cgcttaaggc ggccatcgaa aacggcgcag
    12001 atgctgttta tatcgggcta aaagatgata ccaatgcccg tcacttcgcc ggccttaact
    12061 ttaccgagaa aaaattgcag gaagcggtga gttttgtcca tcaacatcgc cgcaaacttc
    12121 acatcgcgat taacactttt gcgcatccgg acggttacgc ccgctggcag cgcgccgtgg
    12181 atatggcggc gcagttgggt gccgacgcgc tgatcctcgc cgacctcgcc atgctggagt
    12241 acgccgccga gcgttacccg catattgagc gtcatgtgtc agtgcaggct tcggcgacca
    12301 atgaagaggc gattaacttt tatcatcgcc attttgacgt tgcccgcgtg gtgctgccgc
    12361 gcgtgttgtc gattcatcag gtgaaacaac tggcacgggt cacacctgtc ccgctggaag
    12421 tgtttgcttt cggcagcctg tgcattatgt cggaaggccg ttgctatctg tcgtcgtatc
    12481 tgacgggtga gtcgcccaac accgtgggcg cgtgttctcc ggcccgtttc gtgcgctggc
    12541 agcaaacgcc gcagggactg gaatctcgcc tgaacgaagt gctgatcgac cgttatcagg
    12601 atggcgaaaa cgcaggttat ccgacgctgt gtaaagggcg ttatctggtg gacggcgagc
    12661 gctatcacgc gctggaagaa cccaccagtc tcaataccct ggaactgctg ccggagttaa
    12721 tggcagcgaa tattgcttcg gtgaaaattg aaggccgcca gcgtagcccg gcgtatgtca
    12781 gccaggtggc gaaagtctgg cgtcaggcta tcgaccgttg taaggccgat ccgcaaaact
    12841 tcataccgca aagcgcgtgg atggagacgc tcgggtcgat gtccgaaggc acgcagacca
    12901 cccttggcgc gtatcaccgt aaatggcagt gagaaaagca atgaaatatt ccttagggcc
    12961 agtgctgtgg tactggccaa aagagacgct ggaagaattt tatcagcagg ccgccgccag
    13021 cagcgccgac gtgatttatc ttggtgaagc ggtatgcagc aagcgccggg caaccaaagt
    13081 tggcgactgg ctggagatgg caaaatcact cgccggaagt ggtaagcaga ttgtgctctc
    13141 cacgctggcg ctggtgcagg catcctcaga actgggcgaa ctgaaacggt atgttgagaa
    13201 cggtgagttt ctgattgaag ccagcgatct cggcgtggtg aatatgtgcg ctgaacgcaa
    13261 actgccgttc gtcgccgggc acgcgctgaa ctgctacaac gcggtgacac tgaaaatatt
    13321 gctcaaacag ggcatgatgc gctggtgtat gccggtggag ctttcccgcg actggctggt
    13381 gaatctgctt aatcagtgcg atgagctggg cattcgcaac cagtttgaag tggaagtcct
    13441 gagctacggt catctgccgc tggcctactc cgcccgctgc tttaccgcgc gttcggaaga
    13501 ccgcccgaaa gatgagtgtg aaacctgctg cattaagtat ccgaacgggc gcaacgtgct
    13561 atcgcaggaa aaccaacaag tgtttgtact caatggcatt cagaccatga gcggctacgt
    13621 ttacaacctc ggtaacgagc tggcatccat gcaggggctg gtcgatgtgg tccgcctgtc
    13681 accgcagggt actgacactt tcgcgatact cgacgccttc cgcgccaatg aaaatggcgc
    13741 tgcaccactg ccgttgacgg caaacagcga ttgtaacggc tactggcggc ggctggccgg
    13801 gctggagctg caagcctaag taaatagctc actttgttaa caactttaac tactctttaa
    13861 tgcagtatta aagattaatc agtaacaaag tgagctgtta tgactgataa aaccattgcg
    13921 ttttcgctac tcgatctggc ccccattccc gaaggctctt cagcgcggga agcgttctcc
    13981 cactctctcg acctcgcccg tctggctgaa aagcgcggct atcatcgcta ctggctggcg
    14041 gaacaccaca atatgaccgg cattgccagt gctgcaacat cagttttgat tggctatctg
    14101 gcggcgaata ccactacgct gcatctgggg tctggcggcg tgatgttgcc taaccactca
    14161 ccattggtga tcgccgaaca gttcggcacg cttaatacgc tctatccggg gcgaatcgat
    14221 ttggggctgg gtcgtgcgcc gggtagtgac cagcgaacca tgatggcact acgtcgtcat
    14281 atgagcggtg atattgataa tttcccccgc gatgtcgcgg agctggtgga ctggtttgac
    14341 gcccgcgatc ccaatccgca tgtgcgcccg gtaccaggct atggcgagaa aatccccgtg
    14401 tggttgttag gctccagcct ttacagcgcg caactggcgg cgcagcttgg tctgccgttt
    14461 gcgtttgcct cacacttcgc gccggatatg ttgttccagg cgctgcatct ttatcgcagc
    14521 aacttcaaac cgtcggcacg actggaaaaa ccatacgcga tggtgtgcat caatattatc
    14581 gctgccgaca gcaaccgcga cgctgaattt ctgtttacct caatgctgca agcctttgtg
    14641 aaactacgcc gtggcgaaac cggacaacta ccgccaccga ttcaaaatat ggatcagttc
    14701 tggtcaccgt ctgagcagta tggcgtgcag caggcgctga gtatgtcgtt ggtaggtgat
    14761 aaagcgaaag tgcgtcatgg cttgcagtcg atcctgcgcg aaaccgacgc cgatgagatt
    14821 atggttaacg ggcagatttt cgaccaccag gcacgcctgc attcgtttga gctggcgatg
    14881 gatgtgaagg aagagttgtt ggggtagtgt gttttaacgt aggaagcctt atccgagatg
    14941 gcaacgctgt cctacgtagg cctgataagc gaagcgcatc aggcattgtg taggcagcag
    15001 aaatgtcgga taaggcaccg ctgattactg atacaccggc agcaaattaa agctcgataa
    15061 aatatgcacc agtgcgttgc caacgccaaa caccagaatc agcgcaatca tcggcttgcc
    15121 gccccagacg cggaatttcg ggctgccaaa gcgtttacgc gatgcgcggg ccaacagcgc
    15181 cggtacaatt gccgcccaga tagtagctgc taagccagca taaccaatgg catacaggaa
    15241 tccgttcgga aatagcagac cacccacaac aggcggggca aaggtcagca acgctgtttt
    15301 caaacggccc atagccgagt cgtcgaaacc aaacagatct gccagatagt caaacaaacc
    15361 cagcgttacg ccgaggaacg aacttgctac cgcaaagttt gagaacacga ccagcagaag
    15421 gtccaggcta cggctgttca gcacgccgct taatgcctgt accagcacat caatattacc
    15481 gcccttctcg gcgatgccga taaactccgg acgagggatg ttgcccatcg tcgccagcaa
    15541 ccagatggta tacagcgcca gtgccatcag cgtaccgtac accaggcatt tcacgatggt
    15601 tttcggatct ttgccgtaat acttcatcag gctcggcacg ttaccgtgat aaccaaacga
    15661 tgccagacag aatggcagcg tcatcagcag atacggtgca taagacgcat tgctttcggc
    15721 gacgttgaac aatgtcgcag gctgcacatg tcccagcagg ctaccgaagg tgaggaaaaa
    15781 cgtaatgact ttcgccccca gcacaatcgc cgtcatccgg ctgaccgctt tggtgctcaa
    15841 ccacaccaca aacgccacca gtagtgcaaa accaaatcca gctgcccgtg ccgggacgtt
    15901 caatgacatc tctgcgaagg tatgatgcag aatcgaaccg ctggcagaaa tataggcata
    15961 ggtcaggata tagagcacaa aggcaatgga aatgccgttg accacgttcc agcctttgcc
    16021 cagcaaatct ttggtgatgg tgtcaaagct cgaaccgatt cggtaattca ggttagcttc
    16081 cagaatcatc aagccggaat gcagcataca gaaccaggta aagatcagcg ccgccattga
    16141 ccagaagaac cacgccccgg acatgaccac aggcagagaa aacatccctg cgccaataat
    16201 ggtgccgccg ataatcacca cgccgccaag cagcgacggt gacgtttggg tggtggttag
    16261 tgttgccatg agggcttctc tccagtgaaa aatagtgcga ctgcgttgtt atgcattgca
    16321 ctgtaccagt acacgagtac aaaagacaga aaaaaagccc cgatggtaaa aatcggggct
    16381 gtatatatta ttttacagat tgtgttcgct gttcagcgat gattacgcat caccaccgaa
    16441 acgacgacga ccggtagagt catcacgacg cggagcgcgg ccttcacgac gttcgccgct
    16501 aaaacgacga ccatcaccac gaccaccttc acggcgttca ccgctgaagt tacgaccacc
    16561 ttcacgacgt tcgccactga aaccacgacc accgccacga cgctcaccgc cagtatgcgg
    16621 ctgtgcatcg cccagtaact gcatgttcat cggcttgttg agaatgcgag tgcgtgtaaa
    16681 gtgttgcagc acttcacccg gcataccttt cggcagttcg atggtggagt gagaagcaaa
    16741 cagcttgatg ttaccaatgt aacggctgct gatgtcgcct tcgttagcaa tcgcaccaac
    16801 gatatgacga acttcaacac catcatcgcg gcccacttca atgcggtaca gctgcatatc
    16861 gccaacatca cgacgttcac gacgcggacg atcttcacgg tcaccacgcg ggccacggtc
    16921 gttacgatcg cgcggaccac ggtcatcacg gtcacggaat tcacgtttcg gacgcatcgg
    16981 cgcatctggc ggaacaatca gagtacgttc accctgtgcc attttcagca gtgcggcggc
    17041 cagagtttcg agatccagct cttcaccttc tgccgtcggc tgaattttgc tcagcagtgc
    17101 acggtattga tccagatcgc tgctttccag ctgctgctgt actttagcgg cgaatttttc
    17161 cagacgacgt ttgcccagca gttctgcgtt cggcagttca acttccggaa tggtcagctt
    17221 catggtgcgt tcaatgttac gcagcagacg acgctcacgg ttctcaacga acagcagcgc
    17281 acggccagca cggcccgcac gaccagtacg accgatacgg tgaacgtaag actcagaatc
    17341 catcgggata tcgtagttaa ctaccaggct gatacgctca acgtccaggc cacgggccgc
    17401 aacgtcggtc gcaatcagga tgtccagacg accatctttc agtcgttcca gtgtctgctc
    17461 acgcagcgcc tggttcatgt caccgttcag cgcggcgctg ttgtagccgt tacgctcaag
    17521 ggcttctgcc acttccagag tcgcgttttt ggtacgaacg aagataatcg ccgcatcaaa
    17581 atcttccgct tccaggaaac gtaccagtgc ttcgttttta cgcatacccc agacagtcca
    17641 gtagctctgg ctgatgtcag gacgggtagt cacgctggac tgaatgcgca cttcctgcgg
    17701 ctctttcata aagcggcggg taatgcgacg aatcgcttcc ggcatggttg cagagaacag
    17761 agcggtctga tgaccttccg ggatctgcgc cataatagtt tcaacgtctt cgatgaagcc
    17821 catgcgcagc atttcgtcag cttcatccag aaccagaccg ctcagtttag aaaggtccag
    17881 agtgccacgt ttcaggtgat ccagcagacg gcccggagta ccgacaacga tctgcggccc
    17941 ctgacgcagg gcgcgtaatt gcacgtcata acgctggccg ccgtacaggg caaccacgtt
    18001 tacgccgcgc atgtgtttag agaaatccgt cattgcctca gcaacctgta ccgccagttc
    18061 gcgggtcggt gccaacacca gaatctgtgg tgctttcagc tcaggatcaa gattctgcag
    18121 cagaggtaaa gagaatgctg cagtttttcc gctccccgtc tgggccatac ccagaacgtc
    18181 gcggccattc agcagatgtg gaatacactc tgcctgaatt ggagatggtt tttcgtaacc
    18241 cagatcgtta agggcttcaa ggataggagc cttcaggccc agatctgcaa aagtggtttc
    18301 gaattcagcc atgtagtacg tgtgcctcaa aattaatggc ggccagtcta cataactcat
    18361 catgaaattg atcagcaatt ttcattgaaa agtgtgaacc ggctcaaagt aggtgtatta
    18421 acgaacaaca acgccctcac ccgttaaggt gatggcaatc aaaaaagatt acgggctgat
    18481 gtgtacgtca gctattgctg gtccgattct gccaggtcat cttggtcctg gcccaggagc
    18541 gataattcca acaatgcgta tcggtgctca acaaagttat gaacgttgtt ggcaaccgcc
    18601 agtttgaaca gtgccgtggc gctgtccaaa tcccccagac ttaggtagta cttacctaaa
    18661 tagaagttgg tttcactgag atgctcagcg agcgaggtgt tatccgttgc gtccgccttg
    18721 agcctttcca ttaacgtttg ttcgctaatg ttgcccaggt agaactcgac aatgttccat
    18781 ccccactgtt ccttatccga tttttcgaag tgctgtttca acacttcttt agcctgcttc
    18841 tcatcgagct tctgctcggc gagataaagc cacagactac ggaaaggatc attgggatcg
    18901 tcttgataaa acgccagcag atcatcttgc gctaacttgt cacgaccgcc gtaatataat
    18961 gcgatcccgc gattcaagtg cgcgtagttg taagttggat caagctcaag tacagaatca
    19021 aacgcttcat aggcagcatc aaaattgcct gcctgcgtta aatatatgcc taagtaattg
    19081 aatacttcag gcatatccgg tcggattgcc agcgcttgcg aaaaatcgtt acgcgctaat
    19141 gccctcagac cgagactatc atacaacact ccgcgctcat ataaaagctg tgcgcgttcg
    19201 tcatcggtta aagcccgact ggcaaggatt tgttccatac gtgccagaat cacttcctgc
    19261 tgtaaagtcg gttgcaatgg taccgcgagg acttcacttt tacgccagga agtattactg
    19321 catcctgcaa gcgtaagtgc tgtcacaacg aaacaccagc gcaaaaaagg cttcatttcc
    19381 cactcccgaa gaccacggtt gaatgaacgt cctgttcccg gttgctaaca aggcgtcctg
    19441 cccggttaaa agccccccgc agcagcggag ggcaaatggc aaccttactc gccctgttca
    19501 gcagccggag cttccggtgc tgcagcaggt tgagactgct cagtcgcttc tttaatgctc
    19561 agacggatac ggccctggcg atcaacttcc agaactttca ccggtacttc ctgacccatc
    19621 tgcaggtaat cggtcacttt ctcaacgcgt ttgtcagcga tctgagagat gtgtaccaga
    19681 ccttctttac cgccgccgat ggcaacaaat gcgccaaagt caacgatacg ggtcacttta
    19741 ccattgtaga cgcggcccac ttcgatttct gcagtgatct cttcgatacg acgaatagca
    19801 tgtttcgctt tctcgccgtc ggtcgctgcg attttcacag taccgtcatc ttcgatttcg
    19861 atggtggtgc cagtttcttc ggtcagagca cggataacag aaccgccttt accgataacg
    19921 tctttgatct tgtccgggtt gatcttgatg gtatggatac gcggagcgaa ctcagagata
    19981 tcgccgcgcg gcgcgttgat cgcctgttcc attacgccca ggatatgcag acgcgcacct
    20041 ttagcctggt tcagtgcaac ctgcatgatc tctttggtga taccttcaat tttgatatcc
    20101 atctgcaatg cagagatacc gtcgcgggaa cccgcaactt tgaagtccat atcgcccagg
    20161 tgatcttcgt cgcccaaaat gtcagacagt acaacgtagt tgtcgccttc tttcaccaga
    20221 cccattgcga tacccgcaac ggcagcttta attggcacac ctgcgtccat cagcgccaga
    20281 gacgcgccgc acacggaagc catagaagaa gaaccgttgg attcagtgat ttcagacaca
    20341 acacgtacgg tatacgggaa tttgtccata tccggcatga ctgccagcac gccgcgcttc
    20401 gccagacgac cgtgaccaat ttcacgacgc ttcggagaac cgaccatgcc ggtttcgcct
    20461 acagagtacg gagggaagtt gtagtggaac aggaaggtgt cagtacgttc gcccatcagt
    20521 tcatcaagaa cctgcgcgtc acgtgcagta cctagcgttg cggtaaccag cgcctgagtt
    20581 tcaccacggg tgaacagcgc agaaccgtga gtacgcggca gcacgccagt acgcacatcc
    20641 agaccacgaa tcatatcttt ttcacgaccg tcgatacgcg gttcgcctgc cagtacgcgg
    20701 ctacgaacaa cgtttttctc gatagcgtgc agaatttcac ccagttcgtt ttcgtccagg
    20761 gtttcatctt cagcaagcag cgtcgcgatg gtttcagatt tgatgacatc aacctgcgca
    20821 taacgctctt gtttgtcggt gatgcggtaa gcatcgctca gacgagcttc agccagtgca
    20881 gcaacgcgcg cgttcagcgc ttcgtttacc ggctccggct gccagtccca acgcggttta
    20941 ccggcttctt tcaccagttc attgatgttc tgaataacaa cctgctgttg ttcatgaccg
    21001 aacactactg cgcccagcat ctggtcttcg ctcagcagtt cagcttcaga ttcaaccatc
    21061 agtacagcgg cttcagtacc cgcaacaacc agatccagtt tgctctcttt cagctcgtcc
    21121 tgagtcgggt tcagtacgta ctggtcattg atgtaaccta cgcgggcagc accaatcgga
    21181 ccattgaacg gaataccaga cagagacagt gctgcggaag caccaatcat cgcgacgata
    21241 tccgggttaa cctgcgggtt aacagaaacc acggtggcga taacctgaac ttcgttgacg
    21301 aagccttccg ggaacagcgg gcgaatcggg cggtcaatca gacgcgcgat cagggtttcg
    21361 ccttcgcttg ggcggccttc acgacggaag aagctacccg ggatacgacc agcagcgtag
    21421 gtacgctcct gatagttaac ggtcagtggg aagaagtcct gacctggctt ggcttttttc
    21481 tggccaacaa cggtaacgaa taccgcagtg tcatccatgc taaccataac agcggcagta
    21541 gcctgacgag ccatcatgcc ggtttccaga gtcacggtgt gttggccgta ctggaattta
    21601 cgaacgatcg gattaagcaa tgtaatatcc tttctctttc ttagacagta ccttacggca
    21661 ctggtgttaa tacccgatct tctgcgcatc ctcgcgacta atgacaaccc taacccagct
    21721 ctatgtgggt aaagcctctc attagccgcg cgaacctctg caacggaaga tcattcatag
    21781 caacaataca ttagtttcca gtgaattgct gccgtcagct tgaaaaaagg ggccactcag
    21841 gccccctttt ctgaaactcg caagaattag cgacgcagac ccaggcgctc gatgagctgg
    21901 gtgtaacgtg ctacgtcttt acgtttcagg tagtcgagca gtttacgacg ctgagaaacc
    21961 atgcgcagca gaccacgacg gctgtggtga tcttttttgt gctctgcaaa gtggccctgc
    22021 aggtggttga tctgtgcagt cagcagtgct acctgaactt cggtagaacc ggtgtcgttt
    22081 gcgtcacgac caaactcaga aacgatttta gctgttgctt cagtacttag agacatttta
    22141 aaactccaaa gtatatagaa tgaaaggacg ccgatctcta attcagcgat cccagtgtac
    22201 gttacgcaaa gtgttaaaca atttacgcga cgttaagcgg cagtattcta ctcgtagcga
    22261 cctgttatcg caagacggtt aacattacgc cgggtattca accaccaggc gacgaggcgc
    22321 gacgcggcct tcatcgtcaa tttcgcccat accgataaac ttgccgttct caccttcagt
    22381 aacgcgaacc agtccttcca gcggcgcacc ggatgtacga accgggttac catttttgaa
    22441 gtaaacagaa gacgttaacg gaagattcac caccgggtag tccgaagctg gactgtccat
    22501 tggcatcagt aatggatcaa gtaactccgc ggctggaata tcctgctgtt cggcttgttc
    22561 aaccagttca cgcagatgtt ccagggtcac catccgttca accggatatt tacttaccgc
    22621 cagacgacgc aggtaaataa catgcgcgcc acaaccgagt ttttcaccca ggtcatcaat
    22681 gatggtgcgg atataagtgc cttttgagca gtgaatttcc agctccagct cattgccttc
    22741 gtggcgaata aacagcaatt cataaacggt aatcgggcgc gcttcacgcg gaacttcaat
    22801 gccctgacgc gcatattcgt acagtttttt gccctggtat ttcagtgctg aatacatcga
    22861 tgggatctgt tcgatatcgc cacggaaagt atccagtgcc gccgccagtt gctctgcgct
    22921 aaaggttacc ggacgttctt caacgatttg tccatcggcg tcagacgtat cggtacgctg
    22981 tccaagacgc gcaatcaccc gatagcgttt gtcggagtcc agcagatact gggaaaactt
    23041 cgtcgcttcc ccgaggcaaa tcggcaacat gccagttgcc agcgggtcca gcgcgccggt
    23101 gtgtccggca cggttggcgt tatatatacg tttcactttt tgcagcgcat cgttgctgga
    23161 cataccctgc ggtttatcca gcaacaaaac gccgttaatg tcgcgaccgc gacgacgagg
    23221 acgactcatt agtcctcctt gctgtcgtcc gggttaacac gacgttcttc gtcatgtttg
    23281 accacgctgg tcaccaggtt tgacatgcgc atcccttcaa ccagcgagtt gtcgtagaag
    23341 aaggtcagtt ccggcacgat acgcaggcgc atcgctttcc ccagcaggct gcggatgaaa
    23401 ccagaagctt cctgcagcgc tttgatgccc gctttaaccg cgtcttcatc tttgtcgttg
    23461 aggaacgtta catatacttt ggcatacgcc aggtcgcgag acatttcgac accggaaacg
    23521 gtggtcatca tgcccagacg aggatcttta atttcacgct gcaggatgag agcgatctct
    23581 ttttgcattt cctgcgctac gcgctgcggg cgaccaaatt ctttcgccat aataaattct
    23641 cctgacaaaa aaggggccat agcccctttt aaaattaatc tcaggtggaa agggctgtgt
    23701 aggcctgata agacgcgcga gcgtcgcatc aggcaacggc tgccggatgc ggcgtaaaag
    23761 ccttatccgg cctacaaccc agacaacctt aagcgatggt acgttggatt tcgatgattt
    23821 cgaatacttc gatcacatcg ccagtgcgga cgtcgttgta gttcttaacg ccgataccac
    23881 attccatacc gttacggact tcgttaacgt catctttgaa gcggcgcagg gactccagct
    23941 cgccttcgta gataaccacg ttgtcgcgca gaacgcggat cgggttgtga cgtttaacca
    24001 caccttcggt aaccatacaa cctgcgatgg caccaaattt cggtgatttg aatacgtcac
    24061 gaacttccgc cagaccgata atctgttgtt tcagttccgg agacagcata ccgctcattg
    24121 ccgctttcac ttcgtcgatc aggttataga tgacggagta gtaacgcaga tccaggcttt
    24181 ccgcttcaat cactttacgt gcagaggcat cagcacgtac gttgaagcca accaggatgg
    24241 cattggacgc cgcagccagg gtagcgtcgg tttcggtgat accacctacg ccagaaccga
    24301 tgatcttcac tttaacttcg tcagtagaca gtttcagcaa ggagtcggag atagcttcca
    24361 cagaaccctg tacgtctgcc ttcaggacga tattcacttc gtgaacttcg ccttcggtca
    24421 tgttggcgaa catgttctcg agtttagatt tctgctgacg cgccagttta acttcgcgga
    24481 atttaccctg acgatagagt gcaacttcac gcgctttctt ctcgtcacgt acaacggtaa
    24541 cttcatcacc cgcagccgga acgccggaca ggccgaggat ttccaccgga atggacggac
    24601 ccgcttccag cacttcctga cccagttcgt tgcgcatcgc acgaacacga ccgtattcga
    24661 agccacacag aacgatatcg cccttgtgca gagtaccttc acgtaccaga acggtagcaa
    24721 ccggaccacg acctttatcg aggaaggatt cgataaccgc accgctcgcc atacctttac
    24781 gtaccgcttt cagttccaga acttccgcct gcagcaggat agcgtccagc agctcatcga
    24841 taccagtacc cgctttcgca gacacatgta cgaactggct ttcaccgccc cactcttccg
    24901 gcaggatgcc gtactgggag agttcgttct taacgcgatc cggatcggct tctggtttat
    24961 cgatcttgtt cactgcaacc accaccggaa cctgcgccgc tttcgcgtgc tggattgctt
    25021 cgatggtctg cggcattacg ccgtcgtcgg cagcaacaac caggactacg atgtccgttg
    25081 cctgcgcacc acgagcacgc attgaagtaa acgcggcgtg ccccggggtg tccaggaagg
    25141 tgatcatgcc gttttcagtt tcaacgtggt atgcaccaat gtgctgggta atgccgcccg
    25201 cttcgccaga ggccactttc gttgaacgaa tgtagtccag cagagaggtt ttaccgtggt
    25261 caacgtgacc catgatggtc acaaccggcg cgcgcggttc agccgctgca cccgtgtcac
    25321 ggtcgctcat taccgcttct tccaactcgt tttcacgacg caggataact ttgtggccca
    25381 tctcttcagc aaccagctgt gcggtttcct gatcgataac ctggttgatg gttgccattg
    25441 cgcccagttt catcatcgct ttgatgacct gagagccttt aaccgccatc ttgttcgcca
    25501 gttcgccaac ggtgatggtt tcgccgatca caacgtcacg gttaacggcc tgagcaggct
    25561 tctggaagcc ttgctgcagc gaagaacctt tacgttttcc gcccttaccg ccacgtactg
    25621 ctgcgcgtgc ttcttcacga tcagcttttg attcagcgtg tttgttgcct ttcttcggac
    25681 gcgcagcttt cgcgttacga ccacggccac ggccgccttc gacttcacga tcgctttcgt
    25741 cttctgcctg gcgagcatgt tgagaagtag tgacatgata atcgctggaa tcttcagtcg
    25801 gttccgcgtt atcagtccat ttgttttctt ccgccatacg acgtgcttct tcagcaacgc
    25861 gacgtgcttc ttcttcgagt ttacgacgcg cttcttcttc ggctttacgc ttgagctctg
    25921 cagcttcctg ctcacggcgg gctttttcag cctgggcgtt tttagtcata tcgtcttgtt
    25981 gattgctcac tttgtctttt tccgcagctt cacgtttcgc ttgttcagca gcttcacgct
    26041 tagcttgttc tgcggcctca cgttcagctt tttgttgcgc ctcgcgttta gccgattctt
    26101 ctgcctcacg acgggcttgc tcttccgctt cacgctgcgc ttgctcttcc gctgcaaggc
    26161 gttcagcctc ttgcggatcg cgtttcacaa aggtgcgttt cttgcggact tcgatttgta
    26221 ccgatttgct ttttccaccg gtaccaggaa tgttaagggt gctgcgtgtt ttacgttgca
    26281 gcgtcaattt gtccgggcct gaatttttct gattcaggtg gtcaatcaaa gtctgtttct
    26341 cttgtgcaga cacagagtcg tcagcagact tccggatacc tgcatcagca aattgctgta
    26401 ccaggcgttc cacggaggtc tgtcgctctg cggccagcgt tttaatcgtt acatctgtca
    26461 tgctgttcct tcctgctaca gtttattacg cttcgtcacc gaaccagcaa atattacggg
    26521 cagccataat cagtgctccg gctttttcgt cggtcaaccc ttcgatatca gccagatcat
    26581 caatgccctg ttcggcgaga tcttccagcg tacaaacgcc acgggcggcc agtttgaatg
    26641 ccaaatcacg atctacccct tcaaggttca gcagatcgtc agccggtttg ttatcaccga
    26701 ggctttcttc ctgggcctgt gcaatggtgg ccagtgcatt tttagcacgc tcgcgcagtg
    26761 cttcaacggt cggctcatca aggccttcga tttccaacag ctctttcatc ggcacatagg
    26821 ccagttcttc cagcgtcgag aaaccttctt ctaccagaac agtcgcgaag tcttcgtcga
    26881 tgtcgagata tttggtgaag gtgtcgatcg ctgcgtgcgc ttccgcctga tgcttagcct
    26941 gcaggtcgtc aacggtcatt acgttgagtt cccaaccgct cagctgcgaa gccagacgca
    27001 cgttctgacc gttacggcca atcgcctgcg ccaggttacc ggcttcaacg gcgatatcca
    27061 tggtgtgttt atcttcatcc accacgatag aagcaacgtc tgccggtgcc attgcgttaa
    27121 tcacaaactg cgccgggtta tcatcccaca ggacgatatc gatacgctcg ccgcccagtt
    27181 cagtagaaac cgcctgaaca cgcgcgccac gcatacctac gcaagcacct accggatcga
    27241 tacgtttatc gttggttttc accgcgattt tcgcacgaga acccgggtcg cgagccgctg
    27301 ctttaatttc aatcacttct tcgccgattt ctggcacttc aatacggaac agttcgatca
    27361 gcatttccgg cttggaacga gtgacgaaca gttgcgcgcc acgcgcttcc gggcgaacgg
    27421 aataaagcac gccacgaacg cggtcgccag ggcggaagtt ttcacgcggc agcatatctt
    27481 cgcgcaggat cacggcttca gcgttgttgc ccagatccag agagatgttg tcgcggttta
    27541 cttttttcac cacgccggtg atgatttcac cttcgtgttc acggaactga tcaaccacca
    27601 tcgcacgttc ggcttcacgc actttctgca cgataacctg ttttgccgtc tgggtggtga
    27661 tacggtcaaa ggtaacagac tcaatctgat cttcaacgta atcgcccagg ttcaggcttt
    27721 catcttcata acgtgcggct tcaagggtga tttccttggt cggctgggtg acttcatcaa
    27781 caactaacca gcgacggaag gtgtcaaaat caccgctttt gcgatcgatc tgtacgcgga
    27841 cgtcgatctc ttgttcatat tttttctttg ttgctgtcgc cagcgcgctt tccaatgctt
    27901 cgaaaatctt ctcgcgaggt agcgcctttt cattggatac ggcttcaact acagccaaaa
    27961 tttctttgtt catcgcgggc ttttcacctc atccagacta ttaaaagtgg ggaaccaggt
    28021 tcgccttctg gatattactc agcgcgaaca cttcatcttt accttcgacg gtaactgtga
    28081 tcatttcacc gtctaccgct ttgataacgc cctgccattt acgacggttt tgtaccgcca
    28141 tacggagaac cagagtcacc tcttctccga caaaacgggc gtagtgttca gccgtgaaca
    28201 gtgggcgatc gagacccggt gaggagactt ccaggttata agcaacggtg atgggatctt
    28261 caacatccag cacagcactt acctggtggc tcacatcagc acaatcatca acattgatgc
    28321 catcttcact atcaatatag atgcgcagtg tggatgtgcg accgcgaata aattcgatgc
    28381 caaccagttc aaaacccagg gcctcaactg gcgcagtaat catctctgtt aatttttgct
    28441 ctaatgtgga caagcccacc cccaagacat aaaaaaaggg cctaaagccc agttattctg
    28501 tagtcagata acaaaaaacc ccgataaatc ggggctttat ataactgaac cctataaccg
    28561 caactgcggt ctggagcact ttccagaagg attttttcaa atcccactac gaaggccgaa
    28621 gtcttcacag tatatttgaa aaaggactct aagggaaagt ggttgcgggg gccggatttg
    28681 aaccgacgac cttcgggtta tgagcccgac gagctaccag gctgctccac cccgcgcctg
    28741 aaacgtggca aattctactc gttttgggta aaaaatgcaa atactgctgg gatttggtgt
    28801 accgagacgg gacgtaaaat ctgcaggcat tatagtgatc cacgccacat tttgtcaacg
    28861 tttattgcta atcacgtgaa tgaatatcca gttcactttc atttgttgaa tacttttgcc
    28921 ttctcctgct ctcccttaag cgcattattt tacaaaaaac acactaaact cttcctgtct
    28981 ccgataaaag atgattaaat gaaaactcat ttattttgca taaaaattca gtgagagcgg
    29041 aaatccaggc tcatcatcag ttaattaagc agggtgttat tttatgacga cgattctcaa
    29101 gcatctcccg gtaggtcaac gtattggtat cgctttttcc ggcggtctgg acaccagtgc
    29161 cgcactgctg tggatgcgac aaaagggagc ggttccttat gcatatactg caaacctggg
    29221 ccagccagac gaagaggatt atgatgcgat ccctcgtcgt gccatggaat acggcgcgga
    29281 gaacgcacgt ctgatcgact gccgcaaaca actggtggcc gaaggtattg ccgctattca
    29341 gtgtggcgca tttcataaca ccaccggcgg cctgacctat ttcaacacaa cgccgctggg
    29401 ccgcgccgtg actggtacca tgctggttgc tgcgatgaaa gaagatggcg tgaatatctg
    29461 gggtgacggt agcacctata aaggaaacga tatcgaacgt ttctatcgtt atggtctgtt
    29521 gaccaatgct gaactgcaga tttacaaacc gtggcttgat actgacttta ttgatgaact
    29581 gggcggccgt catgagatgt ctgaatttat gattgcctgc ggtttcgact acaaaatgtc
    29641 tgtcgaaaaa gcctactcca cagactccaa catgcttggt gcaacgcatg aagcgaagga
    29701 tctggaatac ctcaactcca gcgtcaaaat cgtcaacccg attatgggcg tgaaattctg
    29761 ggatgagagc gtgaagatcc cggcagaaga agtcacagta cgctttgagc aaggtcatcc
    29821 ggtggcgctg aacggtaaaa cctttagcga cgacgtagaa atgatgctgg aagctaaccg
    29881 catcggcggt cgtcacggcc tgggcatgag cgaccagatt gaaaaccgta tcatcgaagc
    29941 gaaaagccgt ggtatttacg aagctccggg gatggcactg ctgcacattg cgtatgaacg
    30001 cctgttgacc ggtattcaca acgaagacac cattgagcag tatcacgcgc atggtcgtca
    30061 gttgggccgt ctgctgtacc aagggcgttg gtttgactcc caggcgctga tgctgcgtga
    30121 ctctctgcaa cgctgggttg ccagccagat cactggtgaa gttactctgg agctgcgccg
    30181 cgggaacgat tattcaatcc tgaataccgt ctcagagaac ctgacctaca agccagagcg
    30241 tctgacgatg gaaaaaggcg actcggtgtt ctcgccagat gatcgtatcg gtcaattgac
    30301 catgcgtaac ctggatatca ccgatacccg cgagaaactt ttcggttatg ccaaaactgg
    30361 cctcctctcc tcctctgccg cttcaggcgt gccgcaggtg gagaatctgg aaaataaagg
    30421 ccagtaattc gacatcaacc ctgcccttca ggggtggggt taccttgctt atcctctgta
    30481 aactcctttc tgagcatcac ataatcgaaa acatcatggt ttgcatccac aacccgtgag
    30541 tctcctttat agtgtgcaat cacttcctcc agcgttttcg gttgttccgg ctttgttacc
    30601 cccatccacg cattaatcag gtaattattg taagctgtgg aaaagatgtt gttttccgtt
    30661 gtgcgatcca cgccatcccc aagaacgggg ctataccaga taaacatcgg gacatgatat
    30721 gcctgctggc tagcctccct gcctccatga aaatagacgt tcttcttcgt agggtcacgt
    30781 tccaggccgt ggtcggcaaa atacatgacc gaggcgcggc gatcttttaa taattcaaaa
    30841 atctgaccta gcaaactatc tgtgtaatga atggagttgt catagcaggc atcctgatcg
    30901 tcctgcggtt gaaacacggc gctggattgc ggataggcgc tacaagccgg ttcatggctt
    30961 ccatttaaat gaagaacaat cagtttcttt tgctgcgtct tttgctttaa cgcttgcgat
    31021 aaatgcggca acaacaattc atcaaatcct ctgacataaa ctgtttccat ggcgcgcatg
    31081 gcgatactgg taactgctgt accattctgc cgaaaagcgg attgcgagct tagccagaaa
    31141 gtctgaaatc ctgcttgatt agccatatta ataatgttgt ccgggtaatt atgaatgtca
    31201 tgactcaaaa cggaatcagc agttaaagaa agaggaaccg acagcgcagt gtaaggtgca
    31261 ccgcttattg cttgattaaa cagtttgatc tgttttcttt gtgcttcgac ttgcggtgtc
    31321 gtagagcgtg tatatccata caaagacata ttgtcgacac gtacagactc ccccacaatc
    31381 aacacgtagg tatcaatacc tgtatccctg actgataatt gaaaatacgg caccgtgttt
    31441 gcaattgaga gtaatctttg atgctctttc gctgctaaag caaaataatt gagattgaaa
    31501 aacggtgtat aggtagcaaa tcgcgacgct agtatatatg gactgaacgc ttttttattt
    31561 tttgcatctt tataagcaaa ttgacaagcg gaaaataaac tgcccgaaat gacaatcagc
    31621 aataatattc ctgtcacttt tttcgtcggc aaggaaacat catattttat tattactgca
    31681 aaaaaaagaa gggataaaaa ggcaaaagca catagataag ggatatacat ccccagcatt
    31741 ttgacaactt catccggatc actctgcagc acgctaatcg caaatccatc attgaaagtt
    31801 gtaccaaagg tacaccatga atagaggcta atactcatgt ctgccgctgt cagtaacaca
    31861 aatggtgcgg caacaagcag gcgaagcgga agggatgata tacgtttaac aaccaggatg
    31921 gtcagaacga aaaacagcaa tcgctgaacc atatgcgctc cggaagaggc gactaagttc
    31981 gtaataccaa aaacaataac acaaagatac aacaaccaat gagatttaaa acttctaaca
    32041 aatttgttga atactgtcat ccagaaatcc aagttcacaa ccaatataaa caattatcaa
    32101 caatgttcaa ttttatatca gacatgtttt tcaactctga tccatatcag accgaaacac
    32161 tgttagtttt ttctttttaa atgatgcggc cattgttctc agtaattaaa taacattatc
    32221 aatgcgtctc taaacattac gcagaacgta gccatcagta gttatactga caggagcagg
    32281 atggtttatc gagaagtaaa catatccgta ttcgtaccgg aactggagtt gacacaataa
    32341 agtgccaatt atgtcagcag aagggaaaag tttataacta aggcgtaccg ataccatcgt
    32401 ttcaaagtac cagctacgag taaagcaact ggacgagata cagatacctg acaaccattc
    32461 ctcagaccag gaccaaaacg aaaaaagacg cttttcagcg tctcttttct ggaatttggt
    32521 accgaggacg ggacttgaac ccgtaagccc tattgggcac taccacctca aggtagcgtg
    32581 tctaccaatt ccaccacctc ggcacggata ctacttttta gttcgggata tcgctggtcg
    32641 gcttagccgg agcagctggc tgagtttgtt cggttttcgc cggcgcactc aggttttccc
    32701 attcgctacc tttattggtt ttattgctat tgatgttacc cagcaccaga ctgatgatga
    32761 agaataacgt tgccagcagc gccgtcatgc gggtcatgaa gttaccagaa ccacttgaac
    32821 caaacagcgt agcggaagcg cctgctccga aggaggctcc catatcagcg cctttacctt
    32881 gctgcagcat gatcagacca acaaggccaa ttgccacaat aaggaaaact actaaaagag
    32941 cttcgtacat aatcaacctg ttccttgcgg agttgccgcg taccaatgct tcaaccaata
    33001 aagcgggagt ttttaatctt tcccactgaa gcgggtgtga atactaacca aagcgaatga
    33061 ccttcgcaag ggcaatttta ttgcattgta tcaactgcgg aaaaaaacag caaaaccgat
    33121 gtgttcgccg agaaaaaagg cgacaatcgc cgccttttta gccagttatc taacgcttta
    33181 aacggctttt actgcatcgg cgatacggtg tgcaaattca gttacctgcg cttcgtcttc
    33241 accttctacc atcacgcgaa ttaacggctc ggtgccagat ttacgcagca aaacgcgtcc
    33301 acggctaccc agcgcagctt caacctctgc ggtcacggct ttaactgact catgctcaag
    33361 cggatcgccg ctacctgcgg tgtaacgcac gttaaccaga atctgcggga acattttcat
    33421 gccgctgcaa aggtcgtgca ggctcatatg gttacgtgcc atcgccgcca gcacctgcaa
    33481 accagcaacg atgccgtcac cggtagtggt tttatccagc aggatcacat gaccggaatt
    33541 ctctgcaccg atacgccagc ctttctcctg cattttttcc agtacgtagc ggtcacccac
    33601 tttcgcgcgc gcaaatggaa ttcctaactg tttcagcgcc agttcaagcc ccatgttgct
    33661 catcaatgta cccacagcgc caccacgcag ctggccctga cgaagacctt cacgcgcgat
    33721 gatatacatg atctgatcgc catcgacttt attgccttca tggtcaacca taatcacgcg
    33781 atcgccgtcg ccgtcgaagg caataccgag atccgctttt tcagccagca cacgagcctg
    33841 gagcgcgcga acgtcggtag cccccacttc ggcattgatg tttacaccgt tcggctcaca
    33901 accgatagcg ataacgttcg ctcccagttc acgcagcacg ttcggcgcga tgtgataagt
    33961 cgcaccgttt gcacaatcca ccacaatctt cagttcactg aggctaagtt cgtttgggaa
    34021 cgtggctttg caaaactcga tatagcgacc cgcagcatca acgatacggc tggctttacc
    34081 cagttctgcc gaatcaacgc agctgatctc tttttccatt tccgcttcga tggcttcttc
    34141 aactgcatcc ggtagtttgg tgccgtcgat agagaaaaat ttaatgccat tatcgtagaa
    34201 cgggttatgc gatgcagata tcacaattcc ggcctctgcg cggaaggtac gcgtcagata
    34261 agccacggcc ggtgttggca tcgggccagt gaagagtgcg gaaaggcccg cagccgccag
    34321 acccgcttca agtgctgact ccagcatata gccagaaata cgcgtgtctt taccaataat
    34381 gatcttacgg gagccgtggc gcgccagcac tttacccgcg gcccaaccca gcttaagcac
    34441 aaaatcaggg gtgatcggcg catcccctac acgaccacga atcccatcgg taccgaaata
    34501 tttacgatta ctcatagcgt ttgttttcct ttgcagacag agtggcttcc accacccgca
    34561 tcgcttctac ggtttctttg acgtcatgaa cacgaatgat atgcgcgcct tgcatagcgg
    34621 caatcaccgc acaagccaga ctaccgctca gacgctcgga cggccccacg ttcagcagct
    34681 gcccaatcat cgattttcgt gacataccca ccaacagcgg caggttgaaa tggtgaaatt
    34741 cagccaggcg cgccagtaat gaatagttat gggagagatt tttaccgaaa ccgaatccgg
    34801 ggtcgagcaa caatttctct tttgcgatac ccgcctgctc gcaacgtgct atttgctcaa
    34861 taaaatagcg attcacttct gcaaagacat cgtcatactt cggggcttcc tgcatggtct
    34921 ttggatttcc ctgcatatgc atcagacaaa ccggtaaacc ggtttctgca gctgcctcca
    34981 gagcccctgg ttcggaaagg gagcggatat cgttaataat gtgagcgcca actttcgctg
    35041 actcacggat gacttctggt ttggatgtat cgaccgagat ccagacttcg aagcgttgag
    35101 caattgcctc aaccacagga ataacacgtt gcaactcttc ttcaacgcta acttccgccg
    35161 ctcctgggcg cgtggactcg ccaccaacgt caatgatcgt cgcgccagcg ttgatcatca
    35221 gattcgcatg tttcaccgca tctatcagcg agttatgcgt gccgccatcc gagaaggaat
    35281 caggcgtgac gttgaggatc cccattacgt gaggatggct aaggtccagt gaagtgccct
    35341 gggcaaagag tttcatggtg ttatccctgg tatgaattga taagaaaaaa ccccggagca
    35401 cgccccgggg ttttcggtac aaatacagtc atctgatgcg ggaacttact tgtcgcctaa
    35461 ctgctctgac atggtgttac ccgggttcgg cgtacgcggt tcatcaaccg gacgcggagc
    35521 ctttgggcta ccattgtcgc cagaattgtt agaagcgcct ggttcttccc agcccgctgg
    35581 cggacgtaca tcgcgacgtg ccatcaggtc atcaatctgc ggtgcgtcga tagtctcata
    35641 tttcatgaga gcatctttca tcgcatgcag aatatccata ttgtcggtca gaagctgacg
    35701 cgcacgatta tagttacgct cgatcagtgc tttcacttcc tggtcgatga tacgtgcagt
    35761 ttcatcggac atatgtttcg ctttcgctac gctacggccg aggaacactt caccttcttc
    35821 ttccgcgtac agcagtggcc ccaatttctc agagaagccc cactgagtca ccatgttacg
    35881 tgccagattg gtcgcaactt taatatcgtt ggatgcaccg gtagaaacat gttccggccc
    35941 gtagatgatc tcttctgcca gacgaccacc gtacagcgta gaaatctggc tttccagttt
    36001 ctgacggctg gcgctgattg cgtcgccctc aggcaagaag aaagtcacac ccagcgcacg
    36061 accgcgtggg ataatcgtca ctttgtgcac cggatcgtgt tccggcacca ggcgaccgat
    36121 aatcgcgtga cccgcttcgt ggtaagccgt cgattctttc tgcgcttccg tcatcaccat
    36181 ggagcgacgt tccgcaccca tcatgatttt gtctttcgct ttctcgaact caaccatcga
    36241 cacaacgcgt ttgttgccac gagcagcgaa cagtgccgct tcgtttacca ggttcgccag
    36301 gtcagcaccg gagaaaccag gagtaccacg ggcaatgatt gccgcgtcga tatcgggtgc
    36361 caatggtacg cgacgcatgt gaactttcag gatctgctca cgaccgcgaa catctggcaa
    36421 gccaaccaca acctgacggt cgaaacggcc aggacgcagc agcgccgggt cgagaacgtc
    36481 cggacggtta gttgcggcga taacgatgat accttcgtta ccttcgaagc catccatctc
    36541 aaccagcatc tggttcagag tctgttcacg ttcatcgtga ccaccgccca gacctgcgcc
    36601 acgctggcgg cctacggcgt cgatttcatc gataaagatg atacacggtg ccgctttctt
    36661 cgcctgttcg aacatgtcac gaacacggga tgcacccaca ccgacgaaca tttctacgaa
    36721 gtcagaaccg gagatagtaa agaacggaac tttcgcttcg cctgcaatcg ctttcgccag
    36781 cagcgtttta ccggtacccg gaggaccgac catcaggacg cctttcggga tcttaccgcc
    36841 gagtttctgg aagcggctcg gctcgcgcag atactcaacc agttcagcaa cttcttcttt
    36901 tgcttcgtcg cagcccgcaa cgtcagcaaa ggtcgttttg atctgatctt ccgtcagcat
    36961 gcgcgctttg ctcttaccaa acgacatggc acctttgcca ccgccgccct gcatttgacg
    37021 catgaagaag atccagacac caatcagcaa cagcatcggg aaccaggaga tgaagataga
    37081 agccagcagg cttggttctt caggcggttc accgacaacc ttgacgttct tggtcaacag
    37141 gttatccagt aatttcggat cctgaaccgg aatgtaagtg gtataacggt tactatcttt
    37201 cttggtaacg ttgatttcac gtccgttgat acgcgcttca cgaacctggt cgttattgac
    37261 ctcttgtagg aaggtagagt aatccacctt acggccatta gactcgctgg gcccaaagct
    37321 ctggaatact gacatcagca caacggcaat gaccagccag agtattaggt ttttcgccat
    37381 gtcactcaag ggattaacct cttattacaa ctgtgttaaa aacagcgtca ggatactcta
    37441 tatccagcat ctttcaaact ttcgtctgaa atctcccggt tagggtttac gcccggtcgc
    37501 tacaatatac acttcccgcg aacgtgcgcg agaagagtcc ggcttacgaa ctttgacctt
    37561 cgtaaacagg gagcgaattt cccttagata ctcatcgaaa ccttcgccct ggaacacctt
    37621 cactacaaaa cttccacctg gcgctaatac atcacgacac atttctagcg ccagttccac
    37681 cagatacatg gcacggggga tatccaccgc cggtgttccg ctcatgtttg gtgccatatc
    37741 ggacatgaca acctggactt tgctgtcgcc aacgcgctcc agcagtgctt tcatcaccag
    37801 ttcatcacga aaatcgccct gaagaaagtc cacaccaacg ataggatcca taggtaaaag
    37861 atcgcaagcg atgatgcggc ctttgccgcc aatttgggtg accacatatt gtgaccaacc
    37921 acccggagca gcaccaaggt cgacaaccgt cattcccggt ttaaagagtt tgtcactttg
    37981 ctgtatttca tcaagtttaa accaggcacg ggaacgtaac ccctttttct gtgcctgttg
    38041 aacatattta tcgctaaagt gttcctgaag ccagcggctg gagctggcag aacgcttctt
    38101 acctgtcatt taactttccc attgggataa ctcatcgtaa ccaattgcgt aaatttttac
    38161 gcgcctattt ggcgatatat gggagatggc ggtagaatga cccgttttca atcccaacgt
    38221 aagcaaaaaa tacgatgaat ctgagtacta aacaaaaaca gcacctgaaa ggtctggcac
    38281 atccgctcaa gccagttgtt ctgcttggca gtaatggttt gaccgaaggg gtgctggccg
    38341 agattgaaca agcgttagag caccatgaac tcatcaaggt gaaaatcgcc accgaagatc
    38401 gcgaaactaa aaccttgatc gtggaagcta tcgtacgcga aaccggcgcc tgtaatgtac
    38461 aggtcatcgg taaaacgctg gtgctttatc gcccaactaa agaacgtaaa atctcgctgc
    38521 cacgctaaga ttatcctaaa gttacacaca tttgctgtgt aaaacgaggg gttttccgca
    38581 ggcaggagag caaaatgcca cgctctgttc gttgataaaa ggccgcatag cggccttttt
    38641 cctttcttta caatacatca acatcttgag tattgggtaa ttcttacagg tattccacct
    38701 taattacttc aaattctact tcgccgcccg gcgttttgat gaccacaaca tcatcttctt
    38761 ctttgccgat caggccacga gcaataggcg agtttacaga aatcaggttt tgtttaaagt
    38821 cagcttcgtc atcgccaacg atgcgataag tctgttcttc gtcagaatcc agattcagca
    38881 ccgttacggt agcaccaaaa ataacgcgcc cattgttggg cattttggtg acatcaatca
    38941 cctgcgcgtt cgacagcttg gcttcgatgt ctttgatacg gccttcgcag aagccctgct
    39001 gttcacgagc tgcgtggtat tcggcgtttt ctttcaggtc gccatgctca cgcgcttccg
    39061 cgatagcagc aatgatttca ggacggcgca cagatttcag aaaatccagc tcttcgcgta
    39121 atttttcagc gccgcgtaag gtcatcggaa tagcttgcat ttgttatacc tcttgaatat
    39181 tcctgatggg gcaagtcttc acccaccccg gctgttcggc ctgcccggca taactcctca
    39241 ccgggaccag aagcaaaaaa ataccgaccc gggtacaagt cccaggtcag ctacaattca
    39301 cattttgata gtcattttac cctgaagttc ccgaagggtc atcgtttact ttatagggcg
    39361 ttgcgccgta gtatgacggc tcgattccag gttgttagcg cgagattatg cgattttcca
    39421 gatttatcat cggattgacc agctgtatag cgttcagtgt tcaggccgca aatgttgatg
    39481 agtacattac tcaactcccc gctggtgcca accttgccct gatggtgcaa aaagtcggcg
    39541 cgtcggcccc cgctattgat taccacagtc agcagatggc gctgcccgcc agtacgcaga
    39601 aagtgattac tgcactggcg gcgttgattc aactcggccc cgattttcgt tttaccacga
    39661 cgcttgaaac caaaggcaat gtggaaaacg gcgtacttaa gggtgactta gtggcgcgat
    39721 ttggtgccga tccgacgtta agacgtcagg atattcgcaa tatggtcgcg actttgaaaa
    39781 aatctggcgt caaccaaatc gatggcaatg tgttgataga tacctccatt ttcgccagcc
    39841 acgataaagc ccccggctgg ccatggaatg acatgacaca atgctttagc gctccgcctg
    39901 ccgccgccat agttgaccgc aactgtttct ccgtctcgct ctatagtgcc ccaaaacctg
    39961 gtgatatggc ttttatacgc gtggcatctt attaccccgt tacgatgttc agccaggtac
    40021 gcaccctccc ccgtggttct gccgaagcgc aatactgcga gctggatgtg gtgccaggcg
    40081 acctgaaccg ctttacgctg accggatgcc tgccacaacg ttctgagccg ctcccgttgg
    40141 cttttgccgt gcaggatggt gccagctatg ctggagcaat tctgaaagat gagttaaaac
    40201 aggcaggtat cacctggagc ggaacactgc tgcgtcagac gcaggttaac gaacccggaa
    40261 cggtagttgc cagtaaacag tcggccccgt tgcacgatct gcttaagatt atgctgaaaa
    40321 agtcggacaa catgattgcc gatacggttt tccgcatgat cggccatgcg cgcttcaatg
    40381 tgcctggaac atggcgggcc gggtcggacg ccgtgcgtca gatcctgcgc cagcaagccg
    40441 gtgtcgatat tggaaatacc attattgccg atggttcagg gctttcgcgg cataacctga
    40501 ttgctcccgc caccatgatg caggtgctgc aatacattgc ccaacacgac aatgaactta
    40561 actttatctc tatgctgccg ctggcgggct atgacggctc tttgcagtac cgtgcaggtc
    40621 tgcatcaggc gggcgtggat ggaaaagtct cagcgaaaac cggttcgttg cagggggtat
    40681 ataacctggc gggattcatt accacagcga gcgggcaacg aatggcgttt gtgcaatatc
    40741 tttctggcta tgcagtagaa ccggcggatc agcgtaatcg ccgtattccg ttggtacgtt
    40801 ttgaaagccg tttgtataaa gatatttacc agaacaatta gtctaaagag aaaccccggc
    40861 acatggccgg ggttttgctg attaacgctt gtaaatgaac tcaacgcctt cttcgtcgtc
    40921 ttcgtcccag tcgtcatccc agtcttcatc atcctcttca gcaatctctt caagctgctg
    40981 gcgatgataa tcatcccaca tgaattcgac tttctctggc tgtttcgctt cttcagcctg
    41041 cacgaccggg ttttcaatga taaaggtcat cacatcccag cagagatctt tcacgcccag
    41101 tccgctcgcc gcagagatca gataatattt atcttcccaa cccagcgcct cagcgatcgc
    41161 tttcgctttc tcttcggctt ctgccttatc cagcaggtcg atcttgttga agactaacca
    41221 acgcggtttc gccgccagat cctggctgta tttttccagc tcgctgataa taatacgcgc
    41281 gttttcaacc ggatcggtgc cgtcaatcgg atcgatatcg atgaggtgca acagaacgcg
    41341 gcaacgctcc aggtgcttca ggaagcgaat gcccagacct gcgccttctg cagcgccttc
    41401 aatcagtcct ggaatatcgg caacaacgaa gctcttttcg ttgtccattc gtaccacacc
    41461 aagacttggc accagagtgg taaacggata atccgccact ttcggtttag ccgccgatac
    41521 cgcgcgaata aaggtcgatt tacccgcgtt tggcatcccc aacataccga cgtcagccag
    41581 cagcatcagc tccagcagca gctcacgctt atcacccggc gtaccgttgg ttttctgccg
    41641 tggtgtacgg ttaacggacg atttgaaacg ggtattgccc agaccgtgcc agccgccctt
    41701 agcaaccagc agacgctgac cgtgtttggt catatcgccc atggtttcac cggtaccctg
    41761 gtcgattaca cgcgtaccta ccggtacttt aatcgtgacg tctttaccgc gcttaccggt
    41821 acagtctcgg cttgcaccgt tctgaccgcg ctctgcacgg aaagattttt caaaacgata
    41881 atcgataagc gtgttcaggt tctcgtcggc ttccatccat acgtcaccac catcaccgcc
    41941 gtcgccgcca tccgggccgc ctttcggaat atacttttcg cggcggaagc tcacgcaacc
    42001 attaccgcca tcacctgcaa cgaccagaat cgatgcctca tcaacaaact tcattttatt
    42061 ctccgtaaat cattcgcctg cgcggggttg cgataccacc gttgtatgct tacgtaatcc
    42121 gccccaaata cgatgaccaa tggcggaata catcgcgccc gcaaccacga caaacgcacc
    42181 gagataacct aaaaggttta acatcggtct ggcgaagaaa tcgggccagg ccagtgataa
    42241 aagatctgaa aaaaacagcg taaacagtgg ggtgagcgtg atgatcgcgc tcacctgcgc
    42301 tgcctgccag cgagccatcg cttccgccag ggcgccatat cctaccaatg tattcagtcc
    42361 gcaaaaaatt aaacatgcga gctgccagtg gctaagctgc gctatcactc caggatttgc
    42421 cagagggaag agcgcaattg tacataaagt gtacagtaaa aacaggatct gcggtgaggc
    42481 cagacgacgc aataaaacct tttgcgccac gccataactc acccaaaccg tcgccgcacc
    42541 gaccccaaag ataactcccc aggtgtaatc ggtgagcttt gtaaatatct cgaccagact
    42601 ggtgttaaaa aacatcacca ggccgctcag gagcatcaat gcccctacaa cctgagtact
    42661 gcgcattttc tctttcagga taaatacgct ggcaaccatc atgccaactg gcgagagttg
    42721 cccaattacc tgcgaagcgg tcggactcag gtattgcaag gatgagctga acaggatgaa
    42781 gttcccaaac agcccggcag tcgccactgc taacaaaatc aaccagcgtg gcttacgaaa
    42841 cacgcgtaat ggcggcaacc gcttcttcac cgcaagaatg gcaccaaggc caatactcgc
    42901 catcaagaaa cggtaaaaca cgattgtcgg aggttccatc acctccagca cctgcttcat
    42961 tgcgattggc aacgcccccc agcaaattgc tgtggtgagc gccaaaagaa tgccaatgcc
    43021 tgcctgctgc ttcataccgt attccctgca aagcgcatat gcggattaac gtcgcaccat
    43081 gcgcattttt accggttatc gaatgtaaaa agccccgcaa cgtgttgcgg ggctttcatc
    43141 cgttaccggg acgcgaaaaa cttattcagc ttcgatgctg ataaatttac ggtttttcgg
    43201 gcctttaact tcgaatttca ctttaccgtc tgctttagca aacagagtgt ggtcacgacc
    43261 gcaacctacg ttagcgccag cgtggaattt ggtaccacgt tgacgaacga tgatgctacc
    43321 cgccagaacg gattcgccac cgaaacgctt aacgcccagg cgtttagctt ctgaatcgcg
    43381 accgttacgt gtggagccgc cagccttttt atgtgccatt tgaaatctct cctcaggtct
    43441 taggcgctga tgccagtaat tttcacatca gtgaaccact gacgatggcc ctgctgctta
    43501 cgatagtgtt tacgacgacg aaacttaacg attttaactt tctcgccacg accgtgagca
    43561 acaacttcag ctttgattac gccgccatca acgaaaggaa cgccgatttt gacttcttca
    43621 ccgtttgcga tcatcagcac ttcagcgaac tcaacagttt cgccagttgc gatgtccagc
    43681 ttttccaggc gaacggtctg accttcgctt actcggtgtt gtttaccacc actttggaaa
    43741 accgcgtaca tataaaactc cgcttccgcg cacacctttt caatgattca gagtgcgcta
    43801 taaatattca caatagggcg cgaatattac gcaaaacgca cgcctttgac aagtgctaca
    43861 gtcaatacac gaagaaaaaa acacaacttg tacggtaacg tttatctgtg ccattttttc
    43921 agtacaatca ccctatattc ctaaccataa accctaagtt gcctttgttc acagtaaggt
    43981 aatcggggcg aaaagcccgg cttttgcgat gaatttagaa aaaatcaatg agttaaccgc
    44041 gcaagatatg gcgggtgtta atgcggcaat ccttgagcag cttaattccg acgtccaact
    44101 gatcaatcag ttaggctatt acatcgtcag cggcggcggt aaacgtattc gtccgatgat
    44161 tgctgtactg gctgcacgag ctgttggcta tgagggaaat gcgcatgtca ccattgctgc
    44221 cctgatcgag tttatccaca cggcgactct gctacacgac gacgttgtgg atgaatcaga
    44281 tatgcgcagg ggtaaagcta ccgccaacgc cgcatttggc aatgccgcca gcgtgctggt
    44341 aggcgatttt atttataccc gcgcgttcca gatgatgacc agcctcggtt cactcaaagt
    44401 gctggaagtc atgtcagaag ccgtaaacgt catcgcagaa ggtgaagttc tgcaactgat
    44461 gaacgttaac gatccggaca tcactgaaga aaactacatg cgcgttatct atagcaaaac
    44521 cgcgcgtctg tttgaggctg ccgcgcagtg ttccgggatt ctggctggct gtacgccgga
    44581 ggaggagaaa ggcctgcagg attatgggcg ctatctcggc actgctttcc agttgatcga
    44641 cgatttactc gattacaatg ccgatggcga acagttaggt aaaaacgtcg gcgacgatct
    44701 gaacgaaggt aaaccgacgc tgccgctgct gcatgcaatg catcatggca caccagaaca
    44761 ggcacagatg attcgtaccg ccatcgaaca gggtaacggt cgccatcttc tggaaccggt
    44821 tctggaagca atgaacgctt gtggatctct tgaatggacg cgtcagcgtg ccgaggaaga
    44881 agcagacaaa gccatcgcag cgttacaggt gctcccggac accccttggc gagaagcact
    44941 catcggcctc gcgcacatcg ctgttcaacg cgatcgttaa tcccctcccc tcatcccgcg
    45001 cagcgcgcgg gatgattcca gtaaattcca taaaaacact tattcagctc tataacacct
    45061 gaaaaatagg gccgtacatc tttatatatt ctgaatattc acactcttta caggaacttt
    45121 ttagagcaat aggccatcag gagtatagtg atgctcgaca gaagaagtgt tctgaatgaa
    45181 agcgactaac ttaaggagtg aggaaagatg gaaagtaatt tcattgactg gcatcccgct
    45241 gacatcattg cgggtttgcg caagaaggga acgtcaatgg cggcggaatc tcgcagaaat
    45301 ggtttgagtt cctcaacgct ggcgaacgca ttatcgcgcc cctggccgaa aggagagatg
    45361 attattgcga aagccctggg aactgacccc tggattatct ggccatctcg ctaccatgat
    45421 ccgcagaccc atgagtttat cgacagaacg cagttgatgc gtagctacac taaaccgaaa
    45481 aaatgagtgg tctggcggta gccccgcgaa cggggctgcc agctctcaga caattactcg
    45541 cctttcacac gctcaatatt tgcacctaaa gcgcgcagtt tgtcttcaat gcgttcgtag
    45601 ccacgatcga tgtgataaat acgatcaact accgtcgtcc cttccgcaat acagccagcc
    45661 agcaccaggc ttgctgatgc acgtagatcg gttgccataa cctgtgcgcc agaaagtttt
    45721 tcaacaccgt gacaaataac ggtattgctt tcgatttcgg cgtgcgcgcc catacggctc
    45781 agctctggca catgcataaa gcggttttca aagaccgttt cggtgatgaa gccggtccct
    45841 tctgccacca ggttcaacag tgtgaactgg gcctgcatat cggtcgggaa tgccggatgc
    45901 ggcgcagtac gtacgttaac agccttcgga cgtttgccat gcatatccag gctaatccag
    45961 tcttcgccga cttcgatgtc cgctccagcg tcacgcagtt tcgccagcac ggcgtccaga
    46021 gtatctggct gcgcgttacg gcagataatt ttgccgcgag agatcgccgc cgccaccagg
    46081 aaagtaccgg tttcgatacg atccggtagc acgcgataga caccgccgcc taaacgttcc
    46141 acaccttcga tgacgatacg atcggtgccc tgaccgctaa ttttcgcacc cagcgtaatc
    46201 aggaagttcg cggtatcgac gatttccggt tcacgcgctg cgttttcaat aatcgtggtg
    46261 ccttcggcca gggttgcagc acacatgata gtcaccgttg cgccaacgct gactttatcc
    46321 atcacgatat gtgcgccttt cagacgacca tcgacggaag ctttaacgta accttcttcc
    46381 agtttgatgg tcgcgcctaa ttgttcgagg ccagaaatgt gtagatcaac cggacgtgca
    46441 ccaatcgtac aaccgccagg cagtgaaact tgcccctgac caaagcgcgc taccagcggc
    46501 cccagcgccc agatagaagc acgcatggtt ttaaccagat cgtaaggtgc gcagaataca
    46561 ttaacgtcgc gggcatcaat atgcacagaa ccattacgtt ctactttcgc acccagctgg
    46621 cttagcagct tcattgatgt atcgacgtct tttagtttcg ggacgttctg gatctctacc
    46681 ggttcttccg ccagtagtgc ggcaaaaagg ataggcagag cagcattttt agcgccggaa
    46741 attgtgactt cgccctggag cttcgttggc ccctgaacac gaaatttatc catttagttt
    46801 gttctcagtt aacaattcat atccgctacc ggcgaatcgc ccatagctca aaagccgttc
    46861 agtttgcgat cgcgcgccca ctccgcaggg gtatacgctt tgatcgacac agcatgaatg
    46921 cggttatccg caatatattc catcagcggg ccatagaccg tctgctgttt tttaacccga
    46981 ctcatgccgt caaacaactc acccacggca ataacctgaa agtggctgcc atcgccggaa
    47041 acgtggactt cctggaggga gagagcgttc atcaacacgc tctgaatttc attattttcc
    47101 atgggatctt caatcatcag ttaataaacc agcgaaacat cttagagcaa agttgcgctg
    47161 gcataaataa gcaaaaagcc tcgctgataa atcagacaag gctcgacttg caggcaggtt
    47221 tgccggacag gcggttaacg ccatatccgg cctgaaaaaa tttaacgagg cagaacatca
    47281 gcaggcaaat tatacaattt cgccagggta tacactttgt cgtttacccc ctgaagcgtc
    47341 acgttgttgc cctgcttttt cgccagatcg ataagatgga gcagcagtgc cagtccaccc
    47401 gtatccacgc gggacacacg gctaagatcg atgcaggtaa tccccttcac cgcttcctca
    47461 cgcatttccc aaagcggtag cagaacgtcc tgatccagct ctccggatag cgccagcgtg
    47521 tcacccgtct gcatccagct cagtgactcg ctcattattt tttctcttcc agagtgattt
    47581 tctgttgaga aatcgatttc agttgtgcag tcaggccgtc gatacctttg gtacgcagca
    47641 gtgttcccca ctcgttttgt ttggtggtga tcatactgac gccttcagca atcatgtcgt
    47701 aagcctgcca gttgcccgtc tgggagtttt tacgccactg gaagtccaga cgcaccggcg
    47761 ggcggccatt cgggtcaata atggtaacgc gaataggcac aatggtttta tcgcccagcg
    47821 gctgttctgg cgcaatctga taggtttgac cgtgatacat cgccagcgcc tgaccgtaag
    47881 cctgcttcag gtactcacgg aaagcggcaa agtaagcatc acgttgagca ggggtcgcac
    47941 tcttgtaata ctggcccagc accagcgcac cggcgtattt cacctgtacg tatggcagca
    48001 gttcctgatc gacaatggta cgcagataat ccgggttggc ccgaatttgc ggttgctcat
    48061 tcttcaggcg atcgaacgtt ttctgcgccg cctcgtccat cagcttatac ggattggtct
    48121 ggtctgccgc ggttgccgca ctcagcggtg caatcaccag caaagcgacc atcattaaac
    48181 gtttaaacat gcgtcggttc tcctgaaatt atttcgttgt acccacaggt tcagtggttt
    48241 cattattacc tggcgcagca gctggcgcat cgccactatt cttattgtca tcgcctttac
    48301 taccgtaaag gaactgacca ataagatctt ccagcaccat cgcagacttg gtgtcctgaa
    48361 ttgtatcgcc atccttcagg atagcagtcc ccagttccgg gtcttcaaaa ccgacgttta
    48421 atgccagata ttgttccccc agcaggccgg aagtacgaat gctcagcgaa ctggtatctg
    48481 gaatgtggtt ataacgttgt tcaatttcca gcgttacgcg cggcagatag gttttcgggt
    48541 ccagcgtaat atccgccacc cggcccacaa caacaccacc aatgctgacc ggagagcgcg
    48601 ctttcaggcc gccaatgtta tcgaacgtcg cataaagcgt gtaggtcggt tcagtacgta
    48661 tggacgtcac gttcgccgcc ttcaggcaaa caaacagcgc cgccagtagt gctgctaata
    48721 aaaagatacc cacccaaatt tcattttttt tcgtttgcat gaactcaatt cccaaacatc
    48781 aatgcggtca gcacaaaatc cagccccaga acagccagag acgagtggac aacggtgcga
    48841 gtggttgccc ggctaatccc ggcagacgtc gggatggcgt cgtagccgtt aaacaacgaa
    48901 atccacgtca ccgtgatggc gaacaccacg ctcttaatca gacagttgac cagatccata
    48961 cgccagtcga cggcattttg cattgccgac cagaagaacc cgctatcaat gcctttccaa
    49021 ctgacgccga ccagagatcc gccccagatc cccacggcga cgaaaataac cgtcaacagt
    49081 ggtaatgaaa taaccccagc ccagaaacgg ggagatataa cccgacgcaa cggatccacc
    49141 gccatcatct ccatactgga gagttgctct gtagcgcgca tcaggccgat ttctgcggtt
    49201 agcgccgaac cagcacgccc ggcaaacaac aacgcggcaa ccaccggccc cagttcacgc
    49261 agtagcgata acgccaccag catacccaga ctggtttccg cactataagt ggtcagaacc
    49321 agataacctt gcagccccaa caccattccg atgaacacgc cagaaaccac aataatcagc
    49381 atcgacagga cgccgacatt atagagctgg cgcaccagca gcggcgcatg tttgcgaaat
    49441 tccggtttgc cgaccagcgc attgaataac attaacccgg cccgcccgaa cgttctcagg
    49501 gttttaatcc ctttatgtcc gagcgacgcc agcgcattta acagcatgag tggcttaact
    49561 ccctggtaaa agatcagcgt gataatcgcc ggcaggatag cggaacggaa caggcccgtc
    49621 agctatcccg tccagaaact gacgtacgcg cggatcagga ttcgcctgca acgcctgggc
    49681 actgccatga gcgacaattt ttttgtccgc caggatccag gcgtgatcgg caatacttaa
    49741 cacttccggc acatcgtgag aaaccaccac acaagtcacg cccagcgcgc tgttcagctc
    49801 agaaatcagc ttcaccagta cgcccatggt gatgggatct tgcccaacaa agggttcatc
    49861 aaacatgatg agatccggct ccagcgcaat cgcacgcgcc agcgctgcac gccgcgccat
    49921 cccaccggaa agttcagaag gcattagttt agccgctcca cgcagcccca cggcctccag
    49981 cttcatcatc accgtactat gcaacaatgc cgcgggaagt tgggtatgtt cgcgcagtgg
    50041 ataggcgacg ttgtcaaata cgttcatatc agtgaacaat gccccggact gaaataacat
    50101 gctcatccgt ttgcgcactg tatacaggcg cgaacgagac atcgccggaa tattctcacc
    50161 atcgaaaaga atctcaccat gatctggtgc gatttgcccg ccaatcagac ggagtagcgt
    50221 cgttttaccg atgcccgatg gccccatgat cgccgtgatc ttccctcgcg gcacggtcag
    50281 ggaaatatta tcgaagatgc agcgattgcc acgcgtaaaa ctgacatcgc gcatatcgac
    50341 taaattcgcc acagactgct ccataattca cccttcgtct tgcgttgatt ttctaagcat
    50401 ggcgctcaat ttaaccttga acccaacata tttacagaat attacccgcc gtggttagcg
    50461 aaagctggca tttgttttac tttttagccg cataaagtca aaattaagaa tccgttacgg
    50521 ctttctgaaa atcttcagcg gaccggcgag tatacctgaa gaaaggacgt tagatgcttt
    50581 tagctacggc actgttaatt gttggtttac ttttggtcgt ttacagtgcc gaccgcctgg
    50641 tttttgccgc gtctattctt tgccgaacct ttggcatccc gccgctgatc atcggcatga
    50701 cggtggtcag tattggtaca tcgttaccag aaatcatcgt ctcgcttgcc gcgtctctgc
    50761 acgaacaacg cgatttagcc gttggtacag ccctcggctc aaacattatc aatatattgc
    50821 tgatcctcgg tctggctgcg ctggttcgtc cttttaccgt ccattctgat gttctacgcc
    50881 gtgaattacc cttaatgttg ttggtcagcg tggtggccgg ttccgtactc tatgacggac
    50941 aacttagtcg aagcgatggt atctttctcc tctttctggc tgtgctatgg ctgctgttca
    51001 ttgttaaact tgcacgtcag gctgaacgtc aggggactga cagcctgacc agagagcagc
    51061 ttgcagagct gcctcgtgac ggcggattgc ccgtcgcgtt tttatggctc ggcattgcgc
    51121 ttatcatcat gccagtggcc acgcggatgg tggttgataa cgccacggtg ctggcgaatt
    51181 actttgccat cagcgagttg acgatgggtc tgacggcaat tgctatcgga accagcctgc
    51241 cggaactggc aaccgcaata gcgggggttc gcaaaggtga aaacgacatt gctgtcggaa
    51301 atatcattgg cgcaaacatt tttaatattg tcatcgtgtt gggtttaccc gcgctgataa
    51361 cgccaggaga gattgatcca ctggcgtaca gtcgtgacta cagcgtgatg ttgctggtga
    51421 gcattatttt tgcgttgctg tgctggcggc gctccccgca accgggccgt ggtgtagggg
    51481 tattattaac tggcggattt atcgtatggc tggcgatgtt gtactggtta tcgccaatac
    51541 tcgttgaata actggaaacg cattatgtcg cacgtagagt tacaaccggg ttttgacttt
    51601 cagcaagcag gtaaagaagt cctggcgatt gaacgtgaat gcctggcgga gcttgatcaa
    51661 tacatcaatc agaatttcac gcttgcctgt gaaaagatgt tctggtgtaa agggaaagtt
    51721 gtcgtcatgg ggatggggaa atcggggcat attgggcgca aaatggcggc aacgtttgcc
    51781 agcaccggta caccttcatt tttcgtccat cctggtgaag ccgcgcatgg tgatttaggc
    51841 atggtcaccc cacaggatgt ggtgattgct atctctaact caggtgaatc cagcgaaatc
    51901 acggccttaa ttccagtgct taagcgtctt cacgtaccgt taatctgcat caccggtcgc
    51961 ccgaagagca gcatggcgcg cgccgcagat gttcatctgt gtgttaaagt agcgaaagaa
    52021 gcctgtccgt tagggctggc accgaccagc agcaccaccg ccacgctggt tatgggcgat
    52081 gccctcgctg tcgcgctgtt aaaagcacgc ggctttactg ccgaagattt tgcgctctca
    52141 catccaggcg gcgcactggg tcgtaaactt ctgctgcgcg taaacgatat tatgcatacg
    52201 ggcgatgaga tcccgcatgt taagaaaacg gccagtctgc gtgacgcatt gctggaagtt
    52261 acccgcaaaa atcttggtat gactgtcatt tgcgatgaca atatgatgat tgaaggcatc
    52321 tttaccgacg gtgatttacg ccgtgtcttc gatatgggcg tggatgttcg tcagttaagt
    52381 attgccgatg ttatgacgcc ggggggaata cgcgtgcgcc ctggcattct ggccgttgag
    52441 gcactgaact taatgcagtc ccgccatatc acctccgtga tggttgccga tggcgaccat
    52501 ttactcggtg tgttacatat gcatgattta ctgcgtgcag gcgtagtgta aagattcaag
    52561 gataaacaac aatgagcaaa gcaggtgcgt cgcttgcgac ctgttacggc cctgtcagcg
    52621 ccgacgttat ggcaaaagca gagaacattc gtctgctgat cctcgacgtt gatggcgtac
    52681 tgtcagatgg cctgatttat atgggcaata acggcgaaga gctgaaagcg ttcaatgttc
    52741 gtgacggtta tggcattcgt tgtgcgctca cctctgatat tgaagtcgct atcattaccg
    52801 ggcgaaaggc taaactggta gaagatcgtt gtgccacatt ggggatcact cacttgtatc
    52861 aggggcagtc aaacaaactg atcgccttta gcgatctgct ggaaaaactg gcgattgccc
    52921 cggaaaatgt ggcttatgtc ggcgatgatc tcatcgactg gccggtaatg gaaaaagtgg
    52981 gtttaagcgt cgccgtggcc gatgcgcatc cactgttgat cccgcgcgcc gattacgtga
    53041 cgcgcattgc tggcggtcgt ggcgcagtgc gagaagtttg cgacttatta ctcctggcgc
    53101 agggcaaact ggatgaagcc aaagggcaat cgatatgagt aaagccagac gttgggttat
    53161 cattgtgcta tcactggcgg ttctggtgat gatcggcatt aatatggccg aaaaagacga
    53221 taccgcccag gtggtcgtca acaacaatga tcccacctat aaaagcgagc atacggacac
    53281 gctcgtctat aacccagaag gggcactaag ctatcgattg attgctcaac acgttgaata
    53341 ttattccgat caggccgttt cgtggtttac gcagccggta cttaccacgt ttgataagga
    53401 taaaatcccg acatggtccg taaaagcaga taaagccaag ctgaccaatg accggatgct
    53461 ctatttatat ggacacgttg aagtcaacgc acttgtgccg gactctcaac ttcgcagaat
    53521 cacgacggat aacgcgcaga tcaatctggt gacgcaggat gttacctctg aagacctcgt
    53581 cacgttatac ggaacaacat ttaactccag cggtctgaaa atgcgcggca acttacgcag
    53641 caagaacgcc gagctgattg aaaaggttag aacatcctat gaaattcaaa acaaacaaac
    53701 tcagccttaa tcttgtgctt gccagctcac ttctggccgc cagcattccg gcatttgccg
    53761 taaccggaga cactgatcag ccgatccaca ttgaatcgga ccagcaatct cttgatatgc
    53821 aaggcaacgt ggttaccttt accggtaatg tcatcgtcac ccagggcacc atcaaaatta
    53881 atgccgacaa agtggtcgtt acccgtccgg gcggcgaaca aggtaaagaa gtgattgacg
    53941 gctacggtaa accggcaacg ttctaccaga tgcaggacaa cggtaaaccc gttgaaggtc
    54001 acgcttccca gatgcactac gaactggcaa aagattttgt cgttctgacg ggtaatgctt
    54061 atctgcagca ggtcgatagc aacattaagg gcgataagat cacttacctg gtgaaagagc
    54121 agaaaatgca ggctttcagc gacaaaggta agcgcgtaac caccgttctg gtgccgtcgc
    54181 agctgcagga caaaaacaat aaaggccaga ccccggcaca gaagaagggt aattaattcg
    54241 ttatggcaac attaactgca aagaatcttg caaaagccta taaaggccgt cgcgtggtag
    54301 aagacgtcag cctgaccgtc aactccgggg aaattgtcgg tctgctgggg ccaaacggtg
    54361 ccggtaagac caccactttc tacatggttg ttggcattgt gccgcgcgat gcgggcaaca
    54421 tcattattga tgatgacgat atcagtctgc tgcctctgca tgcacgcgcg cgccgcggta
    54481 tcggctatct gccgcaggaa gcctccattt tccgtcgcct cagcgtttac gataacctga
    54541 tggcggtact gcaaattcgt gacgacttgt ctgctgaaca acgtgaagac cgcgcgaacg
    54601 agctgatgga agagtttcac attgagcacc tgcgtgacag catggggcag tcactctccg
    54661 ggggtgaacg tcgccgtgta gaaattgccc gcgcactggc tgcgaatccg aaatttattc
    54721 tgctcgacga accgtttgcc ggggttgacc cgatctcggt tatcgacatt aaacgcatca
    54781 ttgagcacct gcgcgacagc ggcctgggcg tgctgatcac tgaccacaac gtgcgtgaaa
    54841 cactggcggt ttgtgaacgc gcttatatcg tcagtcaggg gcatttgatc gcccacggca
    54901 cgcctacaga aatcttacaa gacgaacacg ttaagcgtgt ataccttggg gaagacttca
    54961 gactctgata gggtagaagt ttgcgacgtt ttagcaggag agtacgattc tgaacatgaa
    55021 gcaaggtttg caactcaggc ttagccaaca actggcgatg acgccacagc tccaacaggc
    55081 aattcgtctg ttgcagttgt cgacgctgga acttcagcag gagctacagc aggcgctgga
    55141 gagtaatccg ctgcttgagc aaatcgacac tcatgaagaa atcgacaccc gcgaaacgca
    55201 agacagtgaa acgctggaca ccgccgacgc gctcgaacaa aaagagatgc cggaagagct
    55261 gccgctcgat gccagttggg acaccattta caccgctggt acaccatccg gcaccagcgg
    55321 tgactacatt gacgatgagc tgccggtcta tcagggcgaa acgacgcaga ccttgcagga
    55381 ttacctgatg tggcaggtcg agctgacacc gttttccgac actgaccgcg ctattgctac
    55441 ctctatcgtc gatgccgttg atgacaccgg ttatctgact gtcccgctgg aagatattct
    55501 cgaaagtatg ggcgatgaag agatcgacat cgacgaggtt gaagccgtcc ttaagcggat
    55561 ccaacggttt gatccggtcg gtgtagcggc aaaagatctg cgtgactgcc tgctaatcca
    55621 actctcccaa ttcgataaaa ccacgccgtg gctggaagag gccagactga tcattagcga
    55681 tcatctcgat ctgttagcca atcacgactt ccgcacttta atgcgcgtca cgcgtctgaa
    55741 agaagatgtg ctgaaagaag ccgtcaatct gatccagtcg ctcgatccgc gccccgggca
    55801 gtcgatccag actggcgaac ctgagtatgt cattccagat gtgctggtgc gtaagcataa
    55861 cggtcactgg acggtagaac tcaacagtga cagcattccg cgtctgcaaa tcaaccagca
    55921 ctacgcctcg atgtgcaata acgcgcgcaa cgatggtgac agccagttta tccgcagcaa
    55981 tctgcaggat gccaaatggt tgatcaagag tctggaaagc cgtaacgata cgctactgcg
    56041 cgtgagtcgc tgtatcgttg aacagcagca agccttcttt gagcaaggtg aagaatatat
    56101 gaaaccgatg gtactggccg atatcgccca ggctgtcgaa atgcatgaat cgacgatatc
    56161 tcgcgtgacc acgcaaaaat acctgcatag tccacgaggc atttttgaac tgaagtattt
    56221 cttttccagt cacgtcaata ccgagggcgg cggcgaagct tcctccacgg cgattcgtgc
    56281 gctggtgaag aaattaatcg cggcggaaaa cccagcgaaa ccgttgagcg acagcaagtt
    56341 aacctctttg ctgtcggaac aaggtatcat ggtggcacgc cgcactgttg cgaagtaccg
    56401 agagtcttta tccattccgc cgtcaaacca gcgtaaacag ctcgtttgac ccaaccgata
    56461 aggaagacac tatgcagctc aacattaccg gaaataacgt cgagatcacc gaggcactgc
    56521 gcgaatttgt tacagccaaa tttgccaaac ttgagcaata ttttgaccga atcaaccagg
    56581 tctatgttgt tctgaaagtg gagaaagtca cccacacctc agatgcaaca ctgcatgtaa
    56641 acggtggtga aattcatgcc agcgcggaag gtcaggacat gtacgctgcc attgatggct
    56701 taattgataa gctggcacgc cagttgacca aacataaaga taaactgaaa caacactaat
    56761 tgtccgggca attagcatgt gcatggcggc ctgttgtgcg gcacaacggg ccatttgtac
    56821 ggttaatgct ccgagcctgt tccactgttt gagtgggcag gttcttaggt gaaattatga
    56881 caaataatga tacaactcta cagcttagca gtgttcttaa cagggaatgt acgcgaagcc
    56941 gcgtccactg tcagagcaaa aaacgcgccc tggaaatcat cagcgagctg gcggcgaaac
    57001 aacttagcct gccgcctcag gtggtttttg aagctatcct gacgcgggaa aaaatgggca
    57061 gtaccggtat cggcaatggt attgccattc cgcatggcaa actggaagaa gatactctgc
    57121 gtgccgttgg cgttttcgtt cagctcgaaa cgcctatagc tttcgatgcc atcgacaacc
    57181 aaccggtgga cttacttttt gccctgctgg tgccggcaga ccaaactaaa acgcacctgc
    57241 atactctgtc gctggtggcg aaacgtctgg cggacaaaac catctgccgc cgtttacgcg
    57301 cagcccagag cgatgaagag ctgtatcaaa tcattacgga taccgaaggt actccggatg
    57361 aagcgtagtt attcggtaat gtctctttta gacgttgtga ggagaaacag tacatggtac
    57421 tgatgatcgt cagcggacgt tcaggttcag gtaaatctgt cgccctgcgt gcgctggaag
    57481 atatgggttt ttactgcgtg gataaccttc ccgtagtgtt tttacccgat ctggctcgaa
    57541 ccctggccga tcgcgagatt tctgccgccg tcagcattga tgttcgtaat atgccggagt
    57601 caccagaaat attcgaacag gcgatgagta acctgcctga cgctttctca ccgcaactac
    57661 tgttcctgga tgccgaccgt aataccttaa ttcgtcgtta cagtgacacg cgccgactgc
    57721 atccgctttc cagcaaaaac ctgtcgctgg aaagtgctat cgacaaagaa agcgatctgc
    57781 tggagcctct gcgttcgcga gcggatctga ttgtcgatac ctcagaaatg tccgttcacg
    57841 agctggcaga aatgctgcgt acccgtctgc tgggtaaacg tgaacgtgaa ctgaccatgg
    57901 tctttgagtc tttcggcttc aaacacggta tccctatcga tgcagattac gtctttgacg
    57961 tgcgcttctt gccgaacccg cactgggatc cgaaactgcg tccaatgaca ggtcttgata
    58021 aacctgtcgc cgcgttcctc gaccgccaca cagaagtaca caattttatc taccagacgc
    58081 gaagctatct tgagctatgg ttacctatgc tggaaaccaa caaccgtagc tacttgacgg
    58141 tcgccattgg ttgtaccggc gggaagcacc gttcggtgta tattgcagag caactggcag
    58201 actacttccg ctcgcgcggt aaaaacgtcc agtcacgcca tcgtacgctg gaaaaacgta
    58261 aaccatgacc gtcaagcaaa ctgttgaaat cacaaacaag ctgggcatgc atgcccggcc
    58321 tgcaatgaag ctgtttgaat taatgcaggg tttcgacgca gaagtgctgt tacgtaatga
    58381 cgaaggtacc gaggccgaag ccaacagcgt tattgcactg ctgatgctgg attctgccaa
    58441 aggacggcag attgaagttg aagcgaccgg tccacaggaa gaggaagcac tggccgccgt
    58501 tatcgccctc tttaattctg gttttgatga agattaatct tcatcacttt gacattcaaa
    58561 tcccttcaag ctcccccgac aataagaaaa gcgcgtactg aaatcgttct caatcaacgt
    58621 catttgtaca ttttgtgcgc ttttcacttt cagaagaacc ttaagaaaac cttaagaagc
    58681 attgtttagg tttcgtttaa gttaatcgac catactggag atcgtcagaa aatatttcca
    58741 ggagatggca tgattcgctt atctgaacaa agtcccctgg gcaccggaag acatcggaaa
    58801 tgttatgcgc atccggaaga tgcccaacgc tgtatcaaga ttgtctacca tcgtggcgat
    58861 ggtggcgata aagagatccg ccgtgagtta aagtactacg cgcatcttgg tcgccggtta
    58921 aaagactgga gtggaatacc gcgctatcac ggtaccgttg agacggattg cggaaccggt
    58981 tatgtctacg atgtgattgc agattttgac ggcaaaccct ccattacgct gaccgaattt
    59041 gctgaacaat gccgttacga agaagacatt gcacaactgc gccagctact gaaacagctg
    59101 aagcgttatt tgcaggataa ccgtatcgtg acgatgtcgc tgaagccgca gaatatcctc
    59161 tgtcaccgca tcagtgagtc agaggttatc cccgtcgtct gcgacaacat tggtgaaagc
    59221 acattgattc cactggcgac ctggtcgaag tggtgttgtc tgcgtaagca agagcggtta
    59281 tggaaacgat ttatcgccca accggcgctg gctattgccc tgcagaaaga tctgcaacct
    59341 cgtgagagca aaacgctggc cctcacttcg cgcgaagctt aatccagctg gtgctgttgc
    59401 ataaacgact caccgcctaa ctgatacatc tgccgtaaaa tccacgcctg acggctacgc
    59461 acgtagcccg atggcgcgga gactttgaaa cgaagtggat taggtaatac agcagccagt
    59521 aatgcagctt ccgagcgggt aagtttgctc gcgggtttgt ggaaataacg ttgtgccgca
    59581 gcttcgacgc caaacacacc gtcgccaaat tcggcgatat tcaggtaaac cgtcaggata
    59641 cgctttttgc tccagacagt ttctatcccc agcgttaatc cagcttccag cccttttcga
    59701 acccagctac gcccatccca taaaaagaga tttttggctg tctgctgaga aatcgttgac
    59761 gcaccgcgaa tacggttttc attgcgctcg ttgtgggcca gggcttgctc aatggaggcg
    59821 acatcaaagc cccagtgctc aggaaacttc tgatcttctg cggcaatcac cgccagtccc
    59881 atccacggcg aaatttgatc catactgacc cagtcagaat gtgccacgta acgaaaattg
    59941 ccatgcagcc aggcgctgac ttgtcgctcg gccattaccg ctgagaaagg aacaggcgca
    60001 acgctaaaca acgcgatgcc cccgccccag aaaacggcga ggacaaccat taaccgcaga
    60061 agaaaacggc gaacgaaact aaacaccgtt aagcggcttt tactcattca gccagaacca
    60121 gcacgcggga aaccagctta tcaatgccgc tcgccgcttc tgcaatgttc tgcgccagca
    60181 tatatgccgg ggtggtgaca atcttattgt cttcatcaac cacgatatca tcgacaggac
    60241 atggcacatg ctccgcgccc atctcttcca gcacttctgc ggtatcgata tcagtaccga
    60301 tggtcaaacg cagcgggaaa tcgaaaattt tcggcagcat cgccggggca atacacataa
    60361 aaccaagcgg ttttccggcc tgatgcatcg cttgtgccag cgcctttaat tcacggtcaa
    60421 cggtgcattc gctaccaaga ctggcaaaat tgcttaaatt cttcgccgcg ccaaaacccc
    60481 ccggcacaat caacgcatcc agttcagcgg catcggcctg ggccagagga cggatttcac
    60541 cgcgcgttat tcgtgccgct tcaatcagca cattgcgcgt ttccgtcatc gcttcgcccg
    60601 ttaaatggtt gataacatca acctgctgct tatccggtgc aaagcagacc gcctgcgcac
    60661 cgctgcgtga aatagctaac aacgtcaaca ccgcttcatg aatttcagaa ccgtcataga
    60721 cgccgcatcc gctcagaatt acgccaattt tcttcattgt gatcatcctt tcgcaattga
    60781 ctgaaacaca ttaatttttt taataaaaat gctacgcatc acacatttaa ctgattcatg
    60841 taacaaatca tttaagtttt gctatcttaa ctgcgtgcgg cctgaaaaac agtgctgtgc
    60901 ccttgtaact catcataata atttacggcg cagccaagat ttccctggtg ttggcgcagt
    60961 attcgcgcac cccggtctag ccggggtcat tttttagtgg cttttgccac ccacgctttc
    61021 agtacttcta cgtcgtgacg ccactcttct ttcatctctt caatccattc accgacgtta
    61081 tcttcccagg cgggaaggtc aggagactga atttgctgac ccagctgttg cagatggcgt
    61141 aaccccactg accccgccgc acctttaatt ttatgtcctt cctcaacaat gcctttttta
    61201 tcctgcgccg tcagattcga ctccagcacg ctgacatagc ccggcatcat cttctcaaac
    61261 accgctaacc cgtcggtgat cagcttcggt cctacaagtt cgagatactg ttccagcatg
    61321 ggaatatcga gcaatgcttc tgatttactg ttctcttctg tcgtcaccgt actctcctca
    61381 tcatcctggg tatcccagaa tttcttgatc atcgcggtta gcgccggaac agaaagcggc
    61441 ttactcagca catcatccat tccagcattg aggtactctt gtttgtcttt cagcacgtta
    61501 gcggttaagg ccaccagcgg cggtaaatcc tcgcgcggat aacgcttcgt cagttcacga
    61561 gagatatcca gcccggtcat atctggcaac tgaatatcca gcaataccag gtcgtattcg
    61621 cccggtttaa acatctccag cgccgccttg ccggtcatgg cgacatcaac gctgttacct
    61681 aatttttcca gcacagaacg cgcgacaatc acgttcagtt caatgtcttc caccagcagc
    61741 acattcagcg ccggtaaagg catatcgtct tcatcaaacg catcatcgac ctcttcagcc
    61801 accgacggtg cgtggatcgt caacgtaaag gttgaacctt tgccctgttc gctggtaacc
    61861 gtaatatcgc cgcccatatt tttcgccaga cgacgagaaa cggccagacc aataccggtg
    61921 ccggtggcag gtttaccgcc atgactgtct ttcacctggt aatacatggc aaagatttta
    61981 tccagctcat cctgcggaat gccgatacca gagtcttcca cttcaaaatg cagcatatcg
    62041 ccttcatcgt agcgcacgcg cacggtaacc tggccttgct gggtgaattt gacggcgtta
    62101 ctgatgagat tccacaggat ctgccgtaaa cgcgtcccgt cggtaatgac ctgatgcggt
    62161 aatggcagcg taggctccag attaaagcgc aatccttttt gttgcgcctg caatgcggag
    62221 agattttcca gatcggcaag gaagctggtg aaatcaaccg gctgattatc aagctggacc
    62281 ttacgccgtt ccatcttatc catgtcgata atatcgttga agatattccc gagcgtgacg
    62341 gccgaaacat ggatggtttt gagatatttt tcctgctcgg cggtgagttc ggtatccagc
    62401 agaatgcggc tcaggccgac gataccattc agcggtgtac gcaattcgtg actgatggtg
    62461 gaaataaacg tcgttttgtc gcggctggcg cgttcaagcg catcctgata ccgcttacgc
    62521 tcggtaatgt cgcgaccaaa acccatcaaa ccgtgacgtt tacccacgcg gtcgtagtac
    62581 ggcactttac ggatttcaaa acaggctttg cgcccgtccg ggtaatccag ccactgttca
    62641 taggtcagtg acacattatg acggaacact ttttcatcgg tttcaatgac tttcgcagcg
    62701 gcttccggtg agtaaacatc agcaggtttc aggtgaacca gttgtttttc gctttttccg
    62761 gtcagcagct ccatcgcgcg gttacagccg gaaaactctt tatcttcgtt acgataaaaa
    62821 accaggtcgg gtgaagcgtc aaggaaggaa cgtaagaatg aggattgctg ctcgagctga
    62881 atttgtgtct cttcgcgctc tttgatttca attttcagtt ggccgaaggt ttcctgcagt
    62941 tctgcttccg ctttttcacg aacggcaatt tcctgattta gctgggcaat attatctttt
    63001 aactgaacgt tgaggctcaa atcgcgctcg cgcatctcct ccagtttttg caccagccgt
    63061 gacagacgtt gtcgtgactc ctccaattgc tcgacgacca ccgatagaaa gtagaccgcc
    63121 catggcgtaa tcagcaaacc aaagaagata gaacgaataa catcaatgct ttcgacctga
    63181 ccatgcagca ccatggttac cgccatttgt accacaatgg cgagtacaac gagggccaac
    63241 gccagcaaca ttgagaagcg caccagacct aacttcatca tcaggtcaac ataatactgc
    63301 gccagcagac gaatttgctt cattagggaa ttccttcacg acaacctgcc ccaataatac
    63361 ccaattctga cagttacgtt ataaattgtg tgagaaatgc ggaattcccc tcaacgacct
    63421 actccgttgg aggtagccac ggacgtccca gcgccgatcc ctgtacgcca tgttcattca
    63481 gatagcgatc cagctcgacc attcccgtcc atcgattttc gcaccacaga ggagcaagca
    63541 gcgtcgggcg acgggcgctg gcggaaatgc ggtggtagat cacttccggt ggcgtatggc
    63601 gaatcatttc tccggcagtg agcgtgtaat cctccagttc aataccgttc aaacgccccg
    63661 cttcccaggc tttcgccata atgctgcctt tcactatatg cagcggatgc agctttatgc
    63721 cgtccacgcc ggtttcaacc actcgctcca gcgtttgcaa acattcagcc tgcccttcgc
    63781 caggcaggcc aacaattaag tgagaacaca ccttcagccc acgctcacgc gccagctgcg
    63841 tcgtgcgctg atagcaggca aaatcatgac cgcgattgat gcgatgcagt gttttgtcgt
    63901 gggcggtttg tagccccagc tccagccaca cttcgtagcc ctggtcctta tattcgcaaa
    63961 gcagatccag caccgcatcc ggcacgcagt ccgggcgggt accaacacac aaaccgacaa
    64021 tattggcctg gctcaccgcc tgctgataca tcgaacgcag cacctgaact tccgcaaagg
    64081 tgctggtata cgcctgaaag taggccagat agcgtttagc gcggttaact aaattcgcct
    64141 ggtgcgccag ttgctcggca atggaacgat gctgctgcgc ttcatcggca aacgaggcaa
    64201 cattacagaa tgtgcagccg ccacgcccga tggtaccgtc acggttaggg cagctaaaac
    64261 cgccatgcag cgtcagctta tgcacctttt gcccataacg acgggtgaga tcaccaccaa
    64321 acatattgac taatttctgt aactgcataa tctgatagac cgcgccttga aaagaggcca
    64381 aagcctgcca tttttagctc aattcggcga tgacctggat caatcgtccc ggcctgcttt
    64441 tatcaactgc ataatcaatc aaaattaccg aaatttcatg cataatcaca taaatcactt
    64501 ttgcttatct tgtgtcagat ttttttatct ccttatggat tttaggcaaa aacagtagca
    64561 tgaaacgtca ttaccaatta aggcagtata aaatgctggt tttctcgtca gttcaaggca
    64621 ggataagggt taacacgcct ttatgacagt caggaattgg cagtttctct aacgactccc
    64681 cttttagcct taaagagaaa atccatttta atttcagcta attaataaag aattttgcgc
    64741 taaagcacat ttctgtacca ataagcttgc catttgacct gtatcagctt tcccgataag
    64801 ttggaaatcc gctggaagct ttctggatga gcagcctgct catcatattt atgcagtaat
    64861 tgagatcccc tcttcaccgt attaaccgat gcgaaaagga caacaagggg gcgaatgcga
    64921 ggcgcgcgta tgacacgcaa accccgtcgc cacgctcttt ctgtgcccgt gcgcagcggt
    64981 tcggaagtgg ggttcccgca gagcctgggg gaggttcacg atatgttgta cgataaatcc
    65041 cttgagaggg ataactgtgg tttcggcctg atcgcccaca tagaaggcga acctagccac
    65101 aaggtagtgc gtactgcaat acacgcactg gcccgcatgc agcaccgtgg cgcgattctc
    65161 gccgatggta aaaccggtga cggttgcggc ttgctgttac aaaaaccgga tcgctttttt
    65221 cgcatcgttg cgcaggagcg cggctggcgt ttagcaaaaa actacgccgt cgggatgctc
    65281 ttcctgaata atgatcctga actcgccgct gccgcacgcc gcatcgttga agaagagctg
    65341 caacgcgaaa cgttgtcgat tgtgggctgg cgtgatgtcc ccaccaacga aggcgtgctg
    65401 ggtgaaatcg ccctctcctc tctgccacgc attgagcaaa tttttgtgaa cgccccggca
    65461 ggctggcgtc cgcgcgatat ggagcgccgt ctgtttatcg cccgccgccg cattgaaaag
    65521 cgtctcgaag ccgacaaaga cttctacgtc tgtagcctgt cgaatctggt caatatttat
    65581 aaaggtctgt gtatgccggc ggatctgccg cgcttctacc tggacctcgc ggacctgcgt
    65641 ctggaatcgg ctatctgcct gtttcaccag cgcttttcca ctaacaccgt accgcgctgg
    65701 ccgctggcgc agccgttccg ctacctcgcg cataacggtg aaatcaacac catcaccggt
    65761 aaccgccaat gggcgcgtgc gcgtacctat aaattccaga cgccgctgat ccctgacctg
    65821 cacgacgccg cgccgttcgt caacgaaacc ggctctgact ccagttcgat ggataacatg
    65881 ctggaactcc tgctggcagg cgggatggat atcatccgtg ccatgcgtct gttagtgcca
    65941 ccagcctggc agaacaaccc ggatatggac ccggaactgc gtgccttctt tgactttaac
    66001 tccatgcata tggagccgtg ggatggtccg gcagggatcg tgatgtccga tggccgtttc
    66061 gccgcctgta acctcgaccg taacggtctg cgtccggcac gctacgtcat caccaaagat
    66121 aaactgatca cctgcgcctc tgaagtcggt atctgggatt atcagcctga cgaagtggtc
    66181 gaaaaaggcc gcgtagggcc aggcgaactg atggtgatcg acactcgcag tgggcgcatt
    66241 ctacactcgg cagaaaccga tgatgacctg aaaagccgcc atccctataa agagtggatg
    66301 gagaaaaacg tccgccgtct ggtaccgttt gaagatctgc ccgatgaaga agtgggtagc
    66361 cgcgaactgg acgacgacac gcttgccagc taccagaaac agtttaacta cagcgcggaa
    66421 gagctggatt cggtgatccg cgtactgggt gaaaacggtc aggaagcggt cggttcgatg
    66481 ggcgacgata ccccattcgc cgtgctctcc agccagccgc gcattattta cgactacttc
    66541 cgccagcagt ttgctcaggt aaccaacccg ccaatcgacc cgctgcgcga agcgcatgtt
    66601 atgtcgctcg ccaccagtat cggtcgtgaa atgaacgtct tctgtgaagc agaaggccag
    66661 gcgcaccgtt taagctttaa atcgccgatt ctgctctatt ccgatttcaa acagctcacg
    66721 acgatgaaag aggatcacta ccgcgcagat acgctggata tcacctttga cgtcactaaa
    66781 accacgctcg aagcgacggt gaaagagctg tgcgacaaag ccgaaaaaat ggtacgtagt
    66841 ggcaccgtgc tgctggtgct ctccgaccgg aatatcgcta aagatcgcct gccggttccg
    66901 gctccgatgg cggttggcgc gatccagacc cgtcttgtcg agcaaagcct gcgttgcgat
    66961 gccaacatca tcgtagaaac cgccagcgcc cgtgatccgc accacttcgc cgtgttgctg
    67021 ggtttcggcg cgacggctat ttatccatac cttgcctatg aaacgctggg ccgcctggta
    67081 gacacccacg cgattgccaa agattatcgt accgtgatgc tcaactatcg taacggcatc
    67141 aacaaaggct tgtacaaaat catgtccaaa atgggcatct ccaccatcgc ctcttaccgc
    67201 tgctcgaaac tgtttgaagc ggtcggtctg cacgatgatg tcgtgggcct gtgcttccag
    67261 ggggcggtta gccgcattgg gggggctagc tttgaagact tccagcagga tctgctgaat
    67321 ctgtcgaaac gagcctggct ggcgcgtaag cctatcagcc agggcggcct gctgaaatac
    67381 gtgcacggtg gcgaatacca cgcctacaat ccggacgtgg tgcgcacgct gcaacaggcg
    67441 gtacagagcg gcgagtatag cgactatcag gaatacgcga agctggttaa tgagcgtccg
    67501 gcaaccacgc tacgcgatct gctggcaatt acgccgggtg aaaacgcggt caacattgct
    67561 gatgttgaac cggcaagcga actgtttaaa cgcttcgata ccgccgcgat gtctatcggc
    67621 gcgttaagcc cggaagccca cgaggcgctg gcggaagcga tgaacagcat cggcggtaac
    67681 tcaaactccg gcgaaggcgg tgaagacccg gcgcgctatg gcactaacaa agtgtcgcgc
    67741 atcaagcagg tggcgtccgg tcgcttcggc gttactccgg cgtacctggt caatgccgac
    67801 gtcattcaga taaaagtcgc ccagggcgcg aagccgggtg aaggtggtca gttaccgggt
    67861 gataaagtca ctccttacat tgccaaactg cgctattcgg tgccgggcgt gacgctgatc
    67921 tccccgccgc cgcaccacga tatctactct attgaggatt tagcgcagct gattttcgac
    67981 ctcaagcagg ttaacccgaa agcgatgatc tccgtgaagc tggtttccga accgggcgta
    68041 ggcaccatcg cgactggcgt ggcaaaagct tatgccgatt taatcaccat cacaggctat
    68101 gacggcggca ccggcgcaag tccgctttca tcggtgaaat acgcaggctg tccgtgggag
    68161 ctggggcttg ttgaaaccca acaggcgctg gttgccaacg gcttgcgtca caagatccgt
    68221 ttgcaggtcg atggcggcct gaaaacgggt gtcgatatca tcaaagcagc gattcttggc
    68281 gcggaaagtt ttggcttcgg cactggcccg atggtagcgc ttggctgtaa atatctgcga
    68341 atttgccacc tgaacaactg cgccacgggt gtagcaactc aggatgacaa actgcgtaag
    68401 aatcactatc acggcctgcc gttcaaagtg actaattact ttgagtttat cgcccgtgaa
    68461 acccgcgagc tgatggcaca gctgggggta acgcgtctgg tggatctgat tggtcgcacc
    68521 gacctgctga aagagctgga cggtttcacc gccaaacagc agaaactggc actgtcgaag
    68581 ctgctggaga ctgctgagcc gcatcctggt aaagcgctct actgcaccga aaacaacccg
    68641 ccgtttgata acggcctgct gaacgcgcag ttgctgcaac aggcaaaacc gtttgtcgat
    68701 gaacgccaga gcaaaacctt ctggttcgat atccgcaaca ccgaccgttc tgtcggcgca
    68761 tcgctttcag gctatatcgc ccagacgcac ggcgatcagg ggctggctgc cgatcctatc
    68821 aaagcgtact tcaacggcac cgcaggccag agcttcggcg tgtggaacgc gggcggcgtg
    68881 gaactgtacc tgaccggtga tgccaacgac tatgtcggta aaggcatggc gggcggctta
    68941 atcgccattc gtcctccggt tggctcagcc ttccgcagcc atgaagcgag cattatcggc
    69001 aacacctgcc tgtatggcgc gaccggtggt cgtctgtatg ccgcaggccg cgcgggtgaa
    69061 cgtttcggcg tgcgtaactc cggtgctatc accgtggtag aaggcattgg cgacaacggc
    69121 tgtgaatata tgacgggtgg tatcgtctgt attctgggta aaaccggcgt taacttcggc
    69181 gcaggcatga ctggcggctt cgcttacgtt ctcgatgaaa gcggcgattt ccgcaaacgc
    69241 gttaacccgg aactggtcga ggtcttaagc gttgacgatc tggcgatcca tgaagagcat
    69301 ctgcgcggtc ttatcaccga gcatgttcag cataccggtt ctcagcgcgg tgaagagatt
    69361 ctggcgaact ggtcaacctt cgccactaaa tttgcgctgg ttaaaccgaa gtccagtgat
    69421 gtaaaagcac tgctgggtca ccgtagtcgt agcgcagcag agttgcgcgt gcaggcgcag
    69481 taaggggtag caacaatgag tcagaatgtt tatcaattta tcgacctgca gcgcgttgat
    69541 ccgcctaaga aaccgctgaa gatccgcaaa attgagtttg ttgaaattta cgagccgttt
    69601 tccgaaggcc aggccaaagc gcaggctgac cgctgcctgt cgtgcggcaa cccatactgc
    69661 gagtggaaat gcccggtaca caactacatc ccgaactggc tgaagctcgc caacgagggg
    69721 cgtatttttg aagcggcgga actgtcgcac cagaccaaca ccctgccgga agtatgcgga
    69781 cgtgtctgcc cgcaagaccg tctgtgcgaa ggttcctgca ccctgaacga tgagtttggc
    69841 gctgtgacca tcggcaacat tgagcgttat atcaacgata aagcgttcga aatgggctgg
    69901 cgtccggatc tgtccggcgt gaaacagacc ggtaaaaaag tggcgattat cggcgctggc
    69961 ccggcgggtc tggcgtgtgc ggatgttctg acgcgcaacg gcgtaaaagc ggtcgtcttc
    70021 gaccgtcacc cggaaattgg cggattgctg accttcggta ttccggcttt caagctggaa
    70081 aaagaggtaa tgacgcgccg ccgtgaaatc ttcaccggca tgggtattga attcaaactc
    70141 aataccgaag tgggccgcga cgtgcagctg gacgatctgc tgagtgatta cgatgccgtg
    70201 ttccttggcg tcgggactta tcagtcaatg cgcggcgggc tggaaaacga agacgccgat
    70261 ggcgtgtacg cagcgctgcc gttccttatc gccaacacca aacagttaat gggctttggt
    70321 gaaacccgcg acgaaccgtt cgtcagcatg gaaggcaaac gcgtggtggt ccttggcggt
    70381 ggcgacactg cgatggactg cgtgcgcacg tctgtacgcc agggggcaaa gcacgttacc
    70441 tgtgcctatc gtcgtgatga agagaacatg ccgggttccc gccgcgaagt gaaaaacgcg
    70501 cgggaagaag gcgtagagtt caaattcaac gtccagccgc tgggtataga agtgaatggc
    70561 aacggcaaag tcagcggcgt aaaaatggtg cgcacagaaa tgggcgaacc ggatgccaaa
    70621 ggccgtcgcc gcgcggagat tgtggcaggt tccgaacata tcgttccggc agatgcggtg
    70681 atcatggcgt ttggtttccg tccacacagc atggaatggc tggcaaaaca cagcgtcgag
    70741 ctggattcgc aagggcgcat tatcgccccg gaaggcagcg acaacgcttt ccagaccagc
    70801 aacccgaaaa tctttgctgg cggtgatatc gtccgaggtt ccgatctggt ggtgaccgct
    70861 attgccgaag gtcgtaaggc ggcagacggt attatgaact ggctggaagt ttaagctctg
    70921 tcccagcgcc gcctcctcag aaaagagtcg gcgctgaaat tctcctttct ttctggaaag
    70981 cgcctcgcat aatcttaaga aaaccaatgt aatacgctca tctggattgt taccggtaga
    71041 tgcccggcgt atagctgatt ccattattca acgcagcgcc atttaaaggg acagataatg
    71101 gaatcgctct cagagagaac cacagcaggc taccagcaaa tccacgacgg cattattcat
    71161 ctggtcgata gcgcccggac ggaaacggta cgtagcgtta acgcactgat gaccgcgaca
    71221 tactgggaaa ttggccgacg aattgtcgaa tttgaacaag gcggtgaggc cagggctgcg
    71281 tatggtgcgc agctaatcaa gcgactatca aaggatttaa gtctaaggta taagcgtggg
    71341 ttctctgcaa aaaacttacg ccaaatgagg cttttttacc tcttttttca acatgttgaa
    71401 tttcgccaga cagtgtctgg cgaattaaca ccattgggaa ttccgcagac accgtctgca
    71461 gaattcccat cggcaaagat ttggcagacg ctgtctgcca aatctttccc cctgccctgg
    71521 tccacctacg tccgtttact ttccgttaaa aaccctgatg cccgcagctt ttatgaaaaa
    71581 gagacgctcc gctgtggttg gtctgtccgc cagctagagc ggcaaattac gacccaattt
    71641 tatgagcgaa cactactgtc acatgacaaa tcagccatgc tgcaacaaca cgctcctgcc
    71701 gagatgcata ttcttccgca acaggcgata cgcgatccct ttgtgctcga atttctggag
    71761 ttgaaagatg aatactcaga atctgatttt gaggaggcgc tgatcaacca cctgatggat
    71821 ttcatgctgg aacttgggga tgattttgcc tttgttggtc ggcagcgaag gttacgcatt
    71881 gatgacaact ggtttcgggt cgatctgctg tttttccacc gccgtttacg ctgcctgcta
    71941 atcgtcgatc taaaagtggg caaattcagc tatagcgatg ccggacagat gaatatgtat
    72001 ctcaactacg ccaaagagca ctggacacta ccggatgaaa atccgcccat cggtctggtt
    72061 ctctgtgcag agaaaggagc cggcgaagcg cattatgcac tggctggttt gcctaacacc
    72121 gttctggcga gtgaatataa gatgcaacta cctgatgaaa aacgactcgc ggatgaactc
    72181 gttcgaacac aggcggtgtt agaggaaggc tatagacgcc gttaatttca gcggtttcga
    72241 attctccaga cactgtctgg agaatcttta aaacgaacta agttttaagc cattaaatca
    72301 gccttaacct tcaccacaac ctttttaatc tcaccaggtt cgccgacaac gcatcctggt
    72361 ttatgcggtt ctcccggcat aaacacggcg aacattcccg gttttaagat gatggcttgc
    72421 tcgttctcaa tggcgctgca aagctggtaa tcatcctcat ggtggaactc ttcacactga
    72481 cgcgcagtgc ctgccgtgcc aaacagaatc cgttcctcac cgtttaataa cagctggata
    72541 tcaatgtatt gctcgtgcag ctccgctttt ttctcaacgg gcgattgagt gttaaacgtc
    72601 atgacattca taaagatatt gtcgccctgt aattcgtaac gacccggtgc tttttcttgc
    72661 ggcctggcgg ctaaggctag tgttaacgcg tcctgtaacg caggatgcaa tccggcagac
    72721 ggtaacgact gtacttcacc cattatcata atttttctcc ctgggccaac aacgcagccc
    72781 caagtaaacc tgcatcgtgg cggtagtgcg ccgccagtaa atcaacatga aatgccgctg
    72841 gctcctgcgc cagatacgtt tccaccaacg ccagataccc ttctgccaga ccaacgctgc
    72901 caccgaccac tacgcactgg caatcagttg tggctttaat atcagcgatc agccttgcca
    72961 gcacatgtgc ggagctgtga atcagttgct gcgcctgctc atcgccctgc ccggcgcgtg
    73021 tgaaaatagt tctcgcatcc gcgcccgcca gttcaccctg cgctgccgct gcaataccgc
    73081 gaccagaagc aatcgcttcc acacaaccag tgcgtccaca gccgcagact ggaccatgag
    73141 gatcagccag cgtatgcccg atgtgccccg ccagaccgcc agggccggtg agcagtttgc
    73201 cgccgctcac tacaccgccg ccaacaccag tggaaacggt gataaacacc atctcggtta
    73261 tatcgccttc cagcgcctga tactccgccc acgctgcggc ctgcgcgtcg ttaatggcaa
    73321 tggtcggcaa atcggtaagt tgttccaaag ttttgactaa cggaaagtgt agcaatccac
    73381 cgagattatg cggattcagc gccagcaggc tgccgtcgcg aataattccg gttgaagcga
    73441 tagcaacccg ctgcgcatga acttgcaacg gagagactaa tgcggataag gcatcatgca
    73501 aggcttctgg tgtctggctg gctggcgtag gaagttcacg acgatcgcgg atctgcccat
    73561 cagcgccaat cagcgcggcg gcaagtttag taccgccgat atcaatcgcc agtgtggtca
    73621 tagcaccgcc tttttcatcg ctgtgttgta ccactgacaa atgtgttcaa gacgcgtaat
    73681 ggcagaaccg accgtcaccg cccacgcgcc gtggcgcatc gcatctgccg cctgagcagg
    73741 cgtgttgtaa cgcccttcgg caatcacccg acatccggcg tcgctcaacg ttttcaccag
    73801 cgccagatcc ggctcttctg gcgtttcagg cgtggtatag ccagaaagcg tagtgccaat
    73861 aatttcggct cccagctttt ggcatgccag gccgtcttcc ggcgttgagc agtcggtcat
    73921 cgccagtaaa ccgtgatggt gaatacgtgc cagcagcgtt tcaacaggca ccggacgcgg
    73981 tcggtcggtg ccgtcaatgg cgataatgtc cgcgcccgcc tgcgccagcg catcaacatc
    74041 ttcaatatag gccgtgatgc gtaccggaga atcctccaga tcgcgtttca caatcccgat
    74101 aattggcacg ctcaccaccg cacgcgtggc ttgcagattt gccacacctt caatgcgaat
    74161 ggcaaccgcg cccgcctgtt ctgccgctaa tgccatggcg gcgacgattt cgggtttatc
    74221 gagcgggctg tccggaaccg gctggcagga gacaatcagg ccaccgttag cagcgatttt
    74281 ttgatccagt tgtgcaagta acgacataca tcttccctta gcgaaaggcc cggtacagag
    74341 accgggcaac aggattaact tttggttttg actaaatcgt ttttggcgcg gccaaatggc
    74401 acggcaccgc taaatggttt gccgtcgata gcgtcatgag tacgcaatgc ttccgggcgc
    74461 agccaacgct gaacgcggga aggcatatcc agaccaatca gcaggatcac cacaaatgtc
    74521 agactgaacg agagcgatgc cagcgcagta cccagatcca gacgttgagc gatcaacgcg
    74581 ccgatgattg gggccagtgc accgcccaat gcgccgacgt tgtaggtaaa gcccaggccc
    74641 gctgcacgct ggtcggtatc gaaataaccg ccaatcagtt tcggtaagat cccggcgatc
    74701 ccttgtccaa gcatttgctg gaagaacagt aacagaccga gcacccagac gtttgcgccg
    74761 ccaatcgcaa ataccggaat aatcagcagc tgcgaggcca gcagactaca aacgtacgct
    74821 ttgcgggttc ccagccagtc accgaggaag ccaccgacgc agcaccccac cgccgcgcca
    74881 aagccactaa agaacagcac attggcgaca gtatgcgggt cataagccag atcggttttc
    74941 agatacgttg gcagcagcgc ctgaatcggc catgagtaga ggaaagcaaa caacacgacc
    75001 accatcagca ttacgcccgt tggccagcgt ttgcccgtac tctgcaccat aaagctgata
    75061 aagattgcgg cgcataacag cccgagtaca gcgacgattg cggcattttg caggttaccg
    75121 gcgaagcaga accacagcgc agtagccgcc gccagcgtca ttacgatatt ggcaatgcga
    75181 tgttcaccac ggtaaagaat gtctaccatc gtgcgtactg gtgctttacc cccgtgtttc
    75241 tctttccagt cttccgcttc cgggatgttt ttacgcagcc agagagcaaa gatgattggc
    75301 aaaatgccga taaagaacag cgctctccag ccccagaccg gaaccaccag actatagacc
    75361 tgagcggcaa cgacggcccc cacagagaag cctgaaatca aaaaaccact ggctttgtta
    75421 cgcagatgtt ttggccagct ttcaatgaca taggtggcgc tggaaccgta ttcacccgcc
    75481 atccccatgc cgatgaccag acgcgcgata aacatggtga tgtagcctgg cgcaaagccg
    75541 caggccagcg tcccggccga gaagagaacg atgctggtga ccattgccag acgacgcccg
    75601 tagcggtcac ccatagcgcc gagcatcagg ccgccgaacc agcgagagat aaaggctgcg
    75661 gagatcagac ttgccgcctg caccgtcgtc agcccgaatt caccctgtac ttcggtgagt
    75721 accagggcga ttaaaacgaa atcaaaaccg tcaagcagat atcccaacca ggcagcggaa
    75781 aatgcgcgcc attgtgcacg gttgagatgg cgataccacg ggatattctg ggttgtagta
    75841 ctcattgtga gtctcccgcg gtgggcgatg cccacacgct ttggtatgaa aattgtaggg
    75901 tacagatgcg tttatttccc ctcacccggt aggggcgagc gaggggaaaa caactcaccc
    75961 gcgctcttgc atcaactgct gggccagcgc cttcagttct ggcagatatt tttcatctac
    76021 cggtccaaac ggtttgcggc acagcggcac agaaacgaca tccatataat gcaggacagt
    76081 tttcaggccg cggaatacgc ccgttttgat cagtaaatca atgactttgt tgcactcagt
    76141 ttgcagtttc tgcgcggtct ggatatcgcc ttctttcagc gccttcacga tcccctgata
    76201 tcgccagccc atgatgttgt aagtactgcc gataccacca tcagcgcctg ccagcaagcc
    76261 ggaagcgaag atttcgtcgt aaccgttata gagcaccaga tcaggatgtt cacgacggat
    76321 ctgttccatc tgatagagat cgccagaagt ctgtttcagc gcaccgacgc caggcaacgt
    76381 aaccagggtg ttgatctgat ccagggtcag ttttacccca ctcagggctg gaatgttgta
    76441 caccaccatc ggcaaaccat ccgccgaatc aataattgcc cgatagtgat cgcagtgttc
    76501 ttcaaagctg aaaggatagt agaacggcgt gacggcggag acggcatcga agccataacg
    76561 tttagccgat gccgcaagtt gttggctttc ggcggtgctg acgcaaccga cgtgggcgat
    76621 gagtttaatc ttacctttcg cctcttcagc gacgatttcc agtacctgtt cacgctcgga
    76681 aaggctttgt acaaaggctt cgccggtaga accacccacg tataaaccgt cgatgccctg
    76741 ctgaatattg aactgaacca ggcgacgcag actcgcttta tccagtgctt gttgttgatc
    76801 aaaaggagtc aggagtgcgg ccattacgcc acgtaaattc gttgccataa atacctctga
    76861 agtgatgctt atctgataaa cgatatacct ttatacctgt tataccagat caattaagca
    76921 acaccccata cagaaagctt ataatgcgat ctgcttcact aaagtggcat tatttctttt
    76981 tgttggtggt ctgaccgaag gcgtgccagg tagcagagac gctgttgaga tgcgattgca
    77041 acgcacgatc ggcttcatcg ggatcgtggc ggcggatcgc gtcaacgatc gcaatatgct
    77101 gttgatagct aacgttgtta tgctcgtgca gagcctgatc agccacggtt ggacgtgcgg
    77161 cgatcagcca gtcgagcaag gcaacgtgga tcgccatgaa gattgggtta ccggggatct
    77221 ccgccagcac gcggtggaaa tcaacgtctg aacgaatgaa tgccgcgttg ttatccagcg
    77281 actgactgtt gatttccagt gcttttgcca gcaaatcgat ttgctcatcg gtggcgtgtt
    77341 cagccgcata gcgcaccagg ctggattcaa agaacagacg taattgttcg aaatgggcaa
    77401 tcccgccagg atgagaaagg aaatctttcg ccatgccgga aagctcgccg atgatggtgt
    77461 ccgcagaagg acgtgagacg cgagcgcgtt cgccgttgtt tatctgcacc agacctttgc
    77521 gttttaacgc tgccagcgct tcgcgcaccg aaggacgccc gacgttaaag aacgccatca
    77581 gttcgcgttc agacggtaat tgttcacctt cgccaaattc acgacggcgg atcatctgtt
    77641 ccagctcttc ttccaccatt tcggagagtt ttttacgcgc cagcgggcgg ctgcgtaagt
    77701 tgcggccaat tgcaggtgaa gaatcttcgg tttgcgaatc aaatgcgttc ataaggccca
    77761 ttctgtaagg tcagtgtgat taacatcatc agtgacatcc tatcacagga ttgaaagtag
    77821 ggggaaatgg cggggttttc tctttgtgcc tcatcattac tataattaac tgaataatta
    77881 actat
//
LOCUS       DAPHCV010000005        77390 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.005, whole genome shotgun sequence.
ACCESSION   DAPHCV010000005 DAPHCV010000000
VERSION     DAPHCV010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 77390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.005"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            198..692
                     /gene="napF"
                     /locus_tag="RH033_000317"
     CDS             198..692
                     /gene="napF"
                     /locus_tag="RH033_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HDV2989560.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDVCI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            682..945
                     /gene="napD"
                     /locus_tag="RH033_000318"
     CDS             682..945
                     /gene="napD"
                     /locus_tag="RH033_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HDV2989561.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFTGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            942..3428
                     /gene="napA"
                     /locus_tag="RH033_000319"
     CDS             942..3428
                     /gene="napA"
                     /locus_tag="RH033_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HDV2989562.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKYPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3435..4130
                     /gene="napG"
                     /locus_tag="RH033_000320"
     CDS             3435..4130
                     /gene="napG"
                     /locus_tag="RH033_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HDV2989563.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4117..4980
                     /gene="napH"
                     /locus_tag="RH033_000321"
     CDS             4117..4980
                     /gene="napH"
                     /locus_tag="RH033_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HDV2989564.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            4977..5426
                     /gene="napB"
                     /locus_tag="RH033_000322"
     CDS             4977..5426
                     /gene="napB"
                     /locus_tag="RH033_000322"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HDV2989565.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5436..6038
                     /gene="napC"
                     /locus_tag="RH033_000323"
     CDS             5436..6038
                     /gene="napC"
                     /locus_tag="RH033_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HDV2989566.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6057..6674
                     /gene="ccmA"
                     /locus_tag="RH033_000324"
     CDS             6057..6674
                     /gene="ccmA"
                     /locus_tag="RH033_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HDV2989567.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGDVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTGAA"
     gene            6671..7333
                     /gene="ccmB"
                     /locus_tag="RH033_000325"
     CDS             6671..7333
                     /gene="ccmB"
                     /locus_tag="RH033_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HDV2989568.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAAVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7375..8112
                     /gene="ccmC"
                     /locus_tag="RH033_000326"
     CDS             7375..8112
                     /gene="ccmC"
                     /locus_tag="RH033_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HDV2989569.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8109..8318
                     /gene="ccmD"
                     /locus_tag="RH033_000327"
     CDS             8109..8318
                     /gene="ccmD"
                     /locus_tag="RH033_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HDV2989570.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8315..8794
                     /gene="ccmE"
                     /locus_tag="RH033_000328"
     CDS             8315..8794
                     /gene="ccmE"
                     /locus_tag="RH033_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HDV2989571.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8791..10734
                     /gene="ccmF"
                     /locus_tag="RH033_000329"
     CDS             8791..10734
                     /gene="ccmF"
                     /locus_tag="RH033_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HDV2989572.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVNPQKTAPEAV"
     gene            10731..11288
                     /gene="dsbE"
                     /locus_tag="RH033_000330"
     CDS             10731..11288
                     /gene="dsbE"
                     /locus_tag="RH033_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HDV2989573.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            11285..12337
                     /gene="ccmH"
                     /locus_tag="RH033_000331"
     CDS             11285..12337
                     /gene="ccmH"
                     /locus_tag="RH033_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HDV2989574.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMSKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(12372..13019)
                     /gene="narP"
                     /locus_tag="RH033_000332"
     CDS             complement(12372..13019)
                     /gene="narP"
                     /locus_tag="RH033_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HDV2989575.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            13339..14112
                     /locus_tag="RH033_000333"
                     /pseudo
     CDS             13339..14112
                     /locus_tag="RH033_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(14238..14314)
                     /locus_tag="RH033_000334"
     tRNA            complement(14238..14314)
                     /locus_tag="RH033_000334"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14278..14280),aa:Pro,seq:ggg)
     gene            complement(14389..16149)
                     /gene="pbgA"
                     /locus_tag="RH033_000335"
     CDS             complement(14389..16149)
                     /gene="pbgA"
                     /locus_tag="RH033_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HDV2989576.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            complement(16169..16396)
                     /locus_tag="RH033_000336"
     CDS             complement(16169..16396)
                     /locus_tag="RH033_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HDV2989577.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            16578..17585
                     /gene="yejK"
                     /locus_tag="RH033_000337"
     CDS             16578..17585
                     /gene="yejK"
                     /locus_tag="RH033_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HDV2989578.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            complement(17724..18008)
                     /gene="rplY"
                     /locus_tag="RH033_000338"
     CDS             complement(17724..18008)
                     /gene="rplY"
                     /locus_tag="RH033_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HDV2989579.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAVELDH
                     DKVMNMQVKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLLHIDFVRA"
     gene            complement(18133..19893)
                     /locus_tag="RH033_000339"
     CDS             complement(18133..19893)
                     /locus_tag="RH033_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDV2989580.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAATRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGSERDVLIDDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            20043..20738
                     /gene="rsuA"
                     /locus_tag="RH033_000340"
     CDS             20043..20738
                     /gene="rsuA"
                     /locus_tag="RH033_000340"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HDV2989581.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLVQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            20766..21956
                     /gene="bcr"
                     /locus_tag="RH033_000341"
     CDS             20766..21956
                     /gene="bcr"
                     /locus_tag="RH033_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HDV2989582.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNILFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            22290..22634
                     /locus_tag="RH033_000342"
     CDS             22290..22634
                     /locus_tag="RH033_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2989583.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(22638..24227)
                     /gene="yejF"
                     /locus_tag="RH033_000343"
     CDS             complement(22638..24227)
                     /gene="yejF"
                     /locus_tag="RH033_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HDV2989584.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIVFDGKPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(24229..25254)
                     /locus_tag="RH033_000344"
     CDS             complement(24229..25254)
                     /locus_tag="RH033_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2989585.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            complement(25254..26348)
                     /locus_tag="RH033_000345"
     CDS             complement(25254..26348)
                     /locus_tag="RH033_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HDV2989586.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNTGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            complement(26349..28163)
                     /locus_tag="RH033_000346"
     CDS             complement(26349..28163)
                     /locus_tag="RH033_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV2989587.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAAKNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNVTTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            complement(28245..29801)
                     /locus_tag="RH033_000347"
     CDS             complement(28245..29801)
                     /locus_tag="RH033_000347"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HDV2989588.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLKELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTFVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVVYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAEAQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPAHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEREATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            complement(29982..30548)
                     /gene="mepS"
                     /locus_tag="RH033_000348"
     CDS             complement(29982..30548)
                     /gene="mepS"
                     /locus_tag="RH033_000348"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HDV2989589.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            complement(30959..31672)
                     /gene="lpxT"
                     /locus_tag="RH033_000349"
     CDS             complement(30959..31672)
                     /gene="lpxT"
                     /locus_tag="RH033_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HDV2989590.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHYQNK"
     gene            complement(31711..32697)
                     /gene="yeiR"
                     /locus_tag="RH033_000350"
     CDS             complement(31711..32697)
                     /gene="yeiR"
                     /locus_tag="RH033_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HDV2989591.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKIDGHLLDLPRRNLAELPASAAHSHQHGVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVMRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            complement(32815..34281)
                     /locus_tag="RH033_000351"
     CDS             complement(32815..34281)
                     /locus_tag="RH033_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDV2989592.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQNPEEPHS
                     APGILVEALKRRRERGLAPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLARE
                     TDWSLLALGVAGWMRYVSGVDDAGNTIDVRDPLSDKIRDLVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIAQFGAHQALLNTLKI"
     gene            complement(34504..35076)
                     /gene="yeiP"
                     /locus_tag="RH033_000352"
     CDS             complement(34504..35076)
                     /gene="yeiP"
                     /locus_tag="RH033_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HDV2989593.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            35231..35485
                     /locus_tag="RH033_000353"
     CDS             35231..35485
                     /locus_tag="RH033_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDV2989594.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            complement(35482..36663)
                     /gene="setB"
                     /locus_tag="RH033_000354"
     CDS             complement(35482..36663)
                     /gene="setB"
                     /locus_tag="RH033_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HDV2989595.1"
                     /translation="MHNSPAVTSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLDTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            37030..38160
                     /gene="fruB"
                     /locus_tag="RH033_000355"
     CDS             37030..38160
                     /gene="fruB"
                     /locus_tag="RH033_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HDV2989596.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMDEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            38160..39098
                     /gene="fruK"
                     /locus_tag="RH033_000356"
     CDS             38160..39098
                     /gene="fruK"
                     /locus_tag="RH033_000356"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDV2989597.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            39115..40806
                     /gene="fruA"
                     /locus_tag="RH033_000357"
     CDS             39115..40806
                     /gene="fruA"
                     /locus_tag="RH033_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HDV2989598.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAAAAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     SKKEGAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            41230..42171
                     /locus_tag="RH033_000358"
     CDS             41230..42171
                     /locus_tag="RH033_000358"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HDV2989599.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGITLSGREDVAKVAAWFHQHGLN
                     RMVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            42159..43097
                     /gene="psuG"
                     /locus_tag="RH033_000359"
     CDS             42159..43097
                     /gene="psuG"
                     /locus_tag="RH033_000359"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HDV2989600.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPTTIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEHTINAAIDQAVAEAEAQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            43191..44441
                     /locus_tag="RH033_000360"
     CDS             43191..44441
                     /locus_tag="RH033_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDV2989601.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(44509..45207)
                     /gene="yeiL"
                     /locus_tag="RH033_000361"
     CDS             complement(44509..45207)
                     /gene="yeiL"
                     /locus_tag="RH033_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HDV2989602.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAID
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            45337..46278
                     /gene="rihB"
                     /locus_tag="RH033_000362"
     CDS             45337..46278
                     /gene="rihB"
                     /locus_tag="RH033_000362"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HDV2989603.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            46378..47628
                     /locus_tag="RH033_000363"
     CDS             46378..47628
                     /locus_tag="RH033_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDV2989604.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFINRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(47684..48772)
                     /locus_tag="RH033_000364"
     CDS             complement(47684..48772)
                     /locus_tag="RH033_000364"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDV2989605.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLDQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(48776..49633)
                     /gene="nfo"
                     /locus_tag="RH033_000365"
     CDS             complement(48776..49633)
                     /gene="nfo"
                     /locus_tag="RH033_000365"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDV2989606.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVAEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTSAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(49707..50756)
                     /locus_tag="RH033_000366"
     CDS             complement(49707..50756)
                     /locus_tag="RH033_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HDV2989607.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            50855..51736
                     /locus_tag="RH033_000367"
     CDS             50855..51736
                     /locus_tag="RH033_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2989608.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            51941..53410
                     /gene="lysP"
                     /locus_tag="RH033_000368"
     CDS             51941..53410
                     /gene="lysP"
                     /locus_tag="RH033_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HDV2989609.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            53703..55683
                     /gene="cirA"
                     /locus_tag="RH033_000369"
                     /pseudo
     CDS             53703..55683
                     /gene="cirA"
                     /locus_tag="RH033_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311074.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(55715..56551)
                     /gene="yeiG"
                     /locus_tag="RH033_000370"
     CDS             complement(55715..56551)
                     /gene="yeiG"
                     /locus_tag="RH033_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDV2989610.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            56809..57477
                     /gene="folE"
                     /locus_tag="RH033_000371"
     CDS             56809..57477
                     /gene="folE"
                     /locus_tag="RH033_000371"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDV2989611.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            57494..58651
                     /locus_tag="RH033_000372"
     CDS             57494..58651
                     /locus_tag="RH033_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HDV2989612.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAIGNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMQFDR
                     LELLAFVIPVWLANIFFSVIWLRYFRQGPVEWLWRQLTLRAAGPTISKTSR"
     gene            58793..59833
                     /gene="galS"
                     /locus_tag="RH033_000373"
     CDS             58793..59833
                     /gene="galS"
                     /locus_tag="RH033_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HDV2989613.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            60113..61111
                     /gene="mglB"
                     /locus_tag="RH033_000374"
     CDS             60113..61111
                     /gene="mglB"
                     /locus_tag="RH033_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HDV2989614.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            61172..62692
                     /gene="mglA"
                     /locus_tag="RH033_000375"
     CDS             61172..62692
                     /gene="mglA"
                     /locus_tag="RH033_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HDV2989615.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            62708..63718
                     /gene="mglC"
                     /locus_tag="RH033_000376"
     CDS             62708..63718
                     /gene="mglC"
                     /locus_tag="RH033_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HDV2989616.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(63977..65212)
                     /gene="preA"
                     /locus_tag="RH033_000377"
     CDS             complement(63977..65212)
                     /gene="preA"
                     /locus_tag="RH033_000377"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HDV2989617.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEDNLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQDMVGLANNNIVPAEDLDRSYI
                     VYPHINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IDLGEVKFKKGEKEHPVTL"
     gene            complement(65206..66444)
                     /gene="preT"
                     /locus_tag="RH033_000378"
     CDS             complement(65206..66444)
                     /gene="preT"
                     /locus_tag="RH033_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HDV2989618.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQAG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNKEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTINTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAGAIHHYLEGACSC"
     gene            complement(66763..67002)
                     /locus_tag="RH033_000379"
     CDS             complement(66763..67002)
                     /locus_tag="RH033_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HDV2989619.1"
                     /translation="MDVQQFFVVAVFFLIPVFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(67005..67724)
                     /gene="sanA"
                     /locus_tag="RH033_000380"
     CDS             complement(67005..67724)
                     /gene="sanA"
                     /locus_tag="RH033_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HDV2989620.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(67874..68758)
                     /gene="cdd"
                     /locus_tag="RH033_000381"
     CDS             complement(67874..68758)
                     /gene="cdd"
                     /locus_tag="RH033_000381"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDV2989621.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            complement(68888..69583)
                     /locus_tag="RH033_000382"
     CDS             complement(68888..69583)
                     /locus_tag="RH033_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HDV2989622.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(69580..69978)
                     /locus_tag="RH033_000383"
     CDS             complement(69580..69978)
                     /locus_tag="RH033_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDV2989623.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            complement(70109..71017)
                     /locus_tag="RH033_000384"
     CDS             complement(70109..71017)
                     /locus_tag="RH033_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2989624.1"
                     /translation="MANWAQKLKLHHLQMLVALGEQGNLTHVARMMNITQPALSKWLS
                     QLEDEIGLTLFERHSKGLRPTEGGKLLLQHAQRLINDMERSQHELARFKQGGLVGSLK
                     IGCSPVATDCVSQAILHLLKEMPTLHLNIEEKVMTPLLQDLLAGSVDVVVGRIGGRAL
                     QLPLNYRVLYTEPVCFVARPEHPLAKYATLSWNDLANWRWIVWPTGTPIRISIDNALV
                     DHGVMLPENTIESASMNVSTNLLQSSDMISILSLRLAQRYASQGQLVILNLPKIEQKG
                     SVGVFWRNNETPSYALSQFLHFLQQN"
     gene            71144..72502
                     /locus_tag="RH033_000385"
     CDS             71144..72502
                     /locus_tag="RH033_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid/H+ symport family MFS transporter"
                     /protein_id="HDV2989625.1"
                     /translation="MTQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFI
                     APDIRTHWQLTAGDLAPLFGAGLLGLTAGALLCGPLSDRFGRKRVIELCVFLFGALSL
                     ASAFSPDLQTLVFLRFLTGLGLGGAMPNTITMTSEYLPARRRGALVTLMFCGFTLGSA
                     FGGIVSAQLVPVIGWHGILVLGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTV
                     SAIIRERYVDTHFYLIESASVTKGSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSW
                     MPTLLNHRGIDLQHASWVTAAFQIGGTLGALALGVLMDKFNPFRVLALSYAIGAVCIV
                     MIGLSQDGLWLMALAIFGTGIGISGSQVGLNALTATLYPTQSRATGVSWSNAVGRCGA
                     IVGSLSGGVMMAMNFSFDTLFFIIAVPAAISAVMLTQLITVVRQSTSVPDSLPHAGVV
                     NE"
     gene            72514..73542
                     /gene="gtdA"
                     /locus_tag="RH033_000386"
     CDS             72514..73542
                     /gene="gtdA"
                     /locus_tag="RH033_000386"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HDV2989626.1"
                     /translation="MTDNNQNSREQFYQHISGQNLTPLWESLHHLVPKTPNANCAPAY
                     WNYQEIRPLLLESGSLIGAKEAVRRVLVLENPALRGQSSITATLYAGLQLIMPGEVAP
                     SHRHNQSALRFIVEGKGAFTAVDGERTSMNEGDFILTPQWRWHDHGNPGDEPVIWLDG
                     LDLPLVNTLGCGFAEDYPEEQQPVRRKEGDYLPRYAANMLPLRHQTGNSSPIFNYRYD
                     RSREVLHDLTRLGDADEWDGYKMRYVNPVTGGYPMPSMGAFLQLLPKGFASRVARTTD
                     STIYHVVEGSGQVTIGNETFSFSAKDIFVVPTWHGVSFQTTQDTVLFSFSDRPVQEAL
                     GLFREARY"
     gene            73558..74259
                     /locus_tag="RH033_000387"
     CDS             73558..74259
                     /locus_tag="RH033_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDV2989627.1"
                     /translation="MTQYVFEPQAPVTVPVVGSDEQFPVRRVYCVGRNYAAHAREMGF
                     DPDREPPFFFCKPADAIVPVAAGETLALPYPAQTDNYHYEIELVVAIGKKGSDIPLEN
                     AHEYVWGYATGLDMTRRDRQMEMRQMGRPWEIGKAFDLSAPIAPLHKASETGGIDNAP
                     IWLQVNGEDHQRSDIRHLIWSVNETISYLSGFFELQPGDLIFTGTPEGVGSVVKGDVI
                     TGNVEGLTPIAVKIV"
     gene            74267..74911
                     /gene="maiA"
                     /locus_tag="RH033_000388"
     CDS             74267..74911
                     /gene="maiA"
                     /locus_tag="RH033_000388"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HDV2989628.1"
                     /translation="MKLYSFFNSSASYRVRIALALKGIDYQTEGVNIRIGQQNELAYR
                     RMNPVGLVPTLLTDEGESLGQSLAIIDWLESHYPQVPLMPQEEPARNKVLEIVYAIAC
                     DIHPLNNLRVLRYLTEELNVSEEDKKRWYAHWIQQGLSAVEQLLRQSQSGQFCVGETP
                     TLADCCLVPQWANALRMNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA"
     gene            74926..76119
                     /locus_tag="RH033_000389"
     CDS             74926..76119
                     /locus_tag="RH033_000389"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HDV2989629.1"
                     /translation="MTKVTRAVIVGGGIGCAATALSLARQGIKVVLLEKAHEIGEIGA
                     GIQLGPNAFSALDSLGVGEIARQRAVFTDHITMMDAVNAEEVVRIETGQAFRDHFGGP
                     YAVIHRVDIHASVWEAVLTHPDVEYHTSTNVVDIRETEDDVTVFDDRGNSWIGDILIG
                     CDGVKSVVRQSLLGDAPRVTGHVVYRAVIDCADMPEDLRINAPVLWAGPCCHLVHYPL
                     RGGKQYNLVVTFHSRQQEEWGVKDGSKEEVLSYFEGIHPRPRQMLDKPTSWRRWSTAD
                     REPVAKWGTKRITLVGDAAHPVAQYMAQGACMALEDAVTLGKALAQCEGDAAQAFALY
                     ESVRIPRTARIVWSTREMGRVYHAAGVERQVRNLLWKGKSQAEFYRGMEWLYGWKEDN
                     CLQPR"
     gene            76181..77131
                     /gene="dusC"
                     /locus_tag="RH033_000390"
     CDS             76181..77131
                     /gene="dusC"
                     /locus_tag="RH033_000390"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HDV2989630.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPAGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMTISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIDKLR"
BASE COUNT        18487 a        19312 c        20783 g        18808 t
ORIGIN      
        1 tttgaaatgt gagcaaaagc ccgtttttgc cacgcttccc gccactcttt tgatcctgct
       61 acaggtttta ccccgatcgg ggtatgcatc tttgacacat cctttaatat cttagcggct
      121 ataaaaatgg cttattaatt atgcggttta tttggtcgct ctcaattttc agagcgcgtt
      181 aatgatggaa ggtcaatgtg aagattgatg catcccgtcg gggcatactc actggtcgct
      241 ggcgcaaagc cagtaacggt atccgtccgc cctggtccgg tgatgaatct cattttctga
      301 cccattgcac ccgctgtgat gtctgtatta atgcttgtga aaacaacatt ctgcaacgcg
      361 gcgcgggtgg ctatccgagc gttaatttca aaaataacga gtgcagcttc tgttacgcct
      421 gcgcgcaggc ttgccccgaa tcgctatttt ctccgcgcca caccagggcc tgggatttgc
      481 agttcaccat tggggacgcg tgcctggcgt atcagtcagt tgaatgccgc cgctgtcagg
      541 atagctgtga gccaatggca attatctttc gcccgacgct ctccgggatc taccagccgc
      601 aacttaatag ccaactttgc aacggatgtg gagcatgtgc agccagctgt ccggtatcag
      661 ccataactgc ggagtatctc catgcacact aactggcaag tttgcagcct ggtcgtgcag
      721 gccaaaagcg aacgaatttc agacatcagc acccaactga acgcctttac cggctgtgaa
      781 gttgctgtca gcgacgcgcc gagcggtcag ttgattgtgg tggtagaagc agaagacagc
      841 gaaacgctga tccaaaccat tgagtcagta cgcaacgtag agggcgtgct ggcggtgtcg
      901 ctggtttatc accagcagga agagcaaggt gaggaaacac catgaaactc agtcgtcgta
      961 gctttatgaa agctaacgcc gttgcggccg ctgcggcggc tgccggtctc agcgtgccgg
     1021 gcgttgcccg cgccgttgtt ggtcagcagg aagccattaa atgggataaa gcgccgtgcc
     1081 gtttctgcgg tacgggttgc ggcgttctgg tcggaacgca gcaggggcgt gtggtggcct
     1141 gtcagggcga cccggacgca ccggttaacc gtggcctgaa ctgcattaag ggctatttcc
     1201 tgcccaaaat catgtacggt aaagaccgtt tgacgcagcc gctgctgcgt atgaaaaacg
     1261 gtaaatatga caaagaaggc gaatttaccc caatcacctg ggatcaggcc ttcgatgtga
     1321 tggaagagaa atttaagact gccctgaaag aaaaagggcc ggaatcgatc ggtatgttcg
     1381 gttctggtca gtggactatc tgggaaggtt atgccgcgtc caagctgttt aaagcgggct
     1441 tccgttcgaa caacatcgac ccgaacgcgc gtcactgtat ggcgtcggca gtagttggct
     1501 ttatgcgtac ctttggtatg gatgagccga tgggctgcta cgacgacatc gagcaggctg
     1561 acgcgtttgt gctgtggggc gcaaacatgg cggagatgca cccgatcctc tggtcgcgca
     1621 tcactaaccg tcgtctctct aaccagaacg ttaccgtggc ggtgctttct acctaccagc
     1681 atcgtagctt cgagctggcg gataacggca tcatctttac gccgcaatct gacctggtga
     1741 tcctgaacta tatcgccaac tatatcattc aaaacaatgc gataaatcag gacttcttca
     1801 gcaaacacgt taacctgcgc aaaggggcga cggacatcgg ctacggttta cgtccgaccc
     1861 atccgctgga aaaagcagcg aagaatccgg gttctgacgc ctccgaaccg atgagctttg
     1921 aagattacaa agccttcgtt gctgaatata cgctggaaaa aaccgccgaa atgaccggcg
     1981 tgccgaaaga ccagttagaa caactggcgc agctgtatgc cgatccgaac aagaaagtca
     2041 tctcctactg gacgatgggc ttcaaccagc atacccgtgg tgtgtgggcc aacaacctgg
     2101 tctacaacct gcacctgctg accggcaaaa tttcccagcc gggttgcggt ccgttctccc
     2161 tgaccgggca gccttccgcg tgtggtactg cgcgtgaagt gggcaccttt gctcaccgtc
     2221 tgcctgcgga catggtggtg actaacgaga aacaccgtga tatctgcgag aagaagtgga
     2281 atatcccgag cggcaccatt ccggcgaaaa tcggtctgca cgcggtggca caagaccgtg
     2341 cgctgaaaga cggcaagctg aatgtttact ggaccatgtg taccaacaac atgcaggccg
     2401 ggccgaacat taatgaagag cgtatgccgg gctggcgcga tccgcgcaac tttatcatcg
     2461 tttccgatcc gtatccgaca gtcagtgcgc tggccgccga cttgatcctg ccgaccgcaa
     2521 tgtgggtaga gaaagagggc gcttacggta acgccgaacg ccgtactcag ttctggcgtc
     2581 agcaggtaca ggcaccgggc gaagcgaaat cggatctctg gcagttagtc cagttctccc
     2641 gccgcttcaa aacagaagaa gtatggccgg aagagctgct ggcgaagaaa ccggaactgc
     2701 gtggcaaaac gctgtacgaa gtgctgtatg ccaccccgga agtgagcaaa tacccggtat
     2761 ccgaactggc ggaagatcag ctgaacgatg aatcccgcga gctgggcttc tatctgcaaa
     2821 aagggctgtt cgaagagtac gcatggtttg gtcgcggtca cggtcacgat ctcgcgccgt
     2881 tcgatgacta ccacaaagca cgcggtctgc gctggccggt ggtgaacggt aaagaaacgc
     2941 agtggcgtta cagcgaaggt aacgacccgt acgtgaaagc gggcgaaggc tacaagttct
     3001 acggtaaacc ggatggcaaa gcggtgatct tcgcgctgcc gttcgaaccg gcggcggagg
     3061 caccggatga agagtacgac ctgtggctct ctaccggacg cgttctggag cactggcata
     3121 ccggcagtat gactcgccgt gtaccggaac tgcaccgcgc cttccctgaa gcggtcctgt
     3181 ttattcaccc gctggatgcg aaagcgcgcg atctgcgtcg tggcgacaaa gtgaaagtgg
     3241 tttctcgccg tggcgaagtg atctcgattg tcgaaacgcg cggtcgtaac cgtccgccgc
     3301 agggcctggt gtacatgccg ttcttcgacg ccgcacagct ggttaacaaa ctgacgctgg
     3361 acgcgaccga tccgctctcg aaagagacgg acttcaagaa gtgcgcggtc aaactggaga
     3421 aggtgtaagc cattatgtcc cggtcagcga aacctcaaaa tggtcgccgc cgctttctgc
     3481 gcgatgttgt tcgcacagca ggcgggctgg ctgccgtggg tgtggcgctg gggttacaac
     3541 agcaaaccgc acgcgcatct ggcgtgcggt tgcgcccgcc cggagccata aacgagaacg
     3601 cctttgccag tgcctgtgtg cgttgcggtc agtgtgttca ggcttgccct tacgacacct
     3661 taaaactggc gacgctggct tctggcctgt cggcgggcac gccatatttt gttgcgcggg
     3721 atattccttg cgaaatgtgt gaggacattc cgtgcgccaa agtgtgcccg agcggtgcgc
     3781 tggatcgtga gatcgaatcg atcgacgacg cgcggatggg gctggcggtg ctggtggacc
     3841 aggaaaactg tctcaacttt caggggctgc gctgcgatgt ttgttatcgc gaatgcccga
     3901 aaattgatga ggccatcacc ctggagctgg agcgcaacac gcgtaccggt aagcacgccc
     3961 gctttctgcc gacggttcac agcgacgcct gtaccggttg cggtaagtgc gaaaaagtgt
     4021 gcgtgctgga acaaccggca atcaaggtgt taccgctgtc actagcgaaa ggggagttag
     4081 gtcaccatta ccgcttcggc tggctggagg ggaacaatgg caaatcgtaa acgtgacgcc
     4141 gggcgcgagg cgctggagaa aaaaggctgg tggcgcagtc accgttggtt agtgttacgt
     4201 cgcctttgtc agttcttcgt gctggggatg tttttgagcg gtccgtggtt tggtgtgtgg
     4261 atcttgcacg gcaactacag cagtagcctg ttattcgaca ccgttccgct gaccgatccg
     4321 ctgatgacgc tgcaaagcct tgccagcggt catctgcctg ccacggtagc gctgactggt
     4381 gcggtgatta tcaccgtgct ctacgccctg gcaggaaagc gattattttg cagctgggtc
     4441 tgcccgctga acccgattac cgacttagcg aactggttac gcaggcgctt tgacctcaat
     4501 cagtctgcga cgatcccacg ccacattcgc tacgtgttgt tggtggtcat tctggtggga
     4561 tcagccttaa ctggcacgct catctgggaa tggatcaacc ccgtttctct gatggggcgc
     4621 agcctggtaa tgggattcgg cagcggtgcg ctgcttattc tcgcgctgtt tttatttgat
     4681 ctactggtcg ttgaacacgg ctggtgcggg cacatttgtc cggttggcgc gctgtatggt
     4741 gtgctgggta gcaaaggtgt gattaccgtt gcggccaccg atcggcagaa atgtaaccgc
     4801 tgtatggatt gttttcatgt ttgcccggaa ccgcatgtgc tacgtgcccc ggtgctggat
     4861 gaacaaagcc cggtgcaggt caccagccgc gattgcatga cgtgcggtcg ctgcgtggat
     4921 gtctgttctg aggatgtatt tacaataact acacgatgga gttcgggagc gaaatcatga
     4981 aaagccatga cctgaagaaa gcgctgtgtc aatggacggc gatgctggcc ctggtggtaa
     5041 gcggcgcggt ttgggcggct aatggcgtcg attttagcca gtcgccggaa gtatccggga
     5101 cgcaggaagg ggccattcgg atgccaaaag agcaggatcg gatgccgctg aactatgtga
     5161 atcagccgcc aatgatcccg catagcgttg aaggttatca ggtaacgacc aataccaacc
     5221 gctgcttgca gtgccacggt gtcgaaagtt atcgcaccac tggcgcgccg cgcattagtc
     5281 ctactcactt tatggacagc gacggcaaag tgggcgcgga agtggctccg cgtcgttatt
     5341 tctgtctgca atgtcacgta ccgcaggccg ataccgcgcc aatcgtgggg aataccttta
     5401 ccccatcaaa aggttacggg aaataagagg ttattatggg aaattctgac cgtaagcctg
     5461 gtctgattaa gcgcctgtgg aaatggtggc gtacccccag ccgtctggcg ctggggacgc
     5521 tgctgttgat cggttttgtc ggcggcatcg tcttctgggg cggctttaac accgggatgg
     5581 aaaaagccaa taccgaagag ttctgcatta gctgccacga aatgcgtaac acggtgtatc
     5641 aggaatacat ggattccgtg cactacaaca accgtagcgg cgtccgtgcg acctgtccgg
     5701 attgtcacgt tccgcacgag tttgtgccga agatgatacg caagctcaaa gcaagtaaag
     5761 agctgtatgg taaaattttt ggcgttattg acacgccgca gaaatttgaa gctcatcgcc
     5821 tgacgatggc acagaatgag tggcggcgca tgaaggacaa taactcgcag gagtgccgta
     5881 actgtcacaa cttcgagtat atggatacaa ccgcccagaa atcggttgcc gcgaagatgc
     5941 atgaccaggc ggtgaaagat gggcaaacct gtattgattg ccataaaggg atagcgcaca
     6001 agctgcccga tatgcgcgaa gtcgagccag gtttttaaca gggttattgc gtgggtatgc
     6061 ttgaagccag agagttactt tgcgagcggg atgaacgaac cttatttagt ggcttgtcat
     6121 ttacgctgaa cgcaggagag tgggtacaaa tcaccggtag caacggcgcg gggaagacaa
     6181 cgcttctccg tttgctgacg gggttgtctc gccctgatgc gggtgacgtt ctctggcaag
     6241 ggcagccctt gcatcaggta cgcgacagct accatcaaaa cctgttatgg ataggccatc
     6301 agccggggat caaaacccgg ctgacggcgt tagaaaatct gcacttttat catcgcgatg
     6361 gcgataccgc acaatgtctg gaagccctgg cgcaggccgg gcttgccgga ttcgaagata
     6421 ttccggtaaa tcaactttct gccgggcaac aacgccgcgt cgctttagcg cgtctgtggt
     6481 tgacccgtgc cacgttatgg atactcgacg agccttttac cgcgattgac gttaacggcg
     6541 tcgatcgtct gacccagcgt atggcgcagc atacggagca gggggggatt gtgattctga
     6601 ctacccacca gccgctcaac gttgctgaaa gtaaaattcg ccgcatttca ctgacgcaaa
     6661 cgggggccgc atgatgttct ggcgcatttt ccgtcttgag ctgcgcgtag cgtttcgcca
     6721 tagcgccgaa atcgccaacc cgctgtggtt cttcctgatt gtaattaccc tttttccgct
     6781 cagtatcggt ccggagccgc aactgctggc gcgtattgcg ccgggcatta tctgggttgc
     6841 cgcgctgctt tcatccttgc tggcactcga gcgactgttc cgtgacgatt tgcaggacgg
     6901 cagtcttgaa caattgatgt tgttgccgtt acccttgccc gccgctgtgc tggcgaaggt
     6961 gatggcgcac tggatggtaa ccggtctgcc gttactcatc ctttcgcccc tggtagcaat
     7021 gctactggga atggatgttt atggctggca agtgatggcg ttaacgctac tgctgggaac
     7081 ccctacgctt ggctttcttg gtgcgccggg cgtggcgctg acagtgggac ttaagcgcgg
     7141 tggtgtgctg ctcagcatac tggtgttacc gctgactatc ccattactca tctttgccac
     7201 cgccgcgatg gacgcggctt ctatgcattt gcccgttgat gggtatctgg caattttagg
     7261 cgcgttgctg gcaggcaccg cgacattaag tccttttgcg acggcggcag cgttacgaat
     7321 cagcattcaa taacgctgga ttactgattt ttgttcgagt ctggtatcga aactatgtgg
     7381 aaaacactgc atcaactggc gatcccacca cggctgtatc aaatctgtgg ctggtttata
     7441 ccgtggctgg caattgccag tgtggtcgtg cttaccgtcg gctggatctg ggggttcggc
     7501 tttgctccgg ctgattatca gcagggaaat agctaccgca ttatctacct gcatgtgcct
     7561 gcggcgatct ggtcgatggg catttatgca tcaatggcgg tggcagcgtt tattggcctg
     7621 gtctggcaga tgaaaatggc caacctggcg gtggcggcga tggcccccat tggtgccgtg
     7681 tttaccttta ttgccctggt taccggctct gcatggggaa aaccgatgtg gggcacctgg
     7741 tgggtatggg atgcacgtct gacttctgaa ctggtgctgc tgtttctgta tgttggtgtc
     7801 attgccctgt ggcacgcctt cgacgaccgt cgtctggctg gccgtgcggc aggtatcctg
     7861 gtgctgattg gcgtggtgaa tctgccgatt attcattact ccgttgagtg gtggaacacc
     7921 ctgcatcagg gatcaacgcg gatgcagcaa agtatcgatc cggcgatgcg ttcgccgctg
     7981 cgctggtcga tttttggctt cctgctcctg tctgccacgc tgacgctgat gcggatgcgt
     8041 aatttgattt tgctgatgga aaaacgccgt ccgtgggtga gtgaactgat actgaaaaga
     8101 ggccgtaaat gacccctgca tttgcttcct ggaatgaatt tttcgcaatg ggcggttacg
     8161 ccttttttgt ctggttggcg gtggtgatga ccgttattcc gctggtggtt ttggtcgtgc
     8221 actcggtgat gcaacatcgc gcaattctgc gtggcgtggc gcaacagcgg gcgcgtgagg
     8281 cgcgtttacg tgctgcgcaa cagcaggagg ctgcatgaat attcgccgta aaaaccgctt
     8341 gtggattgcc tgtgccgtgt tggcagggct ggcgctgact atcggtctgg tgctatatgc
     8401 gctgcgctcg aatatcgatc tcttttatac gccgggggaa attctctacg gcaagcgtga
     8461 aactcagcaa atgccggaag tcggtcagcg tctgcgcgtt ggcgggatgg tgatgccggg
     8521 gagtgtgcag cgcgatccca attcgctgaa agtgaccttc accatttacg atgctgaagg
     8581 ctcagtggat gtttcttacg aaggcatttt gccggatctg ttccgtgaag ggcagggcgt
     8641 tgtggtgcag ggcgagctgg aaaaaggcaa tcatatcctc gcgaaagaag tgctggcgaa
     8701 acatgatgaa aactatacgc cgccagaagt tgagaaagcg atggaagcta accaccgtcg
     8761 cccggcgagt gtttataagg acccagcatc atgatgccgg aaattggtaa cgggctgctg
     8821 tgcctggcgc tgggaattgc gctgctgctg tccgtgtatc cgctatgggg cgtggcgcgc
     8881 ggagatgcgc gcatgatggc gtcttcccgc ttgtttgcct ggctgctgtt tatgtctgtg
     8941 gctggcgcat ttctggtgct ggtcaatgct ttcgtggtca acgacttcac cgtcacctat
     9001 gttgccagca actccaatac ccagcttccg gtgtggtatc gcgtggcggc tacctggggc
     9061 gcgcatgaag gctcgctcct gctgtgggtg ctgctgatga gcggctggac ctttgcggtg
     9121 gcgattttta gtcagcgtat tccgctggat attgtggccc gcgtactggc gataatgggg
     9181 atggtcagtg tcggcttttt gctgttcatt ctctttacct ctaacccatt ctcacgcacg
     9241 ctgccgaatt tcccgattga agggcgcgat cttaacccgc tgttgcagga tccggggctg
     9301 atcttccatc cgcctctgct ctatatgggg tacgtcggtt tctcggtggc gtttgctttc
     9361 gccattgctt ctttgttgag tgggcgtctg gacagcacct atgcgcgttt tacccgcccg
     9421 tggacgctgg cggcgtggat cttcctgacg ctcggcattg tgctcggttc cgcatgggcc
     9481 tattacgaac tcggctgggg cggctggtgg ttctgggatc cggtagaaaa cgcctcgttt
     9541 atgccgtggc tggtggggac tgcgctgatg cattcactgg cggtcactga acaacgcgcc
     9601 agcttcaaag cgtggacatt actgctggca atcagtgcct tctcgttgtg tctgttgggg
     9661 actttcctcg tgcgttccgg cgtgctggta tcggtacacg cgtttgcctc tgatccggcg
     9721 cgcggtatgt ttatcctcgc ctttatggtg ttggtgattg gcggttcgct gctgctgttt
     9781 gccgcgcgtg gacacaaagt tcgctcacgc gtaaacaatg cgctgtggtc gcgggaatct
     9841 ctgctgttag cgaacaacgt tttgctggtc gccgcgatgc tggtggtatt actggggacg
     9901 ctgctgccgc tggtgcacaa gcaactggga ctgggcagta tttcgattgg cgaaccgttc
     9961 ttcaacacca tgtttacctg gctgatggtg ccgtttgcgc tgctgctcgg tgtcggtcct
    10021 ctggtgcgct gggggcgcga tcgcccacgt aaaatccgca atttgttgat tatcgccttc
    10081 atctctacgc tggtgctgtc gctgctgttg ccgtggctgt tcgaaagcaa agttgtggcg
    10141 atgacagtgc tcggcctggc aatggcctgc tggattgcgg tgctggcaat tgcggaagct
    10201 gcgctgcgta tttcacgcgg cacgaaaacc accttcagtt attgggggat ggtggcggct
    10261 cacctggggc tggcagtgac aattgttggc attgctttta gtcagaacta tagcgttgag
    10321 cgtgatgtgc gcatgaagtc cggcgatagc gtcgatattc atgaatatcg cttcaccttc
    10381 cgtgatgtca aagaggtgac cggtccgaac tggcgtggcg gtgtggcgac tatcggcgta
    10441 acgcgagatg gcaagccgga aacagtgctg tatgcagaaa aacgttatta caacactgcc
    10501 gggtcgatga tgaccgaagc ggcgattgac ggtggcatca cgcgtgacct gtacgcggcg
    10561 ctcggtgaag agctggaaaa cggcgcgtgg gccgtgcgtc tttactacaa accatttgtt
    10621 cgctggattt gggcgggcgg gctgatgatg gcgttgggcg gactgctgtg tctgtttgat
    10681 cctcgctatc gtaagcgcgt gaacccgcag aaaactgcgc cggaggctgt atgaagcgca
    10741 aagtattgtt aattccgttg attatcttcc tggcgattgc cgcggcgctg ctgtggcagc
    10801 tggcgcgtaa tgccgaaggg gatgatccga ccaatctgga atccgcgctt attggcaagc
    10861 cggtgccgaa gtttcgcctc gaatcactgg acaatccggg gcagttttat caggcggatg
    10921 tgctgactca gggcaaacca gtgctgctta acgtctgggc gacctggtgt ccgacctgcc
    10981 gtgcggaaca tcaatatctg aatcagcttt ctgcgcaggg cattcgcgta gttggtatga
    11041 actataaaga cgatcgccag aaggcaatca gctggctgaa agagctgggc aatccttacg
    11101 cactaagcct gtttgatggc gacggcatgt taggactgga tctcggtgtc tatggcgcgc
    11161 cagaaacgtt cctcatcgac ggcaacggca tcattcgcta tcgccatgcg ggcgatctca
    11221 atcctcgcgt ctgggaagaa gagatcaagc cactgtggga gaaatacagt aaggaggccg
    11281 cacaatgagg tttttattgg gcgtgctgat gctgatgatc tccggctcag cgctggcgac
    11341 catcgacgtg ttgcagttta aagatgaagc acaggaacaa cagttccgtc agctcactga
    11401 agaactgcgc tgcccgaaat gccagaacaa cagcattgcc gattccaact cgatgattgc
    11461 caccgacctg cgccagaaag tgtatgaact gatgcaggaa ggcaaaagta agaaagagat
    11521 tgtcgattat atggtggcgc gttacggcaa cttcgtcact tacgatccgc cgttaacgcc
    11581 gctgaccgtg ctgctgtggg tgctgccagt agtggctatt ggcattggcg gttgggtcat
    11641 atacgcccgt tcgcggcgtc gggtacgcgt ggtgccggaa gcgtttccag aacaaagcgt
    11701 gccggaaggt aagcgtgccg gatatgttgt ttatctgccg ggtattgtgg tggcgttaat
    11761 tgtggctggc gtcagctact accagactgg caattatcag caggtgaaaa tctggcagca
    11821 ggccacggca caggctccgg cgttactgga cagggcgctg gatccgaaag ccgatccgct
    11881 caacgaagaa gagatgtcgc gccttgcgct ggggatgcgt actcaactgc aaaaaaatcc
    11941 gggagatata gaaggctgga ttatgttggg ccgcgttggc atggcgctgg gtaacgccag
    12001 tatcgccacc gatgcatacg ccactgcgta tcgcctcgat ccgaaaaaca gcgatgctgc
    12061 actgggatac gctgaagcgt tgacacgttc atctgatccc aacgacaacc gcctcggtgg
    12121 tgaactgcta cgccagttgg tgagaactga ccacagcaat atccgtgtgc taagcatgta
    12181 tgcgtttaat gcctttgagc agcagcgatt tggcgaagcc gttgccgcgt gggagatgat
    12241 gtcgaaactc ttacctgcca acgatactcg ccgtgcggtg attgaacgta gtatcgcgca
    12301 ggcgatgcaa catttgtcgc cgcaggagag taaataaaaa gaggcccgat ggtgatgcca
    12361 tcgggctatt tttattgtgc cccgcgttgt tgcaggaaca gaatggtggc cgccacgcgt
    12421 gagcggacat tgagtttacg cagcagattg cgaatgtgta cttttactgt ctgctcggaa
    12481 atattcaaca ccgaggcaat ctgtttattt gacagcccct gtgccagctc gtgcagaaca
    12541 tccagctcgc gctccgtcag cacgctgaag ggatcttctt ctgcgccaaa catttcacgt
    12601 tcacgtaagt actgattgac gcgttcgcta aagactttgc tgcctttcgc tccggcacga
    12661 atcgcttcca gcaacacttc cgggtcgctg tctttcaaca gataaccgtc tgcgcctgcg
    12721 tctatcagcg caaagacatc gctggaggca tcggatacgg tcaggataat aatttgcgcg
    12781 gtaacgccat ccctgcgcaa ggcattgagg gtatccaggc cactcatacc tttcatattg
    12841 agatccagca agatcacgtc gatatccagt ctattcgcca gatcgatcgc gctcgcgccg
    12901 tcgcccgctt cggcgaccac ttcaaagcca ggatcaagct ccagtaactg acgaacaccg
    12961 cgtcgcataa gtggatgatc atccacaatc atcacctgaa aaggcgttgc ttcaggcata
    13021 gtagtctcct gaggttttat tagaatcatt atgtttgagc gtagcgttgg taacgctacc
    13081 aggcgcgtga atgttacccc attttatgta tggacatgta gcgaaaacta caaccttgtc
    13141 gttcgctttg gcatcatttt cattataaag acggaatttt gcatcagctt ctcatcaaga
    13201 aaaacaacta aaaaatcaat cacaatactt gtgcatttct ttaaacagtt tttaaaaact
    13261 gactccgggt atggagctat gggtattttc tctacccaat gcttttaaca gcaattaatt
    13321 tcataggatg aaagctcaat gcatcaatct ggttctgttt ctctttgtcg ttccgcaata
    13381 tctgttctgg tggctacagc gttatattca cccatagcat tggcatcaac agttgagtat
    13441 ggtgagacag ttgatggtgt tgtcctggaa aaagatatcc agctggttta tgggaccgcc
    13501 aataatacga aaatcaatcc tggcggagaa cagcatatta aagaatttgg tgtaagtagt
    13561 aatactgaaa ttaacggcgg gtatcagtac attgaaatga atggcaccgc agaatactca
    13621 gtattaaatg acggttatca aattgttcaa atgggtggtg cggcaaacca gactacgatc
    13681 aataatggag tgctacaggt ttatggcgcg gcgactgatc ccacgattaa aggagggcgc
    13741 ggtgatgcgg catttacgtt gggcaatgcc ggaagcgttg ttgatatcag tacgtatgaa
    13801 tataccttgc tggataatgg caaccatagc tggagtctcg cagagaatcg cgtacaaatg
    13861 cccccttcaa ccacggatgt gctgaatatg gcggcagcac aaccgctggt atttgatgca
    13921 gaactggaca ccgtgcgtgg gcgtcttggt agcgtaaaag gcgttaatta cgatacggcg
    13981 atgtggagtt cggcaattaa cagccgcaac aacgtgaaca ctgatgcggg agctggcttt
    14041 gagcaaacat tgacgggcct gacgctcggt atcgacagcc gtttctcccg tgaagaaagc
    14101 agcacaactc aggcgggtgt ggtctggacg ttctgataac agaaaataaa caggccgtga
    14161 tgtgtcgcgg tctgtttatc gaattaaatg cagatataaa aaaaccaacc gcaataggtt
    14221 ggttttcttg ggatttttgg tcggcacgag aggatttgaa cctccgaccc ccgacacccc
    14281 atgacggtgc gctaccaggc tgcgctacgt gccgactcgt ggctgctaat actaccgttt
    14341 tccacaccga ttgcaagtaa gatattttgc taactgattt ataattaatc agttagcgat
    14401 aaaacgcttc tcgtctgtca gtacttgcaa taacaaactt aactgtggtt tttcatcttt
    14461 cactctttca ccacgtaagt tgtaagtgcg gtatttaccg ttattgttca gcaccagtgt
    14521 ctttttcggg gtggtaattg ccagcgtatc gttatccgcg gcggtaaccc agtaatggcg
    14581 gcgttgaggg ttgaacaaat cctgaccttg cgaatattcg ctggcaggtg tgctgacatg
    14641 tagcaggcgt tgcatcagcg tcgtcatcag atcggtatga tcagtcagcg cattaatacg
    14701 ctgcgccggc gtgcctggcc agtgaatcac taatggcacc tgcagatgac cgtgggacca
    14761 gtcaaaggtt tcttcctctt cgctcagcgg aataccccgc ccggcagtga taatcaccac
    14821 cgtattgtcc agtttgccag aatcacgcag tgcattgagc acgcggttga tctggtcatc
    14881 gacattgcct gccgcccggc tatatttccg tgcaaatgcc tgctgattgc tgtcgtcaat
    14941 gtttgtgcca ttgaaagaga cccacgagaa ccagcggtta tcttcttgtg cgtagcggcc
    15001 cagccagttg atccactgcg tggcggtctg ttcgtcggat tgggtgcgta cactcggcat
    15061 cgagaaatct gacaacaatg cctgacgata cagcgggctg gtaaagccat ctgatgagaa
    15121 taaccccagc tgatagcctt gctgattaag cgcagtaatt aacgccgcag gcgtacgagt
    15181 cgacagaatg ccgtccatat agctcggcga gatgccatag aacaggccaa agatgccgtt
    15241 gtctgtagtg ttgccggagc tcatatggcg cgtgaacgaa atattttgct cagcaaaacc
    15301 tgccagcgca ggcatctgct tctcgaagcg tgagtagttc aggccatcga cagtaatcaa
    15361 cagcacattc tgcccggtgc ccatatcgcg atagcgcagt tcgcttaacg gatactgaac
    15421 ggaaacggcg tctggattac cttgctcaat aagacggcgt tgatattcct gcgcatcaag
    15481 caggccatgc ttctcaagaa aacgtcgcgc cgtcatcggg tacgaaagcg gcaggttagc
    15541 gcgctgcatg gtgatcgggc gatagaagtt ggcatcggcc cagatataca ccacatgcga
    15601 ggcgataaag gcgataaata agaatgcggc cagcgggcgc gcgaagcgcc gacgacgcgt
    15661 caggctgcgc agcttttgcc agctccacgt cgcaaacacc agttcaagca ataaaataac
    15721 cggcacgctg atgaacatca gctgccagtc gcgcgccatc tcattttcgt ctgggttgat
    15781 aaccagctgc cagacgatgg gattaagatg gagatggaaa cgagtaaaga cttcgctatc
    15841 gatcagtaat agcgtcattc ccgccgttgc cagaatgacg gacaaaaacc tcatcagcct
    15901 ctgggagccg acgataaagg tcagcgggaa gaggatcagc aagtaggtgg cgaacaccag
    15961 gaagctgaaa tgaccgataa tgcttacgta ggaataaatg cgaccagcaa gcgttgtcgg
    16021 ccagtcggcg ataaacaggt aacggctgcc aatgacgagc gaaagcagaa tattgaacag
    16081 tgcaaaccag tgcccccaac tgaccatctg ggagactttt tcacggtagc gctgacgatg
    16141 agttaccata acctgttatc tgtttccctt agtgcgcttt gtcttcgttg atagaggact
    16201 gtaaggcgcg ggcaaaagag ttggcaattg cctggcgttg ggccggggca atgctggtgt
    16261 tgataaggtt agtgaccatg ttgcctaaca ccatcaggga aaggtcggtc ggagccttat
    16321 gtttttccag tacgttgagc agctctgcga gcaattgttc aacctgttca tcactgtagc
    16381 gggaaatttg tggcataaat cgaaatcagt tagttgatga aagggcaaca tattaccgta
    16441 gcaacggttt tttttctgct tttttatgcg ttaaatcatt ctcgttacgc cgccttctgt
    16501 aaaatgaccg ccatctttta ttgcgtcgct taaccggcgg tggttgaata ccgcccggtc
    16561 ttaaaggaga gtttatcatg agtctggata tcaaccagat tgctctgcac cagcttatca
    16621 agcgtgatga gcagaacctt gagctggtgt tgcgcgattc attgctggaa ccgacggaaa
    16681 ccgtcgttga gatggtggca gaactgcatc gggtctatag cgccaaaaat aaagcttacg
    16741 gcttgtttag cgaagagagt gaactggcgc agacgctgcg cttacagcgt cagggcgaag
    16801 aggactttct ggcatttagc cgcgcggcaa ccggtcgcct gcgtgacgaa ttagcgaaat
    16861 atccttttgc tgacggtgga ttcgtgctgt tttgccacta tcgctatctg gcggtggagt
    16921 atctgctggt ggcagttctg agcaacctga gcagtatgcg tgttaacgaa aatctcgaca
    16981 tcaacccaac ccattatctt gatatcaatc atgcggatat tgttgcgcgt atcgatttaa
    17041 ccgagtggga aaccaatcca gagtcgaccc gctatctcac tttcctgaaa gggcgagtag
    17101 ggcgcaaagt ggcggatttc tttatggatt tcctcggtgc cagcgaaggg ttaaacgcga
    17161 aagcgcaaaa ccgtgggctg ttgcaagcgg tcgatgattt cactgccgaa gcacaactgg
    17221 ataaagcaga gcgacagaac gtgcgccagc aggtttatag ctactgtaat gagcagctgc
    17281 aggcaggtga agagattgaa ctggaatcgc tgtcgaaaga gctggcaggc gtcagcgaag
    17341 tgagcttcac ggagtttgcg gctgaaaaag gctacgaact ggaagagagc ttcccggcag
    17401 accgtagcac actgcgtcag ttgacgaagt ttgccggtag cggcggtggc ttgactatta
    17461 actttgatgc gatgctgtta ggtgaacgca tcttctggga tccagcaacc gataccctga
    17521 ccattaaagg cacgccgccg aacttgcgcg atcaattgca gcgccggacg tctggcggca
    17581 attaatcggt tttgtgttct gaatgccaga tgcggcgtga acgccttatc tggcctgcaa
    17641 aaaatagcaa taccgtaatc aggcataatt tgcagagaca aaaaaccccg ccggagcggg
    17701 gttttttaca acttattcag caattaagcg cgaacgaagt cgatgtgcag cagcttcggt
    17761 ttgtacgggt gacgctgtac gtcctgagct ttaactttga tttctttacc gtcaacaacg
    17821 atggtcagaa cttcgctgta gaattcagct ttaacttgca tgttcatgac tttgtcgtga
    17881 tccagttcga cagccagcgg cgcttctttg ccaccgtaga tgattgccgg gaacttgtta
    17941 gcggcacgca ggcggcggct cgcaccctta ccctgctctt tacgtacttc tgcgttgata
    18001 gtaaacattt ctttctctct taaattaatc ctgctacagg cgacccagca acaggtaagt
    18061 gatctgcttt gcgtatgcaa aagcgggcga gattctatac tcaatcgcct tatacatcaa
    18121 tcaaaactac cattaaccgc gtaattcgtg cgcccgacga aaacgacctt cgtaatcgaa
    18181 cactttttca cgcacttgcc agtactgacc tttcatgcgg gcgacgacaa aatccgggtg
    18241 tcgcagtaat gcttgctggg cgaggatatc ggcggcggtg atccagcgca gagggatgcc
    18301 cggtgtgcgc gtatgcgggc ggataaaaag ctgctcgaag gcggtacgct gggcgggcgt
    18361 ttgcagacgg aaacgctcac tcacatccgc gccgtcttca tcgtaatagg tgattttcaa
    18421 ccattcgcct ttctcgtcgt gtccatgctg caaagacatg ccgctacagc gtaataccag
    18481 cgcgtctttc agtcgtagcg ccgcttttaa catatcatcc ggatcaacca gtaccgtgtc
    18541 acattcgcgg cagcggcgag cggcaatatc attttctgcg ttacattgcg ggcaattttt
    18601 aaaacggaaa cggaagtcac actgttcgcg atgtccgtcg tcatcttcaa accatccctg
    18661 acagcgacga ccaaagtgtt caatcaatgt cccgtcggcg gtcgttttcc cccaaaaggt
    18721 gttggcaaaa ccgcaggcag ggcagaaaac ctgtaccgga acgttgtcac ttttgccttt
    18781 cggtgtacca acttccggcg cgtagagatc gtgagggtta cccgcataat caagaattaa
    18841 gcaatcagtc ttgccggggg ccaggcgcag accgcgcccg acaatttgtt ggtaaagact
    18901 cactgattcg gtagggcgca gaatggcgat aagatcgacg tgcggggcgt caaatccggt
    18961 ggtcagtacc gcgacgttga ccagatagcg aaaacgctgg gctttaaaat catcaattaa
    19021 cacatcgcgc tcagagccgg gtgtgtcgcc agtaatcagt gctgcatctt cggcaggcag
    19081 taatcccaca atctcttttg cgtgttcgac cgtcgcggcg aaaatcatca cccctttgcg
    19141 cgttgcagca aactccatga tttggctgat gatgtgtggc gtaatgcgtt gctgtttttt
    19201 cagctcgcgg ttgagatcgg cttcgctgaa cagtccgtta ctctgtgcct gcaagcggct
    19261 gaaatcgtat tgcacgactg gcatatccag ccgttctggc ggcgtcagat agccgtgttt
    19321 aatcatataa cgcagcggca gctcataaat gcagtcacgg aaaagggctt tctcatcgcc
    19381 gcgtaccatg ccgtgataat ggaactgata aatccagcct ttgcccagtc gaaaaggcgt
    19441 ggcagtcagc cccagcaggc gtaagtgggg attcactttg gtcaggtgag tgaggatttg
    19501 ctgatactgg ctctcttcgt cgtcaccaat acggtggcat tcatcgacaa tcaacagcga
    19561 aaattcaccc tgaaaggcat caagattacg ggcgaccgac tgcacgctgc caaataccac
    19621 tttaccgtgg ctctctttgc gctttagtcc ggcggcaaaa atatcggctt ccagtcccag
    19681 tgcctgatat tttgcatggt tttgcgccac cagttcttta acgtgtgcca gcaccagcac
    19741 gcgaccacgc gccagccgcg ccagttccgc tatcaccagg cttttacctg caccggtggg
    19801 cagcacgata acggcagggg ttttatgacg acgaaaatgg ttgagcgtgg catccacggc
    19861 ttcttgctga tatgggcgaa gtgtaaaaat catggtctcg cgtaggtaga ttattctgct
    19921 aatagtatgc catggatctt tttcccttga gggatgttgt tagaccgcta tactgggcag
    19981 acaacagttc agattttcgg gtcattaccc gacctccgtt ttttacaggc aaaatcacac
    20041 acatgcgact tgataaattt atcgcacagc aactcggcgt tagccgtgct attgccgggc
    20101 gtgaaatccg cggcaatcgt gtcaccgtcg atggcgaaat cgtccgtaat gcagcgttca
    20161 aactgctccc tgaacatgat gtcgcttacg atggcaaccc gctggtgcag caacacggtc
    20221 cacgttactt catgctcaat aagcctcagg gctatgtttg ctccacggac gacccggatc
    20281 acccaacggt gctttatttt ctggatgaac cggtagcgtg gaaactgcat gctgcagggc
    20341 gtctggatat cgataccacc ggtctggtac taatgacaga tgatggtcag tggtcgcacc
    20401 gcattacttc cccgcgccat cattgcgaga agacctatct ggtgacgctg gaatcacccg
    20461 tagctgacga tacggcagag caatttgcta aaggcgtgca gctgcataac gaaaaagatc
    20521 tcactaagcc ggcggtgctg gaagtgatta ccccaacgca ggttcgtttg accatcagcg
    20581 aagggcgtta tcatcaggtg aaacgtatgt tcgccgccgt gggtaaccat gtggttgagc
    20641 tgcatcgtga acgtattggc ggcattacgc tggatgctga tttagccccc ggtgaatatc
    20701 gtccgttaac tgaagaagaa attgccagcg tcgtctaact tttcaatgac tttcaggagc
    20761 ccgttgtgac cacccgacag cattcgtcgt ttgctattgt ttttatcctt ggcctgctgg
    20821 ccatgttgat gccgctgtcg attgatatgt atctgcctgc gctaccggta atttcagctc
    20881 agtttggtgt accggcgggc agtacgcaga tgaccctcag tacttatatt ctgggctttg
    20941 cgttggggca gttaatctac gggccgatgg cagacagctt cgggcgtaag ccggtggttc
    21001 ttggcggtac gctggtgttt gccgccgccg cggtggcgtg tgcgttggca caaaccatcg
    21061 atcagctgat tgtgatgcgt ttcttccacg ggctggctgc ggctgcggcc agcgtggtta
    21121 tcaacgcttt gatgcgtgat atctacccga aagaagagtt ctcacggatg atgtcgtttg
    21181 tcatgctggt gacgaccatt gcaccgctga tggcaccgat agttggcggc tgggtgctgg
    21241 tgtggctgag ctggcattac atcttctgga tcctcgccgt ggcggcgatt ctggcttcgg
    21301 caatgatctt ctttctgatc aaagaaacct taccaccgga gcgtcgccag ccttttcaca
    21361 ttcgtaccac tattggtaac tttgcggcgc tgttccgtca taagcgtgtc ctgagttata
    21421 tgctggccag cggtttcagc tttgccggga tgttctcatt cttaagcgcc ggaccgtttg
    21481 tctacattga aatcaaccac gtcgcgccgg aaaactttgg ttattacttt gcgctaaaca
    21541 ttctttttct gttcgtgatg accatcttta acagccgctt cgtccgccgt attggcgcgt
    21601 taaatatgtt ccgctcgggg ttgtggatac agtttattat ggcagcgtgg atggtcatca
    21661 gtgcactgct ggggctggga ttttggtcgc tggtggttgg cgttgcggcg tttgtgggct
    21721 gcgtgtcgat ggtgtcatcc aatgcgatgg cggtcattct tgatgagttt ccccatatgg
    21781 cgggaacggc atcttcgctg gcaggaacct tccgtttcgg cataggggca attgttggcg
    21841 cattactttc tcttgcgacc tttaactctg catggccgat gatttggtca attgcattct
    21901 gcgcaaccag ctccattctc ttctgtttgt acgccagtcg gccgaaaaaa cggtgatcta
    21961 ttgcacaacg aggaagctaa aaggcttcct ttgttgatgc atgtcaacca caaatctatc
    22021 attcccccga tatatgttta ttttatgtaa aatcaattta tgtaaaaagt cacatcattg
    22081 tagttaaaaa ggttgagtta gatcgcagaa acgggtacat atagccccgc aaacgtgacc
    22141 acgcccgcag atattattta aatcagagcc atagaggcca cgcaggcgag gcatcaatct
    22201 ttacgatctg tataaagacg gattgttgat gatgtgttaa aattgatgta aacaaattgt
    22261 gaagtaaaat gtacttccgg ggaaaataag tgacttcatt acaactctca atcgtccatc
    22321 gactgccgca gaactatcgc tggtctgctg gtttcgcagg ttcgaaggtt gaaccgattc
    22381 cgcaaaatgg accgtgcggt gacaacagcc tggtggcgct taaattgctt agcccggatg
    22441 gtgataatgc atggtcggtg atgtacaaac taagccaggc gttaagcgac atcgaagttc
    22501 catgttcggt gctggagtgt gaaggtgagc cgtgcctgtt tgtaaatcgc caggacgaat
    22561 ttgctgcaac atgccgattg aaaaattttg gtgtggcaat tgccgaaccc ttttcaaact
    22621 acaatccttt ttaagcgtca gctcaacgcc agtagctgac gcgtatactc ctgctgcggt
    22681 gcggcaaaca cgcgcgcgca cggtccttgt tccactactt ccccttgtcg cagtacgata
    22741 acctgatgac ataacgcgcg gacaacgtgc aaatcgtggc tgataaacaa ataggccagt
    22801 tgatgctttt gttgcaatga tttcaatagc gtcaatattt gcgcctgcac cgttttgtcg
    22861 agtgatgatg ttggttcatc aagaatgatc agcgagggct taagaattaa cgccctggca
    22921 atcgcaatac gttgccgctg accaccagag aactccgccg gataacggtg gcgtgtttca
    22981 ggatctaatc ccacttcatg catcacggct atcacttgtt gttcgcgttg cgcggcagaa
    23041 agcgtcggct ggtgaacccg taagccttcc tcaataatct gcaaaacgtt gagtcgtggg
    23101 ttgagcgagg agtttggatc ctgaaatacc acctgaatgc gatgacgaat aggtaacagc
    23161 tggcggcgat ttaaattttg cagtggctta ccgtcaaaga cgatgctgcc ctgagagtta
    23221 atcagtcgca gcagcgccag tcccgtcgtg cttttcccgg aaccagactc gcccactaaa
    23281 cccagtgttt cacccgctcg tagcgtaaaa ctgatgtttt tcaccaccac attatgatcc
    23341 acaatgcgct tcaaaatccc tttgcgaatg gggaaggcaa cctgaagctg ttcaacatcc
    23401 agcaacgttg aggcaggttc tggcaatggg accgggtcgc ctgacggttc actgttgagt
    23461 agcttttgtg tgtaaggatg agtgggtgag gcaaatagcg tagcggcgtt attttgctcg
    23521 acacagcgac cgttttgcat taccgccacg cggtgggcca gttttctgac aatgctgagg
    23581 ttatgagtaa taaacagcat acccatattc agctcgcctt gcagttcgcg caacagctgt
    23641 aaaatctgcg cctggacaga gacgtccagc gcggtggttg gttcatcggc aattaataat
    23701 tccggtcgcg ttaacagcgc catcgcaatc atcacccgct ggcgttcgcc gccggagagc
    23761 tgatgcggat aatctgtcag ccgttttgcc gcctggcgga taccgacgcg atcaaggcag
    23821 ttaagaattt cgccacgagc cgcttcccga cgcatcccgc ggtggagtga aagcacttca
    23881 taaagctgtt tttccagggt atgcaatgga tttaacgaca ccatcggttc ctgaaaaatc
    23941 atggcaatct tattaccgcg tacaccgcgt aacgtttgat cgctggcatg aagcagcgat
    24001 tcgccatgaa aacgaatatc gccggagaga tattcaaccg gcggggaagg gagcaggcgt
    24061 aaaattgaca gcgcggtaac gcttttgcct gaacctgact cacccaccag cgccagcgtt
    24121 tcgccagcct caatctgaag tgaaacatca ttgactactg tgcgtacggt ttgctgatgg
    24181 cgaaaaccca ccgacaaatt ttcaatcgct aacagagttt gcgtcatgct acaccgcctt
    24241 attaggatca aatgcgtcgc ggacggcttc accaataaag atcagcaaag acaacaatat
    24301 cgccaccgac aagaaggcgg tgatcccaag ccacggggcc tgaaggttat ttttcccttg
    24361 taacagcagt tcgccgagtg acggtgaacc gagcggcaga ccgaagccga ggaaatcgag
    24421 tgaggtcagg gtggttatcg aactacataa aataaacggt aaaaaggtga gggtagcgac
    24481 catggcattt ggcaacatat gacgcaggat gatactgcga tcgctgacgc caagcgcctg
    24541 cgccgcgcgg atgtagtcga aattacgggt acgtaaaaac tccgcccgca ccacgccgac
    24601 cagactcatc cagccaaaca agacggtaat tgccagcagc caccagaagt taggctgtac
    24661 gacgctggaa agtaaaataa tcaaaaacag cgtcggcatc cctgaccata cttcaataaa
    24721 gcgttgcccc cagagatcga ctttaccgcc gtaatagcct tgtagcgccc ccgccagcac
    24781 gcccatcacg ctggaacaga gagtcagcat caggccaaac agaaccgaga tccgcgtgcc
    24841 atagagaatg cgcgccagca catcgccgcc attggcatcc gttcccagcc agttttgccg
    24901 ggagggtgga gaagggaagg gcttatcggt agcaaagttg atactggtag caccaaagcg
    24961 aatcggtgcc cacagtaccc agccgttatt ttccagccgt tgtttcagcc acgggtcctg
    25021 ataatcagcc tgacttgcca gcgggccgcc aaaatcgctt tcgctgtagt ttttcaacaa
    25081 cgggaaatac caactgccgt cataacgcac cagcaacggt ttatcgttgg cgataagttc
    25141 agaacacaaa ctcaaaccaa acaagacgag gaaaatccat aacgaccagt agccgcgacg
    25201 gttatgacga aaacgcgccc aacgggcctg attgacgggg ctgagtcgcg acattaacgt
    25261 ccctcaaaat ctatacgcgg atcgaccagc gtatagctga tatcactgac aatattcagc
    25321 agcaggccaa tcagggtgaa aatatacaag gtaccaaaca ttacaggata atcgcgcgag
    25381 acggtcgctt cgtagcccag taagccaaga ccattgagtg aaaacatcac ctcaatcagc
    25441 agcgagccgg taaaaaacat gctgataaac gtcgccggaa aaccggcaat caccagcagc
    25501 atggcgttgc ggaacacatg tttccagaga atattttttt cacttacccc tttcgcacgt
    25561 gccgtcacca cgtattgctt acgcacttca tcaaggaatg agttttttgt cagcatggtc
    25621 agagccgcaa agccgccaat cactgtcgcc agcaccggca gcgtgatatg ccacagataa
    25681 tcggtgattt tctgatacca cggcagcgaa tcaaagttag cggaaaccag gccgcgcaga
    25741 gggaacaggt cgaaatagct gccgcccgcg aagaagacaa tcagcaggat ggcaaacaaa
    25801 aaggccggaa tggcgtagcc gatgatgata aatgcactac tccagacgtc aaaacggctc
    25861 ccattataaa cagctttgcg aatgcctaac ggaatcgaca ccagatagat aatcagcgtg
    25921 ctccacaatc cgagggtgat ggagaccggc agactgtctt taatcagcgt cagcaccgag
    25981 gcgctgcgaa aaaggctgtc gccaaaatca aaacggatgt agtcccagag cattttgaag
    26041 taacgttcgt ggatcggctt atcgaaaccg tagcgatgag tgatctcggc gatcacttct
    26101 ggatctaatc cgcgtccgcc acggtaatta ctgtcgctga tattaccgac gcccgtttgt
    26161 gcatggctgg cgcgaacacc ttcaccgcct gcaccgggta atacaccagt attaccaaac
    26221 tcaatggcgg cgatggcctg gtcgaccgga ccgccaggcg caatttgcac gatgaaaaag
    26281 ttgatagtga taatcgccca taacgttggg atcaccagca acagacggcg aatcagataa
    26341 gcgcccatct actctccctg ttgtctggct gacggcagtt tggccgcttt attaacgtca
    26401 taccaccagg tatcgatacc caaactgtaa acagggcgta cagcgggttg ggagaattta
    26461 tcccaccagg cgagacggtc ttccgccatg taccacattg gcagcatgta ataattccac
    26521 gttaatacgc gatccagtgc tcgccccaac ggcagtaatt tttctttatt tccctgcgcg
    26581 gcaatgattt ggttgatcag cgagtcgata accgggcttt gcacgccggg ggcattataa
    26641 gtggaattga tatattccga tgaccaggaa atctgtaaat cggaactggg ccacggcatc
    26701 gcccgccata cgcgcggcat catgtcatag tcgcgactgc gcatgcggtt ggtgatttgc
    26761 gagttatcca ccttgcgaat gtccatgttg atacccagcc gttgcaggct gtgctggaac
    26821 ggcaataccc actgactatt gctgcttgcg ggaagcaata attcaaagct gagtggctga
    26881 cccgttgtga cattaacgcg ttgctgaccc ttcagcaccc agcctgcttc gttgagaagt
    26941 ttgtcggctt ttaacaggtt gtcacgatcg tagccatcgc ctttggatac cggcggctgg
    27001 tagatttgcg tgaagacttc tggcggtaga tcttttttca ttggtgccag aagcaccagt
    27061 tccgcggcgt cggggtaatt tttggccgca tattcggtat tctgaaaata actgttcgtg
    27121 cgactccagg cattgtaaaa caacgctttg ttcatccatt caaagtcaaa ggcgagagtg
    27181 atcgcttccc ggacccggcg atcgctgaat accggacgtt ggatattaaa cgccagccag
    27241 cgcgtatcct gggctgattc gttcttttgc tcatctttga tgatgtattt tttatcgaaa
    27301 tttttaccgg tatagcgcgt ggcccagttt ttggcgtcgt tttccatacg caaatcaaag
    27361 gcacctgctt taaacgcttc aaaggcgaca ttatcatcga ggtaataatc gtagcgaatg
    27421 gtgtcgaaat tccagcgtcc acggtttacc ggtaagtttg ctgcccagta atctttcacg
    27481 cgggaataga caatattttg ccccattttc caggacgtaa tgcggtacgg accactggca
    27541 agcggaggcg tggcgagcgg gtcgctaagt ttgtgatctt tccagtactt ttctggaaat
    27601 accggcagcg aaaacagact cagcatatct tctttgccgg gtttagctaa ctcaatgcgc
    27661 acggttaacg gcgcaatggc tttgacggtg gtgcctttgt agaccagacg aaattgcggc
    27721 acgccttcgg tcataaattt ttgaaaagta aactctacat cgcgggcagt aatgggcgaa
    27781 ccatcatgaa aacgggcgcg cggattaata gcgacctcca cccaggaata atcgtcagca
    27841 tagcgtgcgc tttcagcaat cagcgggtaa taactgcctg gttcgtcatc ggaagtcgta
    27901 aatagcgtgt cgtacagctg ctcggtgcgt gcgcccgggt tgccgcgtag tgcatagcgg
    27961 ttgaaattat cgaaggtgcc gagggcggac aacgttattt gcccaccttt tggcgcggcg
    28021 gggttcacat aatcaaaatg gttgaaatta aatgcgtacc ggggttcgcc cagtacggca
    28081 aaggcatagc tttccttgat agcctgcgcc tgcgcaccaa aggtgaacag agcgataaac
    28141 agcagcagta tgcgcacaat catctgcaac ggatttcctt ttactcatca ttagccgcaa
    28201 tccggcatgc tttcactatg aataataagg gacgtaagca caccttacca ctgcggcgta
    28261 tacggtttct tcagccagcg aacaaaatcg tccagcggta acgggcgact aatccagtag
    28321 ccttgcatga aattaacgcc gcgttcgctt agccatcgtg cctgttctgg cgtttcgacc
    28381 ccttcagcaa ctgtcagcat attgagacgt ttcgccagtg tcagcaccgc gtcaagtacg
    28441 ggtgaagtga tcgtttccgt accgatggcg ttgataaatc cacgatcaat tttcagataa
    28501 tcgagcgtaa aacgctcaag atagataagc gcgctgtgcc cggtgccgaa gtcatcaata
    28561 gcaatttcta cgccgaccga gtgcagccag gcgaagagtt gtgtggcttc tcgctctttc
    28621 agcatatcgc gctcggtaat ttccagcaca atctggaaat ggtgtgcggg cagggaagtg
    28681 agcagtttct ggatatccgc tttaaagctt tcgctgtgca agtgggccgg cgcaatgtta
    28741 atgccaaatt tgacgcctac cggcagtact ttttctaatt ctgcggcatc gcgggcaatc
    28801 aactcaaaca ggtgctgagt cagtggcaca atcatctttt gcgcttcggc aaagttaatg
    28861 aaggcatccg gggggatttc acctgctact ggatgccgcc agcgtagcag tacttccagg
    28921 cccgttaccc gcaaagcttg tgtatcaacc accggttgat acaccacgta aaattgttcg
    28981 cgcttgatgg cggtcatgat ttctctgcct gggcgcatac gcacactcat caggtaatag
    29041 cagagaagtc caacgaaagt tccactcatg ccacccagta aaaatgcgta ccacacatcg
    29101 ttccatgtcc agtcatctgc ataaagccga acggtcagag gaatgcccgc aattttagtt
    29161 tcacggaccg gcatgtcggt taattcatta acgttcatca aacgtgaaga aaaggtagat
    29221 agcgcagtat taccaataat gagggcgacg ccatcgtaat cttcctgccg tgaactataa
    29281 aagagagaag gcgtcagatt gagattcagc gcggcaaaca cgccactatt tttcaacaac
    29341 gggttgcgat accagatgac gattgcgggt ttgttcggca ccatcggcgt accgggtaag
    29401 atcgccatat cgacattttt attaatgtcg agcgccggaa tcaattcttt tagtggaatg
    29461 tccatctcac cagtcgcaga tgaacagaat gtttttttat ctttcaccag cacaaacgta
    29521 cggacattca tgctaaacgc cgcgcgggca gtcagttcgg ggttagcctg ctggcaggta
    29581 tctaaggtca gcggctggag ccggtcagta gtggatttaa ggtcggcaaa ataggtatcg
    29641 agatactttt gtacgtcggt aatcagtgtg tcgtatttga cttcgtgctt atgccaggcc
    29701 actaaaaact gaaggcagct caccagtatc gcaatcatca cgcctgcaac aatgcaggta
    29761 agcaggagct tacgtccaga attgggagcg cgtataaaca tgattccgtt aatttcctga
    29821 aattacgaca acatttaaaa gataacaatg accggactat agcctggatc aagacgcgga
    29881 aacatcattt tctctgatga atgaaaacaa aaaagcactg cctaagcagt gctttcagtt
    29941 aactcgtcag gatagccaag ggattgcatc caaacggttt attagctgcg gctgagaacc
    30001 cggcgtgctt cgttgtaacg cttcttccag tacggttcat tcatgctgga aataataaca
    30061 ccactgctgg tggaagcatg gacaaactga ttgttgccga tataaatacc gacatggcgt
    30121 cccgttgaac cggcacggaa cagaactaaa tcacccgtac gcaaattact gcgggaaacc
    30181 gatttaccca tttcctgctg ttcgtaagtc gaacgcggaa gttctaagcc aaattgctca
    30241 cggaatgtac gctgtacgaa accagaacaa tcgatacctt ttttagtgct gccgcccaga
    30301 cgatagcgta cgcctttcca gtcagcatac tgatccataa ttcgcgattt tacgtcgaca
    30361 ttacgaacca ggttttcaaa ttcatcctga gaagcttgca gtgatgatgt ttcactaccc
    30421 actgcacgtg tctcaggatg catattcttt gcggtgttat ttgcactaca tgcagaaagc
    30481 agaaccgcta ctgcaatcgc ggggatcccg cgcaagatat atctcaaaat cggttgagat
    30541 ttgaccatgt tgtttgtttt cccttgaagt ccttaacgac aaatatcgtt ataaaaaaat
    30601 gccaagcgac acgagactaa ttacctgggc atgttgaaac aattccttaa ggccaaatct
    30661 gtggactaac gcacaaaatt atatgctgtg aggataatct accgccgagg caaaacgagt
    30721 tcgagattac ccgattggtg cggtcttggc gagcggtttt acatgatttt ttataagaaa
    30781 aagtgataca gaaagttaat aagcgggatt ggtcaattaa agtacgattg agtgaagcgc
    30841 atgcaatcca ttcatttact tagagaatga tttttgcgta cgatggatta aagcaggggg
    30901 gcatcatcag gattatcctc tctatgaaaa taaacctgat gatgttaatt actgtgagtt
    30961 atttgttttg gtaatgtttg ttttttcccg gtagtgattt gtcaaaaaag gtgattaatc
    31021 gatcacttaa tggcgtcagc aacacccagg gcaaaccgat caatatcacg gtcatcgaac
    31081 cgacaatgat gtcagtaaac cagtgtgcgc caatcattac tctgggaaac gcaaacacca
    31141 caaaaataat aagggcgata aggcctgcaa ctttgccgaa ataacgccac atgaatgccg
    31201 aaaaaataag cagcatcata ccgtgatcgc cgggaaagct atctcgtgag gcatctttcg
    31261 tgggaacaga gagcagttcg ctgacgcggt taatatcggt aaaagtcaat gttgggctgg
    31321 cccgttttac aggaattaat gcctgaccca gctggtttaa taccactgca gttaatagca
    31381 tgaccagacc aataatcacg atacgtcgtc tgcctggggc gttttctttc agccagaaac
    31441 tcagcatcaa cattcccatc gccagcagtg aacaaccgtc gaaagcgcga ttgttggtca
    31501 atgcaaccag ccacaaaaag gccttacttt cgaccagttt ctgattaaaa aaataaaaaa
    31561 tatccgcatc aatcggcaac cagaatccat gattaacggg aatataccag gaaagaaaca
    31621 gcgcgaggcc gacaatattc aacaacacta tttgcggcaa atttttaatc atattttctc
    31681 taattatctt actgaaaaac gcaggcaacc ttacgcggtg gtcgctaaac gaagcttcaa
    31741 cagcgcgctc tgcaaggcat tccagtcagc ttcgctgctg gaaatcagct caatacgact
    31801 gtccggtggc gcaacgtttt gcgtttcaat gtgcaggtca tcgccctgac ggttgattcg
    31861 caccagccct tcgggaatac gcatcacgcc tttgacgcgt tccaccggtg caagtcgcgc
    31921 ccattccaga atgccaatgg tgtcgaatac cgtatcagcg tcgaatatcc agccacaggc
    31981 ctgatatcct tgcccgctgt tcagactgcg acgccagcgt tgatgctctg gcaggcttaa
    32041 cgctgctaac ccttttttca cgccatgctg atgagaatgc gcggcgctgg cgggcaactc
    32101 ggctaaattg cgacgcggca aatccagaag atgaccgtca attttcccat gctcgctgtg
    32161 aattaattgc cgatcgccac cattttgctg ccaccagcgc tgtagcgctt gctcactttc
    32221 gggcgtcgca cggtcggatt tattggcgac aataatgtct gcggcagcca gctggtcacg
    32281 gaagttttca ttgctggcgc ttttttcgtc cagcagcagg cgtggatcga gaatgcacaa
    32341 cgtggcgcgc agatctatcc acggttcata cacgggcgcg gttaagagat ctaggatctg
    32401 tttcggatga cccagtccgg tcggctctat caacaagcgg tctggttttc cctgacgcag
    32461 taaggtattc aatcctacct gcatgggtaa accattaacg cagcacatgc agccgccggg
    32521 gatctctttc agcaatgcgc cgctatcggc gagcaaagca ccatcaattc cgacttcccc
    32581 aaattcatta accagtaccg cccatttttc gttgggatct ttatgggcta acagatgaag
    32641 aatcgacgtg gttttcccgc tgccgagaaa accggtgatg aggttggtcc tggtcacaac
    32701 ggctccgcaa tactcttaaa agttaactga gcgttaatcc tggctaaaaa cggcagaaaa
    32761 gagaagggga gagcggacga aggcgagctt catccgcttt aaccgcagaa atcgttaaat
    32821 tttgagggtg tttaatagcg cctgatgcgc gccgaattgt gcgatttgtt gccaggcttg
    32881 ttcgatggcc tgcacaaaat gcgggttatc tggcagatca tcaccgaaaa cttcacgcag
    32941 ggaaagcagg gcggtgacgc gttgctcact gctgctgccc gcaacaagat cgcgaatttt
    33001 atcgctaagc ggatcgcgaa catcaatggt atttccggca tcatcaacgc cgctgacgta
    33061 acgcatccag cctgcaacgc ctaatgccag caacgaccag tccgtttcgc gcgccagatg
    33121 tatgcgaata cctgccagca tacgttgcgg taatttctgg ctaccatcca ttgcgatttg
    33181 ccaggtctta tgtttcagcg ccgggttagc aaaacgtgca attaacttat ccgcatattg
    33241 tgttaaatcg acatctttaa tccgcagtgt cggcgcttgc tcatccagca ttaatgttct
    33301 ggcggcatgg cgaaatgcgc gatcctgcat gcaatcactg atgtgcgcga atcctgagag
    33361 gtaacccaga taagcgagaa aagagtggct gccattaagc atacgcagtt tcatctcttc
    33421 ccatggcagg acatcattca ccatttgtac acctgcgact tcccaggcag gacgcccagc
    33481 gacgaagtta tcttccacca cccactggat aaacggttcg cagctaatcg cacagggatc
    33541 attcaccccc agatgctggc tgatttccgc cagtgattcg tcggttgctg ccggaacaat
    33601 acggtcgacc atggttcccg gaaaactgac gtgttctttt atccacccgg cgagttctgg
    33661 tgaacgtttt tccgccatcc ccagcaccgc gtttttcacc acatggccat tgtcgggaat
    33721 attgtcgcag gagagtacgg taaacggtgc aaggccgcgt tcacggcgtc gtttcagtgc
    33781 ttcgacgaga atacccggcg cggagtgagg ttcttcagga ttttgaagat cgtgaataat
    33841 ccgcggattg ctggtgtcga gtgcaccggt ggctgggtca atacaatagc ctttttcggt
    33901 aatcgtcagg gaaacaattg ccacctgtgg ctcgcaaaat ttctcaataa ttgccgctaa
    33961 ggaatccagt tttgcattaa ggcattcgtg aacggcaccg acaattatcg cctgattgcc
    34021 gtccgcccct ttctccagca cggtatataa atggttctgt gcgcggagct ggctcatcag
    34081 ttgatcaccg ctgaacaagc tgatttcaca gatcccccag tcgccgccct gggcattcag
    34141 cacacgatcg gtcagtaacg cctgatgagc gcggtgaaag gctccaaagc caaaatgaac
    34201 gatacgtgat tgcaactgct ggcgatcgta gcgtggagcg tgtacatgat gcgggagcgt
    34261 aacggaggcg attgtattca taggagtcac gtcttagtaa cgattaatta acaatgacag
    34321 tgtaaagtta tatgacagca attgaaattg gtctggattg ttaatgcgat gaagtgtgag
    34381 ggcgatcaat caacgagtga atattgcggg tgtaaggtgt tcaggaagcg caaaatagaa
    34441 gattggtata ataaattaaa aaagggacag cctgagctat cccttttctg catgcggctg
    34501 aagttagtca gcacgcccca tataacggcg ttcttcgata tggatacgaa ttttttcgcc
    34561 cgggcttaag tattctggta cctgaatcac cagaccagtg ctcaatgtcg ccggtttgtt
    34621 acgggcgctg gcagatgccc ctttgatgcc tggtgcagtt tcaacgattt ccagatcaac
    34681 ggtctgcggc agctcaagcg ccagcagttg accgtcccag gtcagcacct gcatgtccgg
    34741 catgccgccc tcaggcataa acagcaactc ttcttcaatc tgatctttgg tgaaggtata
    34801 cggggtatag tcttctttat ccataaagac atattcgttg ccatcgacat aggagaagtc
    34861 aacgtaacgg cgggtcagcg tcacggtgtc aacgatatca tcacctttga aacgctcttc
    34921 tactttcagc ccggtacgga catcagaaaa acgcattttg tacagcgtag cggcaccgcg
    34981 ggcagtgggc gactgaatat caatgtcctt aaccagcagc agtttgccgt tgtaattcag
    35041 taccatacct tttttaattt cgttcgctct tggcatcgaa aaattcctgt agtcgaaaat
    35101 gtcaaaaata tcgcgacaaa gtactcgcga cgggcctttc gggcaagtgg tattcgcact
    35161 tttgctggtg caaaaaaggt ggtactgtgc gcgctcatca atccggtggt taaccttaag
    35221 agaacaacgt atggaatgcc gtccgggttg cggggcgtgt tgcactgccc cttcaatttc
    35281 cagccctatt cccggtatgc cagatggcaa gcccgccaat acgccctgca ttcagcttga
    35341 tgaacagcaa cgctgtaaaa ttttcacctc gccactgcga ccgaaagtct gtgcaggctt
    35401 acaagccagc gctgaaatgt gcggtaactc gcgtcagcag gcaatgacct ggcttatcga
    35461 tctggaaatg ctgaccgcgc cttaaacatc tttaattcgc agtaagcaaa acagagtagc
    35521 gataatcatc accatcgcaa accagaacac agcgtgataa ttccagatct cggcgacgat
    35581 gcccgccact gatcctgcga tgatccagcc cacgcgcgac gtgttggtat cgagcgtggt
    35641 ggctgaacct gcctgaccgg gcatcaaatc ctgaaaatag agcatcccga tgccgcccag
    35701 aatgccaata aaaatagcat ttagcagctg caagcccaac agaatgacag gtgaatgcgc
    35761 catcagcatt cctgcgtaaa aacagacgcc acccacggca gcaacgcgca ttaagaagcg
    35821 cttacccaga cgtttggcaa aatatccggc gatcaacatg gtcgggattt ccagccctgc
    35881 ggcggtcccc atcatcacac cggccagttt ctcgggaaga tgcagttcgt tgataataaa
    35941 tagcggcatg ttgatgatgt acaggctgtt cgagccccac atcaatgtac aaatgacaaa
    36001 cagcagcaac gtatcgcgac ggttacggcg cggcgcttca accgtgccgg tcgccagcgg
    36061 aagctctttt tgcatcgacg gcaaaaacag ccacaccatc acgccgcaaa caataaacgc
    36121 taccgctgcg ctcagataca ttaccgtaaa gctgaaaccc atcgctaagg cataagccag
    36181 cggtgggcca atgacccatg ccagtgaaac ctgagcgcgt aaaaaagagc tgaacatcac
    36241 cgcctcacgt ccggttttgt cagcatgttc acgggcaagg gcaaacattt gcgggttagc
    36301 ggttgagcca aagctgctaa gaaagacgcc aacgaatagc aaaacaaagt agttgcgatt
    36361 ccaggcaaaa agggtgcagg ccagcacgcc taacaggcag caaaagacaa tcagcgattt
    36421 gcgatcgccg cgcttatcag agcgcccggc gagaaactga ctcaccagaa tcccaatgac
    36481 agcgctgccg gtaaagaaga atcccaccat cgccggacgg gcatgtactt catcagtaag
    36541 aaaaatactg agtgtcgggg tttgcagagc gcccgcaata ccggtgagaa aggcaacgat
    36601 taaaaacgcc gtcgaggtca ggtcaaacga tttcgcgctg gtgactgcgg gggagttatg
    36661 catgctggtc gatcactgga agaggaagac gcggagttta cgctgcttgt cagaaaataa
    36721 acagacagcg aaatcaaaag cgccacgaat aatcaaaatg ccatttcact gttgctgatt
    36781 ggctgctgac gctgctgaaa tgaaattgct gataacggat tttcccatca gcaattagga
    36841 aaaatgacaa aaaattgtgc agcacatcaa acttttgctc ataactttac ggctttcctt
    36901 gcgtgctgaa acaaaagtat tatgctttct tgaaacgttt cagcgcgatc ttgtctttaa
    36961 ccctaagcca ggttggcgct tttttctcat agaagctgaa tcgtttcaat tcagcaagag
    37021 aggagaacta tgttccagtt atccgtacag gacatccatc cgggcgaaaa ggccggagac
    37081 aaagaagagg cgattcgcca ggtcgctgcg gcgctggtgc aggccggtaa tgtagctgaa
    37141 ggctacgtca atggcatgct ggcgcgcgaa cagcaaacct caacgttcct cggcaatggt
    37201 attgctattc cacacggcac taccgacacc cgcgatcagg tgctgaaaac cggcgttcag
    37261 gtatttcagt tcccggaagg cgtcacctgg ggtgacggcc aggtagcgta cgtggcgatc
    37321 ggtattgctg ccagctcgga tgaacatctg ggcctgctac gccagctgac ccacgtactg
    37381 agcgatgatt ccgttgctga acaactgaag tcagcaacaa cagcagaaga acttcgcgca
    37441 ttgctgatgg acgaaaagca gagtgagcag ctgaagctcg acaacgaaat gctgacgttg
    37501 gatatcgtcg ccagcgatct gctgactctt caggcgctga acgctgcgcg tctgaaagag
    37561 gcgggggcag ttgacgccac tttcgtcacc aaagccatca atgaacaacc gctgaacctc
    37621 gggcagggta tctggctgag cgatagcgcc gaaggcaatc tgcgtagcgc gattgcggta
    37681 agccgtgcgg caaatgcttt tgatgtggac ggcgaaacgg cagccatgct ggtgagtgtg
    37741 gcgatgaatg acgatcagcc catcgcggtt cttaagcgtc tcgctgattt gttgctcgat
    37801 aataaagctg accgcttgct gaaagcggat gcggcaacgt tgctggcgct gctgaccagc
    37861 gatgatgcgc cgaccgacga cgtgttaagc gcggagtttg tggtgcgcaa tgaacacggc
    37921 ctgcatgctc gtccaggtac catgctggtc aataccatta aacaatttaa cagtgatatt
    37981 accgtgacaa accttgatgg caccggcaaa ccggcaaacg gacgtagtct gatgaaagtt
    38041 gtggcacttg gcgttaagaa aggtcatcgc ctacgcttta ccgcccaggg tgcagatgct
    38101 gaacaggcgc tgaaagccat tggcgacgct atcgctgctg gtcttgggga gggcgcataa
    38161 tgagtagacg tgttgctacg atcaccctta atccggctta tgaccttgtt ggtttctgcc
    38221 cggaaattga acgcggcgaa gtgaacctgg tgaaaaccac cggtctgcat gcggcgggta
    38281 aaggcatcaa cgtggccaaa gtattaaaag acctgggaat tgatgtcacc gttggcggct
    38341 tcctgggtaa agacaatcag gatggttttc agcaactgtt cagcgagctg ggcattgcca
    38401 accgtttcca ggttgtacag gggcgcaccc gaattaacgt taagctgacg gaaaaagacg
    38461 gcgaagtgac cgacttcaac ttctcgggtt ttgaagtcac ccccgccgac tgggaacgct
    38521 ttgtgactga ttctctgagc tggctcggtc agttcgatat ggtctgtgtc agcggaagct
    38581 taccgtcagg cgtcagcccg gaagcgttca ccgactggat gactcgcctg cgtagtcagt
    38641 gtccttgcat tatctttgat agtagccgtg aagcgttagt agcaggtttg aaagcggcac
    38701 cgtggctggt gaaacctaac cgccgcgagc tggaaatctg ggcaggccgt aaactgcctg
    38761 aaatgaaaga tgtgattgaa gctgcgcatg cgctgcgtga acaaggcatc gcgcatgttg
    38821 ttatttcact gggtgccgaa ggcgcgcttt gggttaatgc ctccggcgaa tggatcgcca
    38881 aaccaccgtc agtcgatgtc gtaagcaccg ttggcgcagg ggattctatg gttggtggcc
    38941 tgatttatgg cttgctgatg cgtgaatcca gtgaacacac actgcgtctg gcgacagctg
    39001 ttgcagccct ggcggtaagt caaagcaatg tgggtattac cgatcgtccg cagttggccg
    39061 caatgatggc gcgcgtcgac ttacaacctt ttaactgaca gcaggagagg cataatgaaa
    39121 acgctgctga ttattgacgc taatctcggt caggcacgcg cctatatggc gaagaccctg
    39181 ctgggcgcgg cggcgcgaaa agcaaaactg gaaatcatcg acaatccgaa cgacgcagaa
    39241 atggcgattg ttctcggtga ttccatcccg aatgacagcg cgctgaacgg taaaaatgtc
    39301 tggctgggtg atatttcccg ggcagttgcg caccctgagc tgttcctgag tgaagccaaa
    39361 ggccatgcga aaccttacac tgcgccggtc gctgcggcag caccggttgc cgccagcggt
    39421 ccgaaacgcg tagttgcggt gactgcttgc ccgactggcg tagcacacac ctttatggcg
    39481 gctgaagcca ttgaaaccga agcgaaaaaa cgtggctggt gggtgaaagt tgaaacccgt
    39541 ggttcggttg gcgcgggtaa tgcaatcact cccgaagaag tcgcagcagc ggatctggtg
    39601 attgtggcgg cagatatcga agtggatctg gcgaaatttg ctggtaaacc gatgtatcgc
    39661 acctctaccg gtctggcgct gaagaaaacc gcgcaggaac tggataaagc ggttgctgaa
    39721 gcaacgccgt atgaaccggc gggcaaagct caaacagcga ccactgaaag taagaaagag
    39781 ggtgcaggcg cttaccgtca cttgctgacg ggcgtctctt acatgctgcc gatggtcgtt
    39841 gcaggtggtc tgtgtatcgc gctttctttt gcttttggta tcgaagcgtt taaagagccg
    39901 ggtacgttgg ctgcggcgct gatgcagatt ggtggtggtt cagcctttgc gttgatggtg
    39961 ccggtactgg caggttatat tgccttttcc attgccgatc gtccgggtct cacgccgggt
    40021 ctgattggcg gtatgctggc ggtcagcacc ggttctggct tcattggcgg tattattgcg
    40081 ggcttcctgg ctggttacat tgcgaagtta atcagtacgc aattgaaact gccgcagagt
    40141 atggaggcgc tgaaaccgat cctgatcatt ccgctaattt ccagtctggt ggtcggtctg
    40201 gcgatgatct acctgatcgg taaaccggtt gctggcattc tcgaagggtt gactcactgg
    40261 ctgcagacca tggggactgc gaatgcggtt ctgctggggg cgatcctcgg tggcatgatg
    40321 tgtactgaca tgggcggtcc ggtaaacaaa gcagcgtacg cattcggtgt gggtctgctg
    40381 agtactcaaa cctatggccc gatggcggcg attatggcgg caggtatggt gccaccgctg
    40441 gcaatgggtc tggcaacaat ggtggcgcgt cgcaaattcg acaaagcgca gcaggaaggt
    40501 ggcaaagccg ctctggtact tggtctgtgc tttatttcgg aaggtgcaat tccgtttgct
    40561 gctcgtgatc cgatgcgtgt gctaccgtgc tgtatcgttg gtggggcgct gactggcgca
    40621 atctcaatgg cgattggtgc gaaactgatg gcaccacacg gtggtctgtt tgttctgctg
    40681 atccctggcg ctattacgcc ggtactgggt tacctggtag ccattattgc cggtacgctg
    40741 gtggcgggtc tggcctatgc cttcctgaaa cgtccggaag tggacgcagt agcgaaagca
    40801 gcgtaataaa aggtgtgtta cagggcagaa atttcctgcc ctgtaacatt tgatgcgcgc
    40861 aaagagaatg accggtttgt gaatgtaatt ttatgaattt aaatggtttt atcttttagt
    40921 agaaacctga aaccttcatc aaacactaag gttatttcct caagggcaga ttaatttctg
    40981 ccctttttta ttgttcctgt tctgtgcatc ccgtcacaaa ttccccgttt ttataattca
    41041 ggcatttaat cgcgttttgt gagcctgctc aaatctgccc attttgcctc tctggcggta
    41101 atttacttta cgtaaaacat gcatactatg agcacatgtt taaatatgaa cacatgttta
    41161 aaccgtcttt gcagtatcaa tattcttgtg gattgacagg gttggtaatt ttttaccaga
    41221 ggacgtgcta tgcgcgaaaa ggattatgtc gtaattatag gttcggcgaa tattgatgtc
    41281 gccggatatt cacatgaatc attaaattat gcggattcaa atccaggtaa aataaaattt
    41341 acgcctggtg gagtcgggcg caatattgca caaaacctgg cgttgctggg gaacaaagcc
    41401 tggctactga gcgccgtagg cagtgatttt tatggtcaat cgctgctaac gcaaaccaat
    41461 caatctggcg tttatgtcga taaatgcttg attgtgccgg gagaaaatac gtcaagctat
    41521 ttatcattac tcgataatac cggtgaaatg ctggttgcta taaatgacat gaatattagc
    41581 aacgctatta cagctgaata tctcgcacag caccgtgaat ttattcagag ggcaaaggtc
    41641 attgtcgcag actgtaatat cagtgaagag gcactggcat ggattctgga taatgccgcc
    41701 aatgtacctg tatttgtcga tccggtttcg gcatggaaat gtgtcaaagt gcgcgaccgt
    41761 ctaaatcaga tccacaccct gaagccaaac cgccttgaag cggaaaccct gagtgggatt
    41821 acgctgtcag ggcgtgaaga tgtggcaaaa gttgctgcct ggttccatca acatggcctg
    41881 aaccgaatgg tattgagcat gggcggcgac ggcgtttatt acagtgatat cagcggtgaa
    41941 agtggttggt ctgcgccgat caaaaccaat gtcattaatg ttaccggagc gggcgatgcc
    42001 atgatggcgg ggcttgcttc gtgttgggta gacggaatgc cgtttgccga atctgttcgt
    42061 ttcgcacagg gatgttcgtc aatggcgctc tcctgtgaat acaccaataa ccccgattta
    42121 tcgattgcca acgttatatc gttagtggag aacgcagaat gtctgaatta aaaatttccc
    42181 ctgaattatt acaaatttcc ccagaagtgc aggacgcttt aaaaaacaaa aaaccggttg
    42241 tggcgctgga atcgaccatt atttctcacg ggatgccgtt cccacaaaat gcccagaccg
    42301 caattgaggt tgaagaaact attcgtaaac agggtgctgt acctaccact atcgccatta
    42361 ttggcggcgt gatgaaagtg ggtttaagca aagaagaaat tgaattgctg ggtcgtgaag
    42421 ggcataacgt gaccaaagtt agtcgtcgcg atttaccttt tgttgttgcc gccggaaaaa
    42481 atggcgcaac caccgttgct tcaacgatga ttattgcggc gcttgccgga attaaagtat
    42541 ttgccacggg gggaattggt ggtgtgcatc gcggggcgga acataccttc gatatttctg
    42601 ccgatttgca agaactggca aatactaatg tcaccgttgt ttgtgccggg gcgaaatcta
    42661 ttctcgattt aggcttaact accgaatatt tagaaacttt cggtgtaccg ctaattggct
    42721 atcagaccaa agcgctgcct gcgtttttct gccgcaccag cccgtttgac gtgagcattc
    42781 gtctcgacag cgccagcgaa attgcccgtg caatggcggt gaaatggcaa agcgggctga
    42841 acggtggcct cgtggtagcg aacccgattc cggaacagtt tgctatgccg gaacacacta
    42901 tcaatgcggc gatcgatcag gcggtagctg aagctgaagc gcagggtgtt attggtaaag
    42961 aaagtacgcc attcctgctg gcgcgcgttg ctgagttgac cggcggtgac agcctgaaat
    43021 ccaacatcca gctggtgttc aacaacgcca tcctggcgag tgaaatagcc aaagaatacc
    43081 agcgtctcgc gggttaattt ctgacgggca gaaagccgtc gcccgtactc atcaagcata
    43141 ccaaaacctg gcgctgacgc cgggtttact ggcatgaagg gaaaatcaat atggatataa
    43201 tgagaagtgt tgtggggatg gtggtgttac tggcaatagc atttctgttg tcagtgaata
    43261 aaaagagcat cagtttgcgc acggttggag ccgcgttgct gctacaaatc gccattggcg
    43321 gaatcatgct ctattttcca ccaggaaaat gggcggtaga acaggcggca ttaggcgttc
    43381 ataaagtgat gtcttacagt gatgccggta gcgccttcat ttttggttcg ctggtcgggc
    43441 cgaaaatgga tgtcctcttt gacggtgcgg gttttatctt cgcctttcgc gtacttccgg
    43501 cgattatctt cgtcactgcg ctcatcagtc tgctgtacta cattggcgtg atggggctgc
    43561 tgattcgcat ccttggcagc attttccaga aagccctcaa catcagcaaa atcgaatctt
    43621 ttgttgcggt taccactatt ttcctcgggc aaaatgagat cccggcgatc gttaaaccgt
    43681 ttatcgatcg catgaatcgc aacgagttgt ttaccgcaat ttgtagcggg atggcgtcca
    43741 ttgctggttc gatgatgatt ggttatgccg gaatgggcgt accaattgat tacctgttag
    43801 cggcatcgct aatggcgatc cctggtggta ttttgtttgc acgtattctt agcccagcca
    43861 ccgagccttc gcaggtgaca tttgaaaatc tgtcgttcag cgaaacgccg ccaaaaagct
    43921 ttatcgaagc ggcggcgagc ggtgcgatga ccgggctgaa aatcgccgct ggtgtggcga
    43981 cggtggtaat ggcgtttgtc gcaattattg cgctgatcaa cggcattatc ggcggaattg
    44041 gcggctggtt tggtttcgcc aatgcctctc tggaaagtat ttttggctat gtgctggcac
    44101 cgctggcgtg gatcatgggt gtggactgga gtgatgccaa cctggcgggt agcctgattg
    44161 ggcagaaact ggcgattaac gaattcgtcg cttacctgag cttctcccca tacctgcaaa
    44221 cgggcggcac gctggaagtg aaaaccattg cgattatctc ctttgcgctt tgtggttttg
    44281 ctaactttgg ttctatcggt gttgtcgttg gcgcattttc ggctatttcg ccaaaacgcg
    44341 caccggaaat cgcccagctt ggtttacggg cgctggcagc agcaacgctt tccaacctga
    44401 tgagcgcgac cattgccggg ttctttattg gtttagcttg agtgtcattg ccggatgtac
    44461 gcgcttatta agcgtcacat ccggcaatcg ctctattgca aataattttt actgcatcat
    44521 ccctgagaat ttattttccg ggtccatctc cagtgccagt cctgacaact gctttctgtt
    44581 tttaattaga taccctttct tgcttttaat taataaaccg tcgtgaatga attgcgcgag
    44641 aacatataaa agatgtcgat aagaaacacc taaatactct gccgcttgcg tatgcttttc
    44701 gtgataaaga tcaccctcct gcgagagtaa tataaaagct gccaggcgat tcactaacgg
    44761 aaatgattga ttctgagtta aagaaacaat attacgataa tttttatgac ttaaggtgac
    44821 gcagagtttt cgtaaaaata gcgtgtcgtt taataacagc gggcggtaat gtttcatagg
    44881 gagcgcaagg caccaacact catcaatagc ctgcaccgca cgcggttcat ggtctttgtc
    44941 gattaactca atctcgccaa taaaacaggg agcggcaaag aaatcgatca gcgacacgcg
    45001 accattagca agcgtggcgt aaagcctggc gcggcctcgc gtcagataaa acagccatga
    45061 cggctgttgc ccttcctgca caatgtagtc acgcgctaaa aaatgaaaca gccgcgtatc
    45121 ggctgaaaca tccgttgaaa agcaatcctt aaacgcagat tcactcatca attgctgctt
    45181 aaggtcatta ttgtggattt ctttcacgat gtttatccgc tatgagattt ctcatattta
    45241 gcatacttac gctgttgtta agattcaatc atcggcccga tcgaattttc tttgctgtag
    45301 aaaaaaaaac gcaactggaa acagaggaaa taaacaatgg aaaagagaaa aattattctg
    45361 gattgtgatc cgggtcatga tgatgctatt gccataatga tggcagcgaa acatccggcg
    45421 attgatttat taggtatcac cattgtggcg ggtaatcaga cgcttgataa aacattaatt
    45481 aatggcctga atgtttgcca gaaactggag attaatgttc cggtttatgc ggggatgcca
    45541 cagcccatta tgcgtcaaca aatcgttgcc gataatattc acggtgaaac cggactggat
    45601 ggcccggtat tcgagccgct gacccgccag gcagaaagca ctcatgcggt gaaatatatc
    45661 attgataccc tgatggcaag cgatggcgat atcactttgg tgccggttgg cccactttca
    45721 aatatcgcgg tggcaatgcg tatgcaaccc gcgatcctgc cgaaaatccg tgaaattgtg
    45781 ctgatgggcg gcgcttacgg tactggcaac ttcaccccat ctgccgagtt caacatcttt
    45841 gctgacccgg aagccgcacg cgtggtgttc acctccggcg ttccattagt gatgatgggc
    45901 ctcgatctca ccaaccagac cgtttgcacc ccggacgtga ttgctcggat ggaaagggca
    45961 ggcggccctg ccggagagct gttcagcgac atcatgaact tcactctcaa aacccagttc
    46021 gaaaactacg gcctggctgg cggcccggtg catgacgcca cctgcatcgg ttatctgatt
    46081 aaccctgatg gcattaaaac ccaggagatg tacgtcgaag tggacgttaa cagcggccct
    46141 tgctacgggc gtacggtctg cgacgagctg ggcgttcttg gcaagcccgc caataccaaa
    46201 gtcggcatca ctattgatac tgactggttc tggggattag tcgaagagtg cgtgcgcggc
    46261 tacatcaaaa cccattaagt gttgataaca agccgggaca gtatcgcaag atgcaggccc
    46321 ggctatttct gatgggcaga aagccgtcgc ccaaaatgac atgaagagat aataactatg
    46381 gatgtcatga gaagtgttct gggaatggtg gtattgctgg caattgcgtt tttgctgtca
    46441 gtaaacaaga agaagatcag cctgcgtacc gttggcgcgg cgttagtgtt acaggtcgtg
    46501 attggcggca ttatgctttg gttaccacca gggcgttggg tcgcagaaaa agtcgctttt
    46561 ggcgtgcata aagtgatggc gtacagcgat gcaggtagcg catttatctt tggttctctg
    46621 gtcggaccaa aaatggatac cttatttgat ggcgcaggat ttatctttgg tttcagggta
    46681 ttaccggcaa ttatcttcgt tacagcactg gtgagtattc tctactacat cggtgtgatg
    46741 gggatcttaa ttcgcattct cggcggtata ttccagaaag cattgaatat cagcaagatt
    46801 gagtcattcg tcgcggtcac taccattttc ctcgggcaaa acgaaattcc ggcaatcgtc
    46861 aaacccttta tcaatcgtct gaatcgcaat gaattattta cagcgatttg tagcgggatg
    46921 gcctcgattg ctggttcgac aatgattggt tacgccgcac tgggcgtgcc tgtggaatat
    46981 ctgctggcgg catcgttaat ggcgatcccc ggcgggatct tgtttgcccg cctgttaagc
    47041 ccggcaacag aatcttcgca ggtttccttt aataacctct ctttcaccga aacaccgcca
    47101 aaaagcatta ttgaagccgc tgcgacaggg gcaatgaccg ggctgaaaat cgccgcaggt
    47161 gtggcgacag tagtgatggc atttgtcgcc atcattgcgt taattaacgg tattatcggc
    47221 ggcgttggcg gctggtttgg ctttgaacat gcctcgctgg agtccatttt aggttacctg
    47281 ctggccccac tggcgtgggt aatgggtgtt gactggagtg atgcgaatct tgccgggagt
    47341 ttgattggac agaaactggc aataaatgaa tttgtcgctt acctcaattt ctcaccttat
    47401 ctgcaaacag ctggcactct ggatgctaaa accgtggcga ttatttcctt cgcgttgtgc
    47461 ggtttcgcta actttggttc tatcggggtg gtggtggggg cgttttctgc ggttgcgcca
    47521 caccgtgcac cggaaatcgc ccagcttggt ttacgcgcgc tggcggcggc gacactttct
    47581 aacctgatga gtgcaaccat tgcaggattc tttattggct tagcgtagtg cgcgagttat
    47641 gagccggata agatgtagtc aagcgtcgaa tccggcaaca ttgttacacc atcggcacta
    47701 atgaaagcgc gttatcggca gacagggtag ggttgtttaa gctgccgctg gcgcgtgaaa
    47761 ttgccgcgca ggccatcgca aaacgggcgc tgtcacggaa attgtttccc tccagaaagc
    47821 tatataccag gcccgccata aaaccatcgt cagcaccaaa actgtctact gtcgtatgcg
    47881 ctggcgctgt cagcaaaaat tgctctccat ccttttcgct gcaataaact gactcatcgg
    47941 gcaaataaac aaacagttgc tgaacgcctt gctgatgcaa cgcattcact gcggcattac
    48001 ggtcagcatc gctggtgatc gcctgtcccc ataaaatttc cagctccggt aaagtgggtt
    48061 tcagggtgtg aatatgcgcc agccagtgtt tgattttgcc cgctttgaat tctgaaacgg
    48121 tatcgacaaa caccgggatt tcatcagcga gggtaaagac ccattccagc gcctcggctg
    48181 tcaggttaca atcagcaagt accacgcccg catgacgaag taaatcgcgc gacccgttca
    48241 gtagttgcgg tgtcaactga tccagcagat gggtatcgtt aatcgccagc acggtttgat
    48301 cgtctcgatt ggcaattgcc agatacgtcg atgtgctttg gccatgtaaa cgaacgcagc
    48361 cggagacatt cacgccggcg cggcgcgttt cttccaggag catttcgcca taaaagtcat
    48421 cgccaatcac tgaaagcaaa tggacgtcgc ggcctaacag cgccagattg tgggcgatat
    48481 tgcgtccaac gccgcctgcc gagcaatgaa tggtaccggg atgagaagcc gcttgcgggt
    48541 aacggatatc cgccatcccg cgaatatcca tattgattgt ccccaccact acgcagtatt
    48601 cctgctcggt gagaatgtaa cctttgcctt taatccggcc tttgcgcatt aaatccataa
    48661 tatgcgccgc aacgcgcgaa cggctgattt gcagcatatc cgcaatttcg ttctgttgaa
    48721 tcagcgggtt acgccgtaaa attttaagaa tctgcttttc cctgtcgttc atcgatcagg
    48781 ccaccgcttt ttcagtttgt tgcgctttca gccaggcgat ctcttctgcc cagatatccg
    48841 ggttgatggt ttcaaggatc agcggaatgc cgtcgaaacg gtcgtcctgc atgatccagc
    48901 ggaacgcatc atgaccgata ttgccttcac cgaggctatg atggcggtca acgcggctgc
    48961 caaaggtgct tttcgcatcg ttaaggtgca tcccgcgcag atacttaaag ccgacaatac
    49021 gggcaaaatc cgcgaacgtt ttctcgcatt cggcagaagt acgcaaatca tacccggcag
    49081 cgaaagcatg gcaggtatca atgcagacgc cgacgcggga tttatcttcc acgccgtcga
    49141 taatcgccgc gagatgttcg aatttaaacc ctaagttact gccctgaccg gcggtgtttt
    49201 caatcaccgc tgtcacacct tgagttttat ccagcgcaat gttgatggat tcggcaatac
    49261 gcgcaaggca atcctcttct gaaatctgca tcagatggct gccagggtgg aagttgagca
    49321 aagaaagccc cagctgttcg caacgctgca tttcatctat aaacgcatcg cgcgattttt
    49381 ccagagcttc agcgaccgga tgtccgaggt taatcagata actgtcgtgg ggaagaattt
    49441 gcgccgatgt gtagtgatat ttttcacagg cggctttgaa ttcatcgatg gtttgcgtcg
    49501 tgagcggtgc ggcacgccac tgacgttggt ttttggtgaa caaggcaaac gcggttgcgt
    49561 cgatttcggc ggcgcgaatt gcggcatttg ccagaccgcc agcagcacta acgtgcgctc
    49621 caatgtattt catgcgagga ctcctgttaa acccgctgga ggaaaacgat aatgatagcg
    49681 ggttaacagg agcaagatgt agtggtttat gcgatgacgc tttgaatcac atagttaatc
    49741 gcaccaccac caacaatcag ccaggcaaac agtaccagtg ccatcagcag aggtttcgcc
    49801 ccggcttttt tcagcgcgct gacgtgagtg gtcagaccca gcgccgccat cgccattgcc
    49861 agcaggaagg tatccagcgt taccagcatg ttcaccacgc tctgcggtaa caggtggaac
    49921 gagttaaaga tggcaactac gatgaacaag atggcaaacc acggaatagt gattttgctt
    49981 ttctcgccgc tgttcgcccc agacagctgt ttaacacgcg ccgccagcag gatgaggaac
    50041 ggagccagca tcatcacgcg cagcattttg gaaataactg ctgcgttttc tgcatccggg
    50101 ctgatggcat gacctgccgc caccacctgc gccacttcgt gcacagtaga accaatgtag
    50161 ataccgaaag tttccggact aaaccattga gacatcagcg gatatatcgc cgggtagagg
    50221 aaaatcgcca cggtcccgaa gataacaacg gttgcaacag ccacggttac tttactggct
    50281 tccgctttca ctaccggctc agtcgccagt accgcggcag caccacagat actgctaccg
    50341 gcaccgatca accagctggt gtgcttatcc agaccaaaca ctttctgccc caggaagcaa
    50401 gccagcagga aggtactgga cagcgtcaac acgtcaatga tgatcccact gataccgaca
    50461 tcggcaattt gcgagaacgt cagacggaag ccataaagaa tgatacccag acgtaataaa
    50521 tattgcttgg caaacagcac accaccgtca cagcttttcc agatgtgcgg atagatggtg
    50581 ttgcctaaaa ccatccccaa caagattgcg agggtgaggg cactaaaccc ggcacccgca
    50641 accgcgggaa tggaaccacc ccacagggcg accccggtga taactgcact cagggctaac
    50701 cccggaataa aatgccacag tgtacgatgt tgtttctgta aggtgatatt cgtcataacc
    50761 ctctccttta accgggcata aggttacgac taactggttt aaaaataaaa ttgattatat
    50821 atttataatt aatctttata agtggtaagc gactatgcac atcaccctcc ggcagttgga
    50881 agtttttgca gaagtattga aaagtggatc aaccacccag gcgtcggtga tgctggcgtt
    50941 gtcgcaatca gcagtgagcg cagccttgac cgacctggaa gggcagcttg gcgtgcaact
    51001 gtttgatcgc gtggggaaaa gactggttgt taatgaacac gggcggctgc tctatccgcg
    51061 cgcgttggca ctgcttgaac aggcggttga aatcgaacaa ctgtttcgcg aagacaacgg
    51121 cgcgattcgt atctatgcca gtagtactat cggtaactac attctgcctg cagtcattgc
    51181 ccgttatcgc catgattatc cgcagttgcc gattgaactt agcgttggga acagccagga
    51241 cgtgatgcaa gcggtgctgg atttccgcgt tgatattggc tttattgaag gaccgtgcca
    51301 cagcactgaa atcatttctg aaccgtggct ggaagacgag ctggtggttt tcgccgcgcc
    51361 gacttcgcca ttggctcgtg gtccggtcac cttagaacag ctggccgctg cgccgtggat
    51421 cctgcgtgaa cgcggctccg gcacgcggga gattgtcgat tatctgttgc tgtcacattt
    51481 accgaagttt gagatggcga tggaattagg taactccgag gcaatcaaac atgcggtgcg
    51541 tcatgggttg ggaattagtt gcctgtcgcg acgggtgatt gaagatcaat tgcaggcagg
    51601 cacattaagt gaagttgcgg tccctctgcc gcgcctgatg cgtacgttgt ggcgtataca
    51661 tcatcggcaa aaacaccttt ccaacgcgct aaggcgcttt ctggactatt gcgatcccgc
    51721 gaatgtaccg cgttaagttg ctgtacaaga acatgctggt gctgtgtcga tttcgtgacg
    51781 cagcgccctc agcatgcatt cgccagaaaa gagattggct gctttactta taatccctgg
    51841 gcgatcatga aggtgtctta taaccgtgta tttctgccgg aaggattgcc aatcgtctgc
    51901 tacaatcgcg cctcattttt aagatggata gcatttttgt atggtttccg aaactaaaac
    51961 cacagaagcg ccgggcttac gccgtgaatt aaaggcgcgt cacctgacga tgattgccat
    52021 tggcggttcc atcggtacag gtctttttgt tgcctctggc gcaacgattt ctcaggcagg
    52081 tccgggcggg gcattgctct cgtatatgtt gattggcctg atggtttact tcctgatgac
    52141 cagtctcggt gaactggctg catatatgcc ggtttccggt tcgtttgcca cttatggtca
    52201 gaactatgtt gaagaaggct ttggcttcgc gctgggctgg aactactggt acaactgggc
    52261 ggtgactatc gccgttgacc tggttgcagc tcagctggtc atgagctggt ggttcccgga
    52321 tacaccgggc tggatctgga gtgcgctgtt cctcggcgtt atcttcctgc tgaactacat
    52381 ctcagttcgc ggctttggtg aagcggaata ctggttctca ctgatcaaag tcacgacagt
    52441 tattgtcttt atcatcgttg gcgtgctgat gattatcggt atcttcaaag gcgcgcagcc
    52501 tgcgggctgg agcaactgga caatcggcga agcgccgttt gctggtggtt ttgcggcgat
    52561 gatcggcgta gctatgattg tcggcttctc tttccaggga accgagctga tcggtattgc
    52621 tgcaggcgag tccgaggatc cggcgaaaaa cattccacgc gcggtacgtc aggtgttctg
    52681 gcgaatcctg ttgttctatg tgttcgcgat cctgattatc agcctgatta ttccgtacac
    52741 cgatccgagc ctgctgcgta acgatgttaa agacatcagc gtcagtccgt tcaccctggt
    52801 gttccagcac gcgggtctgc tctctgcggc ggcggtgatg aacgcagtta ttctgacggc
    52861 ggtgctgtca gcgggtaact ccggtatgta tgcgtctact cgtatgctgt acaccctggc
    52921 gtgtgacggt aaagcgccgc gcattttcgc taaactgtcg cgtggtggcg tgccgcgtaa
    52981 tgcgctgtat gcgacgacgg tgattgccgg tctgtgcttc ctgacctcca tgtttggcaa
    53041 ccagacggta tacctgtggc tgctgaacac ctccgggatg accggtttta tcgcctggct
    53101 ggggattgcc attagccact atcgcttccg tcgcggttac gtattgcagg gacacgacat
    53161 taacgatctg ccgtaccgtt caggtttctt cccactgggg ccgatcttcg cattcattct
    53221 gtgtctgatt atcactttgg gccagaacta cgaagcgttc ctgaaagata ccattgactg
    53281 gggcggcgta gcggcaacgt atattggtat cccgctgttc ctgattattt ggttcggcta
    53341 caagctgatt aaaggaactc acttcgtacg ttacagcgaa atgaagttcc cgcagaacga
    53401 taagaaataa gtttcctccc ttccttgcta agccctctca accgagaggg ctttttcaat
    53461 tccatttccc tgacaaatca tgcggatata aaatttaaca tttggattga taattgttat
    53521 cgtttgcatt atcgttacgc cgcaatcaaa aaaggctgac aaatcagagg ctggtctggc
    53581 tttctgggat gatcacctgc atacaaataa gtccaccgcg atgctgccat acgcaagggg
    53641 acgtgaagaa gatgtgagcg ataacccatt ttattttcgt agttacctca tggagatatg
    53701 gaatgtttag gttgaaccct ttcgtacggg tcgggctgtg tttgtccgct atttcttgtg
    53761 catggcctgt gttagcggtc gatgatgatg gcgaaacgat ggttgtcact gcatcttccg
    53821 ttgagcaaaa ccttaaagat gcacccgcca gtatcagcgt cattacccag gaagacctgc
    53881 agcgaaaacc ggtacagaat ctgaaggatg tcctcaaaga agtgcctggc gtacaactga
    53941 cgaacgaagg ggataaccgt aagggcgtta gtattcgtgg tctggacagc agctacaccc
    54001 tgattctcgt cgacggtaaa cgcgttaact cccgcaatgc cgtcttccgc cacaatgatt
    54061 tcgatctgaa ctggatcccg gtcgattcca tcgaacgtat tgaagtggtc cgtggcccga
    54121 tgtcgtcgct gtacggttcc gatgcgctcg gcggtgtagt gaatatcatc accaaaaaaa
    54181 tcggtcagaa atggtcgggt accgttaccg tcgataccac cattcaggaa catcgcgatc
    54241 gcggtgatac ctataacggt cagttcttta ccagcggacc attaattgat ggtgtgctgg
    54301 gaatgaaagc ttacggcagc ctggcaaaac gtgaaaagga tgacccgcaa aactcaacga
    54361 ccaccgatac cggagaaacg ccgcgtattg aaggattctc cagccgcgac ggcaatgtgg
    54421 aatttgcctg gacaccgaat caaaatcacg attttactgc cggatacggt ttccgaccgt
    54481 caggatcgtg attccgactc gctggacaaa aaccgcctgg aacgccagaa ctactccgtc
    54541 agccataatg ggcgttggga ttacggcacc agcgaactga aatactacgg tgagaaagtc
    54601 gagaacaaaa accctggcaa cagcagcccg ataacttccg aaagcaatac ggtcgacggc
    54661 aaatacacgt tgccgctgac ggcgattaat cagtttctca cggttggcgg tgaatggcgt
    54721 cacgacaaac ttagcgatgc ggtgaacctg actgggggaa ccagctccaa aacatctgca
    54781 agccagtacg cgctatttgt tgaagatgaa tggcggatct tcgagccgct ggcgctgacg
    54841 accggcgtac gtatggacga tcatgaaacc tacggtgaac actggagtcc gcgtgcctac
    54901 ctggtttata acgccaccga caccgtaacg gtgaaagggg gctgggcgac ggcatttaaa
    54961 gcaccttctc tgttgcaact tagccctgac tggacgagca attcctgccg tggcgcatgt
    55021 aagattgtgg gtagcccgga tctgaaacca gaaaccagcg aaagttggga gctggggctt
    55081 tactacatgg gcgaagaagg ctggctggaa ggggttgaat ccagcgttac cgttttccgt
    55141 aacgatgtga aagatcgtat cagcatcagc cgtacgtctg acgtcaatgc tgcaccaggc
    55201 taccaaaact ttgttggttt tacagcgaac ggtgtgccgg tatttagcta ctacaacgtt
    55261 aacaaagcac gtattcaggg cgtggaaacc gagctgaaaa ttccgttcaa cgatgaatgg
    55321 aaactgtcac tcaactacac ctacaacgat ggtcgtgatg tcagcaacgg cgaaaataaa
    55381 cctttatccg atctgccgtt ccataccgct aacggtacgc tggactggaa accgctggcg
    55441 ctggaagact ggtcattcta tgtttccgga cactataccg ggcagaaacg cgccgacagc
    55501 gcgacggcta aaacaccggg cggttatacc atctggaata ccggtgcggc ctggcaggtg
    55561 actaaagacg tcaaactgcg cgcaggcgtg ctgaaccttg gcgacaagga tctcagtcgt
    55621 gacgactaca gctataacga agacggacgt cgttacttta tggcagtgga ttatcgcttc
    55681 tgatgagaag atgcccggcg aaccgggcgg actttcactt cagtaaatac tgcgcatgga
    55741 agcgcaggtg atcctctata aaagaggcga tgaagtagta actgtgatca tatcccggct
    55801 gaatacgcag cgtcatcggc cacgctttct ggcgggcggc ttctgccagc actgcgggtt
    55861 gcagctggtc ggcaagaaac tgatcattat caccctgatc gataagcgtc gggatcgcat
    55921 cctgcgcgtt actggcatac atcagcgcac aactgtccca ttccagccat gcatttttgt
    55981 cctcacctaa atagctgcta aacgctttaa tgccccaggg tacgctgcat ggattcacaa
    56041 tcggcgcaaa ggcggaaacg ctggtgtatt tgcccgggtt tttcagcgcc ataatcagcg
    56101 caccgtgacc acccattgaa tgaccgctaa tagcgcagcg gtcgctgaca ttaaattgcg
    56161 actgaaccag cgccggtaat tcatcgcgca gataatcata catccggtaa tgcgtcgccc
    56221 acggcggttg cgtggcatta agataaaagc ctgcgccctg gcctaaatcg tagccatcgt
    56281 cgttggcaac ctgttcgccg cgcgggctgg tgtctggcat caccagtaca atccccagtt
    56341 ctgccgccac gcgctgggca cccgccttgg tggtgaagtt ctcgtcattg caggttaatc
    56401 cggaaagcca gtacagcact ggtggcgggg tgtgatcacg aggtggtggg agaaagatac
    56461 tgaacgtcat cggacagttt aaggtactgg agtcgtgtcg ccagcgttgc tgccagcctt
    56521 caaaacagcg gtgctcttcg agcatttcca tgcgtggctc cttgttgtgt tgtttgcaat
    56581 ttcttcatca taccgtttat tcaatgtgct gtgagtaact ttcacttccg tatttgcata
    56641 acgatgtttt aacatctgct gatgaaaggc agcggcaatt acaataatta tcgctgtgaa
    56701 tactggatta tgtgcgccgc ctcacgcaca ataatcaggc tgtaaatcag cttaataact
    56761 ttgcccccac gcagggcgga ggcgtcacac ctgcaggaga aatcataaat gccatcactc
    56821 agtaaagaag cggccctggt tcatgaagcg ttagttgcgc gaggactgga aacaccgctg
    56881 cgcccgcccg tgcatgaaat ggataacgaa acgcgcaaaa gccttattgc tggtcatatg
    56941 accgaaatca tgcagctgct gaatctcgac ctggctgatg acagtttgat ggaaacgccg
    57001 catcgcatcg ctaaaatgta tgtcgatgaa attttctccg gtctggatta cgccaatttc
    57061 ccgaaaatca ccctcattga aaacaaaatg aaggtcgatg aaatggtcac cgtgcgcgat
    57121 atcactctga ccagtacctg tgaacaccat tttgttacca tcgatggcaa agcgacggtg
    57181 gcttatatcc cgaaagattc ggtgatcggc ctgtcaaaaa ttaaccgtat cgtgcagttc
    57241 tttgcccagc gtccgcaggt acaggaacgt ctgacgcagc aaattctcat tgcgctacaa
    57301 acgctgctgg gcaccaataa tgtggctgtc tctatcgacg cggtgcatta ctgcgtgaag
    57361 gctcgtggca tccgcgatgc aaccagtgcc acgacaacga cctctcttgg tggattgttc
    57421 aaatccagtc agaatacgcg ccacgagttt ctgcgcgctg tgcgtcatca caactgataa
    57481 aaaggcagga accgtggagc gcaacgtcac gctcgatttt gttcgcggcg tcgccattct
    57541 ggggatcctg ctattaaaca tcagcgcctt tgggctacca aaggcggctt atctcaatcc
    57601 cgcctggtac ggcgctatta cgccacagga tgcatggacc tgggcatttc tcgatctcat
    57661 cggccaggtg aaattcctca cgctttttgc gctgctgttt ggtgctggcc tgcaaatgtt
    57721 gctgccccgt ggcagacgct ggatccagtc gcggttaacg ctgttagtct tgctgggctt
    57781 tattcacggt ttattgttct gggacggcga tattctgctg gcttacgggc tggtgggctt
    57841 aatctgctgg cggctggtgc gcgatgcgcc atcggtaaaa agcctgttta ataccggcgt
    57901 catgctttat ctggtgggtc ttggcgtttt gctgttattg gggttgattt ccgatagcca
    57961 gaccagccgc gcctggacac ctgatgcatc ggctattttg tatgaaaagt actggaaact
    58021 tcacggcggc gttgaagcga tcggtaatcg tgccgatggt gttggcaaca gtttactggc
    58081 actgggcgca cagtatggct ggcaactggc agggatgatg ctcattggtg cggcgctgat
    58141 gcgcagcggc tggctgaaag ggcagttcag cttacgtcac tatcgtcgta ctggttttgt
    58201 gctggtggcg attggggtga ccattaacct tcctgccatc gccctgcaat ggcagctgga
    58261 ctgggcgtat cgctggtgtg ccttcttact tcaaatgccg cgggaactga gtgcgccgtt
    58321 tcaggcgatt ggctatgcgt cgctgtttta tggcttctgg ccgcaattaa gccgctttaa
    58381 gctggtgctg gcaatcgcct gtgtcggacg gatggcactg accaactatc tattgcaaac
    58441 gctgatttgt actacgcttt tttaccacct cggtttgttt atgcagtttg accgtctgga
    58501 gctgctggcg tttgttattc cggtatggct ggcgaatatc ttcttctctg ttatctggct
    58561 gcgttacttc cgccaggggc cggtggaatg gctctggcgt cagttaactt tgcgtgctgc
    58621 cggaccgaca atatctaaaa catcaagata acgatctgga tcacattcgt taacaaaacg
    58681 gctgtaaccg tttccattgc tgtgactcga ttcacgaagt cctgtattca gtgctgacaa
    58741 aatagccgcc agcaagcagt catttactgc aatctcataa caggtagtga atatgatcac
    58801 cattcgtgat gtagcgcgtc aggctggcgt ctctgtggca acggtttccc gagtgctcaa
    58861 taacagcacg ctggtcagtg ccgacacgcg tgaagcagta atgaaagccg ttagtgagct
    58921 ggattatcga ccaaatgcca atgcccaggc gctggcaact caggttagcg acaccattgg
    58981 cgtggtggtg atggacgttt ctgatgcgtt tttcggcgcg ctggtaaaag cggtggatct
    59041 ggtcgcccag cagcatcaga aatacgtgct aatcggcaat agctatcatg aagcggaaaa
    59101 agagcgtcac gccattgagg tgttaattcg ccagcgttgt aatgcattga ttgttcactc
    59161 aaaagcattg agtgacgatg aactggcaca atttatggat aacattcccg gtatggtgtt
    59221 aatcaaccgc gttgtgccgg ggtacgccca tcgttgcgtt tgcctggata atctcagcgg
    59281 tgcccgaatg gcgacgcgca tgttgctgaa taacggtcat caacgtattg gttatctttc
    59341 ttccagccac ggcattgaag atgacgccat gcgtaaagca ggctggatga gtgcgttgaa
    59401 agagcaggat attattccgc cggaaagttg gattggcact ggcacgcctg acatgccggg
    59461 cggtgaggcg gcgatggttg aactgctggg gcgcaatcta caacttaccg ctgtatttgc
    59521 ttataacgac aatatggccg ctggcgcact gacagcatta aaagataatg gcattgcgat
    59581 tccgttacat ctctccatca tcggtttcga tgatattccc atcgcccgtt acaccgaccc
    59641 gcaattaacg accgtacgtt atcccattgc ttcaatggcg aaattagcca ccgaactggc
    59701 cttgcagggg gcagcaggca atattgatcc tcgtgccagc cactgtttta tgccgacgtt
    59761 agtgcgtcgc cattctgtcg caacacgcca gaatgcggcg gcgatcacta actcaacaaa
    59821 tcaggcgatg taaccgcttt caatctgtga gtgatttcac agtatcttaa caatgcgata
    59881 gctatgattg caccgtttta acgttgtaac ccgtatgtaa cagtgaataa tcactttcac
    59941 cgaggtaata gcgtcataac aacaattaaa gccgttttct ggagcgttac cgggcatgga
    60001 agaacgaatt ttaaaaagtg agcttcggcg ttcagtaaca cttcattaac tctactgctc
    60061 cgccgagcat ttatctcaag cactaccctg cataagaaaa accggagata ccatgaataa
    60121 gaaggtgtta accctgtctg ctgtgatggc cagcatgtta ttcggtgccg ctgcacacgc
    60181 tgctgatact cgcattggtg taacaatcta caagtacgac gataacttta tgtctgtggt
    60241 gcgcaaggct attgagcaag atgcgaaagc cgcgccagat gttcagctgc tgatgaatga
    60301 ttctcagaat gaccagtcca agcagaacga tcagatcgac gtattgctgg cgaaaggggt
    60361 gaaggcactg gcaatcaacc tggttgaccc ggcagctgcg ggtacggtga ttgagaaagc
    60421 gcgtgggcaa aacgtgccgg tggttttttt taacaaagaa ccgtctcgta aggcgctgga
    60481 tagctacgac aaagcctact acgttggcac tgactccaaa gagtccggca ttattcaggg
    60541 cgatttgatt gctaaacact gggcggcgaa tcaggggtgg gatctgaaca aagacggtca
    60601 gattcagttc gtactgctga aaggtgaacc gggccatccg gatgcagaag cacgtaccac
    60661 ttacgtgatt aaagaattga acgacaaagg catcaaaact gaacagttac agttagatac
    60721 cgctatgtgg gacaccgctc aggcgaaaga taagatggac gcctggctgt ctggcccgaa
    60781 cgccaacaaa atcgaagtgg ttatcgccaa caacgatgcg atggcaatgg gcgcggtaga
    60841 agcactgaaa gcacacaaca agtccagcat tccggtgttt ggcgtcgatg cgctgccaga
    60901 agcgctggcg ctggtgaaat ccggtgcact ggcgggcacc gtactgaacg atgctaacaa
    60961 ccaggcgaaa gcgacctttg atctggcgaa aaacttggcc gatggtaaag gtgcggctga
    61021 tggcaccaac tggaaaatcg acaacaaagt ggtccgcgta ccttatgttg gcgtagataa
    61081 agacaacctg gctgaattca gcaagaaata agactgattg ggcgcagtcg tttgctgcgc
    61141 ccttattgta tggccaacaa ggtaaaatta tatggtcagc tcaacgactc cgtcttccgg
    61201 agaatacttg ttggaaatga gcggtatcaa caagtctttt cctggtgtta aggcacttga
    61261 taacgttaat ttaaaagtcc ggccacattc tatccatgca ttaatggggg aaaacggcgc
    61321 aggaaaatca acattattaa aatgcctgtt tggtatttat caaaaagact ccggcaccat
    61381 tttattccag ggtaaagaga tcgatttcca ttctgccaaa gaagcgctgg aaaatggtat
    61441 ttcgatggta caccaggagt taaacctggt attacaacgt tcggtgatgg ataacatgtg
    61501 gctggggcga tatcccacca aaggcatgtt tgtcgatcag gacaaaatgt accgcgaaac
    61561 caaagcgatt tttgatgaac tggatattga tatcgatccg cgtgcgcgcg tcggcacatt
    61621 atccgtttcg caaatgcaga tgatcgaaat cgccaaagcg ttttcctata acgcgaaaat
    61681 tgtgattatg gatgaaccga cttcttcgtt aaccgaaaaa gaggtcaatc atctgttcac
    61741 tattattcgt aaattaaaag agcgcggctg cggtattgtt tatatctcac ataaaatgga
    61801 agaaatcttc cagttatgtg atgaagttac cgtattgcgc gacggtcagt ggatcgccac
    61861 tgaaccgctg gcaggactga cgatggacaa gatcatcgcc atgatggttg ggcgttctct
    61921 taaccagcgt ttccctgaca aagaaaacaa gccgggcgaa gtgattcttg aagtgcgtaa
    61981 cctgacgtcg ctgcgccagc cgtcgattcg cgatgtctcg tttgatctgc ataaagggga
    62041 gatcctcggt attgccggtc tggtgggggc gaaacgtacc gatattgttg agacgttatt
    62101 tggtattcgc gagaaatcgg ctggcaccat tacgttgcac ggcaaaaaga tcaataacca
    62161 taatgccaac gaagccataa accacggatt tgcactggta actgaagagc gccgctcaac
    62221 gggaatttat gcctatctgg atatcggttt taactcgtta atttccaata ttcgcaacta
    62281 caaaaataaa gttggtttac tggataactc gcggatgaaa agcgataccc agtgggtgat
    62341 tgattcgatg cgggtaaaaa caccggggca tcggacgcaa attggttcgc tctccggcgg
    62401 taatcagcaa aaggtgatta ttggtcgctg gctactaacg caaccagaaa tattaatgct
    62461 cgatgaaccg acgcgcggta ttgatgtcgg agcgaagttt gagatctatc agttaattgc
    62521 cgaactggcg aagaaaggca aagggattat tattatctcc tctgaaatgc ctgagttgtt
    62581 agggataaca gaccgtattc tggtcatgag caatggtctc gtttccggaa ttgtcgatac
    62641 aaaaacaaca acgcaaaacg aaattctgcg tcttgcgtct ttgcaccttt aagattaggg
    62701 gcttcccatg agtgcgttaa ataagaaaag ttttcttact tacctgaaag agggcggtat
    62761 ttacgtcgtt cttttagttt tgctggcaat tattattttc caggacccaa catttttaag
    62821 tctgttgaac ttaagtaata ttctcaccca gtcatcggtg cgtattatta tcgcgctcgg
    62881 tgtggcaggg ttaattgtca cccaggggac cgacctttct gcaggtcgcc aggtggggct
    62941 ggcggcagta gtagcggcga cgttattgca gtccatggat aacgccaaca aagtgttccc
    63001 ggaaatggcg acgatgccga ttgcgctggt tattctgatt gtctgtgcca ttggtgcggt
    63061 gatcggtttg atcaacggtc tgattatcgc ctatctcaac gtgacgccgt tcattaccac
    63121 gctcggcacg atgatcatcg tctatggcat caactcgctc tattacgact ttgtcggggc
    63181 gtcgccgatt tctggtttcg acagtggctt ctctaccttt gctcagggct ttgtcgcgct
    63241 ggggagtttc cgtctctctt acatcacctt ctacgcgttg attgcggtgg cgttcgtctg
    63301 ggtgttgtgg aacaaaaccc gcttcggtaa gaacattttt gccattggcg gtaacccgga
    63361 agcggcgaaa gtatctggtg tcaacgtcgg cctgaacctg ctgatgatct acgcgttgtc
    63421 tggcgtgttc tatgccttcg gcgggatgtt agaagccgga cgtatcggct ctgccaccaa
    63481 caacctcggc tttatgtatg aactggatgc tatcgcggcg tgcgtggtgg gcggcgtatc
    63541 gttcagcggc ggtgtgggga cggtgattgg cgtggtgacc ggggtgatta tttttaccgt
    63601 catcaactat ggcctgacgt acatcggcgt aaacccatac tggcagtaca tcatcaaagg
    63661 ggcgattatt atcttcgccg tggcgctgga ttcactgaaa tacgcacgta agaagtgatg
    63721 tgattgtggg tattgccgga tgcgccgcat ccggcaatcg gtgccaaatg ccggatgcaa
    63781 cgctggcgcg ttttatccgg cctacaatga gcgcaacata ctgatctata tgggtttata
    63841 tgccggatgc aacgcttgtg cgttttatcc ggcctacaat gagaacaaca tattgattta
    63901 tatggttttg taggccggat aaggcgttca cgccgcatcc ggcgacagga gccaaatgcc
    63961 ggatgcaacg ctggttttac aacgttaccg ggtgttcttt ctctcccttc ttaaacttca
    64021 cttctccgag atcaatacaa cccaccgggc agacgtgacc acaaagcagg caaccaacac
    64081 atttctcggt attacaatgc ggtgtacggg ttttctcgct ccattccatc gcctggtgac
    64141 cgccgtcgta acaggaaata taacagcgtc cacagccaac acatttatcg agattgatat
    64201 ggggatagac aatataactg cggtctaaat cttccgccgg gacaatattg ttattcgcca
    64261 gacctaccat atcctgcaac gaatcaaacc cttgatcggc gagataatgc gacaacccgc
    64321 tcgccatatc ttccactatc cgatacccgt actgcataat gccggtggtc acctgcaacg
    64381 tcgctgcacc gagcaataaa aactctgccg catcttccca ggtttcaata ccgccgatac
    64441 cgctgattgg gaaatcgcgc agctctggat gggtgcgcat ttgctgaata aagcgcaggg
    64501 cgatcggttt taccgcttta ccggaatatc cggaaatact cgatttaccg ttaacgatcg
    64561 gcataccgat tttctgatta agatcgatat tggtgatgga tttaacggtg ttaatcgccg
    64621 caatgccatc tgcaccgccg cgttttgccg ccagcgccac ttcgcacata tcaccgatat
    64681 tcggcgtcat cttcgccagc attggcagcg tggaaccacg ttttactgcc cgacaatatt
    64741 tttctaccag ctccgggctt tgcccgacat cgctacccat cgcatgagaa gtcatttgcg
    64801 gacaggagaa gttacactcg atcatatccg cgccagcttc ttgcaccaga cgcgccagct
    64861 cttcccattg ctgctcattt tcgcccatga ttgaagcgat caatactttg tccgggtaat
    64921 cttctttcag ccgacgcagt gcggccagat tatcttccaa cggatgttca gcaatctgct
    64981 ccatattttt gaagccgata aaaccggtat cttctttcac cagatgatca aaacgcggcg
    65041 agacttcgtt ggcgataaaa aaaccgatcg ttttaaacac cacgccaccc cagcctgtgt
    65101 cgtaggcttt ggcacacatc tcatagcagt tgcctaccgg cgaagaagag aggcagaacg
    65161 ggttgggaaa cttcacgccg caaaaggtaa tagaaagatc tttcgttaac atgagcaagc
    65221 tccctctaaa taatgatgaa tcgccccggc ggcttctttc ccggttttca ctgcatagac
    65281 cacggttttg tcaccctcaa caatatcgcc agcagcaaag acttgcggat cgcgggtctg
    65341 gtaattttgt gtattgatgg tgttacgctg cggctctaac tccgcaaagg catccagtct
    65401 ggcatgctga ccgacggcga gaataatttt atctgccgcc atcgtcagtt cgcccggtaa
    65461 ccgtacatgc ttaaacgtga ctttattgcc ttccacggct actggcgtga atccatcaat
    65521 gatcgaaacg cccagctccc gggcgctggc aaactctttt tcgcttgccg gaaactcatc
    65581 taactcttca cgcgctacgc aagttactgc ctgacagccg agaactttca gcgtgctggc
    65641 tacgtccatc gcgacatcac cgccgccgat aattaatgcg ctttgtggaa tgctgatatc
    65701 gccttgcgcc tgtcgtgcac gttgcaagaa gtcgacggca atctcaacgt cgctatgctc
    65761 aaacagtggt agccccgaac cgctcgataa ccccacagtg accagtaccg cgcggttttc
    65821 cgctttcagc tgctccaggg tgagtgtttt accgatttct ttattgcact taatggtcac
    65881 gcccattttt tcaatacggg cgatttctgc atccagtact gattgcggta accggaattg
    65941 cggaatgccg ttacgcaacc agccaccggc ttgtgcttct ttctcataga tcgtgacgtc
    66001 ataaccctgg tttgtcagtg tcacactggc ctgcaatccg gcaggacccg cgccaataat
    66061 ggcgactttg ccgagcgttt tagtaccggg ttgataaatt tccatcccag tttgttgttc
    66121 aaaatcagta acaaaacgct gtaagcgacc gatatcaatg ggagtatcaa caccggcacg
    66181 ggtacaaccg ctttggcata atttctccgt cgggcacact ctggcacaaa cggcaccgag
    66241 ggcattattt tcacgaattg tctcggcagc gcctttaaaa ttacgaaagt agattgagcg
    66301 aataaatttc cccggatcgg tctgtgccgg gcaagcctga ctacaggggg cgtcatggca
    66361 taataaacag cgagaggctt ctttaatcgc cagtaacggt gtaaaagccg gagtgagttc
    66421 atccagataa ttttgttgcg gcatgtgatc ttccttaatg ctatgagtaa ttaaggcgaa
    66481 cgctaaatat actcgtcata cttcaagttg catgtgctgc gtctgcgttc gctcacccca
    66541 gtcacttact tatgtaagct cttggggact cactcacttg tcgccttcct gcaactcgaa
    66601 ttatttagag tataaatatt gcgtgctgat attaaggcta tgcgcgtggg gaaatttaaa
    66661 ctgtgaaagg attcacaaat agctgcggtt cagaatgtta tatccgcagc ttattgagtg
    66721 aagtaataat tattctcaat aattaacaga aaagagaaaa tattaacgat agaaaataag
    66781 ataaacaatc atgccgatag ataaaattcc gaagccgata tatgccacca tataagcgaa
    66841 gaagagtccg ggattttttg ctcgccagac atacaccggt tccggtgcat ttttaacccg
    66901 tttatcaatc gcgcctgcgc gccagccttt ccatgcttcg cggaaacaaa ataccgggat
    66961 aaggaaaaaa acggcaacga caaaaaactg ctgtacatcc ataactactt tccttgtttc
    67021 ttttgtaatt caagtaactg ttcgggtgtg acggcgggat acccctgcgc atcttcaggc
    67081 acctggtgca tagccggaat agggaccagc ggccctaaaa aacgcggttc acgtttaaaa
    67141 atataaaggt cagccagcgc accgaaacgg gcggcaaatt cgcgaatacg tactgacagc
    67201 atatctttcg gtgacggtac ggcataacac tgagcctgaa tccccatatg cagcgcaata
    67261 aataatgccc gctcacagtg gaaacgttgg gtgataataa tgaaatcatt agtatcgaaa
    67321 actttgcgtg tacgcacgat ggaatccagc gtacgaaagc ctgcgtaatc gagaacaata
    67381 tctgatgggt cgacaccagc agcgattaaa tctttgcgca tggtcatcgg ctcattataa
    67441 ctttgcaatg cgttatcgcc gctcagtaat agataattta ccttaccgct gttataggca
    67501 ttaatcgctc cttgaatgcg gtagcgataa tactgattaa ttacgccagt ccgataatat
    67561 tttgctgtgc cgagcaccac accgacctgg cggtagggga gatcctgcaa ttcgtcgtag
    67621 atataaggcg cggttttcca gctcatccag cgatcgaggc cgagcacagt caacagcagc
    67681 aagccgatca ggactaacag gctgaggaac acgcgcttta acatgcagga gactcgatct
    67741 aaggaaaatt tccatcaggc tactttaccc gctggttttg cgcaagaaac ggcaaactgt
    67801 ttgccgggat tttcgacacc aggaaagggc tggcaagccg gacaaggcgt tcacgccgca
    67861 tccggcacca ggcttaagcg agaagcacgc ggtcgatatt gtgacagccg agagctttca
    67921 gcgttgcgga ggtggcatcc cactgaatca acggcgcatc ggctttttct gccagaaccg
    67981 cgcgctggat atccgggtaa tcataaccct tgagattcaa cagaattaac gccccctgca
    68041 acggcggcag agtcgggttg aatgcggcgt tttcagcgta gctgccgctg aaaatacgac
    68101 cgtctttaca ttccagcgcg acaccgcttg gcgacttact gtaaggcatg tgcgaacggt
    68161 ttgccgccgc aatcgccgcc tgagaaagcg catcaccagt cagcgcatag ccgtgatcct
    68221 gctcgtccat cagcagcgtt ttaatctcca gatctttcgg cccaaaggca tccggtagat
    68281 agtcacgcag cgcgtgtgcc tcgcgtcccg gaagatgaat acgcagatcc agaccgctgt
    68341 tcagttcatt cataaactga cggcagtgac cacaaggcgt gtagttaacg gtgatcgctg
    68401 caagcgcttt ttcaccactc agccaggcgt ggctgatcgc gctttgttcg gcatgaacgg
    68461 tttgctgcat tgtcgcacca ataaattcca tattggcacc gaaataccag gttccgctca
    68521 caccgcgcgc aattgcgcca acattaaaat tcgacaatgg cgtacgcgca caggctgccg
    68581 ccagcggaag tagtgcgaat gccagcgcgt cttcgtccag ccccgttgcg ctcttcagcg
    68641 atgagacttg ctccccggtc aacaaagcgg ggaagtactt gtctgccaga ataggttcca
    68701 gtgcagattg caagttatcc gcaagttggg caaaagcggt ttgaaaacgt ggatgcatgg
    68761 cgttgcctca taatcatgta atgggataac agtttacgaa cccgttatgg ctttatatgt
    68821 gatctgaatc tcattaatta tgcaacgcgt gaaacttaca tcaaaaatgc gcgacgcatc
    68881 gcaaatttta gcccattact gccagaataa tcgggaaaag gaacggtgcg atcagcgaag
    68941 taataatccc gcataacacc agcgccagcg aactaaatgc accttcctga taatccagct
    69001 cggcgcagcg cgccgtaccg agggcgtgcg aggctgtgcc cattgccaga ccgcgcgcag
    69061 ctttggtacg aatacgcatc gcattaagca atgtatggcc aaacacagcg ccgaggatgc
    69121 cgacgaaaat cacgcaaact gcgctgattg ccggaatacc gccaatactg ccgccaaccg
    69181 ccattgcaat aggcgtggtg actgatttcg gcaggattga cgcggcgatt tccggtgaag
    69241 cgcccatcaa caatgccaca gaagtccccg tcaccatcgc aaccacgctg ccgataaaac
    69301 agatggtgat gatcgatttc cagcgcgcgc ggatctggtg cagctgctca tataaaggat
    69361 aggccagtgc gacaaccgcc ggttgaagca gatcgtttag cacttcgcta cctttaaagt
    69421 agctgtcgta agagatgccg gtcagcatta aaaaaggaat gatcaccacc attgctacca
    69481 gcaacgggtt aagcagggga aacttatacc gtgccgccag tttgcgggcg gcaaaaaaga
    69541 caatcaacgt cagcggtaat gaccaccaga tattcgccat cattcttctg atcctttctg
    69601 acctacgact ttacgttcac cgtgcaccag ttgtgaactc cagctcacca ccagaaaaac
    69661 caccagcgta ctgactgcac aggaaaccac taccggacca aactgtgcgc ggagcaaatc
    69721 aaaatattgc atgacgccta caccaatcgg cacaaacaat agcgccatat agcgaatcag
    69781 tacgtagcac cccggattga cccatttcgc cggaagaatt tgcaaggcca gcaggacaaa
    69841 caggatcagc atcccgatga tgctgcccgg aatggttacc ggtagcaggg aagcaatgaa
    69901 aatgcctgca tacaggcagg cataaatcag gacaaaagca cgtaaatatt gccagataat
    69961 gttcagtgtc ttactcataa tcaacacccc agatgagatg gattcatcat acaattaatt
    70021 cgtaaaacgt gctaccgatc acattaagaa atctcagcat agttgaattt tagggacgta
    70081 acctcgggcg agcgcccagt tcagcacact aattctgctg taaaaagtgc agaaactgac
    70141 ttaaagcata agaaggcgtt tcgttattgc gccagaacac accgacgctg cctttctgtt
    70201 ctatctttgg caggttgaga atcaccagtt gtccctggct ggcataacgt tgcgccaggc
    70261 gtaatgacaa aatagagatc atatcgctgc tttgtaacag gttggtactg acgttcatcg
    70321 acgccgactc aatcgtgttt tctggcagca tcacgccgtg gtctaccagc gcattatcaa
    70381 tgctgatgcg gatgggggta cctgtcggcc agactatcca ccgccagtta gcaagatcat
    70441 tccagctgag ggtggcgtat ttcgccagtg gatgctcagg acgggcgaca aaacacaccg
    70501 gctcggtgta gagcacgcga taattcagcg gcagctgcaa cgcgcgaccg ccaattcgcc
    70561 caaccaccac atcgacagaa cctgccaaca gatcttgcag caatggggtc atcaccttct
    70621 cttcgatatt caggtgtaga gttggcatct ccttgagcaa atgcaaaatc gcctgggata
    70681 cgcagtcagt ggcgacggga gaacagccaa ttttcagact gccgaccagt ccaccttgtt
    70741 taaaacgggc cagttcatgt tgcgagcgct ccatgtcatt gattaaccgt tgggcatgtt
    70801 gcaataacaa tttgccgcct tcagtaggac gaagtccttt gctgtgccgc tcaaagagtg
    70861 tgaggccgat ctcatcttcg agttgtgaca accatttcga cagcgcaggc tgggtgatat
    70921 tcatcatccg cgcgacatgg gtcagattgc cctgttcacc cagcgccacc agcatttgca
    70981 ggtgatgaag ctttagtttc tgcgcccagt ttgccatttg ccataacctc caggttatct
    71041 ctctgtccga aatgccattg tacatttcat ctctttaatc acaatatcgc aacatacata
    71101 aaacaaatga aacatctacc ttacctgcac tgaggcattt actatgactc aacgacgtga
    71161 tttacaagca ctgatcgatg ctgctccggt cggcaagatg cagtggcgcg ttatcatctg
    71221 ctgttttctg gtggtaatgc tcgatggttt tgacaccgcg gctatcggct ttatcgcccc
    71281 tgatattcgc actcactggc aactcaccgc aggcgatctc gccccgcttt ttggcgcggg
    71341 tctgctgggg ttaaccgcag gcgcactgct ttgtggtccg ttgtccgatc gctttggccg
    71401 caaacgggtg attgagctgt gcgtcttttt gtttggcgcg ttaagcctcg cctcggcttt
    71461 ctcaccggat ctgcaaacgc tggtattcct gcgtttcctc accggccttg ggctgggtgg
    71521 cgcgatgcca aatacaatta ccatgacctc ggaatatctg cctgcccgcc gccgcggcgc
    71581 gctggtgacg ctgatgtttt gcggctttac cctgggttcg gcgtttggcg ggattgtcag
    71641 cgcgcagctg gtgccggtga ttggctggca cgggatcctg gtgttgggtg gcgtattacc
    71701 gctgatgctg tttgttgcac tgctggtggt tctgccggaa tcgccacgct ggcaggtgcg
    71761 ccgccagcta ccccaggcgg tgattgcgaa aaccgtcagc gccatcatcc gcgagcgtta
    71821 tgtcgatacc cacttttatc tcattgaatc agcctccgtt acaaaaggca gcattcgtca
    71881 actgtttatg gggcgtcagt tacccatcac gttgatgctg tgggtggtgt tctttatgag
    71941 cttgctgatt atttacctgc tctcaagctg gatgccaacg ctgctcaacc atcgcggcat
    72001 cgacctgcaa catgcctcct gggttaccgc cgcgttccag attggcggta cgttgggcgc
    72061 gctggcgctg ggcgtgctta tggataagtt caatcctttc cgcgtactgg cgctgagcta
    72121 tgccataggc gctgtatgca tcgtgatgat tggcttaagc caggacggat tatggctaat
    72181 ggcgctggcg attttcggca ccggcattgg catcagcgga tcgcaggtgg ggctgaatgc
    72241 gctgaccgca acactttatc caacacaaag tcgcgccacg ggcgtgagct ggtcaaacgc
    72301 cgtgggccgc tgcggcgcga ttgttggctc gctgtctggc ggcgttatga tggcgatgaa
    72361 tttctctttc gacaccctgt ttttcattat cgctgtgccc gcggcgatca gtgcggtgat
    72421 gttaacccag ctgatcaccg tggttcgcca gtcgacctct gtccctgact cactgcctca
    72481 cgcaggtgtt gtgaacgaat aaggagcatc ccaatgactg ataacaatca aaatagccgc
    72541 gagcaatttt atcagcatat ttccgggcaa aacctgaccc cgctgtggga gtcactgcac
    72601 catctggtgc caaaaacgcc caacgctaac tgtgcgccag cgtactggaa ttatcaggag
    72661 atccgcccgt tgctgctgga gagcggcagc ctgattggcg cgaaagaagc cgtgcgccgc
    72721 gtgctggtgc tggaaaaccc ggcgctgcgc gggcagtctt ccatcaccgc gactttatat
    72781 gccggtttgc aactgatcat gccgggcgaa gtggcaccga gtcatcgcca taaccagtcg
    72841 gcgctgcgtt ttattgttga aggtaaaggg gcattcaccg ccgttgatgg cgaacgcacg
    72901 tcaatgaatg agggcgattt tatcctgacc ccgcagtggc gctggcacga tcacggtaac
    72961 cctggcgacg aaccggttat ctggctcgat gggctggatc tgccattagt gaacactctg
    73021 ggctgcggtt ttgccgaaga ttacccggag gagcaacaac cagtaaggcg taaagagggc
    73081 gattacctgc cgcgttacgc tgccaatatg ttgccgttgc gccatcagac cgggaactcc
    73141 tcgcccatct ttaactaccg ttatgaccgt agccgcgaag tgctacacga tttaacccgc
    73201 ctgggcgatg ccgatgagtg ggatggctac aaaatgcgct acgtcaaccc ggtcaccggt
    73261 ggctatccga tgccgtcgat gggcgctttc ctgcaactgc tgccgaaagg gtttgcctca
    73321 cgcgtggcgc gtaccactga cagcaccatc taccatgtgg tggaaggtag cgggcaggtc
    73381 actatcggca atgaaacctt cagctttagt gcaaaagata tcttcgtggt gccgacctgg
    73441 cacggcgtgt cgttccagac cacgcaagat accgttttat ttagtttttc ggacagaccg
    73501 gtacaagaag ccctggggct gttccgcgaa gcacgttatt aatttgggag agagaaaatg
    73561 actcaatacg tattcgaacc acaagcgccc gttaccgtac cggttgttgg cagtgatgaa
    73621 caattccccg tacgccgcgt ttactgcgtc ggacgtaact atgccgccca cgcccgcgaa
    73681 atgggctttg atccggatcg tgaaccgcca ttcttcttct gcaaaccggc ggatgccatt
    73741 gttccggttg ccgctggcga aacgctggcg ctgccgtatc cggcgcagac cgataactac
    73801 cattatgaaa ttgaactggt ggtggcgatt ggtaaaaaag gcagtgatat cccgctggaa
    73861 aacgcccatg aatatgtctg gggttatgcc acgggtcttg atatgactcg ccgtgaccgt
    73921 caaatggaaa tgcgccagat gggccgcccg tgggaaatcg gcaaagcgtt cgatctctcg
    73981 gcccccatcg cgccgctgca caaagccagc gaaactggcg gtattgacaa cgcacctatc
    74041 tggttacagg ttaacggcga agatcatcag cgcagcgata ttcgtcatct catctggtca
    74101 gtgaatgaaa ccatcagcta cctgtcaggc ttctttgaac tgcaaccggg cgatctaatc
    74161 tttaccggta cgccggaagg ggttggctca gtagtcaaag gtgatgtgat cacgggcaac
    74221 gtcgagggct taacgcctat cgcggtgaag attgtctgag gtgatgatga agctgtacag
    74281 tttttttaat agttcagcgt cttaccgggt gcgcattgcg ctggcgctga agggtatcga
    74341 ttaccagacc gaaggggtga atatccgcat tggtcagcaa aacgaactgg cctaccggcg
    74401 gatgaatccg gtcgggctgg tgccgacgct gctgaccgac gagggcgaat cactcggaca
    74461 atcgctggcg attattgact ggctggagag tcactacccg caagttccgc tgatgccgca
    74521 ggaagaaccg gcccgcaaca aggtgctgga aattgtctat gccatcgcct gcgatattca
    74581 tccattgaat aacctacgcg tgctgcgcta cctgaccgaa gagctgaacg tcagtgaaga
    74641 agataaaaaa cgctggtatg cgcactggat ccagcaaggg ttaagcgcgg tggaacagct
    74701 actgcgccag agccagtcgg ggcaattttg tgtgggcgag acgccgacgc tggcagattg
    74761 ctgccttgtt ccacagtggg cgaacgcgct acgtatgaac tgcgatctga gcgggtatcc
    74821 gcgctgcaaa gcggtatacg acgcctgcac gcagctgcca gcgttcattg ccgcagcgcc
    74881 agaaaatcaa caagataaaa tttcagcctg accaggagaa ccataatgac taaagtgaca
    74941 cgcgcagtaa ttgtgggagg cgggatcggc tgtgcggcaa ctgcattgtc actggcccgc
    75001 caggggatca aagtggtgct gctggaaaag gcgcatgaga tcggtgagat tggcgcaggt
    75061 atccagttgg ggccaaatgc gttttcggcg ttggatagcc tcggcgtcgg cgaaatcgcc
    75121 cgccagcgcg cggtattcac cgatcacatc accatgatgg atgcggtaaa cgccgaagaa
    75181 gtggtgcgca ttgaaaccgg tcaggccttt cgcgatcatt tcggcggccc ttacgcggtg
    75241 atccaccggg ttgatattca cgcctcggtg tgggaagcgg tactgacgca tccggacgtg
    75301 gaatatcaca cctccaccaa cgtcgtggat attcgcgaga cagaagacga tgtcaccgtt
    75361 ttcgacgatc gcggcaatag ttggatcggc gatattctga ttggctgtga cggcgtgaag
    75421 tcagtggtgc gccagagctt attgggcgat gcgccgcgtg tcaccgggca tgtggtctac
    75481 cgtgctgtta tcgattgtgc cgatatgccg gaagatttgc gcattaacgc accagtactg
    75541 tgggcggggc cgtgctgtca cttggtgcat taccctttac gcggcggcaa gcagtataac
    75601 ctggtggtga cattccacag ccgccagcag gaagagtggg gcgtgaagga tggcagtaaa
    75661 gaggaagtgc tctcttactt tgaagggatt cacccccgtc cgcgccagat gctggataaa
    75721 ccgacctcct ggcgacgctg gtcaaccgcc gaccgtgaac cggtagcgaa gtggggaacg
    75781 aagcgcatta cgctggtggg cgatgcggcc catccggtgg cgcagtatat ggcgcagggt
    75841 gcctgtatgg cgctggaaga cgcggtaaca ctgggtaagg cgctggcgca gtgtgagggt
    75901 gatgcggcac aagcctttgc gctgtatgag tcggtacgta ttccgcgcac cgcgcgcatc
    75961 gtctggtcga cccgtgaaat ggggcgggtt tatcatgccg caggagtaga gcgccaggta
    76021 cgtaacctgt tgtggaaagg gaaatcgcag gcagagtttt accgcggcat ggagtggctg
    76081 tacggctgga aagaagataa ctgtttgcaa ccacgctgag tactttaatc cgctgaccat
    76141 aggcgctaac atagcgcctc atttttttgc gggtgatgat atgcgtgtgt tactggctcc
    76201 gatggagggg gtacttgact ctctggtgcg tgaattgctg accgaagtta acgactacga
    76261 tctgtgcatc accgagtttg tccgcgtggt ggatcaactg ctgccggtaa aagtctttca
    76321 tcgcatttgc cctgagctac aaaacgccag ccggacacca gccggtacgc tggtgcgcgt
    76381 gcagttgtta ggtcagttcc cacaatggct ggcagagaat gccgcccgtg cggtcgagtt
    76441 aggttcctgg ggcgtggatc tcaattgcgg ctgcccgtcg aaaacggtta acggtagcgg
    76501 cggtggggcg acgttactca aagatcctga actcatctat cagggtgcaa aagcgatgcg
    76561 cgaagctgta ccggcgcatt tgcccgtcag cgtaaaagtg cgtctgggct gggacagcgg
    76621 tgagaagaaa tttgaaatcg ccgatgcggt gcaacaggct ggcgctacgg agctggtagt
    76681 acatgggcgg acaaaagagc agggttaccg cgcggagcat attgactggc aggcgattgg
    76741 cgagattcgc cagcggttga atattcctgt gattgccaac ggtgaaatct gggactggca
    76801 gagtgcgcaa caatgcatga cgatcagcgg atgcgacgca gtgatgattg gtcgcggggc
    76861 gctcaatatt cccaacctga gccgggtggt aaaatataac gaaccgcgaa tgccgtggcc
    76921 ggaggtagtt gctttgctgc aaaaatatac ccgtctggaa aagcagggcg ataccgggtt
    76981 atatcacgta gcgcggatta aacagtggtt gagttatttg cgtaaagaat acgatgaagc
    77041 aacagaatta tttcagcatg ttcgggtgtt gaataattcc cctgatattg caagggctat
    77101 tcaggccatt gatatcgata agctgcgata atagtggaat cgcagcttat ctgagacaat
    77161 gaatcgctta ttcagcttca tcatcttgtt gcgctccttt catgagctaa gccacatatt
    77221 gccactggcg caaggagcgc gcagggggag gacaatcgcc tcctccccct gcacccccgg
    77281 gctctggcga acaaaatcgc cgctgcgcgg taccttcagc ttatcccctt cggctaccgg
    77341 gtcgggcgcg aggtaacgtc cttgtaagac gcgccctcag cccacatcca
//
LOCUS       DAPHCV010000006        76729 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.006, whole genome shotgun sequence.
ACCESSION   DAPHCV010000006 DAPHCV010000000
VERSION     DAPHCV010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 76729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.006"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            143..868
                     /locus_tag="RH033_000391"
     CDS             143..868
                     /locus_tag="RH033_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDV2989631.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            826..1503
                     /gene="bglJ"
                     /locus_tag="RH033_000392"
     CDS             826..1503
                     /gene="bglJ"
                     /locus_tag="RH033_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HDV2989632.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHIYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSKRREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1540..2328)
                     /gene="fhuF"
                     /locus_tag="RH033_000393"
     CDS             complement(1540..2328)
                     /gene="fhuF"
                     /locus_tag="RH033_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HDV2989633.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLRAMTLAQWSSPNTLSSQLAVYSDHIYRNQPLMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHVEFHETGRAACFWVDVCEDKNATLHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     MLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2469..2705
                     /locus_tag="RH033_000394"
     CDS             2469..2705
                     /locus_tag="RH033_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HDV2989634.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIIILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMIMNLKQG"
     gene            complement(2744..2830)
                     /locus_tag="RH033_000395"
     tRNA            complement(2744..2830)
                     /locus_tag="RH033_000395"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2794..2796),aa:Leu,seq:cag)
     gene            complement(3030..4061)
                     /gene="rsmC"
                     /locus_tag="RH033_000396"
     CDS             complement(3030..4061)
                     /gene="rsmC"
                     /locus_tag="RH033_000396"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HDV2989635.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLSDYAPLNKMDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            4164..4577
                     /gene="holD"
                     /locus_tag="RH033_000397"
     CDS             4164..4577
                     /gene="holD"
                     /locus_tag="RH033_000397"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HDV2989636.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPIVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            4546..4992
                     /gene="rimI"
                     /locus_tag="RH033_000398"
     CDS             4546..4992
                     /gene="rimI"
                     /locus_tag="RH033_000398"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDV2989637.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            5007..5684
                     /gene="yjjG"
                     /locus_tag="RH033_000399"
     CDS             5007..5684
                     /gene="yjjG"
                     /locus_tag="RH033_000399"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HDV2989638.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            5775..7364
                     /gene="prfC"
                     /locus_tag="RH033_000400"
     CDS             5775..7364
                     /gene="prfC"
                     /locus_tag="RH033_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDV2989639.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            7757..8362
                     /gene="osmY"
                     /locus_tag="RH033_000401"
     CDS             7757..8362
                     /gene="osmY"
                     /locus_tag="RH033_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HDV2989640.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESTGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSHAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            8489..8650
                     /locus_tag="RH033_000402"
     CDS             8489..8650
                     /locus_tag="RH033_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HDV2989641.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            8772..9845
                     /locus_tag="RH033_000403"
     CDS             8772..9845
                     /locus_tag="RH033_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HDV2989642.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYREIQQFIEKPPGQLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVMTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            9842..10621
                     /locus_tag="RH033_000404"
     CDS             9842..10621
                     /locus_tag="RH033_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDV2989643.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLKKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRTGVVHGFS
                     GSLQQAERFVLLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPEDEIAEVLLNNTYTLFNVP"
     gene            complement(10838..11701)
                     /locus_tag="RH033_000405"
     CDS             complement(10838..11701)
                     /locus_tag="RH033_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HDV2989644.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVNGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHIRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLCHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEHLAGALQERG
                     VSRLIFPALYL"
     gene            complement(11673..13223)
                     /locus_tag="RH033_000406"
     CDS             complement(11673..13223)
                     /locus_tag="RH033_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HDV2989645.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IVARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            13481..14260
                     /gene="deoC"
                     /locus_tag="RH033_000407"
     CDS             13481..14260
                     /gene="deoC"
                     /locus_tag="RH033_000407"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDV2989646.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            14387..15709
                     /gene="deoA"
                     /locus_tag="RH033_000408"
     CDS             14387..15709
                     /gene="deoA"
                     /locus_tag="RH033_000408"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HDV2989647.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            15761..16984
                     /gene="deoB"
                     /locus_tag="RH033_000409"
     CDS             15761..16984
                     /gene="deoB"
                     /locus_tag="RH033_000409"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDV2989648.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     HGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            17064..17783
                     /gene="deoD"
                     /locus_tag="RH033_000410"
     CDS             17064..17783
                     /gene="deoD"
                     /locus_tag="RH033_000410"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDV2989649.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(17944..18207)
                     /locus_tag="RH033_000411"
     CDS             complement(17944..18207)
                     /locus_tag="RH033_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV2989650.1"
                     /translation="MIDLENQEREIINLMFSQGISWLTAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAGIYGCPPELLICPSWMTAEHK"
     gene            complement(18239..19255)
                     /gene="lplA"
                     /locus_tag="RH033_000412"
     CDS             complement(18239..19255)
                     /gene="lplA"
                     /locus_tag="RH033_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HDV2989651.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQICEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSAWMA
                     GAVR"
     gene            complement(19283..19927)
                     /locus_tag="RH033_000413"
     CDS             complement(19283..19927)
                     /locus_tag="RH033_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HDV2989652.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTESPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVDVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            20033..21001
                     /gene="serB"
                     /locus_tag="RH033_000414"
     CDS             20033..21001
                     /gene="serB"
                     /locus_tag="RH033_000414"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HDV2989653.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGERVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPMMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            21050..22432
                     /gene="radA"
                     /locus_tag="RH033_000415"
     CDS             21050..22432
                     /gene="radA"
                     /locus_tag="RH033_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDV2989654.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            22453..23685
                     /gene="nadR"
                     /locus_tag="RH033_000416"
     CDS             22453..23685
                     /gene="nadR"
                     /locus_tag="RH033_000416"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HDV2989655.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            23777..24109
                     /locus_tag="RH033_000417"
     CDS             23777..24109
                     /locus_tag="RH033_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDV2989656.1"
                     /translation="MGKAIIFIETPMFTRQIKQIATDDELKELQKELIGTPDKGDLIQ
                     QTGGLRKVRMVAGSQGKSGSVRIIYFLATEEIIYLIMAYPKNAKDSLTDTEKAQLKKL
                     TKLLKGEI"
     gene            24111..24395
                     /locus_tag="RH033_000418"
     CDS             24111..24395
                     /locus_tag="RH033_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV2989657.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKEPARVTRYEIADVKAIREQLNV
                     SQSEMAKALGTSVDTIKSWESKRRNPTGLAAKVLATIKENPAFFRELASH"
     gene            complement(24451..26118)
                     /gene="ettA"
                     /locus_tag="RH033_000419"
     CDS             complement(24451..26118)
                     /gene="ettA"
                     /locus_tag="RH033_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HDV2989658.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVNNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            26454..28391
                     /gene="sltY"
                     /locus_tag="RH033_000420"
     CDS             26454..28391
                     /gene="sltY"
                     /locus_tag="RH033_000420"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HDV2989659.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            28481..28807
                     /gene="trpR"
                     /locus_tag="RH033_000421"
     CDS             28481..28807
                     /gene="trpR"
                     /locus_tag="RH033_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HDV2989660.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKDH"
     gene            complement(28801..29316)
                     /gene="yjjX"
                     /locus_tag="RH033_000422"
     CDS             complement(28801..29316)
                     /gene="yjjX"
                     /locus_tag="RH033_000422"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HDV2989661.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLHPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQ"
     gene            29368..30015
                     /gene="gpmB"
                     /locus_tag="RH033_000423"
     CDS             29368..30015
                     /gene="gpmB"
                     /locus_tag="RH033_000423"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HDV2989662.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLETRNIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            complement(30012..30881)
                     /gene="robA"
                     /locus_tag="RH033_000424"
     CDS             complement(30012..30881)
                     /gene="robA"
                     /locus_tag="RH033_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HDV2989663.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            31092..31565
                     /gene="creA"
                     /locus_tag="RH033_000425"
     CDS             31092..31565
                     /gene="creA"
                     /locus_tag="RH033_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HDV2989664.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            31578..32267
                     /gene="creB"
                     /locus_tag="RH033_000426"
     CDS             31578..32267
                     /gene="creB"
                     /locus_tag="RH033_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HDV2989665.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLALTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            32267..33691
                     /gene="creC"
                     /locus_tag="RH033_000427"
     CDS             32267..33691
                     /gene="creC"
                     /locus_tag="RH033_000427"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HDV2989666.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYIYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKKITLHVTPTEVNVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEYVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            33749..35101
                     /gene="creD"
                     /locus_tag="RH033_000428"
     CDS             33749..35101
                     /gene="creD"
                     /locus_tag="RH033_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HDV2989667.1"
                     /translation="MLKSPLFWKMTSLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPGGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGAIMNGIYLQAVLKGWCNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKDVTMDDELRIWK
                     "
     gene            complement(35160..35876)
                     /gene="arcA"
                     /locus_tag="RH033_000429"
     CDS             complement(35160..35876)
                     /gene="arcA"
                     /locus_tag="RH033_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HDV2989668.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            35972..36112
                     /locus_tag="RH033_000430"
     CDS             35972..36112
                     /locus_tag="RH033_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2989669.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            36512..37198
                     /locus_tag="RH033_000431"
     CDS             36512..37198
                     /locus_tag="RH033_000431"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HDV2989670.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAA
                     RWVAHGSGDIIDNIKVFKTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERAMVLLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            37412..37477
                     /gene="thrL"
                     /locus_tag="RH033_000432"
     CDS             37412..37477
                     /gene="thrL"
                     /locus_tag="RH033_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HDV2989671.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     misc_feature    37413..37532
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            37558..40020
                     /gene="thrA"
                     /locus_tag="RH033_000433"
     CDS             37558..40020
                     /gene="thrA"
                     /locus_tag="RH033_000433"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HDV2989672.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPTEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     gene            40022..40954
                     /gene="thrB"
                     /locus_tag="RH033_000434"
     CDS             40022..40954
                     /gene="thrB"
                     /locus_tag="RH033_000434"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDV2989673.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            40955..42241
                     /gene="thrC"
                     /locus_tag="RH033_000435"
     CDS             40955..42241
                     /gene="thrC"
                     /locus_tag="RH033_000435"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDV2989674.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGVGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            42455..42751
                     /locus_tag="RH033_000436"
     CDS             42455..42751
                     /locus_tag="RH033_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HDV2989675.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(42904..43680)
                     /gene="yaaA"
                     /locus_tag="RH033_000437"
     CDS             complement(42904..43680)
                     /gene="yaaA"
                     /locus_tag="RH033_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDV2989676.1"
                     /translation="MLILISPAKTLDYQSPLPTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(43750..45180)
                     /locus_tag="RH033_000438"
     CDS             complement(43750..45180)
                     /locus_tag="RH033_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDV2989677.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFALFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLYGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDISRQLSPDAWDDVSQE"
     gene            45459..46412
                     /gene="tal"
                     /locus_tag="RH033_000439"
     CDS             45459..46412
                     /gene="tal"
                     /locus_tag="RH033_000439"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDV2989678.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAVDQEKLEKMIGDLL"
     gene            46527..47114
                     /gene="mog"
                     /locus_tag="RH033_000440"
     CDS             46527..47114
                     /gene="mog"
                     /locus_tag="RH033_000440"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HDV2989679.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYIETAPEVVAAFRPKSARRDVSE"
     gene            complement(47149..47715)
                     /gene="satP"
                     /locus_tag="RH033_000441"
     CDS             complement(47149..47715)
                     /gene="satP"
                     /locus_tag="RH033_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HDV2989680.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(47864..48577)
                     /gene="msyB"
                     /locus_tag="RH033_000442"
     CDS             complement(47864..48577)
                     /gene="msyB"
                     /locus_tag="RH033_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HDV2989681.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGIAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(48603..49007)
                     /locus_tag="RH033_000443"
     CDS             complement(48603..49007)
                     /locus_tag="RH033_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HDV2989682.1"
                     /translation="MKSVFTLSASLAISLLLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQTLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            49383..51299
                     /gene="dnaK"
                     /locus_tag="RH033_000444"
     CDS             49383..51299
                     /gene="dnaK"
                     /locus_tag="RH033_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDV2989683.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            51388..52518
                     /gene="dnaJ"
                     /locus_tag="RH033_000445"
     CDS             51388..52518
                     /gene="dnaJ"
                     /locus_tag="RH033_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDV2989684.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(52622..52774)
                     /gene="mokC"
                     /locus_tag="RH033_000446"
     CDS             complement(52622..52774)
                     /gene="mokC"
                     /locus_tag="RH033_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HDV2989685.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            53360..54526
                     /gene="nhaA"
                     /locus_tag="RH033_000447"
     CDS             53360..54526
                     /gene="nhaA"
                     /locus_tag="RH033_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HDV2989686.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNHADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            54586..55491
                     /gene="nhaR"
                     /locus_tag="RH033_000448"
     CDS             54586..55491
                     /gene="nhaR"
                     /locus_tag="RH033_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HDV2989687.1"
                     /translation="MSMSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRAL
                     EERLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVA
                     DALSKRLVSSVLDAAVVEDEQIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQE
                     GLFSMKIGECGVSFWCTNPLPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNV
                     EILGEFDDAALMKAFGATHNAIFVAPSLYANDFYNDDSVVEIGRVENVMEEYHAIFAE
                     RMIQHPAVQRICNTDYSALFTPASK"
     gene            complement(55587..55850)
                     /gene="rpsT"
                     /locus_tag="RH033_000449"
     CDS             complement(55587..55850)
                     /gene="rpsT"
                     /locus_tag="RH033_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDV2989688.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            55953..56171
                     /locus_tag="RH033_000450"
     CDS             55953..56171
                     /locus_tag="RH033_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HDV2989689.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSARKIAAGGQFLPMICAIAMK
                     SHFFLISALNRRLTLTAVQGILGRFSLF"
     gene            56179..57120
                     /gene="ribF"
                     /locus_tag="RH033_000451"
     CDS             56179..57120
                     /gene="ribF"
                     /locus_tag="RH033_000451"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDV2989690.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            57163..59979
                     /gene="ileS"
                     /locus_tag="RH033_000452"
     CDS             57163..59979
                     /gene="ileS"
                     /locus_tag="RH033_000452"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDV2989691.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIIKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKTKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVSDYTILGTVKGAELELLRFTHPFMDFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            59979..60473
                     /gene="lspA"
                     /locus_tag="RH033_000453"
     CDS             59979..60473
                     /gene="lspA"
                     /locus_tag="RH033_000453"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDV2989692.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            60561..61010
                     /gene="fkpB"
                     /locus_tag="RH033_000454"
     CDS             60561..61010
                     /gene="fkpB"
                     /locus_tag="RH033_000454"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDV2989693.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            61012..61962
                     /gene="ispH"
                     /locus_tag="RH033_000455"
                     /gene_synonym="lytB"
     CDS             61012..61962
                     /gene="ispH"
                     /locus_tag="RH033_000455"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDV2989694.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLSGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            62028..62942
                     /gene="rihC"
                     /locus_tag="RH033_000456"
     CDS             62028..62942
                     /gene="rihC"
                     /locus_tag="RH033_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HDV2989695.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLEIP
                     AFLAIRDALLRAPEPVTLVAIGPLTNIALLLSQCPECKQYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            63109..63930
                     /gene="dapB"
                     /locus_tag="RH033_000457"
     CDS             63109..63930
                     /gene="dapB"
                     /locus_tag="RH033_000457"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDV2989696.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            64387..65535
                     /gene="carA"
                     /locus_tag="RH033_000458"
     CDS             64387..65535
                     /gene="carA"
                     /locus_tag="RH033_000458"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDV2989697.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEPYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            65553..68774
                     /gene="carB"
                     /locus_tag="RH033_000459"
     CDS             65553..68774
                     /gene="carB"
                     /locus_tag="RH033_000459"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDV2989698.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLHAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTTIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            69035..69430
                     /gene="caiF"
                     /locus_tag="RH033_000460"
     CDS             69035..69430
                     /gene="caiF"
                     /locus_tag="RH033_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HDV2989699.1"
                     /translation="MCEGCVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(69550..70140)
                     /gene="caiE"
                     /locus_tag="RH033_000461"
     CDS             complement(69550..70140)
                     /gene="caiE"
                     /locus_tag="RH033_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HDV2989700.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFRGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(70146..70931)
                     /gene="caiD"
                     /locus_tag="RH033_000462"
     CDS             complement(70146..70931)
                     /gene="caiD"
                     /locus_tag="RH033_000462"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HDV2989701.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGVVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            complement(71040..72593)
                     /gene="caiC"
                     /locus_tag="RH033_000463"
     CDS             complement(71040..72593)
                     /gene="caiC"
                     /locus_tag="RH033_000463"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HDV2989702.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSSGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREEST
                     WILQNSQACLLVTSAQFYPMYQQIQQEDASQLRHICLTDMALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQNYRATITECIPMMIRTL
                     MVQPLSANDRQHCLREVMFYLNLSEQEKDAFCERFSVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGELGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFCFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            complement(72666..73883)
                     /gene="caiB"
                     /locus_tag="RH033_000464"
     CDS             complement(72666..73883)
                     /gene="caiB"
                     /locus_tag="RH033_000464"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HDV2989703.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKARETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMTKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNVMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            complement(74001..75143)
                     /gene="caiA"
                     /locus_tag="RH033_000465"
     CDS             complement(74001..75143)
                     /gene="caiA"
                     /locus_tag="RH033_000465"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HDV2989704.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            complement(75174..76688)
                     /gene="caiT"
                     /locus_tag="RH033_000466"
     CDS             complement(75174..76688)
                     /gene="caiT"
                     /locus_tag="RH033_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HDV2989705.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILFWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
BASE COUNT        18506 a        19292 c        20649 g        18282 t
ORIGIN      
        1 ttgtgcagca ggagtatgct gatatgaaag tgaatgctaa ggataattta ttcacttaat
       61 ctattaattt gctgagaata tttaaggaat gaataccttg aataaaccat catgattttt
      121 agacagtgga tgtggaggaa atatgttgcc aggatgctgc aaaaatggaa ttgttatcag
      181 taaaatacct gttatgcaag cagggttaaa agaggtcatg aggactcact tccctgaata
      241 tgaaataata tccagcgcct ctgcggagga ccttacctta ttacaattac gtcgttccgg
      301 attagtcatt gctgatttgg ccggtgaaag tgaagatcca cgttccgttt gtgagcatta
      361 ttactcttta atctcacaat atcgggaaat tcactgggtt ttcatggtgt cacgctcctg
      421 gtattcccag gcagtagaat tgctcatgtg ccctacggcg acgttattgt ctgatgttga
      481 acccattgag aatctggtca agaccgtacg ttccggcaat acgcacgcag agcgtatcag
      541 cgccatgctg acctctccgg caatgactga aactcatgat tttagctatc gctcagtcat
      601 tctcactctt tcagagcgca aggtactgcg gctattaggt aaaggatggg gcatcaacca
      661 gatagcttca ttgcttaaga aaagtaataa aactatcagc gcccaaaaaa acagtgcgat
      721 gcgtcgactg gcaattcaca gtaacgctga aatgtatgca tggataaata gcgcgcaggg
      781 tgcaagagaa cttaacttgc cttctgttta tggagatgcc gcagaatgga acacagccga
      841 attaagaaga gaaatgtcgc actcatagaa aaatgcgtca tgagcagtat cggtattgag
      901 agtttattca gaaagtttgc aggtaaccct tataagctcc atatctatac cagtcaggag
      961 tcatttcagg atgccatgtc gcggatctcg tttgcggcgg tcattttttc tttttctgcc
     1021 atgagaagta aacgcagaga gggattatct tgcctgactg aactggcgat taagtttccg
     1081 cgtacccgac gtttagttat tgcggatgat gatattgaag ctcggctgat tggttcattg
     1141 tcgccatcac cgctggacgg tgtattaagt aaagcgtcga cgctggagat ttttcatcag
     1201 gaactctttt tgtcattaaa tggtgtgcgt caggcgaccg accgactgaa caatcagtgg
     1261 tacattaacc aaagccggac gctaagcccg acggagagag aaatattgcg ctttatgtcg
     1321 cgtggctact caatgacaca aattgccgag cagcttaaac gcaatatcaa aacgatccgc
     1381 gcgcataaat ttaatgtaat gtcgaaactg ggcgtcagtt ctgatgcagg gttgttggag
     1441 gccgcagata ttctgttatg tatgcggcat tgcgaagcaa gtaatgtgtt gcatccctat
     1501 taatccacat gatgccgggt ttacttcccc ggcagtgctt catttcagcg tacaatcgcc
     1561 acattgctgc acatccggta agcgataacg ctggcagcaa gtgcggcgca ccagcaggcc
     1621 gtcgcgcagt accacggtac gccagagtgg attatcttca ccgttcgtga gcattttctc
     1681 aaaaaagagg gcatggcgca gcgattcaac cgtcgcctcg ccaagcagct gtttcatctc
     1741 agtcagatac cagttgatca aataaccggt attgctccag ataagtttgc cgttgatctc
     1801 tcccgtcgct tctaatgcct gcactaccgg aacaagcgcc tggctgatta acgtttccat
     1861 tcgctgctgc ggcgaatgta gtgttgcgtt tttatcttca cacacatcga cccagaaaca
     1921 ggcggcgcgt ccggtttcgt gaaactcaac gtggaaatgt tccggcgaca catctaatgc
     1981 tttttcctgc gtcagcagcg ccaacattaa tggtggcacc atcaggccga tataccattg
     2041 tgcccatagt gagatcagcg gtttgttctc gcggatcatc aagggttggt tgcggtagat
     2101 atgatcggaa tagaccgcca gttgagaact tagcgtattc ggtgatgacc attgcgccag
     2161 cgtcatggca cggagcggtg cgggttcatc cagacggata aactccagca aatgttcacg
     2221 atgttcggcg atcgtcgccc gtacggcttg tgcaagccca gcatcctgcg gctggagatg
     2281 cgttcgccag ataacatctt catagagcgg tgcggaacga taggccataa tcgtgatagt
     2341 aatctaaatg ataatgattg ctaatcctag cgataggttt acccgatagc aagggatttc
     2401 tctggcttgc aaatgataaa aattatcata tgatattggt tatcattatc aatgaaagag
     2461 atgaaaccat gttgcaacgt acgctgggca gtggctgggg agtgttgctg ccgggattgc
     2521 tgattgcagg gctgatgtat gcggatttat cgccggatca atggcggatt atcattctga
     2581 tgggattagt attgacgccg gtgatgctgt atcacaaaca gttgcggcat tacgttttgc
     2641 tgccatcgtg cctggcgctt attgctggca tcatgctgat gataatgaat ttgaagcagg
     2701 gatgataaaa cagggaagaa acaagaaagg aagtaaagat aattggtgcg agggggggga
     2761 cttgaacccc cacgtccgta aggacactaa cacctgaagc tagcgcgtct accaattccg
     2821 ccaccttcgc ataccgtcaa ttcttaaaaa gaattgctac cacggaggcg cattctagtg
     2881 gttttcagct tttcgtcaat agttaattat cgacagaggt gtaattgctg gaaaaatgtc
     2941 catcagcgga ttgtggataa ttcgtttgtc ggtcagataa ggcgtgtacg ccgcatccga
     3001 cacggtatgc ggcgacatat atcagatact caacctttct tcgcctggcg ggtcataatg
     3061 gcgcgataca ccttgaagcg cccggtttgc gcgatcactt cgtggaagcc aaatgtctca
     3121 tccagcacgt ccggataagg caggaaggcg ttcgctacaa ttcgcagctc gccgccgcta
     3181 ttaagatgac gcaccgcgcc gcgaatcagc gtttgcgcgg catccaggct ggtttgcatc
     3241 ccatcgtgga acggcgggtt ggagatgatc atatcaaaac gacctttcac ctcggaaaag
     3301 acgttactgg caaagacttc accttcaaca ccgttggccg caagtgttgc gcggctggct
     3361 tctaccgctg gtgcagagac atcgcacaga gtgagacgaa ttttcggcga atggcgcgca
     3421 aaggcaaccg aaagtacgcc cgcgccacag ccgacatcca gcactttacc tttcgtgtgc
     3481 ggggttaacg tcgagagcag caactggcta ccgacatcca gaccgtcgcg gctaaacacg
     3541 ccaggcagcg ttttgaccgt caggccatcg acgctgtatt cgccccagaa tttatcggca
     3601 tcaaatactg gctgtttttc cagacgacca aaatagaggc cacagcgacg agcgctgtcc
     3661 attttattca acggcgcata atctgacagc atctgctcgg cgctgcgcac gccgctgcgg
     3721 ttctcgccaa cgacaaaaat atccgtgccc actggcagca gagaaagtaa attcatcaac
     3781 tggaactggg cttccggttt gttcttcggc cagtagtaaa tcagcgtatc gcaatctgcg
     3841 acatcatccg ccgtggcgac cagactaaaa cgggcgttat cccccatctg gcggcttaac
     3901 acctgccagt ggtggaattg ctgggtatga gcacggctgg ccgcggtatc taaacgcgcg
     3961 ggcaggtcat cctgtaagtc tccggcaaaa agaatacggc tttgttcgaa atcatcactg
     4021 tgacgcagca agacttcact tgccggggta aatgcagaca tggaatgctc ctcaattaat
     4081 actggcggcg attatagcca tatgttggcg cggtatcgac gaatttgcta tatttgcgcc
     4141 cctgacaaca ggagcgattc gctatgacat cccgacgaga ctggcagtta cagcaactgg
     4201 gcattaccca gtggtcgctg cgtcgccctg gcgcgttgca gggggagatt gccattgcga
     4261 tcccggcaca cgtccgtctg gtgatggtgg caaacgatct tcccgccctg accgatccta
     4321 tagtgagcga tgttctgcgc gcgttaaccg tcagccccga tcaagtgctg caactgacgc
     4381 cagaaaaaat cgcgatgctg ccgcaaggca gtcgctgcaa cagctggcgg ttgggtactg
     4441 acgaaccgct atcactggaa ggcgctcagg tggcatcacc ggcgctcacc gaattacggg
     4501 caaacccaac ggcacgcgcc gcgttatggc aacaaatttg cacatatgaa cacgatttct
     4561 tccctcgaaa cgactgattt accagcggct taccacattg aacaacgcgc ccacgccttt
     4621 ccgtggagtg aaaaaacctt tgccagcaac cagggcgagc gttatctcaa ctttcagtta
     4681 acgcaaaacg gcaaaatggc ggcgtttgcg attacgcaag tggtgctgga tgaagctaca
     4741 ttgttcaata ttgcggtcga tcctgactat cagcgtcagg gattgggaag ggcgctgctg
     4801 gaacatctga tcgacgaact ggaaaaacgc ggcgtggcga cgctatggct ggaagtccgt
     4861 gcttcaaacg ctgccgccat tgccctgtac gaaagtttag gctttaacga ggcgacaatt
     4921 cgccgcaatt actaccccac cacggacggt cgcgaagacg ccatcatcat ggcgttgcca
     4981 atcagtatgt aagacaaggt ggaataatga agtgggactg gattttcttt gatgccgatg
     5041 aaacgctgtt tacctttgac tcattcaccg gcttgcagcg gatgtttctt gattacagcg
     5101 tcacctttac cgctgaagat tttcaggact atcaggccgt taacaagcca ctgtgggtgg
     5161 attatcaaaa cggcgcgatc acttcattac agcttcagca cgggcggttt gagagctggg
     5221 ccgaacggct gaacgtcgag ccgggtaaac tcaacgaagc ctttattaat gcgatggcgg
     5281 aaatctgtac gccactgccg ggcgcggttt ctctgcttaa cgccattcgt ggcaacgcta
     5341 aaattggcat catcaccaac ggctttagcg ccttgcagca agtgcgtctg gaacgcacgg
     5401 gcctgcgtga ttatttcgat ttgctggtga tttccgaaga agttggcgtt gccaaaccga
     5461 ataagaaaat tttcgattat gcgctggaac aggcaggcaa tcctgaccgt tcgcgtgtgc
     5521 tgatggttgg cgacacagct gagtccgata ttctcggtgg catcaacgcc gggcttgcga
     5581 cctgctggct gaatgcgcac aatcgcgagc aaccagaagg catcgcgccc acctggaccg
     5641 tttcttcgtt gcacgaactg gagcagctcc tgtgtaaaca ctgattgcct cccccccgtt
     5701 gatgggtaaa atagccgcaa tttttcgttt tcaacaagcg cggcgcgatg ccgcttactc
     5761 aagaagaaag aattatgacg ttgtctcctt atttgcaaga ggtggcgaag cgccgcactt
     5821 ttgccattat ttctcacccg gacgccggta agactaccat caccgagaag gtgctgctgt
     5881 tcggacaggc cattcagacc gccggtacag taaaaggccg tggttccaac cagcacgcta
     5941 agtcggactg gatggagatg gaaaagcagc gtgggatctc cattactacg tctgtgatgc
     6001 agtttccgta tcacgattgc ctggttaacc tgctcgacac cccggggcac gaagacttct
     6061 cggaagatac ctatcgtacc ctgacggcgg tggactgctg cctgatggtt atcgacgccg
     6121 caaaaggtgt tgaagatcgt acccgtaagc tgatggaagt tacccgtctg cgcgacacgc
     6181 cgatcctcac ctttatgaac aaacttgacc gtgatatccg cgacccgatg gagctgctcg
     6241 atgaagttga gaacgagctg aaaatcggct gcgcaccgat cacctggccg attggctgcg
     6301 gcaagctgtt taaaggcgtt taccaccttt ataaagatga aacctatctc tatcagagcg
     6361 gtaaaggcca cacgattcag gaagtccgca ttgttaaagg gctgaataac ccggatctcg
     6421 acgctgcggt tggtgaagat ctagcacagc agctacgtga cgaactggaa ctggtgaaag
     6481 gcgcgtctaa cgagttcgac aaagagctgt tccttgcggg cgaaatcact ccggtattct
     6541 tcggtactgc gctgggtaac ttcggcgttg atcatatgct ggatggcctg gtggagtggg
     6601 ctcctgcgcc gatgccgcgt cagactgata cccgtaccgt agaggcgagc gaagacaaat
     6661 ttaccggctt cgtatttaaa attcaggcca acatggaccc gaaacaccgc gaccgcgtgg
     6721 cgtttatgcg tgtggtgtcc ggtaaatatg aaaaaggcat gaagctgcgc caggtgcgta
     6781 ctgcgaaaga tgtggtgatc tccgacgcgc tgacctttat ggcgggtgac cgttcgcacg
     6841 ttgaagaagc gtatcctggc gatatccttg gtctgcacaa ccacggcacc attcagatcg
     6901 gcgacacctt tacccagggt gagatgatga agttcaccgg tattccgaac ttcgcgccag
     6961 aactgttccg tcgtatccgc ctgaaagatc cgctgaagca aaaacagctg cttaaagggc
     7021 tggtacagct ttccgaagag ggcgcggtgc aggtgttccg tccgatctcc aacaacgatc
     7081 tgatcgttgg tgcggttggt gtgctgcagt ttgatgtggt ggtagcgcgt ctgaagagcg
     7141 aatacaacgt tgaagcagtg tatgagtcag tcaacgttgc cactgcccgc tgggtagaat
     7201 gtgcggacgc gaagaaattc gaagagttca agcgtaagaa cgaaagccaa ctggcgcttg
     7261 atggcggcga taacctcgct tacatcgcta ccagcatggt caacctgcgc ctggcgcagg
     7321 aacgttatcc agacgttcag ttccaccaga cccgcgagca ttaattcttg cctccagggc
     7381 gcggtagccg ctgcgccctg tcaatttccc ttccttatta gccgcttacg gaatgttctt
     7441 aaaacattca cttttgctta tgttttcgct gatatcccga gcggtttcaa aattgtgatc
     7501 tatatttaac aaagtaatga catttctgac ggcgttaaat accgttcaat gcgtagatat
     7561 cagtatctaa agccgttgat tgtcattcta ccgatattaa taactgattc agaggctgta
     7621 atggtcgtta ttcatcaccc atcgcttttg tgatggcggc cattgacttc tgtagagggt
     7681 gaagtctctc cctattcagc aatgcaacct cgtgttgcca ggctcaaatt acgagcaaac
     7741 atacaggaat aaatcgatga ctatgacaag actgaagatt tcgaaaactc tgctggctgt
     7801 aatgttgacc tctgccgtcg cgaccggctc tgcctacgcg gaaaacaacg cgcagactac
     7861 caatgaaagc acagggcaaa aagtcgatag ctctatgaat aaagtcggta atttcatgga
     7921 tgacagcgcc atcaccgcga aagtgaaggc ggccctggtg gatcatgaca acatcaagag
     7981 caccgatatc tctgtcaaaa ccgatcaaaa agtcgtgacc ctgagcggtt tcgttgaaag
     8041 ccaggctcag gccgaagagg cagtgaaagt ggcgaaaggc gttgaagggg tgacctctgt
     8101 cagcgacaaa ctgcacgttc gcgacgctaa agaaggctct gtgaagggct acgcgggtga
     8161 caccgccacc accagtgaaa tcaaagccaa actgctggcg gatgacattg tcccttcccg
     8221 tcatgtgaaa gttgaaacca ccgacggcgt ggttcagctc tccggtaccg tcgattccca
     8281 tgcacagagc gaccgtgctg aaagtatcgc caaagcggta gatggtgtga aaagcgttaa
     8341 aaatgatctg aaaactaagt aattcgtcgt aattcgacct cccgaacttt gtcgggaggc
     8401 gcgatgtgca ccacactaaa aatatcgcgt tgagtgattt tcacgctcat atcaagcggt
     8461 gacattaact atggtaaagg agacgcttat gtttcgttgg ggcatcatat ttctggttat
     8521 cgcgttaatc gccgccgcac ttggatttgg tggtctggcc ggtactgctg caggcgcagc
     8581 taaaattgtc tttgtcgtcg ggattattct gttcctggtg agtttgttca tgggccgaaa
     8641 acgaccctag atttcagacc attaagaaaa ttttatccaa agccagtcca gcggactggc
     8701 ttttgcggtt ttagcgaata ataaattgcg ttactttgtc attctttcac aacaaaaaca
     8761 ggaaggcaga ggtggggcag cgaatacctg taacgcttgg taacattgcg ccgttgtcgc
     8821 taaggccgtt ccagcctgga cgaatagctc tggtgtgcga aggcggcggg cagcgtggaa
     8881 ttttcacggc tggcgtgctg gatgagttta tgcgcgcgca gtttaatcct ttcgatcttt
     8941 atctcggcac atctgccggg gcgcagaacc tctcggcgta tatctgcaat cagcccggtt
     9001 acgcgcgcaa agtcatcatg cgctatacca caaaacgcga atttttcgat ccattgcgct
     9061 ttgtccgtgg aggaaatctt atcgatctcg actggctggt ggaggccacc gcaagccaga
     9121 tgccgttgca aatggatacc gccgcgcggt tgtttgacag cggcaaatcg ttttatatgt
     9181 gcgcctgtcg tcaggatgac tatgcgccga attacttttt accaaccaaa caaaactggc
     9241 tggatgtgat tcgcgcctcc agtgcgatac ctggctttta tcgtagcgga gtgtcgctgg
     9301 aaggcattaa ctacctggat ggtgggatca gtgatgcgat tccggttaaa gaggcggcaa
     9361 ggcagggcgc taaaacgttg gtcgtcattc gcactgtgcc gtcacaaatg tactacacgc
     9421 cgcagtggtt caaacgcatg gaacgctggc tgggtgacag tagcctgcag ccgctggtca
     9481 atctggtgca gcatcatgaa accagctatc gtgagattca gcaatttatt gagaaaccac
     9541 cgggccagct gcggatattc gaaatttatc cgccgaagcc gttgcatagt atcgcgcttg
     9601 gcagtcggat tccggcgctg cgtgaagact ataaacttgg gcgtttatgc ggtcgttatt
     9661 ttctggcaac ggtcggtaag ttattaactg aaaaagcgcc gcttacccgc catctggtgc
     9721 cagtgatgac gccggaatcg atcgttattc cgcctgcgcc agtcgccaac gatacgctgg
     9781 ttgccgaagt gagcgatgct ccgcaggcga acgacccgac atttaacaat gaggatctgg
     9841 cttgatttgc cgttttatcg acacccactg ccattttgat tttccgccgt ttagtggcga
     9901 tgaagaggcc agcctgcaac gcgcggcaca agcgggcgta ggcaagatca ttgttccggc
     9961 gacagaggcg gaaaattttg cccgcgtgca ggcgttagcg gaaaaatatc aaccgctgta
    10021 tgccgcattg ggcttgcatc ctggtatgtt gaaaaaacat agcgatgtgt ctcttgatca
    10081 gctacagcag gcgctggaaa ggcgtccggc gaaggtggtg gcggtggggg agatcggtct
    10141 ggatctcttc ggcgacgatc cgcaatttga gaggcagcag tggttactcg acgaacaact
    10201 gaaactggcg aaacgctacg atctgccggt gatcctgcat tcacggcgca cacacgacaa
    10261 actggcgatg catcttaaac gccacgattt gccacgcact ggcgtggttc acggtttttc
    10321 cggcagcctg caacaggccg aacggtttgt actgctgggc tacaaaatcg gcgtaggcgg
    10381 tactatcacc tatccacgcg ccagtaaaac ccgcgatgtc atcgcaaaat taccgctggc
    10441 atcgttgttg ctggaaaccg acgcgccgga tatgccgctc aacggttttc aggggcagcc
    10501 taatcgcccg gaacaggccg cgcgggtgtt tgccgtgctt tgcgagttac gcccggaacc
    10561 agaagatgaa attgccgaag tgttgcttaa taacacgtat acgttgttta acgtgccgta
    10621 ggccggacaa ggcgtttacg ccgcatccgg ccgttggcgc acaatgcctg atgcgacgct
    10681 gacgcgtctt atcatgccta caagtctgtg ccgaaccgta ggccggataa ggcgttcacg
    10741 ccgcatccgg cagctggcgc acaatgcctg atgcgacgct tgacgcgtct tatcatgcct
    10801 acaggtctgt gccgatccgt aggccacatc cggcatgtca caaatatagc gccggaaata
    10861 tcaaccggct caccccgcgc tcctgtaaag cgccagccag gtgctcaaca tcttccggcg
    10921 tggcgctcgc ccagctttgc gcttcgccat acacgccgtg ggcatgaaac gcgttcaggc
    10981 gtaccggaac atcgccaagt cccttgataa acgccgccag ttcttcgatg tgttgcaaat
    11041 aatccacctg gccagggatt accagcaaac gcagttctgc cagcttgccg cgctcggcaa
    11101 gcaaacagat gctgcgctta atctgctgat tatcgcgtcc ggttaattgc tgatgacatt
    11161 cgctccccca cgctttgaga tcgagcattg cgccgtcgca caccggaagt aatttttccc
    11221 agccggtttc gctcaacata ccgttactgt ccaccagaca ggtgagatgg cacagttgcg
    11281 gatcgttttt gatggcagta aacagcgcca ccacaaacgg cagctgggtc gtggcttcgc
    11341 cgccgctcac cgttatccct tcaataaaca gcactgcttt gcggatatgg ctaagcacct
    11401 catccacgct catggattgc gccattggcg tggcatgttg cgggcacatc ttcaggcagg
    11461 tatcacattg ctcgcaaacc acagcgttcc acaccacttt gccgttaaca atctgcaacg
    11521 cctgatgcgg acactgcggc acgcactctc cgcaatcatt acagcgcccc atcgtccacg
    11581 gattgtgaca gtttttgcag cgcagattgc agccctgcaa aaacagagcc agacgactgc
    11641 ctggcccgtc aacgcaggag aaggggataa tcttactgac taaagcgcat ctgctgttca
    11701 tggcttatca cgcgcggctg gcgttccaga atacgagtgt tgcgtgcggc ttcttctccc
    11761 agccaggtgg tgttggtgcg tgaaccttcg gcgcgatatt tttctaaatc cgacaaacgc
    11821 accatataac cggtaacgcg aaccagatcg ttaccgctga cattggcggt aaattcacgc
    11881 attccggctt taaaggcacc gaggcaaagc tgtaccagcg cctgcggatt acgtttgatg
    11941 gtttcgtcga gcgtcagaat gtcgctgatg ccggaataat aataagcatg atgcggggcg
    12001 acggtttgca gatgggtgat cggatctggc tcatcaccat acggcaagcg tgcacccggc
    12061 gtggtgccaa tatcggaact gatccccgac tgtgcgtgta gcatggcgcg tttttgccag
    12121 ccatatttca cgggggtatt ggcgacaaac tccgccagtt gcgcgctgat gcgataaccc
    12181 acttcatttg cagcggcttc tttaccgtaa cgtgcgacaa ttccttcttt ctcacacagt
    12241 aagttaactg cttccgccaa tccgtacatg ccaaacattg gcacaaaacg ttcagggtta
    12301 atcagccctt ctttcaccag gaagctattc tcaaagaagt gtgattgttg atagaggaat
    12361 tcacaccgcg catcgatgat ggcgatctgc tgctggcagt agtgcggtag agtgcgtgta
    12421 aagaagtcat ccagcgattc actgcgctcg gcaatggctt tcaggttaag gcgtaccagc
    12481 gtgctgccac cacccgccag cggcagtgag ttgtaacagc tcacaatccc gtagccccct
    12541 tttgtgaaaa ttttatcatg caccggaccg ttggcgatgt gcggtttgct acattcacag
    12601 atgttcttcg ccacttccag cagtaggtca tcaggggtga tttcaggatc gtagataaag
    12661 gtcaggttcg gtgaaacctg cttcaactct gcgtctgcac gtaagatcgc gcgggtaatg
    12721 ggcgaatcag acgggccgat attggcgtgc ataaaggcgt ctggcagggt tctgtcgagg
    12781 taacgccaga aacgttttat tcgaatatcg atctcgtctt gtgttagaat tctaacatac
    12841 ggttgcaaca acgcatccag ttgccccagg tagaccggca tcgatgtgac cgatggtacg
    12901 tggtggtaaa gaatggtcag cagagagagt gcatcatcaa gatctttcgc gccttccagc
    12961 tccagccatt cggaaccgtt cgccagaaaa cgggcgtaat cgggtaagac atagcgcggt
    13021 ttgtacggcg catgaccttc aaacatatcg cagattacac cctcatccag cgcgcggcgg
    13081 gcttcggcag gaagctgcgg ataaggcaga ttgttttctg cttccagagc cagaaaatgg
    13141 cgcttctgct ccgggctaag cactgggctg gtgacaattt gctggcaacg ttgttgcagt
    13201 gcattttcat gagaagtggg catcttcttt tccttttatg ccgaaggtga tgcgccattg
    13261 taagaagttt cgtgatgttc actttgatcc tgatgcgttt gccaccactg acgcattcat
    13321 ttgaaagtga attatttgaa ccagatcgca ttacagtgat gcaaacttgt aagtagattt
    13381 ccttaattgt gatgtatatc gaagtgtgtt gcggagtaga tgttagaata ctaacaaact
    13441 cgcaaggtga attttattgg cgacaagcca ggagaatgaa atgactgatc tgaaagcaag
    13501 cagcctgcgt gcactgaaat tgatggacct gaccaccctg aatgacgacg acaccgacga
    13561 gaaagtaatt gctctgtgtc atcaggccaa aactccggtc ggcaataccg ccgctatctg
    13621 tatctatcct cgctttatcc cgattgctcg caaaacgctg aaagagcagg gcaccccgga
    13681 aatccgaatc gctacggtaa ccaacttccc acacggtaac gatgacatcg aaatcgcgct
    13741 ggcagaaacc cgtgcggcaa tcgcctacgg tgctgatgaa gttgacgttg tgttcccgta
    13801 ccgcgcgctg atggcgggta acgagcaggt tggttttgac ctggtgaaag cctgtaaaga
    13861 ggcttgcgcg gcagcgaatg tactgctgaa agtgatcatc gaaaccggcg aactgaaaga
    13921 cgaagcgctg atccgtaaag cgtctgaaat ctccatcaaa gcgggtgcgg acttcatcaa
    13981 aacctctacc ggtaaagtgg ctgtgaacgc gacgccggaa agcgcgcgca tcatgatgga
    14041 agtgatccgt gatatgggcg tagaaaaaac cgttggtttc aaaccagcgg gcggcgtgcg
    14101 tactgcggaa gatgcgcaga aatatctcgc catcgcagat gaactgttcg gtgctgactg
    14161 ggcagatgcg cgtcactacc gctttggtgc ttccagcctg ctggcaagcc tgttgaaagc
    14221 gctgggccac ggtgacggta agagcgccag cagctactaa gtaagatgct ttacgcctga
    14281 tgcgctgcgc ttatcaggcc tacgagacgt atctacccgt aggccggata aggcgtagac
    14341 gcatccggca aaagccgcct catactcttt tcctcgggag gttaccttgt ttctcgcaca
    14401 agaaattatt cgtaaaaaac gtgatggtca tgcgctaagc gatgaagaaa ttcgtttctt
    14461 tatcaacggt attcgcgaca acactatctc cgaagggcag attgccgccc tcgcgatgac
    14521 cattttcttc cacgatatga caatgcctga gcgtgtctcg ctgaccatgg ctatgcgaga
    14581 ttcaggaacc gttctcgact ggaaaagcct gcatctgaat ggcccgattg ttgataaaca
    14641 ctcgaccggc ggcgtcggcg atgtcacttc gctgatgttg gggccgatgg tcgcagcctg
    14701 cggcggctat attccgatga tctccggtcg cggcctcggt catactggcg gtacgctcga
    14761 caaactggaa tccatccctg gcttcgacat tttcccggat gacaaccgtt tccgcgaaat
    14821 tattaaagac gtcggcgtgg cgattatcgg ccagaccagc tcactggctc cggcggataa
    14881 acgtttctac gcgacccgtg atattaccgc aaccgtggac tccatcccgc tgatcactgc
    14941 ctctattctg gcgaagaaac tggcggaagg tctggacgcg ctggtgatgg acgtgaaagt
    15001 aggtagcggc gcgtttatgc cgacctacga actctctgaa gcccttgccg aagcgattgt
    15061 tggcgtggct aacggcgctg gcgtgcgcac caccgcgctg ctcaccgata tgaatcaggt
    15121 actggcctcc agtgcaggta acgcggttga agttcgtgaa gcggtgcagt tcctgacggg
    15181 tgaatatcgt aacccgcgtc tgtttgatgt cacgatggca ctgtgcgtgg agatgctgat
    15241 ctccggcaaa ctggcgaaag atgacgccga agcgcgcgcg aaattgcagg cggtgctgga
    15301 caacggtaaa gcggcagaag tctttggtcg tatggtagcg gcacaaaaag gcccgaccga
    15361 cttcgttgag aactacgcga agtatctgcc gacagcgatg ctgacgaaag cagtctatgc
    15421 tgataccgaa gggtttgtca gtgaaatgga tacccgcgcg ctggggatgg cagtggttgc
    15481 aatgggcggc ggacgccgtc aggcatctga caccatcgat tacagcgtcg gctttactga
    15541 tatggcgcgt ctgggcgacc aggtagacgg tcagcgtccg ctggcagtta tccacgcgaa
    15601 agacgaaaac agctggcagg aagcggcgaa agcggtgaaa gcggcaatta aacttgccga
    15661 taaagcaccg gaaagcacac caactgtcta tcgccgtatc agcgaataac ggtatactaa
    15721 tctgatcatt taaatttgaa gcactgagta cggagaacat atgaaacgtg catttattat
    15781 ggtgctggac tcattcggca tcggcgctac agaagatgca gaacgctttg gtgacgtcgg
    15841 ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa ggcgaagctg accacggtcg
    15901 taaaggcccg ctcaatctgc caaatctgac ccgtcttggg ctggcgaaag cacacgaagg
    15961 ttctaccggt ttcattccgg cgggaatgga tggcaacgct gaagttatcg gcgcgtacgc
    16021 atgggcgcac gaaatgtcat ccggtaaaga caccccgtct ggtcactggg aaatcgccgg
    16081 tgtcccggtt ctgtttgagt ggggatattt ctccgatcac gaaaacagct tcccgcaaga
    16141 gctgctggat aaactggttg aacgcgctaa tctgccgggt tacctcggta actgccactc
    16201 ttccggtacg gtcattctgg atcaactggg cgaagagcac atgaaaaccg gcaagccgat
    16261 tttctatacc tccgctgact ccgtgttcca gattgcctgc catgaagaaa cctttggtct
    16321 ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag ctgaccaacg gcggctacaa
    16381 catcggtcgt gttatcgctc gtccgtttat cggcgacaaa gccggtaact tccagcgtac
    16441 cggtaaccgt cacgacctgg ctgttgagcc gccagcaccg accgtgctgc agaaactggt
    16501 tgatgaaaaa cacggccagg tggtttcggt cggtaaaatt gcagacatct atgccaactg
    16561 cggtatcacc aaaaaagtga aagcgaccgg cctggacgcg ctgtttgacg ccaccatcaa
    16621 agagatgaaa gaagcgggtg ataacaccat cgtcttcacc aacttcgttg acttcgactc
    16681 ttcctggggc caccgtcgcg acgtcgccgg ttatgctgcg ggtctggaac tgttcgaccg
    16741 ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac atcctgatcc tcaccgctga
    16801 ccacggctgt gatccgacct ggaccggtac tgaccacacg cgtgaacaca ttccggtact
    16861 ggtttacggc ccgaaagtaa aaccgggctc actgggtcac cgtgaaacct tcgcggatat
    16921 cggccagact ctggcaaaat attttggtac ttctgatatg gaatatggca aagccatgtt
    16981 ctgataatgg gtgcggcttg atgccctcac cccggccctc tcccacaggg agagggtgaa
    17041 aacatgttga taaggataaa acaatggcta ccccacacat taatgcagaa atgggcgatt
    17101 tcgctgacgt agttttgatg ccaggcgacc cgctgcgtgc gaagtatatt gctgaaactt
    17161 tccttgaaga tgcccgtgaa gtgaacaacg ttcgcggtat gctgggcttc accggtactt
    17221 acaaaggccg caaaatttcc gtaatgggcc acggtatggg tatcccgtcc tgctccatct
    17281 acaccaaaga actgatcacc gatttcggcg tgaagaaaat tatccgcgtt ggttcctgtg
    17341 gcgctgttct gccgcacgta aaactgcgcg acgtcgttat cggtatgggt gcctgcaccg
    17401 attccaaagt taaccgcatc cgttttaaag accatgactt tgccgctatc gctgacttcg
    17461 acatggtgcg taacgcggta gacgcggcta aagcactggg cgttgatgct cgcgttggta
    17521 acctgttctc cgctgacctg ttctactctc cggacggcga aatgttcgac gtgatggaaa
    17581 aatacggcat cctcggcgtg gaaatggaag ccgctggcat ctacggcgtc gctgcagaat
    17641 ttggcgcgaa agccctgacc atctgcaccg tgtctgacca catccgcact cacgagcaga
    17701 ccactgccgc tgagcgtcag accaccttca acgacatgat caaaatcgca ctggaatccg
    17761 ttctgttggg cgataaagag taattgtgtt ttgctgaaag gcgattgcct tgtgaagccg
    17821 gagcgggaga ctgctccggc tttttagtat ctattcatca atagcaactg atttatagat
    17881 ttactattat ttttcatatg gatagaaaat aaaatccatt caagtatcta ttcacgaatc
    17941 tattcattta tgttcagccg tcatccagga agggcatatc agcaactctg gtggacaacc
    18001 ataaattcct gccattttgc ttttgatgtt tgaactaagc cgttccgtct tttcgatctg
    18061 ttttagagaa ttaatggaga tacctaacat tttacttact tcagcaagcg ataatttatg
    18121 cctgatacgc acggcggtta gccaggatat accctggctg aacattaaat tgataatctc
    18181 ccgttcctgg ttttccagat cgatcatatt tccttccctg gatattttaa tcagcgagtt
    18241 accttaccgc ccctgccatc cacgctgaca actcccgtag ctctttttcc tgttccggga
    18301 agtcaaccag cagcgcttcg cactcctgtt gcagcatatc cgcgcggtac agacagcctt
    18361 gcagtcgccc ggcgagggct tccagcggcg cggggttgag gctgtcggta aacacctggg
    18421 cgcgggtgat atggcctttt tcaacgtcga aatgcagttc cacgccgccc caggtaaagc
    18481 gttcatccag cagatgcgag aatgccggag cctgaccgaa gttccattcc cagctactct
    18541 ggcgggcaaa ggtttcggcg aagtttggca agtctggcgt tttgtccggg gagatgattt
    18601 ccgcttccac gcgctcgcca taatgggcga aaaaggcctc ggttatggcc tcgcaaatct
    18661 gctcatgggt gatccccggc agcaattcgg tcagattggt cacacgggaa cgcactgagg
    18721 taatgccttt cgccgccagt ttctttttat ccggattgag atagtttgcc aggcggctaa
    18781 ggtcggcatt gagtagcaag gtgccgtgat ggaagccgcg atctttggtt tcgcgatagg
    18841 ctgagcctga gactttgcgg tcgccttcgg cggttttcac caccagatcg ttacgtccgg
    18901 acgcttcggc gctgacgccg agcgcgttca gagcattgag cacaatcgac gtggagatgg
    18961 ttttatcgta ctccggcttg ccagccataa aggtaaagca ggtattgccg agatcgtgga
    19021 acaccgcgcc gccaccgcta ctgcgccgcg ccaggcggac gttatcttct tccatccgcc
    19081 gggtattaca ctctttccac gggttctgcg cgcgaccaat cactaccgtg tcggcattgc
    19141 gccagagaaa cagaacgcgc tgcgtggcgg gcatctggcg aaaaatacac tcctccaccg
    19201 ccaggttaaa ccacgggtcg taagagtcag agatgagcag gcgtaatgtg gacataacga
    19261 tttcctttct tgtagtaatg ggtcactctt ttttctcgct ttcttcctct tccggcaccg
    19321 gtttactggc ggttaacagg aagggcgatt gctgccagcg ggtgcgttta ccctgtagca
    19381 gcgtgcgggt cagcactacg ccgattgcca gcgagagcag caacatcagg cgtaaaatgt
    19441 tagtggtgtt atccacctgt tgggcttcgg tggcgagcgt atgggtgtcg agcgtcaggc
    19501 gcagatagcc gagcggtccg tttttacccg caatgggttc gacaatctgc tggttaaaat
    19561 agccgcccgc ttttttgccg tcgagcgcca gccggtcgcg cacgtcgacg ctttcgccag
    19621 aacgggcgat aagatcgcct tgttcatcat atacgcccgc gtcgaggata cggctttcat
    19681 ccgttaactg atcgaggatc gcctgaatgc gtttttcatc tggtgactcg gtacgcatca
    19741 gcggtgcaac gttcagcgtc acctgacgcg ccagggtgcg ggccagttct tccagctgcg
    19801 gattacgctg tcgctggtgg ttttgactaa accatgacgc tccctgcatc agcgccacta
    19861 acaaggcaag acagaacagg acaatcactg cccgatgcag ccggaatttc agttttgtgc
    19921 gagccatctt ccaccctttg aaaatttgag acttaatgtt gccagaagca atggatacaa
    19981 ggtagcctca tgcgttattt tccctgcttc gaacgatttt acaggagcct taatgcctaa
    20041 cattacctgg tgcgacctgc ctgaagatgt ctctttatgg cccggtctgc ctctttcatt
    20101 aagtggtgat gaagtgatgc cactggatta ccacgcaggt cgtagcggct ggctgctgta
    20161 tggtcgtggg ctggataaac aacgtctgac ccaataccag agcaaactgg gcgcggcgat
    20221 ggtgattgtt gccgcctggt gcgtggaaga ttatcaggtg attcgtctgg caggttcact
    20281 caccgcgcgg gctacgcgcc tggcccacga agcgcagctg gatgtcgcgc cgctggggaa
    20341 aatcccgcac ctgcgcacgc cgggtttgct ggtgatggac atggattcca ccgccatcca
    20401 gattgaatgt attgatgaaa ttgccaaact ggccggaacg ggcgagaggg tggcggaggt
    20461 aaccgaacgg gcgatgcgcg gcgaactcga ttttaccgcc agcctgcgca gccgtgtggc
    20521 gacgctgaaa ggcgctgacg ccaatatcct gcaacaggtg cgtgaaaatc tgccgatgat
    20581 gccaggctta acgcagctgg tactcaagct ggaaacgctg ggctggaaag tggcgattgc
    20641 ctccggcggc tttactttct ttgctgaata cctgcgcgac aagctgcgcc tgacagccgt
    20701 ggtagccaat gaactggaga tcatggacgg taaatttacc ggcaatgtga tcggcgacat
    20761 cgttgacgcg cagtacaaag cgaaaactct gactcgcctc gcgcaggagt atgaaatccc
    20821 gctggcgcag accgtggcga ttggcgatgg agccaatgac ctgccgatga tcaaagcggc
    20881 agggctgggg attgcctacc atgccaagcc aaaagtgaat gaaaaaacgg aagtcaccat
    20941 ccgtcacgct gacctgatgg gtgtattctg catcctctct ggcagcctga atcagaagta
    21001 attgcctgcc cgtcatcctg cgggcggcac agcattaacg aggtacaccg tggcaaaagc
    21061 tccaaaacgc gcctttgttt gtaatgaatg cggggccgat tatccgcgct ggcaggggca
    21121 gtgcagcgcc tgtcatgcct ggaacaccat caccgaggtg cgtcttgctg cgtcgccaac
    21181 ggtggcgcgt aacgagcgtc tcagtggcta tgccggtagc gccggagtgg caaaagtcca
    21241 gaaactttcc gacatcagcc ttgaagagct gccgcgtttt tccaccggat ttaaagagtt
    21301 cgaccgcgta ctaggcggcg gcgtggtgcc gggaagtgcc attctgattg gcggtaaccc
    21361 aggtgccggg aaatccacgc tgctgttgca aacgctgtgc aaactggcgc agcagatgaa
    21421 aacgctgtat gtcactggcg aagagtcgct gcaacaggtg gcaatgcgcg ctcatcgcct
    21481 tggcctgccg actgacaatc tcaatatgtt gtcggaaacc agcatcgaac aaatctgcct
    21541 gattgccgaa gaagagcaac cgaagctgat ggtaattgac tcgatccagg tgatgcatat
    21601 ggcggatgta caatcatcgc ctggcagcgt ggcgcaggtg cgtgaaacgg cggcttatct
    21661 gacgcgcttc gccaaaacgc gcggcgtggc gattgtcatg gtggggcacg taaccaaaga
    21721 tggctcgctg gctggcccta aagtgctgga acactgtatc gactgttcag tgcttctgga
    21781 tggcgatgcc gactcccgtt ttcgcacctt acgcagccat aaaaaccgct tcggcgcggt
    21841 gaatgagctg ggcgtcttcg cgatgaccga acaggggctg cgtgaagtta gcaacccttc
    21901 cgccatcttt ttaagccgcg gtgatgaagt gacctccggt agctcagtga tggtggtatg
    21961 ggaaggaacg cgtccactgc tggtggagat tcaggcattg gtcgatcact cgatgatggc
    22021 gaacccacgc cgcgtggcag tggggctgga gcaaaaccgt ctggcaatcc tgctggcggt
    22081 gctgcaccgt cacggtgggt tgcaaatggc cgatcaggat gtatttgtta acgtggtcgg
    22141 cggcgtgaag gtgacggaaa ccagcgccga cttagcgtta ctgctggcga tggtttccag
    22201 cctgcgcgac agaccgctgc cgcaggatct ggtggtgttt ggtgaagtcg ggctggcagg
    22261 ggagatccgc ccggtgccca gcggccagga acgaatctct gaagcggcga aacacggttt
    22321 tcggcgggcg attgttccgg cggctaacgt gccgaaaaaa gcgccggaag ggatgcagat
    22381 ttttggcgtt aaaaaactct ccgacgcgct tagcgtgttc gacgacttat aatgagagat
    22441 acggagggag atatgtcgtc atttgattac ctgaaaactg ccatcaagca acagggctgc
    22501 acgctacagc aggtagctga tgccagcggt atgaccaaag ggtatttaag ccagttactg
    22561 aatgccaaaa ttaaaagccc cagcgcgcaa aagctggagg cgttgcaccg ttttttgggg
    22621 cttgaatttc cccgccaaaa gaaaaccatt ggtgtcgtat tcggtaagtt ctacccgcta
    22681 cataccggac atatctacct tatccagcgc gcctgtagcc aggttgacga gctgcatatc
    22741 attatgggct ttgacgatac ccgcgaccgc gcgttgtttg aagacagcgc catgtcgcag
    22801 cagcccaccg tgccggatcg tctgcgctgg ttattgcaaa cctttaaata tcagaaaaat
    22861 atccgtattc atgccttcaa cgaagagggc atggagccgt atccgcacgg ctgggatgtg
    22921 tggagcaacg gcatcaaaaa gtttatggct gaaaagggga tccagccgga cctgatctac
    22981 acctcggaag aagccgatgc gccacagtat atggaacatc tggggatcga aacggtgctg
    23041 gtcgatccga aacgcacctt tatgagtatc agcggtgcgc agatccgcga aaacccgttc
    23101 cgctactggg aatatatccc cactgaagtg aagccgtttt ttgtgcgtac cgtggcgatc
    23161 cttggcggcg agtcgagcgg taaatccacc ctggtaaaca aacttgccaa tatcttcaat
    23221 accaccagtg cgtgggaata tggtcgcgat tatgtctttt cacacctcgg cggtgatgag
    23281 atcgcattgc agtattccga ctacgataaa atcgcgctgg gacacgcaca atacattgat
    23341 tttgcagtga aatatgccaa taaagtggcg tttatcgaca ctgattttgt cactacccag
    23401 gcgttctgca aaaagtacga agggcgggag catccgttcg tgcaggcgct gatcgatgaa
    23461 taccgttttg atctggttat cttgctggag aacaacaccc cttgggtggc ggatggttta
    23521 cgcagcctcg gcagttcggt ggatcgcaaa gagttccaga acttgctggt ggagatgctg
    23581 gaagagaaca atatcgaatt tgtgcgggtt gaagaagacg attatgacag ccgtttcctg
    23641 cgctgcgtgg agctggtgcg ggagatgatg ggggagcaaa gataatcatc tttataagat
    23701 gttgagtggg attactgatt caaaagtgta cttagcactc cattttggtg tacaatgtac
    23761 ctgccaggga ggggtaatgg gtaaggcgat aatatttata gagacgccaa tgtttacgcg
    23821 tcagatcaag cagattgcaa ccgatgatga attaaaggaa ttgcaaaaag aactcatcgg
    23881 aactcccgat aaaggtgatc tcattcagca gactggtgga ttgcgtaaag tccgaatggt
    23941 tgcgggttca cagggaaaaa gtggcagtgt cagaataatt tactttctgg cgacggaaga
    24001 gattatttat ttgattatgg cctatccgaa aaatgccaaa gatagtctga cagatacgga
    24061 aaaagctcaa ctaaagaagt tgacgaaact gctcaaaggc gagatataaa atgagttttt
    24121 ttgatgagtt gaaaacctct ctggaagagg ctgtcgagat taaacaaggt ttaaaagaac
    24181 ctgcacgggt gacccgctac gaaattgcgg atgttaaagc tattcgtgag caactcaacg
    24241 tttcgcaaag tgagatggca aaagctctgg gaaccagtgt ggataccatt aaaagctggg
    24301 aatcgaagcg aagaaatcct acgggattgg ctgcaaaagt acttgccaca attaaagaaa
    24361 atcctgcctt tttccgggaa cttgcctccc attaatcatt taactaatac cggatgcgat
    24421 gctggcgcgc cgcatccggc attttacgca ttacttcgca atacgcttgt acttgatacg
    24481 cttcggctcc agcgcgtctg cgcccagcgt gcgtttcttg tactcttcgt actcggtaaa
    24541 gttaccttcg aagaactcaa ctttaccttc atcctggtag tccaggatgt gcgtggcgat
    24601 acggtcgagg aaccaacggt cgtgcgagat aaccatcgcg cagcccggga actccagcag
    24661 ggcgttttcc agcgcacgca gggtttcgat atccaggtcg ttggtcggtt cgtcgagcag
    24721 cagcatgttg ccgccaactt gcagcagctt cgccagatgc agacgaccgc gctcaccgcc
    24781 ggagagttca ccaacgcgtt taccctgatc aaccccttta aagttaaagc ggccaacgta
    24841 ggcacggctt ggcatctcgg tgttgccgat cttcatgata tccagcccgc cggaaacttc
    24901 ttcccaaacg gttttgctgt tatccattga gtcacggaac tgatcaaccg atgccagttt
    24961 caccgtttca cctaaagtga tggtgccgct gtccggctgt tcctgaccgg agatcatacg
    25021 gaacagggtc gatttacccg caccgttcgg accgatgatc ccgacgatcg ctcctttcgg
    25081 gatcgagaag ctcaggtcat caatcagcag acgatcgcca taggatttac gcaggttgtt
    25141 gacttccagc actttatcgc caagacgcgg tcccggtgga ataaacagtt cgttggtttc
    25201 gttacgtttc tgatattcgg tgctgttcag ctcttcaaaa cgcgccagac gtgctttacc
    25261 tttcgactga cggcctttag tcccctgacg tacccactcc agctctttct caatcgactt
    25321 acgacgcgca gcttcttgtg aagcttcctg cgccagacgt tgatctttct gctccagcca
    25381 ggaggagtag ttaccttccc acggaatacc ttcaccacgg tccagttcga ggatccagcc
    25441 cgccacgtta tcgaggaagt aacggtcgtg ggtaatcgcc acaacggtgc cttcgaagtc
    25501 gtgcaggaag cgttccagcc acgccacgga ttcggcatcc aggtggttcg tcggttcgtc
    25561 gagcagcagc atgtctggtt tttccagcag caggcggcac aacgccacgc gacgacgctc
    25621 accaccggag aggttagcga ttttcgcgtc ccagtccggc agacgcagcg catccgccgc
    25681 acgctccagc tgaacgttca ggttgtgacc gtcgtgagcc tgaatgatct cttccagacg
    25741 gccttgttca gcggccagct tgtcaaaatc ggcatccgga tcggcgtaca gcgcatacac
    25801 ttcatctagg cgtttcaggg cgttaaccac ttcagaaacc gcttcttcaa tggactcacg
    25861 cacggtgtgt tccgggttca gctgcggttc ctgcggcaga taaccaatct tgatatccgg
    25921 ttgcggacgc gcttcgcctt cgatgtcttt atcaatgccc gccataatgc gcagcagggt
    25981 ggacttaccc gcgccgttca gacccaggac accaattttt gccccaggga agaaactcag
    26041 agagatgttt ttcaaaatat gacgtttcgg cggaacaact ttgccgacac gatgcatggt
    26101 ataaacgaat tgagccacgt tggacttcgc ctctatgttt atcgtgatga tgagttttca
    26161 aaggcgaagt gtagcctgtt tccttatcta atcccagccg gtcgatcacc ctcgcagtat
    26221 actcgtcata cttcaagttg catgtgctgc gcttacgctc gctcacccca gtcacttact
    26281 tctgtaagct cctggggact cactcactta tcgccttcct gcaactcgaa ttatttagag
    26341 tataaaagta aaaaagtgtc cgtaacgtgg cgtaaacggc aatgactggt tagcataaat
    26401 ctattacgcg gcatgacgct gcattgatgt atttacactt agaggatgcg cttgtggaaa
    26461 aagccaaaca agttacctgg cggctgttgg ctgccggtgt ctgtctgctg acggtcagca
    26521 gcgtggcgcg agccgactca ctggatgagc agcgtagccg ctacgcccaa atcaagcagg
    26581 cctgggataa tcgacaaatg gatgtggtcg aacaaatgat gcctggactg aaggattatc
    26641 cgctttatcc ctacctggaa taccgccaga tcaccgatga cctgatgaat caaccggcgg
    26701 tgacggtgac taactttgtt cgcgctaacc ccacgcttcc tcccgctcgc acgctgcaat
    26761 ctcgtttcgt caatgaactg gcgcggcgtg aagactggcg tggcttgtta gcctttagtc
    26821 cggaaaagcc cggaactacc gaagcgcaat gtaattacta ctatgcgaaa tggaacaccg
    26881 ggcagagtga agaagcctgg caaggggcga aagagctgtg gctaaccggc aagagccagc
    26941 ctaacgcctg tgacaagtta tttagcgtct ggcgtgcgtc aggtaaacaa gatccgctgg
    27001 cgtatttaga gcgtatccgt ctggcgatga aagcgggtaa cactggactc gtaacagtgc
    27061 tggcagggca gatgcctgcc gattaccaga ctatcgcctc ggcaatcatt tcactggcga
    27121 acaaccctaa tacggtactg accttcgcgc gtacaaccgg agcgaccgat tttacccgtc
    27181 aaatggcggc ggtggcgttt gccagtgtgg cgcggcagga tgctgaaaat gcacggctga
    27241 tgatcccgtc gcttgcccag gcgcagcagc ttaatgaaga tcagattcag gagctgcgcg
    27301 atatcgtcgc ctggcgtttg atgggcaatg atgtcaccga tgagcaggcg aaatggcgcg
    27361 atgacgccat tatgcgttcg caatctactt cgcttattga acggcgcgtg cgaatggcgc
    27421 ttggcaccgg cgatcgtcgc ggcctgaata cctggctggc gcgtttgccg atggaggcga
    27481 aagagaaaga tgaatggcgt tactggcagg cggatttact gctggaacgc ggacgtgaag
    27541 ctgaagcaaa agagattttg catcaactca tgcaacagcg tggtttctac ccgatggttg
    27601 cagcacaacg catcggtgaa gagtatgagc tgaagattga taaagcgccg cagaatgttg
    27661 acagcgccct gactcagggg ccggagatgg cgcgcgtgcg cgagttgatg tactggaatc
    27721 tcgataatac cgcgcgtagc gagtgggcca atctggtgaa gagcaagtca aaaacagagc
    27781 aggcgcaact ggctcggtat gctttcaata accaatggtg ggatcttagc gttcaggcaa
    27841 cgatcgccgg gaagctttgg gatcatctgg aagagaggtt cccgctggcc tacaacgatc
    27901 ttttcaaacg ttacaccagt gggaaggaga tcccgcaaag ctatgcgatg gcgattgccc
    27961 gtcaggagag cgcctggaat ccgaaagtga aatcaccggt aggggccagc ggcctgatgc
    28021 agattatgcc gggcacggcg acccatacgg tgaagatgtt ctctattcct ggttatagca
    28081 gccctgggca attgctggat ccggaaacga acatcaacat tggcaccagt tacctgcaat
    28141 atgtttatca gcagtttggc aataaccgta ttttctcctc agcagcttat aacgccggac
    28201 ctggacgggt gcgaacctgg cttggcaaca gcgccgggcg tatcgacgct gtggcatttg
    28261 tcgagagtat tccgttctcc gagacgcgcg gttatgtgaa gaacgtgctg gcttatgacg
    28321 cttactaccg ctatttcatg ggggataaac cgacgttgat gagcgccacg gaatggggac
    28381 gtcgttactg atccgcacgt ttatgatatg ctatcgtact ctttagcgag tacaaccggg
    28441 ggaggcattt tgcttccccc gctaacaatg gcgacatatt atggcccaac aatcacccta
    28501 ttcagcagcg atggcagaac agcgtcacca ggagtggtta cgttttgtcg acctgcttaa
    28561 gaatgcctac caaaacgatc tccatttacc gttgttaaac ctgatgctga cgccagatga
    28621 gcgcgaagcg ttggggactc gcgtgcgtat tgtcgaagag ctgttgcgcg gcgaaatgag
    28681 ccagcgtgag ttaaaaaatg aactcggcgc gggcatcgcg acgattacgc gtggatctaa
    28741 cagcctgaaa gccgcgcccg tggagctgcg ccagtggctg gaagaggtgt tgctgaaaga
    28801 tcactgatac accgcattat gaaacggact cagcgccagg atcaccgcct ggtgatagac
    28861 gctggcgcgg gtaagtttcc cggcggtaaa cacgccgatc gccccttcct tacgaccaat
    28921 ctcatcaata ccggtataac gtgacatcac gggaccgagc gcctcgcctt cacgcacttt
    28981 ttccagaatc accgcaggta acggcaaagt tgccgaacgc gcctcgccgc gctggctggc
    29041 gttttcaatc accacccaac tgaaggtgct gtcgccatca atgcctgctt caatcgccac
    29101 ccaaaaatca gcctctggat gtaaacggcg ggcatttgct acccgatttc gtgcgccagc
    29161 gcgcgtttcc tcactgccaa agggttgttc cggtacaccg ctctcgacgg caacggatgc
    29221 aatatggcag gatccttcgc cgaagatctc gtgaaatgcc tgcagaatgg cctgaatttt
    29281 agcgggattg gtggtcgcac agacaacttg gtgcataatc agcattactc agaaaattaa
    29341 cgttacagca gtatacggaa aaaaagcatg ttacaggtat acctagtccg ccacggtgaa
    29401 acgcagtgga acgccgagcg acgtattcag ggccagtctg acagcccgct gaccgccaaa
    29461 ggtgagcaac aggcgatgca ggtggcaacc cgtgccaaag agcttggcat tacgcatatc
    29521 atcagtagcg atttaggacg gacccggcgc acggcggaaa tcatcgccca ggcctgcggc
    29581 tgtgacatca tctttgattc tcgcctgcgt gaattaaata tgggtgtact ggaaacaaga
    29641 aatatcgatt cactcaccga agaagaagag aactggcgtc ggcagctggt caatggcacc
    29701 gttgacgggc gtattcctga aggcgagtca atgcaggagc tcagcgatcg cgtcaatgcg
    29761 gcactggaat cttgccggga cttaccgcag ggaagccgac cgctgctggt gagccatggt
    29821 attgcactgg gatgtctggt gagtacgatt ctcggattac cagcatgggc agagcgccgc
    29881 ttacgtctgc gtaactgttc tatttcgcgc gtggattatc aggaaagcct gtggctggcg
    29941 tctggctggg tcgttgaaac cgcaggggac atctcgcatc tggacgcccc tgcattagat
    30001 gagctgcagc gttaacgacg gatcggaatc agtagttcac agcgcagatt aattgggcga
    30061 tctcccgctt tggcatcttc tgccgggtag tatcgctcaa tatcctgacc tttacggcgc
    30121 gtcaggttga gcattggcat gcacgtcccg tataccgtca ggataaactc ctgcacgccg
    30181 gttcccagac cttcataggt aaacatcaca tattcgccgc cctgcagcat caccggatgc
    30241 cccgtcagta catagccatc tgcctgatcc tgggctaacg cggtggtata gaatacttct
    30301 tgttcgtcgt ctttatcctg actcggacgc gtttcattca ggccgtagag caccggcgga
    30361 atggtcggcg cgttgccgag aaaatcgtgc cagaactgat aacgcatttc atggcggaaa
    30421 tcagagattt gctccagcga acaggagtag ctctgggtaa caccaatcag cggcgtatct
    30481 tccagggtga caaatttgtg ctctggcata gtgaattcac ccagacgtag cggcgggcga
    30541 ataccaaagg cgctccattc aggagaacgg cggtaaagtg caggagtctg ggcaaactgc
    30601 ttcttgaatg cgcgggtaaa tgtctgttga gagtcgaagc ggtattgcag cgcgatgtcc
    30661 agaatcggac gcgcagtcag gcgtagtgcg accgccgatt tcgacaaacg acgagcacga
    30721 atatacgcgc caatagcatg gccagtgaca tctttaaaca ttctctgtaa gtgccacttg
    30781 gaataacctg ctttcgccgc tacattgtcg agcgacaggg gctgatccag atgaccttcc
    30841 agccagatta aaaggtcgcg aataatgccg gcctgatcca taaaatatcc tcatcctttc
    30901 aacaacgagc acctgacatc aggtaattgg ataatagcat tttttgctgt tttagcattc
    30961 agtgtttttt tcttagtaga gtatgtttta ggacttgacg gaaataaaag tattgagatt
    31021 ttgttcttaa tcaatatgtt atttaccgtg acgaactaat tgctcgtgta atagataaaa
    31081 atggtaacaa tatgaaatac aagcatttga tcctgtcttt aagcctgata atgctggggc
    31141 cattggctca tgcagaagag attggttcgg tcgacaccgt atttaaaatg atcggcccgg
    31201 atcacaaaat tgttgtggaa gcctttgatg atcccgatgt gaaaaatgtc acctgttatg
    31261 tgagccgggc gaaaaccggt ggtattaaag ggggattggg tctggcggaa gatacctccg
    31321 atgcggccat ttcttgtcag caagtcgggc cgattgaact gtcggatcgt attaaaaacg
    31381 gaaaagctca gggcgaggta gtattcaaaa aacgcacgtc cctggtcttt aagtcgttac
    31441 aggtcgtgcg cttttatgac gccaaacgca acgcgctcgc ttatctggct tactccgaca
    31501 aagttgtaga aggttcgccg aaaaacgcga ttagcgcggt tcctgtcatg ccgtggcggc
    31561 aataacagag gcgatttatg caacgggaaa cggtctggtt agtggaagat gagcaaggga
    31621 tagccgacac gctggtctac atgttgcagc aggaaggttt tgccgtcgag gtctttgagc
    31681 gaggcttgcc ggtgctggat aaagctcgtc agcaggttcc cgatgtcatg attctcgacg
    31741 ttggtttgcc tgatatcagt ggctttgaat tgtgccgcca gttactggcg ctccatccgg
    31801 cgttacctgt actgttcctg acggcccgaa gtgaagaggt cgatcgcctg cttgggctgg
    31861 aaattggcgc tgacgactat gtggctaaac cgttttcacc ccgcgaagtg tgcgccaggg
    31921 tgcgcacctt actgcgtcgg gtgaagaagt tctcgacgcc gtctcccgtc atccgtattg
    31981 gacattttga attgaatgaa cccgcggcgc agatcagctg gtttgacacg ccattagcgc
    32041 tgacgcggta tgagttttta ttgttgaaga cgttactcaa gtcaccgggc cgcgtctggt
    32101 cccgccagca actgatggat agcgtatggg aagatgcgca ggacacctac gatcgcaccg
    32161 tcgataccca cattaaaacg ctgcgtgcca agctgcgcgc catcaacccc gatctttcac
    32221 cgattaatac tcatcgcggc atgggatata gcctgagggg cctgtaatgc gtatcggcat
    32281 gcggttgttg ctgggctatt ttttactggt ggcggtggca gcctggttcg tactggccat
    32341 ttttgtcaaa gaagttaaac cgggcgtgcg aagagcaacg gaggggacgt tgatcgacac
    32401 cgcaacgttg ctggcggagc tggcgcgtcc cgatttgctc tctggggacc caacgcatgg
    32461 gcaactggcg caggcgttta atcagctaca acatcgcccg tttcgcgcca atatcggtgg
    32521 cattaacaaa gtgcgcaatg aatatcatgt ctatatgacc gatgcgcagg gcaaagtatt
    32581 gttcgattcg gcaaataaag ccgttggaca ggattattcg cgctggaatg acgtctggct
    32641 aacgttgcgt ggtcagtatg gtgcgcgcag cacgttgcaa aatcctgccg atcccgaaag
    32701 ttctgtgatg tatgttgccg cgccgattat ggacggctcg cggcttattg gcgttttgag
    32761 cgtaggcaaa ccgaacgcgg cgatggctcc ggtcattaag cgcagcgagc ggcgaatttt
    32821 atgggccagc gccattttgt tggggattgc actggtgatt ggcgcaggca tggtttggtg
    32881 gatcaaccgc tccattgccc ggctcactcg ctatgctgat tccgtcactg acaataagcc
    32941 cgttcctctt cctgaactcg gcagtagcga gctgcgtaag cttgcgcagg cgctggaaag
    33001 tatgcgcgtg aagctggagg ggaaaaacta tattgagcaa tatatttatg cgttaactca
    33061 tgagctaaaa agcccactgg cggcgattcg tggcgcggcg gaaattttac gcgaaggtcc
    33121 gccgccggaa gtggtggctc gttttactga caacattctg acgcaaaatg cgcgtatgca
    33181 ggcattggta gaaacgttac tacgccaggc aagactggag aatcgtcagg aagtcgttct
    33241 gactgctgtt gatgtggcgg cattattccg ccgcgtcagc gaagcgcgca ccgtgcagtt
    33301 ggcagaaaaa aaaatcactc tgcatgttac gcccaccgag gttaacgttg ctgctgaacc
    33361 ggcgttactg gagcaggcgc tgggaaattt actggataac gccatcgatt ttactcccga
    33421 gagcggtcgc ataacgctaa gcgccgaagt ggatcaggaa tacgtcaccc ttaaggtgct
    33481 ggataccggt agtggtattc ctgactacgc gctgtcacgt atttttgaac gcttttactc
    33541 tttgccgcgt gcaaatgggc aaaaaagcag cggtctgggg ttggcgtttg tcagtgaggt
    33601 cgcccgtttg tttaacggcg aagtcacgct gcgcaacgtg caggaaggtg gcgtgctggc
    33661 ctcgcttcga cttcaccgtc acttcacata gcttcaaatt cttcccacat agtcttcgta
    33721 tcctgctgcc attgcaaagg agaagactat gttgaaatcc cccctgttct ggaaaatgac
    33781 tagcctgttt ggtgcagtat tgctgttgtt gattccgata atgctgattc ggcaggtgat
    33841 tgtcgaacgt gctgattacc gtagcgatgt ggaagatgcg attcgccaaa gtaccagcgg
    33901 gccgcaaaaa ctcgttgggc cgctcatcgc tattcctgtg accgagcttt atacggtgca
    33961 ggaagaggat aaaaccgtgg agcggaaacg aagttttatc catttttggt tacctgagtc
    34021 attgatggtt gatggcaatc agaacgtgga agaacgcaag atagggattt ataccggtca
    34081 ggtctggcac agtgatttaa cgctaaaagc cgatttcgat gtttcgcgtc ttagcgaact
    34141 caacgcgcca aatatcacct taggcaagcc atttattgtg attagcgtcg gggatgcgcg
    34201 tggtattggt gtggtgaaag cgcctgaagt taacggaacg gcgctgacca ttgaacccgg
    34261 caccgggtta gagcaaggcg ggcagggcgt gcatatccct ttacccgaag gggactggcg
    34321 gaagcagaac ctgaagctga atatggcact gaatttaagc ggtaccggcg atctttctgt
    34381 ggtgcctggc gggcgtaata gcgaaatgac cttaaccagc aactggccgc atcccagttt
    34441 tttaggtgat tttctaccag ccaaacggga agttagtgag tcaggttttc aggcgcaatg
    34501 gcaaagcagc tggtttgcta ataatctcgg tgagcgtttt gcttcaggca atgataccgg
    34561 ctgggaaaac ttcccggcgt ttagcgtcgc agtaacgacg ccagccgatc aataccaatt
    34621 aactgaccgg gcgactaagt acgccattct gctgattgca ctgactttta tggcgttctt
    34681 tgtttttgaa acgctcaccg cgcaacgttt acacccaatg caatatttgc tggtggggct
    34741 ttcattggtg atgttttatt tgctcttgct ggcgctttct gaacataccg gttttaccgt
    34801 ggcatggata atcgccagtc tgattggggc gataatgaac ggtatttatt tgcaggcggt
    34861 attgaaaggt tggtgcaaca gcatgttgtt taccctcgcg ctgttgttgc tggatggtgt
    34921 gatgtgggga ctgctcaact ctgccgatag cgcgctgttg ttgggaacca gtgtgctggt
    34981 ggtggcgctg gctggcatga tgtttgtgac ccgtaatatc gactggtatg cgttttcact
    35041 gccgaaaatg aaagccagta aagacgttac aatggacgat gaattacgta tctggaaata
    35101 aggttgaaaa ataaaaacgg cgctaaaaag cgccgttttt ttgacggtgg taaagccgat
    35161 taatcttcca gatcaccgca gaagcgataa ccttcaccgt gaatggtggc gatgatttcc
    35221 ggcgtatccg gcgtagattc gaaatgttta cgaatacggc ggatcgtcac gtctacagta
    35281 cggtcgtgcg gtttcagctc acggccggtc attttcttca gcagttcagc acgggactga
    35341 attttgcctg ggttttcaca gaagtgaagc atggcgcgga actcgctgcg cggcagcttg
    35401 tactgctcgc catcagggcc gatcaacgaa cggctgttga tgtccagttc ccaaccattg
    35461 aacttgtagc tttcaacgct acgacgttct tcgctgacag tacctagatt catggtacgg
    35521 gacagcaggt tgcgtgcacg aatcgtcagt tcacgcgggt tgaacggttt ggtgatgtag
    35581 tcatctgcac cgatttcgag gccgagaatt ttatcgactt cgttgtcacg gccagtcagg
    35641 aacatcaacg caacattcgc ctgctcgcgc agttcacgcg ctaacagaag accgttctta
    35701 cccggcagat tgatatccat gatcaccagg ttgatgtcat attcagagag gatctgatgc
    35761 atttccgcgc catctgtcgc ttcgaaaaca tcatagcctt ccgcttcgaa aatacttttc
    35821 aacgtgttgc gtgttaccaa ctcgtcttca acgataagaa tgtgcggggt ctgcatgttt
    35881 gctacctaaa ttgccaacta aatcgaaaca ggaagtacaa aagtccctga cctgcctgat
    35941 gcatgctgca aattaacatg atcggcgtaa catgactaaa gtacgtaatt gcgttcttga
    36001 tgcactttcc atcaacgtca acaacatcat tagcttggtc gtgggtactt tccctcagga
    36061 cccgacagtg tcaaaaacgg ctgtcatcct aaccatttta acagcaacat aacaggctaa
    36121 gaggggccgg acacccaata aaactacgct tcgttgacat atatcaagtt caattgtagc
    36181 acgttaacag tttgatgaaa tcatcgtatc taaatgctag ctttcgtcac attattttaa
    36241 taatccaact agttgcatca tacaactaat aaacgtggtg aatccaattg tcgagattta
    36301 ttttttataa aattatccta agtaaacaga aggatatata gcatttttta acaattcaac
    36361 cgttagtaca gtcaggaaat agtttagcct tttttaagat aagtaaaggg ctttttctgc
    36421 gacttacgtt aagagtttgt aaatttgcac cgcgtaataa gttgacagtg atcacccggt
    36481 tcgcggttat ttgatcaaga agagtggcaa tatgcgtata acgattattc tggtcgcacc
    36541 cgccagagca gaaaatattg gggcagcggc gcgggcaatg aaaacgatgg gttttagcga
    36601 actgcggatt gttgatagtc aggcacacct ggagccagcc gcccgttggg tcgcacatgg
    36661 atctggtgat attattgata atattaaagt tttcaagaca ctagctgaat cgttacacga
    36721 tgtcgatttc actgttgcca ccactgcgcg cagtcgggcg aaatatcatt actacgctac
    36781 gccagtagaa ctggtgccgc tgttagagga aaaatcttca tggatgagcc atgccgctct
    36841 ggtgtttggt cgcgaagatt ccgggctgac taacgaagag ttagcgttgg ctgacgttct
    36901 tactggtgtg ccgatggtgg cggattatcc ttcgctcaat ctggggcagg cggtgatggt
    36961 ctattgctat caattagcaa cattaataca acaaccggcg aaaagtgaca caacggcaga
    37021 ccaacatcaa ctgcaagctt tacgcgaacg agccatggta ttgctgacga ctctggcagt
    37081 ggcagatgac ataaaactgg tcgactggtt acaacaacgc ctggggcttt tagagcaacg
    37141 agacacggca atgttgcacc gtttgctgca tgatattgaa aaaaatatca ccaaataaaa
    37201 aacgccttag taagtatttt tcagcttttc attctgactg caacgggcaa tatgtctctg
    37261 tgtggattaa aaaaagagtg tctgatagca gcttctgaac tggttacctg ccgtgagtaa
    37321 attaaaattt tattgactta ggtcactaaa tactttaacc aatataggca tagcgcacag
    37381 acagataaaa attacagagt acacaacatc catgaaacgc attagcacca ccattaccac
    37441 caccatcacc attaccacag gtaacggtgc gggctgacgc gtacaggaaa cacagaaaaa
    37501 agcccgcacc tgacagtgcg ggcttttttt tcgaccaaag gtaacgaggt aacaaccatg
    37561 cgagtgttga agttcggcgg tacatcagtg gcaaatgcag aacgttttct gcgggttgcc
    37621 gatattctgg aaagcaatgc caggcagggg caggtggcca ccgtcctctc tgcccccgcc
    37681 aaaatcacca accacctggt ggcgatgatt gaaaaaacca ttagcggcca ggatgcttta
    37741 cccaatatca gcgatgccga acgtattttt gccgaacttt tgacgggact cgccgccgcc
    37801 cagccggggt tcccgctggc gcaattgaaa actttcgtcg atcaggaatt tgcccaaata
    37861 aaacatgtcc tgcatggcat tagtttgttg gggcagtgcc cggatagcat caacgctgcg
    37921 ctgatttgcc gtggcgagaa aatgtcgatc gccattatgg ccggcgtatt agaagcgcgc
    37981 ggtcacaacg ttaccgttat cgatccggtc gaaaaactgc tggcagtggg gcattacctc
    38041 gaatctaccg tcgatattgc tgagtccacc cgccgtattg cggcaagtcg tattccggct
    38101 gatcacatgg tgctgatggc aggtttcacc gccggtaatg aaaaaggcga actggtggtg
    38161 cttggacgca acggttccga ctactctgct gcggtgctgg ctgcctgttt acgcgccgat
    38221 tgttgcgaga tttggacgga cgttgacggg gtctatacct gcgacccgcg tcaggtgccc
    38281 gatgcgaggt tgttgaagtc gatgtcctac caggaagcga tggagctttc ctacttcggc
    38341 gctaaagttc ttcacccccg caccattacc cccatcgccc agttccagat cccttgcctg
    38401 attaaaaata ccggaaatcc tcaagcacca ggtacgctca ttggtgccag ccgtgatgaa
    38461 gacgaattac cggtcaaggg catttccaat ctgaataata tggcaatgtt cagtgtttcc
    38521 ggcccgggga tgaaaggaat ggtcggcatg gcggcgcgcg tctttgcagc gatgtcacgc
    38581 gcccgtattt ccgtggtgct gattacgcaa tcatcttccg aatacagcat cagtttctgc
    38641 gttccacaaa gcgactgtgt gcgagctgaa cgggcaatgc aggaagagtt ctacctggaa
    38701 ctgaaagaag gcttactgga gccgctggcg gtgacggaac ggctggccat tatctcggtg
    38761 gtaggtgatg gtatgcgcac cttgcgtggg atctcggcga aattctttgc cgcgctggcc
    38821 cgcgccaata tcaacattgt cgccattgct cagggatctt ctgaacgctc aatctctgtc
    38881 gtggtaaata acgatgatgc gaccactggc gtgcgcgtta ctcatcagat gctgttcaat
    38941 accgatcagg ttatcgaagt gtttgtgatt ggcgtcggtg gcgttggcgg tgcgctgctg
    39001 gagcaactga agcgtcagca aagctggctg aagaataaac atatcgactt acgtgtctgc
    39061 ggtgttgcca actcgaaggc tctgctcacc aatgtacatg gccttaatct ggaaaactgg
    39121 caggaagaac tggcgcaagc caaagagccg tttaatctcg ggcgcttaat tcgcctcgtg
    39181 aaagaatatc atctgctgaa cccggtcatt gttgactgca cctccagcca ggcagtggcg
    39241 gatcaatatg ccgacttcct gcgcgaaggt ttccacgttg ttacgccgaa caaaaaggcc
    39301 aacacctcgt cgatggatta ctaccatttg ttgcgtcatg cggcggaaaa atcgcggcgt
    39361 aaattcctct atgacactaa cgttggggct ggattaccgg ttattgagaa cctgcaaaat
    39421 ctgctcaatg caggtgatga attgatgaag ttctccggca ttctttcagg ttcgctttct
    39481 tatatcttcg gcaagttaga cgaaggcatg agtttctccg aggcgaccac gctggcgcgg
    39541 gaaatgggtt ataccgaacc ggacccgcga gatgatcttt ctggtatgga tgtggcgcgt
    39601 aaactattga ttctcgctcg tgaaacggga cgtgaactgg agctggcgga tattgaaatt
    39661 gaacctgtgc tgcccactga gtttaacgcc gagggtgatg ttgccgcttt tatggcgaat
    39721 ctgtcacagc tcgacgatct ctttgccgcg cgcgtggcga aggcccgtga tgaaggaaaa
    39781 gttttgcgct atgttggcaa tattgatgaa gatggcgtct gccgcgtgaa gattgccgaa
    39841 gtggatggta atgatccgct gttcaaagtg aaaaatggcg aaaacgctct ggccttctat
    39901 agccactatt atcagccgct gccgttggtg ctgcgcggat atggtgcggg caatgacgtt
    39961 acagctgctg gtgtctttgc cgatctgcta cgtaccctct catggaagtt aggagtctga
    40021 catggttaaa gtttatgccc cggcttccag tgccaatatg agcgtcgggt ttgatgtgct
    40081 cggggcggcg gtgacacccg ttgatggtgc attgctcgga gatgtagtca cggttgaggc
    40141 ggcagagaca ttcagtctca ataacctcgg acgctttgcc gataagctgc cgtcagaacc
    40201 tcgggaaaat atcgtttatc agtgctggga gcgtttttgc caggaactgg gtaagcaaat
    40261 tccagtggcg atgaccctgg aaaagaatat gccgatcggt tcgggcttag gctccagtgc
    40321 ctgttcggtg gtcgcggcgc tgatggcgat gaatgaacac tgcggcaagc cgcttaatga
    40381 cactcgtttg ctggctttga tgggcgagct ggaaggccgt atctccggca gcattcatta
    40441 cgacaacgtg gcaccgtgtt ttctcggtgg tatgcagttg atgatcgaag aaaacgacat
    40501 catcagccag caagtgccag ggtttgatga gtggctgtgg gtgctggcgt atccggggat
    40561 taaagtctcg acggcagaag cccgggctat tttaccagcg cagtatcgcc gccaggattg
    40621 cattgcgcac gggcgacatc tggcaggctt tattcacgcc tgctattccc gtcagcctga
    40681 gcttgccgcg aagctgatga aagatgttat cgctgaaccc taccgtgaac ggttactgcc
    40741 aggcttccgg caggcgcggc aggcggtcgc ggaaatcggc gcggtagcga gcggtatctc
    40801 cggctccggc ccgactttgt tcgctctgtg tgacaagccg gataccgccc agcgcgttgc
    40861 cgactggttg ggtaagaact acctgcaaaa tcaggaaggt tttgttcata tttgccggct
    40921 ggatacggcg ggcgcacgag tactggaaaa ctaaatgaaa ctctacaatc tgaaagatca
    40981 caatgagcag gtcagctttg cgcaagccgt aacccagggg gtgggcaaaa atcaggggct
    41041 gtttttcccg cacgacctgc cggaattcag cctgactgaa attgatgaga tgctgaagct
    41101 ggattttgtc acccgcagtg cgaagatcct ctcggcgttt attggtgatg aaatcccgca
    41161 ggaaatcctg gaagagcgcg tacgcgcggc gtttgccttc ccggctccgg tcgccaatgt
    41221 tgaaagcgat gtcggttgtc tggagttgtt tcacgggcca acgctggcat ttaaagattt
    41281 cggcggtcgc tttatggcac aaatgctgac ccatattgcg ggcgataagc cagtgaccat
    41341 tctgaccgcg acctccggtg ataccggagc ggcagtggct catgctttct acggtttgcc
    41401 gaatgtgaaa gtggttatcc tctatccacg aggcaaaatc agtccactgc aagaaaaact
    41461 gttctgtaca ttgggcggca atattgaaac tgttgccatc gacggcgatt tcgatgcctg
    41521 tcaggcgctg gtgaagcagg cgtttgatga tgaagaactg aaagtggcgc tggggctaaa
    41581 ctcagctaac tcgattaaca tcagccgttt gctggcgcag atttgctact actttgaagc
    41641 tgttgcgcag ctgccgcagg aagcgcgcaa ccagctggtt gtctcggtgc caagcggaaa
    41701 cttcggcgat ttgacggcgg gtctgctggc gaagtcactc ggtctgccgg tgaaacgttt
    41761 tattgctgcg accaacgtga acgataccgt gccacgtttc ctgcacgacg gtcagtggtc
    41821 acccaaagcg actcaggcga cgttatccaa tgcgatggat gttagccagc caaacaactg
    41881 gccgcgtgtg gaagagttgt tccgccgcaa aatctggcaa ctgaaagagc tggggtatgc
    41941 agccgtggat gatgaaacca cgcaacagac aatgcgtgag ttaaaagaac tgggctatac
    42001 ctcggagccg cacgctgccg tagcttatcg tgcgctgcgt gaccagttga atccaggcga
    42061 atatggcttg ttcctcggca ccgcgcatcc ggcgaaattt aaagagagcg tggaagcgat
    42121 tctcggtgaa acgttggatc tgccaaaaga gctggcagaa cgtgctgatt tacccttgct
    42181 ttcgcataac ctgcccgccg attttgctgc gttgcgtaaa ttgatgatga atcatcagta
    42241 acgtctattc attatctcaa tcaggccggg tttgctttta tgcagcccgg cttttttatg
    42301 aagaaaatat ggagaaaaac gacagggaaa aaggagaaat tctcaataaa tgcggtaact
    42361 tagagattag gattgcggag aataacaact gccgttctca tcgcgtaatc tccggatatc
    42421 gacccataac gggcaatgat aaaaggagta acctgtgaaa aagatgcaat ctatcgtact
    42481 cgcactttcc ctggttctgg tcgctcccat ggcagcgcag gctgcggaaa ttacgctagt
    42541 cccgtcagta aaattacaga taggcgatcg tgataatcgt ggctattact gggatggcgg
    42601 tcactggcgc gaccacggct ggtggaaaca acattatgaa tggcgaggca atcgctggca
    42661 cccacacgga ccgccgccac cgccgcgcca ccataagaaa gctcctcatg atcatcacgg
    42721 cggtcatggt ccaggcaaac atcaccgcta aatgacaaat gccgggtaac aatccggcat
    42781 tcagcgcctg atgcgacgct ggcgcgtctt atcaggccta cgttaattct gcaatatatt
    42841 gaatctgcat gcttttgtag gccggataag gcgttcacgc cgcatccggc attggctaca
    42901 aacttaacgc tgctcgtagc gtttaaacac cagttcgccg ttgctggagg aagcttcatc
    42961 aaagaagtaa ccttcgctat taaaaccagt cagttgctct ggtttggtca gccgattttc
    43021 aataatgaaa cgactcatca gaccgcgtgc tttcttagcg tagaagctga tgatcttaaa
    43081 tttgccgttc ttctcatcga ggaacaccgg cttgataatc tcggcattca atttcttcgg
    43141 cttcaccgat ttaaaatact catctgacgc cagattaatc accacattat cgccttgtgc
    43201 tgcgagcgcc tcgttcagct tgttggtgat gatatctccc cagaattgat acagatcttt
    43261 ccctcgggca ttctcaagac ggatccccat ttccagacga taaggctgca ttaaatcgag
    43321 cgggcgcagt acgccataca agccggaaag cattcgcaaa tgctgttggg caaaatcgaa
    43381 atcgtcttcg ctgaaggttt cggcctgcaa gccagtatag acatcacctt taaatgccag
    43441 aatcgcttgt cgggcattct ctggcgtgaa atctggctgc cagtcatgaa agcgagcggc
    43501 gttgataccc gccagtttgt cgctgatgcg catcagcgtg ctaatctgcg gaggcgtcag
    43561 tttccgcgct tcatggatca actgctggga attgtctaac agctccggca gcgtatagcg
    43621 cgtggtgggc aacgggcttt ggtaatcgag cgttttcgca ggtgaaataa gaatcagcat
    43681 atccagtcct tgcaggaaat ttatgccgac tttagcaaaa aaagagaatg agttgatcga
    43741 tagttgtgat tactcctgcg aaacatcatc ccacgcgtcc ggagaaagct ggcgactgat
    43801 atccggataa cgcaacggat caaacaccgg gcgcacgccg agtttacgct ggcgtagata
    43861 atcactggca atggtatgaa ccacaggcga gagcagtaaa atggcggtca aattggtaat
    43921 agccatgcag gccattatga tatctgccag ttgccacatc agcggaagac ttagcaaggt
    43981 gccgccgatg accgttgcga aggtgcagat tcgcagacac cagatcgctt tagggttgtt
    44041 caggcgtaag aagaagagat tgttttcggc ataaatgtag ttggcaacga tggagctgaa
    44101 ggcaaacaga ataaccacga gggtaacaaa ctccgcaccc caggagccca ttagcacccg
    44161 catcgccttc tggataagct gaataccttc cagcggcatg taggttgtgc cgttacccgc
    44221 cagtaatatc agcatggcgc ttgccgtaca gatgaccagg gtgtcgataa aaatgccaat
    44281 catctggaca atcccttgcg ctgcgggatg cggaggccag gacgcagctg ccgctgccgc
    44341 gtttggcgtc gaccccattc ccgcctcatt ggaaaacata ctgcgctgaa aaccgttagt
    44401 aatcgcctgg cttaaggtat atcccgccgc gccgcctgcc gcttcctgcc agccaaaagc
    44461 actctcaaaa atagaccaaa tgacgtgggg aagttgcccg atattcatta cgcaaattac
    44521 caggctggtc agtacccaga ttatcgccat caacgggaca aacccctgca tgagccgggc
    44581 gacgccataa agaccgcgag tgattgccag cagagcaaag acagcaagaa taatgcctgt
    44641 caccagcggg ggaaaatcaa aagaaaaact cagggctcgg gcaacggcgt tcgcctgaac
    44701 tccgctgaaa attatgccgt aggcgatgag caaaaagagg gcgaacagaa cgcccatcca
    44761 gcgcatcccc agcccgcgcg ccatatacca tgccggtccg ccacgaaact gcccattaac
    44821 gtcacgttct ttataaagtt gtgcaaggga acattcggca aacgaggtcg ccatgccgat
    44881 aaacgcggca acccacatcc agaagacggc acctggtcca ccggcggtaa tagccagcgc
    44941 aacgccagcc aggttgccgc tacccacgcg cgccgcaaga ctggtacaca atgactgaaa
    45001 tgaggttaaa ccgcctggct gtggatgaat gctattttta agacttttgc caaactggcg
    45061 gatgtagcga aactgcacaa atccggtacg aaaagtgaac caacaacctg cgccgaagag
    45121 caggtaaatc attaccgatc cccaaaggac gctgttaata aaggagaaaa aatctggcat
    45181 gcatatccct cttattgccg gtcgcgatga ctttcctgtg taaacgttac caattgttta
    45241 agaagtatat acgctacgag gtacttgata acttctgcgt agcatacatg aggttttgta
    45301 taaaaatggc gggcgatatc aacgcagtgt cagaaatccg aaacagtctc gcctggcgat
    45361 aaccgtcttg tcggcggttg cgctgacgtt gcgtcgtgat atcatcaggg cagaccggtt
    45421 acatccccct aacaagctgt ttaaagagaa atactatcat gacggacaaa ttgacctccc
    45481 ttcgtcagta caccaccgta gtggccgaca ctggggacat cgcggcaatg aagctgtatc
    45541 aaccgcagga tgccacaacc aacccttctc tcattcttaa cgcagcgcag attccggaat
    45601 accgtaagtt gattgatgat gctgtcgcct gggcgaaaca gcagagcaac gatcgcgcgc
    45661 agcagatcgt ggacgcgacc gacaaactgg cagtaaatat tggtctggaa atcctgaaac
    45721 tggttccggg ccgtatctca actgaagttg atgcgcgtct ttcctatgac accgaagcgt
    45781 caattgcgaa agcaaaacgc ctgatcaaac tctacaacga tgcagggatt agcaacgatc
    45841 gtattctgat caaactggct tctacctggc agggtatccg tgctgcagaa cagctggaaa
    45901 aagaaggcat caactgtaac ctgaccctgc tgttctcctt cgctcaggct cgtgcttgtg
    45961 cggaagcggg cgtgttcctg atctcgccgt ttgttggccg tattcttgac tggtacaaag
    46021 cgaataccga taagaaagag tacgctccgg cagaagatcc aggcgtggtt tctgtatctg
    46081 aaatctacca gtactacaaa gagcacggtt atgaaaccgt ggttatgggc gcaagcttcc
    46141 gtaacatcgg cgaaattctg gaactggcag gctgcgaccg tctgaccatc gcaccggcac
    46201 tgctgaaaga gctggctgag agcgaagggg ctatcgaacg taaactgtct tacaccggcg
    46261 aagtgaaagc gcgtccggcg cgtatcactg agtccgagtt cctgtggcag cataaccagg
    46321 atccaatggc agtagataaa ctggcggaag gtatccgtaa gtttgctgtt gaccaggaaa
    46381 aactggaaaa aatgatcggc gatctgctgt aatcattctt agcgtgaccg ggaagtcggt
    46441 cacgctacct cttctgaagc ctgtctgtca ctcccttcgc agtgtatcat tctgtttaac
    46501 gagactgttt aaacggaaaa atcttgatga atactttacg tattggctta gtttccatct
    46561 ctgatcgcgc atccagcggc gtttatcagg ataaaggcat ccctgcgctg gaagaatggc
    46621 tgacatcggc gctaaccacg ccgtttgaac tggaaacccg cttaatcccc gatgagcagg
    46681 cgatcatcga gcaaacgttg tgtgagctgg tggatgaaat gagttgccat ctggtgctca
    46741 ccacgggcgg aactggcccg gcgcgtcgtg acgtaacgcc cgatgcgacg ctggcagtag
    46801 cggaccgcga gatgcctggc tttggtgaac agatgcgcca gatcagcctg cattttgtac
    46861 caactgcgat cctttcgcgt caggtgggcg tgattcgcaa acaggcgctg atccttaact
    46921 tacccggtca gccgaagtcg attaaagaga cgctggaagg cgtgaaggac gctgcgggta
    46981 acgttgtggt gcatggtatt tttgccagcg taccgtactg cattcagttg ctggaagggc
    47041 catacattga aacggcaccg gaagtggttg cagcattcag accgaagagt gcaagacgcg
    47101 acgttagcga ataaaaaaat ccccccgagc ggggggatct caaaacaatt agtgggattc
    47161 accaatcggc agaacggtgc gaccaaactg ctcgttcagt acttcaccca tcgccagata
    47221 gattgcgctg gcaccgcaga tcagaccaat ccagccggca aagtggatga ttgcggcgtt
    47281 accggcaatg ttaccgatcg ccagcagggc aaacagcacg gtcaggctaa agaaaacgaa
    47341 ttgcagaacg cgtgcgcctt tcagcgtgcc gaagaacata aacagcgtaa atacgcccca
    47401 cagacccagg tagacaccaa ggaactgtgc atttggcgca tcggtcagac ccagtttcgg
    47461 catcagcaga atcgcaacca gcgtcagcca gaaagaaccg taagaggtga atgcggttaa
    47521 accgaaagtg ttgccttttt tgtactccag cagaccagca aaaatttgcg cgatgccgcc
    47581 gtagaaaatg cccatggcaa gaataatacc gtccagagca aaataaccca cgttgtgcag
    47641 gttaagcaga atggtggtca tgccgaagcc catcaggccc agcggtgccg gattagccaa
    47701 cttagtgttg cccataattc ctcaaaaatc atcatcgaat gaatggtgaa ataatttccc
    47761 tgaataactg tagtgttttc agggcgcggc ataataatca gccagtgggg cagtgtctac
    47821 gatcttttga ggggaaaatg aaaattttcc ccggtttccg gtatcagacc tgagtggcac
    47881 taaccatccg gcgcaggcag gcgatttgca gtacggctgg aattgtcacg cgataggcaa
    47941 tgccgctgac cgctttaacc ccatttagtg ccgcgcctac agggcctccc agacccgcgc
    48001 cgcgcagcaa accatgccca agtacgctca ttgctgcgtg ggtgcgtaaa atgcgggtca
    48061 attggctgga aagcaaatgc gacacgcctt ttgccaataa tttgtctttc atcagcagcg
    48121 gcagcagctc ttccagctca ttcaccctgg catcgaccgc gtgcagaaac tcctgcttat
    48181 gttcctcgtc cattttcttc caggtgttac gcagaaattg ttccagtaac tgttgctcaa
    48241 tctcaaacgt agacatctct ttgtcggctt tcagcttcaa tcgctttgaa acatcgagca
    48301 aaatggcccg atacaattta ccgtgtccac gcagtttgtt ggcgatacta tcgccaccaa
    48361 aatgctgtaa ttctccggca atcagctgcc agttgcggcg atgttgctcg ggatgtccct
    48421 ccatcgattt aaacagttcg ttgcgcatca gtacgctgga gaggcgagtt ttgccttttt
    48481 cattatgggt gagcaatcgg gcgaaatttg ccaattgttc ctcactacaa tgctggagaa
    48541 aatccagatc tgaatcattc aggtaattaa cattcatttt ttgtggcttc tatattctgg
    48601 cgttagtcgt cgccgataat tttcagcgtg gccatatccg atgagttcac cgtatgacca
    48661 gaaaaggtga tttttgagac gcagcgttta ttgtcgttat cgctgttaat gttgatccag
    48721 tcagtggttt gcccttcttt tatttctgaa ggaatattca gggtctgact ggcgctacgg
    48781 gcggctttga aataaaccga tgcaccgctt aactgtaaat cgccatggtc ggctgagagt
    48841 tgtatgcgtt taacaattcg gcaaacagga agtttcagcg ccagatcgtt ggtttcgtta
    48901 cgtggcattg caatgacgcc gaggatttta tggtcgtttg cctgcgccgt gcagcacagc
    48961 agcaggctaa tcgccaggct ggcggaaagc gtaaaaacgg atttcataag gattctctta
    49021 gtggggagag gtaggggatg aatacccact agtttactgc tgataaagag aagattcagg
    49081 tacgtaatat tttcttttta ttacaatttt tctatgaatg tcttgtctgc tattcattct
    49141 ctatatgaat aaaattgctg tcagtttcat gtcttgttct ggcatatcgc gaaatttctg
    49201 cgcaaaagca caaaaaattt ttgcatctcc cccttgatga cgtgggttac gaccccattt
    49261 agtagtcaac cgcagtgagt gagtctgcaa aaaaatgaaa ttgggcagtt gaaaccagac
    49321 gtttcgcccc tattacagac tcacaaccac atgatgaccg aatatatagt ggagacgttt
    49381 agatgggtaa aataattggt atcgacctgg gtactaccaa ctcttgtgta gcgattatgg
    49441 atggcaccac tcctcgtgta ctggagaacg ccgaaggcga tcgcaccacg ccttctatca
    49501 ttgcctatac ccaggatggt gaaactctgg ttggtcagcc ggctaaacgt caggcagtga
    49561 cgaacccgca aaacaccctg tttgcgatta aacgcctgat tggccgccgc ttccaggacg
    49621 aagaagtaca gcgtgatgtt tccatcatgc cgttcaaaat tattgctgct gataacggcg
    49681 acgcatgggt cgaagttaaa ggccagaaaa tggcaccgcc gcagatctct gctgaagtgc
    49741 tgaaaaaaat gaagaaaacc gctgaagatt acctgggtga accggtaact gaagctgtta
    49801 tcaccgtacc ggcatacttt aacgatgctc agcgtcaggc aaccaaagac gcaggccgta
    49861 tcgctggtct ggaagtaaaa cgtatcatca acgaaccgac cgcagctgcg ctggcttacg
    49921 gtctggacaa aggtactggc aaccgtacta tcgcggttta tgacctgggt ggtggtactt
    49981 tcgatatttc tattatcgaa atcgacgaag ttgacggcga aaaaaccttc gaagttctgg
    50041 caaccaacgg tgatacccac ctgggtggtg aagacttcga cagccgtctg atcaactacc
    50101 tggttgaaga attcaagaaa gatcagggca ttgacctgcg caacgatccg ctggcaatgc
    50161 agcgcctgaa agaagcggca gaaaaagcga aaatcgagct gtcttccgct cagcagaccg
    50221 acgttaacct gccgtacatc actgcagacg cgaccggtcc gaaacacatg aacatcaaag
    50281 tgactcgtgc gaaactggaa agcctggttg aagatctggt aaaccgttcc attgagccgc
    50341 tgaaagttgc actgcaggac gctggcctgt ccgtatctga tatcgacgac gttatcctcg
    50401 ttggtggtca gactcgtatg ccaatggttc agaagaaagt tgctgagttc tttggtaaag
    50461 agccgcgtaa agacgttaac ccggacgaag ctgtagcaat cggtgctgct gttcagggtg
    50521 gtgttctgac tggtgacgta aaagacgtac tgctgctgga cgttaccccg ctgtctctgg
    50581 gtatcgaaac catgggcggt gtgatgacga cgctgatcgc gaaaaacacc actatcccga
    50641 ccaagcacag ccaggtgttc tctaccgctg aagacaacca gtctgcggta accatccacg
    50701 tgctgcaggg tgaacgtaaa cgtgcagctg ataacaaatc tctgggtcag ttcaacctgg
    50761 atggtatcaa cccggcaccg cgcggcatgc cgcagatcga agttaccttc gatatcgatg
    50821 ctgacggtat cctgcacgtt tccgcgaaag ataaaaacag cggtaaagag cagaagatca
    50881 ccatcaaggc ttcttctggt ctgaacgaag atgaaatcca gaaaatggta cgcgacgcag
    50941 aagctaacgc cgaagctgac cgtaagtttg aagagctggt acagactcgc aaccagggcg
    51001 accatctgct gcacagcacc cgtaagcagg ttgaagaagc gggcgacaaa ctgccggctg
    51061 acgacaaaac tgctatcgag tctgcactga ctgcactgga aactgctctg aaaggtgaag
    51121 acaaagccgc tatcgaagcg aaaatgcagg aactggcaca ggtttcccag aaactgatgg
    51181 aaatcgccca gcagcaacat gcccagcagc agactgccgg tgctgatgct tctgcaaaca
    51241 acgcgaaaga tgacgatgtt gtcgacgctg aatttgaaga agtcaaagac aaaaaataat
    51301 cgccctataa acgggtaatt atactgacac gggcgaaggg gtatttcctc tccgcccgtg
    51361 cattcatcta ggggcaattt aaaaaagatg gctaagcaag attattacga gattttaggc
    51421 gtttccaaaa cagcggaaga gcgtgaaatc aaaaaggcct acaaacgcct ggccatgaaa
    51481 taccacccgg accgtaacca gggtgacaaa gaggccgagg cgaaatttaa agagatcaag
    51541 gaagcttatg aagttctgac cgactcgcaa aaacgtgcgg catacgatca gtatggtcat
    51601 gctgcgtttg agcaaggcgg catgggcggc ggcggttttg gcggcggcgc agacttcagc
    51661 gatatttttg gtgacgtttt cggcgatatt tttggcggcg gacgtggtcg tcaacgtgcg
    51721 gcgcgcggtg ctgatttacg ctataacatg gagctcaccc tcgaagaagc tgtacgtggc
    51781 gtgaccaaag agatccgcat tccgactctg gaagagtgtg acgtttgcca cggtagcggt
    51841 gcaaaaccag gtacacagcc gcagacctgt ccgacctgtc atggttctgg tcaggtgcag
    51901 atgcgccagg gtttctttgc cgtgcagcag acctgtccac actgtcaggg ccgcggtacg
    51961 ctgatcaaag atccgtgcaa caaatgtcat ggtcatggtc gtgttgagcg cagcaaaacg
    52021 ctgtccgtta aaatcccggc aggggtggac actggagacc gcatccgtct tgcgggcgaa
    52081 ggtgaagcgg gcgagcatgg cgcaccggca ggcgatctgt acgttcaggt tcaggttaaa
    52141 cagcacccga ttttcgagcg tgaaggtaac aacctgtatt gcgaagtccc gatcaacttc
    52201 gctatggcgg cgctgggtgg cgaaatcgaa gtaccgaccc ttgatggtcg cgtcaaactg
    52261 aaagtgcctg gcgaaaccca gaccggtaag ctgttccgta tgcgcggtaa aggcgtcaag
    52321 tctgtccgcg gtggcgcaca gggtgatttg ctgtgccgcg ttgtcgtcga aacaccggta
    52381 ggcctgaacg agaagcagaa acagctgctg caagagctgc aagaaagctt cggtggccca
    52441 accggcgagc acaacagccc gcgctcaaag agcttctttg atggtgtgaa gaagtttttt
    52501 gacgacctga cccgctaacc tccccaaaag cctgcccgtg ggcaggcctg ggtaaaacca
    52561 gggtgcgttg aagatatgcg agcacctgaa aagtggcggg aatcactccc cgccgttgct
    52621 cttactcgga ttcgtaagcc gtgaaaacag caacctccgt ctggccagtt cggatgtgaa
    52681 cctcacagag gtcttttctc gttaccagcg ccgctactac ggcggtgata cagatgacga
    52741 tcagggcgac aatcatcgcc ttatgctgct tcattgctct cttctccttg accttacggt
    52801 cagtaagagg cactctacat gtgttctgca tatagggggc ctcgggttga tagtaaaata
    52861 tcactcgggg cttttctcta tctgccgttc agctaatgcc tgagacagac agcctcaagc
    52921 acccgccgct attatatcgc tctctttaac ccattctgtt ttatcgattc taatcctgaa
    52981 gacgcctcgc atttttatgg cgtaattttt taatgattta attatttaac tttaatttat
    53041 ctcttcatcg caattattga cgacaaactg gattattttt gaagtattgg cctaacaaac
    53101 atcgccgact gacaacaaat taattattac ttttcccaat taatccctca gaaatcctta
    53161 ccttaagcta tgattatcag ggcttagggt cacacgtcag cgcttacagc cgtcaaaaac
    53221 tcatatcatc actgatgacg caaatccttc aatgcctcgt aaaaagcgaa ttattcctac
    53281 actataatct gattttaacg atgattcgtg cggggtaaga tagtaaaaac gatctattca
    53341 cctgaaagag aaataaaaag tgaaacatct gcatcgattc tttagcagtg atgcctcggg
    53401 aggcattatt cttatcattg ccgctatcct ggcgatgatt atggccaaca gcggcgcaac
    53461 cagtggatgg tatcacgact ttctggagac gccggttcag ctccgggttg gttcactcga
    53521 aatcaacaaa aacatgctgt tatggataaa tgacgcgctg atggcggtat ttttcctgtt
    53581 agtcggtctg gaagttaaac gtgaactgat gcaaggatcg ctagccagct tacgccaggc
    53641 tgcatttcca gttatcgccg ctattggtgg gatgattgtg ccggcattac tctatctggc
    53701 ctttaaccat gccgatccga ttacccgcga agggtgggcg atcccggcgg ctactgacat
    53761 tgcttttgca cttggtgtgc tggcgctgtt gggaagtcgt gttccgttag ccctgaagat
    53821 ctttttgatg gctctggcta ttatcgacga tcttggggcc atcattatca tcgcattgtt
    53881 ctacactaat gacttatcga tggcctctct tggcgtcgcg gctgtagcaa ttgcggtact
    53941 cgcggtattg aatctgtgtg gtgtacgccg cacgggcgtc tatattctgg ttggcgtggt
    54001 gttgtggact gcggtgttga aatcgggggt tcacgcaacc ctggcggggg taattgtcgg
    54061 cttctttatt cctttgaaag agaagcatgg gcgttctcca gcgaagcgac tggagcatgt
    54121 gttgcacccg tgggtggcgt atctgatttt accgctgttt gcatttgcta atgctggcgt
    54181 ttcactgcaa ggcgtcacgc tggatggctt gacctccatt ctgccattgg ggatcatcgc
    54241 tggcttgctg attggcaaac cgctggggat tagtctgttc tgctggttgg cgctgcgttt
    54301 gaaactggcg catctgcctg agggaacgac ttatcagcaa attatggcgg tggggatcct
    54361 gtgcggtatc ggttttacta tgtctatctt tattgccagc ctggcctttg gtagcgtaga
    54421 tccagaactg attaactggg cgaaactcgg tatcctggtc ggttctatct cttcggcggt
    54481 aattggatac agctggttac gcgttcgttt gcgtccatca gtttgacagg acggtttacc
    54541 ggggagccat aaacggctcc cttttcattg ttatcaggga gagaaatgag catgtctcat
    54601 atcaattaca accacttgta ttacttctgg catgtctata aagaaggttc cgtggttggc
    54661 gcagcggagg cgctttattt aacaccacaa accattaccg gacagattcg ggcgctggaa
    54721 gagcgcctgc aaggcaaatt atttaagcgc aagggacgtg gcctcgagcc cagcgagctg
    54781 ggagagctgg tctatcgcta tgccgataaa atgttcacct taagccagga aatgctggat
    54841 atcgtgaact atcgcaaaga atccaactta ttgtttgatg ttggtgtggc agatgcactt
    54901 tccaaacgtc tggtcagcag tgttctggat gccgcagttg tggaagacga gcagatccat
    54961 ctacgctgtt tcgaatcgac gcacgagatg cttttagagc agttgagtca gcataaactg
    55021 gatatgatca tctctgactg tccgatcgat tccactcagc aggaagggct gttttccatg
    55081 aaaattggcg aatgtggtgt cagtttctgg tgcactaacc cactaccaga aaagccgttt
    55141 cctgcctgtc ttgaagagcg tcgtttactt attccggggc gtcgctcaat gttggggcgt
    55201 aagctattaa actggtttaa ctcccagggc ttgaacgtcg aaattttggg tgagtttgat
    55261 gatgctgcgt tgatgaaagc ctttggggcg acgcataacg ctattttcgt tgcaccttcg
    55321 ctttacgcta atgatttcta taacgatgac tcggttgtgg agataggccg tgttgagaac
    55381 gtgatggaag agtaccacgc gatttttgcc gaaaggatga ttcagcaccc tgcagtacag
    55441 cgtatctgca atacagacta ttctgcgctg tttactccag cttcaaaata aacatttgta
    55501 ctttgcaggc cgaattttgg cctgcagatg gatataaaaa aacccgccag agcgggtttt
    55561 tttacaaagc tgacaacaac aggcaattaa gccagtttgt tgatctgtgc agtcaggtta
    55621 gccttatgac gtgcagcttt gtttttgtgg atcagacctt tagcagcctg acggtccacg
    55681 attggttgca tttcgttaaa tgctttctgt gcagcagctt tgtcgccagc ttcgatagct
    55741 gcgtatactt tcttgatgaa agtacgcatc atagagcgac ggcttgcgtt gtgcttacga
    55801 gccttttcag actgaatggc gcgcttctta gctgatttga tattagccaa ggtccaactc
    55861 ccaaatgtgt tctatatgga caattcaaag gccgaggaat atgccctttt agccttcttt
    55921 tgtcaatgga tttgtgcaaa taagcgccgt taatgtgccg gcactcgtta cgtagtgatg
    55981 gcgcaggatt ctaccagctt gcggggtgtg aatacagctt ttccgcgaga aaaattgcag
    56041 caggcggtca gtttcttccc atgatttgcg ccatagcaat gaaaagccac ttctttctga
    56101 tttcggcact caatcgccgg ttaaccttga ccgctgtaca aggtatactc ggacgatttt
    56161 cactgttttg agccagacat gaagctgata cgcggcatac ataatctcag ccaggccccg
    56221 caagaagggt gtgtgctgac tattggtaat ttcgacggcg tgcatcgcgg tcatcgcgcg
    56281 ctgttacagg gcttgcagga agaagggcgc aagcgcaact taccggtgat ggtgatgctt
    56341 tttgaacctc aaccactgga actgtttgct actgataaag ccccggcacg gctcacccgg
    56401 ctgcgggaaa aactgcgtta tcttgcagag tgtggtgttg attacgtgct gtgcgtgcgt
    56461 tttgacaggc gttttgcggc gttaaccgcg caaaacttca tcagtgatct tctggtgaag
    56521 cacttgcggg taaaatttct tgccgtaggt gacgatttcc gctttggcgc tggtcgtgaa
    56581 ggcgatttct tgttattaca gaaagcgggc atggaatacg gcttcgatat caccagcacg
    56641 caaacttttt gcgaaggtgg cgtgcgtatc agcagcaccg ccgtgcgtca ggcgcttgcg
    56701 gatgacaatc tggctctggc agaaagttta ctggggcacc cgtttgctat ctccgggcgt
    56761 gtagtccacg gtgatgaatt agggcgcact ataggtttcc cgacggcgaa tgtaccgcta
    56821 cgccgtcagg tttccccggt gaaaggggtt tatgcggtag aagtgttggg ccttggcgaa
    56881 aagccgttac ccggcgttgc aaacatcgga acacgcccaa cggttgccgg tattcgccag
    56941 caactggaag tgcatttgtt agatgttgca atggaccttt atggtcgcca tatacaagta
    57001 gtgctgcgta aaaaaatacg caatgagcag cgatttgcat cgctggacga actgaaagcg
    57061 cagattgcgc gtgatgaatt aaccgcccgc gaattttttg ggctaacaaa accggcttaa
    57121 gcctgttatg taatcaaacc gaaatacgga accgagaatc tgatgagtga ctataaatca
    57181 accctgaatt tgccggaaac agggttcccg atgcgtggcg atctcgccaa gcgcgaaccg
    57241 ggaatgctgg cgcgttggac tgatgatgat ctgtacggca tcattcgtgc agctaaaaaa
    57301 ggcaaaaaaa ccttcattct gcatgatggc cctccttatg cgaatggcag cattcatatt
    57361 ggtcactcgg ttaacaagat tctgaaagac attatcatta agtccaaagg gctttctgga
    57421 tatgactcgc cgtatgtgcc tggctgggac tgtcatggtc tgccaatcga actgaaagta
    57481 gagcaagaat acggtaagcc gggggagaaa ttcaccgccg ctgagttccg cgccaagtgc
    57541 cgcgaatacg ctgcgaccca ggttgacggt cagcgcaaag actttatccg tctgggcgtg
    57601 ctgggcgact ggtcgcaccc gtacctgacc atggacttca aaactgaagc caacatcatc
    57661 cgcgcgctgg gcaaaatcat cggcaacggt cacctgcaca aaggcgcgaa gccggtgcac
    57721 tggtgcgttg actgccgttc tgcactggca gaagcggaag ttgagtatta cgacaaaact
    57781 tctccgtcca tcgacgtcgc tttccaggcg gtcgatcagg atgcgctgaa aacgaaattt
    57841 ggcgtaagca atgttaacgg cccaatttcg ctggttatct ggaccaccac gccgtggacg
    57901 ctgcctgcta accgcgcaat ctccattgca cctgattttg attatgcgct ggtgcaaatc
    57961 gacggtcagg ccgtgatcct cgcgaaagat ctggttgaaa gcgtaatgca gcgtatcggc
    58021 gttagcgatt acaccattct tggcacggtg aaaggtgccg agctggaact gttgcgcttt
    58081 acccatccgt ttatggactt cgatgttccg gcaattctcg gcgaccacgt tacgctggat
    58141 gccggtaccg gtgccgttca taccgcgcca ggccacggtc cggacgacta tgtgatcggt
    58201 caaaaatatg gtctggaaac cgctaacccg gttggcccgg acggcactta tctgccgggc
    58261 acttacccga cgctggatgg cgtgaacgtc ttcaaagcga acgacatcgt cgttgcgctg
    58321 ctgcaggaaa aaggcgcgct gctgcacgtt gagaaaatgc agcacagcta tccgtgctgc
    58381 tggcgtcaca aaacgccgat catcttccgc gcgacgccgc agtggttcgt cagcatggat
    58441 cagaaaggtc tgcgtgcgca gtcactgaaa gagatcaaag gtgtgcagtg gatccctgac
    58501 tggggccagg cgcgtatcga gtcgatggtt gctaaccgtc ctgactggtg tatctctcgt
    58561 cagcgtacct ggggcgtgcc gatgtcactg ttcgtgcaca aagacacaga agaactgcat
    58621 ccgcgtactc tcgaactgat ggaagaagtg gcaaaacgcg ttgaagttga cggcattcag
    58681 gcgtggtggg atctcgatgc gaaagagatc ctcggcgacg aagctgacca gtatgtgaaa
    58741 gtaccggata cgctggatgt atggtttgac tccggatcta cccactcttc cgttgttgat
    58801 gtgcgtccgg aatttgccgg tcacgcagcg gacatgtatc tggaaggttc tgaccaacac
    58861 cgtggctggt tcatgtcttc cctgatgatc tctaccgcga tgaagggcaa agcgccatat
    58921 cgtcaggtac tgactcacgg ctttaccgtg gatggtcagg gtcgcaagat gtctaaatcc
    58981 atcggtaaca ccgtttcgcc gcaggatgtg atgaacaaac tgggtgcgga tattctgcgt
    59041 ctgtgggtgg catcaaccga ctacactggc gaaatggccg tttctgacga gatcctgaaa
    59101 cgtgctgccg acagctatcg tcgtatccgt aacaccgcgc gcttcctgct ggcaaacctg
    59161 aacggttttg atccggcaaa agatatggtg aaaccggaag agatggtggt actggatcgc
    59221 tgggccgtag gttgtgcgaa agcggcacag gaagacatcc tcaaggcgta cgaagcatac
    59281 gatttccacg aagtggtaca gcgtctgatg cgcttctgct ccgttgagat gggttccttc
    59341 tacctcgaca tcatcaaaga ccgtcagtat accgccaaag cggacagcgt ggcgcgtcgt
    59401 agctgccaga ctgcgctgta tcacatcgct gaagcgctgg tgcgctggat ggcaccaatc
    59461 ctctccttca ccgctgatga agtgtggggc tatctgccgg gcgaacgtga aaaatacgtc
    59521 tttaccggtg agtggtacga aggcctgttt ggtctggcag acagtgaagc gatgaacgat
    59581 gcgttctggg acgagctgtt gaaagtgcgt ggcgaagtga acaaagtcat tgagcaagcg
    59641 cgtgccgata agaaagtggg tggctcgctg gaagcggcag taaccttgta tgcagaaccg
    59701 gaactggcgg cgaaactgac cgcgctgggc gatgaattac gatttgtcct gttgacctcc
    59761 ggcgctaccg ttgcagacta taacgatgca cctgctgatg ctcagcagag cgaagtactc
    59821 aaagggctga aagtcgcgtt gagtaaagcc gaaggtgaga agtgcccacg ctgctggcac
    59881 tacacccagg atgtcggcaa ggtggcggaa cacgcagaaa tctgcggccg ctgtgtcagc
    59941 aacgtcgccg gtgacggtga aaaacgtaag tttgcctgat gagtcaatcg atctgttcaa
    60001 cagggctacg ctggctgtgg ctggtggtag tcgtgctgat tatcgatctg ggcagcaaat
    60061 acctgatcct ccagaacttt gctctggggg atacggtccc gctgttcccg tcgcttaatc
    60121 tgcattatgc gcgtaactat ggcgcggcgt ttagtttcct tgccgatagc ggcggctggc
    60181 agcgttggtt ctttgccggt attgcgattg gtattagcgt gatcctggca gtgatgatgt
    60241 atcgctcgaa ggccacgcag aagctaaaca atatcgctta cgcgctgatt attggcggcg
    60301 cactgggcaa cctgttcgac cgcctgtggc acggcttcgt tgtcgatatg atcgacttct
    60361 acgtcggcga ctggcacttc gccaccttca accttgccga tactgccatt tgtgtcggtg
    60421 cggcactgat tgtgctggaa ggttttttac cttctaaagc gaaaaaacaa taataaaccc
    60481 tgccggatgc agcctactga ttgctgcgaa atcgtaggcc gcatccggca aaaatcctca
    60541 aaatataaga gcaaacctgc atgtctgaat ctgtacagag caatagcgcc gtcctggtgc
    60601 atttcacgct aaaactcgac gatggcacca ccgccgagtc tacccgcaac aacggtaaac
    60661 cggcgctgtt ccgcctgggt gatgcttctc tttctgaagg gctggagcaa cacctgctgg
    60721 ggctgaaagt gggcgataaa accaccttct cgctggagcc agatgcggcg tttggcgtgc
    60781 cgtcaccgga cctgattcag tacttctccc gccgtgaatt tatggatgca ggcgagccag
    60841 aaattggcgc aatcatgctt tttaccgcaa tggatggcag tgagatgcct ggcgtgatcc
    60901 gcgaaattaa cggcgactct attaccgttg atttcaacca tccgctggcc gggcagaccg
    60961 ttcattttga tattgaagtg ctggaaatcg atccggcact ggaggcgtaa catgcagatc
    61021 ctgttggcca acccgcgtgg tttttgtgcc ggggtagacc gcgctatcag cattgttgaa
    61081 aacgcgctgg ccatttacgg cgcaccgata tatgtccgtc atgaagtggt gcataaccgc
    61141 tatgtggtcg atagcctgcg cgagcgtggg gctatcttta ttgagcagat tagcgaagta
    61201 ccggacggcg cgatcctgat tttctccgca cacggtgttt ctcaggcggt acgtaacgaa
    61261 gcgaaaagcc gtgatttgac ggtattcgac gccacctgtc cgctggtgac caaagtgcat
    61321 atggaagtcg cccgcgccag tcgccgtggc gaagaatcta ttctcatcgg ccacgccggt
    61381 cacccggaag tggaagggac aatgggtcag tacagcaacc cggaaggggg aatgtatctg
    61441 gtcgaatcgc cagacgatgt gtggaaactg acggtcaaaa acgaagagaa gctctccttt
    61501 atgacccaaa ccacgctgtc ggtggatgac acgtctgatg tgatcgacgc gctgcgtaaa
    61561 cgcttcccga aaattgtcgg tccgcgcaaa gatgacatct gctacgccac tactaaccgt
    61621 caggaagcgg tacgcgctct ggcagaacag gcggaagtgg tgctggtggt cggttcgaaa
    61681 aactcctcca actccaaccg tctggcggaa ctggcccagc gcatgggcaa acgcgcgttt
    61741 ttgattgacg atgcgaaaga tatccaggaa gagtgggtga aagaggttaa atgcgtcggc
    61801 gtgactgcgg gcgcatcggc tccggatatt ctggtacaga acgtagtcgc acgtttacag
    61861 cagcttagcg gtggcgaagc cattccgctg gaaggtcgtg aagaaaacat tgttttcgaa
    61921 gtgccgaaag agttgcgtgt cgatattcgt gaagtcgatt aagtcattag cagcctaagt
    61981 tatgcgaaaa tgccggtctt gttaccggca ttttttatgg agaaaacatg cgtttaccta
    62041 tcttcctcga tactgacccc ggcattgacg atgccgtcgc cattgccgcc gcgatttttg
    62101 cccccgaact cgacctgcaa ctgatgacca ccgtcgcggg taatgtctcg gttgagaaaa
    62161 cgacccgcaa cgccctgcaa ctgctgcatt tctggaatgc ggagattccg cttgcccagg
    62221 gcgcagccgt accgctggta cgcgcaccgc gtgatgcggc atctgtgcac ggtgaatcgg
    62281 gaatggctgg ctacgacttt gttgagcaca accgaaagcc actcgagata ccagcgtttc
    62341 tggcgattcg tgatgccctg ctgcgtgcac cagagcctgt tacgctggta gccattggcc
    62401 cgttaaccaa tattgcgctg ttactttcac aatgcccgga atgtaaacag tatattcgtc
    62461 gtctggtgat catgggcggt tctgctggtc gcggcaactg tacaccaaat gccgagttta
    62521 atatcgccgc cgatccagaa gcggctgcct gtgtcttccg tagcggaatc gaaattgtca
    62581 tgtgcggtct ggatgtcacc aatcaggcaa tattaacccc tgactatctc gctactttgc
    62641 cggagttgaa tcgcaccgga aaaatgctcc acgccctgtt tagtcactac cgtagcggca
    62701 gtatgcaaag tggactgcga atgcacgatc tctgcgccat cgcctggctg gtgcgcccgg
    62761 acttattcac cctcaagccg tgttttgtgg cagtggaaac gcagggcgaa tttacctctg
    62821 gcacgacggt ggttgatatc gacggttgcc tgggcaaacc agccaatgta caggtggcat
    62881 tggatctgaa tgtaaaaggc ttccagcagt gggtagctga ggtgctggct ctggtgccgt
    62941 aacctgtcac atgttatcgg catgcagtca ttcatcgact catgcctttc actgatatcc
    63001 ctccctgttt atcattaatt tctaattatc agcgtttttg gctggcggcg tagcgatgcg
    63061 ctggttactc tgaaaacggt ctatgcaaat taacaaaaga gaatagctat gcatgatgca
    63121 aacatccgcg ttgccatcgc gggagccggg gggcgtatgg gccgccagtt gattcaggcg
    63181 gcgctggcat tagaaggcgt gcagctgggc gcggcgctgg agcgcgaagg atcttcttta
    63241 ctgggcagcg acgccggtga gctggcgggt gcaggaaaaa caggcgttac tgtgcaaagc
    63301 agccttgatg cgataaaaga tgattttgat gtgtttatcg attttacccg tccggaaggt
    63361 acgctgaacc atctcgcttt ttgtcgccag catggcaaag ggatggtgat cggcactacg
    63421 gggtttgacg aagccggtaa acaggcgatt cgtgacgccg ccgccgatat tgcgattgtc
    63481 tttgccgcca attttagcgt tggcgttaac gtcatgctta agctgctgga gaaagcagcc
    63541 aaagtgatgg gtgactacac cgatatcgaa attattgaag cacatcatag acataaagtt
    63601 gatgcgccgt caggcaccgc actggcaatg ggagaggcga tcgcccacgc tctggataaa
    63661 gatctgaaag attgcgcggt ctacagtcgt gaaggccata ccggtgaacg tgtgcctggc
    63721 accattggtt ttgccaccgt gcgtgcaggt gacatcgttg gtgaacatac cgcgatgttt
    63781 gccgatattg gcgagcgtct ggagatcacc cataaggcgt ccagccgcat gacatttgct
    63841 aacggtgcgg taagatcggc tttgtggttg agtggtaagg aaagtggtct ttttgatatg
    63901 cgagatgtgc ttgatctcaa taatttgtaa ccacaaaatg tttgtgatga tgcaaaaata
    63961 acacatttaa ttttttgatt ataaagggct ttaattttta gcccttttat ttttggtgtt
    64021 atgcttttaa atttaatgca agtgctgaaa attacatgtt ttgtattctg tttttgttgt
    64081 tttaatgtaa attttgacca tttggtccac ttttttctgc tcattttgat ttcatgcaat
    64141 cttcttgctg cgcaagcgtt ttccagaaca tgttatatga tctttttgcc gcttaatgcc
    64201 tgtaaaacat gcatgagcca caaaataata taaaaaatcc cgccatctaa gttgactttt
    64261 agctcccata tctccagaat gccgccgttt gccagaaatt cgtcggtaag cagatttgca
    64321 ttgatttacg tcatcattgt gaattaatat gcaaataaag tgagtgaata ttctctggag
    64381 ggtgttttga ttaagtcagc gctattggtt ctggaagacg gaacccagtt tcacggtcgg
    64441 gccatagggg caacaggttc ggcggttggg gaagtcgttt tcaatacttc aatgaccggt
    64501 tatcaagaaa tcctcactga tccttcctat tctcgtcaaa tcgttactct tacttatccc
    64561 catattggca atgtcggcac taatgacgcc gatgaagaat cttctcaggt acatgcccaa
    64621 ggtctggtga ttcgcgacct gccgctgatt gccagcaact tccgtaatac cgaagacctc
    64681 tcttcttacc tgaagcgcca taacatcgtg gcgattgccg atatcgatac ccgtaagctg
    64741 acgcgtttac tgcgcgagaa aggcgcacag aatggctgca ttattgcggg tgacaacccg
    64801 gatgcggcgc tggcgttaga aaaagcccgc gcgttccctg gcctgaatgg catggatctg
    64861 gcaaaagaag tgactaccgc agaaccctat agctggacac aagggagctg gacgctgacc
    64921 ggcggcctgc cagaagcgaa aaaagaagac gagctgccgt tccacgtcgt ggcttatgat
    64981 tttggtgcca agcgcaacat cctgcggatg ctggtggata gaggttgtcg cctgaccatc
    65041 gttccggcac aaacttctgc ggaagatgtg ctgaaaatga atccggacgg catcttcctc
    65101 tccaacggtc ctggcgaccc ggccccgtgc gattacgcca ttaccgccat ccagaaattc
    65161 ctcgaaaccg atattccggt attcggcatc tgcctcggtc atcagctgct ggcgctggcg
    65221 agcggtgcga agactgtaaa aatgaaattt ggtcaccacg gcggcaacca tccggttaaa
    65281 gatgttgaga aaaatgtggt gatgattacc gcccagaacc acggttttgc ggtggatgaa
    65341 gcaacattac ctgcaaacct gcgtgtcaca cataaatccc tgttcgacgg tacgttacag
    65401 ggcattcatc gtaccgataa accggcgttc agcttccagg gacacccgga agccagccct
    65461 ggtccacacg acgccgcgcc gttgtttgac cactttatcg agttaattga gcagtaccgt
    65521 aaaaccgcta agtaatcagg agtaaaagag ccatgccaaa acgtacagat ataaaaagta
    65581 tcctgattct gggtgcgggc ccgattgtta tcggtcaggc gtgtgagttt gactactctg
    65641 gcgcgcaagc gtgtaaagcc ctgcgtgaag agggttaccg cgtcattctg gtgaactcca
    65701 acccggcgac catcatgacc gacccggaaa tggctgatgc gacctacatc gagccgattc
    65761 actgggaagt ggtacgcaag attattgaaa aagagcgccc ggacgcggtg ctgccaacca
    65821 tgggcggtca gacggcgctg aactgcgcgc tggagctgga acgtcagggc gtgttggaag
    65881 agttcggcgt caccatgatt ggtgccactg ccgatgcgat tgataaagca gaagaccgcc
    65941 gtcgtttcga cgtagcgatg aagaaaattg gtctggaaac cgcgcgttcc ggtatcgcac
    66001 acacgatgga agaagcgctg gcggttgccg ctgacgtggg cttcccgtgc attattcgcc
    66061 catcctttac catgggcggt agcggcggcg gtatcgctta taaccgcgaa gagtttgaag
    66121 aaatttgcgc ccgcggtctg gatctctctc cgactaaaga gctgctgatt gatgagtcgc
    66181 tgatcggctg gaaagagtac gagatggaag tggtgcgtga taaaaacgac aactgcatca
    66241 tcgtctgctc tatcgaaaac tttgatgcga tgggcatcca caccggtgac tccatcactg
    66301 tcgcgccagc ccaaacgctg accgacaaag aatatcaaat catgcgtaac gcctcgatgg
    66361 cggtactgcg tgaaatcggc gttgaaaccg gtggttccaa cgtccagttt gcggtgaacc
    66421 cgaaaaacgg tcgcctgatt gttatcgaaa tgaacccacg cgtgtctcgc tcctctgcgc
    66481 tggcgtcgaa agccactggt ttcccgattg ccaaagtggc ggcgaaactg gcggtgggtt
    66541 acaccctcga cgagctgatg aacgacatca ccggtgggcg tactccggcc tccttcgagc
    66601 cgtccatcga ctatgtggtt actaaaattc ctcgcttcaa cttcgagaaa ttcgccggtg
    66661 ctaacgaccg tctgaccact cagatgaaat cggttggcga agtgatggcg attggtcgca
    66721 cgcagcagga atccctacaa aaagcgctgc gcggcctgga agtgggcgca actggcttcg
    66781 atccgaaagt gagtctcgac gacccggaag cgctgactaa aatccgccgc gaactgaaag
    66841 atgctggcgc agagcgtatc tggtatatcg ctgatgcctt ccgcgcgggc ctgtctgtag
    66901 acggtgtatt taatttgacc aacattgacc gctggttcct ggtacagatt gaagagctgg
    66961 tgcgtctgga agagaaagtc gcagaagtgg gcatcactgg cctgcacgct gaattcctgc
    67021 gccagctgaa acgcaaaggc tttgccgatg cgcgtctggc aaaactcgcg ggcgtgcgtg
    67081 aagcagaaat ccgcaagctg cgcgaccagt atgacctgca cccggtttat aagcgcgtgg
    67141 atacctgtgc ggcagagttc gccaccgaca ccgcttacat gtactccact tatgaagaag
    67201 agtgcgaagc gaatccgtct accgaccgtg aaaaaatcat ggtgctcggc ggcggcccga
    67261 accgtatcgg tcagggtatc gaattcgact actgctgtgt acacgcctcg ctggcgctgc
    67321 gcgaagatgg ttatgaaacc attatggtta actgtaaccc ggaaaccgtc tccaccgact
    67381 acgacacctc cgaccgcctc tacttcgagc cggtaactct ggaagacgtg ctggaaattg
    67441 tgcgtattga gaagccgaaa ggcgttatcg tccagtacgg cggtcagacc ccgctgaaac
    67501 tggcgcgcgc gctggaagct gctggcgtac cggttatcgg taccagcccg gatgccatcg
    67561 accgtgcgga agaccgtgaa cgcttccagc atgcggttga gcgtctgaaa ctgaaacaac
    67621 cggcgaacgc caccgttacc accatcgaaa tggcggttga gaaagcgaaa gagattggct
    67681 acccgctggt ggtgcgtccg tcttacgttc tcggcggtcg ggcgatggaa atcgtctatg
    67741 acgaagctga cctgcgtcgc tacttccaga cggcggttag cgtgtctaac gatgcgccag
    67801 tgttgctgga tcatttcctt gatgacgcag tagaagttga cgtggatgct atctgcgacg
    67861 gcgaaatggt gctgattggc ggcatcatgg aacacattga gcaggcgggc gtgcactccg
    67921 gtgactccgc atgttcgctg ccagcctaca ccttaagtca ggaaattcag gatgtgatgc
    67981 gccagcaggt gcagaaactg gccttcgaat tgcaggtgcg cggcctgatg aacgtgcagt
    68041 ttgcggtgaa aaacaacgaa gtctacctga ttgaagttaa cccgcgtgcg gcgcgtaccg
    68101 ttccgttcgt ctccaaagcc accggcgtac cgctggcaaa agtggcggcg cgtgtgatgg
    68161 ctggcaaatc gctggctgag cagggcgtaa ccaaagaagt tatcccgccg tactactcgg
    68221 tgaaagaagt ggtgctgccg ttcaataaat tcccgggcgt tgacccgctg ttagggccag
    68281 aaatgcgctc taccggggaa gtaatgggcg tgggccgcac cttcgctgaa gcgtttgcca
    68341 aagcgcagct gggcagcaac tccaccatga agaaacacgg tcgtgcgctg ctttccgtgc
    68401 gcgaaggcga taaagaacgc gtggtggacc tggcggcaaa actgctgaaa cagggcttcg
    68461 agctggatgc gactcacggc acggcgattg tgctgggcga agcgggtatc aatccgcgtc
    68521 tggtgaacaa ggtgcatgaa ggccgtccgc acattcagga ccgtattaag aatggcgaat
    68581 atacctacat catcaacacc acctcaggtc gtcgtgcgat tgaagactcc cgcgtgatcc
    68641 gtcgcagtgc gctgcaatat aaagtacatt acgacaccac cctgaacggc ggctttgcca
    68701 ccgcgatggc gctgaatgcc gatgcgaccg aaaaagtaat ttcggtgcag gaaatgcacg
    68761 cgcagatcaa ataatagcgt gtcatggcgg atatttttca tccgctaatt tgatcgaata
    68821 actaatacgg ttctctgatg aggaccgttt tttttgccca ttaagtaaat cttttgggga
    68881 atcgatattt ttgatgacat aagcaggatt tagctcacac ttatcgacgg tgaagttgca
    68941 tactatcgat atatccacaa ttttaatatg gccttgttta attgctgcaa aacgagtcat
    69001 agccagtctt ttaatttgtg aaactggagt tcgtatgtgt gaaggatgtg ttgaaaaacc
    69061 actctatttg ttaatcgccg aatggatgat ggctgaaaac cggtgggtga tagcaagaga
    69121 gatctctatt catttcgata ttgaacacag caaggcggtt aataccctga cttatattct
    69181 gtcggaagtc acagaaataa gctgcgaagt taagatgatc cctaataagc tggaagggcg
    69241 gggatgccag tgccagcgac tggtaaaagt ggttgatatc gatgagcaaa tttacgcgcg
    69301 tctgcgcaat aacagccggg ataaattagt cggcgtaaga aagacgccgc gtattcctgc
    69361 cgttccgctc acggaactta accgcgagca gaagtggcag atgatgttgt caaagagtat
    69421 gcgtcgttaa tgtcatttcg ttgataccac gcagcctggt tgccggatgc agttcatggc
    69481 gcatcttatc cggcctacgg ttgctttcca gccaggtcgg ataaggcgtt tacgccgcat
    69541 ccggaaagat caccgtttcg gcgtcacatc cgtcgtcccc tgcaaacggg ggcgattttc
    69601 ctccatttgc ctcagtggct gcgtttcatg taatgatgca tgacagcgcc cgacaagatc
    69661 ctgatactct ttggtattca accgtttcca gtgtaactcg tcgtcactca cgctgcgtac
    69721 agcgcgggct ggcgtaccca tcaacaactg gcgtttctcg ccgcgaaagc ccgctttgac
    69781 aaagctcata gcggcaacaa tgctctcttc gccaatgacc gcgccatcca taatcacgct
    69841 gttcatcccg accagtgcat cgcgaccaat cacacaacca tgcaggatcg ctccgtgccc
    69901 gatatgaccg ttttccccaa cgatggtgtc agtgtcgcag tagccatgca taatgcagcc
    69961 atcctgaata ttggctcccg cttgcacgat caaccgcccg tagtcaccac gcagtgaggc
    70021 gagtgggccg atgtagacac cggctcccac aatcacatcg ccaatcaaga ctgcgctggg
    70081 atggacaaac gccgtcgggt gaaccaccgg aattaacccc tcaaaggcgt aatagctcac
    70141 ggttgttaac gtcctttcca cactggatcg cgcttctcgg caaacgccag cggcccttca
    70201 atggcatcct ccgaatgcag aaccgatgga tagtgtttca acacgccgct gcgaatatag
    70261 cgatacgctt cttctaccgg catttcactg gtggtgcggt agatctcttt cagcgccgca
    70321 atcgccagcg gagcgctgtt aaccagctgc tgcgccagtt cgcgggcgtt atccatcagt
    70381 tccgcctggc taaccacgcg gttgactacc ccccaacgca gcgcctcttc tgcgcccatt
    70441 cgtctgccgg tcatcaccat ttcattgacg atggcaggcg gcaggatctt cggcagacgc
    70501 agcacgccgc cgctgtcagg aacgatgccc agtttggctt ccggtagggc gaagctggcg
    70561 ttatcggcac aaacaataaa atctgccgcc agcgccagtt caaagccgcc gccaaaggca
    70621 tagccgttca cagcggcgat gaccggtttg tcgagattga aaatttcggt taatcccgca
    70681 aaacctcccg ggccaaagtc agcatccggt gcttcgcctt ctgctgccgc ttttaaatcc
    70741 cagcccgcgg aaaagaattt ctctccggca ccggtaataa tggcgacacg taattgcgga
    70801 tcgtcacgga aatttagaaa tacttcgccc atttcaaagc tggtttttgc atcaatagca
    70861 ttcgcttttg gacgatcaag ggtaatttcc agaattgatc cattgcgggt cagatgtaat
    70921 gattcactca ttccttttct ccatttttgc tttttcaggg acgacaacat ccctgcaaaa
    70981 aatgcatatt gttttagagc gtgattatta gctgacaggg tagttccctg ccgtttcatt
    71041 tatttcagat tctttctaat tattttcccc gagcaattac gtggcagatc ttttctgatc
    71101 tccagataag agggcacttt aaatttcgcc atattctgtt cacagaagca gaagaattcc
    71161 tcttcgctca atgtttcacc ttcattcagt accacaaatg ctttgatggc ttcatcgcga
    71221 atcgaatctt taatacccac aaccacgata tcctgaattt ttggatgggt ggcgatgata
    71281 ttttcgagct ccacgcagga gacattctcg ccaccgcgtt tgatcatatt gcagcggcga
    71341 tcgacgaaat aaaaaaagcc ctcttcgtcg cggtatccgg tatcgccggt atgtagccag
    71401 ccatcggctt ccagcacttt cgcagtggct ttcgggttga gaaaatactc tttgaagatg
    71461 gttttccctg gtacgccttt aatacagatt tcaccgagct caccagccgg gaggggacga
    71521 ttgtgatcgt cgcggatctc cgcttcgtag caaaagcccg cccgaccaat cgacggccag
    71581 cgccgtttat cgccagggcg atcgccgata atgcccacaa tggtttccgt catcccataa
    71641 gacgtcagca agcgaacgct gaagcgttca caaaacgcgt ctttttcctg ctccgacagg
    71701 ttgagataaa acatcacttc ccgcaggcag tgttgccgat cgttcgctga aagcggctgc
    71761 accatcaacg tacgaatcat catcggaata cattcggtaa tggtggcgcg gtagttctgc
    71821 acctgtcccc agaaggcgcg ggcgctgtat ttctcgacaa gcacaaaggt ggccccggca
    71881 gaaaacgccg ccatcgccgc agtacactgg cagtcaatat gaaacgcagg cattaccgtc
    71941 aggtagacgt catcgtcacg cagtgcacat tgccaggcgg agtaatatcc agcgaagcgc
    72001 aggttgtaat gggtaatcac cacccctttc ggtcgggagg tggtgccgga ggtgaagaga
    72061 atttccgccg tatcgtcagt cgatagcggc ggtgcatagc acaaggtggc aggttgttga
    72121 tttttcagtt gagtaaacga actcacgcca tcatcagcgg gaagtgccat atctgtcagg
    72181 caaatgtgcc gcaactgaga ggcatcttcc tgctgaatct gttgatacat aggataaaat
    72241 tgcgcactgg tcaccagcag gcacgcctgg ctattttgca ggatccacgt gctttcttcg
    72301 cgcaacaggc gggcgttaat cggcaccata atcgcaccaa tttttgccaa cccgaaccag
    72361 caaaagataa attccgggca gttgtcgaga tgtagagcaa ccttgtcgcc tttgcgaatc
    72421 cccagcgtat aaaacaggtt tgccgtgcgg ttaatctcct gatttaactc aagataacta
    72481 taccggttaa cgactccgct gctggattca caaatcagcg ccgttttatg accgtaaacg
    72541 tccgccagat cgtcccacat ttgacgtaga tgttgtccgc caatgatatc cattgcacct
    72601 ctatccattt tgttcgtttg ttattgagcg ggcgctagtc aggcaagctg actgacgcca
    72661 cgcgtttagt cctcaacttt ggccagacct ttgctgacca actcctgaat gtcgttttcg
    72721 ctgtagccga tatttttcaa aatggcagcc gtgtccatgc catgtgaggg cattccgcgc
    72781 cagatttgtc cggggttatt tttgaattta ggcatcacgt tcggcccttt gcaggtgcga
    72841 ccatccatcg tttgccactg agtgattgat tcgcgagcca catactgtgg attgctttcc
    72901 agttccggta cggtcagtac tttggcacag gcgatattca gttcggcaaa gcgttcttta
    72961 acttctgcga tggtatgtgc cgccagccag gcatcgagtt tctcttctac cagtgggccg
    73021 taagggcatt cgatacggtg gataagctga gtgccttccg ggatttccgg cgtaccgagc
    73081 agatgcgcga ggccaatatc tttaaagcac tcttcaattt gagtaatgcc caccagttcc
    73141 atcacgatgt agccgtcggc gcatttgtac agaccgcaac cggcgtagta gggatcttta
    73201 cctttggtca tgcgcgggca catttctccg ccgttgaagt aatccatcat gaagtactgg
    73261 cccatacgca gcatcacttc atacatggcg atgtcgatac tttcgccttt accggtttca
    73321 cgcgctttat gcagtgctgc cagcgccgcc gtggtggcgg tcaggccaga aaagtaatcg
    73381 gcggtatacg ggaaggcagg cattggctgg tcaacatcac cgttctgaat caggtaacca
    73441 ctgaaggcct gggcgatggt gttataggcc ggaagattgg tgtactcctc ggtgccgtac
    73501 tgaccaaaac cggacaggtg agcgataacc agtttcgggt tgtgctgcca caggacttca
    73561 tcggtaatgc cacgacgggc aaaggccgga cctttactgg cttcgatgaa gatatcggtg
    73621 gtttccatta atttcagaaa cgcttcgcgg ccttcatctt tgaaaatatt taacgacagc
    73681 gcgtgcaaat tgcggcggga gagctgcggg tagttcggtt gaacgcgaat ggtgtcggcc
    73741 caggcgacgt tctcgatcca aataacttcc gcgccccatt ctgcgaacat ttgtccggca
    73801 aacggtccgg cgatttcgat accggagaag acaacgcgca atccggccag cggtccgaat
    73861 ttcggcatgg gtagatgatc cattattagc tcctgaaaaa tttatgtagc gcatgactgc
    73921 cggatgcggc gtaaacgcct tatccggccg accgtcggcc tgataagacg cgtcagcgtc
    73981 gcatcaggca gcgcacggac ttagcggtat tgcttcagca ccgcgcgacc cagcgtcagg
    74041 atctgcattt cgtcagatcc tccggagacg cggtctacac gcaggtcacg ccagaagcgg
    74101 ctgatgcggt ggttgcccgc aatcccgaca ccgcccagca cctgcattgc gctatccaca
    74161 acttcaaatg ccgcattggc gcagaagtat ttgcacatcg ctgcatcgcc agaggtgatg
    74221 gtgccgttgt ctgctttcca cgctgcttca tacagcatgt ttttcatgga gtttaatttg
    74281 atcgccatgt gggcgaattt ttcctgaatc aactggaaac gaccaatagc ctcgccaaac
    74341 tgcacgcgct ggttggcgta gcgcgccgca tcttcaaagg cgcacatcgc cgtaccgtag
    74401 ttggtcaggg ctaccaggaa acgttcatgg tcgaactctt ctttgacgcg gttaaatccg
    74461 ttaccttccc gaccgaacat gtctttctcg tccagttcca cgtcgtcaaa agtgatttca
    74521 cagcagctat ccatacgcag accgagcttc tcaagtttgg tcactttgat gcccggtttg
    74581 ctcatatcaa caaaccattc ggtgtagaca ggtttgtccg gagaagcccc gtcgcgcgcc
    74641 atcaccacga tgtacggggt gtaggcgctg ctggtaataa aacacttact accattaaga
    74701 taaatcttac catttctacg ggtataagtc gttttcaggc tacccacgtc ggagcccgcg
    74761 cccggttcgg taatcgctga gttccacatc tgcttaccgg tgccgcggaa agccataatc
    74821 ttgtcgatct gctcttgtgt gccttcgcgc aggaaggtat tgaacccgcc cggcaactgg
    74881 tacagcacat aggttggtgc ccccagacgt cccagctcca tccacacggc ggcgagagta
    74941 acaaaacccg cgtcaagacc accgtgctct tcaggaatca gcagactgtc gatacccata
    75001 tccgccagtg ctttgacaaa acgttccggg tagacgctgt cacggtcgca ctcggcaaaa
    75061 taggcctccc agttttcgct ggccatcagt tcgcggatac cggcgacaaa cagttcctgc
    75121 tcatcattta aattaaaatc catctttcaa cctcttgata ttttgggggt taattaatct
    75181 ttccagttct gtttcgcgtc tttaataaag gagagcgtca ccataatgtt gacgaagaac
    75241 agcgggcatc ctccggcgat aatggcggtt tgaatcggtt tcaggccgcc gagcgccagc
    75301 agaacaatac cgataatgcc aaccagaatt gaccagccga tacgcaccag cagcggtggt
    75361 tcttcaccat cgcgtacttc gcggcaagtg gacatcgcca gggtataaga gcaggcgtta
    75421 accagcgtaa cggtggcaat aaagcagagg atgaagaagc cccacatggt ggcggtgctg
    75481 agtggcagag cggcccaggt ttcgatgatg gcgcgcgcca caccgtactg ttcgatcaga
    75541 tttggaatgt tgatgatgtt tttatctatc aacagcagag tgttactacc gagtacagtc
    75601 cacaggatcc aggttgacgc tgtcagcccc agcaccatgc cgaagcacag ttcacgcacg
    75661 gtacgaccac gggagatgcg ggcgaggaag atgctcatct ggatagcgta aatcacccac
    75721 catgcccagt agaacacggt ccagccctgc gggaagccgc ctttagcgat gggatcggta
    75781 tagaacaaca tgcgcggcag atacatcagc aacatcccca ccgaatcggt gaagtagttc
    75841 atgatgaagc tggcaccgct gacgatgaac acccaaccca gcatcaggaa gctcaggtaa
    75901 ctacgcacgt cactggcgat acgtacccct ttttgcagac cgcaagcgac gcaaatggcg
    75961 ttgaggataa tccagcaggt aatgatgata gcgtccagtt gcagggtatg cggaatgcca
    76021 aacaaccatt gcatacactc ggtcaccagc ggcgtggcaa ggcccagact ggtacccatc
    76081 gcgaagatca aggcgacgag atagaagttg tcgacgatag tgccgaacaa ccctttggcg
    76141 tgtttttcac ctaccagcgg caccagggtg gagctggggc gaatcacttc cattttgcgg
    76201 acaaagaaga agtaagcgaa ggcgactgaa aggaagctgt aagtggccca cggcagaggt
    76261 ccccagtgga acaagctgta agccagcccc aactctttcg cgcctgttga gttcggttct
    76321 aagccaaacg gcggggtgga gatgtagtag tagatctcaa tcgatcccca gaacagtacg
    76381 gcagcagacg tacaggaggc gaacatcata aagatccaac tggcggtact aaattctggt
    76441 ggttcgttac ctaaacgctt tttggcatac gggccaaaca ccagccagaa ccagccgaaa
    76501 agcatcacca ccatatacca ttcaaatgcc catccccata cattggtgac gtaactgaat
    76561 acagcattaa taacgacatt cgccgcatcc agatctctga ctgtaagcca aaaaagtatg
    76621 ccgacgatta ttaacggcgg aaagaaaacc ttcggttcta ttcccgtttt tctcttttca
    76681 ttcttcatga gttaattcca ctgtgaaaac gaacatttat tttgcgttc
//
LOCUS       DAPHCV010000007        66457 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.007, whole genome shotgun sequence.
ACCESSION   DAPHCV010000007 DAPHCV010000000
VERSION     DAPHCV010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.007"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..461)
                     /gene="folK"
                     /locus_tag="RH033_000467"
     CDS             complement(<1..461)
                     /gene="folK"
                     /locus_tag="RH033_000467"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDV2989706.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALKTTLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELLFPDGLSLVEALQAKGF"
     gene            complement(458..1876)
                     /gene="pcnB"
                     /locus_tag="RH033_000468"
     CDS             complement(458..1876)
                     /gene="pcnB"
                     /locus_tag="RH033_000468"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HDV2989707.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            complement(1915..2811)
                     /gene="gluQRS"
                     /locus_tag="RH033_000469"
     CDS             complement(1915..2811)
                     /gene="gluQRS"
                     /locus_tag="RH033_000469"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HDV2989708.1"
                     /translation="MTDTHYIGRFAPSPSGELHFGSLIAALGSYLQARARHGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRELHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWQVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2878..3333)
                     /gene="dksA"
                     /locus_tag="RH033_000470"
     CDS             complement(2878..3333)
                     /gene="dksA"
                     /locus_tag="RH033_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HDV2989709.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3511..4215)
                     /gene="sfsA"
                     /locus_tag="RH033_000471"
     CDS             complement(3511..4215)
                     /gene="sfsA"
                     /locus_tag="RH033_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDV2989710.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAVLNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     LSAEGMALKKSLPVTL"
     gene            complement(4230..4760)
                     /gene="thpR"
                     /locus_tag="RH033_000472"
     CDS             complement(4230..4760)
                     /gene="thpR"
                     /locus_tag="RH033_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HDV2989711.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPDAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYTVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4834..7263
                     /gene="hrpB"
                     /locus_tag="RH033_000473"
     CDS             4834..7263
                     /gene="hrpB"
                     /locus_tag="RH033_000473"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HDV2989712.1"
                     /translation="MSSLPVAAVLPELLAALDCASQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDSLQQMLPEAPVIISEGRSFPVERRYLPLPTHQRFDEAVAVATAEILRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNAQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDTAQMSWLDQPPAVNLQAAKRL
                     LRMLGGLDGERLSAQGQKMAALGNDPRLAAMLVSAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7357..9891
                     /gene="mrcB"
                     /locus_tag="RH033_000474"
     CDS             7357..9891
                     /gene="mrcB"
                     /locus_tag="RH033_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HDV2989713.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYIALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10111..12300
                     /gene="fhuA"
                     /locus_tag="RH033_000475"
     CDS             10111..12300
                     /gene="fhuA"
                     /locus_tag="RH033_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HDV2989714.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEIQMHQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIASDGHTLNRGTVVDNERLQNFSVDTQLESKFATGEVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGNAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRVSYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            12351..13148
                     /gene="fhuC"
                     /locus_tag="RH033_000476"
     CDS             12351..13148
                     /gene="fhuC"
                     /locus_tag="RH033_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HDV2989715.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13148..14038
                     /gene="fhuD"
                     /locus_tag="RH033_000477"
     CDS             13148..14038
                     /gene="fhuD"
                     /locus_tag="RH033_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HDV2989716.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAIIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMIW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRILDNAIGGKA"
     gene            14035..16017
                     /gene="fhuB"
                     /locus_tag="RH033_000478"
     CDS             14035..16017
                     /gene="fhuB"
                     /locus_tag="RH033_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HDV2989717.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16052..17332)
                     /gene="hemL"
                     /locus_tag="RH033_000479"
     CDS             complement(16052..17332)
                     /gene="hemL"
                     /locus_tag="RH033_000479"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDV2989718.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LREAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17557..18978
                     /gene="clcA"
                     /locus_tag="RH033_000480"
     CDS             17557..18978
                     /gene="clcA"
                     /locus_tag="RH033_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HDV2989719.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19002..19067
                     /locus_tag="RH033_000481"
     CDS             19002..19067
                     /locus_tag="RH033_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2989720.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19060..19404
                     /gene="erpA"
                     /locus_tag="RH033_000482"
     CDS             19060..19404
                     /gene="erpA"
                     /locus_tag="RH033_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HDV2989721.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19451..20074)
                     /locus_tag="RH033_000483"
     CDS             complement(19451..20074)
                     /locus_tag="RH033_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HDV2989722.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFYVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20112..20912)
                     /gene="btuF"
                     /locus_tag="RH033_000484"
     CDS             complement(20112..20912)
                     /gene="btuF"
                     /locus_tag="RH033_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HDV2989723.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLVPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARAPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(20905..21603)
                     /gene="mtnN"
                     /locus_tag="RH033_000485"
     CDS             complement(20905..21603)
                     /gene="mtnN"
                     /locus_tag="RH033_000485"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDV2989724.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21687..23204
                     /gene="dgt"
                     /locus_tag="RH033_000486"
     CDS             21687..23204
                     /gene="dgt"
                     /locus_tag="RH033_000486"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HDV2989725.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLYSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFNKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23334..24758
                     /gene="degP"
                     /locus_tag="RH033_000487"
     CDS             23334..24758
                     /gene="degP"
                     /locus_tag="RH033_000487"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HDV2989726.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            24913..26070
                     /gene="cdaR"
                     /locus_tag="RH033_000488"
     CDS             24913..26070
                     /gene="cdaR"
                     /locus_tag="RH033_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HDV2989727.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEQR"
     gene            complement(26185..26571)
                     /locus_tag="RH033_000489"
     CDS             complement(26185..26571)
                     /locus_tag="RH033_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HDV2989728.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGIGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26729..27541)
                     /gene="yaeI"
                     /locus_tag="RH033_000490"
     CDS             complement(26729..27541)
                     /gene="yaeI"
                     /locus_tag="RH033_000490"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HDV2989729.1"
                     /translation="MITRRRFLQAAAATLAAGSSLSYMHYWEPGWLELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGVAQKPDLILLGGDYVLFDMQLNFSAFS
                     DVLSSLAECAPTFACYGNHDRPVGTEKNRLIGEALKSAGITVLFNEAMLITTQKQQFE
                     LVGTGDLWTGQCKPPPASEANLPRLVLAHNPDSKEVMRNEKWDLMLCGHTHGGQLRIP
                     LVGEPFAPIEDKRYAAGLNAFGERQIYTTRGVGSLYGLRLNCRPEVTILELV"
     gene            complement(27594..28418)
                     /gene="dapD"
                     /locus_tag="RH033_000491"
     CDS             complement(27594..28418)
                     /gene="dapD"
                     /locus_tag="RH033_000491"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HDV2989730.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28449..31121)
                     /gene="glnD"
                     /locus_tag="RH033_000492"
     CDS             complement(28449..31121)
                     /gene="glnD"
                     /locus_tag="RH033_000492"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HDV2989731.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(31183..31977)
                     /gene="map"
                     /locus_tag="RH033_000493"
     CDS             complement(31183..31977)
                     /gene="map"
                     /locus_tag="RH033_000493"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDV2989732.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32345..33070
                     /gene="rpsB"
                     /locus_tag="RH033_000494"
     CDS             32345..33070
                     /gene="rpsB"
                     /locus_tag="RH033_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDV2989733.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33328..34179
                     /gene="tsf"
                     /locus_tag="RH033_000495"
     CDS             33328..34179
                     /gene="tsf"
                     /locus_tag="RH033_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDV2989734.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            34326..35051
                     /gene="pyrH"
                     /locus_tag="RH033_000496"
     CDS             34326..35051
                     /gene="pyrH"
                     /locus_tag="RH033_000496"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDV2989735.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            35343..35900
                     /gene="frr"
                     /locus_tag="RH033_000497"
     CDS             35343..35900
                     /gene="frr"
                     /locus_tag="RH033_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDV2989736.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35992..37188
                     /gene="ispC"
                     /locus_tag="RH033_000498"
     CDS             35992..37188
                     /gene="ispC"
                     /locus_tag="RH033_000498"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDV2989737.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            37377..38135
                     /gene="ispU"
                     /locus_tag="RH033_000499"
                     /gene_synonym="uppS"
     CDS             37377..38135
                     /gene="ispU"
                     /locus_tag="RH033_000499"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HDV2989738.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            38148..39005
                     /gene="cdsA"
                     /locus_tag="RH033_000500"
     CDS             38148..39005
                     /gene="cdsA"
                     /locus_tag="RH033_000500"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDV2989739.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLVTAAVISWGYGMWVNLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            39017..40369
                     /gene="rseP"
                     /locus_tag="RH033_000501"
     CDS             39017..40369
                     /gene="rseP"
                     /locus_tag="RH033_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HDV2989740.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGKGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            40399..42831
                     /gene="bamA"
                     /locus_tag="RH033_000502"
     CDS             40399..42831
                     /gene="bamA"
                     /locus_tag="RH033_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HDV2989741.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42953..43438
                     /gene="skp"
                     /locus_tag="RH033_000503"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42953..43438
                     /gene="skp"
                     /locus_tag="RH033_000503"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HDV2989742.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43442..44467
                     /gene="lpxD"
                     /locus_tag="RH033_000504"
     CDS             43442..44467
                     /gene="lpxD"
                     /locus_tag="RH033_000504"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HDV2989743.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44572..45027
                     /gene="fabZ"
                     /locus_tag="RH033_000505"
     CDS             44572..45027
                     /gene="fabZ"
                     /locus_tag="RH033_000505"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDV2989744.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            45031..45819
                     /gene="lpxA"
                     /locus_tag="RH033_000506"
     CDS             45031..45819
                     /gene="lpxA"
                     /locus_tag="RH033_000506"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HDV2989745.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45819..46967
                     /gene="lpxB"
                     /locus_tag="RH033_000507"
     CDS             45819..46967
                     /gene="lpxB"
                     /locus_tag="RH033_000507"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HDV2989746.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAAVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46964..47560
                     /gene="rnhB"
                     /locus_tag="RH033_000508"
     CDS             46964..47560
                     /gene="rnhB"
                     /locus_tag="RH033_000508"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDV2989747.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPSMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47597..51079
                     /gene="dnaE"
                     /locus_tag="RH033_000509"
     CDS             47597..51079
                     /gene="dnaE"
                     /locus_tag="RH033_000509"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDV2989748.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGTAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLDTELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            51092..52051
                     /gene="accA"
                     /locus_tag="RH033_000510"
     CDS             51092..52051
                     /gene="accA"
                     /locus_tag="RH033_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDV2989749.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            52149..54290
                     /gene="ldcC"
                     /locus_tag="RH033_000511"
     CDS             52149..54290
                     /gene="ldcC"
                     /locus_tag="RH033_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HDV2989750.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPFGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFSDADANHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTEESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            54347..54736
                     /locus_tag="RH033_000512"
     CDS             54347..54736
                     /locus_tag="RH033_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDV2989751.1"
                     /translation="MLGLKQVHHIAIIATDYAMSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54801..56114
                     /gene="tilS"
                     /locus_tag="RH033_000513"
     CDS             54801..56114
                     /gene="tilS"
                     /locus_tag="RH033_000513"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDV2989752.1"
                     /translation="MTLTLNRQLLSSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVKHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTQQGTLKIAPMVAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETILPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAVGENGVSFVWQKTLSQVKAG"
     gene            complement(56148..56408)
                     /gene="rof"
                     /locus_tag="RH033_000514"
     CDS             complement(56148..56408)
                     /gene="rof"
                     /locus_tag="RH033_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HDV2989753.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGATRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(56395..56595)
                     /locus_tag="RH033_000515"
     CDS             complement(56395..56595)
                     /locus_tag="RH033_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HDV2989754.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56761..57306
                     /locus_tag="RH033_000516"
     CDS             56761..57306
                     /locus_tag="RH033_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HDV2989755.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFAYNSRAAQIWWQQNQSKCAQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTVWQQPS"
     gene            57303..57725
                     /gene="arfB"
                     /locus_tag="RH033_000517"
     CDS             57303..57725
                     /gene="arfB"
                     /locus_tag="RH033_000517"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDV2989756.1"
                     /translation="MIVISRHVAIPDGEFEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57739..58449
                     /gene="nlpE"
                     /locus_tag="RH033_000518"
                     /gene_synonym="cutF"
     CDS             57739..58449
                     /gene="nlpE"
                     /locus_tag="RH033_000518"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591565.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HDV2989757.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGNSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGL"
     gene            complement(58605..59429)
                     /locus_tag="RH033_000519"
     CDS             complement(58605..59429)
                     /locus_tag="RH033_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HDV2989758.1"
                     /translation="MDKPKAYCRLLLPCFLLLSACTVDISQPDSSATVVNAEAKTWAV
                     KFQHQSSFTEQSIKEITEPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAIKHSDKLFVLRVPDLTPQQATEITAFTNKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRSGDVSAFKPETQLQYIGHLKPGIYIKASRFVGLTQ"
     gene            complement(59482..61200)
                     /gene="proS"
                     /locus_tag="RH033_000520"
     CDS             complement(59482..61200)
                     /gene="proS"
                     /locus_tag="RH033_000520"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDV2989759.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVLMPVVQPSELWQESGRWEQYGPELLRIADRGDR
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVDYL
                     VKQIKG"
     gene            complement(61311..62018)
                     /gene="tsaA"
                     /locus_tag="RH033_000521"
     CDS             complement(61311..62018)
                     /gene="tsaA"
                     /locus_tag="RH033_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HDV2989760.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVCFTAEVEKQLLTLKKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(62015..62419)
                     /gene="rcsF"
                     /locus_tag="RH033_000522"
     CDS             complement(62015..62419)
                     /gene="rcsF"
                     /locus_tag="RH033_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HDV2989761.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62537..63352)
                     /gene="metQ"
                     /locus_tag="RH033_000523"
     CDS             complement(62537..63352)
                     /gene="metQ"
                     /locus_tag="RH033_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HDV2989762.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKESPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(63392..64045)
                     /gene="metI"
                     /locus_tag="RH033_000524"
     CDS             complement(63392..64045)
                     /gene="metI"
                     /locus_tag="RH033_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HDV2989763.1"
                     /translation="MSEPMMWLLVRGVWETLTMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(64038..65069)
                     /gene="metN"
                     /locus_tag="RH033_000525"
     CDS             complement(64038..65069)
                     /gene="metN"
                     /locus_tag="RH033_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HDV2989764.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKTRRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAITWLQEHH
                     VKVEVLGYV"
     gene            65257..65829
                     /gene="gmhB"
                     /locus_tag="RH033_000526"
     CDS             65257..65829
                     /gene="gmhB"
                     /locus_tag="RH033_000526"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HDV2989765.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     VTPEAENAADWVLNSLADLPQAIKKQQKPA"
     gene            66195..>66457
                     /locus_tag="RH033_000527"
     rRNA            66195..>66457
                     /locus_tag="RH033_000527"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT        15831 a        16413 c        18226 g        15987 t
ORIGIN      
        1 ttaaagcctt ttgcctgtag agcttcaact aatgataagc catcaggaaa tagcaattcc
       61 ggcgcaattt caaacagtgg ccacagcata aagccgcgat ttttcatatc gtagtgcgga
      121 acggtcagac gttcggtatt tattacttca ttaccaaaca gcatgatgtc gagatccagc
      181 gtgcgcggtc cccagcgttc agctttacgg acgcgacctt gctgcaattc gatacgctgt
      241 gtgtgattga gtagctcttc aggggcaaga gtggttttca gcgcgacggc tgcatttaag
      301 taatcgggtt gatcttgcgg ccccagcggt ggggtgcggt aaaacgaaga aacagcaaga
      361 atgcggcttt ctggaatatc gcctaatgct ttcagggcag cattgacctg ctccagcgga
      421 gaggccagat tgctgccgat ggcaatatac gccactgtca tgcggtaccc tcacgacgcg
      481 gtgcgcgttt gcgtggacga cgagtacggc gacgcggtga cggttcttca tccagttcgt
      541 taagcatccc tttttggtct ggtggcgcgg aaacctggaa ctcaccccac catttcacca
      601 gacgttgcag ttcagcgtta cgctcaactt cggctcgtaa ggccaacagg tcgtatgccg
      661 cacggaactt aggatgctcc agcagtttcc aggcgcgttt accctgacgt cgggacatac
      721 gcaactgcaa ctgccagata tcgcgggtta acgtcgtcag acgtttcggg atcgccagtg
      781 aacgacaggc ttcgtccagc acgtcgttca tcgccagcgc gaaagcgtcg tgataggtca
      841 ggccgctttc ctgggcgatc ttctgtgccg tctccagcag tgggtaccag aacatggcgg
      901 caaacaggaa cgccgggttc acgcgcatat cgttatggat acgcgtatcg gtattcttca
      961 gcacctgttc aatgatccgc tccatcgggc tgtcgccatt ttccgtgaag tagcgggtaa
     1021 tggtcgggaa cagcggctgg aacaggtgat attcacacag cagcttatag gtttcgtaac
     1081 cgtagcccgc ttgcagcagt ttcaaagatt cttcaaacag acgtgccggt ggaatatcgt
     1141 tcagcagggt agccaggcga gggatcggtt ctgcggtttc aggactgata cgcataccca
     1201 atttggcggc aaaacgtacc gcgcgcagca tccgtaccgg atcttcacgg tagcgcgttt
     1261 ccgggttacc aatcagacgg ataacgccgt ccttcagatc cttcatgccg ccaacgtaat
     1321 cacggacggt aaaatctgcc acgctgtaat acaggctgtt gatagtgaaa tcgcggcgct
     1381 gggcgtcttc ttcgatggag ccgaaaatgt tgtcgcgcag caacatgccg ttttgcccgc
     1441 gttgggaggt cgtgcggtcg ctgacgttac cttcgtgatg tccacggaag gtcgcaactt
     1501 cgataatctc cgggccaaac atcacatgag ccagacggaa acggcgaccc accaggcggc
     1561 agttacggaa cagtttacgt acctgttcag gcgtggcgtt agtggttacg tcaaaatctt
     1621 tcggcttttt gccaagtaac aggtcgcgca cgccaccgcc aaccagccag gcttcgtatc
     1681 ccgctttatt gagcctgtac attaccttca gggcattttc actgatatct ttgcgggaaa
     1741 tagcatgctg ctcacgcggg atcaccgtca cctgtggacg ggcgactgcc tgttcagcct
     1801 cgctttcctc gcggcttagc accttgcggc aaaaattagc gactcgggta aaaatagtac
     1861 acctcggtag tgtcaaacat cattcaggac aaaaaaatag cggctaatca tagctcagca
     1921 tgacgcattt gagaatgttg aatttacaat tgccgactca ggcacggcgg taagccgcca
     1981 gtttttgacg gctgactgaa ggatttgctc gacgctgaaa tcctgccagt gtgcttctgc
     2041 ctgctgaccc agaaattgaa gtgccgcgat tagtaccggg cgcggatcgc ctttcggcaa
     2101 cgcaggcgca tgattctgct tggaaagttt agcgccttgt ggattaagcg ccagcggaag
     2161 atgaatgtaa tctggcactt gccagccaaa aagctggtac agcgagattt gccgcactgt
     2221 cggttcgatc aaatctgccc cgcggacaat ttcgctcacg ccctggaagt gatcatccac
     2281 caccaccgcc aggttatagg caaacaaacc atcacggcga tgaataataa aatcttcccg
     2341 cgccagtttt tcgtcggcgt gaataatgcc gcgcagctgg tcagtaaatt gagtaaccgg
     2401 atgctgctgg cggatacgca ctgcggcgtt gtctggtcca tgatgcaatt cccggcaatg
     2461 accgtcgtaa ataccgccaa tgctttgaat acgcgcgcgc gtgcaggtgc agtaataact
     2521 tagtccttgc tcatgtaacc aggcgagtgc ttcgcgatag gcgtcgtgac gttgcgattg
     2581 ccagagaacg tcgccgtccc agtgcagacc gtaatgttcc agctggcgca ggatagtctc
     2641 tgcggcaccg ggaacttcac gaggcgggtc gatatcttct atgcgtacca gccagcgacc
     2701 gtgccgggcg cgagcctgta aatagctgcc aagcgcggca atcagagagc caaaatgaag
     2761 ttcgccggaa ggagagggag caaagcggcc aatatagtgt gtgtctgtca tctcgttgaa
     2821 caaaaaataa ggcgggagca tttcccgcct gtggtaaacg tgatggaacg gctgtaatta
     2881 gccagccatt tgtttttcgc gaatttcagc cagcgttttg cagtcgatgc atagatcggc
     2941 tgtcgggcgc gcttccagac ggcgaatacc aatttcaaca ccgcaggatt cgcagtagcc
     3001 gaaatcttcg tcttccactt ttttcagcgt cttctcgatc tttttgatca gcttacgctc
     3061 gcgatcgcgg ttacgcagtt cgaggctgaa ctcttcttcc tgggctgcac ggtctaccgg
     3121 gtccgggaag ttggctgctt catcctgcat atgtgtaacg gtgcgatcga cttcatccct
     3181 gagttgatta cgccatgctt ccagaatacg acggaagtgc gccagctggg cttcattcat
     3241 atactcttcg cccggcttct cctgatatgg ttccacccca gcgatggcga gaatactcag
     3301 ggacgatgtt ttacggtttt gcccttcttg catgttgctt ctccttaaca cgcactatcg
     3361 atccccatgt tcgggggaaa aatgaggccg ctataaatag cagatgcttt tccggatagc
     3421 aattatctaa acgtaacact tgacaactgt gtgaggaaaa gcgtatttgc gcacgcgacc
     3481 agaatgtaaa ttaaccagtt acatacttta ctacaatgta accggcagtg attttttaag
     3541 agccatgcct tcagcagaaa gttccgcttt gtaagccaga atttctaccc ccctctgttg
     3601 agcttctgac aatagttgcg cgtatttctc atcgatgtgg cgcgcgggtg aaaaccgtgt
     3661 aatggctgaa tgcagcacgg cgaaaaagat aaccgcacgc tggccttcag ccgctacgct
     3721 catcaactcc cgaaggtgtt tctgacctcg ttcagtgacc gcatcgggaa aatatccctg
     3781 ttcgttctcc gctaacgtaa ccgatttcac ttcaatatag cagtctggac gcgaatccgc
     3841 ctgcaacata aagtcaatac ggctgcgttc ggcgccgtat tttacttcgc ttttcagcga
     3901 gctatagcct gacagctctg aaattgattc attaaggaca gcctctttcg tcaacctgtt
     3961 agcccaaagc gtgttgacgc aaataaatgc gccgctctgg ctttgagtca attcccaggt
     4021 gtgtgggtat ttccgtttgg tgttgtctga agtcgaatac cagacggtat cgccaggcgt
     4081 tgcacaaccg gtcatcgcac ccgtattcgg gcagtgtagc gttaattcgc gaccatcggg
     4141 tgtgatcaca tcggctaaaa aacgtttgta acgctgaatt agcgtcgcgc gctgtagagg
     4201 gggagaaaat tccattacga caatccttgt tattgcgtta gcgcccagcg ttttagcggc
     4261 gtgtagcgtg tgcgtccacg ggcaaacgag gaggcgtaaa gggtgaactc cgtcaccgta
     4321 tacgaccagt taaaacctgg cggcgggatt gtcaccgcct cgctggcgtc gcgcaataag
     4381 gtaatatgtg gatgaaacgg acgattgctt tgaaaacaac cgctgcgggc agcctgtgaa
     4441 cggagcatat tcgccagctg gattaagccg cgtggtggct gacgcatccc taaccacacc
     4501 acacgcgaac gcagccattg accggcgtca tcaagcgtga gtgtgaaacc cggttgacga
     4561 atccgcccgg ctaaaagaga aagcgccttc tctttctcgg cgctcacttc gcctaaaaat
     4621 gccagagtca gatgcaaatt atcggcggcg accggacgtc ccgcgtcagg tgggaagtgt
     4681 gtggcgcgcc agtggataat ctgttcgcgg atttctgcag gtaagtcgat agcaaagaac
     4741 agccgttgcg gttcagacat gtgaggcact cggttatgaa ttaccgcgat gctacaatgt
     4801 ggcgcgaaga atgttaaccc tctggagcgt tttgtgtcgt cgttgcccgt tgctgccgtc
     4861 ttacctgagt tacttgctgc tctcgattgt gcatcgcagg tgttattaag tgcgccgacc
     4921 ggggccggga aatcaacctg gctgccgctg caactgctgg cgcatcccgg cattaacggg
     4981 aaaattatcc tgctggagcc gcgtcgtttg gcggcgcgta acgttgcgca acgactggca
     5041 gagctgctta acgaaaagcc aggcgatacc gttggctacc ggatgcgtgc gcaaaactgc
     5101 gtcgggccga atacccgcct ggaagtggtt accgaaggcg tgctgacgcg catgatccag
     5161 cgtgacccgg aactgagcgg tgttggattg gtgatcctcg atgagtttca tgagcgcagc
     5221 ttgcaggcgg atttggcgtt ggcgctgtta ctcgatgtgc aacaaggtct gcgtgatgac
     5281 cttaaactgc tgattatgtc ggctacgctg gacaacgaca gcttgcagca aatgctgcca
     5341 gaagcgcccg tcatcatctc agaagggcgc tcgtttccgg ttgaacgccg ttatttacca
     5401 ctgcccacgc atcagcgttt tgacgaagcc gttgcggtag ccaccgccga aatactgcgt
     5461 caggaaagcg gatcattact gttattttta cctggcgtcg gagaaattca gcgtgtgcag
     5521 gaacaactgg cttcgcgcat cggcagtgat gtgttgctct gcccgctgta tggcgcgttg
     5581 tcgctgaacg ctcagcgaaa agcgatcctc ccggcaccgc aagggatgcg caaagtggtg
     5641 ctggcgacca atattgctga aaccagttta accattgaag gtattcgtct ggtggtggat
     5701 tgtgcccagg agcgtgtggc gcgttttgat ccgcgcacgg ggcttacgcg actgattact
     5761 caacgcgtta gccaggcatc catgacgcag cgtgccgggc gcgccggtcg tctggagccg
     5821 ggtatctgcc tgcatttaat cgccaaagaa caagcagaac gcgctgcggc gcaaagtgaa
     5881 ccggaaatat tacaaagcga tctttccggt ttgctgatgg aattactgca atggggatgc
     5941 agcgatacgg cgcagatgag ctggctggat caaccgccag cagtgaattt acaggccgcg
     6001 aaacgcctgt tacggatgct gggggggctg gatggcgaac ggctaagcgc gcaagggcaa
     6061 aaaatggcgg cgctgggtaa cgatccgcgt ttagcggcaa tgctggtgag cgcgaagagc
     6121 gacgacgaag ctgctaccgc ggcaaaaatt gccgccattc tcgaagagcc gccacggatg
     6181 ggcaatagtg acctgggcgt ggcgttttcg cgcaatcaac cagcctggca gcaacgtagt
     6241 cagcaactgt taaaacgctt aaacgtacgc ggcggtgagg cagacagttc gcttatcgcg
     6301 ccgctacttg ccggggcgtt tgccgatcgc attgctcgcc gccgtgggca agatggacgc
     6361 tatcaactgg caaacggcat gggagcgatg ctcgatgccg acgacgcgct aagtcgccac
     6421 gaatggttga tcgcaccgtt attattgcag ggcagcgcct cgccggatgc gcggatttta
     6481 ctggcgctac cggtcgatat tgatgaatta gtacaacgct gtccgcagct ggtgcagcaa
     6541 tctgacaccg tggaatggga tgacgcgcaa ggtacgctga aagcctggcg tcggctgcaa
     6601 atcggtcagt tgacggtgaa agtgcagcca ctggcgaaac cgtcagaaga cgagttgcat
     6661 caggcgatgc ttaacggcat ccgcgataaa ggtttaagcg tgctcaactg gacggcggaa
     6721 gcggaacagc tacgcttgcg tttgttatgc gccgcaaagt ggttgccgga atatgactgg
     6781 ccagcggttg atgatgaaag tttattggcg gcgctggaaa cgtggctgct gccacatatg
     6841 actggcgtac attcactacg cggcctgaaa tcactcgaca tttatcaggc actacgcgga
     6901 ttacttgatt ggggaatgca gcaacgtctg gatagtgaat tgcctgcgca ttacactgtg
     6961 ccgacgggaa gccggatcgc cattcgttat catgaagata atccgcccgc gctggcggtg
     7021 agaatgcagg agatgtttgg tgaggccacc aatccgacga tcgctcaggg gcgcgtgccg
     7081 ctggtgctgg agttgctttc ccctgcccaa aggccgctgc aaatcacgcg tgatttgggg
     7141 gccttctgga aaggagcgta ccgtgaggtg caaaaagaga tgaaagggcg ttatcccaaa
     7201 catgtctggc cggacgaccc ggcaaacaca gcaccaacgc gacgaacgaa aaagtattcg
     7261 tgaagatcaa agtaaatttg agagatatct tcttctgtct tgcaacagaa gaacagaaaa
     7321 tcgggctttt gcgcctgaat attgcggaga aaaagcatgg ccgggaatga ccgcgagcca
     7381 attggacgca aagggaaacc gacgcgtccg gtcaaacaaa aggtaagccg tcgtcgttac
     7441 gaagatgacg atgattacga cgattatgat gactatgagg atgaagaacc gatgccgcgc
     7501 aaaggtaagg gcaaaggcaa agggcgtaag cctcgtggca aacgcggctg gctatggctg
     7561 ctgctaaaac tggctatcgt ttttgccgtg ctgatcgcca tttacggcgt ttatctcgat
     7621 caaaaaattc gcagccgtat tgatggtaag gtctggcaac tgcccgcggc agtttatggc
     7681 cggatggtca accttgagcc agacatgacc atcagcaaga acgagatggt gaagctgctg
     7741 gaggcgaccc agtatcgtca ggtgtcgaaa atgacccgtc ctggcgaatt taccgtgcag
     7801 gccaacagca ttgagatgat ccgccgtccg tttgatttcc cggacagtaa agaagggcag
     7861 gtgcgtgcac gtctgacctt tgatggcgat catctggcga cgatcgtcaa tatggagaac
     7921 aaccgtcagt ttggtttctt ccgtcttgat ccgcgcctga tcaccatgat ctcctcgcca
     7981 aacggtgagc aacgtctgtt tgtgccgcgc agtggtttcc ctgatttgct ggtggatact
     8041 ttgctggcga cagaagaccg tcatttttac gagcatgatg ggatcagtct ctactccatc
     8101 ggacgtgcgg tgctggcaaa cctgaccgcc ggacgcacgg tgcagggtgc gagtacgctg
     8161 acgcaacagc tggtgaaaaa cctgttcctc tccagcgagc gctcttactg gcgtaaagcg
     8221 aacgaagctt acatcgcgct gatcatggac gcgcgttaca gcaaagaccg tattcttgag
     8281 ctgtatatga acgaggtgta tctcggtcag agcggcgata acgaaatccg cggcttcccg
     8341 ctggcgagct tgtattactt tggtcgcccg gtagaagagc taagcctcga tcagcaggcg
     8401 ctgttagtcg gtatggtgaa aggggcgtcc atctacaacc cgtggcgtaa cccgaaactg
     8461 gcgctggagc gacgtaatct ggtgctgcgt ctgctgcaac agcaacagat tattgatcaa
     8521 gaactctatg acatgttgag tgcacgtccg ctgggtgttc agccgcgcgg tggggtgatc
     8581 tctcctcagc cagcctttat gcaactggtg cgtcaggagt tgcaggcaaa actgggcgat
     8641 aaggtaaaag atctctccgg cgtgaagatc ttcactacct ttgactcggt ggcccaggac
     8701 gcggcagaaa aagccgccgt ggaaggcatt ccggcactga agaaacagcg taagctgagc
     8761 gatcttgaaa ccgcgattgt ggtcgtcgac cgctttagtg gtgaagttcg tgcgatggtt
     8821 ggtggttctg agccgcagtt tgcgggctac aaccgtgcga tgcaggcgcg tcgttcgatt
     8881 ggttcccttg caaaaccagc gacttatctg acggccttaa gccagccgaa aatctatcgc
     8941 ctgaacacgt ggattgcgga tgcgccaatc gcgctacgtc agccgaatgg ccaggtctgg
     9001 tcaccgcaga acgatgaccg tcgttatagc gaaagcggca gagtgatgct ggtggatgcg
     9061 ttgacccgtt cgatgaacgt gccgacggtg aatctgggga tggcgctggg gctgcctgcg
     9121 gttacggaga cctggattaa actgggcgta ccgaaagatc agctgcatcc ggttccggcg
     9181 atgctgctgg gggcgttgaa cttaacgcca atcgaagtgg cgcaggcatt ccagactatc
     9241 gccagcggcg gtaaccgtgc accgctttct gcgctgcgtt cggtaattgc ggaagatggc
     9301 aaagtgctgt atcagagctt cccgcaggcg gaacgcgctg ttccggcgca ggcggcgtat
     9361 ctgacactat ggaccatgca gcaggtggta caacgtggta cggggcgtca gcttggggcg
     9421 aaatacccga acctgcatct ggcagggaaa acagggacta ccaacaataa cgtagatacc
     9481 tggtttgcgg gcattgacgg cagtacggtg accatcacct gggtaggccg tgataacaac
     9541 caaccgacca aattgtatgg agccagcggg gcaatgtcga tttatcagcg ttatctggct
     9601 aaccagacgc caacaccgct gaatcttgtt ccgccagaag atattgcaga tatgggcgtg
     9661 gactacgacg gcaactttgt ttgcagcggt ggcatgcgtg tcttgccggt ctggaccagc
     9721 gatccgcaat cgctgtgcca gcagagtgag atgcagcagc agccgtcagg caatccgttt
     9781 gatcagtctt ctcagccgca gcaacagccg caacagcaac ctgctcagca agagcagaaa
     9841 gacagcgacg gtgtagccgg ttggatcaag gatatgtttg gtagtaatta acgtctaagc
     9901 gtgacatacc ggatggcgag tagccatccg gtaaaataac atcccaccca aagtattaac
     9961 cctttctttt catctggttg tttattaacc cttcaggaat gcgcatattg cgtactgctt
    10021 gcgaacccgc cagcgtttcg aatattatct catctttata ataatcattc tcgtttacgt
    10081 tatcattcac tttacatcag agatatacca atggcgcgtt ccaaaactgc tcagccaaaa
    10141 cactcactgc gtaaaatcgc agttgtagta gccacagcgg ttagcggcat gtctgtttat
    10201 gcacaggcag cggttgaacc gaaagaagac actatcaccg ttaccgctgc acctgcgccg
    10261 caagaaagcg catgggggcc tgctgcaact attgcggcga agcactctgc tactgcgact
    10321 aaaacggata caccaattga aaaaacgccg cagtctattt cggttgtgac aaatgaagag
    10381 atacagatgc atcaatttca gtctgtaaaa gaagcattag gttatacacc tggcgttacg
    10441 gttagcagtc gtggtgcttc taatacatat gattttgtta tcattcgtgg tttctcatct
    10501 gttggtctga atcaaaataa ctaccttgat ggactaaaac ttcagggcaa cttttataac
    10561 gatgctgtga ttgatcctta catgctcgag agggttgaac tgatgcgcgg gccgacgtct
    10621 gttctctatg ggaaaagtaa tcctggtggg attatttcta tggtgagtaa gcgtccgaca
    10681 actgaacccc tgaaagaaat tcagtttaaa atggggacgg ataatctgtt tcagaccgga
    10741 ttcgatttca gtgatgcgct ggatgataac ggtgagttct cctaccgttt gactggcctc
    10801 gcacgctcaa caaacgaaca gcagaaaaac tctgaatctc agcgttatac cattgctcca
    10861 tcattctcat ggcgtccaga cgacaaaacc aattttactt tcctgtccta ttttcagaac
    10921 gaaccagaaa cgggttatta cggttggttg ccgaaagagg ggaccgttga gccattgcct
    10981 aatggtaagc gtctaccgac tgacttcaat gaaggtgcat cgaataatac atactcccgt
    11041 aaccagaaaa tggtgggata tagttttgaa catggtttta atgacacctt caccgtgcgt
    11101 cagaatctgc gtttcagtga aatgaaaacc tcacagaaaa gtgtttatgg cacagggatt
    11161 gccagcgatg gtcataccct aaaccgcggg acagtggtgg ataatgagcg tctgcaaaac
    11221 tttagcgttg atacccaact tgaaagtaaa tttgctacag gtgaagtcga gcatactttg
    11281 ctgacagggg tagacttcat gcgtatgcgc aatgatatca atgccagctt tggatctgca
    11341 ccatccatcg atctttataa caaatatcat cctgaatact ttgcatttgg taacgcagag
    11401 ccataccaaa tgaatgaaag caaacaaaca ggtatttatg ttcaggatca ggcggaatgg
    11461 aataaatggg tattcactct ggggggacgc tacgattggt ctaagcaagc gaccactgtt
    11521 cgtgaaaact cttatacgcc gactgaaggt tatattgagc gtaatgatca tcagttcacc
    11581 tggcgcggtg gtgtaaacta cttattcgat aatggtattt caccttactt tagttatagc
    11641 cagtcctttg aaccgagtgc tttcgatctg tggagcaacc cgcgcgtttc ctataagcca
    11701 tcgaaaggtg aacagtatga agctggcgta aaatatgttc cgaatgatat gccggtcgtt
    11761 gttacgggcg cagtctatca attgacgaaa acaaataacc tgacagcaga cccaacaaac
    11821 ccgttagcgc aagtcccagc aggtgagatt cgcgctcgtg gtgtggaact tgaagcaaaa
    11881 gcggcgttaa atgccaatat taacttgacg gcttcttata cctacacaga tgcggaatac
    11941 accaaagaca ccaatctcaa aggtaaaact ccagaacaag taccggagca tatggcatct
    12001 ctctgggggg attatacctt caatgaaggg ccgctttctg gtttaacatt gggaacaggt
    12061 ggtcgtttta ttggttccag ctatggtgat ccggcgaaca cttttaaagt gggtagcgca
    12121 gctgtaatgg atgctgttgt aaaatatgat ctggcacgct ttggtatggc gggatccagc
    12181 cttgctgtga acgtcaacaa tttgctcgat cgtgagtatg ttgccagttg cttccagacc
    12241 tatggctgct tctggggcgc agaacgtcag gtcgttgcaa ccgcaacctt ccgtttctaa
    12301 ttttttcttt ggggcacgga tttccgtgcc cgcttcacaa gttggctgtt atgcaggaat
    12361 acacgaatca ttccgatacc acttttgcac tgcgtaatat ctcctttcgt gtgccagggc
    12421 gcacgctttt gcatccgctg tcgttaacct ttcctgccgg gaaagtgact ggtttgattg
    12481 gtcacaacgg ttctggtaaa tccactctgc tcaaaatgct tggccgtcat cagccaccgt
    12541 cggaaggcga aattcttctt gatgcccagc cgctggaaag ctggagcagc aaagcgttcg
    12601 cccgcaaagt ggcttatttg ccgcagcagc ttcctccggc agaagggatg accgtgcgtg
    12661 aactggtggc gattggtcgt tacccgtggc atggcgcact ggggcgcttt ggtgcggcag
    12721 atcgcgaaaa ggtcgaagag gcgatttcgc tggttggctt aaaaccgctg gcgcatcggc
    12781 tggtcgatag tctctctggc ggcgaacgtc agcgggcgtg gatcgctatg ctggtggcgc
    12841 aggatagccg ttgtctgttg ctcgacgaac cgacctcggc gctggatatc gcccaccagg
    12901 ttgatgtgct ggcgctggtg catcgtttaa gtcaggaacg tggtctgacg gtcattgccg
    12961 tgctgcacga tattaatatg gctgcgcgct actgtgatta tctggtcgcc ctgcgcggtg
    13021 gtgaaatgat tgctcaggga acgcctgcgg aaattatgcg cggcgaaacc ctcgaaatga
    13081 tttatggcat cccgatgggt attctgccgc atccggcggg tgctgcacct gtgagttttg
    13141 tttattgatg agcggcttac ctcttatttc gcgccgccga ctgttaacgg cgatggcgct
    13201 ttctccgttg ttatggcaga tgaataccgc ccacgcggcg attattgatc ccaatcgtat
    13261 tgtggcgctg gagtggttgc cggtggaatt actgctggcg ctcggcatcg tgccttacgg
    13321 cgtggcggac accatcaact atcgcctgtg ggtcagcgaa ccgccattgc cggactcagt
    13381 gattgacgtt ggtttgcgta cagaacccaa ccttgaactg ctgaccgaaa tgaaaccgtc
    13441 gtttatgatc tggtcggcag gatatggccc ttcaccagaa atgctggcac gtattgcgcc
    13501 ggggcgcgga tttaacttca gcgacggcaa acagccactg gcgatggcgc gtaaatcact
    13561 gacggaaatg gcagatttac ttaacctgca aagcgcagca gaaacgcatt tagcgcacta
    13621 tgaagacttt atccgcagca tgaaaccccg ctttgtgaag cgtggtgcgc gcccgttatt
    13681 gctgacgacg cttatcgatc cgcgccatat gttggtcttc ggtccaaaca gcttgttcca
    13741 ggaaattctc gatgagtacg gcatcccaaa tgcctggcaa ggggaaacca acttctgggg
    13801 cagtaccgcc gtcagtatcg atcgtctggc agcgtataaa gacgttgatg tgctctgttt
    13861 tgatcacgac aacagcaaag acatggatgc gctaatggca accccgctgt ggcaggccat
    13921 gccgtttgtc cgcgccggac gctttcagcg cgttccggcg gtctggttct atggtgcgac
    13981 gctttctgca atgcactttg tgcgtattct ggataacgcc atcggaggta aagcgtgagt
    14041 aaacgaattg cgctttttcc tgcgttattg ctggcgctgt tagtgattgt cgctacggca
    14101 ctgacctgga tgaacttctc gcaggcgctg ccgcgtagcc agtgggcgca ggccgcctgg
    14161 tcgccggata ttgacgtcat cgagcagatg atttttcact acagcttgtt gccgcgtctg
    14221 gcgatttctc tactggtagg cgcgggcctg gggctggtgg gcgtgctgtt tcagcaagtg
    14281 ctgcgtaacc cgctggcgga accgacgacg ttgggtgttg caacaggcgc gcaactgggg
    14341 attaccgtca ccacgctctg ggcgatccca ggtgctatgg cgagccagtt tgctgcgctg
    14401 gcaggggctt gcgttgttgg cttaattgtc tttggcgtcg cgtgggggaa acggctttcg
    14461 ccggtaacgc tgatcctcgc ggggctggta gtgagccttt attgtggggc aatcaatcag
    14521 ttactggtta tcttccatca tgaccagttg caaagcatgt tcctgtggag caccggaacg
    14581 ctgacgcaaa ccgactgggg cggcgttgag cgtttatggc cgcagctgct gggcggcgtg
    14641 atgctgacgt tattgctact tcgcccgtta accctgatgg ggcttgatga tggcgtggcg
    14701 cgcaatctcg gtctggcctt gtcgctggcg cgtctggcgg cgctgtcgct ggcgatagtc
    14761 atcagtgcgc tgctggtgaa tgcagtgggg attatcggct ttatcgggtt gttcgcgccg
    14821 ctgctggcaa aaatgctggg ggcgcggcgt ctgctgccac ggttaatgct ggcgtcgctg
    14881 attggtgcgt taattctgtg gctttctgat caaatcatcc tctggctgac tcgcgtgtgg
    14941 atggaagttt ccaccggttc ggtcactgcg ttgatcggtg cgccgctgct actgtggctg
    15001 ctgccgcgtt tacgcagcat tagcgcgccg gatatgaagg tcaacgatcg tgtcgcggct
    15061 gaacgccaac atgtgctggc gtttgccctc gcgggcggcg tgctgctgtt gatggctgtg
    15121 gtggtagcgc tgtcgtttgg tcgtgatgcg cacggctgga cgtgggctag cggggcgttg
    15181 ctcgaggatt tgatgccctg gcgctggccg cgaattatgg cggcgctgtt tgcgggcgtc
    15241 atgctggcgg tggcgggctg tattattcag cgactgaccg gcaacccgat ggcaagcccg
    15301 gaagtgctgg ggattagctc cggcgcggcg tttggcgtgg tgttaatgct gtttctggtg
    15361 ccgggtaatg cctttggctg gctgttaccc gcgggtagtc tcggggcggc ggtgacgctg
    15421 ttgatcatta tgatcgccgc cggacgcggt ggattttctc cacaccgcat gttactggcg
    15481 gggatggcgt taagtaccgc gttcaccatg cttttgatga tgttgcaggc aagtggtgac
    15541 ccgcgaatgg cgcaagttct gacctggatt tccggttcga cctacaacgc gaccgatgcg
    15601 caggtctggc gcacgggaat tgtgatggta attttgctgg cgattacccc gctgtgccgc
    15661 cgctggctga ccattttacc gctgggaggt gataccgcac gagccgtggg aatggcgctg
    15721 acgccgacgc gaattgcgct gctgctatta gcggcttgcc tgacggcgac cgcgacgatg
    15781 actattggac cgttgagttt tgttggttta atggcaccgc atattgcgcg gatgatgggt
    15841 tttcgacgga cgatgccaca catcgtaatt tcggcgctgg tgggtggttt actgctggtg
    15901 ttcgctgact ggtgtgggcg gatggtattg tttccattcc agatcccggc ggggctgctg
    15961 tcaaccttta tcggcgcgcc atattttatc tatttgttga gaaagcagag ccgttaatag
    16021 aatgcctcgc ccggtgggcg aggcaaaatc atcacaactt cgcaaacacc cgacgtgcag
    16081 catcgatggt gttattgata tcttccatgc tgtgcgccac agacataaag cccgcttcaa
    16141 acgctgacgg tgccagataa acaccttcgt ccagcatcat atggaagaaa cgcttaaaac
    16201 gttccacgtc acaggccatc acatcctgat agcacgtcac ggactcggcg tcggtaaaga
    16261 aaataccgaa catgccgcca acgtggttta ccaccagcgg aattccggct tcttctgccg
    16321 cttcccgcag accttctgcc agacgtgttg tcagctcatc cagcgtctcg tgaacgcccg
    16381 gctgtgcgac ttcattcaga caggcgaaac cagcagccat cgcaatcggg ttaccggaaa
    16441 gcgtacccgc ctgatagacc ggaccggtcg gggccagcgc atccattaca tcacgacgac
    16501 caccgaatgc gcctaccggc attccaccgc cgatgatttt gccgaggcag gtgagatccg
    16561 gttccacacc gtaataatcc tgtgcgccag ctagcgctac gcggaaaccg gtcatcactt
    16621 catcgatgat cagcaacgcg ccaaattcgt cgcacagcgc gcgcagacct ggcaggaact
    16681 ctggcagcgg tggaacgcag ttcatattgc ctgccaccgg ctcgacgata atacaggcaa
    16741 tctcttgcgg gtattgctca aacgcggcgc gtacagaggc cagatcgtta taagtacagg
    16801 ttaaggtatg tttggcgaaa tctgccggaa cgcccggcga atttggctgg cctaacgtga
    16861 gtgcgccaga accggctttc accagcaggc agtcagcgtg accgtggtaa caaccttcaa
    16921 atttaataat tttgtcgcga ccggtaaaac cacgggccag gcggatggcg ctcatcgtcg
    16981 cttcagtgcc ggagttcacc atgcgcacca tatccatggt cgggaccagt tcggtcacca
    17041 gttgcgccat tttcacttcc atttcggttg gtgcaccaaa gcttaaacca cgctcggcgg
    17101 cttcaatcac ggcattgcgg atcgccggat ggttatggcc cagcaccatc ggcccccagg
    17161 aaccgacata atcgatatag gctttgccat caacatcgta cagataagcg ccgtccgctt
    17221 tttcgataaa cagtggagtg ccgcccacgc cagtaaaggc gcgaacaggg gagttcacac
    17281 cgccagggat cagctcgcgc gctgcgctgt aaagattttc agacttactc atggagggtt
    17341 cctgattcgt agaaaaagtg aatggctgct attctatgtt attcataaca agttaaatac
    17401 tcgtcaaaca tcaggctgct tgtactggtg caatcctgat ttcttagagt ataaaagttt
    17461 tgtgcatttg aaacattacg ctttgcaaag gattttcatg gaacgtgcga gtaaaatgcc
    17521 gtcatcttat ttgtatgacc aataagtgat cattggatga aaactgatac tccctcttta
    17581 gaaacaccgc aggccgcgcg cctgcgacgc agacaactga ttcgccaact tcttgagcgc
    17641 gataaaaccc cgttagccat tttgtttatg gcggctgtcg tcggcacgct tgtcgggctg
    17701 gctgcggttg cttttgacaa aggtgtcgcc tggttgcaga accaacgtat gggggcactg
    17761 gtacatactg ctgataatta tccgctactg ttaaccgtcg cttttctctg ttcggcggtg
    17821 ctggcgatgt tcggctactt tttggtgcgc aaatacgcgc cggaagcagg tggttcgggg
    17881 atcccggaaa ttgaaggggc gctggaagat caacgtcccg ttcgctggtg gcgtgtgttg
    17941 ccggtgaaat tctttggcgg gctgggaaca ctcggcggag gcatggtgct gggccgcgaa
    18001 gggccaaccg tacagatcgg cggcaatatc ggtcgcatgg tgcttgatat tttccgcctg
    18061 aaaggtgatg aagcgcgcca tacgctgctg gcaaccggtg ctgcggcggg gctggctgcg
    18121 gcctttaacg ccccgctggc gggtattttg tttattatcg aagagatgcg tccgcagttt
    18181 cgctatacgt taatttcgat taaagcggta tttattggcg tcattatgtc gaccattatg
    18241 taccggattt ttaatcatga agttgcgttg attgacgtcg gtaaactttc tgacgcgccg
    18301 cttaatacgc tgtggcttta tctgatcctc ggtattattt ttggcatttt cggccctatt
    18361 tttaataaat gggtgctggg gatgcaggat ttgctgcacc gtgtgcacgg cggcaatatt
    18421 accaaatggg tactgatggg cggtgcaatt ggcggtctgt gtggattgct ggggtttgtg
    18481 gcaccagcaa cgtcgggcgg cggttttaac ctgattccta tcgctaccgc ggggaatttc
    18541 agcatgggaa tgctggtgtt tatcttcgtc gcgcgggtca ttaccacctt actctgcttc
    18601 tcttccggcg cgccgggcgg tatttttgcc ccgatgctgg cgctgggtac tgtgctggga
    18661 accgctttcg gaatggttgc cgttgagctg tttccgcaat atcaccttga ggcggggaca
    18721 tttgctattg ccggaatggg ggcattactg gcggcatcta ttcgcgcgcc gttaacgggg
    18781 atcattctgg ttctggaaat gaccgataac taccagctca ttttgccaat gattattacc
    18841 ggtcttggcg caacactatt agcgcaattt accggcggga aaccgctata ctcggcgatt
    18901 cttgcgcgca cgctggcgaa acaggaagct gagcaactgg cgcgaagcaa ggccgcatca
    18961 gccagcgaga atacttgaac gaaataccag ggtattagat aatggcgatt attattgggt
    19021 tagaatttgc ccaattgccg atgtcgtttg gagcaaaata tgagtgatga cgtagcactg
    19081 ccgctggagt ttaccgacgc agcagccaac aaagttaaaa gcctgatcgc tgacgaagat
    19141 aacccgaatc tgaaattacg cgtgtatatc accggtggcg gttgcagcgg cttccagtat
    19201 ggtttcacct ttgatgatca ggtgaacgaa ggcgatatga ccatcgaaaa acagggcgtt
    19261 ggcctggtgg ttgatccgat gagcctgcaa tatctggtcg gcggttccgt tgactatacc
    19321 gaaggtctgg aaggttctcg tttcatcgtg accaacccga acgcgaaaag cacctgcggt
    19381 tgcggttctt cctttagcat ctaatctgtt gttcatgtgc cggataaact atccggcaca
    19441 tcttctctct tcaacgccca ttctcatcca gcgcaaacgt cggtagctta agatgccaac
    19501 gaatagccgc cagccgaatc aatagcgtca cgaccatgcc catcatactg gctgtttcca
    19561 gtggtacgta aaatgtgtaa taagccgtag cgtggacaat accgccgata atacaggctg
    19621 ttgcgtagat ttctgtacgt aaaatcatgg ggatctcgcg ggcaagtaca tcacgaatga
    19681 tcccgcctcc aacgccagta atgacgccca tacaaaccgc gattaacgga ccggcttccg
    19741 cattaaaggc tttattcacg ccaatgccga caaacaccgc cagaccaacg gcgtccagca
    19801 ccggcaacat ccattttggt aagcgtcttg gctggcgcac cagcacgatg gtcagcatgc
    19861 tggtgaccat tgcaacgacc agatcggtgg gatctttcac ccaaaatacc gggccgtgat
    19921 ccagcgccat gtcgcgaatt gttccgccgc ctactgcggt aaccacgccc aataccagaa
    19981 caccaaaagg gtccatacgc aattttccgg ctaacaaaac gccagagata gcaaataccg
    20041 ctgtgccgac tatatccagc caatagacga gcattgttca atccccactg agcacctgaa
    20101 aaggtcaggc attaatctac ctgtgaaagc gcattacaga gctgttgtgc agcgaggata
    20161 atacgtgggc ttgcacgttc aaaccagtca ctcgtgagag gaataacggg aattttgagc
    20221 tgttcacccc agtattgttt gattttagga atttggtccg gtccgcctgt aatgacaatc
    20281 gcctgtggcg cgcgtgctaa cacctgttcg cggctaactt gcggccaggg aacccggctg
    20341 tctttaaaga tgttttctcc gccacaaact tcgagtacct ggttttgaat cgactctttt
    20401 ccactagtaa atggcggatt aatgccgaat tgcagaaaaa cacgtttttt aggtttatca
    20461 gcatattgcg ctttcaattg cgcgtactgg tccagcaggg attgcgcggc ttgttcggcc
    20521 ttatccggtt gcggactcca ggggaccagt tgacgtaacg cattggcaat ttgttcaatg
    20581 cttgtcgcat cgacccacat cacttttatt cccagagaag ccagctggtc aacctgccgc
    20641 tcggcattac ctccacgcca ggcaatcacc agatcgggtt tcagcgccac aatgcgttcc
    20701 agattcatcc cctgccaggt ggaaacctgc tcaatttttt gcgcttgtgg aggatagtcg
    20761 gaatagctgc tgaccccaac cggcgtgatc ccggcggcaa aggcaagttc agtgttggcg
    20821 ggagaaagcg tgatgacgcg cggcgcggcg ttgagccaca gcggcgcgag aaaagacagg
    20881 gcgaccagcg ccctgaacag tgacttagcc atgtgccagt ttctgcacca gtgactcaac
    20941 catcaggctg gactgtttag cggcaacagc caggaactca tcgaagctaa gatgagactg
    21001 ttgatcggcc acgtcggaaa tggcgcgtac gacaacaaac gggacgttga aattgtggca
    21061 gacatgggcg attgccgtcg cttccatctc tacagcaatg gcctgtggga agttgtggcg
    21121 gattttcgcc agaccaacag aaccattgat gaaagcgtcg ccgctgacaa tcaggccacg
    21181 tacagcgttt agattcagtt cggcaatgca ggcctcagcg gcagcgatca gtttatcgtc
    21241 agctttaaag cctgccgggc agcctggtaa ctggccgtat tcataaccaa atgccgtgac
    21301 atccgcgtcg tgataacgcg cttcgtccga gacaacgata tcgcccactt tcagtgttgg
    21361 tgccaggcca ccggcagaac cggtgttaat aatcacatct ggcttgcagt gttccaacag
    21421 caaagtggca cccagcgccg cagcgacttt accgatgccc gatttcagaa gcgcaacctc
    21481 ggttccattc agttggccgg tatagatttc gcaaccgccg agactgatag tttgacggtt
    21541 ttcgattttg tcacgcagta gcgtaacttc ttcttccatt gcaccaatga tgccgatttt
    21601 catagattta ctcgcgataa gcccgatttg aaggcatagt ttaccatgcg cttacgggga
    21661 agcgtatttc tcacgcggga gaggacatgg cacagattga tttccgaaaa aaaataaact
    21721 ggcatcgtcg ttaccgttca ccgcagggcg ttaaaaccga acatgagatc ctacggatct
    21781 tcgagagcga tcgcgggcgt atcatcaact ctccggcaat tcgtcgtctg caacaaaaaa
    21841 cccaggtttt tccactggag cgcaatgccg ccgtgcgcac gcgtcttacc cactcgatgg
    21901 aagtccagca ggtggggcgc tacatcgcca aagaaatttt aagccgtctg aaagagctta
    21961 aattactgga agcatacggc ctggatgaac tgaccggccc ctttgaaagc attgttgaga
    22021 tgtcatgcct tatgcacgat atcggcaatc cgccgtttgg tcattttggc gaagcggcga
    22081 taaatgactg gtttcgccag cgtttgcacc cggaagatgc cgaaagccag cctctgactg
    22141 acgatcgctg cagcgtggca gcactacgtt tacgggacgg ggaagaaccg cttaacgagc
    22201 tgcggcgcaa aatacgtcag gacttatgtc attttgaggg gaatgcacaa ggcattcgtc
    22261 tggtgcatac attgatgcgg atgaatctca cctgggcaca ggttggcggt attttaaaat
    22321 atactcgtcc ggcgtggtgg cgtggtgaaa cgcctgagac acatcactat ttaatgaaaa
    22381 agccgggtta ttatctttct gaggaagcct atattgcccg gttgcgtaaa gaacttaatt
    22441 tggcgcttta cagtcgtttt ccattaacgt ggattatgga agctgccgac gacatctcct
    22501 attgtgtggc agaccttgaa gatgcggtag agaaaagaat atttaccgtt gagcagcttt
    22561 atcatcattt gcacgaagcg tggggccagc atgagaaagg ttcgctctat tcgctggtgg
    22621 ttgaaaatgc ctgggaaaaa tcacgctcaa atagtttaag ccgcagtacg gaagatcagt
    22681 tttttatgta tttacgggta aacaccctaa ataaactggt accctacgcg gcacaacgat
    22741 ttattgataa tctgcctgcg attttcgccg gaacgtttaa tcatgcatta ttggaagatg
    22801 ccagcgaatg cagtgatctt ctaaagctat ataaaaatgt cgctgtaaaa catgtattta
    22861 gccatccaga tgtcgagcag cttgaattgc agggctatcg ggtcattagc ggattattag
    22921 agatttatcg ccctttatta agcctgtcgt tatcagactt tactgaactg gtagaaaaag
    22981 aacgggtgaa acgtttccct attgaatcgc gcttattcaa caaactctcg acgcgccatc
    23041 ggctggccta tgtcgaggct gtcagtaaat taccgtcaga ttctcctgag tttccgctat
    23101 gggaatatta ttaccgttgc cgcctgctgc aggattatat cagcggtatg accgacctct
    23161 atgcgtggga tgaataccga cgtctgatgg ccgtagaaca ataaccaggc ttttgtaaag
    23221 acgaacaata aatttttacc ttttgcagaa actttagttc ggaacttcgc gctataaaac
    23281 gaatctgaag aacacagcaa ttttgcgtta tctgttaatc gagactgaaa tacatgaaaa
    23341 aaaccacatt agcactgagt gcactggctc tgagtttagg tttggcgtta tctccgctct
    23401 ctgcaacggc ggctgagact tcttcagcaa cgacagccca gcagatgcca agccttgcac
    23461 cgatgctcga aaaggtgatg ccttcagtgg tcagcattaa cgtagaaggt agcacaaccg
    23521 ttaatacgcc gcgtatgccg cgtaatttcc agcagttctt cggtgatgat tctccgttct
    23581 gccaggaagg ttctccgttc cagagctctc cgttctgcca ggggggccag ggcggtaatg
    23641 gtggcggcca gcaacagaaa ttcatggcgc tgggttccgg cgtcatcatt gatgccgata
    23701 aaggctatgt cgtcaccaac aaccacgttg ttgataacgc gacggtgatt aaagtccagc
    23761 tgagcgatgg ccgtaagttc gacgcgaaga tggttggcaa agatccgcgc tctgatatcg
    23821 cgctgatcca gatccagaac ccgaaaaatc tgaccgcaat taagatggcg gattctgatg
    23881 cactgcgcgt gggtgattac accgtagcaa ttggtaaccc gtttggtctg ggcgagacgg
    23941 taacttccgg gattgtctct gcgctggggc gtagcggcct gaatgctgaa aactacgaaa
    24001 acttcatcca gaccgatgca gcgatcaacc gtggtaactc cggtggtgcg ttggttaacc
    24061 tgaacggcga actgatcggt atcaacaccg cgatcctcgc accggacggc ggcaacatcg
    24121 gtatcggttt tgctatcccg agtaacatgg tgaaaaacct gacctcgcag atggtggaat
    24181 acggccaggt gaaacgcggt gagctgggta ttatggggac tgagctgaac tccgaactgg
    24241 cgaaagcgat gaaagttgac gcccagcgcg gtgctttcgt aagccaggtt ctgcctaact
    24301 cctccgctgc aaaagcgggc attaaagcgg gtgatgtgat cacctcactg aacggtaagc
    24361 caatcagcag ctttgccgcc ctgcgtgctc aggtgggtac tatgccggtg ggcagcaaac
    24421 tgaccctggg cttactgcgc gacggtaagc aggttaacgt gaacctggaa ctgcagcaga
    24481 gcagccagaa tcaggttgat tccagctcca tcttcaacgg cattgaaggc gctgagatga
    24541 gcaacaaagg caaagatcag ggcgtggtag tgaacaacgt gaaaacgggc actccggctg
    24601 cgcagatcgg cctgaaaaaa ggtgatgtga ttattggcgc gaaccagcag gcagtgaaaa
    24661 acatcgctga actgcgtaaa gttctcgaca gcaaaccgtc tgtgctggca ctgaacattc
    24721 agcgcggcga cagcaccatc tacctgttaa tgcagtaatc tccctcaacc ccttcctgaa
    24781 tacgggaagg ggttctcctt acaatctgtg aacttcgcca caactccata catcttcatc
    24841 atcctttggg catttgcaca atgccgtacg ttacgtactt ccttatgcta agccgtgcat
    24901 aacggaggac ttatggctgg ctggcatctt gataccaaaa tggcgcagga tatcgtagca
    24961 cgtaccatgc gcatcatcga taccaatatc aacgtaatgg atgcccgcgg gcgaattatc
    25021 ggcagcggcg atcgtgagcg tattggtgaa ttgcacgaag gagcattgct ggtactttca
    25081 cagggacgag tcgtcgatat cgatgacgcg gtggcacgtc atctgcacgg tgtgcggcag
    25141 gggattaatc taccgttacg gctggaaggt gaaattgtcg gcgtaattgg cctgactggt
    25201 gaaccagaga atctgcgtaa atatggcgaa ctggtctgca tgacggctga aatgatgctg
    25261 gaacagtcgc ggttgatgca cctgttggcg caggatagcc gtttgcggga agaactggtg
    25321 atgaacctca ttcaggctga ggagaatact cccgcactta ctgaatgggc gcaacggctg
    25381 gggatcgatc tcaatcaacc gcgagtggtg gctattgttg aggtcgacag cggtcagctt
    25441 ggcgtggaca gcgcaatggc ggagttacag caactgcaaa atgcgctgac tacgcccgag
    25501 cgtaataatc tggtggcgat tgtctcgcta accgaaatgg tggtgttgaa accggcgttg
    25561 aactcttttg ggcgctggga cgcagaagat catcgtaaga gagttgaaca actgattacc
    25621 cgcatgaaag agtatggcca gttgcgtttt cgcgtttcgc tgggcaacta ttttaccggt
    25681 cctggcagta ttgcccgatc ctatcgtacg gcgaaaacga cgatggtagt ggggaaacag
    25741 cggatgccag aaagtcgctg ctatttttat caggatctga tgttacctgt gttactcgac
    25801 agtttgcgtg gcgactggca ggccaacgaa ctggcgcgac cgctggcgcg gctgaaagcg
    25861 atggataata acggcttgct gcgacgtacg ctggcggcgt ggtttcgcca taacgtgcag
    25921 ccgctggcaa cgtcaaaggc gctgtttatt catcgtaata ccctggagta tcggcttaat
    25981 cgtatatcgg aactcacagg cttagatctg gggaattttg atgacaggtt attgttgtat
    26041 gtggcgttac aactggatga acagcgatag ggtaaaaata gtaaatgccg ggaaatatgc
    26101 tcgtcttaat tcaagttgca tgtacggcga ctcgaattat tcagcgtata ctttacccgg
    26161 ctggcagaat ggcaatatgc aaaattattt acgtgtcagc ttttctaaat ctgcttctat
    26221 ctcgctgatt ttattggtca ccactgactc caggtgacgc aagtcgtcga gaattttacg
    26281 cttaaggtca acttcgctac gatcacgctg gcagatttga tcaagctcat caatgatata
    26341 acgaagatta gggctgattt cctgaacttc tttatatccc tggccaatac catcggcgac
    26401 caccgttttg cgctgacgcg gatatttaaa cttcacactc ttggcgaaaa actcgccttt
    26461 atctttctga aaatagattt tcaaaatatc gttattggct tcctgccgga ggctgtaacg
    26521 atcaatttcc tcaggattgg taatgcccaa acttttcaga ttgtcgtaca tagttgtacc
    26581 tcaaaagagt cagtaattgt gcttatctta gcatttcgcc tggcggttta gctcgacgga
    26641 gatcgcagga agccttttct tccagacttt tatcatggcc taaaaatcaa aaaattacct
    26701 gctttattct gataagagaa ttcgctttct acaccagttc caggattgtc acttccgggc
    26761 ggcaattcag acgcaagcca tacaaacttc cgacaccccg agtcgtgtaa atttgtcgct
    26821 caccaaaggc atttaaaccg gcggcgtagc gtttatcttc tatgggcgca aaaggctcac
    26881 caaccaacgg tatgcgtaac tgcccgccgt gggtgtgacc acagagcatc agatcccact
    26941 tttcattacg catgacttct ttgctgtcgg gattatgcgc cagcaccagt cgtggcagat
    27001 ttgcttcgct ggcgggaggc ggtttgcatt gtccggtcca taaatcaccc gtgccaacca
    27061 gttcaaattg ctgcttttga gtggtgatta gcatggcttc gttaaataac accgtgatcc
    27121 ccgccgattt taatgcctcg ccaattaagc gatttttctc tgtaccaaca gggcgatcgt
    27181 ggttgccgta acaggcaaat gtcggcgcac attcggcaag ggaggagagt acgtcactaa
    27241 acgccgaaaa attcagctgc atatcaaata atacgtaatc gccgcccagc aatatcaaat
    27301 cgggcttttg tgctacgcca agagcaattg cgtcagaaat caggcttaaa ggaacaaaac
    27361 gagagtaatg gagatctgcc agaaaaagga ttttgaaagg agcagcgttg tctttaaaaa
    27421 aggcgaggcg gtgacggatt aattcaagcc agccaggttc ccaataatgc atatagctga
    27481 ggcttgatcc ggcagctaac gtcgcagcgg ctgcctgtaa aaaacgcctg cgtgtgatca
    27541 tcatccatcc ctgcaaaaaa tcgggcagcg tcatgctgcc cgtgtactga tttttagtcg
    27601 atggtacgca gcagttcgtt aatgccgact ttgccgcgag ttttcgcgtc aactttctta
    27661 acgataactg cacaataaag gctgtatttg ccatctttcg acggcaggtt gccagaaaca
    27721 accaccgaac cagccggaac gcgaccgtag tggatttcgc cggtttcacg gtcgtaaata
    27781 cgggtgctct gaccaatgta tacgcccata gagatgacgg aaccttcttc gacaatcacc
    27841 ccttcaacca cttcagagcg tgcaccgatg aagcaattat cttcaataat ggtcgggtta
    27901 gcctgcagcg gctccagcac gccgccgatg cctacaccgc cagacagatg gacgttttta
    27961 ccaatttgtg cacaggaacc gacagtggcc caggtatcaa ccatagtgcc ttcatcaaca
    28021 tatgcgccga tgttgacgta agacggcatc agcacggtgt tacgggcaat aaatgcaccc
    28081 tgacgtaccg ccgctggtgg cacaacgcgg aagccttctt tctgaaaacg cgcttcgtcg
    28141 tagtcggcga atttcatcgg cactttatcg aagtagcggc tttctgcccc ttcgatcacc
    28201 tgattatcat taatacggaa agagagcagc accgcttttt tcaaccactg atgcgtcacc
    28261 cactgaccgt caattttttc cgctacacgc agtgcgccgg aatccagcag ggcgatcacc
    28321 tgatttaccg cttcgcgggt tacggtgtct gcattggctg gcgtgatctc ggcacggcgt
    28381 tcaaaagcgg tttcaataat gttctgtaac tgctgcattg ttaaactctt ttcatatcag
    28441 taaacacatc accctttatc gtttggattg agggcctctg tcaaccgctg atgcacttcc
    28501 tgctgcaact cgttattaag cgcacgccgg tcagcggtgg caattatgaa taaatcttct
    28561 actcgctcgc caatggttgt aattcgggcg ccatgaagcg aaattcccag atcggcaaaa
    28621 attttcccga ctcgcgccag cagtccaggt tggtcgaggg cgatcagttc gaggaacgat
    28681 ttgcggtcgg tatgggtcgg caaaaacgtc acttcggttt caacagtaaa atggcgcaat
    28741 ttggcgggtt ggcgacgggg ctgcggtggc tgccagctac tttgcgtcag cacctgctcc
    28801 agaccaaacc gaataacctc atgacgatct gcggatagcg ggctaccatc gggttccagc
    28861 acgatgaagg tatccatcgc cataccgtcg cgagtggtga aaatttgtgc gtcgtgaaca
    28921 cttaaattgc ggcggtctaa ttcggcacag acggcggcaa acagataggg gcggtccggg
    28981 ctccagataa aaatctcggt gcctccacgc gtagcctgcg gactaagcaa taccagcggt
    29041 ttgcttaaat catgctgtaa taaatggcgc gcatgccagg ccagttgatt tgggctatgg
    29101 cggacaaaat agttagcacg acagcgtgac caaatttggt gcagcgcctc ttcgtcgatg
    29161 ttatccatgc gcagtagtgc cagcgcctgg agttggtgat ggcgaacccg ttcgcgcata
    29221 tccggcgtgt tttgcattcc gcgtcgtagc tgcttttcgg tggcgaaata gagttcgcgc
    29281 aacagacttt gcttccagct attccacagc gtttcgttgg tggcgcatat atcggccact
    29341 gtcaggcata ccagataacg aagtcggttt tctgtttgca cttcttcggc aaactgcttg
    29401 atcacttccg ggtcctgaat atcgcggcgt tgggcggtca ccgacatcag caagtgctgg
    29461 cgaaccagcc acgccaccag ctgtgtttcg cgcgagttaa gcccgtggag ttcggcaaaa
    29521 tgcaccacgt cttgtgcacc gagaatggag tggtcgccgc cacgcccttt ggcgatatcg
    29581 tgaaacagcg cggcgatgaa aatcagctca gttgacggca ggcgcggcca gacgtctaca
    29641 cacaacggat ggcgctggcg cgtttcttca ctggcaaaac tctccagttt cagcatcacg
    29701 cgaatagtat gttcatccac cgtgtaggcg tggaacaggt caaactgcat ctgcccgacg
    29761 atatgcgacc attggggcat atacgcgccg agcacgctat gacgatgcat cggtaatagt
    29821 ccgcgacgca ccgcaccagg gtgacgcaga atgctcaaaa acagttttcg cgcttccgga
    29881 atattacaca gcggttgttg caaatggcga cgggcgtggc gtaactggcg cagcgtggta
    29941 gagtaaatgc cggtgatcgc actgttgcgc accatggtgt agaacatacg caagatggct
    30001 tccggctggc gcataaatag tgtttcatca cgcaggtcga taagcgttcc gcgtagctga
    30061 aactcatcgt cgattggacg tggtttttcg tcggcgggaa gggcgaggat ggcttcatcg
    30121 aacagttgca gcagcatctg gttgagttca ctgacgcggc gtgtaacgcg gaagtaatcc
    30181 ttcatcatcc gctcgaccgg ttcgttacct tcgccactgt aattcagacg ctgggcgacg
    30241 ctaagctggc gatcaaacag caggcgatta tcgtaacggc tgacgaccag atgcagggca
    30301 aagcgaatac gccacaatat atgcagacat tcgtttaatt ccgctcgctc cgctgaggtt
    30361 aagaagccaa acccgaccat ttcatccagc gatgttgcgc caaaatgacg gcgggccacc
    30421 cattgcagag tgtgaatatc gcgcaagccg ccaggactac ttttgatgtc tggttcaagg
    30481 ttgtagctgg tgccgtggta acgctgatgg cgctggttct gttcttcaac tttcgctgcg
    30541 tagaacttgt cggaaggcca gaatccttcg ctaaaaatat gtttttgcag ttcgaggaag
    30601 agcgctacat cgccaattaa taagcgggat tcgattaaat tggtggcgac ggttaaatcc
    30661 gataaccctt ccagcatgca ctcttcaagc gtgcgcacgc tatgaccgac ttccagcttt
    30721 acatcccaga gcagcgttaa cagctcgccc actttttgcg cctgatcatc cgggagcttt
    30781 ttacggctta aaatcagtaa atcgatgtcg gaaagtggat gcagctcgcc acgaccataa
    30841 ccaccgacgg caaccaatgc caggtcggca atctggctga atcccgcttc aatccataat
    30901 cgttgcagga gttggtcgat aaactcggtg cgtgcctcaa tcaactgttc tgcggagatc
    30961 ccattgtcaa aggcatcacc cagccaacgc tggaaagtat cgatatgggc ttttatccca
    31021 cagacggtta attcatcacg gggccaggcg catggatttt gcggttgacc gggcagggtg
    31081 gggagagcgg tgtttgcgta ctgttctgga agggtattca ttgtgcgcca cccataagat
    31141 taaattatcg cattaaaaaa gccggcattc gccggcttca tcttattcgt cgtgcgagat
    31201 tatcgccggg atggtgtcat ccttgcgtag cgtcagaatt tcgcagccgt tatcagtcac
    31261 cacaatagta tgctcatatt gtgcagacaa gctgcgatct ttggttttta ccgtccagcc
    31321 atctttcatg gtgcggatct cttttttacc cgcgttgacc attggctcga tggtgaacgt
    31381 catcccaggt ttcagtacga cgttggtttc acgggagtca tagtgcagca cctgcggttc
    31441 ttcgtggaag ccgcgaccaa taccgtgtcc acaatattca cgaacgacgg agaagccttc
    31501 tgcttcgaca aatttctgaa ttgccgcacc gatttcgcgc agattaatgc caggttttac
    31561 catgcgtagc gccaggtaca ggctttcttg cgtgatgcgg cacagacgtt cgcccatgat
    31621 ggtcggctta ccgacgataa acattttcga ggtatcgccg tggaaaccat ctttgattac
    31681 ggtgacatca atgttaacga tatcgccatc tttcagcagc ttggcgtcgt ccgggatacc
    31741 gtggcacacc acttcattaa tagaaatgca aacggatttc ggatagccgt gatagccgag
    31801 gcaggcagaa accgcgtgtt gttcattaac aatgtaatca ttacagatgc gatccagctc
    31861 gccggtgctg acgcccggtt taacatacgg ttcgatcatc tccagcactt cggcagccag
    31921 tcggccagcg acgcgcattt tttcgatatc ttctggggtc ttgattgaga tagccattaa
    31981 ttctgtccat cagcgtcggt gataccgaca atatatatgt aagtgccgtc aatggtatca
    32041 cacccgggca aattgagaat cattctgaat ttcgccaaac gtgccactga aggttttcta
    32101 taatagaaaa ttcaacgtct gatgctgtac acagcgccaa caattattgg tgtccacgac
    32161 gtatttgtgg tataaagcgc gccggacttc cgatccattt cgtatacaca gactggacgg
    32221 aagcgacaat ctcactttgt gtaacaacac acacgtatcg gcacatattc cggggtgccc
    32281 tttggggtcg gtaatatggg atacgtggag gcataacccc aacttttata tagaggtttt
    32341 aatcatggca actgtttcca tgcgcgacat gctcaaggct ggtgttcact tcggtcacca
    32401 gacccgttac tggaacccga aaatgaagcc gttcatcttc ggtgcgcgta acaaagttca
    32461 catcatcaac cttgagaaaa ctgtaccgat gttcaacgaa gctctggctg aactgaacaa
    32521 gattgcttct cgcaaaggta aaatcctttt cgttggtact aaacgcgctg caagcgaagc
    32581 ggtgaaagac gctgctctga gctgcgacca gttcttcgtg aaccatcgct ggctgggcgg
    32641 tatgctgact aactggaaaa ccgttcgtca gtccatcaaa cgtctgaaag acctggaaac
    32701 tcagtctcag gacggtactt tcgacaagct gaccaagaaa gaagcgctga tgcgcactcg
    32761 tgagctggag aaactggaaa acagcctggg cggtatcaaa gacatgggcg gtctgccgga
    32821 cgctctgttt gtaatcgatg ctgaccacga acacattgct atcaaagaag caaacaacct
    32881 gggtattccg gtatttgcta tcgttgatac caactctgat ccggacggtg ttgacttcgt
    32941 tatcccgggt aacgacgacg caatccgtgc tgtgaccctg tacctgggcg ctgttgctgc
    33001 aaccgtacgt gaaggccgtt ctcaggatct ggcttcccag gcggaagaaa gcttcgtaga
    33061 agctgagtaa taaggcttga taactccccc aaaatagttc gagttgcaga aaggcggcaa
    33121 gctcgagaat tcccgggagc ttacatcagt aagtgaccgg gatgagcgag cgaagataac
    33181 gcatctgcgg cgcgaaatat gaagggggag agcccttata gaccaggtag tacacgtttg
    33241 gttagggggc ctgcatatgg cccccttttt cacttttata tctgtgcggt ttaatgccgg
    33301 gcagatcaca tctccgagga ttttagaatg gctgaaatta ccgcatccct ggtaaaagag
    33361 ctgcgtgagc gtactggcgc aggcatgatg gattgcaaaa aagcactgac tgaagctaac
    33421 ggcgacatcg agctggcaat cgaaaacatg cgtaagtccg gtgctattaa agcagcgaaa
    33481 aaagcaggca acgttgctgc tgacggcgtg atcaaaacca aaatcgacgg caactacggc
    33541 atcattctgg aagttaactg ccagactgac ttcgttgcaa aagacgctgg tttccaggcg
    33601 ttcgcagaca aagttctgga cgcagctgtt gctggcaaaa tcactgacgt tgaagtactg
    33661 aaagcacagt tcgaagaaga acgtgttgcg ctggtagcga aaattggtga aaacatcaac
    33721 attcgccgcg ttgctgcgct ggaaggcgac gttctgggtt cttatcagca cggtgcgcgt
    33781 atcggtgttc tggttgctgc taaaggcgct gacgaagagc tggttaaaca catcgctatg
    33841 cacgttgctg caagcaagcc agaattcatc aaaccagaag acgtatccgc tgaagtggta
    33901 gaaaaagaat accaggtaca gctggatatc gcgatgcagt ctggtaagcc gaaagaaatc
    33961 gcagagaaaa tggttgaagg ccgcatgaag aaattcaccg gcgaagtttc tctgaccggt
    34021 cagccgttcg ttatggaacc aagcaaaact gttggtcagc tgctgaaaga gcataacgct
    34081 gaagtgactg gcttcatccg cttcgaagtg ggtgaaggca tcgagaaagt tgagactgac
    34141 tttgcagcag aagttgctgc gatgtccaag cagtcttaat tatcgaaaag gagccgcctg
    34201 agggcggctt ctttttgtgc ccatcttgta aattcagcta acccttgtgg ggctgcgctg
    34261 aaaagcgacg tacaatgtcg ctagtattaa ttcatttcaa tcgttgacag tctcaggaaa
    34321 gaaacatggc taccaatgca aaacccgtct ataaacgcat tctgcttaag ttgagtggcg
    34381 aagctctgca gggcactgaa ggcttcggta ttgatgcaag catactggat cgcatggctc
    34441 aggaaatcaa agaactggtt gaactgggta ttcaggttgg tgtggtgatt ggtgggggta
    34501 acctgttccg tggcgctggt ctggcgaaag cgggtatgaa ccgcgttgtg ggcgaccaca
    34561 tggggatgct ggcgaccgta atgaacggcc tggcaatgcg tgatgcactg caccgcgcct
    34621 atgtgaacgc tcgtctgatg tccgctattc cattgaatgg cgtgtgcgac agctacagct
    34681 gggcagaagc tatcagcctg ttgcgcaaca accgtgtggt gatcctctcc gccggtacag
    34741 gtaacccgtt ctttaccacc gactcagcag cttgcctgcg tggtatcgaa attgaagccg
    34801 atgtggtgct gaaagcaacc aaagttgacg gcgtgtttac tgctgatccg gcgaaagatc
    34861 caaccgcaac catgtacgag caactgactt acagcgaagt gctggaaaaa gagctgaaag
    34921 tcatggacct ggcggccttc acgcttgctc gtgaccataa attaccgatt cgtgttttca
    34981 acatgaacaa accgggtgcg ctgcgccgtg tggtaatggg tgaaaaagaa gggactttaa
    35041 tcacggaata attcccgtga tggataaata agggtaagat tccgcgtaag tatcgcgggg
    35101 gcgtaagtct ggttataagg cgttattgtt gcaggcagtt tggtcacggc cagcgcgcag
    35161 caaccggagc gtacaaaagt acgtgaggat ggcgagcact gcccggggcc aaaatggcaa
    35221 ataaaatagc ctaataatcc agacgattac ccgtaatatg tttaatcagg gctatactta
    35281 gcacacttcc actgtgtgtg actgtctggt ctgactgaga caagttttca aggattcgta
    35341 acgtgattag cgatatcaga aaagatgctg aagtacgcat ggataaatgc gtagaagcgt
    35401 tcaaaaccca aatcagcaaa atacgcacgg gtcgtgcttc tcccagcctg ctggatggca
    35461 ttgtcgtgga atattacggc acgccgacgc cgctgcgtca gctggcaagc gtaacggtag
    35521 aagattcccg tactctgaaa atcaacgtgt ttgatcgttc aatgtctccg gccgttgaaa
    35581 aagcgattat ggcgtccgat cttggcctga acccgaactc tgcgggtagc gacatccgtg
    35641 ttccgctgcc gccgctgacg gaagaacgtc gtaaagatct gaccaaaatc gttcgtggtg
    35701 aagcagaaca agcgcgtgtt gcagtacgta atgtgcgtcg tgacgcgaac gacaaagtga
    35761 aagcactgtt gaaagataaa gagatcagcg aagacgacga tcgccgttct caggacgatg
    35821 tacagaaact gactgatgct gcaatcaaga aaattgaagc ggcgctggca gacaaagaag
    35881 cagaactgat gcagttctga tttcttgaac gacaaaaacg ccgctcagta gatccttgcg
    35941 gatcggctgg cggcgttttg ctttttattc tgtctcaact ctggatgttt catgaagcaa
    36001 ctcaccattc tgggctcgac cggctcgatt ggttgcagca cgctggacgt ggtgcgccat
    36061 aatcccgaac acttccgcgt agttgcgctg gtggcaggca aaaatgtcac tcgcatggta
    36121 gaacagtgcc tggaattctc tccccgctat gctgtaatgg atgatgaagc gagtgcgaaa
    36181 cttcttaaaa cgatgctaca gcaacagggt agccgcaccg aagtcttaag tgggcaacaa
    36241 gccgcttgcg atatggcagc gcttgaggat gttgatcagg tgatggcagc cattgttggc
    36301 gctgctgggc tgttacctac gcttgctgcg atccgcgcgg gtaaaaccat tttgctggcc
    36361 aataaagaat cactggttac ctgcggacgt ctgtttatgg acgccgtaaa gcagagcaaa
    36421 gcgcaattgt taccggtcga tagcgaacat aacgccattt ttcagagttt accgcaacct
    36481 atccagcata atctgggata cgctgacctt gagcaaaatg gcgtggtgtc cattttactt
    36541 accgggtctg gtggcccttt ccgtgagacg ccattgcgcg atttggcaac aatgacgccc
    36601 gatcaagcct gccgtcatcc gaactggtcg atgggacgta aaatttctgt cgattcggct
    36661 accatgatga acaaaggtct ggaatacatt gaagcgcgtt ggctgtttaa cgccagcgcc
    36721 agccagatgg aagtgctgat tcacccgcag tcagtgattc actcaatggt gcgctatcag
    36781 gacggcagtg ttctggcgca gctgggggaa ccggatatgc gtacgccaat tgcccacacc
    36841 atggcatggc cgaatcgcgt gaactctggc gtgaagccgc tcgatttttg caaactaagt
    36901 gcgttgacat ttgccgcacc ggattatgat cgttatccat gcctgaaact ggcgatggag
    36961 gcgttcgaac aaggccaggc agcgacgaca gcattgaatg ccgcaaacga aatcaccgta
    37021 gctgcttttc ttgcgcaaca aatccgcttt acggatatcg ccgcgttgaa tttatccgta
    37081 ctggaaaaaa tggatatgcg cgaaccacaa tgtgtggacg atgtgttatc tgttgatgcg
    37141 aacgcgcgtg aagtcgccag aaaagaggtg atgcgtctcg caagctgagg ataatccggc
    37201 tacagagagt cgcgctattt gttagcgtag ggcttcagtg atatagtctg cgccatctga
    37261 tcgtaagtag ttggctttat aaggtcaggt atgccgtggt tttacacggc ttttttttgt
    37321 ataggcttca gtattcctga gtaccgtaaa ccctgtcagg gaataagaaa cgcgtgatgt
    37381 tgtctgctac tcaaccactt agcgaaaaat tgccagcgca tggctgtcgt catgttgcga
    37441 tcattatgga cggcaatggt cgctgggcaa aaaagcaagg gaagattcgt gcctttgggc
    37501 ataaagccgg ggcaaaatcc gtccgccggg ctgtctcttt tgcggccaac aacggtattg
    37561 aggcgttaac gctgtatgcc tttagtagtg aaaactggaa ccgaccagcg caggaagtca
    37621 gtgcgttaat ggaactgttt gtgtgggcgc tagatagcga agtaaaaagt ctgcaccgac
    37681 ataacgtgcg tctgcgtatt attggcgata ccagtcgctt taactcgcgt ttgcaagaac
    37741 gtattcgtaa atctgaagcg ctaacagccg ggaataccgg tctgacgctg aatattgcgg
    37801 cgaactacgg tggacgttgg gatatagtcc agggagtcag gcaactggct gaaaaggtgc
    37861 agcaaggaaa cctgcaacca gatcagatag atgaagagat gctaaaccag catgtctgta
    37921 tgcatgaact ggcccctgta gatttagtaa ttaggactgg gggggagcat cgcattagta
    37981 actttttgct ttggcaaatt gcctatgccg aactttactt tacagatgtt ctctggcccg
    38041 atttcgatga acaagacttt gaaggggcgt taaatgcctt tgctaatcga gagcgtcgtt
    38101 tcggcggcac cgagcccggt gatgaaacag cctgatgggg gtcgcttttg ctgaagtatc
    38161 gcctgatatc tgcttttgtg ttaatacccg ttgtcatcgc ggcgttgttt ctgttgccgc
    38221 cggtggggtt cgccattgta acgctggtgg tctgcatgct ggcagcgtgg gaatggggac
    38281 agcttagcgg ttttaccact cgttcgcagc gagtatggtt ggcggtgtta tgcgggttat
    38341 tgttggcgct gatgcttttt ctgttgccgg aatatcaccg aaatattcat caaccgctgg
    38401 ttgaaatctc actttgggct tcgctgggtt ggtggattgt cgcgctattg ctggtgctgt
    38461 tttacccagg ttccgcagca atctggcgta actctaaaac attgcgcctt atttttggcg
    38521 tgctaaccat tgttcccttc ttctggggca tgctggcgtt acgggcctgg cactacgacg
    38581 agaatcatta cagtggcgca atatggctgc tctatgtcat gatcctggta tggggcgctg
    38641 actctggcgc atatatgttt ggcaaattgt ttggtaaaca taagctggca ccgaaggttt
    38701 ctccgggtaa aacctggcaa ggctttatcg gtggactagt gaccgcagcg gtaatctcat
    38761 ggggttatgg catgtgggtg aatctcgacg tcgctcccgt caccttactc atttgctcta
    38821 ttgtcgcagc gttagcctca gtgctcggcg atctgaccga aagtatgttt aagcgtgaag
    38881 caggaattaa ggacagcggt catttaattc caggacacgg tggtatttta gatcgtattg
    38941 atagcctgac ggctgcggta ccggtctttg cttgcttgtt gttactggta ttcaggacgc
    39001 tttaacggaa ggtaatatgc tgagttttct ctgggatttg gcttcgttca tcgttgcact
    39061 gggtgtactt atcaccgtgc atgaatttgg tcatttctgg gttgcccggc gttgtggtgt
    39121 tcgcgttgag cgtttctcaa tagggtttgg taaggcgctc tggcggcgaa ctgataagct
    39181 cggcaccgaa tatgttatcg ccctgatccc gttgggcggt tatgtcaaaa tgctggatga
    39241 gcgcgcagaa ccggtcgttc cggaactccg ccaccatgcc ttcaataata aatctgtcgg
    39301 ccaacgagcg gcgattattg ccgcaggtcc ggttgcaaac ttcatttttg ctatctttgc
    39361 ctactggctg gtttttatta ttggtgtgcc tggcgtacgt ccggtggttg gtgaaatagc
    39421 agccaattcg atagctgcgg aagcacaaat tgcaccaggt acggaactaa aagccgtaga
    39481 tggtatcgaa acgcctgatt gggatgccgt gcgtttgcag ttggtcgata aaattggcga
    39541 tgaaagcacc accattacgg tagcgccatt tggcagcgac caacggcggg atgtaaagct
    39601 cgatttacgt cactgggcgt ttgagcctga taaagaagat ccggtatctt cgttggggat
    39661 tcgtcctcgt gggccgcaaa ttgaacctgt actggaaaat gtgcagccaa actcggcggc
    39721 aagcaaggca ggtttgcaag caggcgacag gatcgttaaa gtcgatggtc agcccttaac
    39781 gcagtgggtg acctttgtga tgcttgtccg ggataacccg ggtaaatcct tagcgttaga
    39841 aatcgaaagg caggggagtc ccttgtcttt gacattaatc ccggagagta aaccgggtaa
    39901 gggtaaagcg attggttttg tcggtattga gccgaaagtc attcctttgc cagatgagta
    39961 taaagttgta cgccagtatg ggccgttcaa cgctatcgtc gaagccacgg acaaaacgtg
    40021 gcagttgatg aagctgacgg tcagtatgct gggaaaattg atcaccggtg atgtgaaact
    40081 gaacaacctc agtgggccga tctctatcgc caagggggct gggatgacag cggaactcgg
    40141 ggtagtttat tacctgccgt ttcttgcgct tattagcgtg aacttaggga taattaacct
    40201 gtttccgttg cccgtacttg acggggggca tctgctgttc cttgcgatcg aaaagatcaa
    40261 gggcggaccg gtatccgagc gggttcaaga cttttgttat cgcattggct cgattctgct
    40321 ggtgctgtta atggggcttg cacttttcaa tgatttctct cggttatgag agttagttag
    40381 gaagaacgca taataacgat ggcgatgaaa aagttgctca tagcgtcgct gctgtttagc
    40441 agcgccaccg tatacggtgc tgaagggttc gtagtgaaag atattcattt cgaaggcctt
    40501 cagcgtgtcg ccgttggtgc ggccctcctc agtatgccgg tgcgcacagg cgacacggtt
    40561 aatgatgaag atatcagtaa taccattcgc gctctgtttg ctaccggcaa ctttgaggat
    40621 gttcgcgtcc ttcgtgatgg tgataccctt ctggttcagg taaaagaacg tccgaccatt
    40681 gccagcatta ctttctccgg taacaaatcg gtgaaagatg acatgctgaa gcaaaacctc
    40741 gaggcttctg gtgtgcgtgt gggcgaatcc ctcgatcgca ccaccattgc cgatatcgag
    40801 aaaggtttgg aagacttcta ctacagcgtc ggtaaatata gcgccagcgt taaagctgtc
    40861 gtgaccccgc tgccgcgcaa ccgtgttgac ctaaaactgg tgttccagga aggtgtgtca
    40921 gctgaaatcc agcaaattaa cattgttggt aaccatgctt tcaccaccga tgaactgatc
    40981 tctcatttcc aactgcgtga cgaagtgccg tggtggaacg tggtaggcga tcgtaaatac
    41041 cagaaacaga aactggcggg cgaccttgaa accctgcgca gttactatct ggatcgcggt
    41101 tatgcccgtt tcaacatcga ttctacccag gtcagtctga cgccagataa aaaaggtatt
    41161 tacgtcacgg tgaacatcac cgaaggcgat cagtacaagc tttctggcgt tgaagttagc
    41221 ggcaatcttg ccgggcactc cgctgaaatt gagcaactga ctaagatcga gccgggcgag
    41281 ctgtataacg gcaccaaagt gaccaaaatg gaagatgaca ttaaaaagct tcttggtcgc
    41341 tatggttatg cctatccgcg cgtacagtcg atgcctgaaa ttaacgatgc cgacaaaacc
    41401 gttaaattac gcgtgaacgt tgatgcgggt aaccgtttct atgtgcgtaa gatccgtttt
    41461 gaaggtaacg atacctcgaa agatgccgtc ctgcgtcgcg aaatgcgtca gatggaaggt
    41521 gcatggctgg ggagcgatct ggtcgatcag ggtaaggagc gtctgaaccg tctgggcttc
    41581 tttgaaactg tcgataccga tacccaacgt gttccgggga gcccggatca ggttgatgta
    41641 gtttacaagg tgaaagaacg taacaccggt agcttcaact tcgggattgg ttacggtact
    41701 gaaagtggcg tgagcttcca ggctggtgtg caacaggata actggttagg tacgggttat
    41761 gctgttggta tcaacgggac caaaaacgat taccagacct atgctgaact gtcggtaacc
    41821 aacccgtact tcaccgtaga tggcgtaagc ctcggtggtc gtctcttcta taatgacttc
    41881 caggcagatg acgccgacct gtccgactat accaacaaga gttatggtac agacgtgacg
    41941 ttgggcttcc cgatcaacga atataactcg ctgcgtgcag gtctgggtta tgtacataac
    42001 tccctgtcca acatgcagcc gcaggttgcg atgtggcgtt atctgtactc tatgggtgaa
    42061 catccgagca cctctgatca ggataacagc ttcaaaacgg acgacttcac gttcaactat
    42121 ggttggacct ataacaagct tgaccgtggt tacttcccga cagatggttc acgtgtcaac
    42181 ctgaccggta aagtgaccat tccgggatcg gataacgaat actacaaagt gacgttagac
    42241 acggcgactt atgtgccgat tgatgatgat cacaaatggg ttgttctggg tcgtacccgc
    42301 tggggttatg gtgatggttt aggcggcaaa gagatgccgt tctatgagaa cttctatgcc
    42361 ggtggttcca gcaccgtgcg tggcttccag tccaatacca ttggtccgaa agcagtttac
    42421 ttcccgcatc aggccagtaa ttatgatccg gactatgatt acgaatgtgc gactcaggac
    42481 ggcgcgaaag atctgtgtaa atcggatgat gctgtaggcg gtaacgccat ggcggttgcc
    42541 agcctcgagt tcatcacccc gacgccgttt attagcgata agtatgctaa ctcggttcgt
    42601 acttccttct tctgggatat gggtaccgtt tgggatacaa actgggattc cagccaatat
    42661 tctggatatc cggactatag tgatccaagc aatatccgta tgtctgcggg tatcgcatta
    42721 caatggatgt ccccattggg gccgttggtg ttctcctacg cccagccgtt caaaaagtac
    42781 gatggagaca aggcagaaca gttccagttt aacatcggta aaacctggta agtgttctcc
    42841 acaaaggaat gtagtggtag tgtagcgatg actttaggcg atcaatataa gatcgccggg
    42901 ccacgcaaag aactgcaccc tccggtgcaa atgggatggt aaggagttta ttgtgaaaaa
    42961 gtggttatta gctgcaggtc tcggtttagc actggcaact tctgctcagg cggctgacaa
    43021 aattgcaatc gtcaatatgg gcagcctgtt ccagcaggta gcgcagaaaa ccggtgtttc
    43081 taacacgctg gaaaacgagt tcaaaggccg tgccagcgaa ctgcagcgta tggaaaccga
    43141 tctgcaggct aaaatgaaaa agctgcagtc catgaaagcg ggcagcgatc gcactaagct
    43201 ggaaaaagac gtgatggctc agcgccagac ttttgctcag aaagcgcagg cttttgagca
    43261 ggatcgcgca cgtcgttcca acgaagaacg cggcaaactg gttactcgta tccagactgc
    43321 tgtgaaatcc gttgccaaca gccaggatat cgatctggtt gttgatgcaa atgccgttgc
    43381 ttacaacagc agcgatgtaa aagacatcac tgccgacgta ctgaaacagg ttaaataagt
    43441 aatgccttca attcgactgg ctgatttagc gcagcagttg gatgcagaac tacacggtga
    43501 tggcgatatc gtcatcaccg gcgttgcgtc catgcaatct gcacaaacag gtcacattac
    43561 gttcatggtt aaccccaaat accgtgagca tttaggcttg tgccaggcgt ccgcggttgt
    43621 catgacccag gacgatcttc ctttcgcgaa aagtgccgcg ctggtagtga agaatcccta
    43681 cctgacttac gcgcgtatgg cgcaaatttt agataccacg ccgcagcccg cgcagaacat
    43741 tgcacccagt gcggtgattg acgcgacggc gaagctgggt aacaacgtat cgattggcgc
    43801 taacgcggtg attgagtccg gcgttgaact gggcgataac gtgattatcg gtgccggttg
    43861 cttcgtaggt aaaaacagca aaatcggtgc aggttcgcgt ctctgggcga acgtaaccat
    43921 ttaccatgag atccagatcg gtcagaattg cctgatccag tccggaacag tggtaggcgc
    43981 agacggcttt ggttatgcca acgatcgtgg taactgggtg aagatcccac agattggtcg
    44041 cgtaattatt ggcgatcgcg tggagatcgg tgcctgcact accattgatc gtggcgcgct
    44101 ggatgacact gttattggca atggtgtgat cattgataac cagtgccaga ttgcacataa
    44161 cgtcgtgatt ggcgacaata cggcagttgc cggtggcgtc attatggcgg gcagcctgaa
    44221 aattggtcgt tactgcatga tcggcggagc cagcgtaatc aacgggcata tggaaatatg
    44281 cgacaaagtg acggttacgg gcatgggtat ggtgatgcgt cccatcactg aaccaggcgt
    44341 ctattcctca ggcattccgc tgcaacccaa caaagtctgg cgcaaaaccg ctgcactggt
    44401 gatgaacatt gatgacatga gcaagcgtct gaaatcgctt gagcgcaagg ttaatcaaca
    44461 agactaacgt tccatctttt gttcgccaaa ctttacggcc tgtctcattc ttacgattgc
    44521 ggcaggccgt gttattattg tcgtttctta tattttgaca ggaagagtat cttgactact
    44581 aacactcata ctctgcagat tgaagagatt ttagaacttc tgccgcaccg tttcccgttc
    44641 ttactggtgg atcgcgtgct ggattttgaa gaaggtcgtt ttctgcgcgc agtaaaaaat
    44701 gtctctgtca atgagccatt cttccagggc catttccctg gaaaaccgat tttcccgggt
    44761 gtgctgattc tggaagcaat ggcacaggca acaggtattc tggcgtttaa aagcgtagga
    44821 aaactggaac cgggtgagct gtactacttc gccggtattg acgaagcgcg ctttaagcgc
    44881 ccggtcgtgc ctggcgatca aatgatcatg gaagtcactt tcgaaaaaac gcgccgcggc
    44941 ctgacccgtt ttaaaggggt tgctctggtc gacggtaaag tagtttgcga agcaacgatg
    45001 atgtgtgctc gtagccggga ggcctgatac gtgattgata aatccgcctt tgtgcatcca
    45061 accgccattg tggaagaggg cgcgtcgatt ggcgcgaacg ctcacattgg tcctttttgt
    45121 atcgttggac cccatgtcga aattggtgag ggtaccgtac tgaaatctca cgttgtcgtg
    45181 aatggtcata ctaaaattgg ccgcgataat gagatttatc agttcgcctc catcggcgaa
    45241 gttaaccagg atctgaaata tgctggcgaa ccgacccgcg tggaaatcgg cgatcgtaac
    45301 cgcattcgcg aaagcgtcac cattcatcgt ggcacagtcc agggcggtgg attgacgaag
    45361 gtgggcagcg acaacttact gatgatcaat gcgcacattg cgcacgattg tacggtaggt
    45421 aaccgctgca tcctcgccaa caacgcaacg ctggcgggtc acgtatcggt tgacgacttc
    45481 gcgatcatcg gcggcatgac cgcagtccat cagttctgca tcattggtgc gcatgtgatg
    45541 gttggcggct gctccggtgt ggcgcaggac gtccctcctt atgtcattgc gcagggtaac
    45601 cacgcaacgc cgttcggtgt caatatcgaa gggctgaagc gccgcggatt cagccgtgag
    45661 gcgattaccg ctatccgcaa tgcgtataag ctgatttatc gtagcggtaa aacgctcgat
    45721 gaagtgaaac cggaaattgc tgaactggcg gaaacatatc cggaagtgaa agcctttacc
    45781 gatttctttg cacgctcaac gcgcggtctg attcgttaat gactgaacag cgtccattaa
    45841 cgattgccct ggtcgccgga gaaacctccg gcgatatcct gggggccggt ttaatccgcg
    45901 ctctgaaaga acgtgtgccc aacgcccgct ttgttggtgt tgccgggcca cgaatgcagg
    45961 ctgaaggctg cgaagcctgg tacgaaatgg aagaactggc agtgatgggc attgttgaag
    46021 tgctcggtcg tctgcgtcgc ttactacata ttcgtgccga tctgacaaag cgttttggcg
    46081 aactgaagcc agatgttttt gttggtattg atgcgcctga cttcaatatt actcttgaag
    46141 gtaacctcaa aaagcagggt atcaaaacca ttcattacgt cagtccgtcc gtctgggcgt
    46201 ggcgacagaa acgcgttttc aaaataggca gagccaccga tctggtgctc gcatttctgc
    46261 ctttcgaaaa ggcgttttat gacaaataca acgtaccgtg ccgctttatc ggtcatacca
    46321 tggctgatgc catgccatta gatccagata aaaatggtgc ccgtgatgtg ctggggatcc
    46381 cttacgatgc ccactgtctg gcattgttgc cgggcagccg tggtgcagaa gtcgaaatgc
    46441 ttagtgccga tttcctgaaa acggcccagc ttttgcgcca gacatatccg gatctcgaaa
    46501 tcgtggtgcc gctggtgaat gccaaacgcc gcgagcagtt tgaacgcatc aaagctgcag
    46561 tcgcgccaga cctgtcggtt catttgctgg atggaatggg ccgtgaggcg atggtcgcca
    46621 gtgatgcggc gctactggcg tcgggtacgg cagcactgga gtgtatgctg gctaaatgcc
    46681 cgatggtagt gggatatcgc atgaagcctt tcaccttctg gctggcgaag cgactggtga
    46741 aaactgatta tgtctcgctg ccaaatctgc tggcgggcag agagttagtc aaagagctat
    46801 tgcaggaaga gtgtgagccg caaaaattgg ctgcggcgct gttaccgctg ttggcgaacg
    46861 ggaaaaccag ccatgcgatg cacgacacct tccgcgagct gcatcagcag atccgctgca
    46921 atgccgatga gcaggcggca caagccgttc tggagttagc acaatgatcg aatttgtcta
    46981 tccgcacacg cagctggttg cgggtgtgga tgaagtcgga cgcgggccgt tagttggcgc
    47041 ggtcgtcacc gctgcggtga tccttgaccc ggcgcgccca attgccgggc tgaatgattc
    47101 caaaaagctg agcgaaaaac gccgtctggc gctctgtgaa gagatcaaag agaaagcgtt
    47161 gagctggagt ctgggccgcg cggaacctca cgaaatcgac gaactgaata ttttacatgc
    47221 gaccatgctg gcgatgcagc gtgccgtcgc tggactgcat attgcgccgg aatatgtgtt
    47281 gattgatggt aaccgctgcc cgaaattacc gatgccttcg atggctgtgg tgaaaggcga
    47341 tagccgcgta ccggaaatca gtgccgcgtc tatcctggcg aaagtgacgc gtgacgccga
    47401 aatggcagcg ctggatattg ttttcccgca atatggtttt gcccaacaca aagggtatcc
    47461 aaccgctttt catctggaaa aactggctga acacggcgcg accgaacacc atcggcgcag
    47521 ctttgggcct gtcaaacgcg cactgggact tgcgtcctga ttcttgtgtc gagattaagt
    47581 aaaccggaat ctgaagatgt ctgaaccacg tttcgtacac ctgcgggtgc acagcgacta
    47641 ctcgatgatc gatggcctgg ccaaaaccgc accgctggta aaaaaggcgg cagcgttggg
    47701 tatgccagca ctggcgatca ccgatttcac caacctttgc ggtctggtga agttctacgg
    47761 agcgggacat ggcgcaggga ttaagcccat cgtcggggcg gattttaacg tccagtgcga
    47821 cctgctgggt gatgagttaa ctcacctgac ggtgctggcg gcgaacaata ccggctatca
    47881 gaatctgacg ttgctgatct caaaagcgta tcagcgcggg tacggtaccg ccgggccgat
    47941 catcgatcgc gactggctta tcgaattaaa tgaagggttg atccttcttt ccggcgggcg
    48001 catgggcgac gttggacgca gtcttttgcg tggtaacagc gcgctggtag atgagtgtgt
    48061 cgcgttttat gaagaacact tcccggatcg ctattttctc gagctgatcc gcaccggcag
    48121 gccggacgaa gaaagctatc tgcacgctgc ggtggaactg gcggaagcac gcggtttgcc
    48181 cgttgtggcg accaacgacg tgcgctttat cgacagcagc gactttgacg cacacgaaat
    48241 ccgcgtcgcg attcacgacg gctttaccct cgacgatcct aaacgcccgc gtaactattc
    48301 gccgcagcaa tatatgcgta gcgaagagga gatgtgtgag ctgtttgccg acatccccga
    48361 agcccttgcc aacaccgttg agatcgccaa acgctgtaac gtgaccgtgc gccttggtga
    48421 atacttcctg ccgcagttcc cgaccgggga catgagcacc gaagattatc tggtcaagcg
    48481 tgcaaaagag ggcctggaag agcgtctggc atttttattc cctgacgagg aagaacgtct
    48541 taagcgtcgt ccggagtatg acgaacgtct ggatactgaa cttcaggtta tcaaccagat
    48601 gggcttcccg ggttacttcc tcatcgttat ggaatttatc cagtggtcga aagataacgg
    48661 tgtaccggta gggccaggcc gtggttctgg tgcagggtca ctggtggcct acgcgttaaa
    48721 aatcaccgat ctcgatccgc tggaatttga cctgctgttc gaacgtttcc ttaacccgga
    48781 acgtgtctcc atgcctgact tcgacgttga cttctgtatg gagaaacgcg accaggttat
    48841 cgagcacgta gcggacatgt acggtcgcga tgcggtatcg cagattatca ccttcggtac
    48901 gatggcggcg aaagcggtga tccgcgacgt aggccgtgta ttggggcatc cgtacggatt
    48961 tgtcgatcgt atctcgaaac tgatcccgcc cgatccgggt atgacgctgg cgaaagcgtt
    49021 tgaagccgag ccacagctgc cggaaatcta cgaagcggat gaagaagtta aggcgctgat
    49081 cgacatggcg cgcaaactgg aaggggtcac ccgtaacgcc ggtaagcacg ccggtggggt
    49141 ggttatcgcg ccgaccaaaa ttactgattt tgcgccgctt tattgcgatg aagagggcaa
    49201 acatccggtc acccagtttg ataaaagcga cgttgaatac gccgggctgg tgaagttcga
    49261 cttcctcggt ttgcgtacgc tcaccatcat caactgggcg ctggagatga tcaacaagcg
    49321 gcgggcgaag aatggcgagc cgccgctgga tatcgccgcg atcccgctgg atgacaagaa
    49381 aagcttcgac atgctgcaac gctcggaaac cacggcggta ttccagcttg aatcgcgcgg
    49441 catgaaggac ctgatcaagc gtctgcaacc tgactgcttc gaagatatga tcgcactggt
    49501 ggcactgttc cgccccggcc cgttgcaatc agggatggtg gataacttta tcgaccgtaa
    49561 acatggtcgc gaagagatct cctatccgga cgtgcagtgg cagcatgaaa gcctgaaacc
    49621 ggtactggag ccaacctacg gcatcatcct gtatcaggaa caggtcatgc agattgcgca
    49681 ggtgctttct ggttataccc tcggtggcgc ggatatgctg cgtcgtgcga tgggtaagaa
    49741 aaagccggaa gagatggcta agcagcgttc tgtatttgct gaaggtgcag aaaagaacgg
    49801 aatcaacgct gaactggcga tgaaaatctt cgacctggtg gagaaattcg cgggttacgg
    49861 atttaacaaa tcgcactctg cggcctatgc tttggtgtca tatcaaacgt tatggctgaa
    49921 agcgcactat ccggcggagt ttatggcggc ggtaatgacc gccgatatgg acaataccga
    49981 gaaggtggtg ggcctggtgg atgagtgctg gcggatgggg ctgaaaatcc tgccgccgga
    50041 tataaactcc ggtctttacc atttccacgt caacgacgac ggcgaaatcg tgtatggtat
    50101 tggcgcgatc aaaggggtcg gtgaaggtcc gattgaggcg atcatcgaag cccgcaataa
    50161 aggcggctac ttccgcgaac tgtttgatct ctgcgcccgt accgacacca aaaagttaaa
    50221 ccggcgggtg ctggaaaaac tgatcatgtc cggggcgttt gaccgtcttg ggccacaccg
    50281 cgcggcgctg atgaactcgc tgggcgatgc gttaaaagcg gcagatcaac acgcgaaagc
    50341 ggaagctatc ggtcaggccg atatgttcgg cgtgctggcc gaagagccgg aacaaattga
    50401 acaatcctac gccagctgcc aaccgtggcc ggagcaggtg gtgttagatg gggaacgtga
    50461 aacgttaggt ctgtacctga cgggacaccc tatcaaccag tatttaaaag agattgagcg
    50521 ttatgtcgga ggcgtaaggc tgaaagacat gcacccgaca gaacgtggta aagtcatcac
    50581 ggctgcgggg ctcgttgttg ccgcgcgggt tatggtcacc aagcgcggca atcgtatcgg
    50641 tatctgcacg ctggatgacc gttccgggcg gctggaagtg atgttgttta ctgacgccct
    50701 ggataaatac cagcaattgc tggaaaaaga ccgcatactt atcgtcagcg gacaggtcag
    50761 ctttgatgac ttcagcggtg ggcttaaaat gaccgctcgc gaagtgatgg atattgacga
    50821 agcccgggaa aaatatgctc gcgggcttgc tatctcgctg acggacaggc aaattgatga
    50881 ccagctttta aaccgactcc gtcagtctct ggaaccccac cgctctggga caattccagt
    50941 acatctctac tatcagaggg cggatgcacg cgcgcgattg cgttttggcg cgacgtggcg
    51001 tgtctctccg agcgatcgtt tattaaacga tctccgtggc ctcattggtt cggagcaggt
    51061 ggaactggag tttgactaat acaggaatac tatgagtctg aatttccttg attttgaaca
    51121 gccgattgca gagctggaag cgaaaatcga ttctctgacc gcggttagcc gtcaggatga
    51181 gaaactggat attaacatcg atgaagaagt gcatcgtctg cgtgaaaaaa gcgtagaact
    51241 gacgcgtaaa atcttcgccg atctcggtgc atggcagatt gcgcaactgg cacgccatcc
    51301 acagcgtcct tataccctgg attacgttcg cctggcattt gatgaatttg acgaactggc
    51361 tggcgaccgc gcgtatgcag acgataaagc tatcgtcggt ggtatcgccc gtctcgatgg
    51421 tcgtccggtg atgatcattg gtcatcaaaa aggtcgtgaa accaaagaaa aaattcgccg
    51481 taactttggt atgccagcgc cagaaggtta ccgcaaagca ctgcgtctga tgcaaatggc
    51541 tgaacgtttt aagatgccta tcatcacctt tatcgacacc ccgggggctt atccgggcgt
    51601 gggcgcagaa gaacgtggtc agtctgaagc cattgcacgc aacctgcgtg aaatgtctcg
    51661 cctcggcgta ccggtagttt gtacggttat cggtgaaggt ggttctggtg gtgcgctggc
    51721 gattggcgtg ggcgataaag tgaatatgct gcaatacagc acctattccg ttatctcgcc
    51781 ggaaggttgt gcatccattc tgtggaagag cgctgacaaa gcgccgctgg cggctgaagc
    51841 gatgggtatc atcgctccgc gtctgaaaga gctgaaactg atcgactcca tcatcccgga
    51901 accgctgggc ggtgctcacc gtaacccgga agcgatggcg gcgtctctga aagcgcagct
    51961 gctggcggat ctggccgatc tcgacgtgtt aagcactgaa gatttaaaaa atcgtcgtta
    52021 tcagcgcctg atgagctacg gctacgcgta atcggcaaat gttctgaaaa gggtcacttc
    52081 ggtggccctt ttttatcgcc acggtttgag caggctatga ttaaggaagg attttccagg
    52141 aggaacacat gaacatcatt gccattatgg gaccgcatgg cgtcttttat aaagatgagc
    52201 ccatcaaaga actggagtcg gcgctggtgg cgcaaggctt tcagattatc tggccacaaa
    52261 acagcgttga tttgctgaag tttatcgaac ataacccacg aatttgcggc gtgatttttg
    52321 actgggatga gtacagtctc gatttatgta gcgatatcaa tcaacttaat gaatatctcc
    52381 cgctttatgc cttcatcaac acccactcga cgatggatgt cagcgtgcag gatatgcgga
    52441 tggcgctctg gttttttgaa tatgcgctgg ggcaggcgga agatatcgcc attcgtatgc
    52501 gtcagtacac caacgaatat cttgataaca ttacgccgcc gttcacgaaa gccttgttta
    52561 cctacgtcaa agagcggaag tacacctttt gtacgccggg gcatatgggc ggcaccgcat
    52621 atcaaaaaag cccggttggc tgtctgtttt atgatttttt cggcgggaat accctcaagg
    52681 cggacgtctc tatttcggtc accgagcttg gttcgttgct cgaccacacc gggccacacc
    52741 tggaagcgga agagtacatc gcgcggacgt ttggcgcgga acagagttat atcgttacca
    52801 acggaacatc gacgtcgaac aaaattgtgg gtatgtacgc cgcgccattc ggcagtacgc
    52861 tgttgatcga ccgcaattgt cataaatcgc tggcgcatct gttgatgatg aacgatgtag
    52921 tgccagtctg gctgaaaccg acgcgtaatg cgttggggat tctgggtggg atcccgcgcc
    52981 gtgaatttac tcgcgacagc atcgaagaga aagtcgctgc caccacgcag gcacaatggc
    53041 cggttcatgc ggtgatcacc aactccacct atgatggctt gctctacaac accgactgga
    53101 tcaaacagac gctggatgtc ccgtcgattc acttcgattc tgcctgggtg ccgtacaccc
    53161 attttcatcc aatctaccag ggtaaaagtg gtatgagcgg cgagcgtgtt gcaggaaaag
    53221 tgatcttcga aacgcagtcg acccacaaaa tgctggcggc gttatcgcag gcgtcgctga
    53281 tccacattaa aggtgagtat gacgaagagg cgtttaacga agcctttatg atgcatacca
    53341 ccacctcgcc cagttatccc attgttgctt ctgttgagac ggcggcggcg atgttgcgtg
    53401 gtaatccggg caaacggctg attaaccgtt cagtagaacg agctctgcat tttcgcaaag
    53461 aggtccagcg gctgcgggaa gagtctgacg gctggttttt cgatatctgg caaccgccgc
    53521 aggtggatga agccgaatgc tggcccgttg cgcctggcga acagtggcac ggctttagcg
    53581 atgcggatgc caatcacatg tttctcgatc cggttaaagt cactattttg acaccgggga
    53641 tggacgagca gggcaatatg agcgaggagg ggatcccggc ggcgctggtg gcaaaattcc
    53701 tcgacgaacg tgggatcgta gtagagaaaa ccggccctta taacctgctg tttctcttta
    53761 gtattggcat cgataaaacc aaagcaatgg gattattgcg tgggttgacg gaatttaagc
    53821 gctcttacga tctcaacctg cggatcaaaa atatgctgcc cgatctctat gcagaagatc
    53881 ccgatttcta ccgcaatatg cgtattcagg atctggcgca agggatccat aagctgattc
    53941 gtaaacacga tcttcccggt ttgatgttgc gggcatttga taccttgccg gagatgatca
    54001 tgacgccaca tcaggcatgg cagagacaga ttaaaggcga agtagaaacc attgcgctgg
    54061 aacaactggt cggtagagta tcggcaaata tgatcctgcc ttatccaccc ggcgtaccgc
    54121 tgctgatgcc gggagaaatg cttaccgaag agagccgcac ggtactcgat tttctactga
    54181 tgctttgttc cgtagggcaa cattaccccg gttttgaaac ggatattcac ggcgcgaaac
    54241 aggacgaaga cggtgtttac cgcgtacgag tcctaaaaat ggcaggataa cttgccagag
    54301 cggcttccgg gcgagtaacg tgctgttaac aaataaagga gacgttatgc tgggtttaaa
    54361 acaggttcac catattgcga ttattgcgac ggattatgcg atgagcaaag ctttctactg
    54421 cgatattctt ggtttcacgc tgcaaagcga agtctatcgc gaagcgcgcg actcgtggaa
    54481 aggggatttg gcgcttaatg ggcaatatgt gattgagctt ttctcatttc cgttcccgcc
    54541 ggagcgcccc agccgaccgg aagcttgcgg cctgcgtcat ctggctttta gcgttgatga
    54601 tatcgatgcg gcagtggcgc accttgaaag ccataacgtg aagtgtgaag ccatccgtgt
    54661 cgatccctac acgcaaaaac gcttcacgtt ctttaacgat ccggacgggc tgccgttgga
    54721 actgtatgag cagtaaggct tgtcatcgcc gcatttgccc ggtaacgtgc ccggcattgc
    54781 tactgtaaaa tcgcaccatc atgacactca cgctcaatag acaacttctc tcctcacgcc
    54841 agattctggt ggcctttagc ggcggccttg attctactgt cttactgcat caactggtgc
    54901 agtggcggac ggaaaatccg ggcgtcactc tgcgcgctat ccatgtgcat cacggtttaa
    54961 gcgccaatgc tgatgcctgg gtgaaacatt gtgaaaacat ctgccaacag tggcaggtgc
    55021 cgctggtggt cgaacgtgtg cagcttgcgc aagaggggct aggtattgag gcccaggcgc
    55081 ggcaggcacg ttatcaggca tttgcccgca ccttgttgcc cggtgaagtg ctggttacag
    55141 cgcaacatct cgacgatcaa tgtgaaacct ttctgctggc gctaaaacgc ggtagcggcc
    55201 ctgccgggct ttcggctatg gcggaagttt cggagtttgc cggaacacgg cttatccgcc
    55261 cgttgcttgc ccgcacgcgg ggggaactgg agcagtgggc gttagcgcat ggtttacgct
    55321 ggattgaaga cgaaagtaat caggatgact catacgatcg taactttctg cgcctgcgcg
    55381 tagtgccatt attgcagcag cgttggccgc attttgccga agcaacagcc cgcagcgccg
    55441 cactttgcgc tgaacaagag agcctgttag atgaactgct ggcggatgat ttagcgcact
    55501 gccaaacgca acagggaacg ttaaagattg cgccaatggt ggcaatgagc gatgcccgtc
    55561 gcgcggcgat tatccgccgc tggctggcag ggcagaatgc accgatgcct tcccgcgacg
    55621 cgttggtgag gatctggcag gaagtggcgc tggcgcgaga agatgcttcc ccctgtttac
    55681 gtttgggtgc gtttgaaatc cgccgctatc agtcgcaact gtggtggatt aaatccgtca
    55741 ctgggcaaag cgaaaccatt ctgccgtggc agacgtggct acaaccgctg gaattaccgg
    55801 cggggctggg aagtgtacag cttactgcgg gaggcgatat tcgccctccg cgtgcagacg
    55861 aagcggtcag cgtgcgtttc aaagcgccag gattgctgca tattgtcggg cgcaacggcg
    55921 gacgtaagct gaagaaaatc tggcaagagt taggcgttcc gccgtggcta cgtgacacca
    55981 cgccactgct gttttatggc gaaacgctga ttgcggcggc aggggtattt gtgacgcaag
    56041 aaggtgtggc tgtaggtgag aatggcgtca gttttgtctg gcagaaaacg cttagtcaag
    56101 tgaaagccgg ataagacgca tcaaacgtcg catccggcga aagtcaatca ggactcgctc
    56161 accaccaccg taccgatttc cgggtggcta aagctggtaa ttttatccag acgcagctcg
    56221 cgggttgcac cagcggcctc gacgaccaga tactccacat ttttgcggga gactaaatcg
    56281 ctggccttcg cctgcaattt ttcgccatct ttcagctcaa gtgtcagcat taaatgatgc
    56341 tggcaggcga gctcaagatt atcgtaatca tcacaattga ttggttgata cgtatcattc
    56401 attgacataa tcgctcacca gtaagtttgc cgcagcgtat gctgcttttt ccctgacagc
    56461 ctcagaaagg gcgtcgtcgg cagccatttc attcagcact ttcaaaacgc agcccagcgc
    56521 gtccggaacg tatcctaagt ctccgctggc gatttccgcg taccgcttgc gtattaactc
    56581 acaatatttt tccacatgcc ctcctgtcag cactctgact taaccgtgga tgcaagtcta
    56641 agcctacgaa gataaactct gtttcgcaag gtgactatac cacactcatt tctgcaatat
    56701 cagcgccgca actgcacgta ttccgttaca atggcctcct gattcgaaag gagttttctt
    56761 atggcgctta aagcgacaat ttataaagcg acggttaatg tggccgatct cgaccgcaac
    56821 cagtttctcg atgcctctct gacgctggcg cgccatcctt cagaaaccca ggagcgcatg
    56881 atgctgcgcc tgctggcgtg gctaaaatat gccgatgaac gtctgcaatt tacccgtggt
    56941 ttgtgtgctg atgatgagcc ggaagcgtgg ctgcgtaacg atcatttggg tattgatttg
    57001 tggattgagc tggggctgcc ggatgagcgg cggattaaga aagcctgcac ccaggccgca
    57061 gaagtggcgc tgtttgccta taatagtcgg gcggcgcaaa tctggtggca gcaaaatcag
    57121 agcaaatgtg cgcagtttgc caatctttcc gtctggtatc tggacgacga gcaactggcg
    57181 aaagtgagcg cctttgccga tcgtactatg acgctgcagg caacgattca ggatggtgtt
    57241 atctggttat cggatgataa gaataatctg gaagtgaact taaccgtctg gcaacaacct
    57301 tcatgattgt gatttcccga catgttgcta tccctgatgg cgagtttgag atcaccgcca
    57361 ttcgtgcgca gggcgcgggc gggcagcatg ttaataagac ctcaacggct attcatctgc
    57421 gttttgacat tcgggcgtcc agcctgccag agtattacaa agagcgtctg ctcgccgcca
    57481 gccatcattt gatcagcagt gacggagtga ttgtcattaa ggcacaggaa taccgcagtc
    57541 aggaactgaa ccgcgaagcg gcgctggccc ggctggtggc agtgattaaa gatttaacaa
    57601 cagaacaaaa agcccgacga cctacgcggc ccacccgtgc atcaaaagag cgcaggctgg
    57661 catcgaaagc acaaaaatca agcgtgaagg cgatgcgcgg caaagtgcgc agcggtcggg
    57721 aataaaaaga aggaatggat ggtgaaaaaa gcgatagtga cagcgatggc tgtaatcagc
    57781 ctctttactc tgatgggatg taataatcgg gccgaagtcg atacgctttc tccggcgcag
    57841 gctgccgaac tgaaaccgat gccgcaaagt tggcgtggcg tgctgccgtg tgctgattgc
    57901 gaaggaatcg aaacctctct gttcctcgaa aaagacggaa cctgggtgat gaatgagcgt
    57961 tatctggggg ctcgtgaaga accttcctcc ttcgcttcct acggtacatg ggcgcgaacc
    58021 gctgacaagc tggtattaac cgatagcaaa ggtgaaaagt catattatcg cgcgaaagga
    58081 gatgcgctgg agatgctcga tcgtgaaggt aatccgattg aatcgcagtt caactacacg
    58141 ctggaaccgg cacaatccag cttacccatg acgccgatga ccctgcgggg catgtatttt
    58201 tatatggctg atgcggcgac cttcactgat tgcgcgaccg gaaaacgttt catggtggcg
    58261 aataacgcag agctggagcg tggctacctg gctgcgcgcg gtaacagtga aaaaccagtg
    58321 ttactgtcag ttgaaggtca ctttacgctg gaggctaatc ccgataccgg tgcgccgact
    58381 aaagtattag cgcccgatac ggcaggtaaa ttttacccaa accaggattg cagtagtttg
    58441 gggctgtaac ccgtcttgag acagaaacaa atgaaatatt gcctcatgcg ctgcgcttat
    58501 caggcctaaa gcatacaatg caatattttg aattagcacg attttgtagg ccggataagg
    58561 cgttcacgcc gcatccggca ttttgcgcac gaggctggct tttatcactg agtcagtcca
    58621 acaaagcgac ttgctttaat ataaatccct ggcttcaggt gcccaatata ctgtaactgc
    58681 gtttctggtt taaatgcaga gacatcacca ctacgcatat gcatcagatc ggcagggcta
    58741 atccagcctg attgcgccag cgtaagcgga tgcccggctt tggcaaatgc atcagtgaca
    58801 aattccgaac aaaaccacga ctttttgtct ccttcgccca cactgcttaa ctgcgctttc
    58861 gccaggccgc tgacgcactg ttgacgaaaa tcctcggaga acggatttag tgagcacatc
    58921 tggcgagtca ccataaatgg aataaattcg acaatgccgc gatagttgta accgctatct
    58981 ttaattttgt tggtgaacgc ggtgatttct gtggcttgtt gcggggtaag atccgggact
    59041 cgtaagacga aaagcttatc actatgcttt atggcttttt taagggaaac aatctggacg
    59101 ccagcgcctg tcgcttcggc aacgttatta tctcccaaat agattgcaac gtgactcaca
    59161 gaggaagtgc tgaatacgcg gataccaaat gaggttaccc caaggcttga ggagaaaagc
    59221 aaatcgccgg gtttgagatc tggttccgtt atttctttta ttgattgttc ggtgaaagag
    59281 ctttgatgct ggaatttaac agcccatgtt ttcgcctcgg catttaccac tgtggctgat
    59341 gagtctggct ggctgatatc aacggtacag gccgaaagta aaagaaagca ggggagaagc
    59401 aggcggcagt acgcctttgg tttatccatt ttatacaatc catgtaaaaa agggccccga
    59461 aattcaggac cctttctggc atcagccttt aatctgtttc accagataat cgacgatgtc
    59521 accagtctta attaactgtt tctcgccgtt acgacgatat ttatattcga tatcatcgtt
    59581 gtcgaggtta cggtcgccca gcacaatagt gtgcggaata ccgatcagtt ccatatcagc
    59641 aaacatcacg cccggacgct ctttgcggtc atccagcagc acttcgatac cttgtgcgcg
    59701 cagttcgctg tacagtttct cagcaagttc ctgtacgcgg aaggatttgt gcatgttcat
    59761 tggcagaatc gccacctgga acggcgcgat agcgtcaggc catacgatgc cgcgttcgtc
    59821 gtagttctgc tcaatcgccg cagctaccac acgcgttacg ccgataccgt agcaacccat
    59881 cgtcaggatt tggttacggc catcttcacc ctgtacggag gctttcagtg cttcggagta
    59941 cttggtaccc agctggaaga tgtgaccgac ttcgatacca cgtttgatca gcagcgtacc
    60001 ctgaccatcc gggcttggat cgccagccac cacgttgcgg atatcagcaa tttccggggt
    60061 agcgacatcg cgatcccagt tgataccgaa gtagtgttta ccatcgatgt tagcaccagc
    60121 agcgaaatca ctcatcgccg caacggtacg gtcaatcacc accggaatcg gcatgtttac
    60181 cggacccagt gaacccggac cggctttaac cacggcacga atttcttctt cggtcgcaaa
    60241 agtcagcggg ctggcaacct gcggcagttt ttctgcttta acttcgttca gctcgtggtc
    60301 accgcgcacc agcagcgcaa ccagcgggaa gctactgcct tcaacggctt taaccagcag
    60361 agtcttaacc gttttctcaa tcggcagatt gaactgttca accagttccg cgatggtctt
    60421 cgcgttcggc gtatcaacca gcgtcatttc ctgggtagca gcagcgcgcg gttctttcgg
    60481 cgcgatagct tctgccagtt caatgttcgc tgcatagtca gaagtatcgg agaagaccac
    60541 atcgtcttct ccgctctgcg ccagcacctg aaattcgtga gaggcgctac cgccgataga
    60601 accggtatcg gcctgtacgg cgcggaaatc cagccccatg cggctgaaga ttttgctgta
    60661 ggccgcatac attgcatcgt aggtttcctg cagggattcc tgagaagtat ggaaagagta
    60721 agcatctttc atcaggaatt cgcgggaacg catgacgccg aaacgcggac gcacttcgtc
    60781 gcggaacttg gtctggatct gatagaagtt cagcggcagc tgtttgtaag agctcagctc
    60841 gttacgaatc aggtctgtaa tcacttcttc atgagttggg ccgagtacga acggacggtc
    60901 accacggtca gcaatacgca gcaattccgg gccatactgt tcccaacgac cactctcttg
    60961 ccacagttca gatggctgaa ccaccggcat taacacctcg atcgcaccgg cgttgttcat
    61021 ctcttcacgc acgatgtttt cgactttttt cagaacgcgc acgccggtcg gcagccaggt
    61081 atataacccg gaggccagct tgcggatcat cccggcgcgc agcatcagct gatggctgat
    61141 cacctcggcg tcggcaggtg tctccttgag agtggagagc aggtattggc tagtacgcat
    61201 gttgttacgg ttccagttgg aaggtagaac aggctcaagg cgagcctggg acaaaaaaag
    61261 tgatttagtt taccagtgca aaagaaatgt caaaagagaa gggcgtgaat ttaacgcggt
    61321 tccagcgcaa agacttcaaa acctgcgtcg gtgacgcgcc agcgaacgtt aaaatcatgc
    61381 agccagacgg cataggtttt gcccgtttcc tcacctttac gataggccgg gcgcgggtcc
    61441 tgcgccagta cttcgcggat aaacagcgtt aactgcggat aacgcttctt cagcgtcaaa
    61501 agctgctttt cgacctctgc ggtaaaacac accgccattt ctgccgctgg cgcactttgc
    61561 gcatagctgg cactggcatc gggaagcgat tcagcaaagg ggagatacgg tttgatatcc
    61621 actaccggcg taccatcgac cagatccaga ctgccgagct tcagaatcac gctgtcttta
    61681 tggcaaacaa cctctttcag ctctaccagc gacataccaa tcgggttagg gcgaaaagtg
    61741 gagcgcgtag cgaaaacacc cattctggca ttaccgccga gacgaggcgg acgcacagtc
    61801 ggacgccagc cgccttccat cgtttgatga aagacgaaaa ggatccataa atggctgaat
    61861 gcttccaggc cgcgaacggc gtcggcctgg ttgtagggag caatgagatg tagttcgccg
    61921 ttggcgcttt ttaccagacc tggctggcgc ggaacggcga atttttcttt atagggcgag
    61981 cgaataacgc ctatttgctc gaactggaaa ctgctcattt cgccgtgatg ttaagcgcag
    62041 aaccgataca tacagcctga cgatagcaac ctggcgtacc gctggtgact tcgcagctat
    62101 gcagtaatac cgcattggct ttcattttcg aggcgttgat ttgcatccgc ttacgtgcgg
    62161 ttggaatgct cggcggagag tcctgattag aggcctggca agagtcgcca ctgacttcac
    62221 cgagatcgcg gaacggtttg ccgactaatt cttctgcatt ggtataaatt cggaccggcg
    62281 tggcgcgcgg cgctttcggt tttgcaggct ccgctttcgg ctggggtgca gtgctttgaa
    62341 cgggttcgac aggggatctg cttaacatgg aacagccgct tagcatgagt gctactaaac
    62401 agatcggtaa agcacgcata gtatttcctc aatgtatgat caaaacgtca atattgaatc
    62461 aggcgcttgt aaaaatgaca agacgggcaa gcgcccgtcc tgaatgatat tacaaattgt
    62521 ggaaacagcc taaatattac cagcctttaa cagcgccgcc gttaaacact ttgtttgctg
    62581 cttcgtaaac ttcgtcagac tgataagcct gaacgaattt cttcacgttt tcggcgtctt
    62641 tgttgtcttc acgcgttacg atcaggttta cgtacgggga ctctttatct tcgacaaaga
    62701 taccgtcttt cgctggagtc aggccaatct ggctggcata ggtggtattg ataactgcca
    62761 gagcgatttg cgcgtcgtcc agagagcgcg gcagctgcgg tgcttccagt tcaacaattt
    62821 tcagattttt tgggttctca acaacatcaa gaacggtcgg cagcaggcca acgccatctt
    62881 tcagtttgat caagcccact ttttgcagca gcagcagtga acgaccaagg ttagttgggt
    62941 cgtttggcac ggcaacctgc gaaccatcct gcagttcatc cagtgatttg attttcttgg
    63001 agtaaccagc aatcggataa acaaatgtgt tgcctactgc gaccagtttg tagccacgat
    63061 ctttcagttg ctgatcaagg tacggtttat gctggaaggc gttggcgtcg atatcgcctt
    63121 tgctcaatgc ttcgtttggc agaacatagt cgttgaaggt taccagctca acgtccaggc
    63181 catatttgtc tttcgcaact ttctgcgcga cttctgcaac ctgctgttcg gcaccaacaa
    63241 tcacgccgac tttaatgtgg tttggatctt tttcatcctg accgcagcct accagtgcca
    63301 gtgatccaat cagggctccc actgccgcaa aggttttgaa tttgaacgcc ataccttatt
    63361 ccttcttcaa ttatttatgt tgtgttgaac gttacttgcg agtgacagcc cggacgatgc
    63421 ggtcgcctgc gaactgaatt aaataaacca gaatgaccag caataccagt accgtattca
    63481 tcaccgtcgc gttgtagccg atgtagccat actgatagcc aatctgacct aaaccaccgg
    63541 caccgactgc accacccatc gcggaataac caaccagggt aatcagggta atagttgccg
    63601 catttaccag acccggcaac gcttccggta acagcacttt acggacgatc tgcattggcg
    63661 tggcacccat tgcgcgggaa gcttcaatta acccggttgg gatctccagc agagcattct
    63721 cgaccatacg ggcaataaac ggtgctgcac caacggttaa cggaacaatc gctgcctgca
    63781 aaccaatcga tgtaccaaca ataacgcggg taaacggaat catccatacc agcaagataa
    63841 tgaacgggat ggaacggaaa atgttcacaa tcgcagaaac ggtacgatac agcttcgcgt
    63901 tagcaataat ttgccccgga cgcgtgacat aaagcaggac gccaaccggc agaccaatca
    63961 caaatccaaa aaaaccggat acgaaggtca ttgtcagcgt ttcccatacg ccacgaacca
    64021 gcagccacat catcggctca gacataaccc agtacctcta cttttacatg gtgttcctgc
    64081 agccaggtaa tggcggcttg cgtatcttgt tgtgtgccgt gcatttcagt cagcatgatg
    64141 ccgaacttaa cgccaccggc gtaatccatc tgcgcgctaa taatgttgtt gttgacgttg
    64201 aaacgacgcg cggtttcaga aagcagtggg gcatcgaccg attgaccggt aaactccaga
    64261 cgcaacatcg gcacgcagtc agtgaatggc tccgcttgca gacgttcctg gtaatcttcc
    64321 gggatatcca gatgcagggt cgactgaata aacttctgcg ccagcggcgt tttcggatgc
    64381 gagaacactt cacttaccgt gtcctgctcg atcagttcgc cattgctgat gaccgccacg
    64441 caatcacaaa tacgcttcac aacgtccatt tcgtgggtga tcaacagaat cgtcaaaccc
    64501 agacggcggt tgatgtcttt cagcagttcg agaatagaac gtgtcgttgc cggatccagc
    64561 gcgctggtgg cctcatcaca cagcaatact ttgggattgc tggctaacgc acgggcaatc
    64621 gccacacgct gtttctgccc accggaaaga ttcgacgggt agctatcatg cttatcgcca
    64681 agaccaacca atgacagcaa ttccgtcacg cgacgtttga tctcatcttt cggtgtgttg
    64741 tccagctcca gcggcagagc cacgttgcca aaaacagtac gcgaagagag caggttaaaa
    64801 tgctggaaaa tcataccaat ctggcggcga gttttggtca actcggattc tgacagcgtg
    64861 gtcagttcct ggccatcgac cagcacgcta ccctcggttg ggcgctccag caggtttaca
    64921 caacgtataa gcgtactctt acccgcgcca gaggcaccga taacgccata aatttgtcca
    64981 gctggcacat gcaggctgac gttgttcaac gcctggatgg tgcgggtgcc ctggtggaac
    65041 actttggtga tattcgaaag ttttatcatt gattatttat tatcgtcatt aagttagtcg
    65101 tggcatttcg aatgcctgga acgggcaacg ccttcaatga aatggatgtt aaggcatcca
    65161 gacgtctaaa tcaatcaggt ttatgcgaag agcactttct tgctggtcga aacatgcgat
    65221 actagcgtca catgccttat taaggagcta taaaaggtgg cgaagagcgt acccgcaatt
    65281 tttcttgacc gtgatggcac cattaatgtc gatcacggct atgtccatga gatcgacaac
    65341 tttgaattta tcgacggtgt tattgacgcc atgcgcgagc taaaaaaaat gggctttgcg
    65401 ctggtggtag taaccaacca gtctggcatt gctcgcggta aatttactga agcacagttt
    65461 gaaacgctga ccgagtggat ggactggtcg ctggcggacc gcgatgtcga tctggatggt
    65521 atctattatt gcccgcatca tccgcagggt agtgttgaag agtttcgcca ggtctgcgac
    65581 tgccgcaaac cacatccggg gatgcttttg tcagcacgcg attatttgca tattgatatg
    65641 gccgcttctt atatggtggg cgataaatta gaagatatgc aggcagcagc tgcggcgaac
    65701 gtgggaacaa aagtgctggt gcgtacgggt aaacctgtta cgcctgaagc agaaaacgcg
    65761 gcggattggg tgttaaatag cctggcagac ctgccgcaag cgataaaaaa gcagcaaaaa
    65821 ccggcgtaat gattaaaaga tgagcggttg aaataaaaat gcatttttcc gcttgtcttc
    65881 ctgagccgac accctataat gcgcctccat cgacacggcg gatgtgaatc acttcacaca
    65941 aacagccggt tcggttgaag agaaaaatcc tgaaattcag ggttgactct gaaagaggaa
    66001 agcgtaatat acgccacctc gcgacagtga gctgaaagcc gcgtcgcaac tgctctttaa
    66061 caatttatca gacaatctgt gtgggcactc gaagatacgg attcttgacg tcgcaagacg
    66121 aaaaatgaat accaagtctc aagagtgaac acgtaattca ttacgaagtt taattctttg
    66181 agcatcaaac ttttaaattg aagagtttga tcatggctca gattgaacgc tggcggcagg
    66241 cctaacacat gcaagtcgaa cggtaacagg aagcagcttg ctgctttgct gacgagtggc
    66301 ggacgggtga gtaatgtctg ggaaactgcc tgatggaggg ggataactac tggaaacggt
    66361 agctaatacc gcataacgtc gcaagaccaa agagggggac cttcgggcct cttgccatcg
    66421 gatgtgccca gatgggatta gctagtaggt ggggtaa
//
LOCUS       DAPHCV010000008        63334 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.008, whole genome shotgun sequence.
ACCESSION   DAPHCV010000008 DAPHCV010000000
VERSION     DAPHCV010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.008"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            277..456
                     /locus_tag="RH033_000528"
     CDS             277..456
                     /locus_tag="RH033_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDV2989766.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            568..1041
                     /locus_tag="RH033_000529"
     CDS             568..1041
                     /locus_tag="RH033_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001665680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HDV2989767.1"
                     /translation="MTHLLSDTYSAEKQLTRALAKLARATSSEKLSQAFHAHLEETHG
                     QIERIDQVMESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRGAALIAAAQKVEH
                     YEIASYGTLSTLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNKKAENKA"
     gene            1087..1593
                     /locus_tag="RH033_000530"
     CDS             1087..1593
                     /locus_tag="RH033_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HDV2989768.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPIIEAILNDEKHMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(1652..2290)
                     /gene="ompW"
                     /locus_tag="RH033_000531"
     CDS             complement(1652..2290)
                     /gene="ompW"
                     /locus_tag="RH033_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HDV2989769.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKETGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            2530..2619
                     /locus_tag="RH033_000532"
                     /pseudo
     CDS             2530..2619
                     /locus_tag="RH033_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            2647..3390
                     /locus_tag="RH033_000533"
     CDS             2647..3390
                     /locus_tag="RH033_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HDV2989770.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            3420..3959
                     /locus_tag="RH033_000534"
     CDS             3420..3959
                     /locus_tag="RH033_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HDV2989771.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            4064..4462
                     /gene="yciA"
                     /locus_tag="RH033_000535"
     CDS             4064..4462
                     /gene="yciA"
                     /locus_tag="RH033_000535"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HDV2989772.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(4502..5221)
                     /gene="tonB"
                     /locus_tag="RH033_000536"
     CDS             complement(4502..5221)
                     /gene="tonB"
                     /locus_tag="RH033_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HDV2989773.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            5445..5741
                     /locus_tag="RH033_000537"
     CDS             5445..5741
                     /locus_tag="RH033_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HDV2989774.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            6042..7295
                     /gene="kch"
                     /locus_tag="RH033_000538"
     CDS             6042..7295
                     /gene="kch"
                     /locus_tag="RH033_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HDV2989775.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(7350..7523)
                     /locus_tag="RH033_000539"
     CDS             complement(7350..7523)
                     /locus_tag="RH033_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HDV2989776.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            7666..9126
                     /gene="cls"
                     /locus_tag="RH033_000540"
     CDS             7666..9126
                     /gene="cls"
                     /locus_tag="RH033_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDV2989777.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            9161..9490
                     /locus_tag="RH033_000541"
     CDS             9161..9490
                     /locus_tag="RH033_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HDV2989778.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(9543..10547)
                     /gene="oppF"
                     /locus_tag="RH033_000542"
     CDS             complement(9543..10547)
                     /gene="oppF"
                     /locus_tag="RH033_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HDV2989779.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(10544..11557)
                     /gene="oppD"
                     /locus_tag="RH033_000543"
     CDS             complement(10544..11557)
                     /gene="oppD"
                     /locus_tag="RH033_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HDV2989780.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNRLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(11569..12477)
                     /gene="oppC"
                     /locus_tag="RH033_000544"
     CDS             complement(11569..12477)
                     /gene="oppC"
                     /locus_tag="RH033_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HDV2989781.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(12492..13412)
                     /gene="oppB"
                     /locus_tag="RH033_000545"
     CDS             complement(12492..13412)
                     /gene="oppB"
                     /locus_tag="RH033_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HDV2989782.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(13498..15129)
                     /gene="oppA"
                     /locus_tag="RH033_000546"
     CDS             complement(13498..15129)
                     /gene="oppA"
                     /locus_tag="RH033_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HDV2989783.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIITNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKATDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            15413..15709
                     /locus_tag="RH033_000547"
     CDS             15413..15709
                     /locus_tag="RH033_000547"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2989784.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSLFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            complement(15867..16514)
                     /gene="ychE"
                     /locus_tag="RH033_000548"
     CDS             complement(15867..16514)
                     /gene="ychE"
                     /locus_tag="RH033_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HDV2989785.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVTIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            16991..19666
                     /gene="adhE"
                     /locus_tag="RH033_000549"
                     /gene_synonym="adhC"
     CDS             16991..19666
                     /gene="adhE"
                     /locus_tag="RH033_000549"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDV2989786.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            complement(19968..20585)
                     /gene="tdk"
                     /locus_tag="RH033_000550"
     CDS             complement(19968..20585)
                     /gene="tdk"
                     /locus_tag="RH033_000550"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDV2989787.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALEVGSLTAIQERHRHD"
     gene            21189..21602
                     /gene="hns"
                     /locus_tag="RH033_000551"
     CDS             21189..21602
                     /gene="hns"
                     /locus_tag="RH033_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HDV2989788.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(21747..22655)
                     /gene="galU"
                     /locus_tag="RH033_000552"
     CDS             complement(21747..22655)
                     /gene="galU"
                     /locus_tag="RH033_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDV2989789.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(22857..23870)
                     /gene="rssB"
                     /locus_tag="RH033_000553"
     CDS             complement(22857..23870)
                     /gene="rssB"
                     /locus_tag="RH033_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HDV2989790.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(23962..24867)
                     /gene="rssA"
                     /locus_tag="RH033_000554"
     CDS             complement(23962..24867)
                     /gene="rssA"
                     /locus_tag="RH033_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HDV2989791.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            24980..25438
                     /locus_tag="RH033_000555"
     CDS             24980..25438
                     /locus_tag="RH033_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HDV2989792.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMK
                     DADYLIKTWHPSCGAATLRAELIAGFAHTEWLGLTVFEHCWQDVDNIGFVSFVARFTE
                     GSKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            25488..26330
                     /gene="purU"
                     /locus_tag="RH033_000556"
     CDS             25488..26330
                     /gene="purU"
                     /locus_tag="RH033_000556"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HDV2989793.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHERLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            26490..26574
                     /locus_tag="RH033_000557"
     tRNA            26490..26574
                     /locus_tag="RH033_000557"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26524..26526,aa:Tyr,seq:gta)
     gene            26622..26752
                     /locus_tag="RH033_000558"
     ncRNA           26622..26752
                     /ncRNA_class="other"
                     /locus_tag="RH033_000558"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(26936..27613)
                     /gene="narI"
                     /locus_tag="RH033_000559"
     CDS             complement(26936..27613)
                     /gene="narI"
                     /locus_tag="RH033_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDV2989794.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(27613..28323)
                     /gene="narJ"
                     /locus_tag="RH033_000560"
     CDS             complement(27613..28323)
                     /gene="narJ"
                     /locus_tag="RH033_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDV2989795.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFAEKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(28320..29858)
                     /gene="narH"
                     /locus_tag="RH033_000561"
     CDS             complement(28320..29858)
                     /gene="narH"
                     /locus_tag="RH033_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDV2989796.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(29855..33598)
                     /locus_tag="RH033_000562"
     CDS             complement(29855..33598)
                     /locus_tag="RH033_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HDV2989797.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAIGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TADELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAREAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(33991..35382)
                     /gene="narK"
                     /locus_tag="RH033_000563"
     CDS             complement(33991..35382)
                     /gene="narK"
                     /locus_tag="RH033_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HDV2989798.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            35721..37517
                     /gene="narX"
                     /locus_tag="RH033_000564"
     CDS             35721..37517
                     /gene="narX"
                     /locus_tag="RH033_000564"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HDV2989799.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADTHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWVQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            37510..38160
                     /gene="narL"
                     /locus_tag="RH033_000565"
     CDS             37510..38160
                     /gene="narL"
                     /locus_tag="RH033_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HDV2989800.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(38161..39555)
                     /gene="ychO"
                     /locus_tag="RH033_000566"
     CDS             complement(38161..39555)
                     /gene="ychO"
                     /locus_tag="RH033_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HDV2989801.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDAFSQQVNQHV
                     ESWLSAWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGSWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHFNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVVESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSEEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            39741..40094
                     /gene="ychN"
                     /locus_tag="RH033_000567"
     CDS             39741..40094
                     /gene="ychN"
                     /locus_tag="RH033_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HDV2989802.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(40138..40833)
                     /gene="chaC"
                     /locus_tag="RH033_000568"
     CDS             complement(40138..40833)
                     /gene="chaC"
                     /locus_tag="RH033_000568"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HDV2989803.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPGWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGLA"
     gene            complement(40991..41221)
                     /gene="chaB"
                     /locus_tag="RH033_000569"
     CDS             complement(40991..41221)
                     /gene="chaB"
                     /locus_tag="RH033_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HDV2989804.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            41491..42591
                     /gene="chaA"
                     /locus_tag="RH033_000570"
     CDS             41491..42591
                     /gene="chaA"
                     /locus_tag="RH033_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HDV2989805.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIGINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            42997..43104
                     /locus_tag="RH033_000571"
     CDS             42997..43104
                     /locus_tag="RH033_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDV2989806.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAVIVSWWRNRK"
     gene            complement(43253..44107)
                     /gene="kdsA"
                     /locus_tag="RH033_000572"
     CDS             complement(43253..44107)
                     /gene="kdsA"
                     /locus_tag="RH033_000572"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HDV2989807.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(44143..44952)
                     /locus_tag="RH033_000573"
     CDS             complement(44143..44952)
                     /locus_tag="RH033_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HDV2989808.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVTL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(44956..45348)
                     /locus_tag="RH033_000574"
     CDS             complement(44956..45348)
                     /locus_tag="RH033_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HDV2989809.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(45345..46178)
                     /gene="prmC"
                     /locus_tag="RH033_000575"
     CDS             complement(45345..46178)
                     /gene="prmC"
                     /locus_tag="RH033_000575"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDV2989810.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALACERPDCEITAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDDQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(46178..47260)
                     /gene="prfA"
                     /locus_tag="RH033_000576"
     CDS             complement(46178..47260)
                     /gene="prfA"
                     /locus_tag="RH033_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDV2989811.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(47302..48558)
                     /gene="hemA"
                     /locus_tag="RH033_000577"
     CDS             complement(47302..48558)
                     /gene="hemA"
                     /locus_tag="RH033_000577"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HDV2989812.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEERDDLQEALIRWLCDYHNLNEDDLRNSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQEASEFMA
                     WLRAQSASETIRDYRSQAEQVRDELTAKALAALEQGGDAQTIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            48772..49395
                     /gene="lolB"
                     /locus_tag="RH033_000578"
     CDS             48772..49395
                     /gene="lolB"
                     /locus_tag="RH033_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HDV2989813.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTSDDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            49395..50246
                     /gene="ispE"
                     /locus_tag="RH033_000579"
     CDS             49395..50246
                     /gene="ispE"
                     /locus_tag="RH033_000579"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDV2989814.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLSTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            50397..51344
                     /gene="prs"
                     /locus_tag="RH033_000580"
     CDS             50397..51344
                     /gene="prs"
                     /locus_tag="RH033_000580"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDV2989815.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            51469..53148
                     /gene="dauA"
                     /locus_tag="RH033_000581"
     CDS             51469..53148
                     /gene="dauA"
                     /locus_tag="RH033_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HDV2989816.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPDSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNLEFQPLRTMARAGIQPILGRLAFFPNRRAAMADL"
     gene            complement(53203..53481)
                     /gene="ychH"
                     /locus_tag="RH033_000582"
     CDS             complement(53203..53481)
                     /gene="ychH"
                     /locus_tag="RH033_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HDV2989817.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            53759..54343
                     /gene="pth"
                     /locus_tag="RH033_000583"
     CDS             53759..54343
                     /gene="pth"
                     /locus_tag="RH033_000583"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDV2989818.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            54460..55551
                     /gene="ychF"
                     /locus_tag="RH033_000584"
     CDS             54460..55551
                     /gene="ychF"
                     /locus_tag="RH033_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDV2989819.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAVRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            55743..55919
                     /locus_tag="RH033_000585"
     CDS             55743..55919
                     /locus_tag="RH033_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2989820.1"
                     /translation="MKKTFTFSLLAIVFASLLSACVPHHHHRHSDGPRGPAPSGKPMP
                     PSNGGPNHGSHGRY"
     gene            complement(55956..57875)
                     /gene="dhaR"
                     /locus_tag="RH033_000586"
     CDS             complement(55956..57875)
                     /gene="dhaR"
                     /locus_tag="RH033_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001467850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HDV2989821.1"
                     /translation="MSSAFNNDGRDISPLIATSWERCIKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRACALLILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLHSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRG"
     gene            58103..59173
                     /gene="dhaK"
                     /locus_tag="RH033_000587"
     CDS             58103..59173
                     /gene="dhaK"
                     /locus_tag="RH033_000587"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDV2989822.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEESQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            59184..59816
                     /gene="dhaL"
                     /locus_tag="RH033_000588"
     CDS             59184..59816
                     /gene="dhaL"
                     /locus_tag="RH033_000588"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HDV2989823.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSIPAALEAASS
                     VAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            59827..61245
                     /gene="dhaM"
                     /locus_tag="RH033_000589"
     CDS             59827..61245
                     /gene="dhaM"
                     /locus_tag="RH033_000589"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HDV2989824.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDNCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADTDHVLVMMDIGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGAEIDKVIFDAMHALEAKREQLGLPSSNTEISDTCPSYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPILRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVDEEQERLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTILQLDPAVVKGICLSAGSPLSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFSRQG"
     gene            61566..63263
                     /gene="treA"
                     /locus_tag="RH033_000590"
     CDS             61566..63263
                     /gene="treA"
                     /locus_tag="RH033_000590"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HDV2989825.1"
                     /translation="MKSPAASRPLKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPKPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILSRASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRASKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPLSESTTQPLKQKEAEPTP"
BASE COUNT        15963 a        16184 c        15334 g        15853 t
ORIGIN      
        1 ctgtcgaacg tcaacttacg tcatttttcc gcccaacagt aatataatca aacaaattaa
       61 tcccgcaaca taacaccagt aaaatcaata attttctcta agtcgcttat tcctcaggca
      121 attgttaata tatccagaat gttcctcaaa atatattttc cctctttctt ctcgttgcgc
      181 ttaatttgac taattttcat tagcgactaa ttttaatgag tgtcgacaca caacactcat
      241 attaatgaaa caatgcaacg caacgggaga aataacatgg ccgaacatcg tggtggttca
      301 ggaaatttcg ccgaagatcg tgagaaggca tccgacgcag gccgtaaagg cggtcagcat
      361 agcggcggta attttaaaaa tgatccgcaa cgcgcatctg aagcgggtaa aaaaggcggt
      421 caacaaagcg gtggtaataa atcaggaaaa tcctgattca ccgttaatta ttaatatcag
      481 ctgataaaga taattgcatc tgcgggccgt atggctcgca gtgatttcac tggagaaaaa
      541 tatgaatatg aagaccattg aagatgtatt actcacctgc tttcagatac ctacagcgca
      601 gaaaaacaat taacccgtgc actggcaaaa ctcgctagag caacatcaag cgaaaaatta
      661 agtcaggctt ttcatgcgca cctcgaggaa actcatggac agattgaacg aattgatcaa
      721 gttatggaat ctgaatcgaa cctgaaaatt aagcgcatga aatgtgtggc aatggaaggt
      781 ctcattgaag aagctaatga ggtcatcgaa agcaccgaga aaaacgaagt gcgtggtgcc
      841 gcactgattg ccgctgcaca gaaagtcgag cactatgaga tcgccagtta cgggacttta
      901 tcgacgctgg ctgaacaatt aggttatcgt aaagcagcga agcttctgaa agagaccctg
      961 gaagaagaaa aggccaccga catcaaattg actgatctgg cccttaataa cgtaaataag
     1021 aaagccgaaa ataaagcctg aaatatgaac tttaactttt agtcatttta taaagaggac
     1081 attttcatga atcgtattga acattatcat gactggttac gtgacgccca cgcaatggaa
     1141 aagcaagccg aatctatgct tgagtccatg gccagtcgta tagataatta tcctgaacta
     1201 cgcgctcgta ttgaacaaca tcttagtgaa accaaaaacc agattgttca actggaaact
     1261 attcttgatc gtaatgacat ttcacgttca gtcattaaag attccatgag taaaatggct
     1321 gcacttgggc agtcaatcgg tggtatattc ccttctgatg aaatagtcaa aggctctatt
     1381 agcggatatg tcttcgagca atttgaaatc gcctgttata cctcactctt agcagcagca
     1441 aaaaatgccg gtgatacagc ctcaattccg attatcgaag cgattttaaa tgatgaaaag
     1501 cacatggccg actggctaat tcagcatatt ccgcaaacaa ctgagaaatt cttaattcgc
     1561 tctgaaactg atggcgtaga agcgaagaaa taataccctt tataccatgt ccttattgac
     1621 cccgtaatta cggggtcatt tttgtgcgga attaaaaacg atatcctgct gagaacataa
     1681 acacccacgg atcgaggcgt acgctatcgt gttgctgtgc accgcccagt ttataattgg
     1741 cagtggtatc aatatccatg taccagactg acatgttaac caaccagtca cggttaatca
     1801 gataatcaac ccccacctgc ccggcagctc cccaggaatc tttcagactg agatcggaaa
     1861 gccccgtctc tttgccatga tcgttaaatc cattatcaaa gaaggtggtg tagttaatac
     1921 ctgccccaac gtaaggacgg aatttgctgc tggcatcacc aaaataccac tgcgccatca
     1981 gtgttggcgg cagatgatga acggttgcaa tatcgccggt cgcccgggtg ccgattttat
     2041 ggcggaacgg cgtcgctgcc agcaattcca caccaatgtt atcggtcgcc atataagtaa
     2101 acgtcaggcc cagttgcgta ttattggtca cgctgaatcc acccagactt cctaacgtac
     2161 caccagcacc ttctgttgga cgtacggttg cagaacctgc acgcataaaa aattcgcctg
     2221 cttcatgcgc aaaggcactg ccagagagaa gagttgttac tgccaaagcc gccactgtta
     2281 actttttcat atccgctccg tcgttatggt tataaaaacg tgacgaatat acctacattg
     2341 gagtaacaaa tgatcctaca tagatcacat tacatatgat aattcaccat ttattgatct
     2401 ggattaattt agaagttgca ttgagaatcc tggttaatgt gatttaaatc aatttttaaa
     2461 tattttcgct acgcaaatat taactcgatc gccacttgcc gcgatttgcc cacgtctgaa
     2521 ggccagcaat tacctccgct ttacaaagat atgcttttcc atacaagacc tgatgctgcc
     2581 acccgaggct taaccagggt acaattgctc gctaattaac acctgcataa aactcaagga
     2641 gagtgcatgt ctatcacggc gcagtccgta taccgtgaca ccggaaattt cttccgtaat
     2701 caatttatga ccattctgtt ggtatcgttg ctatgtgcgt ttatcacagt ggtgttaggg
     2761 catgttttct cacccagtga tgcacagctt gcgcagctca atgacggcgt gcccgttagc
     2821 ggcagtagtg ggttgttcga cctggttcag aatatgtcac cggaacagca acaaattttg
     2881 ctgcaggctt cagcggcgtc cactttttca ggattaatcg gtaacgccat tctcgccgga
     2941 ggcgtaatat taattatcca gctggtgtct gcgggtcaga gagtcagtgc gctgcgagct
     3001 attggtgcca gtgcgccgat attgccaaag ttatttattc tgatttttct gactaccctt
     3061 ttagtacaga ttggcatcat gctggtggtc gttccgggaa ttatcatggc cattttactg
     3121 gccctggcac cggtgatgtt ggtacaggac aaaatgggcg tttttgcctc gatgcgtagc
     3181 agtatgcggc tgacttgggc gaatatgcgt ctggtggcac ccgcagtgct gagctggttg
     3241 ctggcaaaga cactgttgct gctttttgcc tcttcttttg ccgcattaac cccggaaatt
     3301 ggtgccgtac tggcgaacac cttgagcaac ctgatttcag ccgtactgct catctatctg
     3361 ttccgcctgt atatgttgat tcgccaataa ccttaagcat ttgattacgg aatcgtaaaa
     3421 tgaagcagtt tcttgatttt ttaccgctgg ttgtcttttt cgcgttttac aagatttatg
     3481 acatctatgc ggctactgcg gcgctgatcg tcgccacggc gattgtgctt atatatagct
     3541 gggttcgctt tcgtaaggtt gagaagatgg ccctgatcac ttttgttctg gtggtcgtct
     3601 tcggtggctt gaccctgttc ttccacaatg atgagtttat taaatggaag gttactgtca
     3661 tttatgccct gtttgcgggt gccctgttag tcagccaatg ggtgatgaaa aagccgctaa
     3721 ttcagcggat gctgggtaaa gaactcacgc tgccgcaacc ggtatggtcg aagctgaatc
     3781 tggcctgggc tgttttcttt atcctttgcg gtctggcaaa catctacatc gcattctggc
     3841 tgccgcaaaa tatttgggtc aactttaaag tctttggcct gaccgccctt accttaatct
     3901 tcacattgtt aagcggtatc tatatctacc gccacatgcc gcaggaagat aaatcctaac
     3961 cagactgcca gtccttcggg gctggcgctg tatctcctct gttctcctca ggaaaatcat
     4021 agtagcatcg cgcctgtgat tttcctttta agtcggtttt accatgtcta caacacataa
     4081 cgtccctcag ggcgatcttg ttttacgtac tttagccatg cccgccgata ccaatgccaa
     4141 tggtgacatc tttggtggtt ggttaatgtc acaaatggat attggcggcg ctattctggc
     4201 gaaagaaatt gcccacggtc gcgtagtgac cgtgcgggtt gaaggaatga ctttcttacg
     4261 accggttgcg gtcggcgatg tggtgtgctg ctatgcacgc tgtgtccaga aagggacgac
     4321 atcggttagc attaatattg aagtgtgggt gaaaaaagtc gcgtctgaac ccatcgggca
     4381 acgctataaa gcgacagaag cattatttaa gtatgtcgcg gttgatcctg aaggaaaacc
     4441 tcgcgcctta cctgttgagt aataatcaaa agcctccggt cggaggcttt tgactttctg
     4501 cttactgaat ttcggtggtg ccgttaattt taaacaggat gttcaccaca atcccactgc
     4561 ctggcttacc cggctcataa cgccatctgc gcatcgcatt tttcacctca cgctcaaaca
     4621 tgttcgcagg cttggctgag aggatttgta cgttatccac gcgaccatcc ggcgtgacat
     4681 caaatttaac tttaacctgc ccttcaatgc gcaatgcctg tgctcgtgcc ggatactgcg
     4741 gctgattgcg gcttaatgcg cgtggtcctg aagccacact ggtaaccggc ttgctggttg
     4801 cagccgttgc cgtacttgat gtagggcgcg cgggtgcagt attttcaaac ggtgatgccg
     4861 gacgcgactc tacgggtttg acatcgcgtt ttggctgctc ctgtaccttt ttcaccggct
     4921 tcggttttgg cttaggtttc ggcttcggct tttcaatgac caccggtgct tcttttggcg
     4981 gttcggggat cggctcaggt tccggttctg gctctaccac cggctccggt ggcggctgaa
     5041 cggcttgtgg cggttcgaga tccgcaggcg caaccatcgt gacagaaatt ggctgcgcag
     5101 gcgcaggtag ttcaataacc tgatgtaccg aggtatagag cagacccgcc acaacagcac
     5161 catgaatgca gaccgaaagt aacgtcggcc aggggaagcg gcgaggtaaa tcaagggtca
     5221 ttgaagtcat aatcatttca gtagaaaaac caggcctcga ttttaaatgc aaatagcaat
     5281 catattcaat aaggcaagac gatcaggatc agtgaaaatg tcatttttgg cgtaattgac
     5341 cttacttttt aaatggtctt aacaaagaca tcatctttcc gttgcagatc ttcgcccttt
     5401 caaataacgt actttacaac tttcccgaac aaggagttgt gcccgtgttg tatgttattt
     5461 acgctcaaga caaagctgat agcctcgaaa aacgcctttc cgttcgtccg gcacatttag
     5521 cacgtttaca gttactgcat gatgaaggtc gattgttgac cgccggtcca atgccagcag
     5581 tagacagtaa cgatccaggg gctgcgggat ttactggctc aaccgttatt gctgaatttg
     5641 aatcgctgga agcagctcag gcctgggccg atgctgaccc gtacgtagcg gcaggagtct
     5701 atgaacacgt ttcagtgaaa ccatttaaga aagtgttctg aatagaaatt caatatttaa
     5761 gggcacggtt ttagcaaccg tgttcttgtt tggcctttcc ttataggtca gtcattgcat
     5821 ttaccaccac aagcagataa taactttatc tgtatttctt tatatgatgt gagcatattc
     5881 aggaaaaatg ccctttcctg acgcagagtt gctcactcaa aacctttgat tactgcggat
     5941 aaagtcatct actattgttt aacacattca cgcgctgcat gtatgttcag aaaatgtgca
     6001 cgctaagata attaaacaaa attgtggtta cagaggtatc agtgagtcac tgggctacat
     6061 tcaaacaaac agcaactaat ttatgggtga ccctacggca cgacattctc gcgctggccg
     6121 tctttttaaa tggattgctt atttttaaaa caatctatgg tatgtcggtc aatttgctag
     6181 atattttcca tatcaaagca ttttcagagt tggatctctc cttgctggca aacgccccac
     6241 tctttatgct cggcgtcttt cttgtcctga actccattgg cttactgttc cgggcaaagc
     6301 tcgcatgggc aatcagtatc attttgttgt tgatagcgct aatttacacc ctgcattttt
     6361 atccctggct gaaatttagt attggatttt gcatttttac gctggtgttt ttgctgatac
     6421 tgcgcaaaga cttctcccac agtagcgccg cagccgggac aattttcgca tttattagtt
     6481 tcacgacgtt actgttttac tccacctacg gtgcgcttta tttaagcgaa ggttttaatc
     6541 cgcgaataga aagtttgatg accgcgttct atttttcgat agaaaccatg tcaaccgtcg
     6601 gctatggcga tattgtccct gtttctgaat cagcacgatt gttcactatt tcggtcatta
     6661 tttccggcat taccgttttt gccacatcta tgacctcaat ttttggtccg cttatccgcg
     6721 ggggattcaa caaacttgta aaaggaaaca atcatacaat gcatcgtaaa gatcatttta
     6781 ttgtttgcgg acattcgatt ctcgccatca atacgatcct gcaactgaat caacgcggac
     6841 aaaacgtaac ggttatcagc aacttgcctg aagatgatat caagcaactt gagcaacgct
     6901 taggcgataa cgctgatgtt atccccggtg acagtaatga cagttcagta ttaaagaaag
     6961 caggaattga tcgatgccgg gccattctgg cgctgagtga taacgatgca gataacgcgt
     7021 ttgttgtact ctcggcaaaa gatatgagca gtgatgtcaa aacagttctc gccgtcagtg
     7081 atagcaaaaa cctgaataag attaagatgg tacatccgga tatcattctc tcgccgcaac
     7141 tgtttggcag cgaaattctg gcgcgagttt taaatggtga agagattaat aatgatatgc
     7201 tcgtttcaat gttgttgaac tccggtcatg gcattttcag cgataacgat gaacaagaaa
     7261 cgaaagctga cagtaaagaa tcggcgcaaa aatagagttt aaagagggaa gagtaaactt
     7321 attgggcacc gcaatggtgc ccagtacatt tagatattgt agatcgcaaa caataacgag
     7381 ttacgctgtt tgtttagaat gcacttcctg atgctgtgga tacgcatatt tcggcgcgac
     7441 tgcccctcaa gccaacgcga tttacgtcgg ctagcctgac gctgcatgcg ccagcgcccc
     7501 acttccgttc tactccgctt catgttaact actctatgca ataacagaat ggtcattata
     7561 cccccgcccc cgacgctgac cagtgctttt cccgcgtttt tatttgccag atgaatccat
     7621 atgcgtaaac tcataacaat gcgctttcaa aaggatttct aatctatgac aaccgtttat
     7681 acgttggtga gttggttggc cattctggga tactggttgc tcattgcagg cgtaacttta
     7741 cgcattctaa tgaaacgacg cgcagttccc tccgcgatgg cctggctgtt gattatttac
     7801 attctgccgt tagtcggaat tattgcctat cttgccgttg gcgagctcca tttaggcaaa
     7861 cgccgcgctg agcgcgccag agcgatgtgg ccttccaccg caaaatggct taacgacctt
     7921 aaagcctgta agcatatctt cgccgaagaa aatagcagtg tcgctgcgcc attattcaag
     7981 ctttgcgagc gtcgtcaggg gatcgctggg gtcaaaggga atcagctgca actgatgacc
     8041 gagtcagatg atgtgatgca ggcgttaatc cgcgacatcc agctcgctcg ccataatatt
     8101 gagatggtgt tttatatctg gcagcccggc ggcatggcgg accaggtggc tgaatcatta
     8161 atggcggctg cgcgacgcgg cattcattgc cgattgatgc tcgactccgc cgggagtgtg
     8221 gcttttttcc gcagcccgtg gcccgagcta atgcgtaatg ccggtattga agttgtcgaa
     8281 gccttaaagg tcaatctgat gcgtgtgttt ttacgccgta tggacctgcg ccaacatcgc
     8341 aagatgatca tgatcgataa ttacatcgcg tacaccggca gcatgaatat ggtcgaccct
     8401 cgctacttca aacaagatgc gggcgtaggg caatggattg atctgatggc gcgtatggaa
     8461 ggtcccatcg ctaccgcgat ggggattatt tattcctgcg actgggagat tgaaaccgga
     8521 aaacgtattc tgccgccacc accagatgtc aatattatgc cgtttgagca ggccagcggt
     8581 cacaccattc acacaattgc ttctggcccc ggctttccgg aagatctcat tcaccaggca
     8641 ttattgactg ctgcttattc ggcgcgtgaa tatttgatca tgacaacgcc ctactttgtg
     8701 cccagcgatg atttacttca tgcgatttgc acggcggcgc agcgcggggt ggatgtcagt
     8761 attatccttc cgcgaaaaaa tgactcgatg ctggtcggct gggccagtcg cgcattcttt
     8821 acggaactgc tggctgctgg ggttaaaatt tatcagtttg aaggcgggtt actgcatacc
     8881 aagagcgtgc tggtcgatgg tgaactaagt ctggttggca ccgttaacct tgatatgcgg
     8941 agtctgtggc taaatttcga gattaccctg gcaatcgacg ataaaggttt tggtgcagac
     9001 cttgccgccg ttcaggacga ttatatttcg cgttcacgtc tgctcgatgc ccgtttatgg
     9061 ctaaaacgtc cattatggca acgtgtcgcc gagcgactgt tttacttctt cagcccgttg
     9121 ctgtaaaacg tcgccaacag aggttaaaca ggtagtgact atggatatgg atctaaacaa
     9181 tcgcctgact gaagatgaaa cgcttgagca ggcctacgat atttttctcg aactggctgc
     9241 cgacaatctc gatccagctg atgttctgct gttcaatctt cagtttgaag agcgcggcgg
     9301 cgcggagtta ttcgatccgg cggaagactg gcaggaacat gttgatttcg atttgaaccc
     9361 ggactttttt gccgaagtgg tgattggtct ggcggacagt gaagatggtg aaatcaacga
     9421 tgttttcgcg cgcattctgt tatgccgcga aaaagatcac aaactttgcc atatcatctg
     9481 gcgggaataa ataatcgctt ttggcgtgaa taagggctga caactgtcag cccttattgc
     9541 ttttaaagcg gatcgacttt caggcaagaa acggcgtgtc tgaaactccc ttccagcaca
     9601 ggacgtgttt tggcgcactc cggaccggca atcggacaac gggtacggaa aacacaaccg
     9661 gaaggcgggt tgatcggcga cggtaattcc ccttccagta actggatggt tttgttcttc
     9721 tccagatccg gatcaggtat ggggactgcc gacatcaatg ccctggtgta aggatgtagc
     9781 ggattgtggt agacctcatc ataggtcccc agttctaccg catggccgag atacatcacc
     9841 agcacacgat cggaaatgtg ttttaccacg gccaggtcgt gagcgataaa aattaacgac
     9901 aatcccatct cacgttgcaa ctgctggagc aggttgacca cctgcgcctg aattgacacg
     9961 tccagcgccg acaccggctc atcacagata atcagcttcg gttcaagaat aagagcacga
    10021 gcaatcccga tacgctggca ctgcccaccg gagaactcat gcggatagcg gttaatcaga
    10081 ttaggcaaca aaccgacttt cagcatcatc gccttcacgc gctcgcgaac ttcctggcgt
    10141 gacattttcg gatgataagt acgcagtggt tcagcgatga tctcgccgat ggtcatacgc
    10201 gggttaagcg atgccaacgg atcctggaaa atcatctgaa tatcactgcg aacggcacgc
    10261 cattcatcgg gcttcatgcc cagcaactct ttacctaacc aggcaacatg accgtcggtc
    10321 gccttgacca aaccgatgat ggcgcgagca aaggtggact taccgcatcc cgattcccct
    10381 accacaccta atgtttcccc ttcatacagg cgaagagtga caccatctac ggctttgagc
    10441 gttttcggag gttgccagaa ccactgtttg ccatctttga tttcaaagtg cactttcaga
    10501 tcggcgattt cgaggaggac ttttcttcct tcggttacag cattcataac agttcctcca
    10561 ccggtttaaa gcaagcacgc agacggccag gcgtaaactc ttccagcggc ggtgcgctac
    10621 tacaaatttc catcgcatgc ggacaacgtg gctggaacgg gcaacctttc ggtaatcgca
    10681 gcaggtttgg cggattacca gggatggtca acattgtttc accttccgca tcgagacgcg
    10741 gcaccgcgtt gagcaaaccg atagaataag gatgaacggg ttgataaaag acatcgcgcg
    10801 cgttgccata ttccatcgtg cgcccggcgt acattaccag cactttgtcg cagatccccg
    10861 ccaccacgcc aagatcgtgg gtaatcataa tgatggcggt attaaattcc cgcttcagtt
    10921 cattcaatag cgtcatgatc tgcgcctgta cggtgacgtc cagcgcagta gtgggttcat
    10981 ccgcaatcag cagcttaggt cgacatagca atgccatcgc aatcataact cgctgacgca
    11041 tgccaccaga aaattcgtgc gggtacattt tcatgcgttt acgcgcttcc ggcattttta
    11101 ccgcatcgag catccgcacc gactcttcaa acgcctcagc tttgctcatg ttcttatgca
    11161 gcatcagcac ttccatcaac tgctcaccga cgcgcatata gggattcaac gaagtcattg
    11221 ggtcctgaaa aatcattgag atttgttcag cgcgcagtct attgagttca cgctctggca
    11281 aattgaggat ttcacgccca ttgaaggttg ccgatccgcc aatacgtccg ttggcagcca
    11341 gcaggcccat caacgcaaat gcagtttgcg atttacccga accagactca cctacaatgc
    11401 ccagcgtttc tccggcacgt agggaaaaat tcaaatcatt gaccgccgtg acgtcgccgt
    11461 ccggggtact aaaggtgaca cgcaaatctt tcacgttcag cagtgcgtca gcctgttgtt
    11521 gtgcgagcgg cacagttgca gtttcaatta cgctcatggc tgcactcctt aacgatcttt
    11581 cgggtcgagg gcatcacgca agccatcgcc tataaagttg aaacaaaata gcgtcaccac
    11641 gaggaatccc gctgggaaca acagtaacca tggagagact tccatcgagt tcgcgccatc
    11701 actcagcaat gcgccccagc tgcttaacgg ctcttgcgtc cccaacccca ggaagctaag
    11761 gaaagattca aagaggatca tgctgggcac cagaagtgat gcgtagacca ccaccacacc
    11821 gagtacattc ggcacaatgt ggcgaataac aatgcccgac gtcgatacac cgccaacttg
    11881 tgccgcctca ataaactctt tgcgcttcag actcagggtt tgcccacgca caatacgagc
    11941 catatccagc caggaaacca tgccaatcgc cacgaaaatc agcaggatgt tttgaccgaa
    12001 aaaggtcacc agcaaaatga cgaagaacat gaatgggaag gagttgagga tttccagcag
    12061 acgcatcatt accgaatcca ctttaccgcc cagataaccg gaaagcgaac cataaagtgt
    12121 ccccacgacc actgccacca gtgccgcagc aacaccgacc atgagtgaga tacgcccgcc
    12181 aatcgcaaca cgcacaagca ggtcgcgacc ggatgagtca gtaccaaagt agtgaccgga
    12241 ctccatatcc ggggcgctgg acatcatcgc ccagtcagta tcgtcatagg caaactgcga
    12301 aagcatcggt gccaggatta caaataacgc gattagcacc agcactatca gactggcaac
    12361 cgccgcacgg ttatgcataa aacgtcgacg tgcgtcctgc cacaagctgc gcccttcgac
    12421 ctccagcttt tcactgaaat tttccagcgt ctcgctgttt ttcttactta acatcatcgc
    12481 gagctccagt atcagtaacg gattttcggg tcgataaccg catatagcac atcgacaatg
    12541 gcattaaaca aaatggttaa cgcaccaacc aggatagtca ggcttaacac taaggaatag
    12601 tcacggttca atgcgccatt aacgaacagt tgcccaatcc ccggcaaacc ataaatggtt
    12661 tcgatgacca tagaaccggt aataatgccg acaaatgcgg ggcccatata ggagagcacg
    12721 ggtaacagag caggttttaa tgcgtggcgt aaaatgatcc gccgcatagg taaccctttc
    12781 gcccgggcag tacgaataaa gttggagtgt aatacttcaa tcatagagcc acgggtaata
    12841 cgcgcaatac tggcgatata ggcgagtgac aacgccacca ttggcagtat catgaattta
    12901 agcgcaccac cattccagcc accgcccggc agccaatgca aaatgatcgc aaatatcatg
    12961 actaataatg gcgcaaccac aaaactgggg ataacaaccc cggtcattgc cagccccatc
    13021 acggtatagt cccatttggt gttttgtttt aatgcggcaa taacgccagc actaacaccc
    13081 agtattaccg caaggaaaaa tgctgcggct cccagtttgg cagaaacggg aaaactggat
    13141 gcaaccaggt cgttgaccga ataatcttta tatttaaacg atggaccgaa atcgccatgc
    13201 gccagttgtt tcaggtagct gaaatactgt gtcatgatcg gatcattaag atgatatttc
    13261 gcttcgatat tggccatcac ttctggcggt aaagtacgtt cgccggtaaa agggcttccc
    13321 ggcgcgaggc gcatcatgaa gaacgaaata gtaataagaa taaatagcgt cggaatcgct
    13381 tccagacagc gacgtagaat aaattttaac attgccctac cttctggcct gtgccttctg
    13441 taatgcgata aaaatcagac accgtggagc agtacacgcc tgccccacgt attgccatta
    13501 gtgcttcaca atgtacatat tccgggtgta ggtattatcc agcggatctt tgccggtata
    13561 gccaccaacc cacggtttca ccagacgcgc attcacgtag taataaacag gaacaatggc
    13621 cgaatcctta tccagctgtt gttctgcttt agtgtacaga gctgtgcgct gcgcctcgtc
    13681 agtcgctttc agcgtttccg ccataatgct gtcaaaggcc gggctcttat aatgcgcggt
    13741 attcattgag ctgttcgaca gcatggtgtt caggaaggaa gttggttcgt tgtagtctgc
    13801 acaccagcct gcacgggcca catcaaaagt gccctggtga cgggtgtcga ggaacgtttt
    13861 ccactcctgg ttaaccagtt tgacgtttac accaatgttt ttcttccaca atgaagaggc
    13921 agcaatcgcc agctttttat gcagatcgga ggtgttatac aacaggttga tggtcaacgg
    13981 tttgtctgcg gtataacccg cttcagccag cagttttttc gcttcttcgt tacgtttttc
    14041 ctggctccag ccaaaccatt ccggctgagt caattttgcg ccatcagtat acggtggagt
    14101 gtaaccatag gcgggcatgt cgccctgcgc tttcacttta ttaacaatga tatcgcggtc
    14161 catacccagt ttcagtgcgg tacgcacacg tacatcgttg aatggcggtt tctggttgtt
    14221 aatttcgtaa taataagtgc acaggtatgg atcaacgtga acttcgtccg ggatctcttt
    14281 tttcagcttc tggaacaatt cgatcggcat gttgttatac gtcatgtcga tttcaccact
    14341 acggtagcgg ttgacatcgg taacttcaga agcaataggc aaataggtta cctggttaat
    14401 aacggttttc gcgttgttcc agtaggttgg gctgcgttca agaacgattc gttcgtttac
    14461 gacccaatct tttaaggtat aggcaccgtt ggtgatgata ttaccaggct gggtccattt
    14521 ttcgccgaat ttctcgatag cggcttttgg caccggtgaa gttgatgggt gaacaagtaa
    14581 tttatagaag tacggaacag gttcacttaa ggtgacttct aatgtgtgat catcaattgc
    14641 tttcacgccg agatcggtaa tcggtttttt cccttcaaga atttcatcaa taccggcgat
    14701 atgcccatat tgcagataac tggcatacgg agaagcggtg tttggatcaa cagaacgttg
    14761 ccagctatac acaaagtctt gtgctgtgac tggcgtgcca tcagaccatt tcgcatcttt
    14821 acgcaaatgg aaggtccaga ctttcgcgtc tttattatcc caggattcag cgacgccagg
    14881 tgccggatga ccgtcaagat cgctgaccag taagccttca aacaggtctc ggctgatatt
    14941 agactccgga acaccttcaa ttttatgcgg atctaatgac tgaacttctg aaccattgtt
    15001 acgtaccagc gtttgttttt ccgccagtgt gacgcctgcg ggtacatcag ctgccagcgc
    15061 gacattccct gccattagtg cagccagaac gccagctgct actaaacttc tcttggtgat
    15121 gttggtcatt gttttttgga ctccctcatt ataattactg gtattaacca gcatgtgtaa
    15181 tcccggattg ggaccctgca caggctcgga gactttctgc tgtcaggtta ctttagcgtt
    15241 acggattgct atcaccgact ttatttattc tggtgattca gacgatcacc ttatgtcgat
    15301 tcgtactgtg tctcccctgt cgagacacat tattctttcg cgacgctctt taattgttaa
    15361 ttattctcat taccaataaa gaatcgcctg gcggttgccc ggaaagtacc aaatgcgctt
    15421 cttactcgcc aatacatttt gcaaaattgt taagcaattc tcttttattg accgcatcag
    15481 aatctatagc ttgcatccat gtcttaacaa acatctttca attcaatcaa atagagattg
    15541 gttaagcatg tctctctttt ctgattacag ttccagtagt gaaatgcaca ataatttaac
    15601 aattgattat tatttagcac tttcctctac caaaggcagt ggaattacta atatcatttc
    15661 aattattctt cagcaagccc aggattatga tgtggcgaaa ataacatgat ggtgaggaga
    15721 agcagttaag gaaatcctta taactaactg taatttgaga agaatatttt cacccattcc
    15781 ttttgctttt gataactgcg cgggaacaaa gtaaaaataa tgacgaagta aacggagcat
    15841 gacagctccg tttcatttga aaggaattaa ttaagcaggc cggggaaaat ccccttaata
    15901 ccagtaacga taaattcaat ccccaatgcc atcagcaata gccccataat acgcgtaatc
    15961 acgttgatgc cggtctggcg taaaacccgt accagccacg gtgccatgcg gaacaatccc
    16021 cagcaacata aagcgaacaa tgcaatagtc acaaagaaac caaacagata gctaatgctg
    16081 tgataacgcg tcccccagac gatggtagaa ctgatcgccc ctggccccgc catcaacggc
    16141 aacgccaatg gcaccacacc aatgctttca cgcaccgcgg tttctgattt ttcttgcttg
    16201 ttctgtttat cctcgccaag cttgccgctg atcatcgaca tcgctatcgt caccaccagg
    16261 atgccaccgg cgatacggaa tgaatcaatt gatataccaa aaagttgtag aatcgtgtcg
    16321 ccgagaaaaa gcgagatcca caagataatg gccacagaca ggttggctgt aaggttcgtt
    16381 ttgtttcgcg ctgccgctgt ctgataactg gtcatgctga taaagacggg aataatccct
    16441 accgggttga ccagcgcaaa taacccgatg aaaaatttga agtaaacggg aaaatcaaaa
    16501 agggtctgaa tcacggttag ctccgaagca aaagccggat aatgttagcc ataaataagg
    16561 ttaaaaagac gcgctgacaa tacgcctttt gacagcattt ttcacctcct aactacttaa
    16621 aattgctatc attcgttatt gttatctggt tgtgcaaaac atgctaatgc agccaacaaa
    16681 tcatactaca atttattaac tgttagctat aatggcgaaa agcgatgctg aaaggtgtca
    16741 gctttgcaaa aatttgattt ggatcacgta atcagtaccc agaagtgagt aatcttgctt
    16801 acgccacctg gaagtgacgc attagagata ataactctaa tgtttaaact cttttagtaa
    16861 atcacagtga gtgtgagcgc gagtaagctt ttgattttca tcggttaagc aaatcatcac
    16921 cgcactgact atactctcgt attcgagcag atgatttact aaaaaagttt aacattatca
    16981 ggagagcatt atggctgtta ctaatgtcgc tgaacttaac gcactcgtag agcgtgtaaa
    17041 aaaagcccag cgtgaatatg ccagtttcac tcaagagcaa gtagacaaaa tcttccgcgc
    17101 cgccgctctg gctgctgcag atgctcgaat cccactcgcg aaaatggccg ttgccgaatc
    17161 cggcatgggt atcgtcgaag ataaagtgat caaaaaccac tttgcttctg aatatatcta
    17221 taacgcctat aaagatgaaa aaacctgtgg tgttctgtct gaagacgaca cttttggtac
    17281 catcactatc gctgaaccaa tcggtattat ttgcggtatc gttccgacca ctaacccgac
    17341 ttcaactgct atcttcaaat cgctgatcag cctgaagacc cgtaacgcca ttatcttctc
    17401 cccgcacccg cgtgcaaaag atgctaccaa caaagcggct gatatcgttc tgcaggctgc
    17461 tatcgctgcc ggtgctccga aagatctgat cggctggatc gatcaacctt ctgttgaact
    17521 gtctaacgca ctgatgcacc acccagacat caacctgatc ctcgcgactg gtggtccggg
    17581 catggttaaa gccgcataca gctccggtaa accagctatc ggcgtaggcg cgggcaacac
    17641 tccagttgtt atcgatgaaa ctgctgatat caaacgtgca gttgcatctg tactgatgtc
    17701 caaaaccttc gacaacggcg taatctgtgc ttctgaacag tctgttgttg ttgttgactc
    17761 tgtttatgac gcagtacgtg aacgtttcgc aacccacggc ggctatctgt tgcagggtaa
    17821 agagctgaaa gctgttcagg acgttatcct gaaaaacggt gcgctgaacg cggctatcgt
    17881 tggtcagcca gcctataaaa ttgctgaact ggcaggcttc tctgtaccag aaaacaccaa
    17941 gattctgatc ggtgaagtga ccgttgttga tgaaagcgaa ccgttcgcac atgaaaaact
    18001 gtccccgact ctggcaatgt accgtgctaa agatttcgaa gacgcggtag aaaaagcaga
    18061 gaaactggtt gctatgggcg gtatcggtca tacctcttgc ctgtacactg accaggataa
    18121 ccaaccggct cgcgtttctt acttcggtca gaaaatgaaa acggctcgta tcctgattaa
    18181 caccccggct tctcagggtg gtatcggtga cctgtataac ttcaaactcg caccttccct
    18241 gactctgggt tgtggttcct ggggtggtaa ctccatctct gaaaacgttg gtccgaaaca
    18301 cctgatcaac aagaaaaccg ttgctaagcg agctgaaaac atgttgtggc acaaacttcc
    18361 gaaatctatc tacttccgcc gtggctccct gccaatcgcg ctggatgaag tgattactga
    18421 tggccacaaa cgtgcgctca tcgtgactga ccgcttcctg ttcaacaatg gctatgctga
    18481 tcagatcact tccgtattga aagcagcagg cgttgaaact gaagtcttct tcgaagtaga
    18541 agctgacccg accctgagca tcgttcgtaa aggtgcagaa ctggcaaact ccttcaaacc
    18601 agacgtgatt atcgcgctgg gtggtggttc cccgatggac gctgcgaaga tcatgtgggt
    18661 tatgtacgaa catccggaaa ctcacttcga agaactggcg ctgcgcttta tggatatccg
    18721 taaacgtatc tacaagttcc cgaaaatggg tgtgaaagcg aaaatgatcg ctgtcaccac
    18781 cacttctggt acaggttctg aagtcactcc gtttgcggtt gtaactgacg acgctactgg
    18841 tcagaaatat ccgctggcag actatgcgct gaccccggat atggcgattg tcgatgccaa
    18901 cctggttatg gacatgccga agtccctgtg tgctttcggt ggtctggacg cagtaactca
    18961 cgccatggaa gcttatgttt ctgtactggc atctgagttc tctgatggtc aggctctgca
    19021 ggcgctgaaa ctgctgaaag aatatctgcc agcgtcctac cacgaaggat ctaaaaatcc
    19081 ggtagcgcgt gaacgtgttc acagtgcagc aactatcgcg ggtatcgcgt ttgcgaacgc
    19141 cttcctgggt gtatgtcact caatggcgca caaactgggt tcccagttcc atattccgca
    19201 cggtctggca aacgccctgc tgatttgtaa cgttattcgc tacaacgcga acgacaaccc
    19261 gaccaagcag actgcattca gccagtatga ccgtccgcaa gctcgccgtc gttatgctga
    19321 aattgctgac cacctgggtc tgagcgcacc gggcgaccgt actgctgcta agatcgagaa
    19381 actgctggca tggctggaaa cgctgaaagc tgaactgggt attccgaaat ctatccgtga
    19441 agctggcgtt caggaagcag acttcctggc gaacgtggat aaactgtctg aagatgcatt
    19501 cgatgaccag tgcaccggcg ctaacccgcg ttacccactg atctccgagc tgaaacagat
    19561 tctgctggat acctactacg gtcgtgatta tgtagaaggt gaaactgcag cgaaaaaaga
    19621 agccgctccg gctaaagctg agaaaaaagc gaagaaatcc gcttaatcag tagcgctgtc
    19681 tggcaacata aacggtccct tctggggccg ttttttgttt tacccaaagc aactttccca
    19741 taaaccgaca gcatttgcct tcatcatatt tacggcgatg tataacgcct acacacagcg
    19801 atattgtact ttacaggtca caagtcaacg tcactgtaaa agcgctctgt gaggggtata
    19861 gtggcgttaa agaactgtcg agaagggtat atacaccgga tgaagtgagt aaaacgaact
    19921 gacaggataa aagcgccctg ctcaccctgt cagtaaagaa attcttatta atcgtggcga
    19981 tgcctttcct gaatagccgt taatgagccg acttctaacg cttctttata gtgtttacgg
    20041 catacagaaa cgtagcgttc attaccacca ataaccacct gctcaccttc gttataaggt
    20101 ctgcctgctt gatcaagacg cagcaccatg cttgctttgc ggccacaaaa acagatggtt
    20161 tttaattcaa ccagtttgtc ggaccatgcc agtaagtatt ggctgccaat aaataactca
    20221 cctcgaaaat cggtacgtaa accataacaa agtacgggta tatcgagctg atcgacaacc
    20281 tccgataatt catatacttg ttgtctggtt aaaaactggc attcatcaac cagtacgcaa
    20341 tgaattgcct gctgttcatg ttccgcacga atctcatcaa ataatgatga attttggtta
    20401 aataattttg caggcgatga caaacctata cgcgaactga ctttcccggc accaaagcga
    20461 tcgtcaattt ctgccgtata tacgacagtg cgcatgccgc gttcctggta attgtatgaa
    20521 gattgcaaca atgctgtaga cttacccgca ttcattgcgg aatagtagaa atatagctgt
    20581 gccatcagcc acaggccctc aatgatatga attaaagttg gctggagttt atcataattc
    20641 gtcagttttt cagctcaaca gtatgcacag aagtggtata tgcgttctcc cttacgaagc
    20701 cttgcataat ccttctgagc tatcattaca actgcctcgc ttgttataag cgggtaaatg
    20761 actgctggta actattcaca atctttaacc cgttgcgcaa gtaatagccc tctgttgacc
    20821 tcctggagat agtgcaatac taagtccctg ctcttattgc gactgttcta cttttcatca
    20881 ttcgcttaat agggaattct cgtaaacacg actaatacag aagactgaaa ggtcgtcagc
    20941 ctacgataat ctccccataa aatgtgacat gaatcaggaa gttttaacct cacgtgctgc
    21001 gaaatcatcg gtgcaaatag ggctatatgc cgcgtctttt ctggctaatt ttatgaaaag
    21061 atatttattg gcggcacaaa ataaagaaca attttgaatt ccttacattc ctggctattg
    21121 cacaactgaa tttaaggctc tattattacc tcaacaaacc accccaatat aagtttgaga
    21181 ttactacaat gagcgaagca cttaaaattc tgaacaacat ccgtactctt cgtgcgcagg
    21241 caagagaatg tacacttgaa acgctggaag aaatgctgga aaaattagaa gttgttgtta
    21301 acgaacgtcg cgaagaagaa agcgcggctg ctgctgaagt tgaagagcgc actcgtaaac
    21361 tgcagcaata tcgcgaaatg ctgatcgctg acggtattga cccgaacgaa ctgctgaata
    21421 gccttgctgc cgttaaatct ggcaccaaag ctaaacgtgc tcagcgtccg gcaaaatata
    21481 gctacgttga cgaaaacggc gaaactaaaa cctggactgg ccagggccgt actccagctg
    21541 taatcaaaaa agcaatggat gagcaaggta aatccctcga cgatttcctg atcaagcaat
    21601 aatcttttgt agattgcact tgcttaaaaa tcccgccatc ggcgggattt tttattgtcc
    21661 ggtttaagac aatttaataa gaataagcta taaaaaaacg gcgtcgattg ctcaacgccg
    21721 tttcgtggat aacaccgata cggatgttac ttcttaatgc ccatctcttc ttcaagccag
    21781 gctttaaatt ccgtgccaag ggtgttatga cgaataccgt attcaacgaa ggcctgcatg
    21841 taacctaatt tattaccgca gtcatggctc ttccctttca tatgataggc ttccaccgtt
    21901 tctttttcga tcagcatatc aattgcgtcg gtgagctgaa tttcatcacc agctcccgga
    21961 ggggtttttg ccagcaacgg ccaaatatcc gcgctaagta cgtaacgccc cacaatagcg
    22021 agattagacg gcgcaacatc cgcttttggt ttttcaacca cgccaaccat cggtacgctt
    22081 tcacccggtg ctaattcaac gcctttgcaa tccacaacgc catatgcggt cacatcagca
    22141 accggttcaa ccatgatctg gctatgaccc gtttcatcaa agcggcggat catctctgcc
    22201 aggttatcct gtgacaaatc ggattcatat tcatccagaa taacgtcagg caaaataaca
    22261 gccacaggtt catcacccac taccgggtga gcacacaata ccgcgtgtcc caggcctttc
    22321 gccagcccct gacgaacttg cataatagtc acgtgcggcg gacaaataga ctgcacttca
    22381 tcaagcagtt gacgttttac acgtttttcc agcattgctt ccagttcaaa actggtatca
    22441 aagtggtttt caatagagtt tttagatgag tgagtaacca gcacaatttc agtaatgcca
    22501 gccgcaatac attcattcac gacgtattga attaatggct tatcgacaag tggcagcatc
    22561 tctttcggga tggctttcgt cgccggcaac atcctggttc ctaatcccgc aacggggata
    22621 acggcttttt tgactttcgt attaatggca gccatttaaa ttctcctgga ctgttcgtgt
    22681 tttgaacgtg ttcatatttc tgtatcgcat cccagtatat cagcaccccg aaggggctaa
    22741 gttctgaaaa ggaggcgagt tagcataatt aagacaatta cttatacgaa tggcgacgat
    22801 agtaccacca gccgacatta gcaggtaatg caaatttagc ccgcgttatc gtttgctcat
    22861 tctgcagaca acatcaagcg cagtcgacca ccggttcccc atatttggca ttgccaggca
    22921 tcgcatcgct ggctcaattg attcaaataa gcgttaccta aagtgcctaa cggaacacca
    22981 ttactgattt gtacctggtg ttcgccggta tttaacgtcg cattcagacc cgcagaaacc
    23041 agaatgagat ttttcagttc gcgatgataa tagccaacta atagcggaaa ttgccccggc
    23101 agattggcct gacggagtaa atggtttacc tgcttcaata acgcgcccaa ctctggcaac
    23161 cgttgatttt ggtgtgcaag ctgttcctgt aataatccgt taaacaatgc gcgtaataac
    23221 aaggcagcaa gtacgccatt atgtccagct cgggtgacat caaggcaata aaatgccaga
    23281 tcgttttccg aaagtgcggc aatatcaagc accaggccgg gtttgtccgc ggcaaccaat
    23341 tgacgataat taacccggca atgggaaatc acctgctgaa ccggcggttg tagttcctgt
    23401 aataatttcg ccgctgcggc agggttatca accattgcat cccagtcgcg aaaaagcctt
    23461 tcctcttctt caacgcggga attaaacatg ctgggataga gacaggcaaa aaccatctcg
    23521 cgcaagcgat tcagatcttt aactggtttc agcaaaacat cttcaacgcc cagacgtaac
    23581 gctttggcaa tatctgccat attttcagtg gcagatatca ccagaactgg ggtctggtcg
    23641 cctctgttac gtatatgctc cagcagttta agcccgttca ttcgtggcat cgcgatatca
    23701 catatcatca ggtctggagt gaaacctccc agcaactcaa gggcatccac cccatcagcc
    23761 gccagtaccg ttgtcgctcc caatgaggaa aaccacgaat ccagaagcga gcgaaatacc
    23821 tgctcatctt caacaatgag aatctgtttt ccgaccaatg gctgcgtcat gttctctccc
    23881 ctgactggct ttactcaata gtggcatgct attgccacct gcgcctgtca gaattaactt
    23941 aagtgtagag aaaaattctg gtcaaatgtt ggtgcgtacc aacggcaaaa gttcgtccat
    24001 tttcttttcc actgccagct gcccggccgc aatggcagcg tgcgcgcgat ggaaatcaag
    24061 cgtagatatt tgcgggcaaa caggttgaat cagaatatcg ggcggatcac ctgccatgcg
    24121 gttcctttta aggcggttct ccagcacctg gatagaagtg gtcataatct ctgttgccgt
    24181 tggcgctgtc accgcccgac gtgtcgttat gctgcccaac ctttctttca gacgcgcatg
    24241 ccacggcaga gaatcaccat tctcgctatt ttcttcactg acattaaagg agagcagatc
    24301 ttgttgcatc aaatgagcat cgtgctgcag gtcgaccgct atcacaatat cagcacccaa
    24361 tgcacgcgtg agcgaaatag gaattgggtt aactacagcg ccatcaacca gccagtagcc
    24421 gttatgcgca acgggtgcca ttagtcctgg aatactgcat gatgcacgaa tagcaagatg
    24481 gagatcgcct tcagtaaacc ataattcgcg cccagtactt aaattggtgg caacagccgc
    24541 gaagcgacgg gaacaatttt cgatctctgt ttccggcatt atttcgcgat attgattgaa
    24601 gacacgctcg ccgcgcagta acccaccgcg ctgccaggag agatccatca ggcgtaaaac
    24661 atcccaataa ctgaaagagg tcacccaatc ttccagcgca gatagtcgat cgcatgcata
    24721 ggcagcgccc accagcgaac caattgaaca tcctgcaacg atatcaattt caatacccac
    24781 tttttttagc gcattaataa cgccaatatg cgaccaacct ctcgccgcgc cagatcccag
    24841 cgccagccct atttttatct ttctcattat tcccgcaaga ttcccctgga atgcaatcgt
    24901 agcaacatcg ctactgctcg gttaacatag tgctactcag gcggttaata cgccgttatt
    24961 gtttccaggg agattatttg tgtctcagct ttgtccctgt ggtagtgctg tcgagtatag
    25021 cctatgttgc cacccttatg tgtctggtga aaaggttgca cctgatccag aacatctcat
    25081 gcgttcgcgt tactgcgctt ttgtgatgaa agacgcagat tatttaataa agacctggca
    25141 tccatcttgt ggagcggcga cgttacgtgc cgaattgatt gccggatttg cgcatactga
    25201 atggttggga ttgaccgtat ttgaacattg ttggcaggac gtcgacaata tcggatttgt
    25261 cagttttgtt gcgcgattta ccgaaggtag caaaacaggt gcaataattg aacgttcgcg
    25321 atttttgaaa gaaaacggtc aatggtacta tattgacggt acacgtccgc agtttggccg
    25381 caacgatccc tgcccttgtg gttcaggtaa aaaatttaaa aagtgctgcg gccaataatg
    25441 gttgacggta cggtttagca aacactctca acaaggtttt tccagcaatg cattcactcc
    25501 aacgtaaagt tctgcgtact atttgtccgg accaaaaagg tctgatcgca cgtattacca
    25561 atatttgcta caagcacgag ttaaatatcg tacagaacaa tgaatttgtt gatcaccgta
    25621 ccgggcgctt ttttatgcgc acggaactgg aaggaatttt taatgattcc accctgctgg
    25681 cggatctcga cagcgcattg ccagaaggct ccgtacgtga gctgaatcct gccggtcgtc
    25741 gccggatagt gattctggtc actaaagagg cgcattgcct tggcgatttg ttgatgaaag
    25801 ccaattacgg cggcctggat gtcgaaatcg cggcagttat tggtaaccac gatactttac
    25861 gttctctggt tgagcgtttt gatattccgt ttgagctggt aagccatgaa aggttaagcc
    25921 gcaacgagca cgatcaaaag atggcggatg ccattgatgc ttatcagcct gactacgtgg
    25981 tgctggcgaa gtatatgcgg gtattaacgc cagaatttgt ggcacgcttc ccgaataaga
    26041 tcatcaatat tcaccattca ttcctgccag cgtttatcgg cgcacgtcct tatcaccagg
    26101 cctatgaacg tggcgtgaag attattggcg ctaccgctca ctatgtgaat gacaatctgg
    26161 acgaaggtcc aatcatcatg caggacgtta ttcatgtcga tcatacctac acagctgaag
    26221 atatgatgcg cgcaggtcgt gacgtcgaga aaaacgtctt aagtcgcgca ctctacaaag
    26281 tactggcgca gcgcgtcttt gtttacggta atcggacgat tattctttaa tcgccagcaa
    26341 aaataactgg ttacctttaa tccgttacgg gtgaaaatta cgcaaccagt tcatttttct
    26401 caacgtaaca ctttacagcg gcgcgtcatt tgatatgatg cgccccgctt cccgataagg
    26461 gagcaggcca gtaaaaagca ttaccccgtg gtggggttcc cgagcggcca aagggagcag
    26521 actgtaaatc tgccgtcatc gacttcgaag gttcgaatcc ttcccccacc accatcactt
    26581 ttcaaaagtc cgtgaactct caaacgaatc cgcaattaaa tattctgccc atacggggag
    26641 ggatgagaag cttcgtccaa ggtgcgactc gaacgccagc gagaaagcgt tgccgcaggc
    26701 aacgacccga agggtgaagc gcgcagcgct agtaatcctt cccccaccac cattacttcc
    26761 tgaaatactc aaaacctgtt actccgagct aattctgccc gatggcaccg cgtttaccgg
    26821 acctaaaata tcctcccctg aagttcggat aagcgaagcg catccgacaa ctcaccccgc
    26881 accgtgccca taaaaaaacc ccgccgaagc agggtctatg tgaactaaaa ttcgcttagt
    26941 gacgggcgcg caccagctga tacttacgtg tcagatactc caccggtacg ctccagatgt
    27001 gcaccagacg cgagaacggg aacagcagga ataacgtcat ccccaacacc aggtgcagac
    27061 ggaagataaa tgccacacca tcaaggtgtt gagaagcacc accgtggaag gtcactaccg
    27121 actgcgccca gccaaccagt ttcatcatct cgctaccgtc catatgctgg gctgagaacg
    27181 gaatggtcag caagcccagc gcgcactgga taacgagcag cgacaggatc aggatatccg
    27241 ctccggtagt ggttgcacgc acgcgtgggc tgaacagacg acgcttcagc aacagcacgc
    27301 cgccaatcag acacagcaca ccgctggcac caccagcaaa catcgccatt ttctgtttca
    27361 cttcaatcgg cagccaggct tcatacatcc agtgcggcgt cagcataccg aagaagtgac
    27421 caacaaaaat ccccagaata ccgatatgga acaggttcga cgccaggttc atccctttgc
    27481 gatccagcat ctggctggac gccgcgcgcc aggtgtactg cccgtagtca taacgcagcc
    27541 agctaccaat caggaagacc gccccggcta tgtacgggta gatatcaaag aagaacatat
    27601 tcaggaattg cattagtgct gtcctccggt ggtgatattc agatattgcg gcgcgacggc
    27661 accggcaaag cgacgctgat gagcagtgat tgctgaatca ccgcagcctt tttcagcaaa
    27721 gaatttaacc tgctcttctt cccagactgc atccagcgcc tgcggcgtat cgtcgcgcgc
    27781 ttcatcggca attttttccg ccactttgtc gctgtcgatg gcggtattcg ccagtttcag
    27841 cagcagatca aacagcacgg cataacggct ttcacgctgt tgcagacgcg cgctcagcaa
    27901 tgccagaatc ggcgcaatat ctttcaaacc ctccacggct tcgctttgcg gcagctgcgc
    27961 caggtactcc agatacagcg gcagatggtc gggcaattcg cggctgttta actgcaagcc
    28021 gtgctgctcg tactgcgcca gcagatccac catcgcctgg ccgcggtcac gggattcacc
    28081 gtgaacatgt tcaaatagca acagtgacgt ggcgcggcca cggtcgaaca gttcgctgta
    28141 ctgcgcctgg gcatcgagcg gatccatcgt cgttaaatcg cgcaggaaaa tgcccagcgc
    28201 atgggcatcc tcttttggca gatttttcga cgcggcaatc gcctcaaaca tctcttgttg
    28261 atgctgccat aaggcagcat ccggatattc aaggagacgc gatacaatca cgagttcgat
    28321 catgggtgcg gctccgtttt gctggttacg tcgatggcat cgatacggcg gctgttgaac
    28381 agattgaatt tggtatctga accgtggcag ccatcaccaa aggtaaagcc gcagccattt
    28441 ttctccggga aggcttcccg cgccagttca cgatgactgc tcggcaccac aaagcgatct
    28501 tcgtagttgg caatagccag ataacgatac atctcctgtg cctgggcttc ggtcagaccg
    28561 acctcttcca gcgcacgggt atcgacttta ccgtcaacgg tttcagcacg tttgtaatga
    28621 cgcatcgcca gcatacgttt cagcgcgcgc agtaccggtt tggtatcacc ggcggtcagc
    28681 agattcgcca gatactgtac cggaatacgc aggctttcga cgtctggcag aatgccgttg
    28741 ctgcccagtt cacccgcgtc tgccgcagac tgaatcggcg acagaggcgg cacgtaccag
    28801 accatcggca gtgtgcgata ttccggatgc agcggcagcg ctagtttcca ttccattgcc
    28861 attttataaa ccggcgactg ctgtgcggct tcaataacgc tcagcggaat accgtcttta
    28921 atcgcctgct cgatgacttt cggatcgttc ggatcgagga acacgtccag ctgacgctgg
    28981 taaagatctt tctcgttctc agtgctggct gcacgttcaa tagcgtcggc atcgtacaac
    29041 agcacgccga gataacggat acgaccgaca caggtttctg agcacacggt cggctgaccc
    29101 gcttcaatac gcggatagca gaagatgcac ttctcagact taccgctctt ccagttgaaa
    29161 tagatttttt tgtacgggca tccggtgatg cacatacgcc agccacggca tttatcctgg
    29221 tcgatcagga cgatgccatc ttcttcacgc ttgtaaatcg caccgctcgg gcaggtcgcc
    29281 acacatgccg ggttcaggca gtgttcgcac aggcgcggca aatacatcat gaaggtgttt
    29341 tcgaactggc tatacatcgc cttctggatg ttgtcgaagt tcttgtcttt cgccagcttg
    29401 tcgaactcac cgcccaggtc atcttcccag ttcggccctt tttcgatttt cgccatccgt
    29461 tcgccggtaa tcagcgaacg cggacgggca atcggctgcg acttgctgcc ttccggcgcg
    29521 gtatgcaggt tctgatagtc aaaatcgaac ggttcgtaat aatcgtcgat ccccggcaga
    29581 tgcgggttag cgaagatttt acccagcagc atggcgcggt tacccatgcg cggctgcagt
    29641 ttgccgttga ttttacggat ccagccgcct ttgtattttt cctggttttc ccagtcagtc
    29701 gggaagccct ggcccggctt ggtttccacg ttgttgaacc acgcgtattc cacgccttcg
    29761 cggctggtcc agacgttttt acaggtaact gaacaggtgt ggcagccgat gcacttatcg
    29821 agattcagca ccatgccgac ttgtgaacga attttcattt tacgctctcc tgtacctggt
    29881 cattgccttc gccatctaac cagtcaatgt tcttcatctt acgcactaca acaaactcat
    29941 cgcggttaga acctacggtg ccgtagtagt taaagccgta tgccagatgg gcatagccgc
    30001 cgatcatatg cgtcggtttc ggcgtgatac gggtgaccga gttatggata ccaccacgct
    30061 gttgggtaat ttccgaacca ggcaggttaa cgatacgttc ctgcgcgtgg tacatcatgg
    30121 tcatccctgc cggaacacgc tggctgacaa ccgcacgggc agtcagagca ccgttgctgt
    30181 tgaagacttc aatccagtcg ttatcagcaa tgcccagatc tttggcatcg gcttcactca
    30241 gccagaccac cggaccaccg cgacctaaag tcagcatcag caggttgtcg ctgtaggtgg
    30301 agtggatacc ccacttctgg tgcggcgtca ggaagttgag cgctttttcc gggttgccgt
    30361 tggatttctg ccccatcact tctttcaccg aacgggtgtc gatcggcgga cgataaacca
    30421 gcaggctttc accgaaatca cgcatccact ggtgatcctg atacagttgc tgacgaccag
    30481 agagcgtacg ccatgggatc agctcgtgaa cgttggtgta accggcgttg taagaaacgt
    30541 gttcatcttc cagaccagac caggtcgggc tggagataat tttgcgcggc tgtgcctgaa
    30601 tatcgcggaa gcggatcttc tcgtcttctt tattcagcgc cagatgcgta tggtcacgac
    30661 cggtaaattc gctcagggca gcccaggctt tcacggctac ctgaccgttg gtttccggtg
    30721 ccagtgtcag gatcatctct gccgcatcaa ttgcggtgtt cagcatcggc tggcctttcg
    30781 ccggaccttc tgctttggtg taattgagct tacgcagcag atccatctcg ctctgggtgt
    30841 tccaggcaat ccctttaccg ccgttaccga ttttctccat cagcgggccg atagaggtaa
    30901 agcgttcgta agttgccgga taatcgcgct ctacgaccat aatgtgcggt gcagttttgc
    30961 ctgggatcag gtcgcattcg ccttttttcc agtctttcac atccagcggc tgcgccagtt
    31021 cagcggcaga gtcgtgctgg ataggcagcg tgacgatgtc ggtttcttta cccagatggc
    31081 cgacgcacac ttcggagaat ttcttcgcga tggctttgta gatttcccag tcgcttttcg
    31141 cttcccaggc cggatcgacc gccgcagaca gcgggtgaat aaacggatgc atatccgaag
    31201 tattcatgtc gtctttttcg taccaggtcg ccgtcggcaa aatgatgtcg gaatagagac
    31261 aggtgctcga cagacggaag tccagcgtca ccaccagatc cagcttgcct tccagaccgt
    31321 tgtcctgcca gtccacttct tccggcttca cgccgccctg ctgccccagg tctttgccct
    31381 ggataccgtg ctccgtcccc agcaggtact tgagcataaa ctcatgacct ttaccggaag
    31441 aaccgagcag gttagaacgc cagatgaaca gattacgcgg gtggttttta ccgttttccg
    31501 gctgttccgc agcgaaacgg atagaaccct ctttcaggga tttcaccgta tagtccaccg
    31561 gattcatccc ggctttttct gcttcgcgag cgatagtcag cgggttagtg cctaactgcg
    31621 gcgcagacgg cagccagccc atgcgttccg cacggacgtt aaagtcgatc aggtgtccgg
    31681 tataacggga tttatccgcc atcggcgaca gcaactcgtc tgcggtcacg gtttcataac
    31741 gccactggct ggagtggtta tagaaataag aggtgctgtt catgtgacgc gccggacgct
    31801 gccagtcaag agcaaacgcc agcggctgcc agccggtttg cggacgcagt ttttcctgac
    31861 ctacatagtg cgcccagccc cccccgctct gaccgacaca gccgcagaaa atcagcatgt
    31921 tgatcagacc acgatagttc atatcgaggt gataccagtg gttcagtccc gcaccgacga
    31981 taatcatcga acgaccgtgc gttttatcag cgttatcggc aaattcacgg gcgatgcgaa
    32041 taatctggct gcgagaaacg ccggtaatct gctctgccca ggcaggggtg taagctttca
    32101 catcgtcata gctggttgca cagttaacgt cgttcaggcc acgttccaga ccatagtttg
    32161 ccagcgtcag atcataaacg gtggtcacca gggcggtgct gccatcagcc agttgcaagc
    32221 gtttcaccgg cagcttgtgc agcagcacgt tttccagttc cactttgttg aagtgttccg
    32281 tgccgtcgcc accaaagtac gggaagccca cctcagcgat ctcatcctgg ctacccagca
    32341 ggctcagttg cagctcggtt tcttcgccag ttttgccgtc gcgctgctca agattccatt
    32401 tgcccttctc gccccagcgg aagccaatag aaccgttcgg cgcaaccatt tcgccattgg
    32461 tattaaaggc gacagttttc cattccggat tgttttcctg gcccagcgca tccaccagat
    32521 cggcagcgcg cagcatacga cccgcagcgt agtaaccgtc gcgctcttcc agcatcacca
    32581 gcatcggcat gtcggtgtag cgacgcacat agtcggtgaa atactggctt gggttgtcga
    32641 ggtggaattc acgcagcatt acgtggccta tcgccagcgc cattgccgca tcggtgccct
    32701 gtttcggtgc cagccacaga tcgcacagtt tggcgatttc agcgtagtct ggtgtgacgg
    32761 caacagtttt ggttcctttg taacgcactt cagtaaagaa gtgagcatcc ggggtacgcg
    32821 tctgcggcac gtttgaaccc caggcgatga tgtagctgga gttgtaccag tcagcagatt
    32881 ccggtacgtc agtttgctca ccccaggttt gcggagacgc aggaggcagg tcgcagtacc
    32941 agtcgtagaa gcttaagcaa gtaccgccaa tcagcgagag atagcgcgca cccgatgcgt
    33001 aagaaaccat cgacattgcc ggaattggcg agaagccagc gacacggtcc ggaccgtaat
    33061 ttttgatggt gtaaacgtta gatgcggcga tcagttcgtt cacttcctgc caggaagaac
    33121 gaacaaaacc accgcgtcca cgcgcctgct taaagctttt cgctttatcg gcgtcttcaa
    33181 tgatagaagc ccatgcctca accggatcgc tatgcagcgc cttcgcttca cgccacattt
    33241 tcatcaggcg tttgcgcatc atcgggtatt tcaggcggtt agcgctgtaa agataccagg
    33301 agtagctggc accgcgcggg cagccgcgag gttcatggtt tggcaggtcc gggcgagttc
    33361 gcggatagtc agtctgctgg gtttcccagg tgaccagacc gtttttgacg tagattttcc
    33421 agctgcagga gccggtgcag tttaccccgt gggtagagcg gacgattttg tcatgctgcc
    33481 aacgctggcg atatccatcc tcccagtcac ggttggtatt gagaagctgg ccatgcccat
    33541 cggcaaaggt ttcacccttc tgtttgaagt agcgaaaccg gtccaggaat ttactcatcg
    33601 gttttctcct gtgggagcct gtcggccctc tgataaatcg acattgctaa tgtttaaggg
    33661 caaggtaacg ctctgaaaca gcgtgggaat tgataacgat caagagtgat ggggatgaaa
    33721 aataaagtaa attcctggaa taccccttta ggataattaa catttatagc ataacagact
    33781 gtaaattaag acgttttaca cctgtggtta tttaaaccat taagattttt atgcagatgg
    33841 gtattaagga gtattcccca tgggtaacat attaatgact cttaatgaaa tccagcgaaa
    33901 taagccatac aacattagta tgttgtaact aaatgacgca taaaaaaagc gcgacatcat
    33961 gccgcgcaaa ggataatcaa atattacgat ttatttttta gaatgccgac catataccgc
    34021 ccaggtaatc accacgcagg cgatatagaa aatcaaaaat actttcattg cgccgactgg
    34081 cgaacccgtt aatgccagcg agctaccaaa cgctttcggg ataaagaagc caccaatcgc
    34141 gccaatcgca gagatgaaac ccagcgccgc cgccgtgtcg gttgccgctt cacgcatcgc
    34201 acgttcgtca gaaccccctt ctgctttcac acgatccatt gtcagtttac ggaagatcac
    34261 ggaaatcatc tggaaagtgg aaccactacc cagcccagcg gtcaggaaca gcgccaggaa
    34321 gactgcgaag aacgccatga agcttccgcc ctgtccgtca gtcggtaagg tcaggaacag
    34381 caggccgctg aaaatcgcca tcaggataaa gttcaccagc gtgacacgag ttccgcccag
    34441 acggtcagat aatgcaccac ctgcagaacg cgccagcgca ccaataaacg gcccgaagaa
    34501 agcgtattgc agaatctgaa catccgggaa ctgcgttttt gacagcatcg caaagcccgc
    34561 ggagaagccg atgaaggagc cgaaggttgc cagatacagc aggctcataa tccacagatg
    34621 accccgtttg agtaccggca actgctcctt gatggaggct ttcgaggtag caagatcgtt
    34681 catgccaaac cacgccgcaa tggtgaagat ggcaaggaac ggcacccata tccaggacgc
    34741 attcgccaga tacagctcag tcccatccgg ctgtttgacg ccctggctac caaatactgc
    34801 gaaaatcgac agtgatacca ccagcggagc aaccaactgc atgacgctga cgcccatgtt
    34861 gcccagacca ccattcagac ccagcgcgcc accctgcttc tgtttcggaa agaagaagct
    34921 gatgtttgcc atactggatg cgaagttcgc gccagcaaag ccgcacagca gagaaatgat
    34981 gatgaagacg ctataaggcg tggaggtatc ctgcacggca aaacccagcc agacgcaagg
    35041 aataatcaga ataccggtgc tgaacgccgt ccagcgacga ccaccgaaga taggaaccat
    35101 aaaggagtat ggaacacgta ataacgcgcc agaaaccgaa ggcagcgcag tcaacataaa
    35161 tagctgatcg gtcgtaaaat taaaaccgac tttcggtaag ttcaccgcaa cagcgctgaa
    35221 caacatccat acgcaaaacg ccagcagcag acagggaacg gaaatccaca ggttgcggct
    35281 ggcaatacgt tgaccgcgtt gttgccagaa cgcaggatct tccggtcgcc aatctgtaat
    35341 gacagctcca gtagcccttt cgggggcgga tgagtgactc atagacacct ctgatactcg
    35401 tttcgatgtc tgccacctta gtgtctgtag ctaaaggcaa tttgatgtaa atcaaacgat
    35461 aagataactt tatctatcat tgatatttat cattacccat agtgagtaca gtgatttcat
    35521 aaaaattatg agatttttca cggtgctgta aaatccctac ccttaccgat gtaaagcgac
    35581 taaccacgcg gcaaataagg agtaactctt tccgggtatg ggtatacttc agccaatagc
    35641 cgagaatact gccattccag aatgtatcgt cacattcatt aaggttattg ctcatttaaa
    35701 gcctgaagga agaggtttac atgcttaaac gttgtctctc tccgctcacc ctggttaatc
    35761 aggttgcgct tattgtgttg ctttctactg ctattggact ggcagggatg gcagtttctg
    35821 gctggctggt gcaaggcgtt cagggcagcg cccatgcgat caacaaagcg ggatcgctgc
    35881 gcatgcaaag ttaccgtctg ttggcggcag tgccattaag cgagaaagac aagcccttaa
    35941 ttaaagaaat ggaacaaacg gcatttagcg ccgagttgac tcgagcagcg gaacgagacg
    36001 gacaactggc gcaattacag ggtttacaag attactggcg taatgaactg atccctgcgc
    36061 tgatgcgtgc acaaaaccgc gaaacggtgt cagcggatgt cagccagttt gttgccgggc
    36121 ttgatcagct ggtatctggt tttgatcgca ccacggaaat gcgcattgag acagtggtgc
    36181 tggtccatcg ggtaatggcg gtatttatgg cacttttact ggtattcact attatctggt
    36241 tgcgggcgag attgctacaa ccgtggcggc aactgctggc aatggcgagt gccgtcagcc
    36301 atcgcgattt tacccaacgc gcaaacatca gcgggcgcaa cgaaatggcg atgcttggaa
    36361 ctgcgttgaa caatatgtct gcagaactgg ccgaaagtta tgccgtactt gagcagcggg
    36421 ttcaggagaa aaccgccggg ctggagcata aaaatcagat cctctctttt ttatggcagg
    36481 ccaaccgccg attgcattcc cgcgccccgc tgtgtgaacg cctgtcacct gtactcaacg
    36541 gattacagaa tttaaccctg ctacgtgata tcgaactgcg ggtgtatgac actgatgatg
    36601 aagagaatca tcaggagttt acctgccagc cagatatgac ttgtgatgat aaaggctgcc
    36661 agctctgccc gcgcggcgta ttacccgttg gcgatcgcgg cacaaccctg aagtggcggc
    36721 tggctgacac tcatacgcag tacggtattt tgctggcgac cctgccgcag gggcgtcatc
    36781 ttagccatga tcaacaacaa ctggtggata ccctggttga acaactcacc gccacgctgg
    36841 cgctggatcg ccatcaggaa cgtcagcaac agttgatcgt gatggaagag cgtgccacca
    36901 ttgcgcgcga actgcatgat tctattgccc aatctctctc ttgcatgaag atgcaggtga
    36961 gttgcttaca gatgcagggc gatgcgctgc cagaaagcag ccgcgaactg ttaagtcaga
    37021 tccgtaacga actgaatgca tcctgggtgc agttgcgtga attgctcacc acattccgtt
    37081 tgcagctcac cgagcctgga ttacgtccgg cgctggaggc gagttgcgaa gagtacagcg
    37141 ccaaatttgg cttcccggtg aagctggatt atcaattgcc gcctcgtctg gtgccttcgc
    37201 atcaggcaat ccacttgttg caaattgccc gtgaggcatt aagtaacgcc ctcaaacatt
    37261 cgcaagcgag tgaggtcgtg gtgacggtgg cgcaaaacga taatcaggtc aaactgaccg
    37321 tccaggataa cggctgcggc gtgcctgaaa atgccatccg cagcaatcac tacggcatga
    37381 taataatgcg cgaccgtgcg caaagtttac gaggcgattg ccgcgtccgc cgtcgtgaat
    37441 caggtggcac cgaagtggtg gtcaccttta ttcccgaaaa aactttcaca gacgtccaag
    37501 gagataccca tgagtaatca ggaaccggct actatcctgc tgatcgacga tcacccgatg
    37561 ttgcgaactg gcgtaaaaca gcttatcagt atggcaccag atatcactgt ggttggcgaa
    37621 gcgagtaatg gcgaacaggg tattgaactg gcggagtctc ttgatcccga tctgatcctg
    37681 ttagatctca atatgcccgg catgaacggt ctggaaacgc tggataaact gcgcgaaaag
    37741 tccctctccg gtcgcattgt ggtattcagc gtctctaacc atgaagaaga tgtggtcacc
    37801 gcactgaaac gcggcgcgga tggctatctg ttaaaagata tggaaccgga agatctgctg
    37861 aaagcgttac atcaggcagc tgctggcgaa atggtattga gcgaagcatt aacgcctgtt
    37921 ctggccgcca gcttgcgcgc taaccgtgcc actactgagc gcgatgttaa ccagttaacc
    37981 ccacgcgagc gcgatattct caagctgatt gcccagggtt tgccgaacaa gatgattgcc
    38041 cgccgcctgg acatcaccga aagcacagta aaagttcacg tcaagcacat gctgaagaaa
    38101 atgaagctaa agtctcgcgt ggaagctgcg gtatgggtgc atcaggagcg cattttctga
    38161 ttacggttcc cagcgcagtt cgtcgtttga caatgcgtcg aacggttcga caagcgttag
    38221 cgtgatctca ttggaggaga cacgctgccc ctggttatct tccaccacca ctgacaatcg
    38281 ccagtgattg cttgcgcctt ccccgttttg ccagtcaggc atgatcagcg tccagccctc
    38341 ctcactgttg gcttgtgcgc ccggcgtcaa acttaatatc tgcgtatcgc cctgccaaat
    38401 cagttgccga ataccgtaac gactgcggat ttgtaatttc agcggcactg tttcgccagg
    38461 ttttagatcc cacggcggtg tcgccagaaa caccgttaac gtttttcgct gtcggtactc
    38521 aagagtcgga agattatttc gctgcggatt gtcatagcga ctaccacgta acgactgact
    38581 ttcgacaacc tcgcccgcag aaagttgttt tttgagcggt acaccaaagc ggtagttaag
    38641 attcagcccg aggttatttt gattctcgcc actttcaccc tgtttatgct gggccgtcac
    38701 agtgactaag ggcacagggg tgtaatttaa tcccagactc aacgcgacgg gattgtgata
    38761 acccgtacca gagttaaaca aatcgacacg atcaccaaaa tactgttcta cgctgacact
    38821 ggtattgaag tgttgataga acggcatgcg catccgggct gtcaggtcgt acccgcgcgc
    38881 catccgttgt tcctgcgtgg ctgtctgttc atgccatgca gcaaacggct gataaaagtt
    38941 tgccgatagt cgcaaatatt cgccccacgc ttcggcaccg aagcccgctc gctgaagatt
    39001 ttcgtccagc aagttgtcat aaaaagtgtt ataacccacc agccagctgc cgcgcgccca
    39061 gcgttgccca acgcccacat tgctcaccag cccatcatcc tgctgagtaa gaccaagctg
    39121 gctccaggtg agataacgat cattatcttg taacggcaca aaccagcttc cacgactgcc
    39181 ggtgaaatgt ccttcgttat ccactttgac gtcaacactg gcatttcccc acgctgatag
    39241 ccacgactct acgtgctgat taacctgttg actaaacgcg tcgcggactt ttcccaatgc
    39301 gaaagctttt gcctgctcgc cagtatccag cccgttatca ttcatactgg tttcgccgaa
    39361 atctttcaca atttcagcaa agtgtttttc cccatcatga ttttcaggtg ccatgcctaa
    39421 atccggcaga ccatcattgt tattatcaaa gggatttgcc gctttttgct cgaaggtaga
    39481 ttgtgcgtta gctgtaccgc ctgccgccag caagagtaaa taaaacggaa taatgcgagg
    39541 aacgaaacgg ctcaaaacgt cggtaacttc cggtaaaaaa tgaaaaacca tagccctaaa
    39601 gaataacgat tactggcaac ttttgtcgta ttttctcgcg ctttcaggaa aatccttaaa
    39661 aatgtgaccg attggtcaga cgctgcagaa gcagtaagat agaagatgag tgtctaattc
    39721 cctgaataca ggagtaaata atgcaaaaaa tcgtgatcgt tgccaatggc gcaccttacg
    39781 ggagcgaatc cttgtttaac agcttgcggc tggccattgc gttacgagag caggagagca
    39841 atctggatct gcgtctgttc ctgatgtctg atgcggtcac agccgggttg cgcgggcaaa
    39901 aaccagggga aggctacaac attcagcaaa tgctggagat ccttaccgct cagaatgtac
    39961 cggtgaaatt gtgcaaaacc tgtactgacg gacgcgggat tagcacactt cctctgattg
    40021 atggggtgga aatcggtacg cttgtggaac tggcgcaatg gacgctgtca gccgataaag
    40081 tgctcacatt ttaatgtttg agggggcgtc tgacgcccca ctatgaggga agtttactca
    40141 ggcgagtccc ggccgtaaca caccatccgg ataattctcc gccagcagtt tttttaccga
    40201 taccagcaaa tcattcagcc catcgtcctg cattcccagt ttgataagct cctgttccag
    40261 tgaaaacagg tattgtgcgt tggttcccag tggaccgctc gccgccgcaa tcaacggcgc
    40321 gatgacctga gcgcgagtgt cagattcata ttctggatgt cgcgggtcca taataaacac
    40381 aatggcgttt actgtgcgtc catcatcaag atcaagctga caccaggttg gcagataaca
    40441 gccggtaatc atctcgcgct tccacaacag ggttagttcc tgctccagcg tctcttctgg
    40501 cagtcgatag gcgacgcctg tggtgcgtcc gccctctttc agtgcaagca tccgtcccgg
    40561 ctggtgcgca gtcccgcgcc cggcggtcag gcgcaggcaa aacgcgcgat gccatccaac
    40621 cagtgtaccg gtgcacgact cggtaaactc cagtgccgga ttccacatca acgatccata
    40681 accgaagatc caccccggtc cctcatcagg tcgacaagcc agcgtcgccg ccagcgaagc
    40741 cgcccgttgc tctgctgacc ataagagtga ttcttcaatg gcaccaaatg ccgttttaca
    40801 atcggcattc atcaagaaat cacgcgttat caccttacac ctccgccact gcatacttcc
    40861 ctgcgacaac tttttggtgt cttccggaca tttttactgc tcattttgaa accactaacg
    40921 ggacaatatg caatctacgg accacttgca aggcaacacc gcacgaatag ctttaactaa
    40981 gctaccggtt ttacgatttt ttatgccatt tatcatcatc ccctttggca tattcatgct
    41041 tcacagcagc ccaggccact ttatgcgcgg tttcttcgcg actggcgtca tcacgccgat
    41101 cttctttatc tttatattga tcccatgcgc tgttgaacgc ttctttatag atatcctggg
    41161 catgagacgg tagaacgtgc tttacgcttt ccggcagatc gcttttcgtt ttatacggca
    41221 tcgttgactc cttttacaaa gttgaacatt aagtgtggtc aacaatatct actcttgcca
    41281 aaacataaga attttcatgt cagactcttc ttaaggagtg ctaaaacaaa cattgtctaa
    41341 acgcattgct tatttttatg gcccgatgag taaaaaatcg acagcggggt aaaaattcgt
    41401 aaaatgttac cgatatccag acactttctg ttatttttcc tacctatttt ttacattttc
    41461 gataaattca tctgcaaagg gataaccata atgtcaaatg ctcaagaggc ggtaaaaacc
    41521 cgccacaagg agacttcgct tattttcccg gttctggcgc tggtagtgct gttcctgtgg
    41581 ggaagcagcc agacactacc agtggtcatt ggcatcaatc ttcttgctct tattggtatt
    41641 ttaagtagcg cctttagtgt tgtccgtcat gcggacgtat tagcccatcg cctgggagaa
    41701 ccttacggtt cgcttattct tagcctttca gtggttattc ttgaagtcag tttgatttca
    41761 gctttaatgg caaccggcga cgccgcgcca acgctaatgc gtgatacgct ctattcaatc
    41821 attatgattg ttaccggtgg gctggttggc ttttcattat tgttgggcgg acgcaagttt
    41881 gccacccaat atatgaatct gtttggtatc aagcagtatt taattgcgct gttccccctg
    41941 gcgataatcg tactggtatt tccaatggct ctgcctgcgg cgaatttttc aaccggtcag
    42001 gcgttactgg tagcattaat ttctgcggca atgtatggcg tatttttgct gatccagacc
    42061 aaaacgcatc aaagtttgtt tgtctacgag cacgaagatg acagtgatga tgacgacccg
    42121 catcacggta aaccgtctgc ccatagcagc ctgtggcatg ctatctggtt gattatccat
    42181 ctgattgccg ttattgcggt gaccaaaatg aacgccagct cgctggagac attgctcgac
    42241 agcatgaatg cgcctgtcgc ctttactggc ttcctggtgg ctctgttgat tctgtcgccg
    42301 gaaggtttag gtgcgttaaa agcagtgttg aataaccagg tccagcgcgc gatgaatctg
    42361 ttctttggtt cagtgttagc aaccatttcg cttaccgtac ctgtcgtcac gttaattgcc
    42421 tttatgacgg gtaacgaatt gcagtttgca cttggtgcgc cagaaatggt ggtgatggtg
    42481 gcctctttag tactgtgcca tatctccttc tccaccggac gaactaacgt gctcaatggc
    42541 gcagcgcatc tggcactgtt tgccgcctat ttgatgacga tatttgcctg acagttatat
    42601 ctcctctgat gacagaaatc gggggagata atataaattc ctgcttattg tatttcttca
    42661 tactacctga acaggtcgtt aatcagaatt gcgagcctgt accacaacct gcacgaaacc
    42721 actgcaacag ctgttgaaaa gccggaatgt actctttatt gcaaacattt cagggatgaa
    42781 gtgaagatga attgagacgt aatatccaca ccaagccggc gttatccggt gaggcgcaat
    42841 gttgcggggg ctttatccct ggtggcattg gttgctggaa agagaaaacc cccgcacgtt
    42901 gagaggtaaa acctgacaac agaacggggg ctaatcttga accatcaaca attcaagaat
    42961 agccgcaaaa tgttgtcatt acaacaaggc ggctatatga cgctcgcgca gtttgccatg
    43021 attttctggc acgacctggc ggcaccgatc ctggcgggga ttattaccgc agtgattgtc
    43081 agctggtggc gtaaccggaa gtaaggtgaa aacgggcgtc aggctgccgt gatggcaata
    43141 tgcgcccgaa accggccgca ggggtaacag cctgcggccc tttttgtatc cggcccccat
    43201 gaaaaaagcc ttaacgcaga acgctaaggc tttagttttc aggcaaaaaa gattacttgc
    43261 tggtatccag ttcttcgaaa cctttcacca agtcatcaat cgctttcatc tgcttgagga
    43321 acggttccag tttagccagc ggcagcgcgg atggaccatc acatttcgca tgttccggat
    43381 ccggatgcgc ttcaataaac agccccgcca gacctaccgc cataccggca cgtgccaact
    43441 cagccacctg agcacgacga ccgccggaag ctgcgccaaa cggatcgcgg cattgcagtg
    43501 cgtgggtcac gtcgaaaatc accggcgaat taccagacac tttcttcatt atgctgaagc
    43561 ccagcatatc gacaaccagg ttgtcatagc cgaagttagc accgcgatcg caaagaatca
    43621 ctttttcgtt gccgccttct ttaaatttat caacgatatt acccatctgt cccgggctga
    43681 caaactgcgg tttcttgacg ttaattaccg caccggtttt cgccatggct tccaccaggt
    43741 cagtctggcg agcaagaaac gccggcaact gaatcacatc cacgacatcc gcaacgggct
    43801 gtgcctgact tggttcgtga acgtcggtga taattttcac accaaaagtc tgcttcaact
    43861 cctggaagat tttcatccct tcttccaggc ccggtccacg ataagagtgg atggaggagc
    43921 ggttggcttt gtcaaaagag gctttgaaca cgtaagggat acccagtttc tgggtcacgg
    43981 ttacgtagtg ctcgcaaatg cgcatcgcca gatcgcgaga ttccagcacg ttcataccgc
    44041 caaacagtac gaacggcagg tcatttgcta cgttgatgtc gccaatgcta accacttttt
    44101 gtttcatagg atcgccttaa tcttgagtaa aatgtcgatt aattaatgca gcacaatatg
    44161 tttatgcgcg atgttattta tttgcgcacg gatcatttcg ctgatcgggt cttccggaca
    44221 ctgttcaacg aaataactta aatcgttcaa cgcaacgtgt tcgcaatcca gttgcgcata
    44281 aatcaacccg cgatcgcgaa tttcataggg atcttccggg ttgaattgta ataaagcttc
    44341 gctggtgcgt aacgccagct ccatctgatt ttcttccatc aacgaagctt tgagtgtatc
    44401 cagcaatttg cggattacct caatgttatc agcttcatca aggtcttcat aaaacagttc
    44461 cgccgacggg ctgatatttc cctttaacca tacatccagc atatgttcgc ttaacgattc
    44521 accgttaaaa ggattaatca gccaaatttc gccatccgga cattcaatgc gcaatatcag
    44581 ctgcgtaggg aaaatcaccg gcagcagcgg caaatcgaga cgattcgcga cccataataa
    44641 aacggcgcct aacgataccg cactgccctg tcgattcttt aacacctggt ccagccataa
    44701 tgcatcggaa agacgataaa cgccgcgtga ggctttaaat ccccagtcgc cgtaaaacag
    44761 cgcgatcagt ttttccagct gctcttctaa aggcagaagc tggctgattt cttccttcgc
    44821 cagagtaacg agacgctcca gttcgtcgta aacatcttgc gagggaaaat cgcggcggat
    44881 tgcttcgcaa gccaggatca tgccttcgca caatggcgct ttattaaatt cgaaatcagc
    44941 taacgatctc atgacttacc ccagtaacgg tacttttgtg gtggcgagtt taatgatgat
    45001 gtacagcacc accaacgcca gcgggaaggc aataatgcgc gcctgctgac tgtgcatacg
    45061 acgataatcg agtgcaataa aacccaaaac gatataaatg ataactccaa acagcttttc
    45121 agtcagccac tgtgcctgcc cggaaaatgg caggatgtgc gctttagcca tcaacgcaat
    45181 gccgcttaac aataacaccg tatcgacaac cggcggcaca atgcgcgtcc aacgagcaaa
    45241 tgcctgtgga tgcttctgat aacgtagcca gaaacgtaag gtcaatagcc caacagaaag
    45301 cgcgatacta atgagatgaa cgctaagcag cgtagaaaaa cttgtcattg ataatagcgg
    45361 ccgagcgtta cgcgctcgtt atccccatag tcacggcagg tttcgacgtc atgatacccc
    45421 gcgaggataa atgcctgtcg caccgcttca ccctgctgcc agccatgctc cagaagcaga
    45481 aagccgccgg ataccagcgc gttacgcgac tgttcgatga tatgcacgat gtctgccatt
    45541 ccactgtctg ccgcaaccag cgcagtgagc ggctcaaagc ggacatcgcc ttgttgaaga
    45601 tgtgggtcct ggtcgtcaat atacggcgga ttgctgacaa tcatcgcaaa ctgctgcccg
    45661 gctagcgcgc taaaccagtc gctttgcaga atgtggatat ttttgatagc cagatgctgg
    45721 gcattacgtt gcgccagaga gacagcatct ggcatacgat ctacagcggt aatttcgcag
    45781 tccggacgct cgcaagccag cgccagcgca atcgccccgg tacccgtccc gagatcgaga
    45841 atacggcaag gttgttcagg caaccgcgcc agtgcctgct ccaccagaca ctccgtatcc
    45901 gggcgcggaa ttaaggtcgc tggcgaaaca aataacggca acgaccagaa ttctcgcacc
    45961 ccggttaaat gagcaatggg ttcaccatcg cgacgacgtg tcagtagcgc atcaagttgc
    46021 tgacattgtt cgtcagtcag ctgcgtttca ccaaaggcga gaataaaagt acgccctctg
    46081 ccggtaacat gctccagcag gatttcagca tcacgccgcg ggctttcgct cgcctgaagt
    46141 tggcttattg cttcacgtaa ccagtgttga tattccatta ttcctgctcg gacaacgccg
    46201 ccagttggtc ggcctgatgt tcctggataa tcggttcaat cagcatatcc agcttacctt
    46261 ccatcacttc atccaggcgg tagagcgtca ggttgatgcg gtgatcggta acgcgcccct
    46321 gcgggaagtt gtaagtacgg ttacggtcgc tgcgatcgcc actccccagc aggttacgac
    46381 gggtagacgc ttcggcctgt tgccgttttg ccatttcagc agcgtggatg cgagcaccga
    46441 gtacagaaag tgctttagct ttgtttttat gttgtgaacg ttcgtcctga cattcaacaa
    46501 caattccggt cggcaagtga gtaatacgaa ttgccgaatc ggtggtgtta acgtgctgac
    46561 cacccgcccc tgacgagcgg aaagtatcaa tgcgtaaatc tgctgggttg atgtccggca
    46621 gttctgcatc aggcagttct ggcattaccg caacggtaca agcagaagta tgaatacgac
    46681 cctgcgattc cgtagcagga acacgctgca cgcgatgacc gccagattcg aatttcaaac
    46741 gaccatatac accatcaccg ctaattttgg cgatgatctc tttataacca ccatgttcac
    46801 cctcgctggc gctcatgatt tctacacgcc agcggcgggc ttcggcgtaa cggctgtaca
    46861 tacggaacag atcgccagcg aacagcgccg cttcgtcgcc gccggttccg gcacggactt
    46921 cgaggaaggc gttacgttcg tcatcaggat cttttggtag taacagaacc tgtaattgct
    46981 gttccagttg ctcgcttttt tctttagctt cgcgcagttc atcctgcgcc atctcacgca
    47041 tttcaggatc gtcgagcatc atctgtgcgg tttcgatatc ttcctgaacc tgttgccagt
    47101 cggtaaaaca gcgcgaaaca tcacttaact gcgcatattc acgagataat gcgcgaaaac
    47161 gttcctggtc agcgatggtt tgcgcatcac ccagcagcgc ctgaacttct tcatggcgtt
    47221 catgcagggc ttccagtttg gcaacgatag aaggcttcat aggcgtaaat gcaccctgta
    47281 aaaaaagaaa atgatgtact gctactccag cccgaggctg tcgcgcagaa tattcaggcg
    47341 ttcgttatcc ccgtcacggg cggcctgttg aagtgatttt gttggtgcat ggatcaggcg
    47401 gttagtcagt ttccatgcca gatcctgcat aatggtttgc gcatcaccgc cctgttcaag
    47461 ggccgctaac gctttagcag ttaactcatc acgaacctgc tctgcctggc tacgataatc
    47521 gcgaatagtt tcgctggcgc tttgtgctcg aagccatgcc ataaattcac tggcctcctg
    47581 ggcgacgata gtttccgcct ccacagcagc ggctttccgt tgcgccaggt tgtgcgaaat
    47641 aatgctttgc agatcgtcaa cgctataaag ataagcattc gccagtttac caacttccgg
    47701 ctcaacatcg cgcggaacgg caatatccac cagcagcatt ggttgattgc gacggctttt
    47761 taatgcgcgc tccaccatgc ctttaccgat aatcggcaac gggctggcgg tggaactgat
    47821 gatgatatcg gcttcgcgca gacgttcgtc gatatcactc agggcaatca cttccgcgcc
    47881 aacctcatct gccagaattt gggcacgttc gcgagtgcgg ttggcgataa tcatcttctg
    47941 tactttgtgt tcgcgcagat gacgtgccac cagctcgatg gtttcgcccg cgccaaccag
    48001 cagtaccgtc accgtagaga gcgattcaaa gatctgccgc gccagcgtac aagccgcaaa
    48061 agcgacagac acagcactgg caccgatatc tgtttcagtg cgaacgcgtt tcgccacaga
    48121 gaaagatttc tgaaacatgc gttccagttc gctggccttc atatgacctt tttgcgaatc
    48181 ggcaaacgct tttttaacct gaccgaggat ctgcggctcg ccaaggacga gagaatcaag
    48241 cccactggca acgcgcatta aatgactgac cgcatcgtta tcctgatgcc agtagaggct
    48301 attacgcaga tcgtcttcgt tgagattatg gtaatcacac aaccagcgaa tgagcgcctc
    48361 ttgcaggtca tcccgttctt caacgctgag ataaagttct gtgcggttac acgtcgacag
    48421 caccacaccg ccctgcacca tcggctgcgc gagcagactg tcaagcgcct gatcgagctt
    48481 atccggcgaa aacgatacac gttctcgcag cgatacaggt gccgttttat ggttgatacc
    48541 gagtgctaaa agggtcatgt ctgcgggaaa taataccaac gttgataggg ttagtctgct
    48601 tgcatcatac aggatgcgta agatcaataa aagagagcgc ccccttttgg agtaattgcc
    48661 gaaagccgtt agattctggc aattaagaca acgtgaacat agacgatggc taacggtaac
    48721 gctagcatta agggttataa ctgcaacgta tctcaaggac ttgtcattat tatgcccctg
    48781 cccgattttc gtcttatccg cctgctaccg ctggctgctc ttgtgctcac tgcctgttcc
    48841 gttaccacgc ccaaaggtcc tggcaaaagc ccggattcgc cacaatggcg tcagcatcag
    48901 caagacgtgc gcaatcttaa tcagtatcaa actcgcggcg cgttcgctta tatttctgac
    48961 caacaaaaag tgtacgcccg tttcttctgg caacaaactg gccaggatcg ctaccgtctg
    49021 ctgctgacta acccattggg cagcacggaa ctggagctga atgctcaacc gggtaacgtg
    49081 cagttagtcg acaataaagg tcagcgttat acctccgatg acgccgaaga gatgattggc
    49141 aaattgaccg gaatgccaat tccgctcaac agcttgcgcc agtggatttt aggtttaccg
    49201 ggtgatgcga ctgactacaa acttgacgac cagtaccgcc tgagcgaaat cacctacagc
    49261 cagaatggca aaaactggaa agttgtttac ggtggttatg acacaaaaac tcaacctgcg
    49321 atgccagcca atatggaact caccgacggt ggtcaacgca tcaagttaaa aatggataac
    49381 tggatagtga aataatgcgg acacagtggc cctctccggc aaaacttaat ctgtttttat
    49441 acattaccgg tcagcgtgcg gatggttacc acacgctgca aacgctgttt cagtttcttg
    49501 attacggcga caccatcagc attgagcttc gtgacgatgg ggatattcgt ctgttaacgc
    49561 ccgttgaagg cgtggaacat gaagataacc tgatcgttcg cgcagcgagg ttgttgatga
    49621 aaactgcggc agacagcggg cgtctttcga cgggaagcgg tgcgaatatc agcattgaca
    49681 agcgtttgcc gatgggcggc ggtctcggcg gtggttcatc caatgccgcg acggttctgg
    49741 tggcgttaaa tcatctctgg caatgcggac taagcatgga tgagctggcg gaaatggggc
    49801 tgacgctggg cgcagatgtt cctgtctttg tccgtggtca tgccgccttt gccgaaggtg
    49861 ttggcgaaat attaacgccg gtagacccgc cagagaaatg gtatctggtc gcacaccctg
    49921 gcgtgagtat tccgacgccg gtgattttta aagatcctga actcccgcgc aatacgccaa
    49981 aaaggtcaat agaaacgttg ctaaaatgtg aattcagcaa tgattgcgag gttatcgcaa
    50041 gaaaacgttt tcgcgaggtt gatgcggtgc tttcctggct gttagaatac gccccgtcgc
    50101 gcctgactgg gacaggggcc tgtgtctttg ctgaatttga tacagagtct gaagcccgcc
    50161 aggtgctaga gcaagccccg gaatggctca atggctttgt ggcgaaaggc gttaatcttt
    50221 ccccattgca cagagccatg ctttaagccg ggcaagctga gtttcggtga caacgtcacc
    50281 ttgttccaga cgttgcatcg cgctctttaa tacaccgcct ggaaagaatc atgcctggcc
    50341 cgcacagttt tcggcagatt ctttccacca atggacgcat gcctgaggtt cttctcgtgc
    50401 ctgatatgaa gctttttgct ggtaacgcca ccccggaact agcacaacgt attgccaacc
    50461 gcctgtacac ttcactcggc gacgccgctg taggtcgctt tagcgacggc gaagtcagtg
    50521 tacaaattaa tgaaaatgta cgcggtggtg atattttcat catccagtcc acttgtgccc
    50581 ctactaacga caacctgatg gaattagtcg ttatggttga tgccctgcgt cgtgcttccg
    50641 caggtcgtat caccgctgtt atcccctact ttggctatgc gcgccaggac cgtcgcgtcc
    50701 gttccgctcg tgtaccaatc actgcgaaag tggttgcaga cttcctctcc agcgtcggtg
    50761 ttgaccgtgt gctgacagtg gatctgcacg ctgaacagat tcagggtttc ttcgacgttc
    50821 cggttgataa cgtatttggt agcccgatcc tgctggaaga catgctgcag ctgaatctgg
    50881 ataacccaat tgtggtttct ccggacatcg gcggcgttgt gcgtgcccgc gctatcgcta
    50941 agctgctgaa cgataccgat atggcaatca tcgacaaacg tcgtccgcgt gcgaacgttt
    51001 cccaggtgat gcatatcatc ggtgacgttg caggtcgtga ctgcgtactg gtcgatgata
    51061 tgatcgacac tggcggtacg ctgtgtaaag ctgctgaagc tctgaaagaa cgtggtgcta
    51121 aacgtgtatt tgcgtacgcg actcacccga tcttctctgg caacgcggcg aacaacctgc
    51181 gtaactctgt aattgatgaa gtcgttgtct gcgataccat tccgctgagc gatgaaatca
    51241 aatcactgcc gaacgtgcgt actctgaccc tgtcaggtat gctggccgaa gcgattcgtc
    51301 gtatcagcaa cgaagaatcg atctctgcca tgttcgaaca ctaatcgaac ccggctcaaa
    51361 gacccgctgc ggcgggtttt tttgtctgta atatccattt gtatgaccta tgcctccttc
    51421 acctgccatt tagttgacag atgatgcgct cacggatgaa acattattgt gaacaaaata
    51481 ttttcctcac atgtgatgcc tttccgcgct ctgatcgacg cttgctggaa agaaaaatat
    51541 actgccgcac ggtttacccg tgacctgatt gccgggataa ccgtcgggat tattgctatc
    51601 ccgctggcaa tggcgttagc tattggtagt ggtgtggcac cccagtacgg tttatatacc
    51661 gcagctgttg cggggattgt cattgctctg acgggtgggt cacgctttag cgtttccggt
    51721 ccgactgcgg catttgtggt aattctctat cccgtttcgc aacagtttgg actggcagga
    51781 ctgctggttg cgaccttgct gtcggggatc tttttgattc tgatggggct ggcacgcttt
    51841 ggtcgcctga ttgagtatat tccggtttcc gtcaccttag gtttcacctc gggtatcggg
    51901 atcaccatcg gtaccatgca gattaaagat tttctcggtc tgcaaatggc ccatgtcccg
    51961 gaacattatc tacaaaaagt cggcgcatta tttatggcgc tgccgaccat taatgtgggt
    52021 gatgcagcca ttggcattgt gacgcttggc attctggttt tctggccgcg tctgggcatt
    52081 cgtttgcctg gtcaccttcc ggccttgctg gctggttgcg cggtgatggg gattgttaac
    52141 ctgctcggcg gacatgttgc taccatcggt tcgcagttcc actacgttct ggccgatggt
    52201 tctcagggta acggtattcc gcaattgctg ccacaactgg tgctgccgtg ggatctacct
    52261 gattcagaat tcacgctaac ctgggattct attcgcaccc tgctgcctgc ggcattctca
    52321 atggcaatgc tcggcgcaat cgaatctctg ctctgcgccg tggtgctgga tggtatgacc
    52381 gggacgaaac acaaggcgaa cagcgaactg gttggacagg gactggggaa tattatcgct
    52441 ccgttctttg gtggtattac cgctacagct gccatcgcgc gttctgccgc taacgtccgt
    52501 gccggggcaa cttcccctat ctcggcggtg atccactcta ttctggttat tcttgccctg
    52561 ctggtactgg caccgctgct ctcctggctg ccgctttccg ccatggcagc cctgctgttg
    52621 atggtggcgt ggaacatgag tgaagcgcac aaagtggtcg acttgctgcg tcatgcaccg
    52681 aaagatgaca tcatcgtcat gctgctgtgc atgtcgctga ccgtgctgtt cgatatggtt
    52741 attgccatca gcgtggggat cgtgctggca tcgctgctgt ttatgcgtcg tatcgcacgt
    52801 atgactcgcc tggcaccggt agtcgtagac gttccagacg atgtcctggt tctgcgcgtt
    52861 attggcccgc tgttttttgc tgccgcagaa ggcttattca cggacctgga gtcacgtctt
    52921 gaaggcaaac ggattgtgat tctgaagtgg gatgccgttc cggtacttga tgctggtggt
    52981 cttgatgcgt tccagcgttt tgtgaagcgt ctgcccgaag gatgtgaact gcgcgtgtgc
    53041 aacctggaat tccagccact gcgcactatg gctcgcgcag gcattcaacc gatcctggga
    53101 cgcctcgcat tcttcccgaa tcgtcgcgcg gcgatggcgg atttataagt acgagattga
    53161 ccagtcagcg caaactgact ggtcagcaaa ctgcattatt tgttagctat gacggcgatt
    53221 gtcgctacgg cggcaacgtt tgtcatagtg gcgaacccac cagtaacggt cgcacacctg
    53281 ttcatgcccg ccaacacggg cacccgccag ccagagaatc gcaccgacga aaatacttag
    53341 cacagcacca tgtgcgaaat actggggcat attaaactgt ggtaactggt tgaggattga
    53401 ataccccacg ccgaccacca ttaccaccag acccaacccc atgagcacgt taccgagtaa
    53461 cgaagcgttt ttgcgtttca tatgtcacct ccggaacttt ctgggttgta acagggaata
    53521 cccctcttcc ttatgtgtaa agtatagaca acaccctgta ggttatgtgc gggtgtgatc
    53581 acaattacaa cccttatttc aacaaaactt tacaaataaa cgcctgaaca ccttgacttt
    53641 ctaatagtcc actcaggcaa taattgcatt atctgcattt gatacgcagt tttttgtcaa
    53701 gcggggccgc aaccagtaaa ctacgcgcca gttatgtaca cactcaggac aaaaaaacgt
    53761 gacgattaaa ttgattgtcg gcctggcgaa ccccggtgct gaatacgccg caacgcgaca
    53821 taatgctggt gcctggttcg ttgacttact ggcagagcga ttgcgcgctc cgctgcgcga
    53881 agaggctaaa ttctttggtt atacttcgcg agtcaccctt ggaggcgaag atgtccgcct
    53941 gttagtcccg actacattta tgaatctcag cggcaaagcc gttgcggcga tggccagttt
    54001 tttccgcatt aatccggacg aaattctggt ggcccacgac gaactggatc tgcctcctgg
    54061 cgtcgccaaa tttaagttgg gtggcggtca tggtggtcac aatggactga aagacatcat
    54121 cagtaaattg ggtaataacc ctaactttca ccgtttacgc atcggaatcg gtcatccggg
    54181 cgataaaaat aaagttgtcg gttttgtgtt aggcaaaccg cctgttagtg aacagaagtt
    54241 aattgatgaa gccatcgacg aagcggcgcg ttgtactgaa atgtggttta cagatggctt
    54301 gaccaaagca acgaaccgat tgcacgcctt taaagcgcaa taagtcgttg tctgcggcat
    54361 ttttgccgag tgccgtgtat aataggcaaa gttatttcca tttctgcaat ctgttagcaa
    54421 taacaggttg attattaaga tattaaggtg atttaaatca tgggattcaa atgcggtatc
    54481 gtcggtttgc ccaacgtcgg gaaatctacc ctgttcaacg cgctgaccaa agccggtatt
    54541 gaagcggcca actttccatt ctgcaccatc gagccgaaca cgggcgtcgt accaatgcct
    54601 gaccctcgcc tggatcaact ggctgaaatc gtaaaaccgc agcgtacact tcccacgacc
    54661 atggaatttg tcgatatcgc cggtctggta aaaggcgcat cgaaaggcga aggtctgggt
    54721 aaccagttcc tgaccaacat ccgtgaaacc gaagcgatcg gtcacgttgt tcgctgcttt
    54781 gaaaatgaca acatcattca cgtttccggc aaagttaacc cggcggatga tattgaagtt
    54841 atcaacaccg aactggcgct ggcggatctc gacacctgtg aacgtgcgat tcatcgcgta
    54901 cagaagaaag ccaaaggtgg cgataaagac gcgaaagccg agctggcggt actggaaaaa
    54961 tgcctgcccc agctggaaaa cgcaggtatg ctgcgtgcgc tggatttaag cgctgaagag
    55021 aaagcggctg ttcgttacct gagcttcctg acgctaaaac caacaatgta catcgccaac
    55081 gtcaacgaag acggttttga aaacaacccg tatcttgacc aggtgcgtga aatcgcagcg
    55141 aaagaaggtt ctgttgtagt tccggtttgt gctgctgttg aagcagacat tgccgaactg
    55201 gacgacgaag agcgtgacga gtttatgcag gagcttgggc tggaagagcc gggcctgaac
    55261 cgtgtgatcc gtgccggtta taagctgctg aacctgcaaa cttacttcac cgctggggtg
    55321 aaagaagtgc gtgcatggac cattccggtt ggtgcaaccg cgccgcaggc agcgggcaaa
    55381 atccatactg attttgaaaa aggctttatc cgtgcacaaa ccatctcgtt tgaagatttc
    55441 atcacttaca aaggtgaaca aggcgcgaaa gaagcaggca aaatgcgtgc agaaggcaaa
    55501 gattacatcg ttaaagatgg cgatgtgatg aacttcctgt tcaacgtcta attccgctaa
    55561 tactaccctg gcggttaata acgagccatt atgtgagttg cacgtaatgg cttttttacg
    55621 cctgctacgc gtcttctttc aattctgagt taccaaaact cataatcaga ctcctgacaa
    55681 tattaacaat gcagctttta tgtgataaca atcacagtgc attaaccgtt caagagtttg
    55741 tcatgaaaaa aacatttacc ttcagcctgt tagcaattgt ctttgcaagc ctgttgagtg
    55801 cttgtgttcc ccaccatcat caccgccaca gcgatggccc tcgtggtcct gcgccctccg
    55861 gtaagccaat gccaccttca aatggtgggc caaatcacgg cagtcatggt cgctattaat
    55921 tagcaattca tagcgggaaa gtacatactc tcccgctatc cacggcgctt aaactgccct
    55981 gcatcaatgc catgttgctt cattttccgc cacagcgtag tgcggccgat tccaagtaaa
    56041 gccgacattt cctgaatgcg accgcctgtg acctgggctg cgttaataat tgcctctttt
    56101 tcaacttccg caaatgacag actggtggaa aggcgagtgg cgctgacatc atctgtcgcc
    56161 tgctcggtaa agagatgttc aggtaaatca ctgacgcgaa tgcgcccgtt atcactactc
    56221 agagcaagat tctcgatgac gctgtaaagt tcaaaatcgt tgccaggcca tgcacaggaa
    56281 accaggcgag cgagggcatc gtcatcaatt ttcagccgcg tagagaagcg tttttcaaga
    56341 ctgtgtaatt tgttattcac cagcgccgga atgctgccac gccgcatacg cagaggcggg
    56401 atggtaattt caaatgcatg cagcgcgtaa tacagctggc gactaaaacg attttgttcc
    56461 accagcattg ccaggtccgc ggtcgttgtg gcaatcactt tgacatcaat gggtattaaa
    56521 cgccgcgcat ccagtcgcgt gataacaccc tgcttgataa cctgaagcaa agcagactgt
    56581 aactccaccg ccagatattc aatcttttca agaaacagcg tgccgccgtg cgccagttcc
    56641 agccgactca gacggccatt ttcattatcc gtgcgatcgc caccaataaa ttcttccgcc
    56701 agcgccgcat caccatataa ctcacaattg acggcgatat aaggccccgc tgcacgctcg
    56761 ctttcattat gaattgcctg acttagcagt gccttgccca cgccctcttc tccacaaagc
    56821 aggacaggaa agctactgcg cgccgcctgg cgaccaaaat gaatcaagcg gcgggtttgc
    56881 ggatcgtcct gtggcatatg agcgaaggta tggctgactt ttcctaattg actggtcatt
    56941 aactgccgca tctgttccac aggatggagc aacaaaataa agctggttcc ctgcgtttct
    57001 attatcggtt taagggttat caccgcatca ataaactggt gctggctttc aaaggttgct
    57061 tctacgtgtt tgagcggatg tgcctgtttt attgcttgtt gcaatacggc gggtaacgtt
    57121 aagagttcag tgattgcccg tccctgactt gccgtcgcgt caaggcgtaa gacccgcgcc
    57181 gcctgggcat taataaattg caaattaccc tgctcgtccc agctaatcac gccatcatcc
    57241 atactttcta acagggcatt aagttgattt aaatgacggt tagtttcagc gagcaaactg
    57301 tccgtcagca gtaaatttcc gacctcgcgt gcgattgcca acgtcaacgg caaatcagct
    57361 gcggtagttt gctcaaccgg acacgccagc gctattgttc ccgtcaatcg gcccttgctg
    57421 tcaaacaacg gcgttgcaca aaaggcccag ttccagagtg cctgtttgaa atgttgatcg
    57481 gccatcgttt tcacggcctg accagagata gccgctaacg atagcgcaca agtaccaata
    57541 attccctcgg cgcaatacgt gccgtcattg aatcccagtg cacttagttg ctgcaaggtt
    57601 tgcggatcgc cattacggct gagaatgcag gcggtttcat cgaggataag cagcgcacac
    57661 gctcgcggtg ccatatattc ccaggcatct tccagcgcag cctgcccgag cgtcagcatc
    57721 gctttcttac gccgataaat agacgcaaat gtcacgccct gggcctggtg tggtacgttc
    57781 catgtctccc gtttcatcag cttaatgcat cgctcccagg aggttgcaat taagggagat
    57841 atgtcccgac catcgttgtt aaaagcgcta ctcatatccc gcatccctta tgacaaaagg
    57901 tgttccggct cgcaatttta agtattaata cacgtaaaag tttcaatatg gaacattcat
    57961 taaagaatat gtttcactat gaaacataat ttgggctaat tttcttttcc cttgcctgat
    58021 gcacaatgga ttcatcggca gtcagtggtc gccgtgtcgt tgaacatcat ccatgcccta
    58081 ccgtaattgc tggagcaaaa taatgaaaaa attgatcaat gatgtgcaag acgtactgga
    58141 cgaacaactg gcaggactag cgaaggcgca tccatcgctg acactgcatc aggatccggt
    58201 gtatgtcacc cgagctgatg cccccgttgc agggaaagtc gccctgctgt cgggtggcgg
    58261 cagcggacac gagccgatgc actgtggcta tatcggtcag gggatgcttt cgggagcctg
    58321 tccgggcgaa attttcacct caccgacgcc cgataaaatc tttgaatgcg ccatgcaaat
    58381 tgatggcggc gaaggtgtac tgttgattat caaaaattac accggcgata ttcttaactt
    58441 cgaaacagca accgagttac tgcacgatag cggcgtaaaa gtgaccactg tggtcattga
    58501 tgacgacgtt gcagtaaaag acagtcttta taccgccggg cggcgcggcg ttgccaacac
    58561 cgtattaatt gaaaaactcg taggtgcagc ggcggagcgt ggcgactcac tggacgcctg
    58621 tgcggaactg gggcgtaagt tgaataatca aggccactca ataggtatcg ctctcggtgc
    58681 ctgtaccgtt cctgccgcgg gcaaaccttc ttttaccctg gcggataatg agatggagtt
    58741 tggcgtcggc attcatggtg agccaggtat tgaccgccgc cccttctctt cccttgatca
    58801 aaccgtcgat gaaatgttcg acaccctgct ggaaaatggc tcataccatc gcactttgcg
    58861 tttctgggat tatcaacaag gcagctggca ggaagaatca caaactaaac aaccgctcca
    58921 gtctggcgat cgggtgattg cgctggttaa caatcttggc gcaactccgc tttctgagct
    58981 gtacggcgtc tataaccgcc tgaccacacg ttgccagcaa gcgggattga ctatcgagcg
    59041 taatttaatt ggcgcgtact gcacctcact ggatatgacc ggtttctcaa tcaccttact
    59101 gaaagttgat gacgaaacgc tggcactctg ggacgccccg gtccacaccc cggcccttaa
    59161 ctggggtaaa taaggagata gcaatgtcac tgagcagaac tcaaattgtt aactggctca
    59221 ctcgttgtgg cgatattttc agcaccgaga gcgagtatct taccggactg gatcgcgaaa
    59281 ttggcgacgc tgaccacggg ctaaatatga accgaggctt tagcaaagtg gtggaaaaac
    59341 tccctgctat cgcagataaa gatatcggtt tcattctcaa gaataccggt atgacgctac
    59401 tttccagcgt cggtggtgcc agtggtccgc ttttcggtac cttctttatc cgcgccgcac
    59461 aggcgaccca ggcacggcaa agcctgacgc tggaagagct ttatcagatg ttccgcgatg
    59521 gcgcggacgg cgtaatcagt cgcgggaaag ccgaacctgg cgataaaacc atgtgtgatg
    59581 tgtgggtgcc ggtggtggaa tcgttacgtc agtccagcga gcaaaatctc tctattccgg
    59641 cggcgctcga agctgccagt agcgtcgccg aatccgctgc acaaagtacg attacgatgc
    59701 aagctcgcaa aggccgtgcc agttatctcg gtgaacgcag tattggtcac caggaccccg
    59761 gtgcgacctc ggtgatgttt atgatgcaaa tgttggcgtt agccgcaaaa gagtaaggaa
    59821 ttggtgatgg taaacctggt catagtttca catagcagcc gactgggaga aggcgtcggt
    59881 gaattagccc gtcagatgtt aatgagtgat aattgtaaaa tcgccattgc cgcgggaatt
    59941 gacgatccac aaaatcccat tggtaccgat gccgtcaaag tgatggaggc catcgaatct
    60001 gttgccgata ccgaccatgt gctggtcatg atggatatag gaagcgcatt attgagtgct
    60061 gaaactgcgc tggaattgct ggctcccgag atcgccgcaa aagtacgttt gtgtgctgcg
    60121 ccgttggtcg aaggtacact ggcagcaacg gttagcgcgg cctcgggggc ggagatcgac
    60181 aaagtgatct ttgacgccat gcatgcgctg gaagccaaac gtgaacaact gggtttaccc
    60241 tcctccaaca ctgaaatctc tgacacatgt ccttcgtacg atgaagaagc ccgttctctg
    60301 gcggtggtca taaaaaaccg taacggcctg catgtacgtc cggcctcccg gctggtttat
    60361 accttatcga catttaatgc cgatatgttg ctggagaaaa acggcaaatg cgtcacaccg
    60421 gagagtatta accagattgc gttactacaa gttcgctata acgatacgct gcgcctgatt
    60481 gcgaaagggc cagaagctga agaggcactg atcgctttcc gtcagctggc tgaagataac
    60541 tttggtgaaa cggaggaagt tgctccacct attctgcgtc ccgttccgcc tgtttcgggt
    60601 aaagcctttt attatcaacc ggttttatgt acggtacagg caaaatcaac cctgaccgtg
    60661 gatgaagaac aagaacgatt acgccaggcc attgacttca cgttattaga tctgatgacg
    60721 ttaacagcga aagcagaagc cagcgggctt gacgatattg ccgcaatctt ttctggtcac
    60781 catacactgt tagatgatcc ggaactgctg gcggcggcaa gcgaactcct tcagcatgaa
    60841 cattgcacgg cagaatatgc ctggcagcaa gttcttaaag aacttagcca gcaataccag
    60901 caactggatg atgaatatct acaggctcgc tatattgatg tggacgatct tctgcatcgc
    60961 accctggtcc acctgaccca aacgaaagaa gaactcccgc agtttaactc gccaacgatt
    61021 ctactggcgg agaacatcta tccctccaca atactgcaac tggatccggc ggttgtaaaa
    61081 ggtatttgcc ttagcgccgg aagtccgcta tcccacagcg ccctgatcgc ccgtgaactg
    61141 gggattggct ggatttgcca gcagggtgag aaactgtatg cgatacaacc agaagaaacg
    61201 ctaacgctgg acgttaaaac gcaacgtttc agccgtcagg gttaaaccac cgttcggatg
    61261 gcatcgttct gatgccatcc ggctatttac tctgcacgga tgatttttaa ctacctgtta
    61321 tattttttac caaatcatga tgattttttc tgcgctaatc gcgaacccaa cgccgctggc
    61381 tggctaacaa acagacgtta tttgatcact ctatttcgac gttatttatc cctttaattc
    61441 ccttttctaa aatgcctgac cgttcgcaga atgagatttc gatcatgcag ctagtgcgat
    61501 cctgaactaa ggttttctga tacttgaata ccgttttatt cggtttcgcc aaaggagaat
    61561 gattgatgaa atcccccgca gcttctcgcc cgctaaaaat ggcgttaatt ccagcctgca
    61621 tctttttgtg tttcgctgcg ctatcggtgc aggcagaaga aacaccggta acgccacagc
    61681 cgcctgatat tttattaggg ccgctgttta atgatgtgca aaacgccaaa ctttttccgg
    61741 accaaaaaac ctttgccgat gccgtgccga acagcgatcc gctgatgatc cttgctgatt
    61801 atcggatgca gcaaaaccag agcggatttg atctacgcca tttcgttaac gtcaatttca
    61861 ccctgccgaa agagggcgag aaatatgttc cgccagaggg gcaatcactg cgcgaacata
    61921 ttgacggact ttggccggta ttaacgcgtt ctaccgaaaa caccgaaaaa tgggattctc
    61981 tattaccgct gccaaaacct tatgtcgtgc caggcggacg ttttcgcgag gtatattact
    62041 gggacagtta cttcaccatg ttaggacttg ccgaaagcgg tcactgggat aaagtcgcgg
    62101 atatggtggc caattttgct catgaaatag acacttacgg tcatattccc aacggcaacc
    62161 gcagttacta tttaagccgc tcgcagccgc ccttctttgc cctgatggta gagttactgg
    62221 cgcagcatga aggcgacgcc gcgttgaaac aatacctgcc gcaaatgcaa aaagaatatg
    62281 cttactggat ggacggtgtt gaaaacctgc aagccggaca acaggaaaaa cgcgttgtaa
    62341 aacttcagga tggtaccctt ctcaaccgct actgggacga tcgcgatacg ccacgaccag
    62401 agtcatgggt ggaagatatt gccactgcca aaagcaatcc gaatcgacct gccactgaaa
    62461 tttaccgcga cctgcgctct gccgctgcgt ctggctggga tttcagctcg cgctggatgg
    62521 acaatccgca gcagttaaac accttacgca ccaccagcat cgtaccggtc gatctgaaca
    62581 gcttgatgtt caaaatggaa aaaatcctct cccgcgccag caaagctgcc ggagataacg
    62641 cgatggcaaa ccagtacgaa acgctggcga atgcccgtca aaaagggatc gaaaaatacc
    62701 tgtggaacga tcaacaaggc tggtatgccg attacgacct gaaaagtcat aaagtgcgca
    62761 atcagttaac cgcggccgcc ctgttcccgc tgtacgtcaa tgcggcagcg aaagatcgcg
    62821 ccagcaaaat ggcgacggcg acgaaaacac atctgctgca accaggcggc ctgaacacca
    62881 cgtcggtgaa aagtgggcaa caatgggatg cgccaaacgg ctgggcaccg ttgcagtggg
    62941 tcgcgacaga aggattacaa aactacgggc aaaaagaggt ggcgatggac attagctggc
    63001 acttcctgac caatgttcag cacacctatg accgggagaa aaagctggtg gaaaaatatg
    63061 atgtcagcac caccggaaca gggggcggcg gtggcgaata tccattacag gatggctttg
    63121 gctggaccaa tggcgtgacg ctgaaaatgc tggatttgat ctgcccgaaa gagcaaccgt
    63181 gtgacaatgt tccggcgacg cgtccactca gtgaatcaac aacacagccg ctaaaacaaa
    63241 aagaggcgga acctacgcct taactttact gtatatcgag caatgccatt acgtgcataa
    63301 aattggcatt gctctttctt catcacgaaa tacc
//
LOCUS       DAPHCV010000009        62025 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.009, whole genome shotgun sequence.
ACCESSION   DAPHCV010000009 DAPHCV010000000
VERSION     DAPHCV010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.009"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            228..2075
                     /gene="ldtD"
                     /locus_tag="RH033_000591"
     CDS             228..2075
                     /gene="ldtD"
                     /locus_tag="RH033_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDV2989826.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVVGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTIETAETKPMDKQTTSRSKPAPVVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2256..2804
                     /locus_tag="RH033_000592"
     CDS             2256..2804
                     /locus_tag="RH033_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HDV2989827.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2831..3478
                     /gene="gloC"
                     /locus_tag="RH033_000593"
     CDS             2831..3478
                     /gene="gloC"
                     /locus_tag="RH033_000593"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HDV2989828.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVALQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3529..4719)
                     /gene="aspC"
                     /locus_tag="RH033_000594"
     CDS             complement(3529..4719)
                     /gene="aspC"
                     /locus_tag="RH033_000594"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HDV2989829.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETIDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(4904..5992)
                     /gene="ompF"
                     /locus_tag="RH033_000595"
     CDS             complement(4904..5992)
                     /gene="ompF"
                     /locus_tag="RH033_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HDV2989830.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNISGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6594..7994)
                     /gene="asnS"
                     /locus_tag="RH033_000596"
     CDS             complement(6594..7994)
                     /gene="asnS"
                     /locus_tag="RH033_000596"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDV2989831.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8163..9365)
                     /gene="pncB"
                     /locus_tag="RH033_000597"
     CDS             complement(8163..9365)
                     /gene="pncB"
                     /locus_tag="RH033_000597"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDV2989832.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQYLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLADFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9631..12243
                     /gene="pepN"
                     /locus_tag="RH033_000598"
     CDS             9631..12243
                     /gene="pepN"
                     /locus_tag="RH033_000598"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HDV2989833.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDVPLRLNGEDLKLVSVYINDEPWTAWKEEEGALVISNLPERFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQLLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(12449..13216)
                     /gene="ssuB"
                     /locus_tag="RH033_000599"
     CDS             complement(12449..13216)
                     /gene="ssuB"
                     /locus_tag="RH033_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HDV2989834.1"
                     /translation="MNTARLNQGTPLLLNAVSKQYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGHSEQPIRRHG"
     gene            complement(13213..14004)
                     /gene="ssuC"
                     /locus_tag="RH033_000600"
     CDS             complement(13213..14004)
                     /gene="ssuC"
                     /locus_tag="RH033_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HDV2989835.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVMAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(14016..15161)
                     /gene="ssuD"
                     /locus_tag="RH033_000601"
     CDS             complement(14016..15161)
                     /gene="ssuD"
                     /locus_tag="RH033_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HDV2989836.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRNVAQS"
     gene            complement(15158..16117)
                     /gene="ssuA"
                     /locus_tag="RH033_000602"
     CDS             complement(15158..16117)
                     /gene="ssuA"
                     /locus_tag="RH033_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HDV2989837.1"
                     /translation="MRKIIKLALAGLLSVSTLAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQTKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLTTFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     LSAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(16110..16685)
                     /gene="ssuE"
                     /locus_tag="RH033_000603"
     CDS             complement(16110..16685)
                     /gene="ssuE"
                     /locus_tag="RH033_000603"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HDV2989838.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFVP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSTLKAQEILHGVFADDSQVIDYQHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            16922..17932
                     /gene="pyrD"
                     /locus_tag="RH033_000604"
     CDS             16922..17932
                     /gene="pyrD"
                     /locus_tag="RH033_000604"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HDV2989839.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSQELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            18106..18648
                     /gene="zapC"
                     /locus_tag="RH033_000605"
     CDS             18106..18648
                     /gene="zapC"
                     /locus_tag="RH033_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HDV2989840.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(18645..19754)
                     /gene="ycbX"
                     /locus_tag="RH033_000606"
     CDS             complement(18645..19754)
                     /gene="ycbX"
                     /locus_tag="RH033_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HDV2989841.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFAIQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            19998..22106
                     /gene="rlmKL"
                     /locus_tag="RH033_000607"
     CDS             19998..22106
                     /gene="rlmKL"
                     /locus_tag="RH033_000607"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HDV2989842.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            22118..24025
                     /locus_tag="RH033_000608"
     CDS             22118..24025
                     /locus_tag="RH033_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV2989843.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKIEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            24155..25408
                     /gene="pqiA"
                     /locus_tag="RH033_000609"
     CDS             24155..25408
                     /gene="pqiA"
                     /locus_tag="RH033_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HDV2989844.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            25413..27053
                     /gene="pqiB"
                     /locus_tag="RH033_000610"
     CDS             25413..27053
                     /gene="pqiB"
                     /locus_tag="RH033_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HDV2989845.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            27050..27613
                     /gene="pqiC"
                     /locus_tag="RH033_000611"
     CDS             27050..27613
                     /gene="pqiC"
                     /locus_tag="RH033_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HDV2989846.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            27869..28036
                     /gene="rmf"
                     /locus_tag="RH033_000612"
     CDS             27869..28036
                     /gene="rmf"
                     /locus_tag="RH033_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HDV2989847.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(28106..28624)
                     /gene="fabA"
                     /locus_tag="RH033_000613"
     CDS             complement(28106..28624)
                     /gene="fabA"
                     /locus_tag="RH033_000613"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HDV2989848.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(28693..30453)
                     /locus_tag="RH033_000614"
     CDS             complement(28693..30453)
                     /locus_tag="RH033_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HDV2989849.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARALQSDAGQLVGGHYEVSGHTIRLR
                     NAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTLTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANIR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            30639..31091
                     /gene="matP"
                     /locus_tag="RH033_000615"
     CDS             30639..31091
                     /gene="matP"
                     /locus_tag="RH033_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HDV2989850.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(31167..32207)
                     /gene="ompA"
                     /locus_tag="RH033_000616"
     CDS             complement(31167..32207)
                     /gene="ompA"
                     /locus_tag="RH033_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HDV2989851.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDVYTRLGGMVWRADTKSNFDGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFTFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(32564..33073)
                     /gene="sulA"
                     /locus_tag="RH033_000617"
     CDS             complement(32564..33073)
                     /gene="sulA"
                     /locus_tag="RH033_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HDV2989852.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQINQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            33292..33921
                     /gene="sxy"
                     /locus_tag="RH033_000618"
     CDS             33292..33921
                     /gene="sxy"
                     /locus_tag="RH033_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HDV2989853.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(33884..36046)
                     /gene="yccS"
                     /locus_tag="RH033_000619"
     CDS             complement(33884..36046)
                     /gene="yccS"
                     /locus_tag="RH033_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HDV2989854.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(36056..36502)
                     /locus_tag="RH033_000620"
     CDS             complement(36056..36502)
                     /locus_tag="RH033_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDV2989855.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFQ"
     gene            36625..38679
                     /gene="helD"
                     /locus_tag="RH033_000621"
     CDS             36625..38679
                     /gene="helD"
                     /locus_tag="RH033_000621"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HDV2989856.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSSEMSEIASGV
                     LQQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEVIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESHLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPSVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(38711..39169)
                     /gene="mgsA"
                     /locus_tag="RH033_000622"
     CDS             complement(38711..39169)
                     /gene="mgsA"
                     /locus_tag="RH033_000622"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDV2989857.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(39265..39927)
                     /locus_tag="RH033_000623"
     CDS             complement(39265..39927)
                     /locus_tag="RH033_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HDV2989858.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            40100..40513
                     /locus_tag="RH033_000624"
     CDS             40100..40513
                     /locus_tag="RH033_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDV2989859.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLAEVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(40558..40875)
                     /gene="hspQ"
                     /locus_tag="RH033_000625"
     CDS             complement(40558..40875)
                     /gene="hspQ"
                     /locus_tag="RH033_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HDV2989860.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(40933..42123)
                     /gene="rlmI"
                     /locus_tag="RH033_000626"
     CDS             complement(40933..42123)
                     /gene="rlmI"
                     /locus_tag="RH033_000626"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HDV2989861.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPNESIDIAFFTRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGPITGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            42218..42496
                     /gene="yccX"
                     /locus_tag="RH033_000627"
     CDS             42218..42496
                     /gene="yccX"
                     /locus_tag="RH033_000627"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDV2989862.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKKLGLSGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(42493..42822)
                     /gene="tusE"
                     /locus_tag="RH033_000628"
     CDS             complement(42493..42822)
                     /gene="tusE"
                     /locus_tag="RH033_000628"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HDV2989863.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGIALSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(42914..43573)
                     /gene="yccA"
                     /locus_tag="RH033_000629"
     CDS             complement(42914..43573)
                     /gene="yccA"
                     /locus_tag="RH033_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HDV2989864.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(43780..43867)
                     /locus_tag="RH033_000630"
     tRNA            complement(43780..43867)
                     /locus_tag="RH033_000630"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43831..43833),aa:Ser,seq:tga)
     gene            44294..45412
                     /gene="hyaA"
                     /locus_tag="RH033_000631"
     CDS             44294..45412
                     /gene="hyaA"
                     /locus_tag="RH033_000631"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HDV2989865.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            45409..47202
                     /gene="hyaB"
                     /locus_tag="RH033_000632"
     CDS             45409..47202
                     /gene="hyaB"
                     /locus_tag="RH033_000632"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HDV2989866.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFKNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            47221..47928
                     /gene="hyaC"
                     /locus_tag="RH033_000633"
     CDS             47221..47928
                     /gene="hyaC"
                     /locus_tag="RH033_000633"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HDV2989867.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEVRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            47925..48512
                     /locus_tag="RH033_000634"
     CDS             47925..48512
                     /locus_tag="RH033_000634"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HDV2989868.1"
                     /translation="MSEQLVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMRLEEQG"
     gene            48509..48907
                     /gene="hyaE"
                     /locus_tag="RH033_000635"
     CDS             48509..48907
                     /gene="hyaE"
                     /locus_tag="RH033_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HDV2989869.1"
                     /translation="MSNDTPFNALWQRMLARGWTPVSECRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGS
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            48904..49761
                     /locus_tag="RH033_000636"
     CDS             48904..49761
                     /locus_tag="RH033_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HDV2989870.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQQQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DGSESEIQEAIFCGLWRVRRRRGDKLLEDKLEAGCAPLALWQAATQNVLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEGAPS"
     gene            49895..51439
                     /gene="appC"
                     /locus_tag="RH033_000637"
     CDS             49895..51439
                     /gene="appC"
                     /locus_tag="RH033_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HDV2989871.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            51451..52587
                     /gene="appB"
                     /locus_tag="RH033_000638"
     CDS             51451..52587
                     /gene="appB"
                     /locus_tag="RH033_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDV2989872.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            52600..52692
                     /gene="cbdX"
                     /locus_tag="RH033_000639"
     CDS             52600..52692
                     /gene="cbdX"
                     /locus_tag="RH033_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HDV2989873.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            52772..54070
                     /gene="appA"
                     /locus_tag="RH033_000640"
     CDS             52772..54070
                     /gene="appA"
                     /locus_tag="RH033_000640"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HDV2989874.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELEQVLNFPQSNLCLNREKQDKSCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPAQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(54191..56371)
                     /gene="etk"
                     /locus_tag="RH033_000641"
     CDS             complement(54191..56371)
                     /gene="etk"
                     /locus_tag="RH033_000641"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HDV2989875.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIVSVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(56391..56837)
                     /gene="etp"
                     /locus_tag="RH033_000642"
                     /gene_synonym="yccY"
     CDS             complement(56391..56837)
                     /gene="etp"
                     /locus_tag="RH033_000642"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HDV2989876.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(56825..57964)
                     /locus_tag="RH033_000643"
     CDS             complement(56825..57964)
                     /locus_tag="RH033_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HDV2989877.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(58010..60106)
                     /locus_tag="RH033_000644"
     CDS             complement(58010..60106)
                     /locus_tag="RH033_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HDV2989878.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEPLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(60106..60852)
                     /locus_tag="RH033_000645"
     CDS             complement(60106..60852)
                     /locus_tag="RH033_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HDV2989879.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVVATIKS
                     VRQQLLNLKITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGTG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(60849..61493)
                     /locus_tag="RH033_000646"
     CDS             complement(60849..61493)
                     /locus_tag="RH033_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HDV2989880.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRDV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(61600..61905)
                     /locus_tag="RH033_000647"
     CDS             complement(61600..61905)
                     /locus_tag="RH033_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2989881.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
BASE COUNT        15255 a        15634 c        16242 g        14894 t
ORIGIN      
        1 tccgaaaact ccgtttctgc actaaaaaag tggcacatta cggcgcgctt ttctttaaac
       61 ttctttacat taggttatgt aaaaacgtat cggcgtttat atactgaaga taagcctgat
      121 gagtaacagg cttgctcgtc atactttcgt gagtattggc gttgtacagg caagtcgtaa
      181 aataacagcc tggctattca gagtatgata aaaacagggg gcaagggatg ttgcttaata
      241 tgatgtgtgg tcgtcggctg tcggcaatca gtttgtgcct ggccgtaaca ttcgctccac
      301 tgttcaatgc gcaggccgat gagcctgaag taatccctgg cgacagcccg gtggctgtca
      361 gtgaacaggg cgaggcactg ccgcaggcgc aagccacggc aataatggcg gggatccagc
      421 cattgcctga aggtgcggct gaaaaagccc gcacgcaaat cgaatctcaa ttacccgcag
      481 gttataagcc ggtttatctt aaccagcttc aattgttgta tgccgcacgc gatatgcaac
      541 ccatgtggga aaaccgtgat gctgttaaag ccttccagca acagctggca gaggtggcga
      601 ttgccggttt ccagccgcag tttaataaat gggtagagtt actgaccgat cctggtgtta
      661 acgggatggc acgcgacgtg gtgctctctg atgcgatgat gggctatctc catttcattg
      721 caaatattcc ggtcaaaggc actcgctggc tatatagcag taaaccttat gcgctttcaa
      781 cgccgccgct ttcggtgatt aaccaatggc agctggcgct ggataaaggt caattgccta
      841 cgtttgttgt aggattggca ccgcagcatc cgcaatatgc ggcgatgcat gaatcgttac
      901 tggccttact cagtgacacc aaaccgtggc cccaactgac cggcaaagca acgttgcgcc
      961 caggacagtg gagtaacgac gtaccggcgt tgcgcgaaat attgcaacgt acaggcatgt
     1021 tggacggggg accgaaaatt acgctacctg gcgatgacac gccaactgac gcggtagtca
     1081 gcccatccgc tgttactatt gaaacagcag aaactaagcc gatggacaag caaactacgt
     1141 ctcgtagtaa acctgcgcct gtcgttcgtg ccgcctacga taatgaactg gtggaagccg
     1201 ttaaacgttt tcaggcatgg caaggattgg gggcagatgg tgctattggc ccggcaacgc
     1261 gtgactggtt aaacgtaacg cccgcccagc gtgctggcgt gttggcactc aacatccagc
     1321 gattgcgctt gctgccaaca gagctttcta ccgggatcat ggttaacatt ccagcctatt
     1381 cgctggtcta ctatcagaac ggcaatcagg tgctggattc gcgagtcatt gtcggtcgcc
     1441 ccgatcgcaa aacgccgatg atgagcagtg cacttaacaa cgtagtggta aacccgccgt
     1501 ggaacgtacc accaactctg gcacgcaaag atattctgcc gaaagtgcgc aacgatccgg
     1561 gatatctcga aagccatggc tatacggtga tgcgcggctg gaacagcaga gaagcgattg
     1621 acccatggca ggttgactgg tctacaatca cggcctcgaa tttaccgttc cgcttccagc
     1681 aggctccagg cccacggaac tcgctggggc gctataaatt caatatgccg agttcagagg
     1741 ccatttattt gcatgacacg ccgaaccaca atctgttcaa gcgtgataca cgcgcattga
     1801 gctcaggctg tgtacgagtg aataaagctt ccgatctggc gaatatgctg ttgcaggatg
     1861 caggctggaa tgacaaacgt atttctgatg cgctgaagca gggcgatacg cgttacgtca
     1921 atattcggca gtcgattccg gtgaatctct actatctgac ggcctttgtt ggtgcagatg
     1981 gtcgtaccca gtatcgtaca gatatttaca attatgatct gcctgcgcga tccagctcgc
     2041 aaattgtatc gaaagcggaa caattaatca ggtaaatgaa gcagttcggg gaaatcagtt
     2101 gtcgtaataa ttagcatgat tgggggcaat tctctgcagc ccccgtcact gctggggttg
     2161 agtcatcttg acgtctgctt tacgggcggt taaggtgcct cttgtgcgcc agaagtgcat
     2221 ataaacgata acattgacct gtagacttga ttatcatgga caaattcgac gctaatcgcc
     2281 gcaaattgct ggcgcttggt ggcgttgcac tcggtgccgc catcctgccg acccctgcgt
     2341 ttgcaacact ctctacccca cgcccgcgca ttttgacact caataatctt cataccggag
     2401 agtcaatcaa agcggagttt ttcgatggca gaggctatat tcaggaagaa ttggcaaaac
     2461 ttaaccattt tttccgcgat taccgcgcga acaaaataaa gtccatcgac ccaggattat
     2521 tcgaccagtt gtatcgcctg caagggttgt taggtacccg caaaccggtg caactcattt
     2581 ccggttatcg ttccattgat accaacaatg aactacgcgc ccgcagccgt ggagtagcga
     2641 agaaaagcta tcacacgaaa gggcaggcga tggatttcca tattgaaggt atcgcgttaa
     2701 gcaatattcg caaagccgcg ttatctatgc gcgcaggtgg tgtaggatat tacccacgta
     2761 gtaactttgt gcatattgat accgggccag cacggcactg gtagcaattg cttaacgaaa
     2821 caggggcagt atgaactatc gtattattcc ggtcaccgca ttctcccaga actgttcatt
     2881 gatctggtgt gaacaaaccc gcctggccgc actggtcgat cctggcggcg atgcggaaaa
     2941 aatcaaacag gaagttgacg ccagcggcct gacactgatg cagatcctgc tgacgcatgg
     3001 tcatctggac cacgttggtg cagcggcgga actggcgcaa cattacggcg tgccggtttt
     3061 tggcccggaa aaagaagatg agttctggct gcaaggcttg cctgcgcaaa gtcgtatgtt
     3121 tggtctggaa gagtgccagc cgctgacgcc ggatcgttgg ctgaacgaag gcgataccat
     3181 cagcataggg aatgtggctt tacaggtgtt acattgccct gggcatacgc cgggtcatgt
     3241 cgtgtttttt gatgatcggg caaagctgct gatttctggc gacgttattt tcaaaggcgg
     3301 agtagggcgc agtgacttcc cgcgtggcga tcataatcaa ctgatttctt caatcaaaga
     3361 taagttgctg ccactggggg atgacgtgac atttattccg ggtcacggac cattatccac
     3421 acttggttat gagcgcctgc ataatccctt cctgcaagac gaaatgcccg tctggtaaag
     3481 gcgcataaaa aagcccgctt tttaagcggg cttcattgtt tttaacgctt acagcactgc
     3541 cacaatcgct tcgcacagcg gagccatatt atctggtgtc atcccggcca cgtttacgcg
     3601 accagaagca acagcatata cgccaaactc ttcgcgcaga cgcagcactt gttcttttgt
     3661 caggccactg aaggagaaca tgccgttctg tttgatgata aagctgaagt cgcgatttgc
     3721 gcctttttcc tgcagcgtat tgacgaacaa ctgacgcata cgctgaatac gctggcgcat
     3781 atcagtcagt tcttgttccc aaatcgcacg taacgcatcg ttgctcagga tggtggcaac
     3841 aacagaagcg ccgtgtgctg gtgggttaga gtagttagcg cgaatcgccg ctttcatttg
     3901 gctgaatgcg cgatcaatgg tttcactgtc agcagcaacc agagtacaag cgccaacacg
     3961 ctcgttgtac aggccaaagt ttttagagta ggaactggca acaatcagct ctttatgcat
     4021 agccgcgaaa gcgcgcagtc cttcagcatc ttcttccaga ccacgggcaa acccctggta
     4081 agcgaagtca aacagcggta accagccttt ctcaacggaa agttgtgcca gtgtctgcca
     4141 ttgttccagc gtagggtcga taccggttgg gttatggcag cagccatgga acagcaccac
     4201 gtcgccagcc tgagcttcgt tcaggctgtt aatcagtgca tcgaagtcaa gggtgtgatt
     4261 ttccgcatca taataagcgt attcacgaac ttccagacct gcagagttaa agacgctctt
     4321 atggttcggc cagcttgggt tgctcaccca cacacgctta acgctggtat tttttgccag
     4381 gaaatcggca gccacgcgta gtgcgccagt gccacccgga gtctgtgccg tgcgagcacg
     4441 tttgtcattg atcagggcgc tacctttacc aaacagcagt tcctgagtgc agcgaccaaa
     4501 ttcagggatg ccgtcaatgc cgaggtaatt tttggtggtt tcattttcga gcagatactg
     4561 ttcagccttt ttcacactgg tcagtaccgg ggttttgccc gtctcatctt tatagacacc
     4621 aatcccgagg ttaattttgc cgggacgttc atcggcacga aacagatcgg ccaggcccag
     4681 aatcgggtcg gcaggagcgg cggtaatgtt ctcaaacatg acgaggttcc attatggtta
     4741 cagaagggaa gtccgctatc agggtaacgg gagatttaca aaattccaac tattactgat
     4801 gaaaacgcag gctgtttttg caagacgtga gattgctctg gaaggcataa aaaaaacagg
     4861 accaaagtcc tgttttttcg gcatttaaca aagaggtgtg ctattagaac tggtaaacga
     4921 tacccacagc aacggtgtcg tcagaaccta cgcccagttt gttgtcagaa tcgatctggt
     4981 tgatgatgta gtcaacatag gtggacatgt ttttgttgaa gtagtaggtt gcgcccactt
     5041 caaagtagtt aaccagatca acatcaccga taccttctac gtctttcgct ttagatttgg
     5101 tgtaagcgat ggacggacgc agaccgaaat cgaactggta ttgcgcaact aacagaacgt
     5161 cttgcgtttt gttggcgaag ccgctgatgt ttgtaaattt attagtgatc ggcgtagcgt
     5221 tacgggtttc accgtagttc gctgccaggt agatgttgtt cgcgtcatac ttcagaccag
     5281 tagcccactg ttcagctttt ttaccgttgc caagaggttg agcttcttgc aggttggtac
     5341 ggtcagcggc gccataagca ccaacgatac caaagccttc gtattcgtag ctgatagaac
     5401 cgccaacacc gtcgccgtta gagcggcgtg cagtgtcacg ctcgttttta cccaggtact
     5461 gaacagcgaa gttcaggcca tcaaccagac caaagaagtt ggagttacga taggtagcaa
     5521 cgccgccaac acgaccaacg aagaagtcat cgctgtatgc cgtgtcaccg ccaaactcag
     5581 gcagcatatc ggtgtaaccc agtgcatcat aaaccacacc gtagttacgg ccgtaatcga
     5641 aagaaccaac gtcagcgtat ttaagacccg cgaatgccag acgcgttttg ttaccagttt
     5701 gagcgtcagc gccttcagag ttgttaccct ggaagttata ttcccactga ccataaccgg
     5761 tcagatcgga attgatttga gtttcccctt taaaaccaag acgggcatag gtcatgtcac
     5821 cattgccacc gtaactgttt tcaccgttac ccttggagaa ataatgcaga ccgacagctt
     5881 taccgtacag atctactttg ttgccatctt tgttatagat ttctgcagcg tttgcagtac
     5941 ctgctactaa cagagcaggg acgatcactg ccagaatatt gcgcttcatc attatttatt
     6001 accctcatgg tttttttatg acacctgcca ctgccgtcaa taagttctgt caataaaaat
     6061 ttacggaact attgatgaga gtttggtgtc tttatgtgtc tgcaggcatc tttccattca
     6121 aactaacgtt tcgctaccgt gaaagtgcta caaagataaa gatttggttt caaaaagaaa
     6181 aaatatgtaa ccaaaagtaa aatttaagga actttgtgaa catcgtcata tttccatata
     6241 ggcgtgatga tatttacagc aatttttatc tatttatatg atttccttat atttaaatta
     6301 actaaacgga aattttgttt ctgatggaaa ctttatcgac ctggcacaaa atcctctttc
     6361 agacatccag aatgccaaaa aatagtatga aaattgtgct atcagctaag aaaaaactaa
     6421 tccgcaataa gaatgcgggt tattttttgt gcgtaaatgt tgcaatgtaa tattccgtaa
     6481 caggatgatc gttatggcgg ggttgcaaat agattgcttg acttaacaaa cagataactt
     6541 gacagaaagg ataaaaaaag agccagcggg tgctggctct tgaagacgct aaattagaag
     6601 ctggcgttac gcggagtacg tgggaacgga atcacatcac gcacgttttg cacaccagtt
     6661 acgtaagcaa tcaggcgttc aaaaccaaga ccgaaacctg aatgcggaac agtaccgtag
     6721 cgacgcagat cgcgatacca ccagtaatct tctttattca ggcccatttc cagcatacgc
     6781 tcgtccagca cgtccagacg ttcttcacgc tgggagccac caatgatctc accgatgccc
     6841 ggagccagaa cgtccatagc cgcaacggtt ttaccgtctt cgttaaggcg catatagaac
     6901 gctttaatat ctttcggata gtttttaacc actaccggtg ctttaaagtg ttcttccgcc
     6961 agataacgct catgctcaga agagagatcg acgccccagt aaaccgggtt ttcaaacttc
     7021 ctgccgcagt tttcgagaat ggtcactgcg tcggtgtaat ccacctgcgc gaaatcggcc
     7081 tcaatgaagc gttccagacg tgaaacggca tctttatcta cgcgttcagc gaagaatttc
     7141 atgtcgtcag cgcgttcttc gagaaccgct ttgaagacat atttcagcat ggcttcagcc
     7201 agacccgcga tatcgttcag gttagcaaac gccacttccg gctccagcat ccagaattcc
     7261 gccaggtgac ggctggtgtt ggagttttca gcacggaaag tcgggccgaa ggtataaatt
     7321 ttggacaatg cgcaagcgta ggtttcgccg ttcaactggc cagatacggt caggaaagac
     7381 tctttaccaa agaagtcttt gtcgaaatcc actttgccct gatcgttacg cggcaggttt
     7441 tccagatcca gcgtagaaac acggaacatt tcgcctgcac cttcggtatc agatgcggta
     7501 atcagtggcg ttgaaaccca gaaaaatccc tgctcgttaa agaagcgatg cagcgcctgc
     7561 gccagcgtat ggcgaacgcg cgcgacggca ccaatcaggt ttgtgcgcgg acgcaggtga
     7621 gcgacttcac gcagatactc aatgctgtgg cgttttgccg ccatcgggta agtgtctgga
     7681 tcttcaaccc aaccagcaac ttcaaccttg ctggcctgaa tttcaaattg ttgcccctgg
     7741 cccggcgacg ccacgacttt acccgtcaca atgaccgagc agccggtggt cagacgcaag
     7801 acgtcttcat tgtaattggg cagagaatta ttgatgacag cctgtacagg atcaaagcag
     7861 gaaccgtcat aaacggcgag gaaggagatg ccagcttttg aatctcggcg ggtacgtacc
     7921 catccgcgca cggtgacttc gctgtcaacg gctacgcggc cctggagtac gtcggctaca
     7981 ggcacaacgc tcataatatt ctctctgtta atagtcggaa aaaataaaca cttgtccacc
     8041 cgtaaatggg gggataccta tgttacctgg catctgcaat cagacaagca gaattcgcaa
     8101 atgcagcgaa agatttcgga attaaaggta aaaaaaggga gccgattagc tccctgatga
     8161 tattaactgg cttttttaat atgcggaagg tcgaacgctt tgcgtagcgc ccgaacaaac
     8221 gctttatcgt ggcagatagt tttgcctggg ctgtcagaaa gtttcgccac tggtttaccg
     8281 ttacactcta ccaacttaat gacaatattc aggggtttta cctgggggat atcgcaggtc
     8341 aggcgagtcc caataccaaa acttaattgc acgcgggaag agaagtggcg gtatagctca
     8401 accgctttgc gtaaatccag attgtcagag aaaaccagcg ttttactctg tggatcaatt
     8461 cccagctttt cataatgtgc aatggctttt tcaccccatt caaccgggtc accagagtca
     8521 tggcgcaggc cctgatagcg actggcgaac tcgacaccga aatctcgcag gaaagcatcc
     8581 atagtgatgc agtcggttaa cgcaatgcca agttggtcgg gatactcttc cagccaggca
     8641 gcaagtgcag ctcgctggct gttggctaga tccgggctga tttgctgatg cgcctggaac
     8701 cattcgtgtg cctgtgttcc catcggcgtg agggaaagcc gacgcgccag atcgtagttg
     8761 ctggtgccca cgaaccagga ttcctgttgc agacgtttca cgatggtttc ttgtacttca
     8821 cgagaaaaac gtcggcgggt gccaaaatcc atcagatgga agcgcgacat atcaagaccg
     8881 gcggttaatg ccgagaagtc ggctaatttg ctttccagcg tgtcgagggc ttgcgcaacg
     8941 tcggcctgcg gtgagcgata gcgatgtacc atttcactga taaccgccag caaaggaact
     9001 tcccagagga tgacttcacg ccacgggccg cttaaacgaa tatccagctt gccattatcg
     9061 ttggacacgg tgacttgttc cgggttaaag cggaactcgc gtagccagtt aagatagtcg
     9121 gccttaaaga aaggcagggc agagagccac tgatattcat catcctgcag gcgcaggtac
     9181 tgcatcgcct gaacctgttc acgaatagca tcggcataaa tacccagcag atcgtcacct
     9241 cggcaacgaa actccgccgc gacatgcaca tcgtaatagt gatgaaacac ggcttgctgc
     9301 atatgcaact tataagcatc tgtatccagc aacgagtgca gaacaggaga agcgaattgt
     9361 gtcataggtg cgcagtagcg tcctcgtaca ggagcgttta gtacaataaa catcttcagg
     9421 aactgctgga gtataccttg tttcgcaatt tattgaaccc cgatcacacc atatgccacc
     9481 tttctggtcg atggcattac gcggtgtatg ttataaaaat gtagcaataa aggcgtttgt
     9541 acctgaaaag atgaagattc tgcatagcgc gatttacgca acaggaatag actgaacacc
     9601 agactctata aaagatgcta aaggttattt atgactcaac agccacaagc caaataccgt
     9661 cacgattatc gtgcgccgga ttaccagatt actgatattg acttgacctt tgacctggac
     9721 gcgcaaaaga cggtcgttac cgcggtcagc caggctgtcc gtcatggtgc atcagatgtt
     9781 ccgcttcgtc tcaacggcga agacctcaaa ctggtttctg tttatattaa tgatgagccg
     9841 tggaccgcct ggaaagaaga agagggcgca ctggtcatca gtaatttgcc ggagcgtttt
     9901 acgcttaaga tcgttaatga aattagcccg gcggcgaata ctgcgctgga agggctttat
     9961 cagtcaggcg atgcgctttg cacccagtgt gaagccgaag gtttccgcca tattacgtat
    10021 tatctcgacc gcccggatgt gctggcgcgt tttaccacca aaattattgc cgataaaacc
    10081 aaatatccct tcctgctttc caacggtaac cgcgttgcgc aaggcgaact ggaaaacgga
    10141 cgtcactggg tacagtggca ggacccgttc ccgaaaccgt gctacctgtt tgcgctggtg
    10201 gcaggcgact tcgacgtgct gcgcgacacc ttcaccacgc gttctggtcg cgaagtggcg
    10261 ctggagctgt acgtcgatcg cggcaacctc gaccgcgcgc cgtgggcgat gacctcgctg
    10321 aaaaactcca tgaaatggga tgaagaacgc ttcggcctgg agtatgacct cgacatctat
    10381 atgatcgtcg cggtggactt cttcaatatg ggcgcaatgg agaataaagg tctgaatatc
    10441 tttaactcca aatatgtgct ggcccgtacc gacaccgcca ccgataaaga ttacctcgat
    10501 attgagcgtg ttattggtca cgaatatttc cacaactgga ccggtaaccg tgtgacctgc
    10561 cgcgactggt tccagctcag cctgaaagaa ggtttaaccg tcttccgcga tcaggaattc
    10621 agctctgacc ttggttctcg cgcggtaaac cgtatcaaca acgtgcgcac catgcgcgga
    10681 ttgcagtttg cagaagacgc cagcccgatg gcgcacccaa ttcgcccgga tatggtcatt
    10741 gaaatgaaca acttctacac cctgaccgtt tacgagaagg gcgcggaagt gattcgtatg
    10801 atccacactc tgcttggcga agaaaacttc cagaaaggga tgcagctcta tttcgagcgt
    10861 catgatggta gcgcagcgac ctgtgacgac tttgtgcagg cgatggaaga tgcgtcgaat
    10921 gtcgatcttt cccatttccg ccgttggtac agccaatccg gcacgccggt tgtgactgtc
    10981 aaagacgact acaatccgga aactgagcag tacaccctga ccatcagcca gcgcacgcca
    11041 gctacgccgg atcaggcaga aaaacagccg ctgcatattc cgtttgccat cgaactgtat
    11101 gataacgaag gcaaagtgat cccgttgcag aaaggcggtc atccggtgaa ttcggtgctg
    11161 aacgtcaccc aggctgaaca gacctttgtt tttgataatg tctacttcca gccggtgcct
    11221 gcgctgctgt gcgaattctc tgcgccagtg aaactggaat ataagtggag cgatcagcaa
    11281 ctgaccttcc tgatgcgtca tgcgcgtaat gatttctccc gttgggatgc ggcgcaaagc
    11341 ctgctggcaa cctacatcaa gctaaacgtc gcccgtcatc agcaagggca gctgctgtct
    11401 ctgccggtgc atgtggctga cgctttccgc gcggtgctgc tcgatgagaa gattgatcca
    11461 gcgctggcgg cagaaatcct gacgctgcct tctgtcaatg aaatggctga actgttcgat
    11521 atcatcgacc cgattgctat tgccgaagta cgcgaagcac tcactcgtac tctggcgact
    11581 gaactggcgg atgagctact ggctatttac aacgcgaatt accagagcga gtaccgtgtt
    11641 gagcatgaag atattgcaaa acgcactctg cgtaatgcct gcctgcgctt ccttgctttt
    11701 ggtgaaacgc atctggctga cgtgctggtg agcaagcaat accatgaagc aaacaatatg
    11761 accgatgcgc tggcagcgct ttctgcggcg gttgctgcac agctgccttg ccgtgacgcg
    11821 ctgatgcagg agtacgacga caagtggcat caggatggtc tggtgatgga taaatggttt
    11881 atcctgcaag ccaccagccc ggcggcgaat gtgctggaga cggtgcgtgg cctgttgcag
    11941 catcgctcat ttaccatgag caacccgaac cgtattcgtt cgttgattgg tgcgtttgcg
    12001 ggcagcaacc cggcagcgtt ccatgccgaa gatggcagcg gttaccagtt cctggtggaa
    12061 atgcttaccg acctcaacag ccgtaacccg caggtggctt cacgcctgat tgagccgctg
    12121 attcgcctga aacgttacga tgccaaacgt caggagaaaa tgcgcgcggc gctggagcag
    12181 ttgaaagggc tggaaaatct ctctggcgat ctgtacgaga agataactaa agcactggct
    12241 tgatactttt gagccaaaat gccggatgcg acgcttgacg cgtcttatca ggcctacaag
    12301 tccgttgaaa atgctggttt tgtaggctgg ataaggcgtt cacgccgcat ccggcagccg
    12361 tgcccaaatg cctgatgcga cgcttgacgc gtcttatcag gcctacaatc cgttgaaact
    12421 gctggttttg tgtgccacat ccggcatatt aaccatgacg ccgaatcggt tgttcagagt
    12481 gcccacgctg catcacccgt tgcaaaactt ccgcttccag ctccgctaat cgcaccgacc
    12541 ctaaccggcg cggacggggg atatccaccg tcaaatccag accaattttt ccctcttcaa
    12601 ttaacaatac ccggtcagcc attgctaccg cttcgctcac atcatgcgtc accaacagta
    12661 cggtaaagcc gtgctcctgc caaagtgaca caatcaaatc ctgcatctcg agccgagtca
    12721 gggcgtccag cgccccgagc ggttcatcaa gcaataataa acccggtcga tgaattaacg
    12781 ctcttgccag cgccacacgc tgtttctgcc cgccagaaag tgcggcaggc cattcccctg
    12841 cgcggttctc cagtcctact gcagccaacg cccgacgcgc ggcatcgcgc cactggcctt
    12901 taaggcctaa cccaacgtta tcaatcaccg atttccacgg tagcagacgc gcatcctgaa
    12961 acatcattcg cgtgtcttcc tgaatctcag ccagcggtgt ggtgcccgct aacacgtcgc
    13021 ctgcggttgg cgtttccaga cctgccagca ggcgcagcag ggtacttttg ccaccaccgc
    13081 tgcggcccac caccgccaca aactgacctg ccggaatatg taaatccagt tggttcagga
    13141 cgatattttc cgcgtattgt ttgcttactg cattgagcaa caatggtgtg ccctggttca
    13201 gacgagcagt attcataccg tggcctcctt caaatgataa gccgggttcc agcgcaacca
    13261 gagacgctct aacagctgcg cgctgacgtc agcaagtttg ccgagcaggg cgtaaagaat
    13321 aatagcgact accaccacgt ccgtttgcag aaactcccgc gcattcatcg ccagataacc
    13381 aatgccggaa ttggcagaaa tagtttcggc aacgatcagc gtcagccaca tcaggcccaa
    13441 agcaaaacgc acgccgacca taattgaggg cagggcgcca ggcaggatca catggataaa
    13501 cagcggtatg ccggataatc catagctacg cgccatttcc accagaccgc gatcgatatt
    13561 acggatgccg tgccaggtgt tgatataaat ggggaatagc gtgccgagcg ccaccagaaa
    13621 gatttttgcg gattcatcaa tgccaaacca caaaatcacc agcgggatca gcgccagatg
    13681 cggcacgtta cgcaacatct gaatggaggt atccagcagt cgttctcccc agcgagatag
    13741 cccgctaatc agtcccaaaa tcaaccccag cgatccgcca attgaaaagc caatgagcgc
    13801 ccgccaggag ctgatcgcca gatgctgcca caattcgccg ctggcggaga gcgtccagaa
    13861 cgccatcacc actccttccg gtgagggcaa aatacgcgtt gacagccagc caactgagga
    13921 ggccagttgc cacaccgcca cgatgcccac cggtaaaaac cagggggcaa cgcgcaataa
    13981 ccactttttc actggcgttg ccattatcgt attccttagc tttgcgcgac gttacggggg
    14041 ataaaatcat ttgccaccgc ttcgccttgc ggattcagcg gctgcggctg gggaatttcc
    14101 gggatggcga catccagatg cgggaacagc aactcgccaa cccgatacgc ttcttccaga
    14161 tgcgggtagc ccgaaagcac aaagctgtcg atgccaagcg cggcgtattc gttgattcgc
    14221 gcagcgaccg tcggaccatc gcccaccagt gccgtcccgg caccgccgcg cactaagcca
    14281 acgcccgccc ataaattggg gctgatctcc agattgtcgc gcttgccgtt atgtagcgcc
    14341 gccattcgct gttgtcctac ggaatccgtc cgggcgaatg cggcttgtgc tttggcgata
    14401 gtctcatcat caagatgcga gattaaccgc tcggcggcct gccacgcttc gtcgttggtt
    14461 tcacgaacaa tcacatgcag acgaataccg aaacgaattt tgcgtccatg cgcggcagct
    14521 ttcgcccgca cttgttcgat tttctcttta accagttccg gcggttcgcc ccaggtgagg
    14581 tagagatcaa cttgttctgc cgccagctcc tgggcgacat ctgacgatcc gccaaagtaa
    14641 agcggaggat acggctgttg aatcggcggg aagagcagtt tggctccgcg cacatgaata
    14701 tgtttaccgt tgaaattgac ggtttccccc agcaataagc gccgccagac ctgggtaaat
    14761 tccgccgagg cttcgtagcg ctcgctatga tcaaggaaca cgccgtcgcc tgccagctct
    14821 tgtggatcgc tgcctgtgac cagattaaac aacgcacgac cattcgagag acggtcaagc
    14881 gtggcggcct ggcgggcggc aacggtaggt gaggttacgc tgggacgcag ggcgacaaga
    14941 aacttcagcc gttgcgtcac cgggatcatc gacgcggcaa ccagccacgc atcttcacac
    15001 gatcgtcctg ttgggatcag cacaccggta taacccagac gatccgccgc ttgcgcaatt
    15061 tgttgcagat aaccgtgatc aaccggacgt gaaccttctt ccgttcccag atagtgcccg
    15121 tcaccatggg tcggtaaaaa ccagaacata ttcagactca taattgtttt ccttccagtt
    15181 gagtgggctg ccagatgcgc tggcgaatat cgactttttt cggcaccaga cggttttcat
    15241 aaaacagatc tgccgtttgc tgctgtaagg cggcaacttc agcgctcaac ggtttgatgg
    15301 tggtgggagg acgatgatcc agatatgagg caataaccgg tgccggtaag cccatcgttt
    15361 ttgccagtaa agcgatgctt tgttcgcgct ggctgcgggt taacgcatcg gcctcactaa
    15421 aggttgtcag cacaccctga ataaaagcgc cgtttttttc tgcatacgga cgagctgcca
    15481 gataaaacga tccagtttga ttgagatcgg tgccgtcttt cagcacccgc acgccgccct
    15541 gtaataatgc agcggagtag tagggatccc agatagccca ggcgtcaacg ttaccttgct
    15601 ggaacgcggc gcgggcatcg gcaggcgtca ggtaagtggg ctgaatatcc gtgaatttaa
    15661 gcccggcctg gcgaagggct cgcagtaaaa gattgtgcga actggaacct ttctgaaagg
    15721 caactttgtg acctttaaga tcggctacgg ttttgatcgg gctgttttct gccaccagaa
    15781 tcacttcggc tttgggtttc ggtggctcga cgcccacgta taccaaatca gccccggcag
    15841 cctgggcaaa gattggcgga atatccccgg tactgccgag atcgatactg ccaacgttta
    15901 acgcctccag catctgtggg ccagcgggga actccaccca ggagattttt gtttgcggat
    15961 agcgtttttc cagtaactgg tggctttttg ccagcaccat accaatactg cctttttgat
    16021 agcctatacg taacgcctct ggcgaagact ccgcggcaac agcaagtgta gagacgctaa
    16081 gtaaacccgc cagcgccagt ttaatgatct tacgcatggg cattacctcg cagagacaga
    16141 aggtcaggaa cctgaacatc gcggcggtgc aacgcctgcc agaaagtttc tagcgcggta
    16201 tcaagacgag tttgcagatt tggcgtgaac tggggtttgt gttggtaatc aattacttgc
    16261 gagtcatcgg caaacacgcc gtgcaggatc tcctgggctt tcagcgtact taaaactggc
    16321 ttaagggcat aatcgaccgc cagcagatgg gccacggtgc cgcctgtcgc cagcggcagc
    16381 accactttgc cttgcaacgc acgttctggc agcaggtcga gcagggtttt cagcgcgccg
    16441 gaataggcgg ctttatacac aggcgtggcg acaatcagcc catcggcctg ttgcagctgt
    16501 tcggtgaagg tcttgagtgc cggactatcg aaacgagcat aaagcagatc ttccgggacg
    16561 aagttttgca aattccagtg ataaacctct acatccaggc catttagttt ttcccgcgca
    16621 tattccagca aggagctgga gcgagaagga aagcgaggac tacccgccag ggtgatgaca
    16681 cgcatactct ctccttataa ccaattgttc ttgttttgtt aacattgata acaattcggt
    16741 cagtctgaca gagggcgtcc gcgagtcgaa atgatttatc tgaggaagaa aagccaaaaa
    16801 aagcataaaa aaagcgcaat aagaaaacgt ttgctattta ttgccgcagg tcaattccct
    16861 tttggtccga actcgcacat aatacgcccc cggtttgcac accgggaatc caggagagtt
    16921 catgtactac cccttcgttc gtaaagccct tttccagctc gatccagagc gcgctcatga
    16981 gtttactttt cagcaattac gccgtattac aggaacgccg tttgaagcac tggtgcggca
    17041 gaaagtgcct gcgaaacctg ttaactgcat gggcctgacg tttaaaaatc cgcttggtct
    17101 ggcagccggt cttgataaag acggggagtg cattgacgcg ttaggcgcga tgggatttgg
    17161 atcgatcgag atcggtaccg tcacgccacg tccacagcca ggtaatgaca agccgcgtct
    17221 ctttcgtctg gtagatgccg aaggtttgat caaccgtatg ggctttaata atcttggcgt
    17281 tgataacctc gtagagaacg taaaaaaagc ccattatgat ggcgtcctgg gtattaacat
    17341 cggcaaaaat aaagatacgc cggtggagca gggtaaagat gactatctga tttgtatgga
    17401 aaaaatctat gcttatgcgg gatatatcgc catcaatatt tcatcgccga ataccccagg
    17461 actacgcaca ctgcaatatg gcgaagcgct ggatgatctc ttaaccgcga ttaaaaataa
    17521 gcaaaatgat ttgcaagcga tgcaccataa atatgtgccg atcgcagtga agatcgcgcc
    17581 ggatctttct gaagaagaat tgatccaggt tgccgatagt ttagttcgcc ataatattga
    17641 tggcgttatt gcaaccaata ccactctcga tcgttctctg gttcagggaa tgaaaaattg
    17701 cgatcaaacc ggtggcttaa gtggtcgtcc gcttcagtta aaaagcacag aaattattcg
    17761 ccgcttgtca caggaattaa acggtcgctt accgatcatc ggtgttggcg gcattgactc
    17821 ggttatcgct gcgcgtgaaa agattgctgc gggggcctca ctggtgcaaa tttattctgg
    17881 ttttattttt aaaggtccgc cgctgattaa agaaatcgtt acccatatct aattatttct
    17941 tcgaattcct ctttataaga taatcagggc tttattttaa gccctggttg ttttatattc
    18001 atcgctgttg cttatttaga cattttgtac ttttattgtt gaggttatta agcgaagcga
    18061 caatggattg tgttgctgcg gttttatagg gtaggggaga ggcaaatgcg aattaaacca
    18121 gacgataact ggcgttggta ttacgatgaa gagcacgatc gaatgatgct cgatttagcc
    18181 aatggtatgc tatttcgctc acgttttgcg cgcaaaatgt tgactccaga tgccttttct
    18241 cccgcaggct tttgcgttga cgacgccgcg ctctatttct catttgaaga aaaatgccgc
    18301 gattttaatt tatctaaaga acaaaaagcc gaactggtgc ttaatgcact ggtagcaatt
    18361 cgttatctca aaccgcaaat gccgaaaagc tggcattttg tttcccatgg tgaaatgtgg
    18421 gtgcccatgc cgggcgatgc ggcctgcgtt tggttgagcg acactcacga gcaagtcaat
    18481 ttgctggtcg ttgaatctgg cgaaaatgcc gcgctatgtc tgctagccca accctgcgtt
    18541 gttattgcgg gtcgcgcgat gcagctgggc gacgccatta aaatcatgaa cgacaggctg
    18601 aaaccgcagg tgaatgttga cagcttcagc ctcgaacagg cagtctaacg cgccaactta
    18661 agtgcagtct tcggtacaca gctacagcaa agaatggtgc catcatcgcc cattgctgat
    18721 tttttcagcg gcgtgacttc gccttctaaa agctgaacac ggcaacttcc acaaatgccc
    18781 gcgcggcaag aataagggat acgaattccc tgattttcta attgctccag caacacctgt
    18841 tggttatttc cacgaaatgc ctgtccctgc cagtcaatat ctacgtttgc gtccggttgt
    18901 tgcgtgatgt tggcagtatc atcagcggca gctgcgccgt aaattttagc cggagccgtt
    18961 gccagtattt ctacctcatc gccaacgcga atcacgccgc tattacgggc aattaaattc
    19021 tgaccaaaat cgacatcgcc gttatcctgg gcagtgcgga aagattgcaa tgtttttaat
    19081 ggttcgcctg ccggatgttt ttgccctttt tctgggctga cggtggtgaa aatacagcgg
    19141 ctacaaggtt taaccacatc aaataccaca tcaccaatgc gaatcacttt ccagctatct
    19201 tcttcccagg cggacgcgcc ggaaaccacc agattagggc ggaattgctc catttttaca
    19261 ctggccggac aacgttgttg gaggtcacgt aaggaagctt cgttagcaag aaggtaagga
    19321 tagccatcag caaatgacag gggtacagtg ttgtggcgtt tcacgcgccg cgtcatttgt
    19381 ggccccaccc agcgcaactg cacttcgcgg gagaaaaatc cacttagcca tttgttgatc
    19441 gcgtctggcg caattcgcgc ggtaaaatgt gtgccccaaa cttcggttgg tgcgtcttgt
    19501 atggcgaaat cagcaaaacg aacatatgcg ctactgccat ctggtgcggt gagatgcaag
    19561 ccatcatgca caggtgaagg agtaaaccgt accatctggg gaaactggcg agcggtaata
    19621 aacgtaccgt caggttccgt gatcataaag atgcgatcga aggccagacc actgacatca
    19681 gccagagcat gtgtaagacc aatgccgcgc atcgatttaa caggatgaat aaaaagccgg
    19741 gttaatgtcg ccacggtgcg gtcctcaaat gaaaataagc cctcaacttt atgacatgac
    19801 gcgcttatta gctataatgc gcaacaattt tcttagcgcc tgtcccatta ggctatttta
    19861 tttgtcagtt tggccctggg ttgtgctcga aatcctcacg tacttgtgta cgctccggtt
    19921 tctccgcgct gtccatgtcc agactgactg caacaattac gcctactgcg gtaggctctt
    19981 agagtaaaag tgacgatatg aattctctgt ttgccagtac ggcccgtggg ctggaagagc
    20041 tgttaaaaac tgaactggaa aacctggggg ccgttgaatg ccaggtggtt cagggtgggg
    20101 tccatttcaa gggcgacaca cggcttgttt accagagcct gatgtggagc cgcctggcct
    20161 cgcgtattat gttgccgctg ggcgagtgta aggtttacag cgatttagac ctctatctcg
    20221 gtgttcaggc gatcaactgg acagagatgt ttaatcctgg tgcgaccttc gctgtccact
    20281 tcagtggttt gaatgacacc atccgcaaca gtcagtacgg tgcgatgaaa gtgaaagacg
    20341 cgatcgtcga tgctttcacg cggaaaaatc tgccgcgtcc aaatgttgat cgcgatgcgc
    20401 cggatatccg cgttaacgtc tggctgcata aagaaactgc cagtatcgcc cttgatctca
    20461 gtggtgatgg tttacatctg cgtggctatc gcgatcgtgc tggtattgcg ccgatcaaag
    20521 aaaccctggc agccgctatt gtgatgcgat ccggctggca gccaggaaca ccgctgctcg
    20581 atccaatgtg tggttctggt acgttgctga ttgaagctgc gatgctggcg accgatcgcg
    20641 caccaggttt gcaccgtggg cgttggggct ttagcggctg ggcgcagcat gatgaagcta
    20701 tctggcagga agtgaaagcg gaagcgcaaa ctcgcgcccg taaaggcctg gctgagtata
    20761 gctctcattt ttacggttcg gacagcgacg cacgggtgat tcaacgtgcg cgcactaacg
    20821 cccgtcttgc ggggattggt gaactgatca cctttgaggt gaaagatgtc gcgcaactga
    20881 ccaatccgct gccgaaaggg ccgtacggta cagtgttgag caacccgcca tacggtgaac
    20941 gtctggacag cgaaccggcg ctgattgcgc tacatagtct gctgggccga atcatgaaaa
    21001 accagttcgg tggctggaat ctctctttgt ttagtgcctc gccggatctg ctcagctgcc
    21061 tgcagctgcg tgcagacaaa cagtacaagg cgaaaaacgg cccgctggac tgcgtacaga
    21121 aaaattacca cgttgccgaa agcactccag acagcaaacc ggcgatggta gcggaagact
    21181 acgctaaccg cctgcgtaag aacctcaaaa aattcgagaa gtgggctcgc caggaaggga
    21241 ttgaatgtta ccgcctgtat gacgccgatc tgccggaata taacgttgcc gttgatcgtt
    21301 atgccgactg ggtggtggtg caggagtatg cgccgccaaa aactattgat gcgcacaaag
    21361 cgcgtcagcg tctgttcgat attatcgctg caaccatttc ggtactgggg attgcgccaa
    21421 acaaactggt gctgaaaacc cgtgaacgtc agaagggcaa aaatcagtac cagaaactgg
    21481 gcgagaaggg cgagtttctg gaagttaccg aatataacgc tcacttgtgg gtgaacctga
    21541 cggattatct cgatactggt ctgttcctcg atcaccgcat cgcccgtcgt atgctcggtc
    21601 agatgagcaa aggcaaagat tttctcaacc tgttctctta taccggcagt gccaccgtgc
    21661 atgcgggatt aggcggtgca cgcagcacca ccaccgtgga tatgtcgcgt acttatctgg
    21721 agtgggcaga acgcaacctg cgtctgaatg gcctaaccgg gcgtgcgcat cgcctgattc
    21781 aggccgattg cctggcgtgg ctgcgtgagg caaatgaaca gtttgatctg atctttatcg
    21841 atccgccaac cttctctaac tcaaaacgaa tggaagatgc gtttgatgtt cagcgcgatc
    21901 atctggcgct gatgaaagat ttgaaacgtc tgctacgtgc tggtgggacg atcatgttct
    21961 cgaacaacaa acgtggcttc cgtatggatc ttgacggact ggcaaaactg ggactgaaag
    22021 cacaagaaat tacgcaaaaa acgctctccc aggattttgc ccgtaaccgc cagatccaca
    22081 actgctggct gattaccgca gcctgaaagg aatagtaatg tcattaatca gtatgcatgg
    22141 cgcatggctg tcgttcagcg acgcgccgct tctcgataac gcagaactgc atatcgaaga
    22201 taacgaacgt gtttgtctgg taggccgtaa cggcgcaggc aaatcgacgt taatgaaaat
    22261 cctcaaccgt gaacaagggc tggatgacgg tcgcattatt tacgagcaag atttgattgt
    22321 agctcgtctg caacaggatc cgccgcgtaa cgttgagggg agtgtttatg atttcgttgc
    22381 cgaaggcatt gaagaacaag cggaatacct gaaacgctat cacgatattt cgcgcctggt
    22441 gatgaacgac ccgagcgaga aaaatctcaa cgaactggcg aaggttcagg aacagttgga
    22501 tcaccacaac ctgtggcagc tggaaaaccg cattaacgaa gtgctggcgc aactggggtt
    22561 agatcctaac gttgcgctgt cgtcgctttc cggcggctgg ttgcgtaaag cggcattagg
    22621 acgcgcgctg gtgagcaatc cgcgcgtatt gctgctcgac gaaccgacta accacctgga
    22681 tatcgaaacc atcgactggc tggaaggatt ccttaaaacc ttcaatggga cgataatttt
    22741 catctcccac gaccgttcgt ttatccgcaa tatggcgacg cgcattgttg atctcgatcg
    22801 cggcaaactt gtgacctatc cagggaatta cgaccagtac ctgctggaaa aagaagaagc
    22861 tttgcgtgtg gaagaactac aaaatgccga gttcgatcgc aaactggcgc aggaagaggt
    22921 gtggatccgc caggggatca aagcacgccg tacccgtaat gaaggccgcg tacgcgccct
    22981 gaaagcaatg cgtcgcgagc gtggcgaacg tcgcgaagtg atgggtaccg caaagatgca
    23041 ggtggaagag gccagccgct ccggtaagat cgttttcgaa atggaagacg tttgctacca
    23101 ggtcgacggt aagcaactgg tgaaagattt ttctgcccag gttctacgtg gcgacaaaat
    23161 tgccctgatt ggtccgaatg gatgcggcaa aaccacgctg ctgaaactga tgctcggtca
    23221 gcttcaggcg gacagcgggc gtattcacgt tggcaccaaa ctggaagtgg cttatttcga
    23281 tcagcaccgt gcggaactgg atcccgataa aacggtgatg gataaccttg ctgaaggtaa
    23341 gcaagaggtg atggttaacg gcaagccacg tcatgttttg ggctatttgc aggacttcct
    23401 gttccatccg aaacgggcga tgacgccggt acgtgcgctt tctggcggtg agcgtaaccg
    23461 cttgctgttg gcgcgtttgt tcctcaaacc aagcaactta ttgattcttg acgaaccgac
    23521 caacgatctt gatgttgaaa cgctggaact gctggaagaa ctgatcgaca gctaccaggg
    23581 cacggtattg ctggtgagcc acgatcgtca gtttgtcgat aacaccgtta cagaatgctg
    23641 gatcttcgaa ggcggcggta aaattggtcg ttatgtcggc ggttatcatg atgcccgtgg
    23701 tcagcaagaa cagtatgtgg cgctcaaaca gcctgcggtg aaaaaaatcg aagaagccgc
    23761 cgcgccaaaa gcagaaactg taaaacgcag cagtagcaaa ctaagctata aattgcagcg
    23821 cgaactggag cagctaccgc aattgctcga agatctggag gcgaagctgg aagccctaca
    23881 gacgcaggtg gcggatgctt ccttcttcag tcagccgcat gagcagacgc aaaaagtgct
    23941 cgccgatatg gctgctgcag agcaggagct ggagcaagcc tttgaacgct gggagtatct
    24001 tgaagcgtta aaaaatggtg gctgatcgca aaatagtcga tttaaacaga gacagctgac
    24061 aacggtaaaa ttgcctgatg aactgggttt atcaggcatg aaaaccaaca ctttgttagt
    24121 catctgtaaa atagcgcatc attaaggagt accaatgtgc gaacatcatc atgccgcgaa
    24181 gcacatcctg tgctcgcagt gtgacatgct ggtggcgtta ccgcgccttg agcatggtca
    24241 gaaagcggca tgtccccggt gtggcacaac gttaaccgtg gcgtgggatg cccctcggca
    24301 gcgtcccacc gcctatgcgt tggctgcact gttcatgctg ttgctgtcca acttgtttcc
    24361 ttttgtgaat atgaacgttg caggagtcac cagtgaaatt acattactgg aaattcccgg
    24421 cgtgcttttt tctgaggact acgccagcct cggcaccttt ttcctgttgt ttgtgcaact
    24481 ggttcccgcg ttttgtctga taaccattct gttactggtg aatcgcgcag aattaccggt
    24541 ccgtttaaaa gagcaactgg cacgggtgct ttttcaactc aaaacctggg gaatggcgga
    24601 gattttcctc gcgggtgtgc tggtcagttt cgttaaactg atggcctacg gcagcattgg
    24661 ggtaggcagc agctttctcc cctggtgttt attttgtgtc ctgcaactgc gcgcttttca
    24721 gtgcgttgat cgtcgctggt tatgggacga catcgccccg atgccagaac tgcgccagcc
    24781 gctaaaacca ggcgtcacgg ggatacgtca ggggctgcgt tcgtgctcct gttgtacggc
    24841 aatccttcct gctgatgaac ccgtgtgccc gcgttgtagt accaaagggt acgttcggcg
    24901 tagaaacagc ctgcagtgga cactcgcgct gcttgtaacg tccatcatgt tgtatcttcc
    24961 ggctaatatt ttgcccatta tggtgacgga tttattaggc tcgaagatgc cgtcgacgat
    25021 tctcgctggg gtcattctgt tatggagcga aggatcttat cccgtcgctg cggttatctt
    25081 tctggccagt attatggtgc caacgttaaa gatgatcgcc atagcgtggt tgtgttggga
    25141 tgccaaaggg catggcaagc gcgacagtga aagaatgcat ttgatttatg aagttgttga
    25201 gtttgtaggc cgctggtcga tgattgacgt tttcgttatc gcggtgctct cggcgctggt
    25261 gcgtatggga ggtttaatga gtatttatcc ggcaatgggt gcattaatgt ttgctttagt
    25321 cgtcataatg acaatgtttt ctgctatgac gtttgacccg cgtttgtcgt gggatcgtca
    25381 acctgaatca gagcatgagg agtcctgaca gtatggaatc taataatggg gaagccaaaa
    25441 tccagaaagt gaagaactgg tctcccgtgt ggatatttcc tatcgtcacg gcgctcattg
    25501 gggcctgggt tctattttat cattacagcc atcaggggcc ggaagtgacc ctgatcaccg
    25561 cgaatgcgga aggaattgaa ggtggtaaaa ccaccattaa aagccgtagc gttgacgtcg
    25621 gcgtggttga aagcgccaca ctggctgatg atttgacgca cgttgaaatc aaagcgcggc
    25681 tgaattccgg tatggaaaaa ttgctgcata aagataccgt cttttgggtg gtgaaaccgc
    25741 agattggtcg agaagggatt agcggcctgg gaacgctgct gtctggggtt tatatcgaac
    25801 tgcagccagg cgcgaaaggc agcaaaatgg ataaatacga tttgctggac tcgccaccgt
    25861 tggccccgcc tgatgcgaaa ggtatccgtg tgattctcga tagcaaaaaa gccgggcagc
    25921 tctcgccagg agatcccgtg ctgttccgtg gctatcgggt aggttcggtt gaaaccagca
    25981 ccttcgatac gcaaaaacgc aatatcagct atcaactgtt catcaatgca ccttatgacc
    26041 gactggtgac cagcaatgtt cgcttctgga aagatagtgg cattgcggtt gatttgacgt
    26101 cagcgggaat gcgtgtggag atgggctcat tgacaacgct gcttagtggc ggtgtcagct
    26161 ttgatgtgcc ggaaggtctg gatttagggc agccagtggc accgaaaaca gctttcgttt
    26221 tgtatgatga tcagaagagc attcaggatt cgttgtacac cgatcacatt gattatctga
    26281 tgttctttaa agattcggta cgcggtctgc aaccgggagc tccggtagaa ttccggggta
    26341 ttcgcctggg taccgtaagc aaagtgccat tctttgcgcc gaatatgcgt cagacattta
    26401 acgatgatta tcgtattccg gtactgattc gtatcgagcc agagcggctg aaaatgcagc
    26461 ttggcgaaaa tgcggatgtt gttgagcacc ttggcgaatt gttgaaacgt ggtttacgcg
    26521 gatcgctgaa aaccggaaac ctggtcactg gcgcactgta tgttgatctc gatttctatc
    26581 caaatacgcc tgcaataacc ggtattcgtg aatttaatgg ttatcagatt atcccgaccg
    26641 tcagcggcgg cctagcgcaa atccagcaac gactgatgga agcgttggat aagatcaaca
    26701 aactgccact gaatccgatg attgaacagg caaccagtac gctttctgaa agtcagcgca
    26761 caatgaaaaa cctgcaaacg acgctggata gcatgaacaa gatccttgcc agccagtcga
    26821 tgcagcagtt accgacggat atgcagtcaa cgttgcgtga attgaatcgc agcatgcagg
    26881 gcttccagcc tggttccgca gcctacaaca agatggtggc ggatatgcag cgccttgatc
    26941 aggtgttgcg agaactgcaa ccggtgctga aaacgctcaa cgagaagagt aacgcgctgg
    27001 tatttgaagc gaaggacaaa aaagatccag agccgaagag ggcgaaacaa tgaaaaagtg
    27061 gctagtgacg attgcggcac tgtggctggc cggatgcagc tccggcgaaa ttaataaaaa
    27121 ctattaccag ttacctgtgg tgcagagcgg tacacaaagc accgccagcc agggtaatcg
    27181 cctgttatgg gttgagcagg tcgctgttcc tgactatctg gcggggaatg gtgtggttta
    27241 tcaaaccagt gatgtgaagt atgtgattgc caacaacaac ctgtgggcca gcccgttgga
    27301 tcaacagttg cgcaacactc tggttgccaa cctgagcacg caactgcccg gctgggtggt
    27361 tgcctcccag cctctgggaa gcgcccagga tacgctcaat gttaccgtaa cggagtttaa
    27421 cgggcgatat gatggcaagg tcattgtcag tggtgagtgg ctgttgaacc accagggaca
    27481 actgatcaaa cgtccgttcc gtctggaagg ggtgcaaact caggatggtt acgatgaaat
    27541 ggttaaagtg ctggctagtg tctggagtca ggaagccgct tctattgcgc aagagataaa
    27601 gcgtctaccg taatcataaa gatttgtaaa tataaccgtc tccggtatgt tgcctgaggc
    27661 ggtttttttg tttctaaggt gcggaaaaag ttgttcctct tcacattttt tgtacaactg
    27721 acatgcccgt gtagctcaca aatatgacag tggcgtgaat tttgcgcatt gacggcagtt
    27781 atgattcgcg gtattgctta actgtgattg cacatttagt aatcactgtt ttcttttcca
    27841 ccagaaacca gtatgaggga aacgaggcat gaagagacaa aaacgagatc gcctggaacg
    27901 ggcacatcaa cgtggttatc aggccggcat cgccggacgc tcaaaagaaa tgtgtcccta
    27961 tcagacgctg aatcaaaggt cacaatggct gggaggctgg cgagaagcca tggcggacag
    28021 ggtagtaatg gcctgattct gtctctttaa aaagaaacct ccgcattgcg gaggtttcgc
    28081 cttttgatac tctgtctgat tataatcaga aggcagacgt atcctggaac agaccgactt
    28141 tcaggtcgct ggcggtatag atcagacgac catcaaccag cacttcgcca tctgccagac
    28201 ccataatcag acgtcgatta acaatgcgtt taaagtgaat acggtaggtc acttttttcg
    28261 ctgtcggcag tacctgaccg gtgaatttca cttcgccaac gcccagcgcg cggcctttac
    28321 cttcgccgcc cagccagccg aggtagaacc ctaccagctg ccacattgcg tccaggccca
    28381 ggcatcccgg cataaccgga tcgccaatga agtggcatcc gaagaaccac agatccggat
    28441 tgatatccag ttctgcttca acataccctt tgtcgaagtt accacccgtt tcggtcattt
    28501 tgaccacacg gtccatcatc agcatgttcg gtgctggcaa ttgcgggcct ttagcgccaa
    28561 acagttcacc gcgaccagag gcaagaaggt cttcttttgt ataggattcg cgtttatcta
    28621 ccatgttctc tgtaagcctt attttattga agcacgcagg atagctaaca cgtgtacgct
    28681 gaacaagtcc gatcagttcg gaataaacca gttcagccaa cgcaatggcc atggaaaacg
    28741 gtgacgtcct tcctgttgcg atgcttgcgc gatacgttcc tggatggttt gcatcagcgt
    28801 cgtttggcct tcgccatccc acaccagatt taataataac ggcagtgcgt cagtcacatc
    28861 gtctactgcc cagatggtga atttgccttc ttctaccgct ttcaccagtt cactgtgaag
    28921 acttaaatgg cgaatgttag cagtggggat aatgacacct tgtttcccgg ttaactcacg
    28981 ttgctggcaa atagcaaaga agccttcgat tttctcattt aaaccaccga ccggctgggc
    29041 acgaccgaac tgatcgactg aacctgtgat agcgatactc tgattcaccg gcacatcggc
    29101 gagggcgctt atcagggcgc agagttcagc catcgaggca ctatcgccat ccacttcact
    29161 gtatgactgc tcaaatgtca gcgatgctga gaaggggatc tgttgctcaa gctgtagttc
    29221 cgacatcagg aacgcctgca tgatcatcat ccctttcgca tggatattgc cgccaagctc
    29281 cgctttgcgt tcgatgtcgg tgaactcacc atcgccaata tgcacaacgc agctaatgcg
    29341 agaaggttcg ccaaaagcgc gtggatgacc cggaaattca atgaccgaaa gggcgttaat
    29401 ttgcccgatg cgttcgcctt cggtttcaat caggatttgc tcctgaagga tctcatcctg
    29461 catgcgttca gcgaggaaac cttcacgcca ttcacgctgc tgcagcatta agtttagctg
    29521 ctcgccggag aaggtgtcgc catcgcacag ggaggcgacc tctttacact ggcggaggat
    29581 ccactgcggg ctaagcggaa gtgtttcttg ttcaccggtg tagcgggctg cttcgcggat
    29641 aagtaccggc caggcatccg ctcccggtgc aggtaagtga ttatgtcttg cggtcaatgt
    29701 tacccagcga caccactggc ttactgactc cgcatcgaca atctgcagag tatcttcaaa
    29761 ttcgctataa atagcctgct ctgaaagctc tggttccatc tcctggaaat cagccaatga
    29821 ttcgcgttcg cctaccagaa tgactttcag cttcaatggc atcgaaggca cagagacggg
    29881 gagagggcgc gactcatcaa acgcaaccca gtcaaaacgc tcgcggttaa cgatattttt
    29941 cagccgcatc cacagcagag gttgcgccag cagtgtacgc aaagagatga tgagaatacc
    30001 gccatttgcc tgatgcacca gacctggctg cagggtaatg tcgccattaa actggcgcag
    30061 gcagccaaag agttgttccg cttctaccca gtcggcagcg acaacttgcg ttaaagtcgc
    30121 aaaattatca tctgcactca ctgcgttacg taagcggatg gtgtgcccgg aaacctcata
    30181 gtgaccgccc accagttggc ctgcatcgct ttgtagcgca cgcgcggcat cggcaataag
    30241 attcagatac tcagactctt ccggggcctt cgccagcata aaagaggagg atgctcgcgt
    30301 atgcagcaac tgctccagcg caaattgcag tcgcggttga gtatcactga ataaaggatc
    30361 gttttcgtca atcaaatgtg gctgagcaaa tatttcctga tagctatcgg tatcaggaac
    30421 caggtcacgc catgcaagtt tcgtaatggt caaagttgat gttttttagt ctgttgtcaa
    30481 agccgcgatt ataccgtaac cggcactaca gcacacgtag aaagcaccga caatactcct
    30541 ggcatgggcg ttaaagctca caggatggag attcttttct tcactggccc aaaaagctga
    30601 tattctgtaa agagttacac ggtaacattg agatcgctat gaaatatcaa caacttgaaa
    30661 atcttgaaag cggttggaaa tggaagtatc tggtgaagaa gcaccgcgaa ggggagttaa
    30721 tcacccgtta catagaagcc agtgccgccc aggaagccgt cgatgtgttg ctctcgctgg
    30781 aaaatgaacc cgtgctggta aatggctgga ttgacaagca tatgaatccg gaactggtca
    30841 atcgcatgaa gcaaaccatt cgggccagac ggaagcgcca ttttaatgcg gaacaccagc
    30901 atacgcgcaa aaaatcgatc gatctggaat ttattgtctg gcaacgtctg gctggtcttg
    30961 cgcagcgtcg cggtaaaacg ctttctgaaa cgattgttca gctgattgaa gatgcggaaa
    31021 acaaagagaa atacgcgaat aaaatgtctt ctttgaagca ggatctgcag gcattgctgg
    31081 gtaaggaata actgacgaaa gtcagttcaa ttttctaaag acgaaaaaaa accccgcagc
    31141 agcggggttt tttaccagac gagaacttaa gcctgcggct gagttacaac gtctttgatg
    31201 cctttaactt cgatctctac gcgacgatcc ggagccaggc agtcgatcag tgcagcacgc
    31261 tgtttcacgt tgtcacaggt gttgccagta accgggttgg attcgcccat accacgtgcg
    31321 gagatcttgt ctgccgggat acctttggag atcaggtaat caacaacaga ctgagcacgg
    31381 cgctcggaca gaccctggtt gtaagcgtca gaaccgatgc ggtcagtgta acccagaaca
    31441 actacggaac cgtctttcgg atccaggttg ctcagctggc tgtacagctg atccagagca
    31501 gcctgacctt ccggtttcag ggttgctttg ttgaaggtga acagaacgtc agacttcaga
    31561 gtgaagtgct tggtctgtac ttccggtgcc ggagctggag ccggagcaac tactggagct
    31621 gcttcgccct gaccgaaacg gtaggaaaca cccaggctca gcatgccgtt gtccggacga
    31681 gtaccgatgg tgtgtgcgtc accgatgttg ttggtccact ggtattccag acgggtagcg
    31741 atttcaggag tgatcgcata ctcaacaccg ccagcgaaga ccggagaaac gccggtatcg
    31801 tggtttttac catcaaagtt ggatttagtg tctgcacgcc ataccatacc acccagacga
    31861 gtgtatacgt ccagatcgtc agtgattggg taacccagtt tagcggtcag ctgaacgccc
    31921 tgagctttat atgcaccgtt ttcaacgctg cctttgtatg gcatacgacc taaccagtca
    31981 taacccattt caaagccaac atacgggtta acctggtaac caccaaaagc accagcgccc
    32041 agttggtttt catgggtcgg accattgttg ttgatgaaac cagtgtcatg gtactgggac
    32101 cagcccagtt tagcaccagt gtaccaggtg ttatctttcg gagcggcctg cgctacggta
    32161 gcgaaaccag ccagtgccac tgcaatcgcg atagctgtct ttttcatttt ttgcgcctcg
    32221 ttatcatcca aaatacgcca tgaatatctc caacgagata acacggttaa atccttcacc
    32281 gggggatctg ctcaatatta actctaccga tatcttcggc ttatgccgag cacccctggc
    32341 gatgtaaagt ctacaacgta gttgaaaact tacaagtgtg aactccgtca ggcatatgaa
    32401 aaaaaagtct tgtataaggt acgtttaatc ttttttgtca gcgacaattt acagaaaaaa
    32461 atcgcggaaa ccgcttcaga caagcctccg caaggaaaat tagtcacgac tgaaagcatt
    32521 ggctgggcga caaaaaaagt tccaggatta atcctaaatt tacttaatga tacaaattag
    32581 agtgaatttt tagcccggaa agttgtctcg ttgcgtgaga ggaagcgctt accggacgca
    32641 taataaaccc catagcgtta ccttcatttg ccgcatcaac aagttcagca tgctcttctt
    32701 cagtcaaatc atctgccaac caaccgatca ccacactgta attgcccgtg cgtaaagcgc
    32761 gaaccattga ctccacggtg tggcaagggg agagctggtt aatctgcatt actttcgtta
    32821 agggtagccc agatgcctga acccattccc gactcagttt ttgttgcggt gttaaccaga
    32881 gttgccagcg cgattgctga ccgagttgct gtaacaatgg caacagtaga agttgcgtca
    32941 tcatgggctg atcttcgcga tagacaactt cactgataag cccggctgta gtgttttccg
    33001 tagagacacg cgcaatttta cttgctgcgg atgagaacga cgaagaacga tgtgcatagc
    33061 ctgaagtgta cataatcaat ccagcccctg tgagttactg tatggatgta cagtacatcc
    33121 agtgacgaca aagatcaacc ctattttcgg aaagagcctc gcaaattttg tcgttagtga
    33181 cgggaaaaca taaattaatc ttgcccctta agaataagtt gcctattttc gtagttaacg
    33241 gatccgttaa tgtgaatcat tcttttatgt tatgatttta aaaggaattt tatgaaaagc
    33301 ctctcctata agcggatcta taaatcacaa gaatacctgg caacgttggg cacaattgaa
    33361 taccgatcat tgtttggcag ttacagcctg accgttgacg acacggtgtt tgcgatggtt
    33421 tctgatggtg agttgtatct tcgggcttgt gagcaaagtg cacagtactg tgtaaaacat
    33481 ccgcctgtct ggctgacata taaaaagtgt ggccgatccg ttaccctcaa ttactatcgg
    33541 gttgatgaaa gtctatggcg aaatcaactg aagctggtgc gtctgtcgaa atattctctc
    33601 gatgctgcgc tgaaagagaa aagcacgcgc aatacccggg aaagactgaa agatttgccc
    33661 aatatgtctt ttcatctgga agcgattctc ggggaggtgg ggattaagga tgtacgggcg
    33721 ttacgtatac ttggggcaaa aatgtgttgg ttgcgactgc ggcagcaaaa cagtctggtg
    33781 acagaaaaga ttctgtttat gcttgaaggg gccattatcg gcattcatga agctgcgctc
    33841 ccggtggcac gccgccagga gcttgcagaa tgggctgact ctcttacgcc gaaacaggag
    33901 tttcctgcgg aacttgagta atctggcgtt gcagacgacc aatctctggc agtaatgcaa
    33961 tcaataaccc aacttgttgt acgaccagag gttctttgct gtctgcccgt ggttcaagtt
    34021 gctgcatccg ctgtttcagg ccagctaatg cctgattgac gcgttcttca tcagcaggtt
    34081 gatgatgtaa cgcgtcatca acatagcaaa ctgcgtcatc aagaaacgcc agaatttcag
    34141 gattagttaa ctgctcccgg tgagcaccga gggctgagat atagctggta aacgtatggt
    34201 taaggcacag caaccgaaac gcggcttcgc gaatttgcgg ggtaacgttc ggttcgctgg
    34261 acatatttga taccaccgac gccagttcag catcacggtt gtgtgcatcg cggcgggcaa
    34321 tacgatacgc cagacggtta tcacgcccct gatggtattg ctccagtatg gcatcgagat
    34381 accggcagtt ggcctcggtg gcgcgttcga gcattcgcgg cagattgcga aacttccagt
    34441 caggccagat gtagctcact gccgcccacg caatggcaca accaatcagc gtatcgatta
    34501 cgcgaggtaa cgccacttca aaaccttcac ccagcaagtt gaaacacagt agcaccaaaa
    34561 gtgtgatgaa catcgttgca tgggcgtatt gcacgttacg gaaggcaaaa aagagcacgc
    34621 cggtaataac cagcagcacc agctgccctt ccagtgatgg cacaaaccac agcacaggaa
    34681 taccaatcgc gatacctacc agcgtaccaa taatccttaa cttcaggcgg tggcgcgtgg
    34741 cgttatagtt tggctggcag acaaacaaac ttgtcagcaa gatccaatac ccgtgatgca
    34801 ttccggttat ctgaatgatg gcgtagccga agcacaacac cagcgacatt cttaccgcat
    34861 gacggaagag ggcggattcc ggcgtgaagt gacggctaag acgcagccag atatcactca
    34921 atccgtgcgg gctgtcatca gcgagctcat tttcgtcatt attatggggc agtgcctggg
    34981 cctgttctga ttcaattgtt gccagttggg catcaatggc acgtaaattg ttcagtaaaa
    35041 atcccagtgt tttgagtaaa tcagcaggag cgccgttatc gcgcatccgc tccagcgcag
    35101 catcaatatg cgtaaaagcg cgctcaaaat gcggatcatg ttgataaggc tgacgcagca
    35161 aaatacagcg tgacagttgc tggcacgcct ggccctgcat cgacatcagc cgctgaaaac
    35221 ggaacagcac gtcgctgtgg cgaaaatgtt cacgcaatgt ttgatattga atatgagaag
    35281 agctggcacg ctcgtgaata tcctgtgcga caaagtaata atgcagtgtg cgacgcgttc
    35341 ctcgttgacc acgatcgcca cgtaagcggg tcagcagcga gagtttcgtc tgattcaatg
    35401 tcgccatcag ctgaccgttg gcgagagcca aatcgtacag cggtgcctgg ctttcatctt
    35461 caatatcagg atcaaacatg cgcgacttga gctcaagata acgcgccagt tgttcatagc
    35521 aacgcgccag gttgtcctgc agcgggcgga ccgggaacag cagatgacca ataagtgtca
    35581 ggacgttgta ccagacggca ccggccagaa gatacatcgg ctgctgatac cagtgctcat
    35641 acagtgatgt tcccaacata gtgtaaatgg cgatcagcaa tgcaccgaag gcaattgttg
    35701 catagcgttg acccagaccg ccgagcaaaa tgaagccgct ggtagagagc gttaagccaa
    35761 tcgcaaatag ccagggccag ggaaacagca attctactga ggccgaggcg ataaaaaagc
    35821 agaacagcgt aatgatgagg ttacgcaaac gtcccgccag tcggtcatcg agatcggtca
    35881 gcgccgctgc caccatccca agcgtcagcg gaatcgtcag ttttacatca cccagccacc
    35941 acggaaacgc tgtggttcca caaagcgcaa taaaaatacg cgcgtaatac agccaggcgc
    36001 tgttccaggt atagcgtttg agcaaaggac ttagcataaa ggccatatta gtggattatt
    36061 ggaaacgacg acgtgcattg gcttcgcgcg cagcttgcgc tgtttctacc gataccacgc
    36121 gacgaccgac cggccatagt gcaatagcgg caattttaaa gttcgcaata ccgacaggaa
    36181 tgccaatgat ggaaatacat tgtgcgatgc ccgttgcaat gtgcatcagg cataaccacc
    36241 agccaaagaa aatcagccag aaaatattca ataccgtacc gccagtattt agcagcacat
    36301 ttttgccagc cgggttcagt tcatcgacat gaatagcttc attgccataa ggcaccagag
    36361 acagtttggt gatctcccag caggatcgtg tcagtggtaa ggtaaaaatc agcacaatac
    36421 tgaccagagt cgccaacagc cagcccagag tggtggcaaa tccgccaagc acaaagttca
    36481 gaatgttcaa aacggtacgc ataaaacctc gctttactgt ggttttcagt aatgggcggc
    36541 aattgtaacg tttttttggg ctggagcacg ttttctctga cggttacact gataagaaat
    36601 aatttcgtgt ggatctacag agtcatggaa ctgaaagcga caacgcttgg aaaacgtctg
    36661 gcacagcacc cttacgatcg ggcggtgatc ctcaatgccg ggattaaagt ctccggcgat
    36721 cgccacgaat accttattcc tttcaatcaa ttactggcga ttcactgtaa gcgcggtctg
    36781 gtatggggcg aactggagtt tgtactgccg gacgaaaaag tggtgcgtct gcacggcacc
    36841 gaatggggcg agacgcagcg tttttaccat catcttgatg ctcactggcg gcggtggagt
    36901 agcgagatga gcgaaattgc atctggtgtt ttacagcagc aactggattt aattgccacg
    36961 cgcaccggag aaaataaatg gctgacgcgt gagcaaacct ctggcgtgca gcaacaaatc
    37021 cgccaggctt tgtcggcgtt gccgttgccg gttaaccgac tggaagaatt cgataactgc
    37081 cgtgaggcgt ggcgtaaatg tcaggcctgg ttgaaagata ttgaaagcgc tcggttgcag
    37141 cataatcagg cgtataccga agccatgctt accgagtatg cggatttttt ccgccaggtc
    37201 gagtcttcac cgctgaatcc ggcgcaggcc cgggcagtcg ttaatggcga gcattctctg
    37261 ttagtgctgg ctggtgcagg aagcggaaaa acgtcggtgc tggtggcccg tgcaggctgg
    37321 ttgctggcgc gtggtgaagc gtcccctgag caaattttat tgctggcgtt tggtcgcaaa
    37381 gccgctgaag agatggacga gcgtattcga gaacgactac ataccgaaga cattaccgca
    37441 cgcacatttc atgcgctggc gctgcatatt attcagcagg gcagcaaaaa agttccgata
    37501 gtcagcaaac tggaaaatga taccgctgcc cgtcatgaac tttttattgc tgagtggcgc
    37561 aagcaatgca gcgaaaagaa agcgcaggcg aagggctggc ggcaatggct gacggaagaa
    37621 atgcagtggt cagtgccaga aggtaatttc tgggatgatg aaaaattaca gcgtcgtctt
    37681 gcctctcgcc tcgatcgctg ggtaagtctg atgcggatgc acggtggtgc acaggcagaa
    37741 atgattgcca gtgcacccga agtgattcgc gatctgttca gtaaacgtat caagttgatg
    37801 gctccgttat taaaagcctg gaaaggtgcg ctgaaggcag aaaacgctgt cgatttttcg
    37861 ggccttattc accaggcgat tgtgattctg gagaaaggtc gctttatcag cccgtggaag
    37921 catattctgg ttgatgaatt tcaggatatc tcgccgcagc gtgcagcgtt gttagcggca
    37981 ttacgcaagc aaaacagtca gacgacgttg ttcgccgttg gtgatgactg gcaggcgatt
    38041 tatcgcttca gcggtgcgca aatgtcgctc accaccgctt tccatgaaaa ctttggtgaa
    38101 ggtgatcgct gcgatttaga cacgacttac cgttttaaca gtcgtatcgg tgaggtggca
    38161 aaccggttta ttcagcagaa cccaggccag ctgaaaaagc cgctaaacag cttaaccaat
    38221 ggagacaaaa aagccgtcac gttattggat gagagccatc ttgacgcttt gctggataag
    38281 ctctctggtt atgccaaacc ggaagagcgc attctgatcc tggcgcgtta ccatcacatg
    38341 aggcctgcca gcctggaaaa agcggcaaca cgctggccga agttgcaaat cgactttatg
    38401 accattcatg ccagcaaagg gcaacaggcg gattacgtca tcatcgttgg cttgcaggag
    38461 ggaagtgatg gttttccggc tgcggcgcgg gagtcgatta tggaagaggc gctactgcca
    38521 tcggttgagg atttcccgga cgctgaagaa cggcggttaa tgtacgtggc gctgacccgg
    38581 gcacgccatc gggtatgggc actgtttaac aaagagaatc cctctccatt tgtcgaaata
    38641 ctgaaaaatc ttgatgtgcc ggtggcgaga aaaccgtaag aaacaggtgg cgtttgccac
    38701 ctgtgcaata ttacttcaga cggtccgcga gataacgctg ataatcgggg atcagaatat
    38761 cgaccgcgtc gttaaaatgc ggtgactgga taatgaagtc tgccgttgcc acgttggtgg
    38821 cgaccggaat gttccatacc gttgccagac gcagcaaggc tttcacgtca ggatcgtgcg
    38881 gcacggcgtt tagtgggtcc cagaagaaaa tcaatacatc aattttccct tctgagatca
    38941 atgcgccaac ctgctggtca ccccccattg ggccactcaa catcgcgttg acgttcatgc
    39001 cggtcgcgcg ggaaattaag ttaccggtag tgcctgttgc atacagtacg tgttgttcca
    39061 gtaacggttg atgccgttcc acccagctca tcagcatttg tttgcagtga tcgtgtgcca
    39121 ccagcgcaat atgtttccgc gcaggtaaag tgcgtgtcgt cagttccata atgtacatcc
    39181 gtagttaact ttcctacaga ttactgtaag cacttatcgc tgcaagataa agaccgaaaa
    39241 agcctgcgca caggcacaaa aatctcagga agatggttgt ttttccgccc attgcaggaa
    39301 agtatttcgc gtttgtgggt cagccagttt aaaccaatac ttcagccgtt gttctgtgag
    39361 cacctgagac tgcggtggaa tagcatccag ctcggaaaca cccgaaagca gcgtactatc
    39421 atctgccatc atcgtggcaa attgcggtag tgaagcgcgt ttcgcggatt tgttatagcg
    39481 ttcaacctcg acctcataat caatcgtttt tgccgttgag gtaatggcga gaatatccag
    39541 ctttaccgga atcggcgtcg catcgccatc caacaattca aggcgcggcg cggcatcaaa
    39601 atggttagct tcccgctcat tctccagacg aggcaggcga aaattgacct ggttgatgag
    39661 ctgggtatta aaactgacca ccagcggtgg ggagatatac agccgttctt cactattgga
    39721 aagatgaatc gtcttctcaa cgcgaaacac taactgatgc ggtccgttat ccagttcaat
    39781 gctgtccgca ccgcgcagca gagagctgga aaccttcttg ccgtcgagca ccagaagatc
    39841 gacatcggtt gaaagccgca gcgtggtggc aaaaacggat accggcaaac ataatgcaat
    39901 caaggtggtc acgatgccgg ttttcatagg tagctcctgt caaaagaccg cccgaataat
    39961 aatgtgtcaa aatttttctt taatcatact tactaacata tagacatatt tcccgctttt
    40021 gctctcattc attcgtatta gctgcatggt tggcatgtcg gctttgtcgt acactttgtt
    40081 aaacagtctg gaggaaataa tgaaagaaac cgatattgca ggcattttaa cgtccactca
    40141 caccattgcg ctggtggggg caagcgacaa acccgatcgt cccagctatc gcgtgatgaa
    40201 atatctactc gaccaggggt atcacgtcat tccggtttcg ccaaaagttg ccggcaaaac
    40261 actgctgggc cagcaggggt atggcacgct ggcagaagtt cctgaaaaag tcgatatggt
    40321 cgatgttttc cggaattcag aagcagcgtg gggcgtagcg caggaagcga tcgccatcgg
    40381 cgcgaaaacc ctctggatgc aactgggggt gattaatgag caagcggcag tactggctcg
    40441 ggatgccgga ttaaatgtag tgatggatcg ctgcccggct attgagatcc ctcgcctggg
    40501 cctggccaaa taaaaaatcc ccggaaggca aaaaccttcc ggggatttgt tcagggatta
    40561 gttacgcaga cgcggggcct ggagttgttt gcggatggtc tgcgccagct catccatgga
    40621 tggctgttca ggatgttcat cctgcaattc gctacttagc tgggcttcag ccaggtaggt
    40681 atgaaccggt aggccgttat cgtcctccat caccacgtga taccacggag cggcgcgaag
    40741 ctcgtcattc accgccagct catcaggcga cggttcagag agcgaataaa ccgggtcgat
    40801 atccacgacc actccgagat aacctaacag ggaatggcgg acctgctggc cgataccgaa
    40861 tttgctggca atcatagtca cctcccggga aatctttacc tttgatataa gggtaaaatt
    40921 ccacatttca agttacatga cgcgacaggc aaaccctttc agatatagcc cttccgggta
    40981 ggtagcgatc accggatgat cggctgcctg acggaactgc tctataaatt gtacatcacg
    41041 accggcatca attgcggcat ccgcgatgat tttctgaaat aaatcgctgg tcatcagacc
    41101 ggaacaggag aaagtcagga gaataccgcc ttcattcagc agctgaatcg ccagcatgtt
    41161 gatgtcttta taaccacgac acgcgcccat caactggctt ttattctcaa caaacttcgg
    41221 cgggtccatc acgataacgt caaatttttc accgcgatcg cgataagtac gcagcaattt
    41281 aaagacatca tcacggacaa actcagcctt gctcagatcc agtttgttca gctcaacgtt
    41341 ctgccgtgca atatccaggg cttcctggga ggtatcaacg ctgacaacct ggctgcaacc
    41401 gcccatcagt gccgatacgg cgaaaccacc ggtataggag aaacagttca gtacgcgttt
    41461 attttcaacg tagcggcggg tagccaggcg gctatcacgc tgatccaggt agtagcccgt
    41521 tttgtgtccg tgctgaatat ccaccagcag cttcatgccg tgttcttcaa tcggcagcag
    41581 ggcaggcggc aactcgccgg tgatggggcc ctgagtcagc tccatccctt cttttttacg
    41641 taccgcgacg tcgctgcgat cgtaaatagc acattccggg tacagcgttt gcagggcact
    41701 aattaatgcc gcgcgctgat actctgcgcc agcactcagc agttgcagca ccagaaaatt
    41761 accgaaacga tcgatagtaa tacccggcag gccatcagat tctccggcga ttaaacgata
    41821 gctgtcgagg ccatcttttt gcgccagcca gtcacgccat ttttgtgctt gctgtaaacg
    41881 gcgggtaaaa aaagcaatgt cgatagattc attcgggtca aacgtccaga cgcgcgcccg
    41941 gatttgcgaa gctggcgaat aagcgccgcg tgctaaccat tttccctgat gatcaacaat
    42001 atcgatggtt tcaccgaggc tggctttacc ttccatgcgg gcaacggccc cggaaaagac
    42061 ccacggatgg cgacgaagta atgatttttc gcgccctttg gctaacacta aacgtacact
    42121 cataatttac ttttctgtcg atgcaaagaa atgggcgtca ttgtccggag ttttccatgg
    42181 atttgcaacg cgtccattaa ggataaggag agcagcaatg tcgaaagtct gcataattgc
    42241 ctgggtttac gggcgagttc agggcgtagg atttcgctat accacgcagt acgaagcgaa
    42301 aaaactcggg ttaagcggct acgccaaaaa tcttgacgat ggcagcgtgg aagtggttgc
    42361 ctgcggtgaa gaagggcagg tggaaaaatt aatgcagtgg ttaaaaagtg gcggcccgcg
    42421 ttcagcgcgt gtggaacggg tgcttagcga gccgcatcat ccctccgggg agttaacgga
    42481 ttttcgtatc cgttaaatgc attttaccgg tttaggcagg ccagcaattt tggtggcttg
    42541 ctttgccgga cctttcggga acagtcggta cagatagcgg ctattgcctt tctcttcgcc
    42601 aaatttattc gccatcgctt ttaccagcat acgaatcgcc ggagaagtat tgaattccag
    42661 atagaaatca cgcacaaaac gcaccacttc ccagtgttct ggcgacagcg caatcccttc
    42721 gttctctgca atcaccaccg ccagtggctc actccactgg ctgctttctt tgagatagcc
    42781 ttcggtatcc gtttctatct ctttaccttc gaagatcagc ataattcttt actaccttaa
    42841 tcagactggc ggcagtgtaa caaaaaacaa agccccgcat aaagcgaggc tatgaaagtg
    42901 ttagcgggtg agattaatcg cggctggcga agcccagaat gctcagcagg ctgacgaaga
    42961 tgttgtacag cgaaacatac aggctaaccg tggcacgaat atagttcgtt tcaccgccat
    43021 gaatgatgtt gctggtttca aacaaaatag cgccagagga gatcagaatg aagaccgcgc
    43081 tgatcgccag atgcagagca ggcagttgca ggaagatatt agcaaccata ccaatcagca
    43141 ccaccacaat acccgccatc agcataccgc caaggaacga catatctttg cgggtggtca
    43201 gcacatacgc agagcagcag aagaacacta acgccgttcc gcccagtgcc atagcgatta
    43261 cgtcacccat tccggcagac aggtaggtgt tcagaattgg tccgaggata tatcccagaa
    43321 aaccggtaaa ggcgaatgcg gagataatcc cggtcggctt attcgccgtt ttataggtca
    43381 ggaacatcaa accatacata cccaccagcg tcagaatcag acccggagat ggcagcatca
    43441 gcaccgtact ggcagttgcg gtaatcgccg aaaaggccag cgtcaggctc agcagaaaat
    43501 aggtattacg cagcacctta tgggtgctaa gcagtgatgt acggtcatgt gaagaactaa
    43561 caatacgatc cattgagtca ctctctatga cagatgtaat taattaagca gcataatgat
    43621 aatgcgtaag ggcacccaga agttttaccc atctttacgc atttgatctg gaacaggttt
    43681 aacagcggat tatcaggtca ttaagcaaat ataacgctct gagaatttcg acagacaaaa
    43741 gaaaaagggg ttagcattta gctaacccct tatcttattt ggcggaagcg cagagattcg
    43801 aactctggaa ccctttcggg tcgccggttt tcaagaccgg tgccttcaac cgctcggcca
    43861 cacttccgga atgacgcgca ctataaacat cccgatgcgg cgtgtaaacc cctaatttgt
    43921 ttgtttgcct gaaaaacagc caaaagtgca ttgttagcgt gaaataacag cagattgctc
    43981 atttcatcac catgaattcc ttctctttta ctcgtttagc aaccggctaa acatccccac
    44041 tgcccggcaa aaagaaaaat aggtccattt ttatcgctaa aagataaatc cacacagttt
    44101 gtattgtttt gtgcaaaagt ttcactacgc tttattaaca ttactttctg gcgacgtgcg
    44161 ccagtgcaga aggatgagct ttcgttttca gcatctcacg tgaagcgatg gtttgccttg
    44221 ctacagggac gtcgcttgcc gaccataagc gcccggtgtc ctgccggtgt cgcaaggaga
    44281 agagacgtgc gatatgaata acgaggaaac attttaccag gccatgcggc gtcagggcgt
    44341 tacccggcgc agctttctca aatattgtag tctggctgcc acgtcgctgg gattaggcgc
    44401 gggaatggca ccaaagattg cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg
    44461 gatccacggt ctggaatgca cctgctgtac cgaatctttt atccgctccg ctcacccact
    44521 ggcgaaggac gtcatccttt ccctgatttc cctcgattat gacgatactt tgatggctgc
    44581 cgccggaacc caggcggaag aagtctttga agacatcatc acgcaataca atggcaaata
    44641 tatcctcgca gtagaaggta atccgccgct gggcgagcag gggatgttct gtatcagcag
    44701 cggacgaccg tttattgaga aactcaaacg tgccgctgcc ggagccagtg cgattatcgc
    44761 ctggggaacc tgcgcgtcct ggggctgcgt gcaggccgcg cgacccaatc cgacgcaggc
    44821 aacgcctatc gacaaagtca tcaccgacaa acctattatc aaagtacctg gctgcccgcc
    44881 gatcccggat gtgatgagcg ccatcattac ttacatggtg acctttgatc gtctgccaga
    44941 tgtcgacaga atgggccgtc cgttgatgtt ctatggtcag cgtatccacg ataaatgcta
    45001 tcgccgcgct cacttcgacg ccggagagtt cgtccagagt tgggatgatg acgctgcccg
    45061 caaaggttac tgcctgtaca aaatgggctg caaagggcct accacctata acgcctgttc
    45121 ctccacacgc tggaacgatg gcgtttcctt cccgatccag tcaggtcacg gctgcctggg
    45181 ttgtgcggaa aatggtttct gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc
    45241 gcaaatgggt acccattcca ccgccgatac cgtcggttta accgcacttg gcgtggtggc
    45301 agcggctgtt ggtgtgcacg cagtcgccag cgccgttgac cagcgcagac gccataacca
    45361 gcaacctaca gaaaccgaac atcagccagg caatgaggat aaacaggcat gagcactcag
    45421 tacgaaactc agggatacac catcaataat gccggacgcc gcctggtggt cgacccgatt
    45481 acgcgcattg aaggccacat gcgctgcgaa gtgaatatta acgatcagaa tgtgatcacc
    45541 aatgccgtct cctgcggcac catgtttcgc gggctggaga tcatcctgca agggcgcgac
    45601 ccgcgcgatg cgtgggcgtt cgttgaacgt atctgcggcg tctgtactgg cgtacacgct
    45661 ctggcttcgg tttacgccat cgaagatgcc atcggtatta aagtgccgga caacgctaat
    45721 atcatccgca acattatgct ggcaacgctc tggtgccacg atcatctggt gcacttctat
    45781 cagcttgccg ggatggactg gatcgatgtg ttagatgcgc tgaaagccga tccgcggaaa
    45841 acctccgaac tggcgcaaag tctctcctct tggccgaaat catcccctgg ctatttcttc
    45901 gacgtacaaa accgcctgaa gaaattcgtt gaaggtgggc agttggggat cttccgcaat
    45961 ggctactggg ggcacccgca gtacaaactg ccgccagaag ccaacctgat gggctttgcc
    46021 cactatcttg aagctctcga tttccaacgt gaaattgtca aaatccacgc ggtctttggc
    46081 ggtaaaaacc cgcatccaaa ctggattgtc ggcgggatgc cttgtgccat taacattgac
    46141 gaaagcggcg cggtcggggc ggtcaatatg gaacgcctga acctggtgca gtcaatcatt
    46201 acccgtacgg cggatttcat taacaacgtg atgatccccg acgccttagc catcggtcag
    46261 ttcaacaagc cgtggagcga aatcggcact ggcctttcgg ataaatgcgt tctcagctac
    46321 ggcgcattcc cggatattgc caacgacttt ggcgagaaaa gtctgctgat gcctggcggc
    46381 gcggtgatta acggcgactt caaaaatgtg ctgccagtgg atttagttga tccgcagcag
    46441 gtgcaggagt ttgttgacca tgcctggtat cgttatccca acgatcaggt tgggcgtcat
    46501 ccgttcgatg gcattaccga cccgtggtac aaccccggcg atgtcaaagg cagcgatacc
    46561 aacattcagc agctgaatga acaggaacgc tactcgtgga tcaaagcgcc acgttggcgc
    46621 ggtaatgcga tggaagtggg gccgctggca cgcacgttaa tcgcttatca caaaggcgat
    46681 gctgcgaccg ttgagtcggt cgatcgcatg atgtcggcgt tgaacctgcc gctttccggc
    46741 atccagtcaa cgttaggccg cattttgtgt cgcgcgcacg aagcgcagtg ggccgcaggc
    46801 aagttgcaat atttcttcga caagctgatg accaatctca aaaacggcaa tctcgccacc
    46861 gcctccactg agaagtggga accagcaacc tggccgacag agtgccgtgg tgtcggcttt
    46921 accgaagcgc cgcgcggagc gttaggccac tgggccgcca ttcgcgatgg caagattgat
    46981 ctctaccagt gcgtggtgcc gaccacctgg aacgccagcc cgcgcgatcc taaagggcaa
    47041 attggcgctt atgaagcggc gctgatgaac accaaaatgg cgatccccga gcaaccgctg
    47101 gagatcctgc gtactctgca cagctttgat ccgtgcctcg cctgttcaac acacgtgctg
    47161 ggcgacgacg gtagcgagct tatctccgtg caggtgcgtt aacagcgaag gagaatcatc
    47221 atgcaacaga aaagcgacaa cgttgtcagc cactatgtct ttgaagcgcc agtgcgtatc
    47281 tggcactggc tgacggtgtt atgcatggcg gtgttgatgg tcaccggata ctttatcggc
    47341 aagccgctac cttccgtcag cggcgaggcg acgtatctgt tttacatggg ctatatcagg
    47401 ttgattcact tcagcgccgg gatgattttt accgtggtct tgctgatgcg gatctactgg
    47461 gcttttgtcg gcaatcgcta ctcacgcgag cttttcatcg tgccagtatg gcgtaaaagc
    47521 tggtggcagg gcgtgtggta tgaagtccgc tggtatctgt ttctggcaaa acgtccgagt
    47581 gccgatattg gccataaccc catcgcccag gcggcgatgt tcggctattt cctgatgtca
    47641 gtttttatga tcatcaccgg ctttgcgcta tacagcgaac acagccagta cgctattttt
    47701 gcgccgttcc gttatgtggt ggaatttttc tactggacgg gcggcaactc aatggacatt
    47761 cacagctggc atcggctggg gatgtggctg attggcgcat ttgtgatcgg ccatgtctac
    47821 atggcgctgc gtgaagacat catgtccgac gacacggtga tctccaccat ggtcaacggc
    47881 taccgtagcc acaaatttgg caaaataagt aacaaggagc gttcatgagc gagcaactcg
    47941 tggtggtaat ggggctgggc aacctgctgt gggccgatga aggcttcggc gtgcgggtgg
    48001 cggaacggtt gtatgcccat taccactggc ccgagtatgt ggagattgtc gatggcggta
    48061 ctcagggact gaacttgctg gggtatgtcg aaagcgccag tcatctgttg attctcgatg
    48121 ccattgacta cgggctggaa cctggaacgc tgcgaacata tgccggagaa cgcattccgg
    48181 cttatctcag cgcgaagaaa atgagcctgc atcagaacag tttctccgaa gtgctggcgc
    48241 tggcagatat ccgcggacat ctgccagcac atattgccct cgtcggtctg caacccgcaa
    48301 tgctcgacga ctacggcggc agcctgagcg aactggcgcg ggagcaactg cccgctgcgg
    48361 aacaggcggc gctggcgcag ctggctgcgt ggggaattgt gccgcaaccg gctaatgaat
    48421 cgcgctgtct caattatgac tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgtc
    48481 agtacaggat gaggctggag gaacagggat gagcaacgac acaccattta atgcgttgtg
    48541 gcaacgaatg ctggcgcgcg gctggacgcc ggtcagtgaa tgccgtcttg acgactggct
    48601 tacgcaagcg ccagacggcg tggtgttatt aagtagtgac ccgaaacgca cgccagaagt
    48661 cagcgataac ccggtaatga ttggcgaatt actgcgcgag tttcccgact atacatggca
    48721 ggtagcgatt gctgatcttg agcagagcga agccatcggt gatcgttttg gcgtatttcg
    48781 ttttcccgcc acactggtgt ttaccggtgg tagctatcgc ggcgtgctga atggtattca
    48841 cccgtgggcg gaactgataa acctgatgcg tgggcttgtc gaaccacaac aggagcgtgc
    48901 ctcatgagcg aaactttttt ccatctgttg gggccaggaa cgcaaccgaa cgatgacagt
    48961 ttcagcatga atccactgcc gatcacctgt caggtgaatg atgaaccgag tatggcggcc
    49021 ctggagcaat gtgctcacag cccgcaggtg attgcgctgt taaacgagtt acaacaacaa
    49081 ctaagcgaac gccagccacc gttgggcgag gtgctggcag tcgatctgtt aaatctcaac
    49141 gccgacgatc gtcactttat caatacgctt ctcggggaag gggaagtgtc agtgcgcatt
    49201 cagcaggctg atggtagtga aagtgaaata caggaggcga tcttctgcgg attatggcgg
    49261 gtgcgcagac gtcgcggcga caagttgctg gaggacaaac tggaggctgg ctgcgcgccg
    49321 ctggcattgt ggcaggcggc aacgcaaaac gtcttgccga cagattcgct gttaccgccg
    49381 cccattgatg gcctgatgaa tggcctaccg ttggcgcatg agttactggc gcatgtacgt
    49441 aaccccgacg cgcagccgca cagcattaat ctgacgcaat tacccatcag cgaggctgat
    49501 cggctttttc tctcacgtct ctgtgggccg ggaaatattc agattcgcac cattggctat
    49561 ggcgagagct atatcaacgc cacggggtta cgccatgtct ggcatttacg ttgcacggac
    49621 accttaaaag gcccgttact ggaaagttat gaaatctgcc caataccgga agtggtgctg
    49681 gcagcgccag aagatttggt cgactcagcg cagcggctta gcgaggtatg tcagtggctg
    49741 gcggaagggg caccttcgta aaaagacggt aagtatcgct ttcaggctta tgaatatcgc
    49801 aatcggcgaa tacctctggt cgtagagttt caggataaag agggagatct accattaacg
    49861 ggttattttt ctctcttcgc ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg
    49921 ctggcagttt gctctgaccg cgctgtatca ctttttattt gtacccctta ccctggggct
    49981 gatttttttg ctggctatca tggaaaccat ttacgtggtc accggcaaaa caatctaccg
    50041 cgatatgacg cgcttctggg gtaagctctt cggtatcaac tttgctcttg gcgtggctac
    50101 cggcctgacc atggagtttc agtttggtac taactggtca ttctattcca actatgtggg
    50161 cgatattttt ggcgcaccgc tggcgatgga agcattaatg gccttcttcc tcgaatccac
    50221 ctttgtcggg ctgttcttct tcggctggca gcggctgaat aaataccagc acctgctggt
    50281 gacgtggctg gtggcgttcg gttcaaatct ctctgcattg tggatattga atgccaacgg
    50341 ttggatgcaa tacccgaccg gtgcgcattt tgatatcgac accctgcgta tggagatgac
    50401 cagtttcagc gagttggtct ttaacccggt cagccaggtg aaatttgtgc acaccgtaat
    50461 ggcgggctac gtgaccgggg ccatgtttat tatggcgatc agcgcctggt atttactgcg
    50521 cggacgggag cgcgatgttg cattacgctc gtttgccatc ggttccgtct tcggtactct
    50581 ggcgattatc ggcacgctgc aactcggaga cagttctgcg tatgaagtcg cgcaagtaca
    50641 accggtaaaa ctggcggcga tggaagggga gtggcaaacg gaacctgcac ctgcaccgtt
    50701 ccatgtggtt gcctggccgg aacaggatca agagcgtaac gcctttgcta tcaaaattcc
    50761 cgcgctgcta gggatcctcg ccactcactc attagataaa cccgtgccgg gtctgaagaa
    50821 tttgatggct gaaacctacc cacgcttgca acgcggacgt atggcctggc tgttaatgca
    50881 ggaaatatcg caaggcaatc gtgagccgca tgtgttgcag gcatttcggg agctggaagg
    50941 cgaccttggt tacggcatgt tgctctcccg ctatgcgccg gatatgaacc atgtcacagc
    51001 tgcacagtac caggcggcga tgcgtggcgc gatacctcag gttgcgccgg tattctggag
    51061 tttccgcatc atggtgggct gtggttctct gctgctactg gtgatgctga ttgcgcttgt
    51121 ccagacgctg cgtggcaaaa ttgaccagca tcgctgggtg ctgaaaatgg cgctctggag
    51181 tttgccgctg ccgtggattg cgattgaagc cgggtggttt atgaccgagt ttggtcgtca
    51241 gccgtgggcg atacaggaca tcttaccgac atactccgcg cactccgcct taaccacagg
    51301 acaactggct ttctcactga tcatgatcgt agggctttac accctgttct taatcgccga
    51361 agtctacctg atgcagaaat atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc
    51421 gacgcagcaa caggggtaaa ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc
    51481 tggtggctgc tgattggcgt gatcctggtg gtctttatga tctccgacgg atttgacatg
    51541 gggattggct gtttgctgcc gctggtggcg cgtaatgatg atgaacgtcg gatagtgata
    51601 aacagcgttg gcgcgcactg ggaaggcaat caggtctggt tgatcctcgc aggtggggca
    51661 ttatttgccg cctggcccag agtgtatgca gcggcgtttt ccggctttta tgtggcgatg
    51721 attctggtgc tgtgctcgct gttcttccgc ccgctggcct ttgattatcg cggaaaaatc
    51781 gccgatgcac gctggcgtaa aatgtgggat gccggactgg tcatcggcag tctggtgccg
    51841 ccggtagtct tcggtattgc cttcggcaac ttgctgctcg gcgtgccgtt tgctttcaca
    51901 ccgcaattac gcgtggaata tctcggcagc ttctggcaac tgctgacgcc attcccttta
    51961 ttgtgtggat tgctcagtct tgggatggtg attttgcaag gtggcgtctg gttacaactg
    52021 aaaactgttg gtgtgattca tctgcgttca cagctggcga ccaaacgcgc cgcactactg
    52081 gtgatgctgt gctttttgct ggcgggttac tggctgtggg ttggtattga tggctttgta
    52141 ctgctcgccc aggatgctaa cggtccttcc aatccgctaa tgaaactggt ggcagtgcta
    52201 cctggtgcct ggatgaataa tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg
    52261 gggttcttct gcccattgct gacggtgatg gcgatttatc gtggtcgccc gggttgggga
    52321 tttttgatgg cgtcattgat gcaatttggc gtgattttca cggcaggcat cacgctgttc
    52381 ccctttgtca tgccgtcaag cgtgagtccg atctccagcc tgacgttgtg ggacagcact
    52441 tccagtcagc tgacgctgag cattatgttg gtaatcgtgc tgatattttt gcccattgtg
    52501 ttgctctaca ctctctggag ctactacaaa atgtgggggc gcatgacgac agaaactctc
    52561 cgccgtaacg aaaacgagct gtactaagga gcaggaacaa tgtggtattt actttggttc
    52621 gtcggcattt tgttgatgtg ttcgctctcc acccttgtgt tggtatggct ggacccgcgt
    52681 ctgaaaagtt aacgaacgta agcctgatgt ggcgcattag catctcatca ggcaatcaat
    52741 aatgtcagat atgaaaagcg gaaacatatc gatgaaagcg atcttaatcc catttttatc
    52801 tcttctgatt ccgttaaccc cgcaatctgc attcgctcag agtgagccgg agctgaagct
    52861 ggaaagtgtg gtgattgtca gtcgtcatgg tgtgcgtgct ccaaccaagg ccacgcaact
    52921 gatgcaggat gtcaccccag acgcatggcc aacctggccg gtaaaactgg gttggctgac
    52981 accgcgcggt ggtgagctaa tcgcttatct tggacattac caacgccagc gtctggtggc
    53041 tgacggattg ctggcgaaaa agggctgccc gcagcctggt caggtcgcga ttattgctga
    53101 tgtcgacgag cgtacccgta aaacaggcga agccttcgcc gccgggctgg cacctgactg
    53161 tgcaataacc gtacataccc aggcagatac gtccagtccc gatccgttat ttaatcccct
    53221 aaaaactggc gtttgccaac tggataatgc gaacgtgact gacgcgatcc tcagcagggc
    53281 aggagggtca attgctgact ttaccgggca tcggcaaacg gcgtttcgcg aactggaaca
    53341 ggtgcttaat ttcccacaat caaacttgtg ccttaaccgt gagaaacagg acaaaagttg
    53401 ttcattaacg caggcattac catcggaact caaggtgagc gccgacaatg tctcattaac
    53461 cggtgcggta agcctcgcat caatgctgac ggagatattt ctcctgcaac aggcacaggg
    53521 aatgccggag ccggggtggg gaaggatcac cgattcacac cagtggaaca ccttgctaag
    53581 tttgcataac gcgcaatttt atttgctaca acgcacgcca gaggttgccc gcagccgcgc
    53641 caccccgtta ttagatttga tcaagacagc gttgacgccc catccaccgc aaaaacaggc
    53701 gtatggtgtg acattaccca cttcagtgct gtttatcgcc ggacacgata ctaatctggc
    53761 aaatctcggc ggcgcactgg agctcaactg gacgcttcca gctcagccgg ataacacgcc
    53821 gccagggggt gaactggtgt ttgaacgctg gcgtcggcta agcgataaca gccagtggat
    53881 tcaggtttcg ctggtcttcc agactttaca gcagatgcgt gataaaacgc cgctgtcatt
    53941 aaatacgccg cccggagagg tgaaactgac cctggcagga tgtgaagagc gaaatgcgca
    54001 gggcatgtgt tcgttggccg gttttacgca aatcgtgaat gaagcacgca taccggcgtg
    54061 cagtttgtaa tgcataaaaa aagagcattc agttacctga atgctctgaa gctgatgaca
    54121 aacgaagaac tgtctgatgc gtaggccgga aaaggcgttc acgccgcatc cggccacctt
    54181 accttcagtt ttactctttc tcggagtaac tataaccgta atagttatag ccgtaactgt
    54241 aagcggtgct ggcgcgttta atcacaccat tgaggattgc gcctttaata ttaacgccag
    54301 cctgttccag acgctgcatt gacaagctca cctctttggc ggtgttcagg ccaaaacgcg
    54361 caaccagcag gctggtgccg acagagcgcc ccacgaccgc agcatcgctc accgccagca
    54421 tcggcggcgt atcgacaatc accagatcgt aatggtcgtt cgcccattcc agtaattgac
    54481 gcatccgatc gcgcatcagc agttcagacg ggttaggtgg cacctgaccg cgagtaatca
    54541 catcaaagcc tcctttgcca aaatgctgga tcactttgtt gagctcatct ttccctgcca
    54601 gatattccga caagccatgt tcattactca cggtaaacag gttatgcgaa taaccacgac
    54661 gtaagtcggc atcaataaat aacacttttt gatcggactg ggcgatcacc gccgccagag
    54721 ttgaactgac gaacgtttta ccactgtctg gcgtcgcacc ggtgatcatc agaatgttat
    54781 tctctgtctc catcatagcg aagtgcagac tggttcgtag cgcacgtacg gcttccacag
    54841 cagaatctgc cgggttatcc accgccagga aggggatatt tttagtacga tggcgctgct
    54901 gattagaaaa taaatttttc ttacgcagac gggtacgctt atccagccac tcggacatcg
    54961 ggatagtggc ataaacgctg atgccgtgct cttccagttg ttctggtgct tctacaccac
    55021 gacgcaacat cgcacgcgcc agcacggcac ccaccgaaat aaacaggcca agaataaaac
    55081 caagcaccac attcaacgct tttttcggtt tcactggctg cggctgagtg actgccgggt
    55141 cgataatccg cacgttacca atcgcactgg atttcgaaat actcaactcc tgctggcggt
    55201 taagtaattg cagatatacc gcacggcccg cttctacgtc acggcttaaa cgcaatactt
    55261 cctgttgggt ggaaggcatt gccgataccc gcttattcag gcgtttgcgt tcttgctcca
    55321 gcgtctggcg tttttccagc agcgcacgat aagttgggtg atctttctta tacagctggg
    55381 agatctctgc ctcgcggaag gtcagctcat tgagttgatt atcaacgtta acaatctgct
    55441 caagaacggc tttggcttcc aggttaaggt caaccgaatc gcgctgctgg cgataaacgt
    55501 tgagtttttc ttccgcctgg tccagctcgc tgcgcacttc aggcaactgg cgctgtaaga
    55561 attcaaggct ttgtgaatcc tgcgccgcct ggcgagcgat attctgttgc aaatagttgt
    55621 tagcgatgct gttcagaata cgggtaatca actggggatc atcaccggtc atggtaagtt
    55681 ccagcatccc gctttcttta ctgcgttcgc taacggtaaa ggtttcctgc aacgcgttaa
    55741 tcgcttccag ttcggtacgc tggctcagga caaactgtgt tcctggtttg gccttaatgt
    55801 ccgcgatagt cagcgcaacg ccatcttttt ccagacgctg accgaccata ccattgacgg
    55861 tgaactcttc accttccagc gtatagtggc cgttttcccc aaccgtgagt gtcagttgct
    55921 gatcctgacc attcagttgt ggaatatgca tccagctgat cgccagctca ccaggtttct
    55981 ctttggttaa tctcgcccag ccgcgaccca caatcggaaa atacttctgc tcaaccatgt
    56041 cgcgcagatt cagttcagca atggttttac cgagaatcat gcgcgattgc agcagttgta
    56101 tctccggtgc agactcgggc gatgagttag ggatcatatc gctcaggccg ctgagaatgg
    56161 cgttgccctg tttctgctca acctggacca gagtatctgc ctgataaatt ggtgtgctta
    56221 acagcgagta agcgacagcg atcagcgtga ataacgcagt cacgctgaca ataaacttac
    56281 ggtgatccca taactcgccg accagacgaa gcagatcgat ctcattttcc tgagtgctgc
    56341 ctggtggcgt attcatattt ttagttgtca tcgtatttta taaactcaaa ttaccggctg
    56401 aggcgcttcg cccattcctg actggcgcgc tccaacatac cgtagacatg ttcaaatgcg
    56461 tcctgacttt tacgataggg atccgggatc tctttctgtt ccagccattg cccaaacagc
    56521 attgtcttcc cgcggacctc gggagcgatt gcggtaacct gagcaatatg ttccgactcc
    56581 attgccagaa tcagatcgta atttcgtgcc atctcagcag tgagcttacg tccggcatgt
    56641 ccttcaagag atacgccatg atttgcggca acatctgctg ctgtcgcatc tgcagggtgt
    56701 tttaccagac catgaacacc tgccgatttg acttttacgc ctggcagacg tttacgcagc
    56761 aagcgctcgc caatgggcga acggcaaatg ttgccggtac aaaccaccag gattgagtta
    56821 aattttagtt gggccatgtt tgaatccacc gtgaagtctc tgtcaggtcg tgcaccccgg
    56881 aaatcgtcgg taccagcaga gaaatcacac ggttccaacg agccagcggc gcggtagtga
    56941 cataaacaat gtcgtaaggt tcaagctgga attccgttcc gagtatcatt gccgatgcgt
    57001 ccttcgcatt cagctgataa atattggcga ttttgccatt ctgcttgttt tgcgttgcac
    57061 gaataacaaa aatccccgtc gcatcagcga catcctgatt cataccctca gcgttaccca
    57121 gcgcttccgc cagggtcata ccgctgcggt ccattttcag cgtgctctgt ttacccactt
    57181 cacccatcac gaagactttc agatcatcgt tgcgcggaat aaataaaata tcgcccgggt
    57241 gcagcagttt gttctggcgc aagtcgccac gctgcatcag cgcatagagg ttcactttgg
    57301 tctttacgcc attctgggtg agcaccacgt tgcgccagtc tgcatctgcg gtcagaccgc
    57361 ccgcggcgtt aatcgcatcc atgatggtca gaggaatatt ggtaatcggc tgctgacccg
    57421 atttgctgac ttcgccggtg acgtacgctt tttgcgatcg gaacgccgcg acgctgacat
    57481 cgacctgcgg gctttcaatg actgaatcca gacgcgctgt aatctcgttg cgtacctggg
    57541 taagcgtttt cccggccact ttcaggcggc caatataagg gtagaaaatt gcaccatcag
    57601 cgttaaccca gttgccggta tcactggcgc tacgatattg ccctgcgggt gtggttagtt
    57661 ccgggtgatc ccagaccgta accatcaaca catccccaat gccgatacga tattcatagt
    57721 tcgcgatttg ctgctccagc tccggattag cctgcgacat aatcggtttg gcgcgcagct
    57781 gatcaatcaa tcctggcgtg acgggataga tattgaccat tttgtccagg tcatactgat
    57841 tgtcaggtag ttcaatgaca tccttgttac tggtcgataa attctgacca ggaacaaggg
    57901 tacatgctgt caaagacaga acagccaatg tcagtaccga aaacttaaaa atgttctttt
    57961 tcattggttt atatttatca gtcaatgtaa tgttatgaat tacgtcaggt tattgtccaa
    58021 ccggtacttc tcgctctgcc gtcattggat agagctggta tttacgtgac agcatttgtc
    58081 caccatcacg cgtcaatggt gtccacgaaa ccaccgcgcg gttgcggtta ggtccaatgg
    58141 tcatcaagtc gaacggaatc gagatataaa aacctttact aaagccgcct tcgccgtagt
    58201 catcttttga tacgttactg attgccgccc atacccctac tgccacgccg ctgtcaaagc
    58261 gtttggcgac gtcgatcgtt gcacctttat cttttgccag atattgccca acgctaagtt
    58321 tcatcagtac gccattgagc gtcggcgggt tccagtaagc cgtcacgaaa cctgttggcg
    58381 tggaataatc ggtgaagcgc atcatgttgt cccagtcacg ctgcttcacg tagttaacgt
    58441 ccacacccag cgcccagctg gcatctagcg ggcgataaag cagctcggaa ccgacaccgg
    58501 catacatcgt ttccagataa ccgccataca cctggccata gaaaccgttg cctaagtcgg
    58561 caaagtagtt cgcttgcaag ttgttgagat agacgtcatt gcgaacgtaa tcacggatat
    58621 gcgtgcgcac gcggggcagg gtagagtccg cgggtaatag cgaagactta aacttgtcgt
    58681 agttattgta aatattggtg aaaataccgc catcaagcag caggtggtcg gtaaaccagt
    58741 agcgggcgct ggacatcagc cctaactgga acatatagaa atcttccggg ccgcccagcg
    58801 actgtgaaag tgttgggttg aaagagtagc taaagcgatc ttcacgaata cggtagcccc
    58861 gaccaaaggc agaaagatct tcagcttcaa cacgttgttg ttgcagtggc tctgattgac
    58921 caggcgctgt accggccagt tgtttgcgca ggctggctac gtcggtttcg gtagtcacca
    58981 tcgccatatg ctcgcgcttt tgcgtcacgc taatcttctc aacgccttgc ggcaggttat
    59041 tcaccagaat ccggttggca cgatcgaccg cttcgcgaga gtcacggtat ttgtattgct
    59101 gaccagacat atacagcgtc ttatcgcgta gctgaatttc tggcgcgtca aagcccgcgt
    59161 tatacttcag ggcggtaagt tggtttgcta ccgtggtgta ctgcaatcct tcagattccg
    59221 gcgcaggttg atatgccggt tttggcgtat cgcgcagggc agggcgcaga tcgttgaaat
    59281 tggtccgtaa cgtgaagcca aacatcaacg tgttaccgcg ttcataactc aggttgagat
    59341 ctgcccagct ggcagcgcga tagactgcgc cgacgttgaa atggcttgcc tgaggcagtt
    59401 tgccagcgaa atcattctgg taattgttgc catcgtattc gagtttcaga cgcagaggat
    59461 tccacggcgt ttgatactca ataccgccaa aaatagaggc tggaccacga aagatatcgc
    59521 taaagctgat atctcccgca tcgtgagact ctgcgcgatg gcaatattta tcgcttaccc
    59581 gacaaaacgg gttggtaata ttgcccgcat taccggcgta gccccaggcc atcccgaggg
    59641 tgaaatcgaa cgggcccgct tgcttgctgg cgaccagata ttcaccgtcg aacagacccg
    59701 taccagcaat atcacgttta ccaaatgcca cttgcggtag ccagtaacct tcttcccaca
    59761 ggcgaagttt aaaatcgaaa gatttgtctt tgtatgactg atcgccgctg aaatcttccc
    59821 actggctata tttgcgtgtg cgcacatccg tataacgaat ggtgccttcc agccatggga
    59881 acagcgccac ggaggtggag tagaaacggt attgatcgtt atcccggtag tttacgctga
    59941 attcaccttc cggtgcgatg cgcgcatttg gcatctgcaa caatcctgtg ccgccaaagt
    60001 ctgattgcga cgaacccagc ggatccggat aggttaatac ttcagcatga caggcactgg
    60061 agacggcaat ggccaggcag cttaaaagat aagagttctt cttcatcagt caggaacacg
    60121 ttgcgttaaa acagacacaa tctggtcatt taaatcagca tatttttcgg gcagtacatg
    60181 cgcagaaaag cccaaccaaa gctggctgcc aggcggtggt tccacatgtc ttttattcca
    60241 cagggcaaca ggggcgacga cagtctcacc ctcaggcgta atcaccataa cgttattctt
    60301 gtccgcgcct gcaaggcggg ggtgatcttg cagataatcg gtaacgctac ggcctgcctg
    60361 ccagggcaat tgcccagtcc cagaaaccgc acccagcaga gtaatagtca ccggacgctg
    60421 tacggtatag agcgtatagt ccccgaccag cgggggattg ctgttctcat ccactcgtac
    60481 aaaatcaggg tccagtttga ccggtaagcg accagtgatt ttgagattga gtaactgctg
    60541 gcgcacggat ttaatcgttg ccaccacatc gtcgtcagct tctgcttccc aggaagcaag
    60601 ctgtgccatg acatgttggt agtctttaag cgctttagct ttcgccgcag agtcggtcag
    60661 caaggcacca ggccaccaga ggcgatcgcg cagttgcggt tgtgtcacca actggacaac
    60721 attttccacg gggccaacgg aaagtgtctg ttgttcgcca ggcagataaa tagtcaccgt
    60781 tccttgcgca aaggcatggg gtgtcattac gtaaagtacg ctggcaatga aatacgactg
    60841 taatttattc atggtgcggg tttaagaaac gtcatttcta cgggaataac ccctgcgcct
    60901 aacatttgcc gactttgacg aacctggcca gtagccgaat cgacccagaa tgtgttttgc
    60961 cactgttttt ctggcagcgt ggaagtcacc tcttcctgcc agacattaca cagcacatcg
    61021 cgacccgcaa tattcagcgt ctctaatcca gcaaaggaaa aagtggaact cagggtcgca
    61081 gagcgaaaat ggttgtcttc actccagaga atatcgcgcg tccaacggct gccgtctttt
    61141 attgccagcg cgttacgcag agggtcctgc ccggaatttg tcacttccag cagattatta
    61201 ttaagtttga cggtctttaa aagacgtcca ttgtgggtaa ccagcatggc gttatcctgg
    61261 gataaccatt tgctttgttc ttgttctata tatcccagta ccacaaagat tcgttgccct
    61321 tcattaaggc gtaaatacat cgtggaataa ggcaacgcct ggatctgctg atccgctacg
    61381 gtgatatcct gattatcgaa aaggctggcg cgaaatgtat cgaccatact ttgctggctg
    61441 tgtgtacatc ccgcaagaaa tagtgcgaaa atcgataaaa taagagggcg cacctttaat
    61501 ccttgaaagg ttaattaaaa aaatgcaaat ctctaaagcc cggtacgaat atcgcaaccg
    61561 ggtatcattt aactttaatt tcgacgcgtt aaagcagtat tagcgtgtag tggacgtggt
    61621 ggttgaggtg ttatgagaac cgccatcgcc actgtcattt gctgcaacaa ctgccaacgt
    61681 cgccacacca acagccgttg ccacaccgac aaccgtcgcc gtgccgacag cgccagcagc
    61741 cacgccacca gtggtagcag cagtcgtggt tgcacctgta gtggtcgttg tggtgccagt
    61801 agtacccgtc gttgcctcgg tcccgtttgc tgcttcaggg gcagcaaatg cagctggcgt
    61861 cgtcaacatg aaggccataa ggattgcaga aagcttgtgt ttcataactt ttcctttatt
    61921 catcgcatgg acaatacgta cgcttgagat caggattatc tactttgagt ataacatcct
    61981 tgtgaaatct ccctttcctc ataccgccag cactatatac tgcgg
//
LOCUS       DAPHCV010000010        58743 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.010, whole genome shotgun sequence.
ACCESSION   DAPHCV010000010 DAPHCV010000000
VERSION     DAPHCV010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.010"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            188..859
                     /locus_tag="RH033_000648"
     CDS             188..859
                     /locus_tag="RH033_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDV2989882.1"
                     /translation="MDKVLDSAILSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIATVVMTTGNSGVDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     ENIIKNQIPSDVEIIRVYTMHDADRHQDHLAVYQASMVACRAIPQILGYETPSTWLSF
                     MPQVFESVKEEYFSLKLTALKKHKSQSQRDYMRPERLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            869..2065
                     /locus_tag="RH033_000649"
     CDS             869..2065
                     /locus_tag="RH033_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDV2989883.1"
                     /translation="MKTWIFICMAVAILLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFIAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWVGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSHPSVTGT
                     EKLSEA"
     gene            2100..2624
                     /locus_tag="RH033_000650"
     CDS             2100..2624
                     /locus_tag="RH033_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDV2989884.1"
                     /translation="MIIDESAGEVVIGANTRICHGAVIQGPVVIGANCLIGNYAFIRP
                     GTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVANQAYLGAQVRTSNHRLDEQ
                     PVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQLGPRVIVERNLPT
                     GTYSLRQELIRTGD"
     gene            2626..3399
                     /locus_tag="RH033_000651"
     CDS             2626..3399
                     /locus_tag="RH033_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HDV2989885.1"
                     /translation="MIKRTLLAAAIFSALPVYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEYFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGISLYTGPVISSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3584..3859
                     /gene="frmR"
                     /locus_tag="RH033_000652"
     CDS             3584..3859
                     /gene="frmR"
                     /locus_tag="RH033_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HDV2989886.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3894..5003
                     /gene="frmA"
                     /locus_tag="RH033_000653"
     CDS             3894..5003
                     /gene="frmA"
                     /locus_tag="RH033_000653"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HDV2989887.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5095..5928
                     /gene="fghA"
                     /locus_tag="RH033_000654"
     CDS             5095..5928
                     /gene="fghA"
                     /locus_tag="RH033_000654"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDV2989888.1"
                     /translation="MELIEKHASFGGWQNVYRHYSPSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIHNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFTAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6155..6694)
                     /locus_tag="RH033_000655"
     CDS             complement(6155..6694)
                     /locus_tag="RH033_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HDV2989889.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6796..8007)
                     /gene="mhpT"
                     /locus_tag="RH033_000656"
     CDS             complement(6796..8007)
                     /gene="mhpT"
                     /locus_tag="RH033_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HDV2989890.1"
                     /translation="MSTRTPSSSSTRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8183..9196)
                     /gene="dmpG"
                     /locus_tag="RH033_000657"
     CDS             complement(8183..9196)
                     /gene="dmpG"
                     /locus_tag="RH033_000657"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HDV2989891.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAVADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9193..10143)
                     /gene="mhpF"
                     /locus_tag="RH033_000658"
     CDS             complement(9193..10143)
                     /gene="mhpF"
                     /locus_tag="RH033_000658"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HDV2989892.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEENVAQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10140..10949)
                     /gene="mhpD"
                     /locus_tag="RH033_000659"
     CDS             complement(10140..10949)
                     /gene="mhpD"
                     /locus_tag="RH033_000659"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HDV2989893.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQHDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNETIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGNLS"
     gene            complement(10959..11825)
                     /gene="mhpC"
                     /locus_tag="RH033_000660"
     CDS             complement(10959..11825)
                     /gene="mhpC"
                     /locus_tag="RH033_000660"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HDV2989894.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVKAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11843..12787)
                     /gene="mhpB"
                     /locus_tag="RH033_000661"
     CDS             complement(11843..12787)
                     /gene="mhpB"
                     /locus_tag="RH033_000661"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HDV2989895.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12789..14453)
                     /locus_tag="RH033_000662"
     CDS             complement(12789..14453)
                     /locus_tag="RH033_000662"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HDV2989896.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFNQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQWKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            14530..15477
                     /gene="mhpR"
                     /locus_tag="RH033_000663"
     CDS             14530..15477
                     /gene="mhpR"
                     /locus_tag="RH033_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
                     /protein_id="HDV2989897.1"
                     /translation="MIFYCALSIGRVFSATIKTCPNVHQVHNVVLTIEMSINMQNNEQ
                     TAYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLHRTTVRRLLETLHEEGYVRRS
                     PSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLREVVWPTDVSTLDVDAMVVRE
                     TTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQERKELIEMLASRPGDDYQLAR
                     EPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLRSEQRVIGCLNLVYMASAMTI
                     EQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHLR"
     gene            15554..16636
                     /gene="lacI"
                     /locus_tag="RH033_000664"
     CDS             15554..16636
                     /gene="lacI"
                     /locus_tag="RH033_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HDV2989898.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQACHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQNAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVERKTTLPPNT
                     QTASPQALADSLMQLARQISRLESGQ"
     gene            16759..19833
                     /gene="lacZ"
                     /locus_tag="RH033_000665"
     CDS             16759..19833
                     /gene="lacZ"
                     /locus_tag="RH033_000665"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HDV2989899.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNIDECWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLSVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDELRVTVSLWQGETQVASGTTPFG
                     GEIIDERGGYADRVTLRLNVENPALWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPVLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSVKKWLSLPGELRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGRTIEVTSEYLFRHSDNELLHWTVALDGKPLASGEVPLDVAPQGKQVIE
                     LPVLPQPESTGQLWLTVHVVQPNATAWSEAGHISAWHQWRLAENLSVTLPAASHSIPH
                     LTTSETDFCIELGNKRWQFNRQSGFLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYLAEVALLQCTADILADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGHGEMVVSVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDLWDLPLSDMYTPYVFPSENGMRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            19885..21138
                     /gene="lacY"
                     /locus_tag="RH033_000666"
     CDS             19885..21138
                     /gene="lacY"
                     /locus_tag="RH033_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HDV2989900.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            21204..21815
                     /gene="lacA"
                     /locus_tag="RH033_000667"
     CDS             21204..21815
                     /gene="lacA"
                     /locus_tag="RH033_000667"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HDV2989901.1"
                     /translation="MNMPMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFASVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSVVIKDIPPNVVAAGVPCRVIREINDRDKHYYYKDYKVESSV"
     gene            complement(21918..23099)
                     /gene="cynX"
                     /locus_tag="RH033_000668"
     CDS             complement(21918..23099)
                     /gene="cynX"
                     /locus_tag="RH033_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HDV2989902.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQHRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQNAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LALQLVGFCGFIWLPLQLPVFWAMVCGLGLGGAFPLCLLLALDHSAQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(23105..23575)
                     /gene="cynS"
                     /locus_tag="RH033_000669"
     CDS             complement(23105..23575)
                     /gene="cynS"
                     /locus_tag="RH033_000669"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HDV2989903.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARQVGAKLDLDEDAILLLQMIPVRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(23606..24265)
                     /gene="cynT"
                     /locus_tag="RH033_000670"
     CDS             complement(23606..24265)
                     /gene="cynT"
                     /locus_tag="RH033_000670"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HDV2989904.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTSIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            24374..25273
                     /gene="cynR"
                     /locus_tag="RH033_000671"
     CDS             24374..25273
                     /gene="cynR"
                     /locus_tag="RH033_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414872.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HDV2989905.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDMLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAVHEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLQMALDKCAVVGGNESR"
     gene            complement(25406..26689)
                     /gene="codA"
                     /locus_tag="RH033_000672"
     CDS             complement(25406..26689)
                     /gene="codA"
                     /locus_tag="RH033_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HDV2989906.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(26679..27938)
                     /gene="codB"
                     /locus_tag="RH033_000673"
     CDS             complement(26679..27938)
                     /gene="codB"
                     /locus_tag="RH033_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDV2989907.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
     gene            complement(28265..30151)
                     /gene="prpE"
                     /locus_tag="RH033_000674"
     CDS             complement(28265..30151)
                     /gene="prpE"
                     /locus_tag="RH033_000674"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HDV2989908.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPGALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDIIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDREVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGNLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(30191..31642)
                     /gene="prpD"
                     /locus_tag="RH033_000675"
     CDS             complement(30191..31642)
                     /gene="prpD"
                     /locus_tag="RH033_000675"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HDV2989909.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMVKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            complement(31676..32845)
                     /gene="prpC"
                     /locus_tag="RH033_000676"
     CDS             complement(31676..32845)
                     /gene="prpC"
                     /locus_tag="RH033_000676"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HDV2989910.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEVQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            complement(33005..33895)
                     /gene="prpB"
                     /locus_tag="RH033_000677"
     CDS             complement(33005..33895)
                     /gene="prpB"
                     /locus_tag="RH033_000677"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HDV2989911.1"
                     /translation="MSLHSPGKAFRAALSKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRICAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAEAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARGQVK"
     gene            34134..35720
                     /gene="prpR"
                     /locus_tag="RH033_000678"
     CDS             34134..35720
                     /gene="prpR"
                     /locus_tag="RH033_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HDV2989912.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMQQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKMSLAALSVPFSAALRQGLETCQILLLLYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARKSAKTPIPGLLTAQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            complement(35821..36096)
                     /locus_tag="RH033_000679"
     CDS             complement(35821..36096)
                     /locus_tag="RH033_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV2989913.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            36243..36914
                     /locus_tag="RH033_000680"
     CDS             36243..36914
                     /locus_tag="RH033_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HDV2989914.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLHAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASWVIGAIIGVFALRLIYE
                     GVTQR"
     gene            complement(36931..37175)
                     /locus_tag="RH033_000681"
                     /pseudo
     CDS             complement(36931..37175)
                     /locus_tag="RH033_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(37797..38846)
                     /gene="yahK"
                     /locus_tag="RH033_000682"
     CDS             complement(37797..38846)
                     /gene="yahK"
                     /locus_tag="RH033_000682"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HDV2989915.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPRPHDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKYAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEHLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSESKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(38933..39889)
                     /locus_tag="RH033_000683"
     CDS             complement(38933..39889)
                     /locus_tag="RH033_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2989916.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGVNILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFTAVALGLWILLEKTPLGKSIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQARGMLGLDRVV"
     gene            complement(39886..40857)
                     /locus_tag="RH033_000684"
     CDS             complement(39886..40857)
                     /locus_tag="RH033_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2989917.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKMGGNFAVAFALAGGIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            complement(40850..42334)
                     /locus_tag="RH033_000685"
     CDS             complement(40850..42334)
                     /locus_tag="RH033_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDV2989918.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLSFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYQNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSEAKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            complement(42383..43366)
                     /locus_tag="RH033_000686"
     CDS             complement(42383..43366)
                     /locus_tag="RH033_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV2989919.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            complement(43620..44030)
                     /locus_tag="RH033_000687"
     CDS             complement(43620..44030)
                     /locus_tag="RH033_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDV2989920.1"
                     /translation="MTIKEKILQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTAELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            complement(44329..45711)
                     /locus_tag="RH033_000688"
     CDS             complement(44329..45711)
                     /locus_tag="RH033_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDV2989921.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGTSVPLAHAAVSGTIHCDAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTAHQTVEIQNGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQSVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPAGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELAHRMVAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(45721..46671)
                     /locus_tag="RH033_000689"
     CDS             complement(45721..46671)
                     /locus_tag="RH033_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HDV2989922.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGVFFSESQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFIVIGAGGGGIPVVRSEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLAFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(46703..46939)
                     /locus_tag="RH033_000690"
     CDS             complement(46703..46939)
                     /locus_tag="RH033_000690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2989923.1"
                     /translation="MRTLRRLTCKVRVVSAGCGVNALSGLRVAQESVGLISVAHQAFV
                     TIAGCGVNALSGLRMAQESVGLISVAHQAIWRWS"
     gene            complement(46918..48336)
                     /locus_tag="RH033_000691"
     CDS             complement(46918..48336)
                     /locus_tag="RH033_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HDV2989924.1"
                     /translation="MSPSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(48336..49883)
                     /gene="fdrA"
                     /locus_tag="RH033_000692"
     CDS             complement(48336..49883)
                     /gene="fdrA"
                     /locus_tag="RH033_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDV2989925.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKAGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVTVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETPVDEQGLQFARGSKEAALKAVMLSGMKQEHLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSVKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(49873..50736)
                     /locus_tag="RH033_000693"
     CDS             complement(49873..50736)
                     /locus_tag="RH033_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDV2989926.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDGLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMAAV
                     NFHLIAWQQWHDIIHQHLGENETLFNYCGDNPFYQALNKELHIKRRAVIQAVNDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALILFISGHPAEKYKEEFYLGLQRGRNNTTLLS
                     AITLEAAVQQRCRENIHRFIHNIIYDIPGHATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(50776..51381)
                     /locus_tag="RH033_000694"
     CDS             complement(50776..51381)
                     /locus_tag="RH033_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HDV2989927.1"
                     /translation="MTIKNLPADYLLAAQQGDIDKVKTCLALGVDINICDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDQICSNPFLISCLTGDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHMTDKYGKTPLELAQERGFEEIAQLLIAAGA"
     gene            51639..52136
                     /locus_tag="RH033_000695"
     CDS             51639..52136
                     /locus_tag="RH033_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDV2989928.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGLSTFWASVIMTVPFSAVV
                     VWQGRFGLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            52228..53160
                     /locus_tag="RH033_000696"
     CDS             52228..53160
                     /locus_tag="RH033_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2989929.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMAQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFSPNGILTGIYQDLLHREN"
     gene            complement(53202..54290)
                     /locus_tag="RH033_000697"
     CDS             complement(53202..54290)
                     /locus_tag="RH033_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDV2989930.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(54432..58415)
                     /gene="ehaA"
                     /locus_tag="RH033_000698"
                     /pseudo
     CDS             complement(54432..58415)
                     /gene="ehaA"
                     /locus_tag="RH033_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
BASE COUNT        13954 a        15965 c        14703 g        14121 t
ORIGIN      
        1 acacaaacac ttaacatatg aactacataa agattacaca gtcttatccc cgtacctggc
       61 agccccttgt ttttcagcta tcagctattt ttaacactta aaaattagca tctgtctgcg
      121 tcagaggtta cgtctctgcg ggcgtcgttg tgcttgtagt ataataaata aaatggagtg
      181 tcataaagtg gataaggttt tagattcagc gatcctttct tcggcaaata aaagaaaggg
      241 tatccttgcc attggcgcgc atcctgacga tatagaatta ggctgtggcg catcgctagc
      301 tcgccttgcg caaaaaggaa tttatattgc taccgtggtg atgactaccg gtaattctgg
      361 cgtagatgga ataatcgatc gccacgaaga atcacgcaac gctctaaaga tattagggtg
      421 ccaccaaact attcatctta attttgctga cacccgcgct catttacagc tcaatgatat
      481 gatttctgcc ctggaaaata tcataaaaaa tcaaattccc tctgatgttg aaattatacg
      541 tgtatatacc atgcatgatg ctgaccgcca tcaggatcat cttgcagtct atcaagcctc
      601 aatggttgcc tgtcgcgcta ttccgcaaat tctcggctat gaaacaccaa gtacctggct
      661 ttcatttatg cctcaggttt ttgaatccgt gaaagaagaa tatttttctc ttaaactgac
      721 tgcgttaaaa aaacataaaa gtcagagcca gcgagattat atgcgcccgg agcgtctgcg
      781 tgcagttgct caatttcgcg ggcaacaggt caatagcgat ctgggtgaag gttttgttat
      841 tcataaaatg attctttgag caaattgcat gaaaacctgg atctttatat gtatggccgt
      901 agcaatattg ctatggtttc tgagtacgtt aaggcgtaag cccagccaaa agaaaggctg
      961 tattgacgcc attatacctg cttataacga aggcccgtgt ctggcgcagt cactggataa
     1021 tctgctgcgt aacccttatt tctgccgggt aatttgcgtt aacgacggct ccacggacaa
     1081 taccgaagcg gtcatggcgg aagtcaaacg caaatggggc gaccgcttta ttgccgtcac
     1141 gcaaaaaaat accggtaaag gtggtgcgct aatgaatggc ctcaattacg ccacctgcga
     1201 tcaggttttt ttaagtgatg ccgacaccta tgttccgccc gatcaagacg gaatgggcta
     1261 tatgctggca gaaatagagc gcggtgctga tgccgtaggc ggcattccct ctactgcgtt
     1321 gaaaggcgcg ggtctgttac cgcacatccg cgcgaccgta aagttgccga tgattgttat
     1381 gaagcgcacg ctacagcagc tcctgggcgg cgcaccgttt attatcagcg gtgcctgcgg
     1441 gatgttccgt actgatgtat tgcgtaagtt cggtttctct gatcgtacta aagtcgaaga
     1501 ccttgatctc acctggacat tggtggcaaa cggctatcgt attcggcagg cgaatcgctg
     1561 catcgtatac ccacaggaat gcaacagccc gcgtgaggaa tggcgtcgct ggcggcgttg
     1621 gattgtgggc tacgcggtct gtatgcgcct gcataaaaga cttttattta gccgcttcgg
     1681 tatcttcagt atatttccta tgctgttggt tgtgctttat ggcgttggga tttatctcac
     1741 tacctggttt aatgaattca tcaccaccgg gccacatggt gtggtgttgg caatgttccc
     1801 gcttatctgg gtcggcgtag tttgtgttat tggtgcattt agcgcctggt ttcatcgttg
     1861 ctggttgttg gtgcctttag caccgctttc cgttgtgtat gtattattag cttatgccat
     1921 ctggattatt tatggactta ttgccttttt tactggacgc gaacctcagc gcgacaaacc
     1981 cacccgctat tccgcactgg tggaagcgtc aaccgcttat tcccaccctt ctgtcacagg
     2041 aactgaaaaa ctatctgaag cttaatgtta aagagaaaaa tattcagatt gcagaccagg
     2101 tgattattga tgaaagtgcc ggtgaggttg ttatcggcgc gaatacccgt atttgccacg
     2161 gtgccgttat tcagggtccg gtagtgattg gcgcaaactg cctgataggt aattatgcgt
     2221 ttattcgtcc tggcacaata atcagcaatg gcgtaaaaat tggttttgcc accgaaatta
     2281 aaaatgcggt tattgaagcg gaagcaacga ttggtccgca atgctttatt gccgactcgg
     2341 tagttgcaaa ccaggcatat ttgggcgcac aagtacgaac cagtaatcat cgtctggatg
     2401 aacagcccgt gtctgttcga actccagagg gaattatcgc taccggatgc gataaattag
     2461 gttgttatat cgggcagcgt tcacgccttg gcgtacaagt tattattttg cctgggcgaa
     2521 ttatttcccc gaacacgcaa cttggcccgc gcgtgattgt agaacgtaat ttacctaccg
     2581 gaacttattc actccgacaa gaacttatcc gtacaggaga ttaaaatgat aaaacggacg
     2641 ttattagcgg cggcaatttt tagcgcatta cccgtttatg ccgggttaac ttccattacc
     2701 gcgggctacg attttaccga ttattctggc gatcatggca accgtaattt agcgtatgct
     2761 gaactggtgg cgaaagttga aaacgcaacg ctgcttttta atctttccca ggggcgtcgt
     2821 gattatgaaa ctgaatattt caatgccact cgcggtcagg gtgcggtctg gtataaatgg
     2881 aataactggc tgacaacccg aacgggtatt gcctttgcgg ataatacgcc ggtctttgcc
     2941 cgccaggatt ttcgtcagga tattaacctg gccctgttgc caaaaacgct tttcacgacc
     3001 ggttatcgct acactaaata ttacgatgat gtcgaagtcg atgcctggca aggcggcata
     3061 tcactctata ctggcccggt gatctccagc taccgctata cccattatga ctccagcgat
     3121 gcaggtggta gttacagcaa tatgatttcc gtgcgtctga atgacccgcg cggcactggt
     3181 tatacgcaac tatggctaag ccgcggaaca ggcgcttaca cctatgactg gacgccagaa
     3241 acacgctacg gcagcatgaa gagcgtcagt ctgcaacgta ttcaaccgct gactgagcaa
     3301 cttaatctcg ggctgacggc aggtaaagtg tggtacgaca ccccaaccga tgattataac
     3361 ggtctgcaac ttgcagccca tctgacctgg aaattctaat tccttctgcc gcccgctatc
     3421 cggggcggcc ttccctgtcg attaggcccc tctttccttt ttgttatccg actacattca
     3481 ccggataaat tttattctcc agtgttatat actatagggg ggtatgtatt gacatatagc
     3541 atacccccct atagtatatt gcgtgcagat gatgaggtgc gaaatgccca gtactccgga
     3601 agagaagaaa aaggtcctta ctcgagttcg tcgtattcgg gggcagattg atgctctgga
     3661 acggtcgctg gagggtgatg ccgaatgccg tgccatactc caacagatcg ctgccgttcg
     3721 gggcgcggct aacgggctga tggcagaagt gcttgaaagc catatccggg aaacgtttga
     3781 ccgaaatgac tgctacagcc gcgaagtcag ccaatccgtt gacgacacta ttgaactggt
     3841 tcgtgcctat cttaaatagc tgaatctatt accatattga ggaagagcga gagatgaaat
     3901 cacgtgctgc cgttgcattt gctcccggta aaccgctgga aatcgttgaa attgacgttg
     3961 caccaccgaa aaaaggtgaa gtgctgatta aagtcaccca taccggcgtt tgccataccg
     4021 atgcatttac cctctccggc gatgacccgg aaggtgtatt cccggtagtt ctcggtcacg
     4081 aaggggccgg cgttgtggtt gaagtcggtg aaggcgtaac cagcgtcaaa cctggcgacc
     4141 atgtgatccc gctttacacc gcagagtgcg gcgagtgtga gttctgtcgt tccggcaaaa
     4201 ccaacctctg tgttgcggtt cgcgaaaccc agggtaaagg cctgatgcca gacggcacca
     4261 cccgtttttc ttacaacggg cagccgcttt atcactacat gggatgctct acattcagtg
     4321 aatacaccgt ggtcgcggaa gtgtctctgg ccaaaattaa tccagaagca aaccacgaac
     4381 acgtctgcct gctgggctgt ggcgtgacca ccggtattgg tgcggtacac aacacggcta
     4441 aagtccagcc aggtgattct gttgccgtat ttggtcttgg cgcgattggt ctggcagtgg
     4501 ttcagggcgc gcgtcaggcg aaagcgggac ggattatcgc tattgatacg aacccgaaga
     4561 aattcgatct ggcgcgtcgc ttcggtgcta ccgactgcat taacccgaat gactacgaca
     4621 aaccgattaa agatgtcctg ctggatatca acaaatgggg tatcgaccat acctttgaat
     4681 gcatcggtaa cgtcaacgtg atgcgtgcgg cgctggaaag tgcgcaccgc ggctggggtc
     4741 agtcggtgat catcggggta gctggttctg gtcaggaaat ctccacccgt ccattccagt
     4801 tggtcaccgg tcgcgtatgg aaaggttccg cgtttggcgg cgtgaaaggt cgttcccagt
     4861 taccgggtat ggttgaagat gcgatgaaag gcgatatcga tctggaaccg tttgtcacgc
     4921 ataccatgag cctggatgaa attaatgacg ccttcgacct gatgcatgaa ggcaaatcca
     4981 ttcgaaccgt aattcgttac tgatttcccg caggtttacc cgtccagttc agacggggtt
     5041 cttaatactc tccccgggca gccgcccggg ggattaacca tgagataatg actgatggaa
     5101 ctcattgaaa aacatgctag ctttggcggc tggcaaaatg tgtatcggca ttattcccca
     5161 tcactgaaat gtgaaatgaa tgtcggcgtc tatctcccac caaaagccgc gaatgaaaaa
     5221 ttaccggtgc tgtactggct ttcaggcctg acctgcaacg agcagaattt cattactaaa
     5281 tcggggatgc aacgttacgc ggcggagcac aacattattg ttgttgcgcc agacaccagt
     5341 ccgcgaggca gtcatgtcgc agatgccgac cgttacgatc tcgggcaagg tgccgggttt
     5401 tacctgaacg caacgcaagc gccgtggaat gaacattaca aaatgtatga ttatatccac
     5461 aacgagctgc cggatttagt gatgcaacat tttccggcaa cgaccagaaa gtctatctca
     5521 ggtcattcta tgggcgggct gggcgcgctg gtgctggcgt tacgtaaccc agatgaatat
     5581 gtcagcgtct cggcgttttc gcccattgtc tccccatcgc aagtaccgtg gggacagcaa
     5641 gcctttaccg catatcttgg tgaaaataaa gatgcctggt tggattacga tccggtgagt
     5701 cttatttcac aaggtcaacg cgttgcggaa ataatggttg atcaggggtt gagtgatgat
     5761 ttttacgcag aacagctgcg gactccaaat cttgaaaaga tctgccagga gatgaatatc
     5821 aaaacgttaa tccgttatca cgagggttat gatcacagct attattttgt ctccagtttt
     5881 attggcgagc atattgcgta ccacgccaat aaactgaata tgcgttgagc atccgcgcac
     5941 gcatgccgga tgcggcgtaa acgccttatc cggcctacat ttcacccgta agccgtaggc
     6001 ctgataagac gcgaaaagcg tcgcatcagg catctgcgca cggctgccgg atgcggcgtg
     6061 aacgccttat ccgtcctaca cttcgcccgt aaaccgtagg cctgataaga cgcgtaagcg
     6121 tcgcatcagg catcactgca cggcttatct atgtttacca catcaaatca tcaggcactt
     6181 tgaagtcggc atacggatcg tcttcatcct gctcttcggc gctgagcgcg ctgtgcaaca
     6241 caatactgct ggcatcacgc tgagcaattt tatcggcaac actagcggga ataatggcgt
     6301 attcaccttc gctattgtta tcgaccaaca agcgggcaat cgccagacga ccattaatca
     6361 actgcgcctg tgtgagctta tcgacaaaaa tcttcttaat cagattgccg tcagtgaagt
     6421 taaaaccaat atcgccattg gcaatggtga ttcggttcat ttcaatgagc tgcttcacct
     6481 gggctttata ttctttcgcc aacgccgctt gtttttgctg ttcgctaagc tgtttatcac
     6541 gctcaagctg tgccttttta ttttcttcta ccgccgcccg agcttcacgc gcctgaacgc
     6601 gcgatttttt cgccgttctc tccaccttcg ctgctttttt gctggtgact aatcctgctt
     6661 tgagcaactg ctcttgtaag gtaagttttg ccatctttgt ttccaaaccc gctgaataat
     6721 ttttagcgag tatacctgta aacgccgtag gtgtattggg ggcaagtccc tttcgcccct
     6781 gcttaataag gcgcgtcagg catcggcaca cggctgcatt cttgatctcc gactcatcaa
     6841 aataaacact gccaggccag caacaagaat acccggtgca gaagcggcca ttacgccgac
     6901 cgtgccagtg cctaatgcca gcattttccc ggccagtaac ggaccgctca tagcccccag
     6961 acgccctacc gccacggctg ttcccacacc tgttgcgcgg atctgcgaac tgtaaaacaa
     7021 cggtgccagg gcatacaaaa cgctttgccc acctgtcgca aacaaccccg cgacgaatcc
     7081 cgccagcaac ataccgttaa atgacgacac cgttcccagc gccagcagcg aagctaacat
     7141 gccgctataa atcagtagcg acatggttac tggacgcagc ttatccatca atgcgcccaa
     7201 cattaacgtc ccgcttgccg cacccatttg cagtgcaaac atcacccctg ccgcctgcga
     7261 tggctggaat ccttgctcca ccaaaagcag cggtagccag ttgatcaaca tgtagaccac
     7321 cagcagagtg aagaaataac acaaccacag cagcagcgtc gcggttgccg tttctggcgc
     7381 aaataacgca cgcagtggag gcgcggcctg tttttcgcca gcgaacaccg ccgactccgg
     7441 cagccagcgc attaatagcg gcaccagaat caacggcacc acaccaccta cccaaaacac
     7501 cgtttgccat gctaagtttg cccccgcgaa acccagtgtc gccgccagcg ccgcgccaat
     7561 gggaacaccg caatacatca ggctcactgc cgtcccacga aaacgtggac ccgcggcttc
     7621 agacgtcagg gcgatcagat tcggcaacgc cgcccccagc ccgacaccgg tcatcagtcg
     7681 cgcaaagacc agtgagggga aatcccaggc aatcgccgtt gccagtgaga acaaaccaaa
     7741 cagcgcaact gagccaatca aaatgcgctt gcgaccataa cggtccgcca gcattccgcc
     7801 aaccaacgcg ccgggtagca aaccgagtat tccggcgctg aatatccagc ccatttgcat
     7861 tttatcgagt gcgaaagcct gggcgatgcc acccgccgca atgccagccg cctgaagatc
     7921 cagtccttcc atcagagcga ccaaaaaaca aagcccgatg gtcagcatca ggcgggttga
     7981 agatgatgaa ggggtacgag tcgacataag atgaacctcg ttgcgagtgc aggtgaatcg
     8041 cggctgatac attgtcagcc gcgtaatcag aaccatttaa tgactgccta cgggcgaaat
     8101 ataggccgga taaggcgttt acgccgcatc cggcagtcgt gctgggatgg gcatacaaaa
     8161 tgcacgttct cggcagtcat cattatttgt tgttgcgcag atccagcgcc acgtcgacga
     8221 tcatatcctc ctggccgcca accatccggc gtttgcccag ctcaacgaga atatccaccg
     8281 cgcttaagcc ataacgcgcc gccgccgttt cacagtgacg caggaagctc gagtaaacac
     8341 cagcgtatcc cagcgccagc gtttcgcgat cgactcgtac cggtcgatcc tgcaacggac
     8401 gcaccaggtc gtcagcggca tccattaacg catagagatc ggtcccatgc tgccaaccca
     8461 gtttatccgc ggcggcaata aaaacttcca gcggcgcgtt acctgcgccc gcgcccattc
     8521 ccgcgaggct ggcgtcgatt cggtcgcagc cctcttccac cgccgcgata gagttcgcca
     8581 cgccaagact caggttatgg tgagcgtgca tgccggtttg cgtttctggt ttcagcactg
     8641 ctttcagggc gcggaaacgg tcacggatat cgctcatgtt catcgcaccg ccagaatcca
     8701 ccacataaat acaggtcgca ccgtagcctt ccatcagctt tgcctgcttt gcgagattct
     8761 ccggcgtggt catatggctc atcatcagaa aaccaacggt gtccattccg agctcgcggg
     8821 catactgaat atgctgggcg gaaacatcag cttcggtgca gtgcgttgcc acacgaacca
     8881 cccgcgcgcc agcctgccag gcatttttca gatcgtggat agtgccgatt cctggcagca
     8941 acaacgtcgc gattttggcg tgcttcacca catccgccac tgcttcaatc cattcaaggt
     9001 cgctatgtgc gccgaaacca tagttaaagc tggacccctg caaaccgtcg ccatgggcca
     9061 cttcaatcga atccacgcgg gcatcgtcca gtgctttggc aatctggcga acgttttcca
     9121 gcgaatactg atgacgaatg gcgtgcatac cgtcacgcaa cgtgacgtcc gagatataaa
     9181 gttttttacc gttcatgccg cttctcctgc cttgcgcgcc agtgactggg ccattttttc
     9241 cgctgtcgcc agcgcactgg aagtcataat gtcgaggttg cctgcatagg caggcagata
     9301 atgcgctgcg ccttcgactt ccagccagac cgccgttttc agtccggaga actgccccac
     9361 gcccggcaaa ttaaccggtt tatcctgcgg gataacttca aactgcacgc gctgtttcag
     9421 gcgataaccc ggtacgtaag cctgcaccgc ctcagccatt tcattgattg aggcttcgat
     9481 atcatcttgt gaagcttcgt cgctcaatac atacaccgtg tcacgcatca tcaacggtgg
     9541 ctctgctggg ttaagcacaa taatcgcctt cccttttgcc gcgccaccca ccacttcaat
     9601 ggcgcgcgaa gtggtttccg taaactcatc gatattggca cgcgtgccag gtccggcaga
     9661 tttactggca atagaagcga taatttcggc gtaatgaaca cgcgccacgc gtgaaactgc
     9721 cgccaccatt ggaatggtgg cctggccgcc gcaggtgacc atgttgacgt tcagttgagc
     9781 gacgttctcc tcgaggttaa cgaccggcac gcagtagggg ccgatggcag caggcgtcag
     9841 gtcaattaag cgaatatccg gtttcgcttc gcgcaaagcg gcatcgtttt tcacatgcgc
     9901 acccgcgctg gtcgcatcaa atacaatgtc gatatcagca aattcaggca tgttcatcag
     9961 cccgatcacc ccttcatggg tggtggcgac gcccatacgt ctggcacgcg ccagaccgtc
    10021 ggactgagga tcaatgccga ccatcaccgc catctccaga tgctggccgt gacgcaaaat
    10081 tttaatcatc agatcggtgc caatgttgcc tgaaccgata atggcgactt tacgcttact
    10141 catgacagat ttccttttgg ggctgcgctt gaaaatgtcg cagcaactga acctatgcct
    10201 tcaatatggg cttcaaaacg atcgcccgca ttcaccgcca ccatcggacc taatgccccg
    10261 gtaagaatga tatctccggc gcgcagcggt tcacccaggc tggccatttt gcgtgccagc
    10321 cagacggcgg cattaagcgg atgtcccagg cattcgctgc cgcgcccgct ggaaacctct
    10381 tcgttattac gcgtcatctt catggcgcag tttttcaggt ctaaccccgc cggacgttgc
    10441 gccggaccgc cgatgacata caccccgcag gaagcgttat ctgccacggt atcgacaaac
    10501 tgaatcgacc agtcgcgaat gcggctcccc accacttcca gcgccggaag tacccattca
    10561 atggcgttat acaattcgtc gaaggtgata tcggttgcgg gtagatcgcg gttcaacacc
    10621 agtgcaatct ccgcttcaat gcggggttgc agaacacggg aaaaaggaat ggtttcgtta
    10681 tcgccataac acatgtcggc aaataatgtc ccgaaatccg gttgatcaac gcccagttgt
    10741 tgctgcactt tcggatgcgt cagccccact ttgcgcccca ccactcgccg tccctgagcg
    10801 acgtcgtgtt gcacattgat gtgctgaatg gcgtaggccg cttcagcgtt atcgatacca
    10861 atcagatcgc gcagcggcgc aatcgcttcg ccctgctctg cggcgcgacg taaatccgcc
    10921 gccagttgct caagagtatg cttcgtcatg accgttcctt aagggcgtgc gaggaaattc
    10981 agcaccagtt gattgaaagc gtcggcatgt tcccactgcg cccagtgacc acagtcgcgg
    11041 aagatatgca gttcagaacc ggcaatgccg gacagcagac gcagacccgc atccatcggc
    11101 acaaagcggt cgttgcgccc ccagacaatc agggtttgcg ctttgatttc cgccagacgt
    11161 gggccaaaat ccgggaactg tttcggattg gcttccaggc tcttaacgaa gttttccagg
    11221 tgatcgcggc gcgacagcat attattcagg cgcgcttcaa acagggcgtc ggtcaaatcg
    11281 ctggtatcaa aaacgaagat atccatcatc agcttcaggt tttcgatagt cggctgacga
    11341 taaagctgat tcagtcgctt aataccttcg gttggcatcg gcgtaaacaa actcatgccg
    11401 cccgtaccgc cgcccatcag caccagtttg ccgacgcgct ccggccagtt aagggtgaac
    11461 gccacagaac tgtggccgcc catcgagttg cccagcaggt ggattttggc gatatccagt
    11521 tgatccacca cgcttttcag gattcgcgca ttaagatccg atcgtgaacc actattaacg
    11581 atcgaatcgc tcttacccca acccggacaa tccagcagga tcacccgata gcccgccttt
    11641 accagcggat cgatattgcg gctgaagttc gcccagccag tagcacccgg gccggaacca
    11701 tgcagcagga caacagtttc gtcgccttgt ccgcagtcat taaaatggat gcgcagcgtt
    11761 ttacccgctt cttctacatt cagaaaacgg ctggtggcgg cttcggtttg tggctgataa
    11821 ctcatcatct tctcctgcat attcagttct ctgttctggc gcttaacgag ccaaatccgg
    11881 caatccactc cgggattggg cggtaataac gcccttcgct acgccagttg ccaaacgcag
    11941 aaatagcggc aaaagcggcg acccaggttt tgatttcatg tgtcgacttt ccggcaatgg
    12001 cggaaagctc ttcgttactg acggcatcca gttcctgtat gcgtccctgc tccagcaaag
    12061 tcatgaactg gttatcccaa atcgggttga gcggatgcag cgttctctga tcctcaacaa
    12121 acttctcagc ggcgctaatc acccgctgct gacgcaattc gcgctcactg gcgggtaaat
    12181 ctttcccgct ccccaacaga cggtcgcgca tatgggcatc ggctttcgcc agttctggta
    12241 ccggtggctg atgggaaagc ccaccggacc caaggaacag cacgcgttta ttgagagtgc
    12301 tggtgaaacg tccaatggct tcccccaaca tgcgggtacg ctggaaaccg ggcaacggcg
    12361 tggcgacacc gttgatgaac acaggcagaa ccggcacctt atccagccca ccgagcagga
    12421 actccagcgg ctgggcgaac ccgtggtcca cctgcataca gtaagaaacg gcaagatcga
    12481 ttccgctctt catgacggca tgcgcacagg cctccgccag ctcaacaggc acgggcagct
    12541 cacctgccgc actgccgaaa tcaccaattg ccgtcgctcc aacgcctaaa cagaacggtg
    12601 gcatcacgtc atagaaaaag ccgttgtagt gatctggcgc aaacagcacc accagttcag
    12661 gggagaaagc cgcaatacgc tcgcgggcgc tggcaatcac gccattgacc tcatcgagta
    12721 cctcttgcgc cgggtcgaca tatcccacca gcggcgagtg ggaaagacag tgaagataag
    12781 cgtgcatatc aggctacctt ttcgacagaa acgtcggcat cagggcgggt cagcgtcatt
    12841 accgacgcca gtttattcag cgtattaccc agagtttgcg gaatggcggt ggcggcaaca
    12901 aagcgatccg ggcgcatcac caccagcgaa gcattgtgtt gcgcgaacca gctacgcagg
    12961 cgaccttgcg tatcgcccac gcgtagtacg ccgtcgtggt tatcctgtgc ggtatgaatt
    13021 tgcacttccg gcaccacctg aatgaagcgt gtgcccaacg cgcgccactg ctggatttgc
    13081 tcgtcgctca tcccccacag tggattgcat ccccagccaa ttaccgcgaa gttcgcgccg
    13141 atcgcgttat cgagcagcgt cacgtcgccg ttttccagcg tgactttcgg ctgaataaac
    13201 atcttgccga ccggagagtg cttcgcttcg ccctcacgca ccagcgcacc gccgtaatat
    13261 tgcggcatcg gcttgaagcg catttcgagg aagtagcgtt ttactggcgg cagataattc
    13321 aacagccagg aaacgccgtc acgtaacgta ccctgccagc gtttcggcgg agccagcacg
    13381 ttgcccgccg tcacggacag gtcaatcatc gctttggcgt gatcgcgacg ttcttgttga
    13441 taggtatcga gcagcgcatc gcgggctttc cactggataa ccaacgccag tttccatgcg
    13501 aggttaaagg cgtcgcgcat gccactgtta tagccctgcc cctgccatac cggcatgatg
    13561 tgcgcggcat cgcccgccag cagtacgcga tcaatacgaa aacgttgcgc cagtcgcgcg
    13621 ttgtgggtgt agacacgctg gcgaatcaat tcaacattgt ccggattagg cagcactttg
    13681 cttaacagct tgcgcatatt ttgcggctca cgcagctgtt cttcggtttc tcccggcatc
    13741 accataaatt caaagcgacg taccgcatga ggcagcgcgg cagaaacata cgggcgcacc
    13801 ggatcacaac acaaatagat atgcggcgta cttaacggat cgttggcgat atctaccaca
    13861 atccactgat ttggcgcagt tttaccttca aacggcacat tcagggtgcg acggacaaaa
    13921 cttgccccac catcacaggc caccagccat tgggctttga ccgtttcccg ctgcccttct
    13981 gccgttttca ggtgcaaggt cacttcgtca tcttgctggc tgaaggcctc cagctcgcgg
    14041 gaaaacaggc agcgcacatt cggaaaacgc gacaatcctt ccagcatcac cgcatcgacc
    14101 tgaggctgat taaaggcgtt acggcgcggc cagccaaatt catcggtcat tggctgaata
    14161 tcagcaaaac agcgaccttt tggggtgaga aaacgcatcg cgtgccacgg cgtagtgtgc
    14221 ggcagaacat tatcgaccag gccgaccgac tgcatggtgc gcagcgcctc gtcatcaata
    14281 ccaatcgcac gcgggtagtc gatcaactta tcgagtttct ccaccaccag cacgtcaatg
    14341 cccatctggc cgagatagtt cgccatcatc agcccgaccg gaccggcacc agcgatcgcc
    14401 acctgaacgc tatggttaac agcaggctgg atgtcaggat gttgtattgc catttcagta
    14461 cctcacgact cggacaaaat gccgttgcgc gcacagtacg gcgcaactta ttttgttaaa
    14521 aacatgtaaa tgatttttta ttgtgcgctc agtataggaa gggtgttttc ggctacaatc
    14581 aaaacatgcc cgaatgtgca ccaagtgcac aacgttgttt taactataga aatgtcaatt
    14641 aatatgcaga acaatgagca gacggcatac aaaaccgtgc gcggcttaac ccgcggtcta
    14701 atgttattaa atatgttaaa taaacttgat ggcggtgcca gcgtcgggct gctggcggaa
    14761 ctcagcggcc tgcatcgcac cactgtgcgg cgactactgg agacgctgca tgaagaggga
    14821 tatgtccggc gtagtccctc cgatgatagt ttccgtttga ccatcaaagt gcggcaatta
    14881 agcgaaggat ttcgtgacga acagtggatt tctgcactgg cggccccact gctgggcgat
    14941 ctgttgcgcg aagtggtatg gccgacagat gtgtccaccc tggatgttga tgcaatggtg
    15001 gtacgcgaaa ctacccatcg tttcagccgc ttatcttttc accgggcaat ggtcgggcga
    15061 cggttaccgc ttctgaaaac cgcctcgggc ctgacctggc tggccttttg cccggaacaa
    15121 gaacgcaagg aattaatcga gatgttagcc tcccgccccg gtgatgacta tcaactggca
    15181 cgggaaccgt taaagctgga agccattctg gcgcgcgcgc gcaaagaggg gtacggacag
    15241 aactaccgcg gctgggatca agaagaaaag atcgcctcta ttgccgtacc gctgcgcagt
    15301 gaacaacggg tgattggctg tctgaatctg gtgtatatgg cgagcgcaat gaccattgaa
    15361 caggcagcgg aaaagcatct tccggcgcta caacgggtgg caaaacagat cgaagaaggg
    15421 gttgaatcgc aggctattct ggtggccgga cggcgaagcg gcgtgcattt acgttgacac
    15481 catcgaatgg cgcaaaacct ttcgtggtat ggcatgatag cgcccggaag agagtcaatt
    15541 cagggtggtg aatgtgaaac cagtaacgct atacgatgtc gcagagtatg ccggtgtctc
    15601 ttatcagacc gtttcccgcg tggtgaacca ggcctgccac gtttctgcga aaacgcggga
    15661 aaaagtggaa gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg cacaacaact
    15721 ggcgggcaag cagtcgttgc tgattggcgt tgccacctcc agtctggccc tgcacgcgcc
    15781 gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt
    15841 gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc
    15901 gcaacgcgtc agtgggctga tcattaacta tccgctggat gaccagaatg ccattgctgt
    15961 ggaagctgcc tgcgctaatg ttccggcgtt atttcttgat gtctctgacc agacacccat
    16021 caacagtatt attttctccc atgaagacgg tacgcgactg ggcgtggagc atctggtcgc
    16081 attgggtcac cagcaaatcg ctctgttagc gggcccatta agttctgtct cggcacgtct
    16141 gcgcctggcg ggctggcata aatatctcac tcgcaatcaa attcagccga tagcggaacg
    16201 ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg
    16261 catcgttccc actgcgatgc tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc
    16321 cattaccgag tccgggctgc gcgttggtgc ggatatctcg gtagtgggat acgacgatac
    16381 cgaagacagc tcgtgttata tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct
    16441 ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagggcaa
    16501 tcagctgttg cccgtctcac tggtggaaag aaaaaccacc cttccgccca atacgcaaac
    16561 cgcttctccc caggcgttgg cagattcttt aatgcagctg gcgcgacaaa tttcccgact
    16621 cgaaagcggg cagtgagcgc aacgcaatta atgtgagtca gctcactcat taggcacccc
    16681 aggctttaca cattatgcgt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
    16741 ttcacgcagg atacagctat gactatgatt acggattcac tggccgtcgt attacaacgt
    16801 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcggcaca tccccctttc
    16861 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
    16921 ctgaatggtg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg
    16981 ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac
    17041 ggttacgatg cgcccatcta caccaacgtg acctatccca ttacggtcaa tccgccgttt
    17101 gttcccgcgg agaatccgac gggttgttac tcgctcacat ttaatattga tgaatgctgg
    17161 ctacaggaag gccagacgcg aattattttt gatggtgtta actcggcgtt tcatctgtgg
    17221 tgcaacgggc gctgggtcgg ttacggccag gacagccgtt tgccgtctga atttgacctg
    17281 agcgcatttt tacgcgccgg agaaaaccgc ctctcggtga tggtgttgcg ctggagtgac
    17341 ggcagttatc tggaagatca ggatatgtgg cggatgagcg gcattttccg tgacgtctcg
    17401 ttgctgcaca aaccgaccac acaaatcagc gatttccatg ttgccactcg ctttaatgat
    17461 gatttcagcc gcgcggtact ggaggcagaa gttcagatgt gcggcgaact gcgcgatgag
    17521 ctgcgggtga cggtttcttt gtggcagggt gaaacgcagg tcgccagcgg caccacgcct
    17581 ttcggcggtg aaattatcga tgagcgtggt ggttatgccg atcgcgtcac gctacgtctg
    17641 aacgtcgaaa acccggcgct gtggagcgcc gaaatcccga atctctatcg tgcggtggtt
    17701 gaactgcaca ctgccgacgg cacgttgatt gaagcagaag cctgcgatgt cggtttccgc
    17761 gaagtgcgga ttgaaaatgg tctgctgctg ctgaacggca agccggtgct gattcgcggc
    17821 gttaaccgtc acgagcatca tcctctgcat ggtcaggtca tggatgagca gacgatggtg
    17881 caggatatcc tgctgatgaa gcagaacaac tttaacgccg tgcgctgttc gcattatccg
    17941 aatcatccgc tgtggtacac gttgtgcgac cgctacggcc tgtatgtggt ggatgaagcc
    18001 aatattgaaa cccacggcat ggtgccaatg aatcgtctga ccgatgatcc gcgctggcta
    18061 cccgcgatga gcgaacgcgt aacgcgaatg gtgcagcgcg atcgtaatca cccgagtgtg
    18121 atcatctggt cgctggggaa tgagtcaggc cacggcgcta atcacgacgc gctgtatcgc
    18181 tggatcaaat ctgtcgatcc ttcccgcccg gtgcagtatg aaggcggcgg agccgacacc
    18241 acggccaccg atattatttg cccgatgtac gcgcgcgtgg atgaagacca gcccttcccg
    18301 gctgtgccga aatggtccgt caaaaaatgg ctttcgctgc ctggagaact gcgcccgctg
    18361 atcctttgcg aatatgccca cgcaatgggt aacagtcttg gcggcttcgc taaatactgg
    18421 caggcatttc gtcagtaccc ccgtttacag ggcggcttcg tctgggactg ggtggatcag
    18481 tcgctgatta aatatgatga aaacggcaac ccgtggtcgg cttacggcgg tgattttggc
    18541 gatacgccga acgatcgcca gttctgcatg aacggtctgg tctttgccga ccgcacgccg
    18601 catccggcgc tgacggaagc aaaacaccag cagcagtttt tccagttccg tttatccggg
    18661 cgaaccatcg aagtgaccag cgaatacctg ttccgtcata gcgataacga gctcctgcac
    18721 tggacggtgg cactggatgg caagccgctg gcaagcggtg aagtgcctct ggatgtcgct
    18781 ccacaaggta aacaggtaat tgaactgcct gtactaccgc agccggaaag caccgggcaa
    18841 ctctggctaa cggtacacgt agtgcaaccg aacgcgaccg catggtcaga agccggacac
    18901 atcagcgcct ggcatcagtg gcgtctggcg gaaaacctca gcgtgacact ccccgccgcg
    18961 tcccactcca tcccgcatct gaccaccagc gaaacggatt tttgcatcga gctgggtaat
    19021 aaacgttggc aatttaaccg ccagtcaggc tttctttcac agatgtggat tggcgatgaa
    19081 aaacaactgc tgacgccgct gcgcgatcag ttcacccgcg caccgctgga taacgacatt
    19141 ggtgtaagtg aagcgacccg cattgaccct aacgcctggg tcgaacgctg gaaggcggcg
    19201 ggccattacc tggccgaagt ggcgttgttg cagtgcacgg cagatatact tgccgacgcg
    19261 gtgctgatta cgaccgctca cgcgtggcag catcagggaa aaaccttatt tattagccgg
    19321 aaaacctacc gtatcgatgg acacggtgag atggtcgtca gtgtggatgt tgaagtagcg
    19381 agcgatacac cgcatcctgc acggattggc ctgacctgcc agctggcgca ggtcgcagag
    19441 cgggtaaact ggctcggatt agggccgcaa gaaaactatc ccgaccgcct tactgcggcc
    19501 tgttttgacc tctgggatct gccattgtca gacatgtata ccccgtacgt cttcccgagc
    19561 gaaaacggta tgcgctgcgg gacgcgcgaa ttgaattatg gcccacacca gtggcgcggc
    19621 gacttccagt tcaatatcag tcgctacagc caacaacaac tgatggaaac cagccatcgc
    19681 catctgctgc acgcggaaga aggcacatgg ctgaatatcg acggtttcca tatggggatt
    19741 ggtggcgacg actcctggag cccgtcagtg tcggcggaat tccagctgag cgccggtcgc
    19801 taccattacc agttggtctg gtgtcaaaaa taataataac cgggcaggcc atgtctgccc
    19861 gtatttcgcg taaggaaatc cattatgtac tatttaaaaa acacaaactt ttggatgttc
    19921 ggtttattct ttttctttta cttttttatc atgggagcct acttcccgtt tttcccgatt
    19981 tggctacatg acatcaacca tatcagcaaa agtgatacgg gtattatttt tgctgctatt
    20041 tctctgttct cgctattatt ccaaccgctg tttggtctgc tttctgacaa actcgggctg
    20101 cgcaaatacc tgctgtggat tattaccggc atgttagtga tgtttgcgcc gttctttatt
    20161 tttatcttcg ggccactgtt acaatacaac attttagtag gatcgattgt tggtggtatt
    20221 tatcttggct tttgttttaa cgccggtgcg ccagcagtag aggcatttat cgagaaagtc
    20281 agccgtcgca gtaatttcga atttggtcgc gcgcggatgt ttggctgtgt tggctgggcg
    20341 ctgtgtgcct cgattgtcgg catcatgttc accatcaata atcagtttgt tttctggctg
    20401 ggttctggct gtgcactcat cctcgccgtt ttgctctttt tcgccaaaac ggatgcaccc
    20461 tcttccgcca cggttgccaa tgcggtaggt gccaaccatt cggcatttag ccttaagctg
    20521 gcgctggaac tgttcagaca gccaaaacta tggtttttgt cactgtatgt tattggcgtt
    20581 tcctgcacct acgatgtttt tgaccaacag tttgctaatt tctttacttc gttctttgcc
    20641 accggtgaac agggtacgcg ggtatttggc tacgtaacga caatgggcga attacttaac
    20701 gcctcgatta tgttctttgc gccactgatc attaatcgca tcggtgggaa aaacgccctg
    20761 ctgctggctg gcactattat gtctgtacgt attattggct catcgttcgc cacctcagcg
    20821 ctggaagtgg ttattctgaa aacgctgcat atgtttgaag taccgttcct gctggtgggc
    20881 tgctttaaat atattaccag ccagtttgaa gtgcgttttt cagcgacgat ttatctggtc
    20941 tgtttctgct tctttaagca actggcgatg atttttatgt ctgtactggc gggcaatatg
    21001 tatgaaagca tcggtttcca gggcgcttat ctggtgctgg gtctggtggc gttgggcttc
    21061 accttaattt ccgtgttcac gcttagcggc cccggtccgc tttctctact gcgtcgtcag
    21121 gtgaatgaag tcgcttaagc aatcaatgtc ggatgcggcg cgagcgcctt atccgaccaa
    21181 catatcagga cggaatgatc gcaatgaaca tgccaatgac cgaaagaata aaagcaggca
    21241 agctatttac cgatatgtgc gaaggcttac cggaaaaaag acttcgtggg aaaacgttaa
    21301 tgtatgagtt taatcactcg catccatcag aagttgaaaa aagagaaagc ctgattaaag
    21361 aaatgtttgc ctcggtaggg gaaaacgcct gggtagaacc ccccgtctat ttctcttacg
    21421 gttccaacat ccatataggc cgcaattttt atgcaaattt caatttaacc attgtagacg
    21481 actacacggt aacgatcggt gataacgtat tgattgcacc caatgttact ctttccgtta
    21541 cgggacaccc tgtacaccat gaattgagaa aaaacggtga gatgtactct tttccgataa
    21601 cgattggcaa taacgtctgg atcggaagtc atgtggtcat taatccaggc gtcaccatcg
    21661 gggataattc tgttattggc gcgggtagtg tcgtcataaa agacattcca ccaaacgtcg
    21721 tggctgctgg cgttccttgc cgggttattc gcgaaataaa cgaccgggat aagcactatt
    21781 attacaaaga ttataaagtt gaatcgtcag tttaaattat aaaaattgcc tgatgcgctc
    21841 cgcttatcag gcctacaagt tcagtgatct acgtaggccg catccaacgt aaacaaagcg
    21901 caggaacaag cgtcgcatca tgcctctttg acccacagct gcggaaaacg tgctggtgca
    21961 aaacgcaggg ttatgatcat caacccaacg acgcacagcg catgaaacgc ccagtccatc
    22021 aagtaattgc cactgatact acgcagcaca ccagaaaacc acggggcaag cccggcgatg
    22081 ataaaaccga tcccctgcat aaacgccacc agtttgccag caatagcggg ttgcgcagag
    22141 tgatcgagtg ccagcagcaa acagagcgga aatgcgccgc ccagacctaa accacacacc
    22201 atcgcccaga agaccggcaa ttgcagcggc agccagataa agccgcagaa acccaccagt
    22261 tgtaacgcca gcgccagcat caaaagtttg cgccgatcct gatggcgagc catagcaggc
    22321 atcagcaatg cccccgcagc ttgcccaagc gtcatcaacg ccagcaagga accgctgtac
    22381 tgcgcgctgg caccaatctc aatatagaaa gcgggtaacc aggcaatcag gctggcgtaa
    22441 ccaccgttaa tcagaccgaa gtaaacaccc agcgtccacg cgcggggagt aaataccacg
    22501 cgaaccggag tggttgttgt cttgtgagaa gaggcgacct cgcgggcgtt ttgccaccac
    22561 caggcaaaga gcgcaacaac ggcaggcagc gcccaccagg cgagtgtttg ataccaggtt
    22621 tcgctatgtt gaactaacca gggcgtaatg gcggcaccaa gtccaccgcc gcccattaga
    22681 gccgcggacc acagccccat caccagtggc gtacggtgct gaaaccgccg cttaattacc
    22741 gaaggcatca ctgcctgaat gatgccgatc cccactccac caagcagtgc gctgctaagc
    22801 agcagcgcac tttgggggta aagctcacgc atcaatgcac cgacggcaat cagtaacaaa
    22861 ctgatggcga cgctgcgacg ttcgctgaca tgctgatgaa gccagcttcc ggccagcgcc
    22921 agcccgccca tggtaaccac cggcagagcg gtcaacaggg cagccacgct aaagctcatt
    22981 ccgcttgcct ggcgcaattg cggtagcagt ggcccgacgg aggtgagcag tggtcgcata
    23041 ttaagaccaa tcagcaccag aaccagcagc atcgtgaacg tcttttgatg gttacgcatg
    23101 gctgtcagaa cggtttggtc ggcagatatt taccatctaa ggtgatgacc gcacgttcgc
    23161 caccttccgg gtccgccact ttcttaacgt cgagtttgaa gttaatcgcg ctgataatgc
    23221 catcgccaaa cttctcatga accaacgctt tcagggttgt gccgtacacc tgcaacattt
    23281 catagaaacg atacatcgtt gggtcggttg gaatacggtc atcaatgcag ccacgcactg
    23341 ggatcatctg taacagtaga atggcgtctt cgtcgagatc cagcttcgcc ccgacctggc
    23401 gggcggcgtc ggcaggaagc gcctgctgac ccagcaaagc cgcggtcaca aaggcttctg
    23461 ccagcccggt gccgtcggca atctcggcaa atgagagatc ttttttagct ttgctgagca
    23521 aaatggcatc ggcaagatca agacgaatat tgcggttaat ttgtgactga atcatggtgg
    23581 aactcctgat ggtttaaaaa taaggttacg ctgcggtcgg ttggcgtagc ggtatggcac
    23641 aaacgcgagg attagcggcc agtggcacaa actggcgggt tgcgccgtca aaagctgcga
    23701 tgctgccgct ttcaatgtcg tagacccagc cgtgcagggc gatccgcccc tcttcgagcg
    23761 ccaggcgcac cgatggatga gtttgcaaat tagccaactg agcaatgacg ttttcacgta
    23821 ccatcgccgc agcttttgac ggtaaatcgg aatgcgggcg cgcctcatta acgacgcggg
    23881 ctgaatcggc ataacgcagc cagtgggaga cggcaggcat atggtccatg cactgacagc
    23941 tggcaatgga ggtcatcgcg ccacagttgg aatgaccgca aatcacaatg tcagataccc
    24001 gaagcgcagc gacggcatac tccaccgaag cagaaacgcc accgggttcc ggcccgtagg
    24061 aagggacgat attgcccgcg ttgcgaataa cgaacagatc gccaggctca cgttgcgtca
    24121 ccagctcagg gaccagacgg ctgtcggagc aggagataaa aagtgtgcgc gggctttgct
    24181 gtgtcgccaa ctgtttaaat aaggcttccc gcttcggaaa tgcctcgcgc tggaatttaa
    24241 ggaatccatc aataatctct ttcaccgtta acctctgtct gtctctggaa tgagaggcag
    24301 actactcgcc gattgtcata aggtaaaagt ctcatttatg atgagtccta ttggatttac
    24361 ttataggttg cgaatgctct ctcgacatat caattatttt cttgccgtgg ctgaacatgg
    24421 cagcttcacc cgtgccgcca gtgcgttgca cgtctcccag cctgcgcttt cccagcagat
    24481 tcgccagtta gaggagagtt taggcgtgcc gctgtttgac cgtagcgggc gaacgattcg
    24541 tctcactgat gcaggagaag tctggcgaca gtacgccagc cgggcgttac aggaactggg
    24601 ggcgggtaaa cgggcgattc atgatgttgc cgatctgacg cgaggatcgc tgcgtatcgc
    24661 cgtcaccccc acctttacga gctactttat cggcccctta atggcggatt tctatgcgcg
    24721 ctatcccggc atcacgctcc agctacagga aatgtcgcag gagaaaatcg aagatatgct
    24781 ttgccgcgac gagttggacg ttgggattgc cttcgcgcct gtgcattcgc cggagctgga
    24841 ggcaattcct ttactgacag aaagtttagc gttagtcgtg gcgcaacatc atccgctggc
    24901 cgtccatgaa caggtggcgt tgagtcgctt gcatgatgaa aaactggtcc tgctcagcgc
    24961 ggaatttgcc accagagagc aaattgacca ctactgcgag aaagcggggc tgcatccaca
    25021 ggtggtcatt gaggcgaact caattagcgc ggttctggag ctgattcgcc gcacttccct
    25081 ttccacattg ttaccagcag cgattgccac acaacatgac gggcttaaag ctatttctct
    25141 tgccccgcca ctactggaga gaacggcggt tttgttgcgg cggaaaaata gctggcagac
    25201 tgccgccgcg aaggcatttt tgcaaatggc gttggataaa tgcgcggttg ttggcggaaa
    25261 tgaatcgcgg taaccactga cacaattgcc tgatgcgcta cgcttatcag gcctacgagt
    25321 tcagtgctct acgtaggccg gataaggcgt tcacgccgca tccggcataa actacgctcg
    25381 ctttgtcaac acacagccca gccgatcacc gtttgtaatc gatggcttct ggctgctcca
    25441 gatatacggt ggtttgtgcc ggttgtgtgc tggcaatcac cttgccgcca cgtaccgaat
    25501 aacgtaccgg aacctgacgg cgcagcgcat caaatccatt ttcagccggc aggataatca
    25561 ggttggcgct gtttccggcg gcaatgccgt aatcctgcaa attcaacgtt ctggcactgt
    25621 ggtgggtgat taaattcagg ccatcgttaa tctgtccgta gcccatcagc tggcaaacat
    25681 gcagccccat atgcagcact tgcagcatat tcgccgttcc cagcggatac cacggatcga
    25741 agacatcatc gtgaccaaag cagacgttaa tgccggactc cagcatctct ttaacgcgcg
    25801 tgatgccgcg acgttttgga tacgtatcga atcgtccttg cagatgaata ttgaccagcg
    25861 ggttggcgac aaagttaata ccggacattt tcagcaagcg gaacaggcgt gaggtatacg
    25921 ccccgttata ggagtgcatt gccgtggtgt gactggcggt gactcgcgcg cccatgcctt
    25981 cacggtgcgc cagggcagca acggtttcga caaagcgcga ctgctcgtca tcgatctcat
    26041 cacagtgaac gtcgatgaga cggtcgtatt tttgcgccag ggcgaaggtt ttatgcagcg
    26101 actccacgcc gtattcacgg gtaaattcaa aatgcggaat cgcccccact acatctgccc
    26161 ctaagcgtaa cgcctcttcc agcaacgctt caccgttggg atacgacaaa atcccttcct
    26221 gagggaaggc gacgatttgc agatcaatcc acggcgcgac ttcctgcttc acttccagca
    26281 ttgctttcag cgcagttagc gttgcatccg aaacatcgac atgggtacgc acatgctgaa
    26341 tgccgttggc aatctgccat ttcagcgttt gccatgcgcg ttgtttcaca tcgtcatggg
    26401 ttaataacgc tttgcgctcg gcccagcgtt caatgccttc aaacagcgtg ccggactgat
    26461 tccagttcgg ttgtccggcg gtttgcgtgg tgtccaggtg aatatgtggc tccacaaacg
    26521 gcggtataac taaaccttgt tcggcatcca ggctgttttc agttatgggc atcacgccgg
    26581 attgcgcatc aatggcgctg atttttccgt cctgcagatg aatctgccac agcccctctt
    26641 tgcctggtaa ccgggcgtta ataattgttt gtaaagcgtt attcgacact gttagcctcc
    26701 acatgcgtca ttgctgctgt cgttttacga ttcaaaatcg ggttaaggat cagatagctc
    26761 agcgcgccac ctaataccgc gttgaccgga acaatacccg gtaaccagtg gcctgcggca
    26821 atccccagcg cgaccgccag aatcgccacc caattgacac tcatcatacg cgtggtcgca
    26881 aagtgctcat agcggcgacg gttcatcaga tagtcagcga tgatcacgcc ccctaccgga
    26941 ggaatagctg ccgaaaggaa ggtcagccag ccgacaaaat tgttatacaa ccataatgcg
    27001 cagaccgtac cgataatacc gttaatcacc gaaagggttt tactcgacat accggtaatg
    27061 ttggcgaaac ctaaacccga cgcataaagt gcgttatcgt tggtggtcca gatattcagc
    27121 cccagcacca caatcgcagg cagcagcaga ccctgagcaa tcatcacatc agagatatcc
    27181 gccatcccca gtgccgccgc gcccgctgca ccgaaaataa acatcaacga gttgccgagg
    27241 aaaaaggcca ccatcgccac cagcaccgcc agtttggcat tacgaccaaa ccggacaaag
    27301 tcagcggtga gcgtgcccgc actgataaat gaccccacaa ccagcgccag cgcgacattg
    27361 aaatctaacg gttgtgcggg aacgaccgct tttaatgcgt ccaggccgcc catgccgtta
    27421 acggccaacc acacggaata accgccaaga caggcgatag ccggaactgc aatcaccgaa
    27481 agaaccgtca gcgccgaaat gccaaagaag acggtgacgg tcatcagtaa accggaaacg
    27541 gcaatcagca aattaatatc cagcccggtt gccttgccca ccggaatggc aaacatcgcc
    27601 acgccaacgc caaaccagcc aacctgggtg ccgcccagca gcagtgaagg cagccatgag
    27661 cctttaacac caaaagagaa gcgagcaaga agatgagtgg tcaggccggt ttttgcgcca
    27721 atgtaaccaa gaaatgaagt gtaaataccg aggagaagat taccgatgag gactgcgagg
    27781 aagaaatcat gatagctaag accggttccg agagtgccgc cggtccacat actggcggaa
    27841 aagaaggtta atcccagcat gacgaacgtc aatgctaata cccctttccg cgccgactgc
    27901 gggactggcc cctggctaaa gttgttatct tgcgacacga aattccccct ttttgctgtt
    27961 tgaaacccaa aaaatccgcc gcattctatt catctgaaga taaaaagcaa tcgttttcgt
    28021 ggggaaatat attttttata tgaatgagtg caaaaaggtg ggggaaaaat tatcggtgta
    28081 aaaacagtgg gtaggcataa taagacgcgg taagcgtcgc atcaggcatc ggcgcatggt
    28141 gtcggatgcg gcgcgagcgc cttatccgac ctacggttca ggcctgtagg catgataaga
    28201 cgcgtcagcg tcgcatcagg catctgcgca cggtgtcgga tgcggcgcga gcgccttatc
    28261 cgacctactc ttccatcgcc tggcggatct gatccaacga cgccggatca tcaatggtcg
    28321 tcaggttccc aggatcacgt ccttcgcaaa tcgcctggat cgtgcggcgc agcatttttc
    28381 cagatcgcgt tttcggcaat tgcgagacaa accagacgtg cgccgggcgg ccaaagttgc
    28441 caatctggct gtccaccagc gccataatcg ccttctcttg cgagtgcgcc acctcacggt
    28501 cttccagact gtcgctctct ttcggaatga caaacgccac cgccacctgc cctttcagcg
    28561 catctttcac cccaaccacc gccacttcgg caacgcccgg atgactggag atactctctt
    28621 caatctcgcg cgtccccagc cgatgcccgg caacgttaat cacatcgtca gtgcgcccga
    28681 gaataaagtg ataaccgtca gcatcgcgga tgccccagtc aaaggtggca tacaccggac
    28741 gggaaaacag cgaccagtac gtcttcacaa agcggtcgtc atcgccccag atggtctgaa
    28801 tacaccccgg cggcagcggc ccttccacca ccagcatccc tttctcgttg acgccgcacg
    28861 gttcgccggt gacttcattg agcaactgca cgttatagcc atacatcggc acgccggggc
    28921 tacccagacg cgtcggcctg tcgtccagac cgcgagcaat cgccataatc ggccagccgg
    28981 attcggtctg ccagtaattg tcgatcaccg gcacatccag cgtattgctc acccaactgg
    29041 cggtcggctc gtccagcggt tctccagcca gatagagcac ttccagcgac gagagatcgt
    29101 gtttgcgaat ttcagcggta gggaattttt tcagcacgcg aatggcggtc ggcgctgaga
    29161 acatgcggct aacctgatat ttctcgacaa ttttccacca cacgccgcag tccggccagg
    29221 tcggcaatcc ttcgtaaacg atagtcgcca tccccgccag cagcggcgcg taaacgatat
    29281 acgaatgccc taccacccag ccgatatccg aagcacaaaa gaacacgccg cccgctttgc
    29341 cgccaaaaat gatgtccatc gaggtcgcca gcgccaccgc atatccgccg acatcacgct
    29401 gcacgccttt aggtttgccg gttgtgccgg aggtgtagag aatacaggag gtttcgttgg
    29461 attccagcca cgccaccggc acccgcgcac cgatgtgttg atggcgcaac gacgcgaaat
    29521 cgacatcccg cccgctgacg cgcgccattt tcgccagtcc gcgatccacc agtaaaacgt
    29581 ggcgcggctg atgctgcgcc tgacttatcg catcgtcgag taatttttta tagggaatga
    29641 ttttgccacc gcgcgctccg gcatcagccg agacaatcag caccggttta gcatcatcaa
    29701 ttcgcgccgc cacgctgtgc gaggcaaatc caccaaacac caccgagtga atagcgccaa
    29761 tgcgcgcgca ggccagcagg gtaatatgcg cttcggcaat catcggcata tacaccagca
    29821 cccgatcgcc acgctgcacg cccagtgagc gcagcattga cgccaccgca ttcacttcgt
    29881 catgtaactg gcggaaggta aaggtacgct cttcctctgt ttccgaagag acggcaatca
    29941 atgccagcgc ccctggctgt ttctccagcc agcgatcgat agcgttgtga cacaagttgg
    30001 ttcggccttc acaaaaccaa cgggcaaacg gcgggttgct gtggtctagc gtttgcgtaa
    30061 agggcgtctg ccagtcaata cgccgggcct gctcggccca gaactgctcc ggttcgttaa
    30121 tcgaacgctg ataaaattcg ctaaaagaca taatgctctc ctgttgaact tacgccttac
    30181 cgccgtttac ttaaatgacg tacaggtcga gatactcatt gaccggcatc tgttccaggc
    30241 gagttctgtc gagagaaacc tccagaatgc gctgctgctg gcgagtcggg aactggcgcg
    30301 cgagattgat tttgaattta tcgaccagct tcggaatgcc atcctgacgg cggcgagcat
    30361 gaccaatggg gtactccacc accacttctt caaagcgcgt gccgtcggtg aactcaaggg
    30421 taatggcatt ggcgatggcg cgtttttccg ggtcgtggta gtcagcagta aatgccggat
    30481 cttcaaagca attgatcttc tcgcgcaggg cgtcaatgcg tttatcttgc gcaacgttgt
    30541 cctcgtaatc tgccgccgtt aagcgcccga atagcagcgg gatcgccacc atgtactgaa
    30601 tgcagtgatc gcggtctgcc gggttattga gcggcccttt tttgtcgatg atgcgaatac
    30661 aggcttcgtg ggtgcgaatg gtcacttttt cgatatctgc cgccgttttg cctgctgcct
    30721 gcatctgttc atagagcgtc atcgctgctt caactgccgt ctgggagtgg aactccgccg
    30781 ggaaggagat tttgaacagc acattttcca tgacgtaaga accgtacggg cgctggaagc
    30841 ggaacgattc acctttgaag gagacgtcgt agaagcccca cacaggcgca gtcagggctg
    30901 acgggtaacc catttcgccc gttttcacca tcagtgccag acgtaccgcg cgggaagtgg
    30961 catcgcccgc cgcccaggat ttacgcgtgc cggtgttcgg tgcatgacga taggtgcgca
    31021 gcgactgtcc gtctacccat gccagcgaaa cggcgttgag aatttcctcg cgggtcaggc
    31081 cgagcatttc ggcgaccacg gcggtggaag ccactttcac taacagaacg tggtcgaggc
    31141 cgacgcggtt aaaggagttt tccagcgcga tgcagccctg aatttcatgg gctttgatca
    31201 ttgcggtcag cacctgtttc atggtcaacg gcgctttgcc gctggcgacc gcgttgcgcg
    31261 aaagccagtc tgccgttgcc agaatgccgc cgaggttgtc ggaaggatgc ccccactccg
    31321 ccgccagcca ggtatcgttg aaatcgagcc aacggatcat cgcgccgatg ttaaatgccg
    31381 cctggacggg gtcgagctgg aactgagttc cggggacgcg cacgccgtta ggtacgacgg
    31441 tgccggggac aattggcccc agcagttttt tacaggctgg gtattcgaga gcttcaagac
    31501 cgcagccgag cgtgtcgagc aggcagtaat gtgcggtgtc gtaggctact ttggagctga
    31561 tttcgtagtt catcacgtaa tcgacgatat caacgatttc acgatcaaat tccgggcgga
    31621 tgttgttgat ttgagctgac attgggtacg tttccttatt gttattcgta gaggtttact
    31681 ggcgcttatc cagcgcgaca aactggcggt cttccggtcc aacataattg gcggaaggac
    31741 ggataatttt gttgtcctga cgttgttcga taatgtgcgc cgcccagccg gtgacgcggg
    31801 cgataacaaa cagtggggtg aacatctcgg tgggaacgcc catcatgttg taggaaacag
    31861 cggagaacca gtcgagattg gggaacatct ttttgctctc ccacatcacc gtttccaggc
    31921 gatcggcgat gttgtacatc ttcagcgacc cgccttcctg cgagagctgc ttcgccacac
    31981 gtttgatcac ctgatgacgc gggtcggcga tggtataaac cggatgacca aaaccaatga
    32041 ccacttcttt gttttccacc cgcttgcgga tatcggcttc ggcttcgtcc ggcgtttcgt
    32101 agcgttgctg gacctccagc gacacttcat tcgccccgcc gtgcttcggc ccgcgcagtg
    32161 caccaatcgc gccaataatc gcggaataca tatcagagcc ggtgcccgca atcacccggc
    32221 tggtaaaggt ggaggcgtta aactcgtgtt cggcgtacag caccagcgaa atatgcatcg
    32281 ccttttccca gctttgcgac ggcttttcgc catgcagcag atgcaggaag tgaccgccga
    32341 tggagtcgtc atcggtttcc ggctgaatgc gttcgccgtt gtggctgtag tgataccagt
    32401 agagaagaat cgagctaagt gacgccagca gtttgtcggc aatatcccgc gcgccagaga
    32461 cggtatgccc ctctttttct ggcagcgtgc agccgagcgc ggaaacgccg gtgcgcataa
    32521 catccatcgg gtgtgacgcc gccggtaagg cttccagcac ggtacgcacg ttagccggta
    32581 gaccacgcag ggctttcagt ttcgttttgt aggcggcgag ttcgtcacgg gtcggcagtt
    32641 tgccgtggat cagcagatgc gctacttctt caaattcgca atgttccgcc agatcaagaa
    32701 tatcgtaacc acggtaatgc aagtcattgc cacttttacc cacggtgcag agcgccgtat
    32761 tgcccgccgg aacgccagaa agtgctaccg attttttcgg tttaatgaca tgggtactgt
    32821 tttgcaggat cgttgtgtcg ctcatattgt cctcgtcatt gttatcattt gtagggtcga
    32881 gattgttgtc ggatgcgacg ctatgcgtct tatccgacct acagttcagt tcacctcgta
    32941 ggcctgataa gtcgcgtcag cggcgcatca ggcatcggtt gtcgaataca accaacgggc
    33001 gtttttattt cacctgacca cgggcaaaca ggtcgtcgag cttctcttcg tactggtagt
    33061 agttgatgct ttcgtacagc tcgttgcggg tttgcatggt gtcgatgacg cttttctgcg
    33121 tgccttcctg gcgcaggacg ttgtagacat gttcagcggc gcggttcatg gcgcggaaag
    33181 cagaaagggg atacagcgcc attgcgacat gggcgctgcg taattcgtcg gtggtaaaca
    33241 gcggcgtggc accaaattca gtaatgttgg caaggatcgg cacctgcacc gcatcggcaa
    33301 actggcggta catggcgagt tcggtaattg cttccgggaa cagcatctcg gcacccgctt
    33361 caacataggc ctccgctcgt tcgattgccg catccagccc ctctaccgcc agcgcatcgg
    33421 tgcgcgccat gatcacaaaa tcaggatcgg ttttcgcatc caccgccgcg cagatccgat
    33481 ccaccatctc ttctttcgag acgatcgctt tattcggacg atgaccgcag cgtttcgcac
    33541 caacctgatc ttcaatatgc aatcctgccg caccggcttt aatcattgat ttcaccgtac
    33601 gcgccacgtt aaaggctgaa gaaccaaaac cgatatccgc atccaccagc agcggcagcg
    33661 aacaaacgtc ggtgatacgg cgaatatctg tcagcacgtc atcaagagta gaaataccga
    33721 gatcgggcag ccccagcgac cctgccgcca cgccaccgcc ggagagataa attgcctgat
    33781 atccggcacg ctgcgccaac agcgcatggt tggcgttaat ggtgccaaca atctgcaatg
    33841 gattttcttt agacagtgca gcgcgaaacg ctttacctgg agagtgtaga gacatagccc
    33901 atcctttgtt atcaacttgt tatttgcgtt gataaagaca aagcaagggg tgtgccacaa
    33961 ttgactgaat aaaattaaat gtttaatttc aatttgttat aaataaagtc atgaagtaat
    34021 ccgcaaatat gcgtttcagt taacgtttca ggcaatgttt caccgcgttt cattgcaaca
    34081 attatgaaac aaggctaaac ccaatattcg gtttcttaac tttgcggtgc gctatggcac
    34141 atccaccacg gctgaatgac gacaaaccgg ttatctggac ggtatcggtg acgcgcttat
    34201 tcgagctgtt tcgcgatatc agcctcgagt ttgatcacct ggcgaacatt acccctattc
    34261 agcttggctt tgaaaaagca gtgacctaca tccgcaagaa actggcaaac gaacgctgcg
    34321 acgccatcat cgccgctggc tctaacggcg cgtacctgaa aagccgcctg tcagtgccag
    34381 ttattttgat taaaccgagc ggctacgatg tgttacaggc actggcaaaa gccggaaaac
    34441 tcacctcttc tatcggcgtt gtcacttatc aggaaaccat tccggcactg gtggcgtttc
    34501 aaaaaacctt taatttgcgc ctcgaccaac gtagctacat taccgaagaa gacgcgcgcg
    34561 ggcagattaa cgagctgaaa gccaacggca ccgaagcggt ggtcggcgcg gggctgatta
    34621 ccgatctggc agaagaagcc ggaatgaccg gaatttttat ctattccgcc gccaccgtgc
    34681 gccaggcgtt cagcgatgcg ctggatatga cgcgcatgtc gttacgccat aacactcacg
    34741 atgctacccg taacgccctg cgtactcgtt acgtgctggg cgatatgctc ggtcaatcac
    34801 cacagatgga acaggtgcgg cagactattt tgctgtatgc ccgctccagt gcggcggtgt
    34861 tgattgaggg ggaaacgggg acgggcaaag agctggcggc ccaggcgatt catcgggaat
    34921 attttgcccg ccacgatgcg cgacagggca aaaagtcgca tccgtttgtt gccgtcaact
    34981 gcggagcgat tgccgaatcg ctgctggaag cggagctgtt tggctatgag gaaggggcgt
    35041 ttaccggctc gcgacgcgga ggtcgcgccg ggctgttcga aatcgcccac ggtggcacgc
    35101 tgtttcttga cgagataggc gaaatgccgc tgccgttgca gactcggctg ctgcgggtgc
    35161 tggaagaaaa agaggtcacc cgcgtcggcg ggcatcagcc tgttccggtg gatgtgcggg
    35221 tcattagcgc cactcactgc aatctggaag aagatatgca gcaagggcag tttcgccgtg
    35281 acctgtttta tcggctgagt attttgcgtc tacaactgcc gccgctacgc gagcgggtgg
    35341 cggatattct gccgctggcg gaaagctttt tgaaaatgtc tctggcggca ctctctgtcc
    35401 ctttttctgc cgcattacgt cagggattag agacctgtca gattctatta ttgctctacg
    35461 actggccggg caatattcgt gaactgcgca atatgatgga acgactggcg ttatttttaa
    35521 gtgtggaacc gacgccggat ttaacgccgc aatttttgca gctgctactg ccggaactgg
    35581 cgcgcaagtc ggcaaagact cccattccag gcttgctgac agcacaacag gcactggaga
    35641 aatttaatgg cgataagaca gcagcggcga attatttagg tatcagccga acgacgttct
    35701 ggcggcggct gaaaagctga actgccgaat gcagcgcgaa cgctttatcc ggcataaatt
    35761 tcaatagatg atgatcaccg ataaacttac gcatcgggga tgtttacata ggattcagaa
    35821 ttacttcttc ttataaatat ttgccgtgcc gtggatctta ttgtcagttt gaccagaggt
    35881 cagtaccaac acatcagccc ctttttcatc ggcttttttg atcaaatctt ctttcgcatc
    35941 tgcggtcgac atttcattac tggttgaaat atcacctatt ttttcatatt gcgattcaac
    36001 cttttcaaat tccgctttgg tcattaattc tgcggcatag acattggtaa cggcaaacgc
    36061 taacgcaccg actaacattt tagagattat tttcatatta cggctccaga gttgttatca
    36121 cactatcacc cacccacatt attgggttga ttaataatta gttcagtcag ggcataaacg
    36181 ccagtaaccg gaaataagac actttgtgat ctctctcgca cccgattatt tttggtagaa
    36241 atatgatgaa gttacttcac ttatttatgg atgaaatcac tatggatcct ttgcacgccg
    36301 tttatctgac cgtaggactg ttcgtgatta ctttttttaa tccgggagcc aatctctttg
    36361 tggtagtaca aaccagcctg gcttccggtc gacgcgcagg ggtgctgacc ggactgggcg
    36421 tggcgttggg cgatgcattt tattccgggt tgggtttgtt tggtcttgca acactaatta
    36481 cgcagtgtga ggagattttt tcgcttatca gaatcgtcgg cggcgcttat ctcttatggt
    36541 ttgcgtggtg cagcatgcgc cgccagtcaa caccgcaaat gagcacacta caacaaccga
    36601 ttagcgcccc ctggtatgtc tttttccgcc gtggattaat taccgatctc tctaacccgc
    36661 aaaccgtttt attttttatc agtattttct cagtaacatt acatgccgaa acaccaacat
    36721 gggcacgatt aatggcctgg gcggggattg tgctcgcatc aattatctgg cgagtctttc
    36781 ttagtcaggc gttttctttg cccgcagtgc gtcgtgctta tgggcgtatg caacgcgttg
    36841 ccagttgggt tattggtgca attattggtg tattcgcgct acgcctgatt tacgaagggg
    36901 tgacgcagcg gtgagcagat aatttactct ctacgtaatc aacctgattt gccgaatccc
    36961 aaccatcggc gatgatagcc cccgtaatgc gtggtcctta tttccgataa gtttacttga
    37021 gtctttagct tcccatttca gcccactctc ccctgtgtgg gcttttttca ttgttctgac
    37081 ccgtcctgaa tagccaaaca atggcaatgt tccactttgc agggatgata ataagggatc
    37141 tgattcagaa aagttttgaa aagttggacc gccatcgagg cctcgaaccc cgtaccatac
    37201 aacaatcctg gttgtcgtct gctcttcctg atgagctaat ggcggttttt gatatgagcc
    37261 ttacaacata ttttctgtca ggcttgagtg cgatgcgtaa gatacagaaa agaccccaca
    37321 caaacatgat ggaattatga tttttttcat atgaatgtca acccacacat tcacaaaaca
    37381 tctgtatgag aacatttttt atacaccata tagctgcgaa accactactg gtgctttttt
    37441 aagcgaaact ccgccgttca caacaaaata tcggcgaaaa aggaggggga atgccatttg
    37501 gcatacagat atacagatga aatgatgacc tgctttgcga ttcactctct ggtggaataa
    37561 caacttgcag gtggctctct taaataaaaa taaacgtcgc agataataaa gctctgatta
    37621 aaccatacac tatcagaaat cgctcataac taacgtgtaa agtagtggag ttaatttaac
    37681 aatctgaatt tacgctatag gtttaccact actttgatat tatttcgctc tccttaacta
    37741 aaaagttaag tggagcgaag ttaatatgtt aaaccacagg atgtttatta attttttcag
    37801 tctgttagtg tgcgattatc gataacaaaa cgatatttca catcaccacg cagcattcgc
    37861 tcataggctt cattaatttg atcggcccga atcatctcta tatcagccac gatgccatgt
    37921 tcggcgcaaa aatcgagcat ctcctgagtt tctggaatgc cgccaatcat agaaccagct
    37981 atcgcgcggc gtttcatgat caggttgaaa acttccggcg atttatgcgg tgtcgcaggc
    38041 gcaccaacca gcgtcatggt gccatcgcgc ttcagcaagg tggtaaaatc gtcgagatta
    38101 tgtggcgcag ctactgtatt caaaatgaaa tcgaaactct tgagatgagc cgccatctca
    38161 tcggcattgc gtgagttaac aacttcatcg gcccccaggg cttttgccgc ttcgcgtttt
    38221 gactcagaag tggtaaatgc caccacatgc gcccccatcg cgtgggccag cttgatcccc
    38281 atatgtccca gaccgccgat gccgaccacg cccacttttt tacccggccc ggcctgccag
    38341 tgacgtagcg gcgagtatgt ggtgatccct gcacacaaca aaggagccac cgccgccagg
    38401 tgctcttgcg ggtgacgaat acgcagaaca tatcgctcat gaacgacgat ctgttgtgag
    38461 tagccgccca gagtatggcc cggttcgtcc ggcgtcggcg agttataggt gccggtcatg
    38521 tgatcacagt agttttccag cccgtcttca cactcttcgc aatgtttaca actgtcgaca
    38581 atgcagccga caccaaccag atcgcccggc gcatattttt ctacctgatc accaacagct
    38641 accacgcgcc ccacaatttc atgacccggc acgcaggggt aaaccgtccc cgcccactcc
    38701 gaacggacct ggtggagatc ggaatggcag acgccacagt aagcgatttc gattttgaca
    38761 tcatgcggtc gcggttcacg ccgggtgata tccatcggtt caagtggttg tttagcggaa
    38821 tatgcaccaa cagctttgat cttcattgtg tttactcctg attagctatg tgtatttggc
    38881 gatgcctgat atgtcatcgc ggcaaaacgc gtccattgaa tatagccaat atttaaacca
    38941 cccgatccag ccctaacatg ccgcgagcct gaatgaaaca gagcagcaac gttccccata
    39001 aggccatcgt caggtagggg cttataccga gaatattgaa gccgctttca ataatttgca
    39061 gaagaaacag cgccagcacc ataccaatta tccgcccgct gccgccgtcc ggattaaccc
    39121 cgccgagtac tgccgcgagg attgacacca gcaagtacga ttcgccataa gaggccttag
    39181 ccgagttgag tttcgacatc atcaaaaatg ccgctaccgc acaaagcaag gcggaaatga
    39241 catacaccca tatcagcacc cgacgagtgt taataccgga gtaaagtgtt gctcgttcgt
    39301 tagaaccaat cagataaatg gatttaccga gcggcgtttt ctccagcaat atccacagcc
    39361 ccagcgccac tgcggtaaac aaccacatcg gtagcggaat accgaaccac tgtgcatgat
    39421 tcaaccacag cacccatgag ggataattgg caatggcgct accgcctgtc accagaatat
    39481 ttaccccttt gagcaacgtc atcatcccga gtgttgcaag aattggcgac actcgaatac
    39541 cagcaatcaa tacgccgtta cataaaccga ttatcaccgc cgcgccagcg cccgccagca
    39601 gcgtagcgac aaccgtagca atacccggtg gatactgagt cgccacccac gccatcacca
    39661 gtgaacaggc gttagcagtg gctataattg acaagttaat gccaccgcaa agcatggtca
    39721 ccgccatagc caacgccaga atgcccagca cgggcatttg cgaagcgacg gactggaaat
    39781 tacttatcga ccagaaaatc tccggcatag taatgctgaa ggctgcaaca gttagcacca
    39841 gcaggccgat caagtaaaac tcaacgttgt tacgccacga ttttttcata gcagactccg
    39901 gttctgatgc tgactccacg ccgtggcact aatgctggca acgatgatga tgccggtgat
    39961 caatgtttgc cagtaagacg agactcccaa taaatttagc ccgttttgca tcactgccaa
    40021 caacaccacg ccgagcaaag taccggttaa cgtgccgcgc ccgccgagca aactagtgcc
    40081 gccaagcacc accgcagcca gtactgtcag ctcataaccc agaagagaat cgggggcgac
    40141 agtcatcacc gtccacgact gcactacacc cgcagcacca gacatcaatc ccatatagcc
    40201 gtagacgaaa agttgcagtt tcagcacgct aaagccgatg cgactggctg attcgcggtt
    40261 cccgccaagg gcataaattt tgcgccctac actggtgaaa ttcatgataa acgctgttag
    40321 cagcaccacc gtaatcatgg cgatcagcgg cagaccaagg ccatagtcat agccatcagc
    40381 atcggtgtac ttgaacaaca taaccccctg ctcaaaccac ggcggaaagt cgtacagcca
    40441 cacaccttta ctcaaccata acagcaggcc atagaaaata ttcagcgtcg agatagtgat
    40501 gatgatagac ggcacccgta ggcgattaac cagtaaggcg tttattaagc caagtaaaat
    40561 gccgatgccg cccgccaggg caaacgcgac agcaaagttg ccacccattt tttgcagcaa
    40621 cagcaccatg ccgtattgcg caatgatggt cattgccgga aacgaaatat caatcccacc
    40681 ggaaattaac accacaaaca aaccacaggc gagaatagtc aacatggcat agttattggc
    40741 gagatcgtac aaattaccga atgtcagaaa ctcgtcgctg cgccaggcaa ggccaacaaa
    40801 cagcaccaca atcagtaatg ccagccagaa ttcatggcgt tttttcagtt cagccattta
    40861 ccacctccgc aatcctggcc tgagaactgc tgtcaggcag gaagctatgg gtgatctgac
    40921 ctttttgcat taccaaaatc cggtggcttt gataccacgc ttcttcaatt tcatcgcaga
    40981 tcatcaatac cgcaatgcca tgagccgcca gatcgctgat aatgccgtat attcctgctt
    41041 tgttggcgat atcaacaccg acagtcgggc tatcgagaat caacagtcgt ggcccaatcg
    41101 ccagccattt ggcgatcgat actcgctgcg cgttaccccc ggaaagcgta ttcaccggga
    41161 gatgtgggtc agagactttg atggtcaatg ccttgattag cctgtccacc aggtcacgca
    41221 ctttagcttc actcagaaag ccgaagcgat cttttacctt gtgaaaaaca gtgctaatga
    41281 tgttgtcttc aatagattgc gccatcacca aaccgcgcga catgcgatct tccgaaacat
    41341 aaccaatacc gtggcggatg gcgtcctggt tactatgcag tgtaaccggc tgaccgttga
    41401 tgaggatctc accagcatcc ggtcgggtca taccaaacag actcagacat aattccgtgc
    41461 gccccgcacc aagcaggcca acaatcgaca ccacttcgcc cgcttccacc cgcaagttaa
    41521 tattttgata ttccccatga cgacttaaat tacgcacctc cagcaccggg gtgctggatt
    41581 ttccctgcca caactctcgt acctgatatt cgaactcctg gccggtcatc agaaaactga
    41641 gttgttttgt ggtcatctct gcggccggaa aggtcccgac cagctcacca tctttcagca
    41701 ctgaaatacg gtcggaaact tccatcactt cttccagacg atgactgacg aagaccacgc
    41761 agatcccgcg ttcccgcaat tgatgcacca cctgtaataa cccttgcact tcctgatggg
    41821 tcagcgaagc ggttggttca tccatcacga tcaaccgtgc gtcctgcgcc agtgcacggc
    41881 aaattgccac cagctgacag cgggcaattg aaagctcaga aacgagcgta tccagtggca
    41941 atgtgacgtt aatactggtc attgccgcct gcgccacctc acgcagacga cggcggttaa
    42001 caaacagacc gtggtgatag tgattcacgg cgatattctc ccagacactc aggttaggaa
    42061 ataaagagag gtcctgataa atcacctgaa tcccctgcgc caccgaagcg gcgggtgtca
    42121 gctttgacca gtttttccca ccaagcgtaa tttccgcccc ctcatccggg cgataaacac
    42181 cggacataat tttaatcaac gtcgatttac cacagccgtt ttgcccggca aggcagtgca
    42241 cctcacccga cataaagtcg agagaaacat cgcgcagcgc tcgcgtggca tgaaacgttt
    42301 tgttgatatg acgaagggaa aggaaggttt ccatagcatc ccctggcaat aagaatcggg
    42361 gctttcgccc cgaagaaatg gattaataaa gtgagtcgat attgtctttg ttgaccagta
    42421 ataccttatg gaaacggata attcgcttat ccatatctac gtcggcctta ccgagattct
    42481 gcatttctaa tccaggtttg atctcttcac ccttcagcaa cgtgctggcg acggccgcga
    42541 gtgcgtaacc tgcagaggcc ggatcgtaag taatcccttc ggtgatatca ccacttttaa
    42601 tcagtgatgc cgcctgtgaa gggatcatca tgccatagac tgcgacttta tttttcgccc
    42661 gtttctcttt cactgcacgt cccgcgccaa tcggaccgtt tgaaccaaag gagacaaccg
    42721 ctttcaagtc aggataggtt ttcatcaggt ccagtgtggt acgacgtgag tcatccacac
    42781 tctcggcaac cggcatgcgg cgggtaactt catgcatatc cgggtaatgc tctttctggt
    42841 atttcaccag caagtcagcc cataagttat gctgcggcac ggtcaaacta cccacgtaaa
    42901 tcacatagcc gcccttgccc cccatgcgtt tcgccatatg ctcaacatat tcagcggcaa
    42961 atttttcgtt atcaatgatt tcgatatccc agttagcact tggctgaccg ggagattcgt
    43021 tggtcagaac cacaattccg gcatctcgcg cttttttgaa taccggttcc agcacgttgg
    43081 catcgtttgg cacgatagta attgcattaa ccttacgggc gattaaatcc tcaataattt
    43141 taacctgttg cggagcatca gtacttgaag gccccacctg tgacgcatta acaccaaagg
    43201 ctttacccgc ctcaaccaca ccttcgccca tgcgattaaa ccacggcata ccatcgactt
    43261 tagagatatt caccacgact ttttccgctg cctggagcgg cgcagaaatt agcgcagcgc
    43321 ctaataacag cgaagacacc atattgataa caaaacgttt attcatcatt tcagcctcat
    43381 tgcagggtag tcaggaagtg ttcgcccaaa ttagcgcgct ggagagaata agaattttcg
    43441 aatattttta tgcagaataa acagccctat ttcaggcagc tattaaaaat atggttataa
    43501 atatggacaa aacgggcagc aacaataagt gtgacattca tcacgtaaaa aatagcaaaa
    43561 taaacaaaat tagcgatatt ttatgtgtgt gagtttattt cgcgtagata aatagttatc
    43621 tacacaacgt caataagggc atattcttca aagataacgt ccatttctgg tgaatagctt
    43681 ccttctgcct ggaaaaaacg acggtgttca ttacgccaat attcaagact gaggtcacct
    43741 tcaccttcct tacgggcaag ctccgcggtg acatcagaaa agcgcagtaa atgcaggcca
    43801 atcactctga taacacatac aggctcacca cggctatttt ctacaatctt atactcacca
    43861 atcatcggaa atgcgttgtc ttcaatacat cctgccagcg aaccacagga agctgtttta
    43921 acaccagtcg caatcaacgt tgcgaggtga tcggccaacg ctgcagaatc gccaaaagta
    43981 caaaaggaag catggggata tttttgtaag attttttctt ttatggtcat gagaatattt
    44041 tcgcgattac ggaaaaacaa atcacatctt aattactgtt ggtaaccatc agcaagagta
    44101 tcattggttt ttcgatactg aatctttttt atccgaagca ggtcacattt atggcctgat
    44161 aaataccttt gttaaaactc acttttgtgt atttctctca aataatcccc tggaaattat
    44221 gccgggagat tataaagttc aggatcattc attagcagac cattcccgcc ccactccctg
    44281 gggcttaaat ctcttactat tcaccgttgc aggcgtttca aatgcgcatc aacctgccac
    44341 actcccccac accagttgcc ctttatggaa cgttgcggtt ctcggcgaga tacgcgccac
    44401 cgcctcggcg gaacaggagg cgtccaccag cacaaagctg gcgtcatcct gcgctttggg
    44461 ccagacacgc tcgccttttt cattcagcgg caatacatcg ccagtggcta gaaacagcga
    44521 acgggagagg ttctgttcgt taggacgaat atagagctgc gcgtacagat tggctttttc
    44581 cagcatgtcg cccagaccat aaggcgacca gtggtcgata acgctgtcag tgccagtcat
    44641 gacttttacg cctttgtcgt gcaactgttt gagcggcata tgcagcgtgc caatcggcac
    44701 cgtcgaggca atagaaattt gctgcgccac catccggtgc gccagttcat ctacctgttg
    44761 ttcgttgagc gttgccagcg caaaggcgtg actgatggtc agcttgccct tcagctgtgg
    44821 cgttttctct accgtttcaa ccatataatt gatagctgcc acgcctgccg gagtggtttc
    44881 gtgcaggtga atatcgacgc ctttgtcata gtccagcgca atctggaaca tggtgtcgag
    44941 ggatttttcc atcgcgccat cgacactggt cgggtccagc ccgccgacgt aatgcgcgcc
    45001 cgcctgcatt gcttcacgca ttaaaggttc cgatttcgac agcagcaaac cgtgctgcgg
    45061 gaaggcgaca atttcacact cgaagcccgc ctgacgtcgc gccagcaccg actgcaaatt
    45121 ttgcagattt ttcaggccgg aaaccggttc gatattgcag tggctgcggg caatggtggt
    45181 gccttttgac tgcaataaat cgatcagttt ttccgcccgt tcctgagtat atggttgcag
    45241 ctccggcagc attttctgct cgagtttgat catgtcctgg atggtggtgc ctgccggacg
    45301 attgagcgag cgccacggcc cgccgtaaaa ggttttgtcg agatgaatat gcatgtcgcg
    45361 ggtagtgggc agcatcagct taccgccagc gtcatagtgc ggcagcgtgg cgtccggatg
    45421 ctgcttgttc tcgcgcaggg caacaatttt gccgttctga atctctacgg tctggtgcgc
    45481 cgtgcgggtc tgtactgcca caccgttttc gtagtcaaaa ccggtttcca gcagcacgtt
    45541 gtcgagatag tagtgcgggt cagtgatttt catggtgtta ttcgcgtcgc aatggattgt
    45601 gccagagact gccgcatggg cgagcggcac agaggtacca aacagcgcgg cggcggtgac
    45661 catcttaccg ctctggctca gaaactcacg gcggctattg ttttctttca tcttacgtcc
    45721 ttacgtttta ataatatggg tgcccgtttc accacgcagc gccgcaggca ggcattccgg
    45781 cgtggtgata atcacttctt taccgccctg ttccaggaac gccaggctgg cgatgatttt
    45841 tggcaacatg ctgcccggcg ggaaatgccc ttcctgcata tagcgggtca tggtggcaat
    45901 atccacccga tccagcgcct gctgctgcgg tttgccaaag tggatacaga ctttttcgac
    45961 gccggtggtg atcaccagaa tgtcggcgtg aatttcacgg gccagtagcg cggtagagag
    46021 atctttgtcg ataaccgcgt ccacgctttg gtaatctccc gcttcgctgc gcaccacagg
    46081 aattccaccg ccgcccgcgc caattacgat aaagccttgt tggatcagcg ccttaatggc
    46141 aggtgcctcg acaatacgtt tcggttccgg cgaggcgacc acgcggcgat agccccgtcc
    46201 ggcatcttca acaaaacgcc agtcagggtt tgccttttgt aattcgtcac gctggctttc
    46261 actgaagaat acgccgatag gcttggtggg atgggcaaat cccggatcgt ttttatccac
    46321 ttccacctga gtcaccacgg tgacggcttt tttctcgccg tggcgggcca gccggttgtt
    46381 cagcgcctgc tggatcagat agccaatgcc accttgcgta tccgccacac agtttgccag
    46441 cggcgttaag ggcagccctt cgcgctcgtg agcaatctcc gcgcggcgta aatccagccc
    46501 gacctgcggc ccgttaccgt gggtcagcac aatgtcgtaa tcggaagcca gcatttccag
    46561 caccgtatcg gcgacggctt tcaccgcctc cgcctgatgc tcaatcgact ggctggcgtt
    46621 atctttgata atgctgttgc caccaatggc aacgaccaca agttctttca tatttttatg
    46681 tccgctacag agggtgatgg ggttatgacc aacgccaaat tgcctgatgc gctacgctta
    46741 tcaggcctac agattcttgt gccattcgta gaccggataa ggcgttcacg ccgcatccgg
    46801 caatcgtgac aaatgcctga tgcgctacgc ttatcaggcc tacagattct tgcgccactc
    46861 gtaggccgga taaggcgttc acgccgcatc cggcagagac aacgcgcact ttgcaggtca
    46921 gacgccgtaa cgttcgcacc agccaagaat ggctttctcg aagcaggcga atggcggatg
    46981 cacaatgccc gcgccaatca tgccgacgcc cgcatcttta tgggcgatgg cggtgttgat
    47041 caccggcaga atgccgctgc tgcccacgcg ggtgatgtcg attgccgacg gcacgcccat
    47101 aaaaccgagc agcggaatgg tgacgttggg gttttcgccg agagtgattt cacgcatctg
    47161 acgggagaaa tcgatagcct cttccaccgt gccgcccacc agcgcaacga ttgcaggcgc
    47221 ggtcgccatg gcaaatccgc caatgccgta ggtttcagtg atggcgctgt cgccgatatc
    47281 cagccccgaa tcttccggct tataaccggc aaacatcggg ccgatcacct gctgcgccgg
    47341 gccggtaaac cattgccccg gcaggccgct gacccgcagg ccgaactcga cgccgttacg
    47401 cgccatggtg gtgaccacag tgctgtattc aatgccgtgc gccgcatcca tcgccgcttt
    47461 acacatcgcc atccacgtcg ggccggagaa gtagtcgctg ctggcgacaa actcaaacac
    47521 ttcgcgctgt tgctcgacgg aataacccgc ctgaataatc cccggcgtca gcgcctgaat
    47581 cagcagcgtc gtcccggcgt tattgcggtt atggcattca tcgcccatgt gcagcgcctg
    47641 cgccagcatt aagcgcagat cgatttcgcc gataattttc atcgcgtcgc gcagcattgg
    47701 cccctgcaca tcgcgcatcc agttgaggcg gtcaatcacg ctctggtcgt tagcgcccat
    47761 acgcaaaatc ttcgccatct gctcgctcat attggtgtaa gcgatgttgc cgtaggtttt
    47821 gttttttacg atgtgcataa acatcgaggc cgaggtaaca cccgccatcg aacccacgca
    47881 gtcgtgctcg tggcacggcg agaaggtaat ctccccggag gcagccagtt cagccgcctc
    47941 atcgagatct ttcgccagcc cttcgaacac cagcgctccg gtgaccgcgc ctttcatcgc
    48001 gccacacatt ttttcccagg tgatcggcgg cccggcgtga agaatggttt tcggcgtcat
    48061 gcccggcacc acgttaatcg cctgatcaaa accaatcagc accggatgcg actggataat
    48121 gcgctccagc gcctgctggt tagcggcggc gattttgtcc gccagcgggg aatcggcaat
    48181 gttgtccagc gcctgcacca cctgcatatt gccctgcccc ggcggcgtcc agtcgagttg
    48241 ggtcacttct acatgctgct ttttcagatc atcgctaaac atggcgatac cgacgttaat
    48301 aacgttcaac ggttggctaa acagtgacgg gctcatcagg cttcctcccc tttgcagata
    48361 aattcacgcg ccagcaatcc ggtattggtg ctgctgctgg ccagaatcac tccggcatca
    48421 agcaacatct ggctttgttg ttctaacgat ggcgtatcga gatcggtacc cagcacatag
    48481 gcgagaatga tcaactcgcg cccttcggca gcggcgatcg ctttcgcctc tttgatcgcc
    48541 tcgatggtgg aaccgaccgg atcttcatgc gatccaaacc cgagcacaaa atccatcacg
    48601 atcaccgcca cttctggatc gcgcgcttct tcgatcaacc ggctgatgcg gttggtcggg
    48661 tcaatcattg gatgcggctt gccgttggta aaatcgtcat cgccaaagtc gaggaaggta
    48721 tgtttgacgc tgcggttgat atcttgcagg cggaattcag gatcgggctg aatgttgctg
    48781 tagacatcgc catgtttttc catcaccgca aacatggttt cgtcacacag cgtgccgccg
    48841 cagaacaggc cacgaatgta tttctgctgc ggttgcagac gcgcacgcac atccgcaatc
    48901 aacggctggt tcagggtatg caagtcgaga tgttcttgtt tcataccgga gagcatcacc
    48961 gcttttagtg ccgcttcttt gctgccgcga gcaaactgta gcccctgctc atccactggc
    49021 gtttcgccac gaccgaggaa gcagactacc actggcttac ggcaggcacg tgcgcgttcc
    49081 agcactttgc gggccaccgc aggcgcaggc ggtttggaga taagcacaat gatttcagtt
    49141 tgcggatcgt tttccagcat cccgatagcg tcgagcatca tcaggccgcc gattttctcg
    49201 ctcaggtcgc gcccaccggt gccaatcagt tgcgaaacgc cgccgccaaa ttcatggata
    49261 cggacgctca gttcctggct accggtgccg gatgcgccaa caataccgat gttgccgcga
    49321 cgcacggcgt taccaaagca gagcgccgcg ccgttgataa tcgccgtgcc gcagtctggc
    49381 cccatcatca gcagcccttt ttcgtgggcc agttgtttga gcgccagttc atcttcaact
    49441 gtgacgttat cggaaaacag catcacgtta agatcgtttt gcaacgcctg acgcgcttcg
    49501 cgagcggcaa acagaccgtt gaccgaaatc actgccaggt tactttccgg gatatgcttt
    49561 ttggcgctgg caatagtggc gtaacgcgcc tcgtgcgagc cgctttgcgc tttggtgttg
    49621 aacagttctt caatctccac cagcaactgc tcgttgtctg cgcccgcttt gccgttgatg
    49681 acaatcatca ggtcgccgtt tttcgcctgc tccagctccg gcgtcagcag ccctaaattc
    49741 ttcagcacgc ctttgttcat ttcggtcgcc atcgccacaa atgcctgctc gacgccgtcg
    49801 agtttatttg cacgtgtgga gatagacatc agcgagacag aatcaaaata ggtattcggt
    49861 ttaatgacta ttttaactga cataattccc tccgtaggtt tggctcagcg cacaaccatc
    49921 ggccatgcca tacagcatgt cgcagccgga actggagcca atatgtttaa ttttttctat
    49981 tgcctgagtt gcgtgcccag ggatgtcata aataatgtta tgaataaaac gatgaatatt
    50041 ttcccggcag cgttgttgta ctgcggcttc cagcgttatg gcacttaata acgtggtatt
    50101 atttctgccg cgttgcagac cgagataaaa ttcctctttg tatttttccg ccggatgtcc
    50161 ggaaataaat aaaataagcg ccagacctgt taaataatcg tcggctgatg gcgtaaggcc
    50221 aatccctaag cccatcatac tggcgactgc tgaggcgata ttttgtttat cgtttacggc
    50281 ctgaataact gcccgtcgtt taatatgtaa ttctttattt aacgcctgat aaaacggatt
    50341 atcgccgcaa taattaaaca gggtttcatt ttcccccagg tgctgatgaa taatgtcatg
    50401 ccactgttgc caggcgataa ggtgaaaatt cacagcggcc atttgcaagg ttggggacag
    50461 ccaacgccga caacgactca tctctatatg aagatgttga ccaaccgtaa tgccttgatc
    50521 gtcaaactga accttatcac catgccggaa cagaccatca aagtgagtga gtgccaaccg
    50581 acaactgttt ggcgcattgt cgtactcttc acaaagcagc gtcagcaatt gctggcgggc
    50641 cggaagtgcg atatttaccg ctctggcaaa gacctgttca acctgacctt gcccccgctg
    50701 cgccagaaaa tcagcatccg cagttagcgc ccacacacac cgtctttctg gtgtgttgat
    50761 agcaagcctc ccggtttatg cacctgcggc aatcagtaac tgcgcaatct cttcaaagcc
    50821 tcgttcctgc gccagttcca gcggcgtttt gccatattta tccgtcatgt gcgggctggc
    50881 accgtgttca agcaataact ggacaatcgc ctgctgttta atcccgccat cattaagcac
    50941 aattgcttcc agtagcggcg tccagccaac atggttggtc tggttaacgt taatctccgt
    51001 atgcgccaaa agctctttta caatacttaa atggcctttt tcacaggcag gcgtcaggcc
    51061 gacaccgcca aaacgggtga cgcaattaag atcgggttta gccggtaaaa taatccgtaa
    51121 cagggttaaa tcgccggtca ggcagctaat taaaaaagga tttgaacata tctgatcttg
    51181 cttattaata tccgctccgg cgtcaattaa tgcctgaacg caagcatatt gctgatataa
    51241 acttgccagc gtaattgccg ttttcccctg acgatcgcag atattaatat cgacacccag
    51301 cgcaaggcag gtttttactt tatcgatatc accctgttgc gcagccaata aatagtcggc
    51361 aggtagattt tttatagtca ttcatattcc cctggttttt gtggctgttt tcgatgaatt
    51421 gagaatagca acgataatct gataaaagaa cccgcttaaa tttaattcgc cgtatttcgc
    51481 ctttttgttg aatgatgttc aacaatcttt tcgcagtgtg cggtagtgca atatttgttt
    51541 ctttgacccg ataccctttc actcaatcag gttttatttc tctatagtct gcacatgtaa
    51601 gagtagcttt cgtccatgtc taaccagagg ggaacaagat gaacggactc acggcaacgg
    51661 gtgtcaccgt cggtatttgt gcagggcttt ggcagttagt gtcttcacat gtcgggctct
    51721 ctcagggatg ggaattatta ggaaccattg gcttcgtcgc cttttgcagt ttttatgccg
    51781 caggcggcgg taaatcgggg tttatcagaa gtctggcggt taattattcc gggatggtgt
    51841 gggcgttttt cgccgcgctg gctgccggct ggctggcctc ggtgagtgga ttgtccacgt
    51901 tctgggcgag cgtgattatg actgtaccct tctcggcggt ggtggtctgg caaggacgtt
    51961 tcggtttatt gtcttttatt cccggaggct ttctcggcat gacgctattt tttgccagcg
    52021 ggatgaactg gacggtgaca ttactgggtt ttctggcggg taactgcgtg gggataattt
    52081 cagaatatgg cggacaaaaa ttaagcgaag cgacgactaa acgcgacggg tattaaaagt
    52141 tattcaaaaa tgattatcta tctttaaccg atacctctac agtcatgact ggagaaattc
    52201 atattcgata atggcgggaa tggtttaatg aactcaattt ttaccgaaga gaatctgttg
    52261 gcatttacta ccgctgcgcg ttttggcagt ttcagtaaag ccgcagaaga gttgggttta
    52321 accacttccg ccattagcta caccatcaag cgaatggaaa cagggctgga tgtggtgctg
    52381 ttcactcgca gtacccgaag cattgagtta acggagtccg ggcgttattt tttccgcaaa
    52441 gctaccgacc tgctgaatga ttttcacgcc atcaaacgca gcatcgatac tatttcgcag
    52501 ggtatagaag cacgggtgcg catttgtatt aaccaactgc tttatacgcc caaacacacc
    52561 gcacgcttat tacaggtact gaaaaagcag tttccaacct gccagattac cgtgacgacc
    52621 gaagtgtata acggcgtctg ggatgcgatt attaataatc aggcgaatat tgctatcggc
    52681 gcgcccgaca cactgctgga cggcggcggc attgattaca cggagattgg cgcaattcgc
    52741 tgggcttttg ctatcgcccc ggatcaccca ttagcctttg tgccggaacc catcgccgaa
    52801 agccagctgc gtctctaccc taatattatg gtggaggaca ccgcgcatac gattaataaa
    52861 aaagtgggct ggttgctgca tgggcaggag tcgattctgg tgccagattt caacaccaaa
    52921 tgtcagtgcc agattttagg cgaaggaatt ggttttttgc cggattacat ggtccgcgag
    52981 gcgatggcac aatccctgct ggttacccga caaatccata atccgcgcca ggattcacgc
    53041 atgttactgg cgacccagca ctctgccacc ggtcaggtga cgcagtggat taaaaaacaa
    53101 ttttcgccaa atggcatatt aacggggatc tatcaggatt tactgcatcg ggagaattaa
    53161 tcgctgccaa cctcccgatg cgctgtgaat agcggtttac atcaaccacc tgctttcatt
    53221 acccattcag agataaattt attacccggt actggcggag agtacaaata gccctgcaaa
    53281 aagtgaacgc ctttgccgat cattaaatcc gcctgctcct gggtttctac cccttccgcc
    53341 acgatactca gaccaggctt acgcgccagt tcgacaatat tgtccacaat atgaccggag
    53401 atttcgtcca cgctcgccat ttgcacaaat gacttatcga tcttaataaa atcgaccggg
    53461 aacgcctgca agtaacgata ggtcgcataa cccgtaccaa agtcatccag cgcaaaggta
    53521 atgttgtgct gatgaaggct gtcaaatatc gctctggctt ctggcgttac cggaataggg
    53581 ttacgttccg ttagctcgag aaccagtttg attttatcgt tacctaattt cttaaccagg
    53641 ttcaggcatt ctttttcaaa tcccgcagcc aaaaaacaac ccgccgagac gttgatgcca
    53701 atatggaaat tgtccggcag caaatgtttt accggcatca gaatatccgc agtctgtttc
    53761 atcaactggc gagtcattat gacgataaga ccagatgact ccgccagagg aataaactga
    53821 tccggtggga taattcccgt ttgtggatgt tcccagcgga caagcacctc acagcccgtc
    53881 agtaccccag tctgcgcgca gaaaaccggt tggatccacg gtttgaattc gtggttttcc
    53941 aacgccagac tgatggcttc aggcgtgacg atatggtgac aacggttatc agggatataa
    54001 aaattatttt tatttcccgt gccactgatg acttgcggat gatattgaaa aaagatatcc
    54061 cgccagaagg taatatcact ctggatgcct agtttttcat acagctgctt cttttgatgt
    54121 gaaattgtct ttgcactgcg atttctgtac tgtgagattt ctgtaaccga cattccctcc
    54181 attagcagct tgagtaaaag ccgttctttt tcggacacga taccaggcaa cgtcaaattc
    54241 accgttgccc cgaactcttc caaaaaaaca ctcaaatcat gcgaagccat tgaacacacc
    54301 ttgattaaat cttgtgcgcc agctaaatct gcgagtcttc actctgactg ataaagctgg
    54361 ctgcaagaaa atgacacaga gagccgaatt atctcccgtt cggctctctc tccttttact
    54421 cattattcag atcagaattg ccacttaatg ccaaccattg cagaggtgtc attatatccc
    54481 ctatcggcaa cctgaacgcc cacattcccc cagacattca ggttggcatt taactgtcct
    54541 tccacacccg tttttatctc agcaatattt cgggctccat cctgagtgac gggcacgcca
    54601 tccatactgg tgctgaaatc cttactgtta tgcagccagt tcacttctac aaacggctgg
    54661 aactcgcggg atttaccgcc atccatttta tggtggctct tcagccaggt ttttacgcca
    54721 agtcgtgttt gaacattgcc atcaccgttg ctatgaacga gggttccgtt gctttcgcgg
    54781 tgattatcgg ctttgactcc catccaggta acctgtgctt gcggctgaac ataccattca
    54841 ttacgcgttc cctggctgcc attaaattca gctaatttgt gtttgtatcc agcttccagt
    54901 gaagcggtaa atccttttga tttataggat tcactttgta agtcatcccc tttcactgtg
    54961 ttatcaaacc agctgtactg cgcccaactg tcgagatacg cgccattacg cgactcgtca
    55021 tcggcatacc aggtggcata gaggcctgtg ctatatccgt tcacactcgc ttttgcacga
    55081 taaccagttc gcgaggaaat ggttttgctg tcgctgttgc catatcccgc catgacccca
    55141 acatgccagc tgtcgctgcc gttttggctc cactgcgcga tatcgcctcc cagttgcaga
    55201 acatagcgat tgctttgggt tttcagctgg ccgctgccat cacgccattt attatgacca
    55261 ccttcatggc gcatccacat agtggtttgt ttctgctcac ccgtcaccat gtcggtatag
    55321 tacgtattac ccagacgctc atgcagacgc gtggtgaaca tggtattcgc tgctgcaagg
    55381 ttcgcaatgt agctacccgc ctccggtcgc aggtcattat ccacattcag atccggaccc
    55441 ggcgtaggtg tcgggttagg gttcggctct ggattcgggt ttggctccgg attcggcatc
    55501 gggtctggct ccggctgcag ttcaggagaa tcactgccgc tggtcagata ccagttacca
    55561 ctatttgccc cttgtccacg cgcgagagtg tagtcatatg cccccgcaac gatacgacca
    55621 gcctgaacaa attcgccctc agacttaccg tcaacatgga taagttcgat accgttaagg
    55681 gttttcgcac ctgtaccgcc agcgttattc accgtaacgg cagtcgtgcc gctagtatcg
    55741 ccctcgacaa ccaatttatc ggttactgag tcatcgccat tcagtaccgt attcattact
    55801 agttgaccgt tattaccgtg gtagttcccc ttcaccacta acacattgcc agcttgctgt
    55861 cctgacattc cggtcatcag cgtaccgttg ttggtcagat tgccgcccag ggtgtaattc
    55921 acagacgcag ccgctctttt ttcagcgcta ttattcaacg ccagggtacc gttgttctca
    55981 acgtttgcgc tacccaatcc ctgcgcatga cttaccagga cattagccga attagcaatg
    56041 ttcacattcg ccgtcaggtt gctgttatcg cccgcgatgt ccagcgtgcc gccctgaata
    56101 ttcagctctc cgctccccgt taacgaaccg gttgaagtgc ccccgttggt aaccgtcaga
    56161 ctcccgccgt tcaagctcag cagtgaatcg gcgctgccat tgagcgtgcc cacgatctgg
    56221 ctgtgaccat tcatatccag ttcagtctct gccgccagac gcagttcatg ggtattaccc
    56281 aacacgttat cgttattcaa caaaagcgtc ccactgcgca gatcggtaac accggtatag
    56341 tcgttgtctt tgttagaaag cgatacggtc tgacccttct ggctgctgaa tgccaggtca
    56401 ccactgccgg taatctttgc gctgagatcg gcggcattct cgcttttacc gttagagctc
    56461 agcaccagag catcgctgtc ggtagcgtgt aaatcaagct gggtcaggcc gtaaccgata
    56521 tacagaccgt cattgtttac cccactcgtc agacgatagt cgtaagtgcc gacggccgct
    56581 ttttgcccat tctgctgaat atcacgttgg gcactgctgg agaggatttg cccattttta
    56641 tcctgcaatt gcagattgcc cgcatcgccc agaacttccg caccctgcgc atcaaccaac
    56701 ttaatggtgc tgttaccatc atcgacttca gtgagcgata acgcagagtc aatatcacga
    56761 gatactgagc ggacaacgtc actgtcagaa acctgaatca caccctcgcc gcgcaaatcc
    56821 agcgttttac tcacgttgac tgtcccttct gtcatctgtg caccagttgc aagggcacca
    56881 aaatcaacgg ttccgccgga gaatcccaaa gtgctaatgg agtgagcgcc cttacccact
    56941 gttacctggc ttccctgacc cgcagtgatc ccgcctttct gcagaaactc cgcattcttt
    57001 tccaggttaa atgtcgtgtt ctggaacgcc agtgaaccct ggaaccagtc tgtataagca
    57061 aaatcatcga aggagaattc gtgattcccc atgtttattt caacaatacc tctaccttca
    57121 agatcgttat caaataccca atctttattg gcattgattt gcagtttgcc atcaacatcc
    57181 aggtccgcac ggccgatgtt cttgtcttca gaaataaccg cgacagcacc agaagcgaca
    57241 tcaatatatc cgctgaatgc atagttattg ccggtcagcg ccactgtccc actgtcgata
    57301 cttattgtac cgttgccgga aatcttattg gcaaaggtca tgctatcgtc agccttcagg
    57361 tgtaatttcc cgcggttggt gatatctgca gtgcctatat tttgggtgat ccccaacgcc
    57421 ccatttttct caatcgttac tttgccgcta aaggcagagt tatcaccggt taagtaagtc
    57481 gtgccattga gcgcattgat gtaacccagg ccctcgctgc cactgacaat atccgcggaa
    57541 atagtcgcgt ctttatccgt gtggtttaag gtgattttct tctcggatgt ctttgcccac
    57601 acaaactcaa ttttttcggc atcaataatc cctgccgctt tcgctgcgct tccttcctgt
    57661 gcacctaacg ctaactcaga gttggtgctt aaactaatag tcggcgcaga gattttacca
    57721 ccttccgaaa cagcaagtgt tgtatcggag ttttgatcat aataacccag gctaattttc
    57781 tcactggcat taacttcacc gccgttatta actgacaggt actgattgcc accgtaactg
    57841 ccgtaaacgc ccataaagaa ggttttagaa tttacctggc tgccttcacc gctgacattt
    57901 ccctgcaaat aggtcagacg gctggtgccc ttaacatgga ttttgccgcc gttggagaca
    57961 ttaaaatagc cgcgatcgtg tctcatgcct gagtttgatt catacaaatc atcagcatat
    58021 aactcgctgc cagcattgga gacattcagg aaccccgtac ctttcagcaa aaagttaagt
    58081 gggccattga ttgttacctt tcctccggta atatccaacc caccaccaga agcgagagtc
    58141 agacgcggac gggaagtatc tgtcgcattg attaccagat tggcattgtc agtcaccgta
    58201 atacggtaat cgatgctctg gttctctttg ccaaaatcaa tagttttatc ctagtttacg
    58261 atgatatcat cggcgaatac cagagatgaa tataaactta tcgcgctaat taaaacagcc
    58321 cgggactttt ttccttttac tctgctcccc agctcagagc agacgctcca acagttacgg
    58381 gacctgttcc actttagccg atatatttta ttcatcacaa tctccagcaa tacatccaaa
    58441 taaaaccaag attgcataaa cagcaacgca ttaaatgcca ttccggttac tgttcatcgc
    58501 atataaaaat tcaaaaattt tgcatagcat tggcaaccac atatctgagt atgtagttca
    58561 gagggtaata aaatagcaag ttactactga aaacttattt taagttaaca ctcttaactt
    58621 tatcattaca ttccacccca gtctctaata cctgcgcatt atatgtatcc aggaactcaa
    58681 atcaactcat aatataaaca aaagcaggag aaatacattt aatatacagc cggtaaaagt
    58741 ggt
//
LOCUS       DAPHCV010000011        56846 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.011, whole genome shotgun sequence.
ACCESSION   DAPHCV010000011 DAPHCV010000000
VERSION     DAPHCV010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 56846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.011"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..788
                     /gene="nirC"
                     /locus_tag="RH033_000699"
     CDS             <1..788
                     /gene="nirC"
                     /locus_tag="RH033_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HDV2989931.1"
                     /translation="KCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIILIFTLGNL
                     LDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMWAILPQTW
                     LGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALCNWLVCLA
                     IWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYTLAGIGHN
                     LLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            807..2180
                     /gene="cobA"
                     /locus_tag="RH033_000700"
     CDS             807..2180
                     /gene="cobA"
                     /locus_tag="RH033_000700"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDV2989932.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEYGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            2432..2599
                     /locus_tag="RH033_000701"
     CDS             2432..2599
                     /locus_tag="RH033_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HDV2989933.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            complement(2707..3792)
                     /locus_tag="RH033_000702"
     CDS             complement(2707..3792)
                     /locus_tag="RH033_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDV2989934.1"
                     /translation="MKTFPLQSLTLIEAQQKQFALVDTICRHFPGSEFLTCGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKSGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDRYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGAQALEVLRGLVLAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKLGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETILRILRESIASI"
     gene            complement(3804..5108)
                     /locus_tag="RH033_000703"
     CDS             complement(3804..5108)
                     /locus_tag="RH033_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HDV2989935.1"
                     /translation="MDLYIQIILVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGMSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNILYWLGLFVPA"
     gene            complement(5120..5473)
                     /locus_tag="RH033_000704"
     CDS             complement(5120..5473)
                     /locus_tag="RH033_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HDV2989936.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(5484..6362)
                     /locus_tag="RH033_000705"
     CDS             complement(5484..6362)
                     /locus_tag="RH033_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HDV2989937.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     ARGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPQHVATRSVQELA
                     QEMVDEIEQGIDCTDLKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYGYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            complement(6359..7585)
                     /locus_tag="RH033_000706"
     CDS             complement(6359..7585)
                     /locus_tag="RH033_000706"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDV2989938.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPMLEKMGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALLNADWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEVGVPTVLVGKVADIASNPYGVSWQNLVDSQRIMD
                     ITFNEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLIEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGMVATQLGVLTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(7585..8748)
                     /locus_tag="RH033_000707"
     CDS             complement(7585..8748)
                     /locus_tag="RH033_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HDV2989939.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPLAHQ
                     GHLVQIPCHQVSDAVAQGTDVITVFTLDKAREVSAAAVKAGRVQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVADIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLAEYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEFPVSSAVVLCFRTQIFVTRSDVVLLSGIHRGEPEIVGRYDSLGNSLGA"
     gene            complement(8832..9194)
                     /locus_tag="RH033_000708"
     CDS             complement(8832..9194)
                     /locus_tag="RH033_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDV2989940.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGMM
                     AMTHMASALMRSRRGEEIEPLDAELLAELAQSSHWQAVVQLHQVLLKEFALEVKPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(9211..10116)
                     /locus_tag="RH033_000709"
     CDS             complement(9211..10116)
                     /locus_tag="RH033_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2989941.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVNRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKQALLAHQQRVVSGEQEPSY"
     gene            complement(10407..11411)
                     /gene="trpS"
                     /locus_tag="RH033_000710"
     CDS             complement(10407..11411)
                     /gene="trpS"
                     /locus_tag="RH033_000710"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDV2989942.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(11404..12162)
                     /gene="gph"
                     /locus_tag="RH033_000711"
     CDS             complement(11404..12162)
                     /gene="gph"
                     /locus_tag="RH033_000711"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HDV2989943.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLATAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSLPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(12155..12832)
                     /gene="rpe"
                     /locus_tag="RH033_000712"
     CDS             complement(12155..12832)
                     /gene="rpe"
                     /locus_tag="RH033_000712"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDV2989944.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(12850..13686)
                     /gene="dam"
                     /locus_tag="RH033_000713"
     CDS             complement(12850..13686)
                     /gene="dam"
                     /locus_tag="RH033_000713"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HDV2989945.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(13793..15079)
                     /gene="damX"
                     /locus_tag="RH033_000714"
     CDS             complement(13793..15079)
                     /gene="damX"
                     /locus_tag="RH033_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HDV2989946.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPELEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(15171..16259)
                     /gene="aroB"
                     /locus_tag="RH033_000715"
     CDS             complement(15171..16259)
                     /gene="aroB"
                     /locus_tag="RH033_000715"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDV2989947.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRTGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(16316..16837)
                     /gene="aroK"
                     /locus_tag="RH033_000716"
     CDS             complement(16316..16837)
                     /gene="aroK"
                     /locus_tag="RH033_000716"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HDV2989948.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(17238..18476)
                     /gene="hofQ"
                     /locus_tag="RH033_000717"
     CDS             complement(17238..18476)
                     /gene="hofQ"
                     /locus_tag="RH033_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HDV2989949.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSVAWQNDNIA
                     RQEAEQTRAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KAALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTA
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(18388..18792)
                     /gene="hofP"
                     /locus_tag="RH033_000718"
     CDS             complement(18388..18792)
                     /gene="hofP"
                     /locus_tag="RH033_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HDV2989950.1"
                     /translation="MRVKRWLLAGITLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(18782..19222)
                     /gene="hofO"
                     /locus_tag="RH033_000719"
     CDS             complement(18782..19222)
                     /gene="hofO"
                     /locus_tag="RH033_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HDV2989951.1"
                     /translation="MDMLFDWWFATSPRLRQLCWAVWLLMLVTLIFLSSTHHEESDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVYWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(19206..19745)
                     /gene="hofN"
                     /locus_tag="RH033_000720"
     CDS             complement(19206..19745)
                     /gene="hofN"
                     /locus_tag="RH033_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HDV2989952.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     NAEARVNAVLLQAEQQLARSLQITKPRLLERQQLREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDGHAL"
     gene            complement(19745..20524)
                     /gene="hofM"
                     /locus_tag="RH033_000721"
     CDS             complement(19745..20524)
                     /gene="hofM"
                     /locus_tag="RH033_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HDV2989953.1"
                     /translation="MAFKNWQIGLHLQQQEAVAVAIVRSAKECLLHRWWRLPLENDII
                     KDGRIVDVQRLANTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQMAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGITSAKELAAALSVDPES
                     VALCGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKVY"
     gene            20644..23196
                     /gene="mrcA"
                     /locus_tag="RH033_000722"
     CDS             20644..23196
                     /gene="mrcA"
                     /locus_tag="RH033_000722"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HDV2989954.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVVLSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVDDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRHDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(23361..23921)
                     /gene="nudE"
                     /locus_tag="RH033_000723"
     CDS             complement(23361..23921)
                     /gene="nudE"
                     /locus_tag="RH033_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HDV2989955.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     APDFNEARNVSALFLVREWLKGQGRV"
     gene            24241..26376
                     /gene="igaA"
                     /locus_tag="RH033_000724"
     CDS             24241..26376
                     /gene="igaA"
                     /locus_tag="RH033_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HDV2989956.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            26441..27109
                     /gene="yrfG"
                     /locus_tag="RH033_000725"
     CDS             26441..27109
                     /gene="yrfG"
                     /locus_tag="RH033_000725"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HDV2989957.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            27120..27521
                     /gene="hslR"
                     /locus_tag="RH033_000726"
     CDS             27120..27521
                     /gene="hslR"
                     /locus_tag="RH033_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HDV2989958.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            27546..28424
                     /gene="hslO"
                     /locus_tag="RH033_000727"
     CDS             27546..28424
                     /gene="hslO"
                     /locus_tag="RH033_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDV2989959.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNKQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(28558..30282)
                     /locus_tag="RH033_000728"
     CDS             complement(28558..30282)
                     /locus_tag="RH033_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HDV2989960.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNLSWIVYGV
                     PATVAFSSVLLFSVISFKQKRFWGWLALVFIATLGMSGWLKWQIDGMNPWRAEKALWD
                     FGFYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLIFLVIFLANGLTWLVLL
                     LWSELFKLVGITFFKTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVSLYMLEQSGRYGRAALESLKSDAEYMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LADNVLIAPGSGKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSARKEWDALDMSLLPHKITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            30661..32283
                     /gene="pckA"
                     /locus_tag="RH033_000729"
     CDS             30661..32283
                     /gene="pckA"
                     /locus_tag="RH033_000729"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDV2989961.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            32400..32717
                     /locus_tag="RH033_000730"
     CDS             32400..32717
                     /locus_tag="RH033_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDV2989962.1"
                     /translation="MFTFIELQGFSKRRQLLLPDDEFRAFQELLIQDPTAGDIIAGTG
                     SFRKIRWNRSGMGKRGGIRIIYYNVTRNGRIYLALIYPKNEQDDLTEEQRKALKLLSE
                     KLL"
     gene            32776..33072
                     /locus_tag="RH033_000731"
     CDS             32776..33072
                     /locus_tag="RH033_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV2989963.1"
                     /translation="MKDELFADLLASAEEMVRIEKGEETPEPEHVHTFSEIDVKAIRE
                     ATGLRQQDFAVAVGVSYDLVKSWETKRRQPTGAPRKLLLLLQANPFIINQLKTI"
     gene            complement(33104..34456)
                     /gene="envZ"
                     /locus_tag="RH033_000732"
     CDS             complement(33104..34456)
                     /gene="envZ"
                     /locus_tag="RH033_000732"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HDV2989964.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFVSLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGATKEG
                     "
     gene            complement(34453..35172)
                     /gene="ompR"
                     /locus_tag="RH033_000733"
     CDS             complement(34453..35172)
                     /gene="ompR"
                     /locus_tag="RH033_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HDV2989965.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            35400..35876
                     /gene="greB"
                     /locus_tag="RH033_000734"
     CDS             35400..35876
                     /gene="greB"
                     /locus_tag="RH033_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HDV2989966.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            35973..38294
                     /locus_tag="RH033_000735"
     CDS             35973..38294
                     /locus_tag="RH033_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDV2989967.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDELANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVNGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFEEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNDRPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            38732..38959
                     /gene="feoA"
                     /locus_tag="RH033_000736"
     CDS             38732..38959
                     /gene="feoA"
                     /locus_tag="RH033_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HDV2989968.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            38976..41297
                     /gene="feoB"
                     /locus_tag="RH033_000737"
     CDS             38976..41297
                     /gene="feoB"
                     /locus_tag="RH033_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HDV2989969.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            41297..41533
                     /gene="feoC"
                     /locus_tag="RH033_000738"
     CDS             41297..41533
                     /gene="feoC"
                     /locus_tag="RH033_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HDV2989970.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            41736..42629
                     /gene="rpnA"
                     /locus_tag="RH033_000739"
     CDS             41736..42629
                     /gene="rpnA"
                     /locus_tag="RH033_000739"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HDV2989971.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPNLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAEHSPKHKESLMTIAERLRQEGFQKGIRTNALNIAKTMLDAGVS
                     LEDVLRFTSLTVEDLAEINHQ"
     gene            complement(42849..43619)
                     /gene="bioH"
                     /locus_tag="RH033_000740"
     CDS             complement(42849..43619)
                     /gene="bioH"
                     /locus_tag="RH033_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HDV2989972.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGAMSLADMAEAVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFRHVLVALKQRV"
     gene            43657..44340
                     /gene="gntX"
                     /locus_tag="RH033_000741"
     CDS             43657..44340
                     /gene="gntX"
                     /locus_tag="RH033_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HDV2989973.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALARLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCRWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            44399..44974
                     /gene="nfuA"
                     /locus_tag="RH033_000742"
     CDS             44399..44974
                     /gene="nfuA"
                     /locus_tag="RH033_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HDV2989974.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            45335..46651
                     /gene="gntT"
                     /locus_tag="RH033_000743"
     CDS             45335..46651
                     /gene="gntT"
                     /locus_tag="RH033_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HDV2989975.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(46696..48780)
                     /gene="malQ"
                     /locus_tag="RH033_000744"
     CDS             complement(46696..48780)
                     /gene="malQ"
                     /locus_tag="RH033_000744"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HDV2989976.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLEDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMADPVNIPGTSYQYKNWRRKLSATLEAMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(48790..51183)
                     /gene="malP"
                     /locus_tag="RH033_000745"
     CDS             complement(48790..51183)
                     /gene="malP"
                     /locus_tag="RH033_000745"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HDV2989977.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRGNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPQVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            51806..54511
                     /gene="malT"
                     /locus_tag="RH033_000746"
     CDS             51806..54511
                     /gene="malT"
                     /locus_tag="RH033_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HDV2989978.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLTTRHFLLKSAI
                     LRSMNDALINRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTSDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFESAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(54554..55570)
                     /gene="rtcA"
                     /locus_tag="RH033_000747"
     CDS             complement(54554..55570)
                     /gene="rtcA"
                     /locus_tag="RH033_000747"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HDV2989979.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAATEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSSTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKMGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVALFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFSLVEADGVTR
                     VSIE"
     gene            complement(55574..56800)
                     /gene="rtcB"
                     /locus_tag="RH033_000748"
     CDS             complement(55574..56800)
                     /gene="rtcB"
                     /locus_tag="RH033_000748"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HDV2989980.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKMFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
BASE COUNT        13593 a        15526 c        14324 g        13403 t
ORIGIN      
        1 ctaagtgtgc ggctaacgct gcgcgcattg cacgcctgtc ggcaaataac ccgctcggct
       61 tttgggtcag ctccgccatg gcgggggcgt atgtgggtct tgggatcatc ctgattttca
      121 cgctcggtaa tttgctcgac ccgtccgtac gtcctctggt gatgggcgcg acctttggta
      181 tcgccttaac gctggtcatt attgctggtt ctgaactgtt caccggacac accatgttcc
      241 tcacctttgg ggtgaaagcg ggcaccatca gccacgggca aatgtgggca atcctgccgc
      301 aaacctggct gggtaacctg gtcggttccg tcttcgttgc catgctttat agctggggcg
      361 gcggtagcct gctgccggtc gataccagca tcgttcactc cgtcgcgctg gctaaaacca
      421 ctgcaccggc aatggtactg ttcttcaaag gtgcattgtg taactggctg gtttgtctgg
      481 ctatctggat ggcgctgcgc actgaagggg cggcgaaatt tatcgctatc tggtggtgtt
      541 tgctggcatt tatcgcgtcc ggctacgagc actccatcgc taacatgacg ctgttcgcac
      601 tctcctggtt cggcaaccac agcgaagcct acacgctggc gggcattggt cataacctgc
      661 tgtgggtgac gctgggtaat actttatcgg gtgccgtatt catgggattg ggttattggt
      721 atgctacgcc gaaagcgaat cgtccggttg cggacaaatt taatcaaact gaaacggctg
      781 ccggttaatt actaaggggt ttttacgtgg atcatttgcc tatattttgc caattacgcg
      841 atcgcgactg tctgattgtc ggcggtggtg atgtcgcgga acgcaaagca aggttgctgt
      901 tagacgcagg cgctcgctta acggtgaatg cattagcgtt tattccacag ttcaccgcat
      961 gggcagatgc aggcatgtta accctcgtcg aagggccatt tgatgaaagc cttctcgaca
     1021 cctgctggct ggcgattgca gcgacggatg atgacgcgct taaccagcgc gtcagcgaag
     1081 ccgctgaagc tcgtcgcatc ttctgtaacg tggtcgatgc gccgaaagcc gccagcttta
     1141 ttatgccgtc gattattgac cgctcaccgc tcatggtagc ggtatcctct ggcggcacct
     1201 ctccggttct ggcgcgcctg ttgcgcgaaa aactcgaatc actgctgccg ttacatctgg
     1261 gccaggtagc gaaatacgcc gggcaattac gcgggcgagt aaaacagcag ttcgccacga
     1321 tgggtgagcg tcgccgtttc tgggaaaaac tgttcgttaa cgaccgcctg gcgcagtcgc
     1381 tggccaacaa cgatcagaaa gccattactg aaaccaccga acagttaatc aatgaaccgc
     1441 tcgaccatcg cggtgaagtg gtgctggttg gtgcaggtcc gggcgatgcc gggctgctga
     1501 cactgaaagg tctgcaacaa attcagcagg cagatgtggt ggtctacgac cgtctggttt
     1561 ctgacgatat tatgaatctg gtacgccgcg atgctgatcg cgttttcgtc ggcaaacgcg
     1621 cgggatacca ctgcgtaccg caggaagaga ttaaccagat cctgctgcgg gaagcgcaaa
     1681 aaggtaaacg cgtggtgcgt ctgaaaggcg gcgatccgtt tattttcggg cgtggcggag
     1741 aagagctgga aacactgtgc aacgcgggta ttccattctc ggtggttccg ggtattaccg
     1801 cagcttctgg ttgctctgcc tattcgggta ttccgctcac gcatcgcgat tatgcccaga
     1861 gcgtacgctt aattactgga cacttaaaaa ccgggggcga gctggactgg gaaaacctgg
     1921 cggcagaaaa acagacgctg gtgttttata tggggctgaa tcaggctgca actattcagc
     1981 aaaagctgat tgaatacggt atgcctggtg aaatgccggt ggcaattgtc gaaaacggaa
     2041 cggcagtcac gcagcgcgtg attgacggta cgctcacgca gctgggcgaa ctggcgcagc
     2101 aaatgaacag tccatcgcta attattattg gtcgggttgt tggcctgcgc gataaattga
     2161 actggttctc taaccattaa tttaaccagg gccagagaag tctggccttt ttcgtttaca
     2221 actctcttta tatattcagc agataaaaac ataccccctt aataaatatt gtctgctaat
     2281 gggttatatt caccgttaca gcgactgaca aaattaaatc aatcgctcac tgacgctaat
     2341 tgaatattcc tgtatcgttc ttaatgagca gtctgcttat attcatataa tcaatgaata
     2401 ttaattaata tataatacat agggaatgta aatgaacaaa tttattaaag ttgcactggt
     2461 aggcgcagta ctggctacgt taactgcatg tactggtcat attgaaaacc gtgataagaa
     2521 ctgctcttac gactacctgc tgcacccggc aatttctatt tctaaaatca ttggcggttg
     2581 cggtccgact gcacagtaag ccattgacag aaatcggcta acgccggttt aattgcctga
     2641 cgccctcgag attacccgag ggcgtatttg tttctgcatt cttttcaaaa atatcgttta
     2701 caacgattaa atgctggcaa tactttcacg caagatccgc aatatcgtct cttcaccgct
     2761 gcgatttggg ttaatgcgaa tcgcgcaatg ttctaactgc ggattcgcct ggcgaaacgt
     2821 tccggaaagt cgataaaaga gcggcgggat ttcatatttc gactctgcac caaccgggta
     2881 aggcaaggca ccgagtttct gcgcctcttc cagcactctg gcggcaatcg gctgatgaaa
     2941 ctctacaatc aataccttcg actgtgcatt ggcaatgacg gcgcttttca cttccgccac
     3001 cgcgccgccg ttaagcaaag ccagcaaccg ttcagatacc cccgcctgca ccgcgtgcat
     3061 cactggcgcg agcaccagac cacgcaacac ctctaatgcc tgcgccccct gaatctgact
     3121 gccgccggaa tagagcgtgg cgcgaatccg gctaataaca tcggcatcgc ctaccaccgc
     3181 accaacgccc tctggcccaa acagcttgaa gcaggaaaat gtcgacacat tcgccccgca
     3241 ttcgcagccg attcgcgcta ccttcatcac cgcatagttg tcatcggtta acgctggaac
     3301 acctgccgcg cgcaacgttg ccagcacgtc tgccagcaca tagcggtcct gcggctgctg
     3361 gcgcgtatgc tgcaccagcg cggcatcagg ttgttgctcg tcaacaacct gcttcagtgc
     3421 cgacagatca ttgaaatcag ccgtaatgag cgttagcccc atctgctcaa taataacccg
     3481 tgtcgtcggg taaacaggcg cgtcatgcac cagaagacgc tgccccgatt tgagcaaagc
     3541 cgccagtgcc gcacggatcg cgccagtacc cgcgccctgt accagcgccg cggcctgtgc
     3601 gtgaaacgcg tcagccagca cctgttccac acgctgggta atgcggggct gatttagccc
     3661 cggcgttaag cccaaatcac cgcaggtaag aaactcgctg ccaggaaaat gacggcaaat
     3721 cgtgtccacc agcgcaaact gcttttgctg cgcctcaata agcgtcaggc tttgcagagg
     3781 aaacgtcttc ataggtgcct tccttaagcc ggaacgaaca ggccaagcca gtaaagaata
     3841 ttcagcagga tgccggtgat cattactgct acgaccggtg ccgccatttt ctgtaccgga
     3901 cggcccaggg attcgttgag gaagtaaatg gcaaccgcga tagagaatcc ggtatagccc
     3961 gccattttga ttgctgcaaa gatcgaaccg accagcaacg ccacttccat cagcatattc
     4021 atggcgttac ggatgttatc cgacgcatta cgtaccgacg ggtagcgccc caaccatttg
     4081 ccgatcgaac gaagcagcaa gacttccgcc gaaataacca ctgcgcctaa taccgctgca
     4141 accatcggat tcggcgagag atagcccacc gcataaacaa aggtaaagcc cgcaactgca
     4201 tacacaccgg tggctaacgc ggtggtggca atcaacggca caaaacccag cccgcgcata
     4261 aattctgcca gtgccgcctg gttgattagt gtttgcgatt gttccggcgt tacgcctgcg
     4321 gagtacgctt tctccagtgt gaagatcgac acttcactac cggcgaaaat cttcatgctg
     4381 gcaacggcag caatcaatgc acccacgatg gcgatatacg gtaagttttt gataatccgt
     4441 gatgtacgct cttcaaacac cgacataccg ctggcatcga tatcattttc atcacgatgg
     4501 cgcaggtcat gagttattgc gatccccagc agcatcacca tgccaataaa gatttcgatg
     4561 gattcagggt taagatgtgg gaaatagcgc acgacgacca cacgggtcat cagtaccacc
     4621 acggcggcga tcagactttg cttccagcca aactggtaga aaatcgccac cagagggaac
     4681 agtgcaaaag ctgaaaccac cggcgagctt aattccccca gactacctaa tacatccacc
     4741 ggtagcgcgg tcagcagctg gtttactggc aacagacagg tgaggatcaa cacgccccag
     4801 atagcgccaa gaccaaacgc catcaggctg tttatcgcca gtacgccgag gatatcggta
     4861 ggaagaaaga gtaaccatgc gttgagcagc ccggttttca gggtaaacga gatccccacc
     4921 gaggccacaa aaccaatgct cagaccaaaa gcgatactcc ccgcctcgcg acggttcata
     4981 tagccttcaa tcagttgtgg caggatcggg cggataccgt catgaaaaac ggctgccgag
     5041 cgatgcgcca gaagcgatgt catacccgtc aggcacgcca ccaggataat ctgaatatac
     5101 agatccatag tcgtgccttt tatttcagat ggttaatcag catcggaatc gcgtgttcga
     5161 cgtgctcaac ggaaaggcca aacgccactt tcccttcggc gatcattttg gcgatatgtt
     5221 cgtctttcgc tttaatgcct ggtttggcaa tggtgcaact tttgttatag ccaatcaccg
     5281 cgatggcaat cgacaatgcc gcgcccgcac cggtattaca cgcgccgatg tagtaatcca
     5341 gttgcccgga tttcactttc attgccgctt ccatgtcgtt gtgaatgaaa acttcaaaac
     5401 agccaggagc cgtcgcttca atggtttttt taatctgctc acgctgtaag cctgcaacac
     5461 caatcttttt catgagtctg tccttattgg aaaaattgag agggagtttc acgtaacatc
     5521 acatccacat cggcctgact gaatcctgac tggcgcaatt gcggaataaa ggtggtcaat
     5581 aaatagccat agccataacc accattggct tttaaatggg agcggcgcgt aatatccatc
     5641 gacagcatga cgcggttcag caaacctcgg tcacgtagcg cgtggagcat cgcaatgcgc
     5701 ttttcgtccg ggtagtaact gttcttgccg atggtgtcga actgcacata cgcgccgaga
     5761 tcgatcatct tcaaaatgtt gtcgaggttg tctttcagat cgcagtgacc aacggtgacg
     5821 cgcgaaagat caactccgtg ggcttgcagt agcgccagtt gttccagccc catggtgcta
     5881 aaagaggtat gcgtggagat cgggcgtccg gtctggttat gtgccagcgc ggcggcaata
     5941 aacaccttct cttccagcgg cgtaatcttc ccttcgctgg agccgatctc cgcgatgatc
     6001 ccggctttca ggtcggtgca atcgataccc tgttcaattt catcgaccat ctcctgcgcc
     6061 agttcctgca cgctgcgggt cgccacatgt tgcgggaaaa acgcgtcctg gtaataaccg
     6121 gtacaggcca ccacgttgat gccggtttcg cgcattacat cgagcataaa ttgcgcattg
     6181 cgccccatgt aacggttagt catctcaatc acattacgca cgccccgggc catcaggtcg
     6241 ttcatctcct ggcaaatgaa cgcatactga tcaaggcggc agtccacgtt gtttttaaag
     6301 ccggagagat caatatgcag atgctcatgg gcgagggtgt aacccgtcgg atcaaaactc
     6361 atagggtgtc tcctgcaaac cggagggagg aaagaaaaga gcgaccattt tgtggcggtg
     6421 gcgcgcggaa aaattcacat acggtagccc ccacatcaga aagcgtggtg agcacaccaa
     6481 gctgcgtagc gaccatccct tgctgataaa ccagcactgg caccacttcg cgggtatggt
     6541 ggctgtgacc aatggtcgga tcgttaccgt gatccgccat cacgaccagg caatcatctg
     6601 gctgcatcgc ctcaataagc cgggcaaggt tacggtcaac gacctgcaaa cgttccgcat
     6661 aacgtgcgac gtcttctgca tgaccagcga ggtcggtttc ctggatattg gtacaaataa
     6721 acgccgtcgg atgggtgtta aattcgttga aggtgatatc cataatccgc tggctatcca
     6781 ccaggttttg ccagctcacg ccataaggat tactggcaat atccgccact ttccccacca
     6841 gcacggttgg tacgcccact tcatacagtt tttgtggcac ctgcactttt tcatcgacgc
     6901 cgtagcccat atgcacgacc tggaagccgt tgtcataagc gccagaacgc ggcgcattga
     6961 tgccaataaa gcgcccttct ttgctttctg cggcatcgag gatgcgctgg ctgtcggtta
     7021 acaggccacc aaatgtaatg acccgaccga cctgtacctg ctcacgcacg atacgaccaa
     7081 ttttgattgc gtcgtcaaaa gagatcacag agagattggc ggtgatgtta tagacctgac
     7141 ctaaatccgc ctcgagatta tcgccaatcg caaccgcctg attgacccac agaaattgca
     7201 gatcatcgcc acggcgctcc acctgccagt cagcgttaag taatgcttgc tcaacacggt
     7261 caatcacatc gcgaaagggc atccgtagcg gcggtaacgg gcgcgtgcct aaaatttcct
     7321 gatgccccat aaaggtatcg ccaccttcat gttgcagctc tgccacgccc caggttgcgg
     7381 agtctgacgg ctgcatatct ccaggcgcat aacctaatgc attgattagc cccattttct
     7441 ccagcattgg tagctgcaaa tgcggcaact ggctcaggat gtgaccacag gtatttgccc
     7501 cggcatcctg cggacgcacc aacgtgacat ctttcattgc ccctacgcca aagctatcaa
     7561 tcactaacac cacaaatcgc gccattacgc ccccagagag tttccaagac tgtcataacg
     7621 accgacgatt tctggttcgc cacggtgaat accggacagc aacaccacat cgctacgggt
     7681 gacaaaaatc tgcgtgcgaa aacagagcac tactgcactg cttaccggaa actcgcctgc
     7741 cagcggcagg gtgtagtcga tactgctgtc atccacagtt ttgagattgg tttcagtaat
     7801 cttttgattt tctggcgtaa acaccagcgc atgttgcgcg tgaccacgac ggtagtaacc
     7861 gccgccgtag cagtagctgt cgccacgaaa atgatgggag atttcactta accagagcat
     7921 cgcgatacgt tcaggctgat cgccctgctg gtttgccgga atcgtccccg tcagcgcatg
     7981 accgggttcg gcatgagtca caccgtattc cgccagcaat ggcagcgaag tgcagctggt
     8041 cgcagaaggt gcgttcagtt gctcaatcgc aataccagat ttcgccagtt gatcccgtgc
     8101 ctgtatcagc gtgtgaagat tcggtgtcgg caaaactttc ccggcagcct catcccaaag
     8161 caggcaaggg aaatgggtta gtccggctaa atgcagcccc ggcagtttct taatatcagc
     8221 aaccacctcg tgcagcgaat gctgtacaaa accgctctcc tgccccggat aaagaaaatc
     8281 atcgtcgcta taaactttga gcagcacaga ctgcacacgt ccggccttca ccgctgctgc
     8341 agaaacttcc cgcgctttat cgagagtaaa cacggtgatg acgtcagttc cctgtgcaac
     8401 ggcgtcagaa acctggtgac aagggatttg caccagatgc ccctgatgcg ccaacggcag
     8461 accggcgcgg cgcatgaccc gcgcctcttt gtaatccacc gccacaatgc cgctgtagcc
     8521 cagtgccaga agtttttccg ccagccacgg attacggcca aactgcttgg tcatcagata
     8581 cagttcaatg ccgtaaagtc gcgccgtctc aatcagccgc ttaccgtttt ccagtatctg
     8641 atccacatcg atcacccagc tgtccggggc gatcttgccc tgctgccaca aacttagtgc
     8701 ggcggaaatc agcgccgggt tctggcgttt caatgcttct acaaacatct tgttgcctcg
     8761 tttctgttcc ttgaattaaa tattcatttt tctgaatatt tagattatgc gacgtggatt
     8821 tgtcgggagc ttcaaacctc ttcgttagca gccatccata atccataaag attagccagc
     8881 aaatagcctt cttcacacgg cttaacttcc agtgcgaatt ccttcaacaa cacctgatgc
     8941 aattgcacaa cggcttgcca gtggctggat tgcgccagtt ctgccagcaa ttcggcgtcc
     9001 agcggctcta tttcttcacc acggcgactg cgcatcagtg cgctcgccat atgtgtcatc
     9061 gccatcattc cttgctcgct gcgcaccggt agatgacact ctgtttccag tacgttgacg
     9121 acctgcatca tgcctttgca gatgtcctta tcaataacgc ctgcctcaca aagcaggttg
     9181 agtctggttt ccatttcaga tgcctttcgt ttaataactc ggttcctgtt ccccactgac
     9241 cactcgttgt tgatgggcaa gcagggcctg tttatcaaca actgcacgca gcaattgttg
     9301 cattgggtaa tcatcgactc gcgtcaaaac tacggcttcg gtggcctgta aaaatcgcgg
     9361 atcgtctgtg agcggcgtcg cttctaatcc cagcatggtc agttcgtttt ccgccaccac
     9421 gttccagatc accgcatcaa catcgccttt aacaatgcgt tgtaaactct cgtgataaga
     9481 gagatcgact cgttccacat cactatcgcc aaaaaagaca tcggtcatga ttttctgatc
     9541 cgccgaacgg ttatccagcc ccacgcgatt cacgtttgcg gactcgcctt tacggcaaat
     9601 caactggtgc tcgccaacgt aggtgtgcgg ccccaactcc agcgcgaggc ataacccttt
     9661 ttgcgtgaga taactttccg ccgccagtcg ggaaacaacc gccatgtcat acacgccgtt
     9721 aagtaaacac tccacgcgaa tatccgcgcc gcgcatgtgc gcatagtaaa aaggaatacc
     9781 atcaaactga gctttcaatc cgctcgccag gccttcgtac aaacgggtat agggcaaggg
     9841 catcgcgcac accacattgt tgatatccac atgactcagc aatgctttgt tatccatttc
     9901 caccagataa ctgccattac gcccacggcg ttctatccgt atcgctccgc ttgattccag
     9961 cgttttcaac gcggcctgcg tcaggccaac ggatgaacgg cattcagttg ccagttcatc
    10021 tatggttttc agtcgattac cgcacttttc acccaacaaa taacgggcca gtgtcgtgat
    10081 gacgacgcct tcttttttga taaacgttcg acgcatagta aattttcaat aaagtgaata
    10141 tttatatctt cataaaaatg aagataagtg gcaactgcga gatgtggaaa cggcgaggcg
    10201 cttcacattt tttcggatta ttgcaaaaag aggataaaaa aaccgggttt ccccggtttc
    10261 agagtgatga taaaagcaaa attgcctgat gcgctacgct tatcaggcct acatttcctt
    10321 gcaatatgtg cattactttg taggccggat aaggcgttca cgccgcatcc ggcatgaaca
    10381 aagcgcaatt tgccagcaat agtgaattac ggcttcgcca caaaaccaat cgcttcgtac
    10441 accgctttca gcgtacgaga agcgtgtgca ctggcttttt ccgcgccatc tttcatcacc
    10501 tgttgcagga aggcttcatc gttgcggaaa cggtgatagc gttcctgcaa ttcagtcagc
    10561 ataccggaaa cggcatcagc cacttcacct ttcagatgac catacatctt gccttcgaac
    10621 tgtttttcca gttctgggat gctctggccc gttaccgcag aaaggatatc tagcaggttg
    10681 gaaacgcccg ctttgttctg cacatcgtag cgaactaccg gcggctcgtc ggagtcggtg
    10741 accgcgcgtt tgattttctt caccaccgat ttcggatctt ccagcaggcc gataacgtta
    10801 ttgcggttat cgtcagactt ggacatcttt ttggtcggct ccagcagcga cattacgcgc
    10861 gcgccagatt tcggaataaa cggctccggc actttaaaga tctcgccata cagcgcgttg
    10921 aaacgctggg cgatatcgcg gctcagttcc aggtgctgtt tctggtcttc acccaccggt
    10981 accagattag tttgatacag caggatgtcc gcagccatca gcactggata gtcaaacaga
    11041 ccagcattga tgttctcggc ataacgcgca gatttatctt taaactgcgt catgcggctc
    11101 agttcgccga agtaggtata gcagttcagt gcccagccta actgcgcatg ttccggcacg
    11161 tgggactgaa caaaaatggt gcttttctca ggatcgatac cacaagccag atacaaggcc
    11221 agcgtatcca gcgtcgcttt acgcagcttc tgtgcatcct ggcgcacggt gatcgcgtgt
    11281 tggtcaacga tacagtaaat gcaatggtag tcatcctgca tgtttaccca ctgacgcagc
    11341 gcacccatgt agttaccaat ggtcaattca cctgagggct gtgcgccact aaaaacgatg
    11401 ggcttagtca tttttcgatt cctgattttc gctatgcgga agccctaatg cgggcagaag
    11461 gtcatttata gactgataaa ttacatcagg caggctgaga tcgatagcct cgccgtagtt
    11521 atatccgtag gttaagccaa ctgatgggca acctgccgct tttgctgcct gaatatcatt
    11581 gcgtgagtcg ccaacaaaca gcatctgttg tggggcaatt cccatccgct cagccaccag
    11641 taacagcggg tccggatgcg gttttttgtt ttgcacatca tcaccaccaa tcaccacgct
    11701 gaagtattta gcgatatcta aggcttccag cagcggcgcg acgaacggcg tcggtttgtt
    11761 ggtgaccagg cccaacggca ggcctttagc ctgcaacgca cccaacgtat cggcaacatg
    11821 cgggaacaaa aacgtcccct cttcggcaac ctcgccatag tagcgatcga acagtttacg
    11881 cagaatacgt acttgttctt ctgccggaat gtcgtcatca acgggcggtt tacccatcgt
    11941 tttacgcaga gtcgcacgtt cctgacgcgc ccaggtcaat gcgcgctcca tcagaacatc
    12001 tgcgccgtta ccaatccagg ttataacgcg ttcttcacct gcgacgggca actccagcgc
    12061 ataaagcgcc atatctactg cggtagccaa gccaggagca ctgtccacca gcgtaccatc
    12121 gagatcaaaa gcgacgccgc gaatatcttc aaacttattc atgacttacc tttgccagtt
    12181 cactgcgcat ttcatcaatg acttttttgt agtctggctg gtcgaagatt gccgaaccgg
    12241 cgacgaacat atccgcgccc gccgcagcga tttcgccaat gttgttcacc ttcacgccac
    12301 cgtccacttc cagacgaatg tcaaagccag actcgtcgat acgacgacgt acttcgcgca
    12361 gtttatccag tgtttgagga atgaaagact gaccgccgaa accaggattg acggacatca
    12421 gcaggatcac atccagctta tccatcacgt aatccagata gctcagcggt gtcgccgggt
    12481 taaataccag acccgcttta cagccatttt ctttaatcag ttgcaacgtg cggtcaacat
    12541 gctcggaggc ttctggatga aaggtaatga tgctggcacc agcggcagcg aaatcaggca
    12601 caatgcgatc gacgggtttc accatcaggt gtacgtcgat aggggcggta atgccgtagt
    12661 tacgcaagga tttcagcacc attggcccaa tcgtcagatt gggaacatag tggttatcca
    12721 tgacgtcaaa atgcacgaca tcagcgccag ctgccagggc ttttgcggta tcttcaccca
    12781 ggcgggcaaa atcagccgac agaattgagg gggcaatcaa atactgtttc atccgcttct
    12841 ccttgagaat tattttttcg cgggtgaaac gactcctggt ttgtacaaag ccagcagttc
    12901 gtccaccttt ttacgtgtgc cgccgttgct gcttatactg cgtcgaactt tgacgacatg
    12961 caattttgcg cgctgatacc actcacgcgt taacatcgta tcgtgattgg agatcagcac
    13021 tggaatatgg cgatcaacca gaccttcggc gatctctgcc agatgcgctt gttgttcaag
    13081 cgtaaaactg tttgtgtgat acgccgtaaa gttggcggtc gcagacagcg gcgcataagg
    13141 cggatcgcaa tagacgacgg atgcatcatc tgcgcgcgcc atgctatcgg cgtaagactc
    13201 acagtagaaa aaggcattct gcgctttttc agcgaagtga tacaactctg cttccgggaa
    13261 atagggtttt ttgtagcggc caaacggcac gttaaactca ccgcgcagat tgtaacgaca
    13321 cagaccgttg taaccgtagc ggttcaaata taaaaacagt accgcccgac ggaacggatc
    13381 ctggcttttg ttgaactctt cgcggaactg atagtaaacc tcggcgcaat ttgtttcggg
    13441 aacaaacagc tcgcgtgcgg cctgtacgta ctcatcagta cgcatcttca caatgttata
    13501 gagactgatc aggtcgctat tgatatcggc aaggatataa cgagaaaagt cggtgttgag
    13561 aaacaccgac ccggcaccta caaaaggctc aaccagacat tcgcccttgg gcaaatgccg
    13621 tttaatatca tcaagcaggg gatacttgcc ccctgcccac ttcaaaaaag cgcgattttt
    13681 cttcatgctg actaactaat tacaccttct ccggctgtgg agaaagctcc gacagcatcc
    13741 agcgcttcag gcaactcccg caacgataaa ccattgcggg agataacctt gattacttca
    13801 gatcggcctg tacctgacgc agtggtttcg cccacgggtt tttggcctgg acatcggctg
    13861 gcaaggtaga taccgctttt ttcgcctctt ctttcgaagc gtacacgcca gaaaccagga
    13921 cataccacgg ctgaccatta cgcgtcgttt catagacaac gtagtttttc agattctctt
    13981 tcttcgccca accgttcagg ttgtcgtagt tagaggaact gctcagctgc agagtgtaat
    14041 ggctggacgg tgccgatttc aacgaaccaa cattacctgc ggtcttccct ccagcggctg
    14101 gtgttgccgt ggtttgcgcc ggggatgccg tctgtactgg agccgtagtc gccgtctctt
    14161 ttggtgctgg cgtagaagtc gcagccggtg ccttcgtgct tgccacagga gcagctggtt
    14221 cagtacgctt cggcgtctgt gctaccggct tcggctccgt tttcacggtt gcttgcggtt
    14281 ttttcggttc aatcaccgcc tgctggcgag ctggacgcgt agtggccgga cgttcagcgg
    14341 tttgcgtttt cgccgtgtca cgcgatgcat tgccattgcg aacaggcgcg accgtcgcgg
    14401 gttcagtcgg caacgtggaa ttgaccgcca cattattcaa ctgttgctga ttttgtggct
    14461 gggtcagcgc attgttcagg tcaccctgca cttcaacacg ttgttgacca tccgttgccg
    14521 ccggggtttg cccttgagtc ggcgtagaag agatcggcgg tagagaaaca tcctgctgag
    14581 tattttccgc agacgtggtt cccggcgctg gctgcacgcc attcgcctga tcggtcgcat
    14641 tgccagcaag atcaatactc ttctcgccag acgcggtttg atcgctggaa gaggtcgagg
    14701 gggcttttag cgcagaaccg ataccgatga tcaacagcag gagaaccaga atgccgacgc
    14761 ccatcatcat atactgacga gaagcgggtt tactggctgc ttttttgcgc ttacgtggac
    14821 gacgctctac gcgctcttca tccacggttt catcttcgga ttcgtcaatt tcttcttcga
    14881 gttccggttc ctcattgcgc tctttttgcg cacgagtcgg acggcgatcg tcagtgtcat
    14941 caagttcaat atcatcaaaa ttgatctgcg gttcgccacg ttcagtacgc tcagaacgtt
    15001 cagaagattg acgagaacga ccagtacgac gatcgctggg atcgggtttc agctcgtctt
    15061 ctggtttgaa ttcatccatt taacacccca ctaaaaggtt aatgcttacc acgttgcaat
    15121 taacctgaag ctaatagacc gcttgataag cggcctgacc tttcttgttg ttacgctgat
    15181 tgacaatcgg caatggcgtt aagaacaagc tcgtgcgaaa cgccgctgcg aacttcactc
    15241 ttaccaattg ccaacggaag aattaagcgc atctctcccg caaggacttt cttgtcacgc
    15301 agcatatgcg gtaaatacgc ctgcgcagac atttcgcgcg gcccattgac cggtaaccca
    15361 gtccgcgtga gcagggttat aatacgctgc gtttcggcag aactaaactg cccgagacgt
    15421 tccgacgtcc gcgccgccat caccataccc gcagcgactg cttcaccgtg taaccaattg
    15481 ccatacccca tttcagcttc aatggcatga ccaaaggtat gtcccagatt cagtaaagca
    15541 cgtaacccgg tttcgcgctc gtcggcggcg acaacttctg ccttcagttc acaacaacgg
    15601 cgaatacagt acgccattgc cggaccgtcc agacgcaaca acgcatccag attctcttcc
    15661 agccagttga aaaacgcacc gtcaagaata atgccgtatt tgatgacttc tgcaagcccc
    15721 gacgctaact cacgcggggg aagcgttttc agacagtcga gatccaccac cactgaagca
    15781 ggctggtaga acgcgccaat catgttttta ccgaggggat ggttgaccgc agttttgccg
    15841 ccaacggagg aatcgacctg cgacagcaac gtcgtcggga cttgaatgaa acgtacaccg
    15901 cgctgataac tcgccgccgc aaagccggtc agatcgccca ctacgccgcc gccaagcgcc
    15961 accagcgtag tatcgcgacc atgcggcttt tgtaacaacg ccgtaaagac ggtatcgagc
    16021 acagccaggc ttttatactg ctcgccgtca gggaggataa cgctatcgac gttaacaccc
    16081 gcctgttcaa gtacgccgcg gaccttatcg agatacagag gagccagggt ttcgttggtg
    16141 accaacatga cctgctcgcc cgatttcagc ggtaagaatg aagctggttc attaaacaaa
    16201 ccagatgcga tggtaattgg gtaactacgt tccccgagag taacgacaat cctctccata
    16261 acgcgacatc caccttaatt actgtacccg cagacgagtg tatataaagc cagaattagt
    16321 tgctttccag catgtgaata atctggtttg caaccacttt agcgctttga tcatcagtac
    16381 gaatggtcac gtcggcaatc tcttcataca gcggattgcg ttcattggcc aacgcttcca
    16441 gaacttcacg cggcggtgtt tcaacgtgca gcaacgggcg ttttttatca cgctgcgtgc
    16501 gtgcaagttg cttttcgatg gtcgtttcaa gataaacgac aacgccacgt gcggaaagac
    16561 ggttacgcgt ttcgcgggat ttcacagagc cgccgccagt agccagcaca ataccctgtt
    16621 tctcggtcaa ctcattgatg accttttctt cgcgatcgcg gaagccttct tcgccttcta
    16681 aatcgaaaac ccagcccaca tcagctccgg ttcgtttctc aatctcttga tcggaatcgt
    16741 aaaattccat attgagttgt tgagctaact ggcgcccaat agtgcttttt ccggcaccca
    16801 taggcccaac cagaaagata ttgcgtttct ctgccatttt ttcggtacta ctaagactat
    16861 tcgttaatga taaacccgct tcgctcagag agcgccgcag gacatgaact gaaacctcat
    16921 aagatattgc gagagtcaga ctgaaaatta tctcaatact ccagcgggtt tggcaactga
    16981 ataaatcacc catccctctg catatctggt cgctgcaagc gcgctgcctt gctaccaccg
    17041 ctctggcgat aaatcaccgg gtaagattag cgtaaaaaag acagcaaaat gccgcctgaa
    17101 tgataaatca tcatcatgga acgcaggcag cgaagagcac caactctcaa atcggtactc
    17161 cttgtatgct aaatacctgc gcgcgtcaaa tagatgaaac acgttcagcg taaaaacatt
    17221 accgctttta cggctgttta ctcactggaa accagtcgtg gcgtgataaa caccactaac
    17281 tcgcgtcgtt catcttcttt tccgtcatga cgaaataatt gcccgaacca gggaatgtca
    17341 ccaagcaacg gtacgctatc ctgacccgat ttatttttac gggtaaaaat gccgcccagc
    17401 gccaacgttt ctccgctttt gacctcgacc tgcgtttcga tctcctgctt atcaatcgcc
    17461 aacacttcgc catcggcctg ctgtagcacc tgccccggaa cgttctggct gatgtgtaat
    17521 ttcagccgga tgcgaccttt ttgtaacacc gtgggcgtaa cttccatccc caggacggcc
    17581 tctttaaatt ccaccgacgt cgcgccactt tccccgctgg aaacctgata tggaatttcg
    17641 ctcccctgtt taatgctggc aggctgaaga tgtgaggcca gcagacgcgg actggcgata
    17701 atatccaact gctgtttttg ttcgagcgcg gaaagctcaa gatccagtaa acgtccgttg
    17761 atgcgtccaa tgttaaaacc gacatgcgtt gtcgccgtcg ctacggagag gtcgctacca
    17821 agcgcggtga cttgcccaac gccaccagcg tgttgcgcat cagccagcgt ccatttcaca
    17881 cctaactcac gcaaactttt ttcattaatg gtgacaatat gcgccgacag ctcaacctgc
    17941 ccgaccggca gatccatttg cgctacccac tgttcaagcg cgcttaacgc cgctttgtta
    18001 tctcgcagca aaaggcgatt ggtgcgttta tcgacggtca tactcccttt ggcactcagt
    18061 agcttctccc ccgctttcgc cagctctccg gcgtcggcgt attgcagagt aatattgcga
    18121 ttttccagcg gcagatttgc ctgcgcccgc gtctgctccg cctcctggcg ggcgatattg
    18181 tcattctgcc aggcaacgga atgcaccgag agaatgttgc cctcctggcg cgttatcaat
    18241 ccggcgcttt tcactacagt ttgtagtgcc tgcttccagg gaacgtcagt taaatgtaac
    18301 gacaccgtac cgctgacgtc tggcgaaacc accaggttca acttctcctg ttcagccagc
    18361 gcctgcaaca cctgagccac cggaacgtca tccaccatca gcgtcacttt ttgcggcttt
    18421 gccgcctgta cgccgggtat cagcatcaac agtagtgcgg ctatccattg cttcatttgt
    18481 atctccttgc cgttgccaca accattgtgg cggttcgcag tttgtcccgg ttcccagcgt
    18541 tagtgcgtct ggcgtcagct gtaaaattgt ccagccgttt tccagcacgt cgttttgctg
    18601 cacccgtcgc catttttttt gcccgtcttt tattacaccg atgatgcgct cacctcgccc
    18661 taccatcccc tgatagcgcc actggctaag ttcgctaatc cggcatagat cttccggcgg
    18721 tttaaaaggg tcacgcatac cggttaaaag gcacaatgta atacctgcca acaaccagcg
    18781 tttaaccctc atgcggcgtc tccagttgta gcgtgaacaa aaggtcatca ccttccacgc
    18841 ttaacgaaaa acggctcacg ctgacgttgc gctctgccag ccgtgtaaat gccgacggca
    18901 ctgcttccca cagcgttttc aacgccaact cacctccctg cgcggatgga tgccagtaaa
    18961 ccagttgcgc gccggataac tgaaaatcca gtggcgaaaa gggcagcgtt ttttcctcgc
    19021 tgaagggagt ggtgtcaacc aggcgataca gtgcggccca ctgctggtga tgacttgccc
    19081 gtagtcgaat taatgcgtcg ctctcttcat ggtgtgtcga tgacagaaaa atgagcgtaa
    19141 ctaacatcag caaccagact gcccagcaaa gctggcggag gcggggtgat gtggcgaacc
    19201 accagtcaaa gagcatgtcc atcgctaacc ttccttgtta actgatactc aaattgccag
    19261 cgtccctgcg catcctgctg cgtggccccc cgctgattga gatgaaaaga agcatcctgg
    19321 cgtagtgacg tttctagtgc gtttaacgcg gtaatgcttg ttgtcagccc cttgatctcc
    19381 agcgttccct gctgccagct tatcgttgtc agccaggcat gttcgggtaa aagcgccgcc
    19441 agtgcttcca gcgcagattg ccagtcgcgg gtaaattgtc gctggcgctg acgttgcaaa
    19501 cgctgttcgc gtaactgctg gcgttccagc aaacgtggtt tcgttatctg taagctgcgg
    19561 gcgagttgtt gttccgcctg aagcaaaacg gcgtttacgc gtgcttcggc attgcttgtc
    19621 agacgcagta ttagcgttat cccgacggcc agcagcagag gcgcaacgaa catcagcaac
    19681 cagaaacgaa gaaaagcagt ccggcgttgc tgtcgccagg gcaaaaaatt aattggcggg
    19741 ttcatcagta caccttccca agcgccagcc ccagcgcgat ggcaaagtct ccacctggtg
    19801 gcggtagcgg cggctgacga acagaaaccg cctcccaggg gtcaaatccg ccttcgccac
    19861 atagcgcgac gctttcggga tcaacagata acgctgccgc cagctccttc gcgctagtga
    19921 tccccaccgc cagtttgcgc ccccagctat agcgcgtcgc ccacagccac tgttcgttat
    19981 cacgccaggc cagacattgc tgatgagaag gtaaaaaagg caggaatcgc tgtaatgcac
    20041 tggcatccgg agtgatcgcg ctaacatgaa cacgcaacct ttctgccaga gtaagcagcg
    20101 ttgccagctc tttgctttgc gcggcagtca cgttataggc gggactgagt gagtcttcgc
    20161 tgtaatcgaa gcgcagggag tccggatcca tatccagctc acgggccatc gtcccggaca
    20221 gccaggccat ttgctcccgc tcaccgaggg acatcgacgg gcgtggaaat gaccgctgta
    20281 atgtgcgact ggcgggaaac gccaacataa tgtgatgacg ctgcggcagt tcacgactcc
    20341 acggtaacaa cgtgttagcc agccgctgca catcaacaat ccgcccatct ttgataatgt
    20401 cgttctccag tggcaaccgc caccagcggt gcaataagca ttcttttgcg ctccgcacga
    20461 tagcaaccgc taccgcttct tgctgttgta aatgcaaacc aatttgccag ttcttaaatg
    20521 ccattgtgat gatctcctta tcacccgtca ctctgacggg tatatcaatg cgtctggctt
    20581 gcctttatac taccgcgcgt ttgtttataa actgcccaaa tgaaactaaa tgggaaattt
    20641 ccagtgaagt tcgtaaagta ttttttgatc cttgcagtct gttgcattct gctgggagca
    20701 ggctcgattt atggcctata ccgctacatc gagccacaac tgccggatgt ggcgacatta
    20761 aaagatgttc gcctgcaaat tccgatgcag atttacagcg ccgatggcga gctgattgct
    20821 caatacggtg agaaacgtcg tattccggtt acgttggatc aaatcccgcc ggagatggtg
    20881 aaagccttta tcgcgacgga agacagccgc ttctacgagc atcacggcgt tgacccggtg
    20941 gggatcttcc gtgcggcaag cgtggcgctg ttctccggtc acgcgtcaca aggggcgagt
    21001 accattaccc agcagctggc gagaaacttc ttcctcagtc cagaacgcac gctgatgcgc
    21061 aagattaagg aagtcttcct cgcgattcgc attgaacagt tgctgacgaa agacgagatc
    21121 ctcgagctct atctgaacaa gatttacctc ggttaccgcg cctatggtgt cggtgctgcg
    21181 gcacaagtct atttcggtaa aacggtcgac caactgacgt tgaacgaaat ggcggtgata
    21241 gccgggctgc cgaaagcgcc ttccaccttc aacccgctct actcaatgga tcgtgccgtc
    21301 gcgcggcgta acgtcgtgct gtcgcggatg ctggatgaag ggtatatcac ccaacaacag
    21361 ttcgatcaga cacgcactga ggcgattaac gctaactatc acgcgccgga gattgctttc
    21421 tctgcgccat acctgagcga aatggtgcgc caggagatgt ataaccgtta tggcgaaagt
    21481 gcctatgaag acggttatcg catttacacc accatcaccc gaaaagtgca gcaggccgca
    21541 cagcaggcgg tacgtaataa cgtgctggac tacgacatgc gccacggtta tcgcggcccg
    21601 gcgaatgtgc tgtggaaagt gggcgaatcg gcgtgggata acaacaagat taccgatacg
    21661 ctgaaggcgc tgccgaccta tggtccgctg ctgcctgccg cagtcaccag cgccaatcct
    21721 caggaagcaa cggcgatgct ggcagacggg tcgaccgtcg tattgagtat ggaaggtgtt
    21781 cgctgggctc gtccttaccg ttcggatact cagcagggac cgacgccgcg taaagtgacc
    21841 gatgttctgc aaacgggtca gcaaatctgg gttcgtcaag ttgacgatgc atggtggctg
    21901 gcgcaagtgc cggaagtgaa ctcggcgctg gtgtcgatca atccgcaaaa cggtgccgtt
    21961 atggcgctgg tcggtggctt tgatttcaat cagagcaagt ttaaccgcgc cacccaggca
    22021 ctgcgtcagg tgggttccaa catcaaaccg ttcctctaca ccgcggcgat ggataaaggt
    22081 ctaacgctgg caagtatgtt gaacgatgtg ccaatttccc gctgggatgc aggtgccggt
    22141 tctgactggc agccgaagaa ctcaccaccg cagtatgccg gtccaattcg cttacgtcag
    22201 gggctgggtc agtcgaaaaa cgtggtgatg gtacgcgcaa tgcgggcgat gggcgtcgac
    22261 tacgctgcag aatatctgca acgcttcggc ttcccggcac aaaacattgt ccacaccgaa
    22321 tcgctggcgc tgggttcagc ttccttcacc ccaatgcagg tggcgcgcgg ctacgcggtc
    22381 atggcgaacg gcggcttcct ggtggacccg tggtttatca gcaaaattga aaacgatcag
    22441 ggcggcgtaa ttttcgaagc gaaaccgaaa gtagcctgcc cggaatgcga tattccggtg
    22501 atttacggtg atacgcagaa atcgaacgtg ctggaaaata acgatgttga agatgtcgct
    22561 atctcccgcg agcagcagaa tgtttctgta ccaatgccgc agctggagca ggcaaatcag
    22621 gcgttagtgg cgaagaccgg cgcgcaggag tacgcaccgc acgtcatcaa cactccgctg
    22681 gcattcctga ttaagagtgc cttgaacacc aatatttttg gtgaaccagg ctggcagggt
    22741 actggctggc gagcaggtcg tgatttacaa cggcatgata taggcggaaa aacgggtacc
    22801 actaacagtt cgaaagatgc gtggttctcg ggttacggtc cgggcgttgt gacctcggtc
    22861 tggattggct ttgatgatca ccgtcgtaat ctcggtcata caacggcttc cggagcgatt
    22921 aaagatcaga tctcaggtta cgaaggcggt gccaagagtg cccagcctgc atgggacgct
    22981 tatatgaaag ccgttcttga aggtgtgccg gagcagccgc tgacgccgcc accgggtatt
    23041 gtgacggtga atatcgatcg cagcaccggg caattagcta atggtggcaa cagccgcgaa
    23101 gagtatttca tcgaaggtac gcagccgaca caacaggcgg tgcacgaggt gggaacgacc
    23161 attatcgata atggcgaggc acaggaattg ttctgattaa aaaggcgctt cggcgccttt
    23221 ttcagtttgc tgacaaagtg cacttgttta tgccggatgc gggtgaacgc gttatccggc
    23281 caacaaaaat tgaaaattca ataaattgca ggaacttcgt aagcctgata agcattatgc
    23341 atcaggcaaa cttcacgcat ttacactcgc ccctgccctt tcaaccattc gcgcacgagg
    23401 aacagcgcgc tgacattacg cgcttcattg aagtcagggg cttccagcaa atccatcata
    23461 tgcgccagcg gccagcgcac ctgtggtagc ggctctggct catcgccttc cagtgattcc
    23521 gggtagagat cttgcgctac cacgatattc attttgctgg aaaagtaaga cggtgccatg
    23581 ctgagcttct tcaaaaaagt cagatcgttc gcaccaaatc caacctcttc ttttagctcg
    23641 cggttagcgg cttcaaagac gctttcaccc ggatcaatta atcctttcga aaaacctaat
    23701 tcgtaggatt cagttcccac tgcgtattcg cggatcagga tcaggtgatc gtccacaatc
    23761 ggcacaatca tcactgcttc ccggttggtt ggacgcattc gttcataaac acgccgcacg
    23821 ccattgctga actccagatc cacgctttcg acggtaaaca gtcgggaacg ggctacagtt
    23881 tcaacattca gaatggtggg tttttgtaat gatttgctca tcgtgggatc tatgctgtga
    23941 aatcagcggt tattgtgcga tataggacac gctttcggca atgtgaattg catgttattt
    24001 acatttatgt aacttaataa ataatcgtcc tcaaatcaaa ttaaaagtca ataggttgaa
    24061 ataactccag gaatttgctg atattccgcc ttcagagggt ttgctatgat cagcggttac
    24121 tgtgatgtgc ttaatgatgc tcaagttaaa ctccacgctt gccgatagcc aaccgcagaa
    24181 tcatgttttg tgtccggtgc gactgaccac gcctgacaga ctaagtaaga tggggaaagc
    24241 atgagcacca ttgtgatttt tttagctgct ttgctggcct gctcactact tgcgggatgg
    24301 ctgataaaag tgcgatccag acggcgtcag ctgccctgga ccaacgcctt cgcggatgcg
    24361 caaacgcgta aactcacacc tgaagaacgt agcgccgttg aaaattatct tgagagcctg
    24421 acgcaggtat tacaggtgcc tggcccaacg ggagccagcg cggcaccgat ctctctggcg
    24481 ctgaatgccg aaagcaacaa cgtcatgatg ctaacacacg ctatcacgcg ttacggcatc
    24541 tctaccgacg atccgaataa atggcgttac tacctcgatt cggtagaagt ccacctgccc
    24601 cctttctggg agcagtacat caacgatgag aataccgttg aactgattca taccgattcg
    24661 ctgccgctgg ttatttcact caacggtcat acgctgcagg agtacatgca ggaaactcgc
    24721 ggctatgcct tgcaacctgt tccgtcaaca caggcgtcga ttcgcgggga agagagtgag
    24781 caaatcgagc tactcaatat tcgcaaagaa acgcacgaag agtatgcgct aagtcgtccg
    24841 cgcgggctgc gcgaagcgtt gctgattgtc gcctccttcc tgatgttctt tttctgcctg
    24901 attaccccgg atgtatttgt tccgtggctg gcaggcggcg cgttactgct gctgggcgca
    24961 ggtctgtggg ggctattcgc gcccccggca aaatcctccc tgcgggaaat tcattgtctg
    25021 cgcggtacac cccgtcgttg gggattgttt ggcgaaaacg atcaggaaca gatcaacaat
    25081 atttcactcg gtattatcga cctggtctac cccgcacact ggcagccata cattgctcag
    25141 gatctcggtc aacaaaccga tatcgatatc tatctcgacc gccacgtagt gcgtcagggg
    25201 cgatatcttt cgctgcatga tgaagtaaaa aactttccgt tacagcactg gctgcgcagt
    25261 acgattatcg ctgcgggctc gctacttgtg ctgtttatgc tgttattctg gatcccgctg
    25321 gatatgccgc tgaaattcac cctctcgtgg atgaaaggcg cgcagaccat tgaagccacc
    25381 agcgtgaaac aactggctga cgctggcgtg cgggttggcg atacattgcg tattagcggt
    25441 acgggaatgt gtaatattcg cacttccggt acctggagcg cgaaaaccaa ctcacctttt
    25501 ttaccgtttg actgctcgca gatcatctgg aatgacgccc gctcattgcc attaccagaa
    25561 tctgaattgg tcaacaaagc gacggcattg accgaagcag ttaatcgcca gctgcaccct
    25621 aaaccggaag atgaatctcg cgtcagcgcc tcattacgtt cagcaattca aaaatccggc
    25681 atggtattgc ttgatgattt tggcgacata gtactgaaga cagcggatct gtgttctgcc
    25741 aaagatgact gtgtgcgact gaaaaatgcg ctggtcaatc tcggcaacag taaagactgg
    25801 gacgcgctgg taaaacgcgc caacgccggg aagctcgatg gcgtgaatgt gttattacgc
    25861 ccggtgagtg cggaatcgct ggataacctg gtggcaacct ccaccgcgcc gtttatcacg
    25921 catgaaacag cgcgagcggc acaatcacta aacagtccgg cccccggcgg attcctgatt
    25981 gtcagcgacg aaggcagcga ttttgttgat cagccctggc cttcggcatc actttacgac
    26041 tacccgccgc aagaacagtg gaacgctttc cagaaactgg cacaaatgct gatgcatacg
    26101 ccgtttaacg ccgagggtat cgtcacaaaa atcttcactg acgccaatgg tacgcagcat
    26161 ataggccttc atccgatccc ggatcgttcc ggcctgtggc gctatctcag caccacgctg
    26221 ctgctactga cgatgctggg tagcgccatt tataatggcg tacaggcctg gcggcgttac
    26281 cagcgtcatc gtactcgcat gatgaagatt caggcctatt atgaaagctg cctgaaccca
    26341 caactgatca ccccttcaga aagccttatc gaataacacg tttgcgcggc aggttatgct
    26401 accctgtcgc gcaaattgct tcactctgga gatttccctc atgcatatca acattgcctg
    26461 gcaggacgta gataccgttc tgctggatat ggacggcacg ttgctcgacc tcgccttcga
    26521 taactatttc tggcaaaagc tggtgcctga aacatggggc gcgaaaaacg gggttacgcc
    26581 acaggaagcg atggaatata tgcgccagca atatcacgac gtacagcata cgctaaactg
    26641 gtactgtctt gattactgga gtgagcaact gggtctggat atctgtgcga tgaccaccga
    26701 gatgggaccg cgcgccgtac tgcgtgaaga taccattccg tttcttgagg cactgaaagc
    26761 cagcggtaag cagcgaattt tgctcaccaa tgcgcatccg cacaacctgg cggtaaaact
    26821 tgagcatacc ggtctggacg cacaccttga tttattactt tccacccaca catttggtta
    26881 tccgaaagag gatcagcggt tatggcatgc ggtggccgaa gctacgggtc tgaaagctga
    26941 aagaacgctg tttattgatg acagcgaagc gattctcgat gctgccgcgc aatttggtat
    27001 tcgttactgc ctcggcgtga ctaatcctga ctccgggatt gccgagaaac agtatcaacg
    27061 ccatccgtca ctgaatgact accgccgcct gatcccctcg ctaatgtgaa ggagacgcca
    27121 tgaaagaaaa acctgctgtt gaggttcgac tggataaatg gctatgggct gcccgttttt
    27181 ataaaacccg cgcgctggcc cgtgaaatga ttgaaggcgg taaggtgcat tacaacgggc
    27241 agcgcagcaa gccgagcaaa atcgtcgagc tgaatgccac gctcactctg cgccagggaa
    27301 atgacgaacg cacggtgatt gtaaaggcga ttactgaaca gcgtcgcccc gccagcgagg
    27361 cagccttgct gtatgaagag actgcggaaa gtgtagagaa acgcgaaaaa atggcgctgg
    27421 cacgtaaact taatgcctta accatgccgc acccggaccg acgcccggac aaaaaagagc
    27481 gccgcgacct gttacgattt aaacacggcg acagtgaata actgtcacct gcaagagaga
    27541 tgattatgcc gcaacatgac caattacatc gctatctgtt tgaaaacttt gccgttcgcg
    27601 gcgaactggt aaccgtttcg gaaaccctgc aacagatcct tgagaaccac gattatccgc
    27661 agcccgttaa aaacgtgctg gcagaactgc tggttgcgac cagcctgtta accgctacgc
    27721 tgaagtttga tggtgatatc accgtacagc tgcaaggcga cggtccgatg aatctggcgg
    27781 ttattaacgg taacaacaaa cagcagatgc gcggtgtggc gcgcgtgcag ggcgaaattc
    27841 cagaaaatgc cgacctgaaa acgctggtcg gcaatggtta cgtggtgatc accatcaccc
    27901 cgagcgaagg tgaacgctat cagggcgtgg tgggtctgga aggtgatact ctggcggcct
    27961 gcctggaaga ttactttatg cgttctgaac agctgccgac gcgcctgttt attcgcaccg
    28021 gcgacgtaga cggcaaaccg gctgcaggcg gtatgttgtt gcaggtaatg cctgcgcaaa
    28081 atgcccagca ggacgacttt gaccacctgg cgacgctaac cgaaaccatc aaaaccgaag
    28141 aactgttgac cttaccggca aacgaagtgt tgtggcgttt gtatcacgaa gaagaggtga
    28201 cggtttatga tccgcaggat gtggagttca aatgcacctg ctcgcgtgaa cgttgcgccg
    28261 acgcgctaaa aacgctgccg gatgaagaag tcgatagcat cctggcggaa gatggcgaaa
    28321 ttgacatgca ttgtgattac tgcggtaacc actatctgtt caatgcgatg gatattgctg
    28381 aaatccgcaa caacgcgtct ccggcagatc cgcaagttca ttaatgagta tgtccggcag
    28441 agagggctct gccggatttt acgacgtaat ggcctgatgc gctgcgctta ccaggcctat
    28501 gaaataaatc gcaagttatt ttggtcagat acagcgtcgt atccgttaaa agcgttgtca
    28561 cttacttaag tttatttcca gcgtttcacc atctaccgtc agatcgcgcc acgctttcgg
    28621 cctcgtcccc agttttccat ccttcgcagc tgtgagtaat ttttctttcg ttattttgtg
    28681 cggaagtaaa ctcatatcaa gcgcgtccca ttcttttctg gctgagtcaa agccatagac
    28741 aatgactctg tcatcattaa aagcaaacag gatccgctcc gcctgaccat cactattcag
    28801 atcttgctcg accagtacgc aggcgtcttt ttcaatacag gtcatcacgt tatagcgatc
    28861 ctggattaag gccgaccaga atgtcgcatc cggtttacca gaacctgggg caattaacac
    28921 attatcggct aatacttttt ccgatacctg ttcctgaaga tgttgctctc catctaacgc
    28981 catcagcaga tcccgcgcgc gtttcgggtc tttcatatac tcggcatcgc ttttcagcga
    29041 ctcaagcgcc gcacgtccat agcgaccgct ctgttcgagc atatagagac ttacctggtc
    29101 tggcgtgttt ttgccgctct gataacgcgc catatgactg ttcacgctaa tgcgcatact
    29161 gtccagcacc ggagaattaa gaagcaccag tattaccaac accaataacg aaaccgcaag
    29221 gttcactttg ccctgaagaa caagggggtt ttgcccttta cgccagacga tactgacaaa
    29281 ataccccagc gaccatacca gtaacaccag caccgccagc gcgccctgca agcggtcgac
    29341 agtccagccg tactgagcga cccgcagcca taacgcccag gcggcgacga acacatacag
    29401 cggagcaacc agcaaagcgg ttttaatcag gcaacgcaac ggccccgtcc agggaagaga
    29461 cgctttttgc ggatcgcgga caatagccat taagatcaat tgcaaaaagg ccagcgttaa
    29521 cagcaaccca gcggcggaga tgtggcgaga aatcgcgctc aggcccgtaa acggcagggt
    29581 gataataaac atcagggtta gcaatgatac taacggcagc aaccccgtgg cgattaacgt
    29641 gaacaacttt tgaatagagt cgattaaacg tgactgtgtt cgcgcgagga tcaccgccag
    29701 cgcggtaacc agacctaacg tgagataaat aaaccagtcg gttgcaaaaa agagcgtttt
    29761 gaaaaacgtg atgccaacaa gtttaaacaa ctcactccac agtaaaagca ccagccacgt
    29821 caggccattg gcgagaaaaa tcaccaggaa tataagtacg ttatgccata ctgactggta
    29881 aaaatagcga tagcggctac tgtcattgcg gatgcgaagg ctttgttgta tccacggtag
    29941 caacaacatt gccatcagca gcagatagaa gccaaaatcc caaagcgcct tttcagctct
    30001 ccaggggttc atgccatcaa tttgccactt tagccaaccg ctcatcccta gcgtggcaat
    30061 aaacaccagc gccagccatc cccaaaagcg cttctgttta aaggagatca cggagaagag
    30121 taaaacggat gaaaacgcga cggtcgcagg cacgccataa acaatccaac tgagattttt
    30181 tccggcaagc caggcgatca gcaggtagca aaccagcccc tgaagtaatc cggtagcaat
    30241 catcccccag cgtgttgccg gtgaaagttc aacgttatcc atgttggtta tccagaatca
    30301 aaaggtgggt taattatcgc atccgggcag tagtattttg cttttttcag aaaataatca
    30361 aaaaaagtta gcgtggtgaa tcgatacttt accgattgaa tttgcatcaa tttcattcat
    30421 gaatgcgatt ccactcacaa tattcccgcc atataaacca agatttaacc ttttgagaac
    30481 attttccaca cctaaaatgc tatttctgcg ataatagcaa ccgtttcgtg acaggaatca
    30541 cggagttttt tgtcaaatat gaatttctcc agatacgtaa atctatgagc cttgtcgcgg
    30601 ttaacacccc caaaaagact ttactattca ggcaatacat attggctaag gagcagtgaa
    30661 atgcgcgtta acaatggttt gaccccgcaa gaactcgagg cttatggtat cagtgacgta
    30721 catgatatcg tttacaaccc aagctacgac ctgctgtatc aggaagagct cgatccgagc
    30781 ctgacaggtt atgagcgcgg ggtgttaact aatctgggtg ccgttgccgt cgataccggg
    30841 atcttcaccg gtcgttcacc aaaagataag tatatcgtcc gtgacgatac cactcgcgat
    30901 actttctggt gggcagacaa aggcaaaggt aagaacgata acaaacctct ctctccggaa
    30961 acctggcagc atctgaaagg cctggtgacc aaacagcttt ccggcaaacg tctgttcgtt
    31021 gtcgacgcct tctgtggtgc gaacccggat actcgtcttt ccgtccgttt catcaccgaa
    31081 gtggcctggc aggcgcattt tgtcaaaaac atgtttattc gcccgagcga tgaagagctg
    31141 gcaggtttca aaccagactt tatcgttatg aacggcgcga agtgcactaa cccgcagtgg
    31201 aaagaacagg gtctgaactc cgaaaacttc gtggcgttta acctgaccga gcgcatgcag
    31261 ctgattggcg gcacctggta cggcggcgaa atgaagaaag ggatgttctc aatgatgaac
    31321 tacctgctgc cgctgaaagg tatcgcgtct atgcactgct ccgccaacgt tggtgagaaa
    31381 ggtgatgttg cggtgttctt cggcctttcc ggcaccggta aaaccaccct ttccaccgac
    31441 ccgaaacgtc gcctgattgg cgatgacgaa cacggctggg acgatgacgg cgtgtttaac
    31501 ttcgaaggcg gctgctacgc aaaaactatc aagctgtcga aagaagcgga acctgaaatc
    31561 tacaacgcta tccgtcgcga tgcgctgctg gaaaacgtca ccgtgcgtga agatggcact
    31621 atcgactttg atgatggttc aaaaaccgag aacacccgcg tttcttatcc gatctatcac
    31681 atcgataaca tcgttaagcc ggtttccaaa gcgggccacg caacgaaagt tatcttcctg
    31741 accgcagacg cctttggtgt gctgccaccg gtttctcgtc tgactgccga tcaaacccag
    31801 taccacttcc tctctggctt caccgccaaa ctggccggta cagagcgcgg catcaccgaa
    31861 ccgacgccaa ctttctccgc ctgcttcggc gcggcattcc tgtcgctgca cccaactcag
    31921 tacgcagaag tgctggtgaa acgtatgcag gcggcaggcg cgcaggcgta tctggttaac
    31981 accggctgga acggcactgg caaacgtatc tcgattaaag atacccgcgc cattatcgac
    32041 gccatcctca acggttcgct ggataacgca gaaactttca ctctgccgat gtttaacctg
    32101 gcgatcccaa ccgaactgcc gggcgtagac acgaagattc tcgatccgcg taacacctac
    32161 gcctctccgg aacagtggca ggaaaaagct gaaacgctgg cgaaactgtt tatcgacaac
    32221 ttcgataaat acaccgacac ccctgcgggt gccgcgctgg tagcggctgg tccgaagctg
    32281 taatcagctg aaatctcatt gaccccttat ccaaacggat aaggggtttt tttatatcta
    32341 attagctacc aatatgtaca cggtacactt ataattatca atagaccgca ggatgcatta
    32401 tgttcacgtt tattgaacta caaggattta gcaaacgccg ccagttattg ctacctgatg
    32461 atgagttccg ggcttttcag gaactactca ttcaagatcc cactgccggt gacattatcg
    32521 caggcacagg cagttttaga aaaattcgct ggaaccgttc tggaatgggg aaacggggtg
    32581 gcattcggat aatttattac aacgtaacac gcaatggccg aatttatctg gcgttgattt
    32641 atcctaaaaa tgaacaggat gatttaacag aagaacaaag aaaggcattg aaactattgt
    32701 ccgagaagct gctttaatgt tgaagctcca caaattccac catggccgaa atgtcaacgg
    32761 aggcattgaa aacagatgaa agatgaatta tttgctgatc ttcttgccag cgcagaagaa
    32821 atggtacgca tcgaaaaggg tgaagaaacg cctgaaccag agcacgtcca tacttttagc
    32881 gaaatcgatg taaaagcgat ccgtgaagca acgggtttac gtcagcaaga ttttgctgtc
    32941 gccgtagggg taagttatga cctggtcaaa agctgggaaa ccaaacgccg tcaacctacc
    33001 ggagcacccc gaaaattgct cctgcttttg caagcgaatc ctttcattat caatcaattg
    33061 aaaacaatct aaaaacagga ggcaccttcg cctcccgttt tatttaccct tcttttgtcg
    33121 caccctgcgc ccgcgttacc ggcacaggca gccaggcgcg aatggaaagc ccgccccgct
    33181 cgctggtgcc aagctccagc atgccgttat ggttatccac gatacgctgc acaattgcca
    33241 gccctaatcc cgtgccgcta atggtgcgcg cactgtcgcc gcgaacaaac ggctggaaca
    33301 ggtgcttacg ttgttccggc gcaattcccg gaccgtcatc ttctacctgg aaccaggcgc
    33361 gattcggctc cgttccgctg ctgactttga tccagccatt gccgtaacgg gcggcgttga
    33421 ccaccatatt cgccaccgcg cgtttgatcg acagcgggtg cattttcacc tcaatgctgc
    33481 cggggtaaag cgcggtttca atttcccgct catagccact ttcggcggca atcacctcac
    33541 cgagcactgc gttgagatcc gccatttcca tcggcatctc ctgcccggtg cgcaggtagt
    33601 cgataaactg ctcaatgatg gcgttgcact cttcgatatc tttattgatc gattctgcca
    33661 ggtagccatc ctgctcgctc atcatctcgg ttgccaggcg aatgcgcgtc agcggcgtac
    33721 gcagatcatg acttaccccc gccatcagca gcgtgcgatc atccgccagt tgcttaacac
    33781 cagccgccat atggttaaag gcgcgggtaa ccgaacgcac ctccgaagcg ccatattcac
    33841 gcagtggcgg cggaataatc cctttaccga cctgcagagc agcatgttcg agatcgacca
    33901 acggtcggtt ctggatacgg ataaacagcc acgccccgcc tatcgccaat agcataatcg
    33961 ccagcgtata gcggaacagc ggagaaaaat caccctgatg aatttcggtc agcggcacac
    34021 gcacccagat attgggcgac agccaggttt tcagccagac gacaggcgaa cttttgttga
    34081 cctcaacgcg tacttccgtc gggccgccca gttgctgcgc catctgatgg cttaagaatt
    34141 catagtgttg tgcccaacgc agaccagcct cttcggcagc ttcgttggag tagagagaga
    34201 tccccagctc acggtagatc tcccgacgga aagcgggagg cacaaccaac tgcgtgccgt
    34261 cctccagttg cagtttgtcg gtcatcaaca tacgcacttc gtacgcgagg actttattaa
    34321 actgctggag gctcggcaaa atcgcgaagt tcagcaccac cagataagtc gtcaccaggc
    34381 tgacgaacag caaggtgacg atgagcaata acgtgcgggc aaatgaactt cgtggcgaga
    34441 agcgcaatcg cctcatgctt tagaaccgtc cggtacaaag acgtagccca gaccccagac
    34501 ggtctgaatg taacgcggat gcgccggatc ttcttctacc atgcggcgca gacgcgaaat
    34561 ctgcacgtcg atggaacgtt ccattgcgga gtattcacgg ccacgggcaa ggttcatcag
    34621 cttatcgcgg gagagcggct cacgcggatg gctgaccagt gccttcagta ccgcaaactc
    34681 accgctggtg agcggcatcg gctcgtcttc gcggaacatt tcgcgcgtac cgaggttaag
    34741 tttgaactta ccgaaagcaa ttaccgcctc ttcctgtgac ggcgcgcccg gcagttcgtt
    34801 cgcctgacga cgcagcaccg cacgaatgcg ggccagcagt tcacgcgggt taaacggttt
    34861 cggaatgtag tcgtcagcgc caatctccag accaacgata cggtccactt cttctccttt
    34921 cgccgtcacc ataatgatcg gcatcgggtt gctctggcta cgaagacgtc ggcaaatcga
    34981 caagccatct tcaccaggta acatcaaatc cagtaccata agatggaaag attcacgagt
    35041 cagcaggcga tccatctgtt ctgcattagc gacgcttcga acctggaagc cttgttcggt
    35101 gagataacgt tccagcagcg cgcgcaggcg catatcgtca tcgaccacca gaatcttgta
    35161 gttctcttgc attgtttgta ctcccaaagg ttcgcaacaa tttgtaagcg tgtattctta
    35221 aaaaagctca cgttcgtcac cagctaaatc tggtatgaat ttcagcctaa attgttacaa
    35281 agcatattaa acagcagctt aagtatacaa tttattcggc gaaacattat tgattctgtt
    35341 gatatgatca cgttataccc aatgtgcgca ttatcaaaca gacaaaggga atcaacgaga
    35401 tgaaaacgcc cctggttacc cgggaagggt atgaaaaact caaacaagag cttaattatc
    35461 tctggcgtga agaacgcccg gaggtcacaa aaaaggtgac ctgggccgca agtctgggcg
    35521 accgcagcga aaatgctgac tatcagtata ataaaaagcg cctgcgtgaa atcgaccgtc
    35581 gcgtgcgcta tctcactaaa tgcatggaaa atctcaaaat cgtcgattac tcccctcagc
    35641 aggaaggcaa agtctttttt ggtgcgtggg tggagattga aaacgacgat ggcgtgactc
    35701 accgtttccg tattgtcggc tacgatgaaa tttttggccg taaagattac atctctatcg
    35761 attccccgat ggctcgcgca ttgttgaaaa aagaggtggg cgatttggcg gtggtgaata
    35821 cccctgccgg ggaagcgagc tggtatgtga atgctatcga gtacgtgaaa ccgtaaggaa
    35881 gagtcttaac ctcctgccga tggctggcat ttttgccagc cagtccgtat aactatcccc
    35941 tgatttttga tccgaaaaga tgaactcaaa ccatgatgaa tgattcgttc tgccgcatta
    36001 ttgcgggtga aattcaggcg cgcccggaac aggttgacgc tgccgttcgc ctgcttgacg
    36061 aagggaatac cgtgccgttt atcgcacgtt atcgtaagga aatcaccggc ggtctggatg
    36121 acacgcagct gcgtaatctg gaaacgcgcc tgagctatct gcgcgagctg gaagagagac
    36181 gtcaggcgat cctcaagtcc atttccgagc aaggcaaact cactgacgag ctggcgaatg
    36241 ccatcaacgc cacactaagc aaaaccgaac tcgaagacct ctacctgccc tacaaaccta
    36301 aacgccgcac ccgcgggcaa atcgccattg aagcagggct tgagccgttg gctgacctgc
    36361 tgtggagcga tccgtcacac acgccagaag tcgccgctgc acaatatgtt gatgccgata
    36421 aaggcgtggc agataccaaa gccgcgctgg acggcgcgcg ctatatccta atggaacggt
    36481 ttgccgaaga tgccgcgctg ctggcgaaag tgcgtgatta tctgtggaag aacgcgcatt
    36541 tggtctcaac cgtggtgaat ggtaaagaag aggaaggggc gaaattccgc gactatttcg
    36601 atcatcacga accgctgtcc acggtgcctt ctcaccgcgc gctggcgatg ttccgtggtc
    36661 gtaacgaagg cgtacttcag ctttcgctga atgccgatcc gcagttcgaa gagccgccga
    36721 aagagagcta ttgcgagcaa atcatcatgg atcaccttgg cctgcgcctg aacaatgccc
    36781 cggcggatag ctggcgcaaa ggtgtggtga gctggacgtg gcgcatcaag gtgttgatgc
    36841 atctggaaac tgaactgatg ggcaccgtgc gcgaacgcgc ggaagatgaa gcaatcaacg
    36901 tctttgcccg taacctgcac gatctgctga tggcggcccc tgccggactg cgtgcaacga
    36961 tgggcctcga tccgggtctg cgtaccgggg taaaagtggc ggtagtcgat gcgacaggta
    37021 aactggtggc gaccgatacc atttatccgc ataccgggca ggccgcaaaa gcagcgatga
    37081 ccgttgctgc gctgtgtgaa aagcataacg ttgaactggt ggcgatcggc aacggtacag
    37141 cttctcgcga aaccgaacgt ttctatctcg acgtgcagaa gcagttcccg aaagtgaccg
    37201 cgcagaaagt gatcgtcagc gaagctggcg cgtcggttta ctcggcttcc gagctggcag
    37261 cacaggaatt cccggatctc gacgtttcgc tgcgtggcgc ggtgtctatc gcccgtcgtt
    37321 tgcaggatcc gctggcggag ctggtgaaaa tcgatccgaa atctatcggc gtaggtcagt
    37381 atcagcatga cgtcagccag acgcagctgg cacgcaaact ggatgcagta gtagaagact
    37441 gcgtaaacgc cgttggcgtc gatctcaaca ccgcttctgt tcctctgtta acccgcgtgg
    37501 cgggcctgac gcgcatgatg gcgcaaaaca tcgtggcctg gcgcgatgag aacggccagt
    37561 tccagaaccg tcagcaactg ttaaaagtga gccgtctggg gccgaaagcc ttcgagcagt
    37621 gcgcgggctt cttgcgcatt aaccacggtg ataacccgct ggacgcttct accgttcacc
    37681 cggaagccta tccggtggtg gaacgcattc tggcagcaac acagcaggca ctgaaagatc
    37741 tgatgggtaa cagcagcgaa ctgcgtaacc tgaaagcgtc tgactttact gatgagaaat
    37801 tcggtgtgcc gacggtaacc gacatcatca aagagctgga aaaaccgggt cgcgatccgc
    37861 gtccggaatt taaaaccgct cagtttgccg atggcgtcga gacaatgaac gacctgcaac
    37921 cgggtatgat cctcgaaggt gcggtgacca acgtcaccaa ctttggcgcg tttgtcgata
    37981 ttggcgtgca tcaggacggc ctggttcaca tctcttcatt gtcgaacaag tttgtggaag
    38041 atccgcatac cgtggtgaaa gcgggcgaca ttgtgaaggt gaaagtgctg gaagtggatc
    38101 ttcagcgtaa acgtatcgcc ctgaccatgc gtctggacga gcagcctggc gaaaccaacg
    38161 cccgtcgcgg tggcggtaac gatcgcccgc aaaacaaccg cccggcagcc aaaccgcgtg
    38221 ggcgtgaagc gcagcctgcc ggtaatagcg cgatgatgga tgcgctggcg gcggcaatgg
    38281 gcaaaaaacg ttaaacgccc ggactggccc ctaaacctgc gcgattaggc gttcacgcca
    38341 catctggcaa cgaacgcctt gcctgacata aaagtgccgg agaatatctc cggccttttt
    38401 attccacaag caaactcata atatatcccg ttaatattta tcacttcatt aacaactgaa
    38461 accttaatta aacattaacc agccataata attcgtcatg cgaattatat tttcgctgcg
    38521 atataacctt gagccatatc aacattgagt cagattatta ttcaaaccaa cattcacaca
    38581 cattttaagt attgctgata gaaaccattc tcattatcat tgtactgttg attatttaat
    38641 ctctccttcg ttggcaaatc atctggtctc atgtcgctgt caaacgcccc atgaggtagt
    38701 tatccagtta atgagaaaca agtaggcacc tatgcaatac actccagata ctgcgtggaa
    38761 aattactggc ttttcccgtg aaatcagccc ggcatatcgc caaaaactgc tttctcttgg
    38821 catgttacct ggctcctctt ttaatgtggt gcgcgtcgct ccactcggcg accccattca
    38881 tatcgaaacc cgtcgtgtga gcctggtatt acgcaaaaaa gatctggcct tattagaagt
    38941 ggaagcggtt tcctgttaat acggtgataa caacaatgaa aaaattaacc attggcttaa
    39001 ttggtaatcc aaattctggc aagacaacgt tatttaacca gctcactggc gcacgtcagc
    39061 gtgtaggtaa ctgggctggc gttaccgtcg aacgtaaaga agggcaattc tccaccaccg
    39121 atcatcaggt cacgctggtg gacctgcccg gcacctattc tctgaccacc atctcatcgc
    39181 agacctcgct cgatgagcaa atcgcctgtc actacatttt gagtggcgac gcagacctgc
    39241 tgattaacgt ggtggatgcg tctaaccttg agcgtaacct gtacctgacg ctgcaactgt
    39301 tggaactcgg cattccctgc atcgtggcac tgaacatgct cgacattgcc gagaagcaaa
    39361 atattcgtat tgaaattgat gctctgtcgg cgcgtcttgg ctgcccggtg atcccgctgg
    39421 tttcaacccg tggtcgcggt attgaagcgc tcaagctggc gattgatcgc tataaagcta
    39481 acgagaatgt ggaactggtg cattacgcac agccgctact caacgaagca gattcactgg
    39541 caaaagtgat gccttccgac atcccgctga aacaacgtcg ctggctgggc ctgcaaatgc
    39601 tggaaggcga tatctacagc cgcgcctacg ccggtgaagc gtcgcagcat ctggatgccg
    39661 ccctcgcccg tctgcgtaat gagatggacg atccggcgct gcacattgcc gatgcgcgtt
    39721 accagtgcat tgctgccatc tgtgatgtgg taagcaacac cctgacggca gaacccagcc
    39781 gtttcaccac tgcggtagat aaaatcgtgc tcaaccgttt cctcggtctg ccgattttcc
    39841 tctttgtgat gtacctgatg ttcctgctgg ctatcaacat cggcggggcg ttacagccgc
    39901 tgtttgacgt cggctccgtg gcgctatttg tgcatggtat tcaatggatt ggctacacgc
    39961 tccacttccc ggactggctg actatcttcc tcgcccaggg cctgggtggc ggtattaaca
    40021 ccgtgctgcc actggtgccg cagatcggca tgatgtacct gttcctctcc ttccttgagg
    40081 actccggcta tatggcgcgt gcggcgtttg tgatggaccg cctgatgcag gcgctgggct
    40141 tgccggggaa atcctttgta ccgctgatcg tcggcttcgg ttgtaacgta ccatcggtaa
    40201 tgggcgcacg tacgcttgat gcaccgcgtg aacgcctgat gaccatcatg atggcaccgt
    40261 ttatgtcctg cggcgcacgt ctggctatct tcgcagtatt cgcggctgcc ttcttcgggc
    40321 agaacggtgc gctggcggtc ttctcgctgt atatgctggg tatcgtaatg gcggtgctga
    40381 cgggcctgat gctcaagtac accatcatgc gcggtgaagc gacgccgttt gtcatggagc
    40441 tgccggtcta ccatgtacca cacgttaaaa gcctgattat ccagacctgg cagcgtctga
    40501 aaggcttcgt tctgcgtgct ggtaaagtga ttatcatcgt cagcattttc ctgagtgctt
    40561 tcaacagctt ctctctgagc gggaaaatcg tcgataacat caacgactcg gcgctggctt
    40621 ccgtcagccg ggtgatcacc ccagtcttca agccgattgg cgtgcatgaa gataactggc
    40681 aggcaacggt tggcctgttt acaggtgcca tggcgaaaga agtggtagtg ggtacgctca
    40741 acaccctcta caccgcagaa aatattcagg acgaagagtt caatccggcg gaatttaacc
    40801 tcggtgaaga gctgttcagt gcgatagatg aaacctggca gagcctgaaa gacaccttca
    40861 gccttagcgt actgatgaac cccattgaag ccagcaaagg cgacggcgaa atggggaccg
    40921 gggcgatggg cgtgatggat cagaaattcg gtagcgcagc agcagcttac agctacctga
    40981 ttttcgtcct gctgtatgta ccatgtatct cggtgatggg agccatcgcc cgtgaatcaa
    41041 gccgtggctg gatgggcttc tccatcctgt gggggctgaa tatcgcttac tcgctggcaa
    41101 cattgttcta tcaggtcgcc agctacagtc agcacccaac ttacagcctg gtgtgcattc
    41161 tggcggttat cctgtttaac atcgtggtta tcggtctgct gcgccgcgcg cgtagccggg
    41221 tggatatcga actgctggca acccgcaagt cggtaagcag ttgctgcgca gccagcacca
    41281 ccggtgattg ccattaatgg cttcacttat tcaggtgcgt gatttgctgg cgttacgggg
    41341 ccgtatggaa gcggcccaga taagccagac attgaacact ccgcagccaa tgattaacgc
    41401 catgctgcaa cagctggaaa gcatgggcaa agccgtgcgg attcaggaag aacctgacgg
    41461 ctgcctctct ggcagttgta aaagctgccc ggaaggcaaa gcctgtctgc gcgagtggtg
    41521 ggcgctgcgt taaccatact ccatcgcctg ttttgaaaag cccggtatgc gtttgcatcc
    41581 gggctttttt gcgtgcggct ttccataaaa atgcaaccct tgcagtacaa cgtaagttcc
    41641 tctgaaagcg tctcgcaggg atgaaaactc gctaatgcac aggtgtggag tggcgcgtag
    41701 agtcacggca ttcaaacaac aggtgaagga acgccatgag caaaaagcag agttccaccc
    41761 cacatgatgc actattcaaa ctctttttac gccaaccgga cacggcacgc gattttcttg
    41821 cgttccattt accggcacct attcacgcgc tctgtgatat gaaaaccctc aagctggagt
    41881 cgagcagctt tattgatgac gatctgcgtg aaagctattc cgacgtgctg tggtcggtga
    41941 aaacggaaca aggaccggga tatatctatt gtctgattga acatcaaagc acctcaaaca
    42001 aactgatcgc atttcgcatg atgcgttacg ctattgccgc aatgcaaaat cacctggatg
    42061 ccggatacaa aacgttgccg atggtggtgc cattgttgtt ttaccacggt attgaaagcc
    42121 cctatcccta ttcgctgtgt tggctggatt gtttcgccga tcccaacctg gcaaggcagc
    42181 tttatgcctc cgcatttccg ctgattgatg tcaccgtcat gcctgatgat gaaatcatgc
    42241 agcaccgacg tatggcgctg ctggagttaa ttcaaaaaca tattcgtcaa cgcgatctga
    42301 tggggctggt agagcaaatg gcctgcttat taagtagtgg atacgctaat gacagacaaa
    42361 tcaaagggct gtttaattac atcttgcaaa ccggcgatgc ggtacgtttt aacgatttta
    42421 tcgacggcgt tgccgaacat tcaccgaaac acaaggagag cttaatgact attgcggaaa
    42481 gattgcggca ggaagggttt cagaaaggta tacgtaccaa cgccctaaat attgccaaaa
    42541 ccatgctgga cgccggagtt tctcttgagg acgttctgcg atttaccagc ctgacggttg
    42601 aagaccttgc tgaaataaat catcaataac gtaaaggatg gttttaatga cagatgcaga
    42661 aagagaatat cttattagtt acctgattgg ttatttattg ggtcgacgcc tggaagccct
    42721 ggatatcgcc aaaagaatgc tgtaatccgg agtccctctt gcggacatca tgcgctttac
    42781 cggggtgtca gaagaagagt tggctgcggc gagtcagtaa aattctgtct cgccatttca
    42841 aaagccacct acaccctctg cttcaacgcc accagcacgt gacgaaactc gaccggatgc
    42901 gaaataaacg gcgcatgggc cgctttggcg aaaatgtacg actcgctgtg cggccagagt
    42961 ttatccagca tcggcaccac tttgcgcggc accagaccat cgagatagcc atacaatcgc
    43021 aaaaacggca tggacacgtt ttgcagcggc tgacggagat cgaccgtttt caggatttcc
    43081 agcccgccat taagcacgtc aacctccggc atcggtaacg ccagaacggt tttcttcagc
    43141 gcccgcgcat cctggcgcgc cgtttcagta cccatggttt gtaacgcaag gaaccgttcc
    43201 actgtacgct gaaaatcatc actgagttgc tgctgaaatc ccgccagcac gtccggtttt
    43261 atccccggcc actcgtcacg agcactaaaa caaggcgacg acgccacggt gaccagcgcc
    43321 tgaacacgct cgggatgggt taacgcaatc tggcttgcca ccagcccgcc cagactccag
    43381 cctaaccaaa tggctttatc aggtgcctgt cgcagcacgg cttcggccat atcagcaagt
    43441 gacatcgcac caaatccccg gctacgcccg aagccgggca ggtcaacaag gtgcagcgta
    43501 aaatgcgagc tcagttcctc gtcaatgcaa cgccacactt cggcattcag tccccatccg
    43561 tgcaacagca caagatgaac attcccctga cctttggtct gccaccagat gttattcatc
    43621 cgctattgtt ctctttttac ttacaaggat gaacatatgc taacagtacc gggattatgc
    43681 tggctatgcc gaatgccgct ggcgttaggt cattggggga tttgttcggt atgctcacgc
    43741 gccgcccgta cagataaaac gttatgcccc caatgtggat tacccgccac acactcacat
    43801 cttccctgcg gtcgctgcct gcaaaaaccg ccgccctggc aaagactggt cacggttgcc
    43861 gactatgcgc cgccgttaag tccgcttatc caccagctta agttttcccg gcgcagcgaa
    43921 atcgccagcg ccctggcacg tctgttacta ttggaagtct tacacgctcg tcgcaccacc
    43981 gggttgcaat tgccggatcg cattatcagc gttccgttat ggcagcggcg tcactggcgt
    44041 cggggattta atcagagcga tttgctgtgt cagccgttat cacgctggtt gcactgccga
    44101 tgggatagcg aagccgtcac acgaacacgg gccactgcga cccagcattt tctcagtgcc
    44161 cggctgcgca agcgcaacct gaaaaatgcc tttcgccttg aattgcctgt gcaaggtcgc
    44221 catatggtga ttgtggatga tgtcgtcacc accggaagta ccgtcgcaga gattgcgcag
    44281 ttgcttttac gcaatggtgc ggcgactgtc caggtctggt gcctttgtcg aaccttgtag
    44341 agcctcgatg atgggcgtat tataaccaac taaaatagtc aactattagg ccattactat
    44401 gatccgtatt tccgatgctg cacaagcgca ctttgccaaa ctgctggcaa atcaggaaga
    44461 agggacacaa atccgcgtat ttgtgattaa ccctggcacg cctaacgctg aatgtggcgt
    44521 ttcttattgt ccgccggacg ctgtagaagc caccgacact gccctgaaat ttgacctgct
    44581 gaccgcgtat gttgatgagt taagcgctcc ttacctggaa gatgcagaga tcgactttgt
    44641 tacagaccag ttgggttccc agttaacgct gaaagcgcct aacgccaaaa tgcgtaaagt
    44701 cgcagacgat gcaccgctga tggagcgcgt ggagtatatg ctgcagtcgc agatcaaccc
    44761 acagcttgct ggtcacggtg gtcgcgtttc gctgatggaa atcaccgaag acggttacgc
    44821 cattctgcaa tttggcggcg gctgtaacgg ttgttctatg gtagatgtga cgctgaaaga
    44881 agggatcgag aagcagctgc tgaacgaatt cccggagctg aaaggtgtgc gcgatctcac
    44941 cgaacaccag cgcggcgagc actcctacta ctaagttatc ccctcttttg tagattgccc
    45001 gatgcgacgc taaagcgtca tttcgggcct acgttgaaca tgtgccagac gttggcgcag
    45061 cattgctttg gcaaccccct cccttcgttt ccccgcgata atatgaccaa cctctcataa
    45121 tttaaattta ccccgctctg gtgattctca aacgccggat gttacccgta tcattcacgt
    45181 gggtaccaaa catactcctg acatctgact acaataatta gttttagtgg gtatcagtcg
    45241 tggtgccgca atatctctgt tcccgattgg gataattaga gtttgtcgtc agaaaattga
    45301 cgttacccat aacaaatgaa aggccaggta aatcatgcca ttagtcattg ttgctatcgg
    45361 tgtaatcttg ttgttgctcc tgatgatccg tttcaaaatg aacggcttca tcgctctcgt
    45421 cctcgtggcg cttgctgttg gattaatgca aggaatgccg ctggataaag ttattggctc
    45481 catcaaagca ggtgtcggcg gaacgctcgg tagccttgcc ctgatcatgg gttttggcgc
    45541 aatgctgggc aaaatgctgg cagactgcgg tggcgcacaa cgtatcgcca ccacgctgat
    45601 tgctaaattt ggtaaaaaac acatccagtg ggcggtggta ctgaccggtt ttaccgttgg
    45661 ttttgccctg ttctatgaag tgggtttcgt gctgatgctg ccgctggtgt ttaccatcgc
    45721 ggcttccgcg aatattccac tgctgtatgt tggtgtacca atggcggctg cactgtctgt
    45781 gacccacggc ttcctgccac cgcatccggg cccgactgcg attgccacca ttttcaatgc
    45841 cgatatgggt aaaaccctgc tgtacggtac tattctggca atcccgaccg tgattctcgc
    45901 cggtccggtt tacgctcgcg tgttgaaagg tatcgataag ccaattccgg aaggtctcta
    45961 cagcgcgaaa accttcagcg aagaagagat gccgagcttt ggcgtcagcg tctggacctc
    46021 tctggtgccg gtagtactga tggcgatgcg tgcgattgcc gaaatgatcc tgccgaaagg
    46081 tcacgctttc ctgccggtag cggagttcct cggtgacccg gtaatggcta cgctgattgc
    46141 cgtactgatt gcgatgttca cctttggtct gaaccgtggt cgttcaatgg atcagattaa
    46201 cgacacgctg gtttcttcca tcaaaatcat tgcgatgatg ctgttgatta tcggtggtgg
    46261 cggtgcgttc aagcaggtgc tggtagacag cggcgtggac aaatacattg cttccatgat
    46321 gcacgaaacc aacatttctc cgctgctgat ggcctggtcg attgctgccg tactgcgtat
    46381 cgcgctgggt tccgcaaccg ttgcggcaat cactgcgggt ggtatcgcgg caccgctgat
    46441 tgcaacgacg ggtgttagcc cggagctgat ggttattgcg gttggttccg gtagtgtgat
    46501 tttctctcac gtgaacgatc cgggcttctg gctgttcaaa gagtacttta acctgactat
    46561 cggcgagacc atcaaatcct ggtcgatgct ggaaaccatt atctcggtgt gcggtctggt
    46621 aggctgtctg ctgctgaata tggtgatttg ataacactga ctgccggatg catcgcatcc
    46681 ggcatcaacc gcactctact tcttcttcgc tgccgctctg cgccgtctgt ccaaatcctt
    46741 cagcaacttg ttcacaccat catcggcaaa catcgcctca agcgttgcgg agagcttgcg
    46801 ccgccagttt ttatactggt aactggtgcc gggaatattt accgggtccg ccatatccag
    46861 ccagtcttct ggctgtagtc ctaacagggc gctgttgcta tcggcgatgt agcgctgtaa
    46921 gccacgattc agcgtcggtg tcatcgacat caacgatgcc ttatgcccgg cacgttttgg
    46981 caggcaacca tatttatgca gagcatccag cagcccttgc ttcgccagtt cgcgatcctg
    47041 atacagaccg cgcagtacca cttcatccgg atacagcccc agcgttttgc ccagcgttaa
    47101 atccccgctc tcccagtagc cgcgtagcgt aggcaggtcg tgtgtcgccg caaccgccat
    47161 cgactgctct gggtacgctt tcggtgcgcg gaatgtcttc tcgtggtcgt tttcgaaata
    47221 gagcactttg taggagtaca caccgctgct gcgcagctta ccgacaatct ctaccggtac
    47281 ggtaccgaga tcttcaccaa tcaccataca gcgatgacgt ttactttcga gtgccagaat
    47341 cgagagcaga tcatccaccg gatagtgaac atacgcgccc tgatctgccg tctcgccata
    47401 cggtatccac cacaaacgca gcatcgacat cacgtgatca attcgtaatg cgccgcagtt
    47461 ttgcatattg gcacgcaaca gctcgataaa cggttcatag gcacgcgcgg tgataatatg
    47521 cgggtccatt ggcggcaatc cccagttctg ccccaacggg ccgaggatat ccggcggcgc
    47581 gccaaccgac gctttcaggc aatacagttc acggtcacac caggtttccg ccccaccttc
    47641 cgctacgcca accgccagat cacgatacaa gccaatcggc atttcatagc cctggcttat
    47701 ctcccagcag gcggcaaact ggctgtaagc cagccactgc aaccagagat aaaaatcgac
    47761 gtcatcacga tgttcttcgc agaactggcg cacttctggt gaatccacgt tctgatacat
    47821 ctctggccat gctggccagc cccagcgcat ttcgtcctct ttcacttgct gggcatgtag
    47881 cgcatcaaag gctgcctgcc agaacaggct gtcgccctgc tctgcaacaa actggcgaaa
    47941 cgcggtcatc tgctcatcat cacgttgcgc gaaacctttc cacgccattc gtaatgctgt
    48001 catttttagg gcggtaaccg tggagtaatc aacccagtcg gcatcccgcg cctgttgcag
    48061 cgtctgttgc gtggtcggca actgccacca ggcctgagcc tcttcgctaa gatggaaatc
    48121 ttcaactgcg ttaacgtcga tataaatcac attcagccaa cggcgagaag acgggctata
    48181 cgggctggca ctttccggat ttgccggata gagcgcatga atcgggttca gaccgatgaa
    48241 cgacccgcca cgttttgcca catcctcaag catcgctttg agatcgccaa aatccccaat
    48301 accccagttt ttttccgatc gcagcgtata aagctgaacg caggcacccc acagcttttg
    48361 tttattcagc aacgcctgcg gttcgtaaca gcgtttcggg gcgacaatca cccggcaatg
    48421 cgcgcgctgg tcgtcctggg tgagtgtcag cgtgtgataa ccttccggca gcttcgtcgg
    48481 tagattgaac gctttgcccc ccgttacatg gcctttgtac tgcgttcctt cttcggtggt
    48541 cagcagccag ctatattcgc cgctgccctc caccaccatc ggcatttttt tgccgctggt
    48601 ataaaccatg acattcggga ctggcgttac cgccactttc gtggcggtac gttgatgcat
    48661 cgcgtcaagc aaacgccgtt tggtttcggc gctaatcgac tgcggtttac cgtgggcatt
    48721 gatgtaattg gggctaatcc ccgccgccag cgcggcatta tccagacgtt tgctttccat
    48781 cgagcttcct tagcgttttg cctgccagat acgagcctga taatcgcgaa tagagcgatc
    48841 cgagctaaac ataccgcagc gggcggtatt gaggatcgcc gcgcgagtcc aggcctcctg
    48901 gtcgcggtac agcacatcca cctgcttttg tgcctctaca taagcggcga aatccgccat
    48961 caccagatac ggatcgccgc cctgtttgcc gatactgtgc agcatctggt cgaaggcatg
    49021 cttatcgccg tcgctgtatt taccgctttc cagctctttc aatactgcgt ccagcacctt
    49081 atctttcttc cgccatttca ccgggtcgta gcctttggcc agaattgcct tcacttgttc
    49141 cacggtatga ccaaaaataa agatattttc ttcaccaact ttctcggcga tttcaacgtt
    49201 cgcaccatcc agcgtaccga cagtaagcgc accattgagc gccagtttca tattgccggt
    49261 accggaagct tctttacctg ccgtcgaaat ttgttcggag atatccgccg ccgggatcag
    49321 tttttccgcc gccgaaacgc aataatccgg caggaacacc accttcaatt tgtcgccaac
    49381 ctgcggatcg ttgttgatca cgtcagccac tttgttgatc gcaaagataa tgtttttcgc
    49441 caggtagtag cccggtgccg ctttcgcgcc aaagaggaag acgcgcggta cgcgatcagc
    49501 ctgcgggttt tcacgaattt ctttgtacaa cgccagaata tgcagcagat tcaggtgctg
    49561 gcgtttgtac tcatgcagac gtttgatctg aatatcgaaa atcgcctgtg gattgatctc
    49621 aataccggta cgaactttca caaactctgc cagacggact ttattcgcct gcttgatctc
    49681 gcgatattgc tgacggaatt tcgcatcatc agcgaatttt tccagattga tcagttgatc
    49741 gagatcgtta gcccactctt ttttcagtga tttatccaac agagccgcca gtgccgggtt
    49801 gcactgtttg atccagcgac gtggggtaat accgttggtg acattatgga atttgttcgg
    49861 ccatagctgg tgatattccg ggaacagatc tttcaccacc agatctgagt gcagcgccgc
    49921 aacaccgttc accgcgaaac cgccaaccac acacaggttc gccatatgca cttgtttgtc
    49981 gtgcaccacc gccagtttgg cccacacttt ttcatcgccc ggccaggttt tctccaccag
    50041 cgttttaaag cgagtattaa tttcgttaat aatctgcatg tggcgcggca gtaagccttt
    50101 caccagtttc acatcccagc gttccagcgc ttctggcatc agggtatggt tggtgtaagc
    50161 gaaagttttg ctggtgatgg cccaggcgtc atcccagctc atctggtgct catcgatcag
    50221 cacgcgcagc agttctggaa tggcgatagt tgggtgggta tcgttcagct gaataacttc
    50281 gtaatccgcc agttcgtgca gtttacgccc cgccagatga tggcgacgca aaatatccgc
    50341 taccgagcag gcacactgga agtattgctg catcaggcgc agctttttac cggcagtatg
    50401 gttgtcgttt ggatagagaa ctttggtcag tttttccgcg ttgatgccct gctgttcggc
    50461 acgcaggaaa tcaccatcgt taaatttagt cagatcaaac ggatgcgcat gcgtcgcctg
    50521 ccacagacgc agtggctgcg ccacgccatt acgatagccg acaacgggga gatcccacgc
    50581 ttgaccggta atggtaaact ccggctccca gcgtccatct ttcgtcactt taccgccaat
    50641 ccctacctgc acatccagtg cttcgttgtg gcggaaccac gggtagttac cgcgatgcca
    50701 gtcatccggc gcttcaacct gtttgccatc gacaaaagac tggcggaaca agccatattg
    50761 ataattaagg ccgtagccag tagctgactg cccgacagtt gccattgagt cgaggaagca
    50821 cgccgccaga cgtcccagac caccgttacc cagcgccggg tcgatctctt cttccaacag
    50881 gtcagtcagg ttgatgtcat aagccttcaa cgaatcctgt acatcctgat accagccgag
    50941 attcaacagg ttgttgcccg tcaggcgacc aattaaaaac tccattgaga tgtagttaac
    51001 atgtcgctga ttcgccaccg gtttggcaaa tggctgagca cgcagcattt cggccagtgc
    51061 ttcgctcact gccaaccacc actgacgagg ggtcatttca gccgcagaat ttaagccata
    51121 acgctgccac tgacgtgaaa gcgcttcctg aaattgctta tcgttaaaaa taggttgtga
    51181 cataggagtt ccacttttct tagattttca acacaacgtt atcgctagtt tgccaggctc
    51241 gatgttgacc ttcctcatcc tgcgggggat taggcaggga ggagttgtgg ggatgagcaa
    51301 ggaaatgtga tctcgaccac ttaaagctag tgcaaaccac aggattagca gcaaatcaat
    51361 gctatacagc gcagaaaatc tgtatctaag tgcaaaaaat ggccgttgcg tattttcaaa
    51421 aagcggaagg taactctata aattaagtaa aggagtgaaa cagtttcaca agtaaaatat
    51481 ccagtgtgct ccatctcatt cttaatagat ttattaagat catcttttta gatggcactt
    51541 tcatcagaaa tgaagaagaa acccttgctt aaatgaaact gatgaacata aggaaaatac
    51601 cgtacaaccc agtattcacg ctggatcagc gtcgttttag gtgagttgtt aataaacatt
    51661 tggaattgtg acacagtgca aattcagaca cataaaaaaa cgtcatcgct tgcattagaa
    51721 aggtttctgg ccgaccttat aaccattaat tacgaagcgc aaaaaaaata atatttcctc
    51781 attttccaca gtgaagtgat taactatgct gattccgtca aaattaagtc gcccggttcg
    51841 actcgaccat accgtggttc gtgagcgcct gctggctaaa ctttccggcg cgaacaactt
    51901 ccggctggcg ctgatcacaa gtcctgcggg ctacggaaag accacgctca tttcccagtg
    51961 ggcggcaggc aaaaacgata tcggctggta ctcgctggat gaaggtgata accagcaaga
    52021 gcgtttcgcc agctatctca ttgccgccgt gcaacaggca accaatggtc actgcgcgat
    52081 atgtgagacg atggcgcaaa aacggcaata tgccagcctg acgtcactct tcgcccagct
    52141 tttcattgag ctggcggaat ggcatagccc actttatctg gtcatcgatg actatcatct
    52201 gatcactaat ccagtgatcc acgagtcaat gcgcttcttt attcgccatc aaccagaaaa
    52261 tctcaccctg gtggtgttgt cacgcaacct tccgcaactg ggcattgcca atctgcgtgt
    52321 tcgtgatcaa ctgctggaaa ttggcagtca gcaactggca tttactcatc aggaagcgaa
    52381 gcagtttttt gattgccgtc tgtcatcgcc gattgaagcc gcagaaagca gtcggatttg
    52441 cgatgacgtt tccggttggg cgacggcact gcagctaatc gccctctccg cccggcagaa
    52501 tacccactca gcccataagt cggcacgccg cctggcggga atcaatgcca gccatctttc
    52561 ggattatctg gtcgatgagg ttttggataa cgtcgatctc acaacgcgcc attttctgtt
    52621 gaaaagcgcc attttgcgct caatgaacga tgcactcatc aaccgtgtga ccggcgaaga
    52681 aaacgggcaa atgcgcctcg aagagattga gcgccagggg ctgtttttac agcggatgga
    52741 tgataccggc gagtggttct gctatcaccc gctgtttggt aacttcctgc gtcagcgctg
    52801 ccagtgggaa ctggcggcgg agctgccgga aatccaccgt gccgccgcag aaagctggat
    52861 ggcccaggga tttcccagcg aagcgattca tcatgcgctg gcggcaggcg atgcgctgat
    52921 gctgcgcgat attctgctta atcacgcctg gagtttgttc aaccatagcg aactgtcgct
    52981 gctggaagag tcgcttaagg ccctgccgtg ggacagtttg ctggaaaatc cgcagttagt
    53041 gttattgcag gcgtggctga tgcaaagcca acatcgctac ggcgaagtta acaccctgct
    53101 agcccgtgct gaacatgaaa tcaaggacat cagagaaggc accatgcacg cagaatttaa
    53161 cgctctgcgc gcccaggtag cgattaacga tggtaatccg gatgaagcgg aacggctggc
    53221 aaaactggca ctggaagagc tgccgccggg ctggttctat agccgcattg tggcaacctc
    53281 ggtgctgggt gaagtgctgc actgcaaagg cgaattgacc cgctcactgg cgctaatgca
    53341 gcaaaccgaa cagatggcac gccagcacga cgtctggcac tacgccttat ggagtttaat
    53401 ccagcaaagt gaaattctgt ttgctcaggg attcctgcaa accgcgtggg aaacgcagga
    53461 aaaagcgttc cagctgatca acgagcagca tctggaacag ctgccaatgc atgagtttct
    53521 ggtgcgcatc cgagcacaac tgttatgggc ctgggcacgg ctggatgaag ccgaagcgtc
    53581 ggcgcgtagc gggattgaag tcttgtcgtc ttatcagcca cagcaacagc ttcagtgcct
    53641 ggcaatgttg attcaatgct cgctggcccg tggtgattta gataacgctc gtagccagct
    53701 taaccgtctg gaaaacctgc tggggaatgg caaatatcac agcgactgga tctctaacgc
    53761 caacaaagtc cgggtgattt actggcaaat gaccagcgat aaagccgccg ctgccaactg
    53821 gttgcgtcat acggctaaac cggagtttgc gaacaaccac ttcctgcaag gtcaatggcg
    53881 caacattgcc cgtgcacaaa tcttgctggg cgagtttgaa tctgcggaaa ttgtcctcga
    53941 agaactcaat gaaaatgccc gcagtctgcg gttgatgagc gatctcaacc gtaacctgtt
    54001 gctgcttaat caactgtact ggcaggccgg acgtaaaagt gacgcccaac gtgtgttgct
    54061 ggacgcatta aaactggcga atcgcaccgg atttatcagt cattttgtca tcgaaggcga
    54121 agcgatggcg caacaactgc gccagcttat tcaactcaat acgctgccgg agctggaaca
    54181 gcatcgcgca cagcgtattc tgcgagaaat caatcaacat catcggcata aattcgccca
    54241 tttcgatgag aatttcgttg aacgtctgct aaatcatccg gaagtgccag agctgatccg
    54301 caccagcccg ttgacccaac gtgaatggca ggtcctgggg ctgatttact ccggttacag
    54361 caacgagcaa attgcaggtg agctggaagt ggcggcaacc accatcaaga cgcatatccg
    54421 caatctgtac caaaaactcg gtgtcgccca ccgtcaggcc gcggtacaac acgcgcagaa
    54481 actgctgaag atgatgggat atggggtgtg agtttagccg gataatacgt cggatccggc
    54541 ttacatcttt atatcattca atgctcaccc gcgttacgcc atccgcctcc accagactaa
    54601 accgcactgg caagaaacgc tccaccaccg caatattggt cagcagatgg catgagggat
    54661 gggcgaccgt aaattctccc gcgcccgcca gcgccatcgg cagtaccagc tggtcggcga
    54721 gatattcccc caccgccgcc ggacttgcca ggtagcgttt cacctctttc accaactgtg
    54781 ccgcgaccac ttcggcgctg acgcgctttt caccgacgac aaaaaagcgt tcggtgatat
    54841 tttcactttc gacttcaagc gaaacggtat tacccggtcc ctggtcacgc ggcaggttat
    54901 gaatattctg ctcatgcagg gaaaaactcc ccgccagtgt agcgatttca cgctcagcga
    54961 catggcgcgg cacgccagct aatagcacct ctccacgcat ctgcacaatg ttcccgcgct
    55021 cgccaagttg caaggtgtta aacaatgcca ccggcgagac ttccgttgcc accaccccgc
    55081 ctccagcagg ataaaaaccg tggcgtaata atgtggtttg ctgatgaatg cccattttcg
    55141 ccagcagcgg ctccagcacc cggcggataa aatccgcagg cggtgccgac gggttatcgg
    55201 tgccgccgct cacttcaaca cgcgaaggcc catcagcaaa ccacagcgcg ggtagcaccg
    55261 tttgcagcac cagcgtgctg cttccggcac taccgatagc aaagcggtaa tcgccgccac
    55321 gcacggtacc gggccggaag accagacgct gcgaccccag ctccgcgcct tccaccgttg
    55381 ctcggcaaat ttccgtcgcc gccttaaccg cggtcagatg ctggcgcaac agccccggtt
    55441 tcgcccgccc ggcacgaatg ccggtgatgg taaatggctg gccggttatc atagacaggc
    55501 tcagcgccga gcgcaggatc tgcccgccgc cttcgccctg tgcgccgtcc agcgcaatca
    55561 tccttttcat catttatcct tttacgcaca ccacctgacg cagggtgtag ataacttcca
    55621 ccagatcgct ttgtgccgcc atcaccgcat caatatcttt atacgccatc gggatttcgt
    55681 cgatcacttc ggcatcttta cggcattcca catgcgcggt ggcacgaatt tgatcttcca
    55741 cgctgaacat ttttttcgct ttagtacggc tcataacccg cccggcaccg tggctgcacg
    55801 aacagaacga ctcttcattt cccagcccac ggacgataaa gcttttcgct cccatcgaac
    55861 cgggaataat tccatattga ccagcacgcg cagacaccgc gcctttacgc gtcacgtaga
    55921 tctcttcacc aaagtgctgt tctttttgca catagttgtg gtgacagttg atctcttcca
    55981 tcgccagcgt ttgtggctgt ctgaccgttt tctgcgtaat gctctgcaac gccgttacca
    56041 cgttttccat catcgcatcg cggttaaggc tggcaaaaag ctgcgcccag gccacggctt
    56101 tcaggtaatc atcaaagtat tccgtacctt ccataaagta cgccagatca cgcgacggca
    56161 atgtttcaag cgtttcctgc atctcttttt gcgccagatc gataaagtaa gtcccgatgg
    56221 cgttaccaat tccgcgtgaa ccggagtgca gcataatcca cacctggtcc gactcatcaa
    56281 ggcagatttc aataaagtgg ttacccgttc ccagcgttcc caggtgttta tagttgttag
    56341 tgttaaggaa acggggatat ttttgcgtta accactgata accggcttcc agctcagccc
    56401 atttcgcatc gacgttaaca ggcggatttt cccatgcgcc tttatcacgt ttacaacgtc
    56461 cggtagtacg tccgtgcggc acggctgttt caatcgcctg acgcagctcc gccaggtttt
    56521 ccggcaggtc tgccgccgtt aacgcggtac gcagcgcgtt cattccacaa ccgatatcca
    56581 cgcccaccgc cgccggaata atcgcccctt tggtcgggat cacgctacca atggtagaac
    56641 ctttacccag gtgtacatcc ggcattaccg caatatgttt gaaaataaac ggcatcttcg
    56701 ccgtattaat aagttgctga cgcgcatcgg cctctaccgg cacgcctttg gtccacattt
    56761 ttaccggggc attttcagtg gttagtaatt cgtaattcat tttttttctc ttttcgttgt
    56821 ttgctgtcct gataattgca accgtc
//
LOCUS       DAPHCV010000012        54406 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.012, whole genome shotgun sequence.
ACCESSION   DAPHCV010000012 DAPHCV010000000
VERSION     DAPHCV010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.012"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            98..1480
                     /locus_tag="RH033_000749"
     CDS             98..1480
                     /locus_tag="RH033_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HDV2989981.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTIVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGFLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            1490..3808
                     /gene="yicI"
                     /locus_tag="RH033_000750"
     CDS             1490..3808
                     /gene="yicI"
                     /locus_tag="RH033_000750"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HDV2989982.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLHNGERITGSQVKNNGYVQDTNSQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSEPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDSTLLALGNNDQRPDYAW
                     HEGTAFHLFNLQDGHEAVCEVPDADGSVIFTLKAARTGNTITVTGTGEAKNWTLCLRN
                     IVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            4103..5656
                     /locus_tag="RH033_000751"
     CDS             4103..5656
                     /locus_tag="RH033_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDV2989983.1"
                     /translation="MQMITSRQNRLLRFLLPRREYTTIVTIAGYLNVSEKTIQRDLRL
                     LEQWLGQWRINVEKRAGAGVMLSAENIADLLHLDHLLVAECEEIDGVMNNARRVKIAS
                     QLLSETPNETSISKLSERYFISGASIVNDLRVIESWLAPLGLSLIRSPSGTHIEGSEG
                     QVRQAMALLINGIINHNEPQGVVYSRLDPGSYKALVHYFGEEEVLFVQSLLLDMENEL
                     SWSLGEPYYVNIFTHILIMMYRNTHGNALSREEDQTRQYDENIFNVASQMIHKIEQRI
                     AHTLPDDEVWFIYQYIISSGVAIDGQKDVSIISHMQASNEARLITWRLITVFSDIVDC
                     DFSEDSALYDGLLVHIKPLINRLNYRIHIRNPLLEDIKAELADVWRLTQYVVNQVFKT
                     WGENAVSEDEVGYLTVHFQAAMERQIARKRVLLVCATGIGTSHLLKSRILRAFPEWTI
                     VDVISAANLSQVLPDNIELIISTINLPTVTMPVAYVTAFFNDADIKRVTEMVITEKLH
                     HATSRVVEI"
     gene            5683..6153
                     /locus_tag="RH033_000752"
     CDS             5683..6153
                     /locus_tag="RH033_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007370547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDV2989984.1"
                     /translation="MDITKILNTNRVILDMQATNKEEAIEELTNLLKKDGAIDCRETF
                     IKDVWQREAEGSTGFENHIAIPHGKSSAVINTTLAIGRTRQDIPWETLDGSSVRCIIL
                     FAVRLEDQNTTHIRLLSQVAGALADDDIIEQLLVETSPQKIIDLFSQYAQSDVC"
     gene            6171..6485
                     /locus_tag="RH033_000753"
     CDS             6171..6485
                     /locus_tag="RH033_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDV2989985.1"
                     /translation="MNIVCVAACTAGIAHTYIAREKLIKGAKALGHTIKVETQGTIGT
                     ENELQAADISAADVVILAVDVKIKGEERFTNKRIVRVKTEIVIKSPVQFLEKVEKSLG
                     NK"
     gene            6510..7601
                     /locus_tag="RH033_000754"
     CDS             6510..7601
                     /locus_tag="RH033_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDV2989986.1"
                     /translation="MKDNKIPVSQEIKKHLLTGISWMIPLIVAAGICIALGQVLGGTD
                     VGEKTGTIPWMLNQIGGWGMGLIVPLISAAIAYSIADRPGFAPGLIVGFLCGQIHTGF
                     IGGMLGGFLVGYTILLLKRYIRLPQSMQGLMPIMVLPVLSTIIGGLLMMTLIGKPIAW
                     LQEALIHLLESMQGGSRFLMGAILGAMATFDFGGPVNKTMSLFSDGLLVSGVYGPEAV
                     KFVGSIIPPFGITLSFLLTRHKYTRAEREALKAAFPMGICMITEGVIPIAARDLLRVV
                     GSCVVASAVAGGLIMVWGVESPVPHGGMFVVPLFTHPLLFCLALGIGTVICGVMLSLW
                     KKPVTERDEEFDELSDQKLKDEEITFTLE"
     gene            7613..8464
                     /locus_tag="RH033_000755"
     CDS             7613..8464
                     /locus_tag="RH033_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HDV2989987.1"
                     /translation="MFADMKSMVMKAWHEHYALLAINCMNLESAHAAIRVAEKNRAPI
                     ILNLYQGHLAHFPAPVAAAVVKTLAESASVPVTLALDHGKNPDCIRQAFRAGFSGLMI
                     DASAFPLEENVRQTRAVVELAASAQLCVEGELGHLADAPRYDQAANADLMTQPTDVET
                     FIAQTGIDLLAVSVGTAHGMYAPGVVPAIDFQRLAEISRCSSVPLALHGGSGTPFDQL
                     QLCTTLGVAKINVGAAIFERGKSALLHTLCHDISIELVDALKAMELAFEEAITPYLQA
                     SGSIGKA"
     gene            8545..9405
                     /locus_tag="RH033_000756"
     CDS             8545..9405
                     /locus_tag="RH033_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDV2989988.1"
                     /translation="MLVSMKELLQPTRQHGFAIGAFNVADNCFLRAVVDEAEATNTPA
                     IIAIHPSEHDFVGDAFFAHVREITQRSPVPFVLHLDHGASVEHVLRAIRCGFTSVMID
                     GSLLPYEENVALTAEVVRLAHAVGVSVEGELGTIGQTGTSVEGGVSQVTYTDPAQAAD
                     FIARTGADTLAVAIGTAHGIYPKGMQPKLQMDILRDIAGRIDIPLVLHGGSANPDAEI
                     AESVTLGVGKINISSDMKYAYFQKVREILAKESWWDPNVIYPDAINAAREVIRHKMKL
                     FGSLGKASLY"
     gene            9453..10385
                     /locus_tag="RH033_000757"
     CDS             9453..10385
                     /locus_tag="RH033_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDV2989989.1"
                     /translation="MHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCV
                     VALIEQIRRDVQRPMLTGIALPGSISPLTGLIKNANIQVINGHALQADLQQLLGQPVV
                     IANDGNCFALSEACDGAGQDYDVVFGITLGTGCGGGIAIKQRPFIGAWGNAAECGHIT
                     LPGYTEQEDGPSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTPAAIVTLAQRGDA
                     CAMQQVARFRQQLARTLATIVNVVDPGVIVIGGGLSNVELLITDLNAEVAPLVFTDQF
                     TTPIVKAQHGDSSGMRGAAWLAMRNGEANETFTN"
     gene            10366..11046
                     /locus_tag="RH033_000758"
     CDS             10366..11046
                     /locus_tag="RH033_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HDV2989990.1"
                     /translation="MKRSQINYVIDKAHAIAQTFRVCLPEFAYFTVDDWLQRERDNWQ
                     EVVDLQLGWDITDFGRGDFNQTGLTLLTMRNGALGSAAYPKPYAEKMLQIQQDQQTPW
                     HFHTHKMEDILNRGGGDLCMQLAWANEASLCDDQRVITVSVDGQRRTMKPGETLVLKP
                     GQGICLPPRLYHRFWAEKAFVLGWEISMVNDDQHDNYFLEPGGRFPAIEEDEPVKWLL
                     CSEYAVLR"
     gene            complement(11089..12798)
                     /locus_tag="RH033_000759"
     CDS             complement(11089..12798)
                     /locus_tag="RH033_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HDV2989991.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRLADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVADLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWQRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQHWGWPEL
                     PLTGDGNIQLTASGDIQANIPLKPTVSGQLHAMNATKQQVTQTMNAGVVSSGEVTSTE
                     PVQ"
     gene            complement(12919..14310)
                     /gene="xanP"
                     /locus_tag="RH033_000760"
     CDS             complement(12919..14310)
                     /gene="xanP"
                     /locus_tag="RH033_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HDV2989992.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            14589..15794
                     /gene="gltS"
                     /locus_tag="RH033_000761"
     CDS             14589..15794
                     /gene="gltS"
                     /locus_tag="RH033_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HDV2989993.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(15970..18051)
                     /gene="recG"
                     /locus_tag="RH033_000762"
     CDS             complement(15970..18051)
                     /gene="recG"
                     /locus_tag="RH033_000762"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDV2989994.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFNAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDAPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRNDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18057..18746)
                     /gene="trmH"
                     /locus_tag="RH033_000763"
     CDS             complement(18057..18746)
                     /gene="trmH"
                     /locus_tag="RH033_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HDV2989995.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADRDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(18753..20861)
                     /gene="spoT"
                     /locus_tag="RH033_000764"
     CDS             complement(18753..20861)
                     /gene="spoT"
                     /locus_tag="RH033_000764"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HDV2989996.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(20880..21155)
                     /gene="rpoZ"
                     /locus_tag="RH033_000765"
     CDS             complement(20880..21155)
                     /gene="rpoZ"
                     /locus_tag="RH033_000765"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDV2989997.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(21210..21833)
                     /gene="gmk"
                     /locus_tag="RH033_000766"
     CDS             complement(21210..21833)
                     /gene="gmk"
                     /locus_tag="RH033_000766"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDV2989998.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            22091..23776
                     /gene="ligB"
                     /locus_tag="RH033_000767"
     CDS             22091..23776
                     /gene="ligB"
                     /locus_tag="RH033_000767"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HDV2989999.1"
                     /translation="MKVWMAILISILCWQSSAWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGIYDQLSARLTQWQRCFGEETHRDAMMPPLNGAVIHPVAHTGVR
                     KMADKIALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVR
                     LISAVPQTVSGPLANSTLQGEIFLKREGHIQKQMGGINARAKVAGLMMRQDDSDTLNS
                     LGVFVWAWPDGPQLMTDRLKELATAGFTLTQTYTRAVKNADEVARVRNAWWKTKLPFV
                     TDGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVARVAEVKAIQFAVGKSGKISVVASL
                     APVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPT
                     PPENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRSLHQTHRFEHIF
                     SWLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDER
                     SWSQLLLSTEQFWQQLPGTGSGRVRQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(23773..24390)
                     /locus_tag="RH033_000768"
     CDS             complement(23773..24390)
                     /locus_tag="RH033_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HDV2990000.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(25120..25407)
                     /locus_tag="RH033_000769"
     CDS             complement(25120..25407)
                     /locus_tag="RH033_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HDV2990001.1"
                     /translation="MIRDRKAEELESKGLYRRAAARWAEVMMLADGDKEREHAANRRS
                     ECIRKAARPPATTDRFGDLRQAVKRTHSALGMDEEARGYFRRYRDKDCQRQ"
     gene            25667..26122
                     /locus_tag="RH033_000770"
     CDS             25667..26122
                     /locus_tag="RH033_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2990002.1"
                     /translation="MVFAMSKSNLVAFRIPSELQDEFNRSVLASGGDKTSWLVDAIRM
                     KLGQPEKSIDSRMLGLVERMEKAAASLIAGKPNIPPKPYNETAVIKIIADTIQQGFDN
                     GRVIAERINEAGYQTKAGKAWDKDIYSAWKRQGSNAEKLKAVIDCKVSV"
     gene            26202..26423
                     /locus_tag="RH033_000771"
     CDS             26202..26423
                     /locus_tag="RH033_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2990003.1"
                     /translation="MKGRTPHIEHQANHFSDVRNEMIAIRGDMHGLERRIIDKMDENR
                     DKASENQKWIIGLLILSILIPLLIALVTK"
     gene            26907..27296
                     /locus_tag="RH033_000772"
     CDS             26907..27296
                     /locus_tag="RH033_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2990004.1"
                     /translation="MIAILNKAKGRVGVNAQAVIMVFLGLIVAKLYPDWWKGALATVA
                     IYLLLSVVKRSFVKTVNQAKRFSFKNLINYITGLIFCLSILIASAFLPVSIANFFGAP
                     YEVGYLFSVFCLVLSLLYLWMRAKNKN"
     gene            complement(27315..29420)
                     /locus_tag="RH033_000773"
     CDS             complement(27315..29420)
                     /locus_tag="RH033_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="injection protein"
                     /protein_id="HDV2990005.1"
                     /translation="MAKAWKDVIASPQYQALAPEQKAQAQEQYFNEVVAPQAGESVEQ
                     AKQAFYAAYPLPSTNEIDRSQSATQNIQHTSSDNSLASGYAKLATQQREGLDRSAEQG
                     ASLGAAMRDAITGESRMTPEMERLKNVASAPELNSLSMDALKAGWSQLFGSDASQEKI
                     LQGMGATLRQDEKGNTIVSLPSGDYALNKPGLSPQDLTSFLANALAFTPAGRAGTVLG
                     AIGKSAATDLALQGATSLAGGEDIDPLQTVISAGIGGIGKGLENTASAVSRAVRGDMS
                     PEAKAAVDFASERNLPLMTSDMLKDKTFMQSQAQTLGERVPFFGTGKNRLNQQQAREN
                     LVRTFSDGLGGISDKQLYESATKGQQKFIEAAGKRYNRIIDAMGDTPVDLSNTVKAID
                     NQIAVLSRPGKSQDRAAVKVLQQFKDDITSGPNDLRLARENRTDLRKRFMASSDTVDK
                     DTLQKASDIIYKAYTADMKKAVAKNLGADEAINMARVDRSWSKFNDMMGRTRVQKAIA
                     SGKATPEDVTKLVFSQSPSERSQLYRLLDDNGRQNARAAIVQNAVDKATDPSGNISVE
                     KFINALHRNRKQSATFFKGVHGKELDGVIKYLNDTRHAAKANVQNLNGQQLYGLLVGG
                     GIINAAVLAGMLKTAAFVVPAAGAVGGAAKAYESPVIRNALLRLANTPKGSTAYDRAI
                     STVTQSLTRVAQASQKEAQ"
     gene            complement(29420..30886)
                     /locus_tag="RH033_000774"
     CDS             complement(29420..30886)
                     /locus_tag="RH033_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HDV2990006.1"
                     /translation="MATWQQGINSGGFLAGIGTQNENAPKASDINATLGLIRENNELA
                     RSGANNVALTGLRGLAGVADIYKQQQQQERKAAFQKGYADAYASGDREQMRNLITAFP
                     EEFEEVRKGMSYVDDAQRDDYGNLALKAQVASSLGQGAFGRFMMDNEQEMRRLGIPPE
                     TIAEMQVNDPHGFQHFAGNLALFSLGHEKYFDIKDQMEGRDIERGKLAETIRSNQAGE
                     ALQARGQDISRANALTSAYAPTAAMQNYNQYAQMLKADPEGAAAFAAAAGINTNAKKL
                     MSVRENDDGTVTKYYTDGSEEQGKLNQPISGDGFRPIALPTAQKIMEKSPEGAKKAAG
                     FAYRVRDALDSIDTLKDQLSPQRVAIINNALGNGTLANLTLSPTEQQYVVNANDAIMA
                     ILRQETGAAIAPAEMSKYYQMYFPQPGDSTKTIDTKRRKMENQFNSLKAASGRAYDAL
                     RVISAVDRGTSSSSQTLPQSEQVSQPAASSNFSSLWGD"
     gene            complement(30891..31064)
                     /locus_tag="RH033_000775"
     CDS             complement(30891..31064)
                     /locus_tag="RH033_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2990007.1"
                     /translation="MAISVKPVLISEKQMEAIKKIQEEQRKKSEVGVAPTIHEIARGL
                     MDKALAYTLTGRG"
     gene            complement(31245..31703)
                     /locus_tag="RH033_000776"
     CDS             complement(31245..31703)
                     /locus_tag="RH033_000776"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2990008.1"
                     /translation="MMKIIAKAFHHIFGKVSDLPSVIPMNNRRETLLDVVVFPYIDLS
                     EPMKATLFRKYLGDFKRRFTGVNGMVDICAIRDIVELAGINLWGEAAAAYKWLERLHC
                     VSFSDMSADIALMVPHKINMVFAGGNYTYPWNKEQRENRKAHAMKSNATP"
     gene            complement(31937..34693)
                     /locus_tag="RH033_000777"
     CDS             complement(31937..34693)
                     /locus_tag="RH033_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HDV2990009.1"
                     /translation="MRNIDLIRQVISASENNWPHVLGCLNINVPDSPRRHAPCPACGG
                     KDRFRFDDNGRGSFICNQCGAGDGLDLIKRVNNCDTTEAALLAADVLGIDYRTTETPE
                     ATSQKREQLETERQRREQERLKRAEKDEQQRRDTFSRQFDDMRRKAVNGKSDYLVAKG
                     VGDFTFPVLPDGSLLLALVDKSGAVTAAQTITSHGEKRLLTGSAKRGAYHAINAPETT
                     QSILIAEGLATALSVHLIRPEALTVAAIDAGNLLYVAQALRDKFPSAQIIIAADNDHS
                     EGRQNTGRIAAEKAALSVSGWVALPPTDHKADWNDYHQKHGIKCATEAFNKSMYQPQG
                     NGVKQEPQTIEGSDFKVMDTDPLKPRIESREDGIYWVSPRADSQSGEIINNESWLCSP
                     LSVIGTGRDDKDQYLILRWLSFGSETPTTAAIPLADIGEREGWRTLKAGGVNVTTKSS
                     LRAILADWLQRSGSRELWRVAHATGWQCGAYIMPDGEIIGTPENPVLFSGRSSAAAGY
                     TVSGSAKSWRDNVARLAFGNYSMMTGIGAALAAPLIGLVGADGFGIHFYEQSSAGKTT
                     TANVASSLYGNPDLLRLTWYGTALGLANEAAAHNDGLMPLDEVGQGADPVSVSQSAYA
                     LFNGVGKLQGAKDGGNRDLKRWRTVAISTGEMDLETFIATSGRKTKAGQLVRLLNIPL
                     SKAVRFHDYQNGKQHADALKDAYQNHHGAAGREWIKWLADHQQQAIKTVRDCESRWRS
                     LIPSDYGEQVHRVAARFAILEAALLLGEVVTGWDAQTCRDAIQHSYNAWLREFGTGNK
                     EHQQIIEQTEAFLNAYGLSRFAPFPYNPADLPIKDLAGYRQRGEHDESPMIFYTFPAT
                     FEKEIACGFNAKQFAEVLKKAGMLTPPNSGRGYQRKSPRIQGRQINVYVLNYQPGDYN
                     SSEE"
     gene            complement(34706..35308)
                     /locus_tag="RH033_000778"
     CDS             complement(34706..35308)
                     /locus_tag="RH033_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2990010.1"
                     /translation="MNNFLTFHAEATPDGVNIMHRSNDGMTERVETVSYIDAVNRLDA
                     GDYDDKPDEGMFIHLAIASGGNQGYFDYTSQHHVIMWRWLIATAFINEMRKENGTVSI
                     IDDSGNHSVVSVYSNGIVAMPLYPVAERLAMANNIEGAMIEKYGVDVGTKNAIIFYSN
                     MFDVEQGTLTSFGREVLADLHNSFIAELNENGIPEAPVTH"
     gene            complement(35301..35522)
                     /locus_tag="RH033_000779"
     CDS             complement(35301..35522)
                     /locus_tag="RH033_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2990011.1"
                     /translation="MTNIQLIEAQCRIEQVQTVLGFWLEGASPSNRDKLMIGAVMSLL
                     NGVPEAIQEADELLGKYELQNHSGEAKHE"
     gene            complement(35519..35782)
                     /locus_tag="RH033_000780"
     CDS             complement(35519..35782)
                     /locus_tag="RH033_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinic acetylcholine receptor subunit beta"
                     /protein_id="HDV2990012.1"
                     /translation="MNINLIYRHPCELEIESLLGREEPYPDTFTPADCATERLTRART
                     GLVHVMNEIVPSVGGEQATVINSWLQKVTSLIDIGLIDVESAK"
     gene            complement(35779..35973)
                     /locus_tag="RH033_000781"
     CDS             complement(35779..35973)
                     /locus_tag="RH033_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2990013.1"
                     /translation="MNNSINTPRLTSALQLIEQAAAVLVAVSLSAEEMDAADVVDAIK
                     ACSSLVNDARAELVILGGEK"
     gene            complement(35966..37033)
                     /locus_tag="RH033_000782"
     CDS             complement(35966..37033)
                     /locus_tag="RH033_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HDV2990014.1"
                     /translation="MIPVQKKIFSLAGISPKSSNMAAKSGINTADTSKVYHLLVVGAD
                     ALTMSEITVDDVSVEKVSSCAREFLVVDDLLCSRSDSTKTFIRARDVNEMSAMYCAFG
                     SSNSEFSESIKKSLPLCGNTVYGYKAPHKTGAGIGVLKILKATYDAPSVFFYVVGLTH
                     PFFGRWCIIQRLCQSMVAQAGASSEAPVSFRAGYANPVWATTSEIGVSGGSVTCYLKE
                     AATCWLLPLPKNRNLSGLSPQFAAIARQLPPKFIILLPSLNAMLAVLWCAITSAFLLV
                     VSAWRWHMIKTYDVHMDPLERTSQIITLTEVINDILVSNSPSRDERLKALLAILDLAV
                     RDVHFLLEGGEMPGKTGATNE"
     gene            complement(37030..37209)
                     /locus_tag="RH033_000783"
     CDS             complement(37030..37209)
                     /locus_tag="RH033_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HDV2990015.1"
                     /translation="MSKPTGTPQPQKRYKDAHGALVTVKSVSHNRVTFYRDGYQSPCV
                     QPLARFMKELAEVNQ"
     gene            complement(37202..38035)
                     /locus_tag="RH033_000784"
     CDS             complement(37202..38035)
                     /locus_tag="RH033_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HDV2990016.1"
                     /translation="MNIEKSRLISEDAPHLNASLGTINGNEFAAIVPVIPGHIGGRET
                     NIVSAKALHKALGVGKDFSTWITDRISEYDFTIGHDYSVHKTISPNLGKSPNGAAYSK
                     IKQSGRPGKDYLLSVGMAKELAMIERNDQGRAIRRYFIQCEEELQRSVPEIAARYRRQ
                     LKARISAANNFKPMCDALNMARAEQGKTTQQHHYTNESNMISRIVLGGLTAKQWARIN
                     GYSGEPRDHMNAEQLEHLSYLESTNITLIDMGMEYEQRKGELTRLSQRWLAKRLEAVN
                     V"
     gene            complement(38048..38479)
                     /locus_tag="RH033_000785"
     CDS             complement(38048..38479)
                     /locus_tag="RH033_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2990017.1"
                     /translation="MEKKNRPLQAANSDIRVSDITPLTKSLQAPKRTPKKHRARVYML
                     RTGIEGWTENDILRYCRLSSGRNYATELERQLGITLERIDEKNPDGIGTHLRYRFSCR
                     GDVLKVITHVNHLANINDHNGLSQQEIADILKLYPDAFNAA"
     gene            complement(38479..38682)
                     /locus_tag="RH033_000786"
     CDS             complement(38479..38682)
                     /locus_tag="RH033_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HDV2990018.1"
                     /translation="MNTDTIPTKGYIRRFRLAELLGVSVSTIDRKVRNGSLPRPVKLG
                     EKITAFDAVEIHNWLAERRGKVA"
     gene            complement(38797..39762)
                     /locus_tag="RH033_000787"
     CDS             complement(38797..39762)
                     /locus_tag="RH033_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2990019.1"
                     /translation="MNDSHETTAVHRNLWQESGIPPLDYCTIERASKLLNCEIDDIWH
                     WKDRLHIDFYVYADFLDFIESDATIHFKKDANFITDLLLMYPEEVRFTFPFLITSASN
                     EVYIPASESGAYELFYAHKPLTVEDSDNKNVFEQIGKNSFIVRKSIACGLLRVRGRTT
                     RHYHSQECGEPYVRLEATFDCDLYKFKMQSSQIHLSSLSDKNIYITKADLEYLHDAKV
                     NGSFDAAKSKKRYEMAVEQELIPEIKNSNHNAERHAENREKLFKSAIYLLSKYPDECR
                     GKKKEISPEKWRDCIVAHKNEIPPLAITNEDVILRHLRAAANGKG"
     gene            complement(39858..41117)
                     /locus_tag="RH033_000788"
     CDS             complement(39858..41117)
                     /locus_tag="RH033_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDV2990020.1"
                     /translation="MPKLTDMQIRAWIKAGDRFDGKADGNGLYICYPKNYTVPFWRFR
                     YKLAGKQRAMVIGSYSELSLSKARETAKELSARVALGYDVAAEKQERKAEALAKMEAE
                     KNAMRVSDLASEYFERQILPRWKHPDILRRRIDKDINPCIGHMKVEDVKPRHIDDMLK
                     GIIDRGAPTIATDVLRWTRRIFDYGIKRHALEINPCSAFEVSDAGGKEVSRDRWLTRD
                     ELIRLFEAMRTAKGFSRQNELTFKLLLALCVRKMELCAARWEEFDLDGAVWHLPEERS
                     KNGDPIDIPLPSPAVEWLRELHTFSCNSAWVLPARKMQNRMIPHIQESTLPVALAKVR
                     AEMPDVPNFTIHDFRRTARTHLAALGVDPVVAERCLNHRIKGVEGIYNRHQYFDERKA
                     ALAQWADMLVALESGKDYNVTPLRRAN"
     gene            complement(41306..42169)
                     /locus_tag="RH033_000789"
     CDS             complement(41306..42169)
                     /locus_tag="RH033_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDV2990021.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIIARETEGQALKVLIEQRL
                     EGVTAEVAKVRAHMPEILQWQRERLIAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            42296..43012
                     /gene="rph"
                     /locus_tag="RH033_000790"
     CDS             42296..43012
                     /gene="rph"
                     /locus_tag="RH033_000790"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDV2990022.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            43078..43719
                     /gene="pyrE"
                     /locus_tag="RH033_000791"
     CDS             43078..43719
                     /gene="pyrE"
                     /locus_tag="RH033_000791"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDV2990023.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(43756..44352)
                     /gene="slmA"
                     /locus_tag="RH033_000792"
     CDS             complement(43756..44352)
                     /gene="slmA"
                     /locus_tag="RH033_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HDV2990024.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(44459..44917)
                     /gene="dut"
                     /locus_tag="RH033_000793"
     CDS             complement(44459..44917)
                     /gene="dut"
                     /locus_tag="RH033_000793"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDV2990025.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(44895..46115)
                     /gene="coaBC"
                     /locus_tag="RH033_000794"
     CDS             complement(44895..46115)
                     /gene="coaBC"
                     /locus_tag="RH033_000794"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDV2990026.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            46287..46955
                     /gene="radC"
                     /locus_tag="RH033_000795"
     CDS             46287..46955
                     /gene="radC"
                     /locus_tag="RH033_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDV2990027.1"
                     /translation="MKNNAQLLMPREKMLRFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            47172..47408
                     /gene="rpmB"
                     /locus_tag="RH033_000796"
     CDS             47172..47408
                     /gene="rpmB"
                     /locus_tag="RH033_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDV2990028.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            47429..47596
                     /gene="rpmG"
                     /locus_tag="RH033_000797"
     CDS             47429..47596
                     /gene="rpmG"
                     /locus_tag="RH033_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDV2990029.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            47694..48503
                     /gene="mutM"
                     /locus_tag="RH033_000798"
     CDS             47694..48503
                     /gene="mutM"
                     /locus_tag="RH033_000798"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HDV2990030.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGESCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(48542..49021)
                     /gene="coaD"
                     /locus_tag="RH033_000799"
     CDS             complement(48542..49021)
                     /gene="coaD"
                     /locus_tag="RH033_000799"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDV2990031.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(49029..50306)
                     /gene="waaA"
                     /locus_tag="RH033_000800"
     CDS             complement(49029..50306)
                     /gene="waaA"
                     /locus_tag="RH033_000800"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HDV2990032.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATSLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            50719..51777
                     /gene="rfaQ"
                     /locus_tag="RH033_000801"
     CDS             50719..51777
                     /gene="rfaQ"
                     /locus_tag="RH033_000801"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HDV2990033.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRNSFTHLVPLQGGNVVESNLSVLTPLGLESLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            51774..52898
                     /locus_tag="RH033_000802"
     CDS             51774..52898
                     /locus_tag="RH033_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDV2990034.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQFSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            52891..53688
                     /gene="rfaP"
                     /locus_tag="RH033_000803"
     CDS             52891..53688
                     /gene="rfaP"
                     /locus_tag="RH033_000803"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HDV2990035.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKKEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVASLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            53704..>54406
                     /locus_tag="RH033_000804"
     CDS             53704..>54406
                     /locus_tag="RH033_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,3-galactosyltransferase"
                     /protein_id="HDV2990036.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAIVSRLTY
                     MDQDILNLILLGKVKL"
BASE COUNT        12974 a        13249 c        13834 g        14349 t
ORIGIN      
        1 aaattaataa ccagatatcg gaatattcgc tctcgcaggg atggtttaca aaatgcgtta
       61 tcggtctcag cacccctata gcatcaagga aaagcagatg aagagtgaag tgttgtccgt
      121 taaagagaaa attggttatg gcatgggaga cgccgccagc cacattattt tcgataacgt
      181 aatgttatat atgatgttct tttataccga tatttttggc attcctgccg gatttgtcgg
      241 aaccatgttt ttggtcgctc gtgcactgga tgcgatttcc gatccttgca tggggttgtt
      301 ggccgatcga acgcgctctc gctggggtaa atttcgtccg tgggtactgt ttggcgcact
      361 gccatttggg atcgtctgtg tactggccta tagcacgcca gatctcagta tgaacggaaa
      421 aatgatttat gcagcaatta cttacaccct acttacctta ctttatacca tcgttaatat
      481 cccttactgc gcattgggtg gtgtaatcac caatgacccg actcagcgta tctcgctgca
      541 atcctggcgt tttgtgctgg cgaccgcggg aggcatgctc tctactgtgc tgatgatgcc
      601 gctggttaat ttaattggtg gtgataataa accactcggt ttccagggcg gtatcgcggt
      661 cctttccgtg gtggcattca tgatgctggc attctgtttc ttcaccacta aagaacgcgt
      721 tgaagcacca cctacaacga cgtctatgcg ggaagattta cgtgatatct ggcaaaacga
      781 ccagtggcgt attgtcggtt tcctgaccat tttcaatatt ctggcggtat gcgtacgcgg
      841 cggggcgatg atgtattacg tcacatggat tttgggtacg ccggaagtgt ttgtcgcttt
      901 tcttaccact tattgcgtgg gtaacctgat tggttccgca ctggcgaaac cgctgaccga
      961 ctggaaatgt aaagtcacta tcttctggtg gacgaacgcc ctgctggcag tgattagcct
     1021 ggcgatgttc tttgttccta tgcaggccag catcaccatg tttgtcttca tcttcgtgat
     1081 tggtgtgttg catcaactgg tgacacctat ccagtgggta atgatgtccg ataccgtcga
     1141 ttacggcgag tggtgcaatg gtaaacgcct gaccgggatc agttttgctg gcacgctgtt
     1201 tgtgctcaag ctggggttgg ccttcggcgg cgctcttatc ggctggatgc tggcttatgg
     1261 cggatatgat gcggcagaaa aagcgcagaa cagcgccacg attagcatca ttatcgcgct
     1321 attcacgatt gttccggcga tctgttactt gctgagcgcg attatcgcta aacgctacta
     1381 ctcactcacg acgcacaatc tgaaaaccgt tatggaacag ctggctcagg gcaaacgccg
     1441 ttgccagcaa caattcacct ctcaagaagt gcagaactaa ggaacggcaa tgaaaattag
     1501 cgacggaaac tggttgattc aacctggcct caatttgatt cacccgcttc aggtgttcga
     1561 ggttgaacag cagggtaatg aaatggtggt ctatgctgcc ccccgtgatg tgcgtgaacg
     1621 tacctggcag cttgatacac ctttatttac gctgcgcttt ttctccccac aggaaggtat
     1681 tgttggtgta cggattgagc attttcaggg ggcgctgaat aacggtcctc attatccgct
     1741 caatattttg caggatgtga aggtcacaat cgaaaacaca gaacgttatg ctgagtttaa
     1801 aagtggcaac ttaagcgcgc gtgtcagcaa aggtgagttc tggtcactgg attttctgca
     1861 caacggcgaa cgtattaccg gtagtcaggt gaaaaataat ggctacgtgc aggacacgaa
     1921 tagtcaacga aattatatgt ttgagcggct ggatcttggc gttggcgaaa cagtttacgg
     1981 tctgggagag cgctttactg cactggtgcg caatggccag acggtagaga cctggaaccg
     2041 ggacggcggc acaagtactg aacaggcgta taaaaatatt ccgttctata tgactaaccg
     2101 tggttatggg gtactggtca atcatcctca atgcgtctct tttgaagtgg gatcggagaa
     2161 agtctccaaa gtgcagttca gcgttgagag tgaatatctc gaatactttg ttatcgacgg
     2221 cccgacgccg aaagcggtac ttgatcgtta tacccgtttt actggtcgtc cggcgctgcc
     2281 gcccgcgtgg tccttcggcc tgtggctaac cacttcattt accaccaact acgacgaagc
     2341 gacggtaaac agctttatcg atggtatggc ggaacgcaat ctgccactgc atgttttcca
     2401 ctttgactgt ttctggatga aagccttcca gtggtgcgat tttgagtggg acccgctgac
     2461 tttcccggac ccggaaggga tgatccgccg cctgaaagcg aaagggctaa aaatctgcgt
     2521 ctggattaac ccctatatcg ggcaaaaatc ccctgtcttt aaagagttac aagagaaagg
     2581 ctatttactc aaacgcccgg acggttcgtt atggcagtgg gataaatggc agccaggtct
     2641 ggcgatttat gactttacca atccggatgc ctgcaaatgg tacgccgaca aactgaaagg
     2701 tctggtcgcg atgggcgttg attgctttaa gaccgacttt ggcgaacgta ttccaaccga
     2761 tgttcaatgg tttgatggtt ccgagccgca gaaaatgcat aaccattatg cgtacatcta
     2821 caacgaactg gtgtggaacg tgctcaagga caccgttggt gaggaagaag ccgtcttgtt
     2881 tgcccgctcg gcctccgtcg gtgcgcagaa attcccggta cactggggtg gtgactgtta
     2941 cgctaactac gaatcaatgg cggaaagcct gcgcggtggt ttgtctattg gcctttcagg
     3001 ttttggcttc tggagccacg atatcggcgg ctttgaaaat accgctccgg cgcacgttta
     3061 caaacgctgg tgcgcgtttg gtttgctctc cagccatagc cgtttacatg gcagcaaatc
     3121 ttatcgtgtg ccgtgggcct acgatgatga gtcctgtgat gtggtgcgct tcttcacgca
     3181 actgaaatgc cgcatgatgc cgtatctgta tcgtgaagct gcacgtgcaa acgcgcgggg
     3241 tacgccgatg atgcgggcca tgatgatgga gttcccggat gatccggctt gtgattacct
     3301 tgaccgtcaa tacatgttag gcgacaacgt gatggttgcg cctgtgttca ctgaagcggg
     3361 cgatgtgcag ttctacctgc cggaaggtcg ctggacacac ctgtggcaca acgatgaact
     3421 cgacggtagt cgctggcata aacagcagca cagcttcctg agtctgcccg tttatgtgcg
     3481 tgatagcacc ctactggcgc tgggcaacaa cgatcaacgt cccgattacg cgtggcacga
     3541 aggtacggca ttccacctct ttaatctgca agacgggcat gaagccgtct gtgaagtgcc
     3601 cgatgctgac ggttcggtga tctttacttt aaaagcagca cgtactggca acacgattac
     3661 tgtgactggt acgggcgagg cgaagaactg gacactgtgc ttgcgcaata ttgtgaaagt
     3721 aaatggtctg caaggcggtt cgcaggctga aagtgagcag ggactggtgg tgactcctca
     3781 agggaatgcg ctgacaatta cgttgtaatc tattgaagaa tgccggatac gaatggtttt
     3841 gtgtcgtatc cggtaaataa tctcacatct ggtaataaat atttcttcca tcgtatttgc
     3901 gagcatgatc acgattattc atcacgaaaa tgtgatctgt gctgaaaaat atcccttaac
     3961 cgccttgctt tgttttatca ctgtccggat tacggacaga ttttagtatt gttgtgaaat
     4021 tattctgctt tatatttacc ctcagagatg gcaactgaag ctcttccata tggggcttct
     4081 taatctgtga taagggtaaa aaatgcaaat gattacctcg cgacaaaaca gattattacg
     4141 attcctttta cctcgaaggg aatatacgac tattgttacg attgccggct atttaaatgt
     4201 ttcggaaaaa accattcaac gtgatttacg tttacttgag caatggctgg gacaatggag
     4261 aataaatgtt gagaagcgtg ctggcgcggg tgtgatgtta agcgcggaga atattgctga
     4321 tttgctgcat cttgatcatt tgttggtggc agaatgtgaa gagattgatg gtgtaatgaa
     4381 taatgccagg cgcgttaaaa tagcgtcgca gttattaagt gaaacaccga atgaaacgtc
     4441 gatcagtaaa ttgtcagaac gctactttat cagcggagcc tctattgtta atgacctgag
     4501 agtaattgag tcctggcttg cgccgttggg gttatcattg atccgcagcc caagtggtac
     4561 gcatattgaa ggtagtgaag ggcaagtccg acaggcaatg gcattactga ttaacggcat
     4621 tattaaccat aatgagccgc aaggtgtcgt gtattcacgt ctggatcccg gaagttataa
     4681 agcattagtc cattattttg gagaagaaga ggtgttattt gtccagtcat tgttactgga
     4741 tatggaaaat gaattaagtt ggtctttggg agaaccttat tacgttaaca tttttactca
     4801 catccttatt atgatgtatc gcaacacgca cgggaatgcg ttatcaagag aagaagatca
     4861 aaccaggcaa tatgatgaaa atatctttaa tgttgccagt cagatgattc ataagataga
     4921 acaacgaatt gcacatacat tgcccgatga tgaagtctgg tttatttatc aatatatcat
     4981 ttcatcaggt gtggcgattg atggacaaaa agatgttagc attatttcac atatgcaggc
     5041 cagcaatgaa gcgcgtctga ttacctggcg tttaattacg gtattcagtg acatcgtgga
     5101 ctgcgatttt agtgaagaca gcgcattata tgatggctta ttggtgcata ttaaaccgct
     5161 gattaaccga ctaaattatc gtattcatat ccgtaatcca ttgttggaag atattaaagc
     5221 agaactagcg gatgtctggc ggttgacgca atatgtggtg aatcaggtat ttaaaacctg
     5281 gggtgagaat gcagtgagcg aggatgaagt aggttacctg accgttcatt ttcaggctgc
     5341 gatggagcgg caaattgccc gtaaacgcgt attactggtc tgtgcaaccg gaatcggaac
     5401 ttcgcatcta ctgaaaagtc gtattctgcg agcatttcct gaatggacga ttgttgatgt
     5461 tatttcagca gcgaatttat cacaggtttt gcctgacaat atcgaactga ttatttcgac
     5521 cattaatttg cctacagtca ctatgccggt cgcttatgtt accgcttttt ttaatgatgc
     5581 cgatattaag cgggtcactg aaatggtgat tacggaaaaa ttacatcatg cgacatctcg
     5641 ggtcgttgaa atttaatgtc agaattttat gaggtatgac gaatggacat aacaaaaatc
     5701 ctgaatacga atcgcgttat tttagatatg caagcgacga ataaagagga ggcgattgaa
     5761 gaattaacta atcttctcaa aaaagatggt gcgatcgatt gtcgggaaac ctttattaaa
     5821 gatgtctggc agcgcgaagc ggaaggctcg actggttttg aaaatcatat tgcgatcccg
     5881 catggtaaat cttcagctgt gattaatact acgctggcaa ttggccgtac ccggcaggat
     5941 attccctggg aaacgctgga tggcagtagc gtgcgctgta ttattttatt tgcggtacgt
     6001 cttgaagatc aaaataccac ccatatccgt ttactttccc aggtcgcggg cgcgctggcg
     6061 gatgatgaca tcatagaaca attgttagtt gagacgagtc cgcaaaaaat cattgatctc
     6121 tttagtcagt atgcacaatc cgatgtctgc taaataacag ggagtatgtc atgaatattg
     6181 tctgtgttgc cgcctgtacg gcgggtattg ctcataccta tattgctcgt gaaaagttaa
     6241 tcaaaggagc aaaagctctg ggtcatacca ttaaagttga aacccaggga accattggta
     6301 cagaaaatga attacaggcg gcagatatct ctgctgctga cgtcgtgatt cttgctgtag
     6361 atgtgaaaat aaaaggagaa gaacgcttca ccaataaacg tattgtgcga gtaaagacag
     6421 agatcgtcat taaatcgccc gttcaatttc ttgaaaaggt cgaaaaatca ctgggaaaca
     6481 aatgatcctt atactggagg gatcttaata tgaaagacaa taaaattccg gtttcacagg
     6541 aaattaaaaa acatttatta accggcatct cgtggatgat accccttatt gtggccgccg
     6601 ggatatgtat tgcgttgggg caagttcttg gcggtacaga tgttggtgaa aaaacaggaa
     6661 caatcccgtg gatgcttaat cagattggcg gctggggaat ggggctgatt gtgccgctga
     6721 ttagcgcggc aattgcctat tctattgccg atcgtcccgg atttgcccca ggcctgattg
     6781 tcggctttct ctgcgggcaa atccataccg gatttattgg cggtatgttg ggtggtttcc
     6841 tggtgggcta caccattttg ctacttaaac gctatatccg cctgccgcag tcgatgcaag
     6901 gactcatgcc aatcatggtg ttgccggtat taagcaccat tatcggtggg ctgttaatga
     6961 tgacgctgat tggtaaaccc atcgcctggt tacaagaagc actgattcat ttgctcgagt
     7021 cgatgcaggg tggatcgcgc ttcctgatgg gggccatact tggcgcgatg gcaaccttcg
     7081 atttcggtgg tccggtcaac aaaaccatgt cgctgttttc cgatggcttg ctggtcagtg
     7141 gcgtctacgg accagaagcc gtgaaatttg tcggctcaat catccctccg tttggcatca
     7201 cactttcgtt cttactgaca cgccataaat atacccgtgc ggagcgcgag gcgctgaaag
     7261 ccgcgtttcc gatggggatc tgcatgatca ctgaaggagt cattccgatc gccgcgcgtg
     7321 acctgctgcg agtagtggga tcttgtgtgg tggcatcggc ggtcgcgggc ggcttaatca
     7381 tggtgtgggg cgtggagagt ccggttccgc atggcggcat gtttgttgtg cctctgttca
     7441 cccatcctct gctgttttgc ctggcgctgg gtataggcac ggtgatttgt ggcgtgatgc
     7501 tttcactctg gaagaaaccg gtaacggaac gcgacgaaga gtttgacgaa ctgagcgatc
     7561 aaaagctgaa agatgaagag atcactttca ccctcgaata accggaggcc ctatgtttgc
     7621 cgatatgaaa agtatggtga tgaaagcctg gcatgaacat tacgccttgc tggcgatcaa
     7681 ctgtatgaat ctggaaagtg ctcacgcagc tatccgcgtg gcggagaaga atcgcgctcc
     7741 gatcattctc aatctctatc aggggcatct ggcgcatttt cctgcacccg tcgcggcggc
     7801 agtggttaaa acgctcgcgg aatctgcctc agtaccggtc actctggcgc ttgatcatgg
     7861 caaaaatccg gactgtattc gtcaggcgtt tcgtgctggt ttcagcggat taatgattga
     7921 tgcttctgct tttcccctgg aggaaaacgt gcgccagacc agggcggtgg tggaactcgc
     7981 cgccagcgcg cagctctgcg tggaaggcga actgggccat ctggcagatg cgccgcgtta
     8041 cgaccaggcc gccaatgcag acctgatgac acaacccact gatgtggaaa cgtttatcgc
     8101 gcagacaggc attgatctac tggccgtctc ggtaggcacg gcgcacggca tgtacgcgcc
     8161 tggtgttgta cctgctattg attttcagcg tctggcggag atttcgcgct gctcgagcgt
     8221 gcctctggca ttacatggcg gtagcggtac gccgttcgat cagctccagc tctgtacgac
     8281 gctgggggta gcaaaaatca acgtgggggc ggccattttt gaacggggga aatccgcgct
     8341 cctgcatacc ctctgtcacg atatatccat tgaactggtg gacgcgctga aagctatgga
     8401 actggccttc gaagaagcga ttacaccgta tttacaggcg agcggctcta tcggcaaagc
     8461 ctgattcatt ttctgtcatg gcctttatgc cagtgcaata acgcaccggc agggcggact
     8521 acacgctgaa actggagatt taacatgtta gtttcgatga aagaattact gcaacctacc
     8581 cgccagcacg gctttgccat tggcgcattt aatgtggcgg ataactgttt ccttcgcgca
     8641 gtcgtggatg aagcagaagc gacaaatact ccggcgatta ttgccatcca tcccagcgaa
     8701 catgattttg tgggcgatgc ctttttcgct cacgttcgcg aaattaccca gcgtagtccg
     8761 gtcccgtttg tactgcacct cgatcatggt gcctctgtag agcatgtttt gcgtgcgatt
     8821 cgctgcggat ttacctcagt aatgatcgac ggttcgctgc tgccgtatga agaaaacgtg
     8881 gcgctgaccg cagaagtggt tcgcctcgct cacgcggttg gcgtgtcggt tgagggtgaa
     8941 ctgggaacca tcggacaaac aggaacgtcg gtagaaggtg gtgtttcaca agtgacctac
     9001 acagatccgg cgcaggccgc cgattttatt gctcgtacag gtgccgacac actggctgtg
     9061 gcgattggta cagcgcatgg catctatcca aaagggatgc agccgaagct gcaaatggac
     9121 attttgcgcg acattgccgg acgtattgat attccgctgg tgctacacgg tggttcggca
     9181 aacccggacg cggagattgc tgaatcagtt acgctcggcg tcggcaaaat caatatctcc
     9241 agcgatatga aatacgccta tttccagaaa gtgcgtgaaa ttctcgctaa agagagctgg
     9301 tgggacccga acgtcatcta tccggatgcg attaacgcgg cgcgggaagt cattcgccac
     9361 aaaatgaaac tgttcggttc gctcggtaaa gcatcactgt actaagccag aagggcgggg
     9421 tgacccgcct tttacgcaac aaggaacgag atatgcacta tctggggttg gatattggtg
     9481 ggaccaaaat cgccgccgtc gtcatggatg cgcatggctg ggagattcgc cgttaccgct
     9541 gcccgacgca aaagtcgaca tatcaacaat ttgtctcatg cgttgtggcg cttatcgagc
     9601 agattaggcg ggacgttcaa cgaccgatgc tgacagggat cgccttaccc ggcagtatct
     9661 cgccactcac tggcctgatt aaaaacgcga atattcaggt gattaacggt catgcgttac
     9721 aggctgattt gcagcaattg cttgggcaac cggtggtgat agccaatgat ggtaactgtt
     9781 tcgcgctatc agaagcttgc gacggtgccg ggcaagatta tgacgtggta tttggtatta
     9841 cgcttggtac gggctgcggc ggtggcattg ccatcaagca acgaccgttt ataggggcct
     9901 ggggaaatgc tgccgaatgc ggtcatatca cgttgccagg ctatacggag caggaagatg
     9961 gtccatcagt cagttgctat tgcggcaaac acaactgcgt ggagtcgttt gtttccggca
    10021 gcggttttag tgaacgctat caacagatga ctggtaactt gctcactcct gcggcgattg
    10081 tcaccctggc acaacgtggt gatgcttgtg ccatgcagca ggtggcacgt tttcgccaac
    10141 agcttgcccg cacgctggca accatcgtta atgttgttga ccctggcgtg attgtgatcg
    10201 gcggcgggct ttcgaatgtg gaactgctta tcaccgatct gaacgcagaa gtcgctccac
    10261 tggttttcac cgatcaattc accactccca ttgtaaaagc acagcacggc gacagtagcg
    10321 gaatgcgtgg cgctgcctgg cttgctatgc gcaacggaga agccaatgaa acgttcacaa
    10381 attaattatg ttattgataa agcacatgcc attgcgcaga ccttccgcgt ttgtttgcca
    10441 gagttcgcct attttacggt ggatgactgg ctgcagcggg aacgggataa ctggcaagaa
    10501 gtggtcgact tacagctcgg ttgggatatc accgattttg gtcgtggtga tttcaaccaa
    10561 acgggactaa cactgctaac gatgcgcaac ggcgcgctgg ggtcggcggc atatccaaaa
    10621 ccgtatgctg agaaaatgtt gcagatccaa caagatcagc agactccctg gcattttcac
    10681 acccacaaaa tggaagatat cctcaatcgg ggcggtggag acttatgcat gcagttggcc
    10741 tgggccaacg aagccagtct ctgtgatgat caacgagtca ttaccgtgag tgttgatggt
    10801 cagcggcgta ccatgaaacc tggtgaaacg ctggtgctaa agccggggca gggcatttgc
    10861 ctgccgccgc gtctgtatca tcgcttctgg gcggaaaaag cgtttgttct cggctgggaa
    10921 atttcgatgg taaatgatga ccaacacgat aattattttc ttgagccggg aggacgtttt
    10981 ccggcgatag aagaagatga accggtaaaa tggttattgt gcagtgagta tgctgttttg
    11041 cggtaatagc ggggacgcta acgttgatat ccgttagcgc ccgcgtattt actgcaccgg
    11101 ctccgtcgat gtaacttcgc cactggaaac gacgcctgca ttcatggttt gagtcacttg
    11161 ctgctttgtg gcgttcatgg catgcaattg cccgctaacc gtaggtttca gcgggatatt
    11221 ggcctgaata tcgccgctgg cggtaagctg aatattgccg tcgccagtca acggtaattc
    11281 aggccatccc caatgttgca aaatattcac cggcacaccg cgtccattca ggctgatatg
    11341 ggtctgacgt tgtggcgttt gtgaaacact ggcggtggct tccagaatgc ctttttcagt
    11401 aaatgcactc agttcgctga tattcaccgt gctgctgttg gcagttagcg ccagcgacgg
    11461 acgacggaca tcaacacgat tgaatgtcgc ggcggcggca ttcagattcg ccgagccact
    11521 ccagacgccc catttatgat cggtaaccag cgtcagattg gcaccgtaac catcgagcgc
    11581 ggtgagctgc cacgggaagt cagggtcgat atcaatgatc agattgcggc tggcgctaaa
    11641 tctcttcagt tgcaggctgt ttaaccaacc gggtgtcgtt tccatccaca actgttgcca
    11701 gtttttgggc aaggtatatt ccagtccggc aattgccgca tcatcgagga tcagcgtttt
    11761 cccgtcgcgt tgccagtttc ctgacgttct gaccataccc ccttcccagc ggctggtgaa
    11821 ctggcgcagt gctacgccct gcggggaaaa ttccgcgttt ataatcggat caaataaatg
    11881 cagcgagcca taaataaact cgctggcgtt catcgacagt ttaccttcct gtgtctgcca
    11941 gtcatcttta ctgaaggtca tgttgcgcaa gctgagatcg aggtcggcca ccgcccagtc
    12001 cggaccttgc aaacgagcgt cgatgacttc caggcgacca atctgcaacg acgggacaga
    12061 gcgtaatggc gcaaagaaat cggttagcga tttttcgctt tgtagacgga tatccgccag
    12121 ccgcagattt tccacttgcc agctgccgtc ggcgttacgc tgcgcgtttc cggttaatgt
    12181 cccgcgggcg atgtcggcac ccaggttagt cagcgtaacg cgatcgttat caatactgcc
    12241 ttcaatcagt acattggtgg caggaacatc attgagcgaa agcgatccgg cactaaactg
    12301 aatctgcgcc ttcgtaccca gcactttacc ggcttcaggt gaccacggaa ccacgccgcc
    12361 atttactcgc tgcgcgctta gtttccattc gctattcggg ctattaaacg ccatatcacg
    12421 cagttgcaga cgatcggctt tgaacggtag cggcgcggtc tggtcggtga gattcagcgt
    12481 cccgttttcc agcaggatgg tatcgacatg gcgtggttcg gtcagttgcc gactgcttag
    12541 cgcaatgtcg acactttttg ccaccagggt cgcgggctgg ccatcacgac caaacgtgac
    12601 gttctccagc acgatatgag atggcgcgga aaaacggtga tccatcgctc cgaaggccag
    12661 atgatagtcg ctattctcgg aaacccatgc gctgatatgt tctgctcccc agcgggtttg
    12721 cagaagaaaa tagaggccag cgatcaccac taacagagcg atgagaatgt aaagaagcag
    12781 cttcccaata aatttcatgg tcttccatcc cgtgaaaagc acataaggga gttatgcacg
    12841 atttacgcgc aatcctcaag gcgggaatgg tgaaagaggt gagactgcgg cggtaattat
    12901 cttaccgccg ccagtgaatt actgtttttc tggtgggaaa atcagattca gcacgatggc
    12961 agtaataccg cccgcggcga tcccggagga gagcaggttt ttcagccact caggggcaaa
    13021 ctgcaaaatc agcggctgct gagacacgcc cagaccgacc gccagcgaca gcgcgataat
    13081 cagaatcgcc cgacggttca gcggctcacg agacacaatg cgcacaccgg aggcggcgat
    13141 ggtgccaaac attacaagcg ttgcgccgcc cagaaccggt tctggaatat gttgtacaaa
    13201 accgctcact gccgggaaca gacccagcac gatcaacatc agcgcgacga caaaaccgac
    13261 atagcggctg gcaacaccgg ttaactggat cactccgttg ttctgcccga agcaggagtt
    13321 cgggaaggtg ttaaacaccg ccgaaacaaa cgagttcagg ccgtttgcca gcacgccgcc
    13381 tttcaggcgt ttcatgtaca gcggaccgga cactggctgt tcggaaacgt cagaggtcgc
    13441 cgtgatatcg ccaatggttt ccagcgaagt gatcataaag accagcatca gcggcagcag
    13501 cagactccat tcaatgccaa gaccgtaata gagcggcgtt ggcaccataa tcagttcttg
    13561 tgtcatcggt tcgttgcttt ctggcaacat gcccataaac cacgccagcg catatccggc
    13621 cgccatcgca attaccagtg aggccacgcg taagtaaggg ttacgttgac ggttaagcag
    13681 gataattaag gctaaaacca cgcctgccag cagcagattt ttcggtgcgc cgaaggtgtt
    13741 atcgctcatg gctgcgtaac cgccgccaat ggaggtcagc cccacctgaa ttagcgatag
    13801 gccgataatc atcaccacaa cgccagaaac cagcggcgta ataatgcggc gcgccagatg
    13861 cagaacgcgg gagatcacca tctcggtgca acttgccagc atcaacgtgc caaacaaagc
    13921 cgccatcatg gtaggaacat cagcaccacc ggttttcagc gcggtaccgc ccataatcag
    13981 cggggcaaca aagttgaagc tggtaccctg aatagacaac agcccggagc caaccggacc
    14041 ccaggcctta atttgaataa tcgatgccac accggaggca aacagcgaca tactaataat
    14101 gtgttgcgtg tcttgtgccg gtaaacccag cgcctggcag attaatagcg ctggcgtgat
    14161 caccgcaacg aacatcgcca gcaaatgctg acaggcggca aacagggttt gaggaagcgg
    14221 cggacgatct tcaagacggt aaatcagttc gctgttttga gtctgcgcaa ccggttgcgc
    14281 attttctgac tcgagggtgg aaacagacat cgaaaacaat cccgtggtag caaagcgggc
    14341 attttagctg accgaaagag aaaagcaaac gtttgccaca tcctcatcta aatatttaaa
    14401 tgagataaat gcactttttt atataacttt tggtattttt ctgcttaaaa tccatgccat
    14461 ttgttcatta acagctgaga atttgaccca ggaaagcata actcgatacc cgtcatagtt
    14521 cataagttgc cactgcgcga tgaagtatga cgagtatgaa agagtgatgc ggatacaaag
    14581 gagtaactat gtttcatctc gatactttag caacgcttgt tgccgcaacg ctgacgttgc
    14641 tgctcgggcg taagttggtc cattccgtct cctttttgaa gaaatacacc ataccggaac
    14701 ctgttgcggg tggtttgttg gtggcgctgg cgctactggt actgaaaaaa agcatgggct
    14761 gggaagtcaa ctttgatatg tccctgcgcg atccgttaat gctggcattc ttcgccacca
    14821 ttggcctgaa cgccaacatt gccagtttgc gtgccggtgg gcgtgtggtt ggcatcttct
    14881 tgattgtggt tgttggtctg ttggtgatgc aaaatgccat tggcattggt atggctagcc
    14941 tgttagggct tgatccgctg atggggctgt tggccggttc tattacgctt tccggcggtc
    15001 acggtacggg cgctgcgtgg agtaaattgt tcattgaacg ctatggcttc accaatgcga
    15061 cggaagtggc gatggcctgt gcgacgtttg gtctggtgct gggcggcttg attggcggtc
    15121 cggtagcgcg ctatctggtg aaacactcca ccacgccgaa cggtattccg gatgaccagg
    15181 aagtcccgac ggcgttcgaa aagccggatg tgggccgcat gatcacctcg ctggtgctga
    15241 ttgaaactat cgcgctgatt gctatctgcc tgacggtggg gaaaattgtt gcgcaacttt
    15301 tggctggcac tgcttttgaa ctgccgacct tcgtctgtgt actgtttgtt ggcgtgattc
    15361 tgagcaacgg tctgtcaatg atgggctttt accgcgtctt tgagcgtgcg gtatccgtgc
    15421 tgggtaacgt aagcctgtcg ttgttcctgg cgatggcgct gatggggctg aaactatggg
    15481 agctggcttc gctggcgctg ccgatgctgg cgattctggt ggtacagacc atcttcatgg
    15541 cgttgtatgc catcttcgtt acctggcgca tgatgggcaa aaactacgat gcggcggtgc
    15601 tggctgcggg tcattgtggt tttggcctcg gtgcaacgcc aacggcaatc gccaacatgc
    15661 aggcgatcac tgaacgtttt ggcccgtcgc acatggcatt cctggtagtg ccgatggtcg
    15721 gtgcgttctt tattgatatc gttaacgcgc tggtgattaa gctgtattta atgttgccga
    15781 tttttgccgg ttaaccgatg aagcggcggt agaagtgccg ccgcaacgaa gacaaatgcc
    15841 tgatacgctt cgcttatcaa ggctgcgcat tgattcacaa tttattgagt ttataggttt
    15901 ttgtaggacg aagcacatcc ggcaggaagg tagggtaacc tgaagcggcg gtcttctcac
    15961 cgccgccttt tacgcattcg agtaacgttc cgtctccggc atccagcgtt ctatcagggc
    16021 ttttgcttgt tgtgggtaac gttcgtgaat atgtcgcgcc aggcgctgaa cttccgggat
    16081 catcgcctga tcgcgcagta aatccgccac tttaaactca gcattacccg tctgacgcgt
    16141 acctaacagt tcacccggtc cgcgaatctc taagtctttc tgtgcaatca caaaaccgtc
    16201 gttgctgtcg cgcagtacct gcaggcgaat ttgcgccgtt ttagagagcg gtgttttgta
    16261 gagcaacacg cagtgagaag ccaccgcacc acgacctaca cgcccgcgaa gctggtgcaa
    16321 ctgcgccaga cccagacgct ccgggttttc gataatcatc aggctggcgt taggcacatc
    16381 aacgccgact tcaataacgg tagtggcaac cagcaggtgt aactcgcctt gtttaaatga
    16441 tgccatgacg gcctgcttct cggctggttt catccgtccg tgtaccagac caacattcaa
    16501 ctctggtagc gccagtttca actcttccca ggtagcttcc gccgcctgcg cttccagtaa
    16561 ctctgactct tcaatgagtg tgcacaccca gtacgcctga cgcccttcag tcatacaggc
    16621 gtggcgcacg cggtcgataa tgtcgttacg acgcgtatcc gggattgcca ctgtcgtcac
    16681 tggcgtgcgg cctggcggca gctcatctat caccgaggta tcgagatcgg catacgcggt
    16741 catcgccagc gtgcggggga tcggcgtcgc ggtcatgatc aactgatgcg gatggaagcc
    16801 ctgctgctgg cctttctccc ataacgccag acgctgatgc acgccaaaac gatgctgttc
    16861 gtcgataatc accagcgcca gaccgttaaa ctgcacctgt tcctggaaga tggcgtgtgt
    16921 accgacaatc atctgcacct gaccgctggc gatggcttcc tgctgtgcca gtcgcgcttt
    16981 acctttctgc ttaccggcga gccagcccac ttcgataccg agcggtgcaa accagttgcg
    17041 gaagttattc gcgtgctgtt cggcgagtaa ttcggttggt gccatcaatg ccacctgttt
    17101 gccgtgcgca atcgcacgca gcgcggcgag ggcagcgacc agcgttttac cggaacctac
    17161 atcgccctgt accaggcgca tcatcggcgc atccagcgcc atatcgcgct cgatctccgc
    17221 caccacgcgt gcctgtgcgc ccgttggctt gaacggtaag gctgcgagga gtttattttt
    17281 cagcgcgtca ttggcgctaa gcggctgggc atggaaacgt tgtgcaccag cgcgtaacgc
    17341 caacatgctg aggttgtgcg caagcagttc ttccagaatc agacggcgtt gtgctggatg
    17401 ctgcccggtt tcgagatcgc taagctgtag cgtcggcggc gggcggtgca aagtgcgcaa
    17461 cgcttccggt agcgtcatca ttccttgtga cagttccggc ggcaggagtt cttcaatggc
    17521 gcaggtgtcg agcagatcca gcgcctgatc ggttaattta cgcagcgtgg cctgctttac
    17581 gccttccgtt gttgggtaaa ccggcgttag cgtttcctgc aactctggcg tactgagatc
    17641 gccctgcacg cggtattccg ggtggatcat ctccgcacca tatttaccgc gctttgcttc
    17701 gccataggcc agcacgcggc ggcctgtcgc caggctattt ttcattgccg cgttgaagtt
    17761 gaaaaagcgc atggtgagaa tgccggaacc gtcgctgatc tggcaggtca tcatccgccg
    17821 accgccgaag gagatattgc agttcagcac ttcgccttcc accgtggcat aaacgcccgg
    17881 tagtagttct ccgatggggt agagatgggt gcgatcttcg tagcgcaggg gaaggtgtaa
    17941 gagtagatcc tgcacggtat gcaggttgat tttcgccagc ttgttactaa gcgctgcgcc
    18001 aacgcccgtt agggaactga gcgggacagc atctaacagg cgacctttca tggcacttac
    18061 cctgcagcct gcatggtagc ccaccagtca gcatcggctt caatttcgcc ttgctgatta
    18121 acgtgggggt aaggcaggcc tttgcgtttt gcgactttcg ccagcaccgg atagccgcct
    18181 tcaaacagca ggcgttgttg ctctgcttcc ggcaacatgc tgttttcacg caggtacatg
    18241 cctgcatttt gccgctggcg ctgggcttca taaagaatga gggctgaggc aacggaaaca
    18301 ttcagcgact gcaccatgcc gatcatcggt atgatgatgt cccgatccgc cagggccaac
    18361 gcttcctgcg tgatgcctgt tttctcctgt cccatcaaaa tacaggtcgg gcgagtgtaa
    18421 tcaatttcgc ggaaatcgac agcgttatca gaaagatggg ttgccagaat ctgcatgccc
    18481 tggcctttca gatgagcgac ggcatcgcca atggtgcggt gcgttttcac ctgtacccag
    18541 ctgttactac ccgcagctgc cgaagccatg gtgcgcatcc ggctaccagg ccagacagca
    18601 tgaacttcat gtacaccaac ggcatctgcg gtacgaataa tcgcagaaac gttatgaggt
    18661 ttgtggacct gctccatgca gacggtcaga tcaggctgcc gcctggcgag catttcgcag
    18721 atgcgtgcat aacgtgttgg gttcataaaa cattaatttc ggtttcgggt gactttaatc
    18781 acgtctggca tcacgcggat tttgcgcatg atattcgcca gatgcacacg gtcacgggcg
    18841 gtcagacgaa taaaggcgct gtagacgcga ccatctttct cttccgtatt caaactttga
    18901 atattcgagg tggtggtgtt aattgccgct gtcaggtttg ccagcgcgcc ctgatgattg
    18961 aacatctcca ccttgatttc ggtgatgaac tcctgcgccg tctctttatc ccattccaca
    19021 gccataaact tctctggctc tttctggtag ccacggatgt tacggcagga ttcatggtgg
    19081 attaccagac ctttaccggg gctgacgtgg gcgataatcg ggtcgccagg aatagggcgg
    19141 cagcatttcg caaaggtgat cagcacgcca tcggcacctt taatgggcag atgtccgtga
    19201 ctttgggttg ccggtggaat ggaggcgtcc ccatgttgca gatttttcgc gaccaccacg
    19261 ctcattgcgt taccaaggcc gatttcagcc agcagatcgt caagcgttgc cagcttcatg
    19321 cggtccagct cgcgctgaat attttcctgc gggatttcat tgagcttgcg gctgccaccc
    19381 aacgcatggt tgagcagacg acggcccagg cttacagaat catcacgctt gaggtttttc
    19441 agcaattgac gaattttggc gcgcgctttc gagctaacga caaagttcag ccacgcggca
    19501 ttcgggcgag cgcccggtgc ggtaataatt tcaacggttt gaccgctggt aagcggctgc
    19561 gacagcgggt aaggctggcg gtcgacgcgt gcgcccacgc aggcatggcc gatatcggta
    19621 tgcaccgcat aagcgaagtc gacgggcgtt gcaccggcag gcagttcgac aattcgccct
    19681 tccggtgtga aaacgtaaat ctcatccggg aagagatcgg atttaacgct ctcgataaat
    19741 tcaaacgagc taccggcact ctgttgcaac tctagcaggc tttgcatcca gcgctgggcg
    19801 cggatttgtg cggtggtgct ggtttcgccg tgctctttat aagcccagtg cgcggcaaca
    19861 cccatctctg ccatctgatc catatcttcg gtacggatct ggacctcaac cggcacgccg
    19921 tgcgggccaa tcatcgaggt gtgcaaggac tgatagccgt tcgcttttgg aatggcgatg
    19981 tagtctttca cgcgacccgg acgcggcttg tacaggctat gcatttgccc gagcacgcga
    20041 taacaggtgt cggaatcatt gacgatcacg cggaaagcgt agatgtccat gattgagtga
    20101 aaacgctgct ctttgagcac cattttgcag taaatcgaat aaagatgctt ttcgcgacca
    20161 ctgacgcggc acggaattcc cgcttcctgc aaacgccctt cgatttccga gaggattttt
    20221 tggatcatct ctttacggtt gccgcgcgcg gctttcacca cttctttaat cacgcggtaa
    20281 cggttgggat acagcgcctc aaaacccagc tcttcgagtt cggttttaat gtggtggata
    20341 cctaaacggt gcgccagcgg gctgtaaatt tcgagagttt cacgggcgat gcggcgacgt
    20401 ttgtccgggc gaagtgagcc cagcgtgcgc atgttgtggg tacggtcggc aagtttgatg
    20461 agaatgacgc ggatatcctg caccatcgcc ataatcatct tgcgaaagtt ttcggcctgc
    20521 gcctctttct tatcgcggaa cttgagctta tcaagtttcg acaccccctc taccagctcg
    20581 gcgacgcttt taccaaaaag ctgttccatg tcctggtagg tggcgggagt atcttcaatc
    20641 acgtcatgca gcagcgccgc cataagcgtt tcatagtcga gtttcatctc ggccagaatg
    20701 caggcaaccg ccaccgggtg cgtgatatag ggttcaccgc ttgaacgtgt ttgcccctcg
    20761 tgagcatcac gtgcaacgag atacgcctgc cgcagacgct tgatttggtc ttccggcagg
    20821 taggtttgaa tcagttgatt caggctttca aacagataca agggcgaccc gctttgtgat
    20881 taacgacgac cttcagcaat agcggtaacg gcttgtaatt cagcggcttc ctgctcttgc
    20941 tgttcctggc gttcacgaac gtcgaggatc tggttgttga tcagaccttc ttcgatttcg
    21001 cgcagcgcga ttacagtggt tttatcgttt tcttccggta ccagcggatc ctttccgcct
    21061 acctgcatct gacgagcgcg acgcgcggcg accagtacca ggtcaaaacg gttaccaatt
    21121 ttctctacag cgtcctgaac agttacgcgt gccatactta aaaagctcca caggtgaaga
    21181 aatgactggg catgatactg aaatcaggtt cagtctgcca acaatttgct gattaaagcg
    21241 tcatgacgct gcttttggcg gctcatgcgc agacgttcgg cgcgaataat ggtcttcaaa
    21301 tcggtcaacg cggtatcgaa gtcatcattc acaatcaggt aatcatattc ggcgtaatgg
    21361 ctcatttctg caacagcttg cgccatacgc tttgcaatga cctcttcgct gtcctgaccg
    21421 cgaccgcgta gacggcggtc cagttcaatt ttggacggcg gtaaaataaa gatactccgc
    21481 gcgtgcggca tcttctggcg aatttgctgc gcaccctgcc agtcgatatc gagaaaaaca
    21541 tcgacaccgg tcgccagtac ttgctcaatg gcctcacgcg aagtgccata gtaattacca
    21601 aaaacttctg cgtgttcgag gaacgcatct ctgctaatca tttctttaaa ttcatcatga
    21661 ttaacaaaga aataatgttc accgtggacc tcaccaggac gcggttggcg tgtggtgtgt
    21721 gaaacagaaa cctgggtgtc atacaacggt tgggttttta ataaagcctg aatcaggctg
    21781 gatttacccg cgccactggg ggcagaaaca atataaagcg tgccttgagc catgagtatc
    21841 tttcgtatgt gattagcgaa ataaagccta catacgagct tattatacac ggcgcggcca
    21901 tgtgacgtag cccttgctca cactttttgc gcaaagttgt tgcgttttgc gtagctgctt
    21961 ccgcatttac ctgtcattca ctgcaataaa gacgattctc aggaagcgtc ctcgcagact
    22021 ccgtttttgc cactcggctt tgctttcatc acgttgtata tctgcctgac tatctacagc
    22081 ggaggtgatg atgaaagtat ggatggcgat attaataagt atcttgtgct ggcaatcatc
    22141 ggcgtgggca gtctgtccgg cctggtcgcc agccagagca caggaagaaa tttcccgcct
    22201 gcaacagcaa ataaaacagt gggacgatga ctactggaaa gaagggaaaa gtgaggtgga
    22261 agacggtatt tacgatcagt taagtgcccg tctcacgcag tggcaacgct gttttggtga
    22321 agagacgcat cgcgacgcta tgatgccgcc tttaaacggt gcggttatac atccggttgc
    22381 acatacgggt gtacgcaaaa tggcggataa aatcgcatta agtctgtgga tgcgcgaacg
    22441 tagcgatctt tgggtgcagc caaaagttga tggcgttgcg gtaaccctgg tttatcggga
    22501 cgggaaactg aacaaagcaa tcagtcgcgg caatggcctg aaaggcgagg actggacgca
    22561 gaaagttcgc ttaatttccg ctgtgccgca aaccgttagc gggcctttag ccaacagtac
    22621 gcttcagggg gaaatatttc tcaaacgcga ggggcatatc caaaaacaaa tggggggaat
    22681 aaatgcccgc gcaaaagttg ctggtctgat gatgcgccag gacgatagcg acacgctgaa
    22741 ttctctgggc gtttttgtct gggcatggcc ggatggaccg cagttaatga ccgatcgttt
    22801 aaaagagctg gctaccgctg gttttactct aacgcagacg tatacccgtg cggtgaaaaa
    22861 tgctgatgaa gttgcgcgcg tacgcaatgc gtggtggaaa acgaaattac ccttcgtcac
    22921 cgatggcgtg gttgtgcgag cggcgaaaga acccgaatcc cgccattggc taccaggcca
    22981 ggcagaatgg ctggtggcct ggaaatatca acctgtagct cgggttgccg aagtgaaggc
    23041 gattcagttt gcggtgggta agagcggtaa aatatcggtg gttgcgtcac tcgcacctgt
    23101 catgcttgat gataaaaaag tccagcgggt gaatattggt tccgtcaggc gctggcagga
    23161 gtgggatatt gcgcctggtg atcagatcct cgtcagcctt gccggtcagg gtattcctcg
    23221 cattgatgat gtggtgtggc gcggtgcaga acgtacaaaa ccgacaccgc cagaaaaccg
    23281 ctttaactcg ttgacctgct actttgcttc tgatgtttgt caggaacagt tcatttcacg
    23341 cttagtctgg ctggggtcaa aacaggttct tgggctggat ggcattggtg aggccggctg
    23401 gcgctcgctg catcagactc atcgctttga gcatatcttt tcctggcttt tattaacgcc
    23461 agagcaatta cagaacacgc cggggatcgc gaaaagtaaa agtgcgcagc tatggcatca
    23521 gtttaatctg gctcgtaagc agccttttac tcgctgggtg atggcaatgg gaataccgct
    23581 aacccgggcg gcgcttaatg ccagtgatga acggtcctgg tcgcaacttt tattgagcac
    23641 ggagcagttc tggcagcagc tgccggggac tggatcgggg cgtgtcagac aggttattga
    23701 atggaaggaa aatgcgcaaa tcaagaagct gggcagttgg ctggctgccc agcagatcac
    23761 aggttttgaa ccttagtgtc cttcgtggct gtagtaaaag accggtaatc ccagcttaag
    23821 gcgtagcgcc agtaagcggg caaaaaagcc gaataccagg gtggagataa ttaccacatc
    23881 atggttagaa acatagtgtt gcagcgcgat gtacagcacg gcggaggcaa atgagacacc
    23941 ggcatacaac tctttctgga ataccagcgg gatgcgttta cagaacatat cgcgcaaaac
    24001 gccgccaaat acgccggtcg tcacagctgc gacaacggca atgatcgggc cgtgtcccat
    24061 atccagtgca acctgtgcgc caatgataga aaagaccacc agtccgagcg catcgagcac
    24121 cagaaacact ttgcgcagat aaggcattac aggagcaact atagtggtga gtactgcagc
    24181 ggtagcgacg ataatcacat attccgggtg tttgacccag ccgagcggat agtggcccag
    24241 cagaatatcg cgcactgacc ctccgccaat tgccgtggcg gtcgcaataa taattacgcc
    24301 aaatgtatcc atgcgccgtc gtccggccgc cagcgccccc gtcatggctt cggcagtaat
    24361 gccaaccaaa tacaaaatgt gtaacagcat ggttctctcc ctaaaaatct gtctggaagg
    24421 gtagcgattt gtgcgcattg tcacgattga gattttttaa ggagagaata gattgataat
    24481 ttgtagttat gtgaaattgc tattttctgt agtaagaatg catttttctg attttgtgat
    24541 aagtaaaatg cattagaaaa agtatggagt aaataaatgt gaggacgaaa ctatatgcgt
    24601 agcgccgctg taattaacaa cggcctacgg agatatgtcg cttactgaat ctgtttgtag
    24661 agtgttggct tcaatgccgc attgtggctg gtttctttct aatgcggttt ttgattatgt
    24721 acacccttat gtacacggtt ttttagggtg ctttggtcag aatccattgt ctacatccct
    24781 cacgatcgag gtgtgtaacc atgttcaggt ttgttgtcct gcgcgcgcgt attgctcgtg
    24841 aaattgctgt acccaatagc gatgtccgaa aaaacctccg ttctcacatg aaacagccat
    24901 gtgcaactgg taactgaaca gactggtaga ccgttcgttc agtaaatacc tgatattttc
    24961 gccaacgaaa aaatgcgttg gctggcagag tgcaaatttg cacgatgacc taacccattg
    25021 gtttttccac aatcctcaat ttgaggaatg tacctaaaac catttttata gctccgatcc
    25081 cttcaaagtg aggggtaatt tgccaaaaca gagagagcat tactgtctct ggcaatcttt
    25141 atcgcgataa cgcctgaagt accctctcgc ttcctcatcc ataccaagag ccgaatgagt
    25201 tcgcttaact gcctgtctta aatctccgaa tctgtctgta gttgctggtg ggcgtgccgc
    25261 cttacggata cattcagagc gtctgtttgc tgcatgttca cgttctttat cgccatctgc
    25321 cagcatcatg acttctgccc atcgtgcggc agctctccgg tatagccctt ttgactccag
    25381 ctcttcagct ttcctgtccc ttatcatttc tgcctctcag attatataga taagcaaaaa
    25441 ggtgttggtt cagttggttc agttggttca atttgtaaag agcgttgttt ttattgtgtt
    25501 ttttctaaaa tgttgaacca acatcaactc attttgaacc aacacagccc agctctgaac
    25561 caacaacaca gcgatagatt tgtgattggt ccgttatttt ctggctgata tttgagcgta
    25621 taacagtgtt atacagtggt atactgtatg gatattactc actgatatgg tctttgcaat
    25681 gagcaaatct aatctggtcg cgttccgcat cccatctgaa ttgcaggatg agtttaatcg
    25741 gtctgttctc gcttctggag gggataaaac ctcctggctt gttgatgcga ttcgcatgaa
    25801 gctgggacag ccggaaaagt ctattgattc acgaatgctt gggctggtgg agcgcatgga
    25861 aaaagccgct gcgtcgctta ttgccggaaa gccgaacatc ccaccaaagc catataacga
    25921 aacggcagtt ataaagatca ttgccgatac catccaacag ggatttgata atgggcgtgt
    25981 gatagctgaa cggatcaatg aagcaggcta tcaaacaaaa gctggtaaag cgtgggataa
    26041 agatatttac agcgcgtgga agcgtcaggg aagcaatgct gaaaagttaa aagcggtgat
    26101 tgattgtaag gtcagcgtat aaacatgagt gcccattgga aagctgcttg ggccaatacc
    26161 tttatacacc agcaggtggt gacgatgggt gaggatcagg catgaaaggc agaacgccac
    26221 acattgaaca tcaagccaac cacttctcgg atgttcgcaa tgagatgatt gccattcgcg
    26281 gtgatatgca cggcttggag cgtcgaatta ttgataagat ggatgaaaat agagataagg
    26341 ccagtgaaaa tcaaaagtgg atcatcggcc tgttgatttt atcaattctt atcccgctac
    26401 ttatcgcatt ggtcacaaaa tgaatatcca atgtgtatgc tgtcgatgct acgcgcgcgt
    26461 acttgtctca aaatatcagg caaaggccag attcagaaag tcgtgataac caccagcaca
    26521 acatgagatg tcatgtaacg cgatttatca caccagatac atgtgatggt gtacccgcat
    26581 caaacatcgt catagcgcgt cctgtgcgca tatacgccga taatcaacat atcaccacca
    26641 ctctggctta tgtagagaac agttttatgg tactcccctc catcgtgcgc gtactggtcg
    26701 gacgtagttg caaaattttt tgcaactgat acccctcgcg cgcgtactgg tcacgaaatt
    26761 ggtgttttgc tgtggcagtg atatcagaaa aatcttcctg tttcacataa agcggatatg
    26821 cgcgtactgt aacggcccca tcaacgggga tcggatttga aatcccgacc caagctatgt
    26881 accaaatgcc atgtcttgga tatatgatga ttgccattct aaacaaagct aaagggaggg
    26941 ttggtgtgaa tgctcaggct gtcatcatgg ttttccttgg tctgattgtc gcaaaactat
    27001 atccagactg gtggaaaggg gcattagcaa ctgtcgccat ctatttgctt ttatccgtcg
    27061 ttaagcgatc gttcgttaag accgtaaatc aagcaaaacg tttctcattt aagaatttaa
    27121 taaactatat tactgggctt attttttgcc tttcaatttt gatcgcttca gcttttttgc
    27181 ccgtatcaat agctaatttt tttggcgcac catatgaggt aggttatcta ttctctgttt
    27241 tttgtcttgt tctttccctt ctttacctat ggatgagagc aaagaacaaa aattagccat
    27301 ccatggctaa ccagttattg agcttctttt tgtgatgcct gtgcgactct ggtgagcgat
    27361 tgtgtgaccg tactgatcgc tctgtcatat gctgtgctac cttttggcgt atttgccaga
    27421 cgtaacaagg catttcgtat aacagggctt tcgtatgcct tcgctgctcc gcctacggca
    27481 ccagcagcag gaacaacaaa cgcagccgtt tttagcatcc ccgctaatac tgctgcgttt
    27541 atgatgccac caccaactaa caatccataa agctgctgac catttaagtt ttgaacgttc
    27601 gcttttgccg cgtgtcttgt atcgttgagg tatttaataa cgccgtccag ttcctttcca
    27661 tgtacgcctt taaagaaagt tgctgattgc ttcctgttcc ggtgtaacgc attaataaac
    27721 ttctcaacac tgatatttcc agacggatca gtcgccttat ctacagcatt ctgaactatg
    27781 gctgctcgtg cgttttgcct accattgtca tccagaagcc tgtaaagctg agaacgttct
    27841 gatgggcttt ggctaaaaac gagttttgtt acatcctcag gcgtggcctt gccgctggct
    27901 attgcctttt gaacgcgcgt tcttcccatc atgtcattga atttagacca tgagcgatca
    27961 acccttgcca tattaatggc ttcgtctgct ccaagatttt tggctacggc ttttttcata
    28021 tccgccgtat atgccttgta gataatatcg ctggctttct ggagcgtatc tttatcaaca
    28081 gtgtcagatg acgccataaa tcgctttcga agatcggttc tgttttccct cgccagacgc
    28141 aggtcatttg gtccgctggt gatatcgtct ttaaattgct gcaagacttt taccgcggct
    28201 ctatcctgag atttgcccgg gcggcttaac acggcaatct gattgtcgat agcttttacc
    28261 gtgtttgaga gatcgacagg ggtatccccc atagcgtcaa ttatgcggtt atatcgcttt
    28321 cctgctgcct caatgaattt ttgttgccct ttagtcgcag attcataaag ctgtttatca
    28381 gaaatgccac ccagaccatc gctaaatgtt ctgactaaat tttctcgtgc ttgttgttga
    28441 ttcagccgat tcttaccggt tccaaaaaaa ggaactcttt cgcctaatgt ctgagcctga
    28501 ctctgcataa aggttttatc tttcagcatg tcactggtca ttaacggcag atttctttcc
    28561 gatgcaaagt cgacagcagc ctttgcttca ggcgacatat caccacgaac agccctcgaa
    28621 accgcacttg ctgtattttc cagcccctta ccaataccac caatgccagc agaaattacc
    28681 gtttgtaacg gatcaatatc ttctccacca gcaaggctgg tggctccctg tagtgctaaa
    28741 tctgtagctg ctgattttcc tatggcacca agcaccgttc cagctctgcc cgctggtgta
    28801 aacgccaacg cattagcaag aaacgaagtc aggtcttgcg gtgataaacc cggcttgttc
    28861 agggcataat cacctgatgg cagagaaacg atagtattcc ccttctcatc ctgccttaat
    28921 gtcgccccca ttccctgaag aatcttttcc tgagacgcgt cagagccgaa aagttgagac
    28981 catccagcct ttagggcatc catgcttagt gagttcaatt ctggggcaga ggcgacattt
    29041 ttcagtctct ccatctctgg agtcattcgg ctttcgcctg ttatagcatc gcgcatcgca
    29101 gccccaagac tggctccctg ttctgctgaa cgatcaagcc cctctctttg ctgagtagct
    29161 aactttgcat accctgacgc aagagaatta tcagatgatg tatgttgaat attttgagtt
    29221 gctgattggg atcggtctat ttcattcgtt gatggtagtg gataggcagc ataaaaagcc
    29281 tgcttagcct gctctacact ttctccggct tgtggggcaa cgacttcatt gaagtattgc
    29341 tcctgagcct gcgctttttg ttctggtgct aacgcctgat actgtggaga ggcgataaca
    29401 tctttccatg ctttagccat taatcacccc atagtgaaga aaagttactg ctggctgctg
    29461 gctgtgatac ctgctcagat tgcggcaatg tctgcgacga agaagaagtt cctctgtcaa
    29521 ctgctgaaat aacccgcaaa gcatcataag ctcgaccaga agcagctttc agcgaattga
    29581 actggttttc catcttccgg cgcttggtat caatggtttt tgtggagtcg ccaggctgag
    29641 ggaaatacat ttgataatat tttgacattt cagcaggtgc gatagctgcc cctgtttcct
    29701 gacggagtat tgccattatt gcgtcattag cattaacaac gtattgctgc tctgttgggc
    29761 tgagcgttaa gttagccagc gtcccattac ccaaagcatt attaatgatc gccactcgct
    29821 gtgggctaag ctggtctttc agtgtatcta ttgaatcaag agcatcccta accctgtatg
    29881 caaatccagc agctttttta gccccttctg gcgacttttc catgatcttt tgggctgttg
    29941 gcaaagctat tggacgaaac ccatctccag atattggctg gtttagtttt ccctgctctt
    30001 cacttccgtc tgtgtagtat tttgtgacag tgccatcatc gttttctcta acactcatta
    30061 attttttggc gtttgtatta attccagccg ctgccgcaaa tgccgccgcc ccctctggat
    30121 ccgcctttaa catttgagcg tactgattgt aattctgcat tgctgctgtt ggtgcatatg
    30181 ctgacgttaa cgcatttgct cggctaatat cctgccctct cgcctgaagt gcttcgccag
    30241 cctgattgct gcggattgtt tctgccaact tgccacgctc aatatcccga ccttccattt
    30301 gatctttgat atcgaagtac ttctcatgac cgagagaaaa tagtgccaga ttacctgcga
    30361 agtgctggaa gccatgcggg tcattaacct gcatttcagc aatagtttct ggagggatac
    30421 ctaaacgacg catctcctgc tcattatcca tcatgaacct gccaaatgcg ccctgaccaa
    30481 gcgatgaggc tacctgtgct ttgagcgcca gattgccata atcatccctt tgagcatcat
    30541 cgacataact catcccttta cggacttcct caaactcttc tgggaatgct gtaataagat
    30601 tacgcatctg ctccctgtca ccggacgcat aagcatctgc ataacctttc tggaatgccg
    30661 ctttacgctc ctgctgttgc tgctgcttat aaatatcagc aactccagcc agaccacgaa
    30721 gacctgtcag agcaacgtta tttgcacctg agcgagccag ttcattgttt tcgcggatca
    30781 gaccaagcgt tgcgttaatg tcgcttgcct ttggcgcatt ctcattttgc gtaccgatgc
    30841 cagccagaaa accaccagaa ttaataccct gttgccacgt agccataaat ttacccacgt
    30901 ccagttaaag tgtaagccag cgccttatcc attaatcccc gagcaatttc gtggatcgtt
    30961 ggcgcaactc ctacctctga ttttttacgc tgctcctcct gaattttctt tatcgcttcc
    31021 atttgcttct cacttatcaa tactggcttt actgaaatag ccataacgcc cccttagaac
    31081 tgtataaaac aacagtcatt atagtttcat aaaacgcaac attcatcatt gttattgcaa
    31141 aatatgaaac aagcagcaca aaaaaatgcc accgttcagt agctggtggc atcgttctgt
    31201 atgtcaggtt ttgacagttt ttttatggac tctaaaaact atatttatgg agtcgcatta
    31261 ctcttcatgg cgtgagcttt cctgttctct ctttgttcct tgttccatgg ataagtgtaa
    31321 ttgccaccag caaagaccat attgattttg tgaggaacca ttaaagcgat atcagcactc
    31381 atgtcactga atgaaacaca gtgcaggcgt tcaagccatt tgtatgctgc tgccgcttct
    31441 ccccacaaat taatccctgc aagttcaaca atatctctga tggcgcagat atcaaccatc
    31501 ccgttaacgc ctgtaaaacg gcgtttaaag tcgccaagat actttctgaa aagagttgct
    31561 ttcattggtt cagaaaggtc tatgtatggg aacacaacca catcaagcag cgtctccctt
    31621 ctgttgttca tcggtattac agatggcaaa tcggatactt tgccaaatat atgatgaaaa
    31681 gcctttgcga ttatcttcat catcaatctc ccactgaaac cagttgatgt ggtgttcaga
    31741 tttcgtaaaa cactctctaa tgccagccag gtgttgtgca catcaattca gtcacattgt
    31801 gttggttcaa aatgccgtgt tgttggttca gtgttggttc aataaaaaga ataaaatcct
    31861 tataaaacaa taatctttac aaattgaacc aactgaacca actgaaccaa caccttttct
    31921 acgcacatga agtagctcat tcttcagatg agttgtaatc tcctggctgg taattgagta
    31981 cataaacgtt tatttgtcgt ccctgaatac gtggtgattt tctttggtat ccacgaccac
    32041 tgtttggtgg ggttagcatc ccggcttttt tcaacacttc agcaaactgt tttgcgttga
    32101 aaccacatgc gatctccttt tcaaatgtgg ccgggaatgt gtagaaaatc attgggcttt
    32161 cgtcatgttc tccacgctga cggtatccgg ctaaatcttt gatgggcaaa tctgccgggt
    32221 tgtatggaaa tggtgcaaaa cggctaagcc cataggcatt aagaaatgcc tccgtttgtt
    32281 caataatctg ctgatgctct ttgttacctg tgccgaactc gcgcaaccat gcgttatagc
    32341 tgtgctgtat cgcatcacgg catgtctgag catcccagcc tgttacaacc tcgcctaaca
    32401 acaatgcggc ctccagaatg gcaaatcgcg cggctacacg gtgaacctgc tcaccataat
    32461 cggatgggat caggcttctc catctgcttt cgcaatcacg aacagtttta atagcctgtt
    32521 gctggtggtc tgccagccat ttaatccact cacgcccggc tgcgccatgg tgattctggt
    32581 aagcgtcttt cagtgcgtct gcgtgctgtt ttccgttctg gtaatcgtga aagcgtactg
    32641 ctttactcaa cggaatattc agcagtcgca ccagttgccc ggctttggtt tttcgcccgg
    32701 aagtagcgat aaatgtttct aaatccattt ctccggtgct gattgccact gtacgccagc
    32761 gttttagatc ccggtttcca ccatctttag cgccttgcag tttccctaca ccgttaaata
    32821 acgcataggc agactgtgaa acactgacag gatcagcgcc ctgaccaact tcatcaagcg
    32881 gcattagtcc gtcattgtgg gcggcggctt catttgccag ccccagcgcg gttccgtacc
    32941 acgtcagccg cagtaaatca gggttgccgt ataagctgct cgcgacgttt gccgtggtgg
    33001 tcttacctgc gctcgactgc tcatagaaat gaatgccgaa cccgtcagcg cccaccagcc
    33061 caatcaatgg cgctgccagt gctgcgccga tcccggtcat cattgaatag ttaccaaagg
    33121 ccagacgcgc cacgttatca cgccagcttt tcgcgctccc tgagacggta tatccggctg
    33181 cggctgagct tctcccgctg aacagcacag gattttcagg tgttccgatg atttccccat
    33241 caggcatgat gtacgctccg cattgccagc cagtggcatg agcaacacgc cacaattcac
    33301 gagagccact gcgttgtagc cagtctgcca gtattgctct gaggctgctt ttggtcgtga
    33361 catttacccc accagcttta agtgtgcgcc atccttcacg ttcaccaata tcagccagtg
    33421 gaatggcagc ggttgtcggc gtttcagaac caaacgacag ccagcgaaga atgagatact
    33481 ggtctttatc gtctctgcca gtaccgataa cgcttaacgg agaacataac cagctttcgt
    33541 tattgatgat ttctccactc tggctgtcag ctctcggaga tacccaataa ataccatcct
    33601 cgcggctttc tattcttggt ttcagtgggt cggtatccat aaccttaaaa tcgcttccct
    33661 caatggtctg tggctcctgt ttcacgccat taccctgtgg ttggtacatt gacttattga
    33721 acgcttctgt ggcgcattta atgccgtgtt tttggtggta atcattccag tctgctttgt
    33781 gatctgtcgg gggtaatgcc acccatccag aaacagataa agctgctttt tctgctgcta
    33841 ttctccccgt gttttgtctt ccttcgctgt ggtcgttatc cgcagcaatg atgatttgtg
    33901 ctgaaggaaa tttatcccgc agtgcctggg cgacatataa caggttcccg gcatcaatcg
    33961 ccgccactgt cagcgcctct ggacgaatta aatggacaga taatgccgtt gccagccctt
    34021 ctgcgattaa gatgctctgt gttgtttctg gtgcgtttat ggcgtgatat gccccccgct
    34081 ttgctgaacc tgtcaggagt cttttttcac catgtgaggt aatagtctgt gctgctgtga
    34141 ctgcgccgga tttatccacc agcgccagca acagagatcc atcgggcaac acggggaatg
    34201 taaaatcacc tacccctttc gcaaccagat aatcagattt gccgtttaca gcctttctgc
    34261 gcatgtcatc aaactgacgg gaaaacgtat cccgtctttg ttgttcgtcc ttctctgccc
    34321 ttttcaggcg ctcctgttcg cgtcgctggc gctcggtttc cagttgttcc cgtttctggc
    34381 tggtggcttc tggtgtttcc gttgtccggt aatcaatacc cagaacatca gcggcaagaa
    34441 gcgccgcctc tgttgtgtcg cagttattta cgcgtttaat taaatccagc ccatcaccag
    34501 cgccgcactg attacagatg aagctaccgc gtccgttgtc atcgaaccgg aatcgatctt
    34561 tgcccccaca tgcagggcag ggagcatgac ggcgcggaga gtcagggaca tttatgttca
    34621 ggcagcccag cacatgaggc cagttgtttt cagacgcact gataacctga cggataagat
    34681 caatgtttcg cattctggcc cccgtttagt gcgtcactgg tgcttctggg atgccgtttt
    34741 cgtttagttc ggcaataaag ctgttgtgaa gatcggcaag cacttctcgc ccaaacgaag
    34801 tgagtgttcc ctgttcgaca tcgaacatgt tgctgtaaaa aatgatggca ttctttgttc
    34861 cgacatcaac accatatttc tcgatcattg cgccctcaat gttgtttgcc atagcgaggc
    34921 gctctgctac tggatacagc ggcatggcga cgatgccatt ggaataaaca gaaaccacgg
    34981 aatgattgcc actgtcatca ataatgctga cggtgccgtt ttcctttctc atttcattga
    35041 tgaatgctgt tgctatcagc cagcgccaca taatcacgtg atgctgtgat gtgtaatcga
    35101 aatatccctg gttgccgcca ctggcaatag cgagatgtat aaacatgcct tcatctggtt
    35161 tatcgtcata atccccggca tccagacgat ttacggcatc aatatatgag acggtctcaa
    35221 cgcgttctgt catgccatcg ttgctgcggt gcatgatgtt tacgccgtca ggcgttgctt
    35281 ctgcatggaa tgttaagaaa ttattcatgt ttcgcctcgc ctgaatgatt ctgtaactca
    35341 tatttgccca acaattcgtc cgcttcctga atagcttctg gtacgccatt gagcagtgac
    35401 ataaccgcgc caatcattaa cttgtctctg ttgctggggc tggccccttc aagccagaac
    35461 cctaaaacag tctgaacctg ttcgatgcga cattgcgctt caatgagctg gatgttggtc
    35521 atttcgcact ctccacatcg attaaaccga tatctatcag ggaggtaact ttttgtagcc
    35581 agctattgat tactgtcgcc tgttcccctc ccaccgaggg aacaatctca ttcatcacat
    35641 gaaccagacc tgtgcgcgct ctggtaagcc gttcagtcgc gcaatctgcg ggagtgaatg
    35701 tgtctggata tggctcttca cgccccagca atgattcaat ttccagctca cacggatgac
    35761 gataaattaa gttgatattc atttttcacc cccaagaatt accagctctg cacgggcatc
    35821 gttaaccaaa gatgagcacg ctttaatcgc atccacgaca tcagcagcgt ccatttcttc
    35881 agctgaaaga ctgacagcaa ccaggacagc cgctgcttgc tcgattaatt gaagtgcgga
    35941 cgtaaggcga ggggtattaa ttgagttatt cattggttgc ccccgttttt cctggcattt
    36001 cgccaccttc cagcaggaaa tgaacgtcac gaacggcgag atccaatatc gcgagtaacg
    36061 ccttcagtct ttcgtctcgt gagggagagt tgctcaccag aatgtcgtta atcacttctg
    36121 tcagtgtgat gatctggctt gtgcgttcga ggggatccat atgcacatcg taggttttaa
    36181 tcatgtgcca cctccatgcg gatacgacca gcaaaaaagc agacgtgatc gcgcaccaga
    36241 gaacggcgag catcgcgttc agactcggca gcaatatgat gaattttggc ggtaattgtc
    36301 tggcaatcgc ggcgaactgc ggcgataatc cagataaatt gcggtttttg ggtaggggta
    36361 gtagccagca tgtggcagcc tccttcagat agcaggttac gctaccaccg gaaacgccaa
    36421 tttcgctggt ggtagcccag acagggttgg cgtaaccggc tctgaaggat accggcgctt
    36481 ccgaagaagc ccccgcctga gccaccattg attgacaaag gcgctggatt atacaccaac
    36541 gcccaaaaaa tgggtgagtc agacctacga cataaaaaaa gacgctcggc gcgtcatatg
    36601 tcgccttcag aattttcagg acgccaatcc cggcacccgt tttatgaggt gccttataac
    36661 cataaaccgt gttgccgcat aacggcaagc tcttttttat ggactccgaa aactcactat
    36721 tggaagaacc aaaagcacag tacatcgcgc tcatttcgtt aacgtcacgc gcgcggatga
    36781 aggtttttgt ggagtcacta cgcgagcaga gcaggtcatc tacgaccaga aattctctgg
    36841 cgcagctgct gaccttctca acgctaacat catcgaccgt aatttctgac atagttaagg
    36901 catccgctcc taccaccagc aaatgataaa ctttgcttgt atctgctgta ttaatgcctg
    36961 actttgccgc catattgctg gattttggcg atatgccagc cagtgaaaat atcttcttct
    37021 ggacgggtat cattggttaa cctccgctaa ctccttcatg aaacgcgcca gcggttgtac
    37081 gcatggcgat tgatacccgt cgcgataaaa cgtcactcgg ttgtgagaca cgcttttgac
    37141 agtaacgagt gctccgtggg catctttgta gcgtttttga ggttgtggtg ttcctgttgg
    37201 cttagacatt gaccgcctcc agacgcttgg cgagccagcg ttgcgacagg cgggtgagtt
    37261 ctcctttgcg ctgctcatat tccatgccca tatcaattaa cgtgatattg gtgctttcga
    37321 gatatgagag gtgctcaagt tgttctgcgt tcatatggtc gcgaggttcg ccagaatagc
    37381 catttatccg cgcccactgc ttagcagtta gcccaccaag aacgatacga gaaatcatat
    37441 tgctctcgtt tgtgtagtgg tgttgctgcg tcgttttccc ctgctcggca cgggccatat
    37501 tcagcgcatc gcacattggc ttaaagttgt ttgcggcact gatacgggct tttagctggc
    37561 gacgatagcg ggcggcgatt tcaggcacgc tacgctgtaa ttcttcctcg cactggatga
    37621 aataacgacg gatagcgcga ccctgatcat tgcgttcgat cattgccagt tctttcgcca
    37681 ttccgacact taacagatag tctttgccgg gtctgccaga ctgcttaatc ttgctgtaag
    37741 ccgcgccatt cgggcttttc cccaaatttg gggaaattgt tttatggact gagtaatcgt
    37801 gcccaatggt gaagtcatat tcagagatgc gatcagtgat ccatgtagag aagtcttttc
    37861 ccacgcccaa cgctttgtgt aacgcttttg cgctaacaat attggtttca cgcccaccga
    37921 tatgaccagg aataaccggg acaattgcgg caaattcatt accgttaatt gtgcccagag
    37981 aggcgttcag atgaggggca tcctcagaaa ttaatctgct tttttcgatg ttcattttca
    38041 gtctccgtta ggcggcgtta aacgcgtccg ggtagagttt cagaatgtcg gcaatttcct
    38101 gctgagaaag tccgttgtga tcatttatgt tcgcaagatg gttaacatga gtgatcactt
    38161 tcagaacatc accacggcag gagaaacggt agcgaaggtg tgttccgata ccatcaggat
    38221 tcttttcgtc gatacgctcc agagtgattc caagctggcg ttctaactct gttgcatagt
    38281 tacgaccaga agacagacgg cagtagcgaa gaatgtcatt ttctgtccac ccctctatac
    38341 ctgtacgcag catatagact cgggcacgat gctttttcgg tgtgcgcttt ggtgcttgaa
    38401 gggattttgt aaggggcgta atatcagata cgcgaatatc tgaattagcc gcctgtaatg
    38461 ggcggttttt cttttccatc aggccacctt cccgcggcgt tctgccagcc agttgtgaat
    38521 ctcaaccgca tcaaacgcag ttattttttc tcccagcttt accggacgtg gtagtgaacc
    38581 gtttcttact ttacggtcga tggtggatac actaacgccc agcaattcag ccaggcggaa
    38641 gcgtctgata tagccttttg taggaatagt gtcggtgttc atgcttcccg taacctctta
    38701 gcgatagttg ttgcttcgtt acgggaggaa gtatctgtac atatatgaac aataaaaagg
    38761 cacagtaatg atactgtgcc tgaatagtta gctactctac cccttaccgt tggcggcagc
    38821 acgtaagtgt ctcaaaatca catcttcatt tgtgattgct aacggtggaa tttcgttttt
    38881 atgagccact atgcaatccc gccacttctc agggcttatt tctttctttt tgccacggca
    38941 ttcatcagga tactttgaca aaagatatat tgctgatttg aaaagttttt ccctgttttc
    39001 agcatgtctc tcggcattat ggttgctatt ttttatttca ggtattagtt cctgttctac
    39061 cgccatttcg tatcgttttt tgctcttagc tgcgtcaaaa cttccattaa cctttgcgtc
    39121 atgaaggtat tctaaatcag cctttgttat ataaatgttt ttatctgaca ggctactcag
    39181 gtgaatttgg gaactttgca ttttgaattt atatagatca caatcaaatg ttgcttcaag
    39241 tcgcacatat ggttctccac attcttgtga atgataatgt cgtgttgttc taccgcgtac
    39301 acgcaacaat ccacatgcaa ttgattttct tacaataaaa gagtttttgc caatttgttc
    39361 aaaaacattt ttattgtcgc tgtcttcaac tgtcagtggt ttatgtgcgt aaaatagctc
    39421 ataagcaccg ctttcagatg ctgggatata aacttcgttg cttgcgctgg taataagaaa
    39481 aggaaatgta aaccttactt cttctgggta cataagtaat aaatcagtta taaaattagc
    39541 atcttttttg aagtggatgg tggcatcaga ttcaatgaaa tcaagaaagt cagcatagac
    39601 ataaaagtct atatgtagtc tatctttcca atgccatata tcatcaattt cacaatttaa
    39661 taattttgat gctcgctcta tcgtgcagta gtccaaaggt ggaatacctg attcctgcca
    39721 taaatttcta tgcaccgcag ttgtttcatg agagtcgttc attgcgtcac cttttacgct
    39781 gcgccttatt ttgtgattga gccaggcggt aaggcttccc gcttttcgcc ccgtcgagct
    39841 aggctcaatc tattcagtta gttcgccctt ctgagaggcg ttacgttgta gtcttttccg
    39901 ctttccagtg ccaccagcat atcagcccac tgtgccagtg ctgctttacg ctcatcaaaa
    39961 tactgatggc ggttataaat cccctctacg cccttaatgc gatgattgag gcatcgttcc
    40021 gccacaacag gatcaacacc caacgctgct aaatgagtac gtgcggtgcg tcggaagtcg
    40081 tgaatcgtga aattaggcac atccggcatt tcggcgcgaa ccttagccag tgctacgggt
    40141 aaagtgcttt cctgaatatg tgggatcatt ctgttttgca tcttcctggc cggaagcacc
    40201 catgcgctat tacatgaaaa ggtgtgtagc tctctcaacc attcaacggc tggggaaggt
    40261 agaggtatat caatagggtc tccgtttttg ctgcgttctt ccggcaaatg ccataccgca
    40321 ccatctaaat caaactcttc ccatcgtgcg gcgcataact ccatcttacg aacgcatagt
    40381 gccagcaata gcttaaatgt cagttcgttc tggcggctga atccttttgc ggtgcgcata
    40441 gcctcgaata gcctgataag ctcatcgcga gttagccaac ggtcgcggga aacctcttta
    40501 ccaccagcat cagatacttc aaacgctgaa caagggttta tctccagagc gtggcgtttt
    40561 attccatagt caaagatacg gcgtgtccag cgtaatacat ctgttgctat cgttggtgct
    40621 cctcggtcaa tgataccttt caacatgtcg tcaatgtggc gtggcttaac gtcctctacc
    40681 ttcatatgcc caatgcaggg gttaatatcc ttatcaattc tccggcgaag gatatcggga
    40741 tgtttccatc tcggaagtat ctggcgctcg aagtattcgg aggccagatc tgaaacacgc
    40801 atggcgtttt tctcagcctc catttttgcc agtgcttcag cttttcgttc ctgtttctct
    40861 gctgctacgt catagccaag agcaactctg gctgacagct cttttgctgt ttctctggct
    40921 tttgatagtg acagttcgga gtaagagcct ataaccatag cccgctgctt tcctgcgagc
    40981 ttatagcgga atcgccagaa cgggactgtg tagtttttgg ggtagcaaat ataaaggccg
    41041 ttgccgtctg ccttgccatc aaatctgtct cccgccttta tccatgcgcg gatctgcata
    41101 tctgtgagtt ttggcattgc gaatacctga atatcgaata tgggtgtgta cataaagcaa
    41161 tgtacatgaa agtcattatg tacacctcta tgtacacgca tattgtgcgc tttgttgcgt
    41221 ctttatgata gctcatgata acaaaaaagc ccgacatttc gcgggctttg tggttttttg
    41281 ctgcttggtg atttttactg atagcttatt cgatattctg aatctgctcg cgcatctgct
    41341 caatcaacac tttcagctcg atggcggagt ttgtcacttc ggcgttgata gatttcgacg
    41401 caagagtgtt cgactcgcgg ttgaactcct gcatcataaa gtccagacga cgaccaacgg
    41461 cttctttttt cttcagaatg ttgtaggtct ctttgacatg cgcttcgagg cggtccagtt
    41521 cttcggcaac gtcaattcgt tgtgccagca gaaccaactc ctgctccaga cggttgtttt
    41581 ccagttgcac ctgagcatct tccagcttcg ctatcagacg ctcacgctgc cattgcagga
    41641 tttccggcat atgggcgcgg actttggcca cttcggcggt gacgccttcc agacgctgct
    41701 caatcaacac tttcagtgcc tgaccttcgg tttcgcgcgc gatgataaag tcatccaggg
    41761 tgccatccaa cgccgcgaga atttcagcgg caatcgcgtc gagatcctgc tcctgggctg
    41821 ccatcacgcc cggccagcgt agaatatcaa ccgggttgat ttccccttcg tcactctgca
    41881 tttttaccca gttcgcggca gttaccagct gtttagccag tttttcgttg aggatcagct
    41941 caccttgcgc gctaacatct ggctcatagc gcagggtaca ttccacttta ccgcgcgtca
    42001 ggcgagaacg aatacgctca cgaacgacag gttcaaggct acggaactgc tccggcagac
    42061 gaaagtaagt ttccagataa cgctggttta ccgagcgcat ttcccaggtt gcgctccccc
    42121 attcaccctt gatttcacgc cgggcgtagg cggtcatact gcggatcata gacgttcctg
    42181 tttataaaag gagaggtgga aggattatag ccatcgatgc cttgtcagga taggaataac
    42241 cgccggaagt ccgtataatg cgcagccaca tttgtttcaa gccggagatt tcaatatgcg
    42301 tccagcaggc cgtagcaata atcaggtgcg tcccgttacc ctgactcgta actatacaaa
    42361 acatgcagaa ggctcggtgc tggtcgaatt tggcgatacc aaagtgttgt gtaccgcctc
    42421 tattgaagaa ggcgtgccgc gcttcctgaa aggtcagggc cagggctgga tcaccgcaga
    42481 gtacggtatg ctgccacgtt ctacccatac ccgtaacgcg cgtgaagcgg cgaaaggtaa
    42541 gcagggtgga cgcacaatgg aaatccagcg tctgatcgcc cgtgctcttc gcgcggcagt
    42601 agatttgaaa gcactgggtg agttcaccat tacgctggac tgcgacgtgc ttcaggctga
    42661 tggtggcacg cgtaccgctt cgattacggg tgcctgcgtg gcgctggcag atgcgctaca
    42721 gaagctggtg gaaaacggca agctgaaaac taatccaatg aaagggatgg tagccgcggt
    42781 ctctgtcggg attgtgaacg gtgaagcggt ttgcgatctg gaatatgttg aagactctgc
    42841 cgcagagacc gacatgaacg tagtgatgac cgaagacggg cgtatcattg aagtgcaggg
    42901 gacggcagaa ggcgaaccgt tcacccacga agagttactc accttgttgg cgctggcccg
    42961 agggggaatc gaatccattg tagcgacgca gaaggcggcg ctggcaaact gatttttaag
    43021 gcgactgatg agtcgccttt tttttgtctg tagaaaagta agatgaggag cgaaggcatg
    43081 aaaccatatc agcgccagtt tattgaattt gcgcttagca agcaggtgtt aaagtttggc
    43141 gagttcacgc tgaaatccgg gcgcaaaagc ccctatttct tcaacgccgg gctgtttaat
    43201 accgggcgcg atctggcact gttaggccgt ttttacgctg aagcattggt ggattccggc
    43261 attgagttcg atctgctgtt tggccctgct tacaaaggga tcccgattgc caccactacc
    43321 gccgtggcgc tggcggagca tcatgacctt gacctgccgt actgctttaa ccgcaaagag
    43381 gcaaaagacc acggtgaagg cggtaatctg gttggtagcg cgttacaagg acgcgtaatg
    43441 ctggtagatg atgtgatcac cgccggaacg gcgattcgcg agtcgatgga aattattcag
    43501 gccaatggcg cgacgcttgc aggcgtgttg atttcgcttg atcgtcagga acgcgggcgc
    43561 ggcgaaattt cggcgattca ggaagttgag cgtgattaca actgcaaggt gatctctatc
    43621 atcaccctga aagacctgat tgcttacctg gaagagaagc cggaaatggc ggaacatctg
    43681 gcggcggtta aggcctatcg cgaagagttc ggcgtttaaa gaaactcgcc ggatgaaaag
    43741 tcatccggcg tcatattact gcaactgtgc ggcaattagc ggccagcggg cgtcaaaatc
    43801 atccgtcggg cggtatttaa actcgctgcg gacaaagcgt gacagcatac cttcacagaa
    43861 ggccaggatc tggcttgcca gcagggtttc atcggtggtg taaccttcac cctcacgcat
    43921 tctcttttcg cgcaatacct gacgcaactg cgcttcaata cgctcgaaca gctggttgat
    43981 gcgcccttgc aggcgatcct gttcaaacat tagtgcatga ccagtgagga tgcgggtcag
    44041 gccaggatta cgctcaccaa aaccgagaag cagcaacaca atcagacgca ggcgcgctgt
    44101 ggtgtccttc tcatctttca gaatcagatt gatgcgagta atcaggctat cttcgataaa
    44161 ctcaatcagg ctatcgaaca tgcgggtctt actggggaag tggcgataca gtgccgcttc
    44221 ggaaacgccg acagaggcgg ccagttttgc cgtcgtgata cgttggcttc catcgctgga
    44281 ttccagcatc agcgccagag actgaagtat ttcctcgcga cggttccttt tcgcagtttg
    44341 tttttctgcc atgttacaaa atacccctga aaataagcac ttgccaggcg gcacccacgc
    44401 tatgaccgca aacgaaatgt ttgcggctat gttatgacgt tattcggatg cgtatgtgtt
    44461 actgacgacc agagtgacca aagccgcctt caccgcggtc ggtggcgtcg aaatcttcca
    44521 ccagattaaa ttcagcctgt actaccggaa caaaaatcat ctgggcgatg cgttcgccag
    44581 gttgaatggt gaagctgtcc tgaccacggt tccacacgga aatcatcaac tggccctgat
    44641 agtcagaatc gatcaatcct accaggttac caagcacgat accgtgctta tgtcccaatc
    44701 cggagcgcgg cagcatcatt gccgccagtg aaggatcggc aatatgaatc gccagcccgg
    44761 tcggaaccag cgtagtgtca cccggagcca gttctacggc gtcgtcgaga caggcacgca
    44821 ggtcaagtcc ggcagagcca gaggtggcat aagtcgggag cggaaattcc ttcccaacgc
    44881 gcgggtccag aatcttaacg tcgatttttt tcatcataac gggtcacgat ctcgtcgagt
    44941 aataattggc caaggagctc tttgcgctca agcggtaaga ctttatctcc gtcctgccag
    45001 aaaaggtgta atgcgttgtt gtcgctgtta aatccttgag ttggctggga aacatcgttc
    45061 gcgcagatca gatcaaggtt tttacggata cgtttttgcc gggcgtattc ttccacatta
    45121 tttgtttcgg cggcaaatcc aacgacgtag gggcgatggt cttttagtgc ggcaactcct
    45181 gcgacgatat cggggttttt aaccattttt attgttaatt catcaccctg cgtggcctgc
    45241 tttttgattt tttctggggc cacggtagct gcgcgataat ccgccacggc ggcgcaaccg
    45301 ataaaaatat tttgctgctg tacagatgca ttcacggcgg cttccatttc cagcgcggtc
    45361 atcacatcaa cacgtttaac aaacggtggc gtcggtagtg aaaccggacc tgataccagc
    45421 gtgacgttcg cgccacgacg ggcagcggcg gcggcgatag caaaacccat cttgcctgag
    45481 ctgtgattag agatataacg caccggatcg agcggttcac gcgtcgggcc ggcggtaatc
    45541 ataatgttca gatgtttcag gtcgttgacg ggcgaaaaat gcgctaccgc catatcaaca
    45601 atggttaacg gatcgagcat tcgcccaggg ccgatatcac cacaagcctg actgccactg
    45661 tctggccccc aaatgagcaa accacgggaa gcaagcacct ctaaattatg ctgcgtggca
    45721 gcggcacggt acatctgctg gttcatggcg gggagcacgg ctacaggcgc aggtgtggcc
    45781 agacaaatcg tcgataccag atcattcgcc attccggcag caacacgggc aatcaaatct
    45841 gccgtggcag gggcgagaat cactaaatca gcccatttac ccagctcaat atggcccata
    45901 gcggcttcgg ctgccgggtc cagcagactg tcggaaacgg gataaccaga aaccgcctgc
    45961 aagctaagtg gggtgataaa ggcttttgcc gcttcggtca tggctacgcg gacgtcggcc
    46021 ccgcgatcgc gcaaacgacg caccagttca ggggttttat aggcagcaat accgccgcta
    46081 acgccgagaa cgattttttt accggccagg ctcatgatga tttttcctgt tgggtgacac
    46141 cagaagttgg cgattttatc acaatacttt tgttgtcgtg ccttcacctg agaatcactt
    46201 tgcgaggtgc tttccaggat tgaaaactgg ccgtcgattt aacggaacgg ctatgacagg
    46261 atgcgagcac cacaaaggag gtgaaggtga aaaacaatgc acagctgttg atgccgcgcg
    46321 aaaaaatgct gaggtttggt attagcgcct taacggatgt cgagctgctg gcgctatttc
    46381 tgcgtaccgg aacgcgcggt aaagatgtat taaccctggc aaaagagatg ctggagaatt
    46441 tcggctctct ttatggcttg ttaacctctg aatatgaaca gtttagtggc gttcatggaa
    46501 ttggcgtggc gaaatttgcc cagttaaagg ggattgctga actggcgcgg cgttactaca
    46561 acgtgcggat gcgtgaagaa agccctttac tcagcccgga gatgacgcgg gaatttttac
    46621 aaagccagct cacgggtgag gagcgggaga tctttatggt gatctttctc gactcacaac
    46681 accgggttat aacgcatagc cgtctttttt ccggcacgct aaaccatgtt gaagtccatc
    46741 ctcgggaaat tatccgcgaa gcgataaaaa taaatgcttc ggcgctgatc cttgcacata
    46801 atcacccttc gggttgtgct gaacccagta aagcggataa actcattact gaacggataa
    46861 taaagagttg tcagttcatg gatttacgcg tgctcgacca tatcgtgatt gggcgtggag
    46921 agtatgtttc ttttgccgaa cgcggctgga tttaacccgc tatgcgcgat ccttcgggat
    46981 ctttgtctgt tcgggacttg agcacatcgc tgagtcagcg tatactacgc cacctttgag
    47041 aatctcgggt ttggcatttg ggcctggcaa tcgagagttc acatagaact gcgatgaccg
    47101 ggctgtaaag cctgacgagg cgccaatacc ccatacgaag ctcgagctaa tttgattttt
    47161 ggagaataga catgtcccga gtctgccaag ttactggcaa gcgtccggtg accggtaaca
    47221 accgttccca cgcactgaac gcgactaaac gccgtttcct gccgaacctg cactctcacc
    47281 gtttctgggt tgagagcgag aagcgttttg tcaccctgcg cgtatctgct aaaggtatgc
    47341 gtgtaatcga taaaaaaggc atcgatacag ttctggctga actgcgtgcc cgtggcgaaa
    47401 agtactaagt acttagagga aataaatcat ggctaaaggt attcgtgaga aaatcaagct
    47461 ggtttcttct gctggtactg gtcacttcta taccactacg aagaacaaac gtactaagcc
    47521 ggaaaaactg gaactgaaaa aattcgatcc agttgttcgc cagcacgtga tctacaaaga
    47581 agcgaaaatc aaataatttt tgctttgatg taacgaaaaa cctcgccccg gcggggtttt
    47641 ttgttatctg cctgccccca tattgactgc atctgttcat tcctggagat gctatgcctg
    47701 aattacccga agttgaaacc agccgtcgcg gcatagaacc gcatctcgtt ggtgcaacca
    47761 ttcttcatgc ggtggtgcgc aacggacgct tgcgctggcc ggtttcagaa gagatctacc
    47821 gtttaagcga ccaaccagtg cttagcgtgc agcggcgggc taaatatctg ctgctggagc
    47881 tgcctgaggg ctggattatc attcatttgg ggatgtctgg cagtctgcgc atccttccag
    47941 aagaacttcc ccctgaaaag catgaccatg tggatttggt gatgagcaac ggtaaagtgc
    48001 tgcgctacac cgatccgcgc cgctttggtg cctggctatg gaccaaagag ctggaagggc
    48061 acaacgttct ggcccatctt ggaccggagc cgcttagtga cgatttcaac ggtgagtacc
    48121 tgcatcagaa gtgcgcgaag aaaaaaacgg cgattaaacc gtggctgatg gataacaagc
    48181 tggtggtagg ggtagggaat atctatgcca gcgaatctct gtttgcggcg gggatccatc
    48241 cggatcggct ggcgtcatca ctgtcgctgg cagagtgtga attgttagct cgggtgatta
    48301 aagcggtgtt gctgcgttcg attgagcagg gcggtacaac tctgaaagat tttctgcaaa
    48361 gtgatggtaa accgggctat ttcgctcagg aattgcaggt ttacgggcga aaaggtgagt
    48421 cgtgtcgggt gtgtggtacg ccgattgtgg cgactaaaca tgcgcagcgg gcaacgtttt
    48481 attgtcggca gtgccagaag taattcatgc gcgccggatg gcataccatc cggcataaac
    48541 gctacgctaa cttcgccatc agcgcctgat ggacattctc cggcaggaaa tgggtgacat
    48601 cgccctgatg gcgcgccacc tctttcacca acgatgaaga gataaacgac cactctttcg
    48661 acggcatcag aaacacactt tccagttccg gcattaagtg gcgattcata tgcgccagct
    48721 gcatttcata ttcaaaatcc gccaccgcac gcaggccacg aatcagcacc gtagcgtgtt
    48781 gattacgggc gaagttcgcc atcaaatcac taaacccgac cacttccacg ttccccagat
    48841 gcgcggttgc ctgctgtgcc agcgccacac gctcttccag agtaaacatc ggttttttac
    48901 tggggctggc ggcaatcgcc agaataacgt gatcgaacat ttgcgtggcg cgcgtcacga
    48961 tatcgatatg accattggta atgggatcga aagtacccgg ataaatcgcc cgtttttgca
    49021 taacaacctc aatgcgtttt cggtggcagg taaggttcca gcagttgaag cagacgctgt
    49081 agcgcgccct ggttttgata cagtacttca acggcatgac ggccatagaa actacggtaa
    49141 tcggcgtcgg tgagtaaaga ggaaacctct tttgcaagcg acgtggcatc ggtaacggta
    49201 atcagcccgc ttgcctgctc cagccgcgcg caaatgtctt taaagttaaa agtatgcggc
    49261 cccatcaata ccggaatagc gtgtgcggca gcttccagcg gattatgccc accacgttca
    49321 accagtgaac cgccaacaaa ggcgagatcg gcaatgccgt acagtaacat caactcgccc
    49381 atcgtatcgc caaccacaac ctgcgtgctg gtggagggga cttcccctga agatcgtgtg
    49441 atatagctta gtccagcctg gcggacaagg ttaatcgcat ccgggaagcg ttctggatga
    49501 cggggtacca ggatgagcaa taaattcggg aattgctgta acaatgcctg atgtgcggcg
    49561 atcaccacac tctcttcgcc ttcatgagtg ctggtggcaa tccataccgg gcggtgtggt
    49621 gcccattggc ggcgcagcgt cacggcttta gcagccaact gcggcgttac agaaatatcg
    49681 aatttcaggc taccggtaac ggtcacctga ttattttttg cgcccaacgc cacaaaacga
    49741 gcaccatctt cttcattttg ggcggcaatc agcgtaatac gacgcagcaa gcgacggacg
    49801 aatttaccca gtttggcata acctgcggcc gagcgggcag agagtcgcgc gttagcgatc
    49861 accagcggaa ttttacgttt atgtagcgcc gcaatcaggt taggccatag ttcggtttcc
    49921 ataatcaaca ccagtttagg gtcgacttta ttcaggaaac ggttgagtgc atcgggcagg
    49981 tcatacggca gataaacgtg ctgaacatcc ttcccgaaag ccgattgtac gcgctccgaa
    50041 ccggttggag tcatggttgt tacggtaatc ggtaaatcag gataacgatg acgcagtgcg
    50101 cgcaccaacg ggatcgccgc cagagtttca ccgacggaga cggagtgcag cataatgccg
    50161 cctggtttta gcggatggcg gtaaaaaccg taacgttcac cccagcgttt tcgataggcc
    50221 ggagccttac gtccgcgcac ccagagccgt atccagatca gtggctgaat aaggtagaga
    50281 agggcggtgt aaagcaattc gagcatagta aatagctgac ttatggatgt gctggggatt
    50341 ctatgtattt agctgtggct ttaccattac ttttcccgtt tttgacttaa atagcaattt
    50401 acccgccaat tcttgctgaa catctatact ttttttgtat ttttatgcga ttcattgatt
    50461 tctgcagtac gtgcaggaag gaatcgcaga gcaacagtaa aagaatatgg ttgctaatga
    50521 ttttatttga ttgatttctt tattgaaaat caacaatgtg cttaaaaatc attcgagtcg
    50581 gattgccgcg aaagaagtga tacactagcg cgtcaatatc agtgcggtat cagtattcag
    50641 gtagctgttg agcctggggc ggtagcgtgc tttttctgct tcaccttaat cggataatct
    50701 caacaaaaag agttacttgt ggataagcca tttcgaagaa ttttgctcat taagatgcgt
    50761 tttcatgggg atatgttatt aactactccc gtcattagtt cgctgaaaaa aaattaccct
    50821 gacgcaaaaa tcgatgtgct gctttatcag gacaccatcc cgatcctgtc tgaaaatcca
    50881 gagattaacg cgctctacgg cataaaaaat aaaaaagcaa aagcctcaga aaaaattgcc
    50941 aacttttttc atctcatcaa ggtattacgt gccaataagt atgaccttat cgttaatctt
    51001 accgatcaat ggatggttgc tatactggtt cgcttattaa atgcccgtgt gaaaatttcc
    51061 caggattatc atcatcggca gtctgctttt tggcgtaata gtttcactca tttggtgccg
    51121 ttgcagggtg gaaatgtggt ggaaagtaac ttatccgtgc tgacgccatt gggacttgaa
    51181 tcgttggtga agcagacaac catgagttac ccgcctgcaa gctggaaacg tatgcgtcgc
    51241 gaacttgatc acgctggtgt tggacaaaat tatgtggtta tccaacctac ggcgcggcaa
    51301 atcttcaaat gctgggacaa cgccaagttt tccgctgtga ttgatgcctt acatgctcgt
    51361 ggttatgaag tcgttctgac gtccggccca gataaagacg atctggcctg cgtcaatgaa
    51421 attgcgcagg gatgccagac gccaccagta acggcgctgg ccggaaaggt gaccttcccg
    51481 gaacttggtg cgttaatcga tcatgcgcag ctgtttattg gcgttgattc cgcaccggcg
    51541 catattgccg ctgcagttaa tacgccgctg atatcgctgt ttggtgcgac agaccatatt
    51601 ttctggcgtc cctggtcaaa taacatgatt caattctggg cgggagatta ccgggaaatg
    51661 ccaacgcgcg atcagcgtga ccgaaatgag atgtatcttt cggttattcc ggcggcagat
    51721 gtcattgctg ctgtcgataa attactgccc tcctccacga caggtacgtc gttatgatcg
    51781 ttgctttttg tttatataaa tattttccct ttggcggttt gcagcgcgat tttatgcgta
    51841 ttgctcagac agtcgccgcc cgaggtcatc atgttcgggt ttatacccag tcgtgggaag
    51901 gcgaatgccc tgatgtattt gaactgatca aagtgccggt taaatcgcat accaatcacg
    51961 ggcgcaatgc ggagtatttt gcctgggtgc aaaaacattt acgcgaacat cccgtcgata
    52021 aagtcgttgg attcaacaaa atgccggggc tggacgttta ttatgccgct gatgtttgtt
    52081 atgccgagaa agtagcgcag gaaaaaggct ttttctatcg cctgacgtca cgttatcgcc
    52141 attatgccgc ctttgagcgg gcaaccttcg aacagggcaa gccgacacag ctgctgatgc
    52201 tgacagataa gcaaatcgcc gatttccaga aacattatca gactgaagcg gagcgttttc
    52261 atattctgcc accggggatt tatcctgatc gtaaatatag ccagcagcca gctaatagcc
    52321 gtgaaatctt ccgtaagaag aatggaataa ccgaacaaca atatttattg ttgcaggtcg
    52381 gttcagactt cacgcgtaaa ggtgtcgatc gttccattga agcacttgct tcgttaccgg
    52441 attcgctgcg ccacaacaca ttgctatatg ttgttgggca ggataaaccg cgaaaatttg
    52501 aggcgctggc agaaaaacgc ggcgtgcgca gtaatgttca cttcttctcg gggcgcaacg
    52561 atgtctcgga attaatggcg gcggcggatt tattactgca tcctgcctac caggaagcgg
    52621 cgggaattgt gctgctggaa gcgattactg caggattacc ggtactaaca actgccgttt
    52681 gtggctatgc gcattatatt gtcgacgcca attgcggcga ggctattgct gagccattcc
    52741 gccaggaaac attgaatgag attttacgca aagcgttaac gcaattttca ttgcgccagg
    52801 cctgggcgga aaatgcgcga cattatgctg atacacaaga tttatacagt ctgccagaga
    52861 aagcggcgga tatcataacg ggtggtctgg atggttgaac ttaaagagcc gtttgccacg
    52921 ttatggcgcg gaaaagatcc ttttgaggaa gttaaaactt tgcagggtga ggtatttcgt
    52981 gaactggaaa ctcgccgtac tctgcgcttt gaaatggcgg gcaaaagcta ttttctcaaa
    53041 tggcatcgcg gcacgaccct gaaagagata atcaaaaatt tactctcatt gcggatgcca
    53101 gtattaggcg cagaccgcga atggaatgcg attcatcgac tgcgggatgt cggcgttgat
    53161 actatgtatg gggtggcatt cggcgaaaaa ggcattaatc cgctcaccag aacctcgttt
    53221 attataaccg aagatctgac accaaccatc agtctggaag attactgtgc tgactgggcg
    53281 actaaccctc cagatgttcg cgtaaagcgt atgcttatta agcgtgtcgc gacgatggtg
    53341 cgcgatatgc atgctgcggg cattaaccac cgtgactgtt atatctgtca tttcctgctg
    53401 cacttgcctt tttccggtaa gaaagaggag ttaaaaattt cggtaattga tctgcaccgg
    53461 gcgcagcttc gcacgcgcgt tccacgtcgt tggcgcgata aagatcttat agggctttat
    53521 ttttcttcga tgaatattgg cctgactcag cgggatatct ggcggtttat gaaagtgtat
    53581 tttgtcgcct cgcttaaaga cattctcaag caggaacaag gactgctgtc gcaagcagaa
    53641 gcaaaagcca caaaaatcag ggaaagaacg attcgaaaat cgttgtaatt tccggagaga
    53701 aatatgagtg cccactattt taatccacaa gagatgatca ataagacaat catcttcgat
    53761 gaaaggccag cggcgtcagt ggcatcatca ttccatgttg cttatggcat tgataaaaac
    53821 tttctttttg gttgtggtgt ttcaatcacg tcagttttgt tacataacaa cgacgtaagt
    53881 tttgttttcc acgtttttat tgatgatatc cctgaagccg atatccagcg tttagcccaa
    53941 ttggcgaaaa gctatcgtac ctgtatccag atccatctag taaattgtga acggcttaag
    54001 gcattaccga cgaccaaaaa ttggtctatt gccatgtatt tccgttttgt aattgcagat
    54061 tactttattg atcaacaaga taagatcctt tacctggatg ctgatatcgc ctgtcaggga
    54121 aacttaaagc cgctgataac aatggatctt gccaataacg ttgctgctgt tgttactgaa
    54181 cgcgatgcta actggtggtc gttacggggt caaagtctgc agtgtaatga acttgaaaag
    54241 ggttacttta attcaggtgt cctgttaatt aatacactag cgtgggcgca ggagtccgtt
    54301 tctgctaaag cgatgtcgat gcttgctgat aaagccatcg tttcccgttt aacctatatg
    54361 gatcaagata tccttaatct tatcctgtta gggaaagtta aactca
//
LOCUS       DAPHCV010000013        53341 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.013, whole genome shotgun sequence.
ACCESSION   DAPHCV010000013 DAPHCV010000000
VERSION     DAPHCV010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.013"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(275..1468)
                     /gene="tyrB"
                     /locus_tag="RH033_000805"
     CDS             complement(275..1468)
                     /gene="tyrB"
                     /locus_tag="RH033_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HDV2990037.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVADAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLVTLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEI
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(1721..2800)
                     /gene="alr"
                     /locus_tag="RH033_000806"
     CDS             complement(1721..2800)
                     /gene="alr"
                     /locus_tag="RH033_000806"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDV2990038.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(2853..4268)
                     /gene="dnaB"
                     /locus_tag="RH033_000807"
     CDS             complement(2853..4268)
                     /gene="dnaB"
                     /locus_tag="RH033_000807"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDV2990039.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4351..5334
                     /locus_tag="RH033_000808"
     CDS             4351..5334
                     /locus_tag="RH033_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HDV2990040.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(5500..5742)
                     /gene="pspG"
                     /locus_tag="RH033_000809"
     CDS             complement(5500..5742)
                     /gene="pspG"
                     /locus_tag="RH033_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HDV2990041.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKVIKAPKVPKYQRYDRWRY"
     gene            complement(5876..6871)
                     /gene="dusA"
                     /locus_tag="RH033_000810"
     CDS             complement(5876..6871)
                     /gene="dusA"
                     /locus_tag="RH033_000810"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HDV2990042.1"
                     /translation="MPAESHTVYPAYRFSIAPMLDWTDRHCRYFLRLLSRNTLLYTEM
                     VTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPS
                     DRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTV
                     SGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSL
                     EEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDIDADPVAVVRAMYPYIEREL
                     SQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVLEHALKLVADKR"
     gene            7027..7542
                     /gene="zur"
                     /locus_tag="RH033_000811"
     CDS             7027..7542
                     /gene="zur"
                     /locus_tag="RH033_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HDV2990043.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALEFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCSACVEVEACRHPEQCHH
                     DHSIQVKKKPR"
     gene            complement(7584..7793)
                     /locus_tag="RH033_000812"
     CDS             complement(7584..7793)
                     /locus_tag="RH033_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDV2990044.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVDSWEKRHDYRW"
     gene            complement(7909..9234)
                     /gene="dinF"
                     /locus_tag="RH033_000813"
     CDS             complement(7909..9234)
                     /gene="dinF"
                     /locus_tag="RH033_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HDV2990045.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNDTWFVAT"
     gene            complement(9307..9915)
                     /gene="lexA"
                     /locus_tag="RH033_000814"
     CDS             complement(9307..9915)
                     /gene="lexA"
                     /locus_tag="RH033_000814"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HDV2990046.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(10025..10393)
                     /gene="dgkA"
                     /locus_tag="RH033_000815"
     CDS             complement(10025..10393)
                     /gene="dgkA"
                     /locus_tag="RH033_000815"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDV2990047.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            10564..12987
                     /gene="plsB"
                     /locus_tag="RH033_000816"
     CDS             10564..12987
                     /gene="plsB"
                     /locus_tag="RH033_000816"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HDV2990048.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(13142..14014)
                     /gene="ubiA"
                     /locus_tag="RH033_000817"
     CDS             complement(13142..14014)
                     /gene="ubiA"
                     /locus_tag="RH033_000817"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HDV2990049.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(14027..14524)
                     /gene="ubiC"
                     /locus_tag="RH033_000818"
     CDS             complement(14027..14524)
                     /gene="ubiC"
                     /locus_tag="RH033_000818"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HDV2990050.1"
                     /translation="MSHPALTQLRALRYFTEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(14703..15623)
                     /gene="malM"
                     /locus_tag="RH033_000819"
     CDS             complement(14703..15623)
                     /gene="malM"
                     /locus_tag="RH033_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HDV2990051.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLSTGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(15764..17104)
                     /gene="lamB"
                     /locus_tag="RH033_000820"
     CDS             complement(15764..17104)
                     /gene="lamB"
                     /locus_tag="RH033_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HDV2990052.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTGSSSTNPYYGKAVSADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(17176..18291)
                     /gene="malK"
                     /locus_tag="RH033_000821"
     CDS             complement(17176..18291)
                     /gene="malK"
                     /locus_tag="RH033_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HDV2990053.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            18656..19846
                     /gene="malE"
                     /locus_tag="RH033_000822"
     CDS             18656..19846
                     /gene="malE"
                     /locus_tag="RH033_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HDV2990054.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYQ
                     DELAKDPRIAATMDNAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDAALKDAQT
                     RITK"
     gene            20000..21544
                     /gene="malF"
                     /locus_tag="RH033_000823"
     CDS             20000..21544
                     /gene="malF"
                     /locus_tag="RH033_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HDV2990055.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKIYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            21559..22449
                     /gene="malG"
                     /locus_tag="RH033_000824"
     CDS             21559..22449
                     /gene="malG"
                     /locus_tag="RH033_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HDV2990056.1"
                     /translation="MAMVQPKSQKTRLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRVTPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(22543..22953)
                     /gene="psiE"
                     /locus_tag="RH033_000825"
     CDS             complement(22543..22953)
                     /gene="psiE"
                     /locus_tag="RH033_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HDV2990057.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            23168..23442
                     /locus_tag="RH033_000826"
                     /pseudo
     CDS             23168..23442
                     /locus_tag="RH033_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23489..25585)
                     /locus_tag="RH033_000827"
     CDS             complement(23489..25585)
                     /locus_tag="RH033_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HDV2990058.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQTKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGVDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(25585..26322)
                     /locus_tag="RH033_000828"
     CDS             complement(25585..26322)
                     /locus_tag="RH033_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HDV2990059.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNDTLWVGPPPSTVTLFGLISRPGKQP
                     FIPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(26319..26957)
                     /locus_tag="RH033_000829"
     CDS             complement(26319..26957)
                     /locus_tag="RH033_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HDV2990060.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSQQYLGADYFPVKTTLIKAAKQ"
     gene            complement(27144..27386)
                     /locus_tag="RH033_000830"
     CDS             complement(27144..27386)
                     /locus_tag="RH033_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2990061.1"
                     /translation="MKKVLYGIFAISALAATSVWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAASGGGDGSNTGTTTTTTTSTQ"
     gene            complement(27884..29533)
                     /gene="pgi"
                     /locus_tag="RH033_000831"
     CDS             complement(27884..29533)
                     /gene="pgi"
                     /locus_tag="RH033_000831"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDV2990062.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            30058..31407
                     /gene="lysC"
                     /locus_tag="RH033_000832"
     CDS             30058..31407
                     /gene="lysC"
                     /locus_tag="RH033_000832"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HDV2990063.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTDDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            31661..31933
                     /locus_tag="RH033_000833"
     CDS             31661..31933
                     /locus_tag="RH033_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HDV2990064.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAAAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(31934..32806)
                     /gene="rluF"
                     /locus_tag="RH033_000834"
     CDS             complement(31934..32806)
                     /gene="rluF"
                     /locus_tag="RH033_000834"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HDV2990065.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKTKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            33154..34101
                     /locus_tag="RH033_000835"
     CDS             33154..34101
                     /locus_tag="RH033_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HDV2990066.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQVKQKFSLKDVVVVSGNDEDEDTQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGTLRGKYINCLVTNSSTAELLLK"
     gene            34171..34977
                     /locus_tag="RH033_000836"
     CDS             34171..34977
                     /locus_tag="RH033_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV2990067.1"
                     /translation="MQTWLNLQDKIIIVTGGACGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSQRASYITGVTTNIAGGKTRG"
     gene            34987..35394
                     /locus_tag="RH033_000837"
     CDS             34987..35394
                     /locus_tag="RH033_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HDV2990068.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     HSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            35394..35888
                     /locus_tag="RH033_000838"
     CDS             35394..35888
                     /locus_tag="RH033_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HDV2990069.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQHIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIDTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            35954..36751
                     /locus_tag="RH033_000839"
     CDS             35954..36751
                     /locus_tag="RH033_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDV2990070.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIMLGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALLYIQLNPQWRKAEPHPQTTTITALDQLDD"
     gene            36762..37586
                     /locus_tag="RH033_000840"
     CDS             36762..37586
                     /locus_tag="RH033_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDV2990071.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            37638..38867
                     /gene="sorE"
                     /locus_tag="RH033_000841"
     CDS             37638..38867
                     /gene="sorE"
                     /locus_tag="RH033_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HDV2990072.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLMSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            39250..39939
                     /gene="pepE"
                     /locus_tag="RH033_000842"
     CDS             39250..39939
                     /gene="pepE"
                     /locus_tag="RH033_000842"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HDV2990073.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(40029..41660)
                     /locus_tag="RH033_000843"
     CDS             complement(40029..41660)
                     /locus_tag="RH033_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDV2990074.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(41880..45563)
                     /gene="metH"
                     /locus_tag="RH033_000844"
     CDS             complement(41880..45563)
                     /gene="metH"
                     /locus_tag="RH033_000844"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDV2990075.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPAEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEYYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            45763..46587
                     /gene="iclR"
                     /locus_tag="RH033_000845"
     CDS             45763..46587
                     /gene="iclR"
                     /locus_tag="RH033_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HDV2990076.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSNHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGIR"
     gene            complement(46604..48328)
                     /gene="aceK"
                     /locus_tag="RH033_000846"
     CDS             complement(46604..48328)
                     /gene="aceK"
                     /locus_tag="RH033_000846"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HDV2990077.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIVRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDRFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPLPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRK
                     QRFSVRFV"
     gene            complement(48544..49848)
                     /gene="aceA"
                     /locus_tag="RH033_000847"
     CDS             complement(48544..49848)
                     /gene="aceA"
                     /locus_tag="RH033_000847"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HDV2990078.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(49878..51479)
                     /gene="aceB"
                     /locus_tag="RH033_000848"
     CDS             complement(49878..51479)
                     /gene="aceB"
                     /locus_tag="RH033_000848"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HDV2990079.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKHILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(51748..52677)
                     /gene="metA"
                     /locus_tag="RH033_000849"
     CDS             complement(51748..52677)
                     /gene="metA"
                     /locus_tag="RH033_000849"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HDV2990080.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEFFRDVEAGLDPDVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            52834..53277
                     /locus_tag="RH033_000850"
     CDS             52834..53277
                     /locus_tag="RH033_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDV2990081.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDRPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVKHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTERSEVDDLGKPYPLLNLAYVGE"
BASE COUNT        12829 a        14173 c        13710 g        12629 t
ORIGIN      
        1 ttcttgagga tatagaaaaa ttctatcgta acaaacccga tacttattat taaatttaat
       61 aatggttaga tgtgcaaaaa ttattattcc acacggtaaa acagggaaaa gcactccaga
      121 acattgggat aaacttaaca agttagaact aacaaccttt tattacctct cacgtagaac
      181 gatggcatca aaaaaatcat ctataaagca agagtgaaag cgacgacagt ttaatttcac
      241 tgcaggctgg gtaactccag cctgctttcc tgcattacat caccgcagca aacgcctttg
      301 ccacacgttg tacatttgcc gtatttaacc cagcgacaca catgcgaccg ctggcgatga
      361 gatagacacc aaattcttca cgtagtcggt caacctgagc ggcacttaaa ccggtataac
      421 tgaacatacc gcgctgatta agcagataat cgaaattgcg ttctggtatc tctgtgctca
      481 ataccttcac cagttcctga cgcatcgcca gaatgcgagt acgcatctct tctacttccg
      541 ccagccagct ggctttcaat gcctcgtcat tcagcaccgc agccaccacc tgcgcaccaa
      601 aattcggcgg gctggagtag ttgcggcgaa ctgttgcttt caattgcccc agtacgcggc
      661 ctgcggcttc ggcatcttca cacagaacag aaagtccgcc gacgcgctcg ccgtaaaggg
      721 agaaaatttt cgagaacgaa ttgctcacca gagcgggtaa tccagcgctg gcaatggcgc
      781 gaatggcgta ggcatcctct tccataccgg caccaaatcc ttgataggca atatcaagaa
      841 atgggataag ctcgcgggct ttgagaattt caatcaccgc atcccattgg tcattagtga
      901 gatcggcacc cgttgggttg tggcaacatg gatgcagcaa cacaatactg cgggcaggta
      961 atgttttcag cgtcaccaac aggtcattaa agcgcacgcc gttagtcgct tcgtcatacc
     1021 aggggtaagt acttacttcg aatccagccc cggcgaatat tgctacgtgg ttttcccatg
     1081 taggatcgct gacccagacg cctgattccg ggaagtagcg tttcaggaaa tccgcgccca
     1141 ctttcaatgc ccctgagccg ccaagggttt gaatggttgc tacgcgctgt tgttgcagta
     1201 ccggatggtc ggcaccaaac agcagcggcg caatagcatg gcgatagctg ttaagccctt
     1261 ccatcggtaa ataaagcgaa gcgccatgag gctgcgcatt caggcgcgct tccgcatccg
     1321 ccacggcttt cagttgtgga ataattccgt cttcgttgta gtacagaccg atacttaaat
     1381 tcactttgtc gctgcgaggg tcttctttaa aacgctccat aagcgtaaga atcgggtcgc
     1441 cagcgtaggc gtcaactttt tgaaacacgc gatggttctc caggtttacg ggcaggtggt
     1501 taaaacacaa taaaccggaa gaaggcgaag atcgagtgga tgttcagggg cgaacggcaa
     1561 ttagcaacag agtgagactc atgacaaacg tacatccgcc agaggcacgg ccttcataag
     1621 agcatgaaag aatatcaact tattgaattg gtaggatttt attggccgga taaggcattc
     1681 acgccgcatc cggcacagac aatcaaatat tacagaacga ttaatccacg tatttcatcg
     1741 cgacccttga agtcaggcgc gtaataagtt cgtaagcgct cacttttgtc atttcagcga
     1801 tacgttctac gggcaaacct tcgccccata aaatgaccgg gtccccggct ttgtcctgcg
     1861 cctgtggacc taagtctacg cagatcatat ccatcgcgac gcgcccgaca atcggcactt
     1921 cgcgaccgtt caccagcact ggcgtaccgg acggcgcggc gcgcggataa ccatcgccat
     1981 agcccatcgc gactacgcca aggcgggtat cacgttcgct tacccaggtt ccaccataac
     2041 cgacaggctc tccggcttta tgctcacgca cagcaatcag gctggaggtc aatgacatta
     2101 ccggctgaca gccaaaatcg gcaccggtgg aacgatcttc cagcggcgag acgccataaa
     2161 gaatgatgcc cgggcgcacc cagtcaaaat gcgactgtgg ccacagcaga atgccacccg
     2221 acgcggcaat ggaacgttga cctggtttgc cttcacaaaa ggtattaaag atagcgagtt
     2281 gtttctcggt tgcgccgcat tttggttcat ccgcgcgcgc aaaatggctg acgatattca
     2341 ccggctgacg aacgtttttg cactgggtca ggcgatgata aaacgcctca gcctgttccg
     2401 gccttacgcc cagacggtgc ataccggtat cgagtttcat ccagacggta accggctcgt
     2461 ccaggctagc ctcttccagc gcagccagct gttcttcgtt atgcacggcg gtatgaaaat
     2521 gttgcgcaga aatcgtcggc agatctctgg catcaaaaaa gccttcgagt aacagtacag
     2581 gtttggtgat tccccccgca cgcagtcgca gagcttcttc gagacgggct acgccaaagg
     2641 cgtcagcatc ggggagcgtt cgcgcggtct caagaagacc gtgaccataa gcgttcgctt
     2701 tcaccaccgc aaccatttta ctggcaggcg ccagttcacg aagacgttgc aggttgtgtc
     2761 gcagagcgcg gcggttaatc acaacagttg ccgcttgcat ttgtgttcct tgataagtgt
     2821 ttgctttaat tacctaattc ataaaataat tattattcgt cgtcgtactg cggccccgca
     2881 tagttgtcga agcgcgacca ttgaccgtta aaggtcaggc gtaccgtccc gattgggccg
     2941 ttacgttgtt taccgataat aatttccgcg atgcctttta aatcactgtt ttcgtgatac
     3001 acctcatcac gatagataaa catgatcaag tccgcatcct gctcgataga gccagattca
     3061 cgcaggtcgg agttgaccgg gcgtttgtcg gcacgttgtt ccagagaacg gttcaactgg
     3121 gacagcgcca ccaccggcac gttcagttct ttcgccagtg ctttcagcga gcgggagatt
     3181 tctgcaattt ccagcgtacg gttatcggaa agcgccggta cgcgcatcag ttgcaggtag
     3241 tcgatcatga taagcccgat gccgccgtgt tcacgggcaa tacggcgtgc gcgggaacgc
     3301 acttccgttg gcgtcaagcc ggaggaatca tcgatataga tattacgttt ttcgagcaaa
     3361 atacccatgg tgccggaaat gcgtgcccag tcttcatcat cgagctgacc ggtacggatt
     3421 ttagtctggt caacgcgcga cagcgacgcc agagaacgca tcatgatctg ttctgatggc
     3481 atctccagcg agaagataag caccggttta tcctgcaaca tcgccgcgtt ttcgacgagg
     3541 ttcatcgcaa atgtcgtttt acccatcgac ggacgcgcgg caacgatgat caaatccgac
     3601 ggctgcaagc cagcggtttt tttgttgaga tcgtcataac cggtatttac cccggtaacg
     3661 ccatcgtgtg gctgctgaaa caactgctca atacgcgcca cggttgcgtc gagcacatcg
     3721 gcgatgttct tcggcccttc gtctttgttt gcacgacttt cggcaatttt aaagacgcgg
     3781 gattcagcaa ggtcgagcag atcttcactg gtacgccctt gtggatcaaa accagcttcg
     3841 gcaatctcgt tagcaacgga gatcatctca cgaacaacgg cacgttcgcg cacgatatcc
     3901 gcatatgcac tgatgttcgc cgcacttggc gtattttttg acagctctgc cagataagca
     3961 aaaccaccga cgctatcgag ttgcccctgg cgttccagcg attccgcaag ggtaatcaga
     4021 tcgataggac taccgctttc ctgcaaacgc gccatttcag taaagatatg acggtgtggg
     4081 cgggtgtaaa aatcatctgc caccacacgc tcagctacat catcccagcg ttcgttatcc
     4141 agcattaaac cgcccaacac cgactgctcc gcttcgatcg agtgcggagg cactttcagc
     4201 ccggcaactt gtggatcgcg ttcgcggggt tcagcctgct gtttgttgaa gggtttattt
     4261 cctgccatag tgaatggagt taccgagata aagaatgggt cgaaacttta ccatatgaag
     4321 cagaccctga cgatacgttc tggaggacac atggcaacac gaattgaatt tcacaagcac
     4381 ggtggcccgg aagtacttca agccgtagag ttcactcctg ccgatccggc ggagaatgaa
     4441 atccaggtcg aaaataaagc catcggcatc aattttatcg acacgtatat ccgcagcggc
     4501 ctttacccgc cgccatcgct acccagcgga ttaggcaccg aagcggcagg catcgtgagt
     4561 aaagtcggca gtggtgtaaa gcatattaag gcaggcgatc gcgtagtcta tgcacaatcg
     4621 gcattaggcg cttacagctc tgtgcataac attaatgcgg ataaagcggc gattctgcct
     4681 gcggcaattt cttttgagca agctgcggca tccttcctga aaggcttaac ggtttattat
     4741 ctgctgcgca aaacctatga aattaaaccc gatgagcagt tcctgttcca cgcagcggct
     4801 ggcggcgttg gcttgattgc ctgccagtgg gcaaaagccc ttggcgcgaa acttatcggc
     4861 accgtaggaa ccgcgcaaaa agcgcagagt gcgctaaaag cgggcgcgtg gcaggttatt
     4921 aactatcgtg aagagaatct ggtcgagcgg ttaaaagaga tcaccggtgg caagaaagtg
     4981 cgcgtggtgt acgattccgt gggcagagac acctgggaac ggtcgctgga ttgcctgcaa
     5041 cgccgcggct taatggtgag ttttggcaac tcatcaggtg cggttaccgg tgtgaactta
     5101 ggcattctca atcagaaagg ctcgttgtat gtgacacgcc cttccctgca aggctatatc
     5161 accacgcggg aggaattaac cgaggccagt aatgaactgt tctctttgat tgccagcggt
     5221 gtgattaagg tcgatgtcgc cgagcagcag aaatatccgc tgaaggatgc gcagcgtgcg
     5281 catgagattc tggaaagccg ggcgacgcaa ggttccagcc tgttgattcc ataaaagaaa
     5341 tagggcttcc acctgggaag ccctttcttt ttatagttcg gctgtatgta gggtacagca
     5401 cgatgaatct gttagaggcg caatagtgac agatttgatt atcaattcct attttgttct
     5461 aaggataaaa ccttaggttg tgatcgtccg cacaatccct tagtaacgcc aacggtcata
     5521 gcgctgatat ttcggcactt ttggtgcttt aatcacctta ataacccaca ctaccgcaat
     5581 cgccaataac agccacggca gcaacttaat catcaatgcc agcataccgc cgaggaacat
     5641 aatggccgtc gccacaacca gcgcagcgat aatgcccagc aacgaaacgc cggtgaccat
     5701 cagcatgaca aaaaagccaa tcacaaaaag tagttccagc atgatgctct cccaaatatg
     5761 aaatctcttg caggcattac aagaatcatg ccaaaaataa tctattgatt taacagcaaa
     5821 acgccccgcg acggtgcgca gggcgtggtg aatttgacta ctttttggtg aaaagttaac
     5881 gcttatccgc caccagtttg agcgcgtgtt ccagcacatt aatgtctgca cccgctttat
     5941 gggcattttc acttaaataa cgccgccact gccgcgcgcc aggaataccc tggaacaagc
     6001 ccagcatatg ccgggtaata tggccgagat acgtaccctg gctgagttca cgctcaatgt
     6061 acggatacat ggcgcgcact accgccaccg gatcggcatc aatatccgag gaaccaaaaa
     6121 tctctcggtc taccgccgcc agaatacccg gattctgata cgcctcgcgc ccgaccatca
     6181 cgccatccat atgttgcaaa tgcgctttag cttcttccag cgacttgata ccaccgttaa
     6241 tcgacatcgt cagatgcgga aagtcacgct tcagttgata cacacgcgga taatcgagcg
     6301 gcgggatctc acggttttct ttcggactta acccagaaag ccaggcttta cgtgcgtgga
     6361 tgatgaacat ctcacactca cctttgccgg aaacggtgtt gatgaaatcg cagagaaatt
     6421 catagctgtc ctgatcatcg atgccaatac gcgtcttcac cgtcaccgga atcgacacca
     6481 catcgcgcat cgctttcacg cagtcggcaa ccagctgcgc attacccatc agacacgcac
     6541 caaacatgcc gttctgcacc cggtcagacg ggcagccgac attcaggttg atctcatcgt
     6601 atccacgcgc ttctgccagc ttcgcacact gtgccagcgc cgccggatcg ctacccccga
     6661 gttgcaacgc taccggatgt tcttcttcac tgtacgccag gtaatcacct ttaccgtgaa
     6721 taatcgcccc tgtggtcacc atttcggtat acagcaacgt attgcgggaa agcagacgca
     6781 ggaaataacg gcaatgtctg tccgtccagt caagcatcgg ggcaatcgag aaacggtaag
     6841 cgggataaac agtgtgtgat tctgctggca tggcgacttt ttaagcatct ggtaaattgg
     6901 gggcgctact atagcataac gaatatgcga tcaggcaatg tgacagccat gacaccctgt
     6961 tctcggcgtt taagcgagaa ctatggtaaa gtaaggacat tcttaacccc actgtcgagg
     7021 tgcccaatgg aaaagaccac aacgcaggag ttattagcgc aggctgaaaa aatctgcgca
     7081 cagcgtaatg tgcgcctgac cccacagcgc ctcgaagtgt tgcgcctgat gagtctgcag
     7141 gatggcgcta tcagcgctta tgatctgctt gatttgctgc gcgaagctga accgcaagcc
     7201 aagccgccaa cggtttatcg cgcgctggag tttctgcttg aacaaggttt tgtgcataag
     7261 gtggaatcca ccaacagtta tgtgctctgt catctgttcg atcagcccac ccatacttca
     7321 gccatgttta tttgcgatcg ctgcggcgcg gtgaaagaag aatgtgctga aggcgtggaa
     7381 gacatcatgc atacgctggc ggcaaaaatg ggttttgccc tgcggcataa tgttattgaa
     7441 gcacatggat tatgttcagc atgtgtagaa gtagaagcgt gtcgtcatcc tgaacagtgc
     7501 catcatgacc actctattca ggtgaaaaag aaacctcgct aaaagggtgt acatccttgt
     7561 acatgtcggg caggagggat taattaccag cggtaatcat ggcgtttttc ccaactatca
     7621 acctcttttt ctgcctgatc tttctgataa ccgtagcgtt cctggatttt acccactaac
     7681 tgatcacgtt ttccttcaat gatcgtcata tcatcatcgg tcagtttgcc ccattgctct
     7741 ttcactttac ctttaaactg tttccagtta ccgccggctt catctttatt cataatcaag
     7801 acctcatcgt taggttgtga atgagagtac gttcactttt cttctgaacg tgagattaag
     7861 tgtagtcagc aatttggctt aggattattt attcagaatt tttaaccgtc acgttgcgac
     7921 aaaccaggta tcgttacgcc agtgacgccg ccagatagcc gccagagaaa gcccgcgtaa
     7981 cgccagaaag acggttaacg ccagccacaa accatgattc cccagccacg gcagcgtaag
     8041 gagcgtcagc gcaaaacctg cggcggccac cgccatactg ttacgcattt cggcggcacg
     8101 cgttgcgcct ataaacatgc cgtccagcag ataacaccag acgccgacca acggcaaaat
     8161 cacctgccag ataagatagc ggtcagccag ctgctgaatc tgggttaacg acgtcagcaa
     8221 cgcaatgatg tgttcccctg ccagcaaata aaccaccgaa aacagtaacg ctacgatccc
     8281 cgactggcgg cacgctgccc gccagacatc cagcaactgg ctaccgtcgc gcgcaccata
     8341 tgcctgaccg gaatgtgctt caaccgcgta ggcaaaacca tccagcgcat aggcggtaaa
     8401 ggtgagtagc gtcatcagaa ccgcgttaac agcgataatg tcactcccca gtcgtgcgcc
     8461 aagtacggtg atcgcgccga aacagagttg caacaacagc gagcgcagca tgatatcgcg
     8521 gttaagcgcc agcaagcgac ggaaatttcc tcgccaggca gttttcagca tttcgccgga
     8581 gattccgcgt agtttgagga ttttacgcac cattagcaga ccaatcagca atgttgcata
     8641 ttccgcaata accgtcgcca gcgccgcgcc ctgcacgttc atatgcagcc ccatcaccag
     8701 ccagacatcc agcacaatgt tgaggatatt gccgaccact aacaaaatta ctggcgcacg
     8761 ggcatattgc acgccgagta accaaccaag taataccaga ttcgccagcg acgccggtgc
     8821 gcttaaccag cggatttcaa gaaagcgccg cgcctgttct agcaccgctt cgctgccgcc
     8881 aacaatatgc agcgccagat cgataatcgg cgtacgcagc agcgcaatta acgccccagc
     8941 ccccaacgcc aacagcaacg gttgcaccag cgcacgggct aatgcctgag gatttttggc
     9001 accataagcc tgcgcagtca gcccggtggt gctcatgcgt aaaaacagca acaacataaa
     9061 gagaaagctg gtcgccgttg caccaaccgc cacgccgccc aaataaaccg gactatcaag
     9121 atgaccaatt accgccgtat cgaccagtcc cagcaacgga acggtgatat tggagaaaat
     9181 catgggtaag gcgagatgcc agagtgcttt atcagatgaa gtgaggaatg ccatgcagac
     9241 aagcctgatg aagagagatg aaaaacaaac cgcgatacca ggcggcatcg cggtctcaga
     9301 gatatgttac agccagtcgc cgttgcgaat aaccccaacc gccagccctt caatggtgaa
     9361 gctctgctga cgaaggtcaa cgacaattgg tttaaactcg ctgttttctg gcaacagttc
     9421 gactttattg ccctgttttt tcaggcgctt aacggtaact tcgtcatcaa tacgtgcgac
     9481 aacgacctga ccgttacgta catcctgagt tttatgcact gccagcaaat cgccatccat
     9541 aatgccgata tctttcatcg acatcccgct gacacgcagc aggaaatcag cattcggctt
     9601 gaacaaggaa gggtcgacct gataatgacc ttcaatatgc tgttgcgcca gaagcggttc
     9661 accggcagcc acacgaccta ccagcggcaa cccttcttcc tcttcctgca ataaacgaat
     9721 cccgcgtgat gcgccggaaa caatttcaat aacgcctttg cgtgccagcg ccttcagatg
     9781 ttcttcagcc gcgtttgggg aacggaaccc caaacgctgc gcgatttccg cacgcgtcgg
     9841 cggcatacct gtctggctga tgtgatcacg gatgagatca aacacctctt gttgcctggc
     9901 cgttaacgct ttcattccgc cccctgggtg tatatacagt tatgctgtga gtatatacag
     9961 caaaaggcga ttttggaacc ataaactgca caataaacca gagatttatc gaaatctgga
    10021 agggttatcc aaaatgcgac cataacagga tgcaccaggt aatcacggcg acgataatgg
    10081 caatcagcac tgcggcggat cccatatctt tagcgcgtcc ggaaagctca tggtattcag
    10141 agccaattcg gtcaaccacc gcttcgatgg cgctattgag aatttccaca atcatcacca
    10201 gcatcacgga gctgataagc agtacgcggg taatcgcgtc cacatccagc cagcaggcga
    10261 taaccaccgc taacaatacc gctacacctt cctgacggaa tgccgcttcg ttgatccatg
    10321 cagcgcgtaa acctttccag gaatagccag cagctttgat aattcgggtg aatccagtgg
    10381 tattattggc cattgaaaga acctttttac attatgagcg tcaatatcag tgtaccgtta
    10441 aatccgatga ttcgggtttg cgtaacgccg cgcgaaacat gagcggatac cacagaattt
    10501 cccatgactt tctgctatcc ttgccgcgca tttgcattat taaccagagg ctttacatcg
    10561 tttatgtccg gctggccacg aatttactac aaattactga atttaccatt aagcatcctg
    10621 gtaaaaagca agtctattcc ggcggatcct gccccggaac tggggctgga tacctctcgt
    10681 ccaattatgt acgttttgcc gtacaactcg aaagctgact tgctgacgtt gcgcgcccag
    10741 tgtctggcac atgacctgcc tgacccgtta gagccgctgg aaatcgacgg cacgctactg
    10801 ccgcgctatg tgttcattca cggcgggccg cgtgtgttca cctattacac gccgaaagaa
    10861 gagtctatta agctgttcca cgactatctc gatttgcacc gcagcaaccc aaatctggat
    10921 gtgcagatgg tgccagtatc ggtgatgttt ggtcgcgcgc cggggcgtga aaaaggcgaa
    10981 gtgaacccgc cgctgcgtat gcttaacggc gtacagaaat ttttcgctgt actgtggctc
    11041 ggtcgcgaca gttttgtgcg tttctcgccg tcagtttcgc tgcgccgtat ggcggatgaa
    11101 cacggcacgg ataaaactat cgctcagaaa ctggcgcgcg tagcacgtat gcactttgcc
    11161 cgtcaacgcc tggctgccgt gggtccacgt ctgcctgctc gtcaggatct gtttaataag
    11221 ctgctcgcct cccgcgccat tgcgaaagcg gtagaagatg aagcgcgcag caaaaaaatc
    11281 tcccatgaaa aagcgcagca gaacgcgatt gcgctgatgg aagagattgc ggcgaatttc
    11341 tcttacgaga tgatccgcct gactgaccgc attctgggct tcacctggaa ccgactttac
    11401 cagggtatca acgtccataa cgccgagcgc gttcgccagc tggcccacga cggccatgag
    11461 ctagtgtatg tgccttgcca ccgcagtcac atggactacc tgctgctttc ttacgtgctg
    11521 tatcaccagg ggctggtgcc gccgcatatc gccgccggga tcaacctgaa cttctggccg
    11581 gccgggccga ttttccgccg tctgggggcg ttctttattc gccgtacgtt taaaggcaat
    11641 aaactttatt ccaccgtttt ccgtgagtat ctcggcgaac tgttcagccg tggttattcc
    11701 gtcgagtatt tcgtggaagg cggtcgttcc cgtacggggc gtttgctgga tccgaaaacc
    11761 ggtacgctat cgatgaccat tcaggcgatg ctgcgtggcg gcactcgtcc gattacgctg
    11821 attccgatct atatcggtta tgagcacgtc atggaagtgg gtacttacgc caaagaactg
    11881 cgcggtgcga cgaaagagaa agagagcctg ccgcagatgc tgcgtggttt aagcaagctg
    11941 cgtaatctcg gtcagggtta cgtcaacttc ggtgaaccaa tgccgttgat gacctacctt
    12001 aaccagcacg taccagactg gcgtgaatct atcgatccca tcgaagcggt gcgtccggcc
    12061 tggttaacgc cgacggtcaa taatattgct gccgatctga tggtacgcat taacaacgca
    12121 ggcgcggcaa acgccatgaa cctgtgctgt actgcgcttc tggcgtcacg tcagcgctca
    12181 ctcacccgcg agcagttaac cgagcaactc aactgctacc tggatctgat gcgcaatgtg
    12241 ccttactcca cggactctac cgttccttca gccagcgcca gcgagcttat cgatcacgcg
    12301 ctgcaaatga acaagtttga agttgagaaa gacacgatcg gcgacatcat cattctgccg
    12361 cgcgagcaag cggtgctgat gacctactat cgcaacaaca ttgcgcatat gttggtgctg
    12421 ccttcgctga tggcggcaat cgtcacccag catcgccaca tctcccgcga cgtattgatg
    12481 gagcacgtca atgtgcttta cccaatgctg aaagcggagc tgttcctgcg ctgggatcgc
    12541 gacgagttgc cggacgttat tgatgcactg gcaaatgaga tgcaacgtca ggggctgatt
    12601 accctgcaag atgatgagtt gcatatcaac ccggcgcatt ctcgcacgct acagctgctg
    12661 gccgcaggcg cgcgcgaaac gctgcaacgt tatgccatta ccttctggct gttgagcgcc
    12721 aacccgtcga tcaaccgcgg tacgctggag aaagagagcc gcaccgtcgc gcaacgtctc
    12781 tccgtgctgc acggcatcaa cgcgccggag ttcttcgaca aggcggtgtt cagttctctg
    12841 gtgctgacac tgcgtgatga agggtatatc agcgatagcg gcgacgccga accggcagaa
    12901 acgatgaagg tgtatcagtt gctggcggag ttgattacat cagacgtgcg tttgacgatt
    12961 gagagtgcga cgcagggcga agggtaatca gggagaattg ccggatgcgg cgcaaacgcc
    13021 ttattcggcc taccatgatc tgcaaattca ataaattgcg atatgttgtg taggcctgag
    13081 aagcgcagcg catcaggcaa cccagaagga agccggatga tcatccggct tttttacatc
    13141 atcagaaatg ccagtaactc attgccagcc ctaaaaacag taccagacca acatagttat
    13201 tattcataaa tgctttaaag caggcttcac gctcgcggtt ggcaatcagt ttttgttgat
    13261 aaacaaacag cgcgccagcc accagaattg accagtaata tccccagcct aagccattta
    13321 actcaccgat aatcgccatc agcgccagta cgccaatctg caaaatacca ataatcaatt
    13381 tatcgtattg gccgaacagg attgcggtgg atttaatgcc aatcttcaca tcatcatcac
    13441 ggtcaaccat cgcatactgc gtgtcgtatg ccaccgccca gagaatattg gcgaggaaca
    13501 ttaaccagca actcaatggc accgactcac tcacagcggc aaaagccatt ggaatcgacc
    13561 agccaaacgc cgcgcccagc accacttgcg gtagatgggt ataccgcttc ataaacgggt
    13621 atacccacgc cagcgccagc gcggcaatcg acaacagaat agtcatcgta ttcagcgtca
    13681 gcaccagtaa aaatgaaatc agtaccagca ctacaaacag cgcgcgcgcc tctttctccg
    13741 ttaccgcgcc gctgggaagt ggtcggttcg ccgtgcgctt aacatgacca tcaaacttgc
    13801 ggtcagcata atcattcacc acgcaaccag cagcgcgcat cagccagacg cccgcgacaa
    13861 acaccgccag gatccagagc tggggaacgc ccggtgtcgc cacccacaac gcccataatg
    13921 ttggccagag cagcagtaac gcgccaattg gcttatccgt acgcattaag cgatgaaacg
    13981 ccagcagctt attctgcgtc agactccact ccatattttt ttcctcttag tacaacggtg
    14041 acgccggtaa aaacagttcg gttagcaaca gcggtttacc gcttaatcgc aggcgggaac
    14101 gtcgccccca cagtccggca tcacggccta tctcaataaa gtcccgggtt aatgtcgatg
    14161 atgtgaacag atagcgtcct aacggcgttt tacccaattt ttgtaacgcc agctccggcc
    14221 cgcttaacgt tgacacagga acgacggtac gaccggcaag ccacggttca ccatcggcac
    14281 ataacaaaat ttcacgtaac cagtaacgag actctttcgg cagcagcggc agttcttcgg
    14341 gaatttcatt ctgctcgaca aacccttcgc ggatcatcgt cacgctcacc gtttttccct
    14401 gctgttcaaa acgttttgtc atggaatctt ccagcaacag ccagtcgagc agttgcggct
    14461 ccagggcagg gatctctgta aaatagcgca gcgcacgcag ttgcgttaac gcggggtgtg
    14521 acatgccgaa ctctccgtta cataacgtaa aggcattgta tcgcagaaaa atatttacgg
    14581 gagatgtaaa gcaaattaag tgaactaatg cgcaacaaaa acgcaacaat agtacaggag
    14641 gagaaaaaaa ggtgcgccag gagacgcacc agttgttgca aaatcagcac tgtggggcgt
    14701 aattacccct tgccttttac actgctgata aaggtggaac gggcagatgt tgaccccagg
    14761 cgttcagctt catcaagcag ttttaacgcc ttatcaacat cacctttcgc gacagcgttt
    14821 ttgatcgcgg tattaaaata actttccgtg tcgttgagca tcggctcgct tttcttcacc
    14881 ggtgccggag cgggtgcagc cacagctggt gccgccgtgt tgcctaccgt aaccggagct
    14941 ggagcggaag aaccaaatag aggcccgacc aacacgctgg aactggagtt cgttttgact
    15001 ttcagtttca gtaagccatc ggtggtatga cgagcaaccg ggtcaggaat atccgggatc
    15061 gagttaccga cgcctttggc ataggcttta gccgggtcga gcagttgagt cgtctgctgg
    15121 agatcttttt ccgtggtaaa gaccagaaca taaagttttt gctgccccaa cgccggtgtc
    15181 aggcgcataa cgccttccag ccgatctgca ctcatcacgc cgggttcctg gtaggtgaaa
    15241 taactgctgg ggaagaaggc tgatggggtc atgttctgat caagaatcag cacattcggc
    15301 gcaaatacgc tggtttgttt gttcacttcg ctggtcagcg tcagtgtcag ttcaccaatg
    15361 tttgccggga cgctgtacgc agcaaccgga ccactgatgc cagggacatt cagttgttgg
    15421 ccgccggtcg acagttgagt agtctgggtt ttagattgat cgaccggtgt ccaggtgagt
    15481 tgttgcagcg cggcagatgg aatggctggc gcgtcgctgg tgttttgcgg tacgtagtta
    15541 acatcggcaa ggctgattcc aggcgcgctt gccagcaacc ctgctgataa acagagggcg
    15601 atgagacttt tattcatttt cattgttttc acctcaaaat ctggagctca gcggtagcca
    15661 ggcaatagcg cgctaaaccc gataatcaga ggggctttcg ccccttaaag ctgtttttat
    15721 gccggatgcg gcgcaagcac cttatccggc ccaggttttg ctattaccac cagatttcca
    15781 tctgggcacc gaaggtccac tcgtcgctgt cgccacgacc gaagctaccg ccgttgaaat
    15841 cagccgacac agccttgccg tagtatgggt tggttgaaga gctaccggtg tagtcataac
    15901 cccatttctc atcccacttg gcgtaggttg cgaagacacg aatagccggg cgtgaccaga
    15961 tgctgtcgcc agcctgccat tgttgtgcaa gggtaatttt gtactgattg ttcttgtcgc
    16021 cggtgcgctg ggattcgacg ttgtcgtagc cgatttccat cacggtgctc atgattggcg
    16081 tccacttgta catcgggcga ataccgacgg tccaccactt ggtgccgttg tcgttatccc
    16141 agttgatatc ctggtacata cccacgtaca tcatgtccca gttgtcgccc atggagatcg
    16201 caccgtggtc gaggatacgc agcatgtgac cgttgttgtt gatattgtag gcaaattttt
    16261 cgttatcaaa cgcgacgcca gaaccctgcg acagaccttt accctgcgag gtcatcgagt
    16321 cagtagcgta ctgaacaaca aacttgttaa agcccttcag gacactctga gtatgttcag
    16381 cagtgaataa ccagccgtct ttcgatgcgc catcaaccag acgatagtta tcacgcaggt
    16441 tggcacgacc gtagtcgaca cccagttcta atgtgccgcc cgggttgatt tccatctgcg
    16501 ctaaacgcac atcgaaaacg tcgttcgcgg tttcgttggt atagtcataa atattgttgc
    16561 tggcgaaaga ggaagaacca cccgcttcag aggagcgggt tgctgccaga gagagtttac
    16621 cgaagccaac atcgatgttt tccagaccgg caccaggacc agaaatatcc cagtagtaga
    16681 agtcgatcat atgaacgtca tgacgttggt agaagcgctt acctgcccag atggtggagc
    16741 ctggtagcca ttcgatcagg tttttaccct gcacgtttgc ttcacggaag gccggatcgg
    16801 tagcttccca gtcattctgt tgcgcgacgg aataggccac gttagtgtcg aaatagaagc
    16861 tcttatcgcc ctctttccac acttcctgac ccaattttaa ttcagcataa gtttcacatt
    16921 cgttgccaag acggtattta ctttgagcac cggtagtctg gaaacactgt tgttcgccgc
    16981 cgctacctgt ccagccaata ccggaacgtg catagccgtg gaaatcaaca gccattgcct
    17041 gagcagacat tacgcccgct gcgacggcaa ccgccagagg aagtttgcgc agagtaatca
    17101 tcattctatc tcctgagtca ttgcttttct tttttcacat cacctgtgac aggctttgtg
    17161 tgttttgtgg ggtgcttaaa cgcccggctc cttatgcagt cgacgacatg cagtgccatc
    17221 ctcacggaac agatggcaac gctctggcgg caggccgata gcgaatgtgg caccttcttc
    17281 taccaacacc acgtcgttct ggcggtacac caggttttga cgaatggaag ggatctggat
    17341 atggatttgc gtttcgttgc cgagttgctc gacgacctga acttcaccct caaggatgac
    17401 gtcagcgata tcactcggca gtagatgttc cgggcgaata cccagtgaca tattggctcc
    17461 aacctggaca tcacggcttt cgaccggtag ccagacttgc tgacgattcg gcatcggcag
    17521 ctccacctgc acttgatcga ttgcggtggc ggtcactttt actggcagga agttcatctt
    17581 tggagaaccg ataaatccgg cgacaaaacg gtctgccgga tagtgataca gctccagcgg
    17641 tttcccaacc tgcgccacgc gaccagcgtc cagcaccacg attttgtcgg ccagcgtcat
    17701 cgcttcgacc tgatcgtggg tgacgtaaat cattgtgcgg cccaggcgtt tatgcagacg
    17761 ggagatttcg atacgcattt gcacacgcag tgcagcatcg aggttggaga gcggttcatc
    17821 gagcaaaaat acgcttggct cggccaccag cgtacggcca atcgccacac gctgacgctg
    17881 accaccggag agcgctttcg gtttgcgatc cagcaaatgc gccagttgca gcacttccgc
    17941 aacctggtta actcgctggt taatcacttc ttttttcgcg ccagccagtt tcaggccaaa
    18001 tgacatgttt tctgctactg acaggtgggg atagagcgcg taagactgaa acaccatacc
    18061 aacgccgcgt tctgcaggcg gagtgtcatt catccgtttc tcaccgatga acaggtcgcc
    18121 gctggtgatc gtctcaagcc cggcaatcat gcgcagtaaa gtcgatttac cgcagccaga
    18181 cggtccgaca aacaccacga attcaccttc atggatatcg agattgatat ctttcgatac
    18241 cacgacctcg ccccaggctt tcgttacatt ttgcagctgt acgctcgcca tgcccttctc
    18301 cctttgtaac aacctgtcat cgacagcaac attcatgatg ggctgactat gcgtcatcag
    18361 gagatggctt aaatcctcca ccccctggct tttttatggg ggaggaggcg ggaggatgag
    18421 aacgcagctt ctgtgaacta aaccgaggtc atgtaaggaa tttcgtgatg ttgcttgcaa
    18481 aaatcatggc gattttatgt gcgcatctcc acattaccgc caattctgta acagagatca
    18541 cacaaagcaa cgatggggcg taggggcaag gaggatggaa agaggttgcc gtataaagaa
    18601 actagagtcc gtttaggtgt tttcacgagc acttcaccaa caaggaccat agattatgaa
    18661 aataaaaaca ggtgcacgca tcctcgcatt atccgcatta acgacgatga tgttttccgc
    18721 ctcggctctc gccaaaatcg aagaaggtaa actggtaatc tggattaacg gcgataaagg
    18781 ctataacggt ctcgctgaag tcggtaagaa attcgagaaa gataccggaa ttaaagtcac
    18841 cgttgagcat ccggataaac tggaagagaa attccctcag gttgcggcaa ctggcgatgg
    18901 ccctgacatt atcttctggg cgcacgaccg ctttggcggc tacgctcaat ctggcctgtt
    18961 ggctgaaatc accccggaca aagcgttcca ggacaagctg tatccgttta cctgggatgc
    19021 cgtacgttac aacggcaagc tgattgctta cccgatcgct gtcgaagcgt tatcactgat
    19081 ttataacaaa gatctgctac cgaacccgcc aaaaacctgg gaagagatcc cggcgctgga
    19141 taaagaactg aaagcgaaag gtaagagcgc gctgatgttc aacctgcaag aaccatactt
    19201 cacctggccg ctaattgcgg ctgacggggg ttatgcgttc aagtatgaaa acggcaagta
    19261 cgacattaaa gacgtaggcg tggataacgc tggcgcgaaa gcgggtctga ccttcctggt
    19321 tgatctgatt aaaaacaaac acatgaatgc agacaccgat tactccatcg cagaagctgc
    19381 ctttaataaa ggcgaaacag cgatgactat caacggcccg tgggcatggt ccaacatcga
    19441 caccagtaaa gtgaattatg gtgtaacggt actgccgacc ttcaaaggtc aaccatctaa
    19501 accgttcgtt ggcgtgctga gcgcaggtat taacgccgcc agtccgaaca aagagctggc
    19561 gaaagagttc ctcgaaaact atctgctgac tgacgaaggt ctggaagcgg tcaataaaga
    19621 caaaccgctg ggtgcagtag cgctgaagtc ctatcaggat gaacttgcga aagatccacg
    19681 tattgccgcc accatggata acgctcagaa aggtgaaatt atgccgaaca tcccgcagat
    19741 gtccgctttc tggtatgccg tgcgtactgc ggtgatcaac gccgccagcg gtcgtcagac
    19801 tgtggatgct gcgctgaaag atgcacagac tcgtatcacc aagtaatgct gtgaaatgcc
    19861 ggatgcggcg taaacgcctt atccggccta cgaaattgaa acgtatgtag gcctgataag
    19921 acgcgtcagc gtcgcatcag gcagttgttg tcggataagg tatgaaagcc ttatccgtcc
    19981 tggaatgagg aagaacccca tggatgtcat taaaaagaaa cattggtggc aaagcgacgc
    20041 gctgaaatgg tcagtgttag gtctgctcgg cctgctggtg ggttaccttg ttgttttaat
    20101 gtacgcacaa ggggaatacc tgttcgccat taccacgctg atattgagct cagcggggct
    20161 gtatattttc gccaatcgta aagcctacgc ctggcgctat gtttacccgg gaatggctgg
    20221 aatgggatta ttcgtcctct tccctctggt ctgcaccatc gccattgcct tcaccaacta
    20281 cagcagcact aaccagctga cttttgaacg tgcgcaggaa gtgttgctag atcgctcctg
    20341 gcaagcaggc aaaatctata attttggtct ttatccggcg ggcgatgagt ggcaactggc
    20401 gctcagcgac ggcgaaaccg gcaaaaatta cctctccgac gcttttaaat ttggcggcga
    20461 gcaaaaactg caactgaaag aaacgaccgc ccagcccgaa ggcgaacgcg cgaatctgcg
    20521 cgtaattacc cagaatcgtc aggcactgag tgacattacc gccattctgc cggatggcaa
    20581 caaagtgatg atgagctccc tgcgccagtt ttctggcacg caaccgctct acacactcga
    20641 cggtgacggc acgttgacga ataatcagag cggcgtgaaa tatcgtccga ataaccaaat
    20701 tggcttttac cagtccatta ccgccgatgg caactggggg gatgaaaagc taagccccgg
    20761 ttacaccgtg accaccggct ggaaaaactt tacccgcgtc tttaccgacg aaggcattca
    20821 gaaaccgttc ctcgccattt tcgtctggac cgtggtgttc tcgctgatca ctgtcttttt
    20881 aacggtggcg gtcggcatgg ttctggcgtg tctggtgcag tgggaagcgt tgcgcggcaa
    20941 agcggtctat cgcgtcctgc ttattctgcc ctacgcggtg ccatcattca tttcaatctt
    21001 gattttcaaa gggttgttta accagagttt tggtgaaatc aacatgatgt tgagcgcgct
    21061 gtttggcgtg aagcccgcct ggttcagcga tccgaccacc gcccgcacga tgctgattat
    21121 cgtcaacacc tggctgggtt atccgtacat gatgatcctc tgcatgggct tgctgaaggc
    21181 gattccggac gatttgtatg aagcctcagc aatggatggc gcaggtccat tccagaactt
    21241 ctttaagatt acgctgccgc tgctgattaa accgctgacg ccgctgatga tcgccagctt
    21301 cgcctttaac tttaacaact tcgtgctgat tcaactgtta accaacggcg gcccggatcg
    21361 tcttggcacg accacgccag ccggttatac cgacctgctt gttaactaca cctaccgtat
    21421 cgcttttgaa ggcggcgggg gtcaggactt cggtctggcg gcagcaattg ccacgctgat
    21481 cttcctgctg gtgggtgcgc tggcgatagt gaacctgaaa gccacgcgaa tgaagtttga
    21541 ttaagggaga taacaaaaat ggcaatggtc caaccgaaat cgcaaaaaac acgtttgttt
    21601 attactcacc tgctactgct actttttatc gcggcgatta tgttcccgct actgatggtc
    21661 gtcgctatct cactgcgcca ggggaacttt gcgaccggca gcctgatccc ggagcaaatc
    21721 tcctgggatc actggaaact ggcattaggt tttagcgtgg aacaggctga tggtcgcgtt
    21781 acaccgccgc cgtttccggt actgctatgg ctgtggaact cggtaaaggt cgccgggatt
    21841 tccgcgattg gcattgtggc gctctccacc acctgcgcct acgctttcgc ccgtatgcgc
    21901 tttccaggca aagcgacgct gctgaaagga atgctgattt tccagatgtt cccggcagta
    21961 ctttcactgg tcgcgttgta tgcgttgttt gatcgtctgg gtgagtacat tccattcatt
    22021 ggcctgaata ctcacggcgg cgtaattttc gcgtatctgg gtgggattgc gctgcatgtc
    22081 tggaccatca aaggctattt cgaaaccatc gatagttcgc tggaagaagc ggctgcgctg
    22141 gatggtgcga caccgtggca ggccttccgc cttgtcctgt tgccgctgtc agtaccgatt
    22201 ctggcggtgg tgttcatcct gtcgtttatc gctgccatta ctgaagttcc ggtcgcatcg
    22261 ctgttactgc gtgacgtaaa cagctacacc ctggccgtgg ggatgcagca atacctcaac
    22321 ccgcaaaact acctgtgggg tgactttgcc gctgctgccg tgatgtctgc attaccgatc
    22381 accatcgtct tcttgctggc tcaacgctgg ctggtcaacg gcctgacggc aggtggtgtg
    22441 aaaggttaaa gatgttgttc tgccaatgtt atgccgctgc accctcaact tacgttatcc
    22501 caacttgtga ctgttattcg gcgctccacg gagcgccttt ttttactccc gcttcagccg
    22561 tttcgaatta cataaccaca gggtgatcac cagtaacagg atcgcagccg agtagatcaa
    22621 cacatccagt ggcgatttat gatcgacgat gatcaagcgc acaatggcgg tgatcccaat
    22681 atagacaaag taacgtagcg ggaagtgaaa accggactga aagtacttca caatcaacgc
    22741 gataaattcg aaatagagaa agtaaaccac cagcccttct accagctcat atttgctggt
    22801 ttgttctggc gcgaacagca catcagccag atgcaccgtt tctttgccga ggaagacaac
    22861 caaaatcagg ccgaggcaca gcaggccaag attgagtacg gtctgcaaaa tggtggagat
    22921 aaactccacg cgcggacgag agagtgacgt catagcagta cctccttcac agtggcgagc
    22981 ttcctgtata acgcaaaaat gtgacgggga tctatatttt ttgtgtatgt tttgttcaac
    23041 ctggattaag tgggcatctc ccatcaacat cgtatctggc aaaacacact gtccaaaaaa
    23101 tcacccattc aggtgattcc cagccaccag caatgcgcga aaaatcgaca atgttcctct
    23161 ggaacaaatg aaacgtaacc tgataaaggt ggtaaaaatg aagccttatt ttgctgcttt
    23221 gatgttatca gtctctgttc tgcttatgct ggtccgctcg gtactgctga taaagccgac
    23281 ctaccgcaaa gcaatgtctc cagcccaatg atggcccagt cgctcaggca accagacctg
    23341 caaccgatat cgacggacag gaagacagaa tgttttcgcc tttacacacc agatcgcaaa
    23401 ccgggggtga actgcgttcc tgatggctcg acaggccatt aagaatgagg gtgccagtga
    23461 gtgcgaaaac tggcaccttt ttcatgactt agcggaaatt gacgctacgg tcgctggtca
    23521 tgtcatacaa ctggaactta cgcccaagtt gctgaccacc gtcacgcgtc agcggcgtcc
    23581 agccaattgc cgcacggctg cgtgtcgggc cagacgagaa gagatccagc ggtacagaga
    23641 catacacgcc tttggtgaag tccccttcgc cgtactcctc ttttgaaaca ttagtgatcg
    23701 tggcatagcc acccaccacc acgccgctat caaagcgttt ggcgatctcc agcgtgccgc
    23761 ctttatcccc tgccagatac tgcccgacgc tcgctttcac taacacatcc tgagcgaaag
    23821 atggcgtcca gtaggcggtc agatgtccgg ttttcacgct gtagtcggtg aatttcatca
    23881 tatcttttgc actacgccag tcgcgctgtt taacgtagtt ggcatccaca ccaaacgccc
    23941 agttgctgtc cagcgggcga tacaacactt ctgcccccgc accgccaaac atggtttcga
    24001 gataacctcc gtagacctga ccgtagaagc cgttgcccag atgctggaag tagttggctt
    24061 gcaggttatt cacatagaca tcgttctgca catactcgcg cacatgggta cgaacgcgcg
    24121 gtaagtgcga gtcctgcggc ggattggtgt agttaaactt gtcgtagttg ttggcaaggt
    24181 tagcaaacag gctgccggtg gtcagcagat ggtccgtcag ccacaaatcc gccgttccca
    24241 tcacgcccag ctgatacatg taaaagtttt ccgggccacc gaccgactgg ttcagcaccg
    24301 gatcgatatg gaaatcaaag cgtgatttgt cgatatacca gccctgctcg gtggactgcg
    24361 gaaccactgg ctcgacgcgt ttttgcgcca gcttcgtttc gtggcccaac ggctctccgg
    24421 cgagatgatt tttcaggctg gcgacatcgg tttccgtcgt cacctgcggc atgttaaggc
    24481 gattttccgt aatgcggatc gttttgatcc catccggcag atcgttcatc acgatccgat
    24541 tagcacggat gatcccttca cgcgaatcac gatatttcac ctgctcgccg gtgacataca
    24601 gcgtatcgcc tttcgtctgg atctgcggat cggccagtcc ggcgttgtat ttcaacagcg
    24661 ttaactgatt cgccaccacc gaatgctgca aaatggcatc ctgtggttgc ggttgatatt
    24721 gcgggcgggc gttatcgttg taggacgggc gcagatcgtt aaagttggtg cgcagcgtga
    24781 cgccaaacat aaaagtgttg ccacgttcat agctaaggtt aacgtcggcc caatcggtaa
    24841 cgcgataaat cgccccgacg ttaaacttac ttttttgctc cagcttaccg gcaaaatcct
    24901 gctgataatt attgccttca tactccagtt tcaggcgcag cggttgccag ggcgtctggt
    24961 attccacgcc gccaaacagt gaggccggac cgtggaacat ctggctgccg tcgatagatc
    25021 ccgcttgttt gtagctgtta tcgcgatagc aatatttgtc actggccgaa cagagcggat
    25081 ttttcacatt accgctggtg cccagatatc cccagcccag gccgagcgta aaatcgaatg
    25141 gcccccaggc tttgctggcg acaagatatt ccgcatcgaa cagccccgta ccgccgatat
    25201 cccgcgcacc aaccgccact tgcggcagcc agtaactctc ttcccacaaa cgcagtttga
    25261 gatcgaaggc tttatcttta tacgtttgat cgccagagaa cgcttcgacg ctgctgtact
    25321 gccgggtgcg cacgtcggtg tagcgcagcg ttgtttccag ccacgggaag agttgcactg
    25381 aagctgagta ataacggtac tgatcgttat cgcgatagtt cagactcaac tccccttccc
    25441 gtgccatacg cgcggtgggc gtttgtaata atcctacgcc accgaaatcc gactgcgacg
    25501 gaccaatggg cgcaggatat gtttcgccgt agcaagctgt gctaatgccc agcgccagta
    25561 agctaagcag atgtcttttt ttcattattg aggtatccgc tgcgtcagag tctgaagaat
    25621 gtcggcgtta agggcatcag gcgtctcact ccagacggag tccgcgaggc caacataaat
    25681 aatgctgccg ggcatcggct ctacgtgacg cttgttccag taagccaccg gcgctttttg
    25741 cgtgcgtccg tccgggtaaa ccacccacgc gtagctgcga tccgcaccgc taagcaggtt
    25801 ctgcccggag agatagctcg ccacgtcgcg acctggaatg aatggctgct tgccaggacg
    25861 gctgataagc ccgaataacg tgaccgtgga cggcggtggt ccgacccaca gcgtgtcgtt
    25921 gccctgcaac ggcgggttac cgcgttcggc aacgcggacg atatcaggat caagattgat
    25981 tttttgcctg cccgtcactt tcaacgcctg aatttgctgg cgtaaggcgt taatcgcggc
    26041 ggcatcgtcg gcgctggaat ctgcagccag ttctgccagc cgtgtcagca acgcctgctg
    26101 ctgacgcaat gctgccgccg ttgccagctc ttcgctaatc accgcaccgg gccaccaact
    26161 gtttgccagc cgtggttggc ctaccagatc gattaaatgc tctgcacccg tcagcgtttt
    26221 ggcctcactg ctgccattgc tgaatacctt gacggttccc gccgcgaaga ctgatgacgc
    26281 ccccacactc aaaatcaatg cgacaatagt ttgtttaatc attgttttgc cgccttgatg
    26341 agagtggttt tcaccgggaa ataatccgcg ccgagatact gttgcgactg gcgaatttgc
    26401 ccttcgctgt cgatccagta gcggttatgc cagcgcgcct ggtcggtgga gacttcttcg
    26461 tccagcacgc gaaccggggt ttcatcgctg ccgaccttga cggtatcggt gccatcccat
    26521 ttgaagactg agcgtgcggt ggcgtagcgt acctgctggt actcggtcca gcccatcgtg
    26581 cgcgtccatg ttgcaccatc aacaatttgt gcgggtttaa tcagcgggtc ggcggcaagg
    26641 ttattcactt caatcaggtt gtcgccgcca agcaaagtct tcaccagacg accatgttgt
    26701 gtgacgagag tggcctgatc ctgagtgacc catttttgtt gtccgtcttc agcgaaggcc
    26761 agcaccacaa ataactgcgg cccgccatta agctgcatgt actggctggc gtagggcata
    26821 ttttgaatat catcgtccgt cagctgtacg cctggcgtgc cgaacagact gtcccacagt
    26881 gaattgccca gctctttagt cgtggctgaa caggcctgta atagcaggca gataagaatg
    26941 agtgcaggtc gcttcacgac tcttctccag aggggcagaa taaccacacc ggagtgtggt
    27001 tatggttaat ccccttcata cttcaagctg cagatgcggt gaccgcactc gttcaccccg
    27061 gtcacatagt tatctatgct cccggggact cactcgcttg tcgccttcct gcaactcgaa
    27121 ttattttggg gatacatact ttattactgg gtactggtgg tcgtggttgt tgtggtcccg
    27181 gtattagaac catcaccgcc accgcttgcc gcgagcgcga cacccaccgc cgagcttacg
    27241 gtgctgacgc tggtcgcgga ggaactcccc gccgaaaccg acgttgctgc cgaccctgcc
    27301 gcttcgccca cctgtacagg tgcagcccac acagaagtcg ccgcaagcgc agatatggca
    27361 aaaatgccat acagaacttt tttcataaca atttcccttc attgaatgaa tggagattta
    27421 cccaaaaaca tttcgggcgg gatcgagtat acacaactaa agcatgcgaa ttacggcgcg
    27481 gggaattagt gatggggttt taggataatt ggaatgaata tcaaatataa gcttcagctt
    27541 atatataaat agcactaaaa atcataagct taaaataaaa ataaaagtaa ttaatctgcg
    27601 tattttccga aatcaatcac gattaaaaat ctattttagg attaacctca cggtatgatt
    27661 tccgttaaat cacagacaag gggatttatc tgataaaaaa tgccgacata acgtcggcat
    27721 tgtttttaag gctatttaat caatcgtact atgtattaat tgccgaatac gatgtacata
    27781 tcggcatcga cctgtaggcc tgataagacg cgaccgcgtc gcatcaggca tcggttgccg
    27841 gatgcggcgt gaacgcctta tccggcctac atatcgacaa tgattaaccg cgccacgctt
    27901 tatagcggtt aatcagacca ttggtcgagc tatcgtggct gctgatttct ttgtcatctt
    27961 tcagctctgg cagaatacgg ttcgccagtt gtttacccag ctccacgccc cactggtcga
    28021 aggtgaagat gttcaggatc acgccctgag taaagatttt gtgctcatac agcgcaatca
    28081 acgcacccag gctgaacgga gtgatttcac gcagcaggat ggagttggtc gggcggttac
    28141 cttcgaacac tttgaacggc accacgtagt caagcgttgc cggatcttta ccctgatcac
    28201 gatattcctg ctcaaccact tcgcgggatt taccaaacgc cagcgcttcg gtctgagcaa
    28261 agaagttaga cagcagtttc tggtgatgat cagagagcgg gttatgggtg atagccggag
    28321 cgatgaaatc gcacggtacc attttggttc cctggtggat cagctggtag aacgcgtgct
    28381 gaccgttagt acctggttca ccccagataa tcgggccagt ctggtaatcc acaacgttac
    28441 cgttacggtc aacatacttt ccgttggact ccatattgcc ctgctggaag tacgccgcga
    28501 aacggtgcat atactggtca tacggcagaa tcgcttcagt ttccgcacca aagaaattgt
    28561 tgtaccagat gccgatcagc gccagcaaga caggcaggtt tttctcggca ggcgtggtgg
    28621 agaaatgctt gtccatcgcg tgtgcgccag aaagcagttc aacaaaatta tcaaagccga
    28681 tggagagaac aatcgacagg ccaattgctg accacaaaga gtaacggccg ccaacccagt
    28741 cccagaactc gaacatgttg gcagtatcaa taccaaactc gccaacggct ttggcattgg
    28801 tggaaagcgc cgcaaagtgt ttcgcaacgt gtttttcatc accagccgct ttcaggaacc
    28861 agtcacgcgc actatgggcg ttggtcatgg tttcctgagt ggtgaaggtt ttagatgcta
    28921 ccaggaacag cgtggtttcc gggtttactt ttttcagcac ttccgcgatg tgagtcccat
    28981 cgacgttaga aacaaagtgc atgttcaggt ggtttttgta tggacgcagt gcttcagtca
    29041 ccatgtacgg gccgaggtca gaaccgccga tcccgatgtt cactacgtca gtgattgctt
    29101 tgccggtata gcctttccac tcaccggaga taatcgcttc cgagaaggtt ttcatcttct
    29161 ccagcaccgc gttgacttcc ggcataacat ctttgccatc aaccagaatc ggggtattgc
    29221 tacggttacg cagcgctacg tgcagtacag cgcggttttc ggtgcggttg atcttctcac
    29281 cagagaacat cgacttaatc gcgcccgcca gatcgcactc tttcgccaga tcttgtaatt
    29341 tcgccagcgt ctcttcagtg atgcggtttt tggagtaatc caccagcatc tgatcgtcga
    29401 aggttgcgga gaacttagaa aaacgatcgc cgtctttagc aaaaagatcg gcgatcgtaa
    29461 cgtctttcat ttcatcgaag tgtttctgta gtgcctgcca ggcagcggtc tgcgttggat
    29521 tgatgttttt cattagcaat actcttctga ttttgagaat tgtgactttg gaagattgta
    29581 gcgccagtca cagaaaaatg tgatggtttt agtgccgtta gcgtaatgtt gagtgtaaac
    29641 ccttagcgca gcgaagcatt tattagctga actactgacc gccaggagtg gatgaaaaat
    29701 ccgcatgacc ccatcgttga caacggcccc gctcaccctt tatttataaa tgtactacct
    29761 gcgctagcgc aggccagaag aggcgcgttg cccaagtaac ggtgttggag gagccagtcc
    29821 tgtgataaca cctgaggggg tgcatcgccg aggtgattga acggctggcc acgttcatca
    29881 tcggctacag gggctgaatc ccctgggttg tcaccagaag cgttcgcagt cgggcgtttc
    29941 gcaagtggtg gagcacttct gggtgaaaat agtagcgaag tatcgctctg cgcccacccg
    30001 tcttccgctc ttcccttgtg ccaaggctga aaatggatcc cctgacacga ggtagttatg
    30061 tctgaaattg ttgtctccaa atttggcggt accagcgtag ctgattttga cgccatgaac
    30121 cgcagcgctg atattgtgct ttctgatgcc aacgtgcgtt tagttgtcct ctcggcttct
    30181 gctggtatca ctaatctgct ggtcgcttta gctgaaggac tggaacctgg cgagcgattc
    30241 gaaaaactcg acgctattcg caacatccag tttgccattc tggaacgtct gcgttacccg
    30301 aacgttatcc gtgaagagat tgaacgtctg ctggagaaca ttactgttct ggcagaagcg
    30361 gcggcgctgg caacgtctcc cgcgctgaca gatgaactgg tcagccacgg cgagctgatg
    30421 tcgaccctgc tgtttgtcga gatcctgcgc gaacgcgatg ttcaggcaca gtggtttgat
    30481 gtacgtaaag tgatgcgtac caacgaccga tttggtcgtg cagagccaga tgtagccgcg
    30541 ctggcggaac tggccgcgct gcagttgctc ccacgcctca atgaaggctt agtgatcacc
    30601 cagggattta tcggtagcga aaataaaggt cgtacaacga cgcttggccg tggaggcagc
    30661 gattatacgg cagccttgct ggcggaggct ttacacgcat ctcgtgttga tatctggacc
    30721 gacgtcccgg gcatctacac caccgatcca cgcgtggttt ccgcagcaaa acgcattgat
    30781 gaaatcgcgt ttgccgaagc ggcagagatg gcaacttttg gtgcaaaagt actgcatccg
    30841 gcaacgttgc tacccgcagt acgtagcgat atcccggtct ttgtcggctc cagcaaagac
    30901 ccacgcgcag gtggtacgct ggtgtgcaat aaaactgaaa atccgccgct attccgcgcg
    30961 ctggcgcttc gtcgcaatca gactctgctc actttgcaca gcctgaatat gctgcattct
    31021 cgcggtttcc tcgcggaagt tttcggcata ctcgcgcggc ataatatttc ggtagactta
    31081 atcaccacgt cagaagtgag cgtggcatta acccttgata ccaccggttc aacctccact
    31141 gacgatacct tgctgacgca atctctgctg atggagctgt ctgcactatg ccgggtagaa
    31201 gtggaggaag gtctggcgct ggtcgcgttg attggcaatg acctgtcaaa agcctgcggc
    31261 gttggcaaag aggtattcgg cgtactggaa ccgttcaaca ttcgcatgat ttgttacggc
    31321 gcatccagcc ataacctgtg cttcctggtg cccggcgaag atgccgagca ggtggtgcaa
    31381 aaactgcatt ttaatttgtt tgagtaaaca cagtatggcc cggaagacat tatttcgggc
    31441 cgtattgttt ttcttgtcat tatgctcatc aataaacgag cctgtactct gttaaccaac
    31501 gtctttatcg gagaataatt gcctttaatt tttttatctg catctctaac taattagaga
    31561 aagagataaa tagttaagag aaggcaaaat gaatattatc agttctgctc gcaaacgaat
    31621 tcccgtacac tgaactctct ggctcactga gggcacaatc atggcactcc cccgtattac
    31681 ccaaaaagag atgactgaac gcgagcagcg tgaactaaaa acactgctgg atcgggcgcg
    31741 gattgcgcat ggtcgcgtgt tgactaattc ggaaaccaac agcattaaaa aagagtacat
    31801 cgataagctg atggttgagc gtgaagcagc agcaaaaaaa gcccgccaat tgaagaaaaa
    31861 gcaggcttat aaaccggatc ctgaagcgtc atttagctgg tcagcaaata cgtcgacgcg
    31921 cggcaggcgc tgattaacgc cccttctttt tacgccccgg cgaggtaaaa cgcttaccgt
    31981 tggacgccgg gcgaccgcct ttttccgccg ttttttccat cttaacgact ggacgtttga
    32041 tgcccgccgt tttcggtttg gccttcgttt taggttttgc ctcggaagag gaattttcga
    32101 tgagcttaaa gaggtcgatt aactcatcgt cagttaaatc gcgccactca cccagcggaa
    32161 tgccgcttaa gctgacgttc ataatgcgcg tacgttccag ctttttcact tcgtaaccaa
    32221 agtgctcgca catgcgccgg atctgacggt tcagcccctg caccagggta atgcggaaga
    32281 caaacggcgc ttcttttttg actttgcact ttttcgtcac cgtcccgagg atcggcaccc
    32341 ccgcccccat accacgaata aattcatcgg taatcggttt atcgaccgtc accagatact
    32401 ctttctcatg atcgttaccc gcacggagga tcttattcac cagatcgccg tgattggtga
    32461 ggaaaatcag cccctgggaa tctttatcca ggcggccaat cgggaacacg cgtttgctgt
    32521 ggttaacgaa atcgacaatg ttatcgcgct cgccatcttc ggtggtgctg acaataccga
    32581 cgggcttgtt cagggcgata agtaccaaat cttcggcttc ccgaggctca atcaactgac
    32641 cgtttacttt cacaacgtcg ccgggtttca cctgatcgcc aatggtggct cgcttgccat
    32701 taaggaacac attgccttgc tcgatatagc gatccgcttc gcggcgtgag caaattccgc
    32761 tttcgctgat gtatttattt aaacggactg atgagtcggg cagcataaat tctcctgtaa
    32821 aacgcggaat ataccgtacc tcttagcggg gaaaaacagg gttatgcggc aacgtcgaaa
    32881 ttaaatgtga tctgccacgc cttttatcac aaaagtgtct ccggaagtac gatgcaaatt
    32941 tgcctggcaa aataagaata atcaacaaat tacgcatcgc tgcacacctg tgctgatgac
    33001 tactacgcag agaaacaaat gtgcaagaga aagcgctatt tccccccatg caaagagtga
    33061 aaaatcgctt tgaaaggcca tcatgatggg gttatatcag atcaagcggt gagaaagcgt
    33121 atttgcacaa atgtgcagaa ccaggaggcg gcaatggaaa acagtgacga tatccgtttg
    33181 attgtgaaga ttgcccaact ctattacgaa caggatatga cgcaggcgca aattgcgcgc
    33241 gaactgggta tttaccgcac caccatcagc cgcttgctta aacgaggccg cgatcaggga
    33301 attgtcacca tcgccatcaa ctatgactac aacgaaaatc tctggctgga gcagcaagtg
    33361 aagcaaaagt ttagcctgaa agacgttgtg gtggtgtcag gaaatgatga ggatgaagat
    33421 actcaactgg cgatgatggg gttacacggc gcgcaactgc tggatcgctt gctggaacct
    33481 ggcgatattg tcggtttttc ctggggccgc gcggtgagcg cactggttga aaacttgccg
    33541 caggcggggc aatcgcggca gttaatttgc gtaccaatta ttggcggacc gtccggtaaa
    33601 ctcgaaagcc gctatcacgt aaacacatta acctacagcg cggcagcgaa gctgaaaggg
    33661 gaatcgcatc tcgcggattt tccggctctg ctggataacc cattaattcg taatgggatc
    33721 atgcagtctc agcactttaa aaccatctct gcctactggg ataatctgga tgtcgccctg
    33781 gtgggaattg gctcaccggc cattcgcgac ggcgctaact ggcatgcgtt ttatggtggt
    33841 gaagagagtg acgacctgaa tgcccgccag gttgctggcg atatttgctc gcgctttttt
    33901 gatattcacg gcgcaatggt tgaaacgaat atgagcgaaa aaacactctc tatcgaaatg
    33961 aataaattaa agcaggcgcg gtattccatt ggcattgcca tgagtgaaga aaaatacagc
    34021 ggaattattg gtacactgcg tggaaaatat attaattgtc tggtaacgaa tagcagcaca
    34081 gctgaactat tactgaaata aaaatcttcc cgatgtttat cgtaataaaa attacggcgg
    34141 gtccctatta tctaaaatca ggagtctgtt atgcaaacgt ggttaaattt gcaggataaa
    34201 atcattatcg tcaccggcgg cgcatgcggt attggtctgg cgattgtaga ggaattatta
    34261 gcacaaggcg cgaatgtaca gatggtcgat attcacggtg gcgatggaca atatgaaggt
    34321 cataaaggct atcagttttg gccgaccgat atttccagcg ccaaagaggt aaatcatacg
    34381 gtagcggaaa ttatccagcg ttttggtcgc atcgacggtc tggtcaataa cgccggggtc
    34441 aatttcccgc gtctgctggt cgatgagaaa gcgcctgccg ggcagtatga actcaacgaa
    34501 gctgcattcg aaaaaatggt caatatcaac cagaaaggcg tttttctgat gtcgcaggcg
    34561 gtggcgcgac agatggtcaa acaacatgat ggcgtgattg tgaatgtttc ctcagaaagt
    34621 ggactggaag gctcagaagg ccaaagctgt tacgccgcta ccaaagccgc gctcaatagc
    34681 ttcacgcgct cctggtcgaa agagctgggt aaacacggca tccgtgtggt cggtatcgcg
    34741 cccgggattc tggaaaaaac cgggctacgc acgccggaat atgaagaggc gctggcgtgg
    34801 acgcgcaata tcaccgtcga gcagctgcgt gaaggctata ccaaaaacgc cattcctatt
    34861 gggcgcgccg gaagattagc agaagtcgct gattttgttt gttatctgct gtctcaacgc
    34921 gccagctata tcaccggagt aaccactaac attgcgggcg gcaaaacgcg cggctaagga
    34981 ggctttatgg tcaacgccat tttttgtgcc cacggcaaat tagcttgcgc catgctggaa
    35041 tcggttcaga tggtttacgg caacgccaat gttgaagccg ttgaatttgt gcccggtgaa
    35101 aacgccggag acatcgtagc aaagctggaa aagttagtaa gcattcacaa tcaagatgag
    35161 tggctgattg ccgtggattt acagtgcggc agcccgtgga atgcggcagc gatgcttgcg
    35221 atgcgcaatc ctcgtctgcg ggtaatcagc ggcctttctc tgccgctggc gctcgaactg
    35281 gttgataacc agcacagcat gaatgttgat gaactgtgtg aacacctgac gcaaatcgca
    35341 aagcaaacct gcgttgtctg gaaacaactg gcaacggcag aggaggattt ctgatgaaca
    35401 ttacgctcgc ccgcattgat gaccgtctga tccacggcca ggtcactacc gtctggtcga
    35461 aagtggctaa tgcccagcac attattatct gcaatgacga agtttataac gatgaagtgc
    35521 gccggacatt attgcgtcag gctgcgccac cgggaatgaa ggttaacgtg gtcaatattg
    35581 aaaaagccgt cgcggtttat cacaacccgc aatatcagga cgaaaccgtt ttttatcttt
    35641 tcactcgccc acaggatgcc ttagcgatgg ttcgccaggg ggtaaaaata gacaccctga
    35701 atattggcgg catggcctgg cggcccggta aaaaacagtt aaccaaagcg gtttcattag
    35761 atgatgatga tattaacgca tttcatgagt taaataatct tggcgtcata ctggatttac
    35821 gcgtcgtagc gtcagatccc tcaatcaata ttatcgacaa aataaacgaa cagttaatcg
    35881 caaattaaat aatagcgcct gtgtacatat gctttaacag gcagggatga cgacacttaa
    35941 aaggtgacaa atcatggaaa taagtaccct acaaatcatt gctatatttc ttttttcctg
    36001 tattgccgga atgggcagcg tgctggatga atttcagact catcgtccgt taattgcctg
    36061 tacggtgatt ggtttaattc tcggtgattt aaaaaccgga attatgctcg gtggtacgct
    36121 ggaattgata gctctcggct ggatgaacgt cggcgcggcg caatctccgg attctgcact
    36181 cgccagcata atctccgcca ttctggttat cgttggtcag caaagcatcg ccaccggaat
    36241 cgccatcgcg ttgcctgtgg ctgcggcagg ccaggtgctg acggtgtttg cccgtaccat
    36301 caccgtagtg ttccagcacg cggcggataa agcagcggaa gaggcgcggt ttcgcactct
    36361 cgatattctg catgtctccg cgcttggcgt gcaggcgctg cgcgttgcta ttccggcact
    36421 aattgtctcg ctgttcgtca gcgctgatat ggtgagcaat atgctgagcg ccattcccga
    36481 atttgtgacc cgtggactgc agattgctgg cggttttatc gtggtggtcg gttacgccat
    36541 ggtgcttcgc atgatgggcg tgaaatattt gatgcctttc tttttcctcg gtttcctcgc
    36601 aggtggctac ctcgatctca gtctgctggc gttcggtggc gtcggcgtga tcatggccct
    36661 gctctacatc cagttaaatc cacagtggcg taaagctgaa ccacatcccc agaccaccac
    36721 tatcaccgcc cttgaccaac ttgatgatta acggagccat catggaacag agaaaaatta
    36781 cacgcagcga tctggtgagc atgtttctgc gctccaacct gcaacaggcg tcctttaact
    36841 ttgaacgtat tcacgggctg ggtttttgct acgacatgat ccccgccatc aaacgacttt
    36901 acccattaaa agaggatcag gttgcggcgc tcaggcgaca cctggtgttc ttcaatacca
    36961 cgccagccgt atgtggcccg gtcatcggcg tcactgccgc catggaagag gcgcgagcca
    37021 acggcgcgga aattgatgac ggtaccatta acggcatcaa agtcggcctg atgggaccgt
    37081 tggcaggggt tggcgatcca ctggtctggg gaacgctgcg cccgattacc gccgcgctcg
    37141 gcgcatctct ggcactttcg ggcaacattc tcggcccgct gctgttcttc tttattttca
    37201 acgcggtgcg tctggcgatg aagtggtatg gcctacagct cggctttcgc aaaggggtga
    37261 atatcgtcag cgatatgggc gggaatgtgc tgcaaaaact caccgaaggc gcgtcgattc
    37321 tcggactgtt tgtgatgggc gtgctggtga ccaaatggac gtcaatcaac gtaccgttgg
    37381 tggtttcaca aacgcatgcc gccgatggct ccaccgtcac catgaccgtg cagaacattc
    37441 tcgaccaact ttgccctggt ttgctggcgc tcggtctgac gctactgatg gttcgtctgc
    37501 tcaacaaaaa aattaacccg gtatggctga ttttcgccct gtttggctta gggattatcg
    37561 gcaatgcgct gggcttcctg tcctgattct tcgccccggc acgactgccg gggccatcgc
    37621 tcaacatgag gtggtttatg aaaacaacag ctctgcgtct ttatggtaaa cgtgatttac
    37681 gcctggaaac ctttgatctt cctgaaatgc aggaggatga aatcctcgcg acggtggtca
    37741 ctgacagcct gtgcctctct tcctggaaag aggccaacct gggtgaaaac cataaaaaag
    37801 tacccgacga tgtggcgacc aacccaatca tcatcggcca cgagttttgc ggcgatattc
    37861 tggccgtggg taaaaagtgg cagcacaaat tccagccggg tcagcgttat gtgattcagg
    37921 ccaacctgca actccccgac cgcccggact gccccggcta ctccttcccg tgggtgggcg
    37981 gcgaagccac gcatgtggtt attcccaacg aggtcatgga acaagattgc ctgctggcat
    38041 acgacggcga aacctatttt gaaggctcgc tggttgaacc gctttcctgc gtgattggcg
    38101 cgttcaacgc caactatcat cttcaggaag gtagttataa ccacacgatg gggattcgcc
    38161 cgcaagggcg cacgctgatc ctcggcggca ccggcccgat ggggctgctg gcgattgatt
    38221 atgcgttgca tggaccggtt aacccgtcgc tgctcgtcat taccgatacc gacaacgaca
    38281 aattgagtta tgcacgcaag cactatccat cagaaccgca aacactgatt cattatctca
    38341 atgccgccga tgcagcattt gatacgttaa tggcactgag tggcggtcac ggcttcgatg
    38401 atattttcgt ctttgtgcct aatgaaggac tggtgactct cgcctcttcc ttgctggcga
    38461 cagatggttg tctgaatttc ttcgccggac cgcaggataa gcacttcagc gcgccaatta
    38521 atttctacga tgtgcattat gcatttaccc actacgtggg cacgtcaggc ggcaataccg
    38581 acgacatgcg cgcggcggtc aaactgatcg aagagaaaaa agtgcaggcc gcaaaagtgg
    38641 taacacatat tcttgggctg aatgccgcgg gcgaaaccac gcttgaattg cctgccgtcg
    38701 gcggcggcaa aaagctggtg tataccggga aatacctgcc gcttatgtca ctcacgcaga
    38761 ttcaggatca agcactggcg gcgattctgg cgcgtcatca ggggatctgg tccggtgagg
    38821 cggagcaata tctgctcgcc catgcagaag caatttccca tgattaatcg cgctacttaa
    38881 ctctgcatgt cgctggctgg tcgccgcggc aacttcgcga ccggatgtgc ggctgattgc
    38941 tgaagcggcc caatgtgcac gagaaggata aaaaaacatc agcgccttaa ggggcgctca
    39001 aattggtggc aaagtacaca ttgtttatgc cggatgcgga atgaagcctt attcggcata
    39061 cataatctcg aaaattcaat atattgcagt tatctggtag gccaggtaag cgtagcccat
    39121 caggcaatgc tgtgtttgtc atcagtctca gcgcccggtg caatacgtga catcgtgagc
    39181 catgtcagta agtggttaaa ttcgcagtgg tagcatgcct cccgagattt cttactaacc
    39241 ggagaagtaa tggaactgct tttattgagt aactcgacgc tgccgggtaa agcctggctg
    39301 gaacatgcac tgccgctaat tgctgaacag ttgcagggtc gccgctcagc ggtgtttatc
    39361 cctttcgctg gcgtaacgca gacctgggat gattacacag cgaaaacggc tgcggttctc
    39421 gctccgctgg gtgtttctgt caccggtatt catagcgttg tcgatcccgt tgccgcgatt
    39481 gaaaatgcgg aaattgtgat tgtcggcggc ggtaatactt tccagttgct gaaacagtgc
    39541 cgcgagcgcg ggctgctggc accaattact gacgtggtta aacgtggcgc gctgtatatt
    39601 ggctggagcg caggcgctaa ccttgcttgc ccaaccattc gtaccaccaa cgatatgccg
    39661 attgtcgatc cacaaggttt cgatgcgctg aatctgttcc cgttgcaaat caacccgcac
    39721 ttcaccaacg cgctgccgga aggccataaa ggtgaaaccc gtgaacagcg tattcgcgaa
    39781 ctgctggtcg tcgcgccaga actcacaatt attggtctac cggaaggtaa ctggatcaca
    39841 gtgagtaaag gtcacgcaac gctgggtggc ccgaacacca cttatgtgtt taaggctggt
    39901 gaagaagcgg ttccgctgga agcgggtcac cgtttttaaa tgaattgccg gatgcataat
    39961 ccggcatctt gttcatcgtg cctgatgcga cgctttacgc gtcttatcag gtctacaggg
    40021 tcacggtatt aatactcgtc ccgtccttcg tcttcatccg gctgttccag cacactgtaa
    40081 gccaccgaac aaaacagcga gttcagacgc tgcatatctc ccagtaagcc taaatgtagc
    40141 gaactggttt caatgctttg cacgttttgc tgatgcaggc gatcgacgtg agcatgggaa
    40201 taacggcgat taagaatgcg aaaacgatgc ttgctgcggc gcaaacgacg ggcgctggta
    40261 acatcgccag agaagaaaac cgacattgcc agttttaaat tactgagcaa ttgctcatag
    40321 agcgcatcca gttccttcaa gccatcaagc gaaaatgctc gccttgcagc cagcgatttg
    40381 tcggcaattt cgctccccat gcgctcgacg atatcggagg cctgttcaag gttgagcgac
    40441 atttcgatga tctccgccca gcggcgcgac tcttcttctg ccagttcctc ttttggcatc
    40501 cgcgccagat acagcttaat ggcggtatag agaacgttga tatcatctgc cagcttacgc
    40561 agctctttct cctgccgtgg ctcgccgtgc atcactttat tcagcccttc catcatctgt
    40621 tccatggcgt cgccaatgcg cagggtttcg cgcgcggcgt tcgccagagc aagcgtgggc
    40681 gtatccagcg cgctgacatc cagatgtttg ggccgtagct gggtatccag ttccggttca
    40741 tcgcgaataa tcgttttgca aaaccgtgcc atcgggtcaa caaatggcag catgaccagg
    40801 caacgtacaa ggttgtagaa gacatggaaa tagatcacca gttccgcttt tggcaatgac
    40861 aacttcccca ttgtctctgc cagcaaatgg acaaacggca ggataatcag gctacccacc
    40921 agcttaaaca gcagactacc cagcgcgaca cggcgggctg cggcattggc ggcactgttg
    40981 ttgagcatcg ccagcaggcc ggaaccgagg tttgcaccaa tcaccagaca aagcgccact
    41041 gggaaagaga taatgcctgc ggcggtcaga gtcgcggtca gcagtaccgc ggcaaggctg
    41101 gagtagctga taatggcgaa catcgcacca atcagcgcat ccagcagaat atcgccggtc
    41161 agcgaggcaa agatcacctg aacgccgttt gcctgggtga tcggcgttac ggcctgcaca
    41221 atcaactcca gcgccagcaa aatcaaccca agaccaatac cgacgcggcc cagttgcccg
    41281 gcgcgtgact gtttgcgtcc gaggaaaaaa atcacgccga taaaaataag taatggtgac
    41341 agccaggata agtcgaaggt gagaatacgc gccattagcg ccgtcccgac atctgcaccc
    41401 agcacaatca ccagagccgg tgcgagggct accagatcct gagcgacgaa cgaggtcacc
    41461 agcatagtgg tggcattact gctctgcacc agtgcggtaa cgcctatccc cgcgcaaaag
    41521 gcgagcggct ttttttcgac gctccggcta aggacagtac gcaaacgcgc gccgaagacg
    41581 cgcattacgc cggttcgaac aatatgagtc ccccagacca gcagggcgac ggcagaaagc
    41641 aggtgaagca gcgttaacac gagttcaggt tctccttatc gttatatgtt cctgaagcaa
    41701 ttgaatgacc aaaggaccgg aaacacgctg actggcatct ttactgtgcc ttttgacatt
    41761 cgtttttccg gctaagatta ttccagcctg tttccagtat aagtgtttca tattaataaa
    41821 gagacagggc actcattgag cgccctgttt gttgtaagga aaagtgacag atttgtgaat
    41881 cagtccgcgt cataccccag attcggtgcc agccagcgct caacatctga gacgctcata
    41941 cctttacggc gggcatagta ttcaacctga tcgcgctgga tttgcgccac cgcgtaatac
    42001 ttgctgtccg ggtggctgaa gtaccaaccc gaaaccgatg caccaggcca catggcgaaa
    42061 gattctgtga gtttcatgcc agtgtgtttt tccacttcca gcagctccca gatggtggct
    42121 ttttccgtat gttccgggca ggccggataa cccggtgccg gacggatgcc ctggtagttt
    42181 tcgcggatca gctcttcgtt gctgaggttc tcgttcggcg catagcccca gtagacttta
    42241 cgcacacgct catggagata ctccgcaaag gcttcggcta aacggtcggc aagcgctttc
    42301 accatgattt tgttgtaatc atcgtgctgc gcttcaaagg catcagccag tgcgtcctct
    42361 tccagcccgc cagtcacggc aaatgcgccg atgtaatctg ctttaccaga aagcttcggc
    42421 gcaacgaagt cagcgagaca gtagttagcg aagccggttt tttcggtctg ttgacgcaga
    42481 tggtggctga cgttgatcac atgggtacgc gtttcgtcac ggtagatttc aatgtcatcg
    42541 cccacacggt ttgccgggaa caggcccacc acgccacgcg gattcagcgt tttttcggcg
    42601 cttaatttat ccagcatgtc gttggcgtct ttaaacagcc gctgcgcctc aacacctacc
    42661 acttcatctt ccagaatgcg cggatacttc ccggccagcg accaggtcat aaagaacggc
    42721 gtccagtcga tgtaattacg cagcgtttcg atgctggctt cgacttcctg cacaccgaga
    42781 cggtgcgcca ccggcggcgt gtaagcctgc cagtcaaaag cgaagtcgtt atcacgcgcc
    42841 gcctccagcg tgaccggtgg tgtgcgtggt ttctttcgtc cgtgctgaat acgcacggtt
    42901 tcgtactcct tgcgggtacg agcaacgaaa tcatcgcgct gagtgtcgga aagcagcgcc
    42961 gccaccacac caacggtgcg tgaagcgttc tgcacataca ccgtcgggcc gctgtagttc
    43021 tgctcgattt tcaccgccgt gtgcgctttt gaggtggtcg cgccgccaat caatagcgga
    43081 atggtgaagc cctgacgctc catctctttc gccacgttaa ccatctcgtc cagcgacggc
    43141 gtgataagcc ccgaaagtcc aatcagatca gcattcactt ctttagcggt acggagaatt
    43201 ttttccgcag gcaccataac gccgagatcg acaatttcgt agttgttaca ttgcagcacc
    43261 acaccaacga tatttttacc gatgtcgtgg acgtcgccct tcacggtggc gatcaccatc
    43321 ttgccgttgg ttttgccctg ctctttgctg gcttcaataa acggttcgag gtaggccacc
    43381 gcctgcttca tcacacgcgc cgatttgacc acctgtggca ggaacatttt cccttcgcca
    43441 aacagatcgc cgaccacgtt catgccgtcc atcaacgggc cttcaatcac ttcaatcggg
    43501 cgcgtagcct gctggcgggc ttcttcggta tcctgctcga taaactcggt aatgcctttg
    43561 accagcgagt attccagacg tttattcact tcccacgagc gccactccgc ctgctgggcg
    43621 ttagcggtgt cgtcggtttt gctgccgcga tatttctcgg caagctccag taaacgctca
    43681 gtgccatcgt cgcgacggtt aagaatcaca tcttccaccg cgtcgcgcag ttcagcgggt
    43741 aggtcgtcgt aaattgccag ctgcccggcg ttgacgatcc ccatatccat gccattgcga
    43801 atagcgtagt agaggaacac cgcgtgaatg gcttcgcgca ccggatcgtt gccacggaat
    43861 gagaaagaaa cgttagatac gccgccggaa atcagtgcgt gcggcagttc acgtttgatg
    43921 tcttcacacg cgccgataaa gtcctgcgcg taattgttgt gctcttcaat gccagttgcg
    43981 accgcgaaga tgttcgggtc gaagatgata tcttcagccg ggaagccaac ctcttcggtg
    44041 aggattttgt acgcccgacg gcaaatctcg attttccgtg cgcgggtatc ggcctggccc
    44101 tgttcgtcaa aagccattac cacgactgcc gcaccgtaac gacgcaacaa tttcgcgtga
    44161 tggataaaag cgtcgacgcc ttctttcatc gaaatagagt taacaatgcc tttgccctgg
    44221 atacacttca gacctttttc aatgacatcc cattttgagg agtcgatcat aatcggcacg
    44281 cgagcgatat ccggttcacc ggcaatcaga ttgagaaaac gcaccatcgc cgcttcggcg
    44341 tcgagcatcc cttcatccat gttgatatcg ataatctgcg caccgttttc cacctgctga
    44401 cgcgccacat ccagcgcctc gctgtatttc tcttctttga tcaggcgctt gaacttagcg
    44461 gaaccggtga cgttggtgcg ttcacccacg ttcacaaaca ggctatcttc gccaatgttc
    44521 agcggctcca ggccggacaa acggcaggct acggggattt ccggcagttt gcgcggcgct
    44581 aatccttcta ctgcacgact catcgctgca atatgttgtg gcgtggtgcc acagcagccg
    44641 ccgacgatat tgagaaagcc cgcttgcgcc cattcacgta tctgttttgc catcgtgtcg
    44701 gcgtcgagat cgtactcacc aaaggcgttg ggtagcccgg cgtttgggtg cgcggtgacg
    44761 tagcattccg caatccgtga cagctcctgc acgtactggc gcagttcgtc tggccccagc
    44821 gcacagttaa ggccgaaagt cagcgcttcg gcgtggcgca gagagttata aaacgcttcg
    44881 gtggtctgcc cggagagcgt gcgcccggag gcgtcggtga tggtgccgga gatcataatc
    44941 ggcagctcaa cgcccagcgc ttcaaactcc gttttcaccg caaagaccgc cgctttggcg
    45001 ttaagggtgt cgaaaacggt ttcaatcagg atcagatccg caccaccttc caccagcgct
    45061 ttggtggatt cgcgataggc cgccaccagc tggtcaaaag tgatgttgcg gaatgccgga
    45121 tcgttgacgt ccggagaaat agacgccgtg cggttggtcg ggccgagaac accggcaacg
    45181 tagcgcggtt tctccggcgt gcgcgcggtc cactcatcag cacaggcgcg cgccagtttc
    45241 gccgccgcaa agttgatttc cgccgacagg gattccatct ggtaatccgc catcgcaatg
    45301 gtcgtggagt tgaaggtgtt ggtttcgatg atatccgcgc ccgcttcaaa gtaggcgttg
    45361 tggatagcgg cgatcacttc cggtttactg agcaccagca ggtcgttgtt gcctttgagg
    45421 tcgcatggcc agtcggcaaa gcgttcacca cgaaaatcgg cttcgttcag tcgatagctc
    45481 tggatcatgg tgcccatacc gccgtccagc accagaatac gttcatttaa ctgcgcacgc
    45541 agttgttcca ctttgctgct cacacttgct cccgacacgc tcaacccaga tttaataaaa
    45601 attcaacaaa ccatactggc ataaacgcat ctgtggtaaa agcgaccacc acgcaacatg
    45661 agatttgttc aacattaact catcggatca gttcagcaac tattgcattc gctgataata
    45721 aaaatgaaaa tgatttccac gatacagaaa aaggagactg tcatggtcgc acccattccc
    45781 gcaaaacgcg gcagaaaacc cgccgttgcc accgcaccag cgactggaca ggttcagtct
    45841 ttaacgcgtg gcctgaaatt actggagtgg attgccgaat ccaatggcag tgtggcactc
    45901 acggaactgg cacagcaagc cgggttaccc aattccacga cccaccgcct gctaaccacg
    45961 atgcagcaac agggctttgt ccgtcaggtc ggcgaactgg gtcactgggc aatcggcgca
    46021 cacgccttta tggtcggtag cagctttctc cagagccgta atttgttagc gattgttcac
    46081 cctatcctgc gcaatctaat ggaagagtct ggcgaaacgg tcaatatggc ggtgcttgat
    46141 caaagcaatc acgaagcgat tattatcgac caggtacagt gtactcacct gatgcgaatg
    46201 tccgcgccta tcggcggtaa attgccgatg cacgcttccg gtgcgggtaa agcctttctg
    46261 gcccaactga gcgaagaaca ggtgacgaag ctgctgcacc gcaaagggtt acatgcctat
    46321 acccacgcaa cgctggtgtc tcctgtgcat ttaaaagaag atctcgccca aacgcgcaaa
    46381 cggggttatt catttgacga tgaggaacat gcgctggggc tgcgttgcct tgcagcgtgt
    46441 attttcgatg agcaccgcga accgtttgcc gcaatttcaa tttccggacc gatttcacgt
    46501 attaccgatg accgcgtgac cgagtttggc gcgatggtga ttaaagcggc gaaggaagtg
    46561 acgctggcgt acggtggaat acgctgactt tttcgcctct gatttatacg aatcgaacgc
    46621 taaaccgctg cttgcgccga tacgcataaa catcttccac atgcccttca cgtatgcggt
    46681 tttgtagtgc gcgccagtaa tcagcgcgga acaagtcggc gtgcatctct tcaaatagcg
    46741 gaccaatacg cgggtcggca cataaccagt gacggaactc ttccgggaaa acatcgcccg
    46801 gcgagacgct gtaccacggt tcgctggcaa gttcgtcttc cgggtagcgc ggcagcggga
    46861 tgtcgcggaa attcacttcc gtcatgtagc aaatttcgtc gtaatcataa aacaccacgc
    46921 gcccgtgacg ggtgacgcca aagtttttaa ataacatatc cccagggaag atgttggcag
    46981 cggcaagctg gcgaatggca ttgccgtact cttcaatagc atcacgcaat tgctgcccct
    47041 caacttgctc aagccagata ttaagcggca ccatccgccg ttcgatgtag agatggcgaa
    47101 tcacaatctg ttcgccgaga tcggtgattt tttccgctgc ttcctggagc agtaattcca
    47161 ttaatgccgg ggaaatatgc cgcttctcca gcacaaagtt ttcaaactcc tgggtgtccg
    47221 ccattcggcc cacgcgatca tgctctttca ccagttggta gcaggcacga acgtgagcgg
    47281 cagacatctc tttctgcggc gcgaacctgt ctttgatgac tttgaatact cgatcaaaac
    47341 ccggcaacgt aaacaccaac atcaccattc cacgaatacc cggcgcttca atgaactgct
    47401 cattacagcc ctgtagataa acgagatatt cgcggtagct ttcggttttg gcgtgcttct
    47461 ggcagccgat agccatatac aattcagcgg tggttttacc tggcagaatt tcccgtagcc
    47521 actcgaccag cgctgcgggc agcggtgcat aaaccataaa ataagaacgc gcaaagccaa
    47581 aaacaatact cgcttcggcg gtggtcgtca ggcaggtatc aataaataac tcgccgtcgt
    47641 ccgtctggtg gatcggcagc aaaaatggca atgtgccgga aggcgtgatc agtttgccta
    47701 ccagccaggc ggctttattg cggtaaaaca gttcgttcgc cacctgtaaa tgactttccg
    47761 cgaggttgtc tgtccccagc gtttccgtca gatggcgaac gatgtaatga atgtcacggc
    47821 ttttattctg ccagcgcaga cgcaacggca ggtcgctgat aacacgcatc agcagagatt
    47881 cccagccgtg atcggggtga aagtctttcg ccagcgggcg cggaatggta cgaaaacggc
    47941 gctctggctg ggagctaaag ataaaaagcc gctcgggagt aagcgagcgg tggtcaaata
    48001 accgacagta cacggagtta aaaaagctct ccgcaatctc gaagcgcggg taatctggca
    48061 acagccgggt gtaatgctct ttaacgcgca gtaaaaatgc cgcgtccgtg ctttggccgt
    48121 tagtaatgca gcgcagttgc tccacgacca gacccacgtg atgatcgtaa agatgaatac
    48181 ggtttttcat cgcctgctgg acagcatgcc agtcggcttg ctcgaaacgc tgctgcgccc
    48241 cggaagtcac ttcgaggaat cgaccatact gagcgtcgaa accttgcaaa atggtttgtg
    48301 caatcagtaa ttccaggcca cgcggcattt gttgccctcg tcgggagcag agaattgccg
    48361 gataagacgc gtaagcgtca catcaggcgc tgattgccgg atgcggcgta gacgccttat
    48421 ccggcctacg ttcggcaacg gctgtaggca tgataagacg cgcaagcgtc gcatcaggcg
    48481 ctgattgccg gatgcggcgt gaacgcctta tccggcctac agtcagcaac gtttatcggt
    48541 tgcttagaac tgcgattctt cggtggagcc ggtcagcgcg gtaactgaag acgtgccacc
    48601 ttgaataatg gtcgtcactt tatcgaagta acccgtaccc acttcctgct ggtgagaaac
    48661 aaaggtatag ccatcttttg cagcggcgaa ttccggctgt tgcactttct caacatagtg
    48721 cttcatgccc tcgccctggg cataggcgtt tgccaggtcg aacatgttga accacatgct
    48781 gtggattccc gccagagtga taaactggaa cttgtagccc atatccgaca gctgctgctg
    48841 gaagctggca atagttttgt cgtcgaggtt tttctgccag ttgaacgacg gcgagcagtt
    48901 ataagccagc agtttgcccg gatatttcgc gtggatggct tgtgcaaagc gacgcgccag
    48961 ttccagatcc ggcgtggagg tttcacacca taccaggtca gcatatggcg catacgccag
    49021 gccacggctg atcgcttgct caatacccgc atgagtacgg aagaagcctt cactggtacg
    49081 ctcaccagta ataaattcgc tgtcatatgg gtcgcaatcg gaggtaatca gatccgccgc
    49141 atcggcatcg gtacgagcca ccagcagggt tggaacgccc gtcacatcag ctgccagacg
    49201 cgccgcgacc agtttctgaa tagcttcctg agttggcacc aaaactttgc cgcccatgtg
    49261 accacatttc ttcactgatg ccagctgatc ttcgaagtga actgccgctg caccggcttc
    49321 aatcatcgct ttcatcagtt caaaggcatt cagtacaccg ccaaaaccgg cctccgcatc
    49381 ggcgacgatc ggcaggaagt aatcgacata gcgcggatcg cccggctcaa tgcccgcgga
    49441 ccattggatc tgatccgcac ggcggaaagt attgttgatc cgctccacca ccgctggcac
    49501 cgagtttgcc ggatagagcg actgatccgg atacatgctg gccgccaggt tagcgtccgc
    49561 cgctacctgc catcccgaca gatagactgc ttcaataccc gctttcgcct gttgcagcgc
    49621 ctgaccgcca gtcagtgcac cgaggctgtt gatgtagcct tttttcgact cgccgtgcag
    49681 cagacgccac attttcgccg cgcccagttg cgccagggtg cattccggat tgactgaacc
    49741 gcgtaatttc accacatctt ccgcgctgta cgggcgagta atgccttccc aacgcggttg
    49801 agtccactct ttctgtaatt cttcaatttg ttgtgtacgg gttttcatgt gcagatgctc
    49861 catagttatg tggtggttta cgctaacagg cggtagcctg gcagggtcag gaaatcaatt
    49921 aactcatcgg aagtggtgat ctgttccatc aagcgtgcgg catcgtcaaa acgcccctgg
    49981 gagaaacgtt cttcgcccag ttcgctggca atgactttca tctcttcgcc cagcatctgg
    50041 cggaacaagg ctttagtcac cggtttgcca ttgctcaacg ttttttgatg atggatccac
    50101 tgccagattg aggtacggga aatttcagcc gtcgcagcat cttccatcag gccataaatc
    50161 ggcacacagc cgttgccaga gatccacgct tcgatgtact gcacagccac gcgaatgttg
    50221 gcgcgcatac cttcttcggt gcgttcacca tcacaaggtg ccagcagctg atcggcagta
    50281 atcggcgcgt cttgttcgcg catcacttca agctgatttt tacgggagcc gagaatgtcg
    50341 ttgaataccg ccattgccgt atccgcaagg cccgggtgag cgatccatgt gccatcgtga
    50401 ccgttattgg cttccagcga tttatccgct tttactttgt tgagcacctg gttattgcgc
    50461 tcttcatctt tgctcggaat aaatgccgcc ataccgccca tcgcaaaagc accgcgttta
    50521 tggcaggttt taatcaacag gcgtgagtaa gcattcagga agggtttatc catcgtcact
    50581 gcctgtctat ctggcaggac gcgatcggga tagtttttca acgttttgat ataactgaag
    50641 atgtaatccc aacgaccgca gttcagacca acaatatggt cacgcagcgc gtgaaggatt
    50701 tcgtccatct ggaacacggc gggcagcgtt tcaatcagca acgtcgcctt gatggtgccg
    50761 cgcggcagat taaagcgatc ttctgcatag ctgaagactt cgctccacca ggccgcttcc
    50821 tgccaggact gggttttcgg cagatagaaa tagggaccac tgccctttgc caacagtgcc
    50881 tgatagttgt ggaagaaata gagcgcaaaa tcaaacaggc taccggggat tgcctcacca
    50941 cgccaggtga catgtttttc cggcaagtgc agaccgcgta cccgacaaat caacaccgct
    51001 ggattgggct tgagctgata aattttgcct gcttcattgg tatagctgat ggtgccgtta
    51061 accgcatcgc gcaggttaat ttgcccgtcg atcactttgt tccagtctgg tgccagtgaa
    51121 tcttcgaaat cggccataaa gactttcaca ttggcgttga gcgcgttgat caccatcttg
    51181 cgctcaaccg ggccggtaat ctctacccga cgatcttcta agtccgcagg aatcccgcga
    51241 attttccagt cagcatcacg aatggaagct gtttccgaaa taaaatcagg caacgttccg
    51301 ttatcgatat cctgctgctg ctgaatgcgc gctgccagaa gtttattgcg ttgtggcgta
    51361 aaatgcgtca ccagctcagt cagaaattct accgcttcgg cagtaagaat atgcttctcc
    51421 tgctcgccat acggccttgt gaaagccaat tcatcggttg ttgttgcctg ttcagtcatc
    51481 gtgcagctcc tcgtcatgga tccgaaaact tccccactgt gaacgaagga tcgttgtgcg
    51541 cttttcgttc agcacaacta agactactca tttaaaatgc aaaatcaaaa acaatttcca
    51601 ttttaattaa aaatacttga taacttattg ataacaatat gaataaaatt tattcaccta
    51661 ctgaggtgtg tttcggaaat aaaaaaggca cccgaaggtg cctgaggtaa ggtgctgaat
    51721 cgcttaacga tcgactatca cagaagatta atccagcgtt gggttcatgt gccgtagatc
    51781 gtatggcgtg atctggtaga cgtaatagtt gagccagttg gtaaacagca aattaccgtg
    51841 actacgccag ctcgctcgcg gtgtattttg cggatcattg tgcgggaaat agttatacgg
    51901 tacatccggg tctagcccgg cttccacatc gcggaaaaat tcctgcgcca gcgtttgcgc
    51961 atcatattcg ggatggcccg tcacaaaggc aatgcgctta tctttgctgg caaacagata
    52021 tgcatcccct tcttccgtct ctgccagaat ttccagatcg gtataatcac gaatcaacgc
    52081 tgccggaaag tcagcatagc gcgaatgcgg tgccaggaat gaatcatcaa agccacgcgt
    52141 cagaagcgca tgaggatgga gaatatgatg ctcgtaaacg ccagagagtt tgtcggtgcg
    52201 ggtttgctta ggaatgccgt agaggatatt gagcgcggcc tgtacagccc agcagacaaa
    52261 cagcgtcgag gtgacgtgat ctttcgacca ctccagcacc tgtttgatct gcggccagta
    52321 agcgacatca ttaaactcca ccaggcccag cggcgcgccg gtaactatca gaccgtcaaa
    52381 gttttgttcc tgaatatctt caaagttaca gtagaagtta ttcagatgct ctgcgggcgt
    52441 gttgcgcgat tcacgggaat cgatgcgcaa cagctgaata tcgacctgca aaggtgagtt
    52501 tgaaagcagg cgcagaaact gattttcagt ttcgatcttc ttcggcatca ggttaaggat
    52561 aagcaccttt agcggacgaa tttcctgacc agacgcgcga gaagttgtca tcacaaagac
    52621 gttttcttca cgcaagaaat tgacggcggg tagctcgtcc ggcacacgaa tcggcataac
    52681 ctgattacct cactacatac gcttatacgt ttagacatcc agatagctga agataaccag
    52741 aaaatttacc aatgtcgagc ctgcatgttg aaggtgagaa agtttcaagg aaaaggctac
    52801 gttagaatat aagaatgacg aaaaggagag aaaatggtta ttagtattcg ccgctcacgg
    52861 catgaggagg gggaggaact cgttgcgatt tggtgtcgtt ctgtcgatgc cacccacgat
    52921 tttttatcag cagagtatcg ggccgagctg gaggagctag tacgttcttt cctgccggaa
    52981 gcaccgttgt gggtcgcggt taatgagcgg gatcggccgg ttgggtttat gttgttaagt
    53041 gggcagcata tggatgcgct gtttatcgat cctgatgtgc gcggctgcgg cgtgggtcgg
    53101 atgctggtga agcatgcgct ctcaatggcc ccggaactga caaccaatgt taatgagcaa
    53161 aatgagcagg cggttgggtt ctataagaag gtgggcttta aggttacgga acgctctgag
    53221 gtggacgatt tggggaaacc gtatccgttg ctgaatctgg catatgtggg ggaataagct
    53281 gccccctatc atgctatcat gcttgcgtaa gctggagcag acgcgccagc gtcgcatccg
    53341 g
//
LOCUS       DAPHCV010000014        52219 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.014, whole genome shotgun sequence.
ACCESSION   DAPHCV010000014 DAPHCV010000000
VERSION     DAPHCV010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.014"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            76..807
                     /gene="artJ"
                     /locus_tag="RH033_000851"
     CDS             76..807
                     /gene="artJ"
                     /locus_tag="RH033_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HDV2990082.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(836..1963)
                     /gene="rlmC"
                     /locus_tag="RH033_000852"
     CDS             complement(836..1963)
                     /gene="rlmC"
                     /locus_tag="RH033_000852"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HDV2990083.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPLLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMVKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(2004..2492)
                     /locus_tag="RH033_000853"
     CDS             complement(2004..2492)
                     /locus_tag="RH033_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HDV2990084.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(2552..3397)
                     /gene="potI"
                     /locus_tag="RH033_000854"
     CDS             complement(2552..3397)
                     /gene="potI"
                     /locus_tag="RH033_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HDV2990085.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            complement(3394..4347)
                     /gene="potH"
                     /locus_tag="RH033_000855"
     CDS             complement(3394..4347)
                     /gene="potH"
                     /locus_tag="RH033_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HDV2990086.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTICCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNFAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIMPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(4357..5490)
                     /gene="potG"
                     /locus_tag="RH033_000856"
     CDS             complement(4357..5490)
                     /gene="potG"
                     /locus_tag="RH033_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HDV2990087.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(5585..6697)
                     /gene="potF"
                     /locus_tag="RH033_000857"
     CDS             complement(5585..6697)
                     /gene="potF"
                     /locus_tag="RH033_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HDV2990088.1"
                     /translation="MTALNKKWLSGLVTGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(7048..7524)
                     /locus_tag="RH033_000858"
     CDS             complement(7048..7524)
                     /locus_tag="RH033_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HDV2990089.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(7612..8514)
                     /gene="rimK"
                     /locus_tag="RH033_000859"
     CDS             complement(7612..8514)
                     /gene="rimK"
                     /locus_tag="RH033_000859"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HDV2990090.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(8575..9297)
                     /gene="nfsA"
                     /locus_tag="RH033_000860"
     CDS             complement(8575..9297)
                     /gene="nfsA"
                     /locus_tag="RH033_000860"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HDV2990091.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTELLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAQYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(9281..9568)
                     /locus_tag="RH033_000861"
     CDS             complement(9281..9568)
                     /locus_tag="RH033_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HDV2990092.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMVFLGVLLMLPAAVVVILQVAKRLAPQLMNHPPQYSRSEREKDNDANH"
     gene            9728..9985
                     /gene="grxA"
                     /locus_tag="RH033_000862"
     CDS             9728..9985
                     /gene="grxA"
                     /locus_tag="RH033_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HDV2990093.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(10015..10392)
                     /locus_tag="RH033_000863"
     CDS             complement(10015..10392)
                     /locus_tag="RH033_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HDV2990094.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSHRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            10662..12347
                     /locus_tag="RH033_000864"
     CDS             10662..12347
                     /locus_tag="RH033_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HDV2990095.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESKQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(12522..13058)
                     /gene="rcdA"
                     /locus_tag="RH033_000865"
     CDS             complement(12522..13058)
                     /gene="rcdA"
                     /locus_tag="RH033_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HDV2990096.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAPGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            13142..14350
                     /locus_tag="RH033_000866"
     CDS             13142..14350
                     /locus_tag="RH033_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2990097.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            14350..15165
                     /gene="ybjI"
                     /locus_tag="RH033_000867"
     CDS             14350..15165
                     /gene="ybjI"
                     /locus_tag="RH033_000867"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HDV2990098.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDATKTVAEMYYHRLEYVDNFDNLDDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFNQ"
     gene            15251..15529
                     /locus_tag="RH033_000868"
     CDS             15251..15529
                     /locus_tag="RH033_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2990099.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQNVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYTAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(15570..16802)
                     /gene="mdfA"
                     /locus_tag="RH033_000869"
     CDS             complement(15570..16802)
                     /gene="mdfA"
                     /locus_tag="RH033_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HDV2990100.1"
                     /translation="MQNKLASGVRLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSLYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            17087..17683
                     /gene="ybjG"
                     /locus_tag="RH033_000870"
     CDS             17087..17683
                     /gene="ybjG"
                     /locus_tag="RH033_000870"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDV2990101.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            17741..18499
                     /gene="deoR"
                     /locus_tag="RH033_000871"
     CDS             17741..18499
                     /gene="deoR"
                     /locus_tag="RH033_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HDV2990102.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLIEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(18546..19748)
                     /gene="dacC"
                     /locus_tag="RH033_000872"
     CDS             complement(18546..19748)
                     /gene="dacC"
                     /locus_tag="RH033_000872"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDV2990103.1"
                     /translation="MTQYSSLLRGLAACSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGQSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            19994..20620
                     /gene="gstB"
                     /locus_tag="RH033_000873"
     CDS             19994..20620
                     /gene="gstB"
                     /locus_tag="RH033_000873"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HDV2990104.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(20617..21732)
                     /gene="yliI"
                     /locus_tag="RH033_000874"
     CDS             complement(20617..21732)
                     /gene="yliI"
                     /locus_tag="RH033_000874"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HDV2990105.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRMVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDLGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(21843..22226)
                     /gene="bssR"
                     /locus_tag="RH033_000875"
     CDS             complement(21843..22226)
                     /gene="bssR"
                     /locus_tag="RH033_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HDV2990106.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            22439..23764
                     /gene="rimO"
                     /locus_tag="RH033_000876"
     CDS             22439..23764
                     /gene="rimO"
                     /locus_tag="RH033_000876"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDV2990107.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWRKICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            23990..24376
                     /locus_tag="RH033_000877"
     CDS             23990..24376
                     /locus_tag="RH033_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDV2990108.1"
                     /translation="MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVI
                     KKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSGLSSKGTKEIEDDELAAYKKMAN
                     AFLAFSNKQIEDLIETGFLIEVKNER"
     gene            24366..24695
                     /locus_tag="RH033_000878"
     CDS             24366..24695
                     /locus_tag="RH033_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HDV2990109.1"
                     /translation="MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAK
                     VKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSKWERNEIKPSGPALRILNTLAIK
                     GPEVFAK"
     gene            complement(24765..26093)
                     /locus_tag="RH033_000879"
     CDS             complement(24765..26093)
                     /locus_tag="RH033_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDV2990110.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKSVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYFQVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(26101..28449)
                     /locus_tag="RH033_000880"
                     /pseudo
     CDS             complement(26101..28449)
                     /locus_tag="RH033_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(28627..29538)
                     /gene="gsiD"
                     /locus_tag="RH033_000881"
     CDS             complement(28627..29538)
                     /gene="gsiD"
                     /locus_tag="RH033_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HDV2990111.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(29541..30461)
                     /gene="gsiC"
                     /locus_tag="RH033_000882"
     CDS             complement(29541..30461)
                     /gene="gsiC"
                     /locus_tag="RH033_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HDV2990112.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(30479..32017)
                     /gene="gsiB"
                     /locus_tag="RH033_000883"
     CDS             complement(30479..32017)
                     /gene="gsiB"
                     /locus_tag="RH033_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HDV2990113.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(32037..33908)
                     /gene="gsiA"
                     /locus_tag="RH033_000884"
     CDS             complement(32037..33908)
                     /gene="gsiA"
                     /locus_tag="RH033_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HDV2990114.1"
                     /translation="MPHSDELDAGDVLAVENLNIAFMQEQHKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDQMLLRRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMIEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGSVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     SGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPKHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(33895..34860)
                     /gene="iaaA"
                     /locus_tag="RH033_000885"
     CDS             complement(33895..34860)
                     /gene="iaaA"
                     /locus_tag="RH033_000885"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDV2990115.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     AMVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            35064..36299
                     /gene="moeA"
                     /locus_tag="RH033_000886"
     CDS             35064..36299
                     /gene="moeA"
                     /locus_tag="RH033_000886"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDV2990116.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASPLKKSPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEAGEWVEVEPFNALFGGL"
     gene            36299..37048
                     /gene="moeB"
                     /locus_tag="RH033_000887"
     CDS             36299..37048
                     /gene="moeB"
                     /locus_tag="RH033_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HDV2990117.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(37136..37798)
                     /gene="fsa"
                     /locus_tag="RH033_000888"
     CDS             complement(37136..37798)
                     /gene="fsa"
                     /locus_tag="RH033_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDV2990118.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWQGAFGRT
                     SI"
     gene            37929..38828
                     /locus_tag="RH033_000889"
     CDS             37929..38828
                     /locus_tag="RH033_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDV2990119.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDTRLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHFTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            38834..41266
                     /locus_tag="RH033_000890"
     CDS             38834..41266
                     /locus_tag="RH033_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HDV2990120.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            41412..42227
                     /gene="ybiV"
                     /locus_tag="RH033_000891"
     CDS             41412..42227
                     /gene="ybiV"
                     /locus_tag="RH033_000891"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HDV2990121.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGQQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNIAPFNC"
     gene            42379..43638
                     /locus_tag="RH033_000892"
     CDS             42379..43638
                     /locus_tag="RH033_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HDV2990122.1"
                     /translation="MTFSCEMLPTDAKAAIREMKAALREQLSDVQQVFDTLSAKIETR
                     VAEIDALKAQGLPVWPEVSYSDIAAGTVSDATRNEIKRRGCAVIKGHFPREQAMAWDR
                     AMLDYLDINHFDDVYKGPGDSFFGSLEASRPEIYPIYWSQAQMQARQSENMAAVQSFL
                     NRLWKSESNGKQWFNPDISVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQRV
                     FANVFNGNFDDYDPWDAAHRTDVEEYTVDNTTKCSVFRTFQGWTALSDMLSGQGLLHV
                     VPIPEAMAYVLLRPLLDDVPDDELCGVAPGKVLPISEKWHPLLLKALTSIPAITAGDS
                     VWWHCDVIHSVAPVDNQQGWGNVMYIPAAPMCEKNRAYAHKVKMALEKGASPGDFPRE
                     DYEANWEGRFTLEDLNIHGKRALGMDV"
     gene            complement(43879..45471)
                     /locus_tag="RH033_000893"
     CDS             complement(43879..45471)
                     /locus_tag="RH033_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HDV2990123.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGELDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            45690..46610
                     /gene="ldtB"
                     /locus_tag="RH033_000894"
     CDS             45690..46610
                     /gene="ldtB"
                     /locus_tag="RH033_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDV2990124.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(46669..47787)
                     /locus_tag="RH033_000895"
     CDS             complement(46669..47787)
                     /locus_tag="RH033_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HDV2990125.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGAQTPEWKPRLVWSCLGLYIVFLMALE
                     LKQELWGLVIVAVGFTLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLSNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(47784..48251)
                     /gene="mntR"
                     /locus_tag="RH033_000896"
     CDS             complement(47784..48251)
                     /gene="mntR"
                     /locus_tag="RH033_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HDV2990126.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            48437..48565
                     /gene="mntS"
                     /locus_tag="RH033_000897"
     CDS             48437..48565
                     /gene="mntS"
                     /locus_tag="RH033_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HDV2990127.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            48837..50420
                     /gene="opgE"
                     /locus_tag="RH033_000898"
     CDS             48837..50420
                     /gene="opgE"
                     /locus_tag="RH033_000898"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDV2990128.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPHVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKAHWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDNLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(50469..50984)
                     /gene="ompX"
                     /locus_tag="RH033_000899"
     CDS             complement(50469..50984)
                     /gene="ompX"
                     /locus_tag="RH033_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HDV2990129.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(51037..51102)
                     /locus_tag="RH033_000900"
     CDS             complement(51037..51102)
                     /locus_tag="RH033_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2990130.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            51337..>52219
                     /gene="rhtA"
                     /locus_tag="RH033_000901"
     CDS             51337..>52219
                     /gene="rhtA"
                     /locus_tag="RH033_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HDV2990131.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDI"
BASE COUNT        13145 a        13727 c        12677 g        12670 t
ORIGIN      
        1 ttcacttgat ggcattgtta tcccatgccg cagacacggc caaaaatcat aagattgaca
       61 gacgggagtt ccatcatgaa aaagttagtt cttgccgctt tacttgcttc ttttactttc
      121 ggtgcttctg ccgcagagaa aatcaatttt ggcgtttcag ccacctatcc accctttgaa
      181 tctataggtg ctaataatga gattgtcggc tttgatatcg acctggcaaa agccttgtgc
      241 aaacaaatgc aggcagaatg tacttttact aatcacgcgt tcgacagcct gatcccgtca
      301 ctgaaattca gaaaatatga cgccgtaatc tccggaatgg atatcacgcc ggagcgtagc
      361 aaacaggtat cgtttaccac gccctactac gaaaactcag ccgtcgtgat tgccaaaaaa
      421 gatacctaca aaacgtttgc cgatctgaaa ggcaaacgta ttgggatgga aaacggcacc
      481 acgcaccaga aatatattca ggatcagcac ccggaagtga aaaccgtctc ctatgacagt
      541 tatcagaatg cctttatcga tctgaaaaat ggccgtattg atggggtatt tggtgacaca
      601 gcggtggtta atgaatggct gaaaaccaac ccgcaactgg gcgttgcgac tgaaaaagtg
      661 accgatccgc aatacttcgg caccggcctg ggtatcgctg tacgtccgga taacaaagct
      721 ctgctggaaa aactgaataa cgcgctggca gcaattaaag ctgacggtac atatcagaaa
      781 atcaatgacc agtggttccc gcagtaagat tcagaaagcc gcacctgcgg cttttttatt
      841 gcttaaccag cagtgtcagc acttcgtaat gcgcagtgtg cgggaacata tcaaaaagct
      901 gtacccgttc gatgcggtaa cctggcagtt cgcggatatc tttcaccatg gtttgggcgt
      961 tacagctgga gtagataata aaacgcggtg ccatcgttga gagataatca cacagcggtt
     1021 taccgatgcc gcggcgcggc gggttaacca gcaccagctc cggcacctcc ccctgagcag
     1081 tggcaaactg agtggagtcc agcgcctgaa attgcaaacg cgttaagccc agttcagcgg
     1141 ctgactgctt tgcacaggca atggcctctg gcgcaatttc gatcccggtt aactgcatgt
     1201 caggcgtcgc gcagtgtaaa ccaaagcccc ccacaccgca gaacagatcc cacatatgtt
     1261 taaccggcag ctgtcgtacc cagtcgcgcg cggtggcgta caactggctg gcgaccgccg
     1321 gattagtctg gaagaaactt tgcggacgga tccacagcgg tacgtcatta aaacgctctg
     1381 ccagcgcctg ttgttcagtc aggtaaatct ccgtctcccc ttccataatc gccatatgta
     1441 ccggctgaat attgacggta ataactttca gctgcggtag ttgttcctgt aacaacggca
     1501 gcgccttacg cagttgcgcc agcttggtat cagaacgcag tacaaagcgc agcatcatgc
     1561 cgccatcgct ctggctttca gtcagcagaa tgtatttcag ttcgccacgc ttacgcgcca
     1621 cgttgtaggg cgttaacccc gctcgggcga taaacggttt tagcgccgca aaaacgggcg
     1681 caaatgaggc aggataaagc ggacagtcac aaaggtcttc cggcgtgccg tcgcgatgca
     1741 gcataccgag cagtggtttt tcaacgctac cactcaccac cattttggct ttattacgaa
     1801 acccttgttc cgggcctgac accggcgcgc accattcctc aaccggaaag tcggcgagca
     1861 gatttttaag atcggcggtt ttagcggaga gttgctctgg aatcggctgc gttatccact
     1921 gacaggaacg acagcgaccc gcgtcgtaaa gtgcgcactg catacattga ccttcacatc
     1981 atcaggggcg acgattatac acattattgc aactggaaga accgccgact ggtcgaggga
     2041 acgaacagca gcatcaggat gagcatatcc ggcaacttct gcagcatcag actatggaag
     2101 atttcacgtt ttgattcacc gggaatgctg aacagctccg gataaccata ccccagcgag
     2161 gccgcccaca agtaacttgc ggctgtgatt tgtgtcaaca gatacagcca gcgcgcccag
     2221 cgacgccctt tcaccagtga aaacgcacac cagatctcaa tgaaaaccag caccagactg
     2281 ctcaaaaaga ccagcgttaa actccaggtt tgtacgctgc gatgaatgaa ctcgccaata
     2341 ccgcgcacgc ccagcgtatt gaaaatcatc agcacgtcga ggccacggat cataataatg
     2401 gcgagcgccg ccacctgcac cagcgcaggc acattcaggc gagcatgaga tgaagatgtt
     2461 ttcttaaaaa atcccaacgt ttcgtcttcc atgaaaacaa tgccgcgaca tgcgcggcat
     2521 tatgtagcca ggttggcaaa ttttagtgtc ttcagccacg tcttgcacgc tggatgtcgc
     2581 gtatccgctg tttttctgcg cgcgccatca gataccaggc gataaatccg acaattccga
     2641 ccgcgccgag gatcaatgtt gccagggcgt tgatttccgg attcaccccc atccgcacgc
     2701 tggaaaagac cagcatcggt aacgtggtgg ctcccggccc ggaaacaaag ctggcgatca
     2761 ccagatcatc aagcgacaaa gtaaaagcca gtaaccagcc agaaatgatc gcgggcatga
     2821 tcatcggtag cgtaatgaca aaaaacactt tcagcggcgt cgcaccgaga tccatcgcgg
     2881 cttcttctat cgagcgatcc agttcccgca gacgcgacga aataacgact gccacataag
     2941 ccgtacagaa agtgacatgc gccagccaga tggtgagcat accgcggtcc gcaggccagc
     3001 caatagcatg agcaagcgcg acgaataaca acaacagcga caaccccgtg atgacatctg
     3061 gcatcaccag cggcgcggtg atcataaagg caaaaccatt tgatccgcga aacctgccaa
     3121 aacgcaccaa caccaccgcc gcaatcgtcc cgaggatcgc cgctgccgtt gccgcacagg
     3181 ctgcaattgt cagacttaaa ccaaccgcac tcatcatcgc gtcatcgcgc aataattcgc
     3241 cataccagcg cgttgaccag ccggcccaca ccgtcaccag tttcgagctg ttgaatgaat
     3301 agataaccag catcagcatt ggcgcgtaga gaaaggtgaa gcccagcagc aaaatcacaa
     3361 tccgccaggg cgaacgaaca accggtaaat tattcatccg tgttctccca cgcttttttg
     3421 ctggtgtttg tgaaaccaca ttatcggcac aattagcagc agcaacatga tgatcgctac
     3481 cgccgaggct accggccagt cgcggttgtt aaagaactcc tgccacagca cgcgcccgat
     3541 catgatgctg tccgggccac cgagcagttc cgggatcaca aactcgccca cagcagggat
     3601 aaacaccagc atcgatccgg caataatccc ccctttggtg agtggcatga tcacggtaaa
     3661 gaacgttttc agcggtcgtg caccgagatc cagtgccgct tctaccagcg aataatcaat
     3721 acgaatcaac gcggtgtaaa tcggcagcac cataaacggc acgtaggcgt aaacaatgcc
     3781 aatataaacc gcaaagttgg tatgcagaat ggtcagcggt tgatcgataa cccccagcca
     3841 cagcagaaaa ttattcagca caccgttgtt ttttaatatt cccatccagg cgtagacacg
     3901 aatcagaaac gaggtccacg acggcaggat caccagtagt aataagatat tacgggtcga
     3961 aggcttactg tgcgccaccg cccacgccag cggatagccg atcagtaaac agcaaatagt
     4021 cgaaatcgcc gccacctgta acgactggag ataagcatca aaatagagcg gatcgtcggt
     4081 cagttgcaga aaattaccga gattaagagt gatagaaagt tgcccgtcag cccactccat
     4141 caattcggta taaggtggaa tagcgcgcgc catctccgcc aggcttattt taaagacgat
     4201 cagaaatggc agcagaaaca gcaagatcaa ccagatatat ggcaacgcaa tgaccagttt
     4261 gcgcccatgc ttcatttgca gctgcgacaa ccacagctta aatccgcccg gcggtttcga
     4321 ctgggcagca ggttcaagtg tactcatctt tgctccttaa accgtcagca ccacacagct
     4381 gtccacttcc cagcacaaac gtacttcgtc gccccaggtc ggtaaccctt tacgatgacg
     4441 atgggcgttt tgtagctggg cgctgatcat ctgcccactt ttcagacgaa cgtgatacac
     4501 cgaaagatcg ccgagatagg caatgtgtat cacctccccc accgcgaagt tacaaccatt
     4561 ggcgggcggc tcttcgcaaa gcatgatttt ttccggacgc agcgctacgt gtaccggcac
     4621 gttatcaacc actgaagcat ctgcatcgac tttcagtggg tgcaccagtc ccggcgaata
     4681 aagcaccaga ccatcttcct gacgctcttt gagtacacct tcaaagacat taaccgaacc
     4741 gataaattca gcgctatagc gggtggtcgg atgctcgtag atctcttccg gttcgccaat
     4801 ctggacaaat ttcccacgat tcataatggc gatacgcccc gccatggtca tcgcctcttc
     4861 ctgatcgtgg gtgaccatca cacaagtcac accgacacgc tccagaatat ccaccacttc
     4921 aagctgcatc cggtcacgca gctttttatc cagcgcgccc atcggctcat cgagcagtaa
     4981 tagtttcggg cgcttggcaa ggcttctggc cagggccaca cgctgacgct gaccaccgga
     5041 aagctgatgc ggtttgcgtt tggcgaactc ctgcatatgc accagcccaa gcatctcatt
     5101 gacccggctg gcaatttccg ctttcggtag tttgtcctgt ttcaggccaa aagcgatgtt
     5161 ctgttccacg gtcatatgcg gaaacagcgc gtaagactga aacatcatat tgatggggcg
     5221 caggtaaggc ggcacctgtg acaaatcgac gccatcgagc ataatctgcc cggcagaagg
     5281 ttgttcaaaa cctgccagca tacgcagcag cgtggacttg ccacagccgg atgcgcccag
     5341 cagcgcgaag atttcacctt tgtagatggt caggctgaca tcatccaccg catgttgacc
     5401 atcgtaggat ttggtcaggt tacggatttc taatagcggc gtcagcgcct tacgggtttt
     5461 cgcctgcggg cgagggatag cgtcattcac ggctgctctc cggcattgaa tgtgtgcaag
     5521 cgcaccatga tggtgcgaat tgccggcggg tgtggtgcgc ccgtccggca tctacggctg
     5581 cggattattt tccgctcttc actttggtcc acgcgcgggt gcgcacacgg tcgattttcg
     5641 ggtcctgcac tttcagagtg aacagcttcg cacgaacatc cgcaggcgga taaatacccg
     5701 ggttatcacg gacttccgca ctcaccagcg gcgtggctgc tttattggcg ttagcataga
     5761 acacatggtc agagatatgt gccaccacat caggacgcag caggtaattc aggaactgat
     5821 aggcttcgtc tttgtttttg gcatccgcag gcatggcgaa gacatcaaag aacgccatcg
     5881 ccccttcttt tggaatcgag aacgagacat tcacgccatt cttcgcttcc ttcgcgcggt
     5941 ttgacgcctg ccagacatcg cccgcccagc caatagcgac acagatgtca ccattcgcca
     6001 gatcattgat gtattgggat gaatggaaat aacgaatgtt cgggcgcagc tttaacagca
     6061 gatcggtagc cggtccggtg taatcatccg ctttagtgct gttaggatct ttgccgagat
     6121 aattcagcac ggtagcaaac acttcttctg gcgcatcgag gaaagagaca ccgcagcttt
     6181 tcagtttttc cagattttca ggtttgagga tcaagtccca gctatcgacg ggcgcgtttt
     6241 cgcccagcac cgctttaact ttatcgacgt tatagccaat cccggtcgtc gcccacatat
     6301 agggcatagc aaatttattg tcgggatcgt gtttggcgac caacttcagc agctccggat
     6361 cgagattctt ccactccggc aatttgcttt tatccagcgg ctggaaaact cccgcagtca
     6421 actggcgttc aagaaagctt gctgacggca ccaccaggtc aaagccggtg ctacccgcca
     6481 ttaatttgcc ttccagtact tcgttagagt cgaaaacatc gtagacgact ttaataccgg
     6541 tttctttttc aaaattggcc accgtgtccg gggcgatata atcagaccag ttataaatgt
     6601 gaagtgtttt ttgttcagcc gcgagcgtgc cgacagagac ggccatcaga gcacccgtaa
     6661 ccagacccga tagccatttt ttatttaagg cggtcatatc tctttccttc tgaaagttcg
     6721 ttaacaaacg aacaaaagtt gtcacactga aagatatgca ataaacgtgc atatttttta
     6781 tcggcgcggt gtaagaaaag agaactctct cccggccagc actgctcgct atataaaagc
     6841 atcgcaagaa taactttagc cagggtttga gactatagct cggattaaaa aacggtattt
     6901 aacaaatttt tatgcgtatt tgctctatgt gataaagcca aatcggcaca agagaagtga
     6961 attaatcttt aataaaaggt taaaaacagc ggataatttt gcggcacgca gtcactgcgg
     7021 cagcgactgc gtgctcgaaa ggagttatca gtggaggaaa tagttttcgg taacgttatt
     7081 ttgcccttca tcgtcagtcg gtaacagcag ttgatgggca ttagcttcaa gaatgaccat
     7141 cgaaatctgc tcttcgctct gacgcacgaa ccatgcaaac tgctcatagg ttacgccctg
     7201 cataacggat aaagactggc aaaccaccag ctttggcaga ttatcatcct gcatatcaag
     7261 aaatgctttc acggtcagcg aactggcatt gatggctgat aaatccgccg ccagcggcaa
     7321 tacggctgaa ggtcggactt ccgccatggc agaaaacagg atcgtgttat cgatcagatc
     7381 gattttagca tcaaagacac cgtcgaaatt ctgcatatgg ggcagatgca gagcctgaca
     7441 gttatcacat tcaaaaaaac tcatccccag gtcatcgagc cattgacgca acgtatccag
     7501 accaggaacg accagcgatg tcataatttt gtacaacctc ttccgataaa aagaccggca
     7561 cagcttacgc aaaaagcgca ggcaaaacca tgatcagtaa tgtgattgcg actaaccacc
     7621 cgttttcagg caatattctg tcgtagcgtg gcgttcgatc cagcggatca ttttacccgc
     7681 gatgtcgata ccggtggttt tttctattcc ttccagccct ggcgacgcat tcacttccat
     7741 caccaacggc ccgcgattag cacgcagaat atccacacca gcaacgtcca gcgccatcgt
     7801 tcgcgcggct tttatcgcga tttcacgctc ctgtggtgtg atactggcga cgcttgccgc
     7861 gccgccacga tgcaaattgg aacgaaaatc gccctctttc gctcgccgtt caatcgcagc
     7921 gactacttca tcgccaacaa ccagacagcg gatatcgcac ccttgcgcct ctttgatata
     7981 ttcctgcacc agaatatgcg cgttcagacc gcggaaagcg tcaatcacgc tttccgccgc
     8041 ctgacgcgtc tccgccagca cgacaccaat tccctgcgtg ccttcaacca gcttgaccac
     8101 cagcggcgca ccaccgacca tgtcgattaa atcgctggta tcatccggcg aatgcgcaat
     8161 gcccgtgaca ggcaggtcga tgccctgacg cgccagcagt tgcatggaac gcaatttgtc
     8221 acgcgcccgg gcaatggcga ccgactcatt gagcggatag ctccccagca tctcgaactg
     8281 gcgcagtgcc gccgtcccat aaaaggtaat ggcggtgcca atacgcggga tcactgcgtc
     8341 aaaatggggt aacttgcggc ctttgtagtg aatagaagac gccgcaggat ttatgttcat
     8401 gtagcaagaa agcggatcaa gaatttcaac caggtgaccg cgctgtatag cggcttcacg
     8461 cagccgctta cacgaataga gcgttccatc ccgggacaat atggcaattt tcaccctgca
     8521 cctctctgtc agacctggtc aaaagcccgc gtatcataca tcgacgtggc agttttagcg
     8581 cgtcgcccaa ccctgtttgt gcaaataatc cagaataaat gggcggcttt ctttaatgat
     8641 tgttcggcgg atatgatcgc tccaggtatc ccggcgatta ttgctgccac gagtgaggta
     8701 atattgcgcc agttgctcgt catactgcgc cagtgcgtct ttatccagcg gttgatagct
     8761 gttttcatgc accaaaatgg aggccggtaa acgcggctta agatccggat tatccgcagg
     8821 ccagccaagg cacagcccaa acagcggcag aacatgctgc ggtaatttca gcagttccgt
     8881 caccgcttca atattattgc gcaggccgcc gatatatacc ccgcccaatc ccagcgattc
     8941 cgctgcggtt aatgcattct gcgccatcat tgccgtatca acgacaccga gcaacagttg
     9001 ttccgccagg ccgagctgag catccggaca gatctgtaaa tgacggttaa agtcggcaca
     9061 gaacacccaa aactccgccg cttgcgctac gtgtttttgc ccgccggtca gcgtcaccag
     9121 ttcttcacgt aacgctttgt cggtaatgcg aataatgcta ctgcactgca aaaaactgga
     9181 actggacgtc gcacgggcgc tgttaataat cgcctcacgc tgcgcttcgg aaatgggttc
     9241 atcagtgaaa tggcgaatgg agcgatggcc acaaataagt tcaatggttg gcgtcattat
     9301 ctttttctct ttctgaacgt gaatattgcg gtggatggtt catcagctgt ggggcaagac
     9361 gttttgccac ctgaagaatc accaccaccg cagcgggaag catgagcaaa acaccgagaa
     9421 aaaccatcag aatctgcact tctggtcgag aaaatggctc aggtagcgac agggagtcgc
     9481 ttaccgacag cagcgccacc gccagcaaca tcattccgat aaattccagt atcaacacac
     9541 ttttaggcaa tttaccgatc gcgcgcatac gcttccctct gcaaagtgag ccttcagtct
     9601 aaaacttttc actgtattgt gtttaacagt tatagctttt agcaattaat gcaacaggtt
     9661 aaacctactt tcagcgaata cattttagcg tgatcattac aggtataaat ctatgaggag
     9721 agaaataatg caaaccgtta tttttggtcg tccgggttgc ccttactgtg tgcgtgcaaa
     9781 agatctggct gagaaattga gcaatgaacg cgatgatttc cagtatcagt atgtagatat
     9841 tcgtgcggaa gggatcacta aagaagatct acaacaaaag gcaggaaaac ccgtagaaac
     9901 cgtgccgcag atttttgtcg atcagcaaca tatcggcggc tataccgatt ttgctgcatg
     9961 ggtgaaagag aatctggacg cctgatcgtc tgacaagccc tcgcgttgag ggctttattg
    10021 attttttctg tgctgtggtt taaacaaact actgataaat aagaaacaca atgcccccag
    10081 cgcacaccag aacaccgcac ttaataacca tgccagctct tgccagaatg agcgcgtcgg
    10141 tgaaaaaaac agccgcataa tgagcatcga acagggtgcc gccagcattg caccaaacag
    10201 aggtttcagg acttctctac gatgtgaaaa gaagctggca actgcaccag gaagaatgaa
    10261 aaatagcaaa ccgatttcag gatgcccggc agcccgaaaa gcgcctttca tgtgcgtcgc
    10321 cagaaaaaga cacaccacaa tgaagaggac aaaacagcag attgcccccg cccaacgttg
    10381 tttatgtttc actcgttcct cctgacactg cgtctatcga acacattttt cgccagtgtg
    10441 gcgttcagta agataaagcc gcttcgcatt ccatgctaat ataggccaac gcaattcata
    10501 tagccgttga tacctaatgt gattacacta gtaaaatata ttgttacttt actatcgttt
    10561 aggtgcgctg aatgaatctg cgccctgaat tctggtaaaa aacattatcg taaattacca
    10621 tttctttcaa cagcttacta gtaaacaaga agttagcctc cgtgaatata aacgtcgccg
    10681 aattgttaaa tgggaattac attctgttat tatttgtggt cctcgcgctt gggctatgtc
    10741 tcgggaaatt acgacttggt tcgatccaac tgggtaattc cattggcgtt ttagtcgtat
    10801 cgctgttatt aggccaacaa catttcagca ttaacaccga cgcgcttaat cttggcttta
    10861 tgctgtttat tttctgcgtc ggggtcgaag ccggaccgaa ctttttttcc attttttttc
    10921 gcgatgggaa aaattaccta atgttagcac tggtgatggt tggcagtgcg ctggtcatcg
    10981 ccttagggtt aggtaagctg tttggctggg atattggcct gacggccggt atgttagcag
    11041 gctctatgac gtcaacaccg gttctggtcg gtgctggcga tacactgcgt cattccggca
    11101 tggaaagcaa gcagctctca ttggcactgg ataatctgag cctcgggtat gccttaacct
    11161 atttaatcgg cctggtgagt ttgattgttg gtgcgcgtta cttgccgaaa ttgcagcatc
    11221 aggacttaca gaccagcgcc cagcaaatcg cccgcgaacg tggcctggac acagatgcca
    11281 accgtaaggt ttatttgccg gtgatccgcg cctaccgcgt cggcccggag ctggtagcct
    11341 ggaccgacgg caaaaatctg cgtgaactgg gtatttatcg acaaactggc tgttacattg
    11401 aacgtattcg gcgtaacggg attctggcaa atccagacgg tgatgccgta ctacaaatgg
    11461 gcgatgaaat agcgttggta ggctatcctg acgcccacgc ccgactcgat cccagtttcc
    11521 gtaatggcaa agaagttttc gatcgtgacc ttctcgacat gcgtatcgtc actgaagaag
    11581 tggtcgttaa aaaccataac gctgttggca aacgcctggc acaactgaag ttgaccgatc
    11641 acggttgctt ccttaaccgc gtcatacgta gccagattga gatgccgatt gatgacaacg
    11701 tcgtgcttaa caaaggtgac gttttacaag tcagcggcga tgcccgccgg gtaaaaacca
    11761 tcgccgatcg catcggcttt atctcgattc acagccaggt cactgacttg ctggcattct
    11821 gcgccttctt tgttattggg ctgatgatcg ggatgatcac cttccagttc agcacattca
    11881 gtttcggtat ggggaacgct gccgggttgt tattcgccgg aattatgctg ggctttatgc
    11941 gtgctaacca cccgaccttc ggttacattc cgcagggtgc attaagcatg gtgaaggagt
    12001 tcggcttgat ggtgtttatg gcaggcgttg gtctgagcgc tggtagcggt attaataacg
    12061 gcctgggcgc gattggcggt cagatgttga ttgccggatt gattgtcagt ctggtgccag
    12121 tggttatctg cttcttgttc ggtgcttatg tattgcgaat gaaccgcgca ctgttgttcg
    12181 gcgcaatgat gggcgcacgc acctgcgcgc cggcaatgga gatcatcagt gatacagctc
    12241 gcagtaatat cccggcactg ggctatgcgg gcacttacgc aatcgccaac gtcctgctga
    12301 cgctggcagg gacaatcatc gtcatggtat ggccaggatt aggataaaac tgaagttgcc
    12361 ctgaaaatga aatttttttg cacaaccgca gaacttttcc gcagggcatc agtcttaatt
    12421 agtgccactg cttttctttg atgtccccat tttgtggagc ccatcaaccc cgccatttcg
    12481 gttcaaggtt ggtgggtttt ttgttgccta aaatttatct actaccctgc aaccctctca
    12541 accatcctca aaatctcctc gcgcgatagc ggcttacggt cggtgacaaa atgcagcgtc
    12601 atcccctcaa taaacgcatc aagcgcgcgg gcggttccgg gttcaaacca ttgttcgagc
    12661 gtttgctgac tgcgctgcat ccagttttgc attaccgttt ttaatagcgg ttttcggcta
    12721 gccagcgcgt agagctggta catcagctcc atgttatccg gcgttgcaac ctgtgagctg
    12781 taaatcatat cggtgatcgc ctggcatgcg cccggagcat cactaacatc gctaaaaaat
    12841 gcctgatatt gccgggacat gatctcagta aaactgctga acgcctccag caacaactca
    12901 tcaattcctg aaaaatagta ggtcatcgac cccaacggta ccccggcaag ggtagcaatt
    12961 ttgcggtgcg taacagcatg tattccataa agtttcaccg cctccagcgt ggcctggata
    13021 attttttctc gccgttgcgg atcgttagcg cgacgcatgt catttcctct ctctgcaatt
    13081 gtgtacaaat gtacacaacc ttgctaatgt tgtcttccct cttcttattt tgacgtgatc
    13141 tatgaccgta aattcttcac gtaatgcatt gaaacgccga acctgggcgc tgtttatgtt
    13201 cttctttttg ccaggcctgt taatggcgtc ctgggcaacc cgtacgcctg ctatccgcga
    13261 tattctttct gtttcgatcg ctgaaatggg cggagtcctc tttggtctgt cgatcggttc
    13321 aatgagcggt attctctgct cggcgtggtt agtgaaacgc tttgggacgc gtaatgtcat
    13381 cctggtcacg atgtcctgcg cattgatcgg gatgatgata ctaagtctgg cactctggct
    13441 gacatcgccc ctgctcttcg cggttggtct cggcgtcttt ggggcaagtt ttggttctgc
    13501 ggaagtggcg ataaacgttg aaggtgctgc cgttgagcga gaaatgaata agacggtttt
    13561 gccgatgatg cacggttttt atagcctggg cacgctggca ggcgctggtg tcgggatggc
    13621 actaacggcc tttggcgttc cggcaacggt gcacatttta ttggcggcgc tggtaggcat
    13681 cgcgcctatt tatatcgcca ttcaggcaat ccctgatggt acgggcaaaa atgctgccga
    13741 tggcacccag catggtgaaa aaggcgtacc tttttatcgc gatatccagt tgctgctgat
    13801 tggtgttgta gtgctggcga tggcctttgc cgaaggttct gccaacgact ggttaccctt
    13861 attaatggtt gacggtcacg gttttagccc tacttccggc tcgctgattt atgccggttt
    13921 taccctgggg atgaccgttg gacgctttac cggcggttgg ttcattgacc gttacagtcg
    13981 cgttgccgtg gttcgggcca gtgcactaat gggggcgttg ggtattgggc tgattatttt
    14041 tgtcgatagc gcctgggttg ctggggtgtc tgttgtactt tggggactgg gtgcctcgtt
    14101 gggcttcccg ctgaccattt ctgccgccag cgataccggc cccgatgcac caacccgcgt
    14161 cagcgtggta gctacgaccg gttatctggc tttcctcgtc gggccgccac tgctgggcta
    14221 tctcggcgaa cattatggat tacgtagtgc aatgctggtt gtactggcgc tggttattct
    14281 cgcggctatt gtcgcgaaag ccgtcgccaa acccgatacc aaaacgcaga cggcgatgga
    14341 gaatagttga tgagcattaa attaattgcg gtagacatgg atggtacttt cttaagcgat
    14401 caaaaaacct ataaccgtga gcggtttatg gctcagtatc agcaaatgaa agcacaaggc
    14461 attcgctttg tggtcgccag cgggaatcaa tattatcagt tgatctcttt ctttccggaa
    14521 attgctaatg aaatagcctt tgtggctgaa aacggcggct gggtagtgag cgaaggcaaa
    14581 gatgttttta atggcgagct gtcgaaggat gcgtttgcta ctgtcgtgga acatttgttg
    14641 acgcgcccgg aagtggaaat tattgcctgc ggaaaaaata gcgcctatac cctcaaaaaa
    14701 tatgacgatg ccacgaaaac ggtggcggaa atgtattatc accgtctgga atacgtcgat
    14761 aactttgaca acttagatga tatcttcttt aagtttggtc tgaatctttc cgatgaactg
    14821 attccacaag tacaaaaagc attacatgag gccatcggcg atattatggt gccggtccac
    14881 accggcaacg gcagcatcga tctgattatc cccggcgtac ataaagccaa tggccttcgc
    14941 caactgcaga aattatgggg aatagacgac agcgaagtgg tggtctttgg cgatggcggt
    15001 aacgatattg agatgctgcg tcaggcaggc tttagttttg caatggaaaa tgccggcagc
    15061 gcggtcgtcg cagcggcaaa ataccgggca ggctccaata accgtgaagg cgtactggat
    15121 gtgatcgata aagtgcttaa acacgaagcg ccatttaacc aataaaaacg gcagcacgcc
    15181 cggcaacaca atgcctgaat gaaggctgca taattcgcgg aaattaaccc gctgcattat
    15241 ttcaggcgaa atgattatga aaaattgtct actgttgggc gcgcttttaa tgggctttac
    15301 tggtgtggcg atggcgcaaa atgtcaccgt cgatgtgccg agtggataca aagtggttgt
    15361 ggttcctgat tcagtcagcg ttccacaagc ggtcagcgtc gcgactgtgc cgcaaaccgt
    15421 ctatacggct cctgctccgg cttaccgccc gcatccttat gttcgccatc ttgccagcgt
    15481 aggtgaaggg atggttatcg aacaccagat tgacgaccat caccattaat tcgagaatgc
    15541 ctgatcgcac aaagcaatca ggcttttttt tacccttcgt gagaatttcc catctgttta
    15601 tcttttaaaa agataaccat cagcgacagc cacaaaattc cgttgacaag gttgaagaga
    15661 ttaaacagtc cgttgccccc gttcagccag gcatgtttgc tgatttcaat accgacggta
    15721 aagatcagca tttgcagcat tcccatcgcc gcagaaaccg tacctttact catatcgctg
    15781 gcaaacaggg ttaatcgcac cagtcccgca ttcgccagac caatgccgaa agcataaaga
    15841 cttaacccgg cggtcatcca taaatacgcg tgcgatgaga taaccgttgc cgcagcagcg
    15901 accaacagac caatcataat cggccatccg cccataataa tcagcgaacg tacggtgcgg
    15961 cgcgaggtca gacgcgctag cagcaagtta cccgcaatta acgccccgaa aataggcact
    16021 tgcagcaagc catattcata gctgctcaac tgctcaccgg taataatgat aatcggcgac
    16081 tgggcgatcc acgccaacaa cggcaggcta acgaatccca gcgccagcgc ccccgccaca
    16141 aagcggccgt tcttcagcac cagcttatag tcacgaccga gttctttcag cgacagtttc
    16201 tcgcctatac gcgtggcggt ttcaggcata gctcgttgca gaccgaaaaa ggagatcgct
    16261 gccaatgcgg caaacaagac aaacatccct tcccagggca gcacatggat ccacgccgcg
    16321 cccaccagcg gaccaagtag tggagcaatc agcgccacgt tcgccattag cgcggtgatc
    16381 ttgatacaaa ccgcctcttc gaaggattcc tgaattgcgg cgtatcccac agcgccaatg
    16441 aaacagaggc ttatgccctg caagaagcgc aacagggtga attgttcaat gttttgtgcc
    16501 agtaatattg ccagacaggt gacgataaac cacaccactc ccgccagcat caccggacgg
    16561 cggccaatac gatccgacaa cggccccagg agccattgta aaaacatccc gcccgccaga
    16621 tacgcagtca tcgaagtagg aacccaatca atgcccgcct gatattgttc caccacggcc
    16681 aacataccgg gttgaatcat atcgttgccg atataggttg aaaattcgta aagcaccaga
    16741 cagagaggga aaagtaacgc ctgacgtcca agcctgacac cggaagctaa tttattttgc
    16801 atgcaatttc ttcgccaata ataatcgcgc aaagtttaat aaaagcgcag ctaacgagaa
    16861 agcgaatttt gtagctgaaa ccacggttaa gcacattctt acattattgc gagtatagct
    16921 acgctttctt taagttttat ttaacctttg cccgttacaa tcacctaccg taaacaggcc
    16981 gcttgaggga aataagacga tgccgcttta cccagtttaa cctgcacttt attctcaacg
    17041 acttgcctgt attggctccc ttttaatcac tttgcgtcgg gaagttatgc tggaaaattt
    17101 gaatctctct ctattctctc ttattaacgc aacgccagac tctgcgccgt ggatgatctc
    17161 gttggcgatt tttattgcta aagatttgat taccgtggtg ccgttgctgg ccgcagtact
    17221 ttggttgtgg gggcttacag cacaacggca actggtgata aaaatcgcta tcgcgctggc
    17281 ggtcagcctg tttgtgtcct ggacgatggg acatcttttt ccgcacgacc gaccctttgt
    17341 cgaaaatatc ggctataact tcctgcatca tgcggcggat gactcattcc cgagcgatca
    17401 cggtacggtg attttcacct ttgcactggc atttttatgc tggcatcgcc tgtggtccgg
    17461 ctcactttta atggtgctgg ccgtcgtcat tgcctggtcg cgcgtttatc ttggcgtcca
    17521 ctggccgctg gatatgctcg gtggattgct ggcaggtatg attggctgcc ttagcgccca
    17581 gattatctgg caagcgatgg ggcataaact ctatcaacgt ctgcaatcgt ggtatcgctt
    17641 ctgttttgca ttaccgatcc gcaaaggctg ggtgcgtgac tgatctctgc taaaaagtgt
    17701 agtattgagc ggctcgcttc aataactatt cagagggatt atggaaacac gtcgcgaaga
    17761 gcgtatcggg cagctgctgc aagaattaaa acgcagtgat aagttacatc ttaaagacgc
    17821 cgccgccctg cttggggttt cggagatgac gattcgtcgc gatctgaaca accacagtgc
    17881 gcccgtcgtt ttgctcggcg gctatattgt tctggaacca cgcagcgcca gccattacct
    17941 gttaagcgat caaaaatccc gcctggtgga agaaaaacgc cgggcggcaa aactggctgc
    18001 gacgctgata gaacccgatc agaccctctt ttttgactgt ggcaccacca cgccgtggat
    18061 tattgaagca attgataatg aaatcccttt taccgccgtt tgttattcgc taaatacttt
    18121 tctggcgctg aaagagaaac cccattgccg cgcgtttctt tgcggtggtg aatttcacgc
    18181 cagcaacgcc attttcaaac ccatcgattt tcagcaaacg ctgaataatt tttgcccgga
    18241 tatcgctttt tattccgctg cgggcgtgca tgtcagtaaa ggcgctacct gctttaatct
    18301 tgaagagttg ccggtaaaac actgggccat gtcgatggcg caaaagcatg tgctggttgt
    18361 cgaccacagt aaatttggta aggtgcgtcc ggcgcgcatg ggtgacctga aacgctttga
    18421 tattgtggtg agcgattgtt gcccggaaga tgagtatgtg aagtacgctc agacgcagcg
    18481 tattaagttg atgtattaat gacgtataac cggatgacgt ttcgcgccat ccggttatca
    18541 gaagattaag agaaccagct gccgaaccac tgatggaatt tcatcataac gaaatcccac
    18601 atccgaccaa agaatccgcc ctcttccaca ttttccatca cgatcagcgg acgctgctca
    18661 atggattgac cgttaagctg gaaatcaatg gtcccgacaa cctgaccttt tttcagcggt
    18721 gcggtaagct gcggttccgt taacgtgtag ctcgctttca ggtttttcag ctgcccacgc
    18781 ggtatggtca ctgagcccgc ttcgcctgcc ccgagattca cttcgctctt atcaccaaac
    18841 cagacgcgct gagtcacaaa ggtggcatca ggtttaattg gcgtcacggt ttcaaagaag
    18901 cggaaacccc aggtcaataa tttctcagac tcattaaaac ggatacggtc agttttcgca
    18961 cccagtacta cggagatcaa acgcatatcg ccctgggtag ccgaagcaac cagattatat
    19021 cccgcgcctg ccgtggttcc tgtcttcatg ccatcaacat tcagattgct gctccacagc
    19081 agacggttac ggttaggctg tcgaattttg ttgaaggtga actctttctc tttatgaatg
    19141 gcatactctt ccggcacatc gtggatcaat gctttaccca gcaatgccat atcgcgcgcg
    19201 gtactgaact gccccggcgc atccagaccg tgcaccgtct ggaaggtagt gttggtcaga
    19261 cccagttttt tggcataacc attcatcaga ccaataaatg actcctggct cccggcaacg
    19321 taatcagcca gcgcaataca ggcgtcatta ccggactgga taatcacacc tttgttcaag
    19381 tctgccaccg aaacctgatc gcccggtttg aggaacatta ccgatgaacc acgcagtgcc
    19441 ggatttcccg tcgcccaggc atctttaccg accgtcacca tatcggtgag tttaatctta
    19501 tcggccttaa gcgcctgccc aaccacatag ctggtcatga ttttagtcag gctcgcgggg
    19561 tccagttttt catccgcatt gccttctgcc agcactttac cgctggcgta atccattaaa
    19621 atccatgcac gcgcatccac gctcggcgct tcaacggttt gttccgccgc gaatgccgtt
    19681 ggggcaaaaa ggaataaaaa tgcagaacac gctgcaagac cacgaaggag agaggagtat
    19741 tgcgtcataa atgccacccg agtatccatt caaaaaaaat acgttatatc gccgtatgat
    19801 aagttcggtg attaataacg cacacacaaa cttaaagaaa ccgtaagttg gtaaagtttt
    19861 taaagtttat gcaatatcct gccgcttcgc tgcttctggt gtcatttttt tgccagctgt
    19921 tgcttaaaag ttaactttac ttcaccatcg gagtcctgtt cagccgtggc tgcctgtcgg
    19981 aaaaggggtg agtatgatta cgctgtgggg tcggaataat tcaaccaacg taaaaaaagt
    20041 attgctgacg ctcgaagaac tggaactacc ttatgagcaa attctcgcgg gccgtgagtt
    20101 tgggataaat cacgatgctg attttctggc gatgaaccct aacgggctgg tgccgttgtt
    20161 gcgtgacgac gaaagtgatc tcattctttg ggaatcaaac gccattgtcc gctatctggc
    20221 ggcacagtac gggcaaaaac gcctgtggat cgactcaccg gcacgtcgtg cggaagccga
    20281 aaaatggatg gactgggcaa accagacgct cagtaatgct catcgcggga tcctgatggg
    20341 attagtcaga acaccaccgg aagagcgcga tcaggccgcc attgatgcca gttgcaaaga
    20401 gtgcgacgcc ctgtttgccc tgctcgatgc ggaactggca aaagtaaaat ggttctcagg
    20461 cgacgagttt ggtgtgggcg atatcgctat cgcaccgttc atctacaatt tgtttaacgt
    20521 tggcctgacc tggacaccgc gtccaaatct gcaacgctgg tatcagcaac tcactgaacg
    20581 ccccgcagtg cgcaaagtgg tgatgattcc cgttagctaa ttgcgtgggc taactttaag
    20641 taattcccca ctggactcgt cggtgagaac gtataaataa ccgtcgggtc cagtgcgaac
    20701 atcacgaatt cgctgccctc tgtccgttaa aatacggcca tcttctgtca ctttgtcgcc
    20761 gttgacgctc atcacaatga catctttatc tttcagcgcg ccaataaata atttttgctg
    20821 ccactggggg aatttgtcgc tgttatagaa ggccatgccg ctcacggcgg gcgaatcttt
    20881 ccagtaaaaa acaggttgct cggtcccggc gacgatctcc cctttcgcct ccggtatctt
    20941 aaagcctgaa tagttgattc cccaggttgc cagcggccag ccgtagtttt tgcctttttg
    21001 cgggatatta atttcatcgc caccgcgcgg gccatgttca ttcagccata gtgcattact
    21061 ccacggattc atcgccattc cttgcggatt acgaatgcca taagaccaga tctcggcgcg
    21121 cacaccagat tcctttataa aaggattatc atccgggatt tctcccaggt cggtcagtcg
    21181 caccagtttg ccctgtaatt tatccagatc ctgcgccgtc gggcgctgat tgttttcgcc
    21241 cagagcaata aaaagataac ctttaccgtc aaataccatc cgcccgccaa aatggttgcc
    21301 ggtagacagt tttggcatct gacgaaagac ggtgcggaag tcggtcactt ttgagagatc
    21361 atcacttaag cggccataac ccacagcagt tccggctttg ccatcatcgc caacttcgga
    21421 ataacttaac cagatgcggc gcgactgagc aaaatcgggc gctaaaacca cgtccagcag
    21481 gccgccctgc ccgtgcgccc aaacgtccgg cactccggaa agcggcgcag ataatccttt
    21541 tcctgcttgc cagtgacgca actcgccgcc gcgcagagtg attaacatac cgtgattgtc
    21601 gggtaaaaag gccagcgccc agggatggtc gagtttgtcc tgcagtactt cgacatttac
    21661 cgttgcagga gccgcccaga gagcggaaga aagacaaata aggggcacaa ggaaaaagga
    21721 ttgtcgatgc ataacgcctc cggattgact ctggcttaaa gcgtagtcag tggaggagat
    21781 aacaaattta tttttacaaa aacttaaaca tgaaggggga gacgctttct cccccttagt
    21841 tttcaggcct tctcaagcat ggcgtgcttc tgcaggctct ggatactcag cgttaagctc
    21901 atcagacaat tttcaagctt atcggcgttg acggtaataa cagtcgggca atcatggtgc
    21961 ccactcatca aacatactgc ggctgtcgct aatgcttctt cagcatgatg aagagcactc
    22021 cactcttcct gatccagatg aagattcaac cgcagcgatt tatcgtgcag ttcgcgattc
    22081 agtttaaaaa agttatctcg tagatgattg ctttcgctga cggacatgta tccttttgcc
    22141 ttcctcagtt gcacgtatgc ggcgaggtca acgcgtgcgt cgatcaaccg gttcagcaga
    22201 tcgattcgct gtctgtcaac gaacatactt cgatcctcct ctttccagcc ttattgcaat
    22261 taaatagtat ggctattttt tgagcaaaat gctgtccagg atcaattctt cacgccgaat
    22321 taacaaaatt tacatttcct tcgcctccgc cagcgaatga gattgcgata caaattttat
    22381 ccagaactct gtagaatccc tgccctgacc tttcctgtaa ctgaatattt tttaagctat
    22441 gagcaaagta actccccagc cgaaaatcgg ctttgtttcc cttggctgtc cgaaaaacct
    22501 tgtcgattca gagcgtattc tcaccgaact acgcactgaa ggttatgacg tggtaccgag
    22561 ctatgacgat gcggacatgg tgatcgtcaa cacctgcggc tttattgaca gcgcggtaca
    22621 agaatcactg gaagccattg gtgaagcgtt gaatgaaaac ggcaaggtaa ttgtgaccgg
    22681 ttgtctgggg gcaaaagaag atcagatccg cgaagtccac ccgaaagtgc tggaaatcac
    22741 cgggcctcat agctatgagc aggttctgga gcacgttcat cactacgtgc caaaaccgaa
    22801 acacaaccca ttcctgagcc tggtgccaga acaaggcgtg aaactgacgc cgcgtcatta
    22861 tgcctatctg aaaatttctg aaggctgtaa tcaccgctgc accttctgca ttattccgtc
    22921 tatgcgcggc gacctggtga gccgtccgat tggcgaagtg ttaagtgaag cgaaacgtct
    22981 ggttgatgcg ggcgttaaag agattctggt gatctcgcag gatacttccg cctatggcgt
    23041 cgatgttaaa catcgtactg gcttccacaa cggcgagccg gtaaaaacca gcatggtcag
    23101 cctgtgcgaa cagttatcga aactggggat ctggacacgt ctgcactacg tttaccctta
    23161 tccgcatgtg gacgacgtca tcccactgat ggcagagggc aaaatcctgc cgtatctgga
    23221 cattccgttg cagcacgcca gcccgcgcat tctcaaactg atgaaacgtc cgggttctgt
    23281 agatcgccaa ctggcgcgca tcaaacagtg gcgcaaaatc tgcccggaac tgaccctacg
    23341 ctcaaccttt attgttggct tccctggcga gacggaagaa gatttccaga tgctactcga
    23401 cttcctgaaa gaagcacgtc tggatcgcgt tggctgcttt aaatacagcc cggttgaagg
    23461 tgcagacgcc aatgccctgc ctgaccaggt tccggaagaa gtgaaagaag aacgctggaa
    23521 ccgtttcatg cagttgcagc agcaaatttc cgccgagcgc ctgcaagaga aagtgggccg
    23581 tgaaattctg gtgattatcg acgaagtgga cgaagaaggc gcgattggtc gcagcatggc
    23641 agatgcaccg gaaatcgacg gcgcggttta tctcaatggt gaaaccaacg ttaagccggg
    23701 tgatatcctg cgtgtgaaag tcgagcacgc cgatgagtac gatttgtggg gtagccgggt
    23761 ttaattaatc ttaaggcaga tctaacaagg atattgttct tcgatctgcc tctgctaccc
    23821 ttcccgatgt cctaccctta cgcacaaaat cccctgacgc ataaatcgag aaaaatgaat
    23881 cgtactcgag gcaactctca ctttttcaca ccaaattaaa gttttgatcg caattacttg
    23941 aaataagcgt aatggttact atagtaaaca ttaccacaag gatggatgta tgggtgtgta
    24001 taaggcaaga cgattcagcc aaagcacgaa aaaacttggt attcacgata aagttctcat
    24061 ggctgccgct gaagaagtga tgcagggcat atgggaggct gatttaggta gcggtgtcat
    24121 caaaaaacgt ttacccctcc aacaaggaaa gagtggtgga gccagaacaa ttatcttttt
    24181 taaatcagca aatcatgtgt ttttttatga tggttggtcc aagagtggtc taagcagtaa
    24241 aggaaccaaa gaaatcgaag atgatgaact ggcggcctac aaaaaaatgg caaatgcatt
    24301 tctggccttt agcaataagc aaatagagga tttgatcgaa actggctttt tgattgaggt
    24361 gaaaaatgag cgataaatta caaatgatgc tggaagatgc aaaggaactg aatgcactgg
    24421 gcctgatgtc tgatagtgaa gttgatgcca ttgccgtcat ggtcaaagct cgtgaagtca
    24481 gcgcccgtat agccaaagtc aaatcaatga cgggtgaaga gatcaaagcc atccgcaccc
    24541 gatacggcat gtcacaatcc gtgctggcac atacaatggg aatgtcaaaa gaaagtgtct
    24601 cgaaatggga acgtaatgaa ataaaaccga gcggcccggc attacgtatt ttgaatacat
    24661 tggcaataaa agggccggaa gtttttgcaa agtaaaatgt ttgcaaatga tttaccacat
    24721 tcattagtac cccttgatta aggggtacta acactattct aattctactg ccagctaatc
    24781 tgtgccctgc cgtttttctt actcttatac agccgctcat ccgccagcat tttcaagcgt
    24841 tcgacggtag tacattcatt tgaatgcgct acaccgatac tggtgcttaa attaatgctt
    24901 cccacctgga aatgaacaat ttcttttaga atcgatagca actcttccag cgttcccccc
    24961 gtataaatca ccgcaaattc gtcaccgccg aagcggtaga ctttaccttt atctaccggg
    25021 atctgtgatt taaggatttt caccactttc atcaacactt catcgcccag caaatgaccg
    25081 aaggtgtcgt taatgttttt gaatttatcg acgtcaaaca ggcacaaata accgcctttt
    25141 tccggcagcg cttttaaatc ttcgtcaaag gattttctgc ccatcgcttt ggtcagatcg
    25201 tccaggaatg ccgagttata catactgtag agttttttga catcggcagc cgaaaagaaa
    25261 agcccgccat ccagtacgcg ttcaaaatgg ttattaataa tggcttcttt ctccacaata
    25321 ccacgattaa tcactctgcg taccagatag aggcaactgg cacccgatat cactgccacc
    25381 aatgcggcaa agaaaataat caatgtacat acctgaaaat aaacgtcttt tttataagca
    25441 aatacgtaca acccggtcgg caaataggcg aagtgctcca tcgcttccga aacagtgaag
    25501 atggcgtttt tagcgttata ttcatcaccg gtcaccgatt taccgttctt gaacagggag
    25561 aacatcaggt tctcgctatc catcgccagc gattttgaca gtaaagaaaa acgttccaga
    25621 ccaagcaaaa atatcagact acgattgtga gttaccggat agaggtaata gacgaaggtt
    25681 ttaccatcaa aagagataaa ccctgtattt ttgacatgtc gatacttcac aacctgcata
    25741 atatctgcac aataattttt ctcgaactcc tttacctgaa tacattcgtc actggaaatc
    25801 agataaggac cgacatcgta caaataagag ggtgaaaccg agtcggcgta agctgtgcgt
    25861 aagccaacca acagctgttc ctccgtggga tttagcgtgt tgataagatc aattgccacg
    25921 ctacgccgat attcagcaat atttgccacc gcaaaagcag tatcaataac ggactgtttc
    25981 aggctataca cccgttcctt taccatttga gtgtagatcc cgcaggcaac tgctgaaatc
    26041 atgataaaaa tcagcagact gactgtaagt acgaacttat tgattctgga catacgtaaa
    26101 ctactctttt actaattttc cacttttatc ccaggcggag aatggcatgg gtttctggta
    26161 cagatacccc tgagcctgaa cacatcctaa tttgattaat tcgcgcgcaa cgtcttcgct
    26221 ctctacgcct tccgcaatca ccgggatccg gttatagcgt gccagatcga aaataatgcg
    26281 cacaattttt cccgcagtac cgaaaatatc gccggtaaat tgtgagtcca cttttatgct
    26341 atctggatta atttcataaa aacgtttcag gtttgacaaa ccggtgccaa agtcatcaat
    26401 cgatatccgt accccttgtt tacgcagttg ttcaatattg agataaaaat gttcattaat
    26461 tcgctcgatc acggtttcag tgatctcaac acagaggctg ttcgccttca ggccattttc
    26521 ttccatgatg gtggtaaaac gctcatgaaa gtgtggttcg tttaactgcc agggcgaaac
    26581 attaatatgc agtaagaaat catccttcag cgatgcacgc tgactaaaat gtctgaattc
    26641 acccattgcc gttttaataa tctgataacc cagctcattg ataaacccga tatcttcagc
    26701 aatggtaatg aacttcagcg gtgaaatgat cccccgctcc gcagacaccc aacggcacaa
    26761 cgcctccgcg cccagaatgg cgcgggtgtt aatatcaacg atcggttgat agacgaggaa
    26821 tagttcgcca atttccattg catggcgaat attacgcgca atccagatat tctcttctat
    26881 ttcgttgagt ttcgcatcat cacagaaaat aaactctgag aacatatggt tgcggatact
    26941 ggaaatcgcc agtttcagct tttctgcgta actccaggcg ttttccccct gcaaaggttc
    27001 acaaatagca gacttatgga tgcgcagatt gcggtagcca aaactgctgt caaaatcgtt
    27061 taccgtcgct atcttacggg taaacgtctg tacattttcc cggcacagga gtaaatagag
    27121 atcggcatta aatgtcacga tatcgcagca ttgcgcatat ttctcggcaa ttcgctgaca
    27181 caatttcctg acgattttat tgcgttcatc aacaccaaac accgcttcca cagcttccag
    27241 accacacagt ttgatgatcc ccacagccag atactgagta ttgttggact caataatcgc
    27301 catattttta ataaaaccgc tttcctgatc gtaagagatc agtgagagca atttatttga
    27361 ccgttcaatt tcggcatctt tcatttccgc gatcgaattt ttgatgctga taatctcttt
    27421 cgcatcgcct tcgcgaattt tcagagcctc gatctgatcc gttgagtcgg gcaaaaacgc
    27481 cagcgtatta accagctcgg tcatttcaga aaattgccga cggaacacac gagtggtcat
    27541 taaataaacc accaacaata cgataataat gaacgcaaac ggcagataaa aaagtttatc
    27601 tgatgcgttc aataaattac cgccaggata ataaaactat atttcgaata atgcgttgtt
    27661 tggcactact gatgtttttt cgatatattc agtatcgaca gaatcaataa tattataatt
    27721 accttcgcta ttataaataa tcagtttttc acgcgcgagt tgattcttgg caatgatctc
    27781 atggccttgt ttcaaaaata gaacttcatc attagcgctg atttgatcaa gatactcgtt
    27841 cagatatcgc cagtttaaat cgacatgaat tttatctact gctaacaaat catgatcttt
    27901 gccgttgatg cgtagcgtaa aagagataca ttttttaccc ggacgatcaa agcaattgta
    27961 tgccggattc cagctgttgt tagagtcatc tgaagccacc gaccatggtc gtttcgatgg
    28021 attaaaatcg gcaaaaataa cccgccctga ctcgtcgaca agcggtccat ttacttcttc
    28081 ctgatgataa acaacgattt tatcattcgc cctccgcgaa aagagataat atttattatc
    28141 atcaagtacc agtcccaccg agtctaaaaa tggcatcatt tccattttca ggatcacagc
    28201 gcgttgaatc tcctctgtat tttttaataa atacagtgag tcctttaatg tcagggccga
    28261 gcgttcaata tctttaaaga aatagccaac gtttttctga ataagttcaa agcggctatc
    28321 ggcacgtttt accgcgcgtt ccgataccag ttgatagtta atgatgaaaa aaaggccaat
    28381 aaatgacagt gctgccaata aaaataaaat tatcagcctt atctttatct tttcgtataa
    28441 actcagcatg ttaatacatt taacaaaata actgtctgaa cgctgtgccg ctgcaaaaac
    28501 agtcccttgc tgcggctttt taaccaaatt acccgattct acaaaatcag cagaaagtta
    28561 caacacttgc ttagggcaat aatttcctgt gaggtgatta ccctttcaag caatattcaa
    28621 acgtaactat cctttaattt tcggatccag cgcatcgcgt aaaccatcgc ccaacaaatt
    28681 gaacgccagt acggtcagaa aaatagccag ggccggaaaa acagcgacat gcggcgcgat
    28741 aaccatatcc gctcgtgcct cattgagcat tgctccccac tctggtgtcg gcggctgcgc
    28801 accgaggccg agaaatgaca ggctggcggc agagataatc gaggtgccaa tgcgcatggt
    28861 gaaaaacacc acgatagacg agacggtccc aggcaagata tgacgcaata aaatggtcat
    28921 atcgctggca ccaatactgc gtgctgactc aataaaggtt tgctgtttca acaccagcgt
    28981 gttgccgcgc accaggcggg caaaggcggg gatggaaaaa atggcgactg cgataatcac
    29041 gttagcaatg ccgcttccca acaccgcaac aacagcgatc gccagtaaaa tacccggaaa
    29101 ggcaaacagc acatcgcaaa tgcgcatgat cagccgatcc caccagcctt cataatatcc
    29161 agccaacaag cccagcaacg tcccgatcgc cgcaccgata aacacggcaa acacgcccgc
    29221 cgccagcgag atttgcgcac caaccaggac acggctgaaa atgtcacgcc ccagtgaatc
    29281 gacgccaaac cagtgctgca aagaaggtcc gttattcaga ttgtcataat caaaataatt
    29341 ttcggcgtca tagggagcga tccagcgtgc aaaaatagcc accacaatca ataaaataac
    29401 gaacaatgcg gcggtcatcg ccatatgctg acggcgaaat cgtcgccaga attcatgcca
    29461 cggtgtacgt acctggtcag gtttgaccag tggcatggcg tttaacaccg cctgacgtcg
    29521 ccagttaaat agtcgcatcc ttacttgtac ctgatagccg ggttaatggc ggcgtaaagc
    29581 acatccacca ctaagttgat aagaataaat tccagcgaga aaagcagaat ttccgcctga
    29641 atcaccgggt aatcgcgcat ttctacggag tcaacgagta agcgtccaag tccgggccag
    29701 ttgaaaactt tctccaccac gatggaacca ccgagcaaga agccaaactg taagcccatc
    29761 atggtcacta ccgggatcat cgcgttgcgt agcccgtgtt tgaggacaac ccaggtttcg
    29821 ctcacccctt tcgccctcgc ggtacgcata taatcttcgc ttaaaacatc gacaaacgac
    29881 gcgcgggtaa agcgcgccat cacggctgcc actgccgcgc cgagcgtcag ggagggtaaa
    29941 atgtagtgct gccaactgtc tgctcccacg gtaggcagcc agcccagttc aacggagaat
    30001 acctgaatta aaagcatccc cagagcaaat gccggaaacg agatccccga caccgcaatg
    30061 gtcatactca atcgatccgg ccaacgatta cgccagacgg cggcgataat tcccgccgcc
    30121 ataccaaata tcaccgccca gaccatactg gttatggtca gccacagcgt tggcataaag
    30181 cggctggcaa tctcatcggc aaccggacga cgcgacacca tcgacaggcc aaaatccccc
    30241 tgcacagcat tgctgatata gtgccagaac tggtgataca ggggctgatc caaccccagc
    30301 tgctgacgca ccagttctat aacctgcgca tcagcttcag gcccggcaat caatcgcgcc
    30361 ggatcgccgg gcagcatatg gacaaataaa aacaccagca ccgagacgat aaacagcgtc
    30421 ggaatcaacc ccagtaagcg tttgataacg taattaagca ttccactccc tgcgttgctt
    30481 attgcaaatc cgcgtcttca aagctgaagc cggtgtctgg catgatccaa aaaccggtca
    30541 ggtttttact gtgtgccgac accagttttt ctaccaccag cgggatccac ggcgactctt
    30601 gccagatgat atcctgcgcc gccttataca agcgggtctt ttccgccgga tcattagttt
    30661 tcagtgcctg agccaggaag tcatccacct gtttattgct gtaaaacgcg gtattaaaca
    30721 gcgtcggtgg ccagttctgc gaggcaaaca gcggcgatag agcccagtca gcttcgccgg
    30781 ttgaagccga ccagccagtg tagaacatcc gcacgccgct ctctttttgc cctttacctt
    30841 caacttctgc cgcccgctgt ccggcatcca tcgcagtcac ctgggcttta atcccgacct
    30901 gcgctaactg ctgctgggta aattgcagca ctttctgcgc ggtgctgtgg ttatgtgacg
    30961 accacagcgt ggtactgaaa ccgttgggat atcccgcctc tttcagtaat tcgcgcgctt
    31021 tcactggatc gtaaggccac ggtttataac tttgcgcgta ggcgatactc ggcggtacca
    31081 caccagtagc tggcgttgca tagcccgcga aggccacttt caccagcgcc gggcggttaa
    31141 tggcgtaatt cagcgcctca cggaccttcg ggttatcgaa tggcttttgc gtcacgttca
    31201 tactgatata acgctgcata attgacggac tggccatcaa ctcgatattt ttgtttttct
    31261 ccagcagtgt ggcctgctcg tagggaatgg ggaaagcaaa ctgcgcttca ccggtttgca
    31321 acattgccgc gcgggtgttg ttatccgcca ccggacgcca ggttatgctg tccagtttgg
    31381 gcaatcctgg ctgccagtaa cccgcgaatt ttttcacctt cacaaaatcg gtctgattcc
    31441 aggtatccag ttcatacggc ccggttccca ccggatgaaa accaatctcc ttgccatatt
    31501 tttccagcgc tgccggtgaa atcatcgcgg tcgccggatg ggcaagaata ttaataaacg
    31561 ctgagaacgg ctgtttgaga gtaatcttta ccgttgtcgg atcgatcgcc tccgttttgg
    31621 caatattctt atacaggttg tagcgtttaa gatgattcgc cggatcgctg gcccggtcca
    31681 gattcgcttt taccgccgct gcgttgaaat cggtgccatc ctggaattta attccttccc
    31741 gcagtttcac ggtgtaagta aggccgtcat cggaaacggt ataactctcc gccagcacgt
    31801 ttttcagttt catctcttta tcgagaccga acagcccctg gtaaaacgat ttcgctacgg
    31861 cctgagataa cgtgtcattc gcgtcatatg gatcgagcgt ggtgaagttc gatcctaccg
    31921 ccaccaccac atctttggca gcgaatgcac aagatgccat caacgctgtc gcaatgccca
    31981 gcgccactaa cccactacgg tgtacagctc ttgccatttt attctccgcc tgaatgttat
    32041 ctacgcatga atgcgtattc tgattgtggt tgtgcgacgt aatgccccgg cccgacgcat
    32101 tgcaacgaga cggctgcaac ctcttcgcca cgcagatgaa tattgctggg aagatcgtcc
    32161 gacagcagta cacgctgcgg tcgttgtcgg gacggttcag cgaccggaac tgccgccagt
    32221 aatttacgcg tataaggatg cttcgggttt tcgaagaccg cgcgccgtgg accaatttca
    32281 acaatttgtc cgagatacat caccgccaca cgatgactaa tccgctctac cacggccata
    32341 tcgtgggaga taaacagata cgcaatgccg aaatcacgct ggagatcgag caacaagttg
    32401 ataatctgcc cgcgaataga aacatccagc gccgagacgg cttcgtcggc aatgatcact
    32461 tttggattca atgccaacgc gcgagcaatg cagatgcgct ggcgctgacc gccggaaaac
    32521 tcatgcgggt aacgccaggc atgttccggt aacaggccca cgcgctccag caaccacgca
    32581 acgcgtgcag ccgcttcttt acctggcaat aaaccgtgta cgcgcagcgg ttcgataatc
    32641 gaatcaccga tggtctgacg tgggtccagc gaagcgtaag ggtcctgaaa aataaactga
    32701 atatcccggc gcaaagcctg aagtttaccg gatgacaagg tatcgattcg ctgaccgtta
    32761 aagataattt cgccgccctg cgattcgacc aggcgcagca acgcccgccc ggtagtggat
    32821 ttaccgctgc cagactcgcc caccagcgat agcgtttcgc caggccagag atcaaaactg
    32881 actttctcaa cggcatgcac ttcccgcgtt acgcgattca acaaaccgct gcgcaaaggg
    32941 aaacgggtga ccagattacg cacccgtaaa acaggttcgc catccaccac cgttttctgc
    33001 tcgatgggtg gttcctgttt tgctggatgt tcaagcgata tcaatgggaa acgtcgggga
    33061 taatctaacc ctttcatcgc accaagttgc ggaacagcag ctaacagcgc acgggtataa
    33121 ggatgttgcg gtgcatgaaa aatctgttcg acgctacccg tttccaccgc ctcgccctga
    33181 tacatcacca gtacccgatc ggcaatctct gccaccacgc ccatatcgtg agtgataaag
    33241 ataacgccca tcgacatctc tttttgcaat actttgatta attgcaggat ctgcgcctga
    33301 atagtgacat ccaacgcggt ggttggctca tcggcaatca gcaccgccgg gcggcatgac
    33361 agcgccatcg caatcatcac tcgctggcgc atcccgccag agagttgatg cggataacgt
    33421 gaaaggatgg tttgtgcctc cggaatgcgt acctgatcca gcatccgctt cgcctcgatc
    33481 atcgcttctt cacgactggc gttctgatgc agacgaattg attcggcaat ctgttcaccc
    33541 acagtaaata ccgggttcag cgatgtcatc ggttcctgaa atatcatcgc catatccgca
    33601 ccgcgcacat ggcgcatttg tgcagcgctc tgctcgctaa gttcaatcac ttcacgactg
    33661 cgccgccgca acagcatttg atcgcactgc actaaaccgc ccgcctgttc caacaggcgc
    33721 atcaatgcca gcgcagtcac tgacttaccg gagccggatt cgccaacaat tgccagcgtc
    33781 tcaccacgtt gcaggctaaa agagagattg cggaccgcag ctattttgtg ctgctcctgc
    33841 ataaaggcaa tattcagatt ttcaaccgcc agcacatcac cggcatcaag ttcatcactg
    33901 tgtggcaacg gtgtcccctt tttcacggta gataccggtg gttggcgtat cgcctgcgta
    33961 gccccaggcg cgatacattc cttcggtgtt aaacggtagc gcgacattcc cttcatggtc
    34021 gatagcgatt aagccaccgc taccgccaag cgcagggagt ttttccatta ctacccgctc
    34081 gcaggcttcc gcgagactta atccgccgta atccattaac gcggcgatat catatgccgc
    34141 cagcgcgcgg atgaagactt cgcccgtacc ggtacaggaa accgccacac tggcgttatt
    34201 ggcatagcat ccggccccca ctaagggact atcgccaact cgtccgggta atttattggt
    34261 cattccgccc gtggacgtgg ctgccgccag attgccgtct aaatccaacg ccacggcccc
    34321 cacggtgccc attttctgtt tttcatccag tggcgcaccg ctatggtcga gaaccattgc
    34381 cccttcctcg cgcgctgcca gtagttgttc ataacgcaaa ggcgtggaga aaatctccgg
    34441 cgagacgcgc tccatgccat gagcaaacgc aaaattttct gccccttcgc caatcatcat
    34501 cacatgcggg ctttgctcca tcaccagccg ggcggcaaga accggattac gcagatgact
    34561 aacgcccgcc accgcaccgg ctttcagggt gttaccatcc atcacacagg cgtccagttc
    34621 atgggtttca tcacgcgtaa agacagcgcc aattccggcg ttaaacagtg gacactcttc
    34681 cagcagacgc accgcttccg tcaccacatc cagcgcactt tcgcccgctt ccagcatttt
    34741 ctgcccggtt tcaacaatgg cagacaacgc ctcgatgtag cgtaattcct gttgcagact
    34801 catctgcgcg cggctaattg cacctgcgcc accatgaatt gcaatgactg ctttgcccat
    34861 agtattcgtc cattaagggt ggctcgccac ttttgctata aatatcagaa tttttatgaa
    34921 ttactcattc aatttttgaa tatagaaaga tggatatggc atgtaaaggc aggagccagc
    34981 gctacgcccg aggtggcata cagtctgtgg cgtttttctg acataatagg caaattcgat
    35041 tttgcctccg caggagtgtt ttcatggaat ttaccaccgg attgatgtcg ctcgacaccg
    35101 cgcttaatga gatgctttct cgcgttaccc cactgaccgc ccaggaaacg ctgccactgg
    35161 tacagtgttt tggtcgtatt ctggcgagcg atgtcgtttc gccactggat gtcccggggt
    35221 ttgataactc cgctatggac ggctacgcgg tgcgtttagc cgatattgcc tccgggcaac
    35281 cgctgcccgt tgccggtaaa tcctttgccg gtcagccgta ccatggtgaa tggcctgcgg
    35341 gtacctgcat tcgtattatg accggtgcgc cggtgccaga aggctgcgaa gcggtggtga
    35401 tgcaggagca gactgaacaa acggacaatg gcgtgcgttt tactgctgaa gtgcgtagcg
    35461 ggcaaaatat tcgccgtcgc ggtgaagata tatctgcagg tgcggttgtt ttcccggcgg
    35521 ggactcgcct gactaccgct gaactgccag tgattgcttc gctagggatt gccgaagttc
    35581 cggtgattcg taaagtgcgt gtagcgcttt tttctaccgg tgatgaactc cagttgccag
    35641 gccagccgct gggcgacggc caaatctacg ataccaaccg tctcgccgta cacctgatgt
    35701 tagaacagct gggatgcgag gtaatcaact tagggattat ccgcgacgat ccccatgccc
    35761 tgcgcgccgc ttttattgaa gccgacagcc aggcggatgt ggtgatcagt tccggcggtg
    35821 tttcagtggg tgaagcggat tacaccaaaa ccattcttga agagctgggg gagatcgcct
    35881 tctggaagct ggcgattaaa ccaggtaaac cgttcgcgtt cggaaaactt tctaatagct
    35941 ggttctgcgg cctgccgggc aacccggttt cagcgacgct gaccttctat caactggtac
    36001 agcctttgct ggcaaaacta agcggcaata ccgccagcgg cctgcccgcg cgccagcgtg
    36061 tgcgcacggc atcgcctctg aagaaatcgc caggacgcct tgatttccag cgcggcgtgc
    36121 tgcaacgcaa cgccgatggc gaactggaag tgacgaccac cggacatcag ggttcacata
    36181 tatttagctc ctttagcctc ggtaactgct ttatcgtgct ggagcgcgat cgcggcaatg
    36241 ttgaagcggg cgaatgggtg gaagtagaac cgtttaacgc gttgttcgga ggcctgtaat
    36301 ggcggaactc agcgatcagg agatgctgcg ctacaaccgg caaatcattc tgcgcggttt
    36361 tgattttgac ggtcaggagg cgctaaaaga ttctcgtgtg ctgatcgtcg gccttggtgg
    36421 actcggctgc gcagcttcgc agtatctggc aagcgccggt gtcggcaacc tgacgctgct
    36481 cgacttcgac acggtttcgc tctccaatct gcaacgccag acgctgcaca gtgatgccac
    36541 ggtcgggcaa ccaaaggtgg aatccgcccg tgacgccctg acgcggatca acccacatat
    36601 cgcgattacg ccagtcaatg cgctgcttga tgacgctgaa cttgctgcga tgattgcaaa
    36661 acacgatctg gtgctcgact gtacggataa cgttgcagta cgtaatcaac tgaacgcagg
    36721 ctgttttgcc gcgaaggtgc cgctggtttc cggcgcggca attcgtatgg aagggcaaat
    36781 caccgtcttc acttatcagg acggcgaacc gtgctatcgc tgccttagcc gtttgtttgg
    36841 cgagaacgcg ttaacctgcg tagaagcagg cgtgatggca ccgttgatcg gcgtaattgg
    36901 ttcgttgcag gcaatggaag cgatcaaact gctggcaggt tatggcaaac cagccagcgg
    36961 gaaaatcgtg atgtacgatg cgatgacctg tcagtttcgc gaaatgaaac tgatgcgcaa
    37021 tccggggtgt gaggtgtgcg ggcagtaatt gccacctcca ggcctgataa gacgtggcag
    37081 gcatcggtgc actccgccac gtcatcagca atttatgagg atgcagaacg tccggttaaa
    37141 tcgacgttct gccaaacgct ccctgccagt cctgctcaaa cttcgccaca gcggcatcaa
    37201 ccgccggata gctaatcatc tgttgtgcca catccagtgg cagagtaatt gattcacatc
    37261 ctgccagtaa gcagtccagc gcctgacgcg gggttttgaa acttgcggct aacacttttg
    37321 cctgcggcgc atgcattttc aataactggt gtaagtcggt cacagtctga atgccgctac
    37381 cgccctgagc atcaatacga ttaacgtaag gcgcaacata ttccgcacct gccagcgccg
    37441 acagtagccc ttgtgctgcg ccatataccg cggttcccag cgtcggaatc ccttccgctt
    37501 ttaacatctt aattgctgcc agcccctcag cggtcaccgg aactttcacc acgatatccg
    37561 caataataga acgcaattta cgtgcgtcat tcaccatccc ttcagcagtg gtagccatta
    37621 cctgggcaaa cagacgcccc tgaccgccca tcgcctcgtg aagttgcgga agcacaacct
    37681 ccagcggttt tttacccgcg gcgataatgc ttgggttagt ggttacaccc gccagcggaa
    37741 aaatacgtga cagcgccttc accgcaacaa cgtctgaagt atccagatac agttccataa
    37801 ccatcctcaa aatgtacttt ttgtctgaat gtagactatc acgggaaaac tctctgaaca
    37861 gttgacgcac atcaatctaa ctttcattcg aaagtatttt aatgtttgaa cgaaacagag
    37921 aggcaactat gattttcaat attcagcgct actcgaccca tgatggcccc ggtatccgca
    37981 cggtcgtatt tcttaaaggc tgttcgctgg gctgccgctg gtgtcagaac ccggaaagcc
    38041 gcgcccgcac gcaggatctg ctgtatgaca cacgactgtg tctggaaggc tgcgagctgt
    38101 gcgctaaggc cgcgccggaa gtgattgagc gcgcgctgaa tggcttgctg attcatcggg
    38161 aaaagttaac cccggagcat tttacggcgc taaccgactg ctgcccgaca caggcgttaa
    38221 ccgtgtgtgg tgaagtgaaa agcgttgagg agatcatggc gaccgttctg cgcgataaac
    38281 cgttttacga tcgcagcggc ggcggcttaa cactttctgg cggtgaacca ttcatgcagc
    38341 cggaaatggc gacagcgcta ctgcaagcca gccacgaggc cggcattcat actgcggtag
    38401 aaacctgtct gcatgtgccg tggaaatata tcgccccttc tctgccctat atcgatctgt
    38461 ttcttgccga tttaaaacac gttgccgacg cgccgtttaa acagtggacc gacggtaacg
    38521 ccgccagagt gctggataac ctgaaaaaac tcgccgcagc gggcaaaaaa atcattatcc
    38581 gcgtgccgct gattcagggc tttaatgccg acgaaacctc tgtgaaagcc attaccgatt
    38641 ttgccgccga cgagctgcac gttggtgaaa ttcatttcct gccctaccac acgctgggca
    38701 tcaacaaata tcacttactt aatctgccct atgacgcccc ggaaaaaccg cttgatgcgc
    38761 cagaactgct cgactttgcc cagcagtatg cctgccagaa agggttaacc gcgaccttac
    38821 gaggataaca accatgacca cactgaaact ggacacgctc agcgaccgca ttaaagcgca
    38881 caaaaatgcg ctggtgcata ttgtgaaacc gccggtttgt accgagcgcg cgcagcacta
    38941 taccgagatg tatcaacaac atctcgataa gccgatccct gtacgtcgcg cgctggcgct
    39001 ggcacatcac ctggcgaatc gcactatctg gatcaagcac gatgagttga tcatcggcaa
    39061 ccaggcaagc gaagttcgcg ccgcgccgat cttcccggaa tatactgtct cgtggatcga
    39121 aaaagagatt gatgatctgg cagatcgtcc cggtgctggc tttgcggtga gcgaagagaa
    39181 caaacgcgtt ctgcatgaag tgtgcccgtg gtggcgcggt cagaccgtac aggatcgctg
    39241 ctacggcatg tttaccgatg agcaaaaagg tctgctggcg accggcatca ttaaagcgga
    39301 aggcaatatg acctccggcg atgcgcacct ggcggtcaat ttcccgctgc tgctggaaaa
    39361 agggcttgat ggtctgcgcg agaaagtagc ggaacgtcgc tcgcgcatca acctgacggt
    39421 gctggaagat ttacacggtg agcaattcct gaaagcgatt gatatcgtgc tggtggcagt
    39481 cagtgaacac attgaacgtt tcgctgccct ggcgcgtgaa atggccgcga ccgaaacccg
    39541 cgaaagccgt cgcgatgaac tgctggcgat ggcagaaaac tgcgatctta tcgcccacca
    39601 accgccgcag actttctggc aggcgctgca actgtgttac ttcatccagt tgattttgca
    39661 gatcgaatct aacggtcact cggtatcgtt tggtcgtatg gaccagtatc tctacccgta
    39721 ctatcgccgt gacgttgaac tgaaccagac actggatcgc gaacacgcca tcgagatgct
    39781 gcatagctgc tggttgaaat tgctggaagt gaacaaaatc cgctccggct cacactcaaa
    39841 agcctctgcg ggaagtccgc tgtatcagaa cgtcaccatt ggcgggcaaa atctggttga
    39901 tggtcaaccg atggacgcgg taaatccact ctcttatgcg atcctcgaat cttgcggtcg
    39961 cctgcgttcg actcagccta acctcagcgt gcgttaccat gcgggtatga gtaacgattt
    40021 cctcgacgcc tgtgttcagg tgatccgctg cggcttcggg atgccggcgt tcaacaacga
    40081 cgaaatcgtc atcccggaat ttattaaact cggtattgaa ccgcaggacg cttacgacta
    40141 cgcagcgatt ggttgtatcg aaaccgccgt cggtggcaaa tggggctatc gctgtaccgg
    40201 catgagcttt atcaacttcg cccgcgtgat gctggcggcg ctggaaggcg gtcgtgatgc
    40261 caccagcggt aaagtgttcc tgccacaaga aaaagcgttg tcagcgggta acttcaacaa
    40321 cttcgatgaa gtaatggacg catgggatac gcaaatccgt tactacacgc gtaaatcaat
    40381 cgaaatcgaa tatgtcgtcg acaccatgct ggaagagaac gtacacgata ttctctgctc
    40441 ggcgctggtg gatgactgta ttgagcgagc gaaaagtatc aagcaaggcg gcgcgaaata
    40501 tgactgggtt tctggcctgc aggtcggcat tgctaacctc ggcaacagcc tggcggcagt
    40561 gaagaaactg gtatttgaac agggcgcgat tggtcagcaa cagctggctg ccgcactggc
    40621 ggatgacttc gacggcctga ctcacgagca gctgcgtcag cggctgatta acggtgcgcc
    40681 taagtacggc aacgacgatg atactgtcga tacgctgctg gctcgcgctt atcagaccta
    40741 tatcgacgaa ctaaaacagt accataaccc gcgctatggt cgtggtccgg ttggcggcaa
    40801 ttattacgcg ggtacgtcgt ctatctccgc taacgtaccc tttggcgcgc agacgatggc
    40861 aacgccggac ggacgtaaag cgcatacccc gctggcagaa ggcgctagtc cggcctccgg
    40921 tactgatcat cttggaccta ctgcggtcat tggctcggtg ggtaaactgc ctacggcagc
    40981 gattctcggc ggcgtgctgc tcaaccagaa actgaatcca gcaacgctgg agaacgaatc
    41041 tgacaagcag aaactgatga tactgctacg caccttcttt gaagtgcata aaggctggca
    41101 tattcagtac aacatcgttt cccgcgaaac gctgctggaa gcgaaaaaac atccggatca
    41161 gtaccgcgat ctggtagtgc gtgtcgcagg ctattccgcc ttcttcaccg cgctctctcc
    41221 agacgctcag gacgatatca tcgcccgtac tgaacatatg ctgtaatctc ctcagcccgg
    41281 cggcgtcgtc gccgggctaa atcattcaca tcatcaattt cagcctcgct ttcattcgaa
    41341 atataatttg tgctctgcgt cacattgtta ttacactgtt acggtttcat tttcgagcca
    41401 ggagcacacc tatgagcgta aaagttatcg tcacagacat ggacggtact tttcttaacg
    41461 acgccaaaac gtacaaccaa ccacgtttta tggcgcaata tcaggaactg aaaaagcgcg
    41521 gcattgagtt cgttgtcgcc agcggtaatc agtattacca gcttatttcg ttttttcctg
    41581 agctaaagga tgagatctct tttgtcgcgg aaaacggcgc actggtttac gaacatggca
    41641 agcaactgtt ccacggcgaa ctgacccgac atgaatcgcg gattgttatt ggcgagttgc
    41701 taaaagataa gcaactcaat tttgtcgcct gcggtcagca aagtgcatat gtcagcgaaa
    41761 acgcccccga agcattcgtc gcactgatgg caaaacacta ccatcgcctg aaacctgtaa
    41821 aagattatca ggagattgac gacgtactgt tcaagttttc gctcaacctg ccggatgaac
    41881 aaatcccgtt agtgatcgac aaactacaca tagcgctgga tggcattatg aaacccgtta
    41941 ccagtggttt tggctttatc gacctgatta ttcccggtct acataaagca aacggtattt
    42001 cgcggttact gaaacgctgg gatctgtcgc cgcaaaatgt ggtagcgatt ggcgacagcg
    42061 gtaacgatgc ggagatgctg aaaatggcgc gttattcctt tgcgatgggc aatgctgcgg
    42121 aaaacattaa acaaatcgcc cgttacgcta ccgatgataa taatcatgaa ggcgcgctga
    42181 atgtgattca ggcggtgctg gataatatcg ctcctttcaa ttgttgatct tccccctggc
    42241 cgaagtgacc acttcggcct ctctcctttt tgctcgcttt gtgcactgac tcaagatttt
    42301 tatcttgcgt taacttattt ttaacttaat gtatcaatcg caatcatttt actttcgaat
    42361 gaaagaagcg aggttgatat gactttttcc tgtgagatgc tgcccacaga tgcgaaagca
    42421 gctatccgag agatgaaagc tgcgctgcgg gaacagctca gtgatgtgca acaggttttc
    42481 gatacactct ctgcaaaaat cgaaacgcgt gttgctgaaa tcgatgccct aaaagcgcaa
    42541 gggctgccgg tttggccgga agtttcttat agtgatattg ccgcaggtac agtaagcgat
    42601 gccactcgca atgaaattaa acgtcgcggt tgtgcagtca taaaaggcca tttccctcgc
    42661 gagcaggcga tggcgtggga tcgtgccatg ctcgattatc ttgatatcaa tcattttgat
    42721 gatgtttata aaggccctgg cgacagtttt ttcggctcgc tggaggcttc tcgtcccgaa
    42781 atctatccca tttactggtc gcaagcacag atgcaggcgc ggcagagcga aaacatggct
    42841 gctgtgcaat cttttctaaa ccgactgtgg aagtctgaaa gcaacggcaa acagtggttt
    42901 aatcctgata tcagtgtgat ttatccagac aggatccgcc gccgccctcc aggtactaca
    42961 tctaaaggcc tgggcgcgca taccgattct ggtgcgctgg aacggtggtt attacccgct
    43021 tatcagcgcg tttttgccaa tgtgtttaat ggtaatttcg atgattacga tccctgggat
    43081 gctgcacacc ggaccgatgt cgaagagtac acggtagata acactacaaa atgttcggtg
    43141 ttccgcacgt ttcagggctg gacggcgctc tctgatatgt tatcgggtca gggattgtta
    43201 catgtggtgc ctatcccgga agcgatggcg tatgttttat tgcgcccgct gctggatgac
    43261 gtaccggacg atgaactgtg cggggttgca cctggcaaag ttctgcccat ttcagagaaa
    43321 tggcatccac tactgcttaa agcgctgact tccattcccg cgataacagc aggggattcc
    43381 gtctggtggc actgcgatgt gattcactcc gtcgcgccag tagacaatca acagggttgg
    43441 ggcaacgtga tgtacattcc tgcggcaccg atgtgcgaga aaaatcgggc ttatgcacac
    43501 aaagtcaaaa tggcgctgga aaaaggcgca tcacctggcg acttcccgcg ggaagattat
    43561 gaagcaaact gggaaggccg ctttacgctg gaggatctga acattcacgg taagcgcgca
    43621 ctgggcatgg atgtttgaga gttaatggaa gtgaattgat gtgggattaa gaaaatcgat
    43681 tcaacgttac tcagattgct gacaaatgcg ctttgttaat gcctgatgcg gcgttaacgc
    43741 cttatccggc ctacaaagtc ctgcagattc aatatattgc aggtactgcg taggcctgat
    43801 aagcatagcg catcaggcaa tcttgcgttt gtcatcagcc gccgtcaccc cgacagtatc
    43861 gggctgacgg ataataactt actcgatccc tttactacgc agataatctt cgtaattacc
    43921 gctaaagtcg atcacgcgtt ccggggtgat ttccagaatt cgggtcgcca gggagcttac
    43981 aaactcacgg tcgtgagaca caaagatcag cgtgccctga tacagttcca gcgccatgtt
    44041 cagcgactca atggattcca tatccaggtg gttggtcggt tcgtccatga tcagaatgtt
    44101 cggcttctgc atcattaact taccaaacag catccgccct ttctccccac cggaaagcac
    44161 tttcgctggc tttttgatgt cgtcctggct gaacagtaaa cgaccgagaa tgctgcgtac
    44221 cgcctgctcg tcatcgcctt cctgcttcca ctggctcatc cattcgaaca cggtcagatc
    44281 attttcgaac tcatattcat ggtcctgggc gtagtaacca atgcgcgcgt tctcagacca
    44341 ttttacggtg ccgctgtccg gttgcagatc gcccaccagc gttttcagca gcgttgattt
    44401 accgacgccg ttggtaccca gtacagccag tttttcaccc acttccagca gcaggttgag
    44461 atttttaaac agtggaccgt tatcaaaccc tttagtcaga ccttccactt ccagcgcgtt
    44521 acggaacagt ttcttatcct gttcaaaacg gatgaacggg ttctgacggc tggaggcttt
    44581 cacctcttcc agtttgattt tatcaatctg gcgcgcgcgc gaagttgcct ggcgagattt
    44641 cgaggcgttg gcgctaaagc ggctaacgaa agattgcaac tcagcaatct gcgctttctt
    44701 cttggcgtta tcagccagca gacgttcacg cgcctgagtc gccgccgtca tgtactcatc
    44761 gtagttgccc ggataaacgc gcagctcgcc gtaatccaga tccgccatgt gggtacaaac
    44821 catgttaagg aagtgacggt cgtgcgagat gatgatcatg gtgctgtcac gctcgttcag
    44881 cacctgttcc agccagcgaa tggtgtcgat gtcgaggttg ttggtcggtt cgtcgagcag
    44941 gagaatgtcc ggatcagcaa acagcgcctg cgccagcagc acacgcagct tccagccagg
    45001 agcaacttca ctcatcgggc cgtagtgctg ttccactgga attcccacgc caagcaacag
    45061 ttcaccagcg cgagcttccg cagagtaacc gtccagttcg ccgtatttca cttccagatc
    45121 ggccacttta tagccgtctt cttcactcat ttccggcaaa gcatagatgc ggtcacgctc
    45181 ctgcttcact tcccacaact ctttatgccc catgatcacc gtatccagca cagtgaactc
    45241 ttcaaaggca aactgatcct gacgcagctt accaatgcgc tcgttgggat cgagggaaac
    45301 gttacccaac gtcggctcta agtcgccgcc gaggatcttc ataaaggtgg atttaccact
    45361 accgttcgcg ccaatcaggc cgtaacggtt gccgccgcca aatttgacgg aaatgttttc
    45421 aaacaacggc ttactgccga actgcatggt gacgttactg gaaactaaca caggcgtatc
    45481 ctgaaaagag atatgacaaa ccgcgcatta tgccataaat ctgaaagaag atcctgctcc
    45541 tcgcttaagg tctacactca agccaaaggc aaaaaatgtg atttcgtaca catctgattt
    45601 cactgtgagc tggaatgaac ttataatgcg cttccaatac tctaatattc tcaacccaat
    45661 ggcctgccag gcacaaaatc tcgcttaaca tgaatatgaa attgaaaaca ttattcgcag
    45721 cggccttcgc tgttgtcggc ttttgcagta ccgcctctgc ggtaacttat cctctgccaa
    45781 ccgacgggag tcgcctggtt ggtcagaatc aggtgatcac cattcctgaa ggtaacactc
    45841 agccgctgga gtattttgcc gccgagtacc agatgggact ttccaatatg atggaagcga
    45901 acccgggtgt ggataccttc ctgccgaaag gcggtactgt actgaacatt ccgcagcagc
    45961 tgatcctgcc ggataccgtt catgaaggca tcgtcattaa cagtgcagag atgcgtctgt
    46021 attactatcc gaaaggaacc aacaccgtta tcgtgctgcc gatcggcatt ggtcagttag
    46081 gcaaagacac gcctatcaac tggaccacca aagttgagcg taaaaaagca ggcccgacct
    46141 ggacgccgac cgccaaaatg cacgcagagt accgcgctgc gggcgaaccg cttccggctg
    46201 tcgttccggc aggtccggat aacccgatgg gtctgtatgc actctacatc ggtcgcctgt
    46261 acgctatcca tggcaccaac gccaacttcg gtatcggcct gcgtgtaagt catggctgtg
    46321 tgcgtctgcg taacgaagac attaaattcc tgttcgagaa agtgcctgtc ggtactcgcg
    46381 tgcagtttat tgatgagccg gtgaaagcga ctaccgaacc agacggcagc cgttacattg
    46441 aagtccataa cccgctgtct accaccgaag cccagtttga aggtcaggaa attgtgccaa
    46501 ttaccctgac gaagagcgtg cagacagtga ccggtcagcc agatgttgac caggttgttc
    46561 ttgatgaagc gatcaaaaat cgttccggga tgccggttcg tctgaattaa tcgtcaaaac
    46621 gtaaaacaga ggcggattca taatccgcct tttttatttg gccagaccct agttggctgg
    46681 gagtataacc aacaaaacat atcccaccag cgccgcccac aacagcatcg gtattgaata
    46741 gagatggaaa cgccaccaga tacggcgatc gttcgccatc cgtaacgcaa tcaaatttgc
    46801 cagcgatccg ggtaataaac caaagccccc tacgtttacc gcccatgcca gcagcaacga
    46861 cggcggcaca tagttcagca acaatatggt actcggcacg ttactgatca cctgcgataa
    46921 accgattgcc gttaaccata atccgggttc ggataaatga ctcacgttac ttaacacccc
    46981 ctgcaaagct ggcagttggg ttagtaaatg gacgtcgata aacatcgcca taaacactag
    47041 cagcagcgtc cagtccacac tgagcaccac gcgacgtgcc agcagcgtaa agcccaccgc
    47101 cacaatcacc agtccccaca gctcttgttt taactccagc gccatcagaa agacgatata
    47161 cagccccaga caactccaca ccagccgcgg tttccactcc ggcgtttgcg cccccgtatg
    47221 gtattgcagc gcctttccag ggaaacaaca ccagcacaac agcaggagtg tcagcatcat
    47281 tgcgccagcc agcggtgcca tttgggcaat aaatccggca aacgaaagac cagaacgtcc
    47341 ccagataaga atattttgcg ggttgccaat tggcgtcagt agcgaaccag cgttgactgc
    47401 cagcgcctca aaaataatca gccgattaac cgggatctca cacagtcttt ttagcgtgat
    47461 agtcagcgga acaacaataa acagcgcgac atcgttggtc agaaaggtag aaagcagcgc
    47521 cgccgccagc accataaaca tcgccagccg acgctccgta gcaaagcggc gcaccatttt
    47581 gcgccccagc acatcaaaat aaccgcttaa ctccacacct ttggtcagca gcatcaggcc
    47641 gcttaaggtg atgatggtgt gccagtcgat agcagcaggc caggatttcg gtgcaaaggg
    47701 cacgaaaaag cttaatccga taccaacaag aattaataac tgaaaaaaac gatcgccttg
    47761 cagcgtgcgt aaaaaaggca ggctcatttg gcaccgtgtt tctgggtaaa caaacgaaag
    47821 gcatccagcg tctcttcact aacatggtgc tccatgcctt ccgcgtcgcg acgggcgatt
    47881 tccggactga cgcccaacac caacaagaaa ttttcgacta tctgatgtcg ctcgcggctt
    47941 tcctgcgcca gcttctctcc ttctgccgtt aaaaacacgc ctcgccaggg gatcatttca
    48001 atcagcccca tggttgccag ccgcttaagc attttagcca ccgtcggttg cgaaactccc
    48061 agacgagcag ccatgtccac ctgacgagct tcccccactt ccctgatcaa gtcagaaatc
    48121 agctcaacgt aatcatcaat aagctcgcgc cgatgcgcct ctctgacctg gcggaaccct
    48181 tcaacgtgct cttccacgtt cactaactgc gtcacttttt ttgctgttgg cgtacctgcg
    48241 cgacgactca ttgtgcttcc tcattatggt gacgcataca tagcgttctc atttcgtgcg
    48301 cacattgtaa accagagttg cgaaggtaca aaaaattaac gttttagcaa tagctatata
    48361 atatagcctg tgctatatct gtatgtaatg caatcatccc tcaaggatcg acgggattag
    48421 caagtcagga ggtcttatga atgagttcaa gaggtgtatg cgcgtgttta gtcattctcc
    48481 ctttaaagta cggttaatgc tgctctctat gttgtgcgat atggtcaaca acaaaccgca
    48541 gcaagataaa ccttcagata aatagcggcg tcgcggtacg ccgcttcact cctgctttta
    48601 tgcaggcata acgcgttttg gtctgaaaaa ccccactttt tgtcggattt gcaatcccct
    48661 tcgcaaaaga tttgttcgtc agtagttgac ctgaacggcg gctcgctcta tcttcttgca
    48721 gccctgcgta tattgcggct cgcggatgcg gccccctttc ccctcttcac gcactcttgc
    48781 aggtattgac ccttgacgcc agggtaagca catggcgttt gttacgatag tggcatatga
    48841 atttaaccct caaagaatcg cttgttaccc gtagccgggt atttagcccg tggactgcgt
    48901 tctacttttt acagtcgcta ttaattaacc tcggcttagg ttaccccttc agtttgctct
    48961 acaccgctgc gtttacggct attttgcttt tgctatggcg aacattgcct cacgtacaaa
    49021 aagttctggt cggtgtcagt tcgctggtgg cggcttgtta tttccctttt gctcaggcct
    49081 acggcgcacc taatttcaat acattgctgg cattgcactc caccaatatg gaagagtcga
    49141 ccgaaatcct gacgattttt ccgtggtaca gctacctggt cggcttattt atttttgcgc
    49201 tcggcgtaat agcaatcagg cgaaaaaaag agaatgaaaa agcgcactgg aataccttcg
    49261 acagcctgtg tctggtattc agtgtggcga cattttttgt tgctcccgtg caaaacctgg
    49321 cctggggcgg cgtatttaaa ctgaaagata ctggctatcc ggtatttcgt tttgctaagg
    49381 atgtcatcgt caataataac gaggtgattg aagagcaaga acggatggca aaactgtccg
    49441 gaatgaaaga tacctggacg gtcactgccg ttaagccgaa gtatcagacc tatgtggtgg
    49501 tgatcggtga aagcgcgcgt cgcgatgccc tcggtgcctt tggcggtcac tgggacaata
    49561 ccccgtttgc cagcagcgtt aacggtttga tatttgctga ctacattgcc gccagtggct
    49621 ccacgcagaa atcgcttggc ttaacgctca atcgcgtagt cgatggcaaa ccacagtttc
    49681 aggataactt tgtcaccctg gcaaatcgcg cgggcttcca gacctggtgg ttttccaacc
    49741 agggtcaaat cggcgaatac gataccgcca tcgccagcat cgccaaacga gcagatgaag
    49801 tgtacttcct gaaagaaggt aattttgaag cagataaaaa cacgaaagac gaagcgttac
    49861 tggatatgac cgctcaagtg ctggcgcaag agcactcgca accgcagctg attgttctac
    49921 atctgatggg ctcgcatccg caggcctgcg accggacaca aggcaaatac gaaacctttg
    49981 tgcaatcaaa agaaacgtcg tgctatctct ataccatgac gcaaacagac aatttactgc
    50041 gtaagctgta cgatcagtta cgcaacagcg gcagtagctt ctcgctggtt tacttttccg
    50101 accacggtct ggcctttaaa gagcgcggca aagacgtgca ataccttgcc catgatgata
    50161 agtatcagca aaatttccag gtgcctttta tggtcatttc cagcgacgat aaagcgcatc
    50221 gggtgattaa agcccgccgc tcggccaatg acttcttagg ctttttctcc cagtggacgg
    50281 gaattaaagc gaaggaaata aatatcaaat acccgtttat atctgagaag aaagccgggc
    50341 cgatatacat caccaacttc cagttacaga aggtggatta caaccatctc ggaaccgata
    50401 ttttcgaccc gaaaccttaa aaacaaaaat ccgccccgag aggcggattt tttatatcac
    50461 cgaagtgatt agaagcggta accaacaccg gcaatccagg tgcctacgtc aacgctacga
    50521 atacggctct gctcgtaaga gaagtccaga gcaacgtttt ccatcgggtt gaactgcaga
    50581 ccagcaccgt aggagaaacc gtagtcgctg gtgtcgtgtt tgtaggtcgg gtattcagtg
    50641 gtctggaatt taccataacc cacacccact acaccgtaga tgcttgccca gtcgttaatg
    50701 cggtaagccg gaccagcagt gatgccgtag tactggtttt tgttgtagtc accagagctt
    50761 gcagtacggc ttttctcggt gtaagtgaaa gaaccgatca cacccagcgg gctgttgtct
    50821 tcttcatagc ggtatttcag gttgaaaccg cccattttgt tcatttggcc ctgagcgtcg
    50881 ctctgtgcgt aaccgccagt tacagtagaa gtcgcagcta cggaagtacc tgcggtgaaa
    50941 gccagaactg cggccagtgc tgaaagacat gcaatttttt tcataaccac ctcaaatgtg
    51001 attcaaataa gtcctaagtt ttaaatatat caaaaattaa tgggaaactc ttcgcgattt
    51061 gtgatgtcta acgggccatt tcatgtaaca gaacgtttcc atacaccgct atccatctaa
    51121 atttaaatca ctttttcaga gaactgcata agtattacgc atgttttccc tgtcattcat
    51181 ccagattatt cctaatcacc agactaatga ttccatcaat cctggcgcat tttagtcaaa
    51241 acgggggaaa attttttcaa caaatgctca accagcattg ggtatatcca gtacactcca
    51301 cgctttactt aggtttagat atttgtggga gaaaggatgc ctggttcatt acgtaaaatg
    51361 ccggtctggt taccaatagt catattgctc gttgccatgg cgtctattca gggtggagcc
    51421 tcgttagcta agtcactttt tcctctggtg ggcgcaccgg gtgtcactgc gctgcgtctg
    51481 gcattaggaa cgctgatcct catcgcgttc tttaagccat ggcgactgcg ctttgccaaa
    51541 gagcagcggt taccgctgtt gttttacggc gtttcgctgg gtgggatgaa ttatcttttt
    51601 tatctttcta ttcagacggt accgctgggt attgcggtgg cgctggagtt caccggacca
    51661 ctggcggtgg cgctgttctc ttctcgtcgc ccggtagatt tcgtctgggt tgtgctggcg
    51721 gttcttggtc tgtggttcct gctaccgctg gggcaagacg tttcccatgt cgatttaacc
    51781 ggctgtgcgc tggctctggg ggccggggct tgttgggcta tttacatttt aagtgggcaa
    51841 cgcgcaggag cggaacatgg ccctgcgacg gtggcaattg gttcgttgat tgcagcgtta
    51901 attttcgtgc caattggagc gcttcaggct ggtgaagcac tctggcactg gtcggttatt
    51961 ccattgggtc tggcggtcgc tattctctcg accgctctgc cttattcgct ggaaatgatt
    52021 gccctcaccc gtttgccaac gcgcacattt ggtacgctga tgagcatgga accggcgctg
    52081 gctgccgttt ccgggatgat tttcctcgga gaaacactga cacccataca gctgctggcg
    52141 ctcggcgcta tcatcgccgc ttcaatgggt tctacgctga cagtacgcaa agagagcaaa
    52201 ataaaagaat tagacatta
//
LOCUS       DAPHCV010000015        51955 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.015, whole genome shotgun sequence.
ACCESSION   DAPHCV010000015 DAPHCV010000000
VERSION     DAPHCV010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.015"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(39..1679)
                     /gene="pgm"
                     /locus_tag="RH033_000902"
     CDS             complement(39..1679)
                     /gene="pgm"
                     /locus_tag="RH033_000902"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HDV2990132.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDIIVQENNGFTPTPAISNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(1705..2250)
                     /gene="seqA"
                     /locus_tag="RH033_000903"
     CDS             complement(1705..2250)
                     /gene="seqA"
                     /locus_tag="RH033_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HDV2990133.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            2435..3199
                     /gene="ybfF"
                     /locus_tag="RH033_000904"
     CDS             2435..3199
                     /gene="ybfF"
                     /locus_tag="RH033_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDV2990134.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            3339..3632
                     /gene="ybfE"
                     /locus_tag="RH033_000905"
     CDS             3339..3632
                     /gene="ybfE"
                     /locus_tag="RH033_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HDV2990135.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            3772..4302
                     /gene="fldA"
                     /locus_tag="RH033_000906"
     CDS             3772..4302
                     /gene="fldA"
                     /locus_tag="RH033_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HDV2990136.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            4512..4598
                     /gene="uof"
                     /locus_tag="RH033_000907"
     CDS             4512..4598
                     /gene="uof"
                     /locus_tag="RH033_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HDV2990137.1"
                     /translation="MIRIISRANSVTSPNEVNRLVTGQIPHD"
     gene            4591..5037
                     /gene="fur"
                     /locus_tag="RH033_000908"
     CDS             4591..5037
                     /gene="fur"
                     /locus_tag="RH033_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HDV2990138.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(5121..5447)
                     /locus_tag="RH033_000909"
     CDS             complement(5121..5447)
                     /locus_tag="RH033_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2990139.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFAEQESVRVLDYQQCIQATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(5497..6903)
                     /gene="chiP"
                     /locus_tag="RH033_000910"
     CDS             complement(5497..6903)
                     /gene="chiP"
                     /locus_tag="RH033_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HDV2990140.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAIPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAVFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSINDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            7162..7320
                     /locus_tag="RH033_000911"
     CDS             7162..7320
                     /locus_tag="RH033_000911"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2990141.1"
                     /translation="MKAVTFYLIMFCDFKFILWINCEAELIREEKQKKRRRARRLGLF
                     ACFLPDAA"
     gene            complement(7351..9015)
                     /gene="glnS"
                     /locus_tag="RH033_000912"
     CDS             complement(7351..9015)
                     /gene="glnS"
                     /locus_tag="RH033_000912"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HDV2990142.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGSFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(9218..11164)
                     /gene="nagE"
                     /locus_tag="RH033_000913"
     CDS             complement(9218..11164)
                     /gene="nagE"
                     /locus_tag="RH033_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HDV2990143.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATSFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVVDSARVNDVMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAETAPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            11497..12297
                     /gene="nagB"
                     /locus_tag="RH033_000914"
     CDS             11497..12297
                     /gene="nagB"
                     /locus_tag="RH033_000914"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDV2990144.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            12357..13505
                     /gene="nagA"
                     /locus_tag="RH033_000915"
     CDS             12357..13505
                     /gene="nagA"
                     /locus_tag="RH033_000915"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDV2990145.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITRTIVNGNEVVTQ"
     gene            13514..14734
                     /gene="nagC"
                     /locus_tag="RH033_000916"
                     /gene_synonym="nagR"
     CDS             13514..14734
                     /gene="nagC"
                     /locus_tag="RH033_000916"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HDV2990146.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            14782..15534
                     /gene="nagD"
                     /locus_tag="RH033_000917"
     CDS             14782..15534
                     /gene="nagD"
                     /locus_tag="RH033_000917"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HDV2990147.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            15931..17595
                     /gene="asnB"
                     /locus_tag="RH033_000918"
     CDS             15931..17595
                     /gene="asnB"
                     /locus_tag="RH033_000918"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HDV2990148.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALEE
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            complement(17637..18074)
                     /locus_tag="RH033_000919"
     CDS             complement(17637..18074)
                     /locus_tag="RH033_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2990149.1"
                     /translation="MLRFMTALMGALLLMQSAFADTVKPEIGKYVFGYRGQEGAVVWM
                     MRIGPKASNEALIQVSHVDNDIDGQIFRCKVKALQEGEKSYTAVIKGKSFELLRLKEG
                     NGSLHIPNEQATWSVAYSDELSNSDVANPEHFLTAYQNQQAPQ"
     gene            18406..18482
                     /locus_tag="RH033_000920"
     tRNA            18406..18482
                     /locus_tag="RH033_000920"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18440..18442,aa:Met,seq:cat)
     gene            18492..18576
                     /locus_tag="RH033_000921"
     tRNA            18492..18576
                     /locus_tag="RH033_000921"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18526..18528,aa:Leu,seq:tag)
     gene            18600..18674
                     /locus_tag="RH033_000922"
     tRNA            18600..18674
                     /locus_tag="RH033_000922"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18632..18634,aa:Gln,seq:ttg)
     gene            18709..18783
                     /locus_tag="RH033_000923"
     tRNA            18709..18783
                     /locus_tag="RH033_000923"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18741..18743,aa:Gln,seq:ttg)
     gene            18799..18875
                     /locus_tag="RH033_000924"
     tRNA            18799..18875
                     /locus_tag="RH033_000924"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18833..18835,aa:Met,seq:cat)
     gene            18924..18998
                     /locus_tag="RH033_000925"
     tRNA            18924..18998
                     /locus_tag="RH033_000925"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18956..18958,aa:Gln,seq:ctg)
     gene            complement(19152..20327)
                     /gene="ubiF"
                     /locus_tag="RH033_000926"
     CDS             complement(19152..20327)
                     /gene="ubiF"
                     /locus_tag="RH033_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HDV2990150.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVMVIEHAQPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRHNDLQELELKGGETIRAKL
                     VIGADGANSQVRQIAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQTEIAKHFPSCLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPILKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            20473..21897
                     /gene="miaB"
                     /locus_tag="RH033_000927"
     CDS             20473..21897
                     /gene="miaB"
                     /locus_tag="RH033_000927"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDV2990151.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            22050..23090
                     /locus_tag="RH033_000928"
     CDS             22050..23090
                     /locus_tag="RH033_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDV2990152.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            23087..23554
                     /gene="ybeY"
                     /locus_tag="RH033_000929"
     CDS             23087..23554
                     /gene="ybeY"
                     /locus_tag="RH033_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDV2990153.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEAIETEIMLALGYEDPYIAEKE"
     gene            23644..24522
                     /gene="corC"
                     /locus_tag="RH033_000930"
     CDS             23644..24522
                     /gene="corC"
                     /locus_tag="RH033_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HDV2990154.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            24547..26085
                     /gene="lnt"
                     /locus_tag="RH033_000931"
     CDS             24547..26085
                     /gene="lnt"
                     /locus_tag="RH033_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HDV2990155.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSLNGIQLTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKH"
     gene            complement(26163..28040)
                     /locus_tag="RH033_000932"
     CDS             complement(26163..28040)
                     /locus_tag="RH033_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDV2990156.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCVGIFCYLAQWMSYEEVDQSA
                     LIHLGANVASLTLSGESWRLLSSVFLHSSVSHLLMNMFALLVVGGAVERILGKWRLLI
                     IWLFSGIFGGLISACYALHESEQIVISVGASGAIMGIAGAAIATQLSSGAGTHHKNQR
                     RVFSLLGMVALTLLYGARQAGIDNACHIGGLIAGGALGWLSARLVGQNRLVTEGGIIV
                     AVTLLLTGTIWFVQQQIDESVLQVRQSLREAFYPQEIEQERRQKKQQLVEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNTLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSLDEKLIYATSFEANAVSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVTDIPLEKYDGTGTSGKPGAWV
                     MALSPDEKTLLIPGMVRGDIVRINTITHQKEDFPTGDARGIVSAMGFRPKNGEIIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNRLIQMDRTRSLDPQAIIRHPQYGDVACIPE
                     P"
     gene            28495..29403
                     /gene="gltI"
                     /locus_tag="RH033_000933"
     CDS             28495..29403
                     /gene="gltI"
                     /locus_tag="RH033_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HDV2990157.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNAERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            29573..30313
                     /gene="gltJ"
                     /locus_tag="RH033_000934"
     CDS             29573..30313
                     /gene="gltJ"
                     /locus_tag="RH033_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HDV2990158.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            30313..30987
                     /gene="gltK"
                     /locus_tag="RH033_000935"
     CDS             30313..30987
                     /gene="gltK"
                     /locus_tag="RH033_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HDV2990159.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            30987..31712
                     /gene="gltL"
                     /locus_tag="RH033_000936"
     CDS             30987..31712
                     /gene="gltL"
                     /locus_tag="RH033_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HDV2990160.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDQAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            31830..32765
                     /gene="rihA"
                     /locus_tag="RH033_000937"
     CDS             31830..32765
                     /gene="rihA"
                     /locus_tag="RH033_000937"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HDV2990161.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRNLIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLCESEEPITIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(32810..33292)
                     /locus_tag="RH033_000938"
     CDS             complement(32810..33292)
                     /locus_tag="RH033_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HDV2990162.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            33527..36109
                     /gene="leuS"
                     /locus_tag="RH033_000939"
     CDS             33527..36109
                     /gene="leuS"
                     /locus_tag="RH033_000939"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDV2990163.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            36124..36705
                     /gene="lptE"
                     /locus_tag="RH033_000940"
     CDS             36124..36705
                     /gene="lptE"
                     /locus_tag="RH033_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HDV2990164.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            36705..37736
                     /gene="holA"
                     /locus_tag="RH033_000941"
     CDS             36705..37736
                     /gene="holA"
                     /locus_tag="RH033_000941"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDV2990165.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAIRQVAAV
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHMPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLARTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            37738..38379
                     /gene="nadD"
                     /locus_tag="RH033_000942"
     CDS             37738..38379
                     /gene="nadD"
                     /locus_tag="RH033_000942"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDV2990166.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            38403..39014
                     /locus_tag="RH033_000943"
     CDS             38403..39014
                     /locus_tag="RH033_000943"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HDV2990167.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHG
                     VSFDLVLCSELERAQHTARRVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARL
                     IGMPAEAMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            39274..39591
                     /gene="rsfS"
                     /locus_tag="RH033_000944"
                     /gene_synonym="rsfA"
     CDS             39274..39591
                     /gene="rsfS"
                     /locus_tag="RH033_000944"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDV2990168.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            39595..40062
                     /gene="rlmH"
                     /locus_tag="RH033_000945"
     CDS             39595..40062
                     /gene="rlmH"
                     /locus_tag="RH033_000945"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDV2990169.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            40093..41994
                     /gene="mrdA"
                     /locus_tag="RH033_000946"
     CDS             40093..41994
                     /gene="mrdA"
                     /locus_tag="RH033_000946"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HDV2990170.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            41997..43109
                     /gene="mrdB"
                     /locus_tag="RH033_000947"
                     /gene_synonym="rodA"
     CDS             41997..43109
                     /gene="mrdB"
                     /locus_tag="RH033_000947"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HDV2990171.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            43120..44208
                     /gene="rlpA"
                     /locus_tag="RH033_000948"
     CDS             43120..44208
                     /gene="rlpA"
                     /locus_tag="RH033_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HDV2990172.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            44348..45559
                     /gene="dacA"
                     /locus_tag="RH033_000949"
     CDS             44348..45559
                     /gene="dacA"
                     /locus_tag="RH033_000949"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HDV2990173.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            45669..45932
                     /locus_tag="RH033_000950"
     CDS             45669..45932
                     /locus_tag="RH033_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HDV2990174.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            46033..46674
                     /gene="lipB"
                     /locus_tag="RH033_000951"
     CDS             46033..46674
                     /gene="lipB"
                     /locus_tag="RH033_000951"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HDV2990175.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLSELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            46934..47887
                     /locus_tag="RH033_000952"
     CDS             46934..47887
                     /locus_tag="RH033_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HDV2990176.1"
                     /translation="MDSNNQIETCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHILLL
                     PEGQNFSGLRQRVQEMFPDRQISFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            48096..49061
                     /gene="lipA"
                     /locus_tag="RH033_000953"
     CDS             48096..49061
                     /gene="lipA"
                     /locus_tag="RH033_000953"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HDV2990177.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(49162..49365)
                     /gene="tatE"
                     /locus_tag="RH033_000954"
     CDS             complement(49162..49365)
                     /gene="tatE"
                     /locus_tag="RH033_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HDV2990178.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(49494..50282)
                     /locus_tag="RH033_000955"
     CDS             complement(49494..50282)
                     /locus_tag="RH033_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HDV2990179.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            50378..50761
                     /gene="crcB"
                     /locus_tag="RH033_000956"
     CDS             50378..50761
                     /gene="crcB"
                     /locus_tag="RH033_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HDV2990180.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(50815..51024)
                     /gene="cspE"
                     /locus_tag="RH033_000957"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(50815..51024)
                     /gene="cspE"
                     /locus_tag="RH033_000957"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HDV2990181.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(51199..51759)
                     /gene="pagP"
                     /locus_tag="RH033_000958"
     CDS             complement(51199..51759)
                     /gene="pagP"
                     /locus_tag="RH033_000958"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HDV2990182.1"
                     /translation="MNVSKYVAIFSFVFVQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGQSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT        12857 a        13453 c        13074 g        12571 t
ORIGIN      
        1 ggcgatcttc cgaccgccct ttttcttatt aaatgtgttt acgcgttttt cagaacttcg
       61 ctaacaatct caaccgcttc tttctcaatt tgcttgcgat gttcttcccc gaggaagctt
      121 tcgcaataaa tcttgtacgc gtcttccgtg cctgacggac gcgcggcgaa ccagccgttg
      181 tcagtcatca ctttcagacc gccaatagaa gcaccgttgc ccggagcagc agtcaggcgt
      241 gcggtgatcg ggtcacctgc cagggtgctg gcgctcacca tttccggaga cagtttagac
      301 agcgccgctt tttgtgcgga agtcgcggct gcctgcaaac ggttgtagct cggcgcacca
      361 aagcgttttg ccagttcgtt gtagtgttcc tgcggattct taccggtaac agcggtgatt
      421 tccgccgcca gcagacacat aatgatgccg tctttatcgg tggaccacgg cgtgccgtcg
      481 aaacgcagga aggaagcccc tgcgctctct tcgccgccaa agccgaagct gccgtcgaac
      541 agaccatcaa caaaccattt gaaacctacc gggacttcta ccagcttacg gcccaagtca
      601 ttgaccacac ggtcgatcat cgcagatgaa accagcgttt taccgacagc aacatctttg
      661 ccccactgcg gacggtgctg gaacaggtag ttgatcgcca ccgccaggta gtggttcgga
      721 ttcatcaaac ctgccggagt gacgataccg tgacggtcat aatccgggtc gttagcaaac
      781 gccagatcga acttatcacg cagtgccagc aggcccgcca tcgcacactc ggaggagcag
      841 tccatacgga tcgcaccgtc tttatctagg tgcataaagc ggaaggtttg atcgacctga
      901 tcgttaacga tggtcaggtt gaggttgtaa tactcgccaa tacgcttcca gtattcgata
      961 ccagaaccgc ccagcggatc gacacccagc gtcaggcccg ctttctgaat cgctgccata
     1021 tcaacgatat cggccagccc ttccacgaac ggctgcacca gatcctgctc tttcacatga
     1081 ccggatgcca tcgcttcgtc gagggagata cgcttcacgc ctttcaggcc atcagccatc
     1141 agtgcgttgg ccctgtcttc caccacttta gtgacgttgg tatcagccgg gccaccattt
     1201 ggcggattgt acttgatacc accatcttcc ggcgggttat gagacggtgt aatcacgata
     1261 ccgtctgcca gcgggccacc ttttttattg tgaaccagaa tggcattgga aatggcaggt
     1321 gtcggagtga agccattgtt ttcctgcaca atgatatcaa caccgttcgc tgccagcact
     1381 tccagtacgg aaatgaacgc aggctcggac agggcgtgag tatctttacc cacatagcaa
     1441 gggccagtga tgccgttttt cgcacgttct tcagcaattg cctgagcgat cgccagaatg
     1501 tgcggctcgt taaagctgtg gcgcgctgca ctgccacggt gaccggaagt accgaatttc
     1561 accgcgtgct ccgcattccc tgcttctggt ttcagtacat aatattgcgc cgtcagttgg
     1621 gcgacgttaa tcaaatcact ctgttgtgca ggttggcctg cacgattgtg gattgccatt
     1681 gctttgtcct ttgtctgcaa cgttttagat agttccgcaa accttctcaa tcaattccgc
     1741 cgggaattgc atcgactgca tgatgtgttc gatcatgctg catttacggc cggtgttggt
     1801 gttggtgatc acccaatacg gcgtgccagg cacatgtttc ggcttggtct gattaccatt
     1861 tttcagcagc gtttgttcat ctgccgcaaa gtaaacgcgt gtacgaccgt gcaacgattc
     1921 cgttgcctcg gcaaacgcct gggcgtcaag agaatatagt gtagacaaca gcagcataaa
     1981 gcgattgacc gctcgctttt gctctgcgta ttcatccgaa agcagaagtt cacgcattgc
     2041 gcgaaccttg tctttaatcg ttttgaccgg cttcgcttcg acgatagcag gtgacgcaac
     2101 gcgaacctct ttcgtcaccg gagcagcagg ctgtgatgcg gcggaaaatt tcaacatacg
     2161 ccgtaaaatg tcggatgcgc tctcgccgat atgcttagtg tggctggcaa tatagctgta
     2221 gagttcatca tcaacttcaa tcgttttcat cttaatccag tgcagtgtct tagctgaata
     2281 caagtcgcca ggagtatatg aataaatccc ggcagcggat agcgtcaagc ctagcttgca
     2341 gaaaaactcg gaataaacct tatttgttgc agccctgtgg cacaatggtc aacataatac
     2401 cctaacccga caacgcgaaa aaaagaactt tgccatgaaa ttgaatatcc gcgcgcaaac
     2461 tgcacaaaac cagcacaata attctcccat cgttcttgtc cacggtctgt ttggcagcct
     2521 cgacaacctt ggcgtactgg ctcgcgatct ggtaaacgat cacaatatca tccaggttga
     2581 tatgcgtaac cacggtcttt caccgagaga tccggtaatg aattacccgg cgatggccca
     2641 ggatcttgtt gataccctgg atgcactgca gatcgacaaa gcaacattta tcggtcactc
     2701 catgggcggt aaagcggtaa tggcacttac tgcactggcc cccgatcgga ttgacaagct
     2761 ggtggcaatt gatattgcgc cggtcgatta tcacgttcgc cggcatgatg agattttcgc
     2821 ggccatcaac gcagtcagtg aatcggacgc acaaactcgc cagcaagcag cagcaataat
     2881 gcgccagcat cttaatgaag aaggggtgat tcagtttctg ctgaaatctt ttgttgacgg
     2941 ggagtggcgc tttaacgtgc cggtattgtg ggatcagtat ccgcatattg tgggttggga
     3001 gaaaatcccg gcatgggatc accctgccct gtttatccct ggcggcaatt ctccgtatgt
     3061 tagcgagcag taccgtgatg atttactggc gcaatttcca caggcgcggg cgcatgtgat
     3121 tgctggcgcg ggtcactggg tccatgctga aaaaccggat gcggtattac gcgctatccg
     3181 tcgctatctc aatgattaac gcaactgatt aaaaacccag cgtccgcgtc gctttccgcg
     3241 cgtgggcgtt ggcgtgactc atcgcgctga tgtattatgg cgcgctatcc atccgggcag
     3301 aggcctggct gattgtttcc cccgaagtca ccaagatcat ggccaaagaa caaacggacc
     3361 gtacgacatt agatctgttc gcgcacgagc gtcgaccggg acgaccgaaa actaatccgc
     3421 tttcgcgcga tgaacagctg cgtattaata aacgcaacca gctaaaacgc gacaaagtac
     3481 gtggccttaa gcgtgtcgaa ctgaagctga acgcggaagc tgtcgaggcg ctgaacgagc
     3541 tggcggaatc gcgcaatatg agccgtagcg aactgatcga agagatgtta atgcagcaac
     3601 tggcggcgct gcgtagtcag ggcatcgttt aaatttccac tttcatgtag cacagtgtgc
     3661 agtcctgctc gtttgctgat tccgcaagac tgcctgttct gctatgattg cctttatccg
     3721 tgggcaattt tccaccccca tttcaataag tttcaagagg ttatttcact catggctatc
     3781 actggcatct ttttcggcag cgacaccggt aataccgaaa atatcgcaaa aatgattcaa
     3841 aaacagcttg gtaaagacgt tgccgatgtc catgacattg caaaaagcag caaagaagat
     3901 ctggaagctt atgacattct gctgctgggc atcccaacct ggtattacgg cgaagcgcag
     3961 tgtgactggg atgacttctt cccgactctc gaagagattg atttcaatgg caaactggtt
     4021 gcgctgtttg gttgtggtga ccaggaagat tacgccgaat atttctgcga cgcattgggc
     4081 accatccgcg acattattga accgcgcggt gcaaccatcg ttggtcactg gccaactgcg
     4141 ggctaccatt tcgaagcgtc aaaaggtctg gcagatgacg accactttgt cggtctggct
     4201 atcgacgaag accgtcagcc ggaactgacc gctgaacgtg tagaaaaatg ggttaaacag
     4261 atttctgaag agttgcatct cgacgaaatt ctcaatgcct gatgtgatgc ggcgtagact
     4321 catgtctacg ccgtattaat agataatgcc aatcaaaata attgctacaa atttgtaact
     4381 tttgctgttg tacctgtaca atgtcccggt gttcaagtgg ccttgccgtt gtaaatgtaa
     4441 gctgtgccac gtttttatta acaatatttg ccagggactt gtggttttca tttaggcgtg
     4501 gcaattctat aatgatacgc attatctcaa gagcaaattc tgtcacttct cctaatgaag
     4561 tgaaccgctt agtaacagga cagattccgc atgactgata acaataccgc cctaaagaaa
     4621 gctggcctga aagtaacgct tcctcgttta aaaatcctgg aagttcttca ggagccggac
     4681 aaccatcacg tcagtgcgga agatttatac aaacgtctga tcgatatggg tgaagaaatt
     4741 ggtctggcta cggtatatcg cgtactgaac cagtttgacg atgctggtat cgtcacccgc
     4801 cacaattttg aaggcggtaa atccgtattt gaactgacac agcaacatca ccacgatcac
     4861 ctgatctgcc tcgactgcgg caaggttatc gaatttagtg atgattccat cgaagcgcgt
     4921 cagcgtgaaa tcgccgcaaa acatggcatt cgcctgacta accacagtct ctatctttac
     4981 ggtcactgcg ccgaaggcga ttgccgcgaa gatgagcacg cgcacgaagg caaataagcc
     5041 agcctgaacg agaaaagcca acctgcaggt tggctttttt atacaaggaa aataagaaaa
     5101 gcgagagtta cagctctcac ttatttgtta ttactgcgaa tttcctgcca gaccttatca
     5161 caatcggctt tcaccgcctg atcatttccg gtttgcgttg cctgaataca ctgctgataa
     5221 tccagtacgc ggacactttc ctgctcggca aactgctgat gttgtttctc tttcttcagc
     5281 acgtttaaca cgctctggca ggcttcaatt ttttccggcg aaccttgcgc ggtgttgata
     5341 caggcgctgt aagcctcttt cagacgactg tcttcctgcg gtgcggttga ttgcgcacaa
     5401 gctaccagcc ccgatgccat catggcgatg agaatcagtt ttttcatagc aaattcctca
     5461 aacgtgcggc aggcacaaaa cctgccgcgt cgggcatcag aagatggtga atggtgcgat
     5521 taccatgaat ttcacgtcgc gctcatcctg gaaaatgttg ccgtaaccac cgccccagct
     5581 tgggatgtcg gagtggttgt catattcggt gaaatgcagt ttgaacatcg tgcccttggc
     5641 acgaccgtcc tgaatggtgt aaaccgcatc caggctgtag gcagactctt caatagtccg
     5701 gttcttgtcg tagtacgcat ccggattgct ctgccaggtc gcaggtttag catcccatgc
     5761 gtaaacgtag gaagcgccga tggcgaagcc tggaagattc cagtttttca ggtcatacat
     5821 cgcaccgaag aagaccgctt tttcgccgtt ggcgttgaag tcggaacggt tatcccacca
     5881 gatatccagg cgaccgttcg aggaagcgta ggttggagtc atacgttgca ggaagtatcc
     5941 ttgctgaccg tcagccttaa cccaggtacc ttcgaggcgc aaatccacta cgtcagccgc
     6001 ccggtaacca aaggtcaacg cctgcagcca ggcggtgccg tcataaagat cgttgatgct
     6061 gcgatcgtca actttatcgc gcgtaccata gaactggtag ctggtggtta acgggctacc
     6121 ggcgatatca aatttgtagc tggctttggc aaaatattga tcgatatacc cttcggcctg
     6181 accaaacgcc gcttccagta cgaagttatt tttgaagtcg tacttcgccc caagggagtg
     6241 tagataatca actttggtgg ttttatcgtt ctgataaaac tcatccattt ccagatgcca
     6301 cggtgctttg tattcgttgg tccacatgta ggagaaactc aacgcaccag catcgccgta
     6361 atcaaaattc gccccggcct ccgccccctg ataagtaccc ggcataaagc tccagtgtgg
     6421 cgctaacagc gtctgaccgg ttggctgaat ataacctgcc ctcgcccaaa ccggaccgta
     6481 tttaaatttg gccgctgctt tatacaggct tataccgctt ttatcgccgg accagtcttc
     6541 gtcataggct ttattacttt ttgaaaacgc gatttcgttc gggtgagagc tgtcgccgtt
     6601 ttccgccatt tcaatcgccg taaacacggc aatatcaagg ccgaacatat cagcagcata
     6661 accagactga aaatcgaggt tggcgttcca ggtggagtga gaaaggttgg ttttgtattt
     6721 atcgccgtcg gtaacatctt tacggtcgcg ttcacgctgc cagtaataga taccgccggt
     6781 taaggttgaa tcgtcgatga atccttcggc gcgagcctcc ggaattgcca taaagcccga
     6841 catagctgta acaccggcga tagccagcgc cagcgtacta cgtttgccac taaacgtacg
     6901 catgggttaa tcctctttga cgtataaatt gctgcaccaa aggtgaacag cgaaaaaaat
     6961 aaaaattaaa aaaatagcgt tgagaaaagt ctcgctgact acaggaacag actatttttc
     7021 gcgacgcgaa ttatcaatct ctttctgtaa ccagaatgta agaacatgag caaccgcaca
     7081 tattttgatg atttttgtta caaggcttaa gtttgcggtt gaaaggcttg cagaagaata
     7141 aaagcgtgaa attccagcaa gatgaaagcg gttacatttt atttaattat gttttgtgat
     7201 tttaaattta ttttatggat aaattgcgaa gcggaattaa ttcgcgaaga aaagcagaaa
     7261 aaacgccgca gagcgcggcg tttgggattg tttgcatgtt tcttgccgga tgcggcgtaa
     7321 atgcctcacc cggcctggta gaacttaaaa ttactcgcct actttcgccc aggtatcacg
     7381 cagcccaacg gtgcggttaa ataccggttt ttccgccgta gaatggcggc tgtcgaggca
     7441 gaagtaacct tcacgctcaa actggaatgc tttacccgct accgcgtctt tcagcgacgg
     7501 ttcagcgaaa ccctgtttga tcaccagcga ttccgggtta atcaccgaca ggaaatcatc
     7561 cgcagcacct gggttaggca cgctgaacag acggtcatac aaacggattt caaccggcag
     7621 cgcatgtgcc gcgctcaccc agtgaataac acctttcact ttacgaccat ctgccggatc
     7681 tttgcttaag gtgtcggcgt cataagtaca gaagatggtg gtgatattgc cttcggcatc
     7741 tttctccacg cgttcagcct taatgacgta agcattacgc agacgcactt ctttacccag
     7801 caccagacgt ttgtactgct tgttagcttc ttcgcggaaa tcggcgcgat cgatccaaat
     7861 ctcaccgcta aacggcacct gacggctccc catttccggt ttgttcggat ggttcggcat
     7921 ggtgaccatt tcgccttcgc cctgatagtt ttcgataacc agtttcaccg gatcgataac
     7981 cgccattgcg cgcggagcgt tttcgttgag atcttcacgg atgcaggatt ccagcgacgc
     8041 catttcaatg gtgttgtcct gcttggtcac gccgatgcgt ttgcagaact cacgaataga
     8101 agccgcagtg taaccacgac gacgcagacc ggaaatggtc ggcatacgcg ggtcatccca
     8161 gccttcaacg tgcttgtcgg tcaccagcag gttcagctta cgcttggaca tcacagtgta
     8221 ttccagattc aggcgcgaga attcgtactg gcgcgggtga acaggaatgg tgatgttgtc
     8281 cagcacccag tcgtacaggc gacggttgtc ctggaactca agcgtacaca gagagtgcgt
     8341 aataccttcc agcgcatcgc tgatgcaatg ggtgaagtcg tacatcgggt agatgcacca
     8401 cttgttgcca gtctggtggt gttcagcaaa tttaatgcgg tacagcaccg gatcgcgcat
     8461 tacgataaat ggcgacgcca tgtcgatttt cgcacgcagg caggctttac cttcttcaaa
     8521 actaccggta cgcatttttt cgaacagcgc caggttctct tcaacgctgc ggtcgcggta
     8581 cgggctgttt ttacccggct gcgtcagggt gccgcggtat tcgcggatct gttccggtgt
     8641 cagttcatca acgtacgcca ggcctttatt gatcagttcg atcgcatagg cgtggagctg
     8701 atcaaaataa tcggaggagt aacggacgtt accagaccag tgaaaaccta accactcaac
     8761 gtcgtttttg atcgactcaa catactcgat atcttctttt accgggttag tgtcgtcgaa
     8821 acgcaggttg cactggcctt tatagtcctg ggcgatcccg aagttcaggc agatagattt
     8881 cgcatggcca atatgcagat agccattcgg ctccggcggg aaacgggtgt gtactgtggt
     8941 gtgcttacca ctggccagat cttcatcgat gatctgacga ataaagttag tcgggcgggc
     9001 ttctgcctca ctcatcgtgg attcctcaaa gcgtaaacaa cgtataacgg cgtatgatct
     9061 tataagcggg acaggctgac aactgttagt tagtgaaaat cgaaaatctc actgagatta
     9121 tggggatgat tgatgaaaaa aaaagcggca gagaagatct ccaccgcttg tcattgttgg
     9181 atgcgacgct caagcgtcgc atcaggcata aagcagatta ctttttgatt tcatacagcg
     9241 gtgtttgacc cgccacaaca tggccctgag ctttaatgat caggccgctg aaatcgtcga
     9301 tattgctgca aaccaccggg ctaatcatcg agcgggcgtt agcgttcagg taatccagat
     9361 ccatttccag aatcggttgt cctgcgctta cctgcgcgcc ctcttccacc agacgtttaa
     9421 agcctttacc ttccagcgct acggtgtcga tacccatatg gacgacgatc tccgcgcctt
     9481 tttcggtttc cagacagaac gcgtggttgg tgttgaagat tttcacgatt gtccctgcgg
     9541 ctggtgatac gacgatttta tctgtcggtt tcaccgccac accgtcaccc accgctttgc
     9601 tggcgaatgc ttcgtcagga acctgatcca gtgccacaac atcaccggta atcggcgata
     9661 ccagctccgc gatagacacc gcgtttggta cagcctgcgg ttttgctaca ggcgcggcag
     9721 ttgcaggcgc agtttcagct gacgcagcgg ctaccggacc acgggcaacg actttcttca
     9781 tcgcatcgcc gatggattct gctttcgcgc ccacaatcac ctgaatagtc tgtttgttca
     9841 gttttactac cccagaagcc cccagacgtt tacacatcac atcgttgacg cgagctgagt
     9901 caaccacggt aaggcgcaga cgagtaatac aggcgtcaat ggctttcagg ttgtcagtac
     9961 cgccaaccgc agcaatatag ttggttgcca gttgattgag accttcttcc gtgttgctgt
    10021 tggcttcttc agtaacgatc tcgtcttctt tatcttcacg gcccggcgtt ttcaggttga
    10081 acatgcggat aaccaaactg aacaccacga agtagatagc gaagaagaca acacccatca
    10141 ccagcagcat ccagacgttc tggctggcgg ccggcaggtt atacatcaac gcgtagtcga
    10201 tagcccccgc agagaaggag aagcccgcgt ggatacccag cagcgttgcc acaaacaggc
    10261 tgataccggt cagcagtgcg tgcaggaggt acagcagcgg cgcaaggaac atgaacagga
    10321 attccagcgg ctcagtcaca ccggtcagga acgcagtaac agcaacagaa agcagcatac
    10381 cgccaaccat cggacgacgc tctttcggtg ctgcaaagta catcgccagc gccgcacccg
    10441 gcagaccgaa catcatgatc gggaagaagc cggacatgaa catccccgcg gtgccgtcac
    10501 cggcatagaa gcggttaatg tcgccgtgga aaaccgtacc cgccgcgttg gtgaattcac
    10561 caatctggaa ccaggcgatg gtgttcagta cctgatgcag accggttggg atcagcagac
    10621 ggttgatgaa accaaagata ccggaaccca gcgcgcccgc agaaacgatc cactcgccgc
    10681 ctgcatggat agcgtgctgt accggcggcc agacgtaacc aaaaatggcc gccagcacca
    10741 ggcagaagaa gctggtggca atcggcacaa agcgtttgcc gccgaagaag ctcaggaagt
    10801 ccggcagttt aatatcggac caacggttat aggctgcacc accgaccaga ccggtaatga
    10861 tacccgccag tacacccatg ttaatttccg ggttgatggt caccatcgct ttggttaaca
    10921 caaagtaacc taccgcaccc gccagtgccg ccgcacctgc gctgtctttc gaccagctgg
    10981 atgccacgcc gatggcgaag attaatgcga ggttatcaaa aatcgcaccg cccgcctggg
    11041 caataaacgc aacgttaagt aaatctggct gaccgaatcg cagcaacagc gccgccaccg
    11101 gcagcaccgc gatagggagc tgtaacgccc taccgagtcg ctggaaaaaa cctaaaatat
    11161 tcatcttatt ccccctacga gaaccctatt tggctcgttt aaagccgtat ttttattttg
    11221 ctgcaaattg tactgccgat gttctgtaat cagattgtta gatcatctgc tacagagtgt
    11281 gtgaaaattt aattcgtatc gcaaattaaa cgcgtgtctt ttgtgagttt tgtcaccaaa
    11341 tatcgttatc atcactccct tttactggct aaaccagaaa acttatttta tcattcaaaa
    11401 aatcaggtcg gattgacgcc tgtctgcgca aatccaggtt acgcttaaag atgcctaatc
    11461 cgccaacggc ttacatttta cttattgagg tgaataatga gactgatccc cctgactacc
    11521 gctgaacagg tcggcaaatg ggctgctcgc catatcgtca atcgtatcaa tgcgttcaaa
    11581 ccgactgctg atcgcccgtt tgttctgggc ctgccgactg gcggcacgcc gatgaccacc
    11641 tataaagcgt tagtcgaaat gcataaagca ggccaggtca gctttaagca cgttgtcacc
    11701 ttcaacatgg acgaatatgt cggtctgccg aaagagcatc cggaaagcta ctacagcttt
    11761 atgcaccgta atttcttcga tcacgttgat attccagcag aaaacatcaa ccttctcaac
    11821 ggcaacgccc cggatatcga cgccgagtgc cgccagtatg aagaaaaaat ccgttcttac
    11881 ggaaaaattc atctgtttat gggcggtgta ggtaacgacg gccatattgc atttaacgaa
    11941 ccggcgtctt ctctggcttc tcgtactcgt atcaaaaccc tgactcatga cactcgcgtc
    12001 gcgaactctc gtttctttga taacgatgtc aatcaggtgc caaaatatgc cctgactgtc
    12061 ggtgttggta cattgctgga tgccgaagaa gtgatgattc tggtgctggg tagccagaaa
    12121 gcactggcac tgcaggccgc cgttgaaggt tgcgtgaacc acatgtggac catcagctgt
    12181 ctgcaactgc atccaaaagc gatcatggtg tgcgatgaac cttccaccat ggagctgaaa
    12241 gttaagactt taagatattt caatgaatta gaagcagaaa atatcaaagg tctgtaattg
    12301 ttatccctgc cctggctcct tgctcagggc aatatttttt aaaatcgggg gtcagaatgt
    12361 atgcattaac ccagggccgg atctttaccg gccacgaatt tcttgatgac cacgcggttg
    12421 ttatcgctga tggcctgatt aaaagcgtct gtccggtagc ggaactgccg ccagagatcg
    12481 aacaacgttc actgaacggg gccattctct cccccggttt tatcgatgtg cagttaaacg
    12541 gctgcggcgg cgtgcagttt aacgatactg ctgaagcagt cagcgtagaa acgctggaaa
    12601 tcatgcagaa agccaatgag aaatcaggct gtactaacta tttgccgacg cttatcacca
    12661 ccagcgacga gctgatgaaa cagggcgtgc gcgttatgcg cgagtacctg gcaaaacatc
    12721 cgaatcaggc gttaggtctg catctggaag gtccgtggct gaatctggta aaaaaaggca
    12781 cccataatcc gaattttgtg cgtaagcctg atgccgcgct ggtcgatttc ctgtgcgaaa
    12841 atgccgatgt cattaccaaa gtgaccctgg caccggaaat ggttcctgcg gaagtgatca
    12901 gtaaactggc aaatgccggg attgtggttt ctgctggtca ctccaacgcg acgttgaaag
    12961 aagcaaaagc cggtttccgc gcggggatta cctttgccac ccatctgtac aacgcgatgc
    13021 cgtatattac cggtcgtgaa ccgggcctgg cgggcgcgat cctcgacgaa gctgacattt
    13081 attgcggtat tatcgctgat ggcctgcatg ttgattacgc caacattcgc aacgctaaac
    13141 gtctgaaagg cgacaaactg tgtctggtta ctgacgccac cgcgccagca ggtgccaaca
    13201 ttgaacagtt catttttgcg ggtaaaacaa tatactaccg taatggactt tgtgtggatg
    13261 agaacggtac gttaagcggt tcatccttaa ccatgattga aggcgtgcgt aatctggtcg
    13321 aacattgcgg tatcgcactg gatgaagtgc tgcgtatggc gacgctctat ccggcgcgtg
    13381 cgattggcgt tgagaaacgt ctcggtacac tcgccgcagg taaagtagcc aacctgactg
    13441 cattcacacc tgattttaaa atcaccagga ccatcgttaa cggtaacgag gtcgtaactc
    13501 aataagagaa agtatgacac caggcggaca agctcagata ggtaatgttg atctcgtaaa
    13561 acagcttaac agcgcggcgg tttatcgcct gattgaccag tacgggccaa tctcgcggat
    13621 tcagattgcc gagcaaagcc agcttgcccc cgccagcgta accaaaatta cgcgtcagct
    13681 tatcgaacgc gggttgatca aagaagttga tcagcaggcc tccaccgggg gccgccgcgc
    13741 tatctctatt gtcaccgaaa cccgcaattt ccacgcaatc ggcgtacggc ttggtcgtca
    13801 cgacgccacc atcactttgt ttgatctcag tagcaaagtg ctggcagaag aacattaccc
    13861 gctgccggaa cgtacccagc agacgctgga acatgccctg ctgaatgcca ttgctcagtt
    13921 tattgatagc taccagcgca agctgcgcga gctgatcgcc atttccgtga tcctgccagg
    13981 gcttgttgac ccggacagcg gcaaaattca ttacatgccg catattcagg tagaaaactg
    14041 ggggctggta gaagcactgg aagaacgttt taaagtgacc tgtttcgtcg gtcacgatat
    14101 ccgtagtctg gcgctagcgg agcactactt cggtgcaagt caggattgcg aagactccat
    14161 tctggtgcgt gtacatcgcg gaaccggggc cgggattatc tctaacgggc gcatttttat
    14221 tggccgcaac ggtaacgtcg gtgaaattgg ccatattcag gtcgaaccgc tgggtgaacg
    14281 ctgccactgt ggcaactttg gctgcctgga aactatcgct gccaacgctg ccattgaaca
    14341 acgggtgttg aatctgttaa agcagggcta ccagagccgc gtgccgctgg acgactgcac
    14401 cataaaaact atctgcaaag ctgcgaacaa aggcgatagc ctggcgtcgg aagtgattga
    14461 gtatgtcggt cgtcatctgg gcaaaaccat cgccattgcc atcaacctgt ttaacccgca
    14521 aaaaattgtt attgccggag aaatcaccga agccgataaa gtgctgctcc ctgctattga
    14581 aagctgcatt aatacccagg cgctgaaggc gtttcgcact aatctgccgg tggtacgttc
    14641 tgagctggat caccgctcgg caatcggcgc ttttgcgctg gtaaaacgcg ccatgctcaa
    14701 cggtattttg ctccagcatt tgctggaaaa ttaatgtgct tttatagtgg cgcttattgt
    14761 tgtcaatatt ctgggtagtc catgaccatt aaaaatgtaa tttgcgatat cgacggcgtg
    14821 ctgatgcacg ataacgtcgc cgtaccgggt gcagcggaat ttttgcacgg gattatggat
    14881 aaaggcctgc cgctggtgtt gctgaccaac tatccttcgc agaccgggca agatctggcg
    14941 aatcgctttg ccactgccgg tgtcgatgta ccggatagcg tgttttatac ctccgcgatg
    15001 gcgaccgccg atttcctgcg tcgccaggaa ggcaagaaag catatgtggt gggcgaaggc
    15061 gcactgattc atgaactgta caaagccggt ttcactatta ccgatgtgaa ccctgatttt
    15121 gtgattgttg gcgaaacgcg ttcctacaac tgggacatga tgcataaagc agcctatttc
    15181 gtcgctaacg gtgcacgctt tatcgccacc aacccggaca cccacgggcg cggtttttat
    15241 ccggcctgtg gcgcgttgtg tgcagggatc gagaaaatct ccgggcgcaa accgttctat
    15301 gttggaaagc cgagtccgtg gatcatccgc gcggcattaa acaaaatgca ggcgcattcg
    15361 gaagaaacgg tgattgtcgg cgataacttg cgtaccgata ttctggcggg cttccaggcc
    15421 ggtctggaga cgattctggt gctttctggt gtttcgtcac tcgacgatat cgacagtatg
    15481 ccgttccgcc caagctggat ttacccgtcg gtcgctgaaa tcgacgttat ctgaatagca
    15541 accacgtctg cggacgtggt tggctctgat gacaagcgcc aaactgcctg atgcgctacg
    15601 cttatcaggc ctacgcagct cctgcaatat attgaatttt catgcttttg taggccggat
    15661 aaggcgttca cgccgcatcc ggcatgaaca aatcgcactt tgtcagcaat ttggctcccg
    15721 taaaatacaa ccattcaata aaacccgcta aaaattatcg attcatcaat aattacatcc
    15781 tcacaccaga ccttttttca tcaatcgaca atcaccatta cgttttttat tttttcaccg
    15841 ccaaatcgct tgcgcacagt gctgctttgc ggcatttttt taaacaagca aacacaacaa
    15901 gcaacaaata ccaggttaac ggagaaggtt atgtgttcaa tttttggcgt attcgatatc
    15961 aaaacagacg cagttgagct gcgcaagaaa gcactcgagc tgtcacgcct gatgcgtcat
    16021 cgtggcccgg actggtccgg tatttatgcc agcgataacg ccattctcgc ccacgaacgt
    16081 ctgtccattg ttgacgttaa cgcgggggcg caacctctct acaaccaaca aaaaacccac
    16141 gtgctggcag taaacggtga aatctacaac caccaggcat tgcgcgctga atatggcgat
    16201 cgttaccagt tccagaccgg gtctgactgt gaagtgatcc tcgcgctgta tcaggaaaaa
    16261 gggccggaat ttctcgacga cttgcagggc atgtttgcct ttgccctgta cgacagcgaa
    16321 aaagatgcct acctgattgg tcgcgaccat ctggggatca tcccactgta tatgggctat
    16381 gacgaacatg gtcagctgta tgtggcctca gaaatgaaag ccctggtgcc ggtatgccgc
    16441 acgattaaag agttcccggc ggggagctat ttgtggagtc aggacggcga gatccgttct
    16501 tattatcacc gcgactggtt cgactacgat gcggtgaaag ataacgtgac cgacaaaaac
    16561 gagctgcgtc aggcactgga agaatccgtt aaaagccatc tgatgtctga tgtgccttac
    16621 ggtgtgctgc tatctggtgg cctggattcc tcaattattt ccgctatcac caagaaatac
    16681 gcagcccgtc gcgtggaaga tcaggaacgc tctgaagcct ggtggccgca gctgcactcc
    16741 tttgctgtag gtctgccggg ttcaccggat ctgaaggcag cccaggaagt ggcaaaccat
    16801 ctgggcactg tgcatcatga aattcacttc actgtacagg aaggtctgga cgccatccgc
    16861 gacgtgattt accacatcga aacctatgac gtgacgacaa tccgcgcttc aacaccgatg
    16921 tatttaatgt cgcgtaagat caaggcgatg ggcattaaaa tggtgctgtc cggtgaagga
    16981 tctgatgaag tgtttggcgg ttatctatac ttccacaaag cgccgaatgc caaagaactg
    17041 catgaagaga cggtgcgtaa actgctggcc ctgcatatgt atgactgcgc gcgtgccaac
    17101 aaagcgatgt cagcctgggg cgtggaagca cgcgttccgt tcctcgacaa aaaattcctc
    17161 gacgtggcga tgcgcattaa cccacaggat aaaatgtgcg gtaacggcaa aatggaaaaa
    17221 cacatcctgc gtgaatgttt tgaagcgtat ctgcccgcaa gcgtggcctg gcggcagaaa
    17281 gagcagttct ccgatggcgt cggttacagt tggatcgaca ccctgaaaga agtggcggcg
    17341 cagcaggttt ctgatcaaca gctggaaact gcccgcttcc gcttcccgta caacacgccg
    17401 acgtccaaag aagcgtatct gtaccgcgag atctttgaag aactgttccc gcttccgagc
    17461 gccgctgagt gcgtgccggg cggtccttcc gtcgcgtgtt cttccgctaa agcgatcgaa
    17521 tgggatgaag cgttcaaaaa aatggacgat ccatctggtc gtgcggttgg tgttcaccag
    17581 tcggcgtata agtaagaaat gcgaaacagc cccggtaaaa tccggggctg tttcagttat
    17641 tgaggtgctt gttgattctg atatgcagta aggaaatgtt ccgggtttgc cacatcactg
    17701 tttgacaact catcactgta ggccaccgac caggtcgcct gctcattcgg aatatgcagg
    17761 ctgccattcc cctctttcag gcgtaataac tcaaaagatt tgcccttaat tacggcggta
    17821 taggattttt caccttcctg taatgctttc actttacagc gaaaaatctg gccatcgatg
    17881 tcattatcaa catgcgatac ctgaatgagg gcttcattag aggctttcgg ccctatccgc
    17941 atcatccaca ccacagcgcc ttcctgccca cgatagccaa aaacatattt accgatttcc
    18001 ggtttgaccg tatcggcaaa cgcagattgc ataagtaata acgcacccat cagcgccgtc
    18061 ataaaacgca gcatagtatt ccctcaataa atcgcctgaa gtggtagcag agatgcaggc
    18121 gtagcgatcc ctgccctgtc ctgttgcaac gggccagtat ttcatagatt aatttcccac
    18181 cactattgta gaattccgaa tttcatcctg tatgacaaaa gaatggctgt agggtgggga
    18241 aaaaacacaa agtaaacaat aattgacgaa tatagcgcca tgctgttcgc aacctaacca
    18301 aacagtcact ttcgagcaat tttccttgaa aaagaggttg acgctgcaag gctctatacg
    18361 cataatgcgc cccgcaacgc cgataaggta tcgcgaaaaa aagatggcta cgtagctcag
    18421 ttggttagag cacatcactc ataatgatgg ggtcacaggt tcgaatcccg tcgtagccac
    18481 catctttttt tgcgggagtg gcgaaattgg tagacgcacc agatttaggt tctggcgccg
    18541 caaggtgtgc gagttcaagt ctcgcctccc gcaccattca ccagaaagcg ttgtacggat
    18601 ggggtatcgc caagcggtaa ggcaccggtt tttgataccg gcattccctg gttcaaatcc
    18661 aggtacccca gccatcttct tcgagtaagc ggttcaccgc ccggttattg gggtatcgcc
    18721 aagcggtaag gcaccggttt ttgataccgg cattccctgg ttcgaatcca ggtaccccag
    18781 ccatcgaaga aacaatctgg ctacgtagct cagttggtta gagcacatca ctcataatga
    18841 tggggtcaca ggttcgaatc ccgtcgtagc caccaaattc tgaatgtgtc gaatatgttc
    18901 ggcaaattca aaaaccaatt tgttggggta tcgccaagcg gtaaggcacc ggattctgat
    18961 tccggcattc cgaggttcga atcctcgtac cccagccaca ttaaaaaagc tcgcttcggc
    19021 gagctttttg cttttctgcg tatattcaat gccgaatgtg atgctgacac gtcttatcct
    19081 tcaaagtcgg atgcgacgct ggcgcgtctt atccgaccta cggttggcat gcttccggca
    19141 atgttgtaag gctacaaccc taacgcatac ttcagcgcct gacgtttcaa cacgccagca
    19201 cgctccgccg ccatcaaacc aagattacgc acaaaacgca gcggcggcag attattactg
    19261 aatccggcat aaaacagatc cataccactt tgcataatga agttatccgc catgcgccgc
    19321 atctggtaac gcttgaggat cggataactg gcccacgctt cgccgtagct gcgggcgttg
    19381 accagaacat caattagggc atcgacatca cgataaccaa gattcacccc ctgccccgcc
    19441 agcggatgga tggtatgcgc ggcatcgccc accagcgcaa gccccggctg cacatactgc
    19501 aacgcatgtc ggcgcgtcag cggaaacgca ccagcggcaa gcggtgtcac gtaacccaga
    19561 cacgacggga aatgcttcgc gatttccgtc tggagctgcg ccatattcat attctgcaac
    19621 tggcgaatac gcgccggaga gtcataccac accagcgatg cccagttatc aaacaacggc
    19681 agaaacgcac gcggtccgtc cggagtaaat tgctgccagg tgctatcgcc gggatcgttc
    19741 tcgcactgga cgctaatcaa catgcacgac tgcgcatact gccaggcatg aacgccaatt
    19801 cccgctatct gccgcacctg cgaatttgcg ccatcggcac caatcaccag cttcgcgcgg
    19861 atcgtttcac cgcccttcag ttccagctcc tgaagatcat tatggcgatg cagcgcaatc
    19921 agcgagcctg gcacacgtaa cgttactttc ggatgcgctt ccagcgcctg ccacagcgcc
    19981 tgttgcagga cagtgttttc caccatgtag ccaagcaacg gtagcttaag ttcagcggcg
    20041 tcaaacacca catgcgccgt ttcccactcc cacgtttcca gtctgcggta aggatggcaa
    20101 cgcatagcct gtactgcatc ccagacccct aaccctttaa gcaatgatac cgaagccgca
    20161 ctaatcgccg agatccgcac gtccggctgg ctgtcagcga caaacggtgc aggttgggca
    20221 tgttcgataa ccattaccga aaatccgtgc tgagccagtc ccagcgccag tgcgccgccg
    20281 accattcctc cgccgacaat ggcaatttcc gttggttgat ttgtcatggt caatcatcct
    20341 gttagcaata tgctttaagt ttacaggatt tttaccccca agggctgata acgctcatac
    20401 tggtcacaac ggcagcaaag cattacacta tgcgccctgc attcctggct actatttcgc
    20461 aagagcaagt cgatgaccaa aaaactccat attaaaacct ggggctgtca gatgaacgag
    20521 tacgattcat cgaagatggc cgatctgctg gatgccaccc acggctatca actgaccgat
    20581 gtggcggaag aagcggatgt gctgctgctg aacacctgct caatccgcga gaaggctcag
    20641 gaaaaagtct tccatcagtt gggtcgctgg aaactgttaa aagagaagaa tccagacctg
    20701 attatcggcg tcggcggctg cgtggcatcg caagaaggcg agcacattcg ccagcgcgcc
    20761 cactatgtcg atattatttt tgggccgcaa acgctgcacc gtctgccgga gatgatcaac
    20821 tccgtgcgcg gcgaccgcag cccggttgta gatatcagct tcccggaaat cgagaagttt
    20881 gaccgtctgc cggaaccgcg cgccgaaggg ccgaccgcgt ttgtctccat catggaaggc
    20941 tgcaataaat attgcaccta ctgcgtagtg ccttacaccc gtggtgaaga ggtaagccgt
    21001 ccgtccgacg atattctgtt tgaaattgcc cagcttgcgg ctcagggcgt gcgtgaagtc
    21061 aacctgctcg gtcagaacgt gaacgcctgg cgtggtgaga actacgacgg caccaccgga
    21121 tcgtttgccg acctgctgcg tctggttgcg gcgatcgacg ggatcgatcg tattcgcttt
    21181 accaccagcc atccgatcga attcaccgac gatatcatcg aagtgtaccg cgacacgccg
    21241 gagctggtga gtttcctgca tctgccggta cagagcggtt ccgatcgcat tctgaacctg
    21301 atgggacgta cccatacagc gctggagtac aaagcgatca tccgtaaact gcgtgcggcg
    21361 cgtccggata ttcagatcag ttctgacttc atcgttggct tccctggcga aaccaccgaa
    21421 gacttcgaga aaacgatgaa gctgattgcc gacgtcaatt tcgacatgag ctacagcttt
    21481 atcttctccg ctcgtccagg tacaccagcc gccgatatgg ttgatgatgt tccggaagaa
    21541 gagaagaagc agcgcctgta tattctacaa gaacgtatca atcagcaagc aatggcatgg
    21601 agccgccgga tgctcggtac cacccagcgt attctggtag aaggtacatc gcgtaagagc
    21661 atcatggagc tttccggccg tactgaaaat aaccgcgtcg tcaacttcga aggtacgccg
    21721 gatatgatcg gtaaattcgt cgatgtagaa attaccgacg tctacccgaa ctctctgcgc
    21781 ggtaaagtgg tgcgaactga agatgaaatg ggtctgcgcg tggcagaaac accggaatcg
    21841 gtgattgccc gtacccgcaa agaaaacgac cttggcgtgg gttattatca gccgtaatac
    21901 tcaggcccgc cgttccggtg ggccttgttt tttctctctt tatccccatc tttatcgcaa
    21961 tgcttgcgcc tttttcctgt ggcgtgaata atttccttat aggccggtga atgttcagct
    22021 tccggcggca tacagataag aggaacggtt tgaacataga cactcgcgaa atcaccctgg
    22081 agccagcaga caacgcgcgt ctgttgagcc tgtgcggccc gtttgatgac aacatcaaac
    22141 aactcgaacg ccgtctcggc atcgagatca atcgccgcga taaccacttt aaactgaccg
    22201 gccgtccgat ttgcgtcacc gctgcggcag acattctgcg tagcctgtat gtcgataccg
    22261 ccccgatgcg cggtcagatt caggatatcg aaccggaaca gatccacctt gcgattaaag
    22321 aagcgcgggt actggagcaa agcgcggaga gcgtgccgga gtacggcaaa gcggtcaata
    22381 tcaaaaccaa acgcggcgta attaagccgc gcacgccaaa ccaggcgcag tacatcgcca
    22441 atattctcga ccatgacatt accttcggcg ttggcccggc gggtacgggt aaaacctacc
    22501 tggcagtggc tgcggcagtt gatgccctgg agcgtcagga aattcgccgt attctgctga
    22561 ctcgtccggc agtagaagcc ggtgagaaac tgggcttcct gcctggcgat ttaagccaga
    22621 aagtagaccc gtatctgcgc ccgctgtacg acgcgctgtt tgaaatgctg ggctttgaga
    22681 aagtcgagaa actgattgag cgcaacgtta ttgaagtcgc accgctggcc tatatgcgtg
    22741 gtcgtacgct gaacgacgcg tttatcattc ttgatgagag ccagaacacc accatcgaac
    22801 agatgaagat gttcctgacc cgtatcggtt ttaactcaaa agcggttatc accggcgacg
    22861 tcacacagat cgacctgccg cgtaatacca agtctggttt gcgtcatgcc atcgaagtac
    22921 tggctgatgt cgaagagatc agctttaact tcttccacag cgaagacgtg gttcgtcacc
    22981 cggtggtggc gcgtatcgtt aacgcctatg aagcctggga agaagccgaa caaaaacgta
    23041 aagcggcgct ggcagcagaa cgcaagcgcg aagaacagga acaaaaatga gtcaggtgat
    23101 cctcgattta caactggcat gtgaagataa ttccgggtta ccggaagaga gccagtttca
    23161 gacatggctg aatgcggtga tcccgcagtt tcaggaagaa tcggaagtga ctattcgcgt
    23221 ggtcgatacc gccgaaagcc acagtctgaa tctgacctat cgcggtaagg ataagccgac
    23281 caacgtgctc tccttcccgt ttgaagtacc gcctggcatg gaaatgtcgc tactgggcga
    23341 tctggttatc tgccgtcagg tggttgagaa ggaagctcag gagcaaggca aaccactgga
    23401 ggcgcactgg gcgcatatgg tggtgcacgg cagtctgcat ttgttaggtt acgatcacat
    23461 cgaagatgac gaagcagaag aaatggaagc catcgaaaca gagattatgc ttgctctggg
    23521 ctatgaggat ccgtacattg ccgagaaaga ataatttgcc aggcatttga ctggcagacg
    23581 tccccgccgt tggtgattaa cgacggcgtt ttgattaact aacatgagaa cccatacgac
    23641 gccatgagcg acgacaattc acacagtagt gacacgataa gcaacaagaa gggatttttc
    23701 tccctgttac tcagccaact tttccacggt gaaccgaaaa accgtgacga actgctggcg
    23761 ctgatccgtg attccgggca gaacgacctt atcgacgaag atacgcgcga tatgctcgaa
    23821 ggggtgatgg acatcgcaga ccaacgcgtt cgcgacatca tgatcccccg ctcccagatg
    23881 attaccctga aacgcaacca gacgctggac gaatgccttg atgtcatcat cgagtccgcc
    23941 cactcacgtt tcccggtgat tagcgaagac aaagatcaca ttgaagggat tctgatggcg
    24001 aaagacttgc tgccgtttat gcgcagcgat gctgaagcct tcagcatgga caaagtgtta
    24061 cgtcaggcgg ttgtcgttcc tgaaagtaag cgcgtagacc ggatgctgaa agagtttcgc
    24121 tctcagcgtt accacatggc gatcgtcatt gacgaattcg gtggggtttc cggtctggtg
    24181 accattgaag acatcctgga actgattgtt ggtgagattg aagacgagta tgacgaagaa
    24241 gatgatatcg acttccgtca gctgagtcgt catacctgga ccgtgcgcgc actggcttct
    24301 attgaagact tcaacgaagc gttcggcacc cactttagcg atgaagaggt tgacactatc
    24361 ggtggtctgg tgatgcaggc atttgggcat cttccggcgc gtggcgaaac catcgacatc
    24421 gacggttacc agttcaaagt ggcgatggcc gacagtcggc gtattattca ggttcatgtc
    24481 aaaatcccgg atgactcacc ccagccgaag ctggatgaat aaaaccgaaa ctggatagat
    24541 aactacatgg cttttgcctc attaattgaa cgccagcgca ttcgcctgct gctggcgtta
    24601 ttattcggtg cctgcggaac gctggccttc tctccttacg acgtctggcc tgcggcgatt
    24661 atttcgctga tggggcttca ggcgttgacc tttaaccgcc gtccactcca gtctgccgct
    24721 attggctttt gctggggatt tggcctcttt ggcagcggta ttaactgggt ctatgtcagc
    24781 atcgcgacct ttggcggaat gcctggcccg gttaacatct tcctggtggt actgctggcg
    24841 gcgtatttgt cgctgtatac cggactgttt gctggcgtgc tgtcgcgtct gtggccgaaa
    24901 accacctggc tgcgcgtggc gattgccgcc cctgctctct ggcaagtgac cgagtttctg
    24961 cgcggttggg tactaacagg cttcccgtgg ttacagttcg gctatagcca gattgatggc
    25021 ccgttaaaag ggctggcacc gataatgggc gtggaagcca ttaacttcct gctgatgatg
    25081 gttagcggcc tgctggcact ggcgttggtc aaacgcaact ggcgtccgct ggtggtggcc
    25141 gtcgtgctgt ttgcccttcc cttcccgctg cgttacatcc agtggtttac cccgcaaccg
    25201 gaaaaaacta ttcaggtttc gatggttcag ggcgatattc cgcaatcgct gaaatgggac
    25261 ggagaccagc tacttaatac gctgaagata tactacaacg caacggcacc gctgatgggc
    25321 aaatcatcgt tgattatctg gccggagtcg gcgataaccg atctggaaat taatcagcaa
    25381 ccgttcctca aagctctgga cggtgagttg cgcgataaag ggagctcgct ggtcaccggg
    25441 attgtcgacg cgcgtctcaa taaacagaac cgctacgata cctacaacac catcatcaca
    25501 ctgggtaaag gcgcaccgta cagctatgaa tcagccgatc gctataacaa aaaccatctg
    25561 gtgccgtttg gcgagtttgt cccactggag tcgatactgc gtccgttagc accgttcttt
    25621 gatttgccga tgtcttcatt cagccgcgga ccatatattc agccaccgct gtcgttgaat
    25681 ggcattcaac ttactgcggc catttgctac gaaatcattc tcggcgagca agtgcgcgat
    25741 aacttccgcc cggataccga ctatctgctg actatctcca acgatgcgtg gtttggtaag
    25801 tctattggtc catggcagca cttccagatg gcgcgaatgc gtgcgctgga gctggcgcgc
    25861 ccactgttgc gcagcaccaa caacggcatt acggcggtga ttggcccgca gggtgagatt
    25921 caggcgatga tcccgcagtt cacccgcgag gtgttaacca ctaacgtgac gccgactacc
    25981 ggactcacac cgtacgcacg taccggcaac tggccgctgt gggtgctgac ggcactgttt
    26041 ggttttgcag ctgtgctgat gagtctgcga gcacgtaaac attaatgtgc ctgatgtatc
    26101 cggcatgaac aaaacatact ttctcattga gttgtggggc atgattatca tgcccttttt
    26161 tcttacggtt cagggataca agccacatcg ccatattggg gatgccgaat aattgcctgc
    26221 ggatcaagcg agcgcgtcct gtccatttgg attaacctgt tcttcgccat aacaaatatc
    26281 tttctaccat ccgctgaaaa acgaaggtga ttaacgtagc tggcaggata aacgccaata
    26341 atctgcccgg cattaatatt gagcaggatg acataacctt gcaggccata tgacaccaac
    26401 gctaaatatt gaccgtccgg gctaataccc tcaacggaat aaacgctcct gctacaccat
    26461 tgcgtcataa tgctggcttg ttgatcccct acgcttatac gtgaaatccc ctggctatcg
    26521 gcaaaaataa tctcgccatt ttttggtcgg aatcccatcg cagaaacgat accacgcgca
    26581 tcacctgtgg gaaagtcttc tttctgatgc gtgatggtat tgatgcgtac aatgtcgcct
    26641 ctgaccatac cgggtatcaa caatgttttt tcatccgggg aaagtgccat aacccaggcg
    26701 cctggtttac cgctcgtccc cgtcccgtcg tatttctcca gcgggatatc cgtcacatgc
    26761 tgataagtca ccagatcaat ggctgacaca ctattactgg tgcgattcat cacccaggct
    26821 tttgtgccat cacgcgagag gataaggctg tcaggatggg cgccggtggt aatcgactga
    26881 ataatctccc ccgtcgccac gttaatgacc gatactgcat tcgcttcaaa tgaagtcgca
    26941 taaatcaact tttcatccag gcttaatgcc agcgatctga cgccctgatc tttacagcca
    27001 ccacaatgtt tgactgactt ttctttcgct atgggcgctg taaaggtatg caggattttc
    27061 ttttgtccga ggtcgaaaac aacaatgctg ttggtgtttt caattgcggc atacaacgta
    27121 ttaccatccc gactgatcgc catatcatgg atatcggcaa tctcagcgag taaatcacca
    27181 ctggcctgct cacgacttac cggagcggat aatgtttccc tgagggcgtt gcgctcctcc
    27241 actaactgct gctttttttg tcgtcgctct tgttcaatct cctgcggata aaacgcttca
    27301 cgcaggcttt gcctgacctg taaaactgat tcatcaatct gctgctgcac aaaccagata
    27361 gtcccggtca gaagaagagt gaccgcaaca ataatcccgc cttccgtaac gagccgattt
    27421 tgcccaacta aacgcgcact cagccaaccc aacgcgcccc ccgcaatcag gccgccaatg
    27481 tggcaagcgt tatctattcc tgcttgccgg gcaccataca acagtgtcag cgccaccata
    27541 cccaacagag aaaatactcg ccgctggttt ttatggtgtg tacccgcacc tgaagaaagc
    27601 tgtgttgcta tcgcagcgcc cgctattccc ataattgccc cggatgcccc aacgctgatg
    27661 actatctgct cactttcgtg taacgcataa caggcgctga tcagcccacc aaagatgccg
    27721 gagaataacc agataatcag gagtcgccat ttccccagaa tacgttccac cgctcccccc
    27781 accaccagga gtgcaaacat attcatcagc aaatgagaaa cactgctgtg cagaaagaca
    27841 ctgctcaata agcgccagga ttcacccgac aacgtgagag aagcaacgtt agcaccgaga
    27901 tggataagtg cggattgatc gacttcttca taactcatcc attgtgcgag gtaacaaaat
    27961 atcccaacac agagcgcaaa aaggataagg gttattgcgg gtaattgaac atttaacggc
    28021 ttaaccgaag atgcagacat aaggtgattc ttccttgaaa agacaggtaa aaagcatttt
    28081 tcccgtgtgc agttatcatc ccggcaaacg tcccatgccg aaaagataac cctgtccgaa
    28141 atatgactat ttcagcctaa cattacaatc gtaattaatt cacctataag tattttttgg
    28201 catgtaagtt gctttattta ctgtgtaagc aatgaaatca gccgcgacgc aaccgggttg
    28261 caccaccaca aatcagcggt agcaccaaaa aggtgcgatt taagaatttt gcttcggaat
    28321 ggtgcggcgc atctcgcaaa aacaaacaat aaaacagcaa tatctttaca tttagccaaa
    28381 ctaaatgtta cctaacaagc acaacactgc acaataaagt tgcagatgat aacaacacaa
    28441 acactcacaa cgggtatcca tgcgttctta acgcagaaga taaaggagtt ggatatgcaa
    28501 ttacgtaaac ctgccacagc aatcctggcc ctggcgcttt ccgcaggact ggcacaggca
    28561 gatgacgccg ccccggcagc aggcagtacg ctggacaaaa tcgccaaaaa cggcgtgatt
    28621 gtcgtcggtc accgtgaatc ttcagtacct ttctcttatt acgacaatca gcaaaaagtg
    28681 gtgggctact cgcaggatta ctccaacgcc attgttgaag cagtgaaaaa gaaactcaac
    28741 aaaccagact tacaggtgaa attgattccg atcacctcac aaaaccgtat tccactgctg
    28801 caaaacggca ctttcgattt tgaatgcggt tctaccacca acaatgccga acgccaaaaa
    28861 caggcggctt tctctgacac tattttcgtg gtcggtacgc gcctgctgac caaaaagggc
    28921 ggcgatatca aagattttgc cgacctgaaa ggcaaagccg tagtcgtcac ttccggcacc
    28981 acctctgaag ttttgctcaa caaactgaat gaagagcaaa aaatgaatat gcgcatcatc
    29041 agcgccaaag atcacggtga ctctttccgt accctggaaa gcggtcgtgc cgttgccttt
    29101 atgatggatg atgctctgct ggccggtgaa cgtgcgaaag cgaagaaacc agacaactgg
    29161 gaaatcgtcg gcaagccgca gtctcaggaa gcctatggtt gcatgttgcg taaagacgat
    29221 ccgcagttca aaaagctgat ggatgatacc atcgctcagg tgcagacctc cggtgaagcg
    29281 gaaaaatggt ttgataagtg gttcaaaaat ccaattccgc cgaaaaacct gaacatgaat
    29341 ttcgaactgt cagacgaaat gaaagcactg ttcaaagaac cgaatgacaa ggcactgaac
    29401 taattacaag aaccaggggc ggaaattcca gccctctcga ttgttacgta gcacggacag
    29461 actatacgcc tggtggtcgt tccccatcgg gcctgaaaac cgcaatacgc tgggtaacaa
    29521 tcttcgaggg tagcagttaa cgctgctacc ctcttttttc tggagtagat ttatgtctat
    29581 agactggaac tggggtattt ttttacaaca agccccgttc ggcaacacca cctatcttgg
    29641 ttggatctgg agtggttttc aggtgacgat cgctttgtcg atctgcgcct ggattatcgc
    29701 tttcctcgtc ggttcatttt tcggcatttt acgtaccgtt ccaaaccgtt ttctctccgg
    29761 tctgggtacg ttgtatgtcg aactgttccg caacgtgccg ttgattgtgc aattctttac
    29821 ctggtacctg gtgatcccgg agctgctgcc agagaaaatc ggcatgtggt ttaaggcaga
    29881 gctggatccc aatattcagt ttttcctttc atccatgctc tgcctggggc tgtttaccgc
    29941 cgcccgtgtt tgcgaacagg tacgcgccgc tattcagtcg ctgccgcgtg ggcaaaaaaa
    30001 tgccgcgctg gcgatgggtc tgacgctacc gcaggcttac cgctacgtac tgctgcctaa
    30061 tgcttatcgc gttatcgtcc cgccgatgac ctcagagatg atgaacctgg tgaaaaactc
    30121 cgccatcgcc tcgactatcg gtctggtgga tatggcggcg caggcgggta aattgctcga
    30181 ttactcggcc cacgcctggg agtcgtttac cgccatcacg ctggcgtatg tattgattaa
    30241 cgcttttatc atgctggtga tgacgctggt tgaacgtaaa gttcgtctgc ccggcaacat
    30301 ggggggcaaa taatgtacga gtttgactgg agttccattg ttccttccct gccatatctg
    30361 ctcgacgggc tggtgatcac gctgaaaatc accgtcacgg cagtggtgat tggtattttg
    30421 tggggcacca tgctggcggt gatgcgcttg tccagctttg cgcctgttgc gtggtttgcc
    30481 aaagcctatg ttaacgtatt ccgctcgatt cctttagtca tggttttgct gtggttttac
    30541 ctgattgtgc cgggatttct gcaaaacgtg ctgggattat cgccaaaaaa tgatatccgt
    30601 ttaatttcgg caatggtggc gttttcgatg tttgaagcgg cctactattc agagattatc
    30661 cgtgccggta ttcaaagtat ttcccgtggt cagtcgagcg ccgcgctggc gttggggatg
    30721 actcactggc agtcgatgaa actgattatt ctgccgcagg ctttccgcgc gatggtgccg
    30781 ctgctgctca ctcagggcat cgtactgttc caggatacct cgctggtgta tgtgttatct
    30841 ctggccgatt tcttccgtac cgcttcaacc attggtgagc gtgatggtac gcaggttgag
    30901 atgatcctgt ttgccggatt tgtttatttc gttattagcc ttagcgcgtc gttgttggtc
    30961 agctacttga aaagaaggac agcataatga ttaccctgaa aaatgtttca aaatggtatg
    31021 gtcactttca ggtgctgacc gactgctcaa ccgaagtgaa aaaaggcgaa gtggtggtgg
    31081 tttgcggccc gtctggttcc ggcaaatcaa cgctgattaa aaccgtcaac ggcctcgaac
    31141 cggtgcagca aggtgaaatc accgtcgatg gcatcgtggt taacgacaag aaaaccgatc
    31201 aggcaaagct gcgttcccgc gtcgggatgg tattccagca tttcgagctg ttccctcatc
    31261 tgtcgattat cgaaaacctg accctggcgc aggtgaaagt gcttaaacgc gataaagcgc
    31321 cggctcgtga aaaagcattg aaattgctgg agcgcgtcgg gctttccgca catgccaata
    31381 agtttccggc gcagctttcc ggcggtcagc agcagcgtgt ggcgatcgct cgcgcattgt
    31441 gtatggatcc tattgccatg ctgttcgacg aaccgacatc ggcgctggat ccggagatga
    31501 tcaacgaagt gctggacgtg atggtggaac tggcgaacga aggaatgacc atgatggtgg
    31561 tgacccacga aatgggcttt gcccgtaaag tggcgaatcg ggtgatcttt atggacgagg
    31621 gtaaaattgt cgaagactcg ccgaaagacg ctttcttcga tgatccgaaa tcggaccgcg
    31681 caaaagactt cctcgcgaaa atcctgcatt aatcactctg gcgcgcatac ccaagtgcgc
    31741 gccatctcac gctttatcgt ttcgctcctc gtatctgtta catcctggcg gtacccttgt
    31801 cgcaaagaag cacacaacaa ggagcaacaa tggcactgcc aattctgtta gattgcgacc
    31861 caggtcatga cgacgctatc gcaatagttc ttgccctcgc ctcaccagag cttgatgtca
    31921 aagcaattac gtcttccgcc ggaaaccaga caccagaaaa aaccttacgc aatgttctgc
    31981 gtatgctgac cttgcttaat cgcaccgata ttccggtagc aggcggcgcg gtaaaaccgt
    32041 taatgcgtaa tttgattatt gcggacaatg tgcacggcga aagcggtctc gacggcccgg
    32101 cattaccgga accgacgttc gcaccgcaaa actgtacggc ggtggagctg atggcgaaaa
    32161 cgctgtgtga aagtgaggaa cctatcacca ttgtttcaac cggaccgcaa actaacgttg
    32221 ccttgctgct caatagccac ccggaactgc atagcaaaat tgcccgtatc gtgattatgg
    32281 gcggcgcaat ggggcttggt aactggacgc ctgcggctga atttaatatt tacgttgacc
    32341 cggaagcggc agaaattgtc ttccagtcag gaattccggt ggtgatggcc ggtctggatg
    32401 ttactcacaa agcacaaatc cacgttgaag acactgagcg tttccgcgcg attggcaacc
    32461 ctgtttcaac cattgttgcc gaactgctgg atttcttcct cgaatatcat aaagacgaaa
    32521 aatggggctt tgtcggcgca ccactgcatg acccatgcac catcgcctgg ctgttgaaac
    32581 cggagttgtt taccactgtt gagcgctggg ttggcgtgga aacacaaggg aaatataccc
    32641 agggtatgac ggttgttgat tattattatc tgactggcaa taaaccgaat gccaccgtaa
    32701 tggtcgatgt tgatcgtcag ggctttgttg atttactggc tgatcgtctg aaattttacg
    32761 cttaatcact cgccggatga catcacgcca tccggcacca ctcatttcat tacggctcga
    32821 acggacgtct ctggaactga tcatgaccac atttcgggca tagcgtcagc acttccggtg
    32881 tatagatcgg gagatggaag tgacatttct cgcagaccag atttcccagc cctaccactt
    32941 ctccgctgtg ataaaccccg tgatgattga ggtcctggaa aacttcgcgc cattcaagct
    33001 gcgttttatc ggtgatgtct gccagctcct gccacaagct ttctttaatc acccgcataa
    33061 agacgctgtc agattcttct ttcaggctct cttcatagct catggcgaac tcttccaggt
    33121 cacgtctgac agctcgcgtc agctcatcga cctcggttcg cgttaactcc cctgttttta
    33181 ttacgcgctc gcgcgcctgt tccaccagtg cgtcgatatc acgttcgcca ttgcgcaggc
    33241 gttcgctcag tgacgcgacc agttcacggt aatattgagc aaccttgttc atcgtttcat
    33301 ctcccgggtc gttaactgac tataataata gacgttcttt gttccttgct ttgctaatac
    33361 gcccacaaac tctgtaaatt aatgtatgaa taatccgggc atcatttcaa cgtcgagtgc
    33421 gcgaaaggct gttttgacgc ggacgtttgg gctatgctat gcggatctga aaaaccacct
    33481 caacgctaca tttgtagccg tattgaaaac aggaccactg gctgccatgc aagagcaata
    33541 ccgcccggaa gagatagaat ccaaagtaca gcttcattgg gatgagaagc gcacatttga
    33601 agtaaccgaa gacgagagca aagagaagta ttactgcctg tctatgcttc cctatccttc
    33661 tggtcgacta cacatgggcc acgtacgtaa ctacaccatc ggtgacgtga tcgcccgcta
    33721 ccagcgtatg ctgggcaaaa acgtcctgca gccgatcggc tgggacgcgt ttggtctgcc
    33781 tgcggaaggc gcggcggtga aaaacaacac cgcaccggca ccgtggacgt acgacaacat
    33841 cgcgtacatg aaaaaccagc tcaaaatgct gggctttggt tatgactgga gccgcgagct
    33901 ggcaacctgt acgccggaat actaccgttg ggaacagaaa ttcttcaccg agctgtataa
    33961 aaaaggcctg gtatataaga agacttctgc agtcaactgg tgcccgaacg accagaccgt
    34021 actggcgaac gaacaagtta tcgacggctg ctgctggcgc tgcgatacca aagttgagcg
    34081 taaagagatc ccgcaatggt ttatcaaaat caccgcttac gctgacgagc tgctcaacga
    34141 tctggataaa ctggatcact ggccagacac cgttaaaacc atgcagcgta actggatcgg
    34201 tcgttccgaa ggcgtggaaa tcaccttcaa cgttaacgac tatgacaaca cgctgaccgt
    34261 ttacactacc cgcccggaca cctttatggg ttgtacctac ctggcggtgg ctgcgggtca
    34321 tccgctggca cagaaagcgg cggaaaataa tcctgaactg gccgccttta ttgacgaatg
    34381 ccgtaacacc aaagttgccg aagctgaaat ggcgacgatg gagaaaaaag gcgtcgatac
    34441 tggctttaaa gcggttcacc cattaacggg cgaagaaatt cccgtttggg cagcaaactt
    34501 cgtattgatg gaatacggca cgggcgcagt tatggcggtt ccgggtcacg accagcgcga
    34561 ctacgagttt gcctctaaat acggcctgaa catcaaaccg gttatcctgg cagctgacgg
    34621 ctctgagcca gatctttctc agcaagccct gactgaaaaa ggcgtgctgt tcaactctgg
    34681 cgagttcaac ggtcttgacc atgaagcggc cttcaacgcc atcgccgata aactgactgc
    34741 gatgggcgtt ggcgagcgta aagtgaacta ccgcctgcgc gactggggtg tttctcgtca
    34801 gcgttactgg ggcgcgccga ttccgatggt gacgctggaa gacggtaccg taatgccgac
    34861 cccggacgac cagctgccgg tgatcctgcc ggaagatgtg gtgatggacg gcattaccag
    34921 cccgattaaa gcagatccgg agtgggcaaa aactaccgtt aacggtatgc cagcgctgcg
    34981 tgaaaccgac actttcgaca cctttatgga gtcctcctgg tactatgcgc gctacacttg
    35041 cccggagtac aaagaaggta tgctggattc caaagcggct aactactggc tgccggtgga
    35101 tatctacatt ggtggtatcg aacacgccat tatgcacctg ctctacttcc gcttcttcca
    35161 caaactgatg cgtgatgcag gcatggtgaa ctctgacgaa ccagcaaaac agttgctgtg
    35221 tcagggtatg gtgctggcag atgccttcta ctatgttggc gaaaacggcg aacgtaactg
    35281 ggtttccccg gttgatgcta tcgttgaacg tgacgagaaa ggccgtatcg tgaaagcgaa
    35341 agatgcggca ggccatgaac tggtttatac cggcatgagc aaaatgtcca agtcgaagaa
    35401 caacggtatc gacccgcagg tgatggttga acgttacggc gcggacaccg ttcgtctgtt
    35461 tatgatgttt gcttctccgg ctgatatgac tctcgaatgg caggaatccg gcgtggaagg
    35521 ggctaaccgc ttcctgaaac gtgtctggaa actggtttac gagcacacag cgaaaggtga
    35581 tgttgcggca ctgaacgttg atgcgctgac tgaagatcag aaagcgctgc gtcgcgatgt
    35641 gcataaaacg atcgctaaag tgaccgatga tatcggccgt cgtcagacct tcaacaccgc
    35701 aattgcggcg attatggagc tgatgaacaa actggcgaaa gcaccgaccg atggcgagca
    35761 ggaccgcgcc ctgatgcagg aagcactgct ggccgttgtc cgtatgctta acccgttcac
    35821 cccgcacatc tgcttcacgc tgtggcagga actgaaaggc gaaggcgata tcgacaacgc
    35881 gccgtggccg gttgctgacg aaaaagcgat ggtggaagac tccacgctgg tcgtggtgca
    35941 ggttaacggt aaagtccgtg caaaaatcac cgttccggtg gacgcaacgg aagaacaggt
    36001 tcgcgaacgt gctggccagg aacatctggt agcaaaatat cttgatggcg ttaccgtgcg
    36061 taaagtgatt tacgtacctg gtaaactcct caatctggtc gttggctaag cgcgggagga
    36121 agcgtgcgat atctggcaac attgttgtta tctctggcgg tgttaatcac cgccgggtgt
    36181 ggctggcatc tgcgtgatac cacgcaggtt ccttccacta tgaaggtcat gatcctggac
    36241 tcaggcgatc cgaacgggcc attaagccgt gcggtgcgta accagttacg tctaaatggt
    36301 gtcgagttgc ttgataaaga aaccacgcgt aaggacgttc catccctgcg tttgggtaaa
    36361 gtgagcatcg cgaaagatac cgcatcggta ttccgtaacg gtcaaacagc agagtatcag
    36421 atgatcatga cggtcaatgc gaccgtgttg atccccggcc gtgacatcta cccgattagc
    36481 gccaaagtct tccgttcgtt cttcgataac ccgcaaatgg cgttagcgaa agataacgaa
    36541 caagacatga tcgtaaaaga gatgtacgac cgtgctgccg aacagctgat tcgtaagctg
    36601 ccaagcatcc gtgctgcgga tattcgttcc gacgaagaac agacgtcgac cacaacggat
    36661 actccggcaa cgcctgcacg cgtctctacc acgctgggta actgatgatt cggttgtacc
    36721 cggaacaact ccgcgcgcag ctcaatgaag ggctgcgcgc ggcgtatctt ttacttggta
    36781 acgatcctct gttattgcag gaaagccagg acgctattcg tcaggtagct gcggtacaag
    36841 gattcgaaga acaccacact ttttccattg atcccaacac tgactggaat gcgatctttt
    36901 cgttatgcca ggccatgagt ctgtttgcca gtcgacaaac gctattgctg ttgttaccag
    36961 aaaacggacc gaatgcggcg atcaatgaac aacttctcac actcaccgga cttctgcatg
    37021 acgacctgct gttgatcgtc cgcggcaata aattaagcaa agcgcaagaa aatgccgcct
    37081 ggtttactgc tcttgcgaat cgcagcgtgc aggtgacctg tcagacaccg gagcaggctc
    37141 agcttccccg ctgggttgct gtgcgcgcaa aacagctcaa cttagagctg gatgacgcgg
    37201 cgaatcaggt gctctgctac tgttatgaag gtaacctgct ggcgctggct caggcactgg
    37261 agcgtttatc gctactctgg ccagacggca aactgacatt acctcgcgtt gaacaggcgg
    37321 tgaatgatgc cgcgcatttc accccttttc attgggttga tgctttgttg atgggaaaaa
    37381 gtaagcgcgc gttgcatatt cttcagcaac tgcgtctgga aggcagcgaa ccggttattt
    37441 tgttgcgcac attacaacgt gaactgttgt tactggttaa cctgaaacgc cagtctgccc
    37501 atatgccact gcgtgcgttg tttgataagc atcgggtatg gcagaaccgc cggggcatga
    37561 tgggcgaggc gttaaatcgc ttaagccagc cgcagttacg tcaggccgtg caactcctgg
    37621 cacgaacgga actcaccctc aaacaagatt acggtcagtc agtgtgggca gagctggaag
    37681 ggttatctct tctgttgtgc cataaacccc tggcggacgt atttatcgac ggttgatatg
    37741 aaatctttac aggctctgtt tggtggcacc tttgatccgg tgcactatgg tcatcttaaa
    37801 cccgtggaaa cgctggcgaa tttgattggt ctgacacggg tcacaatcat ccctaataat
    37861 gttcctccgc atcgtcccca gccggaagcg aacagcgtac agcgtaaaca catgcttgaa
    37921 ctggcgattg ccgacaagcc attatttact cttgatgaac gcgagctaaa gcgcaatgcc
    37981 ccctcttaca ctgcgcaaac actgaaagag tggcggcagg aacaagggcc ggacgtgccg
    38041 ctggcgttta ttataggtca ggattcattg ctgacctttc cgacctggta cgaatacgaa
    38101 acgatactcg acaatgcaca tttgatcgtc tgtcggcgtc caggttaccc acttgaaatg
    38161 gcgcaaccgc aataccagca atggctggaa gatcatttga cacataaccc ggaagatctt
    38221 caccttcagc ctgccggtaa aatttatctg gctgaaacgc cgtggtttaa catctcggcg
    38281 accatcatcc gcgaacgttt gcaaaacggt gaatcgtgtg aggatttatt gccggaaccg
    38341 gtactgactt acattaacca acaaggcttg tatcgctgat acctgtcgtt acggagggca
    38401 tgatgcgact gtggttaatt cgtcatggcg aaacgcaagc gaatgtcgat ggtctgtaca
    38461 gcggtcatgc gcccactccc ctgaccgcgc gcggtattga gcaagcgcaa aatctgcata
    38521 cgctgctaca tggtgtttcc tttgacctgg ttttatgcag tgaactggaa cgggcacagc
    38581 ataccgcgcg acgggttctc agtgatcgcc agctccccgt gcaaatcatc cctgaactca
    38641 acgaaatgtt ttttggcgac tgggagatgc gacatcatcg cgacctcatg caagaagatg
    38701 ccgaaaacta tagcgcgtgg tgcaatgact ggcagcatgc tatccccacg aacggtgaag
    38761 gatttcaggc attttcacaa cgtgtggaac ggtttatcgc aaggcttagt gaatttcagc
    38821 actatcagaa tattttagtc gtcagccatc agggtgtact gagtctgtta atcgctcgtt
    38881 taattggcat gcctgccgag gctatgtggc attttcgcgt tgaccagggg tgttggagcg
    38941 ccattgatat caaccaaaaa ttcgcgacgc tacgcgtcct caatagccgt gccatcgggg
    39001 tcgagaatgc atgacttttc tgttttttta cgggtaagcc gcaacgacca ttgacagccc
    39061 cgggcaggct gatattctcc gcagccagac ttttttcgcc agacacgact ttgtagaaat
    39121 tgttttacaa aaatggcgat gcaatctgcg gcgcggggtg ggatgatagc ccactttcga
    39181 aagccgattc ggcgacaatt gtcccgaaat cgcctctggt tcaggtatac tgacagacca
    39241 tttttatcta tttgattcac ccagggggaa aacttgcagg gtaaagcact ccaggatttt
    39301 gttatcgaca aaattgatga cctcaaaggt caggacatca tcgccttaga cgttcagggc
    39361 aaatccagca tcaccgactg catgatcatc tgtacgggta cgtccagccg ccatgttatg
    39421 tccattgctg atcacgttgt gcaggagtct cgcgcagcgg gcctgttacc gctcggcgta
    39481 gaaggtgaaa acagcgccga ctggattgtc gtggatttgg gcgatgtgat tgtccatgtc
    39541 atgcaggaag agagccgtcg cctgtatgaa ctggaaaaac tctggagtta atgcgtgaag
    39601 ctgcaacttg tcgccgtggg aacgaaaatg ccggactggg tacaaaccgg ttttaccgag
    39661 tacctgcgtc gttttccgaa agatatgccc ttcgagctga ttgaaattcc ggccggaaaa
    39721 cgcggcaaga atgcggacat caagcgcata ctcgacaaag agggtgagca gatgttggcg
    39781 gccgcaggca aaaaccgcat tgtcaccctc gatattccag gcaagccctg ggatacgccg
    39841 cagttagccg ctgagctgga acgctggaag ctggatggtc gcgacgtcag tctactgatt
    39901 ggcgggcctg aagggttgtc gcctgcctgt aaagcggcgg cggagcagag ctggtcgctg
    39961 tcggcgctta ccctccccca tccgctggtt cgcgtgctgg tcgcagagag tctgtaccgg
    40021 gcgtggagca tcaccaccaa ccatccttat caccgtgagt gataagggag ctttgagtag
    40081 aaaacgcagc ggatgaaact acagaactct tttcgcgact atacggcaga gtccgcgctg
    40141 tttgtgcgcc gggcgctggt cgcctttttg gggattttgc tgctgaccgg cgtgcttatc
    40201 gccaacctgt ataatctgca aattgttcgc tttaccgatt accagacccg ctctaatgaa
    40261 aaccgcatta agctggtgcc tatcgcgccc agccgcggca ttatctacga ccgtaacggt
    40321 atccctctgg ccctcaaccg cactatctac cagatagaaa tgatgccgga gaaagtcgat
    40381 aacgtgcagc aaacgctgga cgctttgcgc agcgtggttg atctgaccga tgacgatatt
    40441 gctgcattcc gaaaagagcg cgcacgttca caccgtttca cctccattcc ggtgaaaact
    40501 aacctgaccg aagtacaagt agctcgcttt gccgtcaatc agtaccgttt tccgggtgtt
    40561 gaagttaaag gctataaacg tcgttactat ccttacggtt cagcgttgac ccacgtcatc
    40621 ggctatgtgt cgaaaatcaa cgataaagac gtcgaacgcc tgaataatga cggcaaactg
    40681 gccaactatg cggcaacgca tgatatcggt aagctgggca ttgagcgtta ctatgaagac
    40741 gtgctgcacg gtcagaccgg ttatgaagag gttgaagtta acaaccgtgg gcgtgttatc
    40801 cgccagttaa aagaagtgcc accgcaagcc ggacacgata tttacctgac gctggatctc
    40861 aaactccagc aatatatcga aacgctgctg gcgggtagcc gcgcagctgt ggtagtcacc
    40921 gatccgcgta caggtggggt gctggcgctg gtttccacgc ctagttatga cccaaacctg
    40981 tttgttgacg gtatctccag caaagattat tccgccttgc tgaacgaccc gaatacaccg
    41041 ctggtgaacc gcgccacaca gggggtttat cctcccgcgt ctacagttaa accctatgtg
    41101 gcggtttcgg cattgagcgc cggagtgatc acgcgcaata cgacgctgtt tgaccctggc
    41161 tggtggcaac tgccagggtc ggaaaaacgt tatcgtgact ggaaaaaatg gggccacggg
    41221 cgtctgaatg tcacaagatc gctggaagaa tctgcggata ccttcttcta tcaggtggcc
    41281 tacgatatgg gaatcgatcg cctctccgaa tggatgggta aattcggtta tggtcattac
    41341 accggtatcg acctggcgga agaacgttcc ggcaacatgc ctacccgcga atggaaacag
    41401 aaacgcttta aaaaaccgtg gtatcagggt gacaccattc cggttggtat cggtcagggt
    41461 tactggacag cgaccccaat ccagatgagt aaggcactga tgatcctgat taatgacggt
    41521 atcgtgaagg ttccgcattt gctgatgagc accgccgaag acggcaaaca ggtgccatgg
    41581 gtacaaccgc atgaaccgcc cgtcggcgat attcattccg gttactggga gctggcaaaa
    41641 gacggtatgt acggtgttgc taaccgccct aacggtactg cgcataaata ctttgccagc
    41701 gcaccgtaca aaattgcggc gaaatctggt acagctcagg tcttcggtct gaaagcgaac
    41761 gaaacctata atgcgcacaa aattgccgag cgtttacgtg accacaaact gatgaccgcc
    41821 tttgcgccat acaacaatcc gcaagtcgct gtcgccatga ttctggagaa cggcggtgcg
    41881 ggtccggcgg ttggtacgct gatgcgccag atcctcgacc acattatgct gggtgataac
    41941 aacaccgatc tgcctgcgga aaatccagcg gttgccgcag cggaggacca ttaatcatga
    42001 cggataatcc gaataaaaaa acattctggg ataaagtcca tctcgatccc acaatgctgc
    42061 tgatcttact ggcattgctg gtttacagcg ccctggttat ctggagcgcc agcggtcagg
    42121 atattggcat gatggagcgt aaaataggcc aaatcgcgat gggcctggtc atcatggtgg
    42181 tgatggcgca aattcctcca cgcgtttatg aaggctgggc cccctatctc tatatcatct
    42241 gtattatttt gctggtggcg gtagatgctt ttggtgccat ctctaaaggc gctcaacgct
    42301 ggctggacct cggtattgtt cgttttcagc cgtcggaaat tgccaaaata gccgtaccac
    42361 tgatggttgc gcgctttatc aaccgcgacg tttgcccgcc atcgttgaag aacactggca
    42421 tcgcgctggt gctgatattt atgcccacgc tgctggtggc tgcacagcct gacctgggaa
    42481 catcaatcct cgttgcgctt tccggcctgt ttgtactgtt cctctctggc cttagctggc
    42541 gtctgattgg cgtcgcagta gtgctggtag cggcgttcat tccgattctg tggttcttcc
    42601 tgatgcatga ttaccagcgc cagcgcgtaa tgatgctcct ggacccggaa tcagacccac
    42661 tcggcgcagg ctatcacatt attcagtcta aaattgctat tggctccggc ggattacgcg
    42721 gcaaaggctg gctgcacggc actcagtcac agcttgaatt tctccccgaa cgccataccg
    42781 actttatctt cgcggtactg gcggaagagc tgggattagt gggcattttg attctgctcg
    42841 ctctctacat tctgctgatc atgcgcgggc tgtggatagc cgccagagcg caaaccacct
    42901 ttggtcgcgt catggctggc ggcttaatgc tgatattatt cgtttatgtc ttcgtaaata
    42961 ttggtatggt aagcggtatt ctgccggttg taggggttcc gctcccactg gtcagttatg
    43021 gaggatcggc gctaattgtg ctgatggctg ggttcgggat tgtaatgtca atccacaccc
    43081 acaggaaaat gttgtcgaaa agcgtgtaag aggtgcgcaa tgcgtaagca gtggctcggg
    43141 atctgcatcg cggcaggaat gctcgcggca tgtacaagcg atgatggtca gcaacagacg
    43201 gtaagtgtac cgcagcctgc ggtatgtaac ggccccatag ttgaaattag cggggcggac
    43261 ccgcgtttcg aaccactgaa tgcgacggca aatcaggatt accagcgcga cggtaaaagc
    43321 tacaaaatcg tgcaggatcc gtctcgattt agccaggcgg gactggcggc aatctatgat
    43381 gccgaacccg gcagtaacct gacggcctct ggcgaagctt tcgatccgac acagttgacg
    43441 gcggcccatc caacgcttcc gatccccagc tacgccagaa tcactaacct ggctaacggg
    43501 cgaatgatcg tggtgcgcat taatgatcgc ggtccttacg gcaacgaccg cgttatttcg
    43561 ctttctcgcg cggcagctga ccgtcttaac acgtcaaaca acaccaaagt tcgtatcgat
    43621 ccgattattg tcgcccagga tggttcgctt tctggtcctg gtatggcttg taccacagtt
    43681 gccaaacaga cttacgccct gcctgcacct cccgatttaa gcggtggcgc gggaacaagt
    43741 tcagtgtctg gcccgcaggg tgacattctt ccggtcagta attcgacgct aaaaagcgaa
    43801 gatccgaccg gcgcgccggt aaccagcagc ggtttcctcg gcgcaccaac gaccttagcg
    43861 cctggtgtac tggaaggcag cgaaccgacg cctgcgccac agcccgttgt tacagctccg
    43921 tcgacaacgc ctgcaacctc acctgcaatg gtgacaccgc aagccgcctc gcaaagcgcc
    43981 agcggcaact ttatggtgca agtcggggcc gttagcgatc aggctcgtgc gcaacagtac
    44041 caacagcaac tgggacagaa gttcggcgtc cccggtcgcg taactcaaaa tggcgcggtc
    44101 tggcggatcc agcttggccc attcgccagc aaagccgaag ccagtacctt gcagcaacgt
    44161 ttgcaaaccg aagcccaatt acagtcattt attaccaccg cgcagtagcg taaagcaggc
    44221 atctgatgtg tcaatttctg taaatgacgt taacaaagtc acgcggaaag tcagatgcct
    44281 gccggtagtg catttgctat agtagggcac tttttttaat tccatcacgg atgtcgtagt
    44341 tcagaccatg aataccattt tttccgctcg tatcatgaag cgcctggcgc tcaccacggc
    44401 tctttgcaca gcctttatct ctgctgcaca tgccgatgac ctgaatatca aaactatgat
    44461 cccgggtgta ccgcagatcg atgcggagtc ctacatcctg attgactata actccggcaa
    44521 agtgctcgcc gaacagaacg cagatgtccg ccgcgatcct gccagcctga ccaaaatgat
    44581 gaccagttac gttatcggcc aggcaatgaa agccggtaaa tttaaagaaa ctgatttagt
    44641 caccatcggt aacgacgcat gggccaccgg taacccggta tttaaaggtt cttcgctgat
    44701 gttcctcaaa ccgggcatgc aggttccggt ttctcagctg atccgcggta ttaacctgca
    44761 atcgggtaac gatgcttgtg tcgccatggc cgattttgcc gctggtagcc aggacgcttt
    44821 tgttggcttg atgaacagct acgttaacgc actgggcctg aaaaataccc acttccagac
    44881 ggtacatggt ctggatgctg atggtcagta cagctccgcg cgagatatgg cgctgatcgg
    44941 ccaggcgttg atccgtgacg taccgaatga atactcgatc tataaagaaa aagaatttac
    45001 gtttaacggt attcgccagc tgaaccgtaa cggtctgtta tgggataaca gcctgaatgt
    45061 cgacggcatc aaaaccggac acactgacaa agcaggttac aaccttgttg cttctgcgac
    45121 tgaaggccag atgcgcttga tctctgcggt gatgggcgga cgtactttta aaggccgtga
    45181 agccgaaagt aaaaaactgc taacctgggg cttccgtttc ttcgaaaccg ttaacccact
    45241 gaaagtaggt aaagagttcg cctctgaacc ggtttggttt ggtgattctg atcgcgcttc
    45301 gttaggggtt gataaagacg tgtacctgac cattccgcgt ggtcgcatga aagatctgaa
    45361 agccagctat gtgctgaaca gcagtgaatt gcatgcgccg ctgcaaaaga atcaggtcgt
    45421 cggaactatc aacttccagc ttgatggcaa aacgatcgaa caacgcccgc tggttgtgct
    45481 gcaagaaata ccggaaggta acttcttcgg caaaatcatt gattacatta aattaatgtt
    45541 ccatcactgg tttggttaaa aattgaacac ttgaaagtgt gatttccgtc cccatatact
    45601 aagcatcagt aaaaaactcc cgccttctgg cgggagttgc tatttaatta cgttacgccg
    45661 gagctgacat gaaaaccaaa cttaacgaac tgcttgaatt ccctactcct tttacttaca
    45721 aagttatggg gcaggcgtta cctgagctgg ttgatcaggt ggttgaagtg gtacagcgcc
    45781 atgcgccagg tgactacacc ccaacggtaa aaccaagcag caaaggcaac taccactcgg
    45841 tatctatcac tatcaacgct actcatatcg agcaggttga aacactgtat gaagaactgg
    45901 gcaaaatcga tattgtccgc atggttctgt aactcgcttc tccgttaccc ggctcctggt
    45961 cgggtaactc ccctctcctc gctgtgatat acttttcccc cacttttact cattctccac
    46021 ggagatgccg ttttgtatca ggataaaatt cttgtccgcc agctcggtct tcagccttac
    46081 gagccaatat cccaggctat gcatgaattc accgataccc gcgatgatag tacccttgat
    46141 gaaatctggc tggtcgagca ctatccggta ttcacccaag ggcaggcagg aaaagcggag
    46201 cacattttaa tgccgggtga tattccggtg atccagagcg atcgcggtgg gcaggtgact
    46261 tatcacgggc cggggcaaca ggtgatgtat gtgttgctta acctgaaacg ccgtaaactc
    46321 ggtgtgcgtg aactggtgac cttgcttgag caaacagtgg tgaataccct gtctgaactg
    46381 ggtatagaag cgcatcctcg ggctgacgcg cctggtgtct atgtcgggga aaagaaaatt
    46441 tgctcactgg gtttacgtat tcgacgcggt tgttcattcc acggtctggc attaaacgtc
    46501 aatatggatc tttcaccatt tttacgtatt aatccttgtg ggtatgcagg aatggaaatg
    46561 gctaaaatat cacaatggaa acccgaagcg acgactaata atattgcacc acgtttactg
    46621 gaaaatattt tagcgctact aaacaatcct gacttcgaat atattaccgc ttaattccat
    46681 acatcaatgg cccaatttac actgggtcat tactcataac acctgctaat gctttacatc
    46741 tccttcgcgt ttgtatattt tctttatcac ttgcagaaat gcgatgctac gccttgctcc
    46801 gtagcccacc taattgatta acccattgaa ataattgacc atggtgagca aaatcgttcc
    46861 gtcccaatta gacataagat tattgtaatt tttttaaata ttatttttcc atgaataaaa
    46921 ttggagtaag tgcgtggata gtaataatca aattgaaacc tgtttaagca gaaagtcgtc
    46981 agaaggtaaa ccacaaatat ttacaacctt acgaaatatt gatcttaacc ttctgactat
    47041 ttttgaagct gtatatgtac ataaagggat cgttaatgca gcgaaagtgc ttaatctgac
    47101 accctcggca atcagtcagt ctattcagaa actgcgcgtt atattccctg acccattgtt
    47161 tattcgcaaa ggccagggtg tcactcctac cgcatttgcg atgcatctac atgagtatat
    47221 cagtcagggc cttgagtcca ttcttggcgc gctggatatc gaaggaagct atgataagca
    47281 acgaacgata actattgcga ccaccccctc agtcggagcc ctggtccttc ctgtcatcta
    47341 tcgggcgatt aaaactcact atccgcagct tttactgcgc aacccgccca tcagcgacgc
    47401 ggaaaaccaa ctcagtcagt ttcaaaccga tctcatcatc gataacatgt tttgcaccaa
    47461 tcgtacggtg caacatcatg ttctgttcac cgacaatatg gtgttaattt gccgtgaggg
    47521 aaatccacta ctctctttag aagatgacag ggagactatc gacaacgctg cgcatatact
    47581 cctgttaccg gaagggcaaa atttcagcgg tctgcggcag agagttcaag agatgtttcc
    47641 ggaccggcaa atcagtttca ccagctacaa cattttgaca atcgctgcac tggttgccaa
    47701 cagtgacatg ttagcgatta ttcccagccg tttttataac ctgtttagcc gttgttggcc
    47761 gctggaaaaa ctgccttttc cgtccttaaa tgaggagcaa atagatttct ctatccacta
    47821 caacaaattc agcctgcgtg atcccatcct gcacggggta atagatgtta tccgtaatgc
    47881 attttgaaaa agtattgtta cagccagatc acagcgaaga gcgaaaaaat ggcacaatcc
    47941 aacaacaatt ttacattttc gcgaccgctt tggctgcttt caggtccgtt tcaatgatat
    48001 actgccagtc gttaattcaa aaatagttga taattacaac aatctattga attgaaacgc
    48061 tttccttcgt aattcgcaac tggaacacgc acgctatgag taaacccatt gtgatggaac
    48121 gcggtgttaa ataccgcgat gccgataaga tggcccttat cccggttaaa aacgtggcaa
    48181 cagagcgcga agccctgctg cgcaagccgg aatggatgaa aatcaagctt ccagcggact
    48241 ctacacgtat ccagggcatc aaagccgcaa tgcgcaaaaa tggcctgcat tctgtctgcg
    48301 aggaagcctc ctgccctaac ctggcggaat gcttcaacca cggcacagca acgtttatga
    48361 tcctcggcgc tatttgtacc cgccgttgtc cgttctgtga cgttgcccac ggtcgcccgg
    48421 tagctccgga tgccaatgaa ccagtgaaac tggcgcagac cattgccgat atggcgctgc
    48481 gttatgtggt tatcacctcc gttgaccgtg atgacctacg cgatggcggt gcccagcact
    48541 ttgcggattg cattactgcc attcgggaaa aaagcccgca aatcaaaatt gaaactctgg
    48601 tgccggattt ccgcggtcgt atggatcgtg ctctggatat tctgaccgcc acaccaccag
    48661 atgtgttcaa ccataacctg gaaaacgtac cgcgtattta ccgtcaggta cggcctggtg
    48721 cagattacaa ctggtcgctg aagctgctgg aacgctttaa agaagcgcat ccggaaatcc
    48781 cgaccaagtc tggtctgatg gtgggtctgg gtgaaaccaa tgaagaaatt attgaggtaa
    48841 tgcgcgacct gcgccgtcat ggtgtgacga tgttaacgct ggggcaatat ttgcagccaa
    48901 gccgccatca cctgccggtt caacgttacg ttagcccgga tgagttcgac gaaatgaaag
    48961 ccgaagcgct ggcgatgggc tttacccatg ctgcatgcgg tccgtttgtc cgctcgtctt
    49021 accacgccga tttgcaggcg aaagggatgg aagttaagta atcctgtaat aattacttac
    49081 atctgtctga taaaaaaccc gctttttgaa gcgggttttt tgtatcgaac aagatattga
    49141 gggagcgtcc tgctcgccac gtcactcttt atgagagagc ttttcagcct gaagatcaac
    49201 gtctgcgcct tttttcgccg cagcatcgtc atcattcatc gccttcttga accctttaat
    49261 ggccgctcca aggtctccgc ccagcgtacg taacttctta gtcccaaaca gcagaacgac
    49321 cagcgccgca actaccagca gtttggtaat actaatctca cccatagata ccttcttgac
    49381 atataaccgg caacagaacg cgccttaaaa accagagaat attaacggtc attcgacgcg
    49441 cgcacacaat agcaatctgt aacaaggtga atcaagcgcc gagtaaaaaa acatcataat
    49501 aattgcggcg gcgcaaagcg acggttgttt aaaacgggaa gttgcgcgcg cacctgacgc
    49561 acacgttcgg gcgtcacttc cgccataatg agtgcaggca tttctgacgc tgccgcaatg
    49621 gtgacgccaa agggatctat aattcggctt tgcccgatat ttttgttccc gcactccccc
    49681 gccgccacca tataacaggt ggtatccagc gcacgagcgg caagcaacgt tgaccagtga
    49741 tgctctttga gcggcccacg aacccaggcg gcaggaagta ccaggatttc agctccctgt
    49801 aatgcctgtg ccagcgccag ctctggaaag cgtaagtcat aacaggtcat cagaccgacc
    49861 ttcatccctt ccacctccag taacggagct atttcattac cagcatcaac atggcgtgat
    49921 tcctgaatgg caaatgcatc atagagatgc agtttggcat aacgggcgac gatgtttcct
    49981 gcctgtagcg ccaccagcat attccatgcg cgccccggcg ttgaaggaac atgaatcgtc
    50041 agaattgtcg tcatcatgtt acgtttactt tctcgccgta aacgtccgag gaattcgcct
    50101 tccagcagct gtgctgattt aaccgataga tctgcatcat gatcatcgcg cgccaacaat
    50161 gcttccggca ggacaaacag cgaaacgtcg ttttccgccg cctgcgccat caacgaggca
    50221 caaatctcag cgttcttttc ccacacagat gtaacagcaa actgtcctgc ggcaaccagc
    50281 attgccacct cctcctgtca gagtcgtaaa attcgccaac ggcgattatt cggcgttaca
    50341 cttatcactc atacaaatca aatagcagga ttttgcagtg ttacaacttc ttttagcagt
    50401 ttttattggc ggtggtacgg gaagcgtggc gagatggctg ttaagtatgc gatttaaccc
    50461 gctgcatcaa gcgattccgt tggggacgct ggctgcaaac ctgattgggg cattcatcat
    50521 aggaatggga ttcgcctggt ttagcaggat gacgaacatt gatccagtgt ggaaagtatt
    50581 aatcaccacc ggattttgtg gcggtctaac aaccttctca acattttcgg cagaagtggt
    50641 gtttttgtta caagagggcc gctttggctg ggcgttactg aacgttttcg tcaacctttt
    50701 ggggtctttt gccatgaccg cactggcttt ctggctgttt tccgcctcaa ccgcacacta
    50761 aagaccaaaa aaaacccgct gattaagcgg gttttgaatt cttgctgacg tatcttacag
    50821 agcgattacg tttgcagcag aagggccttt ggcaccgtta gtgatttcga actctacgcg
    50881 ctgaccttca gcaagagttt taaaaccatt agtctggatt gcagagaagt gtacgaacac
    50941 gtctttgctg ccgtcttccg gagtaatgaa accgaatcct ttggactcat taaaccactt
    51001 aacgttacct ttaatcttag acatcaaaat tacctttaca tgaaaaatag acacaaatgc
    51061 tgtgtcggtt accagtacac caattgtggt acgctttgtc cagtgcaact ttactaaaaa
    51121 gtgataaaag tcgcgttatt tttgatgtta attattttac tcattgattt taaagagttt
    51181 actaaaattt catttgtctc aaaactgaaa gcgcatccag gcaaagtaca cattgccatt
    51241 gttataggta cccggaatgt aggtcatctg aaaagtcact ggaccataac ccacagaggc
    51301 caatggcagt agaaccggga gagggatgta attccagtta tcgcgtgccg ttacgccagc
    51361 ggtgaatccc agtcctaaat gaaaattttc atctgccagc ggtcgccagg tactttccca
    51421 tccgtatccg gcaatcggtt cccatttgtt ccacgagtcc ttaaatgcca tggcatacag
    51481 gccatgccag tttccttttt catcccaacg cgactgacca aaaccgccac cccacggtcg
    51541 ctcgttatag cgatcggttt tttctttgtc gtaagcgaaa cgtgcatgcc aggtgatggc
    51601 aggaatatat aaatcataat gttcaggctg ttgccaggtt tgtgcaatat tttctctaaa
    51661 cgttgtcatc cactcatctg cgttagcaaa aactttacca acgctgatta actgaacaaa
    51721 aacaaaggaa aagatagcga catatttact cacgttcatt tgtgaccata aaacatttat
    51781 caaaaatcta ctactagcat agcaaagcta caattaacat aaccttaata gccccaacat
    51841 aaagaataat ctgaatagct gctctattta tttccgcttg tttaagttaa atatttccgc
    51901 gcatcgatta aagatgatgc gtcgttttct gcgatgggaa tagtcaaaaa agaaa
//
LOCUS       DAPHCV010000016        51675 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.016, whole genome shotgun sequence.
ACCESSION   DAPHCV010000016 DAPHCV010000000
VERSION     DAPHCV010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.016"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            324..1130
                     /gene="xthA"
                     /locus_tag="RH033_000959"
     CDS             324..1130
                     /gene="xthA"
                     /locus_tag="RH033_000959"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDV2990183.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALMTKETPIAVRRGFPGDDEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            1297..2007
                     /locus_tag="RH033_000960"
     CDS             1297..2007
                     /locus_tag="RH033_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDV2990184.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFELLTDLPHLLTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            2012..2689
                     /gene="ydjY"
                     /locus_tag="RH033_000961"
     CDS             2012..2689
                     /gene="ydjY"
                     /locus_tag="RH033_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HDV2990185.1"
                     /translation="MLQHYSVSWKKGLTALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVAGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            2704..3411
                     /locus_tag="RH033_000962"
     CDS             2704..3411
                     /locus_tag="RH033_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDV2990186.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            3411..3968
                     /locus_tag="RH033_000963"
     CDS             3411..3968
                     /locus_tag="RH033_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDV2990187.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLVLEYAEAASVTPPTVDDALRARLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPENVLKER"
     gene            3968..5134
                     /locus_tag="RH033_000964"
     CDS             3968..5134
                     /locus_tag="RH033_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV2990188.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNDAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPTALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWRKGKDFPPSPARMDALLKANTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRESLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            5107..6642
                     /locus_tag="RH033_000965"
     CDS             5107..6642
                     /locus_tag="RH033_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2990189.1"
                     /translation="MATPLRYALIFLLWAMMAVIYAPLIPAVLTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHMA
                     FATSALLLFADGGLLYDYFPYFTPPMDRFGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLGYLLWRGWRRTIPRVDGVRKP
                     ATPLLPGNTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVAAFIALLLLL
                     LWLEWGPQRRRLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGYLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRSKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            6642..7295
                     /locus_tag="RH033_000966"
     CDS             6642..7295
                     /locus_tag="RH033_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDV2990190.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGGLAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRAQAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            7362..8669
                     /gene="ynjE"
                     /locus_tag="RH033_000967"
     CDS             7362..8669
                     /gene="ynjE"
                     /locus_tag="RH033_000967"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HDV2990191.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSANWLDKMSTEQLNEWIKQHNLKADAPVALY
                     GNDKDVDAVKKRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(8678..9298)
                     /locus_tag="RH033_000968"
     CDS             complement(8678..9298)
                     /locus_tag="RH033_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDV2990192.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLMGFAIGVLALP
                     FLALGWYLAALIVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYAMVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGSET
                     ILLFVLSCLFPACFPWFAWVFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            9385..9792
                     /gene="nudG"
                     /locus_tag="RH033_000969"
     CDS             9385..9792
                     /gene="nudG"
                     /locus_tag="RH033_000969"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HDV2990193.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELNEELGIEATVGDYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(9758..10030)
                     /locus_tag="RH033_000970"
     CDS             complement(9758..10030)
                     /locus_tag="RH033_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HDV2990194.1"
                     /translation="MSRALIAVVLAFPLITLANPHYHPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            10266..11609
                     /gene="gdhA"
                     /locus_tag="RH033_000971"
     CDS             10266..11609
                     /gene="gdhA"
                     /locus_tag="RH033_000971"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDV2990195.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLSQGVI"
     gene            complement(11686..13641)
                     /gene="topB"
                     /locus_tag="RH033_000972"
     CDS             complement(11686..13641)
                     /gene="topB"
                     /locus_tag="RH033_000972"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDV2990196.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTSVRQFRGIMAPGGGE
                     GKKKDSPRKRAPKKSPPSEEAGNGVIT"
     gene            complement(13646..14689)
                     /gene="selD"
                     /locus_tag="RH033_000973"
     CDS             complement(13646..14689)
                     /gene="selD"
                     /locus_tag="RH033_000973"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDV2990197.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(14806..15357)
                     /locus_tag="RH033_000974"
     CDS             complement(14806..15357)
                     /locus_tag="RH033_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HDV2990198.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSALLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            15518..17374
                     /gene="sppA"
                     /locus_tag="RH033_000975"
     CDS             15518..17374
                     /gene="sppA"
                     /locus_tag="RH033_000975"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDV2990199.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKTLTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADISITKALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            17539..18555
                     /gene="ansA"
                     /locus_tag="RH033_000976"
     CDS             17539..18555
                     /gene="ansA"
                     /locus_tag="RH033_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDV2990200.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            18566..19207
                     /gene="pncA"
                     /locus_tag="RH033_000977"
     CDS             18566..19207
                     /gene="pncA"
                     /locus_tag="RH033_000977"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HDV2990201.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTTLDDWLRDHEIDELIIMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQE"
     gene            complement(19368..19640)
                     /locus_tag="RH033_000978"
     CDS             complement(19368..19640)
                     /locus_tag="RH033_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HDV2990202.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARNNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(19682..20095)
                     /gene="msrB"
                     /locus_tag="RH033_000979"
     CDS             complement(19682..20095)
                     /gene="msrB"
                     /locus_tag="RH033_000979"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDV2990203.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            20437..21432
                     /gene="gapA"
                     /locus_tag="RH033_000980"
     CDS             20437..21432
                     /gene="gapA"
                     /locus_tag="RH033_000980"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDV2990204.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            21516..22400
                     /locus_tag="RH033_000981"
     CDS             21516..22400
                     /locus_tag="RH033_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HDV2990205.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFHVGKTCEIDLEAHGEFETT
                     SALHTYFNVGDITKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(22451..23305)
                     /gene="yeaE"
                     /locus_tag="RH033_000982"
     CDS             complement(22451..23305)
                     /gene="yeaE"
                     /locus_tag="RH033_000982"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HDV2990206.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALIGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQKQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(23395..24141)
                     /gene="mipA"
                     /locus_tag="RH033_000983"
     CDS             complement(23395..24141)
                     /gene="mipA"
                     /locus_tag="RH033_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HDV2990207.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            24577..26511
                     /gene="yeaG"
                     /locus_tag="RH033_000984"
     CDS             24577..26511
                     /gene="yeaG"
                     /locus_tag="RH033_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HDV2990208.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            26624..27907
                     /locus_tag="RH033_000985"
     CDS             26624..27907
                     /locus_tag="RH033_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HDV2990209.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            28054..29529
                     /locus_tag="RH033_000986"
     CDS             28054..29529
                     /locus_tag="RH033_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDV2990210.1"
                     /translation="MIRATRISLGLFFNYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MASMGTQKLKAQGFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            29710..31200
                     /locus_tag="RH033_000987"
     CDS             29710..31200
                     /locus_tag="RH033_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDV2990211.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     RADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            31243..31746
                     /gene="yeaK"
                     /locus_tag="RH033_000988"
     CDS             31243..31746
                     /gene="yeaK"
                     /locus_tag="RH033_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HDV2990212.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGADFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(31747..31851)
                     /locus_tag="RH033_000989"
     CDS             complement(31747..31851)
                     /locus_tag="RH033_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2990213.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            32021..32467
                     /locus_tag="RH033_000990"
     CDS             32021..32467
                     /locus_tag="RH033_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDV2990214.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(32424..33245)
                     /gene="nimR"
                     /locus_tag="RH033_000991"
     CDS             complement(32424..33245)
                     /gene="nimR"
                     /locus_tag="RH033_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HDV2990215.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKEPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            33342..34523
                     /gene="nimT"
                     /locus_tag="RH033_000992"
     CDS             33342..34523
                     /gene="nimT"
                     /locus_tag="RH033_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HDV2990216.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAIIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWRGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            34578..34925
                     /locus_tag="RH033_000993"
     CDS             34578..34925
                     /locus_tag="RH033_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HDV2990217.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLANIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(34947..35201)
                     /gene="yoaF"
                     /locus_tag="RH033_000994"
     CDS             complement(34947..35201)
                     /gene="yoaF"
                     /locus_tag="RH033_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HDV2990218.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            35384..36409
                     /gene="dgcP"
                     /locus_tag="RH033_000995"
     CDS             35384..36409
                     /gene="dgcP"
                     /locus_tag="RH033_000995"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HDV2990219.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            36442..36540
                     /locus_tag="RH033_000996"
     CDS             36442..36540
                     /locus_tag="RH033_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HDV2990220.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            36543..36617
                     /gene="yoaJ"
                     /locus_tag="RH033_000997"
     CDS             36543..36617
                     /gene="yoaJ"
                     /locus_tag="RH033_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HDV2990221.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(36676..36924)
                     /locus_tag="RH033_000998"
     CDS             complement(36676..36924)
                     /locus_tag="RH033_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDV2990222.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(37072..37254)
                     /locus_tag="RH033_000999"
     CDS             complement(37072..37254)
                     /locus_tag="RH033_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HDV2990223.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(37258..37617)
                     /gene="yeaR"
                     /locus_tag="RH033_001000"
     CDS             complement(37258..37617)
                     /gene="yeaR"
                     /locus_tag="RH033_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HDV2990224.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSTEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(37790..38428)
                     /gene="leuE"
                     /locus_tag="RH033_001001"
     CDS             complement(37790..38428)
                     /gene="leuE"
                     /locus_tag="RH033_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HDV2990225.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(38555..39478)
                     /gene="dmlR"
                     /locus_tag="RH033_001002"
     CDS             complement(38555..39478)
                     /gene="dmlR"
                     /locus_tag="RH033_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HDV2990226.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            39581..40666
                     /gene="dmlA"
                     /locus_tag="RH033_001003"
     CDS             39581..40666
                     /gene="dmlA"
                     /locus_tag="RH033_001003"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HDV2990227.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKKIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            40917..42527
                     /gene="yeaV"
                     /locus_tag="RH033_001004"
     CDS             40917..42527
                     /gene="yeaV"
                     /locus_tag="RH033_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HDV2990228.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAATLSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            42559..43683
                     /gene="yeaW"
                     /locus_tag="RH033_001005"
     CDS             42559..43683
                     /gene="yeaW"
                     /locus_tag="RH033_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HDV2990229.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELIDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            43739..44704
                     /gene="yeaX"
                     /locus_tag="RH033_001006"
     CDS             43739..44704
                     /gene="yeaX"
                     /locus_tag="RH033_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HDV2990230.1"
                     /translation="MSDYQMFEVQVSQIEPLTEQVKRFTLVATDGKPLPAFTGGSHVI
                     VQMSDGDNEYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKLGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHLAELQYSDVDWQLHYCTRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLNIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(44751..45866)
                     /gene="rnd"
                     /locus_tag="RH033_001007"
     CDS             complement(44751..45866)
                     /gene="rnd"
                     /locus_tag="RH033_001007"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HDV2990231.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGISDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEIVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQALPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(45948..47633)
                     /gene="fadD"
                     /locus_tag="RH033_001008"
     CDS             complement(45948..47633)
                     /gene="fadD"
                     /locus_tag="RH033_001008"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HDV2990232.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHLGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(47838..48419)
                     /gene="yeaY"
                     /locus_tag="RH033_001009"
     CDS             complement(47838..48419)
                     /gene="yeaY"
                     /locus_tag="RH033_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HDV2990233.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(48458..49153)
                     /gene="tsaB"
                     /locus_tag="RH033_001010"
     CDS             complement(48458..49153)
                     /gene="tsaB"
                     /locus_tag="RH033_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDV2990234.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGADLPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDEKGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLTLCDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(49211..51121)
                     /locus_tag="RH033_001011"
     CDS             complement(49211..51121)
                     /locus_tag="RH033_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDV2990235.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALTGGDLPVQILSDVILLRSWSNQTIDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            51254..51598
                     /locus_tag="RH033_001012"
     CDS             51254..51598
                     /locus_tag="RH033_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDV2990236.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDTTIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT        12600 a        13232 c        12835 g        13008 t
ORIGIN      
        1 actggaaatc aaggagttat aaccaaacca tatgcattta aagtgcatat aaagtgaata
       61 cgtttgcgat gtgggtgaat aaaaagaata aaaaacgcaa tgttatgcag aagtaaaata
      121 taattctgga attgtgatca ttgacgaaat ttactggaaa ttactgcgcc attctgacgc
      181 aacgcgcacc aaaagcgggc attttttgcg ccatcgttga catcattaac aaccatcgat
      241 caaatcactt aacaacaggc ggtacgcaaa gcgaaattct gctaccatcc acgcactctt
      301 tatctgaata aatggcagcg actatgaaat ttgtctcttt taatatcaac ggcctgcgcg
      361 ccagacctca ccagcttgaa gccatcgtcg aaaagcacca accggatgtg attggcctgc
      421 aggagacaaa agttcatgac gatatgtttc cgctcgaaga ggtggcgaag ctcggctaca
      481 acgtgtttta tcacgggcag aaaggccatt atggcgtggc gctgatgacc aaagagacgc
      541 cgattgccgt gcgtcgcggc tttcccggtg acgacgaaga ggcgcaacgg cggattatta
      601 tggcggaaat cccctcgccg ctgggcaatg tcaccgtgat caacggttac ttcccgcagg
      661 gtgaaagccg cgaccatccg ataaaattcc cggcaaaggc gcagttttat cagaatctgc
      721 aaaactatct ggaaaccgaa ctcaagcgtg aaaatccggt gctgattatg ggcgatatga
      781 atatcagtcc tggcgacctg gatattggca ttggcgaaga gaaccgtaag cgctggctgc
      841 gtaccggaaa atgttctttc cttccggagg aacgcgaatg gatggagaga ctgatgagct
      901 ggggattggt tgataccttc cgccatgcga atccgcaaac agccgatcgt ttctcatggt
      961 ttgattaccg ctcaaaaggt tttgacgata accgtggtct gcgcatcgac ctgcttctcg
     1021 ccagccaacc gctggcagaa tgttgcgtag aaaccggcat cgactatgaa atccgcagca
     1081 tggaaaaacc gtccgatcac gcccccgtct gggcgacctt ccgccgctaa tttagctgtt
     1141 ctcctggctc aaactgggtc aggagaatta accttgagaa aaatcaacaa actgtcagta
     1201 atgatttgtt gcctgccgtc ctttgttata ccgtcactgc gtttttagtt gtctgaccac
     1261 ttctctatta tcaagtttga tataggaaac tccacgatga acgctgagcg taaatttctt
     1321 tttgcctgtc ttatttttgc gctggtcatt tacgctatcc acgctttcgg tttattcgag
     1381 ctgctcaccg atttacctca cttactgaca ctcattcgcc agagcggatt gttcggttat
     1441 agcctctata ttctgttatt catcattgcc accctctttc tgttaccagg aagcatactg
     1501 gtgatcgctg gaggaatagt ttttggcccg ctcttaggga cactactatc attaattgcc
     1561 gccacgctgg cctcctcgtg ctcattcctg ctggcgcgct ggctgggacg tgatttactg
     1621 ctgaaatacg ttggccatag ccataccttt caggccattg aaaaaggcat tgcgcgtaac
     1681 ggtattgatt ttcttattct gacccgctta atcccgttgt ttccttacaa tattcaaaat
     1741 tacgcttacg gattaaccac aatcgccttc tggccttata cccttatttc agcactcacg
     1801 actctgcccg gtattgttat ttataccgtg atggcaagcg atctcgccaa tgaaggcatt
     1861 acgctgcgct ttattttaca actctgtctg gcgggcctgg cgctgtttat tctcgtccag
     1921 ctcgcaaaac tctacgcccg acacaaacat gtggatctgt ctgcttcgcg ccgcagccca
     1981 cttactcacc ccaaaaatga aggatagaac gatgttgcaa cattattcag tgtcatggaa
     2041 aaaaggactg actgcactct gcttactggc tgttgctggg cttagcggct gcgatcaaaa
     2101 agagaatgcg gcggcaaaag tggaatacga cggactttcg aacagccaac cgctgcgtgt
     2161 cgatgccaat aaccatacgg taaccatgct ggtgcaaatt aatggtcgtt ttctcaccga
     2221 cgacactcgt cacggtattg tgtttaaaga tggctccaac ggacataaat cgctgtttat
     2281 gggttatgcg accccgaaag cattttatga agccctgaaa gaggcaggtg gtacaccggg
     2341 cgaaaacatg acgatggata ataaagaaac gacccacgtc gcaggtagca aactggatat
     2401 ttcggtcaac tggcaagggg cggcaaaagc gtattccttc gatgaagtga ttgttgacag
     2461 taatggcaag aaactggaca tgcgctttgg cggtaattta acggcagcag aagagaagaa
     2521 aacgggctgc ctggtgtgtc tggatagctg cccggtcggc atcgtcagca atgcaacata
     2581 cacttatggt gcggttgaaa aacgaggtga agttaaattc aaaggcaatg cctcggttct
     2641 cccggcggat aacacgctgg caacggttac ctttaaaatc accgaataaa gccaggataa
     2701 aggatgatga agatgcaatc gcgaaaaatc tggtactacc gtataaccct catcatcctg
     2761 ttgttcgcta tgctgctggc atgggcgctg cttcccggcg tccatgagtt tatcaatcgc
     2821 agcgttgcgg cgtttgccgc cgtggaccaa cagggtatag aacgctttat tcagtcttac
     2881 ggcgcactag cggcagttgt ctcgttcttg ttgatgattt tacaggccat tgctgcaccg
     2941 ctacctgcgt ttttgatcac ctttgccaat gcgtcgctgt ttggcgcgtt ctggggcggc
     3001 ttgctgtcgt ggaccagttc gatggcgggc gctgcgctgt gcttttttat cgccagagtg
     3061 atgggccgcg aagtggtgga aaaattaacc ggcaaaaccg tacttgacag tatggacggc
     3121 tttttcactc gctacggcaa acacaccatc ctggtctgtc ggttattgcc ttttgtccct
     3181 ttcgatccaa tcagctatgc tgccggtttg acttcaatac gttttcgctc gttttttatc
     3241 gccaccgggc ttggtcaatt accggcgact attgtttatt cctgggcggg cagcatgtta
     3301 acaggcggaa ctttctggtt tgtcaccgga ctgtttattc tgtttgccct gaccgtggtg
     3361 atttttatgg cgaagaaaat atggcttgag cgccagaaga ggaatgcctg atgggtttac
     3421 cgccgcttag caaaattcct ttcattttac gtccgcaggc gtggctgcat cgtcgccatt
     3481 acggcgaggt gctaagcccc attcgctggt gggggcgaat tccgtttatc ttttatctgg
     3541 tgtcgatgtt tgtcggctgg ctggagcgca aacgctcacc gctggatccg gtagtgcgat
     3601 cgcttgtcag cgcgcgcatt gcgcaaatgt gtctgtgtga gttttgcgtg gatatcacca
     3661 gtatgaaagt cgccgagcgc actggcagca ccgataaact gctggcagtg gctgactggc
     3721 ggcaaagccc gctctttagc gatgaagaac ggctggtgct ggagtacgcc gaagccgcaa
     3781 gcgtaacgcc gccaacggtc gatgatgccc tgcgcgcccg actggctgcg cattttgacg
     3841 cccaggcgct caccgaactg acggcattga tcggcctgca aaacctgtca gcccgtttta
     3901 attctgccat ggacattccc gctcaggggc tgtgccgtat tcctgaaaac gttcttaagg
     3961 agagatgatg cgccattgtg ggtggttgct gggattgtta tcgctgtttt ctttggcaac
     4021 acatgccagt gactggcagg aaatcaaaaa cgacgccaaa gggcaaaccg tctggtttaa
     4081 cgcctggggc ggcgataccg caattaaccg ctatctcgac tgggttagcg gcgagatgaa
     4141 aacccattac gctataaacc tgaagattgt tcgtctggcg gatgccgcag acgcggtgaa
     4201 gcgcattcag accgaagccg cagccggacg taaaacgggc ggctcggtgg atctgctctg
     4261 ggtgaacggc gaaaacttcc gcaccttaaa agaggccaat ttattacaaa cgggctgggc
     4321 ggagactctg cccaactggc gctatgtcga cacacagctt ccggtgcggg aagatttttc
     4381 agtgcctaca gaaggggctg aatcgccctg gggcggcgca caactgacgt ttatcgcccg
     4441 ccgcgatgtt acgccacagc caccacaaac gccgcaagcc ttactggagt ttgctaaagc
     4501 caatcccggc acggttacct atccgcgccc accggacttt accggcacgg cgtttcttga
     4561 acagttgctg attatgctaa cgccagatcc cacagcatta aaagaagcgc cggacgatgc
     4621 gactttcgcc cgcgtcactg cgcccttgtg gcaatatctt gatgcactgc atccgtattt
     4681 gtggcgcaaa ggaaaggatt tcccgccttc gcccgcgcgg atggatgctc tgctgaaagc
     4741 taacacattg cgcctgtcgc tgacctttaa ccctgcacat gcgcagcaaa aaatcgccag
     4801 cggcgatttg cctgcaagca gttacagttt tggttttcgc gaggggatga ttggcaacgt
     4861 ccatttcgtc accattcctg ccaacgcgaa tgccagtgct gcggcgaagg tagttgccaa
     4921 tttcctgctc tcacccgatg cgcaactgcg taaagcagat cccgctgtct ggggcgatcc
     4981 ttctgttctc gatccgcaaa aactgcctga cgggcagcgc gaatcattgc aatcgagaat
     5041 gccgcaggat ctgccgccgg tactggctga accgcacgca ggttgggtaa atgcgctgga
     5101 acaagaatgg ctacaccgtt acggtacgca ttaatctttt tgctgtgggc gatgatggcg
     5161 gtgatttatg caccgctgat cccggcagtt ctcacgctga tatcgcctgc tttgtcgttg
     5221 acgcactggc aggcgttatt tgccgatcca cagttaccgc aggcattact ggcaacgctg
     5281 gtgtcgacaa ccattgcggc ggtcggggcg ttgttgattg ccctgctggt gattgtggcg
     5341 ttgtggccag ggccgaaatg gcagcgtatg tgcgcccggc tgccgtggct gctcgccatt
     5401 ccccatatgg cttttgccac cagcgccctt ctgctctttg ctgacggagg gctgctttat
     5461 gactatttcc cgtatttcac tccgccaatg gaccgatttg gcatcggtct gggcctcacc
     5521 ctcgcagtga aagaaagcgc atttctgctg tggatcttag cggcagtatt gagcgaaaaa
     5581 cggctgttgc agcaagttat tgtactggat tcgctgggct acagccgctg gcaatgcctg
     5641 aactggctgc tgttgccctc tgtcgcgcct gcgctggcaa tggcgatgct ggcgattgtg
     5701 gcctggtcgc tgtcggtcgt ggatgtggcg attatccttg ggccaggtaa tcccccgacg
     5761 ctggcggtaa ttagctggca gtggttaact cagggcgacg ccgatcaaca aacgaaaggt
     5821 gcactcgcca gcctgctgct gatgctgtta ctcgccgcct acgttttgct gggctatctg
     5881 ctatggcgtg gctggcggcg tactattccc cgcgtagatg gcgttcgtaa gcctgccacg
     5941 cctttattgc caggcaatac gctggcgagt tttttaccct taaccggtgt gctgtgtgtg
     6001 gttctgctgg cgatcctcgc ggatcagtcg acgatcaata gtgaagcgct catcaacagc
     6061 ctgacaatgg ggctggtggc ggcattcatc gctttgctcc tgttactgct gtggctggaa
     6121 tgggggccac aacgtcgccg gttgtggcta tggttgccca ttttattacc cgctctgccg
     6181 ctggtagcgg gccagtacac gctggcgcta tggctaaacc tggatggaag ctggacggcg
     6241 gtggtctggg ggtatctgct gtgggtaatg ccgtggatgc tgtttatcct gcaacccgcc
     6301 tggcagcgca ttgattcacg gttaattttg attgcgcaaa cactgggctg gtcgcggtcc
     6361 aaaatcttct tttacgtgaa atgtccgctt atgctacgcc ctacgctgat tgccttcgcg
     6421 gtgggatttt cagtcagtat tgcacagtat atgccgacgc tgtggctggg cgcggggcgt
     6481 tttccgacgc tcaccactga agcggtggca ttaagcagcg gcggcagcaa cggcattctc
     6541 gccgcccagg ctttatggca actgctatta ccgcttatta tttttgccct gaccgcgtta
     6601 gtcgcaaaat gggtaggtta tgtcagacaa ggactccgct aatgctctgc gtgaaaaatg
     6661 tttcgctacg tttaccagaa agccgcttgc tgacaaacgt taactttacg gtggataaag
     6721 gtgacattgt cacgttaatg gggccgtctg gctgtggaaa atccactctg ttttcatgga
     6781 tgattggtgg actggccgga cagttttctt gtacagggga gctatggctc aatgagcaac
     6841 ggattgacat gctacccacc gcacagcgtc agattggcat tcttttccag gatgcactgt
     6901 tatttgacca gttcagcgtc gggcaaaatt tactgctggc gctaccggcg acacttaaag
     6961 ggaatgcccg acgtaatgcc gtgaatgatg cacttgagcg ttcaggcctt gacggagctt
     7021 tccatcagga tcctgctact ttgtctggcg gtcagcgagc gcgcgttgct ctgctacgcg
     7081 cccttctcgc ccagccaaaa gcgttactcc tggatgagcc attcagccgt ctagatgtgg
     7141 ctctgcgcga taattttcgc cagtgggtgt tcagcgaagt tcgcgcccag gcgatccccg
     7201 tcgttcaggt aacgcacgat ctccaggatg ttcctgctga tagttctgtt ctggatatgg
     7261 cgcagtggtc agaaaattac aacaaactgc gataacgcaa agtttttctc aatgcgtcag
     7321 ttcagaatgg cgcactcaaa actacaatgt cgggattttc gatgaaacgt gtttctcaaa
     7381 tgaccgcgct ggcaatggct ttagggctgg cttgcgcttc ttcgtgggcc gctgaactgg
     7441 cgaagcctct tacacttgac cagcttcaac aacaaaatgg caaagcgata gatactcgcc
     7501 ccagcgcgtt ttataacggc tggccacaaa ccttaaatgg cccttctggt catgaacctg
     7561 ccgccttaaa cctctctgct aactggctcg acaaaatgag caccgaacag ctcaacgagt
     7621 ggatcaagca acataacctg aaagccgatg ctccggtggc gctgtacggt aatgacaaag
     7681 atgtcgacgc cgtcaaaaag cgactgcaaa aagcaggttt tacgcatatc tccattctga
     7741 gtgacgcgct aagcgaacct tcccgtctgc aaaaactgcc gcattttgag cagctggttt
     7801 atccgcaatg gctgcacgac ctgcaacaag gtaaagaggt tacggcgaaa cctgccggtg
     7861 actggaaagt cattgaagcg gcctggggcg ctcctaagct ttaccttatc agccatattc
     7921 ccggcgctga ctacatcgat accaacgaag tggaaagcga accgctgtgg aacaaagttt
     7981 ctgatgaaca actaaaagcg atgctggcaa aacacggcat tcgccatgac accacggtca
     8041 ttctgtacgg gcgtgacgta tacgctgcag cgcgtgtggc acaaattatg ctctacgctg
     8101 gcgtgaaaga tgtccgcctg ctggacggcg gctggcaaac ctggtccgac gcgggactgc
     8161 ccgttgagcg cggaacgcca ccgaaagtga aagcggaacc ggatttcggc gtgaagatcc
     8221 cggcacaacc gcaactgatg cttgatatgg aacaggcgcg tggactgctg catcgccagg
     8281 atgcctcgct ggtgagcatt cgttcgtggc cagaatttat cggtacgacc agcggttaca
     8341 gctatattaa accaaaaggt gaaatagccg gagcacgttg gggacacgct ggtagcgact
     8401 cgacgcatat ggaagatttc cataacccgg atggcaccat gcgtagcgcc gatgatatta
     8461 ccgccatgtg gaaagcatgg aatatcaagc cagatcagca agtttcattc tactgcggca
     8521 ccggctggcg cgcgtccgaa acctttatgt acgcacgcgc aatgggctgg aataacgtct
     8581 ccgtttatga cggtggctgg tacgaatgga gcagcgatcc aaaaaatccg gtagcaaccg
     8641 gtgaacgcgg cccggacagc agtaaataac attgcaatta ctgacgctgg agcgacttca
     8701 gcgtcagata cccactccac acccgcgtaa aggtcgtcat ccagcacaac gcaccaaata
     8761 cccacgcaaa ccacggaaaa caggccggaa agaggcaact cagaacaaac agcaaaatcg
     8821 tctcgctgcc ttcggttaag ccgcccagat aataaaacga tttatgcgca taaccgggat
     8881 tatcaatctg atgtttcgcc gccagcgcag caaaagcgag aaaactgctg cccgtgccga
     8941 taaacgcaaa cagcagccag cctccggcca gcgcgttttg ctccggtgcg gcgaggataa
     9001 acccaaaagg caccatcgcg taaaaaagaa aatcgagaga aatatcgaga aagccgcccg
     9061 cgtcggtaag ccctctgcgc cgcgccagcg caccgtccag accatcaagc agacgattca
     9121 acaaaatgac gattagcgcc gccagatacc agcccagcgc cagaaaaggc agtgccagca
     9181 cgccgatggc aaatcccatt aatgttaaac catctggcgt aatgcccggt ttatcaagaa
     9241 cccgcacgca ctgatgcaac aacggtttaa tccggggatg aagatggcgg tctagcacag
     9301 gcactcctta aatataaagc ctttccgatt gagcaacagt gcggatatta tggcattttt
     9361 cgcttatctg cccgtgtgta atttatgaaa atgattgaag ttgttgccgc catcattgaa
     9421 cgtgatggca aaattttact cgcgcaacgc cccgcccaga gcgatcaggc gggattatgg
     9481 gagtttgccg gtggtaaagt cgagccggat gaaagccagc ggcaggcgct ggtgcgtgag
     9541 ttaaacgaag aactgggcat cgaagcaact gttggtgact atgttgccag ccatcagcga
     9601 gaagtttcgg ggcggattat ccatcttcat gcctggcacg tacccgactt ccacgggacg
     9661 ttacaggcac atgaacatca ggcgctggtc tggtgctcac ctgaagaggc gctgcaatat
     9721 ccgctggccc ctgctgacat tccattatta gaggcgttta tggctttacg cgccgccaga
     9781 ccagcggatt agtgctaagg gttttgtcat cccgttggca ttgcagcagt attccttcgg
     9841 ctttaattac cgccccttca gaataatttt gatcctgata aacgcagcac tgagtacagg
     9901 gctgcgctga ctgcccgcct gaactgaaca cttctggcgg tacgtttacc tccacgtccg
     9961 gatgataatg cgggttagcc agtgtaatta atggaaatgc taatactacg gcgatcaatg
    10021 ctcgactcac agggaactcc ttaacgttat tgtctcttct acagataacg gtagccgggc
    10081 ggcaaaactt tagcgtctga gttatcgcat ttggttatga gattactctc gttattaatt
    10141 tgctttcctg ggtcattttt ttcttgctta ccgtcacatt cttgatggta tagtcgaaaa
    10201 ctgcaaaagc acatgacata aacaacataa gcataatcgt attaatatat aagggtttta
    10261 tatctatgga tcagacatat tctctggagt cattcctcaa ccatgtccaa aagcgcgacc
    10321 cgaatcaaac cgagttcgcg caagccgttc gtgaagtaat gaccacactc tggccttttc
    10381 ttgaacaaaa tccaaaatat cgccagatgt cattactgga gcgtctggtt gaaccggagc
    10441 gcgtgatcca gtttcgcgtg gtatgggttg atgatcgcaa ccaggtgcag gtcaaccgcg
    10501 catggcgcgt gcaattcagc tccgctatcg gtccgtacaa aggcggtatg cgcttccacc
    10561 cttcagtaaa cctttccatt ctcaaattcc tcggcttcga gcaaaccttt aaaaatgccc
    10621 tgacaaccct gccgatgggc ggtggtaaag gcggcagcga tttcgacccg aaagggaaaa
    10681 gcgaaggcga agttatgcgt ttttgccagg cgctgatgac tgaactgtat cgccacctgg
    10741 gcgcggatac tgacgttccg gcaggtgata tcggcgttgg tggtcgtgaa gtcggcttta
    10801 tggcggggat gatgaaaaag ctctccaaca ataccgcctg cgtcttcacc ggtaagggcc
    10861 tttcatttgg cggcagtctt attcgcccgg aagctaccgg ctacggtctg gtttatttca
    10921 ccgaagcaat gctaaaacgc cacggtatgg gttttgaagg gatgcgagtt tccgtttctg
    10981 gctccggcaa cgtcgcccaa tacgctatcg aaaaagcgat ggaatttggt gctcgtgtga
    11041 tcactgcgtc agactccagc ggcactgtag ttgatgaaag cggattcacg aaagagaaac
    11101 tggcacgtct tatcgaaatc aaagccagcc gcgatggtcg agtggcagat tacgccaaag
    11161 aatttggtct ggtctatctc gaaggccaac agccgtggtc tgtaccggtt gatatcgccc
    11221 tgccttgcgc cacccagaac gaactggatg ttgacgccgc gcatcagctt atcgccaatg
    11281 gcgttaaagc cgtcgccgaa ggggcaaata tgccgaccac catcgaagcg actgaactgt
    11341 tccagcaggc aggcgtactg tttgcaccgg gtaaagcggc taatgctggc ggtgtagcaa
    11401 cgtcaggcct tgaaatggcg caaaacgctg cgcgcctggg ctggaaagcc gagaaagttg
    11461 acgcacgttt gcatcacatc atgctggata tccaccatgc ctgtgttgag catggtggtg
    11521 aaggtgagca aaccaactac gtgcaggggg cgaacattgc cggttttgtg aaggttgcgg
    11581 atgcgatgtt aagccagggc gtgatttaag acaattgtgc cggatacagc gcaaacgcct
    11641 tatccggcca taaaaatctt cagtatcaga tggttgaaag aacctttatg ttatcactcc
    11701 attgcccgcc tcttctgatg gcgggctttt tttcggcgct ctcttgcgtg gcgaatcctt
    11761 tttcttccct tctccaccgc cgggagccat tattccccga aactgcctta ccgacgtgcg
    11821 ctttgcctga tcaatcaact ggtacaatgt ccccaccagc ggctgcataa agtcctgata
    11881 gcgacactgc ttttcgctga tttgcgtcag caccgattcc cagtgcgcgg tcatgtccgg
    11941 tcgcgtcgcc atttctggca gcgaatggaa tagcgctttt ccggcgtcgg tggagtggat
    12001 atagcgccct tttttggtca ggaagcctcg cttgaacagc agttcaataa tcccggcgcg
    12061 agtcgcttct gtccccagac catcggtcgc acgaaggatc tttttcagat ctttgtcctg
    12121 cacaaaacgt gctatcccgg tcatcgctga aagcagtgtt gcatcggtaa aatggcgcgg
    12181 cggctgggtt tgccgctcta ccacttcacc tttttcacac agcaactcat cgcctttcgc
    12241 caccacaggc agcggcgtgc cgtcgttttc ttcatcgcgc tctttgctgc ctaacagtgt
    12301 gcgccagcct gcttcagcaa gaaaacgggc tttagcgaca aatttgcctt tggcaatgtc
    12361 cagttcgata acacacttgc ggaacaccgc atccgggcag aactgcatca aatactgacg
    12421 ggcaatcagg ttatagacct tcgcttcgtt ctccgtcagg ttgatcgcag aactccgtgc
    12481 ggtcgggatg atggcgtggt gcgcatcgac ctttttgtca tcccagcagc ggttgcgtat
    12541 atctggatcg acaactggct gcggtaacaa gtccggtgca tgaacgctga tggcattcat
    12601 cactgcgtgg cgtccggcaa aatgctcttc cggcagatag cgacaatcgg aacgcggata
    12661 agtgattagc ttgtgcgttt cgtacaactt ctggcagata tcaagcacgt tctgcgcact
    12721 cagaccaaag cgttttgccg cttcaatctg caacgccgaa agcgaaaacg gcagcggtgc
    12781 ggattctgat tcccgtttat cgttatagct ggtgacaata gccggttggc cgctgatgcg
    12841 gttgaccaca tgctccgcca gcggacgatg taacaagcgc ccttcttcat cctgataagg
    12901 ttcacacgct tcgctcggtt gccagatagc ggtaaaccgc tcatcggcag gtgtcacgat
    12961 atgcgctttg acttcaaaga aatctttcgc cacgaagttt tcaatctctt cgtcgcggcg
    13021 cactaccagc ccgagcacgg gcgtctgcac gcgtcccacg gaaagtacgc cctgataacc
    13081 cgcattgcga ccgagaattg tgtaagcgcg ggtcatattg atgccgtaca gccagtcagc
    13141 tcgggctcgc gccagtgcag aaacgcacag cggtacaaac tcgctattgg agcgaagacg
    13201 atcgatcgcc cgctcaaccg cctgcgggtt caggtcgttt atcaagcaac gctgtacctg
    13261 ctggcgcttt tccggtgcca gttgcagata gtccagcact tcatccacca gcaattgccc
    13321 ttcgcgatcc gggtctccgg cgtgaacgat ttcgctggct tcatgcagga agcgtttgat
    13381 gacattaagt tgtttagtca cggagggtcg gggctgtaat tgccactttt ccgggacaat
    13441 tggcaaatcc gcaagattcc agcgcgcata gcggctgtcg taggcgtctg gctgcgcctg
    13501 ctcaagcagg tgaccgatac accaggtcac cacctgacca ttaccgcact cgataaagcc
    13561 atcgcctttc cggtgcggtt taggcaggac atcagcaatg gcgcgcgcca gactcggttt
    13621 ttcggcaata aacaaccgca ttgaattaac ggatctcaac catggcacga ccgccgcgcg
    13681 ctggcaccag ttcgccaatc gccgtcagtt caatgccaaa ctcggcggct gtagctttga
    13741 cctcattttc tgcttccggc atgaccgcca gcagcagacc gccagaagtt tgcggatcgc
    13801 acagcagatc gcgcacttca cgcggcattt cacccatcag atgaccgtag ctggcaaagt
    13861 tacgttcggt gccgccaggc actgcgccca acttaatgta ctcttcaacc cccggcagtt
    13921 tcgggatcgc gtcatagtcg acgcgcgcct gcacaccagc cccctgacac atttcgctta
    13981 aatgccccag cagaccaaag cccgtaacgt cggtcatcgc ttttacgcct tcgatgttgg
    14041 caaaggacgc gcctgcgatg ttcatccggc acatcacttc cgtagccagt ccttgatgtt
    14101 ctggtttcag cagtgatttt ttctcagccg tggtaagaac gccaatcccc agcggtttcg
    14161 tcaggaacag tttgcatcca gcttgtgcgg tactgttttt cttcacccgc tcggtcggta
    14221 cgatccccgt caccgccaga ccaaaaatcg gctccggcgc atcgatggag tgaccgccag
    14281 ccagcgcaat ccccgcctga cgacatgcat agcgtccacc ttcggtcact tcgcgggcaa
    14341 tttccgggga aagtttgtta atcggccagc cgaggatcgc aatcgccata atcggtttgc
    14401 cccccatcgc gaagatatcg ctgatggcgt tagtcgccgc aatgcggcca aaatcgaaag
    14461 gattatcaac gatcggcata aagaagtcgg tggtgctgat aacgctggtg ccattgccca
    14521 gatcgtacac cgccgcatcg tcgcgggttt cattacccac aagcaaattc ggatcaacaa
    14581 acttcgcctg ctcactgtgc aggatggttt ccaacacttt tggggaaatt ttacagccgc
    14641 aaccagcgcc gtggctgtat tgggtcaaac gaatcgagtt ctcgctcatg gacatctcct
    14701 gtcaatgcaa tccgggtatg gtaaccctca ttccgtgaag tgataagtga gagtgtctga
    14761 attcctgcgc ctttgctcac aatccagaca gtttcgcgac aattatcaga aataagtgac
    14821 aaacggcgtc gggtccggga cgttaatcga cgtagatgct ttcagctgcg gcgtaccaag
    14881 gtagagaaaa ccgacaattt tatcctgctc acggcaaccg aatgcttcac gcactaccgg
    14941 actttcagtt aatgcgccac tgcgccagat gccgccaaac ccctgggcaa ctgctgccat
    15001 ttgcatcgcc atgaccgcgc atcccgcaga catttcctgt tcccagcgcg ggactttatg
    15061 attttcttcg cattttgcca ccaccgttat gatgagcggt gcgcggaacg gcgcattacg
    15121 cgctttgtcg atagctttgt catcactacc ggcagcaatc gccccctgtt ccaataatgc
    15181 gctgaaacgc tcgcgtcctt ccccttcaat cacaaaaaaa tgccacggtt gcatggactt
    15241 atggtccgga gcacgcatac ccgctcgcag tatgttttgc agttgttcac ccgttggcgc
    15301 aggttcagcc aggcgggagg cgctacggcg attgatcaat agttcgagtg catccatttg
    15361 attaactcct gtcatgatat ttattcacaa aattaacacg agagtggatt ttgttacagc
    15421 ccagtccgca attcctgctg acaagtgccg gttgggtcat tacgataacc acatctattg
    15481 cgcctgaaac aggtgtgacc ttaagttggg agaatacatg cgaacccttt ggcgatttat
    15541 tgccggattt tttaaatgga cgtggcgtct gctgaatttc gtccgtgaaa tggtacttaa
    15601 cctgttcttt atttttctcg tgctggttgg tgtggggatc tggatgcagg tcagtggtgg
    15661 tgattcgaaa gaaacggcca gtcgtggcgc actgctgctg gacatttctg gtgtgatcgt
    15721 cgataaaccc gacagttctc agcggtttag taaattaagc cgccagctgc ttggtgccag
    15781 ttccgatcgt ctgcaggaaa actcactgtt tgatatcgtc aacaccattc gccaggcgaa
    15841 ggacgaccgc aatatcaccg gtattgtgat ggatctgaaa aacttcgcag gcggcgacca
    15901 gccgtctatg cagtacatcg gcaaagctct gaaagagttt cgtgatagcg ggaaaccggt
    15961 ttatgccgtt ggcgagaact acagccaggg gcaatattat ctcgccagtt tcgccaataa
    16021 aatttggctg tctccgcaag gcgtggttga tctgcacggc tttgccacca acggtctgta
    16081 ctacaaatca ttactggata agctgaaagt ttccacccat gtgttccgcg tgggtacgta
    16141 taaatctgcc gttgaaccgt ttattcgtga tgatatgtcg ccggcagccc gcgaagctga
    16201 cagccgctgg attggtgagc tgtggcaaaa ctatctgaat actgttgccg ctaaccgcca
    16261 gatccctgct cagcaggtat tccctggcgc gcaagggttg cttgagggtt taaccaaaac
    16321 cggtggcgat accgcgaaat atgcgctgga aaacaagctg gtcgatgcac tggcatcgag
    16381 tgccgaaatc gaaaaaactc tgaccaaaga gttcggctgg agtaaaactg ataaaaatta
    16441 tcgcgccatc agttattacg attacgcatt gaaaacgccg gcagataccg gtgacagtat
    16501 cggtgtcgtc ttcgctaatg gcgcaattat ggatggcgag gaaactcagg ggaatgttgg
    16561 cggtgatacc actgcggcac aaatccgcga cgctcgcctt gacccgaaag tgaaagcgat
    16621 tgtcctgcgt gttaatagcc caggcggcag cgttaccgcg tctgaagtga ttcgcgctga
    16681 actggctgcc gccagagcag cgggtaaacc tgtggttgtg tcgatgggcg gtatggcggc
    16741 atctggtggt tactggattt ccacgccagc taattacatt gtggctaacc ccagcaccct
    16801 gaccggttct atcggtatct tcggcgtgat cactaccgta gaaaatagtc tggattcgat
    16861 tggtgttcat accgatggcg tctcaacttc accgctggca gatatttcca tcaccaaggc
    16921 actgccgccg gaagcgcagc agatgatgca gttaagcatt gagaatggct ataaacgctt
    16981 tatcacgctg gttgctgatg cgcgtcattc gacgccggag cagattgata aaatcgccca
    17041 gggccacgtc tggaccggtc aggatgcaaa agctaacggg ctggtcgata gtctcggtga
    17101 tttcgatgat gcggtcgcca aagcagcaga gctggcaaaa gtgaaacagt ggcatctgga
    17161 atactacgtt gatgaaccga ccttcttcga caaagtgatg gacaatatgt ctggttctgt
    17221 ccgggcaatg ttgccagatg cgttccaggc catgttacct gcaccgctgg cctcggtagc
    17281 ctctaccgtt aaaagtgaaa gtgacaagct ggcagcgttt aacgacccac aaaaccgtta
    17341 tgcgttttgc ctgacctgcg ccaacgtgcg ttaagtcttg tactgagtgg ccgacagatc
    17401 gtcggccaca tttttttacc tcgacgaatc ctcttcccgc tgtctcgccc catatttcct
    17461 tatccgcata gtatcaggtg cgctccccct gcctcacgta tatacttttg ctctttcgat
    17521 atcattcata tcaatatcat gcaaaagaaa tcaatttacg tcgcctacac gggcgggacc
    17581 atcgggatgc agcgttccga gcagggttat attccggtgt caggtcatct acagcgccaa
    17641 ctggcgctga tgccggaatt ccatcgcccg gagatgccag atttcactat tcatgaatat
    17701 acgccattga tggattcttc tgatatgacg ccagaagact ggcagcatat tgctgaagat
    17761 attaaagcgc actatgacga ctatgatggt tttgtcattc tgcacggcac agacacgatg
    17821 gcgtataccg cctctgcact gtcgttcatg ctcgagaatc tcggtaaacc ggtcattgtg
    17881 acagggtcac aaatcccgct ggctgagtta cgctctgacg gacaaattaa tctgctgaat
    17941 gcgttgtacg ttgcggcgaa ttatcctatc aacgaagtaa ctctcttttt taataatcga
    18001 ctgtatcgtg gcaacagaac caccaaagcc catgccgatg gttttgatgc gtttgcctct
    18061 ccaaaccttc ctccgttact ggaagcaggt atccatattc gccgtttgaa tacgccaccc
    18121 gccccgcacg gtgaagggga attgatcgtt catccaatca cgccgcaacc aattggcgta
    18181 gtgacgattt atccggggat ttctgctgac gtcgtgcgca attttctgcg ccaacctgtg
    18241 aaagcattga ttctgcgctc atatggcgtg ggtaatgcgc cacaaaacaa agccttcctg
    18301 caggaattac aagaagccag cgatcgcggt attgtggtgg tcaacctgac acaatgtatg
    18361 tccggtaaag tgaacatggg tggttatgcc accggtaacg ccctcgccca tgccggcgtt
    18421 attggcggtg cagatatgac tgtagaagcc acgctaacca aactgcatta cctgcttagc
    18481 caggaacttg atactgaaac cattcgcaag gccatgagcc aaaacctgcg tggcgaactg
    18541 acgccggatg attaaggaga ctgcaatgcc ccctcgcgcc ctgttactgg tcgatttaca
    18601 aaatgatttc tgtgctggtg gcgcgctcgc cgtgccggaa ggtgacagta cggtggatgt
    18661 cgctaaccgc ctgattgact ggtgccagtc gcgcggtgaa gcggttatcg ccagtcagga
    18721 ctggcacccg gcgaatcacg gcagttttgc cagtcagcac ggtgtagagc cttatacgcc
    18781 aggccaactc gacggtttgc cacaaacctt ctggccagat cactgtgtgc agaacagtga
    18841 aggcgcacaa ttacatccgt tactgaacca aaaagcgatc gcagcggtgt tccataaagg
    18901 cgaaaatcct ttagttgaca gttacagtgc cttttttgat aacggccgtc ggcagaaaac
    18961 cacgctcgat gactggttac gcgatcatga aatcgatgaa ttgatcatta tgggtctggc
    19021 tactgactat tgcgtgaagt ttaccgtgct agacgcgtta cagttaggtt ataaggttaa
    19081 tgtgattacc gatggttgtc gtggcgtgaa tatccagcca caggacagtg cgcacgcgtt
    19141 tatggagatg tcagcagctg gggcaacgct atatacgctg gcagattggg aagagacgca
    19201 ggagtaatat tagttggatt catctctggc ctacatttcc tcaatgacat tgtaggccag
    19261 gtaatacttg ttagtgttgc atttaaccta ctcgcagcta aaacacgaca tccgaacaag
    19321 tctatttgcc ctcccttaat aacaggctta aatactagac aactcattca cttaaacatg
    19381 gcaataggct ttgctgagat accaaaatct tcttttaact gttgtttgct cttcatgacc
    19441 atctgaccat ttgtgtcaat tgtcatatgc tgtgcttcgg tattattgcg ggcttgccac
    19501 agcatcacca gttgcaggca gttctctttt tgttcctcgg tcaacgcaac gccatcaggc
    19561 catttcccca gttcaacggc ggtcgacaaa cgctggtata cctcaggcgt catgctgttg
    19621 ataatgtcat caagattcat aatttttccg ctcctatgga ataagttgct gaatcgtttt
    19681 ttcaaccgtt gatttcttcg ccgttttcgc catcggtaaa gcgtaaagag gcagagttaa
    19741 cacaataacg ttcgcccgtt ggctgcggcc cgtcggggaa gacatgcccc agatgggcat
    19801 cacagttacc gcaacgaatt tctatgcgct gcattccatg tgacaagtct ttgatataac
    19861 gaatggattc ttcactcacc ggttcgtaga aactgggcca gccacagccg gaatcatact
    19921 tggtttggga atgaaatagc ggggcatcgc agatcaaaca gtgatatacg ccgtcacgct
    19981 tgttatgcag taaacgaccc gtaaatggcg gttctgtccc atgattctgc gtcacgtaaa
    20041 actgcatctc ggacaaattt tttttcagtt cttctgccga aggtttatta gccatttgct
    20101 cacatctcac tttaatcgtg ctcacattac gcgactgatt ctaacaaaac attaacacca
    20161 actggcaaaa ttttgtccta aacttgatct cgacgaaatg gctgcaccta aatcgtgatg
    20221 aaaatcacat ttttatcgta attgcccttt aaaattcggg acgccgaccc catgtggtct
    20281 caagcccaaa ggaagagtga ggcgagtcag tcgcgtaatg cttaggcaca ggattgattt
    20341 gtcgcaatga ttgacacgat tccgcttgac gctgcgtaag gtttttgtaa ttttacaggc
    20401 aaccttttat tcactaacaa atagctggtg gaatatatga ctatcaaagt aggtatcaac
    20461 ggttttggcc gtatcggtcg cattgttttc cgtgctgctc agaaacgttc tgacatcgag
    20521 atcgttgcaa tcaacgacct gttagacgct gattacatgg catacatgct gaaatatgac
    20581 tccactcacg gccgtttcga cggtaccgtt gaagtgaaag acggtcatct gatcgttaac
    20641 ggtaaaaaaa tccgtgttac cgctgaacgt gatccggcta acctgaaatg ggacgaagtt
    20701 ggtgttgacg ttgtcgctga agcaactggt ctgttcctga ctgacgaaac tgctcgtaaa
    20761 cacatcaccg ctggcgcgaa aaaagtggtt atgactggtc cgtctaaaga caacactccg
    20821 atgttcgtta aaggcgctaa cttcgacaaa tatgctggcc aggacatcgt ttccaacgct
    20881 tcctgcacca ccaactgcct ggctccgctg gctaaagtta tcaacgataa cttcggcatc
    20941 atcgaaggtc tgatgaccac cgttcacgct actaccgcta ctcagaaaac cgttgatggc
    21001 ccgtctcaca aagactggcg cggcggccgc ggtgcttccc agaacatcat cccgtcctct
    21061 accggtgctg ctaaagctgt aggtaaagta ctgccagaac tgaatggcaa actgactggt
    21121 atggcgttcc gcgttccgac cccgaacgta tccgtagttg acctgaccgt tcgtctggaa
    21181 aaagctgcaa cttacgagca gatcaaagct gccgttaaag ctgctgctga aggcgaaatg
    21241 aaaggcgttc tgggctacac cgaagatgac gtagtatcta ccgatttcaa cggcgaagtt
    21301 tgcacttccg tgttcgatgc taaagctggt atcgctctga acgacaactt cgtgaaactg
    21361 gtatcctggt acgacaacga aaccggttac tccaacaaag ttctggacct gatcgctcac
    21421 atctccaaat aagttgagat gacactgtga tctaaaaaga gcgacttcgg tcgctctttt
    21481 ttttacctga taaaatgaag ttaaaggact gcgtcatgat taagaaaatt tttgcccttc
    21541 cggtcatcga acaaatctcc cctgtcctct cccgtcgtaa actggatgaa ctggacctca
    21601 ttgtggtcga tcatccccag gtaaaagcct cttttgcatt acagggcgca caccttctct
    21661 catggaaacc tgcgggtgaa gaagaagttc tgtggttgag caataacaca ccgttcaaaa
    21721 atggcgtcgc tattcgcggt ggcgtaccgg tttgctggcc gtggtttggt ccggctgcac
    21781 aacaaggtct gcctgcgcac ggttttgccc gcaacctgcc gtggacgctg aaatcacatc
    21841 gtgaagatgc tgatggcgta gcgctgactt ttgaattgac acaaagcgaa gagacgaaaa
    21901 aattctggcc gcacgacttt acgctgttag cgcatttcca cgtgggtaaa acctgtgaaa
    21961 tcgatcttga agcacacggc gaatttgaaa ctacctctgc tctgcatacc tactttaacg
    22021 tgggtgatat cactaaggta agcgtcagtg gactgggcga tcgcttcatt gataaagtga
    22081 atgacgcgaa agaagatgta ctgaccgacg gtattcagac cttccctgac cgtaccgatc
    22141 gcgtgtatct gaatccgcaa gattgcagcg tgattaatga cgaagcgctg aatcgtatta
    22201 tcgccgtagg ccaccagcat catctgaacg ttgtaggctg gaacccagga ccggcgcttt
    22261 cagttagcat gggcgatatg ccggatgatg gctacaagac ctttgtttgt gtagaaacag
    22321 cttacgcctc agaaacgcaa aaagtcacca aagagaaacc tgcacatctg gcgcaatcca
    22381 ttcgcgttgc gaaacgttaa tttgcgttag tgttgtgtgc cgggtgcgat gcatccggca
    22441 cgtatcaaca tcacaccata tccagcgcag tttttccttt tggtgccgga tatgccttat
    22501 ccagcatagc taattccgct gatgaaagtt cgacctcaag cacagccgca ttttgttgga
    22561 catgggcaat cgtggccgct tttggaatcg ccatcacacc ctgatgactg atcacccacg
    22621 ccaacaatac ttgtgccgcg ctgatattgt gagcatgtgc aatttcgttg actaccgcgt
    22681 tttttaacag cccattgcgc aaccgcccgg cctgggctaa cggactgtaa gccatcaccg
    22741 gcatctgctg tttctggcac cagggaagta gatcgtactc aatccctcgt gaaccgagat
    22801 ggtaaagcac ctgattagtg gcacactgat tcccccccgg cagctgccag agttcctgca
    22861 tatcggcata atcaaggtta gaaacgcccc agcggcggat ttttccctgg gcgatcaatt
    22921 tttccatcgc tgcgacagtc tcttcaaaag cgaaactgcc agaccagtgt aataagtaga
    22981 gatcgagata atcagtattg agacggcgta aactggcttc gcatgcattt atcgcttttt
    23041 gcccgccagc attccacgga tagactttag agacgagaaa gacgttatca cgcagaccga
    23101 ttaatgcttc cccaaccact ttttcggcac cgccatcggc atacatttcg gcggtatcaa
    23161 tgagggttaa accgagttca atgcccgcgc gtagtgcagc aacttctgtt ttacgctgac
    23221 tggcatcttc gcccatatac catgttccct gccctatggc tggcagtgag acatcgccac
    23281 taaattgaat cattttttgt tgcattgttt cctcccaggt attgcaccac cgtaatgcaa
    23341 aacagggcat gacgccctgt ttttatgcac aaaatgccct ggaaagatgc attatcagaa
    23401 tttgtaggtg atcccggtag aaatcaggcc agtccaggat ttatccacca ttgggctgtc
    23461 agtaacttca tcagacagac gggtgtagcg cgcggtaccg taaacactcc agtcgccgag
    23521 gaagttgtag ctggcgctca gctccaggta agggctccag ctgtcgttcg ggttatagcc
    23581 acgcagaccg ctgcgagcgg actctttgcg cgatacgcca taatagtatt cgttctggtt
    23641 ttcgctgttc cactgcacac caatacccgg agtcacggtc aggccgccgt tggtgtaacg
    23701 atacaaccag gccatatccc agacaatgcc gttgctgtta tccagggtat cgccagccag
    23761 ggtggtacgc aggtaaccgt actgggtaaa gtgagcataa gacagaccag ccatcatggt
    23821 gctcttacgg tcatccaggt gacgcatttg gtgatcgcca ctgtctttag ctttgaagta
    23881 aagcggcgac cagtaagcgg taattgaaag tttgtccgtt gcgtcattcc acaggtagta
    23941 accaccacct aagccacgga accagaagtt atcgccttca tagttgatta ccggtactgg
    24001 gtaaacatcg gtatcgtaat ctttatatgg gtgttcaacg acacctacgc ctgcgcccag
    24061 ggaaaattta ccttcagcgt gcgctacgcc tgcagacgtt gcgataagca ctccaagtgc
    24121 cagaagtttg agtttggtca caattaatca ttccttaaac aaatgtttag cgggcgacaa
    24181 agtttacccg tcaatacatc gaaacccaac ctttttacgt tttcattttt taaagtaact
    24241 gtttaatttt cctgacgcag atgacaccac gcttacagcc aaatgaattt agcgttacct
    24301 gcggccctat tgtttacatt aattatgcaa aatttatgga tgagttgttg atatgccatt
    24361 gaaattaaga aagccgtgca ggcaagtttt ccatttgcca tctacgctta attttgaagg
    24421 tgtatcaccg ggcacgttgt tctcatcgtc gataaaatgg catgagagtt gctgtgtttt
    24481 agcaagagac gtcgttcagt ttacctcttc cgggagcctc tactattcat atgaacggct
    24541 cttaacctgt gctaaaaaac gaaaggacgg cataccatga atatattcga tcactatcgc
    24601 cagcgatatg aagctgccaa ggacgaagag ttcacactgc aggagtttct taccacttgt
    24661 cggcaagatc gcagtgctta tgccaacgcg gctgagcggc tattgatggc tattggtgag
    24721 cctgtcatgg tcgatacagc ccaggaaccc agactttctc gactcttttc taaccgggtc
    24781 attgcacgtt atccggcgtt tgaagagttt tacggcatgg aagacgcgat tgaacagatt
    24841 gtctcttatc tgaaacacgc ggctcagggg ctggaagaga agaaacaaat cctgtatctg
    24901 ctggggcctg tgggtggggg taaatcatcg cttgctgagc gactgaaatc attaatgcag
    24961 ctcgtgccga tctatgtatt aagcgctaac ggtgagcgga gcccggtcaa cgatcatccg
    25021 ttctgtcttt tcaatccgca agaagatgcg cagattctgg aaaaagagta tggcattcct
    25081 cgacgctatc ttggcaccat catgtcgccg tgggcagcaa aacgcctgca tgaatttggt
    25141 ggcgatatta ctaagttccg ggtggtaaag gtctggccgt caattctgca acaaattgct
    25201 atcgccaaaa cggaacctgg cgatgagaac aaccaggaca tctcagcgct ggtagggaaa
    25261 gtcgatatcc gtaaactcga acactacgcg cagaacgatc cagacgccta cggatattct
    25321 ggtgcactgt gccgcgccaa ccaggggatc atggagtttg tcgagatgtt taaagcaccg
    25381 ataaaagtgc tgcatccctt gttaaccgcc acccaggaag gtaactacaa cgggacggaa
    25441 ggtatctccg ccctgccgtt caacgggatt attctcgccc actcgaacga atccgaatgg
    25501 gtcactttcc gtaataacaa aaacaacgaa gccttcctcg accgtgttta catcgtgaag
    25561 gtgccgtatt gcttgcgcat ttccgaagag atcaaaatct acgagaaatt gcttaatcac
    25621 agtgaattga ctcacgcccc ctgcgcccct ggcacgctgg aaacgctgtc acgtttttct
    25681 attctttcgc gtctgaaaga gccagaaaac tccagcattt attcaaagat gcgggtttat
    25741 gatggtgaaa gtttgaaaga cactgatccc aaagccaagt cgtatcagga atatcgtgac
    25801 tacgccggtg tcgatgaagg gatgaacggt ctgtcgacgc gttttgcgtt taagatcctc
    25861 tcccgcgtgt tcaacttcga tcatgtagaa gtggcggcca acccggttca tctgttctac
    25921 gtcctggaac agcagatcga gcgcgagcag ttcccgcaag aacaggcaga acgctatctg
    25981 gagttcctga aaggttatct gatcccgaaa tatgccgagt ttatcggcaa agagatccag
    26041 acggcctacc ttgaatccta ttccgaatat gggcaaaaca ttttcgaccg ttatgttacc
    26101 tacgcggatt tctggattca ggatcaggag tatcgcgatc ctgataccgg gcagttgttt
    26161 gaccgcgagt ctcttaacgc cgagctggag aaaatcgaga aaccggcggg gatcagtaat
    26221 ccaaaagatt tccgcaacga gattgttaac ttcgtactgc gcgccagagc gaataacagc
    26281 ggacgcaatc cgaactggac cagctatgaa aaactgcgca cggttattga gaagaaaatg
    26341 ttctccaata ccgaggagct gttgccggtt atttcgttta acgccaaaac gtcaaccgac
    26401 gagcagaaga aacatgacga ctttgtcgac cgtatgatgg aaaaaggcta cacccgtaaa
    26461 caggtgcgtt tactgtgcga atggtatttg cgcgtacgta aatcgtctta acaaccctgg
    26521 cccggtacac atgctatcgg gcctacaacg acagcgaacc gtgggccaga gaagcggcaa
    26581 cacaggcgta gcataaagtt ggcaaatgta gtacgggggg catatgacct ggtttattga
    26641 ccggcgtctg aacggcaaaa acaaaagcat ggtgaatcgc cagcgttttt tacgccgtta
    26701 taaagcgcaa attaaacagt cgatctccga ggccattaat aagcgttcgg tgactgacgt
    26761 cgacagcggc gagtccgtat ccattcccac ggaagatatt agcgaaccga tgtttcatca
    26821 ggggcgtggc ggtctgcgcc accgcgtgca tccgggcaat gaccatttcg tccagaacga
    26881 ccgaattgaa cgtccccagg gtggcggcgg aggttccggc agcggtcagg gccaggccag
    26941 ccaggatggt gaaggtcagg atgaattcgt ctttcagatt tcgaaagatg agtatcttga
    27001 tctgctcttt gaagatttgg ctttaccgaa tctgaaacaa aaccaacaac gccagctgac
    27061 cgaatataaa acgcatcggg cgggttatac cgctaacggc gttccggcca atatcagcgt
    27121 tgtgcgttca ttgcagaact cactggcgcg gcgcacagcc atgacggcgg gcaagcggcg
    27181 ggaacttcat gcgctggaag agaatttggc catcatcagc aacagtgaac ctgcgcaact
    27241 gctggaagag gaacgtctgc gcaaagaaat tgcagaatta cgtgccaaaa tcgaacgcgt
    27301 ccctttcatt gacaccttcg atttacgtta caagaactac gagaagcggc ccgatccctc
    27361 cagccaggca gtgatgtttt gcctgatgga cgtttccggt tcaatggatc agtccactaa
    27421 agatatggct aaacgttttt atattctgct gtatctgttc ctcagcagaa catataagaa
    27481 cgtggaagtc gtatacatcc gccatcatac ccaggcgaaa gaagtcgatg aacatgagtt
    27541 tttctactcg caggaaacag gcggcaccat tgtttccagc gccctgaaac tgatggatga
    27601 ggtagtgaaa gagcgttata acccggcaca gtggaatatt tacgctgcac aagcatcgga
    27661 cggcgataac tgggccgatg actctccgct ttgccatgaa atcctggcga aaaaattatt
    27721 acccgtcgtt cgttattaca gctatatcga aattacccgt cgtgcacatc agacattgtg
    27781 gcgagaatat gagcatctgc aatctacttt cgacaacttt gcgatgcagc atatccgcga
    27841 ccaggatgat atttatccgg tgttccgtga actgtttcat aaacaaaatg caacagctaa
    27901 agactaaaac tgtcagccgg gtcattatcg cctggctgat ttttagctca ctgtaaatta
    27961 tctcatttat tacatacatt agcttacaat cgctttaaat atgacagcat aacctttaca
    28021 caatttagtt ccagaaaaca atcattcgga aaaatgattc gggcaacacg tatttccctg
    28081 gggttattct ttaattattt tttatcgtta acgaaaattg atcctggtca aaactatata
    28141 tctctgccat caataaaatc cagcactcac attgctctcc tttttatggc ttctatgggt
    28201 acacaaaaat taaaagctca aggctttttt attttcagct tattgctgac gttaatttta
    28261 ttttgcatta ctaccttata taacgaaaac acgaatgtaa aactcatccc acagatgaat
    28321 tacctgatgg ttgttgtggc tttgtttttc cttaacgccg tcatttttct tttcatgtta
    28381 atgaagtatt tcactaacaa acaaatttta ccaacactca ttttaagcct tgcattttta
    28441 agtggcctta tctatttagt tgaaaccatt gtaattatcc ataaaccaat caacggcagt
    28501 acactgatcc agacaaagtc gaatgatgtt tctattttct atattttccg ccaactcagt
    28561 ttcatttgtt taacctcgct ggcgctcttt tgttatggga aagacaacat ccttgacaac
    28621 aataagaaaa aaactggcat tctgttactg gcgctgattc cttttttagt ttttcccctt
    28681 ctggcacaca atctgagcag ttataacgct gactattctt tgtatgtcgt cgattactgt
    28741 ccggacaacc atactgcgac ctggggaatc aactatacaa aaatattggt ttgtctgtgg
    28801 gcatttttac tgttctttat tatcatgcgt acacgattag ccagcgaact atggccgtta
    28861 atagcattat tatgtctggc atcgctatgc tgcaacttac ttctactaac tctggatgag
    28921 tataattaca ccatctggta tatcagtcgc gggattgaag tttccagtaa actgtttgtt
    28981 gtgtcttttc tgatttataa catttttcag gaactgcaac tctccagcaa actggcagtt
    29041 catgatgtac tgaccaatat ttataatcgg cgctactttt tcaacagcgt agagtcatta
    29101 ttgtcgcgac ctgttgttaa ggacttctgt gtcatgctgg ttgatattaa tcagttcaaa
    29161 cgcatcaatg cccaatgggg acatcgtgtg ggtgataaag tgctggtttc aattgtcgat
    29221 attatccagc aaagcatccg ccccgatgat attttagcgc gactggaggg tgaggtgttt
    29281 ggcttgctat ttaccgaact caatagtgcc caggcaaaaa tcattgcgga acgtatgcgt
    29341 aaaaatgtcg aactcctgac cggctttagt aacagatatg atgttcctga gcaaatgacc
    29401 atcagtattg gcacggtttt ttcaacgggt gacacacgta atatctcgct tgtcatgacg
    29461 gaagcagata aagccttacg cgaagcgaaa agcgaggggg gcaacaaagt gattattcat
    29521 catatttaag tgcaaaaata ttcagagcca tgctttttgc gtggcttttg catacaattt
    29581 attactatta cccctaaatt tcccctcccc agcggcgcag agatgagtat aattagcgcc
    29641 cctgtgccag gccgcaatcg aactttatct ggttttctcg tttcactaac cgaaggagtg
    29701 ccatttatca tgaaattgca ccatagaatg ctccggcatt ttatcgccgc aagtgtcatt
    29761 gtgctgacat cttccttcct tatttttgaa cttgtcgcca gcgacagagc aatgagtgcc
    29821 tatctgcgct atatcgtgca gagagcagac tcctcctttc tttatgataa gtatcagaat
    29881 cagagtattg ccgcgcatgt gatgcgcgct ctcgctgctg agcagtcgga agtgtcgcca
    29941 gaacagcggc gcgccatctg cgaggctttt gagtctgcca ataataccca tggcttaaac
    30001 ctgactgccc ataaataccc tggcttacgc ggcacactac aaaccgcatc cactgactgt
    30061 gacacaattg tggaagctgc tgcactatta cccgcttttg atcaggcagt ggaaggcaac
    30121 cgccaccagg atgattacgg ttcaggtctt gggatggccg aagagaaatt tcactattat
    30181 ctcgatctca atgaccgcta tgtctatttt tatgagccgg ttaatgttga gtactttgcg
    30241 atgaataact ggtccttcct gcagtcagga agtattggca tcgatcgcaa agatattgaa
    30301 aaggtattta ccgggcgtac cgtattatcg agcatttacc aggatcagcg tactaaacag
    30361 aacgtgatga gtttactgac gccggtatat gtcgcagggc agctaaaagg gattgtgctg
    30421 ctggatatta acaaaaacaa tctgcggaat atcttttaca ctcatgaccg ccctctcctc
    30481 tggcgttttc tcaatgtcac gctaactgat acagattcgg ggcgcgacat tatcatcaac
    30541 cagagcgaag ataatctgtt ccagtatgtc agttacgtcc atgacttacc gggcggcatt
    30601 cgtgtctcgt tatccattga tattctttac tttatcacgt cttcgtggaa aagcgttctg
    30661 ttctggattt tgacggcgtt aattttgctg aatatggtgc ggatgcactt ccgtttatac
    30721 caaaacgtgt cgcgagaaaa tattagtgat gcgatgactg gactgtataa tcgcaaaatt
    30781 ttaacccctg aactggagca gcggttgcag aagctggtgc aatccggttc ttcggtgatg
    30841 tttattgcaa ttgacatgga caagttaaag caaataaatg acaccctcgg tcatcaggag
    30901 ggggatttag cgattacgtt attagctcag gcgattaaac aatcgattcg taaaagtgat
    30961 tatgccatcc gactcggtgg cgatgaattc tgcatcattc ttgtcgattc gacgccgcaa
    31021 attgcagcac aactgcctga acgtatcgaa aaacgtctgc aacatatcgc gccgcagaaa
    31081 gagatcggct tctcttccgg tatttacgcg atgaaagaaa acgatacgtt acatgatgcg
    31141 tataaagctt ccgatgagcg tttatatgtc aataagcaga acaaaaacag ccgttcatga
    31201 taaccttctg tggttgtttg cttgtaatct caggagcatg aaatgactga aatggctaaa
    31261 ggaagcgtga cgcatcagcg gttaatcgct ttattatcac aagaaggtgc tgacttccgc
    31321 gtcgttaccc atgaagcggt aggtaaatgt gaagcggtgt cagaaattcg tggcaccgct
    31381 ttaggccagg gggctaaggc actggtatgt aaagtcaaag gcaatggtgt aaatcaacat
    31441 gttctggcga tcctcgcggc cgatcaacag gccgatctga gccaactcgc cagccatata
    31501 ggcggattac gcgcttcgtt ggccagcccg gcggaagtcg atgaactcac aggctgtgtc
    31561 ttcggcgcga tccccccttt cagcttccat ccaaaactca aactggttgc cgatccccta
    31621 ctcttcgaac gatttgatga aatcgccttc aatgcaggca tgctggataa atccgttatt
    31681 ttgaaaaccg cggattattt gcgcattgcg caaccagaac tggtcaattt ccgccgcact
    31741 gcgtaactag cctgtccgtt cgataagaag aacggacaaa accagcacaa cagcaatggc
    31801 aaaaaacgat gacgtgataa tcagtgtctc gacgaacatt tgatcgttca tagtatgccc
    31861 ccgggagata gttgttttca cgttattgtt agcgtgcgca aatggcagtt tgatgacagt
    31921 tcgcctgatt ttttatgcaa aaaacgtaaa gattttctac ttccttcctg cagcaagcgt
    31981 aaagtaagca ggcttattat tttttggcaa ggaaaccacg atgtttgatg tcactttgct
    32041 gatcctgctc gggttagctg cgctgggctt tatcagtcat aacaccactg tcgccgtttc
    32101 aattctggtg ttaatcattg tccgcgtcac accgttaagc acctttttcc cgtggattga
    32161 aaaacagggg ctaagtatcg ggataatcat tctgacgatt ggcgttatgg ctcccatcgc
    32221 cagcgggacg ctaccgccct caaccctgat tcactcgttt ctgaactgga aatcgctggt
    32281 ggcaattgca gtcggtgtta ttgtctcctg gctggggggg cgcggcgtga cgttaatggg
    32341 cagccagccg caactggtcg ccgggctgct ggtgggcact gttttaggcg tggcgctgtt
    32401 tcgcggcgta ccggttggcc cgcttattgc cgcgggtctg gtttcgctga ttgtggggaa
    32461 acagtagtta atcccgcaat atatcggcct ggcgtttgcc ccaggccttt cttaaacata
    32521 gtaataaagg ccgttgttga atcatacccc agcgtatgag caactttctg tacagtatcg
    32581 cctttcacta atccctgaag cgccataatc agctgtaact gctgacgcca ctggcgaaaa
    32641 ctcaaccccg tctctttcac tatcagccgg gcaagattgc gttcactcat cgcaaaaaag
    32701 ccagcccatt gccccaatga gccccactcg acgggctctt tcgccatcat ctccaccatt
    32761 gtgcggattt taggatgaga agaaacgggc aggtgcagct gttgttgtgg ctgctgaggg
    32821 agttcatcaa acaggacctg aattaatctg cgggtcatcg gctcagcacg ctgagtcgtc
    32881 gtcctgtttg ccagggttaa aattaattcc cggcataagg gcgatatttt caatgtgcaa
    32941 caggttgtcg gcattgtcac tgcgctgggt tcgataaaca gaaaacagag ttcagcgtta
    33001 gctgtcactt gattgctatg ttccacccct cccggtatcc aaaccgcata ttgtggcggc
    33061 accatccaca aagcattttc caccgtacag gtaattgcac catgtagtgc cagtatcaac
    33121 tgtcctttgc ggtgctgatg tacaggactg gttaactcat ccgttccggc gtgaatacaa
    33181 aatgcgaccg cggcttcatg gtgtcggtcg ggttcatagc cattgagatt caacctgtgc
    33241 atcattttgt ccgaatttag cgatattttg tcattttagc ttgattcaac ataacaataa
    33301 aaacggtaag gtacagcctc gtttgtaaca atgagaagca tatgacctgt tcaacttcat
    33361 taagcggcaa aaacaggatt gtccttatcg ctggcattct gatgattgcc acaacattac
    33421 gcgtcacctt taccggcgca gcaccgttac tggatacgat tcgttccgct tactcgctaa
    33481 cgacggcgca aaccggctta ttgaccaccc tgccattatt agcctttgcg ctaatctcac
    33541 cgctggccgc cccggtggcg cgacgttttg gtatggaacg tagcctgttt gccgcgttac
    33601 ttttgatctg tgcaggtatc gcaattcgct ctctcccttc gccttactta ttatttggcg
    33661 gtacagcgat cattggcggt gggattgcat taggcaatgt cttactgcca ggattaatta
    33721 aacgcgattt ccctcattcc gtcgccagac ttaccggcgc atattccctg acaatgggag
    33781 ctgcagcggc actgggatcg gcgatggtag tgccgctggc tttgaacggt tttggctggc
    33841 gaggcgcgtt gctcatgctg atgtgttttc ctctgctggc tcttttttta tggctgccac
    33901 agtggcgaag ccaacaacat gcaaatttga gcacctcacg cgccttacat actcggggta
    33961 tctggcgttc gccgcttgcc tggcaggtaa cattgtttct tgggatcaac tcactcgtct
    34021 attacgtgat tattggctgg cttccggcga tcctcatcag tcacggctat agcgaagcac
    34081 aggcgggttc actgcatggt ttgctgcaac tagccacagc agctcccggt ttgctgatcc
    34141 cacttttctt gcatcatgtg aaagatcagc gtggtattgc agcgttcgtt gccttgatgt
    34201 gcgcagtggg cgcggttggg ctctgcttta tgccagcgca cgcgatcacc tggactctgc
    34261 ttttcggctt tggttccggc gcaacaatga tactggggtt gacgttcatt ggtctgcggg
    34321 caagttctgc gcatcaggcg gcggcactct cggggatggc acaatccgtc gggtatttgc
    34381 tggcagcctg tgggccgccg ctgatgggta aaatacacga tgctaacggt gactggtccg
    34441 taccactgat gggtgttgca atactttcac tactgatggc gattttcgga ctttgcgccg
    34501 ggagagacaa agaaattcgc taatatccgg tgctatagtg acgtgacaaa tcatgcgtga
    34561 aagggagaac aaacacgatg aatattcagt gcaaacgcgt ttatgatccg gctgaacaga
    34621 gcgatggtta tcgcgtactg gtcgaccgcc tctggccgcg cggtatcaaa aaaaccgatt
    34681 tagcccttga tgagtgggat aaagaaatca cgccgtcaac ggaactgcgc aaagcctttc
    34741 acggcgaagt cgtcgatttt gcaacctttc gcgagcaata tcttgcagaa ctggcgcaac
    34801 acgagcaaga aggaaagcgg ctggcgaaca tcgccaaaaa acagccgctg accctgctct
    34861 actcagcaaa aaacaccacg cagaaccatg cgctggtgct ggcagactgg ctacgtagct
    34921 tgtgatttta gtacgccatc cggcggttat ttttcaccag ccggatggtc ccgtcgccac
    34981 aatgcccatt catcaatcgt ttcaccgccc ggtaatttgc aattgttgct gaccccttgc
    35041 gctgtctgca caggaatgag cgtcccgccc ttctgctggc aatagaccga cgccggattt
    35101 gccataccaa tctgcggcgg tttaggtgct tctggctgag aaggggttga acaaccagcc
    35161 aggaccagca agcaaggcag aacaaaactg ataattttca tttattgatc tcacatattt
    35221 atccagactt agagtatcgc ggtatcgttt tgttttgcag cactattttt attacattca
    35281 ctcaaatcat attacgcctt gtttcatctt tgttgatgat gttttatcat gcctgcaaag
    35341 attaaataat cagcatttac ccgccgtatc ctggagttgt ttcgtgtcag atcagattat
    35401 cgcccgcgtc tcgcaatccc ttgccaaaga acagtcactg gaaagcctgg tccgacagct
    35461 tctggagatg ctggaaatgg tcactgatat ggaatcaacc tacctgacca aagtggatgt
    35521 cgaagcgcgc ctgcagcata taatgtttgc ccgtaacagc cagaaaatgc acatcccgga
    35581 gaattttacc gtctcgtggg attactcgtt atgcaaacgc gccattgatg aaaactgctt
    35641 tttcagcgat gaagtccctg accgttgggg cgactgtatt gcggcacgca atcttggcat
    35701 caccacattt ctgagcacgc caattcactt accggatgga tcattctatg gcacgctttg
    35761 cgccgccagt agtgagaagc gccagtggag tgaacgcgcg gaacaggttt tacagttatt
    35821 cgccggactg attgcacaat atattcaaaa agaggcactg gttgaacagc tgcgcgaagc
    35881 caatgctgcg ctgattgcgc aatcgtatac cgactcgtta accgggctac cgaatcggcg
    35941 ggcgattttt gaaaatctga cgacgctgtt ttctctcgcc cgacatctta accataagat
    36001 aatgatcgcg tttatcgatc tggataactt caaattaatc aacgatcgtt ttggtcataa
    36061 tagtggtgat ttgttcctca ttcaggttgg cgagcgcctt aatacgctcc agcaaaatgg
    36121 cgaagttatt ggtcgtctcg gcggtgatga gtttttggtt gtttcactga acaacgagaa
    36181 tgcggatatt tcgtctctgc gagaacgtat tcaacagcaa atacgtggag aatatcactt
    36241 aggtgacgtt gatttgtatt atcccggtgc cagtcttggc atagtagaag tcgatcctga
    36301 aacgaccgat gcagacagtg ccctgcatgc tgccgatatc gcgatgtatc aggagaaaaa
    36361 acacaaacag aaaacacctt ttgtcgcgca tccagcgcta cattcctgag gcgtattcac
    36421 atccttttga ttggtgataa catgcgaatc ggtattattt ttccggttgt aatctttatt
    36481 acagcggtcg tatttttagc atggtttttt attggcggct atgctgcccc gggagcataa
    36541 agatgaaaaa aacaacgatt attatgatgg gtgtggcgat tattgtcgta ctcggcactg
    36601 agctgggatg gtggtaacgt cacatctaga aaatagcaaa ggctgcctgt gtgcagcctt
    36661 tgtgcaattt acgcgttaac ttttaatctt tctgtagata aacagcacga caatcgcgcc
    36721 aataacggca accacgaagc tgccaaaatt gaagccatcg actttaccaa agccaaacag
    36781 cgtgctgatc catccgccga ctacggcacc gactatcccc agcaggatag tcataaagaa
    36841 tccaccacca tctttacctg gcatgatcca cttcgccaga ataccggcaa taagcccaaa
    36901 aataatccat gacagaatgc ccattgtttc ctcacttatc tgttttgcat tagcgggtta
    36961 gtcgctgata aaaagcatag cacaacatcg tgagggcaag atttgtgacg agcatcacgg
    37021 aggatttttt tgcgatggcg cagaaattgc gccatcaacg atcagtgata attaccaacc
    37081 acaaacatca tgttcgtttt ccgtgtcata agaacgcacg gtattcacca gatcttttat
    37141 cacttcagcc gccacttctg gcaccagcaa agtcatcggc gtctctgttt cataatcgac
    37201 agaaacgcca ttgctgttat tggtgacggt cacggtatac gttgctttgc ccatgattca
    37261 tttcccgtta tgaatgactt tccgttgttg cgcaccttcc atcaggactt caggagcaac
    37321 gaagaagtca atgttgaaat aagtgtcgtc agtcatggct tcaatgttgt gccacttttc
    37381 tggagggaac acagcaaact gccccgcttc gataaggatc acttgatcag gctctgtact
    37441 gtgttcatca gcgtagccga gatatttgac cgccccatgc ataacggaaa ggcgtgggta
    37501 aacccccggg cgcgttcctt tatcaagatg acgttcaaat attccggcag gcgcagtttg
    37561 tttattccag aaaggcgttg agcgcgtatg aatataattc tgtgggattt gcagcatcct
    37621 tttccctcct tcggtgaatg cgctgaaaac ggtttatttc agccgtttca gggtacgcct
    37681 gataatttgc attttaaata ccatttattg gttactattt agcaccgtat cagagaagaa
    37741 tcagggagga ttacaggtgg gaagcccgcg acaacgcggg ctgaatgcat caggactgga
    37801 gcgtcgccag tcgggcggcg aaacccacaa acatcaaacc aatcagtgag ttgcccactt
    37861 tagccagttt ctttttggta cgtatgtact gcgtgacaaa agcaccagaa ataatcagga
    37921 agctcaaata gcagaaactc accagttcca gcgtcgtcgc cagaataaag aatgaaattc
    37981 ccgtatgtgg ggcattaaca tcgataaact gtacgaaaaa cgacacatag aacaaaatgg
    38041 ctttcggatt agtcaggctc aaaattaacg cgcgtttaaa aatggcaccg tattggggct
    38101 catcggattt ggcctcgtta tttttaccct tcagggtcgc gtagagaatt ttactcccca
    38161 gatagagcaa ataaaatgca ccaagataac gtacaatgtt gaataatatc ggggtggtct
    38221 taattaatgt cgccactcca gcccatgcca gaaacatcaa taccgcatcg ccaataaata
    38281 caccacaggc cgcaagataa ccgcctttca taccgctact gacgctattt ttgagtacaa
    38341 acagggtatt tggccctggc accaacacaa taaaaatggc cccaaccaga taggtccagt
    38401 aattcagaac cccgtattca gcgaacacat taacctcttt aattatcttt cgatcatgcg
    38461 cgattaaagg tgaatatgct aaccaatctg tagctgctta gaaaggagaa aatcaggttt
    38521 taacccgata tcaacgcgat aattgaatca ttaatcaggc atgctccagt gaaaaattcg
    38581 ggtagtgctc ggcaaaatac tggcgtaaaa attctaccgt tatccgcact ttcgctgacg
    38641 tcgccagcct tgaaacataa acggcccaga cgttcgctgg ctggtaatat tccggtagca
    38701 cttgcactaa atgaccactg gcaatgtttt cgctaacatc ccaccaggag cgcagagcaa
    38761 tcccctgccc atcgagacac cactgatgga caatttctcc atgattagac gacagtgggc
    38821 cggtaacttt aatcgcatgg tggccttctt tattgcgtaa ttgccagaca ccaaaaggat
    38881 gatcgcgctc tttaatgacc aggcacggca atgcagaaag atcggttaaa tgtttcggcg
    38941 cgccatgttg cgcaataaat tccggtgacg cgcagagaat gcgataattg gtcgccagtt
    39001 tgcgggcgat taaattaggg gcgatatcat cgccaatgcg aatatcgaga tcgacacctt
    39061 cattgaccaa atcgaccaac cggtcttcca catcaaaacg taattccagt tgcggatacg
    39121 ccttcgccag cgctgataac gccggagcca ccacctgtcg tccaaaacca aagctgctga
    39181 taatacgcag catcccctgt ggcacctgac gcacgtcaga aagttcgtcc atcatctgac
    39241 cgacatcctg caaaatccgc tgcgcccatt cataaatccg ctctccttct tcggtaatgg
    39301 tgacgcggcg ggtggtgcgg tgtaacaaca ccacgtttag cgtttgctcc agcaaggcga
    39361 tgcgcttgct gacgaacgcc ggagaaacgc ccaattcttc tgccacggcg gcaaaaccag
    39421 cccggcgagc caccagcata aagacgcgca aatcgttcag cagcggtaaa ttattcatga
    39481 ttcgtgtttt atgtttcacc agttacaggg attaattcct tttcagtcaa ttataggatg
    39541 gtgatgttgt caattttgat ggtcaggaag tgagaaccca atgatgaaaa cgatgcgtat
    39601 tgctgcgatc ccgggagacg ggattggcaa agaagtcctt cctgaaggga ttcgcgtgtt
    39661 acaggctgcc gctgagcgct ggggcttcgc cttgagtttt gagcaaatgg agtgggcgag
    39721 ctgcgagtat tacagccatc acggtaaaat gatgccggac gactggcatg agcaacttag
    39781 ccgtttcgac gccatctatt ttggtgccgt cggctggccg gacaccgttc cggaccatat
    39841 ttcgttgtgg ggttcgctgc tgaaatttcg tcgtgaattc gaccagtacg tcaacctgcg
    39901 cccggttcgt ctctttcctg gcgttccctg cccgctggcg ggaaaacagc ctggcgacat
    39961 cgatttttac gtggtcaggg aaaacaccga aggcgaatat tcctcgctcg gcggcagagt
    40021 gaatgaaggt acagagcatg aagtcgtcat tcaggaatcg gtatttactc gtcgtggtgt
    40081 cgatcgtatt ttgcgctatg cctttgaact tgcgcaaagc cgcccacgta agacgctaac
    40141 ttctgccact aaatcgaacg gtttagccat cagcatgccg tactgggatg agcgagtgga
    40201 agcaatggct gagaattacc cggagatccg ctgggacaag cagcatattg acattctctg
    40261 cgcacgtttt gtgatgcaac cggaacgctt cgatgtggta gtggcgtcca atttgtttgg
    40321 cgatatcctt tccgatcttg gcccggcctg caccggcacc attggcattg ccccatccgc
    40381 caacctgaat ccggaacgca ctttcccgtc gctcttcgag ccagtccacg gttccgcgcc
    40441 ggatatctac gggaaaaaaa ttgctaaccc tatcgccaca atttgggccg gtgcaatgat
    40501 gctcgatttt ctcggcaatg gcgatgagcg tttccagcaa gcgcataacg gtattctggc
    40561 cgcgattgaa gaagtgattg ctcacgggcc gaaaacacct gatatgaaag gcaatgccac
    40621 cacgccacag gttgccgacg cgatttgcaa aattattttg cgttaaggtc aaaccagttt
    40681 atttgaaccg cgtcactgac gcggtttttt tattcgttct ttgcagtaaa taacctgcgt
    40741 catttcacct tttattgttt ccgtttcgtg ttttatggct ttccgtattc ttaattgttt
    40801 aatttatgta acatgcaaat aatcaatttg ttaattacga caaacaactc attaccctgc
    40861 aaataattac aactggcaaa agccagagac gggcttttat gcccaggaga tgactcatga
    40921 tgagcaatgt taagaaaaaa gatgtgccgt tgataagcat cagcctggtg gccattcttt
    40981 tcatcgcagc tacattaagc cttttcccac aacaatcggc cgacgcggcc aacgctatat
    41041 acacttttgt tacgcgtacg ttaggttccg ccgtacaggt attggttttg ctggcaatgg
    41101 gactggtgat ttatttagcc accagtaaat acggcaatat tcgtcttggc gaaggaaaac
    41161 cggaatacag cacgctctcc tggctgttta tgtttatttg tgccggtttg ggttcttcta
    41221 cgctttattg gggggttgct gaatgggcct attattatca aacgcctgga ttaaatatcg
    41281 caccgcgttc acaacaggca ctcgaattta gcgttcccta ctccttcttc cactggggca
    41341 tcagcgcctg ggcaacttat acgctggcct cattaatcat ggcttatcac tttcatgtgc
    41401 ggaaaaataa agggctgagc ctttccggca ttattgctgc tattaccggc gttcgcccgc
    41461 aaggcccgtg ggggaaactg gtcgatttga tgttcctgat cgccactgtc ggcgcactga
    41521 ccatttccct tgttgtcacc gcagcaacct ttacccgtgg gctttccgcg ctgaccggtt
    41581 tacccgataa ctttaccgta caggcatttg tgatcctgct ttccggcggc attttttgcc
    41641 taagctcgtg gattggtatc aacaacggtt tgcaacgtct aagcaaaatg gttggctggg
    41701 gcgcgttcct gctgccatta ctggtgctga ttgtcggtcc aaccgaattt attaccaaca
    41761 gcatcatcaa tgccatcggc ctgaccacgc aaaacttcct gcaaatgagc ttattcaccg
    41821 atccgcttgg cgatggttca ttcacccgca actggaccgt tttctactgg ctgtggtgga
    41881 tctcatacac gcctggcgta gcaatgtttg tcacccgcgt ttcccgcggt cgtaagatta
    41941 aagaagttat ctggggactg atcctcggca gcaccgtcgg ttgctggttc ttcttcggcg
    42001 taatggaaag ctatgccatt caccagttta tcaatggcgt aatcaacgtc ccacaggtgc
    42061 tggaaacact gggcggcgag acagctgtgc agcaagttct gatgtcgttg ccagccggta
    42121 aattgttcct cgccgcatac ctgggcgtga tgattatttt ccttgcctcg catatggatg
    42181 cggtggccta caccatggcg gcaaccagta cgcgtaatct ccaggaaggt gacgatcctg
    42241 accgtgggct gcgtcttttc tggtgcgtgg taattaccct gatcccgctt tcaattcttt
    42301 tcactggcgc gtcgctggaa acgatgaaaa ccaccgtcgt actcacagcc cttccgttcc
    42361 tcgtcatttt actggtgaaa gtcggcggat ttattcgctg gctgaaacag gattacgccg
    42421 acattccggc tcatcaagtt gaacattacc tcccgcagac accggttgaa gccctggaaa
    42481 aaacaccagt gctccctgcg ggaaccgtat tcaaaggcga caactgagcg ccatcatcct
    42541 aacgataaag gtatccctat gagcaatctg agccctgact ttgtactacc cgaaaatttt
    42601 tgcgctaacc cgcaagaggc gtggaccatt cctgcccgtt tttataccga tcagaacgcc
    42661 tttgaacacg aaaaagagaa cgtcttcgcc aaaagctgga tttgcgtcgc tcacagcagc
    42721 gaactggcga atgccaatga ttatgtgacg cgtgagatca ttggcgaaag catcgtgctg
    42781 gtacgtggtc gtgataaggt tttgcgcgcg ttctataacg tgtgtccgca ccgtggtcat
    42841 cagttgttga gcggtgaagg aaaagcaaaa aatgtgatta cctgcccgta tcacgcctgg
    42901 gcattcaaac tcgatggcaa cctggcccat gcacgtaact gcgaaaacgt cgccaatttc
    42961 gatagcgaca aagcgcaact ggttccggtg cgtctggaag aatatgccgg attcgtcttc
    43021 atcaacatgg accctaacgc caccagcgta gaagatcagt tacccggtct gggcgcgaaa
    43081 gtgctggaag cctgcccgga agtccacgat ctgaaactgg cggcccgctt taccacccgc
    43141 acgcctgcca actggaagaa cattgtcgat aactatctcg agtgctatca ctgtggtccg
    43201 gcgcatccag gtttctccga ctccgtacag gttgatcgtt actggcacac catgcacggt
    43261 aactggacgc tgcaatacgg tttcgccaaa ccgtcagaac agtctttcaa attcgaagag
    43321 ggcacggatg cggcattcca cggtttctgg ctgtggccgt gcacgatgct gaacgtcacc
    43381 ccgatcaaag ggatgatgac ggtcatttat gaattcccgg tggattctga aactaccctg
    43441 caaaactacg atatttactt caccaatgaa gagttaatcg acgagcaaaa atcgctgatt
    43501 gagtggtatc gcgatgtgtt ccgtccggaa gatttacgtc tggttgaaag cgtacagaaa
    43561 gggctgaaat cgcgtggcta tcgtggtcag gggcgcatca tggccgacag tagcggtagc
    43621 ggaatttccg aacatggtat cgcccacttc cataatctgc tggcgcaggt gtttaaggac
    43681 taatgacatc ggcggcggta ttttccgccg ctgggctgat ttttgatgga gtacagcaat
    43741 gtcagactat caaatgtttg aagtacaggt gagccaaatt gaaccgctta ccgaacaggt
    43801 gaaacgcttc acgctggtgg caaccgatgg caaaccatta cctgcgttta ccggaggaag
    43861 tcacgtcatt gtgcagatga gcgatggtga taacgagtac agcaatgcgt attcactact
    43921 gagttcgccg catgacacct cttgttatca gattgccgtt cggctggagg aaaactcgcg
    43981 cggcggttcc cgctttttgc atcagcaggt aaaattgggc gatcggttaa cgatttcaac
    44041 gcctaataac ctgtttgcgc taattccctc ggcaagaaag catctgttta tcgcgggcgg
    44101 tattggtatc acccctttcc tgtcgcacct ggcagagttg caatacagcg acgtcgactg
    44161 gcagctacat tactgcacgc gaaatccaga aagttgcgca tttcgtgatg agctagtcca
    44221 gcatccacag gctgagaaag ttcacttaca tcactcatca accggaacac gactggaatt
    44281 agcgcgatta ttggcggata tcgaacctgg cacacatgtt tatacctgtg gccccgaggc
    44341 gctaaatgaa gcggtaagaa gtgaagctgc gcgtctgaac atcgccgccg atacgctgca
    44401 ctttgagcaa tttgctatcg aagacaaaac cggcgatgca tttaccctgg tgcttgcccg
    44461 ttccggaaaa gagtttgtgg tgccggaaga gatgactatt ttgcaggtta ttgaaaataa
    44521 taaagcagcg aaagtggaat gtttatgtcg tgaaggggta tgcggaacct gcgaaacggc
    44581 aatactggaa ggtgaagctg accatcggga tcagtatttt agcgatgaag agcgtgccag
    44641 ccagcaaagt atgttgatct gttgttcgcg tgcgaagggt aaacgcctgg tgttggattt
    44701 gtagtttgcc gaagccggac tgagcgcgcc cagtccggct tcggaagatt ttactgcgga
    44761 tattcctgca ataaattgtg taatgcttcc gccatcagct caccacgcca gccggaaatc
    44821 agctccggca aattgttctg cggtttcagt ttccagtgcc agttcagcag ttggttgatt
    44881 tgccgacgcg atgccagcaa ttcggcgctg atcttgtgcg tttcgctcac gtcagtaatc
    44941 aacgacttaa tcgctttaaa cgctttacga taacccggca tgtccatcag gttaagcatc
    45001 ggctgcggta aggcctcttc cggcaatgcc tgcgcttttt ccaccagcgc cagcagcgtt
    45061 ttaccgtgaa agcggatttc gctaccggat aagcccaggc tgtccagttc gcctaaactt
    45121 cccggcatat aacgcgctac ggaccacaaa tgctcttcac gcacgacaaa gttcactgcc
    45181 agatcgcgct cccgcgcctt gcgcagtcgc cagtcggcta acagttgcag acaggccagt
    45241 tggcgtgtgc gtaactgcca ggcattggtg atatcacgcc aggcatcttc cggtgcaacg
    45301 atttcctgac gacgcatttg catcaggcgg cattcatcca gcgccgcagg tagccagccg
    45361 gaggcctccg tttctaccat aagctttgcg gtgatcggta acagatacca gacatccgcc
    45421 gctgcgtatt cacactgacg ttcggtcagc ggtctggcca gccagtcggt gcgcgattca
    45481 ctcttgtcca gcgtaacgcc ggaatactct tccaccatgg aagcgaaacc ccatgacatc
    45541 gggcgtccgc agaaggcagc aaggatttgc gtgtcaatca ggggttgtgg taattcgcca
    45601 aagacattga ggaacacttc cagatcttca ctgcctgcat ggagaaattt tgtgatggac
    45661 ggatcgcgca ggatcgcctt cagtggtgac cagtcggaga tcccgagagg atcaattagc
    45721 gccagatgct caccatcaaa aagttgaatt agacccagtt gcgggtaata ggtgcgcgta
    45781 cgaacaaatt cagtatccag ggctatcgcc ggaaaggcac ggacggcttc acacaaagaa
    45841 gccagcgcat cgtccgtggt aatcatttgg taattcaaat tgtattctct ttagtgggcg
    45901 tcaaaaaaaa cgccggatta accggcgtct gacgactgac ttaacgctca ggctttattg
    45961 tccactttgc cgcgcgcttc gtcacgtaat tctcgtcgca aaatttttcc gacgttagat
    46021 ttcggtaact catcacgaaa ctccaccagc ttcggtactt tgtagcctgt gagctgacgg
    46081 cggcaaaaag tcaccagtga ctcttcggta agcgatggat cttttttcac aacgaagatt
    46141 ttcaccgctt cgccactgga gccagaaggt acgccaacag ccgcgacttc ctgtacgcca
    46201 agatgctgca tgacgacatc ttcaatctcg ttgggataga cgttaaaacc ggaaaccaga
    46261 atcatgtctt ttttacgatc gacaatgcgc aggaatcctt cttcatccat caccgcgatg
    46321 tcgccggtgt gtaaccagcc atttttgatg atttcatcgg tagcatccgg acgctgccag
    46381 taacccagca tcacctgcgg tcctttgaca caaagctcac ccggttgacc cggtggtact
    46441 tcattatcat catcatccac cagtttggct tccgtcgacg gcaccggcaa accgatgcta
    46501 ccactatgat aatcaatatc atacgggtta acgctgacca gcggcgcaca ctcggtcagg
    46561 ccataacctt ccagcagata ctgtcctgtc agtttcaccc aacgttctgc caccacttgc
    46621 tgaacaggca tccctccgcc tgcggaaaga tgcagactgg agaaatccag ctgctggaac
    46681 tctttattgt tcagcaacgc attgaacaag gtgttaacgc cagtgatagc ggtaaacgga
    46741 tatttcgcta actcttttac caaccctgga atatcgcgcg ggttagtgat aagcaggttc
    46801 tgcccaccca gttcgataaa cagcaggcag ttaatggtca gtgcaaaaat gtgatacagc
    46861 ggcagcgccg tcaccaccag ctctttgccc ggatgcaaca gcggaccata ggtcgcgtta
    46921 acctgttcca ggttcgccag catattgcgg tgagtcagca tcgcgccttt cgccacacca
    46981 gtggtaccgc cggtgtattg caggaaagct aaatcttccg gcaccagttc gggtttgacg
    47041 tactgcatcc ggtagccgtt gtgcagggcg ctacgaaatg aaatggcatc tggcagatgg
    47101 tatttcggca ccagacgctt gatgtattta acaacgaaat tgactaccgt gccttttgcc
    47161 gtagatagct gatcgcccat acgggtcaga attacgtgct gaacggcggt tttatcaacc
    47221 actttttcca gagtgtgggc aaagttagac acgataacaa tcgccgatgc gccgctatcg
    47281 ttaagctgat gctcaagttc acgcggggta tacaacgggt taacgtttac gacaatcatc
    47341 ccggcacgca aaatgccaaa cagcgccacc ggatattgca ataaattagg catcatcaac
    47401 gcaacgcgat cgcctttctt cagtcccaac ccttgttgca aataagcggc aaacgcgcga
    47461 ctgcgttctt ccagtttgcg gaaggtcatc acctccccca tattcacaaa cgcgggttga
    47521 tcggcgtagc gcgcgaccga ctgctcaaac atatctacca gagattgata acggtcaggg
    47581 ttgatctccg tcggaacgtc cgcgggataa cggttaagcc aaaccttctt caattcttca
    47641 cctctaaaat gcgtgttcgt cgtcatcgca accccaaatg atatacatgc cgttaacata
    47701 atattaactc atcataccag cttgataatt acccaacgaa aaggttgcga agcgcgtcac
    47761 tatttatttt tatctttacc gtaagaatgc agaaacagcg gaccagccgc tgtttctttt
    47821 tctttaaaaa caagcaatta ttcagttaca acagtttgta ctctcgcggg gccgggatta
    47881 taccagcccc atccaccgta gccatagggc cagccacgac cgccataaaa ccatggatca
    47941 atcggctgag gcggcataat cacctgctgg gttaaatgcc aacgtttgta acccgttacc
    48001 tgcatcacca taaatttata gggcgtatta ccgattttgc cgtcaaccgc accggtgatt
    48061 ggcccaacta ccgtaaccag ttgtccacgg aaatctaccg ggtccagaaa accgttcaca
    48121 tcagcataaa tgcgaccgcg agaaggttct cccagcgtcg gtctggctcc gctgtccagt
    48181 ggtacggtgg caatttccag gcgggttttc ccttgctggt tttgtaccgc aaccactttg
    48241 ccaccaaagc gtgcctcctg accaacgtac agctgcggcg cactcatcac ccgaactaaa
    48301 tcttgttgcg gcgtgggact gctgccttta atggcgtccg gcacagtaac acaaccgctg
    48361 agcattagcg caaacgtacc tgccagtatg cctttgataa cattcttttg aaccgccatg
    48421 atgcgactcc ttttttctca gggcatactc ttaagattca ttctttgccc ggaagtttct
    48481 tccatgcgac gttgttacgt aaataaaccg gttcggcatg ttccaccgcc accgttttac
    48541 cctcggcaaa catctgacac gcaatcggaa gcatatcttc tgcagcgggc agtaacactt
    48601 cgccatcgca cagggtcaaa ccgctctctt taccgagatc cggccaggct tgccagcctg
    48661 tacctaccgt cacccattcc ccggaaagct gttgcatccg ttcatgaacg agttcgggtt
    48721 tgagtacggc ttcggtttct tcaccgtgcc agataccctt ttcatcacgc tgatattcgg
    48781 cccagtaaac ttcgcccatc cgcgcatcaa tagcagccag cacgcgggtt gcgccgtttt
    48841 tgcgccacgc cccttgcgcc attgtcatga gcgtggaaac gccaatcatc ggcagatccg
    48901 cgccaagcgc cagcccctgc gcaataccaa taccaatgcg cacgccggta aagctaccgg
    48961 ggccgcgccc gtaagccaga gcgttaatat cagtcaggga agttccgctg gtggtcagga
    49021 tatcctgcac catcggtaag attcgttgag tatgttcacg agggcaaagc tcaaaatgag
    49081 cgttgacagt accgtcgttc cacagggcga cagagcaggc ctctgtcgcg gtatcgatag
    49141 ccagaattcg catgggtctt cgtgcttaga tcaataaaaa ggcgcgcatc ataccatact
    49201 ccgtaacaaa ttacctggag gatggtatcg caaggaaacg aaccgcacgg gcaatgtcac
    49261 gggtgcgcgg cgctggcggc agactggcga gaaacgtcgc gccgtaaggg cgcatcacca
    49321 gccgattatc gcaaatcacc agcacgccgc gatcgtcggc gtcgcgaatc agtcgcccta
    49381 ctccctgctt gagagtaatc accgcatccg gcagttgtac ttcatcgaac gggtcaccac
    49441 cacgcaaacg gcaatcttcc atgcgcgctt ttaatagtgg atcatccggt gaggtaaacg
    49501 gcaacttgtc gataatcacc aatgacaatg tatcgccacg cacatccacc ccttcccaga
    49561 agctgctggt tgccacaaga agcgcgttac cggcgctgac aaattgctgc aacagttgcc
    49621 ctttgctggt ttccccctgc aacaaaacgg gaagcgtcat ggtagcgcgg aactgctcgg
    49681 caagatcgcg catcatggcg tgcgaggtac aaagcataaa acaacgaccg ttgttagctt
    49741 cgatgatcgg tcgcaacatt gccgccagtt gccgtgcgga gcccggttga ttggtttgcg
    49801 gcaggttgcg cggcacacag agtaacgcct ggcggctgta atcaaacggg ctgggtaaca
    49861 gcaacgactc tgcctgttca atgccaagcc gcgaggtaaa atgatgcaga tcgtcgttca
    49921 ccgacagcgt tgctgaggta aagatccagc taccgggttt ttgcgccatt aactctttga
    49981 atttatccgc cacgctgagc ggcgtgagcg ccagagtaaa atggcgcgaa gtgcattcgt
    50041 accagtagct gtagcccggc tgattgatct cttttagccg tttcagccgc gtacgataca
    50101 acgtggcgcg ctcaaatgcc gcatccagca aggcggaacg ccccagcgac agtttcgcca
    50161 cgtcataaca aagttccagg gtgtcatcga gcagtaaaaa cgcccgttga atttgcggat
    50221 tagctaacag ttcgcgcagg ttgccacgat aaccaggctc accgagttgc agacgaaaat
    50281 cctgcgcgct ctgggcaagg cggtcggcgc acttttgtaa ctgctgagta tcttttaatt
    50341 cggtgcgata ggcgatggtg atgtcttttg ccaggtcgag cagttgtcga ctggagagtg
    50401 actgaccaaa atactggctg gcaatgtcag gcagctggtg ggcttcgtcg aagatcatga
    50461 cgtcagcttc cgggatcagt tcgccaaatc cactctcttt caccaccata tccgccagaa
    50521 agagatgatg gtttaccacc accacatcgg cgtccatcgc ttttttgcgt gctttgacca
    50581 caaagcaatc tttatacatc gggcagtcgc tgccaaggca gttatcgttg gtgctggtga
    50641 ccagcggcca cgcctgtgaa tcttccgcca cgctgacgca ggtgctgata tcaccatcga
    50701 ttgtttgatt agaccaggag cgcagcagga tcacatcgct taagatttgt accggcagat
    50761 cgccccccgt cagcgcctgc tgttcgagac gttcgaggca gaggtagttt gagcgccctt
    50821 tcagcaacgc cacgttgccc gtatatttca acgcctttga aaccgttggc aaatcgcggc
    50881 tgtagagctg atcctgcaac gcttttgagc cggtcgagat aatgactttc tttttcgccc
    50941 gcagcgcagg ggccaggtaa gcgtaggttt tgcccgtacc ggttccggct tccaccacca
    51001 gcggctggcc tttttctatc gcctgggtga cggctaccgc catctgtcgc tgtggttctc
    51061 gcggcttaaa gcctggtatc gctttcgcca gctgaccgtc tggtgcaaaa tcgtccgtca
    51121 cactaccccc tgttgatttg aacagggatt atgtcaggat gagggcgctt tcgccagttg
    51181 aagtggtgac ggcgacctca cattgtggca gtctttgcag cacgaaaatg gaagtattaa
    51241 tgaggacaaa atgatgacta tcgttcgtat cgatgctgaa gcccgctggt ctgatgtagt
    51301 aatccacaac aacacgctct actacactgg tgtaccggaa aacctcgacg ccgatgcctt
    51361 tgagcaaacc gccaacacgc tggcgcagat tgacgccgtg ctggaaaaac agggcagcaa
    51421 taaatcgagc attctggata ccaccatttt cctggccgat aaaaacgact tcgcggcgat
    51481 gaataaagcg tgggatgcct gggttgtcgc gggtcatgcg ccggtgcgct gcacggtaca
    51541 agcgggtttg atgaacccga agtataaagt tgaaattaag attgtggctg cggtgtaagc
    51601 tttatcgaag caaaataagt cagacgataa tttatcgata atactggtcg gttttacata
    51661 aatcgaccag agatt
//
LOCUS       DAPHCV010000017        51390 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.017, whole genome shotgun sequence.
ACCESSION   DAPHCV010000017 DAPHCV010000000
VERSION     DAPHCV010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.017"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(127..1017)
                     /gene="rarD"
                     /locus_tag="RH033_001013"
     CDS             complement(127..1017)
                     /gene="rarD"
                     /locus_tag="RH033_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDV2990237.1"
                     /translation="MDTKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPKKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTAK"
     gene            complement(1069..1536)
                     /locus_tag="RH033_001014"
     CDS             complement(1069..1536)
                     /locus_tag="RH033_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HDV2990238.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGKRFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1701..2570
                     /gene="pldA"
                     /locus_tag="RH033_001015"
     CDS             1701..2570
                     /gene="pldA"
                     /locus_tag="RH033_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HDV2990239.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYNFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2695..4524
                     /gene="recQ"
                     /locus_tag="RH033_001016"
     CDS             2695..4524
                     /gene="recQ"
                     /locus_tag="RH033_001016"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDV2990240.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENDVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4588..5208
                     /gene="rhtC"
                     /locus_tag="RH033_001017"
     CDS             4588..5208
                     /gene="rhtC"
                     /locus_tag="RH033_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HDV2990241.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRSALKKEVVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIIET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5270..5890)
                     /gene="rhtB"
                     /locus_tag="RH033_001018"
     CDS             complement(5270..5890)
                     /gene="rhtB"
                     /locus_tag="RH033_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HDV2990242.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKPL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6001..7023
                     /gene="pldB"
                     /locus_tag="RH033_001019"
     CDS             6001..7023
                     /gene="pldB"
                     /locus_tag="RH033_001019"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HDV2990243.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7031..7831
                     /gene="yigL"
                     /locus_tag="RH033_001020"
     CDS             7031..7831
                     /gene="yigL"
                     /locus_tag="RH033_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HDV2990244.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFNYALYEPGLLEPEGVSKVFFTCDSHEKLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLQDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNAEDAVPHYLRKLYLS"
     gene            7907..8806
                     /gene="bioP"
                     /locus_tag="RH033_001021"
     CDS             7907..8806
                     /gene="bioP"
                     /locus_tag="RH033_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HDV2990245.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNTQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALNE"
     gene            complement(8694..9647)
                     /gene="metR"
                     /locus_tag="RH033_001022"
     CDS             complement(8694..9647)
                     /gene="metR"
                     /locus_tag="RH033_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HDV2990246.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9765..12026
                     /gene="metE"
                     /locus_tag="RH033_001023"
     CDS             9765..12026
                     /gene="metE"
                     /locus_tag="RH033_001023"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDV2990247.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNNDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHKVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLNAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            12365..12922
                     /locus_tag="RH033_001024"
     CDS             12365..12922
                     /locus_tag="RH033_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDV2990248.1"
                     /translation="MDAKLYITEIINELTFVKDSIREDDCTKLIDAIQQSQRVFCYGL
                     GRAGFSMKAFTMRLMHMGKEVYFLTETITPNFGPGDLFIVSSASGETAQLVALAKKAR
                     QLGGAVAVLTTNRHATITEFVDVIVQINAPSKNQKDSVFRSAQPMASLYEQALLVIAD
                     ALVMKMAAESGAPESELFKRHANLE"
     gene            12964..14472
                     /locus_tag="RH033_001025"
     CDS             12964..14472
                     /locus_tag="RH033_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDV2990249.1"
                     /translation="MSKKITSLFNFKKLQKFSSALMLPIAILPAAGLILAIGISFNIE
                     LLANCGGLIFANLPLLFCVGIVIGLTGDGSSSLSAIINYMIMNATMGTQLGITAESVA
                     ASNGDYAMILGVPTLQTGVFGGLIVAIFVYLLYTRFHNIELPQFLGFFGGKRFVPIIT
                     AVAAIIVGYLLPWLWITVQKGLASLSTLVTSGHNANFAAFIYAVGERSLIPFGLHHIW
                     NVPFYYNFGDYMTKSGQLVTGDIPVFFAQLRDGVPLTAGLFMTGRFPIMMFALPAAAL
                     AMYQEAKPERKALIKGLLLSGALTTFVTGITEPLEYAFLFAAPVLYIIHIFLYATSFV
                     LMNILNVHIGHPFAGGLIDFVLNGVMPNRTPWYLVFLAGAGYAAIYYTVFRVLIRKLN
                     LKTPGREDEEIAVATTLTREERPYQIIEAVGGFDNIEDVDACATRLRLALVDDKKVNE
                     KRLKELGAAGLVKLGDGGVQVIFGGKSQILRDEIKTVMSRPRPTEMTVACAN"
     gene            14523..16520
                     /gene="tkt"
                     /locus_tag="RH033_001026"
     CDS             14523..16520
                     /gene="tkt"
                     /locus_tag="RH033_001026"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDV2990250.1"
                     /translation="MNSQLLANAIRMLSVDAIQKANSGHPGAPMGMADIAEVVWRRHL
                     RHNPKNPQWFNRDRYVQSNGHGSMLIYALLHLTGYDLSMDDIRDFRQLHSRTPGHPEY
                     GYTPGVETTTGPLGQGVANAVGMAIAEKALAAEFNKPGFNIVDHHTWLFLGDGCLMEG
                     ISHEACGLAGTLKLGNLIAIWDDNGISIDGHVEGWFAEDTAARFRAYGWHVIEGVDGH
                     DPEAVDAAVREAKSVTDKPSLLCCKTIIGFGSPNKANSHDCHGSALGADEVALVRERL
                     QWPYAPFEIPGEIYAEWDATEKGAQVQQEWDALFADYAKQWPELAAEFTRRMKGDLPA
                     GWAENMQKYVHDLQSHPAALATRQVSQKCLNHFADMLPELMGGSADLSPSNLTRHQKS
                     VDFTGENPAGNYISYGVREFGMSAIMNGLALHGGFIPYGGTFLMFMEYARNALRMAAL
                     MKIRSVFVYTHDTIGLGEDGPTHQPVEQLASLRLTPNMETWRGCDQVEVAVAWQQAIE
                     RKDGPTSLVLTRQPLAQQPRTAAQLAEIARGGYVLSDCDGQPEMILISAGSEIELVVS
                     AAKALTEEGRKVRVVSLPCTERFDNQDAAYKESVLPKAVRKRLAVEASIAGFWERYVG
                     LDGKVIGMTSFGESAPANVLFKHFGFTPENVLAQARELLNS"
     gene            complement(16550..17395)
                     /locus_tag="RH033_001027"
     CDS             complement(16550..17395)
                     /locus_tag="RH033_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDV2990251.1"
                     /translation="MNNITVKSLIQSNYPTLHQAEKKVADYILSHAHEVVNYSVTELS
                     EKSHASEATIVRTCKKLGYQGYYHLKIALAKEVINPDNSYPDNTDFTDITSLATFLLK
                     KQAEDLIQSTQFFNAEVLESILKLLANCDTIFFFAAGNSNPLAVYGAYKFSQLGLKTV
                     VHVSPEMQINAAYSMGKRDLAIGISNSGSTNLMVDIFKVVKERGAKSVCITNYIKSPL
                     SKLSTHQLNTAVSDKIFFEAFDSTRVPAMGVIDMLVLLFMYKNKAHYEKYRTREEFLG
                     SRFKG"
     gene            complement(17596..18501)
                     /locus_tag="RH033_001028"
     CDS             complement(17596..18501)
                     /locus_tag="RH033_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2990252.1"
                     /translation="MFISDETLRVIHLVARHQSITTAAEQLNKVPSAISYTIKKAEES
                     LGVELFIRKGRYIELSPAGSYFIEHSKTILGDLEALKRNTVLIHDGIERELTIAVNNI
                     IPGDLLVAFIRDFERQFTSTTLTVDLEVYNGCWDALYSKRADLVYGAPHAVPSSEGII
                     SEPVGQMEWDFVVSPLHPLAAKRHPLENSELRHYPAVCIRDTSVNFPPMQAWLLEGQK
                     PIFVPDFATAIALIEQNVGIGYIPHHLALPLLNSGKLLKKPMREHKHATKLFLAARSD
                     GMGKACQWCIEYLRNPQLMTRFVFN"
     gene            complement(18579..19802)
                     /locus_tag="RH033_001029"
     CDS             complement(18579..19802)
                     /locus_tag="RH033_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDV2990253.1"
                     /translation="MRKKEENLNTASGLRIAMILLGIAVTPVLLSSSSLGNQLSSGSL
                     ISVVLLGGVILTLLSAITISVGEKARLPTYGIVKYSFGEKGAIAINILMAISLFGWIA
                     VTANMFGHSVHDLLAQHGLEVPLALLVAAGCVIFVASTAFGFAVLGKIAQVAVPVIAL
                     VLCYILYVATHTEVAVPAAIVEMNTGVAVSTVVGTIIVLVATLPDFGSFVHNRKHALI
                     AAGVTFLVAYPLLYWAGATPSAISGQGSLLGAMAVFGAVLPAALLLIFACVTGNAGNM
                     FQGTLVVSTLLTRFPKWQITVALGILSAIVGSMDIMAWFIPFLLFLGIATPPVAGIYI
                     ADFFLYRRNGYQESVLAQESQIKVLTFAAWIIGAAVGFMTVKGLFTLTTIPSVDSILV
                     ACIAYAILSRASQHR"
     gene            complement(19828..20217)
                     /locus_tag="RH033_001030"
     CDS             complement(19828..20217)
                     /locus_tag="RH033_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA amidotransferase"
                     /protein_id="HDV2990254.1"
                     /translation="MTTTLLAVNGTLMRGLELNPNMQKAGGIFVREDRTDAHYRLWSI
                     NDRHPGMIRVNEGGTHVDVEIWQLPLASFAALLMSEPAGLAIGKIKLADGSEVLGVLA
                     ENWLTEGQREITELGSWRKYTGHFHTV"
     gene            complement(20234..21190)
                     /gene="arcC"
                     /locus_tag="RH033_001031"
     CDS             complement(20234..21190)
                     /gene="arcC"
                     /locus_tag="RH033_001031"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDV2990255.1"
                     /translation="MVKPMAVVAVGGNALIQDEQRNSIPDQYVAVMESVQHIVDMVEA
                     GWDLVLTHGNGPQVGFILRRSELASNEVSPVPLDYAVGDTQGAIGYMFQKALHNELAR
                     RGINKPVIALVTQTRVSPHDDAFASPSKPIGAFLDEATAQQRQQQLGWTLMEDAGRGW
                     RRTVPSPAPLEIIEHDTIAHLVRQGYLVIACGGGGIPVVRDGQQLKGVEAVIDKDLAS
                     ALLASQLGADLLVIPTGVEKVAINFGTPQQQWLDAISIAEAQTLLREGQFGVGSMQPK
                     VEAIVDFINASQQQGKQASGLITSPQTIKAALAHQSGTWITL"
     gene            complement(21183..22607)
                     /locus_tag="RH033_001032"
     CDS             complement(21183..22607)
                     /locus_tag="RH033_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HDV2990256.1"
                     /translation="MNTLFNQPLKVVNAGLHSFADNIQHAGGSAIALNWQPPAQGDID
                     AGLDLASLLRHPLVENANQIAMTRYLEAQPMLVDVMLAKEAIPAMAEQKRILHSGPPI
                     AWEEMCGPVKGAIIGAMLYEGWATSQQDAENQINAGEIDLAPCHHYHAVGPMAGIISP
                     SMPLWVVENKTNGHRTFSNFNEGLGKVLRFGANNDEVLNRLAWMRDELAPAMKAAIAQ
                     HGELELKPLMAQALHMGDEVHNRNAAATGLLIKRLLPALLACSLPQEHIQRVVAFITG
                     NDHFFLNLSMAACKAMMDAAANVPFSSMVTVMARNGVNFGIRLSGTGDRWFQAPANAV
                     EGLFFPGFGVDDAAADLGDSAITETAGVGGFAMASSPAIVKFVGGTPADATNNSRRMQ
                     AITLGGNPAFTLPALNFAPTAAGIDARKVTDRGILPVINTGIAHKQAGVGQIGAGITT
                     APMACFVAAVRALAEIVAKENHHG"
     gene            complement(22604..24163)
                     /gene="fdrA"
                     /locus_tag="RH033_001033"
     CDS             complement(22604..24163)
                     /gene="fdrA"
                     /locus_tag="RH033_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDV2990257.1"
                     /translation="MKVKYKVFSNLYQDSVSLMQISAQISKLPGIQQASVVMGTPNNL
                     EQLRDAGLGNEINASPNDLVIAVMGEEDICNEALILAQQRLTSKPDDETGSGIKTPEK
                     VSLEMALEAEPEANLALISVPGDYAAAEAIKALNLGMNVMMFSDNVSIAQEKSIKTLA
                     RERQRIVMGPDCGTAIVNGIPLGFANVVKRGAIGVIGASGTGLQEVTCRIDQLGVGIS
                     QALGTGGHDLSEEIGGISMLFALDALAQDDETRVIVLISKPPSPIVARTILERAEACG
                     KPVVVNFLGANPHDLARPNITAATTLASAADIAVALLNVQPLPAVETDVPCDDLTMLQ
                     NACQRLPAHRQAIRGVFAGGTFCYEAQLICQQKGFSAASNTPVAGNRALANIWQSEDH
                     TLIDMGDDDFTRGKPHPMIDPTLRNQRLLNELNDSHTAVVLFDLVLGYGASTTPASEL
                     LDQLSHIDMNNAPLLIAHVCGTEADPQIRSQQIRALQNAGVIIASSNAQAALWASTVA
                     QTQLQKKGLNA"
     gene            complement(24258..25118)
                     /locus_tag="RH033_001034"
     CDS             complement(24258..25118)
                     /locus_tag="RH033_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDV2990258.1"
                     /translation="MVQVTLCASSLGYLFPQNETQDLRLHSAFKHAVNLYSDAGTFIT
                     LLCAQTYLNLPDAARVWLPECWDWRREIAHSDPIQLTPGLLRTPRFCVALDNATLWQS
                     PFVGGMLTLETFPLVFQHYPTMASQRLLFCLEHNVQSTLHLPDSLTHQGLAIMEHPDA
                     LERQVPQLIGFGKGLTPDGDDYLLGYLAALWLWQLPAPLADHQYRLQQAIDQHAHNTT
                     DISRHYLERALQGHFSEPICQLLAQLVGSASAMTIASCAEQVMQFGATSGVDCLAGML
                     HGFRTLNTMN"
     gene            complement(25124..25792)
                     /locus_tag="RH033_001035"
     CDS             complement(25124..25792)
                     /locus_tag="RH033_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDV2990259.1"
                     /translation="MTQSIFQAQPFELPFDPRTTALVMIDMQRDFVEAGGFGEALGND
                     VSRVRTAIAPCTEVLAAARQKGIMVIHTREGHRADLSDCPPAKLTRGGKTFIGEPGPM
                     GRILVRGEAGHDIIPELYPVAGEPVIDKPGKGAFYQTDLHLILQNHGIKTLIVCGVTT
                     EVCVTTTVREANDRGYECIIPEDCVGSYFPEFQKYALEMIKAQGAIFGWVTDSKAIIA
                     GLEG"
     gene            complement(26049..26858)
                     /locus_tag="RH033_001036"
     CDS             complement(26049..26858)
                     /locus_tag="RH033_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDV2990260.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFTQYGVKK"
     gene            27120..27884
                     /gene="udp"
                     /locus_tag="RH033_001037"
     CDS             27120..27884
                     /gene="udp"
                     /locus_tag="RH033_001037"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HDV2990261.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLLK"
     gene            complement(27918..29408)
                     /locus_tag="RH033_001038"
     CDS             complement(27918..29408)
                     /locus_tag="RH033_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HDV2990262.1"
                     /translation="MFESELLTQGFSTLLNNPQALLFATFGVMLGIVIGALPGLTATM
                     GVAILLPFTYGMEPVSGLLMICGVFFGGVYGGSITAILLKIPGTPAAAATAIDGYELT
                     KQGKAGLALSAATFSSFSGGTLSIIVLMFLSPVLASWALKFSASESFALATFGLSIIA
                     SISGESLIKGLIAGVGGLLIATIGLDPMGGFPRFTGGFVELMNVPFIPVMIGLFAASE
                     AFRSMEQNQQIRQGAKVAIGSLLLPWQTLRRIALTILRSSGLGVFIGMIPGAGADIAA
                     FVAYNETRRFSKTPENFGKGEIKAVASCEAGANGCTGGALLPMLTLGIPGDAVTAIML
                     GALTLQGMQPGPLMFTDHGDMVYTLFVGMIFCYFMLLVLGLLSLKVIGNVVKIPGNIL
                     TPMILALCVVGTYALNNSLFDVGIMLIAGVVGYFMQKGGYPASPVVLALIMGPMAESN
                     FRRALSLSGGSLDFLYTRPITLALLTLAAFTLLTPIIRKIMRLRRQ"
     gene            complement(29421..29927)
                     /locus_tag="RH033_001039"
     CDS             complement(29421..29927)
                     /locus_tag="RH033_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HDV2990263.1"
                     /translation="MNTKQSVAQLAVPHRKRLSSTMVVALLLCVVAGAVMINAADFPA
                     TAIETDPGASAFPTFYACALIVLAVLLVIRDLLQAKPASCANAQEKPAFRKTATGIAA
                     TAFYIVAMSYCGYLITTPVFLIVIMTLMGYRRWVLTPGIALLLTAILWLLFVEALQVP
                     LPVGTFFE"
     gene            complement(29944..30918)
                     /locus_tag="RH033_001040"
     CDS             complement(29944..30918)
                     /locus_tag="RH033_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HDV2990264.1"
                     /translation="MIKHLTLPKKMAAMVLLAGAALSIAPVQAASYPTKQIELVVPYA
                     AGGGTDLVARAFADAAKNHLPVSIGVINKPGGGGAIGLSEIAAARPNGYKIGLGTVEL
                     TTLPSLGMVRFKTSDFKPIARLNADPAAITVRADAPWNSYEEFMAYSKANPGKVRIGN
                     SGTGAIWHLAAAALEDKTGTKFSHVPYDGAAPAITGLLGGHIEAVSVSPGEVINHVNG
                     GKLKTLVVMADERMKTMPDVPTLKEKGVDLSIGTWRGLIVSQKTPQDVVDVLAKAAKE
                     TAEEPAFQDALQKLNLNYAWLDAASFQTQISEQEKYFDELLTRLGLKK"
     gene            complement(30943..31572)
                     /locus_tag="RH033_001041"
     CDS             complement(30943..31572)
                     /locus_tag="RH033_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001116470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HDV2990265.1"
                     /translation="MPFNTLLQKTGLVAILRGVKPDEIVAIGEKLYAAGFRLIEIPMN
                     SPEALQSISLLRDALPKDCLVGAGTVLSVEQVVAVKEAGGQIIVMPHCDTAVIRRARA
                     LGMYCAPGVATPTEAFAAIEHGANAIKLFPAEQITPEVTKAWRAVIPQSVPMLPVGGI
                     TPETMARYLSHGANGFGLGSALYRPGMTPEQVYENAVLFMNAWNNLNSN"
     gene            complement(31562..32566)
                     /locus_tag="RH033_001042"
     CDS             complement(31562..32566)
                     /locus_tag="RH033_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HDV2990266.1"
                     /translation="MPGAPSFIALDWGTSSLRAWRFGESPNPQEKREFPWGIMKLPSQ
                     AATREDAFHDTFLRVCGDWLAQTPCPVLACGMLGSAQGWQSAAYLPCPVTLEGLAKQL
                     TPVIHQQQTMLHIIPGVIKEGEMPEVMRGEETQIFGAISMEPALQNAIHQGMPVLIGL
                     PGTHAKWAVVENNTITDFRTFMTGELFDVLSRHSILGATMHPGDEPHWDAFTHGLTAA
                     QEHHQTGLLSTLFSTRSRLLTSNLTSSSQGDYLSGLLIGHELCGLASSLLRDLPATTP
                     IALIGSANLNSRYSQAFSHVFPDRQIHAIPNATEHGLWRIAHAAGLLSTNARECTHAI
                     "
     gene            complement(32590..33357)
                     /locus_tag="RH033_001043"
     CDS             complement(32590..33357)
                     /locus_tag="RH033_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDV2990267.1"
                     /translation="MAETSSTTAGAQTLLRGLAVLNAVYNGCHDLKSIGEFTGTTRST
                     THRLVTVLVEQRYLRHVPTQGYQLGAKLIEFGARALESTSLYEIAQPVLQRLARYTLD
                     TVHLGIVEGDEVLYLEKINSQRGLEMRSRPGHRMPLAITGIGKALILNRTEEEWRTLF
                     KTCGDETKLGAFIQNMRRYAASGFAFDLEENEPTIRCVAAPVYNARDEIVAAISVAST
                     TTYMSLARLEELAPYVKSCAEEISAELGWGKHVRKDK"
     gene            33574..34944
                     /gene="rmuC"
                     /locus_tag="RH033_001044"
     CDS             33574..34944
                     /gene="rmuC"
                     /locus_tag="RH033_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HDV2990268.1"
                     /translation="MDFSIIVYAIIALVSVVIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFVDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVP"
     gene            35096..35851
                     /gene="ubiE"
                     /locus_tag="RH033_001045"
     CDS             35096..35851
                     /gene="ubiE"
                     /locus_tag="RH033_001045"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HDV2990269.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKTMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            35865..36470
                     /gene="ubiJ"
                     /locus_tag="RH033_001046"
     CDS             35865..36470
                     /gene="ubiJ"
                     /locus_tag="RH033_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HDV2990270.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            36467..38107
                     /gene="ubiB"
                     /locus_tag="RH033_001047"
     CDS             36467..38107
                     /gene="ubiB"
                     /locus_tag="RH033_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HDV2990271.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKPLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            38186..38455
                     /gene="tatA"
                     /locus_tag="RH033_001048"
     CDS             38186..38455
                     /gene="tatA"
                     /locus_tag="RH033_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HDV2990272.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            38459..38974
                     /gene="tatB"
                     /locus_tag="RH033_001049"
     CDS             38459..38974
                     /gene="tatB"
                     /locus_tag="RH033_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HDV2990273.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            38977..39753
                     /gene="tatC"
                     /locus_tag="RH033_001050"
     CDS             38977..39753
                     /gene="tatC"
                     /locus_tag="RH033_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HDV2990274.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            39795..40577
                     /gene="tatD"
                     /locus_tag="RH033_001051"
     CDS             39795..40577
                     /gene="tatD"
                     /locus_tag="RH033_001051"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HDV2990275.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARALDAGVNGLFITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(40574..41062)
                     /gene="rfaH"
                     /locus_tag="RH033_001052"
     CDS             complement(40574..41062)
                     /gene="rfaH"
                     /locus_tag="RH033_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HDV2990276.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            41229..42722
                     /gene="ubiD"
                     /locus_tag="RH033_001053"
     CDS             41229..42722
                     /gene="ubiD"
                     /locus_tag="RH033_001053"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HDV2990277.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            42768..43469
                     /gene="fre"
                     /locus_tag="RH033_001054"
     CDS             42768..43469
                     /gene="fre"
                     /locus_tag="RH033_001054"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HDV2990278.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(43661..44824)
                     /gene="fadA"
                     /locus_tag="RH033_001055"
     CDS             complement(43661..44824)
                     /gene="fadA"
                     /locus_tag="RH033_001055"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HDV2990279.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRHVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISSTLLNLMERKDAQFGLATMCIGLGQGIATVFERI"
     gene            complement(44834..47023)
                     /gene="fadB"
                     /locus_tag="RH033_001056"
     CDS             complement(44834..47023)
                     /gene="fadB"
                     /locus_tag="RH033_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HDV2990280.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEAIMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKRAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYSGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAVVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKMA"
     gene            47213..48544
                     /gene="pepQ"
                     /locus_tag="RH033_001057"
     CDS             47213..48544
                     /gene="pepQ"
                     /locus_tag="RH033_001057"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HDV2990281.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            48544..49158
                     /locus_tag="RH033_001058"
     CDS             48544..49158
                     /locus_tag="RH033_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HDV2990282.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPTGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            49197..50648
                     /gene="trkH"
                     /locus_tag="RH033_001059"
     CDS             49197..50648
                     /gene="trkH"
                     /locus_tag="RH033_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HDV2990283.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            50660..51205
                     /gene="hemG"
                     /locus_tag="RH033_001060"
     CDS             50660..51205
                     /gene="hemG"
                     /locus_tag="RH033_001060"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HDV2990284.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT        12197 a        12973 c        13721 g        12499 t
ORIGIN      
        1 aagcaaatga aaatatacaa cgacgtctgg aaattctgcc tttccttatg tgagtctcct
       61 gtcagcatgg caatatgccc actctcatgc aagagtcggc atattttttc agaatatatt
      121 tattttttat ttggccgttc tacgctgcgt ataaatcgca tccatcacaa aaattgccag
      181 cgccacccaa ataaaggtga aagtcaccat cttatcggca cccggttttt caccataaaa
      241 cgtcactgcc aacaggaaca ttagcgtcgg cccaatgtac tggaaaaagc ctaatgttga
      301 gagtcgtaag cgtgtagctg ctgcggtaaa acataacagt ggcacggtgg tgacaatacc
      361 ggcggcgatc agcagtaaat tcagcgacat cgggttttgc cccatatggc tggttgagct
      421 gtcggcaata gcaaacaggt aaatcgccgc cacgggcagc agccacatgg tttcgattaa
      481 catgccggtt tgcgcttcaa cggcaatttt cttgcgtacc aggccgtaga aggcaaaact
      541 aaacgccagc cccagcgcga tgattggcag cgaaccaaaa gtccagagct ggaccaatac
      601 accacatatc gccagaatca ccgccagcca ttgcatccgg cggaatcgct cgccgaggaa
      661 aatcatcccc agcacaatgt tcaccagcgg gttaataaag taaccaaggc tcgcttccag
      721 catatggtga ttgttcaccg cccagataaa cagtagccag ttgccaccaa tcagcacggc
      781 agagactgcc agcatgaaga ttttctttgg tgtctgaatc agcgttttta aataggacca
      841 ctggcggcaa atgctcatca gcaccaccat aaagaaaaac gaccagatca cgcgatgcgt
      901 caggatctca tcggcgggca cgtagtaaat cagcttgaag tacgctggcg caatacccca
      961 aataaaataa gcggcaagag cgagtaatac gccctgccgc gtttgtttgg tgtccattgg
     1021 caaaactcat tttttattgg taaaagcagt ttacctgctt ttacgttatc aacctaccat
     1081 ataggtggcg gtggcactgg ctatataaag ctgttcttca ttgtgtaatt caacgcgggc
     1141 aacggcgact ttattgcctg cacgcaacag actactggta gcagtaaaac gcttgcccct
     1201 gcctgggcgc agataatcga cgcgaagatc aatggtcccc atccgcgata gccgctggcg
     1261 tagttcatct tcactgatgg tttcgtggcg ggttaaggta cttcccacgc acaccagacc
     1321 tgcggcgaca tccagcgccg acgcaatgac cccgccgtgc aaaatgcttt gcgcccagtt
     1381 acccaccatc attggctgat ttttaaaggc cagctgtgcg aactcttttt cgtaacgctc
     1441 cagttccatc cccaatgcgc ggttaaatgg catgtgataa acaaacatct cacccactaa
     1501 tttcagggct tgttcagcgg tcagtacggc agacatatca tccttacacc tcattggtta
     1561 atgaaatgtt gattttatgc ttcttaattg tggttttcta ctttaggaag ggataactaa
     1621 cggcaaagga gttaagtatg taaaatagcc cgcagaaaaa tattcatcta atcaataatt
     1681 cgttacggag aacacgaccg atgcggactc tgcagggctg gttgttgccg gtgtttatgt
     1741 tgcctatggc agtatatgca caagaggcaa cggtgaaaga ggtgcatgac gcgccagcgg
     1801 tgcgtggcag tattattgcc aatatgctgc aggagcatga caatccgttc acgctctatc
     1861 cttatgacac caactacctc atctacaccc aaaccagcga tctgaacaaa gaagcgattg
     1921 ccagttacga ctgggcggaa aatgcgcgta aggatgaagt aaagtttcag ttgagcctgg
     1981 catttccgct gtggcgtggg attttaggcc cgaactcggt gttgggtgcg tcttatacgc
     2041 aaaaatcctg gtggcaactg tccaatagcg aagagtcttc accgtttcgt gaaactaact
     2101 acgaaccgca attgttcctc ggttttgcca cggattacaa ctttgctggc tggacgctgc
     2161 gcgatgtgga aatgggctac aaccacgact caaacgggcg ttcagacccg acttcccgca
     2221 gctggaaccg cctttatacc cgcctgatgg cagaaaacgg taactggctg gtagaggtga
     2281 agccgtggta tgtggtgggg aatacggacg ataacccgga catcaccaaa tatatgggct
     2341 attaccagct taaaatcggc tatcacctcg gtgatgcggt actgagcgcg aaagggcagt
     2401 acaactggaa caccggttac ggtggtgcgg agttaggctt aagttacccg atcaccaaac
     2461 atgtgcgcct ttatactcag gtttacagtg gttacggcga atcgctcatc gactataact
     2521 tcaaccagac ccgcgttggt gtgggagtta tgctaaacga cctgttttga tgaatggttg
     2581 tcaaatctgg aatccagtat ccaggattac cctctcagag actaaaagca ttgcagtttc
     2641 tcgcgcaggc gctgaaaata gcgcctgttt ttatttcagg caatcggggt gaatgtggcg
     2701 caggcggaag tgttgaatct ggagtccgga gctaaacagg ttttacaaga aacctttggc
     2761 taccaacagt ttcgccccgg ccaggaagag attatcgaca ctgtgctttc cggtcgcgat
     2821 tgcctggtcg tcatgcccac cggtggcgga aaatcccttt gctatcaaat tcctgcctta
     2881 ttgctaaacg gccttaccgt ggttgtttca ccgctgattt cgttgatgaa agatcaggtg
     2941 gatcaactgc aagccaacgg cgtggcggcg gcgtgcctta actcgacgca aacccgcgaa
     3001 cagcaacttg aagtgatgac aggctgtcgc accgggcaaa ttcgtctgct ttatatcgcc
     3061 ccggaacgcc tgatgctgga taactttctt gagcatctgg cgcactggaa tccggtgtta
     3121 ttagccgttg atgaagcgca ctgtatctcc caatggggcc acgatttccg cccggaatat
     3181 gccgcgctcg gtcagttgcg ccagcggttc ccgacgctgc cgtttatggc gctgaccgcc
     3241 accgccgacg acaccacgcg gcaggatatc gtgcgcctgc tggggctgaa cgatccgctg
     3301 attcaaatta gcagttttga ccgtccgaat attcgctaca tgttgatgga gaagttcaaa
     3361 ccgctcgatc agctgatgcg ctacgtgcag gaacaacgcg gtaagtcggg gattatttac
     3421 tgtaatagtc gtgcgaaagt agaagacacc gccgcgcgtc tgcaaagcaa gggtatcagc
     3481 gcagcggctt atcacgccgg gcttgaaaac gatgtccgtg cggacgtgca ggaaaaattc
     3541 cagcgcgatg acctgcaaat tgtggtggcg acggtggcgt tcggcatggg catcaataaa
     3601 ccaaacgttc gcttcgtggt gcattttgat attccgcgca acattgaatc ctattatcag
     3661 gaaaccggac gcgccgggcg tgatggcctg cccgcggaag cgatgctgtt ttacgatccg
     3721 gcggatatgg cgtggctgcg tcgttgtctg gaagagaaac cgcaggggca gttacaggat
     3781 atcgaacgtc ataaactcaa tgcgatgggc gcgtttgccg aagcgcaaac ttgccgtcgt
     3841 ctggtattgc tgaactattt tggcgaaggg cggcaggagc cgtgcgggaa ctgcgatatc
     3901 tgcctcgatc cgccgaaaca atacgatggt tcaaccgatg ctcagattgc cctttccacc
     3961 attggtcgtg tgaatcagcg gtttgggatg ggctatgtgg tggaagtgat tcgtggcgct
     4021 aataaccagc gtatccgcga ctatggtcat gacaaactga aagtctatgg catgggccgt
     4081 gataaaagcc atgaacattg ggtgagcgtg atccgccagc tgattcacct cggcctggtg
     4141 acgcaaaata ttgcccagca ttctgcccta caactgacag aggctgcgcg tccggtgctg
     4201 cgcggcgaat cctctttgca acttgccgtg ccgcgtatcg tggcgctcaa accgaaagcg
     4261 atgcagaaat cgttcggcgg caactatgat cgcaaactgt tcgccaaatt acgcaaactg
     4321 cgtaaatcga ttgccgatga aagcaatgtc ccgccgtacg tggtatttaa cgacgcaacc
     4381 ttgattgaga tggcggaaca gatgccgatc actgccagcg aaatgctcag cgttaacggc
     4441 gttgggatgc gcaagctgga acgctttggc aaaccgttta tggcgctgat tcgtgcgcat
     4501 gtcgatggcg atgacgaaga gtagtcagca ggataaaaaa gtgccaggat gaagactccg
     4561 taaacgtttc ccccgcgagt ctaatgtatg ttgatgttat tcctcaccgt cgccatggtg
     4621 cacattgtgg cgcttatgag ccccggtccc gatttcttct ttgtttctca gaccgctgtc
     4681 agtcgttccc gtaaagaggc gatgatgggc gtgctgggca ttacctgcgg cgtaatggtc
     4741 tgggcaggga tcgcgttgct tggcctgcat ttgattatcg aaaaaatggc ctggctgcat
     4801 acgctgatta tggtgggcgg tggcctgtat ctctgctgga tgggttatca gatgctgcgt
     4861 agcgcactga aaaaagaggt cgtttctgcg cctgcgccac aggtagaact ggcaaaaagc
     4921 gggcgcagtt tcctgaaagg cttactgacc aatctcgcca atccgaaagc aattatctac
     4981 tttggttcgg tattctcgct gtttgtcggc gataacgtcg gcaccactga gcgctggggc
     5041 attttcgcgt tgattattat cgaaacactg gcatggttta cggtggttgc cagcctgttt
     5101 gctctaccgc aaatgcgccg tggttatcaa cgtctggcga agtggattga tggttttgcc
     5161 ggggcgttat ttgccggatt tggcattcat ttgattattt ctcgttgatg ccaaacgctt
     5221 catcagagta agtcggataa ggcgtttatg ccgcatccga cattattttt cacgcatgcc
     5281 tcgccgatgc caacaacgct cctaccagca taaacagcga gccaaaaatc ttattcaatg
     5341 ccttcatctg tttcggccct tttatccata acgcaatccg ttgagcaagg gtggcgtaac
     5401 cgatcatcac aataatatcg accacaatag tggtgacgcc gagcacgata tactgcatca
     5461 gttgtggctg ctgcggcatg atgaattgcg gaaatagcgc cgccagaaac acaatacttt
     5521 tgggattggt gagattgaca aaaactgcgc gctggaacaa atgtcgacgc gattgagtag
     5581 aggccagcgg tttaagatca attgcgccag cggcgcgcca ctgctggatc ccaagccaaa
     5641 tcaagtaagc tgcgcctgcc cacttcaaca cttcaaacgc aatcactgag cgggaaaata
     5701 gcgtccccaa ccccacgcca accagcacaa tatgaatcgc cagtccggtc tgaagcccag
     5761 caatagacgc caccgcgccg cgataaccgt ggttgagcga ggtggtcatg gtgttgattg
     5821 cgccagagcc tggcgacagg cttaaaatga tcgatgtcag caggtaggca aaccaccatt
     5881 ctaaggtcat gatgaactcc cggtgtgtct atttttgtgc cacaatacgc tactgtcgca
     5941 gcgttgtgtc aggcacgctt aaaaaaacga ttttacgtgg tttaagaggc agattacccg
     6001 atgtttcagc agcaaaaaga ctgggaaaca agagaaaacg cgtttgctgc ttttaccatg
     6061 ggaccgctga ctgatttctg gcgtcagcga gatgaagcag agtttactgg tgtggatgac
     6121 attccggtgc gctttgtccg ttttcgcgca cagcaccatg acagggtggt agtcatctgc
     6181 ccggggcgta ttgagagcta cgtaaaatat gcggaactgg cctacgacct gttccatttg
     6241 gggtttgatg tcttaatcat cgaccatcgc gggcagggac gttccggtcg cctgttagcc
     6301 gatccgcatc tcgggcatgt taatcgcttt aatgattatg ttgatgatct ggcggcattc
     6361 tggcaacagg aggtccagcc cggtccgtgg cgtaaacgct atatactggc acattcgatg
     6421 ggcggtgcga tctccacatt atttctgcaa cgccatccag gtgtatgtga cgccattgcg
     6481 ctaactgcgc caatgtttgg catcgtgatt cgtatgccgt catttatggc acggcagatc
     6541 ctcaactggg ccgaagcgca tccacgtttc cgtgatggct atgcaatagg caccgggcgc
     6601 tggcgcgcgt tgccttttgc tatcaacgta ctgacccaca gcagacagcg ctatcgacgt
     6661 aacttacgct tctatgctga tgacccaacg attcgcgtcg gtgggccgac ctaccattgg
     6721 gtacgcgaaa gtattctggc tggcgaacag gtgttagccg gtgcgggtga tgacgccacg
     6781 ccaacgcttc tcttgcaggc tgaagaggaa cgcgtggtgg ataaccgcat gcacgaccgt
     6841 ttttgtgaac tccgcaccgc tgcgggccat cctgtcgaag gaggacggcc gttggtaatt
     6901 aaaggtgctt accatgagat cctttttgaa aaggacgcaa tgcgctcagt cgcgctccac
     6961 gctatcgttg attttttcaa caggcataac tcacccagcg gaaaccgctc tacagaggtt
     7021 taaatttctt atgtaccagg ttgttgcgtc tgatttagat ggcacgttac tttctcccga
     7081 ccatacgtta tccccttacg ccaaagaaac tctgaagctg ctcaccgcgc gcggcatcaa
     7141 ctttgtgttt gcgaccggcc gtcaccacgt tgatgtgggg caaattcgcg ataatctgga
     7201 gattaagtct tacatgatta cctccaatgg cgcgcgcgtt cacgatctgg atggcaatct
     7261 gatttttgct cataacctgg atcgcgacat tgccagcgat ctgtttggcg tagtcaacga
     7321 caatccggac atcattacta acgtttatcg cgacgacgaa tggtttatga atcgccatcg
     7381 cccggaagag atgcgctttt tcaaagaagc ggtgttcaat tatgcgctgt atgagccggg
     7441 attgctggag ccggaaggcg tcagcaaagt gttcttcact tgcgattctc atgaaaaact
     7501 tctgccactg gagcaagcga ttaacgctcg ctggggcgat cgcgtcaatg tcagtttctc
     7561 taccttaacc tgcctggaag tcatggctgg cggcgtttca aaaggccatg ccctggaagc
     7621 ggtagcgaag aaactgggct acagcctgca agattgtatt gcctttggcg atggaatgaa
     7681 cgatgccgaa atgctgtcga tggcggggaa aggctgcatt atgggcagcg cgcatcaacg
     7741 gctgaaagat ctgcatccag agctggaagt gattggtact aacgccgaag acgcggtgcc
     7801 gcattatctg cgtaagctct atttatcgta atcgttcttt atttggtcag ttgtcaacct
     7861 gatacttcgc tacaatggat actcgttaat caaagagttt tccattgtgg cgctacttat
     7921 catcaccacg attctgtggg ccttctcctt tagcttttat ggcgagtacc ttgcggggca
     7981 cgtcgatagc tattttgcgg tgctggtgcg cgttggtctg gcggcactcg tttttctgcc
     8041 gtttctgcgc acccgtggca atagcctgaa aacagtcggc ctgtatatgc tggtgggcgc
     8101 gatgcagctc ggtgtgatgt atatgctgag tttccgcgcc tatctctacc tgacggtttc
     8161 tgaactgctg ttgttcaccg tactgacgcc gctctacatc acgctgattt atgacatcat
     8221 gagtaagcgc cgtctgcgct ggggctatgc ttttagcgcc ttgctggcgg tgattggtgc
     8281 cgggattatt cgctatgatc aggtcaccga ccatttctgg actggtttgt tgctggtgca
     8341 gctctccaat atcacttttg ccatcggcat ggtgggttac aaacgtctga tggaaactcg
     8401 cccgatgccg cagcataacg cctttgcgtg gttctatctt ggcgcgttcc tggtggcagt
     8461 gattgcatgg ttcttgctag gaaatacgca gaaaatgccg caaaccacgt tgcaatgggg
     8521 cattctggtg tttcttggcg tggtggcttc cgggattggc tactttatgt ggaactacgg
     8581 cgcgacgcag gtggacgccg gaacgctggg cattatgaat aatatgcacg ttccggcagg
     8641 gctgctggta aacctggcta tctggcacca acagccgcac tggccaacgt ttattacagg
     8701 cgcgctggtg atcctggcct cactgtgggt gcatcgtaag tgggtcgctc cgcgctcttc
     8761 acaaacggca gatgatcgca ggcgtgattg cgcgctgaac gaataaacgc ttctgtaatc
     8821 ggctgacgct gctcgccatc gcgcacggcg gcgtacagtc ggctccacaa gccttcgccc
     8881 agggttttgg tgaccaccag accctggcgt tcaaaactct ccaccaccca atgcggtagt
     8941 gcggcaatac ccatccgtgc ggcaaccatc tgaatcaaca ataaggtgtt atcgacgctt
     9001 ttcagtgacg ggctgacgcc tgccggctga agaaaatgcc gccagacatc cagtcggcta
     9061 cgctgcaccg gataaatcaa cagcgtctcg ctggcgagat cttccggtgt aattcgcgtt
     9121 ttcgccgcca gtggatggtc aggtgctaac accagacgca cttcatagtc gaacatcggc
     9181 gaataatgca ggccactgcg cggcagaata tctgacgtca tcaccagatc cagctcgccc
     9241 tgttgcaacg caggctgcgg atcaaacgtt acgcccgatt taaaatccat ctccacctgc
     9301 ggccagttct tatggaaatt ttctaatgcg ggcgtcagcc actgaataca gctatggcac
     9361 tctatggcaa tgcgcaggcg cgtctgctgc ggttcattgc aggcttgcag tgcctggcta
     9421 atttgcggca gcacctgatt tgccagttgc aacaggattt ctccctgcgg tgtaaagcga
     9481 agcggctggc ttttacgcac aaatagccgg aagccaaggc gttgttccag atcgctaaac
     9541 tggtgagaca gggcggattg cgtttgatgc aacgtcgccg cagcggctgc gagcgagccg
     9601 cagttccgca acgcttgtag cgttttcagg tgttttactt cgatcatgaa agtccttcac
     9661 ttcggcatga ataatttgcg cttgaggaat atacagtaac cgccaattat ggatgtgtaa
     9721 acatctggac ggctaaatac taaaaattcc ataaggggca tataatgaca attcttaatc
     9781 acaccctcgg tttccctcgc gttggcctgc gtcgcgagct gaaaaaagcg caagagagtt
     9841 attgggcggg gaactccacg cgtgaagaac tgctggcggt agggcgtgaa ttgcgtgctc
     9901 gtcactggga tcaacaaaag caagcgggta tcgacctgct gccggtgggc gattttgcct
     9961 ggtacgatca tgtactgacc accagtctgc tgctgggtaa tgttccgcca cgtcatcaga
    10021 acaacgatgg ttcggtagat atcgacaccc tgttccgtat tggtcgtgga cgtgcaccga
    10081 ctggcgaacc tgcggcggca gcggaaatga ccaaatggtt taacaccaac tatcactaca
    10141 tggtgccgga gttcgttaaa ggccaacagt tcaaactgac ctggacgcag ctgctggagg
    10201 aagtggacga ggcgctggcg ctgggccaca aggtgaaacc tgtgctgctg gggccggtta
    10261 cctacctgtg gctgggtaaa gtgaaaggtg aacagtttga tcgcctgagc ctgctgaacg
    10321 acattctgcc ggtttatcag caagtgctgg cagaactggc gaaacgcggc atcgagtggg
    10381 tacagattga tgaacccgcg ttggtactgg aactgccgca ggcgtggctg aacgcataca
    10441 aacccgctta cgacgcgctc cagggacagg tgaaactgct gctgaccacc tattttgaag
    10501 gcgtaacgcc aaatctcgac acgattactg cgctgcctgt tcagggtctg catgtcgatc
    10561 ttgtacatgg taaagatgac gttgctgaac tgcacaagcg tctgccttct gactggctgc
    10621 tgtctgcggg tcttatcaat ggtcgtaacg tctggcgcgc cgatcttacc gagaaatatg
    10681 cgcaaattaa ggacattgtc ggcaaacgcg atttgtgggt ggcatcttcc tgctcgttgc
    10741 tgcacagccc catcgacctg agcgtggaaa cgcgtcttga tgcagaagtg aaaagctggt
    10801 ttgccttcgc cctgcaaaaa tgtcatgaac tggcattgct gcgcgatgcg ctgaacagtg
    10861 gtgatacggc agctctggca gagtggagcg ctccgattca ggcgcgtcgt cactctactc
    10921 gtgtacataa tccggcagta gaaaagcgtc tggcggcgat caccgctcag gacagtcagc
    10981 gtgcgaatgt ctatgaagtg cgtgctgaag cccagcgtgc gcgttttaaa ctgcccgcgt
    11041 ggccgaccac cacgattggt tccttcccgc aaaccacgga gattcgtacc ctgcgtctgg
    11101 atttcaaaaa gggtaatctc gacgccaata actaccgtac gggcattgcg gaacatatca
    11161 agcaggccat tgttgagcag gaacgtttgg gactggatgt gctggtacat ggcgaggccg
    11221 agcgtaatga catggtggaa tactttggcg agcacctcga cgggtttgtc tttacgcaaa
    11281 acggttgggt acagagctac ggttcccgct gcgtgaagcc accgattgtt attggtgacg
    11341 ttagtcgtcc ggcgccgatt accgtggagt gggcaaaata tgcgcaatcc ctgactgata
    11401 aaccggtgaa agggatgttg accggcccgg tgactattct ctgctggtcg ttcccgcgtg
    11461 aagatgtcag ccgtgaaacc atcgccaaac aaattgcgct ggcgctgcgt gatgaagtgg
    11521 cggacctgga agccgctgga attggcatca ttcagattga cgaaccggca ttgcgcgaag
    11581 gtttaccact gcgtcgcagc gactgggatg cctatctcca gtggggcgtg gaggctttcc
    11641 gtatcaacgc cgccgtggcg aaagatgaca cacaaatcca cactcacatg tgttactgcg
    11701 agttcaacga catcatggat tcgattgcgg cgctggacgc agacgtcatc accatcgaaa
    11761 cctcgcgttc cgacatggag ttgctggagt cgtttgaaga gtttgattat ccaaatgaaa
    11821 tcggtcctgg tgtctatgac attcactcgc caaacgtacc gagcgtggaa tggattgaag
    11881 ccttgctgaa gaaagcggca aaacgcattc cggcagagcg tctgtgggtc aacccggact
    11941 gtggcctgaa aacgcgcggc tggccagaaa cccgcgcggc actggcgaac atggtgcagg
    12001 cggcgcagaa tttgcgtcgg gggtaaaatc caaaccgggt ggtaatacca cccggtcttt
    12061 tctcattaca gcggcgtctt cccagcatac tgcgtaaacc attccagata tcagttcata
    12121 ccctgtttaa gaaaatcttc tttattccct tctcatgaaa taaatttcgt catttccgcc
    12181 attttttcta tctgcctctc catcacatat taaaaattac ttgatatctc tatgtttttt
    12241 aatgaaaaac atatcaacct atgatctttc ttgtgactag cttcacattt catactgatg
    12301 cattggaatg aaataaaatt tcatgagatt ttgtgtttga gaaaattatt tcatgaggtg
    12361 tgaaatggac gcaaagctgt acatcactga gatcatcaat gaattaacct tcgttaagga
    12421 ctctatccgt gaggatgact gcaccaaatt gatcgacgct attcagcaaa gccagcgcgt
    12481 tttctgctat ggcctgggac gtgccggctt ttccatgaaa gccttcacca tgcgcctgat
    12541 gcatatggga aaagaggttt acttcctgac ggaaaccatt acgccgaact ttgggcctgg
    12601 cgatctgttt atcgtttctt ccgcttccgg tgagacagca caactggtgg cactggcgaa
    12661 aaaagcacgt cagcttggcg gcgctgtggc ggtgttaacc acgaatcgac acgccactat
    12721 caccgagttt gtcgatgtca tcgtgcaaat taatgcccct tccaaaaatc aaaaagattc
    12781 tgttttccgc tcggcacagc cgatggcgtc gttgtatgag caagctttgc tggtgattgc
    12841 cgatgcatta gtgatgaaga tggctgctga aagtggtgcg ccggaaagtg aactgtttaa
    12901 gcgccacgcc aatctggaat agtcgtcgcc gttatttgct cgatacccta caggagaata
    12961 aaaatgagca aaaaaataac atccttgttt aacttcaaga agttgcagaa attttcttct
    13021 gctctgatgt taccaatcgc cattcttccg gcggcgggtt tgattttagc catcgggatt
    13081 tcatttaaca ttgagctgct ggcgaattgc ggcggcctga ttttcgccaa ccttcctttg
    13141 ctgttttgtg tcggtattgt gattggcctg acaggtgatg gttcttcatc actttcagcg
    13201 attatcaatt acatgatcat gaatgccacg atgggaacgc aacttggcat taccgcggag
    13261 tccgttgcag ccagtaacgg tgactatgcg atgatcctcg gggtaccgac gttacaaacg
    13321 ggagtgtttg gcggtttgat tgtcgccatc tttgtttatc tgctctatac ccgcttccac
    13381 aatattgaat tgcctcagtt tcttggtttc ttcggtggaa aacgctttgt acctattatt
    13441 acggcggtgg cggcaattat tgtcggctat ttattgccgt ggctgtggat aacggtacaa
    13501 aaaggtctgg cgtcgctttc aacgctggtg accagtgggc ataacgcgaa cttcgcggcc
    13561 tttatttacg ccgttggcga acgttcgctg attccttttg gcctgcatca tatctggaac
    13621 gtgccgtttt attacaactt tggcgactac atgaccaaat ccgggcaact ggtgacgggc
    13681 gatattccgg tgttctttgc ccaattgcgc gacggtgttc cgttgacggc ggggctgttt
    13741 atgacaggtc gtttcccgat catgatgttt gcgttacctg ccgccgcgtt ggcgatgtat
    13801 caggaagcaa aaccggaacg taaggcgctg attaaaggcc ttttactctc cggtgcgctg
    13861 accacatttg ttaccgggat tacggagccg ctggaatatg catttttgtt tgcagctccg
    13921 gttctctaca tcattcacat cttcttatat gccacttcat ttgtgctgat gaacatcctg
    13981 aatgtgcaca ttggtcatcc ttttgctggc ggtcttattg atttcgtact gaatggcgtt
    14041 atgccaaacc gcacgccgtg gtatctggtg ttcctggcag gcgcgggcta tgcagcgatt
    14101 tactacaccg tatttcgggt actgatccgc aagttgaatc tgaaaacacc gggacgtgag
    14161 gatgaagaga ttgccgttgc aaccacatta acgcgcgaag aacgtccata ccaaatcatc
    14221 gaagcagtag gtggtttcga caatatcgaa gatgtggatg cctgcgcaac acgcctgcgg
    14281 ctggcgctgg tagacgataa gaaagtgaat gaaaaacggc tgaaggagtt gggggctgcg
    14341 ggtttagtga aactgggtga tggcggtgta caggtgatat ttggcggcaa atcgcaaatc
    14401 ctgcgcgacg aaatcaaaac cgtaatgagc agaccgcgcc cgacagaaat gacagtggcc
    14461 tgtgctaact aattcgagat tagcgggctg gtttcagccc gccagacaga gagagctgaa
    14521 gtatgaattc acaattactg gctaatgcca tccgaatgtt aagtgttgat gcgatccaga
    14581 aagcgaactc cggtcatccg ggcgcgccga tggggatggc ggatattgcc gaagtggtct
    14641 ggcgtcgtca cctgcgtcat aacccaaaaa atccgcagtg gtttaaccgc gaccgctatg
    14701 ttcagtcgaa cggtcacggc tccatgttga tttacgcttt gctgcatctt acaggctacg
    14761 acctgtcgat ggatgatatt cgcgactttc gtcagctcca ctcccgcacg ccgggtcatc
    14821 cagaatatgg ttatacgccg ggtgttgaaa ccaccaccgg cccgttgggg cagggcgtag
    14881 ctaatgcggt ggggatggcg attgcggaga aggcgctggc ggcagaattt aacaaaccgg
    14941 gctttaatat tgttgatcat cacacatggc tatttttggg tgatggctgc ctgatggaag
    15001 ggatctccca cgaggcctgt ggtcttgcag gaacgctgaa gctcggcaat ctgattgcta
    15061 tctgggacga taacggtatt tcgatcgatg gtcacgtaga aggctggttc gctgaagata
    15121 cggctgcccg ttttcgtgcc tacggctggc atgtcattga aggcgttgat ggtcacgatc
    15181 cagaagcggt agatgctgcg gtgcgtgaag cgaaaagcgt cacggataag cccagtctgc
    15241 tgtgctgcaa aacgattatc ggttttggtt cgccaaataa agccaacagc cacgattgcc
    15301 acggctcggc gctgggtgca gatgaagtgg cgctggtgcg tgaacgtctg caatggccgt
    15361 atgccccttt cgaaattcct ggtgaaattt atgccgaatg ggatgcgacc gagaaaggcg
    15421 cgcaggttca acaggaatgg gatgcactgt ttgccgacta cgcgaagcag tggccggaac
    15481 tggcggcgga gtttactcgt cgcatgaagg gtgatttgcc tgctggctgg gccgagaata
    15541 tgcagaaata tgtgcacgat ctgcaatccc atccggcggc gctggcaacc cgtcaggtca
    15601 gccagaaatg cctcaatcac ttcgcggata tgctgccgga actgatgggc ggctcggcgg
    15661 acttgtcgcc ctctaacctg actcgccatc agaaatcggt tgatttcacc ggggaaaatc
    15721 cggcggggaa ttacatctcc tatggcgtgc gtgaatttgg tatgtcggcc atcatgaacg
    15781 gtcttgcgct gcatggtggg tttatccctt acggcggtac tttcctgatg tttatggaat
    15841 atgcgcgtaa tgcactgcgc atggcggcgc tgatgaagat ccgctcggtg tttgtttata
    15901 cccacgatac catcggcctg ggcgaagacg gcccgacaca tcagcccgtt gagcaactgg
    15961 cgtcgctgcg tctgacgccg aatatggaaa cctggcgcgg ttgcgatcag gtggaagtgg
    16021 cggtggcctg gcaacaggcg attgagcgca aagatggccc aacgtcgttg gtattaacgc
    16081 gccaacctct ggcgcaacag ccgcgcaccg ctgcacagtt ggcagagatt gcgcgtggtg
    16141 gttatgtact gagtgattgt gacggtcagc ctgagatgat cctcatttct gcgggttcag
    16201 agattgagtt agtggtcagc gccgcaaaag cgttaacgga agaagggcgt aaagttcgcg
    16261 tggtttccct gccgtgtacc gagcgtttcg ataatcagga tgcggcgtac aaagagtcgg
    16321 tattgccaaa agctgtacgc aaacgtctgg cggtagaagc cagcatcgcc ggattctggg
    16381 aacgctacgt gggcctggac ggtaaagtga tcggcatgac gagctttggt gagtctgctc
    16441 cggcgaatgt gttgttcaaa cactttggtt ttacgccgga aaatgtcctg gcgcaggcgc
    16501 gtgagctact gaacagctaa acctgcacaa ggggtgaaag gatgaccttt taccccttga
    16561 aacgggagcc gagaaactct tcacgggtgc ggtatttctc gtaatgcgcc ttgtttttat
    16621 acataaacaa cagcaccagc atatcaataa cgcccattgc cggaacgcga gtggagtcga
    16681 aggcttcaaa gaagatttta tcgctgaccg cggtattgag ttgatgggtg gagagttttg
    16741 ataacggaga tttaatatag ttagtgatac aaacggattt ggcaccgcgc tctttcacca
    16801 ctttaaagat atcgaccatt aagttggtgc tgccagagtt agaaatacca atcgccaggt
    16861 cgcgtttccc catagaatat gccgcattaa tttgcatttc cgggctgacg tgaacaacgg
    16921 tttttaatcc gagttggctg aatttataag caccataaac ggccagaggg tttgaatttc
    16981 cggcagcaaa aaagaaaata gtatcgcaat tagcaagtag tttgagaatg gactcaagca
    17041 cttcggcatt aaaaaattgc gttgattgta ttaaatcttc ggcctgtttt tttaataaaa
    17101 atgtggccag cgacgttata tcggtaaaat cggtattatc aggatagcta ttgtcaggat
    17161 taatgacttc ttttgctaat gcaattttta aatggtaata accctgataa cctaatttct
    17221 tacaggtgcg tacgatagtc gcttcactgg catggctttt ttctgatagt tcagtgacac
    17281 tatagttcac gacttcatgt gcgtggctaa gaatatagtc agcgaccttt ttttccgcct
    17341 gatgcagtgt agggtaattg ctttgaatca gactttttac ggttatgtta ttcataatta
    17401 gtaaatttca ttctgacaga aagtgctgat tataacatag ttttcaggcg tggataggtg
    17461 tgaaaaaaat gaaggtgtgc agtgaacgta atgattaaca gaccggttaa ttttcatgaa
    17521 ttacgtcaac tgcacaaaaa taatttaaac gggcgctcta attaaagcaa gacttatatt
    17581 tttatcattg tattgtcaat taaatacaaa tcgtgtcatt aattgtgggt ttcttaaata
    17641 ttctatgcac cactgacagg ctttgcccat accatcggaa cgagcggcaa gaaataactt
    17701 ggtggcgtgt ttatgttcgc gcattggctt tttaagcaat ttaccgctgt tcaataacgg
    17761 caatgccaga tgatgtggaa tataaccaat accgacgttt tgttctatta aggcgattgc
    17821 cgtagcgaag tcaggaacaa aaatgggttt ttgtccttcc aacaaccagg cttgcatcgg
    17881 tgggaagtta acggaggtat cgcggataca aaccgctgga taatgacgca attcactgtt
    17941 ttctaatgga tgccgcttcg ctgccagtgg gtggagtggg ctgacgacaa aatcccattc
    18001 catttgtccg accggttcgc taatgatgcc ttcactgctt ggtactgcat gaggagcacc
    18061 gtagaccaga tcagcacgct tactgtacag cgcatcccag cagccgttgt atacctcaag
    18121 atctaccgtc agtgttgttg atgtgaattg ccgttcaaaa tctcgaataa atgccaccag
    18181 caaatcacca ggaataatat tgtttaccgc aattgtcagt tcacgctcaa taccatcgtg
    18241 aatcagcact gtgttgcgct ttaatgcttc caggtcgcca agaatagttt tgctatgttc
    18301 gatgaagtaa cttcccgctg gcgataattc aatatagcgt cccttacgaa tgaacagttc
    18361 aacgcccagg ctctcttccg ctttcttgat ggtgtaactg atagcggaag ggactttatt
    18421 taactgctcg gctgcggtgg taatgctctg gtgtctggcg accagatgaa taacacgaag
    18481 cgtttcgtct gagatgaaca taaggctccc tggtcagtgg taaagtggct gaatctctcc
    18541 agattcagcc gtaatcattg cctgagtata cccaggaatt agcggtgttg acttgcccga
    18601 ctgagaatcg catacgcgat acatgccacc agaatcgagt ctaccgaagg gatcgtcgtc
    18661 agggtgaata agccttttac ggtcataaag ccaaccgctg cgcctatgat ccatgctgcg
    18721 aatgtcagca ctttaatctg tgactcctgg gctaacactg actcttgata gccattacgg
    18781 cgataaagga aaaagtcagc gatataaatt ccggcaacgg gtggcgtggc gatacccagg
    18841 aacagcagaa acggaataaa ccacgccata atatccatac tgcctacgat ggcggaaagg
    18901 atacccagcg ccacggtaat ctgccatttg ggaaagcggg taagcagtgt ggaaaccacc
    18961 agcgtgccct ggaacatatt gcccgcgtta ccggtgacgc aggcgaaaat caacaacagc
    19021 gccgcaggca gaaccgcacc gaataccgcc atcgcaccca gtaaagatcc ctgaccacta
    19081 atggcgctcg gcgttgcacc cgcccagtag agcagagggt aggcaaccag aaacgtcacg
    19141 cctgcggcaa tcagcgcatg tttgcggtta tgcacaaaac taccgaaatc aggcagtgtg
    19201 gcaaccagca caataatggt gccaacaacg gtggaaacgg cgacacctgt attcatctcc
    19261 acaatcgccg ctggtactgc cacttcggta tgggttgcca catagaggat gtaacacagc
    19321 accagcgcga taaccggcac ggcaacctgg gcaatttttc ccagaacggc aaagccaaat
    19381 gccgtagagg cgacaaaaat gacacagcca gccgccacta acagtgccag tggaacttcc
    19441 agtccatgtt gagccagtaa gtcatgtacc gaatgaccaa acatattggc ggtaacggca
    19501 atccagccga acagacttat cgccatcaaa atgttaatgg cgatggcccc tttttcgcca
    19561 aacgaatatt tcacaatgcc atacgttggc aggcgggctt tttctcccac gctaatggtg
    19621 atggctgaaa gtaaggtcag aatgacgccg cctaacaata cgacgctaat taaactgccg
    19681 ctggaaagtt gattgccgag gcttgaggat gacaacagca caggtgtgac ggcaataccc
    19741 agcaaaatca tggcaatacg caatcctgat gccgtattca gattctcttc ttttttacgc
    19801 atagtatgtt ccctttataa atgcgcgtta cacagtatga aaatgcccgg tgtatttgcg
    19861 ccagctgccc agttctgtaa tttcccgttg gccttcggtc agccagtttt ctgccagtac
    19921 gcccagcact tcgctaccat cggcaagttt gattttgcca attgccagcc cagcgggttc
    19981 gctcattaac agagcggcaa aactggccag cggtaattgc caaatttcta cgtcgacgtg
    20041 tgttcctcct tcattcacgc gaatcattcc cggatggcga tcattaatac tccacaaccg
    20101 gtaatgggcg tcagttcggt cttcacggac aaaaataccg cccgcttttt gcatatttgg
    20161 gttgagttcc aggccgcgca tcagcgttcc gttaacggct aaaagcgttg tagtcattgt
    20221 gttctccaga gaattaaagg gttatccatg taccgctctg atgcgccagg gctgctttta
    20281 tggtttgcgg tgaagtaatc aggccgctgg cctgtttgcc ttgttgctgg ctggcattga
    20341 tgaaatcaac aatggcttcc actttgggtt gcatactgcc gacaccaaac tgaccttccc
    20401 gcaacagcgt ttgcgcttcg gcaatgctga tagcgtcgag ccactgttgt tgtggtgtac
    20461 caaagttaat cgccactttt tctacaccgg tggggatcac cagcagatct gcgcctaact
    20521 gactggcgag cagcgcggag gccagatctt tatcgatcac ggcttctaca cctttcagtt
    20581 gttgcccgtc tcgcaccacc ggaataccgc cgccgccgca ggcaataacc agatatccct
    20641 ggcgcaccag gtgagcgatg gtgtcgtgct caataatttc cagtggtgca ggagagggaa
    20701 ctgtacgccg ccaaccacgc ccggcgtcct ccatcagcgt ccagccgagt tgttgttggc
    20761 gttgttgggc cgttgcttca tcgagaaacg cgccaattgg tttactgggg ctggcgaaag
    20821 catcgtcatg tgggctaact cgcgtttgtg tcaccagggc aattaccggt ttgtttatgc
    20881 cacggcgagc caattcgtta tgcagcgctt tctggaacat gtagccaatt gcaccttgtg
    20941 tatcacccac ggcgtaatca agtggaaccg gagaaacttc gttactggcg agttcagagc
    21001 ggcgcagaat aaagcccacc tgcgggccat taccgtgggt aagtaccagg tcccatccgg
    21061 cttcaaccat atcaacgata tgttgcacgc tttccatcac tgcaacatat tgatcgggaa
    21121 tactattgcg ttgctcgtcc tgaatgagcg cattgccgcc aactgcgaca acagccattg
    21181 gcttaaccat gatggttctc ctttgcgacg atctccgcca gtgcacgtac agcggcgaca
    21241 aaacaggcca tcggcgcagt ggtgatccca gcaccaattt gcccgacccc cgcttgtttg
    21301 tgggcgatac ccgtattgat gaccggtaaa atgccgcgat cggtcacttt acgcgcatca
    21361 atccctgctg cggtgggggc aaaattgagg gctggaagtg taaatgcggg gttaccgccg
    21421 agggtgatgg cttgcatccg acggctgtta ttggtggcat cggcaggggt gcctcccaca
    21481 aatttgacga tcgccggaga cgacgccatc gcgaagccac ccacacctgc ggtttcagta
    21541 attgcgctat cacccaggtc agcagcagca tcgtcaacgc caaatccggg gaagaacaga
    21601 ccttcaacgg cattggcagg agcctggaac cagcgatcgc cagtaccgga aagccgaata
    21661 ccaaaattca ccccgttacg cgccatcacc gtaaccattg aactgaacgg tacattggcg
    21721 gcggcatcca tcattgcttt acaggcagcc atcgacagat tgaggaagaa gtgatcgtta
    21781 ccggtgataa atgccacgac gcgctggata tgttcctgtg gaagagagca ggccagcaat
    21841 gccgggagca ggcgcttaat cagcaaaccg gtagcggcag cattgcggtt atgaacttca
    21901 tcccccatat gcagcgcctg cgccattaat ggcttgagtt ccagctcgcc gtgttgtgcg
    21961 attgctgctt tcattgccgg cgccagttca tcgcgcatcc aggccagacg attgagaact
    22021 tcatcgttat tggcgccaaa acggagaacc ttacccagac cttcattgaa attgctaaaa
    22081 gtacggtggc cgttggtttt gttttctacc acccataacg gcatcgacgg gctgatgatg
    22141 ccagccattg ggccaaccgc gtgataatga tgacaagggg caaggtcgat ttcgcctgcg
    22201 tttatctggt tttcagcgtc ttgctggctg gtggcccaac cttcgtacag catggcacca
    22261 ataatcgccc cttttaccgg accacacatc tcttcccagg caatcggcgg gccagagtgc
    22321 aaaatacgtt tttgttcagc cattgctgga atggcctctt tagcgagcat gacatccacc
    22381 agcatgggct gtgcttcgag ataacgtgtc atggcgattt ggttggcatt ttctaccagg
    22441 ggatgacgta gcaacgatgc cagatccaat ccagcgtcaa tatcgccttg tgctggtggc
    22501 tgccagttga gtgcaattgc gcttcccccg gcatgttgaa tattgtcagc aaaactgtgt
    22561 agtcccgcgt tcacaacctt caaaggctga ttaaataagg tattcatgcg tttagcccct
    22621 ttttctgcaa ctgagtctgt gcaacggtgc tggcccatag cgccgcctgt gcattgctgc
    22681 tggcgatgat gacccctgca ttttgtagtg ctcttatctg ctgactgcgt atttgcggat
    22741 cagcttccgt tccgcaaacg tgcgcaatca gcaacggtgc gttattcatg tcgatgtgtg
    22801 aaagttggtc cagtaattcg ctggcaggcg tcgtggaagc gccataacca agtaccaggt
    22861 cgaataacac gacggcagtg tgtgagtcgt tgagttcgtt gagcagacgt tgattgcgca
    22921 gagtcgggtc gatcattgga tgcggtttgc cacgggtgaa atcatcgtcg cccatatcga
    22981 tcaacgtgtg atcttcgctt tgccagatat ttgccagagc acgattaccc gcaactggcg
    23041 tattggaagc tgcgctgaat cccttttgtt ggcagataag ttgcgcttcg tagcagaagg
    23101 ttcctccggc gaaaacaccg cgtatcgcct ggcgatgggc tggcaggcgc tggcaggcgt
    23161 tttgtaacat tgtgagatcg tcgcagggta catcggtttc gacagccgga agtggctgaa
    23221 cattcagtaa cgccacagcg atgtcggcgg cgctggcaag tgtggtggcc gcggtgatat
    23281 tgggacgtgc cagatcatgc ggattcgccc cgaggaaatt caccactacg ggttttccac
    23341 aagcctcggc acgctccaga atagtccggg caacgatcgg tgaaggcggt tttgaaataa
    23401 gcacaattac gcgagtttca tcgtcttgcg ccagcgcatc taaagcgaag agcatcgata
    23461 ttccaccaat ctcttcactg agatcatgtc cgcctgttcc caacgcctga gaaataccca
    23521 ctccgagctg gtcgattcgg caagtcactt cctgtagccc ggtgcctgac gcgccaataa
    23581 caccaatcgc tccacgtttc accacattgg cgaagcctaa cggaatgccg ttaacaatgg
    23641 cggttccaca gtcaggtccc attacgatgc gctgacgttc tcgggcaagt gttttgatgg
    23701 atttttcctg cgcgatactg acgttatcgc tgaacatcat cacgttcatt ccgaggttta
    23761 acgctttaat tgcttcggcg gcggcgtagt ctcccggtac agaaatgagt gccaggttgg
    23821 cttcgggttc ggcttccagt gccatttcca ggctgacctt ttccggtgtt ttaatgccac
    23881 tacccgtttc atcatcgggt ttgctggtca gacgttgctg ggccagtatc agtgcttcat
    23941 tgcaaatatc ttcttcaccc atgactgcaa tcactaaatc attgggactg gcgttgattt
    24001 cgttacccag acccgcatcg cgcaattgtt cgagattatt gggcgttccc atgacgaccg
    24061 aggcctgttg aataccgggc aacttgctga tttgcgcgga aatctgcatc agagaaactg
    24121 agtcctgata aagattactg aaaactttat atttaacttt catgctgtta cgcctgtttc
    24181 attccgcccg atagcaaaat tcgggcaaag catccccgtg tagccccata aacgggctac
    24241 gagagaaaag gttgaaatca attcatggtg ttcagggttc gaaagccatg cagcattcct
    24301 gcaaggcaat ctactcctga cgttgcgcca aattgcatga cctgttctgc acaggatgcg
    24361 attgtcattg ccgatgcgct cccaaccagt tgtgcgagta actggcaaat cggttctgaa
    24421 aaatgtccct gaagcgcacg ctccagataa tgacgactga tatccgtggt gttgtgcgca
    24481 tgttgatcaa ttgcctgctg caaccggtat tgatgatcgg caagtggtgc agggagttgc
    24541 cataaccaga gtgctgccag atagcccaac aaatagtcat ctccatcggg cgttaagcct
    24601 ttaccaaagc cgatcagttg tggcacctgg cgttccagcg catctggatg ttccataatc
    24661 gctaatccct ggtgcgtgag actatccggc aggtgcaatg tgctttgaac gttatgttcc
    24721 agacaaaata aaagccgttg cgatgccatc gttgggtagt gctggaaaac caaagggaat
    24781 gtctcaagtg tcagcattcc ccccacaaat ggcgactgcc agagcgttgc gttatcgagc
    24841 gccacacaaa atcggggtgt ccgcagtaag cctggggtta actgaatggg atctgaatgg
    24901 gcaatctctc ttcgccagtc ccaacattca ggtagccaga ctctggcagc atcagggaga
    24961 ttcagatacg tttgcgcaca caacagagtg atgaatgttc ccgcgtctga atacagattt
    25021 accgcatgtt taaaagcgct atgcagacgt aaatcttgcg tttcattctg cgggaaaaga
    25081 tatcccaacg acgatgcgca aagcgtaacc tgtaccatcg gcgttagcct tccagacctg
    25141 caataatggc tttggaatca gtaacccaac cgaaaatggc accttgtgct ttgatcatct
    25201 cgagggcgta tttctgaaat tccgggaaat aggagccgac acaatcttcc gggataatac
    25261 attcatagcc acgatcattt gcttcgcgca cggtggtggt tacgcaaact tcggttgtta
    25321 caccacaaac tatcagcgtc ttgataccgt ggttttgcaa aatcagatgt aagtcggttt
    25381 gataaaaagc gcctttgcca ggcttatcaa tcaccggttc gcctgccacc gggtacagtt
    25441 caggaatgat gtcatgacct gcttcacctc tgaccagaat gcgtcccatc ggtcctggtt
    25501 caccaataaa ggtttttccg ccgcgcgtca gtttggctgg cgggcagtcg cttaagtctg
    25561 cacgatggcc ttcacgagta tgaataacca taattccttt ttgacgtgcg gctgccagca
    25621 cctctgtaca tggtgcgata gccgtgcgta cccgtgaaac atcattgccc agtgcctcac
    25681 cgaagccccc tgcttcgaca aagtcacgct gcatgtcgat catgaccagt gcagttgtgc
    25741 gcggatcaaa aggtaattca aagggttgag cctgaaaaat ggattgcgtc atggtaaatc
    25801 tcctgtctga tgtttaaacg atccagttgt tctgagaacg agtattcagg agaaaagcgg
    25861 cacaaaacag gaaaaaagtt tgccgcacag caacgaaatt tttgcattca gcaggtggtg
    25921 tgatgacgaa tgtgaaggga gtcaaagagt ggcatacagc aacaaatttt ttgatgctgt
    25981 atggttttga gattaaaaaa atcgttgaaa ttgtgtgagc ttaatcatca gatttagcga
    26041 ctatggcgtt actttttaac accatactgt gtaaaccatt ccagcatacg ctgccagcca
    26101 tctttcgcag attcggcatg atagctcggg cgataatcgg cgttgaatgc atgcccggca
    26161 tccgggtaga cgataatctc tgctttcgca ttagcagccc gcagcgcctg gcgcatggtt
    26221 tcaacgctct cctgcggaat gctgttatcc tgaccaccat ataagccgag aaccggcgcg
    26281 ttaagatcgg ttgcgatatc aacaggttgt ttcggtgaat tcagcgactt atcgcccgtc
    26341 agtttgccgt accacgccac tgcggctttt agctgtggat tatgcgcggc atacagccag
    26401 gtgatacgtc caccccagca gaatccggtg atcattaaac gatgaacatc gccgccgttg
    26461 cgggaagccc aactggcgac atgatcgaga tcggccagca cctgcgagtc aggcactttt
    26521 gctaccagac cgctaagcaa cgtggggata tcggcaaaat cattcggatc gccttcgcgg
    26581 aagtaaagtt caggtgcaat agccagatac ccctccagcg ccagacggcg acaaatgtcg
    26641 cggatatgtt catgcacgcc aaaaatttcc tgcactacaa tgaccactgg cagtgggcca
    26701 tcgctttgct ttggtctggc atggtaagca ggcatgttat ccccttgtga agggatagag
    26761 gtgaagcccg ccacaatcgc gtcgtccgga gtctgaacga tggtcgaagc gagaggcgat
    26821 gcagcaggtg caaatccaga ttgttgtgtt gttgccatgg tattctccgt acccttataa
    26881 aaatgttgcg caatgttaac tatagtcagc atgcaacaaa tcacattgcc tgaatcggct
    26941 catcttttat gcagtcctgc agaatgaaga gtgatttatg tgatttgcat cacttttgat
    27001 gggtaaattt atgcaacgca tttgcatcat ggtgatgagt atcacgaaaa aatgttaaac
    27061 ccttcggtaa agtgtctttt tgcttcttct gactaaaccg attcacagag gagttgtata
    27121 tgtccaagtc tgatgttttt catctcggcc tcactaaaaa cgatttacaa ggggctacgc
    27181 ttgccatcgt ccctggcgac ccggatcgtg tggaaaagat cgccgcgctg atggataagc
    27241 cggttaagct ggcatctcac cgcgaattca ccacctggcg tgcagagctg gatggtaaac
    27301 ctgttatcgt ctgctctacc ggtatcggcg gcccgtctac ctctattgct gttgaagagc
    27361 tggcacagct gggcattcgc accttcctgc gtatcggtac aacgggcgct attcagccgc
    27421 atattaatgt gggcgatgtt ctggttacca cggcgtctgt tcgtctggat ggcgcgagct
    27481 tgcacttcgc accgctggaa ttcccggctg tcgctgattt cgaatgtacg actgcgctgg
    27541 ttgaagctgc gaaatccatt ggcgcgacga ctcacgttgg cgtgacagct tcttctgata
    27601 ccttctaccc aggtcaggaa cgttacgata cttactctgg tcgcgtagtt cgtcacttta
    27661 aaggttctat ggaagagtgg caggcgatgg gcgtaatgaa ctatgaaatg gaatctgcaa
    27721 ccctgctgac catgtgtgca agtcagggcc tgcgtgccgg tatggtagcg ggggttatcg
    27781 ttaaccgcac ccagcaagag atcccgaatg ctgagacgat gaaacaaacc gagagccatg
    27841 cggtgaaaat cgtggtggaa gcggcgcgtc gtctgctgaa ataaaaaacg ctccccaact
    27901 tcggggagcg atatcagtta ctgacgccgt aaacgcatta ttttgcggat tattggcgtc
    27961 agtagcgtaa aggctgccag agttagcaat gccagagtga tcggtcgggt atacagaaag
    28021 tccagtgaac cccctgaaag cgacagcgca cggcgaaaat tactttctgc catcgggccc
    28081 ataatcaatg ccagcactac gggtgatgcc ggatatcctc ctttctgcat gaaatagccc
    28141 accacgcctg caatcagcat aatgccaaca tcaaacaggc tattgttcaa cgcataagtc
    28201 ccgaccacac aaagtgcgag gatcatcggt gtgagaatat tgccggggat cttcaccacg
    28261 ttaccgatga ctttcaaaga gagcagtcca agaactagca gcatgaagta acaaaagatc
    28321 atgccgacaa acagtgtata aaccatatcg ccgtggtcgg taaacatcag cgggcctggt
    28381 tgcattccct gtaacgttaa cgcgcccagc atgatggcgg ttaccgcatc gccagggatc
    28441 cccagcgtca gcataggtaa caatgcgcca ccggtgcagc cattcgcgcc tgcctcacag
    28501 gaggccacag ctttaatttc acccttacca aagttttctg gtgtcttact gaaacgacga
    28561 gtttcgttat aggcaacaaa agctgcaata tccgcccccg caccggggat catgccgata
    28621 aaaaccccta atcctgatga gcgcaaaatg gttaatgcaa tgcggcgtag tgtttgccag
    28681 ggcagtaaca gactgccgat agccaccttc gccccctgac gaatttgctg gttttgctcc
    28741 atagagcgga atgcttctga agcagcaaat aaaccaatca tcaccgggat aaatggcaca
    28801 ttcatcagct ccacaaatcc acctgtaaac cgtggaaaac cgcccattgg atcaaggcct
    28861 atcgttgcga tcagcaatcc gccaaccccg gcaatcagcc ctttaatcag cgactcaccg
    28921 gaaatactgg caataatact cagtccgaag gttgccaggg cgaaggactc cgaggcacta
    28981 aatttcagcg cccaactggc aagtaccgga gagagaaaca tcagcacgat aatgctgagc
    29041 gttccgccac taaaggaaga gaacgtggcg gcactcaatg ccagccctgc ttttccctgt
    29101 ttcgtcagct cataaccatc aatagcggtg gctgcggcgg caggcgttcc gggaattttg
    29161 agcaaaattg cggtgataga accaccgtag acgccaccaa aaaagacgcc gcaaatcatc
    29221 aacaatccag aaacaggctc catgccgtag gtgaaaggca gcaaaatcgc cacacccatg
    29281 gtcgctgtca gaccaggcaa agcgccaatc acaataccca gcatcacccc aaaagtggca
    29341 aacagcagcg cctgaggatt attgagtaat gtcgaaaaac cctgagttaa aagttcactt
    29401 tcaaacattg ctggcccctg ttattcgaaa aatgtgccga caggcaatgg cacctgtaac
    29461 gcttcgacaa acagcaacca gaggattgcc gttaacagca gcgcaatacc cggtgtgagt
    29521 acccatcgcc tgtagcccat caacgtcata atgacgatga ggaaaacagg agtagtaatg
    29581 agataaccgc agtagctcat cgccacaata taaaacgcgg ttgccgcaat tcctgttgct
    29641 gttttcctga atgccggttt ttcctgtgcg ttggcgcaag aggctggttt tgcctgcaaa
    29701 agatcgcgta tcaccagcaa gacagcgagc acaatcaggg cacaggcata gaaggtaggg
    29761 aaggcacttg caccgggatc cgtttcaatg gcagttgctg gaaaatcagc ggcattaatc
    29821 atcaccgcgc cagcaaccac acaaagtaac agcgccacca ccatcgttga tgaaaggcgc
    29881 ttgcggtgcg gtacggcgag ttgagcaaca gactgttttg tgttcatcaa ccgtacctca
    29941 ggtttatttt ttcaggccca ggcgagtcag caactcgtca aagtactttt cctgttcgct
    30001 gatttgggtc tggaagctgg cagcgtcaag ccatgcatag ttgagattca acttttgcag
    30061 tgcatcctgg aatgcaggct cttcagccgt ctcttttgct gcctttgcca gaacatccac
    30121 cacatcctgc ggcgtttttt gcgacacaat caggccgcgc caggtgccga tggagagatc
    30181 aacgcctttc tcttttaacg tcgggacgtc aggcatggtt ttcattcgct catccgccat
    30241 cactaccagt gtcttcagct tgccgccatt cacatggttg ataacttctc ctgggcttac
    30301 ggaaaccgct tcaatatgcc cgcctaacag gcctgtaatg gcaggggctg cgccgtcata
    30361 cgggacatga gaaaactttg tgcccgtttt gtcttccagt gcagctgccg ccagatgcca
    30421 gatagctccg gtgcctgagt taccaatgcg tacttttccg ggattcgctt tggagtaagc
    30481 cataaattct tcatagctat tccacggcgc atcggcacgg actgtgatag cagccggatc
    30541 cgcattcaga cgggcaatgg gtttaaagtc gctggtttta aaacgcacca ttccgaggct
    30601 gggaagggtg gtcagttcaa ccgtgcctaa accaattttg taaccgttag ggcgggcagc
    30661 ggcgatttca ctcaggccga tagcaccgcc tccgccaggt ttattgataa ccccgatgct
    30721 gacgggtaaa tggtttttgg cggcatcagc aaaggcacgg gcaaccagat ccgtaccgcc
    30781 tccggcagcg tagggaacga ctaactcgat ctgtttggtt gggtaggagg cagcctgtac
    30841 aggcgcaatg ctgagggcag cgcctgcaag aagtaccatt gcagccattt ttttcggtaa
    30901 ggtaagatgt tttatcattg cttttatatc cttatttatc agttagttgc tatttaaatt
    30961 gttccatgcg ttcataaaca gtactgcatt ttcatagact tgctctggcg tcatgccggg
    31021 gcgatatagc gcagaaccga ggccaaagcc gtttgcgccg tggctcagat aacgcgccat
    31081 ggtttccggt gtgataccgc caaccggtaa catcggaacg gattgtggaa taaccgcacg
    31141 ccaggctttg gtaacttctg gcgtaatttg ttcagccggg aacagcttga tggcattagc
    31201 accatgttca atggcagcaa aggcttcggt gggcgtggcg acgccaggcg cacaatacat
    31261 acccagcgca cgggcgcgac ggatcaccgc agtatcgcaa tgcggcatca cgataatctg
    31321 gcccccagcc tctttaaccg caaccacctg ctctaccgaa agcacagttc cggctccgac
    31381 caggcagtcc ttaggcaggg cgtcgcgcag taaactgata ctttgcaacg cctccggcga
    31441 gttcatagga atctcaatca ggcggaatcc ggcggcgtac agtttttcac cgatggcgac
    31501 aatctcatcc ggtttcacgc cgcgtaaaat ggcgactaac ccggtttttt gcaaaagtgt
    31561 attaaatggc atgggtacat tccctggcgt tggtggacag taacccggca gcgtgggcga
    31621 ttcgccataa tccatgttca gtggcattag gaatggcgtg tatctgtctg tcgggaaaaa
    31681 cgtggctgaa tgcttgtgaa tagcggctgt tcagatttgc gctaccgatt aaggcgatcg
    31741 gtgttgtggc gggtaaatca cgtagcaaac tggatgccag accacataat tcatggccta
    31801 tcagtaatcc ggaaaggtaa tccccctgcg aggacgacgt aagattactg gtcagcaggc
    31861 gcgaacgggt cgaaaacagc gtcgataata atccggtctg atgatgctct tgtgctgctg
    31921 tcagcccgtg ggtgaaggca tcccagtgcg gttcatctcc aggatgcatg gtggcaccga
    31981 gaatcgaatg gcgggataaa acatcaaata actcgcccgt cataaaggtt cggaaatcgg
    32041 taatggtgtt gttttcaact actgcccatt tcgcatgtgt gccgggtaag cctatcagca
    32101 ctggcatacc ctgatggatc gcgttttgca gggccggttc catcgagata gcaccgaaga
    32161 tttgtgtctc ttcgccacgc atcacttcgg gcatttcacc ttctttaatc acgccaggaa
    32221 tgatgtgcag cattgtctgc tgctggtgga taacaggcgt caactgcttc gccagaccct
    32281 cgagagtgac cgggcaaggt aaataagctg ctgattgcca cccttgcgca ctgccgagca
    32341 tgccgcaagc cagtaccggg catggggttt gtgccagcca gtcgccgcag acacgtaaaa
    32401 acgtatcgtg gaaagcgtcc tcacgtgttg ctgcctggga gggtaatttc atgatccccc
    32461 aggggaattc acgtttttct tgtggatttg gtgattcgcc aaaacgccat gcccgaagcg
    32521 atgacgttcc ccagtccagg gcaataaatg agggtgctcc cggcattagt cgtttccttt
    32581 aatcatggtt tatttatctt tgcggacgtg cttaccccag cccagttcag cggatatctc
    32641 ttctgcacag gatttgacgt aaggtgccag ttcttccaga cgcgccagcg acatatacgt
    32701 ggtggtactg gcgacagaaa tagccgccac aatttcatcc cgggcgttgt aaaccggcgc
    32761 tgcaacgcaa cggatagtcg gctcgttctc ttccagatca aaagcaaaac cactggcggc
    32821 ataacgacgc atgttctgaa taaatgcgcc cagttttgtt tcatcaccac aggtcttaaa
    32881 cagcgtgcgc cactcttctt ctgttcggtt gaggatcaat gccttgccga tgccggtgat
    32941 tgccagtggc atgcgatggc ctgggcgtga gcgcatttcc agcccgcgct gactgttgat
    33001 cttctccaga tacagcactt cgtcgccttc aacgatcccc agatgcacgg tgtccagggt
    33061 gtaacgggca aggcgttgca aaacgggctg ggcaatttcg tacagtgagg tgctttccag
    33121 tgctctggca ccgaattcga tcagtttggc acccagttgg tagccctgcg ttgggacatg
    33181 gcgcaggtag cgttgttcca ccagaacggt gactaaacga tgggttgtgc tccgggtcgt
    33241 gccggtgaac tcgccgatac tttttaggtc atggcagccg ttataaacag cattaagaac
    33301 cgccagtcca cgtaataacg tttgtgcgcc tgcggtggtt gaagaggttt ctgccatacg
    33361 tttctcctga tgatattgct gaagtgttcc acataatgac gtggggaaaa ggtgatatat
    33421 cccacaatat aaactccatc tcatattgtg ggataaaata atgaaaatta acgatcgttt
    33481 tgcgtagcgc ctcgcaggaa acttctttca aactggacgt ttgtacagca caattctatt
    33541 ttgtgcgggt aagttgttgg gtcaggaggc tttgtggatt tctcaatcat tgtttatgcc
    33601 attattgcgc tggttagtgt ggtcattggc tggctgtttg ccagttatca acatgcgcag
    33661 caaaaagccg agcaattagc tgaacgtgaa gagatggtcg cggagttaag cgcggcaaaa
    33721 caacaaatta ctcaaagcga gcactggcgc gctgagtgcg agttactcaa taacgaagtg
    33781 cgcagcctgc aaagtattaa cacctctctg gaggccgatc tgcgtgaagt aaccacgcgg
    33841 atggaagccg cacagcaaca tgctgacgat aaaattcgcc agatgatcaa cagcgagcag
    33901 cgcctcagtg agcagtttga aaacctcgcc aaccgtattt ttgagcacag caatcgccgg
    33961 gttgatgagc aaaaccgtca gagtctgaac agcctgttgt cgccgctacg tgaacaactg
    34021 gacggtttcc gccgtcaggt tcaggacagc ttcggtaaag aagcacagga acgtcatacc
    34081 ctgacccacg aaattcgcaa tctccagcag ctcaatgcgc aaatggctca ggaggcgatc
    34141 aacctgacgc gcgcgctgaa aggcgacaat aaaacccagg gcaactgggg cgaggtggta
    34201 ttgacgcggg tgctggaggc ttccggtctg cgtgaagggt atgaatatga aactcaggtc
    34261 agcatcgaaa atgacgcccg ctcgcggatg cagccggatg tcatcgtgcg gttgccgcag
    34321 ggaaaagatg tggtgatcga cgccaaaatg acgctggtcg cctatgaacg ctatttcaac
    34381 gccgaagatg actacacccg cgaaagcgcg ctacaggaac atatcgcgtc ggtgcgtaac
    34441 catatccgtt tgctgggacg caaagattat caacagctgc cggggctgcg aaccctggat
    34501 tacgtgctga tgtttattcc cgttgaaccc gcttttttac tggcgcttga ccgccagccg
    34561 gagctgatca ccgaagcgtt gaaaaacaat atcatgctgg ttagcccgac tacgctgctg
    34621 gtggcgctgc gcactatcgc caacctgtgg cgttatgagc atcaaagccg caacgcccag
    34681 caaatcgccg atcgtgccag caagctgtac gacaagatgc gtttgttcgt cgatgacatg
    34741 tcagcgattg gtcaaagtct cgacaaagcg caggataact accgccaggc aatgaaaaaa
    34801 ctctcttcag gtcgcggaaa tgtgctggcg caggcagaag cgtttcgcgg tttaggggta
    34861 gaaattaaac gcgagattaa tcctgatttg gctgaacagg cggtgagcca ggatgaagag
    34921 tatcgactgc ggtcggttcc gtagcagccg aatgatgaag cttatcaacg cgatgatgaa
    34981 tataatcagc agtcgcgcta gcccattggg ggtagttaag ccgggtagaa atctagggca
    35041 tcgacgccca atctgttaca cttctggaac aattttttga tgagcaggca ttgagatggt
    35101 ggataagtca caagaaacga cgcactttgg ttttcagacc gtcgcgaagg aacaaaaagc
    35161 ggatatggtc gctcacgttt tccattccgt ggcatcaaaa tacgatgtca tgaatgattt
    35221 gatgtcattc ggtattcatc gtttgtggaa gcgattcacg attgattgca gcggcgtacg
    35281 ccgtgggcag accgtgctgg atctggctgg tggcaccggc gacctgacag cgaaattctc
    35341 tcgcctggtc ggagaaactg gcaaagtggt ccttgctgat atcaatgaat ccatgctcaa
    35401 aatgggccgc gagaagctgc gtaatatcgg tgtgattggc aacgttgagt atgttcaggc
    35461 gaacgctgag gcgctgccgt tcccggataa cacctttgat tgcatcacca tttcgtttgg
    35521 tctgcgtaac gtcaccgaca aagataaagc actgcgttca atgtatcgcg tgctgaaacc
    35581 cggcggccgc ctgctggtgc ttgagttctc gaagccaatt atcgagccgc tgagcaaagc
    35641 ctatgatgca tactccttcc atgtgctgcc gcgtattggc tcactggtcg cgaacgacgc
    35701 cgacagctac cgttatctgg cagaatccat ccgtatgcat cccgatcagg ataccctgaa
    35761 aaccatgatg caggatgccg gattcgaaag tgtcgactac tacaatctga cggcaggggt
    35821 tgtggcgctg catcgtggtt ataagttctg acaggagacc ggaaatgcct tttaaacctt
    35881 tagtgacggc aggaattgaa agtctgctca acaccttcct gtatcgctca cccgcgctga
    35941 aaacggcccg ctcgcgtctg ttgggcaaag tgctgcgcgt ggaggtaaaa ggcttttcga
    36001 cgtcattgat tctggtgttc agcgaacgcc aggtcgatgt actgggcgaa tgggcaggcg
    36061 atgctgactg caccgttatc gcttacgcca gcgtgttgcc gaaacttcgc gatcgccagc
    36121 agcttgccgc actgattcgc agtggtgagc tggaagtgca gggcgatatt caggtggtgc
    36181 aaaacttcgt tgcgctggca gatctggctg agttcgaccc tgcggaactg ctggcccctt
    36241 ataccggtga tatcgccgct gaaggtatca gcaaagccat gcgcggaggc gcaaagtttc
    36301 tgcatcacgg cattaagcgc cagcaacgtt atgtggcgga agccattact gaagagtggc
    36361 gtatggcacc cggtccgctt gaagtggcct ggtttgcgga agagacggct gccgtcgagc
    36421 gtgctgttga tgccctgacc aaacggctgg aaaaactgga ggctaaatga cgccaggtga
    36481 agtacggcgc ctatatttca tcattcgcac ttttttaagc tacggacttg atgaactgat
    36541 ccccaaaatg cgtatcaccc tgccgctacg gctatggcga tactcattat tctggatgcc
    36601 aaatcggcat aaagacaaac ctttaggtga gcgactacga ctggccctgc aagaactggg
    36661 accggtatgg atcaagttcg ggcagatgtt atcaacccgc cgcgatcttt ttccgccgca
    36721 tattgccgat cagctggcgt tattgcagga caaagtcgct ccgtttgatg gcaagctggc
    36781 gaagcagcag attgaagctg caatgggcgg cttgccggta gaagcgtggt ttgacgattt
    36841 tgaaatcaag ccgctggctt ctgcttctat cgcccaggtt cataccgcgc gattgaaatc
    36901 gaatggtaaa gaggtggtga ttaaagttat ccgcccggat attttgccgg tcattaaagc
    36961 ggatctgaaa cttatttacc gtctggctcg ctgggtgccg cgtttgctgc cggatggtcg
    37021 ccgtctgcgc ccaaccgaag tggtgcgcga gtacgaaaag accttgattg atgaactgaa
    37081 tttgctgcgg gaatctgcca acgccattca gcttcggcgc aattttgaag acagcccgat
    37141 gctctacatc ccggaagttt accctgacta ttgtagtgaa gggatgatgg tgatggagcg
    37201 tatttacggc attccagtgt ctgatgttgc gacgctggag aaaaacggca ccaacatgaa
    37261 attgctggcg gaacgcggcg tgcaggtgtt cttcactcag gtctttcgcg acagcttttt
    37321 ccatgccgat atgcaccctg gcaacatctt cgtaagctat gaacacccgg aaaacccgaa
    37381 atatatcggc attgattgcg ggattgttgg ctcgctaaac aaagaagata aacgctatct
    37441 ggcggaaaac tttatcgcct tctttaatcg cgactatcgc aaagtggcag agctacacgt
    37501 cgattctggt tgggtgccac cagataccaa cgttgaagag ttcgaatttg ccattcgtac
    37561 ggtctgtgaa cctatctttg agaaaccgct ggccgaaatt tcgtttggac atgtactgtt
    37621 aaatctgttt aatacggcgc gtcgcttcaa tatggaagtg cagccgcaac tggtgttact
    37681 ccagaaaacc ctgctctacg tcgaaggggt aggacgccag ctttatccgc aactcgattt
    37741 atggaaaacg gcgaagcctt tcctggagtc gtggattaaa gatcaggtcg gtattcctgc
    37801 gctggtgaga gcatttaaag aaaaagcgcc gttctgggtc gaaaaaatgc cagaactgcc
    37861 tgaactggtt tacgacagtt tgcgccaggg caagtattta cagcatagtg ttgataagat
    37921 tgcccgcgag cttcagtcaa atcatgtacg tcagggacaa tcgcgttatt ttctcggaat
    37981 tggcgctacg ttagtattaa gtggcacatt cttgttggtc agccgacctg aatgggggct
    38041 gatgcccggc tggttaatgg caggtggtct gatcgcctgg tttgtcggtt ggcgcaaaac
    38101 acgctgattt tttcatcgct caaggcgggc cgtgtaacgt ataatgcggc cttgtttaat
    38161 catcatctac cacagaggaa catgtatggg tggtatcagt atttggcagt tattgattat
    38221 tgccgtcatc gttgtactgc tttttggcac caaaaagctc ggctccatcg gttccgatct
    38281 tggtgcgtcg atcaaaggct ttaaaaaagc aatgagcgat gatgaaccaa agcaggataa
    38341 aaccagtcag gatgctgatt ttactgcgaa aactatcgcc gataagcagg cggatacgaa
    38401 tcaggaacag gctaaaacag aagacgcgaa gcgccacgat aaagagcagg tgtaatccgt
    38461 gtttgatatc ggttttagcg aactgctatt ggtattcatc atcggcctcg tcgttctggg
    38521 gccgcaacga ctgcctgtgg cggtaaaaac ggtcgcgggc tggattcgcg cgttgcgttc
    38581 actggcgaca acggtgcaga acgaactgac ccaggagtta aaactccagg agtttcagga
    38641 cagtctgaaa aaggttgaaa aggcgagcct cactaacctg acgcccgaac tgaaagcgtc
    38701 gatggatgaa ctacgccagg ccgcggagtc gatgaagcgt tcctacgttg caaacgatcc
    38761 tgaaaaggcg agcgatgaag cgcacaccat ccacaacccg gtggtgaaag acaatgaagc
    38821 tgcgcatgaa ggcgtaacgc ctgctgctgc acaaacgcag gccagttcgc cggaacagaa
    38881 gccagaaacc acgccagagc cggtggtaaa acctgctgcg gacgctgaac cgaaaaccgc
    38941 tgcaccttcc ccttcgtcga gtgataaacc gtaaacatgt ctgtagaaga tactcaaccg
    39001 cttatcacgc atctgattga gctgcgtaag cgtctgctga actgcattat cgcggtgatc
    39061 gtgatattcc tgtgtctggt ctatttcgcc aatgacatct atcacctggt atccgcgcca
    39121 ttgatcaagc agttgccgca aggttcaacg atgatcgcca ccgacgtggc ctcgccgttc
    39181 tttacgccga tcaagctgac ctttatggtg tcgctgattc tgtcagcgcc ggtgattctc
    39241 tatcaggtgt gggcgtttat cgccccagcg ctgtataagc atgaacgtcg cctggtggtg
    39301 ccgctgctgg tttccagctc tctgttgttt tatatcggca tggcgttcgc ctactttgtg
    39361 gtctttccgc tggcatttgg cttccttgcc aataccgcgc cggaaggggt acaggtatcc
    39421 accgacatcg ccagctattt aagcttcgtt atggcgctgt ttatggcgtt tggtgtctcc
    39481 tttgaagtgc cggtggcaat cgtgctgctg tgctggatgg ggattacctc gccagaagac
    39541 ttacgcaaaa aacgcccgta tgtgctggtt ggtgcattcg ttgtcgggat gctgctgaca
    39601 ccgccggatg tcttctcgca aacgctgtta gcgatcccga tgtactgcct gtttgaaatc
    39661 ggtgtcttct tctcacgctt ttacgttggt aaagggcgaa atcgggaaga ggaaaacgac
    39721 gctgaagcag aaagcgaaaa aactgaagaa taaattcaac cgcccgtcag ggcggttgtc
    39781 atatggagta caggatgttt gatatcggcg ttaatttgac cagttcgcaa tttgcgaaag
    39841 accgtgatga tgttgtagca cgcgctcttg acgcgggagt taatgggcta ttcatcaccg
    39901 gcactaacct gcgtgaaagc cagcaggcgc aaaaactggc gcgtcagtat tcgtcctgtt
    39961 ggtcaacggc gggcgtacat cctcacgaca gcagccagtg gcaagctgcg actgaagaag
    40021 cgattattga gctggccgcg cagccagaag tggtggcgat tggtgaatgt ggtctcgact
    40081 ttaaccgcaa cttttcgacg ccggaagagc aggaacgcgc ttttgttgcc cagctacgca
    40141 ttgccgcaga attaaacatg ccggtattta tgcactgtcg cgatgcccac gagcggttta
    40201 tgacattgct ggagccgtgg ctggataaac tgcctggtgc ggttcttcat tgctttaccg
    40261 gcacacgcga agagatgcag gcgtgcgtgg cgcatggaat ttatatcggc attaccggtt
    40321 gggtttgcga tgaacgacgc ggactagagc tgcgggaact gttgccgttg attccggcgg
    40381 agaaattgct gatcgaaact gatgcgccgt atttgctccc gcgcgatctc acgccgaagc
    40441 catcatcccg gcgcaacgag ccagcccatc tgccccatat tttgcaacga attgcgcact
    40501 ggcgtggaga agatgccgca tggctggctg ccaccacgga tgccaatgtc aaaacactgt
    40561 ttgggattgc gttttagagt ttgcggaact cggtgttctt cacactgtgc ttaatctctt
    40621 tattgataag attaagtaac agcatggagc gagcctcgcc gtcgggttcg gtgaaaatgg
    40681 cctgaaaacc ttcgaacgcg ccttcggtaa taatcacctt atcacccgga taaggggttg
    40741 ccggatcgac aatgtctttc ggtttatata ccgatagctg atgaatcact gccgatggga
    40801 ctatcgctgg cgatgcgcca aagcgcacga agtggctgac accgcgggtc gcgttgatag
    40861 tcgtggtatg aatcacttct ggatcaaatt ccacaaacag gtagttgggg aacaatggct
    40921 cactgactgc agtacgtttg ccacgcacga ttttttccag ggtgatcatc ggtgccaggc
    40981 aattcacagc ctgtctttcg aggtgttcct gggcacgttg aagttgcccg cgcttgcagt
    41041 acagtaaata ccaggattgc ataatgactc ttatccgttt aatcggggcg caaggatagc
    41101 aaaagcttta cgctaagtta attatattcc ccggtttgcg ttatgccgtc agagttcacg
    41161 ctaatttaac aaatttacag catcgcaaag atgaacgccg tataatgggc gcagattaag
    41221 aggctacaat ggacgccatg aaatataacg atttacgcga cttcctgacg ctgcttgaac
    41281 agcagggtga gctaaaacgt atcacgctcc cggtggatcc gcatctggaa atcactgaaa
    41341 ttgctgaccg cactttgcgt gccggtgggc ctgcgctgtt gttcgaaaac cctaaaggct
    41401 actcaatgcc ggtgctgtgc aacctgttcg gtacgccaaa gcgcgtggcg atgggcatgg
    41461 ggcaggaaga tgtttcggcg ctgcgtgaag ttggtaaatt attggcgttt ctgaaagagc
    41521 cggagccgcc aaaaggtttc cgcgacctgt ttgataaact gccgcagttt aagcaagtat
    41581 tgaacatgcc gacaaagcgg ctgcgtggtg cgccttgcca acaaaaaatc gtctctggcg
    41641 atgacgtcga tctcaatcgt attcccatta tgacctgctg gccggaagat gctgcgccgc
    41701 tgattacctg ggggctcacc gtaacgcgcg gcccgcataa agagcggcag aatctgggca
    41761 tttatcgcca gcagctaatt ggtaaaaaca aactgattat gcgctggctg tcgcatcgcg
    41821 gcggcgcgtt ggattatcag gagtggtgtg cggcgcatcc gggcgaacgt ttcccggttt
    41881 ctgtggcgct gggtgccgat cctgccacga ttctcggtgc agtcaccccc gttccggata
    41941 cgctttcaga gtatgcgttt gccggattgc tgcgcggcac caagaccgaa gtggtgaagt
    42001 gtatctccaa tgaccttgaa gtgcccgcca gtgcggagat tgtgctggaa gggtatatcg
    42061 aacaaggcga aactgcgccg gaagggccgt atggcgacca caccggttac tataacgaag
    42121 tcgatagttt tccggtattt accgtgacgc atattaccca gcgtgaagat gcgatttatc
    42181 attccaccta taccgggcgt ccgccagatg aacctgcggt actgggtgta gcactgaacg
    42241 aagtgttcgt gccgattctg caaaaacagt tcccggaaat tgtcgatttt tatctgccgc
    42301 cggaaggctg ttcttatcgt ctggcggtag tgacgatcaa aaaacagtat gccggacacg
    42361 cgaagcgcgt catgatgggc gtctggtcgt tcttacgcca gtttatgtac actaaatttg
    42421 tgatcgtttg cgatgatgac gtcaacgccc gcgactggaa cgatgtgatt tgggcgatta
    42481 ccacccgtat ggacccggcg cgggataccg ttctggtaga aaatacgcct attgattatc
    42541 tggattttgc ctcccctgtc tccgggctgg gttcaaaaat ggggctggat gccacgaata
    42601 aatggccggg ggaaacccag cgtgaatggg gacgtcccat caaaaaagat ccagatgttg
    42661 tcgcgcatat tgacgccatt tgggatgaac tggctatttt taacaacggt aaaagcgcct
    42721 gatgcgcgtt tgttttgccc tatttatcga tccgacagag aaagcgcatg acaaccttaa
    42781 gctgtaaagt gacctcggta gaagctatca cggataccgt atatcgtgtc cgcatcgtgc
    42841 cagacgcggc cttttctttt cgtgctggtc agtatttgat ggtagtgatg gatgagcgcg
    42901 acaaacgtcc gttctcaatg gcttcgacgc cagatgaaaa agggtttatc gagctgcata
    42961 ttggcgcttc tgaaatcaac ctttacgcga aagcagtcat ggaccgcatc ctcaaagatc
    43021 atcaaatcgt ggtcgatatt ccccacggag aagcgtggct gcgcgatgat gaagagcgtc
    43081 cgatgatttt gattgcgggc ggcaccgggt tctcttatgc ccgctcgatt ttgctgacag
    43141 cgttggcgcg taacccaaac cgtgatatca ctatttactg gggcgggcgt gaagagcagc
    43201 atctgtatga tctctgcgag cttgaggcgc tttcgttgaa gcatcctggt ctacaagtgg
    43261 tgccggtggt tgaacaaccg gaagcgggct ggcgtgggcg tactggcacc gtgttaacgg
    43321 cggtattgca ggatcatggt acgctggcag agcatgatat ctatattgcc ggacgttttg
    43381 agatggcgaa aattgctcgt gacctgtttt gcagtgagcg taatgcgcgg gaagatcgtc
    43441 tgtttggcga tgcgtttgca tttatctgag atataaaaaa cccgcccctg acaggcggga
    43501 agaacggcaa ctaaaacgtt attcagtggc atttagatct atgtcgcatc cggcattcgg
    43561 cacacgctgc ctgatgcgac gctggcgcgt cttatcaggc ctacaattgg tgcaggatcg
    43621 gtagaccgga taaggcgttc acgccgcatc tggcaagtgg ttaaatccgc tcaaacaccg
    43681 tcgcaatacc ctgacccaga ccgatacaca tcgtcgcaag accaaactgg gcatccttgc
    43741 gctccatgag atttagcagc gtgctgctga tacgcgcacc ggaacagccc aacggatgac
    43801 ccagcgcgat cgcgccaccg ttgaggttga tcttctcgtc aatctgctca atcagtccca
    43861 gatctttaat acatggcagg atctgcgcgg caaaggcttc attcatttca aacacgccga
    43921 tatcactgac agaaagcccc gcttttttca gcgccaattt tgaggccgga accggaccgt
    43981 aacccataat cgacggatca caaccaacga ccgccatcga acgcacacgg gcgcgcggct
    44041 taagacctaa ttcatgggcg cggctttcac tcatcaccag catggcagct gcgccatcgg
    44101 aaagcgcaga agatgtaccc gccgttaccg taccgctgac gggatcaaac gccggacgca
    44161 gcgtggcgag ggcttccacg gtggtttccg ggcgaatcac ttcgtcgtaa ttaaactgct
    44221 tcaggacgcc gtcggcatcg tgaccaccgg tcgggataat ttcattttta aatgcacccg
    44281 actgcgtggc ggcccaggcg cgggcgtgtg accgtgcggc aaaggcatcc tgcatttcac
    44341 ggctgatacc gtgcatacgc gccagcattt ctgccgttaa gcccatcatg cccgccgctt
    44401 tggcgacatt acggctaagg ccggggtgaa aatcgacgcc gtgactcatc ggcacatggc
    44461 ccatatgctc cacgccgcca accagacatg cctgcgcatc gccggtcatg atcattcgtg
    44521 ctgcgtcatg cagtgcctgc atggatgaac cacacaagcg attgacagta accgccggga
    44581 cagagtgcgg cacttcagcc aacagtgccg cgttacgggc gatgttaaaa ccctgctcca
    44641 gcgtctgctg cacacaaccc cagtaaatat cgtcgagggc cgccgcttcc agcgccgggt
    44701 tacgcgccag caggctacgc attaaatgag cggagagatc ttccgcacgc acgtgacgaa
    44761 aagcaccgcc cttcgaacgg cccatcgggg tgcgaattgc atcgacaatg acaacctgtt
    44821 ccattgtgac tccttaagcc attttcaggt cgccaaccgg acgggctggc tcaaccgggg
    44881 gatagtacgg ttcgttatga cgcgctttat tacgcagacc ttccggcact tcatacagcg
    44941 ggccgagatg ctgatattgc tgtgccatat cgaggtattt tgcgctaccg agggtgtcca
    45001 gccagcggaa cgcgccgccg tgaaacggag ggaagcccag gccgtagacc agcgccatat
    45061 ccgcttctgc cggagtggcg ataatgcctt cttccagaca gcgcaccact tcgttgacca
    45121 tcgggatcat catgcgggcg ataatctctt cttcgctgaa atcgcgcttc ggctggctca
    45181 cttctgccag caggtcgtca acgacggcat cttcttcttt cttcggcttg cctttactgt
    45241 cttctttata acgccagaaa ccgaggccgt tcttctgacc aaagcggttg gcatcaaaca
    45301 gcgcgtcgat ggcatcgcgg taatctttct gcatccgctg cgggaagcct gctgccatca
    45361 ccgcctgagc gtgatgcgcg gtatcaatcc ccacaacgtc cagcagatat gccggcccca
    45421 tcggccagcc aaactgtttt tccatcactt tatcgatctt gcggaaatcc gcgccgtcgc
    45481 gcagcagctg gctgaaaccc gcgaagtagg ggaacagcac gcggttaacg aagaatccgg
    45541 ggcagtcgtt aaccacaatc ggcgttttgc ccatcttgct cgcccaggcg acaactttcg
    45601 cgatggtttc gtcggagctt ttctcgccgc gaataatttc caccaacggc attcggtgga
    45661 ccgggttaaa gaagtgcatc ccgcagaagt tttccggacg ctccagcgcg ttggccagtt
    45721 cgctgatagg aatggttgaa gtgttagacg caagcacggt atccgggcgt actttttgtt
    45781 cggtttctgc cagtacggct tttttcactt tcgggttttc gacaaccgct tctaccacaa
    45841 catctacgcg gtcaaaacca gagtaatcga gcgttgggtg gatcgtggag atcacgccag
    45901 ccagtttcag accatcgatc ttgccgcgct caagctgctt gttcagcagt ttcgcggctt
    45961 cggtcatgcc gagggttaac gacttgtcgt tgatatcttt catgacaacc ggcacgcctt
    46021 tccacgcaga ctggtaagcg atgccgccgc ccataatgcc tgcgcccagc acagcggccc
    46081 gtttcggcgt ttcaacgtct ttggtgagtt tcttcgcttt gccttttaca tattgatcgt
    46141 taaggaatat gccgaccaat gcgcgagctt cgttggtatg cgccagcggg acaaaacttt
    46201 tgttttccag gtttagggct tcttcacgac caaaacgggc cgcagcttca atggttttta
    46261 ctgcggtgat gggggccgga taatgtttcc ccgccgtttg tgcgaccatc cctttagcga
    46321 tggtgaagct catgatggct tcaatcttgc tcagttttag cggttccagc ttcggctgac
    46381 gtttcgcttt ccagtcgagg tcgccgttaa tggcctggcg taaaatcgcc atcgcccctt
    46441 caatcagttt ttctgctttg actacaccat ccaccagacc gattttcagc gcctgatccg
    46501 cgccgacatc tttaccggcg gcaatgattt ccagcgcact gtcagcgccc agcatacgtg
    46561 gcatacgtac agaaccgcca aagcccggca tgatgcccag tttggtttcc ggcagaccga
    46621 tgcgcagatc cggcgtcgcc agacgataat cggtcgccag cacgcattcg cagccaccgc
    46681 ccagcgcata gccgttgacg gcagcaatgg tcggcaccgg gagatcttcc aggcgattaa
    46741 acacgctatt ggcaaagtgc agccactgac ttaactgttc ttcaggaacg aggaacaggg
    46801 acaaaaattc ggtgatatca gcaccgacga taaaggctgc tttgttcgaa cgcagcagca
    46861 gcccttttag atctgattgc tgttccagca cgccgatggc ctcgccgagg ctggcgacgg
    46921 tcgcagtgtc gagtttatta actgaacctg gggcatcaaa taccagttcg gcaatgccat
    46981 cttccagcca gtcaaggtac agggtgtcgc ctttgtaaag catgtcagtc tcctgaatcc
    47041 gcaaggtgat ctggtcgtac cagatgcatc gaagtgtgta ttttgtgtta aaaatatgca
    47101 aataaaagat taaagaaatg ccgatctgat cacgctcggc agaaatcacg ctctggatga
    47161 acgatgtgct aagatgcgga gacttaaggt caaaaaaaca gaagggtaaa aaatggaatc
    47221 actggcctca ctctataaaa atcatatagc taccttacaa gaacggactc gcgatgcgct
    47281 ggcgcgcttc aagctggatg cgttacttat tcactccggc gaactgttca acgtttttct
    47341 cgacgatcat ccctatccgt ttaaagtgaa tccgcaattc aaagcgtggg tgccggtaac
    47401 tcaggtgcca aactgctggc tgttggtgga tggcgtgaac aagccgaaac tgtggtttta
    47461 tctgccggtt gattactggc acaacgtcga accgctgccg acctccttct ggactgaaga
    47521 tgtagaagtg atcgcgctgc cgaaagccga tggcattggt agcctgctgc ctgctgcgcg
    47581 cgggaatatc ggttatatcg gtccggtgcc ggagcgtgcg ctgcaactgg gtattgaggc
    47641 cagcaatatc aacccgaaag gggtgatcga ctacctgcat tactaccgct ccttcaaaac
    47701 cgagtacgaa ctggcctgta tgcgtgaagc gcagaaaatg gcggtcaacg gtcatcgcgc
    47761 ggcagaagaa gcgttccgtt ctggcatgag cgagttcgat atcaatattg cctatctgac
    47821 cgcgaccggt catcgtgata ccgacgtacc ttacagcaac attgtggcgc ttaacgaaca
    47881 cgctgcggtg ttgcattaca ccaaactgga ccaccaggca ccggaagaga tgcgcagctt
    47941 cctgctggat gccggggccg aatataacgg ctatgcggct gacctgaccc gtacctggtc
    48001 ggcaaaaagc gacaacgact acgcacagct ggtgaaagac gtaaatgatg agcaactggc
    48061 gctgatcgcc accatgaaag caggcgtcag ctatgtggat taccatatcc agttccatca
    48121 gcgcatcgcc aaactgctgc gtaaacatca aatcatcacc gatatgagtg aagaagcgat
    48181 ggtcgaaaac gatctcaccg gaccgtttat gccgcacggt atcggccatc cgctgggcct
    48241 gcaggtgcat gacgtagccg gtttcatgca ggatgatagc ggtacgcacc tcgcggcacc
    48301 ggcaaaatat ccgtacctgc gctgcacccg tattctccag ccgggcatgg tgttaaccat
    48361 cgagccgggt atctacttca tcgaatcgct actggcaccg tggcgtgaag ggcagttcag
    48421 taaacacttt aactggcaga aaattgaagc actgaaaccg ttcggcggca ttcgtatcga
    48481 agacaacgtg gtgatccacg aaaacaacgt ggagaacatg acccgggatc tgaaactggc
    48541 gtgatggaaa gctggttaat tcctgcggca ccggtcacgg tcgttgaaga gattaaaaag
    48601 agccgcttca ttacgctgtt ggcgcatacc gatggcgttg aggcggcgaa agcgtttgtt
    48661 gaatcggtgc gagcagaaca ccccgatgcc cgccaccatt gcgtggcgtg ggtggcgggt
    48721 gcgcctgacg attcacaaca gctgggtttc tctgacgacg gggagccgac gggaacggca
    48781 ggtaaaccga tgctcgccca actaatgggc agcggcgtcg gggaaattac cgctgtggtg
    48841 gtgcgctact acggcggcat attgcttggc accggtgggt tagtgaaagc gtatggcggc
    48901 ggcgtgaatc aggcgctgcg ccagctaacg acccaacgca agacaccatt aaccgaatat
    48961 actttgcaat gtgaatatag tcagttaacc ggcattgaag cgttgctggg gcagtgtgac
    49021 ggcaaaatta tcaacagtga ttatcaggca ttcgttctgc tgcgggtggc gcttccggcg
    49081 gcgaaagtgg ctgaattttc cgcaaagctg gcggatttta gccgtggttc attgcaattg
    49141 ttagcgattg aagaataatc cccacttcgt tttgcagaac taaggaagcg gcagagatgc
    49201 attttcgcgc cattacccga atcgttggac tactggtcat cttattttca gggaccatga
    49261 ttatccctgg gctggtagca ctcatctacc gggatggagc gggccgcgct tttacccaga
    49321 ccttttttgt cgccctcgcc attggctcta tgctttggtg gccgaaccgc aaagagaagg
    49381 gtgaactgaa atcccgtgag gggtttctga tagtggtgct gttctggacc gtgctgggta
    49441 gcgtcggtgc gctccctttt atcttctcgg aaagcccgaa cctcacgatt accgatgcgt
    49501 tttttgaatc tttctctggc ctgaccacca cgggggccac tacgctagtg gggctggatt
    49561 cgctccctca cgccatcctc ttttatcgcc agatgctgca atggtttggc gggatgggga
    49621 tcatcgtatt agcggttgcg atactgccta tcctcggcgt gggtgggatg cagctctatc
    49681 gcgcagaaat gcccggcccg ctgaaagata acaaaatgcg cccgcgaatt gcggaaacgg
    49741 cgaaaaccct gtggttgatt tatgtcttgc tgaccgtcgc ctgtgcgctg gcgttgtggt
    49801 ttgccggaat ggatgccttt gatgccatcg gccatagctt tgcgactatc gctattggcg
    49861 gcttctcgac acatgatgcc agtatcggtt atttcgacag cccgactatt aacactatca
    49921 ttgctatctt cctgctgatc tccggctgta actacggtct gcacttttca ctgttaagtg
    49981 ggcgtagtct gaaggtttat tggcgcgatc cggaatttcg catgtttatc ggcgtacagt
    50041 ttacgctggt ggttatttgt acactcgtgc tgtggtttca taatgtctac agttcggcgc
    50101 tgatgacaat taaccaggcg tttttccagg tggtgtcgat ggcgacaacc gccgggttta
    50161 cgactgacag cattgcccgc tggccgctct ttttgccggt actgctctta tgttcagctt
    50221 ttatcggcgg ttgtgccggg tcaacgggcg gtggcctgaa agtgatccgc atcctgctgc
    50281 tgtttaagca ggggaaccgt gagctgaaac gactggtgca tccgaacgcc gtctacagca
    50341 ttaagctggg gaatcgcgca ctgccggaac gtatcctcga agccgtttgg ggatttttct
    50401 ccgcctatgc attggtgttt attgtcagta tgctggcgat tatcgccacg ggcgtggatg
    50461 acttttctgc ctttgcctcg gttgttgcaa cattgaataa cctggggcca gggcttggcg
    50521 tggttgctga taactttacc agtatgaacc cggtggctaa atggatcctg attgccaaca
    50581 tgctgtttgg tcgtctcgag gtctttacat tgctggtgct ctttaccccg actttctggc
    50641 gtgaatgatg gagtaatacg tgaaaacatt aattcttttc tcaacaaggg acggacaaac
    50701 gcgcgagatt gcctcctacc tggcttcgga actgaaagaa ctggggatcc aggcggatgt
    50761 cgccaatgtg caccgcattg aagaaccaca gtgggaaaac tacgaccgtg tggtcattgg
    50821 tgcttctatt cgctatggtc actaccactc tgcgttccag gaatttgtca aaaagcatgc
    50881 gacgcggctg aattcgatgc cgagcgcctt ttactccgta aacctggtgg cgcgtaaacc
    50941 ggagaagcgt actccacaga ccaacagcta cgcgcggaag tttctgatga actcgcaatg
    51001 gcgtcccgat cgctgcgcgg tcattgccgg ggcgctgcgt tatccacgtt accgctggta
    51061 cgaccgcttt atgatcaaac tgattatgaa gatgtcaggc ggtgaaacgg atacgcgcaa
    51121 agaagttgtc tataccgatt gggagcaggt ggcgaatttc gcccgagaaa tcgcccattt
    51181 aaccgacaaa ccgacgctga aataagcata aagaataaaa aatgcgcggt cagaaaatta
    51241 ttttaaattt cctcttgtca ggccggaata actccctata atgcgccacc actgacacgg
    51301 aacaacggca aacacgccgc cgggtcagcg gggttctcct gagaatctca acagagaaaa
    51361 gcaaaaaaat gcttgactct gtagcgggaa
//
LOCUS       DAPHCV010000018        50735 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.018, whole genome shotgun sequence.
ACCESSION   DAPHCV010000018 DAPHCV010000000
VERSION     DAPHCV010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.018"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            204..1151
                     /gene="cyoA"
                     /locus_tag="RH033_001061"
     CDS             204..1151
                     /gene="cyoA"
                     /locus_tag="RH033_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HDV2990285.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            1173..3164
                     /gene="cyoB"
                     /locus_tag="RH033_001062"
     CDS             1173..3164
                     /gene="cyoB"
                     /locus_tag="RH033_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HDV2990286.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMYKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            3154..3768
                     /locus_tag="RH033_001063"
     CDS             3154..3768
                     /locus_tag="RH033_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HDV2990287.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3768..4097
                     /locus_tag="RH033_001064"
     CDS             3768..4097
                     /locus_tag="RH033_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HDV2990288.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            4109..4999
                     /gene="cyoE"
                     /locus_tag="RH033_001065"
     CDS             4109..4999
                     /gene="cyoE"
                     /locus_tag="RH033_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HDV2990289.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            5148..6512
                     /locus_tag="RH033_001066"
     CDS             5148..6512
                     /locus_tag="RH033_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2990290.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETAGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(6640..7131)
                     /gene="yajQ"
                     /locus_tag="RH033_001067"
     CDS             complement(6640..7131)
                     /gene="yajQ"
                     /locus_tag="RH033_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HDV2990291.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            7299..8210
                     /gene="panE"
                     /locus_tag="RH033_001068"
     CDS             7299..8210
                     /gene="panE"
                     /locus_tag="RH033_001068"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDV2990292.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTATWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            8173..8763
                     /gene="yajL"
                     /locus_tag="RH033_001069"
     CDS             8173..8763
                     /gene="yajL"
                     /locus_tag="RH033_001069"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HDV2990293.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLIETVKQFHRSGRIV
                     AAICATPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(8817..10265)
                     /gene="thiI"
                     /locus_tag="RH033_001070"
     CDS             complement(8817..10265)
                     /gene="thiI"
                     /locus_tag="RH033_001070"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDV2990294.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQAE
                     QEVVEVETVNDFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            10471..10713
                     /gene="xseB"
                     /locus_tag="RH033_001071"
     CDS             10471..10713
                     /gene="xseB"
                     /locus_tag="RH033_001071"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDV2990295.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            10713..11612
                     /gene="ispA"
                     /locus_tag="RH033_001072"
     CDS             10713..11612
                     /gene="ispA"
                     /locus_tag="RH033_001072"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HDV2990296.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LTEQSLDTSALEALADYIIQRNK"
     gene            11637..13499
                     /gene="dxs"
                     /locus_tag="RH033_001073"
     CDS             11637..13499
                     /gene="dxs"
                     /locus_tag="RH033_001073"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDV2990297.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALVLEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDATGMEAKIKAWLA"
     gene            13757..14731
                     /gene="yajO"
                     /locus_tag="RH033_001074"
     CDS             13757..14731
                     /gene="yajO"
                     /locus_tag="RH033_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HDV2990298.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(14785..15303)
                     /gene="pgpA"
                     /locus_tag="RH033_001075"
     CDS             complement(14785..15303)
                     /gene="pgpA"
                     /locus_tag="RH033_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDV2990299.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(15281..16258)
                     /gene="thiL"
                     /locus_tag="RH033_001076"
     CDS             complement(15281..16258)
                     /gene="thiL"
                     /locus_tag="RH033_001076"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HDV2990300.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(16336..16755)
                     /gene="nusB"
                     /locus_tag="RH033_001077"
     CDS             complement(16336..16755)
                     /gene="nusB"
                     /locus_tag="RH033_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDV2990301.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(16775..17245)
                     /gene="ribE"
                     /locus_tag="RH033_001078"
     CDS             complement(16775..17245)
                     /gene="ribE"
                     /locus_tag="RH033_001078"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDV2990302.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(17334..18437)
                     /gene="ribD"
                     /locus_tag="RH033_001079"
     CDS             complement(17334..18437)
                     /gene="ribD"
                     /locus_tag="RH033_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDV2990303.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHH
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARLDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPIRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGNDARGLC
                     TMPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(18441..18890)
                     /gene="nrdR"
                     /locus_tag="RH033_001080"
     CDS             complement(18441..18890)
                     /gene="nrdR"
                     /locus_tag="RH033_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HDV2990304.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            19041..19580
                     /locus_tag="RH033_001081"
     CDS             19041..19580
                     /locus_tag="RH033_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HDV2990305.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     MSVDQLGFVRIHDIQPVMQ"
     gene            19879..20763
                     /gene="tsx"
                     /locus_tag="RH033_001082"
     CDS             19879..20763
                     /gene="tsx"
                     /locus_tag="RH033_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HDV2990306.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(20940..21287)
                     /locus_tag="RH033_001083"
     CDS             complement(20940..21287)
                     /locus_tag="RH033_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HDV2990307.1"
                     /translation="MAIIPKNYARLESDYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(21416..22387)
                     /gene="secF"
                     /locus_tag="RH033_001084"
     CDS             complement(21416..22387)
                     /gene="secF"
                     /locus_tag="RH033_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HDV2990308.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(22398..24245)
                     /gene="secD"
                     /locus_tag="RH033_001085"
     CDS             complement(22398..24245)
                     /gene="secD"
                     /locus_tag="RH033_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HDV2990309.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPSEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(24273..24605)
                     /gene="yajC"
                     /locus_tag="RH033_001086"
     CDS             complement(24273..24605)
                     /gene="yajC"
                     /locus_tag="RH033_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDV2990310.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(24628..25755)
                     /gene="tgt"
                     /locus_tag="RH033_001087"
     CDS             complement(24628..25755)
                     /gene="tgt"
                     /locus_tag="RH033_001087"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDV2990311.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(25811..26881)
                     /gene="queA"
                     /locus_tag="RH033_001088"
     CDS             complement(25811..26881)
                     /gene="queA"
                     /locus_tag="RH033_001088"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDV2990312.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKSAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            27177..27758
                     /gene="acpH"
                     /locus_tag="RH033_001089"
     CDS             27177..27758
                     /gene="acpH"
                     /locus_tag="RH033_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HDV2990313.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRNETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFT
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQSVLNGMASRRPRLDALR
                     NSWYDLDAHYDALETRFWQFYPRMMEQASRKAL"
     gene            complement(27763..29580)
                     /gene="malZ"
                     /locus_tag="RH033_001090"
     CDS             complement(27763..29580)
                     /gene="malZ"
                     /locus_tag="RH033_001090"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HDV2990314.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLIRLWLTGEDPPQRIMLRTEHDN
                     EETSVPMHKQRSQPQPGVTAWRAAIDLSSGLPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIAPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLYQQRVLVAINRGEACEV
                     VLPASPLLNVAQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            complement(29736..31109)
                     /gene="proY"
                     /locus_tag="RH033_001091"
     CDS             complement(29736..31109)
                     /gene="proY"
                     /locus_tag="RH033_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HDV2990315.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGVAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKVFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(31185..32504)
                     /gene="brnQ"
                     /locus_tag="RH033_001092"
     CDS             complement(31185..32504)
                     /gene="brnQ"
                     /locus_tag="RH033_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HDV2990316.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(32911..34206)
                     /gene="phoR"
                     /locus_tag="RH033_001093"
     CDS             complement(32911..34206)
                     /gene="phoR"
                     /locus_tag="RH033_001093"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HDV2990317.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLHIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(34264..34953)
                     /gene="phoB"
                     /locus_tag="RH033_001094"
     CDS             complement(34264..34953)
                     /gene="phoB"
                     /locus_tag="RH033_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HDV2990318.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            35143..36345
                     /gene="sbcD"
                     /locus_tag="RH033_001095"
     CDS             35143..36345
                     /gene="sbcD"
                     /locus_tag="RH033_001095"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HDV2990319.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQAHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITEQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELEESQQQRLQHLFATTLH
                     SLAGEHEA"
     gene            36342..39485
                     /gene="sbcC"
                     /locus_tag="RH033_001096"
     CDS             36342..39485
                     /gene="sbcC"
                     /locus_tag="RH033_001096"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HDV2990320.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISSMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNTLAAITLTLTADEVASALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQARLLTLEKEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRMQQLTPILETLPQSDELPHSEETVALENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTQQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESAFAVK"
     gene            39611..40795
                     /gene="araJ"
                     /locus_tag="RH033_001097"
     CDS             39611..40795
                     /gene="araJ"
                     /locus_tag="RH033_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HDV2990321.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNVLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFFGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(40934..41842)
                     /gene="mak"
                     /locus_tag="RH033_001098"
     CDS             complement(40934..41842)
                     /gene="mak"
                     /locus_tag="RH033_001098"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HDV2990322.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            41967..42878
                     /gene="rdgC"
                     /locus_tag="RH033_001099"
     CDS             41967..42878
                     /gene="rdgC"
                     /locus_tag="RH033_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HDV2990323.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(42956..43039)
                     /locus_tag="RH033_001100"
     CDS             complement(42956..43039)
                     /locus_tag="RH033_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2990324.1"
                     /translation="MTQRPWSKLQRKTHNIAALKIIARRSE"
     gene            complement(43036..43374)
                     /locus_tag="RH033_001101"
                     /pseudo
     CDS             complement(43036..43374)
                     /locus_tag="RH033_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(43522..43806)
                     /gene="ppnP"
                     /locus_tag="RH033_001102"
     CDS             complement(43522..43806)
                     /gene="ppnP"
                     /locus_tag="RH033_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HDV2990325.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(43878..44555)
                     /locus_tag="RH033_001103"
     CDS             complement(43878..44555)
                     /locus_tag="RH033_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HDV2990326.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVTVLLSNVLIAR
                     LAAELLV"
     gene            complement(44813..45004)
                     /gene="yaiA"
                     /locus_tag="RH033_001104"
     CDS             complement(44813..45004)
                     /gene="yaiA"
                     /locus_tag="RH033_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HDV2990327.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(45054..45578)
                     /gene="aroL"
                     /locus_tag="RH033_001105"
     CDS             complement(45054..45578)
                     /gene="aroL"
                     /locus_tag="RH033_001105"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HDV2990328.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(45761..46219)
                     /locus_tag="RH033_001106"
     CDS             complement(45761..46219)
                     /locus_tag="RH033_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDV2990329.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELDKWWLEVQRSRG"
     gene            46339..47148
                     /gene="proC"
                     /locus_tag="RH033_001107"
     CDS             46339..47148
                     /gene="proC"
                     /locus_tag="RH033_001107"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDV2990330.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(47165..48265)
                     /gene="adrA"
                     /locus_tag="RH033_001108"
                     /gene_synonym="dgcC"
     CDS             complement(47165..48265)
                     /gene="adrA"
                     /locus_tag="RH033_001108"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HDV2990331.1"
                     /translation="MNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLPRAVGL
                     AGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNLKTDAV
                     LAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLELTGITV
                     SFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGVYNRRH
                     WETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQITLRG
                     SDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVGVAPLN
                     PQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(48381..48701)
                     /gene="psiF"
                     /locus_tag="RH033_001109"
     CDS             complement(48381..48701)
                     /gene="psiF"
                     /locus_tag="RH033_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HDV2990332.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(48820..50235)
                     /gene="phoA"
                     /locus_tag="RH033_001110"
     CDS             complement(48820..50235)
                     /gene="phoA"
                     /locus_tag="RH033_001110"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HDV2990333.1"
                     /translation="MKQSTIALALLPLLFSPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(50335..50595)
                     /gene="iraP"
                     /locus_tag="RH033_001111"
     CDS             complement(50335..50595)
                     /gene="iraP"
                     /locus_tag="RH033_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HDV2990334.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLRHPRQ"
BASE COUNT        12345 a        13519 c        12870 g        12001 t
ORIGIN      
        1 agactccttc tgcactcccc ttttgttata acgccctttt gcaacagctt cttaaaatca
       61 acctgatatg ttttgcaaca tatgtgacct ggcagccaaa tctaagtaac aggaatttaa
      121 tcatgtttac agtaatgtaa ccttcccgta aaatgcccac acactttaaa cgccaccaga
      181 tcccgtggaa ttgaggtcgt taaatgagac tcaggaaata caataaaagt ttgggatggt
      241 tgtcattatt tgcaggcact gtattgctca gtggctgtaa ttctgcgctg ttagatccca
      301 aaggacagat tggtctggag caacgttcac tgatactgac ggcatttggc ctgatgttga
      361 ttgtcgttat tcccgcaatc ttgatggctg ttggtttcgc ctggaagtat cgtgcgagca
      421 ataaagatgc taagtacagc ccgaactggt cacactccaa taaagtggaa gctgtggtct
      481 ggacggtacc tatcttaatc atcatcttcc ttgcggtact gacctggaaa accactcacg
      541 ctcttgagcc tagcaagccg ctggcacacg acgagaagcc cattaccatc gaagtggttt
      601 ccatggactg gaaatggttc ttcatctacc cggaacaggg cattgctacc gtgaatgaaa
      661 tcgctttccc ggcgaacact ccggtgtact tcaaagtgac ctccaactcc gtgatgaact
      721 ccttcttcat tccgcgtctg ggtagccaga tttatgccat ggccggtatg cagactcgcc
      781 tgcatctgat cgccaacgaa cccggtactt atgacggtat ctccgccagc tacagcggcc
      841 cgggcttctc aggcatgaag ttcaaagcta ttgctacacc ggatcgcgcc gcattcgacc
      901 agtgggtcgc aaaagcgaaa cagtcgccga acaccatgtc tgacatggca gcgttcgaaa
      961 aactggccgc gcctagcgaa tacaaccagg tggaatattt ctccaacgtg aaaccagact
     1021 tgtttgctga tgtgattaac aagtttatgg ctcacggtaa gagcatggac atgacccagc
     1081 cagaaggtga gcacagcgca cacgaaggta tggaaggcat ggacatgagc cacgcggaat
     1141 ccgcccatta aaggggttga ggaagaataa agatgttcgg aaaattatca cttgatgcag
     1201 tcccgttcca tgaacctatc gtcatggtta cgatcgctgg cattattttg ggaggtctgg
     1261 cgctcgttgg cctgatcact tacttcggta agtggaccta cctgtggaaa gagtggctga
     1321 cctccgtcga ccataaacgc ctcggtatca tgtatatcat cgtggcgatt gtgatgttgc
     1381 tgcgtggttt tgctgacgcc attatgatgc gtagccagca ggctcttgcc tcggcgggcg
     1441 aagcgggctt cctgccacct caccactacg atcagatctt caccgcgcac ggcgtgatta
     1501 tgatcttctt cgtggcgatg cctttcgtta tcggtctgat gaacctggtg gttccgctgc
     1561 agatcggcgc gcgtgacgtt gcgttcccgt tcctcaacaa cctaagcttc tggtttaccg
     1621 ttgttggtgt gattctggtt aacgtttctc tcggcgtggg cgaatttgcg cagaccggct
     1681 ggctggccta tccaccgcta tcgggaatag agtacagtcc gggagtcggt gtcgattact
     1741 ggatatggag tctccagcta tccggtatag gtacaacgct taccggtatc aacttcttcg
     1801 ttaccattct gaagatgcgc gcaccgggca tgaccatgta caagatgcca gtatttacct
     1861 gggcatcact gtgcgcgaac gtactgatta ttgcttcctt cccaattctg acggttaccg
     1921 ttgcgttgtt gaccctggat cgctatctgg gcacccattt ctttaccaac gatatgggtg
     1981 gcaacatgat gatgtacatc aacctgattt gggcctgggg ccacccggaa gtttacatcc
     2041 tgatcctgcc tgttttcggt gtgttctccg aaattgcggc aaccttctcg cgtaaacgtc
     2101 tgtttggtta tacctcgctg gtatgggcaa ccgtctgtat caccgtgctg tcgttcatcg
     2161 tctggctgca ccacttcttt acgatgggtg cgggcgcgaa cgtaaacgcc ttctttggta
     2221 tcaccaccat gattatcgcc atcccgaccg gggtgaagat cttcaactgg ctgttcacca
     2281 tgtatcaggg ccgcatcgtg ttccattctg cgatgctgtg gaccatcggt tttatcgtca
     2341 ccttctcggt aggcggtatg actggcgtgc tgctggcagt accgggcgcg gacttcgttc
     2401 tgcataacag cctgttcctg attgcgcact tccataacgt gatcatcggc ggcgtggtct
     2461 tcggctgctt cgcagggatg acctactggt ggcctaaagc gttcggtttc aaactgaacg
     2521 aaacctgggg taaacgcgcg ttctggttct ggatcatcgg cttcttcgtt gcctttatgc
     2581 cgctgtatgc gctgggcttc atgggcatga cccgtcgttt gagccagcag attgacccgc
     2641 agttccacac catgctgatg attgcagcca gcggtgcagt actgattgcg ctgggtattc
     2701 tctgcctcgt tattcagatg tacgtttcta ttcgtgaccg cgaccagaac cgtgacctga
     2761 ctggcgaccc gtggggtggc cgtacgctgg agtgggcaac ctcttccccg cctccgttct
     2821 ataactttgc cgttgtgccg cacgttcacg agcgtgatgc attctgggaa atgaaagaga
     2881 aaggcgaagc gtacaaaaag cctgaccact atgaagaaat tcatatgccg aaaaacagcg
     2941 gtgcaggtat cgtcattgca gctttctcca ccatcttcgg tttcgccatg atctggcata
     3001 tctggtggct ggcgattgtt ggcttcgcag gcatgatcat cacctggatc gtgaaaagct
     3061 tcgacgagga cgtggattac tacgtgccgg tggcagaaat cgaaaaactg gaaaaccagc
     3121 atttcgatga gattactaag gcagggctga aaaatggcaa ctgatacttt gacgcacgcg
     3181 actgcccacg cgcacgaaca cgggcaccac gatgcaggcg gaaccaaaat cttcggcttt
     3241 tggatctacc tgatgagcga ctgcattctg ttctctatct tgtttgctac ctatgccgtt
     3301 ctggttaacg gcaccgcagg cggcccgaca ggtaaggaca ttttcgaact gccgttcgtt
     3361 ctggttgaaa ctttcttgct gttgttcagc tccatcacat acggcatggc ggctatcgcc
     3421 atgtacaaaa acaacaaaag ccaggttatc tcctggctgg cgttgacctg gttgtttggt
     3481 gctggattta tcgggatgga aatctatgaa ttccatcacc tgattgttaa cggcatgggt
     3541 ccggatcgca gcggcttcct gtcagcgttc tttgctctgg tcggcacgca cggtctgcac
     3601 gtcacttccg gtcttatctg gatggcggtg ctgatggtgc aaatcgcccg tcgcggcctg
     3661 accagcacta accgtacccg catcatgtgc ctgagcctgt tctggcactt cctggatgtg
     3721 gtttggatct gtgtgttcac tgttgtttat ctgatggggg cgatgtaatg agtcattcta
     3781 ccgatcacag cggcgcgtcc catggcagcg taaaaaccta catgacaggc tttatcctgt
     3841 cgatcattct gacggtgatt ccgttctgga tggtgatgac aggagctgcc tctccggccg
     3901 taattctggg aacaatcctg gcaatggcag tggtacaggt tctggtgcat ctggtgtgct
     3961 tcctgcacat gaataccaaa tcagatgaag gctggaacat gacggcgttt gtcttcaccg
     4021 tgctaatcat cgctatcctg gttgtaggct ccatctggat tatgtggaac ctcaactaca
     4081 acatgatgat gcactaagag cggcggttat gatgtttaag caatacctgc aagtaacgaa
     4141 accaggcatc atctttggca acctgatctc ggtgattggg ggattcctgc tggcctcaaa
     4201 gggcagcatt gattatcccc tgtttatcta cacgctggtt ggggtgtcac tggttgtggc
     4261 gtcgggttgt gtgtttaaca actacatcga cagggatatc gacagaaaga tggaaaggac
     4321 gaagaatcgg gtgctggtta aaggcctgat ctctcctgct gtctcgctgg tgtacgccac
     4381 cttgctgggt attgctggct ttatgctgct gtggtttggc gcgaatccgc tggcctgctg
     4441 gctgggggtg atgggctttg tggtttacgt cggcgtttat agcctgtaca tgaaacgcca
     4501 ctcagtctac ggcacattga ttggttcgct ctccggcgct gcgccgccgg tgatcggcta
     4561 ctgtgcagta accggcgagt tcgatagtgg cgcagcgatc ctgcttgcta tcttcagtct
     4621 gtggcagatg cctcactcct atgccatcgc cattttccgc tttaaggatt accaggcggc
     4681 aaacattccg gtattgccag tggtaaaagg catttcggtg gcgaagaatc acatcacgct
     4741 gtatatcatc gcctttgccg ttgccacgct gatgctctct cttggcggtt acgctgggta
     4801 taaatatctg gtagtcgccg cggcggtgag cgtctggtgg ttaggcatgg ctctgcgcgg
     4861 ttataaagtt gctgatgaca gaatctgggc gcgcaagctg ttcggcttct ctatcatcgc
     4921 catcactgcc ctctcggtga tgatgtccgt tgattttatg gtgccggact cgcatacgct
     4981 gctggctgct gtgtggtaac acaacctctc agttaaaaag gtgctacggc accttttttc
     5041 ttagcattag aatcatatcc ctctcgaaat atttactaaa aaatccgcat gtttacccca
     5101 ttcgtttgcc gctttacact agtcgcgaat ttaaaacaga ggtggtaatg aacgattata
     5161 aaatgacgcc aggtgagcga cgcgcgacct ggggtttagg gaccgtattc tcgttgcgca
     5221 tgctgggcat gttcatggtt ctgccggttc tgaccacgta cggcatggct ctgcaaggtg
     5281 ccagcgaagc attaatcggt attgccattg gtatttatgg tctgactcag gccgtttttc
     5341 agattccgtt tggcctgctt tccgaccgca ttggtcgcaa accattaatt gtcggtgggc
     5401 tggcagtgtt tgccgccggt agcgttatcg ctgcgctctc tgactccatc tggggaatta
     5461 ttctgggccg ggcgctacaa ggctccggtg cgattgccgc tgccgttatg gcgctgcttt
     5521 ccgatctcac gcgcgaacaa aaccgcacca aagcaatggc gtttatcggc gtgagctttg
     5581 gcattacctt tgccattgcg atggtgcttg gcccgatcat cactcacaaa cttgggctgc
     5641 acgcgctgtt ctggatgatc gctattctgg caacgaccgg cattgcgttg accatttggg
     5701 ttgtgcccaa cagtagcact cacgtactta atcgtgagtc cggaatggtg aaaggcagtt
     5761 tcagtaaagt gctggcggaa ccgcggctgc tgaaactcaa ctttggcatt atgtgtctgc
     5821 atattttgct gatgtcgacg tttgttgccc tgcccggaca actggctgat gcggggttcc
     5881 cggcggctga acactggaag gtctatctgg cgacaatgct aatcgccttt ggctcggtcg
     5941 tgcctttcat tatctacgct gaagttaagc gcaaaatgaa gcaagtcttt gtcttctgcg
     6001 ttgggttaat cgtggttgcg gaaattgtgt tgtggaacgc acaaacgcag ttctggcaac
     6061 tggtggtcgg cgtgcagctt ttctttgtag cgtttaattt gatggaagcc cttctgcctt
     6121 cacttatcag taaagagtcg ccagcaggtt acaaaggtac ggcgatgggt gtttactcca
     6181 ccagccagtt tcttggcgtg gcgattggcg gttcgctggg cggctggatt gacggcatgt
     6241 ttgacggtca gggagtattt ctcgctggcg caatgctggc cgcagtgtgg ctggcagtcg
     6301 ccagtaccat gaaagaaccg ccgtatgtca gcagcttgcg cattgaaatc ccggcggaca
     6361 ttgccgcaaa cgaggcgtta aaagtgcgtt tgctggaaac cgcgggcgtc aaagaagtgt
     6421 tgattgcaga agaagaacat tcagcttatg tgaaaatcga cagcaaagtg acgaatcgct
     6481 ttgaggttga acaggcaatt cgtcaggcat aaaaaacgag gcgaattgcc tgctgcgcta
     6541 cgcttaccag gcctacaaag ttcttgagtt acaaatcgtt gtaggccgaa ttagctgttc
     6601 atgctgcatt cggcatgaac aaaacgcgca ttgtcgcgat taatcgcgga agtttttgaa
     6661 ctggaacggc tgaccgagat cgccaccacg taccatcgcc atgacagcct gcaaatcatc
     6721 acgagattta cccgttacgc ggatctcatc gccctgaatt tgcgcctgca ctttcagttt
     6781 gctgtctttg atcattttga cgattttctt ctgagtcgcg ctctcaatgc cctgtttcag
     6841 tttcgcttcc acaaaccagg ttttaccgct atgaacgata ttttccggta catccagtga
     6901 actgccttca atgccgcgct tcagcagctt ggcacgcaga atatccagca actgattgac
     6961 ctggaaatcg gactcgctca acactttgat ggttttgctg gcgtcgttca gctcaaatga
     7021 ggcttcaacg ttacggaagt caaaacggga ctccacttcg cggctagcgt tatcgaccgc
     7081 gttacgtgct tcctgaagat caacttcaga gacaatatcg aaagatggca tcttttcctc
     7141 tcccttcatt tttgatgcga agcataatac ccgcaaagtt aaataaccga cagtcccgca
     7201 ccaggacgta tcccttaatt gtgcatagtc gcaacagcat gcacaattaa gggatagcct
     7261 tattgaacac ccggagtggt tgcgggtgag gaggaacaat gaaaattacc gtattgggat
     7321 gcggtgcctt agggcaatta tggcttacag cactttgtaa acagggtcac gaagttcagg
     7381 gctggctgcg cgtaccgcaa ccttattgta gcgtgaatct ggttgagaca gatggttcga
     7441 tatttaacga gtctctgacc gccaacgatc ccgattttct cgccaccagc gatctgctcc
     7501 tggtgacgct gaaagcatgg caggtttccg atgccgtcaa aagcctcgcg tccacactgc
     7561 ctgtaaccac gccaatactg ttaattcaca acggcatggg caccatcgaa gagttgcaaa
     7621 acattcagca gccattactg atgggcacca ccacccatgc cgctcgccgc gacggcaatg
     7681 tcattattca tgtggcaaac ggtatcacgc atattggtcc ggcacggcaa caggacggcg
     7741 attacagtta tctggcggat attttgcaaa ccgtattgcc tgacgtcgcg tggcataaca
     7801 atattcgcgc cgagctgtgg cgcaagctgg cagtcaactg tgtgattaat ccactgaccg
     7861 ccacctggaa ttgcccgaat ggtgaattac gtcatcatcc gcaagaaatt atgcagatat
     7921 gcgaagaagt cgcggcagtg atcgaacgcg aagggcatca tacttcagca gaagatttgc
     7981 gtgattacgt gatgcaggtg attgatgcca cagcggaaaa tatctcgtcg atgttgcagg
     8041 atatccgcgc gctgcgccac accgaaatcg actatatcaa tggttttctc ttacgccgcg
     8101 cccgcgcgca tgggattgcc gtaccggaaa acacccgcct gtttgaaatg gtaaaaagaa
     8161 aggagagtga atatgagcgc atcggcactg gtttgcctcg cccctggtag tgaagagact
     8221 gaagccgtca ccactatcga cctcttggtt cgcggcggta tcaaagtcac cactgccagc
     8281 gtcgccagcg atggtaacct agcgattacc tgctcgcgcg gcgtgaagct gctggcggat
     8341 gcgccgctgg ttgaagtggc tgatggcgaa tatgacgtga ttgtgctgcc tggtggcatt
     8401 aaaggcgcag agtgttttcg cgatagcacc ctgctgattg aaaccgttaa acagttccac
     8461 cgttccgggc gtatcgtcgc ggctatttgc gccacgccag ccaccgtgct ggtgccgcac
     8521 gatatcttcc cgattggtaa tatgaccggc ttcccgacgc tgaaagacaa aattcccgcc
     8581 gaacaatggc aggacaagcg cgtcgtctgg gatgcacggg taaaattgct gaccagccag
     8641 gggccgggta cagctatcga ctttggtctg aaaattatcg acctgttggt tgggcgtgaa
     8701 aaagcccatg aagtggcatc acaactggtg atggcggcag ggatttataa ttattacgag
     8761 tagtgtcgga tgcggtaagc gtcgcatctg accagatgcg acgttacaac cacaaattac
     8821 gggcgataca ccttcacatt gttaaagccc tgctcgcgca gatagagcgc ctgcaggcgg
     8881 ctcatcaccc cgcgctcaca ccacaacagc caggttttgt tctggtcgag atcgccaaat
     8941 ttggtgctca gtttatagaa cggcagagaa accacatcaa tcccttcgac tttcagtggc
     9001 ttatcttcct gttcatcgac agaacggata tcgagaatca cgtcgttcgg gccgaagtca
     9061 ttgacggttt ccacttccac cacttcctgc tcggcctgct gggcgatttc gcggatatca
     9121 acgttattcg cctcctcaac cactttatcg agaatgctga agtcgaactt ctcttcttcc
     9181 gcttcaatct tcgatttaac cgctttcacc gtcgggcttt tggagatcac gccacaatat
     9241 tccggcatcg tgcgggcaaa gtcttcagtg ccaatctggc gagccaggtt gatgatgtgc
     9301 tctttgtcgt aagaaatcag cggacgcagg atcagcgtat cggagacgtt atcaatcagg
     9361 cgcaggttgg tcagcgtctg gctggacacc tggccgagcg cttcgccggt gaccagcgcc
     9421 tgtacgccgt aacgttctgc cactttggac gcggcacgca ccatcatacg tttgaggata
     9481 acacccatct gaccgtcgtc gattttctcg agaatttccc cgacgacagg ttcgaaatta
     9541 atagcgacaa aacgcacgcg gtgggagctg ccaaagcggt tccacagata atgcgccacc
     9601 tgacgcacgc caatttcatg cgccgcgccg ccgaggttaa agaagcagta atgcacgcgg
     9661 cagccgcgac gcatcaacat ataactggaa acgccggagt cgaaaccacc ggaaatgagc
     9721 gacagcacat cttcctgggt gccgatcggg aaaccgccaa taccttcgta gcggccttta
     9781 atcagcagga gacgatcgtc ttcaacttcc agatggacag tcacatccgg attggtcagc
     9841 ttcacgcgcg cggattcaat atgctgattt aaaccgccgc cgacgtaacg ttccacatca
     9901 atcgagctaa aatcatgttt gccacggcgc ttcacgcgca cgcagaaggt tttgccttcc
     9961 agctgatcgc gatactgaac caacgctttc tcgaaaatat cgtgcatgtc ggtaaacggc
    10021 acgtcttcga cttcgagaat atggtggata cccggaatac gggtcagggc gtcgcgaata
    10081 gccagacgct ggttttcatc ttttgcgcga acttcgatgt tatcccagtg gcggacgaca
    10141 gcgagcgtct catcatagtg ctttaaaacg ttacgaatgt tcccggtaag gatttttata
    10201 aagcgcaagc gcacagattg gcttttgatg gtgatttccg ggaacaattt aatgataaac
    10261 ttcatggcgg caatggttcg ttggcaagcc ttaagcgact tgtataggga aaaatacagc
    10321 agcccacacc tgcggctgca tccaggcgcg gaagtatacc actaacatcg ctttgctgtg
    10381 cacatcacct taccattgcg cgttatttgc tatttgccct gagtccgtta ccatgacggg
    10441 gcgaaaaata ttgagagtca gacattcatt atgccgaaga aaaatgaggc gcccgccagc
    10501 tttgaaaagg cgctgagcga gctggaacag attgtaaccc gtctggaaag tggcgacctg
    10561 ccgctggaag aggcgctgaa cgagttcgaa cgcggcgtgc agctggcacg tcaggggcag
    10621 gccaaattac aacaagccga acagcgcgta caaattctgc tgtctgacaa tgaagacgcc
    10681 tctctaaccc cttttacacc ggacaatgag taatggactt tccgcagcaa ctcgaagcct
    10741 gcgttaagca ggccaaccag gcgctgagcc gttttatcgc cccactgccc tttcagaaca
    10801 ctcccgtggt cgaaaccatg cagtatggcg cattattagg tggtaagcgc ctgcgacctt
    10861 tcctggttta tgccaccggt catatgttcg gcgttagcac aaacacgctg gacgcacccg
    10921 ctgccgccgt agagtgtatc cacgcttact cattaattca tgatgattta ccggcaatgg
    10981 atgatgacga tctgcgtcgc ggtttgccaa cctgccatgt gaaatttggc gaagcaaacg
    11041 cgattctcgc tggcgacgct ttacaaacgc tggcgttctc gattttaagc gatgccgata
    11101 tgccggaagt gtcggaccgc gacagaattt cgatgatttc agaactggcg agcgccagcg
    11161 gtattgccgg aatgtgcggt ggtcaggcat tagatttaga cgcggaaggc aaacacgtac
    11221 ctctggacgc gcttgagcgt attcatcgtc ataaaaccgg cgcattgatt cgcgccgccg
    11281 ttcgccttgg tgcattaagc gccggagata aaggacgtcg tgctctgcca gtactcgaca
    11341 agtacgcaga gagcatcggc cttgccttcc aggttcagga tgacatcctg gatgtggtag
    11401 gagatactgc aacgttggga aaacgccagg gtgccgacca gcaacttggt aaaagtacct
    11461 accctgcact tctgggtctt gagcaagccc ggaagaaagc ccgggatctg atcgacgatg
    11521 cccgtcagtc gctgaaacaa ctgactgaac agtcactcga tacctcggca ctggaagcgc
    11581 tagcggacta catcatccag cgtaataaat aaacaataag tattaatagg cccctgatga
    11641 gttttgatat tgccaaatac ccgaccctgg cactggtcga ctccacccag gagttacgac
    11701 tgttgccgaa agagagttta ccgaaactct gcgacgaact gcgccgctat ttactcgaca
    11761 gcgtgagccg ttccagcggg cacttcgcct ccgggctggg cacggtcgaa ctgaccgtgg
    11821 cgctgcacta tgtctacaac accccgtttg accaattgat ttgggatgtg gggcatcagg
    11881 cttatccgca taaaattttg accggacgcc gcgacaagat cggcaccatc cgtcagaaag
    11941 gtggcctgca cccgttcccg tggcgcggcg aaagcgaata tgacgtatta agcgtcgggc
    12001 attcatcaac ctccatcagt gccggaattg gtattgcggt tgctgccgaa aaagaaggca
    12061 aaaatcgccg caccgtctgt gtcattggcg acggtgcgat taccgctggc atggcgtttg
    12121 aagcgatgaa tcatgcgggc gatatccgtc ctgatatgct ggtggttctc aacgacaatg
    12181 aaatgtcgat ttccgaaaat gttggcgcgc tcaacaacca tctggcgcaa ctgctttccg
    12241 gtaagcttta ctcttcactg cgcgaaggcg ggaaaaaagt tttctctggc gtgcccccca
    12301 ttaaagagct gctcaaacgt accgaagaac atattaaagg catggtagtg cctggcacgt
    12361 tgtttgaaga gctgggcttt aactacatcg gcccggttga cggtcacgat gtgctggggc
    12421 ttatcaccac gcttaagaac atgcgcgacc tgaaaggccc gcagttcctg catatcatga
    12481 ccaaaaaagg tcgtggttat gaaccggcag aaaaagaccc aatcaccttc cacgccgtgc
    12541 ctaaatttga tccctccagc ggttgtttgc cgaaaagtag cggcggttta ccaagctatt
    12601 caaaaatctt tggcgactgg ttgtgcgaaa cggcggcgaa agataacaaa ctgatggcga
    12661 ttactccggc gatgcgtgaa ggttccggta tggtcgagtt ttcacgtaaa ttcccggatc
    12721 gctattttga tgtagcgatt gccgagcaac acgcagtgac ctttgctgcg ggtctggcga
    12781 ttggaggcta caaacccatt gttgcgatct actccacctt cctgcaacgc gcctatgatc
    12841 aggtgctgca tgacgtggcg attcaaaagc ttccggtcct gttcgccatc gaccgtgcgg
    12901 gcattgttgg tgctgacggt caaacccacc agggcgcttt tgatctctct tacctgcgct
    12961 gcataccgga aatggtcatt atgaccccga gcgatgaaaa cgaatgtcgc cagatgctct
    13021 ataccggcta tcactataac gatggcccgt ccgcggtgcg ctacccgcgc ggcaacgcag
    13081 ttggcgtgga actgacgccg ctggaaaaac tgccaattgg caaaggcatt gtgaagcgtc
    13141 gtggcgagaa actggcgatc cttaactttg gtacgctgat gccagaagcg gcgaaagtcg
    13201 ctgaatcgct gaacgctacg ctggtcgata tgcgttttgt gaaaccgctt gatgaagcgt
    13261 tagttctgga aatggccgcc agccatgaag cgctggtcac cgtagaagaa aacgccatta
    13321 tgggcggcgc aggcagcggc gtgaacgaag tgctgatggc ccatcgtaaa ccagtacccg
    13381 tgctgaacat tggcctgcct gacttcttta ttccacaagg aacgcaggaa gaaatgcgcg
    13441 ccgaactcgg cctcgatgcc accggtatgg aagccaaaat caaggcctgg ctggcataat
    13501 ccctactcta ctcctgctat gcttaacaaa ttattcatag actctaaata attcgagttg
    13561 caggaaggcg gcaaacgagt gaagccccag gagcttacat aagtaagtga ctggggtgaa
    13621 cgacaaatcc gcatggagcg gatttgaacg ctgcttgcag cggccctgtt aagggtgagg
    13681 cccatggatg ggccgaataa ctcgcagccg cagcacatgc aacttgaagt atgacgagta
    13741 tggcaggagt ggcagcatgc aatacaaccc cttaggaaaa accgaccttc gtgtttcccg
    13801 actttgtctc ggctgtatga cctttggcga gccagatcgc ggtaatcacg catggacact
    13861 gccggaagag agcagccgtc ccatcattaa acgcgcactg gaaggcggca taaatttctt
    13921 tgataccgcc aatagctatt ccgatggcag cagcgaagag atcgtcggtc gcgcactgcg
    13981 ggatttcgcc cgtcgtgaag acgtggtcgt tgcgaccaaa gtgttccatc gcgttggtga
    14041 tttaccggaa ggattatccc gtgcacaaat tctacgttct attgacgaca gcctgcgccg
    14101 tctcggcatg gattatgttg atatcctgca aattcatcgc tgggattaca acacaccgat
    14161 cgaagagacg ctggaagccc tcaacgacgt ggtaaaagcc gggaaagcgc gttatatcgg
    14221 cgcgtcatca atgcacgctt cgcagtttgc tcaggcactg gaactccaaa aacagcacgg
    14281 ctgggcgcag tttgtcagta tgcaggatca ctacaatctg atttatcgcg aagaagagcg
    14341 cgagatgctg cctctgtgtt atcaggaggg cgtggcggtg attccatgga gcccgttggc
    14401 gcggggccgt ctgacgcgtc cgtggggaga aaccaccgca cgactggtgt ctgatgaggt
    14461 ggggaaaaat ctctataaag aaagcgatga aaatgacgcg cagatcgcag agcggttaac
    14521 gggcgtcagt gaagaactgg gggcgacacg agcacaagtt gcgctggcct ggttgttaag
    14581 taaaccgggc attgccgcac cgattatcgg tacatcgcgg gaagaacagc ttgatgagct
    14641 actgaacgcg gtggatatca ctttgaagcc ggaacagatt gctgagctgg aaacgccgta
    14701 taaaccgcat ccggtcgtag gatttaaata ggaatccagg cctgatgaga cgcaacaagc
    14761 gtcacatcag gcactggtgc acaactacga cagaataccc agcggccagt gatgaccgat
    14821 aaagtacagg atgcctgcgg aaatcacccc ggcgacaata tcgtcaatca tgatccccat
    14881 gccgccatgt acattgcgat caaaccagcg gatcggccac ggtttccaca tatccaggat
    14941 acggaaaatc acaaacccgg cggcaaccca ctgccagtca ttggtcggca gcgccatgag
    15001 cgtgatccac ataccgataa attcgtccca gacaatgctg ccatgatcgt gcacgcccat
    15061 gtctttcgcc gtttgatgac aaagatagac gccgatacag atccccagca ttaccaccag
    15121 cgaatagagc tgccagggca aaaaagtcat cagataccag aagggaatcg ccgccagcga
    15181 ccccatcgtg ccaggaacga tcgggcttaa tccacttccg aatccgacag caagcagatg
    15241 ccacggatta ctcattttca ggcgactttt cgcgacatct ttatggcgtg gcaaaatggt
    15301 catatccttt ccagtctaac gtgacaggtt cgccgtcacg aataaaacaa agcccttcga
    15361 tatcggcggt catttgcccg atacaggtaa acggtacgcc caggtgtccg agagccacat
    15421 ccagcgcgcc acggttcagt tccggcaccg tgaaacacaa ctcgtaatct tcaccgccag
    15481 agagcgccca gcgcagcgcc tgttccggtt caacatggcg agaaagcgca tcagaaaacg
    15541 gcagcaatgc caggtcaata cgtgcgccgc agtcgctggc tttcacgata tgcccgagat
    15601 cggaaatcag accgtcagag agatcgatag ctgaatttgc cagatcgcgc agtgcctgcc
    15661 cctgtaaaat acgcggcgac ggacggagat gacgtttgat caagtagtcc gcatctttag
    15721 catcggcaac ctgcaaacgg ttttgcaaaa tcgccagccc ggcagcgcta tcgcccggtg
    15781 taccggtcac atagatccag tcacccggtt tcgccccaga gcgcgttaag gctcgtccca
    15841 tcggaacaaa gccgtggata cccaacgtca ttgataatgg cccacgcgtg gtatcgccgc
    15901 caatgagttg catatcgtaa taattgagaa gatcaaacaa gctgtcgctg aaggactcaa
    15961 gccacgcttc gtcgacgtcc ggtaaggtta atgccagcgt cagccaggcc ggatcggccc
    16021 ccatcgctgc cagatcgctt aggttcaccg ccagtgcttt ataagccaga tcagcaggat
    16081 cgatatcagg gaggaagtgg ttgcccgcca ccagcgtatc agtgctgatc gccagggtct
    16141 gtttctcagg gatattgaga agagcgcaat cgtcgccgat gcccagttcg acatcaagac
    16201 gagaacttct tacacggtca aaataacggg caatcaggga gaactcgcca catgccatac
    16261 gttatgcctc agcaggtaaa aaagaaaagg ccggcgatca tggaacggtc ttccgtgaat
    16321 ctaccggcct ggatattact ttttgttagg gcgaatcaca ggtgctgctt tatcgagtac
    16381 gccgttgacg aacttatggc tgtcttctgc gccgaaagat ttcgccagtt cgatcgcttc
    16441 gttaatggcc actttgtatg gcacatcgct acgtttagac agctcgtaca gcgcaatgcg
    16501 cagtactgct ttttctacct ggcccagttc ttccagcaga cgggacaggt acggcttcat
    16561 cagtccgtcg aggtatgcgg tgttagtcgc caccccggcc agcagctcac ggaagtacag
    16621 gacgtcaacg tcttttacat cctgttcagc caggaactgg tattcaacat cagcgatgtc
    16681 gttctgggac aactgccagg agtagagcgc ctggacggca cactcacgag cgcggcgacg
    16741 agcagcaggt ttcacggatt tccccttact aatttcaggc cttgatggct ttcaatacat
    16801 taatcatttc aagcgcggtc agtgcagctt ctgcaccttt gttgccagct ttggtgccag
    16861 cacgttcgat cgcttgttca atgctttcag tggtcagaac accaaaagca accggaattt
    16921 cgctgtcctg ggcaacatgc gccaggccgt tgcttgcacc accagcgaca tattcaaagt
    16981 gggcagtgcc accacgaata accgtaccca gcgcaatcac cgcgtcgtat ttaccggttt
    17041 tagccagtgc acccgccgcc agcggcagct cataggcacc tggcacccaa acaacggtaa
    17101 tgttttcatc ttttacctga ccgatacgtt tcagtgcgtc aattgcacct tccagcaggc
    17161 tgtcattgat aaagttgttg aaacgcgcga tggtgatggc gacgcgagcg tccggggtag
    17221 caacgttagc ttcaataatg ttcattttct tccttcgggt tcgagtatgg ccccgcaggg
    17281 gggcggattt tagcataata tttcgtgcgc tgcttccctt tcgagccggg agatcatgca
    17341 cccactaaat gcaggcaaac atccgggcct acatgacgta tctctttgaa tttaaattgg
    17401 ggggcgtcgg ctaatttctc aagtcctggc atcgtgcata atccacgggc gtcgttgcct
    17461 aatagtttag gtgcgatata gacaatcagc tcatcaacta aaccagcctg tagcaatgcg
    17521 ccagcgagcg ttggccccgc ttccacccag atgctgttaa tttgctgttt acccagttgc
    17581 atcatcagta caaccagatc cagatgacct ttatgctctg gaatcagcaa ggtacgcacc
    17641 gtttccggcc actcacgaga atcttcctgc gtacgcgcga accaggtttc gccgggctgc
    17701 tgcacaatgc gatgttccgg cgtcacgcga ttttggctat caatcacaat acgtatcggc
    17761 tgacggagat tttgttgcgg ataaagcgcc tgagtttgtt catccagttc agaccaacgc
    17821 actgttaagg ctggatcatc cgccagcacc gtggcgctgc tggttaaaat ggcatgactt
    17881 tgtgcgcgca gtcgttgtac atcgagtcgc gcctgaggcg aagtgatcca ctggctttcg
    17941 ccactcgcca tcgccgtgcg accatcaagc gatgcgccaa gttttaattg aatataagga
    18001 aagccggtgc gcatccgttt gagaaagcct ttattcaatt gctcggcttc actcatcatc
    18061 agcccgtggc tgacgtcaat gccagcctgt tgcagacgat atagccctcg cccagctacc
    18121 tgcgggttag gatcttgcat cgaggcaacc acgcgcgcca cacctgcggc aattaatgca
    18181 tcacagcaag gcggcgtacg gccgtgatgg ctgcaaggtt cgagtgtcac atacgcagtc
    18241 gcacctttgg ctttttctcc agccatacgc aacgcatgta cttcggcatg tggttcacca
    18301 gcacggtgat ggtaaccttc accgacaatt tcgccatctt tgacaatgac gcacccgaca
    18361 ttcgggttgg gatgcgtggt aaaacgtcct cgttgcgcca gctttagcgc ccgcgccatg
    18421 taatactcgt cctgcacggc ttagtcctcc aggcgcgcga tctcttcgcc aaattctttg
    18481 atatcttcga aactgcggta gacagaggca aaacggatat aggcgacttt atcaagcttt
    18541 ttcaattgct ccatcaccag attgccaatc atcttgctcg gcacttcgcg ctcaccggtg
    18601 gcgcgcagct gcgatttaat atgattgatt gccatttcga cgtcatcgga actcaccgga
    18661 cgtttttcca gcgcccgcag cattccgcta cgtaatttct cttcattaaa cggttcacgc
    18721 acgtcgttgc tttttacaac acgcggcata accagctccg ccacttcaaa ggtggtgaaa
    18781 cgttcattac acaccagaca ctgccgacgg cggcgtacag atgagccctc gcccacgaga
    18841 cgagagtcaa ttaccttagt gtccacggcg aaacagaatg ggcaatgcat acggcgtcct
    18901 gaccaggtgg ttaacagaaa tctattttac cctgaactga cgatacaaca aaggcgcaac
    18961 gcttttgaga taaaggatcg atgctttcgc tgcttttacc ctctaccatt aggctaattg
    19021 cgacgagaaa gggacgacct atgaatacta acgtttttcg actgctcctg ctgggaagcc
    19081 tgttcagcct tagcgcctgt gtgcagcaaa gcgaagtgcg acagatgaaa cacagcgtca
    19141 gcacgctgaa ccaggagatg acgcaactca ataaagaaac ggtcaaaatc actcagcaaa
    19201 acaggctgaa tgcaaaatcc agcagtgggg tttaccttct gcctggagcg aaaacaccag
    19261 caagactgga aagccagatc ggtactttac gtatgtcgct ggtgaatatt acgcctgata
    19321 cagatggcac caccctgacg ctacgtattc agggcgagtc gaatgatccc ttgcccgcat
    19381 tcagcggaac tgttgaatat gggcaaattc agggaacaat agacaacttt caggaaatca
    19441 atgtgcagaa tcaattgatt aacgcccctg ccagcgtcct cgcccccagc gatgttgata
    19501 ttccgttaca gttaaagggt atgtctgtag atcagttagg ctttgtacgt atccacgaca
    19561 ttcaacctgt tatgcagtaa acgtattgcg gggcgatttt gtgcgtcccg caacatcttt
    19621 tcccgtcatt ttgttactcc gcttacatca cctggattga tagtaaaagt ttgcaacaag
    19681 ggtgaaagtc agtacaatcc ccgcccgaat gtgtgtaaac gtgaacgcaa tcgattacgt
    19741 aaatgataga actgtgaaac gaaacatatt tttgtgagca atgattttta taataggctc
    19801 ctctgtatac gaaatattta gaaacgcaat ttgcgccttt ttcactcccg caagggattt
    19861 tcaaacagtg gcatacatat gaaaaaaaca ttactggcag ccggtgcggt actggcgctc
    19921 tcttcgtctt ttactgtcaa cgcagctgaa aacgacaaac cgcagtatct ttccgactgg
    19981 tggcaccaga gcgttaacgt tgtcggaagc tatcacaccc gtttcggacc gcagatccgc
    20041 aacgatacct accttgagta cgaagcattc gctaaaaaag actggttcga cttctatggt
    20101 tatgcggatg cgccggtatt cttcggcggt aactccgatg caaaaggtat ctggaaccac
    20161 ggttctccgc tgtttatgga aatcgaacca cgtttctcca tcgacaagct gaccaatact
    20221 gaccttagct ttggtccgtt caaagagtgg tacttcgcga acaactacat ttacgacatg
    20281 ggtcgtaata aagatggtcg ccagagcacc tggtacatgg gtctgggtac cgatatcgat
    20341 actggcctgc cgatgagcct gtccatgaac gtctatgcga aataccagtg gcagaactat
    20401 ggcgcagcga atgaaaacga gtgggacggt taccgtttca aaattaaata ctttgtgccg
    20461 attaccgatc tgtggggcgg tcagctgagc tacatcggct ttaccaactt cgactggggt
    20521 tccgatttag gggatgacag cggtaatgca atcaacggta ttaagacccg taccaataac
    20581 tccatcgctt ccagccatat tctggctctg aactacgatc actggcacta ctctgtcgta
    20641 gctcgttact ggcacgacgg tggtcagtgg aacgacgatg cagaactgaa cttcggcaac
    20701 ggcaacttca acgttcgctc taccggctgg ggtggttacc tggtagtagg ttacaacttc
    20761 tgatgaatga aatgccggat cttgtatccg gcatttcagt ttgttaacaa cgtgcgcttt
    20821 gttcatgccg gatgcggcgt aaacgcctta tccggcctac aaaagcacgt aaattcaata
    20881 cattgctgag attacgttgg cctgataagc gtagcgcatc aggcaatttt gcgtttttat
    20941 cacttcttct tgttcatcat cgctttcagg tcagcgaatg ggttgtactt cgcttcaccg
    21001 acatctttct gcgcatcttc ccctgcgata acggtcgtac catactgatc cgcttcggtg
    21061 tatttcgaat gctcatgatc gtggcaatag agacaaagta attcccagtt actgccatct
    21121 tccgggttat tagtatggtc gtgatcaatg tggtgaacgg taagttcacg caggttggaa
    21181 taaacaaact cgcgggaaca gcgaccgcag acccacggat agatttttaa tgctttttcg
    21241 cgatagtcgc tttctaaccg cgcatagttt tttgggatga tagccatgaa aaagttacct
    21301 ggatgaaatg tagggttacg cgcagtttcc caagatgcgg ggattttcgc aatcacctta
    21361 catcaaaaaa atcccgatct tctgaccggg attttcaaca tcagctgaaa cttgattaag
    21421 gcagaattga cggctgatcc gccccttctt tttccacttt ctgctgcaac atgtgttcgc
    21481 gcttcatacc cagtttcaga gccagcgcag atgccacata gatggaagat gcagtaccga
    21541 tggaaacacc gataagcatg gtcagcgaga agccttccag taccggacca ccgaagaggt
    21601 acagcatcag gataaccatc aaggtagtac cggatgtgat caaggtacgg tgcagcgtct
    21661 gggtcaagga cacgttaaag atttcgtaag gcgtaccgcg acggatcttg cggaagtttt
    21721 cacgaatacg gtccgatacc acgatactgt cgttaagcga gtaaccgata actgacatca
    21781 acgatgccac aatggtcagg tcaatctcga tatggaataa cgacaaaata cccagcgtaa
    21841 tgataacgtc gtgcgccagc gcaataacaa cccctgccgc cagtcgccac tcaaagcgga
    21901 aacctacgta cacgaggata gacagcagcg ccgccatcaa cgccatcgca ccggtttgcg
    21961 caaggtctgc ccccacgctc ggaccgacga actcaatacg cttcactgcc gcattctgat
    22021 tggtggattc gttaatcacc ttcagaacct ggctgcccag cacctgaccg ccggtttcgc
    22081 cttcagcagg cggcatacgg accatgatgt cgtggctgct accaaagttt tgcagcatcg
    22141 gctcttcaaa accggctttt tgcaatgcat cacgcattac gtcaatttca gccggttttt
    22201 cgagcgtaat ttcgataacc gtaccaccgg tgaaatccag cccccagtta aagccgcgca
    22261 cgcccataat aacgatagcg gcgattaaca gcagaccaga gatgccaaaa gcccagtagt
    22321 cccagcgcat aaagtcatag actttacggc cgtggtttag ttgttcaaca gtatattcct
    22381 gtgccacatc gcactcctca gattgacagc ttcttgacgc gcttgccgcc atatagcagg
    22441 tttacgatgg cacgcgtacc gacaatcgcg gtaaacatcg acgtcgccac accgataccg
    22501 gtagtaatcg cgaacccttt aattgccccg gtacccactg cgtacaggat gatgacttta
    22561 atcagcgtgg tgatgttcgc atcgaagata gaactgaatg cgccacgata accttcatca
    22621 attgcctgtt gaacagtacg tccgttgctc aactcttctt taatacgttc gttgatcagt
    22681 acgttcgcat cgaccgctac cgcaagggtt aagacgatac ccgcgatacc tggcatactc
    22741 agcgttgcgc ctggcaacag cgacataata ccgacaatca agatcaagtt ggcaatcaga
    22801 gcactggtcg caatcagacc aaacttctta tagaagatga tcatgaacag aatagaaacc
    22861 agcagaccgg caaggcaagc ctccagcccc tgctcaatgt tctgcatacc cagggttggg
    22921 ccaatggtac gttcttcaac aatctgaatc ggcgcgatca acgcaccggc acgcagcagc
    22981 agtgaaagct gacgcgcttc gcttgggtta ttgatgccag taatacggaa gctgttaccc
    23041 agacgagact ggatgttggc gatattaatc acctcttcct gtttcaccag aaccgcacga
    23101 ccgtttgcat ctttcttacc gctgtctttg tactccacaa acagggttgc catcggtttg
    23161 ccgatgttgt ccttggtgaa gttagacatg atgttaccac ctgcgctgtc gagcgagatg
    23221 ttaacctgcg gctggttgta ttcgtcctgg ctggaagtgg agtcggtgat atggtcaccg
    23281 gtcagaatta cgcgtttgta cagcacaacc ggctgacctt cgcgggtctg tttcacttca
    23341 gagtcgcccg gtacgcgacc ggatgccgca gcggcctgat caacgttggt gtttaccaga
    23401 cggaattcca gcgttgccgt cgcacccaga atctctttcg cacgcgcagt gtcctgaata
    23461 cctggcagtt caacaacgat acggtcagca ccctgacgct gaaccaccgg ctccgccacg
    23521 ccaagttggt ttacacggtt acgcaggata ttaatgttct gctgcaccgc atattcacgc
    23581 gcttcactca ggcgagcatc gctcattacc gcacgcagct ggttgctgcc ctggctgcta
    23641 atcaccaggt ccggatggcg cttgctcaga tacgcaatgg cttcatcacg agctttagca
    23701 tcgcggaaag tgatgctcag gccgtagttg ttttctttac gaacggtggt atacgggatg
    23761 cccttttcgc gcaggtcgct gcgcaggcta tcgatgtttt gttcctggag tttgccaagc
    23821 gcggtgtcca tatccacttc catcaggaag tgaacgccgc cacgcaggtc aaggccgagc
    23881 ttcatcggct cagcgtgaat agctgccagc cagcgcggcg ttgccggggc aaggttaagc
    23941 gccacgacgt atttgtcacc cataacgccc attaatgctt cacgagcgcg cagctgggtg
    24001 tcagtggagt cgaagcgcgc aagaatagcg ccctcttcca gtgccacaga cttagcagtt
    24061 attttttctt cttgtaacgt tttctggacc tggatcagcg tttgctcact ggcggcgact
    24121 ccgcgcgcac cagtgatctg aacagccgga tcctcaccaa acaggttggg aagcgcatac
    24181 agcagaccga tgacaatcac cacgatcagc atgacgtact tccacaaagg ataacggttt
    24241 aacacggcaa ttcccttagg gaaaaatttt aattacagcg ccttcatggt gcctttcggc
    24301 aggacggcag ctacgaagtc acgtttaata actacttcag tggtgtcatt cagcgcgata
    24361 gcaatgtagc cgttttccgc tactttggtt acgcgaccaa ccaggccacc gttcgtcaga
    24421 acttcatcac ctttggcaat ggagtccatc agctttttgt gttctttggt gcgcttctgc
    24481 tgtggacgca ggatcatgaa atagaaaatc agaccgaata ccaccagcat caaaatcaaa
    24541 gacatcgggc taccttgcgc cggtgcaccc gttgccgcta ccgcatcaga aataaaaaag
    24601 ctcattaaat ttccctcatt attaatatta atcaacgttc aaaggtggta cttctcgccc
    24661 ctgacgctgg taaaaatcag ttacgaagct ctctaattta ccctcttcaa tagccttgcg
    24721 taaacccgcc atcaaacgct ggtagtaacg aaggttatga atggtattga gtcgcgcgcc
    24781 taatatttcg ttgcaacggt cgagatgatg caagtaagcg cgtgaataat tgcgacaggt
    24841 gtagcaatca cactcaggat cgagtgggcc agtatcgctc ttatacttcg cattgcggat
    24901 tttcaccacg ccatcggtca cgaacaaatg accatttcgg gcgttgcggg ttggcattac
    24961 gcagtcaaac atatcgatac cgcgacgtac gccctcaacc aggtcttctg gtttaccaac
    25021 gcccatcagg taacgcggtt tgtctgccgg aatttgcggg catacatgct ccagaatgcg
    25081 gtgcatatct gctttcggct cacccacagc cagaccgccg acagcgtagc catcaaaacc
    25141 gatatctacc agacctttaa cagaaatatc acgtaaatct tcgtaaacac tgccctgaat
    25201 gataccaaac agcgcatttt tgtttccgag actgtcaaaa cgctcacggc tacgcttcgc
    25261 ccaacgcaga gacatctcca tggagcgttt tgcgtaatcc cagtcagcag gatacggcgt
    25321 acactcatca aagatcatga cgatatccga accaagatcg tactgaatct ccatcgactt
    25381 ttccgggtcg aggaaaatcg ggtcgccgtt gatcgggtta cggaagtgca cgccctgttc
    25441 ggtgatttta cgaatatcac caaggctgaa gacctggaag ccgccggagt cggtaagaat
    25501 cggccctttc cactgcataa aatcgtgtaa atcgccatgc agtttcatga tttcctggcc
    25561 cgggcgcagc cacaggtgga aggtgttacc gagaataatt tgtgcgccag tggcttcaac
    25621 ttcttccggc gtcatccctt ttacggtgcc gtaggtgcca acaggcataa aacaaggcgt
    25681 ttccactacg ccacgatcaa agaccaggcg gccacggcgt gcgcgaccgt cggtggtgtc
    25741 cagttcaaat ttcatttttt ctccactacg tcagaaaaac agtccaacgt tttaaaccag
    25801 cgccgcggaa ttactccccg acgcgctcat taattgcctg cggattgtac gtgataaaca
    25861 tcgcatcacc gtaactaaaa aagcgatatt tctcttctac cgctgattta taggcgttca
    25921 tggtgtgttg ataaccggca aaggccgaaa ccagcataat cagcgtcgac tctggcaagt
    25981 ggaagttcgt caccagcgca tcgaccactt tgtactgaaa gcccggatag ataaagattt
    26041 gggtatcgtc aaaaaacggt tcaatgagat cgtttttcgc tgcctgagcc gcactttcca
    26101 gcgaacgtac tgaggtggtg ccaaccgcaa tcacccggtt accgcgcgct ttcgccgcca
    26161 gtaccgcgtc tactacatcc tgcggcactt cagcgtattc cgagtgcatg atgtgatctt
    26221 caatggtgtc gacgcgcacc ggctggaagg tgcccgcacc aacgtgcaac gtcacaaacg
    26281 ccatctccac gcctttggcg cgtaattttt ccagcaaagg ctcgtcaaaa tgcagaccag
    26341 cggtcggggc cgcaaccgcg cccggttttt cgctataaac ggtttgataa agttcgcggt
    26401 cagcgtcttc gtccggacgg tcgatatacg gcggcagcgg catatggccg atgctgttga
    26461 ggatatccag taccgaacga tcatcattaa attcaacttc aaacagtgcg ccgtggcgcg
    26521 cggtcattgt tgcgttgata ctttcgtcat cgcccagtaa cagttctgcg ccaggttttg
    26581 gcgctttcga ggcgcgaata tgcgcaagaa tgcgtttgtc gtcgagcatc cgctcaacca
    26641 gcacttcaat cttgccgccg ctggctttac gcccaaacag gcgcgccggg atcacgcggg
    26701 tattattaaa aaccagaaga tcgccggggt tgagcttatc gagtaaatcg gtgaaagtac
    26761 cgtgcgtcag cgcgcccgtc ggcccgtcca gcgacagtaa acgacagcta ctgcgttcag
    26821 gcatgggata gtgggcaatc agggattcgg gcaattcaaa agagaaatcg gtaacgcgca
    26881 tgacactgac tcagactaaa ataagaggcg ggtagtctag tgccggggcg ctctccctgc
    26941 aacctttacc ctccttgatg gatgacataa tctatacaca aaatcattca agatgcatcg
    27001 agatggcaac tgaatgagtc cccaggagct tacattaagt aagtgactgg ggtgagtgac
    27061 aaatctgtcc ggaacagatt tgaacaccgt gtaacggtgg cccgtaaggg cgaggctcat
    27121 ggatgagccg agtaactagc agccaacgaa gaggcagttt gaaggatgaa gtgtatatga
    27181 attttttagc tcacctgcat ttagcccatc tcgcggaaag ctcgctttcc ggcaatttac
    27241 tggctgattt cgtacgcgga aatcctgaag aaagttttcc gcccgacgtc gtggctggca
    27301 ttcatatgca tcggcgaatc gacgtattaa ccgacaatct gccagaagtc cgcgaagcgc
    27361 gggagtggtt tcgtaatgaa acgcgccgtg tcgcgcctat tacgctggat gtgatgtggg
    27421 atcactttct gtcccgccac tggtcgcagc tgtcgcccga ttttccgcta caggaattta
    27481 cctgttatgc ccgggagcaa gtgatgacta ttcttccaga ctcaccgcca cgctttatca
    27541 atctgaacaa ctacttatgg tcagagcagt ggttggtgcg ctatcgcgat atggatttca
    27601 tccagagcgt gttaaacggc atggcaagcc gtcgcccacg tctggatgcc ctgcgtaact
    27661 cctggtacga tttagacgcc cattacgatg ctctcgaaac ccgcttctgg cagttttatc
    27721 cgcggatgat ggagcaggcg tcacgcaagg cgttataaga cgttaattca tccacaccgt
    27781 agccgaaatg gcaggcaaag ccagaatccc gtccgtcagt tgcccgtgac cttctttgcg
    27841 ttgccattgc gcgacattaa gcaacggcga cgcgggtagc accacttcac aggcttcgcc
    27901 acggttgatt gccaccagta cacgctgctg atacagcacg cggacaaata ccaccacgtt
    27961 atcttccgca tacagtacct gacagccgcc acgacgcagc gcctgacttt tcttacgcag
    28021 cgcaatcatt cgctggtaca gcgcgaataa cgccgtatcc tgcttttcca cctgccaggg
    28081 gaacggttta cggcaaaacg gatcgttttt accatccagt cccacttcat caccgtaata
    28141 aatgcacggt acaccaggcc aggtgaacaa ccagaccacc gccagcggca ggcgcgcaat
    28201 atcccgaccg agcagcgttt taaatcgcgc agtatcgtgg ctatcgagct gattaaacat
    28261 acgcagttgc tgttgatgag aaagtccggc gcggtaatta tccatccagg ccatacaggt
    28321 ttgggcgtca atttgctgcg gatcgtaaga gatgtcggta ttggcaagaa atccccacaa
    28381 cgggaatgtg aagccgcgat agttcatggc ggcatcttcc acatcggcct gtaaccattg
    28441 ccgtgcatcg ccaaaatgct ctccaacgat atacgcttcc ggctgggttt ctttcgccgc
    28501 ttcggtgatc ccggctacgt gctgcaaatt attgcgcgcc ccacccgctt cccccagcat
    28561 atgcaccaca tccagccgcc agccgtccat attccacggc gctttcagcc agtggcggac
    28621 gatactgtct tccccacgat aaatttcatt caccagactt tccgactgat aatccagttt
    28681 cggcaagctg gcatagccca gccagtcgag cgccgtgcca tcgtcactaa acgagtacca
    28741 gtcgcgccag ggcgattcgg ggttgtgaca agcaccaccc gtcccgcgat tgtgtctgtc
    28801 aaaccaggca tgggaatcgc cactgtggtt aaacacgccg tccagcacca gccgcattcc
    28861 cagctgctgc gtattgtgtc gcaaacgcaa caacgcccca tcaccgccaa actgcggatc
    28921 gacatggcga taatcctcgg tatcgtattt atgtacgctg ggagctttaa acaccggatt
    28981 gagatacagc gccgtcacgc cgagcttttt cagatatggc agtttttcgc tgataccgtc
    29041 cagatcgccg ccatagaacg ttgatccgcc cgcctgcgcc gtgaccggtt catcccagtc
    29101 acgcaagatg atctcttgtc cggctgcgtg atggtaataa acatgatcct gttcagcttc
    29161 acgaggaaga ctacgggcga aacgatcagg gaagatctga taaaaaatct gatccgcagc
    29221 ccattgtggg gcgatatccg gtacatcgac ggcaaactgc tccagtcgtg ccggcggcat
    29281 tcggctgaag ccctgcggtg taaaccaacg ctggcgatcg tgccacagta atttgaaact
    29341 ataacgccgc cgaggtagtc cgctggagag atcgatcgcc gcccgccatg cggtaacgcc
    29401 aggctgcggc tgactgcgct gcttatgcat cggtactgac gtttcttcgt tgtcgtgttc
    29461 tgtacgcagc ataatgcgct gcggtgggtc ttcgcccgtc agccacagtc taatgagcag
    29521 ttgatctttg ctttgtttaa caaatggggg caccggcagg tgccatgcat ttaacatcat
    29581 aattcccctg tgatgaaaat ctgatcacct tgccatagcc taatgcagga tcactcatca
    29641 tctgaaggat atttggggga ggagggcggg ggcggataac tttcggaata gccggatcag
    29701 gatttcctcc tgaaccggct tacagaacgc aaggattact ggttttcagc cagctgacga
    29761 tcgtggcgac gcttaaacat ccagccaatc aacagcacaa caatccacgc gaaaccgaca
    29821 tacagcgaga tacgcgtatc cgggtgataa ccaatcaacc cgataataaa gagcaggaaa
    29881 atcagcccgc cgatggtggt tgctacccca cccggtactt taaatttcag cgccttaact
    29941 tcttctggcg gcaaacggcg acggaaggca atttgcgaca gcaggatcat aatccacacc
    30001 cataccgtgg cgaaggttgc cagcgaagcg atcaccagga agacgttttc cggcatgatg
    30061 tagttcagat acaccgcaaa cagcagcgcg gtagtcatca ccagcaccgt aacccacgga
    30121 ataccgcgac gcgacgtttt gctgaaaact ttcggcgcgc tgccctgctc tgccataccg
    30181 tggagcatac ggcctacgcc aaatacgtca ctgttaattg ccgaaagcga agcggtcagc
    30241 acaacaaagt taagaatgct ggcggcaaag gtaatcccca tatgctggaa cgtcagtacg
    30301 aacgggctac cggcagtgcc aacctgattc cacgggtaga tagacataat gacgaacagc
    30361 gtaccgacgt agaacaccag aatacgcatc ggcacggagt taatcgcgcg cggaatagat
    30421 ttctcaggat ctttcgcttc accggcggta atcccgataa tttcgatccc accgtaagca
    30481 aacatcacca tttgcaacga catcaccata ccaagccagc cgttactgaa gaagccgccg
    30541 ttgctccaca ggttatgaat accggtcggt tgcccgccgt tgccaatccc ccagatgatg
    30601 atgccgaaac cggcgacaat catgatgatg atggtggcga ctttaaagaa cgagaaccag
    30661 aactccagct caccgaatac cttcacgctc atcaggttta cggcgcaaat gatcagcacc
    30721 acgctcagta cccaaatcca gtgcggcacc gtcgggaacc agacccccat atagatacca
    30781 aaagcggtca catcggcgat ggcgacaata aggatttcaa agcagtaggt ccagccggta
    30841 atgtaaccag ccagcgggcc gaggttttcc tgcgcataac gcgagaaaga gctggcggcc
    30901 gggttatgta ccgacatttc ccccagcgcg cgcataatga tatacgccgc gacaccaccg
    30961 ataatatagg ccaacaacac gctcggaccg gccattttga tggcgtctgc cgaaccgtaa
    31021 aacaacccgg tgccaattgc tgaacccagt gccataaagc gtatgtggcg ggtacttagc
    31081 ccacgcttta gcttgttctt actttccatc aattcctaac ccatcaacac aataaaaaac
    31141 cacggggcat cgcagccccg tggttaaaac aaatgttcag tgatttagtg agcgctggag
    31201 gtcacctgac gacctgccgc gcgatcccag ataatggcca gaaccaccat caccactgtt
    31261 ggcattaacc acgccagacc ttgttcggcc agcggtaaac gctgcgccca ggacggtaag
    31321 atatcgctga atgcagatgc tttgatcccg tcgagaatac caaaaagcag gctgataaac
    31381 atcggcggag caatcacgcg ggacgaatta tgccaccatg agcgtgtaaa acttaataca
    31441 accagtgcga tacacggcgg ataaatagcg gtcagcaccg gtacggagat ctgaatcagc
    31501 tggctcaaac cgaggttaga caccaccatc gagaagccgc cgaggataaa caccagcgta
    31561 cgataagaga gcggtacgta ctgggcaaag aattctgcac aagcacaggt caggccaact
    31621 gccgtgacca ggcaggcgat gaagattaac gccgccagca ggaagctacc gccgccgcca
    31681 aaggtgtgct gaacgtaagc atgcagaata gcagcgccgt ttgcagactg atcgaccagc
    31741 gacgcgctgt ctgaacccag acggaacagt gccaggtaca gcagagtaag accaacaccc
    31801 gccatcagac cagcccagac ggtataacgg gtcagcagac gcgcttcagt aacgccacga
    31861 gaacgcgccg cgttaacaat aacgatacca aacaccatcg cgcccagcgt atccatggtc
    31921 agatagccgt tgacgaagcc gttagaaaac gcagcgtttt gataagcctc agtcgccgtg
    31981 ctgatagaac ccgccggcca gacaatcgcg gcaacagaca ggatgaccag cgcgataatt
    32041 ttcagcggcg caaggaagtt gcccacggta tccagcagct tgcccggata gagcgaaacc
    32101 agaataacga tagcgaaata gaccaggctg tagataaaca gcggcagtgc ggaatcaccc
    32161 gtcagcggcg caatccccac ttcaaaagaa acggtagctg tacgcggcgt agcgaacagc
    32221 ggccccaccg ccaggtaaca aaccgttgcc agcagtacgc cagcaacttt accgattggc
    32281 gtgctgaggc tgtcaacacc gccgccaact tttgccagcg ccaccaccgt taataccggc
    32341 aggccaacgg cagtaatgag gaagccgaat gccgcagtcc agacgtgttc gcctgcctgc
    32401 aagccgacca ttggagggaa aataatgtta cctgcgccga cgaacaacgc aaatgtcata
    32461 aagcccagag cgatgatatc gcgcgatctt aattgatggg tcataaatca tactgcctgt
    32521 ggatgtggtg ttgtaacgtt aaatttttgt gccaatccct ggggatgata cagcggtata
    32581 tttaaacaat tttagcggga aatactccca accaggcgcg gcgaagaata gtttttcgat
    32641 ttaccgcgtt aatgcagtca gtatgacagc acctgaagcg caatttaaac gcttataacg
    32701 ttttaaagca aggagggatc taaaaaccag aggtatatag cgataagaaa tcaccaacca
    32761 gtataaaaca ctatttccgt gaaaaagaca tggctaatca tgcgaacaaa aaatcaccaa
    32821 ccgttgtaaa atctaacgcc cgtattttga ctgcaaagaa aataagccag catcggctgg
    32881 cttatggcaa taaaagatga caaaggcgga ttaatcgctg tttttggcaa ttaaacgttc
    32941 cgggataaca aaactgaaac gtgttccttt tcctactgta ctctcaatat gcaggcgact
    33001 ttcgtgatga ttcaccgcat gtttcacgat cgctaaccct aatccgctac cgccggtttg
    33061 ccgggaacgc gctttatcaa cgcgataaaa acgctcggtc aggcgcggaa tatgctccgg
    33121 tgcgatgccc ggtccattat cttcaacgct aaattcggca ccgtgcggca ctcgctgcca
    33181 gcgtacggtg atatgcgtgc cttccggcgt atgatttacg gcgttataga ccaggttcga
    33241 aatcgcactg cgtagctgat cttcgttgcc agacaccttg aggccgttat ctatctcaaa
    33301 ggtaaatgtc tgttttttct gactcagagt ctgagcctcg cgctcaacaa cgcgcagcat
    33361 catcggcaca tcaacctttt cattgagcaa ctgcgtcggt gcggcttcta ttttcgacag
    33421 cgtcagcaat tgcttcacca gcccttccat ccgctgggtc tgctcgcgca tggtgtgcaa
    33481 cgctttttcg cgtaccgcgc cttccagcgg ctgctcatcc atcatctcca ggtaaccctg
    33541 taacacggtc aatggcgtac gtaactcatg gctcacgttg gcaaaaaagt tgcgacgcgc
    33601 cccttccagt tgatgcattt gcgtgacatc acgcgccacc atcagcaact gtttgtgggt
    33661 ataaggcata acgcgaattt ccagatgccg cccggtgttg agcaccagat taagcgggcg
    33721 agaaaaatca cgcgttttca gatattgcgt aaactccggg taacgcagca ggttaaggat
    33781 gttctgcccg ttatcttccg gccagcgcaa accaagaatt tgttgcgcca gaccgttaca
    33841 ccagaaaata ccgccctctt ccgtggtcag caccaccgcg tcgggcagcg actccgcgcc
    33901 gctacgaaag cgtttaatca gattgcccag ttcacggcgg cgttttttat ttcgcagctg
    33961 catctggtgt aagccgtata gtagcggttc ccagctacca cgccccggtg gcggggtcat
    34021 actacgatcc acccacagcc accatgaaag gcgcaataaa ttccagaaat gccagataag
    34081 cagtcctgtt accgatgcca gcaaaaacca gggcaggtaa ccaaaaaatg cacccaggat
    34141 gaaagccggg aggcagcaaa gtagcagctc cagcaccagc cttttccacg acagccgttc
    34201 cagcacgtaa gatactccag ttaagaaatc ataagccctg ctctacgtcc gatgagcaag
    34261 gccttaaaag cgggttgaaa aacgataccc tgtaccgcgc acggtctgca ccatgcggtc
    34321 atgcccgccg ggctccagtg ctttacgcag gcgacgaatg tggacatcga ccgtacgatc
    34381 ttccacatac acgttagttc cccagacgtg gtttaacagc tgctcgcggc tgtacacgcg
    34441 ctcaggatgt gtcataaaaa agtgcagcag tttaaattct gtcggcccca tctccagtgg
    34501 ctcttcgccc gccatcaccc ggtgagatgt cgggtcgagg cttagcccct gcatctcgat
    34561 cacctcttcc accgccattg gcgaaatacg gcgcattacc gctttgattc gcgccaccag
    34621 ctccttcgga gaaaacggct tggtgatata gtcatccgca ccggtttcaa ggccgcgcac
    34681 gcgatcttct tcttcccctc tggcggtcaa catcaccact ggaatatccc gggtcatcga
    34741 ctcgcgcttg aggtgtttga tgaactggat accggagccg ccaggtaaca tccagtcaag
    34801 gagaattaaa tccggccagg gttcattcag ttgattcaca gcactgtcat aatcttccgc
    34861 ttcgaccggc tgaaagccat tttgttcgag cacgaagcag accatttcgc gaattggagc
    34921 ttcatcttct acgaccagaa tacgtctcgc catgatttgc cctgttgtaa taaataggtt
    34981 gcgatcatta atgcgacgtc attatgcgtc agatttatga cagatttatg aaaagctcgt
    35041 cgcacatatc ttcaggttat tgatttccgt ggcgcagaaa aaagcaaatg gcacatctgt
    35101 ttgggtataa tcgcgcccat gctttttcgc caggaaaccg ttatgcgcat ccttcacacc
    35161 tcagactggc atctcggcca gaacttctac agtaaaagcc gcgaagccga acatcaggct
    35221 tttcttgact ggctgctgga gacagcacaa gcccatcagg tggatgcgat tattgttgcc
    35281 ggtgatgttt tcgataccgg ctcgccgccc agttacgccc gcacgttata caaccgtttt
    35341 gttgtcaatt tacagcaaac tggctgtcat ctggtggtac tggcaggaaa ccatgactcg
    35401 gtcgccacgc tgaatgaatc gcgcgatatc atggcgttcc tcaatacaac cgtggtcgcc
    35461 agcgccggac atgcgccgca aatcttgcct cgtcgcgacg ggacaccagg cgcagtactg
    35521 tgccccattc cgtttttacg cccacgtgac attattacca gccaagcggg gcttaacggt
    35581 attgaaaaac agcagcattt gctggcggct attactgatt attatcaaca acactatgcc
    35641 gatgcctgca aactgcgcgg cgatcagcct ctgcccatca tcgccacggg acatttaacg
    35701 accgtgggtg ccagtaaaag tgatgccgtg cgtgacatct atattggcac gctggacgcg
    35761 tttccggcac aaaactttcc accagccgac tacatcgcgc tcgggcatat tcaccgcgca
    35821 cagattattg gcggcatgga acatgttcga tattgcggtt cccccattcc actgagtttt
    35881 gatgaatgcg gtaagagtaa atatgtccat cttgtgacat tttcaaacgg caaattagag
    35941 agcgtggaaa acctgaacgt accggtaacg caacccatgg cggtgctgaa aggcgatctg
    36001 gcgtcgatta ccgaacagct ggaacagtgg cgcgatgtat cacaggagcc acctgtctgg
    36061 ctggatatcg aaatcactac tgatgagtat ctgcatgata ttcagcgcaa aatccaggca
    36121 ttaaccgaat cattgcctgt cgaagtattg ctggtacgcc ggagtcgtga acagcgcgag
    36181 cgtgtgttag ccagccaaca gcgtgaaacc ctcagcgaac tcagcgtcga agaggtgttc
    36241 aatcgccgtc tggcactgga agaactggag gaatcgcagc agcaacgtct gcagcatctt
    36301 ttcgccacga cgttgcatag cctcgccgga gaacacgaag catgaaaatt ctcagcctgc
    36361 gcctgaaaaa cctgaactca ttaaaaggcg aatggaagat tgatttcacc cgcgagccgt
    36421 tcgccagcaa cgggctgttt gccattaccg gcccaaccgg tgcggggaaa accaccctgc
    36481 tggacgccat ttgtctggcg ctgtatcacg aaactccgcg cctttctaac gtttcacaat
    36541 cgcaaaatga tctcatgacc cgcgataccg ccgaatgtct ggcggaggtg gagtttgaag
    36601 tgaaaggtga agcgtatcgc gcgttctgga gccagaatcg ggcgcgtaac cagcccgacg
    36661 gtaatttgca ggtgccacgc gtagagctgg cgcgctgcgc cgatggcaaa attctcgccg
    36721 acaaagtgaa agataagctg gaactgacgg cgacgttaac cggactggat tacgggcgct
    36781 tcacccgttc gatgctgctt tcgcaggggc aatttgctgc cttcctgaat gccaaaccca
    36841 aagaacgcgc ggaattgctc gaggagttaa ccggcactga aatctacgga caaatctcgt
    36901 cgatggtgtt tgagcagcac aaatcggccc gcacagagct ggagaagctg caagcgcagg
    36961 ccagcggcgt cgcgttgctc acgccggaac aagtgcaatc gctgacagcg agtttgcagg
    37021 tacttactga cgaagaaaaa cagttaatta ccgcgcagca gcaagaacaa caatcactaa
    37081 actggttaac gcgtctggac gaattgcagc aagaagccag ccgccgtcag caggccttgc
    37141 aacaggcgtt agccgaagaa gaaaaagcgc aacctcaact ggcggcgctc agtctggcac
    37201 aaccggcacg aaatcttcgt ccgcactggg aacgcatcgc agaacacagc gcggcgctgg
    37261 cgcatactcg ccagcagatt gaagaagtaa atactcgctt acagagcaca atggcgcttc
    37321 gcgcgagcat tcgccaccac gcggcgaagc agtcagcaga attacagcag cagcaacaaa
    37381 gcctgaatac ctggttacag gaacacgacc gcttccgtca gtggaacaac gaactggcgg
    37441 gttggcgtgc gcagttctcc caacaaacca gcgatcgcga gcatctgcgg caatggcagc
    37501 aacagttaac ccatgctgag caaaaactta atacgcttgc ggcgatcacg ttgacgttaa
    37561 ccgccgatga agttgctagc gccctggcgc aacatgccga gcaacgccca ctgcgtcagc
    37621 gcctggtcgc gctgcatggg cagattgttc cccaacaaaa acgtctggcg cagttaatgg
    37681 tcactatcca gaatgtcact ctggaacaga cgcaacgtaa tgccgcactt aacgaaatgc
    37741 gccagcgtta taaagaaaag acgcagcaac ttgccgatgt gaaaaccatt tgcgagcagg
    37801 aagcgcgcat caaaacgctg gaagcccagc gcgcgcagtt acaggcgggt cagccttgtc
    37861 cactttgtgg ttccaccagc catccagcgg tcgaggcgta tcaggcgctg gaaccgggtg
    37921 ttaatcaggc ccggctatta acgctggaaa aagaagtgaa aaagctcggc gaagaaggtg
    37981 cggcgctgcg cgggcaactg gatgcattaa cgaagcagct tcagcgcgat gaaaacgaag
    38041 cgcaaagcct ccgacaagat gagcaagcac ttactcaaca atggcaagcc gtcacggcca
    38101 gcctcaatat caccctgcag ccacaggacg atattcaacc gtggctggat gcacaagatg
    38161 agcatgaacg ccagctgcgg ttactcagcc aacggcatga attacaaggg cagattgccg
    38221 cgcataatca gcaaattatc cagtatcaac agcaaattga acaacgccag cagcaactct
    38281 taacggcatt ggcgggttat gcgctgacat tgccacagga agatgaagaa gagagctggc
    38341 tggcgacgcg tcagcaagaa gcgcagagct ggcagcaacg ccagaacgaa ttaaccgcgc
    38401 tgcaaaaccg tatgcagcag ctgacgccga ttctggaaac gttgccgcaa agtgatgaac
    38461 tcccacacag cgaagaaact gtggcgctgg aaaactggcg gcaggttcat gaacaatgtc
    38521 tcgcattaca cagccagcag cagacgttac agcaacagga tgttctggcg gcgcaaagtc
    38581 tgcaaaaagc ccaggcgcag tttgacaccg cgctacaggc cagcgtcttt gacgatcagc
    38641 aggcgttcct tgcggcgcta atggatgaac aaacactaac gcaactggaa cagctcaagc
    38701 agaatctgga aaaccagcgc cgtcaggcgc aaacgctggt cactcagaca gctgaaacgc
    38761 tagcacagca tcagcaacac cgacctgacg ggttggctct cactgtgacg gtggagcaga
    38821 ttcagcaaga gttagcgcaa actcagcaaa agttgcgtga aaacaccacc agtcagggcg
    38881 agattcgcca gcagctgaag caggatgcag acaaccgtca gcaacaacaa accttaatgc
    38941 agcaaattgc tcaaatgacg cagcaagttg aggactgggg atatctgaat tcgctaatag
    39001 gttccaaaga gggcgataaa ttccgcaagt ttgcccaggg gctgacgctg gataatttag
    39061 tccatctcgc taatcagcaa cttacccggc tgcacgggcg ctatctgtta cagcgcaaag
    39121 ccagcgaggc gctggaagtc gaggttgttg atacctggca ggcagatgca gtacgtgaca
    39181 cccgtaccct ttctggcggc gaaagtttcc tcgtcagtct ggcgctggcg ctggcgcttt
    39241 cagatctggt cagccataaa acgcgtattg actcgctgtt cctcgatgaa ggttttggca
    39301 cgttggatag cgaaacgctg gataccgccc ttgatgcgct ggatgccctg aatgccagtg
    39361 gcaaaaccat cggtgtgatt agccacgttg aagcgatgaa agagcgtatt ccggtgcaga
    39421 tcaaagtgaa gaagatcaac ggcctgggct acagcaaact ggaaagtgcg tttgcagtga
    39481 aataactatt cagcaggata atgaatacag aggggcgaat tatctcttgg ccttgctggt
    39541 cgttatcctg caagctatca ctttattggc tacggtgatt ggtagccgtt ctggtggttg
    39601 tgatggtggt atgaaaaaag tcattttatc tttggctctg ggcacgtttg gtttggggat
    39661 ggccgaattt ggcattatgg gcgtgctcac ggagctggcg cataacgtag gaatttcgat
    39721 tcctgctgcc gggcatatga tctcgtatta tgcgctgggg gtggtggtcg gtgcgccaat
    39781 catcgcactc ttttccagcc gctactcact caaacatatc ttattgtttc tggtggcgtt
    39841 gtgcgtcatt ggcaacgcca tgttcacgct ctcttcgtct tacctgatgc tcgccattgg
    39901 tcggctggta tccggctttc cgcatggcgc attttttggc gtcggcgcga tcgtgttatc
    39961 aaaaattatc aaacccggaa aagtcaccgc cgccgtggcg gggatggttt ccgggatgac
    40021 agtcgccaat ttgctgggca ttccgctggg aacgtattta agtcaggaat ttagctggcg
    40081 ctacaccttt ttattgatcg ctgtttttaa tattgcggtg atggcatcgg tctatttttg
    40141 ggtgccggat attcgcgacg aagcgaaagg aaagctgcgc gaacaatttc actttttacg
    40201 cagcccggcc ccgtggttaa ttttcgccgc caccatgttt ggcaacgcag gtgtatttgc
    40261 ctggttcagc tacgtaaagc catacatgat gtttatttcc ggtttttcgg aaacggcgat
    40321 gacttttatt atgatgttag ttgggctagg gatggtgctg gggaatgtgc taagtggccg
    40381 gatttcagga cgttattcac cactgcgcat tgcggcagta actgacttta ttattgtgct
    40441 ggcactgctg atgctctttt tcttcggcgg catgaaaaca acgtcgctta tttttgcatt
    40501 tatttgttgc gcgggattat ttgccctttc agcaccactg caaatattgt tactgcaaaa
    40561 tgccaaaggc ggagagttat taggtgccgc aggtgggcaa atagcgttta acctcggtag
    40621 cgccgtcggc gcatattgcg gtggtatgat gctgacgctg gggctggcat ataattacgt
    40681 ggcgctgcct gccgccctgc tttcgtttgc tgcgatgtcg tcgttgctgc tgtatggtcg
    40741 ctataagcgc cagcaagcgg cggatagtcc ggtgctggcg aaaccactgg ggtagattac
    40801 tgtcaccgtg cgcgttgctt attgccggat gcggcgtaaa cgccttatcc ggcctacggg
    40861 ctgctcattt tgtaggcctg ataagacgcg gcaagcgtcg catcaggcat gatgcgctaa
    40921 ttgcctggtt tttttactct tgtggccata accacgcagc gccgcgtacg ccgctggaat
    40981 caccgtgctt cgccttacgc accggcgttt cacattcgcc gccgaagaca aattgtttaa
    41041 tcaactgcgg aaccgtttga tataaacggt ctacattgct catcccgccc cccagcacaa
    41101 tcacatccgg atcgagaata ttcacgacat gtgccagcga ttttgccagc cgcagctcgt
    41161 agcgacgcaa tgccagttcc gctaccggat cgctttcttc aaccaggcgg ataatttcac
    41221 tgcctttcag cgcatgtccg ctcaaacgac gataatccgt cgcaaatccc gtgcccgaaa
    41281 taaaggtttc aatacaacct tgtttaccgc aataacaagg gacttcctcg cgataacgca
    41341 gttcgtcttc gtccatccac ggtagcggat tgtgtcccca ctcacctgcc gtgccattgc
    41401 cgccgatatg cgcccgccca ttgaatgcca cgcccgcgcc gcatcccgtg ccgataatca
    41461 cggcaaatac cgtctgcgct cccgctgccg cgccatctac tgcttctgaa accgccagac
    41521 agttggcgtc atttgccagc cgcacttccc gctgcaacct cgcgcttaag tctttatcga
    41581 atggctgacc gttgagccag gttgaattgg cattcttcac cacgccggtg tagggcgaaa
    41641 tcgaaccagg aatgcccata cctaccgttc cgcgctgccc tgttgcctgc tccgccatat
    41701 caaccaacgt ggcgatcgtt tcaatagtct gccgataatc atcacgcggc gtgggcagac
    41761 gatggcggta caactgctcc cctgcatcgc ccagtgcaat cacttcagtt ttggtgccgc
    41821 ctaaatcgat acctatacgc acggtactct ccttattttt ttcaatatca atagcgtaga
    41881 gacggacaac tggattggca atgcaaggcc gccgacaatt cgttatcatg cccgctaaat
    41941 ttaacgacaa ggccgtggaa attatcatgc tgtggttcaa aaatttaatg gtttaccgtc
    42001 ttagccgcga gatttcgttg cgtgcagaag agatggaaaa acagctagcc tcgatggcat
    42061 ttaccccatg cggcagccag gacatggcga agatgggctg ggttcctccg atgggatcgc
    42121 acagcgatgc gttaacgcac gttgccaatg gtcaaattgt tatctgcgcg cgcaaagaag
    42181 aaaaaatcct cccgtctccg gtgattaaac aggcgctgga agcgaaaatc gccaaactgg
    42241 aagcggaaca ggcgcgtaag ctgaagaaaa ccgaaaaaga ttcgctgaaa gacgaagtgc
    42301 tgcactctct gctaccgcgt gctttcagcc gttttagcca gacaatgatg tggatcgaca
    42361 cggttaacgg tttgattatg gtggactgcg ccagtgccaa aaaagcggaa gatacgctgg
    42421 cactgctgcg taaaagcctg gggtcgttac cggttgtacc attgagcatg gaaaacccga
    42481 ttgaactgac gctgaccgaa tgggttcgct ccggcagcgc ggcacagggc ttccagctgc
    42541 ttgatgaagc cgagctgaaa tcgctgctgg aagatggtgg agtgatccgc gcgaagaaac
    42601 aagatctgac cagcgaagag atcaccaatc acatcgaagc cggaaaagtg gtgactaaac
    42661 tggcgctcga ctggcagcag cgcattcagt ttgtgatgtg cgacgatggt tcgctcaagc
    42721 gtctgaagtt ctgcgacgag ctgcgcgatc aaaacgaaga tatcgaccgt gaagatttcg
    42781 cccagcgttt tgatgccgat ttcatcctga tgactggtga actggcagcg ttaattcaaa
    42841 acctgattga aggattaggt ggcgaagcac aacgttaatt gctgatttac ctttaatgcc
    42901 ggatgcgacg cctgccgcgt cttatccggc gtacgaagct acaccaggca tataattatt
    42961 cgctacggcg ggcaataatt tttagcgcag caatattatg cgttttacgc tgtaacttgc
    43021 tccaggggcg ttgtgtcatt gtttttcctc aagccggtga cgcgcttcac tgcgaatttt
    43081 ccgcgttgcc ccggttttca ctaaatcccg taactgcgaa agagcgggtt gatcgacaaa
    43141 taaaagtaat gatggctcct cttttagcca caccgttttc acatcggcag ccagctgtgg
    43201 attattaata gccagcgacc gatcctgaga ctcagccaat gtcgttaaca acaccggggt
    43261 atgtgcgttg cgcaatgccg tttggcgcaa atgatcattg cgactttgca gcaatgcacg
    43321 ccaccattca tcatctgatt tatcttttat cgtcgccagt tccgcaacaa tatctggaga
    43381 tcttcgctga tatttatatc aaccttgatg aattatgaat atatcggaaa tcgaaaaaat
    43441 cccagcaaag gaaatattat tatgggttaa agagaggaca tttcagaaaa atgccctccc
    43501 gttcaagggg aaggcgagga attacagata gcggcacaga taagaggtgg gttcggcaac
    43561 ttgcagatga aactcgctgt gaccgggaac attaaacacc gaaccggctt catacacttg
    43621 ccagtcggtc gcgtcaggca gtaacacatt cagcgcgcca ctgattaccg tcatctcttc
    43681 aggctcagcg gtgctgaagg tgtattcacc ttcaaccata acacccacgc tggcgcgacc
    43741 agtgctgctg ctggaaaagc cgatcgattt cactttgcct gaaaagtact cattactttg
    43801 aagcataaac tggccctgtc tggtagttga acatctactt cactataggg gccagaggcg
    43861 ctggctgtca cgcaaaatta caccagtaat tccgcagcca gccgtgcaat caatacgtta
    43921 gagagcaaga cagtaacatc gagctgtttt tgcagtaaat cgcgatgacg ctggttaaat
    43981 cccaaacaat ccagcatgat gacatcagcc ccttttgcca gtaattcttt cccggcatca
    44041 atgatttttt gttccgaatc atgaatgggg ttacccaatg aaaatactgg cggtttctgc
    44101 aaaatttgcc atttttgcgc ctgaacggtc agcaactcct caaccggtac gataaccccc
    44161 acctgatgat cttcaacaat agaggaaacc agcggtggca atatccgcga cggctcaaga
    44221 aagatcgtat tacgcgcggt catactacta atgtttgctg tactcattaa tataatgacg
    44281 tcgtaaccct gattatcgag cacttcaacc acaccttgca ggtctcgttc cactttacga
    44341 cgcgaaacat gggccagctg gttgtcattt aataatgtga gaattgtgtc ttcgcctgct
    44401 tctggcgcgt actccgccat cacttcttcg cggcttaact tccccagcag gctatgatgg
    44461 gaaatattat cttcgtcaat gtattctgtc aggagcggca aaacttcctg cataggtaca
    44521 atgccgatgg tcaggatcgc caacgacgca ctcattgttc ttccacctta cttttatttc
    44581 gtcgttaatt accgggcaag cgctaagcac cactgctgac acttacctgt gctacaaatg
    44641 acaaaaagcg tagcagcaga cctgcacggc ataatgtaaa gattccaaat gattccagta
    44701 atggattgtt attgtttaat attctaatta tttgaaaaac atgaattgtg aaaaatgtga
    44761 cgcagatccc ttgtggtggc actcagaatc ccttccgagc agtctgatgc ggtcactctt
    44821 tgtcagggtc ctcatagcgt tttttcgcat ccatcgcttc ctgagcggtc ggatgttcac
    44881 tgatcaacga gtctggttta ggatgatcgg ctctgagttg gtaccaggtt accgtctgtc
    44941 ctggttttcc tttttcaatc gtcactatat atgcttcacg aggataaggc ggtttcgttg
    45001 gcatcgttct tccttatttc acgggatgaa cgttaagtat aggcgctcga aaatcaacaa
    45061 ttgatcgtct gtgccagggc actgcgaatt tcagaaatca cctggctggg ttcgtttgtt
    45121 gcgtcgatga taatatgcgc aacttcgcga taaagcgcat cgcgttcttc cagcacttcc
    45181 tgaacttctt cgctcagcgg tttccccgtt aaggttggcc gtaaatcttc ttccggagca
    45241 gcttgcagcc ggttaaccag gactgatact ggcgcacaca aataaaccac gatcccgtta
    45301 ttttgcatga agtgacgatt aaattccgtc agaataatgc cgccgcctgt agcgataacg
    45361 gtggatggcg cagttaccgc ttccagcgcc gccgtttctc tggcgcggaa tcccgcccac
    45421 tcttcccttt cgacgatctc cgcgaccgtc atattgagct gtgattgcaa ccactggtcg
    45481 gtatcgacaa accgacggtt aagcgaatcg gcaagggcca ttccgaccgt tgttttacca
    45541 cagccccgag gcccgatcag aaaaagaggt tgtgtcatcg tgggttttcc ccaataggtc
    45601 gcaatgcggc gaaagccggt atcatgagaa tagcgatcat accatcaaac tagtacaatt
    45661 tcgattgtaa agaaaaaatt ccacttaaag tgaaaatatc aatacatccc ttactatacc
    45721 aataaatatt caagaatgaa gtgtaaataa taaattacat ttagccacga ctacgttgca
    45781 cttccagcca ccacttatcc agctccgcag caaaggcctg gcggtcacgt tgtgaaagac
    45841 tatctggtcc tccggtctgg atcccactgg cacgtaaggt atccataaaa tcgcgcatcg
    45901 tcaggcgctc acgaatggtc gctggcgtgt aacgttcgcc gcgaggatta agcgccgccg
    45961 cgcctttctc gatggcttca gcagccagag gtatatctgc ggtgatcacc aaatcgcccg
    46021 cttcacactg ccggacaatt tcgttatcgg caacgtcgaa acctgccgcg acgcgcagcg
    46081 tacgaataaa tcgcgatggc ggcacgcgta aactctggtt tgctaccagt accagcggca
    46141 tctgcatacg ttccgccgcg cgatacaaaa tctctttaat tacattggga cacgcgtcgg
    46201 catccaccca aattgtcata aagtcatcct ttgttgggta atcctctatt gtgtcgcgct
    46261 tttgccttcc ggcatagttc tgtttatgct tctgccagcg attatcaaaa caatgaattt
    46321 cacggcagga gtgaggcaat ggaaaagaaa atcggtttta ttggctgcgg caatatggga
    46381 aaagccattc tcggcggtct gattgccagc ggtcaggtgc ttccagggca aatctgggta
    46441 tataccccct ccccggataa agtcgccgcc ctgcatgacc agttcggcat caacgccgca
    46501 gaatcggcgc aagaagtggc gcaaatcgcc gacatcattt ttgccgccgt taaaccaggc
    46561 atcatgatta aagtgcttag cgaaatcacc tccagcctga ataaagactc tctggtcgtt
    46621 tctattgcag caggcgtcac gctcgaccag cttgcccgcg cgctgggcca tgaccggaaa
    46681 attatccgcg ccatgccgaa cactcccgcg ctggttaatg ccgggatgac ctccgtaacg
    46741 ccaaacgcgc tggtaacccc agaagatacc gctgatgtgc tgaatatttt ccgctgcttt
    46801 ggcgaagcgg aagtaattgc cgagccgatg atccacccgg tggtcggtgt gagcggttct
    46861 tcgccagcct acgtctttat gtttatcgaa gcgatggccg acgccgccgt gctgggcggg
    46921 atgcctcgcg cccaggcgta taaatttgcc gctcaggcgg taatgggttc cgcaaaaatg
    46981 gtgctggaaa cgggagaaca tccgggggca ctgaaagata tggtctgctc accgggaggc
    47041 accaccattg aggcggtacg cgtactggaa gagaaaggct tccgtgccgc agtgatcgaa
    47101 gcgatgacga agtgtatgga aaaatcagaa aaactcagca aatcctgatg atttttgccg
    47161 gacgtcaggc cgccacttcg gtgcggttac gtccggcttt ctttgctttg tagagcgcca
    47221 aatcagccga tttcaaccac tcgcgatagt gactcatttg tggattcagc ggcgcaaccc
    47281 ccacactaat ccgtaatgtt acctgtggcg tattcggcaa acgtaacgtg tttagccctt
    47341 catgcacccg taacatggcg gtaatggcgc tctcagctgg tgtaccggac atgattacag
    47401 caaactcatc gccgccaaac cgaccaatca catcgctacc gcgcagggtt atttgtaact
    47461 gtcgggtaag cgccacaatc gcttcatcgc ccacatcatg gccccaggta tcgttgatgc
    47521 tcttgaaatg gtcgatatcg ataatcagta atgtcgcatc gcggttatgc cgccgacagt
    47581 tatcaaattc attgcgtaac atagtttccc aatgacgtcg gttatacacg cccgtcatac
    47641 cgtcgcgggt actcatgacc tgcaacctgc gtttatgttc cgccagttta gtcgccgtct
    47701 ggtagctgac ccagccaaac agcagaggat aaatgacgat aatgggaagg gagagccacc
    47761 attccagcgg cgcactattg aacgacacgg tgatgcccgt cagttcgagg gtgacaaggc
    47821 aggaaaccac catcaacacc agacccgcga caaacagacg ggggccgcct gcccccatca
    47881 aattcagaca cataatcatc aacatcgcgg tggaaggcag cacgtttacg cccattacgc
    47941 ctacccacat tcccgctaat actgcatcgg tttttaagtt gtaaatttcc cggctaagcg
    48001 gatcgacggc cctgctcgct atctgccagg ctaaatgcgg ccagacgaac gcccagccga
    48061 ccaacaccag ccaccaccag cccggcggcg ggtgcgaaac cagcgttgaa gcaatcggta
    48121 agaacatgcc agccaggcca accgcgcggg gtagtctgac gcgacgggcg aagcgcagcc
    48181 cggatcgctg atgttcgttt tgaggagtta aaggaggttc ctccccatgc gccgccgctt
    48241 ttttgaaaaa gttttcatca ttcattattt tgggaacatt cagaaacaat tttcccaaat
    48301 tatagagacg gggctaaagg cagaagtatg aaatttcggg tgagccattt tcgtagctca
    48361 cccgttagca ctatcaggta tcaggcggct ttcttgaggc aggcgctcat aaacttatta
    48421 cgatcatccc ctttcagaga ttgttgcgtt gcttgattat tacattcgcg catcttttgc
    48481 tgctgtggcg tcaaactttt ttcaccaggc gcagacttgc tgttcttcag gcaatcactc
    48541 atgtaggtct tacgagcatc ccctttcaac gcctgcgccg tcgcctgctg attacaggag
    48601 gtcatacgct gttgttgcgg ggttaaggtt ctctcggcag cgccgacggt ggttaaaaaa
    48661 accagtccga aaagcaaggt aaccagtaat gttattttca tcgcaccatc cctcttcatg
    48721 ttttaaccat gagcgtttgc gcccgtgatc tgccattaag tctggttgct aacagcaaaa
    48781 aaaccacccg acagcgaaaa ttcaccgccg ggcgcggttt tatttcagcc ccagggcggc
    48841 tttcatggtg tagaagagat cggtctggtc ggtcagtcca acgacattgg cggcatgtgg
    48901 gccatacgct gcaatacgca actgactgcc ggtatgttct tgtgaatcct cttcggagtt
    48961 cccgtaactc atcaccatca ctgcgccatc tttggtattt agcgcctggg tgaggcccgg
    49021 cgctttggtg tccggcgcga caatctggct ggcgtgggcg tgatcagcgg tgactatgac
    49081 cagcgtgttg ccatcctttt tagcgaattc cagcgcacgt tgtacggctt catcgagatc
    49141 gaccgtctcg ccaatttgcc cacaaggatt cgcagcgtga tcctgtttat cgattgatgc
    49201 accttcaact tgcaggaaaa agcctttctc atttttactc aacaattcaa tggctttgtc
    49261 ggtcatctgc gccagggtcg gtacactgtc attacgttgc ggattaggcg tacaggtaac
    49321 tgcgggcttg tcgatattgc cgtggtacgt tgctttcggt cctagccagc gcactggcat
    49381 attgccgtca gcaaacaggc caagcagggg tttttgctga ttcgcttccg tcaccgaatt
    49441 cagtgaggca gcatcactca ccaactgata accacgcgcc tgtgcctgtt cacgcagcgt
    49501 ttttccctgc cattcaccgg cggttgccgt ttcagcaaag gtttttgcac cgccgccaag
    49561 cgtaacatcg gcacgggcgt taagcagctg ttcggtaatc gatccttttc cgcctttttc
    49621 cagagcgtta cccggacatt tttcactggt cgcactcgga ccgtagcatt tgcgcgaggt
    49681 cacatgcgcc accagcgcag cgggcgtggc atcctgcaac tctgcggtag aaacgttacc
    49741 ggtcgccaga cctgcggctt ttgccatttc cagaatcgtt gggtgatctt tttcgtgaat
    49801 atcgacgccc agcgcgccgt tataggtttt gacaccagtt gaccaggcgg ttgctgatgc
    49861 agccgagtcg gtgacgtagt ccggtttgcc ggttttttta ttcagcgcat agtgtgtgta
    49921 ttgtccggta agcggtaagg catcgatacc tttaaaaaag ccgcccgcac cttcggcata
    49981 attgcgtgcg gcagtaattt ccgagtcccc catcccatcg ccaatcagca aaataatatt
    50041 ttttgcaggt ttatcgctaa gagaatcacg cagagcggcg gtctgatcac ccgttaagcg
    50101 gcgagcaccg ccgggcgcag taatatcgcc ctgagcagcc cggttttcca gaacaggcat
    50161 ttctggtgtc cgggcttttg tcacagggga aaacagtaac ggtaagagtg ccagtgcaat
    50221 agtgctttgt ttcactttat tttctccatg tacaaataca ttaaaaataa aaacaaagcg
    50281 actataagtc tcggccgtga caactttatg acagctgttg aaaagattaa ctttttactg
    50341 acgaggatgc ctcaataact tctttacgta atcgcgcagc agctccgtat cgtcgtcagg
    50401 aatgctggca tcgggcttta cctcgtacag cgccccctct acctgatcaa tcaaccgctg
    50461 ttgatcattt tgcgccatat tgcgaagcat tgccgtgacg ataatctcca aagcttctac
    50521 ctgcgcacac agttctttcg actcttcttc tttttgggca agcttaaata acaactcagc
    50581 aatgagattt ttcatgtctg tatttcctta tccaaagtat ggagaagtta acattatgtt
    50641 tcacaacttc atagtgtctg gaagtattat ttttatgaca aaggaaatac tttgcgcaaa
    50701 tattttttgt ttgattacca gcgaaacgtt tctct
//
LOCUS       DAPHCV010000019        50515 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.019, whole genome shotgun sequence.
ACCESSION   DAPHCV010000019 DAPHCV010000000
VERSION     DAPHCV010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.019"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(48..1289)
                     /gene="fabF"
                     /locus_tag="RH033_001112"
     CDS             complement(48..1289)
                     /gene="fabF"
                     /locus_tag="RH033_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDV2990335.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(1377..1613)
                     /gene="acpP"
                     /locus_tag="RH033_001113"
     CDS             complement(1377..1613)
                     /gene="acpP"
                     /locus_tag="RH033_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDV2990336.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(1824..2558)
                     /gene="fabG"
                     /locus_tag="RH033_001114"
     CDS             complement(1824..2558)
                     /gene="fabG"
                     /locus_tag="RH033_001114"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDV2990337.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ITDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(2571..3500)
                     /gene="fabD"
                     /locus_tag="RH033_001115"
     CDS             complement(2571..3500)
                     /gene="fabD"
                     /locus_tag="RH033_001115"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDV2990338.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAKASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(3516..4469)
                     /gene="fabH"
                     /locus_tag="RH033_001116"
     CDS             complement(3516..4469)
                     /gene="fabH"
                     /locus_tag="RH033_001116"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HDV2990339.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(4537..5607)
                     /gene="plsX"
                     /locus_tag="RH033_001117"
     CDS             complement(4537..5607)
                     /gene="plsX"
                     /locus_tag="RH033_001117"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDV2990340.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(5688..5861)
                     /gene="rpmF"
                     /locus_tag="RH033_001118"
     CDS             complement(5688..5861)
                     /gene="rpmF"
                     /locus_tag="RH033_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDV2990341.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(5913..6434)
                     /gene="yceD"
                     /locus_tag="RH033_001119"
     CDS             complement(5913..6434)
                     /gene="yceD"
                     /locus_tag="RH033_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HDV2990342.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            6633..7217
                     /gene="yceF"
                     /locus_tag="RH033_001120"
     CDS             6633..7217
                     /gene="yceF"
                     /locus_tag="RH033_001120"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HDV2990343.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(7329..8288)
                     /gene="rluC"
                     /locus_tag="RH033_001121"
     CDS             complement(7329..8288)
                     /gene="rluC"
                     /locus_tag="RH033_001121"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HDV2990344.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRIAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            8861..12046
                     /gene="rne"
                     /locus_tag="RH033_001122"
     CDS             8861..12046
                     /gene="rne"
                     /locus_tag="RH033_001122"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HDV2990345.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAATATPASPA
                     QPGLLSRFFGALKALFSGGEETKPAEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGNDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETVAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEVAEVVEAPVQVAEPQPEVAETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            12180..12473
                     /locus_tag="RH033_001123"
     CDS             12180..12473
                     /locus_tag="RH033_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HDV2990346.1"
                     /translation="MPAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEA
                     ARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPNAALRKTMQTPAPWEQKK"
     gene            12470..12964
                     /locus_tag="RH033_001124"
     CDS             12470..12964
                     /locus_tag="RH033_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV2990347.1"
                     /translation="MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTF
                     VSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEPIPVVVLGRLAIDKSLQGQGIDR
                     AMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
                     ECLK"
     gene            complement(13059..14012)
                     /gene="flgL"
                     /locus_tag="RH033_001125"
     CDS             complement(13059..14012)
                     /gene="flgL"
                     /locus_tag="RH033_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HDV2990348.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTESAPFSEADGKYEGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(14024..15667)
                     /gene="flgK"
                     /locus_tag="RH033_001126"
     CDS             complement(14024..15667)
                     /gene="flgK"
                     /locus_tag="RH033_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HDV2990349.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDASGKV
                     AFDDLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASAEDTGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(15733..16674)
                     /gene="flgJ"
                     /locus_tag="RH033_001127"
     CDS             complement(15733..16674)
                     /gene="flgJ"
                     /locus_tag="RH033_001127"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HDV2990350.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTSMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(16674..17771)
                     /gene="flgI"
                     /locus_tag="RH033_001128"
     CDS             complement(16674..17771)
                     /gene="flgI"
                     /locus_tag="RH033_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HDV2990351.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(17783..18481)
                     /gene="flgH"
                     /locus_tag="RH033_001129"
     CDS             complement(17783..18481)
                     /gene="flgH"
                     /locus_tag="RH033_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HDV2990352.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(18534..19316)
                     /gene="flgG"
                     /locus_tag="RH033_001130"
                     /pseudo
     CDS             complement(18534..19316)
                     /gene="flgG"
                     /locus_tag="RH033_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
     gene            complement(19487..20242)
                     /gene="flgF"
                     /locus_tag="RH033_001131"
     CDS             complement(19487..20242)
                     /gene="flgF"
                     /locus_tag="RH033_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HDV2990353.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQTTRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(20262..21467)
                     /gene="flgE"
                     /locus_tag="RH033_001132"
     CDS             complement(20262..21467)
                     /gene="flgE"
                     /locus_tag="RH033_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HDV2990354.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMTLVFNANGVLTSDPTANISTGAINGAEPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(21492..22187)
                     /gene="flgD"
                     /locus_tag="RH033_001133"
     CDS             complement(21492..22187)
                     /gene="flgD"
                     /locus_tag="RH033_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HDV2990355.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGTTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(22199..22603)
                     /gene="flgC"
                     /locus_tag="RH033_001134"
     CDS             complement(22199..22603)
                     /gene="flgC"
                     /locus_tag="RH033_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HDV2990356.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(22607..23023)
                     /gene="flgB"
                     /locus_tag="RH033_001135"
     CDS             complement(22607..23023)
                     /gene="flgB"
                     /locus_tag="RH033_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HDV2990357.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            23179..23838
                     /gene="flgA"
                     /locus_tag="RH033_001136"
     CDS             23179..23838
                     /gene="flgA"
                     /locus_tag="RH033_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HDV2990358.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLHTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            23914..24207
                     /gene="flgM"
                     /locus_tag="RH033_001137"
     CDS             23914..24207
                     /gene="flgM"
                     /locus_tag="RH033_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HDV2990359.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            24212..24628
                     /gene="flgN"
                     /locus_tag="RH033_001138"
     CDS             24212..24628
                     /gene="flgN"
                     /locus_tag="RH033_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HDV2990360.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNAANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(24668..26203)
                     /gene="murJ"
                     /locus_tag="RH033_001139"
     CDS             complement(24668..26203)
                     /gene="murJ"
                     /locus_tag="RH033_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDV2990361.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(26313..27236)
                     /locus_tag="RH033_001140"
     CDS             complement(26313..27236)
                     /locus_tag="RH033_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDV2990362.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVRAQRIVDKIWRDAMSE"
     gene            complement(27238..27885)
                     /locus_tag="RH033_001141"
     CDS             complement(27238..27885)
                     /locus_tag="RH033_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HDV2990363.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVMDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(27896..28480)
                     /gene="rimJ"
                     /locus_tag="RH033_001142"
     CDS             complement(27896..28480)
                     /gene="rimJ"
                     /locus_tag="RH033_001142"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HDV2990364.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            28716..29924
                     /gene="mdtH"
                     /locus_tag="RH033_001143"
     CDS             28716..29924
                     /gene="mdtH"
                     /locus_tag="RH033_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HDV2990365.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGTFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            29988..30635
                     /gene="grxB"
                     /locus_tag="RH033_001144"
     CDS             29988..30635
                     /gene="grxB"
                     /locus_tag="RH033_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HDV2990366.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            30769..31329
                     /locus_tag="RH033_001145"
     CDS             30769..31329
                     /locus_tag="RH033_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV2990367.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            31435..32481
                     /gene="pyrC"
                     /locus_tag="RH033_001146"
     CDS             31435..32481
                     /gene="pyrC"
                     /locus_tag="RH033_001146"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDV2990368.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVRKEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            32555..32800
                     /gene="dinI"
                     /locus_tag="RH033_001147"
     CDS             32555..32800
                     /gene="dinI"
                     /locus_tag="RH033_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HDV2990369.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            33090..33344
                     /gene="bssS"
                     /locus_tag="RH033_001148"
     CDS             33090..33344
                     /gene="bssS"
                     /locus_tag="RH033_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HDV2990370.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            33459..34577
                     /gene="solA"
                     /locus_tag="RH033_001149"
     CDS             33459..34577
                     /gene="solA"
                     /locus_tag="RH033_001149"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HDV2990371.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEEDPIFVRSGVINLGPADSTFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            34625..34738
                     /locus_tag="RH033_001150"
     CDS             34625..34738
                     /locus_tag="RH033_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HDV2990372.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVYYF"
     gene            34999..35565
                     /locus_tag="RH033_001151"
     CDS             34999..35565
                     /locus_tag="RH033_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HDV2990373.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHQAPELHKSIGILLMMGLVIRVLWRVISPPPGPLQSYSPMTRLGAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            35569..36144
                     /locus_tag="RH033_001152"
     CDS             35569..36144
                     /locus_tag="RH033_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HDV2990374.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(36186..37238)
                     /locus_tag="RH033_001153"
     CDS             complement(36186..37238)
                     /locus_tag="RH033_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDV2990375.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALDVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGINDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPSEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            37463..38383
                     /gene="lpxL"
                     /locus_tag="RH033_001154"
     CDS             37463..38383
                     /gene="lpxL"
                     /locus_tag="RH033_001154"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HDV2990376.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            38555..39781
                     /gene="mdtG"
                     /locus_tag="RH033_001155"
     CDS             38555..39781
                     /gene="mdtG"
                     /locus_tag="RH033_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HDV2990377.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            39864..40238
                     /locus_tag="RH033_001156"
     CDS             39864..40238
                     /locus_tag="RH033_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HDV2990378.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(40239..40466)
                     /locus_tag="RH033_001157"
     CDS             complement(40239..40466)
                     /locus_tag="RH033_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDV2990379.1"
                     /translation="MRLIVMSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(40638..43181)
                     /gene="mdoH"
                     /locus_tag="RH033_001158"
     CDS             complement(40638..43181)
                     /gene="mdoH"
                     /locus_tag="RH033_001158"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HDV2990380.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEVKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDVWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(43174..44709)
                     /gene="mdoG"
                     /locus_tag="RH033_001159"
     CDS             complement(43174..44709)
                     /gene="mdoG"
                     /locus_tag="RH033_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HDV2990381.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            45103..46260
                     /gene="mdoC"
                     /locus_tag="RH033_001160"
     CDS             45103..46260
                     /gene="mdoC"
                     /locus_tag="RH033_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HDV2990382.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(46268..47749)
                     /gene="clsC"
                     /locus_tag="RH033_001161"
     CDS             complement(46268..47749)
                     /gene="clsC"
                     /locus_tag="RH033_001161"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HDV2990383.1"
                     /translation="MMKKTPTSTKDSLLNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(47691..48224)
                     /gene="ymdB"
                     /locus_tag="RH033_001162"
     CDS             complement(47691..48224)
                     /gene="ymdB"
                     /locus_tag="RH033_001162"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HDV2990384.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(48319..48630)
                     /locus_tag="RH033_001163"
     CDS             complement(48319..48630)
                     /locus_tag="RH033_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV2990385.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAESTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(48751..49083)
                     /gene="csgC"
                     /locus_tag="RH033_001164"
     CDS             complement(48751..49083)
                     /gene="csgC"
                     /locus_tag="RH033_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HDV2990386.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(49142..49600)
                     /gene="csgA"
                     /locus_tag="RH033_001165"
     CDS             complement(49142..49600)
                     /gene="csgA"
                     /locus_tag="RH033_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HDV2990387.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGGNHGGGGNNSGPNS
                     ELNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSA
                     TLDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(49641..50096)
                     /gene="csgB"
                     /locus_tag="RH033_001166"
     CDS             complement(49641..50096)
                     /gene="csgB"
                     /locus_tag="RH033_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HDV2990388.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT        12250 a        13378 c        12427 g        12460 t
ORIGIN      
        1 aaaaaaggcc cgcaagcgga ccttttataa gggtgggaaa tgacaactta gatcttttta
       61 aagatcaaag aaccattagt gccaccgaag ccgaaggagt tacacagcgt gtattccatt
      121 ccgctgacct gacgtgcttc gtgcggtacg aaatccagat cgcaaccttc atccgggtta
      181 tccaggttga tggttggcgg aacagcctga tcgcgcagcg ccaggatgga gtagatagat
      241 tctactgcac ccgccgcacc taacaggtga ccggtcatag atttcgtgga gcttaccaac
      301 acacggcttg cagcttcacc gaagatggtt ttcaccgcct gcgcttcagc tttatcgcca
      361 gccggcgtag aagtaccgtg agcgttaacg tagccaatct gactcgcttc aatgcctgca
      421 tcacgcagag catttgccat tgccagagct gcgcctgcgc cattttctgg cggtgacgtc
      481 atatgataag catcgctgct cataccaaag ccgacgagtt cagcgtaaat tttcgcaccg
      541 cgttttttcg cgtgttcgta ctcttcaagt accagcatac cggcaccatc gcccagtacg
      601 aaaccatcac gctctttatc ccacgggcgg ctcgccgctt gcgggttatc attgcgggta
      661 gataatgcac gtgccgcgcc aaaaccacca acgcccagcg gcgtactggc tttctctgcg
      721 ccacctgcaa ccatcacgtc agcatcgcca tacgcgataa tacgcgcagc atggccaatg
      781 ttgtgcacgc cggaagtaca ggcggtcgcg atagagatgc ttgggccacg caggccatac
      841 atgatagtca gatgacctgc caccatgttc acaatcgttg acggaacgaa gaatgggctg
      901 atcttacgtg gaccaccgtt catcagagat gtgtggtttt cttcgatcag tccgaggccg
      961 ccaatcccgg agccaattgc ggcaccaatg cgggttgcgt tctcttccgt tatttcaagg
     1021 ccagaatcct gcatggcctg aacgccagcg acaattccat attgaatgaa ggcatccatc
     1081 ttgcgctgtt ctttgcgcga gataatgtcc tcacagttaa aatcctttac taagccagca
     1141 aatttcgttg cataggcgct agtatcgaaa tggtcgatta ggctgatgcc actctgaccg
     1201 gcaagcagag ctttccaggt agactctacg gtattgccga caggagacaa catgcccagt
     1261 ccggtcacaa ctacacgacg cttagacacg tttgtcctcc agggagggaa aaaatgattc
     1321 tagtgggaca aaaagataaa actcaggcgg tcgaacgacc gcctggagat gttcacttac
     1381 gcctggtggc cgttgatgta atcaatggca gcctgaacgg tggtgatttt ctcagcttct
     1441 tcgtccggaa tctcagtatc aaactcttct tccagagcca ttaccagctc aacggtgtca
     1501 agagaatccg cgcccaggtc ttcaacgaaa gaagcattgt tggtaacttc ttcctgctta
     1561 acgcccagct gttcgccgat aattttctta acgcgttctt cgatagtgct catactctta
     1621 aatttcctat caaaactcgc tttcgcgatg gttttcgtag tgtataaaat gttgaaaaat
     1681 ttgcaactaa atcccggcag gtcttaccac gattttacgt tattttgagg ccttaccccc
     1741 caataacgca aatcattttg cactaattat tactcataac cacgcagact gcgcgcaaca
     1801 tgagcatttt gtgcaaatcg cggtcagacc atgtacatcc cgccgttcac atgcaaagtt
     1861 tcacccgtga tgtaagctgc ttcgtcggat gccaggaatg caaccgcgtt ggcgatttcc
     1921 tgtgcgccgc cgaggcgacc cgcaggaacc tgcgccagga tacccgcacg ctggtcatcg
     1981 ctcagcgcac gtgtcatgtc cgtttcaata aagcccggag caacaacgtt tacagtaata
     2041 ccgcgtgacg caacttcgcg cgccagtgat ttactgaagc cgatcaagcc cgctttcgcc
     2101 gcagcgtagt tggcctgacc gccatttccc atggtaccaa ccacagaacc gatagtgata
     2161 atacgaccat gacgcttttt catcatagcg cgcattaccg cttttgacag acggaaaaca
     2221 gatgaaaggt tggtttcgat aatatcgttc cactcttcat ctttcattcg cattaacagg
     2281 ttatcacgag tgataccggc attattgacc aggatatcca cttcaccaaa ttctgcgcga
     2341 attttttcca gaacagattc gatagatgcc gggtcggtca cattcaacat cagacctttg
     2401 ccgttggcac ctaaataatc agtgatcgcc tgagcgccat tttcactggt cgcagtgccg
     2461 ataactttcg cgccacgggc tgcgagcgtt tcagcaattg cgcggccaat tccgcggctt
     2521 gcaccggtta ccagtgcgat ttttccttca aaattcatga ttttcctctt ttaaagctcg
     2581 agcgccgctg ccatcgctga aggttcgttc agcgccgagg cggtcagggt gtcgacaatg
     2641 cgtttcgtca ggccagtaag cactttgccc gggccgactt catagagatg ttctacgcct
     2701 tgcgctgcca tgtattcaac agacttcgtc cactgaaccg ggttatacaa ctgacgtacc
     2761 agtgcgtcac ggatggcatc accattggtt tcgcatttca catcaacgtt attcacaaca
     2821 ggaactgttg gtgcgttaaa ggtgattttc gctaattcta ctgccagttt gtcggctgct
     2881 ggtttcatca gcgcacagtg agacggtacg ctcactggta acggcagagc gcgttttgcg
     2941 cccgcagctt tacaggcagc gccagcacgc tcaactgctt ctttatgacc ggcaataacc
     3001 acctgtcccg gagagttaaa gtttaccgga gaaacgacct gaccttctgc agcttcttca
     3061 cacgctttcg caatagacgc atcatccaga ccgatgattg ccgccatagc acccgttcct
     3121 tccggtacgg cttcttgcat gaacttgccg cgcatctcaa ccagacgcac cgcatcagcg
     3181 aaatcaatca caccagcgca aaccagcgcg gagtattccc ccaggctgtg accggccatc
     3241 attgccggtg ctttaccgcc ctgctgctgc catacgcggt acagcgcaac agatgcagtc
     3301 aacagcgcag gctgggtttg ccaggtttta ttcagttctt cagctggccc ctgctgtgtc
     3361 agcgcccaca ggtcgtagcc cagcgccgca gaagctttag caaacgtttc ttcgacaatt
     3421 ggatagctcg ccgccatatc agccagcatt ccaacggttt gagaaccctg tccagggaac
     3481 acaaatgcaa attgcgtcat gttttaatcc ttatcctaga aacgaaccag cgcggagccc
     3541 caggtgaatc caccgccaaa ggcttcaagc agaaccaact gccccggctt aatgcgcccg
     3601 tcgcgtacag cttcatccag agcgcacggg acagaggccg cagaggtatt accgtggcga
     3661 tccagcgtca ccacgacatt gtccatcgac atgccgagtt ttttcgctgt tgcactgata
     3721 atacgcaggt tagcctgatg cggaaccagc cagtccagtt gagaacggtc aagattattc
     3781 gccgccagcg tctcatctac gatgtgcgcc agttctgtta ccgcaacctt gaagacttcg
     3841 ttgcccgcca tcgtcagatg aattgaattc tctggattca cacggtcagc attaggcagc
     3901 gtcagcaact caccatagct accgtcggca tgcagatggg tggagatgat tcccggctct
     3961 tcagaggcag ccagcaccgc agcgcccgcg ccatcgccaa aaataataat agtcccacga
     4021 tcggttggat cgcaggtgcg cgccagtaca tcggaaccga cgaccagagc atacttcacc
     4081 gccccagatt tcacgtattg atcggctacg cttaatgcat aggtgaaacc tgcgcaggct
     4141 gctgcaacgt caaatgccgg gcaaccttta atgcccagca tgctttgaat ctgacaagct
     4201 gcgctcggga aagcgtgcgt agctgaagtc gttgccacaa cgatcaggcc aatctggtct
     4261 ttctcaatgc ccgccatctc aattgcgcgt gtcgccgctt caaagcccat ggttgaaacg
     4321 gtttcgtttg gcgcggcaat gtggcgttcg cggataccgg tacgagtgac aatccactcg
     4381 tcagaggtgt ccaccatttt ttccaaatcg gcgtttgtcc gcacttgttc gggcagatag
     4441 ctgccagtac caataatctt cgtatacatg tacgctcagt cacttttcgg ttatataccg
     4501 tcacttgcaa actgcgagtt cgctggcagc gtcctgctac cgcagagttc cgcttttgcc
     4561 accgtccagc agctcaaaac cagctgggta tacagattcc aggcgagcgg caattcgctg
     4621 aggaacttgt cgctgcaccg cctgcactgc ctgttcaatc gcgaccgcaa aagctcgctg
     4681 attggctgca ccatgacttt ttatcaccgt gccgcgcaat cctaacagac aggcgccgtt
     4741 atactggtcg gggttgaggt gactgaatcg cctcgtcagg ctcttttgta gccaacgctt
     4801 taataacagt agccaccacg accgtttttt cccttcaccc tgagatttca gcagagaaag
     4861 gaacatcctg acaacacctt ccatcgtctt taatgtgaca tttcctgtaa agccgtcaca
     4921 aaccagcaca tctgtcttgc cagttaacaa ctcattggct tcaagatagc cgatataatt
     4981 gatagaaggg attgttttaa gcaccgctga ggcatcccga atactgtcga gaccctttac
     5041 ttcttcttca ccaatattga gcaacgccac gcgaggattg ggaatttcca ccacctcttc
     5101 agccaggact gagcccataa tggcaaattg caccaacatg gtgctgtcac aatcgacgtt
     5161 ggcccctaaa tcgaggacca ccgttttgcc cttttgctga tgcggtaata ccgtcaccag
     5221 cgccggacgc tcaatcccct ccaggggctt gagtaataat tttgccagcc ccatcagcgc
     5281 cccggtatta ccggcactga cacaggcttg cgctcgacct tctttcacca gctccagggc
     5341 cacgcgcatt gaactcccac gactggcgcg gatagcttgc gaaggccggg catcactggc
     5401 gataactgac tgcgcaggaa taatctgcag acgcgaacgt tgttcaaagt cagctttagc
     5461 aagtaatggc gtgatggcgt cgggattgcc gactaaaaga agagtgagtt gcgaattaga
     5521 attcagtgcc tgcaatgctg caggcactgt cacggaaggg ccaaaatccc ctcccatgac
     5581 atctaacgcc agggttagac gtgtcaaggt atcgtcgccg cccggtttgg tctttcccca
     5641 gttgcctggg gaaaatcctc actaagcttc atcacgcaga cgcgtgatta cttagcgatg
     5701 accttgcggc cgcggtagta accgtcggca gtgatgtggt gacgcaggtg tttttcacca
     5761 gaagttttgt ctacagacag gctggtgact gcggtcagcg cgtcatggga acgacgcatg
     5821 ccacgtttgg aacgggttgg tttattctgt tgtacggcca tggaccttac tcctcaatta
     5881 cggtttatcc ccgatccaac ggggagcacc aattacttac gctttaagct ggctaatacg
     5941 gcaaatgggt ttggcttttg cgcttcttca ggcagttcac caaagaccat gtccgcttcg
     6001 gacacttcac agtgttcaga atcatgcacc ggaactaccg gcaaggcgag gatgatttca
     6061 tcttcaacca ttgcaagcag atcgatttca ccgaattcgt taacctcaat cggttcatac
     6121 gcttccggca gtgcttcagc ctgttcgtct gaacgcacag gactaaaaca atacgttgtg
     6181 tagacctgat gagtaaacgg cttcccgcaa cgctgacact cgagcgttac cgtcaccttc
     6241 gcatcgccgt ttaacactgc gagacgttgg ttatcgatag cgaacgacat ggagcattcc
     6301 acatcactgt ccacactgac tacggattcg gcgacgcgct caacctgatc aggggtatag
     6361 ataccctggt aatcaaggcg tttttgagcc gtacgaaccg gatcgagagt caggggtaat
     6421 tttacctttt gcatagggcg cgcatattaa ctttgtaacg tcatagagtc aaagaaaaag
     6481 gcagcctgtg gttgcctttt gccaataatt cgcacacatt gcgggttact gctttatttt
     6541 cgttcagtgc tgataacgaa catcaacctg caaaacgaag cgcatagttt aaaatggcta
     6601 tcatcaatac gctatatctg atggaaaaaa atatgcctaa acttatttta gcctccacct
     6661 cgccctggcg tcgcgctctt ctggaaaaac tgcaaatctc ttttgaatgt gcagcacccg
     6721 aggtcgacga aactccacgc agcgacgaat caccgcgaca gttggtgctt cgactggcac
     6781 aagaaaaggc gcaatctctg gcgtcacgtt atccggatca tttaattatt ggttcagacc
     6841 aggtatgtgt tcttgatggc gaaatcaccg gtaaaccgtt aacggaagaa aatgcccgtc
     6901 tgcaattacg caaagccagc ggcaatatcg tcaccttcta tactggactg gcgttgttta
     6961 attcggcgaa tgggcatcta caaacagaag tggagccttt tgacgtccat ttccgtcacc
     7021 tgagcgaggc ggagattgat aattacgtgc gtaaagagca tcccctgcac tgcgcgggta
     7081 gctttaagag tgaaggattt ggcattactc tgtttgagcg tttagagggg cgtgatccta
     7141 acacgctggt tggtttaccg ctaatcgcgc tgtgtcagat gttacgtcgg gaagggaaaa
     7201 acccgctgat gggatagttt tttgcgccgg atgcggttac ttcgcatccg gcaatcggtg
     7261 ctggcggcct gatgcggcgt aaacgcctta tcaggccaac cgcccattat catgcttata
     7321 tgctcgtatt agcgcgcgtt acgcagtttt tgcaaacaac gcttcaaacc ttcatccatc
     7381 ggcgcttcga tacgcatcac ctcaccggtc cccggatgag taaacttcaa cgctgcagcg
     7441 tgcaggaaca ggcgatttaa tcccgtgcct gcttcagtga gctgtctgtc aaattcacgg
     7501 tcaccgtagc gatcgtcaaa ggcaatcgga tgacccgcat attgtgtatg cacacggatc
     7561 tgatgagtac gccctgttac cgggctacaa cgcaccaggg tggcaaatgc atagcgttct
     7621 tccactttaa agcgtgtttc cgacggtttg ccttcctgac tcacacgcac gatacgttcg
     7681 ccgctttgca gaatattttt cagtaacggt gcttgaacgc tcttcacatg cgactgccac
     7741 tgaccgcgca ccagcgccag gtaatctttt tgcatccctt tttcacgtag ttgctcatgc
     7801 agagaacgca acgccgagcg ttttttcgct accagcaaca cacctgaagt gtcccggtcg
     7861 agacgatgaa ccagttcaag gaaccgcgct tccgggcgca acgcccgcaa accttcgata
     7921 acgccgaagc ttaaaccact gccgccatgt accgccgtac cggaaggttt attcagcacc
     7981 aggatatggt catcttcata taagatgacg tccgccagcg ccgccacctt ttgcagatgt
     8041 ggcgaaaccg cctcttcttc ccgctcagca atgcgaaccg gtggaatacg cacctcatca
     8101 cccgcttcga gtttatattc aggcttaata cgttttttgt tcacccgcac ttcgccttta
     8161 cgcaaaatac ggtaaatcat actttttggt acgcctttca attgagtacg caaaaagtta
     8221 tcgatacgtt gccccgcttc gtcagcggtg atagcaacaa tttttacgga tggagtctct
     8281 gttttcatgg tgggcgattc taattagcca acaggattcg cgccactcat ttttctatgc
     8341 ttatatttag ttttgcacct tattacttca ctacgtgatc actttattga tggttattaa
     8401 accaatcacc agcaagaagt gaaaaaactg tgagtaagcg ggtgataaat ggtaaaagtc
     8461 atcttgctat aacaaggctt gcagtggaat aatgaggccg tttccgtgtc catccttgtt
     8521 aaaacaagaa attttacgga ataacccatt ttgcccgacc gatcatccac gcagcaatgg
     8581 cgtaagacgt attgatcttt caggcagtta gcgggctgcg ggttgcagtc cttaccggta
     8641 gatggaaata tttctggaga gtaataccca gtctgtttct ttgataattg cgctgttttt
     8701 ccgcatgaaa aacgggcaac cgacactctg cgcctctttg agctgacgat aaccgtgagg
     8761 ttggcgacgc gactagtcac gaggccatcg gttcacaccc ggaaaggcgt tactttgccc
     8821 gcagcttagt cgtcaatgta agaataatga gtaagttacg atgaaaagaa tgttaatcaa
     8881 cgcaactcag caggaagagt tgcgcgttgc ccttgtagat gggcagcgtc tgtatgacct
     8941 ggatatcgaa agtccagggc acgagcagaa aaaggcaaac atctacaaag gtaaaatcac
     9001 ccgcattgaa ccgagtctgg aagctgcttt tgttgattac ggcgctgaac gtcacggttt
     9061 cctcccacta aaagaaattg cccgcgaata tttccctgct aactacagtg ctcatggtcg
     9121 tcccaacatt aaagatgtgt tgcgtgaagg tcaggaagtc attgttcaga tcgataaaga
     9181 agagcgcggc aacaaaggcg cggcattaac cacctttatc agtctggcgg gtagctatct
     9241 ggttctgatg ccgaacaacc cgcgcgcggg tggcatttct cgtcgtatcg aaggcgacga
     9301 ccgtaccgaa ttaaaagaag cactggcaag ccttgaactg ccggaaggca tggggcttat
     9361 cgtgcgcacc gctggcgtcg gcaaatctgc tgaggcgctg caatgggatt taagcttccg
     9421 tctgaaacac tgggaagcca tcaaaaaagc cgctgaaagc cgcccggctc cgttcctgat
     9481 tcatcaggag agcaacgtca tcgttcgcgc attccgcgat tacttacgtc aggatatcgg
     9541 cgaaatcctt atcgataacc cgaaagtgct cgaactggca cgccagcata tcgctgcatt
     9601 aggtcgcccg gatttcagca gcaaaatcaa gctgtacacc ggcgagatcc cgctgttcag
     9661 ccactaccag attgagtcgc agattgagtc cgccttccag cgtgaagttc gtctgccatc
     9721 cggtggttcc attgttatcg acagcaccga agcgttaacc gccatcgaca tcaactccgc
     9781 acgcgcgacc cgcggcggcg atatcgaaga aaccgcgttt aacaccaacc tcgaagctgc
     9841 cgatgagatt gctcgtcagc tgcgcctgcg tgacctcggc ggcctgattg ttatcgactt
     9901 catcgacatg acgccagtac gccaccagcg tgcggtagaa aaccgtctgc gtgaagcggt
     9961 gcgtcaggac cgggcgcgta ttcaaatcag ccatatttct cgctttggcc tgctggaaat
    10021 gtcccgtcag cgtctgagcc catcactggg tgaatccagc catcacgtct gcccgcgctg
    10081 ctccggtacc ggtaccgtgc gtgacaacga atcgttgtcg ctctctattc tgcgtctgat
    10141 cgaagaagaa gcgctgaaag agaacactca ggaagttcac gccattgttc ctgtgccaat
    10201 cgcgtcttac ctgctgaatg aaaaacgttc tgcagtgaat gcgattgaaa ctcgtcagga
    10261 cggcgtgcgc tgtgtgattg tgccaaacga tcagatggaa accccgcact accatgtgct
    10321 gcgcgtgcgt aaaggggaag aaactccaac cttaagctac atgctgccga agctgcatga
    10381 agaagcgatg gcgctgccgt ctgaagaaga gttcgctgaa cgtaagcgtc ctgaacaacc
    10441 tgcgctggca acctttgcca tgccggatgt gccgccagct ccaaccccag ctgaacctgc
    10501 cgcgcctgtt gtagccccag cacctaaagc tgcaacggca acaccagcat ctcctgcaca
    10561 accagggttg ttgagccgct tcttcggcgc actgaaagcg ctgttcagcg gtggtgaaga
    10621 aaccaaaccg gccgagcaac cagcaccgaa agcagaagcg aaaccggaac gtcaacagga
    10681 tcgtcgcaag cctcgtcaga acaaccgccg tgaccgtaat gagcgccgcg acacccgtag
    10741 tgaacgtact gaaggcaacg ataatcgcga agaaaaccgt cgtaatcgtc gccaggcaca
    10801 gcagcagact gccgagacgc gtgagagccg tcagcaggct gaggtaacgg aaaaagcacg
    10861 taccaccgac gagcagcaag cgccgcgtcg tgaacgtagc cgccgccgta acgatgataa
    10921 acgtcaggcg caacaagaag cgaaggcgct gaatgttgaa gagcaatctg ttcaggaaac
    10981 cgaacaggag gaacgtgtac gtccggttca gccgcgtcgt aaacagcgtc agctcaatca
    11041 gaaagtgcgt tacgagcaaa gcgtagccga agaagcggta gtcgcaccgg tggttgaaga
    11101 aactgtcgct gccgaaccaa ttgttcagga agcgccagct ccacgcacag aactggtgaa
    11161 agtcccgctg ccagtcgtag cgcaaactgc accagaacag caagaagaga acaatgctga
    11221 taaccgtgac aacggtggca tgccgcgtcg ttctcgccgc tcgcctcgtc acctgcgcgt
    11281 gagtggtcag cgtcgtcgtc gctatcgtga cgagcgttat ccaacccaat cgccaatgcc
    11341 gttgaccgta gcgtgcgcgt ctccggaact ggcctctggc aaagtctgga tccgctatcc
    11401 aattgtacgt ccgcaagatg tacaggtcga agagcagcgc gaacaggaag aagtacaagt
    11461 gcagccgatg gtgactgagg tccctgtcgc cgccgctgtc gaaccggttg ttagcgcgcc
    11521 agttgttgaa gaagtggctg aagttgtaga agcccccgtt caggttgccg aaccgcaacc
    11581 ggaagtggct gaaacgacgc atcctgaagt aattgccgcc gcggtaactg aacagccgca
    11641 ggtgattacc gagtctgatg ttgccgtagc ccaggaagtt gcagaacacg cagaaccggt
    11701 ggttgaaccg caggaagaga cggctgatat tgaagaagtt gccgaaactg ctgaggttgt
    11761 ggttgctgaa cctgaagttg ttgctcaacc tgccgcgcca gtcgtcgctg aagtcgcagc
    11821 agaagttgaa acggtagccg cggttgaacc agagatcacc gttgagcata accacgctac
    11881 cgcgccaatg acgcgcgctc cggcaccgga atatgttccg gaggcaccgc gtcacagtga
    11941 ctggcagcgc cctacttttg ccttcgaagg caaaggtgcc gcaggtggtc atacggcaac
    12001 gcatcatgcc tctgccgctc ctgcgcgtcc gcaacctgtt gagtaataat tagctcaagt
    12061 aatcaagccc tggtaactgc cagggctttt ttatttcagg ggtagcatct ttgctgtaat
    12121 gacattagcg ttacatgtac tgtataatta tcatccacgc aaagccgaag gatatattta
    12181 tgccagcagc caataacatt ccagccaggc gcgaatcttt aaacttgcgc ataaaacccg
    12241 cagaacgcga tcttattgac cgggccgcta aagcccgagg aaaaaatcgc accgaatttg
    12301 tccttgaggc cgccagagcc gcagctgaag aggcgctcat tgagcagaga attttcagga
    12361 tcgattctga tgattatcag ctatttttga atcgtctgga tcagcccccc gcgcccaatg
    12421 ctgcattacg caaaaccatg caaacacccg cgccgtggga gcaaaaaaaa tgattcaggc
    12481 ccccgagcca ctcatggcaa ggcatcaatt cacctctttt tgctcgggag tagaaacgat
    12541 ggataactgg ctaaagcaga gagccctaaa aaatcagctt gccggcgcat cacgtacatt
    12601 tgttagctgt gatacgtata gcaacgtttt ggcatattat tcgcttgctt ccagcgccgt
    12661 tgaaacgtat gtcgcgacag gacgatttcg ccgaaatatg ccagaaccga tccctgttgt
    12721 agtacttggt cgtctggcaa tagataaatc attacagggt cagggcatcg atcgtgcgat
    12781 ggtcagagat gccggtctac gagtactaca ggcggctgaa gtcattggta ttcgcggaat
    12841 gttggtccat gcgttatcag accaggcccg tgagttttat ctgcgagtgg ggtttgaacc
    12901 atcgcctgtc gattctatga tactgatggc aacattggct gatttgcagg aatgtcttaa
    12961 atagttcgtt atgtctcatg caaaaaaatg cccgccccgg cgatcccaga gcaggcagac
    13021 aaaacatacc cagtttcatg atatgtttta aagcgaaatt atttgttgag ctggaagagc
    13081 gacaatccct gcatatcggt aaatgcttta tacgacgcct gcaatgccgt ttgctgcatg
    13141 atgtaagatg aaatagttgc attccagtcc acatcaacca gatcgctcat ctgctgcgtt
    13201 tgccctaaag cgcgatcgct acctaatgaa tccagcgact ccagttcgtt cagctgcgtg
    13261 cctaattccg cgcgaacagt cagcacattg ttcagcgagt ttttcagtcc gcggttggtt
    13321 ttatccagtg ccgcagcggc ggtttcttta tccgcttcgc tatccgcgac cggcgttttc
    13381 agggctgcga tggcgctatc cagcatggca aaaagattgg tttcagaagc gctaccgtct
    13441 ggttccgcta ccgcgttgct ggtaatactg tcgaaaattt tgtcacccgt gtgccctatc
    13501 accatcgaac gcgaagcatc gacctgttgt ttaatacttt ctgctccgcc ttcgtatttt
    13561 ccatccgcct cgctaaacgg tgcggattct gttttataac cggcaaaaat gtagcgcccg
    13621 ttaccgtcag tggtgttcgc cagattcagc aactggtcac gaagcccctg aatatccgta
    13681 gccagcgagg cccggtcatc gtcactcaag gtgccattgc tggcgtagac aattttttcc
    13741 tgagcattct ggatagcggt ggtgacctgg ctaagtacac tctcttccag tgacactttt
    13801 tgagtggcga aagtacgcgc cagcgtgtac tggctgtttt gcgcctgtgc ctgggagaga
    13861 acaacagctt gtgatgcagc aatgggatca tctgaagggt taacgactcg cttacccgtc
    13921 gacatctgtt cgccgtactt catccattct gcctgagaat tggtgatacc acgcatgttt
    13981 tgctggtaca tcatctgggt actgaaacgc atcttatctc cccttagcga atgttaatca
    14041 gcgcatcaaa aatcgcgttt gccgtctgca gaacctgcgc attcgccagg taatactgct
    14101 gaaaacgttg cagatttccg tactcctcat cgagattgac accggaaatc gactgctgct
    14161 gattggaaag ctgcgtcacc acattacctt gcgtggtgct actggttttc aacgtcgcgg
    14221 ttttattacc gatatcactc actaacgagg cataagcgtc gttaaaggat ttcgcaccgc
    14281 ccaccgtttt actgttgctt tgcagatcga gcagggcctg accgttgcgg ttgtcgctat
    14341 cacctgtatc ttctgcgctc gccatggcga ttttcgcttc gtcggtgatt aatacatcca
    14401 tgttgacgat ggcgtcactt accggtttca gcgtgaagct gtcgttaacg gcaggcgttc
    14461 ctgtaaacgt caactccaga tcatcaaatg ccactttacc gctggcatcc ggcgtcaccg
    14521 taaaagtggt attactggca aggcgggtga cctgccactg attattatcg aacgagattt
    14581 tgtaatctgt cgccagtacc gcggaggcat cagttaccgt tgcaccgatc gcaacgtcac
    14641 ctttgttttt cgtgttttgc agaaccgcgg gcttaccgat agcaaagaaa tcttcaccgg
    14701 catcgccgtt agcatcaaat ccggctttgt gttgggtgtt gaaagcctcg gcaaatgcca
    14761 gcgccagttg tccaagcgta ttacgcgtct ggtccagatc ctgagaacgg aatgtcagaa
    14821 tgccgcccag cgacccggta ttcagtaatt tctccgggat ctcaatattg cctgccgtcc
    14881 catcaacata agcgacagtc gtacgagaag ggtcagcgct ggaaggaact gccgccagtt
    14941 gccgagccgt acttccctga accagtgagt aaccattggc catcgtgatg ttataagtgc
    15001 caccatcctg aacgctgact tctacaccaa caatctggtt taattcgctc accagttgat
    15061 cgcgttgatc cagcagattg ttaggtgacg cccctgcccc cacgcctgtc aggcgggaga
    15121 tttgatcgtt caggctggcg atttgtttag cgtaattgtt gatctgatca acgctggcac
    15181 ctatcgcgat attgacctgt ttgtcctggt cgcgcagata ttgatcggtg gttttaaact
    15241 gattcaccaa cccttctgat ttcccaatca gcgcctggcg cgctgccgga tcttccgcgt
    15301 tactcaccag cgtttgcagg ctggtgaaga aatcctgcat ctgtgttgcc agcgaagagg
    15361 tactggtgga gagcatattg tcgattttcg acatctgctc atagcgggcg gtcaggccgc
    15421 tactttgcgt ctgcgcagca cgtaactggt tggtaataaa cgcatcatac tcacgctgca
    15481 caccagaaac gtagacgcca ttgccaaccc agccgccagc gcccaacgtg ctattggcct
    15541 gcgccataat agtggtttgg cgggtatatc cggcaacgtt atagctggag atattattac
    15601 ttgccgtatt taacgccgcc tgggccgcgt tcagtccgct catggcgtta ttgatcaagc
    15661 tggacatgga ggttccttat aagccttcaa ttacagagta ttatcggcag cgccccgccg
    15721 gacttgagtt attcagaaca gattatcaat gttcatgctg taggttttgc tcaccttgtc
    15781 gcttatcgat ttcatctgct gaatcatgct ggtgagtttg cgggcatagt gaggatcggt
    15841 ggcatagccc gcgtcctgta gggcctgcgc cccctgctcc gcactcgcgg cggtcgtcac
    15901 ggcggcgtag cgcgggttac gcgttaacag cccaacgtaa tccgacaatg cttccagata
    15961 cgagctgtag acccgaaact tcgcttttac tttcttcgct tcgccatttt catattcagt
    16021 cgtggtgatt tcagtgactg gccctttcca gttgccagag gctttgacac caaacaggtt
    16081 atagctcggc tcgccgtttt cacggcggat ttgccgttgt ccccagccag attccagcgc
    16141 cgcctgagcg aggatcaaat gatgtggcac accactttgc tggcttgcca gttgggctgg
    16201 cagcgagagt tgcgcgagga atgctttact gtcacccggc agcgaatcat cgtagttacg
    16261 tggtacggcc ttttgcacca gctgcgaaag cgtctgattt tgataacgca ccacggtttc
    16321 gagcgggaat ttcatcggtg ctgctggcgt ggactcctct ggcaatggtt gttctggcgt
    16381 catctgttta accatcatct ctgccagccc cagacctttg cccgccgtca tctgttgggc
    16441 aatctgctgg tcatacatac tggtatacag gcgagtgtgc tcgctgctga acaggccatc
    16501 ttttggtaaa gcgtcgcgca tgcttttcaa catcatctgc acgaacatcc cttccacctg
    16561 gcgggccacc ggacggatat ttgccgccgg atcttcgccc gctttcgcct taagttcgtt
    16621 gagcgattgt gcatcccagg ccgcacttgc cagtagtttg ctgtcgctga tcatcagatg
    16681 atttccagtt ttgcccgtag acatcccgca ctttgcattg attgcagtat ggacatcaga
    16741 tccatcggcg tagcgcccag cgcattgagc gcacgcacca cgttattgag gctggcgctg
    16801 gaacgtacgc tttgcagcga accgccgctc tggcgtaaat cgatctgcgt ttgtggagta
    16861 accacagtct gcccaccacc aaacggtgta tctggctggc tgacattggc ctggcgatta
    16921 actgttactg agagattccc ctgcgctacc gcgcagctgt cgagggtcac ttcgcgattc
    16981 atcaccaccg aaccagtgcg cgagttaatc actactttag cgtcctgcgg ggtgacatta
    17041 acctgcatat tctggatatc ggcaaggaaa cggacctggg aactgttgcc actcggtacg
    17101 cgcacctgaa ttgtccgcgc atctaacgcg gtggcgctgc catatccacg cacgcggttg
    17161 atggtgtcag cgatttgctg cgccatactg aaatcttcgt cgttaagttg caaattaagt
    17221 gtattcccga cgccaaactg gctgggcaat tcacgttcaa taaccgcacc attggtgatc
    17281 cgtccaccgt tcagttggtt cacctgaaca ctgctaccgc cagcggaggc ccctgcgccg
    17341 ccaaccagaa tattgccctg cgccagcgca tacacctgac tgtctacgcc cttaagcggt
    17401 gtcatcaata acgtaccacc acgcagactt ttggcatttc ccatggaaga aaccaccacg
    17461 tcaatggttt gcccctgacg tccaaacggt ggaagtgacg ctgtcaccat aaccgcagcg
    17521 acgtttttta gctgcatatt agtgcccgtc ggaacggtaa ttcccagctg tgaaagcatg
    17581 ttattaagcg tttgtgtggt aaacggcgtc tgagttgtct ggtcacctgt accatcaagc
    17641 cccaccacca ggccatagcc aatcagtgag ttttgcctta ccccctgaac actggtgaga
    17701 tcgcgaatac gctcagcctg agccgccgtg gtaaccagta aaagaattaa tgcagagaga
    17761 aatttaatca ccacagcctc acttacattg gcgacaggtt gaggaagaaa cgctgcaacc
    17821 aacccatatt ttgcgcttcg ttaatgtagc cattgcctac gtattcaata cgcgcatccg
    17881 ccacctgagt agacggtacg gtattgctgc cgctgatagt gcgtggatta accacgccag
    17941 agaagcgaat aaattcggta ccctgattaa tggcgatctg tttttcaccc accacatgca
    18001 gattgccgtt gaccagtacc tggtcaaccg tcaccgtcaa cgtgccgcta aaggtattac
    18061 tggcattggc cccgcctttt ccgttgaacg tgttaccacc ggaggcttcg acatcggcac
    18121 gagcgttacc aaacagcccc tgcaaatagc gcggcacagt atcaaagcca aaattagttt
    18181 taccgtcacg gctggcattc gcagaggagc ttttgctggc gctgacgttc tcctgcaaca
    18241 cgatggtcag cgtatcgcca atattgcgtg gtcgacgatc ttcaaacagc ggttgatagc
    18301 catagttaat cggctgagca gactggaaaa tagaaccgtt ggcgacgggc gtcggaccgg
    18361 gaaccggttg tgcactggtc gccccctgta ccagcggcgt ggagggtatc caggcgcagc
    18421 cggttagtga aagcaccaac aagctggaaa tggcataagt atgcgcagcg tttttttgca
    18481 tggctatcat cttcaaaaat cagtaaaccg gtgaatctgc caccggttat gccttagagt
    18541 tgcgtcagtt tttgcagcat ctgatcggtg gtggataccg ctttactgtt gatttcgtaa
    18601 gcgcgttgca cctgaatcat attgaccagt tcttccgcca cgttgacgtt agacgtttca
    18661 acataccctt gatacagcag tcccgcgccg ttcaggcccg gcgtgctttc gttcggtgca
    18721 ccggaggatt gcgtttcggt gtagaggttt tcgccaatac tctccagtcc ggtatcattc
    18781 ataaaggtgg tgagattgag ctgcccaacc tgaaccggag ctgcctggcc ttgttgtgtt
    18841 acgctgacca cgccatcacg accgatggtg atacttagcg cattcgccgg aatggtgatc
    18901 gctggctgca cctgaaaacc acctgccgtc accagctgcc cgttctgatc cacctagaaa
    18961 gagccgtcac gggtataggc tgatgagcca tctggcaaca tcacctggaa aaagccctgc
    19021 cctttaatcg cgacatcttt gctgttgttg gtctgcgaca gattgccctg actgtgtaag
    19081 cgctcagtag caaccggacg cacccccgtg ccgatttgta acccggaggg taaggtggtt
    19141 tgttcggaag actgtgcccc cggctggcga atggtttgat acagcagatc ttcaaacacc
    19201 gcgcgctgac gcttaaaacc gttggtactg acgtttgcca ggttgttggc aatgacgtcc
    19261 atattggttt gctgggcatc caggcccgtt ttggcgatcc ataatgaact tatcataaaa
    19321 tgtcctgtag tggattagtg acaaagtgca tctgtcttct tgccggatgc ggcgtaaacg
    19381 ccttatccgg cctacaagag ctcgcgaaat caataaattg cgattgcccc atagacccga
    19441 taaacttgcg catcgggcaa cttatacttg tcatgtatcc tttcaattaa ctcatcgaca
    19501 gcagttggtt ggctcggcct gcgttatcat cgacgctgct gatcaccttc atctgcattt
    19561 caaaacgccg cgcgctggca atcatgtcgc tcattgccgc aacggcattg acgttactgc
    19621 cttccagaac acccgacatc acacgcaagg ttggatctgc ctgcagtacc ggcccacgcg
    19681 tggtctgggt ttctgcgctt aaacgaaaaa tgccgtcgtc accgcgctgg acttcgctgc
    19741 ccgtggcttt caccagtttc agacgcccca ctggcgcaac cgtatttgcc ggatcgcccg
    19801 gattcagcgc cgagattgtg ccatcggcag caatagtgat ttccgcccct tccggcacag
    19861 caattggccc agcctcgcct atcaccggat gtccctgaat tgtcagttgc ccggtgggat
    19921 caacctgaat gctgccatta cgcgtatacc cttcgctacc gtcagcggtc tgcacggcca
    19981 gccagccatc ctgctgcaac gcaacgtcca gcgggcgcga ggtgtaatcc atcttgccgg
    20041 gcgtcatgtc tgcacccggc gttgacgccg ttaccagcgt gcgcgtaggc agtgaaagcc
    20101 cttccaccgg cactgcgcgc aaggcgttta actgcgcgcg aaaaccgggc gttgaggcat
    20161 tggccagatt actggcagtt accgcctgtt gattcaacgt ctggctggct gctcccatcg
    20221 cggtataaat tgcgtgatcc attgagctat cccgtcagcg attagcgtaa gttaaccagc
    20281 gtgttgagga tctggtcctg ggttttgatg gtctgggcgt tagactgata gttacgctga
    20341 gcaacgatca tattgaccag ttctttactg agatcgacgt tggacgcttc cagcgcaccg
    20401 ttggtcaggg tgccaaagtt gcccgtcccg gctgtcccca acagcgccac gccagaagat
    20461 tgcgtcgcag accagacgtt gtcgccttcg gatgccagac cttcgttgtt ggcaaagttc
    20521 gccagtacaa tctgccccag cagttgggtt tgttcgttgg aatagttgcc gacaaccgta
    20581 ccgtcatcat tgatttgata actcaccaga tcgcccggtt tgtagccgtt ctgggtggtt
    20641 gccacaatgt tgttagcgcc ggtattttgc tgcatggagt tgaggaagct caggctaaac
    20701 gtggcgggtt ctgcaccgtt aattgcgcca gtggaaatat tcgctgttgg atctgaggtc
    20761 agaacgccat tggcattaaa caccagcgtc attgcaggct cagctgtacc tgttggatca
    20821 ctgctatcct gggtgtagac ctgccagtta ttatccccgg tcttcacaaa gtagacgctc
    20881 atgtcatgag cattaccctg actatcgaaa acagtcaccg aacctttttt gttatagcta
    20941 tccgcattgc tggcatcaaa tgcgttaaca gagggaagcg gatcactgga attcaggttg
    21001 atctgcatcg acgccgtggt ggtagttttc gctgccatca gggtattcgg aatcgaaata
    21061 ttggtcggat tcgccccttg ctgaatagtc ggcggcgtac cggttgccgg gtaacccgtc
    21121 agctgtaaac cttgcatatt caccaggtta cggttttcat ccagcttaaa ttgtccgtta
    21181 cggctgtaga acaccgaacc gttgctgtct accagacgga aaaaaccgtt ctggctgata
    21241 gcaacgtcca gacctcgccc ggtgttggtg gtcgtgccat cggtaaagtc ctgagtgata
    21301 ccagcaactt ttacccccag tcccactttc gaaccggcaa acatatcggc aaaagaggcc
    21361 gtgcctgatt taaagccgta ggtggcagag ttggcgatat tgttgccaat aacatcgagg
    21421 ttggtggcgg cagcgtttaa tccgctaacc gcttgagaaa aggccatgac tgactcctga
    21481 aatgtgaagg cttaaattat ctgccgtact tcgtcgaggg tggtggtgcc gtaagtgccg
    21541 agatccagcg tattaccgtt gttgccgcgg atcacaccct gcaccagagc aaactgcagc
    21601 ggctgggcaa ccagttgtgt accaccgtta ctggcgctaa tcgctacgtt gtaagaaccg
    21661 ttcggcgcag tggtgccgtc agtcaacgta ccgtcccagg tgaaactgtg gactccggcg
    21721 gtcagttcac caatatcaat ggtacggaca accgcaccgt ttttatcggt gatggtggcc
    21781 gtcactttgt ctgccgcctg ttgcagctca acaccaaacg gcgtggtcgt ggtcacagcc
    21841 ccttcttcac tgccggttcc cgcaagaaca gtggtgccgg ggatcatcac gccgtgaccg
    21901 atcaggttac tggcctgtaa cgactggctg ttatcaatct gtccggaaat agatccgagc
    21961 gtggtattga gtttttcaat cccgctgacc gtgctgattt gagccaattg cgacgtcagc
    22021 tcgttgtttt ccattggatt ggtcgggtcc tggtttttca gctgcgccac caacaaagtc
    22081 agaaaactgc tttgtaaatc tgcggcgttg ctgcccgtga gcgaactact gctggtggtg
    22141 ctgacgccgg tatttgtcgg atcggtggtg gttaccgcaa tggacatagt tttctccttt
    22201 attgaccgag cgtaagggtt ttcagcatca tgcttttcac cgtgttgagc acttcaacat
    22261 tggcctgata gctgcgtgac gccgacatgg tgttaaccat ctctccgacg acatcaacgt
    22321 ttggcatttt cacgtagccc tttgcatccg ccagcggatt acccggttca taaaccagtt
    22381 tgtccggggc ctgactttct ataacatcgg caacctttac gccgcctgtc gcagcacctg
    22441 gcgcagcgtt aacctgaaat accacctgtt ttgcccgata tggctgtcca tcgggaccgg
    22501 tcacgctatc agcattcgcc agattactgg ccgccacgtt caggcgctgg gactgagcag
    22561 ttaacgccga cccggcgata tcaaaaatat tcagcagtgc catccgttaa tttccgctct
    22621 gtaaaacgtt catcatgcct ttgatttgcc cgctcaacgc gctaaggctc atctggtatt
    22681 gcaggctgtt atcggcaaac tgggtgcgtt cgcgatccat atcgacagta ttaccatcca
    22741 gcgagggctg gtccggaata cggtattgca gttctgcggt aggaggcgtc agcgcctgcg
    22801 ccggaatgtg ttgcgttgag gtcatcgtca gtgcaaccac gcttgttgca tcccgtccac
    22861 gttgcatgac ttttttaagt tcactggcaa aatcgatatc gcgcgcctga taaccagggg
    22921 tatcggcatt ggcgatgttt gctgccagca cttcctgacg ctgggcgcgc agattgagcg
    22981 cctcttgttg aaaacgtaag gcggcgtcga gcttatcgag catatctcct ccgcaggtat
    23041 caaaattcgg ccatcagctt aaatgccttt acgcgcgcgt tatcggcgga ataaacgcaa
    23101 aatgggtcgc tatttatgcc gttgatggtc attgcggaca ggtacaattc acgttgtaga
    23161 aatggctggg gggcgaaaat gctggcaata aaacgtagcg tggcgatcat cgcgatactg
    23221 ttcagtccgt taagtgcggc gagcaatctc acatcgcaat tgcacacctt ttttagcgcc
    23281 cagctcgcgg gggtaagtga tgaggttcgt gtttctattc gtacagcgcc caatctacta
    23341 ccgccgtgcg agcagccatt gctttcgatg agcaataatt cccgcctgtg gggcaatgtg
    23401 aatgtgttgg cacgctgcgg taacgacaaa cgatatttac aggttaatgt gcaggccaca
    23461 ggaaattatg tggttgccgc aatgcccatt gtgcggggag gaaagctgga agctggcaat
    23521 gtcaaactga aacgcggacg gctggacacc ttgccaccgc gtacggtgct ggatatcaat
    23581 caacttgttg atgccgttag cctgcgcgat ctatcacccg atcaacctat ccagttaacc
    23641 cagtttcgcc aggcatggcg ggtaaaagcg ggacaacgcg tcaacgtgat cgccagcggt
    23701 gatgggttta gcgccaacgc agaaggtcag gcgctgaaca atgctgccgt cgcacagaat
    23761 gcgcgggtgc gcatggtatc gggacaagtg gtcagcggcg ttgttgatgc agatgggaat
    23821 attcttataa acctgtaagc tgttaaagat tacccgtccc ttgccgataa ataagcaaca
    23881 catgataaaa gcgccctcaa tgaggaataa accatgagta ttgatcgcac ttcgcctctg
    23941 aagcccgtaa gcaccgttca accgcgcgaa accactgacg cgccggtaac gaacacccgg
    24001 gcggcaaaaa caaccgcctc caccagcacc agtgtgacgt taagcgacgc gcaagcaaaa
    24061 ctgatgcaac ccggcagcag tgatatcaat cttgaacgtg tcgaagcgtt aaaactagcg
    24121 attcgtaacg gtgaactaaa aatggacacc ggcaaaattg ccgatgcgct gatcaacgaa
    24181 gcgcagcaag acttgcagag taactgatcg tatgacgcgt cttgcagaga tcctcgacca
    24241 gatgtccgct gtgcttaacg atctcaaaac ggtaatggat caagagcagc aacatctctc
    24301 tatggggcag atcaacggca gccagttgca atggattaca gaacaaaaaa gctctctgct
    24361 ggcgacgctg gattacctcg aacagttacg caggaaagaa cccaatgcag caaatagcgt
    24421 tgatattagt caacgctggc aggaaattac tgtgaaaacg cagcaactac gccaaatgaa
    24481 tcaacataac ggctggttac tggaaggaca gattgagcgc aatcaacagg cgctggaaat
    24541 gttgaaaccg catcaggaac cgacgctata tggagcgaac ggtcagacct caacaaccca
    24601 tcgcggcagt aaaaagattt cgatctgaag attatctccg gcctgcactg caggccggaa
    24661 tgccttttta caccgtccgg cgggcaaact ctttaacttt gaagcccagt accgccagtg
    24721 ctgcaaagta cgcggcaatc cccgccagca cgaccgccat taaacgcagt aaacgccagg
    24781 gcatggtacc caatgaccac tccggcatga tatgtaacat acctaaaagc acgccagaca
    24841 tcaccagtac cgccaccacc agacgcaaca gaaacgccat ccagccgggt tgcggggtaa
    24901 agattttctg cttacgcaac tgccagtaaa gcagcgaagc attcagacac gccgccagac
    24961 caatagaaag tgacagcccg gcatgtttca acggaccgat aaacgccagg ttcatcaatt
    25021 gcgttaaaat cagcgtaacg atggcaattt tcactggcgt tttaatgtcc tggcgggaat
    25081 aaaagccagg agccaacact ttcactacaa tcaggccaat caaacccacc gagtaggcaa
    25141 ttaacgcccg ctgggtcatc agcgcatcaa aggcggtaaa tttaccgtac tggaacagcg
    25201 aaacggttaa cggacctgaa agaatgccca acgcaaccgc actcggcagc gccaacagga
    25261 aacaaagacg cagcccccag tccatcaaac ggttgtattc atcatgattg ccactggcaa
    25321 aacttttcga cagcgacggc agcaaaatgg tgccaagcgc cacgcccagt acaccggacg
    25381 gaaactccat taagcggtcg gcgtaataca tccaagacac cgaaccggaa gcaagaaacg
    25441 aggcaaaaat ggtgttgatg attaaggaga tctggctcac agagacgcca aggatcgccg
    25501 gtcccatctg tttcaccacg cgcattgctc cggcatcgtg gaagttaatg cgcggcagga
    25561 ccagcatgcc gatcttcttc aggtgcggta gctgatacac cagctgcagg atgccgccga
    25621 ccgtaacagc ccacgccagc gccagcaccg gtgggttaaa gtacggtgcg gcaaacagcg
    25681 cgaaaccaat catgctgata ttaagcagtg ttggagcaaa cgccggaatc gagaagcggt
    25741 tccacgtatt cagaatcgct cccaccagcg acgccaggga gatcagcaag atatagggaa
    25801 aggttatctt tagtagctgg ctggtcaggg caaatttgtc ggcagtgtca gcgaagcctg
    25861 gcgcggtcac catgatcacc cacggtgcag cgagcatgcc agcgaccgtc acaaccgcca
    25921 gcgcgagtgt cagcaggcca gaaacataag agacaaagac ccgcgtggcg tcttcaccct
    25981 gcttgctttt atattctgcc agaatcggta caaatgcctg ggaaaatgcc ccttcggcaa
    26041 agatacggcg caacaagtta ggaagtttaa aagcgacgaa aaaggcgtcg gttgccatcc
    26101 ctgcgccaaa gattctggcg acaattgcgt cacgtgcgaa gccaagcaca cgcgaaaaca
    26161 tggtcatcga gctgacggcg gccagcgatt ttaataaatt catcggtgtt ctaatccaga
    26221 cccacgggca aaacgcctgc aaagcaggcg ctgaaagagg cgaatagtct acctggatta
    26281 tggtgaattg ctaccgccag atgttacagg gtttattcac tcatcgcatc gcgccagatc
    26341 ttgtcaacga tacgttgcgc ccgcactgcc tgttcgccgg cggtttgcgg aactgtctga
    26401 ttttgcacgc attcaatgaa gtgccgcgca cagccgacaa atccacgttg ctcaagcgta
    26461 ctctgccaac caggaatcgg tttatgcact acgccctgcc cgcgctcctc acgccattcg
    26521 cgcatatccg taatgtcgat gagcgcaccg tcagtcacgg cctgcacggt ttcacgctgg
    26581 cttccggcac ggcgatgcat acaggtggtg atctgcaaag gaccggccga aaaatggtgc
    26641 tcggcaaaca gcatttcgcc agcgtcgtta gtcagtagcg taccgccatc cagagaggct
    26701 ttgccgcccg acaaccacag cgcggtatcc accacatgca gataatcatc cagcaacgtg
    26761 aaataaagat cgtgtggccc gacgctattg ctacgatgtt tatccattct tagcgaggct
    26821 gcggtggcga gttgcgtttt taactctccg tagagtggtg cgaaacgacg gttaaaaccg
    26881 accatcaacg tcagtttttt ccgcgccgcc agttccacca accgttcagc atcgcgcaga
    26941 ttttctgcca gcggtttatc gacacagaca tgtacccccg cattgagtaa cgtactgacc
    27001 acgtcaaagt ggctggcggt gctggaatgc acaaaaaccg catcgcaact ggcggcaagg
    27061 ctggataacg aatcggcata aggaatgcgc cagctttcac aaattggcag ggctttcgcg
    27121 cgcgtaggcg accaggctcc ttgtaacatc cagtcagacg ctgccgccag caccggtaac
    27181 cacgcttttt gcgcaatgcc gcctaatccc actacgccga tacgtaattt tttcacttta
    27241 atctcccaga tgggcaagca acgaatcaag acgctgtttc agttctgcca cttcgatttc
    27301 cagggcttcg acgcgagcct gtaaatcacc gtcaaccgcg ttcgacatat ccatcaccgc
    27361 cggctgatct tcaacctcac cactgaaaag atgcatgtag cggctttcac gtttacccgg
    27421 ttcgcgggcc agacgcacca caaaaggacc atcttcgcga tttgccagtt gttccagcgt
    27481 cgactccact tccgccatat cgctgaattc atacattcgc gcggcgcggc tgcgcagttc
    27541 acccggcgtc tgggcaccac gcaataacaa cgtggtgatt aacgccactt ctgcggcgct
    27601 cagtttcaga tcgccaaatt ctgaattaca aaaacgttgc tcatatttgg tgacccgatt
    27661 accaaaaccg ctcactgtgc gtagataatg acgtttgacc agattatcca gctgttcctg
    27721 cacttcggat tcactcagat tcatgaccgg ttcacggttc gttttctgat tacaggccgt
    27781 gactacacca ttgactgaga gcgggtattg ctccggcgtg gtcacctgct tttccagcaa
    27841 gcaaccaatc acgcgagctt caagggcagt aagttgatat ttcatgagat tctccttagc
    27901 ggccgggcgt ccagtctggg gtagttaatg ccgtcagtac gtgatcgcgc cattgtccat
    27961 caatcaacag atagtctttc gcatagcctt ctttttcaaa acccagtcgc gccagtaaat
    28021 caccgctgcg tttattgtgc ggcatataat tagccataat gcgatgaata tgttgggtgc
    28081 gctgcatata acgaatggct gcggtcaggg cttcaaacat gagtcctttg ccctgccatt
    28141 tttgcccaat cgaataaccg agatagcagg catgaaaaga gccacgaaca acattggaaa
    28201 aattggcaac gccaataatc tctttttcgt ccgggtcgaa taagccaaag tagaaagctg
    28261 aaccttgttt atgaaattcg ttaatcatcc ccagcctggc ctgccagcct gatggataac
    28321 agtggctttc gtcgcgcact ggctcccagg gcttgaggaa atggcgattc tctgcgtaat
    28381 aatccgcaag acgccaggca tcacgatcat gcaccagacg cacgaccagt cggtctgtgg
    28441 ttaagcgcac ttttggcacg ttactgcgat agccaaacat tctataccta ctccttcccg
    28501 taacgtcttt aatacgctgt aagtattact atacctgcgc aaccagaccc tgtgaaaagt
    28561 ccagaatgtc attttctgca ttattcacat atttgataaa aactctctat caaagcgacg
    28621 ttttgataaa aaaatattgt ccccgaagtg atgggaaaag tcttagcggc aatgcagaat
    28681 agaaagtgct catcttttat ttcccgggag gggaaatgtc ccgcgtatcg caggcgagga
    28741 acctgggtaa atatttcctg ctcatcgata atatgctggt cgtgctgggg ttctttgttg
    28801 tcttcccgct gatctctatc cgcttcgttg atcaaatggg ctgggccgcc gtcatggtcg
    28861 gtattgccct cggcctacgc cagtttattc agcaaggtct gggtattttc ggcggagcaa
    28921 ttgccgaccg ctttggtgcc aaaccgatga ttgttaccgg tatgctgatg cgtgccgccg
    28981 gattcgccac aatgggtatc gcccacgaac cgtggctatt gtggttttca tgcctgcttt
    29041 cgggactcgg cggcacgttg tttgatcctc cgcgttcggc gctggtggtg aaattaatcc
    29101 gtccacagca gcgtggtcgt tttttctcgc tgttgatgat gcaggacagt gccggtgcgg
    29161 tcattggcgc attgttgggg agctggctgt tgcaatacga ctttcgcctg gtctgcgcca
    29221 caggggcagt tttgtttgtg ctgtgtgcag cgttcaatgc gtggttgtta ccggcatgga
    29281 aactctccac cgtacgcacg cccgttcgcg aaggcatgac ccgcgtgatg cgcgacaagc
    29341 gttttgtcac ctatgtactg acgctggcgg gttactacat gctggctgta caagtgatgc
    29401 tgatgctgcc aattatggtc aacgacgtgg cgggcgcgcc ctctgccgtt aaatggatgt
    29461 atgccattga agcgtgtctg tcgttaacgt tgctctaccc tatcgcccgc tggagtgaaa
    29521 agcattttcg tctggaacac cggttgatgg ctgggctatt gataatgtca ttaagcatga
    29581 tgccggtggg catggtcagc ggtctgcaac aacttttcac cctgatttgt ctgttttata
    29641 tcgggtcaat cattgccgag cctgcgcgtg aaaccttaag tgcttcgctg gcggacgcaa
    29701 gagctcgcgg cagctatatg gggtttagcc gtctgggtct ggcgattggc ggcgctattg
    29761 gttacatcgg tggcggctgg ctgtttgacc tgggcaaatc ggcgcaccag ccagagcttc
    29821 cgtggatgat gctgggcatt attggcacct tcactttcct tgcgctgggt tggcagttta
    29881 gccagaaacg cgccgcgcgt cgtttgcttg aacgcgacgc ctgatttgat actcccccct
    29941 gactcctcca tactgaaagt gaactgacag ccaaatggag gagtcacgtg aagctataca
    30001 tttacgatca ctgcccttac tgcctcaaag cccgcatgat tttcggcctg aaaaatatcc
    30061 ccgtcgaatt acatgttctg ctcaacgacg acgcagaaac acccacccgg atggtcggtc
    30121 aaaaacaggt tcccattctg caaaaagatg acagccgcta tatgccagaa agcatggaca
    30181 tcgttcacta tgtcgataaa ctcgacggca aaccgttact gaccggcaaa cgttcccctg
    30241 ccattgaaga gtggctgcgc aaggtcaatg gctacgccaa caaactgctg ttgccgcgtt
    30301 ttgccaaatc ggcatttgat gagttttcta ctcctgccgc gcgcaaatat ttcgtcgaca
    30361 agaaagaggc cagcgcgggt aattttgccg acctgctggc ccactctgac ggtctgatta
    30421 agaatatcag cgatgattta cgtgcgctgg acaaactgat cgtcaaaccg aacgccgtga
    30481 atggcgaact ttcggaagat gatattcagc tatttccgct gctgcgtaat ctgacgctgg
    30541 tagccggaat taactggcca agccgcgttg ctgattaccg cgataatatg gcgaaacaga
    30601 cacaaatcaa tttgttatca tcaatggcga tttaatcctg acccgccgcg gcagcctgta
    30661 ttcgcgggtt gccgccgttt tcagtttttt ctgctggcgc gtttatcgct ggtgacacat
    30721 gctgcgttgt gtcaggatat tgacgcagat ttcatgtcga ggactaccat gaacaagttt
    30781 ttatttgccg ctgcattgat cgtcagtggc ctgctcgttg gctgtaatca actcacccaa
    30841 tacaccatca ccgaacaaga aattaaccag tcgcttgcga aacataataa tttctcaaaa
    30901 gatatcggtt tacccggcgt ggctgacgcc catattgttc tgacaaacct gaccagccaa
    30961 attggtcgcg aagagccgaa taaggttacc ctaaccggag acgccaatct ggacatgaac
    31021 tccctgttcg gtagccagaa agcgaccatg aaactgaagc tgaaagcgct gcccgtgttt
    31081 gataaagaaa aaggcgctat cttcctgaaa gagatggaag tggtcgatgc gacggtacaa
    31141 ccggaaaaaa tgcaaacggt gatgcaaacg ttgcttccct atttaaacca ggcattacgc
    31201 aattacttta accagcaacc tgcttacgtc ctgcgcgaag atggcagcca gggcgaagca
    31261 atggcgaaga aactggcgaa aggcattgaa gtgaagccag gcgaaattgt cattccgttt
    31321 actgattaat cacgagggcg cattcgcgcc ctttattttt cgtgcaaagg aaaacgtttc
    31381 cgcttatcct ttgtgtccgg caaaaacatc ccttcagccg gagcatagag attaatgact
    31441 gcaccatccc aggtattaaa gatccgccgc ccagacgact ggcaccttca cctccgcgat
    31501 ggcgacatgt taaaaactgt cgtgccgtat accagcgaaa tttatggacg ggctatcgta
    31561 atgcccaatc tggctccgcc cgtgaccacc gttgaggctg ccgtggcgta tcgccagcgc
    31621 attcttgacg ccgtacctgc cgggcatgat ttcaccccat tgatgacctg ttatttaaca
    31681 gattcgctgg atcctaatga gctggagcgc ggatttaacg aaggcgtgtt caccgctgca
    31741 aaactttacc cggcaaacgc aaccactaac tccagtcacg gcgtgacgtc agttgacgca
    31801 atcatgccgg tattagagcg catggaaaaa atcggtatgc cgctactggt gcatggtgaa
    31861 gtgacacatg cagatatcga catttttgat cgtgaagcgc gctttataga aagcgtgatg
    31921 gaaccgctac gccagcgtct gactgcgctg aaagtcgttt ttgagcacat caccaccaaa
    31981 gacgctgccg actatgtccg tgacggaaat gaacggctgg ctgccaccat cactccgcag
    32041 catctgatgt ttaaccgcaa ccatatgctg gttggtggcg tgcgtccgca cctgtattgt
    32101 ctacccatcc tcaaacgcaa tatccaccaa caggcattgc gtgaactggt cgccagcggt
    32161 tttaatcgcg tattcctcgg tacggattct gcgccgcatg cacgtcatcg caaagagagc
    32221 agctgcggct gcgcgggctg cttcaacgcc ccaaccgcgc tgggcagtta cgctaccgtc
    32281 tttgaagaga tgaatgctct gcagcacttt gaagcattct gttctgtaaa cggcccacag
    32341 ttctatggct tgccggtcaa cgacacattc atcgaactgg tccgcaaaga gcaacaggtt
    32401 gctgaaagca tcgcattgac tgatgacacg ctggtgccat tcctcgccgg ggaaacggta
    32461 cgctggtccg ttaaacaata aaaatcgtag cttcctgttg tcattaggtt attttaactg
    32521 tataaataac cagtatattc aacagggggc tattatgcga attgaagtca ccatagcgaa
    32581 aacttctcca ttgccagctg gggctattga cgccctggct ggcgaacttt cccgccgtat
    32641 tcagtatgcg tttcctgata atgaaggcca cgtatcggta cgttatgccg cagcgaataa
    32701 tttatcggtt attggcgcga caaaagaaga taaacagcgc attagcgaaa ttctccagga
    32761 aacgtgggaa agcgccgatg actggtttgt cagcgaataa tatgcagtga tttttgtttt
    32821 tgccgggtcg ccccggcttt tttattttac ttgcgaattg tgagtacaaa atttctacac
    32881 ttcctcaaaa taattgcaca atatgctaaa aaaatatgaa tgacatgctg ttttattgtt
    32941 catataaatc ttaaaagcaa aaatttatct gttcccccct ttaaaaattc ggtgaatacc
    33001 cttacttatt ggtatactga agttgcttca gataaaaacg cattgaacct cgaataacgt
    33061 tgtctagtaa cacgaattag ggggccatga tggaaaaaaa taatgaagtc attcagactc
    33121 atccgctcgt agggtgggac atcagcaccg ttgatagcta tgatgcgctg atgttgcgtt
    33181 tgcactacca gaccccaaat aagtccgagc aggaagggac tgaagttggt cagacgctct
    33241 ggttaaccac tgatgttgcc agacaattta tttcgatatt agaagcagga atcgccaaaa
    33301 ttgaatccgg tgatttcccg gtaaacgagt atcggcgtca ttaacatgac atgaccatcc
    33361 acccaaaagg cacctcaccg gtgcctttac catttctggg ttgtatatct gcattcagtt
    33421 aattactctg cttaaaccat gagagcagag agaaaaaaat gaaatacgat ctcatcatta
    33481 ttggcagcgg ttccgtaggc gctgccgccg ggtattatgc aacccgtgcc ggtttaaacg
    33541 tgctaatgac cgacgcccat atgccaccgc atcaacacgg cagccaccac ggcgatacgc
    33601 gattaattcg ccatgcttat ggtgaaggcg aaaagtatgt cccgctggtt ctccgcgcgc
    33661 aaacgctgtg ggatgaactc tcccgccaca acgaagaaga tcccattttt gtacgctctg
    33721 gcgtcattaa ccttggcccg gctgattcca catttctcgc caacgtcgcc cacagcgccg
    33781 aacaatggca actcaacgtt gaaaagctcg atgcgcaagg gattatggcc cgctggccag
    33841 aaatacgcgt cccggacaac tacatcggct tatttgagac tgattccggc tttttacgca
    33901 gcgaactggc gattaaaacc tggatccaac tggcgaagga agcgggctgt gcgcagctgt
    33961 tcaactgccc ggtcaccgca attcgtcatg acgatgatgg cgtaactatt gaaacggctg
    34021 acggtgagta tcaggcgaaa aaagcgattg tctgcgcggg aacatgggta aaagacctgc
    34081 tcccggagtt gcctgtccag cccgtacgca aagtatttgc ctggtatcag gccgatgggc
    34141 gctatagcgt gaagaataaa ttcccggcgt ttaccggtga acttcccaat ggcgatcaat
    34201 attatggttt tccggcagaa aacgacgcgt tgaagattgg caaacataac ggaggccagg
    34261 ttatccattc agcggatgaa cgtgttccgt ttgcggaagt ggtcagcgat ggttcggaag
    34321 ccttcccgtt cttgcgcaat gtattgccgg gtatcggttg ctgcctgtac ggcgccgcct
    34381 gcacctatga taattcgcct gacgaagatt ttattatcga taccctaccc ggccacgata
    34441 atacactgct cattaccggc ctgagtgggc acggttttaa atttgcgtca gttttagggg
    34501 aaatagctgc cgattttgcg caagacaaaa aaagcgattt tgatttgacg ccattcaggc
    34561 tttcccgctt ccaataatca tcattacggc ctccagaatg aggccgtttt tacaggagta
    34621 tttaatgcgt cggctgttgc actatctcat caataatatt cgcgagcatc tgatgcttta
    34681 tctttttctc tggggattgt tggcaattat ggatctgatc tatgtgtatt atttttagtt
    34741 ttccgcactg acataaactg acaaatattt aatccccctt tcatttagcg tgaaaaattg
    34801 aatgagatta ttcctgcttc aacagcactc accagacatc tctgatgctg aacccactaa
    34861 acttatgaag attctttcat tattaattca aaattattga aataaaaata aatatcattc
    34921 aaattgttat ttttgtgttg caattcattc tccgacacgc cattttcttt ctctcgcaaa
    34981 gtcgttttgg atgcacttat gtcattcaca aatacccctg aacgctatgg tgttatatct
    35041 gcggccttcc attggctaag cgcaattatt gtctacggca tgtttgcttt aggtctgtgg
    35101 atggtcacgc tcagttatta cgatggctgg tatcaccagg cacccgaact gcataaaagc
    35161 atcggtattt tgttaatgat ggggctggtt attcgcgttc tgtggcgcgt catatctcca
    35221 ccgccgggtc cgctgcagag ttattcgccc atgactcgtc ttggcgcaaa ggctggacat
    35281 ctcgcattgt acttattgct gttcgccatc ggcatcagcg gctatctgat ctcaactgcc
    35341 gatggtaaac cgatcagcgt ttttggctgg tttgacgtcc ccgcgaccct cgccgacgct
    35401 ggcgcacagg ctgactttgc cggtgccctg catttttggc tcgcgtggag cgtcgtagta
    35461 ctgtccgtta tgcacggatt tatggccctg aagcatcatt tcatcgataa agacgacact
    35521 ctgaagcgca tgctgggaaa gtcgtcatct gactatggag tataaaaaat gaaaaaaagc
    35581 ctgcttggtt taaccttcgc ttccctgatg ttctctgccg gttcagcggt tgccgccgat
    35641 tacaaaattg acaaagaagg tcagcacgcc tttgttaact tccgcataca gcaccttggc
    35701 tatagctggt tatacggcac ctttaaagat ttcgacggta cttttacctt tgatgaaaaa
    35761 aatccagctg ccgataaagt gaatgtgaca attaacacca ccagcgtcga tactaatcac
    35821 gccgaacgcg ataaacatct tcgcagtgca gatttcctca atactgcaaa atatccacag
    35881 gcaacattca cctccaccag tgtgaagaaa gacggtgacg aactggatat taccggcgat
    35941 ctgacgctga atggcgtaac caaacccgtc acgctggaag cgaaattaat tggtcagggc
    36001 gacgacccat ggggtggtaa acgtgcaggc ttcgaggccg aaggcaaaat taagctcaag
    36061 gacttcaata tcaaaacaga tttaggtcca gcttctcagg aagtggatct gattatttca
    36121 gtggaaggcg tacagcagaa gtaagcgtga aatgttgccg gatggcacgc atccggcaat
    36181 gatatttatt ctgttggatc aggaatgccc agtgttgtat tcagacgtcc acgagactta
    36241 ttaaagatct tattgccatt ttcacgtcct gcccgacggc gtcgctgttc ttcagacggt
    36301 aacgcgcttt cttcgcagca aatctcacta caacaacctt tgtatttttc cgcacatact
    36361 ggacactgaa taaaaagcag gtggcagcca tcatttttac agttggtatg gctgtcgcac
    36421 ggcgcaccgc actggtggca atgcgcgata atctcatcag agatacgttc gcccatccgc
    36481 tcgtcaaaaa caaaattttt gccaataaaa cgtaccggta aaccctgctc gcgcgccttg
    36541 cgggcgtatt caataattcc gccctcgata tgccagactt tattgaatcc gttatgtttc
    36601 atccaggcac tggccttttc acaacgaatg ccgccggtgc agtacataac gattttttta
    36661 tctttatgtg cctgcatcat ctcgactgct tttggcagct gctcacggaa ggtatctgcc
    36721 ggaatttcca gcgcgttttc aaagtgcccc acttcatact catagtggtt acgcatgtcg
    36781 ataaacaatg catcgggatc gtcaagcatg gcgttcactt ccgccgcttg cagatactca
    36841 cccacattgc tggcatcaaa gtgaggatcg ttaataccgt cggcaacgat acgatcacgg
    36901 accttcatgc gcagtaccca gaaggatttc ccgtcatcat ccaacgcgat attcaggcgt
    36961 aagccctcta aagccgggtc gaaggcataa agctgcgcgc gaaatgtttc aacattgctc
    37021 gcaggtacgc tgatttgcgc gttaatgccc tcatgcgcca gatacactcg cccaaaaaca
    37081 tccagcgcgg taaacagctg atataaagcg tcacgggtcg ccttaggatc ggcgatgtgg
    37141 aagtacttat aaaacgaaat ggtggttcgc ggttcgctct cagccaacat tttggctttt
    37201 aacgcgtcgt tggaaatgcg gttgtgtaac actggcatgg tgtacggttc ctgcgagatg
    37261 ggaaagtaaa aatccgcggc atgatatagc aattatcgat aattaacatc cacacatttt
    37321 acgctacatt tgcgcattaa aaattatttg ttatttacaa gcgcggcaat ttcgcccagt
    37381 cttcagccac aaattttggt tgcgggcgaa aaaatgcgac aatacataca attgcccgaa
    37441 taggttgaaa aacaggattg atatgacgaa tctacccaag ttctccaccg cactgcttca
    37501 tccgcgttat tggttaacct ggttgggtat tggcgtactt tggttagtcg tgcaattgcc
    37561 ctacccggtt atctaccgcc tcggttgtgg attaggaaaa cttgcgttac gttttatgaa
    37621 acgacgcgca aaaattgtgc atcgcaacct ggaactgtgc ttcccggaaa tgagcgaaca
    37681 agaacgccgt aaaatggtgg tgaagaattt cgaatccgtt ggcatgggac tgatggaaac
    37741 tggcatggcg tggttctggc cggaccgcag aatcgcccgc tggacggaag tgatcggcat
    37801 ggaacacatt cgtgacgtgc aggcgcaaaa acgcggcatc ctgttagttg gcatccattt
    37861 tctgacactg gagcttggtg cgcggcagtt tggtatgcag gaaccgggga ttggcgttta
    37921 ccgcccgaac gataatccac tgattgactg gctacaaacc tggggccgtt tgcgctcaaa
    37981 taaatcgatg ctcgaccgca aagatttaaa aggcatgatt aaagccctga aaaaaggcga
    38041 agtggtctgg tacgcaccgg atcatgatta cggcccgcgc tcaagcgttt tcgtcccgtt
    38101 gtttgccgtt gagcaggctg cgaccacaac cggaacctgg atgctggcac ggatgtccgg
    38161 cgcatgtctg gtgcccttcg ttccacgccg taagccagat ggcaaagggt atcagttgat
    38221 tatgctgccg ccagagtgtt ctccgccgct ggatgatgcc gaaactaccg ccgcgtggat
    38281 gaacaaagtg gtcgaaaaat gcatcatgat ggcaccagag cagtatatgt ggttacaccg
    38341 tcgctttaaa acacgcccgg aaggcgttcc ttcacgctat taaatctccc atgccggatg
    38401 cttcagaagg gtatccggca ttaccacagc aaatccccct gatttagcga taaaagctct
    38461 ctggattgcg ccccctggaa gtcgggcgca taattagtgt gcttatcttt tcttcttatg
    38521 ttcaccgcgc ctggcgcacc aacagcggat tgctatgtca ccctgtgaaa atgacacccc
    38581 tataaactgg aaacgaaacc tgatcgtcgc ctggctaggc tgttttctta ccggtgccgc
    38641 cttcagcctg gtaatgccct tcttacccct ctacgttgag cagcttggcg ttaccggcca
    38701 ctccgccctg aatatgtggt ccggtattgt cttcagcatt acatttttat tttcggccat
    38761 cgcctcaccg ttttggggtg gactcgccga ccgtaaaggc cgaaaactca tgctattacg
    38821 ctctgccctc ggcatgggca tcgtgatggt gttgatggga ctggcacaaa atatctggca
    38881 gtttttgatc ctgcgggcgc ttcttgggtt acttggcgga tttgtcccca acgctaatgc
    38941 tcttatcgcc acacaagtac cgcgtaataa aagcggctgg gcgctgggta cgctctccac
    39001 aggcggcgtt agtggtgcgt tgctcggccc aatggctggc ggcctgctcg ccgatagcta
    39061 cggcttacgt ccggtattct ttattaccgc cagtgtgctc atactctgct ttttcgtcac
    39121 cctgttctgc atcagagaaa aattccagcc ggtcagcaaa aaagagatgc tgcacatgcg
    39181 ggaagtggtg acatcactta aaaacccgaa actggtactc agcctgtttg tcaccacgtt
    39241 aatcatccag gtggcgacag gctcaattgc ccccattctg acgctgtatg tccgcgaact
    39301 ggcgggtaac gtcagtaacg tcgcctttat cagtggcatg atcgcctcgg tgccaggcgt
    39361 ggcggctctg ctaagcgcac cacgactcgg caaacttggc gatcgaatcg gacccgaaaa
    39421 gatcctgatt acagcgctga tcttttctgt actgctgttg atcccaatgt cttacgttca
    39481 gacgccattg caacttggga ttttacgttt tttgctcggt gccgccgatg gtgcactact
    39541 ccccgccgta cagacactgt tggtttacaa ctcgagcaac cagatcgccg ggcgtatctt
    39601 cagctataac caatcgtttc gtgatattgg caacgttacc ggaccattga tgggagcagc
    39661 gatttcagcg aactacggtt tcagagcggt atttctcgtc accgctggcg tcgtgttatt
    39721 caacgcagtc tattcatgga acagtctacg tcgtcgtcga ataccccagg tatcgaactg
    39781 atttttcgcc tttcatactt gcaaaagcgg agaatcagct atccttttcc ctgaaacctc
    39841 atcaactcaa agggagaatc gtgatgacca tgtacgcaac gcttgaagaa gccattgacg
    39901 ctgcacgcga agaatttctt gcagacaacc ccggcatcga cgccgaagat gcgaatgtgc
    39961 aacagttcaa tgcccaaaaa tacgttttgc aggacggcga catcatgtgg caagttgagt
    40021 tttttgccga cgaaggggaa gaaggtgaat gtttacctat gcttagcggt gaagccgcgc
    40081 aaagtgtttt tgatggcgac tatgatgaga tagagatacg ccaggagtgg caggaagaga
    40141 atacattaca tgaatgggac gagggggaat ttcagcttga gccaccgctg gataccgagg
    40201 aaggacgcgc agcagctgat gagtgggatg aacgttaatc actcatacgg gccatgatga
    40261 atgtcgatcg gcagcagtaa agtatcgaaa accagggaga atggcagatc aaggatcgtg
    40321 acgtaacgcc aggcggagtc acgcacgtcc cattgcacac cgggataata ttggttgcca
    40381 tgcccctgcc ccggtatagt gcgactaatg atgctgccac agccactcag caaggtcacc
    40441 attatgctca tcacaattaa acgcatttga tacctcttcc gtaaaaaata ccggcataac
    40501 gccggtattt ttattggctc gttgtttttg ccggatgcgg cgtgaacgct ttacccgacc
    40561 tacaaaatct tgcaaaatca ataaattgca ggaacgatgt aggcctgata agcgtagcgc
    40621 atcaggcaac tacgttttta ttgcgaagcc gcatccggtt tacgcaatgc cagtggattg
    40681 agctttatcc cttcgtaata actcacccat gaagaatatc tctccgggga attccagacg
    40741 cggaaatgca gacgggccat cgtcaccgga tcgcttagca gcaccaggcg acgatcgcga
    40801 ttcagcttct ctggcgtctc gttcagcgcc tgttcaacgt ggcggtcacg ggcgatttcc
    40861 agcaccttgc tggcgcggtg acgcgcggtc gccattgcgg ttgccagagc gttaaatgac
    40921 gggttaaaca ccgcgtgcat aaaaccatca tcaagggagc gttgacgatt catctcaagg
    40981 aaccgatcgg tatcaaccag cacctgcggc ggtgaatact cttccgggat caggaacagt
    41041 ttccagcgtt tggttcgcag accaacggtg gcacggctgg aaatcaccga aacaaacggt
    41101 gacaggatca acgagaagac aatcggtgcc agccagaaca gaaaacgcag atccaaccac
    41161 gccatcccaa cagcccacac taaacccaac agcagctgtg agccgtggcg tttgaatgct
    41221 tcgccccagg aagtggagtc atcatcacgc tgcggtgaat tccacaccac ttcccagcca
    41281 aggaacgcgc tgacgacgaa gactgtatgg aacaacatgc gtaccggagc cagcaacacg
    41341 gaaaacagca cttccagcag caacgataat gtaacgcgcc agaagccgcc gtattctttc
    41401 gttcctttgc accagataag caaaatgctc aatagcttcg gcaggaacaa cagcaccatg
    41461 gtcgaagcaa aaagtgcaat cgccagctca ggacgccact gcggccatac cgggaacaac
    41521 tgccgtggtt gcaggaagta ttgcggttcg gtcaacgcat gtactacctg caatgcagta
    41581 gagagcgcga ggaacataaa ccacagcgga gcggagagat aagacatcac gcccgtcagg
    41641 aacaccgcac ggtgaaccgg gtgcataccc ttcaccagga acagacggaa gttcatcagg
    41701 ttaccgtggc accagcggcg gtcacgtttt agctcatcaa gcaagttagg cggcaattct
    41761 tcataagaac ccgggagatc gtaagcaatc cagacccccc aacctgcacg gcgcatcaac
    41821 gccgcttcca cgaagtcatg tgacaggatt gaaccggcaa aagaaccttc gcccggcagc
    41881 ggagccagtg cacagtgctc gataaacggt ttcacgcgga taatcgcgtt atgcccccag
    41941 tagtgcgact cgccaagttg ccagaagtgc aaaccggctg taaacagtgg cccatacacg
    42001 cgggtcgcga actgctggca gcgcgcatac agcgtatcca tgccggacgc tttcggtgac
    42061 gactgaatga tcccggcgtt cgggttggct tccatcaggc gcaccaggcc gcacaaacaa
    42121 tcaccggtca ttaccgagtc agcatccagc accaccatgt agctgtactg gctgccccag
    42181 cgacggcaga agtcatcgat attaccgctt ttacgcttca cgcgacggcg gcggcggcga
    42241 tagaaaatct gaccttcgcc accgacttca gcgataagct ccatccaggc tttttgctct
    42301 gcgacgcaga tatccgggtt ataactgtca ctaagaatgt agacatcaaa atgtttggca
    42361 ttcccggtgg cttttactga ttcccacgtt gcacgcaagc cagcaaaaac acggttcacg
    42421 tcttcgttac agataggcat gatcaacgcc gtgcgatgct ccgggtttaa tggttcatcg
    42481 ccaactgttg acgcagatat actgtattta tcgcgaccaa taagcagttg caggaagccc
    42541 atcaacgccg tccagaatcc ggcggacacc caacagaaca gtaccgcaaa gaggatcagg
    42601 ataccggttt gcagcatata aggcagaagc tgcataaagg aaacccacac atcctgacca
    42661 accatatcca taggattaat cagcgcccac ccctgataag gaagaatggt cttcatatac
    42721 caggtcgcga caacagtttg cgcgagcgtc aggatcaaca gaatgtaacg gcggatggta
    42781 ccgacggtac gccacttttg ctcgctttcc tgctcttctt tggtcaaacg agccagatag
    42841 cgcggcgtta catcgcgtcc acgcaggcga tcccagaaac ggcctaccgg gttggtacgc
    42901 cacgggtcgg gaaacatcga ggagcgtttt acttctggca tcgcctttag ctgatcgcgc
    42961 ccttcgtcgt ctttaattaa ctgtccatca gcaagtgaat ctggccaggc ttgttccaga
    43021 cgcgccttta ccgagccttg cggggagtca tcctcccgcg cccaggtgcg gtgttcggca
    43081 tccagcgcct gatgaacggc gcggatatca gtcttcggca atgccgcttt ctcgcttgcg
    43141 gcgatgggca ttgcgtcaat gtactcagtt gtcttattca ttggcaggta actggtagct
    43201 ccaggtttca ctcaacgtct gatcggcatt caccagcgca gcacgcattt cagtggtttt
    43261 cttggcatct ttcactttca cacgcatcac cagacgccaa cctttggtaa ccgggttata
    43321 acgcaccgtg ctttcaacta tctcaccatt atcaccaatg ctggtttgcg ctgtgaccgg
    43381 ggtatcctct ggcagttttt tcatctcagc gccggtaaaa tcgaccacaa aggcgatagt
    43441 accgtcaggc tggcgaatca ggttcgactg cttcacatcc cccgttgaac gacgcgtttg
    43501 ttgcacccat gcgttatctg gcgcatgcag tttgtcttca tcacggctga aggtgatggt
    43561 gtatttaaag ttcatctctt tacccggctc cggcagctga tccggcgtcc agtaagcgac
    43621 gatgttatcg ttggtttcat cgttggttgg aatttccacc agctcaacgc tgcctttgcc
    43681 ccactcccct ttcggagtca cccatgcgct tggacgaaga tcgtaacgat catcgagatc
    43741 ttcaaagcgg gagaaatcac gaccacgctg caatagaccg aagccttgcg ggttttccat
    43801 cgagaagctg ctgaccgcta aatgtttcgg gttattcaac ggacgccaga tccattcgcc
    43861 attaccagca tggatagaca gaccgttaga gtcgtgcaac tccggacgat agttatttgc
    43921 aggcgacggt tggttcggcc caaacaggaa catactggtt aacggtgcaa cccccagttt
    43981 gccgacttta tcgcgcagat agattttcga ctgcacatcc acaaccgtgt cacgtcctgg
    44041 cataactacg aatttgtaag cacctgtcgc gcgcggcgag tcaagcaatg cataaatggt
    44101 taaacgttta tcagtcggtt ttggacgctc gatccagaac tctttgaagc gtggaaattc
    44161 ttcacccgat ggcaaggcgg tatcaattgc caggccgcgt gcagaaaggc cataaacctg
    44221 acctgcacca atcacgcgga aatagctggc cccgagcatg ctgacgattt catcgttttt
    44281 atctttgctg ttgatcgggt aaagcacttt gaaaccggca aaaccaaggt cttttaccgt
    44341 gtctttgtca tgctgaacat cgccgaaagt gaaataatcc gggctgtatt tgattcgttt
    44401 gactgcggtg gcagtcactt catttatttt gaccggggta tcgaagtaca taccctgatg
    44461 gtagaactcg agtttgaatg gggtcttcag attgttccag tacgctttgt catgattaaa
    44521 ctggatctgc tgatagtccg cgtatttcat atcgcggaaa acggagggca agttgctttt
    44581 gggcgcctca tagcctttcc cggctaagga ttgagcttgc tttgcgacat catcaatact
    44641 gaaagcccag cttgaagatg tatacagggt taacattact gcagcactca accaacgcat
    44701 tttcatcatt tgtagtttat gtttcataat tagtaagcac ttcccccttt gtgtgcttat
    44761 atcgatccga tctattttaa tggataacct gccattccga caacagaatc ccggtatcgt
    44821 tcagcacact ggctcatgga ttaagcacct gattgcaacc gttttcactt tccgtgcttc
    44881 tcctaaagac agaagcatcg acttcgtgta gggtttgcca gcaaatttat ttatcaaccg
    44941 tcttacagac gcttccattc tggttagtgg tagcgatgat gctatgcata aaaaccttga
    45001 agcctgcgcc tggcgcggtc aggtcggtgt cgctatggca acaagcctaa aggcataacc
    45061 cgacagggat aagacgaaaa atccgagatt agttaaccat atatgaatcc agtacccgcg
    45121 caacgtgaat atttcctcga ctccatccgc gcctggctga tgttgttagg gatccctttt
    45181 catatttctt taatctattc gagccataca tggcatgtga atagcgccga accgtcattg
    45241 tggctgaccc tttttaatga cttcatccac tcgttccgca tgcaggtatt tttcgttata
    45301 tccgggtact tttcctacat gcttttttta cgctatccct tgaaaaaatg gtggaaagta
    45361 cgtgtcgaac gtgtaggtat cccgatgtta acagccatcc ccctactgac attaccgcaa
    45421 tttattatgc tgcaatacgt caaagggaaa gcggaaagtt ggcctgggct atcattgtat
    45481 gacaaatata atacgttggc ctgggaatta atatcacacc tgtggttttt actggtgtta
    45541 gtagtcatga cgacgctgtg cgtatggata tttaagcgca tcagaaataa tttagaaaat
    45601 tctgataaaa cgaataaaaa attctcgatg gtaaaactat cggtgatttt tttgtgcctc
    45661 ggcatcggtt atgcggtaat aagaagaacg atttttattg tgtatccgcc cattctgagt
    45721 aatggcatgt tcaattttat tgtcatgcaa acgctatttt atttgccgtt ctttatcctc
    45781 ggcgcactgg ctttcatttt ccctcatctt aaagccttgt ttaccacgcc gtctcgtggc
    45841 tgtacccttg cagcagcatt ggcatttgtc gcttatttac tcaaccagcg ctatggcagt
    45901 ggcgatgcct ggatgtacga aaccgagtcg gtgatcacca tggtcctcgg tctgtggatg
    45961 gtgaatgtgg tcttctcctt tggccaccgt ttgcttaact tccagtcagc gcgggtgact
    46021 tattttgtta acgcatcgct gtttatctat ctggttcacc acccgttaac gctgtttttc
    46081 ggcgcataca ttacaccaca catcacctcc aactggcttg gttttctctg tggcctgata
    46141 ttcgtagtag ggattgcgat aattctgtat gaaattcatt tacgcatccc attattgaag
    46201 tttttgttct ctggtaaacc ggttgttaag cgtgagaacg ataaagcacc agcccgttaa
    46261 gccacgctta taataaccat tccacgggca atatcgacgc cagtctgacc ataacccgct
    46321 tccagaaact ggtggcgggt tcttttttga gaataatctc tttctttgca tgacgatcaa
    46381 cccagttgat ccgtccccac ctgtccagac ggagctgcca ggccgcatca tactggctct
    46441 gaataaagcg tttatcaatt aactgtgcca acgtttcgct ctctatcacg aagcccattt
    46501 cagtattgag caatgtcgaa cgtggatcga aattgaaaga gccgataaac accgttttac
    46561 catcgatgct aaaggtttta gcatgcaagc tggcaccgga attaccggtt ataccgcgat
    46621 cgtgtaacgt actactttgt tcacgcgtcg gcttgagttc atataattcc acgccatagc
    46681 ggagcaattt tttgcgccag cgcgcgtatc cggcatggac gacagcaaca tcgttagcgg
    46741 caagagaatt ggttaggatc gcaatcttta ccccttttct caccatccgt aagagttgcg
    46801 ccacacctgc gcgtgtcggt acaaaatagg aagagataat atcgatgcgt tcactgggtg
    46861 agcccatgat atcgaacagg cgctgcggta acagtgaatg ccgttttgcc ttgccttccc
    46921 ctttcgccgg atcatcactt aataaacgtg ttttcgccca gataagcggc aacgttccat
    46981 caaccagatg atttataaac gggctggatt ccattttgcg taaataacga tgcgtcatgg
    47041 catcgttgtg ccaggatgct ggtaactcga tgcgatccgc catttcacct tccgggacat
    47101 ccagcacctg ctgtaggggt gaaaccgatt tgcaatacca gtagcgggcg aaatcatcgg
    47161 caacgtcctc tacaactggt cctattgcca tgacatctaa atccgaaaaa agtggctctt
    47221 cccctgcacc aaaataggca tcaccaatat ttcgcccccc taccagggta accacgccat
    47281 cgacagtgaa acttttattg tgcatacggc gattgagacg ggaaaagtcg gtgatataac
    47341 caagcggacg cagcaagcga aacgagaaag gattaaaaag ccggacttca atgcggggat
    47401 gactgtcaag caagcgtaaa atgtcgtcaa gtccgggcgt attgttgtca tccagcaaca
    47461 aacggacgcg aacgccacgc tttgctgcgg ctaacagggc ggaaaagagt aaccgacccg
    47521 acatatcgtc ctgccagatg taatactgaa catccagcgt atgctctgcc atttcggcca
    47581 gacggtaccg ggcggcaaac gcatccagac ttttctccag aggaaaaagg ccacactgac
    47641 cgggatgttg cgaacacagt ggcagcaccg cgctcgccag ccggggcaaa tcattcatct
    47701 ccttgttgag taaggagtct ttcgtagagg tgggcgtttt cttcatcata acagacaaag
    47761 tatacctgtt cgggtaaagc gtgacgggta ataaattctg aaacggtttt tactgcgatt
    47821 tcagccgctg ccgcacgagg gtaaccataa accccagtac tgattgcagg aaaagccact
    47881 gacgtatagc tgtttgccgc caccagtcgt aggctattga gataggcatc ctgcaaaagc
    47941 tggtcttcgt tttgttcacc accacgccag actggcccga cggtgtgcac tacggcttta
    48001 gcaggaagat cgcctgcaag cgtaataacg gcatgccccg tagggcaatc gccctgctgc
    48061 tgcctgactt ttaaacaggc atccaacagg gccggacccg ctgcgcgatg aatggcccca
    48121 tcgacgccgc cgcctcccat taatgacgga ttagccgcat tcacaatcac atcaacggcc
    48181 agtttggtaa tatcaccctg cacaacatga atacgcgttt tcataacgcc tccttacacc
    48241 ttcttgtttc ttaagtgtac agcaggcttt ttggttaaat cgcatttttc tgctcagcaa
    48301 cgtgagttgt tcagaaactt agttatattc gatgttcatt acagccagct ttttctgttt
    48361 atcaagatac tgcactttta ctgaggctat ttgcgcgttt ttgacattgc cgctcattaa
    48421 cgctttgctg gagtaggttt tgcccggaac agaaccattc tgtgggcagt tcatttctac
    48481 gggtgatgac tgggtgctga cgtcacatgc cggttccaca atttggccat aaaaatggat
    48541 cacgccccct tgggcggtgc tttctgcaat ggcaataaaa ggaaaaaaca aactgccgag
    48601 cataagagag tttaaaaaag gtctgaacat ttattctatt tcctcaatga ttagtcatcc
    48661 ttgagggttg tgttatccat actttcaggt tggctgattc gcagcagacc attctctcca
    48721 gattcatctt atgctcgata tttcaataga ttaagacttt tctgaagagg gcggccattg
    48781 ttgtgataaa tgaagtgact gtccatcaga aacagtaaca actattttca cccgatcgtc
    48841 cggggaaata tttaaactca acttcgtcaa agcaatgggt tgattagcag gcaatgaaag
    48901 ggtcttttct tgcttcgtct gactttgccc tgaactgcct tcgcgcaggg acaaaatttg
    48961 tactctgcac agacaagatt gagtaagagt gacttcagga ataatggtgt acatatcccc
    49021 ttgctgggtc gtattaaagg ttatctgact ggaaagtgcc gcaaggagta ataacgcatt
    49081 catattcttc tcccgaaaaa aaacagggct tgcgccctgt ttctttaata cagatgatgt
    49141 attagtactg atgagcggtc gcgttgttac caaagccaac ctgagtcacg ttgacggagg
    49201 agttagatgc agtctgatca actgcagcac cgttgccacc accgaactgt ttaacagtca
    49261 tttcagaatt tttgccgttc cactgatcaa gagtggcgct gttaccgaag ccacgttggg
    49321 tcagatcgat tgagctgtca tctgagccct gaccaacatc tgcaccatta ccaccgccat
    49381 gttgggtaat agtcaagtca gagttacggg catcagcttg cagagcaagt gcagagttac
    49441 cgccaccgta ctggtaaatg ttcagctctg aatttgggcc gctattatta ccgccaccac
    49501 cgtggttgcc gccaccgccg ccgtactgag gaacaacacc tgccagagcg ctaccggaga
    49561 atacgattgc tgcaattgct gctactttta aaagtttcat gtaaaacccc catcggattg
    49621 atttaaaagt cgaatggaaa ttaacgttgt gtcacgcgaa tagccatttg cgactgtctc
    49681 tgcactacaa ttgccgtttt ttgagtacca tactgtgtaa tatttgcttt attacccgaa
    49741 cctttctgga taatcatcgc agtattacca taagcacctt gcgaaatact ggcatcgttg
    49801 gcactgcccg cctgatcaat atatgcaagg ttataatctc ctgtctggtc aatctttgcc
    49861 cggttgctac taccttcttg cgcaacaacc gccaaaagtt ttgagcctcc ctgccgtaac
    49921 tgagcactat tattagtccc agcttgacca attatggctg cctgattaaa tgaagactta
    49981 ctcaattcat ttaccgcgaa gttatattct gaattagcta aatcataacc tgctgcggct
    50041 gcaatcccag gcgcacccag tattgttaac atcataaata acaatttgtt tttcatgttg
    50101 tcaccctgga cctggtcgta catttaagaa attaaatcat ttcaacttgg ttgttaacgc
    50161 aacctgtatt ttgttaacgc tgcgttacga tggaaagtat gtctgcggaa atatttttaa
    50221 taactcaccc gcgcgttgta tttttctttt tttgttcata ccaaagtatt aaaactcacg
    50281 taaatacgct gttatctacg caaaaaatat tttgttttct tttaaatctc cgttttccgc
    50341 tatcaaaaat caccagacaa tcattcttct tgcccgtcgc tgattgctgc gatatgtctt
    50401 gcgcacaagc cgtgacaaac ctcgtctttt tgcagtaaaa aattgtccac ggaggtgtgg
    50461 agaaaaaaca agaacgtttt acatgacgaa aggactacac cgaaatattt tttat
//
LOCUS       DAPHCV010000020        49165 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.020, whole genome shotgun sequence.
ACCESSION   DAPHCV010000020 DAPHCV010000000
VERSION     DAPHCV010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.020"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            225..1793
                     /gene="cydA"
                     /locus_tag="RH033_001167"
     CDS             225..1793
                     /gene="cydA"
                     /locus_tag="RH033_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HDV2990389.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            1809..2948
                     /gene="cydB"
                     /locus_tag="RH033_001168"
     CDS             1809..2948
                     /gene="cydB"
                     /locus_tag="RH033_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDV2990390.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            2963..3076
                     /gene="cydX"
                     /locus_tag="RH033_001169"
     CDS             2963..3076
                     /gene="cydX"
                     /locus_tag="RH033_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HDV2990391.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            3076..3369
                     /gene="ybgE"
                     /locus_tag="RH033_001170"
     CDS             3076..3369
                     /gene="ybgE"
                     /locus_tag="RH033_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HDV2990392.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            3519..3923
                     /gene="ybgC"
                     /locus_tag="RH033_001171"
     CDS             3519..3923
                     /gene="ybgC"
                     /locus_tag="RH033_001171"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDV2990393.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            3920..4612
                     /gene="tolQ"
                     /locus_tag="RH033_001172"
     CDS             3920..4612
                     /gene="tolQ"
                     /locus_tag="RH033_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HDV2990394.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLSGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            4616..5044
                     /gene="tolR"
                     /locus_tag="RH033_001173"
     CDS             4616..5044
                     /gene="tolR"
                     /locus_tag="RH033_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HDV2990395.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            5109..6398
                     /gene="tolA"
                     /locus_tag="RH033_001174"
     CDS             5109..6398
                     /gene="tolA"
                     /locus_tag="RH033_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HDV2990396.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAKAEAD
                     AKAAEEAAKKAAADAKKKAEAEAAKAAVEAQKKAEAAAAALKKKAEAAEAAAAEARKK
                     AATEAAEKAKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEK
                     AAADKKAAAAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGN
                     TKNNGASGADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGD
                     PALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            6531..7823
                     /gene="tolB"
                     /locus_tag="RH033_001175"
     CDS             6531..7823
                     /gene="tolB"
                     /locus_tag="RH033_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HDV2990397.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            7858..8379
                     /gene="pal"
                     /locus_tag="RH033_001176"
     CDS             7858..8379
                     /gene="pal"
                     /locus_tag="RH033_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HDV2990398.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYAKNRRAVLVY"
     gene            8389..9180
                     /gene="cpoB"
                     /locus_tag="RH033_001177"
     CDS             8389..9180
                     /gene="cpoB"
                     /locus_tag="RH033_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HDV2990399.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGVAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            9345..9420
                     /locus_tag="RH033_001178"
     tRNA            9345..9420
                     /locus_tag="RH033_001178"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9378..9380,aa:Lys,seq:ttt)
     gene            9556..9631
                     /locus_tag="RH033_001179"
     tRNA            9556..9631
                     /locus_tag="RH033_001179"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9589..9591,aa:Val,seq:tac)
     gene            9634..9709
                     /locus_tag="RH033_001180"
     tRNA            9634..9709
                     /locus_tag="RH033_001180"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9667..9669,aa:Lys,seq:ttt)
     gene            9761..9836
                     /locus_tag="RH033_001181"
     tRNA            9761..9836
                     /locus_tag="RH033_001181"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9794..9796,aa:Val,seq:tac)
     gene            9840..9915
                     /locus_tag="RH033_001182"
     tRNA            9840..9915
                     /locus_tag="RH033_001182"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9873..9875,aa:Lys,seq:ttt)
     gene            9964..10039
                     /locus_tag="RH033_001183"
     tRNA            9964..10039
                     /locus_tag="RH033_001183"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9997..9999,aa:Lys,seq:ttt)
     gene            10073..10148
                     /locus_tag="RH033_001184"
     tRNA            10073..10148
                     /locus_tag="RH033_001184"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10106..10108,aa:Lys,seq:ttt)
     gene            10426..11469
                     /gene="nadA"
                     /locus_tag="RH033_001185"
     CDS             10426..11469
                     /gene="nadA"
                     /locus_tag="RH033_001185"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HDV2990400.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            11507..12226
                     /gene="pnuC"
                     /locus_tag="RH033_001186"
     CDS             11507..12226
                     /gene="pnuC"
                     /locus_tag="RH033_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HDV2990401.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(12223..13164)
                     /gene="zitB"
                     /locus_tag="RH033_001187"
     CDS             complement(12223..13164)
                     /gene="zitB"
                     /locus_tag="RH033_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HDV2990402.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(13278..13658)
                     /locus_tag="RH033_001188"
     CDS             complement(13278..13658)
                     /locus_tag="RH033_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HDV2990403.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQSNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            13974..15026
                     /gene="aroG"
                     /locus_tag="RH033_001189"
     CDS             13974..15026
                     /gene="aroG"
                     /locus_tag="RH033_001189"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HDV2990404.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLTLREELQDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWDDT
                     DALLRQLANAVKARRG"
     gene            complement(15174..15926)
                     /gene="gpmA"
                     /locus_tag="RH033_001190"
     CDS             complement(15174..15926)
                     /gene="gpmA"
                     /locus_tag="RH033_001190"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDV2990405.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(16129..17169)
                     /gene="galM"
                     /locus_tag="RH033_001191"
     CDS             complement(16129..17169)
                     /gene="galM"
                     /locus_tag="RH033_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HDV2990406.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPF
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNYLGGTPSRGTEPYTDWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAQ"
     gene            complement(17163..18311)
                     /gene="galK"
                     /locus_tag="RH033_001192"
     CDS             complement(17163..18311)
                     /gene="galK"
                     /locus_tag="RH033_001192"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDV2990407.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDATIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(18315..19361)
                     /gene="galT"
                     /locus_tag="RH033_001193"
     CDS             complement(18315..19361)
                     /gene="galT"
                     /locus_tag="RH033_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDV2990408.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESHDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(19371..20387)
                     /gene="galE"
                     /locus_tag="RH033_001194"
     CDS             complement(19371..20387)
                     /gene="galE"
                     /locus_tag="RH033_001194"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDV2990409.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(20648..22120)
                     /gene="modF"
                     /locus_tag="RH033_001195"
     CDS             complement(20648..22120)
                     /gene="modF"
                     /locus_tag="RH033_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HDV2990410.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARAQAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAELLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGDFYRYALTKIN"
     gene            complement(22188..22976)
                     /gene="modE"
                     /locus_tag="RH033_001196"
     CDS             complement(22188..22976)
                     /gene="modE"
                     /locus_tag="RH033_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HDV2990411.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            23105..23254
                     /gene="acrZ"
                     /locus_tag="RH033_001197"
     CDS             23105..23254
                     /gene="acrZ"
                     /locus_tag="RH033_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HDV2990412.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            23420..24193
                     /gene="modA"
                     /locus_tag="RH033_001198"
     CDS             23420..24193
                     /gene="modA"
                     /locus_tag="RH033_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV2990413.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            24193..24882
                     /gene="modB"
                     /locus_tag="RH033_001199"
     CDS             24193..24882
                     /gene="modB"
                     /locus_tag="RH033_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDV2990414.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDIKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            24885..25943
                     /gene="modC"
                     /locus_tag="RH033_001200"
     CDS             24885..25943
                     /gene="modC"
                     /locus_tag="RH033_001200"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HDV2990415.1"
                     /translation="MLELNFSQTLGNHCLTIDETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLSPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVNQFDKLVALLGIEPLLERLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(25944..26762)
                     /gene="ybhA"
                     /locus_tag="RH033_001201"
     CDS             complement(25944..26762)
                     /gene="ybhA"
                     /locus_tag="RH033_001201"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HDV2990416.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQNFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            26917..27912
                     /gene="pgl"
                     /locus_tag="RH033_001202"
     CDS             26917..27912
                     /gene="pgl"
                     /locus_tag="RH033_001202"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HDV2990417.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(27953..28906)
                     /locus_tag="RH033_001203"
     CDS             complement(27953..28906)
                     /locus_tag="RH033_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2990418.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            29090..30142
                     /locus_tag="RH033_001204"
     CDS             29090..30142
                     /locus_tag="RH033_001204"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HDV2990419.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLASIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISCSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            30218..31651
                     /locus_tag="RH033_001205"
     CDS             30218..31651
                     /locus_tag="RH033_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDV2990420.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSGGEFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGSTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            31834..34095
                     /locus_tag="RH033_001206"
     CDS             31834..34095
                     /locus_tag="RH033_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HDV2990421.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEAPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(34236..35519)
                     /locus_tag="RH033_001207"
     CDS             complement(34236..35519)
                     /locus_tag="RH033_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HDV2990422.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEVQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(35654..36724)
                     /locus_tag="RH033_001208"
     CDS             complement(35654..36724)
                     /locus_tag="RH033_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDV2990423.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKKILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            complement(36702..36920)
                     /locus_tag="RH033_001209"
     CDS             complement(36702..36920)
                     /locus_tag="RH033_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDV2990424.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            complement(36960..37127)
                     /locus_tag="RH033_001210"
     CDS             complement(36960..37127)
                     /locus_tag="RH033_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2990425.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENISDCYDHWMLWAQIAHADVTNI
                     RIEELKEHQAA"
     gene            complement(37364..38062)
                     /locus_tag="RH033_001211"
     CDS             complement(37364..38062)
                     /locus_tag="RH033_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HDV2990426.1"
                     /translation="MKNILLASLLVASPGAFAASFDCQKASTAIEHKICDNERLSKLD
                     EQLSSAYSSALKGNPENADTLKMVQRQWVNMRGKLTDNKALELAYLIQINGLKGLGSS
                     VSVTAANDIPTSAQKHSKEQEDTSKAEAKSVKNGNKLTLESFRAKYVEVDGEYYSTTS
                     IPRGSSFLFTCASRIADDQVNIWKKQAAKEGKIDLFFEVENHLHTAMLNANFQKLNSD
                     PAKRGICNLINAVP"
     gene            complement(38193..38741)
                     /locus_tag="RH033_001212"
     CDS             complement(38193..38741)
                     /locus_tag="RH033_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HDV2990427.1"
                     /translation="MSEINSQALREAAVAIETVATPQKLLAFRMKVTPQVVLALLDER
                     DALNERLAELEADLAGLAEDHQKATESIKQADAAVKLAHEKFSALAAENELARKAVQE
                     FCDVVGDSTEVICEEIGRDGVLVILEAMKATGNMPATDAFLAEVRAQGVEMMREHPSI
                     KLCSLTHICDELAAQLRKGGNQ"
     gene            complement(38738..38959)
                     /locus_tag="RH033_001213"
     CDS             complement(38738..38959)
                     /locus_tag="RH033_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HDV2990428.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            complement(39058..39273)
                     /locus_tag="RH033_001214"
     CDS             complement(39058..39273)
                     /locus_tag="RH033_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDV2990429.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            complement(39350..39541)
                     /locus_tag="RH033_001215"
     CDS             complement(39350..39541)
                     /locus_tag="RH033_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HDV2990430.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLAASL
                     SQKLEMMVAKAEADERDQV"
     gene            complement(39514..39696)
                     /locus_tag="RH033_001216"
     CDS             complement(39514..39696)
                     /locus_tag="RH033_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HDV2990431.1"
                     /translation="MTHPHDNIRVGAITFVYSITKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLPLS"
     gene            complement(39693..40373)
                     /locus_tag="RH033_001217"
     CDS             complement(39693..40373)
                     /locus_tag="RH033_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HDV2990432.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDENYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            complement(40370..41155)
                     /gene="bet"
                     /locus_tag="RH033_001218"
     CDS             complement(40370..41155)
                     /gene="bet"
                     /locus_tag="RH033_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HDV2990433.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTMLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQVEAMKALGFLKQKASEQKVAA"
     gene            complement(41161..41457)
                     /locus_tag="RH033_001219"
     CDS             complement(41161..41457)
                     /locus_tag="RH033_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HDV2990434.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            complement(41532..41675)
                     /gene="kil"
                     /locus_tag="RH033_001220"
     CDS             complement(41532..41675)
                     /gene="kil"
                     /locus_tag="RH033_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HDV2990435.1"
                     /translation="MDKKLMAIQTKFTIATFIGDEKMFREAVDAYKKWILILKLRSSK
                     SIH"
     gene            complement(41668..41808)
                     /locus_tag="RH033_001221"
     CDS             complement(41668..41808)
                     /locus_tag="RH033_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HDV2990436.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NG"
     gene            complement(41881..42249)
                     /locus_tag="RH033_001222"
     CDS             complement(41881..42249)
                     /locus_tag="RH033_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HDV2990437.1"
                     /translation="MSNIKKYIIDYDWKASIEIEIDHDVMTEEKLHQINNFWSDSEYR
                     LNKHGSVLNAVLIMLAQHALLIAISSDLNAYGVVCEFDWNDGNGQEGWPPMDGSEGIR
                     ITDIDTSGIFDSDDMAIKAA"
     gene            42506..43000
                     /locus_tag="RH033_001223"
     CDS             42506..43000
                     /locus_tag="RH033_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2990438.1"
                     /translation="MSDEKGIISRITDAVSGAGGALMSAVGAVKEIQKMQIDYSVKEK
                     TYELVDKLMDAQQQQMSLNELLMISKDKIIELEEKINRASKWEEEKKNYEMYTPTVAT
                     VVYRLKKSANTGQPMHYLCAQCYESSVKSILQYEGFAPPSNHRMRCHRCNASYLFPKS
                     AFSK"
     gene            complement(42993..43316)
                     /locus_tag="RH033_001224"
     CDS             complement(42993..43316)
                     /locus_tag="RH033_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HDV2990439.1"
                     /translation="MDAQTRRRERRAEKQAQWKAANPLLVGVSAKPVNRPILSLNRKP
                     KSRVESALNPIDLTVLAEYHEQIESNLQRIERKNQRTWYSKPGERGITCRGRQKIKGK
                     SISLI"
     gene            complement(43798..44655)
                     /locus_tag="RH033_001225"
     CDS             complement(43798..44655)
                     /locus_tag="RH033_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDV2990440.1"
                     /translation="MKKAAILIDAGFFMQRVHATHRKHFAEHELTAQCIMKVIWSMVL
                     SHLNGKRQSQERREPLELYRIYFYDCPPLDIQTRLPLPEPGNKTPGRKNFKLEKSYIL
                     RTELHEELRKTRKTALRLGNLVDNKRWQLTTFSLDALMKGTKKWDELTNDDFYYDIKQ
                     KQVDIKLGMDITTLAYEKLVDVIVLVAGDSDFVPAAKHARIKGIDFILDPLRQNVTPS
                     LSEHIDGVQSYSLISGLADALHVEPDPAPDWWEDRKKGKPRGKNNSGKRRYGNTQAES
                     AKKHQRNKR"
     gene            complement(44783..45496)
                     /locus_tag="RH033_001226"
     CDS             complement(44783..45496)
                     /locus_tag="RH033_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HDV2990441.1"
                     /translation="MSAKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMG
                     QSGVGALFNGINALNAYNAALLAKILNVSVEEFSPSIAREIYEMYEAVSMQPSLRSEY
                     EYPVFSHVQAGMFSPELRTFTKGDAEKWVSTTKKASGSAFWLEVEGNSMTAPTGSKPS
                     FPDGMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNE
                     SCSVVGKVIASQWPEETFG"
     gene            45597..45797
                     /locus_tag="RH033_001227"
     CDS             45597..45797
                     /locus_tag="RH033_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDV2990442.1"
                     /translation="MEQRITLKDYAMRFGQTKTAKDLGVYQSAINKAIHAGRKIFLTI
                     NADGSVYAEEVKPFPSNKKTTA"
     gene            45916..46209
                     /locus_tag="RH033_001228"
     CDS             45916..46209
                     /locus_tag="RH033_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HDV2990443.1"
                     /translation="MVRANKRNEALRIESALLNKIAMLGTEKTAEAVGVDKSQISRWK
                     RDWIPKFSMLLAVLEWGVVDDDMARLARQVAAILTNKKRPAATERSEQIQMEF"
     gene            46242..47141
                     /locus_tag="RH033_001229"
     CDS             46242..47141
                     /locus_tag="RH033_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDV2990444.1"
                     /translation="MTNTAKILNFGRGNFAGQERNVADLDDGYARLSNMLLEAYSGAD
                     LTKRQFKVLLAILRKTYGWNKPMDRITDSQLSEITKLPVKRCNEAKLELVRMNIIKQQ
                     GGMFGPNKNISEWCIPQNEGQSPKTRDKTSLKLGDCYPSKQGDTKDTITKEKRKDYSS
                     ENSGESSDQPENDLSVVKPDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFA
                     GWANDIRLMRERDGRNHRDMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQ
                     AGVTASKPKLDLTNTDWIYGVDL"
     gene            47138..47839
                     /locus_tag="RH033_001230"
     CDS             47138..47839
                     /locus_tag="RH033_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein P"
                     /protein_id="HDV2990445.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELVHMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            47836..48126
                     /locus_tag="RH033_001231"
     CDS             47836..48126
                     /locus_tag="RH033_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HDV2990446.1"
                     /translation="MTGKEAIIHYLGTHNSFCAPDVAALTGATVTSINQAAAKMARAG
                     LLVIEGKVWRTVYYRFATKEEREGKMSTNLIFKECRQSAAMKRVLAVYGVKR"
     gene            48200..48640
                     /locus_tag="RH033_001232"
     CDS             48200..48640
                     /locus_tag="RH033_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HDV2990447.1"
                     /translation="MKKLTFEIRSPAHQQNAIHAVQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGQSTS
                     RMRVGEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDRAA"
     gene            48637..49164
                     /locus_tag="RH033_001233"
     CDS             48637..49164
                     /locus_tag="RH033_001233"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HDV2990448.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEADDALNSEWVSHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTTVS
                     KAALMAIGQGVRRAA"
BASE COUNT        11997 a        11908 c        12569 g        12691 t
ORIGIN      
        1 caataaaaac ctgtttattg taaggatttt gcggcgtaat ataattacgg gatcaatttg
       61 gtttattcat taacacattt gtaacttttt tatcattgtc ttttcctctt ttgatgaaat
      121 ggcaacgata gaggcgagca aataattttg tattggggca tgcgtgtgac cctttctaac
      181 ggggttcact ctcggagtct tcatgcgatg agcaaggagt catgatgtta gatatagtcg
      241 aactgtcgcg cttacagttt gccttgaccg cgatgtacca cttccttttt gtgccactga
      301 cgctcggtat ggcgttcctg ctggccatta tggaaacggt ctacgtcctt tccggcaaac
      361 agatttataa agatatgacc aagttctggg gcaagttgtt tggtatcaac tttgctctgg
      421 gtgtggctac cggtctgacc atggagttcc agttcgggac taactggtct tactattccc
      481 actatgtagg ggatatcttc ggtgcgccgc tggcaatcga aggtctgatg gccttcttcc
      541 tcgaatccac ctttgtaggt ctgttcttct tcggttggga tcgtctgggt aaagttcagc
      601 atatgtgtgt cacctggctg gtggcgctcg gttcaaacct gtccgcactg tggattctgg
      661 ttgcaaacgg ctggatgcaa aacccaatcg cgtccgattt caactttgaa actatgcgta
      721 tggagatggt gagcttctcc gagctggtgc ttaacccggt tgctcaggtg aaattcgttc
      781 acaccgtagc ttctggttat gtgactggcg caatgttcat cctcggtatc agcgcatggt
      841 atatgctgaa aggtcgtgac ttcgccttcg ctaaacgctc ctttgctatc gctgccagct
      901 tcggtatggc tgccgttctg tctgttattg ttctgggtga tgaatccggt tacgaaatgg
      961 gcgacgtgca gaaaaccaaa ctggctgcta tcgaagccga gtgggaaacg caacctgcac
     1021 ctgctgcctt tactctgttc ggcattcctg atcaggaaga ggagacgaac aaatttgcga
     1081 tccagatccc ttacgcgctg ggcatcattg caacgcgttc cgtggatact ccagttatcg
     1141 gcctgaaaga actgatggtg cagcatgaag aacgcattcg taacgggatg aaggcgtact
     1201 ctctgctcga acaactgcgt tctggttcta ctgaccaggc cgttcgtgac cagttcaata
     1261 gcatgaagaa agatctcggt tacggtctgc tgctgaaacg ctatacgcca aacgtggctg
     1321 atgcgactga agctcagatt caacaggcaa ccaaagactc cattccgcgt gtagcgccgc
     1381 tgtacttcgc gttccgtatc atggtggcgt gtggcttcct gctgctggca atcattgcgc
     1441 tctctttctg gagtgtcatc cgcaaccgca ttggcgagaa aaaatggctg ctgcgcgccg
     1501 cgctgtacgg tattccgctg ccgtggattg ctgtagaagc gggctggttc gtggctgaat
     1561 atggccgcca accgtgggct atcggtgaag tgctgccgac agctgtggcg aattcgtcac
     1621 tgaccgcagg cgatctcatc ttctcaatgg tgctgatttg cggcctgtat accctgttcc
     1681 tggtggcaga attgttctta atgttcaagt ttgcacgcct cggcccaagc agcctgaaaa
     1741 ccggtcgcta tcactttgag cagtcttcca cgactactca gccggcacgc taagacagga
     1801 gtcgtcaaat gatcgattat gaagtattgc gttttatctg gtggctgctg gttggcgttc
     1861 tgctgattgg ttttgcagtc actgacggtt tcgacatggg ggtgggcatg ctcacccgtt
     1921 tcctcggtcg taacgacacc gagcgtcgaa ttatgattaa ctccatcgca ccacactggg
     1981 acggtaacca ggtttggctg atcaccgcag gcggcgcact gtttgctgcc tggccgatgg
     2041 tctatgccgc tgcgttctcc ggcttctatg tggcgatgat cctcgtgctg gcgtctttgt
     2101 tcttccgtcc ggtcggtttt gattaccgct ccaagattga agaaacccgc tggcgtaaca
     2161 tgtgggactg gggcatcttc attggtagct tcgttccgcc gctggtgatt ggtgtagcgt
     2221 tcggtaacct gttgcagggc gtaccattca acgttgatga atatctgcgt ctgtactaca
     2281 ccggtaactt cttccagctg cttaacccgt tcggcctgct ggcaggtgtg gtgagcgtag
     2341 ggatgatcat tactcagggc gcaacctatc tgcaaatgcg taccgtgggc gaactgcacc
     2401 tgcgtacccg tgcaacggct caggtggcgg cactggtgac gctggtctgc ttcgcactgg
     2461 ctggcgtatg ggtgatgtac ggtatcgatg gttatgtcgt gaaatcgaca atggaccatt
     2521 acgcagcctc taacccgctg aataaagaag tggttcgtga agctggcgca tggctggtta
     2581 acttcaacaa cacgccaatt ctgtgggcta ttccggcact gggtgtggtt ctgccgctgc
     2641 tgaccatcct gactgcacgt atggataaag ccgcgtgggc gtttgtgttc tcctccctga
     2701 cgctggcctg catcatcctg actgccggta tcgcaatgtt cccgtttgtg atgccgtcca
     2761 gcaccatgat gaacgcaagt ctgacaatgt gggatgcaac ttccagccag ctgacgctta
     2821 acgtcatgac ctgggttgcg gtggttctgg taccgatcat tctgctctac accgcctggt
     2881 gttactggaa aatgttcggt cgtatcacca aagaagatat tgaacgtaac acccactctc
     2941 tgtactaagt aaggagctaa aaatgtggta tttcgcatgg attctgggaa cgcttcttgc
     3001 ctgttcgttt ggggtaatca ccgcgctggc gcttgagcac gtcgaatcag gcaaagccgg
     3061 tcaagaagac atctgatgag taagattatc gcaaccttgt atgcggtaat ggacaagcgc
     3121 cccctgcggg cgctttcctt cgtgatggca cttctgttag caggatgtat gttttgggac
     3181 ccatcacgtt tcgccgcgaa gaccagtgaa ctggaaatct ggcatggttt actgctgatg
     3241 tgggccgtct gtgctggtgt gattcacggc gtgggctttc gtccgcagaa ggttctctgg
     3301 caagggattt tttgcccatt gcttgccgat attgttctca ttgtcgggct gattttcttc
     3361 ttcttttaaa tcagaattct ctttaaaaat ttatgggccg agtcaggccc ataaattttt
     3421 acgctccctt aacttgccct cattcccaaa cctcaatcgc gcgcgtatag tagcagcgtt
     3481 taaaagctct aacttttgtt gcattaccgg gatgtaaagt gaatacaacg ctgtttcgat
     3541 ggccggttcg cgtctactat gaagataccg atgccggtgg tgtggtgtac cacgccagtt
     3601 acgtcgcttt ttatgaaaga gcacgcacag agatgctgcg tcatcatcat ttcagtcaac
     3661 aggcgctgat ggctgaacgc gttgcctttg tggtacgtaa aatgacggtg gaatattacg
     3721 cacctgcgcg gctcgacgat atgctcgaaa tacagactga aataacatca atgcgtggca
     3781 cctctttggt tttcacgcaa cgtattgtca acgccgagaa tactttgttg aatgaagcag
     3841 aggttctggt tgtttgcgtt gacccactca aaatgaagcc tcgtgcgctt cccaagtcta
     3901 ttgtcgcgga gtttaagcag tgactgacat gaatatcctt gatttgttcc tgaaggctag
     3961 ccttctggtt aaacttatca tgttgatttt gattggtttt tcaatcgcat cctgggccat
     4021 tattatccag cggacccgta ttcttaacgc agcggcgcgc gaagccgaag cgtttgaaga
     4081 taaattctgg tctggaatcg aactctctcg cctctatcaa gagagccagg ggaaacggga
     4141 taatctgagc ggttcggaac aaatctttta cagcggcttc aaagagtttg tgcgcctgca
     4201 tcgtgccaat agccatgcgc cggaagccgt agtggaaggg gcgtcgcgtg cgatgcgtat
     4261 ttccatgaat cgtgaacttg aaaatctgga aacgcacatt ccgttcctcg gtacggttgg
     4321 ctccatcagc ccgtatattg gtctgtttgg tacggtctgg gggatcatgc acgcctttat
     4381 cgccctcggg gcggtaaaac aagcaacact gcaaatggtt gcgcccggta tcgcagaagc
     4441 gttgattgcg acggcaattg gtctgttcgc cgcaattccg gcggtaatgg cttataaccg
     4501 tctcaaccag cgcgtaaaca aactggaact gaattacgac aactttatgg aagagtttac
     4561 cgcgattctg caccgccagg cgtttaccgt tagcgagagc aacaaggggt aagccatggc
     4621 cagagcgcgt ggacgaggtc gtcgcgatct caagtccgaa atcaacattg taccgttgct
     4681 ggacgtactg ctggtgctgt tgctgatctt tatggcgaca gcgcccatca tcacccagag
     4741 cgtggaggtc gatctgccag acgctactga atcacaggcg gtgagcagta acgataatcc
     4801 gccagtgatt gttgaagtgt ctggtattgg tcagtacacc gtggtggttg agaaagatcg
     4861 tctggagcgt ttaccaccag agcaggtggt ggcggaagtg tccagccgtt tcaaggccaa
     4921 cccgaaaacg gtctttctga tcggtggcgc aaaagatgtg ccttacgatg aaataattaa
     4981 agcactgaac ttgttacata gtgcgggtgt gaaatcggtt ggtttaatga cgcagcctat
     5041 ctaaacatct gcgtttccct tgcttgaaag agagcgggta acaggcgaac agtttttgga
     5101 aaccgagagt gtcaaaggca accgaacaaa acgacaagct caagcgggcg ataattattt
     5161 cagcagtgct gcatgtcatc ttatttgcgg cgctgatctg gagttcgttc gatgagaata
     5221 tagaagcttc agccggaggc ggcggtggtt cgtccatcga cgctgtcatg gttgattcag
     5281 gtgcggtagt tgagcagtac aaacgtatgc aaagccagga atcaagcgcg aagcgttctg
     5341 atgagcagcg caagatgaag gagcagcagg ctgctgaaga actgcgtgag aaacaagcgg
     5401 ctgaacagga acgcctgaag caacttgaga aagagcggtt agcggctcag gagcagaaaa
     5461 agcaggctga agaagccgca aaacaggccg agttaaagca gaagcaagcg gaagaggcgg
     5521 cagcgaaagc ggcggcagat gctaaagcga aggccgaagc ggatgctaaa gcgaaggccg
     5581 aagcggatgc taaagctgcg gaagaagcag cgaagaaagc ggcggcagac gcgaagaaaa
     5641 aagcagaagc agaagccgcc aaagccgcag tcgaagcgca gaaaaaagcc gaggctgctg
     5701 ctgcggcgct gaagaagaaa gctgaagcgg cagaagcagc tgcagctgaa gcaagaaaga
     5761 aagcggcaac cgaagctgct gaaaaagcca aggcagaagc tgagaagaaa gcggctgctg
     5821 aaaaggctgc agctgataag aaagcggcag cagagaaagc ggcagccgac aaaaaagcag
     5881 cagaaaaagc ggctgctgaa aaggcagcag ctgataagaa agcagcggca gaaaaagccg
     5941 ccgcagacaa aaaagcggca gcggcaaaag cagcagctga aaaagccgct gcagcaaaag
     6001 ctgccgcaga ggcagatgat attttcggtg agctaagctc tggtaagaat gcaccgaaaa
     6061 cggggggagg ggcgaaaggg aacaatgctt cgcctgccgg gagtggtaat actaaaaaca
     6121 atggcgcatc aggggccgat atcaataact atgccgggca gattaaatct gctatcgaaa
     6181 gtaagttcta tgacgcatcg tcctatgcag gcaaaacctg tacgctgcgc ataaaactgg
     6241 cacccgatgg tatgttactg gatatcaaac ctgaaggtgg cgatcccgca ctttgtcaag
     6301 ctgcgttggc agcagctaaa cttgcgaaga tcccgaaacc accaagccag gcagtatatg
     6361 aagtgttcaa aaacgcgcca ttggacttca aaccgtaatc gcgatgttga ctgttccgac
     6421 ggtcaacatc aggcaccggt tgccacgggg ttctggtagt tttgtgtatt ttagtttgtt
     6481 aacattctgc taaattatcg tgggccatcg gtccagataa gggagatatg atgaagcagg
     6541 cattacgagt agcatttggt tttctcatac tgtgggcatc agttctgcat gctgaagtcc
     6601 gcattgtgat cgacagcggt gtagattccg gtcgtcctat tggtgttgtt cctttccagt
     6661 gggcggggcc tggtgcggca cctgaagata ttggcggcat cgttgctgct gacttgcgta
     6721 acagcggtaa atttaatccg ttagatcgtg ctcgtctgcc acagcagccg ggtagtgcgc
     6781 aggaagtaca accagctgca tggtccgcgc tgggcattga cgctgtggtt gtcggtcagg
     6841 tcactccgaa tccggatggt tcttacaatg tcgcttatca acttgttgac actggcggcg
     6901 caccgggtac tgtacttgct cagaactcgt acaaagtgaa caagcagtgg ctgcgttatg
     6961 ctggtcatac cgccagtgac gaagtatttg aaaaactgac cggtattaaa ggtgcgttcc
     7021 gtacccgtat tgcctacgtt gttcagacta acggcggtca gttcccgtat gaactgcgcg
     7081 tatctgacta tgacggttac aaccagtttg tcgttcaccg ttcaccgcaa ccgctgatgt
     7141 ctccggcgtg gtcaccagac ggttctaaac tggcttatgt gaccttcgaa agcggtcgtt
     7201 ccgcgctggt tattcagacg ctggcaaatg gcgctgtacg tcaggtggct tcattcccgc
     7261 gtcacaacgg tgcacctgca ttctcgccag acggcagcaa actggcattc gccttgtcga
     7321 aaaccggtag cctgaacctg tacgtaatgg atttggcttc tggtcagatc cgccaggtaa
     7381 ctgatggtcg cagtaacaat accgaaccga cctggttccc ggacagccag aacctggcat
     7441 ttacttctga ccaggccggt cgtccgcagg tttataaagt gaatatcaac ggcggtgcgc
     7501 cacaacgtat tacctgggaa ggttcgcaga accaggatgc ggatgtcagc agcgacggta
     7561 aatttatggt aatggtcagc tccaacggtg ggcagcagca cattgccaaa caagatctgg
     7621 caacgggagg cgtacaagtt ctgtcgtcca cgttcctgga tgaaacgcca agtctggcac
     7681 ctaacggcac tatggtaatc tacagctctt ctcaggggat gggatccgtg ctgaatttgg
     7741 tttctacaga tgggcgtttc aaagcgcgtc ttccggcaac tgatggacag gtcaaattcc
     7801 ctgcctggtc gccgtatctg tgataataat taattgaata gtaaaggaat cattgaaatg
     7861 caactgaaca aagtgctgaa agggctgatg attgctctgc ctgttatggc aattgcggca
     7921 tgttcttcca acaagaacgc cagcaatgac ggcagcgaag gcatgctggg tgccggcact
     7981 ggtatggatg cgaacggcgg caacggcaac atgtcttccg aagagcaggc tcgtctgcaa
     8041 atgcaacagc tgcagcagaa caacatcgtt tacttcgatc tggacaagta cgatatccgt
     8101 tctgacttcg ctcaaatgct ggatgcacac gcaaacttcc tgcgtagcaa cccgtcttac
     8161 aaagtcaccg tagaaggtca cgcggacgaa cgtggtactc cggaatacaa catctccctg
     8221 ggtgaacgtc gtgcgaacgc tgttaagatg tatctgcagg gtaaaggcgt ttctgctgac
     8281 cagatctcca tcgtttctta cggtaaagaa aaacctgcag tactgggtca tgacgaagcg
     8341 gcgtatgcca aaaaccgtcg tgcggtactg gtttactaag agaattgcat gagcagtaac
     8401 ttcagacatc aactattgag tctgtcgtta ctggttggta tagcggcccc ctgggccgct
     8461 tttgctcagg caccaatcag tagtgtcggc tcaggctcgg tcgaagaccg cgtcactcaa
     8521 cttgagcgta tttctaatgc tcacagccag cttttaaccc aactccagca acaactctct
     8581 gataatcaat ccgatattga ttccctgcgt ggtcagattc aggaaaatca gtatcaactg
     8641 aatcaggtcg tggagcggca aaagcagatc ctgttgcaga tcgacagcct cagcagcggt
     8701 ggtgcagcgg cgcaatcaac cagcggcgat caaagcggtg tggcggcatc aacgacgccg
     8761 acagctgatg ctggtactgc gaatgctggc gcgccggtga aaagcggtga tgcaaacacg
     8821 gattacaatg cagctattgc gctggtgcag gataaatccc gccaggatga cgcaatggtg
     8881 gcatttcaga atttcatcaa aaattaccct gattcaactt acctgccaaa cgccaattat
     8941 tggctgggtc agttaaacta caacaagggt aaaaaagatg atgcggcgta ttattttgct
     9001 tcggtagtga aaaactatcc gaagtcaccg aaggctgcag atgcgatgtt taaagtcggc
     9061 gtcatcatgc aggacaaagg tgacaccgca aaagcgaaag ccgtatacca gcaggttatc
     9121 agtaaatacc ctggtactga tggcgctaaa caggcgcaaa aacgtctgaa cgcgatgtaa
     9181 tgcataacac acgaccagaa gtcgcattat ttctggtcgt gtcgtgcgaa tcataagcag
     9241 ttgagtgatc tacatcgaaa tttttgttgc gctcaagtct gaaatcagta atatatgccg
     9301 ccgttgccac gggatatcaa acaaaccgaa agcaacgaaa aagtgggtcg ttagctcagt
     9361 tggtagagca gttgactttt aatcaattgg tcgcaggttc gaatcctgca cgacccacca
     9421 atcgctaagg tggacgcggt agtaaaacgt gaaggataac gttgcgtcag caacggcccg
     9481 aagggcgaga cgaagtcgag tcatcctgca cgacccacca ctaacatagt tagttgtagt
     9541 atccagcgta gtatcgggtg attagctcag ctgggagagc acctccctta caaggagggg
     9601 gtcggcggtt cgatcccgtc atcacccacc accgggtcgt tagctcagtt ggtagagcag
     9661 ttgactttta atcaattggt cgcaggttcg aatcctgcac gacccaccac taattactgt
     9721 gggttcggtg gaagtagttt gtagtatcca gcgcagtatc gggtgattag ctcagctggg
     9781 agagcacctc ccttacaagg agggggtcgg cggttcgatc ccgtcatcac ccaccactcg
     9841 ggtcgttagc tcagttggta gagcagttga cttttaatca attggtcgca ggttcgaatc
     9901 ctgcacgacc caccaatttt aaacattggt tccgagtaga aatctttcag gtaacacccg
     9961 tatgggtcgt tagctcagtt ggtagagcag ttgactttta atcaattggt cgcaggttcg
    10021 aatcctgcac gacccaccat cctgaatgat taaagcagca taatcccgca aggggtcgtt
    10081 agctcagttg gtagagcagt tgacttttaa tcaattggtc gcaggttcga atcctgcacg
    10141 acccaccaat gcaaaaaagc gccctaaagg cgcttttttg ctattcaggc atctctcaat
    10201 ttcaattctt gaccctgatg acatactccg cgtttgctgc gtaaacaact ctacgtcgga
    10261 ggaataagac gcaaagtgtg gcattcggca tctcatacgt aattcgagtg acttttcccc
    10321 accattcgac tatcttgttt agcatataaa acaaatttca ccgataacag cgaatattac
    10381 gctcatgtcg gttttaacgt taagcctgta aaacgagatg gtaagatgag cgtgatgttt
    10441 gatccagaca cggcgattta tcctttcccc ccgaagccga cgccgttaag cattgatgaa
    10501 aaagcgtatt accgtgagaa gataaaacgt ctgctaaaag aacgtaatgc ggtgatggtt
    10561 gcccactact ataccgatcc cgaaattcaa caactggcag aagaaaccgg tggctgtatt
    10621 tctgattctc tggaaatggc gcgcttcggt gcaaagcatc ccgcttctac gttattagtc
    10681 gctggggtga gatttatggg agaaaccgcc aaaattctca gtccggaaaa aacaattctg
    10741 atgccgacac ttcaggctga atgttcactg gatctcggct gccctgttga agaatttaac
    10801 gcattttgcg atgcccatcc cgatcgtact gtcgtcgtct acgccaacac ttctgctgcg
    10861 gtaaaagcgc gcgcagattg ggtggtaact tcaagcattg ccgtcgaact tattgatcat
    10921 ctggatagtt tgggtgaaaa aatcatctgg gcacccgaca aacatctggg gcgttacgtg
    10981 caaaaacaga cgggcgcaga cattttatgc tggcagggtg cctgcattgt gcatgatgaa
    11041 tttaagactc aggcgttaac ccgcttgcaa gaagaatacc cggatgctgc catactggtg
    11101 catccagaat caccacaagc tattgtcgat atggcggatg cggtcggttc caccagtcaa
    11161 ctgatcgctg ctgcgaaaac attgccacat cagcggctta tcgtggcaac cgatcggggc
    11221 attttctaca aaatgcagca ggcggtgcca gataaagagt tactggaagc accaaccgcg
    11281 ggtgagggtg caacctgccg cagctgcgcg cattgtccgt ggatggccat gaatggcctt
    11341 caggccatcg cagaggcatt agaacaggaa ggaagcaatc acgaggttca tgttgatgaa
    11401 aggctgcgag agagggcgct ggtgccgctc aatcgtatgc tggattttgc ggctacacta
    11461 cgtggataac gaataataag gcgtaacgtt acgctttggg ggaaagatgg atttttttag
    11521 tgtgcagaat atcctggtac atataccaat aggtgcaggc ggttatgatc tctcatggat
    11581 cgaagcggta ggcacgatcg ccgggttgct gtgtattggc cttgccagtc tggagaagat
    11641 cagcaactac ttctttggcc tgatcaacgt caccttgttt ggcattattt tctttcagat
    11701 tcagctgtat gccagcctgc tattacaggt gtttttcttt gccgcgaata tttacggctg
    11761 gtatgcgtgg tcgcgacaaa ccagtcagaa cgaggcggag ttgaaaattc gctggttgcc
    11821 attgccgaag gcgctaagct ggttagcggt ttgcgttgtt tcgattggtc tgatgacagt
    11881 atttatcaat ccggtgtttg catttttgac ccgcgtggca gtcatgatca tgcaagcatt
    11941 aggattacag gttgcgatgc ctgaactaca gccggacgct ttcccgttct gggattcatg
    12001 catgatggtg ttatctatcg tggcaatgat tctgatgacg cgtaagtatg tggaaaactg
    12061 gctgttgtgg gtgattatta acgtgattag cgtcgttatt tttgcacttc agggcgttta
    12121 cgccatgtct ctggagtaca tcatcctgac ctttattgcc ctcaacggca gccggatgtg
    12181 gatcaacagc gcacgcgaaa gaggttcacg cgcgctgtcc cattaatggt gatgatgtga
    12241 atggccagac accccttcat tcaggtggca gtctggccca tgacaaggtt gatattccat
    12301 ctgaatagtg gcgtgctcaa tctgatagtg atccatcagg tagtgttgga tctgatctaa
    12361 caaggcatcg tgatcgtgtg gcgggatcac ctgcacatgc agcgtcatca ccggcttctc
    12421 gcctaccatc catacatgca catggtgcac attgcggact tccgggattt cccggcacat
    12481 acgacgcttc agttcggcga tatccagcga taccggtgca ccttcaagta attcattcac
    12541 gctatctttc aacagtcgcc acgcgctacg cagaaccaga agcgacacca gtattgagag
    12601 aatggggtca gcaggtgtcc agccggtcca gataataatc aacgcggcaa taatagcccc
    12661 aaccgaaccc agcaggtccc cgagcacatg cagtgccgct gctcgcacgt tgaggttttt
    12721 ctcttcgctg ccgtgatgaa gtaaccagaa agaaagtata tttgccagca gcccagccac
    12781 ggcaattgcc atcatcatgc cgccctcgac cggacgcgga gtgcggaagc gttctatcgc
    12841 ctcccagaca atcaaaatag taatcaccac cagggcgatg gcattcacaa acgccgcgag
    12901 cgtggtcaat cttagccaac cgaaagtgtg gcgaatggtg ggaggacgac gggaaaattg
    12961 cactgcgagt agggcgaaaa gcagggcggc agtatcggtc aacatatgac ccgcatcggc
    13021 cagcaatgcc agagaaccgg aaagaaaacc accaatgact tctacgagca taaacccggc
    13081 agtcacgccg aaagcatata acaagcgacg agcattatta tcttctggca ggtgtgagga
    13141 agtgtgtgag tgtgagtgcg ccatgacgag tcatccctta attattctca cttaacttta
    13201 tgacatcata ctgcttttag aagggaaaaa ttaaaaggga gagactccgc tctcccatta
    13261 ttggctattt cgcagggtta ctgcgtggta ccgtcggttt tggtatcgac atcattgttg
    13321 atgccatcac cggtttggac ttttttattg atatccgggc agcgaccatc tttgcacatg
    13381 gtgttcttgt gctcttcatc tttggtcatg ccgtcgttgt tcattgaaga accatccgga
    13441 tgcagcattg tgcctccaga accggtattt accccgttat tgtcgacgtt atttggcgcg
    13501 acattttcac gggcgtcagg ggctacctgg cccgcatcag ctgcggcgtt tgcctggccg
    13561 ttattgcttt gcgctccgct atcggcagcc agtgcggcac cgctggcaag gcttagagtg
    13621 gcagtcaaaa atagtgtggc cagttttgtc attttcatag gatgctcctg ttatggtcgt
    13681 tatgtcggat aacctcttcc aacagtgcat ttgcaggtga atataaggca atggtttaag
    13741 atttcagcca ggcaaagata cccagcagag aatcttgaaa taattaacaa acaaaggagt
    13801 tacagttaga aattgtagga gagatctcgt ttttcgcgac aatctggcgt ttttcttgct
    13861 aattccagga ttaatccgtt catagtgtaa aaccccgttt acacattctg acggaagata
    13921 tagattggaa gtattgcatt cactaagata agtatggcaa cactggaaca gacatgaatt
    13981 atcagaacga cgatttacgc atcaaagaaa tcaaagagtt acttcctcct gtcgcattgc
    14041 tggaaaaatt ccccgctact gaaaatgccg cgaatacggt cgcccatgcc cgaaaagcga
    14101 tccataagat cctgaaaggt aatgatgatc gcctgttggt ggtgattggt ccgtgctcaa
    14161 ttcatgatcc tgtcgcggcg aaagagtatg ccactcgctt gctgacgctg cgtgaagagt
    14221 tgcaagatga gctggaaatc gtgatgcgcg tctattttga aaagccgcgt actacggtgg
    14281 gctggaaagg gctgattaac gatccgcata tggataacag cttccagatc aacgatggtc
    14341 tgcgtattgc ccgcaaattg ctgctcgata ttaacgacag cggtctgcca gcggcgggtg
    14401 aattcctgga tatgatcacc ccacaatatc tcgctgacct gatgagctgg ggcgcaattg
    14461 gcgcacgtac caccgaatcg caggtgcacc gcgaactggc atcagggctt tcttgtccgg
    14521 tcggcttcaa aaatggcacc gacggtacga ttaaagtggc tatcgatgcc attaatgccg
    14581 ccggtgcgcc gcactgcttc ctgtccgtaa cgaaatgggg gcattcggcg attgtgaata
    14641 ccagcggtaa cggcgattgc catatcattc tgcgcggcgg taaagagcct aactacagcg
    14701 cgaagcacgt tgctgaagtg aaagaagggc tgaacaaagc aggcctgcca gcacaggtga
    14761 tgatcgattt cagccatgct aactcgtcaa aacaattcaa aaagcagatg gatgtttgtg
    14821 ctgacgtttg ccagcagatt gccggtggcg aaaaggctat tattggcgtg atggtggaaa
    14881 gccatctggt ggaaggcaat cagagcctcg agagtgggga accgctggcc tatggtaaga
    14941 gcatcaccga tgcctgcatt ggctgggatg ataccgatgc tctgttacgt caactggcga
    15001 atgcagtaaa agcgcgtcgc gggtaattta tagtcgaatc gccatgactg tacgtttgca
    15061 gccctgataa gacgcgcagc gtagcatcag gcaatgtgct ccattgttag caacgacaaa
    15121 caaaaaagcc gactcacttg cagtcggctt tctcattttt aaacgaatga cgtttacttc
    15181 gctttaccct ggttcgcaac ggctgctgct ttcgccgcga tctcgtcagc attacccaga
    15241 taatagcgtt tcagcggttt gaagttctcg tcaaactcat aaaccagcgg cacgccggtc
    15301 gggatattca gctcaagaat ctcttcttcg ctcatgttat caagatattt caccagcgca
    15361 cgtaacgagt taccgtgtgc agcgatgatt acgcgctcac cgctcttcat acgcggcaga
    15421 atggtttcat tccagtaagg gatcacgcgg tcaatggtca gcgccaggct ttccgtcagc
    15481 ggcagttctt tctcgctcag cttcgcgtaa cgtggatcgt gacccggata acgctcatca
    15541 tctttagtca gttccggcgg agtcactgca aaaccacgac gccactgttt cacctgctcg
    15601 tcgccatact tttcagcggt ttctgctttg ttcagaccct gcaacgcacc gtagtgacgt
    15661 tcgttcagtt tccaggattt ctcaacgggc agccatgcct gatccagttc gtccagcaca
    15721 ttccacaggg tatggatagc gcgtttcagc acagaggtgt aagcaaagtc aaagctgtaa
    15781 ccttcctctt tcagcagctt acctgctgct tttgcttcgc ttacgccttt ctcagacaga
    15841 tccacgtcgt accaaccggt gaaacggttt tctttgttcc actgactttc gccatgacga
    15901 accagaacca gcttagttac agccatatac ttactcctca aatcatcttt taatgataat
    15961 aattctcatt atattgccgt gacgaagcaa cagcaatgct tacgcataac catagcgaaa
    16021 atagtgtcgc agtgtaaggt tgttgtgaat attgagttgc aaatatgtcg gtgtttgctg
    16081 gtgatttgaa caatatgaag ataaagccct catgacgagg gcttaacatt actgagcaat
    16141 aaactgatat tccgtcaggc tggaatactc ttcgccagga cgcaggaaac agtccggttg
    16201 cggccattca gggtggttcg ggctgtccgg cagaaactcg ctttccaacg ccagcccttg
    16261 ccagtcggtg taaggttcgg ttccccgcga cggtgtgccg ccgaggtagt tgccggagta
    16321 gaattgcaga gccggagcgg tggtgtagac cttcagctgc aatttttcat ctgctgacca
    16381 gacatgcgcc gccactttct tgccatcgcc tttggcctgt aacaagaacg cgtgatcgta
    16441 acctttcact ttgcgctgat cgtcgtcggc aagaaactca ctggcgatga ttttggcgct
    16501 gcggaaatca aaagacgttc cggcgacaga tttcaggccg tcgtggggaa tgccgccttc
    16561 atcaaccggc agatattcgt ccgccagaat ctgcaacttg tgattgcgca cgtcagactg
    16621 ctcgccgtca agattgaaat agacgtgatt agtcatattc accgggcaag gtttatcaac
    16681 tgtggcgcga taagtaatag agatacggtt atcgtcggtc agacgatatt gtaccgtcgc
    16741 tccgagatta cccgggaagc cctgatcgcc atcatctgaa ctcagggcaa acagcacctg
    16801 acgatcgttc tggttcacaa tctgccagcg acgtttgtcg aacccttccg ggccgccgtg
    16861 cagctggtta acgccctgac ttggtgaaag cgtcacggtt tcaccgtcaa aggtataacg
    16921 gctattggcg atacggttgg cataacgacc aatagaggcc cccagaaatg cggcctgatc
    16981 ctgatagcat tccgggctgg cacagccgag cagcgcctcg cggacgctgc catcggaaaa
    17041 cggaatacgg gcggaaagta aagtcgcacc ccagtccatc agcgtgacta ccatccctgc
    17101 gttgttacgc aaagttaaca gtcggtacgg ctgaccatcg ggtgccagtg cgggagtttc
    17161 gttcagcact gtcctgctcc ttgtgatggt ttacaaacgt aaaaagtctc tttaatacct
    17221 gtttttgctt catattgttc agcgacagct tgctgtacgg caggcaccag ctcttccggg
    17281 atcagcgcga cgatacagcc gccaaatccg ccgccggtca tgcgtacgcc acctttgtcg
    17341 ccaatcacag ctttgacgat ttctaccaga gtgtcaattt gcggcacggt gatttcgaaa
    17401 tcatcgcgca tagaggcatg agactccgcc atcaactcgc ccatacgttt caggtcgcct
    17461 tgctccagtg cgctggcagc ttcaacggtg cgggcgtttt cagtcaggat atgacgcacg
    17521 cgtttcgcca cgattgggtc cagttcatgc gcaacagcgt tgaactcttc aatggtgaca
    17581 tcgcgcagcg ctggctgctg gaagaaacgc gcaccagttt cgcactgttc acgacgggtg
    17641 ttgtattcgc tgccaaccag ggtacgtttg aagttactgt taatgatgac gacagccaca
    17701 cctttcggca tggaaactgc tttagtcccc agtgagcggc agtcaatcag caaggcatga
    17761 tctttcttgc cgagtgcgga aattagctga tccatgatcc cgcagttaca gccaacaaac
    17821 tggttttctg cttcctgacc gttaagcgcg atttgtgcgc cgtccagcgg caaatgataa
    17881 agctgctgca atacggttcc gaccgcgact tccagtgaag cggaagaact taacccggca
    17941 ccctgcggca cattgccgct gatcaccatg tccacgccgc cgaagctgtt gttacgcagt
    18001 tgcagatgtt tcaccacgcc acgaacgtag ttagcccatt gatagttttc atgcgcgaca
    18061 atggtcgcat cgagggaaaa ctcgtcgagc tgattttcat aatcggctgc catcacgcga
    18121 actttacggt catcgcgtgg tgcacaactg atcacggttt gataatcaat cgcgcagggc
    18181 agaacgaaac cgtcgttgta gtcggtgtgt tcaccaatca aattcacgcg gccaggcgcc
    18241 tgaatggtgt gagtggcagg gtagccaaat gcgttggcaa acagagattg tgttttttct
    18301 ttcagactca tttcttacac tccggattcg cgaaaatgga tatcgctgac tgcgcgcaaa
    18361 cgctctgcgg cctgttctgc ggtcaggtct cgctgggttt ctgccagcat ttcataacca
    18421 accataaatt tacgtacggt ggcagagcgc aacagaggcg gataaaagtg cgcgtgcagc
    18481 tgccagtgtt gattctcttc gccattaaat ggcgcgccgt gccagcccat agagtagggg
    18541 aaggagcact ggaagaggtt gtcataacga ctggtcagct ttttcaacgc cagtgccaaa
    18601 tcgctgcgct gggcgtcggt caaatcggtg atccgtaaaa cgtgggcttt gggcagcagt
    18661 agcgtttcga acggccaggc agcccagtaa ggtacaacgg ctaaccagtg ttcggtttcg
    18721 acaacggtac ggctaccgtc tgccagctcg cgctgaacat aatccaccag cattggcgat
    18781 ttctgttcgg cgaaatattc tttttgcaga cggtcttcgc gctcagcttc gttaggcagg
    18841 aagctatttg cccaaatctg tccgtgcgga tgcgggttag agcagcccat cgccgcgcct
    18901 ttgttttcaa agacctgcac ccatgggtac gttttcccca gttctgcggt ttgctcctgc
    18961 caggttttga cgatttccgt caatgccgca acgctgagtt ctggcaacgt tttgctgtga
    19021 tccggtgaaa agcagatcac ccgactggtg ccacgcgcgc tctggcaacg catcagcgga
    19081 tcgtgacttt ccggcgcatc tggtgtgtca gacatcaaag ccgcaaagtc attagtgaaa
    19141 acgtaagtcc cggtgtaatc ggggttttta tcgcctgtca cccgcacatt acctgcgcag
    19201 aggaagcaat ctggatcatg agcaggtaac acctgtttgg ctggcgtttc ctgcgccccc
    19261 tgccaggggc gcttagcgcg gtgcggtgaa accagaatcc attgcccggt gagcgggttg
    19321 tagcggcgat gtggatgatc aacgggatta aattgcgtca tggtcgttcc ttaatcggga
    19381 tatccctgtg gatggcgtga ctgccagtgc caggtgtcct gcgccatttc atcgagtgtg
    19441 cgcgttacgc gccagttcag ttcacggtcg gctttgctgg cgtccgccca gtaggccgga
    19501 aggtcgccct cgcgacgcgg tgcaaaatga taattaaccg gtttgccgca ggctttgctg
    19561 aaggcattaa ccacgtccag cacgctgctg cctacgccag cgccgaggtt atagatgtgt
    19621 acgcctggct tgttcgccag tttttccatc gccacgacgt gaccgtccgc cagatccatt
    19681 acgtggatgt aatcgcgtac gccagtaccg tcttcggtcg gataatcgtt accaaaaatc
    19741 gccagcgagt cgcgacggcc tacagcaacc tgggcgatgt atggcatcag gttattcgga
    19801 atgccttgcg gatcttcgcc catatcgccc gacggatgcg cgccaaccgg gttgaagtag
    19861 cgcagcaggg caatgctcca gtccggctgg gctttttgca gatcggtgag gatctgttcc
    19921 accatcagtt tgcttttgcc gtaagggctt tgcggtgtgc cggtcgggaa gctttcaacg
    19981 tatggaattt tgggctgatc gccatagacg gtggcggagg agctaaaaat aaagtttttg
    20041 acgttcgcgg cgcgcatggc gctaatcagg cgcagggtac cgttgacatt gttgtcgtaa
    20101 tattcaagcg gtttttgtac cgattcgcca acggctttca gcccggcgaa gtggatcaca
    20161 gtgtcgatag cgtgatcgtg caggatctcg gtcatcaacg cttcgttacg gatatcgcct
    20221 tcaacaaacg ttggatgttt gccgcctaaa cgctcgataa caggcagtac gctgcgctta
    20281 ctgttacaga ggttatcaag aatgatgaca tcatgaccgt tttgcagtaa ttgcacacag
    20341 gtatgacttc caatgtaacc gctaccaccg gtaaccagaa ctctcataat tcgctccatt
    20401 aggcttatgg tatgaaataa ccatagcata acaaagatgc gaaaagtgtg acatggaata
    20461 aattagtgga atcgtttaca caagaattta gccgtttttt atgcgccatt aatggattat
    20521 aaaacagagg gtttatgaat gattgagctt tttatctgaa aaaagtcgcg gtttcatgcc
    20581 tggatgcgtc gaactgtagg ccggagaggg cgcttaggcc gcctccggta cagtcagcat
    20641 taccgactca gtttattttt gtcagcgcat agcgataaaa gtcaccgtcc ggcacgaact
    20701 caagacgatg ggtaatacag gcaggcgcat cttcagcatg gtgcgaaaca aacaacaatt
    20761 gcgtttcacc ttcgctaatc agcacatcaa caaaccggcg gataagctgg cgattgagcg
    20821 gatcaagccc ctgtagtggt tcatcgagaa taagcaacgt cggatgtttc accagcgcgc
    20881 ggacgatcag cgccagacgc tgctgtcccc aggaaagact atggaacgga gcgtcagccg
    20941 tgcgtttatc aatgccgaga atatccagcc actgctgcac cagtttttgc tggcgatccg
    21001 aaacggcctg ataaatgcca atcgaatcaa aatagccaga aaggatcaca ttacgcacgg
    21061 tagtgctgac ccggtaatcc agatgcaaac tactgctgac gtaaccgata tgctttttga
    21121 tatcccagat ggtttcgccg ctgccgcgac gtcgtccgaa aagcgtcaaa tcgttgctgt
    21181 aaccttgcgg atgatcgcca gtaatcaggc ttaataacgt cgattttccc gcgccatttg
    21241 gcccgacaat ttgccagtgc tcgcctggat tcacctgcca gctaaggtta ttaagaatgg
    21301 ggcgatcgtt ataagaaacc acgccgttgt tcagcacaat gcgcggttcg ttggcgggta
    21361 aggcgtgacg tgctgaaggt tcatccggct ccggcagttg cacaccttca agctgttcgc
    21421 tatgtgccag ttgcgcgacg agtgcttgct ggagcagttc ctctttagcg cccgtttccg
    21481 ctaacgtaca atccgccagc acgccagcaa actggacaaa ttccgggatc tcatcgaagc
    21541 gattgagcac cagtaccaga gtaataccgg actgatgtaa cgaggcgagt agctcagcca
    21601 gctgctgacg tgaggcaaca tccaggccat cgaacggctc atcaagaatc aacaagtcag
    21661 gctccgacat cagcgcctga cacagcaggg tttttcgcgt ctcgccagtg gaaaggtatt
    21721 taaagcgtcg gtcgaggagg gcggtaatac cgaactgctg cgccagttgc atgcaacgcg
    21781 gtgcatcctt tacttcatcc tgaatgattt cagccgtggt acgcccggtg tcatcttcgc
    21841 cagggccgag catatcggtg ttattccgct gccattcgtc gctgacgagc ttttgcaatt
    21901 gctcgaagga gagacgagtg atgtgggaaa actggctttg ccgttcacct ttcaaaagcg
    21961 gaagttcccc cgcctgcgcg cgggccagcg ccgatttccc gcttccattc gaaccgacaa
    22021 acgcccaact atcacccgcg tttaacgtta gctgaggcaa ttgcagcgtt tttgtgtcgc
    22081 taagacgaaa cgtgccttgc aaaatttgca acgatgacat tttatatccc attttgtgca
    22141 gcgattactg acagggatac gtgtttcata acaaattgtc aacacgctta gcacagcgtg
    22201 gcgataatca cgctgtcggc attaaagtag gccgtgacat tctgtccttg ctgaagagaa
    22261 gtcgcttcat ttaccggcac tgtggcgcac agtgtttgcc cgtcgggtag cgccattaat
    22321 acttcgcact gctctgcgcc gcgctcaata tggctaataa tacccggtaa ttggttgtcg
    22381 gcgttttgtg cgaccgcttc gtcctgagta atgcctaccc acggcgcttt tagtaatatc
    22441 aacacttctt tgccttcatc cagccccaga cgcgcgccgc tttgtgcggt aattgcgact
    22501 ttcaggcgtg tttttccgtc agccagtaag acatcaacat gctgttgaac gtcatcatga
    22561 tcgcgggcgg tgatggtgcc aaaccattgg ttacgggcgc tggtttgcag tgaaaaacgt
    22621 gagatcgcgg ccagcaggct gttcagcggc agggcgtcat cgtcacttaa cacatcaaag
    22681 gctttttgct ggatttgcgc cagtaagtca tagagctgaa tcagtcgctg accatagcgg
    22741 gtcagtactg cgccgcctcc acctttaccg cctgttgcgc gctcaaccag aatatgctca
    22801 cttaactgat tcatctcgtt aatggcatcc catgcgcttt tatagctgat accggcatct
    22861 ttcgctccct ggctaatgga accggaaagc gcaatgtgtt ttagtagcga aatgcggcgc
    22921 gggtcggcga ataatttttg ttggagctta agggtgagaa ggatttcggc ctgcataaca
    22981 atatcctgga aaaagtctta ttgtgacgga aaacgaacgc cacgcaaagc tgaccgcaca
    23041 aaaggggagt gcttttcttt gcttagcggt tagaatagtc tcatgactat atctggagtt
    23101 gaccatgtta gagttattaa aaagtctggt attcgccgta atcatggtac ctgtcgtgat
    23161 ggccatcatc ctgggtctga tttacggtct tggtgaagta ttcaacatct tttctggtgt
    23221 tggtaaaaaa gaccagcccg gacaaaatca ttgaatccct gaatgcccgc ttagtcgggc
    23281 atttcttttt ctcaacctcc tgcttttcct gccgatattt tttctcatct acctcacaaa
    23341 ggttagcaat aactgctggg aaaattctga gttagccgtt atattgtcgc ctacataacg
    23401 ttagattaag gggttaccaa tggctcgtaa atggttgaac ttgtttgccg gggcggcact
    23461 ctctttcgct gttgctggca atgcactggc agatgaaggg aaaatcacgg tgttcgccgc
    23521 cgcatcactg actaacgcaa tgcaggacat tgctacgcag tataaaaaag agaaaggcgt
    23581 ggatgtggtt tcttctttcg cttcgtcatc tactttggcc cgtcagattg aagcgggtgc
    23641 gcctgcggat ctgtttattt ctgccgatca gaaatggatg gattatgcgg ttgataaaaa
    23701 agcgatcgat acggcttcgc gccagacact gctcggcaat agcctggtcg ttgtagcacc
    23761 gaaagccagc gagcagaaag atttcaccat cgacagcaaa accaactgga cttcgctgct
    23821 gaatggcggt cgtctggcgg ttggcgatcc ggaacatgtt cccgctggca tttatgcaaa
    23881 agaagcactg caaaaactgg gcgcatggga tacgctctcc ccgaaactgg ctccggcgga
    23941 agatgttcgt ggcgcgctgg cgttggtcga acgcaatgaa gcgccgctgg gtattgttta
    24001 cggttctgac gcagttgcca gcaaaggggt aaaagtggtt gccaccttcc cggaagattc
    24061 acataaaaaa gtggaatatc cggttgctgt tgtggaaggg cataacaatg cgacagtgaa
    24121 agcattttat gattatttga aagggccgca ggctgcggaa atctttaaac gttacggatt
    24181 tacaaccaag taatgatact gactgatcca gaatggcagg cagttttatt aagcctgaaa
    24241 gtttcttccc tggctgtgct gtttagcctg ccgtttggga tcttttttgc ctggttactg
    24301 gtgcgttgca cgtttccggg caaagctctg ctcgacagcg tactgcatct accgctggtg
    24361 ttaccgcccg tggtcgtcgg ttacttatta ttagtttcga tgggacggcg cggatttatc
    24421 ggtgaacgtc tgtatgactg gtttggcatt accttcgcct ttagctggcg cggcgcggtt
    24481 ctcgctgccg ccgttatgtc gtttccgctg atggtgcggg caattcgtct ggcgctggaa
    24541 ggtgttgata tcaaactgga acaggccgca agaacactgg gtgccgggcg ctggcgcgtt
    24601 ttctttacta tcacgttacc gctgacctta ccgggcatta ttgttggtac ggtactggct
    24661 tttgctcgtt ctctcggtga gtttggtgca accatcacct ttgtgtcgaa cattcctggt
    24721 gaaacgcgaa ccattccttc tgccatgtat accctgatcc agacccccgg cggggaaagt
    24781 ggcgcggcga gactgtgcat tatttctatt gcgctggcga tgatctccct gttgatttca
    24841 gaatggctgg ccagaatcag ccgtgaacgg gcagggcgct aatcatgctg gaactgaatt
    24901 tttcccagac gttgggcaac cattgcctga ccattgatga aacgctgccc gccaatggca
    24961 taactgctat ctttggcgtc tccggtgccg gaaaaacttc gctgattaac gccatcagtg
    25021 gactgacacg tccgcaaaaa gggcggattg tcctcaatgg tcgggtacta aatgatgccg
    25081 aaaaaggtat ctgcctgtcg ccggaaaagc gtcgcgttgg ctatgttttt caggatgcgc
    25141 ggctgttccc gcattacaaa gtgcgtggca atctgcgcta cggcatggcg aaaagcatgg
    25201 tcaatcagtt cgataagctg gtggcgcttt taggcattga accgttgctt gaacgtttac
    25261 caggcagcct gtccggaggc gaaaaacagc gcgtggcgat tggtcgggct ttgctgacag
    25321 caccggaatt gctgttgctg gatgaaccgc tggcgtcact ggatattccg cgtaaacgcg
    25381 aactgttgcc ttatctgcaa cgtctgacgc gggaaatcaa cattccgatg ttgtatgtca
    25441 gccattcgct ggatgaaatc ctccatctgg cagacagagt gatggtactg gaaaacggtc
    25501 aggtgaaagc ctttggcgcg ctggaagaag tctggggtag tagcgtgatg aatccgtggc
    25561 taccgaaaga gcaacaaagt agcattctga aagtgacagt acttgagcat catccgcatt
    25621 acgcgatgac cgcgctggcg ctgggcgatc agcatttgtg ggtcaataag ctggacgaac
    25681 cgctgcaagc tgcgctacgc atccgcattc aggcttccga tgtctcgctg gttttacaac
    25741 cgccacagca aaccagcatt cgtaacgtat tgcgggcaaa agttgttaat agttatgacg
    25801 acaacggcca ggtggaagtg gagctggaag tcggcggtaa aacgctgtgg gcgcgtatca
    25861 gcccgtgggc cagggatgaa ctggcgatca aacctggcct gtggctgtat gcgcaaatta
    25921 aaagtgtgtc gataaccgcc tgattaaatc aggtggctat aaatgaactg ggcaatgctg
    25981 tcggtggtgt tatcaccaat cacaatgttg gcgcgcgctt ttactgcgtc atcggcgttg
    26041 cccatcgcca cgcctgtacc agcggcttcc agcatactaa tatcattgaa gttatcgccg
    26101 aaggccacga cgttttccat cgaccagcct tgcgcctcaa cccatttcgt caaacgttta
    26161 cctttgctgt tgccgccgcg tgcaatatca acctgatcgt gccaggacca ttcacactcc
    26221 agtcccagtt catgttcgac atgcttacca aaattctgca attgcggcag gtcatcgtgc
    26281 gtcagggcga acttccatac ggcgttaact tgttgcgccg tttcagccag agaagcgact
    26341 tgtgtgaaag tcggacgctg ttccggcggc agggtttgcg cccagttaga cgtgcgaatg
    26401 acatgtccgg tcgggtgctc ataaaccatc gcatcatcca catacatcag accgtgaatg
    26461 tggtgttcat tcagcatctc aatgagttgc agggctttgt taacaggcat tgggtccgct
    26521 tccagcacag tttttgcatg ataatcatac aaataggtgc cattacagca aatagcaggt
    26581 gtatccagcg ccagcgcctg ataaaaagga tgaatagcga cgtgatggcg acctgtgacg
    26641 atgattaatt gatagcctgc ttcgcgagcg cgggccaggg cttctatcga tgatggaagc
    26701 aaggtctttt tcggggtcaa taaggtgccg tctaagtcga gagcaatcac gcgtgtggtc
    26761 atggcgtatt ccagattaag gttaagaatt ttcgtctgcg cgaatggtac accgatacca
    26821 ctatcggaca aaattctgcg ttttaattca gcattcaccg ccaaaagcga ctaattttag
    26881 ctgttacagt cagttgctaa atgcaaagga gcattcatga agcaaacagt ttatatcgcc
    26941 agccctgaga gccagcaaat tcacgtctgg aacctgaatc atgaaggcgc actgacgctg
    27001 acacaggttg tcgatgtgcc ggggcaggtg cagccgatgg tggtcagccc ggacaaacgt
    27061 tatctctatg ttggtgtgcg ccctgagttt cgcgtcctgg cgtatcgtat cgccccggac
    27121 gatggcgcgc tgacctttgc cgcagagtct gcgctgccgg gtagtccgac gcatatttcc
    27181 accgatcacc aggggcagtt tgtctttgtc ggttcttaca atgcgggtaa cgtgagcgta
    27241 acgcgtctgg aagatggcct gccagtgggc gtcgtcgatg tggtcgaggg gctggacggt
    27301 tgccattccg ccaatatctc accggacaac cgtacgctgt gggttccggc attaaagcag
    27361 gatcgcattt gcctgtttac ggtcagcgat gatggtcatc tggtggcgca ggaccctgcg
    27421 gaagtgacta ccgttgaagg ggccggcccg cgtcatatgg tattccatcc aaacgaacaa
    27481 tatgcgtatt gcgtcaatga gttaaacagc tcagtggatg tctgggaact gaaagatccg
    27541 catggcaata tcgaatgtgt ccagacgctg gatatgatgc cggaaaactt ctccgacacc
    27601 cgttgggcgg ctgatattca tatcaccccg gatggtcgtc atttatacgc ctgcgaccgt
    27661 accgccagtc tgattactgt tttcagcgtt tcggaagatg gcagcgtgtt gagtaaagaa
    27721 ggcttccagc caactgaaac ccagccgcgc ggcttcaatg ttgatcacag cggcaagtat
    27781 ctgattgccg ccgggcaaaa atctcaccac atctcggtat acgaaattgt tggcgagcag
    27841 gggctactgc atgaaaaagg ccgctatgcg gtcgggcagg gaccaatgtg ggtggtggtt
    27901 aacgcacact aaccgctgat ttacccggcg cagtctcttc tgcgccggtg tattaaccta
    27961 tctcctgtaa cgcatgtctc tggcgttcga cgatattggt ccacaaattg tctttatcgt
    28021 cagtccacaa attaatcagc aaggcaaaaa agcgttcagc tgccggagaa agcacggcat
    28081 ctttgcggcg aataatcccc aatgtccgac gtataaccgg ttccaccagc gggataccaa
    28141 taatcgaaga atagggcgca tggggcattg ccagaccagg aagcgccgaa atccccagtc
    28201 ccgcctccac cagccctaat gacgtcgaaa gatgacgcac ttcgtaaaac cagtccagct
    28261 tccagggctt gtcggctagc tgttgttcta tcaatagtcg gttgccgctg gaggagcgta
    28321 cgccaatcat tttgtaaccc actaactcct gccattctac aagctgcttt ttggccaatg
    28381 gatggtcacg ccgacaggcc agcacgaacg gctcgttgac cagtggggta aaatcaatgg
    28441 atgaatttgt gacgttgttc atgtttatgc caaagtctga ttcgttacac agcaccgatt
    28501 ccatgcaatt attcgtgcct tgttccagaa tccgcacttt aatattggga tacagctcat
    28561 taaatttacc gattgccagc ggtaaaaaat aaaatactgc ggtcggaata cacgctaacg
    28621 tcaccatacc acgatgataa gcattcatat cacgaatatt aaaaagcgtt tcatcgaatt
    28681 tttttattaa ttcccgcgcc tccgggagca acctttttcc tgcttttgtt aacgtaacct
    28741 tgcgagttgt acgctcaaat agttgaatgt gtaaatcttc ctccatcttt tttattcttc
    28801 gcgttaatgc aggttgcgta atattgagta atttagcagc attgttaaat gaactggact
    28861 ctgccagtat gacaaatgcc ttcatacttg ataattcatg cttcattatt actccggaaa
    28921 atggaagcga cgattttggg tgactggcag ttaaaaattt taactgcatt tagccaactt
    28981 aaattaatga aaaaatgtta ttaatcgttg agctaaagtc attagagatg ctttgccctt
    29041 aatgtaacca tatcgcaata agttatgttt ttaaattgag ggcattatta tgaaaaaaat
    29101 accctgcgtg atgatgcgag gtggaacctc gaggggcgcg ttcctgttag cggaacatct
    29161 acccgaagat caaacgcagc gcgataaaat attgatggca attatgggtt ccggtaacga
    29221 tctggaaatt gacggtattg gcggcggtaa tccgctgacc agtaaagtcg ccattattag
    29281 ccgctccagc gatccgcgtg cagatgtcga ttatctgttt gctcaagtta tcgtccatga
    29341 gcaacgtgtc gataccacgc ctaactgcgg caatatgctg tctggcgttg gggcattcgc
    29401 cattgaaaat ggtttgattg cagcgacttc gccagttacc cgcgtacgta tccgcaacgt
    29461 caatacgggt acgtttatcg aagctgatgt gcaaacgcca aatggtgttg tcgagtacga
    29521 gggtagcgcc agaattgacg gcgtaccggg tactgccgca ccggttgcgc tcactttcct
    29581 gaatgccgcc ggaacgaaaa ccggaaaagt cttcccgacg gataatcaga ttgattattt
    29641 tgacgatgtc ccggtgacct gtatcgatat ggcgatgcca gttgtcatta ttcctgctga
    29701 atatctggga aaaacaggct atgaattacc ggcagagctg gatgccgaca aagcattatt
    29761 agccagcatt gaatctatcc gtctacaagc gggtaaagca atgggcttag gcgatgtcag
    29821 taatatggtt atccctaaac ctgtacttat ttctccagcg cagaaaggcg gggcaattaa
    29881 tgtgcgttat tttatgccgc attcttgcca tcgcgctttg gcgataaccg gtgctattgc
    29941 tatttcctgt agttgtgcat tggaaggcac cgtcacccga caaatcgtcc cttctgtagg
    30001 atacggcaat atcaatattg aacaccccag tggtgcgctc gacgttcatt taagtaatga
    30061 aggtcaggat gccacgacgt tacgcgcatc tgttattcgg acgacaagaa aaatattttc
    30121 cggtgaagtt tatcttccct gaaaaatttt gttgtcgtga taatgacaat caataaagga
    30181 attctgtatg agtcatacag aaagaacagg atattaaatg aataaaaaat cgttatggaa
    30241 gctaattctg atattagcga tcccatgtat tattggcttt atgccagctc cggcaggatt
    30301 aagcgaactg gcgtgggtgc tttttggtat ttacctggcg gccattgtgg ggctggttat
    30361 caagcctttc ccggaacctg tcgtactgtt aattgccgtc gccgcatcga tggtagtggt
    30421 gggtaactta tcaggtgggg aatttaaaac caccgccgta ttaagcggtt actcttccgg
    30481 taccacctgg ctggtgtttt ctgcgtttac tttaagcgcc gcgtttgtaa ccacaggctt
    30541 aggtaaacgt attgcctatc tgctgattgg taaaattggt agcactaccc tgggtctggg
    30601 ttacgttacg gtatttctcg atctggtact ggctccggca acaccgtcta acaccgcgcg
    30661 tgcgggcggc atcgtgttac cgatcatcaa cagcgtggca gtggctttgg gatcagaacc
    30721 ggaaaaaagt ccgcgtcgtg ttggacatta cctgatgatg tccatttaca tggtcactaa
    30781 aaccaccagc tatatgttct ttaccgcaat ggcggggaac attctggcgc tgaaaatgat
    30841 caacgacatt ctgcacctgc aaattagctg gggtggatgg gcgctagccg ccggattgcc
    30901 gggcatcatt atgctgctgg tcaccccgct ggtgatttac accatgtatc caccagaaat
    30961 taagaaggtg gataacaaaa ccatcgctaa agcgggcctt gccgaactgg gaccgatgaa
    31021 aatccgcgaa aaaatgctgc tcggtgtctt tgtgctggcg ctgctgggct ggattttcag
    31081 taagtctctg ggggttgatg aatccaccgt ggcaatcgtt gttatggcaa ctatgctgct
    31141 gctgggtatc gttacctggg aagacgtggt taaaaataaa ggcggctgga ataccttaat
    31201 ctggtacggc ggtattatcg gcttaagctc cttattatcg aaagttaaat tcttcgaatg
    31261 gttagctgaa gtctttaaaa ataacctggc atttgatggt cacggtaacg ttgctttctt
    31321 cgttattatt ttcctcagca tcatcgtgcg ttatttcttc gcttctggta gtgcctatat
    31381 cgttgccatg ttaccggtat ttgccatgct ggcgaatgtc tccggcgcac cgttaatgtt
    31441 aaccgcgctg gcactgttgt tctctaactc ctatggcggc atggttactc actatggcgg
    31501 cgcggcaggt ccggtcatct ttggcgtggg ttacaacgat attaaatcct ggtggttggt
    31561 cggtgcggta ctgacgatat taaccttcct ggtgcatatc accctcggcg tgtggtggtg
    31621 gaatatgctg atcggctgga acatgctgta aatatactcg tcatacttca agttgcatgt
    31681 gctgcgtctg cgttcgctca ccccagtcac ttacttatgt aagctcctgg ggattcactc
    31741 gcttgtcgcc ttcctgcaac tcgaattatt tagagtatat ccatttatta tctttctgcg
    31801 cacttcacgg tgcgcagata tctggagcat ttgatgatca agttatctga aaaaggcgtg
    31861 tttctcgcca gcaataacga aataattgcc gaagaacatt tcaccggcga aataaaaaaa
    31921 gaagaagcaa aaaaaggcac tattgcctgg tctattctct cttctcataa tacgtccgga
    31981 aatatggata aacttaaaat taagtttgat tcattagcct ctcacgatat tacctttgtt
    32041 ggtattgtac agaccgctaa agcgtccggt atggaacgtt tcccgctgcc gtatgtgctg
    32101 accaactgcc ataactcact ctgtgctgtg ggcggcacca ttaacggtga tgaccatgtt
    32161 tttggtttat cggcggccca gcgttatggc ggtatttttg tgccaccgca tattgcggtc
    32221 atccatcaat atatgcgtga aatgatggcg ggcggcggca aaatgatcct cgggtcagac
    32281 agccataccc gttacggtgc gttagggaca atggcggttg gcgagggcgg cggcgagttg
    32341 gtaaaacagc tgcttaatga cacctgggat atcgactatc cgggcgtggt tgcggtgcat
    32401 ctgaccggaa aacctgcgcc gtatgtgggg ccgcaggatg tggcgctggc tatcatcggt
    32461 gccgtgttca aaaacggtta cgtcaaaaac aaagtgatgg agttcgtagg tcccggtgtt
    32521 gcagcgctct ctaccgattt ccgtaacagc gttgacgtga tgaccactga aacgacctgt
    32581 ttaagttctg tctggcaaac cgatgaagaa gtccataact ggctggcgct gcacggtcgc
    32641 ggccaggatt actgccaact taaccctcaa ccaatggcgt actacgatgg ctgcatcagc
    32701 gttgatttaa gcgccatcaa accaatgatt gcgctgccat tccacccgag caacgtgtat
    32761 gaaatcgaca cgctgaacca gaacctgacc gacattctgc gtgagattga aattgagtcc
    32821 gaacgcgtgg cgcacggtaa agccaaactc tcgctgctgg ataaagtgga aaatggtcgc
    32881 ctgaaagtgc agcaggggat tatcgcgggc tgttctggcg gtaactacga aaacgtcatc
    32941 gcggcggcga atgcgctgcg cggtcaatcc tgcggcaacg ataccttctc attggcggtt
    33001 tacccgtcat cacagccggt gtttatggat ctcgccaaaa aaggtgtggt agcagatttg
    33061 attggcgcag gcgcaatcat cagaaccgcg ttctgcggcc catgctttgg tgcgggcgat
    33121 acgccaatca acaacggttt gagtattcgc cacaccacgc gtaacttccc gaaccgcgaa
    33181 ggctctaagc cagctaatgg gcagatgtca gcggtggcgt tgatggacgc tcgttctatc
    33241 gctgcaactg cggcaaacgg tggctattta acctctgcca gcgaactcga ttgctgggac
    33301 aacgtgccgg agtacgcctt cgatgtaacg ccgtataaaa atcgcgtcta tcaaggcttt
    33361 gtgaaagggg cgacccagca accgctgatc tacggaccga acatcaaaga ctggccggaa
    33421 ttgggcgcgc tgactgacaa tatcgtcctc aaagtgtgct cgaagatcct cgacgaagtc
    33481 accaccaccg acgaactgat tccttccggt gaaacctctt cttatcgttc aaatccgatt
    33541 ggtctggcgg agtttaccct gtcacgccgc gatcccggtt atgttggcag aagtaaagcg
    33601 actgctgagc tggaaaatca gcgtctggcg gggaatgtca gcgagctgac agaggtgttt
    33661 gcgcgcatta agcagattgc tggtcaggag catattgatc cgctgcaaac tgaaattggc
    33721 agcatggtct atgcggtgaa accaggcgat ggttctgcgc gtgagcaggc ggcgagctgc
    33781 cagcgtgtga ttggcggtct ggcgaatatt gccgaggaat acgcgactaa acgctaccgt
    33841 tctaacgtca tcaactgggg gatgttaccg ctgcaaatgg cggaagcacc aacgtttgaa
    33901 gtgggggatt acatttacat ccctggcatt aaagcggcgc tggataatcc tggaacgacg
    33961 tttaaaggtt atgtgatcca tgaagatgca ccggtaacgg agattacgct ctatatggaa
    34021 agtctgactg ctgaagagcg cgagattatc aaggcgggta gtttgattaa cttcaataaa
    34081 aaccgtcaga tgtaaaaagc gccatgtgaa tgtaggtcgc atccggcact tattgtcgga
    34141 tgcgatgctg gcgcatctta tccgacctac aaatcgcatc gaaccgtagg ccggataagg
    34201 cgtttacgcc gcatccggca catagttaac agctcctact tcttcgcctc tgcaaccact
    34261 ttgctaccca cgccgcggtt attgtattcc cacatgcggt tgtagttagt gtcattcaga
    34321 ttgcgctgta cttcgtcgtt atcatcaacg ctgccggtgt tacccgcgaa tggacgattg
    34381 gaaatcaccg catcagccca cggtttggct gtgttaaaac cttcgttgat ggcgctatca
    34441 cggatcacca cctgaccgtt ggtattggca tcaacatcca gcgagcggcc cagttgcgcc
    34501 acgccatcac cggaagcatt gaaacgactg tttacggcga ggaaaccgta gtaaatgttg
    34561 gacagcgtag ccggtgcaaa cacatacgct tcttgctgag tacgtgagtt caccacgcgg
    34621 aattcggtat tatcgaacac cactgcgccg cgaccagaaa cgatatccac atccccttca
    34681 atgtagctgt tggtcaccag cgtacgcggc tgacgattcg tttccagacg gttctgcaca
    34741 ccgctgttgg tgacaaagaa ggtgttctga cgaccgagaa tgttaacgtt gttaatctgt
    34801 acctggtcac catcagtacg cagtgccacc gccggatggt tacccgcatc tacgctatcg
    34861 cccagcgtgt tttcgatggt caggttttgc agttgcagtc cattgttttg tgaccagaag
    34921 accgcagagc agagaacgcc gatactgtcg ctgcgtttgc tctggcagct atcgtacata
    34981 taccacgctg gtttacctgg catatatttg ccgcgcgggt tgacgtcgtg acgccagtcg
    35041 gcagggctca tgcccccatc aagggaaagc ccaatcttca catcaatcgg tttttcacct
    35101 gtaccgtaca gagtaattcc acccggagcg gcagggacat ataccgttcc ctgatactca
    35161 ccaggcatca cggcaatata ctggcgcttg ttggtacgct tgataattgc cgcatctacc
    35221 gccgcctgaa tcgtggtatg cgttacacct tgagtacccg ccgggccgac aacaaagtca
    35281 ggttgtgcag gcagggtaat cggggaagga ttccacgctg ccgcacctgg tgtcagggat
    35341 gcaaaatagt gttgagcatc gaaattctgc gcttcttttg ccgacagaat cgggcgagaa
    35401 gaggtaccag gcgcggtttg atcagaagga cgttgatcgg gcggtgttga gctacaggcg
    35461 gtcagcgtca cgccaaaagc caatgccagc gccagacggg aaactgaaaa tgtgttcaca
    35521 ggttgctccg ggctatgaaa tagaaaaatg aatccgttga agcctgcttt tttatactaa
    35581 gttggcatta taaaaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
    35641 caaaataaaa tcattatttg atttcaattt tgtcccactc cctgcctctg tcatcacgat
    35701 actgtgatgc catggtgtcc gacttatgcc cgagaagatg ttgagcaaac ttatcgctta
    35761 tctgcttctc atagagtctt gcagacaaac tgcgcaactc gtgaaaggta ggcggatccc
    35821 cttcgaagga aagacctgat gcttttcgtg cgcgcataaa ataccttgat actgtgccgg
    35881 atgaaagcgg ttcacgacga gtagatgcaa ttatggtttc tccgccaaga atctttttgc
    35941 atttatcaag tgtttccttc attgatatcc cgagagcatc aacatgcaat gttgttggga
    36001 tggcaatttt tacgcctgtt ttgctttgct cgacataaag atatccatct acgatatcag
    36061 accacttcat ttcgcataaa tcaccaactc gctgcccggt aacaacagcc agttccattg
    36121 caagtctaag ccaacatggt gatgattctg ctgcttgata aattttcagg tattcgtcag
    36181 ccgtaagtct tgatctcctt acctctgatt ttgcagcgcg agtggctgcg accgggtttg
    36241 ttgttatatg gccttcagct atagcctctc ggaatgcatc gctcagtgtt gatctgatta
    36301 acttggctga tgccgccttg ccctcgtcta tgtatccatt gagcattgcc gcaatttctt
    36361 ttgtggtgat gtcttcaagt ggagcatcag gcagacccct ccttattgct ttaattttgc
    36421 tcatgtaatt tatgagtgtc ttctgcttga ttcctctgct ggccaggatt ttttcgtagc
    36481 gatcaagcca tgaatgtaac gtaacggaat tatcactgtt gattctcgct gtcagaggct
    36541 tgtgtttgtg tcctgaaaat aactcaatgt tggcctgtat agcttcagtg attgcgattc
    36601 gcctgtctcg gcctaatcca aactctttac ccgtccttgg gtccctgtag cagtaatatc
    36661 cattgtttct tatataaagg ttagggggta aatcccggcg ctcatgactt cgccttcttc
    36721 ccatttctga tcctcttcaa aaggctacct gttactggtc gatttaagtc aacctttacc
    36781 gctgattcgt ggaacagata ctctcttcca tccttaaccg gaggagggaa tatcctgcac
    36841 tcgcgtaccc atcgacgaac tgtttcaagg cttcttgggc gtcgctggcg tgcgttccac
    36901 tcctgaagtg tcaagtacat cgcaaagtct ccgcaattac acgcaagaaa aaaccgccat
    36961 caggcggctt ggtgttcttt cagttcttca attcgaatat tggttacgtc tgcatgtgct
    37021 atctgcgccc acagcatcca gtggtcatag cagtcgctga tgttctcggc ttcgataact
    37081 ctgttgaatg gttctccatt ccattcacct gtgactcgga agtgcattta tcatctccat
    37141 aaaaaaaact cgccgtagcg agttcagata aaagaaatcc ccgcgagtgc gaggatagtt
    37201 attcattgtc gatatttaca tttatttcga atatctttac tgggtaatcg ccaaagtcat
    37261 atatttttta ttctgtttta taagcactac caagtactgc ttattacaaa taagaaaagg
    37321 ctgaaaaaaa tatttaaggc cacaactgtg gccctaaatt tacttacggc actgcgttaa
    37381 tcagattaca aatacctctt ttggcagggt ctgaattcaa cttctgaaaa ttggcgttca
    37441 acatagccgt gtgtaagtga ttctcaacct caaagaatag gtcgattttg ccctctttgg
    37501 ctgcctgttt cttccaaata ttcacttggt catcagcaat acgactagcg caagtgaaca
    37561 agaacgaact gcctctagga atggatgtcg tgctgtaata ctcaccatct acttctacat
    37621 atttagctcg gaatgactct aaggttagct tattgccgtt cttgaccgac ttagcttctg
    37681 ccttacttgt atcttcctgc tctttagaat gtttctgcgc cgacgtgggt atgtcattgg
    37741 ccgctgttac gctgactgaa ctccccaaac ctttgaggcc attaatttgg ataagataag
    37801 ccagctccag agccttatta tcagtgagtt ttccacgcat atttacccac tgacgttgaa
    37861 ccatttttag ggtgtctgcg ttctctgggt ttcctttgag ggcactagaa taggcagagc
    37921 taagctgttc gtctaatttt gacagacgtt cgttatcgca gattttatgt tcgattgctg
    37981 tcgaagcctt ttggcagtca aagctggctg caaatgcacc cggcgatgcc actaacaatg
    38041 atgccagtag gatatttttc atattcactc cataaataca aaagcgcatt actatagcac
    38101 ttaaccgatc gcttatctcg cccgttcgtc attcttatct aaccagaaca tgcgactgcc
    38161 acagcacatg tcgaggattg ctgcatgtcc agtcactggt tgcctccttt gcgaagctgg
    38221 gcggctaact catcacatat gtgcgtcaaa gagcaaagtt tgattgatgg atgttcgcgc
    38281 atcatctcca ccccctgcgc ccgtacttca gccaggaaag catcggtggc tggcatattt
    38341 cctgttgcct tcatggcctc caaaataacc agaacaccat ctctcccaat ctcctcgcag
    38401 ataacctcgg tgctgtcgcc aacaacatcg cagaattcct gaactgcttt acgagccagc
    38461 tcattctccg ccgccagcgc cgaaaacttc tcgtgtgcca acttaacagc tgcatcagcc
    38521 tgcttaattg actcagtcgc tttctggtgg tcttcggcca gccctgctaa atcagcctcc
    38581 agttcggcta ggcgttcatt taatgcatct cgttcatcca gtagagccag cacaacctga
    38641 ggtgtgactt tcatacgaaa tgccagcaat ttttgaggtg ttgctactgt ttcaattgct
    38701 actgccgcct cacgcagtgc ctgagagtta atttcgctca cttcgaacct ctctgtttac
    38761 tgataagttc cagatcctcc tggcaacttg cacaagtccg acaaccctga acggccaagc
    38821 gtcttcgttc atctatcgga tcgccacact cacaacaatg agtggcagat atagcctggt
    38881 ggttcaggcg gcgcattttt attgctgtgt tgcgctgtaa ttcttcaatt tctgatgctg
    38941 aatcaatgat gtctgccatc tttcattaat ccctgaattg ttggttaata cgcttgaggg
    39001 tgaatgcgaa taataaaaaa ggagcctgta gctccctgat gattttgctt ttcatgttca
    39061 ccgttcctta aagacgccgt tcaacatgcc gatcgccagg cttaaatgag tcggtgtgaa
    39121 tcccatcagc gttaccgttt cgcggtgctt ctttagtacg ctacggcaaa tgtcatcgac
    39181 gtttttatcc ggaaactgct gtctggcttt tttgatttca gaattagcct gacgggcaat
    39241 gctgcgaagg gcgttttctt gctgaggtgt cattgaacaa gccccatgtc ggcaagcata
    39301 agcacacaga atatgaagcc cgctgccaga aaaatgcatt cagtggttgt catacctggt
    39361 ctctctcatc tgcttctgct ttcgccacca tcatttccag cttttgtgaa agggatgcgg
    39421 ctaacgtatg aaattcttcg tctgtttcta ctggtattgg cacaaacctg actccaattt
    39481 gagcgaggct atgtgccatc tcgatactcg ttcttaactc aacgggagat gctttgtgca
    39541 tacagctccc cgtttattat ttatctcctc agccagccgc tgtgctttca ggggatttct
    39601 gataacagaa aggccgggaa atacccagcc tcgctttgta atggagtaga cgaaagtgat
    39661 cgcgcctacc cggatattat cgtgaggatg cgtcatcgcc attgctcccc aaatacaaaa
    39721 ccaatttcag ccagtgcctc gtccattttt tcgatgaact ccggcaccat ctcgtcaaaa
    39781 ctcgccatgt aattttcatc ccgctcaacc acgacataat gcaggccttc acgcttcatt
    39841 cgtgggtcat agttggcaaa gtaccaggca tcttttcgcg tcacccacat gctgtactgc
    39901 acctgggcca tgtaagccga ttttatggcc tcgaaaccac cgagccggaa cttcatgaaa
    39961 tcccgggagg taaacgggca tttcagttca aggccgttgc cgtcactgca taaaccatcg
    40021 ggagagcagg cggtgcgcat actttcgtcg cgatagatga tcggggattc agtaacatta
    40081 acgccggaag tgaactcaaa gagggttctg gcgtcgttct cgtactgttt tccccaggcc
    40141 agcgccttag cgttaacttc cggagccaca ccggtgcaaa cctcagccag cagggtgtgg
    40201 aagtaggaca ttttcatgtc aggccacttc tttcctgagc ggggctttgc tatcacgttg
    40261 tgaacttctg aagcggtgat gacgccgagc cgtaatttgt gccatgcatc atccccctgt
    40321 tcgacagctc tcacgtcgat cccggtacgc tgcaggataa tgtccggtgt catgcagcca
    40381 ccttctgttc agaggctttc tgtttcagga atccaagagc tttcattgct tcgacctgtg
    40441 tcagttctga cgatgcgcga atgtcgcggc gaaatatctg ggaacagagc ggcaataagt
    40501 cgtcatccca tgttttgtcc agggcaatca gcatagtgtt aatctcctgc atggtttcat
    40561 cgttaaccgg agtgatgtcg cgttccggct gacgttctgc agtgtatgcg gtattttcga
    40621 caatgcgctc ggcttcatcc ttgtcataga taccagcaaa tccgaaggcc agacgggcac
    40681 actgaatcat ggctttatgc cgtaacatcc gtttgggatg cgactgccac ggtccggtga
    40741 tttctctgcc ttcgcgggtt ttgaatggtt cgcggcggca ttcatccatc cactcggtaa
    40801 cgcagatcgg atgattacgg tccttgcggt aaatccggca tgtacatgat tcattgtcct
    40861 gctcaaagtc catgccatca aactgctggt tttcattgat gatacgggac cagccatcaa
    40921 cgcccaccac cggaacgatg ccgttctgct tgtcagggaa ggcgtaaatt tctttcgtcc
    40981 acggattaag gccgtactgg ttggcgacga tcaacaatgc gatgaactgc gcatcgctgg
    41041 catcgccttt aaatgccgtc tggcgaagag tggtgatcag ttcctgtggg tcgacagaat
    41101 ccatgccgac acgttcagcc agcttccctg ccagcgttgc gagtgctgta ctcatccgtt
    41161 ttatacctct gaatcaatat caacctgatg gtgagcaatg gtttcaacca tgtaccggat
    41221 gtgttctgcc atgcgctcct gaaactcaac atcgtcatca aacgcacggg taatggcttt
    41281 tttgctggcc ccgtggcgtt gcaaatgatc gatgcatagc gattcaaaca ggtgctgggg
    41341 caggcctttt tccatgtcgt ctgccagttc tgcctctttc tcttcacggg ctatctgctg
    41401 gtagtgacgc gcccagctct gagcctcaag acgatcctga atgtaataag cgttcatggc
    41461 cgaactcctg aaatagctgt gaaaatatcg cccgcgaaat gccgggctga ttaggaaaac
    41521 aggaaagggg gttagtgaat gcttttgctt gatctcagtt tcagtattaa tatccatttt
    41581 ttataagcgt cgacggcctc acgaaacatc ttttcatcgc caataaaagt ggcaatagtg
    41641 aattttgtct ggatagccat aagtttttta tccatttttg ggtactcctg gctgattaag
    41701 tatgtcgata aggcgtttcc atccgtcacg taatttacgg gtgattcgtt caagtaaaga
    41761 ttcggaaggg cagccagcaa caggccaccc tgcaatggca tattgcatgg tgtgctcctt
    41821 atttatacat aacgaaaaac gcctcgagtg aagcgttatt ggtatgcggt aaagccgcac
    41881 tcaggcggcc ttgatagcca tatcatctga atcaaatatt cctgatgtat cgatatcggt
    41941 aattcttatt ccttcgctac catccattgg aggccatcct tcctgaccat ttccatcatt
    42001 ccagtcgaac tcacacacaa caccatatgc atttaagtcg cttgaaattg ctataagcag
    42061 agcatgttgc gccagcatga ttaatacagc atttaataca gagccgtgtt tattgagtcg
    42121 gtattcagag tctgaccaga aattattaat ctggtgaagt ttttcctctg tcattacgtc
    42181 atggtcgatt tcaatttcta ttgatgcttt ccagtcgtaa tcaatgatgt attttttgat
    42241 gtttgacacc tattcatatc ctcatagata aaaaatcgcc ctcacactgg agggcaaaga
    42301 agatttccaa taatcagaac aagtcggctc ctgtttagtt acgagcgaca ttgctccgtg
    42361 tattcactcg ttggaataaa tacacagtgc agtgtttatt agtatgcctg tcttttaacc
    42421 acatcaggct cggtggttct cgtgtacccc tacagcgaga aaaatagtaa aatcctctta
    42481 cccctacagt aagagagtga tttatatgtc tgatgaaaaa ggaataataa gtaggattac
    42541 agatgcagtt tctggtgccg gaggtgctct gatgagtgct gttggcgcag taaaggaaat
    42601 ccagaaaatg caaatagact actcagtaaa agaaaaaaca tacgaacttg tcgataagct
    42661 catggatgcc caacagcagc aaatgtcact taatgagctt ttgatgattt ctaaggacaa
    42721 gattattgag cttgaagaaa agataaatcg agcctctaag tgggaagagg aaaagaaaaa
    42781 ctatgagatg tatacaccta ctgttgctac ggtggtttat cgtctcaaaa aatcggcaaa
    42841 taccggtcag ccaatgcact atctttgcgc tcaatgctat gaatcttctg tgaaatccat
    42901 acttcaatac gaaggatttg caccaccttc taaccatagg atgagatgcc ataggtgtaa
    42961 cgcctcctat ctgttcccta aatctgcatt ttctaaataa gtgatataga tttaccttta
    43021 attttctggc gtcctctgca tgttatgccg cgttcgccag gcttgctgta ccatgtgcgc
    43081 tgattcttgc gctcaatacg ttgcaggttg ctttcaatct gttcgtggta ttcagccagc
    43141 accgtaaggt ctatcggatt cagtgcgctt tctactcgtg atttcggttt gcgattcagc
    43201 gagagaatag ggcggttaac tggttttgcg cttaccccaa ccaacagggg atttgctgct
    43261 ttccattgag cctgtttctc tgcgcgacgt tcgcggcggc gtgtttgtgc atccatctgg
    43321 attctcttgt cagttagctt tggtggtgtg tgggagtcgt agtcctgaac gaaaacaccc
    43381 cacgattggc tcatttgcag ctaatccgga ttcgcacttc cggccaatgt ttcgtttcgt
    43441 atcacacacc ccaaagcctt ctgctttgaa tgctgccctt cttcagggct taatttttaa
    43501 gagcatcacc ttcatgtggg ttagtgcgtc ctgctggtgt gtttagtatc accgctagtg
    43561 gtatttgtgt caacaccgcc agagataatt tatcaccgca gatggttatc tgtatgtttt
    43621 ttatatagat ttattttttg cagggttgtg tggcttggga ggtgatcgag agatcagaat
    43681 tgcgttgttt agtgagttgc acctattaat tttaaaataa atacaattgg ttatgtgttt
    43741 ttaggtgggg cgatcgtgag gcaaagaaaa cccggctcgg tggccgggtt gatgggatta
    43801 tcgtttattt ctttgatgtt tctttgcaga ctcagcttga gtgtttccat acctgcgttt
    43861 accgctattg ttttttcccc taggcttgcc tttttttcga tcttcccacc agtcaggtgc
    43921 cgggtctggc tcaacatgta aagcatcggc aagtcctgat atcaagctgt atgactgaac
    43981 tccatcaatg tgctctgaca gtgatggggt aacattctgt cttagtggat caagaataaa
    44041 atcaatacct ttaattctgg cgtgtttggc ggcaggcaca aagtctgagt ccccagcaac
    44101 aaggacaatt acatcaacaa gtttttcata agctaaagtc gtgatatcca tccctagctt
    44161 gatatcaact tgtttttgtt tgatgtcata gtaaaaatca tcatttgtca gttcatccca
    44221 ttttttcgtt cctttcatca gagcatcaag ggagaatgta gttagttgcc atcgcttatt
    44281 atcaacaaga ttccctaatc ttaaggctgt ttttcgagtt tttcttaact cttcatgcag
    44341 ctccgttctc agaatatatg atttttcgag tttgaaattc ttgcgaccag gcgtcttatt
    44401 gccaggctca ggaagtggaa ggcgtgtttg aatgtcgagt ggtggacagt catagaagta
    44461 aattctataa agctcaagcg gttccctacg ttcttgtgat tgacgttttc cattaagatg
    44521 ggaaagaacc attgaccata ttactttcat tatgcattgc gcagtcagtt catgctcggc
    44581 gaagtgttta cgatgcgtag catgaacacg ctgcatgaaa aaacccgcat caattaaaat
    44641 tgctgctttc ttcataagag tcccaaaaaa atagcccaga gccgttatgc agatattaac
    44701 aattgtctgc gaacggggct gggcttggtt taattaatct atgccaagtt gactgtgccg
    44761 tcaacataaa acttacttcc tatcacccaa acgtctcttc aggccactga ctagcgataa
    44821 ctttccccac aacggaacaa ctctcattgc atgggatcat tgggtattgt gggtttagtg
    44881 gctgtagaaa cacctgaccg ctatccctga tcagtttctt gaaggtaaac tcatcaccac
    44941 caagtctggc tatacagaaa tcgcctggct caacagcctg ctcagggtca accagaatta
    45001 acatcccgtc aggaaagctg ggcttggatc ctgttggtgc ggtcatggaa ttaccttcaa
    45061 cctcaagcca gaatgcagag ccactggctt ttttggttgt gcttacccat ttctccgcat
    45121 cgcctttggt aaaggttcta agctcaggcg agaacatccc ggcctgaaca tgagaaaaaa
    45181 cagggtactc atactcactt ctaagtgacg gctgcatact aaccgcttca tacatctcgt
    45241 agatttctct ggcgattgaa gggctaaatt cttcaacgct aacgttgaga atttttgcaa
    45301 gcaatgcggc gttataagca tttaatgcat tgatgccatt aaataaagca ccaacgcctg
    45361 actgccccat ccccatcttg tctgcgacag attcctggga taagccaagt tcatttttct
    45421 ttttttcata aatagcttta aggcgacgtg cgtcctcaag ctgctcttgt gttaatggtt
    45481 tcttttttgc gctcatacgt taaatctatc accgcaaggg ataaatatct aacaccgtgc
    45541 gtgttgacta ttttacctct ggcggtgata atagttgcat gtactaagga ggttgtatgg
    45601 aacaacgcat aaccctgaaa gattatgcaa tgcgctttgg gcaaaccaag acagctaaag
    45661 atctcggcgt atatcaaagc gcgatcaaca aggccattca tgcaggccga aagatttttt
    45721 taactataaa cgctgatgga agcgtttatg cggaagaggt aaagcccttc ccgagtaaca
    45781 aaaaaacaac agcataaata accccgctct tacccatccc cgccctgaaa aagggcatcc
    45841 aattaaacca cacctatggt gtatgcattt atttgcatac attcaatcaa ttgttatcta
    45901 aggaaatact tacatatggt tcgtgcaaac aaacgcaacg aggcactacg aatcgagagt
    45961 gcgttgctta acaaaatcgc aatgcttgga actgagaaga cagcggaagc tgtgggagtt
    46021 gataagtcgc agatcagcag gtggaagaga gactggattc ccaagttctc aatgctgctt
    46081 gctgttcttg aatggggggt cgttgacgac gacatggctc gattggcgcg acaagttgct
    46141 gcgattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg
    46201 gagttctgag gtcattactg gatctatcaa caggagtcat tatgacaaat acagcaaaaa
    46261 tactcaactt cggcagaggt aactttgccg gacaggagcg taatgtggca gatctcgatg
    46321 atggttacgc cagactatca aatatgctgc ttgaggctta ttcgggcgca gatctgacca
    46381 agcgacagtt taaagtgctg cttgccattc tgcgtaaaac ctatgggtgg aataaaccaa
    46441 tggacagaat caccgattct caacttagcg agattacaaa gttacctgtc aaacggtgca
    46501 atgaagccaa gttagaactc gtcagaatga atattatcaa gcagcaaggc ggcatgtttg
    46561 gaccaaataa aaacatctca gaatggtgta tccctcaaaa cgagggacaa tcccctaaaa
    46621 cgagggataa aacatccctc aaattggggg attgctatcc ctcaaaacag ggggacacaa
    46681 aagacactat tacaaaagaa aaaagaaaag attattcgtc agagaattct ggcgaatcct
    46741 ctgaccagcc agaaaacgat ctttctgtgg ttaaaccgga tgctgcaatt cagagcggca
    46801 gcaagtgggg gacagcagaa gacctgaccg ccgcagagtg gatgtttgac atggtgaaga
    46861 ctatcgcacc atcagccaga aaaccgaatt ttgctgggtg ggctaacgat atccgcctga
    46921 tgcgtgaacg tgacggacgt aaccaccgcg atatgtgtgt gcttttccgc tgggcctgcc
    46981 aggacaactt ctggtccggt aacgtgctga gtccggccaa actccgcgac aagtggaccc
    47041 agctcgaaat caaccgtaac aagcaacagg caggcgtgac agccagcaaa ccaaaactcg
    47101 acctgacaaa cactgactgg atttacgggg tggatttatg aaaaacatcg ccgcacagat
    47161 ggttaacttt gaccgtgagc agatgcgtcg gatcgccaac aacatgccgg aacagtacga
    47221 cgaaaagccg caggtacaac aggtagcgca gatcatcaat ggtgtgttca gccagttact
    47281 ggcaactttc ccggcgagcc tggctaaccg ggaccagaac gaactgaacg aaatccgccg
    47341 ccagtgggtg ctggctttcc gggaaaacgg gatcaccaca atggaacagg ttaacgcagg
    47401 aatgcgcgta gcccgtcggc agaatcgacc atttctgcca tcacccgggc agtttgttgc
    47461 atggtgccgg gaagaagcat ccgttatcgc cggactgcca aacgtcagcg agctggttga
    47521 tatggtttac gagtattgcc ggaagcgagg cctgtatccg gatgcggagt cttatccgtg
    47581 gaaatcaaac gcgcactact ggctggttac caacctgtat cagaacatgc gggccaatgc
    47641 gcttactgat gcggaattac gccgtaaggc cgcagatgag cttgtccata tgactgcgag
    47701 aattaaccgt ggtgaggcga tccctgaacc agtaaaacaa cttcctgtca tgggcggtag
    47761 acctctaaat cgtgcacagg ctctggcgaa gatcgcagaa atcaaagcta agttcggact
    47821 gaaaggagca agtgtatgac gggcaaagag gcaattattc attacctggg gacgcataat
    47881 agcttctgtg cgccggacgt tgccgcgcta acaggcgcaa cagtaaccag cataaatcag
    47941 gccgcggcta aaatggcacg ggcaggtctt ctggttatcg aaggtaaggt ctggcgaacg
    48001 gtgtattacc ggtttgctac caaggaagaa cgggaaggaa agatgagcac gaacctgatt
    48061 tttaaggagt gtcgccagag tgccgcgatg aaacgggtat tggcggtata tggagttaaa
    48121 agatgaccat ctacatcact gagctaataa caggcctgct ggtaatcgca ggccttttta
    48181 tttgggggag agggaagtca tgaaaaaact aacctttgaa attcgatctc cagcacatca
    48241 gcaaaacgct attcacgcag tacagcaaat ccttccagac ccaaccaaac caatcgtagt
    48301 aaccattcag gaacgcaacc gcagcttaga ccaaaacagg aagctatggg cctgcttagg
    48361 tgacgtctct cgtcaggttg aatggcatgg tcgctggctg gatgcagaaa gctggaagtg
    48421 tgtgtttacc gcagcattaa agcagcagga tgttgttcct aaccttgccg ggaatggctt
    48481 tgtggtaata ggccagtcaa ccagcaggat gcgtgtaggc gaatttgcgg agctattaga
    48541 gcttatacag gcattcggta cagagcgtgg cgttaagtgg tcagacgaag cgagactggc
    48601 tctggagtgg aaagcgagat ggggagatcg ggctgcatga ctatcaaatc aaatacgcca
    48661 gcacacgaca aggactgctg gcaaacgccg ctttggcttt ttgatgcact ggatattgag
    48721 tttggattct ggctggattc tgcagcgagc gacaaaaatg ctctgtgtgc tcactggcta
    48781 actgaggccg acgacgcgct caattctgag tgggtaagcc acggtgcaat ctggaataac
    48841 ccaccgtaca gcaatatcag gccgtgggtg gaaaaagccg ctgagcagtg catacaacag
    48901 cgacagacgg tagttatgct tgtgccagag gatatgtcag tcggatggtt cagcaaggct
    48961 ctggagagtg tcgacgaagt tcgcattatc actgatggac ggattaattt tatcgaacca
    49021 tcgacagggc tggagaagaa gggaaacagc aaaggctcca tgctgctgat ttggcgaccg
    49081 ttcatcagtc ctcgacggat gtttactacc gtatccaaag cggcattgat ggcgatcggg
    49141 cagggcgtca ggagggcggc atgag
//
LOCUS       DAPHCV010000021        48478 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.021, whole genome shotgun sequence.
ACCESSION   DAPHCV010000021 DAPHCV010000000
VERSION     DAPHCV010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.021"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            155..1372
                     /gene="alaA"
                     /locus_tag="RH033_001234"
     CDS             155..1372
                     /gene="alaA"
                     /locus_tag="RH033_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HDV2990449.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1456..2055
                     /gene="yfbR"
                     /locus_tag="RH033_001235"
     CDS             1456..2055
                     /gene="yfbR"
                     /locus_tag="RH033_001235"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HDV2990450.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2114..3946)
                     /locus_tag="RH033_001236"
     CDS             complement(2114..3946)
                     /locus_tag="RH033_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDV2990451.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGEAMEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4033..4683)
                     /gene="hxpA"
                     /locus_tag="RH033_001237"
     CDS             complement(4033..4683)
                     /gene="hxpA"
                     /locus_tag="RH033_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HDV2990452.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4694..5188)
                     /locus_tag="RH033_001238"
     CDS             complement(4694..5188)
                     /locus_tag="RH033_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HDV2990453.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5272..5727)
                     /locus_tag="RH033_001239"
     CDS             complement(5272..5727)
                     /locus_tag="RH033_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HDV2990454.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6065..7267
                     /gene="ackA"
                     /locus_tag="RH033_001240"
     CDS             6065..7267
                     /gene="ackA"
                     /locus_tag="RH033_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDV2990455.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7342..9486
                     /gene="pta"
                     /locus_tag="RH033_001241"
     CDS             7342..9486
                     /gene="pta"
                     /locus_tag="RH033_001241"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDV2990456.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9676..11196
                     /gene="yfcC"
                     /locus_tag="RH033_001242"
     CDS             9676..11196
                     /gene="yfcC"
                     /locus_tag="RH033_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDV2990457.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11229..11771)
                     /gene="yfcD"
                     /locus_tag="RH033_001243"
     CDS             complement(11229..11771)
                     /gene="yfcD"
                     /locus_tag="RH033_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HDV2990458.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11829..12380)
                     /gene="yfcE"
                     /locus_tag="RH033_001244"
     CDS             complement(11829..12380)
                     /gene="yfcE"
                     /locus_tag="RH033_001244"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HDV2990459.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12436..13080)
                     /gene="yfcF"
                     /locus_tag="RH033_001245"
     CDS             complement(12436..13080)
                     /gene="yfcF"
                     /locus_tag="RH033_001245"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HDV2990460.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERSTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13216..13863
                     /gene="yfcG"
                     /locus_tag="RH033_001246"
     CDS             13216..13863
                     /gene="yfcG"
                     /locus_tag="RH033_001246"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HDV2990461.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     HISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAASQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQHGDERSDS"
     gene            13920..14282
                     /gene="folX"
                     /locus_tag="RH033_001247"
     CDS             13920..14282
                     /gene="folX"
                     /locus_tag="RH033_001247"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HDV2990462.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14303..15196
                     /locus_tag="RH033_001248"
     CDS             14303..15196
                     /locus_tag="RH033_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HDV2990463.1"
                     /translation="MKIVITGGTGLIGRHLIPRLLELGHQITVMTRNPQKASSVLGPR
                     VALWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCAHWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15244..16146)
                     /gene="rpnB"
                     /locus_tag="RH033_001249"
     CDS             complement(15244..16146)
                     /gene="rpnB"
                     /locus_tag="RH033_001249"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HDV2990464.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGAGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGRNDGLILGKREEALRIAQEMLE
                     RGLDRELVMMVTRLSPDDLIAQSH"
     gene            complement(16343..17116)
                     /gene="hisP"
                     /locus_tag="RH033_001250"
     CDS             complement(16343..17116)
                     /gene="hisP"
                     /locus_tag="RH033_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HDV2990465.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17124..17840)
                     /locus_tag="RH033_001251"
     CDS             complement(17124..17840)
                     /locus_tag="RH033_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2990466.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17837..18523)
                     /gene="hisQ"
                     /locus_tag="RH033_001252"
     CDS             complement(17837..18523)
                     /gene="hisQ"
                     /locus_tag="RH033_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HDV2990467.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVLKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18613..19395)
                     /gene="hisJ"
                     /locus_tag="RH033_001253"
     CDS             complement(18613..19395)
                     /gene="hisJ"
                     /locus_tag="RH033_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HDV2990468.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19616..20398)
                     /gene="argT"
                     /locus_tag="RH033_001254"
     CDS             complement(19616..20398)
                     /gene="argT"
                     /locus_tag="RH033_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HDV2990469.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20664..21233)
                     /gene="ubiX"
                     /locus_tag="RH033_001255"
     CDS             complement(20664..21233)
                     /gene="ubiX"
                     /locus_tag="RH033_001255"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HDV2990470.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21328..22845)
                     /gene="purF"
                     /locus_tag="RH033_001256"
     CDS             complement(21328..22845)
                     /gene="purF"
                     /locus_tag="RH033_001256"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDV2990471.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(22882..23370)
                     /gene="cvpA"
                     /locus_tag="RH033_001257"
     CDS             complement(22882..23370)
                     /gene="cvpA"
                     /locus_tag="RH033_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HDV2990472.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23629..24279)
                     /gene="dedD"
                     /locus_tag="RH033_001258"
     CDS             complement(23629..24279)
                     /gene="dedD"
                     /locus_tag="RH033_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HDV2990473.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAVNNTEFEPEPA
                     PVAPPKPKPVESPKPKVEAPPASKPVVEEKAAPTGKAYVVQLGALKNADKVNEIVGKL
                     RGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYTPN"
     gene            complement(24269..25537)
                     /gene="folC"
                     /locus_tag="RH033_001259"
     CDS             complement(24269..25537)
                     /gene="folC"
                     /locus_tag="RH033_001259"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDV2990474.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKSA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25607..26521)
                     /gene="accD"
                     /locus_tag="RH033_001260"
     CDS             complement(25607..26521)
                     /gene="accD"
                     /locus_tag="RH033_001260"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HDV2990475.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPLVPDQEPEA"
     gene            complement(26677..27336)
                     /locus_tag="RH033_001261"
     CDS             complement(26677..27336)
                     /locus_tag="RH033_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV2990476.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAFN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27419..28231)
                     /gene="truA"
                     /locus_tag="RH033_001262"
     CDS             complement(27419..28231)
                     /gene="truA"
                     /locus_tag="RH033_001262"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDV2990477.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSRYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28231..29244)
                     /locus_tag="RH033_001263"
     CDS             complement(28231..29244)
                     /locus_tag="RH033_001263"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDV2990478.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29310..30446)
                     /gene="pdxB"
                     /locus_tag="RH033_001264"
     CDS             complement(29310..30446)
                     /gene="pdxB"
                     /locus_tag="RH033_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HDV2990479.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDDTWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEALGITTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTRHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30545..31540
                     /gene="flk"
                     /locus_tag="RH033_001265"
     CDS             30545..31540
                     /gene="flk"
                     /locus_tag="RH033_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HDV2990480.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31537..32715)
                     /locus_tag="RH033_001266"
     CDS             complement(31537..32715)
                     /locus_tag="RH033_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2990481.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVPAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSH
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(32980..34200)
                     /gene="fabB"
                     /locus_tag="RH033_001267"
     CDS             complement(32980..34200)
                     /gene="fabB"
                     /locus_tag="RH033_001267"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HDV2990482.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34359..36365
                     /gene="mnmC"
                     /locus_tag="RH033_001268"
     CDS             34359..36365
                     /gene="mnmC"
                     /locus_tag="RH033_001268"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HDV2990483.1"
                     /translation="MKHYAIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHLHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGHVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGSTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RLYDLLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPTELAVAVGANEVEGVTGVV
                     TNCGGITYPQGGWLCPAELTCNVLKLAQQQGLQIRYQHQLQDLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSEKEARCGV
                     RCATRDHLPMVGNVPDYDVTLVEYASLAEQKDEAVSAPIYDDLFMLAALGSRGLCSAP
                     LCAEILAAQMSNEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36486..36764)
                     /locus_tag="RH033_001269"
     CDS             complement(36486..36764)
                     /locus_tag="RH033_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HDV2990484.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWEHLFQQASQQ"
     gene            complement(36798..37346)
                     /gene="epmC"
                     /locus_tag="RH033_001270"
     CDS             complement(36798..37346)
                     /gene="epmC"
                     /locus_tag="RH033_001270"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HDV2990485.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARREQVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37346..38155)
                     /locus_tag="RH033_001271"
     CDS             complement(37346..38155)
                     /locus_tag="RH033_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDV2990486.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALVAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38155..38979)
                     /gene="mepA"
                     /locus_tag="RH033_001272"
     CDS             complement(38155..38979)
                     /gene="mepA"
                     /locus_tag="RH033_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HDV2990487.1"
                     /translation="MNKTAIVLLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSRQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(38982..40067)
                     /gene="aroC"
                     /locus_tag="RH033_001273"
     CDS             complement(38982..40067)
                     /gene="aroC"
                     /locus_tag="RH033_001273"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDV2990488.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40102..41034)
                     /gene="prmB"
                     /locus_tag="RH033_001274"
     CDS             complement(40102..41034)
                     /gene="prmB"
                     /locus_tag="RH033_001274"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDV2990489.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            41200..41751
                     /gene="smrB"
                     /locus_tag="RH033_001275"
     CDS             41200..41751
                     /gene="smrB"
                     /locus_tag="RH033_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HDV2990490.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(41867..42709)
                     /locus_tag="RH033_001276"
     CDS             complement(41867..42709)
                     /locus_tag="RH033_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HDV2990491.1"
                     /translation="MKKWTMIFASLVLLVMSASVPKSYAGDKLTSASFDSVKIYYGKG
                     KMSIHNTGGLYVTVLTPNEAAYGVYNDAIRKNEQASVISWTGSGPAPVLILTDYGNAD
                     SSNCPGINTATFQCAYETFKITVQSDSYGCPWLASFYTITDQPGFGRYTGPTNHATVC
                     PTIPVASYDISWSENYVSHNKALQLQSNGSTITTTLSTYLMEDGKLCDGSVFDSRGAY
                     CRVVSELLTFTSYGCDKSTVTVSPTRHPVTDKQLHDIVVNVNTSSRQPIDSTCRFQYV
                     LNEL"
     gene            complement(42711..43235)
                     /locus_tag="RH033_001277"
     CDS             complement(42711..43235)
                     /locus_tag="RH033_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2990492.1"
                     /translation="MKKKRALVFICSLMLLYSVTAPAVDNLHFTGNLLGKSCTPVING
                     SLLAEVHFPTIAASDLMNLGQSERVPLVFQLKDCKSSTLFSVRVTLTGTEDSELPGFL
                     AFDASSSASGAGIGIETAAGAAVPVNSTTGVTFPLNQGNNSLNFNAWLQAKGGRDVTS
                     GDFSATATATFEYF"
     gene            complement(43232..43702)
                     /locus_tag="RH033_001278"
     CDS             complement(43232..43702)
                     /locus_tag="RH033_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2990493.1"
                     /translation="MKRISLLMLWGFSSMALSNVSFHGYLVQPPNCTISNGKTIEITF
                     QDVFVDDINGSNYEQTVPYSITCDTAVRDPSMEMKLSWSGTQSDFDDAAVSTDISGLA
                     IRLKQAGQSFKLNTALVVNETALPVLTAVPVKKSGVDLPEADFEAWATLQVDYQ"
     gene            complement(43699..44247)
                     /locus_tag="RH033_001279"
     CDS             complement(43699..44247)
                     /locus_tag="RH033_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2990494.1"
                     /translation="MKSRAAKVNDGEVSMKRSCSVWAGTWLLMCCIGSDFAVAGSKSV
                     SLSLRVLVDAPPPCTVNGATVEFGNVFINKIDGVGYKQPIDYSLVCNNLAMDDLRLQM
                     RATTVVINGETVISTGISGFGIRVQKSSDHSTLDLTSGSWLPFNFSSGVPALEAVPVK
                     QSGTTLSAAEFNASATIVVDYQ"
     gene            complement(44222..44971)
                     /locus_tag="RH033_001280"
     CDS             complement(44222..44971)
                     /locus_tag="RH033_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDV2990495.1"
                     /translation="MTKHLSVKTLATVLSLLAATVSFSALASVTPDRTRLIFNESDKS
                     ISVTLRNNDPKQPYLAQSWIEDEKGVKISSPLAVLPPVQRIDAMMNGQVKIQGLPDIH
                     KLPADRESIFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALEKASMQNPWQHKVT
                     LTRNGQEFTVNNPTPYYVIFSNASAQKNGNPADGFSPIVMSPKTSAPLKVKMASVPVL
                     TYVNDYGARMPLFFTCSGNSCQVDEEQSRKG"
     gene            complement(44991..47630)
                     /locus_tag="RH033_001281"
     CDS             complement(44991..47630)
                     /locus_tag="RH033_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV2990496.1"
                     /translation="MPEHSFLRLRGLSWWIALAISGSPFNVLADDTIQFDARFLELKG
                     NTKIDLGRFSQKGYVEPGKYNLRVHVNNHPLPDEYDIYWYVAENDPNKSYACLPPELI
                     AQFGFKDDFAKSLQWGHDGQCLKTDQIGGMEIKGDLSQSALLVSVPQAYLEYTDDDWD
                     PPSRWDEGIPGLIADYSINAQTRHENGGDDTNDISGNGTVGVNVGPWRLRADWQSDYQ
                     HTRSNDDGDTDDSGTQKNWEWSRYYAWRALPSLKAKLSLGEDYLNSDIFDGFSYIGGS
                     ISTDDQMLPPNMRGYAPDISGVAHTTAKVTVTQMGRVIYETQVPAGPFRIQDIGDSVS
                     GTLHVRIEEQNGQVQEYDVSTASMPFLTRPGQVRYKVTMGRPQNWDHQVAGSFFSGGE
                     ASWGIANGWSLYGGALADENYQSAALGLGRDLALFGALAFDVTHSRVQLDDNSVYGNK
                     TLDGNSYRVSYAKDFDELNSRVTFAGYRFSEKNYMTMSEYLDANDTDRARTGNDKEMY
                     TVTYNQNFRDARVSVYLNYSHHTYWDREDQTNYNMMLSHYFNLGSLRNLSVSLTGYRY
                     EYDKSADKGVYLSLSLPWGDNSTISYNGNYGSGADSSQASLYHRIDDASHYTLSAGTS
                     ENHTSLDGYYSHDGTLAKVDLSANYHEGQYTSAGVSLQGGVTLTAHGGALHRTQNMGG
                     TRLLIDADGVANVPVEGNGSAVYTNMFGKAVVADVNDYYRNQAYIDLNKLPENAEATQ
                     SVVQATLTEGAIGYRKFAVISGEKAMVVLRLQDGSHPPFGAEVKNDNQQQVGLVDDEG
                     NVYLAGVKPGEHMTVFWEGESHCDISLPDPLPNDLFNGLLLPCQQKGGSAPVITHDIQ
                     PVIQEQTQQVTPMEPPMSVSSNQ"
     gene            complement(47716..48282)
                     /locus_tag="RH033_001282"
     CDS             complement(47716..48282)
                     /locus_tag="RH033_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2990497.1"
                     /translation="MSKFVKAAVATAMLMGALTSASVVAAGNNGTVRFYGTIEDSPCS
                     IVPDDHKLEVDMGSIGTGSLTGGKTTTPKDFQIRLQDCNFNTETTMTTTFTGNPYSGN
                     SKNYSLSNMDNGTEIPNVSLVIGDQRGTGYDLGAGIDQDIAMDSSTGKGKQKQTLNFK
                     AWLVGETAAVTPTPAPFETLTTFQITYL"
BASE COUNT        11961 a        13050 c        11991 g        11476 t
ORIGIN      
        1 tgccattatt aagttattaa cagcacaatc aagccttcca ctctggcaaa atcttattct
       61 gcagaccttc aaaacgccgt cctgggggag tacattgttc taagctgact tccacggcag
      121 ggagtggcga taacagcaaa aaaggtcaag attcatgtcc cccattgaaa aatccagcaa
      181 attagagaat gtctgttacg acatccgtgg tccggtgctg aaagaagcaa aacgcctgga
      241 agaagaaggt aacaaggtac tgaaactgaa catcggcaac cctgccccgt tcggttttga
      301 cgcgccagat gaaatcctcg ttgacgtgat tcgcaacctg cctaccgctc aagggtattg
      361 cgattccaaa gggctttact ccgcgcgtaa agccatcatg cagcactacc aggctcgtgg
      421 catgcgtgat gttaccgtgg aagatattta catcggcaat ggtgtatcgg agcttatcgt
      481 tcaggcaatg caggcattgc tgaacagcgg ggacgaaatg ttggttcctg cacctgatta
      541 cccactatgg accgcggcgg tttcgctctc cagcggtaaa gcggtgcatt atctttgcga
      601 tgaatcctct gactggttcc cggaccttga tgatattcgc gctaaaatta cgcctcgtac
      661 gcgtgggatc gttattatca acccaaataa cccaaccggc gcggtatatt ccaaagagct
      721 tttaatggag atagtggaga ttgcacgtca gcataatctc attatcttcg ccgatgaaat
      781 ttatgacaaa attctctacg acgacgctga gcatcactca attgcgccgc tggcacctga
      841 tctgctgacc attaccttta acggactgtc gaaaacgtac cgcgttgctg gcttccgtca
      901 ggggtggatg gtgttgaacg ggccgaaaaa acacgccaaa ggttacatcg aaggtctgga
      961 aatgctggct tcaatgcgcc tgtgtgctaa cgttcctgcg caacacgcca ttcagaccgc
     1021 gctgggcggt tatcagagca tcagtgaatt tattacccct ggcggtcgtc tttatgagca
     1081 gcgtaaccgc gcatgggaat tgatcaatga tattccgggc gtttcctgcg tgaaaccgcg
     1141 cggtgcgctg tacatgttcc cgaaaatcga cgccaaacgc tttaatattc acgacgatca
     1201 gaaaatggtg ctggatttcc tgttgcagga aaaagttctg ttggtgcaag ggacggcatt
     1261 caactggccg tggccggatc acttccgcat tgtcacgcta ccgcgtgtcg atgatatcga
     1321 gctgtctttg agcaagttcg cgcgtttcct ttctggttat catcagctgt aatcttaatt
     1381 tcactgccgg agattgcatc cggcagtgtt atcccgccac aatgacctga tgatgtcatc
     1441 atacgtaagg tcactatgaa acagagccat ttttttgccc atctctcccg cctgaaactc
     1501 attaaccgct ggccgctaat gcgcaacgtg cggacggaaa atgtgtccga acacagtttg
     1561 caggtagcga tggtcgccca tgcgctggct gctatcaaaa atcggaaatt tggcggtaat
     1621 gtcaacgccg aacgtatcgc tttactggcg atgtaccacg atgcctcaga agtgcttacc
     1681 ggcgatctcc ctaccccggt gaaatacttc aattcgcaaa tcgctcagga gtacaaggcg
     1741 attgaaaaaa tcgctcagca aaaactggtc gatatggttc cggaagagct gcgggatatc
     1801 tttgcgccgt taattgacga gcatgcatat agcgatgaag agaaatctct ggtgaaacag
     1861 gcagatgcac tgtgtgcata tctgaaatgt ctggaagaac tcgcggcggg aaataatgaa
     1921 ttcttgctgg caaaaacgcg actggaagcg acgcttgaag cgcgccgcag ccaggagatg
     1981 gactacttca tggaagtctt tgttcccagc ttccatcttt cgctcgatga gattagccag
     2041 gattcaccgc tgtaagcagc cggagtctgc gtcgcatcag gcaataagcg ccggatgcga
     2101 catcaggctc ttgtcaaaac ggaaacagca tcgggatcat caccacacaa accgccatca
     2161 cgataatggt gaacggtacc cccaacttca caaagtcact gaagctgtaa tttcccgggc
     2221 ctaaaaccag tgtgttaaca ggtgaagaaa ccggtgtcat aaaggcggcg gatgctgcca
     2281 tcgccacgac catcgcaaat ggataaggcg acacacccat cgttttggca gcagccagcg
     2341 caatcggagc catcaacacc gccgttgcgg tattagagat aaatagccca ataaccgccg
     2401 acaagacaaa caaacaaccc agcatcatat gtggcccgta accgccgcca atgtccatca
     2461 gccctttcac cgccagcgcg acacctcccg ttttctgtaa tgccacagca aacggcatca
     2521 tcccaacgat caaaataatg ctcggccagt gtatggattt ataggcgctt tcagcatcga
     2581 tacagcggaa tttccccatc agcaggcagg cgatgatagc ggcaacagga ttaggaattt
     2641 catcggtcag cattaacgcc accattagca ccagacagaa aatcgcatgg ggtgcctggc
     2701 tgtgcgcggg cgatgcttca ctcacctctt ccggtaagtt cagcgctacg aaatcgcggc
     2761 cctgtttggc cagcataccg atcagtttcc agttacccac aaccaggatg atatcgccca
     2821 gcagcagagg ctcatccgcc agcgaacctt ccatcgcctc gccattgcgc tttagcccca
     2881 ccacattcag tccgtagcgg gtacgaaaac caatttcgcg caccgattta ccaataagtt
     2941 cagactcagg aattaatgaa atctctgcca tgcccacatc aagggcctgg tcagaaaaat
     3001 actcgccgcg cagtaccatc ggctccagca attgctcact acaaaattgc cggagatcga
     3061 catcagccgc agacatatca ataagcaaaa cgtcacgcgc gcgaaattca gaaaccccat
     3121 taacgttcac gataacgcga cgaaaacgcc gccagcgttc aacaccgatg acgttagcgc
     3181 cataacgctc acgtaatttg agatcgtcca gccgttgacc aatcattggc gaaccggggc
     3241 gaatagccag acgacgcgca cgcccggtca gtcgatattc acggataaga tcgcgaaagg
     3301 ttcgacgcgt ccagccttcg cgctgcgggg tctgggtatc ccctttcagc atgaaacgca
     3361 tcactaacat atacaaaata cccagcacca gcacgaccag gccaataggt gttacgctaa
     3421 agaaactgaa gccgtgatag ccttcacgca gcaattcact gttgactacc aggttcggcg
     3481 gcgtcgccac cagcgtcatc atgccgctaa tcagcccggc aaaactcaac ggcatcatca
     3541 ggcgcgacgg cgaagtttgc atacgcatgg caacgcttaa caccacggga ataaagatag
     3601 cgacaacgcc ggttgaactc ataaacgcgc caagccccgc gacggtcagc atcaacaaaa
     3661 ccaacatttt gatttcactg ttgcccgcca ctttgaccag ccatgtcccc attacagtgg
     3721 caacaccggt acggaccaaa ccatcgccaa taataaacaa ggcggcaatc aggacaacgt
     3781 taggatcgga aaagccggaa aatacttcgg ggaccgtcag cgttccgctt aatgcaaacg
     3841 cgacaataac aaacaaagcg accgcatcca tacgcactct gcccgtcgca aacaagacga
     3901 tggcaacggc cagtaatgaa agaacccaaa tcaattcacc gttcacaact tatccttgtt
     3961 aattgagggg gatgacttga ttctgccata aaaaagcccc gacgagacgg ggctaaatca
     4021 tgatcaggtg tttcactgaa taataacatc gccatttggc tgtttggtca cagtaatttg
     4081 ctccagactg tggaggacca aatcgacctc attcaggcgc ggggtatctg ccggagcgtt
     4141 aaccgcaatg acatggcaac ccgccgccag gccagaaagc acgccagcgg gagcatcttc
     4201 caccactaca cactcctgcg gcgcaagccc cagcagctgc gcgcctagca gatacgcatc
     4261 aggttctggt tttccgcgct tcactcgctc agcggtcaca aacacctctg gcgcgggaag
     4321 cccagctatt ttatggcgcg ctcgcgctac cggcatggag ccagaagtca caatggccca
     4381 cggaatacct gctttattca aatgattgag taaggcgatt gcccccggaa gtgcggtaat
     4441 accctcggtt tccgtggcct cgatttgctc cagacgcgta aactcggcgg caatatcagc
     4501 ctcggatttg cccgccataa aatggcgcaa agaggtgatc gcctgtttac cgtgaataaa
     4561 agccagcacc tcttccggcg ctaacccatg acgtctggcc cagttgctcc acgcccgttc
     4621 taccgcaggc agggaatcca ccagcgttcc atcaagatca aacagaaaac ctttgcaccg
     4681 cacgcgggcc tcctcaggcg ttgataattt gattaatttc gttggcgctc aaatggtact
     4741 gacgcgggca ggcatgccac acattaagca tgcgctgata tttttcccac attggcgtct
     4801 gggcgttaaa accgtgagtt ccggcatcaa aatgggtata gcgtccttcc acattaacca
     4861 taaagcggac ataaccgagg taacgtgctt cagtggcggc gtcaaagccg aggaaggtga
     4921 cacgacgttc atcgatggat tgctggtcct gcaaattaga ccaggaaaca tgcaacgcat
     4981 gatacatctc cataatgtcg atgatagtgc ggcaggtttc ttctttcagc tcgccaaact
     5041 cgcgatccag ttcacgcatc tgtaatccat aaccacgctc aatgattgtt tgcaggcgac
     5101 ggtaacgttc agcatttgcc ggatcgagca tagtcatcat cttgtactgg ttagacaaaa
     5161 taagacgttg cgcgttggtc atttccattg ttgactcctg tatcactcta ctacggtgaa
     5221 aaaaaaagaa ggctgagtat gccttctttt atatgcgtaa tcaggggtca attacaaatc
     5281 atcaaggaaa gttttatcca gttgtttgaa ggcgcgctta agcgtgtcag ctaatgcctg
     5341 gtaatcaggc ttgccttcaa cgggtgccaa cacctgtcca gactcctgca atttaccgcg
     5401 aacttcataa aaccagttaa ggattgcagg gggtaatggc gttacagaac gtttgcccag
     5461 ccaccacaat ccctgcatgg gtaaacttaa ggcgaacagc gcagtggcaa ctgccggccc
     5521 aagctgaccg cccagggcaa tctgccagca gagagtaaat acggcgatcg gcggcataaa
     5581 acggatcgca taacgcgtca tcttgataac gcgattttcg acaaagaccg gggcaaggcg
     5641 tttttccagc ggccacgtct ttgagtaatg ctgtccccgg cgaaacaagc taaaaaaatt
     5701 aacagaacga ttatccggcg ttgacatgct tcacctcaac ttcacatata aagattcaaa
     5761 aatttgtgca aattcacaac tcagcgggac aacgttcaaa acattttgtc ttccataccc
     5821 actatcaggt atcctttagc agcctgaagg cctaagtagt acatattcat tgagtcgtca
     5881 aattcatata cattatgcca ttggctgaaa attacgcaaa atggcataga ctcaagatat
     5941 ttcttccatc atgcaaaaaa aatttgcagt gcatgatgtt aatcataaat gtcggtgtca
     6001 tcatgcgcta cgctctatgg ctccctgacg tttttttagc cacgtatcaa ttataggtac
     6061 ttccatgtcg agtaagttag tactggttct gaactgcggt agttcttcac tgaaatttgc
     6121 catcatcgat gcagtaaatg gtgaagagta cctttctggt ttagccgaat gtttccacct
     6181 gcccgaagca cgtatcaaat ggaaaatgga cggcaataaa caggaagcgg ctttaggtgc
     6241 aggcgccgct cacagcgaag cgctcaactt tatcgttaat actattctgg cacaaaaacc
     6301 agaactgtct gcgcagctga ctgctatcgg tcaccgtatc gtacacggcg gcgaaaagta
     6361 taccagctcc gtagtgatcg atgagtctgt tattcagggt atcaaagatg cagcttcttt
     6421 tgcaccgctg cacaacccgg ctcacctgat cggtatcgaa gaagctctga aatctttccc
     6481 tcagctgaaa gacaaaaacg ttgctgtatt tgacaccgcg ttccaccaga ctatgccgga
     6541 agagtcttac ctctacgccc tgccgtacaa cctgtacaaa gagcacggca tccgtcgtta
     6601 cggcgcgcac ggcaccagcc acttctatgt aacccaggaa gcggcaaaaa tgctgaacaa
     6661 accggtagaa gaactgaaca tcatcacctg ccacctgggc aacggtggtt ccgtttctgc
     6721 tatccgcaac ggtaaatgcg ttgacacctc tatgggcctg accccgctgg aaggtctggt
     6781 catgggtacc cgttctggtg atatcgatcc ggcgatcatc ttccacctgc acgacaccct
     6841 gggcatgagc gttgacgcaa tcaacaaact gctgaccaaa gagtctggcc tgctgggtct
     6901 gaccgaagtg accagcgact gccgctatgt tgaagacaac tacgcgacga aagaagacgc
     6961 gaagcgcgca atggacgttt actgccaccg cctggcgaaa tacatcggtg cctacactgc
     7021 gctgatggat ggtcgtctgg acgctgttgt attcaccggt ggtatcggtg aaaatgccgc
     7081 gatggttcgt gaactgtctc tgggcaaact gggcgtgctg ggctttgaag ttgatcatga
     7141 acgcaacctg gctgcacgtt tcggcaaatc tggtttcatc aacaaagaag gtacccgtcc
     7201 tgcggtggtt atcccaacca acgaagaact ggttatcgcg caagacgcga gccgcctgac
     7261 tgcctgattt cacaccgcca gctcagctgg cggtgctgtt ttgtaacccg ccaaatcggc
     7321 ggtaacgaaa gaggataaac cgtgtcccgt attattatgc tgatccctac cggaaccagc
     7381 gtcggtctga ccagcgtcag ccttggcgtg atccgtgcaa tggaacgcaa aggcgttcgt
     7441 ctgagcgttt tcaaacctat cgctcagccg cgtaccggtg gcgatgcgcc cgatcagact
     7501 acgactatcg tacgtgcgaa ctcttccact acgacggccg ctgaaccgct gaaaatgagc
     7561 tacgttgaag gtctgctttc cagcaatcag aaagatgtgc tgatggaaga gatcgtcgca
     7621 aactaccacg ctaacaccaa agacgctgaa gtcgttctgg ttgaaggtct ggtcccgaca
     7681 cgtaagcacc agtttgccca gtctctgaac tacgaaatcg ctaaaacgct gaatgcggaa
     7741 atcgtcttcg ttatgtctca gggcactgac accccggaac agctgaaaga gcgtatcgaa
     7801 ctgacccgca acagcttcgg cggtgccaaa aacaccaaca tcaccggcgt tatcgttaac
     7861 aaactgaatg caccggttga tgaacagggc cgtactcgcc cggatctgtc cgagattttc
     7921 gacgactctt ccaaagctaa agtaaacaat gttgatccgg cgaagctgca agaatccagc
     7981 ccgctgccgg ttctcggcgc agtgccgtgg agctttgacc tgatcgcgac tcgtgcgatc
     8041 gatatggctc gccacctgaa tgcgaccatc atcaacgaag gcgacatcaa tactcgccgc
     8101 gttaaatccg tcactttctg cgcacgcagc attccgcaca tgctggagca cttccgtgcc
     8161 ggttctctgc tggtgacttc cgcagaccgt cctgacgtgc tggttgccgc gtgcctggct
     8221 gccatgaacg gcgtagaaat cggtgccctg ctgctgactg gcggctacga aatggacgcg
     8281 cgcatttcta aactgtgcga acgtgctttc gctaccggcc tgccggtatt tatggtgaac
     8341 accaacacct ggcagacttc tctgagcctg cagagcttca acctggaagt tccggttgac
     8401 gatcacgagc gtatcgagaa agttcaggaa tacgttgcta actacatcaa cgctgactgg
     8461 atcgattctc tgacggccac ttctgagcgc agccgtcgtc tgtctccgcc agcgttccgt
     8521 tatcagctga ctgaacttgc gcgcaaagcg ggcaaacgta tcgttctgcc ggaaggtgac
     8581 gaaccgcgta ccgttaaggc agccgctatc tgtgctgaac gtggtatcgc aacttgcgta
     8641 ctgctgggta atccggcaga gatcaaccgt gttgcagcgt ctcagggtgt agaactgggt
     8701 gcaggcattg aaatcgttga tccagaagtg gttcgcgaaa gctatgttgg tcgtctggtc
     8761 gagctgcgta agaacaaagg catgaccgaa accgttgccc gcgaacagct ggaagataac
     8821 gtggttctcg gtacgctgat gctggaacag gatgaagttg atggtctggt ttccggtgct
     8881 gttcacacta ccgcaaacac catccgtccg ccgctgcagc tgatcaaaac tgcaccgggc
     8941 agctccctgg tatcttccgt gttcttcatg ctgctgccgg aacaggttta cgtttacggt
     9001 gactgtgcga tcaacccgga tccgaccgca gaacagctgg cagaaatcgc gattcagtcc
     9061 gcagattccg ctgcggcctt cggtatcgaa ccgcgcgttg ctatgctctc ctactccacc
     9121 gggacttctg gtgctggtag cgacgtagaa aaagttcgcg aagcaactcg tctggcgcag
     9181 gaaaaacgtc ctgacctgat gatcgacggt ccgctgcagt acgacgctgc ggtaatggct
     9241 gacgttgcga aatccaaagc accgaactct ccggttgcag gtcgcgctac cgtgttcatc
     9301 ttcccggatc tgaacaccgg taacaccacc tacaaagcgg tacagcgttc tgctgacctg
     9361 atctctatcg gaccgatgct gcagggtatg cgcaagccgg ttaacgacct gtcccgtggc
     9421 gcactggttg acgatatcgt ctacaccatc gcgctgactg cgattcagtc tgcacagcag
     9481 cagtaatctc gtcatcatcc gcagctttac gctgcggata tctgaaccgg aaatagtcac
     9541 tatttccggt tttttattct cttaatttgc attaatcctt tctgattatc ttgcttaact
     9601 gcgctgcatc aatgaattgc gccatctcaa tttgcatact taccactttg ttttgtgcaa
     9661 gggaatattt gcgctatgtc cgcaatcact gaatctaaac caacaagaag atgggcaatg
     9721 cccgatacgt tggtgattat cttttttgtt gccattttaa ccagccttgc cacctgggta
     9781 gttccggtgg gcatgtttga cagtcaggaa gtgcagtatc aggttgatgg tcaaacaaaa
     9841 acacgcaaag tcgtagatcc acactcattt cgcattctga ctaacgaagc aggcgaacct
     9901 gagtatcacc gcgtacagct gttcacgacg ggcgatgaac gcccgggcct gatgaacttc
     9961 ccgtttgaag ggttaacctc aggatcgaaa tacgggacag ccgttggcat catcatgttt
    10021 atgctggtga ttggcggcgc gtttggcatt gtgatgcgta caggaaccat tgataacggt
    10081 atcctggcgc ttattcgcca tacccgcgga aatgaaattc tctttattcc tgcgctcttt
    10141 attctgtttt cacttggcgg cgcggtattt ggtatgggag aagaggccgt cgcctttgcc
    10201 attatcatcg caccgctaat ggtccggctg ggctatgaca gtattaccac cgtcctggtg
    10261 acctatatcg ccacgcaaat cggttttgcc agttcgtgga tgaacccgtt ttgtgtggtt
    10321 gttgctcagg ggattgccgg cgttccggtg ctttctggct ccgggttgcg catcgtggtg
    10381 tgggttatcg ccactctgat tggcctgatc tttaccatgg tgtacgcctc acgagtgaaa
    10441 aagaatcctc ttctgtcacg cgtgcatgag tccgaccgct tctttcgtga aaagcaggcg
    10501 gatgttgaac aacgtccgtt tacctttggt gactggctgg tattgattgt cctgaccgcc
    10561 gtaatggtct gggtgatttg gggcgtgatc gttaatgcct ggtttattcc agaaattgcc
    10621 agccagttct tcaccatggg tctggtgatt ggcatcatcg gcgttgtttt ccgccttaac
    10681 ggcatgacgg ttaataccat ggcttcatcc tttaccgaag gggcgcgaat gatgatcgcc
    10741 cctgccctgc tggtgggttt cgccaaaggg attttgctgc tggtcggtaa tggtgaagcg
    10801 ggtgatgcca gcgtgttaaa taccatcctc aacagcattg ccaatgccat tagcggtctg
    10861 gataacgcgg tcgcggcctg gtttatgttg ctcttccagg cggtatttaa tttcttcgtg
    10921 acatctggtt ctggtcaggc ggcgttaacc atgccgttac tggcaccgct tggcgatctg
    10981 gtcggtgtaa accgtcaggt taccgttctg gcgttccagt ttggtgatgg cttcagccac
    11041 atcatttacc cgacctcagc ttcgttaatg gcaacgctcg gtgtttgccg ggtggacttc
    11101 cgtaactggc tgaaggtggg cgcgacactg cttggactgc tgtttattat gtccagcgtc
    11161 gtggtgatcg gcgctcagtt gatgggctac cactaaaaat gttaagagcc gcaattgcgg
    11221 ctcttttttc attctgccgt ttcagtctct acggcttcat ttttggcatt gcgcttcatc
    11281 cacaacgcca gcgctttcag cgagtctgga gtgaactcat cgcagcgtgc ggtgatttct
    11341 tccggcgtca gccagcaaac ttcgctgact tcatcttcct gtagggcgaa gggaccgtga
    11401 gagacgcagc tgaacaacgc gccccagaca cggcaatttt tatcttcgaa atagaactgc
    11461 ccgtgctcgg caaaggggac accggcaatg cccaactctt cttccgcttc gcgacgcgcg
    11521 gattccagca gttgctcatc ggcctggact acaccgcctg cggtcgcatc taacatgccg
    11581 ggtaaaaagt cttttgtctc ggtacgacgc tggaccagaa ttttgcccat gccatcatgc
    11641 acgacgatgt aagttgcacg atgacgcaga cactgtgccc gcatttgttc ccggctggct
    11701 tgtgcaatga cttcgttctc ttcattgaca atatccaccc attcagtact tgccaaacga
    11761 cgctgttcca ttatcaggaa accttctttt tctggcgctc ttacggcgca ttttgagttg
    11821 tgggtaaatt acggattaat cgcgacctgc gcaatgatac tttgatcatt gagtgcgatt
    11881 acactaagta cgtcattatc cagcatgcca taactcgccg gattaccgcc tttaggaata
    11941 ctcaccgagc cggggttgaa gtggaaaatc tccccccgct gttctgccac tggcagatgg
    12001 gtatgaccgt acaccagcac atcgttctgg tttaaagcag gtaggttttc cgggccaaac
    12061 agatgaccat gcgtcaaaaa cagacgttgt ttttccagta atacctgttg ccacggcgcg
    12121 gttatcggga aatgcaaaag catttgatcc acttcgctgt cgcaattgcc gcgcacagcg
    12181 ataaccttat gtgccacttc attaagccgt tcagcgactt tggctggcgc gtaaccctcc
    12241 ggtaaagcat tacgcgggcc gtgattcaac acgtcgccaa ggatcaccag ccactgggca
    12301 ccactttggg caaacaactc cagaacacgt tccgtcgccg gtaacgaccc atgaatgtcc
    12361 gatgcaaaca tcagtttcat cactcactcc tcgtcgaaaa aacgtaagct atgatactgg
    12421 attctgtcgt tgctatcagc cagattgctt cgccgaaaga gcaataaaac gctggacaga
    12481 cgctcgctgc cactggaatg ttgcataatc caccaggcgt tctggcacct catcgccatg
    12541 tagtaccagg cggttaatca ttagcgccag atcagtatca gcaatgcacc attcaccaaa
    12601 taaattcggc tgacccagtg ctaacaaatg ttctgccatc gcgaacagtt tctctgcact
    12661 ggcttttccc tccgccgtta atggcgcttt tttcgcaccc gcaaaaacga catctgtcga
    12721 acgttcttcg cggatgggca tcagatcgct acgcagccag gcctgaatct gtcgtgcacg
    12781 cgcacgattt tctaaatcaa gcggataaat gcgttcccag gtcggtggcg caaatcgatc
    12841 ttccagatac tccgcaatgg cagaagattc actcaactca aaatcatcga tttgtaataa
    12901 cggcacacgg cgtgtctgac cgtaaccttg ccacgtcggc tgcaaatgtt caccgctgtc
    12961 gaggtcgatg gtcttgatat gaaacgacag gcctttttcc tgcaacgcca cccaggcgga
    13021 taacacataa ggggagaaaa aatgggcatc tgaccaaagc gtgatagcgg gtttactcat
    13081 tatgacctcg gcaataaagg gggctggcta tcaaactata gagccttttt gtcactgtca
    13141 cgtgacgaat actcacgcaa gtcgccgcgc tttctatact tgtggtgttc agatgattag
    13201 acaaccggag ctgcaatgat cgatctctat ttcgccccga caccaaatgg tcacaaaatt
    13261 acgctgtttc tcgaagaagc agggctggat tatcgcttga taaaggtaga cctggggaaa
    13321 ggcggtcagt ttcgcccgga atttttgcac atttcgccta acaacaaaat tccggcaatt
    13381 gttgatcatt ctccagccga tggcggcgaa ccgctaagcc tctttgagtc tggtgccatt
    13441 ttgttgtatc tggctgagaa aacaggactc tttttgagtc atgaaacgcg tgagcgcgcc
    13501 gccacattac agtggttatt ctggcaggta ggcggactgg ggccgatgct tgggcaaaat
    13561 catcatttta atcacgcagc ctcccaaacc attccttacg ctattgaacg ttatcaggtt
    13621 gaaactcagc gtctgtacca tgtactgaac aagcggctgg aaaactcgcc ctggctgggt
    13681 ggcgagaact acagcattgc ggatattgcc tgctggccgt gggttaatgc ctggactcgc
    13741 cagcgaattg acctcgcaat gtatccggca gtcaagaact ggcatgagcg gatccgttcg
    13801 cgccctgcca ctgggcaggc actgctaaaa gcacaacacg gtgatgagcg ttcggatagt
    13861 taacagaaac aggttctcgt gtattatttc atcctaagta aaacaacgga gaacctgcaa
    13921 tggcacaacc tgccgctatt attcgtataa agaaccttcg tttacgtacg tttatcggaa
    13981 ttaaggaaga agaaattaac aaccgtcagg atattgttat caatgtgact atccactacc
    14041 ccgccgataa agcgcgcacc agcgaagata tcaacgatgc gctgaattat cgcaccgtga
    14101 cgaaaaacat tattcagcat gtagagaata accgtttctc tttgctggaa aaattaactc
    14161 aggatgtgct cgatatcgca cgtgaacatc actgggtgac gtatgctgaa gtggagatcg
    14221 ataaactgca cgcgctgcgc tacgccgatt cggtatccat gaccttaagc tggcagcgtt
    14281 aaccgccata tcgggaggct gcatgaagat agtgatcacc ggagggacag gattaattgg
    14341 tcgccatttg attccacgtt tgctggagct gggccatcaa atcacggtaa tgacgcgtaa
    14401 cccgcagaaa gccagttctg ttctcggccc tcgggtggca ctatggcaag ggcttgccga
    14461 tcaaagcaac ctcaacggcg ttgatgcggt aatcaacctg gccggagaac cgattgctga
    14521 taaacgctgg actcacgagc aaaaagagcg tctctgccaa agccgctgga atatcacgca
    14581 aaaactggtc gatttgatta atgccagcga cacgccgccg tcggtactca tttccggctc
    14641 ggcaacgggc tattatggcg acttaggtga agtggtggtt accgaagagg aaccgccgca
    14701 taacgaattt actcataaac tctgcgccca ctgggaagaa attgcctgcc gggcgcaaag
    14761 tgacaaaacg cgagtgtgcc tgctgcgtac cggcgtagtg ctggcaccgg atggcggtat
    14821 tctcggtaaa atgctgccgc cgtttcgtct tggcctgggc gggccgattg gttccggtcg
    14881 gcagtatctg gcctggattc atattgatga tatggtcaac ggcattctct ggctgctgga
    14941 taacgagctg cgcgggccat ttaatatggt ttcgccctac ccggtacgca atgaacaatt
    15001 tgcccatgcg ctcggtcatg ctctgcatcg cccggctatt ttgcgcgtcc ccgcaaccgc
    15061 cattcggctg ttaatgggcg aatcttcagt actggtatta ggtggacaac gcgcgctgcc
    15121 taaacggctg gaagaagcgg gttttgcgtt tcgttggtac gatttagaag aggcgctggc
    15181 ggatgtcgtt cgctgatgtg gtttactgca aacatccgcc agttaactcc cggtgttaca
    15241 gggttagtgg ctttgcgcga taagatcgtc tggtgaaagt cgggtcacca tcataactaa
    15301 ctctcggtct aaacctctct ccagcatctc ctgagcaata cgcagggctt cttcgcgttt
    15361 gccaagaatt aatccatcat tccgcccttc ttcacgtaat ctgtcagcaa tagtcatcag
    15421 tttctccttt tcttgtggtg cgcgttccgc tatctcacca ataaatgcac ggaaacgctg
    15481 ggcatccccc gtttgtaata cgtaattaaa cagggctttt agctgtctgt cattagtgtt
    15541 ccctgtaact agcagcgaaa caatttggtc gactaatccc aacagatcgc gctgacgaat
    15601 atgtttctga attaactcca acagcgccat tttgcgatgt tgcataatct cgtcatccgg
    15661 caccacggta atatccacca gcggaaaagc cgatgaataa attttgcggg ctatagcagg
    15721 ctcggcaaat tcatcaagcc agcaaagtga ataaggataa ggacttctgc aaccatgata
    15781 aaacagcatc gggatcacca atggaagctc tttatagcct gcatcaagat ggttttgcat
    15841 cgccgcaatg gaatagcgca tcatgcgaaa agccattaat tcttccggct tactttggtg
    15901 ctctatcact acataaatat aacctgctcc ctcctgcgtt ttcacagacc acaagaggtc
    15961 ggaataatat tgccgcaggt cttcatcaat aaaactgttt ggttccagtt ttagcgttgt
    16021 taaatcacac agtttgcgca gcggcgcggg aagatgaata tcaataaaat cccgcgcggt
    16081 gtctggatgg cgtaaaaaag atttaaatac cgcatcatgc ggcgtggaag ttgtcgatat
    16141 tgtcatggcg ttccgtcacc cctcaaattt aagatgacgc gacaataacc acgattaata
    16201 cccaatgcag caggcagttt ttatctttgg acagcgaatt cagaactatt tttagcggac
    16261 tgcaactttt acatttcagc ggaagctgac tcgcaaattc cccttgaaaa tactccggca
    16321 gcgttagcca ccggaggggg aattatttca gcgatccctt aaggaactgt tgcagacgag
    16381 ggctttgcgg gttaccaaat aactgttctg gcgcgccctc ttcttctatt ttcccctgat
    16441 ggaggaaaat gacgtgagta gaaacatggc gagcaaagcc catttcgtga gtcactacca
    16501 ccatggtttt cccctcttct gccagttgct gcataatacg caacacttcg cctaccagtt
    16561 caggatcgag cgccgaggta ggttcatcaa acagtaaaac ttccggttcc atcgccagcg
    16621 cccgcgcgat agaaacacgc tgttgctgac caccggacag gtgcaccggg tatttcccct
    16681 gcgcacgttc gtctatcccg acttttgcca gatatttcac cgcccgctcg cgcgcttcct
    16741 gcttgctcag gcccaacacc tgaatcggcg cttccatgac gttttccagc accgtcatat
    16801 ggctccagag attgaagtgc tggaatacca tcgtcaggcg tgtgcgcagt aagcgcagct
    16861 gatttttatc ggcgactttg agttgaccat ctttgtcgcg caccagattg atcgtctggc
    16921 cattgaccac gatcgatcct tcgctcggtt tttcgaggaa gttaatgcag cgcagaaagg
    16981 tacttttccc cgatcccgac gatccgatga tgctaattac atctccggca ttcgcttgca
    17041 gtgaaacccc tttcagcact tcgtgttcgc cgtagcgttt atgcaaatcg ataacgttta
    17101 atttattctc ggacatcgta ttctcagtgc gttgaagaag gtttcacatg ctgcaaccag
    17161 cgtttttccg ctctgcgaaa gaggctgatc aggacataag agatgattaa atagagcacc
    17221 gcggcaatgc cgaaggcggt aaaaggttga tatgtggcgg cgttaatatc gcgggcaatt
    17281 ttcagcagat ccggcaccgt ggcagtaaat gccagcgcag tagagtgcag catcaggatc
    17341 acttcgttgc tgtacgccgg taacgcaata cgcagcgcag aaggcaaaat aatgcagcga
    17401 tacattttaa aggtcgagaa gccataggct ctggcggctt caatttcccc atgcggcact
    17461 gaacggattg ccccggcaaa aatctcggta gtgtaagcgc aggtgttaag cgtcagcgcc
    17521 agcacggtac agttcagacc actgcgaaaa aaggcgttaa ggaactcggt tcccttaaca
    17581 atctccagcg tgtacatgcc ggaatagaac accagcaact gaacatacag cggcgtaccg
    17641 cgaaaaatat aggtaaataa ccagattgga aactggatgt atttattact tgagacgcga
    17701 ccaatcgcca gaaacagcgc cagaactccg cctatcacta ccgacaaaat aagcagccac
    17761 agggtgatcg ccacgccagt aaagcgataa ccgtcggtcc acagcagtgg tttccagtat
    17821 tcatgtaaga tttcgatcac aggtcagccc tcttcacacc cacggagtag cggcgctcaa
    17881 ggaacagcag cacaccattg gaaacagtgg tgaaaaccag gtaaatcacg ccacagacga
    17941 tggcgaaata gaacggttcc caggtacttt tacctgccag ctgagtggct ttgagcacat
    18001 cttccaggcc gagtaacgaa acgagtgcgg tagatttgag gatcacctgc cagttgttgc
    18061 caatgcctgg cagggcgtaa cgcatcatcg ccggaaacat gatccgtcga aacacttgcc
    18121 cacgagtaaa accgaacgcc gtcgccgcct ctatgtgtcc tttcggcact gccataaacg
    18181 cgccacgaaa cgtttcggta aaataagcac cgtagataaa accgagagtg ataatacctg
    18241 cgaccatcgg atcaatatca atctgcccga cgcccatcgc ctccgtcacc gtgtttagcg
    18301 caatctgcaa accgtagaaa atcagcagca tcaacaccaa gtccggcacg cctcgaatca
    18361 gcgtggtgta cccttcgaaa atcagccccg aaagccgatt ttgcgagagc ttaccgccag
    18421 cgccaattaa accgataatt acagcgagca ctacagagct gatagccagc tccagcgtga
    18481 cgagcgcacc ctgtaaaata acacctgaaa acccatacaa catgctgcct gtcctgtcgt
    18541 gtgtggtgaa ttaccgcctc ttgtctccct ccggttaccc gaagggagcg gactttcttt
    18601 caccgatggc gattaaccac cataaacatc aaaatcgaag tactttttcg ctaatttctc
    18661 gtaagtaccg tcagcgcgca tttcggcaaa ggctttgttc agtgcctcgc gcagttcgtt
    18721 atcttcttta cgcaggccca tgccggtccc tacgccaaac agtttttcat ctttaacaga
    18781 cggaccaccg aatttgtaat ctttaccgac gggttgtttg aggaaacctt cgctggcagc
    18841 gacctcatcc tggaacgcgg catcaatacg tccggcagtc aggtcagaat aaatgttgtc
    18901 ctgcccctga taagaaacga tttcaatgcc ttttggtgcc caatgttcgt taccgaacgt
    18961 ctcctgggtg gtgccctgca atacgcctac ccgtttgcct ttcagcgact cgactgtcgg
    19021 ctgaatgtcg gaatttttcg ccaccaccag acgagaatcg gcagcgtaca gtttgtcggt
    19081 gaaggcaatt tcttgctgac gtttttccgt aatggaaagc gatgacatga tggcgtcaat
    19141 cttcttcgct tttaaggacg ggattaacgc atccagtggg ttttcgacaa acgtacattg
    19201 cgtattgatg cgtttgcaca actccttcgc cagatcgata tcgaagccaa ccagttcgcc
    19261 ttgtgaattt tttgattcaa atggcgcata ggtcggatcg gtaccgatgc ggatgttttg
    19321 cggaatcgca gcaaacgccg cagttgcgct ggagaaggcc agaaccagag agagcgatag
    19381 caccagtttt ttcataatgt cctcaactga cagtcttttt atagggaata tttacaggtt
    19441 tgttacactt atcgtgccat aaaattgacc atcaaggcaa aatatcccgc cccgttagac
    19501 gtattatttt tcattaaaaa tgcttaagta tgcatttcaa tgcaccacga tagtgcaaca
    19561 aacctggcgt gcaccatgat gatcattcca tcaggtacag tttccctgcg acgtatcagt
    19621 caccgtagac attaaagtcg aaatactttt tcgccatctt gtcgtaggta ccgtcctgac
    19681 gcagctcgcc aagcgcctta ttaaacgccg ccgtcagttc agcatcatct ttacgtagcc
    19741 ctacaccggt accgtcaccg aagtattttt tgtctttcac tgatgagcca gcaaaggcga
    19801 aatctttacc agcaggttgc ttgaggaatc cttcactggc agcaacttca tcttgtaacg
    19861 cagcatccag acgtcctgca gccagatcgg aataaaccaa atcctggttg gcataggcca
    19921 ccacatccac gcctttgcta cgccaggtct cgttagcgta tgcttcctgg gttgatccct
    19981 gcagcacacc cacatgttta cctttcagtg aatccagcgt tggctgaatc ggtgaacctt
    20041 tggcggcaat caaacgagaa tctgcggcgt acaacttgtc ggagaaagca atctcctgct
    20101 gacgtttatc ggtaatggag agcgacgaaa taatggcgtc gatttttttc gcttttagtg
    20161 aggggatcag cgcgtcaaag tcactggcaa cccaggtaca tttcacctgc atccgtttgc
    20221 acatctcgtt accgagatcg atatcaaagc caacaaagtc acctttggca tctttcgatg
    20281 agaacggcgc ataggtggta tcggttccga tacgtaccgt ctccggtagc gccgcatagc
    20341 tggaagccgc tgcggagaga ccgactaaca aagacagagc gagaatcgac ttcttcatac
    20401 ataaccctca agtgaaaatg gcttttttat gttgtgtatt gtgttgtgtg tttgcaggct
    20461 ctttcatgca ggtcttatgc catctttacg acaacagtaa cattcaacgt taaatatgtt
    20521 aataagacgt tgcattattg tcctagagtt gaagatagca ggtatggcgg ttgaatcgca
    20581 gcgtttcgat tcatcgaaag gaacaaaatg tcgaggattt gatcgcggtt gcaaaattgc
    20641 cctgaaacag ggcaacagcg gagttatgcg ccctgccagc gggcaaagag atcttcagga
    20701 agggttatcg caaactggtc aagaacacga ttaaccgtct gatttatcac atcatcaagg
    20761 gattgcgggc gatgataaaa cgccggaacg ggaggcataa tcaccgcacc gatttctgcc
    20821 gcctgggtca ttaaacgcag atggcctaag tgcaatggtg tttcacgcac gcagagcacc
    20881 agcgggcgac gctctttcag caccacatct gccgcacggg tcagtaagcc atcagtatag
    20941 ctatggacaa tgccggacag ggttttgatt gaacagggta aaatcaccat ccccaacgtc
    21001 tggaaagaac cggaagaaat gctggcagca atatcgcgcg catcgtgtgt cacatcagct
    21061 aacgcctgca cttcgcgcag ggaaaaatcc gtttcgaggg ataaggtctg gcgcgctgcc
    21121 tggctcatta ccagatgcgt ttcgatatct gtgacatcgc gcagaacctg taataaacgc
    21181 acgccataaa tcgcgccgct ggcaccgctg atgcctacaa tgagtcgttt cataaatagt
    21241 tgccctgtca gacttgcagg cagactttgc aggatttcga cgggagttgc aagtcagggt
    21301 gccagaccgg caccctgagc gaaggcatca tccttcgtta tgcatttcga gattttccac
    21361 ttcgttctga cgttgcacgg ctttggcgtc gtcattacgt aaagtatcga ggaaatcgag
    21421 gtagccctga tcaacgtctt tggtgacgta gacgccgttg aataccgagc attcaaactg
    21481 ctggatatcc gggttttcag cgcgaacggc ttcgatcaga tcgttcagat cctggaaaat
    21541 caacccgtca gcaccgatga tctggcgaat ttcatctact tcgcgaccgt gagcgatcag
    21601 ttccgtggcg ctcggcatat cgataccgta aacgttcggg aagcgaattt ccggtgccgc
    21661 agaagcgagg tacactttct tcgctccggc ttcgcgtgcc atctcgataa tctgctcaga
    21721 agtggtgcca cggacaatgg agtcgtcgac cagcaagacg tttttatcgc ggaactcggc
    21781 gcggttggcg ttcagtttac ggcgcacgga cttacgacgc agctgctggc ccggcatgat
    21841 gaaggtgcgg ccaacatagc ggtttttaac gaagccctgg cggtacggtt tgcccagaat
    21901 acgagcaatt tccagcgcga tatcacacga ggtttccgga atagggatca ccacgtcgat
    21961 atccagatct tcccattcgc gggcaatttt ctcgcccagt ttggtgccca tattcacgcg
    22021 cgcgctgtaa acggaaattt tgtcgatgaa cgagtccggg cgggcaaagt atacatactc
    22081 aaacaggcac ggattgctga ccggattgtc agcacattga cgggtaaaca actgcccttc
    22141 ttcagtgatg taaatcgctt cgcccggcgc gacgtcacgc aggaaatcaa agcccagcgt
    22201 atcgagcgca acgctttcgg aagcgaccat atattctgta cggttctcgt caatatcacg
    22261 ttttcccagt accaacggac gaataccgtt tggatcgcgg aaagcaacca taccgtggcc
    22321 gatgatcatc gccacacagg catacgcgcc gcggattaag cggtttgtag cagcgatggc
    22381 ggcgaaaatg ttgtcggctt ccagcggata gtggcggaag ttgtccagtt cgctggcaaa
    22441 gatattaagc agaatttccg agtcggaagt ggtgttgatg tggcggcgtt tttcttcaaa
    22501 cagtttttta cgcaactcgt gagcgttggt cagattgccg ttgtgggcaa gcgtaatgcc
    22561 atacggggag ttaacgtaaa acggctgcgc ttcagaggcg ctggagctgc cagccgtagg
    22621 gtaacgcaca tgaccaatgc ccatattgcc ctgcaaacgc tgcatatggc gagcttcaaa
    22681 tacatcgctc accagcccgt tcgctttacg caaacggaag cagttattgg catctatggt
    22741 gatgatgccg gcggcatcct gaccgcgatg ctgaagcacc gttaaggcat cataaatcga
    22801 ctggttaacc ggcataacac cggcgatacc gacaataccg cacatacgtc tttttcctcg
    22861 ttaagccaca tctcagagca cttacgctct gggcaagaaa cttgacgagc tttgcagata
    22921 atcaaaaaac catctgatga taaaactgaa ctgcgggatc agctgtgatt tgctccagtc
    22981 ttcgcttttc gacaccccgg taaaggagtc gagaaagaag agaatggcgg caacaatcaa
    23041 cacaccgcgc aacgcaccga aacagacgcc cagcacccga tcggtgcctg acaaccccgt
    23101 tttctccacc aactggccta tcacgaagtt cacgatagca ccaacgatca gggtagcgat
    23161 aaacagtacc gcgatggcaa tcccatttcg aaccagttcg tcttcaaagc ccgtaaacca
    23221 gactgacagg taagtgtagt aatgactggc aacaaagaaa gcacaacccc atgtcaccag
    23281 cgataacgct tcacgaacaa agccgcggat caggctaacc agagaggaaa aagcaatcac
    23341 cgcgattatg gcgtaatcaa tccagaccat atgtgtccca cgattttacg ccctgtcatc
    23401 ctgttcgggg cgcattctaa cagaaaaaga aaacgtttgc gtagggattt ccttcccgcg
    23461 catcaataaa aatggcgctg aaaaaacatt caacgccatc gactttttat gcctttgcgg
    23521 catcgggcaa tgcgtgtcgg atgcggcgtg aacaccttat ccgacctacg gttctacccc
    23581 tgtgtaggcc tgataagacg cgccagcgtc gcatcaggca agaccgtatt aattcggcgt
    23641 atagcccatt accacgccac ttaagccaga aagttgcttc aactcaccca gcgatccttt
    23701 cagcttatct ttcgaggcat caggcccaac cagaatacgg gtaattttac cctgcactgg
    23761 cgtggatggc gacgtataaa cccgataacc ggcaccgcgc agcttaccga caatctcatt
    23821 cactttatcg gcatttttca gcgcgcccag ttgcacaaca taggctttac ccgttggcgc
    23881 tgctttttct tccacgaccg gcttcgacgc aggtggtgct tcaaccttcg gtttaggcga
    23941 ctccaccggt ttcggcttcg gtggggcgac cggtgcaggt tccggttcaa actcggtgtt
    24001 attgactgca atagtggccg gatcgagcga cggtgctgcg gcatcacctg cccgcacttc
    24061 ttccgctgcg ccttccggcg gctgcgtcgg cagcgcctgg gtggcggctg gcatcatatc
    24121 aggctcatcg cgatcgcccg ctttcggcac cagcgggata gccgcgaact catcctgata
    24181 atgttttttc tgcccgtcca gcagccctgg aagtacaatc acccccagcg ccaccagcac
    24241 gatcgtgccc actaaccgat tctgaaactt acttgccacc gcttctcctc gcgtcaatca
    24301 cttccatgac atgtgcgacc gtgtggaacg aaccacacac cagcacggta tcttccgctt
    24361 tagcgtccgc cattgcggca tcccatgcct gcgccacgct atcaaatgat ttgccgttac
    24421 ccaaatgctc aagcagttgt tctgccgtgg caccgcgcgg cccttccagt ggcgcacaat
    24481 accagtcatc aaccacgctt ttcaaccagg ccagagttcc ggcaatatct ttatcatgta
    24541 gcataccgat aaccgccagc acgcgcccgt ttttcggtag cgctttcata cgcccggtga
    24601 gatattccgc cgcatgtgga ttatgcgcga catcaaaaat aacgcgtggc gactcgctca
    24661 caatctggaa acgtcccggc aaaattgcgc tggcaatccc gtcgcgaatg gcattttcac
    24721 tgacctccag cccgctggca cgcagtgccg ccagcgccgt tgcggcattc ggttgcggga
    24781 caagcggcaa cggcagattt tccagcgtgc cgtgagcatc gctaaacgcc cagtcatgat
    24841 cggtgacgga atagttccac tcaacgcccc gacgttgtaa cagtgcactt ttttcctgcg
    24901 ccacatcagc aatggtagaa ggcatttccg gctcaccgac aattgccggt ttttcgctgc
    24961 ggaagatgcc tgctttctcg cgaccaatgc tttcgcgatc tggccccagc cagtcggtat
    25021 gatccagcgc aatgctggtt actaccgcaa catcggcatc gacaatattg gttgcgtcca
    25081 gacgaccgcc cagccctact tccagaatca ccacgtcaag ttgtgcctgt ttgaacagcc
    25141 acaacgccga cagcgtaccg tactcgaaat aggtcaggga aatatcaccg cgtgccgatt
    25201 caatctccgc aaaagaggcg gtgtgggccg attccggcaa ttcctggccc tgcacacgta
    25261 cgcgctcggt ataacgcacc agatgcggcg aactgtagac gcccactttg taccctgccg
    25321 ccatcagaat cgactccagc gtacggcaag tggtgccttt gccattcgta cccgcaacgg
    25381 taaacacaaa tggcgcaggt ttcaggacgc caagacgcgc cgcgacctgg ctcacgcgct
    25441 caaggccgag atcgatagtt ttactgtgca ggttttccag ataagaaagc cacgaagcca
    25501 gaggcgacgc ggcttgagga gtgcgtttga taatcatggt atccgctgat tcgttacggt
    25561 gagaatagca aaagggcaga gccagtggcc ctgcccttat cagttatcag gcctcaggtt
    25621 cctgatctgg taccagtggc accactacgc cttcacgcgg cgcttcagga ttcggcgctg
    25681 gcagattcat caacttcgcc agaatgctcg ccagtttcag gcgcatttcc ggacgacgga
    25741 cgatcatgtc gatggcgccc ttctcgatca ggaattcact gcgctggaat cctggcggca
    25801 gtttttcgcg aacggtctgt tcgataacac gcggaccggc aaagccgatt aacgctttcg
    25861 gttcagcgat gttgagatcg cccagcatgg cgaaacttgc agaaacaccg cccatggtcg
    25921 ggtcggtcag cacggagatg tacggcaagc cgcgctcctg catttttgcc agcgccgcag
    25981 aggttttcgc catctgcatc agcgacatca gtgcttcctg catacgtgcg ccaccagagg
    26041 cggagaagca gatcagtggg cagttatctt ccagcgcctg ctcaacggca cgcacgaaac
    26101 gtgcacctac cacagacccc atggaaccgc ccataaaggc gaactcgaat gccgcagcga
    26161 caactggcat tccatacagg gtgcctttca tcaccaccag cgcatctttt tcgccggttt
    26221 ctttctgcgc agatgccaga cggtctttat acttcttgga gtcacgaaac ttcagcacat
    26281 ctttcggctc aagctcgcta cccagctcca caaggcttcc ttcatctaac aggctatgca
    26341 ggcgattacg cgctgtcata cgcatgtgat ggtcacactt cggacagacc tcaagattac
    26401 gttccagctc agcgcggtat aaaacctgac cgcagctatc acacttagtc cacacccctt
    26461 caggaatgct cgccttgcgg gtgggagtaa tgttgctttt aattcgttca atccagctca
    26521 ttagggacct ttctgtctga acctggttcg atgccagttt tatctttggg gacgcataat
    26581 gccatttttg cccccaacag accatgaatg ttgcacatta aaacataaca gcccgaaact
    26641 ttggataaaa aagtggtcga accgcggagt tactttttat tttgcggcgc gtgccgcagc
    26701 gcgtttgtgg cgaataaatt cgaaaacacc tggcaatatt gaaacaaaaa taatgccaac
    26761 aatcatcagt ttaaggttgc tctgaatgaa ggggagcgtt ccgaagaaat aacccgcgta
    26821 ggtaaaaatc agtacccata gcaacgcgcc gatgacgtta aaagcggcga aatgacgata
    26881 cgacatgtgg cccattcccg caacgaacgg cgcgaacgtt ctgacgatcg gcacaaaacg
    26941 ggcgagaata atcgttttgc cgccatgttt ctcataaaac tgatgagtct tatcaagata
    27001 actgcgacga aagattttcg aatccggatt actgaacagt ttgtcaccaa ataaccgtcc
    27061 aatggtgtag ttcaccgcgt cacccacaat cgcggcgatc agcatcagca ccaccatcat
    27121 atggacgttg agatcgttgg tttccagtga tgccaacgca ccggcaacaa acagcaatga
    27181 atcacccggt aaaaacggcg ttaccaccag accggtttca cagaacaaaa ttaagaacaa
    27241 aatggcataa acccagacgc catactccgc gaccagttct gccagatgca cgtcaatgtg
    27301 caggataaaa tcgatgagga aataaatcag gtccatatta gcctatgcct tgtactcgtc
    27361 atctttcagg ctgtaactac gttggctaca gcctgaaatg ttccgagcat tattcttgtt
    27421 agtccgccag aaatagcggg cccattggcg gttttggaag atcataccgg tcagggtaat
    27481 ccaccgcgac cagatacagc ccttccgctt ttgccgttgc tgccgccagc gttctgtcct
    27541 ttgccgccag caactctgct atccagctct ccggctggtt gtgggcaccg acttccatca
    27601 ggctgccgac aatattcctg accatatgat gtacaaaggc attcgctttg atatctacca
    27661 ccacataagg accgtgacgc gtgacattaa tatgcataac gttgcgccac ggggttcggg
    27721 actggcactg caccgcacgg aacgaggtga aatcattctc gcccagcaag cattgcgcag
    27781 cccgatgcat ccgttcagcg tccagcggtt cgtaaaaatg ggttacccct ttactcagta
    27841 ccgccgggcg cagccgatga ttgtagatga tgtagcgata acggcgagcc gtggcgctaa
    27901 atcgggcatg aaaatcatca ggtacagctt taacccaacg cacggcgatg tcaccaggta
    27961 aattcgcatt tacgcccaac gtccacgcag cgtctttgcg ctgtgcggtc gtttcgaaat
    28021 gcacaacctg cccggtaccg tgtacaccgg cgtcagtacg cccggcgcag aagacggtga
    28081 tgggttcgtt cgccacctgg gagagcgcct tttccagctt ctcctgcaca ctgcggactt
    28141 cattctgccg ttgccagccg taatacctac tgccgtcgta ctcaatgccc agcgcaattt
    28201 tataaactgg cggttgttgc tggtcggaca ttagtacaga tactcctgca ccagtttctc
    28261 ggcgattttt actgccatca gcgcgccgcc aaagcgaacg ttatcggcca ccgaccagaa
    28321 ctgaacttgc tccggcatac cgtagtcatt acgtacgcag ccaacagaaa gatgcggcgt
    28381 accagaagca tctcctacct gagtcgggaa ttcgttctct tcagagagca caatatcttc
    28441 gccctgagca aacgcatcac gcgcttcttc tgccgccagc ggacgcagtg cttcaaagtt
    28501 gaccatctgg gcatgaccgt agaataccgg tgcctggacg acgcttgccg aaatcatcag
    28561 cccttcgtcc tgcaggattt tgcgtacttc gtcaacgata cgacgttctt cacgcacgct
    28621 accttcgcta tccggcagta acggcagcat gttgaacgcc agctgacgcc cgaagaaatc
    28681 ttcttcgtca atcggaatgc cattgagtaa tttagcactc tgccccgcta acgcatcgac
    28741 cgcttttttg ccctgggcgg aggctgaaat caggctggta acgctgatac gtgataaacc
    28801 gccctgatca attagcggtt tcagtgccgc cagcagctgg ctggtcagac tgtccggtac
    28861 ggcgatgaca ttccggttgc ggtaatcggt cagtacaaac gggtttactt ccggcactac
    28921 cagcggtacg tcaggttcga gagcaaacaa tccgctgctg tcgatcacca gacaacctga
    28981 gttggtcgct tcttcaaccc aggcagcggt agcttctttg cctgcgacaa aaaatgccag
    29041 ctgcgcctgc gtccagtcaa attcagcggc atcctgcacg gtgattgtct taccaccaaa
    29101 gcgcagttgt tcgcctgcgc tttcgttacg tgccagtgca taaatttccc caaccgggaa
    29161 ctgacgttca gccagcgttt caagcagggc ttcgcccaca gcgccagttg cgcccaagac
    29221 ggcaatgttc cagccttcag acatggtggt ttactccaga aatagcaaag ctccctgcca
    29281 atgttacagc agagagcatg aagaagagat taacgtgccg gatgatgaac ggcgttaaaa
    29341 cccagtttac acagcaatga tgccgcactg gcgtcatcac aaattacata cagagatgac
    29401 cattcacggc gctcaagata gtttttgcgc agtttatcga actcacccgg tatcccggcg
    29461 actttacgca gtggcgcatc atcgcggcgc acatcataca ccaaatgcac cagccttttc
    29521 agcgtcggtt gatcgagcgg accatgcagc gtaatgcgac caaactctgg cgcgggcagt
    29581 aatgtatcca gcgcaacgtg ttgttcatgc ccaatgaatt tgctataggc ttcaaacact
    29641 tgcgtagtac cgcgtgcttt accttccagg gtatagcctg caatatgcgg cgtgccgata
    29701 tccacttttg tcagcaattc cacattaagt tccggctcgc cttcccagac atccagcacc
    29761 acgcttaact tctggccttc attcaggcag gtcagcagcg cggtattatc gacgactgcg
    29821 ccacggcagg cgttaatcag aatcgctccg ggcttcaggc tacggatcag tttttcatcc
    29881 gccagatgtc gcgttttgta cggaccatct ttaaagagtg gcgtatggaa agtcagaata
    29941 tccgcatgct ggactaattc atccagcgag cggaaatcgc cctcatcccc acggtcggcg
    30001 cgaggcggat cgcaaagcaa ggttgtaatc cctaacgctt ccagtcgtgc ctgtaaacgc
    30061 cgaccaacgt taccaacgcc cacgatcccc acggtacgct cgtgcagtga aaatccatcg
    30121 cgttcagcaa gcatcagcag tgaggagaaa acatattcca ctactgcaat cgcattacag
    30181 ccaggtgcag cggaaaaacc aattcccgcc tgctttaacc atgtgtcatc aacatggtct
    30241 gtccccgcgg tggcagtgcc aacaaattta atgggttttc ctgccagcaa agattcattc
    30301 actttcgtga ccgaacgcac catcagcgca tccgcgtctg ccagttgagc cacggggatg
    30361 ggacgcccgg gaaccgcggt cacctcaccc aaacggctaa ataagtcgcg ggcataaggc
    30421 atattttcat caacaaggat tttcacgttt gtgttacctg tatgagacga gagttaaccg
    30481 gacaagtgtg ccataatctc gcggccaggc atacttgcga agatttcagg tataaggata
    30541 cgtaatgata caacctattt ccggccctcc tcctgggcaa ccaccaggtc agggagataa
    30601 cctgccgtct ggcgcgggca atcagccttt atccagtcag caacgtactt cgctggaaag
    30661 cttaatgacg aaagtgacct cactgacgca acagcaaaga gcagaactgt gggcgggtat
    30721 caggcacgat attggtctgt cgggagattc accgctgctt tcgcgtcact tccctgccgc
    30781 tgagcacaat ctggcgcaac gtctgctggc cgcgcaaaaa agccattctg cccgccagct
    30841 tttagcgcaa ttaggggagt atttacgtct ggggaataat cgtcaggcgg tcacggatta
    30901 tatccgtcat aactttggtc agacgccgct gaatcagctc tcaccggagc aattaaaaac
    30961 cattctaacc ctgttgcagg aagggaagat ggttattccg caaccgcagc agcgcgaggc
    31021 gaccgaccgt ccgttattac cggcggagca caatgcgctc aaacagctgg tgaccaaact
    31081 tgcggcggca acgggagaac ccagcaaaca gatctggcaa tcgatgctgg aactttccgg
    31141 ggtgaaagat ggcgagttaa ttccagcgaa actgtttaac catctggtga cctggctgca
    31201 ggcgcgtcag acgctaagcc agcaaaatac gccgacgctt gaatcactac agatggcgct
    31261 gaaacaacct ttagatgcca gtgaactggc ggcgttatcg gcatatatcc agcaaaaata
    31321 tggcctttct gcgcaatcat cgctttcttc tgcccaggcc gaggatattc ttaatcagct
    31381 ttatcaacgg cgggttaaag ggattgatcc gcgtgatatg caaccgctgc ttaatccttt
    31441 tccaccgatg atggacacgt tgcaaaatat ggcaacgcgt cccgcgctgt ggatactgtt
    31501 agtcgcgatt atcctgatgc tggtctggct ggttcgttaa ccccgacgaa atgacagtat
    31561 cgtgacaata attcccagca ccgcagagat cgccccggca agaaataccg aagagtaacc
    31621 aaacgtggtc gccagcattc ccgcaagcgg cccggagacg ccgagggcga tatcctgaaa
    31681 cgcggcgtaa ccgcccagtg cggtgccgcg aacgtgtgac ggtacgcgtt taaccacctc
    31741 cacgcccagc gcaggaaaga taagcgaaca tccggctccg gttaacgccg cgcccgctaa
    31801 tgcgacccat gcacctgggg cttgccagag cagcaacaag cccaccgttt ctacaagcag
    31861 agagacgatc gccactttca cgccgccaaa acggtccggc atccagccaa acatgacgcg
    31921 catcacgaca aatgcgccgc caaacgcggt aagcgtaaag cccgccatcg cccatccttt
    31981 gctggcaaag tagagcgaaa cgaaagtccc gataaccgca aaaccaacgc cttgtagtgc
    32041 cagacctaac cctggtttcc agataagccc gacaacgctc cacagcgatg gacgttcccc
    32101 cgccagggcc ggtactttgc gcactgtgcc gttacaggcc cacgccagta agggtaatgc
    32161 cattgtggtg agcgccagtg cggcaaaacc gtaatggcta tgaatcaata ggccaagcgg
    32221 cgcaccaaca gcgagggcac cgtaaatcgc cattccgttc catgacatca ctttgccgga
    32281 atgttttggc ccaacgatgc ctaatcccca ggtcagagcg cccgtcagta actggctttc
    32341 accaaagcca agaatcaaac gcccgacgac caacagggca aatttgaacg gtgcggggac
    32401 gggcaaaatc gccgccagca gcaacgcgcc ccccgccaga ccacaagcta acatcccctg
    32461 aagcgccgaa cgttttgcgc catattgatc ggccagtcgc ccggcgtaac cacgcgtcag
    32521 caccgtagcc agaaactgaa tcccgacggc aatgccgacc atagtgttgc catagcccag
    32581 ttcatgatga acaaacagcg ggataaccgg caacggcaac cctacggtca tgtaggtgag
    32641 aaaaaccgca aaagcgatgc ggaagagcga aaaattggca gaagatcgtg tttcggtttg
    32701 gcttacagca gtcatgcatt actccagaat gcagcgcaag gcgaggagta tccccgtctc
    32761 atctctctgg tttcagggtt acggtgcgtt ggcaggattt aacgcgtacg tcttttctga
    32821 aggaaatcga caaagcggga agtttgcctg gaactggcgg cgattgtcaa tgatgtgaaa
    32881 aagggaacca tcgggttccc ttttgcgtta gtgccggagg ccgcattggc gcgtaacgtc
    32941 ggatgcggcg ctggcgcgtc ttatccgacc tactccgaat taatctttca gcttgcgcat
    33001 taccagcgtg gcgttagtgc cgccgaagcc gaagctgtta gacataactg tggtcagttc
    33061 gcgatcggtc gtttcggtca cgatgttcag acccgcagcc tgctcgtcca gctcttcaat
    33121 gttgatgctc ggggcgataa aaccgtgttc cagcatcagc agagagtaga tagcttcctg
    33181 tacgccagca gcgcccagtg agtgaccggt cattgctttg gttgcagaaa tcgccgggct
    33241 cttatcgccg aacacttcac ggatagctgc cagttctttc acgtcgccaa ccggagtcga
    33301 agtaccatga gagttcaggt agtcgattgg ggtatcaaca ccgtgcatcg ccatcttcat
    33361 gcaacgcact gcgccttcgc cagacggagc aaccatgtct gcgccatcag acgttgcgcc
    33421 gtagccaacg atttcagcgt agatatgagc accacgtgcc aacgcgtgtt ccagttcttc
    33481 gaccaccacc ataccgccgc cgccagcgat aacgaaaccg tcacggtgag cgtcataagt
    33541 acgggaggct ttttccgggg tgtcgttgta tttggtagac agcgcaccca tcgcgtcgaa
    33601 ttcacaagcc atttcccagc acagctcttc gccgccacca gcaaacacga tgtcctgttt
    33661 gcccagttgg atctgctcta ctgcgttacc gatacagtgt gcggaggtcg cacacgcgga
    33721 gctgatggag tagtttacac cgtgaatttt aaacggcgtg gcgagacagg cagaaacgcc
    33781 ggatgccatc gctttggtga ccacatacgg gccaaccgct ttcaggccgc gcgggccgcg
    33841 cattgcgtca gcgccgaaca cctgaaaacg cggggaaccg ccgccggaac ctgcaatcag
    33901 gccaacgcgc gggttattct ggtaagcttc cggagagagg ccagcatctg caattgcctg
    33961 ctccatagaa aggaatgcat aaatggatgc gtcgctcata aagcgcacaa ctttgcggtc
    34021 aatgaggcca gtggtatcca gttttacgtt gccccagacg tggctacgca tgccggaatc
    34081 cttcagctcc tgagagaaag tgatccctga acgtccttca cgcagagatg ccaggacttc
    34141 ctgctggtta ttaccgatgc tggaaacaat gcccaggcca gtaatcactg cacgtttcat
    34201 tcaatacctc tgtaagtcgc acatagagta agtttcgaat gcacaatagc gtacacttgt
    34261 acgccgaaca agtccgatca gccaattaat agagaaattt gcgcagcctg acacacatcg
    34321 ttaagatcgt cagactgccc gtcagacgag taacttacgt gaaacactac gccatacaac
    34381 ctgccaacct cgaatttaat gctgagggta cacctgtttc ccgagatttt gacgacgttt
    34441 acttttccaa tgacaacgga ctggaagaga cgcgttatgt ttttctcgga ggtaaccaat
    34501 tagaggcacg ctttcctgag catccacatc ctctgtttgt ggtagcagag agcggcttcg
    34561 gcaccggatt aaacttcctg acactatggc aggcatttga tcagtttcgc gaagcacatc
    34621 cgcaagcgca attacaacgc ttacatttca ttagttttga gaaatttccc ctcacccgtg
    34681 cggatttagc cttagcgcat ctacactggc cggaactggc tccgtgggca gaacaacttc
    34741 aggcgcagtg gccaatgccc ttgcccggtt gccatcgttt attgctcgat gaaggccacg
    34801 tgacactgga tttatggttt ggcgatataa acgaactgac cagccaactg gacgactcgc
    34861 tgaatcaaaa agtggacgcc tggtttctgg acggctttgc gccagcgaaa aacccggata
    34921 tgtggacgca aaatctgttt aacgccatgg cacgactggc gcgtccgggc agcacgctgg
    34981 cgacatttac gtctgccggt tttgtccgcc gcggtttgca ggacgccgga ttcacgatgc
    35041 aaaaacgtaa gggctttggg cgcaaacggg aaatgctttg tggggtgatg gaacagacat
    35101 tacccctccc ctgctccacg ccgtggttta atcgtacggg cagcagcaaa cgggaagtag
    35161 cgattatcgg cggtggtatt gccagcgcgt tgttgtcgct ggcgttatta cggcgcggct
    35221 ggcaagtaac gctttattgc gcggatgaag cccccgcgct gggtgcttcc ggcaatcgcc
    35281 agggggcgct gtatccgctc ttaagtaaac acgatgaggc gttgaaccgc tttttctcta
    35341 atgcgtttac ttttgctcgt cggctttacg atttattgcc cgtcaaattt gatcatgact
    35401 ggtgcggcgt cacgcagtta ggctgggatg agaaaagcca gcataaaatc gcacagatgc
    35461 tgtcgatgga tttacccaca gaactggctg ttgcggtcgg ggcgaatgag gttgaaggcg
    35521 ttacaggtgt agtaacaaat tgtggcggta ttacatatcc gcaaggtgga tggctgtgtc
    35581 cagcagaact gacctgtaat gtgctgaaac tggcgcaaca gcaaggttta cagattcgtt
    35641 atcaacacca gttacaggat ttatcccgta aggatgactg ttggttgctg aattttgcag
    35701 gagatcagca agcaacgcat agcgtggtgg tactggcgaa cgggcatcaa atcagccgtt
    35761 tcagccaaac atcgtctctg ccagtgtatt cggttgccgg gcaggttagc catattccga
    35821 caacgccgga actagcggag ctgaagcagg tgctgtgcta tgacggttat ctcacgccac
    35881 aaaatccggc gaatcaacat cattgtattg gtgccagtta tcatcgcggc agcgaagata
    35941 cggcgtacag tgaggacgat cagcagcaga atcgccagcg gttgattgat tgtttcccgc
    36001 aggcgcagtg ggcaaaagag gttgatgtca gtgagaaaga ggcgcgctgc ggtgtgcgtt
    36061 gtgccacccg cgatcatctg ccaatggtag gcaacgtgcc cgattatgac gtaacgctcg
    36121 tggaatatgc ttcgctggcg gagcagaaag atgaggcggt aagcgcgcca atttatgacg
    36181 atctttttat gttagctgct ttaggttctc gcggtttgtg ctctgccccg ttgtgtgccg
    36241 agattctggc ggcgcagatg agcaacgaac caattccgat ggatgccagt acgctggcgg
    36301 cgttaaaccc gaatcggtta tgggtgcgga agttgttgaa aggtaaagcg gttaaggcgg
    36361 ggtaatctgc tttggcattg tttgtcggat gcggcgtaaa cgccttatcc gacctacgcg
    36421 ttacctgtag gcctgataag acgcgataag cgtcgcatca ggcatggtgc tccagacgtc
    36481 acaacttact gctgtgaggc ctgctgaaac aggtgttccc acatatcggt aaccagcgcc
    36541 tggtcacgcg gcgacaattc acccgcgcca atggcttttt ccagactctg gctaacggtt
    36601 gtatgtaccg cctgggcgga gtggtcatca ccactttcca gttctgcgat ggctaatgtc
    36661 aggtggccac gcaaataccc actggcaaac aactcatcat cactggcatg gtcaaccata
    36721 ccgtcgatta atgccagaat gcgtgattca aactccgcga tcatcttctt tcctcattgt
    36781 aaaacgtggc gctggcttca gttgagcgct tccggccacg ggaactgttc cgccgtaagt
    36841 tccggcgtgt gataataatt ttgtagtgct ttaatgaagc gtgccggacg ttcgggaatg
    36901 ccgtttgcca gataatccat tacctgcgca tgtacccgcc gttgaaacac cacgcggtcc
    36961 ggttcgaaat ccccttccag attgtcgcag ctaacattaa acggatatcc cgccgccacg
    37021 cagaacaacc agtccagcgc ctgcggcttc acttcaacat cttcaaactg gctttgcgtc
    37081 tgggcatcgc gaccgtccgg gcaataccag tagccgaaat caacctgctc acggcgcgct
    37141 tttccggcaa tacaccagtg cgaaatctca tgaattgcgc tggcataaaa gccatgagca
    37201 aagacgattc ggttatacgg tacttccgca tcagcaggaa gatagatcgg ttcgtcgtcg
    37261 cctttaatca gacgggtatt aaattcatcg gcaaagcagc tattaaaaat ctcaatcaac
    37321 tgctcatagt ggtgtgtact gttcattagt tcatccccaa ccagtggagg atctcctgtc
    37381 cgtggctatc gtaaagtaat tttgcactca tcaccgccga gacgataacg atcatcgggc
    37441 ggatcagctt ttgtcctttg ctcaacacca gtcgtgaacc catgcgcgcg ccgaggaact
    37501 gccccaccag catcacaaag cccgtcgccc aaatcacttt gccgccgaga ataaacaaca
    37561 gcagaccgcc aatgtttgac gttgcgttaa gtaatttggc gtgagcagtg gctttggcga
    37621 ggttgaatcc gcacagcgta acgaaggcca gcgcgtaaaa cgatccggca gccggaccaa
    37681 agaatccatc gtaaaaaccg acgcagcccc ccgccaccag cgcaaatggc agtccataca
    37741 tccggcgctg gcgatcttct tcacccagct ttggcatcag caagaaatag aggccgatac
    37801 aaatcaccag aatgggcaaa atctgccgta agacatcagc ctgaacatac tgcaccagta
    37861 atgcgccgct cattgagccg acaaaggtca tggcgatatt gagtttctga tcgcttaaac
    37921 taaccacttt gcggcgaata aagtagatag tggcggaaat agagccgccg caggcttgca
    37981 gtttattggt tgccagcgcg ttagccggag acatccccgc cgccatcaat gccggaatgg
    38041 tgagtaaccc accgccaccg gcaatcgagt cgataaatcc cgccagcatg gcgacaaaaa
    38101 agagaactcc cagcagcagc ggggaaacca taaacaggct attaaacgtt tccattagat
    38161 cacgtgctca tccagtagcg cctggcagga aggcggcaac ggaggcggtg tcttcttctc
    38221 aggctttgtt gttcccggtt ttggtggttc aaaccagctt tgcagttctg ccccgcaacc
    38281 atcgcccggt ggcggtaaag gttgatcttc acactccagg ctatcggcag gacaacgtag
    38341 tcgtacatgc atatgggcgc gatgctggaa ccagggccgc actttgcgta accagtcgcg
    38401 atcggtgccc gcatcaaggc aaagttgttg tttaatcgcc ggattaacaa aaatgcgcgt
    38461 gacgtctttg tcctgcgcgg cgagtttaat caagctgaaa atttccggct tccacagcgt
    38521 cgggacaacg tgtttaccgt cgcgggaaac taaatctaac gcttgcgggc gcaagagctg
    38581 cgcggaggtc cagcgtgttt ttggcagttg caggaagata tcgacatcca gtccagtctg
    38641 gtggctggcg tgaccgccgt tgaaacgacc accagcaggc atccccatat cgccaatcag
    38701 caccgtaccc atacccagat tgctcacctg gcgactcaga cgctggataa acatcaccag
    38761 atccgggtgt ccgaaatagc gacgctgatc ggtacgcatg acctgataat gttcggactg
    38821 tatcggcagc gtgtcagcac cgacgataca gccatttgaa aaactgccta tcgattgtgc
    38881 actacccggc acaggttggg ttattttttg ccacggcgtc gctgccaggc tggcgctact
    38941 ggcaagcagg gccagcagca caatcgcagt tttgttcatt tttaccagcg tggaatatca
    39001 gtcttcacat cagcattttg cgcccgttgc cgtaacaggt gatccattaa aacgatcgcc
    39061 agcatcgctt cagcgatcgg caccgcgcgg atcccaacac acggatcgtg acggcctttg
    39121 gtgatcatct caacttcttc gccaaagcgg ttaatcgtgc gccccggcac agtaatgctt
    39181 gaggtcggtt tcagcgccat atgggcaatg atttgctgcc cgctgctgat accaccaaga
    39241 atgccgcccg catggttgct ctggaaaccg tctttggtga tttcgtcacg gttctggctg
    39301 ccacgcagcg ctaccacgtc aaaaccatca ccaatttcca cacctttcac cgcgttaatg
    39361 ctcattagcg catgggcgat gtcggcatcc aggcggtcaa aaaccggctc gccaagtccg
    39421 gcgggtacgc cactggcaac gacggtgact ttcgcgccga tggagtcacc ctcttttttc
    39481 agcgcgcgca tcagttcatc taacgcgtcg attttgtccg ggtccgggca gaaaaacgga
    39541 ttttgctcga cctgcgacca gtctttgatt tccagcggaa tatcacccat ctgggtcagg
    39601 cagccgcgaa tctcaatgcc aaatttctcg gcgagatatt ttttggcaat cgcccctgct
    39661 gcaacgcgca tagcggtttc gcgggcggaa gaacgtccac cgccgcgata atcgcgcagg
    39721 ccgtattttt gttcataggt gtaatcggca tgacctggac ggaaaacgtc cttaatcgca
    39781 ctataatcct gagaacgctg gtcggtattt tcgatcaaca atccgatgct ggtgccagta
    39841 gtaacacctt caaaaacacc ggagagaatt ttgacctgat ccggctcgcg gcgctgggtg
    39901 gtatagcgcg atgtcccagg tcgacgacga tcaaggtcat gttgcaagtc cgcttccgtc
    39961 agcggaatgc ctggcggaac accatcgacg atgcagccga gcgccagccc gtgcgattcg
    40021 ccgaaggtgg ttacgcgaaa gagttgtcca attgtgtttc cagccatcac ggctccgtta
    40081 ttgttgtgtt tgcgtgttta cttaatcttt ataaatcgcg aaatgttctc gtgcggcaat
    40141 aagctgctct ttagtgagca taaacacacc atcgccgccg ttatcaaact ccagccaggt
    40201 gaacggaaca tccggatatt gttccataag atgtaccatg ctgttgccca cttcacaaat
    40261 cagcacgcca tcatcagcga ggtaatctgc cgcgttacca agaatgcgac gcgtcagttt
    40321 caggccgtca gtgccagatg ccagaccgag ttccggctcg tggcggtatt cgtttggcag
    40381 gtcggacata tcttccgcat cgacatacgg cgggttggtg acaatcaggt cgtactgcac
    40441 tttcggcaaa tcacggaaca agtcggaacg aatcggaatg acgttgtgga tcaaaccgtg
    40501 ttcttcgatg ttctgttcag caaccgccag cgcgtcggga gagatgtcta ccgcgtcgac
    40561 ttccgcttcc gggaaggcat aagcacaggc aatggcgatg cagccgctac cggtacacat
    40621 atctaaaatg tgctgcggtt gcttgctgat gagtccggca aatttattgt tgatcagttc
    40681 accaatcggc gagcgcggca ccagcacgcg ttcatcgacg taaaattcat ggccacagaa
    40741 ccaggcttta ttggtcaggt aagccaccgg aatgcgttcg ttgacgcggc ggatcacgcg
    40801 ctcaacaata cggtgttttt cgctggaagt cagacgcgcg gtgcgcatat cttccggaat
    40861 atccagcggc aggtagagtg aaggcaacac cagttgcacg gcttcatccc acgggttatc
    40921 ggtgccgtgg ccgtaccaga tattcgccgc gctgaagcgg ctcaccgacc agcgcaacat
    40981 gtcctgaatg gtttgcagct catttactgc ttcatcaacg aaaattttat ccacgtattc
    41041 ctccagggca tgatcgcaat aatttcggcg gctagtttgc catgaagatg acgataaatc
    41101 agcattcacg cgcggtgagt gaggaaaaat acgtttaaaa cgatcgattg tgctacgagt
    41161 caggtaaact gtaggaaaat tagaaataga gacagttaaa tgaaaaagaa aacaacactt
    41221 agcgaggagg accaggcgct gtttcgccag ttgatggcgg ggactcgcaa gattaagcag
    41281 gacacgattg tccaccgacc gcagcgtaaa aaaatcagcg aagtaccggt caagcggttg
    41341 attcaggagc aggccgatgc cagccattat ttttccgatg agtttcagcc attattaaat
    41401 accgaaggtc cggtgaaata tgttcgcccg gatgtcagcc attttgaagc gaagaaactg
    41461 cggcgcggtg attattcacc ggagttgttt cttgatttgc acggtctgac gcagttgcaa
    41521 gccaaacagg aactgggggc gttgattgcc gcctgccgcc gtgaacatgt gttttgtgcc
    41581 tgcgtgatgc atggtcacgg gaagcatatt ttgaagcagc aaacgccgct gtggctggcg
    41641 cagcatccac atgtaatggc atttcaccag gcaccaaaag agtatggtgg cgatgcggct
    41701 ttgttggtgt tgattgaagt ggaagagtgg ctgccgcctg aattgccctg aatgctggca
    41761 tcgaatgctg tagagggaaa gttcgcctta gccatcatat cataaaacag acgccggaaa
    41821 tgacgacagg gccagatggc cccgtcgact tgtgaaaata tcgtcactac aattcattca
    41881 gtacatactg gaatcggcac gtagaatcaa tcggttgtcg gctactggtg ttcacattta
    41941 ccacgatatc atgtagctgt ttatcggtta ccggatgccg tgtgggagaa accgttaccg
    42001 ttgatttatc gcaaccgtag gaggtaaagg ttaacagttc cgagaccacg cggcagtatg
    42061 cacctctgct atcaaaaacg ctgccatcac agagtttgcc atcttccatc aggtaggtcg
    42121 aaagcgtggt ggtgattgtc gagccattgg attgcaactg taacgcctta ttgtggctga
    42181 cgtaattctc actccacgag atgtcatacg acgcgacagg gatcgtcgga catactgtgg
    42241 catggttcgt tggcccagta tatctcccga atccgggttg atctgtgatc gtataaaaag
    42301 aagcgagcca cgggcaacca taactgtcgg actgaacggt tatcttaaac gtctcatatg
    42361 cacactgaaa tgtcgccgta tttatcccag gacaattcga gctatcagca ttaccgtagt
    42421 cagttaagat caacactggc gctggtcccg accccgtcca cgaaataacg ctggcttgct
    42481 cgttcttcct tatggcatcg ttgtacaccc cataggccgc ctcgttggga gttaacaccg
    42541 tgacatacaa acctccggtg ttatgaatag acattttccc cttcccataa taaatcttca
    42601 cactgtcgaa actcgcgctg gtgagtttat ccccggcata gctctttgga acgctcgcac
    42661 tcatcaccag caacaccagg ctcgcaaaaa tcattgtcca ttttttcatg ttaaaaatac
    42721 tcaaaggttg ccgtcgccgt agcgctgaaa tcaccggacg tcacatcccg cccgcccttc
    42781 gcctgtagcc aggcattaaa attgagactg ttattgccct ggtttagcgg gaacgtgaca
    42841 cccgttgtgc tgttgactgg caccgccgcg cctgccgccg tttcaatacc aatcccagct
    42901 ccggatgcag aagatgaggc gtcaaaggcc agaaatccgg gcagttcgct atcttccgtt
    42961 cccgtcagcg ttaccctgac gctaaacagc gtggagcttt tgcaatcctt caattgaaat
    43021 actaacggta ctcgctctga ctgcccgagg ttcattaaat cgctggcggc aatggtggga
    43081 aagtgcacct ccgccaacag gctaccgttg atcactggcg tacaggattt accgagcaaa
    43141 ttaccggtaa agtgcaggtt gtcgaccgcc ggggcagtaa cgctgtacaa cagcatcagg
    43201 ctacagataa aaaccaacgc acgctttttc ttcattgata atccacctgt aacgtggccc
    43261 aggcttcaaa gtctgcctca ggcaaatcga caccgctctt tttgaccggt actgcggtta
    43321 acaccggtaa ggccgtttca ttcaccacca gtgccgtatt gagcttaaag ctctgccctg
    43381 cctgtttaag ccgaatcgcc aggccagaga tatcggtgct aaccgcggca tcgtcaaagt
    43441 cgctctgcgt accgctccag ctcaatttca tttccattga tgggtcgcga accgccgtat
    43501 cacaggtaat ggaatacggc acggtttgct catagttact gccattaatg tcatcaacaa
    43561 aaacatcctg aaaggttatt tcaatggttt taccgttgct aatagtgcag ttaggtggct
    43621 gcactaaata gccgtgaaaa gagacatttg agagtgccat cgagctaaag ccccacagca
    43681 taagaagtga gatccgtttc attggtaatc caccacaatt gtggcactgg cgttaaattc
    43741 cgctgccgat aaggttgtac cactctgttt caccggcact gcttcgagtg cagggacgcc
    43801 cgaggaaaaa ttaaacggca accatgaacc agacgttaag tcgagtgttg agtgatcact
    43861 acttttctga acccgaatac caaaccctga aatacctgtg ctgataactg tttcaccgtt
    43921 gatcacaacc gttgttgccc gcatctgtaa acgcaggtca tccattgcca ggttgttaca
    43981 aacaagcgag taatcaatgg gttgtttata acctacccca tcgatcttat tgatgaatac
    44041 attaccaaac tcaaccgtcg caccattaac cgtgcaagga ggaggggcat caacgagcac
    44101 tcgtaaactc aaacttactg atttactccc tgcaactgca aagtcgctac caatacaaca
    44161 catcagtaac catgttcccg cccagactga acaagatctt ttcatactta cttccccgtc
    44221 attaaccttt gcggctctgc tcttcatcaa cctgacaact gttgccgcta caggtgaaaa
    44281 agagtggcat ccgtgcgcca tagtcgttga cataggtcag caccggcacg ctagccatct
    44341 tcaccttgag cggtgcggag gtttttggcg acatcactat cggcgagaaa ccgtctgccg
    44401 gattgccatt tttttgcgcg ctggcattac tgaaaatgac gtaatacgga gtagggttat
    44461 tgacggtaaa ttcctgaccg ttacgggtta aggtcacttt atgctgccat gggttttgca
    44521 tgctagcttt ttccagggct ttaggtcgcc agaaaagttt gatgcgtgtc tgcagcgcaa
    44581 tttgtaacgt attggctttg ttcgatttcg gcggaatttc acgcacattg aaataaaata
    44641 tcgattcacg atcggcaggc aatttatgga tatctggcaa tccctgaatt ttcacctgac
    44701 cattcatcat tgcatcaatg cgctgtacgg ggggcaatac tgccagcggc gaactaatct
    44761 tgacgccctt ttcatcttcg atccagcttt gtgccagata aggctgtttc gggtcgttat
    44821 tacgcagcgt gacgctgatg gatttatcgc tttcattaaa aatcaggcga gtacgatcgg
    44881 gcgttactga cgccagagca gaaaaactta ccgttgcagc cagtaacgac agtaccgttg
    44941 ccaatgtttt gacagagaga tgtttagtca tggatcattc cttaatcacg ttattggttc
    45001 gatgaaacag acatcggcgg ttccattggt gtcacctgtt gtgtctgctc ctgaattaca
    45061 ggttgaatat catgcgttat gacgggagca cttccccctt tttgctggca tggcaacaac
    45121 aagccattga acaggtcatt cggtaacgga tctggcaggc tgatatcgca gtgagattct
    45181 ccctcccaga aaacggtcat atgttcaccc ggcttaacgc ctgcaagata aacattacct
    45241 tcgtcatcaa ccagcccaac ttgttgttga ttatcgtttt tcacttccgc cccaaatggc
    45301 ggatggctgc catcctgcaa gcgcagcact accatcgcct tctcaccgct gatcaccgca
    45361 aatttacggt agccaatagc gccttccgtc agcgttgcct ggaccaccga ttgggtagct
    45421 tcggcgtttt ccggtagttt gttcaaatcg atataagcct ggttacggta gtaatcatta
    45481 acatctgcca ccaccgcttt cccgaacatg ttggtataaa cggcggaacc attaccttcg
    45541 accggcacat tagcaacgcc atcggcgtcg attagcagac gtgtgcctcc catattttgc
    45601 gtacgatgca acgccccgcc gtgagcagtc agtgttacac cgccctgtaa ggagaccccc
    45661 gcagatgtgt attgcccctc atggtagttt gcactcaggt caacttttgc cagcgtaccg
    45721 tcatgactgt aataaccatc aagactggtg tgattttcac tggttccggc gctcaacgta
    45781 tagtggctag cgtcatcgat gcggtgatac aaactcgcct ggctgctgtc ggcaccactg
    45841 ccataattgc cgttgtaact gatggtgctg ttgtcacccc atggcaaact caaggacaga
    45901 taaacacctt tgtcggcact tttatcatat tcgtagcgat aaccggtgag agaaacgctc
    45961 aggttgcgaa gacttcctaa attgaaataa tgagaaagca tcatgttgta gttggtctga
    46021 tcctcgcgat cccaataggt atggtgagaa taattgagat agaccgaaac gcgcgcatcc
    46081 ctaaagttct ggttgtaagt gaccgtgtac atttccttgt cgttacccgt gcgcgcacgg
    46141 tcggtgtcgt tcgcatccag atactcactc atggtcatgt agtttttttc agagaagcga
    46201 tatccggcaa acgtcacccg actgttgagt tcatcgaaat ctttggcata gctgacgcga
    46261 taagagttgc cgtccagcgt tttgttgcca tacacgctgt tatcgtcgag ttgaacacga
    46321 gagtgagtga catcaaacgc tagcgctccg aacagcgcga ggtcacgacc gaggcctaac
    46381 gctgccgact ggtagttttc atcggccaat gccccgccgt agagcgacca gccattcgca
    46441 atcccccatg acgcctcacc gcccgagaag aagctgcctg caacctgatg gtcccagttt
    46501 tgtggacgcc ccattgtcac tttgtaacgc acctgaccgg ggcgggtcag aaacggcatg
    46561 gacgccgtgc tgacgtcata ttcctgtacc tgaccgtttt gttcttcaat gcggacatgc
    46621 agcgtgccag atacggaatc gccaatatcc tgaatgcgga atggacctgc gggcacctgg
    46681 gtttcgtaaa tgacacggcc catttgggtt acggtcactt tagcggtagt gtgtgcaacg
    46741 ccggaaatat cgggtgcgta gccgcgcatg tttggcggta acatttgatc atcggtactg
    46801 atgctgccgc caatgtaact gaagccatca aaaatatcgg agttgagata atcttcaccc
    46861 agcgaaagtt tggcttttaa tgacggcaag gcgcgccagg cgtagtaacg gctccactcc
    46921 cagttttttt gcgtaccgct atcatcagtg tcgccgtcat cgttactgcg ggtatgttgg
    46981 taatcgctct gccagtcggc tcgtaagcgc cacggcccca cgttaacgcc gaccgtacca
    47041 ttaccgctga tgtcgttagt gtcatccccc ccattttcat gtcgggtttg tgcgttgata
    47101 ctgtaatcgg caataagccc aggaatcccc tcatcccaac gcgaaggtgg gtcccagtca
    47161 tcgtcagtgt attcgagata tgcctgtggc acagaaacta agagcgcaga ttgacttaaa
    47221 tcgcccttaa tttccatacc tcctatttgg tcagttttca gacattgtcc atcatgaccc
    47281 cattgcaaac tcttcgcaaa atcatctttg aagccaaact gggctatcag ttcaggtggt
    47341 aagcaggcat atgatttatt cggatcgttt tcagctacat accaatagat atcgtattca
    47401 tcaggtaatg ggtgattatt tacatgaaca cgtaaattgt atttccctgg ttcaacatac
    47461 cctttttgag aaaaacgccc cagatcaatt ttggtatttc cttttaactc cagaaatcga
    47521 gcatcaaatt gaatggtatc atctgccagt acattaaaag gactcccgga aattgccaga
    47581 gcaatccacc atgaaagccc ccgtaaacga agaaaagaat gttcaggcat ctacgtatcc
    47641 atccatgatt attataattt agttatcttt tatttataaa ccccataaca ttgcagttat
    47701 gggggaacag cataattaga gatatgtaat ctggaacgtc gttagcgttt caaacggcgc
    47761 tggggttggt gttaccgctg cagtttcacc tactaaccag gctttaaagt tcaacgtttg
    47821 tttctgtttg ccttttcctg ttgaactgtc catcgcgatg tcctgatcga tgcctgcccc
    47881 caggtcataa ccagtaccac gttggtcgcc aatcaccaga cttacatttg gaatttcagt
    47941 tccattatcc atgttagaca gggaatagtt ttttgagttg ccggagtacg gattaccagt
    48001 gaatgtcgtg gtcattgtcg tttcagtatt gaaattacaa tcctgtaaac gaatctggaa
    48061 atctttaggt gtggtagtct tgccaccggt taaagaacct gtaccaatgc tgcccatatc
    48121 cacttccagt ttatgatcgt caggaacaat cgagcacgga gagtcttcaa ttgtgccgta
    48181 aaatctcact gtaccgttgt tacctgcggc aacgactgat gctgaagtca gcgcccccat
    48241 gagcattgcc gtggcaactg ctgcttttac aaacttactc attatttgtc cttaaatcat
    48301 aaataattag aatataagcc aactgaaata ttgacgcaga gcttcttcaa tttttacagc
    48361 gacttttaac cagccaatga acagattttc agctcctgaa aaaacaggtt ccttgatata
    48421 atattctttg gcgtgccgaa catattgata ttttaatatg actgacgcaa tttagatt
//
LOCUS       DAPHCV010000022        47604 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.022, whole genome shotgun sequence.
ACCESSION   DAPHCV010000022 DAPHCV010000000
VERSION     DAPHCV010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.022"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1880)
                     /gene="espL1"
                     /locus_tag="RH033_001283"
     CDS             complement(<1..1880)
                     /gene="espL1"
                     /locus_tag="RH033_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HDV2990498.1"
                     /translation="MSQNDIIIRTHYKSPHRMHIDSDIPTPSSEPINQFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFTINFATSPIHAENILITLHYKKEII
                     SLLLETTPIKANHLRSILDYIEQEQLTAENRNHCMKLSKKIHREKTIQPTVNLNGSAF
                     FSQSPSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRSLL
                     RHPPAGSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQSAYGQ
                     LNYVVSVYDPNDTNVAVRGTHRTARGFLSLDKFISSGPDAQTWADRYVRNCAIAFLPL
                     LPEGVPGAIFAGIASRMPFAPIHPSAMLLIMATGQTQQLIALFKQLPILPEKEIIEII
                     TAQNSVGTPALFLAMMNGHTDNVKIFMQEIQSLVDNHIIHEDNLVKLLQTKSANETPG
                     LYISMLYGFDEIIDIFLNALTTPITQELLSKKMVMDILAMKTRDGEPGLYAAMENNHP
                     LCVTRFLSKVYGIAVKYNLSKINIMDLLKGATAYGTPALYIAMSKGNKDVVLSYISTL
                     GTFAKKYSFSQCQLFTLLAAKNHDNMSAVHIAIHHNHYKTVETYYAAINVISQSLSFS
                     AD"
     gene            2403..4331
                     /gene="thrS"
                     /locus_tag="RH033_001284"
     CDS             2403..4331
                     /gene="thrS"
                     /locus_tag="RH033_001284"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDV2990499.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRTKLKEYQYQEVKGPFMMDRVLWEKTGHWENYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            4335..4877
                     /gene="infC"
                     /locus_tag="RH033_001285"
     CDS             4335..4877
                     /gene="infC"
                     /locus_tag="RH033_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDV2990500.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            4975..5172
                     /gene="rpmI"
                     /locus_tag="RH033_001286"
     CDS             4975..5172
                     /gene="rpmI"
                     /locus_tag="RH033_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDV2990501.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            5226..5582
                     /gene="rplT"
                     /locus_tag="RH033_001287"
     CDS             5226..5582
                     /gene="rplT"
                     /locus_tag="RH033_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDV2990502.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    5699..5823
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            5705..5749
                     /gene="pheM"
                     /locus_tag="RH033_001288"
     CDS             5705..5749
                     /gene="pheM"
                     /locus_tag="RH033_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HDV2990503.1"
                     /translation="MNAAIFRFFFYFST"
     gene            6033..7016
                     /gene="pheS"
                     /locus_tag="RH033_001289"
     CDS             6033..7016
                     /gene="pheS"
                     /locus_tag="RH033_001289"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDV2990504.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            7031..9418
                     /gene="pheT"
                     /locus_tag="RH033_001290"
     CDS             7031..9418
                     /gene="pheT"
                     /locus_tag="RH033_001290"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDV2990505.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPIAVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAMLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            9423..9722
                     /gene="ihfA"
                     /locus_tag="RH033_001291"
     CDS             9423..9722
                     /gene="ihfA"
                     /locus_tag="RH033_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HDV2990506.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            9823..10803
                     /gene="btuC"
                     /locus_tag="RH033_001292"
     CDS             9823..10803
                     /gene="btuC"
                     /locus_tag="RH033_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HDV2990507.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADVVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            10866..11417
                     /gene="btuE"
                     /locus_tag="RH033_001293"
     CDS             10866..11417
                     /gene="btuE"
                     /locus_tag="RH033_001293"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HDV2990508.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            11417..12166
                     /gene="btuD"
                     /locus_tag="RH033_001294"
     CDS             11417..12166
                     /gene="btuD"
                     /locus_tag="RH033_001294"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HDV2990509.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            12244..12708
                     /locus_tag="RH033_001295"
     CDS             12244..12708
                     /locus_tag="RH033_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDV2990510.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            12955..13668
                     /gene="ydiV"
                     /locus_tag="RH033_001296"
     CDS             12955..13668
                     /gene="ydiV"
                     /locus_tag="RH033_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HDV2990511.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAD
                     ELLKYPFIELLINENYPHLDEGKDNRDLFSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            13731..15167
                     /locus_tag="RH033_001297"
     CDS             13731..15167
                     /locus_tag="RH033_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HDV2990512.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQRLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLADDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRVRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(15171..15362)
                     /gene="hemP"
                     /locus_tag="RH033_001298"
     CDS             complement(15171..15362)
                     /gene="hemP"
                     /locus_tag="RH033_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HDV2990513.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(15494..16540)
                     /gene="aroH"
                     /locus_tag="RH033_001299"
     CDS             complement(15494..16540)
                     /gene="aroH"
                     /locus_tag="RH033_001299"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HDV2990514.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(16697..17530)
                     /gene="ppsR"
                     /locus_tag="RH033_001300"
     CDS             complement(16697..17530)
                     /gene="ppsR"
                     /locus_tag="RH033_001300"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HDV2990515.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            17863..20241
                     /gene="ppsA"
                     /locus_tag="RH033_001301"
     CDS             17863..20241
                     /gene="ppsA"
                     /locus_tag="RH033_001301"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HDV2990516.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(20298..21944)
                     /gene="fadK"
                     /locus_tag="RH033_001302"
     CDS             complement(20298..21944)
                     /gene="fadK"
                     /locus_tag="RH033_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HDV2990517.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGATYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKTGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSALSLSQIIADNTPLTTAITVHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPAACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFIHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEDVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(22001..22294)
                     /locus_tag="RH033_001303"
     CDS             complement(22001..22294)
                     /locus_tag="RH033_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HDV2990518.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIEFRYG"
     gene            complement(22291..23580)
                     /locus_tag="RH033_001304"
     CDS             complement(22291..23580)
                     /locus_tag="RH033_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDV2990519.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLDAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVSASDPHHYAVGVKEVIGLTPEQINDRFNVTGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSVSLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(23635..24573)
                     /locus_tag="RH033_001305"
     CDS             complement(23635..24573)
                     /locus_tag="RH033_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HDV2990520.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQTV
                     MPYGPKCIYVLAQNDALQRTENYAESIAALLKDKHPSMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSN
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(24593..25357)
                     /locus_tag="RH033_001306"
     CDS             complement(24593..25357)
                     /locus_tag="RH033_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HDV2990521.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     TAVEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            25673..26584
                     /locus_tag="RH033_001307"
     CDS             25673..26584
                     /locus_tag="RH033_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDV2990522.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNIELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLAG"
     gene            complement(26627..27778)
                     /locus_tag="RH033_001308"
     CDS             complement(26627..27778)
                     /locus_tag="RH033_001308"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HDV2990523.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPEPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(27792..29387)
                     /locus_tag="RH033_001309"
     CDS             complement(27792..29387)
                     /locus_tag="RH033_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HDV2990524.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNQEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIVFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(29532..30290)
                     /gene="aroD"
                     /locus_tag="RH033_001310"
     CDS             complement(29532..30290)
                     /gene="aroD"
                     /locus_tag="RH033_001310"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDV2990525.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIASVKSEALAYREADFDI
                     LEWRVDHYADLSNVESVIAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(30321..31187)
                     /gene="ydiB"
                     /locus_tag="RH033_001311"
     CDS             complement(30321..31187)
                     /gene="ydiB"
                     /locus_tag="RH033_001311"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HDV2990526.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(31199..32464)
                     /locus_tag="RH033_001312"
     CDS             complement(31199..32464)
                     /locus_tag="RH033_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2990527.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYCLAVCVGIANSALDTGGYPALMECFPKATGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFATFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIALDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGEHKFSTRLNTIKHRG"
     gene            complement(32691..33905)
                     /locus_tag="RH033_001313"
     CDS             complement(32691..33905)
                     /locus_tag="RH033_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2990528.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLCRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYSLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKETR
                     HHSLKENVAPGG"
     gene            complement(34005..34361)
                     /locus_tag="RH033_001314"
     CDS             complement(34005..34361)
                     /locus_tag="RH033_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HDV2990529.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKYAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(34497..34604)
                     /gene="rprA"
                     /locus_tag="RH033_001315"
     ncRNA           complement(34497..34604)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="RH033_001315"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(34790..35902)
                     /gene="ydiK"
                     /locus_tag="RH033_001316"
     CDS             complement(34790..35902)
                     /gene="ydiK"
                     /locus_tag="RH033_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HDV2990530.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            36291..39347
                     /locus_tag="RH033_001317"
     CDS             36291..39347
                     /locus_tag="RH033_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDV2990531.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDETLIDERVNALCARLDELIASQQAGVIGWQVCSEL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRVAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALDSQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            39344..39754
                     /gene="menI"
                     /locus_tag="RH033_001318"
     CDS             39344..39754
                     /gene="menI"
                     /locus_tag="RH033_001318"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HDV2990532.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            39854..40042
                     /locus_tag="RH033_001319"
     CDS             39854..40042
                     /locus_tag="RH033_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HDV2990533.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            40591..40959
                     /gene="sufA"
                     /locus_tag="RH033_001320"
     CDS             40591..40959
                     /gene="sufA"
                     /locus_tag="RH033_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HDV2990534.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAVHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            40968..42455
                     /gene="sufB"
                     /locus_tag="RH033_001321"
     CDS             40968..42455
                     /gene="sufB"
                     /locus_tag="RH033_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDV2990535.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYHAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDN
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            42465..43211
                     /gene="sufC"
                     /locus_tag="RH033_001322"
     CDS             42465..43211
                     /gene="sufC"
                     /locus_tag="RH033_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDV2990536.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTSGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            43186..44457
                     /gene="sufD"
                     /locus_tag="RH033_001323"
     CDS             43186..44457
                     /gene="sufD"
                     /locus_tag="RH033_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDV2990537.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGAKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLDGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            44454..45674
                     /gene="sufS"
                     /locus_tag="RH033_001324"
     CDS             44454..45674
                     /gene="sufS"
                     /locus_tag="RH033_001324"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HDV2990538.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            45687..46103
                     /gene="sufE"
                     /locus_tag="RH033_001325"
     CDS             45687..46103
                     /gene="sufE"
                     /locus_tag="RH033_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HDV2990539.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            46252..47256
                     /gene="ldtE"
                     /locus_tag="RH033_001326"
     CDS             46252..47256
                     /gene="ldtE"
                     /locus_tag="RH033_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HDV2990540.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTLVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVSSGATPAASNAPSVESAQNGEPEQGNVLRATQ
                     "
     gene            complement(47319..47555)
                     /gene="lpp"
                     /locus_tag="RH033_001327"
     CDS             complement(47319..47555)
                     /gene="lpp"
                     /locus_tag="RH033_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HDV2990541.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT        12075 a        11464 c        12235 g        11830 t
ORIGIN      
        1 tcatcagcac taaaactcag gctttggctg attacgttta tagcagcata atatgtttct
       61 acagttttat aatgattatg atgaatggct atatgaacag ctgacatatt gtcatgattt
      121 ttagcggcca acaatgtgaa taactgacat tgactaaaag aatatttttt tgcaaaagta
      181 cccagcgttg atatataaga taacacgacg tctttattac ccttgctcat ggcgatgtat
      241 aaagcagggg ttccatatgc tgttgcgcct tttaataaat ccatgatgtt aattttgctg
      301 aggttatatt taacagcgat tccataaact ttagagagga accgtgtgac acacaaaggg
      361 tgattatttt ccattgcggc atataatcct ggctcaccat cacgtgtttt cattgctaaa
      421 atatccatca ccattttttt gcttaaaagc tcttgtgtta ttggagtggt taatgcattc
      481 agaaagatat cgattatttc atcgaatcca tacaacatgg agatatagag tccaggtgtt
      541 tcgttagcac ttttagtttg cagtaattta accagattat cttcatgaat gatatgatta
      601 tctaccagtg actgaatttc ttgcataaat atttttacgt tgtcagtatg tccgttcatc
      661 atagccagaa ataaagcagg tgtaccaacg ctattctgcg cagttattat ttcaatgatt
      721 tctttttcag ggagtatggg taactgtttg aataatgcaa taagctgttg agtctggcct
      781 gtggccatta ttaacaacat tgccgatgga tgtatagggg caaatggcat tcgtgatgca
      841 atacccgcga aaatagcccc tggaactccc tcaggtaata ggggcagaaa agcaattgca
      901 cagttgcgaa catacctatc agcccaggtc tgagcatcgg gacctgaact gatgaattta
      961 tcgagcgaga gaaagccccg tgctgtcctg tgggtgcctc tgacggcaac gttggtatcg
     1021 ttcgggtcgt aaacggacac aacataattt aattgcccat acgctgactg ctttattcgt
     1081 agccttactg acaaagtatg attgtctata tataaaatcg cggacatatg actgacattg
     1141 tttgccatca ttcgacagaa gaaaatatct aaagcgaagc caaaatcctt actcgcgata
     1201 acgcgactcc ctgcaggagg atggcgaagt aatgatctga actccgcgtc ccggactaag
     1261 ggaacatgct gctgtatgga ttcaacggag gagtaatgtt taatcagatt gactttgcct
     1321 tttccatttc tcagtgaatc aagtgcgtat tgcaatgaca gatggcgaca aaaaatagcg
     1381 tcagaaggag attgcgaaaa aaatgcacta ccattgagat ttactgttgg ttgtatagtt
     1441 ttttctctat ggattttttt agacagtttc atacaatggt tacgattttc ggcagttaac
     1501 tgttcctgtt caatataatc cagtatgctt cgcaaatggt tagctttaat aggcgtggtt
     1561 tccagtaata aggaaatgat ttctttttta tagtgtaatg taatgagtat attttctgca
     1621 tggatgggtg aagtggcaaa gttaatggta aaaagggcag tggaataaca attttgtgat
     1681 atttttcgac agtttgcggt aaattcctga gtaacacagt atgacagcat cgaacttaag
     1741 tcagaggtat caagtagggt gatgagttgg cgcgcaaatt gattaatagg ctctgatgaa
     1801 ggcgttggta tgtcgctatc gatgtgcatt ctatgaggag acttataatg agttctgata
     1861 ataatgtcgt tttgactcat aatctattcg ccttatatat gtctggaggt tttgaggatg
     1921 atgattaatt attatcgcca gaacgaaatg tgcacgaaaa attgctataa gaattgtgtc
     1981 acttttatca ttgtgatatg accagttcgt cattaatctc atgatcgttt aaatactacc
     2041 gccaaatcat gcaacgaact ttgctggatt cagtttatgc tgctgtaaat ccgctcgcag
     2101 taaacctttc agacgctccg tgatgttatc agtggttctt taagcgtttt gctggtgtac
     2161 tcactacaaa cgaattgcga atcgaatcaa tgtgaaacgg aaaggtacaa tctccctttt
     2221 tttagtgtta acatcgctca accgggtgga aggtgagagg ttaatgtctt tcatttgtgt
     2281 acctgattgc gaaccaattt agcatttgtt ggctagatgg tttcgcaatg aactgttaat
     2341 aaacaaattt ttctttgtat gtgatctttc gtgtgggtca ccactgcaaa taaggatata
     2401 aaatgcctgt tataactctt cctgatggca gccaacgcca ttacgaccac gctgtaagcc
     2461 ccatggatgt tgcgctggac attggtccag gtctggcgaa agcctgtatc gcagggcgcg
     2521 ttaatggtga actggttgat gcttgcgatc tgattgaaaa cgacgcacaa ctgtcgatca
     2581 ttaccgccaa agacgaagaa ggtctggaga tcattcgtca ctcctgtgcg cacctgttag
     2641 ggcacgcgat taaacaactt tggccgcata ccaaaatggc aatcggcccg gttattgaca
     2701 acggttttta ttacgacgtt gatcttgacc gcacgttaac ccaggaagat gtcgaagcac
     2761 tcgagaagcg gatgcatgag cttgctgaga aaaactacga cgtcattaag aagaaagtca
     2821 gctggcacga agcgcgtgaa actttcgcca accgtgggga gagctacaaa gtctccattc
     2881 ttgacgaaaa catcgcccat gatgacaagc caggtctgta cttccatgaa gaatatgtcg
     2941 atatgtgccg cggtccgcac gtaccgaaca tgcgtttctg ccatcatttc aaactaatga
     3001 aaacggcagg ggcctactgg cgcggcgaca gcaacaacaa aatgttgcaa cgtatttatg
     3061 gtactgcatg ggcagataaa aaagccctca acgcttacct gcagcgtctg gaagaagccg
     3121 ctaaacgcga ccaccgtaaa atcggtaaac agctcgacct gtaccatatg caggaagagg
     3181 cgccaggaat ggtgttctgg cataacgacg gctggacaat cttccgtgaa ctggaagtgt
     3241 tcgttcgtac caaactgaaa gagtaccagt atcaggaagt taaaggtccg tttatgatgg
     3301 accgtgtcct gtgggaaaaa accggtcact gggaaaacta caaagatgcg atgttcacca
     3361 cctcttctga gaaccgtgag tattgcatta agccaatgaa ctgtccgggt cacgtgcaga
     3421 ttttcaacca ggggctgaag tcttatcgcg acctgccgct gcgtatggcc gagttcggca
     3481 gctgccatcg taatgagccg tcaggttcgc tgcatggcct gatgcgtgtg cgtggtttta
     3541 cccaggatga cgcacatatc ttctgtactg aagaacaaat tcgcgatgaa gttaacggat
     3601 gtatccgttt agtctatgat atgtacagca cttttggctt cgagaagatc gtcgtcaaac
     3661 tctccactcg tcctgaaaaa cgtattggca gtgacgaaat gtgggatcgt gctgaggcgg
     3721 acctggcggt tgcgctggaa gaaaacaaca tcccgtttga atatcaactg ggtgaaggcg
     3781 ctttctacgg tccgaaaatt gaatttaccc tgtatgactg cctcgatcgt gcatggcagt
     3841 gcggtacagt acagctggac ttctccttgc cgtctcgtct gagcgcctcc tatgtgggcg
     3901 aagacaacga gcgtaaggta ccggtaatga ttcaccgcgc aattctgggg tcgatggaac
     3961 gattcatcgg tatcctgacc gaagagtttg ctggtttctt cccgacctgg cttgcgccag
     4021 ttcaggttgt tatcatgaac attaccgatt ctcagtctga atacgttaac gaattgacgc
     4081 aaaaactatc aaatgcgggc attcgtgtta aagcagactt gagaaatgag aagattggct
     4141 ttaaaatccg cgagcacact ttgcgtcgcg tcccatatat gctggtctgt ggtgataaag
     4201 aggtggaatc tggcaaagtt gccgttcgca cccgccgtgg taaagacctg ggaagcatgg
     4261 acgtaaatga agtgatcgag aagctgcaac aagagattcg cagccgcagt cttaaacaat
     4321 tggaggaata aggtattaaa ggcggaaaac gagttcaaac ggcgcgccct aaccgtatca
     4381 atggcgaaat tcgcgcccag gaagttcgct taacaggtct ggaaggcgag cagcttggta
     4441 ttgtgagtct gagagaagct ctggagaaag cagaagaagc cggagtagac ttagtcgaga
     4501 tcagccctaa cgccgagccg ccggtttgtc gtataatgga ttacggcaaa ttcctctatg
     4561 aaaagagcaa gtcttctaag gaacagaaga aaaagcaaaa agttatccag gttaaggaaa
     4621 ttaaattccg tcctggtaca gatgaaggcg actatcaggt aaaactccgc agcctgattc
     4681 gctttctcga agagggtgat aaagccaaaa tcacgctgcg tttccgcggt cgtgagatgg
     4741 cgcaccagca aatcggtatg gaagtgctta atcgcgtgaa agacgatttg caagaactgg
     4801 cagtagtcga atccttccca acgaagatcg aaggccgcca gatgatcatg gtgctcgctc
     4861 ctaagaagaa acagtaaggc cttcaagtag cactgtctgt ggagccttcg ggcttcgcag
     4921 actttgttcg cctttgtttc tgtttattaa caatgcgaag tggaagttat taaaatgcca
     4981 aaaattaaga ccgtacgcgg tgctgctaag cgcttcaaaa aaaccggtaa aggtggtttt
     5041 aagcacaagc acgctaacct gcgtcacatt ctgaccaaaa aagcgaccaa acgtaaacgt
     5101 cacctgcgtc cgaaagccat ggtttccaaa ggcgatctgg gcctggtaat cgcgtgcctg
     5161 ccgtacgcat aagccgttaa cgttttttaa ctttttaatt agaatataga tacaggagag
     5221 cacatatggc tcgcgtaaaa cgtggtgtta ttgcacgcgc acgtcacaag aaaattttga
     5281 aacaagctaa aggctactac ggtgcgcgtt ctcgcgtata ccgcgttgcc ttccaggctg
     5341 ttatcaaagc tggtcagtat gcttaccgcg accgtcgtca acgtaagcgt cagttccgtc
     5401 aactgtggat tgcgcgtatc aacgcagcag cacgtcagaa cggtatttct tacagcaaat
     5461 tcatcaatgg cctgaaaaaa gcctctgttg aaatcgaccg taagatcctg gctgatatcg
     5521 cagtattcga caaagtagcg ttcaccgctc tggttgaaaa agcgaaagca gctctggcat
     5581 aagccagttg aaagagggag ctagtctccc tcttttcgtt tcaacgccat caaaacattg
     5641 acttttatcg ccgtagcctt ttcaataaag gtcttttgaa gagtaccaaa aggtaacgca
     5701 agcaatgaat gctgctattt tccgcttctt tttttacttt agcacctgac tccaggaggc
     5761 tagcgcgtga gaagagaaac ggaaaacagc gcctgaaagc ctcccagtgg aggctttttt
     5821 tgtatgcgcg ttttgaaaaa taaaaagcct gtctcatcag gcgtaattgt cgcagccagt
     5881 ctggacacgg ccagcgcgga gaaaccggag cgtacttaag tacgtgagga tttcgagcac
     5941 tgcccgggac caaaatggca agtaaaatag cctgatggga tgggctctaa gtccaacgaa
     6001 ccagtgtcac cactgacaca atgaggaaaa acatgtcaca tctcgcagaa ctggttgcca
     6061 gtgcgaaggc ggccattagc caggcgtcag atgttgccgc gttagataat gtgcgcgtcg
     6121 aatatttggg taaaaaaggg cacttaaccc ttcagatgac gaccctgcgt gagctgccgc
     6181 cagaagagcg tccggcagct ggtgcggtta tcaacgaagc gaaagagcag gttcagcagg
     6241 cgctgaatgc gcgtaaagcg gaactggaaa gcgctgcact gaatgcgcgt ctggcggcgg
     6301 aaacgattga tgtctccctg ccgggtcgtc gcattgaaaa cggcgggctg catccggtga
     6361 cccgtaccat cgaccgtatc gaaagtttct tcggtgagct tggctttacc gtggcaaccg
     6421 ggccggaaat cgaagacgat tatcataact tcgatgctct gaacattcct ggtcaccacc
     6481 cggcgcgcgc tgaccacgac actttctggt ttgacgctac ccgcctgctg cgtacccaga
     6541 cctctggcgt acagatccgc accatgaaag cccagcagcc accgattcgt atcatcgcgc
     6601 ctggccgcgt ttatcgtaac gactacgacc agactcacac gccgatgttc catcagatgg
     6661 aaggtctgat tgttgatacc aacatcagct ttaccaacct gaaaggcacg ctgcacgact
     6721 tcctgcgtaa cttctttgag gaagatttgc agatccgctt ccgtccttcc tacttcccgt
     6781 ttaccgaacc ttctgcagaa gtggacgtca tgggtaaaaa cggtaaatgg ctggaagtac
     6841 tgggctgcgg gatggtgcat ccgaacgtgt tgcgtaacgt tggcatcgac ccggaagttt
     6901 actctggttt cgccttcggg atggggatgg agcgtctgac tatgttgcgt tacggcgtca
     6961 ccgacctgcg ttcattcttt gaaaacgatc tgcgtttcct caaacagttt aaataaggca
     7021 ggaatagatt atgaaattca gtgaactgtg gttacgcgaa tgggtgaacc cggcgattga
     7081 tagcgacgcg ctggcaaatc aaatcactat ggcgggcctg gaagttgacg gtgtagaacc
     7141 ggttgccggt agcttccacg gcgtggttgt tggtgaagtg gttgagtgtg cgcagcatcc
     7201 gaacgctgac aaactgcgtg tgacaaaagt gaatgtcggc ggcgatcgcc tgctggatat
     7261 cgtctgcggt gcgccaaact gccgtcaggg cctgcgtgtg gcggtagcga ccattggtgc
     7321 tgttctgccg ggcgatttca aaattaaagc ggcgaaactg cgtggcgaac cgtctgaagg
     7381 gatgctgtgc tccttttctg agctgggaat ttctgacgat cacagcggca ttatcgaact
     7441 gcctgcggat gcgccgattg gcaccgacat ccgcgaatac ctgaaactcg atgacaacac
     7501 catcgaaatc agcgtaacgc caaaccgtgc cgattgctta ggcattatcg gcgtggcgcg
     7561 tgatgttgcc gtgctgaacc agctgccgct ggttgaaccg gaaatcgttc cggttggtgc
     7621 gaccatcgac gacacgctgc cgattgcagt cgaagcgccg gaagcctgcc cgcgctatct
     7681 tggccgtgtg gtaaaaggca ttaacgttaa agcgccaact ccgctgtgga tgaaagaaaa
     7741 actgcgtcgt tgcgggatcc gttctatcga tgcagttgtt gacgtcacca actatgtact
     7801 gcttgaactg ggccagccga tgcacgcctt cgacaaagat cgcattgaag gcggcattgt
     7861 ggtgcggatg gcgaaagagg gcgaaacgct ggtattgctc gacggtaccg aagcgatgct
     7921 gaacgctgac actctggtca tcgccgacca caacaaggcg ctggcgatgg gcggcatctt
     7981 tggtggcgaa cactctggcg tgaatgacga aacacaaaac gttctgctgg aatgcgcttt
     8041 cttcagccct ctgtctatca ccggtcgtgc tcgtcgtcat ggcctgcata ctgatgcgtc
     8101 tcaccgttat gagcgtggcg ttgatccggc actgcagcac aaagcgatgg aacgtgcgac
     8161 ccgtctgctg atcgacatct gcggtggtga ggctggtccg gtaattgata tcaccaacga
     8221 agcaacgctg ccgaagcgtg caaccatcac tttgcgtcgc agcaaactgg atcgcctgat
     8281 tggccatcat attgccgatg agcaggtcac tgacattctg cgtcgtctcg gctgcgaagt
     8341 gaccgaaggc aaagacgagt ggcaggcagt tgcgccgagc tggcgtttcg acatggagat
     8401 tgaagaagat ctggtcgaag aagtcgcgcg tgtttacggc tacaacaaca tcccggatga
     8461 gccggtacag gcaagcctga ttatgggtac tcaccgtgaa gctgacctgt cgctcaagcg
     8521 cgtgaaaacg ctgctcaacg acaaaggcta tcaggaagtg atcacctata gcttcgttga
     8581 tccgaaagtg cagcagatga tccatccagg cgttgaagcc ttactgctgc caagcccgat
     8641 ttccgttgaa atgtcagcaa tgcgtctttc tctgtggacc ggcctgctgg caactgtggt
     8701 gtacaaccag aaccgtcagc agaaccgtgt acgcattttc gaaagcggtc tgcgtttcgt
     8761 gccagatact caggcaccgt tgggcattcg tcaggatctg atgttagccg gtgtgatttg
     8821 cggtaaccgt tacgaagagc actggaacct ggcaaaagag accgttgatt tctatgattt
     8881 gaaaggcgat cttgaatccg ttctcgacct gaccggtaaa ctgaatgagg ttgagttccg
     8941 tgcagaagcg aatccggcac tgcatccggg gcaatccgca gcgatttatc tgaaaggtga
     9001 acgtattggt tttgttgggg ttgttcatcc tgaactggaa cgtaaactgg atcttaacgg
     9061 tcgcactctg gtgttcgaac tggagtggaa caagctcgca gaccgcgtgg tgcctcaggc
     9121 gcgcgagatt tctcgcttcc cggcgaaccg tcgtgacatc gcggtggtgg tcgcagaaaa
     9181 cgttcccgca gcggatattt tatccgaatg taagaaagtt ggcgtaaatc aggtagttgg
     9241 cgtaaactta tttgacgtgt accgcggtaa gggtgttgcg gaggggtata agagcctcgc
     9301 cataagcctg atcctgcaag ataccagccg tacactcgaa gaagaggaga ttgccgctac
     9361 cgtcgccaaa tgtgtagagg cattaaaaga gcgattccag gcatcattga gggattgaac
     9421 ctatggcgct tacaaaagct gaaatgtcag aatatctgtt tgataagctt gggcttagca
     9481 agcgggatgc caaagaactg gttgaactgt ttttcgaaga gatccgtcgc gctctggaaa
     9541 acggcgagca ggtgaaactc tctggttttg gtaacttcga tctgcgtgat aagaatcaac
     9601 gcccgggacg taacccgaaa acgggcgaag atattcccat tacagcacgg cgtgtggtga
     9661 ctttcagacc cgggcagaag ttaaaaagcc gggtcgaaaa cgcttcgccc aaagacgagt
     9721 aatctgatct aactaaaaag gccgctctgc ggcctttttt cttttcactg tcgaagagtc
     9781 accgtaaaat caacgccatg acacttcagc agaacggata ccatgctgac acttgcccgc
     9841 caacaacagc gacaaaatat tcgctggtta ttatgcctgt cagttttgat gctgctggcg
     9901 cttctcttaa gcctttgcgc cggtgaacaa tggatctcgc caggtgactg gttttctcct
     9961 cgaggcgaac tgttcgtctg gcagattcgc ctgccacgta cgctggctgt attgctggtt
    10021 ggtgcggcgt tggctatatc cggcgctgta atgcaggcgt tatttgaaaa tcctctggca
    10081 gaacctggac tacttggcgt ctctaatggc gcaggcgtgg ggcttatcgc cgcggtattg
    10141 cttgggcaag ggcaactccc caactgggcg ctggggctgt gtgcgattgc tggcgcgctt
    10201 atcatcactt taatactctt acgtttcgcc cgtcgtcatc tttcgaccag tcggttattg
    10261 ctggctggcg ttgcattagg gattatctgt agcgcactaa tgacgtgggc tatctacttt
    10321 tccacctctg ttgatttacg tcagctgatg tactggatga tgggcggttt tggcggcgta
    10381 gactggcggc aaagctggct gatgctggca ttgatccccg tgttgttgtg gatctgttgt
    10441 cagtccaggc cgatgaatat gttagcactt ggcgagatct cggcgcggca actgggttta
    10501 cccctgtggt tctggcgcaa tgtgctggtg gcagcgaccg gctggatggt tggcgtcagt
    10561 gtggcgctgg cgggtgctat cggctttatt ggtctggtga tcccccatat tctccggtta
    10621 tgtggtttaa ccgatcatcg cgtattactt cccggctgcg cgctggcagg ggcgagcgca
    10681 ttgctgctgg ctgatgttgt agcgcgcctg gcattagctg ccgcagagct gcctattggc
    10741 gtggtcaccg caacgttggg tgcgccggtg tttatctggt tattgttaaa agcaggacgt
    10801 tagccgcaaa aagacggtct atgattaaaa gctaagattt ttactgacca cacccaggag
    10861 aaacgatgca agattccatt ctgacgaccg tagtgaaaga tatcgacggt gaagtgacca
    10921 cgctggagaa gtacgccggt aatgtgctgt tgattgtcaa tgtcgcctca aagtgtggct
    10981 taacgccgca atatgagcag ttggagaata ttcagaaagc ctgggccgat cgtggttttg
    11041 tggtgctggg attcccgtgc aaccagtttc tggagcaaga accgggcagc gatgaagaga
    11101 ttaaaactta ctgcaccact acatgggggg tgacgttccc gatgttcagt aagattgaag
    11161 ttaatggtga aggacgccat ccgctgtatc aaaaattgat tgccgcagcg ccgaccgcag
    11221 tcgcgccgga agagagcgga ttctatgccc gtatggtcag caaaggtcgt gcaccgctgt
    11281 accctgatga tatcttatgg aattttgaga aatttttagt tggcagggac ggattggtca
    11341 tccagcgttt ttccccggat atgacgccgg aagatcccat tgtgatggaa agcattaaac
    11401 tggcgttggc aaaataatgt ctattgtgat gcagttacag gatgttgcgg agtctacccg
    11461 cctggggccg ctttctggcg aggttcgggc tggggagatc ctgcacctgg tggggccgaa
    11521 tggcgcgggt aagagtacct tactggcgcg aatggccgga atgaccagcg gtaagggaag
    11581 cattcagttc gcggggcaac cactggaagc atggtccgca acaaaactcg cgctgcatcg
    11641 cgcctatctt tcacaacaac agacgccacc gtttgcaatg ccggtctggc actacctgac
    11701 actgcatcag cacgataaaa cgcgtaccga actactgaat gatgtcgcag gggcgctggc
    11761 tcttgatgac aaactcggac gtagcaccaa tcaactttcc ggcggcgaat ggcaacgcgt
    11821 acgtcttgct gcggtggtgc tgcaaatcac accacaagcc aatcccgcag gccaattgct
    11881 gcttcttgat gagccgatga acagtcttga tgttgcgcaa caaagtgcat tagacaaaat
    11941 tctgagcgcg ctgtgtcagc aaggactggc gattgtgatg agcagtcacg atctcaacca
    12001 tacattgcgt catgcgcatc gggcgtggtt gctaaaaggt ggaaaaatgc tggccagtgg
    12061 acgcagggaa gaggtgctca cgccgccaaa tctggcgcag gcctatggga tgaattttcg
    12121 ccgtctggat atcgaaggtc acagaatgct gatttcgacc atctgatttc attagtatct
    12181 tgaaaatcgg gtaattacga cgctaaatta atgcaagaat aaaaaacaga ggaaccgtac
    12241 ggaatgcgtt tctgccttat tttgatcaca gcactgtttc tggccgggtg tagccaccat
    12301 aaagcaccgc cgccaaatgc cagactttct gattcgatta ccgttattgc cggtttgaac
    12361 gaccagctac aaagctggca tggcacgccg tatcgttatg gtggaatgac gcggcgcggt
    12421 gtggactgtt cgggatttgt ggttgtgacg atgcgcgatc gtttcgattt gcagctgccc
    12481 cgtgaaacca aacaacaagc ctctatcggc acgcaaattg ataaagacga gttgctgcca
    12541 ggtgacctgg tctttttcaa aacgggctcc ggacaaaatg gtttgcatgt aggtatttac
    12601 gataccaaca accaatttat ccacgcctct accagcaagg gagtgatgcg ttcctcactt
    12661 gataatgtct attggcagaa aaatttctgg caggcgagac gaatctagta tgtcgaaaaa
    12721 ttgacggcga tgatacatcg ccgtcaattt actccagaat acggtagtat tttgcagaca
    12781 aaaattccgt aaatggatta ttcgtttata atcaggataa tctaaaaaaa ggacccctga
    12841 aaaagtggtt aacatggaac aaatgaaagt tactccttct gtaattgtta tgtcttatgg
    12901 cctcatccct tttagccgga tactgaaaaa catccttcga gagggacggt taccatgaag
    12961 atttttttgg agaatcttta tcattctgat tgctatttcc tgccgataag agataaccag
    13021 caagaccttg tcggcgttga gttaattacc catttctcca gtgaagatgg tacggttcgt
    13081 atcccgacga gtcgggttat agcgcaactt accgaggagc aacattggca attattttca
    13141 gagcagttgg aattactaaa atcatgccaa cactttttta ttcaacataa actctttgcc
    13201 tggcttaatt taaccccaca agtggctact ttattattag acagggataa ttacgcagac
    13261 gaactattaa aatatccgtt tattgaactg ttgattaatg agaattaccc gcatctcgat
    13321 gaaggaaaag ataatcgcga tcttttttcc ttatcgcaaa tgtatcccct tgtgttaggt
    13381 aatttgggcg caggcaatag cacaatgaaa gcggtttttg atggtctttt cacccgcgta
    13441 atgctggata aaagttttat tcagcaacaa atcacgcatc gctccttcga accgtttatt
    13501 cgtgcgattc aggcgcaaat ttccccttgt tgcaattgca tcatcgcggg gggcattgat
    13561 acgccagaaa tattagcaca gattaccccc ttcgatttcc atgctctcca gggatgtctg
    13621 tggcctgccg ttcccataaa tcagataacg acgttggttc agcgataacc ctcctgtttg
    13681 ctggtgttta agacgagagt aaccgtctac actatcaaac aggaggatct atgaccctgt
    13741 cttttgttac ccgctggcgc gatgaattgc cagaaaccta tacagcactt tcccctacgc
    13801 ctttaaataa tgcccggctg atttggcata ataccgaact cgctaacacg ctgagtattc
    13861 catcgtcgct gtttaaaaat ggcgcaggtg tctggggcgg cgaaacccta ctgcctggta
    13921 tgtcaccact ggcgcaggtt tacagtggtc atcagttcgg cgtctgggcg ggccaactgg
    13981 gtgatgggcg cggcatttta ctcggcgaac aacggcttgc tgatggcact acaatggact
    14041 ggcatctgaa aggtgctggc ctgacgcctt attcgcgaat gggtgatgga cgggcggttt
    14101 tacgttcgac gatacgagaa agtctcgcca gtgaggcgat gcattatctg ggcattccga
    14161 cgacccgcgc gttaagtatc gtcaccagcg attcgccagt gtatcgggaa acggtggagc
    14221 caggcgcgat gctgatgcgt gtggcaccaa gtcatctgcg ctttggtcat ttcgaacatt
    14281 tttactatcg ccgcgagccg gaaaaggttc gtcagttagc tgactttgcc attcgtcatt
    14341 actggtcaca tcttgcagat gatgaggaca aataccgtct ctggtttagc gatgttgtcg
    14401 cacgtaccgc atcgctaatt gcccaatggc agacggtcgg ctttgctcat ggggtgatga
    14461 ataccgacaa catgtcgctg ctgggactga cgcttgatta cgggcctttt ggttttcttg
    14521 atgattacga acccggtttt atttgtaatc actcggatca tcaagggcgt tatagctttg
    14581 ataatcaacc tgctgtcgcg ttgtggaatt tacagcgtct ggcgcagaca ttgtcaccat
    14641 ttgtcgccgt agatgccctg aatgaggccc tggacagtta tcagcaggtt ttgttgacgc
    14701 attatggaca acggatgcgg cagaaactgg gcttcatgac ggagcaaaaa gaggataacg
    14761 cgctactgaa tgaattattc agtctgatgg cgcgagagcg cagcgattat acccgcacat
    14821 tccgcatgct gagtctgacc gagcagcaca gcgcggcgtc accgctacgt gatgagttta
    14881 ttgatcgtgc ggcatttgat gactggtttg cccgttatcg ggtacgtttg caacaagacg
    14941 aggttagcga tagtgagcgt cagcagctga tgcaaagtgt taacccagct ctggtgttgc
    15001 gcaactggtt ggcgcaacgg gcgattgaag cggcagaaaa gggtgatatg acggaattgc
    15061 accgcctgca tgaggcgttg cgaaatcctt tcagcgacag agatgatgac tatgtcagtc
    15121 gtccacctga ctggggtaaa cggctggaag tcagctgctc gagttaaagg ctacttggtc
    15181 aacagcagct tgccagcctg agttttacgg agcagatatt cttgcccgtc atgatcgata
    15241 atcagtttgc catccggacc taacagtgtc tggctggtaa ttcgccggat gggctgcggg
    15301 gaagcggtct tgtgattggc atccgtttca ggcgtgagtt ttctgctatc cgtataacgc
    15361 ataaaaatta ataataattt atataacaat gattcttatt atcaatatta tggcgggcca
    15421 gagcaagcag taatttgttg agaagacaac aagttgcgca gaaatgccca tcccgtcagg
    15481 aatgggcacg cattcagaag cgagtatcta ccgcagaggc gagtttttcg accaggcgtt
    15541 cggtatcttc ccagcccaga cacgggtcgg taatggattg accgtaagtg agcggctgac
    15601 cgccgacgat tttttgcgtt ccttcgcgca ggaaactttc cgccataatt ccagcaatcg
    15661 ccgtagatcc attgcggatt tgctgacaaa tatcctcaca aacttctaac tggcgtcggt
    15721 gctgcttctg gcagttaccg tggctgaaat ccaccaccag atgttcaggt aaatcaaact
    15781 cgtgcagcgt atcgcaggct gcggcgatat catcggcatg ataattcggt tttttgccgc
    15841 cacgcataat aatgtggcca tacgggttgc cgctggtctg atagatggtc atctgaccat
    15901 ttttgtctgg cgagaggaac atatggctgg cgcgggctgc gcggatagca tccacggcaa
    15961 tccgcgtgtt gccatcggta ccatttttaa aacctaccgg acaggagagt gccgaagcca
    16021 tttcacggtg gatctgactt tcggtagtac gtgcgccaat cgcgccccaa ctgattaaat
    16081 cagcaataaa ctgaccggtc accatatcga ggaactcagt cgcggtaggg acgcccagct
    16141 catttacctg taaaagtaat ttgcgcgcca gctccagacc gtgatttacc cgatagctgc
    16201 cgtttaaatc tggatcggag attagccctt tccagccgac aacagttcgt ggtttttcaa
    16261 aataggtgcg cattacgatt tccagccgtg actggtactg gttgcgcagc gactgcagac
    16321 gggtggcgta ctccattgca gcggtgagat cgtggatcga gcaggggcca ataatgacca
    16381 acagtcgctt atcttcacca ttcagtattt tttcaattct gcggcgggag tcggtgacat
    16441 gggtggcgac gccaggcgtt acgggatacc gtagcgcgag ttcggcgggc gttaccaggc
    16501 tctcaatacg cgcagtacgg agttcgtcag ttctgttcat tacaagtctc agaaatttgt
    16561 tgcttctacg gcaggactac cgggaagtga tgcgcatcac gataaaccaa tcgcagctga
    16621 tttcaagtgc gaggtgtgtc acacctcgcc gggtggttag catgataaca aaaaaacaag
    16681 ctaatgcact agttctctag tacattcgac ggctaaggcc catgatatcg aggatcttgg
    16741 tggcaatctc ttctaccgaa taattggtac tgttaatcca cgggatctga tttttacggt
    16801 acaacgcttc cacttccgcg acttccatcc tgcactgacg aagcgaggca tagcgactgt
    16861 tctcccgacg ttcctcgcga atcgccgcca gacgttccgg gtcgatagtc aggccgaaca
    16921 atttatgctg aagcggtttg agcgacgcgg gtagcacaag attatccata tcgtcggcaa
    16981 taaaggggta gtttgccgcg cggataccaa actgcattgc cagatacaga ctggtggggg
    17041 ttttaccaca gcgagaaacg ccgagcagga tcacctgagc ctggtccaga ttgcgtaacg
    17101 aaatgccatc atcgtgggcg agggtgtaat caatcgcagc aatgcgcgca tcatatttat
    17161 tgagattatt agggttaagg ccatgggtac gatgagcaat cggcgttgga tccagtttca
    17221 tctcttgttg tagcggggca accagcgcct gaacgatatc ctggcaaaag ccctcacttt
    17281 gcaagatgat ggcgcgaatc tccggcaaca cgatggagta gaagaccagc gggcgcacac
    17341 ctgtctggtg ataaattgcg tcaatctgat ccttcactgc ccgtgcacgg ctctcatttt
    17401 cgacaaacgg cagcgtaatg ctgctgatag tgacgggaaa ttgtgacatt actgcgtgtc
    17461 ctaatacctc cgcagttatt gccgtaccat cagaaatata aaaaacgtgg cgatcaacag
    17521 cattatccat tttgttcttc ccgtgatgca gacataattc tatgaaagca taaattaaaa
    17581 acgcacagaa gcgtagaacg ttatgtctgg tttataaaat gaaccttcaa ttttattttt
    17641 tatgaaaaca gcatttcatt tttacggttt cgtttatacc gatggtttat gtggaaattg
    17701 tcgaagagag cagatttgcg caacgctggg atcagtctta aaaagtaaaa aaatatattt
    17761 gcttgaacga ttcaccgttt ttttcatccg gttaaatatg caaagataaa tgcgcagaaa
    17821 tgtgtttctc aaaccgttca tttatcacaa aaggattgtt cgatgtccaa caatggctcg
    17881 tcaccgctgg tgctttggta taaccaactc ggcatgaatg atgtagacag ggttgggggc
    17941 aaaaatgcct ccctgggtga aatgattact aacctttccg gaatgggtgt ttccgttccg
    18001 aatggtttcg ccacaaccgc cgacgcgttt aaccagtttc tggaccaaag cggcgtaaac
    18061 cagcgcattt atgaactgct ggataaaacg gatattgacg atgttaccca gcttgcgaaa
    18121 gcgggcgcgc aaatccgcca gtggattatc gacactccct tccagcctga gctggaaaac
    18181 gccatccgcg aagcctatgc acagctttcc gccgatgacg aaaacgcctc gtttgcggtg
    18241 cgttcctccg ctactgcaga agatatgccg gacgcttctt ttgccggtca gcaggaaact
    18301 ttcctcaacg ttcagggttt tgacgccgtt ctcgtggctg tgaaacatgt atttgcttct
    18361 ctgtttaacg atcgcgccat ctcttatcgt gtgcaccagg gttacgatca ccgtggcgtg
    18421 gcgctgtccg ccggtgttca gcggatggtg cgctccgacc tcgcatcttc tggcgtgatg
    18481 ttctccattg ataccgaatc cggctttgac caggtggtgt ttatcacttc cgcatggggc
    18541 cttggtgaaa tggtcgtgca gggtgcggtt aacccggatg agttttacgt gcacaaaccg
    18601 acactggcgg cgaatcgccc ggctatcgtg cgccgcacca tggggtcgaa aaaaatccgc
    18661 atggtttacg cgccgaccca ggagcacggc aagcaggtta aaatcgagga cgtaccgcaa
    18721 gaacagcgtg acatcttctc gctgaccaac gaagaagtgc aggaactggc aaaacaggcc
    18781 gtacaaattg agaaacacta cggtcgccca atggatattg agtgggcgaa agatggccac
    18841 accggtaaac tgttcattgt gcaggcgcgt ccggaaaccg tgcgctcacg cggtcaggtc
    18901 atggagcgtt atacgctgca ttcacagggt aagattatcg ccgaaggccg tgctatcggt
    18961 catcgcatcg gtgcgggtcc ggtgaaagtc atccatgaca tcagcgaaat gaaccgcatc
    19021 gaacctggag acgtgctggt cactgacatg accgacccgg actgggaacc gatcatgaag
    19081 aaagcatctg ccatcgtcac caaccgtggc ggtcgtacct gtcacgcggc gatcatcgct
    19141 cgtgaactcg gcattccggc ggtagtgggc tgtggtgatg caacagaacg gatgaaagac
    19201 ggtgagaacg tcactgtttc ttgtgccgaa ggtgataccg gttacgtcta tgcggagttg
    19261 ctggaattta gcgtgaaaag ctccagcgta gaaacgatgc ctgatctgcc gttgaaggtg
    19321 atgatgaacg tcggtaaccc ggaccgtgct ttcgacttcg cctgcctgcc gaacgaaggc
    19381 gtcggccttg cgcgtctgga atttatcatc aaccgtatga ttggcgtcca cccacgcgca
    19441 ctgcttgagt ttgacgatca ggaaccgcag ttgcaaaacg aaatccgcga gatgatgaaa
    19501 ggttttgatt ctccgcgtga attttacgtt gggcgtctga ctgaagggat cgcgacgctg
    19561 ggtgccgcgt tttatccgaa gcgcgtcatt gtccgtctct ctgattttaa atcgaacgaa
    19621 tatgccaacc tggtcggtgg tgagcgttac gagccagatg aagagaaccc gatgctcggc
    19681 ttccgtggcg cgggccgcta tgtttccgac agcttccgcg actgcttcgc gctggagtgt
    19741 gaagcagtga aacgtgtgcg caacgacatg gggctgacca acgttgagat catgatcccg
    19801 ttcgtgcgta ccgtagatca ggcgaaagcg gtggttgagg aactggcgcg tcaggggctg
    19861 aaacgtggtg agaacgggct gaaaatcatc atgatgtgtg aaattccgtc caacgccttg
    19921 ctggcagagc agttcctcga atatttcgac ggcttctcaa ttggctcaaa cgacatgaca
    19981 cagctggcgc tcggtctgga ccgtgactcc ggagtggtgt ctgaactgtt cgatgagcgc
    20041 aacgatgcgg tgaaagcact gctgtcgatg gcgattcgtg ccgcgaagaa acagggcaaa
    20101 tatgtcggga tttgcggtca gggtccgtcc gaccacgaag actttgccgc atggttgatg
    20161 gaagagggga tcgatagcct gtctctgaac ccggataccg tggtgcaaac ctggttaagc
    20221 ctggctgaac tgaagaaata aaataaatcc ccggcggcgt tttgtcgccg ggtttatgtg
    20281 atccccgaag atgaaactta ttcaatctct tcacagacat cctgagttaa tcgccgcata
    20341 atatctttcc ttaacaaaaa cttttgtatt ttacctgagg ctgtgcgcgg tagtttttcg
    20401 attaccacga tatgttcagg atatttatat ttcgcgaccc gtttacggct aaaaaatgcc
    20461 actacatctt ccagcgataa tgaatgatgc ggtgctttca gcacgacata agcgcatgaa
    20521 cgttcaccta agcgttcatc gggcattgca accacacaag catcgtgaat tttaggatgc
    20581 tgcaataaaa tatcttctac ttcacggctg ctaatatttt cgccgccgcg gacaataata
    20641 tctttcttgc gcccggttat ttttatatag ccagcctcat ccatgcggca gagatcgccg
    20701 ctgtaatacc agccttcttc atccagcgca cgggcggtta attcaggttc atcaaaatac
    20761 cccataaaca cattggggcc acgcgaggct tcttcacctt cgcaacctgg cggtaaagtc
    20821 ttgcgtgcgt catcgaccac tttaatctct acacctgcgg cagcgtaacc atcggtgtgt
    20881 ataaagcgcg acaaaggatc atcgagattc accaccgcat gcggcgaact ttctgtggaa
    20941 ccataaacac ttaataattt aatgccgcgc tgctggcatt cacgcgccac ttttttgggg
    21001 attgtggtac cgccgcaaag aaagaaacgc agcgctgaaa ggtctgcggg ctgtttctcc
    21061 agtaaattca aaagatcata gacaaacggc gttgcgccga gcatacaggt gcaacgctgc
    21121 tgctcaagca gcgcgagaca cgcagcagga gtgaaaatat ctaacaacac gctgcgagca
    21181 ccaatcaaaa aaggtgctgt tacgccatgc agaaagcccg ttgcgtgacc aagtggcgca
    21241 ggcatcataa agacatcctg ccaggtcaga tttagccgcg cgcaataagc ccgctcactg
    21301 gcgagaatat tgttatgcgt tagcatcacg ccctttggca gaccctcggt tccggaggta
    21361 aacagcaccg cggctaattc atcgccgtgg accgttatcg ccgtggtcag aggagtattg
    21421 tcggcgataa tctgacttaa tgagagagca gaggtggcgg gagccagttt gtccacgccg
    21481 acaatttgtt gtagttgtgg aagctgattt tgcagcggca ggattaaatc taccggacgc
    21541 gtttgtttaa acaacgtcgg tgcaaagaac atttttgcct gacacttatt gagcacccac
    21601 accagttctg cttctcgcca ggaaggcaac agcggcacgg aaaccgcacc ggttttcagg
    21661 caggcaagat agataacggt aaattcacac cagcctggca gttgaaatgc gatgcgatcg
    21721 cctgattcaa tacccttcgc taacatccag tttgccagac agctcgcggc gtgatcgagc
    21781 gcgctataag tgtacgttgc accatgattg tcgaccacgg caattttgtc tggcatcgca
    21841 cgagcggtct gctgccagta atcggccagt gaagcatcgc cccataaccc ttgctgacga
    21901 tacgccgcac gacgttgttc gttgaacgtt aatgtcactt tcattttgga ctctcgaaac
    21961 agaacatcag gcccgagatg cgggcctgtg ggatgcatga ttagccgtag cgaaattcaa
    22021 taccgaaggt gcctgcggga tattgccact gttcgagaat agtgttgccg cacagcaccc
    22081 gacaggtgcc gcactccaga caaccggcgg aatcgaaatg aatgtttcct gcgtcatcct
    22141 gcttataaag tccggcaggg caggctttca ttaatttacg gaattcattg atatcgggat
    22201 ttgccgccag aatgatatgc gggtggccct catcaacatg gaatttattt acgcctaatt
    22261 tgatgtcgac gttaaccgta gcgttctggc tcatagcgcg gttgctccct taatgccatc
    22321 tttcagcaag ttgatcagtc caattttctt cgcgtgtccc atgatcattt tgcgtaccgg
    22381 ttggttaggt ttgccgtcga tggtgaacat ctcgttcatg atgtcggcga ccattcgtgg
    22441 gtattggcta aacaggcgcg ggttttccat cagcgccggg atcttgcgaa aatgctgcat
    22501 atcacgcatg acgcagcttt gttccagctc acgtttgtat tgcgcaagac tgctggcgga
    22561 gaaatccgcg cgttctttgg cggcgatcac tgttgtggcg gcagcctgag ccgatgcaat
    22621 ggctaaatcc atgccgcgga ccgtaaaacc caaattcagg cagaagcctg cggcgtcacc
    22681 aacgatcatc acgccatcgt taaccagctg cggcaccatt gccagaccgc cctccggcac
    22741 catatgcgcg gagtattcaa gcagtttgcc gccgctaatc agcgggcgaa tggcggggtg
    22801 ttgtttaaaa tcttccagca tttgcggcac acttttttgc gcatgggcga tatcacccaa
    22861 tccacaaacc agccccaacg atacggaatc tttgttggta tagaggaatc cgccgcccat
    22921 caggccgtca gaaggggaac cggcaaacag ccaggcggca ccttcctcgc ccgtaacatt
    22981 aaagcgatcg ttgatctgtt ctggtgtcag gcctataacc tctttaacac caacagcgta
    23041 atgatgcgga tcggaagcgg aaaccattcc cagcgagcgg ccaagcatcg agttaacgcc
    23101 atcagccaga atcaccacat tcgcttcgag aatatcatcc ccggcctgga cgccagtgac
    23161 cttgtttcct tcacgaacca gtgcatcgac gcgtacgccc gggataaact gcgcgcccgc
    23221 ctgctcggct tgttccatca accacgggtc cagacgatta cgcaatacgg tataagacgc
    23281 gtgttgcgga acatctggtt gctcgcggtg aaaatcgagg gtaacggcgc tttcttcggt
    23341 taagaaggag attttctcgc gtgtgacctt gcgttctacc ggcgctgatg ctgcaaagcc
    23401 tggaataatt gcgtcaagtg tgtgggcata aagacgcccg ccggtcatgt ttttacatcc
    23461 ggcactgtcg ccgcgttcta tcaccaggac atccagcccg gctcgcgcca tgaccagtgc
    23521 ggcaacgcta ccggcaacgc ccgcaccgac cacaatggca tcaaatttgt catccgacat
    23581 gcgttatgct cccttcccgc tgcgtttaca gcgggagatg tggttgaaag ggaatcagcg
    23641 actcaactgg ctaatcaggg cagggacgac tttgtagata tcgcccacca gaccgtagtc
    23701 ggcatagttg aagattggcg catttttatc tttattaatg gcgacaatca cttttgcgcc
    23761 gttgccgcca accatatgct ggatctgccc ggagatcccc agcgtcaggt agagatcgga
    23821 tttcagcaac acgccggaga caccgatata acgttcacgc tccatccagt tctcgccttc
    23881 ggcaattgga cgtgaacagc cgacttcagc attcagcacc gccgccagtt cgtggaccat
    23941 tttcaggtca tcctgcgctg ccagaccacg accgacgcca accacacgtt ttgctttgct
    24001 caggtccacg ctgcttgccg ctttcgcacg gcgttcgcga cagagaattt catgacgcgg
    24061 ggcaacataa ggtaccgttt ctgtcggaca ctgattagag gtatcactgg tgcacggttc
    24121 ctgaacaccg ggcgcaaggg taatgatcgc cagcgggcta ttgatctttt cctgtgcgaa
    24181 cgctaacccg ccatacatcc ggtgttcggc gcaaatgtga ccatcgacaa tatccaccgc
    24241 agtggcatcg ttcaccagcg ccgcattcag ttgcacactt aaccgtgcag ccagagcttt
    24301 accgcgtttc gtcgcggcca gcagcaacat agaagggtgt ttatctttca gcagggcagc
    24361 aatgctttcg gcgtaatttt cagtacgttg cagcgcgtcg ttttgcgcaa gaacataaat
    24421 gcattttggc ccataaggca taactgtctg cgcctggtcg gtattttgta caatggcata
    24481 cacctgttgg ccccattgct gagcgccgcc aaacagttca gcataacgtt caggattatc
    24541 gctaaagacc cagacgctgt ttaattgact catcgtttct tctccgtagg cttcagttca
    24601 gggctttctt cagatgttcc gccagctcgg caatggcctc cggcgaatcg ttatcgagaa
    24661 tgatgtgctt acgttctgtt tgcggcggta cgcgaatgcc ggtcagttca gcaagtggtg
    24721 cgctctggct ccagccaata tcacttgcct gccactgatt taccggtttt ttacccgcgc
    24781 cgaggatggc tttcatcgaa ggaatacgtg gtacgttaat atcggaggtg acgcagagca
    24841 cggctggaac agagagttca ataacttcaa catcatcttc aagcgtgcgt tcaatcacca
    24901 gtgtattgcc ctgacgctga atggcactca ctgcattaat caccggaagt tgcagaattt
    24961 ctccgaccag caagccaacc tgctgggcat aaaggtcgcc ggaaccttca ccaaagatca
    25021 gtaaatcgaa gccgatcttt tcaactgctg tcgccagcgc ctttgcggta tcgagaggca
    25081 gtgcatgctc aagttgtgca tcctgcacca tatacaggct gtgcggcccg cgggatagca
    25141 cgtctttgcg cactttcgag ttctgcaaca aagagccgcc gacggtcagc gcggctatct
    25201 catcgtcatc tgttgcaagc tggcttgcag cttcaatggc attgagatcg aactggctga
    25261 ttttggcgtc ggcattgtcg aaattcaggg tatattctgg agtgacaaca atgtcctgtt
    25321 cttcaggcac cagcttaaag caggttatta ttttcatgat ttctcctgtg agtcgcatac
    25381 ggaaccatct ccgatggaag gtaaaaatgg cctgaaaata gtgtgcgata gcggtgcggg
    25441 gttttcgacg gagcaagctg ttttccttgc caacaatctt gcaaaaaaaa tgcgatcgct
    25501 aaaacaaaag tgcaatttgc ggcaatgttg ttattccatg accgtacaat cttgcattca
    25561 gtgtaaataa tcttgtttag ctctcgctaa acgcggttgt ataaccgcaa tacacgccgt
    25621 attttatccg gcatatattg tttttgcttg ctcatggaat agggaggttg tcatgtatca
    25681 acgctgtttt gataacgcca gcgaaacgct gtttgtcgcc ggtaaaacgc cacggctttc
    25741 acgttttgca tttagcgatg atccaaaatg ggagtccgga catcacgttc atgacaatga
    25801 aaccgagttg atttacgtca agaaaggggt agcaaggttt accatcgatt cttcgttata
    25861 tgtcgcgcat gccgatgaca ttgtggtgat agaacgtggc aggttgcatg cggtggcctc
    25921 tgacgttaac gatccagcaa cgacgtgtac ctgtgcgctg tacggctttc agtttcaggg
    25981 ggctgaggaa aatcagctac tgcaaccgca ttcttgcccg gtaattgccg cagggcaggg
    26041 aaaagaagtc attaaaacct tatttaatga gctaagtgtg attttgccgc aaagtaaaaa
    26101 tagccaaaca tcttcgttat gggacgcatt tgcctatacg ttagcaattc tttactatga
    26161 aaactttaaa aatgcttatc gttcggagca ggggtatatt aaaaaagatg ttctgataaa
    26221 agatattctt ttctatctga ataataatta tcgcgaaaaa atcactttag agcagttatc
    26281 gaaaaagttt cgtgccagcg tcagttatat ttgccatgaa tttaccaaag agtatcgcat
    26341 ttcccctatt aactatgtta ttcaacggcg tatgacggaa gcgaaatggt cactcactaa
    26401 tattgaatta tcacaggcag agatctcctg gcgtgtgggt tatgaaaatg tcgatcactt
    26461 tgccaaactg tttttgcgcc atgtcggctg ttcgcccagc gattaccgca ggcaatttaa
    26521 aaactgtttt gcggaacaag aaatcctatc tgaatttcct caaccagtaa gtcttgccgg
    26581 ataaatctgg cggcgcagtt tgttaagaag ctgcgccgcc tgcagattat ttgttctgat
    26641 aatctttcag gatctgccga cctgctacgt aaatcataat ttcgtctgta ccgccgccga
    26701 tacgttcaca acggacatca cgccagaagc gggagacgcg cgcctcatcg gtatagccca
    26761 gaccgcccat gatttgaatc gcatcatcaa tgacttccat tgcggtacgt gcgcagtaca
    26821 gttttgccag cgccgcgctg gtgcgcagtg actgatgctg atcggcttgc catgccactt
    26881 tcaacaccat gttgcgcatg ttgtcgatct taatcgccat cagcgccagt ttttcctgga
    26941 tcatctggtt atgaccaatg ggcttaccaa aagcgatacg ctggttggca tagcgggcgg
    27001 catcttcgaa tgcgcattcg gcaaagccgg tgctgcgcgc ggcgttgatc aggcgctcca
    27061 tctcaaagtt gtacatcaca ttgaggaaac ccattccttc ttcgcccacc atgtcgctct
    27121 cttcaacttc cacgttgtcg agatagactt cgcaggtgct gagcatatgc cagccgattt
    27181 tatgcagcgg gttaatctta atgccgggct tactggagtc gacccaccac agggtgaagg
    27241 cctttttcgg atctttcggt tctggatcgc gcgccagcac cagcatatac gggtactctt
    27301 tcgcaccggt aataaaggtt ttctggccgt tgatgtaaac cttgccgttt ttacgcgtgt
    27361 aagtggtggt ggcgctgttg ttatctgagc ctgcgcctgg ttccgtcaat gccagggcat
    27421 aggcgggatc acccgtttcc agggtgcttt ccgccgtttt acgtagctgc tctgcagaac
    27481 cgaaacggcg catactgtga atacattgac cgttggtgat caaaaatgcc ggggcaccgc
    27541 attttgacac ttccatcagc gccagcattt gggtgacgta atccgcaggg ataccaccaa
    27601 attcttccgg cacgccaagc atggaaatac cgttatccgc cagtgcccgc ataaattcac
    27661 gcgggtatgt cccgttttga tcgcaggtgc ggaaatactc ttccggaaag ttagtcgtaa
    27721 tcagttcgcg aatactggcc agcagcagtt cttgttcttc agttaaagaa aaatccatca
    27781 ttatactcct gttaatgagc cgcttcaggc aggacaaaac ccatcgccgc atcgataaat
    27841 aatctttcgt ccatcagttt gagttctggc gaaatcactg gggtgaaatc cattttgtcg
    27901 agaatatctt tttgtaaatc gacgccagga gcgatttcga ttaaatgcag gccgtcttct
    27961 ttcagcgtga atacggcgcg ctcagtgata taacgaacat ccagccctcg ctcgagcgcg
    28021 atttttccgc taaaagtaat ttctggtagt tcccgaataa atttcttcac ccgtccttcc
    28081 tggacgatat ttaatttgcc gtcggtaatt tctgttttta aactgcccgc agttaatgtg
    28141 ccgcagaaaa cgattttctt cgaagtggca ctgatatcaa taaatccacc ggtgcccatg
    28201 attttaccat tgaatttatg cacgccgacg ttaccgtgct ggtcgacttc agcaaaactc
    28261 aaataacaaa catccagacc accaccgtga taaaaatcaa actgggacgt catatccaga
    28321 atggcgcggg tattcacgtt cgcgccaaag gcgatcccct gtgaagtaat accgccaatc
    28381 ggacctgttt ctaccgtcag aataaagtca tcagcacaac cttcttcccg ggcgaccagg
    28441 ccaatgccgt cagcaatacc gacgccgaca ttccccactg cgcctttgcg catttcgaat
    28501 aatgcacgcc gtgcaactaa tttacgttgg tttaggggca ggctaagttt ggtgctgtca
    28561 tcaagggtga agtcaccaga aataaagcgg ttaaccggcg ctccgccata cagttgggtt
    28621 tgatccggat cgaccaccac aatatccacc agataacccg gaatacggac agatttagga
    28681 tgcagcgtgg ctttcttaac cattttctgc acctgcatca tcacaatacc gccgttattg
    28741 tgcaccgcct gagcaataac caatgcgtcg agatacatca cctcatcttc aaaagtggcg
    28801 tagccttcgc tgtcgcaggt ggtagcgcga atgaaggcga tatctggcgc tatcgctttg
    28861 taatagagat attcttggtt atcaaactcg accagtttaa tcaggtcttc tttagtgact
    28921 tcattcagtt tgccgccttg ctggcgtgga tcgacaaatg ttccgatgcc aatatcacta
    28981 ataataccag gctggtgggc tgcggcggcg cgtaaggttt gtgtaagtac accttgtgga
    29041 tagttataag caataatttt attttgttct gcgagatcag aaatacgcgg cgattgtccc
    29101 cagtgaccgc ataatgccca tttcaccagg ccttcttgcg ccagcggact aataccacgg
    29161 tcggcgcgat cgccaagccc tgttggacta ataatcgata aattacgtgg tgtttgagtc
    29221 tgtttatatt tatcagcaag agcagtaatt aacgtggtgg cttccagaat accgccgcca
    29281 gcgcctaaca cacaaagtgt tgcttcgtcg ggaatataat tcaccgcttc ctgtgccgac
    29341 aggaccggca ctcgtccatt aatgcgaggt ggttttacag gtttcattgt taattcctga
    29401 ttcaggtgtg attttcccta cgccagatgg agaattagcg cacagagact cacaaaatat
    29461 ttttattaaa tacggttaaa tatatttttt tagttcggcg ggggaggata ttcccgccga
    29521 aatattattg cttatgcctg atgtaaaata gttaatactg tgcgcaaatc atttaccgag
    29581 atttgccctg gtgcagaggc tttttttacc gcaccaaaag ttgccgccga gccaaatact
    29641 tcaccagcca gacgagaaat tacgccagtt tttgccatcg acatcgtgat gattggacga
    29701 tcggcatact gctcctgcat ctccagggtc gcggcaagca acgtcagcac atcgctggta
    29761 ctttgcggca tcagcgcaat cttaggaata tcggcgtcga aggattgcat tttgcgcaga
    29821 cgggcaatga tttcttcggc ttccggcgtt ttatggaagt catggttgga catgacaact
    29881 ttcacatcat gcgcgtgggc gtaggcgacg gtttctttaa cctgatcatc gccggtaaat
    29941 aactccagat cgatcatatc aaccaggccg ctgtcgatgg ctgcacgatt gagtgcaata
    30001 taagcctcgg tggaaatcgc ctgctcgccg ccttctttgg cactgcggaa ggtaaacagc
    30061 agcggttttt ctggcatggt ttcacggaga atttttgctg ccgctatgac agactccaca
    30121 ttggagagat cggcatagtg gtccacacgc cattccagaa tatcaaagtc cgcttcacga
    30181 taggcgagag cttcggattt cacgctggcg atatctttcg ccatcagcga gacgatgatt
    30241 ttaggtgcgc ccgtaccaat gacgagatct tttacagtta cggttttcat tttttaccct
    30301 ttctgcacgc ggtcagcctg tcaggcaccg aaccccatga cctgtttaac atattccaga
    30361 gggaaatctt tgccagtcca taacgtgaac tgttcagccc cttgccacaa caacatgccg
    30421 tatccatcaa tcgttttgca accagcttgt tgcgcctgct gcaataactt cgtcatatgc
    30481 gggttataca cgcattcagt gaccagaagt cccggatgta acagactgat atcattaacc
    30541 aatgattcat tctcaagggg tttcatgccc acttttgtgc cattggttaa aatgtcggcg
    30601 gacgccaggg cttcagcaaa ggcttgctga tcggcgagat cggtaaccgt gacgacacaa
    30661 tcggtgtttt cgttaacccg ctgcgcgaag gcgagggctt tatcgaagaa ctcatcccga
    30721 cggttaaaga gtttaatttc ttttaaacct tcaattgccc cctgcgcgcc aattgccgtt
    30781 gaggcaccac cggcccctaa tagcaccatc gttttgcctt tgatatcaaa accgctctct
    30841 ttaatggcgc gaatatgacc cgtgccgtcg gtgttatagc cacgcagata gccatcatca
    30901 ttaacgatgg tgttgatggc acccaccagt ttggccgctg gcgttaattc atcaacatat
    30961 tcacacgcca gttgtttgtt cggcatcgac acaccagttc cgcgcatttt gagggctttt
    31021 aatccttcaa ttgctccggg aaaactatcg ttatccactt caaaggccat ataggtaaat
    31081 ggcaatcccg ctttttctaa ggctttattc tgcatttcgg gcgataaact gtggcggatg
    31141 ggataggcca tcaacccaat caattcgtat tttgcggtaa catccataat taactccttt
    31201 aacctctatg cttaattgtg tttaaccggg tgctaaattt atgctcgcca aaccgcacat
    31261 cattttcagg aataatgaaa acgcgataat agcggataaa aacaataatt gcggtaatca
    31321 gggccagcag cgcgaaagta aaatcgagag cgatgatata ttgcaggccg atgttcgaca
    31381 gataaccggt aatcagcgga ataacaaagt tagccagtcc acccatcatc atataaatac
    31441 tggtgacttt ggctttgctt ttggggaaaa actctgacat gaccgaaacg ccgagctgta
    31501 aaatgccgcc agctgctgag aaaccgataa caaaggctcc ggcattacac accagtggag
    31561 aagggtacag gtagataatg gctgctgtta ttgttgccag tgcagagtta aatacattag
    31621 cccagatggg ccggaccatt tttttcagta gtgcggcaaa aataaagaca cagaccaacg
    31681 agcccatact gtaataagag atggttttta atgcttcagc ttctgacata ccagcaaaag
    31741 ccatcgcata tttgggcatc cacaccacaa tcacataaaa ggtcgcgaat gcggctacac
    31801 cgaacagtac cgatgaaaca ccttccagcc agactaacgg tttgctgttc atttgcggta
    31861 attcattagc tacgctggcg tccaccaact ggctggggaa tttgcttttc aacagcatca
    31921 gcgtgatcag tacaaacaga atacccggaa taatcagccc atagccgtac cagatgttat
    31981 tgagcaacat atagctcacc agcattgggt agaacatttg cccgaatgac accatcgctt
    32041 taaccagtat gaccgccgaa ccagtggctt tcggaaagca ttccatgagc gcggggtagc
    32101 cacccgtatc cagcgctgaa ttagcgatac ctacgcacac tgccagacag taggcgagag
    32161 ttaaattcgg gcaagcagga ataccaaaga agaatagcag atacattatt actgccatta
    32221 atatcaccgc ccgacgacca aacttatcgg agatcacacc gaagaataaa atactgatca
    32281 atcgccccaa accgataccg gaaattaaat aggcaatgcc cgcgttgtca gtggaaaact
    32341 tttctgccaa agatgtcatg ttttgggcaa gcgtaataac actaatgccg tgcaggaaat
    32401 agctgaagta aatacaaaga acagccagga taaatggcgt gctgaaagcc ttattttgag
    32461 acattttcac agctccttgc tgaaagataa tttcgttacc gtacgtattg ttctccgacg
    32521 gtttacataa ttactatact gaagatgatt ttccctgggt acatggcata aagtttatgt
    32581 ttccaggaat attgcatgtc atagtttgtt tttgatcgcg attttcctta gttagcgcag
    32641 aaagagttat ttatccgtaa gatcttgcat gacctgaaaa gaatattgca ttacccaccc
    32701 ggagcgacat tttcctttag cgagtgatgc cgcgtttctt tgcggctgcg taagccgata
    32761 aacagcgcca gtaaaaagcc gatggcagcg atggcggtat cgaaccacat aatatcggca
    32821 atacttcttt gggacagatg agccgtaatc aacggaatag taaaggtcgc aatactgccc
    32881 gcactgtaat agatccctgt agctttacct ttagcgtagg ggaaacgttc agccattaat
    32941 gtcaggccaa tttgcacaac acctccagca gaggtaaaac caatgacaaa agcaaatatt
    33001 atcaccacat aaaatgtggg atgcaggcag acggtaaaca gagcaataaa tgagataaag
    33061 gtgtacagca tcagtaatgt tgttgggcga acggtattac gaatgagtgg agcggtaata
    33121 aatacacaaa gcagcgaacc cacggtgtag atactgagta gtttgatcga catagtgtat
    33181 gacatgccag caacaaattg tccgtattgt gccagccact ggctaaccag ataaaacgtt
    33241 gccattgaga tatagccata tagggaataa ctggctaaat cgataattga acagcgatgt
    33301 tccgtagagc tggtggtttt ctttatgaca ggtaagcgac gacccggatg gggtgggaac
    33361 gtacaacggc ataaaaacag agcgttaata aacataatgc ctgcggcaat cataaaggac
    33421 caaccgaacc acagttcagc ccacactaac aggctaatta ttagcggtaa taaaaattgt
    33481 ccgctggaaa caaatgcttt aattaaaata ttggctgtcc caggtgagcg tggaaaagct
    33541 tccatcaaac tgggataggt gcctgcatcg agaaaactgt ttgccattcc cgccagaaag
    33601 ccaaaaacat aagcgataat gatgttattg gtgtgcagga tgccaaaaaa gaaggccata
    33661 tagcagcaca tcccgagcat gataaaaggg cggcgaccaa agcgatcgga taataatcct
    33721 gcaaaaagca agacacttaa tcgaccaatg cccagtgatg agataactat cgagacgccc
    33781 gcggcattag tctgccaaag cgtctccagc gaggccatat tcaggctcat caaaatgacg
    33841 cccataccat gcaccaggta attaaaatac aaccccagtg cggtagggta atagggattt
    33901 ttcataggtt ctacttagca aaggtgcttt aaaaagtgta gcgatgttgt gtcagtatcc
    33961 gttcattatt gttgtaacaa agacaattcc tttctgtgga gaaattaaca aagccgctcc
    34021 agatcgtgtg catataattc agcggctacc gattgataga tcttccagtc aataaaattg
    34081 ccatcagggt agacttgttg aaacgcggtt aagtcgggga aaaaacgcat ggtgttgttg
    34141 ccgatgcggt tatttatttt ctctatgatg gcatgaagat gtttttttct ttgctgacgc
    34201 atagccaaca gttgctcgac tacacgatca ggaatggcgt atttgccatt ttcccattga
    34261 cgccagcttt cactgtcacc ggtctgggca atccaggtcg cgcattcatc aatagtcatg
    34321 gcaaaaatat ggcggagtgc ctggagttca taagcgttca tcgtgtaatg gctgcatgaa
    34381 ataaacaata aaatataaat aacattatca gcctgctgac ggcttgaaga gagtcacagt
    34441 atcttgtgca acattattgg aaagcgtgag gggcgaggta gcgaagcgga aaaatgttaa
    34501 aaaaaagccc atcgtgggag atgggcaaag actacacaca gcaattcgtt gtttcactca
    34561 ggggatttcc atgcttataa atcaatatgt tgatttataa ccgtgagcta atagtaggca
    34621 tacggacttt ttgcgtcgat cagattcgtc tcattagttt aaaaactgta aagaatttgc
    34681 gacaggtact accagctgaa tggcggtaag caggaaaata tggggaagag caagcaaata
    34741 tcaattagca ggggttaaca atcaggcggc agagccgcct gatgagaaat tacttattcg
    34801 gtttctcgat ttcgccaagt tcttcgagaa tttcttccgg ttggtccgtc ggcggcggga
    34861 cttcttccac ccacgcggca aacagacgcc aggaaacggc taacagaacc ggaccaataa
    34921 acagaccgat catcccgaaa gcaatcaaac caccaataac gccagagagg atcaggatca
    34981 gcggtaaatc ggcacccatg cgaattaaca ttgggcggat gacgttatcc agcgtgccaa
    35041 ccacaccgct ccacactaac aataccgttc cccaggtggt atcgccggtc cagtagagcc
    35101 agataatcgc cggaatcagt accggcaatg ggccaagctg gacaaggcag gagaggatca
    35161 ttaacaccgt gagcaacgtt gcataaggta cgccggatac ggcgaggccg ataccgccaa
    35221 gcacagcctg tactaacgcc gtcaccacca cacccagcgc caccgcgcgg atagcctgtg
    35281 ccgccagcag tacggcggca tctccgcgaa cgcctgccag acgggttgca aaatggcgaa
    35341 tgccttgtgc cacctgttca ccgcgccagt acagcagggc gctgaagaga agcatcagcg
    35401 cacaatgcac cataaagcgc ccgatatgcg ccgcctgccc aacgaaccag gtggtggtgg
    35461 tgccaatata aggacggact ttcgccatga tcgccgtccc ccccatatcc agcaaattgt
    35521 gccagcctgc atatagcttc gcgccaatca ccggaatggt attaagccac gccagatcgg
    35581 gtaacgtcat gtcaccactg gaaatggctt taattagcgg gccgctgccg tcgacgatac
    35641 tgttaaccag caaagcaata gggatgataa acaccatcac taataacagc gtcatcacca
    35701 gaacggcgag agagcggcga ccaaacatga tcttttgcaa acgtaacaat accggccagg
    35761 tggcgataac cactgtaccg gcccatgcaa agccgagaat aaagggttgt acaatccaca
    35821 gacatgccac aatcatgatg gctaaaaaca gcaccgaaag cagaatttgt gcgacatccc
    35881 tgggctgacg aacatttacc ataactactt ttcacctttt ttgtgcgtca aatcgtcgac
    35941 gcgacgtgaa cacgcgaaac tcacctgcat aatgatgagc aatttcagcg gtttttaaca
    36001 ggccgattct gcccttaatt ctgatgggcg tgttagaaaa aaatgtggta caacaactca
    36061 ggcaacacgc aaacgattta ctcgtcgtat ttcaagttgc atatgctgcg gctacgttcg
    36121 ttcaccccag ttacttactg ttgtaagctc ctggagttca ctcgcttgcc gccttcctgc
    36181 aacacgaaat ccatagagta aacaaacatc acctttcagt gcgctattgc agccgcctga
    36241 aagaacagtg tatcgaccta catcacagac cgcaggaaag ggtcaatata atgattccac
    36301 agatttccca ggcacccggc gtcgttcaac tggtgcttaa ttttttgcaa gagctggagc
    36361 aacaaggttt taccggcgat acggcgacaa gttatgccga tcgtctgaca atgtcgaccg
    36421 acaacagtat ttaccaactt ctccccgatg cggtggtatt tccgcgttca accgcagatg
    36481 tggcgcttat agcccgtctt gccgcgcagg aacgctattc atcgctgatc tttacccccc
    36541 gcggcggcgg caccggcact aacggtcagg cgctcaacca ggggattatt gttgatatgt
    36601 cccgccatat gaaccgcatc atcgaaatta accctgaaga gggctgggtg cgcgttgagg
    36661 ccggggtgat aaaagatcaa ctcaatcagt acctgaaacc gttcggctac ttttttgcgc
    36721 cggaactttc gaccagcaac cgggcaacgc tcggtgggat gatcaatacc gatgcatccg
    36781 gtcagggatc gctggtctat ggcaaaacgt cagatcacgt acttggcgta cgcgcggtgt
    36841 tgttgggggg cgatattctc gatacgcaac ctttacccgt cgaactggcg gaaacgctgg
    36901 gtaaatccaa taccacaatc gggcgaattt ataacacggt ttatcaacgt tgccgtcagc
    36961 aacgccagtt aatcatcgac aacttcccca agcttaaccg ctttcttacc ggttacgatc
    37021 tgcgtcatgt ctttaacgat gagatgaccg agttcgacct gacgcgcatt ctgacgggtt
    37081 cagaagggac gctggccttt attaccgaag cgcgactgga tattacgcgc ttgcctaaag
    37141 tgcgccgtct ggtgaacgtc aaatatgact cttttgactc cgcactgcgt aacgcgccgt
    37201 ttatggttga ggcgcgggcg ctttcggtag agacggtgga ctcaaaagtg ctgaatctgg
    37261 cgcgggaaga tattgtctgg cattccgtca gcgagttgat taccgatgtg ccagacaaag
    37321 agatgctcgg gctgaacatt gtggaatttg ctggtgatga tgagacgctg attgatgagc
    37381 gagtaaatgc actctgtgcg cggctggatg aacttatcgc cagtcagcaa gcaggtgtga
    37441 tcggctggca ggtatgcagt gagctggcag gtgttgagcg tatttatgcg atgcgcaaaa
    37501 aagctgtcgg cctgcttggc aatgccaaag gtgccgctaa gccaattccg tttgctgagg
    37561 atacctgcgt accgccggaa catctggccg attatattgc tgaatttcgc gcgctgctcg
    37621 acagccacgg cttaagctac ggtatgtttg gtcacgtcga tgcaggtgtc ttgcacgtcc
    37681 gtccggcact ggatatgtgc gatccgcaac aagagatttt gatgaagcaa atctctgatg
    37741 atgtagtggc gcttaccgcg aaatacggtg gtttgttgtg gggcgaacac ggcaaaggtt
    37801 ttcgtgccga atacagcccg gcgtttttcg gtgaggaact ttttgcagaa ctgcgtaaag
    37861 tgaaagcggc atttgacccg cataaccgac tcaacccagg gaagatttgc ccgccagaag
    37921 gtctcgatgc accgatgatg aaagtggacg cggtgaagcg cggtacattc gaccggcaga
    37981 tccccattgc ggtacgccag cagtggcgcg gtgcgatgga gtgtaacggt aacggtttat
    38041 gcttcaactt tgatgcccgc agtccgatgt gcccgtcgat gaagattacc cagaaccgga
    38101 ttcattcacc gaaaggacgc gcaacgctgg tgcgtgagtg gctgcgtttg ttggcagatc
    38161 gcggcgttga cccactcaaa ctggaacaag aactgcctga atccggcgtc agtttgcgga
    38221 cgttaattgc ccgcacgcgc aatagctggc atgcgaataa aggcgaatat gacttctcac
    38281 acgaagtcaa agaggcgatg tcgggctgtc tggcctgtaa agcgtgttcg acccagtgcc
    38341 ccatcaaaat tgatgtgccg gagtttcgct ctcgttttct gcagctctat cacacccgtt
    38401 atttacgccc gcttcgcgac cacctcgttg ctacggtcga gagctacgca ccgctgatgg
    38461 cacgcgcgcc gaaaaccttt aacttcttca ttaaccagcc gctggtgcgc aaactctcgg
    38521 aaaaacatat cggcatggtt gatttgccac tgctgtcagt cccctcgcta caacaacaaa
    38581 tggtggggca tcgctcggcg aatatgacgc tggaacagct tgaagctctc aatgcagagc
    38641 agaaagcgcg cacggtgttg gtggtgcaag acccctttac cagctattac gatgcgcaag
    38701 tggtggcgga ttttgtccgt ctggtcgaaa aattaggttt ccagcctgtg ctactgccat
    38761 tttcgccaaa tggcaaagcc cagcatatca aaggctttct taatcgtttt gcgaagacgg
    38821 cgaaaaagac ggcggatttc ctcaaccgtg tggcgaagct ggggatgcca atggtgggcg
    38881 tcgatccggc gctggtactt tgttatcgcg atgaatataa actggctctg ggcgaggagc
    38941 gtggcgagtt taacgtctta ctggcgaatg aatggctggc aagcgcactt gactcacagc
    39001 cagtggctac agtcagcggt gaatcatggt atttctttgg tcactgtacc gaagttaccg
    39061 ctttgccggg cgcgccagca caatgggctg cgatatttgc ccgttttggc gcgaaactgg
    39121 aaaatgtcag cgtggggtgc tgcggcatgg cagggactta cggacatgaa gcgaaaaacc
    39181 ataaaaattc gctcgggatc tatgagttat cctggcatca ggcgatgcag cgactgccgc
    39241 gtaatcgctg tctggcgacc ggatattcct gccgtagcca ggtaaaacgg gtcgaaggca
    39301 cgggggtacg ccatcctgtg caggctttac tggagattat taaatgatat ggaaacgaaa
    39361 aatcaccctg gaagcactga atgccatggg tgaaggaaac atggtggggt tgctggatat
    39421 tcgctttgaa catattggtg atgacaccct tgaagcgaca atgccagtag actcgcggac
    39481 aaagcagcct ttcgggttgc tgcatggagg ggcatccgtg gtactggccg aaagtatcgg
    39541 ttccgttgcc ggttatttat gtaccgaagg tgagcaaaag gtggttggtc tggaaatcaa
    39601 tgctaaccac gtccgctcgg cacgagaagg gcgggtgcgc ggcgtatgca aaccgttgca
    39661 tctcggttcg cgtcaccagg tctggcagat tgaaatcttc gatgagaaag ggcgtttgtg
    39721 ctgttcgtca cgattaacga ccgccatttt gtgacgttgt caccagaaaa gtgtgacgca
    39781 tattgctttt ggtcattatc tgcacagtaa acggtggtat actgtgcagg tcttgtctaa
    39841 aagcgaggat gctatgtcta ctcaacttga tcccacccag ctggccattg aatttttacg
    39901 ccgtgaccag agcaatcttt cacctgccca atatctcaaa cgtctgaaac aactagaact
    39961 tgaatttgct gacctgctta ctctctcttc tgctgaatta aaagaagaaa tctactttgc
    40021 ctggcgtctg ggcgtgcatt gatcctgact taatgccgca attttgccaa atcgacctgg
    40081 attaacctcc gtccaggttc gatgaccttc ttaatactgc ttatctatcc gcctgatatt
    40141 gttcaaaaat aatactaatc gcagtttgtg ttaaaacggc gggttagctt tatgagatta
    40201 ttcttatcgc cccttcaaga gctaagccac tgagagtgcc ggagataagc gccggatggg
    40261 gtagaaaccc ttaagcctgt gtcgcacaga cttaagggtt ttcttatttc tgaacgctgc
    40321 acttttttac ctatctctta aatagtcatt ttcaatacaa ctttttcttt ttcatcacag
    40381 ccttaatgct ccttagataa taatcattat ctaacaatga gatacctaat tcttagcgtg
    40441 cctgttaacc cacacatcag ggtctatgct tattaaaagc aggaaaaaag catgttttac
    40501 ggtaaagccc ctgcgtttgc tgggttgaac tgataatcat tatcactaac atgctgtttt
    40561 acgctgaaag cgatgaagtg aggtaaatcg atggacatgc attcaggaac ctttaatcca
    40621 caggatttcg cctggcaggg cttaacgctg acacccgcag ccgctgtcca catccgcgag
    40681 ctggtggcaa agcagccggg tatggtcggc gtgcgcttag gcgtgaagca aacgggctgc
    40741 gcgggctttg gctatgtgct cgacagtgtt agcgagccgg acaaagacga tctgctgttt
    40801 gaacacgacg gcgcgaagct gtttgtcccg ctgcaagcga tgccgtttat tgatggcacg
    40861 gaagtcgatt tcgttcgtga aggacttaat cagatattca aatttcacaa ccctaaagcc
    40921 cagaatgaat gtggctgtgg cgaaagcttt ggggtatagg cggtactatg tctcgtaata
    40981 ctgaagcaac tgacgatgtc aaaacctgga ccggcggccc gctgaattat aaagaaggat
    41041 tcttcaccca gttagccacc gatgagctgg caaaggggat aaacgaagag gtggtgcgcg
    41101 caatttcggc gaagcgtaat gagccggagt ggatgctgga gtttcgtctc aacgcctatc
    41161 acgcatggct ggagatggaa gaaccgcact ggttgaaagc gcactacgac aagctgaatt
    41221 atcaggatta cagctactac tcggcaccat cgtgcggtaa ttgtgacgac aactgcgcgt
    41281 ctgaacccgg cgcggtgcag caaactggcg cgaacgcctt tttaagtaaa gaggtggagg
    41341 cggcgtttga gcagttgggc gttcccgtgc gggaaggcaa agaggtggca gtggatgcca
    41401 ttttcgactc tgtttcggtt gccactactt atcgcgaaaa actggcggag cagggaatta
    41461 ttttttgttc ctttggcgag gcgatccacg atcacccgga actggtgcgt aaatatctcg
    41521 gcaccgtggt gccggggaat gacaacttct ttgccgcact taatgcggcg gtagcctctg
    41581 acggtacgtt tatttatgtg cctaaaggcg tgcgttgccc gatggaactt tccacctatt
    41641 ttcgcattaa cgcggaaaaa accgggcagt ttgagcgcac cattctggtg gccgacgaag
    41701 acagctatgt cagctacatt gaaggctgtt ccgctccggt gcgtgacagc tatcagttac
    41761 acgcggcggt ggtggaagtc atcatccata aaaacgccga ggtgaaatat tccacggtac
    41821 aaaactggtt ccctggcgat aacaacaccg gcggtattct caacttcgtc accaagcgtg
    41881 ctttgtgcga aggcgaaaac agcaaaatgt catggacgca atcagaaacc gggtcagcga
    41941 ttacgtggaa atatcccagc tgcattttgc gcggcgataa ctccattggt gagttttact
    42001 cagtggcgct gaccagcggt catcagcaag cggataccgg caccaagatg atccacatcg
    42061 gtaaaaacac caaatcgacc attatctcga aagggatctc tgccggacat agtcagaaca
    42121 gttatcgcgg cttagtgaaa atcatgccga cggcaaccaa tgcgcgcaat ttcactcagt
    42181 gcgattcaat gctgattggc gctaattgtg gggcgcatac cttcccgtat gtcgaatgtc
    42241 gcaataacag cgctcagctg gagcacgagg caacgacatc acgtattggt gaagatcaac
    42301 tgttttactg cctgcaacgc gggatcagcg aagaagatgc catctcgatg attgttaacg
    42361 gtttttgcaa agacgtgttc tcggaactgc cgctggaatt tgccgttgaa gcacaaaaac
    42421 tcctcgccat cagtcttgaa cacagcgtcg gataaggaat aaacatgtta agtattaaag
    42481 atttacacgt cagcgtggaa gataaagcta tcctgcgtgg attaagcctc gacgttcgtc
    42541 ccggcgaagt tcacgccatt atggggccca atggttcggg caaaagtacc ttatcggcaa
    42601 cgcttgccgg gcgagaagat tatgaagtga cgagcggcac ggttgagttt aaaggcaaag
    42661 atttgcttgc gctgtcgccg gaagatcgtg caggtgaagg catctttatg gccttccagt
    42721 atccggtgga aattcccggc gtcagtaacc agtttttcct gcaaacggca cttaatgcgg
    42781 tgcgcagcta tcgcggccag gaaacgctcg accgctttga ttttcaggat ttgatggaag
    42841 agaaaatcgc tctcctgaag atgccggaag atttattaac ccgttcggta aacgttggtt
    42901 tttccggcgg cgagaaaaag cgcaacgata ttttgcaaat ggcggtgctg gaaccggagt
    42961 tatgcattct tgatgagtca gactccgggc tggatattga cgcattaaaa gtggttgccg
    43021 atggcgtgaa ctcgctgcgt gacggcaagc gttcattcat cattgttacg cactaccaac
    43081 gcattctcga ctacatcaag cctgattacg tccatgtgct gtatcaggga cgaattgtga
    43141 aatccggcga tttcacgttg gtcaaacaac tggaggagca gggttatggc tggcttaccg
    43201 aacagcagta acgcactgca acagtggcat catttgtttg aagctgaagg ggcgaaacgc
    43261 tccccgcaag cacagcagca tttacaacaa ttgctgcgta ccggactgcc gacacgtaaa
    43321 catgaaaact ggaaatatac gccgctggac gggctgacca atagccagtt tgtcagcatt
    43381 gcgggggaga tatccccaca gcagcgtgat gccttagcgt taacattaga cgccgtgcgg
    43441 ctggtgtttg tcgatggacg ttacgtgccc gcactgagcg atgcaactga aggcagcgga
    43501 tatgaagtga gcattaacga cgaccgtcag gggttacccg atgctattca ggcggaagtg
    43561 tttctgcatt tgacggaaag cctggcacaa agcgtgacgc atatcgccgt gaagcgcggt
    43621 caacggccgg caaagccatt gctgttaatg catatcaccc agggcgtggc aggtgaagag
    43681 gtgaatactg cccattaccg gcatcatctg gatctggcgg aaggtgccga agcaacggtg
    43741 atcgaacatt ttgtcagcct taatgatgct cgtcacttca ccggcgcacg gttcactatc
    43801 aacgtcgcag cgaacgccca tttgcagcat atcaagctgg cgtttgaaaa cccgctcagt
    43861 caccactttg ctcataacga tttgttgctg gctgacgatg ccaccgcatt tagccacagt
    43921 ttcctgctgg gtggcgcagt attacgacac aacaccagta cgcaactcaa tggcgaaaac
    43981 agcacgctgc ggatcaatag cctggcgatg ccggtgaaaa acgaggtgtg tgatactcgt
    44041 acctggttgg aacacaataa aggtttttgt aacagccgac agttgcacaa aactatcgtc
    44101 agcgacaaag gccgcgcggt atttaacggt ttgatcaacg tcgcgcagca cgccatcaaa
    44161 acggatggtc agatgaccaa caataatctg ctgatgggca aactggcgga agtggatacg
    44221 aaaccgcagc tggaaatcta tgcagatgat gtgaaatgca gccacggcgc gacggtgggg
    44281 cgtattgatg acgaacagat gttttatctg cgctcgcgcg ggatcaatca gcaagatgcc
    44341 cagcagatga tcatttacgc ctttgctgct gaactgacgg aagcactgcg tgatgagggg
    44401 cttaaacagc aggtgctggc ccgaatcggt caacgactgc caggaggtgc aagatgactt
    44461 tttccgtcga caaagtgcgg gccgactttc cggtgctttc tcgtgaggta aacggtttgc
    44521 cgctggctta tctcgacagc gccgccagtg cacagaaacc gagccaggtg attgacgccg
    44581 aggccgagtt ttatcgtcat ggctacgcgg cggtgcatcg cggtattcat accttaagcg
    44641 cccaggcgac cgagaaaatg gagaacgtgc gcaagcgggc atcgctgttt attaatgccc
    44701 gttcggcgga agagctggtg ttcgtccgcg gcacgacgga agggatcaat ctggtcgcca
    44761 atagctgggg taacagcaat gtgcgggcgg gcgataacat catcatcagt cagatggagc
    44821 accacgctaa cattgtcccc tggcagatgc tttgcgcacg cgttggcgca gagctgcgtg
    44881 tgatcccgct caaccccgac ggtactctgc aactggagac gctgcctacg ctgtttgatg
    44941 agaaaactcg tctgctggca attactcatg tctccaacgt gcttgggaca gaaaatccgc
    45001 tggcggagat gatcacactt gcgcatcaac atggcgcaaa agtgctggtg gatggcgctc
    45061 aggcggtgat gcatcatccg gtggatgttc aggcgctgga ttgcgatttt tacgtgttct
    45121 ccgggcataa actgtatggc cctaccggaa ttggcattct ttatgtcaaa gaagccttgt
    45181 tgcaggagat gccgccgtgg gaagggggcg gttctatgat cgccaccgtc agcctgagtg
    45241 aaggcactac ctggaccaaa gcaccatggc ggtttgaagc cggtacaccc aataccggag
    45301 gcatcattgg tcttggcgcg gcgctggagt atgtttcggc gctggggctt aataacatag
    45361 ccgagtatga acagaatctg atgcattacg cgttatcaca gttggaatct gtaccggatc
    45421 tcactctcta tggcccacaa aacaggcttg gcgttattgc ttttaatctc ggtaaacacc
    45481 acgcctatga tgttggcagt tttctcgata attacggcat tgctgtgcgt accggacatc
    45541 actgcgcaat gccattaatg gcctattaca acgtccctgc gatgtgtcgg gcatcgctgg
    45601 ccatgtataa cacccatgaa gaagtggatc gtctggtgac cggcctgcaa cgtattcacc
    45661 gtctgctggg ataacaggga ggcactatgg ctttattgcc ggataaagaa aaattgctgc
    45721 gtaatttttt acgctgcgcc aactgggaag agaaatatct ctacattatt gagctgggcc
    45781 agcgtctgcc agaattacgc gacgaagacc gaagcccaca aaatagcatt cagggttgcc
    45841 aaagtcaggt gtggattgtg atgcgtcaga atgcccaggg aattattgaa ttacagggcg
    45901 acagcgatgc ggcgattgtg aaagggctta ttgcggtcgt ctttattctt tacgatcaaa
    45961 tgacgccgca ggatattgtc aatttcgatg tgcgcccgtg gtttgaaaaa atggcgctca
    46021 cccaacatct caccccatca cgctcacaag gtctggaagc gatgattcgc gcaattcgcg
    46081 ccaaagccgc tgcacttagc taaactaaag ggacagcttt catccggttg gctcacattg
    46141 ccgggtggtc aatgtttaca ctggcctgca caggagtgtt ttggtttcag gtgaacataa
    46201 gcgccatccg acgtttcagc gtgagtctcc ggcaaataca ggaggtttac aatgaaacgc
    46261 gcgtctttgc ttacactcac gcttatcggc gcttttagcg ccatccaggc tgcctgggcg
    46321 gttgattatc cgctaccacc aaccggcagt cgactggttg ggcaaaatca aacgtatacg
    46381 gtgcaagaag gggataaaaa ccttcaggcc atcgcccgac gttttgatac tgcggcaatg
    46441 ttgatccttg aagccaataa cactatcgcc ccggtgccaa aacccggtac gacgataact
    46501 attccttcac aactattatt acctgatgcg ccgcgtcagg ggattattgt aaaccttgca
    46561 gagctgcgcc tttattatta tccgccggga gaaaatatcg tgcaggttta tccgataggt
    46621 attggtttgc aggggctgga aacgccggtg atggaaacgc gtgtggggca gaaaatccct
    46681 aacccaacct ggacgcctac ggcgggcatt cgtcaacgtt cgctggagcg tggcattaaa
    46741 ttaccaccag tcgttcctgc cgggcccaat aacccactag gacgttacgc actgcgcctc
    46801 gcgcatggta atggcgaata cctcattcat ggcaccagtg cgccggacag cgtcggtttg
    46861 cgcgtcagtt cgggctgtat tcgcatgaat gcaccggata ttaaagcttt attctccagc
    46921 gtgcggacgg gaacactggt gaaagtgatc aacgaaccgg tgaaatattc cgtggaacct
    46981 aacgggatgc gttatgttga agtacatcga ccactatcgg cagaagaaca gcagaacgtt
    47041 cagacaatgc cgtacacgct gccagcaggc tttacgcaat ttaaagacaa taaggctgta
    47101 gatcagaagt tagtcgataa agcgttgtat cgtcgggcag ggtatccggt tgcggtgagc
    47161 agtggagcaa ctcccgcagc cagcaatgcg ccttcagtag agtcagcgca gaatggggaa
    47221 ccagagcaag ggaatgtgtt acgcgcgacg cagtagcggt aaatagcagg caaaaaaatg
    47281 gcgcacaatg tgcgccattt ttcacttcac aggtactatt acttgcggta tttagtagcc
    47341 atgttgtcca gacgctggtt agcacgagct gcgtcatctt tagcagcctg aacgtcggaa
    47401 cgcattgcgt tcacgtcgtt gctcagctgg tcaactttag cgttcagagt ctgaacgtca
    47461 gaagacagct gatcgatttt agcgttgctg gagcaacctg ccagcagagt agaacccagg
    47521 attaccgcgc ccagtaccag tttagtagct ttcattatta ataccctcta gattgagtta
    47581 atctccatgt agcgttacaa gtat
//
LOCUS       DAPHCV010000023        46487 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.023, whole genome shotgun sequence.
ACCESSION   DAPHCV010000023 DAPHCV010000000
VERSION     DAPHCV010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.023"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            58..798
                     /locus_tag="RH033_001328"
     CDS             58..798
                     /locus_tag="RH033_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HDV2990542.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDTFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMVDRNESFTFVTGVSWRF"
     gene            complement(928..2898)
                     /locus_tag="RH033_001329"
     CDS             complement(928..2898)
                     /locus_tag="RH033_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HDV2990543.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQDGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNAYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLE
                     VVCRLADHIDSVFGPGESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPIAQQQTAIGHTVRFVYLMTG
                     VAHLARLSHDESKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKT
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQADN
                     GESLHNLWLPADAPFTTFEGKGLFRHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     PQTLTFIPWFSWANRGEGEMRIWVNEEKHCHP"
     gene            complement(2909..4309)
                     /locus_tag="RH033_001330"
     CDS             complement(2909..4309)
                     /locus_tag="RH033_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2990544.1"
                     /translation="MTSTPITAADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTTSSLGGITAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIASDLRQRHVQP"
     gene            4535..5350
                     /locus_tag="RH033_001331"
     CDS             4535..5350
                     /locus_tag="RH033_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV2990545.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDNDPRWLTHLS
                     VPQQTKVADRQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADAAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            complement(5382..6077)
                     /gene="araD"
                     /locus_tag="RH033_001332"
     CDS             complement(5382..6077)
                     /gene="araD"
                     /locus_tag="RH033_001332"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDV2990546.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKAVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTVEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(6071..6931)
                     /locus_tag="RH033_001333"
     CDS             complement(6071..6931)
                     /locus_tag="RH033_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDV2990547.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRASLVTAMIETGVGIPSMCLSAHRRFPFGSRDEAVRERAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            complement(6924..7586)
                     /gene="ulaD"
                     /locus_tag="RH033_001334"
     CDS             complement(6924..7586)
                     /gene="ulaD"
                     /locus_tag="RH033_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDV2990548.1"
                     /translation="MSRPLLQLALDHSSLEDAQRDVTLLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPNKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGAANPVQVAGDFHAQIDAIWGGA
                     RA"
     gene            complement(7583..9079)
                     /gene="lyxK"
                     /locus_tag="RH033_001335"
     CDS             complement(7583..9079)
                     /gene="lyxK"
                     /locus_tag="RH033_001335"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HDV2990549.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRALLTHSGVSGEQIVGIGISAQGKGLFLLDKNDKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPLVVGSAEIC
                     GEITAQTAALTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGQFIVHEASPTSSGNLEWFTTQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRVHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTAHQLYQQKYQRYQHLIAALQGFHARIKEHTL"
     gene            complement(9083..10069)
                     /gene="yiaO"
                     /locus_tag="RH033_001336"
     CDS             complement(9083..10069)
                     /gene="yiaO"
                     /locus_tag="RH033_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter
                     substrate-binding protein YiaO"
                     /protein_id="HDV2990550.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKVSLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLTPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGAELLKAVDEVQ"
     gene            complement(10082..11359)
                     /gene="yiaN"
                     /locus_tag="RH033_001337"
     CDS             complement(10082..11359)
                     /gene="yiaN"
                     /locus_tag="RH033_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HDV2990551.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            complement(11362..11835)
                     /gene="yiaM"
                     /locus_tag="RH033_001338"
     CDS             complement(11362..11835)
                     /gene="yiaM"
                     /locus_tag="RH033_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HDV2990552.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            complement(11953..12420)
                     /locus_tag="RH033_001339"
     CDS             complement(11953..12420)
                     /locus_tag="RH033_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDV2990553.1"
                     /translation="MIFGHIAQPDPCRLPAAIEKALDFLRTTDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAAENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTTSEIRKIVVKVALTALN"
     gene            complement(12432..13430)
                     /gene="yiaK"
                     /locus_tag="RH033_001340"
     CDS             complement(12432..13430)
                     /gene="yiaK"
                     /locus_tag="RH033_001340"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HDV2990554.1"
                     /translation="MKVTFEQLKAAFNRVLISRDVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            13631..14479
                     /gene="yiaJ"
                     /locus_tag="RH033_001341"
     CDS             13631..14479
                     /gene="yiaJ"
                     /locus_tag="RH033_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HDV2990555.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            14581..15054
                     /locus_tag="RH033_001342"
     CDS             14581..15054
                     /locus_tag="RH033_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDV2990556.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHTWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(15206..16459)
                     /gene="avtA"
                     /locus_tag="RH033_001343"
     CDS             complement(15206..16459)
                     /gene="avtA"
                     /locus_tag="RH033_001343"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HDV2990557.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(16637..18667)
                     /gene="malS"
                     /locus_tag="RH033_001344"
     CDS             complement(16637..18667)
                     /gene="malS"
                     /locus_tag="RH033_001344"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDV2990558.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQLDTRSGTPT
                     LMISIQNAVEPVASLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDAPAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGSEAALRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKTLDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQDFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ASVAHWQKISQFRARHPAIGEGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            18987..19811
                     /locus_tag="RH033_001345"
     CDS             18987..19811
                     /locus_tag="RH033_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HDV2990559.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            complement(19919..21097)
                     /gene="xylR"
                     /locus_tag="RH033_001346"
     CDS             complement(19919..21097)
                     /gene="xylR"
                     /locus_tag="RH033_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
                     /protein_id="HDV2990560.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVHVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(21175..22356)
                     /gene="xylH"
                     /locus_tag="RH033_001347"
     CDS             complement(21175..22356)
                     /gene="xylH"
                     /locus_tag="RH033_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HDV2990561.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQSLTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            complement(22334..23875)
                     /gene="xylG"
                     /locus_tag="RH033_001348"
     CDS             complement(22334..23875)
                     /gene="xylG"
                     /locus_tag="RH033_001348"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HDV2990562.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGCLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            complement(23953..24945)
                     /gene="xylF"
                     /locus_tag="RH033_001349"
     CDS             complement(23953..24945)
                     /gene="xylF"
                     /locus_tag="RH033_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV2990563.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            25311..26633
                     /gene="xylA"
                     /locus_tag="RH033_001350"
     CDS             25311..26633
                     /gene="xylA"
                     /locus_tag="RH033_001350"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDV2990564.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFHHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            26705..28159
                     /gene="xylB"
                     /locus_tag="RH033_001351"
     CDS             26705..28159
                     /gene="xylB"
                     /locus_tag="RH033_001351"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDV2990565.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDLMLQACHLSRDQMPALYEGSEITGALLPEVAK
                     AWGMAAVPVIAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGVLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALITAARQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            28328..28669
                     /locus_tag="RH033_001352"
     CDS             28328..28669
                     /locus_tag="RH033_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2990566.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDDFFIHVCQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSA"
     gene            28715..29152
                     /locus_tag="RH033_001353"
     CDS             28715..29152
                     /locus_tag="RH033_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HDV2990567.1"
                     /translation="MDNKISTYSPAFSIVSWVALIGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTPIYYMTCLTVFIISVALLLVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(29193..30188)
                     /gene="wecH"
                     /locus_tag="RH033_001354"
                     /pseudo
     CDS             complement(29193..30188)
                     /gene="wecH"
                     /locus_tag="RH033_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
     gene            30363..30671
                     /locus_tag="RH033_001355"
     CDS             30363..30671
                     /locus_tag="RH033_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HDV2990568.1"
                     /translation="MMMNSFFPAMALMVLMALVGCSTPPPVQKAQRVKVDPLRSLNME
                     ALCKDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR
                     "
     gene            30766..31677
                     /gene="glyQ"
                     /locus_tag="RH033_001356"
     CDS             30766..31677
                     /gene="glyQ"
                     /locus_tag="RH033_001356"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDV2990569.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            31687..33756
                     /gene="glyS"
                     /locus_tag="RH033_001357"
     CDS             31687..33756
                     /gene="glyS"
                     /locus_tag="RH033_001357"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDV2990570.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNTNVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAASLAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            34078..34230
                     /gene="hokA"
                     /locus_tag="RH033_001358"
     CDS             34078..34230
                     /gene="hokA"
                     /locus_tag="RH033_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HDV2990571.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMVRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(34418..34630)
                     /gene="cspA"
                     /locus_tag="RH033_001359"
     CDS             complement(34418..34630)
                     /gene="cspA"
                     /locus_tag="RH033_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HDV2990572.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(34911..35201)
                     /locus_tag="RH033_001360"
     CDS             complement(34911..35201)
                     /locus_tag="RH033_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HDV2990573.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            35635..36345
                     /locus_tag="RH033_001361"
     CDS             35635..36345
                     /locus_tag="RH033_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HDV2990574.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(36395..37369)
                     /gene="ghrB"
                     /locus_tag="RH033_001362"
     CDS             complement(36395..37369)
                     /gene="ghrB"
                     /locus_tag="RH033_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HDV2990575.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTIEQNAAILA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETYHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(37473..38132)
                     /locus_tag="RH033_001363"
     CDS             complement(37473..38132)
                     /locus_tag="RH033_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HDV2990576.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            38285..40618
                     /gene="bisC"
                     /locus_tag="RH033_001364"
     CDS             38285..40618
                     /gene="bisC"
                     /locus_tag="RH033_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HDV2990577.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALELIHHQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLTRCTTGYD
                     VFASYLLGESDGIAKTAEWAAEICGVNAEKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERIDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWEANQL
                     IEMPENPDSERFIRFADFRRDPQAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDIVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(40587..41027)
                     /locus_tag="RH033_001365"
     CDS             complement(40587..41027)
                     /locus_tag="RH033_001365"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV2990578.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLLL
                     EVYQKNQPAIDFYHAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(41024..41587)
                     /gene="tag"
                     /locus_tag="RH033_001366"
     CDS             complement(41024..41587)
                     /gene="tag"
                     /locus_tag="RH033_001366"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDV2990579.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRASFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            41745..42443
                     /locus_tag="RH033_001367"
     CDS             41745..42443
                     /locus_tag="RH033_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV2990580.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLADPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            42672..43880
                     /locus_tag="RH033_001368"
     CDS             42672..43880
                     /locus_tag="RH033_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2990581.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            44204..45895
                     /gene="eptB"
                     /locus_tag="RH033_001369"
     CDS             44204..45895
                     /gene="eptB"
                     /locus_tag="RH033_001369"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HDV2990582.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVREGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAKEAAAN"
     gene            45987..46063
                     /locus_tag="RH033_001370"
     tRNA            45987..46063
                     /locus_tag="RH033_001370"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46021..46023,aa:Pro,seq:cgg)
     gene            46160..>46487
                     /locus_tag="RH033_001371"
     CDS             46160..>46487
                     /locus_tag="RH033_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV2990583.1"
                     /translation="MMTEFKRTQRDYPLSFKIAVVEQVEKGEMTYKQAQQRYGIQGRS
                     TVLVWLRKYGRLDWSPGLPDLVKRKLPVTQTTIPLTPEQRIRELEAQLELANQKAEFF
                     ESVINVL"
BASE COUNT        11306 a        11587 c        12012 g        11582 t
ORIGIN      
        1 ttgtaagcat tactatgccg actctttttt atcttatttt aaaatggaat accggacatg
       61 ttaattaacc gcaatattgt ggcgttattt gcgttgcctt ttatggcaag cgcaactgct
      121 tctgaattat ccattggtgc tggtgcggct tataatgaat cgccttatcg cggttataat
      181 gaaaatacaa aggcaattcc gctgattagt tatgaaggtg atacttttta tgttcgtcag
      241 accacgttag gttttattct gtcgcaaagt gaaaaaaatg aacttagcct gaccgcatcc
      301 tggatgccgc tggaatttga ccctgccgat aatgacgatt atgccatgca acagcttgat
      361 aagcgtgata gtacggctat ggcgggggtt gcctggtatc accacgagcg ttggggaacc
      421 gtgaaagcct ctgcggctgc ggacgttctg gataacagca acggctgggt gggggagcta
      481 tcggtattcc acaaaatgca gataggtcgc ctgtcgctga cacctgcgct gggcgttctc
      541 tattatgacg agaatttcag tgactattac tatggcattt cagagagtga gtcccgtcgt
      601 agcggtctgg caagttattc cgcgcaggat gcctgggtgc cttatgtcag cctgacggca
      661 aaatatccga taggagagca cgtcgtattg atggcgagcg ccggatacag cgagctgccg
      721 gaagagatta ccgacagccc gatggttgat cgtaatgaga gttttacctt tgtcaccggc
      781 gtgagctggc gtttttaatt caccggtgga tgtcggtgcg gctggcctac gctctcgcac
      841 cgacttgtag gcctgataag acgtgttaag cgtcgcatca ggcaatgtgc tccctacgtt
      901 ggatgcggcg cgagcgcctt atccaaccta cggatggcaa tgcttttcct cattcaccca
      961 gatccgcatt tcgccttcac cccggttggc ccagctaaac cacgggataa aggtcagggt
     1021 ttgtggctgg cgtttggctg gcgcgctgtc gtaatgccac agcggttgct gctctggatt
     1081 gctctgttca taccgataac ccggtgcctg gattaagatc ttatggcgga acaatccctt
     1141 accttcaaat gtcgtaaatg gcgcatcggc gggcagccac agattatgca gtgactcgcc
     1201 gttgtcggcc tgttccaggc aatacaccag cgggccgcgc tgaatcgcca cttttccggc
     1261 gacgtgacgc accagagggt taccgtaaac gcgacgtacc ggcattggca aagtcagatt
     1321 cagcgtgtcg ccctcctgcc attcgcgggt aatgtgcaaa taccctttac gaatatcctg
     1381 ctcgacctct tccccattca atatgatctg cggctgtgtg caccagtccg gcagacgtaa
     1441 agccagcgta tgacgcaccg gctggggcga ttcaaccgca attgtcacct gttcctgcca
     1501 cggatagttt ccgctaaccc gcagacgcag cgtgccattt tctaccggca cttccatgct
     1561 gtttcctgcg tagatgttga tatacaacgc atcttcacgc ggcgtgtaga gataatgacc
     1621 aatcgaggtt agcacgcggg cgatatttgg cggacaacaa gcgcaaccaa accaacgctg
     1681 gcggatcggc ttaacgtgat cgtagatatg gttgaatttt aacgtttttg gatgcacttc
     1741 cagcggatta acgtagaaga aatgtttgcc atccagcgcc atgccgccga gcacagtgtt
     1801 gtacagtgcg cgctccatca catcggcata ttgactgtcg ccttccattt ccagcattcg
     1861 ccgggcgaac atcatcaggc cgatggaagc acaactttcg gcgtaaaccg tgtcattcgg
     1921 cagatcgtaa tcgctgctga acgcttcgcc gctgctttgt gagccaatgc cgccggtaat
     1981 atataactga cgctgggcca tattgttcca cagccgcagg caatcctgac gcttgctttc
     2041 atcgtgactt aaacgcgcga gatgtgcgac gccagtcatc agatatacaa agcgtactgt
     2101 atgtccgatg gcggtttgtt gctgtgcgat gggcaaatgc gcctggctgt aggctttatc
     2161 tttcaccatc cacgccgggc cgtaggtgtg ccagtgcgag gtctgcccgc gcttttcata
     2221 ttcctggtcg taatagtgcg gttgcgcacc acgctgttcg acaaaatagt tcgtcagcgc
     2281 caggtagcgc ggctcttcgg tcacttcata caggcgcatt agtgccagtt caatttccgg
     2341 gtgaccagga taaccgtgta acttactttc acctggacca aatacgctgt cgatatgatc
     2401 ggccagacgg caaaccactt ccagcaagcg ccgttttccc gtggcctgga agaaggcgac
     2461 tccggcttca atcagatgac cggcgcagta aagttcatga cactccgcca gattgctcca
     2521 gcgttcttcg ggcgctttta ccgtaaagta agcattgaga tagccatctt cgcactgggc
     2581 ggaggcgatc agttcaatca cctcgtcagc ggttttttcc agttcggcat ccggcttctg
     2641 gcacagcgac caggctaccg cttccagcca ttttgcgacg tcgctgtcct gaaacaccat
     2701 cccgtaaaat tcaccgtctt gcagtcctgc ggcaatgcga aagttttcaa tcgcatggct
     2761 gggttccgct tctgggatac gatcgttcaa ggcgtcccac tgataaggaa tcaccacatc
     2821 gcggaccagt tgttggtact gaccgaggaa cggatcgctg accgtcagtt tatgcagatc
     2881 gacttccgaa atgttcatct tgtgctcctt aaggctgtac atgacgctga cgcaggtcgc
     2941 tagcaatgcg tgacatcata gggttattga gtttgcaaaa gcggatcgcc cctgccagca
     3001 gcaggtggaa tagcgcaggc agtaatgttt ccatcgccgt gatcccgcca agcgaactgg
     3061 tggtctggtt ttccacgcct ggctggtagg cgacaaaaat aaacagcagg ctgatgatcc
     3121 cggcgctgga ggcccatgcc agcttgatga aaaacagatt gaaggcgaag ttcatgccgg
     3181 aagaacgtac gcgggttttc cactcgccgt agtcatcggc aaaggccatc aatgagaagt
     3241 gcagtggcaa tgtgaagcca agaattacgc cattaccgag gatcaccgcc agccacagcg
     3301 tctgataagc cgggccagaa ggcaggaacc acatcaacac cgccagcgca gcgaggagca
     3361 ggttggtgta gtagtaaatt ttgacggtat cgaaacgccg cgttaacggg ctgacaatca
     3421 ccgagccgat aatggaggcg aaggtgacca tggtgaagaa cagcgacgtg tagcccgtac
     3481 tgccttgtaa gacgtaggta atgaagtaca tatacccacc gccgcgaata ttaaagacgt
     3541 taatcagcag gaaagacatg accagcatca gcagcagttg gtcgttgttc cgcagcccgg
     3601 caagatgttc gcgcagggta aatttcccca ttgtggagag cggcacccgt tcacgaaccc
     3661 agaagaaaca gcacaggaac atcaccacgg caatggcgca cagcacgccg acgcccagtt
     3721 gatagccgcg cgcggcatta ccctgaccga agagatccac catccacggc aggccaaccg
     3781 aaaccagaaa tcccgctacg ccgcagagaa cgaatcgcca ggactggcag gagatcactt
     3841 cattgtggcg ggtggtcatg gtgttgatca gcgcgcagta cggtacgttg atggcggtat
     3901 aaccgactga aaggagcagg taagtaccga acgcccaggc gatttttact cccatgctgg
     3961 catctggcac ggtgaacgtc aggatgccga taatgccgat gggaatggca atccataact
     4021 gccacggacg aaagcgcccc cagcgacttt gcgtccggtc ggcaatgacg cccatcaccg
     4081 ggtcggagat ggcatcaaaa acgcgcagtg caataaacag cgtgccgacc agcgccggag
     4141 ttaagccaaa aacgtcggtg tagaaaaagg tgagaaagtt catgatcagg caagttatta
     4201 cggtgccgcc tgcgtcaccc aggccgtagc ctattttttc ccgtacagat aacgcatcgt
     4261 caattttacc tttagcgata tcagcggcag taatcggtgt agaggtcata gatattcctc
     4321 gtgaataggg agcaattttt agttttattg caatgacctg attaattctg ccgtgaacgg
     4381 gaatatcaac aaggcgagaa aagagtaaaa aaaggtgacg attccgacct gaatttaaaa
     4441 gtgtgaaatg gatcaggtga aattaatgtt tttgattaat aatcaaaagg taatgtgttt
     4501 aatcattaat gaataagcgg gaaattacat atcaatgctc gaattatcca tgacgttacc
     4561 gattaaagtg caaaatggtg gattatttat ttcccgtggc gtagggaggc atccggcaag
     4621 gcggctggag tcatgggaaa ttatctttgt cgaaaaaggt cgattaacga ttcaggaaga
     4681 agagcggata tttctggtcg atgcgggaga gagtttatta ctctggccga accgacagca
     4741 tgttggggta gaggattttc ccgccgatct caaattttac tggctgcatt ttgaggttaa
     4801 agcgccggac aacgacccgc gctggctcac tcatcttagc gtgccgcaac aaacaaaagt
     4861 tgccgatcgg caggcgctgg tggccctgtt tcgccagttc ttaagcgaac aggaaaagca
     4921 tcaacgcact ccggcgctgg agtttattgt gctgctgatt ttgcagcaac tcacccgtga
     4981 cgcgcgtctt gatgaacagg ccgatgccgc aggtaccacg cttgcctgga aagcgcagaa
     5041 actgatccgc acgcaatatc atcgcccgct ttctacggcg cttttagccc gcgagctgta
     5101 ctgcaatgcc gattatcttg ggcgcgtcta ccggcgggtg tttcatctga cgttaacaga
     5161 agcaattcat cgccagcgag tgcgcgaagc cgaaaagctg ttaattagcg aagcgcgatc
     5221 gctcactgaa gtggcggcgt tgtgcgggtt taatgacgtc ggctatttcc ggcagatttt
     5281 tcgcaagcac accggtttaa cgccagctgc gtggaagcga cgttacagca aggaacatat
     5341 caattcgtag tgccggggca atgaagcccc ggcgtgaggg attactgccc gtaataggca
     5401 ttggccccat gcttacgcag gtagtgctta tccagcagtt cgttttgcat cgcagggagc
     5461 tgcggcgcaa gctggcgcga gaacaggccc atataggcgc actcttccag cactacggca
     5521 ttatgcaccg catcggcggc attttttccc caggcgaacg ggccgtgaga atgtaccagt
     5581 accgccggga tttgtgccgg actcctgccg cgttcttcga aagtttcaat gatcacttcg
     5641 ccggtctgat attcatattc gccgttaatc tcctctacgg tcatctgtcg cgtgcagggg
     5701 atggcaccat agaaataatc ggcgtgcgtg gtgccccagg cggggagatc cagcccggcc
     5761 tgtgaccaga tggtggcgtg gcgcgagtgg gtatgcacaa tgccaccgat ttcggcatag
     5821 cgacggtaaa gcgccagatg cgttggcgta tcggacgagg gctttttgct gccttccacc
     5881 gccttaccgc tggctatctc aaccaccacc atatcgtcgg cggtcatcac gtcgtactcg
     5941 acgccggaag gtttgattac catccattgc cgcgtttcgt ctaccgcgct gacattgccc
     6001 caggtgaacg tcaccagatg gtgagcggga agcgccagat tcgccgccag cacgtcggct
     6061 ttcagttgct ctaacatata aatccagcct cctgcatacg cgcttcaatc cagcgccgcg
     6121 cctgaataat ctccagcacc ggctctttgg ctttttcggt ccacatctca atcaggaaag
     6181 aaccgcgata gttcagttca tgcagcgttt tgaagatgcc aacgaaatcg acgcagcctt
     6241 cgccaaacgg cacgtcgcgg aactgtccgg ggctttgctc ggtaactggc tgggtgtctt
     6301 tcaggtggat cgcggcgata cggtcaatgc ccagtttcag ttcggcggga acatcattgc
     6361 cccaggcgct gaggttgccg acgtccgggt agacggtgaa ccacggcgag gcgagcatct
     6421 cgtcccactt tttccatttg ctgatggagt tcataaacgc agtatccata atctccaccg
     6481 ccagcattac ttgtgatgcc gccgcctgtt cgactgccca cgccagccct tcagcaaaac
     6541 gttgccgggt gccttcgtcg tggtcttcgt aatagacgtc ataacccgcc agttgaatgg
     6601 tgcgaatgcc gagatcgcgc gccaggcgaa tcgctttact catgatttcc cgcgcccgtt
     6661 cgcgcactgc ctcgtcacgg ctaccaaaag gaaagcgacg atgtgcggac aggcacatcg
     6721 acgggatccc aacacccgtt tcgatcatcg cagtgaccag cgaagcacgt tgtgcggcgc
     6781 tccagtcaag acgtgagagc cgttcgtcgg tttcatccac cgacatttcg acaaaatcaa
     6841 agccacagct ttttgccagc accagccgct ccggccagga gagatctttc gccagcgctt
     6901 tttcataaat ccctaacgga tgattacgca cgcgcacctc cccagatggc atcaatttgt
     6961 gcatggaaat cgccagccac ctgcaccgga tttgctgcgc ctgccagtgc tcgcccggca
     7021 ataaacgctt tcacgcgaat atctttaaac agcggcaggt cagcaggggt aatcccgccg
     7081 gtaatggaaa gctcaaggcc gatatcggaa agcgccttca tgcgtgccag atcggcctcg
     7141 ccccactgtt gcccgctggc ctgcgcgtcg cgaccgcgat gataaatggc ctgccgcacg
     7201 ccaatacgat gccagtcgcg ggcgtcgtcc agcgtccagt taccgaacaa ctcgatctga
     7261 atttcaccac cgcagcgttg cgccatcgcg tggccttttt ccaccgtcgc gagcggtgcg
     7321 gcgcagatga tggtcatcca gttagcgcct gcgccaaacg cctgttgcgc gagcgtttca
     7381 ccggcatcgg cgaccttcca gtcagcgacg atgattttgt tcgggcactg ttcgcgcaag
     7441 gctttcactg cgccaagccc ttcgtttaaa cagagaatgg tgcccgcttc gacgatatcg
     7501 acgctgtctt ttaacagcgt cacgtcgcgc tgtgcgtctt caagtgatga gtggtcgagg
     7561 gccagttgca gaagtggtcg gctcataatg tgtgctcctt gatgcgggcg tgaaagccct
     7621 gaagtgcggc aatgagatgc tgataacgtt ggtatttttg ttggtaaagc tgatgggcgg
     7681 tcatatccgg cagcagggtg cgtaccgggt gctgtaaatc acgttgggct tcgctgaaat
     7741 cgcgataaac tccggtgccg acgcgggcgg caagtgccgc accaaagcag ccggtttctt
     7801 ccacctgcgg cagctcgata cgcagaccgc tgacgtccgc cagcatttgc atccagacat
     7861 cggagtgcgc cggaccgcca gtgacgcgca gggtgtgcac atcagtaaaa cgttcgcgca
     7921 tccggttgag atgggtcata tggctgaaca ccacgccttc ataaatggcc tgcaacaggt
     7981 gtacgcgggt gtgaatggcc tgcatcccgt agaaaccgct ggtcatctcg agtccagcgt
     8041 tgctgccgta caggaacggc aggaaaaaga gatcgccccc ggccttcggc aagctggcaa
     8101 cggcctggtt gatctcatca aacgagattt ctccccactg tgtggtaaac cattcgagat
     8161 tgccggaaga ggtggggctt gcttcatgaa cgataaattg accatcgtta acgtagcgac
     8221 catagacata cggatgcgct tcaccgtcac gtaaaccacg ggttatgccg ctggtcaccg
     8281 cccaggtgcc catcaccgca ttgagggtaa attcgtcttc gatcccggcg cagagtgcgg
     8341 tggaaaccac atcaaacagg ccgccgacaa cgggcgtacc cgctttcaga ccggtcagtg
     8401 cggctgtctg agcggtgatc tccccgcaga tttcggcaga tccgacaaca agtggcaggg
     8461 cgtggttgat ttcagcgatt cccagccagt cggtgaggca cgggtcatat tccccaagac
     8521 tcatgttgta gagattggac tcagaaatat tgctctcttc gcaacctttg acgccggtta
     8581 aacaccagcg caggtagtcg tgcgtcatca tcacgcagcc aatttgcgcg tagcgttctg
     8641 gttcgtgctc tttcagccag cgtaacagcg acaccggatg cccggtccac aaggtttgtc
     8701 gggtcagcgg gtagagtttt tccgggatgc catcttcctg ccagcgacga acgatttcca
     8761 tcgcccggcg atccgaggac aaaatggcgt taccgagcgg cttgtcgttt ttatccagca
     8821 aaaacaagcc ctttccctgt gcggagatgc cgataccgac aatttgttcc ccgctaacac
     8881 cagaatgagt aagcagggcg cgaatgacag ccatgcagca ttgccacagt tctgccatat
     8941 cgcgctctgc ccaacctggc tgcgggctta atgcgcacag cggcaggcgc tgcacgcctg
     9001 cctcccggcc ttcgcggtca tacagcccgg ctttcagcca gctaccgcca caatctaacc
     9061 ccagccagta ttgcgtcatg gtttattgca cctcatccac ggctttcagc agctcagcgc
     9121 cctgcggaac atctttaaca aacatgtcgc ggacctgagt acccagtgcg tcgctaaagg
     9181 ctttgcggtc gagatcggtg atgacttcca cgcccgcttc tttcatgccg tcgatgattt
     9241 tttgctgatc ttcggcaacc agtttgcgct ggtagttacc cgcttcctgc gcagatgaga
     9301 tcagcgcctg ctggaactct ggggttaagc cgtcaaactt cgctttgttg atcaccacca
     9361 gaagcgggga ataggcgtga tgggtcagag aaaggtattt ctgcacttcg aaaaattttg
     9421 ctgaccagac gacgttgata gggtgttcct gagcgtcgat agtgcgggtt tccagcccgg
     9481 tatagacttc ggcaaacggc atcgggatcg ggttagcgcc aaagactttg aatgcggcga
     9541 tattcatcgg gctattgttg gtgcggattt tcagcccttt caggtcagca ggtgttttca
     9601 ctggcgcacg cgagttggtg acatcgcgcc agccgttttc ccaataggcc agtactttca
     9661 gtcctttacc ttcaagggag actttcaggt catcaccgac tttgccgtcg agcgttttat
     9721 gcgcgtgggc ggtatcgcgg aacaggaaag ggacatcaag caggttcatc acaggtgaca
     9781 acccggcaaa gttattcgag ccggacattt ccatatcgat ggtgccgcca cgtacgccgc
     9841 tgatcatcgc ctgtgcgtta ccgagagtac tgtccgggaa cagttttaat ttcagctcgc
     9901 ctttggttct ctcctgcaat aaatcattga attttttcgc cgcaatatgt tgcgagtcgg
     9961 tttgtgaggt ttcataaccg aaacgtaaag attgcgccgc cagtgaagat gtactaaatg
    10021 cagccaggcc agcaatgaat aatgcgtagg ttacagagcg taatttcata atattttcct
    10081 tttaattaat ccatttcaaa gggaggatga tgagatcggg aataaaaaca aatacgacta
    10141 ataatgaata taaaaccagg acgtaaggga aaacgcctct gactgcatca tcgaatttga
    10201 gttttgccac ccctgaaata acgttaagca cattaccgat aggcggtgta attaagccga
    10261 ttgagcagtt aatgataaac atgacaccga aataaatcgg atcgattcct gcctctttaa
    10321 ctaaaggcat taataccggg gtaagaatta ataccgtcgg cgttaaatcc atgaccatgc
    10381 cgacaatcag gatagccacc ataatgacga taaacagcag acgcggtgaa tcgaccagcg
    10441 gttgcagtaa atcagaaacc atcatcggca gttcagcaat ggtgatcagc caggcggaaa
    10501 cttgtgccga ggctaccaga aacatcacca ctgaggtcgt tttcgctgcg ccaatgagca
    10561 catgccaaag tgtggcgaag gtcatttcac ggtaaataac tgtggcgaca aacagcgcat
    10621 agaaagcagc aacggccccg gcttcggtag gggtaaacag accggagcga aacccgccaa
    10681 taatgatgac tggaagaaac agcgcccaga taccggagac aaaagagtgc cagatttcct
    10741 gcatcgttgc tttttgctgg cggggtaaat taaggcgact ggcctgccac caccaggtaa
    10801 gcatcagcgt tgcgcccatc atcatgccag gggcaatgcc cgccataaac agcttactga
    10861 tggataatcc gctggaaacg ccgaaaataa taaacggaat ggaaggggga ataattggcg
    10921 cgataatgcc gccagaggca atcagccccg cagcccggtt gaccgggtag ttagcgctgc
    10981 gcatcatcgg cactaatagc gcggcaacgg cggcggtatc agcaacagca gaaccagaaa
    11041 ggctcgccat aatcatagcc gccagcacgc cgacgtagcc caacccgcca ggtttatgcc
    11101 ccaccagttt catcggcagg tcaacaatgc gttttgacaa gccgcccgca ttcatgattt
    11161 cacccgccag cacaaagaac ggaatcgcca gcagggagaa gctgtcggct ccgttcacca
    11221 gcgtttgtgc catgatctgg acatcaaaca tgtccagcca gaacattaac gccgctccgc
    11281 acaacaacag tgcccaggca ataggcaaac cgatagcaat accacccaac agacagccca
    11341 gaaaaatcag cacagccatg attaagctcc ttgcggtggc gacgttagag aattgctacg
    11401 cgtgatgagt tgatataaat gacgcagttc aaagaatgcg ataacgaggc tggtgggcag
    11461 acaagcggca tacatcaggc cgatgggcaa accgaggatc ggtgaataat cgctccagtc
    11521 ctgaattgtt tttagcgttg cgccccaggc cagtgcgcca caaataaata agattaagga
    11581 atgggtaacc agagcgactc gtcgctgcca tgcgggggag agtttctcca ccagaaaggt
    11641 gacctgaacg tgggcgttat ccataaaagc cacaatcgcg ccaataaatg ttaaccagac
    11701 aaataaataa cgtgacaatt catctacaga taaaatgctt gtctgaaaac catatcttaa
    11761 aataatgttt ataaacacaa tacaggaaag tacggcgaga ttaatcgcca gtattgcttc
    11821 aagtattttt ttcatagcta ttccttgagg ctatgtctgt cataattcaa gttgcatgtg
    11881 cagcaaccta aattattaat atagttagag caataacatt acccaggcgt tctggcgtag
    11941 acgattattc gattaattca gcgctgttaa tgcgacttta accacaattt ttcgtatctc
    12001 agaggttgtt tgcagaatac aacctggtcg gtgaacatcc tgcgggaaga aaatagcgta
    12061 gctgcccggt atcatttcta taaatgattc atgttcacta tcgtgataaa aaataatatc
    12121 gcgctgctct aatagtgatt cgctgacttt attatttccc gtatcaatag caatgccgat
    12181 tttctcttca ccccacgcca gaaactgaat atcgatatac cgacgatgca cttccggacg
    12241 gttttccgcc gcttcgcgtg tggttaaatc gataatttgc gcataaatat tcttgccgtc
    12301 gatttcgaca acgcccggtt ccagggcgtt gaagtcggtg gtgcgcagaa aatcaagcgc
    12361 cttttcaatg gcggcaggca aacggcacgg gtcaggctgc gcgatatgtc caaaaatcat
    12421 gggttatctc cttataacgc ttggattttt gcccacacgc tgtcatcaac ggtaataccg
    12481 ttacggcggt tttcggccag cagggtagta aattcgtggc cgggtaagcg aatggcctga
    12541 ttttcgtcag cgcgttcagc ggtggtaacg taatccatga tgcgttgcag tttggcatcg
    12601 cgggtggggc cgtcgataag cttatctact tcaatggcaa taaaaatttg tgaaatgccg
    12661 tattcgtcgc tgttgtcctg ggtgacttcg gcaaccgatg cgccgtcaga aaggagagtg
    12721 gcgatcatat ccagcacaat cgacatgccc gaacctttcc agtaacccat cggcaaaatg
    12781 cggcgattct tctcgataac gccaggttct ttggtcaaat tgccctcatc atcaaagcca
    12841 ccatcgaccg ggagctgacg gcctgccaga cggttaactt ctaacatgcc gtaagagaac
    12901 atcgacatcg acatatcgac catggtgatt ggggtagaag gaatggcgac gatcagcggg
    12961 ttagtgccga tgcgacactc ttttgcgccc cacggcggca ttacggcgat ggagttggtc
    13021 cagcaaatgc caatatagcc tttttccgcc gcctgccagc cgtagctgcc gccgcgcatc
    13081 cagtggttgg cattacgtag tgccaccaga ccaataccgt gatcggcagc cagttcaatg
    13141 gcgcgatcca tcatcttttt cgctgtcagg ttaccgatcg aacgctgggc gtcccactgt
    13201 tcaattgcgc cgaggctggt tatacgtttg ggttgggcat caggaatgat atcgccgttt
    13261 tccagttgtt gaatgaaacg agggaaacgg ttgacgccgt gagaataaac gccggattcg
    13321 gtggtgcggg cgaacatttc ggcacaggca tcagccgttt cgctgtcaac gtcgcgtgaa
    13381 attaagactc gattaaaggc tgcttttaac tgctcaaatg tcactttcat ccctggcttc
    13441 cttgtttttt tgatcgcttt tttggcttaa aatttcaata tgcgaaatct gatttcaaat
    13501 atatcgatcg ctttttaaca aagcaatctg attgatgaat ttcaaaaaac ttaaaaatca
    13561 agtggttata aattatctgt cgagatcgtg aactacgaca cactttgcgc taccatcttg
    13621 acgcgacaaa atggggaaag aagtgatggg aaaaaaagag aacgagatgg cgcaggaaaa
    13681 agagcgtcca gccggaagcc agagtctgtt tcgcgggttg atgctgattg agattttgag
    13741 caactatcca aacggttgtc cgttggcgca tctttcggag ctggctggtt taaataagag
    13801 taccgtccat cgcttattgc agggattaca atcctgcggc tatgtgacca ccgcgcccgc
    13861 cgcagggagt tatcgcctga ccaccaaatt tattgccgtc gggcagaagg cgctgtcttc
    13921 gctgaatatc attcatatcg ccgctccgca tcttgaggca ctgaacatcg ccactggtga
    13981 aactattaac ttctccagcc gcgaagacga tcacgctatt ttgatttata agctggaacc
    14041 cacaaccggg atgctgcgaa cccgtgccta tattggccag catatgccgc tctactgttc
    14101 cgcaatgggc aagatctaca tggcgtttgg ccacccggac tacgtgaagt catactggga
    14161 aagccatcag catgagatcc agccgttaac ccgcaatacc attaccgagc tgcccgcgat
    14221 gttcgacgaa ctggcgcaca ttcgtgaaag cggagcggcg atggacagag aagaaaacga
    14281 actcggcgtc tcctgtattg ctgttccggt gtttgatatt catgggcggg tgccgtacgc
    14341 cgtgtcgatt tcgctttcga catcacgtct gaaacaggtg ggggagaaaa atctcctgaa
    14401 accactgcgt gaaaccgcgc aggctatttc taatgaactg ggatttactg tcagggacga
    14461 ccagggcgcg atcacataac gctttggaca agtgccaaaa ctttaacatt tccttcgttg
    14521 gatcaaagca gtagagacgc gctctctggc actctgctgt tttagtgcaa aggagtgatc
    14581 atgaaccggt ttattattgc ggatgcgacg aaatgtatcg gttgccgtac ctgtgaagtg
    14641 gcttgcgcag tgtcgcatca ggagaatcag gattgcgctg cgttgtcacc agacgagttt
    14701 atttcccgta ttcgtgtcat taaagaccac acatggacca cggctgtagc ctgccatcag
    14761 tgtgaagatg caccgtgcgc gaatgtctgc cctgttgacg cgataagccg cgaacatggg
    14821 catattttcg ttgaacaatc acgttgcatt ggctgtaaaa gctgtatgct ggcttgcccg
    14881 tttggtgcga tggaggtcgt ttcttcgcgc aaaaaggcga gggcgattaa gtgcgatctg
    14941 tgctggcatc gggcgacggg accggcctgt gttgaagcct gcccgacaaa ggcgttgcag
    15001 tgcatggatg tcgagaaagt gcagcgacat cggctacggc agcagcctgt ttgaaacgtt
    15061 tttccggcgt ttctcaacgc agggatagtg cgctttgttt atgccggatg cggctaaaac
    15121 gccttatccg gcctacaaat tctaccaaat tcaatatatt gcagaaatca tgtaggcctg
    15181 ataagcgtag cgcatcaggc aatttttagt gactttcagc ccaggctctt tctatctctt
    15241 ctgccagaat cttcacaccc gcctcaattt tctccggctc cggcacgtag ttcatgcgca
    15301 tacattgatg tgtatgtggc cacggtttat ccagccctgg gaagaagttg tgccccggca
    15361 ccatcagcac gccgcgtgct ttcaggcgct gatagagctg ctcggtcgta atgggcaaat
    15421 ccttaaacca tagccagagg aaaatggctc cttccggttt atgaatcagg cagcgatctt
    15481 ccggtaaata gcggcgaatg atggcgatag tttcctgaac acgctggtag taaaacggtt
    15541 tgatgactgt ttcagacagg cgcagcagat cgttacgctt aatcatttca cacatcatcg
    15601 ccggaccaat accgccaggt gccaggctga taatgccgtt catattggtg atggcggtga
    15661 tgattttttc attggcgatg ataatgccgc agcgggagcc aggtagaccc agcttggaaa
    15721 gactcatgca cagtacgata ttcggattcc acagcgggcg cgcttcgctg aagatgatac
    15781 ccgggaacgg gacgccataa gcgttatcaa tcaccagcgg aatgccatgt tgattcgcca
    15841 gcgcgtcaag cttcagcaac tcttcgtcag taatcacatt gcctgttgga ttcgtcggcc
    15901 gggagacgca aatcatcccg gtttcttcgc caatatgcag atgctcaaaa tcgacgtggt
    15961 atttaaactg gccttccggc agcagttcaa tattcggacg cgcagagaca aacagatctt
    16021 cttccagtcc ggcgtcagcg tagccaatgt attccggtgc cagcggaaac agcacttttt
    16081 tgacccgacc atcggcacgg cgtccggcga aaagattaaa taagtagaaa aacgcgctct
    16141 ggctgccgtt tgttagtgca atattctgtg gttcgatatc ccaacccaac ttctcgcgca
    16201 gcattccggc aagcagtgtg agtagctccg ttttcccctg tggaccgtcg taattgcaca
    16261 gtgcatcagt cgctttgcca ctttccagca tgtcggtcag tagcgtctgg aagtagtcct
    16321 gcatttccgg gatctgcgcc ggattaccgc cgccgagcat aatcgcgccg ggcgtgcgta
    16381 aaccgtcgtt cagatcttcc atcagcagcg taatgccgga gtggcgggta aatttgtcac
    16441 caaaaaggga gaatgtcata gcgggatatc tgtcgaactc tgtagcaagg aaggtaacaa
    16501 taacgctaca tttgctgggg tgcaaatcgc tctgttgaag agggagcggt gctttatcct
    16561 gcaacgctga ttagagctga cattttatcc tggttggcgg cttcccgagg tactgacgaa
    16621 cgggaagccg gaaaagtcac tgttgcccag cccagatgac cagcacttta tcgtcgccat
    16681 gctcacgaac aaagccgtag ccctgcttca gtgaaagtgt cgtttgtttg ccctcgccaa
    16741 ttgcgggatg gcgggcgcgg aactggctga ttttctgcca gtgcgcgacg ctggcggcag
    16801 atttaccgct aacatcctgc cagttcatat ccgaacgcgt accttgcagc ggatccgaac
    16861 cggtggggcc gaacggacgc gaggattcat caccataaaa gatttgtacc gcgcctggcg
    16921 ctaataataa taactctgct gctttgtcgc ctccttcacg gaacaggcgg gtatcatgcg
    16981 acgagaggta gctcaacacg ttgaaatcct gcaatttctc cgccatttgc tgccaggtcg
    17041 tatccatctg cgccagacaa tcgactgctt tagccgcctg ctcctgataa tcgaaattga
    17101 tcatcgcatc gaagccgtgg cgatagtagt cactttgcat cacgccgtgg ccccaggctt
    17161 caccggtcat ccagaacggt ttgtcatcta atgttttgtc ggggttagct tttttccatt
    17221 cgcgaagtgc ggcgctggct tcggttttca gttgctgcca ggcgggcaac tcaacatgtt
    17281 tggcggtgtc gacccgaaaa ccatcaatcc catagtcgcg aacccactga cttaaccagt
    17341 gggttaagta atcgcgaggg gtaaaaccgt cgatgacttt agcgtgggta tccgttttgt
    17401 ttttatagaa caccggcaga ccagaagcgg tcgttgattc ggttttgata tccggcaaaa
    17461 aggccagcga catagtgaga tcgtcgaatc caggattgtc gtaatcgccg atatcggttc
    17521 tgatccagtt ttttccccac catttatccc agcctgtttt gtcgctgaaa ttaatgtaat
    17581 cgttaaagct atgccaggtt tgcccggcgg caggtttcca gtcgctccag cgttcaccca
    17641 acgttttttt cacttcgtca ccagaaagat ataacgcgcc aaactgatac tcctgcatat
    17701 ccgccagtgt ggcatagccg gtgtggttca tcacgacatc aaagagaata cgaataccgc
    17761 gctgatgcgc gctatcaacc agtgtccgta gagcggcttc actgcccata ttggcatcaa
    17821 gattcgtcca gtcctgtgtg taataaccgt ggtaggcata atgcgggaaa tcgccttttg
    17881 taccgccgcc gacccagccg tgaatttgct caaatggggc gcttatccac aaagcattga
    17941 cgcccagctg ctggaggtaa tccagtttgt tggtcaggcc gcgtaaatcg ccgccgtgaa
    18001 aagtgccaat ttctgccata ccgtctttat gacgtccgta actctggtca ttactgggat
    18061 cgccgttttc gaaacgatct gtcagcacaa agtaaaccgt ggcgttatgc cagtcgaaag
    18121 gggcaggggc gtcagtttct gcccgttcca gcaggagtaa accgttgctg gtggcagcgg
    18181 gttgtaacgt tatttgaccg ttcttcacta tcgcaatttg ctggctgtaa taatcccgga
    18241 ctgcggctcc ctccggaaaa gtggcgctga catccagtgt gagcggtaat ccatcccatt
    18301 tcgggcattc acggaccagg cttgctaccg gttcgacggc gttctggatg gaaatcatca
    18361 atgttggtgt accggagcgg gtgtctagtt gcagcgtata ttcgccatcc ctgaacaatc
    18421 gccattgagg tggcgtgttg ctacaaggtt gcagggaaag catctgattg agttttatcg
    18481 catccgcagg ctgccagcac tgttggtcaa aatttagcgt tagtggacgc gtacctttgg
    18541 gcaactgcgc gtggctgaca aatgttcccg tcccctgttc gctaaaggcg ggaaaccccg
    18601 gagaagtcca gctggcggca acggcgaagc caggaaggag tgtcagaaaa caggcggcga
    18661 gtttcatggc ggatgagtcc ttattgctgc gatttcagac agtttgccag cgataagcca
    18721 gacaaaactc atccctgagc ggtaagtcca caggatgaga agcaagggtg agcgatcgcg
    18781 cacaaaaaac ggctgaattt tgcgataaac cccacatttt ctgcgattta gcgccaatct
    18841 gaatcgttaa ctcgtgacat agtttcagat ttggactatt ccttggggtg ctcttgacag
    18901 ctatttttga tatgatttga gattcaactc tcaaatttgt gaaaaatata aggtgttgga
    18961 atgattaaat ccgaccagga gacctgatga ttttgactcc catacgacga tatggggcga
    19021 tgattcttat gttactcact ctggtgtttt cgagtgaggt gttagcgaag acgcacacaa
    19081 caacagcgag tcaaaagtcc cacttaacta aagctagtaa taaacaggta agcagtaaac
    19141 aagagtattc tcgcaatagt gcaaagagta gttcacttcc tgatttgcga aaataccctt
    19201 ccggaacacc aaggaaaaag gcgtttctcc ggaccgtaat gccttacatt accagccaaa
    19261 atgcagccat tactgcggaa cgtaactggc tgatttcaaa acagtatcag ggccaatggt
    19321 cacctgctga gcgtgcgcgt ctgaaagaca tcgccaaacg ctacaaggtg aaatggtctg
    19381 gtaatacgcg aaaaatcccg tggaatacct tgctcgaacg cgtagacatt atccccacca
    19441 gtatggtggc gacgatggct gcagcagaaa gcggttgggg aacgtcgaag ctggcgcgca
    19501 acaacaacaa tctgttcggc atgaaatgca tgaaaggacg ttgtactaat gcgccgggta
    19561 aagtgaaggg gtactcacag tttagttctg tcaaagaatc ggtgagcgcc tatgttacta
    19621 acctgaatac gcatccggct tactcttcgt tccgtaaatc gcgtgcacag ctgcgtaaag
    19681 cggatcagga agtgactgcc acagcgatga ttcacaagtt gaagggctac tcgaccaaag
    19741 ggaagagtta taacaactac ctgttcgcaa tgtaccagga taaccaacgg ttaatcgcgg
    19801 cacatatgta attgcatttc ctcgagcctt atccgacttg tcagtcggat aaggcttttg
    19861 ctcgcatcag gtggttgtgc tgagttacct gatgcgacgc cgacaattct catcatcgct
    19921 acaacatgac ctcgctattt acatcgcgat actcttttgg cgtcgtgtca tatgcttttt
    19981 taaaaacaga gtagaaatat tgcagcgatg gataaccgca catttgcgat atctcattga
    20041 tcgacaaggt ggttgaaatc agcagactgc gcgctttctc cagcttctcg gcatgaatca
    20101 tggcatggat ggtttcaccc acctcttctt taaaacgctt ctcaagattg gagcgtgata
    20161 tgcccaccgc atcgagtacc tgatccactt taatcccttt acaggcgtga ttacgaatgt
    20221 aatgcatggc ctgaataacg gcgggatcgg tcagcgagcg gtaatctgtt gagcgccgtt
    20281 caatgacgcg aactggtggg accaaaatcc gctgtagcgg catttcttct ttatctaata
    20341 atcgatgcaa cagttttgcc gcctgatagc ccatttgccg cgcgccctga gcgaccgaag
    20401 aaagggcgac acgcgacaga tagcgggtca gttcttcgtt atcgatgcca atcacgcata
    20461 atttttccgg tacgggaata tgtagatgtt cacatacttg cagaatatgc cgcgcccggg
    20521 cgtcagtaac ggcaataatc ccggtttgcg gtggcagcgt ttgtagccag tctgccagcc
    20581 gattttgcgc atgttgccag ttctctggcg cggtttctaa cccctgataa accactccgc
    20641 gatacttttc ttcggcgaca agctgacgaa atgcatattc gcgttcagtg gcccaacgtt
    20701 tgccgcttga ttccggaaga ccataaaaag caaagcggtt aacgcctttc tcttttaaat
    20761 gcaaaaatgc gctttcaacc agcgcatagt tatcggtggc aatgtaatga acgggtgggt
    20821 aactttctgc aaggtgatac gagccgccaa ccccaacaat agggacgtgg acatcagcca
    20881 gcgcttgctc gatctgtttg tcgtcgaagt cggcaatgac gccatctccc aaccagtcct
    20941 tgattttatc aatgcgggcg cggaaatctt cttcaatgaa aatatcccat tccgattgtg
    21001 acgcctgtaa atattcccct acgccttcta ctacctgccg gtcataggct ttattggcat
    21061 tgaacagtaa tgtgatgcgg tgacgtttag taaacatggt tcttttcctg ctgaatcatg
    21121 caaaaactca aaaccggtaa tacgttaccg gctttgagaa aatttttatc aaaatcaaga
    21181 acggcgtttg gttgcggagt ccatccatac tgccagcaac agaatcgcac ctttaacgat
    21241 atactgccag aaggtcggta catccatcat actcatgccg ttatccagtg aagccatgat
    21301 gaatgccccc attaccgctc cggcaacgct tcccacaccg ccagccagac tggtgccgcc
    21361 aatcacgcat gcggcaattg cgtccagttc ggcgatattt cccgcagaag gtgaaccagc
    21421 gccaagtcga gaactgagga ttaatccggc gatagcgacc attaatccgt taatagcgaa
    21481 tacggcaagt ttggtgcgtt caacgttaat cccggagaga cgtgctgctt ccagattgcc
    21541 gccgatggca taaatgcgtc gtccaaatgc cgtccgcgtt gccataaaca ttccgccgag
    21601 taacagcaac gtcagcagca gaacgggagt gggaacgcca cggtaatcgt tcaacagcca
    21661 gattgcgcct aagacgataa tcgccgttaa tgactggcga ccgacaactg cggtagaggc
    21721 cggagactgc aaacccaaag cctgacggcg cattcttccg cgccattgcc agccaacaaa
    21781 agccattaag ccaagcgcgc caatgatgaa gccggtgctg gcgggtagat agctttgccc
    21841 aatttgtgac atcgcggcgc tggtggggga tacagtcgtg ccgttggtga tgccaatgag
    21901 tatgccgcga aatgccaaca tgcccgcgag ggtgacaata aatgaaggga ctttgcggta
    21961 cgctacccac catccgttcc aggcaccgag aagcagtccc agaaccaaag tcacaatgat
    22021 ggtcagtggc aaaggccagc ctagccagac gtcacaaatc gccgcgacgc cacctaatag
    22081 ccccatcatt gagccgacgg aaaggtcgat ttcagcagaa attatgacga acaccattcc
    22141 tactgcgagg atgccggtaa tcgcggtctg gcgtaacagg ttggagacgt tacgggcgct
    22201 taagtaggca ccatcggtgg tccaggtaaa gaacagcatg attgcgatga tagctgcaat
    22261 catcacgaag acctgcaaat tcagtgactt cagcccggaa aagccaccgg atgtcggtac
    22321 ggccaatttc acttcagacg gattgctttt cgacatgatg ttcgctcctc aatgcggctt
    22381 ccatcacctg ctcctgagtc aggttatgat ttatcaggtt ggcttttagt ttcccttcat
    22441 gcatcaccag tacacgatcg ctaaggccga gcacttcagg taattcggaa gagatgacaa
    22501 taacggcaat accctgctgg acgagttggt taattaattt gtagatctcg tatttcgcgc
    22561 caatatcgat acccctggta ggttcatcaa gaatgagaat gcgcgggttg agtaacagac
    22621 agcgagcgag gatcgctttt tgttgattgc cgccgctcaa acatccaata gcaaggtcgg
    22681 gggacgacgt tttaactttg agttgctgga ttgattccag aatacatttt tgctctgccg
    22741 cgtcatcaag ctggctaata ccgccggtaa atttattgag tgcggcgagg gtaatatttt
    22801 taccaaccgc cattaccgga acgatgccgt cgcgctttct gtcttcgggg accatcgcaa
    22861 tcccctgggc gatggcttgc tgacagttac gaatatctac ctgtttgccg tcaatataaa
    22921 tttttccttc ccattgtccg ggccacacac caaacaggca ctgaatggtc tcggtacgtc
    22981 cggcaccaac gagtccggca atacccagta tttcgccacg tttcagggaa aacgagacat
    23041 cattaactcg tttaatatga cgattaaccg gatgccatgc cgtcagatgt tcaatacgta
    23101 atatttcatc tccggtggta tgtggttcat ttgggtaaag cgcggttaac tctcgcccga
    23161 ccatcatggt gataatatcg tcttcactca ttccggcagc atcacgcgta ccaatgtgct
    23221 gaccgtcgcg aataacgcaa atcgtatcgg aaatcgcttt gacttcgttg agtttatgcg
    23281 aaatataaat acaggcgata ccgtgctgtt gcagatcgcg aataatatcc agtaaaaccg
    23341 acgtttcctg ctcagttaat gaggctgtcg gttcatcgag aattaacaaa cgcacctgtt
    23401 tattaagtgc cttggcaatt tcaaccagtt gttgttgccc aagccctaaa tcgccaacgc
    23461 gggtatcggg tgaaatggat aaactgacct gtgcgagcag cttctgacag cgtagcgtca
    23521 tcaggtcata atccataatg ccattgtggg ttatttcgtt acccaggaag atattttcca
    23581 gcacggtcaa ttctttcacc agggccaatt cctggtgaat gatggcgata cctttgcgtt
    23641 cggtatcgcg gatgtgactc gcctgaatct cttctcccgc aaaaataatt tcgccttcgt
    23701 aggagccatg gggataaata ccacacagca ctttcatcag cgttgattta ccagacccat
    23761 tttccccaca gagtgagacg atttcgccag cattcaaccg caagctgacg ttatcaatcg
    23821 ccttcacact gccgaaggtt ttggtaatgt tcttcatttc aagtagataa ggcataacga
    23881 ctccacctaa gccaattcat tcacgcggca tggagagaaa tcacgccccc gctctgcgcc
    23941 ggggcgtaac gcttacagct cgctctcttt gtggaatccg tctttaacta ccgtgtcttt
    24001 gatgttgttt ttattcacat cgatcggtgt cagcaggcgg gagggaacat ctttcaggcc
    24061 attattcagt gtggtatctg cttttggttc ctgaccattg cccaactcaa cggcaatttc
    24121 tgcggcagta tttgccaaca acgtaatagg tttatacacc gtcatagttt gcgtaccggc
    24181 agcaatacgt ttaatacctg cgagatccgc atcctggccg gaaattgcta ctttccctga
    24241 taaaccttgc gcgcttaatg cctgaattgc cccacctgcg gtggcatcgt ttgaggcaac
    24301 tacagcatca attttgttat tattggcggt tagcgcgttt tccataattt tcaatgcgtt
    24361 ttccggtaac cagccatcaa cccattggtc accaacgact ttaatttttc cagaatcaac
    24421 gtaaggtttt aacactttca tttgtccggc gcggaacagc ttagcgttgt tatctaccgg
    24481 cgagccgccc atcaggaagt aattaccttg cggaacaata tcgaccaggg cttttgcctg
    24541 cagttcaccg actttttcgt tatcgaaaga aatataaaaa tcgatatccg catcgttaat
    24601 catacggtcg taagctaata ctttaatacc ttcttgtttg gcttctttta caacgttact
    24661 taatacctga ccgttataag gaataatgac aagaacatcg acaccccggt taatcatgtt
    24721 ttcaatctgc gacatttgtg tttcttcatt gccatttgca gactgtacaa atactttcgc
    24781 gccgagagat tctgcttttt tcacaaaaat atctcgatct ttttgccagc gttcaagacg
    24841 gagatcatca atcgccatac ctattttgac ttctttggcg tgtgccgcaa cgttggtaag
    24901 cagaagtgag gtgcaaaggg tgagtagaat gttctttatt tttatggtgt agggccttct
    24961 gtagttagag gacagcttta ataagtaaca atcaccgcga taaacgtaac caatttttag
    25021 caactaaaca ggggaaaaca attacagatt tttatctttc gattacgatt tttggtttat
    25081 ttcttgattt atgacagaga tcttactttt gttgcgcaat tgtacttatt gcatttttct
    25141 cttcgaggaa ttacccagtt tcatcattcc attttatttt gcgagcgagc gcacacttgt
    25201 gaattatctc aatagcagtg tgaaataaca taattgagca actgaaaggg agtgcccaat
    25261 attacgacat catccatcac ccgcggcatt acctgattat ggagttcaat atgcaagcct
    25321 attttgacca gctcgatcgc gttcgttatg aaggctcaaa atcctctaac ccgttagcat
    25381 tccatcacta taatcccgac gaactggtgt tgggcaagcg tatggaagag cacttgcgtt
    25441 ttgccgcctg ctactggcac accttctgct ggaacggggc ggacatgttt ggtgtggggg
    25501 cgtttaatcg tccgtggcag cagcctggtg aggcactggc gttggcgaag cgtaaagcag
    25561 atgtcgcatt tgagtttttc cacaagttac atgtgccatt ttattgcttc cacgatgtag
    25621 atgtttcccc tgagggcgcg tcgttaaaag agtacatcaa taattttgcg caaatggttg
    25681 atgtcctggc aggcaagcaa gaagagagcg gcgtgaagct gctgtgggga actgctaact
    25741 gctttacaaa ccctcgctat ggcgcgggtg cggcgacgaa cccagatcct gaagtcttca
    25801 gttgggcggc aacgcaagtt gttacagcga tggaagcaac ccataaattg ggcggtgaaa
    25861 actatgtcct gtggggcggt cgtgaaggtt acgaaacgct gttaaatacc gacttgcgtc
    25921 aggagcgtga acaactgggc cgctttatgc aaatggtggt tgagcataaa cataaaatcg
    25981 gtttccaggg cacgttgctt atcgaaccga aaccgcaaga accgaccaaa catcaatatg
    26041 attacgatgc tgcgacggtc tatggcttcc tgaaacagtt tggtctggaa aaagagatta
    26101 aactgaatat tgaagctaac cacgcgacgc tggcaggtca ctctttccat catgaaatag
    26161 ccaccgccat tgcgcttggc ctgttcggtt ctgtcgacgc caaccgtggc gatgcacaac
    26221 tgggctggga caccgaccag ttcccgaaca gtgtggaaga gaatgcgctg gtgatgtatg
    26281 aaattctcaa agcaggcggt ttcaccactg gcggtctgaa cttcgatgcc aaagtacgtc
    26341 gtcaaagtac tgataaatat gatctgtttt acggtcatat cggcgcgatg gatacgatgg
    26401 cgttggcgct gaaaattgca gcacgcatga ttgaagatgg cgagctggat aaacgcatcg
    26461 cgcagcgtta ttccggctgg aatagcgaat taggccagca aatcctgaaa ggccaaatgt
    26521 cactggcaga tttagccaaa tatgctcagg aacacaattt gtctccggtg catcagagtg
    26581 gtcgccagga gcaactggaa aatctggtaa atcattatct gttcgacaaa taacggctaa
    26641 ctgtgcagtc cgttggcccg gttatcggta gcgataccgg gcattttttt aaggaacgat
    26701 cgatatgtat atcgggatag atcttggcac ctcgggcgta aaagttattt tgctcaacga
    26761 gcagggtgag gtggttgctt cgcaaacgga aaagctgacc gtttcgcgcc cgcatccact
    26821 ctggtcggaa caagacccgg aacagtggtg gcaggcaact gaccgcgcaa tgaaagctct
    26881 gggcgatcag cattctctgc aggacgttaa agcattgggt attgccggcc agatgcacgg
    26941 tgcaacctta ctggatgccc agcaacgggt attacgccct gccattttgt ggaacgacgg
    27001 gcgctgtgcg caagagtgca ctttgctgga agcgcgagtt ccgcaatcgc gggtgattac
    27061 cggcaacctg atgatgcccg gatttactgc gcctaaattg ctatgggttc agcggcatga
    27121 gccggagata ttccgtcaaa tcgataaagt attattaccg aaagattact tgcgtctgcg
    27181 tatgacgggg gagtttgcca gcgatatgtc tgacgcagct ggcactatgt ggctggatgt
    27241 cgcaaagcgt gactggagcg atctcatgct gcaggcttgc cacttatctc gtgaccagat
    27301 gcccgcatta tacgaaggca gcgaaattac tggtgctttg ttacctgaag ttgcgaaagc
    27361 gtggggtatg gcggcggtgc cagttatcgc aggcggtggc gacaatgcag ctggtgcggt
    27421 tggtgtggga atggttgatg ctaatcaggc aatgttatcg ctggggacgt cgggggtcta
    27481 ttttgctgtc agcgaagggg tcttaagcaa gccagaaagc gccgtacata gcttttgcca
    27541 tgcgttaccg caacgttggc atttaatgtc tgtaatgctg agtgcggctt cgtgtctgga
    27601 ttgggccgcg aaattaaccg gcctgagcaa tgtccctgct ttaatcactg cagctcgaca
    27661 ggctgatgaa agtgccgagc cagtttggtt tctgccttat ctttccggcg agcgtacacc
    27721 acacaataat ccccaggcga aaggggtttt ctttggtttg actcatcaac atggccccaa
    27781 tgaactggcg cgagcagtgc tggaaggcgt gggttatgcg ctggcagatg gcatggatgt
    27841 cgtgcatgcc tgcggcatta aaccgcaaag tgttacgttg attggtggcg gggcgcgtag
    27901 tgagtactgg cgtcaaatgc tggcggacat cagcggtcag caactggatt accgcacggg
    27961 tggcgatgtg gggccagcac tgggcgcagc aaggctggcg cagattgcgg cgaatccaga
    28021 gaaatcgctc attgagttgt tgccgcaact accattagaa cagtcgcatc taccagatgc
    28081 gcagcgttat gccgcttatc agccacgacg agaaacgttc cgtcgcctct atcagcaact
    28141 tctgccatta atggcgtaaa cgttatcccc tgcctgaccg ggtgggggat aattcacatc
    28201 tatatatctc agtaattaat taatatttag tatgaattta ttctgaaaat catttgttaa
    28261 tggcattttt cagttttgtc tttcgttggt tactcgtaat gtatcgctgg tagatatgga
    28321 gatcgttatg aaaacctcaa agactgtggc aaaactatta tttgttgtcg gggcgctggt
    28381 ttatctggtt gggctatgga tctcatgccc attgttaagt ggaaaaggtt attttcttgg
    28441 cgtgttaatg acggcaactt ttggcaacta tgcatatctt cgcgcagaaa aacttgggca
    28501 actggatgat ttttttatcc acgtctgtca gttggtagcg ttaatcacta tcggtctctt
    28561 gtttatcggt gttttaaacg cacctatcaa tgcttatgaa atggtgatct accccatcgc
    28621 cttttttgtc tgcttgtttg gtcaaatgcg tttgtttcgc tcggcatgag caacataaag
    28681 ttcttacaca ttcaggaatg aaaggaatac tgtgatggac aacaaaatat caacctattc
    28741 tccggcattt agtattgttt catgggtagc ccttattggc ggtattgtta cctatctgtt
    28801 aggactatgg aatgcagaga tgcagttaaa tgaaaaaggg tattattttg ccgtactggt
    28861 attaggtttg ttttccgcgg cgtcttatca aaagaccgtt cgggacaagt atgaaggcat
    28921 acctaccacc cccatttatt atatgacttg cctgacggtc tttattatct ctgttgcgtt
    28981 gttgctggta ggtctatgga atgcaacgtt attactcagc gaaaaaggct tttatgggct
    29041 ggctttcttc ttaagcttgt ttggtgcagt agcggtgcag aagaatattc gtgatgccgg
    29101 aataaaccca ccaaaagaaa cacaggttac ccaggaagaa tacagcgaat aattcacata
    29161 agcccggtta gtccaatgta accgggcttt tactaactca ctaatctgtt tctgtcgatt
    29221 cgttgtacca gcatagaaag taacaaactc gctgccaacg tcgcgcaaaa gatccaaata
    29281 atatcgagta ttggccagtt tttaagctca atcccccggg tgcgcagtgc atggataatc
    29341 aatgcatgga acccgtatat acctaacgaa tggcgggaga ttaagccaag tccgcaaatg
    29401 gtacgcgtat acagcgtgtt tttaaccaga gtcaataacg cgattgcgca gataaaaacc
    29461 atcggcccac agtaaagata ccaggtatcg gcaaaatttc cgcgccactg taattcatat
    29521 aatgtcccgc gagagataat aaaaaccccc gtcgcaaaca gtgcggcgct cacccacgac
    29581 agtgctttat gctgtgtgtc catcatccct atagcgcggc ccaacatgcc ataaaggatg
    29641 tagtaaaaag tatcgccatt gatatataag ttaattggca gccattcaaa accgtcgatt
    29701 ttctgcggca cagtatttgg gttagcgatg ataccaatca ccaccattag caccagcaac
    29761 atttttccgc cgacgttctt cacctgaatc agcggtgaaa ccagataaat cactgcaatc
    29821 gcgaagaaaa accataaatg gtaaaacact ggcttttgca gcaggttttt cagcgctaac
    29881 tccacattga tggaggtaaa cagcgcaatg tagagcagtg cgattgcgct ataaaaaagc
    29941 agacataagc cgatacgcaa gaaatggcgc ggctgggcgc tgcgttcgcc aaaaaagaga
    30001 tagccggaaa tcatgaaaaa tagcggcacg ctgacacgag aggcagaatt cagaacattg
    30061 gcgatatccc aggtgacggg gctaacacta tgagcattgg tcacatacta ggtagtggtg
    30121 tgaatcatca ccaccattaa acacgctatc cctcgcaggt tatcaatcca gtaaattttg
    30181 ggctgcatct gtgtctctgt atctggttaa aaaaagtctg accgataaat cattcggttg
    30241 gcgcactgga ataatctgag ttttatcact acagcttata gaggcttaag gaaattcgta
    30301 agatatcagc cactataccg atataaataa taagactcac ctgcaaacca gacggtaatt
    30361 taatgatgat gaactctttc tttccggcaa tggcgcttat ggtgcttatg gcgctagtgg
    30421 gttgttctac accgccaccc gtgcagaaag cacaacgggt aaaggttgat cctctgcgtt
    30481 cgttgaatat ggaagcgtta tgcaaggatc aggcggcgaa acgttataac accggcgagc
    30541 aaaaaatcga cgtcaccgca tttgaacagt tccagggaag ctacgaaatg cgcggttata
    30601 ccttccgtaa agagcagttt gtctgttctt ttgacgcaga tggccatttt ttgcatcttt
    30661 ccatgcgtta agccctgctt tttcccgttt cgtactgtat atcttccatc cagcgggtat
    30721 actgatccct tcctttaaat ccacacgtat ccagcacgaa ataatatgca aaagtttgat
    30781 accaggacct tccagggctt gatcctgacc ttacaggatt actgggctcg ccagggctgc
    30841 accattgttc aaccattgga catggaagtc ggcgcgggaa cctctcaccc aatgacctgt
    30901 ctgcgcgcgc tggggccaga accgatggcg gctgcttatg ttcagccttc tcgtcgcccg
    30961 accgatggtc gctacggcga aaaccccaac cgtttacagc actactatca gttccaggtg
    31021 gtcattaagc catcgccgga caatattcag gagctgtacc ttggttctct gaaagagctg
    31081 ggcatggacc cgaccatcca cgacatccgt ttcgtggaag ataactggga aaacccgacg
    31141 ctgggtgcct gggggctggg ctgggaagtg tggctgaacg gcatggaagt gacgcagttc
    31201 acttacttcc agcaggttgg tggtctggag tgtaaaccgg ttaccggcga gatcacctac
    31261 ggtctggaac gtctggcaat gtacattcag ggcgtagaca gcgtttacga cctggtctgg
    31321 agcgacggcc cgctgggtaa aaccacctat ggcgacgtgt tccatcagaa cgaagtggag
    31381 cagtccactt acaacttcga atacgcggat gtggacttcc tgttcacctg ctttgagcag
    31441 tacgagaaag aagcgcagca gctgctggcg ctggaaaatc cgctgccgct gccagcctac
    31501 gagcgtattc tgaaagccgc ccacagcttc aacctgctgg atgcgcgtaa agccatctcc
    31561 gtcaccgagc gtcagcgcta tattctgcgc attcgcaccc tgaccaaagc agtggcagaa
    31621 gcatactacg cttcccgtga agccctcggc ttcccgatgt gcaacaaaga taagtaagag
    31681 gcggctatgt ctgagaaaac ttttctggtg gaaatcggca ctgaagagct gccaccaaaa
    31741 gcactgcgca gcctggctga gtcctttgct gcgaacttta ctgcggagct ggataacgct
    31801 ggcctcgcac acggcaccgt tcaatggttt gctgctccgc gtcgtctggc gcttaaagta
    31861 gctaacctgg cggaagcgca accggatcgt gaaatcgaaa aacgcggccc ggcgattgcc
    31921 caggcgttcg acgctgaagg caaaccgagc aaagcggcag aaggctgggc gcgtggttgc
    31981 ggtattaccg ttgaccaggc tgagcgtctg actaccgata aaggcgaatg gctgctgtat
    32041 cgcgcccacg tgaagggcga aagcactgaa gcactgctgc cgaatatggt tgcgacttcg
    32101 ctggcgaaac tgccgatccc gaaactgatg cgttggggtg caagcgacgt gcacttcgtg
    32161 cgtccggtgc acaccgtgac cctgctgctg ggcgacaaag ttattccggc taccattctg
    32221 ggcattcagt ccgatcgcgt gattcgcggc caccgcttta tgggcgagcc ggaattcact
    32281 atcgacaatg ctgatcagta tccggaaatt ctgcgtgagc gcgggaaagt catcgccgat
    32341 tacgaagaac gtaaggcgaa gattaaagcc gatgccgaag aagcggcgcg taagattggc
    32401 ggtaacgctg acttaagcga aagcctgttg gaagaagtcg cttcgctggt ggaatggccg
    32461 gttgtgctga ccgcaaaatt cgaagagaaa ttcctcgcgg tgccgtctga agcgctggtt
    32521 tacaccatga aaggtgacca gaaatacttc ccggtgtatg cgaacgacgg caaactgctg
    32581 ccgaacttta tcttcgttgc caacatcgaa tcgaaagatc cgcagcaaat tatctctggt
    32641 aacgagaaag tcgttcgtcc acgtctggcg gatgccgagt tcttcttcaa caccgaccgt
    32701 aaaaaacgtc tggaagataa cctgccgcgc ctgcaaaccg tgctgttcca gcaacagctg
    32761 ggtacgctgc gtgacaagac tgaccgcatc caggcgctgg ctggctggat tgctgaacag
    32821 attggcgctg acgttaacca cgctacccgt gcgggcctgc tgtccaagtg cgacctgatg
    32881 accaacatgg tcttcgagtt caccgacacc cagggcgtta tggggatgca ctacgcgcgt
    32941 cacgatggcg aagcggaaga tgttgccgtg gcgctgaacg agcagtatca gccgcgcttt
    33001 gccggtgatg acctgccgtc taacccggta gcctgtgcgc tggcgattgc tgacaagatg
    33061 gatactctgg cgggtatctt cggtatcggc cagcatccga aaggcgacaa agacccgttt
    33121 gcgttgcgtc gtgccgcact tggcgtgctg cgtattatcg ttgagaagaa cctcaacctt
    33181 gatctgcaaa cgctgaccga agaagcggtg cgtctgtatg gcgataagct gactaatacc
    33241 aacgtagttg atgatgttat cgactttatg ctcggtcgct tccgcgcctg gtatcaggac
    33301 gaaggttaca ccgttgatac catccaggcg gtactggcgc gtcgtccgac tcgtccggca
    33361 gatttcgatg cccgtatgaa ggcggtatcg cacttccgta ctctggatgc ggcagcgtca
    33421 ctggcggcgg caaacaagcg tgtatctaac attctggcga agtctgacga agtgctgagc
    33481 gatcgcgtga atgcttctac tctgaaagag ccggaagaaa ttaaactggc gatgcaagtt
    33541 gtggtgctgc gcgacaagct ggagccgtac ttcgctgaag gtcgttacca ggacgcgctg
    33601 gtcgaactgg ctgaattgcg tgaaccggtt gatgccttct tcgataaagt gatggtcatg
    33661 gttgatgaca aagaattgcg aatcaaccgt cttaccatgc tggagaaact gcgcgaatta
    33721 ttcctgcgcg ttgcggatat ttcgctgtta cagtaataac gccgttatta aaaagcctgc
    33781 catctggcag gcttttttat cgctaaataa tagagcaacc tttaataatc ttctgctgaa
    33841 taaagattgt ctcatatatt aattttatga gattttttta ggattatatc aaggagaaga
    33901 aacgaactta ttaagctaga atagctgtgg gtgcttgaga ctgtttgtct caggtattca
    33961 ccgaaaggca gacagagaaa agccccacct gactataaat caaagtgagg ctaccctatg
    34021 cctgaacacc ataaggttag cctcttactc gttggaaatc aacacaggag gctgggaatg
    34081 ccgcagaaat atagattgct ttctttaata gtgatttgtt tcacgctttt attttttacc
    34141 tggatggtaa gagattcact gtgtgaattg catattaaac agggaagtta tgagctggcg
    34201 gcatttttag cctgcaattt gaaagagtaa gagactttgg cgggaagtta ttcccgcctt
    34261 tcttacggcg ttgcgcattc tcattgcacc caaatttatt cttcacaaaa ataacactag
    34321 attctattac gcgatcgatt atttatttcc tgaaaaacaa ataaaaaaat ccccgccaaa
    34381 tggcagggat cttagattct gtgcttttaa gcagagatta caggctggtt acgttaccag
    34441 ctgccgggcc tttagcgccg ctttcgatgg tgaaggacac tttctgacct tcgtccagag
    34501 atttgtaacc atcgttctgg atagcagaga agtgtacgaa cacgtcttta gagccatcgt
    34561 caggagtgat gaagccgaag cctttgtcag cgttgaacca ttttacgata ccagtcattt
    34621 taccggacat agtgtattac ctttaataat taagtgtgcc tttcggcgat atggcgtgct
    34681 ttacagattt tgaagcgtta aaggaatgtg cactacgagg ggtatcaacg ataactcttg
    34741 aagggacttg ccttactaca ctgctttaat ggtctgtacg tcaaaccgtt gatgtgcatt
    34801 aagccacgca ttggcgggtg atgcaacaat tatttttcat atttatgatt aatcggttgg
    34861 aaaacggtcc tgtcatcagg accgtaaaca gcaataaagt ggataaaagt ctattccatc
    34921 aactgcttac ttaatgccgg gttggcttga atcaaacgca tcaattttag ttcggcactt
    34981 gaaggcttca cgcgtctgga ttcccattcc tttaccatgg cgactgatac gcccaaaacc
    35041 cgggcgaaat catcgatttt taaccctgtc ccttttcgta actgctcaat ttcggtacag
    35101 gacgttgttc tgtgcgtcag ggtaattttc tgcgtttcat ctttaaaaac aatctgttcc
    35161 aggctgctca acagctcatg cattggatct ttatattcca ttgaaaactc ctcaaatcac
    35221 actgcgggat cgtgaattgc atcgaagctc attaagaata gtcgggaaaa acagacatag
    35281 cggggtgtag tcagggcatg ctctggctat gattcgattc agccagtcat ttagcgtgac
    35341 ttatagccac ctaacgatct gataaatgcc gatatatctc ttaccactcc cggtttgtaa
    35401 agatttcgaa aatctaacgt tctgcgaagg cgtaaatctc aggctacaaa aaaagcaacg
    35461 gcactatcat tttttaacaa tgaaacaaca aattgacctt gtttcatttt ctccatgatt
    35521 ttaccaggaa gattacgtag aaagggtatc ctgcaagcct gtcctggact atccttgtca
    35581 cgtcagacac gcgtgtgtcg ttgtgcgctt tttttgggtg aaaggagtaa gaaaatggcg
    35641 acaggaaagt cctgctctcg ctggtttgcg cctcttgcgg cgttattaat ggtagttagc
    35701 ctgagtgggt gttttgataa agaaggcgat cagcgtaaag cgtttatcga cttcctgcag
    35761 aatacagtga tgcgtagcgg tgaacgtcta ccaaccctga ctgccgatca gaaaaaacag
    35821 tttggtcctt ttgtctctga ttacgcgatt ctgtatggtt attctcagca ggtgaatcag
    35881 gcgatggatt ccggtctgcg cccggttgta gacagcgtta acgcaattcg cgtaccgcag
    35941 gattacgtta cgcaaagcgg cccactgcgt gaaatgaacg gttctctggg cgtactggcg
    36001 caacagctgc aaaatgcgaa gttgcaggcc gatgctgcgc actctgcgct aaaacagagc
    36061 gatgacctga aaccggtctt cgatcaggca ttcaccaaag tggtaacgac gccagctgat
    36121 gcgttgcaac cgttgattcc ggcggcacaa actttcacgc aacaactggt gatggttgga
    36181 gactacattg ctcagcaagg tactcaggtg agctttgtcg caaatggcat ccagttcccg
    36241 acttcgcagc aggcaagtga atataacaaa ctgattgcgc cattaccggc acaacatcag
    36301 gcgtttaatc aggcctggac tacagcagtt actgcaaccc aataaagagt aaaagcccga
    36361 gcgattatcg ggctttactc cacgcagtcg cggcttagtc cgcgacgtgc ggattcacac
    36421 agttcttctc aacctttcct tgtaacgcat caatcaagtt atccacggca caggcggcca
    36481 tgccataacg cgtctcatga gtggcagatc caatatgcgg tactgcgacg acgttggcca
    36541 ttgagagcaa cggcgaatct actgacagtg gctcttgttc gaagacatcc agcccggcag
    36601 cgtgaatttc ccctttctgc aatgcagcaa tcagtgcatt ttcatcaacc accgggccac
    36661 gtccggcatt aatgaaaatg gcggaggatt tcattttggc gaattgttct gcgccaaata
    36721 gatgatacgt ctcatcagtt aacggcagga tcaggcaaac gaaatctgac tcttgtaaca
    36781 gtgtatccaa atcgcagtag cgggcgttga agcgttcttc tgcttcttta tggtggcggc
    36841 gcgcgttata gaggatgggc atgttgaagc caaagtgcgc acgttgtgcc agcgccatgc
    36901 cgatccgtcc catcccgaca atgcccagtg ttttatggtg aacgtcagtg ccgtaccagt
    36961 ccgggcctat gctcgcggtc cattcgcctg cttttactcg ttctgctacc tccacaaccc
    37021 gacgagcggt agacaatacc agcgccatca gcgtatcggc gacggtttct gttaatacgg
    37081 ttggcgtgtg catcagcaga atttttcggg cggtaagcgc atcgacatca aaattgtcat
    37141 agcctacgga gatcgttgat gtggcacgca gtttcggcat tttttccagc aatgcggcat
    37201 caacattctc gtttgaaccc agtaaacctt cagcttcggc aagaattgct gcattttgtt
    37261 cgatggtttg tgggctgagg tttgccacct ggtgaacggt gaaatgttct tgcaggcgtt
    37321 gcagtaaatc atcaggtaag gctttgtaga ggataacgga cggcttcatg cttctctcca
    37381 ttcactgata aggcgatcac cttagcctga aatatgatga aggaaaactg aagaatgcca
    37441 ggtgatttct gcatcacctg acatgaaagg gattacagtg ggcttaaggt aatttctaca
    37501 cggcggtttt gcgccttacc ttctgcggtg ctgttgctgg cgattgggtt agccgggcca
    37561 aggccctgag tacggatgcg gctggcgtcc acgccctggg tgatcaacgc gctggcaacg
    37621 gaatccgcac gctgctggga gagacgcatg ttcaggtcgt gaccccccgt gctgtcggta
    37681 taaccaatca cgttaaccgc cgttttcgga tactctttca gtaccattgc cacgccggtc
    37741 agggtgttag cgcccgccgg tttcagggtt gcgctgctgc tgtcgaaggt cacattgttc
    37801 ggcatattga ggataatgtt atccccgctg cgggttacgc taacaccagt gccgcgcatt
    37861 ttgtcacgca gcttcgcttc ctgcacatcc atgtaataac caacgccgcc gcccagagct
    37921 gcgcctgctg ctgcgccaat cagcgcgcct ttaccgcgat ctttcttcga agaagagagc
    37981 gcaccaatac ccgcgccaac gagagagccc agacctgcgc cgatagcaga tttacctgct
    38041 tcgcgttcgc cggtgtaagg gttagttgta cagccagata ccgccagagc gccactcact
    38101 acggcggcaa taagataaac acgtttcttc attgttaatc cttaataacc tttttattct
    38161 ttgccacggg ttctgtggcg ggagattatg ccgcgtgaac atgaagatta ttcctgggaa
    38221 tactcggaaa tttgtaagta atatttaact gctcaataca tctaaccttt caggagtctt
    38281 cggtttggcc aactcatcct cacgatattc cgttctgact gccgcccact gggggcctat
    38341 gctggttgaa accgacggcg aaaccgtgtt tagctcgcgt ggcgcgttag ccacaggaat
    38401 ggaaaactcc ttgcagagcg cggttcgcga ccaggttcac agcaatacgc gggtgcgatt
    38461 tccaatggtg cgcaaaggct ttcttgcgtc accggaaaat ccgcaaggca ttcgtgggca
    38521 ggatgaattt gttcgcgtga gttgggatga ggcgctggag cttattcacc atcaacataa
    38581 acgcattcgt gaggcttatg gtccggcatc gatttttgct ggttcctacg gctggcgttc
    38641 aaacggcgtg ctgcataagg cctcgacatt attacaacgc tatatggcgc tggcaggcgg
    38701 ttataccggg catctggggg attattcgac cggcgcggca caggcgatca tgccgtatgt
    38761 cgtgggtggc agcgaagttt atcaacagca aaccagttgg ccgctggtgc tggaacatag
    38821 cgatgtcgtg gtgctgtgga gtgctaatcc actcaatacg ctgaaaattg cgtggaatgc
    38881 atccgatgag caggggcttt cttacttttc cgcactgcgt gacagcggga aaaagctgat
    38941 ctgcattgat ccaatgcgat cggaaaccgt cgatttcttt ggcgataaaa tggagtgggt
    39001 ggcaccgcac atgggcaccg atgttgcgct gatgctgggg atcgcccata cgctggtgga
    39061 aaatggttgg cacgacgaag cgtttctgac gcgttgcacc acaggttatg acgtcttcgc
    39121 ctcttatttg ctgggcgaga gtgacggaat agcgaaaacc gccgagtggg cggcagagat
    39181 ttgtggggtt aacgcagaga aaatccgcga actggcggct attttccacc aaaacaccac
    39241 catgctgatg gctggctggg gaatgcagcg ccaacagttt ggtgaacaaa aacactggat
    39301 gatcgtcacg ctggcggcaa tgttggggca aatcggcaca cccggcggcg gttttggtct
    39361 ttcttaccat tttgccaatg gtggtaaccc cacgcgccgc gctgcggtgc tctcttccat
    39421 gcagggcagc ttgccgggtg gctgcgatgc ggtagataaa atccctgttg cccgcattgt
    39481 tgaagcactg gaaaaccctg gtggcgcata tcaacacaac ggtatggacc gacatttccc
    39541 ggatattcgt tttatctggt gggcgggcgg tgccaacttt actcatcatc aggataccaa
    39601 tcgcctgatc cgtgcctggc aaaaaccgga gctggtggtg atctctgaat gcttctggac
    39661 ggctgcggcg aaacacgcgg atatcgttct gcctgcgacc acttcgtttg agcgtattga
    39721 tctcaccatg accggcgatt acagtaatca gcatctggtg ccgatgaagc aagtggtgcc
    39781 tccacgttat gaagcgcgta atgattttga tgtctttgcc gagttgagtg aacgttggga
    39841 gaagggcggc tatgcgcggt ttacggaagg aaaaagtgag ctgcaatggc tggaaacgtt
    39901 ttataacgtt gcccggcagc gcggggcaag ccagcaggtt gaattgccgc catttgctga
    39961 gttctgggaa gccaaccagt taattgagat gccggaaaac ccggacagtg agcggtttat
    40021 tcgcttcgcc gattttcgcc gcgatccgca ggcgcatcca ttaaaaaccg ccagcggtaa
    40081 gattgaaatc ttctcgcagc gtattgccga ttatggttac ccggattgcc ctgggcatcc
    40141 gatgtggctg gagccggatg agtggcaggg caatgccgaa ccagaacagt tgcaggtact
    40201 ttctgcccat ccggcgcacc gcctgcacag ccaactgaat tacagctctc tgcgcgaatt
    40261 atacgcggtg gcaaatcgtg agcctgtcac cattcatcct gacgatgccc aggcgcgcgg
    40321 catacaagat ggcgatattg ttcggttgtg gaacgcacgc gggcaaattc ttgccggagc
    40381 ggtcattagc gagggaatta aacctggcgt gatttgtatt catgaagggg catggccgga
    40441 tctggattta accgctgacg gtatttgtaa aaacggcgcg gtgaacgttc tgaccaaaga
    40501 tctccccagc tcgcggctgg ggaatggctg tgcgggtaat acggcgctgg catggctgga
    40561 aaaatacaac ggtccggaac tgacacttac agcgtttgaa ccaccggcca gctcataatc
    40621 caggtgggta gttgggtttc atcctgccat gcgcagtcga caatgtgaaa accctgcgca
    40681 tggtagaaat ctatcgccgg ttgatttttt tgataaacct ccagcaacag atggggatgg
    40741 cgctgctgca catactgcat tagcgcctta ccaataccgc gtctgacggc cttcggtgcg
    40801 acaaacattg ctgcgaggaa tcggccttcc ataatgctga caaaaccgag aagcttaccg
    40861 tcttcttccc agacccagtt ttgcgcgttg gcaagatagg catcccgcac cagcggaatg
    40921 caatcacgcc agtaattcgc ttttataaag ggatgccccc aggttgtact ttccagccac
    40981 agttcgagga tcgcggggag ttctgaacgt tgcgcttccc gaatcatggt ttatttcccg
    41041 gatagcaaca gcagccaacc acatgatcat tcaccagccc acatgcctgc ataaaggagt
    41101 aacagattgt ggtaccaaca aacttaaaac cacgcttttt cagggcttta gatagagcgt
    41161 cggaggcggg cgtagatgta gggatttcgc tcaacgttgt ggcttgtgtc acctgtggtt
    41221 gatgattgac aaacgaccag acaaagtcgg caaacggttc gccgttctgt tccatttgca
    41281 ggtacgcccg cgcattacca ataattgcct gaattttccc gcgatggcgg ataatcccgg
    41341 cgtcctgtac cagtctttcg acatcctctt cctgcattgc agccaccttc accggatcga
    41401 actgatgaaa gctggcgcga tagttttcgc gttttttgag gacggtgatc cacgataatc
    41461 cagcctgctg cccttcaagg cagatcattt cgaacagttt tttactgtca gtttcaggca
    41521 cgccccactc attatcatgg taggcaatat aaagcgggtc ctgactcacc cagccgcaac
    41581 gttccatact ttccctcgct atgtacccga tcgtaaaaat ttcagccgct tttcttgacg
    41641 catcggtgaa aaagtcaata tttaatacag ggctactatg cccgatatcc ttcatacaat
    41701 gacaataata ttgccgaatt cggctcttcc ctggagtcgt gttaatgatc ataaaaaaaa
    41761 gcggtggtcg ctggcagcta agcctgctgg cgagtgtggt aatcagtgcc ttttttctca
    41821 acacagctta cgcctggcaa caagaatata tcgttgatac gcaacccgga cattccacag
    41881 agcgttacac ctgggatagc gatcatcaac ctgattacaa cgatattttg tcgcaacgta
    41941 ttcaaagtag ccaaagggcg ctgggactgg aagtcaatct ggcggaagaa actcctgtgg
    42001 atgtgaccag cagtatgagt atgggctgga attttccttt atatgagcag gttacaaccg
    42061 gcccggtcgc ggcattacat tacgatggca caaccacctc gatgtataac gagtttggcg
    42121 acagtactac cacgctggcc gatccgttat ggcatgccag cgtgagtacc ttaggctggc
    42181 gtgttgactc ccggcttggc gatctccgac cctgggcgca aatcagctat aaccagcaat
    42241 ttggcgagaa tatctggaag gcgcaatcag gcctgagccg gatgacggcg acaaaccaga
    42301 acggcaactg gctggatgtc accgtaggcg ctgatatgtt gctcaatcaa aatattgccg
    42361 cctatgcggc gctatctcag gcagaaaata ccactaacaa tagcgactat ctgtatacga
    42421 tgggggttag tgccagattt taacgtaaca gtcacaatta aaaccattga gtaacaatag
    42481 ttgtggtgat agtgggtgct aacttactaa ataataaatt tggtgaataa ttgtcgagtc
    42541 attcattcct gaactaatgc atttcattcc gttctgatgg catttcatgc cgtttttccc
    42601 caggcataaa gtgcacttcg ttatggttgt cagcagagat ttttcctttt tattactgca
    42661 ggaatactgc catgacacct tcaaattatc agcgtacccg ctggttgaca ctcatcggta
    42721 ctatcattac ccagtttgcg ctggggtcgg tttatacctg gagcctgttt aacggcgcgc
    42781 tttctgccaa gctggatgcg ccggtaagcc aggtcgcttt ctctttcggc ttgttaagtc
    42841 tggggctggc aatttcatct tctgttgcgg ggaaattaca ggaacgtttt ggcgttaaac
    42901 gcgtcactat ggcttccggc attttgctgg gattaggctt cttcctgaca gcgcattctg
    42961 acaacctgat gatgctgtgg ttaagcgccg gtgtgctggt cggtctggcg gatggcgcgg
    43021 gttacctgct gacgctctct aactgcgtga agtggttccc ggagcgtaaa ggtctgatct
    43081 ccgcgttcgc tatcggttct tatggtctgg gtagcctggg tttcaaattt atcgacacgc
    43141 agctgctcga aacggtgggt ctggaaaaaa cttttgtgat ttggggagcg attgcgctgg
    43201 tgatgattgt tttcggcgca acgttaatga aagacgcacc aaaacaggaa gtgaaaacca
    43261 gcaatggtgt cgtggagaaa gattacacgc tggcagagtc gatgcgtaaa ccgcagtact
    43321 ggatgttagc ggtaatgttc ctgactgcct gcatgagcgg cctgtacgtg attggggtag
    43381 cgaaagatat cgcccaaagt ctggcacacc ttgatgtggt ttccgcagcc aatgcagtca
    43441 ctgttatttc catcgccaac ctttcaggtc gtctggttct ggggatactg tctgacaaaa
    43501 tcgcccgaat ccgtgttatc accattggtc aggtgatttc gctggtgggt atggcggccc
    43561 tgctgtttgc accattgaat gcagtgacgt tctttgcagc gattgcctgc gtggcgttta
    43621 actttggcgg cactatcacg gtgttcccgt cactggtcag tgagttcttc ggcctcaata
    43681 acctggcgaa aaactacggt gtgatttatc tcggtttcgg tatcggcagc atttgtgggt
    43741 cgattatcgc ctcactgttt ggcggcttct atgtgacctt ctacgtgatt ttcgccctgc
    43801 tgattctgtc actggcgctc tctacgacga ttcgtcagcc agagcagaaa atgttgcgtg
    43861 aggcgcatgg ctccctttaa tcatctgccg cgagctactg ggctttagcc ttagtgtgaa
    43921 tcaaacgctt caacgcaatg tataaacaat gacgcgtaaa atttgggcca gttaactggc
    43981 cctttttact gctttttgta acgctaaagt ctcttttcaa acttgcattt ttgtaaattt
    44041 gtgcttcgtg cacactcttt ccccacactt tttccctttg ctgtggtcta cttattcgcg
    44101 cgtgtagatt ttacttatct gactacctcc gcacttttcc cctgcctggc ctgaaaagcc
    44161 attaagcagg gtgttatcac ctgtttgtcc agggtttgtt tgcatgagat acatcaaatc
    44221 gattacacag cagaagctga gctttttgct tgcaatctat attggccttt ttatgaatgg
    44281 cgcggttttt taccgccgct tcggcagcta tgcgcacgat tttaccgtct ggaaaggcat
    44341 ttctgctgtt gttgaactgg ccgccaccgt actggtgacc ttctttttac tacgtcttct
    44401 ttcgctgttt ggccgccgta gctggcgtat tctggcgtcg ctggtggtgc tcttttccgc
    44461 aggtgccagc tattacatga ccttccttaa tgtggtcatt ggttatggca tcatcgcttc
    44521 cgtcatgacc accgatatcg acctgtcaaa agaagttgtt ggtctgaact ttattctctg
    44581 gttaatcgcc gtaagtgcat tgcctcttat ccttatctgg aataaccgct gtcgttacac
    44641 cttgctccga cagctgcgaa cccctggaca gcgtattcgc agcctggcgg tcgtcgtgct
    44701 agctggtatt atggtttggg caccgattcg tttgctggat atccagcaga agaaagtgga
    44761 gagggcgacc ggtgttgatt tgccaagcta tggcggcgtc gtagcgaact cttatctgcc
    44821 atcaaactgg ctttcggcat tggggctgta tgcctgggcg cgggtcgatg aatcttccga
    44881 taacaattca ttgcttaatc cggcgaagaa attcacttat caggcaccgc aaaacgttga
    44941 tgacacttat gtcgtgttta tcatcggtga aactacgcgt tgggaccata tgggcatttt
    45001 cggctatgag cgtaatacca cgccgaagct ggcccaggag aaaaatctgg cggcgttccg
    45061 tggctattct tgtgacacgg cgaccaaact ttctctgcgt tgcatgtttg tacgagaggg
    45121 tggagcggaa gataatccgc agcgcacatt aaaagaacag aacattttcg cggtgctgaa
    45181 gcagttagga ttcagttctg acctctacgc tatgcagagc gaaatgtggt tctacagcaa
    45241 cacgatggcg gacaacattg cttatcgtga gcagattggt gcggagccac gtaaccgtgg
    45301 caagccggta gatgatatgt tgctggtaga cgaaatgcag caatcgctgg ggcgcaaccc
    45361 ggatggcaag catctgatca ttttgcatac caaaggttcg cactttaact acacccagcg
    45421 ttacccgcgc agctttgcgc agtggaagcc ggaatgtatt ggtgttgata gcggctgtac
    45481 caaagcgcag atgatcaact cctatgacaa ctcggtgacc tatgtggatc actttatctc
    45541 cagcgtgatt gatcaggttc gcgataagaa agcgattgtg ttctacgcag ctgaccacgg
    45601 tgagtcaatt aacgaacgcg agcacctgca cggcacgccg cgtgaactgg caccgccgga
    45661 acagttccgt gtgccgatga tggtctggat gtcggataaa tatctggaaa atccggtcaa
    45721 tgcgcaggca tttgcgcagc tgaaaaaaga agccgacatg aaagtgccac gccgtcacgt
    45781 agagctgtac gacaccatta tggggtgtct tggctatact tcacctgatg gtggaattaa
    45841 cgaaaacaac aactggtgtc acatcccgca ggcaaaagag gcagcggcta actaagcggc
    45901 ctgctgactt tctcgccgat caaaaggcat tttgctatta agggattgac gagggcgtat
    45961 ctgcgcagta agatgcgccc cgcattcggt gattggcgca gcctggtagc gcacttcgtt
    46021 cgggacgaag gggtcggagg ttcgaatcct ctatcaccga ccaaattcga aaagcctgct
    46081 cagcgagcag gcttttttgc atttgttgac ccgtcctgaa tagcgttgac acgttcctga
    46141 cttaaatccg gagaacgtga tgatgactga gttcaaacgc acccaacgtg attatcctct
    46201 atcctttaaa atagctgttg ttgagcaggt tgaaaaaggc gagatgacct ataaacaggc
    46261 ccagcagcgg tatggcattc aggggcgctc caccgttctt gtctggttac gtaaatatgg
    46321 ccggcttgac tggagtcctg gacttcctga cctggtgaag aggaaactgc ctgtgactca
    46381 gacaactatc ccgctcacac ccgagcaaag aatcagggaa cttgaagcgc agcttgagct
    46441 ggcgaaccag aaagctgagt tttttgagtc agttatcaat gtcctta
//
LOCUS       DAPHCV010000024        45845 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.024, whole genome shotgun sequence.
ACCESSION   DAPHCV010000024 DAPHCV010000000
VERSION     DAPHCV010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.024"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            215..841
                     /locus_tag="RH033_001372"
     CDS             215..841
                     /locus_tag="RH033_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HDV2990584.1"
                     /translation="MTPVDRPLLNIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNLNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            862..2964
                     /locus_tag="RH033_001373"
     CDS             862..2964
                     /locus_tag="RH033_001373"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HDV2990585.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLNIKDDKVSLEKADLLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEDMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRKTLQR
                     LGLEDIAADLAAHNLLPA"
     gene            2977..3615
                     /locus_tag="RH033_001374"
     CDS             2977..3615
                     /locus_tag="RH033_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HDV2990586.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLENIKQNGDHADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDIAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3679..4347
                     /locus_tag="RH033_001375"
     CDS             3679..4347
                     /locus_tag="RH033_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDV2990587.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTMQEKKVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4344..5129
                     /locus_tag="RH033_001376"
     CDS             4344..5129
                     /locus_tag="RH033_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HDV2990588.1"
                     /translation="MNPSQHAEQFQSQLANYVPLFTPQFWPVWLIIAGLLLVGMWLVL
                     GLHALLRARGVKKSANDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     TVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5133..5945
                     /locus_tag="RH033_001377"
     CDS             5133..5945
                     /locus_tag="RH033_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2990589.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERAFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGEDSTIVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(5951..7561)
                     /locus_tag="RH033_001378"
     CDS             complement(5951..7561)
                     /locus_tag="RH033_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HDV2990590.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EVGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEEIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAIAQAVVKPASRARRRLSFNQM"
     gene            complement(7687..7992)
                     /locus_tag="RH033_001379"
     CDS             complement(7687..7992)
                     /locus_tag="RH033_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HDV2990591.1"
                     /translation="MATLLQLHFAFNGPFGDAMVEQLEPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8100..8176)
                     /locus_tag="RH033_001380"
     tRNA            complement(8100..8176)
                     /locus_tag="RH033_001380"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8140..8142),aa:Val,seq:gac)
     gene            complement(8181..8257)
                     /locus_tag="RH033_001381"
     tRNA            complement(8181..8257)
                     /locus_tag="RH033_001381"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8221..8223),aa:Val,seq:gac)
     gene            8566..9822
                     /locus_tag="RH033_001382"
     CDS             8566..9822
                     /locus_tag="RH033_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HDV2990592.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDKGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKANDTLLAGGTMNNLGGEDSDTIVENGAIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSVGNINL
                     NGGKLWLITDAATQVHLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9863..11236)
                     /gene="mdtK"
                     /locus_tag="RH033_001383"
     CDS             complement(9863..11236)
                     /gene="mdtK"
                     /locus_tag="RH033_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HDV2990593.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRAAR"
     gene            11451..12092
                     /gene="ribE"
                     /locus_tag="RH033_001384"
     CDS             11451..12092
                     /gene="ribE"
                     /locus_tag="RH033_001384"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDV2990594.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLEGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12132..13280)
                     /gene="cfa"
                     /locus_tag="RH033_001385"
     CDS             complement(12132..13280)
                     /gene="cfa"
                     /locus_tag="RH033_001385"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HDV2990595.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13571..14782)
                     /locus_tag="RH033_001386"
     CDS             complement(13571..14782)
                     /locus_tag="RH033_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HDV2990596.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCQNH
                     GNAEVAHSESH"
     gene            14895..15827
                     /locus_tag="RH033_001387"
     CDS             14895..15827
                     /locus_tag="RH033_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2990597.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15824..16849)
                     /gene="purR"
                     /locus_tag="RH033_001388"
     CDS             complement(15824..16849)
                     /gene="purR"
                     /locus_tag="RH033_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HDV2990598.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17148..17237
                     /gene="ynhF"
                     /locus_tag="RH033_001389"
     CDS             17148..17237
                     /gene="ynhF"
                     /locus_tag="RH033_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HDV2990599.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17403..18572
                     /locus_tag="RH033_001390"
     CDS             17403..18572
                     /locus_tag="RH033_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2990600.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPEAVCVANS"
     gene            complement(18718..19299)
                     /gene="sodB"
                     /locus_tag="RH033_001391"
     CDS             complement(18718..19299)
                     /gene="sodB"
                     /locus_tag="RH033_001391"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HDV2990601.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19427..20254)
                     /gene="mepH"
                     /locus_tag="RH033_001392"
     CDS             complement(19427..20254)
                     /gene="mepH"
                     /locus_tag="RH033_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HDV2990602.1"
                     /translation="MARINLISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASANFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20589..20936
                     /gene="grxD"
                     /locus_tag="RH033_001393"
     CDS             20589..20936
                     /gene="grxD"
                     /locus_tag="RH033_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HDV2990603.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(20987..25603)
                     /locus_tag="RH033_001394"
     CDS             complement(20987..25603)
                     /locus_tag="RH033_001394"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDV2990604.1"
                     /translation="MADNLNPSSPLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDQLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLQGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSMERLDTLLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTTRLNELYAARLQRSPSIAVDAAHFE
                     STSGATSNRVQSSDIFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     PPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFAASLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLHELGPMTVEDLAQRHTGSSEEIASYLE
                     NLLAVKRIFPTMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTSEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMQVIEQL
                     AGVGLPASLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     PAESFTPAEANQANRSALQQAIVTVLADGGAWFAQQISQRIRDKIGESVDPSAMQEAL
                     WALVWQGVITSDIWAPLRALTRSSTNTRTSTRRSHRARRGRPVYAQPVSPLVSYNTPK
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANAWGNL
                     LPWPAHPATLIPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEIFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(25696..26343)
                     /gene="rnt"
                     /locus_tag="RH033_001395"
     CDS             complement(25696..26343)
                     /gene="rnt"
                     /locus_tag="RH033_001395"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HDV2990605.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHNFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(26446..26853)
                     /gene="gloA"
                     /locus_tag="RH033_001396"
     CDS             complement(26446..26853)
                     /gene="gloA"
                     /locus_tag="RH033_001396"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDV2990606.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(26934..28031)
                     /gene="nemA"
                     /locus_tag="RH033_001397"
     CDS             complement(26934..28031)
                     /gene="nemA"
                     /locus_tag="RH033_001397"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HDV2990607.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLKV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(28068..28667)
                     /gene="nemR"
                     /locus_tag="RH033_001398"
     CDS             complement(28068..28667)
                     /gene="nemR"
                     /locus_tag="RH033_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HDV2990608.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRDSHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            28770..29009
                     /locus_tag="RH033_001399"
     CDS             28770..29009
                     /locus_tag="RH033_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HDV2990609.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            29058..29954
                     /locus_tag="RH033_001400"
     CDS             29058..29954
                     /locus_tag="RH033_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HDV2990610.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            30035..30556
                     /gene="sodC"
                     /locus_tag="RH033_001401"
     CDS             30035..30556
                     /gene="sodC"
                     /locus_tag="RH033_001401"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HDV2990611.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEVKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(30557..32569)
                     /locus_tag="RH033_001402"
     CDS             complement(30557..32569)
                     /locus_tag="RH033_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDV2990612.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWRPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPDATREIL
                     EQLLTALASSQTDVYTVARIISPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDRARLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDCLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(32569..33426)
                     /locus_tag="RH033_001403"
     CDS             complement(32569..33426)
                     /locus_tag="RH033_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDV2990613.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNFYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQAQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHASSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFTLDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(33429..33665)
                     /locus_tag="RH033_001404"
     CDS             complement(33429..33665)
                     /locus_tag="RH033_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HDV2990614.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVIHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            33866..34300
                     /gene="slyA"
                     /locus_tag="RH033_001405"
     CDS             33866..34300
                     /gene="slyA"
                     /locus_tag="RH033_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HDV2990615.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLIKLIAKLEHNIIELQAKG"
     gene            complement(34347..34814)
                     /gene="slyB"
                     /locus_tag="RH033_001406"
     CDS             complement(34347..34814)
                     /gene="slyB"
                     /locus_tag="RH033_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HDV2990616.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRQVQIQGGDDSNVIGAIGGAVLGGFLGNTIGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTHFSPGQRVVLASNGSQVTVSPR"
     gene            35088..36197
                     /gene="anmK"
                     /locus_tag="RH033_001407"
     CDS             35088..36197
                     /gene="anmK"
                     /locus_tag="RH033_001407"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDV2990617.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGGRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            36295..36624
                     /gene="mliC"
                     /locus_tag="RH033_001408"
     CDS             36295..36624
                     /gene="mliC"
                     /locus_tag="RH033_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HDV2990618.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLFERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            36683..37339
                     /gene="pdxH"
                     /locus_tag="RH033_001409"
     CDS             36683..37339
                     /gene="pdxH"
                     /locus_tag="RH033_001409"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HDV2990619.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPAEPLTLFERWLSQACD
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            37468..38742
                     /gene="tyrS"
                     /locus_tag="RH033_001410"
     CDS             37468..38742
                     /gene="tyrS"
                     /locus_tag="RH033_001410"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDV2990620.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            38801..39664
                     /gene="pdxY"
                     /locus_tag="RH033_001411"
     CDS             38801..39664
                     /gene="pdxY"
                     /locus_tag="RH033_001411"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HDV2990621.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(39708..40313)
                     /gene="gstA"
                     /locus_tag="RH033_001412"
     CDS             complement(39708..40313)
                     /gene="gstA"
                     /locus_tag="RH033_001412"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HDV2990622.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKSTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(40419..41921)
                     /gene="dtpA"
                     /locus_tag="RH033_001413"
     CDS             complement(40419..41921)
                     /gene="dtpA"
                     /locus_tag="RH033_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HDV2990623.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(42532..43167)
                     /gene="nth"
                     /locus_tag="RH033_001414"
     CDS             complement(42532..43167)
                     /gene="nth"
                     /locus_tag="RH033_001414"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDV2990624.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPPAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(43167..43862)
                     /gene="rsxE"
                     /locus_tag="RH033_001415"
     CDS             complement(43167..43862)
                     /gene="rsxE"
                     /locus_tag="RH033_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HDV2990625.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(43866..44486)
                     /gene="rsxG"
                     /locus_tag="RH033_001416"
     CDS             complement(43866..44486)
                     /gene="rsxG"
                     /locus_tag="RH033_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HDV2990626.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLETTAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDVHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(44490..45548)
                     /gene="rsxD"
                     /locus_tag="RH033_001417"
     CDS             complement(44490..45548)
                     /gene="rsxD"
                     /locus_tag="RH033_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HDV2990627.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHGIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(45549..>45845)
                     /locus_tag="RH033_001418"
     CDS             complement(45549..>45845)
                     /locus_tag="RH033_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="part of membrane-bound complex thought to be
                     involved in electron transport to nitrogen; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDV2990628.1"
                     /translation="EPEEQVDPRKAAVEAAIARAKARKLEQQQANAVPEEQVDPRKVA
                     VEAAIARAKARKLEQQQANAEPEEQVDPRKAAVAAAIARVQAKKAAQQKVVNED"
BASE COUNT        11511 a        11323 c        11975 g        11036 t
ORIGIN      
        1 tgataacgat caatgttaag agcccagaat aatcacatta ttttttctcc taaccataaa
       61 ggattagttt atcagcgtta aatcttttct catacactat cgggttaggg aaaatatctg
      121 gtgttgagga cagcgtcagc agcgtattag ttgagtgcct aatatcaggc tgctgaattg
      181 tcagcactgt gtttgcgaag aggatgcgaa ataaatgacc ccggttgatc gtccactatt
      241 aaatattggc ttaacgcgat tagaattctt gcgtatatca ggaaaaggcc ttgctggttt
      301 aacaattgcc cctgctttac tgtcactttt gggttgcaaa caggaagata ttgatagcgg
      361 cacagtaggg ttgataaata cacccaaagg ggtgctggtg acccaacgtg cgcgatgcac
      421 gggttgccac cgctgtgaaa tctcttgtac caacttgaac gatggctcag tagggacatt
      481 cttctcccgt atcaaaatcc atcgcaatta tttctttggc gacaacgggg ttggctctgg
      541 cggtggcctg tatggcgatc tcaactatac cgcggatacc tgccgtcaat gcaaagagcc
      601 gcaatgcatg aacgtctgcc cgattggtgc gattacctgg cagcagaaag aaggctgtat
      661 taccgtcgat cataaacgtt gtattggctg tagcgcctgt actacggcat gcccgtggat
      721 gatggccacc gtaaataccg aaagtaaaaa atcctcgaaa tgtgtgttat gcggtgaatg
      781 cgcaaacgcc tgcccaacag gggcgttaaa aattatcgag tggaaagata ttactgtgtg
      841 aatcttgcaa aggaaaaaat catggctaac ggttggacag gtaatatatt aagagtcaat
      901 ctcacgacag gaaatattac cctcgaagat tccagtaagt ttaaaagttt tgtcggtggc
      961 atgggcttcg gctacaaaat tatgtatgac gaagtaccgc caggcacgaa acctttcgat
     1021 gaagcgaata aattagtctt tgctaccggc ccattaactg gatctggtgc cccctgtagt
     1081 tctcgcgtaa atatcacctc actttctact tttaccaaag gaaatttagt cgtcgatgcc
     1141 catatggggg gcttttttgc agcgcaaatg aaattcgctg gatacgacgt cattattatc
     1201 gaagggaaag cgaaatcacc ggtatggctg aatattaaag atgacaaagt tagcctggaa
     1261 aaagccgatt tattatgggg aaaagggacg cgcgcaacga cggaagagat ttgtcgattg
     1321 accagccctg aaacctgtgt ggcggctatt ggtcaggctg gggaaaacct tgttcctctc
     1381 tctggcatgt tgaatagccg taaccacagc ggcggtgcgg gaactggcgc aataatgggt
     1441 tcgaaaaacc tgaaagcgat tgcggttgaa gggacgaaag gggtcaacat tgccgatcgt
     1501 caggagatga agcgtctcaa cgattacatg atgactgaac ttatcggtgc gaataacaac
     1561 catgtcgtgc caagtacgcc gcaatcgtgg gcagagtatt cagatcccaa gtcacgttgg
     1621 acagcacgta aagggctgtt ttggggcgcg gctgaaggtg gtccgattga aacgggggaa
     1681 attccgccag gcaatcagaa cacggtcggc tttcgtacct ataaatccgt ttttgactta
     1741 ggtccggcgg cagagaaata cacagtaaaa atgagtggct gccactcttg cccgatccgt
     1801 tgtatgaccc aaatgaatat tcctcgggtg aaagagtttg gcgtgcccag cacaggtggt
     1861 aacacttgtg tagcaaactt tgtccatacc accatcttcc cgaacgggcc gaaagatttt
     1921 gaagataaag acgatggtcg tgtgattggt aacctggttg gtctgaattt gttcgatgac
     1981 tacggcctat ggtgtaacta cgggcagttg catcgcgact ttacttattg ttacagcaaa
     2041 ggtgtgttca agcgtgttct gcctgctgaa gagtatgcgg aaattcgctg ggatcaactg
     2101 gaagcgggtg acgttaactt tattaaagat ttttactacc gtctggcgca tcgcgtgggt
     2161 gagttgagtc acctggctga tggttcatat gccatcgcag aacgctggaa tttgggtgaa
     2221 gagtactggg gctacgcgaa aaataaactc tggtcgccgt ttggctatcc ggttcaccat
     2281 gccaatgaag cgtcagcgca agtcggttcc attgttaact gtatgttcaa ccgtgactgc
     2341 atgacgcata cccatatcaa ttttattggt tccggcttgc cattgaaact gcaacgtgaa
     2401 gtggcgaaag aactttttgg ttctgaagat gcttacgatg aaaccaaaaa ctacacgcca
     2461 atcaatgacg caaaaattaa atatgctaag tggtcgctgt tgcgggtctg tttgcataac
     2521 gccgtcaccc tgtgcaactg ggtctggcca atgaccgttt cgccgctgaa aagccgtaat
     2581 tatcggggcg atctggcgct tgaagccaaa ttcttcaaag cgatcactgg cgaagatatg
     2641 actcaggaaa aattagattt agctgcagag cgtatcttta cgttgcatcg cgcctacacg
     2701 gtaaaactga tgcaaactaa agatatgcgt aatgaacacg atcttatctg ttcctgggta
     2761 ttcgacaagg atccgcagat cccggtcttt actgaaggta cagacaaaat ggaccgtgac
     2821 gatatgcatg cttcgctcac gatgttctac aaagaaatgg gctgggaccc acagcttggt
     2881 tgtccgaccc gcaaaacatt gcagcgtctg gggctggaag atattgccgc cgatctggca
     2941 gcacacaatc tactgcctgc gtaaggaatg ggtgagatga accatcagga tgaattgccg
     3001 ttagcgaaag tcagcgaagt tgatgaggcg aagcggcaat ggttgcaggg gatgcgtcac
     3061 cccgttgata cggtaaccga accagaacct gctgaaatcc tggcagagtt cattcgtcag
     3121 cattctgcgg ctgggcagct ggtggcgcgc gccgtgtttc tctcgccgcc ctattcggtt
     3181 gcagaagagg aactgtcagt tttgctggaa aacattaagc agaacggaga ccacgcggat
     3241 atcgcgtgta tgacgggaag ccaggatgat tactattact caacgcaggc catgagtgaa
     3301 aactacgcgg cgatgtcttt gcaagttgtg gagcaggaca tttgtcgcgc cattgcacac
     3361 gccgttcgtt ttgagtgcca gacgtatcct cgtccttaca aagtcgccat gctgatgcaa
     3421 gcgccatatt acttccagga agcgcagatc gaagccgcga tcgccgcgat ggatattgca
     3481 cctgaatatg ctgatatacg gcaggtggag tcatcgacgg cggtgttgta cttgtttagc
     3541 gagcgattta tgacctatgg caaggcgtat ggcttatgcg aatggttcga ggtagagcag
     3601 tttcagaacc cctgaaattt cagggagatg tcatggatag ggtggacagt agcagccact
     3661 gcattagggg ataatcagat gtccttcact cgacgcaaat ttgttctggg gatgggaaca
     3721 gtaatctttt ttactggttc tgcgtcttct ctgttagcga acacgatgca agaaaagaaa
     3781 gttcggtatg ccatgattca tgacgagtca cggtgcaatg gctgtaatat ttgcgcccgc
     3841 gcatgccgta aaactaacca cgtccccgct cagggaagcc gtttatcgat agcacacatt
     3901 ccagtcaccg ataacgacaa cgaaacgcag tatcactttt ttcgccagtc atgccagcac
     3961 tgtgaagatg ctccttgcat cgacgtttgc ccgacagggg cgtcgtggcg tgatgagcag
     4021 gggatcgtgc gggtagaaaa atcgcagtgc atcggctgca gttattgcat tggcgcttgt
     4081 ccttaccagg ttcgctacct taaccctgtc actaaagtgg cagataaatg tgatttctgc
     4141 gctgagtccc gactggcaaa aggctttccg cccatttgcg tcagcgcctg tccggaacac
     4201 gcattgatct ttggtcgcga agatagccca gaaattcagg cgtggttgca ggaaaataaa
     4261 tactatcaat atcagctacc tggtgccggg aaaccgcacc tgtatcgccg gttcggtcaa
     4321 catttgatta aaaaggaaaa tgtatgaacc cgtcgcaaca tgctgaacag tttcagagcc
     4381 agttggcgaa ctatgtgcca ctgttcactc cccaattttg gccggtgtgg ttgatcattg
     4441 ccggactatt gctggtaggg atgtggttgg tgctggggct gcatgccttg cttcgtgctc
     4501 gtggcgtgaa gaaatcagcc aacgaccatg gtgagaaggt ttatctttac agcaaagcgg
     4561 tcagattatg gcactggtcg aatgcgttac tctttgtatt gttgctggca agtgggctga
     4621 taaatcactt tgcgctggtg ggcgcaacta cggttaaaag tctggttgcg gtgcatgaag
     4681 tttgcggatt tttgttactg gcatgctggc tcggctttgt tctgatcaat gccgttgggg
     4741 gtaatggtca ccactatcgc attcgtcgtc aggggtggct ggagcgagcg gcaaaacaaa
     4801 cgcgattcta tttgtttggc attatgcagg gggaagaaca tcctttcccg gcaacaaccc
     4861 agtctaaatt taatccctta cagcaggttg cctatgttgg tgtcatgtat ggattgctgc
     4921 cgttgttact attgacgggg ctgctgtgtc tctatccgca agccgtggga gatgtgtttc
     4981 ctggcgtaag atactggtta ttgcaggcgc attttgctct ggcatttata agcctctttt
     5041 ttatcttcgg tcatctttat ctttgcacca cggggcgtac gccacacgaa acctttaaaa
     5101 gcatggtcga tggctatcac cggcactaac gaatgatcat tacacgagca gacctgcgag
     5161 aatggcgtat cggagcggtg atgtatcgct ggtttctgcg ccatttccca cgcggcggta
     5221 gttatgccga tattcatcat gcactgattg aggaaggata tacggactgg gcggagagtc
     5281 tggttgagta tgcgtggaaa aaatggctgg cggatgaaaa cttcgcgcat caggaagttt
     5341 cttctatgca aaaactggcc acagatcccg gagagagagc attttgcagt cagtttgccc
     5401 gcagcgacga tcatgctcgc atcggctgtt gtgaggataa tgccagaatc gcaaccgctg
     5461 ggtatgccgc gcaaattgcc agtatgggtt atagcgtgcg gattggaagt gtcggcttta
     5521 acagtcacat tggcagctcc ggtgaacgcg cacgggttgc tgtaaccggt aattcttcgc
     5581 ggataagcag cgctggagat agcagtcgga ttgcgaatac cggaatgcga gtacgcgttt
     5641 gtacattggg tgaacgatgt catgtcgcca gtaatggtga cctggtgcaa atcgccagct
     5701 ttggcgcaaa cgccagaatt gccaacagcg gcgacaatgt acacatcatt gccagcggtg
     5761 aagattcaac aatagtgagc acgggcgtgg tcgactcaat tattctcggg ccagggggaa
     5821 gcgccgcact ggcatatcat gacggcgaaa gagttcgctt cgccgtcgcc attgaaggtg
     5881 aaaacaatat tcgagccgga gtccgctatc gccttaatga acagcatcag tttgttgagt
     5941 gctgatagct ttacatttga ttaaacgaaa gacgccgacg agcacgggat gcaggcttta
     6001 cgaccgcctg agctatcgcc tcaccaattt ctgcacatgc cgtaagtccc tgtacgaatg
     6061 gctggtcgtg catcaaccag ggcaacgcgc cgaacgctac gcgcccacga atttcttcgg
     6121 gttgctgtag cgtataatct tcgccaacat cagggatttc atcccctgtt ttctgtaatt
     6181 gttcgcgtag tccagggaaa ggaatatctt tcactttaag cggacgctgc ccacgggcat
     6241 caataaaaac gtcaaacgag tagctattgt cttccgtttt caggacggtg cgcgactcat
     6301 taatttccat ttcgtagtct tcaccgaggg cgagaatatg aatgattccc gcttcacgta
     6361 aggccagtag tcgacgaata gactctgaag ggattgccgc ataattatcg atgaaaaccc
     6421 gggcaaggcc tttactgaac cgtttatggt cgtgttcatt cagatgagga acaatttcct
     6481 gtacggcttc atgcaggcgc agaatgacat aacgccaggg aacagtatgt ttttctcgtt
     6541 tattgcgttc gacttcctgg agattttttt ctgcccagtc gaatgggtcg cgttgtttgc
     6601 gctcggcaaa ccaggcttga gcaaaggaat cgacattcag gctttctaag gctatgcgtt
     6661 ggctccagtc tggatcagca aatttgattt cctctactat caatctaaat acccgatcca
     6721 aaaggcccac ttcgcctttt tgaatctcag catttaatgc ttgatcggtg acgatgtgta
     6781 agggctcgta gggaataggg caatagaaat cggcttcggg taaaataccc gtacgcgaca
     6841 tcaacgtaat atttagcttt tcacttgcgt tatcgcggtg aaaaacgacg tgttgtttat
     6901 catcttcaat gaacgaacca tgctgaatag ccactgccat tgccgcatcc agtccgctca
     6961 aggatgttcc cataataccc acgttacacg catcgacctt tgcttccatc aggcctgacc
     7021 acgggctggg aaaatacgtt cgggttgctt cttcttcatc aggccagacg tgacccgtgg
     7081 cgatcaccgc taaatcaaac gtctcgctgg gtaaatcctg atttgtagcg agcatgacgc
     7141 cagcatttgt aatttgcaga tcggtaacct ggcatgattc ataaacagcc actgcaaatt
     7201 tttgctgtcg tgcctggtct actaatcgta aaaattgatc gcggaaatat tcgcccagca
     7261 gaattcgcgg taaaaactga cgatcgtgca aggtttcttt tttaacgcca taacgctgga
     7321 gatggctggc ttcttgcttt tgtagccatt cgagatacgt acaataaatc ggcggtattt
     7381 caatactggc aatatttgcc agcatcattt ttgagttttc ctcatcactg tatggcatcc
     7441 cgacaccggc ttcatcagcc tgctcgaaga tagaaataga aagtggagtt tgttgctgta
     7501 gaagcgagaa taaggtgtag atccccgtag gcccggcacc cacaatagca atttttttca
     7561 ttgttactta cctgtacgca ttctttcgaa tttagtgtta tcagataagt ttggttgaga
     7621 tttttagaat ttgagaaatt tcagacaggg atgaaaaggg cgctaaccgc ccttgattaa
     7681 attctgtcag gcgagttttg cctgattgat ttgactaagt ggctcattga catcgaaaac
     7741 tttggcgaca acttcctcaa cgccgagatt tttcagtcgg gcagtatgtt tttccagata
     7801 ggcaagggcg cttttttcat cagtaaaaag gtagatccca ccggcttcgt ggttcttttc
     7861 actttctgtc cataccttcc acagaaaacc aggttcctga ttaatcgact cagcaagcgg
     7921 ttcaagctgc tcaaccattg cgtcgccaaa tgggccatta aaagcaaaat gaagttgtaa
     7981 caaagttgcc atagcgattc tccttctgat aacgcatacg aagacagtgt aaatctttta
     8041 acgtattgga tacagagaag aaacagaggg aaaaaggaga agaccagatt aagaaaatct
     8101 ggtgcgtccg agtggactcg aaccaccgac ccccaccatg tcaaggtggt gctctaacca
     8161 actgagctac ggacgcagga tggtgcgttc aattggactc gaaccaacga cccccaccat
     8221 gtcaaggtgg tgctctaacc aactgagcta tgaacgcaac gttgtaggtg acaacgggga
     8281 cgaatattag cggcagagtg ggaaggtggc aagaggcaaa acgtaatttt ctgcgctatt
     8341 tcgaccgttt gcagagcttt taagcaaatt ggctatattt tgttgatttg caagggcgat
     8401 ttttattcag gatcgcattt acatcagata caacccaata atccgtgtat cagagtttaa
     8461 acagagttgt ttgccaccat ctcaaaatct gtcgtggata aatcctccgt agcctttaca
     8521 cttaggtagc aataaaggca cacagatgca gcaaaggaat aaatcatggg atctgatgcg
     8581 aaaaacttga tgagcgacgg gaacgtgcaa attgttaaga ccggcgaggt cattggcgcg
     8641 acgcaactta ctgaaggtga gttaattgtt gaagctggcg gaagagccga aaataccgtg
     8701 gtcacggggg ctggctggtt gaaagtggca accggtggga tcgccaaatg cacacagtac
     8761 ggcaacaatg gcacgctatc ggtcagcgac ggtgccattg ccacagatat tgttcagtcc
     8821 gagggaggcg caattagtct ctctacgctc gctacggtta atggccgcca tcccgaaggt
     8881 gaattcagcg ttgataaagg ttatgcctgc ggtttgttgc tggaaaatgg cggtaacctg
     8941 cgtgtactgg aaggacatcg cgcggaaaaa attattctcg atcaagaggg tggcctgttg
     9001 gtcaatggga caacctcagc ggtcgtggta gatgaaggtg gtgaattgtt ggtgtatcca
     9061 ggtggggaag ccagcaattg tgagattaat cagggcggcg tttttatgct ggcggggaaa
     9121 gccaatgata cgttgcttgc tggtggcacc atgaataatc tcggtggtga agactctgac
     9181 actattgttg agaatggagc catctatcgt ctgggtacgg atggtcttca gctctacagt
     9241 tccggtaaga cgcaaaacct gtccgttaat gtgggggggc gggctgaagt gcatgccggt
     9301 acgctggaaa atgcggtaat acaaggtggg acagtgatcc tgttgtcacc caccagcgcg
     9361 gacgaaaatt ttgtagtaga ggaagatcgc gcaccggttg aactgaccgg tagtgttgca
     9421 ttactggacg gcgcttcaat gattattggc tatggcgcag atctgcaaca atcaacgatt
     9481 actgtacagc agggcggtgt gttgattctc gacggcagta cggtaaaagg tgacagtgtc
     9541 actttcagtg ttggtaacat caatctcaat ggcggaaaac tgtggctgat cactgatgcg
     9601 gcaacgcagg tgcatctgaa agtgaaacgc ctgcgtggag agggagccat ttgcctgcaa
     9661 accagtgcga aagaaatttc acctgacttc atcaatgtga aaggggaagt tactggtgat
     9721 atacacgttg agataacaga tgccagtcgg caaactctgt gtaacgcact gaaactacag
     9781 ccagacgaag acgggattgg cgcaacgctc cagcctgcgt aaaaacacag gcgcaacccg
     9841 tgaaggttgc gccttgtctt tactagcgag ctgctcgttg cagaatgatg gctgacggca
     9901 gacgttgcag gaaccgcata cgcaacatca tcataatggc tgccgacgtc aggccaataa
     9961 taaagcctat ccagaagcct gctggcccca taggttcaac gaccagatcg gtcagtgcca
    10021 gaatatagcc gcttggcaag cccagcaccc agtaagccgt aaaggtgata tagaaaatgg
    10081 aacgcgtatc tttataacca cgcaaaatcc cactgccaat cacctggata gagtcagaaa
    10141 tctgatatac cgccgccagc aacatcaaat gcgcagccag cgttacaacc tcgggattgt
    10201 cgttgtacaa cagggcgatt tgctcccgca gtgaaaccgt gaaaatggcc gtcagggttg
    10261 ccatacagac acccaccata agcccggtcc gcgcagcggt ttgcgcatcc agcgttgagc
    10321 cctgacccag acgataacct acgcggatag ttaccgctgc cgccagcgac atgggaagca
    10381 caaacatcag cgaactaaag ttcagggcaa tctggtgtcc tgcgacatca acaataccga
    10441 gcggagacac taacagagcc acgacggcaa acagtgtcac ttcaaagaac agtgccagcg
    10501 caatcggcaa accgagttga atcagtcgtt tcataaccgc aggatcgggc ttagcggtgc
    10561 ctttttcgtt acgaatatcg cgcatggagc gggcgcgttt aatgtaagaa accatggcaa
    10621 ggaacatgac ccaatacacc gccgcagtag ccacgccaca accaacgcca ccgagctcag
    10681 gcataccgaa atgaccataa ataaagatat agttcaccgg aatgttcacc agcaggccga
    10741 taaagcccat taccataccc ggcttggttt ttgccagacc ttcacattgg ttacgggcaa
    10801 cctggaagaa cagatagccc ggcgcgcccc acaacaacgc acgcagataa cccacggctt
    10861 tgtccgccag agccggatcg atgttttcca tagagcggat aatgtaacct gcattccaca
    10921 gcaccagcat aatgagaacg gaaacaaaac ctgccagcca gaagccctgt cgcacctgat
    10981 gcgcaatgcg ctcacgtcga ccggaaccat ttaattgcgc gataaccggc gttaatgcca
    11041 gcagcagtcc gtgaccaaag aggatcgccg gaagccagat agaagtaccg atagcgaccg
    11101 ccgccatgtc ggtggcacta tagccgcccg ccatcacggt atcgacaaaa cccatcgcag
    11161 tttgggcgat ttgcgcgaga atcaccggga ttgctaatgc taataacaga cgcgcttcac
    11221 tgatatactt ctgcacgtga acacctttta tttgtagtta tatgagagac taaaaagccg
    11281 cctgaatggg cagcaaaaag aagaagcagg ggaaattcag tctattgtag cgaggattta
    11341 ttaattctcc agtgaaaaaa tagtcattct taccccttca ctggtaaggg cgtttgccac
    11401 ctgctattgt gctgaacaga atgtcttaac tgatttcagg agttgtaagt atgtttacgg
    11461 ggattgtaca gggcaccgca aaactggtgt cgattgacga gaaaccaaat tttcgtacgc
    11521 atgtggtgga gttacccgac cacatgctgg agggcctgga aaccggtgcg tccgtggcgc
    11581 ataacggttg ctgcctgacc gtgacggaaa ttaacggcaa ccatgtcagt tttgacctga
    11641 tgaaagaaac gttacgcatt accaatcttg gcgatttaaa agtgggggat tgggtaaacg
    11701 ttgagcgtgc ggcgaaattc agtgatgaaa ttggcggaca cttaatgtca ggtcatatta
    11761 tgaccaccgc tgaagtggcg aaaatattaa cctcggaaaa taatcgccag atctggttta
    11821 aagtccagga tagtcagttg atgaaatata ttctgtacaa aggatttatt ggcatcgacg
    11881 gtattagcct gaccgtcggc gaagtcacgc caacgcgttt ttgcgtccat ttaattccgg
    11941 aaaccctgga acgcacgact cttgggaaga aaaaactcgg cgcacgcgtc aacattgaaa
    12001 ttgatccaca aactcaggca gtggtagata ccgtagaacg tgtgctggca gcacgagaaa
    12061 atgccatgaa tcaaccaggt acagaagcct gatctgaaat cgcccccgga gagggggcga
    12121 tagaatagcc tttagcgagc gactcgaagg ccgttttcaa caccgcgtga gaacaccacc
    12181 tgccagagct gaatatcacg ggcgcggaaa gcacctgcac aggcattcag ataataggta
    12241 aacatccgtt taaagcgttc actgtagtta tccgcaattt ctggccatgc ggcgaggaat
    12301 cgttcatacc acgccatcaa cgtagtatcg taatcagcac cgaagttatg ccagtcttcc
    12361 atcacaaagt ggggttcgct ggactgagca atctggcgta cagagggcag gcaaccgttc
    12421 ggaaaaatat atttattaat ccagggatca acattcagat cggttttttt cgaaccgata
    12481 gtatggagca ggaatatgcc ttccggtttc aaattacgat ccaccaccgc aaaataggta
    12541 tcgtaatttt tcggtccgac gtgctcgaac atccccacag aaacaatacg atcaaactgg
    12601 tcgttcaggt cacgataatc ttgcagcaaa atggtgacat ccaggccttc acagcgttcc
    12661 tgagccattt tttgctgttc ggcagaaatg gtgacgccaa ccacgcttac gtcataatta
    12721 gatgccatgt agtgcgccag tccgccccag ccgcagccaa tatccagtac gcgcatccct
    12781 ggttttaact gcaatttttc acaaatcatt ttgagcttcg cctgctgggc agattccaga
    12841 ttatcggcat ctttccagta agcgcaggaa tattgcatga agggatcaag catgcggctg
    12901 aacaagtcat tacccaaatc gtaatgctct ttgccgacta tccaggcacg ttttttactc
    12961 tgcagattga agagacgagc gccggcaata cgcagcgtgt ctttgaaatg atgggggagt
    13021 tggttctcga gacctgcgcg taagactttg ctaaaaaaca tatccagtcg gtcacattcc
    13081 caccagccat ccatataact ttcgcctaac cccaacgatc cttcctgcag aacgcgttta
    13141 aaaaaatcgg ggtttttcac acgaatatcc gccggggcag aaccgttaat ggctataccg
    13201 gcacggctaa gtaattcgtt ggcgatacgg taccagttgt catccggtac actgacttct
    13261 tctatacacg atgaactcat agtttctcca tcactggtcg gtgatcagaa ccttaaaaca
    13321 gcgtagacgc tttttgggct ttgtgagaaa tctcgcagaa aaaaccgcga gtctataatc
    13381 cgacgtagaa cagagggaaa ggaggaaccc ttgccgaaat gaccatccat agtgagtcgg
    13441 gaacgatcct gtccccgtaa aaacataata tttatcgtag ctttaacaac tcgaaattag
    13501 tataggccgc taatttcaat catacaacaa aattttgtta gcagcctaag tataagtata
    13561 tcgatataga tcagtgtgat tcgctatgag cgacttcggc attgccatga ttctggcagt
    13621 caacttcttc acaacgttgc atcatgtaac ccagagcaac cagcactact gttgataaca
    13681 tcacgctggt ggtggtgagc aatggcgtgc tgatactgat cagccaggaa actaccagac
    13741 ttgcgaggaa gcacagaccc agttgtagag tgttctgcaa cgctgcggcg cgaccggttg
    13801 catgtgggaa gggacgcagc gcctgggcga caacaatcgg gtagatcgcg ccattggcaa
    13861 tcgccatcac acagaatggg atcaggattt cgactagcga cacatggcta atgaagcccg
    13921 cagcccaggt cgcaatgacg ctgacagcaa acagtaccag caaccacggt aataactgct
    13981 tgccttgcca tttctgcagt gcggcgcgac agccataacc accaatcaga aacgcgatag
    14041 tttgcgggac ataacttaaa ccaataactg ccgggctgta gcccatttca ctaaggatga
    14101 acggtgagcc ggtcagccat gcaaaaaaac tggctgaaca ggccgcatat atcagcacgt
    14161 tgccgcgata ggttttagaa cgtagcaggt cggtaaaggt cagaccatcc tgactattgt
    14221 tacgggcctt cgtcgtgggt ttgagccaga aaataggcag aatcagcacc acggtaatgg
    14281 caaacagggt ggcgaaaatc gcctgccagg aaaaatggac cagcagccag cttcctaaca
    14341 gaggagccag tgccggagat agacccacca gcggcatgat ggtcgcaaaa atacggttaa
    14401 ctttctgtga aggataataa tccgtcacta acgcttgcca gataactgcc gcggcgcaga
    14461 cacccacagc ctgtacaaaa cgcaatacca gcagcgtagc ggcgttttct acccacagca
    14521 tccccagact acctaacgca aaaattgtca ggccgattaa taataccggt ttacgaccat
    14581 aacggtcgga gagcggcccc cacagaagct gggctgcggc aaaaccggca aggaacagac
    14641 taaggctggc actgacagca gacgcaggcg tttgcaggtc ggcctgtatg gcggcgaaag
    14701 caggcagata catatcggtt gccagaaaac cgagtacgct caaacccgcc agccagacta
    14761 aaaatctttt cccaggttgc atgtatattc tctaaatctc tcaattaaac accgccgcaa
    14821 agtgtaggga gtgaaattag tgttgtgaaa cggtaatatt tgatgactgg tttcaaaaat
    14881 tttgcaggca gactatgtgg tcagaatatt cactcgaggt tgttgatgcg gtagcgcgta
    14941 atggtagttt tagcgctgcg gcacaggagt tgcatcgcgt tccttctgcg gtcagctata
    15001 ccgtgcgtca gctggaagag tggctggcgg tgccgctgtt tgaacgacgt caccgtgatg
    15061 tggaactgac cgctgccggg gcgtggtttc tcaaagaagg gcgctctgtt gtcaaaaaaa
    15121 tgcagatcac ccggcagcaa tgtcagcaga tagcgaacgg ctggcgcggt cagttagcta
    15181 tcgcagtgga taatattgtc aggccagaac gtacacggca gatgatcgtt gatttttatc
    15241 gccattttga tgatgtcgaa cttcttgtct ttcaggaagt gttcaacggt gtctgggatg
    15301 cgctttccga cgggcgcgtg gaactggcga ttggcgcaac acgcgcgatt ccggtaggcg
    15361 gtcgttatgc cttccgcgat atggggatgc taagctggag ttgcgttgtt gccagccacc
    15421 acccgctggc gttgatggat ggcccgttca gcgatgatac gttgcgcaac tggccgtcgt
    15481 tggtgcgcga agacacctcg cgaacgctac ctaaacgtat tacctggttg ctggataacc
    15541 aaaaaagact tgtcgtgcct gactgggaat catcggcaac ctgtatctcg gcaggattat
    15601 gcatagggat ggtgccaaca catttcgcca aaccgtggct taatgagggg aagtgggtag
    15661 cactagaact ggaaaacccc ttcccggatt cggcatgttg tctgacatgg cagcaaaatg
    15721 atatgtcgcc tgcgttaacc tggttgcttg agtatctcgg cgatagtgag acgctgaata
    15781 aggagtggct gcgggagccg gaagagactc ccgcaacagg tgattaacga cgatagtcgc
    15841 ggaacgggcc gtcagccacg gagcggcgtt caatcaaccg cggatgcact tcaatggact
    15901 gcggttcttc acgtttgttg acgatacgat ccaacagcat gttgaacgct gtttcgccca
    15961 gcgaatcttt tggctgatgg atcgtggtca gcgccggcgt aaaatagcgg gcgttgcgca
    16021 cgttatcata accgatcagc gaaacatcct gcgggacgcg cagacccatt tcatcagcag
    16081 cacaaagtgc gcccattgcc atgatatcgc caccacagaa gacggcagta gggcgatgcg
    16141 gctgcgacag gatttgctgc atggcgcgat aaccggattc aggttcaaag tcaccctgca
    16201 caatccagct ttccggcact ttgatcatcg cttcttccat cgccttcata aaaccggcaa
    16261 ggcggcctgc gccggtgtta cgttccagcg ggccagggat aacgccgatt tcgcgatgac
    16321 cgcgttcaat cagataacgc ccggccatgt agccgccttc gaatgcgtta tcaatgaccg
    16381 catcggtgaa gtcagctttt gcttcacccc agtccatcac caccattggg atatggcgat
    16441 actcttccag catcgccagc aacggctctg ggtactcaga acacatcacc agcagaccat
    16501 cgacgcgttt ttgcgccatc atcgacagat aagcccgctg tttctcaaga ttgttccacg
    16561 cattgcccag aatcagggtg taacctttct ggaagcaatt tttttcaacc gcttcaatga
    16621 tctcggcaaa ataggccgct tcgctgctgg tcgccagcaa accgatagac ttggtgtggt
    16681 taaccttcag gctacgcgcc accgcgctag gggagtagtg taattcttta atcgctgccc
    16741 acacggcgtt gcgcgtttct tcagcgacga aacgtgtttt gttgatcacg tgtgacacag
    16801 ttgtagtgga aacgtttgct cgtttcgcta catcttttat tgttgccatt ccatttcact
    16861 ccagacccta acttcagctc ctgcaaaatc gcaaggtaaa cgtttgcctt tacacacctt
    16921 acgcacaaca aaagtgttgc ggtacgccgg aaaaacgaca cggagcgtca ggaaggggtc
    16981 aatggccagt acgcaaatag tgtaagcgtg gaattttggc cgatcttgac gaaaagggga
    17041 agtggtaaaa acacttatca ttataaatga cgggagattt tttcatcaca gtgtgtaaaa
    17101 atgcgtaata tcactttttg tgtggtaata aaaaaggaga aaacttgatg agcaccgacc
    17161 ttaaattttc actggtaaca acgattatcg tcctcggttt gatcgtggcc gtgggtttga
    17221 cagccgcgct gcactgattt cctctcgagg gagcgcgttc tccctcgcat ctccttcctg
    17281 tttgttagca aaatggcaaa agtgttttct ctttttgtta ttttcatttt tttgtgattg
    17341 ctgtcatgct ttcgccactt taatgaatat cgcgtaatga cgcgaagagg tggagataag
    17401 gcatgaaaat taactatccg ttgctggcgc tggcgattgg cgcgtttggt atcgggacaa
    17461 cggagttctc gccaatgggc ttattgcccg tcattgcgcg cggtgtggat gtctcgattc
    17521 ccgctgccgg aatgttaatc agtgcctatg cagttggcgt aatggttggc gcgccgctga
    17581 tgacgcttct actttctcat cgtgctcgcc gcagtgcgtt gattttcctg atggcgattt
    17641 tcacgctcgg caacgtactt tccgccatcg cgccggatta tatgacgctg atgctttcac
    17701 gcattttgac cagcctgaat cacggagcat tttttggttt gggttcagtt gtggccgcaa
    17761 gcgtggtgcc aaaacataaa caggccagcg cagttgccac tatgtttatg gggttaaccc
    17821 tggcaaatat cggtggcgtt ccggcagcga cctggttggg cgaaaccatc ggctggcgga
    17881 tgtcatttct ggcaacggcg gggctgggcg tgatttcaat ggtaagtctg ttcttctcat
    17941 tacctaaagg tggcgcaggg gcgcgacctg aagtgaaaaa agagctggcg gtattaatgc
    18001 gtccgcaggt gctgtctgca ttgctgacga cggtactggg agctggtgca atgtttaccc
    18061 tctacaccta tatctctccg gtactgcaaa gtattaccca cgcaacgccg gtgttcgtta
    18121 cggcaatgct ggtgctgatt ggtgtcggat tctctatcgg taactatctc ggcggcaaac
    18181 tggcagatcg ttcagttaac ggcacgttga aaggcttttt gttgctgctg atggtgatta
    18241 tgctagcaat cccgttcctg gcccgcaatg agttcggcgc agctattagc atggtggtgt
    18301 ggggagctgc aacctttgcg gtcgtaccgc cgttacagat gcgcgtgatg cgtgtcgcca
    18361 gtgaagcgcc tggtctgtct tcatcagtca atattggtgc ctttaatctt ggaaatgcgc
    18421 tgggagctgc tgctggtggt gcggtaattt ccgctgggct gggatacagc tttgtgccgg
    18481 tgatgggggc gattgtcgcg ggactggcat tattgctggt gtttatgtca gccagaaaac
    18541 aacctgaggc agtttgcgtt gccaacagct aattacacaa aaacgcagaa ggataacctt
    18601 ctgcgttaca gttgctggca acgtgcgatt tatcttgccg gatgcggtgt taacgcttca
    18661 tccgagttgg catggtaagc gaagtgcatc aggcaatgtt gcatttgcca tcagttatta
    18721 tgcagcgaga tttttcgcta cgaattccca gttcaccagc gcccagaagt gctccagata
    18781 gccaggacgt gcattgcgat agtcgatgta ataagcgtgt tcccagacat caacggtcag
    18841 cagcggagtc gcatcggtgg tcagcggagt acccgcgtta gaggttgaaa cgatagccag
    18901 tttgccatcg ctgtttttca ccagccaggt ccagccagaa ccaaagtttt tgattgctgc
    18961 atcagtaaac tgtgctttga aatcggcaaa gctgccaaaa gatgcggcga tagcttcagc
    19021 gacttttcca gtcggttcgc cacccgcgtt cggtgccagg cagttccagt agaaagtatg
    19081 gttccagacc tgagctgcgt tgttgaatac gccaccttca gagctgcgaa taatctcttc
    19141 cagtgattta ccttcaaacg cggtaccttt aatcaggttg ttcaggttag tgacataagt
    19201 ctgatgatgc ttgccatagt gatactcgat ggtttccgca gaaatgtgcg gtgccagagc
    19261 atctttagca tatggtagtg caggtaattc gaatgacatt gctactctcc tttattatta
    19321 atttgcatac gtacaatagc cttattgtgc gtatggatag ggtagcaaat taaaaggtga
    19381 gacaaaagat gaacttaccc tgttgccgta acaacagggt aaagttttag cgaagtgttt
    19441 ttggggtcat tacccgacga gcgccaacat agtggcgctg ccagtagtct tcactgagag
    19501 aagtgatttg aatttcttgg cccgtgcgcg gtgactgaat aaatttgccg ttgccgacat
    19561 acacgccgac atgatcggct gtaccgcgtc cctgagtacg gaagaagacc aggtcgccgt
    19621 ttttcagttc actacgttcg ataggtgctg catcacgcag gtgatacatt tcattcgccg
    19681 tacgcggaat acgaattttc accaaatctt tataagcgta ataaaccagg ccgctgcaat
    19741 caaaaccggt acgcggtgag ctgccgcccc aacgatatgg cttaccaatt tgttgcatca
    19801 gtttattcat tgccactttc gtggcttttt gcaccttcgc tttgtgcgcg tcggcaaggg
    19861 ttcctgacgc ggcatttttg actttcacac aatgcgattt atagccctta cgtttggtac
    19921 acttttcggt aaagttggct gaggccgttt tattgacggc atgtttgctg cgagttttgg
    19981 cgtttttaga agcggtaatg gaagattttt taacggaaga agcggttttg cttttggtcg
    20041 tacttttact ggcggctttt ttcgctgttt tctggctggc tttctgggtc tttttggtgg
    20101 ttgccgtgct tttcttttta tctgcttttt tggtgatatg agtagtagca gaactctccc
    20161 tggcttgctt tgaagcatgg gccataggcg ttaaaggcag ggtggtaaaa agtaaagcac
    20221 agagcgtgat cgaaattagg tttatccgcg ccactgagcg tccctctgtt aaggcaggtc
    20281 tatcatgcct gcgtctgatt tacaaaactt gatgattcta atgcatagaa gaagagaaag
    20341 ttaatagctt ttattgttaa aaaatacgtt tcgttggcga gcgcaaaaaa atgcagcagt
    20401 gaaagctaaa aatggttata aatacgccag tttgccgtgc gtcagggaag aaacgaatat
    20461 ttgaaatgca acaaatagag caaagggata aaatagcaaa agcgtgatga aaatgttatt
    20521 ttccgatgcc gcactgaccc gctacaatgt cagattactg ccgtaaagaa gtttgaggaa
    20581 gcaagacaat gagcaccact atcgaaaaaa tccaacgcca gattgctgaa aacccgatcc
    20641 tgctgtacat gaaaggttca ccgaaactgc cgagctgcgg tttctctgcc caggcagtcc
    20701 aggcgcttgc cgcatgtggc gaacgttttg cctatgttga tatcctgcag aatccggaca
    20761 ttcgtgcgga actgccgaaa tatgctaact ggccgacctt cccgcaactg tgggttgacg
    20821 gcgagctggt cggcggttgt gatatcgtga tcgaaatgta tcagcgtggc gaactgcagc
    20881 agctgatcaa agaaactgcc gctaaataca agtctgaaga gccggacgct gaataatccg
    20941 tcctgcaata ctgacggatg cgaacgggca tccgtcagtc ctttctctat ccccaatcca
    21001 gcccttgtgg cgaacttgaa aatcccgcct cacgcagcag cgtaaacatc ggcgtttgcc
    21061 ggaccggtag gtcattcact tctgttagcg taaagcgcag ccgtggttcg cgacgcagtg
    21121 cggtagtcag cgcgtggaaa atctctgggg cgagtaattc ctctttttcc tgccagacca
    21181 gcattttttt gccaccttgc gccagataga gtaacaattt gccgccagaa acgaccacca
    21241 gcgcacccgc ccgacgcgtt ggtatcagcg ttgccggatg tgcaggccag ggcagaagat
    21301 ttccccatgc attagccgga tcgttggcag agagcgccac tggtgtatag tggcgcgttt
    21361 gcgtggcttg tgtcgccaga tcgcgcaatc ggtcaatagt cagacgttca gcgaattgcg
    21421 cgccacccag accttctaca aaacgacctc gcataattcg cccggagtct tccatacttc
    21481 gacaaagcgt ttgcatcgat ggaaacccgc cagggatatt ttcggctatc accgcctgac
    21541 gactgatgat gccgtagcgg tcgagcatat tttccgccag cgccagcatc ctttcggtat
    21601 cgtttagtgg ctccacctgc aataacgacc agcgtccagc aagctttggt gtgttgtaag
    21661 ataccagcgg cgagacgggt tgcgcataga caggacgtcc acgacgagcc cggtgactgc
    21721 ggcgagttga ggtgcgtgtg ttggtactgc tgcgggtgag ggcgcgtaac ggtgcccaaa
    21781 tgtcgctggt gatgacgcct tgccagacca gcgcccataa cgcctcttgc atggctgagg
    21841 gatcaaccga ttcgccgatt ttatcgcgta tccgttggct gatttgttgt gcaaaccagg
    21901 ctcctccgtc agccagaacc gtgactatcg cttgctgcag cgccgaacga ttcgcctgat
    21961 tcgcttcggc gggagtgaac gattctgcgg gatattcctg tagatgcagt gccaccagac
    22021 cgtcatcttc acccagcttt ttttgccccg accagataac cgcaccggtt gccagtaatt
    22081 catcgagcat ttctggcgaa tagtcgcgta cgcgggcagg caggatctgg ctttcccaga
    22141 gtgaggcggg taaaccgact ccggcaagct gttcgatcac ctgcatgacg ccatccacgc
    22201 cctcataaac gcctggcgat gttgaggcaa agagtgccgg gctaccatcg gtggcgggta
    22261 atacgccctg acgttccagc agcaatcgcg cataggtcgt ggccgcaacg ggacgcgtcg
    22321 cttctctggc ggcttgcagc gagcgcaaac gcagacgacg aaatacttca tcgctcaccc
    22381 agatgtcttg ttgcagattc atcaccagcc cctgttcgcg cagttgctga agttgctctt
    22441 cgacaatggc aataccgaga ctaaactcat gagccagttg ttcagacgtg accagagcat
    22501 gagcccggag atagcgcaga aagaggtcgc gaagcgggta actgactcgg tgcaaataaa
    22561 tctctggcag tgactctggt agtcgtacgc cgagggcatc gcgcagcctg gcggcatcat
    22621 ccatacaggc aagacgctcc tgcccgctaa tcatcgtcgg gaagattcgt tttactgcaa
    22681 gaagattttc cagatacgac gcaatctctt cactgctgcc tgtatgccgt tgcgccaggt
    22741 cttcaacggt cattggcccc agttcgtgca gcaggtcgaa caatccttct tcacctttcg
    22801 ctcttctgcc aggagccagt cgttgcaact cttcttccac ctggcgaatg acctgcgggt
    22861 cgagtaattc ccccggatcg acctgtccga gtagattgcg cagtaactcg ctgtccagcg
    22921 acagtacgga tgcccggcgc tctgccagcg gggcgtcgct ctggtacata aattccgcga
    22981 catagccgaa caataaactg gcggcgaaag gcgaaggcgt agtggtcgtt acatcggata
    23041 tttgaatttc gccaccgttc aggcgacgca tcaaacgttc cagcgcggga agatcataaa
    23101 catcttgcag acattcgcgt agggtttcga gaatgaccgg aaaatctgga tatccctgag
    23161 cgatttccag caactgactg gcgcgcagcc gttgttgcca gagcgggggg cgatggcccg
    23221 gcgtgcggcc cggcattaat aatgcccgcg cggcgcattc gcgaaaacgg gcggcgaaaa
    23281 gtgccgagct gcctaccgcc tcgcggacaa tttgcagcaa cttttctggt tcaaacaaaa
    23341 aaatcgcggc atcgggcaat ttaccatcgg tgtcaggaat gcgcgcaaca atgccgtcat
    23401 cactggcaac cacagacgcg tcagcgcccc atagtgcatg tattcgcccg gcaatcgcca
    23461 gcgcccaggg ttcatgcacc cgtcttccat agggagagtg caaaataata cgccagtcac
    23521 caatttcatc acggcaccgt tcgaggacaa gatgacgact acctggaacg atgcccgtcg
    23581 cgttgcgctg ctcgtcaatc aacccctgaa tattggcgat cgtattttct tctgccaacc
    23641 acgggggaat agtcccggaa aagaacgcgc catccgccag taaatggaga aaatcgccga
    23701 tcatctcgcc taattctgcc ggacgtccgt taccttcacc acgccagaag gggagccggg
    23761 cagaacgacc cggagcagga gtcacaatca cctgatcgcg ggtgatttgc tggatccgcc
    23821 atgaggtagc gccgagagtg ataatgtcgt tcacccgcga ctcatatacc atctcctcat
    23881 ccagttcacc cacccgccgc gaaccggcct gttcttcacc ttcgggtaat aacacgctat
    23941 acatgccacg atccggaatg gtgccgccgc tggtaaccgc caacaattga gcgccaggtc
    24001 gggcggtcaa tatcccggtc tcccggttcc agaccagttt ggggcgaaaa gcagaaaaat
    24061 cgccagaggg atagcgcccg gaaagcatat ccagcgtggc gtcaaaaaca cgtcttggca
    24121 gatctttcca cggtgcggca cggcgtacgc gggagtacca ttcgtctacc tgtaatgcat
    24181 ccatcgccgc ggcggcaacg gtttgctgcg caaggacgtc gagtggatta tgcggcggtg
    24241 tcaggttttc cagcctgcct gcgaacatac actctacaat gactgcggaa tcgactaagt
    24301 cacgcctggt tcgggggaaa aaaagccctt tagatacacc gccaacctga tgtccggcgc
    24361 gaccaatgcg ttgtaaccca ctggcaacag aaagcggcgt tgccacctga atcaccagat
    24421 ccaccgcgcc catatcaatc cccagttcga gactggaggt tgcgaccaca cagcgtaatt
    24481 cacccgattt caacgcctgt tcggtgattg ctcgttgttc tttagagacg gagccgtggt
    24541 gtgagcgggc aataaaaatg tcgctacttt gtacacggtt agaggttgcg ccggaggtcg
    24601 actcgaaatg ggccgcatca acggcgatag atggggaacg ctgtaagcgt gcggcgtaaa
    24661 gctcatttaa tcgtgtcgtc agtttttccg ccagcccgcg tgaattagta aagacaatgg
    24721 tcgagcgatg gcgcaaaact tcatcaagga tacccgtttc aatatatggc cagatggagc
    24781 cttcccggcc ggcatggctg tcttcgccgg tgccgctggc gaccgatgag acatcatcca
    24841 tattggcgac cggtacgaca atccgtatct gcggatggcg cattgcgggc gggttgacta
    24901 ccgtaaccgg gcgatcgcca ccaagaaatg ctgccacatc gctggctgaa cgcacagtgg
    24961 cagaaaggcc aattcgctgt gctgaggtgt ggagcagcgt atcgagccgc tccatactta
    25021 acgccagatg cgcaccacgt ttactgcccg ctaccgcgtg gacttcatca ataattaccg
    25081 tttcgacgcc gcgtagcgtt tcgcgcgcgc gggaggtcag catcagatag agtgattcgg
    25141 gtgtggtgat cagaatatcc ggcggattac gggtgagttt gctgcgttcc tgtgcaggcg
    25201 tatcgccagt acggatcccc acgcgaagat tgacttccgt ttcgccgcgc cgccgccgtt
    25261 catcggcaat accctgtaac gggatctgca agttgcgctg aacgtcggtg cccagagctt
    25321 tgatcggtga aatatagagg atgcgtgagg ttttacgctt atgcgcctcg cgggtatctt
    25381 cgccgccttc gcggaagagc tgatcgaggg cgtagagaaa tgctgccagc gttttcccgg
    25441 agccagtcgg tgcaatcacc agcgcatgtt cgcctcgcgc cgccacatgc caggtttgcg
    25501 actggacagc ggtcggctgt ttaaaggcgc gaagaaacca gtcgcgggtc gccggtgaaa
    25561 acacgtccgg caggggcgat gaagggttta gattatctgc cattaacgct attccccgtg
    25621 aatatcatgc atgatgcgtg gaatagcctc actatagatg cctgaatcat tgcatgtcat
    25681 caggcatcga ctcgattaca cctcttcggc ggcaggtagc ggccagcctc ccagacgttt
    25741 ccagcggttg acgatttcac aaaacagcac agcagtgcgt tcggtgtcgt acagcgcgga
    25801 gtgcgcctgg gtgctgtcga agtccatgcc agcggtctgg caagcctttg acaatacggt
    25861 ttgtccgagt gccagcccgg ccagtgcagc agtgtcaaaa gtggcgaaag ggtggaacgg
    25921 gttacgtttc agtgaggcgc gttctgcggc ggccatcata aagttgtgat cgaaattggc
    25981 gttgtgcgcc accataatgg cgcggttaca gccgctcgct ttaatgcctt tacgtacaac
    26041 tttaaaaatt tcgtgcagcg cctcgtattc gctgaccgcg ccgcgatcgg gatcgttcgg
    26101 gtcaatgccg ttgaaggcga gggcctctgg ctgcaaattt gcgccgacaa atggttcgac
    26161 gtggaaatgt aatgtggtgt ccggcatcag ccagccttgt tcatccattt tcagggtgat
    26221 ggcggcaatc tcaagcagcg catcggtttt ggcgttaaat ccggctgttt caacatcgat
    26281 cacaacagga taaaaaccac gaaaacggtc gcacagaccg gtaagttgag cgttatcgga
    26341 catctgattc tcttaatacg gataaattgc agcgcgcatt atgacaaata ttacttgtcg
    26401 atgcagtaaa gatgcgggcg cgatgagttc acgcccggca ggagattagt tgcccagacc
    26461 gcgaccggcg tctttctctt cgattaactc aattttgtaa ccgtccggat cttccacaaa
    26521 cgcgataacc gtagtaccgc cttttaccgg acccgcttca cgggtcacgt tacccccgtt
    26581 ttgacggatt ttttcgcacg cttcagcggc gttatctacg ctaagcgcga tgtgaccata
    26641 agcagtgccg agttcgtatt tatccacgcc ccagttgtag gtcagttcaa tcaccgcttc
    26701 ttcggtttcc ggaccgtagc caacaaacgc cagtgagtat ttgtattccg ggttttcgct
    26761 ggtacgcagc agtttcatgc ccagcacttt ggtataaaaa tcgatggagc gttgcagatc
    26821 gccaacgcgc agcatggtat gaagaagacg catcttttta tcctcaaaat ggctaacaga
    26881 ttcaaaatgt tgttttagta tagcggcggc tttgcgccgc tcgcaatgtt ggattacagc
    26941 gtcgggtaat cagtgtagcc ttccgcgccg ccaccgtaaa aactttcggc acgctgtggg
    27001 ttaagctcag ctttgcgttg caagcgggcg accagatccg ggttcgcaat ccagtcacga
    27061 ccaaatgcca ccgcatcaat taaccctttg ccgatcagcg tttcggcttt ttctaccgtg
    27121 tatgcacctg cgccgataat cggaccgtgg aaacgggcgc gtactttttc gcggaacgca
    27181 tcagtatacg gttcaccccc cgcccaatct ggttctgaca tatgcagata agcaatgccg
    27241 cgtttaccca gttgttctat cagatacagt gcatcggctt cttcattcgg gccgttatct
    27301 gtgttctgga aagtaccgat tggcgaaacg cgaatgccaa tgcgatcggc accccattct
    27361 tcaatcccgg catcgaccac tttcagcacc agacgtgcgc gattttccac gctgccgccg
    27421 tactgatcgg tacgatggtt tgaagaagga gaaaggaact gatgcagcaa ataaccatga
    27481 gcagagtgga gctctaccag atcaaaaccg gcttcacgcg cgttagcaat ggcctgacgg
    27541 aaatcattga cgatacccgg aatctcttcc agttcaagcg cacgcggcat ggatgtttca
    27601 acacggatcg cctgaccatt ttcatcgcgc agagaagtac gtgttcccgc gctaagtgcg
    27661 gaaggcgcta ccggtgcctg accgccaggt tgcaggctgg cgtgagaaat gcgtccggtg
    27721 tgccacagct gtacggccat atgaccattt tcagcatgaa cgccagcggt aatttttttc
    27781 catgcggcaa tttgttccgg actatggatg ccaggtgcac cagcataccc ttttgcctgg
    27841 gcagaaattt gcgtggcttc gctaataatc aaaccggcac tggcacgttg gcgatagtat
    27901 tccgccatca acggggtagg gatgtcaccc ggttcaatac tgcgcagacg cgtcagcggt
    27961 gccataaaaa tacggtttgc cgccgtgatc gcgcccactt tcagtgggga atacagtttt
    28021 tcagatgaca taaaggctcc cggtcgtctg gtgataaaaa atgcctgcta aacggcaggc
    28081 gtcgcaataa tggtttttac atgtgccagc gcgttttcca gtggctcgga attgcgcgaa
    28141 attttggcct gcagattcgc acccagccat agtgcgtaaa gcacttgtgc ctgttgcagc
    28201 ggttcgccac aaaaggttaa acaatggcta tcacggccat tttccagcgc ctgcgagagc
    28261 agggcgatca cgccgcgagc gcctttatcc atcgcgctac gcatatcttc tgacagatcg
    28321 cacacttcgg cagagagttt tactgtcagg caaccactga tggttccatg ttggcaaaac
    28381 tggttcagtg tttgctggta ataagccagt atgcggtcgc ggtagttacc ttcgccggat
    28441 tgcagcaact cagtcagtcg ctggtgatat gtggcgtaat ggcgctcaag catggcaacg
    28501 ccaaacgctt ctttagagcg aaagtagtga tagaaggacc ctttcggcac ttcagcggtt
    28561 tttagtaatt cgcttagccc catcccggtg aacccacgtt gcaggcaaag ttgctcgccc
    28621 gtcgccagga gatgttcgcg agtatcatgt tcggtgtgtt tgttcatgcg ttggagtgta
    28681 gtagaccagt cggtctagcg caagcaaagt tgctactttg ggcattaaca gcttcaataa
    28741 tggtaataat cttatttgag gagggcgaag tggcggagca attagagttc tttcccgtcc
    28801 agagcccgtg ccggggaatt tgccagtctg acgaacgcgg gttttgtcgc ggctgttttc
    28861 gtagtcggga tgagcgtttt aactggaata aaatgagcga tggcgaaaaa caagaggtac
    28921 tgcgcctttg tcggcagcgg ttaatgcgca aattacgtgc aaataagcca gcgtcatcag
    28981 acgaaccgga gcaaccatca ctcttttgag cctaatcttg cgtatactcc tctcatatct
    29041 gataagagga agcgattatg gttcagcgta ttactattgc gccgcaaggc ccggagtttt
    29101 cccgttttgt gatgggctac tggcgattga tggactggaa tatgtccgcc cgccagctgg
    29161 tcagttttat tgaagagcat ctggatctcg gcgtgaccac cgtggaccat gctgatattt
    29221 atggtggcta tcagtgcgaa gcggcgtttg gcgaggcgtt gaaactggca cctcacctgc
    29281 gtgaacggat ggaaatcgtc agtaaatgcg gtatcgcgac gaccgcgcgt gaagaaaacg
    29341 tcattggcca ttacattact gaccgcgatc acatcattaa gagcgccgaa cagtcgctaa
    29401 ttaatctcgc gaccgatcat ctggatctgc tgttaatcca ccgaccagac ccgttaatgg
    29461 atgccgatga agtggcggac gcgtttaaac atctgcatca gagcggcaaa gtgcgtcatt
    29521 ttggcgtatc gaactttacg cctgcgcaat ttgcgctttt gcaatcacgc ctgccgttta
    29581 cccttgccac taatcaggtg gaaatatccc cggtacatca gccgttactg ctggatggca
    29641 cgctcgacca actgcaacaa ctgcgtgttc gtccgatggc gtggtcctgc cttggtggtg
    29701 gtcgtctgtt taatgatgat tatttccagc cgctgcgtga tgaactggct gtggtggcag
    29761 aggagttaaa cgcgggctcg attgaacagg tggtttacgc ctgggtatta cgtttaccgt
    29821 cgcagccgct gccaattatc ggctcaggta aaattgagcg cgtacgggca gctgtcgaag
    29881 cagaaacact gaaaatgacc cgtcaacaat ggtttcgtat ccgtaaagcg gcactaggtt
    29941 acgacgtacc gtaagcactt ttaggaatag ccgccgttca aaaatgtgtc actggtttac
    30001 acttattcag gaatgcacaa tgaacggagg tcctatgaaa cgttttagtc tggccattct
    30061 ggcgctggtt gttgcgaccg gcgcacaagc tgccagtgaa aaagtcgaga tgaacctcgt
    30121 cacgtcgcaa ggggttgggc agtcaattgg tagcgtcacc attactgaaa ccgataaagg
    30181 tctggagttt tcgcccgatc tgaaagcatt acctcccggt gaacatggct tccatattca
    30241 tgccaaagga agctgccagc cagccaccaa agatggcaaa gccagcgccg cagaatccgc
    30301 aggcgggcat cttgatccac aaaataccgg taaacatgaa gggccagaag gtgccgggca
    30361 tttaggcgat ctgcctgcac tggtagtcaa taatgacggc aaggcgaccg atgccgtcat
    30421 cgcgcctcgt ctgaaatcac tggatgaagt caaagacaaa gcgctgatgg tccacgttgg
    30481 cggcgataat atgtccgatc aacccaaacc gctgggcggt ggcggtgaac gctatgcctg
    30541 tggtgtaatt aagtaattaa gaagccagcg taccttgcgg cggtgcttgc tcaagttgcg
    30601 aaagcgagca gtacagccgc cagacaattg ccgccagttc tcgggcggca ggttgatgat
    30661 gacgggcaag gcagtcgcaa attcgctgta attcttcaag tgtggccgcc agtgattttt
    30721 gctgaacgcc acgctcactc atcactcccc gcaacagtga aatacagtta tcccgtactc
    30781 gcgataacgg atcggaacgc gattcccagt cgcgcaattg ccagacaaca tgagaacagt
    30841 tcagcaacac tacaccccag cgtaataacc aacggcgagc cagcgcatcc tggctgttac
    30901 tcaactgact gacgtgatga taagtgagcg attcaaattc gctttcactc agtgttggat
    30961 ggcggcttag ttgatcgaca aaatcccggc gcagcgcgcg aatatggcgg cggcttttac
    31021 gagcatccga tcctggacgc agaatggcga acgctaacca cgccaacgcg acgccaacaa
    31081 tttttgccag attatcgtta agaaaatcag caaaatcata caccggggga ttagtgacag
    31141 cgataaacga acccataaaa acaatcagtt gcccccacag tgcggcaaat ttgggcatct
    31201 gcaatttaag aagctgcatt gtcgccagca gtggaaagag aaacagtaaa aattgccaca
    31261 gatcgctaat ctgcaccatc aggccaaatt tgaccacaaa gctgaatagc gaaagtaaca
    31321 ccagcgtgcg catcagcagt gacaacgact taaacggtgc cgcgacggcg gagtagagta
    31381 cgcagctaat tgctgccagc gttaaagcat tggcaccggc atcccattgc gaagcaatac
    31441 tccatgcgcc aatcatcatc aacgtacaaa atgtacgcag cccgctccac atagcttcgg
    31501 cgttatcggt atgacgagcc aggccggatg tccgtgggag tctggcacga tcatctacac
    31561 cgctttgcag acgatgtaat tcctgactac tttgcagata aaggcggcaa aaataacgta
    31621 gtcgctgcca gaaggcgacg tgccggtagt cggcgacgtt ggtcgggcgt agcggggaga
    31681 taatacgtgc gacggtgtaa acatctgttt gcgaactggc gagcgccgtc agcagctgtt
    31741 cgagaatttc tcgcgtggca tctggcggtg agggccagtt gagcaacata cgtcgcaggc
    31801 tggagatgac actggtcata cgtaattgct ggtggagtag cgcattaaga cgcgcgtttt
    31861 gctggcgaaa acgatagtgg ctccagaaag cctggatacg cagcaaattc atggtcagta
    31921 tctgcccaat caccccttca tgtgctgcac gaatggcatc ggttgtttca ggccgccaga
    31981 gtaaactggc atgttccagt aatcgggcgt gcatgttttt caatgcggtt aaaagggctg
    32041 tagcatcgga actgctcggc aggatcatca tcatcatgcc gccgcacaaa ataccgacta
    32101 tcacctcgca aacacgcgcc tgagcgatat cccacagctg gctggcctca gtaatattaa
    32161 ccatcggaaa ggcgatgatg gcagccgtgt agcccgccag ttgaaatgca tacgcgacgt
    32221 tattcgtgaa gtgcgcacag gcccaggtac aaaagccaag ccacgccgac atgctcaata
    32281 gaaaaaacca cggctcattg agcgtatgtc cggcaagaag cagtgccgca atggctccga
    32341 gcaaactgcc agcgatgcgt ccgaggcttt tgctgatgac accgccaacg gtgggaaagc
    32401 taaccaccgc agccgaggtc atcgcccaat agggctcatc aagattcaaa taataggcaa
    32461 ccgtcagcgc cagacacatg gcaatggtat tgcgtaacgc ataacgccat tgcgccagcg
    32521 tggccctgaa ccagggcaaa ttgcgcaagg accatgacga tgcgttcatt attgacctac
    32581 ggcaatgcta caggtggtgc ccgacaccag cgtgacatcg cctgggactt tatccagcgt
    32641 aaagcggacg ggaacgcgtt gggcgaggcg aacccagggg acgttaggtt tcacatccgg
    32701 aataaggctt gagtcacttt caacgctctg atcataaatc gcccgaccga tactggacgc
    32761 gtgaccctgt aacgttttgt tatcactata gagggtaatt tgtgctggtg caccttcgcg
    32821 gatatggcgc aatttggttt cttcaaaata accaatgacg taaaacgagt ggctgtcaac
    32881 aagggcaaac agcggtttcc cggtgtcagc gtagtcgccg atgcgggtag tgagattagt
    32941 cacccatcca ctcaccgggg cgcgaatttc cgtttgcgcc agttgccatt gtgcctgttt
    33001 tagcgtcgcc tgtgcggcat caacgctggc ctgcatcgct ttaacattga ggttagcggt
    33061 atcgagttct tcggcagaaa taaaattttg tgagagatga cgacgacgat tcgcttcgtt
    33121 attggctttt gcgaggtcag attgcgcttt cgccagctga gcctgtgcgt tcagctcggc
    33181 gatctgaaag ggcgttttat cgatcgtgag cagaagatcc cccgcgttaa ccagctggtt
    33241 gtcttttata ttcagctcaa caatacgtcc cgacacctgt ggggtgatag aaacctgttc
    33301 tgcgcgtatt ttcccgtcgc gtgtccacgg agattgcatg taaaaattcc acagccacca
    33361 tccggcaaga accgcaacta ccgctacaat gattgttgag aaatacttaa ttgttttaat
    33421 tgacatagtt accacgcaat cagtattgcc agagcaaggc aaacgcaaat cgcaaacagc
    33481 gataaatcca ttaacaaggg atgccagatg tcaccggcgt agatccagcc tcgaagcagg
    33541 cggtgtatga caagccagat gacgaatcca aatgcgaagg ctttgaaaaa cggaggaaag
    33601 tagacggacg caccgaacac cagatcttgt aagggcaatc ctgttgcatt gagcataaac
    33661 ttcacaaagt agattccttt acgacagaat atattgcgtt agattaataa atattcttta
    33721 agtgcgaaaa atttacgcgc aatttctgaa aaatatgtct gacggtaacc aaatgcagca
    33781 atacatttgt tttagcaata caattgctgc acactattct aaaagccgca taatatctta
    33841 gcaagctaat tataaggaga tgaaattgga atcgccacta ggttctgatc tggcacggtt
    33901 ggtgcgcata tggcgtgctc tgatagacca tcgcctgaaa ccgctggagt taacacaaac
    33961 ccattgggtt acgttacaca atatccatca gttacctcca gaccagtcgc aaattcaact
    34021 ggcaaaagcg attggcatcg agcagccatc actggtccgt acgctggacc aactggaaga
    34081 aaaagggtta atttcgcgtc aaacttgtgc cagcgatcgt cgggctaaac gtattaaact
    34141 gacggaaaag gcagagccgc tgatcagcga aatggaagct gttattaaca aaacccgcgc
    34201 ggaaatttta catggcatct ccgcagagga actggaacaa ctgattaagc tcatcgcaaa
    34261 acttgagcat aatatcattg agttacaggc caaagggtga aatgaagggg gcgtgtggtc
    34321 agtaacctga ccacacgcaa acttatttag cgcggggaaa cggtcacctg actgccattg
    34381 ctggccagta cgacacgttg gcctggagag aaatgagtgt tcccttgttt ctgtaccacc
    34441 atgatggtgt tgccatcatc tttacgaatt tccagctcga caccctgcgt tttgttcatt
    34501 gcactctgta cgccctgacc ggctacacca cctgcaacag cccctgctgc agtagccaga
    34561 gaacgcccgg ttccgccacc aatagtattt cccaggaaac caccaagtac agcaccgcca
    34621 attgcgccga taacgttgga atcatcaccg ccctgaatct gtacctgacg tacattaacg
    34681 atggtgccat agctgacatt ctgtacttgt ttcgcttcag aagcggtata aacatcccct
    34741 gacagggtgt cgttattaac acaaccgaca agagacagac ctaccattga aacaaccaat
    34801 acgcgtttaa tcattgaaaa atctcctgtt caccatgaaa cgccacgcaa gcatccctca
    34861 tggtcaaagt atatggcata tgtgaatcca tggttacatg ttctaccaaa tatgagcaaa
    34921 tcataatgaa agtaggatca accaattata aacaaaatat tcatgatgat tattgatttg
    34981 aagataatgt tttactacca acggtaaaaa ttggtcattc cgcatggtat tcacgaaaag
    35041 tttatgtttg agtggtgcgc aaacgtcaga ttgaaaaagg atgaactatg aaatcgggcc
    35101 gctttattgg cgttatgtca ggcaccagcc ttgatggtgt tgatgttgtg ttggcgacaa
    35161 ttgatgaaca ccgggtcgca cagctggcaa gtttgagctg gccgatcccg gtgtctctga
    35221 aacaggctgt actggatatt tgccagggcc agcagcttac actttcgcag tttggacagc
    35281 ttgatactca actcgggcga ctttttgctg atgcggtcaa tgccttgctt aaggaacaaa
    35341 acctgcaggc gagagatatc gttgcgatcg gctgtcacgg tcaaaccgtc tggcatgaac
    35401 cgacgggcgt ggcaccacac actttacaga ttggcgataa caatcaaatt gtggcgcgca
    35461 ccggaattac ggttgtcggt gattttcgcc gtcgcgatat tgccttggga ggacaaggtg
    35521 caccgctggt acctgcgttc catcatgcgc tgctggctca cccaaccgag cgacgaatgg
    35581 tgctcaatat tggcggcatc gccaatctgt cactgctcat tcctggccag ccggttgggg
    35641 gctacgatac cggtcctggt aacatgctga tggatgcctg gatctggcgt caggccggta
    35701 aaccttacga taaagatgcc gagtgggcac gggcgggtaa agttattctc ccactgctgc
    35761 aaaatatgct cagcgaccca tatttctcgc aacccgcacc gaaaagcacc ggacgcgaat
    35821 actttaacta cggctggctg gagcgccatt tgcgccattt tccgggggtt gatccccgtg
    35881 atgtgcaggc cacactggca gaactcaccg ccgtgaccat ttctgaacaa gttttgttga
    35941 gcggtggctg cgaacgattg atggtatgtg gtggaggtgg tcgtaatccg ctactcatgg
    36001 cgcgtctggc ggcattactg ccaggcacag aagtcaccac caccgatgcc gttggcatta
    36061 gtggcgatga catggaagca ttggcgttcg cctggcttgc ctggcgaacg ctggcgggat
    36121 taccaggaaa tctgccttcc gtcactggcg caagccagga gacggtactg ggggctattt
    36181 tccccgctaa cccgtgatat cagagttaac tgaatttttc ttaccgcgtc tgccgttaca
    36241 cttggaacgt tagggagggc gtattgccct ccagaccagg aaagtcttcg ggatatgtct
    36301 atgaaaaaac tgttactgat ctgtttgccg gtccttctca ccggctgtag cgcgttcaat
    36361 caactgtttg agcgcatgca aaccgatacg ctggaatacc agtgtgatga aaaaccgttg
    36421 acggtcaaac tgaataatcc gcgccaggaa gtcagttttg tttacgataa tcaactactg
    36481 catctcaaac agggcatttc agcctctggc gcgcgttaca ctgacggaat ctatgttttc
    36541 tggtcgaaag gcgatgaagc gactgtctat aaacgcgacc gcatcgtctt gaataactgt
    36601 cagttacaaa atccacagcg ttgagatttt tccaggggcg gcgcacaata gcgtcaccca
    36661 ctgacaatcc gtaaagaaaa ccatgtctga taacgacgaa ttgcagcaaa tcgcgcatct
    36721 gcgccgtgaa tacaccaaag gcgggttacg ccgccgcgat cttcctgccg agccattaac
    36781 cctttttgaa cgctggctct ctcaggcttg tgacgccaaa ctggcggacc ctaccgcgat
    36841 ggtggtcgct accgtggatg aacatggtca gccttatcag cgcatcgttt tactcaaaca
    36901 ttacgacgaa aaaggcatgg tgttttacac caacctcggc agccgtaaag cacatcaaat
    36961 cgaaaataat ccgcgcgtta gcctgctgtt cccgtggcat acccttgagc gccaggtgat
    37021 ggtgatcggt aaagcggaac gactttcgac tctcgaagtg atgaaatatt ttcatagtcg
    37081 cccacgtgat agccagattg gtgcatgggt ttcgaagcag tccagtcgca tttctgcccg
    37141 cggtatcctt gaaagtaaat tcctggagct gaagcagaag tttcaacagg gcgaagtgcc
    37201 attgccgagc ttttggggcg gttttcgcgt cagccttgaa cagattgagt tctggcaggg
    37261 cggtgagcat cgcctgcatg accgcttttt gtaccagcgt gaaaatgatg cgtggaagat
    37321 tgatcgtctt gcaccctgaa aagatgcaaa aatcttgctt taatcgctgg tactcctgat
    37381 tctggcactt tattctatgt ctctttcgca tctggcgaaa agtcgtgtac cggcaaaggt
    37441 gcagtcgtta tatacatgga gattttgatg gcaagcagta acttgattaa acaattgcaa
    37501 gagcgggggc tggtagccca ggtgacggac gaggaagcgt tagcagagcg actggcgcaa
    37561 ggcccgatcg cgctctattg cggcttcgat cctaccgctg acagcttgca tttggggcat
    37621 cttgttccat tgttatgcct gaaacgcttc cagcaggcgg gccacaagcc ggttgcgctg
    37681 gtaggcggcg cgacgggtct gattggcgac ccgagcttca aagctgctga gcgtaagctg
    37741 aacaccgaag aaactgttca ggagtgggtg gacaaaatcc gcaagcaggt tgccccgttc
    37801 ctcgatttcg actgtggaga aaactctgct atcgcggcga acaactatga ctggttcggc
    37861 aatatgaatg tgctgacctt cctgcgcgat attggcaaac acttctctgt taaccagatg
    37921 atcaacaaag aagcggttaa gcagcgtctc aaccgtgaag atcaggggat ttcgttcact
    37981 gagttttcct acaacctgtt gcagggttat gacttcgcct gtctgaacaa acagtacggt
    38041 gtggtgctgc aaattggtgg ttctgaccag tggggtaaca tcacttctgg tatcgacctg
    38101 acccgtcgtc tgcatcagaa tcaggtgttt ggcctgaccg ttccgctgat caccaaagca
    38161 gacggcacca aatttggtaa aactgaaggc ggcgcagtct ggttggatcc gaagaaaacc
    38221 agcccgtaca aattctacca gttctggatc aacactgcgg atgccgacgt ttaccgcttc
    38281 ctgaagttct tcaccttcat gagcattgaa gagatcaacg ccctggaaga agaagataaa
    38341 aacagcggta aagcaccgcg cgcccagtat gtgcttgcag aacaggtgac acgtctggtg
    38401 catggtgaag aaggtttgca ggcggcaaaa cgtattaccg aatgcctgtt cagcggttcc
    38461 ttgagtgcgc tgagtgaagc ggacttcgaa cagctggcgc aggacggcgt accgatggtt
    38521 gagatggaga agggcgcaga cctgatgcag gcgctggtcg attctgaact gcaaccttcc
    38581 cgtggtcagg cgcgtaaaac tatcgcctcc aatgccatca ccattaacgg tgaaaaacag
    38641 tccgatcctg aatacttctt taaagaagaa gatcgtctgt ttggtcgttt taccttattg
    38701 cgtcgcggca aaaagaatta ctgtctgatt tgctggaaat aatgcattaa gtggaaaggg
    38761 ggagtgagaa atcactcccc ctggttttta tacagggaac atgatgaaaa atattctcgc
    38821 tatccagtct cacgttgttt atggtcatgc gggtaacagt gcggcagagt ttccgatgcg
    38881 ccgcctgggc gcgaacgtct ggccgctgaa caccgttcaa ttttctaatc acactcaata
    38941 cggcaaatgg actggctgcg tgatgccgcc cagccattta accgaaattg tgcaaggcat
    39001 tgccgccatt gataaattac acacctgtga tgccgtatta agtggctatc tgggttcggc
    39061 ggagcagggt gaacatattc tcggtatcgt tcgtcaggtg aaagcagcga atccgcaggc
    39121 gaaatatttt tgcgatccgg taatgggtca tccggaaaaa ggctgtatcg ttgcaccggg
    39181 tgtcgcagag tttcatgtgc ggcacggttt gcctgccagc gatatcattg cgccgaatct
    39241 ggttgagctg gaaatactct gtgagcatgc ggtaaataac gtcgaagaag cggttctggc
    39301 agcgcgtgaa ctcattgcgc aagggccaca aattgtgttg gttaaacacc tggcgcgggc
    39361 tggctacagc cgtgaccgtt ttgaaatgct gctggttacc gccgatgaag cctggcatat
    39421 cagccgtccg ctggtggatt ttggtatgcg ccagccggta ggtgttggtg atgtgacgag
    39481 cggtttactg ctggtgaaac tgcttcaggg ggcaacgctg caggaggctc tggaacatgt
    39541 gaccgctgca gtatacgaaa tcatggtgac caccaaagca atgcaggaat atgagctgca
    39601 agtggttgct gctcaggatc gtattgccaa accagaacat tatttcagcg caacaaagct
    39661 ctgaaatatc tcatttcggc ccacaacgac ctgtgggccg atggctatta ctttaagcct
    39721 tccgctgaca gcgcgtcttg tacttccgga cgttcagcca tacgttgcat aaatgccgca
    39781 atgtgctcta acccttccag attcagtttc accgcgtatg cccagcgcag aaccgtaaac
    39841 agataggcat cagcaattgt aaatcgttgc ccgcagatcc agtgctcatc cttcagtgcc
    39901 tcgttcacat attgcagctt cttctccagc tgcgcgcgaa ctgtcgattt gtactcttcc
    39961 ggtgtatcag ggcgaaacag aggtgtgaaa cctttatgca gctcggtggc gatgtaattc
    40021 agccattcga tggttttata gcgggaaata ctgtttaccg gtgccagcaa ctggcggtcg
    40081 gggacgctgt cggcaagata ctgcataatc gctacgcctt ccgtcagcaa agtaccatcg
    40141 tccagcagca atgcaggtac ctgtccctta gggttaacgg caaagtaatc gtcaccgttt
    40201 tcgagacgtt ttttcattaa atccacactg acgagcgtaa aatcctttcc gctctcacgc
    40261 agggtgatat gggaagcgag agagcaggca cccggtttgt agaacaattt catcggtaac
    40321 tccttttgtc tgtggtttaa agtatggtag tgcgttgctg aacaaaaaaa aagccgctaa
    40381 cgaaaagtta gcggcttgta aaagagtttc cctgaatttt acgctacagc agctttcgcc
    40441 gctttgtctg cagcgtcatc ctgcgtcatg cggtgcagtt tcggcgcggt cagcagcatc
    40501 agtactgcaa taacggcagt agcgacacca atctgcaaga atacgcgacc atagacttcc
    40561 agtgacatca gcggatcggt aacgttatcc ggcacagcca tcatacccgc aacataacca
    40621 ccaatcaggt ttgcaccggc agtggtcagg aaccagctac ccataatgaa gcccatcaga
    40681 cgctgcggaa cgagttgtgc gaccattgcc agacccagac cagagatcat cagttccccg
    40741 atactctgca ggccatagct cgcgaccagc cagcttacag acacgatacc agcgtcagac
    40801 gcgaatttcg cacccagcgg cagaatcagg aacgcaccag aacacatcac catgccgatt
    40861 gcaaacttgg ttggcatcgg cagggtatcg cccatcttgt tatagatagc ggccagaatc
    40921 ggactaccga tgatgatcca gaacgggttc agtgcctgat actgctcagg ttctacggcc
    40981 agacccagaa tggagtgctc aacgttacga atcgcaaaga agttcagtga cgttggcatc
    41041 tggctgtaca gcacgaagaa gatgattgct tcgagcatca ggatgaaggc aacgatcatt
    41101 ttacgacgcg cagcaccttt catcgcgaaa gcttctttac cgaagataac cacaataccg
    41161 aaggcaacaa cgcccagcgc catacgcgca acttcctgat tgtgcagcag ccaggtggcg
    41221 atagcgatca gtgccacaac accaataatg gtcagcagca ggttacggta gttgataggc
    41281 tcgaagtctg gttttgaacc gtactgttta acccagcgtt ggcagaaggc gaagttaacg
    41341 atagtgatca gcaggcctac aacgctcaac gcaaacgcaa cactccagcc gtatttcgcg
    41401 gccagccacg gcgtagcaat catagagaag aaagagccga tgttgacgga catgtagtac
    41461 atggtgaatg caccgtccag acgcgggtcg tttttctcat agcatgtaga aagcagagaa
    41521 gacgggttag ctttaaacag gccgttaccg accgcaatag ccgccatacc catataaacg
    41581 ataccggcgt cgtgaccaga ccaggcaacc agagcataac caatcgccag cacaatagcg
    41641 ccgagcataa ttacgcgttt agtacccagt accttgtcac ctaaccagcc gccgatagcg
    41701 accagaccat aaaccagggc actaaaggaa gagaaaaggg tgattgaatc cgcttcagac
    41761 atacccagtt gtttaaccag gtaaacagcc ataattcctt gtaggccgta ataaccaaaa
    41821 cgttcccata actcaatcga gaagatgaga tagaacgcct tcggttgttt gaaagcgttc
    41881 aaactgacgc tttcagttgg tttttggttt gcagtggaca cttttacctc tttttttaca
    41941 tcccatatta acgggggtgc tttctcgcgt tatgcctaaa aaacatacgc gcaattgtta
    42001 tagtgggagg ggaaacggca ggtaatgttc actatcttgc actttcagac aagtcctttg
    42061 taataatctg ttacatataa ctgaggcggg gaattcaacc attccgggaa atgttatcca
    42121 gcgttaaatt tttcaaaaaa cgtaacggca gattttttca ctaccctgag agggggatga
    42181 tggcttaacg gcgatatgtt ctgctattta gcgtttttcc tagtgagata gtccagtttc
    42241 tgaaaaatag ccagtgtaat gttttgtagg tcaatatttg ttgcgtgtgt aaatttaaat
    42301 gattttaacc tagttcttgc aatgttttta cattgccaca gcatcgtgat tttgatcacg
    42361 aatgaataga aaatttcgct attaaaaaaa caattaacct gcatttttgg ttatccgcaa
    42421 acggattacc tgatgataac gctaagcaat ggcatcattg acgttaaaaa gatgttttaa
    42481 aagaggataa agaaaggtta tcaatggggt aatcggtgtt accccttatc attagatgtc
    42541 aactttctct ttgtattcac aaagatcttc aataatacaa gagccacagc ggggcttgcg
    42601 ggcaatgcag gtataacgcc cgtgcaggat caaccaatgg tggcagtcga ctttaaactc
    42661 tgccgggacc actttcagta gcttttcttc tacctgttcg acgtttttcc ccggcgcaaa
    42721 ttgagtacga ttacaaacgc ggaaaatgtg cgtatcgaca gcaatagtcg gccagccgaa
    42781 tgcagtgttt aatacgacgt tggctgtttt acgacctacg ccaggcaggg cttcaagcgc
    42841 agcacgatct tccggaacct cgccattatg ttgttccagt aagatacggc aggttttgat
    42901 gatattttct gctttgctgt tataaagccc aatggttttg atataggttt tcaccccttc
    42961 gacgcccagt tcaagcatcg ctggaggtgt attagccacc gggtagagtt tcgccgtcgc
    43021 cttattaaca ctgacatcgg tcgcctgagc ggaaagcagt acggcaatca gcaattcaaa
    43081 aggtgaactg aaattaagct cagtggtggg atgaggattg ttctcacgca ggcgagtgag
    43141 gatctccaga cgttttgctt tgttcatcag acattccctg tttcaccgtt tggcaatgca
    43201 cgttcggcag ctgcttcagt acggcgcttt ttcattcttt catcaatcag gtatttccct
    43261 gccagcatca gtcccaggcc aataaatgca cctggtggca gcatcgccag caggaaaggg
    43321 gagtcggtgt ggaaaatctc cacgcgtaat acttttgccc agctacctaa cagcgcatct
    43381 gcaccgtcaa acaacgtgcc attgcctatt atttcgcgta gtgaacccag cacgaacatg
    43441 gcgcaggttg cgcccatacc aattgaaaag ccgtccagtg ccgaaagcgc cggacctttt
    43501 ttggcggcga aggcttcagc gcggcccaca acgatacagt tagtgacaat cagcggaata
    43561 aaaatcccta atgattgata caggccaaag gcgtaggcgt tgatcagcat ctgtacagcg
    43621 ctgaccaccg aggcgatgat catcacgtaa atgggaatgc ggatctcggc tggcgtccag
    43681 tgacgcagcg tcgaaatggt caagttggtc agcgtcagta ccagcgtagt cgcaagtcct
    43741 aaacccaggg cgttggtggc agtggacgtg accgccagta gcggacaaag gccgagcaac
    43801 tggaccagcg cagagttgtt tttccacaac ccctgaacaa taacgtcttt aatttcgctc
    43861 acgctttatt ctccacaggc aggaagttga gaaagttgtg ccggtaacgt ctgagcgtac
    43921 aatccggcgc gttttaccgc atttaccacc gcgcgcggag taatcgtcgc gccggtgaac
    43981 tggtcgaaat caccaccatc tttcttcacc gcccagtgca catcatccgc accactgatt
    44041 tttttacccg caaaatgggt gatccagtca gaaaggcgca gttcgatttt atcgccaagc
    44101 cctggcgttt cgtggtgctc tgtcacgcgc gtgcctagta ccgtgccgtt aaaatcggct
    44161 cccaccagca gctgaatcgc accagaatag ccatctggcg cggttgtttc cagaacggcg
    44221 gctaccggtt tgtcatcctg tttggcgatg taaacccgat gctcaccttt acctaactct
    44281 ggcgcagtga ccagatagca actctgtgcc agcgcattgt tatagcgttc ggctggcagc
    44341 acttgatcaa ataacgcctt ttgttgcaga ctggcctgtt cagcaattgt cgttttggtc
    44401 atctggttga tggccgcagt taaccctgta gaacccgctg caaatagtgc cagtgtaatg
    44461 ccgtgttttc ggatagtttt cagcatggtt taccctttgc gatggccgta gacgcgcgga
    44521 cgcgtgtagt aatcgatcag aggaaccgtg atgttcgcca gcaggacggc aaaagccacg
    44581 ccgtcaggat agccgccgaa actgcggatc aaccagacta ataagcccgc cagcgcgccg
    44641 aaaataagac gaccacgatt ggtcgtagaa gcggtaaccg ggtcagtcaa aataaagaat
    44701 gcgccgagca tggtcgctcc agacagcaga tgaatttgcg gtgctgccag tgtttctggt
    44761 gagaacaacc agcccaacgt tgcgcataac gccagcgtta ctaagaagct gaggggaata
    44821 tgccagcgaa tcgctttctg ccatagcaac cacacgccgc cagccagcca ggcgagattt
    44881 acccattgcc aaccagcgcc cgccagaata ccgctgtaga tcggatattg cataatctgt
    44941 tcaaccgaat gaccggcacg aacagaggtt ttaaatgtat ccagcggtgt cgcctgacta
    45001 atgccatcaa tacctaagcg tagtgtgttc atatcaccac cactggcggt atgtccgctg
    45061 aaaataacct gtatggcgtc gataaaacca gggatgttga ccgcaattcc atgtggcggt
    45121 aaccagctgg tcatctgcac cgggaaggag atcagtaaga ccacataacc aatcattgcc
    45181 ggattaaacg ggttttgccc cagaccgcca tacaactgtt tagcgatgat caccgcaaac
    45241 accgtaccca gcacgaccat ccaccatggc gcgagggggg gaatacttac cgccagcaat
    45301 aagcctgtca gcaatgctga gttatctttc aacgttgcgg ctaccgactg cttgcgtagt
    45361 ttgagtacga gagcttcggc taacagagca ctgaccgacg ccaacaggat ctgaacgaga
    45421 gtaccccaac caaaaaacca cagttgcgct gcgattcctg gcacggctgc gagcaacacc
    45481 aacagcataa tgcgcgatgt ctggcgctgg ttatgggtat aaggggagct agctattctg
    45541 aataccattt agtcctcgtt tacaaccttt tgctgggcgg cttttttggc ctgaacgcgg
    45601 gcaatagccg cggcaactgc cgctttgcgc ggatcaacct gttcttctgg ttctgcgtta
    45661 gcctgttgct gttccagctt gcgtgctttg gcgcgtgcaa tagccgcttc gacggcgact
    45721 ttgcgcggat caacctgttc ttctggtacc gcattagcct gttgctgttc cagcttgcgt
    45781 gctttggcac gggcaatagc agcttcgacg gcagctttgc gcggatcgac ctgttcttct
    45841 ggttc
//
LOCUS       DAPHCV010000025        42854 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.025, whole genome shotgun sequence.
ACCESSION   DAPHCV010000025 DAPHCV010000000
VERSION     DAPHCV010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.025"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            137..472
                     /gene="eutS"
                     /locus_tag="RH033_001419"
     CDS             137..472
                     /gene="eutS"
                     /locus_tag="RH033_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HDV2990629.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            485..964
                     /gene="eutP"
                     /locus_tag="RH033_001420"
     CDS             485..964
                     /gene="eutP"
                     /locus_tag="RH033_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HDV2990630.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDNGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            939..1628
                     /gene="eutQ"
                     /locus_tag="RH033_001421"
     CDS             939..1628
                     /gene="eutQ"
                     /locus_tag="RH033_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HDV2990631.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECEESTPATVSVPVEKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQSLEQ
                     GAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAGFMQW
                     ENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSSVKFL
                     YVAWPANWQSL"
     gene            1625..2428
                     /gene="eutT"
                     /locus_tag="RH033_001422"
     CDS             1625..2428
                     /gene="eutT"
                     /locus_tag="RH033_001422"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HDV2990632.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLAFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLAC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            2425..3441
                     /gene="pta"
                     /locus_tag="RH033_001423"
     CDS             2425..3441
                     /gene="pta"
                     /locus_tag="RH033_001423"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDV2990633.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQLGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWQAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPQLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            3480..3773
                     /gene="eutM"
                     /locus_tag="RH033_001424"
     CDS             3480..3773
                     /gene="eutM"
                     /locus_tag="RH033_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HDV2990634.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            3880..4167
                     /gene="eutN"
                     /locus_tag="RH033_001425"
     CDS             3880..4167
                     /gene="eutN"
                     /locus_tag="RH033_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HDV2990635.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVTGGQVIFHK"
     gene            4179..5582
                     /locus_tag="RH033_001426"
     CDS             4179..5582
                     /locus_tag="RH033_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HDV2990636.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     IAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLKVPEQTRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            5593..6429
                     /gene="eutJ"
                     /locus_tag="RH033_001427"
     CDS             5593..6429
                     /gene="eutJ"
                     /locus_tag="RH033_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HDV2990637.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGLPVAVCLDWADVVREGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRIPLEEAEQYKRGHGEEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREEAEGLY
                     AK"
     gene            6419..7606
                     /gene="eutG"
                     /locus_tag="RH033_001428"
     CDS             6419..7606
                     /gene="eutG"
                     /locus_tag="RH033_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HDV2990638.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGAVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQSRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRDRFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            7823..9049
                     /gene="eutH"
                     /locus_tag="RH033_001429"
     CDS             7823..9049
                     /gene="eutH"
                     /locus_tag="RH033_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDV2990639.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVLIVAVLVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATAAKTEAEAQS"
     gene            9046..10449
                     /gene="eutA"
                     /locus_tag="RH033_001430"
     CDS             9046..10449
                     /gene="eutA"
                     /locus_tag="RH033_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HDV2990640.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMTLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALIQLDLCPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            10461..11822
                     /gene="eutB"
                     /locus_tag="RH033_001431"
     CDS             10461..11822
                     /gene="eutB"
                     /locus_tag="RH033_001431"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HDV2990641.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRSGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            11843..12730
                     /gene="eutC"
                     /locus_tag="RH033_001432"
     CDS             11843..12730
                     /gene="eutC"
                     /locus_tag="RH033_001432"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HDV2990642.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            12740..13399
                     /gene="eutL"
                     /locus_tag="RH033_001433"
     CDS             12740..13399
                     /gene="eutL"
                     /locus_tag="RH033_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HDV2990643.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            13412..13912
                     /gene="eutK"
                     /locus_tag="RH033_001434"
     CDS             13412..13912
                     /gene="eutK"
                     /locus_tag="RH033_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HDV2990644.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            13958..15010
                     /gene="eutR"
                     /locus_tag="RH033_001435"
     CDS             13958..15010
                     /gene="eutR"
                     /locus_tag="RH033_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HDV2990645.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMMTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKYAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(15016..15915)
                     /gene="hemF"
                     /locus_tag="RH033_001436"
     CDS             complement(15016..15915)
                     /gene="hemF"
                     /locus_tag="RH033_001436"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HDV2990646.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLSAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDEYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(15919..16788)
                     /gene="amiA"
                     /locus_tag="RH033_001437"
     CDS             complement(15919..16788)
                     /gene="amiA"
                     /locus_tag="RH033_001437"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HDV2990647.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSRKR"
     gene            17002..17427
                     /locus_tag="RH033_001438"
     CDS             17002..17427
                     /locus_tag="RH033_001438"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HDV2990648.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            17414..17863
                     /locus_tag="RH033_001439"
     CDS             17414..17863
                     /locus_tag="RH033_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HDV2990649.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRIWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            17924..18499
                     /locus_tag="RH033_001440"
     CDS             17924..18499
                     /locus_tag="RH033_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HDV2990650.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            18595..19494
                     /gene="yfeX"
                     /locus_tag="RH033_001441"
     CDS             18595..19494
                     /gene="yfeX"
                     /locus_tag="RH033_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HDV2990651.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(19552..20853)
                     /gene="pbp4b"
                     /locus_tag="RH033_001442"
     CDS             complement(19552..20853)
                     /gene="pbp4b"
                     /locus_tag="RH033_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HDV2990652.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESI
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFNAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQ"
     gene            complement(20858..22282)
                     /gene="murP"
                     /locus_tag="RH033_001443"
     CDS             complement(20858..22282)
                     /gene="murP"
                     /locus_tag="RH033_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HDV2990653.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(22286..23182)
                     /gene="murQ"
                     /locus_tag="RH033_001444"
     CDS             complement(22286..23182)
                     /gene="murQ"
                     /locus_tag="RH033_001444"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDV2990654.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPVVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCSAEQAEAALVACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            23354..24211
                     /gene="murR"
                     /locus_tag="RH033_001445"
     CDS             23354..24211
                     /gene="murR"
                     /locus_tag="RH033_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HDV2990655.1"
                     /translation="MLYLTKLRNAESDFTENEQKIADFLQARVSELKSVSSRNMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     TDSPLRRLAHYTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIER
                     SSELTQRLK"
     gene            24340..25131
                     /gene="ucpA"
                     /locus_tag="RH033_001446"
     CDS             24340..25131
                     /gene="ucpA"
                     /locus_tag="RH033_001446"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HDV2990656.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            25302..26318
                     /gene="cysP"
                     /locus_tag="RH033_001447"
     CDS             25302..26318
                     /gene="cysP"
                     /locus_tag="RH033_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HDV2990657.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            26318..27151
                     /gene="cysT"
                     /locus_tag="RH033_001448"
     CDS             26318..27151
                     /gene="cysT"
                     /locus_tag="RH033_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HDV2990658.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            27151..28026
                     /gene="cysW"
                     /locus_tag="RH033_001449"
     CDS             27151..28026
                     /gene="cysW"
                     /locus_tag="RH033_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HDV2990659.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            28016..29113
                     /gene="cysA"
                     /locus_tag="RH033_001450"
     CDS             28016..29113
                     /gene="cysA"
                     /locus_tag="RH033_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HDV2990660.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEALTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            29247..30158
                     /gene="cysM"
                     /locus_tag="RH033_001451"
     CDS             29247..30158
                     /gene="cysM"
                     /locus_tag="RH033_001451"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HDV2990661.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MLAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQSDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(30161..30529)
                     /locus_tag="RH033_001452"
     CDS             complement(30161..30529)
                     /locus_tag="RH033_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HDV2990662.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKMPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            30634..31485
                     /gene="pdxK"
                     /locus_tag="RH033_001453"
                     /gene_synonym="thiJ"
     CDS             30634..31485
                     /gene="pdxK"
                     /locus_tag="RH033_001453"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HDV2990663.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPNLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKELTDAVHSAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            complement(31527..32036)
                     /gene="crr"
                     /locus_tag="RH033_001454"
     CDS             complement(31527..32036)
                     /gene="crr"
                     /locus_tag="RH033_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDV2990664.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(32077..33804)
                     /gene="ptsI"
                     /locus_tag="RH033_001455"
     CDS             complement(32077..33804)
                     /gene="ptsI"
                     /locus_tag="RH033_001455"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HDV2990665.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(33849..34106)
                     /gene="ptsH"
                     /locus_tag="RH033_001456"
     CDS             complement(33849..34106)
                     /gene="ptsH"
                     /locus_tag="RH033_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HDV2990666.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(34490..35461)
                     /gene="cysK"
                     /locus_tag="RH033_001457"
     CDS             complement(34490..35461)
                     /gene="cysK"
                     /locus_tag="RH033_001457"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDV2990667.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            complement(35646..36407)
                     /gene="cysZ"
                     /locus_tag="RH033_001458"
     CDS             complement(35646..36407)
                     /gene="cysZ"
                     /locus_tag="RH033_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HDV2990668.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDMPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            36637..37635
                     /gene="zipA"
                     /locus_tag="RH033_001459"
     CDS             36637..37635
                     /gene="zipA"
                     /locus_tag="RH033_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HDV2990669.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPVQQPAYQPQPEQPLQQPVSPQVASAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            37706..39721
                     /gene="ligA"
                     /locus_tag="RH033_001460"
     CDS             37706..39721
                     /gene="ligA"
                     /locus_tag="RH033_001460"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDV2990670.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPVVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDETEMLRLLGS"
     gene            39723..39941
                     /locus_tag="RH033_001461"
     CDS             39723..39941
                     /locus_tag="RH033_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HDV2990671.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(39938..40936)
                     /locus_tag="RH033_001462"
     CDS             complement(39938..40936)
                     /locus_tag="RH033_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HDV2990672.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            41026..41952
                     /locus_tag="RH033_001463"
     CDS             41026..41952
                     /locus_tag="RH033_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2990673.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVSDLQCE
                     AILSEPFFLLCHRDSALAAEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAQALWEVVRGQGRALMAGREGDPLYQI"
     gene            complement(41990..42754)
                     /locus_tag="RH033_001464"
     CDS             complement(41990..42754)
                     /locus_tag="RH033_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2990674.1"
                     /translation="MKKHLFTLTLSSVLAIPAVSHAEFKGGFADIGIHYLDWTSRTTE
                     KSSTKSHKDDFGYLELEGGANFSWGEMYGFFDWENFYNDRHDKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYSFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
BASE COUNT         9973 a        10982 c        12030 g         9869 t
ORIGIN      
        1 aagtttatgc accacatcaa atatctctcc attcttagtg atctacctca ccttttaaac
       61 gcgcttgccg aattttgtta tttactctga cgaaaaattg tcacgataca cgaaagtttt
      121 tcacaggcgg cgactcatgg ataaagaacg catcattcag gaatttgtgc ccggtaaaca
      181 ggtcacactg gcgcatctca tcgcgcatcc cggcgaggaa ctggcgaaga aaatcggcgt
      241 tccggatgcg ggcgcaatcg gcattatgac gctaactccc ggagaaacgg cgatgatcgc
      301 cggtgattta gcgttgaaag ccgccgatgt gcatatcggt tttctcgacc gttttagcgg
      361 cgcgctggtg atttatggct cggtgggcgc agtagaagaa gcgttatcac aaaccgtcag
      421 tggtttagga cgcttattaa attatacgct ttgtgaaatg accaaaagtt aatacgaggc
      481 attcatgaaa cgaattgcgt ttgtgggtac cgtcggggca ggaaaaacaa cgttatttaa
      541 tgcgttgcag gggaattata ccctcgcaag aaaaacacag gccgtggaat ttaatgataa
      601 tggcgatatc gatacgccgg gtgaatattt tagccatccc cgctggtatc acgccttaat
      661 tactacgcta caggatgtgg acatgctgat ctatgtccac ggcgcgaatg acccggaaag
      721 tcggttgcct gccgggttgc tggatattgg cgtcagcaaa cggcaaatcg ccgtcatcag
      781 taaaacggac atgccagatg ctgatgtcgc cgcaacgcga aagttattgc ttgagacggg
      841 cttcgaagag ccgattttcg agctaaacag ccacgacccg caaagcgtgc agcaactggt
      901 ggattatctg gcatctctca ccaaacagga ggaagcaggt gaaaaaactc atcacagcga
      961 atgatattcg tgaagcacac gcgcgcggcg aacaggcaat gtccgtggtt ctgcgcgcca
     1021 gcattattac cccggaagcc cgtgaagtgg cggatctgtt gggttttacc atcaccgaat
     1081 gcgaggaatc gacaccagca acggtatctg tgcccgtaga gaaaaccgaa agccagcgta
     1141 tccgcgaaac cattatcgcc cagttaccgg aaggccagtt taccgaaagc ctggtcgcgc
     1201 agttgatgga aaaagtgatg aaggaaaaac agtcgctgga gcagggcgca atgcagccga
     1261 gcttcaaatc ggtgaccggc aaaggcggca tcaaagtgat tgatggcagt agcgtcaagt
     1321 ttggtcgttt cgatggcgca cagccacact gcgtcggatt aacggatttg gttaccggag
     1381 acgacggcag cagcatggcc gccggtttta tgcagtggga gaacgcattc ttcccgtgga
     1441 ctctgaacta cgacgaaatc gacatggtgc tggaagggga gctgcatgtt cgccacgaag
     1501 gcgaaaccat gatcgccaaa gcaggcgacg tgatgtttat cccgaaaggc tccagcatcg
     1561 aatttggtac gacatccagc gtgaaattcc tgtatgtcgc ctggccggct aactggcaat
     1621 ccttatgaaa gatttcatca ccgaagcatg gctaagagcg aaccatacgc tcagcgaagg
     1681 cgcagagatc catctccctg cggacagtcg cctgacgccc tctgcccggg agttactgga
     1741 aagccgccat ctgcgcatca agtttattga cgagcagggc cgcctgtttg ttgacgatga
     1801 acagcagcag ccgcagcccg ttcatgggct gaccagtagc gatgaacatc cgcaggcgtg
     1861 ctgcgaactg tgccgccagc cggtggcaaa aaagccggat acgctgactc acctgtcggc
     1921 ggaaaaaatg gttgccaaaa gcgatccgcg cctggctttt cgcgctgttc tcgacagcac
     1981 cattgcgctg gcagtgtggc tgcaaattga actggcggaa ccgtggcagc cgtggctggc
     2041 ggatatccgt tcgcgtcttg gcaacattat gcgtgccgat gcgctggggg aaccgctggc
     2101 ctgccaggcg attgtcgggc tttctgacga agatctgcat cggctttctc atcagccgct
     2161 gcgctatctc gaccacgatc atctggtgcc ggaagccagc cacgggcgcg atgcggcgtt
     2221 actcaatctg ctacgcacca aagtgcggga aaccgaaacc gtagcggcgc aggtctttat
     2281 cacccgcagc tttgaagtat tacgcccgga catcttgcag gcgctgaacc gcctttccag
     2341 cacggtctac gtgatgatga tcctgagcgt aacgaagcag ccgctaacgg tgaagcagat
     2401 ccaacaacga ctgggggaaa cgcaatgatt attgaacgtt gtcgtgaact ggcgttgcga
     2461 gcgcccgcca gagtagtttt tccggatgcg ttagatcaac gtgtgctgaa agccgcgcaa
     2521 tatttacatc aacaaggtct ggcaacgccc attctggtcg ccaatccgtt tgaacttcgt
     2581 cagtttgcgc tcagtcacgg cgtggcgatg gacgggctac aggtgattga tccgcatggc
     2641 aacctcgcaa tgcgggaaga atttgctcat cgctggctgg cccgcgcggg cgaaaaaacg
     2701 ccgccggatg cgctggaaaa acttaccgac ccgctgatgt tcgccgcagc aatggtcagc
     2761 gccggtaaag cggatgtctg tatcgcgggc aacctctctt ccacggcgaa tgtgctgcgt
     2821 gccggattac gcattatcgg cttgcagcta ggctgtaaaa cgctctcatc cattttcctg
     2881 atgctgccac agtacagcgg ccccgcgttg ggttttgccg attgcagcgt ggtgccacag
     2941 ccgacggcgg cacaactggc ggatatcgcc cttgccagcg ccgaaacctg gcaggccatc
     3001 accggagaag agccgcgcgt ggcgatgttg tcgttttcca gtaacggcag cgcccgtcac
     3061 ccctgcgttg ccaacgtcca gcaggcgaca gaaatcgtcc gtgagcgcgc gccgcagttg
     3121 gtagtcgatg gcgagttgca gtttgacgcc gccttcgtgc cggaagtggc ggcgcaaaaa
     3181 gcgcctgcca gcccgctaca gggcaaggcc aatgtgatgg tttttccgtc gctggaggcc
     3241 ggaaatattg gctacaaaat cgcacaacga cttggcggat atcgtgccgt cgggccattg
     3301 atacaaggac ttgccgcgcc gatgcacgat ctctctcgtg gttgtagcgt gcaggaaatt
     3361 atcgagctgg cgctggtggc agcagtgccg cgtcagacag aagtgaaccg cgaaagcagt
     3421 ttacaaacac tggttgaatg aacggtcccg ttctggaccc tgataaagag gaaagcacaa
     3481 tggaagcatt aggaatgatc gaaacccggg gcctggttgc actgattgag gcttctgacg
     3541 cgatggtgaa agctgcgcgc gtcaagctgg ttggtgttaa gcagattggc ggcggcctgt
     3601 gtactgccat ggttcgtggt gatgttgcag cctgtaaagc cgcgaccgac gcaggtgctg
     3661 cagcggcaca gcgtatcggc gagctggtat ctgtacacgt tatcccgcgt ccgcacggcg
     3721 acctggaaga agtcttcccg atcggcctga aaggcgatag cagcaacctg taatcaccct
     3781 gtgtgacgtc gattcgggcg tcacgctctc tcctttttca gtttaccttc cgcggagggt
     3841 agggggagct tttacctgaa atacgccacg gaggcgggta tgaaactggc agtcgtcact
     3901 ggacaaattg tttgtaccgt acgccatcac ggactggcgc atgacaaatt gctgatggtg
     3961 gaaatgattg atccacaagg taatcctgac gggcaatgcg ccgtcgccat cgacaatatt
     4021 ggcgcgggaa ccggggagtg ggtgttgctg gtgagtggca gttccgcccg ccaggcgcat
     4081 aaaagcgaaa cgtcaccggt cgatctgtgc gtgattggca tcgtcgatga ggtggtgact
     4141 ggcggtcagg tcattttcca caaataaggc agaacatcat gaatcaacag gatattgaac
     4201 aggtggtgaa agcggtacta ctgaaaatgc aaagcagtga tacgccgccc gccgccgttc
     4261 atgagatggg cgttttcgcg tccctggatg acgccgttgc ggcagccaaa attgcccagc
     4321 aagggttaaa gagcgtggca atgcgccagt tagccattgc tgccattcgt gaagcaggcg
     4381 aaaaacacgc cagagattta gcggaacttg ccgtcagtga aacgggtatg ggccgcgtag
     4441 aagataaatt tgccaaaaac gtcgctcagg cgcgcggcac gccaggcgtt gagtgccttt
     4501 ctccgcaggt gctgacaggc gacaacggcc tgactttaat tgaaaacgcg ccctggggcg
     4561 tagtggcttc ggtgacgcct tccactaacc cggcggcaac cgtcattaat aacgccatca
     4621 gcctgattgc cgcgggcaac agcgtcattt ttgccccgca tccggcggcg aaaaaagtct
     4681 ctcaacgggc tattacgttg ctcaaccagg cgattgttgc cgcaggcggg ccggaaaact
     4741 tactggttac tgtggcaaat ccggatatcg aaaccgcgca acgcttattc aagtttccgg
     4801 gcatcggcct tttggtggtg accggcggcg aagcggtagt agaagcggcg cgtaaacaca
     4861 ccaataaacg tctgattgcc gcaggcgctg gcaacccgcc ggtagtggtg gatgaaaccg
     4921 ccgacctcgc ccgtgccgct cagtccatcg tcaaaggcgc ttctttcgat aacaacatca
     4981 tttgtgccga cgaaaaggta ctgattgttg ttgatagcgt agccgatgaa ctgatgcgtc
     5041 tgatggaagg ccagcacgcg gtgaaactga ccgcagaaca ggcgcagcag ctgcaaccgg
     5101 tgttgctgaa aaatatcgac gagcgcggaa aaggcaccgt cagccgtgac tgggttggtc
     5161 gcgatgcggc gaaaatcgcg gcggcaatcg gccttaaagt tccggaacaa acgcgactgc
     5221 tgtttgtaga aaccaccgca gaacatccgt ttgctgtgac tgaactgatg atgccggtgc
     5281 tgcccgtcgt gcgcgtcgcc aacgtggcgg atgccattgc gctggcggtg aaactggaag
     5341 gcggttgcca ccacacggcg gcaatgcact cgcgcaatat cgagaacatg aaccagatgg
     5401 cgaatgccat tgataccagc attttcgtta agaacggacc gtgcattgcc gggctggggc
     5461 tgggcgggga aggctggacc accatgacca tcaccacgcc aaccggtgaa ggggtaacca
     5521 gcgcgcggac gtttgtccgt ctgcgtcgct gtgtattagt cgatgcgttt cgcattgttt
     5581 aaggagtaac cgatggcgca cgacgaacaa tggctcaccc cacgtctgca aacggcggca
     5641 acgctgtgta accagacgcc cgccgcgacg gaatctccgc tgtggctggg ggtggatctg
     5701 ggcacctgcg atgtggtgtc gatggttgtc gaccgcgacg gtctgccggt ggcggtatgc
     5761 ctcgactggg ccgatgtagt ccgcgagggc atcgtctggg atttcttcgg tgcggtcacc
     5821 attgttcgtc gccatctcga caccctcgag cagcaattcg gtcgccgttt tagccatgcg
     5881 gcgacctctt ttcctccagg tacggacccg cgtatttcca tcaacgtgct ggaatctgcc
     5941 gggctggaag tgagccatgt gctggatgag ccaaccgcag tcgcggatct gctgcaactg
     6001 gacaacgccg gtgtagtgga tatcggcggc ggcactaccg gcattgccat cgtgaaaaag
     6061 ggcaaggtga cgtactcggc ggatgaagcg actggcggac atcacatctc tctgactctc
     6121 gccggaaatc gtcgtattcc gctggaagag gcagagcagt acaagcgcgg tcacggtgaa
     6181 gagatttggc ctgcggtgaa accggtgtac gaaaaaatgg cggacattgt cgcccggcat
     6241 attgaagggc agggcattac tgatttatgg ctggcgggcg gctcttgtat gcaaccgggc
     6301 gtggcggaac tgtttcgcaa acaattcccg gcgttacagg tgcatttacc gcagcacagt
     6361 ttgtttatga ccccgctggc gatcgccagt agtgggcgag aggaagcgga ggggctatat
     6421 gcaaaatgaa ttgcagaccg cgctctttca ggcgttcgat accctgaatc tgcaacgggt
     6481 aaaaacattt agcgttccac cggtgacgct ttgcggtccg ggcgcggtga gtagttgcgg
     6541 gcagcaagcg caaacgcgtg ggctgaaaca tctgttcgtg atggcagaca gctttttgca
     6601 tcaggcgggg atgaccgccg ggctgacgcg cagcctggct gttaaaggca tcgccatgac
     6661 gctctggcca tgtccggtgg gcgaaccgtg catcaccgac gtgtgtgcag ccgtggcaca
     6721 gttgcgtgaa tcaggttgtg acggggtgat cgcgtttggc ggcggctcgg tgctggatgc
     6781 ggcgaaagcc gtggcgttgc tggtgacaaa cccggatagc acgctggcag aaatgtcaga
     6841 aaccagcgtt ctgcaatcgc gcttgccgct gattgccatt ccgactaccg ccggaaccgg
     6901 ctctgaaacc accaacgtaa cggtgattat cgacgcggtg agcgggcgca agcaggtgtt
     6961 agcccatgcc tcgctgatgc cggatgtggc gatcctcgac gccgcattga ccgaaggtgt
     7021 gccgtcgcat gtcacggcga tgaccggcat tgatgcgtta acccatgcca ttgaagcata
     7081 cagcgccctg aacgctacac cgtttaccga cagtctggcg attggtgcca ttgcgatgat
     7141 tggcaaatcg ctgccgaaag cggtgggcta cggtcacgac cttgccgcgc gcgagagcat
     7201 gttgctggct tcatgtatgg cgggaatggc gttttccagt gcgggtcttg ggttgtgcca
     7261 cgcgatggcg catcagccgg gcgcggcgct gcatattccg cacggtctcg cgaacgctat
     7321 gttgctgcca acggtgatgg agtttaaccg gatggtttgt cgtgatcggt ttagtcagat
     7381 tggtcgggcg ctgcgaacta aaaaatccga cgatcgtgac gctattaatg cggtaagtga
     7441 gctgatagcg gaagttggga ttggtaaacg actgggcgat gttggtgcga catctgcgca
     7501 ttacggcgca tgggcgcagg ccgcgctgga agatatttgt ctgcgcagta acccgcgtac
     7561 cgccagcctg gagcagattg tcggcctgta cgcagcggcg caataaatgc cggatgcgac
     7621 gcttgccgcg tcttatccgg cctacgggat tgcacatgta gggcggataa ggcgttcacg
     7681 ccgcatccgc cagtggcgcg gtgcaattgc cggatgcgac gcttgccgcg tcttatctgg
     7741 ccttcgggat tgcacatgta gggcggataa ggcgttcacg ccgcatccgc caataaataa
     7801 caactaacag ggagtaaagg cgatgggtat taacgaaatc atcatgtaca tcatgatgtt
     7861 ctttatgctg atagctgccg tagacaggat cctgtcgcag ttcggcggtt ctgctcgttt
     7921 cctcggtaag ttcggtaaaa gtatcgaagg atccggcggt cagttcgaag aaggctttat
     7981 ggcaatgggc gctctgggcc tggcgatggt cggtatgacc gcactggcac cggtactggc
     8041 ccacgttctc ggaccggtga ttatcccggt ttacgaaatg ctcggcgcaa acccgtcaat
     8101 gttcgccgga acactgctgg cgtgcgatat gggcggcttc ttcctcgcca aagagctggc
     8161 gggcggtgac gtagcagcgt ggctatactc tgggttaatt ctcgggtcga tgatggggcc
     8221 aacgattgtg ttttccattc cggtggcgct cggcattatc gaaccttctg accgtcgtta
     8281 tctggcgctc ggcgtgctgg cgggcattgt gaccattccg attggctgta ttgctggtgg
     8341 tctggttgct atgtactccg gtgtgcagat caacggtcag ccggtggaat tcacctttgc
     8401 gctgatcctg atgaacatga tcccggtact tatcgttgcg gtgctggtgg cgctggggct
     8461 gaaattcatc ccggaaaaaa tgatcaacgg cttccagatc ttcgccaaat tcctcgttgc
     8521 attgatcacc ctcggtcttg ctgccgcggt agtgaaattc ctccttggct gggaactgat
     8581 ccccggtctg gatcctatct ttatggcccc tggcgataaa cccggtgagg tgatgcgcgc
     8641 cattgaagtt atcggctcga tctcctgcgt tctgttaggg gcgtatccaa tggtactgct
     8701 gctgactcgc tggtttgaaa aaccgctgat gagcgtcggt aaagtgctga atatgaacaa
     8761 tatcgcggca gccggcatgg tggcaacgct tgccaacaac atcccaatgt ttggcatgat
     8821 gaagcagatg gatacccgcg gcaaagtcat caactgcgcc ttcgccgttt ccgctgcttt
     8881 cgccctgggt gaccacttag gctttgccgc tgccaacatg aacgccatga ttttcccgat
     8941 gattgtcggc aagttgatcg gcggcgtaac ggcgattggc gtggcgatga tgctggtgcc
     9001 aaaagaagac gcgaccgcgg ccaaaaccga agcggaggca caatcgtgaa cactcgccag
     9061 ctactgagcg tcggtatcga tatcggcacc accaccaccc aggtgatctt ctcccacctg
     9121 gagctggtta accgtgcggc ggtgtcgcag gtgccgcgct acgaattcat caaacgcgaa
     9181 attagctggc aaagcccggt gttctttacc cctgtcgata aacagggtgg tttaaaagaa
     9241 gcggaactga aaaccttaat cctcgagcaa tatcaggctg cgggcattgc gccggaaagc
     9301 gttgattctg gtgccatcat catcaccggt gaaagcgcga aaacccgcaa cgctcgcccg
     9361 gcggtgatga cgctctccca gtcgctgggc gattttgtcg tcgccagcgc cgggccgcat
     9421 ctcgaatccg tgatcgccgg tcacggtgcc ggggcgcaaa ccctttctga acaacggctg
     9481 tgtcgggtac tgaatatcga tatcggcggt ggcactgcga actacgccct gttcgatgcc
     9541 ggaaaaatca gcggcaccgc ctgcctcaat gtcggcggtc gcctgctgga aaccgacagc
     9601 caggggcgcg tggtttacgc tcataaaccg gggcagatga tagtggatga gtgcttcggt
     9661 gcaggcactg acgcccgttc gctgaccggc gcgcagctgg tgcaggttac ccggcggatg
     9721 gcagagctga ttgtcgaagt gattgacgga acgctctcgc cgctcgcgca ggcattaatg
     9781 caaaccggtt tgctgcccgc aggtgttacg cccgaaatca ttacgctttc tggcggcgtg
     9841 ggcgaatgtt atcgccacca gcccgccgac ccgttctgtt ttgccgatat cggcccgctt
     9901 ctggcaacgg cgctgcatga ccatccgcgc ctgcgtgaga tgaacgtgca gtttccggcg
     9961 caaaccgtgc gcgccacggt gattggtgcg ggtgcacata ccctttcact ctctggcagc
    10021 acgatctggc tggagggcgt acaactgccg ctgcgcaatt tgccggtggc gatcccgatt
    10081 gatgaaacgg atctggtgag tgcctggcaa caggcgctga ttcagctgga tctttgcccc
    10141 aaaactgacg cgtacgtgct ggcgcttccc gcctcgctgc ctgtgcgtta cgccgcggta
    10201 ctgacggtca tcaacgcgct ggtcgatttc gtcgcgcgtt ttccgaatcc gcatcccctg
    10261 ctggtggtgg ccgggcagga ctttggtaaa gctctgggca tgttgttgcg cccacagcta
    10321 caacaactcc cgttggcagt cattgacgaa gtgattgtcc gcgcggggga ctatatcgac
    10381 attggtacgc ctctttttgg cggatcggtt gtgccggtga cggtgaaatc actcgcattt
    10441 ccttcctgag ggaacgactt atgaaactaa agaccacatt gttcggcaat gtatatcagt
    10501 ttaaggatgt aaaagaggtg ctggctaaag ccaacgaact gcgttcgggg gatgtgctgg
    10561 cgggcgttgc agcggcaagc tcacaggagc gcgtggcggc aaagcaggtg ttgtcggaaa
    10621 tgaccgtagc ggacatccgc aataatccgg tgattgccta tgaagatgac tgcgtgacgc
    10681 ggctgattca ggacgacgtt aacgaaacgg cctacaacca gattaaaaac tggagcatca
    10741 gcgaactgcg tgaatacgtc ctgagcgatg aaaccagcgt ggacgacatt gcctttaccc
    10801 gcaaagggct gacctcggaa gtggtggcgg cggtagcgaa gatttgctcc aacgcggacc
    10861 tgatctacgg cgcgaagaaa atgccggtga tcaaaaaggc caataccacc atcggtattc
    10921 cgggcacctt tagcgcccgt ttgcagccga acgatacccg tgacgacgtg caaagtatcg
    10981 ccgcgcaaat ctacgaaggg ctttccttcg gggtgggcga tgcggtgatc ggcgttaacc
    11041 cggtgactga cgatgtggaa aacttaagcc gcgtgttgga caccatttac ggcgtgatcg
    11101 acaaattcaa cattccgacc cagggctgcg tgctggcgca cgtcaccacc cagattgaag
    11161 cgatccgtcg tggcgcacca ggcgggctga ttttccagag tatctgcggc agcgaaaaag
    11221 ggctgaaaga atttggcgtg gagctggcga tgctcgatga agcacgcgca gtgggcgcgg
    11281 agttcaaccg tatcgccggg gaaaactgcc tctacttcga aaccggacaa ggctctgcgc
    11341 tgtccgctgg cgctaacttc ggcgcagacc aggtgacgat ggaagcacgt aactatgggc
    11401 tggcgcgtca ttacgatccg tttatcgtca acaccgtggt cggctttatt gggccggagt
    11461 atctttacaa cgaccgccag attatccgtt cgggcttaga agatcacttt atgggtaagc
    11521 tgagcggcat ctctatgggc tgtgactgct gctacaccaa ccacgctgac gctgaccaga
    11581 acctcaacga aaacctcatg atcctgctcg ccaccgcagg ctgcaactac atcatgggga
    11641 tgccgctggg cgatgacatc atgctcaact atcagaccac ggcattccac gacactgcca
    11701 ctgtgcgtca gttactgaat ctgcgtccgt caccggagtt tgaacgctgg ctggaaagca
    11761 tgggcattat ggcaaacggt cgcctgacca aacgggcggg cgatccgtca ctgttcttct
    11821 gatgacgcgg agataacaca tcatggatca aaaacagatt gaagaaattg tacgcagcgt
    11881 gatggcgtca atgggacaag cggcccccgc gccgtcagaa gcaaagtgcg ccaccaccaa
    11941 ctgtgcggca ccggtgacct cggaaagctg cgcgctggat ttaggttctg cggaagcaaa
    12001 agcgtggatt ggtgtggaaa acccacatcg cgcggacgta ttaacagaac tgcgtcgcag
    12061 caccgtggcc cgcgtttgta ccggtcgtgc cggtccgcgt ccgcgtaccc aggcgttgct
    12121 gcgtttcctg gccgatcact ctcgttcgaa agataccgta ctgaaagaag taccggaaga
    12181 gtgggtgaaa gcgcagggct tgctggaagt ccgctccgaa atcagcgaca aaaatctcta
    12241 tctgactcgc ccggatatgg gccgccgcct gtgtgcagaa gctgttgaag cgctgaaagc
    12301 acagtgtgtt gctaatccgg acgtgcaggt cgttatttct gatggcctgt caactgatgc
    12361 gatcaccgtg aactacgaag agatcctgcc accgctgatg gcgggcctga aacaggccgg
    12421 gctgaaagtc ggtacgccat tctttgttcg ttatggtcgc gtgaagattg aagatcagat
    12481 cggtgagatc ctcggcgcga aagtggtgat cctgctggtg ggggaacgcc cgggattagg
    12541 gcagtcagaa agtctctcct gctacgccgt ttactcgccg cgtatggcga ccaccgttga
    12601 ggccgatcgc acctgcatct ctaacattca ccagggcggc acgccgccgg ttgaagccgc
    12661 agccgtcatt gtggatttgg cgaaacgtat gctggagcag aaagcatccg gcatcaacat
    12721 gacccgataa ggaggcatca tgccagcttt agatttgatt cgaccgtcgg tcaccgccat
    12781 gcgggtgatt gcctctgtta acgccgaatt tgcgcgtgaa ctgaaattgc cgccgcatat
    12841 tcgtagcctt gggctgattt ctgctgattc cgatgacgtc acctacattg cggctgacga
    12901 agcgaccaag caggcgatgg tcgaagtggt gtatggccgc tcgctgtatg cgggagctgc
    12961 acacggaccg tcaccgactg ccggtgaagt gctgattatg ctcggtgggc caaacccggc
    13021 ggaagtgcgt gctggtctgg atgcgatggt tgcgcatatt gaaaatggcg cggctttcca
    13081 gtgggctaat gacgcagaaa acacagcctt cctggcacat gtagtttcgc gtaccggttc
    13141 ttatctctca tcaaccgccg ggatcacgct tggcgatccg atggcgtatc tggtggcacc
    13201 gccgctggaa gcgacctatg gcattgatgc agccctgaaa tctgccgacg tgcaactggt
    13261 gacctacgta ccgccgccgt ctgaaaccaa ctactcggca gcatttttaa ccggtagcca
    13321 ggccgcgtgt aaagcagcct gtaacgcctt taccgatgca gtgctggaaa tcgcgcgtaa
    13381 tccaatccag cgtgcgtaac ggaggttgcc gatgatcaat gcactgggat tgctggaagt
    13441 ggacggcatg gtcgccgcga tagatgcagc tgatgccatg ctcaaagcag ctaacgttcg
    13501 tctgctcagt cacgaagtgc ttgaccctgg tcgcctaacg ctggtggtgg aaggcgatct
    13561 ggcggcgtgt cgtgcggcgc tggacgcagg ttgtgctgcc gcgatgcgta ccgggcgtgt
    13621 catcagccgt aaggagatcg gtcggccaga cgatgacacc cagtggctgg ttactggctt
    13681 taaccgccag ccgaagcaac ccgtaaagga acccgacgcg ccagttatcg tcgcggaatc
    13741 tgctgacgag ttgttggcgc tgttaacatc agtacgtcag ggaatgacgg caggagaagt
    13801 ggctgcccac tttggctggc cgctggaaaa agccagaaat gcgctcgaac agctcttttc
    13861 tgctgggacg ttacgtaaac gcagtagtcg ttatcgtctc aagccccatt aacctgtcgg
    13921 aggtgccggg tgtcctacaa cacccggcat taacatcatg aaaaagaccc gtacagccaa
    13981 tttgcaccat ctttatcatg aacccttacc cgaaaacctg aagctcacgc cgaaggtcga
    14041 agtggataat gttcatcaac gacagacaac ggatgtctat gaacatgctt tgacaattac
    14101 cgcctggcag cagatttacg atcagctgca tccgggcaag tttcatggtg aatttacgga
    14161 aattctactc gatgatattc aggtttttcg tgaatacacc ggtctggcac tgcgtcagtc
    14221 gtgcctggtc tggccgaact cgttctggtt tggcattccg gcgacgcgcg gtgagcaggg
    14281 atttatcggt tcgcaatgtc tgggaagcgc agaaatagcg acgcgccctg gtggaaccga
    14341 gtttgaatta agtacgccgg acgattacac gatcctgggc gtagtgcttt ctgaagatgt
    14401 catcacccgt caggctaact ttttgcataa tccggatcgg gtattacata tgctgcgtag
    14461 ccagtcggcg ttggaagtga aagagcagca taaagccgcg ctgtggggct ttgtccaaca
    14521 ggcgctggcg acgttttgcg agaacccgga aaatctccat cagcctgcag tgcgaaaagt
    14581 gctgggggat aatttgctaa tggcgatggg ggcgatgctg gaagaagcgc agccgatgat
    14641 gacggcggaa agcatcagtc atcagagtta ccgtcgattg ctttcccgcg cccgtgaata
    14701 tgtgctggaa aacatgtccg aaccggtgac ggtgctggac ttgtgtaatc aactgcatgt
    14761 cagccgccgc acgctacaaa acgcgtttca cgccattttg ggcattggtc cgaacgcgtg
    14821 gctgaaacgt attcgcctga acgccgtgcg ccgcgaactc ataagtccgt ggtcacaaag
    14881 cacaacggta aaatacgccg ccatgcagtg gggattctgg catcttgggc aatttgccac
    14941 ggattatcag caactgtttg ccgagaagcc gtcactgacg ctgcatcagc ggatgcgaga
    15001 gtgggggtga gggagttaca cccaatccct gaccttaata aactcactta aagccgcttc
    15061 cgggctgcca tcttttggct gataatcata ctcccagcgt accagtggcg gcattgacat
    15121 caggatcgac tcggtgcgcc cgccagtttg caggccaaac agcgtgccgc gatcccagac
    15181 cagattgaac tcgacataac gaccgcgacg gtagagctga aaattgcgct cgcgctcgcc
    15241 gtaggccatc gcttttcgtc gttctacaat tggtaaataa gcgtcggtgt agcctttgcc
    15301 taccgcctgc ataaaggcaa aacagtggtc gaaatctggc gtgttcagat catcaaagaa
    15361 cagcccgcca atgccgcgct gttcgttacg atgtttgagg tagaagtatt cgtcgcacca
    15421 ttttttgtaa cgcggataaa cgtcttcgcc aaatggcagg cacaggtcac gggcggtgcg
    15481 gtgccagtga atggcgtctt cttcaaaacc atagaaaggg gttaaatcga agccgccgcc
    15541 aaaccaccag acgggctctg cacccggctt ttcggcaata aaaaagcgca cattcgcgtg
    15601 gctggtgggg acatacggat tatgcggatg cactaccagt gaaacgccca tcgcctcgaa
    15661 actgcgcccg gcaagttccg gacgatgagc ggtggcggaa gcaggcatcg cctcaccgtg
    15721 gacatgtgaa aagttgacgc ctgcctgttc gaaaacacca ccattacgca aaacccgact
    15781 acgcccgccg ccgccagctt cgcgctgcca gctatcttcg acaaattctg cgccatcgac
    15841 ggcgctcaac tgctgacaaa tcgtatcctg aaggttgagc aggaactgtt taacctggtg
    15901 tgcgtcgggt ttcatcactt accgctttct cgagtgtgct ttctggttgt caaaccagtg
    15961 gaaataactg atcacgcctt cggcaatcgc tgtggcgatt ttctggcgaa atgctgccgt
    16021 gcctaacagc cgctcttctt ccgggttggt gataaacgag gtttccacca gcaccgaagg
    16081 aaccgacggc gatttcaaca ccacaaacgc ggcttgttcg gtgttgcggc tgtgcagttt
    16141 atgcaccggc ttaatcttct tcagaatatg cgagccgagc gtcaggctgt ttttaatagt
    16201 atcggtttgc accagatcaa acagcacttg ctgcaatagg tgatccttgt cagtcgcctt
    16261 tttaccggca acttcatcgg cgcggttttc gcgttcagac aggtatttcg ccattgcgct
    16321 acttgcccca cggttagaga gggcaaatac cgaagcaccg gcagctttcg ggttggtaaa
    16381 gccatcggca tgaattgaca taaacagatc tgcgccatgt ttatgggcga tttcaacgcg
    16441 atcgtaaagt gggataaacg tatcgccaga acgcgttaaa cgcgcatcaa tcccatgatt
    16501 acgcaaaatg gaacggacgt ttttagcaat cgccagcacc acatgttttt ctttcgaacc
    16561 gttgcgcccg atcgctccgg tatcaatccc gccgtggcct ggatcgagaa caacgacacg
    16621 tttgccgcca gattttttgg ctttcggctt gctgtgtccg ttgctggttt ttaaaggttc
    16681 gtctttggcg atggcttgcg acattcctga caacgtcagg gcagccaaac cggctttcag
    16741 cacctggcgg cgcgaagtga gtgtttttag tggtttaaaa gtgctcatac ggcctgagtt
    16801 gtaataataa agttccagat gttatatcgt atcgcgtaat ccgttacgac cgtttgctgt
    16861 tgagaattgt tttacttttc atttcaatag ttgacaactc cccattatgc cactttttca
    16921 accgattttc gtcagatatt ggctaattcg accgttcacg ccataatcag ccttttaacc
    16981 cgcaaaaagg taacgaatac catggagata cgcgtatttc gccaggaaga tttcgaagag
    17041 gtcatcaccc tttgggagcg ttgcgacttg ctgcgtccgt ggaacgatcc ggaaatggac
    17101 atcgagcgta agatgaacca tgacgtcagt ttgtttctgg tcgctgaggt aaacggtgaa
    17161 gtggtcggaa cggtgatggg cggttatgac gggcatcgcg ggtctgctta ttatcttggc
    17221 gtgcatccag agtttcgtgg gcgtgggatt gctaatgcgt tgcttaatcg gctggagaaa
    17281 aagctgattg ctcgcggctg tccgaaaatt cagatcaacg tgccggaaga taacgacatg
    17341 gtgctcggaa tgtatgaacg cctgggatat gaacacgccg acgtgctgag tctgggtaag
    17401 cgtttgattg aagatgaaga gtactgagtt tcatcctgtc cattatgatg cgcacggtcg
    17461 cctgcgttta cccttgctct tctggcttgt gctattgctt caggcgcgaa cctgggtgct
    17521 gtttgtcatt gctggtgcgt cgcgcgagca gggtactgcg ctgctaaatc tgttttatcc
    17581 cgatcacgat aatttctggc tggggttgat tcccggcatt ccggcggtgc tggcgttttt
    17641 gctgagtggt cggcgggctt catttccccg tatctggcat gtgctctatt ttctgttgct
    17701 gttggcgcag gtggttttac tttgctggca accgtggctg tggctgaacg gtgaatccgt
    17761 tagcggtatc gggctggcgc tggtagtggc ggatattgtg gcgttaatct ggctgctgac
    17821 caatcgacgt ttacgcgctt gttttaatga agaaaaagaa taacggcact ttttggtgaa
    17881 tttgcactcc aagcaacgtt attgaataac caaaggaagt gacatgaaat cgctgcgttt
    17941 aatgttatgc gctatgccgt tgatgctgac cggctgttcc acgatgtcgt cagttaactg
    18001 gtctgccgct aacccgtgga actggtttgg gtcgtccacc aaagtgagcg agcagggcgt
    18061 tggtgaatta acggcgtcca caccactgca agaacaagcc attgccgatg cgcttgatgg
    18121 cgattatcgc ctgcgcagcg gaatgaaaac cgcgaacggc aacgtggtgc gcttttttga
    18181 agtgatgaaa ggcgacaacg tggcgatggt gattaacggc gatcagggta cgatcagccg
    18241 cattgatgtg ctggatagcg atattcctgc tgacaccggt gttaaaatcg gtacaccgtt
    18301 tagcgacctt tacagcaaag catttggcaa ttgccaaaaa gctgatggtg atgataatcg
    18361 tgccgtcgaa tgtaaagccg aaggcagtca acatattagc taccagttca gcggggaatg
    18421 gagtggtcct gaagggttaa tgccttcaga cgataccctg aaaaactgga aagtcagtaa
    18481 aattatctgg cggcgttaat ttgcgtctga acaaaccgcc gccgcgaaaa aacacgtaaa
    18541 atagcgccca acaatgccac ggattgcgtg gcattcttat tttcaggagg aacaatgtct
    18601 caggttcaga gtggcatttt gccagaacat tgccgcgcgg cgatttggat cgaagccaac
    18661 gtgaaagggg aagttgacgc cctgcgtgcg gccagtaaaa catttgccga taaactggca
    18721 acttttgaag cgaaattccc ggatgcgcat cttggtgcgg tggttgcctt tggtaacaac
    18781 acctggcgcg ctctgagcgg cggcgttggg gccgaagagc tgaaagattt tccgggctac
    18841 ggtaaaggcc ttgcgccgac gacccagttc gatgtgttga tccacattct ttctctgcgt
    18901 cacgacgtaa acttctctgt cgctcaggcg gcgatggaag cctttggtga ctgcattgaa
    18961 gtgaaagaag agatccacgg cttccgttgg gttgaagagc gtgacctgag cggcttcgtt
    19021 gacggtactg aaaacccggc gggtgaagag acgcgccgcg aagtggcggt tatcaaagac
    19081 ggcgtggatg caggcggcag ctatgtgttt gtgcagcgtt gggagcacaa cctcaagcag
    19141 ctcaaccgga tgagcgttca cgatcaggag atgatgatcg ggcgcaccaa agaggccaac
    19201 gaagagattg acggcgacga acgtccggaa acctctcacc tgacccgcgt tgatctgaaa
    19261 gaagatggca aagggctgaa gattgttcgc cagagcctgc cgtacggcac cgccagtggc
    19321 actcacggtc tgtacttctg cgcctactgc gcgcgtctgc ataacattga gcagcaactg
    19381 ctgagcatgt ttggcgatac cgatggcaaa cgtgatgcga tgttgcgttt caccaaaccg
    19441 gtcactggcg gctattactt cgcgccgtcg ctggacaagt tgatggcgtt gtaatcgttt
    19501 ctgattcgat gatttaaggc cagccagtat cacaactgac tggccttttt attactgctt
    19561 taacgccgca tacacctgat caactaccca accataagtt gcaatcggta gctgaccgct
    19621 ttcgaacata ttgggatttt tttgtggatc ggcaactggc gaatgtggct tgttgcttaa
    19681 catcacaatc gccatatgat tcaccggatc gataacggtc accgtcccgg tccagccagt
    19741 gtgaccgtag gtttgcgggc ttgccagcgt gccaaacgtc ggcgtcatgg tggcattacc
    19801 attcacgcgc cagccaaggc caaaagtggc atcttcctta gagctggtgg tgaacatctt
    19861 caccgtttct gcattgaaca gctgcacatc gccatagccg ccgccgttca gcatcgtttg
    19921 cattaacacc gcaatatcgc cggtattgga aaacaaacct gcgtgcccgg aaacgccgcc
    19981 catcgaatag aatgcttttt catcgtgcac ctgaccccag agagtggagg tgcggatatt
    20041 cgggaagtga atcacgccat cgcgggtatt gccgttgagt tccgtggcgg caatttgctg
    20101 tggtttaaag cctttcagca gtggattaaa taccgtgtgc gtcaggccga gcgggcgata
    20161 aatcgactct tcaacatagc ggtcaagtgg ctgaccggta atcgactcga cgataaatcc
    20221 aagcagcata taatcgacat cgctgtagat atgcttgctg ccgggctgat actccagcgg
    20281 cgtgcgcttg atcatctcca gcgtctggcc tttatcctgg gaatataacg cgcccgcgac
    20341 cgctttattc gggtattgcg gatctgccgg gaaaccgccg ctgtgatgca gcaggtcaga
    20401 aatccgcagc gtgtttttgc ctttgatggt gtcatttgga ctatcggcaa atcccggaat
    20461 atatttcgca atccgatcgt caggatgcag cttgccttcg ctcattagct tttgcagagc
    20521 aaagttagtg gcgtacatct tggtattgga agccaggtca tacagcgtcc cggtggtggc
    20581 tttgacaggc tgttccatca atacgctgcc atcgtacttt ttcgccgctc cccaggcttt
    20641 gcgatacaca atctgattat cttttataat cagcaggttt acgccgggat aaccggcgtc
    20701 cacttgctgg ctaatccagc gatccatctg gttaagccgt tcgacgttga accctgcttt
    20761 ctctggcgaa ctttctgtca gtacagggta ttttgcggcg ctgacgctac aactaacagc
    20821 cagcagagaa agatagagca ttgtccgttt catattatca gtccagattg acgttacggc
    20881 agccaaagag cgtggtgaaa ataaacccgc aaacccatgc caccagaatc ccgcctgcat
    20941 aaaccgccat tgccggaagg atgccttgtg cggaagtcat cagcggcagc gccaccagac
    21001 cagacggccc aaaggcactg tttaagccca tcggcaggcc ccaccaggct atcagcccga
    21061 taaacaaacc acccgctgcg ccacctaaac aggcggtaac aaacggtttc atgcggggca
    21121 gggtgacgcc gtaaatcagc ggttcgccaa cacccagcag accgggaata atcgccccgc
    21181 gtacctgact gcgtaatgcg ctatgcggtt gcgcccgcca gtagagtgcc agcgccgcgc
    21241 ccacctggcc cgcgcctgcc attgaaagga tgggaaataa gctgttgaat ccctgactat
    21301 ccattaacgc gaggtaaacg ggaataaagc cctgatgcac gccaaacacc acggcgatca
    21361 ggaacagccc ggctaatact gcacaaccga gcggattact gttcaggtgc ataaacagcc
    21421 acgacatgcc ttcaaacagc cagccgccca gcggcataat gatcaggtag gccagcgtag
    21481 cggtgatcag cagggtgatt aacgaggtca acagcatgtc gagatcgtcc ggcataaagc
    21541 ggcgcaccat gccttcgatg cgggcgcagg cccacgcggc aatcaacacg ccgataatat
    21601 tgccgcgcgg atcgataggc agaccgaaga aatcgtgaaa tccggcgtag taaccagtgg
    21661 tggcagcagg gttataaccg agcaaaaaga gcgcggcgat aattgcgcca ttgacgcccg
    21721 tgccgccgaa cgcctgggcc gcgttatagc ccaccagaat caccaggaag gtgaacaaac
    21781 ctttgctgaa caccttcata aaattcagcg cgtcgggtag cgtgccctga gcatctgccg
    21841 gaacgtgcat caccgtggca attaacgtcg ctatccccag cagcagaccg gcggcaataa
    21901 aaccggggat cagcggcgtg aagatggtgg cgaatttggc gagaaattgt tgcacgccgg
    21961 aggtttgttt ggcttttaac tgacgtttat tctgggcggc gatttctgcg gcatcctgca
    22021 cgggtgcttc gcccagcaac tcgctcatgg cttttgcggc acgatgcgct ttgcccggcc
    22081 caaaaaccac ctgcacctga tcgctggtca aaatgacgcc cttcacgcct tccagcgttt
    22141 tgatattggg atcaaccagt gaactgtcat gtacacccag acgcaggcgc gtcatacagt
    22201 taccacaact ggcgatattt cccggtccgc cgacacgggt aagaatggtg ttcagaagtt
    22261 cactgctgat ctctttggcc atgggttatt ccttgtctaa aacctgacga ataaagccgc
    22321 cgtgttgatc aaggcgtttt ttagcttctg cggcatcgag atttttcagc accatcacaa
    22381 tggccgtttt acagttgcgc tcgcaggcaa ccaatgccgc ttccgcttgc tctgcgctac
    22441 atccggtagc gtttttgacg atattgacct ggcgcacatg caatttttcg ttggtggcga
    22501 ccacatcgac catcaggttg ccgaacactt tgccggattt aatcatcagc ccggtggaga
    22561 gcatattgag caccagtttc tgcgccgtac ctgctttcat tcgcgaagaa cctgtgacca
    22621 cttcggcccc aacaactggc gtaatggcaa actcagcggt ggttgaaacg gcgctcccgg
    22681 gattacagga aattcccact gtgcggcagc cgagctggcg tgcgtattcc agtccggcaa
    22741 ttacatacgg cgtgcgacca ctggccgcaa tgccaaccac cacatcctgc gcggttaagc
    22801 cgatgttttt caaatcatta acaccacctt cccggctatc ttcagcgcct tccaccgcgt
    22861 gctgaatggc atattcgcct ccagcaatca aaccaaccac cagacccggt ttaacgccgt
    22921 aggtgggcgg acattcgctg gcatccagaa tccccagacg accggatgtt cccgcaccga
    22981 gataaatcag acgcccgccg ccgcttacct gggcgtggat aacatcgatc gccgcggcga
    23041 tatccggcag tacgcgctca acggcgagtg gtacagtttt atcttcatcg ttgataatcc
    23101 ggcacatttc cagcgtcgat acgcggtcaa tttcagccga ggcggcgttc gagccttcag
    23161 taatcatctt ttcaagttgc atcggtggtg accttactat ttacgatcaa ataattgcag
    23221 atgtatggaa tcatatatct caatttgcta gttttaaagg aatcattgag tctgaaatca
    23281 cagagtgtga agagcgtcaa agggcaaaat ccgtgtgcta tgctgcgcgc aggatgtggt
    23341 gagggagtgg aagatgttgt acctgacaaa attacgcaat gcagaaagtg actttactga
    23401 aaatgagcaa aagatagccg actttctgca agccagggtg agcgaactga aatccgtttc
    23461 ttcacgaaat atggcgaaac agctcggtat tagccagtcg agtattgtga agtttgccca
    23521 gaagctcggc gcgcaaggat ttactgaatt gcgtatggcg ttaattggcg aatacagtgc
    23581 tagccgggag aaaacgaatg ccacggcgct gcacctgcac agttcgatta ccagtgatga
    23641 ttcgctggaa gtgattgccc gcaaattgaa tcgcgaaaaa gagctggcgc tggagcaaac
    23701 ctgtgcgttg ttcgactacg cgcggttgca aaaaatcatt gaggtcatca gtaaagcacc
    23761 gtttatccag ataacgggcc tgggtgggtc ggcgctggtg gggcgcgatc tttcattcaa
    23821 actgatgaaa attggttatc gtgtggcctg tgaagctgac acgcatgtac aagccaccgt
    23881 ttcccaggcg ctgaaaaaag gcgatgtaca aatcgctatc tcttacagcg gcagtaaaaa
    23941 agagattgtc ttgtgcgccg aggcagcacg aaagcagggg gcaacggtga ttgccattac
    24001 ctcgttaacc gattccccgc tgcggcggct ggcgcattac accctcgata ccgtttctgg
    24061 cgaaaccgaa tggcgcagct cgtcgatgtc cacccgcacg gcgcaaaatt ctgttaccga
    24121 tttactgttt gttggccttg tgcaactcaa tgatgtggag tcattgaaaa tgattgagcg
    24181 cagcagtgag ctaacgcaac gcctgaaatg atgcataaag cagcgactgg attgagattt
    24241 tcctgaatta gtgagctgat ccgcagcaat attttgttta tcctgtattt tcagagggaa
    24301 tggagtgtaa cgctctgtat taacaaggag agcattaaaa tgggtaaact cacgggcaag
    24361 acagcactga ttacgggcgc attgcaggga attggcgaag gaattgccag aacttttgcg
    24421 cgtcatggcg caaacctaat cttgctggat atctcccctg agatcgaaaa gctggcggac
    24481 gaactgtgtg gtcgtggtca tcgctgtacg gcggttgtcg ccgatgtgcg tgacccggcg
    24541 tcggtcgccg cagctatcaa gcgcgcgaag gaaaaagaag ggcgcattga tatcctggtg
    24601 aataacgcag gcgtttgtcg tctgggcagt ttcctcgata tgagcgatga agatcgcgat
    24661 ttccatattg atatcaatat taaaggcgta tggaacgtca cgaaggcggt gctgccggag
    24721 atgattgccc gcaaagatgg tcgcattgtg atgatgtctt cagtcactgg cgatatggtg
    24781 gccgatccgg gtgaaacggc gtatgcctta acgaaagcgg cgattgttgg cctgactaaa
    24841 tcgctggcgg tggagtacgc gcagtctggt attcgcgtta acgccatctg cccgggatac
    24901 gtacgcacgc caatggcgga aagcattgcc cgccagtcga acccggaaga tccagaatcg
    24961 gtgctgactg aaatggcaaa agcaatcccg atgcgtcgcc tcgccgatcc gctggaagtc
    25021 ggcgaactgg cagccttcct cgcatcggat gaatcaagtt atttaaccgg tacacagaat
    25081 gtgattgatg gcggcagcac actgccggag acggttagcg tcggcatctg attcacctct
    25141 gtttcctccc tgcatttgtg gggaggattt cgtcttgaac ccggttctta ttcccttttc
    25201 aacttccaaa tcaccaaacg gtatataaaa ccgttactcc tttcacgtcc gttataaata
    25261 tgatggctat tagaaagtca ttaaatttat aagggtgcgc aatggccgtt aacttactga
    25321 aaaagaactc actcgcgctg gtcgcttctc tgctgctggc gggccatgta caggcaacgg
    25381 aactgctgaa cagttcttat gacgtctccc gcgagctgtt tgccgccctg aatccgccgt
    25441 ttgagcaaca atgggcaaaa gataacggcg gcgacaaact gacgataaaa caatctcatg
    25501 cagggtcatc aaaacaggcg ctggcgattt tgcagggctt aaaagcagac gttgtcactt
    25561 ataaccaggt gaccgacgta caaatcctgc acgataaagg caagctgatc ccggccgact
    25621 ggcagtcgcg cctgccgaat aatagctcgc cgttctactc caccatgggc ttcctggtgc
    25681 gtaagggcaa cccgaagaat atccacgact ggaacgacct cgtgcgctcc gacgtgaagc
    25741 tgattttccc gaacccaaaa acctccggta acgcgcgtta tacctatctg gcggcatggg
    25801 gcgcagcgga taaagctgac ggtggcgaca aagccaaaac cgaacagttt atgacccagt
    25861 tcctgaaaaa cgttgaagtg ttcgatactg gcggtcgtgg cgcgaccacc acttttgccg
    25921 agcgcggcct gggcgatgtg ctgatcagct tcgagtcgga agtgaacaac atccgcaaac
    25981 agtatgaagc gcagggcttt gaagtggtga ttccgaaaac caacattctg gcggaattcc
    26041 cggtggcgtg ggtcgataaa aacgtgcagg ccaacggtac ggaaaaagca gcaaaagcct
    26101 acctgaactg gctctacagc ccgcaggcgc agaccatcat caccgactat tactaccgcg
    26161 taaataaccc ggaagtcatg gacaaactga aagacaaatt cccgcagacc gaactgttcc
    26221 gcgtggaaga caaatttggc tcctggccgg aagtgatgaa aacccacttc accagcggcg
    26281 gcgagttaga caagctgtta gcggcggggc gtaattaatg tttgcagttt catcaagacg
    26341 cgtgctgccg ggctttacct taagcctcgg caccagtctg ctgtttgtgt gcctgatttt
    26401 gctgctgccg ctctccgcgc tggtgatgca gctggctcag atgagctggg cgcagtactg
    26461 ggaggtgatc accaacccgc aggtggtcgc ggcctacaaa gtgacgctgc tgtcagcgtt
    26521 tgtggcatca atttttaacg gcgttttcgg tttgctgatg gcgtggatct taacccgcta
    26581 tcgctttccg ggccgcacgc tgcttgatgc gctgatggat ttaccctttg cgctgccaac
    26641 ggcagtcgcc ggtttgacgc tggcctcgct cttttccgtg aacggttttt acggtgaatg
    26701 gctggcgaag tttgatatca aagttaccta tacctggctg gggattgcgg tggcgatggc
    26761 ttttaccagt attccgtttg tggtgcgtac cgtgcagccg gtgctggaag agttaggccc
    26821 ggaatatgaa gaagcggcag aaacgctcgg cgcaacgcgc tggcagagtt tctgcaaagt
    26881 ggtgctgccg gagctttctc cggcgctggt ggcgggcgtg gcgctgtcgt ttacccgcag
    26941 cctcggtgag tttggcgcgg taatttttat cgccgggaac attgcctgga agacggaagt
    27001 gacgtcgctg atgatttttg ttcgcttaca ggagtttgat tacccggcgg cgagcgcgat
    27061 tgcttcggtg atcctcgcgg catccctgct gctgctgttc tcaattaaca ctctgcaaag
    27121 tcgctttggt cggcgtgtgg taggtcatta atggcggaag ttacccaatt gaagcgttat
    27181 gacgcgcgcc cgattaactg gggcaaatgg tttctgattg gcatcgggat gctggtttcg
    27241 gcgttcatcc tgctggtgcc gatgatttac atcttcgtgc aggcattcag caaagggctg
    27301 atgccagttt tacagaatct ggccgatccg gacatgctgc acgccatctg gctgacggtg
    27361 atgattgcgc tgattgccgt gccggtgaac ctggtgttcg gcattctgct ggcctggctg
    27421 gtgacgcgct ttaacttccc cggacgccag ttgctgctga cgctgctgga cattccgttt
    27481 gccgtgtcgc cggtggttgc cggtctggtg tatctgctgt tttacggctc taacggcccg
    27541 ctcggtggct ggctggatga acataatctg caaattatgt tctcctggcc gggaatggtg
    27601 ctggtcacca tcttcgtgac gtgtccgttt gtggtgcgtg agctggtgcc ggtgatgtta
    27661 agccagggca gccaggaaga cgaagcggcg attttgcttg gcgcgtctgg ctggcagatg
    27721 tttcgccgcg tcacgctgcc gaacatccgc tgggcgctgc tttatggcgt ggtgctgacc
    27781 aacgcccgcg cgattggcga gtttggtgcg gtgtcggtgg tttccggctc gattcgcggc
    27841 gaaacacttt cgctgccgtt acagattgaa ttgctggagc aagactacaa caccgtcggc
    27901 tcctttaccg ctgccgcgtt gttgacgctg atggccatta tcaccctgtt tttaaagagt
    27961 atgttgcagt ggcgtctgga gaatcaggaa aaacgcgcgc agcaggagga acatcatgag
    28021 cattgagatt gccaatatta agaagtcttt tggtcgcacc caggtgctga acgatatctc
    28081 gctggatatt ccttctggtc agatggtcgc gttgctgggg ccgtccggtt ccgggaaaac
    28141 cacgctgctg cggattatcg ccgggctgga gcatcaaacc agtgggcata ttcgcttcca
    28201 tggcactgac gtcagccgtc tgcacgcgcg ggaccgtaaa gtcggtttcg tgttccagca
    28261 ttacgcgctg ttccgccata tgacggtgtt cgacaatatc gcttttggcc tgacggtgct
    28321 gccgcgtcgc gagcgcccga atgccgcagc catcaaagcg aaagtgacaa aattgctgga
    28381 gatggtccag cttgcgcatc tggcggatcg ttatccggcg cagctttccg gcgggcagaa
    28441 acagcgtgtg gcgctggcgc gtgcgcttgc tgtagaaccg caaattctgc tgcttgatga
    28501 accgtttggc gcgctggatg cgcaggtgcg taaagagctg cgtcgctggt tgcgtcaact
    28561 gcatgaagaa ctgaaattta ccagcgtatt cgtgacccat gaccaggaag aagcgaccga
    28621 agtagccgat cgtgtagtgg tgatgagcca gggcaatatc gagcaggctg atgcgccgga
    28681 tcaggtatgg cgcgaaccag cgacccgctt tgtgctcgaa tttatgggcg aagtgaaccg
    28741 cctgcaggga accattcgcg gcgggcagtt ccatgttggc gcacatcgct ggccgctggg
    28801 ctatacacct gcgtatcagg ggccggtgga tctcttcctg cgcccgtggg aagtggatat
    28861 cagccgccgt accagcctcg attcgccgct gccggtacag gtactggaag ccagcccgaa
    28921 aggccactac acccaattag tcgtgcagcc gctggggtgg tacaacgaag cgctgacggt
    28981 cgtgatgcac ggcgacgacg ccccgcaacg tggcgagcgt ttattcgttg gtctgcaaca
    29041 tgcgcggctg tataacggcg acgagcgtat tgaaacccgc gatgaggaac ttgctctcgc
    29101 acaaagcgcc tgatacgttg agtgaatgtt aaacgcccgg aggcgcttcc cgcgatccgg
    29161 gctttttaat ggcaaggttt gtaacctgta ggcctgataa gacgcgcaag cgtcgcatca
    29221 ggcaacacca cgtatggata gagatcgtga gtacattaga acaaacaatt ggcaatacgc
    29281 ctctggtgaa gttgcagcga atggggccga ataacggcag tgaagtctgg ttgaaactgg
    29341 aaggcaataa cccggcaggt tcggtgaaag atcgtgcggc gctttcgatg atcgtcgagg
    29401 cggaaaagcg cggggaaatt aaaccgggtg atgtcttaat cgaagccacc agtggtaaca
    29461 ccggcattgc gctggcaatg attgccgcgc ttaaaggcta tcgcatgaaa ttgctgatgc
    29521 ccgacaacat gagccaggaa cgccgtgcgg cgatgctggc atacggtgcg gaactgattc
    29581 ttgtcaccaa agagcagggc atggaaggtg cgcgcgatct ggcgctggag atggcgaatc
    29641 gtggtgaagg aaagctgctc gatcagttca ataatcccga taacccttac gcccattaca
    29701 ccaccaccgg gccggaaatc tggcagcaaa ccggcgggcg cattacccat tttgtctcca
    29761 gcatgggaac aaccggcacc atcaccggcg tgtcacgctt tatgcgcgaa caatccaaac
    29821 cggtgaccat tgtcggcctg caaccggaag agggcagtag cattcccggc attcgccgct
    29881 ggcctgcgga atatctgccg gggattttca acgcttcgct ggtggatgag gtgctggata
    29941 ttcatcagag cgatgcggaa aacaccatgc gtgaactggc ggtgcgggaa ggaatattct
    30001 gtggcgttag ctccggcggc gcggttgccg gagcactgcg cgtggcaaaa gctaaccccg
    30061 gcgcggtggt tgtggcgatc atctgcgatc gcggcgatcg ctacctctcc accggcgtgt
    30121 ttggggaaga acactttagc cagggggcgg ggatttaaga ttattgcgca ggcactgttt
    30181 tatccgtctg cgcaattagc gcatgtaaaa aaggttgtgc gttctcatcg cttttaccgg
    30241 gcatcttcac gatatacggc ttcccggtaa tcgatgacga cgaggccacc ttatcaataa
    30301 actgctcggc agtgtcaatg cggttacggg tattgccgag cttcagacgc aggtgggaaa
    30361 ccgcttcatc gcaggtatgt tcatcaccgt tgcgcacaaa tatcaaatcc tttttctgtg
    30421 ctaatccctc cagcatggcg ttgatgcggg cttcttcatg aacagttaac ttcgcgtaag
    30481 cggggagtgt cattagcagc gtaatggcca gacaaatgat tttcttcact gaattacctt
    30541 atccttgttt tgtaatagcc gatttgactg ccagaatcag gtaaagtttc ggttggaata
    30601 cgatgaggat catcggcaag gagaaaaaat taaatgagta gtttgttgtt gtttaacgat
    30661 aagagcaggg cattgcaggc ggatatcgtc gccgtgcagt cgcaggtagt gtacggcagc
    30721 gtaggcaaca gcattgccgt gcctgctatc aaacagaacg gcctgaatgt ctttgccgtg
    30781 ccgacggtat tgctgagcaa tacaccgcat tatgacactt tctacggtgg tgcgattccg
    30841 gacgaatggt ttagcggcta tttgcgtgcg cttcaggagc gtgatgcgtt gcgccaactt
    30901 cgtgctgtaa ccacgggcta tatgggaacg gcatcgcaaa tcaaaatcct tgccgagtgg
    30961 ctgactgcgc tacgcaaaga tcatcctaac ctattgatca tggtcgatcc agtgattggc
    31021 gatattgata gcggaattta tgtcaaacct gaccttcccg aggcgtatcg acaatattta
    31081 ctgccgctgg cgcagggaat tacccccaat atttttgagc tggaaatcct gaccggtaaa
    31141 aattgccgcg atctcgacag tgctattgct gccgcaaaaa gtctgctttc agacacatta
    31201 aaatgggtgg tgattaccag cgcctccggt aatgaagaaa atcaggagat gcaggtggtg
    31261 gtggtcagtg ccgacagcgt gaatgtcatt tcccattcac gggttaaaac tgacctgaaa
    31321 gggactggcg acctgttttg tgctcagctt atcagtggct tgttgaaagg taaggaatta
    31381 actgacgcgg tacatagcgc gggattacgc gtactggaag tgatgcgcta cacccagcag
    31441 catgagagcg atgaattgat tttgccgccg ctggcggaag cataaaaaat ggcgccgatg
    31501 ggcgccattt ttcactgcgg caagaattac ttcttgatgc ggataaccgg ggtttcaccc
    31561 acggttacgc taccggacag tttgatcagt tctttgattt cgtccatgtt ggagataaca
    31621 accggagtca gggtagactt ggctttctct tccagcagcg gcagatcaaa ttcaatgaca
    31681 gtatcgccaa ctttcacgcg ctgaccttct tcagcaatac gcttgaagcc ttcgcctttc
    31741 agttcaacgg tgtcgatacc gaagtggacg aacagttcaa cgccgctatc agattcgata
    31801 gagaacgcgt ggttggtttc aaagatttta ccaatggtgc cgtctacagg cgcgaccatt
    31861 ttgtttccag ttggtttgat agcaatgcca tcaccaacga ttttttccgc aaaaacgaca
    31921 tccggcacgt cttcgatatt gacgatctcg ccagagagcg gagcaatgat ctcaatagtt
    31981 ccggtatcct tcttgtcgtc ggaaaccaga gatttcagtt tatcgaacaa acccatgatc
    32041 ttctcctaag cagtaaattg ggccgcatct cgtggattag cagattgttt tttcttcaat
    32101 gaacttgtta accagcgtca ttaactcgtc cgttgtcggt tgagcaagag cctgctctgc
    32161 taacaccttc gcatcttcga agttcgtgtt acggataatc ttcttaatgc gcgggataga
    32221 aatggcgctc atagagaatt cgtccagacc catccccagc aacagaagtg tagcacgttc
    32281 atcgccagca agctcaccac acatgccagt ccatttgcct tcagcatgag aagcatcaat
    32341 aacttgcttg atcaagttca gcacggacgg tgacattggc tggtaaaggt gtgaaatcat
    32401 atcattacca cggtcaactg ccagagtgta ctgcgttaaa tcattggtgc cgatactaaa
    32461 gaaatcaact tctttggcta aatgacgtgc aattgttgcg gcagccggtg tttccaccat
    32521 tacgccgatt tcaattgact cgtcaaacgc tttaccttcg tcgcgcagtt cctgtttgta
    32581 gatttcgatc tctttgcgca gtgcacgcac ttcttcaaca gagatgatca tcgggaacat
    32641 aatgcgcaat ttaccgaaag ccgaggcacg caggatagcg cggagctgat cgcgcaggat
    32701 ctctttacga tccatcgcga tacggatagc gcgccagccg aggaacgggt tctcttcttt
    32761 cgggaagttc atgtatggca gctctttgtc gccgccgatg tccatggtac gaacgataac
    32821 cgcctgcgag ccacacgctt cagccactgc tttgtaagca gcaaactgtt cttcttcagt
    32881 gggcagcgcg tcgcggtcca tgaacagaaa ctcagtacga tacagaccaa cgccttcagc
    32941 gccgttacgc tctgcacctt caacgtcacg aaccgtacca atgttagcgc acacttctac
    33001 ctggtgaccg tccagcgtaa tagctggcag atctttcagt ttagcaagct ctgctttttc
    33061 agaagccact tgctcctgaa cagcgcgcat tttatcaata acttcgttgg ttggattgac
    33121 gtaaacctga ttatttacgg catccagaat cagatagtcg tcatttttca cctgagaggt
    33181 gacgctaccg gtacccacga tagcaggcag ttccagagaa cgcgccatga tagaggtgtg
    33241 ggaagtacgg ccacccgcgt cggtgatgaa acccagcacc ttcttcaggt tcagctgtgc
    33301 ggtttcggac ggcgtcaggt cagcggcaac cagaatgact tcatcctgaa tggcgctcag
    33361 gtcgataatc ttcaggccca ggatgttgcg cagaaggcgc ttaccgatat cacgtacgtc
    33421 agccgcacgt tctttcaggt attcatcatc cagctcttcc agggcagaag cctgaccttc
    33481 gataacttca tgagcagctg cgtcagctgt catgtgctta tctttaatca gggctatgat
    33541 ttcctgctcc agctcctcat cttcgagcag cataatatgc ccttcaaaga tggcttcttt
    33601 ttcttcaccg aacgtttcac cagctttcgt tttgatcgtt tccagctggg ctgatgcctt
    33661 ggcacgaccg ctcagaaaac gttcaacttc ctgatcaacc tggtcggcag aaattttttt
    33721 ccggtcaatg acaatttcgt cttctttcag aagcagagct ttaccgaaag cgatacccgg
    33781 ggatgctaaa atgcctgaaa tcataaccct accttacttg tgactgattt ttaaaagaac
    33841 ccgggaaatt actcgagttc cgccatcagt ttaaccagat gttcaaccgc tttctgctcg
    33901 tcttcgcctt ctgcggagat agtcacaacg gtaccttgag tcaggcccag agtctgcagt
    33961 ttaaacaggc ttttcgcgct ggcgcttttg ccgttggaag tcacagtaat ttcagaagtg
    34021 aagcccttag cttcttttac aaactgggca gcagggcggg tgtgcagacc gttcggagcg
    34081 gtaatggtaa cttcttgctg gaacattgta tttccccaac ttataggttt agtgttgtgg
    34141 aactaaagtc tagcctggcg gttcgacttt agcctgtatt gttagcgcca gtttttaaca
    34201 gacgcgacgc acgaagagcg caatacagtc cattgctggc gggtgttgac cgctgacgtt
    34261 tcgtacgtca ttaaacatta tgccgctaaa ggaagaattg aaccaaatca taaaatcgat
    34321 tcagctttgg cttttcctgt aacgattaat ttcgcgcatc aaaataatta gtctggttaa
    34381 ataccagttc cgacaggctg aatcaatgcc aggaggggcg aaagtttgaa gcaggccaca
    34441 aaaaagcacc tgaaaaggtg cttttttacg catttttaac aagctggcat tactgttgca
    34501 attctttctc agtgaagaga tcggcaaaca atgcggtgct taaataacgc tcacccgatg
    34561 atggtagaat aaccacaata ttcttgttgg taaagctttc atcttcttgt agtttcaacg
    34621 ccgcggcaac agctgctcca gaagagatac ctgcaagaat accttcttct tccatcagac
    34681 gacgggcggt agaaatggct tcttcattgg tgatgccaat gactttatcg accagcttga
    34741 gatcgaggtt agccgggata aaacctgcgc caataccctg aattttatgc gggccaggtt
    34801 taatctcttc accagccagt gcctgggcga taactggaga atcggttggc tcaacggcga
    34861 cagagataag atcggtcttg cctttggtgc ctttaatgta gcggctgacg ccggtcagcg
    34921 taccgccagt gccaacgcca gcaataaata catcaacctg accgtcggta tcttcccaga
    34981 tctccggacc ggtggttttt tcgtggattt cagggtttgc cggattgctg aattgttgca
    35041 gcagcaggta tttctctgga ttgctggcga caatttcttc tgctttttgg attgcgcctt
    35101 tcatgccttt agcaccttcg gtcagcacca ggtttgcacc taacgctttc agcagcttgc
    35161 ggcgttcaat actcatggtt tctggcatgg tcagggtaag tttgtaaccg cgagcggcag
    35221 cgacataggc cagtgcaatc ccggtattac cgctggttgg ttcaaccagt tcaacgcctg
    35281 gtttcagcac gccgcgcttt tcggcatccc aaatcatgtt agcaccgata cggcacttaa
    35341 cgctgaagct ggggttacga gattccacct tcgccagaat gcgtccgtta ccgatgcgat
    35401 tcaggcgaac cagcggcgtg tgaccgatag tcagcgagtt atcttcaaaa atcttactca
    35461 tggcctgtcc ttaactgtat gaaattggga tacaacaggt agcatacccg ctcagagaat
    35521 atgcggaagt aaggatttag catatctata tgcagaaggg aaataatgac atgcaagatg
    35581 gaataagggg cggcataagc cacccctgtt tcacacaagc ggtttacaaa ataaccggta
    35641 gattgttacc gccacatcgc gtgtttatcg cgatagcaat cgacccacat cgccgtcgca
    35701 ccacaaacgg caacgggcat gatgaacaga ttaagcagag ggatcatcgt aaacaggctg
    35761 gttaaagcac caaactgcat attggtgatt ttgcgtgtgc gcagggcggt gcgcatctct
    35821 ttaaacggca ctttgtggtt atcgaagggg taatcgcaat actggatggc taacatccag
    35881 gcgctaaaca ggaaccaaag taccggcgcg acggtttgcc caataccagg gatgaagtaa
    35941 agaattagca atacaattgc gcgcggcaga taccaggcaa atttttgcca ttcgcgtttc
    36001 atgattcgcg gcatatcttt catgataccg aaaatcccgg tatctggcgg tgtagcgcca
    36061 gtcaatcgtg cttccagttg ttcagccaat aaaccattga acggagcggc aatccagtta
    36121 gcaatcgttg agaagaaata gccaaacact aacagcacag agatgacagc cagaggccac
    36181 aacagatagc tcagccattg taaccagtcc ggaacataac tcatgagagt cgggatccag
    36241 acatcgagtt gcgtaaagag ccaccagaat gcgcccccca tcaacaaaat attgaccagc
    36301 agcggtaaaa taacgaaacg ccgaatccca ggttgcgaga cgagcttcca gccttgcgca
    36361 aaatagtaaa aaccgctgcg tggggcagat gtgaatgatg aaaccataat caggatgagc
    36421 tccttttgac caatcccagg aaaattctgc gtattttacc gggtaattgc gcaatggaca
    36481 gttaggatat gttcgaaaaa acagcaaaaa gcacgatttc atctatcttt gtgctgtgaa
    36541 agttaatagt gcacttgcac ttgaggtaat cggcaaatac tcttagtgag taaatgtttg
    36601 ccgtggtggc aaggtgttag aacaacagag aatataatga tgcaggattt gcgtctgata
    36661 ttaatcattg ttggcgcgat cgccataatc gctttactgg tacatggttt ctggaccagc
    36721 cgtaaagaac gatcttctat gttccgcgat cggccattaa aacgaatgaa gtcaaaacgt
    36781 gacgacgatt cttatgacga ggatgtcgaa gatgatgagg gcgttggtga ggttcgtgtt
    36841 caccgcgtga atcatgcccc ggctaacgcc caggagcatg aggctgctcg tccgtcgccg
    36901 caacaccagt accaaccgcc ttatgcgtct gcgcagccgc gtcaaccggt ccagcagccg
    36961 cctgaagcgc aggtaccgcc gcaacatgct ccgcgtccag cgcagccggt gcagcaaccc
    37021 gtgcagcagc ctgcctatca gccgcagcct gaacagccgt tgcagcagcc agtttcgcca
    37081 caagtcgcgt cagcgccgca gccagtgcat tcagcaccgc aaccggcaca acaggctttc
    37141 cagcctgcag aacccgtagc ggcaccacag cctgagcctg tagcggaacc ggctccagtt
    37201 atggataaac cgaagcgcaa agaagcggtg attatcatga acgtcgcggc gcatcacggt
    37261 agcgagctaa acggtgaact gcttcttaac agcattcaac aagcgggctt catttttggc
    37321 gatatgaata tttaccatcg tcatcttagc ccggatggca gcggcccggc gttattcagc
    37381 ctggcgaata tggtgaaacc gggaaccttt gatcctgaaa tgaaggattt cactactccg
    37441 ggtgtcacta tctttatgca ggtaccgtct tacggtgacg agctgcagaa cttcaagctg
    37501 atgctgcaat ctgcgcagca tattgccgat gaagtgggcg gtgtagtgct tgacgatcag
    37561 cgccgtatga tgactccgca gaaattgcgc gagtaccagg acatcatccg cgaagtcaaa
    37621 gacgccaacg cctgatacac ttaaggcaaa ttaactcctc ttcgaacccc cgcttgtcgg
    37681 gggtttttag cattgatggt gcgatatgga atcaatcgaa caacaactga cagaactgcg
    37741 aacgacgctt cgccatcatg aatatcttta tcatgtgatg gatgcgccgg aaattcccga
    37801 cgctgaatac gacaggctga tgcgcgaact gcgcgagctg gaaaccaaac atccagaact
    37861 gattacgcct gattcgccta cccaacgtgt aggtgctgcg ccgctggcgg ctttcagcca
    37921 gatacgccat gaagtaccaa tgttgtcgct ggataacgtt tttgatgaag aaagctttct
    37981 tgctttcaac aaacgtgtgc aggaccgtct gaaaagcaac gagaaagtca cctggtgctg
    38041 tgagctgaag ctggatggtc ttgccgtcag tattctgtat gaaaatggcg ttttagtcag
    38101 tgccgcgacg cgtggcgatg gcactaccgg ggaagatatt acgtctaatg tgcgtactat
    38161 tcgcgccatt ccgctgaagc tacacggaga gaatatcccg gcgcgtctgg aagtgcgtgg
    38221 tgaagtgttc ctgccgcagg cgggcttcga aaagattaac gaagatgcgc gacgcacggg
    38281 cgggaaagtg tttgctaacc cacgtaatgc ggctgctggt tcactgcgtc agcttgatcc
    38341 gcgtattaca gcgaagcgac cgctcacttt cttctgctat ggcgtcggtg ttctggaagg
    38401 tggcgagctg ccggatactc atcttggccg tttactgcaa tttaaaaagt ggggtttgcc
    38461 ggtcagcgat cgggtaacgc tttgtgaatc ggcggaagaa gtgctggcgt tctaccacaa
    38521 agtagaagaa gaccgcccga cgctgggctt tgatatcgac ggcgtggtga ttaaggtcaa
    38581 ctcactggca cagcaggagc agcttggctt tgtcgcgcgt gccccgcgct gggcggtagc
    38641 gtttaaattc ccggcgcagg aacagatgac ctttgtgcgt gacgtcgagt ttcaggttgg
    38701 gcgtactggc gcgattacgc ctgttgcgcg tctggaacct gtccatgttg ctggcgtgct
    38761 ggtgagtaac gccacactgc ataatgctga cgaaatcgaa cgtcttggtt tacgcattgg
    38821 cgataaagtg gtgattcgcc gtgcaggcga cgtgatcccg caggtggtga acgtcgtgct
    38881 ttctgaacgc ccggaagata cccgtgaggt tgtattcccg acgcattgtc cggtatgtgg
    38941 ttctgacgtt gagcgtgtgg aaggtgaagc ggttgcccgc tgtaccggtg gcctgatttg
    39001 cggtgcgcag cgtaaagagt cgctgaaaca ctttgtttcc cgccgtgcga tggatgttga
    39061 cggaatgggc gacaaaatca tcgatcagct ggttgaaaaa gaatatgtcc acactccggc
    39121 ggatctgttc aaactcaccg caggcaaact taccggactg gagcgtatgg ggccaaaatc
    39181 ggcacaaaac gtggttaacg cgctggaaaa agcgaaagaa accacctttg ctcgcttcct
    39241 ctatgcactt ggcatccgtg aagtcggcga ggccaccgca gcaggtctgg cggcatattt
    39301 cggcacgctg gaagcgctgg aagccgcttc gattgaagag ctgcaaaagg tgcctgatgt
    39361 tggcattgtc gttgcctccc acgttcacaa cttctttgcc gaagaaagca accgcaatgt
    39421 catcagcgag ctgttggcgg aaggcgttca ctggcctgcg ccggtggtga ttaacgcgga
    39481 agagattgac agcccgtttg ctggtaaaac cgtggtgctt acgggcagct taagccagat
    39541 gtcgcgtgat gacgctaaag ctcgactggt cgaactgggc gcgaaagtcg cgggcagcgt
    39601 gtcgaagaaa accgatctgg tgatagcggg tgaagctgca ggatctaaac tggcgaaggc
    39661 gcaggaactg ggtattgaag tcatcgacga aacggaaatg ctgcgtttgc tgggtagctg
    39721 agatggaaaa agagcagctg attgaaatag ccaatacgat aatgccgttt ggcaagtaca
    39781 aagggcgtcg cttaatcgac ctgccggagg agtatctgct gtggtttgcc cgcaaagatg
    39841 agttcccggc agggaagctc ggtgagctaa tgcaaatcac gctgctgatt aaaaccgagg
    39901 ggctgacgca actggtccag cccctgaagc gtccgcttta agctttatcg gcgctgcttt
    39961 cctgctgcgc ctgtaactgt tcggtctggc gtttgtatcg acgcgccagc accgcacaga
    40021 ccatcaattg gatctgatgg aaaatcatca ggggcagcac catcataccg atcaccgatg
    40081 tggggaacag aatgtttgcc atcgggatgc catttgccag actctttttc gaaccacaaa
    40141 agacgatagt aatttcatct gccttattga agcccagtcg gcgtgccata aagacgttaa
    40201 ctacaatcac gatagccaga agaacgcagc tgaccaccac gataaacagc aatgatcccc
    40261 agccaacttt gtgccagata ccgttaacga cggcttcgct gaatgccgta taaaccacca
    40321 acagaatgga cgtctggtca gttttcgcaa tccatttttt attacgcgac acccagtcac
    40381 caatccacgg ccgggaaaga tgccccaaca caaatggcag caacagttgc agcataattt
    40441 taccgacctg ctcaaggctg ccccctgcac cgtgaacatt catcaccaga ccaaccagca
    40501 atggtgaaag gaaaatcccc agcaggctgg atgctgacgc agaacaaacc gccgccgcaa
    40561 cgttaccgcc cgccattgac gtgaaggcga ttgcagactg cacggtcgcg gggagaatgc
    40621 acaagtagag aaaaccggag tagagcatcg ggtcgacatt taccggtttc caccaggcaa
    40681 acagcacgcc cagaatcgga aacagcacga aggtgctgca cattacccac aaatgcagtc
    40741 gccagtggcc accgccagca ataatcgcct cacgcgacaa cttcgcgccg tgcataaaaa
    40801 acagcagagc aatcgctgca gtggtcagat tttcaaagaa ggggacgaaa tcgcctctgg
    40861 ccggaaagaa agaggccagc aacaccactg tgatcagggt taaggtgaaa ggatcgagga
    40921 tacgaaaaag tttcataaag actccagatg atcgatggtt ctattgtgct tcttccagtt
    40981 tgagaaataa aattgattta ttgcatccat tcatgaaaaa aacagatgaa ttattcttta
    41041 aagcaattaa aggttttcgt cacagtagcg caggagaaaa gttttagtcg tgcaggagag
    41101 cgtatcggcc tgagccagtc ggcagtgagt cacagtgtga aggagctgga aaatcatact
    41161 ggtgttcgcc tgctggacag aaccacgcgt gaagtggtgc ttacagatgc agggcagcag
    41221 ttggctttgc gtcttgagcg actgctggat gaacttaaca gcacgttgcg cgataccggg
    41281 cgtatggggc aacaactgag cggaaaagtt cgggtcgctg ccagccagac catttccgcg
    41341 catcttattc cgcaatgcat tgctgaaagc catcgccgct atcccgatat tcagtttgtc
    41401 ctgcacgatc gtccgcagca gtgggtgatg gaaagtattc gccagggaga tgtcgatttt
    41461 ggcatcgtca tcgatccagg cccagtgagc gatctgcaat gtgaagcgat tctttccgag
    41521 cctttcttct tgctttgcca tcgcgatagc gctttagccg cggaagatta tgtaccctgg
    41581 caggcattac agggagcgaa attggtgttg caggattacg cgtcaggcag ccgaccgctg
    41641 attgacgcag ctctggcgcg caacggtatt caggcgaata ttgtgcagga gattggtcat
    41701 ccggcgacgc tgttcccgat ggtagccgca ggcatcggca ttagtattct tcccgcactg
    41761 gcgctgccgt tacctgaagg tagcccgctg gtggtaaagc gcattacgcc ggtggtggaa
    41821 cgccagttga tgctggtgcg caggaagaac cgttcacttt ccaccgccgc acaagcgtta
    41881 tgggaagtgg tacgaggcca gggccgtgcg ctaatggctg ggcgggaagg ggatccgctg
    41941 tatcagatat agagctattc atactgccgg gagccccggc agtaatcaat cagaaattaa
    42001 atttgatgga gtagattatt gcatcctggt aaaaatcttc gcccagtttg tcatccgcat
    42061 aacgatactg aattcctgtg gtgatgtgtg aagttgcatt ccaccaaagt gcgacggcac
    42121 cgttcagacc ttctttgccg ccattacctg ccgcgtaggt ggcatcacga tcaaactcgt
    42181 actcgttcca gttggtgagg gtgaatttct cactgcccag cataaagctg taacctgcaa
    42241 cccaaccggc gacataaccg ttatcgccgg tataataggt ttggtctgta tagcgttttg
    42301 caaagaacgg tttgaaccac caaccgctgc cggtgaaatt gtagccgatc ccgtaaagga
    42361 acatatcgtc atggaaattc acgcgattcg cagaaccgta ggtgccatac gcatgcaagt
    42421 agagattaaa tccggtgtca cccaggtaaa tacggttggt atttttaaag gtataacgct
    42481 gctcgctacc cggtttatca tgacgatcat tataaaagtt ttcccagtcg aagaacccgt
    42541 acatttctcc ccaactaaag ttagcgccgc cttccagttc cagatatcca aaatcatctt
    42601 tgtgtgattt ggtcgacgat ttctcagtgg ttctgctagt ccagtcaaga taatgaatac
    42661 ctatatcagc aaaaccgcct ttaaattctg catgggatac tgctggtatc gccaatacag
    42721 aagagagtgt cagagtaaaa agatgttttt tcatagcata attccctatg ccgatcgtta
    42781 ttagttgatt gcttaataaa aaggttgatt aaaagcggca tattgttaat gcaagggaaa
    42841 ggagagtgcc aata
//
LOCUS       DAPHCV010000026        42763 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.026, whole genome shotgun sequence.
ACCESSION   DAPHCV010000026 DAPHCV010000000
VERSION     DAPHCV010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.026"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..2024)
                     /locus_tag="RH033_001465"
     CDS             complement(<1..2024)
                     /locus_tag="RH033_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015987600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HDV2990675.1"
                     /translation="MGFSSARNLGRDISAGFSPPRRMPISEAVKKFMRVPKGAGNSVP
                     WDPELTPYIIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWIVYTIVCDPSDMLVVQ
                     MTEDKAREHSKKRLDRTFRSSAAVKKRMSPRRNDNNVHDKTFRDGSFLKIGWPSVNIM
                     SSSDYRFVALTDYDRFPENIDSEGDGFSLASKRTTTFMSAGMTLVESSPGRDICDSKW
                     RRKSPHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPAMDAMTGYRNEPDPFKASE
                     AAYLLCPHCSGIITAEKKRELNSAGVWLREGQVIDRNGNVSGEPRRSRIASFWMEGPA
                     AAYQTWAQLVYKLLTAEQEYEATGSEETLRAVINTDWGLPYLPRANMEQRKSELLEQR
                     AEPVPSRSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRSD
                     CDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDNA
                     YKFWRRCRRDGLGKRIYLFKGDSIRRAKLISRTFPDNTGRTGRRAQAAGDVPLWLLQT
                     DALKDRVNNALWRNSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAFD
                     LMVYAEALVILHGYEKIRWPDAPEWASRETWLECVPDSTEPSPSPEPVSTP"
     gene            complement(2033..2572)
                     /locus_tag="RH033_001466"
     CDS             complement(2033..2572)
                     /locus_tag="RH033_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HDV2990676.1"
                     /translation="MELLMSNVSGIGDAYYWSVFKIAEAFGLHRDTVKKRLLAANTPV
                     AATVRGNPVYALQHVGPALFSVKHEAADSVHDPSRMEPKERKDWYQSENERIKLEKEQ
                     RKLIPVDEVVIVYSSMRKAVVQVLETIPDVLERDCALTPQAVGVVQQAIDDLRYTLQE
                     KSYEACAAELIPDEEGESL"
     gene            2905..3225
                     /locus_tag="RH033_001467"
     CDS             2905..3225
                     /locus_tag="RH033_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2990677.1"
                     /translation="MSIKVFRSDLQTESESDYRNWLGDNPDGYVVNALKRTRGKGNKS
                     DERFTRIHRASCKTINPLKRKIDKTGFTTGDYQKICSTNFLNVENEAKKMTGLTSIIK
                     CRCI"
     gene            complement(3606..4049)
                     /locus_tag="RH033_001468"
     CDS             complement(3606..4049)
                     /locus_tag="RH033_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HDV2990678.1"
                     /translation="MSRVTAIISALIICIIVCLLWAVNHYRDNAIAYKEQRDKAASTI
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKWLRVKAVCPDMHKITAAS
                     GVDDGTSPRLTDTAQRDYFTLRKRIETSDKMIRGLQQYIRTQCVR"
     gene            complement(4046..4543)
                     /gene="rrrD"
                     /locus_tag="RH033_001469"
     CDS             complement(4046..4543)
                     /gene="rrrD"
                     /locus_tag="RH033_001469"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HDV2990679.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGDDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWIYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(4543..4758)
                     /gene="essD"
                     /locus_tag="RH033_001470"
     CDS             complement(4543..4758)
                     /gene="essD"
                     /locus_tag="RH033_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HDV2990680.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            complement(4826..5878)
                     /locus_tag="RH033_001471"
     CDS             complement(4826..5878)
                     /locus_tag="RH033_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDV2990681.1"
                     /translation="MKNTVKINSVDLINADCLHFIQSLPDDSIDLIVTDPPYFKVKPN
                     GWDNQWKGDEDYLKWLDHCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFNVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERVLFAEHYQGPYRGKSDGYAAKERELKQHIMA
                     PLISYFRDARAELGITAKQIAEATGKKNMVSHWFGASQWQLPNEADYRKLQALFSRIA
                     AEKFQEQQLEQPHHQLVASYDSLNRKYSELLDEFKSLRRYFSVSVSVPYTDVWMHKPV
                     QFYPGKHPCEKPADMLRQIINASSRPGDLVADFFMGSGSTIKAAMALGRRALGVELES
                     ERFNQTVKEINELVGK"
     gene            complement(6029..6232)
                     /locus_tag="RH033_001472"
     CDS             complement(6029..6232)
                     /locus_tag="RH033_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2990682.1"
                     /translation="MLKQQDMTETAAAVLHFLPADKWVTPRMMTRTTGVSEARCQLIL
                     TQLVLAGLAKDNGGYGNKFRRCQ"
     gene            6503..6949
                     /locus_tag="RH033_001473"
     CDS             6503..6949
                     /locus_tag="RH033_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2990683.1"
                     /translation="MNKMTVLLLSATIISGCTSSVPLIKKTQSGKPEGFYQNTTKDKV
                     KDALVNYCNSRGLIIYNADNSSVICGKELEGGSAVFGQMLIGNAYSTTPVSKVKFTIA
                     QVNNDTKVWADMWMETQMAMGQVQQMAITDNASKNTIQQRLDELKP"
     gene            complement(7034..7399)
                     /locus_tag="RH033_001474"
     CDS             complement(7034..7399)
                     /locus_tag="RH033_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HDV2990684.1"
                     /translation="MRDIQMVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVGGMTFMALARKHGRSDCWVGRLLQKA
                     EGVVDGMLMMLEIELEMDL"
     gene            complement(7417..8406)
                     /locus_tag="RH033_001475"
     CDS             complement(7417..8406)
                     /locus_tag="RH033_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDV2990685.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKHRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            complement(8414..9223)
                     /locus_tag="RH033_001476"
     CDS             complement(8414..9223)
                     /locus_tag="RH033_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HDV2990686.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAVASGANARTKEPGKF
                     LSSQQTVELVHELTNTQNLGVDPVSVIHGGNERGTYVCKELVYAYAMWISPSFHLKVI
                     RTFDMVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAHDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYSRT
                     AINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            complement(9243..9632)
                     /locus_tag="RH033_001477"
     CDS             complement(9243..9632)
                     /locus_tag="RH033_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDV2990687.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKTAGAAA"
     gene            complement(9629..9955)
                     /locus_tag="RH033_001478"
     CDS             complement(9629..9955)
                     /locus_tag="RH033_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HDV2990688.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRALLAGEENARLRATHWLH
                     ERDLKV"
     gene            complement(9952..10605)
                     /locus_tag="RH033_001479"
     CDS             complement(9952..10605)
                     /locus_tag="RH033_001479"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HDV2990689.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            complement(10605..11099)
                     /locus_tag="RH033_001480"
     CDS             complement(10605..11099)
                     /locus_tag="RH033_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HDV2990690.1"
                     /translation="MIMSLLNDVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEPEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            complement(11096..12052)
                     /locus_tag="RH033_001481"
     CDS             complement(11096..12052)
                     /locus_tag="RH033_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV2990691.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTVRTAIARLEAEGWLTRKARRQGNRNASNVYQLNVAKLQAAAFSQLS
                     DSDPSKSDASKSDASKSDPSKFDASKSGKKAGFHPSESGGDPSVKSKHDPSDKKPSRP
                     DASQPDTQTAEQDFLTRHPDAVVFSPKKRQWGTQDDLTCAQWLWKKIIALYEQAAECD
                     GEVVRPKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREK
                     WDELSLRLSPSVSTYTEKREDPYFKASYDNVDYSQIPAGFRG"
     gene            complement(12042..12221)
                     /locus_tag="RH033_001482"
     CDS             complement(12042..12221)
                     /locus_tag="RH033_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HDV2990692.1"
                     /translation="MRQVNRWFKDHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            complement(12397..12954)
                     /gene="ymfL"
                     /locus_tag="RH033_001483"
     CDS             complement(12397..12954)
                     /gene="ymfL"
                     /locus_tag="RH033_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HDV2990693.1"
                     /translation="MGKHHWKIEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCISE
                     SNDARECAAPGVVASIASGCGETNA"
     gene            complement(12998..13198)
                     /locus_tag="RH033_001484"
     CDS             complement(12998..13198)
                     /locus_tag="RH033_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV2990694.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            13289..13963
                     /locus_tag="RH033_001485"
     CDS             13289..13963
                     /locus_tag="RH033_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional repressor"
                     /protein_id="HDV2990695.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            complement(14371..14850)
                     /locus_tag="RH033_001486"
     CDS             complement(14371..14850)
                     /locus_tag="RH033_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2990696.1"
                     /translation="MTRDDLAFDIHYSFFLEKMNYTLLNRIDKSITLVLIVLGFSVFA
                     PYSNMFIFGASVAILSVLQLVYQFGQEAGLSKEQMRQYKRLIVEFSSLSDDELRTRFL
                     KVQDADSNPWRSLQDAAFKRTCIALGRVDESEISFMHSVIAWFAGDLPRSKRCNNDK"
     gene            15088..15398
                     /locus_tag="RH033_001487"
                     /pseudo
     CDS             15088..15398
                     /locus_tag="RH033_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15413..15775
                     /locus_tag="RH033_001488"
     CDS             15413..15775
                     /locus_tag="RH033_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2990697.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGR"
     gene            15841..16665
                     /locus_tag="RH033_001489"
     CDS             15841..16665
                     /locus_tag="RH033_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HDV2990698.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDAIQATK
                     AAAAVRKITIEANQTADFEDNDFNGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            16793..17329
                     /gene="yfdR"
                     /locus_tag="RH033_001490"
     CDS             16793..17329
                     /gene="yfdR"
                     /locus_tag="RH033_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HDV2990699.1"
                     /translation="MSFIKTFSGKHFYYDKINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKQMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVIPLAPGHA
                     YGMFMERFNELSELRKCA"
     gene            17320..17682
                     /locus_tag="RH033_001491"
     CDS             17320..17682
                     /locus_tag="RH033_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2990700.1"
                     /translation="MRMNVFEMEGFLRGRCVPRDLKVNETDAEYLVRKFDALEAKCAA
                     QENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
     gene            17682..17987
                     /locus_tag="RH033_001492"
     CDS             17682..17987
                     /locus_tag="RH033_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2990701.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            18214..19377
                     /locus_tag="RH033_001493"
     CDS             18214..19377
                     /locus_tag="RH033_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDV2990702.1"
                     /translation="MSLFRRGEIWYASFTLPNGKRFKQSLGTKDKRQATELHDKLKAE
                     AWRVSKLGEIPDITFEEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESK
                     IYSAMQKMTNRRHEENWRLRAEACRKKGKPVPEYTPKPASVATKATHLSFIKALLRAA
                     EREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSN
                     IINLEWQQIDMQRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKES
                     CTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPNLSQSKNKEGTNDV"
     gene            complement(19392..19467)
                     /locus_tag="RH033_001494"
     tRNA            complement(19392..19467)
                     /locus_tag="RH033_001494"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19432..19434),aa:Thr,seq:cgt)
     gene            complement(19582..20835)
                     /gene="proA"
                     /locus_tag="RH033_001495"
     CDS             complement(19582..20835)
                     /gene="proA"
                     /locus_tag="RH033_001495"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDV2990703.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSESILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCSLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLLALSKQ
                     MAESGVTLHADAGALAQLQTGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(20847..21950)
                     /gene="proB"
                     /locus_tag="RH033_001496"
     CDS             complement(20847..21950)
                     /gene="proB"
                     /locus_tag="RH033_001496"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDV2990704.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            22239..23294
                     /gene="phoE"
                     /locus_tag="RH033_001497"
     CDS             22239..23294
                     /gene="phoE"
                     /locus_tag="RH033_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HDV2990705.1"
                     /translation="MKKSTLALVVMGIVASVSVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPISGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYVDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(23333..23734)
                     /gene="crl"
                     /locus_tag="RH033_001498"
     CDS             complement(23333..23734)
                     /gene="crl"
                     /locus_tag="RH033_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HDV2990706.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVRIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(23792..25036)
                     /gene="frsA"
                     /locus_tag="RH033_001499"
     CDS             complement(23792..25036)
                     /gene="frsA"
                     /locus_tag="RH033_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HDV2990707.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKVVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(25128..25586)
                     /gene="gpt"
                     /locus_tag="RH033_001500"
     CDS             complement(25128..25586)
                     /gene="gpt"
                     /locus_tag="RH033_001500"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDV2990708.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            25847..27304
                     /gene="pepD"
                     /locus_tag="RH033_001501"
     CDS             25847..27304
                     /gene="pepD"
                     /locus_tag="RH033_001501"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HDV2990709.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVDTYQEILKNELAEKEKNLALLLDSVANDKAALTATSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(27361..27975)
                     /locus_tag="RH033_001502"
     CDS             complement(27361..27975)
                     /locus_tag="RH033_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HDV2990710.1"
                     /translation="MILLQLSSAQGPEECCLAVRKALDRLIKEATRQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAALKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(27972..29111)
                     /locus_tag="RH033_001503"
     CDS             complement(27972..29111)
                     /locus_tag="RH033_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HDV2990711.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRNSLGSIGGGNHFVELQQIDQIIDAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALRYIAEHDDALAFARINRQL
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWLVKPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVLAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(29301..29753)
                     /locus_tag="RH033_001504"
     CDS             complement(29301..29753)
                     /locus_tag="RH033_001504"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV2990712.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFLRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFITRIGHYIDMLFVEPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGTWFTNFYMRYKPQH"
     gene            complement(29750..30805)
                     /gene="dinB"
                     /locus_tag="RH033_001505"
     CDS             complement(29750..30805)
                     /gene="dinB"
                     /locus_tag="RH033_001505"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDV2990713.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(30876..31661)
                     /gene="lafU"
                     /locus_tag="RH033_001506"
     CDS             complement(30876..31661)
                     /gene="lafU"
                     /locus_tag="RH033_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HDV2990714.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAKIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRAR"
     gene            31633..33345
                     /locus_tag="RH033_001507"
                     /pseudo
     CDS             31633..33345
                     /locus_tag="RH033_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(33620..34369)
                     /locus_tag="RH033_001508"
     CDS             complement(33620..34369)
                     /locus_tag="RH033_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDV2990715.1"
                     /translation="MSSFLHGRFLHPGVFSLCVLLPLLASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAKSRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LVENNHFLSSDHLHNITEVAIHRLEQQLGKPYIWGGTRPDKGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            34681..35421
                     /locus_tag="RH033_001509"
     CDS             34681..35421
                     /locus_tag="RH033_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HDV2990716.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEEK"
     gene            complement(35392..36159)
                     /locus_tag="RH033_001510"
     CDS             complement(35392..36159)
                     /locus_tag="RH033_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HDV2990717.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFFLGERVV"
     gene            complement(36364..36942)
                     /gene="lpcA"
                     /locus_tag="RH033_001511"
     CDS             complement(36364..36942)
                     /gene="lpcA"
                     /locus_tag="RH033_001511"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HDV2990718.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            37182..39626
                     /gene="fadE"
                     /locus_tag="RH033_001512"
     CDS             37182..39626
                     /gene="fadE"
                     /locus_tag="RH033_001512"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HDV2990719.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAVLGVAGLWS
                     AWVLVPLAIILIPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTTEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHSFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKNHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDQLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMVVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTLSKHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(39669..40115)
                     /gene="ivy"
                     /locus_tag="RH033_001513"
     CDS             complement(39669..40115)
                     /gene="ivy"
                     /locus_tag="RH033_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HDV2990720.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            40296..41066
                     /gene="yafV"
                     /locus_tag="RH033_001514"
     CDS             40296..41066
                     /gene="yafV"
                     /locus_tag="RH033_001514"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HDV2990721.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQYNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQTYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMAVLREYREKFPAWRDADEFRLL"
     gene            complement(41118..41312)
                     /locus_tag="RH033_001515"
                     /pseudo
     CDS             complement(41118..41312)
                     /locus_tag="RH033_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(41338..41610)
                     /locus_tag="RH033_001516"
                     /pseudo
     CDS             complement(41338..41610)
                     /locus_tag="RH033_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
     gene            41916..42763
                     /locus_tag="RH033_001517"
                     /pseudo
     CDS             41916..42763
                     /locus_tag="RH033_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950586.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT        10807 a        10999 c        10440 g        10517 t
ORIGIN      
        1 ggcgtggata ccggttccgg tgagggtgac ggttcggtac tgtccgggac acactccagc
       61 caggtttccc ggctcgccca ctccggtgca tccggccagc ggatcttttc gtatccatgc
      121 agaatgacca gagcctcggc atacaccatc aggtcaaaag cttcgttggc accgcgaccc
      181 ggcttactcc atttcccgtc actgctccgc tcttcatacg tcagttcgtc gtaaaaccag
      241 ctccccagcc agtcagggaa atgcacatag ccgggacctg gcgagttacg ccataacgcg
      301 ttattcaccc ggtctttcag ggcatccgtc tgaagaagcc agagcggcac atcacctgcg
      361 gcctgcgccc gtcggcccgt tcgtccggtg ttatcaggga atgtacggct gatcagtttt
      421 gcgcgccgga tgctgtcgcc cttaaacagg taaatacgtt taccaaggcc atcacgacgg
      481 caacgacgcc agaatttata ggcattatca gtgaccccgt cttcaccgcc ggagtccacc
      541 gccattgcca taagtcgcat ttgttgagaa ggatcggagg ccagcggcca gcttttatga
      601 aaaacatctg tcagcaggac atcccagtct tccggatagc tggccggatc aattcgctgg
      661 ctctccccgt cgcagtcact gcgcaatgac tgcgtgatgt tgtaacgatc aataatccag
      721 cgttcgccac gactgccata gcctgttacc tgaaccacaa aacggcgatg acgtcccgcc
      781 tgcacatcca ctgtcgccac aaggaaatta acgccatccg gcacactgcg ggaaggaact
      841 ggctctgccc gctgctcaag cagttcactt tttcgttgct ccatgttggc acgaggaaga
      901 taaggcaatc cccagtcggt gttgataacc gccctgagtg tttcttcgct tcctgtcgct
      961 tcatactcct gttctgcagt cagtaatttg taaaccagtt gcgcccaggt ctgatacgca
     1021 gcagctggcc cttccatcca gaaactggcg atacgggagc ggcgcggttc accggaaacg
     1081 ttgccgttac gatcaatgac ctgaccttca cgcaaccaga ctcctgcact attgagctca
     1141 cgctttttct ccgcagtgat aatgccgctg cagtgcgggc aaagtagata cgccgcctca
     1201 ctggctttaa agggatccgg ttcattacgg tagccggtca tggcatccat ggctggctga
     1261 aaatattcac cgcagtgcgg acatggccag taccagcggc ggcggtcacc acgattgtaa
     1321 agggaaagaa taccagtcgt cggtggcgct tcatgaggcg acttacgtcg ccatttgctg
     1381 tcgcagatgt cacgtcccgg cgagctctcc accagagtca tcccggcgga cataaatgtg
     1441 gtggtacgtt ttgaggccag ggagaaacca tcaccctcgc tgtcgatatt ctccggaaaa
     1501 cggtcgtaat cggttaaggc gacaaaccgg taatccgacg acgacataat gttgaccgag
     1561 ggccaaccaa ttttaaggaa cgagccatcc ctgaacgtct tatcatgaac attattgtcg
     1621 ttacgacgtg gactcattct tttctttacc gccgcactgc ttctgaacgt tctgtcgagg
     1681 cgctttttag aatgctcgcg ggccttatct tcggtcatct gcacaacgag catgtccgaa
     1741 ggatcgcaaa cgatggtata gacaatccat ccatcgatca gaccaatggt cttccctgtt
     1801 cgcgcaggac caacaaaaat caccgcatcg tattcacgcg atgccaggca gttcatgggc
     1861 tcaatgatgt agggtgtcag ttcaggatcc catggcaccg agttaccagc ccccttggga
     1921 acacgcatga attttttaac agcctccgaa atcggcatgc gacgtggtgg ggaaaatcct
     1981 gccgatatgt cccttcccaa atttcgggct gatgaaaaac ccattattcc tcctagagac
     2041 tctctccttc ctcatcagga attaattcag cagcacaagc ctcgtaggat ttttcctgaa
     2101 gagtgtatcg caggtcatca atggcctgct gtacaacgcc gacggcctga ggagtcaggg
     2161 cgcaatcgcg ttcaagaaca tccggaattg tctccagaac ctggacgaca gcctttctca
     2221 tggacgaata gacgatgact acttcatcaa cggggatgag ttttcgctgc tccttttcca
     2281 gcttgatcct ttcattttca gactggtacc aatcctttct ctctttcggc tccatacggg
     2341 atggatcatg aacagagtct gctgcctcat gcttcacact aaacagggca ggcccgacat
     2401 gctgcagggc gtaaacgggg ttccccctga cagtcgctgc cacaggagtg ttggccgcga
     2461 ggagccgttt ttttactgtg tcccggtgaa gcccaaaggc ctcggcgatt ttaaaaacac
     2521 tccagtaata agcatcaccg atcccgctca catttgacat aagcaactcc atctggcagg
     2581 tgaaaatcag gtttatttat atatttcaat taattgcaaa ctggtctaat gacagggaga
     2641 ataaaatatt gtacaggtga aaagagaaat aacttttaat tatcaataaa ttacctaaca
     2701 tgctgccgcc gccatggaaa tgcaaaaact agcctttttc cgcgacgctc ccgccccgtg
     2761 agagctcagg tcatcagaag gacccacatt aaaggcagaa tattcactct gatattacgc
     2821 attgttttgc ttttgtagat tcgcaattta cggtaaaaat acttttgcta tttcttcgta
     2881 ccataaatgc acaggagtat cttcatgtcc ataaaggtat tcagaagtga ccttcagact
     2941 gagagtgaat cagactatcg taattggctt ggcgataatc cagacggata tgttgtcaat
     3001 gcattgaagc gaaccagagg taaaggtaat aaatctgacg aacgctttac cagaatccat
     3061 agagcatctt gcaaaacgat taatcccttg aaacgcaaga tcgataaaac aggattcaca
     3121 actggagatt atcaaaaaat atgctcaacc aacttcctca atgttgaaaa tgaagcaaag
     3181 aaaatgacag gtctgacaag catcatcaaa tgtcgatgca tctaaagcgt catgtaatta
     3241 acatgttcac aacactctcc gggtgaccgc taatgctgtt gtcagccaaa aactctacgt
     3301 ttcgattatg ccaattttgc accgcccgat gccgataaat gcgaaaatcc ggcgactatg
     3361 tagcgtaatc aaatcaacga gataccactt tcatgaaata attatcatct gtcagttcaa
     3421 tatcaatgtt gagagagaat aaatgagagg aatgccaaga ggaagtcgag gacagagggc
     3481 tgtccaatca actgcaagaa gagcaagacg atcaaatgcc agccattctg accaattagg
     3541 atgcctggca ctaatcatcg ttggcctgat atttatctat ttcatggcta attagcaaag
     3601 attgctcatc ttacacactg cgtgcgaatg tattgctgca agcctcggat cattttatca
     3661 ctggtttcaa tccgctttct aagggtgaaa taatcccgtt gagcggtgtc agtaagtctg
     3721 gggctggtgc catcatccac gccggaggcg gcggtgattt tatgcatgtc tggacagaca
     3781 gctttgacgc gcagccactt acgcccagca gaaacatcag cacgaagact ttcgatagtc
     3841 gcgttagcat cagcaagctc ctttgtgtat ctggcgtcga gttctgctac atcacgttga
     3901 cgcttctgca tatcagcgat agtggatgcg gctttgtcgc gctgctcttt gtaggcgatg
     3961 gcgttatcac ggtaatgatt aacagcccat aacaggcaga cgatgatgca gataatcaga
     4021 gcggagataa tcgcggttac tctgctcatt gctgccccca caaacagact tcacgctcaa
     4081 tctcacgacg agtcatcagg cctttccatt gcttaccgcc agcgtatatc cagcgacgta
     4141 gctggtcaca tgcgcctttg atatcgccct ggtttatttt gcgaagaagc gtcgatgttc
     4201 tgaaattgcc agcacccacg ttgtagacga acgagtaaag agcgccgcgc gttgtttccg
     4261 gtatatcgac tttgatgtac gggttaattt gtctggcaac cgtggcaagg tctttattca
     4321 ggagggcttt gcattctgct tcggtatacg ttttaccgag catgatgtct tttccggtgt
     4381 gtccgtgaca tacagtccat acgccaacga tatctttgta tggtatgtag ctgacacctt
     4441 ccaggccatc gtcaccactt gggccagtga ttaacactga tgctatagca attgctccgc
     4501 caccaatagc agcagcaact gcttttcgta atgatggagg cattattcac ctctcgcagc
     4561 cttgcgctta tcttctttaa tcttgaaata aaggtttgtc aggtacgtca gcaggccaaa
     4621 taccagacta cccagcacac ctattgccgc ccactgtgag ggcgtgactt tatcgagcaa
     4681 ctgtaaaaac cagtacccgg cactacctgc tgaggtgcca taggcgacac ccgttgttaa
     4741 cttatccatg gatttcataa ccccacctcg cagatgcggg tgctgtgtaa tggaaataaa
     4801 aaggccacct gacgtggcca ccagattatt tccccaccag ctcgtttatc tctttcactg
     4861 tctggttaaa ccgctctgac tcaagctcaa cacctaaggc ccgacgcccc agcgccattg
     4921 ctgcttttat tgtggaaccg gatcccataa aaaaatcagc aaccagatca ccaggtcgac
     4981 tactggcatt gattatttgc ctgagcatat ccgccggttt ctcacacgga tgtttccccg
     5041 ggtagaactg aacgggttta tgcatccaga catcggtata aggcacggag actgatacgg
     5101 agaaatagcg ccggagagat ttaaactcat ccagcaattc agaatatttg cgattcagtg
     5161 aatcataaga tgccaccagc tggtggtgtg gttgttccag ttgttgttcc tgaaacttct
     5221 ctgccgctat acgggaaaac agtgcctgta acttccgata gtcagcctca ttcggcaact
     5281 gccactgact ggcaccaaac cagtgggaaa ccatattttt cttacctgtg gcttcggcaa
     5341 tttgttttgc cgttataccc agttcggcac gagcatccct gaaatacgat atcagcggtg
     5401 ccattatgtg ctgtttgagt tccctttctt ttgctgcata gccgtcactt ttgccgcgat
     5461 atggcccctg gtaatgttca gcaaacagaa cgcgctctgt ggcaggaaaa tatgcgcgca
     5521 gactttcttt attacaccca ttccaacgtc cggacggctt cgcccagatg atatggttaa
     5581 gcacgttgaa acgttcacgc atcatgatct caatatcaga tgccaggcga tgcccacaga
     5641 acaggtaaag gcttccggca ggttttaaca cccgccagaa ctgggccaga cagtggtcca
     5701 gccacttaag gtaatcttcg tcccctttcc actgattgtc ccagccgttg ggtttcacct
     5761 tgaagtacgg cggatcggta acaatcaggt caatggaatc atcaggcagg gactgaataa
     5821 aatgcaggca atcagcgttg attaaatcaa cactgtttat ttttacagta tttttcatgg
     5881 atcagtaagc gtaactctgg taggctcact ctgcttttgc gctaaagcag tgggccgtgg
     5941 ttcgcttgtg accagtaagc atgagcgaat ggctggcagg tgctaccaac acccaccagc
     6001 cgcccatttt cacagcagga aaccgccatt actggcagcg tctgaattta ttcccgtacc
     6061 cgccgttatc cttcgccaga cccgccagaa ctaactgagt cagtattaac tggcaccggg
     6121 cttcgcttac tccggtagtt ctcgtcatca tgcgtggcgt tacccacttg tcagcaggta
     6181 agaaatgaag gactgctgcg gcggtttctg tcatatcttg ctgttttagc atgtcttttt
     6241 cccttctggt taacatgaca taccaataac tcttgtctaa aaagccagca agataaaaag
     6301 tcagtattca cgaccaccag cgtgtttact gtactgcacc aggtatacag gtacaaaaaa
     6361 accgctcagc ggcgggttta agttgtgtgg cgaagtaacc actcttaaca gcatatttga
     6421 ttttttacga ttgtaaacgg ttgattattc atctccaata aaaataattg tgtgggtatg
     6481 cccttaacaa tggataagaa acatgaataa aatgactgta ttattactta gcgcaactat
     6541 catttcaggt tgtacttctt ccgtaccatt gataaagaaa actcaatcag gaaaacctga
     6601 ggggttttat caaaatacga caaaagataa agtcaaagat gcccttgtga attactgcaa
     6661 tagtagaggg ttgataattt acaacgcgga taacagcagt gttatatgtg gtaaagaact
     6721 ggaaggcggg tctgctgttt ttggacaaat gttaatcggc aatgcctatt caacaacccc
     6781 ggtatcaaaa gtcaaattta ctatcgctca agttaataac gatacaaaag tgtgggccga
     6841 tatgtggatg gaaactcaaa tggcaatggg gcaagtacaa caaatggcta taacagacaa
     6901 cgcaagcaaa aacactatcc aacaacgtct tgatgaatta aaaccttaag taaattaatt
     6961 aaataaaatg gggagaataa atcgactccc cacacattaa actgattcaa ttaccccctc
     7021 aataagaggt cttctaaaga tccatctcta gctcaatttc taacatcatt aacatgccat
     7081 caactacacc ttcagccttt tgcaataaac gcccaaccca gcaatcagaa cgcccatgtt
     7141 tacgggcaag cgccataaac gtcataccac ctacataata atccactaat aaatcgtgca
     7201 aatcgctgtt gttctttttc aggcgagcca tgcatccaca aatgatcatc gcgtcatcgt
     7261 cacaacattg cgggcgggat tttacttttg aagggattag tcctttaaat cctgcagcaa
     7321 tagacgacca agtgacatcc tcgtgattat ttgccaccca tgccccccaa cgttcaagaa
     7381 ccatttgaat atcacgcatc aactttctcc acaaaatcag gccagtacgc caattgccag
     7441 cgcacgatcg ataaaacgaa atatcagctc cagctgggag ccatacttct cttcaaatgc
     7501 cacggtatcc gcatgcagct cgtcgtgatg ctttctgcac aaaggcaaca caaaaaggtc
     7561 atgcgctttt gttcccattc caccctgacc gtgacctatc aggtggtggg gatcatcagc
     7621 gggctttcca caacatgcac acggctgtgt cttaacccag cgcgtgtact tttcattaac
     7681 ccagcggcga tgttttgggc gtaacataaa agactccggc gactccggat ccactttcag
     7741 cgccagcacc tttttcgcct tatcctggat gatgctggtg gcaggaaccg aaggcacaag
     7801 gtcactttcc cgggtaacag acggcacaac aggcttcggt aatctcagtg ccttacgggc
     7861 tgcactttcc ggtaaggcat ccgccagatc attacgaatc agccaccagc acagttccgg
     7921 cattgtcaca acgtgactgt catcaaaacc gagatcccga cgcacaacag acaacaccca
     7981 gcgggcacag ttatccgttg ccattgattc cagccgttcc gtgaactgat cgcgcagctg
     8041 gttatcgcag tgccagcaca gacggattgc acccggagcg tgtcgcattg tggtcatgtt
     8101 ctcgctgtgc cagtcggaat gaggccactg gcagcctttt tcacgaagta accagctttc
     8161 aagacattcc acgccaccag cacgacggat cactgcctca ttgcggaaca cggcccgaac
     8221 ggcaggatca tccgccagcg gttgtgatgc cgccggaacg gcaccactgg cgaaagatga
     8281 ataacgctcc ggctcaggct ccagcaggac acgcccctgc ataaacaggg gcatcagctc
     8341 tgaacctggc ctgaacaata cgatccccat acgcggggca atttcagggg tcagtagtgc
     8401 tctcacggtc acctcaatga acggtatcga gcagctttaa cagctcaggg aatcgggatt
     8461 cgaagaaatg cggctgcgtc tcgcgcggat ttgcgggact ggtgatgttc ttgccgaaca
     8521 tgcaaccttt cgctgtcagc gaccagaatt ttttgatgtt gttaatcgcg gtacggctgt
     8581 atcgttcgcg ctgctcgacg atccccagtt tcaccatctg gtgatatgcc tgattagccg
     8641 tcaggcgtat accatactgt ttcagcagtg cactcagtga cagtgtcggg cgacttgagc
     8701 catcgtgtgc atcagcagga gcatcaatgg catagcgcgg tgccagattc ggtaagccaa
     8761 cagcctcctg gagtttctga caggcaccaa gcactgaaga gttagacagg tttaactccc
     8821 ggcgcataaa gtccagcagg atcacgccag cctgcatctt gtcagcagcc tgtccggata
     8881 atttttccgg tgcgctggtt accatatcga aagtacggat caccttcaga tggaatgacg
     8941 ggctgatcca cattgcatag gcatacacca gttccttgca gacatacgtt ccccgttcat
     9001 ttcccccatg aatcacactc accgggtcaa cacccaaatt ctgggtgttg gtcaattcat
     9061 gaacaagctc aacagtttgt tggctggaaa gaaactttcc cggctccttg gttctggcat
     9121 ttgcaccaga tgctactgct gcgcgatgca gatcgttcag gctgtaacgc ccataagcat
     9181 cacgacgaac ttcaatacca tcaatgacca tcagattatt catacttcgt ttctcctctt
     9241 aatcaggcgg ctgcacccgc cgttttctcg tacttactga tagtgatctc gaccttccct
     9301 tccgggataa ccggtcccca ctccaccagc attcttttca cctgactgtc gtcttcccac
     9361 acacccgcgt gggtcagggc gtcaaacagc gccttgttat agttgtccag atcgcggatc
     9421 cggttatccg gaggaaacaa cacgatctcc actgaagcag gtgccgacgt tggtttcggc
     9481 agacgacgta actgctcaac tattgctgcg cacgccgcgc tctggaattt tcgccccgcc
     9541 gcgcttatca ggctcttacc agcaaacgcc cctttgttgg ggtgtcgcca gtacgtgttc
     9601 acgctgggcg gaaaaggcag gatcagcttc atactttcag gtccctctca tgtaaccagt
     9661 gggttgcacg cagccttgcg ttttcctcac cggcaagcag tgcgcggata atcccgaccg
     9721 cctcgctgtc gtcgtccttc accgcggtat gaagcgttat cccccgggcc acgccacgct
     9781 ttatcgtgat gacgcctttt ttctccagtg cgcgaagatg ctccaccgct gcattcaccg
     9841 aacggtatcc cagcatggtt gccacctcct gattggttgg cgggaagcca cgttctttct
     9901 gataagaaat cagcatatcc agcacctgct gctggcattg agttaacgtc gtcatgccgc
     9961 catctccctg accagttttt ctgcctgctg gcgaacctgc gccagaaacg cctcaccaca
    10021 tgcctcaagt tcatcgcgcc cgatgtagct gattgccggt cccttccagg tcttgtcgaa
    10081 aacagcaata gcaccagcga agaaagcgcc tgtcggcacc tgcttctcgt ccttcgggat
    10141 aaaccaggca ggcagttcaa aaccaatacg cccgcgaata aaagcaatat gatctgcatc
    10201 ttccggccac cacacttcgc tggtggcagc tttgatcagg aaaacatagc gtccgccctt
    10261 atcacgcatg gcactagcat gcttcatgat gtaacgcatg ccggtgatgt attgcccttc
    10321 atgctgactg gcgcggctgt acgggggatt accaaaggca gcacctttaa gctccgcaag
    10381 acgttcagac cagtcatgcg ccagcgcgtt gtcttccgca gtgtaataag cggcacattt
    10441 ggcgttatca ccatcagtga acagatccag aacaaacggg ccaaacaggg tgttaattcc
    10501 ccagaaaatg ttatccggcg tgcgccactg atcgcccact tccttcagtt catgggctgg
    10561 tttgttccgc agctccacca gcgcctggca atatttatta ctcattaagc ccccacgtaa
    10621 ttccctgaca gataccactc ttcacccgat gcagcgcgct tgctgctttt ccgtaagcac
    10681 cgctcacgac gcgccagaaa attgtttcgt tctggctggg agtggctttc acggaatgcc
    10741 gccatccaca ccgttgcagc acgacggtat aagcccctgg actccagttc ttccgcctgg
    10801 cgggtcaggc acaaaatcac ccggggatcg ttagtgccga catagaaatt gcgcacaggt
    10861 ctggtttcac gaactggttg tggttccggc tcctgcgctc tctcagtcag gcgtgggaaa
    10921 tgtctgcgtg tatctccttc acaacggtga gccacacgcc cactctgacg taacttgctt
    10981 gctgactgca gtacgcgctg ccgtgagtaa cctgcaaaag catccgcaat gtctccggaa
    11041 gtacagcccg gatgggcttc aatgaatttc tgaacgtcat tcaaaagact catgatcacc
    11101 ccctgaatcc tgccgggatc tggctgtagt ccacgttgtc gtaactggct ttgaagtacg
    11161 ggtcttcgcg tttttcggtg tacgtgctga cggacggcga taagcgcagg gaaagctcat
    11221 cccatttttc ccgcaacttc gacgggctga gcacgttacg gcaccagaac ggatcgcggc
    11281 tgacgcggct gtacatctcg cagatttgtt tgtgagtacg cccatcctgt acacacatca
    11341 ggcgaatttc gtttgcccag gctgtccagt tcggttcttt gggacgaacc acctcgccgt
    11401 cacattcggc agcctgctcg tacagggcga tgattttttt ccagagccac tgtgcgcagg
    11461 tcaaatcatc ctgcgtcccc cactggcgct ttttagggct gaatacaacc gcatcaggat
    11521 ggcgagttaa aaaatcctgt tcagccgtct gcgtgtccgg ttgcgaagcg tccggacgag
    11581 aaggtttttt atctgacgga tcatgttttg attttactga cggatccccg ccagattctg
    11641 acgggtgaaa acccgctttt ttgccagatt tcgacgcatc aaattttgac gggtcagatt
    11701 ttgatgcgtc agattttgat gcgtcagatt ttgacgggtc agaatctgac agttgagaaa
    11761 atgccgctgc ctgaagcttc gcaacgttaa gctgataaac attcgacgca ttgcggttac
    11821 cctggcgacg cgccttacgc gttaaccagc cttctgcttc cagccgtgcg atagccgttc
    11881 tgacggtact catccccgct ccaatctgac gggcaatggt ttcaattgat ggccagcaca
    11941 caccttcgtc attactgaaa tcagccaggc gggccataat tgccacgctg gataacttca
    12001 tgcctgatgc agcgcaacca tcccatacat agccggttaa tttagtgctc atgaccgacc
    12061 tctatttccc tgaatttacg acgaaactgt tcgagcggac tgaagcattc atgctcataa
    12121 ccttcgcgga ggtagataac ccgttgtgtt tccggctccc aacgaatgac tctgacgggc
    12181 actccgtagt gatctttgaa ccagcggtta acttgtcgca aaggactgtc tccttctgcc
    12241 ggttgaaatc acccacagcc cactctgcaa agctgtgggt tacaatttcc ctgtcacctg
    12301 gtacattcac tgcatagcaa tattccacct tcgcttttcc acccggtaca ggaagcgcaa
    12361 tcagttgcga gcgacggtag tgtgttgtta aactgttcat gcgttagttt ctccacaacc
    12421 agaagcaatc gacgccacga cgcccggagc tgcacactcg cgggcgtcat tactttctga
    12481 aatgcaaaaa attttgtaga caagtgctgc atgctcctgc agcttcgaaa ttgagagata
    12541 cagctcgtcg ttaattgctg tcttctcatg cggttccact acaccgtctt cgattgctga
    12601 acgaatctgt tttgaataac tgccgatctg ttcaatgact tccagcagac gctggttaat
    12661 atcggcgttg tccacatcct cgacgtcagg aagagacaca aagacgccat ttgcagactg
    12721 cgccacagca tcagcaatga agtgagtgcc accagcacgc tgtaaaacca ttgcccatcc
    12781 cagcgggaaa atctgatcgc catctgcacg aaggcggttg aataaagcgt tttctgttac
    12841 atcgagccag tcagccgctt cagcgtaacc acccggcaac gccgcgatag tttttctgac
    12901 agctttcacg taccactcag gctgtttttc tattttccag tgatgcttac ccacgattag
    12961 cctcatcgtt ctgtggttaa aaattgaaag tgttctgcta atctttcgga tagatatccg
    13021 gtcttaagtc agatttcgta attgcacctg acgtgcattg ctcaagtttt ttagccagca
    13081 caaaactggc ttttttatag ccattgaaaa ccagccgtaa gtagccaggt gttgagccaa
    13141 cttttccggc caactcgccc tgctgttctt tggttaaaga gtcccaatac gctttcatac
    13201 aatatgtacc tccggtgtac atattacatg attgaaatga accttcaaga tacttgtacc
    13261 ttaacggtac aagggtttta atttcgttat gaaaacaatc catgacatcc ggcggtctaa
    13321 cgccagaaaa ctgagagatg gtgttggcgg gaattcttcc tttgccacta tgattgatcg
    13381 cgagccaacc cagaccagca ggtttatggg agatggtgct actaaaaata tcggtgacag
    13441 catggcacga cacatcgaaa aatgtttcga cctgcctgtc ggatggctcg atcaagaaca
    13501 ccagacaacg aacatcacaa aaaaacctga tgtttcaatc actaataaac aaatcacatt
    13561 agtccctgtc atatcatggg tacaggccgg agcatggaaa gaagttggat attctgaggt
    13621 tgatttgagc acagcagaaa cgtatccctg ccctgtaccc tgtggggaaa tgacttatat
    13681 cttgcgggtg ataggtgatt caatgattga tgagtaccgc ccgggagaca tgatttttgt
    13741 cgatcctgaa gtacctgcct gccacggtga cgacgttatt gcattgatgc acgatacagg
    13801 tgaaaccacc ttcaaaaggt tgatagaaga tgggacacag cgttatctca aagcgttaaa
    13861 cccaaactgg cctgagcctt acattaagat caacggtaat tgctctataa ttggtacagt
    13921 gattttctca ggaaaaccaa gaagatacaa aatcaaagcc taatcaatgt ttatgaacct
    13981 gcttcggcag gtttttttat acttgacaat gtacctttga gatacataat gtacccaagc
    14041 gaaacaacga acaggcagga cgcccacgaa gtagccgcct ggggcatatg aagtccagga
    14101 tgattcatta gcaacaaaaa agcgccctat aggacgcttc gctctttaac aatctggaga
    14161 tccccaacac ttaacgggat tttggcttcg gttctggcgg tgaaggacga ggttttaagg
    14221 gtatatgatc ctctttccat gtatatataa atgcattcat ttggtcatct caatatagaa
    14281 tccattatta attcatactc ataaagcctg tgtgactttg gtgatggtgg tctgggcttt
    14341 ggaggtacat ggttagggat tatgggaatt ctatttgtca ttattacacc tttttgaccg
    14401 aggcaaatct ccagcaaacc atgcgataac gctatgcatg aatgatattt ctgactcatc
    14461 aacacgtccc agcgcaatac aggtccgttt gaacgcagca tcttgtaaag atctccatgg
    14521 attactatca gcgtcttgaa cctttaaaaa ccgagtgcgt agttcgtcat cagaaaggga
    14581 ggaaaactca acaatcaacc ttttatactg tcgcatctgt tcctttgata agccagcttc
    14641 ctgcccaaat tggtatacca attgaagcac cgacaaaata gctacagatg cgccaaaaat
    14701 aaacatattg ctatatggcg caaaaacaga aaagcccaag acaatgagaa caagagttat
    14761 gctcttgtct atccgattta aaagggtgta attcattttc tcaagaaaga aagaataatg
    14821 aatatcgaat gctagatcgt ctcgagtcat ctctattccc ttacatttca ggtttcggct
    14881 caggggtcgg tgctggtctc tgatttaccg gaacatgaaa tttttcattg ctatctacgt
    14941 acatatttcc ttcctacctg ttgttgggat atccagatta taccgaatcc ttgttgttgg
    15001 ggaataacca ggtccacctc gcctgatgtg gctaaaagca ggcacataac agctaagtat
    15061 tttcaaccag agagaatcct tagcgttgtg gtgaatgcgg ctcagcgcac gcgggttaag
    15121 gttgaggctg acagtcgacc ttctgtggat acccacccgt ctggtgtaca accttcgcca
    15181 ggcaccggga ggcacccggc accacaactt tatgctgtgt gtagtcttgg cggtaccagc
    15241 ttgtaccctt gcttccggct ggtaccgtcc tttttacaaa acagagaaga gcatcaccgg
    15301 acgacgggct cataacccaa tccatccggg cggcagtcac cgcaggtgtt cttctctgtt
    15361 ttgtggagaa actaaccgac cttgcagggt cgatatgatg aggagcagca aaatggctag
    15421 cgaacgcagt actgatgtgc aggcatttat cggggagctg gacggcggcg tatttgaaac
    15481 caaaatcggc gcagttctca gtgaagtcgc ttccggtgtg atgaacacga aaaccaaagg
    15541 taaggtctcg ctcaacctgg aaatcgaacc gtttgatgag aaccgagtga aaatcaaaca
    15601 caaactctca tatgttcgcc cgactaaccg cgggaaaatt tccgaagaag acaccaccga
    15661 aacgccgatg tatgtcaatc gcggtggtcg cctgactatt ctgcaggaag accagggaca
    15721 gttactgact cttgccggtg aacctgacgg aaaactccgc gcagcaggtc gttaatatcg
    15781 tttttaatta actgattatt tatctcatca ctgaatatct ttatatagtg aggacttatt
    15841 atgtctcaga acttagacgc aaccgcaatt aatcaaatcc atgcccttat ttctgctcag
    15901 ggtgttaatg aaattatcag taagattggt gccgatgctg tggcattgcc tgagaatttc
    15961 cgcattcatg atctggaaaa atttaattta aatcgcttcc gtttccgtgg tgcgctttcc
    16021 actgccagca tcgatgactt tacccgttat tctaaagatc ttgcagatga aggcacccgc
    16081 tgctttatcg atgctgataa tatgcgtgcc gtcagtgtgc ttaacctggg tactattgat
    16141 gaaccaggtc acgcagataa caccgccaca ctcaaactga aaaagacagc accgttctct
    16201 gctctgttgt ctgttaacgg cgagcgtaac tcccagaagt cactagcaga atggattgaa
    16261 gactgggccg actatcttgt gggctttgat gctaatggtg acgctattca ggcaacaaaa
    16321 gcggctgcgg ctgtccgtaa aatcacgatt gaagcaaacc agaccgctga ttttgaagat
    16381 aatgacttca acggcaaacg ctccctgatg gagtctgtcg aagcgaagac caaagacatt
    16441 atgccagtgg catttgaatt taaatgcgtt ccgtttgaag gtctgaaaga acgtccgttt
    16501 aaattacgcc tcagtattat cactggcgat cgtcctgtac tggttctgcg cattattcag
    16561 ctggaggcgg tgcaggaaga aatggctaac gaatttcgtg atctgcttgt tgagaaattc
    16621 aaggacagca aagtagaaac ctttattggt actttcaccg cctgatttca ttactgcaaa
    16681 tgcccctgcg ggggcattta tggaaacgta atttactcaa taatcgccgg atggtgaggg
    16741 attcttttta ccagaattca gcgcggtgca gcgcatatac gtggagaaca aaatgtcatt
    16801 tattaaaact ttttccggga agcattttta ttatgacaag ataaataaag acgacatcga
    16861 tattaacgat atcgcggttt ccctttcaaa tatctgtcgc tttgccggtc atctttcgca
    16921 cttctacagc gtcgcccaac atgcggttct ttgcagccag ctggtgccgc aggaatttgc
    16981 ttttgaagcg ttaatgcatg atgcaacaga agcgtattgc caggacattc ccgcaccact
    17041 gaaacgcctt cttcctgact ataaacaaat ggaagaaaaa atagacgccg taatccgtga
    17101 gaaatacggg ttacccccag ttatgagtac gcccgtgaaa tatgccgatc tcatcatgct
    17161 ggcaaccgaa cgccgcgatc tcgggcttga tgatggctct ttctggcctg tactggaagg
    17221 tatcccggca acagagatgt tcaacgtgat tccactggca ccgggccatg cctacgggat
    17281 gtttatggaa cgctttaacg agttatcgga gttacgcaaa tgcgcatgaa tgttttcgaa
    17341 atggaagggt ttcttcgtgg gagatgtgta ccgcgagatc tgaaagtaaa tgaaacagat
    17401 gctgaatacc tagtgcgtaa attcgatgcg cttgaagcta aatgtgcagc acaggaaaac
    17461 aaagtaatac cagtgtcaac tgaactgcca ccagcaaatg aaagtgtttt gttattcgat
    17521 gctaacggag aaggctggct aattggctgg cgttctctct ggtacacctg gggacaaaaa
    17581 gaaaccggag aatggcagtg gacatttcag gtcggggacc ttgaaaacgt caatatcact
    17641 cactgggcag taatgccaaa agcaccggag gctggagcat aatgaccact tttaccgaca
    17701 aagaactgat taaagaaatt aaagagcgta tcagcagcct tgacgtgcga gacgatattg
    17761 agcgccgtgc ttatgaaatc gcactcctat ctctggaagt agaaccagat gaacgcgaag
    17821 cttatgaatt attcatggaa aagcgtttcg gtgacttagt agatcgtcgg agagcaaaaa
    17881 acggcgataa cgaatacatg gcatgggata tgactctcgg ttggatcgtc tggcagcaac
    17941 gagctggtat ccatttttca acaatgtcac agcaagaggt gaaataatgg agccatacag
    18001 cctcacactc gatgaggcct gtcattttct caagatatcc agaccgactg ccattaactg
    18061 gatacgcaca gggcgtcttc aggcaacacg caaagatccc actaagaata aatctcctta
    18121 cctcacaaca cgacaagcct gcattgcggc tcttcagtct ccgctgcata ctgtccaggt
    18181 gagcgcgggt gatggcataa cagaggaaag aaaatgtcac tcttccgcag aggtgaaata
    18241 tggtacgcca gtttcacatt gccgaacggt aaaagattta aacagtctct tggaacaaag
    18301 gacaaaaggc aggcgacaga actccatgac aagctaaagg ctgaagcatg gcgggtcagc
    18361 aaacttggtg aaatacctga tataacgttc gaggaagcgt gtgtcaggtg gcttgaagag
    18421 aaagcacata aaaaatcact ggacgatgac aaaagccgga tcggattctg gcttcaacat
    18481 ttcgcaggaa tgcaactaag agacattact gaatcaaaaa tttattcagc aatgcagaaa
    18541 atgacgaacc ggcgtcatga ggaaaactgg agactcaggg cagaagcatg cagaaaaaaa
    18601 gggaaacctg ttccagaata cacgccaaaa ccagcgtccg ttgcaacgaa ggctacgcat
    18661 ctttcattta taaaggccct actaagagcc gcagagcgtg aatggaaaat gctggataag
    18721 gcaccaatta ttaaagtgcc tcaaccaaag aataaacgga tccgctggct ggagccccat
    18781 gaagcacaaa ggctgattga tgaatgtccg gagccattaa agtctgttgt tgaatttgca
    18841 ctggcaacag gcttaagacg ctcgaacatc atcaaccttg aatggcaaca aatagacatg
    18901 cagcgccggg tggcatggat aaacccggaa gagagtaaat caaaccgcgc aattggcgtt
    18961 gcgctgaatg atactgcatg tcgcgtattg aaaaaacaaa tcgggaatca tcaccgttgg
    19021 gtatttgtgt acaaggaaag ctgtaccaaa ccagacggaa cgaaagcgcc aacggtcagg
    19081 aagatgcggt atgacgcaaa cacagcttgg aaagcggcgc tgagacgggc tggtattgat
    19141 gatttcagat ttcacgactt gagacacacc tgggcaagct ggctggttca agccggagtc
    19201 ccgttgtcag tgttacagga aatgggaggc tgggagtcta tcgaaatggt tcgtcgatat
    19261 gctcaccttg cacctaatca ccttaccgaa cacgcacggc aaatagactc gatcctgaac
    19321 ccatcggtcc caaatttgtc ccagtcaaaa aataaggaag gtactaatga tgtgtaactt
    19381 attgatttaa atggtgccga taataggagt cgaacctacg accttcgcat tacgaatgcg
    19441 ctgctctacc aactgagcta tatcggccct gaaaggacat gttcacgaac gtgaatcacg
    19501 gtggacaagg ttaaaactaa ccgggcgatg cgtcaatggc cttgtgaatc aaatggctac
    19561 ttttgcatca tccggtttta tttacgcacg aatggtgtaa tcaccaatgc cgatccactt
    19621 ataagtggtc agtgcttcca gccccattgg gccgcgcgcg tggagttttt gtgtgcttac
    19681 cgcaacttcc gcccccagtc caaactggcc gccgtcggta aaacgcgtag aggcgttaac
    19741 gtaaacagcg gaggaatcca cttcgttaac aaaacgctgg gcgttgcgca tatcgcgggt
    19801 caggatcgca tcggagtgtt gcgtgccgtg ttcacgaata tgggcgatgg catcgtcaag
    19861 atcgctaacg attttgacgt tcaaatctaa tgacagaaac tcatcgtcat actcttcggc
    19921 tttaaccgcc accaccttcg cggggcctgt ctgcaactgc gccagtgcac ctgcatcagc
    19981 gtgtaacgtc acgccacttt ccgccatttg tttgcttaat gcgagcagga aactatcggc
    20041 gatgttttta ttcaccagca acgtttcaac cgtattacat gtgctcggtc gctgagtttt
    20101 cgcgttgacg atcactttca gggcttcagc gatctctgca ctttcatcaa cgtaaatatg
    20161 gcatacgcct ataccacctg tgatcaccgg gatcgtcgac tgttcgcggc acagtttatg
    20221 caggccagcg ccaccgcgcg ggatcagcat gtcgatgtat ttatccatac gcagcatttc
    20281 actgaccagc gcgcggtcag gattatcaat cgcctgcacg gcacccgccg gtaagctgca
    20341 ggatttcagg gcgtcctgaa tcaccgctac cgttgcagcg ttagtgcgac aggtttcttt
    20401 gccgccgcgc aggatcaccg cgttaccggt tttcaggcac agggaagcaa catcaaccgt
    20461 cacgttcggg cgcgcttcat aaatcacgcc aatcaccccc agcggcacgc gacggcgctc
    20521 cagacgcagg ccgctgtcca gtacgccgcc atcgattacc tgccccaccg gatcggcgag
    20581 attgcacacc tggcgcacat cgtcggcaat gcctttcagc cgtgcgggcg tcagtgccag
    20641 acggtcaagc atcgcttcgc taaggccatt ggcacgcgcg tctgcaacat cctgcgcgtt
    20701 agcgttgagg atactttcgc tttgtgcttc cagttcatcg gcgatttttt ccagcacacg
    20761 atttttttcg cggctggaga gttgcgctaa tttatacgag gcttgcttcg cggcaatgcc
    20821 catttgttcc agcatcggcc agctccttaa cgggtaatca tgtcatcacg gtgaacggca
    20881 accgggccgt attcatatcc cagtattgca tcaatttctt gcgagtggtg tccggcaata
    20941 cggcgtaatg catcgctgtt gtaacgactg acgccgtggg cgatatcgcg accttcgagg
    21001 ttgcaaatac ggatgacttc accacgcgag aaattgccag tcacgctttt aatgcctttc
    21061 ggcaacaggg agctgccgcg ttccagaatg gcggcagttg ccccttcatc taccgtgatt
    21121 tcacccgcag gcggcgcacc gaaaatccag cgtttacggt tttcaagcgg agtcgcctgg
    21181 gcatggaaca gcgtaccgac ggaaatgcct tccatcacat caccaataac gcccggcttg
    21241 ctgcccgcgg caataatggt gtcgataccc gcacggcaag ccacgtcagc ggcctgcaat
    21301 ttggtgctca taccgccagt tccgaggcct gaaacgctat caccggcaat cgcgcgcagt
    21361 gcgtcatcaa tgccgtacac atctttaatc agttctgcct gcggattgct gcgcgggtca
    21421 gcggtataca aacctttttg atcggtcagc agcaacagtt tatcggcacc cgccagaatc
    21481 gccgccagcg cagaaaggtt atcgttatca ccgaccttaa tctctgcagt agcgacagca
    21541 tcgttctcat taattaccgg aacgatattg ttatcgagca acgcacgcag ggtatcgcgg
    21601 gcgttcagga agcgttcacg atcttccata tcagcacggg tcagcaacat ctgcccgaca
    21661 tgaatgccgt aaattgaaaa cagctgttcc cacagttgaa tcagtcgact ctgccctacc
    21721 gccgccagca gctgttttga agcgatagtc gctggcaatt ccgggtaccc caggtgctca
    21781 cgtccggcgg caatcgcacc cgacgtcaca ataacaatcc gatgcccggc ggcatgtaac
    21841 tgcgcgcact ggcgaacaag ttcaacgata tgggcacggt tcaggcggcg cgatccgcct
    21901 gttagcacac tggtgccgag ttttaccacc agcgtctggc tgtcactcat gattctctgc
    21961 cattcaattt taggaaaaaa tgatatcaaa cgaacgtttt agcaggactg tcgtcggttg
    22021 ccaaccatct gcgagcaaag catggcgttt tgttgcgcgg gatcagcaag cgtagcggca
    22081 gttgtttacg cttttattac agatttaata aattaccaca ttttaagaat attattaatc
    22141 tgtaatatat ctttaacaat ctcaggttaa aaactttcct gttttcaacg ggactctccc
    22201 gctgaatatt cgcgcgttaa ttaaaatcag gaatgaaaat gaaaaagagc actctggcat
    22261 tagtggtgat gggcattgtg gcatctgtat ccgtacaggc cgcagaaata tataacaaag
    22321 acggtaataa actggatgtc tatggcaaag ttaaagccat gcattatatg agtgataatg
    22381 acagtaaaga tggcgaccag agttatatcc gttttggttt taaaggcgaa acacaaatta
    22441 acgatcaact gactggttat ggtcgttggg aagctgagtt tgccggtaat aaagcggaga
    22501 gtgatactgc acagcaaaaa acgcgtctcg cttttgccgg gttgaaatat aaagatttgg
    22561 gttctttcga ttatggtcgt aacctgggcg cgttgtatga cgtggaagcc tggaccgata
    22621 tgttcccgga atttggtggc gactcctcgg cgcagaccga caactttatg accaaacgcg
    22681 ccagcggtct ggcgacgtat cggaacaccg acttcttcgg cgttatcgat ggcctgaact
    22741 taaccctgca atatcaaggg aaaaacgaaa accgcgacgt taaaaagcaa aacggcgatg
    22801 gcttcggcac gtcattgaca tatgactttg gtggcagcga tttcgccatc agtggagcct
    22861 ataccaactc tgatcgtacc aacgagcaga acctgcaaag ccgtggcaca ggcaagcgtg
    22921 cagaagcatg ggcaacaggt ctgaaatacg atgccaataa tatttatctg gcaactttct
    22981 attctgaaac acgtaaaatg acgccaatct ctggcggctt tgccaataag acgcagaact
    23041 ttgaagcggt cgctcaatac cagtttgact ttggtctgcg tccatcgctg ggttatgtct
    23101 tatcgaaagg gaaagatatt gagggcatcg gtgatgaaga tctggtcaat tatgtcgatg
    23161 ttggtgctac gtattatttc aacaaaaata tgtcagcgtt tgttgattat aaaatcaacc
    23221 aactggatag cgataacaaa ttgaatatta ataatgatga tattgtcgcg gttggcatga
    23281 cctatcagtt ttaatggata ttgccggatg tgatgcatcc ggcacatttc attcacgccg
    23341 ttaacttcac cggttcatca cgaaaatcat ctgccggttc cagcttcaga ttcagcgtcg
    23401 ccagcagctc acgcagcttc tcgtgaaatt cacgcagggt gtgctccaat cgttcaacca
    23461 cttcggcatc ttttatccga acactcgtcc agtcgcctgc tttatcgaac agaccaaact
    23521 ggtaactgta ggtaaaacgg gattcctgcg cttccagttc catccaccag ccccagaact
    23581 cacgcacttc cggtgccggt ttcacgttga cgcatacagc cagacaatcg aaaaagaatc
    23641 gattatcttc gcacttacct tcacgaatat acgggcctag tgcggtaaat tttttgatca
    23701 atctgctctt cgggtgtcca ctcggtaacg tcattgcaat ctcctgttgt gatgcaactg
    23761 ttttaccaaa ttggtaaaat ttagcaaatt tttaacacaa ccgtttttcg atccagtctg
    23821 tgatttcctg aaggcctttg tcaaaattcc gatacaccgg gttaaatggg atctctaata
    23881 atttaccgtc agcagatgac gacgtgatta agcgtgagtc ctcttccggg ctgaacggat
    23941 cgttcttcca gtagcctgat aacattggcg ttgggcagcg acgtcccagc aatccttgca
    24001 cttttaatga atagcgattc aactccactc gcaacgcttc atcggaagca tcatgcatcc
    24061 ctaaacgact ggccagaacg tcaagataca tttccggcac ctgttgctgg cacttaaaat
    24121 cactcaacag ggtatgaact accggaccaa gacaggcaac cactttcaga cgcggcgatt
    24181 caaggtatgc cagacgcacg gcaacgttag cgccgaaacg gaaaccaaag gccgcgacgc
    24241 gagtgtgatc cacccacggt acgttaggca gcgcctttaa aacgtgctga tgcaacaggc
    24301 tggagtcctg ggtgagcttc cattttgaag aaaagcccac cgacggcata tcaatagtca
    24361 gcatagcaat gccgcgcggc gcaaaataac gttcatacag gctgtaatag tccgtctgca
    24421 tcgcatccag accaccacac attaatactg tcgggaacgg gccatcgcct ttcggcatat
    24481 gcaaaaagcc ggtgatgggc gcaccgccgg gtacggtaaa ctccatctgc cgcatcgtgc
    24541 ccggcagacg ctgagcggcc tcttcatagg cgcggtttga caaagcctgc gcttgctcgg
    24601 ccaggtcatc tcctttcaga tgaggatagg cagcaatgtt gtacaacgta gccgcatgca
    24661 gccagtgtcg accgctgagt tgcggatctt cttccgcaca ggctttttgt tgccacacca
    24721 tcgcctgggt agcccactca taaatccagt tgccgccgcg gtagccaatc accgtatcgt
    24781 ataaatcatc gtcggtacgt tcggcatcgc tcatcacaat acgcgcctgg acgtcgagga
    24841 tttcacgggg gtcaatgccg cgccagatcc acatcagacg gttaatcatg cgataccagt
    24901 gagggatggt tttaccatca agggccgact gcacaggcgg ttgtgcgccg tgactaaagc
    24961 ggcggactag cgtcgaggtt tcaggatgtt taaagcgggg tttgaacagg gtttcgctca
    25021 ggtttgcctg tgtcatggat gcagcctcca gaatacttac tggaaactat tgtaacccgc
    25081 ctgaagttaa aaagaacaac gcccggcagt gccaggcgtt gaaaagatta gcgaccggag
    25141 attggcggga cgaatacgac gcccatatcc cacggctgtt cgatccaggt attttgcggg
    25201 atatcaacaa cataatcatc aaccagcgga cgaccagccg gttttgcgaa gatggtgaca
    25261 aagtgcgctt ttggatacat ttcacgaatc gcaaccgcag taccaccggt atctaccagg
    25321 tcatcaataa cgatgaagcc ttcgccatcg ccttctgcgc gtttcagcac tttaagctcg
    25381 cgctggttgt cgtgatcgta gctggaaata caaacggtat cgacatgacg aatacccagt
    25441 tcacgcgcca gtaacgcacc cggtaccaga ccgccacggc ttacggcaat aatgcctttc
    25501 cattgttcag aaggcatcag tcggcttgcg agtttacgtg catggatctg caacatgtcc
    25561 caggtgacga tgtatttttc gctcatgtga agtgtcccag cctgtttatc tacggcttaa
    25621 aaagtgttcg aggggaaaat aggttgcgcg agattataga gatctggcgc actaaaaacc
    25681 agtatttcac atgagtccgc gtctttttac gcactgcctc tccctgacgc gggataaagt
    25741 ggtattctca aacatatctc gcaagcctgt cttgtgttga caacattttc tgctaaccct
    25801 gtgacctgca atactgtttt gcgggtgatc gacaaggaga cttaacgtgt ctgaactgtc
    25861 tcaattatct ccacagccgc tgtgggatat ttttgccaaa atctgttcta ttcctcaccc
    25921 gtcctatcat gaagagcaac tcgctgaata cattgttggt tgggcaaaag agaaaggttt
    25981 ccatgtcgaa cgcgatcagg taggtaatat cctgattcgt aaacctgcta ccgcaggtat
    26041 ggaaaatcgt aaaccggtcg tcttgcaggc ccacctcgat atggtgccgc aaaaaaacaa
    26101 cgacaccgtg catgacttca ctaaagatcc tatccagcct tatattgatg gcgaatgggt
    26161 taaagcacgc ggcaccacgc tgggtgcgga taacggcatt ggtatggcct ctgcactggc
    26221 ggttctggct gacgaaaacg tggttcacgg cccgctggaa gtgctgctga ccatgaccga
    26281 agaagccggt atggacggtg cttttggctt acagagcaac tggttgcagg ctgatattct
    26341 gattaacacc gactccgaag aagaaggtga aatctacatg ggttgtgcgg gaggtatcga
    26401 cttcacctcc aacctgcatt tagatcgtga agcggttcca gctggttttg aaaccttcaa
    26461 gttaacctta aaaggtctga aaggcggtca ctccggcggt gaaatccacg ttggcctggg
    26521 taatgccaac aaactgctgg tgcgcttcct ggcgggtcat gcagaagaac tggacctgcg
    26581 ccttattgat ttcaacggcg gcacactgcg taacgccatc ccgcgtgaag cctttgcgac
    26641 cattgctgtc gcagctgata aagtcgacgc cctgaaatct ctggtggata cctatcagga
    26701 gatcctgaaa aacgaactgg cagaaaaaga gaagaatctg gcattgctgc tggactccgt
    26761 agcgaacgac aaagctgcat taacggcgac atctcgcgat acctttattc gtctgctgaa
    26821 cgcaaccccg aacggtgtga tccgcaactc cgacgtggca aaaggtgtgg tcgaaacctc
    26881 cctgaacgtc ggtgtggtga ccatgaccga caataacgta gaaattcact gcctgatccg
    26941 ttctctgatc gacagcggta aagactacgt agtgagcatg ctggattcac tgggtaaact
    27001 ggcaggtgcg aaaacagaag caaaaggcgc atatcctggc tggcagccgg acgctaactc
    27061 tccggtaatg catctggtac gtgaaaccta tcagcgtctg ttcaacaaga cgccgaacat
    27121 ccagattatc cacgcgggcc tggaatgtgg tctgttcaaa aaaccgtatc cggaaatgga
    27181 catggtgtct atcgggccaa ccatcaccgg tccacactct ccggatgagc aagttcatat
    27241 cgaaagcgtt ggccattact ggacactgct gactgaactg ctgaaagaaa ttccggcgaa
    27301 gtaattattt gatttgctgc cggatggcgt ttaatcgcct tccggcagtt tcatccttca
    27361 ttacccttcg ataaaggcca tccctgtaaa cgttcgtcgc gggttgccac gttcaatctg
    27421 gtgatggaac attcgccgct gcgatttcag cgccgcgcta ttttcctgtt gctgttgttc
    27481 cagcttccag gcaatcagta atcgcgccag ccgtttgtta gcatgctgac tgcgctctga
    27541 ctgaaccttc acgctaatac cggatgccag atgcgtggcg cgtaccgccg agtcggtttt
    27601 attgacatgt tgaccgcccg gccccgacga acgcagcgtc tcataacgga ttgcatccga
    27661 ttgttcctgc tcatcagcgg taaaacgccc aatgcccaga aaccagtttt tgcgcccatg
    27721 atgaggccga tacggactcg gacaaatcca ctgaatagtg ccgcaccagc tttcgcttag
    27781 agcccatgcg ttatcgccat ccagagaaat cagcgccgaa cgcagtgtgt cagagtagcg
    27841 acccgtttct gtttccagca ccgttaccgc gacgtcctgt cgggtagctt ctttaatcag
    27901 cctgtccagt gcttttctca ctgcgagaca acattcttcc ggcccctgag cagaggagag
    27961 ttgtagcaag atcatccttt tttccctcca ctgtttttga gcgtcagcac cgggcgcagt
    28021 cgcgcgacag gaataattaa cccagccagc accagacatt gcaccacgct ttcagccgat
    28081 ttataggctt gtggcgcttc ttcaaagatg agttgtttat cgcgacaaat tacccgactg
    28141 ccaagttcag tccgcgagag ttgcgtcgcc gtgtatttcg ctgccagacg ccctttacac
    28201 tcggtgcgcc cccatttacg ccccgcccca tgcgccagcg agtggagcgt tttttcgttc
    28261 gcaacaggtt taaccagcca ggagtaatcg ccgcgtgaac cgggaataat caccagaccg
    28321 ttgtcatccg gcgtggcgcc tttacgatgc aaccagccct gttgatcgcc gatttggcac
    28381 gcgctaacaa agttatgcgc cacatccaga accggacttc cggtggcctt aacttgttgc
    28441 attatgcgca aagcgatcag ctggcgatta atacgcgcaa acgccagcgc atcgtcatgt
    28501 tccgcaatat accgtagcgc gtcgtcactg ccttcaggca aaccatgatg cgaaaacgag
    28561 gcaatatgcc gctgtaaaat agactgcccc aatcccctcg agccgctatg aaccagcagt
    28621 tgcagatgct gcgcatccag accagccagt gcaaacagtt ccgcgtcgat aatttgatca
    28681 atctgttgca gttctacgaa gtggttaccg ccaccgatgg agccaagcga gttgcgccac
    28741 ggatgctgtg caaatgctga cggcaggttt tcctccagcc agctttcttc agcaacgtca
    28801 tccagatctg ataatcgctt ttcaaactta tcggcgttgt atttgcgagc gagaatatct
    28861 gtttgccata gcgccatgcc gcagccgata tcattgccga ccagtgccgg gtaaaaacga
    28921 ccaacagaga agaacgctgc gccaatcggg tagccgcgtc cgggatgtaa atctggcatc
    28981 cccacaacgc gctgcatgtt gggtaaattt gccgtggtgt gtaattgttg gatcgctaaa
    29041 ctctcgatcc acaggtcatc agatgcgatg gtaaataccg catcggataa gggacgaata
    29101 tatttgccca tgtaccattc catttatgaa taaaaatcag gaaatggcag acgaatccag
    29161 ggcaagaaaa aagaattaat cctggaaagg tctgcaaaga gtcattgata aaggatttgt
    29221 catgatttac ctccttgcag tttgtggaat aagaaaacgc gcacatacta gacgggcgtt
    29281 attttcattg caagctggat ttaatgttgc ggtttatatc gcatataaaa attagtaaac
    29341 cacgttcccc ggcattcaac gcgctgctgc ttaactgtct gaaaaccata acgttcaaaa
    29401 aagggttttg cggttatgct ggcgtccacc gtaagttcgg attcagactt aatcaatggt
    29461 tttaacaaag cgctggcaac tccgcgtcgg gtgtattccg gttcaacaaa taacatatcg
    29521 atataatgtc caatgcgggt aataaaacca accggttgtg cattaataac tgcaacccgc
    29581 acttgtgatt tcgcgagttt ctccttccag cgagattcgt caatctgcgc ccaggcggca
    29641 atttgctgtg gagaatagtg ctgactcgcc gtcatcgtaa cagctcgaag gaaaatagcg
    29701 catagttgct gaaaatcacc aggctgatag tttcttattt gtatgttatt cataatccca
    29761 gcaccagttg tctttccatt tgagggtcaa gcaacgtcac atgcagcccc accagtcgca
    29821 caccgcgccc gccgcgacgt tcatcccagg ttttacgcgc ggtggcgatt agatcagctt
    29881 tattcagccg cggccagacg tgctcctgag tagtttgctg aaaatcgtcg aactttaatt
    29941 tcaccccctg gcgagcaatc agtaaatcag gttttacctt tgccagacgg cgttcaagtt
    30001 caggatacag ccgctcaata atcgcttcac attcagacca gtgatgaata tcttccgcca
    30061 tcgtgcgttc cacgccgacg gattttcgca accgttcact gttaacgtcg cgctcgtcaa
    30121 tcccctgact acgctcccac aaaatgcggc caaatttgcc aaagcgttta agcagaatca
    30181 ccagatcaca cttttgcaca tcaccgcagg tccgcagccc catcgcttcc agttttgccg
    30241 ccgagacttt gccgacgccg ggaatttttg ccagcggtaa ggtttgcaga aatgccggaa
    30301 cttctgccgg cgtaatcaca aactgaccgt tgggtttatt catgtcggaa gcaattttgg
    30361 cgagaaactt gaccggcgca acgcccgcag acgccgtcag ttgcagctcg ttgaagatcg
    30421 tctggcggat ttcctgggcg atgagggtcg cagaaccgtg gcaatggacg ctatcggtga
    30481 catcgagata ggcttcatcc agtgataacg gttcaatgcg cgaggtatag cgcgagaaaa
    30541 tttcgcggat atgatttgag gcttctttgt aggcgtcaaa gcgccctgga agcaaggtga
    30601 gatgcgggca taatttgagc gccatccctg tcggcatagc gctacgtacg ccaaatttac
    30661 gcgcgggata attggcggtg ctgatcaccc cccgtcgttc gcggctgccg ccaatagcaa
    30721 tagggatatc gcgcagggcg ggattatcgc gcatctccac cgctgcgaaa aagcagtcca
    30781 tatccacatg aatgatttta cgcattgctc acctctcaac actggtaaag tatacagtaa
    30841 tttcaggggt tgagaaatgc gtaaagattc agcatttaac gcgctcgctg cgaaagcacc
    30901 tgctgcttat ccagcttttg caccagcggc tgcaccactt tatccccatg ctgaccaaag
    30961 tattgataca gcgtatcgga cgcacttttg gtcagcacca taatctcaat gcgccggttg
    31021 cccgcgcttt gcggattttt ggcatccagc aacatctggt ccgccattgc gcttacctgc
    31081 atcactttat cttccggcat tccggcctct tccagcaccc gacgagccga aagcgcgcga
    31141 tcgcccgaaa ggttccagtt gttgtagata ttgtttttgt aggccatcgc atcggtatgc
    31201 ccggtaataa taattttgtt atcgagagag tcgaacaccg gcgcaagctc caccagcagc
    31261 gttttaaaga acggcataat cttcgcgctg ccgcgttcaa acatattgcg gttctggtcg
    31321 tctttaatca gcacgcgtaa tccctgggga acaatctcca tctccagatt cgcttccata
    31381 tgcgcatcac gggcgatggt attgatactg gttgccagct cgccaagctc ggtggcggat
    31441 ttcttcttca gcagcgccgt tttctcgttg acgtcgcgcg cttttttctc cgtctcttcc
    31501 ggcaccgcca cggtggcagg ttttggcgga tgagacgggc tgattttatt taacggcgac
    31561 agcccgccac cattgaaaat cgactgcccg tgcagcgcgg cgataatgct ttcacgctcg
    31621 gatttactga cgctgttgac aatcaacttt atcgtcgtca ccaagggggc cgagcgtatt
    31681 tccgaggttt ctgcccgctt taccctggac gcgatgcccg gcaaacagat ggcgattgac
    31741 gccgatctta acgccgggtt gatcaaccag acgcaggcgc aaacccggcg taaagatgtc
    31801 gccagcgagg ccgatttcta cggcgcaatg gacggggcat caaaatttgt gcgcggggac
    31861 gccatcgccg ggatgatgat tctggcaatc aacctgatcg gcggcgtctg tatcggcatc
    31921 ttcaaataca acctgagcgc cgacgccgcc ttccagcaat atgtgctgat gactatcggc
    31981 gatgggctgg tggcgcaaat cccttccctg ctgctctcca ccgcggcggc gattatcgtc
    32041 acccgcgtca gcgataacgg tgatatcgcc catgacgtgc gtaatcagtt gctggcaagc
    32101 ccgtcggttc tctacaccgc cactggaatt atgttcgtgc tggcggtggt gccgggaatg
    32161 ccgcatttcc cgttcctgat gttcagcgcc ctgcttggtt ttaccggctg gcggatgagc
    32221 aagcgcccgc aagcggcgga ggcggaagag aaaagcctcg aaacgctaac ccgtacgatg
    32281 acagaaacca gcgagcaaca ggtcagttgg gaaactattc cgctgatcga acctatcagt
    32341 ttaagcctcg gctacaaact ggtggcgctg gtggataaag cgcagggcaa cccgctcacc
    32401 cagcgtattc gcggcgtacg tcaggtgatt tccgacggca acggcgtgct gctgccggag
    32461 atccgtattc gggaaaactt ccgcctcaag cccagccagt acgccatttt catcaacggc
    32521 attaaggctg atgaagcgga tattccggca gataaactga tggccctgcc ctccagcgaa
    32581 acctacggcg agatagacgg cgtgctgggg aacgacccgg cgtacgggat gccggtcacc
    32641 tggattcagc ccgcgcagaa agcgaaggcg ctgaatatgg ggtatcaggt gatcgacagc
    32701 gccagcgtaa ttgccaccca tgtgaataag attgtgcgca gctatattcc tgatttgttt
    32761 aactatgacg atattactca gctgcataac cgtttgtcgt cgatggcacc gcgactggcg
    32821 gaagatttga gcgcggcgct caattacagc cagttgctga aagtgtaccg ggcgctactg
    32881 actgaaggcg tttccctgcg cgatatcgtc accatcgcca ccgtgttggt cgccagtagt
    32941 gcggtgacta aagatcatat tctgctggcg gccgatgtgc gcctggcgct gcggcgcagc
    33001 attacccatc cgttcgttcg caagcaggag ctgacggtgt atacgctgaa taatgagctg
    33061 gaaaatctgc tgaccaacgt ggtgaatcag gcgcaacagg ccggcaaagt gatgcttgat
    33121 agcgtgccag tggatccgaa tatgctcaac cagttccaga gcacgatgcc tcaggtgaag
    33181 gagcagatga aagcggcggg gaaagatccg gtgctgctgg tgccgccgca gctgcgcccc
    33241 ttgctggcgc gctacgcaag gttgtttgcg ccggggctgc atgtgctgtc gtataacgaa
    33301 gtgccggatg agctggagtt gaagataatg ggggcgctga tataggcatg ataagacgcg
    33361 tctgcgtcgc atctggcacc ggtacacaac cgccggatgc ggcgtgaacg ccttatccgg
    33421 cctacgggtg agcacggttt tgtcggcatg ataagacgcg tggcgtcgca tctggcatcg
    33481 gcgcacaacc gccggatgcg gcgtgaacgc cttatccggc ctactggtga gtgcggtttt
    33541 gtcggcatga taagacgcgt agtgtcgcat cagacaccgg cgcacaaccg ccagatgcgg
    33601 cgtgaacgcc ttatccggcc tacaaaatcg tctcttccgt caaaatccgt cgcgcgccta
    33661 aaaaatggtc ctgccagaaa ggttcggcta accgacttac ccgaatggtt tccccggtgc
    33721 gcggtgactc gataaactga ccgtctccaa gatatacgcc catatggtcg gctatctcac
    33781 gactgtggat atggaaaaac agcaaatctc cccggcgcag gtcgttattc gccacaatcg
    33841 ttgcccgacg gtagtggtac atctcattgg ccgtgcgcgg gagcttagcc tcaaggatct
    33901 tgttgtaggc ataaaaaacc aacccgctac agtcgaagcc tttatcaggc cgcgtgcccc
    33961 cccagatata aggcttacct agctgctgct ccaggcggtg aatcgccact tcggtgatgt
    34021 tatgtagatg gtcgctgctt aagaaatgat tattctccac caacgcctgc cagcgtctgt
    34081 cactcgcctt gagcggcgca ggaaaccatt ctggatgctg ttttatctgc tcccgattat
    34141 gctggcgcag cgcccttctg cttttggcat tgccttccac aatatagctg gcctgctttt
    34201 tcagatgggt ttggtactgt tttaataaac gcgcacggtt ttgcatccgc tggcgggcgg
    34261 cgtagctaaa agagatatgt gatgtggtgg cgctggcgag taaagggagc aaaacgcata
    34321 acgaaaacac gcctggatgc agaaatctgc cgtgcaagaa cgaagacatg aaggacataa
    34381 accagctgaa tgaaggtgag aattttaaaa gaccggagga gtaaacggga ttattaattg
    34441 cttatttgca tattactatt gcggtttcat aatcttagga aataagaaat aaatctacaa
    34501 tccatgcgct tttcggactc ttgaaaactg cccctgtgct aaatttgtaa accctgcgcc
    34561 gacaggcttg cgtaaaactc ttttggtgat aatgtgagcc ttccaaatcc agactattcc
    34621 gatttgggat tcattttgcc gtcctggcgt gtgtaaccgt tttatcaagg aataagcagt
    34681 atgcgtaaaa tcgcattaat tcttgcgatg ttgttgatcc cgtgcgtttc gtttgccggt
    34741 ttgctgggca gcagtagctc gaccacgcca gtgagcaaag agtataagca gcagttgatg
    34801 ggatcccctg tctacatcca gatcttcaag gaagaacgta cgctcgatct ctacgtcaaa
    34861 atgggcgagc aatatcaact gctcgacagc tataaaatct gtaaatattc cggcggatta
    34921 gggccaaaac agcgtcaggg cgatttcaaa agcccggaag ggttttatag cgtccagcgt
    34981 aatcagttaa aaccagacag ccgttactac aaagcgatta atattggatt cccaaacgcc
    35041 tatgaccgtg cgcatggtta tgaagggaaa tacctgatga ttcacggcga ttgtgtttcc
    35101 atcggctgct acgcgatgac caatcagggt attgatgaga tcttccagtt cgttactggt
    35161 gcgctggtgt ttggtcagcc gagcgtgcaa gtgagtattt acccgttccg tatgaccgac
    35221 gccaatatga agcgccataa atattccaac tttaaggact tctgggagca actgaagccg
    35281 ggctacgact actttgagca aacccgtaag ccaccgaccg tttctgtggt caatggccgt
    35341 tacgtggtca gcaagccgtt aagccacgaa gttgtgcagc cacaactggc atcaaactac
    35401 acgctccccg aggaaaaata agcgccattc acctggcata atcttttgcc aggtttcatt
    35461 gcccgtcagc ggctgtgtcg caatcaccgt gaccacatca ttcggtgtgg tctgcgagct
    35521 gaagtcgatt tccacatcct gatccagcaa cgttgccacg ccaaacggcg cgcggcgggt
    35581 gatccagtgt aaattagtcg agcaatacgc cattacatag cgcccgtcag aaagcagcat
    35641 gttgaaaacg cccttctgcc gcagttcatc cgccagtgag gcgatatatt taaacactgc
    35701 cgccatgttg cccggtgtgc gcgggtaacg ctgcgttaat ttatgcagga gccagcaaaa
    35761 ggctttttcg ctatcggttt caccgaccgg gcggaagttg ccggtttcca gtgatttgta
    35821 gcccgtcagt tgtccattat gggcataagt ccagttacgc ccccataact cgcgggtaaa
    35881 cgggtgagta ttttccagcg ccacctcgcc ccgattagcc tggcgaatat gagccaccac
    35941 cgaacaggat tttatcgggt agtcctggac aagtttggcg atgggggaat taaagctggg
    36001 ttgcggatct ttaaatgtgc gacagccttt accttcgtaa aaggtaatgc cccagccatc
    36061 tttatgtggc ccggttccac caccacgctg tacaagtccg gtgaaactaa agcagatatc
    36121 ggtagggacg ttggcgctca tcccgagcaa ttcgcacatg ggtaacctcc acaacagcgg
    36181 gagcgcatgg cccccggcga agattgattt aagaattttc aggtcggatg tggcgtgaac
    36241 gcattattcg gcctacggtt cgggtgcaga ttgtaggcat gataagacgc gtcagcgtcg
    36301 catcaggcat cagagcacaa atgccggatg cggcgtaaac gccttatctg gcctacgcca
    36361 gacttactta accatctctt tttcaattaa ctggatcagg atatggatca ctttaatgtg
    36421 gatctcctga atgcggtcgg cataaccaaa gtgcggaacg cgaatttcaa tatccgccgt
    36481 gccagccatt ttgccgccgt ctttacccgt cagggtgatc actttcatcc ccttctcacg
    36541 cgccgctgcg atcgctttga tcacgtttgc agagttaccg gaggtggaga tccccagcag
    36601 tacatcgcct tcgcgaccta ccgcttcaac gtaacgcgag aaaatatcat tgaaaccaaa
    36661 atcgttaccg acgcaggaaa tatgactaac gtcagaaatg gcaatcgccg ggtagcccgg
    36721 acggttttca cggtagcgac cggtcaactc ttcggcaaag tgcatggcgt cgcaatggga
    36781 gccgccgttg ccacaggaaa gcactttacc accggcttta aagctgtctg ctaacaggac
    36841 cgccgcgcgc tgaatggcgt gaatattggc gtcatctttt aaaaagttag ccagcgtttc
    36901 cgccgcttcg ttcagttcat tacgaataag atcctggtac atgaggatat ccttcagcat
    36961 aaatgtaata gacaaaatgc agtgtaccgg ataccgccaa aagcgagaag tacgggcagg
    37021 tgctatgacc aggacttttt gacctgaagt gcggataaaa acagcaacaa tgtgagcttt
    37081 gttgtaatta tattgtaaac acattgctaa atgtttttac atccactaca accatatcat
    37141 cacaagtggt cagacctcct acaagtaagg ggcttttcgt tatgatgatt ttgagtattc
    37201 tcgctacggt tgtcctgctc ggcgcattgt tctatcaccg cgtgagctta tttatcagca
    37261 gtctgatttt gctcgcctgg acagccgtcc tcggcgttgc tggtctgtgg tcggcgtggg
    37321 tactagtgcc tctggccatt atcctcatac cgtttaactt tgcccctatg cgtaaatcga
    37381 tgatttctgc gccagtgttt cgtggattcc gcaaagtgat gccgccgatg tcgcgcaccg
    37441 agaaagaagc gatcgacgcg ggcaccacct ggtgggaggg cgacttgttc cagggcaagc
    37501 cggactggaa aaagctgcat aactatccgc agccgcgcct gaccactgaa gagcaagcgt
    37561 ttctcgacgg tccggtagaa gaagcctgcc ggatggcgaa tgatttccag attactcatg
    37621 agctggcgga cctgccgccg gagttgtggg cgtaccttaa agagcatagt ttcttcgcga
    37681 tgatcatcaa aaaagagtac ggcgggctgg agttctcggc ttatgcccag tctcgcgtgc
    37741 tgcaaaaact ctccggcgtg agcgggatcc tggcgattac cgtcggcgtg ccgaactcat
    37801 taggcccggg cgagctgctg caacattacg gcactgacga gcagaaaaat cactatctgc
    37861 cgcgtctggc gcgtggtcag gagatccctt gctttgcact gaccagcccc gaagcgggtt
    37921 ccgatgcggg cgcgattccg gataccggga ttgtctgcat gggcgaatgg cagggccagc
    37981 aggtgctggg gatgcgtctg acctggaaca aacgctacat tacgctggca ccgattgcga
    38041 ccgtgcttgg tctggcgttt aaactctccg acccggagaa attactcggt ggtgcagaag
    38101 atttaggtat tacctgtgcg ctgatcccga ccaccacgcc gggtgtggaa attggtcgtc
    38161 gccacttccc gctgaacgta ccgttccaga acggaccgac gcgcggtaaa gatgtgttcg
    38221 tgccgatcga ttatatcatc ggcgggccga aaatggccgg gcaaggctgg cggatgctgg
    38281 tggagtgcct ctcggtaggc cgcggcatca ccctgccttc caactcaacc ggcggcgtga
    38341 aatcggtagc gctggcaacg ggcgcgtatg cgcacattcg tcggcagttc aaaatctcta
    38401 tcggtaagat ggaagggatt gaagagccgc tggcgcgtat tgccggaaac gcttatgtaa
    38461 tggatgctgc ggcatcgctg attacctacg gcattatgct cggtgaaaaa cctgccgtgc
    38521 tgtcggctat cgttaagtat cactgtaccc accgcgggca gcagtcgatt attgatgcga
    38581 tggatatcac tggtggtaaa ggcattatgc tcgggcaaag caacttcctg gcccgtgctt
    38641 accagggcgc accgattgcc atcaccgttg aaggggccaa cattctgacc cgcagcatga
    38701 tgatcttcgg acaaggagcg attcgttgcc atccgtacgt gctggaagag atggaagcgg
    38761 cgaagaacaa tgacgttaac gcgttcgatc aactcctgtt taaacatatc ggtcacgtcg
    38821 gtagcaacaa agttcgcagc ttctggctgg gcctgacgcg cggtttaacc agcagcacac
    38881 caaccggcga tgccactaaa cgctactatc agcacctgaa ccgcctgagc gccaacctcg
    38941 ccctgctttc tgatgtctcg atggtggtgc tgggcggcag cctgaaacgt cgtgagcgta
    39001 tctcggcacg tctgggggat attttaagtc agctttacct cgcctctgcc gttctgaagc
    39061 gttatgacga cgaaggccgt aatgaagccg acctgccgct ggtgcactgg ggtgtacagg
    39121 atgcgctgta tcaggcagaa caggcgatgg acgatttact gcaaaacttc ccgaaccgcg
    39181 tggttgccgg gctgctgaat gtggtgatct tcccgaccgg acgtcattac ctggcaccgt
    39241 ctgacaagct ggatcataaa gtggcgaaga ttttacaagt gccgaatgcc acccgttcac
    39301 gtattggtcg cggtcagtac ctgacactga gcaagcataa cccggttggt ttactggaag
    39361 aggcgctggt ggatgtgatt gccgccgacc cgattcatca gcggatctgt aaagagttgg
    39421 gtaaaaacct gccgtttacc cgtctggatg aactggcgca caacgcactg gcgaaggggc
    39481 tgattgataa agatgaagcc gctattctgg tgaaagctga agaaagccgt ctgcgtagta
    39541 ttaacgttga tgactttgat ccagaagagc tggcgacgaa gccggtaaag ctgccggaga
    39601 aagtgcggaa agttgaagcc gcgtaattga ataacggagc cgaaaggctc cgtttcttta
    39661 tccgctaatt atttaaaatt aaagccatcc ggatggtttt ccaggctgcc ggtcaacgcc
    39721 gcgaacagca ccgttttacc atcaatagaa agcgcatcgt tcacattcaa ccaggtgagt
    39781 ttctcttgcg acgttttctc atcaatggtc gagaacagcc ccgtcatctg attagatttc
    39841 tcggaccaca tcacagcgat acgttgcgag ccacagtcat gcggtttgca cgcgctcatc
    39901 acctgatacg tctcatctcc caacgttacg gtttgtgcgg gagtatacgt accgcctttc
    39961 atcacccagg caggcagctt atgcccttgc accatctgat taaaagcagc tttggtggtt
    40021 tcgccctttg caaggctgct aatggttaaa tcatcctgcg ccattgcact ggtggcgatg
    40081 accagagcgg cgactgtcgt tattgcctta aacatcattc ctcccgggct tatcctgccc
    40141 atgttattaa cctcctaaag cttggcgata acacgataat ttgcaaatca gatatttcca
    40201 atatcagaat ttgttatcaa tttattctgc gctaaggcaa ctgcctgata aggtgttaaa
    40261 gttataaaac ccttcgcata aaggagttgt acaccgtgcc tggtttgaag attacgcttt
    40321 tgcagcaacc actggtgtgg atggatggcc ctgccaatct gcgccatttt gatcgtcaac
    40381 tggaaggtat taccgggcgc gatgtgatcg ttctaccgga aatgtttacc agcggctttg
    40441 ccatggaagc ggcagcttcg tcgctagcac aagatgacgt agtgaactgg atgacagcca
    40501 aagcgcagca gtacaatgcg ctgattgcag gcagtgttgc attacaaacg gagtctggtt
    40561 cggttaaccg ctttttgctg gttgagccgg gcggcacggt acatttttat gataagcgtc
    40621 atctgttccg catggcagat gagcatctac attataaagc gggcaatgcg cgagtgattg
    40681 tggaatggcg cggctggcgt attttgccgc tggtgtgcta cgacttacgt tttccagtgt
    40741 ggtcgcgcaa tctcaacgat tatgacctcg ccctgtacgt cgccaactgg cctgctccgc
    40801 gctctctgca ctggcaggca ttgctgacgg cccgcgcgat tgagaatcag acgtatgtag
    40861 cgggatgcaa tcgcgtcggc agcgatggca acggctgcca ttatcgcggt gacagccggg
    40921 tgattaatcc acagggcgag attatcgcta ctgccgacgc gcatcaggca acgcgcattg
    40981 atgcggagtt gtcgatggct gtattaaggg aatatagaga aaagtttccg gcatggcggg
    41041 atgcggatga gtttaggttg ctgtgagtgg gtgttgcgtt tagggccggg gagagtcggg
    41101 gtaagactac gaaagcccgc tctcctgcga cggcttttat cataggagtg tggaagtgct
    41161 tttccatcaa tcgcgatgac gtctttgtca tctgaagaat gacattcacg catccggtta
    41221 ataaagcatt cgtgaaatta tactgaacgg atacaagaca taactctaat tatagtgccg
    41281 tgaacaggat taccactttc aaaattacga tattattagc agtaattatg ttattaatca
    41341 ttgtgatatt tccatctctg tgatcaatac cggataagca tttaattgca gcatttgcac
    41401 gaaattatcg cagataactg gcaataacaa taacccatca gcccgcttct gtaatacctc
    41461 cattcgttct actgattcaa cattaacaac caaaccaagg gtatttagcg atttaaggat
    41521 ctccagatgt tcactcatcc agcgcagaga aaaaggatca tcaccaatta taaaaacagg
    41581 cccctagcag gttaataact ggcgtttcat tacggatcta ggatgaagtc gagtacatat
    41641 tacaggaaat acgccctgtt gcgatggttc cgatgatgtg gaatattcag aaatatttcc
    41701 attgttagtg aagaattaac atccattaat aatggtgaaa cagcctcact ttccttgcta
    41761 gtaatggctg cactaatctg aaaggaaaat aacaatatta ttacgtataa actcattttt
    41821 aacataatac attcaatccg tttattcatg ttatagaata atgatgctat acccgtgatc
    41881 attgaagatg cttgattttg tcatgacaga ggatcatgct acgcaacatg aaaatcttca
    41941 tctccgatct acaaaaagca gagctggaac gccttcacga taccagtcgt aacaagcggg
    42001 tctgcgatcg tattaaagcc attcttctgg cttctgaagg ctggagttca gtcatgattg
    42061 ctcaggctct gcgcctgcat cagaccaccg ttgatcatca tatccatgag tttatgaata
    42121 aagggaagtt aaagcccgaa aacggtggtt cagacagtaa actcagtgct gaacaaacat
    42181 cactcttaat caaacattta tctgataatt tgtttcacca tactcatgaa attatcgcct
    42241 ttgttctcca cacatgggga attttgttca gcgttgccgg tatgaacaaa tggttgcatc
    42301 gtaacggctt ctcttacaaa aaaccggctg gaacgcctca taaattcagc gaagagaggc
    42361 aggcgcaatt cattgaattt atgaagacct gaaagaaaaa gcaggcgatg aacctgttct
    42421 ttttatagat gctgtccacc ccactcaggc caccaaaatc agttatggct ggatacgaaa
    42481 agggcaccgg aaagcaatag aaacaacagg tagccgtaca cggctgaata tcatgggtgc
    42541 gctaaatctg aagtctccgg aacgcccact catcagtgag tacccaacca ttaatgcgtt
    42601 aagcatatcc cggtttttca atgagataag aaaggtttat cctgattata atcaaacagt
    42661 caacatcatt ctggatggtg cacgttatca ccatgctcaa ctggtaacag actgggctga
    42721 agtcgttaat atcaaacgcg ttcaattagc acttatctac ata
//
LOCUS       DAPHCV010000027        42689 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.027, whole genome shotgun sequence.
ACCESSION   DAPHCV010000027 DAPHCV010000000
VERSION     DAPHCV010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.027"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..456
                     /locus_tag="RH033_001518"
     CDS             <1..456
                     /locus_tag="RH033_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDV2990722.1"
                     /translation="ANHCAEYIEIFDESIAFTCLSAFSSEKQFGVFLFGCLKSTGAKV
                     KTIIHTDLSAPWRLKDISSRLYLSESLLKMKLKEEGVSFSKIILDERMQMAEYLLSTR
                     CYPISKVAKVCGYASVSYFTYVFRRYFGVSPSQYSQRSSESKILTHQGI"
     gene            complement(498..950)
                     /locus_tag="RH033_001519"
                     /pseudo
     CDS             complement(498..950)
                     /locus_tag="RH033_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1014..1160
                     /locus_tag="RH033_001520"
                     /pseudo
     CDS             1014..1160
                     /locus_tag="RH033_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1559..2035)
                     /locus_tag="RH033_001521"
     CDS             complement(1559..2035)
                     /locus_tag="RH033_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HDV2990723.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPVGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(2094..3383)
                     /gene="bioA"
                     /locus_tag="RH033_001522"
     CDS             complement(2094..3383)
                     /gene="bioA"
                     /locus_tag="RH033_001522"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HDV2990724.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERNMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQH
                     QVAAIEAQLREQLAPACDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3470..4510
                     /gene="bioB"
                     /locus_tag="RH033_001523"
     CDS             3470..4510
                     /gene="bioB"
                     /locus_tag="RH033_001523"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDV2990725.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVREAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            4507..5661
                     /gene="bioF"
                     /locus_tag="RH033_001524"
     CDS             4507..5661
                     /gene="bioF"
                     /locus_tag="RH033_001524"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HDV2990726.1"
                     /translation="MIWQEKIDAALDARRVADALRRRYPVAQGAGRWLVADDCQYLNF
                     SSNDYLGLSHHPQIIRAWQQGADQFGVGSGGSGHVSGYSVAHQVLEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSPLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSDTVADYLVQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLAGSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5648..6403
                     /gene="bioC"
                     /locus_tag="RH033_001525"
     CDS             5648..6403
                     /gene="bioC"
                     /locus_tag="RH033_001525"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HDV2990727.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRKRHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIELSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRYPRIL
                     TRSQLQRLQLSWPQQQGRYPLTYHLFLGVIARE"
     gene            6396..7073
                     /gene="bioD"
                     /locus_tag="RH033_001526"
     CDS             6396..7073
                     /gene="bioD"
                     /locus_tag="RH033_001526"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDV2990728.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIRHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7652..9673
                     /gene="uvrB"
                     /locus_tag="RH033_001527"
     CDS             7652..9673
                     /gene="uvrB"
                     /locus_tag="RH033_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HDV2990729.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9865..10773)
                     /gene="yvcK"
                     /locus_tag="RH033_001528"
     CDS             complement(9865..10773)
                     /gene="yvcK"
                     /locus_tag="RH033_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HDV2990730.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAISEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     regulatory      11038..11182
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11170..12159
                     /gene="moaA"
                     /locus_tag="RH033_001529"
     CDS             11170..12159
                     /gene="moaA"
                     /locus_tag="RH033_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDV2990731.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12181..12693
                     /gene="moaB"
                     /locus_tag="RH033_001530"
     CDS             12181..12693
                     /gene="moaB"
                     /locus_tag="RH033_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HDV2990732.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12696..13181
                     /gene="moaC"
                     /locus_tag="RH033_001531"
     CDS             12696..13181
                     /gene="moaC"
                     /locus_tag="RH033_001531"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDV2990733.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13174..13419
                     /gene="moaD"
                     /locus_tag="RH033_001532"
     CDS             13174..13419
                     /gene="moaD"
                     /locus_tag="RH033_001532"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HDV2990734.1"
                     /translation="MINVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNKTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13421..13873
                     /gene="moaE"
                     /locus_tag="RH033_001533"
     CDS             13421..13873
                     /gene="moaE"
                     /locus_tag="RH033_001533"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HDV2990735.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            14010..14714
                     /locus_tag="RH033_001534"
     CDS             14010..14714
                     /locus_tag="RH033_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDV2990736.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14920..15309
                     /locus_tag="RH033_001535"
     CDS             14920..15309
                     /locus_tag="RH033_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2990737.1"
                     /translation="MESYSQSSNKLDFQHEARILNGIWLITALGLVTTAGLALVIIRN
                     AALMSERPIWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVSCAFTGALTLY
                     YYFIGFFGILAAIAITLVWQRHTRFFH"
     gene            complement(15345..16301)
                     /locus_tag="RH033_001536"
     CDS             complement(15345..16301)
                     /locus_tag="RH033_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HDV2990738.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLSGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16301..17542)
                     /gene="clsB"
                     /locus_tag="RH033_001537"
     CDS             complement(16301..17542)
                     /gene="clsB"
                     /locus_tag="RH033_001537"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HDV2990739.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPRRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(17539..18300)
                     /locus_tag="RH033_001538"
     CDS             complement(17539..18300)
                     /locus_tag="RH033_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDV2990740.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLQLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18433..18843
                     /locus_tag="RH033_001539"
     CDS             18433..18843
                     /locus_tag="RH033_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HDV2990741.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18805..19911)
                     /locus_tag="RH033_001540"
     CDS             complement(18805..19911)
                     /locus_tag="RH033_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2990742.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVSLFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(19922..21055)
                     /locus_tag="RH033_001541"
     CDS             complement(19922..21055)
                     /locus_tag="RH033_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2990743.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21048..22784)
                     /locus_tag="RH033_001542"
     CDS             complement(21048..22784)
                     /locus_tag="RH033_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV2990744.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDAALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPTALTQTMSGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPDETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHGNE"
     gene            complement(22777..23772)
                     /gene="hlyD"
                     /locus_tag="RH033_001543"
     CDS             complement(22777..23772)
                     /gene="hlyD"
                     /locus_tag="RH033_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HDV2990745.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23775..24446)
                     /gene="cecR"
                     /locus_tag="RH033_001544"
     CDS             complement(23775..24446)
                     /gene="cecR"
                     /locus_tag="RH033_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HDV2990746.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     SDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24675..26036
                     /gene="rhlE"
                     /locus_tag="RH033_001545"
     CDS             24675..26036
                     /gene="rhlE"
                     /locus_tag="RH033_001545"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HDV2990747.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLLTRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGRGQGGGRGQQQSRRAESGAKSGNAKPAEKPSRRLGDAKPAGEQQRRRRPRKPA
                     AAQ"
     gene            complement(26269..26469)
                     /locus_tag="RH033_001546"
                     /pseudo
     CDS             complement(26269..26469)
                     /locus_tag="RH033_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1768 domain-containing protein"
     gene            26573..28723
                     /gene="dinG"
                     /locus_tag="RH033_001547"
     CDS             26573..28723
                     /gene="dinG"
                     /locus_tag="RH033_001547"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HDV2990748.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRFILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28751..29713
                     /gene="ybiB"
                     /locus_tag="RH033_001548"
     CDS             28751..29713
                     /gene="ybiB"
                     /locus_tag="RH033_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HDV2990749.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLMATGEAATISDGLARVNQAF"
     gene            29854..30939
                     /gene="hcxB"
                     /locus_tag="RH033_001549"
     CDS             29854..30939
                     /gene="hcxB"
                     /locus_tag="RH033_001549"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HDV2990750.1"
                     /translation="MVSGHRFDAQRLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKVVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKARQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDEDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(31168..31428)
                     /locus_tag="RH033_001550"
     CDS             complement(31168..31428)
                     /locus_tag="RH033_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV2990751.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31693..31959)
                     /gene="ybiI"
                     /locus_tag="RH033_001551"
     CDS             complement(31693..31959)
                     /gene="ybiI"
                     /locus_tag="RH033_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HDV2990752.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(32033..32710)
                     /gene="ybiX"
                     /locus_tag="RH033_001552"
     CDS             complement(32033..32710)
                     /gene="ybiX"
                     /locus_tag="RH033_001552"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HDV2990753.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEMLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPESYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSHYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32923..35205)
                     /locus_tag="RH033_001553"
                     /pseudo
     CDS             complement(32923..35205)
                     /locus_tag="RH033_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
     gene            complement(35470..35730)
                     /locus_tag="RH033_001554"
     CDS             complement(35470..35730)
                     /locus_tag="RH033_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV2990754.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTAIIYK"
     gene            36006..36932
                     /gene="rlmF"
                     /locus_tag="RH033_001555"
     CDS             36006..36932
                     /gene="rlmF"
                     /locus_tag="RH033_001555"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HDV2990755.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(36929..39154)
                     /gene="ybiO"
                     /locus_tag="RH033_001556"
     CDS             complement(36929..39154)
                     /gene="ybiO"
                     /locus_tag="RH033_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HDV2990756.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIHDETARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTIWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLVIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     L"
     gene            complement(39271..39993)
                     /gene="glnQ"
                     /locus_tag="RH033_001557"
     CDS             complement(39271..39993)
                     /gene="glnQ"
                     /locus_tag="RH033_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HDV2990757.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(39990..40649)
                     /gene="glnP"
                     /locus_tag="RH033_001558"
     CDS             complement(39990..40649)
                     /gene="glnP"
                     /locus_tag="RH033_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HDV2990758.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(40788..41534)
                     /gene="glnH"
                     /locus_tag="RH033_001559"
     CDS             complement(40788..41534)
                     /gene="glnH"
                     /locus_tag="RH033_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HDV2990759.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(41938..42441)
                     /gene="dps"
                     /locus_tag="RH033_001560"
                     /gene_synonym="pexB"
     CDS             complement(41938..42441)
                     /gene="dps"
                     /locus_tag="RH033_001560"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HDV2990760.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
BASE COUNT        10249 a        10986 c        11339 g        10115 t
ORIGIN      
        1 gctaatcact gtgctgaata catagaaatt tttgatgagt ctattgcttt tacatgtctc
       61 tctgcatttt cttctgaaaa acaatttgga gtatttctgt ttggatgttt gaaaagcaca
      121 ggggctaaag ttaaaacgat tattcatacg gatttatctg caccatggcg tcttaaggat
      181 atatcatcaa gattatatct cagcgaaagt ttactaaaga tgaaattgaa agaagagggg
      241 gtatcattca gtaagatcat acttgatgag aggatgcaaa tggctgaata tttactcagc
      301 actcgttgtt atcctattag taaagtagct aaggtctgtg gttatgccag tgtctcatac
      361 tttacttatg tatttagacg ttattttggt gtttctccaa gtcaatactc tcagaggagt
      421 tcagaaagta aaattcttac tcaccaggga atctgatcat tgttcttgcc cccttatttc
      481 cagacagggg gtgtatctta agttaacgtt acccgctgac gtcgatattc tcgcggagag
      541 cgataaccca gcgcactgtg cggatggttt tcattgtaat gttcgatcgc cactgcaaga
      601 ttatgcaatg ccgttcttac attcggtttc ggcatgaacg cgatgtagtc ttccttcatc
      661 gttttcacga acctttctgc cattccatta ctctgaggac tgctgattgc cgttgtgcag
      721 ggctccagat tcaactctct ggcgaactgc cgtgtttcat gcgctctata cgctgaaccg
      781 ttatctgtca gccactggat ggactgttcc ggtactttat cgccaaagcg tttttctatc
      841 gctcctaaca tgacatcctg cactgtcgct ttatcgtaac ctcccgtgct tgcggcccag
      901 tctatggctt cacgatcgca acagtccagg gcgaacgtaa cacgcagttt tgaatcgcca
      961 cgggtttaac agacacctca gagtcattta agatggctta aagagaggtg cccatgagcg
     1021 gtaagcgtta tcccgaagag tttaaaactg aagcagtcaa acaggttatt gatcgcggtt
     1081 attctgttgc cagcgttgca acacgtctcg atatcaccac ccacagcctt tatgcctgga
     1141 taaagaagta cggtccggat tactccactt tttcatcaag ccaatccgcc caccactgca
     1201 tcatttctct gcgcttatcg agatactgag cgtggttgta aattcagcta acccagtgtg
     1261 ttgacaaaaa attagcgcaa gaagacaaaa aaatcacctt gcgctaatgc tctgttacag
     1321 gtcactaata ccatctaagt ggttgattca tagtgactgg atatgttgtg ttttgtagca
     1381 ttatgcagtc tattttttag actgaatcaa ttataagccg ttgatattaa ttgtttttac
     1441 cgtttcacat tcaacttttt tatactaact ttagcgaaac gggaaggtaa aaagacaaaa
     1501 agttgttttt aataccttta agtgatacca gatggcattg cgccatctgg cagagtgatt
     1561 aactaaacat cgcagtaatc gaggcgcttg ccagagagtg gaaatgaacg ttaaacccga
     1621 ccatcgcgcc gctggcacct tcatcgacat caatacgttc tacatccagc gcgtgaacgg
     1681 taaaaatgta gcgatgggtt tcgcctttcg gcggcgctgc accatcgtac ccggttttac
     1741 caaagtcggt acgcgtctgc aaaacgccgt ctggcattgc taccagacca gagccaaacc
     1801 cttgcggtaa tacgcgggta tcagcgggta aattaacaac tacccagtgc caccagccgg
     1861 agccggttgg cgcatccggg tcatagcagg tgacaacaaa acttttcgtt cccacaggaa
     1921 catcatccca cgccagatgc ggtgaaatat tatcgccatc gtaacccatg ccgttaaaaa
     1981 catgacgatg cggcagctta tcgccatcgc gcagatcgtt actgatgagt ttcatgaacc
     2041 ctcctttctt gtttgcagaa agtgtagcca gaaaccctca cgctgacttc ccgttattgg
     2101 caaaaaaatg tttcatcctg caccgcacgg ttaaccgctg cggtcagacg ctgcaactgt
     2161 tgcgggagaa taatataggg cggcatcagg taaatcagtt tgccaaaagg ccggatccat
     2221 accccctgtt cgacaaagaa tttttgcagc gctgccatat tcaccggacg agtggtttcg
     2281 accacgccga ttgcccccag tacgcgcaca tcggcaacca tttcggcgtc acaggcgggg
     2341 gcaagttgct cgcgtagctg cgcttcaata gccgccacct gatgctgcca gtcgccagat
     2401 tcaagaatcg ccaggctggc gtttgctgct gcgcaggcca gcggattgcc cataaaagtt
     2461 ggcccatgca taaagcagcc ggcttcgccg ttactgatgg tttccgcaac ctcgcgcgtg
     2521 gtgagcgtgg cggaaagggt cattgtgccg ccggttaagg ctttaccgag acacaaaata
     2581 tccggcgcga tttctgcatg ttcacaggca aacagtttgc cggtacgacc aaatccggtg
     2641 gcgatctcgt cggcaatcag caagatacct tcgcgatcgc acattttgcg gattcgtttt
     2701 aaccattccg gatggtacat gcgcatcccg cctgcgccct ggacaatcgg ttcaatgatc
     2761 accgccgcga tttcatgacg atgcgctgcc atcaggcggg caaagcccac catattgcgc
     2821 tcatcccatt cgccatccat gcggctttgc ggggcgggag caaacaggtt ttctggcaga
     2881 tagcctttcc acaggctgtg cattgagttg tccggatcgc acaccgacat cgcgccaaag
     2941 gtatcgccat gataaccatt gcggaaggtt agaaaacgct ggcgcgcttc gcctttggcc
     3001 tgccagtact gcaacgccat tttcatcgcc acttctaccg ctacggagcc ggagtccgcg
     3061 agaaaaacgc actccagcgg ttgcggcgtc attgccacca atttgcggca cagctcaatg
     3121 gcgggcgcat gggtgatacc gccaaacatc acatgcgaca tagcatcaat ttgcgacttc
     3181 atcgccgcat taagctgcgg gtgattgtaa ccgtggatcg ccgcccacca ggacgacata
     3241 ccgtcaacca ggcgcctgcc gtcagacaaa attagctcgc aaccttcggc gcttgccacc
     3301 ggataaaccg gcagcgggga ggtcatggat gtgtatgggt gccagatatg gcgttggtca
     3361 aaggcaagat cgtccgttgt cataatcgac ttgtaaacca aattgaaaag atttaggttt
     3421 acgagtctac accgaattaa caacaaaaaa cacgttttgg agaagcccca tggctcaccg
     3481 cccacgctgg acattgtcgc aagtcacaga attatttgaa aaaccgttgc tggatctgct
     3541 gtttgaagcg cagcaggtgc atcgtcagca tttcgatcct cgtcaggtgc aggtcagcac
     3601 tttgctgtcg attaagaccg gagcttgtcc ggaagattgc aaatattgcc cgcaaagctc
     3661 gcgctacaaa accgggctgg aagccgagcg gttgatggaa gttgaacagg tgctggagtc
     3721 ggcgcgcaaa gcgaaagcgg cgggatcgac gcgtttctgt atgggcgcgg cgtggaagaa
     3781 tccccacgaa cgcgatatgc cgtacctgga acaaatggtg cagggggtaa aagcgatggg
     3841 tctggaggcg tgtatgacgc tgggcacgtt gagtgaatct caagcgcagc gcctggcgaa
     3901 tgccgggctg gattactata accacaacct cgacacctcg ccggagtttt acggcaatat
     3961 catcaccacc cgcacttatc aggaacgtct cgatacgctg gaaaaagtgc gagaagctgg
     4021 gattaaagtc tgttcaggtg gcattgtggg cttaggcgaa acggtgaaag atcgcgccgg
     4081 attattactg caactggcaa acctgccgac gccgccggaa agcgtgccaa tcaacatgct
     4141 ggtgaaggtg aaaggcacac cgctggcgga taacgatgat gtcgatgcct ttgattttat
     4201 tcgcaccatt gccgtagcgc ggatcatgat gccgacctct tacgtacgcc tttctgccgg
     4261 acgcgagcag atgaacgaac agactcaggc gatgtgcttt atggctggcg caaactcgat
     4321 tttctacggt tgcaaactgc tgactacgcc gaatccggaa gaagataaag acctgcaact
     4381 gttccgcaaa ctggggctaa atccgcagca aactgccgtg ctggcgggcg ataacgaaca
     4441 acagcagcgt ctggagcagg cgctgatgac cccggacact gacgaatatt acaacgcggc
     4501 agcactatga tctggcagga gaaaatcgac gcggcgcttg atgcgcggcg tgttgctgat
     4561 gccttgcgtc gccgttatcc ggtggcacaa ggagccggac gctggctggt ggcagatgat
     4621 tgccagtatc tgaacttttc cagtaacgat tatttaggtt taagccatca tccgcaaatt
     4681 atccgcgcct ggcagcaggg cgcggatcaa tttggtgtcg gtagcggcgg ttccggtcac
     4741 gtcagcggtt atagcgtggc gcaccaggtg ctggaagaag aactggccga atggctgggc
     4801 tattcgcggg cgctgctgtt tatttctggt tttgccgcta atcaggctgt cattgccgcg
     4861 atgatggcga aagaggaccg tattgttgcc gaccggctta gccatgcctc attgctggag
     4921 gctgccagtt taagcccgtc gccgcttcga cgttttaccc ataacgatgt cgctcatctg
     4981 gcgcgactgc ttgcttcccc ctgtccgggg cagcaactgg tggtgacaga aggcgtgttc
     5041 agcatggacg gcgatagtgc gccactggcg gaaatccagc aagtaacgca acagcacaat
     5101 ggctggttga tggtcgatga tgcccacggc acgggcgtta tcggggagca ggggcgcggc
     5161 agctgctggc tgcaaaaggt aaaaccagaa ttgctggtgg tgacttttgg caaaggattt
     5221 ggcgtcagcg gggcagcggt gctttgctcc gatacggtgg cggattatct ggtgcaattt
     5281 gcccgccatc ttatttacag caccagtatg ccgcccgctc aggcgcaggc attacgtgcg
     5341 tcgctggcgg tcattcgcag tgatgagggg gatgcacggc gcgaaaaact ggcggcactc
     5401 attacgcgtt ttcgtgccgg agtgcaggat ttgccgttta cgcttgctgg ctcatgcagc
     5461 gccatccagc cattgatcgt cggtgataac agccgtgcgt tacaactggc agaaaaactg
     5521 cgccagcaag gctgctgggt cacggcgatt cgcccgccaa ccgtacccgc tggtactgcg
     5581 cgactgcgct taacgctaac cgcagcgcat gaaatgcagg atatcgaccg tctgctggag
     5641 gtgctgcatg gcaacggtta ataaacaagc cattgctgcg gcatttggac gggcagccgc
     5701 gcactatgag caacacgcag agttacagcg ccagagtgct gacgtcttac tggcaatgct
     5761 tccacagcgt aaatacaccc acgtactgga cgcgggttgt ggacctggct ggatgagccg
     5821 ccactggcgg aaacgtcacg cgcaggtgac ggcgttagat ctctcgccgc caatgcttgt
     5881 tcaggcacgc cagaaggatg ccgcagacca ttatctggcg ggagatatcg aatccctgcc
     5941 gttagcgact gcaaccttcg atcttgcatg gagcaatctc gcggtgcagt ggtgcggtaa
     6001 tttatccacg gcacttcgcg agctgtatcg ggtggtgcgc cccggaggcg tggtcgcgtt
     6061 taccacgctg gtgcagggat cgctccctga attacatcag gcgtggcagg cggtggacga
     6121 gcgcccgcat gctaatcgct ttttaccgcc agatgaaatc gaactgtcgc tgaacggcgt
     6181 gcattatcaa catcatattc agcccatcac gctgtggttt gatgatgcgc tcagtgctat
     6241 gcgttcgctg aaaggcatcg gtgccacgca tcttcatgaa gggcgctacc cacgaatatt
     6301 aacgcgttcg cagttgcagc gattgcaact gtcctggccg cagcaacagg ggcgatatcc
     6361 tctgacgtat catctttttt tgggagtaat tgctcgtgag taaacgttat tttgtcaccg
     6421 gaacggatac cgaagtgggg aaaactgtcg ccagttgtgc acttttacaa gccgcgaagg
     6481 cagcaggtta tcggacggca ggttataaac cggtcgcctc tggcagcgaa aagaccccgg
     6541 aaggcttacg taatagtgac gcgctggcgt tacagcacaa cagcagcctg cagctggatt
     6601 acgcaacggt aaatccttac accttcgcag aacccacttc gccgcacatc atcagcgcac
     6661 aagagggcag gccgatagaa tcattggtaa tgagcgccgg attacgcgcg cttgaacaac
     6721 aggctgactg ggtgttagtg gaaggtgctg gcggctggtt tacgccgctt tctgacactt
     6781 tcacttttgc ggattgggta acacaggaac aactgccggt gatactggta gttggtgtga
     6841 aactcggctg tattaatcac gcgatgttga cggcacaggc aatacgacac gccgggctga
     6901 ctctggcggg ttgggtggcg aatgatgtca cgcctccggg aaaacgtcac gctgaatata
     6961 tgaccacgct cacccgcatg attcccgcgc cgctgctggg agagatcccc tggcttgcag
     7021 aaaatccaga aaatgcggca accggaaagt acataaacct tgccttgttg tagccatcct
     7081 gtatttggtt aaattgcgag cgagatcgcg tcttcgattg actgcaattt aaccaattaa
     7141 attctaaaat aatcacgaaa aaaattttac ttccgcatca tgcgatgtat gtgggaattg
     7201 cccaggcggc gggggatagg ggctgcagac agttatccac tattcctgtg gataaccatg
     7261 tgtattagag ttagaaaaca cgaggcaagc gagagaatac gcggcttgca cgcgaattgg
     7321 cgttaaagac ggctcaaaga aatatctttt attttttaac tggttagata attgcaatgg
     7381 cagtcactga agaggcatct cttgccataa aactgtcatc actcatcttg acaaatgtta
     7441 aaaaagccgt tgctttgggg ataacccggt aaggccggag ttttatctcg tcacagagta
     7501 aattttgctc atgattgaca gcggagttta cgctgtaaca gaaatattat ggtgaggaac
     7561 tgttttttta tccagtataa tttgttggca taattaagta cgacgagtaa aattacatac
     7621 ctgcccgccc aactccttca ggtagcgact catgagtaaa ccgttcaaac tgaattccgc
     7681 ttttaaacct tctggcgatc agccagaggc gattcgacgt ctcgaagagg ggctggaaga
     7741 tggcctggcg caccagacgt tacttggcgt gactggctcc gggaaaacct tcaccattgc
     7801 caatgtcatt gctgaccttc agcgccctac catggtactt gcgcccaaca aaacgctggc
     7861 ggcccagttg tatggcgaaa tgaaagagtt cttcccggaa aacgcggtgg aatatttcgt
     7921 ctcctactac gactactatc agccggaagc ctatgtaccg agttccgaca ccttcattga
     7981 gaaagatgcc tcggttaacg aacatattga acagatgcgt ttgtccgcca ccaaagcgat
     8041 gctggagcgg cgtgatgtgg ttgtggtggc gtctgtttcc gcgatttatg gtctgggcga
     8101 tcctgattta tatctcaaga tgatgctcca tctcacggtc ggcatgatta tcgatcagcg
     8161 cgcgatcctg cgccgactgg cggagctgca atacgctcgt aatgatcagg cattccagcg
     8221 tggtactttc cgcgttcgtg gtgaggtgat tgatatcttc ccagcagaat cggatgacat
     8281 tgcacttcgc gtggagctgt ttgacgagga agtggaacga ttgtcgttat ttgacccgct
     8341 caccgggcag attgtttcca ctattccacg ttttaccatc tacccgaaaa cgcactacgt
     8401 cacgccacgc gagcgcatcg tccaggcgat ggaggagatc aaagaagagc tggctgccag
     8461 acgtaaagtg ctattggaaa acaacaaact gctggaagag cagcggctga cccagcgtac
     8521 ccagtttgat ctggagatga tgaacgagct gggctactgt tccggaattg agaactactc
     8581 gcgcttcctc tccggtcgtg gaccgggtga gccaccgccg acgctgtttg attacctgcc
     8641 tgctgatggg ctgctggtgg tcgatgaatc tcacgttacc attccgcaaa ttggcggcat
     8701 gtatcgcggt gaccgggcgc gtaaagagac gctggtggag tacggtttcc gcctgccatc
     8761 agcgctggat aaccgtccgc tgaaatttga agagttcgaa gcattagcgc cgcaaaccat
     8821 ctatgtttcg gcgacgccgg gtaattacga gctggaaaaa tccggcggcg atgtggtgga
     8881 tcaggtggtg cgtccaaccg gattgcttga tccgattatc gaagtgcggc cagtggcgac
     8941 acaggtcgat gatcttctat cggagattcg tcagcgagcg gcaattaacg aacgcgtact
     9001 ggttacaact ctgaccaagc ggatggcgga agatctcact gaatatctcg aagaacacgg
     9061 tgagcgcgtg cgttatcttc actcagatat cgacaccgtc gaacgtatgg agattatccg
     9121 cgacttgcgt ctgggtgagt tcgacgtatt ggtagggatc aacttactgc gcgaaggtct
     9181 ggatatgccg gaagtttcgc tggtggcgat cctcgacgct gacaaagaag gcttcctgcg
     9241 ttccgaacgt tcgttgatcc agaccattgg tcgtgcggca cgtaacgtta acggtaaagc
     9301 gattctctac ggtgataaga tcaccccatc aatggcgaaa gcgattggcg aaaccgaacg
     9361 tcgccgcgag aaacagcaga agtacaacga ggaacacggc attacgccgc aaggcttgaa
     9421 caagaaagtg gtcgatatcc tggcgctggg gcagaacatt gccaaaacca aagcgaaggg
     9481 cagaggaaaa tcgcgaccga ttgttgagcc ggataatgtg ccgatggata tgtcgcctaa
     9541 agcgttgcag cagaaaatcc atgaactgga agggttgatg atgcaacacg cgcagaatct
     9601 ggagttcgaa gaagcggcac aaattcgtga ccagttgcat cagctgcgtg agttgttcat
     9661 tgcggcttcg tgacaggata acgaagaaga cagatgacaa aagcaaaatt gcctgatgtg
     9721 ctaggcttat caggcctaca tgatctctgc aatacattga attagcgcgg tttgtaggcc
     9781 ggataaggct ttcacgccgc atccggcata aacaacgcgc actttgtctt caataggaag
     9841 ccggaatttt ccttcggctt tccgttaacc taaagcctgt aatgcctttt ccagcgcgct
     9901 atgcaacaac tggcggtcat gacgatacgg aatatcgctg gcctccagta cctcctgaat
     9961 cacaatccgc tctttcaccg ccgagacatc cacttttggc ccgacgatga ccgcatcaat
    10021 gaccttttta ccgacatact gctccataat tgccagcttg ctttccagct tcaaattagc
    10081 cgcaggtaaa ctcaactcac gccccagatt gcctatatag accatcggcg ctggcgtgcg
    10141 gcgtaatgcc tgtgcgattt ccttcagcag cagaattggc atcaggctgg tgtaaaaact
    10201 gccaggccca ataatgatga gatccgcttc actgatagcg tgaacagcct cacgcgttgc
    10261 gggtacattc ggcgttaaca gtaactcctg aatcggcgta gttaactggt cgatattgac
    10321 ctcgccgtaa acttcatgcc cctgatcgtc aatcgccatc agatccaccg gatgctccga
    10381 catcggaatc aaatgtgcgt ccactttcag cagattgcga atcagattga ttgcttcaag
    10441 aggtcgcacg ctaaggtgat ccagtgcctt cagcatcaga tttccaaggt tgtgcccgga
    10501 aagttcgcca ttaccgccga aacgatattc aaacattgcg gaagcgacgc tgggttcagt
    10561 tatcagctgg ttgaggcagt tacgcatatc gccccaggca ataccgcctt ctgagcggcg
    10621 aatacgccct gtcgatccgc cattatccgt ggtggtgacg atacccgtta aacgagaacc
    10681 caaagacgaa agtgatgaga gaacgcgtcc cagtccatgc cctccgccga gagcaacgac
    10741 acgatcaagg tcagccagcg tacgattgcg catatagttt cctggagtca gattatccgc
    10801 gctacagtag cgcaaattgc ggggaaacag caattaacct gccaatattg aggatacaaa
    10861 ctattttctt ctacctctaa aggacgatgt acgctatgcc ttcctgatga tgtatatcaa
    10921 agtaaaaccg ccattttccc ttattctgta gcgaaatagc acgatcatga cgctatatac
    10981 atgattacat agcgaaagtg tggatggtaa aaatctcatt tacacgctag tatcggctta
    11041 accactaaac actctagcct ctgcacctgg gtcaactgat acggtgcttt ggccgtgaca
    11101 atgctcgtaa agattgccac cagggcgaag gaagaaatga cttcgcctcc cgtatctgga
    11161 aaggtgtaca tggcttcaca actgactgat gcatttgcgc gtaagtttta ctacttgcgc
    11221 ctgtcgatta ctgatgtgtg taactttcgt tgcacctact gcctgccgga tggctacaaa
    11281 ccgagcggcg tcaccaataa aggctttctt accgtcgatg aaattcgccg ggttacgcgc
    11341 gcctttgcca gtctgggcac cgaaaaagtg cgcctgacag gcggtgagcc gtctttacgc
    11401 cgcgacttta ccgatatcat cgccgctgtg cgggaaaacg acgctatccg ccagattgcg
    11461 gtcaccacca atggttaccg tctggaacgc gatgtggcga actggcgcga tgcgggactt
    11521 actggcatca acgtcagcgt tgatagcctt gacgctcgcc agtttcatgc cattaccggg
    11581 caggataaat tcaaccaggt catggcaggg atcgatgctg catttgaggc cggttttgag
    11641 aaggtcaaag tcaataccgt gctgatgcgt gatgttaatc atcatcagct cgacaccttt
    11701 ctgaactgga tccagcatcg ccctatccag ctgcgtttca tcgaattgat ggaaacgggt
    11761 gagggcagcg agctcttccg taagcatcac atctctggtc aggttctgcg tgacgagcta
    11821 ctgcgtcgcg gctggatcca ccaattacgt caacgcagcg acggtcccgc gcaagtcttt
    11881 tgtcatccgg attacgccgg agagattggc cttatcatgc cgtatgaaaa agacttctgc
    11941 gccacttgca accgcctgcg cgtttcctcc attggtaaac tccatctctg cctgtttggt
    12001 gaaggcggcg ttaacctgcg cgatctgctg gaagacgata cccagcaaca ggcgctggaa
    12061 gcgcgtattt cagcggcgct gcgggagaag aaacagaccc atttcctgca tcaaaacaac
    12121 accggtatta cgcaaaactt atcgtacatt ggcggctaaa acgtcaaaag gagagatcag
    12181 atgagtcagg taagcactga atttatcccg acccgtattg ctattcttac ggtttctaat
    12241 cgtcgtggtg aagaagacga tacctccggt cactatctgc gcgattcggc gcaggaagcg
    12301 ggccatcacg ttgtcgataa agccattgtg aaagaaaacc gctacgctat tcgcgctcag
    12361 gtatctgcgt ggatcgccag cgacgatgta caagtggtat tgattacggg tggtactggc
    12421 ctgacggaag gtgatcaggc tcccgaagcg ctgctgccgt tgttcgaccg tgaagttgaa
    12481 ggttttggtg aagtgttccg gatgttgtcg tttgaagaga ttggcacctc aacgttgcaa
    12541 tctcgtgcgg tagcgggcgt cgctaacaaa acgctgattt tcgccatgcc gggttcgacc
    12601 aaagcgtgcc gcaccgcgtg ggaaaatatc atcgcgccgc agctcgatgc ccgtacacgt
    12661 ccatgcaatt tccatccaca tttgaagaaa taagtatgtc gcaactgacc catatcaacg
    12721 ccgctggcga agcacacatg gtggatgtct ccgccaaagc ggaaaccgtg cgtgaagcgc
    12781 gggcggaagc ctttgtcacc atgcgcagcg agacgctggc gatgattatt gatggtcgcc
    12841 accacaaagg cgacgtattt gccactgcgc gtatcgccgg tattcaggcg gcaaaacgca
    12901 cctgggatct gatcccgctg tgtcacccgc tgatgctcag caaagttgaa gtcaatttac
    12961 aggccgagcc ggagcacaat cgggtacgta tagaaacctt atgccgcctg accgggaaaa
    13021 ccggtgtcga aatggaagcg ttaaccgcgg cctccgtggc ggcgctgacc atttatgaca
    13081 tgtgcaaagc ggtgcaaaaa gatatggtga ttggtccggt acgtttgctg gcaaagagcg
    13141 gcggcaaatc aggtgacttt aaggtggaag cggatgatta acgttctttt tttcgcccag
    13201 gtgcgcgagt tggtaggaac agatgcaacc gaagtagctg cggatttccc aaccgttgaa
    13261 gcgttacgcc agcacctggc tgcgcagagc gatcgctggg cgctggcgct ggaagatggc
    13321 aaattgctgg ctgccgtcaa caagacgctg gtgagttttg accatccgct gactgacggc
    13381 gacgaagtgg ctttcttccc gccggtaact ggaggttaag atggcagaaa ccaaaattgt
    13441 tgtcggcccg cagccattca gcgtaggaga agagtatccg tggctggcgg agcgtgatga
    13501 agacggtgcg gtagtcacct ttaccggtaa ggtgcgcaac cataaccttg gcgacagcgt
    13561 caaagcatta accctcgaac actatccggg gatgactgaa aaagcactgg cagaaattgt
    13621 cgatgaagcg cgtaaccgct ggccgctggg gcgcgtcacg gtgattcacc gcatcgggga
    13681 attatggccg ggcgatgaaa tcgtttttgt cggtgtcacc agtgcgcatc gcagcagtgc
    13741 gtttgaagcc gggcagttta ttatggatta tctcaaaacc cgcgcaccgt tctggaagcg
    13801 tgaggccacg ccggaaggcg accgctgggt tgaagctcgg gagagcgatc agcaggcggc
    13861 aaaacgctgg tagttttttg ttagccggat caggcaccgc tgcgtccggc aatttcaccg
    13921 ctcagtacat gcgtttacgc tgcgtcgatg cacagcctca tcattttgca gtatccttaa
    13981 gatattcctt atatcttcag gagaaagtca tggacagatt cccacgttct gattcaattg
    14041 tacaaccccg ggctggcttg caaacttata tggcgcaagt ctatggctgg atgaccgttg
    14101 gcttgttgct gaccgcattt gttgcctggt atgcggctaa ttccgcggcc gtgatggagc
    14161 tgttgttcac taaccgtgtc tttttaatcg gtctgatcat cgcgcaatta gcattggtta
    14221 ttgtgttatc agcgatgatt caaaagctga gcgcaggtgt gacgacgatg ctctttatgc
    14281 tttattcggc gctgacgggt cttacgcttt ccagtatttt cattgtttat accgctgctt
    14341 ctatcgccag tactttcgtc gttactgccg gaatgtttgg cgcaatgagc ctgtacggtt
    14401 acaccacgaa gcgcgatcta agcggctttg gcaatatgct gtttatggcg ttaatcggca
    14461 ttgtgctggc atcgctggtc aacttctggc tgaaaagcga agcattgatg tgggcagtta
    14521 cctacattgg cgtgattgtc tttgtcggat tgacggcgta tgacacgcag aaattgaaaa
    14581 atatgggtga gcagattgat acccgcgaca cgtcgaacct gcgcaaatat tccattcttg
    14641 gcgcgttaac cttgtatctg gacttcatca acttgttcct gatgttgttg cggatcttcg
    14701 gcaaccgccg ttaattcttt ttcaccatca atatcccggc catgtgtcgg gatttttttc
    14761 ttacgaaaaa acgacactta acaagtacca ggtataaaat atgccttatg gccgagcgga
    14821 agtgttttcc gtatccataa atacaaaagt attttgtatg cgtggcttta aatttattga
    14881 tggtgaatat taatattttt tctaaggata actaaaagaa tggaatcata ctcgcaaagc
    14941 agcaataaat tagattttca gcacgaagcc aggatattaa acggcatatg gctcattacc
    15001 gctttgggtt tggtgacaac cgcaggactg gccctggtga tcatcaggaa tgccgcatta
    15061 atgagtgaac ggcccatttg ggtaataagt tgcttaatga ttgttttatg gtcaggcatt
    15121 atctcgcatg gacgaaataa gctccttgaa ctggcaggaa aatgccatag cgaagagttg
    15181 tggagtccgg ttagttgcgc ttttacaggt gcattaacac tctattacta ttttatcggc
    15241 ttctttggga tacttgccgc gatagctata acgcttgtct ggcaaaggca tacgcggttt
    15301 tttcattagt cgtaaatgca catactaaag aacttaaccg aacttcacat cgccgcttca
    15361 tttttcgctc gtagcttctt cgcctggctt tccagtagca ggtaacagat cagcgccagc
    15421 agcagtggga taaagtaata cagcacacgg taagcgagta gggcggcgat aatgctacct
    15481 ttggaggtat gctccccagc cagtagtgcg ataaacaccg cttccagcac accaatcccc
    15541 gccggaatat gcacaatcac cccggcaata ctactaacca gcaacacgcc cagtacaaag
    15601 aaatagttca cgctttgacc aagtaacagc cagattatcg ctcccatgac catccagtta
    15661 acgctggaaa tcagcatctg ggcgagggcg aatttccatg aaggcagcac cagcttttgc
    15721 cctttgatgg tcatatggcg gtgcttcgcg aaagcgcaaa accacaaata aaccgcgata
    15781 atcatcaata agccaatgcc gagaatacgc agcgtggttt gatcgacata ccagtgatcc
    15841 ggcagctcca ccacgcctgc ggtaaagata atcccggcca gcaaaatata gcccagccag
    15901 ttggtggtaa tactgagcga gaaaatccgc gtaatagtgc tgcccgataa ccctagccgc
    15961 gagtacaaac gatagcgcat acctatgccg ccgacccagg tactaagcgt caggttgaag
    16021 gcgtagcaga taaacgacac cagcatcacc tggcgcttcg ccagtttgtg accgcagtaa
    16081 aagcgggcga gcagatcata gcagccgtaa atcagataac tgacgaccac cagcccgacc
    16141 gcactgagca acgcaacgcg attgtagtcg cggatgacct tccagacctc ttcccagtcc
    16201 acctttttgg cgtagaccac cagcaacaca atcaccgcga taaaaaacag ccaggtgagg
    16261 atcttctttg ctaagcgcca gcgcgggtgt gatttactca tcagggtttt acccccgtgt
    16321 tttcagtttc tacccgatcc tgcgtttcca ttgtcggctg agcgggcggg tcaacctgcg
    16381 ccagacgtgg tgtgtgtgcc ggaagccagc cgaccagcgc cgggaagtgg cgtaaaaagt
    16441 ggaacgccag cacgctttta gtcaggttcc accaggtgcg tctcggcagc atggtctcat
    16501 cgacctgctg acaatctgcg gcaataatgc cgttcagatt atcgcgcagc gtctggttaa
    16561 aattacgatc gtggatgatg acatttgctt cgagattcag tgacaaactg agcggatcga
    16621 gattactgga ccctactgtc gcccagtgat cgtccatcaa tgccactttg ccgtggagcg
    16681 ggcggcggcg gtactcaaat acctgaacgc cgcctttaac cagatagtta tacagcaagc
    16741 gcgcaccgac tctgacaatc ggcatatccg gttcgccctg aatgatcagt ttgatccgca
    16801 ccccgcgccg tgccgcttta cgcaaggcgt gtaaaaatcg atagccgggg aagaagtagg
    16861 cgttggcgat aatgacttct cgccgcgcct gagtgagcat tttcagatag tgacgttcaa
    16921 tatcatcgcg atgttcttcg ttatcgcgcc agaccagcaa tacctgcgct tctcccggct
    16981 ggcggttctc ttccgctttg tgatgacgtc gccaccagcg tcgtgccgcg ctctgtccag
    17041 gcaggttttc cagctcaaac tggagaatat cttcgacaat cggcccttca aggcgtactg
    17101 cgtaatcctg tttagcctct ggaccgtagc tggacatatg ctcggcggag tagttcagcc
    17161 cgccaataaa ggcaatacgc gcgtcgatca ccacaatttt gcgatgcatc cggcgaaaca
    17221 cattggtgcg cataccaaaa aggcgagggc ggggatcgta gtagcggaac accacgccag
    17281 ctgccgtcag ttcattgaca aactcatcgc tgagatccgg cgaaccgtag ccatccagca
    17341 agacttccgc ttttaccccg cgttgcgctg ctgccaatag cgccgcatgc agctgtttgc
    17401 cgacgtcatc ctcaaaccag ataaacgttt caagaatgat gcgttcttgt gcctctccaa
    17461 tcgccttaaa caccgcggga taatattgct cgccgttttc cagcaactgg atcttattgc
    17521 cttcgcgcca gctacatttc ataaatgaat ctccgcactt aaaggggcat gatcagaaag
    17581 gtgtcgccat gtccgcagcg gcaacgcggt tggcgcgctg gcgctggcat ttttgacgta
    17641 gatcctgtcc agttgtagta gaggaaattg caccggaaac gtgcgcgccg ggcgtccgtg
    17701 ggcgcgggta aaaatctcat ccagcccggc ctgcactttt aacggatgat tagctttttg
    17761 ccgccagtcg ttgaaatcac ccgctaccaa taccggttcg ccgtccggta gctcattcac
    17821 ccattcggcg agcatcgcaa gctgcgcctg acggtgtgcc tcacgcaggc ccagatgtac
    17881 gcacatcaca tgaatcgctt tcccggtcat cggcggcaca atgcggcagt agagcacgcc
    17941 gcgcttttcc gcaccatcga ccgaaacatc acgattctca taatgttcaa tgggataacg
    18001 tgaaagaacg gcgttaccgt gatgtccttc cgggtatacg gcattgcgac cgtaggcaaa
    18061 atcgctccac atagtgtcgg cgagaaactc gtagtgcgag gtatcgggcc agttttccac
    18121 atgcagcgga tgaacttcgt gcgcgcccat cacttcctgc aggcaaacaa tatcggcgct
    18181 gacggtacgc acggcgtcgc gaagttccgg caaaatgaag cgtcggttaa acgcggtaaa
    18241 gcctttgtga atattgatgg tgagcacctt gaacgaaaat tgttgtgttt gatcgggcat
    18301 aattttcctg tctttgcctc ttatctcatt gaaatagtgt agtcggcgtc acaaaaaggt
    18361 gcggtcttac ggaattttcc gtaaagttcg gtactctgaa taagtagaga taaattcttc
    18421 aggagagaag ccatgaagtg gcaacaacgt gttcgtgtcg caactggcct aagttgctgg
    18481 cagattatgt tgcatttact ggtagtggcg ctgctggtgg tgggctggat gagtaaaact
    18541 ctggttcacg tcggcgtagg attatgcgca ctgtattgtg tcacggtagt gatgatgctg
    18601 gtgtttcagc gccaccccga gcaacgctgg cgtgaggtgg cagacgtgct ggaagagctg
    18661 actacgacct ggtattttgg cgcagcgctg attgtgctgt ggctgttgtc ccgcgttctg
    18721 gaaaacaact ttttgctggc aattgcaggg ctggcaatcc ttgccggccc ggcggtagtg
    18781 tctttgctgg cgaaagataa gaagttacat caccttacgt ctaaacatcg cgtacgccgc
    18841 tgaccctgtc gtggccgtta tcaccagtag cggccacaaa ctattccaca caatatccag
    18901 actcgcatcc ttcaaataaa tctgcttggt aatgtccgta aagtggcgaa tagggttaat
    18961 ccacgtcagg ttttgcagcc atactggcat gttttccacc ggagaaacgt aaccggaaag
    19021 aagaatggcg ggcatcataa agacaaacac gccgataaac gcctgctgtt gtgttgaaca
    19081 gagtgatgaa atcaacagac cgaatcccac cagcgataaa ccgtaaatca ccatcgtaaa
    19141 gtagaacagc gccagcgatc cggcgaaggg gatttgatac gcccagatac caatcgccag
    19201 cacaatggtg gcctggaagg tggcgacaat taatgccggt acggccttgc cgatgaagat
    19261 ctgccaggtg gtgagcggcg aaaccagtaa ctgatcgagc gtaccttgtt cacgttcgcg
    19321 ggcaacagaa agtgaagtga cgatcatcac gccaatagtg gtgatcatgg cgatcagcga
    19381 cggcaccaca aaccatttgt agtcgagatt cgggttatac cagttgcgta ccaccagctc
    19441 gctgttgttg ggtttcggtt ttccttccag cagctcctgt tgataatttt tgacgatctg
    19501 ttgcaggtag ttggcggcga tttgcgcact gttggagtta cgcccgtcga ggatcaactg
    19561 caacggcgcg gtctggaagg tatccagttt gcgcgagaag tcagccggga aacgcaccag
    19621 gagtaacgcc ttttgtgtgt cgatggttgg gcggatctcc tgcgggcttt tcagcagcag
    19681 cacatgggta aaggcgctgg cgcgggcaaa acgttgagta agttctaccg agtgttcgcc
    19741 gttatcttca tcgtagatgg cgatggtggc gttagtcact tccagcgtgg cggcgaacgg
    19801 gaacaggctc acctgaatca gcacgggcaa aatcagaatc gcgcgggttt gcggttcgcg
    19861 cagcagcgac tgcaactctt tgcggattaa cgtccataag cgatgaaaca tgctcttctc
    19921 cctaatccag ccgacgtttg gttttcagcc acgtcaggcc gataaacatc accgccgaag
    19981 cgatcaaaaa cagcacgttt accaccagca ccactggaat attcccggcg aggaacaggc
    20041 tttgcagggt gctgacgaaa taacgagcgg gaataatgta cgtcaccgcg cggatcaccg
    20101 cgggcatact gtctatctga aatataaagc cggaaagcat aatcgacggc agaaaagccg
    20161 cgttcagggc gacctgagcg gcattgaact ggttgcgggt aatcgtggaa atcaacagcc
    20221 ccattcccag ggtactgagt aaaaacaggc tggagataaa aaacagaatc agcagcgacc
    20281 cgcggtacgg cacgccgaga ataaacaccg acaccagcat acacagcaac atcgccagca
    20341 tcccgagaaa gtaataaggg atcagcttac acagcagcag ctccgtgcgg gtaatctccg
    20401 tagagagtaa cgcctccatg gtgccgcgtt cccattctcg cgccaccacc agcgaggtga
    20461 gaatcgcgcc gatgaccgtc atgataatgg tcaccgcacc ggggataata aagtgctggc
    20521 taatcgccgc cgggttaaac cagtagcggg tttgtacatc aataagcggt tcaaaagtct
    20581 gcccgttatc ctccgctcgc tgcatttgcc agatctgcca gatcccttcg acatacccct
    20641 gcacaaagtt agcggtattc ggctcactgc cgtcggtgat cacctgaatc ggtgcggtgg
    20701 cgttggcgcg ctccatctgt tcggcgaaat ccacgggaat aaccaccaga ccgcgaattt
    20761 tccccgcctg cattttggcg atcaattcct gacgattatc gctgatggtg gcgtcgatgt
    20821 agggcgaacc ggtcatggtg tgggtgaaat ccagcgccgc ttcgctacgc tgttccagta
    20881 aaatcccgac gcgcagcttg ctggagtcga ggttaatgcc gtagccaaaa ataaacagca
    20941 gcagcagagg gatcaccacc gcaatcagcc agctactcgg atcgcgaacg atctgccgcg
    21001 tctctttgac gcacagtgcc cgcacgcgac gccaggacag gatcggatta ctcattgcca
    21061 tgctccttat cccagtcgtg gatcaactga ataaaggctt gctccatggt gggatcgggt
    21121 tgctcatcgt tagccgactg cgctttcaaa tcgtccggcg tgccgctggc gattaatttc
    21181 ccacggtaca ccaggccgat gcggtcgcaa tattccgctt catccataaa gtgggtggtg
    21241 accatcaccg tgacgccttt ctctaccatg ctattgatgt gcagccagaa ttcacggcgg
    21301 gtgaggggat caacgccgga agtcggttcg tcgagaaaca gaatgtccgg ttcatgcatc
    21361 agcgaacagg ccagcgccag ccgctgttta aaacctaatg gcagttcatc ggtggcgtgg
    21421 gaggcgatac ttttcaggcc gaacgcctcg ctcatacggg agattttttc gttctgcgcc
    21481 cgaccgcgta agccatacac accagagaaa aagcgtaaat tctgttcgac cgtcaggttg
    21541 ccatagagcg aaaatttttg cgccatatag ccgagatgct ggcgcgcttt accggaactc
    21601 tctttcagat ccatgcccag caccagcgcc tgaccagagg tcggcaccag taaaccgcac
    21661 atcatcttaa aggtggtcga tttacccgcg ccgtttggcc ccagcaaacc aaaaatctcc
    21721 ccgcgtttaa cggcaaagtt gacgtgatcg gtggcggcaa aatcaccaaa tttcttggtc
    21781 agttctttcg cttcgatcac cgtctcgtca ggcgtacctt ccaccgtatg caatattgcg
    21841 cccagcggtg attctgaggt tccggcaccg cccagcaaat caataaacgc atcttcaaaa
    21901 cgcggcgtag tttcgttgat attgatttcc ggcattccgt cggcatggcg aatatcgtct
    21961 ggtgtggcct cttttttgag gatcagacgt accgatttcc cctgaatcat gccgtcgctc
    22021 acctgtggca gtttcaaggc gcgttgcaac aatttgcggt tgccctcgtg cgggctggtc
    22081 atcagaaagc tacgtccgga catggtttgt gtcagggccg tcggttctcc ctgatacagc
    22141 aactcgcctt cgttcatcag caacacgtca cggcactgtt cggcttcgtc gagatacgag
    22201 gtgctccaga gaattaacat tccttcgcct gccagctcat gcaccatctg ccacagttcg
    22261 cggcgtgaga tagggtcaac gccgacgccg ggttcatcga gcagcaacac tttcggttcg
    22321 cccaccaggg tacaggccag gccaagtttt tgcttcattc cgccggagag cttgcccgcc
    22381 aggcgtccgg taaacgggcc aagagatgta aattccagca ggcgagcaaa agtctgcttc
    22441 cgcgcttcac cggtgacgct gcgcaaatcc gcgtacagat tgaggttctc catcaccgtc
    22501 agatcttcat acagaccaaa tttctgcggc atatagccga gcacagcgtg cagcgcagcg
    22561 tcatttttga tcggatcaaa gccaatcacc gtggcactgc cgctgtcggg tttcagtaat
    22621 cccgccagca tccgcatcag cgtggttttg cccgcaccgt ccggcccaac cagccccgtc
    22681 acataaccgg cgtgaatggt gcagtcgagc ggtgcgacgg cgggcttgtc catgcccgga
    22741 aagcgttttt ccaggccgtt cagcgtgata acggcatcat tcatgtcctg cctcgtcacc
    22801 gaattgcacc gttaccggca ttccctggcg taacgcatca tcggcgtcgg tcaccacaat
    22861 acgcaggcga tagacgaggt cggtacgcag atccggtgtt tcgacggttt tcggggtaaa
    22921 ttcagcagtc ggcgaaacga aaccaatctg cccgtgatac ggcttgtccg ggcgaccatc
    22981 ggtataaagc aacactttgc gccccggctg ggcctggtca agattacgtt catcaacata
    23041 ggcgcgtacc cacaccggac gcgtcagcga gacggtaaat accgtgccac cttcattaag
    23101 caccgtgccg ggctctaccg cgcgcgttaa cagcgtgcca tcagacggtg ctatcaacgt
    23161 tgagtcctgc aaattcagct ccgcctgcgc cagttgcgcc tgcgcttgtt cgaggctggc
    23221 tttcgcctgg gcgatgtcct gttcgcggtt accggaacgg tactgacgca atttatcctg
    23281 cgctgatttc agcgtggcct gcgcctggtc gcgcgaggag cgggcatttt ccaggtcatt
    23341 tgccgaaatg gtgcggcttt tccacagtcc ttgctggcgg ttatagaagt tctgcgcata
    23401 gtcataggca gcttgcgcct gtttcaccgc tgcggcggcc tgagcgattt cttcatctcg
    23461 atacccggca agcatcaggt catactgcgc ctgagccacc gaaacgcccg ctttcgcctg
    23521 catcagggca atctcatacg gcttgtgatc cagttcgccc agcacctggc ccgctttgat
    23581 cgcatcacct tcgtccaccg ccagcgactc aacgcgcccc ccaacacgga aactaagatt
    23641 caccgtacga atatccacgt tgccatacag cgtcaggccg ttatcctggc ggctttgata
    23701 ccaccagtag cctccggcaa ccacggcggc aagtaccact accgccagtc cgatcacgac
    23761 aggttttttc atcactacaa actcctttgc gataatcctt gcaaaatcag gtcaatatga
    23821 caggtcaccg tctggttgat cagttcggtc ttttcttcat cgaacgcggt ccagccggta
    23881 cgtaacagaa tagtttcttt gccaagacga aacgccagaa tctcaccaat caacgcatgg
    23941 gtatgaagga tcatgcgggt atcgctagcg tcgcagccgg tccaggcggc aatcaggcgt
    24001 gtaaggtgac tgtgtagcgg actaatcacc tgttcgtgca ccaggtggta ggctgccgtg
    24061 ggagagagct gctcacggga gataaatttg ctgaggttga cggtgtcatc ctgggtgagc
    24121 agcttaatca tgtttctgca ggcgcgaagg atcagttcac ggatggctgc ccgatccggc
    24181 tgtggttgtg cgaacaaacg ttcggcctct tcggcgtgtg gacggaactg ctcgccaata
    24241 aaatcagcaa tccactgggc gcaggcgagg tacaaatctt ctttcgaacc gaagtagtag
    24301 gtgatggctg cgatattctg cccggcctgg gcggctatct cgcgggtggt ggcgttcatt
    24361 ccatattcac caaactgtgc cagtgccgca gcaatcagct gttttttcgc ctgttcaccc
    24421 ttgattgtca tggcaggatt attcatcgca cagtccattc ttaatcaaat gattgattaa
    24481 gattatgact ccatagggga gttgtccagt atgaccagga attttagcaa cgccagtcac
    24541 agggataatt tatgcgctgc gtcacaaaaa ctgctacact ccgctccctc atgacattgt
    24601 ggtttttgtc attttccttt tcagtatctc cctgaaaact acaccggtaa cggtcggggc
    24661 ggttcggagt agttatgtct ttcgattctt tgggtttaag ccctgatatc ctgcgcgccg
    24721 ttgccgagca gggttaccgt gaacccaccc ctattcagca gcaggcgatc cctgcggtgc
    24781 tggaaggccg cgacctgatg gccagtgccc agaccggtac cggcaaaaca gcgggcttta
    24841 cgctgccgct gttgcaacat ctgctcactc gccagccgca cgccaaaggg cgtcgtccgg
    24901 tacgtgcgct cattcttacc ccgacccgtg aactggcggc gcagattggc gaaaacgtcc
    24961 gtgattacag caaatatctg aacattcgtt cgctggtggt gtttggcggt gtcagcatta
    25021 acccacaaat gatgaagttg cgcggcggcg ttgatgtgct ggtggcaacg ccgggacgtc
    25081 tgctggacct ggaacatcaa aatgcagtga agctggatca ggttgaaatc ctcgtccttg
    25141 atgaagctga ccgcatgctc gacatgggct ttatccacga tatccgtcgc gtgttaacaa
    25201 aactgccagc gaagcgccag aacctgttgt tctccgcgac cttctctgac gatattaaag
    25261 ccctggcgga aaaactgttg cacaacccgc tggaaatcga agtggcacgc cgcaatactg
    25321 cgtctgatca ggtgacccag cacgttcact ttgtcgataa gaaacgcaaa cgcgaattgc
    25381 tgtcgcatat gattgggaaa gggaactggc agcaggtgct ggtgtttacc cgtaccaaac
    25441 atggcgctaa ccatctggct gaacagctta ataaagacgg catccgtagt gcggcgatcc
    25501 acggtaataa atcgcaaggt gcgcgtactc gtgcgctggc cgattttaaa tcgggcgata
    25561 ttcgtgtact ggtggcaact gacatcgctg cgcgcggtct ggatatcgaa gagttgccgc
    25621 acgtcgtcaa ctatgagtta ccaaacgtac cggaagatta tgttcaccgt atcgggcgta
    25681 ctggccgtgc ggcggcaact ggcgaagcat tgtcgctggt gtgtgttgat gaacacaaac
    25741 tgctgcgtga tatcgaaaaa ctgctgaaaa aagagatccc gcgcattgcg attccggggt
    25801 atgagccgga cccgtcaatc aaagccgaac cgatccagaa cggtcgccag caacgtggcg
    25861 gcggtcgtgg acagggcggt ggtcgcggtc aacagcaatc acgccgtgcg gaaagtggtg
    25921 cgaagtctgg taacgcgaag cccgcagaaa aaccgtctcg tcgtctcggc gatgccaaac
    25981 cggcaggcga acaacaacgt cgccgccgtc cgcgtaaacc tgccgctgcg cagtaatctt
    26041 ttatgccggg ctatgcccgg catgagactg atgacaaaca caaaactgcc tgatgcgcta
    26101 cgcttatcag gcctacgtga actctgcaat atattgaatg tgcatgtttt tgtaggccgg
    26161 ataaggcgct cgcgccgcat ccggcatttc acaacaagca cttgtcagcc atttgcgagc
    26221 gcggcaaaaa gatttctctg ttttaacgcc cacaagccac ccgatgcgtt acttctccgc
    26281 agccaattgt ccgcgcaacg ccattaaaag gtaccccagg cgattcttgc cctgaccatt
    26341 accgctatct ccccagtaag catcattttg cgtatgctca accagttttg cggacgcggt
    26401 tgccagcaag agtgtacgca gttctgcatg ttgttcgaat ttagcgcgaa gcgctttacg
    26461 catcatcctg ctggttattg cgagccgctt tccagaaaca gaaaaaccat tacccctgaa
    26521 aaccgaaaaa tgccacaata ttggctgttt atacagtatt tcaggttttc tcatggcatt
    26581 aaccgccgcg cttaaagcgc aaattgccgc ctggtataag gcgcttcagg aacagatccc
    26641 cgactttatt ccccgtgcgc cgcagcggca gatgattgcg gacgtcgcca aaacgctggc
    26701 cggagaagaa gggcggcatc tggcgattga agcacccacc ggcgttggga aaacgctctc
    26761 ctatttgatc cccggcatcg ccattgcccg cgaagagcaa aaaacgctgg tggtgagtac
    26821 cgccaacgtg gcattgcagg atcagatcta cagtaaagat ttgcctctgc tgaaaaagat
    26881 cattcccgat cttaaattca ctgctgcttt tgggcgtggg cgctacgttt gtccgcgtaa
    26941 tctgacagcg cttgccagta ctgaacccac gcaacaggat ctgctggcgt ttcttgacga
    27001 cgagctgacg ccgaacaatc aggaagagca aaagcgttgt gcaaagttga agggcgatct
    27061 cgacacttat aaatgggatg gtctgcgcga tcatactgat atcgctatag atgacgatct
    27121 ctggcgtcgt ttaagtaccg acaaagccag ctgcctcaac cgcaactgtt actactatcg
    27181 tgaatgcccg ttttttgttg ctcgtcggga gattcaggaa gcggaagtgg tggtggcaaa
    27241 ccatgcgctg gtgatggcgg cgatggaaag cgaagccgta ttgcctgacc cgaaaaattt
    27301 actgctggtg ctggacgaag gccatcacct gccagatgtg gcgcgggatg cgctggagat
    27361 gagtgccgaa atcaccgcgc cctggtatcg actacagctg gacttgttca cgaaactggt
    27421 cgctacctgc atggagcagt ttcgcccgaa gaccatcccg ccgctggcga tccctgaacg
    27481 tttgaatgcg cattgtgaag agttgtatga gcttatcgcc tcgttaaaca acattctcaa
    27541 tctctacatg cctgccgggc aggaggcaga gcaccgtttt gcgatgggcg aactgccaga
    27601 tgaagtgctg gagatctgcc agcggctggc aaaactcacc gagatgctgc gtggtctggc
    27661 ggagttattt cttaacgatt taagtgagaa aaccggcagc cacgatattg tgcgtctgca
    27721 tcggtttatt ttacagatga accgcgcgct ggggatgttc gaggcgcaaa gcaaactctg
    27781 gcggctggcg tctctggcgc aatcttccgg tgcgccggtg accaaatggg cgacgcggga
    27841 agagcgcgaa gggcagctgc atctctggtt tcactgcgtg ggaatacgtg tcagcgatca
    27901 gctggaaagg ctgctgtggc gcagtattcc gcacattatt gtcacttccg caaccttgcg
    27961 ttcgctgaac agtttttcgc gtttgcagga gatgagcggg ctgaaagaga aagcgggcga
    28021 ccgctttgtg gcgctggatt cgccctttaa ccactgcgaa cagggcaaaa ttgttattcc
    28081 ccggatgcgc tttgagcctt ccatcgacaa cgaagagcag cacattgctg aaatggcagc
    28141 ctttttccgt gagcaggtgg agagcaaaaa acatctcggt atgttggtac tgtttgccag
    28201 cggacgggcg atgcagcggt ttctcgacta tgtgacggat ttacgcctga tgttgctggt
    28261 gcagggcgat cagccacgtt atcgcttagt tgaactgcac cgcaaacgcg ttgccaacgg
    28321 tgagcgtagc gtgctggtgg gcttacagtc ctttgccgaa ggtcttgatc tgaaaggcga
    28381 tctgctcagt caggtgcata tccacaagat cgcttttcca cccatcgaca gtccggtggt
    28441 gatcaccgaa ggggaatggc tgaaaagcct caaccgctat ccgtttgagg tgcaaagcct
    28501 gccgagcgct tcgtttaacc tgattcagca ggttgggcga ctgattcgaa gccacggttg
    28561 ctggggggaa gtggttatct acgacaaacg cttgctgacc aaaaattatg gcaagcgact
    28621 actggatgca ttaccggtat ttccgataga gcaaccggaa gtccctgaag gtatagttaa
    28681 aaagaaagaa aaaacgaaat ccccacgccg tcggcggcgt taatgatgtg agtcaggtaa
    28741 ggagtcgtaa atggactatc gcaaaatcat taaagagatc gggcgcggga aaaaccacgc
    28801 gcgcgattta gaccgggata ctgcgcgcgg tctgtatgcc catatgctca atggtgaagt
    28861 ccctgacctt gagttgggcg gcgtattgat tgcgctacgt atcaaagggg aaggggaagc
    28921 agagatgctc ggcttttacg aagccatgca aaatcacacc atcaagctga cgccgcctgc
    28981 aggcaagccg atgccgattg tcatccccag ttacaacggc gcgcgtaaac aggccaacct
    29041 gacgccgttg ttggcgattc tcctgcacaa actcggtttt cccgtggtgg ttcacggggt
    29101 tagcgaagat ccaacccgcg tgctgacaga aaccattttc gaactgatgg gtattacgcc
    29161 aacgttgcac ggcgggcagg cgcaggcgaa gctcgacgaa catcaaccgg tgtttatgcc
    29221 tgtcggcgcg ttttgcccgc cgctggaaaa acaactggcg atgcgctggc ggatgggcgt
    29281 gcgtaacagt gcacataccc tggcgaaact ggcgacgcca tttgccgaag gtgaggcact
    29341 gcgtctttcc agcgtttcgc atccggaata cattggacgc gtcgcgaagt tctttagtga
    29401 tatcggcggg cgggcgctac taatgcatgg tactgaaggt gaagtgtatg ctaatccgca
    29461 gcgctgcccg cagatcaatc tcattgaccg tgaagggatg cgggtactgt atgaaaaaca
    29521 ggacactgct ggtagcgagt tactgccaca agcaaaagat ccggaaacca cggcgcagtg
    29581 gattgagcgt tgccttgctg gcagcgaacc gattcccgaa tcgctgaaaa tccagatggc
    29641 ttgctgcctg atggctacgg gtgaagcggc aactatcagc gacggcctgg cgcgcgttaa
    29701 tcaggcattt taatttttat ctcagccgga tgagccatgt catccggctt ttaccccacc
    29761 gcaatatgaa attcctgcat ctttattgac cttcccgcgc ccggcgtgca gcataaaaaa
    29821 acaacaaaca cataacataa acaggagtta accatggtaa gtggtcatcg ctttgatgct
    29881 cagaggctgc acagttttat tcaggctgta tttcgtcaga tgggtagtga ggaacaagaa
    29941 gcgaaattag tcgccgatca tttaatcgcg gcaaacctgg cagggcatga ttcacatggt
    30001 attggcatga tcccaagcta tgtgcgctcc tggagtcagg ggcatctgca aattaaccat
    30061 catgccaaag tcgttaaaga agcgggggcg gcggtcacgc tcgatggcga tcgcgcattt
    30121 ggtcaggtcg cggcacacga agcgatggcg ctggggattg agaaagcgcg tcagcacggc
    30181 attgccgccg tggcgctaca taactcgcat catatcggcc gtatcggtta ctgggcagaa
    30241 cagtgcgcgg cggcggggtt tgtctctatc cactttgtta gcgtggtcgg tattccaatg
    30301 gtcgcgccgt tccacggtcg cgacagccgc tttggcacca atccgttctg tgtggttttc
    30361 cctcgtaaag ataatttccc gctgttgctc gattacgcca ccagcgccat tgcatttggc
    30421 aaaacccgcg tcgcctggca taaaggcgtc cccgtgccgc caggttgcct gattgacgtt
    30481 aacggcgtgc cgacgacaaa tccggcggta atgcaggagt cgccgttggg ttcgctgttg
    30541 acctttgccg aacataaagg ctacgccctt gcagcgatgt gtgaaattct tggcggggcg
    30601 ctttctggcg gtaaaacgac gcatcaggaa acgttacaaa ccagtcccga tgccattctt
    30661 aactgcatga ccactatcat tatcaacccg gaactgttcg gcgcgccgga ttgtagtgca
    30721 cagaccgaag cctttgccga gtgggtgaaa gcctcgccgc atgatgaaga taaaccgatt
    30781 ttgctaccgg gcgagtggga agtgaacacg cgtcgcgaac ggcaggagca ggggattcca
    30841 ctggatgcgg gaagctggca ggccatttgt gatgcggcgc ggcagattgg tatgccggaa
    30901 gagacgttgc aggctttctg tcagcagtta gccagctaaa aaaaagcccg tccagtggcg
    30961 gacgggcaaa caagggtaac ataggatcaa tgagggttag agcatatgcg tctgtcggca
    31021 aacagacagg gaaatacttg tgctgaacgt agcgtaaacg cctgatccag ccaacggttc
    31081 ggattttgta ggtctgataa gacgcggtag cgtcgcatca ggcatctgtt gccggatgcg
    31141 gcgtaaacgc cttatccgtc ctacgaatta cttataaatt accgcagtac cgcttaattt
    31201 attgttgttg gtggcggaag taatgctgta tccactggca ccggccgccg cggctttctc
    31261 agccagtttc gcttccaggg catcgagggt ggatgcgcca tcggcagaaa ccacgccgat
    31321 tttattcatg ttctgtgcct gggatgccgt taccggttcc gccgcgaaca cgccaaatga
    31381 cagagttgaa agagccatag cagcaacaac agtattgata gttttcataa ttaatctctc
    31441 gcaggtgatt tttgtataag tgacgttgtt tcgtcgatgt gatgagtatc acgttttttt
    31501 acgagagata aaatcgaaga gaattgacca ccttgttcaa ataaattgat tgatatctaa
    31561 cataatgata attcatttat ttgttgaact tatggacttg ttttatgcag cgtggcagtc
    31621 acgacagcgt taaaggcacg gaatgccacg caaaatacaa attatactcg ctatacatcc
    31681 cgctagttgt agttaacgta actggctgtc tttcgaacct ctgcgattat atcctgtata
    31741 agctggtttt tgtaaatctt tctcctgctg acaatgaatg cataagcgca cgccaggaat
    31801 ggcttcccga cgggcctgcg ggataggggc accgcactct tcacattcat acaggctttc
    31861 gccacgcgga atttcacccc gagcgcgggc aatcgcatct tcaattgtac tgttgatctg
    31921 ttcgttgacg gcgtcatcat tagcccaacc ggatgccata tcgtcctccc catatcaatg
    31981 cttgtacagt taagtgtagc taatataggg acgaactcgg gcagttcaag cgtcagatct
    32041 ccgaccattc ccgcagcaga ttatgataaa gattgagcag cgacaggatc tcttcacttt
    32101 cgccgtagtg gcttttcagc gactgaatat tgttgtccag ttcaaacagc atggcgcgct
    32161 ttttatcatc gcggatcatc gactggatcc acataaatga tgccactcgc acgccgcggg
    32221 ttacgggtgt cacgcaatgc aggctgctgg agggatacaa caccagatcg cctgccggga
    32281 gttttacccg atgttgcccg aaggtgtcat taacgaccag ttcgccgccg tcgtagcttt
    32341 ctggatcgct taaaaacagc gtggcagaaa ggtcagtgcg catccagccg ttttgtggat
    32401 ggctgcgtac tgcgccatcc acatgaaaac cataggtttc attgttctga tagcgattaa
    32461 acagcggcgt ggaaagggta cgcggcaagg ccgcggcaaa gaacaaagca tgttggttaa
    32521 ccgcgttcag catctcattt tgcagggcgg cgtacagggc gctgcgggtg tctacttgtt
    32581 gattgttctt aacttgcgca ccttgtgcgc cggtggtgac gcgcccatcc acccattcgg
    32641 cttgttccag ttgttcgcga aaacgggcga cgtcctgtgg cgataacacg ccgggaatgt
    32701 ggtacatcat acaagttctc caaaaagagg ggcttttacc ctacaccgat ttttaacagc
    32761 aggtgctttg ttcatgctgg atgcggcgtg aacgccttat ccggcctacc aaatgatgtg
    32821 aattcaatgc attgcacaac cttgtaggcc tgataagcgc agcgcatcag gcagtcctgt
    32881 tctcgccatc aacataagtg agccctgcgc cccacatctg aatcagaaat gcatattggc
    32941 cgtgagcagg aaggttcttg gctcgcccgg gtgataacgg tagccgctct tgttgattga
    33001 ggcgacgtaa tcggtatcaa acaggttgta gacgtttagc tggaagtcga gattgcgatt
    33061 aacccgatac cccagtttag cgtcggcgac ccaataacct tcggtaaacg ctggcgttcc
    33121 caccgcgccg tcagaacctt tatgcatact gccgatatag cgtgcgcccg caccaacgga
    33181 aatatcatcg gttgcctgat actggctcca taaggtaaag gcgtgctccg gggtatacgg
    33241 cagcgatgag gaaccatcct gggcaacatc tttgccgttt ttgatggttg ctttttgctg
    33301 ggtatagccg ccaatcacct gccacgcggg agtgatattc ccggccacgg atatctcata
    33361 gccttcgacg cgtttcttac cgtattgcga gtaagttccg tcatcatttt gctcaacttc
    33421 attttcgata tcagtacgga acagcgcggc ggtgagcaac aggcgtttat ccagaacctg
    33481 ccatttggtg ccaatctcgc tggtgttggc tttttgcggt ttaaaatcgg tgcggttggc
    33541 actgttaccg ctgccagact gcgcaagggc gaagttgttg ccgcccggag gctgctggga
    33601 aacggcatag ttaatataga cattgccgtt ttccgtcagg tgatacagcg ccccggcttt
    33661 ccagttcacc agattgcccg acttggcggt atcgacggtg gtgaccggag aaccttttgc
    33721 cacgccagca gggcaggtga tggcaccgcg tccgctgccg ccgcaggcgg tggcactgtc
    33781 atattcagta tgataattat ccagacggat cccgccgttc agctcaaaat cacgggtgat
    33841 ttgcagcgta tcgaaggcgt aaattgcgaa ggtatccgtc tgaccattgg cgtttgcgcc
    33901 gttgcgcgtc aggccgccgg gatgaatgct gctgtcagga tgataaatat ttaccgcagg
    33961 taacgtcacc ggattaacgc cgtagttagt ctgcgtttca cgggtaaatt ccacgccggt
    34021 actgacatca tgaccgatag cgccggtata gaacgtcgag gtcaggttgg tctggttggt
    34081 gagaatttta ttactcacat ctttagtatt cgccgtgcgt gaccaggtcc agctattcac
    34141 atcgctggtg ggctgggtaa tattcgacgc cccgcccata atcgccgtca tcaggtaatc
    34201 ctgcttcacg cgcgaccaac gggtagtatt gcgaatggtg gtgttatcgt tgatgtcgtg
    34261 ctcaaaacgc atggtagcgg tgtcggtggt cgaatcgtcg taatcggaat ccgtgccgta
    34321 aaagttatga gtatcaactt ttccggaatg attcagagcc gctgttcccg cagatggggc
    34381 agaataaccc ggtaaaccga tagtcggaat gccgccgtct ggcgtgttgt gctgggtgac
    34441 atgcagataa ttaagataca aacgattcgc tgtaccaagg ccaaaagcga cagaaggggc
    34501 gacgccgtaa cgctcatttt tgactttgtc gcgtccggca tcgtgcgttt tttcgcccat
    34561 tacattcagg cgcaccgcag tggtatcacc aatgacctga ttgacgtcca gcgtgccgcg
    34621 gcggaactag gcgctgccaa tactggcgga ggcgtcaatg ccggaatcat tgcgcggctg
    34681 tttgctgatc atattgatcg agcctgtcgg cgcgctgcgc ccgtagtcgg tgccggacgg
    34741 ccctttaatt acttcgacct gctcggtatt gaaggtgtcg cgcgagacgc tgccgatatc
    34801 gcgaatgcca tcgatataaa tactgttaga ggtatcggca ccgcgcatat aaatggcgtc
    34861 gccagtggtg gagttaccgt tctcacccgc aaaaaacgca cccacgccgg ggacgttttt
    34921 gagcgcgtcg gtaaggttgg ttgcaccctg atctttaatc acttgttcag aaatcaccgt
    34981 catcgtgcgg gtagtatccg ctaccggacg cgagaatttc ggatcggcag attgttgtgg
    35041 cgcataaagc gaaggcgttg atgcttcgac aaccagcgtg tcatccgcgt tagcttgccc
    35101 ttcggcagcg agtgcctgcg ccacaggcgt aataccaata caaagaccgg caaagaacgt
    35161 gagcgaatga aattgtctgg cagggaaatt gcgattgttt tccattttgc aggtgacttt
    35221 ttcttatatg gtgaaatttt cactgtcacg agaaagcatc tctgcggatg cgatgccact
    35281 gttaatcccg tgatagcgtc cgtgcaagtg ataataattt tgataagcat tatcacttgg
    35341 cgcgattttc tatcacccat tgcgcgaaaa agaaatacat ttatttacat tgcgttcata
    35401 tttgatgttt tttgcttaca aattggacga aaactgcatc cagaggcgaa tgcagttacg
    35461 ttggtggggt tatttgtaga tgattgcagt accgaacatc tggtcatttc cgcctgcgga
    35521 attaatgaca taacctttcg cgccttgttc gcgcgctttt tctgccagtt tatcctccag
    35581 atcgcttagg tttgatgcgc cagttgccga aacggtaccg gcgggccgta gttgaccact
    35641 gtccaggtta ctcatcggtt gcgcggcata agccgtgaga gagattccgc tcaggacggt
    35701 ggcaatcagt agtgtgaggc actttttcat aatgacatcc tcataaatac aacgggtagt
    35761 gccagataag tgtaggtcgc ctgcgcgatg aaaatgacgc aaaaaattga cagagatgcg
    35821 cttatttttt gaacaatata tttttacaaa ataagataaa tcaatgacat gcatgatgat
    35881 gatttttatg gtgaattacc ataaaccata gccatgtgaa ggtttttaga aatgataaac
    35941 gcagccagat atttaccctg tcgcgttatg cgggtatctt acgccgctgc ttaaaggaga
    36001 atgctatgtc cgcccagaaa ccggggttgc atccgcgcaa ccgtcatcac agccgctacg
    36061 atctcgccac gctttgtcag gtcaatcctg aactcaggca attcctcacg cttacacccg
    36121 ccggggagca aagcgtagac tttgccaatc cgctggcggt gaaggcgctc aataaggcgt
    36181 tgctggccca tttttacgcc gtagcgaact gggatatccc ggacggtttt ctctgcccgc
    36241 cagtaccggg ccgggcggat tatattcatc accttgccga tttactggct gaagcaagcg
    36301 gaacaattcc agctaatgcc agcattctgg atatcggtgt tggtgcgaac tgtatttatc
    36361 cgctgattgg cgtacatgaa tatggctggc gttttaccgg tagtgaaacc agcagccagg
    36421 cgttaagcag tgcgcaggcg atcatcagtg ctaatccggg gcttaaccgc gccattcgtc
    36481 tgcgtcggca aaaagagagt ggggcgattt ttaacggcat catccataaa aacgagcaat
    36541 acgatgcgac cttgtgtaac ccgccattcc acgattccgc cgctgcggca cgggcaggta
    36601 gtgaacgtaa acgccgtaac ctggggctga acaaagacga tgcactgaac tttggtggtc
    36661 agcaacagga gttgtggtgt gaaggcggtg aagtcgcttt tatcaaaaag atgattgaag
    36721 agagcaaagg cttcgcgaag caggtgatgt ggtttacctc gctggtatct cgtggtgaaa
    36781 acttaccgcc gttgtatcgt gctttgacgg acgtaggtgc ggtgaaggtg gtaaaaaaag
    36841 agatggccca ggggcaaaag cagagccgct ttatagcctg gacctttatg aacgacgagc
    36901 agcgccgccg ttttgtcaat cgccagcgtt aaagcgttgg ctcccccggc ggcaacggtt
    36961 cagccggggc cgcgcccgga gctggcagca cctgataagt ctgcaccggc gcgcgaacac
    37021 ccgccaggtc gaaatgtttt ttcacctggc tgtcgagggc aaagcggacc gtccactgtt
    37081 tgagtggcag cgtggtgaac gaaacacgca gtgtaaacgc ggtattgctt aagccgacaa
    37141 tcccggcaaa gttcggttca ccaataacca gcccgcgaat ttcttcgttt tccatcaatt
    37201 ctgctaccgc atctttcagt gcctgattgg ctttatcagc atcttcatgg cgatcaacat
    37261 cgtagtttgc cactaccgaa ccaataccgc ggacgaagtt agcaaaggtg gttatcgaag
    37321 accacggaat gatgtgatac gccccggtat cctggcgcac gcccacggag cgaatcgaca
    37381 tccgttccac agtgccggtc aacggcccga tagtcaccaa atctccagtg ttcatgccgt
    37441 tttcaaactg aataaatacc ccggtgataa tatctttcac cagcgtttgc gaaccaaaag
    37501 agatagccag acctaatgcc ccggcacctg ccagcaatgg cgcgatatta acgccgattt
    37561 ccgacaacac aatcatgatg gtgatggtac tgataatcac cgccagcgcg ttacggaaca
    37621 gcgtcagcag ggtacgcgta cgggcgctgg gtagtgggcg gccatgaata tccgaagcca
    37681 gccggttttc gatcaaactg gcgagcaccg tccagccaac cgccgagaag aaaagaatga
    37741 gtgcgatacg gatcaggata tctacggttt tctgccccgc accgttttgc agccagttcc
    37801 agaaatcgaa taatccccat gcgctcaaca gcagcattac cgccacgcag actgtcagaa
    37861 tacgcgccgt tttcagcgcc gccgaaagcc agccattcag ccgtttttgc agctccggat
    37921 aatttcgctg agtatgtggc gagagggtga tggttttcgc cagccagcgg gaaaacatac
    37981 cggaaacaaa tgctgcgata ccaataatcg ccaggctgcg caccgttgca cccatcataa
    38041 atttcaggct gttgcccgga tcgaacaacg aaaagaaaaa cagcacgata aaataggcgc
    38101 ttgccagcca gtgccacacc agcgcaaagg cgcggataaa caagctgaaa aaggccagcg
    38161 aatgctccgc aaagttgagc aaatgttggg taatctcttt tttattacga aagatcaggt
    38221 acaacgccca gatggtcatg cacagcataa tgatgacgtt cgccagcgcg ccaatttgta
    38281 cattcacctg attagagata atcggcacgg ccacaatcag gccataacct atcaaactgc
    38341 ttaaccagct caggcgacgg ctccagtaac gggcggtctc gtcatgaatc gtgaacgggc
    38401 gcagctccgc cacatttggg caaaaaatca ggcgtagtac agctttgaaa aattcaatca
    38461 gggcaaaggc gttgagaaac aaactttgtt ggaaagcgat ggtgcgactg cccgcattca
    38521 ggttgtcgct taaaacctga ccgacaaata atgtcagtgc cagtaacagc aggtcgataa
    38581 taaacgcccc gataatcatc gccggaagct gcaaccagtt actgcgctca cgattttttt
    38641 gccgcgccca ctggcccatt ttgcgataca gcggaagtgc gcacaggcga atcagccagt
    38701 aaaaaccaaa cactaatacc gctaacattg aaaaatgggt caacgcattg ctgaaggttt
    38761 gtggattaaa cggcttatgc ggggagccgg tgatattgcg ataaagttgt ccgaagcggg
    38821 cggaaagggc ttcaccataa tggcggctga cttcggtgac tttttgcagc acggtttgct
    38881 cttcaaccag cgtcggcggc acgatcttcg gtacgggttc cgcaggaggc gtagcggcga
    38941 cggtgcgcaa ctggtcgatc aactctttac gtgaggtgtc attatccagc acatccgcca
    39001 gtgcaccata ggccgctttt ttttgttcga tatccggttc cggggccggt tcagtcgttg
    39061 agtccgttgt cgttgtggtt gtaacgccgg gtatggatac cgcgtgggca ggtgcgccca
    39121 gcaggcagaa gaggatgaac aggatccacc gcatgactcc tccagtgaga aaacagagca
    39181 aaaagataag tatagatgct ggaggagggg cgattttaaa tgagaggaat ctggtgtgcc
    39241 tccctttcgg aggaaaggga ggctgagaag ttaagagacg tgctgcaaaa attcctgcaa
    39301 gcgctggctc ggtgggttct tgatcaacac ctgcggatcg ccatcttccg caatccggcc
    39361 tttgtcgata aagatcaggc gcgaagctac tttctcggca aaaccgattt cgtgggtcac
    39421 gattaccatc gtcatccctt cttcagccag atcctgcata accttcagca cttcatggcg
    39481 cagttccggg tcaagagcgg aagtcggttc atcaaacagc atcattttcg gcttcaccgc
    39541 cagcgcgcgg gcaatcgcca cacgctgctg ttgaccacca gaaagttcgg aagggtagtg
    39601 atgtgcacgc tctgccagac cgactttcgc cagcagctca cgcgccagtt tttccgcctc
    39661 ttctttattc gcgccacgca cgcgtagcgg gccaaacatg acgttttcca gcgccgtcag
    39721 atgcgggaag aggtaaaact gctggaacac catacccgct tcctggcgaa tcaggcgctc
    39781 atcaactttc ggatcgttaa ccttcaggcc atcgacaatc agatcgccgg aggtgatttc
    39841 ttccagtttg ttgatgcagc gcagcagggt cgatttaccg gaaccggacg gcccgataat
    39901 caccacgact tcgccctgag caatgttcaa atcgatattg tgcagcacct gggttggacc
    39961 aaaatgcttg gagacgtttt taaattcaat cacaggattt tcatccttct ttccagacga
    40021 cgcagaataa agctcagcac cagggtaata atcagataga acaccgccac ggcgctccag
    40081 atctcaaggg cgcggaagtt accggcaata atttcttgcc cctgacgggt cagttccgcc
    40141 acgccgataa caataaacag cgaggtgtct ttaatgctga tgatccactg gttacccagc
    40201 ggcggcagca tacgacgcag tgccagcggt aaaatgacgt agcgaatggt ttcccaacgt
    40261 gaaagaccga gcgccagtcc tgcttcacga aaacctttgt gaatagacag caccgcacca
    40321 cgcgtgattt ccgcaatgta cgcgccggag ttgatcatga tggtgaccac cgccgcagta
    40381 aacgggtcga tgcgtaagtc gttaaacgcc atcggcaggg cgaaatagat aaacatcacc
    40441 tggacgacga taggtgtgcc gcggatcact tcaataaaga ccagcgcgac gtggttggct
    40501 atccaaccac cgaaggtgcg tgcaaaacct gccagcaatc cgatcaccag gccgccagcc
    40561 agaccgagga ccgagatcca cagggtcatt ttggcacctt caatcagaag cggaatggca
    40621 ggccagatgg cactccagtc aaactgcata tgttgttcct gttaccgtgg tgtaaataat
    40681 caaattcagg gtaattaatg gccccggagc gggtttgcgc ccctcaccct aatcctctcc
    40741 ccagagggga gagggaactg ccagtgcgcc ttacaggcgt agcgttatta tttcggttca
    40801 gtaccgaacc attttttgta gatttcgttg taagttccgt tctcgcgcag ggttttcaac
    40861 gcgccgttga ctttgtcacg cagctcgtcg ctacctttcg ggaacgcaat accgtattgc
    40921 tgtgcttcca gagagtcacc taccgctttg aactgaccgt taccggcggt tttgatgaag
    40981 tacagaatgt ttggcgtatc gtgcagaacg gcgtctgcgc ggttggtgcc cagttccata
    41041 taggcgttat cgatgttcgg gaactgacgc agatctttag ttttgatgtt tgctttcgcg
    41101 taatcaacgg agccagtgcc gctcttcaca gcaaccactt tcccgtcgag atctttcacg
    41161 cttttcacat cgttattgtt ggctttcacc atcactaaca ggccgctttt gtagtagccg
    41221 tcagagaaat cgatcgcttt tttacgctcg tcggtaatgg tgatgcccgc cagcgccaga
    41281 tcgacgtttt tggtttgcag tgccggaatg atcccactga aatccatcgg cttcagttcg
    41341 taatccagct tcagttcttt agcgatggca gcccacagat caacgtcaaa gcctacatat
    41401 ttatcaccct gtttaaattc aaacggaacg aaggcggtat ccgtcgcgac aactaatttt
    41461 ttatccgcgg catgagaaga aaccgcaaaa gccagggtca gtgcagccag tgaaactttt
    41521 aatacagact tcatagcatt tccttttttc tatccacggg acgatcccct gcgtgacatt
    41581 cacatatatg aaaaaatcgt gccaattttg caactcattg atttatcgag atgcggtaaa
    41641 aacgtgatgc acgatttata gggcaatacc ctgaagatgc accattctgg ggcaccaaac
    41701 tggtgcgcta aaattgtgca ctcaacacag tgattattta gcgtaaaacc tgatgaaaaa
    41761 acaatcttta tgtaacgatt gtgtgatgat gtggatacaa aaaatttact taatcagctg
    41821 gggatagcag aaggatgcac taaataagtg cgttgaggca ggctgcaaaa caaaacggcc
    41881 tcctgtcagg aagccgcttt aatcgggtac taaagttctg caccatcagc gatggattta
    41941 ttcgatgtta gactcgataa accacaggaa tttatccagg tcgcgagatg cggcggtcag
    42001 gatatctgcg gtgtcgtcat ctttcgcttc gccaatcgct ttgcgtacgt cattagcgac
    42061 gattgcgtaa cggtcagcca gttctttcag gtgatcctga acgttgtgga tgtccagcgg
    42121 gtaacttttc agcggggttt tgctgttgat aacttgagtg gtccccagag ctacaccgcc
    42181 cagctgcact gcacgttctg ccatggtatc cagatgatcg atcagtgcgg tgcggaagcc
    42241 atccagcatt tcatgtacgg caatgaagtt agcgccgcgc atgttccagt gcgcttgttt
    42301 ggtaatcaaa gaaagatcaa taaactggat aacctggcga ttcagcaact ctactgttgc
    42361 ttttttctcg ctgtcagaga catcgttgcg ggtataaagc agattggtcg cttttgattt
    42421 aactaattta gcggtactca taatttcata tcctcttgat gttatgtccc agtaattaac
    42481 gagactaagt atagcaccag ctatgtgttc cgctattctg gctgttccta tcacactaat
    42541 agtggtaaca agcgtgaaaa aacaaaacca ataaagattc aatgagttag atatattgat
    42601 aagaacaatt cttatttatt tgctcgacag gaattttcgg aataaatatg cttttgcata
    42661 attcgatata taaagacggt gtagaggaa
//
LOCUS       DAPHCV010000028        42384 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.028, whole genome shotgun sequence.
ACCESSION   DAPHCV010000028 DAPHCV010000000
VERSION     DAPHCV010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.028"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            44..1840
                     /locus_tag="RH033_001561"
     CDS             44..1840
                     /locus_tag="RH033_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HDV2990761.1"
                     /translation="MFDKYRKTLVAGTVAITLGLSASGVMAAGFKPAPPAGQLGAVIV
                     DPYGNAPLTALVDLDSHVISDVKVTVHGKGEKGVEISYPVGQESLKTYDGVPIFGLYQ
                     KFANKVTVEWKENGKVMKDDYVVHTSAIVNNYMDNRSISDLQQTKVIKVAPGFEDRLY
                     LVNTHTFTAQGSDLHWHGEKDKNAGILDAGPATGALPFDIAPFTFIVDTEGEYRWWLD
                     QDTFYDGRDRDINKRGYLMGIRETPRGTFTAVQGQHWYEFDMMGQVLEDHKLPRGFAD
                     ATHESIETPNGTVLLRVGKSNYRRDDGVHVTTIRDHILEVDKSGRVVDVWDLTKILDP
                     KRDALLGALDAGAVCVNVDLAHAGQQAKLEPDTPFGDALGVGPGRNWAHVNSIAYDAK
                     DDSIILSSRHQGVVKIGRDKQVKWILAPSKGWEKPLASKLLKPVDANGKPITCNENDL
                     CENSDFDFTYTQHTAWISSKGTLTIFDNGDGRHLEQPALPTMKYSRFVEYKIDEKKGT
                     VQQVWEYGKERGYDFYSPITSIIEYQADRNTMFGFGGSIHLFDVGQPTVGKLNEIDYK
                     TKEVKVEIDVLSDKPNQTHYRALLVRPQQMFK"
     gene            1858..2526
                     /locus_tag="RH033_001562"
     CDS             1858..2526
                     /locus_tag="RH033_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HDV2990762.1"
                     /translation="MSKLGISSLFKSILLTAALAVSFTASAFTEGTDYMVLEKPIPNA
                     DKTLIKVFSYACPFCYKYDKAVTGPVSEKVKDIVAFTPFHLETKGEYGKQASEVFAVL
                     INKDKAAGISLFDANSQFKKAKFAYYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSQA
                     DFEAALKEPAVQETLEKWKASYDVAKIQGVPAYVVNGKYLIYTKSIKSIDAMADLIRE
                     LASK"
     gene            2541..3212
                     /gene="dsbI"
                     /locus_tag="RH033_001563"
     CDS             2541..3212
                     /gene="dsbI"
                     /locus_tag="RH033_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide oxidoreductase DsbI"
                     /protein_id="HDV2990763.1"
                     /translation="MGIKGMWKDLRTSPVDTLVRWQEQRLLWLLMAVAMGALIILAHS
                     FFQIYLYMAPCEQCVYIRYAMFVMVIGGLVAAINPKNIILKLIGCVMAFYGSILGLKF
                     SLKLNDIHHAVHNPDPDSLFGVQGCSTDPTFPFNLPLAQWAPNWFKPTGDCGYDAPIV
                     PDGVTLSSTQQWFVEMYQQSEGWYLLPPWHFMNMAQACMLAFGMCLVLLVIMSGAWAL
                     KIIRG"
     gene            complement(3258..4046)
                     /gene="ygiD"
                     /locus_tag="RH033_001564"
     CDS             complement(3258..4046)
                     /gene="ygiD"
                     /locus_tag="RH033_001564"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HDV2990764.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQTLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMEMPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            4189..4962
                     /gene="zupT"
                     /locus_tag="RH033_001565"
     CDS             4189..4962
                     /gene="zupT"
                     /locus_tag="RH033_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HDV2990765.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(5020..5190)
                     /locus_tag="RH033_001566"
     CDS             complement(5020..5190)
                     /locus_tag="RH033_001566"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2990766.1"
                     /translation="MFIAWYWIVLIALVVVGYLLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(5452..6105)
                     /gene="ribB"
                     /locus_tag="RH033_001567"
     CDS             complement(5452..6105)
                     /gene="ribB"
                     /locus_tag="RH033_001567"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HDV2990767.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(6210..6358)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6480..6779
                     /gene="ubiK"
                     /locus_tag="RH033_001568"
     CDS             6480..6779
                     /gene="ubiK"
                     /locus_tag="RH033_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HDV2990768.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELEARNNSVADLQSPPAIPPIDKAE"
     gene            complement(6814..7014)
                     /gene="glgS"
                     /locus_tag="RH033_001569"
     CDS             complement(6814..7014)
                     /gene="glgS"
                     /locus_tag="RH033_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HDV2990769.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
     gene            7283..7912
                     /locus_tag="RH033_001570"
     CDS             7283..7912
                     /locus_tag="RH033_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HDV2990770.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIITVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            7939..9600
                     /locus_tag="RH033_001571"
     CDS             7939..9600
                     /locus_tag="RH033_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDV2990771.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVESIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQSLPAVIEKSVEPMKSIDGIKIIQVDGLNRGGAAGDAASGSVSGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            9839..9898
                     /locus_tag="RH033_001572"
     CDS             9839..9898
                     /locus_tag="RH033_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDV2990772.1"
                     /translation="MMKLVIILIVLLLVSFQAY"
     gene            complement(10019..11452)
                     /gene="hldE"
                     /locus_tag="RH033_001573"
     CDS             complement(10019..11452)
                     /gene="hldE"
                     /locus_tag="RH033_001573"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HDV2990773.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(11500..14340)
                     /gene="glnE"
                     /locus_tag="RH033_001574"
     CDS             complement(11500..14340)
                     /gene="glnE"
                     /locus_tag="RH033_001574"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HDV2990774.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSIQAKSVLTFSDFLQD
                     SIIAHPEWLTELESQPPQADEWQHYAAWLQEALSDVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAISALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWSQLTGKLTGHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDVLQEDDTTPVLAHLSEDDCKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            complement(14363..15664)
                     /gene="ygiF"
                     /locus_tag="RH033_001575"
     CDS             complement(14363..15664)
                     /gene="ygiF"
                     /locus_tag="RH033_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HDV2990775.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETL
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVEIDGSQIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            15906..16526
                     /locus_tag="RH033_001576"
     CDS             15906..16526
                     /locus_tag="RH033_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDV2990776.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            16590..17828
                     /gene="cca"
                     /locus_tag="RH033_001577"
     CDS             16590..17828
                     /gene="cca"
                     /locus_tag="RH033_001577"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HDV2990777.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLSFRIADETLALM
                     REMTHAGELEHLTPERVWKETENALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVAGWKEQRCPKPE"
     gene            complement(18016..18837)
                     /gene="bacA"
                     /locus_tag="RH033_001578"
     CDS             complement(18016..18837)
                     /gene="bacA"
                     /locus_tag="RH033_001578"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDV2990778.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(18928..19296)
                     /gene="folB"
                     /locus_tag="RH033_001579"
     CDS             complement(18928..19296)
                     /gene="folB"
                     /locus_tag="RH033_001579"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HDV2990779.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            19401..20018
                     /gene="plsY"
                     /locus_tag="RH033_001580"
     CDS             19401..20018
                     /gene="plsY"
                     /locus_tag="RH033_001580"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDV2990780.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(20031..20963)
                     /gene="ttdR"
                     /locus_tag="RH033_001581"
                     /gene_synonym="dan"
     CDS             complement(20031..20963)
                     /gene="ttdR"
                     /locus_tag="RH033_001581"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HDV2990781.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDAIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVRVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            21170..22081
                     /gene="ttdA"
                     /locus_tag="RH033_001582"
     CDS             21170..22081
                     /gene="ttdA"
                     /locus_tag="RH033_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HDV2990782.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            22078..22683
                     /gene="ttdB"
                     /locus_tag="RH033_001583"
     CDS             22078..22683
                     /gene="ttdB"
                     /locus_tag="RH033_001583"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HDV2990783.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            22732..24195
                     /gene="ttdT"
                     /locus_tag="RH033_001584"
     CDS             22732..24195
                     /gene="ttdT"
                     /locus_tag="RH033_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HDV2990784.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTIVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(24238..25251)
                     /gene="tsaD"
                     /locus_tag="RH033_001585"
     CDS             complement(24238..25251)
                     /gene="tsaD"
                     /locus_tag="RH033_001585"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDV2990785.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            25489..25704
                     /gene="rpsU"
                     /locus_tag="RH033_001586"
     CDS             25489..25704
                     /gene="rpsU"
                     /locus_tag="RH033_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDV2990786.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            25815..27560
                     /gene="dnaG"
                     /locus_tag="RH033_001587"
     CDS             25815..27560
                     /gene="dnaG"
                     /locus_tag="RH033_001587"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDV2990787.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLIPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            27755..29596
                     /gene="rpoD"
                     /locus_tag="RH033_001588"
     CDS             27755..29596
                     /gene="rpoD"
                     /locus_tag="RH033_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDV2990788.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(29674..30180)
                     /gene="mug"
                     /locus_tag="RH033_001589"
     CDS             complement(29674..30180)
                     /gene="mug"
                     /locus_tag="RH033_001589"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HDV2990789.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            30305..30380
                     /locus_tag="RH033_001590"
     tRNA            30305..30380
                     /locus_tag="RH033_001590"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30338..30340,aa:Ile,seq:cat)
     gene            complement(30434..31198)
                     /gene="nfeF"
                     /locus_tag="RH033_001591"
     CDS             complement(30434..31198)
                     /gene="nfeF"
                     /locus_tag="RH033_001591"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HDV2990790.1"
                     /translation="MNNTPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            31487..32110
                     /gene="nfeR"
                     /locus_tag="RH033_001592"
                     /gene_synonym="yqjI"
     CDS             31487..32110
                     /gene="nfeR"
                     /locus_tag="RH033_001592"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HDV2990791.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(32214..33734)
                     /gene="aer"
                     /locus_tag="RH033_001593"
     CDS             complement(32214..33734)
                     /gene="aer"
                     /locus_tag="RH033_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HDV2990792.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVACQ
                     ALKVATGERNSVEHLKRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            34152..35531
                     /gene="ygjG"
                     /locus_tag="RH033_001594"
     CDS             34152..35531
                     /gene="ygjG"
                     /locus_tag="RH033_001594"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HDV2990793.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAILAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALSECKKTGDDVAAVILEPIQGEGGVILPRPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCEQVIKAARKALA
                     AMRVSVEEA"
     gene            complement(35573..35905)
                     /locus_tag="RH033_001595"
     CDS             complement(35573..35905)
                     /locus_tag="RH033_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HDV2990794.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            36124..37107
                     /gene="ebgR"
                     /locus_tag="RH033_001596"
     CDS             36124..37107
                     /gene="ebgR"
                     /locus_tag="RH033_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HDV2990795.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYIDQGVNRIGFIGGEDLPGKADIREVAFVEYGRLKHVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLIFVPSKLKLRGTTR"
     gene            37291..40383
                     /gene="ebgA"
                     /locus_tag="RH033_001597"
     CDS             37291..40383
                     /gene="ebgA"
                     /locus_tag="RH033_001597"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HDV2990796.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLIGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGELVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDADGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKVLDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YSDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQAVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            40380..40829
                     /locus_tag="RH033_001598"
     CDS             40380..40829
                     /locus_tag="RH033_001598"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HDV2990797.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            40892..42325
                     /locus_tag="RH033_001599"
     CDS             40892..42325
                     /locus_tag="RH033_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV2990798.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
BASE COUNT        10404 a        11322 c        10631 g        10027 t
ORIGIN      
        1 ttcatcatta ttatatattt gcgttatata aggagatata tccatgtttg ataaatatag
       61 aaaaacactc gtagccggaa ctgtggcgat aaccctgggt ttgtcagcat cgggggtgat
      121 ggctgcgggt tttaaaccag cgccgcctgc cgggcaactg ggtgcggtca ttgtcgatcc
      181 ctacggcaat gcaccactga ccgctttggt tgacttagat agccatgtta tttctgacgt
      241 caaagttacc gtccatggga agggcgaaaa aggtgtagaa atcagctatc ccgtgggtca
      301 ggaatcacta aaaacttacg atggtgtacc gatttttggt ctttatcaga aatttgctaa
      361 caaagtgacc gttgagtgga aagaaaacgg caaggtcatg aaagatgatt atgtggtgca
      421 cacttcggcc atcgtcaata attacatgga taaccgctct atatccgatt tacaacagac
      481 caaagttatt aaagtcgcac cgggttttga agatcgcctc tatctggtta atacccacac
      541 ctttaccgcc caaggttccg atctccactg gcatggtgag aaagataaaa atgccggtat
      601 ccttgatgcg ggtccggcaa ccggcgcact accttttgat atcgcgccct ttactttcat
      661 cgtcgatacc gaaggtgaat accgctggtg gctggatcaa gacactttct acgatggtcg
      721 tgaccgcgat atcaacaagc gtggttatct gatgggtatc cgcgaaacgc cacgcggcac
      781 ctttaccgct gtacaaggtc agcactggta cgagttcgac atgatggggc aggtgctcga
      841 agatcacaaa ctaccgcgcg gatttgctga cgccactcat gagtccattg agacgccaaa
      901 tggcacggta ctgttgcgcg taggtaagag taactatcgt cgcgatgacg gcgtacacgt
      961 caccaccatt cgtgaccata tcctcgaagt cgataaatct ggtcgcgttg tcgatgtatg
     1021 ggatctgacc aagatcctcg atccgaaacg cgatgcactg ctcggcgcgc tggatgcagg
     1081 tgcagtttgc gttaacgttg accttgccca tgcaggacaa caggcaaaac tggaaccaga
     1141 tacaccgttc ggcgacgcgc tgggtgtagg gccaggccgc aactgggcgc acgtgaactc
     1201 tatcgcctat gacgcaaaag atgactctat catcctctcc tctcgccacc agggtgttgt
     1261 gaagattggt cgtgataagc aagtgaaatg gatccttgcg ccctctaaag gttgggaaaa
     1321 accgctggcc agcaagctgc tgaaaccggt tgatgctaac ggtaagccaa ttacctgtaa
     1381 cgaaaatgac ctgtgcgaaa actcagactt cgactttacc tacacccagc ataccgcctg
     1441 gatttccagc aaaggaacgc tcaccatttt tgataatggc gatgggcgtc atctggaaca
     1501 acctgcctta ccaaccatga aatattcccg ctttgtggaa tataagattg atgagaagaa
     1561 aggcaccgtt cagcaagtgt gggaatacgg taaagaacgt ggctacgatt tctatagccc
     1621 aatcacctcc atcattgaat atcaagccga ccgtaacacc atgtttggct tcggtggttc
     1681 tattcatttg ttcgatgtcg ggcagccaac cgtcggtaag ttgaacgaaa tcgattacaa
     1741 aaccaaagaa gtgaaagtgg aaatcgacgt gctgtcagat aaacccaatc agactcacta
     1801 tcgtgcattg ttagtccgtc cacaacagat gttcaaataa ttaagaaggg aataaaaatg
     1861 tcgaaattag ggatctcatc actatttaaa agcatactgc ttaccgcagc tttggctgtt
     1921 tcatttaccg cttctgcatt taccgaaggc acggattaca tggttctgga aaaacctatt
     1981 ccgaatgccg acaaaacgct gattaaggta tttagctacg cctgcccgtt ctgttacaag
     2041 tacgacaaag cggttaccgg tccggtatcg gaaaaagtga aagatatcgt tgctttcact
     2101 ccattccatc tggaaacaaa aggtgaatac ggcaaacaag caagcgaagt ctttgcggtc
     2161 ttgatcaata aagataaagc agcaggcatt tccttatttg atgctaactc tcagtttaag
     2221 aaagccaagt ttgcctacta cgcggcttat cacgacaaaa aagaacgctg gtctgacggt
     2281 aaagatccgg ctgcttttat caaaaccggt ctggatgccg caggtatgag tcaggcggat
     2341 tttgaagcgg cactgaaaga accagcggtt caagaaacac tggaaaaatg gaaagcctct
     2401 tatgatgtcg ccaaaatcca gggggttcca gcctatgtcg ttaatggtaa gtacttgatc
     2461 tacaccaaga gcatcaaatc catcgacgcg atggcagacc ttatccgtga gttagcaagc
     2521 aaataacagg gaaacaggct atgggtatta agggaatgtg gaaagatctt cgcacatcac
     2581 cagttgacac actggtcaga tggcaggagc aacgcttgtt gtggttgtta atggccgttg
     2641 cgatgggcgc gttaattatc ctggcgcact ctttcttcca gatctatctc tacatggcac
     2701 cctgtgagca atgtgtatac attcgctacg ccatgtttgt gatggttatt ggaggattgg
     2761 ttgcagcgat caacccaaag aacatcatcc taaagctaat tggctgcgtg atggcatttt
     2821 acggcagcat tttgggactg aagttttcgc tcaaactaaa cgatatccac catgcagtac
     2881 ataatccgga tcccgattca ctgtttggcg tacagggttg ctctaccgat ccaacttttc
     2941 cctttaacct gccgctggca cagtgggcac caaattggtt caagcctaca ggggattgtg
     3001 gctatgacgc accaatcgtt cctgatggcg tcacactgag cagcacgcaa cagtggtttg
     3061 tggaaatgta tcagcaatct gaaggctggt atctgctacc accatggcat tttatgaata
     3121 tggctcaggc ttgtatgctg gcttttggta tgtgtctggt gctgctggtc attatgagtg
     3181 gcgcatgggc gcttaaaatc atcagaggct aactcagcaa cacccgaagc cagtcgttct
     3241 ggcttcgggt tcttttatta acctatctgc accgacagca tacttaaact gcccatttct
     3301 ataccatcga ccggaatggt aattggctcc tgcccatccc acgcacctaa gacatacaac
     3361 aacggcagat agtgctctgg cgttgggttc gataacgcgc caccttcatg gtcgagataa
     3421 ttcaccagag gatgctgctc taccggccct tgccacgtca gattcgcttt cacatactca
     3481 ttaaacgacg tcgcccacgg atacggtgaa ctatcaccgt gccacttcac tgtgcgcagg
     3541 ttatgtacca cgttaccgct ggcgaccaac attattcctt catcccgcag cgctgccagt
     3601 ttgcgcccca tttcgaaatg ccaggcggca ggtttgctac tgtcgatact caactgcacc
     3661 atcggaatat cggcgtcagg atacatctta atcagcacgc cccacgagcc gtggtcaaag
     3721 ccccaggctt ctttatccag tgtgacaggc actggcgcta acagctcaac caatcgctga
     3781 gccaaagccg gtgagccagg agccggataa tgcgtatcgt acagcgcctg cgggaagcca
     3841 ccaaagtcat gaatcgtggg cggcatctcc atcgcggtca ctcctgttcc acgggtaaac
     3901 caatgagccg aaaccaccac tattgcttgc gggcgtggca atgtcatccc caacgtctgc
     3961 cagctgcggg tatacaaatt atcttccagc acgttcatcg gactaccgtg acctaaaaac
     4021 aatgctggca tacgtgttga agacatgatg atatccttaa ctaaaggtgt cattttgata
     4081 tcctcacaat acgcttgttc ggcggagtaa gaacccggat aacaatgatg atgatcatca
     4141 gttattttga cgatctgcct gaaagtaaag atttataagg agttgtcgat gtcagtacct
     4201 ctcattctga ccatactggc gggggcagcc acgtttattg gcgcgtttct cggcgttctc
     4261 gggcaaaaac cctcgaaccg cttactggcg ttttcgctag gatttgcggc ggggatcatg
     4321 ttgctcatct cattaatgga aatgcttcct gccgcattag ccgccgaagg aatgtcgcct
     4381 gtgttgggtt atggaatgtt tatcttcggt ctgcttggct attttggcct ggaccgcatg
     4441 ttgccacatg cgcatccgca ggatttaatg caaaaatcgg tgcagccgtt gccaaaatcg
     4501 atcaagcgca cagccattct gctcactctc ggcatcagtc tgcataactt cccggaaggg
     4561 attgccacct ttgtcacggc gagcagcaac ctggagctgg gatttggtat cgccctggcc
     4621 gtcgcgttgc acaatatccc tgaaggtctg gcggttgcag gcccggttta tgcagcaacg
     4681 ggttctaaac gcacggcaat tctgtgggcg gggatttctg gattggcaga aattcttggt
     4741 ggtgtgctgg cgtggttaat cctcggtagc atgatttccc cggtggtcat ggcggcaatc
     4801 atggcggcgg ttgcaggaat tatggtggcg ctctcggttg atgaattaat gccgctcgcc
     4861 aaagagattg accctaataa taaccccagc tatggcgtac tatgtggaat gtcagtgatg
     4921 ggattcagtt tagtgctgct acaaacggcg ggaattggtt aaaagcaaca acagaagttg
     4981 tccagcgcat ggacaacttc tgccagcttc actaaagcct tattctacct tttcggctgt
     5041 ctcttctctc gtactgttta agtatttgtt ccgcgcttca agcagtgcgt ctctgtcctg
     5101 gcgaaacgcc cgacaataac gtttcaaatg caggagataa cccaccacaa caagagcaat
     5161 caatacaatc cagtaccagg cgataaacat ccttttaccc ttaatattta acaatactaa
     5221 acatttagcg cataaatttc acatatcctt tttcggatat attcatatgg tcgcataata
     5281 acggaacaat tatatgattt tgatcataat gaaaaagaat gcctgatatt aatgaaattt
     5341 tctcatagaa agagaagcgg aattaacatc ttgcatagca gggcaaaata aaaaccgaag
     5401 ccagctacaa cttcggtttc ttgattaaga cagtaaatta agcagcgatt ttcagctggc
     5461 tttacgctca tgtgcctgac ggtatgccac caagtcttca atagtcacga gcgccatatt
     5521 gtgtttattg gcaaactcaa tgcactctgg tgcacgcgcc atcgtgccat cgtcattagt
     5581 cagctcacac agcacaccag ccggtttaaa gcctgccagc gtcatcagat caatagtcgc
     5641 ttcagtatgg ccgccacgcg tcagtacacc accagcctga gcgcgaagtg ggaaaacgtg
     5701 gcccgggcga ttcagatcag aaggttttgc gccatcggca atcgctgcgc gaacggtcgt
     5761 aatacggtca gcggcagaaa caccggtagt cacaccttca gccgcttcaa tggtcacggt
     5821 aaaaccggtg ccataggcgc tggtgttatt ttctaccatc attggcagat cgagttgttt
     5881 acggcgatct tcagtaatgc acaggcaaac aataccgcta ccgtggcgaa tggtcagcgc
     5941 catctgctca acggtcatgg tttctgccgg gaagatcata tcgccttcgt tttcacggtc
     6001 ttcatcatca agcaccatta caccgcgtcc ttcacgcagc gcagccagtg cattttcaac
     6061 acgttcgaaa ggcgtaccaa aagaggaaag tagcgtctga ttcatggtaa aaaaaacctc
     6121 actaaaatta tggttaccag aatcagggca gtcttaggag tggcggcata tagccaaaat
     6181 aacgtgagcg ggtccatgcc cgacagaatc gttactctct cccatccgga ctctaaccgt
     6241 cggccccgga attacaccgg atctgctgtc ctttgagttc gcacccaaag cgctcgcggg
     6301 ctttcaactg agttgattta ccgccggtgg ggaatttcgc cccgccctga gaataagcgg
     6361 attcactata acgctaatga ttagcggcag caacgcatag cttcacataa ttctggttta
     6421 tgacttaccc ttatcgcact acaatggcac tcaacaccta tcagtacagg gaaactacaa
     6481 tgattgaccc gaaaaaaatt gagcaaatcg ctcgccaggt tcacgaatca atgcctaaag
     6541 gaatcaggga gttcggggaa gatgtggaga aaaaaatccg ccaaacccta caagcgcagc
     6601 tgacgcgtct cgatctggtg agccgcgaag aattcgacgt acagacgcaa gtcctgttac
     6661 ggactcgtga gaaactggca ttactggagc agcgcatcag tgaactggaa gcgcgcaata
     6721 attcggtagc agatcttcaa tctcctccgg ctatcccgcc tattgataaa gcagaataaa
     6781 gcaacacaaa aacgggccag acggcccgta atatcagtgt tctaattcaa gttctctggt
     6841 ttgcatcatc tgttggcaat accaggcata acgtgcgcaa aaccaggttc tatgttcttc
     6901 atccatgtta ccagtaacgg ccagaatatc gaccgggcga ccttctgcgt gcattctggc
     6961 aaaactacgc gccaggaaat cgaaattatt taaagaatta agactatgat ccataagcac
     7021 atcctccggt tatcgcatac cttttaaggc catatgcttt ttctgatttc agtattcccc
     7081 aatcagtgta gtgcctttaa acataacgtg cgtaaatata ttgtccccga tcacactttt
     7141 tagtgaaaaa taaaaaacca tattaaaaga ctttctcttc cagaatttct ctaacgtgtg
     7201 ttattattaa caaagataaa tgtaagcaag gaaactatcc taaagtaaat attgcgtcac
     7261 taaatggaca ttggagtgac atatgatttt attcgccgac tataacaccc cttatctttt
     7321 cgctatttcc tttgtactgt tgatcggcct tttagaaatt tttgcgctta tctgcggtca
     7381 catgctctcc ggtgctcttg atgcacatct tgaccattat gattccataa ccacaggtca
     7441 tattagccag gcacttcatt atctcaatat cggaagattg ccagctctcg tcgttctctg
     7501 cttgctggct ggtttttttg gtcttattgg catcctgttg cagcatgcct gcatcatggt
     7561 ctggcaatca ccactgtcga acctgttcgt ggttcctgtc agtttactgt ttacgattat
     7621 tacggtgcat tataccggta aaatagttgc gccctggatt ccacgcgacc atagttcagc
     7681 tattacagaa gaagaatata ttggtagcat ggcattaatt accggtcatc aagccacttc
     7741 aggtaatccc tgcgaaggaa aactcaccga tcaatttggc caaatacatt atttactact
     7801 ggagcctgaa gaaggaaaaa ttttcactaa aggagacaaa gtgttaatta tttgccgact
     7861 ttctgcaact cgatatttgg cggaaaacaa tccctggccc caaatattgt aatttattaa
     7921 tcaaaaggaa atttaataat ggatgatatt gttaattctg tgccctcctg gatgtttacc
     7981 gcgattattg ccgtatgcat tctgtttatt attggaatta ttttcgccag gctctatcgt
     8041 cgcgcttcgg cagagcaagc ttttgttcgt actggtttag gtgggcaaaa agtggtaatg
     8101 agcggtggcg caatcgtgat gccgatcttt catgaaataa tccccatcaa tatgaatact
     8161 ctgaagctgg aagtcagccg ctcaaccatt gatagcctga ttacgaaaga tcgtatgcgc
     8221 gtcgatgtcg ttgtcgcttt ctttgtgcgg gtaaaaccgt cagtggaaag tattgccacc
     8281 gccgcccaga cgcttgggca acgcaccctg tcgcctgaag acttacgtat gttggttgaa
     8341 gataaatttg tcgatgccct ccgtgcaaca gctgcacaaa tgaccatgca tgagttacag
     8401 gatacccgcg agaacttcgt gcagggcgta caaaataccg tagctgaaga tctgtcgaaa
     8461 aacggcctgg aactggaaag cgtttcactt accaacttta accagacgtc gaaagaacat
     8521 ttcaatccta acaacgcctt tgacgccgaa ggtttaacta agctgacgca ggagacggag
     8581 cgccgtcgtc gcgaacgtaa cgaagttgaa caggatgtag aagttgcggt gcgtgagaaa
     8641 aaccgtgatg cgctttcgcg gaagctggag attgaacagc aagaagcgtt tatgacgctt
     8701 gagcaggagc agcaggttaa aacccgtacc gctgagcaga atgcgaaaat tgcggctttt
     8761 gaggccgagc gtcgtcggga agcagagcag acgcgaattc tggctgaacg acagattcag
     8821 gaaacggaaa tcgaccgcga acaggccgtc cgttccagaa aagttgaagc tgaacgtgaa
     8881 gttcgcatta aagagatcga acagcagcag gtcaccgaaa tcgccaacca gacgaaatcg
     8941 atcgcgattg ccgccaaatc ggaacagcag tcacaagcgg aagcgcgtgc caaccttgca
     9001 cttgcagaag cggtaagcgc ccagcaaaac gtagaaacaa ctcgccagac cgcagaagcc
     9061 gatcgtgcta aacaagttgc cctaatcgct gccgcgcagg atgcagaaac caaagcggtt
     9121 gaactgaccg tgcgggcgaa agcagagaaa gaagccgcag agatgcaggc ggcggcaatc
     9181 gttgagttag ccgaagctac gcgtaaaaag ggtctggcgg aagcagaagc gcaacgtgcg
     9241 ctgaacgacg ctatcaacgt actttctgat gaacaaacca gccttaaatt caaactggcg
     9301 ctattacagt cgttacctgc agtaatagag aaatccgttg agccgatgaa gtctatcgac
     9361 ggcatcaaga ttattcaggt cgatggatta aaccgaggtg gtgctgcggg ggatgcagca
     9421 tcaggcagcg ttagtggcgg aaacctggca gaacaggcat tgtctgccgc actctcttac
     9481 cgcacacagg caccgctgat tgactccttg ctcaatgaaa ttggcgtttc aggcggctca
     9541 ctggctgcat tgacttcacc cttaacctca acaactcccg tcgccgaaaa cgtagaataa
     9601 attttcagct atgaacatcc ctggcacaaa aagccgggga ttttttatat ctgcgttccg
     9661 ctaaaaactg caaatgatca ggtcgttgca acgttttgcg tgaccgctcg gggaaggcaa
     9721 aactgcctca gggaaagcgt tgcgcggggt ccggcgctca tcaacaatcg gggagtcagc
     9781 aaagggctga aacgggaaag cccctcccga ggaaggggcc ttacagaagg aaagggttat
     9841 gatgaagctc gtcatcatac tgattgtgtt gttactagta agtttccagg cttattaaca
     9901 accaattcag aggggggaga aatcctccct tacccttgtt cctttactct aggttgaaaa
     9961 aacaacagcg tcaataggcc tgccaggttc gaagcgagat ctgtgaagcg ctttccggtt
    10021 agcctttttt atcctgttgg atcttcttga taatattagt ggtcgagcaa ccgtcttcaa
    10081 agttgagcac caacacttcg ccgccgttgg cccagacttc tttactcccg gcaatctctt
    10141 ctggtttata atcgccgcct ttcaccagca ggtctggcag gatcccggcg atcaagcgct
    10201 gcggcgtgtc ttcttcaaac gacaccaccc agtcgaccgc ttccagtgcg cccagcacaa
    10261 tcatacgctg ttcgagcggg ttaacggggc gggaatcccc tttcagccgt ttggtggagg
    10321 catcgctgtt gacggcgaca atcaggcggt cacccagctt gcgggcgttt gccagatagg
    10381 agacatgccc ggcgtggaga atgtcaaaga cgccattggt catcaccact ttttcaccgc
    10441 gtttacgcgc tgccgctacg gccagcttca gttcctcttc ggtcatcacg ccaaagccgg
    10501 tatctgcgcg tccacgtaca gcgttttcca actcgatcgg cgaaaccgtg gacgtcccca
    10561 gtttgccgac caccacacca gccgccgcat tggcaaagaa gcaggcttct tccagcgaat
    10621 tacccgctgc caatgttgcc gccagcacac caatcaccgt gtcgcccgca ccggtaacgt
    10681 catacacttc ctgcgcctga gttggcatat gcagcggtgc tttacccggt tgcagcagcg
    10741 acatcccctg ttcggaacgg gtcaccaaca gagccgagag ttcgtaatcg gcaatcagtt
    10801 tcatgccgcg ctcaacaatc tcttcttcag tcttacattt accgacaaca gcttcaaatt
    10861 ccgagagatt cggcgttaac agcgtagcgc cgcggtagcg ctcaaaatcg gtgccttttg
    10921 gatcaatcag caccggaacg cccgctttac gcgccagttg gatcatctgc tgcacgcttg
    10981 ccagcgcacc tttcgcgtag tcagaaagca ccagcgcgcc aatcgaactc agtgcctgat
    11041 taatccgctc gtgcagcggc tgcggatcaa ccccttcgaa accttcttcg aaatccagac
    11101 gaatcagctg ttggttgcgg gaaagtaccc gtaatttggt gatagtcgga tgcgtcggta
    11161 cagaaacaaa gtcgcatttg acgttgacgt cggccagaga tttactcagc gcgcgcgctg
    11221 catcgtcaat gcccgtcaac ccgaccaggc gtgcattagc accgagagaa gcgatattca
    11281 tcgccacgtt agccgcgccg cccggacgtt cttcgatggt attcacttta accacgggca
    11341 ccggcgcttc cggcgagata cggctggtgg gaccgtacca gtaacgatcc agcatcacat
    11401 caccaaccac catcactcct gcacgttcaa actctggcag cgttactttc attcctgtct
    11461 cctgagagat tcaaaatttg cgcgcgataa taccatactt cattcttcca ccagccactt
    11521 ctgccagctt gcccgcacca gttcacgctc tgcggtgaag caatcctccg gcacatggcc
    11581 cggcaattcc tgtaatgcca gatgatgaag ttcatcgcgc agcgtagtgt aagcacgggt
    11641 cagcgccatc gcttcctgct cttccataat gtcgttttgc gccagcagct ccagaatacg
    11701 cacgttgtct gaccagcgcg ttaacttcgg tttttcatga gcgtagcgca acaccagata
    11761 ttgagtaata aattcgatat cggtaattcc gccttcatcg gctttgatat caaagcgatc
    11821 gcgatgttta ttgccgagat gagcgcgcat tttctcgcgc atttcccgca cttccgtttg
    11881 cagagtttta ccttcacgcg gcagcgtcat aatctcgcga cgcaccgcgt caaagtgcgc
    11941 ggtgagctgc ggatcgccgt acactacacg cgcacgcacc agcgcctgat gttcccacgt
    12001 ccaggcctcg tttttctgat aatcggcaaa tgcttctgcg gatgtcacca gcattcccgc
    12061 cgccccggac ggacgcaacc gtgcatccac ttcatacaaa atgccggaag aggtacgcgt
    12121 actgaacaga tgcataatgc gttgcgccag acgcagataa aactgtcgcc cgtcgatttc
    12181 ccgctcgccg tcagtcatcg catccattgg gcaatcatgg aggaagatca ggtcaagatc
    12241 ggaactgtag cctaactccc agccgcccag cttgccgtag ccgaccaccg caaaaccacg
    12301 cccttcgcgt tcgttcaggt gattcggttt accgtagcgg gccaccattt gaacccacgc
    12361 ctgctgaacg acggcatcta tcatagcttc cgccagccag gttaagtgat cgctcacttt
    12421 cattaccggt agcgtaccgg cgatatccgc tgccgcaata cgtaacagtt gcgcctgttt
    12481 gaactgacgc agcgcctcaa gctgttgttc ttcgtcatct tccggcacgc gcagcaaata
    12541 ctggcgcagt tcatcacggt aggcatccgt cgccgtcggc tggtagagag tgtttggatc
    12601 gagcagttca tccagcaata acggatagcg cgccagctgg ctggcaatca tcggcgatgc
    12661 ggcacacaga gaaatcaaat gcttaagcgc cgcaggaaat tcactaagca attctaaata
    12721 ggtggtgcgg gtaacaatcc ccaccagcaa ggcggtaatg cgcgacagcg tgacagcagc
    12781 gtcttcacgc gcacagacat cacttagcag atgcggcatc agatggtcga gcacctgacg
    12841 cccgcgcggc ccgatggtac gcttatccag ctctttgcga aaatcggcaa tcagcgtcag
    12901 cacctgtttg caatcatctt cgctaagatg cgccagcact ggcgtagtgt catcttcctg
    12961 caacacatcc tgccacagct cacgccactg ttccgacagc gactcttcct gagtttcgct
    13021 ttcgtcgtcg ccaatcaatt cgttaaacac ccggcgcaca ttggtcatat gtccggtcag
    13081 cttccctgtt agttgcgacc agtcagcgaa atccattgcc cacgccagcc gcgcacgatt
    13141 aagctcatca gaaggaagcg tctgggtttg ttcgtcgtta atgctttgca gcaggttttc
    13201 cagacgccgc aggaacagat acgccactcg caattgttca gcatcgtttt cagaaagcag
    13261 atgcagcgcg gaaatggcgc tgagcgttgg cagtaaagag cgcgattgca gcgacggttc
    13321 acgcccgccg cgaatgagct gaaacacctg gacgataaat tcaatttcgc gaatgccacc
    13381 tgcgccaagt ttgatgttgt cggtcaaacc gcgtcgacgc acttcacggg caatcattcc
    13441 tttcatgttg cgcagcgact gaatcacgct gaaatcgatg taacgacgga aaacaaacgg
    13501 gcgcagcatc gcacgcaact cgttagcata gacgccttcg ctatcgccca taatccgcgc
    13561 cttgaccatc gcgtaacgct cccagtcgcg cccctgctcc tggtaataat cttccaacgc
    13621 ggcaaagctc agcaccagcg ggccactttc gccaaacgga cgcagccgca tatccacgcg
    13681 atagacgaag ccatcctgcg ttggttgatc cagcacttta atcagccgct gccccatgcg
    13741 ggtaaaaaac tgcgcgttat ccagttcccg gcgtccgccc tgcgtacaac catgttccgg
    13801 ccaggcaaaa atcagatcga tatctgatga gaaattcagc tccccaccgc ccagcttacc
    13861 catgcctaaa atcagcagcg gttgcgcttc gccctgcgca ttgcacggcg ttccccattc
    13921 gcggcagcag gcgtcataca gccagtcacg cgccgcaaca atcagcgtct ccgccagatg
    13981 gctgagctgc tgcaaaatgc tctcttcagt aaccagtgcc agcgtttgcg cccaggcaat
    14041 gcgcaccata atacgccgcc ggaatagccg cagctcgcgc attaacccgg cttcgtcgct
    14101 tacatcactg agcgcctcct gcaaccatgc agcgtaatgc tgccattcgt cggcctgcgg
    14161 cggttggctt tccagttccg tcagccactc tggatgcgca attatgctgt cctgcagaaa
    14221 atcactgaaa gtaagtactg acttcgcctg tatgctaagt gattcctcgg ctaaaggctc
    14281 tggcagccgc tcaacaacgg tctgccagta ctgctgtaac ggtgaagaga gcggcttcat
    14341 aagcgattat atccttgcct gattaacgtt ttccgctgtg caaccagaat ggttcctgat
    14401 tgtttgcctc attacggaaa tgttcaattt caatgcgttg tccggtcgca atagcgtgac
    14461 gcagcccctg ccagttctcc agccaggctt gcgcgacgac aggatcgtag taacccgcca
    14521 gcaacaggat tgagtcaata tcacgcgtca ggcgtggcaa ctggtcacgg tagcgatcgc
    14581 ccaacggctg gcagaaaacg cttttcagtt cagcggcatg gcgggaaaga tggatatcgg
    14641 caaagcgttt gaaggaatcg ctcattttac tctgcgcttt ggcatctaaa aacggctgcc
    14701 aggctttgct taccagccat tcggtcaacg ccagcttcgc cattgccgtt tcggtagagt
    14761 agaccgccgt cacggcagaa accgctgaag cgatggtcgc ttcgcattgg gtcagcaggt
    14821 cacgtaagtg agtgctcgct ttacgtggca cgataccgcc gaacagcatc agtgtatgac
    14881 ggaccaggcc aatggctgcc agcacctgct ctttcgccgc atcgttgccg cgtacccaca
    14941 gttcttcatg atactgccat tgcgctaacg ccagctcgag cgccgcttcc agcccctgtt
    15001 ccacatcagc ttttgccgca acatgtaaaa tggtggtcgg tttgatttca cgcgccggat
    15061 tgccctgcgc cagatgatag ccacgcgctg ctttgctcag gctgccctgg cgtaatccgg
    15121 tttgcgatac cagttggttc gccagtttca gcaccgcgcg cgtgtcgccg ctaagcagtt
    15181 ccagttcaag ctcgcagata ggttcagcaa attcacccgc tttcacttcc ccctggtcga
    15241 gggcgatttc aatttggcta ccatcgatct ctaccagcca cttttcgcga taaaaatcgg
    15301 tgctgaacag cggttgtacg cgggaagcga gatcggcagg caattcgccg ttcggccaga
    15361 cttccgtcgg taactgcgcc aggtcgagcg tcggttcgct caacgccacg ttatattccg
    15421 ggcgctggtg taagccgcct gtcactcttc ctgcaacttt catggtcatc tcatagcgac
    15481 cgttttcgcc acggatacgt aagcccatgt cgtgcccacg cagccagtta tccagcgttt
    15541 cgtagtaaat attcagcaac tgcacggggt catggtgctc gccgcccagc gtattgagat
    15601 ggtcacgcaa cgcctcaacg gcactgtgat taacaataaa ctttaattcg atttcctgag
    15661 ccatggcctt tacttatcgg ttatgtcaca taagtgacga tgaacggcga acttaatgcg
    15721 atcttttttg tcagtagata gtattttgcg ccaaattgcc atgcaacggg caatttgacg
    15781 ggcgtaaaag tttgaagcag tggcaagacc tgacacagag gattccgatt gatgtcgaat
    15841 gctttgcgta gcgacactga tgccactact atcgttccac tttccatgac aataacgaca
    15901 gcctgatgcc aaaattacgc ctgatcggat taactttact cgcacttagc gccactgccg
    15961 tctcacacgc tgaagaaacg cgctatgttt ctgatgaact gaatacctgg gtccgtagcg
    16021 gtccgggaga tcattatcgc ctcgtgggca cggttaacgc cggcgaggaa gtgaccttat
    16081 tacaaactga cgccaacacc aattatgccc aggtgaaaga cagctctggc cgtaccgcct
    16141 ggatcccgtt gaaacaactt agcacagagc caagcctgcg ctcccgtgtg ccagatctgg
    16201 aaaatcaggt caaaaccctg accgataaac tcaccaatat cgataacacc tggaaccaac
    16261 gcaccgcaga aatgcagcaa aaagtggcgc agagcgacag cgtgatcaac gggttaaaag
    16321 aagaaaatca gaaactgaaa aacgagctga ttgtcgcgca gaaaaaggtc gatgccgcca
    16381 gcgtacagct ggatgacaaa cagcgcacca tcatcatgca gtggtttatg tatggcggtg
    16441 gcgtgctggg gcttggcttg ctgcttggtc tggtactgcc gcacctgatc ccaagccgca
    16501 aacgcaaaga tcgctggatg aactaaatcg ccttcttcgt tgcactgtct tattattagg
    16561 aattgtgtat ttgacgaagg gatgatggcg tgaagattta tctggtcggt ggtgctgttc
    16621 gggatgcatt gttagggcta ccggtcaaag acagagattg ggtggtggtc ggcagtacgc
    16681 cacaggagat gctcgacgcg ggctaccagc aggtaggccg cgattttcct gtgtttctgc
    16741 atccgcaaac gcatgaagag tatgcactgg cacgtaccga acggaaatct ggttccggtt
    16801 acaccggttt tacctgctat gccgcaccgg atgtcacgct ggaagatgat cttaagcgtc
    16861 gcgatctgac cattaatgcg ctggcccagg acgataacgg cgagattatc gacccgtaca
    16921 acggtctggg cgatctgcaa aatcgtctgt tgcgccatgt ttcccccgct tttggcgaag
    16981 atccgttacg cgtgttgcgc gtagcgcgtt ttgctgcgcg ttatgcccac ctcagtttcc
    17041 gtattgccga tgaaactctg gcgctgatgc gcgagatgac ccatgcgggt gaactggaac
    17101 acctgacgcc tgaacgggta tggaaagaga cggaaaacgc cctcaccacc cgcaatcccc
    17161 aggtgttctt ccaggtactg cgcgattgcg gcgcactgcg cgttttattc ccggaaattg
    17221 acgccctgtt tggcgttccg gcccctgcca ggtggcatcc ggaaatcgac acgggtattc
    17281 ataccttaat gacgctctca atggcggcga tgctgagtcc gcaggtcgat gtccgtttcg
    17341 caacgttatg ccacgatctc ggtaaaggac tgacgccacc agaactttgg ccgcgtcatc
    17401 atggtcatgg cccggcgggt gtgaagttag tggaacaact atgccagcgt ctgcgcgtgc
    17461 caaatgaaat tcgcgattta gccagactgg tggctgagtt tcacgatctc atccacacct
    17521 tcccaatgct gaacccaaaa accatcgtca aactatttga ttccatcgac gcctggcgta
    17581 aaccgcagcg tgtcgagcaa ctggcgctga ccagcgaggc tgatgtgcgc ggcagaaccg
    17641 gttttgaatc agccgactac ccacaaggcc gctggttgcg cgaagcctgg gaagtggcac
    17701 agtcagtgcc gacaaaagcc gtcgttgaag cgggatttaa aggtgtggag attcgcgagg
    17761 agctgacccg acggcggatt gcggcggtgg ctggctggaa ggaacagcgt tgcccaaaac
    17821 ctgagtgagt gttggatatt ttgccggatg cgacgtaagc gtcttatccg gctacatgtc
    17881 aacgacagtt gtaggcctga taagacgcgc gagcgtcgca tcaggcgttg attgccggat
    17941 gcggcgtgaa cgccttatcc ggcctacgca atctgataag acgcgtcagc atcatatccg
    18001 acactgttct tcacatcaaa agaacacaac gtacaccgca gccgccacaa taaagcgata
    18061 aatggcgaac gggataaacg aaatgcgctt aatcaattgc aggaaggttt taatcgctat
    18121 cagcgccacc acaaaagcgg tgataaaccc aacggcaaac atcgggatat cgccggttgt
    18181 caggaagccc cagcttttgt agagatcgag cgccgttgcg cccatcatca tcggcaccgc
    18241 cagcaggaac gaaaactcgg aagcagcgta acggctcacc cccatcagca tcccacctga
    18301 aatggtcgcc ccggaacggg agaaacccgg ccacagcgcc agacactgga agcaaccaat
    18361 cataaacgcc tgacgatagg tcatatcatc aagacccggc gcacgcggct ctttcggctt
    18421 caggcattca gcggcaatca gcaacagacc gccaacgacc agtgcataca tcacatttat
    18481 cgggttaaac aatgatttaa tcgtgtcgtg gaacaacagc cccagtacca ccgccggaat
    18541 catccccagc aaaatgtgga tcagcgttaa acggcctttg ctttctcctt cgtgctgcaa
    18601 cgggcgacca aagtgaatac caataaggcc aaacagacgt cgccagaaca tgacaaccac
    18661 tgccagaatt gatcctaact ggatcacgac ttcaaaggtt ttcgccgtgt cgccctcaaa
    18721 ccccaacaag tgaccgacaa taatcatatg gcccgtgctg gataccggca gaaattctgt
    18781 caatccttcg accacaccca atattgccgc tatcagcagc gagtgcatat cgctcatcaa
    18841 taaaccccta aattataaaa atgtaacgct tgtccgaact actgcagtat gaccaggtta
    18901 taaccgtttg gtttaacagc tgtaaaatta attattttct ttcagattat tgccacgctc
    18961 aatgattacg ccaacattcg ccgcccgcgc cactgcgcct ggcttgctga gtttgatacg
    19021 cacccacggc gagttgaagc gtgctaacag caactccgcc acctcttcag ccacacgttc
    19081 caccagcgca aaacgcgccc cctcgacgtg gctgactacc gtttctgcaa tgtcagcgta
    19141 actgaggcaa tccgccacat catcactttt cgccgcttta cggttatccc acgccatttc
    19201 gatatcgaac actaacttct gttcgatggt ctgttcccag tcgtaaacac caatagtggt
    19261 gattaccgaa agttgctcta taaatacaat atccatcacg tcctgcctgc tttttggcta
    19321 accggatacc acttccggcg aaatgtgcgt attatccaca gattcatcgt tgaacacgaa
    19381 ttttcaaaac ggaacagctt atgagtgcaa tcgcgcctgg aatgatcctc atcgcgtacc
    19441 tctgcggctc catttccagt gccattctgg tttgccgctt gtgtgggctg cccgatccgc
    19501 gaaccagcgg ctccggcaat ccaggcgcaa ccaatgtgtt acgtatcggt ggcaagggag
    19561 cagccgtagc agtactgatt ttcgacgttc tgaaaggaat gttgcccgtc tggggcgcgt
    19621 atgaattagg tgtcagcccc ttctggctag gcttaattgc catcgccgcc tgtcttggac
    19681 acatctggcc cgttttcttc ggatttaaag gaggaaaagg cgttgctacc gcttttggtg
    19741 ccatcgcacc cattggctgg gatctcaccg gagtaatggc gggaacctgg ttactgaccg
    19801 tgctattgag cggatattcg tcgctggggg cgattgtcag tgcactgatt gctccgtttt
    19861 atgtctggtg gtttaagcca caattcacct tcccggtttc gatgctctct tgcctgatcc
    19921 tgctgcgcca tcatgacaac atccagcgtc tgtggcgtcg tcaggagaca aaaatctgga
    19981 cgaaattcaa aagaaagcgc gaaaaggatc ccgagtgatt tctggtggag ctacataacc
    20041 tgatagcctt cgtcgggctt gcccagccgt tgctggcacc atgccgccag aaattccacg
    20101 cagacacgta atttcatgct gcgatagagc ggctcccggt aaacagccca gatattggcg
    20161 ctttgtgcat actccggcaa tactcgcacc agtttgccac tctccagaaa cggcaacaca
    20221 tcccactcgg aacgcaacat aatccctttg ccctccagcg cccattgcaa cactatctcg
    20281 ccgctattgg aggaaagatg cccgcttacc ttcaccgatt ttttctcctg accgttcccc
    20341 aactcccata ttccgtgggt catatcgcgc tctttggtca ccaggcagtc atgacgactt
    20401 aattcctgta aggattgtgg ctgcggatat ttttgcagat actcaggtgc tgcgcataat
    20461 attcttttat tttttgtcaa aagatgcgca atataataat caggaattgc gtcattaata
    20521 cgaatatcca gatcaatatt atcctgcact aaatcaattt gccgatcgaa caattcaaaa
    20581 tgcacctgta actcaggata attgcgcatc agttcggtaa tggctggagc aatatggctg
    20641 cgcccaaaac caaaactgca accaatgcgg atcatcccct ccgggcgcgt tttgatttgc
    20701 gtgacgtcat cgaccagtcg ctgatattgg gtgaggattt ccagcgcatg ttcgtaacaa
    20761 cgttggccac tttcagtcag cgccacgccg cgagcggagc ggttcagcag agttgttgcc
    20821 agcgtatttt caaggatttg gatccgcttg gtgacaaatg caggcgtttg cccaagcgtc
    20881 gccgctgcag cactaaaact cccgctatgg acgatttcca ccagtacctg taagtctttg
    20941 gctaaaggcc agctatttag cattgttaaa tatccgcagt tttgataaac gcagtgtatt
    21001 tcgcaaaaca ttaccattcc ctgctctggc gcaatattcg ttcttatttt cgcgttgatt
    21061 cacgaactgt taattacata ttcacggtgg caaaaaatat aaaaccacat ttttagtggt
    21121 agtttgtggc ggtgaatttt tccagacaaa tacataaact ggagttgcca tgatgagcga
    21181 aagtaataag caacaggcag tgaataagtt gacggagatt gtcgctaact ttaccgccat
    21241 gatttctacc cgaatgcctg atgacgtggt ggataaacta aaacagctaa aggatgccga
    21301 aacatcgtcg atgggaaaaa tcatctacca cacgatgttc gataacatgc aaaaagcgat
    21361 cgacctgaat cgtcctgcct gtcaggacac cggcgaaatc atgttttttg ttaaggtcgg
    21421 ttcccgtttc ccactgcttg gcgagctgca aagcatactc aaacaagccg tggaagaggc
    21481 aaccgtcaaa gcgccactgc gtcacaatgc ggtagaaatt tttgacgaag tgaacaccgg
    21541 caaaaatacc ggcagcggcg taccgtgggt cacctgggat atcgtccccg acggtgacga
    21601 tgcggaaatc gaagtttaca tggcaggcgg cggctgcaca ctacctggtc gttcgaaagt
    21661 gttaatgccg tcggaaggat acgaaggcgt ggtgaaattc gtcttcgaaa atatctccac
    21721 cctcgccgta aacgcctgtc caccggtact ggtaggcgtg ggcatcgcca cctcggtgga
    21781 aaccgccgct gtgctctcac gtaaagccat tttacgccca attggcagcc gccaccccaa
    21841 tccgaaagcg gcagagctgg agctacgcct ggaagaagga ctcaaccgtc tggggattgg
    21901 tccacaaggg ctgactggca acagttcagt gatgggcgta catatcgaat ctgccgcccg
    21961 ccatccgtca accatcggcg ttgctgtttc tacaggctgc tgggcgcatc gtcgcggcac
    22021 actgctggtt catgccgatc tcacctttga aaatctgtct cacacccgga gcgcgttatg
    22081 aaaaagatcc tgacaacccc gatcaaagct gaagatctgc aagatattcg cgtcggcgat
    22141 gtgatctacc tgaccggtac gctggtgacc tgccgtgacg tttgtcaccg tcgcttgatc
    22201 gaactgaaac gtccgatccc ttacgatctc aacggcaaag cgattttcca cgctggcccc
    22261 atcgtgcgca aaaacggcga caaatgggag atggtatccg tcggcccgac aaccagtatg
    22321 cgtatggaaa gttttgaacg tgaatttatc gaacagaccg gcgtgaaact ggtggtcggc
    22381 aaaggcggaa tggggccgct gaccgaagaa ggctgccaga aattcaaggc gctgcatgtg
    22441 attttcccgg caggctgcgc agtactggcg gcaacccagg tggaagagat tgaagaagtg
    22501 cactggacag aactcgggat gccggagtcg ctgtgggtct gccgggtcaa agagttcggt
    22561 ccgctgattg tttctattga tacccacggc aacaacctga tagccgaaaa caaaaagctg
    22621 ttcgccgaac gccgcgatcc catcgtggaa gagatttgcg agcacgtcca ctacatcaaa
    22681 taaccctccc ggagaggctc accccctctc ctttttcgca ggcataacac gatgaaacct
    22741 tccactgaat ggtggcgata ccttgcgccg ctggcggtca tcgccattat tgctctaatt
    22801 ccggttcccg cagggctgga gagtcatacc tggctctact ttgccgtttt tactggcgtg
    22861 atcgttggac tgatcctcga acccgtgccg ggtgccgtgg tggcgatggt gggtatctcc
    22921 attatcgcca ttctctcgcc gtggctgctg tttagcccgg agcagctcgc ccagccaggt
    22981 tttaagttca ccgccaaatc cctctcctgg gcggtttctg gtttttctaa ttcggttatc
    23041 tggctgattt tcgccgcctt tatgtttggc acaggctatg aaaaaaccgg gcttggacgc
    23101 cgcattgcgc tgattctggt gaaaaagatg ggacatcgca cgctgtttct cggctatgcg
    23161 gtgatgttct ccgagctgat cctggcacct gtaacaccgt ccaactcggc gcgcggtgcg
    23221 gggattatct atcccatcat ccgtaacctg ccaccgctct atcaatcaca accaaacgac
    23281 agcagttcgc gcagcattgg ctcgtacatc atgtggatgg ggattgttgc cgactgcgtg
    23341 accagcgcca ttttcctgac ggcgatggca cctaacttgc tgttaattgg cctgatgaaa
    23401 agcgcatctc acgccacact gagttggggc gactggttcc tcgggatgtt gccgctcagt
    23461 attttactgg ttctgctggt tccctggctg gcttacgtgc tgtacccgcc ggtactgaag
    23521 tctggtgatc aggtgccgcg ctgggcagag acggaactgc aggcaatggg cccgctctgt
    23581 tcgcgtgaaa aacggatgct ggggctgatg gtaggcgcgc tggtgctgtg gattttcggc
    23641 ggtgattata tcgatgccgc gatggtcggt tacagcgtgg tggcgctgat gctgcttctg
    23701 cgcattatca gttgggacga cattgtcagt aataaagccg cgtggaacgt tttcttctgg
    23761 ctggcctcgc ttatcaccct cgctaccgga ctcaacaaca ccggttttat tagctggttt
    23821 ggcaaactgt tagcaggcag cttaagcggt tattcgccaa cgatagtgat ggtggcgttg
    23881 attgtggtgt tttatctact gcgctacttt ttcgccagcg ccacggcgta tacctccgct
    23941 ctcgcgccga tgatgattgc cgccgcgctg gcgatgccgg aaatcccgct gccggtgttc
    24001 tgcctgatgg ttggcgcggc aattggtctg gggagcattc ttacgccata cgccaccgga
    24061 cccagtccga tttactacgg tagtggttat ctgccaacgg tggattactg gcgactgggg
    24121 gcaatttttg ggctgatatt cctcgtgttg ctggtgatta ccggcttact gtggatgccc
    24181 gtggtgttgc tttaagtgag gacggtgccg gaagtgactc tccggcacct aagagtttta
    24241 cgcggccggt aactctgcca gcggccagcg cggacgcacg ctaacgccaa gatccgccgt
    24301 cgcgcctgct ttaaaccgca ccattccggc ataggcgatc atcgcgccgt tatcagtaca
    24361 aaattccgga cgcgcgtaga acacttcgcc acggcgtttt ttcatcattt ccgccagctt
    24421 cgcccgtaac gtgcggttag cactcacgcc acctgccatt accagtcgct taaagcccgt
    24481 ctgatccagc gctcgcttac acttaatcat caacgtatcg accaccgcat cttcaaaggc
    24541 gcgggcgata tcagcacgcg tctggtcgtc ggtgccgtta tcacgaatgg tatttgccgc
    24601 gaaggttttc agaccagaaa agctgaaatc cagccctgga cggtcggtca tcgggcgcgg
    24661 gaagacaaag cgcccggctg taccctgagc cgccattttc gacagtaatg gcccgccagg
    24721 ataatccagc cccagcagct tcgcggtttt atcaaacgct tcaccagccg catcatcgat
    24781 agactcgccg agcagctcgt actgaccaat gccagtcacg ctgattaact gcgtatgacc
    24841 gccggaaacc agcagcgcca caaacggaaa ttccggtggg ttatcttcca gcatcggcgc
    24901 taacagatgc ccttccatat ggtgtacagg gatcgccgga acgttccagg caaacgccag
    24961 agaacgcccc acggtcgcgc caaccagcag tgcgccgact aatccagggc ctgcggtata
    25021 ggccacagca tcaatgtctt ttgccgttaa accagactcc tttagcgccg cctggatcaa
    25081 cggtacggtt ttacgcacat gatcgcggga ggccagctca ggcactacgc cgccgtagtc
    25141 agcgtgcaat ttcacctgac tatacaattg gttggctaac aaaccttttt catcgtcgta
    25201 aatggcgatg ccggtttcat cgcaggaagt ttcaataccc agtacacgca tgacttgttt
    25261 tacctcgctt tattaccgcg cagtgtagga ccaatgcggg ttgatgtaaa actttgttcg
    25321 cccctggaga aagcctcgtg tatactcctc acccttataa aagtcccttt caaaaaaggc
    25381 cgcggtgctt tacaaagcag cagcaattgc agtaaaattc cgcaccattt tgaaataagc
    25441 tggcgttgat gccagcggca aaccgaatta atcaaaggtg agaggcacat gccggtaatt
    25501 aaagtacgtg aaaacgagcc gttcgacgta gctctgcgtc gcttcaagcg ttcctgcgaa
    25561 aaagcaggtg ttctggcgga agttcgtcgt cgtgagttct atgaaaaacc gactaccgaa
    25621 cgtaagcgcg ctaaagcttc tgcagtgaaa cgtcacgcga agaaactggc tcgcgaaaac
    25681 gcacgccgca ctcgtctgta ctaattcccc gagagcgttg ctctccgatc agaccgagtt
    25741 gtagttgtaa ggccgtgctt ccgaaaggaa tgcgcggctt attttcgttt atgaattgct
    25801 aaaaatcggg gcctatggct ggacgaatcc cacgcgtatt cattaatgat ctgctggcac
    25861 gcactgacat cgtcgatctg atcgatgccc gtgtgaagct gaaaaagcag ggcaagaatt
    25921 tccacgcgtg ttgtccattc cacaacgaga aaaccccgtc attcaccgtt aacggtgaga
    25981 aacagtttta ccactgcttt ggatgtggcg cgcacggcaa cgcgatcgac ttcctgatga
    26041 actacgacaa gcttgagttc gtcgaaacgg tcgaagagct ggcggcaatg cacaatcttg
    26101 aagtgccatt tgaagcaggt agcggcccca gccagatcga gcgccatcaa cgacaaacgc
    26161 tttatcagtt gatggacggt ctgaatacgt tttaccaaca atctttacag caacctgttg
    26221 ccacgtctgc gcgccagtat ctggaaaaac gcggattaag ccacgaggtt atcgcccgct
    26281 ttgcgattgg ttttgccccc cctggctggg acaacgtcct gaagcggttt ggcggcaatc
    26341 cagaaaatcg ccagtcattg attgatgcgg gcatgttggt cactaacgat caggggcgca
    26401 gttacgaccg tttccgcgag cgggtgatgt tccccattcg tgataaacgc ggtcgggtga
    26461 ttggttttgg cggacgcgtg ctgggcaacg atacccccaa atacctgaac tcgccggaaa
    26521 cggacatttt ccataaaggc cgccagcttt acggtcttta tgaagcgcag caggataacg
    26581 ctgaacccaa tcgtctgctt gtggtcgaag gctatatgga cgtggtggcg ctggcgcaat
    26641 acggcattaa ttacgccgtt gcgtcgttag gtacatcaac caccgccgat cacatacaac
    26701 tgttgttccg cgcgaccaac aatgtcattt gctgttatga cggcgaccgt gcaggccgcg
    26761 atgccgcctg gcgagcgctg gaaacggcgc tgccttacat gacagacggt cgtcagctac
    26821 gctttatgtt tttgcctgat ggcgaagacc ctgacacgct ggtacgaaaa gaaggtaaag
    26881 aagcgtttga agcgcggatg gagcaggcga tgccactctc cgcatttctg tttaacagtc
    26941 tgatgccgca agttgatctg agtacccctg acgggcgcgc acgtttgagt acgctggcac
    27001 tgccattgat atcgcaagtg ccgggcgaaa cgctgcgaat atatcttcgt caggaattag
    27061 gcaacaaatt aggcatactt gatgacagcc agcttgaacg attaatccca aaagcggcag
    27121 agagcggcgt ttctcgccct gttccgcagc taaaacgcac gaccatgcgt atacttatag
    27181 ggttgctggt gcaaaatcca gaattagcga cgttggtccc gccgcttgag aatctggatg
    27241 aaaataagct ccctggactt ggcttattca gagaactggt caacacttgt ctctctcagc
    27301 caggtctgac caccgggcaa cttttagagc actatcgtgg tacaaataat gctgccaccc
    27361 ttgaaaaact gtcgatgtgg gacgatatag cagataagaa tattgctgag caaaccttca
    27421 ccgactcact caaccatatg tttgattcgc tgcttgaact gcgccaggaa gagttaatcg
    27481 ctcgtgagcg cacgcatggt ttaagcaacg aagaacgcct ggagctctgg acattaaacc
    27541 aggagctggc gaaaaagtga tttaacggct taagtgccga agagcgatcg ggaagccccc
    27601 gacagccgca ctgagaggca gcggcaaata tataagtacg ccctcgtaat tatcgttggc
    27661 ggtaaacaac cgttggattt cagcgttaac ggctgaagga catcgggtca atcgcccaac
    27721 accaacctca tgaaataagt gtggataccg tcttatggag caaaacccgc agtcacagct
    27781 gaaacttctt gtcacccgtg gtaaggagca aggctatctg acctatgccg aggtcaatga
    27841 ccatctgccg gaagatatcg tcgattcaga tcagatcgaa gacatcatcc aaatgatcaa
    27901 cgacatgggc attcaggtga tggaagaagc accggatgcc gatgatctga tgctggctga
    27961 aaacaccgcg gacgaagatg ctgccgaagc cgccgcgcag gtgctttcca gcgtggaatc
    28021 tgaaatcggg cgcacgactg acccggtacg catgtacatg cgtgaaatgg gcaccgttga
    28081 actgttgacc cgcgaaggcg aaattgacat cgctaagcgt attgaagacg ggatcaacca
    28141 ggttcaatgc tccgttgctg aatatccgga agcgatcacc tatctgctgg aacagtacga
    28201 tcgtgttgaa gcagaagaag cgcgtctgtc cgatctgatc accggctttg ttgacccgaa
    28261 cgcagaagaa gatctggcac ctaccgccac tcacgtcggt tctgagcttt cccaggaaga
    28321 tctggacgat gacgaagatg aagacgaaga agatggcgat gacgacagcg ccgatgatga
    28381 caacagcatc gacccggaac tggctcgtga aaaatttgcg gaactgcgcg ctcagtacgt
    28441 tgtaacgcgt gacaccatca aagcgaaagg tcgcagtcac gctgccgctc aggaagagat
    28501 cctgaaactg tctgaagtat tcaaacagtt ccgcctggtg ccgaagcagt ttgactacct
    28561 ggtcaacagc atgcgcgtca tgatggaccg cgttcgtacg caagaacgtc tgatcatgaa
    28621 gctctgcgtt gagcagtgca aaatgccgaa gaaaaacttc attaccctgt ttaccggcaa
    28681 cgaaaccagc gatacctggt tcaacgcggc aattgcaatg aacaagccgt ggtcggaaaa
    28741 actgcacgat gtctctgaag aagtgcatcg cgccctgcag aaactgcagc agattgaaga
    28801 agaaacaggc ctgaccatcg agcaggttaa agatatcaac cgtcgtatgt ccatcggtga
    28861 agcaaaagcc cgccgtgcga agaaagagat ggttgaagcg aacttacgtc tggttatttc
    28921 tatcgccaag aaatacacca accgtggctt gcagttcctt gacctgattc aggaaggtaa
    28981 catcggcctg atgaaagcgg ttgataaatt cgaataccgc cgtggttaca agttctccac
    29041 ctacgcaacc tggtggatcc gtcaggcgat cacccgttct atcgcggatc aggcgcgcac
    29101 catccgtatt ccggtgcata tgattgagac tatcaacaaa ctcaaccgta tttctcgcca
    29161 gatgctgcaa gagatgggcc gtgaaccaac gccggaagaa ctggctgaac gtatgctgat
    29221 gccggaagac aagatccgca aagtgctgaa gatcgccaaa gagccaatct ccatggaaac
    29281 gccgatcggt gatgatgaag attcgcatct gggggatttc atcgaggata ccaccctcga
    29341 gctgccgctg gattctgcga ccaccgaaag cctgcgtgcg gcaacgcacg acgtgctggc
    29401 tggcctgacc gcgcgtgaag cgaaagttct gcgtatgcgt ttcggtatcg atatgaacac
    29461 cgaccacacg ctggaagaag tgggtaaaca gttcgacgtt acccgcgaac gtatccgtca
    29521 gatcgaagcg aaggcgctgc gcaaactgcg tcacccgagc cgttctgaag tgctgcgtag
    29581 cttcctggac gattaatcgg taggccggat caggtgttac gccgcacccg gcactagtcc
    29641 ctccgcacaa acgccacctt ttcggtggcg tttttatcgc ccacgcacca ccagtgcctg
    29701 gtccagctcg cgatacgctt caaccagttt ctccagtgaa acgcgactta aaccgctggg
    29761 atttggcagc acccaaatct gcgtcgaacc aatggtgaga gtttgtttcc cccactgtgc
    29821 accgcgctgg ctgaatccct gttcatatgc ttgtttgccc agaatcgcca acgcctgcgg
    29881 ctgataatct tcaatttttt caatcagctt acgcccgcct gcgtgtagct cctgctttga
    29941 aacttcattg gcttgcaccg ttggacggtc taccagtttg gtgacgccac aacgataatc
    30001 cagcagatgc tgtgcctcct gcggcttcaa ctgacggtcg gtaaacccgg cctgatatat
    30061 caccttccag aagcgatttg ccggatgagc aaagggaaaa ccagtcccgg cggatgaaag
    30121 cccagggttg ataccgcaaa acacgacccg taaccctgga gccaaaatat cctcaaccat
    30181 atttactcct ttctaacatc accaggaaaa gtataacgga ttgaaaatac attgtttata
    30241 aaaacagcag gcgcgcggta atggctggat tgcgacacgg agttacttta taatccgcta
    30301 ccatggcccc ttagctcagt ggttagagca ggcgactcat aatcgcttgg tcgctggttc
    30361 aagtccagca ggggccacca gatatagcaa aggctgacga gaaatcgtca gcctttttct
    30421 ttttatatat cagttacttt gcgtgccagt aagccgctgc acgtacccgc tgtgggtcat
    30481 actgttccgc ttcaaagcgg cggcttaaat tcttaacgac tttgccttcg ccggttatcc
    30541 agatgaagta atcatcggca gggatttgca tctgcgccag acgcgcatct accgcctgct
    30601 catcatgcgc cagccattcg atattaaaac catcaaggtg cgcgagataa tcctgacagg
    30661 cgttatcccg cacgctaacc agcgcactaa cttgcggttt aacggcaagt ttgctcaacg
    30721 tttccaggcg gcggcgcagt gcgggcattc cggattcatc gcagacatac agctgatacg
    30781 cgtaatcctc tggcaccacc agcgaaccgc gcggacctgc caccgtgagt ttatcgcccg
    30841 gttgcgcctg catcgcccag ccgctggcga ccccaccgtc gtgaataaag aaatcgatcg
    30901 ccagttcatg gcgtagttcg tcatacagcg gcgtatagtc acgcgacggt gggcgtggtc
    30961 cttccggcca gacgatgccc tcctccgtta ccgttggcgg cacaaagtga gcgtcaggtt
    31021 gaggaaagaa gagtttgctg tgatcgtcaa agccgcgcga tgtaaaaccg tccagcgcct
    31081 cgccgccaag gacaatgcgc tgaaaaccgg cgctgatacg ctcaacgcgt agcacagtca
    31141 gttcacggaa gcgcagatca ttgcgaacgc gctgtgggta gcggggggtg ttattcattg
    31201 ttatcgcctt catgatggta atcagatata tctaaataaa atttcgcaaa tgataatgat
    31261 tgttaatcat gataaatgca agcgatttgt agaactgata tgtctatagt ctgataagac
    31321 gaaccgcctc ttctcaggca tcattactca acgccggatg cggcgtgaac gccttatccg
    31381 gcctacgtgt gagatgagtc cacgtttaaa aatcattatt gtacttgcag taatgctcat
    31441 atcagatata aattagatat atctaattaa acaaaaggag gctgatatga gccatcatca
    31501 cgaagggtgt tgtaaacatg aaggccagcc acgccatgag ggctgctgca aaggtgagaa
    31561 gtcagaacac gagcactgcg gacacggtca ccagcatgaa cacggtcaat gctgcggtgg
    31621 tcgccacggt cgcggcggcg gtcgtcgaca acgtttcttt ggtcacggtg aattgcgtct
    31681 ggtgattctg gatattctct cgcgcgatga cagccacggt tacgagttga ttaaagcgat
    31741 tgaaaatctg acccagggga attacacccc aagcccgggc gtcatctacc caacgctgga
    31801 ttttctgcag gagcagtcgc tgattaccat tcgcgaagag gaaggtggta agaagcagat
    31861 tgcgctgacc gaacaaggcg cgcagtggct ggaagaaaac cgcgaacagg tagagatgat
    31921 tgaagaacgc atcaaagcgc gttgcgttgg cgctgcgctg cgccagaacc cgcaaatgaa
    31981 gcgggcgctg gataatttta aagcggtact ggatttacgc gtcaaccaga gcgatatcac
    32041 tgatgcacaa ataaaaaaga tcattgcggt gatcgaccgc gccgcttttg atattacgca
    32101 actggattaa tcaccgattg ccggatgtgg cgtgccctac ccccgtaggc ctgataagat
    32161 gcgccagcat cgcatcaggc attgtgcgcc aaccgccgga tgcggcatat cgattaatgc
    32221 agcaccgtca ccgcgtcttc cagtcggctg gcgcggtgtt tcaccatcgc cgacacctgc
    32281 gcactctctt ccactaactc ggcgtttttc tgggtgatca ggttaagctc atccactgca
    32341 cgggtcaggc tggaaagccc atcggcctgt tccagcgttg aatggctaat ctgagctatc
    32401 aactgggtga cgttttttac ctgtgccaca atatcttcca tcgtccgtcc ggcggcgtgt
    32461 acctgctgcg aaccggattg caccttatca gcactggcat caatcagctt gcgaatatcg
    32521 ttggcagcat tggcgctgcg gctggctaaa tgacgcactt cccccgccac cactgcaaaa
    32581 cctttgccct gttcgccggc acgcgccgct tccaccgccg cattcagggc cagaatattg
    32641 gtctgaaacg caatatcgtt aatcagcgaa gtaatggtgc caatgcgctg ggtactgtcg
    32701 gcaatatcgt ccattgtctt gatcaccgta gtcatcgcct ccccgccctg cactgccgca
    32761 ttactggcgg tgatagaaag tttatcagca gccgacgccg tggcagagtt ctgtttcacc
    32821 gacgccgcca tctggttcat ggtcgccacc gtttgctgaa cgttatcaac tgtctgctgg
    32881 gtatgttcgt tcagttcatc ggtgcctttc gccagcgtct cactgccatt tctgacgctg
    32941 gaaacctggc ttgagacatc gttaattagc caacggcaca tcaggccaag ctgccctacc
    33001 gcgcgtaatg tcagccccag ctcatcgctg cgcttcagat gctcaacact attacgctct
    33061 ccggtcgcca ccttcagtgc ctgacaggcg acattttcta tcgggcggac aatctgccat
    33121 tcaaaacaag cgcttgccaa caataccact aacgcacaga ggatatacgt caccaccggg
    33181 gcagcaacaa accaaagcat ggccgccagc aagataaaca tcagggtcat cactccacgc
    33241 gcccgccagc gaagcggtaa tgaaggcagt ttacccagcc agcctttacg caccaccagg
    33301 cctttatgaa tacgcttact ggtacgtccg gcgttcaacg ctttgtacag cggctccacc
    33361 gccgcgatct cttcatccgt cgcccgggta cgaatcgaca tatagccact gatttttccc
    33421 tcgcgcacca tcggtaccgc attggcccgc acccaataat ggtcgccatt tttgcggcga
    33481 tttttcacga tgccgctcca gggctcccct tttttcaggg tgaaccacat atctgcaaac
    33541 gccgcttttg gcatatccgg gtgacgcacc atgttgtgcg gctgcccctg taactcttgc
    33601 aaggtaaagc cgctcacctg cacaaaagtg tcattagcat gagtgatata gctttgcaga
    33661 tcggtagtgg acatcagagt ggtgtcgtcc gccagcgggg tattttgctg ggtgacatac
    33721 ggatgagaag acatgttcgc gtcctgtgca ggttatatgg ttgttaactt cttgtcagag
    33781 tttatgtcgg ccccgctgcg gttatcttta actgattaat ttgatttaga tcgcaatttg
    33841 cgattaaagc ataaatctaa tcccttgatt taaaatactt tcactctgtt actatacgaa
    33901 aacgttaatt atcttgccca aaaatcaggc agttgttgcc ctgaaagtgt gcatttgcgc
    33961 agcaatcgcc aaatccatac ccgacaaaaa ccgtgcaaaa taacaacaga tgttaacaaa
    34021 tagcattaaa tattgcacaa atgataaccg catttgtgtt tatcccgatt ttcgcgatcg
    34081 cagccggtgt ggcgcaatcc ctgcaatact taaatcggta tcatgtgata cgcgagcctc
    34141 cggagcatat tttgaacagg ttaccttcga gcgcatcggc tttagcgtgc agcgcccacg
    34201 ccctgaatct cattgaaaag cgaacactag atcatgagga gatgaaagca cttaaccggg
    34261 aggtgattga atacttcaaa gagcatgtca atccggggtt tttagagtat cgcaaatctg
    34321 ttaccgccgg cggggattac ggagccgtag agtggcaagc gggaggttta aatacgcttg
    34381 tcgacaccca gggacaggag tttatcgact gccttggagg ttttggaatt ttcaacgtgg
    34441 ggcaccgtaa tccagttgtg gtttccgccg tacagaatca acttgcgaaa caaccgctgc
    34501 acagccagga gctgctcgat ccgttacggg cgattctggc gaaaaccctt gcggcgctaa
    34561 cgcccggtaa actgaaatac agcttcttct gtaatagcgg caccgagtcc gttgaagcag
    34621 cgctgaagct ggcgaaggct tatcagtcac cgcgcggcaa gtttactttt attgccacca
    34681 gcggcgcgtt ccacggtaaa tcacttggcg cgctgtcggc cacggcgaaa tcgaccttcc
    34741 gcaaaccatt tatgccgctg ctgccagggt tccgtcatgt accgtttggc aatatcgaag
    34801 ccatgcgcac ggcccttagc gagtgcaaaa aaaccggtga tgatgtggct gcagtgatcc
    34861 tcgaaccgat tcagggcgaa ggtggcgtca ttctgccgcg gccgggctat ctcaccgccg
    34921 tgcgtaagct gtgcgatgag ttcggcgcac tgatgatcct cgatgaagtg caaaccggta
    34981 tggggcgcac gggcaagatg ttcgcctgcg agcatgagaa cgtacagccg gacatcctgt
    35041 gtctggcaaa agccctcggc ggcggcgtga tgccgattgg cgcaaccatc gccactgaag
    35101 aggtgttctc cgtgctgttc gacaacccgt tcctgcatac caccaccttt ggcggcaacc
    35161 cgctggcctg tgcggcggcg ctggcgacca tcaatgtgtt gctggagcag aacttaccgg
    35221 ctcaggctga acaaaaaggc gatatgttgc tggacggttt ccgtcaactg gcgcgggaat
    35281 atcccgatct ggtacaggaa gcgcgtggca aagggatgtt gatggcgatt gagtttgttg
    35341 ataacgaaat cggctataac tttgccagcg agatgttccg ccagcgcgtc ctggtggccg
    35401 gaacgctcaa taacgccaaa acgatccgca ttgaaccgcc actgacactg accattgaac
    35461 agtgtgaaca ggtgatcaaa gcggcacgta aggcgctggc ggccatgcga gtcagtgtcg
    35521 aagaagcgta gtatcaaatc ggatggcgat tcgacgtcgc catccgatct ttttatacga
    35581 tacgtacgcc cgcaggcatc atccgctccg gcgttaacag cacgctttca ctgccgtcat
    35641 cggtttccgc acacaacagc atgcattccg acgtttcacc gcgcattttc gctttttgca
    35701 gattgcataa gaccaccacc gttttcccca tcagctcttc ttcgctgtag tacggcacca
    35761 gactggtcac ggtttgcagc gttttttcac ccacatcaac ctgtacgatg tacagcttgt
    35821 cggcgttttc atggcgtttc acttccacaa tctttccgac acgcatttcc agacgtgcaa
    35881 aatcagcgta agccacggtt tccattgttt cctcccttcg ggtaaaattt tactaaatta
    35941 tagcaaaagt ttttctcaat cctgtaggct aaaactggag aatgcaggcg tgatcacatt
    36001 cgtgagccgc tgtgttaccg ttacagcgtc aaagaaacgc gctttattta ctgaaaacag
    36061 gtgacccgat aagcacttcc tctacaatgg gggcgcacat cagggaaagt aaaaaaggta
    36121 aacatggcaa cactaaaaga catcgcaatc gaagctggcg tatccctggc gacagtatcc
    36181 agggtcttga atgacgatcc gacattgaat gtgaaagaag agacgaaaca tcgcattctc
    36241 gagatcgccg aaaagctgga gtacaagacc agtagcgccc gtaaactcca gacaggcgca
    36301 gtcaaccaac accatatttt ggctatctac agctaccaac aggagctgga gatcaacgat
    36361 ccttactatc tggcgatccg ccacggcatt gaaactcagt gcgaaaagct ggcgatcgaa
    36421 ctgaccaact gttatgaaca caacggctta ccagacatta aaaacgtcac cggtatttta
    36481 attgtcggca aacccacgcc cgccctgcgc gccgccgcct gtgcgttgac cgacaatatc
    36541 tgctttatcg actttcacga acccggcagc ggttacgatg cggtggatat cgatctggca
    36601 cgtatcagta aagaaatcat cgatttctat atcgatcagg gcgttaatcg tattggtttt
    36661 attggcggtg aagacttgcc tggcaaggcg gatattcgtg aggtcgcctt tgtggaatat
    36721 ggccgactga aacacgtggt acgcgaagag gatatctggc gcggcggttt ttccagttca
    36781 tcgggctatg aactggcaaa acagatgctg gcgcgggaag actatccgaa ggcactgttt
    36841 gttgcttccg attccattgc tatcggcgta ctgcgggcaa ttcatgaacg tggcctgaac
    36901 atcccacagg atatttcgct catcagcgtt aacgatatcc ccaccgcgcg atttaccttt
    36961 ccgccgctct ccaccgtgcg catccattcc gagatgatgg gaagtcaggg cgttaacctg
    37021 gtgtatgaaa aagcccgcga tggtcgcgcg ctgccgctgt tgatcttcgt tcccagcaaa
    37081 ttaaaactgc gcggcacgac ccgttaaatc cccttacaca ctgtccggca atcgtttttg
    37141 ccggacagtg ctgccgttta ttttcgtgat ccagttaaag taaatgcatt tacctgctac
    37201 tttttagtaa aaattttact aaactcccca gcaattacac aaactaccat caccataaat
    37261 ggttccgatt tctctctacc gggaggccct atgaatcgct gggaaaacat tcagctcacc
    37321 cacgaaaacc gacttgcgcc gcgtgcgtac tttttttcat atgattctgt tgcgcaggcg
    37381 cgtacctttg cccgcgaaac gagcagcctg tttctgccct taagcggtca gtggaatttc
    37441 cacttttttg accatccgct gcaagtgcca gaagccttca cctctgagtt aatggctgac
    37501 tgggggcata ttaccgtccc cgccatgtgg caaatggaag gtcacggcaa actgcaatat
    37561 accgacgaag gttttccgtt ccctatcgat gtgccgtttg tccccagcga taacccaacc
    37621 ggtgcctatc aacgtatttt caccctcagc gacggctggc agggtaaaca gacgctgatt
    37681 aaatttgacg gcgtcgaaac ctattttgaa gtctacgtta acggtcagta tgtgggtttc
    37741 agcaagggca gtcgcctgac cgcagagttt gacatcagcg cgatggttaa aaccggcgac
    37801 aacctgttgt gtgtgcgcgt gatgcagtgg gcggactcta cctacgtgga agaccaggat
    37861 atgtggtggt cggcggggat tttccgcgat gtttatctga tcggaaaaca actcacgcat
    37921 attaacgatt tcaccgtgcg caccgacttt gacgaagcct attgcgatgc cacgctttcc
    37981 tgcgaagtgg tgctggaaaa tctcgccgcc tcccctgtcg taacgacgct ggaatatacc
    38041 ctgtttgatg gggaactcgt ggtgcacagc agcgccattg atcatttggc aattgaaaaa
    38101 ctgaccagcg ccagctttgc ttttactgtc gaacagcccc agcaatggtc agcagaatcc
    38161 ccttatcttt accatttggt catgacgctg aaagacgccg acggcaacgt tctggaagtg
    38221 gtgccacaac gcgttggctt ccgtgatatc aaagtgcgcg acggtctgtt ctggatcaat
    38281 aaccgttatg tgatgctgca cggcgtcaac cgtcacgaca acgatcatcg caaaggccgc
    38341 gccgttggga tggatcgcgt cgagaaagat ctccagttga tgaaacagca caacatcaat
    38401 tccgtgcgta ccgctcacta cccgaacgat ccgcgttttt acgaactgtg tgatatctac
    38461 ggcctgtttg tgatggcgga aaccgacgtc gaatcgcacg gctttgctaa cgtcggcgat
    38521 atcagtcgta ttaccgacga tccgcagtgg gaaaaagtct acgtcgagcg cattgttcgc
    38581 catattcacg cgcagaaaaa ccatccgtcg atcatcatct ggtcgctggg caatgaatcc
    38641 ggctatggct gtaacatccg cgcgatgtac catgcggcga aggtgctgga tgacacgcga
    38701 ctggtgcatt acgaagaaga tcgcgatgct gaagtggtcg atattatttc caccatgtac
    38761 acccgcgtgc cgctgatgaa tgagtttggt gaatacccgc atccgaagcc gcgcatcatc
    38821 tgtgaatatg ctcatgcgat ggggaacgga ccaggtgggc tgacggagta ccagaacgtc
    38881 ttctacaagc acgattgcat tcagggacat tatgtctggg aatggtgcga ccacgggatc
    38941 caggcgcagg atgacaatgg caacgtctgg tataaattcg gcggcgacta cagcgactat
    39001 cccaacaact ataacttctg tcttgatggt ttgatctatt ccgatcagac gccgggacca
    39061 ggactgaaag agtacaaaca ggttattgcg ccggtaaaaa tccacgcgct ggatctgact
    39121 cgcggcgagt tgaaagtcga aaataaactg tggtttacca cgcttgatga ctacaccctg
    39181 cacgcagagg tgcgcgccga aggtgaaaca ctcgcgacgc agcagattaa actgcgcgac
    39241 gttgcgccga acagcgaagc ccccttgcag atcacgctgc cgcagctgga cgcccgcgaa
    39301 gcgttcctca acattacggt gaccaaagat tcccgcaccc gctacagcga agccgggcat
    39361 tctatcgcca cttatcagtt cccgctgaag gaaaacaccg cgcaggcagt gcctttcgca
    39421 ccaaataatg cgcgtccgct gacgctggaa gacgatcgtt tgagctgcac cgttcgcggc
    39481 tacaacttcg cgatcacctt ctcaaaaatg agtggcaaac cgacatcctg gcaggtaaat
    39541 ggcgagtcgt tgctgacccg cgagccaaag atcaacttct tcaagccgat gatcgacaac
    39601 cacaaacagg agtacgaagg gctgtggcaa ccgaatcatt tgcagatcat gcaggaacat
    39661 ctgcgcgact ttgccgtgga acagagcgat ggtgaagtgt tgatcatcag ccgcacggtt
    39721 attgccccac cggtgtttga cttcgggatg cgctgcacct acatctggcg catcgctgcc
    39781 gatggccagg ttaacgtggc gctttccggc gagcgttacg gcgactatcc gcacatcatt
    39841 ccgtgcatcg gtttcaccat ggggattaac ggcgaatacg accaggtggc gtattacggt
    39901 cgtggaccgg gcgaaaacta cgccgacagc cagcaggcta acatcatcga tatctggcgc
    39961 agcaccgtcg atgccatgtt cgagaactat cccttcccac agaacaacgg caaccgtcag
    40021 catgtccgct ggacggcact gactaaccgc cacggcaatg gtctgctggt ggttccgcag
    40081 cgcccaatta acttcagtgc ctggcactac acccaggaaa acatccacgc tgcccagcac
    40141 tgtaacgagc ttcagcgcag tgatgacatc actctgaatc tcgaccacca gctgcttggc
    40201 ctcggctcca actcctgggg cagcgaggtg ctggactcct ggcgcgtctg gttccgtgac
    40261 ttcagctacg gctttacgtt gctgccggtt tctggcggag aagctaccgc gcaaagcctg
    40321 gcgtcgtatg agttcggcgc agggttcttt tccacgaatt tgcacagcga gaataagcaa
    40381 tgaggatcat cgataactta gaacagttcc gccagattta cgcctctggc aagaagtggc
    40441 aacgatgcgt tgaagcgatt gaaaatatcg acaacattca gcctggcgtc gcccactcca
    40501 tcggtgactc attgacttac cgcgtggaga cggactccgc gaccgatgcg ctatttaccg
    40561 ggcatcgacg ctattttgaa gtgcattact acctgcaagg gcagcaaaaa attgaatatg
    40621 cgccgaaaga gacattacag gtagtggaat attatcgtga tgaaactgac cgtgaatatt
    40681 taaaaggctg cggagaaacc gtagaggtcc acgaagggca aatcgttatt tgcgatatcc
    40741 atgaagcgta tcggtttatc tgcaataacg cggtcaaaaa agtggttctc aaagtcacca
    40801 tcgaagatgg ttatttccat aacaaataac aactacggcg gcaaaaggag tttgccgcca
    40861 ccgctaccct actcattttc ggagatgtgt tatgtctgat accaaacgta atacaatcgg
    40921 caaattcggc ttgctctcgc tgacttttgc cgccgtttac agctttaaca acgttatcaa
    40981 taataatatt gagcttggac tggcctcggc accgatgttt ttcctcgcga cgatttttta
    41041 ttttattccc ttctgtctga tcatcgcaga atttgtttcg ttaaataaaa actcagaagc
    41101 cggtgtctac gcgtgggtaa aaagctcgct gggcggacgt tgggcattta ttactgccta
    41161 tacctactgg ttcgtaaacc tgttcttttt cacctcgctg ttaccgcgcg ttattgctta
    41221 tgcttcgtat gcattcctcg gctacgaata tattatgacg ccggttgcca ccaccattat
    41281 cagtatggtg ctgttcgcct tctccacctg ggtttctacc aatggggcga aaatgctggg
    41341 gccaattacc tccgtcactt caacgctgat gctgctgtta acgctctcct acattttact
    41401 ggcaggtacg gcactggttg gcggcgtaca gcctgctgac cccatcactg ttgacgcgat
    41461 gatcccaaac tttaactggg cgttcctcgg cgttaccacc tggatcttta tggccgcagg
    41521 tggcgcggag tccgtcgcgg tgtacgttaa cgacgtcaaa ggcggttcga aatcgttcgt
    41581 taaagtgatc atcctcgccg ggatttttat tggcgtactg tactctgtct cctcggtact
    41641 gattaacgtc ttcgtcagca gcaaagagtt gaagtttacc ggcggatcgg tacaggtatt
    41701 ccacggcatg gcggcgtatt ttggtctacc ggaagcactg atgaatcgct ttgtcggtct
    41761 ggtgtccttt accgcgatgt tcggttccct gctgatgtgg acggcaacgc cggtgaaaat
    41821 tttcttctcc gaaattccgg aaggcatctt tggtaagaaa accgtcgaac tgaacgaaaa
    41881 cggcgttccg gcgcgcgcag cgtggatcca gttcctgatc gtcatcccgc tgatgattat
    41941 cccaatgctc ggttccaata ccgtgcagga tctgatgaat actattatta atatgactgc
    42001 cgcagcgtcc atgcttccgc cgttattcat catgctggct tacctgaatt tacgcgccaa
    42061 attagatcac ctgccacgcg atttccgtat gggttcccga cgcaccggta ttatcgttgt
    42121 ttcaatgctg attgcgatat ttgctgtagg gtttgtcgct tcgacattcc cgactggcgc
    42181 gaatattctg accatcattt tttataacgt cggcggtatt gttatcttcc tcggctttgc
    42241 gtggtggaaa tacagtaaat atataaaggg attaacggct gaagagcgcc atattgaagc
    42301 gacgccagcc agcaatgttg attaagcaga ataataaaaa atgagcaggc attttccctc
    42361 ttcctggatg gggagaggga aaat
//
LOCUS       DAPHCV010000029        41528 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.029, whole genome shotgun sequence.
ACCESSION   DAPHCV010000029 DAPHCV010000000
VERSION     DAPHCV010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.029"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            18..836
                     /locus_tag="RH033_001600"
     CDS             18..836
                     /locus_tag="RH033_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HDV2990799.1"
                     /translation="MPCIGNAQEIKIDESWLHQSLNVIGRTDSRFGPRLTNDLYPEYT
                     VAGRKDWFDFYGYVDLPKFFGVGSHYDVGIWDEGSPLFTEIEPRFSIDKLTGLNLAFG
                     PFKEWFIANNYVYDMGDNQSSRQSTWYMGLGTDIDTGLPIKLSANIYAKYQWQNYGAA
                     NENEWDGYRFKIKYSIPLTNLFGGRLVYNSFTNFDFGSDLADKSHNNKRTSNAIASSH
                     ILSLLYEHWKFAFTLRYFHNGGQWNAGEKVNFGDGPFELKNTGWGTYTTIGYQF"
     gene            complement(1054..3486)
                     /gene="metL"
                     /locus_tag="RH033_001601"
     CDS             complement(1054..3486)
                     /gene="metL"
                     /locus_tag="RH033_001601"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HDV2990800.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISCNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLTH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(3489..4649)
                     /gene="metB"
                     /locus_tag="RH033_001602"
     CDS             complement(3489..4649)
                     /gene="metB"
                     /locus_tag="RH033_001602"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HDV2990801.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQSDEQALRAALAENPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEHTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            4926..5243
                     /gene="metJ"
                     /locus_tag="RH033_001603"
     CDS             4926..5243
                     /gene="metJ"
                     /locus_tag="RH033_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HDV2990802.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            5303..5911
                     /locus_tag="RH033_001604"
     CDS             5303..5911
                     /locus_tag="RH033_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HDV2990803.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGELSVEQQQK
                     LAQTAKRYLGKPYDSSFSWSDDRQYCSEVVWKVYQNALGMRVGKQQKLKEFDLSNPQV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(5972..6184)
                     /gene="rpmE"
                     /locus_tag="RH033_001605"
     CDS             complement(5972..6184)
                     /gene="rpmE"
                     /locus_tag="RH033_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDV2990804.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            6387..8585
                     /gene="priA"
                     /locus_tag="RH033_001606"
     CDS             6387..8585
                     /gene="priA"
                     /locus_tag="RH033_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDV2990805.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVLVSNVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRKNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            8741..9766
                     /gene="cytR"
                     /locus_tag="RH033_001607"
     CDS             8741..9766
                     /gene="cytR"
                     /locus_tag="RH033_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HDV2990806.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            9858..10817
                     /gene="ftsN"
                     /locus_tag="RH033_001608"
     CDS             9858..10817
                     /gene="ftsN"
                     /locus_tag="RH033_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HDV2990807.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQGQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            10910..11440
                     /gene="hslV"
                     /locus_tag="RH033_001609"
     CDS             10910..11440
                     /gene="hslV"
                     /locus_tag="RH033_001609"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HDV2990808.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            11450..12781
                     /gene="hslU"
                     /locus_tag="RH033_001610"
     CDS             11450..12781
                     /gene="hslU"
                     /locus_tag="RH033_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HDV2990809.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTITIDADYVSKHLDALVADEDLSRFIL"
     gene            12848..13774
                     /gene="menA"
                     /locus_tag="RH033_001611"
     CDS             12848..13774
                     /gene="menA"
                     /locus_tag="RH033_001611"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDV2990810.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            13867..14352
                     /gene="rraA"
                     /locus_tag="RH033_001612"
     CDS             13867..14352
                     /gene="rraA"
                     /locus_tag="RH033_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HDV2990811.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYTDNTGIILSEDPLDI
                     E"
     gene            complement(14437..14682)
                     /gene="zapB"
                     /locus_tag="RH033_001613"
     CDS             complement(14437..14682)
                     /gene="zapB"
                     /locus_tag="RH033_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HDV2990812.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            15108..15953
                     /gene="glpF"
                     /locus_tag="RH033_001614"
     CDS             15108..15953
                     /gene="glpF"
                     /locus_tag="RH033_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HDV2990813.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            15976..17484
                     /gene="glpK"
                     /locus_tag="RH033_001615"
     CDS             15976..17484
                     /gene="glpK"
                     /locus_tag="RH033_001615"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDV2990814.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            17655..18665
                     /gene="glpX"
                     /locus_tag="RH033_001616"
     CDS             17655..18665
                     /gene="glpX"
                     /locus_tag="RH033_001616"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HDV2990815.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            18762..19508
                     /gene="fpr"
                     /locus_tag="RH033_001617"
     CDS             18762..19508
                     /gene="fpr"
                     /locus_tag="RH033_001617"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HDV2990816.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEIE
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLERFKNMVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRLPALIESGELESAVGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(19513..19941)
                     /gene="uspD"
                     /locus_tag="RH033_001618"
     CDS             complement(19513..19941)
                     /gene="uspD"
                     /locus_tag="RH033_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HDV2990817.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(19968..20267)
                     /locus_tag="RH033_001619"
     CDS             complement(19968..20267)
                     /locus_tag="RH033_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HDV2990818.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(20479..20919)
                     /locus_tag="RH033_001620"
     CDS             complement(20479..20919)
                     /locus_tag="RH033_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDV2990819.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            21020..21619
                     /locus_tag="RH033_001621"
     CDS             21020..21619
                     /locus_tag="RH033_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HDV2990820.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQAYMAAVIRTLTPLMTKTQSQKKLQALLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            21727..22494
                     /gene="tpiA"
                     /locus_tag="RH033_001622"
     CDS             21727..22494
                     /gene="tpiA"
                     /locus_tag="RH033_001622"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDV2990821.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(22549..23304)
                     /gene="cdh"
                     /locus_tag="RH033_001623"
     CDS             complement(22549..23304)
                     /gene="cdh"
                     /locus_tag="RH033_001623"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HDV2990822.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(23411..24400)
                     /gene="sbp"
                     /locus_tag="RH033_001624"
     CDS             complement(23411..24400)
                     /gene="sbp"
                     /locus_tag="RH033_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HDV2990823.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKVFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(24720..25682)
                     /gene="pfkA"
                     /locus_tag="RH033_001625"
     CDS             complement(24720..25682)
                     /gene="pfkA"
                     /locus_tag="RH033_001625"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDV2990824.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIELL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(25863..26765)
                     /gene="fieF"
                     /locus_tag="RH033_001626"
     CDS             complement(25863..26765)
                     /gene="fieF"
                     /locus_tag="RH033_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HDV2990825.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(26914..27414)
                     /gene="cpxP"
                     /locus_tag="RH033_001627"
     CDS             complement(26914..27414)
                     /gene="cpxP"
                     /locus_tag="RH033_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HDV2990826.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            27564..28262
                     /gene="cpxR"
                     /locus_tag="RH033_001628"
     CDS             27564..28262
                     /gene="cpxR"
                     /locus_tag="RH033_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HDV2990827.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALSLNPGRQEATFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            28259..29632
                     /gene="cpxA"
                     /locus_tag="RH033_001629"
     CDS             28259..29632
                     /gene="cpxA"
                     /locus_tag="RH033_001629"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HDV2990828.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(29738..30412)
                     /gene="yiiM"
                     /locus_tag="RH033_001630"
     CDS             complement(29738..30412)
                     /gene="yiiM"
                     /locus_tag="RH033_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HDV2990829.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTDLGLEGDEQ
                     AEKKIHGGSDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGLVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(30561..31544)
                     /gene="kdgT"
                     /locus_tag="RH033_001631"
     CDS             complement(30561..31544)
                     /gene="kdgT"
                     /locus_tag="RH033_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HDV2990830.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(31804..32424)
                     /gene="sodA"
                     /locus_tag="RH033_001632"
     CDS             complement(31804..32424)
                     /gene="sodA"
                     /locus_tag="RH033_001632"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HDV2990831.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     NLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPIVGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            32710..33744
                     /gene="rhaT"
                     /locus_tag="RH033_001633"
     CDS             32710..33744
                     /gene="rhaT"
                     /locus_tag="RH033_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HDV2990832.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGMGMAN"
     gene            complement(33741..34607)
                     /gene="rhaR"
                     /locus_tag="RH033_001634"
     CDS             complement(33741..34607)
                     /gene="rhaR"
                     /locus_tag="RH033_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HDV2990833.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQIIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(34663..35499)
                     /gene="rhaS"
                     /locus_tag="RH033_001635"
     CDS             complement(34663..35499)
                     /gene="rhaS"
                     /locus_tag="RH033_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HDV2990834.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQEQDGQYPSHWRVNHSVLQQVRQLVAQMEQQEEENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADLFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            35787..37256
                     /gene="rhaB"
                     /locus_tag="RH033_001636"
     CDS             35787..37256
                     /gene="rhaB"
                     /locus_tag="RH033_001636"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HDV2990835.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFKNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRNIYQRSGIQFLPFNTLYQLHALTEQQPELIPHIAHALLM
                     ADYFSYRLTGEMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSHAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNALLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            37253..38512
                     /gene="rhaA"
                     /locus_tag="RH033_001637"
     CDS             37253..38512
                     /gene="rhaA"
                     /locus_tag="RH033_001637"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HDV2990836.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKSNQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLESAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLENVRAYEKEILSRRG"
     gene            38697..39521
                     /gene="rhaD"
                     /locus_tag="RH033_001638"
     CDS             38697..39521
                     /gene="rhaD"
                     /locus_tag="RH033_001638"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HDV2990837.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTHEAVPTTELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            39531..39845
                     /gene="rhaM"
                     /locus_tag="RH033_001639"
     CDS             39531..39845
                     /gene="rhaM"
                     /locus_tag="RH033_001639"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HDV2990838.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPTNPDNSPVSSELQEVFY
                     LP"
     gene            complement(39886..41280)
                     /locus_tag="RH033_001640"
     CDS             complement(39886..41280)
                     /locus_tag="RH033_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HDV2990839.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLTLEQRMALLEERLEAAEM
                     RAAKAESQVKQLQTQQAAEIREIKAAQGNTPVNGQATAESAKKNSTSPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKLDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPENGVVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYTIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKKHGDDT
                     DDRYGARVRFKYFF"
BASE COUNT        10223 a        10851 c        10633 g         9821 t
ORIGIN      
        1 ctttatccat tctattaata ccttgcattg gaaacgctca ggaaattaaa attgatgaaa
       61 gctggttaca tcaaagcttg aatgtcattg gtcgcacaga ctctcgcttt ggcccaagac
      121 tgactaacga cctctaccct gaatatactg tagcaggaag aaaagactgg tttgattttt
      181 atggttatgt tgatctacca aaattctttg gcgtcggcag tcactatgat gttgggatct
      241 gggatgaggg ctcaccacta tttacggaaa tagaacctcg gttttccatt gacaaattga
      301 ccggattaaa tcttgcgttc ggcccattta aagaatggtt cattgcaaac aactatgtct
      361 atgatatggg tgacaaccag tcatcccggc aaagtacatg gtatatgggg cttggtacag
      421 atatcgacac gggtctacca attaagcttt ctgccaatat atacgccaag tatcagtggc
      481 aaaactatgg tgccgctaat gaaaatgaat gggacggata tcgattcaaa ataaaatata
      541 gcatccctct tacaaattta ttcggagggc gattggtata caatagtttt actaactttg
      601 attttggctc cgatcttgcg gacaagtcac acaataataa acgaaccagt aatgctattg
      661 cttcaagcca tatcctttcc cttctatatg aacactggaa atttgcattt acactacgtt
      721 attttcacaa cggtggacaa tggaatgcgg gagagaaggt taacttcgga gatggtccat
      781 ttgaattgaa aaatacagga tggggaacct atactactat tggttatcaa ttttaaatta
      841 ggtgacattt tagatattgt tacttaatgt cattttgtaa ttgtcattaa taacttcacc
      901 attcactctg ttacttcatc gctcaacaca aaagccccct tattaccggg ggcactgacc
      961 aaaggcaaaa tgacaatcaa tcaccacttc cccaggaaga ggtttagggg tgacaacaaa
     1021 agctggcaac tgccagcttt tgttcaataa tcactacaac aactgcgcca gccggttaat
     1081 atccgactga atcgccccgg cggtgacgtc gcgccctgca cctggcccgc ggatcaccag
     1141 cgggttgtcg cgataccagc ggctttcgat ggcaaagacg ttatcgcatg gcagtagtga
     1201 tgccagcgga tggtcttcac gcaccgcttc cacgcccaca cgcgctttgc cattggcatc
     1261 gaaacgcgcc acgtagcgca gcaccagccc catttcgcgg gccgcttcca gtcgttgcac
     1321 catctgctcg ttaagttcat cgccattttc aaagaaatgg tcgatgctgc cgccttcgca
     1381 atgggcaggt accagtgatt caacacgtac ctggtccggt tcaatgttgt aacccgcttc
     1441 acgcgccaga atcaccagct tgcgcatcac gtctttgccg gaaaggtcat cacgcgggtc
     1501 aggctcggtt aagccctgct gccacgcctg atccaccagt tcggtaaacg gcacgctacc
     1561 gtcgaattgc aggaacagcc aggagagcgt gccagagaag atcccactga tcgacaaaat
     1621 ggtatcgccg ctgtcgatca gatcgcgcac ggtatggttg atcggcaaac ccgcaccgac
     1681 agtggcattg tacagccagt gacgcccggt tttttcgaag gcgtcgtgga tctggcgata
     1741 tttattgctg tcgctggctc ctgccagttt gttggcactg atgacgtgga aaccgtggct
     1801 ggcgaaatca agatactggt cagcgagctg ctggctggcg gtaacgtcca gcaccactaa
     1861 atcatcatac gggtgggcgc gcatccacag gaacagcgac tcttcatcct gctcgaccgc
     1921 ttcatcgttg aagaaggcta acgcgcggct ggcgtccagc ccgtcatagc tcaacaggct
     1981 gcggcggctg tccaccacgc ctgccagcac aaattcaaag ccggtacgcg ccgaaagcgt
     2041 gctctgctca cgggcgaaca gttccagcca gcgggaaccg atattgccct tgccgaacaa
     2101 taccaggccg atgcgttttt ctgcgcggaa gacggactga tgcagcccct gaatcaggct
     2161 ttcggtcggg ccggtgcgca gtactgccac caggctgatg ccgtcatcgg actgccaggt
     2221 aaattcgacc ggctggcctt tcagttgctg ccagaagcgg tggcaatgca gcgggttacg
     2281 ggtgacgcct gccccaacca tcgccaccag cgccagcccc tgacgcaggc gcagttcgcc
     2341 aggtaatccc gcttcgtcga ggattttcag cgcactgtcg gccacttctg aggtgtagca
     2401 aaattgcagc aactggcgat cgttatgcac gccaaccgcc agtgggcgca cctgagcacg
     2461 tttcaggatc tggtcgatct ctttatgcgt cagtttgaaa tcctggctgg cgggcacctg
     2521 aaactcaatc aagcagacat catcatggct ggtgacaata cgcgcaccag taccggaggc
     2581 cagcacgcgt tcaatgcgcg tggaaccttg atccggcgtg tagctacagc gcagttgcag
     2641 gtcgatttcg ctgccagaaa cgggctgtaa agtacgggca tgaagaacgg gagccgccag
     2701 acgcgccagt tcgctggctt catccagacg cagcaacggc agcaggcagg catctttcac
     2761 tttacgcggg tcggcgctat ataccccggc gacgtcgctc cagatggtta cgcgagaaac
     2821 acccgccagc gcaccgattt gtgtcgcgga atagtcggaa ccgttacgcc ccagcagcac
     2881 cgtttcaccg gcgttgttac agctgataaa cccggtcacc accagacgtt tgcccggatg
     2941 ttgtaccagt agctgttgca gcaacgggta agaaagtcct tcatcaacct gcggttgcgc
     3001 agcgcgttca gcgcgtaaaa actcgcgggc atcaagccag gccgctggca gcccttgttg
     3061 attaagcacc gcagacatca ggcgcgccga ccacacttca ccgtggccca ccacttccgc
     3121 atacaccgcg tcgttaatac cgctgtcgag caacgccgcc aggcgctcaa gatcgctgac
     3181 aaaagcgcta atgaggctat cggcttcttc ggcgggtaac aggccgctaa tcagatcgca
     3241 ctgatagcga cgcagcgttt gttgaacctg atgcgcagag agacgatcgg tctggcttag
     3301 tttcaaccag ttaatcaact ggttagtggt gctaccggcg gcggaaacca ccatcatatc
     3361 gtcaggctga gagtactccg ccataatgcc cgcgacacgc aaataacact tcacatcagc
     3421 cagactactg ccaccaaatt tatgcagttg acgacctttc gcccctgcct gcgcaatcac
     3481 actcattttt accccttgtt tgcagcccgg aagccatttt ccaggtcggc aattaaatct
     3541 tcgccatctt caataccggt ggagatacgc agcagcgtct cggagatccc ggcggcagca
     3601 cgcgcttctg gtgccatgcc tgcatgtgtc atggttgcgg cgtgagagat taaactttcc
     3661 actcccccta atgattccgc cagcgtaaac aacgacagcc cgcccaggaa acgacgcagc
     3721 gtgtgctcat cgccatccag ttcaaaactc aacattgcgc caaagccttt ttgctggcgc
     3781 gcggcaattt catgcccctg attttccggc agcgacgggt gatacagttt tttcaccaac
     3841 ggctgggttt gcagatattt cacaatcgcc tgcgcgttgc gctgggccag ctccatacgc
     3901 ggcaccagtg ttcgcaaccc gcgcagcagc agatagctgt caaacgcgcc gcccgtcacg
     3961 ccaatattgt ttgcccacca ggcaagttca gtgacaacgt ctggatcttt agcaatcacc
     4021 acgcctgcca ccacgtctga gtgaccgttc agatatttcg tacatgaatg caacaccaga
     4081 tcggcaccta atgccagcgg attttgtaat gccgggctta agaaggtgtt atccaccaca
     4141 ctcaccgccc cgacttccct tgccagatgg cagattttcg caatatccac gacgcgtaac
     4201 aatggattac ttgggctttc taccagtacc agtttggggt tttctgccag cgctgcccgt
     4261 aatgcctgtt catcgctttg atcaacaaac aacacgcgat agcaaccgcg tttcgccaga
     4321 ctgtcgaaca ggcgatagct gccgccatag cagtcgtgcg gcgcaaccag cagatcgccc
     4381 ggtttcaaaa agacggtcgt taccaggtga atcgcggaca tgccggtatt agtaagtacg
     4441 gcaccagcac cgccttccag ttctgccagc gcacgctgaa ccacatcgcg cgttgggttg
     4501 ccgcgccgcg agtaatcatg cgcgcgcggt tcattaaatc cggtaaagtt ataggtgctg
     4561 gaaagatgga tcggtgggac aacgcaacca tactgttcgt cgtcatttaa cccgctacgc
     4621 actgcgatgg tggcctgttt acgcgtcatg tgatgaagtt ccctgggctt ggtcggtgaa
     4681 atgtcaggca ccagagtaaa cattgtgtta atggacgtca atacatctgg acatctaaac
     4741 ttctttgcgt atagattgag caaatcccaa atagccgtta aaattatatg cattatcacg
     4801 ccgacaggtg cattacacga tgtcacggta acgcctgtac ggtaaactat gcgggtttac
     4861 ggtcagtacc catattaatt gtgtggtctg gcctcaattt attgacgaag aggattaagt
     4921 atctcatggc tgaatggagc ggcgaatata tcagcccata cgctgagcac ggcaagaaga
     4981 gtgaacaagt caaaaaaatt acggtttcca ttcctcttaa ggtgttaaaa atcctcaccg
     5041 atgaacgcac gcgtcgtcag gtgaacaacc tgcgtcacgc gaccaacagc gagctgctgt
     5101 gcgaagcgtt tctgcatgcc tttaccgggc aacctttgcc ggatgatgcc gacctgcgta
     5161 aagagcgcag cgacgaaatt ccggaggcgg caaaagagat catgcgtgag atggggatta
     5221 acccggagac gtgggaatac taaagcggaa aagagcggcg cggagttaac cagccgcttt
     5281 tttcatacat ggacgtttaa cgatgaaaat caggctactg attctcagcc tgctggtttc
     5341 tgtgcctgcc tttgcctggc agccgcaaac tggcgacatc atctttcaaa tctctcgctc
     5401 atcgcaaagt aaagcgatcc aactggcgac ccattccgat tacagccaca ccggtatgct
     5461 ggtgatacgc aacaaaaagc cctacgtttt tgaagcggtc ggcccggtaa aatacacccc
     5521 actcaagcag tggatcgccc atggtgaaaa gggcaaatac gttgttcgcc gcgttgaagg
     5581 cgaactgagt gttgaacaac agcaaaaact ggcgcaaacg gcaaaacgtt atctcggtaa
     5641 accgtatgac tccagcttct cgtggagcga cgatcgccag tactgttcgg aagtggtttg
     5701 gaaggtttat cagaacgcgc tgggaatgcg cgtgggcaaa cagcaaaagt tgaaagagtt
     5761 cgaccttagt aacccgcagg ttcaggcaaa gctcaaagaa cgctacggca aaaatattcc
     5821 gcttgaggaa acggtggtct cgccacaagc cgttttcgat gcgccacaac tcaccacggt
     5881 cgccaaagaa tggccgctgt tttcgtggta atacgaccaa aattcaggca caaaaaaagc
     5941 gccgtgcggc gcttttttcg gaaatccggt cttatttgct gcccgggatg ttgaaacgct
     6001 tgttgaagcg gtcaacacgg ccaccggtag caacatcacg ctgtttgcca gtgaagaacg
     6061 ggtggcactt gctgcacacg tcgaggttca ggtcatgacc aacggtggag cggattttca
     6121 ttacgttacc gcaagagcag ctagcagtaa tttcttcgta tttcgggtga atatcttttt
     6181 tcatgggaaa acctcggttt aaggccgcgt cgctcttcca gccctaacgc cagacaccac
     6241 gcgatgttaa aagtatagct tcaatacgat catttcgtat gaaggcgcga aatcatacag
     6301 aaattaacca gcgtatgcaa actgatccgc actcttctac ggcaatgtgt atactaaccc
     6361 accgaatttc aagtcaggat gatgctatgc ccgttgccca cgttgccttg cccgttccgc
     6421 ttccccgtac ctttgactat ctgctgccag aaggcatgac ggtcaaagcc gggtgtcgcg
     6481 tgcgcgtgcc gtttggcaaa cagcaggagc gcatcggggt tgtggtgtta gttagcaatg
     6541 tcagcgagct gccgcttaac gagctgaaag cggtagttga agtgctggac gttgagccgg
     6601 tatttactca ctccgtctgg cgattactgc tctgggcggc agattactat catcatccga
     6661 ttggcgatgt gctgtttcat gccttgccga ttttactacg ccaggggcgg cctgcggcga
     6721 acgcgccgat gtggtactgg tttgccactg aacaaggtca ggcggtggat ctgaacagcc
     6781 tgaaacgctc acccaagcaa caacaggcgc tggcagcgtt acggcaaggc aaaatctggc
     6841 gcgaccaggt tgccacgctc gaatttaatg atgccgcgtt gcaggcgctg cgcaaaaaag
     6901 gtctgtgtga tttagcaagt gaaacaccag agtttagtga ctggcgaaag aattatgccg
     6961 tttctggtga gcggttgcga ttgaatactg aacaggccac cgccgttggc gcaattcata
     7021 gtgcggcgga taccttctct gcctggctgc tggcgggcgt taccggttcc ggtaaaacgg
     7081 aggtttatct cagtgtactg gaaaacgtgc tcgctcaggg caaacaggcg ctggtgatgg
     7141 tgccggaaat cggcctgacg ccgcaaacta tcgcccgttt tcgtgaacgt tttaatgcgc
     7201 cagtggaagt tctgcattcc ggcctgaacg acagcgagcg tctttcggcg tggttgaaag
     7261 cgaaaaatgg tgaggcggcg attgtgatcg gcacccgctc cgcgctgttt acgccgttta
     7321 aaaatctcgg tgtgattgtc attgatgaag agcacgacag ttcctacaag cagcaggaag
     7381 gctggcgcta tcatgcccgc gacctggcgg tttatcgtgc gcacagcgag caaatcccga
     7441 ttattcttgg ctccgcaaca cccgcactgg aaacgttatg caacgtccag cagaaaaaat
     7501 accgcctgct gcgcctgacg cgtcgggcgg ggaatgcgcg tccggcaatt caacacgtgc
     7561 tggatttaaa aggtcagaag gtgcaggcgg ggctggctcc ggcgttaatc acccgtatgc
     7621 gccagcattt acaggccgac aaccaggtta tcctctttct taaccgccgt ggctttgcgc
     7681 ctgccttgct gtgccacgac tgcggctgga ttgccgaatg tccacgttgc gatcactact
     7741 acacgctgca tcaggcgcaa cagcatttgc gctgtcacca ctgtgacagc caacgcccgg
     7801 tgccgcgcca gtgcccttcc tgcggttcca cgcacctggt gccggtgggg ctgggcaccg
     7861 aacagcttga acagacgctt gcgccgctgt tccctggtgt gcccatttct cgtatcgacc
     7921 gcgataccac cagccgcaaa ggagcgctgg aacaacaact ggcggaagtg catcgcggcg
     7981 gcgcgaggat cttgatcggc acgcaaatgc tggcgaaagg tcaccacttt ccggatgtga
     8041 cgctggtggc gttactggac gtagacggcg cgctgttttc tgccgatttt cgctcggcag
     8101 agcgtttcgc ccagctttac acccaggtcg ccggtcgtgc cgggcgtgcg ggtaaacagg
     8161 gcgaagtggt gctgcaaacg catcatccag aacacccgct attgcaaacg ttgctctata
     8221 aaggctatga cgcctttgcc gaacaggcac tggctgagcg acgcatgatg cagctgccgc
     8281 cgtggaccag ccatgtgatt gtgcgtgcgg aagatcataa caatcagcac gcgccgctgt
     8341 tcctgcaaca actgcgtaat ctcatcctct ccagcccgct ggcagacgac aaactgtggg
     8401 ttctcggtcc cgttccggca ctggcaccta aacgtggcgg ccgctggcgc tggcaaattt
     8461 tgttgcagca cccttcccgc gtgcgattac agcacatcat caatggtacg ctggcgctta
     8521 ttaatacaat accggattcc cgtaaggtga aatgggtgct ggatgtcgat cccatcgagg
     8581 gctaatgcgc tcacgatgcg aggcggatcg aaaaattcaa tattcatcac acttttcatg
     8641 aaaattctgt aaccgttttc acgcgctatc tgctaaaaat gttgccgatg tgaagtaaac
     8701 atggatgtag tacgcctgac gtgccaggcg aggagtgagt gtgaaagcga agaagcagga
     8761 aactgccgcg accatgaaag acgttgccct caaggcaaaa gtctctacag cgactgtctc
     8821 ccgagcatta atgaatcccg ataaagtctc ccaggccacc cgtaatcggg ttgaaaaagc
     8881 ggcccgggaa gtgggttatt taccgcagcc tatggggcgc aacgtcaagc gtaatgaatc
     8941 ccgcactatt ctggtgattg tcccggatat ctgcgatccc ttctttagcg aaattattcg
     9001 cggtatcgaa gttacggcgg caaatcacgg atatctggtg ctgattggcg actgtgcgca
     9061 tcaaaatcag caggaaaaaa cctttatcga tttgatcatc accaagcaaa ttgatggcat
     9121 gttgctgctg ggttcaaggc tgccgtttga tgccagcatt gaggaacagc gtaatctgcc
     9181 gccgatggtg atggcgaacg aatttgcacc ggaactggag ctgcctacag ttcatatcga
     9241 caatctgacc gccgcatttg atgcagtaaa ttatttatat gagcaagggc ataaacggat
     9301 tggctgtata gccggtcccg aagagatgcc gctgtgtcac taccgcctgc aaggctatgt
     9361 ccaggcgctg cgtcgctgcg gcattatggt tgatccgcaa tacatcgccc gtggcgactt
     9421 caccttcgaa gctggaagca aagcaatgca acagctgctt gatcttccac aaccgcctac
     9481 tgctgtcttc tgccatagtg atgtgatggc gctcggcgca ctttcgcagg caaaacgcca
     9541 ggggctaaaa gtaccggaag acctttccat aatcggtttt gataacatcg acctgacgca
     9601 attttgtgat ccgccgctga caaccatcgc gcagccgcgt tacgaaatcg gtcgggaagc
     9661 tatgctgtta ttgcttgatc aaatgcaggg gcaacacgtt ggcagtggct ctcgtttaat
     9721 ggactgcgaa cttatcatcc ggggatcaac acgcgcgtta ccttaaagta aacctttaag
     9781 actgccgtga ctggtcaaag gcccgccgct taagtaacat ggcgggctga cgaacgaata
     9841 aatacagcga aacgatagtg gcacaacgag attatgtacg ccgcagccaa ccggcacctt
     9901 cgcggcgaaa aaagagcacc tcacggaaaa agcaacgaaa tttgcctgcg gtttctcccg
     9961 ctatggtcgc tattgctgcc gccgttcttg tgacctttat cggtggtctg tacttcatta
    10021 cgcatcacaa gaaagaagag tccgagacgc tgcaaggcca gaaagtgacc ggaaacggac
    10081 taccaccaaa accagaagaa cgctggcgct acattaaaga gctggaaagt cgccagccgg
    10141 gtgtgcgtgc gcccacagaa ccttctgccg gtggtgaagt gaaaacgccg gagcagctga
    10201 caccagaaca acgtcagctt cttgaacaaa tgcaggccga tatgcgccag cagccaacgc
    10261 agctggttga agttccgtgg aatgagcaga cgccggaaca gcgtcagcaa acgctacagc
    10321 gccagcgtca ggcacagcaa ttagcggagc agcaacgcct ggctcagcag tcgcgtacga
    10381 ctgaacaaag ctggcagcag cagacgcgta cgtcgcaagc cgcaccggta caggcgcaac
    10441 cgcgccagtc taaaccggct tctactcagc agccgtacca ggatctgctg caaacccctg
    10501 cgcacacgac cgcgcagtcg aagccgcagc aagctgcgcc ggttgctcgt gccgctgacg
    10561 cgccaaaacc gacggcggag aaaaaagacg aacgccgctg gatggtgcag tgcggttcgt
    10621 tcagaggcgc ggaacaggca gagacggtac gtgctcagct ggcgtttgaa ggctttgact
    10681 cgaaaatcac caccaacaat ggctggaatc gtgtggtcat tggcccggtg aaaggcaaag
    10741 agaacgcaga cagcaccctc aatcgcttga agatggcggg tcatacaaac tgcattcggc
    10801 tcgccgccgg gggttgaaac cctcaaaatc ccccccatct ataattgcat tatgccccgt
    10861 acttttgtac ggggtttgta ctctgtattc gtaaccaagg ggtcagctcg tgacaactat
    10921 agtaagcgta cgccgtaacg gccatgtggt catcgctggt gatggtcagg ccacgttggg
    10981 caataccgta atgaaaggca acgtgaaaaa ggtccgccgt ctgtacaacg acaaagtcat
    11041 cgcgggcttt gcgggcggta ctgcggatgc ttttacgctg ttcgaactgt ttgaacgtaa
    11101 actggaaatg catcagggcc atctggtcaa agccgccgtt gagctggcaa aagactggcg
    11161 taccgatcgc atgctgcgca aacttgaagc actgctggca gtcgcggatg aaaccgcttc
    11221 gcttatcatc accggtaacg gtgacgtggt gcagccagaa aacgatctta ttgctatcgg
    11281 ctccggcggc ccttacgccc aggctgcggc gcgcgcgctg ttagaaaaca ctgaacttag
    11341 cgcccgtgaa attgctgaaa aggcgttgga tattgcaggc gacatttgca tctataccaa
    11401 ccatttccac accatcgaag aattaagcta caaagcgtaa ggatctccca tgtctgaaat
    11461 gaccccacgc gaaatcgtca gcgaactgga taagcacatc atcggccagg acaacgccaa
    11521 gcgttccgtg gcgattgctc tgcgtaaccg ctggcgtcgc atgcagctca acgaagagct
    11581 gcgccatgaa gtgaccccga aaaatatcct gatgatcggc ccgaccggtg tcggtaaaac
    11641 tgaaatcgcc cgtcgtctgg ctaagctggc gaatgcgccg ttcatcaaag ttgaagcgac
    11701 caaattcacc gaagtgggct atgtcggtaa ggaagtggat tctattattc gcgatctgac
    11761 cgatgccgcc gtgaaaatgg tacgtgtcca ggctatcgag aaaaaccgtt atcgcgctga
    11821 agaactggca gaagaacgta ttctcgacgt actgatccca ccggctaaaa acaactgggg
    11881 acagaccgaa cagcagcagg aaccgtccgc tgctcgtcag gcattccgca aaaaactgcg
    11941 tgaaggccag cttgatgaca aagaaatcga gatcgatctt gccgcagcac cgatgggcgt
    12001 tgaaattatg gctcctccgg gcatggaaga gatgaccagc cagctgcagt ccatgttcca
    12061 gaacctgggc ggtcagaagc aaaaagcgcg taagctgaaa atcaaagacg ccatgaagct
    12121 gctgattgaa gaagaagcgg cgaaactggt gaatccggaa gagctgaagc aagacgctat
    12181 cgacgctgtt gagcagcacg ggatcgtgtt tatcgacgaa atcgacaaaa tctgtaagcg
    12241 cggcgagtct tccggtccgg atgtttctcg tgaaggtgtt cagcgtgacc tgctgccgct
    12301 ggtagaaggt tgcaccgttt ccaccaaaca cgggatggtc aaaactgacc acattctgtt
    12361 tatcgcttct ggcgcgttcc agattgcgaa accgtctgac ctgatcccgg aactgcaagg
    12421 tcgtctgcca atccgcgttg agctgcaggc actgaccacc agcgacttcg agcgtattct
    12481 gaccgagccg aatgcctcta ttaccgtgca gtacaaagca ctgatggcga ctgaaggcgt
    12541 aaatatcgag tttaccgact ccggtattaa gcgcatcgcg gaagcggcat ggcaggtgaa
    12601 cgaatctacc gaaaacatcg gtgctcgtcg tttacatacc gttctggagc gtttaatgga
    12661 agagatttcc tacgacgcca gcgatttaag cggtcaaacc atcactattg acgcagatta
    12721 tgtgagcaaa catctggatg cgttggtggc agatgaagat ctgagccgtt ttatcctata
    12781 atcgcgttca atcattttca tcattgtttg atggggctga aaggccccat ttttattggc
    12841 gcgtattatg actgaacaac aaattagccg aactcaggcg tggctggaaa gtttacgacc
    12901 taaaaccctc cccctcgcct ttgctgcaat tatcgtcggg acggcgctgg catggtggca
    12961 aggtcacttc gatccgctgg tcgccctgct ggcactgatt accgccgggc tattacagat
    13021 cctttctaac ctcgccaatg attacggcga tgcggtaaaa ggcagcgata aacctgaccg
    13081 tatcggaccg ctacgcggca tgcaaaaagg ggtcattacc cagcaagaga tgaaacgggc
    13141 gctcattatt accgtcgtgc tcatctgtct ctccgggctg gcactggttg cagtggcgtg
    13201 ccatacactg gccgattttg tcggtttcct gattcttggc gggttgtcga tcattgccgc
    13261 tatcacctac accgtgggca atcgtcctta tggttatatc ggtctgggtg atatttccgt
    13321 actggttttc tttggctggt tgagcgtcat gggcagctgg tatttacagg ctcatacatt
    13381 gattccggcg ctgatccttc cggcgaccgc atgcggcctg ctggcaacgg cagtactgaa
    13441 cattaataac ctgcgtgata tcaatagcga ccgcgaaaat ggcaaaaaca cgctggtggt
    13501 gcgcttaggt gaagtgaacg cgcgtcgtta tcatgcctgc ctgctgatgg gctctctggt
    13561 gtgcctggcg ctgtttaatc tcttttcgct gcatagcctg tggggctggc tgttcctgct
    13621 ggcggcacca ttactggtga agcaagcccg ttatgtgatg cgggaaatgg acccggtggc
    13681 gatgcgaccg atgctggaac gcactgtcaa gggagcgtta ctgactaacc tgctgtttgt
    13741 tttagggata ttcctaagcc agtgggcagc ataactgaca aatatcaatt aacaattgat
    13801 gattttgcca acagcctaca tagcgcgata tactgaaaat tctcgcagca actgaatgtt
    13861 aagcctatga aatacgatac ttccgagctt tgtgacatct atcaagaaga tgttaacgtc
    13921 gtggaaccgc tgttctccaa ctttggcgga cgggcgtcgt ttggcggaca aataatcacg
    13981 gtaaaatgtt tcgaggacaa cgggttgctg tacgatctgc tcgaacagaa tggccgtggt
    14041 cgtgttcttg tcgtcgatgg cggtggttct gttcgtcgcg cactggtcga tgctgaactg
    14101 gcgcgtctgg cagtacaaaa tgaatgggaa ggtctggtca tttacggcgc ggtgcgtcag
    14161 gtagatgacc tggaagagct ggatatcggc atccaggcaa tggccgcaat tccggttggt
    14221 gccgctggcg aaggcattgg cgaaagcgat gtccgcgtca attttggcgg tgtaaccttc
    14281 ttctccggcg accatcttta caccgacaat accgggatta ttctttcaga agatccgctg
    14341 gatattgaat gataaaaaag gcaccgcaag gtgccttttt tctgcgttac ctgttggcct
    14401 acacagtaaa gaaattacgc gaaagatgaa gcgtaatcag acctcttcca tgcgacccag
    14461 cagggcctgc agacgttcct gccagccgtt ctgctgttct ttcagatggt tgttctcacg
    14521 ctccagctct tcgcgctgat gctgggcatt ttgaacttcc tgcgacagtg agttgttttt
    14581 ttctttcagc tcttcgattt ccatctgcaa cagagtgatg gtatcaatcg cctgctgtac
    14641 ttttgcttcc agtttctcaa acacttctaa tgacattgtc atacctctcc tgaattgcaa
    14701 ggcgttgatg gataaaaaat cctcgtcccg attaccggtg acgccttaat aaatacgagc
    14761 gcactttagt tagctccgat tgtatgaagc cgcgccatcg ctgtccagcg gcacgccttg
    14821 cagattacgg tttgccacac ttttcatcct tctcctggtg acataatcca catcaatcga
    14881 aaatgttaat aaatttgttg cgcgaatgat ctaacaaaca tgcatcatgt acaatcagat
    14941 ggaataaatg gcgcgataac gctcatttta tgacgaagca cacacatttt aagttcgata
    15001 tttctcgttt ttgctcgtta acgataagtt tacagcatgc ctacaagcat cgtggaggtc
    15061 cgtgactttc acgcatacaa caaacattaa ctcttcagga tccgattatg agtcaaacat
    15121 caaccttgaa aggccagtgc attgctgaat tcctcggtac cgggttgttg atttttttcg
    15181 gtgtgggttg cgttgcagca ctaaaagtcg ctggtgcgtc ttttggtcag tgggaaatca
    15241 gtgtcatttg gggactgggg gtggcaatgg ccatctacct gaccgcaggg gtttccggcg
    15301 cgcatcttaa ccccgctgtt accattgcat tgtggctgtt tgcctgtttc gacaagcgca
    15361 aagttatccc ttttatcgtt tcacaagttg ccggcgcttt ctgtgctgcg gctttagttt
    15421 acgggcttta ctacaattta tttttcgact tcgagcagac tcatcatatt gttcgcggca
    15481 gtgttgaaag tgttgatctg gctggcactt tctctactta ccctaatcct catatcaatt
    15541 ttgtgcaggc tttcgcagtt gagatggtga ttaccgctat tctgatgggg ctgatcctgg
    15601 cgttaacgga cgatggcaac ggtgtaccac gcggcccttt ggctcccttg ctgattggtc
    15661 tactgattgc ggtcattggc gcatctatgg gcccattgac gggttttgcc atgaacccag
    15721 cgcgtgactt cggtccgaaa gtctttgcct ggctggcggg ctggggcaat gtcgccttta
    15781 ccggcggcag agacattcct tacttcctgg tgccgctttt tggccctatc gttggcgcga
    15841 ttgtaggtgc atttgcctac cgcaaactga ttggtcgcca tttgccttgc gatatctgtg
    15901 ttgtggaaga aaaggagacc acaactcctt cagaacaaaa agcttcgctg taatatgact
    15961 acgggacaat taaacatgac tgaaaaaaaa tatatcgttg cgctcgacca gggcaccacc
    16021 agctcccgcg cggtcgtaat ggatcacgat gccaatatca ttagcgtgtc gcagcgcgaa
    16081 tttgagcaaa tctacccaaa accaggctgg gtagaacacg acccaatgga aatctgggcc
    16141 acccaaagct ccacgctggt agaagtgctg gcgaaagccg atatcagttc cgatcaaatt
    16201 gcagctatcg gtattacgaa ccagcgtgaa accactattg tctgggaaaa agaaaccggc
    16261 aagcctatct ataacgccat tgtctggcag tgccgtcgta ccgcagaaat ctgcgagcat
    16321 ttaaaacgtg acggtttaga agattatatc cgcagcaata ccggtctggt gattgacccg
    16381 tacttctctg gcaccaaagt gaagtggatc ctcgaccatg tggaaggctc tcgcgagcgt
    16441 gcgcgtcgtg gtgaattgct gtttggtacg gttgatacgt ggcttatctg gaaaatgact
    16501 cagggccgtg tccatgtgac cgattacacc aacgcctctc gtaccatgtt gttcaacatc
    16561 cataccctgg actgggacga caaaatgctg gaagtgctgg atattccgcg cgagatgctg
    16621 ccagaagtgc gtcgttcttc cgaagtgtac ggtcagacta acattggcgg caaaggcggc
    16681 acgcgtattc caatctccgg gatcgccggt gaccagcagg ccgcgctgtt tggtcagttg
    16741 tgcgtgaaag aagggatggc gaagaatacc tacggcactg gctgctttat gctgatgaac
    16801 accggcgaga aagcggtgaa atcagaaaac ggcctgctga ccaccatcgc ctgtggccca
    16861 actggcgaag taaactatgc gctggaaggt gcggtgttta tggcgggcgc atccattcag
    16921 tggctgcgcg atgagatgaa gttgattaac gacgcgtacg attccgaata tttcgccacc
    16981 aaagtgcaaa acaccaatgg tgtatatgtg gttccggcat ttaccgggct gggtgcgcca
    17041 tactgggatc cgtatgcgcg cggggcgatt ttcggcttaa cgcgtggagt gaacgctaac
    17101 cacattatcc gcgcgacgct ggagtctatt gcttatcaga cgcgtgacgt gctggaagcg
    17161 atgcaggccg actccggtat tcgtctgcac gcgctgcgcg tggatggcgg tgcagtagca
    17221 aacaatttcc tgatgcagtt ccagtccgat attctcggta cgcgcgttga gcgcccggaa
    17281 gtgcgcgaag tcaccgcttt gggtgcggcc tatcttgctg gtctggcggt tggcttctgg
    17341 cagaatcttg acgagctgca agagaaagcg gtgattgaac gcgagttccg tcctggcatc
    17401 gaaaccactg agcgtaatta ccgttacgca ggctggaaaa aagcggtcaa acgcgcgatg
    17461 gcatgggaag aacacgacga gtaataatga ttgcctgatg cgacgcttgc gcgtcttatc
    17521 aggcctacga gtcggataca catccggcat cacgcagact ctgaaaccgt gcgggggcaa
    17581 ccccgcacac atcaataatc ccgcccttcc cctgtgctac acttcgcgcc attccttact
    17641 gcttagagtt tgctatgaga cgagaacttg ccatcgaatt ttcccgcgtc accgaatcag
    17701 cggcgctggc tggctacaaa tggttaggac gcggcgataa aaacaccgcg gacggcgcgg
    17761 cggtaaacgc catgcgtatt atgctcaacc aggtcaacat tgacggcacc atcgtcattg
    17821 gtgaaggtga aatcgacgaa gcaccgatgc tctacattgg tgaaaaagtc ggtactggtc
    17881 gcggcgacgc ggtagatatt gctgttgatc cgattgaagg cacgcgcatg acggcgatgg
    17941 gccaggctaa cgcgctggcg gtgctggcag taggcgataa aggctgtttc ctcaacgcgc
    18001 cagatatgta tatggagaag ctgatcgtcg gaccgggagc caaaggcacc attgatctga
    18061 acctgccgct ggcggataac ctgcgcaatg ttgcggcggc gctcggcaaa ccgttgagcg
    18121 aactgacggt aacgattctg gcaaaaccgc gccacgatgc cgttatcgca gaaatgcaac
    18181 aactcggcgt acgcgtattt gctattccgg atggcgatgt ggcggcctca attctcacct
    18241 gtatgccgga cagtgaagtc gatgtactgt acggcattgg cggcgcgccg gaaggcgtgg
    18301 tttctgcggc ggtgatccgc gcattagatg gtgacatgaa cggtcgtctg ctggcgcgtc
    18361 atgacgtcaa aggcgacaac gaagagaatc gtcgcattgg cgagcaggag ctggcacgct
    18421 gcaaagcgat gggcatcgaa gccggtaaag tattgcgttt gggcgatatg gcgcgcagcg
    18481 acaacgtcat cttctctgcc accggtatta ccaaaggcga tctgctggaa ggcattagcc
    18541 gcaaaggcaa tatcgccacc accgaaacgc tgctgatccg tggtaaatca cgcaccattc
    18601 gtcgcattca gtccatccac tatctggatc gtaaagaccc ggaaatgcag gtgcacatcc
    18661 tctgattgat ttgatcgatt gagccttcca gtccttcggg actggaattt ttttgttcgg
    18721 agaacgaaga taaggcaagt caatcaaaac aggagaaaaa catggctgat tgggtaacag
    18781 gcaaagtcac taaagtgcag aactggaccg acgccctgtt tagtctcacc gttcacgctc
    18841 ccgtgcttcc gtttaccgcc gggcaattca ccaaacttgg ccttgaaatt gaaggcgaac
    18901 gcgttcagcg cgcctactcc tatgttaact cgcccgataa tccggatctg gagttttacc
    18961 tggtcaccgt ccccgatggc aaattaagtc cacgactggc ggcactgaaa ccgggcgatg
    19021 aagtgcaggt ggttagcgaa gccgctggct tctttgtgct ggatgaagta ccagattgcg
    19081 aaacgctatg gatgctggca accggtacag cgattggccc ttatttatcg attctgcaat
    19141 tgggcaaaga tttagaacgc ttcaaaaaca tggtgctggt tcacgccgca cgatatgccg
    19201 ccgacttaag ctatctacct ctgatgcagg aactggaaaa acgctacgag gggaaactgc
    19261 gcattcagac ggtggtcagt cgggaaacgg ctgcgggttc gctcactggg cgattaccgg
    19321 cgttaattga aagtggtgaa ctggaaagcg cggttggcct gccgatgaat aaagaaacca
    19381 gccatgtgat gctgtgcggc aatccacaga tggtgcgcga tacacaacag ttgctgaagg
    19441 agacccggca gatgacgaaa catttacgtc gccgaccggg ccatatgaca gcggagcatt
    19501 actggtaagc ggttacttgt cgataaacgg cacgatcaag agatccgcac tcaacttatt
    19561 gatcatcccg cgatatgccg gcatcaaacg gttgataaat gagtggtgat gaccacagac
    19621 aaggaggtcg cactgctctt tttgcataat ttccagcagt gtttccggca tttctccgcg
    19681 ttcaatacgc agttttgtct tcggccattg aatatttttc gtcagtttat acagcttgtt
    19741 atccgactta ttcttcaaca attgaagaat atcttctgtt gcagggaagt agatacccgg
    19801 atacaactcg cttaagccat catcaatatg aattaacgtc aggtgagcgt cattatgtct
    19861 ggcgagctcc agggctttat tcaccagtaa ggcatcttct tcattcccgg aaattgccac
    19921 gccaatgtgt ttataagcca tatttaactc cttctaaagc aactccatca agctaacgaa
    19981 cgcagtgata actcaaaaat aatcatctcg gcctggcatg agaaggtgaa ggccgcatca
    20041 agctcaactt gcccttccag atccttaatc ttataggcca cgctggcccc ttcatctgca
    20101 gaggatgcag cggcggcaag ctctttcagt ttggtcataa aggactctgc ttcttcgcgg
    20161 gtggcaaaaa agcgcgaaaa cttactggta cagttgtcgt tatcaatcac cgtaccgata
    20221 tcaatcgcac atcctttagt actgcattta tctacgacat ctttcatagg gcacctctgt
    20281 atattcgcct ctctgtcgat tacgacttaa gaataatgcg ccgttaaagg aagaacaaac
    20341 aagtaatgat cttatggatt accatttttt ttgatattgc ttaaggtgaa gagttcagaa
    20401 ggattttccg ggcattgtca cttgcatcgt ggaaataaca atcccggaaa aaaatagcga
    20461 taagagatat attaaaggtt aattacttga tttattgtcg gctttatact tcacatcctg
    20521 ggtatcttta ccatatttat tttcgccttg tgtgccaaca aacgcaccaa gatcgataag
    20581 catcatcacc agaatcaatg tcgggacaaa acgccccacc gcccattgcc agacacccgg
    20641 taaaattgcc cagttacccg ccagcagcat ccacgccaca atcatcagaa atgcccatgc
    20701 gccggaacgc ccgcgatcat gcaagcgctt aacggttact gccgctgttg gccaaagcaa
    20761 gcacacaagg caaaacgccg cggtctgaat atcgagtaaa tttttacccg ccagtgagaa
    20821 aagcaccagc atgcctgcga accacaggcc tatccaaatc cagaaatcac ggcgtccaat
    20881 acgcccttta aatgagaata accattgctg tatggtcatg taagttcctt gatggttgtc
    20941 ttttccagga ttctaccctt ttgacaaggg cgacagatat cgttttaatc ggagccagtc
    21001 ataacaaaag gtactgtcaa tgaagccagg gtgtacgctg ttttttctct tatgttctgc
    21061 attaaccgtt acaacaacgg cgcatgcaca aacaccagat acggcaacga ccgcgcctta
    21121 tctgctggct ggagccccta ctttcgatct ctccatcagc cagtttcgag aagactttaa
    21181 cagccagaat cccagcctgc cactgaacga atttcgtgcc atcgacagca gtcccgacaa
    21241 agccaatctc actcgtgcag caagtaaaat taacgagaac ttgtatgctt ctactgcgct
    21301 ggagcgcggt accttaaaaa tcaaaagcat tcaaatgacc tggctaccca tccaggggcc
    21361 agagcaaaaa gccgcgaaag cgaaagctca ggcatacatg gcagcggtga tccgcacact
    21421 caccccatta atgaccaaaa ctcaaagcca gaaaaaactg caggcgctac taacggcggg
    21481 gaaaaacaaa cgttattaca ccgagacaga aggtgcactg cgttatgttg tcgcggacaa
    21541 cggcgaaaag gggctgacct tcgctgttga accgattaag ctggcgctat ctgaatcgct
    21601 tgaaggtttg aataaatgac aaaaagcaaa gcctttgtgc cgatgaatct ctatactgtt
    21661 tcacagacct gctgccctgc ggggcggcca tcttccttta ttcgcttata agcgtggaga
    21721 attaaaatgc gacatccttt agtgatgggt aactggaaac tgaacggcag ccgccacatg
    21781 gttcacgagc tggtatctaa cctgcgtaaa gagctggcag gtgttgctgg ctgtgcggtt
    21841 gcaatcgcac caccggaaat gtacatcgat atggcgaagc gtgaagctga aggcagccac
    21901 atcatgctgg gtgcgcaaaa cgtggacctg aacctgtccg gcgcattcac cggtgaaacc
    21961 tctgctgcta tgctgaaaga catcggcgca cagtacatca tcatcggcca ctctgaacgt
    22021 cgtacttacc acaaagagtc tgacgaactg atcgcgaaaa aattcgcggt gctgaaagag
    22081 cagggtctga ctccggttct gtgcatcggt gaaaccgaag ctgaaaacga agcgggcaaa
    22141 actgaagaag tttgtgcacg tcagatcgac gcggtactga aaactcaggg tgctgcggca
    22201 ttcgaaggtg cggttatcgc ttacgaaccc gtatgggcaa tcggtactgg caaatctgca
    22261 actccggctc aggcacaggc tgttcacaaa ttcatccgtg accacatcgc taaagttgac
    22321 gctaacatcg ctgaacaagt gatcattcag tacggcggct ctgtaaacgc gtctaacgct
    22381 gcagaactgt ttgctcaacc agacatcgac ggcgcgctgg ttggcggtgc ttctctgaaa
    22441 gctgacgctt tcgcagtgat cgttaaagct gcagaagcgg ctaaacaggc ttaagtctga
    22501 caggtgccgg atttcatatc cggcacttac tttccttaac tcctcgcctt aacgcaaaat
    22561 ctcacactga tgatcctgga tttcctcggc tgaagcacgg ttaagcgtca gtagatttcg
    22621 ttgtgtcgcc agcaatacaa atgagttatc actctgccgc accatcgcca gcccgtagct
    22681 tcccatatgt tcccgcgcct caggcacttc ttctgcaagc atcataaatg ggctgcgttg
    22741 taccagttcg ctttccgtta cccgacgcgc caggtattca tgcccgcgca aaccacctgg
    22801 cagtggcaac cagcggctgc tgatgttcgc cagattgtta tccagctgtt cgcgcacatc
    22861 aggacgaata caagagatat gaatatgaaa atggttttgc gtacgcccgg tgcgagagtt
    22921 gatcgccaaa gaaaccgcgc gatcgggaac cggctggccg tattttttgc tcataaaatc
    22981 acgcgcctgc caggccaacc aaaagaagtt cggcgttgaa ggatcggtca acaaaggact
    23041 ttcagtaccg ttaatacgat acgttggcat caacagatat tgcagtgggc cattaagatc
    23101 ttttaaaacc acgtatccgg cattgggttt gacttccgca catggcgaag gattttgatt
    23161 ttgctgctga ttgggcaaac attcctcaag gacaatctta cgtaatgtat ccgactcttc
    23221 accggttaat ttccagtaac caataccggc agccacaacg gcgataacta tcatcaccaa
    23281 aaaaagaaga cccgcttttt tcatcttttt ttccctgtac ctcaaagaga tgcaagggta
    23341 acgcaaaatc gtgacaaata aaaaacccgg tcgcgagaaa ccaccgggtt tgataattat
    23401 cctggagaaa tcagcgtttg ctaatctgat cgaacgtacc gccgttagca aaatgctctt
    23461 tttgcgcttt cgtccagccg ccgaactctt catcaatggt gaataacttc agctttggaa
    23521 acgcattttc gtactttttc gccacctcag catcgcgcgg acggtagtag tttttcgcgg
    23581 caatttcctg accttctggc gagtagagat atttcaggta ggcttccgcc acctctttgg
    23641 tgcctttttt ctcgaccact ttatcgacca ccgacacggt tggctctgcg aggatagact
    23701 cactcggcgt gacgatttcg aatttatctt tccccagctc attcgctgcc agcagagctt
    23761 cgttttccca ggcgatcagt acatcgccaa tcccgcgctc gacaaaggta ttagtggagc
    23821 cgcgcgcgcc agaatccaga acttcgacgt ttttatacag tgcccgaaca aaatcctgtg
    23881 cttttgcctg atcgttattg ttgtgatgca gcgcgtagcc ccaggctgcc aggtagttcc
    23941 agcgcgcgcc accagagctt ttcggattag gcgtgatcac cgaaacaccc ggtttaatca
    24001 gatcgttcca gtcatggatc tgcttcggat tgcccttacg caccaggaaa acaatggtgg
    24061 aagtgtacgg tgcggagtta tccggcagac gtttgatcca ctctttatca atccgcccgc
    24121 gttccgcaat cgcgtccacg tcataggcca gagccagcgt gacaacatca gcttcaatac
    24181 cgttgattac cgacgtcgct tgtttgcctg aaccaccgtg ggactgacgg atcaccacgt
    24241 tatcgccagt ttgctgtttc cagtgggcgc tgaatacctt gttgtactgt tcgtacaatt
    24301 cgcgcgttgg atcatatgaa acgttaagaa gctgaatatc ctttgccata acgctggttg
    24361 ccgccagcaa aaatgttaac cctacgcccc acttgttcat cgcccgactc tcttatgttg
    24421 tgttgtgatg agcaaagcgt gccagaaggt taaccaaaca ttaaagaata aaaaaagatt
    24481 ggctataact tgcgggtata tgttggggga ttaaaaaggc ggaagaaatc cgcctcatat
    24541 tgctgacaaa gtgcgctttg tccatgccgg atgcggcgcg aacgccttat ccggcctaca
    24601 aaagttcgca aattcaataa attgcagaat tcatgtaggc ctgataagcg aagcgcatca
    24661 ggcatttttg cttctgtcat cggtttcagg ctaaaggaat ctgccttttt ccgaaatcat
    24721 taatacagtt ttttcgcgca gtccagccag tcgcctttga acggacgctt catgttttcg
    24781 atagcgtcga tgatgtcgtg gtgaaccagc tgttcgttct ggatacctac gcaacgacca
    24841 ccgtaacctg ccagcagcag ctcgatagcg taagcgccca tacgggaagc cagaatacgg
    24901 tcgtaaggca ccggagaacc accgcgctgg atgtggccca gcacagttgc gcgggtttca
    24961 cgaccggttt ctttctcgat gaaatgcgcc agttcgtcaa catcacacat atgttcggta
    25021 atcgccacta tcgcgtgttt tttacctttc gcgatacccg ctttgatttc gtttaccagg
    25081 tcttcacggc tgaattcaac ttccggaacc acaacgaatt cacagccacc ggcaatagcc
    25141 gcagccaacg tcagatcgcc acaataacgg cccatcactt ccaccacgga aatacgctgg
    25201 tgagaagaag aggtgtcacg cagacggtcg atcgcttcta caacggtgct cagcgcagtg
    25261 aagaaaccga tggtgtagtc agtgcctttg atgtcgttgt cgatagtgcc cggcagaccg
    25321 atgcacggga agcccatttc ggtcagacgc attgcaccca tgtaggaacc gtcaccgccg
    25381 ataaccacca gcgcgtcgat accacgtttt ttcaggtttt cgatagccac ggcgcggatg
    25441 ttctcgtcgc ggaattccgg gaaacgcgca gaaccgagga acgtaccacc acggttgatc
    25501 atgtcagaaa cgctgtaacg gtctagctgt accatacggt cttcatacag acccagatag
    25561 ccgtcataaa tgcccattac ttccagacct tctgtcagcg cagaacgaac aaccccgcga
    25621 attgcggcgt tcatgcctgg cgcatcaccg ccgcttgtca acacaccgat tttcttaatc
    25681 atgactacct ctgaactttg gaatgcaaaa tgaaatctgt tgccggaagt cttcttgcac
    25741 atcgaagtga tccaacgaat gtgcaaatag tataacaatc acttcctgct gaattgattc
    25801 aggtcaggcc aaatggcggt attttataca caaaatgcgg gtctggctct cttttatact
    25861 gattatgaaa gcatagaccg tttaccctcc ctgggtacga cggaacaggg gtcctgatgg
    25921 ataatcacat ccgatcccgg aaaacgccgt aaaatagcct gctctacctg atccgccacc
    25981 atatgtgcct gaaccaaagg cagagagtct tccatttcca aatgaatctg aataaagcgg
    26041 gtcggccctg actgccgcgt gcgaagatcg tgagcgccgc taacacccgg ccaggaagtc
    26101 acgatatcaa taatttcttg ccgttcttca tcaggcaatg cgcgatccag taatgactgt
    26161 accgcctcat atcccatgcg taacgcgcta tataaaatat agatgccgat tcccaatgca
    26221 aacagagcat cggcgcgatg ccagccgtac caggacaacc ccagcgccag cagaattgca
    26281 ccgttcatca taacatcaga ctggtaatgt agcatatcag cccgcaccgc ctggctttgc
    26341 gtccggcgca ccacccaacg ctgaaacgag acaaggataa tcgtacaaat tagcgccaca
    26401 attgtcacga taaccccgac gcctggatct gtcatcggtg ttggagatat cagatgttga
    26461 atacccgtca aaaacaggaa tagtgctgaa ccggagataa acatactttg cgccagcgcc
    26521 gcgagggact cagctttacc gtgaccaaac gagtgattat cgtcggcagg ttgcagggaa
    26581 tatcgcacca ccagtaaatt cgtcaacgac gcgccgatat ccaccagcga atccaccagc
    26641 gcggcgagaa tactcaccga cccggtatac caccatgcaa aaattttaat cagcaatagc
    26701 agtgaagcca tcgccgttgc agcaatcgcc gcccgactga ccagccgtcc ataagattga
    26761 ttcataaata ctcccgctat caactgacgg tagtataacg gaagcaaatc atctgcaatg
    26821 cattaagcag taggcaaatt gaggataaaa aaaaccccca catcatgtgg gggaagacag
    26881 ggatggtgtc tatggcaagg aaaacagggt ttactactgg gaacgtgagt tgctactact
    26941 caatagcttc aacgatgaac ttttttgcca ttgcgtcacg tcgcgcaact gctccattcg
    27001 ttgttgatgt ttctcgttta aaaccgcttg ctgctccggc gttaacaggc gatacatttg
    27061 gttgcggact tttgccatct caacctgacg agcaatttgc tcattcgcca ttttttctgc
    27121 ctgcgcgcgc acagcgtttt catcaaaatt ttctgcggtg acaaggcgat gcattgtctc
    27181 cagttcgcta acattaacag gaggctgttc gtgccgggcc tgttgcataa gatctcgcat
    27241 ctgctgacgc tgatgttcgg ttaaacttat gccgtcgaac atatggctct gcgtactgcg
    27301 ctgcgtaagt tcttcacccg gatgccagtt atcgcctgaa ccgacttcag cagcgtggct
    27361 taatgaactg actgccagcg ttgaggccat gacggcagcg gtaactatgc gcatcatttg
    27421 ctcccaaaat ctttctgtcg cgattcaacg atagagagtt tacgattcag gctgcaaaca
    27481 tgcgtcaggg ggtgtaaaac aacgtaaagt catggattag cgacgtctga tgacgtaatt
    27541 tctgcctcgg aggtatttaa acaatgaata aaatcctgtt agttgatgat gaccgagagc
    27601 tgacttccct attaaaggag ctgctcgaga tggaaggctt caacgtgatt gttgcccacg
    27661 atggggaaca ggcgcttgat cttctggacg acagcattga tttacttttg cttgatgtaa
    27721 tgatgccgaa gaaaaatggt atcgacacat taaaagcact tcgccagaca caccagacgc
    27781 ctgtcattat gttgacggcg cgcggcagtg aacttgatcg cgttctcggc cttgagctgg
    27841 gcgcagatga ctatctcccg aaaccgttta atgatcgtga gctggtggca cgtattcgcg
    27901 cgatcctgcg ccgttcgcac tggagcgaac aacagcaaaa caacgacaac ggttcaccga
    27961 cactggaagt tgatgcctta agtctcaatc ctggtcgcca ggaagccacc ttcgacgggc
    28021 aaacactgga gttaaccggt actgagttta ccctgctcta tttgctggca cagcatctgg
    28081 gtcaggtggt ttcccgtgag catttaagcc aggaagtgct gggcaagcgc ctgacgcctt
    28141 tcgaccgcgc tatcgatatg catatttcca acctgcgtcg taaactgccg gatcgtaagg
    28201 atggtcaccc gtggtttaaa accctgcgtg gtcgtggcta tctgatggtt tctgcttcat
    28261 gataggcagc ttaaccgcgc gcatcttcgc catcttctgg ctgacgctgg cgctggtgtt
    28321 gatgttggtt ttgatgttac ccaagctcga ttcacgccag atgaccgagc ttctggatag
    28381 cgaacagcgt cagggtctga tgattgagca gcatgttgaa gcggagctgg cgaacgatcc
    28441 gcccaacgat ttaatgtggt ggcggcgtct gttccgggcg attgataagt gggcaccgcc
    28501 aggacagcgt ttgttattgg tgaccaccga aggccgcgtg atcggcgctg aacgcagcga
    28561 aatgcagatc attcgtaact ttattggtca ggccgataac gccgatcatc cgcagaagaa
    28621 aaagtatggc cgcgtggaac tggtcggtcc gttctccgtg cgtgatggcg aagataatta
    28681 ccaactttat ctgattcgtc cggccagcag ttctcaatcc gatttcatca acttactgtt
    28741 tgaccgcccg ctattactgc tgattgtcac catgctggtc agtacgccgc tgctgttgtg
    28801 gttggcctgg agtctggcaa aaccggcgcg taagctgaaa aacgctgccg atgaagttgc
    28861 ccagggaaac ttacgccagc acccggaact ggaagcgggg ccacaggaat tccttgccgc
    28921 cggtgccagt tttaaccaga tggtcaccgc gctggagcgc atgatgacct ctcagcagcg
    28981 tctactttct gatatctctc acgaactgcg caccccgctg acgcgtctgc aactgggtac
    29041 ggcgttactg cgccgtcgta gcggtgaaag caaggaactg gagcgtattg aaaccgaagc
    29101 gcaacgtctg gacagcatga tcaacgatct gttggtgatg tcacgtaatc agcagaaaaa
    29161 tgcgctggtt agcgagacca tcaaagccaa ccagttgtgg agtgaagtgc tggataacgc
    29221 ggcgttcgaa gccgagcaaa tgggcaagtc gttgacggtt aacttcccac ctgggccgtg
    29281 gccgctgtac ggcaacccga acgccctgga aagtgcgctg gaaaacattg ttcgtaatgc
    29341 tctgcgttat tcccatacga agattgaagt gggctttgcg gtagataaag acggtatcac
    29401 cattacggtg gacgacgatg gtcctggcgt tagcccggaa gatcgcgaac agattttccg
    29461 tccattctat cggaccgatg aagcacgcga tcgtgaatct ggcggtacag gtttggggct
    29521 ggcgattgta gaaaccgcca ttcagcagca tcgtggctgg gtgaaggcag aagacagccc
    29581 gctgggcggt ttacggctgg tgatttggtt gccgctgtat aagcggagtt aaactccgca
    29641 tttgtaggca ggataaggcg tttacgccgc atccggcatt tgagcaggat gcctgatgcg
    29701 acgctgatag cgtcttatca ggcctacact ccgggggtta ttttccccac aatcggcggg
    29761 aaaaatcttc gatcttgccg ctaagtcggc gcttttgcat cgttcgcgtc cagcttttcg
    29821 ataaccctgc ggcggagagt aaacggtgat actgatcgtc atcaaacggc atatgccatg
    29881 cgatggcggc agcctcctgc acggtcacat cgctgacacg ggaaaccaac tccagcggcg
    29941 catcagcaga taccaaccct ggtgcaatta cgctatacaa ccaccccact ttacccgtgt
    30001 tttgcatcaa ctgcgctata tcgctgatat caaaatggta attgagttta tagcaaggtg
    30061 agcgtggctg gctgacctga attaatgcct ctccccagcg gaaaatatca ccaatataaa
    30121 cattactttc cgtcagaccg tcggttgaga gattttcgcc aaacgcaggc gcaacaaaca
    30181 gctccgcctg ttccggaaat tcccgcgccc agtagagata atgctcacga ggataatgac
    30241 acagcgctct gtctgacccg ccgtggatct ttttctccgc ctgttcgtca ccttccagcc
    30301 ccaggtccgt cagcatcaac tcaccatcaa cctggatttt ggcaattgcg ctgggtttgc
    30361 cttcgggata agcctgaatc ttgcctgtgt atacatcaac cggatatcgc atctgttccc
    30421 tctccacgag aaatttcccc attagaccat atttatttta tgtcaggcgt gctgttttcg
    30481 cggtaagtat ccaggcagaa acgcaattga aaaccggacg aacgttagtt catccggctg
    30541 tggggattaa ggaagttggt ttatttcacc gtacctaaaa tttcgatttt cgccgctctg
    30601 gctttgactt tacgtgacca gatagaggta agaattggca ccagaatcga agtcacaatg
    30661 accgccgtcg cgaccagcga agttgctgcc ggagccatag gtttaaacgc aggcaccatt
    30721 tctgcaatca gcacaggtgt cgctaccgcg gcccctgcgg aactggaagc ggcaattccg
    30781 gcagtgccat cgccaccgcc aatcaattta tcggcgataa tcaacggaat accggtcacg
    30841 ataattactg ccacacccaa caggatcccc agcaaacctg tctgggcaat tacagtcaaa
    30901 tcaatggtat tgcccagtgc gaaggcaaag aatggaataa gcgtctgcac cgctttgctg
    30961 aaaaattctc gtaactcagg gtcaaggttc cccagggcaa agcccaccag gaacggcaat
    31021 actgcgccga cgaaaacatg cggttcaaac gaggcaatcc cggcagtgcc cagaataatc
    31081 atcgtcatga gcggcccgga ctccaacgac atcaacacaa atgccccagc ttcttctttt
    31141 gtgccgtact gctgcatgat ggaagcgtaa agtccgccgt tggtcatatc catcgccgcc
    31201 accagcgcca gcgttgaaag tccggcaaag aatccaactt caacaccatg ttccggaata
    31261 atgcgcgagg caattgccgc aaccacccac gcgacggcaa tttttgttac caccagcgta
    31321 ccggatttac gcaataccgt tcccgtcgcg cttaatttta ttgacgcccc catgcaaaaa
    31381 aaccacaccg ccagaatggg caccgtaccg gtaatcatcc cgttggtgaa tgatccaaaa
    31441 tatttccccg cccccggcga gaaggtgtgg cacagtgcgc caaggaatag cgggacgagc
    31501 atcatccccc ccgggatttt ctcaatcgag cgttttatct gcatttccat cacctgtcgg
    31561 catattttta agggctgatg cgtcattcct caacggagca tcagtgttga tggaaacgaa
    31621 gatagacatt catttgatca ataaaagtga tcaaaaccac atcaattgaa acgctgtttt
    31681 atttttatcg gatcattgtt tttattttga aattgatcac aaaaaaacca ccgccgttgg
    31741 cgatggttca ttttttaagc tgcaatgcgg cgtaaacgcc tcattgcagc aggcatcaaa
    31801 tgattatttt ttcgccgcga aacgtgccgc tgcttcgtcc cagttcacca cgttccagaa
    31861 ctctttaatg tagtccggac ggcggttctg gaatttcagg tagtaagcat gttcccacac
    31921 atccaggccc acaatcggga agccggaagc gccagaaata gcttcaccca tcagcgggga
    31981 gtcctggtta gcagtagaaa ccaccgccag tttatcgcct ttcagcacca gccatgccca
    32041 gccggaacca aagcgggaag ctgccgcttt ttcaaattct gctttgaaat tatcaacgga
    32101 accgaaatca cgttcgatag ccgctttcag gtcgccctgc agggtggtgc cttttttcag
    32161 acctttccag aacagactgt ggttagcgtg accaccagcg ttattgcgca gtacggtttt
    32221 cttgtctgct ggcagctggt ccagtttggt gatcagctct tcaaccggca ggttggcaaa
    32281 ttctggcagg ttttccagcg ccgcgttggc gttgttgacg taagtctgat ggtgtttggt
    32341 gtggtggatt tccatggtct gcttatcgaa gtgcggttcc agggcatcgt aagcatacgg
    32401 cagggatggc agggtataac tcatattcat ctccagtgtt gtcgggcggc cgattgttaa
    32461 tgccgcgtaa gcagttggtt cattatagtt aattaaatga tattgaaaat gattatcaat
    32521 gccgtacttt tcataagggt atggttttgc aggaaaatgc ccaagatgtg aagcaaatca
    32581 cccacttaat gccgtgattg ccagtaaatc ggcagcggcg gcaacaggcg aaaggttaat
    32641 cgacagctca atttttacac tcatctcgtc ggagatgtga cgcgacgaaa aaatgatgag
    32701 gataagaaga tgagtaacgc gattacgatg gggatatttt ggcatttgat cggcgcggcc
    32761 agtgcagcct gtttttacgc tccgttcaaa aaagtaaaaa aatggtcatg ggaaaccatg
    32821 tggtcagtcg gtgggattgt ttcgtggatt atcctgccgt gggccatcag cgccctgtta
    32881 ctaccgaatt tctgggcgta ttacagctcg tttagtctct ctacgctgct gcctgttttt
    32941 ctgttcggcg ctatgtgggg gatcggtaat atcaactacg gcctgaccat gcgttatctc
    33001 ggcatgtcga tgggaattgg catcgccatt ggcattacgt tgattgtcgg tacgctgatg
    33061 acgccaatta tcaacggcaa tttcgatgtg ctgattaata ccgaaggcgg acgcatgacg
    33121 ttgctcggcg ttctggtggc gctgattggc gtagggattg taactcgcgc cgggcagttg
    33181 aaagagcgca agatgggcat taaagccgaa gagttcaacc tgaaaaaagg gctggtgctg
    33241 gcggtgatgt gcggcatttt ctctgccggg atgtcctttg cgatgaacgc cgcaaaaccg
    33301 atgcatgaag ccgctgctgc actgggcgtc gatccactgt atgtcgctct gccaagctat
    33361 gttgtcatca tgggcggcgg cgcgatcatt aacctcggtt tctgcttcat tcgtctggca
    33421 aaagtgaagg atttgtcgct aaaagccgac ttctcgctgg caaaaccgct gatcattcac
    33481 aatgtgttac tctcagcact gggcgggttg atgtggtatc tgcaattctt tttctatgcc
    33541 tggggccacg cgcgtattcc ggcgcagtat gactacatca gttggatgct gcatatgagc
    33601 ttctatgtat tgtgcggcgg tatcgtcggg ttggtgctga aagagtggaa caatgcaggt
    33661 cgccgtccgg taacggtgtt gagcctcggt tgtgtggtga ttattgtcgc cgccaacatc
    33721 gtcggcatgg gcatggcgaa ttaatctttc tgcgaattga gatgacgcca ctggctgggc
    33781 gtcatcccgg tttcccgggt aaacactacc gaaaaatagt tactatcttc aaagccacat
    33841 tcggttgaaa tatcactgat taacaggcgg ctatgctgga gaagatattg cgcatgacac
    33901 actctgacct gccgcagata ttgattgatg gtcattccag tctgctggcg aaattgctga
    33961 cgcaaaacgc gctcactgca cgatgcctca tcacaaaatt tatccagcgc aaagggactt
    34021 ttcaggctgg ccgccagccg ggtaatcagc ttatccagca acgtttcgct ggatgttggc
    34081 ggcaacgaat cactggtgta acgatggcga ttcagcaaca tcaccaactg cccgaacagc
    34141 aactctgcca tttcgttagc aaacggcaca tgctgactac tttcatgctc aagctgaccg
    34201 ataatctgcc gcgcctgcgc catccccacg ctacctaagc gccagtgtgg ttgccctgcg
    34261 ctggcactaa atcccggaat cgccccctgc cagtcaagat tcagcttcag ccgctccggg
    34321 caataaataa tattctgcaa aaccagatcg ttaacggaag cgtaggagtg tttatcgtca
    34381 gcatgaatgt aaaagagatc gccacgggta atgcgataag ggcgatcgtt gagtacatgc
    34441 agaccattac cgcgccagac aattaccagc tcacaaaaat catgtgtatg ttcagcaaag
    34501 acatcttgcg gataacggtc agccacagcg actgcctgct ggtcgctggc aaaaaaatca
    34561 tctttgagaa gttttaactg atgcgccacc gtggctacct cggccagaga acgaagttga
    34621 ttattcgcaa tatggcgtac aaatacgttg agtagattcg cgttattgca gaaagccatc
    34681 ccgtccctgg cgaatatcac gcggtgacca gttaaactct cggcgaaaaa gcgtcgaaaa
    34741 gtggttactg tcgctgaatc cgcagcgata ggcgatgtca gtaacgctgg cctcgctgtg
    34801 gcgtagcaga tgtcgggctt tcatcagtcg caggcggttc aggtaacgct gaggcgtcag
    34861 tcccgtttgc tgcttaagct gccgatgtag cgtacgcagc gaaagagaaa atagatccgc
    34921 cacggcatcc caattcacct catcggcaaa atggtcctcc agccaggcca gaagcaagtt
    34981 gagacgtgac gcgctgtttt ccaggttctc ctgcaaactg cttttacgca gcaagagcag
    35041 taattgcata aataagatct cgcgactggc ggtcgagggt aaatcatttt cctcttcctg
    35101 ctgttccatc tgtgcaacca gctgtcgcac ctgctgcaac acgctgtggt taacgcgcca
    35161 gtgagacgga tactgcccat cctgctcttg tggcagcaac tgattcagcc cggcgagaaa
    35221 ctgaaatcga tccggcgagc gatacagcac attggtcaga cacagattat cggtatgttc
    35281 atacagatgc cgatcatgat cgcgtacgaa acagaccgtg ccaccggtga tggtataggg
    35341 ctgcccatta aacacatgaa tacccgtgcc atgttcgaca atcacaattt catgaaaatc
    35401 atgatgatgt tcaggaaaat ccgcctgcgg gagccggggt tctatcgcca cggacgcgtt
    35461 accagacgga aaaaaatcca cactatgtaa tacggtcata ctggcctcct gatgtcgtca
    35521 acacggcgaa atagtaatca cgaggtcagg ttcttacctt aaattttcga cggaaaacca
    35581 cgtaaaaaac gtcgattttt caagatacag cgtgaatttt caggaaatgc ggtgagcatc
    35641 acaccaccac aattcagcga attgtgaaca tcatcacgtt catctttccc tggttgccaa
    35701 tgacccattt tcctgtcagt aacgagaagg tcgcgaattc aggcgctttt tagactggtc
    35761 gtaatgaaat tcagcaggat cacattatga cctttcgcaa ttgtgtcgcc gtcgatctcg
    35821 gcgcatccag tgggcgcgtg atgctggcgc gttacgagcg tgaatgccgc agcctgacgc
    35881 tgcgcgaaat ccatcgtttt aaaaatgggc tgcatagcca gaacggttat gtcacctggg
    35941 atgtggatag cctggaaagt gccattcgcc ttgggctaaa caaggtgtgc gaggaaggga
    36001 ttcgtatcga tagcattggg attgatacct ggggcgtgga ctttgtgctg ctcgaccaac
    36061 agggtcagcg cgtgggcctg cccgttgctt atcgcgatag ccgcaccaat ggcctgatgg
    36121 cgcaggcaca acaacaactc ggcaaacgca atatttatca acgtagcggc atccagtttc
    36181 tgcccttcaa tacgctttat cagttgcatg cactgacaga gcaacaacct gaacttattc
    36241 cacacattgc tcacgctctg ctgatggcgg attactttag ttatcgcctg accggcgaga
    36301 tgaactggga gtacaccaac gccacgacca cgcaactggt caatatcaat agcgacgact
    36361 gggacgagtc gctactggcg tggagcggtg ccaacaaagc ctggtttggt cgcccgacgc
    36421 atccgggtaa tgtcataggt cactggattt gcccgcaggg taatgagatt ccggtggtcg
    36481 ccgttgccag ccatgatacc gccagcgcgg ttatcgcctc gccgttaaac ggttcacacg
    36541 ccgcttatct ctcttcgggc acctggtcat tgatgggctt cgaaagccag acgccattta
    36601 ccaatgacac ggcgctggcc gccaatatca ccaatgaagg tggggcggaa ggtcgctatc
    36661 gggtgctgaa aaatattatg ggcttatggc tgcttcagcg ggtgcttcag gagcggcaaa
    36721 tcaacgatct cccggcgctt atcgccgcga cacaggcact gccggcttgc cgcttcatca
    36781 tcaatcccaa tgacgatcgc tttattaatc ctgacgagat gtgcagcgaa attcaggctg
    36841 cgtgtcggga aacggcgcaa ccgatcccgg aaagtgatgc tgaactggcg cgctgtattt
    36901 tcgacagtct ggcgctgctg tatgccgatg tgttgcatga actggcgcag ctacgcggcg
    36961 aagatttctc gcaactgcat attgtcggcg gcggctgcca gaacgcgctg ctcaaccagt
    37021 tatgtgccga tgcctgcggt attcgggtga tcgccgggcc agttgaagcc tcgacgctcg
    37081 gcaatatcgg catccagtta atgacgctgg atgaactcaa caatgtggat gatttccgtc
    37141 aggtcgtcag caccaccgcg aatctgacca cctttacccc taatcctgac agtgaaattg
    37201 cccactatgt ggcgcagatt cactctacac gacagacaaa ggagctttgc gcatgaccac
    37261 tcaactggaa caggcctggg agctggcgaa acagcgtttc gcagcggtag ggattgatgt
    37321 cgaggaggcg ctgcgccaac ttgatcgttt acccgtgtca atgcattgct ggcagggcga
    37381 tgatgtttcc ggttttgaaa acccggaagg ttcgctgacc ggggggattc aggctactgg
    37441 caattatccg ggcaaagcgc gtaatgccag cgagctacgt gccgatctgg aacaagctat
    37501 gcggctgatt ccgggtccga aacggcttaa tttacatgcc atctatctgg aatccgatac
    37561 gccagtctcg cgcgaccaaa tcaaaccaga gcacttcaaa aactgggttg aatgggcgaa
    37621 atccaatcag ctcggtctgg attttaaccc ctcctgtttt tcgcatccgc taagcgccga
    37681 tggctttacg ctttcccatg ccgacgacag cattcgccag ttctggattg atcactgcaa
    37741 ggccagccgc cgcgtttcgg cctattttgg cgagcaactt ggcacgccat cggtgatgaa
    37801 catctggatc ccggatggca tgaaagatat caccgttgac cgtctcgctc cgcgccagcg
    37861 tttgctggca gcactggatg aggtgatcag cgagaagctg gatccggcgc accatatcga
    37921 cgccgttgag agcaaattgt ttggcattgg cgcggagagc tacacggttg gctccaatga
    37981 gttttacctg gggtatgcca ccagccgcca gacggcgctg tgcctggacg ccgggcattt
    38041 ccacccgact gaagtgattt ccgacaagat ttccgccgcc atgctgtatg tgccgcagtt
    38101 gctgctgcac gtcagccgtc cggttcgctg ggacagcgat cacgtagtgc tgctggatga
    38161 tgaaacccag gcgattgcca gtgagattgt tcgtcacgat ctgttcgacc gggtgcatat
    38221 cggcctcgac ttcttcgatg cctctatcaa ccgcattgcc gcgtgggtca ttggtacacg
    38281 caatatgaaa aaagccctgc tacgtgcgtt actggaacct accgctgaac tacgcaagct
    38341 ggaatcggcg ggcgattaca ctgcgcgtct ggcgctactg gaagagcaga aatcgttgcc
    38401 gtggcaggcg gtatgggaaa tgtattgtca gcgtcacgat acgccagcgg gtagcgaatg
    38461 gctggagaac gtgcgggctt atgagaaaga aattttgagt cgccgcgggt aaacactgcc
    38521 tgatgcggcg caagcgcctt atccggccta cgggtcggca atagttgtag gcctgataag
    38581 acgcgacagc gtcgcatcag gcattgattg ccggatgcgg cacgagtgcc ttatccggcc
    38641 tacagatcgt atatccggca acaggcacgg acgtccaatt aacaggaaca cagaacatgc
    38701 aaaacataac ccagtcctgg tttgtccagg gaatgatcaa agccaccacc gatgcctggc
    38761 tgaaaggctg ggacgagcgc aacggcggca acctgacgct gcgcctggat gacgccgata
    38821 tcgcaccata tcacgacaat tttcaccaac aaccgcgcta tatcccgctc agccagccca
    38881 tgcctttact ggcaaataca ccgtttattg tcaccggctc gggcaaattc ttccgtaacg
    38941 tccagcttga tcctgcggct aacttaggcg tcgtaaaagt cgacagcgac ggcgcgggct
    39001 accacattct ctgggggcta actcacgaag ccgttccaac cactgaactc ccggcgcact
    39061 tcctctccca ctgcgaacgc attaaagcca ccaacggtaa agatcgggtg atcatgcact
    39121 gccatgccac caacctgatc gcccttacct acgtactgga aaacgacacc gcggtcttca
    39181 ctcgccaact gtgggaaggc agcaccgaat gtctggtagt gttcccggat ggcgtcggca
    39241 ttttgccgtg gatggtgccc ggcaccgacg aaatcggcca ggcgaccgca caggaaatgc
    39301 aaaaacattc gctggtgttg tggcccttcc acggcgtctt cggcagcggt ccgacactgg
    39361 atgaaacatt cggtttaatc gacaccgcag aaaaatcagc acaagtatta gtgaaggttt
    39421 attcgatggg cggcatgaaa cagaccatca gtcgtgaaga gctgatagcg ctcggccagc
    39481 gtttcggcgt tacaccactc gccagtgcgc tggcgctgta aggagcaaac atgatccgca
    39541 aagcctttgt catgcaggtt aaccccgacg cccacgaaga gtatcagcgt cgtcataatc
    39601 caatctggcc ggaactggaa gcagtgttga aatctcacgg tgcgcataac tacgccattt
    39661 acctcgacaa agcgcgtaat ctgctgtttg ccacggtaga gattgaatct gaagaacggt
    39721 ggaatgcggt agccagtact gatgtttgcc aacgttggtg gaaatatatg accgacgtta
    39781 tgccaactaa cccggataac agcccagtca gtagcgagct gcaagaagtg ttttacctgc
    39841 cttaatgagt tgcggtgccg gaaaatatcc agcaccgcgc cttcatcaga agaagtattt
    39901 aaagcgaacg cgtgccccgt aacggtcatc agtatcatca ccgtgttttt tatcaccgtc
    39961 gctatccaga atggagtaat acgtcccgag gtaaatatta aagttctcca tatccatcac
    40021 attagcgaac tgataagacg catgaatggt gtgaatggtg tacgtccctt catcatcaat
    40081 ccagcagtcg ttatcacaaa ctacaccgct gaattcatca atcttattgt gcgcgtagat
    40141 ataaccgagc tcgaaacgtt tccacagcgc gttaatcccg gcagtgaaat ctttctcatt
    40201 attagcatct aaataggcgg tattaagatt aactacgacg ccattttccg gatccgtttt
    40261 caggccattc caggtcatgc tcataccata gccggtacga tcggactgat cgacaaaatt
    40321 acccttgcta tcggtataac cataagcatt atttactacg ttcgcttcca tcgctgcgga
    40381 aacggtgaat tcttccgtcg gcgaccaggc aattactgga cgcagataag caacattttt
    40441 ctgctgctcc atatcgcgtc catgataatt accgtcatta tataaagatg ttccgtcttc
    40501 aagtaacgtg tttaattcaa aataccagtt atcaagttgt ttgctgacga ggaaattacc
    40561 gccagcgtta gagcgcccgc ggccctcttt catcatatag atataaccgc tgccatcgtc
    40621 ataaagatcg ttcgcagtat taccggaatg ttcaacaaag gtatcctgat tcagcgggaa
    40681 catatcgtag gcttcaaaac gacctacttt gaccttccag tcgttttctt tgccaaagaa
    40741 gaataccgca tcatcgatat ttactgaacc gtgcatgtcc cccagcggtt gtgcggagaa
    40801 cccggcgaaa taaccattat ccagttttcg cataccatca aaacctaaca gaatacgacc
    40861 attgagattc cactgttcat tagtgggatc gctattcaca tcagcgttgg tcattgccag
    40921 caggccatga ttactttccg catccatatt aaattctacg tcgccgtaga tttttaaatc
    40981 gccataacct gagagcaaaa gattaggtga ggtggaattt ttctttgcag attccgccgt
    41041 tgcctgtcca tttaccggcg tattgccctg ggcagcctta atttcgcgga tctcagcggc
    41101 ttgttgtgtc tgcagctgtt taacctggct ctctgctttt gctgcccgca tttctgccgc
    41161 ttccaggcgt tcttccagca atgccatgcg ttgttccagc gttaaatgct gtgtttttgc
    41221 cattccggta ctacatgcca ataataaacc gatggctaaa gataatgtcg attttttcat
    41281 tatttacgtt ccctgaaata aaagagaccc tacgctttcc aaaaggaata cgtatataga
    41341 gaaatataag gtgtgcactt tcgaattttt aatccaactt cagcaggccg atggatagta
    41401 tcccgccata taatatgagt aaatccgctg atttcctgga acgtgaggaa tataacaata
    41461 gtgccgtttt taactaaata tcgtaaaaaa tattagatat gattcacgct gactcataag
    41521 ccacaaat
//
LOCUS       DAPHCV010000030        41384 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.030, whole genome shotgun sequence.
ACCESSION   DAPHCV010000030 DAPHCV010000000
VERSION     DAPHCV010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.030"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(22..98)
                     /locus_tag="RH033_001641"
     tRNA            complement(22..98)
                     /locus_tag="RH033_001641"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(62..64),aa:Met,seq:cat)
     gene            307..1404
                     /gene="mltA"
                     /locus_tag="RH033_001642"
     CDS             307..1404
                     /gene="mltA"
                     /locus_tag="RH033_001642"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HDV2990840.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGVDNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1531..1755
                     /gene="mltA"
                     /locus_tag="RH033_001643"
                     /pseudo
     CDS             1531..1755
                     /gene="mltA"
                     /locus_tag="RH033_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311700.1"
                     /note="membrane-bound, lytic; catalyzes the cleavage of
                     the beta-1,4-glycosidic bond between N-acetylmuramic acid
                     and N-acetylglucosamine residues; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
     gene            1831..2637
                     /gene="tcdA"
                     /locus_tag="RH033_001644"
     CDS             1831..2637
                     /gene="tcdA"
                     /locus_tag="RH033_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HDV2990841.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2688..3131)
                     /gene="csdE"
                     /locus_tag="RH033_001645"
     CDS             complement(2688..3131)
                     /gene="csdE"
                     /locus_tag="RH033_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HDV2990842.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(3131..4336)
                     /gene="csdA"
                     /locus_tag="RH033_001646"
     CDS             complement(3131..4336)
                     /gene="csdA"
                     /locus_tag="RH033_001646"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HDV2990843.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATTLKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPHLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4528..4755
                     /locus_tag="RH033_001647"
     CDS             4528..4755
                     /locus_tag="RH033_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HDV2990844.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            5106..6023
                     /gene="gcvA"
                     /locus_tag="RH033_001648"
     CDS             5106..6023
                     /gene="gcvA"
                     /locus_tag="RH033_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HDV2990845.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            6042..6437
                     /locus_tag="RH033_001649"
     CDS             6042..6437
                     /locus_tag="RH033_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HDV2990846.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6430..7530
                     /gene="rlmM"
                     /locus_tag="RH033_001650"
     CDS             6430..7530
                     /gene="rlmM"
                     /locus_tag="RH033_001650"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HDV2990847.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7574..8305)
                     /gene="fucR"
                     /locus_tag="RH033_001651"
     CDS             complement(7574..8305)
                     /gene="fucR"
                     /locus_tag="RH033_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HDV2990848.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(8363..8785)
                     /gene="fucU"
                     /locus_tag="RH033_001652"
     CDS             complement(8363..8785)
                     /gene="fucU"
                     /locus_tag="RH033_001652"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HDV2990849.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8787..10205)
                     /gene="fucK"
                     /locus_tag="RH033_001653"
     CDS             complement(8787..10205)
                     /gene="fucK"
                     /locus_tag="RH033_001653"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HDV2990850.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTDCHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQQLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQILIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTTQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(10314..12089)
                     /gene="fucI"
                     /locus_tag="RH033_001654"
     CDS             complement(10314..12089)
                     /gene="fucI"
                     /locus_tag="RH033_001654"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HDV2990851.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVRKPFVV
                     ATENDSLNGVAMLMGHQLTGTAQIFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGKPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(12122..13438)
                     /gene="fucP"
                     /locus_tag="RH033_001655"
     CDS             complement(12122..13438)
                     /gene="fucP"
                     /locus_tag="RH033_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HDV2990852.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     FGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            13985..14632
                     /gene="fucA"
                     /locus_tag="RH033_001656"
     CDS             13985..14632
                     /gene="fucA"
                     /locus_tag="RH033_001656"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HDV2990853.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14660..15808
                     /gene="fucO"
                     /locus_tag="RH033_001657"
     CDS             14660..15808
                     /gene="fucO"
                     /locus_tag="RH033_001657"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDV2990854.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPTLAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15863..16618)
                     /gene="xni"
                     /locus_tag="RH033_001658"
     CDS             complement(15863..16618)
                     /gene="xni"
                     /locus_tag="RH033_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HDV2990855.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVVGIGPKSATQLLVEFKSLEGIYENLNAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(16729..18096)
                     /gene="sdaB"
                     /locus_tag="RH033_001659"
     CDS             complement(16729..18096)
                     /gene="sdaB"
                     /locus_tag="RH033_001659"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HDV2990856.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHIKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(18154..19443)
                     /gene="sdaC"
                     /locus_tag="RH033_001660"
     CDS             complement(18154..19443)
                     /gene="sdaC"
                     /locus_tag="RH033_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HDV2990857.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPIIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            complement(19999..21363)
                     /gene="ppnN"
                     /locus_tag="RH033_001661"
                     /gene_synonym="ygdH"
     CDS             complement(19999..21363)
                     /gene="ppnN"
                     /locus_tag="RH033_001661"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HDV2990858.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            complement(21475..22323)
                     /gene="queF"
                     /locus_tag="RH033_001662"
     CDS             complement(21475..22323)
                     /gene="queF"
                     /locus_tag="RH033_001662"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HDV2990859.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            22391..22936
                     /gene="syd"
                     /locus_tag="RH033_001663"
     CDS             22391..22936
                     /gene="syd"
                     /locus_tag="RH033_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HDV2990860.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            23558..23887
                     /locus_tag="RH033_001664"
     CDS             23558..23887
                     /locus_tag="RH033_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HDV2990861.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDKPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            23887..24669
                     /gene="truC"
                     /locus_tag="RH033_001665"
     CDS             23887..24669
                     /gene="truC"
                     /locus_tag="RH033_001665"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HDV2990862.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDLIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            24687..25136
                     /locus_tag="RH033_001666"
     CDS             24687..25136
                     /locus_tag="RH033_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDV2990863.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDTLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEHWGTLLS"
     gene            25571..26923
                     /gene="gudP"
                     /locus_tag="RH033_001667"
     CDS             25571..26923
                     /gene="gudP"
                     /locus_tag="RH033_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HDV2990864.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            26925..28265
                     /locus_tag="RH033_001668"
     CDS             26925..28265
                     /locus_tag="RH033_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HDV2990865.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVKAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGQALNVPVCELLGPGKQRDAITVLGYLFYIGDRTKTD
                     LPYLENTSGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            28286..29626
                     /gene="gudD"
                     /locus_tag="RH033_001669"
     CDS             28286..29626
                     /gene="gudD"
                     /locus_tag="RH033_001669"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HDV2990866.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDKCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPLEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(29857..32613)
                     /gene="barA"
                     /locus_tag="RH033_001670"
     CDS             complement(29857..32613)
                     /gene="barA"
                     /locus_tag="RH033_001670"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HDV2990867.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            32670..33971
                     /gene="rlmD"
                     /locus_tag="RH033_001671"
     CDS             32670..33971
                     /gene="rlmD"
                     /locus_tag="RH033_001671"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDV2990868.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKALFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAIRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            34019..36253
                     /locus_tag="RH033_001672"
     CDS             34019..36253
                     /locus_tag="RH033_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDV2990869.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            36349..37140
                     /gene="mazG"
                     /locus_tag="RH033_001673"
     CDS             36349..37140
                     /gene="mazG"
                     /locus_tag="RH033_001673"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HDV2990870.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     GSAGNSREVLARWEQIKTEERAQKAQHSALDDIPSSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQVVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            37368..39005
                     /gene="pyrG"
                     /locus_tag="RH033_001674"
     CDS             37368..39005
                     /gene="pyrG"
                     /locus_tag="RH033_001674"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HDV2990871.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            39092..40390
                     /gene="eno"
                     /locus_tag="RH033_001675"
     CDS             39092..40390
                     /gene="eno"
                     /locus_tag="RH033_001675"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDV2990872.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(40450..41323)
                     /locus_tag="RH033_001676"
                     /pseudo
     CDS             complement(40450..41323)
                     /locus_tag="RH033_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
BASE COUNT        10242 a        10024 c        11003 g        10115 t
ORIGIN      
        1 tttgtcagca ttctaataaa ttggttgcgg gggccggatt tgaaccgacg accttcgggt
       61 tatgagcccg acgagctacc aggctgctcc accccgcgtc cgtggatgcg cactatactc
      121 tgctcgcgtt ttcatgcaac ctttttttta ccccgatcac ggatttggca tgattttgaa
      181 caaaaacaaa tcactaaagc ctattttttg tgcaaaattt gcgtcaggga gaggctgctc
      241 atttcattaa acgcggtggt ttgttatctt cgttgcgcct tattttttaa cctgaagaag
      301 agaacaatga aaggacgttg ggtaaagtac cttcttatgg gcacggttgt ggcaatgctt
      361 gccgcctgct cttccaaacc aaccgatcgc ggacagcaat ataaagacgg gaaatttacc
      421 cagcctttct ctctggtgaa ccagccagat gccgttggcg cgccgattaa tgccggtgat
      481 tttgccgagc aaattaacca tatccgtaat tcgtcaccgc gtctgtatgg caaccagagt
      541 aatgtttata acgcggtgca agagtggctg cgcgcaggcg gtgatacccg caatatgcgc
      601 cagttcggca ttgatgcctg gcagatggaa ggtgtcgaca actatggtaa cgtgcagttt
      661 acaggctatt acacgccggt aattcaggcg cgccataccc gtcagggcga gttccagtat
      721 ccgatttatc gtatgccgcc aaaacgtggt cgtctgccgt ctcgtgcgga gatctacgca
      781 ggagcgctga gtgataaata tattctcgct tacagtaact ccctgatgga taacttcatt
      841 atggatgtgc agggtagcgg gtatatcgac tttggtgatg gcagtccgct taactttttc
      901 agctatgcag ggaaaaacgg tcatgcctat cgcagcattg gtaaggtgtt gatcgaccgt
      961 ggcgaagtga aaaaagaaga tatgtcgatg caggcgattc gtcactgggg cgaaacacac
     1021 agtgaagcgg aggttcgcga gctgctggaa cagaacccgt ctttcgtctt ctttaaaccg
     1081 caatcttttg caccggtgaa aggggcaagt gcggtgccgc tggttggccg tgcttctgtt
     1141 gcctccgatc gttcaattat tccgccaggt actaccttac tggcagaagt gccgctgctg
     1201 gataataacg gcaaatttaa cggtcagtat gaactgcgtc tgatggtggc gctggatgtc
     1261 ggtggcgcaa tcaaaggcca acacttcgat atctatcaag ggatcgggcc ggaagccggg
     1321 caccgcgcag gttggtacaa ccactatgga cgtgtctggg tgctgaaaac ggctccgggc
     1381 gcaggtaacg tctttagcgg ctgatgtgat attctgtata ccgttttgac ggatacgaca
     1441 gggtgagggg taacctcaca tcctgattat tgactaagtg cgcgttgttc atgccggatg
     1501 cggcgtgaac gccttatccg gcctacaaat ctggcagaag tgccgctgct ggataataac
     1561 ggcaaattta acggtcagta cgaattgcgt ctgatggtgg cgctggatgt cagtggcgca
     1621 atcaaaggcc aacacttcga tatctatcaa gggatcgggc cggaagccgg acaccgcgca
     1681 ggttggtaca accactatgg acgtgtctgg gtgctgaaaa cggctccggg cgcaggtaac
     1741 gtctttagcg gctgatgtga tattctgtat gccgttttta cggatacgac agggtgaggg
     1801 ataacctcgc cctttttatt ctgaggtgct atgtctgtgg taattagtga tgcatggcgt
     1861 cagcgttttg gtggcacagc gcgtctgtat ggtgaaaaag cgttgcaact gtttgctgac
     1921 gcgcatattt gtgtggttgg tatcggtggt gtcggttcct gggcggcgga agcgctggcg
     1981 cgcacgggga ttggcgcaat cacgcttatc gatatggatg atgtgtgcgt caccaatacc
     2041 aatcggcaaa ttcatgccct gcgcgataac gttgggctgg caaaagcgga agtcatggcg
     2101 gaacgtattc gccagattaa cccggagtgc cgtgtaacgg tggtggatga tttcgtgacg
     2161 ccggataacg tagcgcaata tatgagtgcc ggttattcgt acgtgattga tgccattgat
     2221 agtgtacggc ccaaagcggc gctgattgct tattgtcggc gcaataaaat cccgctggtc
     2281 acgaccggtg gcgcgggtgg gcagattgac ccgacgcaga ttcaggttac cgatctggcg
     2341 aaaacgattc aggacccgct ggcggcgaag ttgcgcgagc gcctgaaaag cgattttggc
     2401 gtagtgaaaa acagtaaagg taagctcggc gtggattgcg tgttttctac tgaagcgctg
     2461 gtgtacccgc agtcagacgg tacggtgtgt gcgatgaaag ccacggcaga agggccgaag
     2521 cggatggatt gtgcatctgg atttggcgcg gcaacgatgg tgaccgccac ctttgggttt
     2581 gttgcggttt ctcatgcgct gaagaagatg atggcgaaag cggcgcgtca ggggtaaaca
     2641 atcaggtcgg atgcgacgcg aacgtcttat ccgacctggt tctgtgctta aacctgcttc
     2701 gccgcagcga taatcgcctc gcttaacgca ttcaacccct ggctgcgtga ggcgctaagc
     2761 tgcgcacgta atcccagctc atcaaacaat gccagtggtg actgtgcctg caactcggcg
     2821 gcggttttcc cctcaacggc agtcaacagc accgccagca ggccgcgcac tatacgtcct
     2881 tcgctgtcgc caaagaagtg cattttaccg ttttcagcca cggtatatcc cagccagacg
     2941 cggttttcgc atccggcaat ctctttagcc tgcgctttta actcgtctgg caaagccgga
     3001 agctgtttcc ccagcatgat caactggcga tatttatctt cccattgcgt caacggtgcg
     3061 aaggtattgc gtaacgtttc tgcggttacg gttgtgccga acggatgtcc agcgaattgc
     3121 gggttcgtca ttaatccacc aataattcca gcgcgcggtc aacggcattc accagcgcat
     3181 ccacatcact ctttgtatta tatggcgcaa aagaggcgcg cagtgtgccg gttacgccta
     3241 attctgccag tagcggctga gcgcaatgtt gcccggcccg caaggcaata ccgtactccg
     3301 ccagcagcgt caccatatcg ctgtgatgaa cgccagcaaa atcaaaggcc agcaggctgg
     3361 aatcctggca gcgaaatgag cggaagccag gacgttttgc cagcgcatct tccgccagcg
     3421 ttgctaagct acggctccag ctttcggcct ggttgatatc gtaatctgcc agccattcca
     3481 gcgccgcgct taatcctatg acaccagcga catttggcgt tccagcttcc agtttccacg
     3541 gcgcagattg agtcgtgaag ccgtcaaaac tcacttcgtg aaccattttg ccgccgccca
     3601 gccagggcga catcgcttcc agcagttctg atttgccata cagcacgccg atacctgtcg
     3661 ggccatacag tttgtgacct gaaaaagcat agaaatcaat atccagttgc tgaacatccg
     3721 cggggaaatg caccgccccc tgagcaccat caaccattac caccatcccg gctgaatgag
     3781 caaaggtaat cgctcgcgcc agatccgggc aaccgccagt gacgttcgac atttgtccca
     3841 gagcaagaat tcgactacga ggtgtgatca gttctggcaa caaatcgaca tccggcagtc
     3901 gctgcgcatt aagcggcaat ttcaccactt tggctccagt ttgttgggcg accatcagcc
     3961 aggggacgag gttggcgtgg tgttctgcca cgctgacaat aatctcatcg cccggttgca
     4021 gatgcggacg cgcatagcat tgcgccacca tgttgatgga ttcagtggtg ccgcgcgtcc
     4081 agacgatagt tttgtcatct ggtgcattca gtaattgcgc cactttctcc cgtgcagctt
     4141 cataacgcgc ggtcaggcgt tgggcttcgg caaactggct gcgatggacg tttccggcgc
     4201 tcaggctata aaactgtcgg gtggcttcaa ccacggcttc aggtttaagc gtggtcgcgg
     4261 cgctgtcgag atagacgccc gcatcctgta gcgcgggaaa ctgggcgcga aactgcgcgg
     4321 gattaaaaac gttcatggta ctcctcggct tgatgcaccg atcgtgacgc aaaattcact
     4381 ttgatacaag gagagtgcta accatcagaa ttatctggat atcctggcga agccgcgtca
     4441 gtaacatatg ctgaatagtg ttaggcgtta attttagcct gttaatcaat actattaaga
     4501 tttattaatc gctttaagga gaagatgatg aaaaagactg ccgcaattat ttctgcctgt
     4561 atgctgactt ttgccctgag cgcctgttcc ggttcgaact atgtgatgca caccaatgac
     4621 ggacgtacca tcgtctctga cggcaaacca cagactgata acgataccgg tatgatttcg
     4681 tataaagacg ctaatggcaa caaacagcag atcaaccgta ctgacgtgaa agagatggtc
     4741 gaactggatc agtaattcgc gatcgcaagg taaaaaaaag caccgcaatt aggcggtgct
     4801 acattaatca ctatggacag acagggtaaa tgtacaggaa gtgaaaaaag gtagctttgc
     4861 taccatggtc tgaatcgcag accaattgca aacacaacaa cacaacatca caaccgtaag
     4921 ccaaaagttc accagaacac gcattccgat aaaacttttc gttccggctc aggaagtgcc
     4981 gccactatag gtatttgctg gtagaagctc aacggacaat ttataatggc tcagattaaa
     5041 aaaactaata ggttacacag tgtgatctaa ttgttaaatt catttaacat caaagtttaa
     5101 aagccatgtc taaacgatta ccaccgctaa atgccttacg agtttttgat gccgcagcac
     5161 gccatttaag tttcactcgc gcagcagaag agctttttgt gacccaggcc gcagtaagtc
     5221 atcaaatcaa gtctcttgag gattttctgg ggctaaaact gttccgccgc cgtaatcgtt
     5281 cactcctgct gaccgaggaa ggtcaaagct atttcctcga tatcaaagag atattttcgc
     5341 aattaaccga agcgacgcgt aaactccagg cccgtagcgc taagggggcg ttgacggtca
     5401 gtttactccc cagtttcgcc attcattggt tggttccgcg actttccagc tttaattcag
     5461 cttatccggg aattgacgtt cgaatccagg cggttgatcg tcaggaagat aagctggcgg
     5521 atgatgttga tgtggcgata ttttatggtc ggggcaactg gccggggcta cgggtggaaa
     5581 aactgtacgc tgaatattta ttgccggtgt gctcgccgct actgctgact ggcgaaaaac
     5641 ccttgaagac cccggaagat ctggctaaac atacgttatt acatgatgcg tcacgccgtg
     5701 actggcagac atatacccga cagttggggt taaatcatat caacgttcag caagggccaa
     5761 tttttagcca tagcgccatg gtgctgcaag cggctatcca cgggcaggga gtggcgctgg
     5821 caaataacgt gatggcgcaa tctgaaatcg aggccggacg tcttgtttgc ccgtttaatg
     5881 atgttctggt cagtaaaaac gctttttatc tggtttgtca tgacagccag gcagaactgg
     5941 gtaaaatagc cgcctttcgc caatggatcc tggcgaaagc cgctgctgaa caagaaaaat
     6001 tccgctttcg ttatgaacaa taatttacgt agggtacgac catgaccagc cgttttatgc
     6061 tgattttcgc cgccattagc ggcttcattt ttgtagctct gggcgctttt ggcgcgcatg
     6121 tgttaagtaa aaccatgggg gccgttgaga tgggctggat ccagaccggc ctcgaatacc
     6181 aggcgtttca tacgctggcg atcttaggtc tggcggtggc aatgcagcgt cgcatcagta
     6241 tctggtttta ctggagtagc gttttcctcg cgttaggcac ggtgctgttc agcggcagcc
     6301 tttattgcct ggcgctgtcc catctgcgtt tgtgggcgtt tgtcacaccg gttggcggcg
     6361 tgagcttcct tgcgggctgg gcgttaatgt tagttggtgc tatccgttta aagcgcaagg
     6421 gcgtaagtca tgaataaggt tgtattgctg tgccgtccgg gctttgaaaa agagtgcgcc
     6481 gcagaaatta ccgataaagc cggccagcgg gaaattttcg gttttgcccg cgtgaaagag
     6541 aatgcgggtt atgtcattta tgaatgttat caacctgatg atggcgataa gttaatccgt
     6601 gagctgccgt tcagttcatt aatttttgcc cgccagtggt ttgtggtggg ggaactcttg
     6661 cagcatttgc cgccagaaga tcgtattacc cccattgtcg gcatgttgca gggcgtagta
     6721 gagaagggcg gtgaactgcg tgttgaagtt gccgatacca acgaaagcaa agagttgctg
     6781 aaattctgcc gtaaattcac tgttccgcta cgcgctgcct tgcgcgatgc tggggtgctg
     6841 gcgaactatg aaacgccgaa gcgtccggtt gtgcatgtat tctttattgc accaggctgc
     6901 tgctataccg gttactcata cagcaacaat aattcgccgt tctatatggg cattccgcgc
     6961 ctgaaatttc cggcagatgc gccgagtcgt tccacgctca aactggaaga ggcatttcat
     7021 gtgtttattc ctgcggatga gtgggatgaa cgcctggcga acgggatgtg ggcggtggat
     7081 ttgggcgctt gcccaggcgg ctggacctac caactggtga agcgcaacat gtgggtttat
     7141 tccgtcgaca acggcccgat ggcgcaaagt ctgatggata ccggacaggt gacgtggctg
     7201 cgggaagacg gtttcaaatt ccgtccgacg cgcagcaata tctcctggat ggtatgcgat
     7261 atggttgaaa aaccggcgaa agttgcggca ttgatggcgc agtggctggt taatggctgg
     7321 tgtcgtgaaa ccattttcaa cctcaaactg ccgatgaaaa aacgctacga agaagtgtca
     7381 cacaatctgg cgtatattca ggcacagctt gatgaacatg gcataaatgc tcagattcag
     7441 gcgcggcagt tgtatcacga tcgcgaagaa gtgacggtgc acgtccgccg catctgggct
     7501 gcggtgggtg gtcgtcgcga cgagcgataa caggcaaggc cggggcgtcg cctccggcct
     7561 gctacccttt ccgtcaggct gttaccaaag aagttgcaac ctggcgctca tcagaaataa
     7621 tgtgcgttac ctcatccaga tgcccgatgc gggcttcccc tgaacgatta aatttacttt
     7681 tatcaatcaa taacaacgat tgcgcggcac gttttaatag catcgatttg taatcagcgt
     7741 tgatggcatt ggagtcccac agtgcgccgc tgctatcgat cccttcacaa gaaaaaataa
     7801 acagatcgat ttccagcgat ttaagttggg aaatcagcga gggattgacg taacagccat
     7861 atttgcgctc aagtgtgccg ccggaactga tcagttgaat gcgttcgcgt ttaccgagtt
     7921 catggcaaat cggatggcta ttggtgaaga cctgaatgtt gatgtcaggc aactggcgtg
     7981 ccagatacca gcaggttgaa ctggcgtcta aggctatcac catcccttct tcaatccacg
     8041 cgagcgcctc gcgcgcgata tctgctttat gcgcataatg gcttttcagc cggatgtgaa
     8101 agggatcgcc actgtcttga ttttgacggt ggatatattt agcgcgtcca tgattgcgca
     8161 gaattttacc ctgcgtctgt aattcattga gatcgcgacg aatggtttct ttactgacct
     8221 ttagctgttc agagagagct tccgtggtca ggctggtatg gttcagcagc aggtcgacta
     8281 tcgcttgctg gcgtgccgct ttcatatttt tcaccccatc attcgataac cgcccgcgtc
     8341 atgcgggcgt accggtatga gattacggtg ttaccccttt ttttaaaaga atattcccgt
     8401 acttcgctcg ttcgcctgtg ataacgatcg caaaggcttt ttgcgcccgt tcataaaacg
     8461 caaaacgatt gatgcggatg atgtcaggac acggggcttg tagtgaaagc gcattacggt
     8521 aacgtcgttc tacttcagga tcgagagtgt caccttctac cgccgccatc atcaccagcg
     8581 gtggtgcata actgtccagc tcaaataacg ggataatcgc ctggagcaag tcgctcacca
     8641 acaggccatc agcgcggatc acctgcggtc ccatcgaatg ggcgggaaag tgagcatcgg
     8701 aaaaaataat ttcatctcca tgccccatct ctgccagcac tttcaatagt tcgggagaaa
     8761 ttaacggcga aattgttttc agcatttcac acttcctcta taaattcagg ttcagtttgc
     8821 gggtagaaat aacggtactg ataatgaatc tgtgcacggg cttcttccgg gctgttaaat
     8881 tcccctacgc cataccaacc aaacagcgca gctcctgcga cggtcgtttc ggcatcgtcg
     8941 agaactttta ccggaatatc aagcatattg gctttaatct gattccacaa tgtgttgcga
     9001 cttcctccgc cgactaacaa taattcagag gctttaaagt gcccgatttt ttccagcatc
     9061 tgtagattgc gctgtaattg cgtggttaac ccttccagcg ccgcgcgata gaaatgcccc
     9121 cgcgtggtat taagcgttac gccttgccag ccagcgttct ggcacgacaa taaatcacac
     9181 tgcattttca ctccatctgc gccaggcgcg atcaggcgag cttcttcaat caatatttgc
     9241 cagggagttt cagccgtcca gaacagtttt ctcacccatt ccagcacgcc ggatgccagc
     9301 cattgcatac ctgggttata caaccctgcc tggctatcca gttcgcaggt agaaccggcg
     9361 tactgactta acagcgaagt atcaacctgg gcgctgcgga ccattaaaat ttcccatgta
     9421 ccggaagaga gcacgggttc attttgttcg gcaccagcgc caaaaagggc gaactgggta
     9481 tcgtgacctg cggaaatcac cggtatgcca acgggtaagc cgagcattgc tgcggcgctg
     9541 ttctgtagcg taccaatctg ttcacccgct tccaccagac gagggaagag tcggcgtgga
     9601 ataccggtgg cttgtaaaat ttgcggactg aaatcgcgtt gctggatatc cagcatctgg
     9661 ctggttccgg ccatcgtaat atcagtagtg aattcgccgg ttaaacggtg gttaatcagt
     9721 gacgaaataa agagccaggc gtgcgcgcgt tccagcagtt gtggatgatt ttctttcaac
     9781 cacaccaact tatataacgt attgaaacta aaggctccga ctccagaaat agcctgcaac
     9841 tgctgtgcgg agatcaaccg ttcaatattg tccattaccg cggctgttcg cggacatttc
     9901 cagctaatga tcggatagag cagattgcct tgcttatcta ccagagcgcc atccacacca
     9961 aaggtggtga cggcgatacc gcggatatgg caatcagtca gttcactatt gatttgccga
    10021 cagcaatcag caaagcgttg caaaatggcg tctaaagacc actggtgcca ggtgttgttt
    10081 tccatcgcga tatcgctggc attaggcgtt gaggcgcggg caacaatttt tccctgccga
    10141 ttaaccgcga tggccctgac attggtcgcg ccacagtcga gtaccaggat aacttcttgt
    10201 ttcataatcg ctcctgcaat atagccggat aacattgctt atccggctaa ccactcttgc
    10261 ttgttcaggc ccggtagccg gagcgaccgg gcatcacatc agggagtcat gtattaacgc
    10321 ttgtacaacg ggccgtagtt ctggcaagcg cggtaatcct ggccttcaat atccatgccg
    10381 tgcgcggccc aggcagaagg acgatacact ttggtctctt caacgttgtg catacatacc
    10441 gggatacgca gcatggaggc aagagtgata aagtctgcgc caacgtggcc gatggtcaga
    10501 actccgtggt tagcgcccca gttcgccatt accgagtaca catccgtaaa cggcccttta
    10561 ccggtgaggc gcggtgcaaa ccaggtggtt ggccaggttg agttggtgcg tttgttgagg
    10621 atgtcatgca catccttcgg caattccacg ctccagcctt ccgcgatttg cagtaccggt
    10681 cccaggcctt tgatgatgtt gacacgagtc atggtgaacg ggacgccgcc ttcggtaagg
    10741 aagcgggaag agtaaccgcc gccacggaag tattcgtgga tcgccgggca ccattcggta
    10801 gcggcgaggc aagcgtcagc ctcttgctga gagatttccc agtgcggctt catcgtcggt
    10861 ttaccttcgc tgtcgcgttg tttacaggag ccgtccagcg cagcagaacc ggagttgatc
    10921 aaatggatga tgccgtgttc tgccagcccg tccagtttat gccccgttac acgctcaatt
    10981 gcttctggtg accagtaggt acggacatcg gcaaatatct gagcggtgcc ggtgagctgg
    11041 tggcccatta acattgccac gccgttaaga ctgtcgtttt cggtcgccac gacaaacggt
    11101 ttgcgcacgc cattccagtc aaatgaactg ttgaggatcg cttcagcggt atcgccattg
    11161 ggatattgat cggtccagtg acgttgcccc tggaagcctg cagcgatggc gttgtagcca
    11221 agtgattctt ccacgcggcc aatatcggcc agtttgctgt tgccttgcat catgtcgcgg
    11281 atacacatcg ccatcagtaa actttcgcgc agaactgcgc ggctttgctc ggcattgcgt
    11341 tgatactgtt tgttattttc atcttcgcca tagcggaagt ttttatcagc ccaggccagc
    11401 gccatttcca gttcggcttc gtcgtaaatc ttctgatcga tacggcgacg cagttcggtc
    11461 atatccaccg cctggacttt cattcccagc caggattcaa agaagttgtg atcaacgatg
    11521 gaaccggcga tacccatcga aacgccgccc agcgacagat agcttttacc tttcatgctg
    11581 gcgacggcca aaccggcgcg ggcaaagcgc agcagttttt cttcaacatc ggcaggaatc
    11641 gatgtgtcat cggcatcctg aacgtcatga ccgtaaatgg agaatgccgg gatgcctttc
    11701 tggctgtgag ctgccagagc cgctgccagg taaacagcgc cgggacgttc agtgccgtta
    11761 aagccccaaa tggctttcgg gcgggttgga tccatatcga tggtttcact gccatagcac
    11821 cagcaagggg ttaccgtaat ggtgaggcct acattctgac tgctgaattt ttcttcgcaa
    11881 gcagcggctt cagccatacc cgcgatacag gtatcggaaa tgacacactc gacggcagct
    11941 ccacaggcat ggcgcagttt ctcggtcagc agtgcagccg tggctttcgc catattcatt
    12001 gtttgttctt caagcgactc acgaacaccc atgcgacgac cgtcaataac cgggcggata
    12061 ccaattttcg gtaagctgat ttttttcatt acagattcct cactttattc ggaaaatatg
    12121 ttcagttagt tgctgtttga gaacggaaac gggcaaagat aaagatgacc gcgaagcaga
    12181 gtgcggggat cagttcagca gtggggatgt tgcccgccgc gtcactgaca aaacccatga
    12241 ccggagtgac aataccgccg ccaatgatgg tcataacgat gaaggacgaa ccgtatttgg
    12301 tgtcctggcc gagattctta atgcccagcg agaagattgt tgggtactga atcgacataa
    12361 aggcgctgca taaagtcagg gctattaagc ccacatgacc gccagcgaag gctgagatca
    12421 ggcacagtgc catagcgatt aatgcgtagg cggccaggac tttgtgtggt gcgaagcgac
    12481 tgatgagcca ggtaccggtg aaacgaccaa taaagaagca caccatggtt ccggttaaat
    12541 agttagcggc aaagcctgct gtcatacctg gaatttcttc tacagcgtag cgaatcaaat
    12601 agctccagca ggctgtttgt gcgccgacat agcagaattg cgctaatact gcccagcgcc
    12661 agtggcgaat acgcgccagg cgagaaagcg atgcggagaa cgatccttgt ttggcgtcac
    12721 tgtgattatc actctgcaat gccgggaatt tcgtcagcat gatcagcagg gcgaccagta
    12781 acacgatagc cacgatgatc atataaggtg tctgtaccga taataccagg ctgtgtttat
    12841 acgcactcaa ttgctctgga gacattttat cgagaacgtc ttgcgattga tgtggcacgt
    12901 tagacaaaat aagactttgc ccaaagacaa ccgcgataat tgcgccaaat gagttaaatg
    12961 tttgcgcaag atttaagcgg aagtgaccac tgctttccgg ccctaatacc gtaacaaaag
    13021 ggtttgcggc agtttccaga caacctaatc cggctgcaat aataaatagg ccaactaaaa
    13081 acaaggtgta gttcattatt tctgcggcgg gccagaataa tgcagcgccg aaggcatata
    13141 aaaacaatcc ggtaataatc cctgctttat aactgagttt tttcatcaat atcccagcag
    13201 ggattgggat aatgaaataa ccaaagtaaa aggccgattg gatcaggcca gcctggaaat
    13261 ttgtcagcgt aaaagcctgc tggaattgag gtaataaaat gtcgttaagg ttattggcta
    13321 ccgcccaaag aaaaaacagt gagcacagca gcgcgaatgg aataatgtaa cttctgcttt
    13381 gccctgcatc tttatctacc gcacggtaac tctgcgtttg tattgatgtg tttcccatag
    13441 catcctctta ggttcagaag cttaagcacg taatcggtta tgttttcaaa agtgcaaaac
    13501 cagcgagttc gggcttcata tcagtgaata tttgcctgtc agaaatgaac tcatggattc
    13561 attttcaaat aaaaaacgct ctgtaatatg acggcggtca cacttattag ttgggaggtg
    13621 actttatgtg actaccatca ctttaataat cgaaatttca tgcccgtttc agaaaataaa
    13681 tcataaaaaa cacaaacccg atgaccgtat taattgtttg tgccgaaaac gggcatttct
    13741 ggcgaggggg aatggaaaaa tgaccgtttt gataaatatt tttaagagcg gtcattttta
    13801 atagggttat aaaaagagaa cttgcagtta tttactgtga ttagttgaac caggtcacaa
    13861 aatataagct tgtgaatttc tttctggtta atcaggaaga agatggtggg taattaaacg
    13921 gctaattcaa tagtgtgaaa ggaacaacat tattgccctg ttttgaatca gagagaggta
    13981 gctaatggaa cgaaataaac ttgctcgtca gattattgac acttgcctgg aaatgacccg
    14041 cctgggactg aaccagggga cagcgggtaa cgtcagtgta cgttatcagg atgggatgct
    14101 gattacgcct acaggcattc catatgaaaa actgacggag tcgcatattg tctttattga
    14161 tggcaacggc aaacatgagg aaggaaagct cccctcaagc gagtggcgtt tccatatggc
    14221 ggcctatcaa agcagaccgg atgccaacgc ggttgttcac aatcatgccg ttcattgtac
    14281 ggcagtttcc attcttaacc gaccgatccc cgcgattcac tacatgattg cggcggctgg
    14341 cggtaattct attccttgcg cgccttatgc gacctttgga acacgcgaac tttctgaaca
    14401 tgttgcgctg gctctcaaaa atcgtaaggc aactttgtta caacatcatg ggcttatcgc
    14461 ttgtgaggtg aatctggaga aagcgttatg gctggcgcat gaagttgaag tgctggcgca
    14521 actttacctg acgaccctgg cgattacgga cccagtgcca gtgctgagcg atgaagagat
    14581 tgccgtagtg ctggagaaat tcaaaaccta cgggttacgg attgaagagt aatttcgtaa
    14641 agcaacaagg agaaggatga tggctaacag aatgattctg aacgaaacgg catggtttgg
    14701 tcggggggct gttggagctt taaccgatga ggtgaaacgc cgtggttatc agaaggcgct
    14761 gatcgtcacc gataaaacgc tggtgcaatg cggcgtggtg gcaaaagtga ccgataagat
    14821 ggatgctgca gggctggcat gggcgattta cgacggcgta gtgccgaacc caacaattac
    14881 tgtcgtcaaa gaagggctcg gtgtattcca gaatagcggc gcggattacc tgatcgctat
    14941 tggtggcggt tctccacagg atacttgtaa agcgattggc attatcagca acaacccgga
    15001 gtttgccgat gtgcgtagcc tggaagggct ttccccgacc aataaaccca gtgtaccgat
    15061 tctggcaatc cccaccacag caggtactgc ggcagaagtg accattaact acgtgatcac
    15121 tgacgaagaa aaacggcgca agtttgtttg cgttgatccg catgatatcc cgcaggtggc
    15181 gtttattgac gctgacatga tggatggtat gcctccagcg ctgaaagccg cgacgggtgt
    15241 cgatgcgctc actcatgcta ttgaggggta tattacccgt ggcgcgtggg cgctaaccga
    15301 tgcgctgcac attaaagcga ttgaaatcat tgctggggcg ctgcgaggat cggttgctgg
    15361 tgataaggat gccggagaag aaatggcgct cgggcagtat gttgcgggta tgggattctc
    15421 gaatgttggg ttagggttgg tgcatggtat ggcgcatcca ctgggcgcgt tttacaacac
    15481 gccacacggt gttgcgaacg ccatcctgtt accgcatgtt atgcgttata acgctgactt
    15541 taccggtgag aagtaccgcg atatcgcacg cgttatgggc gtgaaagtgg aaggtatgag
    15601 cctggaagag gcgcgtaatg ccgctgttga agcggtgttt gctctcaacc gtgatgtcgg
    15661 tattccgcca catttacgtg atgttggtgt acgtaaggaa gatattccga cactggcgca
    15721 ggcggcactg gatgatgttt gtaccggtgg caacccgcgt gaagcaacgc ttgaggatat
    15781 tgtagagctt taccataccg cctggtaaat gcgctgatgt gataatgccg gatgcgacgt
    15841 ttgcgccgta tccggcacgc cgttaccgta ccaaccgcaa ttgctgtaaa ttgccgtcga
    15901 tatgcaaatc ggtttgtaag cgggcaatat cgcggcacag aaacgccatc tctttatggg
    15961 tttctaattt tttgcgccac ttttcggcaa ccgcattcag attctcatat atcccttcca
    16021 gactcttaaa ctcgaccagc agctgcgtgg cgctttttgg tccgatcccc acaacacccg
    16081 gtacctttga actgctgatc cccgcaagtc cccagtaatc gggcaactgc tgcggttgaa
    16141 cgccaaattc tttatcgata aatggcgcat ccagccaacg tttctggaag taatcacgaa
    16201 tacgtaatgt cggtgaaagt aactgacagt agcctttatc tgtcgaaaca atcgttgcct
    16261 gatgcccggc ctgtgtcact ttgaccgcca gcgtggcggc taagtcatcg gcttcgttgc
    16321 cgctggcgga ccagcacggg acgccgcgtt gctcaaaggc ggcgcgtaat gcaggcatct
    16381 cgtcgtgcaa ctcttccggc attggcggtc gacccgcttt gtaatctggt aaacgctgat
    16441 gacgccagcc gctactgcgg ttttcatcat caaaaacggc gaccgcgtgg gttggctggc
    16501 tgtgcataat gagctgatcg agcgcgtgct ggcaggtttc gacacagggc gaaccctgaa
    16561 cggcatgaat gcgacgaata agattcagtg catcgacaat aagcaaatga acagccacga
    16621 taaccccctt aaagatcagg cgataagggt aacattcgat gccggaacag gctatggctg
    16681 gttgagaaaa cgggaagagg cctcgcaaaa cgaggcctcc gggagagatt aatcgcaggc
    16741 aacgatcttc attgccaggc caccgcgaga ggtttcgcgg tacttggcgt tcatatcttt
    16801 acctgtttcg tacatggtct cgataacttt atcgaggcag acgcgcggct cgctggtacg
    16861 gcgcagcgcc atacgcgcgg cgttcaccgc ttttactgcc gcaatggcgt tacgctcgat
    16921 gcacggtacc tgcacctgtc cagcaaccgg gtcacacgtc agaccgaggt tatgctccat
    16981 ggcgatttcc gccgcgatgc acacctgcgc cgggcttgcg cctaacagtt ctgccagacc
    17041 agctgccgcc atagagcacg ccacgccaac ttcaccctgg caacccactt cggcaccaga
    17101 aatcgacgcg ttcatcttat aaagagaacc aatggcgctg gctaccagca gataacgagc
    17161 cagtgagtta gcgttcactt cgcggataaa cttatcgtag tacgccagca cagccgggat
    17221 aatcccgcac gcaccgttag tcggcgcagt caccacgcga ccgccagcag cattctcttc
    17281 gttcactgcc agtgcaaaca tgttgatcca gtcaacaacc gccatcgggt cagtggtggt
    17341 tttatcctgg ctgaccagca tccggcgtag tgccgcagca cggcgtggaa cgcgcagttt
    17401 gccaggtaac acgccttcgg tagaaatacc acgctcaata ccgccacgca tgacttccca
    17461 gacgttcgcc aggtgctgtt ccagctcttc tttgatgtgc agcgccagct cgtttttcat
    17521 catcaggcca gagagtgaca gcccggtttc ctgacaatgt ttttgcagat cggctgctga
    17581 actgtacgga taaggaactt caaccggtgc gctatcctgc tggccaaaat gttcttcatc
    17641 aacgataaag ccaccgccga tagagtagta agtctggctg taaacgactt tatcgcccgc
    17701 cagtgcggta atgcgcatac cgttttcatg cagagaaagg ttgtcggcat ggaagttcat
    17761 gcactgatca accgggaact ccacttcatg ctgaccgttt gccagcatca ggcgaccatg
    17821 agtattcaca tcctgaataa aaccagggat ggaatcgata tccacggtat ccggcaggtt
    17881 acccgccagg cccataataa tggcgatatc agtgtggtgg cctttaccgg tcagagagag
    17941 cgagccgtac acgtcaacca ccacgcgggt cacgtcttta agcaggttac gggcaatcag
    18001 atcgtcggta aattgtttac ccgctttcat tggtccaacg gtatgagaac tggaagggcc
    18061 aatgccgatt ttgaaaatat cgaatacgct aatcatagga aatacatcgc gttaaaacgg
    18121 aggaagcgcc gcccgaaagc ggcgcgaaag gacttagctg aacagagagt agaagattgc
    18181 ggagattgca atcagaccca tcacgacaac gaatacgttg ctgatgtgac cgctgtactt
    18241 acgcattgcc ggtactttct gaattgcgta catcggcatc aggaacagga tcatcgcgat
    18301 gattgggccg cccagggttt caatcatacc caggatgctc gggttcaggg tggcaacaat
    18361 ccaggtcgtt accagcatga acagcgcagt gatacggttc agcttgttga tttcgataga
    18421 cttaccttta ccacgcagag atttaatcac cataccgttg aagccttcac gtgcgcccag
    18481 gtagtggccg aggaaggatt tggtgatagc gataatcgcg ataatcggag ccatccacgc
    18541 gatgatcggc gcgttaaagt ggttagccag gtaagacaga atcgagatgt tctgctcttt
    18601 agccgcagcc aggtctgctg gagtcaggct cagtacacag ctgaagacga agaacattac
    18661 ggtcagcacc atcatgatgt gtgcgaatgc caggatcttc gagcatttct gttctgccat
    18721 atcgccgtac tcttcacgct tcgcaacggc gaaagaagag atgatcggag agtggttgaa
    18781 cgagaacacc attaccggaa ttgccagcca cagggtcatc caaagaccgt ttccggttgc
    18841 agatgcagtg tccagagaca gcgtttccag tgctgcgccg ttccactgcg ggatcaggta
    18901 cagagccagc agcatcagta cgccaacaaa cgggaatacc agaatactca tcgctttaac
    18961 gatcatctgc tcaccgaagc gaacgatggt catcataccc acgatcagga tcagcgacag
    19021 aatcgcacgc ggcggtggtg tcatacccag ctggtgagac atgaagcttt caactgtatt
    19081 ggtgattgcc acgctataaa ccagcagaat cgggtagata gcgaagaagt agagcagggt
    19141 aatcagttta cctgcgccaa taccaaagtg ttcttctaca acctcggtga tgtcttcgcc
    19201 cgggttttta ccagacagta cgaagcgagt cagaccgcgg tgagcaaaaa atgtcatcgg
    19261 gaacgcaagg atagccatga tgatcagcgg gatcataccg ccaacaccgg cgttgattgg
    19321 caggaacagc acgcccgcgc cgattgccgt gccgtaaagg cccagcatcc acatggtgtc
    19381 tgtcttgcgc caggcactac gagagtcttt cgacgcaatc gtgctggttt gagtcgtttc
    19441 catctatttc tcctggagga atgcaaataa ttcagctttt agccagttca gcccgggaag
    19501 gtgcctgacc gtaaaaatga aatctgtcta taggcgatgg cttaatcatt taccgccgta
    19561 ataatttgag ggcggaaaga tacacacaag ttatgtatct atcagtgatc ttgatcccaa
    19621 atacaataat ctacttggaa gatagattat cccaacgctt tactctgtaa atcaaaagta
    19681 aaagagaatt taagggcaat atttgcccat taaacgacat ctgacttctt ccggggcgcg
    19741 caatatatgt aggttgaact gaaaaaatca atacttttag ttaatcatgg cgataatttc
    19801 tttttcatag cgataaaatg tggtttatga aaatagggaa acgtttgcgt tacaacataa
    19861 attgtatgac atattaactg taatgaaatt tatcgcatga gggtggtaag aaacattcat
    19921 ataacaaatg aaataaattg atgggtatgt gaccaaaaac gagaaaaccc ggcaacaaac
    19981 gtcaccgggg gagggagatt acgtgcagat ttcgtagcaa gggatgtagg ctgagcctgg
    20041 caacttcata cgatgctggg caacaaaacc ctgtagcagg tcatccatac gacgcataat
    20101 ctctttatcg ccgttgattt tataaggacc aaactcttca atggcgcgaa taccgacttc
    20161 ttttacgtta cccgccacaa taccggagaa cgcacggcgc aggtcggcag ccagcacttc
    20221 aacgggttga tccgggtaaa gcttcagatt agccatattc tcgtgagacg gctcaaacgg
    20281 catttgcaaa tctggcgcaa tgcgcattga ccagttaaag ctgtaggcat cgcctgtgtc
    20341 gcggcgattt tctttcacca gcggcatcga ttttttcatc tgacgagcga cttcagcggc
    20401 gtcatcaatg atgatgcggt aatggcggcg cgcgttttca cccaatgtat gcacgacaaa
    20461 ctcgtccagt acgcggaagt agtcggcgct ctctttcggg ccggtgagga tcaatggtaa
    20521 aacctgatct ttgttggccg ggttcattaa aattcccagc aaatacagca actcttctgc
    20581 cgtacccaca ccgccaggga agataatgat accgtgagcg atacggacaa acgcttccag
    20641 acgtttttcg atatccggca tgatgatcaa ttcgttgacc agcgggttag gcggttcagc
    20701 ggcgataatc gacggctctg tcataccaat aaaacgactg tctttgtaac gctgctgtgc
    20761 gtgtccgacc gcagcgcctt tcatcggcgc ttccatcgct cccggaccac agccggtgca
    20821 gatattcagc tcacgcaggc ccagctgatt cccgacgcga cgggcataca aatactcgtt
    20881 ttcgttaatt gagtgcccgc cccagcagac caccatattt ggcgcttcac cgacatgcag
    20941 tgcgcgagcg ttacgcaaga tggaaaagac caggttagtg atgtggacgg agttgtcgag
    21001 attcaggttg ggaaaacgaa cggtgttatg gatttgcccg taaacgaaga gaatgtcacg
    21061 cagaaccgcg aacaagttgg cctgcaaagc gcgaataatt cgcccatcga caaaagcctc
    21121 ttccggggga tttatcagtt ccagctttac gccgcgttca cggcgcagga cgttaatatc
    21181 gaaattttca aaacgagaca gcaattcttt gctgttatcg gtcaaactac cggagttcag
    21241 tacggcaagt gaacagttgc gaaacagttg atagaggtcg ctgctggcgg tgcgtttaag
    21301 catatccact tccagctgcg acaacatatc catggagcca agcgggctaa tatgtgtaat
    21361 caagaaaaac tccttatggg acgaatatcg tcattccctt tgattacaat agccctgcct
    21421 gatgaccttt cacaaccttc tgcgtgaatc cagcacgcaa aattgagaaa aaatttattg
    21481 ccgaaccagt cttgtggtcg atgggacaaa atcgctatta ctgcgccacg ggttaatgtc
    21541 cagaccgcca cgacgggtat aacgtgcgta aacgctcaat ttctctggct ggcagaagcg
    21601 taacaggtca ttaaagatgc gttccacgca ctgttcgtgg aactcgttgt gatgacggaa
    21661 agaaaccagg taacgcagca gtttttctct gtcaatttgg cgtccacgat actgaatttg
    21721 gatcgatccc caatctggtt gatgggtgat caggcaattt gatttcagca ggtggctgac
    21781 gagcgtctct tctactactt tttcgccact ggtggcattc tccagatagt cagtagtgaa
    21841 ttcatagtta tcgatagtga tatcctggtc atcaatgcaa gtgccattga aatgacctat
    21901 cggttggcct tccagttcat caagacgata taacgccacg ctaaccttac cctgagcgca
    21961 agtgcttaag tcgcgctcca gcgtctggcg cacctcatcc cagttattaa aacgcgtctg
    22021 gttaaaactg ttgagataga gcttaaaact cttcgactca atcagatttg cgctggtgta
    22081 atcaagttca acatgaccga cagcgacctg cggcaaacct ttcgcattca gccaggaaag
    22141 ttcatacagc gtccagatat ccgtaccgtg aaaaggcagg ttatccgctt tcagacccag
    22201 cgggtcgcga ttcaggctgc gtggaacgcc ttgcagtagg ctggcgtcat aggtatcccg
    22261 gtaatcggtt gattttccaa gagtcaggcc cgcaagtgcc tgatggtttg cataagaaga
    22321 catgtttcac cgtcataaac cagataaact aaacgacaag tgtatctggt ttaagtgaag
    22381 agagaaatta gtggacgatt tgaccgcaca agccctgaaa gattttactg cacgctactg
    22441 cgatgcgtgg catgaagagc ataaaagctg gccgttaagt gaggaactgt acggcgttcc
    22501 ttcaccgtgc attatttcaa ctaccgaaga tgccgtatac tggcaacccc agccgtttac
    22561 cggggaacaa aatgtaaacg cggttgaacg cgcttttgat attgtgatac aacccacgat
    22621 tcataccttt tatacgactc agtttgccgg ggatatgcac gcgcagtttg gcgatatcaa
    22681 actgacattg ctgcaaacct ggagtgaaga cgatttccgt cgggtgcagg aaaatctgat
    22741 tggtcatctg gtgactcaga aaaggctaaa actgccgccc acgctattta tcgcgacgct
    22801 ggaagaagag ttagaggtca tctcggtgtg taatctctcc ggtgaagtgt gcaaagagac
    22861 gctaggaaca cgtaaacgga cgcatttagc ctcaaatctt gcggaattcc ttaatcaact
    22921 taagcctctt ctgtaatcca aataccccat ctggttgtga gagatctctt acagactctg
    22981 taggagatcg ccaggaaata agcgaatact taaaaagata agaatcgtta ttctcattta
    23041 aaaacaacat gttgcttgtt aattcttaac tttcatatga aattttcctt aaggcattgt
    23101 ctgtaagtgc cttgtaagac ttcgcgaaaa agacgattct atcttcgtcg acagggagtc
    23161 agacaacgaa gtgaacatca ggatgatgac acttctgaag gacacaccag gatggtgttt
    23221 cagggaaagg cttctggatg aagcgaagag gatgacgcag gacgcgttaa aggacacctc
    23281 caggatggag aatgagaacc ggtcaggatg attcggtggg tcaggaaggc caggaacact
    23341 tcaggatgaa gtatcacatc ggggtggtgt gagcaggaag caatagttca ggatgaacga
    23401 ttggccgcaa ggccagagga aaagttgtca aggatgagca gggagcaaca aaagtagccg
    23461 gaatgctgcg aaacgaaccg ggagcactgt gaatacagtg ctcccttttt ttattcctgc
    23521 tatccttcgc ggcagttttt cttattgagg ttgctttatg accactcatg accgcgttcg
    23581 tctccagttg caggcgcttg aagcgttact gcgtgaacat cagcactggc gaaacgataa
    23641 accccagccg catcaattta acagtaccca accgttcttt atggacacca tggaaccact
    23701 ggagtggttg cagtgggtgc tgatcccgcg aatgcacgat ttgttagata acaaccagcc
    23761 gttaccggga gcttttgccg ttgcacccta ttatgaaatg gcgctggcga ctgatcatcc
    23821 gcaacgcgcg cttattctgg ctgagctgga aaaactggat gctctttttg cggatgatgc
    23881 gagctaatgc tggaaatact ctatcaggat gaatggctgg ttgcggtaaa taaaccctcc
    23941 ggctggctgg ttcaccgcag ctggctggat cgcgacgaga aagtagtggt catgcaaacc
    24001 gtgcgtgacc tgataggcca gcatgtgttt actgctcatc gtctggaccg acccacttct
    24061 ggtgtgttgt tgatgggatt atccagcgag gccggacggc tgctggcaca acagtttgaa
    24121 cagcaccaaa tccagaaacg ttaccatgcg attgtgcgcg gctggttgat ggaagaagcg
    24181 gtgctggatt atccactggt ggaagaactg gacaaaatcg ctgataaatt tgcccgcgaa
    24241 gataaaggcc cgcagccagc agtgacgcat tatcgcggtc tggcgaccgt agaaatgccg
    24301 gtagcgaccg gacgttaccc gaccacgcgc tacggcctgg tggaactgga gccgaaaacc
    24361 ggacgtaagc accagctccg tcgccatctt gcccatttgc gccacccgat tattggcgac
    24421 agcaaacatg gtgatttacg ccagaatcgc agcggtgctg aacattttgg ccttcagcgg
    24481 ttaatgctgc atgccagtca gttgtcattg acgcatcctt ttactggcga gccactgact
    24541 attcacgcgg gcctggacga cacctggatg caggcgttat cacaatttgg ctggcggggt
    24601 ctgcttcctg aaaatgaaag ggttgagttc agtgcgccat cgggtcagga tggagagata
    24661 agttcctaat ccagggagaa aacggtatgg cggaaattgg tatttttgtc ggcactatgt
    24721 acggaaattc gctgttagtg gcggaagaag cggaagcaat tctgaccgcg cagggccaca
    24781 aagcaacggt atttgaagat cctgaattaa gcgactggct gccctatcag gataagtatg
    24841 ttctggtggt tacgtccacg accgggcagg gcgaccttcc tgatagcatt gtgccgctct
    24901 ttcagggaat taaagatacc ctgggcttcc agccgaatct gcgttatggc gtgattgcgc
    24961 tcggcgacag tagttatgtg aatttctgca atggtggcaa acagttcgat gccttgttgc
    25021 aggaacagag cgctcagcgg gtgggagaaa tgctgttgat tgacgccagt gagaacccgg
    25081 aaccggaaac ggaatcaaat ccgtgggtcg aacattgggg cacgctgttg tcctgatacg
    25141 ttcagcgagc ggtaaatgcc gttttagcag tgctgaatcg aatcttttca ggcaaatgcc
    25201 agtaaaaact gcttcatagt gcggattttt actggcgttt gcctggagtc aagcgatcca
    25261 tttcatactc ttctttattt cttcgtttta acccttcctt tcttgttctt gttttcattt
    25321 ccgtgaagtg gattccaccg tccagggcta atgccaaaat cgggcctcat tgaacgcatt
    25381 aatgttgtgt tgttgcacgg tgagccgcta tggcgcgctt tttatactgc tattgccaga
    25441 tataaacacg cgccgcattc ggcgaacgac ctataaaaac ggcaaaaaac accctacgtc
    25501 acctctgatt tcctggcgat gtcgcagtcc agagtgagcg tggctaacgc gaattttcag
    25561 gagtgcaaca atgagttctt taagtcaggc tgcgagcagt gtggaaaaac gcacgaatgc
    25621 tcgttactgg atagtggtga tgttgtttat cgtcacatcc ttcaactacg gcgaccgcgc
    25681 tacgctctct atcgccggtt cggaaatggc caaagatatc ggccttgatc ctgtgggaat
    25741 gggctatgtg ttctctgctt tctcatgggc ttatgttatc gggcagatcc ctggtggttg
    25801 gttgctggat cgttttggtt caaaacgcgt ctacttctgg tcgatcttta tctggtcgat
    25861 gtttaccttg ctgcaaggct tcgtcgatat ctttagtgga ttcggcatta tcgttgccct
    25921 gtttacgctg cgcttcctgg tcgggcttgc tgaagcgcca tctttccctg gcaacagccg
    25981 cattgttgcg gcctggtttc cggcgcagga aaggggaacg gcggtgtcga tttttaactc
    26041 cgcccaatac ttcgcaacgg ttatcttcgc gccgattatg ggctggttga ctcatgaagt
    26101 gggctggtca cacgtcttct tctttatggg cggtctgggc attgtcatca gctttatctg
    26161 gttgaaagtc atccacgagc caaatcaaca tccgggcgta aatcagaaag agctggagta
    26221 catcgccgcg ggtggcgcgc tgatcaatat ggatcagcaa aacaccaaag ttaaagtgcc
    26281 gttcagcgtg aagtgggggc agatcaaaca gctgctcggg tcacggatga tgatcggcgt
    26341 ttatatcggt cagtactgta tcaacgccct gacttacttt ttcattacct ggttcccggt
    26401 ttatctggtg caggcgcgcg ggatgtcgat tctgaaagcg ggctttgtgg cttccgttcc
    26461 ggcggtttgc ggttttatcg gcggtgtgct gggtgggatt atttccgact ggctgatgcg
    26521 tcgcacgggt tcactgaaca ttgcgcgtaa aacaccgatc gtgatgggca tgttgttgtc
    26581 gatggtgatg gtgttctgca actacgtcaa cgttgagtgg atgatcatcg gctttatggc
    26641 gctggccttc ttcggtaagg ggatcggtgc gctgggttgg gctgtaatgg ctgatactgc
    26701 gccaaaagag atcagcggtc tttccggtgg cctgtttaac atgtttggta acatttctgg
    26761 catcgtcacg ccaatcgcaa ttggttatat cgttggcacg actggctcct ttaatggggc
    26821 gctgatttat gttggtgttc atgccttaat cgcggtactc agctacctgg tgctggtggg
    26881 cgatatcaag cgtattgagc tgaaacctgt cgcggggcaa taagatgacg acacaatcca
    26941 gtcctgttat tactgatatg aaagtcattc cggtcgccgg acatgacagc atgttgctta
    27001 atattggtgg tgcacataac gcatatttca cccgcaatat tgtggtactc accgataatg
    27061 ccgggcatac cggcattggt gaagcgccgg gcggagaggt gatttatcaa acactggtca
    27121 aggctattcc gatggtgctg ggccaggaag ttgcgcgcct gaataaagtg gttcagcagg
    27181 tgcataaagg taatcaggca gccgattttg ataccttcgg caaaggtgcc tggacttttg
    27241 aattgcgcgt taatgccgtg gcggcgctgg aagccgcctt gcttgacctg ttaggtcagg
    27301 cgctgaatgt tccggtctgc gaactgttag ggccaggcaa gcaacgtgat gctattaccg
    27361 ttctcggtta tctgttttat atcggtgatc ggaccaaaac cgatcttcct tatctggaaa
    27421 atacgtcggg caaccatgag tggtatcagt tgcggcatca gaaagcgatg aacagcgaag
    27481 ccgttgtgcg tctggcggaa gcctcacagg atcgctatgg ctttaaagat ttcaaactta
    27541 agggtggcgt gttacctggc gagcaagaaa tcgacactgt tcgtgcattg aagaaacgct
    27601 tccctgatgc gcggattacc gttgatccca atggtgcctg gctgcttgat gaagccattt
    27661 ctctgtgcaa agggctgaat gatgtcctta cctatgcgga agacccgtgc ggcgctgagc
    27721 agggtttctc cggtcgtgaa gtgatggcgg agtttcgacg ggcgaccggc ttgcccgtcg
    27781 cgaccaacat gatcgccacc aactggcgcg aaatgggtca tgcggtgatg ctcaatgcgg
    27841 tagatattcc acttgccgat ccgcacttct ggacgctttc cggtgcagtc cgcgtggcgc
    27901 aactttgcga cgactggggg ctgacctggg gctgccattc taataaccac tttgatatct
    27961 ctctggcgat gtttacccat gtgggcgcgg cggcaccggg taatcctacc gctatcgata
    28021 cccactggat ttggcaggag ggcgattgtc gcctgaccaa aaatccgctg gagattaaaa
    28081 acggaaaaat tgccgttcct gatgcgcccg gtctgggcgt ggaactggac tgggagcagg
    28141 tacaaaaggc gcatgaggcc tataaacgtc tgcctggcgg tgcgcgtaac gacgcgggcc
    28201 cgatgcagta cctgatcccc ggctggacct ttgaccgtaa acgtcccgtt ttcggccgtc
    28261 attgattttg aaaaaggaca taaatatgag ttctcaattt acgacgcctg ttgttactga
    28321 aatgcaggtc atcccggtgg cgggccatga cagtatgctg atgaatctga gtggtgcaca
    28381 cgcaccgttc ttcacgcgta atattgtgat tatcaaagat aattctggtc acactggggt
    28441 aggggaaatt cccggcggcg agaaaatccg taaaacgctg gaagatgcga ttccgctggt
    28501 ggtgggtaaa acgctgggtg aatacaaaaa cgttctgacg ctggtgcgta atacatttgc
    28561 cgatcgtgat gccggcgggc gcggtttgca gacatttgat ttgcggacca ctattcatgt
    28621 agttaccggg atagaagcgg caatgctgga tctgctgggg cagcatctgg gggttaacgt
    28681 ggcatcgctg ctgggcgatg gtcaacagcg tagcgaagtc gaaatgctcg gttatctgtt
    28741 cttcgtcggt aatcgcaaag ccacaccgct gccgtatcaa agccagccgg atgataaatg
    28801 tgactggtat cgcctgcgtc acgaagaagc gatgacgccg gatgcggtgg tgcgcctggc
    28861 ggaagcggca tatgaaaaat atggcttcaa cgatttcaaa ctgaagggtg gtgtactggc
    28921 cggggaagaa gaagcagaat ctattgtggc actggcgcaa cgcttcccac aggcgcgtat
    28981 taccctcgat cctaacggtg cctggtcgct gaacgaagcg attaaaattg gtaaatacct
    29041 gaaaggttcg ctggcttatg cagaagatcc gtgtggtgcg gagcaaggtt tctccgggcg
    29101 tgaagtgatg gcagagttcc gtcgcgcgac tggcctgccg accgcaacca atatgatcgc
    29161 caccgactgg cggcagatgg gccatacgct ctcgttacaa tccgttgata tcccgctggc
    29221 ggatccgcat ttctggacga tgcaaggttc ggtacgtgtg gcgcaaatgt gccatgaatt
    29281 tggcctgacc tggggttcac actctaacaa ccacttcgat atttccctgg cgatgtttac
    29341 ccatgtagcc gctgctgcac cgggtaaaat tactgctatt gatacgcact ggatctggca
    29401 ggaaggcaat cagcgtctga ccaaagaacc gttagagatc aaaggcggtc tggttcaggt
    29461 gccagaaaaa ccggggctgg gtgtagaaat cgatatggat caagtgatga aagcgcacga
    29521 gctgtatcag aaacacgggc ttggcgcgcg tgatgatgcg atgggaatgc agtacctgat
    29581 ccccggctgg acgttcgata acaagcgtcc gtgcatggtg cgttaagaca gaaaaccaaa
    29641 accggctcta tctggagccg gtttgagact gatgccaaac gcaaaattgc ctgttgcgct
    29701 acgtatcagg gctacatcat tttttaattc attgaattta attggttttg caggccggat
    29761 aaggcgttaa cgccgcatcc ggcataaaca caggcacttg tcaccaatct gaaaccagcg
    29821 tcataaaaat ccggttgcta ctcgacaaga cgtccattac ccgagaattt tgctggcttc
    29881 gcgcgctaca ttatccatct cgtccaacag ttccagcagt tccggctcca aatcttcttc
    29941 tttagtaccg ctacgtaact gttgttcgat cagttggcag agattcttca tacgcggcac
    30001 accgctatag ccgcaactgc catgcagttt atgaatcaaa tcaaccaggc cttccgggtt
    30061 ttctccaacc agctgttcct caactttgtt gcgaacttca ggcaggaaat cgagtaacat
    30121 ttgcagcata tcgcgcgcta aatcggtttt tcctgctgcc tggcgtagtg ccagttgcca
    30181 gtcgagggtc gcattcgggt tcaccacaat ttcgttgact tcgggcgtca cgacgcgaga
    30241 ggaaataccg ctgccaggct tgtagcgcaa caacaaatta tgcaatcgct cttcttcaat
    30301 cggtttcgcc agataatcgc tcatccctgc gccaagcagc ttctcttttt gcccggccat
    30361 tgcatgcgcc gttaccgcga taaccggcgt ttgttgctga tgggggagct ggtggatgag
    30421 ttcgcaggcc cgaatgccgt ccatgtccgg catttgaata tccattaaga tcaaatcgaa
    30481 cggcatctgt ttcgcccgtt caaccgcctg atgcccgcta tcgcaaagtt ccacatgttg
    30541 caccatatct tccagcaatg cgccgataag tttcaggtta gcggggttgt catcaaccgc
    30601 catgactgtc attgccagct tactttcatc ggttacaggc aaaagcgtgt tttgtttgtg
    30661 atgacaaaat tccgtcaggg caggcaacag gcgcgtaggt gttaatggtt tcagcagaca
    30721 cgcgccgata ccatcttgct tgagtttttc agcattgact tgtgcatggc aaggaagtgc
    30781 cagcatcagg aaatcggtca tcgataccgc tttcgctaat cgctcatgtt gcattgttag
    30841 cggctcgcgg aaggtcaccg cgatgcctaa caacatcatg tcgtaatgcg cgggaggcag
    30901 cgcggagaac gttgggctat aaaccacctc cagcggcgtt tcacttaaaa tatccagcgt
    30961 gcattgcgct gctgcggagt ttggttcgac ataggccagg cgcttgccgg cgaggcactg
    31021 ggtggatggc ccttcaataa taatgttcgg attcagatcg agattaatgt ggaaccagaa
    31081 agttgaaccg cgattcggct ggctatgaaa cgaaatatcg ccgcccattt cattaaccag
    31141 tttttgtgta atcaccagcc ccagcccggt gccaccatga cggcgggaaa tactggcatc
    31201 agcctgtcgg aaggcctgga ataagcgcga ttgatcgcgt tcaggaatac caatgccggt
    31261 atcccgaatc tgcacttcaa tctgcacttt ggtattactc agcgcacgtt tttctaccag
    31321 aatatcaatg ttgccattct cggtgaattt aattgcattc cccaccaggt tagtgatgat
    31381 ttgctgtaat cgtagtgggt cgccgataac gttatcaggc acgtcgcttt taatattgag
    31441 cgtcagttcc agtcctttat cgtgagaaga atgtgccagc agggtaacga cttcatccag
    31501 cgtgctgcgt agtgggaatg gaatactttc cagaatcagc ttacctgctt ccagtttcga
    31561 gaagtcgaga acatcattaa taattgccag caaattattt gccgaacgtt caatcgtatt
    31621 caggtgatcg cgctgcgttg gtgttaattc tgttttcagc gtcaggcggg taaagccaat
    31681 aacaccattc agtggtgtac gcagctcgtg tgacatattt gccagaaact cggatttaat
    31741 acgcgccgct tcctgggcgc gctttttcgc cagatccaac tcaacgttct gaatttccat
    31801 ctgctccagc gtttcacgca gatcggacgt cgcctggtcg atattgtgct gcatctcttc
    31861 gtgataagca gccagcgaca ttgccatcga gttgataccg tttttcagca tatccagctc
    31921 gccgagcata aatccttcca cccggctgtc gagttgcccg cgacggatgc ggtcgacagt
    31981 attcaccatg ttgcgaatcg gaccggttac atcgcgcatt aagcgccagc caaaaataag
    32041 cgcaataccg atacaaaaca gcatcatcac gctggaaata aagatctctt tatattgctg
    32101 caagcgaacc gatttaagat ccagctccag cgcaatatat cccagcatat tttgactatt
    32161 tttggcatcg ctactgggcg attcgtcggg ggagtaactt tcagaaataa tcggcgtgcg
    32221 gaggatcatt atatcgccgt cacgagtgac agtgagctgg cgaggaaacg gcacgttgct
    32281 gccgagctgc attgatgagg gatcaagatg aaaattggag gtgacaaaga gtcggttatt
    32341 ttcatcataa accgaaatcg cgcgaacaat atcggaatgg cgacgatgca gtacgcttat
    32401 taactgaccg atagattcgc gattttgcag gctcatgcca tattcagtag aaactgcaag
    32461 cggctcaata atgctggctc cggcatcttc cagttgacgc tgcaagtcgt tatagcgatg
    32521 cacaacgaaa aagatactca gcaataaacc aataaggacg gtcggtgcca ggatcagaat
    32581 catcatgcgt gcgcgcaggc tgtagttggt catggagttc cgttatggga caattaaggt
    32641 cacactgtta aattgagaaa gtctcgccaa tggcgcaatt ctactctgca aaacgacgca
    32701 cgacgacgcg tcagatcata actgtttcag tcaacgacct cgactctttt ggtcagggcg
    32761 tggcacgaca taacggcaaa gcgttattta ttcctggatt attgccacag gaaaacgcgg
    32821 aagttactgt tactgaagat aaaaaacaat acgcccgcgc taaagtcgtg cgccggttaa
    32881 gcgatagccc ggaacgcgaa acgccacgct gtcctcattt tggcgtatgc ggcggctgtc
    32941 agcaacaaca cgccagcgtg gatttacagc agcgaagcaa aagtgcggca ctcgcccgat
    33001 taatgaaaca cgaagtctct gaagtgatcg ccgatgttcc ctggggctat cgccgtcgcg
    33061 cgcgtttaag tctgaactac ttaccgaaaa cacagcaact tcagatgggg tttcgcaaag
    33121 cgggctccag tgacattgtc gacgtgaaac aatgccccat tttagtgccc caacttgaag
    33181 cattgctgcc caaagtcagg gcatgcctgg gcagcttaca agctatacgc catcttggtc
    33241 atgttgaact ggtacaggca accagcggca cgctgatgat tttgcgccat accgcaccgc
    33301 taagttcggc tgatcgcgaa aaactggaac gcttttcgca ttctgaaggc ctggatctgt
    33361 atctcgcccc cgatagtgag atactcgaaa ccgtctctgg tgagatgccc tggtatgact
    33421 caaacgggtt gcgcttaact tttagcccgc gcgattttat tcaggtcaat gcgggtgtga
    33481 accaaaaaat ggtagcgcgt gcgttggaat ggctggatgt acaacctgaa gatcgcgtac
    33541 tggatttgtt ctgcggtatg ggcaacttta cactgccatt ggcgacacaa gctgccagtg
    33601 tggtgggtgt agaaggcgtt ccggcgctgg tggaaaaagg ccagcagaat gcgcgtctta
    33661 atggcttaca gaatgtgacg ttttatcacg aaaatcttga agaagatgtc acaaagcagc
    33721 cgtgggcgaa aaacggcttc gataaagtgt tgttggaccc ggcgcgagca ggtgccgcag
    33781 gtgttatgca gcaaattata aaactggaac ctattcgtat agtttatgta tcctgtaacc
    33841 ctgcaacgct ggctcgggat agcgaagcgt tattaaaagc aggatatacc attgcgcgac
    33901 tggcgatgct ggatatgttc ccacacacgg gacatctgga atcgatggta cttttctcgc
    33961 gcgttaaata gttacgattt gccgatttcg gcaggtctgg tccctaaagg agaggacgat
    34021 ggttgcggta agaagtgcac atatcaataa ggctggtgaa tttgatccgg aaaaatggat
    34081 cgcaagtctg ggtattacca gccagaagtc gtgtgagtgc ttagccgaaa cctgggcgta
    34141 ttgtctgcaa cagacgcagg ggcatccgga tgccagtctg ttattgtggc gtggtgttga
    34201 gatggtggag atcctctcga cattaagtat ggacattgac acgctgcggg cggcgctgct
    34261 gttccctctg gccgatgcca acgtagtcag cgaagatgtg ctgcgtgaga gcgtcggtaa
    34321 gtcggtcgtt aaccttattc acggcgtgcg tgatatggcg gcgatccgcc agctgaaagc
    34381 gacgcatacc gattctgttt cctccgaaca ggtcgataac gttcgccgga tgttattggc
    34441 gatggtcgat gattttcgct gcgtggtcat caaactggca gagcgtattg ctcatctgcg
    34501 cgaagtaaaa gacgcgccgg aagatgaacg cgtactggcg gcaaaagagt gcaccaatat
    34561 ctacgcgccg ttggcgaacc gtcttgggat tgggcaactg aaatgggagc tggaagatta
    34621 ctgcttccgt tatctccacc cgaccgaata caaacgcatc gcaaaactgc tgcatgaacg
    34681 ccgtctcgac cgcgaacact atatcgaaga gtttgtcggc catctgcgcg ctgagatgaa
    34741 agctgaaggc gttaaagctg aagtgtatgg ccgtccgaaa cacatctaca gtatctggcg
    34801 caaaatgcag aaaaagaacc tcgccttcga tgagctgttt gatgtgcgtg cggtacgtat
    34861 tgtcgccgag cgtttacagg attgttatgc cgcactgggg atagtgcaca ctcactatcg
    34921 ccacctgccg gatgagttcg acgattacgt cgctaacccg aaaccaaacg gttatcagtc
    34981 tattcatacc gtggttctgg ggccgggtgg caaaaccgtt gagatccaaa tccgcaccaa
    35041 acagatgcat gaagatgcag agttgggtgt tgctgcgcac tggaaatata aagagggcgc
    35101 ggcagctggc ggcgcacgtt cgggacatga agaccgaatt gcctggctgc gtaaactgat
    35161 tgcgtggcag gaagagatgg ctgattccgg cgaaatgctc gacgaagtac gtagccaggt
    35221 ctttgacgac cgggtgtacg tctttacgcc gaaaggtgat gtcgttgatt tacctgcggg
    35281 atcaacaccg ctggacttcg cttaccacat ccacagtgat gtcggacacc gctgcatcgg
    35341 ggcaaaaatt ggcgggcgca ttgtgccgtt cacctaccag ctgcagatgg gcgaccagat
    35401 tgaaattatc acccagaaac agccgaaccc cagccgtgac tggttaaacc caaacctcgg
    35461 ttacgtcaca accagccgtg ggcgttcgaa aattcacgcc tggttccgta aacaggaccg
    35521 tgacaaaaac attctggctg ggcggcaaat ccttgacgac gagctggaac atctggggat
    35581 cagcctgaaa gaagcagaaa aacatctgct gccgcgttac aacttcaatg atgtcgacga
    35641 gttgctggcg gcaattggtg gcggggatat ccgtctcaat cagatggtga acttcctgca
    35701 atcgcaattt aataagccga gtgccgaaga gcaggatgcc gccgcgctga agcaacttca
    35761 gcaaaaaagc tatacgccgc aaaaccgcag taaagataac ggtcgtgtgg tggtagaagg
    35821 tgtcggtaac ctgatgcacc acatcgcgcg ctgctgccag ccgattcctg gagatgagat
    35881 tgtcggcttc attacccagg ggcgcggtat ttcagtacat cgcgccgatt gtgaacaact
    35941 ggcggaactg cgctcccatg cgcccgaacg cattgttgac gcggtatggg gcgagagcta
    36001 ctccgccgga tattcgctgg tggtccgcgt ggtggctaat gatcgtagtg ggttgttacg
    36061 tgatatcacg accattctcg ccaacgagaa ggtgaacgtg cttggcgttg ccagccgtag
    36121 cgacaccaaa cagcaactgg cgaccatcga catgaccatt gagatttaca acctgcaagt
    36181 gctggggcgc gtgctgggta aactcaacca ggtgccggat gttatcgacg cgcgtcggtt
    36241 gcacgggagt tagtcttaca tagctggata tggcgtcagc cgtatccagc gatatctttt
    36301 actcgaaggt attacacctg acggacccgc aattttcagg acattctaat gaatcaaatc
    36361 gaccgtttgc tcaccatcat gcagcgcctg cgcgacccgg aaaacggctg cccgtgggat
    36421 aaagagcaga catttgccac cattgcgcct tacacccttg aagaaaccta cgaggtgctg
    36481 gacgccatcg cccgtgaaga ttttgacgat ctgcgcggtg aactgggcga tctgctgttc
    36541 caggtggtgt tttacgcaca aatggcccag gaagaagggc gctttgattt taatgatatt
    36601 tgcgctgcga ttagcgataa attagaacgc cgtcatccac atgtttttgc tgacggttct
    36661 gccggaaaca gccgcgaagt tcttgctcgt tgggagcaaa tcaaaactga agagcgcgcg
    36721 caaaaagcgc agcattcggc gctggacgat atccctagta gtttacccgc cttaatgcgc
    36781 gcacaaaaaa tccagaaacg ttgcgccaac gttggcttcg actggacgac gcttggcccg
    36841 gtagtcgata aagtctacga agagatcgat gaggtgatgt acgaagcgcg gcaggttgtt
    36901 gtcgaccagg ctaaactgga ggaggaaatg ggtgacctgc tgtttgccac agtcaatctg
    36961 gctcgtcatt tagggacaaa agcggaaatc gcattgcaaa aagcgaacga aaaattcgag
    37021 cgtcgttttc gcgaagtgga gcgtattgtt gccgcgcgtg gactggaaat gacaggtgtt
    37081 gacctcgaaa caatggaaga agtctggcaa caggtaaaac ggcaggaaat tgatctctaa
    37141 gggaattacg tggtcaagcg cgatttgtgt cattttttaa atgacaagcg cttgatttgc
    37201 gtcaaaaaca tttaccccaa aggggctatt ttctcactcc tgatttcaat agtgcgctgg
    37261 cgaagaggag ggataatgaa agtttgtggc acaggtcatg ttcgggtata ctgctttccc
    37321 gtcctggtta ttccatcgtc ttttcaacct aacttctcag gttcagcatg acaacgaact
    37381 atatttttgt gaccggcggg gtcgtatcct ctctgggtaa aggcattgcc gcagcctccc
    37441 tcgcagccat tcttgaagcc cgtggcctca atgtgaccat catgaaactg gatccgtaca
    37501 tcaacgtcga tccaggtact atgagcccaa tccaacacgg ggaagtgttc gttactgaag
    37561 acggcgctga aaccgacctg gacctggggc actacgagcg tttcattcgt accaaaatga
    37621 gccgccgcaa caacttcacc acgggtcgta tctactctga cgttctgcgt aaagaacgcc
    37681 gcggtgacta cctcggcgca accgtgcagg ttattccgca catcactaac gcaatcaaag
    37741 agcgcgtgct ggaaggcggc gaaggtcatg acgtagtact ggtcgaaatc ggcggtactg
    37801 taggtgatat cgaatccctg ccgttcctcg aagcgattcg ccagatggct gttgaaattg
    37861 gccgtgagca cactctgttt atgcacctga cgctggtgcc gtacatggca gcgtctggtg
    37921 aagtcaaaac caaaccgact cagcactctg taaaagagct gctctccatc ggtatccagc
    37981 ctgacatcct gatttgtcgt tcagatcgcg ccgttccggc gaacgaacgt gcgaagattg
    38041 cattgttctg taatgttccg gaaaaagcgg ttatttctct gaaagacgtc gattccatct
    38101 ataaaattcc gggcctgttg aaatctcagg ggctggacga ttatatttgt aaacgattca
    38161 gcttaaactg cccggaagca aatctgtccg aatgggaaca ggttatcttc gaagaagcga
    38221 atccggtaag tgaagtcacc atcggtatgg tcggcaagta cattgaactg ccggatgctt
    38281 ataaatcagt gatcgaagca ctgaaacacg gtgggctgaa gaatcgtgtc agcgtcaaca
    38341 tcaaactgat cgattcacaa gatgttgaaa cgcgcggcgt tgaaatcctt aaaggtctgg
    38401 acgcaatcct cgtacctggc ggtttcggct atcgtggcgt agaaggcatg attacgaccg
    38461 cgcgttttgc gcgtgagaac aatattcctt atctgggtat ttgcctgggt atgcaggtgg
    38521 cgttaattga ttacgctcgc catgttgcca acatggagaa cgccaactct acggaatttg
    38581 tgccagactg taagtacccg gttgtggcgc tgattaccga gtggcgcgat gaaaacggca
    38641 acgttgaagt tcgtagcgag aagagcgatc tcggcggtac catgcgtctc ggcgcacagc
    38701 agtgccagtt ggttgacgat agcctggttc gccagctgta caatgcgccg acaattgttg
    38761 agcgtcatcg tcaccgttac gaagtcaaca acatgctgtt gaaacagatt gaagatgcag
    38821 gtctgcgcgt tgcgggccgt tccggggatg atcagttggt cgagatcatc gaagttccga
    38881 atcacccgtg gttcgtggct tgccagttcc atccggagtt tacttctact ccacgtgatg
    38941 gtcacccgct gtttgcaggc tttgtgaaag ccgccagcga gttccagaag cgtcaggcga
    39001 agtaagaaaa aagttagaac ggcaacgcgt accctaggta cgcgttgttt gtctggagtt
    39061 tcagtttaac tagtgacttg aggaaaacct aatgtccaaa atcgtaaaaa tcatcggtcg
    39121 tgaaatcatc gactcccgtg gtaacccgac tgttgaagcc gaagtacatc tggagggtgg
    39181 tttcgtcggt atggcagctg ctccgtcagg tgcttctact ggttcccgtg aagctctgga
    39241 actgcgcgat ggcgacaaat cccgtttcct gggtaaaggc gtaaccaaag ctgttgctgc
    39301 ggtaaacggc ccgatcgctc aggcgctgat tggcaaagat gctaaagatc aggctggcat
    39361 tgacaagatc atgatcgacc tggacggcac cgaaaacaaa tccaaattcg gcgcgaacgc
    39421 aatcctggct gtatctctgg ctaacgccaa agctgctgca gctgctaaag gtatgccgct
    39481 gtacgagcac atcgctgaac tgaacggcac tccgggcaaa tactccatgc cggttccgat
    39541 gatgaacatc atcaacggtg gtgagcacgc tgacaacaac gttgatatcc aggaattcat
    39601 gattcagccg gttggcgcga aaactgtgaa agaagccatc cgcatgggtt ctgaagtttt
    39661 ccatcacctg gcaaaagttc tgaaagcgaa aggcatgaac actgctgttg gtgacgaagg
    39721 tggctatgcg ccgaacctgg gttccaacgc tgaagctctg gctgttatcg ctgaagctgt
    39781 taaagctgct ggttatgaac tgggcaaaga catcactctg gcgatggact gcgcagcttc
    39841 tgaattctac aaagacggta aatacgttct ggctggcgaa ggcaacaaag cgttcacctc
    39901 tgaagaattc actcacttcc tggaagaact gaccaaacag tatccgatcg tttccatcga
    39961 agacggtctg gacgaatctg actgggacgg tttcgcatac cagaccaaag ttctgggcga
    40021 caaaatccag ctggttggtg acgacctgtt cgtaaccaac accaagatcc tgaaagaagg
    40081 tatcgaaaaa ggtatcgcta actccatcct gataaaattc aaccagatcg gttctctgac
    40141 cgaaactctg gctgcaatca agatggcgaa agacgctggc tacactgcag ttatctctca
    40201 ccgttcaggc gaaactgaag acgctaccat cgctgacctg gctgttggta ctgctgcagg
    40261 ccagatcaaa actggttcta tgagccgttc tgaccgtgtt gctaaataca accagctgat
    40321 tcgtatcgaa gaagctctgg gcgaaaaagc accgtacaac ggtcgtaaag agatcaaagg
    40381 ccaggcataa gtctgacttt atctgattta aaaatgccag ccttcgggct ggcgtttttt
    40441 atggttgagt taccagcgac cggacgcgcc gccaccgcca gaagaaccgc ccccgccgct
    40501 aaatccgccg ccacttgagc cgccaccgga accgctgttg tctgaatgat aacttctacc
    40561 agatgctttt tttctgaagt acacataaac aggtaaagac cataatggtg gtgaaagtaa
    40621 aaaagaagaa aaatacagca atggaaaaag ttttatcgga gaacaggaat aaatctaaaa
    40681 gatagattgc tgaaatacag acactgcttt tacatgcgcg acaaaaatta ctgccttgat
    40741 gaaaaaacca gaaagggaag aacatcactg caaataccca aaaaatagca aagtagtaat
    40801 gatcgctgga agacgctgat tcactttctg aaggattagc cgaatattgg tgttgtgaag
    40861 tgagttggtt attcaaagcg attatagcta actctaatcc cttagctaat ttttgttgtt
    40921 taaatgcggg tatcatgttg ctacggatga tatctccagc cagagcatcg gttacctttt
    40981 cttccagccc ataacctacc tggatgcgga cagtgcgatc cgaccaggca acaatgatca
    41041 gtatcccatc attgcgtttg gcatctccta aacgccaatt gttaaaaacc cttgtagcat
    41101 attgttcaat tgtctcgtct ttggttgtgg gaataattaa tacagcaatt tgtacttggg
    41161 tattattgaa aatagattct aacttccgcg tcagttcttt cttttcttgt gagtttaagc
    41221 tactcgtaag atccgtaact tgttgctgta attgtgggac aatagttggt tgggctgcaa
    41281 caaaggaaca cacgaatgtg aatattagta tgaaatagcg cattataaat gtcccattaa
    41341 agctggataa ataaattgac ggcggcatcg gtaatttgag attc
//
LOCUS       DAPHCV010000031        40494 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.031, whole genome shotgun sequence.
ACCESSION   DAPHCV010000031 DAPHCV010000000
VERSION     DAPHCV010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.031"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1731
                     /gene="adeD"
                     /locus_tag="RH033_001677"
     CDS             <1..1731
                     /gene="adeD"
                     /locus_tag="RH033_001677"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDV2990873.1"
                     /translation="SRAEYQELLAVSRGDAVADYIIDNVSILDLINGGEISGPIVIKG
                     RYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLTT
                     VICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQML
                     AWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYIAAG
                     IENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDRNP
                     WEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVLL
                     SDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIDRQPVSASDFALQFTP
                     GKRYRVIEVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRLAPACGLLGGFGL
                     NEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPIAG
                     LMSTDTAQSLAEQIDTLKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDGEK
                     FAFTTLEVTE"
     gene            complement(1778..3169)
                     /gene="uhpT"
                     /locus_tag="RH033_001678"
     CDS             complement(1778..3169)
                     /gene="uhpT"
                     /locus_tag="RH033_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HDV2990874.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3425..4744)
                     /gene="uhpC"
                     /locus_tag="RH033_001679"
     CDS             complement(3425..4744)
                     /gene="uhpC"
                     /locus_tag="RH033_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HDV2990875.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGVSALLLLPFLNAQTPREA"
     gene            complement(4754..6256)
                     /gene="uhpB"
                     /locus_tag="RH033_001680"
     CDS             complement(4754..6256)
                     /gene="uhpB"
                     /locus_tag="RH033_001680"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HDV2990876.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFFLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHSVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6256..6846)
                     /gene="uhpA"
                     /locus_tag="RH033_001681"
     CDS             complement(6256..6846)
                     /gene="uhpA"
                     /locus_tag="RH033_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HDV2990877.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6919..7209)
                     /gene="ilvN"
                     /locus_tag="RH033_001682"
     CDS             complement(6919..7209)
                     /gene="ilvN"
                     /locus_tag="RH033_001682"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDV2990878.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7213..8901)
                     /gene="ilvB"
                     /locus_tag="RH033_001683"
     CDS             complement(7213..8901)
                     /gene="ilvB"
                     /locus_tag="RH033_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HDV2990879.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(9007..9105)
                     /gene="ivbL"
                     /locus_tag="RH033_001684"
     CDS             complement(9007..9105)
                     /gene="ivbL"
                     /locus_tag="RH033_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HDV2990880.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9669..9758
                     /gene="tisB"
                     /locus_tag="RH033_001685"
     CDS             9669..9758
                     /gene="tisB"
                     /locus_tag="RH033_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HDV2990881.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            10038..11222
                     /gene="emrD"
                     /locus_tag="RH033_001686"
     CDS             10038..11222
                     /gene="emrD"
                     /locus_tag="RH033_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HDV2990882.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNGGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLIPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11230..11727)
                     /locus_tag="RH033_001687"
     CDS             complement(11230..11727)
                     /locus_tag="RH033_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDV2990883.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQLGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDTTLQKWCGSEKS
                     QPRRDCQACDWHRLCPHAREDKPDNVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11724..12086)
                     /locus_tag="RH033_001688"
     CDS             complement(11724..12086)
                     /locus_tag="RH033_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HDV2990884.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12076..12423)
                     /locus_tag="RH033_001689"
     CDS             complement(12076..12423)
                     /locus_tag="RH033_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HDV2990885.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            complement(12483..13976)
                     /locus_tag="RH033_001690"
     CDS             complement(12483..13976)
                     /locus_tag="RH033_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDV2990886.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAESIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARADEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYELGEKS
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGDNILAVKEPRGVLVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIHFADVRSKMHDALLDYMDKIRDPFRSYQWNLR
                     PWRKDAQPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(13973..15688)
                     /locus_tag="RH033_001691"
     CDS             complement(13973..15688)
                     /locus_tag="RH033_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HDV2990887.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQLSDG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     MGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            15855..16721
                     /locus_tag="RH033_001692"
     CDS             15855..16721
                     /locus_tag="RH033_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDV2990888.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNDEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEVYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLNARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDR"
     gene            complement(16811..18472)
                     /locus_tag="RH033_001693"
     CDS             complement(16811..18472)
                     /locus_tag="RH033_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HDV2990889.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGVGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMAMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESTRTNGGALIRTINIRVENPNLHN
                     LAIKDVPILNGDKVICSRLKREETLKVPSPETVIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASSD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFIHTLVDGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(18668..19096)
                     /gene="ibpB"
                     /locus_tag="RH033_001694"
     CDS             complement(18668..19096)
                     /gene="ibpB"
                     /locus_tag="RH033_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HDV2990890.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(19208..19621)
                     /gene="ibpA"
                     /locus_tag="RH033_001695"
     CDS             complement(19208..19621)
                     /gene="ibpA"
                     /locus_tag="RH033_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HDV2990891.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(19615..19687)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            19927..20259
                     /locus_tag="RH033_001696"
     CDS             19927..20259
                     /locus_tag="RH033_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDV2990892.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVLDTILLPWDVFRKDSSVRSRVEKSEADAQETNNVI
                     PPAKMPAN"
     gene            complement(20263..21477)
                     /locus_tag="RH033_001697"
     CDS             complement(20263..21477)
                     /locus_tag="RH033_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HDV2990893.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAYVGVVTVHPKSEKYVFIHGPENPDETWYYDFHHRRGVIVE
                     SGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSAKGEKVPELFIVELPKDEAGWKAAGDVPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EDGQLWITETDR"
     gene            21578..22678
                     /gene="cbrA"
                     /locus_tag="RH033_001698"
     CDS             21578..22678
                     /gene="cbrA"
                     /locus_tag="RH033_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HDV2990894.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQYGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKLNTAYRRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(22639..23976)
                     /locus_tag="RH033_001699"
     CDS             complement(22639..23976)
                     /locus_tag="RH033_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2990895.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFIGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(24051..25199)
                     /gene="dgoD"
                     /locus_tag="RH033_001700"
     CDS             complement(24051..25199)
                     /gene="dgoD"
                     /locus_tag="RH033_001700"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HDV2990896.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(25196..25813)
                     /gene="dgoA"
                     /locus_tag="RH033_001701"
     CDS             complement(25196..25813)
                     /gene="dgoA"
                     /locus_tag="RH033_001701"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HDV2990897.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEKSIPAIVDAYGDKALIGAGTVLKPEQVDALARMSCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALDAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(25797..26675)
                     /gene="dgoK"
                     /locus_tag="RH033_001702"
     CDS             complement(25797..26675)
                     /gene="dgoK"
                     /locus_tag="RH033_001702"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HDV2990898.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLDSRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGDQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGFNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(26672..27361)
                     /gene="dgoR"
                     /locus_tag="RH033_001703"
     CDS             complement(26672..27361)
                     /gene="dgoR"
                     /locus_tag="RH033_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HDV2990899.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            27638..28294
                     /locus_tag="RH033_001704"
     CDS             27638..28294
                     /locus_tag="RH033_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2990900.1"
                     /translation="MKLNFKGFFKAAGLCSLAFALTGCITWGLVSNTASRPHAEQWRS
                     DTIKGLSLAEDSNGTKGYVFVGESLDYLLTTGGDEVVKMLNDPAIHGERITVSDNAKF
                     ILSSSNKNFSGAITLYYDWNNEEDKALATQYGFICDTRRCTWMLDGLKGSIHQKNKKA
                     DYSNVMVFHQPFTVGFYEYKATDGVPRGLVNALLPVTLTLDIVTSPLQFLILCTTRNC
                     "
     gene            complement(28338..29150)
                     /gene="yidA"
                     /locus_tag="RH033_001705"
     CDS             complement(28338..29150)
                     /gene="yidA"
                     /locus_tag="RH033_001705"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDV2990901.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(29265..29663)
                     /locus_tag="RH033_001706"
     CDS             complement(29265..29663)
                     /locus_tag="RH033_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HDV2990902.1"
                     /translation="MGLFDDVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQPVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(29903..32317)
                     /gene="gyrB"
                     /locus_tag="RH033_001707"
     CDS             complement(29903..32317)
                     /gene="gyrB"
                     /locus_tag="RH033_001707"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDV2990903.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHNIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(32346..33419)
                     /gene="recF"
                     /locus_tag="RH033_001708"
     CDS             complement(32346..33419)
                     /gene="recF"
                     /locus_tag="RH033_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDV2990904.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLIMPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(33419..34519)
                     /gene="dnaN"
                     /locus_tag="RH033_001709"
     CDS             complement(33419..34519)
                     /gene="dnaN"
                     /locus_tag="RH033_001709"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDV2990905.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(34524..35927)
                     /gene="dnaA"
                     /locus_tag="RH033_001710"
     CDS             complement(34524..35927)
                     /gene="dnaA"
                     /locus_tag="RH033_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDV2990906.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            36224..36304
                     /locus_tag="RH033_001711"
     CDS             36224..36304
                     /locus_tag="RH033_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2990907.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTRQPV"
     gene            36534..36674
                     /gene="rpmH"
                     /locus_tag="RH033_001712"
     CDS             36534..36674
                     /gene="rpmH"
                     /locus_tag="RH033_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDV2990908.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            36691..37050
                     /gene="rnpA"
                     /locus_tag="RH033_001713"
     CDS             36691..37050
                     /gene="rnpA"
                     /locus_tag="RH033_001713"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDV2990909.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            37014..37271
                     /gene="yidD"
                     /locus_tag="RH033_001714"
     CDS             37014..37271
                     /gene="yidD"
                     /locus_tag="RH033_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDV2990910.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            37274..38920
                     /gene="yidC"
                     /locus_tag="RH033_001715"
     CDS             37274..38920
                     /gene="yidC"
                     /locus_tag="RH033_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDV2990911.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            39026..40390
                     /gene="mnmE"
                     /locus_tag="RH033_001716"
     CDS             39026..40390
                     /gene="mnmE"
                     /locus_tag="RH033_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDV2990912.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         9750 a        11032 c        10401 g         9311 t
ORIGIN      
        1 agccgggctg aataccagga attgttagcc gtttcccgtg gagacgctgt tgccgattat
       61 attattgata atgtctctat tctcgacctg atcaatggcg gagaaatttc cggcccaatt
      121 gtgattaaag gacgttacat tgccggtgtt ggcgcagaat acgctgatgc tccggctttg
      181 cagcggattg atgcccgcgg cgcaacggcg gtgccagggt ttattgatgc tcacctgcat
      241 attgaatcca gcatgatgac gccggtcacc tttgaaactg ctaccctgcc gcgcggcctg
      301 acgaccgtta tttgcgaccc tcatgaaatc gtcaacgtga tgggcgaagc cggtttcgcc
      361 tggtttgccc gctgtgccga acaggcaagg caaaaccaat acttgcaggt cagctcttgc
      421 gtacccgccc tggaaggctg cgatgttaac ggtgccagtt ttacccttga acagatgctc
      481 gcctggcggg accatccgca ggttaccggc cttgcagaaa tgatggacta ccctggcgta
      541 attagcgggc agaatgcact gctcgataaa ctggatgcat ttcgccacct gacgctggac
      601 ggtcactgcc cgggtttggg tggtaaagaa cttaacgcct atattgctgc gggtattgaa
      661 aactgccacg aaagttatca gctggaagaa ggacgccgga aattacaact cggcatgtcg
      721 ttgatgatcc gcgaagggtc cgctgcccgc aatctcaacg cgctggcacc gttgatcaac
      781 gaatttaaca gcccgcaatg catgctctgt accgatgacc gtaacccgtg ggaaatcgcc
      841 catgaaggac acatcgatgc cttaattcgc cgcctgatcg aacaacacaa tgtaccgctg
      901 catgtggcat atcgcgtagc cagctggtcg acggcgcgcc attttggtct gaatcacctc
      961 ggcttactgg cacccggcaa gcaggccgat atcgtcctgt tgagcgatgc gcgtaaggtc
     1021 acggtgcagc aggtactggt gaaaggcgag ccgatcgatg cacaaacctt acaggcggaa
     1081 gagtcggcga gactggcaca atccgccccg ccatatggca ataccattga tcgccagcca
     1141 gtttccgcca gcgactttgc cctgcaattt acccccggaa aacgctatcg cgttattgag
     1201 gtaatccata acgaactgat cacgcactcc cgctccagcg tctacagcga aaacggtttt
     1261 gatcgcgatg atgtgtgctt tattgccgtt cttgagcgtt acggacaacg actggctccg
     1321 gcctgtggtt tgctcggcgg ctttggcctg aatgaaggtg cgctggctgc gacggtcagc
     1381 catgacagcc ataatattgt agtgatcggt cgtagcgcag aagagatggc gctggcggtc
     1441 aatcaggtga ttcaggatgg cggcgggctg tgcgtggtcc gtaacggcca ggtacaaagt
     1501 catctaccgt tgcccattgc cgggctaatg agcaccgaca cggcgcagtc actggcggag
     1561 caaattgaca ccttgaaagc cgccgcccgt gaatgcggtc cgttaccaga tgagccgttt
     1621 attcagatgg cgtttctttc cctgccagtg atccccgcgc tgaaactaac cagtcagggg
     1681 ctgtttgatg gtgagaagtt tgccttcact acgctggaag tcacggaata ataaaaaaaa
     1741 gcccggcgtc atgccgggca aaagtcacca gttacgttta tgccactgtc aactgctgaa
     1801 tttttttctc gcggcggatt ttgcgttctt ccatcaccgc cactatcgcc atcaggcaga
     1861 tacaaccaat cgcggcgata tccagcgcgg cgaaggtgcc tgcccagccg gtaaggccaa
     1921 ataccggcgt cccatcggca atcattccca gacctaactt ggcaaagctg tcaccaatca
     1981 ggtaagcgaa ggtgccttta ataccatcgg cagcgccaat cgctttttta ggtacaaagc
     2041 caacagccgc cacgccaatc aacaattgcg ggccaaagac caggaaaccc aacgcaaaga
     2101 gagaagccag gtagatatat tcgttgctgg cgtgttgata cacaccgagc gtggcgataa
     2161 tcagcgccag cgcgatacag gccaccaggc cacggcgacc gttcgccagg tcagagagcc
     2221 agccccacag cagcgtaccg accagcgcac cagcttcaaa cagcgtaaag ccctgaatcg
     2281 ccaccgcttt agagagtttc agttcctgga acgcatacac ggttgaccac tggtcgatac
     2341 caatacgcac cacatagagg aaaatgttgg caaagcacag cagccagatc actttgtttt
     2401 tcagcacata ctcaacaaag atctgccact tggtcatatc ggtagattct gtctctttat
     2461 cctcttcact gatctcctcg ccaaacagct cttcagcttt gccgaggcca taagattccg
     2521 gggagtcgct gccgtaacgc agaccgataa aaccaacaat cagcgcgata atcgacggga
     2581 aaataaacat gccgatgaca tgaccatcga acaggtaatt tgcgccgaac agcgccacac
     2641 ctgctgcgcc tgcaccacca aggttgtgag aaatgttcca gaaaccgagg aatgtcccgc
     2701 gtttacgacg cggcgtccat ttggtgatgg tggagtaact gcacgaaccg ccggtactct
     2761 ggaaaaagcc gcttaaggcg tagaaggcaa tcatcaggaa caggctaacc gagccgctgc
     2821 ccatactggc actgaagccc agcatacaaa tggcagacag gatcagcatg aacggcagga
     2881 attgtttggt gtttttgccg tcggcgtagt aagaaaccag cgttttaccc acgccataag
     2941 tgatggagaa gcccaggccg atcatcccca gctgcgtcat gctcaacccg taggtcgaga
     3001 tcatgtcgtt ctgcgcgatg ttaaagttct tgcgaatcag gtacatcgta aggtagccga
     3061 taaagaccac caggtaggat tgcatgaacg gtttgaacca cattttgcgc cgcacttcga
     3121 gcggaaggtc cagggtcggc ttgcgaacct ggtttaggaa agccagcatg ggttactcct
     3181 gatttccctt catacttcaa gccgcaggtg ccttagctgc acgcattcct cccggtcaca
     3241 tagttcacta tgctcccggg actcacgcgt ttgctgcgtt cctgcaactt gaattatttt
     3301 gggaaagaat taatgaatga tggcattgta aaaatcagca gcaaatcagg cctgagatag
     3361 cgtccaggca aaacctggaa aatttcttag ttttgccgga tatgaagtga aaaggtgaga
     3421 tgcatcacgc ttctcgcggt gtctgggcgt tcaaaaaggg cagtaacagc agtgcggaaa
     3481 ccccggcggc gatagagata accacaaaaa atccgctcca gtgccaggta tcgagtactt
     3541 tcgccagtgg ccaaccagca agcgacgccc ccagataagc aaacaagccg acaaaccccg
     3601 tcgccgcccc tgccgcctct ttgtgggaac actctgccgc cgccatgccg attaacatct
     3661 gtgggccaaa gacaaaaaaa ccaatggtga agaagcaggt tgcctgcatc acgtagctgg
     3721 caaatggcat cagccacagc gagccgactg aaagcaaaat tccggcggcg aaaatcaaat
     3781 tcatcggccc acggttgccg ttaaacaatt tgtccgagcc ccaaccggct accagcgcac
     3841 cgataaatcc acccagttca aacatcgtca ctgccgtatt cgccgtgacc agatcgacgc
     3901 ccagcgtctc ggacatatac aaattgcccc agtcgttgat tgccgcccgg accacataga
     3961 ccagcacata gcaaaacgaa agcagccaga tatacggatt cagcaacaca tatttggtga
     4021 ggatctcttt acgcgtcagc cctgcccctt cttgttgttg agctatctcc agcgcgtcgt
     4081 gccgccattc accgaccgct ggtaaaccta acgcctgcgg gcgatcgcgt agccgccagc
     4141 agagaaaaat ccccacgacg atcgccatac aacccgcaat catcatcccg gcacgccagc
     4201 cgtaatgcag cgcgctcgct gccatcacaa tgggaatgag tgcgccgccg acgttatgcg
     4261 ccgtgttcca taatgcccac caaccgccgc gctcggtacg agaataccag gcggttaaca
     4321 gacgcgcaca caccggtgaa ccccagccct ggaaaaaggc gttcagcacc cagagtacgg
     4381 caaacgccca tagcgacgtc gagaagccaa acagaatgtt gatgatgccc gtggcaataa
     4441 gccctatccc cataaaataa cgggcatttg agcgatcgct gacaatgccg gagacaaact
     4501 tcgacacgcc gtaggtaatg taaaacaggg tcgctaacag gccaatatcg ctacggctga
     4561 gcacgccgtt agcaaggatt tctggtacgg cggcgttaaa acttttacgc gtgaagtaaa
     4621 acagcgcgta acccagccag atggtcagca gaatatgccg acgccagtag cgatagcggg
     4681 catcaatttc atgtttatca gtcattaatg gcgcatcggc aggcgctttc agaaacggca
     4741 acatcatcga accttagaca taacgttgag gtagagaaac gctgacacgc gtgccgtgca
     4801 gacaggaaat gtgtaatgtg ccgcccagcg ccgttacgcg ctcgcgcatt ccggtgaggc
     4861 caaaaccttg ttgcccggaa cccggcggca aaccgctgcc atcgtcttca atcaccagca
     4921 tcaaccgttc atcctgctgc cagccttgca gcgtaaccgc gctggcatcg gcatgtttca
     4981 caatgttgtt cagcccttcc tggcagacgc gaaacagcgt cacgcgctgg ttttcactta
     5041 acgctgactc atcgatacgc cattcgagat ggctgacaat gccgcgccct tccagctcca
     5101 tttcccgcat cagtgagcgg atggcctgct ccagggtgag atcatccagc tggcgcggac
     5161 gtaaccgccc taacaaacgg cgcaccgcat cgtaaacacc cagcgacagt tgttcgatga
     5221 gctgcccgct ctgcttcacg ctggcattat ctgccgccag tcgctggaca atgcccgctt
     5281 gagtacgaat agcagtgatg gtctggccga tatcatcatg cagctcacgc gccacatcgc
     5341 ggcgcacgct ctcttcagtt tctaacaaac gttcagccag atgctgattg cgcgccagtt
     5401 ctttttgcag cgactggtta agttcacgca accgctggat gccagcgcca agcaacaacc
     5461 ctgtcagact ttgcaccagc agcgagagca ataaatccac cgaatgatcg cgccaggttt
     5521 gactggcgat cagcgcgatg gcgttcatca acgtcgcaat tagcgcccct tgccagccat
     5581 agtgccaggc cagcgcgata atcggcagcg ccaggcagaa tggcgtaaag cgcgacagtt
     5641 cgtccggcaa tcccaactgg agccagagac tgataacaaa cagcagcaag taccagacca
     5701 gatgccgccc acgccagttg atcggttgag aaactaacga cgggccgagc ggcagccagg
     5761 tgttattggc gagatagtgc cagaacacca gacatatcgg ggccagcgtc aggccgccag
     5821 ttaaagtcag caacagcgca ttccacgact ctttgccgtg ccaaagccag ggcagcgact
     5881 gcaacaacgc cgccgccgtt aatgccgccc cttgtagcag caaggtgcgc cagtcacgtt
     5941 gatggcgata gcgcgagatc agcgccacgg gcagtaacgt cagtaaacta ccgatcatca
     6001 ataagaagaa atgggttaaa ccgaccgcct gcgttaacca gtaaatcagc agccactccg
     6061 cgcccagcaa caccggccag tatccgcgcg ggcattgcag cattagcccc agacgcagac
     6121 caaacggaaa taacagcacc gccatatcag ggcgctcaac caggtgcagg ctgatactcc
     6181 acaggcaaaa ccatgcggca gagaagataa aaaagcaggc aataacggta attaagcggg
     6241 agaacaacgt cttcatcacc agccatcaaa catacggcgc gccagttcaa cgtcgttact
     6301 gacgcccagt ttttccatca gattggcgcg atggacgtgt accgttttcg gtgacaagcc
     6361 cagttcggcg gcaatctctt tcaccgccat tccttgcgcc agtttttctg ccacctggcg
     6421 ttcgcgtttg gttagcgggt cctggcgacc ggatgccagt ttaatggcaa tatccggcgt
     6481 cagataacag ccgccagtgg caaccgtatg caccgcagca atgagttcat ccgggctaca
     6541 gcgtttggag agaaagccgc gcgccccggc gttaagcgcc tgctcaacca gcgccggact
     6601 gtcgtgaacg gagagcataa ttgtcgccat gcctttcggc agctggctta gaagctccag
     6661 accggagata tcaggcatgg agatatcgca aatacacacc tgcacaccgc gccccggcag
     6721 ccccgccagc gcctcgcgcc ccgaaccaaa ctcggcaact acctgcaaat caggttccag
     6781 ccccagcagc tgcgcaaagc cggagcggac gataaggtga tcgtctataa gggcaacggt
     6841 gatcatgatc ttgtcctgga gggtaataaa acgcgcttac cttaacgata agcgcgatgt
     6901 tgttcaagcc ttgagcggtt actgaaaaaa caccgcaatc ttgttaaaca tggtcggatc
     6961 ggactgatta cgctgcactt tcaccacatc ttccagcttg tcgatttggc ttatcatctg
     7021 ctccagacgc tggtcgtcat tgaccagtag ccagatgtgg cttttgtcgc tgtcctgaat
     7081 cggcaggcag agaatacctt caacgttaaa agcgcgacgg gcaaaaaggc cacaaacgtg
     7141 ggtcattacg cccggatggt tgcgaacggt gagttcgaga atcacgttgt catgagttgt
     7201 gttttgcatg gcttattccc ccaccatttc agtattcgcc gcacctggcg gcaccatcgg
     7261 gtacactttt tcttcggcat caatgcgcac gtggatcagc gccgggccag ggcgattgat
     7321 gatttcctgc aatgcagcct gcggatcggc ttcgttattc aaatcacagg tttcgaggcc
     7381 gaatcctgcg gcaatctgca taaagttgat tttgcccgga taggtagcgg caaaaacgcc
     7441 ctgcttgtag aacagactct gttgctgatg caccagcccc agcgcttcgt tgttcatcaa
     7501 aatgatttta acatccagct gattttcact ggcggtcgcc atctcctgaa tattcatcat
     7561 caggctgccg tcgccggaga agcacaacac tttgcggtcc gggttcgcca gcgccgcgcc
     7621 aatcgccgca ggcaggccaa aacccatcgt gcccagcccg ccggaggtca gccactggcg
     7681 tgggcgattg agcggatagg cttgcgccgt ccacatctga tgctgaccca cgtcagtggt
     7741 aataatcgcg ttgtcatcca cgcaggcggc aacggcatta atcaggccgt aatggcttaa
     7801 tggatcgcac gctttcggga ttggacacgg gaattcacgc tgcaaatccg ccaccaactg
     7861 gtgccactct gcacgcggtt gcgcttccac cagcgggatc aactgcgcca gcacgtcatc
     7921 aacatccgcc tgaattgcca catgcggttg cttgattttg cccagctctg cgcggtcgat
     7981 atcgacatga ataattttgg cattcggaca gaactgctcg gttttgccaa tcgcccggtc
     8041 atcaaaacgc gcaccgagta caatcagcag atccgcctcc tgcaaaatat agttggtgct
     8101 gcgcacaccg tgcatcccca gcatacccag cgacagcgga tgcgcttttg gcaacatacc
     8161 cagtgccatt aaagtcatgg tggtaggcag ttgcgctttc tccgccagtt cacgcactcg
     8221 cgcaggcgcg ttgatcacac caccacccag ataaagcacc gggcgtttgg cggcgttaat
     8281 catcgccgcc gcgtcacgaa tactttcttc gctaaaggcg ggtgcggcgg ctttttctgc
     8341 catagcgggc tgtgtttcaa tctcaaaaac tgccgtttgc acatccttag gaatgtctat
     8401 ccacaccggg ccagggcggc ctgattgcgc aatacggaag gcatcgctca tgacctgcgg
     8461 gagttcttcg atatgtctga ccagatagtt gtgtttggtg atggggatag agatgccgta
     8521 ggtgtcgact tcctggaagg cgtcggtgcc gatcatcgag gcgggaacct gaccagtaat
     8581 gcaaatcagc gggatggagt ccagccgtgc atcggcaatg gcggtcacca ggttagtcgc
     8641 ccccggtccg ctacaggcca tacagaccgc cggtttaccg tcggtgcgcg ccattccctg
     8701 agcgataaac cccgcgccct gttcatggcg agccagaata tggcggattt gcgtgctttg
     8761 gcttaaggca tcgtaaacag gcaggataga accgcccgga atgcccgtca caatcttaat
     8821 gccctgctgt tccaggaaat gaacgataaa ttctgcgccg gtaaagcgct tacgcgtcga
     8881 tgttgtgccc gaacttgcca tgctccagtc cttttcttct gggccgactt tccgggaggt
     8941 gcttaaacga aaaaccccgc ccggtttgcg ccggcggggt tttggaatcg tgtgttgttc
     9001 cagtccctac ggcgcattgc cgacgaccac caccacacgc acgacgacca ctgcggcgga
     9061 tggcgcagtt ggtagtagtt ttgcgttgaa catggaagta gtcattgggg accttgttgg
     9121 ttttgtgttt aacaatattt atacaagcac agctttacag gggagacaat ggaaaatttt
     9181 tcagcaaggg aaaattgagg ggttgatcac gttttgtact gaattgcagg taacaaaaaa
     9241 ccccgccgga gcgaggtttc gtcagtcgcc tgcggctggt aaccgcaaag cacactgtat
     9301 tatgtcaaca ctgaaagtat acgtgttccg cgcagaacgc gcaatttcgg cacgaatttt
     9361 gacgtattta gtgcatagtt gagtatcgat cacagtttgc gttttgtcca atattactgt
     9421 ttatttatac agtaaacttc tataatatca ctgtacgcaa tgtgttatgc gggggccgca
     9481 tcgttacccg gcgcactaag tcctggctga aacgggtggt gccgtcagcg ccttaacccc
     9541 gcgtgagcac actgtgttat gtcaacaagc acaacgtttc tccttgagat accgcgtgca
     9601 caacagctgg caacaggcag cggaaaggta cgtcagctgg cagtgctcct gaaccacagg
     9661 agacgcgtat gagcctggtg gatatcgcca ttcttatcct caaactcatt gttgcagcac
     9721 tgcaactgct tgatgctgtt ctgaaatacc tgaagtaatt cagattcaag tcgcactaaa
     9781 ggggagcggg taaccgctcc ccttttatat ttagcgtgcg ggttggtgtc ggatgcgatg
     9841 ctgacgcatc ttatccgccc taccatctct cccggcaaca tttattgccg cttttgttta
     9901 catattctgc cgctaaacaa ttccccattc ctggcgtata tctggctaac atttatcaat
     9961 gtgatagatt cctctcccgc atttatggga atgcgtagtg acttattcta attattttta
    10021 taaaagcatc cgtgataatg aaaaggcata gaaacgtcaa tttgttattg atgttggtat
    10081 tactcgtggc cgtcggtcag atggcgcaaa ccatttatat tccagccatt gccgatatgg
    10141 cgcgcgatct caacgtccgt gaaggggcgg tgcagagcgt aatgggcgct tatctgctga
    10201 cttacggtgt ctcacagctg ttttatggcc caatttccga ccgtgtgggt cgccgaccgg
    10261 tgatcctcgt cggaatgtcc atttttatgc tggcaacgct agtcgcggtc acgacctcca
    10321 gtttgacggt attgattgcc gccagcgcga tgcagggcat gggcaccggc gttggcggcg
    10381 taatggcgcg tactttgccg cgtgatttat atgaacggac acagttgcgc catgctaaca
    10441 gcctgttaaa catggggatt cttgtcagtc cgttgctcgc accgctaatc ggcggtctgc
    10501 tggatacgat gtggaactgg cgcgcctgtt atctcttttt gttggttctt tgtgccggtg
    10561 tgaccttcag tatggcccgc tggatgccgg aaacgcgtcc ggtcgacgca ccgcgcacgc
    10621 gcctgcttac cagttataaa acgcttttcg gtaacggcgg ttttaactgt tatttgctga
    10681 tgctgattgg cggtctggcc gggattgccg cctttgaagc ctgctccggc gtgctgatgg
    10741 gcgcggtgtt agggttgagc agtatgacgg tcagtatttt gtttattctg ccgattccgg
    10801 cagcgttttt tggcgcatgg tttgccggac gtcccaataa acgcttctcc acgttaatgt
    10861 ggcagtcggt tatctgctgc ctgctggctg gcttgctgat gtggatcccc gactggtttg
    10921 gcgtgatgaa tgtctggacg ctgctcattc ccgccgcgct gttctttttc ggtgccggga
    10981 tgctgtttcc actggctacc agcggcgcga tggagccgtt ccccttcctg gcgggcaccg
    11041 ctggcgcgct ggtcggcggt ctgcaaaaca ttggttccgg cgtgctggcg tcgctctctg
    11101 cgatgttgcc gcaaaccggt cagggcagcc tgggattgtt gatgacctta atgggattgt
    11161 tgatcgtgct gtgctggcta ccgctggcga cgcggatgtc gcatcagggg cagcctgttt
    11221 aagctcacgt caccgcagca tcgtcatcag ctccatggga gaacgatgct gctttatcag
    11281 atcacgcatc acccgcatat gcggcgcgga gtaagaataa aacgcctgat agcccgcaca
    11341 aagcacgttg tctggtttat cttcccgagc gtgcgggcac agccgatgcc agtcgcacgc
    11401 ctggcagtca cggcgcggtt gcgacttttc gctgccacac catttttgta aggtggtgtc
    11461 aaaaagttca atcgaaatac gactgatatc tgcccgcacc caacactcga acagggcaat
    11521 taaaaatttc ccccacagct ctgctgtcac cgaagcggcc gtcagatcgc cccggtcatc
    11581 aaattcaaga tggggaataa gctgtacgga gcggatgccc agctgtttga aattgtgata
    11641 aaaatcggca ggttgcagag catccttata ctctacaacc agtttatgcc tgtcctcttc
    11701 agcatcaatg acctgacttc ctgtcatgcg actctctcaa taaatacgat aagttgatgt
    11761 atatgcgtta cagcaaacag tattgcgaga gataacaccg cgagggagat caaaaatttg
    11821 tcacggacta cgtgaaattg ggaaaaatcg ctattcgtga catccattaa attgcgatta
    11881 cgggtgtagt gccagaggat cagcgccacg atggcgagga tgccaatcga aatccagaat
    11941 aacatgcccg cctggtgcca gttgtgtttg atagccaacg ccatcaacgc gccgtagccc
    12001 agcatggtac gaaaccacgc cagtgatgtg cgttccggct gcagccctgg gtcggcaatg
    12061 cgtctggctt tgcggctatc cggcatacaa caccagtccc atgacgatca ctgccacgac
    12121 catcaatatt aaactgatga ttaataagct gttggtatag ggcaggtcct ctttcaggcg
    12181 catcgctttt tcattgcgta accagcgcaa gtagccatac atcgctaacc cgccggaaaa
    12241 cagacacaac aacaacgcca gcagttcgcg gatgacgggc gtggcgaaat ctggcgcaag
    12301 ttgatcaagc ccaacccccg ccgccagaaa tcctagtgca gtacggatcc acgccagaaa
    12361 agtacgctca ttagccagcg agaagcggta atcgggcgct tctccgaggc gggaaatttt
    12421 cattgttgct ccttgtaaac tgttcctcgc cgaaaaatta ggccaggaac agcaataatg
    12481 catcagaact tctgtttttt ctcttccacc ttcacccctt gtgtcggtaa accggtgtca
    12541 tagtcgcgta ccactggaga ataaccatcc tgtgggcgtg gacgaaacgc ccccatccag
    12601 cgcggttgtg catctttgcg ccacggacgc aggttccatt gataactgcg gaacggatcg
    12661 cgaattttgt ccatataatc caataaggcg tcatgcattt tgctgcgaac gtcggcaaaa
    12721 tggatatcat cgatcaggtt atgcatttca tttgggtcat tacggcgatc gtaaagttca
    12781 tcactggtga agaggttgag caccagttta aagtcatccg ttacccagca acgcaccgga
    12841 ataaaaccgc caaagctgtc atgctcaatc tcgtagcgat taaattccac caacacgcca
    12901 cgtggttctt ttacggcaag gatattatcc cccggcagaa tctctggttt ttcaatatct
    12961 gccagcgcca tcattgtcgg cagtaagtca atatgactga ctggcgtatc gacctgtcgc
    13021 cgctcccctt gcggcgaacg gatgatcagc ggaatgcggg tgatgtcgtc atacatcgcc
    13081 gcccctttac tgatcagctt atgtgcgccc atcatttcac catgatcgga ggtataaatg
    13141 acccacgtat tttcacgttg ctctggcgtt aaggcattga tgacacgtcc gatttggtca
    13201 tcaacaaagt cattgcaggc aaaatagagc ggatggtgat aaagcccgtc atcaccgact
    13261 ggcgatggca tcgcctgcgc ccataagcgg tgatgttccg gtttgttcgc caggtcatcc
    13321 tgtgatttct cgccaagttc gtagtaaaaa tcagcgtatt tctctaaata ctccaccgga
    13381 caggtgaacg ggtgatgcgg ctcatcatac gaaaccacca tcaggaaagg ttcgtcggcg
    13441 cgcgcgggct gttgcagaaa atctaccgcc cgattgctga tgcggtgcgc ccaggtgaag
    13501 gtttcgtcga tatggttcgc ctgtaaatcc tcaacgctgt ttaggccatt gcgccacagg
    13561 ctgatctctt tttccgtcag ttcgctaagg tagttcgccc catcgaacca gtaatcagcg
    13621 tcccactccg gcggacactc gccagtgccg aaatagtcat gaccgtcgag atgccatttg
    13681 ccaatgtaac aggtgtgata gcccgcatcc ttaaagtagc gccccatggt ggaaatgttt
    13741 ttacctggcg cgacgttgtt ggtccacggg ccggactggt tagcgtagat accggtaaac
    13801 agcccggcgc gcgctggtgt acaaaccggt gaacaggtat aggcggaatt aaagcgaata
    13861 ctttccgccg ccagactatc aatattttgc gtattcagcg gcttaccgct atagcaaccg
    13921 accatattgg tggcctgggt atcggtcatg atgaacagaa aattggggcg tttcataggc
    13981 tatccttcgc gtcgggagta aaggttacgg ctgggtcgtg acgatgccgc cagctatcaa
    14041 aaatcaggta gacaatcact acggcggcaa tgaaataact gatcatcgct aaccagcgcg
    14101 taccgtagcc gccgaattca gccagcccgg catagacgcc aatcatcgcg aacaggatgc
    14161 ccattgacgc gatcttgacg tttttccacg gtttcatgtc caccgcaaac gcatctttga
    14221 aggtgaacgg cgtggcgcgc ggtttgataa aaccgatcac cagcatcacg accacgttga
    14281 tgcagaacgt acaggccagc acatagagga aatggaagtc gaacttcacc agatagttga
    14341 tggtgatgta gctgattatg ccaatcccca tcgccacttt tgccgccagc gccgggatgc
    14401 gcgggaagaa aaagcccata atgatgatgg taaccagcgg cacgttgtaa atgccgttga
    14461 gctgtttcat ccagctatac agcccctgcg gcgcgttggc gatccacggc gctaccagca
    14521 ccgaaacgat ggcgataaag aaaccaaatt tgcgcccgac ggtgaccagc tgctgcggct
    14581 cggcattctg gttaatgatg cgccggtaaa tacccatact gaataacgta ctggcgctat
    14641 tcagaaagcc gttgaaggta ctgatcaccg caccaaataa caccgcgccg aagaaaccca
    14701 ccagtggcac tggcagaacg ttattgacca gcgtcgggta ggccatgtcg gctttcggta
    14761 gatcctgata cagatgaaac gcaatcaacc ctggcagcac cagtaccagc gggtcgagca
    14821 ttttcagcac cgccgttaac agcgccccct tttgcccttc cgccaggctt tttgacgcca
    14881 gcgtgcgttg cacgatgccc tgatttgtac accagtaaaa ggtgttcacc agaatcaaac
    14941 cggtaaatgc cgcgccaatc ggcaagggat cggtcgggcc gccgactgag tttaatttct
    15001 cggcgtgaac ggtggtgagt tgctcaatgc cctgcataaa gctgcccttg cccatcgcga
    15061 tcaggccaaa taccggcacc atcaacccgc caataaccag cccaataccg ttaatggagt
    15121 ccgccactgc cattgcgcgc agtccgccga tcaccgcata caaaattccc gccagaccaa
    15181 gcaaaataac cagtaaccag atagccgcac catcggaaag ttgtagcgac tcacccacat
    15241 gaaacaggct gttgagcgcc aacgcgccgg aatagagaac aatcggcaga aagcagacgc
    15301 cggtggcaat gaggaagcag aagtcgatga taatacgcgt cgttttatca taacgttcct
    15361 ccagaaaatc ggggatggtg gcaatgccgc gcttcagata gcgcggtaaa aagattagcg
    15421 cgaggaagat cagcgtcact gcagaggtga cttcccagcc catcaccgac atgccacttt
    15481 tgtaggcctg cccagaaagg ccgaccagtt gttccgtgga aaggttggtt agcattaacg
    15541 aagcggcaat aaccggcgct tttagtgaac gtccggcaag aaaatagcct tgttgcgatc
    15601 cggtatctgt tttacggacc ttccaccagg tgatgaccgc taccagcagc gtaaaaccga
    15661 caaaactcaa gatttgtaac gaattcatct catagccctt atttatgttt atgtcccgga
    15721 cgcatgaata tagggagagg taaaatgcgt cgggtcaggc agtcagattc ttaaaataca
    15781 aacgtcgtat ccctgaacgg attgttttct gttaagttca ggttgtgagc ataattcgcc
    15841 ggagggatat taaaatgaat ggaaaattgc aaagttcgga tgtaaaaaac gaaactccgt
    15901 acaatattcc cttattgatt aacgaaaatg tgatctccag cggaatttct ctgatatcgc
    15961 tctggcatac ctacgccgac gagcattatc gggtgatctg gccgcgggac aagaagaaac
    16021 cgcttatcgc caactcatgg gttgcggtgt ataccgtaca aggatgtggg aaaattcttt
    16081 taaagaatga cgagcaaata actctgcatg gcaactgtat tatattttta aagccaatgg
    16141 atattcactc ttatcactgt gaaggtttgg tctgggaaca gtactggatg gaatttaccc
    16201 ccaccagtat gatggatatt cccgtcggtc agcaaagcgt tatttataat ggcgaagttt
    16261 ataatcagga actcaccgaa gttgctgaat taattacttc accagaagca ataaaaaata
    16321 atctggcagt cgcttttctg acgaaaatta tttatcagtg gatttgtctt atgtacgcag
    16381 acggtaaaaa agatccacaa cggcggcaaa ttgaaaaact aattgccact ttacatgcca
    16441 gtctgcaaca acgctggagc gtagctgata tggctgccac gatcccctgt agcgaagcct
    16501 ggttgcgtcg tctgttttta cgctataccg gcaagacgcc gaaagaatat tacctcaatg
    16561 cacgtctgga tctggcgcta tcgctattaa agcaacaagg gaactcggtt ggcgaagtcg
    16621 ctgatacgct caacttcttc gactcctttc atttcagcaa agcctttaag cataaatttg
    16681 gttatgcgcc gtcagctgtg ctgaagaata cggacaggta ataaccagaa tggcgagtgt
    16741 gtaaatgact cgatgatgtt cagaaatacc aggtggagta ccatttacgg cgactccacc
    16801 cggaaaagag ttaaccgata ctccagaaga gcaccgccag taattggggg gtgataatgc
    16861 gcaggaacat caccaacgga tagacagtgg cgtaagaaag cgccgccgca ccgctggttg
    16921 gatgaagatt attagcaaac gccagcgccg gaggatcggt catggagcca gccagcatcc
    16981 cgcacatggt caggtaattc attttggcta acatccgcgc cagaatgcca acagtaatca
    17041 gcggaacggc ggtgatcagg gcaccataac caatccagct tagcccttcg ccatccacca
    17101 gtgtatggat aaaatcgccg ccggatttca ggccaaccac cgagagaaag agcacaatgc
    17161 ctaactcgcg tagcgccagg ttagcgcttg gtggcataaa ccagtacagc ttgccgatac
    17221 tgccgatacg cccgaggatc aacgccataa tcagcggacc gcccgccagc ccgagtttca
    17281 acgccgccgg gaacccaggc acaaagacgg gaatagaacc taacaatacg ccaagcccga
    17341 taccaataaa cactggcagc atctgaacct gttgcagttt ttgctgcgca ttccccagca
    17401 cattggcaac ggcatcaatt gcggacggac gccccaccag gttgaggata tcgccgaact
    17461 gcaggctgat atcgctactt gcaactaatt caacaccagc acggttcagg cgcgagataa
    17521 caacgtcata gcgttcttta aagtgcaggt cgcgaatacg tttaccgagc acgttttcat
    17581 tggtgaccac cacacgctcg acgcgcaagt cagtgccttt cgtagacagc gaggtatcga
    17641 cctcctgacc aatcaccagt tgcgcattat gtaaatccgc aggctgcccc accagatgca
    17701 gcaaatcgcc cagttgaata acggtttctg gcgaaggcac ttttagggtt tcttctcgtt
    17761 tcaatcgtga gcaaataact ttatcgccat tgagaatcgg cacatcttta atggctaaat
    17821 tatgcaaatt aggattctca actcggatgt taatcgtcct gattagcgca ccgccgttgg
    17881 ttcgcgtgga ctcgtgctgc tgggcctcag tctcaacatt gacgcggaaa ataacccgca
    17941 gcatccacat agtgaacaaa atgccgcaaa tcccaaatgg atacgccatc gcatagctca
    18001 tccccatttg atcgaccatc gccatcggtg tccccaggtc gcgcaagatc tgctgccctg
    18061 cccccagtgc tggcgtattg gttaccgcac cggagaaaat ccccagtact accggcagtg
    18121 gaatatcaaa cagtttatgc aggatggcgg taaccagccc cccgatgatg acgatcagaa
    18181 cagcaaacag gttgaggcgt aatccggaga cgcgcaatga ggcaaagaag cccggcccta
    18241 cctgaatccc gatggtataa acgaacagga tcaggccaaa ttcctgaata acatgcagca
    18301 tatcgctact taatgtcatc cccgcctgag aaacaaaatg gccgacgatg atcccaccga
    18361 acagcacgcc gccaatgccc aatccaacac cgcgaaactt gacgttgccg ataaacaacc
    18421 cgacgaccgc caccaaagcc agaatactga ccgttaatgc tatatcactc atcgtccatt
    18481 tctcttgaga taacatttta attgctacgg attatggctg aatcggtgaa gcagatttgc
    18541 cttatagcgc acattatcac gggaaagaac gtgccgaaat atcttaaaca ggcgaaaatt
    18601 aacgcattgt tgaagaagaa agacaaaaaa agccccacca gaatggcggg gcaaagagaa
    18661 tagctagtta gctatttaac gcgggacgtt cgctgatagc gatacgctgc gctgcgatgg
    18721 gttcaggctc attacgaatt aaatcaatat gcagtaaacc gtttacgaag gttgcaccag
    18781 agacttccat attttcagcc agcgtaaagc tcaggctaaa tggctgattc ataagccctt
    18841 gatgcagcca ttttttctct tcttttggct gctccggcgt gccttttacg ctcaggcgcg
    18901 taccttccag ttgaatctct aaatcttcct gacgaaaacc tgccagcgca agggtaatgc
    18961 ggtagtggtt atcgtcgctt ttctcaatgt tgtacggcgg gaagctctgg ctttcaccgg
    19021 cgttttgcag tgcgttggcc agtttgtcaa aaccgatcca ttgacgcatc agtggggata
    19081 aatcgaagtt acgcatagtc atttctcctt ctaagaagcg agtaagtacc tgcaaatccg
    19141 aagattcgca tatgctccct gacggcgagc atggagatgt caggccgcgc aaggcggcct
    19201 tagggaatta gttgatttcg atacggcgcg gttttttcgc ttccggaatc acgcgttcga
    19261 gatcgatata cagcaaacca tttaccaggt tagcaccacg aacatgaatg ttctcagcta
    19321 actggaattt gcgttcaaag ttgcgttcag cgatgccctg gtacagatag gtgcgctctt
    19381 tttgttcgtc ggcgtgagca cctttcacca ccagcagatt atcctgggcg gtaatttcca
    19441 gttcgctctc agcaaaacca gccacagcga tagcaatgcg gtaatggttt tcgtctacca
    19501 gttcaacgtt atacggaggg tagccgccat tactctggct ctggttgttt tctaagtggt
    19561 taaacaaacg gtcaaatcca atagcagaac ggtaaagcgg ggataaatca aagttacgca
    19621 taatcaatag ctcctgaaat cagcgagaat ctaagacctt ccacaatgga caggtcaggt
    19681 agccagaaca cccatcaggc gcgtcctcat cggctacgat gtaaaaatgg gtctggaaat
    19741 gaacttttca agccttatca gcggactttt ttttgcagtt tatggtctat tgcatagact
    19801 gagggggcag cacgaaacgt taaagtgcga tagccgccac agtgcgacgt aatgatgatg
    19861 ttattttcag caagggatcg ctataactca tcaggccaac acccacgatc aacagatgaa
    19921 tttattatga taagaaatgt gttgttagcg ttcatgatat gcagcggaat gacattactc
    19981 ggaggatgct ccagcgtgat gtcccatacc ggggggaaag aaggaacgta tcctggcacc
    20041 cgcgccagcg caaccatgat tggcgacgat gaaaccaact ggggcacaaa atcgctggcg
    20101 atactcgaca tgccatttac cgcagtactg gacacaatat tgctaccgtg ggatgtgttc
    20161 cgcaaagaca gctcagtgcg ctcgcgcgtt gaaaaaagcg aagccgacgc gcaagaaacc
    20221 aataacgtca ttccccccgc caaaatgcct gcaaactgag agctagcggt cagtttcggt
    20281 gatccacaac tgaccgtcct ccatccacgc caaccattga ccatccggcg agaaaaccac
    20341 ggcgtccgca gaaggtggat tggcgtggtt ttcggttaaa tactcaacct cgccgctttg
    20401 cgcatgggca caagcaatcc gattatccag cacaaatccc agccattcgc ctgacggatg
    20461 ccagttaaat gcagactgaa tatccgtttt gttatgggtt aactggcgcg gctcgccgcc
    20521 ctgtggcgag ataagccaca gttgcacaat gccgttatca tcacgcatta aaaacgcgat
    20581 ttgcgtaccc tgcggattac agcgcaccca gtggcgcggg acgttgacta atcccggata
    20641 agcccgatgg tgcgtaaagg ttaaacgtcg ctgcacgaca ccacgcggtg gcgcaggcag
    20701 ggttgtttcc gttccactta acggcacatc acctgccgct ttccagccag cttcatcttt
    20761 cggcaactcg acaataaaca gctccggcac tttctcgccc tttgccgaaa gtgtgtcgcc
    20821 aataaacgcc agcgcgtgat ttcctaccca tccttcttca taagcacgat tgatttcatc
    20881 actgccaggc tgtggcgtgg gcgtggtttt acttaccagc acgcaccagt ggctaccgct
    20941 gtattcacgc ggatgctgct tttgtacgtt gaccgggcca aacggtgcag caacaccgac
    21001 gtttcgcaaa tccagcgccg gatcgagttc atgcattaca tggtcgttgt aggtaaagct
    21061 caccctttca ccgttcgggc taaagacatg tacatggctg ccgccacgta gcgctcctgg
    21121 cgtatatggc gcggtaatat ccattgcatc gagattgctc accttgccgc tttcaacaat
    21181 cactccgcgc cgatgatgaa agtcgtaata ccatgtttca tcaggatttt ccgggccgtg
    21241 gatgaagaca tacttctctg acttcggatg aacggtcacc acgccgacgt atgcgccctg
    21301 tgacgcgcga tagataacct cgacctcgcc ggtatggata ttcacacgct caatggtctc
    21361 gccggtaaac gacgccccag aaggccgcac gtcaaacacc agccactggc tgtcgggcgt
    21421 ccaggtattg gtattggtga gcaggtgatt acgaggagca aaggttatct gtttcatggc
    21481 gcggtcctga tacaaaacgg ttcccgccat aatacttcac aaggacttca ctttcagaca
    21541 atagtctttt gccatcgtga tgaatgtgag tgaaaagatg gaacatttcg acgtggcgat
    21601 tattggcctc ggcccggcag ggtcggcgtt ggcgcgaaag ttagcaggca aaatgcaggt
    21661 gatcgcactg gataaaaagc accagtatgg tacagaaggt ttcagcaaac cttgtggcgg
    21721 tctgctggca ccggacgcgc agcgatcttt tattcgcgat ggactgacgc ttcctgtcga
    21781 tgtcatcgcc aacccgcaga ttttcagcgt caaaaccgtc gacgtcgccg catcgctcac
    21841 gcgtaactac cagcgaagct atatcaatat taatcgccat gctttcgact tgtggatgaa
    21901 atcgctgatc cccgccagcg ttgaggttta ccacgacagc ctgtgtcgaa aaatctggcg
    21961 tgaggatgat aaatggcatg tcatttttcg cgcagacggc tgggagcagc atattaccgc
    22021 ccgctatctg gtcggtgccg atggtgccaa ctcgatggtg cggcggcatc tctacccgga
    22081 tcatcagatt cgtaaatatg tcgccatcca gcagtggttc gcagagaaac atccggtgcc
    22141 gttctactcc tgcatctttg ataatgcgat aactgactgt tactcatgga gtatcagcaa
    22201 agacggttat tttatctttg gcggtgccta tccgatgaaa gacggtcaga cgcgtttcac
    22261 gacgctaaaa gagaaaatga gtgcctttca gttccagttt ggtaaggcgg tgaaaagcga
    22321 aaaatgcacg gtgctgtttc cctcacgctg gcaggatttt gtctgcggca aggacaacgc
    22381 ctttctgatt ggcgaagcgg cgggatttat cagcgccagc tcgctggagg ggattagcta
    22441 tgcgctggat agcgcagaga ttctgcgttc ggtattactg aagcagccag agaagctcaa
    22501 taccgcttac aggcgcgcca ctcgcaaact gcgtttaaaa ctcttcggca agatagtaaa
    22561 aagccgatgc ctgaccgcac cggctttaag aaagtggatt atgcgcagtg gagtggcgca
    22621 tattccacag ttgaaagatt atccaacgcg cttcacatcg cccaccagca ggatgtaaga
    22681 gagcgcgcca atcaacgcga cggcggagat ataaaccagt gcaggcgcga aaccgtaacc
    22741 ctgcgcaagg taccccacca ccagcggaac ggtgatgccg cccagaccac cggcaaagtt
    22801 aaacacaccg ccggttaaac caatcaggcg catcggtgcc agagaagaga ccagcgacca
    22861 ggttatcgaa gcaaaaccgt tgccgaagaa tgccagcgcc atcaggcaca taatcatcat
    22921 cggatcgttg gtgtagttcg cacccataat gcaggtggag atcagcaagc cgcagataat
    22981 cggcgtttta cgcgcaaagc ccagtgaaaa gcccttacgt accagcagat ccgcgaccca
    23041 gccagagagc aggacgccaa taaacgccgc gaggaatggc accgtggtca taaaacctgc
    23101 tttcagcgcc gtgattcctt tttcctgggt taaatagttc gggaaccagg ttaagaaaaa
    23161 ccacagtgta gaagccaccg caaattgccc aagatagacg ccgatcagtt tacgatggaa
    23221 caccagtttc cagtctttgg ctgttaacgg ctgacgcgcc tcttttttca ccggcgcatc
    23281 accatccacc agaccgccgc catcacgaat gtaatccagt tcagctttgc tgatgccttt
    23341 agtcaggcgc ggcggctgat aaaccttaaa ccaaatcagc gaccagataa tgccgatacc
    23401 accggtgaca atgaacaccc agtgccagct caacatttcc tgaatccaga tcagcagcgg
    23461 agtcaaaaac gccagaccga caaactgacc agacgtataa aaaccaacgg cggaagcgcg
    23521 ttcatgttcc gggaaccagc tggtcaccat ccggttattg gtcgggaaag cgggcgcttc
    23581 gaaaataccg gttattgcgc gcagaccaat taatgacatc aatcccgtgg caaagccctg
    23641 gaataaagtc gccactgacc agccaaatat cgcaataaaa taagtcacgc gtgaacctac
    23701 gcgatctaaa aaccaaccgc cggggatctg acatagtgta taaagccagg cgaaggccga
    23761 aaatacatag cccatttccg ctttggtaat accgaactct tcctgaatat gggcggaagc
    23821 cacggccagg ttagcgcggt caacataaca aatgaccacc gtaataaaga tcatcaccag
    23881 cgtcagataa cgccgacgcc ccggctttgc tgcattaacg ggaatatcca tagcgagctt
    23941 tctccagatt ttgggcatag cgaagccgct caccatgccc tgtaatttac agagggttat
    24001 atttttgtat tgctgtttta gtgcccgatg aggggcttac gtggcaggaa ttaccactct
    24061 gctacgctgt tatcttcatg acgccagagc ggattacgcc agtccggggc atttttactg
    24121 aactcaataa ctttagcttc gtcgatttcc acacctaagc ccggtttcgt taatggttta
    24181 aagaaaccgc cgaccatgct gaagtcttct ttgtttttca caaagtcgag taactccgcg
    24241 cctttgttgt aatgaatgcc catactttgt tcctgaagta cggcgttata ggaaacaaag
    24301 tcgatatgca ggcaagccgc cagtgcaatc ggtccgagcg gacagtgcgg cgcaagggtc
    24361 acgtcatagg cttctgccat tccggcaatt ttgtagcatt cggtaatacc gcccgcgtgg
    24421 gagagatctg gttgcagaat cgaaatacca cccgcctcca gcacacgttt aaaatcgaaa
    24481 cgtgagaaca tgcgttcacc cgccgccagt ggaatatgcg tttgtgccgc cagtttcggg
    24541 tagtattcgg cttgttctgc cagcaccggc tcttcaataa acagcgggcg atacggctcc
    24601 agttctttaa tcagcacttt cgccatcggc gcgctgacgc gaccgtggaa atcaagacca
    24661 aactcaatct gattgccaaa agcttcacga atttgtgcca cggtgttgac tgccgcatct
    24721 accgcgcggg agttatcaat tagccccagt tcttcacaac cgttcagttt gaaggtatcg
    24781 aagccgattt cgcgcagggt tttaatgccg tcgataacat ccgccggacg atcgccgccg
    24841 acccaactgt aggctttaat tttgtcgcga accaggccgc ccatcagttg ccagaccggc
    24901 gcattcagca ctttgccttt gatatcccat aacgcctggt cgatcccggc gatggcgctc
    24961 atcaggattg gaccgccacg ataaaatccg gcgcgataca tcacttgcca taagtcattg
    25021 atgcgcgatg gatcctgacc aatcaaatag tcacccagct cgtgaactgc cgcttccacc
    25081 gtgcgggcgc ggccttcaat cactggctcg ccccagccga ccacgccttc atcggtttca
    25141 attttcagga acatccagcg gggaggtaaa cgatacgtgg taattttggt gattttcatt
    25201 gcactgcctc tcgatacgcc ttaacaaatg ctgctgcctg ctgcgcggtg cgttctacgg
    25261 actgcccggc gcgatagaga tcgctgccta agcccgcccc tgcacaacct gcgtctatcc
    25321 actgcgccag gttttctggc gtcacgccgc caacggcaaa gactgcgatg tccggtggta
    25381 ataccgcttt taacgctttg atgtattgcg gaccaaaagc cgatgacgga aatattttca
    25441 gcgcctgagc gcccgcgtcg agcgcggtaa aggcttcggt cgccgtcgcg cagccggggc
    25501 aaacggtcat gccgtagccc accgcacggc ggatcacttc actatggata ttgggcgtaa
    25561 cgatgagctg gcagctcatc ctggcgagtg catcgacctg ttcaggtttc agtaccgtac
    25621 ctgcgccaat caacgcctta tcgccatacg catcaacgat ggcgggaatg cttttctccc
    25681 attgagggga attcagcggg atttcaaccg cgtcgaaccc ggcgtcaatc accgcgccaa
    25741 catgcgccag cgcctcgtcg ggcgtaatgc cgcgcaaaat ggcgatcagc gggagtttag
    25801 tttgccactg catgagcgat gctccttata ccagcctgaa atgccgtgtc gcccgccacc
    25861 gccgtcacgt cgcaacccat cgcctgaaag gcttgctggt agcgcgcggt cagcgatgtt
    25921 ccggcgacaa gggtgatggc gtgttgatgg gtcacatagt cgcgcatact ggcaacctct
    25981 gcgccaatca acaaaccaga gagaaattcg ctgacctgtt cgcggggaag tgttcccagc
    26041 acatgcgagg cgcgaacttc aaaaagctgc ggcaatatgg cgggagcatt aaagccacgc
    26101 tcaagaccag ccgcgaaggc atcggcagag ttttcctgcg gcggcaaacc tgcgccaatc
    26161 aatgagtgat ttaacagtaa atgatgtaat tcaccggtca tcacggtgcg aaaatcgttg
    26221 atttgctggc tatcggcctg cacccatttg caatgggtac cgggcataac ataaagagag
    26281 gaaggagcga gagcacgcgc gccgatcaat tgtgtttctt cgccgcgcat cacattgtgg
    26341 ttatcgtcat gagagacgca taatccggga ataatccaga tattgtcgcc aaccgacgtt
    26401 aattgatcgc caatagacga aaaacgggca ggaacagata aatacggtgc aactttccag
    26461 ccgacattgc tgccgaccat tcctgccatc accactggcg tattctcttc acgccagtcg
    26521 gtcgtgactt ctgctaacac cgcagccgga gattttccgt tcaggcgcgt gacgcctgct
    26581 tctgattgcc tgctgtccag gcagtggtcg ccctgataaa gccaggcgcg cagattggtc
    26641 gatccccagt caattgcgat gtagcgagct gtcatgtgat ttcctttaac cttcgtgtcg
    26701 agctggcgat catggtaagc gccgcctgct ctgccgcatc gccgtcctga tgccgtattg
    26761 catcgaacag cgccttatgt tcctggagcg tttgcggcat gttggcctca tcgcccatcc
    26821 aggttcgttc aaaaaccgcc cgctgcagcg aactgatcgc aatgctaagt tgctgtaaca
    26881 ccgggttatg caccgactgc agcaccgcct cgtggtagcg aatatccgct tcgttaaacg
    26941 cttcgcggtc ctgattgttg gcaatcatct cgttcagcgc cgattcaatc tgcgccagat
    27001 cgctggaggt cgcgcgctct gctgcccaac gggcaatagc cggctctacc aggtttcgca
    27061 cttcgctcat tgcactgata agccgtgggt catagtcgtt ttccagcacc cattgcagta
    27121 cgtcagtgtc gaggtaattc cactggttac gcggagccac aaacgccccg cgataacgtt
    27181 tcatttcaat cagccgcttc gccatcagcg aacgaaacac ctcacggatg atgttgcgcg
    27241 aggttgcaaa ctcttcacag agctccgcct cagccggaag tggagagcct ggtacgtatt
    27301 tgccgtgaac gatctgttta cccagcgtaa taacgatgcg atcggtttta ttgagagtca
    27361 tggagagtcc ttgtgctctg tatgttcttc tctactttac cccgatcgat gcataacgcg
    27421 gcaactttgt agtaccagcg tgatgacgtt cgcgtttgct gtgcgcctaa tgtagtacaa
    27481 acttatattg ttgtactaca atttagatca caaaaagaac aatacataaa aaatgacatg
    27541 cgtcgggcag aaatctgaaa agggatgtca ggcgctaaac aggagggaaa gaagagtatg
    27601 ctttcaacgg cttagctact cgtttaaagg attaatcatg aagttgaatt ttaagggatt
    27661 ttttaaggct gccggtttgt gttctttggc atttgcgttg accggatgta tcacctgggg
    27721 attggtttcc aatactgcaa gccgtccaca tgccgagcaa tggcggtcag acacgattaa
    27781 gggtttatcg ctggctgaag acagtaacgg tacgaaaggc tatgtttttg ttggtgaatc
    27841 gttggactat ctgttgacca ccggcggcga tgaggtggtt aaaatgctga acgatccggc
    27901 aatccatggt gaaagaatca ccgtttctga taacgcgaaa tttattttaa gcagcagtaa
    27961 taagaatttc tctggtgcta ttacgctcta ttacgactgg aacaacgaag aagataaggc
    28021 actggcaaca caatatggtt ttatttgtga tacccggcgt tgtacatgga tgctggacgg
    28081 tttgaaaggc tcaatccacc agaaaaataa aaaagcggat tactcaaacg tgatggtttt
    28141 ccatcaaccc tttacggtgg gattttatga gtacaaagca acagatggcg tcccgcgcgg
    28201 cctcgttaat gcgctgctgc cagtcactct tacgctggat atagtaacgt caccactcca
    28261 gttcctgatt ttatgcacga ccagaaattg ttaatcgctg cgggcaaacg cattcatgcc
    28321 gtttgcccat tcactaatta attcagcaca tacttctcaa tagcaaatgc cacgccatct
    28381 tcaaggttgg atttggtgac aaagttcgcc acttctttca ccgaaggaat agcgttatcc
    28441 atcgccacac cgacgcctgc atattcaatc attgcgatgt cgttttcctg atcgccaatc
    28501 gccatgattt cttccggttt aatacctaac acgtcggcca gtgatttcac acccgtacct
    28561 ttgttaacgc gtttatcgag gatttcgagg aagtacggcg cacttttcag cacggtgtat
    28621 ttttctttca cttcctgcgg aatacgcgcg atggcctggt cgaggatggc aggttcatca
    28681 atcatcatca ctttcaggaa ctgggtattg gggtccattt tctccgcttc gcagaacacc
    28741 agcggaatgg tggcaacgaa agattcatgc accgtgtagt agctgatatc acgattggcg
    28801 gtgtacagcg tggtgcggtc cagggcgtgg aaatgagaac cgacttcgcg agagagtttt
    28861 tccaggaaac ggtagtcgtc atagctgaga gcggtttgcg ccacggtgct accatcagcg
    28921 gccttctgta ccagcgcgcc gttataagta atgcagtagt cgcccggctg ttccatatgc
    28981 agctctttca ggtaattgtg cacacctgca taagggcgac ccgtcgttag cacgacattc
    29041 acgccacggg cgcgagctgc ggcaatcgca tttttaacgg cgggtgaaat ggtgtgatcg
    29101 ggcagcagaa gggtgccatc catatcgata gcaatgagtt tgatagccat gagttcccca
    29161 ggtagattgg ttcctgaccc atgctaacgc gattccgctc aaaaatcagt acaacacccg
    29221 agggaaaagg gggatgcaac gcgcgttgcg tgctcccctt ttgcttagcg gaagagtttc
    29281 cctttcagca gttccatgcc tgcggaaagc agatcgttat tggcttgtgg tgacacttca
    29341 ccttgcggtg agagtgcatc aataatcttc ggcaattgtt ctgccagtaa actggaagct
    29401 gtactggtat ccacgcccag tttttgcccg agatcggaca ccgcatttgt gccgagcgcc
    29461 gattccagtt gctcgccgct aactggttgg ttgccctgtt gattactcag ccaggttgag
    29521 agaatggcac ctaaaccgcc actttgcagt ttttccagca gcacctgaat gccgccctgc
    29581 tcctcaaccc aacttaaaat cgcctgatat ttccccgcat cgcctttcag aaaggcgccg
    29641 acaacgtcat caaaaaggcc catgataatc acctgtaaag cgttacgtgt tgactcaaaa
    29701 agtatagttc tgtgggatga taattgcgga ttgcaggaat aaaaagggcg gagatgatct
    29761 ccgccctttt cttatagctt cttgccggat gcggcgtgaa cgccttatcc ggcctacaaa
    29821 aacatgcaaa ttcaatatat tgcaggagtc tcgtaggcct gataagcgta gcgcatcagg
    29881 cacgctcgca ttgttaacgc cattaaatat caatattcgc cgctttcaga gcgttctctt
    29941 caataaacgc acggcgcggt tcaacggcgt cgcccataag cgtagtgaac aactggtcgg
    30001 cagcaatcgc atctttaacg gtaacgcgca gcatacgacg gctttccggg tccatggtgg
    30061 tttcccacag ctgttccggg ttcatctcgc ccagaccttt ataacgctgg atggagaggc
    30121 cgcgacggga ctctttcacc agccagtcca gcgcctgctc gaagctggct accggctgac
    30181 gacgttcgcc acgttcgata aatgcatctt cttccagcaa gccacgcagt ttctcaccca
    30241 gcgtgcagat acgacgatat tcgccgccag tgataaattc gtgatccagc ggatagtcag
    30301 tatccacacc gtgggtacgc acgcgaacaa tcggctcgaa caggttttgt tcggcattgg
    30361 tgtggacatc aaacttccac tggctgccgt gctgttcttt gtcgttcagt tcgctgacca
    30421 gcgcgttcac ccagcgggta acggtctgct catcagagag gtcggcttcc gtcagcgtcg
    30481 gctgatagat aagctctttc agcattgctt tcggataacg acgctccatg cggttgatca
    30541 ttttctgcgt cgcgttgtac tcagacacca gtttctctaa cgcttcgcca gccaatgccg
    30601 gtgcgctggc gttggtgtgc agcgttgcgc cgtccagcgc gatagagatc tggtactgat
    30661 ccatcgcttc gtcgtcttta atgtactgtt cctgcttgcc tttcttcact ttgtacagcg
    30721 gcggctgagc gatgtagacg tgaccgcgtt caacgatttc cggcatctga cgatagaaga
    30781 aggtcaacag cagcgtacga atgtgcgagc cgtcgacgtc cgcatcggtc atgatgatga
    30841 tgttgtgata acgcagtttg tccgggttgt actcgtcacg accgatacca caaccaagcg
    30901 cggtgatcag cgtcgccact tcctgagaag agagcatctt atcgaagcgc gctttctcga
    30961 cgttgaggat tttacccttc agcggcagaa tcgcctggtt cttgcggtta cgcccctgct
    31021 tcgcagagcc gcccgcggag tccccttcca ccaggtacag ttcggaaagc gccggatcgc
    31081 gttcctggca gtcggccagt ttgcccggca ggcccgctaa gtcgagcgca cctttacggc
    31141 gggtcatttc acgcgcgcga cgcgcagctt cacgggcacg ggcagcatcg ataattttgc
    31201 caaccacgat tttcgcgtcg gttgggtttt ccagcaggta ttccgccagc agttcgttca
    31261 tctgctgttc aaccgccgat ttcacctcag aagaaaccag tttgtctttg gtctgggagg
    31321 agaatttcgg gtccggcact ttcacggaaa cgaccgcaat caggccttca cgcgcatcgt
    31381 caccggtggc gctgactttg gcttttttgc tgtagccttc tttgtccatg taggcgttca
    31441 gggtacgggt catcgccgca cggaagcctg ccaggtgagt accgccgtca cgctgcggaa
    31501 tgttgttggt aaagcagtag atgttttcct ggaagccatc gttccactgc aacgccactt
    31561 cgacgccaat accgtctttc tcggtggaga agtagaagat attcgggtgg atcggcgttt
    31621 tgttcttgtt cagatattca acgaacgcct tgatgccgcc ttcatagtgg aagtggtctt
    31681 ctttgccgtc gcgcttgtcg cgcagacgaa tggaaacgcc ggagttgagg aacgacaact
    31741 cacgcagacg tttcgccaga atttcatatt cgaactcggt cacattggtg aaggtttcga
    31801 ggcttggcca gaaacgcacc atggtgccgg ttttttcagt ctcgccggta accgccagcg
    31861 gggcctgcgg tacaccgtgt tcgtagatct gacggtgaat tttaccctcg cgctggataa
    31921 ccagctccag tttttgcgac agggcgttta ctaccgaaac accaacgccg tgcagaccgc
    31981 cggacacttt ataggagtta tcgtcaaatt taccgcctgc gtgcagaacg gtcatgatca
    32041 cttccgccgc cgatacgccc tcttccgggt gaataccggt cggaatgccg cgcccgtcgt
    32101 cctgtacaga gacagagtta tcggcgtgaa tggtgacgat aatttcttta cagtgacccg
    32161 cgagcgcttc gtcgatagcg ttatctacca cctcgaatac catgtggtgc agaccggtgc
    32221 cgtcatccgt gtcgccgata tacatacccg ggcgcttacg caccgcatcc agccctttca
    32281 ggactttgat actggaggag tcataagaat tcgacatcaa cgtttctcgc tcatttatac
    32341 ttgggttaat ccgttatttt acccttttcc acggtaaaca tcttcgaatt ttcgtccgac
    32401 atgtctataa cgtgttcagc actgatcgcg ctgacaaaga cctgtgattg cgtcgctttt
    32461 aagcggctgg caagcagccc gcgacgctca tcatcaagct cagaggcaaa atcatctatc
    32521 aggtagagac accgccgccc gctttcacgg gtgaggaact ctccttgcgc cagacgtaag
    32581 gcgcacatca acagcttaag ctgcccacgc gataaggtat cttccaccgg cgcaccgtcg
    32641 gcgcgaatgc gtaagtccgc tttatgcggg ccatgcgcgg tgtaggttag ctggcgatcg
    32701 cgttcaaaat tacgttccag cacctcagca tattctgtct ctttctccca gccgcgctgg
    32761 aaagagaaag tcagagaaaa ctcagggaga aattgcttac aggtatcggc catatcggcc
    32821 gcgataccgg cgctatactc cgcgcgccag gtgctgattt gctctgccag cgggatcagt
    32881 tctttatccc acgggcgcag ctgttcgtaa cgtgtcacct ggcgcagcgc cgcattgcgc
    32941 tgcttgagca aacgcttgag attgctccag gcggtgaaaa atccgggttc gttgtgaaag
    33001 catccccagt cgaggaatgc tcttctgtat ttggggccgc cgttgagtaa agtaaaccct
    33061 tctggcatta tcaactgcat tggcatcagg tgcgccagtt ccgcgacctt atgcccgtct
    33121 gtaccgtcga tgcggacttt gctgtcgccc tgtttgtctt tggttaagcc aatcgccgtc
    33181 tcgcgctctt cgccctgtaa tcgcccgtgt agaacaaacg cctcctgctc gtggcgaatg
    33241 acgcgaccaa tctgcaaact gcgaaacgcc cgaccgtggc cgagcgtata gatggcttcc
    33301 agcacgctgg ttttgccact gccgttggca cctaccagaa agttaaagcc gggagataag
    33361 gcgagatccg cggtttcaat gttgcggaaa tcgcggatca acaagcgggt gagggacatt
    33421 acagtctcat tggcatgaca acataagccg cgctctggct ggccgcatct tcaatctgca
    33481 cgctggaaac cgaatcggtc agcatcatgc ggacgttttc gcatttcagt gcgttcagaa
    33541 catccagcac atagctgacg ttgaagccga tttccatctc cgcaccgcta taggtaacgt
    33601 cgaggatctc ttccgcttct tcctgttctg ggttgttggc ggtgattttc agctggtttt
    33661 cgctgacata cagacgcacg ccgcggaatt tctcgttaga gagaattgcc gcacgggcaa
    33721 acgcctgctt gagcagatca cagccagctt ccagatgctt gtccgggttc ttcggcagaa
    33781 cgcgacggta atccgggaag cgaccatcca ccagtttcga ggtgaagata aagtcgccaa
    33841 cgtgggcgcg aatgttgttg ctgccaatct gcacgcgcag cggattgtcg ccgccgtcga
    33901 gcatacgcat cagttcaatc acgcctttac gcggcacgat caccgaatgg cttggcaaag
    33961 attgaccaat tggcattgaa cagaccgcca gacggtggcc gtcggtcgcc acggtgcgca
    34021 gttcttcacc ttcggtttca aacagcatac cgtttaagta atagcgaacg tcctgatgtg
    34081 ccatagaaaa ctgggtcgct tcaatcagac gcttcatcgt cgcctgcggc agggtaaatt
    34141 cgacttcgct ctgccagtca tcgaggttcg ggaaatctgc cgctggcagg gtagacagcg
    34201 aaaaacggct acgcccggag cgcaccagca tccgctcacc ttccagctgc acggcaattt
    34261 ccgcgccttc aggcagacca cggcagatat caaagaattt gcgcgccgga acggtcgtcg
    34321 cccctggttc gtgcggctga accagcgcaa cgcgtgccac catttccatc tcgagatcag
    34381 taccggtcag cgacaacgta ccgtcagcaa cctgtaacag cagattaccg agaatcggta
    34441 gcgtaggacg accacctaac ggaccgctca cctgttgtag cggttttaat aaatgctcac
    34501 gttctacggt aaatttcata ggtttacgat gacaatgttc tgattaaatt agagaaatct
    34561 tctttgatat cgtggctctc ttcacgcaac tgctcgatct tccggcaggc atgaagcacc
    34621 gtcgtgtggt cacgaccacc aaacgcatcg ccaatctccg gcagactgtg gttagtcagc
    34681 tctttcgcca gcgccatcgc catctggcgc ggacgtgcca ccgagcggga tcgacgcttg
    34741 gaaaggagat ccgcgacttt gatcttgtag tactccgcca ccgtcttctg aatattgtcg
    34801 atggtgacca gtttttcctg caatgccagc aagtcgcgca gcgcctcacg cacgaagtcg
    34861 atggtgatcg cccgtccggt aaagttggcg ttagcaataa cgcggttcag cgcgccttcc
    34921 agctcacgta cgttagatcg tagacgcttg gcgataaaga acgccacttc gcccggcaaa
    34981 cgaatgtcgt tttcgtcggc ctttttcatc aggatcgcca cgcgggtttc taactctggc
    35041 ggttcgatcg ccacagtcag tccccaaccg aagcgggatt tcagacgatc ctcaacgcca
    35101 ttgatctctt tcggatagcg atccgaggtg agaatgatct gctggttacc ttccaacagg
    35161 gcgttgaagg tgtggaaaaa ctcttcctga gatcgttctt tattagcaaa aaactgaata
    35221 tcgtcgatca gcagtgcatc tacggaacgg tagtagcgtt taaactcttc gatcgcgttg
    35281 ttttgcaggg ctttaaccat gtcctgaaca aagcgctcgg agtgcatata aaccactttg
    35341 gcattcggct tgcgcgccat aatgccgtta cccaccgcat gcagcaggtg agttttaccc
    35401 agacccgtgc cgccataaag gaacaacggg ttataggcac cgccagggtt atctgccacc
    35461 tggcgagccg ccgcgcgcgc cagttggtta gatttacctt caacgaagtt atcaaacgtg
    35521 tgtttgacgt ttacgttaga acgataggtc ggttctgccg gggccgggac gttatcccaa
    35581 cctgagcgcg tagaaggcgc agcacgttgc ggctgcgttt gcgccacctg tgcaggggcc
    35641 gcgacgttgc tcgtcactgc cgcttgtggc gtttgcgtca ccggtttggt gccgacttca
    35701 aaacgcagct gtggggcatc cgctccgcag aaactggtta gcagtccatt gatattatta
    35761 aggtacttgt cccgtaccca atcgaggaca aaacggtttg gcgcgtacag ggccagcgtg
    35821 ttatcgctca gttccgcctg caatgggcgt atccacatac tgaattctgt ggctggtaac
    35881 tcatcctgca atcgggcaag acactgctgc caaagcgaaa gtgacacggc ggactccact
    35941 cgaacaaaag tcgataatga ctaaggctga aacattcatg attgttgacg tacgtcgaaa
    36001 agaccctgca tgagggtgac gcacgaaccg ctgtctgcgg ttatatgccc gatcaagatc
    36061 ctgcaaaacg atcgggaccg cggatcatag cctaaactgc gcaagagatc ttctgtttct
    36121 cacagatttt cccgatttat ccacaggact ttccagaact cgctaagtgt aaacgatcct
    36181 gccgcagggc gggcacgatt tacgccgcat attggaaaat ttaatgacca tagacaaaaa
    36241 ttggcttaat cgatctaata aagatccagg acgatccttg cgctttaccc gtcagcccgt
    36301 ataatcctcc acccggcgcg ccatgctggt tttcactggt gtgaggccgt acgttttccc
    36361 tgcgaaaagg tgcggaaaag cgcggtaaat aaggaaagag aattgactcc ggagtgtaca
    36421 attattacaa tccggcctct ttaatcaccc atggcttcgg tgtccatcgt ttcatttttc
    36481 ggcggatatc caataaagcc attgaattta ttcaagttta ggtagaaatc gccatgaaac
    36541 gcacttttca accgtctgta ctgaagcgca accgttctca cggcttccgt gctcgtatgg
    36601 ctactaaaaa tggtcgtcag gttctggcac gtcgtcgtgc taaaggccgc gctcgtctga
    36661 ccgtttctaa gtaataaagc taacccctga gtggttaagc tcgcatttcc tagggagtta
    36721 cgcttgttaa ctcccagtca attcacattc gtcttccagc agccacaacg ggctggcacg
    36781 ccgcaaatta ccattctcgg ccgcctgaat tcgctggggc atccccgtat cggtcttaca
    36841 gtcgccaaga aaaacgttcg acgcgcccat gaacgcaatc ggattaaacg tctgacgcgt
    36901 gaaagcttcc gtctgcgcca acatgaactc ccggctatgg atttcgtggt ggtggcgaaa
    36961 aaaggggttg ccgacctcga taaccgtgct ctctcggaag cgttggaaaa attatggcgc
    37021 cgccactgtc gcctggctcg cgggtcctga tagccctcat tcgggtctat caacgcctga
    37081 ttagtccgct actcgggccg cattgtcgtt tcactccaac ctgttcaagc tacggaattg
    37141 aggcattgcg caggtttgga gtgataaaag gcagttggtt gacggtgaaa cgcgtattaa
    37201 aatgccaccc tttacaccct ggtggtgacg atcccgtccc gcccggacca tttgatacca
    37261 gagaacacta acgatggatt cgcaacgcaa tcttttagtc atcgctttgc tgttcgtgtc
    37321 tttcatgatc tggcaagcct gggagcagga taaaaacccg caacctcagg cccaacagac
    37381 cacgcagaca acgaccaccg cagcgggtag cgccgccgac cagggcgtac cggccagtgg
    37441 ccaggggaaa ctgatctcgg ttaagaccga cgtgcttgat ctgaccatca acacccgtgg
    37501 tggtgatgtt gagcaagctc tgctgcctgc ttacccgaaa gagctgaact ctacccagcc
    37561 gttccagctg ctggaaactt caccgcagtt tatttatcag gcacagagcg gtctgaccgg
    37621 tcgtgatggc ccggataacc cggctaacgg cccgcgtccg ctgtataacg ttgaaaaaga
    37681 cgcttatgtg ctggctgaag gtcaaaacga actgcaggtg ccgatgacgt ataccgacgc
    37741 ggcaggtaac acgtttacca aaacgtttgt cctgaaacgt ggtgattacg ctgtcaacgt
    37801 caactacaac gtgcagaacg ctggcgagaa accgctggaa atctccacct ttggtcagtt
    37861 gaagcaatcc atcactctgc caccgcatct cgataccgga agcagcaact tcgcactgca
    37921 caccttccgc ggcgcggcgt actccacgcc tgacgagaag tacgagaaat acaagttcga
    37981 taccattgcc gataacgaaa acctgaacat ctcttcgaaa ggtggttggg tggcaatgct
    38041 gcaacagtat ttcgcgacgg cgtggatccc gcataacgac ggtaccaaca acttctatac
    38101 cgctaatctg ggtaacggca tcgccgctat cggctataaa tctcagccgg tactggttca
    38161 gcctggtcag actggcgcga tgaacagcac cctgtgggtt ggcccggaaa ttcaggacaa
    38221 aatggcagct gttgctccgc acctggatct gaccgttgat tacggttggt tgtggttcat
    38281 ctctcagccg ctgttcaaac tgctgaaatg gatccatagc tttgtgggta actggggctt
    38341 ctccattatc atcatcacct ttatcgttcg tggcatcatg tacccgctga ccaaagcgca
    38401 gtacacctcc atggcgaaga tgcgtatgct gcagccgaag attcaggcaa tgcgtgagcg
    38461 tctgggcgat gacaaacagc gtatcagcca ggaaatgatg gcgctgtaca aagctgagaa
    38521 ggttaacccg ctgggcggct gcttcccact gctgatccag atgccaatct tcctggcgtt
    38581 gtactacatg ctgatgggtt ccgttgaact gcgtcaggca ccgtttgcac tgtggatcca
    38641 cgacctgtct gcacaggacc cgtactacat cctgccgatc ctgatgggcg taacgatgtt
    38701 cttcattcag aagatgtcgc cgaccactgt gaccgacccg atgcagcaga agatcatgac
    38761 ctttatgccg gtcatcttca ctgtgttctt cctgtggttc ccgtcaggtc tggtgctgta
    38821 ctatatcgtc agcaacctgg taaccattat tcagcagcag ctgatttacc gtggtctgga
    38881 aaaacgtggc ctgcatagcc gcgagaagaa aaaatcctga ttcggtgagt tttcgctaaa
    38941 ataagggcgg tcagttgacc gccttttttc ttttcgtagg gcggataagc accgcacatc
    39001 cgccacacaa agcaacagga acatcatgag cgataatgac actatcgtag cccaggccac
    39061 gcctccggga cgtggcggcg ttggcatcct gcgcatctcc ggcttcaaag cccgtgaagt
    39121 tgccgaaacc gtgctgggta aactgcctaa gccgcgctac gccgattatc ttccgtttaa
    39181 agacgccgac ggcagcgtgc tcgatcaggg gattgcgcta tggttccctg gcccgaactc
    39241 gttcaccggc gaagatgtgc tggaactgca aggtcatggc ggtccggtga tcctcgacct
    39301 gctgttaaaa cgcattctga ccattcccgg cctgcggatt gctcgccctg gtgagttttc
    39361 cgaacgcgcg tttcttaacg ataaactcga cttagcccag gccgaggcga ttgcggatct
    39421 tatcgacgcc agttcggaac aggcggcccg ttcggcactt aactcgctgc aaggcgcatt
    39481 ctccgcacgg gttaatcatc tggtagaagc cctcacccac ttgcgcattt acgttgaagc
    39541 ggcaattgat ttccccgatg aagagatcga tttcctctcc gacggcaaaa ttgaagccca
    39601 gcttaatgac gtgattgccg atctcgatgc agtgcgtgct gaagcacgtc agggtagttt
    39661 gttgcgcgaa gggatgaaag tggtgattgc cggacgtcct aacgccggta aatcgagcct
    39721 gttaaacgcg ctggctggcc gtgaagcagc aatcgtaacc gatatcgccg gaactacgcg
    39781 tgacgtgttg cgtgagcata tccacattga cggaatgccg ctgcatatca tcgacaccgc
    39841 cgggctacgt gaagccagtg acgaagttga acgtattggt atcgagcgcg catggcagga
    39901 aattgaacag gcagaccgcg tgctgtttat ggtcgatggc accacgacag acgccgttga
    39961 tccggcagag atctggccgg aatttatcgc ccgtctgcca gcgaaactgc cgatcaccgt
    40021 ggtgcgcaat aaagccgata tcaccggcga aacgttgggg atgagcgaag tgaacggtca
    40081 cgcgttaatt cgtctttcgg cgcggactgg cgaaggcgtg gatgtgctgc gtaaccatct
    40141 caaacagagc atgggctttg acaccaatat ggaaggcggc ttcctggcgc gtcgtcgtca
    40201 cctacaggca ctggaacagg cggcagagca cctgcaacag ggtaaagcgc aactgttagg
    40261 agcctgggca ggcgagctgc tggcggaaga actgcgtctg gcgcagcaga acttaagcga
    40321 gatcaccggg gaatttactt ctgacgacct gctggggcga attttctcca gcttctgtat
    40381 tggtaagtaa ccgcgcttgc gaagccgcat tctgactgtc agatgcggct tcgcttcatt
    40441 gttagcactc ctgttattcc tcaacccttt tttaaacatt aaaattctta cgta
//
LOCUS       DAPHCV010000032        39945 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.032, whole genome shotgun sequence.
ACCESSION   DAPHCV010000032 DAPHCV010000000
VERSION     DAPHCV010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.032"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            331..417
                     /gene="leuL"
                     /locus_tag="RH033_001717"
     CDS             331..417
                     /gene="leuL"
                     /locus_tag="RH033_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HDV2990913.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            510..2081
                     /gene="leuA"
                     /locus_tag="RH033_001718"
     CDS             510..2081
                     /gene="leuA"
                     /locus_tag="RH033_001718"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDV2990914.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2081..3172
                     /gene="leuB"
                     /locus_tag="RH033_001719"
     CDS             2081..3172
                     /gene="leuB"
                     /locus_tag="RH033_001719"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDV2990915.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLSPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTNEMGDIIARYVVEGV"
     gene            3175..4575
                     /gene="leuC"
                     /locus_tag="RH033_001720"
     CDS             3175..4575
                     /gene="leuC"
                     /locus_tag="RH033_001720"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDV2990916.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4586..5191
                     /gene="leuD"
                     /locus_tag="RH033_001721"
     CDS             4586..5191
                     /gene="leuD"
                     /locus_tag="RH033_001721"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDV2990917.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVQANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEEKQPAFMR"
     gene            complement(5299..5430)
                     /gene="sgrT"
                     /locus_tag="RH033_001722"
     CDS             complement(5299..5430)
                     /gene="sgrT"
                     /locus_tag="RH033_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HDV2990918.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            5519..7174
                     /gene="sgrR"
                     /locus_tag="RH033_001723"
     CDS             5519..7174
                     /gene="sgrR"
                     /locus_tag="RH033_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HDV2990919.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAA
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQILPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     SSNQLKIQAFDDFFGYRALIDEVNVWVLPEIADDPAGGLMLKGPQDEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWISYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHTRT
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEINLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      7208..7307
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7338..8321
                     /gene="thiB"
                     /locus_tag="RH033_001724"
     CDS             7338..8321
                     /gene="thiB"
                     /locus_tag="RH033_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HDV2990920.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWKNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            8297..9907
                     /gene="thiP"
                     /locus_tag="RH033_001725"
     CDS             8297..9907
                     /gene="thiP"
                     /locus_tag="RH033_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HDV2990921.1"
                     /translation="MATRRQPLIPGWLIPGISAATLVVAVALAAFLALWWNAPQGDWV
                     AIWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPTRAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVLVDGINRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            9891..10589
                     /gene="thiQ"
                     /locus_tag="RH033_001726"
     CDS             9891..10589
                     /gene="thiQ"
                     /locus_tag="RH033_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HDV2990922.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERSEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKITLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGIKG"
     gene            complement(10703..11467)
                     /locus_tag="RH033_001727"
     CDS             complement(10703..11467)
                     /locus_tag="RH033_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV2990923.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(11553..12431)
                     /gene="araC"
                     /locus_tag="RH033_001728"
     CDS             complement(11553..12431)
                     /gene="araC"
                     /locus_tag="RH033_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HDV2990924.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            12770..14470
                     /gene="araB"
                     /locus_tag="RH033_001729"
     CDS             12770..14470
                     /gene="araB"
                     /locus_tag="RH033_001729"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HDV2990925.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHTPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQEIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIANKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            14481..15983
                     /gene="araA"
                     /locus_tag="RH033_001730"
     CDS             14481..15983
                     /gene="araA"
                     /locus_tag="RH033_001730"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDV2990926.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            16268..16963
                     /gene="araD"
                     /locus_tag="RH033_001731"
     CDS             16268..16963
                     /gene="araD"
                     /locus_tag="RH033_001731"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDV2990927.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRGRGVLVIKPS
                     GVDYSIMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            17038..19389
                     /gene="polB"
                     /locus_tag="RH033_001732"
     CDS             17038..19389
                     /gene="polB"
                     /locus_tag="RH033_001732"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HDV2990928.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWMATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGSIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            19553..22459
                     /gene="rapA"
                     /locus_tag="RH033_001733"
     CDS             19553..22459
                     /gene="rapA"
                     /locus_tag="RH033_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HDV2990929.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDETEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            22471..23130
                     /gene="rluA"
                     /locus_tag="RH033_001734"
     CDS             22471..23130
                     /gene="rluA"
                     /locus_tag="RH033_001734"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HDV2990930.1"
                     /translation="MGMENYNPPQEPWLVVLYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(23248..24063)
                     /gene="djlA"
                     /locus_tag="RH033_001735"
     CDS             complement(23248..24063)
                     /gene="djlA"
                     /locus_tag="RH033_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HDV2990931.1"
                     /translation="MQYWGKIIGVAVALIMGVGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            24318..26672
                     /gene="lptD"
                     /locus_tag="RH033_001736"
     CDS             24318..26672
                     /gene="lptD"
                     /locus_tag="RH033_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HDV2990932.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWKHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSIGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTRLAESANRVMPQFKVDGRMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSKIYNYDSSLLQSDYSGLFRDRTYGGLDRIASTNQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISDRWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            26725..28011
                     /gene="surA"
                     /locus_tag="RH033_001737"
     CDS             26725..28011
                     /gene="surA"
                     /locus_tag="RH033_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HDV2990933.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            28011..29000
                     /gene="pdxA"
                     /locus_tag="RH033_001738"
     CDS             28011..29000
                     /gene="pdxA"
                     /locus_tag="RH033_001738"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HDV2990934.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDKLRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ"
     gene            28997..29818
                     /gene="rsmA"
                     /locus_tag="RH033_001739"
     CDS             28997..29818
                     /gene="rsmA"
                     /locus_tag="RH033_001739"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDV2990935.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDKLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            29821..30198
                     /gene="apaG"
                     /locus_tag="RH033_001740"
     CDS             29821..30198
                     /gene="apaG"
                     /locus_tag="RH033_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HDV2990936.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            30205..31053
                     /gene="apaH"
                     /locus_tag="RH033_001741"
     CDS             30205..31053
                     /gene="apaH"
                     /locus_tag="RH033_001741"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HDV2990937.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDMGE
                     GEAAAS"
     gene            complement(31050..31166)
                     /locus_tag="RH033_001742"
     CDS             complement(31050..31166)
                     /locus_tag="RH033_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HDV2990938.1"
                     /translation="MTHETATVPVNALGTKFCDASAHRTLIKGALDFMLDGI"
     gene            complement(31163..31351)
                     /locus_tag="RH033_001743"
     CDS             complement(31163..31351)
                     /locus_tag="RH033_001743"
                     /inference="COORDINATES: protein motif:HMM:NF013965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HDV2990939.1"
                     /translation="MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFI
                     RIRPPERLNTILLLVDGS"
     gene            complement(31354..31587)
                     /locus_tag="RH033_001744"
     CDS             complement(31354..31587)
                     /locus_tag="RH033_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HDV2990940.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AAIRWREENAEAIAALNQLADETGCFSDEYRSF"
     gene            complement(31673..32152)
                     /gene="folA"
                     /locus_tag="RH033_001745"
     CDS             complement(31673..32152)
                     /gene="folA"
                     /locus_tag="RH033_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HDV2990941.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(32344..34206)
                     /gene="kefC"
                     /locus_tag="RH033_001746"
     CDS             complement(32344..34206)
                     /gene="kefC"
                     /locus_tag="RH033_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HDV2990942.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSSEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(34199..34729)
                     /gene="kefF"
                     /locus_tag="RH033_001747"
     CDS             complement(34199..34729)
                     /gene="kefF"
                     /locus_tag="RH033_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HDV2990943.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(34837..36168)
                     /locus_tag="RH033_001748"
     CDS             complement(34837..36168)
                     /locus_tag="RH033_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2990944.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(36227..36514)
                     /gene="fixX"
                     /locus_tag="RH033_001749"
     CDS             complement(36227..36514)
                     /gene="fixX"
                     /locus_tag="RH033_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HDV2990945.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(36511..37797)
                     /locus_tag="RH033_001750"
     CDS             complement(36511..37797)
                     /locus_tag="RH033_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDV2990946.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGLINLIKDGMKGVTVL"
     gene            complement(37848..38789)
                     /locus_tag="RH033_001751"
     CDS             complement(37848..38789)
                     /locus_tag="RH033_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDV2990947.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLSGKPDERMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LNAAVSNDASAVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDVAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(38804..39574)
                     /locus_tag="RH033_001752"
     CDS             complement(38804..39574)
                     /locus_tag="RH033_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HDV2990948.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     VLAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         9577 a        10961 c        10551 g         8856 t
ORIGIN      
        1 tcaataatat tcaccaaatg aatatcaaaa aattcgcaaa acatataatt caatacaaat
       61 catcaggata ggttttgtaa cgcgtgcatt ttatccccct ttccctcgtt gattagatgc
      121 aaaattttat tctgaaatat gtcaaccgat gaaaagcgtc ggtagttaag cagaaattaa
      181 tatcgcttac tttaaccacc gcagcacaat tagctaattt tacggatgca gaactcatgc
      241 tggcgggacg tttttattgc gtcagggttg acatccgttt ttgtatccag taactctaaa
      301 agcatatcgc attcatctgg agctgattta atgactcaca tcgttcgctt tatcggtcta
      361 ctactactaa acgcatcttc tttgcgcggt agacgagtga gcggcatcca gcattaagcc
      421 agcacgcagt caaacaaaaa acccgcgcca ttgcgcgggt ttttttatgc ccgaagcgag
      481 gcgctcaaaa agagacaagg acccaaacca tgagccagca agtcattatt ttcgatacca
      541 cattgcgcga cggtgaacag gcgttacagg caagcttgag tgtgaaagaa aaactgcaaa
      601 ttgcgctggc ccttgagcgt atgggtgttg acgtgatgga agtcggtttc cccgtctctt
      661 cgccgggcga ttttgaatcg gtgcaaacca tcgctcgcca ggtcaaaaac agccgcgtat
      721 gtgcgttagc tcgctgcgtg gagaaagata ttgacgtggc ggccgaatct ctgaaagtcg
      781 ccgaagcctt ccggattcat acctttattg ccacttcgcc aatgcacatt gccaccaaac
      841 tgcgcagcac gctggacgag gtgatcgaac gcgctatcta tatggtgaaa cgcgcccgta
      901 attacaccga tgatgttgaa ttttcttgcg aagatgccgg acgcacccca attgccgatc
      961 tggcgcgagt ggtcgaagcg gcgattaatg ccggtgccac caccatcaac attccggaca
     1021 ccgtgggcta caccatgcca tttgagttcg ccggaatcat cagcgggctg tatgaacgcg
     1081 tacccaacat cgacaaagcc attatttccg tacataccca cgacgatctg ggcctggcgg
     1141 tcggcaactc actggcggcg gtacatgccg gtgcacgtca ggtggaaggt gcaatgaacg
     1201 ggatcggcga gcgtgccggt aactgttccc tggaagaagt catcatggcg attaaagttc
     1261 gtaaggatat tctcaacgtc cacaccgcca ttaatcacca ggaaatatgg cgcaccagcc
     1321 agttagttag ccagatttgt aatatgccga tcccggcaaa caaagccatt gttggcagcg
     1381 gcgcattcgc acactcctcc ggtatccacc aggatggcgt gctgaaaaac cgcgaaaact
     1441 acgaaatcat gacaccagaa tctattggtc tgaaccaaat ccagctgaat ctgacctctc
     1501 gttcggggcg tgcggcggtg aaacatcgca tggatgagat ggggtataaa gaaagtgaat
     1561 ataatttaga caatttgtac gatgctttcc tgaagctggc ggacaaaaaa ggtcaggtgt
     1621 ttgattacga tctggaggcg ctggccttca tcggtaagca gcaagaagag ccagagcatt
     1681 tccgtctgga ttacttcagc gtgcagtctg gctctaacga tattgccact gccgccgtca
     1741 aactggcctg cggcgaagaa gtcaaagcag aagccgccaa cggtaacggt ccggtcgatg
     1801 ccgtctatca ggcaattaac cgcatcactg actataacgt cgaactggtg aaatacagcc
     1861 tgaccgccaa aggccacggt aaagatgcgc tgggtcaggt ggatatcgtc gcaaattaca
     1921 acggtcgccg cttccacggc gtcggcctgg ccaccgatat tgtcgagtcc tccgccaaag
     1981 ccatggtgca cgtactgaac aatatctggc gtgccgcaga agtcgaaaaa gagttgcaac
     2041 gcaaagctca acacaacgaa aacaacaagg aaaccgtgtg atgtcgaaga attaccatat
     2101 tgccgtattg ccgggggacg gtattggtcc ggaagtgatg acccaggcgc tgaaagtgct
     2161 ggatgccgtg cgcaaccgct ttgcgatgcg tatcaccacc agccattacg atgtaggcgg
     2221 cgcagccatt gataaccacg ggcagccgct gccgcctgcg acggttgaag gttgtgagca
     2281 agccgatgcc gtgctgtttg gctcggtagg tggtccgaag tgggaacatt tgtcgccaga
     2341 ccagcaacca gaacgcggcg cactgttgcc tttgcgtaaa cacttcaaat tattcagcaa
     2401 tctgcgtccg gcaaaactgt atcaggggct ggaagcattc tgtccgctgc gtgctgacat
     2461 tgccgctaac ggcttcgaca tcctgtgtgt gcgcgaactg accggcggca tctatttcgg
     2521 tcagccaaaa ggccgcgaag gtagcggaca atatgaaaaa gcgtttgata ccgaggtgta
     2581 tcaccgtttt gagattgaac gtatcgcccg catcgcgttt gaatctgctc gtaaacgtcg
     2641 ccataaagtg acgtctatcg acaaagccaa cgtgctgcaa tcctctattt tatggcggga
     2701 gatcgttaac gagatcgcca cggaataccc ggatgtcgaa ctggcgcata tgtacatcga
     2761 caacgccacc atgcagctga ttaaagatcc atcacagttt gacgtcctgc tgtgttccaa
     2821 cctgtttggc gacattctgt ctgacgagtg cgcaatgatc actggctcga tggggatgtt
     2881 gccttccgcc agcctgaacg agcaaggttt tggtttgtat gaaccggcag gcggctcagc
     2941 accagatatc gcaggcaaaa acatcgccaa cccgattgca caaatcctgt cgctggcact
     3001 gctgctgcgt tacagcctgg atgccgatga tgcggcttgc gccattgaac gcgccattaa
     3061 ccgcgcatta gaagaaggca ttcgcaccgg agatttagcc cgtggcgctg ccgccgttag
     3121 taccaatgaa atgggcgata tcattgcccg ctatgtggta gaaggggtgt aatcatggct
     3181 aaaacgttat acgaaaaatt gttcgacgct cacgtagtgt acgaagccga aaacgaaacc
     3241 ccgctgttat atatcgaccg ccacctggtg catgaagtga cctcaccgca ggcgttcgat
     3301 ggtctgcgcg cccacggtcg cccggtacgt cagccaggca aaaccttcgc cactatggat
     3361 cacaacgtct ctactcagac caaagacatt aatgcctgcg gtgaaatggc gcgcatccag
     3421 atgcaggagc tgatcaaaaa ctgcaaagaa tttggcgtcg agctgtatga cctgaatcac
     3481 ccgtatcagg ggatcgtcca cgtaatgggg ccggaacagg gcgtcacatt gccggggatg
     3541 accattgtct gcggcgactc gcataccgcc acccacggcg cgtttggcgc actagccttt
     3601 ggtatcggca cttccgaagt tgaacacgta ctggcaacgc aaaccctgaa acagggtcgt
     3661 gcgaagacca tgaaaattga agtccagggc aaagccgcgc cgggcattac agcgaaagat
     3721 atcgtgctgg cgattatcgg taaaactggt agcgcaggcg gcaccgggca tgtggtggag
     3781 ttttgcggcg aagcaatccg tgatttaagc atggaaggtc gtatgaccct gtgcaatatg
     3841 gcaatcgaaa tgggcgcgaa agccggtctg gttgcaccgg acgaaactac ctttaactat
     3901 gtcaaaggcc gtctgcatgc gccgaaaggc aaagatttcg acgacgccgt tgcctactgg
     3961 aaaactctgc aaaccgacga aggcgcaact ttcgataccg ttgtgacttt gcaggcagaa
     4021 gaaatttcac cgcaggtcac ctggggcacc aatccaggtc aggtgatttc cgtgaacgac
     4081 aacattcccg atccggcttc gtttgccgat ccggttgaac gcgcgtcggc agaaaaagcg
     4141 ctggcctata tggggctgaa accgggtatt ccgctgacag aagtggctat cgacaaagtg
     4201 tttatcggtt cctgcactaa ctcgcgtatt gaagatttac gcgcggcagc tgaaatcgcc
     4261 aaaggacgga aagtcgcgcc aggcgtgcag gcactggtgg ttcccggctc tggcccggta
     4321 aaagcccagg cggaagcgga aggtctggac aaaatcttta ttgaagccgg ttttgaatgg
     4381 cgcttgccag gttgctcaat gtgtctggcg atgaacaacg atcgcctgaa tccgggtgag
     4441 cgttgtgcct ccaccagcaa ccgtaacttt gaaggccgcc aggggcgtgg agggcgcacc
     4501 catctggtca gcccggcaat ggctgccgct gctgctgtga ccggacattt cgccgacatt
     4561 cgcaacatta aataaggagc acaccatggc agagaaattt atcaaacaca caggcctggt
     4621 ggttccgctg gatgccgcca atgtcgatac cgatgcaatc atcccgaaac agtttttgca
     4681 gaaagtgacc cgtacgggtt ttggcgcgca tctgtttaac gactggcgtt ttctggatga
     4741 aaaaggccaa cagccaaacc cggacttcgt gctgaacttc ccgcagtatc agggcgcttc
     4801 cattttgctg gcacgagaaa acttcggctg cggttcctca cgtgagcacg cgccgtgggc
     4861 attgaccgat tacggtttta aagtggtgat tgcaccgagt tttgctgaca ttttctacgg
     4921 caacagcttt aacaaccagc tgctgccggt gaaattaagc gacgcagaag tggacgaact
     4981 gtttgcgctg gtgcaggcca atccggggat tcattttgat gtagacctgg aagcgcaaga
     5041 ggtgaaagcg ggagagaaaa cttatcgctt taccatcgat gccttccgcc gccactgcat
     5101 gatgaacggt ctggacagca tcggcctgac tctgcagcac gacgacgcaa ttgcgtctta
     5161 tgaagagaag caaccggcgt ttatgcgtta gtttgtacac cgtccggcac tcaatcgcca
     5221 aaaagaaaaa agccagcaga taatcactgc tggcgtggcg attaacacca atactcagtc
     5281 acacatgatg tccgcaaatc agtagtcaga atgactcccc tcccattgca tcagttcttc
     5341 aagcatggtt aatcgttgtg ggacgcttaa ccaacgcaac cagcacaact tcgctgtcgc
     5401 ggtaaaatag tgctgataaa actgacgcat gaggcacctc cttgcttcat cgttaggaat
     5461 tattgactta atatagggaa aataaaattg ctgtcttttg cgcaggagtt ccccttttat
     5521 gccatctgct cgtctgcaac aacagttcat ccgcctgtgg caatgctgcg agggtaaatc
     5581 gcaggacacg acgctcaacg aactggcagc gttattgagc tgctcgcgtc gtcatatgcg
     5641 caccctgctc aacaccatgc aggatcgcgg ctggctgacg tgggaagcgg aagtcgggcg
     5701 cggcaaacgc tcgcgtctga cattcctcta taccgggctg gcgcttcagc aacagcgagc
     5761 agaggacctg ctggagcagg atcgtatcga tcaactggtg caactggttg gcgacaaagc
     5821 ggctgtgcgg caaatgttgg tttctcatct gggacgcagc ttccgccagg ggcggcacat
     5881 cctgcgcgtt ctctactatc gtccgttgcg taatctgctg cccggcagtg cattgcgtcg
     5941 ttccgaaacc catatcgccc ggcaaatctt cagttcgcta acgcgcataa atgaggaaaa
     6001 tggggaactg gaagcggaca tcgcccacca ctggcagcaa attttaccgc ttcactggcg
     6061 tttctttttg cgtccaggag tccattttca tcatggtcgt gaactggaaa tggacgacgt
     6121 gatcgcctct ttaaaacgaa tcaatacgct gccgctctat tcgcatattg ctgacattgt
     6181 gtcgccgacg ccctggacgc tggatatcca tctcacgcaa ccggaccgct ggttaccgtt
     6241 actgctgggg caagttccgg cgatgatcct gccgcgcgaa tgggaaaccc tcagcaactt
     6301 tgccagccat cccatcggaa cgggtcctta tgcggtgatc cgcaacagca gcaatcaact
     6361 gaaaattcag gcattcgacg acttctttgg ttatcgggca ttaatcgacg aagttaacgt
     6421 ctgggtcttg ccggaaattg ctgatgaccc cgcgggaggc ctgatgctga aaggaccgca
     6481 agacgaggaa aaagagattg aaagccgcct tgaagaaggt tgttactatt tactgttcga
     6541 cagccgtacc catcgcgggg cgaatcagca agtcagagac tggataagct atgtgctttc
     6601 gccgaccaat ctggtttatt tcgccgagga acagtaccag cagttgtggt ttccggccta
     6661 tgggctgctt ccccgttggc accatacccg caccataaag agcgaaaaac cggctggtct
     6721 ggaaagcctc accctgacct tttatcagga tcacagtgag catagggtga ttgccgggat
     6781 catgcagcag attctggcaa gtcaccaggt cacgctggaa atcaaagaga tcagctacga
     6841 tcagtggcat gaaggagaga tcgagagcga tatctggctt aacagcgcca actttacgct
     6901 gccgctggat ttttcgctat tcgcgcacct gtgcgaggtg ccgctgctac aacactgtat
     6961 tccgatcgac tggcaagccg acgccgcccg ctggcgcaat ggcgaaataa acctggcgaa
     7021 ctggtgccag caactggtcg ccagcaaagc aatggtgccg cttatccacc actggctgat
     7081 cattcagggg caacgcagta tgcgcggcct gcgtatgaat accctcggct ggttcgattt
     7141 taaatcagcg tggtttgcgc cgccggatcc atgattgctg gttgataaca aaatcactac
     7201 actaacgccg ttctcaacgg ggtgccacgc agacgcgtgc gctgagaaaa tacccgtcga
     7261 acctgatccg gataacgccg gcgaagggat ttgaggctcc ttctcaagtc ctttgccact
     7321 cttttttgag gtgcaaagtg ttaaaaaaat gtctcccctt gctgttgctg tgcacagcgc
     7381 ccgttttcgc taaacccgtt ctgactgttt atacctacga ttccttcgcc gccgactggg
     7441 ggcctggtcc aaagatcaaa aaagcttttg aagccgactg taactgcgaa ctgaaactgg
     7501 tggcgctgga agatggcgtt tcgctgctca accgtctgcg gatggaaggc aaaaacagta
     7561 aagccgatgt ggtgctgggg ctggataaca acctgttaga cgccgccagt aaaaccggac
     7621 tatttgccaa aagcggcgtg gcagcggatg ccgttaacgt tcccggcggc tggaaaaatg
     7681 acactttcgt accgtttgat tacggctatt tcgccttcgt ctatgacaag aacaaactga
     7741 aaaatccgcc gcaaagcctg aaagagcttg ttgagagcga tcaaaactgg cgggtgattt
     7801 atcaggatcc gcgcaccagt acgccggggc tgggcctgct gttgtggatg caaaaagtct
     7861 atggcgatga cgccccacaa gcctggcaga aactggcgaa gaaaacggtc acggtcacca
     7921 aaggctggag cgaagcctac ggcctgtttt taaaaggtga aagcgatctg gtactgagtt
     7981 acaccacctc tccggcttat cacattctcg aagagaagaa agataactac gccgccgcaa
     8041 acttcagcga aggtcactat ctgcaggtgg aagtcgccgc ccgcaccgct gccagcaagc
     8101 agccggagct ggcgcaaaaa tttctccagt ttatggtttc tccggctttt cagaatgcga
     8161 tcccaaccgg caactggatg tatccggtgg caaacgtcac gctgcctgcc ggttttgaac
     8221 aattgaccaa accggcaacc acgctggagt tcacgccagc cgaagtggcg gcacaacgtc
     8281 aggcatggat tagcgaatgg caacgcgccg tcagccgtta attcccggct ggttaattcc
     8341 aggaataagc gccgccacgc tggtggtggc ggttgcactg gctgcgtttc tcgccctgtg
     8401 gtggaacgcg ccgcagggtg actgggtggc aatctggcag gacagctacc tgtggcatgt
     8461 ggtgcgcttc tccttctggc aggcgtttct ctcggcgctg ctctctgtcg tgcccgcgat
     8521 attccttgcc cgcgccctct accgcaggcg ctttccagga cggctggcgc tgttgcgtct
     8581 gtgtgccatg accttgatcc tcccggtgct ggttgctgtt ttcggcattc ttagcgtcta
     8641 tggtcgccag ggctggctgg catcgctctg ccaatcgctc ggtctggagt ggaccttttc
     8701 gccctacggc ctgcaaggca ttttgctggc ccatgtgttt tttaatctgc cgatggcgag
     8761 ccgtttatta cttcaggcac tggaaaacat tcccggcgaa caacgtcaac ttgccgccca
     8821 gcttgggatg cgtggctggc acttctttcg tttcgtcgaa tggccgtggt tacggcgaca
     8881 aatcccgcca gttgctgcgc ttatctttat gctctgtttc gccagcttcg ccaccgtgct
     8941 atcgctgggc ggcggtccgc aggcgaccac tatcgaactg gctatttatc aggcgctgag
     9001 ttacgactac gatcccaccc gcgcggcgat gctggcgctg atccagatgg tgtgttgcct
     9061 tgggctggtg ctgttgagtc agcgattgag taaggccatt gcgcccggca ctacgctgct
     9121 gcaaggctgg cgcgacccgg acgatcgtct gcatagccgc atttgcgaca cggtgttaat
     9181 tgtgctggcg ctgttgctgt tgctgccacc gttactggcg gtgctcgtcg atgggataaa
     9241 tcgccagttg ccggaagtgc tggcgcagcc ggtgctgtgg caggcgctgt ggacctcgtt
     9301 gcgtattgcg ctggcggcag gcgtattatg cgtagtgctg accatgatgc tgctatggag
     9361 cagtcgcgaa ctgcgggcgc ggcagaaaat gctggcgggt caggcgctgg agatgagcgg
     9421 catgttgatc ctcgcgatgc cggggattgt gctggctacc ggcttctttt tactgctcaa
     9481 caacactatc ggcctgccac aatctgctga cggcattgtg attttcacca atgcgttaat
     9541 ggcgatccct tatgcgctga aagtgctgga aaacccgatg cgcgatatca ccgcgcgtta
     9601 cagtatgttg tgtcagtcgc tgggcattga aggctggtca cgcttaaaag tggtggagct
     9661 gcgcgccctt aaacgcccac tggcgcaggc actggccttt gcttgtgtgc tatcgattgg
     9721 tgattttggc gtggtggcgt tgttcggtaa cgatgatttc cgcaccctgc cgttttatct
     9781 ctaccagcaa attggctcct atcgcagcca ggacggcgcg gtcaccgcgt taattctgct
     9841 gctactctgt tttctgctgt ttaccgtgat tgaaaaacta ccggggcgaa atgttaaaac
     9901 tgactgatat cacctggctt taccaccatt tgccgatgcg ttttagctta acggtggaac
     9961 gcagcgagca ggtggcgatc ctcgggccaa gcggcgcggg taaaagtacc ctgctgaatt
    10021 tgatcgccgg ttttctgacg ccagccagcg gttcgctgac tatcgacggc gttgatcaca
    10081 ccacgacgcc gccgtcacgc cgtccggtgt cgatgctgtt tcaggaaaac aacctgttca
    10141 gtcacctgac ggtcgcgcag aacatcgggc tggggcttaa tccaggattg aagctgaacg
    10201 cggcacagca ggagaaaatg cacgcgattg cccgtcagat ggggattgat aatttaatgg
    10261 cgcgattacc gggcgagctt tctggcggtc aacgacagcg cgtggcgtta gcgcgttgtc
    10321 tggtgcgcga acagccgatt ttattgctcg atgaaccgtt ttccgcgctc gatccggcgc
    10381 tacgtcagga gatgctgacg ctggtgagca cgagctgcca acagcaaaaa ataacgctat
    10441 tgatggtgtc gcacagtgtg gaagatgcgg cgcggatcgc cacgcgctcg gtggtggtcg
    10501 ccgacgggcg tatcgcctgg cagggtaaga ccgatgagtt gttgagcggt aaggcgagtg
    10561 cttcggcgct gttgggaatt aaaggttaat atgcactgtc ggatgcggcg tgaacgcctt
    10621 atccgaccta cgggtagtgc gttttgcagg cctgataaga cgcggcaagc gtcgcatcag
    10681 gcaacccgca caaggctatc ccctaaaccc caaccacttt acgcaaaata tcgatataca
    10741 ccggcatcaa cgggtggcga attaacacca ccagcgccac cacgccgatg gcagaaatca
    10801 acggcgtcag ccacaacaaa cgaccgcggg acaaataatg actcaaacgg tcagtcgctt
    10861 taccgctgcg ccataaccgc cagcacagcc agccaccaac ccacaaaaat accgccgtcg
    10921 ccagcagcaa ccatttaaac tcaccgctct gcatcccggc aggaatatcg atcgccgcgc
    10981 ccgccagaat ccctggcagg aagtaaaacg gcggccacag caggcagccg ataatattcg
    11041 gcgtgataaa ttttgccacc ggcagatcca gcattcccgc caccattggc accagcggac
    11101 gcgtcgggcc aacaaagcga ccgaccagaa tggtgaacat gctgtgttga tgcaacgcat
    11161 gttcagtttt atcgagtaat gctttgtttt tcttcagaaa tgaccagcga tgcaatggct
    11221 ttttaaaacg ccagcccagc cagaaggaga tccagtcgcc cagcaggcaa ccgacgatcc
    11281 ctgccagcca ggcgtgccag aaacttaact cgccactgcc aatcagcgcc cccagtcccg
    11341 ccatcagcac cgtaccgggt agaatcaaac cgaccagcgc cagcgactcc agaaaggcca
    11401 ccaacaccac cgccatcaat gaatacacgg tggattgggt aataaagtgt tccagcaatg
    11461 cttgcataat gtgcctgtca aatggacgaa gcagggattc tgcaaaccct atgctactcc
    11521 gtcaagccgt caattgtctg attcgttacc aattatgaca acttgacggc tacatcattc
    11581 actttttctt cacaaccggc acggaattca ctcgggctgg ccccggtgca ttttttaaat
    11641 actcgcgaga aatagagttg atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc
    11701 atccgggtgg tgctcaaaag cagcttcgcc tgactaatgc gttggtcctc gcgccagctt
    11761 aagacgctaa ttcctaactg ctggcggaac agatgcgaca gacgcgacgg cgacaggcaa
    11821 acatgctgtg cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac
    11881 tgacaagcct cgcgtacccg attatccatc ggcggatgaa gcgactcgtt aatcgcttcc
    11941 atgcgccgca gtaacaattg ctcaagcaga tttatcgcca gcaattccga atagcgccct
    12001 tccccttgcc cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct
    12061 tcatccgggc gaaagaaacc cgtattggca aatattgacg gccagttaag ccattcatgc
    12121 cagtaggcgc gcggacgaaa gtaaacccac tggtgatacc attcgcgagc ctccggatgg
    12181 cgaccgtagt gatgaatctc tcctggcggg aacagcaaaa tatcacccgg tcggcagaca
    12241 aattctcgtc cctgattttt caccaccccc tggccgcgta tggtgagatt gagaatataa
    12301 cccttcattc ccagtggtcg gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc
    12361 gttaaacccg ccaccagatg ggcgttaaac gagtatcccg gcagcagggg atcattttgc
    12421 gcttcagcca tacttttcat actcccgcca ttcagagaag aaaccaattg tccatattgc
    12481 atcagacatt gccgtcactg cgtcttttac tggctcttct cgctaatcca accggtaacc
    12541 ccgcttatta aaagcattct gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac
    12601 aaaagtgtct ataatcacgg cagaaaagtc cacattgatt atttgcacgg cgtcacactt
    12661 tgctatgcca tagcattttt atccataaga ttagcggatc ctacctgacg ctttttttcg
    12721 caactctcta ctgtttctcc atacccgttt ttttggatgg agtgaaacga tggcgattgc
    12781 aattggcctc gattttggca gtgattctgt gcgagctttg gcggtggact gcgccaccgg
    12841 tgaagagatc gccaccagcg tagagtggta tccccgttgg cagaaagggc aattttgtga
    12901 tgccccaaat aaccagttcc gtcatcatcc gcgtgactac attgagtcaa tggaagcggc
    12961 gctgaaaacc gtgcttgcag agcttagcgc cgagcagcgc gcagctgtgg tcgggattgg
    13021 cgttgacaca accggctcga cgcccgcacc gattgacgcg gacggtaacg tcctggcgct
    13081 gcgcccggag tttgccgaaa acccgaacgc gatgttcgta ttgtggaaag accacaccgc
    13141 ggttgaagaa gcggaagaga taacccgctt atgtcacacg ccgggcaacg ttgactactc
    13201 ccgctatatt ggcggtattt attccagcga atggttctgg gcaaaaatcc tgcatgtgac
    13261 tcgccaggac agcgccgtgg cgcaatctgc cgcatcgtgg attgagctgt gcgactgggt
    13321 gccagctctg ctttccggta ccacgcgccc gcaggaaatt cgtcgcggac gttgcagcgc
    13381 cgggcataaa tctctttggc acgaaagctg gggcggcctg ccgccagcca gtttctttga
    13441 cgaactggac ccgatcctca atcgccattt gccttccccg ctattcactg acacctggac
    13501 tgccgatatt ccggtgggca ccttatgccc ggaatgggcg cagcgtctcg gcctacctga
    13561 aagcgtggtg atttccggcg gcgcgtttga ctgccatatg ggcgcagttg gcgcaggcgc
    13621 acagcctaac gctctggtaa aagttatcgg tacttccacc tgcgacattc tgattgccaa
    13681 caaacagagc gttggcgagc gggcagtgaa aggtatttgc ggtcaggttg atggcagcgt
    13741 ggtgcctgga tttatcggtc tggaagcagg ccaatcggcg tttggtgata tctacgcctg
    13801 gttcggtcgc gtactcggct ggccgctgga acagcttgct gcccagcatc cggaactgaa
    13861 agagcaaatc aacgccagcc agaaacaact gcttccggcg ctgaccgaag catgggccaa
    13921 aaatccgtct ctggatcacc tgccggtggt gctcgactgg tttaacggcc gccgcacgcc
    13981 aaacgctaac caacgcctga aaggggtgat taccgatctg aacctcgcta ccgacgctcc
    14041 gctgctgttc ggcggattga ttgctgccac cgcctttggc gcacgcgcaa tcatggagtg
    14101 ctttaccgat caggggatcg ccgtcaataa cgtgatggcg ctgggcggca tcgcgcggaa
    14161 aaaccaggtc attatgcagg cctgctgcga cgtgctgaat cgcccgctgc aaattgttgc
    14221 ctctgaccag tgctgtgcgc tcggtgcggc gattttcgct gccgtcgccg cgaaagtgca
    14281 cgcagacatc ccctcagccc agcaaaaaat ggccagtgcg gtagagaaaa ccctgcaacc
    14341 gcgcagcgaa caggcacaac gctttgaaca gctttatcgc cgctatcagc aatgggcgat
    14401 gagcgccgaa caacactatc ttccaacttc cgccccggca caggctgccc aggccgttcc
    14461 gactctataa ggacacgaca atgacgattt ttgataatta tgaagtgtgg tttgtaattg
    14521 gcagccagca tctgtatggc ccggaaaccc tgcgtcaggt cacccaacat gccgagcacg
    14581 tcgttaatgc gctgaatacg gaagcgaaac tgccctgcaa actggtgctg aaaccgctgg
    14641 gcaccacgcc ggatgaaatc accgctattt gccgtgacgc gaattacgac gatcgttgcg
    14701 ctggcctggt agtgtggctg cacactttct ccccggccaa aatgtggatc aacggcctga
    14761 ctatgctcaa caaaccgttg ctgcaattcc acacccagtt taacgcggcg ctgccgtggg
    14821 atagcatcga tatggacttt atgaacctga accagactgc acatggcggt cgcgagttcg
    14881 gcttcattgg cgcgcgtatg cgtcagcaac atgccgtcgt taccggtcac tggcaggata
    14941 aacaagccca tgagcgtatc ggctcctgga tgcgtcaggc ggtctctaaa caggataccc
    15001 gtcatctgaa agtctgccgt tttggcgata acatgcgtga agtggcggtc accgatggtg
    15061 ataaagttgc cgcacagatc aagttcggtt tctccgtcaa tacctgggcg gttggcgatc
    15121 tggtgcaggt agtgaactcc atcagcgatg gcgatgttaa cgcgctggtc gatgagtacg
    15181 aaagctgcta caccatgacg cctgccacac aaatccacgg cgaaaaacgc cagaacgtgc
    15241 tggaagcggc gcgtattgag ctggggatga agcgtttcct ggaacaaggt ggcttccacg
    15301 cgttcaccac cacctttgaa gatttgcacg gtctgaaaca gcttcctggt ctggccgtac
    15361 agcgtctgat gcagcagggt tacggctttg cgggcgaagg cgactggaaa actgccgccc
    15421 tgcttcgcat catgaaggtg atgtcaaccg gtctgcaggg cggcacctcc tttatggagg
    15481 actacaccta tcacttcgag aaaggcaatg acctggtgct cggctcccac atgctggaag
    15541 tctgtccgtc gatcgctgtg gaagagaaac cgatcctcga cgttcagcac ctcggtattg
    15601 gcggtaaaga cgatcctgcc cgcctgatct tcaataccca aaccggtcca gcgattgtcg
    15661 ccagcctgat tgatctcggc gatcgttacc gtctgctggt taactgcatc gacacggtga
    15721 aaacaccgca ctccctgccg aaactgccgg tggcgaatgc gctgtggaaa gcgcaaccgg
    15781 atctgccaac tgcttccgaa gcgtggatcc ttgctggtgg cgcgcaccat accgttttca
    15841 gccatgcgct gaacctcaac gatatgcgcc agttcgccga gatgcacgac attgaaatca
    15901 cggtgattga taacgacacc cgcctgccag cgtttaaaga cgcgctgcgc tggaacgaag
    15961 tgtattacgg gtttcgtcgc taagtagccg catcaggtgt gtaacgcctg atgcgacgct
    16021 gacgcgtctt atcaggccta cacgctgtga ttttgtaggc cggataagca aagcgcatcc
    16081 ggcattcacc gcctgatgcg gcgctggcgc gtcttatcag gcctacgcgt tccggatttg
    16141 taggccggat aagcaaagcg catccggcat tcaccgcctg atgcgacgcc gacgcgtctt
    16201 atcaggccta cacgctgcga ttttgtaggc cggataagca aagcgcatcc ggcacgaagg
    16261 agtcaacatg ttagaagatc tcaaacgcca ggtattagaa gccaacctgg cgctgccaaa
    16321 acacaacctg gtcacgctca cctggggcaa tgtcagcgcc gttgatcgcg ggcgcggcgt
    16381 cctggtgatc aaaccttccg gcgtcgacta cagcatcatg accgctgacg atatggtcgt
    16441 ggtcagcatc gaaaccggtg aagtggttga aggaacgaaa aagccctcct ccgacacgcc
    16501 aactcaccgg ctgctctatc aggcattccc gtccattggc ggcattgtgc acacacactc
    16561 gcgccacgcc accatctggg cgcaggcggg ccagtcaatt ccggcaaccg gtaccaccca
    16621 cgccgactat ttctacggca ccattccctg cacccgcaaa atgaccgacg cagaaatcaa
    16681 cggtgaatat gagtgggaaa ccggtaacgt catcgtagaa accttcgaaa aacagggaat
    16741 cgacgcagca caaatgcccg gcgtcctggt ccattctcac ggcccatttg catggggcaa
    16801 aaatgccgaa gatgcggtgc ataacgccat cgtgctggaa gaggtcgctt atatggggat
    16861 attctgccgt cagttagcgc cgcagttacc ggatatgcag caaacgctgc tggataaaca
    16921 ctatctgcgt aagcatggtg cgaaggcata ctacgggcag taatgactgt ataaaaccac
    16981 agccaatcaa acgaaaccag gctataatca agcctggttt tttattggat tttcagcgtg
    17041 gcgcaggcag gttttatctt aacccgacac tggcgggaca ccccgcaagg gacagaagtc
    17101 tccttctgga tggcaacgga taacgggccg ttgcaggtca cgcttgcacc gcaagagtcc
    17161 gtggcgttta ttcctgccga tcaggttccc cgcgctcagc atattttgca gggtgaacaa
    17221 ggctttcgcc tgacgccgct ggcgttaaag gattttcacc gccagccggt gtatggcctt
    17281 tactgtcgcg cccatcgcca gctaatgaat tacgaaaagc gcctgcgtga aggtggcgtt
    17341 accgtctacg aggccgatgt ccgcccgccg gaacgctatc tgatggagcg gtttatcacc
    17401 tcgccagtgt gggtcgaggg tgatatgcac aatggcgcta tcgttaatgc ccgtctgaaa
    17461 ccgcatcccg actatcgtcc gccgctcaag tgggtttcta ttgatattga aaccacccgc
    17521 cacggtgagc tgtactgcat cggcctggaa ggctgcggtc aacgcatcgt ttatatgctg
    17581 gggccggaga atggcgacgc ctccgcgctc gatttcgaac tggaatacgt cgccagccgc
    17641 ccgcagctac tggaaaaact taacgcctgg tttgccaact acgatcctga tgtgatcatc
    17701 ggttggaacg tggtgcagtt cgatctgcga atgctgcaaa aacatgccga gcgttaccgt
    17761 attccgctgc gtctggggcg tgataacagc gagctggagt ggcgcgagca cggctttaaa
    17821 aacggcgtct tttttgctca ggccaaaggg cgactgatta tcgacggtat cgaggcgctg
    17881 aaatccgcgt tctggaattt ctcttcattc tcgctggaaa ccgtcgctca ggagttatta
    17941 ggcgaaggaa aatctatcga taacccgtgg gatcgaatgg acgaaattga ccgccgtttc
    18001 gccgaagata agcctgcgct ggcaacttat aacctgaaag attgcgagct ggtgacgcag
    18061 atcttccaca aaactgaaat catgccgttt ttgctcgaac gggcgacggt gaacggcctg
    18121 ccggtagatc gacacggcgg ttcggtggca gcgtttggtc atctctattt tccccgtatg
    18181 catcgcgctg gttatgtcgc gcctaatctc ggcgaagtgc cgccgcacgc cagccctggc
    18241 ggctacgtga tggattcgcg acctgggctt tatgattcgg tgctggtgct ggactacaaa
    18301 agcctgtacc cgtcgatcat ccgcaccttt ctgattgatc ccgtcgggct ggtggaaggc
    18361 atggcgcagc ctgatccaga gcacagcacc gaaggctttc tcgatgcctg gttctcgcga
    18421 gaaaaacatt gcctgccgga gattgtgact aacatctggc acgggcgcga tgaagccaaa
    18481 cgtcagggta ataagccgct ttcgcaggcg ctgaagatca ttatgaatgc cttttatggc
    18541 gtgcttggca ccaccgcctg ccgcttcttc gatccgcgcc tggcgtcgtc gatcactatg
    18601 cgtggtcatc agatcatgcg gcaaaccaaa gcgttgattg aagcgcaggg ctacgacgtg
    18661 atctacggcg ataccgactc aacgtttgtc tggttgaaag gcgcacattc ggaagaagaa
    18721 gcggcgaaaa ttggtcgtgc actggtgcag cacgttaacg cctggtgggc agaaacgctg
    18781 caaaaacaac ggctaaccag cgcactggaa ctggagtatg aaacccattt ctgccgtttt
    18841 ctgatgccaa ccattcgcgg agccgatacc ggcagtaaaa aacgttatgc cgggctgatt
    18901 caggagggcg acaagcagcg gatggtgttt aaagggctgg aaaccgtgcg caccgactgg
    18961 acgccgctgg cccagcagtt tcagcaggag ctgtacctgc gtatcttccg caacgagcca
    19021 tatcaggagt acgtccgcga aaccatcgac aaactgatgg cgggtgaact ggatgcgcgg
    19081 ctggtttacc gtaaacgcct tcgccgtccg ctgagcgaat atcagcgtaa tgttccgcct
    19141 catgtacgcg ccgctcgcct tgccgatgaa gaaaaccaaa agcgcggtcg ccccttgcaa
    19201 tatcagaacc gcggctccat taagtacgta tggaccacca acggcccgga gccgctggac
    19261 taccaacgtt caccgctgga ttacgaacac tatctgaccc gccagctaca acccgtggcg
    19321 gagggaatac tcccttttat tgaggataat tttgctacac ttatgaccgg gcaacttggg
    19381 ctattttgag caaaaaaaga gttcgccaga taccattttg atgcgtgacg aatgctttgc
    19441 catccagtac catagcgccc tttccattcc tggacctgaa taacaccact acctcataag
    19501 cacggtagcg ggtggttatt gcctgcaatt aaagatatag agccgaacac atatgccttt
    19561 tacacttggt caacgctgga tcagcgatac agaaagcgaa ttgggacttg gaaccgttgt
    19621 cgcggtggat gcgcgaactg tcactttact tttcccatct actggtgaaa accgtctgta
    19681 cgcacgcagt gattcccccg tgacccgcgt gatgttcaac cctggtgata ccattaccag
    19741 ccatgacggc tggcagatgc aagtcgaaga agtaaaagaa gaaaatggct tgctgaccta
    19801 tatcggtact cgccttgata ctgaagagtc cggcgtggcc ctgcgtgaag ttttccttga
    19861 tagcaaactg gtgttcagca aaccgcagga ccgtctgttt gccgggcaga ttgaccgtat
    19921 ggaccgcttt gcgctgcgtt atcgcgcgcg taagtattcc agcgaacagt tccgtatgcc
    19981 gtacagcggc ctgcgcggtc agcgtaccag cctgatcccg caccagctca acatcgctca
    20041 tgatgttggt cgccgccacg cgccgcgcgt cctgttggct gacgaagtgg gtttagggaa
    20101 aaccattgaa gccgggatga tcctgcatca gcaactgctc tctggcgctg ctgaacgtgt
    20161 gctgattatc gtcccggaaa ccttacagca tcagtggctg gtagaaatgc tgcgccgttt
    20221 caacctgcgc tttgctctgt ttgatgatga gcgttatgcc gaagctcagc acgatgctta
    20281 caacccgttc gataccgagc agctggtgat ttgctcgctg gattttgccc gtcgtagcaa
    20341 acagcgtctg gaacatctct gtgaagccga gtgggatcta ctggtggtcg atgaagcgca
    20401 tcacctggtg tggagcgaag atgcgccgag ccgtgaatat caggccattg aacaactggc
    20461 agagcacgtg ccgggcgttc tgctgctgac cgcaaccccg gaacagctgg ggatggaaag
    20521 ccacttcgcc cgtctgcgtc tgctggaccc gaaccgtttc cacgactttg cgcaattcgt
    20581 tgaagagcag aaaaattatc gtccggttgc ggacgccgtt gccatgctgc tggcaggtaa
    20641 caaactcagc aatgacgaac tgaacatgct cggtgagatg atcggcgagc aggatatcga
    20701 gccgctgttg caagcagcaa acagcgacag cgaagatgcc cagagcgccc gtcaggaact
    20761 ggtttcgatg ctgatggatc gccacggcac cagccgcgtg ctgttccgta acacccgtaa
    20821 cggtgtgaaa ggcttcccga aacgcgagct gcatactatt aagctgccac tgccgacgca
    20881 gtatcagacc gctattaaag tctccggcat tatgggcgca cgtaaaagtg cggaagatcg
    20941 cgctcgcgat atgctctacc cggagcgtat ttatcaggaa tttgaaggcg ataacgccac
    21001 ctggtggaac ttcgatccgc gcgttgaatg gctgatgggc tacctgacca gccatcgctc
    21061 tcagaaagtg ctggtgatct gcgccaaagc ggccactgcg ctgcaactgg agcaggtact
    21121 gcgcgaacgt gaaggtattc gcgccgcagt gttccacgaa ggcatgtcga ttatcgaacg
    21181 tgaccgcgct gccgcctggt ttgccgaaga agacaccggc gcacaggtac tgctgtgctc
    21241 ggaaatcggt tctgaaggac gtaacttcca gttcgccagc cacatggtga tgtttgacct
    21301 gccattcaac ccggatctgc tggagcagcg tattggtcgt ctggatcgta tcggtcaggc
    21361 gcacgatatt cagatccatg tgccttatct ggaaaaaacg gctcagtcgg tgctggtgcg
    21421 ctggtatcac gaaggtctgg atgcattcga gcacacctgc ccgaccggac gcactatcta
    21481 cgatagcgtg tacaacgatt tgattaacta tctggcctcg ccggatgaga ccgaaggctt
    21541 tgacgatctg atcaaaaact gccgcgagca acatgaagcg ctgaaagcac agctggaaca
    21601 aggtcgtgac cgcctgctgg aaatccattc caacggtggc gaaaaagccc aggcactggc
    21661 agaaagcatt gaagagcagg atgacgatac caacctgatc gccttcgcca tgaacctgtt
    21721 cgatattatc ggtatcaatc aggacgatcg cggcgacaac atgatcgtgc tgacgccgtc
    21781 cgatcatatg ctggtgccgg acttccctgg cctgtcggaa gatggcatca ccatcacctt
    21841 cgaccgtgaa gtggcgctgg cgcgtgaaga tgcacagttt attacctggg agcatccgct
    21901 gatccgcaac ggtctggatc tgatcctttc tggcgatacc ggtagcagca cgatttcact
    21961 gttgaaaaac aaagcgttgc cggtaggtac gctgttggtg gaactgattt atgtggttga
    22021 agcccaggct ccgaagcagt tgcagctcaa ccgcttcctg ccaccgacgc cggtacgtat
    22081 gctgctggat aaaaacggca acaacctggc ggcgcaggta gagtttgaaa cctttaaccg
    22141 ccagcttaac gcggttaacc gtcacaccgg cagcaagctg gttaacgcgg tgcagcagga
    22201 tgttcacgct atccttcagc tgggggaagc gcagatcgag aaatctgccc gtgcattgat
    22261 tgatgccgca cgtaacgaag ccgacgaaaa actgtctgcc gagctgtctc gtctggaagc
    22321 gctgcgtgca gttaacccga acattcgtga cgacgaactg accgccattg agagcaaccg
    22381 tcagcaggta atggaaagcc tggatcaggc aggctggcgt ctggatgccc tgcgtttgat
    22441 cgttgtgacg catcagtaac ggagccgaag atggggatgg aaaactacaa tccgccgcag
    22501 gaaccctggc tggttgtcct gtatcaggat gaccatatta tggtggtcaa caagccgagc
    22561 ggtttgttgt ctgtgccggg tcgtctggaa gagcacaaag acagcgtgat gacgcgcatt
    22621 cagcgtgatt atccgcaggc agaatcggtg catcgtctgg atatggctac cagcggcgtg
    22681 attgtagtgg cgctgaccaa agccgcggag cgggagttaa aacgccagtt ccgcgagcgc
    22741 gagcctaaaa aacagtatgt ggcccgcgtc tgggggcatc catcccctgc ggaaggtctg
    22801 gtggatctgc cgctgatttg cgactggcca aaccgcccga aacagaaagt ctgttacgaa
    22861 acaggtaaac ctgcgcagac ggagtatgaa gtggtggaat atgcggcgga taacacggca
    22921 agagtggtgt taaaaccgat taccgggcgt tcgcatcagc tgcgtgtgca tatgctggcg
    22981 ctgggtcatc cgattctcgg cgatcgtttt tatgcatcac cagaagcgag agcaatggca
    23041 ccacgtttgt tgctacatgc agagatgctg acgattaccc atccggcgta tggcaatagt
    23101 atgacgttta aagcaccagc ggatttttaa tagtcacgct ggcgaagttc ggtaggccgg
    23161 agaaggcgtt cacgccgcat ccggcaattt tgcgccgatt gcctgatgcg acgcgggccg
    23221 cgtcttatca ggcctacacg ttgaaactta tttaaaccct ttctgctgct ttatcagctc
    23281 ataggcctgc tgaatttcct gcgctttctg cttcgccatc tccatcatct ctggcggcaa
    23341 acctttcgcc accaacttat cgggatggtg ttcactcatc agcttacggt aggcacgttt
    23401 gatggtggtc gcatcatccg tcggcttcac gcccagcaca ttacaggcat cttccagcgt
    23461 cgggccacgc tgcgcttgct gccagttacc accgccagat tgctgctgat aaccgccacc
    23521 aaactgtgca ccgccctgca tcatgcgcaa aaactggtca aactgagcgc gggagatccc
    23581 taattcttca gcaatgacat acagcaccgc ccgttcattc gggtgcagtg aaccatcagc
    23641 aaacgccgcc tgaatctgga tctccagaaa catacgaatt aagtcaaaac gaccaaagca
    23701 gacactgcga aactggcgca tcttttcgcg cagcgggtaa ttgtctgatt ttcccacccg
    23761 gaacgcattt tgcgccgcag tacgggaagc gccatgaagg ttcattcggt ccatcaactg
    23821 gctggcgata tgaatatcag cttccgtgac gcgacctttg gatttggtta aatgccccat
    23881 cacttcaaaa gtggtggcaa aaaacagcgc ctgacgctca cgctggttgg cgaaccacgc
    23941 cattttacgg ctacgggctt tatcaaacat atggccaatt aacaggccca atactacgcc
    24001 ccaaaagccg acgcccatta ttaaggccac ggccacgcca atgatttttc cccaatactg
    24061 catatattcc ccaaatcgac acacggatat cagggctatc tcccacaata taaaggcgct
    24121 tttacccatt tctggctgcg gtctattgtg agacatcgcc tataatttgc attatcatac
    24181 ctgtcattca atgccgtgcc taacaccaca gacgctattc ggacaggatt aacactagcg
    24241 tagagatgac gagtacgtta gtctctgacc gtttgtcacg cgcaacgtta ccgatgatgg
    24301 aacaataaaa tcaacgtatg aaaaaacgta tccccactct cctggccacc atgattgcca
    24361 ccgcccttta tagtcaacag ggactggcag ccgacctcgc ctcacagtgc atgttgggcg
    24421 tgccaagcta tgaccgtcct ctggtacagg gcgataccaa tgacttaccc gtgactatca
    24481 atgctgacca cgcgaaaggg gactacccgg atgacgccgt gtttactggc agcgtggata
    24541 tcatgcaggg taacagccgt ctgcaggccg acgaagtgca gctccatcaa aaagaggcac
    24601 caggacaacc ggagccggtg cgtaccgttg atgcgctcgg taatgtccat tacgacgata
    24661 accaggtgat cctcaaaggg ccgaaaggct gggcgaatct gaacaccaaa gataccaacg
    24721 tctgggaggg tgattaccag atggtgggtc gccagggccg cggtaaagcg gacctgatga
    24781 aacaacgtgg cgaaaaccgc tataccattc tggataacgg tagctttacc tcctgtctgc
    24841 cgggttctga tacctggagc gtggtaggta gcgaaattat tcatgaccgc gaagaacaag
    24901 ttgcggagat ctggaacgcc cgctttaagg tgggtccggt accgatcttt tatagcccct
    24961 atttgcagtt gccggtgggt gacaaacgtc gctctggttt cttgatcccg aacgccaagt
    25021 acaccaccac caactacttt gagttctacc tgccatatta ctggaacatc gcgccaaata
    25081 tggatgccac catcacgccg cattatatgc atcgtcgtgg caatatcatg tgggagaacg
    25141 aattccgcta cctctcccag gcgggcgctg gcttgatgga actggactat ctgccttcag
    25201 ataaagtcta tgaagatgaa cacccgaacg atgacagttc acgtcgttgg ttgttctact
    25261 ggaaacactc cggggtcatg gatcaggtgt ggcgtttcaa cgtcgactac accaaagtca
    25321 gcgatcctag ctacttcaat gatttcgata acaaatacgg ttccagtacc gacggctacg
    25381 caacgcaaaa attcagcatt ggctatgcgg tgcagaactt caatgccacc gtttcaacca
    25441 agcagttcca ggtcttcagc gagcagaaca ccagtagcta ctcggcagag ccgcagttag
    25501 acgttaacta ctaccagaat gatgttggtc cgtttgatac gcgtatttac ggccaggcag
    25561 tgcactttgt taacaccaga gatgacatgc cagaggcgac tcgtgttcac ctggaaccga
    25621 ccatcaatct gccgctctct aataactggg gcagcatcaa tactgaagcg aagttgctgg
    25681 caacccatta tcagcaaacc aatcttgact ggtataactc cagaaacacg accaggctgg
    25741 cagagtccgc taaccgtgta atgccgcagt tcaaggtaga cggtagaatg gtcttcgagc
    25801 gtgatatgga aatgctggcg ccgggctata cccagacact ggaaccacgc gcgcagtatc
    25861 tgtatgtgcc atatcgcgat cagagcaaaa tctataacta cgactcttcg ctgctgcaat
    25921 ctgactactc cggcctgttc cgggaccgga cttacggcgg tcttgaccgt attgcctcca
    25981 ctaaccaggt gacgaccggt gtcacatctc gcatatatga tgatgctgcc gttgaacgtt
    26041 ttaatatttc cgttggtcaa atctactatt tcacggagtc tcgcactggc gatgacaaca
    26101 taacatggga gaatgacgac aaaacgggct cactggtatg ggcaggcgat acttactggc
    26161 gcatctctga tcgttgggga ttgcgtggcg ggattcagta cgatacacgt ctggataacg
    26221 tagcgaccag taactccagc attgaatacc gtcgggatga agaccgtctg gtacagctga
    26281 attaccgtta cgccagcccg gaatatattc aggcaacgct gcctaagtac tattccactg
    26341 ctgagcaata taagaatggt atttcgcagg taggagccgt cgccagctgg ccaattgccg
    26401 atcgttggtc cattgttggg gcctactact acgacaccaa tgcgaacaag caagccgact
    26461 ctatgttagg tgtgcaatac agctcctgct gctatgcgat tcgcgtcggt tacgagcgga
    26521 agctgaacgg ttgggataac gataaacaac atgcggtata tgacaacgca atcggcttta
    26581 acatcgaact tcgcggcctg agctccaact acggtctggg tacgcaagag atgctgcgtt
    26641 cgaacattct gccgtatcaa aacactttgt gatttgttga tttaccacgt aatccgcagt
    26701 gcggttaatt gaaatggaaa aagtatgaag aactggaaaa cgctgcttct cggtatcgcc
    26761 atgatcgcga ataccagttt cgctgccccc caggtagtcg ataaagtcgc agccgtcgtc
    26821 aataacggcg tcgtgctgga aagcgacgtt gatggattaa tgcagtcggt aaaactgaac
    26881 gctgctcagg caaggcaaca acttcctgat gacgcgacgc tgcgccacca aatcatggag
    26941 cgtttgatca tggatcaaat catcctgcag atggggcaga aaatgggagt gaaaatctcc
    27001 gatgagcagc tggatcaggc gattgctaac atcgcgaaac agaacaacat gacgctggat
    27061 cagatgcgca gccgtctggc ttacgatggt ctgaactaca acacctatcg taaccagatc
    27121 cgcaaagaga tgattatctc tgaagtgcgt aacaacgagg tgcgtcgtcg catcaccatc
    27181 ctgccgcagg aagtcgaatc cctggcgcag caagtgggta accaaaacga cgccagcact
    27241 gagctgaacc tgagccacat cctgatcccg ctgccggaaa acccgacctc tgatcaggtg
    27301 aacgaagcgg aaagccaggc acgcgccatt gtcgatcagg cgcgtaacgg cgctgatttc
    27361 ggtaagctgg cgattgctca ttctgccgac cagcaggcgc tgaacggcgg ccagatgggc
    27421 tggggccgta ttcaggagtt gccggggatc ttcgcccagg cattaagcac cgcgaagaaa
    27481 ggcgacattg ttggcccgat tcgttccggc gttggcttcc atattctgaa agttaacgac
    27541 ctgcgcggcg aaagcaaaaa tatctcggtg accgaagttc atgctcgcca tattctgctg
    27601 aaaccgtcgc cgatcatgac tgacgaacag gcccgcgtga aactggaaca gatcgctgcc
    27661 gatatcaaga gtggtaaaac gacttttgct gccgcagcga aagagttctc tcaggatcca
    27721 ggctctgcta accagggcgg tgatctcggt tgggctacac cagatatttt cgatccggcc
    27781 ttccgtgacg ccctgacccg cctgaacaaa ggtcaaatga gtgcaccggt tcactcttca
    27841 ttcggctggc atttaatcga actgctggat acccgtaatg tcgataaaac cgacgcggca
    27901 cagaaagatc gtgcgtaccg catgctgatg aaccgtaagt tctcggaaga agcagcaagc
    27961 tggatgcagg aacaacgtgc cagcgcctac gttaaaatcc tgagcaacta atggttaaaa
    28021 cccaacgtgt tgtgatcact cccggcgagc ccgccgggat tggcccggac ttagttgtcc
    28081 agcttgcaca gcgtgagtgg ccggtcgaac tggttgtttg tgccgatgcc actctcctta
    28141 ccgaccgggc agcgatgctc ggtttgccgc tcaccctccg tccttattcc cccaactccc
    28201 ctgcacaacc gcaaactgcg ggcacattaa cgctacttcc tgtcgcgcta cgtgaatctg
    28261 tcactgcggg gcagttagcg gttgaaaatg ggcattacgt agtggagacg ctggcgcgag
    28321 cgtgtgatgg ctgtctgaac ggtgaatttg ctgcgctgat cacaggcccc gtgcataaag
    28381 gcgtcattaa cgacgcaggc attccgttta ccggtcatac cgagtttttc gaagagcgtt
    28441 cgcaggcgaa aaaagtggtg atgatgctgg cgaccgaaga acttcgcgtg gcgctggcaa
    28501 cgacgcattt accgctgcgc gatatcgcag atgctatcac ccctgcgctt ttgcacgaag
    28561 tgattgctat tttgcatcac gatttgcgga ccaaatttgg tattgccgaa ccgcgcattc
    28621 tggtctgcgg gctgaatccg cacgcgggcg aaggcggtca tatgggtacg gaagagatag
    28681 acaccattat tccggtgctc gacaagctgc gggcgcaggg gatgaaactc aacgggccgc
    28741 tgcctgccga taccctgttt cagccgaaat atcttgataa cgccgacgcg gtgctggcga
    28801 tgtaccacga tcagggtctt cccgtgctaa aataccaggg cttcgggcgc ggtgtgaaca
    28861 ttacgctggg cctgcccttt attcgcacat cagtggacca cggtaccgcg cttgaactgg
    28921 cgggacgtgg caaagccgat gtcggcagtt ttattacggc gcttaatctc gccatcaaaa
    28981 tgattgttaa cacccaatga ataatcgagt ccaccagggc cacttagccc gtaaacgctt
    29041 cgggcaaaac tttctcaacg atcagttcgt gatcgacagc attgtttctg ccattaaccc
    29101 gcagaagggc caggcgatgg tcgaaatcgg ccccggtctg gcggcattga ccgaaccggt
    29161 cggcgaacgt ctggataagc tcacggtcat cgaacttgac cgcgatctgg cggcacgtct
    29221 gcaaacgcat ccattcttag gcccgaaact gactatttat cagcaggatg cgatgacctt
    29281 taactttggt gaactggccg agaaaatggg tcagccgctg cgtgttttcg gcaacctgcc
    29341 ttataacatc tccacgccgt tgatgttcca tctgtttagc tatactgatg ccattgccga
    29401 catgcacttt atgttgcaaa aagaggtggt gaatcgtctg gttgcaggac cgaacagcaa
    29461 ggcgtatggt cgattaagcg tcatggcgca atactattgc aacgtgatcc cggtgctgga
    29521 agtaccgccg tcagccttta caccaccacc caaagtggat tccgccgtcg tgcgcctggt
    29581 tcctcatgca acgatgcctc acccggttaa agatgttcgc gtgttgagcc gcatcaccac
    29641 cgaagccttt aaccagcgtc gtaaaaccat tcgtaacagc ctcggcaacc tgtttagcgt
    29701 cgaggtgtta acgggaatgg ggatcgaccc ggcgatgcga gcggaaaata tctctgtcgc
    29761 gcaatattgc cagatggcga actatctggc ggagaacgcg cctttgcagg agagttaacg
    29821 atgatcaatt cgccccgagt gtgtattcag gttcaaagcg tctacattga ggcacaatct
    29881 tcacctgata atgaacgtta cgtttttgct tataccgtaa ccatacgcaa tctggggcga
    29941 gcgccagtgc agttgttggg gcgttactgg ctgatcacca atggcaatgg ccgtgaaacc
    30001 gaagtccagg gcgaaggagt ggttggcgtc cagccactta tcgcacctgg cgaagagtac
    30061 cagtacacca gcggtgcaat cattgaaacc ccgctgggca ccatgcaggg tcactacgaa
    30121 atgatcgatg aaaatggcgt ccctttcagc atcgacattc ccgtattccg actcgccgtt
    30181 cccacactca ttcattaaaa gaatatggcg acatacctta ttggcgacgt tcatggttgt
    30241 tacgatgaac tgatcgcatt gctgcataaa gtagaattta cccctgggaa agataccctc
    30301 tggctgacgg gcgatctggt cgcgcgcggc ccgggttcgc tggatgttct gcgctatgtg
    30361 aaatccttag gcgacagcgt acgtctggtg ctgggcaatc acgatctgca tctgctggcg
    30421 gtatttgccg ggatcagccg caataaaccg aaagatcgcc tgacaccgct gctggaagcg
    30481 ccggatgccg acgagctgct taactggctg cgtcgccagc ctctgctgca aatcgacgaa
    30541 gagaaaaagc tggtgatggc ccacgccggg atcacgccac agtgggatct acagaccgcc
    30601 aaagagtgcg cgcgcgatgt agaagcggtg ctgtcgagcg actcctaccc cttcttcctt
    30661 gatgccatgt acggcgatat gccaaataac tggtcaccgg aattgcgggg gctgggaaga
    30721 ctgcggttta tcaccaacgc ctttacccgt atgcgttttt gcttcccgaa cggtcaactg
    30781 gatatgtaca gcaaagaatc gccggaagag gcccctgccc cactgaaacc gtggtttgcg
    30841 attcctggcc ctgtcgctga agagtacagc atcgcctttg gtcactgggc atcgctggaa
    30901 ggcaaaggta cgccggaagg tatttacgcg ctggatactg gctgctgctg gggtggtaca
    30961 ttaacctgcc tgcgctggga agataaacag tattttgtcc agccgtcgaa ccggcataaa
    31021 gatatgggtg agggagaggc ggccgcgtct taaatcccgt cgagcataaa atctaatgct
    31081 cctttaatca acgttcggtg cgcgctggca tcgcaaaatt tcgttcccag cgcgttaact
    31141 ggaacagttg cagtctcatg cgtcatgagc cgtctaccag taaaaggatt gtgttaagcc
    31201 gttctggagg gcgaatacgg ataaatcgtt caataggtgt taatggaata acaatacggc
    31261 gattaatctc accaataatg tcgctggtaa catcgataac aaagggaaac gcggcgttcc
    31321 tgccgcgact gcggtatacc gtaaattgca tcgctagaag ctccggtact catcagagaa
    31381 acatccggtt tcatcagcca attgatttaa cgcagcgata gcttctgcgt tctcttcacg
    31441 ccaacgtatt gctgcatgtt ttttaagttc agcattgagc gcaaccgtaa gggtggcgct
    31501 catatttaag cctgcatcac gagcgcgatt gactaactca cggtcgacgg tgacggtcac
    31561 actttgtgtg gtacgttttg cagtcatgat atcgtgctcc tgtacaattt atacgtctag
    31621 taaacacgga tatctgcacg tatcagtgat gccggaattc taatatacaa aattaccgcc
    31681 gctccagaat ctcaaagcaa tagctgtgag agttctgcgc atcggcatcg tggaattcac
    31741 tgaataccga ttcccagtca tccggctcgt aatccgggaa atgggtgtcg ccttccactt
    31801 ccgcgtcgat atgcgtcaga tacagttttt gcgctttcgg caggaactgc tcataaacgc
    31861 gaccgccgcc aatcaccatg atttctggta cgtcaccaca cgccgcaatg gcttcatcca
    31921 ccgacttcac ccacgttacg cgatcgtccg tacccggttg actgctgagg ataatatttt
    31981 tgcgtcctgg caacggacga ccgatggatt cccaggtatg gcggcccata atcacgggtt
    32041 tatttaaagt gttgcgttta aaccaggcga gatcggcagg caggttccac ggcatggcgt
    32101 tttccatgcc gataacacga tctaccgcta acgccgcaat cagactgatc attgagattt
    32161 cccgataaaa aaaattgtcg ccactatacg taaagcgtaa accgtcgtcg actggtgcga
    32221 ggatgatgtt gaggaaaatt ttatattctg ctggcgagtc cacgctctcc cactggactc
    32281 gccgcattac aatgaaataa aaacaattag ttagctgtaa agtgtgattt acgtcactct
    32341 ttattaggat gagggtttcg tttccggttc atccgccatg ttgccggtat gtttaccttc
    32401 ttcggttccc tgccagccat gtcgttgaat taatgacaga tgttcgcggt cctcggtaat
    32461 gatctcactt aacatcgcgc tggtgcgttt atagaccgcc gcgcgggctt tggtgtcgtt
    32521 ctcaaccatt gccatctctt ccaccatctg aatattaaag cggcggaaca catcggcgcg
    32581 ttctcgcgct tcatacggcc ccagacctaa actttccagt gccagacgcc cggttttcag
    32641 cgcaccttca aaggtttcac gctccggctt ttcaacgcct gcctgacgca aacgaatgta
    32701 gtggtcgaca tcgcgggcgc gggcaataat ctgcaaatgc gggaaatgtt ctttcaccat
    32761 ctccgtcagt tgcaggttgg tttgcggatc gtcgatagcg ttaatcagca cttccgcttt
    32821 cgccgctccg gcagattcca gtaaatccat ccgcgtggca tcgccataaa acactttcat
    32881 accaaattta cgcaaggttt cgatatggtc cggatcgtga tcgagcacca ccattttcac
    32941 cccgctggag agcagtaaac gtccggtgat ctgcccaaaa cgaccgaatc cggcgatgat
    33001 tacccgcggc tgttcttcgt cgatctcatc ggcttcacgc gcctcttcac tagaagattg
    33061 ctcaaggcga ttgaggatca ccagcagaat cggcgttgcc gccatcgaca gcgccaccgc
    33121 cagggtcagc gatttcgccc actccggctc cagcacattc gccatctgcg ccgcaccaaa
    33181 tactacaaag gcaaactcac taccctgccc taatagcacc gcaaaccagc gacgctgttt
    33241 atttggcacc tgcaacggcc gggcaatcag ccacagcatg gcgattttga tgatgaggaa
    33301 accgagcagc aaaatgacaa tgcgcaacgg attttcaagc agcgtgccaa agtctatcga
    33361 catgccaacg ccgatgaaaa acagccccag cagcaaacct ttaaatggtt cgatatcgct
    33421 ttccagcgca tgacggtatt ccgagcttgc cagcagtacg cccgccagaa acgcgcccat
    33481 cgccatcgac aagccgacct cttccagcag caaaccaaag ccaaacacga ggaataacgc
    33541 cacggcacta aacacttccc gcaagccaga gcgggcaaca aaacgcagtg ccggacgcgt
    33601 gacatagcgc cccagcaata ccaccagcac cagcgcaccc gccactttta acgccgagag
    33661 agcaaatgcg cccatcgtcg tcgaggcact gctcgccgcc agtagcggga tcattgccac
    33721 cagcgggatc gccgcgatat cctggaacag cagcaccgca aaggcactgc gacccatttg
    33781 cgtcaccatc agattgcgtt cattcatcgc ctgcatggca atcgccgtag aggagagcgc
    33841 cagcgtcatg ccgatcaatt ccgcgacctg ccagcgcagc ccaagcaaca tacagaacag
    33901 ccccagcagg ccgccgcaaa tcaccatctg caatgcgccg ccgccgaaca ccgccgcacg
    33961 cagcttccac agcctttgtg gatcgagttc gaggccgata ataaacagca tcagcaccac
    34021 gccaatctcg gcaaagtgca gaatagattc ggcatcggtc accagtcgca gcccccacgg
    34081 gccaataatg cagccggcga tcaggtagcc aagtaccgat cccagaccga gacgtaccgc
    34141 aatgggaaca atcagcgccg ccgaaccgag ataaatcagc gcctgaataa gcgtatggct
    34201 atccatgatg tgcctcctgc cattccagca gacgttgctt atagtgccgc gcctgccctt
    34261 cgagggtttc gtcgtcacaa ataaaggtgc agtgcatggc aaacggtggc agccagttca
    34321 gcccgcagta gattgccgtc gcctgtagcg gctgtgacag cacatcaaag cccggatgcg
    34381 caccaatctc aaaatggctt tccccaccgc cggtcgtcac cgcccacagt aaatgtttgc
    34441 catgcagcgc cgtgccgccg tgaccgtaag cccagccgtg cgagaagact ttatcgatcc
    34501 aaagtttaag aagcggagga atgctgtacc actgcatcgg gtgctgccag acgatcagat
    34561 cggcgcgaga cagcgcctcc tgctcggcgg caatatcgat attgaagtca ggatagagtt
    34621 gataaagaga gcgaatttcg acgccttcca gcgtccttgc ctgttcaagc atccgtttat
    34681 tcgcatggga atgatgcgga tacggatgcg cataaattat aagaatcata ggtagcccgc
    34741 gtgattacgc ctttgttttc ccacaaagtg tagtcatata ttcacgcggc ttatagtcaa
    34801 taatattgat tcagaaaaga gagaaatctg aacaaattac cccattctct gcccgcggat
    34861 cgtcatattg ctggtctgcg ccagtgacat ccctcgagtc tccggggcaa acgctacgga
    34921 aatcaacaag ccaaacagcg agatacccgc ccccattagc atcgtgttac tgataccgta
    34981 attattgata aagatcggca gtgcccaggt cgaaacaata gtgccaatac ggcttaagga
    35041 cataatcacg cccacggcag aggcgcggat atccgtcggg aagagttcat taggatagag
    35101 ccactgcaaa ttacccggcc cgccagagaa aaaggcatac accgcaaatg ccatcactac
    35161 cagccagatc cccatatccg ggatcagccc caaaaccgcc agcgccagcg tcatcatggc
    35221 aaagctgcca atcaacaatg gacgccgtcc ggcagtgttt agccacagca tcggtggaat
    35281 acagccgagc ataaagaaca ggctaatcac cacgttcccc agtgccgcgt ttttgccaac
    35341 acccaatccc aacaaaccaa cgatttgcgg gccaaaggtg tagatggcga acatcgggat
    35401 cacctggcag gtccagatgg cggcaacaaa cagaacaaaa ggaaaatggc ggcgattaaa
    35461 cagatcgcga aaacgggttt gctgcggctg ctcttcatcg aaagccaccg gttcgccaaa
    35521 cagtttgatc atcatctctt cgcactcttt tactcgccct ttgcgtaaca accagcgggg
    35581 agattcaggc agttcgaatc gaccaatcaa aatcaacaaa caggggatcg ctgcgctacc
    35641 cagcatccag cgccaaccac cttccacatc ataaagccag taaccgacca gatcggcgca
    35701 ggtcgcgccg acataccaca tcgcggcaat aaagctgatg gaaaacgccc gctgacgggt
    35761 actggagaac tcagtgatca tcgaagtggc gatgggataa tctgcaccga tgacaatgcc
    35821 gatgagtacc cgcatcacta acagttcgac gggggatgag acaaacatcg tcgccaccga
    35881 tatcacgccg atggcgatga tatcgatgag gaacattttg cgccgtccga ctttatcgga
    35941 gatatagcca aacagcgatg tgccaacgaa cagcccggcg agcgttcccg cgcccagcaa
    36001 gccaatccag tcagcgtcca gtttcagcgc cggagtcagt tgctccagcg ccacgccaat
    36061 cattaccagt acataaccat ccagaaacgg accaccgctt ccccacagca aaatgcggcg
    36121 gtgaatagaa gagaatttaa gatcgtcaaa gtttctggac ggttgcatgg gttattcctg
    36181 tttttttgca atgagagtca gtacggcgtt acgtatcaaa ccaacatcag ccgtaacgga
    36241 actccacacc aaaggtgccg cgcgggtatt cccactgttc cagcgccgac cccagcccca
    36301 gaatgcgaca ggtgccgcac tccagacatc cggcgtaatc gaagcgcaca ctgccgtcat
    36361 cctgcttctt gtacagacct gcggggcacg ctttcaccag cagctccagg gcctgtttat
    36421 ctgcatcggc cttcacaacg atgtgcggat gctcttcatc gacattgaat ttattgacgc
    36481 ccagtttgac gtccacattg acgggagaag tcataaaacg gtcactcctt tcatgccatc
    36541 cttgatcaga ttgattaagc ccactttctt gccgtggcgg aggattttct tgcgcatcag
    36601 ttccggcgca ctgccgtcaa tggtgaacag gtcacgcgcc acgcccaccg ccagttccgg
    36661 gtagccgcta aacatgcgtg ggttatcaag gaacgccggt agtttctggt acatgcgcat
    36721 atcgcgcagc gggccactct caagatgctg acgatattcc gcgagttttt gcttactgaa
    36781 atcgtcgctt ttcatcgctg aaagcacagt ttttgctgct gcttccccgg cggcaatcgc
    36841 cagatccata ccgcgaatgg taaaaccgag gttcatacac attccggcag catcaccggc
    36901 aatcaatacg ccgtcaccaa ccaactccgg cagcatgttg atacctgctt ccggcactac
    36961 gtgagcggaa tattccacca gcttgccgcc cgcgatcagc ggtgcaacgg ccggatgttg
    37021 tttgaaatct tccagcattt gcggcaccga tttttttgcg tcatgcagat gatgcaaacc
    37081 acaaaccagc cccagcgaca gggtattttc attggtataa aggaagccac cgcccatcag
    37141 gccatcggtg ggtgatcccg caaacaggca agccgcgccc tgattaccct gcaactgaaa
    37201 acggtcttcg ataaccgact tcggtaactc gatcagttcc tttacgccaa ccgccacatc
    37261 cgtcggttta acgcgttttg ccatccccag cttttcggca aggatggaat tcaccccatc
    37321 agcaaggatc accgttttcg cttcaatcac atcgccatcg gcttctacac cgacgacttt
    37381 gccatcgcgc tgtacgaggt tatcgacgcg gatcccggta attaactgcg cgcccgcttc
    37441 ttcagcctgc tccatcagcc aggcatcaaa tttactgcgc aaaacggagt aagaacgctg
    37501 ggatggcgag gtttcgtcgc cattgcagta gtccatagtc atcgctgact tttccgtcat
    37561 aaacgcgagt ttttcatggg tgatcaggcg ttctacgggg gcggagtcag cgaaaccagg
    37621 aataatgtgt tccagactgt gggcatagag acgcccgccg gtgacgttct tggcacctgc
    37681 ggaattgcct cgctcgataa ctaacacttg cgcaccttcg cgggcgagca ctagtgcggc
    37741 aaccgaaccg gcaagccctg caccgacgat gatggcgtca aagatatctt cggacataag
    37801 agctccctgt cagcggcagg gccaaaatgc agccctgcca gagtggatca acgcgctaaa
    37861 gctgcggtca gtgccggaag gatcttcacg gcgtcgccaa caatgccgta atccgcgtac
    37921 tggaagatcg gcgcattttt atctttgttg atggcgaaaa tggtttgcga cgcattagcg
    37981 ccaaccatgt gctggatctg cccggagatc cccaccgcca ggtacagttc aggtttcagc
    38041 atcaggttgg agataccgac atagcgttcg tgctccatcc atttttcgtt ttccgccacc
    38101 ggacgagaac aggccaactc cgcacctatc gccttgcaaa gctgttctgc cagcgcaatg
    38161 ttctctttgc tgccaatacc gcgaccaacg ctgaccacca ggcgggcttt gtcgagatcg
    38221 acgctgttgc tctgacgcgc ctgggttgcc gtgcgggtaa tcgccacagc cggagcctgc
    38281 cactccacgg tgtgcgtttc gccagtgcgt gacgcgtctg gctgagccac atcgaacgtg
    38341 ccgctgctga tggtcagaac cgcatacggc gtggcaatgc gttcttcgcc aatcgccaga
    38401 ccgccgtaaa ccatgtgttt cactgtcgct ttaccgtcct gcacgctaac agcgctggca
    38461 tcgttagaca ccgccgcgtt aaggcggtaa cccagtttcg ccgccagtaa tttgccgcga
    38521 cgggtgtttg gcaacagcac cagaccgtct gcgccgtgct ggcgaatagt gtcagccatg
    38581 acaccggcgt aatcttcgat catccgctcg tccggtttgc cgcttaattt ccagacatga
    38641 ttagcaccga gctggattgc ctgtgcgccg tcggcatcat tgaggacgaa cgtgttgatt
    38701 tgattagcta aagcctgcgc accgttcatc agttccggca gacgagaagg ggtatcgctg
    38761 aatacccaga cttgagaaaa cgtgttcata gcatcccctg taattaaatg actttgcgaa
    38821 gattttcagc aaatgcggcg atctgttctt cgccgtcgcc ttcaatcacg atgcgctgac
    38881 gctcgcgctg tttcggcgcg gcaacctgtt gttctgacca ggctgcctct gcgttaaaac
    38941 cgatatccgc cgccgaccat acctggactg gctttttcgc cgcgccgaga atggctttca
    39001 tcgaaggaat ttgtggggag ttgatatcag tggaaacagc aacaaccgca ggcagcggaa
    39061 tgcttaaggt ttctgtttca tcttccagtt cgcgctcaac ggtgagggta tctgcagtca
    39121 gggagataat tttgctgacg ccgttaactg ccggaatatt aaggatttcg cctaccagca
    39181 aaccaacctg ctgggcataa aggtcggaag aaccatcgcc acagaggatc aaatcaaagc
    39241 ctgctttctg agcggctgca gccagtacgc tcgccgtttg ttgcggcagt gcctgctcga
    39301 actggtcatc aatcaccaca atcagttcat ccggaccgcg cgatagcaca tctttacgcc
    39361 ctttggcgtt ggtcagggct ttaccgccca cacttaaggc cgtcacctgc gcctctgctg
    39421 cctgttgctt tagctggcaa gccgcttcaa tagcgttgag atcgtattgg cttattttgg
    39481 catcggcttt gctgaagtct aacgaaccat cagcattatt gaccgcaata tcctgttcat
    39541 caggcacgca cttatagcaa gtaataatct tcattgcatc tccagaaatc atgaaggaaa
    39601 cgtcgttcat atcggtggtg ttaattacag atatcacgcc ctttaatttg gaaataaaac
    39661 tttaatcacc aatattgaaa atgtcacacg cagataaatc caactttcaa tattgttaag
    39721 ctcctcacca atattgaaaa catggcgtag aaaaaagaaa ttttcaatat tgttttatgg
    39781 atcaccaata ttgaaagtca gatgctattt ttttactcac aacagaacat aacaaactga
    39841 ttaataatca aaattaacga aaaaatgccc tctattcacc cgaaacaaaa atgtgataac
    39901 aatcacagaa tgcagcttat tgaataccca ttatgagtta gccat
//
LOCUS       DAPHCV010000033        39930 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.033, whole genome shotgun sequence.
ACCESSION   DAPHCV010000033 DAPHCV010000000
VERSION     DAPHCV010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.033"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(57..1916)
                     /gene="gss"
                     /locus_tag="RH033_001753"
     CDS             complement(57..1916)
                     /gene="gss"
                     /locus_tag="RH033_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HDV2990949.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFSVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE"
     gene            2121..2987
                     /gene="yghU"
                     /locus_tag="RH033_001754"
     CDS             2121..2987
                     /gene="yghU"
                     /locus_tag="RH033_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HDV2990950.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQHWAKEVGERPAVKRGRIVNRINGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3110..3358)
                     /gene="hybG"
                     /locus_tag="RH033_001755"
     CDS             complement(3110..3358)
                     /gene="hybG"
                     /locus_tag="RH033_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HDV2990951.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3371..3712)
                     /gene="hypA"
                     /locus_tag="RH033_001756"
     CDS             complement(3371..3712)
                     /gene="hypA"
                     /locus_tag="RH033_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HDV2990952.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPICHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3705..4193)
                     /gene="hybE"
                     /locus_tag="RH033_001757"
     CDS             complement(3705..4193)
                     /gene="hybE"
                     /locus_tag="RH033_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HDV2990953.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMLLSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4186..4680)
                     /locus_tag="RH033_001758"
     CDS             complement(4186..4680)
                     /locus_tag="RH033_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HDV2990954.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4680..6383)
                     /gene="hybC"
                     /locus_tag="RH033_001759"
     CDS             complement(4680..6383)
                     /gene="hybC"
                     /locus_tag="RH033_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HDV2990955.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6380..7558)
                     /gene="hybB"
                     /locus_tag="RH033_001760"
     CDS             complement(6380..7558)
                     /gene="hybB"
                     /locus_tag="RH033_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HDV2990956.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSAMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7548..8534)
                     /gene="hybA"
                     /locus_tag="RH033_001761"
     CDS             complement(7548..8534)
                     /gene="hybA"
                     /locus_tag="RH033_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HDV2990957.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSEHVQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8537..9655)
                     /gene="hybO"
                     /locus_tag="RH033_001762"
     CDS             complement(8537..9655)
                     /gene="hybO"
                     /locus_tag="RH033_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HDV2990958.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9844..10131)
                     /locus_tag="RH033_001763"
     CDS             complement(9844..10131)
                     /locus_tag="RH033_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HDV2990959.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKYCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10250..11137)
                     /locus_tag="RH033_001764"
     CDS             complement(10250..11137)
                     /locus_tag="RH033_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDV2990960.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVTRRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQSYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHYAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11294..12334
                     /gene="gpr"
                     /locus_tag="RH033_001765"
     CDS             11294..12334
                     /gene="gpr"
                     /locus_tag="RH033_001765"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HDV2990961.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNAIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLHEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12374..12868)
                     /locus_tag="RH033_001766"
     CDS             complement(12374..12868)
                     /locus_tag="RH033_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HDV2990962.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13059..13943
                     /gene="yghA"
                     /locus_tag="RH033_001767"
     CDS             13059..13943
                     /gene="yghA"
                     /locus_tag="RH033_001767"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HDV2990963.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEQDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAITDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(13982..14407)
                     /gene="exbD"
                     /locus_tag="RH033_001768"
     CDS             complement(13982..14407)
                     /gene="exbD"
                     /locus_tag="RH033_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HDV2990964.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14414..15148)
                     /gene="exbB"
                     /locus_tag="RH033_001769"
     CDS             complement(14414..15148)
                     /gene="exbB"
                     /locus_tag="RH033_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDV2990965.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15400..16587
                     /gene="metC"
                     /locus_tag="RH033_001770"
     CDS             15400..16587
                     /gene="metC"
                     /locus_tag="RH033_001770"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HDV2990966.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHVQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRNVVPDAIIMVDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16727..17386
                     /locus_tag="RH033_001771"
     CDS             16727..17386
                     /locus_tag="RH033_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV2990967.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17426..18325)
                     /gene="yqhC"
                     /locus_tag="RH033_001772"
     CDS             complement(17426..18325)
                     /gene="yqhC"
                     /locus_tag="RH033_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HDV2990968.1"
                     /translation="MKREEICRLLADKVNKLKNKENCLSGLLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     FDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18519..19682
                     /gene="yqhD"
                     /locus_tag="RH033_001773"
     CDS             18519..19682
                     /gene="yqhD"
                     /locus_tag="RH033_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDV2990969.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19787..20614
                     /gene="dkgA"
                     /locus_tag="RH033_001774"
     CDS             19787..20614
                     /gene="dkgA"
                     /locus_tag="RH033_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HDV2990970.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIVENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            20814..21740
                     /locus_tag="RH033_001775"
     CDS             20814..21740
                     /locus_tag="RH033_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HDV2990971.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQPD
                     ADHADAVVRFRIFKDDKEKTMQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     AIASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21791..22048
                     /gene="yqhH"
                     /locus_tag="RH033_001776"
     CDS             21791..22048
                     /gene="yqhH"
                     /locus_tag="RH033_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HDV2990972.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSDANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22090..24309)
                     /locus_tag="RH033_001777"
     CDS             complement(22090..24309)
                     /locus_tag="RH033_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HDV2990973.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRLQNAGAKKRPKAAVGR"
     gene            complement(24420..25832)
                     /gene="ftsP"
                     /locus_tag="RH033_001778"
     CDS             complement(24420..25832)
                     /gene="ftsP"
                     /locus_tag="RH033_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HDV2990974.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(25907..26644)
                     /gene="plsC"
                     /locus_tag="RH033_001779"
                     /gene_synonym="parF"
     CDS             complement(25907..26644)
                     /gene="plsC"
                     /locus_tag="RH033_001779"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HDV2990975.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(26878..29136)
                     /gene="parC"
                     /locus_tag="RH033_001780"
     CDS             complement(26878..29136)
                     /gene="parC"
                     /locus_tag="RH033_001780"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDV2990976.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRIRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDERRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29274..30881)
                     /locus_tag="RH033_001781"
     CDS             complement(29274..30881)
                     /locus_tag="RH033_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV2990977.1"
                     /translation="MYTRNLLWLVSLVSVAPLYAADVPANTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGIKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRTGDFMLSRQSWDATYNDASSFLNTLKS
                     DIEENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKTH"
     gene            complement(30990..31472)
                     /locus_tag="RH033_001782"
     CDS             complement(30990..31472)
                     /locus_tag="RH033_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDV2990978.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTERRLETDIYVPLA
                     "
     gene            complement(31525..31917)
                     /gene="ygiW"
                     /locus_tag="RH033_001783"
     CDS             complement(31525..31917)
                     /gene="ygiW"
                     /locus_tag="RH033_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HDV2990979.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            32069..32728
                     /gene="qseB"
                     /locus_tag="RH033_001784"
     CDS             32069..32728
                     /gene="qseB"
                     /locus_tag="RH033_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HDV2990980.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSIDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            32725..34074
                     /gene="qseC"
                     /locus_tag="RH033_001785"
     CDS             32725..34074
                     /gene="qseC"
                     /locus_tag="RH033_001785"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HDV2990981.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQANID
                     VRLTLNANGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADSFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFETKVSW"
     gene            34184..34765
                     /gene="mdaB"
                     /locus_tag="RH033_001786"
     CDS             34184..34765
                     /gene="mdaB"
                     /locus_tag="RH033_001786"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HDV2990982.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVFWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            34796..35110
                     /locus_tag="RH033_001787"
     CDS             34796..35110
                     /locus_tag="RH033_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDV2990983.1"
                     /translation="MITVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GL"
     gene            complement(35155..36042)
                     /locus_tag="RH033_001788"
     CDS             complement(35155..36042)
                     /locus_tag="RH033_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDV2990984.1"
                     /translation="MIQKDKVRVDIFGERFRTRASQLTPGLRVVASYINEHREVVLEQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTMLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(36039..36986)
                     /locus_tag="RH033_001789"
     CDS             complement(36039..36986)
                     /locus_tag="RH033_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HDV2990985.1"
                     /translation="MRLFFSLLILLSFFARATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIEQLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDADFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(36997..38034)
                     /locus_tag="RH033_001790"
     CDS             complement(36997..38034)
                     /locus_tag="RH033_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDV2990986.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLLLLGGLFLFAVGILIFGLMHGS
                     FSVPASEVGRALFSPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCARDISRPR
                     FVLIGIGVSWFFAAGIDVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(38043..39026)
                     /locus_tag="RH033_001791"
     CDS             complement(38043..39026)
                     /locus_tag="RH033_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDV2990987.1"
                     /translation="MMRTGLVTVIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFDP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGVTPMKVTLCGVALSAFVSSI
                     TAAILILDEQTLLAMRTWLAGDLAGQDWATLGTSAWFSLGGFVLALYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPYELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(39023..39832)
                     /locus_tag="RH033_001792"
     CDS             complement(39023..39832)
                     /locus_tag="RH033_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV2990988.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARILQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINPLTGKPFFMPFRGVEKV"
BASE COUNT         9954 a        10727 c         9993 g         9256 t
ORIGIN      
        1 attaatcccc gtactgatta ttcttcataa tcagtacggg gttgctacaa catgtattac
       61 tctttcacca caattagcgg ttcaatatca ctctcttttt tgatgaccag tgattcatca
      121 ccgcgcaaac acgtcccacc gtagttgccg ccaacggtga aggtacatac ctgaatgtat
      181 ttaccgtcca ctttcggcaa acaccacagt tgctgataga tgtttttctg ctcggcaaat
      241 ttaccgctgg ttttgtccag cacctcttca tgatggctga cgaggtcgat gttactgcca
      301 cagcgaccgg cgatcggttt cactgcataa ccggttttca ccagttcatc attcacggag
      361 aaatcggtat ccagcaggta acgatggtgc gggaacagcg accagaggat cggcagaatc
      421 gctttgttgc cggggatcac tgtccacaga ggttcaaaga ccagcacttc cgggcgcagc
      481 aatacgtcga taagacgcac ttcattttgc ggatgaccgg tacggatcgg caccgcagca
      541 aactcacggt cgctaacttc acgaatctga tcaaacgcgg tttcccacgc ccaggttttc
      601 cacacgcagt taaccagtcg cccttcccca tcaatcagtt gcccggcagc atcccagccc
      661 agttcatcca gcccgcgcaa gatacgcgtt tcaaagcccg cctggtgcag cgcctgctcc
      721 ataaactgcg cgtgatagtt ttcctcgata tctttgtcct gcatgatatg gacaaacgga
      781 cgtgcacgac tgtgtttcca ggcaccagcc agttcgttaa tcagcccttc cgccggattg
      841 aagccgttgc ctttatagcc ctgctccgcc caacgttcga ggatcaagcc agcttcggta
      901 tgacaggagg cggagtcggc gttgtattcg tagaccttca ggccacgttc gtccatacag
      961 aaatccatac gaccggtgat catatggtga cggcgacgct gccaggagag acgcaaacgt
     1021 ggccagagga tttttgggat gtcgaacagc gccagcaggt tgtcatcttt cagcaccttg
     1081 tcggttgcgt gaagatacat caggtgcagt tcgttggtgg ctttaatcag ctcctgctct
     1141 gcgctctcgg taatggtgta gtaatgatac ggatcctgat tgatcacctg accgttggcc
     1201 tgcacatagg cgttttgtag cggatctttt tcatccagcc atttaccgtc aaactggcct
     1261 ttgttttcca ggcgcgctcc gctgattttc aacagctcgc ctgcaatttc cggctgcggt
     1321 aagctgtatt cagtatcttc cgtctggatc atccagccga gaatggtggt gtcatcaaaa
     1381 gtgtctttaa gggtatagca gccgttttcg accaccatct ccagctcgcg cgtccactgt
     1441 tgcccttgcg gtaacgggga atgaatcacg ttctgttccg caatacggac tttgttgcca
     1501 tgcaattggg taatgatggc gacatgacca gtgtctttaa attcaccgcc tttatcccag
     1561 ataagaagcg cacccgcgac cggcgcacgc ggcgagccgt taggaaatgc ctgcaatggc
     1621 aggatgttgt cattaaccac ttcacgcagg aagcgcagcg agaaaatctc ccacgccata
     1681 cccacgtcag tgaagaccac accgtaattc agaaagagaa aacggcgagc aaattcaacg
     1741 cattgccatt tgtggcccat atattcgtcg tcgatatagc tacggaatac ggcgtcatct
     1801 tcgtattcct gcggatcgag agaactgtaa tctgaagagt agattgctac cccacctggg
     1861 gcgtagccca ataatgtccc gaacggggca tcctggctgg tcgttccttt gctcatcact
     1921 ttacctttaa caatacaacc taagcagttg gcgtaattgt tgtgctctga ttacctgctc
     1981 agcgacatta accggacaga ggtcagacta atcatacacc tcatcgcagc ggctggcgca
     2041 aagggttaaa aaattcacat tctgtacagc aagtgacctg ctacactgct tcaacactac
     2101 cactcagaag gcaactcact atgacagaca acacttatca gcccgcgaaa gtctggacgt
     2161 gggacaaatc cgccggcggc gcgttcgcca atatcaatcg cccggtttct ggtccgacgc
     2221 atgaaaaaac gctgcccgtt ggcaaacacc cattgcaact ttattcgctg ggaacgccga
     2281 acggtcagaa agtaacgatt atgcttgagg agctgctggc gctgggcgtt actggtgcag
     2341 agtacgacgc ctggctgatt cgtattggcg atggcgatca attctccagc ggctttgtcg
     2401 aagtgaaccc aaactcgaag atcccggcgc tgcgcgatca tacgcataat ccgccgatcc
     2461 gcgtgtttga gtctggatcg attctgcttt atctggcgga gaaatttggc tacttcctgc
     2521 cgcaggatct ggcaaagcgt actgaaacga tgaactggct gttctggcta cagggggctg
     2581 caccgttcct cggcggtggt tttggtcact tttaccatta cgcgccggtg aaaattgagt
     2641 acgccatcaa tcgctttacc atggaagcca agcgcctgct cgacgtgctg gataagcaac
     2701 tggcgcagca taagtttgtt gcaggcgatg agtacaccat tgcggatatg gcgatttggc
     2761 cgtggtttgg caacgtggtg ttaggcggcg tgtatgatgc cgcagagttt ctggatgcgg
     2821 gaagttataa acacgtacaa cactgggcga aagaagtggg cgaacgtccg gcagtgaagc
     2881 gtgggcgtat tgttaaccgc atcaacggac cgctgaacga gcagttgcat gaacgccatg
     2941 acgccagtga tttcgagacg aatacggaag ataagcgtca ggggtaaggg ttggtattcg
     3001 tcgcagcaag ccatccaggc cggataaggc gttcacgccg catccggcaa tcgagcacaa
     3061 tgcctgatgc gacgctgccg cgtcttatca ggcctacaaa agctaatcat cacgcgctgg
     3121 taatgtcgta atccatttgg cgcagcgcgt ctaatgtggc tttggcttca tcttcgtcga
     3181 tgatgctcat ggcaaatccg acgtgtacca gtacccactg gcccagcaga tcggcagggt
     3241 taccttcaca aatcagggcg atattcacgt cgcgcttgat accacatact tcaacctgcg
     3301 caagctggtg aatatcttca ccgacagcca gcacctggcc tggaacgcca atacacattg
     3361 ttaactccgg ttattcaact tcaatacttt tgacgattag cgaatcgccg gtatcgacac
     3421 gcaaccgctc gccatgacag atcggacact gcgcatcgtg ctgatgaatc tccaccacct
     3481 ggctgcaatc ccagcaccag gcctgggcgg gtttatagac gatatgtaaa tcgcaccctt
     3541 gcgccaccgt tccgtggcag acaatttcaa aactaaaacg gacggcgctc tcctcaacgc
     3601 aggagagcgc accaatttcc agccacacgg cggtgacgcg cttaacatcg tgctgctccg
     3661 cctgccgttg gataatttca acggcgctct ggcaaagcga caactcatgc attttcgcca
     3721 ctcctgcggc caaatagcag ggcacgacgc cctgcgtgtg gtacatccgg attggtgact
     3781 ggcagcgaaa ggagcattcg ggcgcagtca tccgtcagcc gttggccctc ttcaatcgac
     3841 atgctgtgcg aaagcggcga catcagcgag caggagaggt attgcgaaac accatccagt
     3901 tcaccaacag taaaggtcat agtgccatac ggcagttgca gaccaatttt ttcactcact
     3961 ttgcgcagcg gccagagttg atccgggccg gggaagataa ctgcactcag catccacggg
     4021 gtgatcacac accccgtcca ctgaccttcg aacagcgtaa agtcagaaac atacaccggc
     4081 attgaaggat gcagaaaaga gagatcatgc atcgaacgcc gggcaatttc ttcaaaagct
     4141 gcctgtactt gcgccttcgg ggaggtctgg aaacctgcta tctcttcagt catgaatcgc
     4201 ctcccgtggg atggcttcca cgcctgattc acgcagcgca gccagaacct gctcaagcgc
     4261 aggttcaatc atcgcttcga ccgtcggcgt caagccaatg tgcggctcca gtgattccgg
     4321 gatcacgccg accagggtca gctttttcgg aaactcaccg gtgaagcgca gggccgacaa
     4381 tacgtcggcc aggccaagct gatgcggaga gattttgttg gtaaacaacg ccggaacttc
     4441 ttcatctcgc aggatcatca tcgttcccgg cgcgttcttt ttcgagacaa tggcatccgc
     4501 aataatcaga tgatcgcgat ttgccatgtc gccaagcagc tccattcccg ccgttccgcc
     4561 atcgaggatc tcaacataat ccggcagaat gtatcgttgc tctaacgctt caacaattcg
     4621 cacaccgatg gcttcatcgg tcagcaaaat attgccaacc cctaagacta aaatacgcat
     4681 tacagaacct tcactgaaac cacttcgttg ccgtcggcat ccactacgtg taccgcacag
     4741 gccatgcacg ggtcgaagga gtgaatggta cgcaccactt ccagcggttt attcggatcg
     4801 gcaaccggtg tacccaccag cgactgctcg taaggaccga cgtcatcatt gaagttacgc
     4861 gggccagagt tccaggttga tggaacaact gcctggtagt tactgataat accgtctttg
     4921 atcaccatcc agtgagagag cataccgcgc ggtgcttcaa ggaaaccaac gcctttgaac
     4981 tcacctgtag ccggaatgtt cggcttcaca aaggtggtgt gatcgccttt gccgatattg
     5041 gtgatcagtg cactgtattg attttgcagg atatcctgca attcgcagca gtgaacggta
     5101 cgaccaataa tacggcccag cgtggagtgc agctgcgcca cttccagcgt gttgccagtc
     5161 agtttctgat aaatcgcaac gatttcattc agtttgttct gggtagactc gcgtcctgcc
     5221 gccagtttca ccagcatgtt agccagcgga cccacttcta ccgttttgcc gtagaaagtc
     5281 ggtgatttca cccaggaata tttaccgtcg tcagaccaac catcataagc cggaatggtg
     5341 gtaccttccc acggtgcctg cggcgcttcg tctttatacc aggagtgctt cgcgctctcc
     5401 tgaatgcctt taatcaggta ttcatcggag tgggaagtga tcgggcgata cgaggacaga
     5461 tccgcattct caatgtagcc gcccgggaac aggaagctgc cgtttttgct gtcggtcggg
     5521 aattccggca cgctcaggta gttcaccgca cctttaccgc gcgtcagcca ttccgggtag
     5581 aacgcggcga taactgcggt atcaaccttg taaacctgct caacaaagtc gctcagttta
     5641 tcgatgaaag acttgatgta catcaggcgc tcaaggttca gcacgcccag accgtcgagg
     5701 ttgatcgggt tagcgacccc acctactgcc aggttctgaa tgtgcggcgt tttaccgccc
     5761 agcagcgcca cgacgcggtt agcgtcacgc tggcactcca gcgcctgcag gtagtgcgct
     5821 accgcaatca ggttcacttc cggcggcagt ttcatcgccg ggtgacccca gtagccattg
     5881 gcgaaaatcc ccaactgacc gctggcaacc agatctttaa tcttgttctg aactttggtg
     5941 aactcttccg gactgttcag atgccaggtc gaaacgcctt tcagcatttc agaggctttg
     6001 gttgggtcag cttgcagtgc agaagtgatg tccacccagt ccagcgccga gagctgatag
     6061 aaatgaacga tatggtcatg cgtggtgtgc gcagccagaa tgatgttacg gatgtattgc
     6121 gcgttaaccg gaacgtcgat attcagtgca ctttctgccg cacgaacgga agagagcgcg
     6181 tgagtagtcg tacatacgcc acagatacgt tgcacaatca tccatgcatc gcgcggatcg
     6241 cggtttttca cgatctcttc catgccgcgc cacatggtac cggaagccca tgctttcgaa
     6301 acgacgccat tttcgatttc gcaatcgatg cgtaaatgcc cctcaatacg ggttaccgga
     6361 tcaatagtaa ttctctggct catgctttgc tcgcctcatg acgattatga tcgttttgtt
     6421 ttaaaggagg aagtatcggc agtagacgaa tgagtacgat gtaagcgcaa atctcaatag
     6481 ccacaaaacc aatagaaatc aacagttctt cccaggtcgg gaagtaggcg taaccgccgc
     6541 ccgggttgaa tgccaccagc gaataggtca gacgccaggt tgcacaacct aacagcgcgc
     6601 tcagtgctga caggaacagc atgcgggaat cattacgcag cttcgccaca cgcagaacga
     6661 ccagcgggaa gagcatcagc aggacttcaa tccagaacat cgcggagtag aagtcaccgg
     6721 caaacgctaa cgacagcttg tcgcgataga taagctcgcc aaagcgcagc acgatgaaaa
     6781 tcgccagcaa cacactgatg gtgttggtca gcttgacgaa caggctcttt tcatctggac
     6841 cattgccacg cagacccgcc tgcaccaacg aaccttcaaa gatgacaatc gagaagccca
     6901 tgatgaacgc cgtcagcagc gagaacagcg gcaacatttc atagctctgc cacaacggat
     6961 gcactttgta gcccgccgag atcatcagcg accccattga agactggtgc atagttggca
     7021 gcagcgcacc gagcgcgata atgaagaaca tcaccttgtt cagacgctgt agcgacacct
     7081 tccagcccaa tcgttcaaac aatgccggag caaactccag tgccatcacg ccgatgtaga
     7141 tggtcataca gaccgccgtc tcgaacagta ccgagttcac gttgaagtga cccggaatgt
     7201 agaagtacgg caggttccag tagcgaccca cgtcgatagt gatcgacaag ccacccagtg
     7261 aataaccaaa cagactcgcc aacagcgccg gacgcaccag cggatggtat tgcccacggt
     7321 taaagacgta tactgcccac gccagcgccc agccgccaca ggcaaagccg gtaccgatca
     7381 gcaggtcaaa cgcgatccac acgccccacg ggaagccgcc gttcaggtcg gagacagagc
     7441 ccagaccgaa caccagacgc ttcacaatca ggagcataca gatgacgatt aacggtccaa
     7501 aaatcatgac cggtttactg atgattttgc cgcccagcgg ttgtggatca tgactcatga
     7561 tcgtctcctc cgtcgtgatg gtcgttttta gtgttgcgac gaaccagcac ggttaagccc
     7621 gccagcacag ccagtggtag catcatgcct ttatacaggg tgtgttggac atgttcggaa
     7681 cgcgcaccag tagaaagatc gtccagtttc ggcaggtcga gattttcata aggcacaccc
     7741 gtcagtacca gaacctgagt accgccgccc tctttctcgc cgtacagatg cggataatat
     7801 ttcggcaccg tatgcaggta agtgtcgcca gatttcagcg tctgacgcgg atagtggtat
     7861 tcgctgcccg gcttcagcgc cagacgtttt ttcgcctccg ccatcagctc ttcacgcgta
     7921 ccaaaaatca ccgcgcccgc cgggcacact tctacgcagc caggcagacc gcctttatca
     7981 agacgttcca cacctttctg gttgcacagt tcacacttat gcagcgcacc aaatgggttg
     8041 ttgtagtcgt acttcggcac gttgtatggg caggcgacca tgcagtaacg gcagccggtg
     8101 cacacatctt tgtcgtaatg gacaataccg gttttcggat cttttttcag tgcagagacc
     8161 gggcacacag agacacagtt cggatcgacg cagtgcatac actgtttctt aatgtacgcg
     8221 tagccattct cctcctggtc tttgttgacc cctgtgccgc tggtccacac ctgaatgatg
     8281 ttattggtat acggcgacag tttgtcgttg ttcgaccagg tctgttcccc ttgcgggtta
     8341 cgttcaggga agttgatatc ctgacacttg gtgacgcaag cctggcagcc tacgcacaag
     8401 gtcgagtcgt acaacatccc cagcgatccc ggaattggcg ggcggttttc agcagccgca
     8461 tgactgacag acggcagcgc gcccgtcagc aatgccccgc aggaggctgc tttaataaaa
     8521 ttacgtctgt tcacggttat tctccccgtg agtcagcgtt atctttcttt tgctgacgac
     8581 ccagttcacg caccgccatc acgctgacac cggcaaccag cccaaccaca ccgccgagca
     8641 aaccaatagc gcccgcagag acgttgccgc cttctttagc gttaacgtcc ggtttctgtg
     8701 aacgcggagt ctgattttcg acgttggcaa gctgatggat gcctttatgg aagccgatac
     8761 cttcttcgtt acagccatag cacgggtgac caatcgccac cggccacaca ccgccaacgt
     8821 cacagaattg cagcgttgag caattgccgt aagtttctgg ccctttacag ccgaggtggt
     8881 acaggcacca gccttcgcgg tggccttcat caccgaactc tttggcaaaa cgaccagcat
     8941 cgaagtgcgg gcgacgttcg cagtgttcgt gaatcagacg gccataggcg aaggtcggac
     9001 ggtttttgtc atccagtttc ggcggcttgc cgtaagtgat gatgtgcgca acggtcgcga
     9061 ggaagttgtg cgggttcggc gggcagcccg gaatattgat aacggttttg cctggcagaa
     9121 cttcttgcag gctgactgcg ccagttgggt taactccagc tgcggcaaca ccgccccacg
     9181 cagagcagga accgatagcg ataatggctg ctgcgccttc cgccgcttta cggatgtgat
     9241 ccacaatcgg ctcaccggca accatgcaat aaataccatt atcttttaat gggatggaac
     9301 catccaccac taacacatac tgccctttgt acttctcgag agcgttatgt ttgttctctt
     9361 cgacctgatg accgaaggcg gcggaaagca cttcgtgata ctccagagag atagtctcca
     9421 gcacgaggtt ttctaccgtt ggatgcgttg cacgaagcag agattccgta caaccggtgc
     9481 actcctgcgc gccaatccag ataactggcg gacgctgcgg gttagtaacc gattcggcca
     9541 tctctgcagc ggctttgcta cttaacccca tggtggcggc taatgctgca caaagcttca
     9601 tgaaatcacg acggttaatg ccgtgagaat ggatgagggt gttatctcca gtcatttata
     9661 gttattccgt tgcgaagacc tggcatatat tttgcctcaa tcgcaaaatc aataatgcga
     9721 tcgatgcgcc atttaccaca catttattat ggttatcgat atcatgatac tgcggcgggg
     9781 cgctaaaacg aagggaagaa gcattagtgt aattaattaa agcaagataa tacgtatgtt
     9841 tgatcaattt tcgaggcgat aaccggccat aaaaaagcgc aacgtagaac aggaattatt
     9901 ctcacgaaag aaatccatta ccatctcttt atccagtcca tagcggcgcg tcagaatacc
     9961 cgcttcccag gcgctaagct ggcgatctcc ggtcttttcg tacatccggt gtgagtagcc
    10021 taatacaaaa ccgcgtttat aatcggcaca gtattttgtc acattaaccg cactgtcggc
    10081 atgcgttgct tttaatcccg ccattaagcc tttaccaaaa tggttattca tcgttcacct
    10141 cattcgctat atattgccgt atatagcgat attttaggca aagaacgaca tttgcgcacg
    10201 ctattttttc atgcttgttt aagacgtaat cctgtaagag ataaacccat caggagagat
    10261 atttatcgaa ccatttcagc gtcctttgcc aggcaagatc ggcggcagat ttgtcataac
    10321 ggggcgtgga atcattatgg aatccgtgat taacccccgg atatatatac gcctcataga
    10381 ccttattatt ggctttcaac gccgcctcgt aagcaggcca gccctcgttg attcgggtgt
    10441 ccagttccgc gtagtgcaac agtaaaggcg cttcaatctt cgccacatcg gcagtgggtg
    10501 cctgacgacc ataaaacggc accgcgcagg ccagttccgg gtaagcgacc gctgccgcgt
    10561 tcgatacgcc accgccatag caaaatccag taatacccac tttgcctgtc gcttgcggat
    10621 agctttgcat aaactcaatc gcggcaaaga aatcattcat cagtttggtt ggatcaacct
    10681 gctgttgcag ctcacgacct ttatcatcat tacccggata acctccaacg gaacttaagc
    10741 cgtcaggtgc cagggcgata tatcccgcct tcgccactcg ccgcgtcaca tcttcgatat
    10801 acggattcag ccctcgattc tcatgcacca ctaccacggc tggcgttttg ccgctcattt
    10861 ttgcgggctt caccagataa ccccgtacct cgccgtgacc atttggcgag ggatacgtga
    10921 tgtactctgc aacaatttcc gggtcggtaa actctacctg agtcgccagc gcataatttg
    10981 gcttgagcaa atcaaacagc gctaatgccg tcatcccgcc caccgcatac ttcgccgcaa
    11041 ggttgaggaa ctcacgtttc gagattttcc cgtgagcgta atagtcgtag taatccagca
    11101 actcttgtgg gaaatctttg gcggttaaac gcggcatcgt tgcactcctc aactggtgtt
    11161 tttttaagca aagcataagc gcgtgttttt gcccagtttt tcgtcactct gtgagccaga
    11221 ctacgggata cgcgctggcg aatcgctaaa ctagaaacat tgtttcgaaa ttgaacgatg
    11281 gaaaggagag gtcatggtct ggttagcgaa tcccgaacgt tacgggcaga tgcagtaccg
    11341 ctattgcgga aaaagtggtt tacgcctgcc cgcgttatcg ctcggtttat ggcacaattt
    11401 cggtcacgtt aacgcgctgg aatcacagcg tgcgatcctg cgtaaagcgt ttgatttagg
    11461 cattacgcac tttgatttag ccaacaatta cgggccgcct ccaggaagcg cagaagagaa
    11521 ctttggtcgc ctgctgcgcg aggattttgc cgcttatcgc gatgaactga ttatctctac
    11581 caaagccgga tacgatatgt ggcccggccc gtacggctct ggcggctcac gtaaatacct
    11641 gctcgccagc ctcgaccaaa gcctgaagcg tatggggctt gagtatgtcg atatctttta
    11701 ctctcatcgc gtcgatgaaa atacgccgat ggaagaaacc gcctctgcgc tggctcatgc
    11761 ggtacaaagc ggtaaggcgc tgtatgtcgg gatctcctct tactcgccag agcggacgca
    11821 aaaaatggtt gagttgctgc acgagtggaa aattccgctg ttaattcatc aaccttcgta
    11881 caatttactg aaccgctggg tggataaaag cggcctgctg gataccctgc aaaataacgg
    11941 cgtgggctgc attgccttta ctccactggc tcagggattg ctgaccggaa aatatctcaa
    12001 cgctattccg caagattcac ggatgcatcg tgaagggaat aaagttcgtg gtctgacgcc
    12061 gaaaatgctc accgaagcca acctcaacag cctgcgctta ttgcatgaaa tggcacagca
    12121 gcgtggacaa tcaatggcgc aaatggcgtt aagctggttg ctgaaagatg agcgagtgac
    12181 gtcggtattg attggagcca gccgcgcgga gcaacttgag gagaacgtgc aggcgctgaa
    12241 taatctgaca tttagcaccg aggagctggc gcagattgat cagcatatcg ccgatggaga
    12301 gctgaatctg tggcaggcgt cttccgataa atgacctgtt aataacgggc gcgagaaccg
    12361 cgcccgaatc agatcagtga ttatgacgag tcagtcggtc aagatagccc atcacaaatg
    12421 cagagagcac aaacgtcaga tggataatga cgtaccacat cagtttgtta tcagggacat
    12481 ttttcgcatc cataaagacg cgcagtaagt gaatggaaga aattgccaca atcgacgctg
    12541 ctactttgtt tttcagcgac gttgcgtcca ttttccccag ccagttcagc ttctctttgt
    12601 tctcggagat atccaactgc gagacgaaat tctcataacc ggaaaacatc accatcacca
    12661 gcaaaccgcc aaccagtgtc atatccacca gcgacagcaa cacgaggatc aaatctgatt
    12721 ccgccatcga gaagatattc ggcagtacgt gaataatctc ctggaagaac ttcagcgcca
    12781 gggcaactaa cgccagcgaa aggccaaagt acacgggggc aagcagccag cgagaagcat
    12841 acattgcatt ttcaagaaaa cgttccatac attcctgtcg tgtagttaaa acgggcaaca
    12901 gtatatcgca attgagtaaa cagcacgcaa cagcaaatca acatatacgt taaacctttg
    12961 tcgcataaat ccgaagttta gggatcttcc tggttccctt atcaacgcat cgagagcgtc
    13021 tacccttaaa ggacgttgaa cctgaaggga gaaaaacgat gtctcattta aaagacccga
    13081 ccacgcagta ttacactggt gaatatccca aacagaaaca accgacgcca ggcatccagg
    13141 cgaagatgac accggtaccg gattgcggcg agaaaaccta tgttggtagc ggtcgcctga
    13201 aagatcgtaa agcactggtg acagggggcg attccggaat cggtcgcgct gccgccattg
    13261 cttacgcgcg tgaaggggct gacgtggcga tcagttatct tcccgtagaa gaacaagacg
    13321 ctcaggatgt gaaaaagatc attgaagaat gcggacgcaa agccgtcctg ctgccaggcg
    13381 atttaagcga tgagaaattc gcccgttcgc tggttcacga agcgcacaag gcgttaggcg
    13441 ggctggatat tatggcgctg gtcgccggga aacaggttgc cattactgat attgcagacc
    13501 tcaccagcga acagtttcaa aagacctttg ccattaacgt tttcgcgctg ttctggctaa
    13561 cccaggaagc gatccccctg ctaccgaaag gtgccagtat catcaccact tcgtcaatcc
    13621 aggcatacca gccaagcccg catttactgg actatgcggc tacgaaggcg gcgattctga
    13681 actacagccg tggcctggca aaacaggtcg cggagaaagg tattcgtgtg aatattgtcg
    13741 cacctggccc gatctggaca gcactgcaaa tttccggcgg gcaaacgcag gataagatcc
    13801 cgcagtttgg tcagaaaacg ccgatgaagc gtgcggggca accggcggaa ctggcccctg
    13861 tatatgttta tctggcaagt caggagtcga gctacgtcac cgcagaagtg catggcgtgt
    13921 gcggcggcga gcatttaggc taaaaaaatg cccggttgtg aaaagcaacc gggcatcatt
    13981 tttatttcgc tttcgcagtt tcttcgccga ccagacctat cttcaggtaa cccgcctgat
    14041 gcagcgtatc cattaccttc atcaacgtct cgtaatcgac ggttttgtcc gctcggaaga
    14101 agatggtggt gtctttcttg ccttcggtta acgcattcaa cgccgtaatc attgtttcat
    14161 cggtgaccgg atcgttaccg ataaacatcg agttgtctgc cttcaccgac agataaaccg
    14221 gtttttccgg gcgcggctgc ggcgtgctgg tagaagcagg caagttcacc ttcacatcta
    14281 ccgtcgctaa cggtgccgcc accataaaga taatcagcag aaccaacatc acgtcgataa
    14341 acggcgtcac gttgatatca tgcatttcgc cgttatcgtc gaggttttcg ttaagatgca
    14401 ttgccatcgg acattatcct gcgcgtaatt tttgtgcgac acgaaccgga tgcgcagcgg
    14461 cgctggcttc cagatccagg tcacggcttt gcagcaacaa tacctgcgct gcaacatcac
    14521 ccagcatcgc tttaaagccg ccaatctggc gtgcaaatac gttatagata acgaccgccg
    14581 gaatcgctgc cacgaggccg attgccgttg ctaacagagc ttctgcgata cccggcgcaa
    14641 cgactgccag gttagtagtc tgcgtttgcg cgataccaat aaagctgttc atgatgcccc
    14701 agaccgtacc aaacagacca acgaacggcg aaatcgcgcc gatggttgcc agatagccgt
    14761 taccacggcc catttgacga cctactgcgg cgacccgacg ctccagacgg aagctggtac
    14821 gttctttaat accttcgtta tcgtcgctgc cttctgacag ttccagctca ttctgcgctt
    14881 cattgagcaa atgcaggctt aagcttttgc taccaaaatc agcggcgata tcgttggcct
    14941 ggtttaagga acgcgcttca gccagcagtt gctgctcgcg cttaaggcga cgcttctgat
    15001 tgaagaactc tacgctctta ctgaagaaga ttgcccaggt gactacggag gccaaaataa
    15061 gcccaatcat cacgcactta acgacaatat cggcgtgctg atacataccc cagacggaaa
    15121 ggtccgtctg cattaaatta ttacccactg tgtatctcca ggacgcaagt cacaaaatct
    15181 gcgcataata atatcaaaac gacgtcgaat tgatagtcgt tctcattact atttgcatac
    15241 tgccgtacct ttgctttctt ttccttgcgt ttacgcagta aaaaagtcac cagcacgcca
    15301 tttgcgaaaa ttttctgctt tatgccaatt cttcaggatg cgcccgcgaa tattcatgct
    15361 agtttagaca tccagacgta taaaaacagg aatcccgaga tggcggacaa aaagcttgat
    15421 actcaactgg tgaatgcagg acgcagcaaa aaatacactc tcggcgcggt aaatagcgtg
    15481 attcagcgcg cttcttcgct ggtctttgac agtgtggaag ccaaaaaaca cgcaacgcgc
    15541 aatcgcgcca atggcgagtt gttctatgga cggcgcggaa cgttaaccca tttctcctta
    15601 caacaagcga tgtgcgaact ggaaggtggc gcaggctgcg tgctgttccc ctgcggggcg
    15661 gcggcggttg ctaattctat tcttgctttt gtcgaacagg gcgatcatgt gttgatgacc
    15721 aacaccgcct atgaaccgag tcaggatttc tgtagcaaaa tcctcagcaa actgggcgta
    15781 acgacatcgt ggtttgatcc gctgattggc gcagatatcg ttaaacatgt gcagccaaac
    15841 actaaaatcg tgtttctgga atccccaggc tccatcacca tggaagtcca cgacgttccg
    15901 gcgattgttg ccgccgtgcg caatgtggtg ccagatgcca tcattatggt cgataacacc
    15961 tgggcggccg gtgtgctgtt taaggcgctg gattttggca ttgatgtttc cattcaggcc
    16021 gctaccaaat atctggtggg gcattcagat gcgatgattg gcactgccgt gtgcaatgca
    16081 cgttgctggg agcagctgcg ggagaatgcc tatctgatgg gccagatggt cgatgccgat
    16141 accgcctata taaccagccg tggcctgcgc actttgggtg ttcgcctgcg tcaacatcat
    16201 gaaagcagtc tgaaagtggc tgaatggctg gcagaacatc cccaagtagc gcgagttaac
    16261 caccctgctc tgcctggcag taaaggccac gaattctgga aacgagactt tacaggtagc
    16321 agcgggctat tttcctttgt gcttaagaaa aagctcaatg atgaagagct ggcgaactat
    16381 ctggataact tcagtttatt cagcatggcc tactcgtggg gcgggtatga atcgttgatc
    16441 ctggcaaatc aaccagaaca tatcgccgcc attcgtccac aaggcgagat cgattttagc
    16501 gggaccttga ttcgcctgca tattggtctg gaagatgtcg acgatctgat tgccgatctg
    16561 gacgccggtt ttgcgcgaat tgtataacat tgccactttt ggacaatttt gcagacattt
    16621 tattgtgaaa agtcttaaat tgttgcgtcc gggatcaagg cgtcccggac gattcaggag
    16681 tacaataggc agataaaggc ttaaacgctg ttccacagga aagtccatgg ctgttattca
    16741 agatatcatc gctgcgctct ggcaacacga ctttgccgcg ctggcggatc ctcatattgt
    16801 tagcgttgtt tactttgtca tgtttgccac gctgttttta gaaaacggcc tgctgcccgc
    16861 ctcatttttg ccaggcgaca gcttgttgat attggctggc gcattgattg cccagggggt
    16921 tatggatttt ctgcctacga ttgcgattct gaccgccgca gcaagtctgg gctgctggct
    16981 aagttatatt caggggcgct ggttagggaa taccaaaacg gtgaaaggct ggctggcaca
    17041 gcttcctgct aaatatcacc agcgcgccac ctgcatgttt gaccgccacg gtctgctggc
    17101 gctgctggct ggacgttttc ttgcatttgt ccgtacgctg ctgccaacca tggcaggaat
    17161 ttccggtttg ccaaaccgcc gtttccagtt tttcaactgg ttaagcggat tgctgtgggt
    17221 cagcgtggta accagtttcg gctatgcctt aagtatgatt ccgttcgtta aacgccatga
    17281 agatcaggta atgacgttcc tgatgatcct gccaattgcc ttgttaaccg ctggcttgtt
    17341 aggcacgctg tttgtggtga ttaaaaaaaa atactgtaac gcctgacgat tttccccgtt
    17401 cccggttgct gtaccgggaa cgtatttaat tcccctgcat cgcccgcatt cttgccgcat
    17461 cttcccccgg cgtcacacca aagtaacgtt taaactcacg gctaaattgc gatgcgcttt
    17521 cgtaaccgac gcgcatcgct gccgcactgg ctttcatgcc gtcgtggatg atcatcatcc
    17581 gcgccttatg cagacggtaa ttcttcaaat actgcaacgg cgaggtgctg gtgacagact
    17641 taaaattatg gtggaacgcc gatacgctca tgttggcttc tgccgccagt tgctcgacgc
    17701 tcaggttttc ggtgtattta ttctcaatcc gtttcagcac gcgactaatc aggctgaagt
    17761 gagtctggcg gctgaccagc gccagtaacg cgccgccgca aggtccggtc agcacgtagt
    17821 acagaatttc gcggatgatc tgtttgccga gaatacgcgc atccagtggt cgctccatca
    17881 cgtcgagtaa ccgctccgcc gcgcataaaa tctcttctga taacgtggca gagttaatcc
    17941 cgctggctgc catcgacggc tggaaatgct catcttcgcc aatgtccatc aacagttcct
    18001 gtaactgcaa aatatcgaaa ttgagacgca accctgccag cggcacctct gacgtggcat
    18061 aggtttcgca ctcaaacggc aacggcaccg tcagcagcag gtattcattg gcatcataac
    18121 gaaacacgcg ttcattgata taaccgattt tatgcccgga aaagagaatt atgatgccag
    18181 gctcgtacat taccggtgta cgtgcgaaag gcgtctcgcc atacaacaaa cgcacatcgg
    18241 gcaacagccc tgacaaacaa ttttctttat ttttcagttt attaacttta tccgccagca
    18301 agcggcaaat ctcttcacgt ttcatatcgc gtaatttctt aggaataatg cggcaatttg
    18361 attgtgcgca attttgtagc atttctccag cactctggag aaataggcaa gacattggca
    18421 gaaatgagca ttgagagcca gggcgctggc gatcacaatg aaaaacatca ggcagatcgt
    18481 tctctgccct catattggcc cagcaaaggg agcaagtaat gaacaacttt aatctgcaca
    18541 ccccaacccg cattctgttt ggtaaaggcg caatcgctgg tttacgcgaa caaattcctc
    18601 acgatgctcg cgtattgatt acctacggtg gcggcagcgt gaaaaaaacc ggcgttctcg
    18661 atcaagttct ggatgccctg aaaggcatgg acgtgctgga atttggcggt attgagccaa
    18721 acccggctta tgaaacgctg atgaacgccg tgaaactggt tcgcgaacaa aaagtgactt
    18781 tcctgctggc agttggcggc ggttctgtac tggacggcac caaatttatc gccgcagcgg
    18841 ctaactatcc ggaaaatatc gatccgtggc acattctgca aacgggcggt aaagagatta
    18901 aaagcgccat cccgatgggc tgtgtgctga cgctgccagc aaccggttca gaatccaacg
    18961 caggtgcggt gatctcccgt aaaaccacag gcgacaagca ggcgttccat tctgcccatg
    19021 ttcagcccgt atttgccgtg ctcgatccgg tttataccta caccctgccg ccgcgtcagg
    19081 tggctaacgg cgtagtggac gcctttgtac acaccgtgga acagtatgtt accaaaccgg
    19141 ttgatgccaa aattcaggac cgtttcgcag aaggcatttt gctgacgctg atcgaagatg
    19201 gtccgaaagc cctgaaagag ccagaaaatt acgatgtgcg cgccaacgtc atgtgggcgg
    19261 cgactcaggc gctgaacggt ttgatcggcg ctggcgtacc gcaggactgg gcaacgcata
    19321 tgctgggcca cgaactgact gcgatgcacg gtctggatca cgcgcaaaca ctggctatcg
    19381 tcctgcctgc actgtggaat gaaaaacgcg ataccaagcg cgctaagctg ctgcaatatg
    19441 ctgaacgcgt ctggaatatc actgaaggtt cagacgatga gcgtattgac gccgcgattg
    19501 ccgcaacccg caatttcttt gagcaattag gcgtgccgac ccacctctcc gactacggtc
    19561 tggacggcag ctccatcccg gctttgctga aaaaactgga agagcacggc atgacccaac
    19621 tgggcgaaaa tcatgacatt acgctggatg tcagccgccg tatatacgaa gccgcccgct
    19681 aagcttttta cgcctcaaac tttcgttttc gggcatttcg tccagactta agttcacaac
    19741 acctcaccgg agcctgctcc ggtgagttca tataaaggag gaacgtatgg ctaatccaac
    19801 cgttattaag ctacaggatg gcaatgtcat gccccagctg ggactgggcg tctggcaagc
    19861 aagtaatgag gaagtaatca ccgccattca aaaagcgtta gaagtgggtt atcgctcgat
    19921 tgataccgcc gcggcctaca agaacgaaga aggtgtcggc aaagccctga aaaatgcctc
    19981 agtcaacaga gaagaactgt tcatcaccac taagctgtgg aacgacgacc acaagcgccc
    20041 ccgcgaagcc ctgctcgaca gcctgaaaaa actccagctt gattatatcg acctctattt
    20101 aatgcactgg cccgttcccg ctatcgacca ttatgtcgaa gcatggaaag gcatgatcga
    20161 attgcaaaaa gagggattaa tcaaaagcat cggcgtgtgc aacttccaga tccatcacct
    20221 gcaacgcctg attgatgaaa ctggcgtgac gcctgtgata aaccagatcg aacttcaccc
    20281 gctgatgcaa caacgccaac tacatgcctg gaacgcgaca cacaaaatac agaccgaatc
    20341 ctggagtcca ttagcgcaag gtgggaaagg tgttttcgat cagaaagtca ttcgcgatct
    20401 ggcagataaa tacggcaaaa cacctgcgca aattgttatc cgctggcatc tggatagcgg
    20461 cctggtggtg atcccaaaat cggtcacacc ttcacgcatt gtcgaaaact ttgatgtctg
    20521 ggatttccgt ctcgacaaag acgaacttgg cgaaattgca aaactcgatc agggcaagcg
    20581 tctcggtccc gatcctgacc agttcggcgg ctaacgtgca aattctcccg gtggcggtaa
    20641 tgttccgcta ccggactttt cagaaatcat ttattcccct cgcgtcccgc ccgttgctac
    20701 tcttccttgt tcaggaatgt caaatataag gacatcatca tgcagagccg gaagctctta
    20761 aaagaacaac tcatctatat ccgggataaa cgcaacggag aggtgaaaaa cagatgaaaa
    20821 taatacttct gtttttagca gccctggcaa gttttaccgt acacgcacag cccccctcac
    20881 agaccgtaga acaaacagtc cggcatattt atcagaacta taaatcagat gccactgccc
    20941 cttattttgg tgaaaccgga gagcgagcga taacttctgc gcgtattcaa caggcgctta
    21001 ccctgaacga caatcttacg ctgccgggca atattggctg gctggattat gatccggttt
    21061 gtgattgtca ggattttggc gatctggtgt tagaaagcgt agcgataacc caacctgacg
    21121 ccgatcatgc cgatgccgtt gtgcgctttc gtatctttaa agacgataaa gaaaagacca
    21181 tgcagacact gaaaatggtg gcggaaaatg gtcgttgggt cattgacgat attgtcagca
    21241 atcatggcag cgtcttacaa gcagttaata gcgagaatga aaaaacgctg gccgctatag
    21301 cttcgttgca aaaagaacag ccggaagcct ttgttgccga actctttgaa catattgctg
    21361 attatagctg gccgtggacg tgggtggttt ccgactctta ccgccaggcg gtcaatgcct
    21421 tctataaaac caccttcaag actgccaaca atcccgatga agatatgcaa atagaacggc
    21481 aatttattta cgacaatccg atctgttttg gcgaagagtc gctattttca cgtgttgatg
    21541 aaatccgtgt cctggagaaa accgccgatt ccgcccgcat tcatgttcgt tttacgctga
    21601 ccaatggcaa caacgaagag caagaactgg ttttacagcg gcgcgaaggg aaatgggaaa
    21661 tcgctgattt tatccgcccg aacagcggca gcctacttaa acagattgag gcaaaaaccg
    21721 ccgccagatt aaagcaatga gctgaattaa ataacaatta gccggaacaa taaataaaag
    21781 ggaacactat atgaaaacga ttttcaccgt gggagctgtt gttctggcaa cctgcttgct
    21841 cagtggctgc gtcaatgagc aaaaggtcaa tcagctggcg agcaatgtgc aaacattaaa
    21901 tgccaaaatc gcccggcttg agcaggatat gaaagcatta cgcccacaaa tctatgctgc
    21961 caaatccgac gctaacagag ccaatacgcg tcttgatgca caggactatt tcgactgcct
    22021 gcgctgcttg cgtatgtacg cagaatgata aaaaaatccc cggcagcatt ctgttaccgg
    22081 ggatattttt taacgtccaa ccgccgcttt agggcgtttc ttcgcaccag cattctgcag
    22141 gcgagattgc gtagacgacg ctttttttgc cgtagcaggc gtctgacgct gggtcgccat
    22201 cggcgtatgt ttagtcaacg ccggacgggt attgcggttc tgacggcgag cttcacgcat
    22261 ctcttcaatg gttggcgcag gcactaagca atcgcgacgg ctgccaatca gatgcttttt
    22321 gcccatcgct tccagcgcct ggcggattaa cggccagttt gccggatcgt ggtaacgcaa
    22381 caacgcttta tgcaggcgac gctgcttgtc gcccttaggt acgaagacgt cttcactctt
    22441 ataaccaatc ttcgccagcg ggtttttgcc ggtgtaatac atggtggtcg agttagccag
    22501 cggcgacgga tagaagttct gcacctggtc gaggcggaag cgatgctttt tcagccatag
    22561 cgccagattc accatatctt catcacgcgt acccgggtgc gcggagatga aatacgggat
    22621 cagatactgt tctttacctg cctgtttcga gtaagtatcg aacagctctt taaagcggtc
    22681 atagctgccc atgcccggct tcatcatctt cgataacggc ccttcttcgg tatgttccgg
    22741 ggcaatcttc agataaccgc caacgtgatg ggtcgccagc tctttgatat agcgcggatc
    22801 ttctacggcg atgtcataac gcacaccaga ggcaatcagg atctttttaa tgcctttcag
    22861 atcgcgcgca cggcgataga ggttgatcgt cggttcgtgg ttagtgtcca tatgcggaca
    22921 aatatccgga taaacgcacg acaaacgacg gcaggtttgc tcagcgcgtg gcgatttgca
    22981 acgcaacata tacatgttgg cagtaggccc accgagatcg gaaatcacgc ccgtaaaacc
    23041 tggaacagtg tcgcggatcg cttcgatctc attgatgatc gaatcttccg aacggctctg
    23101 aataatgcgc ccttcatgct cggtaataga acagaaagaa cagccaccaa agcagccacg
    23161 cataatgttg accgagaaac ggatcatttc gtaagccgga atacgagcat tgccataggc
    23221 cggatgcggc acgcgcttgt acggcagcgc aaaaacgctg tccatctctt cggtagagag
    23281 cggaatagca ggcgggttga tccacacata gcggtcgcca tgtttttgca tcaatgcgcg
    23341 ggcgcagcct gggttagttt cgtggtgcag aatacgcgaa gcatgggcgt acagcacttt
    23401 atcgcccttc actttctcga aagaaggcag caatacgtag gttttttccc acggtttcgg
    23461 gcgcggcggc tgcacggtta cggctttcgc ttcctgcttt ttcggcgcaa caggtttgtt
    23521 atccgcgcac ggcagatctt cgccatacgg atgcgggatc gggtcgattt tcccaggagt
    23581 atcaagacgg gtggaatcca cgccgctcca gcctggcaac gcctctttta cgataatcgc
    23641 ggtattacgc acatcgcgga tttcactaat cggctcgccc atcgccagac ggtgcgccac
    23701 ttccaccagc ggacgctcac cgttaccaaa catcagcatg tcggctttcg aatccaccaa
    23761 cacggaacgg cgcacggtat cggaccagta atcataatgc gcggtacggc gcaggctggc
    23821 ctcaataccg ccgaggatca ccggcacatc tttccacgcc tctttacaac gttgggtata
    23881 aaccagtgtg gcgcgatccg gacgcttacc cgcgacatta tctggcgtgt aggcatcgtc
    23941 atgacgtaaa cggcgatcgg cagtgtaacg gttgatcatc gaatccatgt tgcctgcggt
    24001 aacgccgaaa aagagattcg gtttacccag acgcatgaaa tcgtctttgc tgctccagtc
    24061 tggctgggcg atgatcccga cacgaaagcc ttgcgcttcc agcatacgac cgcaaatcgc
    24121 catcccgaag cttgggtgat caacatatgc atcacctgtt accaaaatga tgtcgcagct
    24181 atcccagcca agttgatcca tctcttcacg agacatcggc aaaaacggtg ccggtccaaa
    24241 acaggcggcc cagtactgcg gccaggagaa caggtcgcga tccggttgga tcagggagat
    24301 agagctcatt ttgcttccag aaatgataaa aaaataatca aaggccgggg attataagcc
    24361 ggaacgaaag agaaatcgaa aggtattcca tactcgccct cctcgggcga gtatgaagat
    24421 tacggtaccg gattgactaa cagttgccct atcgagccac ggtccgccat ttccagcgtc
    24481 tgactgttga agtagaacgg gaagtgcgcc caggaaggct gaccgaaata aaccagcagc
    24541 tccacttgtc cgtcaaccca aacggtatct ttccagcctc tgtcctccgg gaacggcatc
    24601 gcgccattca cgttacggat ctggaacatc acgccttcaa tatggaacgc ttgcggctcg
    24661 tccgcgcgta ccgtccagcg ttcccacgtt ccttgctgcg cggtgacatc aatacggtta
    24721 acgtcccaca gttgtccatt aatacccggg tcatcaccca gactgatatc acgactgcga
    24781 atcggcgaac cagccatgat ttcagttggc agcaagcgca tcggaagact gtctgtgacc
    24841 agcggcagaa ggccggttgg gcgcagcgtt agcaccagcg tagaaaccag aatgctggat
    24901 ggctcaaaga agccacgaat acgatcaaca atgctcgccg cttcgccaca ggtgatcgac
    24961 acttcatcac cgttgctcat atccaccaga atctcacgac gctcgcccgg tgccagcgaa
    25021 agttgcttca ccgatacagg agcgggcagg aatccctgat cgccagaaat cacatgtaac
    25081 gggcgaccat cgctcatctg tagttgatag cgacgagagt tcgacgcgtt cagcagtcgc
    25141 aagcgcaccc agcctcgcga gacttcaacg tacgggcttt gtacaccgtt aaccagcagt
    25201 gtgtcaccaa caaagccgcc gcttcccggt tcgttgtatt ctggcgtacc aaagttgtcc
    25261 agccgtttat cctggataat gaccggaaaa tcatccacac cataatggtt ggggataggc
    25321 agcgacttgc tgacttcatc ttccaccagc cacattccgg caaggccgtt atagacctgc
    25381 tgagccgtgc ggttgggagt attggcgtga taccacagag tagctgcgtt ctggcgaatg
    25441 ggcagtacgg gtgcccagtc agcgtttggc gacatcatcc gtgccggacc gcccatcagc
    25501 gggcctggta cctgtagccc ggccaccgtc attgagacat tttctgtcag gcggttgctg
    25561 taaataagct taacatcgtc gcccttccat acgcggatag tcggccccag gtaacgacca
    25621 ttgattcccc aaaccgacgc gcgtgtccct ggcgtaaatg accagtgcgc acgttgtaca
    25681 gtcataaaca gcggttgccc acggcgagat tcaagcagcg gcggaacggg tagcggttgc
    25741 tgttgcccgg ctgcgctggc cttcagggga acagcgcctg cacaaagtgc aatccccgat
    25801 gcctgaatga actgacgccg actgagtgac atatttgctc catgtaaaac tggctaaagt
    25861 aataccgtac aggaaagtgt tccccgcaaa aatacagatt gccgacttaa acttttccgg
    25921 cggcttcgcg ctctgcgact tctttatcga gctcagcgat tttttgttcc attatcgaac
    25981 gacaatgggc agccaactcg cgaacctgat ctttgccata ctgactgacg tcaattggcg
    26041 gcagcatttc gacaatcacc agaccgttgt gtagccgatt aagattaatc ttattcgaag
    26101 ttgtagagac gcacacggga ataatcggga cgcccgccgc aattgccgcg tgaaatgccc
    26161 cggtcttgaa cggtagcagg ccgcgaccac ggctgcgggt tccttccggg aacatccaga
    26221 tggaaatacg gcgttttttg aagtgattca cgacttccgc aatagtgccg tgagctttag
    26281 tgcgattgtt tctgtcgatc aataagttgc cggttaacca gtacaactgc ccgaagaagg
    26341 ggatccacag caagctcttt ttccctaccg tcaccgtcgg cggttgcaca atgttcgatg
    26401 ctgtcaccat gtcatagttg ttctggtggt tagcgatata gatagcattg ccgtagcttt
    26461 cagcatccgc aggtttgcgg cactcaactt tcaggccgaa cagcggtgca agacgaccaa
    26521 acatatgccc aaaggtggcc acatgtttcg ggttacgcgg gctgaaaagg cagtaaatgg
    26581 agccgaatac acagactaag atgctgtaaa tcacggtaat aataagacga aagatatata
    26641 gcataacacc ctctgaaggt ctgagagact ggcattgtac gtcacctgaa atcaggttag
    26701 ggcattgtta aaagccgctc tctggaaaag cgtattgtta atcgcaacgc atgaataaac
    26761 aacggtttca agggaatttt tatcgtttcc gggggttgaa tacgctgccg gatgacgact
    26821 taacgtttca tccggcgttc cttgcaagcg ggaggaaaca gcgccctccc cggcatatta
    26881 ctcttcgcta tcaccgctgc tggcacggcg aggagagtcg atctcaacac gatcgatacg
    26941 ctgcaaaccg cgcatcaacg taccgcggcg tccacgttcg ccagtgactt tctgtagctc
    27001 ttccggacgc agtttaattt tgcgtttccc aacatgaatg gtcagcgtgc tttgcggcgg
    27061 cagaacgtac agttgcgcaa gaccatcctc gccacgcgcg gcttctgccg atggaatgtt
    27121 gataatcttg ttgcctttgc ccttcgacag ctgcggcaga tcgcttaccg ggaacatcaa
    27181 catacggcct gcctgagtga ttgccagcag catatcggaa gcatcttcaa tcaccaccgg
    27241 cggcataaca tgggcatttt ccggtaaggt gatcaaagct ttacctgcac ggttacgcgc
    27301 caccagatcg ttaaaggtgc agacgaaacc gtaacccgca tcggaagcca tcagcagttt
    27361 ctgatcgtcg ctttccatca gcatatggtc aacggtcgct ccaggcggca acgttaattt
    27421 gccggtgagc ggctcgccct gaccacgcgc cgacggcagc gtaatcgggt cgatggcata
    27481 gctacgaccg gtggaatcaa caaacactac cggttggttg ctcttacctt tcaccgccgc
    27541 tttgaagcta tcgcccgctt tataattcag gcccggcgcg tcgatatcat ggcctttagc
    27601 gctgcgtacc cagcccatct gcgacagcac aatggtgaca ggttcagacg gcagcatgtc
    27661 gtgctcgctc atcgctttcg cttcttcgcg ttcctgcaac ggcgaacgac gttcgtcacc
    27721 gtaggcttgc gcgtctgcct gcagttcttt cttcagcagg ttattcattt tacgctcgga
    27781 agccaaaatg ccctgcaact ggtcgcgctc tttttccagc tcactctgct caccgcgaat
    27841 cttcatctct tccagtttgg caagatgacg cagtttcagt tcgaggatcg cttccgcctg
    27901 ggtttccgta aggccaaacc gcgacatcag cgccggtttc ggttcatctt cattacgaat
    27961 gatctcaatc acttcgtcga tattgagaaa cgccaccagc aaaccttcga ggatatgcag
    28021 gcgcttgagg actttctcca gacgatagtt cagtcggcgg cgcacggtat cgcggcggaa
    28081 caccagccat tcggagagga tttccagcag gtttttcacc gccggacgac catccagacc
    28141 gatcatattg aggttaatgc gatagctctt ttccagatcg gtggtagcaa agaggtggtt
    28201 catcacctga tccatatcca cgcggttgga acgcggcaca atcaccagac gggtcgggtt
    28261 ctcgtggtca gattcatcgc gcagatcgtc aaccatcggc agctttttgt tgcgcatttg
    28321 cgcagcaatt tgctccagta cgcgcgcacc tgaaacctga tgcggcaatg cgctgataac
    28381 caccgcgcca tcttctttct tccaaaccgc gcgaatacgc actgaaccac gtccgttctc
    28441 gtagatttta cgaatctcgg cgcgcgaagt gataatttcc gcttcagtcg gataatccgg
    28501 cccctgcacg atatccagca gctgatcgag cgtggttttc ggctggtcga ttaaggcgat
    28561 tgccgcctga gccacttcac gcaggttatg cggtggaata tcggtcgcca tgccgacggc
    28621 aataccggtg gtgccgttaa gcaaaatgtt tggcagacgg gcaggtagca ttttcggctc
    28681 ctgcaaagtg ccgtcgaagt ttggtaccca gtcagccgtc ccctgcccca gctcgctcaa
    28741 tagcagctcg gaatatttcg acaaccggga ttcggtgtaa cgcattgccg cgaacgattt
    28801 cggatcgtcc ggcgcgcccc agttcccctg accatcaacc agcggataac ggtaagagaa
    28861 cggctgcgcc atcaggacca tcgcttcata acaggcacta tcgccgtgcg gatggtattt
    28921 acccagtacg tcaccgacgg tacgggccga ttttttaaat ttggcgctgg cattcaggcc
    28981 cagctcagac atcgcataca caatgcggcg ctgaacaggt ttcagaccat caccaataaa
    29041 cggcaacgca cggtccatga tcacatacat ggagtagttt aagtaggcgt tttccgtaaa
    29101 ttcatgtagc gcaaggcgct ctgccatatc gctcattaat tctgattcct caacttattc
    29161 gccagccttc aaaggggcaa tattgccgga gatactacat tatctgccgc gtcgagtcac
    29221 aggagatcag cgtgaatagc gccgccggac gtgctccgac ggcgataaag acatcaatgt
    29281 gtcttgatat acaactcttt gctgtagaca taatcctgag gattatgcag cggaaaaccg
    29341 ccaacgtagg gtttaagcag tttgattaac ggctgatagt agatgggaat cagcggtgcc
    29401 tgctggttga tgatcacttc tgcctgctga tacaacgcat tacgctttgt cgcatcagtg
    29461 atctgcgtgg cctggtttag taaggcgtca tactgcgcat ttttccagtg accgacgttt
    29521 tcttcaatat cgcttttgag cgtgttcagg aagctggaag catcattgta cgtcgcatcc
    29581 cacgactgcc gcgacaacat gaaatcaccg gttcgtcggg catcaagata ggttttccac
    29641 tccattgtgc gcagcgtcac ctgtgcaccc agccattttt tccattcgga agacaacgct
    29701 atcgcggtct tttcatgcag atcgtacttg ttgtagaaca gctcaaagcg tagcggatga
    29761 gaggcgtcgt atcccgcctg tttcagcaag gcttttgcca tcgcgacacg ctcactcatt
    29821 ggcttttgca gttcatcgaa cgtcgtcgcg ctaaagcctt ttacctctgg cggcgtcagc
    29881 gtggttgcgg gcgttctcaa ccccagtacc ttttgcgcaa taagctgtcg atcaaccgta
    29941 agatatagcg cccgacgcac ccgcacatcg ttaaatggcg gtttctcaag gttaaagttg
    30001 taatattcgc tgttcagacg cgggataatt cgtagctcgc caggcagtga tttttcaatg
    30061 gcaggaattt gctgcgccgg aacccaggtg agatcgacct ctcccgcgcg atagcggtta
    30121 tagccggtga ccgaattatc cagcgccaga tactcaacct gttgcaatac tgtatgttgc
    30181 gcatcgcggt actttggatt tttgcgtgca gtaatctttt cgttaactac ccactgatca
    30241 agcacaaagg caccgttgta aaccatgttc tctggcttac tccagctatc gccatgttta
    30301 gcgatgacat gatgaggaac cgggaacaac gtcggccagg cgagcatcgt cgtaaaccaa
    30361 ggaactggct gctcaagcgt aacttcaaga gtacgatcat ccgtcgcttt gatccccagc
    30421 gatgtaacat ctgctttacc cgcaacaata gccgcggcat tgttaatgtg cgcctgtgcc
    30481 agatagccag caaaagggct tgccgttttc gggtcaaccg cacgttgcca gccgaggaca
    30541 aaatcctctg ccgtcagagg ctgaccgtct gaccactgca aaccgctacg tagatggaaa
    30601 atatagcgct tgccgccgtc cagtatctcc cagcgttcag cctgagcggg ctgcacctgg
    30661 ccttcaccgt ccatccatac cagaccttca aacagatcca gcacaatctg cgcggcagta
    30721 ttctcctcta ccttttgcgg gtcgagcgtg cctgggtcgc tatgattgtt gtaacgaaaa
    30781 acttgttgcg gggcgagcgg tgtgttggcg ggaacgtcag cagcgtagag aggagccaca
    30841 cttaccaggc tgaccagcca taataaattt cgcgtataca tggtattcct taccggagaa
    30901 attataagcc cgacaacaat acgcctgtgg cattgttagc tcaaacttat cgcgagtgat
    30961 ttggctcaca ctccggtaaa gattccccat cacgctaacg gtacataaat atccgtttcc
    31021 agtcgccgct ctgtcatccc ttcggcaaga ttggtgtagt gaaacaggat cggggctttg
    31081 cgcatttttt cgccgcttgc aggtagccag tggcgaatga tgccccatat cgtgtgtgaa
    31141 atatcgtcca gctcgccaac atggcgggca acagcataac gcccaccggt aagttcgcca
    31201 ttactcacgc cataacgatt atcggggatc ggttcgctaa cgctgccgca gatatcaaag
    31261 cgaaacgctt ctggtgcggt ggtggctggg tcgtcccagg cgacgccaaa agtctggctt
    31321 tggttaacag gagaaagccc cgtctcttta cgccacatga taaatttcgc cacgctgtag
    31381 ttgagcaact cagggctaca gcggtgcggc aacatcgcca cagggattga tgggaaatcg
    31441 ataatattta catccagtgt caggtttgtc attttgcctc cctgcgaaat tattcccggg
    31501 aacgcgtccc gggagcggta acaattacgg atttactttg cggatctgtt tgacgtcaat
    31561 ttcaacagag ttccagtctt tatcgacttc accctgaatc tcaactgtat ctttcggcgt
    31621 caccgtcacg ccgttccagc gtttgtggtc gatatcaaca ttgatagtac cgctggcatc
    31681 tttgaacacg tagagatcgt cagagatgcg ttcaacgata ttgccgcgca gggttaccca
    31741 ggtgtcgtca cgcagggatt ttgcgctttc tacggtcgtt acgctgccgt tcggcccctg
    31801 gaatcctccg gcctgacttt gcgttgccga tgggccagaa aaaccgccct gctctgctgc
    31861 catcaccggt gcgctgcaca gggccattac tgcgattact gctgcgaatt ttttcatgtt
    31921 tattactccc tttaatgtct gtttccgagc atttaacaag atagtcctta acaacttctt
    31981 aagggaaaaa aataaaattt aatgctgtac agagcgcgtt acaacacggt ttactggcag
    32041 caaatacggt tatcgcaggg atgaaaaaat gcgaatttta ctgatagaag atgacatgct
    32101 gattggcgac ggcatcaaaa cgggccttag taaaatgggt tttagcatcg actggtttac
    32161 acaaggtcgt cagggaaaag aggcgctata tagcgcgcct tatgatgcgg tgatcctgga
    32221 tttaacctta ccaggcatgg atggtcgcga tattttgcgc gaatggcgag aaaaaggtca
    32281 gcgtgagccg gtactgatcc tgaccgcgcg cgatgcgctg gcggaacgtg tagaagggct
    32341 gcgtctggga gctgacgatt atctgtgtaa accttttgcg ttgatagaag tcgccgccag
    32401 gctggaagct ctgatgcgcc gaaccaacgg ccaggccagc aacgagctgc gccacggcaa
    32461 cgtcatgctc gaccccggca aacgtatcgc cacgctggct ggcgaaccct taacgctgaa
    32521 accaaaagaa tttgccctgc tggaattact gatgcgtaac gctggtcggg tactgccgcg
    32581 caaactgatt gaagagaaac tgtatacctg ggacgaagag gtcaccagta atgccgttga
    32641 agtgcatgtg catcatctgc gacgcaaact cggtagtgat tttattcgta ccgtgcatgg
    32701 tattggctac acattaggtg agaaatgaaa tttacccaac gtcttagtct gcgcgtcagg
    32761 ctgacgctaa tctttttaat tctggcctca gtgacctggc tgctttccag ctttgtcgcc
    32821 tggaaacaaa caacggataa cgtcgatgag ttgttcgaca cccaactgat gctgtttgcc
    32881 aagcggttaa gtacgctcga tctcaacgaa atcaacgcgg cagatcgcat ggcacagacg
    32941 ccaaataaat taaaacacgg tcatgttgat gacgatgcgc tgacctttgc catctttacc
    33001 cacgacggca gaatggtcct taatgatggc gataacggag aagatattcc ctatagctat
    33061 caacgggaag gttttgctga cgggcaactg gtgggcgata aagatcaatg gcgttttgtc
    33121 tggatgacct cacctgatgg caaatatcgc atcgttgttg gtcaggaatg ggaataccgt
    33181 gaagacatgg cgctggcgat tgttgccggg caattgatcc cgtggttggt cgcactgccg
    33241 attatgttaa tcatcatgat ggtactactg ggtcgtgaac tcgcgccgct gaacaaactg
    33301 gcgctggcac tacgtatgcg tgaccctgac tcggaaaaac cactaaacgc gactggcgta
    33361 cccagcgaag tgcgtccact ggttgagtcg ttgaatcaac tgttcgcccg cacacatgcg
    33421 atgatggttc gtgaacgacg ctttacctct gacgcagctc acgaacttcg tagcccgtta
    33481 acggcgctga aagtgcaaac cgaagttgcg cagctctctg acgatgatcc gcaggcgcgg
    33541 aaaaaagcac tgctccaatt acattccggg atcgatcgtg ctactcgtct ggttgatcaa
    33601 ctgctcacgc tatcgcggct ggactcactg gataaccttc aggacgtcgc ggagatcccg
    33661 cttgaagatc tcctgcaatc gtcggtgatg gatatttacc acacggcgca gcaggcgaac
    33721 attgacgtgc gactgacact caatgccaac ggcatcaaac gcaccggaca accgctattg
    33781 ctaagtttgt tggtgcgaaa tttgctggat aacgccgtgc gctacagtcc acagggcagc
    33841 gtggtggacg tcacgctgaa tgccgatagt ttcatcgtga gggataacgg ccccggtgta
    33901 acaccagagg cactggcgag aattggcgaa cgcttctatc gcccacccgg acaaaccgct
    33961 accggcagtg gacttgggct atcaattgtc cagcgaatcg ccaagctgca tggcatgaat
    34021 gttgagtttg ggaatgcgga acaaggtgga tttgagacga aggtaagctg gtaagggtaa
    34081 gatttttgct aaattcgcaa aagactttgc acattttgct aatttcaccg taccgctctg
    34141 tgacgtacta tagtcggcaa acgtctcacc ttgaggttaa aaaatgagca acatcctgat
    34201 tatcaacggc gcgaaaaaat tcgcccactc caatggtcaa ctgaacgaca ccctgaccga
    34261 agtggcggat ggcacactgc gcgacctcgg gcatgatgtc cgcatcgttc gcgccgacag
    34321 cgactacgat gtcaaagcgg aagtacaaaa ctttctctgg gctgatgtgg tgttctggca
    34381 gatgccaggc tggtggatgg gcgcgccgtg gacagtgaaa aaatacattg atgatgtatt
    34441 caccgaaggt cacgggactc tgtatgccag cgatggtcgt acccgcaaag atccgtcgaa
    34501 aaaatacggt tctggcggtc tggtacaagg taaaaaatat atgctttccc tgacctggaa
    34561 cgcaccaatg gaagccttca ccgaaaaaga tcagttcttc cacggcgttg gtgttgacgg
    34621 tgtgtacctg ccgtttcata aagcaaacca gttccttggc atggaaccgc tgccgacatt
    34681 tatcgctaat gacgtgataa aaatgcctga tgttccccgc tatactgaag aatatcgcaa
    34741 gcatcttgtg gaaatttttg gttaactaga gctctggctt tagaaggagt taaccatgat
    34801 tacagtaatc gcagaaatcc gtactcgtcc tggtcaacat caccgtcagg cagtgttgga
    34861 tcagtttgct aaaatcgttc caaccgtact gaaagaagaa ggttgccacg gctatgcgcc
    34921 aatggtggat tgcgctgctg gcgtgagttt ccagtctatg gcaccggatt ctatcgtgat
    34981 gattgagcag tgggaaagca tcgcgcatct tgaagcgcat ctgcaaaccc cgcacatgaa
    35041 agcatatagc gaagccgtaa aaggtgacgt gctggagatg aatattcgta tcctgcagcc
    35101 ggggctttga catttctaaa aagaggccgg tgctgctacc ggtctcagcc cagattagcc
    35161 attctttctt cccgttttca gtcctcgatg gaaatcgtta attcgcttaa tggttttaac
    35221 cgcacgctgc ggcactgcgg aagctaccga ccagacaatc atttccagac acaacaatac
    35281 cgtaccatgt agcggcgttt tccctttttc atccccgcgc ggaacatgaa tcacaatact
    35341 ggcgtctttg ctaaaacgtg aatccagcgc gtttgtcagc aaaataacag ggatccccag
    35401 ccgcctcgcc tcacgaagca ttgtcagccc ttcccggtgc gcagattttt gcgccatcat
    35461 gatcagaaca tcgccacgtt gaagcgcgat cagttgctcg gcaaggccaa tacccgtacg
    35521 gtttagtgcc gtggcaggca aacctatgcg gttgaatagt cgtgcggtat agtccgccag
    35581 aatacccgaa gcgccaatgc caaaaattgc gacttgccgt gcctgcacca acaacgctac
    35641 agcttgcgca agcgcgtggc ggttttcagg ctctgacagg acattacagg tatacaaatg
    35701 cccttcaaga acaaagtcga ttgcggtgtt aacatcgctc gtcaagttac tcaccgtcgt
    35761 agacatcttt tcgctggaac taagggctgg accaagccac tgttccagag tgcgtttcag
    35821 atcacgcagc ccggcaaacc ccagggcctg tatagccctg ataactgtgg catcggaagt
    35881 gttcaacgtg gccgcaattt ccatcgccgt ttgctcgagt accacttcac gatgctcatt
    35941 aatgtagctg gcgacaactc ttaacccagg cgttaactga ctcgcgcgag tgcgaaaacg
    36001 ctcaccaaaa atatcaaccc gcactttatc tttctggatc atttagcctc cggtagaggt
    36061 cgccctgcaa tttgtgattg tatctgcgca gccatcagcc ccagagatgc aaaggagtta
    36121 gaaattgcct cttcacgaga aatcagcaca taacgcccgg aacgacaggc agtcagaaac
    36181 tgacaccatc ccggcatgac tttttccatt gctgccagct catcctgagg tttaccaccg
    36241 gtgtcaccgc gccaggttgc aaaaacaaaa tcggcatcca gttcaggtaa acgctccgcg
    36301 ctcacatcca ttcgcccacc ttcaggaatg ctctcaatca gcggcggaaa gcgaaaatca
    36361 gcatcgcgta atacccgccc cagcgaatga tagctatgca tcacattaat ttttccctga
    36421 tttgcctgaa tcaccgatac cgttattttt tggctgtcca gcgtcgcctt cagcgcattg
    36481 atttgtgcct gatagcggcg ttcaagtatc gccagctgcg actgcgttcc tgtaagttct
    36541 gccagcttgc ggtagatttc tggcgcgcct cctttgagat gatcaatgct caccgtgggc
    36601 gcaatttttt ccagttgttc aatgggcgta ttacgggtcg gttctgtgat gatcaggtca
    36661 ggctttgccg ccacaatcgc ttcaatatca atatcagcgg tgccaataaa agcgatcgat
    36721 gaattatcaa agtcgacacc tgtcaataac gcgcctgatc gaatgaaatg gctaccgtca
    36781 ggccgggttc gcccgtggct ggcgacagga ggtacaccga gctcaatcag aggaatagtg
    36841 atatcaagat cgtgtaaaga gacaattctc tttggatgag caggaacagt gactttgcgc
    36901 cctaaatcat cagtaaagac ctgaacaggt tctgtcgcgc gggcaaagaa tgacaacagt
    36961 atcagcagag aaaaaaataa acgcatataa ctcccattaa aaactgttcc tgcgctgcca
    37021 gagcagtagc aaaaagaatg gtccaccaat caatgaaata atgattccgg caggcagttg
    37081 caaaggcaaa aaagccaaac gaccaatgct atccgccaga ataactaaca acgcccccga
    37141 taccgcgctc cccagcagta atgtcgtctg cccgccacgt agaataaaac gtgatatatg
    37201 gggtgctatc aatccgacaa aaccgatgtt accaacacac gaaacgcaga cggctgtcag
    37261 gattattggt gcggcgacac gtaacaacgc cagacgagaa ctattcaccc ctaacccggt
    37321 tgcaacctga tgaccaagca gagcgatatc cgccgtcctg gcggtgaaca gcaataaaag
    37381 agcggctggc aacatccaac aggctgaaat accgactaac atccagttag ctgcatgcag
    37441 gcttcccgat aaccacatca atgcagtttg tacatctcga acatctgcag tggtcataaa
    37501 tacatcgata ccggcggcga aaaaccagga aacaccaata ccaatcagaa caaatcgcgg
    37561 gcgggaaata tcgcgcgcac aaaaaatcac gattaaagcc accaacaggc caccagcaag
    37621 accagccact ggacgccaga acattcccag cattggaaac tggaaaatca gccacagcac
    37681 agccacgcta catccttctt tgacgccgat taacccagga tcggcaaggc cgttacgtgc
    37741 aatggattgc attgcggccc ctgccatacc taacatggcc ccgcatagca atgccatgat
    37801 gactctcggc aagcgaatat cctgcacaat ataacgtgcg tctgtgctta cgttttctgg
    37861 agagaacaat gctcgtccta cttccgatgc cgggacggag aaagagccat gcatcagacc
    37921 gaatatcagt attccaaccg caaacagaaa caacccacca agcaacagca gatttttagg
    37981 gcgtaccagt gtcgaaaact taccaacacg tagtgggcgt agcccgacgt tcattatccg
    38041 actcatttga acatcctcgt cgccataatg acaaaaacag gcgcaccaac taacgccgtc
    38101 atgacaccgg tcgctaactc atagggcgtg aaaagcgtcc gggcaataat atctgccagt
    38161 aataacaaaa gcgcgccgac acaagctgaa agagggagca gaacgcgcag atccgctgat
    38221 accagacgcc ggacaatctg cggtactagc aggccgacaa aaccaatggg gccagcaatc
    38281 gatacggctg cgccacagag caacgcgatc gcaagcaggg tgaatgttcg cgtgcggagt
    38341 acagagacgc ccagcccctg cgccatgcga tcgcccagtg ccaacatgtt aagggagggt
    38401 gcaaggtaaa gggctaacac aaatcccccc agagaaaacc aggcggacgt acccagcgtc
    38461 gcccaatcct gccccgctaa atcacctgct agccatgtcc gcatcgccag cagtgtttgt
    38521 tcgtcaagaa tcaggattgc agccgttatt gaggacacga acgccgatag cgccacgccg
    38581 cataaagtaa ctttcattgg cgttacccct gaacgccctg cggaggaaag cagcaatatc
    38641 aacaggaaca ataacgcgcc ccccgtagat gccagcagtg ggcgtccaac cggaaaggcc
    38701 agtccaagcg cactggcggc aacgacagca agagcggctc cggcatttaa gcctaaaata
    38761 tgtggttccc ccaatgggtt acgaatgaca gcctgcaaca aagcaccagc aacaccaaga
    38821 gaagcaccgg taagcaatgc tgctgctaaa cgcggcaaac ggagtcttac aattacgttg
    38881 tgatcgaagt tttgcggatc aaaatgaaga aatgcctgca acaccgtttg tggtgcaata
    38941 aatcgggcac caatccctgg atataccacg catcccacca gcaataaggc gaggaaaatt
    39001 acagtaacca gccctgtgcg catcatactt tttctacacc acgaaacggc atgaagaacg
    39061 gctttcccgt cagaggattt attgatgcgt gaacatcaac gtcaaagaca gcttttacca
    39121 gttcaggcgt gcaatgctcc ccctcattca acacacgcac cactttcccc tggcgtaaaa
    39181 atatcaacgt atcgccataa ttgacggcaa aattcagatc atgcaacacc acgacgactg
    39241 tacgcccgtg atgacgcgtt aaatcatgta ataactcaag aatttcaacc tgataacgta
    39301 aatcaagcca agttgtgggt tcatccagta ggatataagg cgtcttttgc gccaacacca
    39361 tcgcaatcca gcaacgctgg cgctgcccac cagagagttt ttcaacaggt aaatgtgcaa
    39421 actcctgcgt accggttaat ttcagagcct cttctaccgc ctgctcgtcg gcgtcgctcc
    39481 attgacgaat gaaattctgc cagggaaaac gcccacgcga caccagttca taaacggtta
    39541 agccctccgg caataatggc gactgcggca agatcccaag tctacgggat aacgctttcg
    39601 ttggttgctc gtgaatcgct ttgccatcaa gcaatatcga accgcccata ggttgcaaga
    39661 tacgtgcaat ggtgctgagc aacgtcgact ttccacagcc gtttgcccca accagaacgg
    39721 tcattttttc tgtgggaacg gagaaagaaa caccatcaac aatgattttc ttgtgatacc
    39781 cggcagaaac attgtcgagg atcagcccct gcccttttgc gttatgtgcc acgtgtacgc
    39841 caacttaatc aaagacaaac aaaaataaaa ctcattctcc tttgagagtt tgacgccaca
    39901 aaatgtagta gtcaagcagt taaaagccac
//
LOCUS       DAPHCV010000034        39929 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.034, whole genome shotgun sequence.
ACCESSION   DAPHCV010000034 DAPHCV010000000
VERSION     DAPHCV010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.034"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(77..1201)
                     /locus_tag="RH033_001793"
     CDS             complement(77..1201)
                     /locus_tag="RH033_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001765453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HDV2990989.1"
                     /translation="MSTLFTINACKTFGCRNLGLASSEDYSWPDYKLGYPALHCRACG
                     SYPPLFDEQQFRDWLSVHLSTWASEKGHFCPVCYGTDMICYGHNPQGSQRIQCRNCKK
                     VWTPKQYQKEITHPQAIETVQLFIPFQGASAVQKLYVLVSLDATRGNILHLSTNYTQH
                     QTGDSLRYSYKGNTEPTMHHRDIVQKVDMREAQFLRRSQFDEIQYGSAVLKRNGKGAI
                     LRPVITAHGHFRILKIRYPDVKTHIISHECFLRGAIITAWADQFRQQQGELWFVEEEI
                     SDSNADTPWHFKGTTYHGWWQNQWQRWEQGNNCKMVCLLTGASLERGANVSLATSRCF
                     ITWLTDQHDFTQSALLSAGRVTQMLTSLALKYNESLTPSC"
     gene            1334..2887
                     /locus_tag="RH033_001794"
     CDS             1334..2887
                     /locus_tag="RH033_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDV2990990.1"
                     /translation="MKIKILAAGIALTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIA
                     DGKRDIGGWANITTLVKQEKAKNKATWFFDAGDYFTGPYISSLTKGKAIIDIMNTMPF
                     DAVTIGNHEFDHGWDNTLLQLSQAKFPIVQGNVFYQNSSKSFWDKPYTIIEKDGVKIG
                     VIGLHGVFAFNDTVSAATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPARQS
                     SMGGTDVRRALDKDIQTASQVKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVG
                     KLVLDYKEKPHNFTVKNFELKTIYADEWKPDPQTKQVIDGWNKKLDEVVQQTVAQSPV
                     ELKRAYGESASLGNLAADALLVAAGKNTQLALTNSGGIRNEIPAGAITMGGVISTFPF
                     PNELVTMELTGKQLRSLMEHGASLSNGVLQVSKGLEMKYDSSKPVGQRVITLTLNGKP
                     IEDATVYHIATQSFLADGGDGFTAFTEGKARNTTGGYYVYHAVVDYFKAGNTITDEQL
                     NGMRVKDIK"
     gene            3269..4159
                     /gene="metF"
                     /locus_tag="RH033_001795"
     CDS             3269..4159
                     /gene="metF"
                     /locus_tag="RH033_001795"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDV2990991.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPAELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLRRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            4488..6668
                     /gene="katG"
                     /locus_tag="RH033_001796"
     CDS             4488..6668
                     /gene="katG"
                     /locus_tag="RH033_001796"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HDV2990992.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGGGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            6762..7667
                     /gene="yijE"
                     /locus_tag="RH033_001797"
     CDS             6762..7667
                     /gene="yijE"
                     /locus_tag="RH033_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HDV2990993.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(7694..8311)
                     /locus_tag="RH033_001798"
     CDS             complement(7694..8311)
                     /locus_tag="RH033_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HDV2990994.1"
                     /translation="MKASLALFSLLTVFTSYSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVLQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     AYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPTSKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFAK"
     gene            complement(8587..9690)
                     /gene="gldA"
                     /locus_tag="RH033_001799"
     CDS             complement(8587..9690)
                     /gene="gldA"
                     /locus_tag="RH033_001799"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HDV2990995.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDDGEFDRYLMLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(9701..10363)
                     /gene="fsa"
                     /locus_tag="RH033_001800"
     CDS             complement(9701..10363)
                     /gene="fsa"
                     /locus_tag="RH033_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDV2990996.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVKEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(10375..12876)
                     /gene="ptsP"
                     /locus_tag="RH033_001801"
     CDS             complement(10375..12876)
                     /gene="ptsP"
                     /locus_tag="RH033_001801"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDV2990997.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPISLTNLNPQIIRARIVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIPLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLAAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            13185..14264
                     /locus_tag="RH033_001802"
     CDS             13185..14264
                     /locus_tag="RH033_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HDV2990998.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGVMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICVPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCSAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTEEKEDDLDLDFEIN"
     gene            14279..14599
                     /locus_tag="RH033_001803"
     CDS             14279..14599
                     /locus_tag="RH033_001803"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDV2990999.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            14650..16947
                     /locus_tag="RH033_001804"
     CDS             14650..16947
                     /locus_tag="RH033_001804"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDV2991000.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVIMRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCT
                     DGQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQDEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDAHDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMIFDQQRLSFDELLSVLKANFATPEGE
                     KVRTRLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            16913..17791
                     /locus_tag="RH033_001805"
     CDS             16913..17791
                     /locus_tag="RH033_001805"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HDV2991001.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDAAQARDVVKMNLQRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKEVAAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            17793..18134
                     /locus_tag="RH033_001806"
     CDS             17793..18134
                     /locus_tag="RH033_001806"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDV2991002.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(18121..18972)
                     /locus_tag="RH033_001807"
     CDS             complement(18121..18972)
                     /locus_tag="RH033_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV2991003.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFIDTGAGAALVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQSQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(19197..20930)
                     /locus_tag="RH033_001808"
     CDS             complement(19197..20930)
                     /locus_tag="RH033_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HDV2991004.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAT
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(21113..23764)
                     /gene="ppc"
                     /locus_tag="RH033_001809"
     CDS             complement(21113..23764)
                     /gene="ppc"
                     /locus_tag="RH033_001809"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HDV2991005.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GHEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(24066..25217)
                     /gene="argE"
                     /locus_tag="RH033_001810"
     CDS             complement(24066..25217)
                     /gene="argE"
                     /locus_tag="RH033_001810"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HDV2991006.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            25371..26375
                     /gene="argC"
                     /locus_tag="RH033_001811"
     CDS             25371..26375
                     /gene="argC"
                     /locus_tag="RH033_001811"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDV2991007.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDVAFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVSQVQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            26383..27159
                     /gene="argB"
                     /locus_tag="RH033_001812"
     CDS             26383..27159
                     /gene="argB"
                     /locus_tag="RH033_001812"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDV2991008.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            27220..28593
                     /gene="argH"
                     /locus_tag="RH033_001813"
     CDS             27220..28593
                     /gene="argH"
                     /locus_tag="RH033_001813"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDV2991009.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKALEDLPLSELQKFSQVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            29091..30305
                     /locus_tag="RH033_001814"
     CDS             29091..30305
                     /locus_tag="RH033_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HDV2991010.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTASGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFDEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            30372..31655
                     /locus_tag="RH033_001815"
     CDS             30372..31655
                     /locus_tag="RH033_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2991011.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTEEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            31907..32824
                     /gene="oxyR"
                     /locus_tag="RH033_001816"
     CDS             31907..32824
                     /gene="oxyR"
                     /locus_tag="RH033_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HDV2991012.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(32807..34207)
                     /gene="sthA"
                     /locus_tag="RH033_001817"
     CDS             complement(32807..34207)
                     /gene="sthA"
                     /locus_tag="RH033_001817"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HDV2991013.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            34539..35189
                     /gene="fabR"
                     /locus_tag="RH033_001818"
     CDS             34539..35189
                     /gene="fabR"
                     /locus_tag="RH033_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HDV2991014.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            35189..35548
                     /locus_tag="RH033_001819"
     CDS             35189..35548
                     /locus_tag="RH033_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HDV2991015.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLL
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(35588..36688)
                     /gene="trmA"
                     /locus_tag="RH033_001820"
     CDS             complement(35588..36688)
                     /gene="trmA"
                     /locus_tag="RH033_001820"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HDV2991016.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPILR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQQAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      36802..36992
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            37057..38901
                     /gene="btuB"
                     /locus_tag="RH033_001821"
     CDS             37057..38901
                     /gene="btuB"
                     /locus_tag="RH033_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HDV2991017.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFQEPLSAVLA
                     PVTVVTREDIERWQVSSVNDVLRRLPGVAIAQNGGEGQLSTIFIRGTNSNHALVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAIYGSDAIGGVINIITSRENTGTEI
                     SAGWGSNSYQNYDISTQQQIGENTQVTLLGDYAYTRGFDVVAYGNTGMQPQSDRDGFL
                     SKTLYGKLEHNFSDTWSGFVRGYGYDNRTKYDAWYSPGSPLIDTRKLYSQSWDAGLRY
                     AGETLQSQLVSSYSHSKDYNYDPHYGRYDASANLDEMKQYNLQWTNSVTVGHGDVGAG
                     IDWQKQSTTPGTGYLPKGYDQRNTGIYLTGLQKVGDFTLEGAVRNDDNSQFDRHTTWQ
                     SSVGWEFIEGYRFIASYGTAFKAPNLGQLYGYKGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDISDMINCTQNCERYYNDAKVHIKGIESTANFETGPLVHTVSYDYTDARN
                     ALTDKPLERRPKQQVKYQLDWQVFDFDWGVTYQYLGSRYDSDYSSWPYKSVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            38846..39703
                     /gene="murI"
                     /locus_tag="RH033_001822"
     CDS             38846..39703
                     /gene="murI"
                     /locus_tag="RH033_001822"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDV2991018.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLD"
BASE COUNT         9310 a         9796 c        10825 g         9998 t
ORIGIN      
        1 gtcatacatt tatatattta attaaatgaa aggtagtgat ctctcttaat ttatggtcgt
       61 ttgtcgagtg tattgactaa caagatggag tgagcgattc attgtatttt aacgccagtg
      121 aggtcagcat ttgcgttacg cgacctgcgg ataataacgc gctttgggta aagtcgtgtt
      181 ggtctgtcag ccatgtgata aaacaacgac tggttgccag agaaacattt gcgccccttt
      241 ctaagctggc tcccgtgagt aggcagacca tcttgcagtt attcccctgc tcccagcgct
      301 gccactgatt ttgccaccaa ccatggtatg tcgttccctt aaaatgccag ggcgtatcag
      361 cgttactgtc actgatttct tcttctacga accaaagttc accttgttgt tggcggaact
      421 gatctgccca ggctgtaata attgcgcctc ttagaaaaca ttcgtgtgaa ataatatgcg
      481 ttttgacatc tggatagcgg attttcagta ttctgaaatg cccgtgtgcc gtgataaccg
      541 ggcgtagtat ggctccctta ccgttgcgct tgagtacagc actgccatac tgaatttcat
      601 cgaactggct ccggcgcaag aattgcgctt cacgcatatc taccttctgc acgatatcgc
      661 gatggtgcat agttggttct gtgttgccct tgtaactgta ccgcagactg tctcctgtct
      721 gatgttgcgt ataattcgtg gagagatgaa gaatgttgcc acgagtggcg tcaagactga
      781 ctaaaacata cagtttttgt actgcgctgg ccccttgaaa gggtatgaac agttgaacgg
      841 tttcaatggc ctggggatgc gtaatttctt tctgatactg tttaggcgtc cagacttttt
      901 tgcagttacg gcattgaatg cgttgcgaac cctgtggatt gtgaccataa caaatcatgt
      961 ctgtgccata acataccggg cagaaatgac ccttctccga ggcccacgta gataagtgaa
     1021 ctgacaacca gtcacgaaat tgttgctcgt caaacaacgg cgggtaactg ccacatgccc
     1081 gacagtgcag tgccggataa cccagtttat agtctggcca actgtaatct tccgatgagg
     1141 ccaatcccaa atttcggcaa ccgaacgttt tacaggcatt aatcgtaaac agagtcgaca
     1201 tatatcacca acgatttcac aaggtcgttg attttgtccg gcaactatcc cctgcagatg
     1261 tgattactct cacataaata agtctttcag aacgtaatgt agcaatcctt tatctcactg
     1321 aaagggattg tttatgaaaa ttaaaatact tgctgcgggt atcgcattaa cactgccgtt
     1381 ctgggcttgc gctaaagatg tcactattat ttataccaac gacctccatg cgcatgtaga
     1441 accttataaa gtgccgtgga tcgctgacgg taaacgggat attggcggct gggcaaatat
     1501 cactacgctg gttaagcaag aaaaagcaaa aaataaagcg acatggtttt ttgatgcggg
     1561 cgactatttt accgggccat atatcagcag cctgaccaaa ggtaaggcta ttatcgatat
     1621 tatgaatact atgccgtttg atgccgtcac gataggcaac catgaatttg accacggctg
     1681 ggacaacaca ttattacagt tgagtcaggc aaaattcccg attgtgcagg gcaatgtttt
     1741 ttatcagaac agcagtaaat cattctggga taagccctat accatcatcg aaaaagacgg
     1801 tgtgaaaata ggcgttatag gtttgcacgg tgtatttgcc tttaatgaca cagtatctgc
     1861 tgccacgcga gtgggtattg aggcgcgtga tgaaattaaa tggctacaac gttatatcga
     1921 tgaacttaaa ggcaaggttg atttgaccgt cgccctgatc catgaaggtg ttccggcccg
     1981 ccagtccagt atgggaggca cagatgtgcg tcgcgcgctg gataaagata ttcagacggc
     2041 aagtcaggtg aaagggctgg atattttgat caccggacat gcacatgtgg gtacgccgga
     2101 accgattaaa gtcggcaata cgctcatcct ctccactgac agcggcggga ttgatgtggg
     2161 caaactggtt ctcgactaca aagagaagcc gcataatttt acggtgaaaa acttcgagct
     2221 taaaaccatt tacgctgacg agtggaagcc cgatccacaa acgaaacagg tgatcgacgg
     2281 ctggaataaa aagcttgatg aagtggtgca acaaacggtg gcgcaatcgc cggttgaatt
     2341 aaaacgtgcc tatggtgaat cggcttccct cgggaacctg gcggctgacg ctttactggt
     2401 agcggcgggt aaaaacaccc aattggcgtt aaccaactct ggcggcattc gcaatgagat
     2461 ccccgcaggg gcgatcacga tgggcggcgt catcagtacg ttcccgttcc ccaacgaact
     2521 ggtgacgatg gaactcacgg gtaaacaatt acgcagtctg atggaacacg gcgcaagttt
     2581 aagcaatggc gttttacagg tatcgaaagg cctggaaatg aagtacgaca gcagcaagcc
     2641 ggttggtcag cgggtaatca cactgactct gaatggcaaa cccattgaag atgcgacggt
     2701 ttatcacatt gctactcaga gtttccttgc tgatggtgga gatggtttta ccgcctttac
     2761 cgaagggaaa gcgcgtaaca caacgggcgg ttattacgtt tatcacgcgg tggttgatta
     2821 cttcaaagcg ggcaacacca ttacggatga acagctcaac ggtatgcgcg tgaaggatat
     2881 caaataaaag tgcgcactga attgactcaa ctgtttgtta cccgttgatt gcgcagatcc
     2941 tgtattcggc ctacgcgagg gcgcaggccg aatcagatgc cttagcatca ccatccggtg
     3001 gcaggacgtt gtttttcgaa ttagcccagg tgtcgctgtg ttgtcagaat atgagtcaaa
     3061 acgtgcgagg tggattgtgc tatagagaaa tcttttatcg cctctaatta agatgagttt
     3121 ttttcatctt ccctgatttt tcctcaccat cattggttat ttttcggttg acgcccttcg
     3181 gcttttcctt catctttaca tctggacgtc taaacggata gatgtgcaca acacaacata
     3241 taacgataag cgattgatga ggtaaggtat gagctttttt cacgccagcc agcgggatgc
     3301 cctgaatcag agtctggcag aagtccaggg gcagattaac gtttcgttcg aatttttccc
     3361 gccgcgtacc agtgaaatgg agcagaccct gtggaactcc atcgatcgcc ttagcagcct
     3421 gaaaccgaag tttgtatcgg tgacctatgg cgcaaactcc ggcgagcgcg accgtacgca
     3481 cagcattatt aaaggcatta aagatcgcac cggcctggaa gcggcaccgc atcttacctg
     3541 tattgatgcg acgcctgccg agctgcggac cattgcacgc gactactgga ataacggtat
     3601 tcgccatatc gtggcgctgc gtggcgacct gccgccggga agtggtaagc cagaaatgta
     3661 tgcttctgac ctggtgacac tgttaaaaga agtggcagat ttcgatatct ccgtggcggc
     3721 gtatccggaa gtacatccag aagcgaaaag tgcccaggcc gatttactca acctgagacg
     3781 aaaagtggat gccggagcca accgcgccat tactcagttc ttcttcgatg tcgaaagcta
     3841 cctgcgtttt cgcgatcgct gcgtctcggc aggcattgat gtggaaatca ttccgggaat
     3901 tttgccggtt tcgaacttta aacaggcgaa gaaatttgcc gacatgacca acgtgcgtat
     3961 tccggcgtgg atggcacaaa tgttcgacgg cctggatgac gatgctgaaa cccgcaagct
     4021 ggtgggcgcg aatattgcca tggatatggt gaagatttta agccgtgaag gagtgaaaga
     4081 tttccacttc tatacgctta accgcgctga aatgagttac gcgatttgcc atacactggg
     4141 tgttcgacct ggtttataaa tagtgtgcct tttgtgaaaa tcacacagtg atcacaaatt
     4201 ttaaacagag cacaaaatgc tgcctcgaaa tgagggcggg aaaataaggt tatcagcctt
     4261 gttttctccc tcattacttg aaggatacaa atctaaaacc cttttttata aagcatttat
     4321 cctaaatcgg acataatcaa aaagtcttaa ttacgatcaa tttgatctac atctctttaa
     4381 ccaacaatat gtaagatctc aactatcgca tccgtggatt aattcaatta taacttctct
     4441 ctaacgctgt gtatcgtaac ggtaacactg tagaggggag cacattgatg agcacgtcag
     4501 acgatatcca taacacgaca gccaccggca aatgcccgtt ccatcagggc ggtcacgacc
     4561 agagcgcagg tgggggcaca accacgcgcg actggtggcc aaatcaactc cgtgttgacc
     4621 tgttaaacca acattctaat cgttctaacc cgctgggtga ggactttgac taccgcaaag
     4681 aattcagcaa attagattac tacggcctga aaaaagatct gaaagccctg ctgacagaat
     4741 ctcaaccgtg gtggccagcc gactggggca gctacgccgg tctgtttatt cgtatggcct
     4801 ggcacggtgc gggtacttac cgttcaatcg atgggcgagg cggcgcgggt cgtggtcagc
     4861 aacgttttgc accgctgaac tcctggccgg ataacgtaag cctcgataaa gcgcgccgcc
     4921 tgttgtggcc aatcaaacag aaatatggtc agaaaatctc ctgggccgac ctgtttatcc
     4981 tcgcgggtaa cgtggcgctg gaaaactccg gcttccgtac cttcggtttt ggtgccggtc
     5041 gtgaagacgt ctgggaaccg gatctggatg ttaactgggg tgatgaaaaa gcctggctga
     5101 ctcaccgtca tccggaagcg ctggcgaaag caccgctggg tgcaaccgag atgggcctga
     5161 tttacgttaa cccggaaggc cctgatcaca gcggcgaacc actttcggca gcagcagcta
     5221 tccgcgcgac cttcggcaac atgggcatga atgacgaaga aaccgtggcg ctgattgcgg
     5281 gtggtcatac gctgggtaaa acccacggtg ccggtccgac atcaaacgta ggtcctgatc
     5341 cagaagcagc accgattgaa gaacaaggtt taggctgggc gagcacttac ggcagcggcg
     5401 ttggcgcaga tgccattacc tctggtctgg aagtggtctg gacccagacg ccgacccagt
     5461 ggagcaacta tttcttcgag aacctgttca agtatgagtg ggtacagacc cgcagcccgg
     5521 ctggcgcaat ccagtttgaa gcagtagacg caccggaaat tatcccggat ccgtttgatc
     5581 cgtcgaagaa acgtaaaccg acgatgctgg tgaccgacct gacgctgcgt tttgatcctg
     5641 agttcgagaa gatctctcgt cgtttcctca acgatccgca ggcgttcaac gaagcctttg
     5701 ctcgtgcctg gttcaaactg acgcacaggg atatggggcc gaaatctcgt tacatcgggc
     5761 cggaagtacc gaaagaagat ctgatctggc aagatccgct gccgcagccg atctacaacc
     5821 cgaccgagca ggacattatc gatctgaaat tcgcgattgc ggattctggt ctgtctgtta
     5881 gtgagctggt atcggtggcc tgggcgtctg cttctacctt ccgtggtggc gacaaacgtg
     5941 gtggtgccaa cggtgcgcgt ctggcattaa tgccgcaacg tgactgggat gtgaacgccg
     6001 cagccgttcg tgctctgcca gttctggaga aaattcagaa agagtctggc aaagcttcgc
     6061 tggcggatat catcgtgctg gcaggtgtgg ttggcgttga gaaagccgca agcgccgcag
     6121 gtttgagcat tcatgtaccg tttgcgccgg gccgcgttga tgcgcgtcag gatcagactg
     6181 acattgagat gttcgaactg ctggagccaa ttgctgacgg tttccgtaac tatcgcgctc
     6241 gtctggacgt ttccaccacc gagtcactgt tgattgataa agcacagcaa ctgacgctga
     6301 ccgcgccgga aatgactgcg ctggtgggcg ggatgcgcgt actgggggcc aacttcgatg
     6361 gcagcaaaaa cggcgtcttc actgaccgcg ttggcgtatt gagcaatgac ttcttcgtga
     6421 acttgctgga tatgcgttac gagtggaaag cgaccgacga atcgaaagag ctgttcgaag
     6481 gccgtgaccg cgaaactggc gaagtgaaat acaccgccag ccgtgcggat ctggtctttg
     6541 gttctaactc tgtcctgcgt gcggtggcgg aagtctacgc cagcagcgat gcccacgaga
     6601 agtttgttaa agacttcgtg gcggcatggg tgaaagtgat gaacctcgac cgtttcgacc
     6661 tgctgtaatc tgaccttctt cagcgactgc ctttcaggca gtcgctgaag tttctttacc
     6721 ggcgtatagt gtccacagga aaactacaca ctggatctct catgtctgcc gcaggaaaga
     6781 gcaacccact ggcaatcagt ggcctggttg tgctcacact tatctggagt tatagctgga
     6841 ttttcatgaa gcaagtcacc agctacatcg gtgccttcga ctttaccgcc ttacgctgca
     6901 ttttcggcgc tctcgtttta ttcatcgtcc ttttattacg tggtcgcgga atgcgcccga
     6961 caccgtttaa atacacctta gccattgccc tgttacaaac ctgcgggatg gtcggcctgg
     7021 cgcagtgggc gctggtcagc ggaggagcgg ggaaggtggc aatcctgagc tataccatgc
     7081 cgttctgggt ggtaattttc gccgcgttgt ttctcggtga acgcctgcga cgcggacaat
     7141 atttcgcgat tctgattgcc gctttcggct tatttttggt gttgcagccg tggcaactcg
     7201 atttctcttc gatgaaaagc gccatgctgg caatcctctc cggtgtgagt tggggggcga
     7261 gcgcgattgt tgctaaacgt ctgtatgccc gccatccgcg cgtggattta ttgtcgctaa
     7321 catcctggca gatgctgtat gcggcgctgg tgatgagcgt ggtcgcttta ctggtgccgc
     7381 aacgtgaaat tgactggcaa cccaccgtgt tctgggcgct ggcctacagt gcgattctgg
     7441 cgacagcgct ggcgtggagc ttatggttgt ttgtattgaa aaacttgcct gccagtattg
     7501 ccagcttaag cacactggcc gttcccgttt gcggcgtact cttttcctgg tggctgctcg
     7561 gtgagaatcc gggggcagtt gaaggtagcg gtattgtgct gattgtactg gcactggcgc
     7621 tggtgagccg taagaaaaaa gaagccgtca gtgtaaaaag gatctgaatt ttttcttcat
     7681 gtggggagat ctcttattta gcaaaatacc gatagtgccc caccatccgc cagctaaaca
     7741 gtacatcttc ttcctgcgcg cctgcgccaa tgttatgtat caccagcggc gtaccgtcgc
     7801 gggcgaagcc atctgacacc accccaatat gtgccagccc gttatctaat cgccaggaga
     7861 caatatcgcc cgcttgatag tcactggggt tcttgctggt ggggcgtgtt ttatcgtggc
     7921 gggtaaacca ggtttccaga ttaggcaccc ggcggtgatc gatgttgctg tccgggcgct
     7981 ttaactgcca tttttgcggg tacgcagcaa aattcttcgc catatcttcg tgcaccagtt
     8041 tctgcaaatc gaccttctgg ctgcgcaatg cacggatcac cacatcggaa catacgccgc
     8101 gttcttgcag gacatcaccg ccaggatagg taagctgcac atacgccgga tcgtaaaata
     8161 gcgtgctgcc gatttgctgt ctggcgccgt ctgcgatggc aagattggtg ttggcctgga
     8221 tttgtaccac ggttggtgga acggcgggag attttaagga gtaactggta aataccgtca
     8281 gaagactgaa taacgccagt gaagctttca tctgtttcgt ccgtgaaaat aagagtcaca
     8341 gattcgacct tccgggctaa cgattgttct gtggagaaaa tgtccagaag cggataaaaa
     8401 aacacccgaa caaggtggaa gtttcagatt actggcacag cgtgccggat gcggcgtgaa
     8461 cgccttatcc ggcctacaaa agtacgtaaa tttactatat tgcagagatt gcgtaggcct
     8521 gataagcgta gcgcatcagg caattttgcg ttcaaactcc cggacaagcc gggagtttgg
     8581 agtaggttat tcccactctt gcaggaaacg ctgaccatac tggtcggcta ccagcagtgc
     8641 ggcgtaaacc tgatctggcg tcgcgccgcc aggcatgttg tggatggttt caccttctgc
     8701 acatgccgct tctgccacaa ttcgcatttt cgccgggaca tcttctttaa tatccagttg
     8761 agcgagagtt attggcaaac ctaccgcatg gctaagtgca gctacggttt cgatttcctc
     8821 caccggcgcg ttttccagaa ccagctgcgt cagcgtacca aatgccactt tttcaccgtg
     8881 gtaatagtga tgcgcgtccg ggatagcggt caggccgtta tgcaccgcgt gagccgccgc
     8941 cagcccgccg ctttcaaaac caacgccgct caaataggtg ttcgcttcaa tcacgcgctc
     9001 cagcgccggg gttactacat gctgctcggc ggcaagcatc gctttttcgc cttcttccag
     9061 cagggtgttg tagcacagtt cagccagtgc cagcgccgcc tgggtgcact tgccgcccgc
     9121 catggtggtc gcgccgctac gagagcaagc acgcgcttca aaccaggttg ccagcgcatc
     9181 gccgataccc gccgctaaca gacgtgcagg tgcgccagcg acgattttgg tgtcgacaat
     9241 gaccatattc gggttatttg gcaacatcag atagcggtca aattcaccat catcggtgta
     9301 gataacagac aatgcgctgc acggtgcgtc ggtagaggcg atagtcggtg cgatcgctac
     9361 cggaacaccc atgaaatgtg ccagtgcttt ggcagtatcg agagtttttc cgccaccgat
     9421 accgagaatt gcgccacact gcgcagtctc cgcgatgcca cgcagacggt cgatctcatt
     9481 ttgcgaacat tcaccgccaa acggcgcaat ttctactacc agtccagcat ctttaaagct
     9541 tttctcgaca gtggattgag caaaacctaa aacaaatttg tcacccacca ctaaccagcg
     9601 ttctgccagc ggcttcaggt attcgcccag acgattaatc acatcagcgc cctggatgta
     9661 tttacccggt gattgaataa tgcggtccat aattgctcct ttagagatga gtagtgccaa
     9721 atgcggcatt ccagtcgtgt tcgaacttct ctatagctga ctctaccgca ggggtgttga
     9781 gcatttgttg cgctacatct aagggcaggg tgatggattc acatcctgcc agtaaacagt
     9841 ccagcgcctg acgtggtgtt ttaaagctgg cagccagcac catgctttct ggcgcatgca
     9901 tttccaacag cgcttgcagc tcctgtacag tacgaatgcc gtcaccgccc tgggcatcta
     9961 cgcggttaac gtatggagca acgtatttgg ctccagccag cgccgccagt aatccttgcg
    10021 ctgcgctgta cactgcggtt cccagcgtag taatgccttc tttcttcagc attttaattg
    10081 ctgccagacc ttcagaggtt accggaattt taaccacaat gcccggaata gcgtcgcgca
    10141 ggcgtttcgc ttctttcacc ataccctgcg cgtcgcggct catggtctga gcaaacagaa
    10201 ttccctcatc accgatcgct ttttgaaggc gcggcagcac ttcccagatg gactccttgc
    10261 tggcagcgat aatgctcggg ttagttgtca ccccggcaat cgggaatatg cgtgccagac
    10321 gttcgacttc tgcgacgtta gcggtgtcca gatacagttc catgatgttt ccttttacag
    10381 ttccagttca tgttgcagca ggctggcgat agcgtcggca gaggcggcgt taaccagcgc
    10441 gttacggaat tcttcgtgca tgatgcgacg agcgaggcgc gagaaaatgc gcatgtgctg
    10501 atcgcccgca gcatgtttgt tcagggttaa cataatgatg aattgcgctt catcatcgcc
    10561 ccagcgcacc ggcgctgcca gacgcgccac gctgatggtg gattgctcaa tgtgttctga
    10621 tttgctgtgg ggaatggcaa aactaaagcc cagaccggta gagaaaacgg cctcgcgcgc
    10681 ccacaagtcg gcttccagtt tacgcggata gcgacagcgg cccgccagca gcaggttatc
    10741 ggtcatgcct ttgagcactt cttctttgct gcgccagtcg ctttccagtg tgatgcactc
    10801 ggcggtgacc agcggtgcgt cttgttgggt catgcggaat tgcgccagca ggtgttccac
    10861 ttccagcgaa gtacggcagg ccattgcctg gttgagcaac ttgcggcact cacggctatc
    10921 aagttgcgcc atccgcgctt tcgccgccgg aattgatggt gcgctcatgc tgagttcatc
    10981 cagccctaaa ccgaccagca acggcagcac ggaacctttc gctcccagtt caccgcacag
    11041 accaatccat ttgccctggc gatgcaccgc ctgcacggcg taatcgagcg cccgcaagaa
    11101 tgctggattc aggctgttgt agtgacgagt caccttagcg ttatcgcgat ccactgccag
    11161 tagatattgc gtcaggtcat tactaccaat actaaagaaa tcaatctctt cgcagcattg
    11221 atcgatgatg aacatcaccg acggcacttc cagcatgatg ccgagtggaa tcttctcatc
    11281 aaacggaatg tgttcgttac gcagttgctg tttggcttcc gccagttttt ctttcaccca
    11341 taagatctct tccattgagg agatcatcgg gatcatgatt ttcaggctgc cgtgagcaga
    11401 ggcgcgcagg atcgaccgta gttgtgtggt aaacaacgac gcgtattctt cataaatacg
    11461 caccgcgcga taaccgagga acgggtttgc ctctgcggga atattcagat aatcaacggg
    11521 tttgtcaccg ccaatatcca tagtgcgcac aataatgctg cgtccgttgg cggactccag
    11581 cgcctgacaa aaaatgttgt acagctcgct ttcgcccggt gcactggtgc gatccatata
    11641 gagcatttca gtgcggaata aaccaacgcc ttccgcacca ttgccgaatg cggcctgcgc
    11701 ttccacggag tgagcgatgt tagcggcaat ttcaatgcgg ataccgtcag cggtacgggc
    11761 ttgttgggtc agccagacac gctgttgctc acgcagggcg tcctgtacgc gggcttcttg
    11821 ctgataataa cgggccacgg cttcccctgg ctcaaccaca atcgcccctg cgttgccgtc
    11881 gatataaatc gtttgatgct gccacggagt aagggcatca atatccacac ctaccagcgt
    11941 cggaatgttg aacgaacggg caaggatcac cgtatgtgag gtggtgccgc cacttttcag
    12001 taatagacct ttgaggtgat ttttatccag ttcgaggaac tggctggggg tcagttcatc
    12061 agccatacaa atggcgggct gcgtcagttt gcccggtgcc gggaagcgtt gctcaccgta
    12121 gatttgctgg agtaactgga agcagacgtc gcgtacgtcc agggcacgtt cttgcaggta
    12181 gctgctgctg gaacgggcga actcttcgca aaagtgattc gcactggcaa caatcgcttc
    12241 ggcgcagctt aatccggcgc tgacacctgc cagtaaatgt tcgcgcaggg aagtatcgcc
    12301 agccagggat cggtgcgctt ccagaatcgc gctggtagca ccgtcgctat ccagcagacg
    12361 aaactctatg tttttcagta ccagcgtcag accgttttcc agtgcggatt gttcggcgtc
    12421 aacgcctttg gctgcgggaa gattacccag cgcattgaga tctaaagaag agatcggcgt
    12481 cagaatgccg cctgcgctac cgctgcacac gatgcgagca cggataattt gcggattcag
    12541 attggtcagt gaaatcggca gtggttccag ttcgtcagat tttacttccg ccagcggtgc
    12601 gtcgcagtgg gggaattcat cgcgcagcca ctggcttaaa cgctggtgcg cttcctgttc
    12661 gtcggccccg gaaatcagta actggcagtt atcgcccacc agcgtatcgg tgccaatcag
    12721 cgcaagggcg cttttggcgt tgcccttgcg gtcagtgcgc aggttatgcc actcaatttg
    12781 tgatgaaaaa gtattacaca gcgtttcaac gtggcttgcc ggacgcgcat ggacgccgtt
    12841 aggtagctca caaataaatt ccacaatcag ggccattgcc tttctcccgg tacggttttc
    12901 tgtttacagg ataagagcgc acggtaacgg cctgccatgt gacaaatctg ccaaaagctg
    12961 gacaaatgta atgtaaccgt caatttgcga cgcgtctcac aagacgccgt tttgcggcat
    13021 gcttccggat tatcgcaagt tatgaggcgg atcgcatttt tgtactgata ttacaaaagt
    13081 ccagtaaatg gcctttttat ccactgtttg ctccgcctgc gattgcctat tgtttcttcc
    13141 atcaaaaata tggacatggg ctttcgcaag cccgggagca gcttatgaat gagttggtgc
    13201 agatcctgaa aaatacccgt cagcatttaa tgacgggcgt ttcacacatg attcccttcg
    13261 tggtatcggg cggtattttg ctggcggttt ccgtcatgtt gtatggcaaa ggcgcagtgc
    13321 cggatgccgt agccgatccg aatctgaaaa aactgtttga tatcggcgtt gcaggtttga
    13381 cgctgatggt gcctttcctc gccgcttaca tcggttattc cattgcagaa cgttctgcgc
    13441 tggctccatg cgctatcggt gcctgggttg gtaacagctt tggcgcgggc ttctttggtg
    13501 cgctgatcgc cgggattatc ggcggcatcg tggtgcatta cctgaagaaa attccggtgc
    13561 ataaagttct gcgctcggtg atgccaatct tcatcattcc aatcgtcggc acactgatta
    13621 ccgctggcgt catgatgtgg gggctgggcg agccggtggg ggcgttgacc aacagcctga
    13681 cccagtggct tcaggggatg cagcagggca gcattgttat gctggcggtg atcatgggtc
    13741 tgatgctggc gttcgatatg ggcggcccgg ttaacaaagt ggcctatgcc ttcatgctga
    13801 tttgcgttgc tcagggtgtt tataccgtgg tggctattgc cgctgttggg atttgtgttc
    13861 caccgctggg gatggggctg gcgacgctga ttggtcgtaa aaatttctcc gcagaagagc
    13921 gcgaaaccgg caaggcggca ctggtgatgg gctgtgttgg cgttactgaa ggggcgattc
    13981 ctttcgccgc tgccgatccg ctgcgtgtca ttccttccat catggtcggt tcagtttgta
    14041 gtgcggtaac tgcggcgctg gtcggtgcgc agtgctatgc aggctggggt ggtctgattg
    14101 tgctgccggt ggttgaaggt aagttgggtt acatcgcggc agtggctgtc ggggcggttg
    14161 tgacggcagt ctgcgtgaac gtgctgaaaa gtctggcgcg taaaaatggg tcttcaactg
    14221 aagagaagga agacgacctg gatctggatt ttgaaattaa ttaattgagg aacgagccat
    14281 gacgaaaatt atcgcagtaa ccgcatgtcc ttcaggtgtt gcccatactt atatggcggc
    14341 agaggcgctg gaaagcgccg cgaaagcgaa aggctgggaa gtgaaagtag aaacccaggg
    14401 gtcgattggt ctggaaaatg aactgactgc ggaagacgtg gcaagcgccg atatggtgat
    14461 tttgaccaaa gatatcggca tcaaatttga agagcgtttt gctggcaaga ctatcgtgcg
    14521 tgtcaacatc agcgacgcag taaaacgtgc tgacgccatt atgagcaaaa ttgaagctca
    14581 cctggcacaa accgcttaag ttcttatccc gcccgcaagg gcgggttcgc tttcccacag
    14641 gagttcctca tgacgaatcg tatctctcgc ctcaaaactg cactgtttgc caatacccgt
    14701 gaaatctcgc tggagcgggc gttgctttat accgccagcc atcggcaaac cgaaggggag
    14761 ccggtgataa tgcgtcgggc gaaagcaaca gcgtatatcc ttgaacacgt tgaaatttcg
    14821 attcgtgatg aagaactgat tgccggtaac cgcaccgtaa aaccgcgcgc cgggattatg
    14881 tcgccggaaa tggaccctta ctggctgctg aaagagctgg atcaattccc gacgcgtccg
    14941 caggaccgct ttgctatcag cgaagaagat aaacgtatct accgcgaaga gttgttcccg
    15001 tactgggaaa aacgttcgat gaaagatttc atcaacgggc agatgacgga tgaagtaaaa
    15061 gccgcgacca gcacgcagat tttcagcatc aaccagacgg ataaaggcca ggggcacatt
    15121 attattgatt acccgcgcct gctgaatcac gggctggggg agctggtggc acagatgcag
    15181 caacattgtc agcaacagcc ggagaatcac ttttatcagg cagcgctgtt actgctggaa
    15241 gcctcgcaga aacatatttt gcgttacgcc gaactggcgg aaacgatggc ggcaagctgc
    15301 actgatggcc agcgtcgcga agaactgctg accattgcgg agatctcccg ccataacgcc
    15361 gaacataagc cgcagacgtt ctggcaggcg tgccagttat tctggtacat gaacatcatt
    15421 ctgcaatacg aatccaacgc cagttcgcta tcgttggggc gcttcgacca gtatatgttg
    15481 ccgttctatc aggcatcatt aacccaggac gaagatccgg cattcctgaa agaactgctc
    15541 gaatctttat gggtgaaatg caacgacatc gtgctgttgc gctccaccag tagcgcgcgt
    15601 tatttcgcag gtttccctac cggctatacc gcactgctcg gcgggttaac cgagaacgga
    15661 cgtagcgcgg tgaacgtgct ttcgttcctt tgccttgacg cttatcaaag cgtgcaatta
    15721 ccgcaaccga acctcggcgt gcgcactaac gccttgatcg acacgccgtt cctgatgaaa
    15781 accgcagaaa ccattcgcct cggcaccggt attccgcaaa tctttaacga tgaagtggtg
    15841 gtgccagcgt tcctcaaccg tggcgtttcg ctggaagatg cgcacgacta ttccgtagtg
    15901 ggctgtgtgg aattatctat tcccggcaga acctacggct tgcatgacat cgcgatgttt
    15961 aacctgctga aagtgatgga aatctgcctg catgaaaatg aaggcaacgc tgcgctgact
    16021 tatgaaggtt tactggaaca gatccgcgcc aagatcagcc actacatcac cctgatggtt
    16081 gagggcagca atatttgtga tatcggccat cgcgactggg ctcctgtacc gctactctcg
    16141 tcttttatca gcgattgtct ggaaaaaggt cgcgatatta ccgatggcgg cgcgcgttat
    16201 aacttctccg gcgtacaggg gatcggtatc gccaacctga gcgattctct ccatgcgttg
    16261 aaagggatga tttttgatca acagcgttta agttttgacg aattgctgtc ggtattaaaa
    16321 gccaacttcg ccacgccaga aggcgaaaaa gtccgcactc gcttaattaa ccgcttcgag
    16381 aaatacggta acgatatcga cgaggtggat aacattagcg ccgaactgtt acgccactac
    16441 tgcaaagaag tggaaaaata ccagaacccg cgcggcggct acttcacgcc gggatcgtat
    16501 accgtttctg cccacgtccc gttgggatcg gtggttggcg cgacgccaga cggtcgtttt
    16561 gccggggaac aactggcaga cggtggcttg tcacccatgc tgggtcagga tgcacaaggg
    16621 ccaacggcgg tactgaaatc agtcagtaag ctcgataata cgctgctgtc taacggtacg
    16681 ttgctgaacg tgaaattcac tccggcgacc ctggaaggtg aagcgggatt acgcaaactg
    16741 gccgacttct tacgggcgtt tacccagctt aagttgcagc atattcagtt taacgtggtg
    16801 aacgccgaca cgttgcggga agcgcaacag cgcccacaag attatgccgg gctggtggtg
    16861 cgcgttgccg gatacagcgc cttctttgtc gaactgtcga aggagatcca ggatgacatc
    16921 atccgccgga cagcgcatca gctgtaacgt tgtggaaacg cgccgcgatg atgtggcgcg
    16981 cattttcaac attcagcgtt attcactgaa tgacggcgag ggcattcgta cggtggtctt
    17041 ttttaaaggc tgtccgcatc tttgcccatg gtgtgctaat ccggagtcga tctccggcaa
    17101 aatccagacg gtgcgcagag aggcgaaatg tctgcactgc gcgaaatgtt tgcgtgatgc
    17161 ggatgaatgc ccctccgggg catttgaacg gattggccgc gatatcagtc ttgacgctct
    17221 ggaacgggaa gtgatgaaag atgacatttt ctttcgcacg tccggcggtg gcgtcacgct
    17281 ttctggcggc gaagtgttaa tgcaggcgga gtttgcgacc cgttttttac agcgactgcg
    17341 gctatggggt gtgtcatgcg ccatcgaaac tgccggagac gcgccagcca gcaagctgtt
    17401 accgctggcg aaattgtgcg atgaagtgtt gttcgattta aaaattatgg atgcggctca
    17461 ggcgcgggat gtggtgaaga tgaacctgca acgcgtgctg gagaatctgc gtttgctggt
    17521 gagtgagggt gtcaacgtga tcccgcgttt accgctgatc cctggtttca cgctcagccg
    17581 ggagaatatg cagcaggcgc tggacgtgct gatcccgctg aatatcaggc agatccatct
    17641 gttaccgttt catcagtacg gcgagccgaa ataccgcctg ctggggaaaa catggtcgat
    17701 gaaagaggtt gctgcgccgt cgtcagccga tgtggcaacg atgcgtgaaa tggcagaacg
    17761 ggccggattt caggttaccg tgggaggtta aaatggcata cctggtggca gtaaccgcct
    17821 gcgtaagcgg cgtggcgcat acttatatgg cggcggaacg gctggaaaag ttgtgccagt
    17881 tagagaagtg gggcgttagc attgaaactc agggcgcgtt gggaacggag aatcgtttag
    17941 cggacgagga tatccgccgg gcggatgttg cgctgttaat tacggatatc gagcttgccg
    18001 gtgctgagcg atttgaacat tgtcgctacg tgcaatgcag catctacgct ttcctgcgtg
    18061 agccgcagag ggtgatgagc gcagtgcgca aagtgctttc tgcgccgcag caaacccatc
    18121 ttattctgga gtagtcggtt tttctgtcag ttggctgtgg tactgccgac gatattccga
    18181 cggcgagcgt tcggtatttt tacgaaacag acggcagaag tagttgctgt cgacaaagcc
    18241 gcaggcgtgc gccacttctt ttaccttcag gtcatagcct ttcaataacg tcttagcgtg
    18301 ctccagtcgc gtgtgattca ggtattcgtt aaaaccaatg gcccccgttt tttgaaacag
    18361 gtgcgagagg taatttggcg aaatatagaa tgcctgtgcg acagattcgc gggtaagcgc
    18421 ggaggcatag cgttcgtcga tataatcacg aatagcttcg aacagtgcct ggctgcgtga
    18481 ggcggtctgg atttggctgc cgagcaaatc gcggcagtgg ctaagcaggc tggcgacgat
    18541 aagtctggcc gtttgctgct cctgcgactg catttgcatt tcattgagcg tttgtagcag
    18601 aaaagaacca atgcgcgggc cgcgtcgggc gacgtgttgc ttcgccagat tttgatattg
    18661 tttgccatcc cattgcacaa cgctgaagcc gagttgctgt ttgccaaaca gcacgctaaa
    18721 ggtggtggcg ggggcttgcc attgtggaaa attccagcca ccagcaggga cgtacagcac
    18781 atcgccggga actaacgctg ctccagcgcc ggtatcaata aactcacctt ccagcacaat
    18841 ttccagccgt ggaaaatcga cctgatacgc aagatcgggt accgggccgt tgctgctggc
    18901 aaagtaaatc tggcgcaggg acagcgggcc gttgatcagg cgggagagca gatagctgac
    18961 gtcgtgatac atgttattcc cttaggtatg tagacgggaa aacagtaact ggcattatga
    19021 aaaaaggcca cctgaaaagt gtggcctgat gggagtgaga tgtaaacatt gcggcagttt
    19081 cagtcgactt gtattgccgg atgcggcgta aacgccttat ccggcctacc gccgcgatct
    19141 gtaggccgga taagacgctc aagcgtcgca tccggcaaat aacatctggc tgataattac
    19201 tgattaccca cctgatcgcc atacggcaga gtgtcgtaat cgatcagcgc gtttttcttg
    19261 tacgggttac caatccagcg ggtagtttct ttgaactgcg gattcaccac tgaacgggtt
    19321 ggatcgtaac cgtcgtaaga taagcccgct aaatctgacc aggtgtggat cagttccgcc
    19381 aggctgtatt tacgatcaac atcctgtgag aaatcacgcg gatgagtcgc ttgccatttt
    19441 tctgacgtcc acagcaggaa cggaatggtg tacatatgac gcgtcgggtt gtcttcatta
    19501 cgcccctgag ttttgtgcgg cggcgtgtcg taaacctctt caccgtggtc agagaaataa
    19561 accaggaaac cgttcggatc ggtcgcttta aagtctttaa tcaggctggc aaccacgtga
    19621 tcgttataca agttagcgtt gtcataatcg ttatatgact ccagttcttc tgcgcttaat
    19681 cccggcggaa catgatcggt attgccatca aacttgccct gattttccgg gtagcggtat
    19741 ttgtatttga tatgcgtacc cagcagatga acgatgatca gtttcttcgg cgcagggtca
    19801 ttcagcactt cctggaacgg cttcagcacg ttggtgtcgt attcacgcgc actctgcgta
    19861 cgttgctggt tcatgtagta ctgcttgtcg gtctggcgcg aaaataccgt cagcatggta
    19921 ttgcgggcgg tcatcgtctg ctggttggtg atccagaagg ttttataacc cgcctgtttc
    19981 atcatgttca tcagcgacgg ctgcgtcaga tacagatccg ggttcttttc attggcaaag
    20041 gtcagcgcct gttgcaggat ttcaatggtg tacggacgag acgtcaccac gttattaaac
    20101 acggtcagat tcggatcggt tttatgcagc gcatccagct ccggcgtggt ttcacgcgga
    20161 taaccgtaca gactcatgcg gccacgctgg gtcgactcgc caatcaccag cactaaagtg
    20221 cgcggttcgt tacccgattc atctttgaaa ttcgccagcg gcggcaaggc attattttca
    20281 ttcagtagct ttgttagcga gtttagttgc tgacgatact gataatagcc ggtcaggaat
    20341 tgccacggtg cggcaggctc catacgcgat gccaggttat ccaacgtttt ctcaaatggc
    20401 ttgtttttga taaacgtatt catggcgatc ggatgcagaa tcaagccata aagtagggca
    20461 aaagagacaa cataacgcca cggctttgga atatagaccg ggcgcaggcg cgtccacagc
    20521 aaaactgcca ccgccgtata ggccagcgcg ataagcacaa tcttcaggct gaaatactgg
    20581 cttaaatact cgctggcttc gttggtgttg gtttcaaaca tcacaaacag aacgctctgc
    20641 gagaactcct gaccgtagat gacgtagtag cacaacgctg ccagagaggc cgcccatagc
    20701 accacgccga tcactgcggc aataatttta atccgcttcg gaaagaggaa taccgggatc
    20761 aaccacagcg aactgaataa cagcgagtcg cgaatgccgt tagtgccact ataaccactg
    20821 atgtaaataa tggcctgtag cagagtagag aaaaaccaaa agtagagcag tgcccaaccc
    20881 agggctttcc agctaaaaag aggtttagcc tggacttctg tggaatgcat agtaagaacc
    20941 tgtcttgaaa aaaatatcgc cgaatgtacc gacaattcct taaggatatc tgaagggata
    21001 tccagaattt gaataaaatg cagacagaaa tatattgaaa acgagggtgt tagaacagaa
    21061 gtatttcaga aaaccctcgc gcaaaagcac gagggtttgc aggagaggaa gattagccgg
    21121 tattacgcat acctgccgca atcccggcaa tagtgaccat taacgcctgc tcgacgcgag
    21181 gatccggctc atggccttct ttttctgcct ggcgggagcg gtgcagcaac tcggcctgca
    21241 atacgttcag cgggtcggtg taaatattcc gtagctgaat agactctgca atccacggca
    21301 gatcggccat cagatgggaa tcgttggcaa tcgccagcac cactttgatg tcttcttctt
    21361 gcagattgcg taactcttta cctaacggcc acagtgcttt gtctaccaga cgttgatcat
    21421 agtattccgc cagccacagg tctgctttgg cgaagaccat ctccagcatg ccgagacgcg
    21481 tcgagaagaa tggccaatcg cggcacatag cctccagctc gctctgtttg ccatcttcga
    21541 ccactttttg cagcgccgta cctgcaccca gccaggcggg gagcatcagg cggttttgcg
    21601 tccaggcgaa gatccacgga atggcgcgta gtgactcgac gccgccggtt gggcgacgtt
    21661 tcgccggacg tgaacccaac ggcagtttgc ccagttcttg ttccggcgta gcggagcgga
    21721 agtaaggcac aaaatctttg ttttcacgta cgtagccacg gtagagatcg caggagatga
    21781 ctgacagttc atccataatg cgacgccagc tctctttcgg ctcaggtggt ggcagcaggt
    21841 tggcttccag aatcgccccg gtgtacagcg acaggctgct gacggtgatt tctggcagac
    21901 catatttaaa gcggatcatc tcgccctgtt cagtcacgcg caggccacct ttcaggcttc
    21961 ctggcggttg tgacagcagc gccgcatgag caggtgcgcc gccgcgacca atggaaccgc
    22021 cgcgaccgtg gaacaacgtc agctcaatac ccgctttttc gcaggttttg attaatgcat
    22081 cctgtgcctg atattgcgcc caggaagctg ccatcacgcc cgcatctttt gctgagtcgg
    22141 aatagccaat catcaccatc tgtttgccct gaatcaggcc gcgataccag tcgatattca
    22201 gtagctgagt catgacatcg ttggcgttgt tcaggtcatc gagggtttca aacagcggag
    22261 caaccggcat cgcaaacccg atacccgctt ctttcagcag caggtggaca gccagcacgt
    22321 cggacggcgt tttcgccatc gagatcacgt aggcggcaat ggagccttgc ggcgcttcgg
    22381 caatcacctg gcaggtatcg agcacttcgc gcgtttcggc gcttggttgc cagttgcgcg
    22441 gcagaagcgg acgtttggag ttcagttcgc ggatcaggaa cgcctgtttg tcggcctctg
    22501 accagctttc gtagtcgccg ataccgaggt agcgggtcag ctcgcccagc gcttcggtat
    22561 ggcgggtgct ctcctgacgg atatcaatac ggaccagcgg tacgccgaaa catttcacgc
    22621 ggcgcagggt gtcgagcaga tcgccgttgg cgataatacc catgccacac gcctgaagtg
    22681 actggtagca agcgtagagc ggttcccaca gttcttcgtt ttgtgtcagc aggccttctg
    22741 gttttggcag ttcttcgcct ttcaggcgcg cttccagcca tgcctgtgtc gccatcaggc
    22801 gagagcgcag gtttttcatc agatagcgat acggctctgc ggcaccttct tcgccaacca
    22861 gcgccagcag ttcaggggtc gcttcaacca tcgacagttc agaaaccagc acctgaatat
    22921 ctttcaggaa caaatcggtg gctttccagc ggctgagtag caggacgtgg cgggtgatat
    22981 cggcagtgac gttcgggttg ccgtcgcggt caccgcccat ccacgaggta aaacgtaccg
    23041 gaacaaattc gacgggcagt ttgtagccga ggttctcttc cagttgttcg ttcagttcgc
    23101 gcaggtaatt cggtacgcct tgccacaggc tgttttctac tacggcaaag ccccatttgg
    23161 cttcatctac cgggcttgga cgcagcttac ggatttcatc tgtatgccat gactgggcga
    23221 tcaactggcg caggcggcgc atcagctggt tatgttcgta gtcggcgata tctttgttat
    23281 cgagctgttt taagcaggcg ttcacttcca ccattttgtg gatcagtgta cgacgggtaa
    23341 tttcggttgg gtgagccgtg aggaccagtt ccagcgacag cgattccact gcttttttga
    23401 tggtgtcttc gctcagttcc ggctggtttt tcagtttacg cagggtgcgg gcgatcactt
    23461 ccgggttgct ggcagcttcg cctttcggcg aaatgctgtg gtattgctcg gcggtgttgg
    23521 ccaggttcag aaactgacta aacgcacgcg caacgggcag cagctcgtcg ttcgacaaat
    23581 tttgtaaggt ggtgagcaac tcctggcggt tagcatcatt gccagcgcgt gaagatttgg
    23641 acaacttacg gatagtttct acgcgttcaa gaatgtgttc tcctaacgca tccttgatgg
    23701 tttctcccag cactttgccg agcatactga cattactacg caatgcggaa tattgttcgt
    23761 tcatattacc ccagacaccc catcttatcg tttgatagcc ctgtatcctt cacgtcgcat
    23821 tggcgcgaat atgctcgggc tttgcttttc gtcgtctttt ataaagccac gtaaaagcgg
    23881 tgacgtcaaa tgctgcgaaa tcgcttcagc aaacgaataa atagcaggaa tttacgccat
    23941 taaattcacg acgctttaaa taagcgtaac ttatgggaat gttaaaaaat cgcctcgagt
    24001 aacaccaaag gtgtaggtcg gataagatgc gtaagcatcg catccgacat tgtttggcct
    24061 acgttttaat gccagcaaaa atggtgaatc acctgggtta tcagctcacg ggtgggcttg
    24121 ataaaccgcg tctccagata ttcatcaggc tgatgagcct gattaattga gccaggcccc
    24181 agaaccagcg tcgggcataa cgtttgaata aacggcgctt cggtacagta attcaccact
    24241 tcggtctttg ctccgagcaa tttctcaact acttcaacca gttgatgatt tggcgggcat
    24301 tcatagccag ggatcggcgg atgcagctcg tcgaccgtca gacgacccgg ccagcgttcg
    24361 ctcaccggag ccagcgcatc gttgagtagg ccattaagtt cattgagtgt catgcccggc
    24421 agcggacgaa tatccatatg caactcacag caagcacaaa tacggttaga agcgtcgcca
    24481 ccgtgaatat gcccgaggtt gagcgtgggg tatggcacgg taaacgcttc gtagtgataa
    24541 cgttctttca ggttatcgcg caattgcaaa atatgcccga tggcgtcgtg cattagttcg
    24601 atagcgttaa ctccgcgtgc tggatcgctg gagtgccccg actggccctg aatacggatg
    24661 gcgttagaga tatgaccttt atgtgcgcgt accggttgta gtgacgtcgg ttcgccaatg
    24721 atggcgcaat ccgggcgcag ggcggtagtt tcggcaaaat aacgcgctcc ggccatactg
    24781 gtttcttcat cggcagtcgc cagaatgtag agcggttttt tcagtttcgt gacgtcgaca
    24841 tcgcgtagcg catcaaggat aaacgcaaaa aagcctttca tgtcggcggt gcctaagccg
    24901 taaagcttgc cgtcatgctc cgtcaacgta aatggatcgc gcgtccagcg tccgtcatca
    24961 aatggcaccg tatcggtatg ccccgccagc aacaaaccgc cagccccctg tccgcaactg
    25021 gccagcatat taaatttgtt gcgagttcct ggcacaggct gtacttccac attgaagcct
    25081 aaatccttaa accagtccgc cagcagagtg attaaatctg cattgctttg atcgagtgcc
    25141 tcttccgtgg cgcttattga aggtgtggca atcagagcac ggtaaatctc gataaatggc
    25201 ggtaatttgt ttttcattgt tgacacacct ctggtcatga tagtatcaat attcatgcag
    25261 tatttatgaa taaaaataca ctaacgttga gcgtaataaa acccaccagc cgtaaggtga
    25321 atgttttacg tttaacctgg caaccagaca taagaaggtg aatagccccg atgttgaata
    25381 cgctgattgt gggtgccagc ggctacgctg gcgcagagct agtgacctat gtaaatcgcc
    25441 atccgcatat gaacataacc gctttgactg tttcagcgca aagcaatgat gcgggaaagt
    25501 taatctccga tttgcatccg cagctaaaag gcatcgttga tctgccgttg cagccgatgt
    25561 cggatatcag cgagtttagc ccaggggtgg acgtggtgtt tctcgccacc gcccatgaag
    25621 tcagccacga tttagcgccg caatttcttg aagcaggctg tgtggtgttc gacctttccg
    25681 gcgcgtttcg tgttaacgat gtcgccttct atgaaaaata ttacggcttt acccatcaat
    25741 acccggaact gctggaacag gcagcctacg gtctggcgga gtggtgcggt aataaattaa
    25801 aagaagcgaa cttgattgcc gtgccaggct gttatccgac ggcagcacag ctggcgctga
    25861 aaccgttgat tgatgccgat cttcttgacc tcaatcagtg gccggtgatc aacgccacca
    25921 gcggcgtgag cggtgcagga cgtaaggcag cgatttcaaa tagtttttgt gaagttagcc
    25981 tgcaaccgta tggcgtcttt acccatcgcc atcaaccaga gatcgccaca cacctcggtg
    26041 ctgacgttat cttcaccccg catctgggca atttcccgcg cggcattctc gaaaccatta
    26101 cctgccgcct gaaaccgggt gtgagccagg tgcaagtggc gcaagcgtta cagcaggcgt
    26161 atgcccataa accactggtg cggctgtatg acaaaggcgt tccggcgctg aaaaatgtcg
    26221 ttggactgcc attctgcgat atcggttttg ccgttcaggg cgagcatctg attattgtgg
    26281 cgaccgaaga caacttactg aaaggcgcgg cggcacaagc ggtgcagtgc gccaatattc
    26341 gtttcggcta tgcggaaacg cagtctctta tttaagggtg caatgatgaa tccattaatt
    26401 atcaaactgg gtggcgtact gctggatagt gaagaggcgc tggaacgtct gtttagcgca
    26461 ctggtgaatt atcgcgagtc acatcagcgt ccgctggtga ttgtgcacgg cggcggttgc
    26521 gtggtggatg agctgatgaa agggctgaat ctgccggtga aaaagaaaaa tggcctgcgg
    26581 gtgacgcctg cggatcagat agacattatc accggagcac tggcgggaac ggcaaataaa
    26641 accctgctgg catgggcgaa gaaacatcag attgcggccg taggtttgtt tctcggtgac
    26701 ggcgacagcg tcaaagtgac ccagcttgat gaagagttag gccatgttgg actggcgcag
    26761 ccaggttcgc ctaagcttat caacagtttg ctggagaacg gttatctgcc ggtggtcagc
    26821 tccattggcg taacagacga agggcaactg atgaatgtca atgccgacca ggcggcgacg
    26881 gcgctggcgg caacgctggg cgcggatctg attttgctct ctgacgtcag cggcattctc
    26941 gacggcaaag ggcaacgtat tgccgaaatg accgccgcga aagcagaaca actgattgag
    27001 cagggcatta ttactgacgg catgattgtg aaagtgaacg cggcgctgga tgctgcgcgc
    27061 acgttgggcc gtccggtaga tatcgcctcc tggcgtcatg cggaacagct tccggcactg
    27121 tttaacggta tgccgatggg tacgcggatt ttagcttaag ttttgttggc cggaggcgca
    27181 gctttccggc attgaatttc aaaataagga aacagagtta tggcactttg gggcgggcgt
    27241 tttacccagg cagcagatca acggttcaaa caattcaacg actcactgcg ctttgattac
    27301 cgtctggcgg agcaggatat tgttggctct gtggcctggt ccaaagccct ggtaacggtc
    27361 ggcgtgttaa ccgcagaaga gcaggcgcaa ctggaagagg cgctgaacgt gctgctggaa
    27421 gatgttcgcg ccaggccaca acaaatcctt gaaagcgacg ccgaagatat ccatagctgg
    27481 gtggaaggca aactgatcga caaagtgggt cagttaggca aaaaactgca taccgggcgt
    27541 agccgtaatg atcaggtagc aactgacctg aaactgtggt gcaaagatac cgttagcgag
    27601 ttgctgacgg ctaaccggca gttacaatcc gctctggtcg agaccgcgca gaacaatcag
    27661 gacgcggtga tgccgggtta cactcacctg caacgcgccc agccggtgac gttcgctcac
    27721 tggtgcctgg cctacgttga gatgctggcg cgtgatgaaa gccgtttgca ggacgcgctt
    27781 aagcgtctgg atgtcagccc gctaggctgt ggcgcactgg caggaacggc ctatgaaatc
    27841 gaccgtgaac agttagcagg ctggctgggc tttgcttcgg cgacccgtaa cagtctcgac
    27901 agcgtttctg accgtgacca tgtgttggaa ctgctttctg ctgccgctat cggcatggtg
    27961 catctgtcgc gttttgctga agatctgatt ttctttaaca ccggcgaagc ggggtttgtg
    28021 gagctttctg accgtgtgac ttccggttca tcattaatgc cgcagaagaa aaacccggat
    28081 gcgctggagc tgattcgcgg taaatgcggt cgggtgcagg gggcgttaac cggcatgatg
    28141 atgacgctga aaggtttgcc gctggcttac aacaaagata tgcaggaaga caaagaaggt
    28201 ctgttcgacg cgctcgatac ctggctggac tgcctgcata tggcggcgct ggtgctggac
    28261 ggcattcagg tgaaacgtcc gcgttgccag gaagcggcgc agcagggtta cgccaacgct
    28321 actgaactgg cggattatct ggtggcgaaa ggcgtaccgt tccgcgaggc gcaccatatt
    28381 gtgggtgaag ctgtggtgga agccattcgt cagggcaaag cgttggaaga tctgccgctc
    28441 agtgagttgc agaaattcag ccaggtgatt ggcgaagatg tctatccgat tctgtcgctg
    28501 caatcgtgcc tcgacaagcg tgctgcaaaa ggcggcgtct caccgcagca ggtggcgcag
    28561 gcgattgctt ttgcgcaagc tcggttagag tagtttgttt atattgctcc cctgaaaatg
    28621 gggagcttac cacgcctaca aagtgcgaca cttcgtaggc ggataaggcg tttacgccgc
    28681 atcaggcaga aatttgtcag tattctggtg accttaaatt taacgcccaa ccagaaccat
    28741 tatttgtcac attccttttt attcttgcgt cttctttacc ttcactctcc ccataaaaat
    28801 tatattttca atacatcagc caatacatac tttaattaat tgaatataat tcatttctat
    28861 tgctgtgttc atttttaatg attaatggag tttgttatgt gattatttaa cgtgttgttt
    28921 taacgatgat agctaattat tcaggtggtt tttgagggtg ttttttctcc tcggtagaaa
    28981 tttttatttt taatgtgatc atggttgaat aatcgctctt tctctcttgt atggtagtag
    29041 gcgcaaaatt aagtttgaaa cgtctattta caacacagga gattgcaata atgaaaatta
    29101 ttgctgcaga tgtttttgtt acttgcccag ggcgtaattt cgttacgctt aaaataacca
    29161 ctgcaagcgg actttgtgga ttaggagatg ccacgttaaa tggtcgcgaa ttgtcggtcg
    29221 cttcttatct gaaagatcat ctttgtccac aactaattgg ccgcgatgcc agccgtatcg
    29281 aagatatctg gcaattcttt tataaaggtg cttactggcg acgtggcccc gtcacgatgt
    29341 cagccatttc tgccattgat atggcgttgt gggatatcaa agcaaaagcg gcaaatatgc
    29401 cattgtatca actgcttggt ggtgcttctc gtgaaggcgt tatggtttat tgccacacca
    29461 ccgggcgtac gattgatgaa gtccttgaag actatgcaaa gcaccagcag atgggcttta
    29521 aggcgattcg tgtgcagtgt ggtgtaccgg gtatgcaaac cacctatggt ctggcgaagg
    29581 gcaaaggact ggcgtatgaa ccagcaacca aaggcctgtg gccagaagag cagttgtggt
    29641 cttcagaaaa gtatctcgat tttacgccga agttatttga agcggtgcgt aataagttcg
    29701 gttttaacga acatctgttg catgacatgc accatcgcct gacccccatc gaagcggcgc
    29761 gctttgggaa gagcattgaa gattaccgcc tgttctggat ggaagatccg acgcctgccg
    29821 agaatcagga atgttttcgt ctgattcgtc agcataccgt gacgccgatt gcggtcggtg
    29881 aagtgtttaa cagtatctgg gactgcaagc agttaatcga agagcagctg attgactata
    29941 ttcgcacgac gattacccat gcaggcggca ttaccgggat gcgacggatt gccgattttg
    30001 catcgcttta tcaggtacgt accggttcgc atggcccttc agatttatcg cctgtttgcc
    30061 atgcggcggc gctgcatttt gacctctggg tgccaaactt tggcgttcag gagtacatgg
    30121 gctattccga acaaatgctt gaagtcttcc cgcatagctg gcgcttcgat gaaggttata
    30181 tgcacccagg cgatgaaccg gggctgggta tttctttcga tgagaaactg gcggcgaaat
    30241 atccgtatga cccggcgtat ctccccgtcg cacgactgga agatggcaca ttgtggaact
    30301 ggtaatagcg gttcgcttaa ttattttctg aatattcatc aaatcgatta attccgctca
    30361 aggagctaaa aatggacgtt gatgtgtcaa ccagtgttgc gggtaataaa ccgcaacgga
    30421 ttcgtcgtat tcagacagtg accctggttt tattatttat ggcggggatc gttaattttc
    30481 tcgaccgttc gtcattgagc gtggcaggtg aagcaattcg tggcgagtta ggattatccg
    30541 ctaccgagtt tggtgttttg ctttctgcat tttctctgtc ttatggtttt tcacaacttc
    30601 cttccggtat tttgctagac cgctttggcc cacgaatagt gttaggcgca ggcttaatat
    30661 tctggtcatt aatgcaggca ttaacaggaa tggttaattc ttttagccac tttattttaa
    30721 tgcgtatcgg tctgggtatt ggcgaagcgc catttatgcc tgcgggagta aagtcgatca
    30781 ccgactggta tgcgcaaaaa gaacgcggca cggcgctggg gatttttaac tcatctaccg
    30841 ttatcggtca ggctatcgcg cctcctgctc ttgtattgat gcaactggca tggggctggc
    30901 ggacgatgtt tgtcatcatc ggcgtagcag ggattctggt tgggatctgt tggtacgcgt
    30961 ggtatcgcaa ccgggcgcag tttgtcctga ccgaggaaga acggacatat ctctccgcgc
    31021 cggttaaacc gcgtccacag ttacaattta gcgagtggct ggcgctgttt aagcatcgga
    31081 caacctgggg gatgatttta ggtttctctg gcgtcaatta taccggttgg ctctacatcg
    31141 cgtggttacc cggttatttg caggccgaac aaggtttcag tctggcgaaa accggttggg
    31201 tggcggcaat tcctttcctc gcggcggcgg tcgggatgtg ggttaacggt attgttgtcg
    31261 atcggctggc gaaaaaaggc tatgacctgg cgaagacgcg taaaacggcg attgtctgcg
    31321 gtttgatgat gtcggcatta ggcacgttgc tggtcgtgca atcttcctcg ccagcccagg
    31381 cggtagcgtt tatctcaatg gcgctgttct gcgtgcattt cgcgggaaca tctgcatggg
    31441 ggctggtgca ggtgatggtg tcagaaacaa aagtggcttc catcgccggg attcaaaact
    31501 ttggcagttt tgtttttgct tcctttgctc cgatcgtaac cggttgggtc gtggatacca
    31561 cacactcgtt taatctggcg ctggttatcg cggcctgtgt gacgttcacc ggggcgctgt
    31621 gttacttctt tattgtcaaa gatcggattg agtaaccgtt actatcaggc tctcttgctg
    31681 tgggcctgta gaataaaaaa aagcggatcc tggagatccg caaaagttca cgttggcttt
    31741 agttattcga gttgagaaac tctcgaaacg ggcagtgact tcaagggtta aaagaggtgc
    31801 cgctccgttt ctgtgagcaa ttatcagtca gaatgcttga tagggataat cgttcgttgc
    31861 tatgctacct atcgccgcga actatcgtgg aaatggagga tggataatga atattcgtga
    31921 tcttgagtac ctggtggcat tggccgaaca ccgccatttt cggcgtgcgg cagattcctg
    31981 ccacgttagc cagccgacgc ttagcgggca aattcgtaag ctggaagatg agttgggtgt
    32041 gatgttgctg gagcggacca gccgtaaagt gttattcacc caggcgggaa tgctgctggt
    32101 ggatcaggcg cgtaccgtgc tgcgtgaggt gaaagtcctt aaagagatgg caagccagca
    32161 gggcgagacg atgtccggac cgctgcatat tggtctgatt cccacggttg gaccgtacct
    32221 gctaccgcat attatcccga tgctgcacca gacctttcca aagctggaaa tgtatctgca
    32281 tgaagcacag acccaccagt tactggcgca actggacagc ggcaaactcg attgcgtgat
    32341 cctcgcgctg gtgaaagaga gcgaagcatt cattgaagtg ccgttgtttg atgagccaat
    32401 gttgctggct atctatgaag accatccgtg ggcgaaccgc gaatgcgtac cgatggccga
    32461 tctggcaggg gaaaaactgc tgatgctgga agacggtcac tgtttgcgcg atcaggcaat
    32521 gggtttctgt tttgaagccg gggcggatga agatacacac ttccgcgcga ccagcctgga
    32581 aactctgcgc aacatggtgg cggcaggtag cgggatcact ttactgcctg cgctggctgt
    32641 gccgccggag cgcaaacgcg atggggttgt ttatttgccg tgtattaagc cggaaccacg
    32701 ccgtaccatt ggtctggttt atcgtcctgg ctcaccgctg cgcagccgct atgagcagct
    32761 ggcagaggcc atccgcgcaa gaatggatgg ccatttcgat aaagtgttaa aacaggcggt
    32821 ttaagccatt tagcgcagct acccgatagg cttccgccat cgtcgggtag ttaaaggtgg
    32881 tgttgacgaa gtactcaata gtgttgccgc cacctttctg ttccataatc gcctgaccga
    32941 tatgaataat ttcggcagcg cgctcgccaa agcagtgaat acccagaatc tcttttgttt
    33001 cccgatggaa caaaattttc agcgtgccca cgttcatgcc gacgatttgt gcacgtgcca
    33061 gatgtttaaa ctgggcgcgg cccacttcat atggcacttt catcgcggtc agctgctgtt
    33121 cggttttgcc cacagagctg atttccggga tggtgtaaat accggtaggg atatcttcaa
    33181 tcagatgtgc ggtggcttca cctttcacca gcgcctgcgc ggcaatgcgc ccctggtcat
    33241 aggccgccga cgccaggctc ggataaccaa tcacgtcgcc caccgcgtaa acgtgcggct
    33301 gtgcggtctg atacatgctg ttgaccttca gctgtccgcg gctgtcagtt tccagcccaa
    33361 tgttctgtaa cgccagcgaa tcggtattac cggtgcgacc gttggcatag agcaggcagt
    33421 cagctttcag ttttttaccc gacttcaaat gcatgatcac accgtcgtca cagccttcga
    33481 tcttctcgta ctcttcgttg tgacgaatca ctacgccact gttccagaag tgataggaga
    33541 gagaatctga catctcttga tcgagaaatg ccagcaggcg atcgcgggtg ttgatcagat
    33601 ccacttttac atccataccg cggaagatcg acgcatattc acagccgatc actccagcac
    33661 catagataag tacatggcgc ggttcgtggt gcatgctaag aattgagtcg ctgtcgtaaa
    33721 tgcgtggatg cgtgaaatca acatctgttg gatgatatgg acgagagccg caagcaataa
    33781 caaatttttc agcggttagt gtctcgacgc tgccgtccgg gcaatccagc gccagcgtgt
    33841 gctcgtcaac aaagcgagcg ttcccctgca atatttcaca gtgattacgt tcgtaaaatc
    33901 cctgacgcat gcgcgtttgt tgattaatca cgttatcggc atggttaagg atatcggcaa
    33961 aagaagagcg gagcagtcgc gaatggtcgc tgtaaagtgg gttttgatta aattctataa
    34021 tgcggctgac ggcgtgacgg agagctttcg acgggatggt gccccagtgg gtgcaaccgc
    34081 cgccaacatt ttgataacgc tcgataactg cgacgcgcgc accttgctta accaggccca
    34141 ttgcagcgcc ttcgccgccg gggccggaac ctattactat ggcatcgtaa tcgtaggaat
    34201 gtggcatggt agggcttacc tgttcttata cataaaagca acagaatgat aacattttat
    34261 cgcgggtaag ccaattgatc cccgtcattt atctggctat atcgtgagcg gcctttgctt
    34321 tgtctgtttc ttacttttga cctgaacgtt ttattggatt tttatcgacg atactctccg
    34381 tttaagcggc aggtttccgt tgtacgtaaa agaaccggcc aaagaattgc agtaaatatg
    34441 ttttattgcg ttaccgttca ttcacaatac tggagcaatc cagtatgttc attctctggt
    34501 atagtgccag cagtactttt ggcaaggatt cagacatcgt gatgggcgta agagcgcaac
    34561 aaaaagaaaa aacccgccgt tcgctggtgg aagccgcatt tagccaatta agtgctgaac
    34621 gcagcttcgc cagcctgagt ttgcgtgaag tggcgcgtga agcgggcatt gctcccacct
    34681 ctttttatcg gcatttccgc gacgtagacg aactgggtct gaccatggtt gatgagagcg
    34741 gtttaatgct acgccaactc atgcgtcagg cgcgtcagcg tatcgccaaa ggcgggagtg
    34801 tgatccgcac ctcggtctcc acatttatgg agttcatcgg taataatcct aacgccttcc
    34861 ggttattatt gcgggaacgc tccggcacct ccgctgcgtt tcgtgccgcc gttgcgcgtg
    34921 aaattcagca cttcattgcg gaacttgcgg actatctgga actcgaaaac catatgccgc
    34981 gtgcgtttac tgaagcgcaa gccgaagcaa tggtgacgat tgtcttcagt gcgggggccg
    35041 aggcgttgga tgtcggcgtc gaacaacgtc ggcaattaga agagcgactg gtactgcaac
    35101 tgcgaatgat ttctaaaggg gcttattact ggtatcgccg tgaacaagag aaaaccgcaa
    35161 ttattccggg aaatgtgaag gacgagtaat gaaacaagca aatcaagata gaggtacgct
    35221 gctgctggcg ttggttgctg gcttatcgat taatggtact ttcgcagcgc tgtttagctc
    35281 cattgtgcca ttttctgtat tcccgattat ttccctgttg ctgacggttt actgcctgca
    35341 tcaacgttat cttaatcgca ccatgccggt aggcttgccg ggtctggcag ctgcctgttt
    35401 tattctcggc gtactgctgt acagcacggt agttcgtgcg gaatatccgg atatcggctc
    35461 taacttcttc ccggcagtac tctccgtcat tatggtgttc tggattggcg cgaagatgcg
    35521 taaccgtaag caggaagttg ctgagtaatc ggtatcatgc cggatgctgg tgcatccggc
    35581 atgggtttta cttcgcggtc agtaatacgc cgcactccat atggtgcgtg taggggaact
    35641 gatcaaacag agccagacgt tcgaccttgt gcgtctggct taatgtttcc agattcttgc
    35701 ataacgtttc cggattgcag gagatataca gaatacgtgg atacgcctgc accattttct
    35761 cggtttcact gtccagaccg ctgcgcggtg ggtcgacaaa aatggtttcg cactgataac
    35821 tctttaagtc gattccttgc aggcggttaa actcgcgtac gccattcatc gcctgagtaa
    35881 attcttctgc cgccatacga ataatttgta cgttatcaat atggttagcg gcaatgttgt
    35941 attgcgcggc agcgaccgac ggcttagcga tttcggtggc taatacccga tcaaaattgc
    36001 gcgccagtgc taatgaaaag ttaccgttgc cgcagtacag ctccagcaaa tcgccttttg
    36061 agcctttggt tacgtccagc gcccattcca gcatctgaat attcatcgcc gcgttcggct
    36121 gggtaaagct gttttctacc tgacggtaga tcatctcttt ccctgcgacc ggcagacgtt
    36181 catcgatgta atcctggtcc agctcgattt tggttttcgt tgcccgacca atcagatgca
    36241 cattcagatt ctgcgcgcgc agtgcatcgc gcagggcttc cgcctgctga cgccactcat
    36301 catccagctt tttatggtac agcaaggaaa caaccgcttg attgcttagc gtagtgaggt
    36361 aatcaatctg gaacaacttg tggcgcagaa tgggattatt acgcacaccc gcaatcatcg
    36421 ccgtcatcaa ctggttgata agttcactgg cggcggggaa gctatccaca cggatacgac
    36481 ttttggtttg ttgatcgaaa atgatgtggt acaggtcatc gccatcgtgc cagatgcgga
    36541 actccgcacg catccgataa tgactgaccg gcgagcgaaa cacttccgga accaggtcag
    36601 aaaacggtgc catcatactt tgcaaacgta ccactttttc ggctaactgc gcttcatact
    36661 gttctgttgg aaggtgttcg ggggtcatga tgtatcctga aaaattaaag tacgcggcga
    36721 ttgtagggat tgctcatcag atgtccagat cttgatgaat tcctatttgt gagctacgtc
    36781 tggacagtaa cttgttacaa cctgtagcat ccacttgccg gtcctgtgag ttaataggga
    36841 atccagtgcg aatctggagc tgacgcgcag cggtaaggaa aggtgcgatg attgcgttat
    36901 gcggacactg ccatccggtg ggaagtcatc atctcttagt atcttagata cccctccaag
    36961 cccgaagacc tgccggctaa cgtcgcatct ggttctcatc atcgcgtaat attgatgaaa
    37021 cctgcggcat ccttcttcta ttgtggatgc tttacaatga ttaaaaaagc ttcgctgctg
    37081 acggcgtgtt ctgtcacagc cttttctgct tgggcacagg ataccagccc ggatactctc
    37141 gttgttactg ctaaccgttt tcaggaacca ttaagtgccg tgcttgctcc tgtcactgtc
    37201 gtgacacgtg aggatattga acgatggcaa gtttcctctg taaatgatgt tctccgccgt
    37261 ttgcctggtg tggcaattgc acaaaatgga ggagaaggtc agctctcaac tatttttatt
    37321 cgaggcacga actctaacca tgcactggtt ttaattgatg gtgtacgtct gaatttagca
    37381 ggtgttagcg gatcggctga cctgagccag tttccgatcg ctttggtgca gcgtatcgaa
    37441 tatatccgtg ggccacgttc agcgatttat ggctcagatg ccattggcgg tgtgataaat
    37501 attattacct cccgtgagaa tactggcact gaaatttcag ccggatgggg aagtaatagt
    37561 tatcaaaatt acgatatttc cactcagcaa caaattggtg aaaatactca agtaacactt
    37621 cttggtgatt atgcctatac caggggattt gatgtcgttg cctatggcaa cactgggatg
    37681 cagccacagt cagacagaga cggtttttta agtaaaacac tctacggcaa acttgaacat
    37741 aacttctctg atacgtggag cggatttgtt cgtggctacg gctatgataa ccgaactaaa
    37801 tatgacgcct ggtactctcc tggttctccg ctgattgata cacgaaaact ttatagtcaa
    37861 agctgggatg cgggtcttcg ctacgctggc gaaacactgc aatctcaact tgtttccagt
    37921 tatagccata gtaaagatta caactacgat ccacattatg gacgctatga cgcatcagct
    37981 aacctggatg agatgaaaca atacaatctc cagtggacaa atagcgtcac agtcggtcac
    38041 ggtgatgttg gagctggtat cgactggcaa aaacaatcca ctactcctgg aactggatat
    38101 ttgccaaaag gttacgatca gcgtaacacg gggatttatc tgactggatt acaaaaagtt
    38161 ggcgatttta cgttagaagg cgcggtgcgt aatgacgaca actcgcaatt tgatcgccac
    38221 acaacatggc aatccagtgt gggatgggag tttattgaag gctatcgctt tatcgcttcc
    38281 tatggaactg catttaaagc gccaaatctc ggtcagttat atggttacaa aggtaatccg
    38341 aatctcgatc cagaaaaaag caaacagtgg gaaggggcgt ttgaaggctt aacagcagga
    38401 gtgaactggc gaatttcagg ataccgtaat gatatcagtg atatgattaa ttgtacccaa
    38461 aactgcgaaa gatattataa cgacgcaaag gtgcatatta agggtattga atctacagca
    38521 aacttcgaga ctggaccgct ggtgcatacc gttagctacg attatacgga tgccagaaat
    38581 gccttaactg acaaaccgtt agaacgtcgt cctaaacagc aagtaaagta tcagctcgac
    38641 tggcaagtgt tcgattttga ctggggtgta acttatcaat atctgggatc gcgatacgat
    38701 tcggattact catcctggcc ctataagtca gtcaaaatgg gcggtgtgag cttgtgggat
    38761 cttgcggttg cgtatccggt cacctctcat ctgacagttc gtggtaaaat agccaacctg
    38821 ttcgacaaag attatgagac agtctatggc taccaaactg caggacggga atacaccttg
    38881 tctggcagct acaccttctg aaccacgtcc caccgtgctg gtgtttgact ccggcgtcgg
    38941 tgggttgtcg gtctatgacg agatccggca tctcttaccg gatctccatt acatttatgc
    39001 tttcgataac gtcgctttcc cgtatggcga aaaaagcgaa gagtttattg ttgagcgagt
    39061 ggtggcaatt gtcaccgcag tgcaagaacg ttatcccctt gcgctggctg tggtcgcttg
    39121 caacaccgcc agtaccgttt cacttcctgc attacgcgaa aagttcgact tcccggttgt
    39181 tggtgtcgtg ccggcgatta aacctgctgc acgtctgacg gcaaatggca ttgtcggatt
    39241 actggcaacc cgcggaactg ttaaacgttc ttatactcat gagctgatcg cgcgtttcgc
    39301 taatgaatgc cagatagaaa tgctgggctc ggcagagatg gtcgagttgg ctgaagcgaa
    39361 gctgcatggc gaagatgttt ctctggatgc gctaaaacgt atcttgcgtc cgtggttaag
    39421 aatgaaagag ccgccagata ccgttgtact ggggtgtacc catttccctc tactacaaga
    39481 agaactgtta caggtgctgc cagagggaac ccggctggtg gattctggcg cagcgattgc
    39541 tcgccgaacg gcctggctgt tagaacatga agccccggat gcaaaatctg ccgatgcgaa
    39601 tattgccttt tgtatggcaa tgacgccaga agctgaacaa ttattgcccg ttttacagcg
    39661 ttacggcttc gaaacgctcg aaaaactggc agttttagac tgatttggtt gaatgttgcg
    39721 cggtcagaaa attattttaa atttcctctt gtcaggccgg aataactccc tataatgcgc
    39781 caccactgac acggaacaac ggcaaacacg ccgccgggtc agcggggttc tcctgagaat
    39841 tccggcagag aaagcaaaaa taaatgcttg actatgtagc gggaaagtgt atcatgcaca
    39901 ccccgcgccg ctgagaaaaa gcgaagcgg
//
LOCUS       DAPHCV010000035        39892 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.035, whole genome shotgun sequence.
ACCESSION   DAPHCV010000035 DAPHCV010000000
VERSION     DAPHCV010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.035"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(99..1118)
                     /locus_tag="RH033_001823"
     CDS             complement(99..1118)
                     /locus_tag="RH033_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HDV2991019.1"
                     /translation="MKSILIEKPNQLAIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GESFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKLCCKVL
                     LTFSE"
     gene            complement(1130..2344)
                     /gene="rspA"
                     /locus_tag="RH033_001824"
     CDS             complement(1130..2344)
                     /gene="rspA"
                     /locus_tag="RH033_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HDV2991020.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(2550..2876)
                     /locus_tag="RH033_001825"
     CDS             complement(2550..2876)
                     /locus_tag="RH033_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HDV2991021.1"
                     /translation="MIKTTLLFFATALCEIIGCLLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            3011..3352
                     /locus_tag="RH033_001826"
     CDS             3011..3352
                     /locus_tag="RH033_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HDV2991022.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAENNKLVIESGDSAQSR
                     QRAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            3387..3947
                     /gene="speG"
                     /locus_tag="RH033_001827"
     CDS             3387..3947
                     /gene="speG"
                     /locus_tag="RH033_001827"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HDV2991023.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(3950..4660)
                     /locus_tag="RH033_001828"
     CDS             complement(3950..4660)
                     /locus_tag="RH033_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HDV2991024.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAQT
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            4768..5073
                     /locus_tag="RH033_001829"
     CDS             4768..5073
                     /locus_tag="RH033_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HDV2991025.1"
                     /translation="MKRSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            5272..7698
                     /locus_tag="RH033_001830"
     CDS             5272..7698
                     /locus_tag="RH033_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HDV2991026.1"
                     /translation="MSKNEQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVYNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            7759..10182
                     /locus_tag="RH033_001831"
     CDS             7759..10182
                     /locus_tag="RH033_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HDV2991027.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDIISDNLRRILKEYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLATIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            10193..10810
                     /gene="dmsB"
                     /locus_tag="RH033_001832"
     CDS             10193..10810
                     /gene="dmsB"
                     /locus_tag="RH033_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HDV2991028.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            10812..11666
                     /locus_tag="RH033_001833"
     CDS             10812..11666
                     /locus_tag="RH033_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HDV2991029.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAANNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWYNGYTTLAFFLTVLLS
                     GPILAATILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCTGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            11709..12323
                     /gene="dmsD"
                     /locus_tag="RH033_001834"
     CDS             11709..12323
                     /gene="dmsD"
                     /locus_tag="RH033_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HDV2991030.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTRDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            12481..13773
                     /gene="clcB"
                     /locus_tag="RH033_001835"
     CDS             12481..13773
                     /gene="clcB"
                     /locus_tag="RH033_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HDV2991031.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLFAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAKHS"
     gene            complement(13726..14421)
                     /gene="bioD"
                     /locus_tag="RH033_001836"
     CDS             complement(13726..14421)
                     /gene="bioD"
                     /locus_tag="RH033_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDV2991032.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(14546..15766)
                     /gene="mlc"
                     /locus_tag="RH033_001837"
                     /gene_synonym="dgsA"
     CDS             complement(14546..15766)
                     /gene="mlc"
                     /locus_tag="RH033_001837"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HDV2991033.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(15901..16794)
                     /locus_tag="RH033_001838"
     CDS             complement(15901..16794)
                     /locus_tag="RH033_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2991034.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTEAGKQFLVDSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYNL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            16901..18154
                     /locus_tag="RH033_001839"
     CDS             16901..18154
                     /locus_tag="RH033_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2991035.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYVGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHHRLHA"
     gene            18552..18887
                     /locus_tag="RH033_001840"
     CDS             18552..18887
                     /locus_tag="RH033_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HDV2991036.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNAKAEQKAPEQKAQAAKKHAKKHSHQQP
                     AKPAAQPAA"
     gene            18980..19063
                     /locus_tag="RH033_001841"
     CDS             18980..19063
                     /locus_tag="RH033_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2991037.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            19163..19984
                     /locus_tag="RH033_001842"
     CDS             19163..19984
                     /locus_tag="RH033_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HDV2991038.1"
                     /translation="MRRTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKATLTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(20023..20352)
                     /gene="mdtI"
                     /locus_tag="RH033_001843"
     CDS             complement(20023..20352)
                     /gene="mdtI"
                     /locus_tag="RH033_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HDV2991039.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(20339..20704)
                     /gene="mdtJ"
                     /locus_tag="RH033_001844"
     CDS             complement(20339..20704)
                     /gene="mdtJ"
                     /locus_tag="RH033_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HDV2991040.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            21115..22149
                     /gene="tqsA"
                     /locus_tag="RH033_001845"
     CDS             21115..22149
                     /gene="tqsA"
                     /locus_tag="RH033_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HDV2991041.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLKAIEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(22174..23562)
                     /gene="pntB"
                     /locus_tag="RH033_001846"
     CDS             complement(22174..23562)
                     /gene="pntB"
                     /locus_tag="RH033_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HDV2991042.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23573..25105)
                     /gene="pntA"
                     /locus_tag="RH033_001847"
     CDS             complement(23573..25105)
                     /gene="pntA"
                     /locus_tag="RH033_001847"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HDV2991043.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            25629..26573
                     /locus_tag="RH033_001848"
     CDS             25629..26573
                     /locus_tag="RH033_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV2991044.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            26759..28141
                     /locus_tag="RH033_001849"
     CDS             26759..28141
                     /locus_tag="RH033_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV2991045.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWS
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPSIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            28178..28900
                     /gene="folM"
                     /locus_tag="RH033_001850"
     CDS             28178..28900
                     /gene="folM"
                     /locus_tag="RH033_001850"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HDV2991046.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIISYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLTDVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(28897..29232)
                     /locus_tag="RH033_001851"
     CDS             complement(28897..29232)
                     /locus_tag="RH033_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HDV2991047.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLASLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            29361..30080
                     /gene="rstA"
                     /locus_tag="RH033_001852"
     CDS             29361..30080
                     /gene="rstA"
                     /locus_tag="RH033_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HDV2991048.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDRAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATVTKGIQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            30084..31385
                     /gene="rstB"
                     /locus_tag="RH033_001853"
     CDS             30084..31385
                     /gene="rstB"
                     /locus_tag="RH033_001853"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HDV2991049.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGVAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAVESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            31461..32390
                     /gene="tus"
                     /locus_tag="RH033_001854"
     CDS             31461..32390
                     /gene="tus"
                     /locus_tag="RH033_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HDV2991050.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAELEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINQLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IATLPQDAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYIAD"
     gene            complement(32387..33790)
                     /gene="fumC"
                     /locus_tag="RH033_001855"
     CDS             complement(32387..33790)
                     /gene="fumC"
                     /locus_tag="RH033_001855"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HDV2991051.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRVFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRKRINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(33933..35579)
                     /gene="fumB"
                     /locus_tag="RH033_001856"
     CDS             complement(33933..35579)
                     /gene="fumB"
                     /locus_tag="RH033_001856"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDV2991052.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGVPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            35778..36953
                     /gene="manA"
                     /locus_tag="RH033_001857"
     CDS             35778..36953
                     /gene="manA"
                     /locus_tag="RH033_001857"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HDV2991053.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESNKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPDDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGHATLCKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            37054..38562
                     /locus_tag="RH033_001858"
     CDS             37054..38562
                     /locus_tag="RH033_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HDV2991054.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKNGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVDQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(38607..39872)
                     /gene="uidC"
                     /locus_tag="RH033_001859"
     CDS             complement(38607..39872)
                     /gene="uidC"
                     /locus_tag="RH033_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HDV2991055.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQPADDEAGLRIRLKNELRRA
                     DKPSAGTGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     QEKRDGSALGDRLNYWDVKMSIQYDFMLK"
BASE COUNT         9974 a        10266 c         9657 g         9995 t
ORIGIN      
        1 accaaatcct tagtgcttct ttcgtgtttt tctattgtca taatggttat ctctaaaaaa
       61 gaggtaagat gcgtactact tactcgccgt tattggtatt attcagaaaa agtgagtaag
      121 actttgcagc aaagtttttg atcctgttca aataaactaa tggcatcagc aacatgctgg
      181 aaatcaaacg tatgggtaat taatttttct ggtttaatta accctttact taaccagtcg
      241 ataacgaccg gaaatttatt tgcatttaag cgtgaagaga aaatagagag ttcttttccg
      301 gtaattcctt gctgaatcac ttcagacggt tcactggaga agcccatcaa tacaatacgt
      361 gccgctggag aagccagcgt tacagcctct ttcaggatag aaggatgaca agccgcatcg
      421 ataattaatg tcggcttgat gcctttttca gcgaaactct cgccaagcgg tgtctggctg
      481 ttattaatcg cccagtctgc cccgctctct ttcgcttttt ccagtcgttc atcaatgcga
      541 tcggcaacaa tcacattttt aacgttatag acgcctttta atacctgaac gatcgtcagg
      601 ccgattggac cggcaccgta aaccagaacg gtatcatttt cagtcggttg accatgaccg
      661 gtaacgttag ccgcaatggt aaaaggttcg atcatcaccg catattgatc ggccactgct
      721 tcaggaattt tccacgcatt ttttgccgga accacggcat attcactgaa accaccgtca
      781 gcgtgcacac ctaatacagc cagtgtcgta caaacgttcg gcttacctat agagcacgga
      841 tagcaatgcc cacagctgac caccggatcg acagcaacgc gttcaccgac tctggcgctt
      901 tccacgcctt cacccactgc atcaatgacg ccaaagaatt catgaccaat gacgcgcgga
      961 tatttcgcaa aaggattatg cccacgataa atatggctat ctgaaccaca aattccggca
     1021 agtttcactt ttactcgtac ttcacccgct gacggggtgg gtatttcgcg ttcgataatt
     1081 gccagttgat tcggtttttc aattaaaatg cttttcatta tcttactcct taccagttcc
     1141 acagcgtgcc atcttccaga cgtgcgactg gcagataagc aggttcatag ggatatttcg
     1201 ccgccagctt ttcatcgaat tcgatgccaa gacccggttt gtctcccgga tgcatatagc
     1261 cgttatcgaa agtccagttg tgcgggaaga cttcaagcat ttgttcggaa taacccatgt
     1321 actcctggac accgaaattg gggacccaca gatcaaagtg cagcgccgca gccatgcaga
     1381 ctggtgacaa atcggaagga ccgtgtgagc cagtacgtac ctgatacagc gaagcaaaat
     1441 cggcaatccg gcgcataccg gtaattccgc ctgcatgggt cagcgtggta cggatataat
     1501 cgataagttg ctcttcaatc agttgcttgc agtcccagat gctgttgaag acttcaccca
     1561 ccgcgatggg tgtgacggta tgttggcgaa tgagacggaa gcattcctgg ttttccgcag
     1621 gcgtcgggtc ttccatccag aacatgcgat aatcttcaat gcttttacca aagcgcgccg
     1681 cttcaatagg cgttaagcga tggtgcatgt catgcagcaa atgttcatta aaaccaaact
     1741 tgttacgtac cgcgtcaaac aatttcggca tgaaatcgag gtatttctcc gtcgaccaca
     1801 gctgctcttc cggccactgt cctttagttg cgggttcata agccagacct ttacctttcg
     1861 acatgccgta ggtggttttc ataccaggga ttccgcactg cacgcggatg gctttgaatc
     1921 ccagctcctg atgacgagca taatcatcca gagcttcatc aatactgtga ccggtggtat
     1981 ggcaataaac catcacccct tcacgagacg cgccgccgag taactggtaa agcggcatgt
     2041 tggcagcttt ggctttaata tcccacagcg ccatatcaac cgctgaaatg gccgacatcg
     2101 tcaccgggcc gcgacgccag taagcacctt tatagaaaaa ctgccagata tcttcgatac
     2161 ggtgtgcatc gcgaccaata agctgcggac aaaggtgatc ctgcaaataa gaggccacgg
     2221 aaagctcacg tccatttagg gtggcatccc caaggcccgt aataccgtcc tcagtggtga
     2281 tttttaatgt gacgaaatta cgccccggac aggtaacaaa aacttcagcc tttacgatct
     2341 tcatgttcga ttccttgcat cgcttgtcgt gatgcatgaa atctacgcaa ctgagctact
     2401 accatacaag tatgaagatc aaaaaaagcc ggagtgatca caaaaaaagg cgtatatttg
     2461 cgccgtgaat ggttgacaaa agatgaaata gaataccttt tgtcagctga cacatcctcg
     2521 tatcttattg ataaaaggga tttatattcc tacgtgcgcc cccagcccgc aacaatgatc
     2581 aacatgccgc aaagcgcaat caacgccccc gtccagtcat aaagactcag tttcacgcca
     2641 tccacaacgc gcagccacat caacgccgtg cagacataaa cgccaccata agcggcgtaa
     2701 acacgcccac tcgccgcagg atgcaacgtt aacaaccaga caaacagcgc cagtgaaatc
     2761 cccgccggaa gcaacagcca gatactggcg tttcgtttta accacaacca gggcagaagg
     2821 catccaataa tttcacacag cgcagtagca aaaaatagta acgttgtttt aatcatcttt
     2881 gtcacttatt gacatcacgt atagttatag ggcgacataa tatcatcaat ataaacaccc
     2941 tccttgaacg ttttgcgttc gcagtggatg gtgtagaatc agctttaatc attcatacag
     3001 ggaatgaatt atgaaaatca ctctcagcaa acgaatcggc ctgctcgctt ttctgctgcc
     3061 ttgcgcactg gcattgagca caactgttca tgccgaaaat aacaaactgg tgattgagtc
     3121 tggcgacagt gcacaaagcc gccagcgcgc cgctatggaa aaagagcaat ggaatgacac
     3181 gcgtaatctg cgccagaaag tgaataaacg cactgaaaaa gagtgggata aagccgacgc
     3241 tgcttttgat aaccgcgata aatgtgagca aagcgccaac atcaatgcct actgggagcc
     3301 aaatactttg cgctgcctgg accgtcgaac tggccgcgtt attaccccct aacctgttat
     3361 tgatttaagg aatgtaagga cacgttatgc caagcgccca cagtgttaag ctacgcccgc
     3421 tggagcgtga agatttacgc tatgtacatc aactcgacaa taacgccagt gtgatgcgtt
     3481 actggtttga ggaaccctac gaagcctttg ttgaactctc tgatctgtac gataagcata
     3541 ttcacgatca gagcgaacgg cgctttgtgg tggaatgtga cggcgaaaaa gccggtctgg
     3601 tggagctggt ggaaattaac catgttcatc gccgcgcaga atttcagata attatctccc
     3661 cggaatatca ggggaaaggt ctggcaacgc gtgccgcaaa attagcaatg gactatggct
     3721 tcaccgttct caatctctat aagctgtatc tgatcgtcga taaagagaat gaaaaagcga
     3781 ttcacattta ccgcaagctt ggctttacgg ttgaaggtga attgatgcat gagttcttta
     3841 ttaatggtca atatcgtaat gccattcgca tgtgcatatt ccagcatcag tatctggcag
     3901 agcacaaaac accgggtcag actctcctga agccgaccgc acaatagcat taataataat
     3961 cgatagtatt tttgatggtg taaacccgtt cgacggcggg ttttactcct tcatcaacaa
     4021 tgattagctg acagtccacc ggattggcgt gactgtcata ttcacagctc tgttttacat
     4081 taccaaccct ttgattattc agtaaagtaa ccgctgtgta atctaatttt ttgatcggat
     4141 ccgttgatgg cgtagcgctg acagataatg ttttgtcgtt acttttcgtg gttttaccca
     4201 gcggataccc ctgatcatca tagcgatatt ccatctgcat ctgtttgctg ctggctttaa
     4261 tcacaaagcc attatcatcg gtttcccagc tcaccccggc agaaggtaat tctgctagct
     4321 ggcattttcc ctgcaaacga actctcttct ccagtgtctg ggcatcacgg taataattgg
     4381 cgtccagtac cagggcaacc acggtattat tttccagatc cagtaattcg agtgaatcaa
     4441 aacagccttc ttccgacaaa gtccccgaaa cacgtttcgt cacttcacct tgctcatcca
     4501 ttaatgtctg agtgaaatct tttaccggac cacgcagcgg atcaaaatcg aattcattag
     4561 agaaactggc catctcaggg gtaaatgaaa gtgttacctc tgtgcggtca catcctgtga
     4621 ggaatatcgc gagtaagcat ggcagtaatt tgtatttcac aacagtcacc agagagtaga
     4681 gatgattctc aatcatagta gcaaatacag tactttacac gttaaatgct atgcttaaag
     4741 aagttatctt cgcgtaagga gcttatgatg aaacgctcaa cctgctgtgc cgcgcttctg
     4801 ctcgccctcg cctcacccgc ggtactcgcc gcgccgggat cctgtgagcg catacagagc
     4861 gatatatcac agcgcattat caataatggc gtacccgaaa gcagcttcac gttaagtatt
     4921 gtacccaatg accaggttga tcagcctgat tcccaggtcg tcgggcattg cgctaatgat
     4981 acgcataaaa ttctctacac ccgcaccacc agcggtaacg tctctgctcc cgcgcagtct
     5041 acccaggatg gcgcgcctgc cgaaccgcag taatacatta tcgactgcac gccggatacg
     5101 acacatccgg cgatttgaat atacaacata atcccacctt attactcata cccttctatt
     5161 gatatggatt aataattctt aacccaaaat aggtagactc cctctattgt tagcgcgcta
     5221 aatattcaat atataaacct ttatataacg ataaagaaca gggagtgagt tatgtccaaa
     5281 aatgaacaaa tggtgggcat cagccgcaga acactcgtta aatctaccgc gataggttct
     5341 ctggcgctgg ctgcaggcgg tttttcttta ccgttcaccc tgcgcagtgc agcggcagcg
     5401 gtacaacaag ccagcgaaaa agtggtctgg ggtgcctgtt ccgtcaactg tggtagccgc
     5461 tgtgcacttc gtctacatgt taaagataat gaagtgacct gggtggaaac tgacaacacc
     5521 ggcagcgatg agtatggcaa tcatcaagta cgcgcctgtt tgcgcggtcg ctccatccgt
     5581 cggcgtatta atcatcccga ccgcttgaat tacccaatga aacgcgtggg caaacgcggc
     5641 gaaggcaaat tcgaacggat tagctgggat gaagccctgg atactatcgc cagtagcctg
     5701 aaaaaaaccg tcgaacaata tggcaatgag gctgtatata ttcagtactc ttcggggatt
     5761 gttggcggca atatgacccg ctcttcgcca tccgcctcgg cggtcaaacg cctgatgaac
     5821 tgctacggcg gttcactcaa ccagtatggc tcctacagca ccgcgcaaat ctcctgtgcc
     5881 atgccctaca cctacggcag taatgatggc aacagcacaa cggatattga aaacagcaag
     5941 ctggtcgtga tgtttggtaa caacccggca gaaacccgca tgagcggtgg tggcatcact
     6001 tatcttcttg aaaaagcgcg cgagaaatcg aacgcaaaaa tgattgttat cgatccgcga
     6061 tataccgata cggctgccgg tcgtgaagac gaatggcttc ctattcgccc gggcaccgat
     6121 gccgcgctgg ttgcgggtat tgcctgggta ttgattaacg aaaatctcgt tgatcaacct
     6181 tttctcgata aatactgcgt cggctatgac gaaaaaacct tacccgcaga tgcaccaaaa
     6241 aatggtcact ataaagccta tattcttggt gaaggagacg acaaaacagc gaaaacgccg
     6301 cagtgggctt cgcaaattac cggtatcccg gtggaccgta tcatcaaact ggcgcgtgaa
     6361 attggcacag caaaacccgc ttatatctgc cagggctggg gaccgcaacg ccaggcaaac
     6421 ggcgagctga ctgcacgcgc tattgcgatg ttgcctattc tgacgggcaa tgtcggcatc
     6481 agcggcggga atagtggcgc gcgtgaatcg acctatacca ttaccataga acgcctgccg
     6541 gtgctggata atccggtcaa aacgtcaatc tcctgcttca gctggacgga tgctatcgat
     6601 catggcccgc aaatgacggc aatccgcgac ggcgtccggg gcaaagataa actggatgtg
     6661 cccattaagt tcatctggaa ctacgcggga aataccctgg ttaatcagca ttctgacatc
     6721 aacaaaacgc atgaaattct gcaggacgaa tcgaaatgcg aaatgattgt ggtcatcgaa
     6781 aactttatga cctcatcggc aaaatatgcc gacattctgc tgccagacct gatgaccgtt
     6841 gagcaggaag atattattcc taacgactac gccggtaaca tgggatatct cattttcctt
     6901 cagccagtca ccagcgaaaa attcgaacgc aaaccgattt actggatcct gagtgaggtc
     6961 gcgaaacgtc ttggacctga cgtctatcaa aagttcacag aaggtcgcac gcaggaacaa
     7021 tggttacaac atctgtacgc caaaatgctt gccaaagatc cggcgttacc gtcttacgac
     7081 gaactgaaaa aaatgggtat ctataagcgt aaagatccca atggtcattt tgtcgcctac
     7141 aaagcatttc gtgacgaccc cgaggcaaat ccacttaaaa cgccttccgg taagattgaa
     7201 atttattcca gcaagctggc ggaaattgcc cgtacctggg aactggaaaa agatgaagtg
     7261 ataagcccac tgccggttta tgcctcaacc tttgaaggct gggactcccc tgaacgtagt
     7321 acttttccac tgcaactgtt tggtttccat tacaaatccc gtactcactc gacctacggc
     7381 aacattgatg tcctgaaggc tgcctgccgt caggaggtgt ggatcaaccc tattgacgca
     7441 cagaaacgtg ggattgccaa cggcgatatg gtgcgggtgt ataaccatcg tggcgaagtt
     7501 cggctaccag ccaaagtgac accacgcatt ctccctgggg ttagcgctat gggccaggga
     7561 gcctggcacg aggccaatat gtctggcgat aaaatcgacc atggcggctg tgtgaatacg
     7621 ctaaccactc tgcgcccttc accactggcg aagggaaacc cgcagcacac taatctggtc
     7681 gagatcgaaa aaatataacc cactacaacc ataaattctg gcatgacatt ttgtttgaaa
     7741 agcaataagt gagtaatgat gaaaatccat accacagagg cgctcatgaa ggctgaaatc
     7801 agccgtagaa gtctgatgaa aacctccgca cttggcagtc tcgcgctggc aagcagtgct
     7861 ttcactctgc cattttccca aatggtccga gcggcagagg caccggtaga agagaaagcg
     7921 gtctggagtt cctgcaccgt taactgcggg agccgctgtc tgttacgttt gcatgtgaaa
     7981 gatgacaccg tgtactgggt ggagtctgat acgacaggtg acgacgtcta cggtaatcat
     8041 caggttcgag cgtgtttacg cgggcgctct atccgccgac ggatgaatca tcctgatagg
     8101 ttgaaatatc ccatgaagcg tgtcggcaag cgcggtgaag gtaaatttga acggataagt
     8161 tgggatgaag ccctggatat catcagtgat aatcttcggc ggatcctgaa agaatacggc
     8221 aatgaggctg tacatgtcct gtacggaaca ggcgtagatg gcggaaacat caccaactcg
     8281 aacgtcccgt accgtctgat gaactcttgc ggtggttttc tcagtcgcta tggcagctac
     8341 agtaccgctc aaatcagtgc cgcaatgagt tatatgttcg gtgccaatga tggcaacagc
     8401 ccggatgata tcgccaatac gaaactggtc gttatgttcg gaaataaccc ggcagaaacg
     8461 cggatgagcg gcggtggtgt cacttactac gtcgagcaag cccgcgaacg ttcaaatgca
     8521 cgcatgatcg tcatcgatcc acgttataac gacacagctg ccggacgtga agatgaatgg
     8581 ctgcccattc gccctggcac cgatggagca ctggcctgtg cgattgcctg ggtactgatt
     8641 actgaaaaca tggtcgatca gccatttctc gacaaatatt gtgttggtta cgatgaaaaa
     8701 acgctgcccg ccaacgcacc acgtaacgcg cattataaag cctatattct gggcgaaggg
     8761 cctgacggca tagctaaaac gccggaatgg gcagcaaaaa tcaccagcat accggcagaa
     8821 aaaattatcc agttggcacg agagatcggt tcagcaaaac ctgcttatat ttgtcagggt
     8881 tgggggccac aacgacactc caacggcgaa caaacgtccc gcgctattgc catgctttcc
     8941 gttctcaccg gtaacgtcgg cataaacggc ggcaactcag gtgtacgcga aggtagctgg
     9001 gatctggggg tagaatggtt cccgatgctt gagaatcctg ttaaaacgca gatttccgtc
     9061 tttacatgga cagatgccat cgaccacggt acggaaatga ccgcgacccg tgatggtgtt
     9121 cgtggaaaag aaaaactgga tgtccccatc aagtttttat ggtgctacgc cagtaacaca
     9181 ttgatcaatc aacatggcga catcaatcac acccatgagg tgcttcagga tgacagcaag
     9241 tgcgagatga ttgttggcat tgaccacttc atgactgcct cggctaagta ttgcgatatc
     9301 ctgttgcccg acctgatgcc aacagagcaa gaagacctta tctctcatga atctgccggg
     9361 aatatgggct atgtgatcct cgcccaaccc gcaacctcag caaaatttga acgcaaaccc
     9421 atctactgga tgctgagcga agttgccaaa cgcttaggac cagacgttta tcaaaccttt
     9481 actgaaggcc gcagtcagca cgaatggatc aaatatctcc atgcgaaaac gaaggaacgt
     9541 aaccctgaga tgcccgacta cgaggagatg aaaacgaccg ggatctttaa gaaaaaatgc
     9601 cctgaagaac actacgtcgc tttccgcgca ttccgtgaag atccacaggc aaacccgttg
     9661 aaaacacctt cggggaaaat cgaaatttat tctgaacgac tggcgacgat tgcggatacc
     9721 tgggaattga aaaaagatga aattattcat ccccttcctg cgtatacccc aggttttgat
     9781 ggctgggacg atcccctgcg gaaaacctat ccactgcagc taacgggctt ccactacaaa
     9841 gcgcgtaccc actccagcta cggcaatatt gatgtgttac agcaggcctg cccacaagag
     9901 gtgtggatca accctattga tgctcaggca cgcggtatcc gtcatggcga taccgtgcgg
     9961 gtatttaaca ataatggaga aatgctgatt gccgcaaaag tgactccgcg tattctgcct
    10021 ggcgttaccg ccatcgggca aggtgcgtgg cttaaagcgg atatgtttgg tgaccgggtc
    10081 gatcacggcg gcagtatcaa tattctgacc tctcaccgcc cttcaccgct ggcaaaggga
    10141 aacccgtcgc acagcaatct tgtccagatc gaaaaggttt aaggagtagc ccatgaccac
    10201 acaatatgga ttttttatcg attccagccg ctgtactggc tgtaaaacct gcgaactggc
    10261 gtgcaaagat tttaaagatc ttggcccgga agtcagtttc cgccgtattt atgaatatgc
    10321 tggcggcgac tggcaggaag ataatggcgt ctggcaccag aacgtgttcg cttattatct
    10381 ctccatctcc tgtaaccatt gcgatgaccc cgcatgtaca aaagtctgcc ccagcggcgc
    10441 aatgcataag cgtgaagatg gctttgtggt ggttgatgaa gatgtttgta tcggctgccg
    10501 ctactgccac atggcttgtc cgtacggcgc gccacagtac aatgctgaaa aaggacacat
    10561 gacgaagtgc gatggttgtt attcgcgcgt cgccgagggg aaacaaccca tatgtgtcga
    10621 atcctgcccg ctgcgtgcac ttgagttcgg tccaattgaa gagctgcgcc agaagcacgg
    10681 cactctggct gccgtcgcgc cactaccgcg cgcgcatttc acaaaaccca atatcgttat
    10741 caaacctaac gccaacagcc gcccgaccgg cgataccact ggttatctgg ctaatccgga
    10801 ggaagtgtaa aatgggaaat ggatggcatg aatggccact ggttatcttt accgtattgg
    10861 gtcaatgcgt agtaggcgcg ctgattgtta gcggaattgg ctggttcgcc gccaataatg
    10921 acgcagaccg tcagcgtatt gtccgtggca tgtttttcct ctggctgttg atgggcatcg
    10981 ggtttattgc ctctgtcatg cacctgggat cgccactgcg agcgtttaac tcgctgaatc
    11041 gaatcggagc ttctggtctg agtaacgaga ttgctgccgg ttccatattt tttgccgttg
    11101 gcggattatg gtggctggtg gcagtcatcg gtaaaatgcc gcaagcgttg gggaaactct
    11161 ggttacttgt cagcatggcg ctcggcgtca ttttcgtctg gatgatgacc tgcgtgtatc
    11221 aaatcgacac agtgccaacc tggtataacg gttacactac gctggctttc ttcctgacag
    11281 tattgttgag cggtccaata cttgctgcca caattctgcg ggcagcccgc gttacattta
    11341 ataccacacc atttgccatc attagcgttc tggcgctgat cgcttgtgcc ggggtgattg
    11401 tgctgcaagg tttgtctctg gcgtctattc actcatccgt acaacaagcc agcgcactgg
    11461 taccagatta cgcctcctta caggtctggc gtgtggtatt gttatgcaca ggtctaggat
    11521 gctggctttg tccgctcatt cgtcgccgtg aacctcacgt tgccggactt attctggggc
    11581 taatcctcat acttggcggt gagatgatcg ggcgtgtgct cttttatggc ctgcacatga
    11641 cggtaggaat ggctattgca ggttaacaaa ttggtgcggg gcaacccgca catttcagga
    11701 tgttaggaat gacccatttt tcacagcaag ataatttttc tgtcgcggcg cgcgtgttgg
    11761 gcgcgctgtt ttattacgct ccagagagtg cggaagccgc acctctggtt gcggtactca
    11821 cccgtgacgg ctgggaaact cagtggcctc taccagaagc gtcattagcg ccgctggtga
    11881 cggcttttca gacacagtgt gaagagactc acgcccaggc ctggcagcgt ttgtttgtcg
    11941 gcccgtgggc actgccgtct ccgccgtggg gatcggtctg gctggatcgc gaatctgtgc
    12001 tgtttggcga ttcaacattg gcacttcgtc agtggatgcg cgagaaaggc attcagtttg
    12061 aaatgaagca aaacgaaccg gaagatcatt ttggttcact gctgttgatg gctgcgtggt
    12121 tggcggagaa tggtcgccag acagaatgcg aagaattgct ggcatggcac ctttttccgt
    12181 ggtcaacacg atttcttgac gtttttatcg aaaaagcaga acaccctttc taccgtgcac
    12241 tgggggaact ggctcgctta acgctggcgc aatggcaatc acaactgttg attcctgtcg
    12301 cggttaaacc actctttcga taggatcacc gtaatattgc cggatggtgg cgattacgcg
    12361 caattcggca ataataggtt acagtgtcac gttttttatc tcttaaagca cgcactgctt
    12421 ttgcggctgg cctcttttgc cgcaaaatag tcgcccgtgt ttcattgccc atttctgctc
    12481 atgcatcatc tacacatcta tccggatctg cgcactatgt tccgccgtct gcttatcgca
    12541 acagtcgtcg gtattcttgc ggcctttgcc gttgccgggt ttcgtcatgc aatgctgcta
    12601 ctggagtggt tgttcctcaa taatgactcc ggcagtctgg tcaatgcagc gacaaatctt
    12661 tccccctggc gacggctgct aactccggcg ctcggcggac tggcggcggg tttgttgctg
    12721 atgggctggc agaaatttac ccagcaacgc cctcatgcgc cgaccgatta catggaagcg
    12781 ttgcaaaccg atgggcagtt tgattacgca gcaagcctgg ttaaatccct tgcctcgctg
    12841 ctggtagtaa ccagcggcag tgcaattggt cgcgaaggtg cgatgattct tttagctgcc
    12901 cttgccgcct cctgttttgc gcaacgtttt acgccacgcc aggagtggaa attatggatc
    12961 gcctgtggag ctgcggcggg aatggctgcg gcctatcgcg ccccgcttgc tggcagttta
    13021 tttatagccg aagtgctgtt tggcactatg atgttggcct ctctcggccc ggtgattatt
    13081 tccgccgtcg tggcgttgct ggttagcaat ctgattaatc atagcgacgc gttactctac
    13141 agcgtacaac tctcagtgac ggttcaggct cgtgactatg cgctgattat cagtacaggt
    13201 gtgctggcag gtctgtgcgg accactgttg ttaacgttga tgaacgcctg tcatcgtgga
    13261 tttgtaagtc tcaaacttgc gccgccctgg caactggcac tgggcgggtt gatcgtgggt
    13321 ctgctttccc tgttcacacc tgcagtgtgg ggcaacggct atagcaccgt acaatccttt
    13381 ttaaccgccc cgccactgtt aatgatcatt gccgggatct tcctctgtaa actgtttgcc
    13441 gtgctggcga gtagtggttc tggcgcgccc ggtggtgtct ttacaccgac gctatttatc
    13501 ggtcttgcca ttggcatgtt gtatggtcgt agcctgggat tatggttccc tgatggcgaa
    13561 gaaattacgc ttttactcgg attgaccggg atggcgacac tgttggcggc aaccacgcac
    13621 gcgccgatta tgtcgacgtt gatgatatgt gaaatgaccg gggagtatca gctactcccc
    13681 ggtttattga ttgcctgcgt aattgcgtcg gtaatttcgc ggacgttaca ccgtgactct
    13741 atctaccgcc agcacactgc gaagcatagc taagcggatg tattgcccca gttcacgctg
    13801 ttcggcgcgc ggcagataag gcagttcacc aatgagcggt gccggaagtt ttttaccgag
    13861 cacatcaatg atttccgcat aatgcgccag tcctggattg attcggttag ccacccagcc
    13921 aatgagcggc agcccgtcgt tggcaatcgc ctgagctgtt agtagtgcat ggttaatgca
    13981 accttcctga ataccgacaa ccatcaatac cggcagttgt tcctgcacca cccattcaga
    14041 gagcggacgc aaatcattca tcagactgcg ccagccgcca gtcccttcta ccacgacatg
    14101 atcgacttta tcggtcaggt ttgccaggcc gtttgaaatg agggtgtaat tgattgggca
    14161 actgtgcgcc acgctacttt cttcttcgct taacgcgata ggattaactg cttcataagg
    14221 cagttcgatg gttgaaacac tctgcaacac cagggcatct ttattacgca gcccttcggg
    14281 tgtctctttg ctccccttcg ctacgggttt atatcccgca accgtttttc cctgggaggc
    14341 taacgcttgt agcaatgcgc gggaaaccac cgttttccct acagaagtgt ctgtaccggt
    14401 aataaagaaa cgcttcagca tcactaactc caccgttatg cttcacaaat ataaaccagg
    14461 aaaataatta accttgaaag tctaagttat gctttcctgg cccaaattga gatagcgcaa
    14521 attttggtag aacagttaaa aaatgttaac cctgcaacag acgaatcaac aaagaaccgt
    14581 tatacatcgc gtcttttacc agcgcagccc ctgccatcgt accctggtta gaaaactgag
    14641 tactctcaac gctaatgtgc tgactatacg caggaagggc ctgctgacgg atgctgtccg
    14701 agatgaccgg gaagaggata tctgccgctt tacttaacgg tgagccaatc agtatttttt
    14761 gtgggttaaa taaattcacc atgatggcaa gaatgcgccc tacatgcgcg cccaccccgg
    14821 taatgatgtc ttttgccagt agatcgccgc gcaatgccgc ctgacacaat gaatccacgg
    14881 ttaacggctg tccatgtaac atcgagctca tggattgatt gagacgcagc tgtgccagct
    14941 caagaatact gtccacactg gcgatggttt cgaggcagcc gtgattcccg caataacagc
    15001 gtttcccata cgggtcgacc tgcgtgtgac ctatttccac gaggctactg ctgcctgcgt
    15061 gtagcagatg accatcggta atgacgcccg cccccacgtt gtgatcgata accacctgaa
    15121 tcacatcgcg cgccccgcgt gaggcaccaa acaaggcctc agccatcgtc catgcgctga
    15181 tatcatgctg aatataaacc ggtacgccgg tatgctgctc cagcgcctcg ccgagcggca
    15241 tctcttttac atcctcgtag aacggcatgc gatgtacaat accattttcc gtatcaataa
    15301 ttcccggcaa ggttatggca atcgaagtca gacgctcaag ttttttctgg tggcggataa
    15361 aaaactgatc gatatgggaa atgatacgat ccagcaatgg cgagtcatct tttaacgcca
    15421 gttcctgcga ctcctccact accagtttgc tgctcagatc gcgcaaagca aggaaaatct
    15481 ccccgcgact aatgcgcaga gaaagatagt gccaggcttc agtttcaacc accagcccca
    15541 ctgccggacg gccacggttc cccgcttctt tgatttccag ctcttgcacc aggtgtgctt
    15601 cgagcatctc acggacaatt ttagtgatac tggcaggagc cagctgcgcc agacgggaaa
    15661 gatcgatacg cgagactgga ccaagctgat caatcaggcg ataaaccgcg cccgcgttgg
    15721 tctgctttat ttgatcaatg tgcccaggct gattttcagc aaccaccgca tactccctat
    15781 attttcgcgc tccgaaataa tctgtaggct atggtgaagc acttcaatac gtgtcgtcaa
    15841 atttttactt aggcatgtga ttaacagcac atttttcggg ctttttcgct gaaatttccc
    15901 tcacctgaga gcattcagca gatgaatacg aaagttacgc gcagccggac tttgttcatg
    15961 gtgtttcggc cagaccaacc acatttcaga aaccgcatcc tcttcagcaa tcggcaccca
    16021 gcgcatttcg ttgagctgaa cacgtttaaa tgacgcaggc aaaattgaaa cacccagacc
    16081 agcggaaacc agaccgatga tggtcattgc ctcacccacc tcctgagtga tgacgggcgt
    16141 caaattgtaa cgtcgcatca gcccgagaat atcgtcatac agccctgtcc ctacgtgcgg
    16201 atcaaaaaag acaaagggtt ctttcgccag ttcagccagc gttacactcg ggttatttgc
    16261 caggggatga tcgtgcggga tcatcgccat aagcggttca tggacgatga ctgcgtgttc
    16321 aagcgtctcc ggtaacgctg tgttacgcag caatcccata tccagcgttc cttcaatgag
    16381 cggagcgatt tgctcgcgag tgttcatttc gcgggtttgt aaatggacat cgggataatc
    16441 acggcgaaac agcgataacg tatcggacac cgcccgaata aaaggagcgg acgaagtaaa
    16501 accaatgcgc aactcccccg cttcgccctg atgcagcctt tcagcgcgag cggcagcatc
    16561 atccaccata gacaggattt gccgactatc tacaagaaac tgttttcctg cttccgtcag
    16621 caatacactg cgattagttc gtgccagcag tcgggcacca atttgttgct ccagcgcctg
    16681 aatctgctga cttagcggcg gttgcgaaat attcaggcgg gcagcggcgc gcccgaaatg
    16741 cagctcttct gcaacagcaa caaagtaacg cagatgacga agttcaatat tcatatttaa
    16801 aacatcttat ttgagattat taatatatta gacagaacaa ttcgattttc ctaatctatt
    16861 cataagcctg atccacaggc atatttagca aggatttcaa gtgagccgta ctacaactgt
    16921 tgatggcgct ccggcaagcg acactgacaa gcaaagcatt tctcagccaa atcaatttat
    16981 taaacgcggt acgccgcaat ttatgcgcgt caccctggcg ctgttctctg ccggactggc
    17041 aaccttcgca cttctctatt gtgtgcaacc tatccttccg gtgctttcgc aggagtttgg
    17101 cttaacgccc gcgaacagta gtatttcact gtccatttcc acggcgatgt tggctatcgg
    17161 tttgctgttt actggcccgt tatccgatgc cattggtcgc aaaccagtga tggtcacggc
    17221 gctactgttg gcctccattt gtacgttact ttcgacaatg atgaccagct ggcacggcat
    17281 tttgattatg cgcgccttga ttgggctttc gttaagtggc gtagcagctg ttggcatgac
    17341 ttatcttagc gaggaaatcc accccagttt cgtggccttt tcgatggggt tgtatatcag
    17401 cggcaactca attggcggca tgagcggacg cttaattagc ggtgtcttca cggacttttt
    17461 caactggcga attgcgctgg cggcaatcgg ttgtttcgcg ctggcctcgg cgttaatgtt
    17521 ctggaaaatc ctccctgaat cacgccattt tcgcccgact tcactgcgcc ctaagacgtt
    17581 attcatcaac tttcgtctgc actggcgtga ccggggatta ccgttattgt tcgcagaagg
    17641 ctttttgctg atggggtcgt tcgtcacgct gtttaattac gtcggctatc ggttgatgct
    17701 ctccccctgg catgtcagcc aggccgtggt tggcttatta tcgctggctt atttgaccgg
    17761 tacatggagc tcacccaaag ccggaaccat gaccacccgc tatgggcgcg gtccggtgat
    17821 gttgttttcg acgggggtta tgctgtttgg tttactgatg accttattta gctcgctgtg
    17881 gctgatcttt gccggaatgt tactcttctc agccggattc ttcgcagccc actcagtagc
    17941 cagcagctgg atcggccccc gcgcaaaacg cgctaaaggc caggcctcct cgctgtatct
    18001 gttcagttac tatctggggt cgagtattgc cgggacgctg ggtggtgttt tctggcataa
    18061 ctatggctgg aacggcgtcg gcgcatttat tgctctgatg ctggtcattg ctctgctggt
    18121 cgggacgcgt ttgcatcatc gtctgcacgc ctgaaaaaat aagtccggac tgaggtaaat
    18181 acccgtccgg acttattgcc tgctcaaacc aacgttaata gccatcctaa aatagacgaa
    18241 gcgccagcca attcccgcag cgcatctagt gtcatcagga ttataagtac ccaaataaac
    18301 ggattcattt tgctgtgtgt catttattac tgatgcgcag ttattctacc gctttgtaag
    18361 tagtaaaata gttaacacga tcaagactac tattattggt agatgaattt tccttaagtc
    18421 acaatacgtt attatcaacg ctgtaattta ttcagcgttt gtacatatcg ttacacgctg
    18481 aaaccaacca ctcacggaag tctgccattc ccagggatat agttatttca acggccccgc
    18541 agtggggtta aatgaaaaaa caaattgagg gtatgacaat gaaaaaagta ttagctctgg
    18601 ttgttgccgc tgctatgggt ctgtcttctg ctgcctttgc tgcagagact gcgaccacac
    18661 ctgctccgac tgcgacgacc accaaagcag cgcctgcgaa aaccacacat cataaaaaac
    18721 agcataaagc agcacctgcc cagaaagcgc aggcggctaa aaagcatcat aaaaatgcca
    18781 aagctgaaca gaaagctcct gaacaaaaag cgcaggcagc gaagaaacac gccaagaaac
    18841 acagccatca gcaaccggca aaacctgctg cacaacccgc agcgtaagtt ttcaacagta
    18901 atgctggcgc gccccctcgc gcctgaaaat tacggtgcta aacgggtaac gtttagcacc
    18961 gcctttagcg gagggtagta tggtgggccg ttatcgcttt gagttcattc ttatcatcct
    19021 tattttatgc gcactgattg ccgcccgttt ttatctttcc tgatcgtagt tatctgattt
    19081 tactcccact ttcatcccgt cccgtctata gtatttatga gggtttgctt ttaataatca
    19141 taattaccca ccagagtgtg atatgcgtag aaccattgct gtagtgttgg gtgcaattag
    19201 tttgacgtct gcttttgtgt ttgcagataa accagacgtt gccaaatcgg caaacgatga
    19261 ggtcagcacc ctgttttttg gtcatgatga tcgtgtgcca gtgaatgaca cgacccaatc
    19321 accgtgggat gcggttggcc aactggaaac ggccagcggc aatttatgta cagcgacgct
    19381 gattgcaccc aatctggcat taacggcagg acactgttta ttgacgccgc caaagggtaa
    19441 agccgataaa gcggtagcgc tgcgtttcgt gtcaaataaa ggtctttggc gctatgagat
    19501 ccacgacata gaaggccgcg ttgatccgac actgggaaag cggttaaaag cagatgggga
    19561 tggttggatt gttcctcccg cagctgcgcc gtgggacttc ggtttgattg tgctacgtaa
    19621 tcccccttct ggcattacac cattgccgtt atttgaggga gataaagcca cgcttaccgc
    19681 cgcattaaaa gcggcaggtc gtaaagtgac tcaggcggga taccctgaag atcatctcga
    19741 tacgttgtac agtcatcaaa actgtgaagt gactggctgg gcgcaaacgt cggtgatgtc
    19801 gcatcagtgc gataccttgc cgggtgacag cggttcgcct ctgatgttgc ataccgatga
    19861 cggctggcaa ttaattgggg tgcaaagttc ggctcctgcc gcgaaagatc gctggcgcgc
    19921 cgataaccgg gccatttctg ttactggttt tcgcgacaag ctggatcaac tgtcgcaaaa
    19981 ataatgttca aacgctgccc gacagcgcgg gcagcgtgtt catcaggcaa gtttcaccat
    20041 gatcattcca gccaacagca gcaccaggcc aatccagcct ttacgattta accgttgacc
    20101 aaacaagatc caacctgcgg ctaacgtggc ggcaatacca aatccgcccc acaatgcata
    20161 agcgacagac aagtcgatcc ctttaacggc ctgagaaagc gcactaaagg cagccagcac
    20221 cgccgccaga gagagcaagc caaatatttt gcgacgaaag ccgtcagaaa atttcaaaaa
    20281 gacgttagca acgatttcca gcacgattgc caatgccagc caggcggcgt gaacccattc
    20341 aaactgcgcc atggttcacc tccagttcag gtttacgcgc tttacgggta cctgatttga
    20401 tcaacacaat cccggcgacc agggtggtta acccggcaat tttcatcaga gataaacttt
    20461 cgtcgaataa caaaacgcta aacaaggtaa taaataaaat accgatacct tcccacagcg
    20521 cataagctac gcctaaggcg atctttttaa cggcgaaaga gagaaatata tacgacagag
    20581 aaatcatcac cagcattaaa ataaagccgc catttccctc actgacgctc gcccatttca
    20641 tggacagcgt accggtaatt tctgtagcaa tagccagacc taataaaatc caataaatat
    20701 acattgccct tctcctgcaa gagaattatt ttaattttcg cttaattcag cgaaaccaaa
    20761 gtaaagtggt caagcccgcg cttagcgcaa gttttggcag aagaaaggac taaagcgcgt
    20821 tgcgccagtg ctgctcacct acgagcaaga tagtggatga ggtacgaaga tgggtaaatg
    20881 tagaaaataa cgtcctgaac aaattgtcca taatattaca attatccgca gtgttgcttc
    20941 tcgtcatcgc ggatgataat tgtcctcggt agttgaacac gcctgatttg tatcatagct
    21001 taagaattaa ctcaaaatat tttcacttct ttacctgagc ggtttgattt tcgttatgat
    21061 gacggagcga aaaagacatt attattagcc aaggaagaaa aaacggggac aagcatggca
    21121 aagccgatca tcacgctcaa tggcctaaaa atcgtcatta tgttgggaat gctggtcatt
    21181 attctctgcg gtatccgttt tgccgccgag atcatcgtgc cgtttattct cgcattattt
    21241 attgctgtta ttcttaaccc gctggtgcaa cacatggtcc gctggcgtgt gccgcgtgta
    21301 ctggcagtgt caattttgat gaccatcatc gtgatggcga tggtgttgct gttagcttat
    21361 ctgggttccg cgctcaatga gttgacgcgg acgttaccgc aatatcgcaa ctctattatg
    21421 acgccactga aagcgattga accgttgttg caacgcgtag ggattgatgt ctcagttgac
    21481 caactggcgc attacattga tccgaacgcg gcgatgacgt tgctcaccaa cttattgacg
    21541 cagttatcta atgccatgtc atcaatcttt ttattgctgc tgacggtgct gtttatgctg
    21601 ctcgaagtgc cacaattgcc cgggaaattt cagcaaatga tggcgcgtcc ggttgaaggg
    21661 atggcggcga ttcagcgtgc gattgacagt gtgtctcatt atctggtgct gaaaacagcc
    21721 atcagcatca tcaccggcct ggtcgcatgg gcgatgctcg ccgcactcga tgttcgcttc
    21781 gcttttgtct ggggattgct ggcctttgcg cttaattaca tcccgaatat tggttcagtt
    21841 ctcgcggcaa tcccccctat tgctcaggta ctggtgttta atggcttcta cgaagcgttg
    21901 ctggtgctgg cgggatatct gctgattaat ctggtcttcg gcaatattct ggagccgcgc
    21961 atcatgggac gtgggctggg gctttcgaca ttggtggtat ttttgtcgtt gattttttgg
    22021 ggatggttgt taggaccggt gggtatgctg ctttccgtgc cgttgacaat tattgtcaaa
    22081 attgcgcttg aacaaacagc gggaggtcaa agcatcgccg ttctgttaag cgatctcaat
    22141 aaagagtgac ggcctccgca gaggccgtca gggttacaga gctttcagga ttgcatccac
    22201 gctggctttg gcgtcaccaa acagcatgtg ggtgttttcc ttgaagaaca gcgggttttg
    22261 cacaccagca tagccagtgt tcatcgaacg tttaaagaca atcacgttct gcgctttcca
    22321 cacttccagc acaggcatac cagcaatcgg actcttcgga tcatcctgcg ccgccgggtt
    22381 aaccgtatcg ttagcaccaa tcaccagtac ggtatcggta tcagcaaagt catcgttgat
    22441 ctcgtccatt tccagcacga tgtcatacgg tacttttgct tcagccagca atacgttcat
    22501 atgtccaggc aaacgccccg caaccgggtg gataccgaaa cgcacgttga taccacgagc
    22561 gcgcagtttc tcggtaattt cagcgacagg atattgcgcc tgcgcgactg ccatgccgta
    22621 ccccggagta atgatcactg aatgggagtt tttcagcagt tccgctgtct cttctgcggt
    22681 gatttcgcgg tgctcgccca cttcctgatc atcgccagta gaagagccgt cggtgccgaa
    22741 accacccgca ataacgctga taaaggaacg gttcatcgcc ttacacataa tgtaagaaag
    22801 gatagcgccc gaagaaccga ccagcgcacc ggtcacaatc agcagatcgt tgctgagcat
    22861 aaagcccgca gccgcagccg cccagccgga gtacgagttc agcatcgaca ccaccactgg
    22921 catatctgca ccaccgatgg aggcgactaa atgccagccg aataccagcg caatggcggt
    22981 cattatcagc aatgccagca cttgcaggcc gacgctgtcc gtgcgaacaa atacaatcag
    23041 cagcaggaag gaaacgacca gagccgccag gttcattttg tgacggtttg gcagcatcaa
    23101 cggtttagac gaaatcttgc cacacagttt gccgaacgcc accaccgaac ccgtgaacgt
    23161 taccgccccg atgaagatac cgaggaacac ttccgtcagg tgaatattga ccagaatcgg
    23221 tgccattccc gcgtcatgat gcagatagct gttaaagcca accagcactg ccgccagacc
    23281 cacgaagctg tgcaggatcg ccaccagttc tggcatttcg gtcatttcaa ctttcttcgc
    23341 cagccggata ccaattgccc caccaatgac catcgccagc aagatccagc caacattacc
    23401 cgtatccggt ccaaaaatgg ttgcgattaa cgcaatcgcc atcccggcga taccgaagtt
    23461 gttaccctgg cgagacgttt catgtttcga aagaccggcc agactgaaga taaacaggat
    23521 cgcggcaaca atgtatgcag ctgtaactaa tcctccagac atatgttacc ccttaatttt
    23581 tgcggaacat tttcagcatg cgctgagtca cggtgaagcc accgaaaata ttaatgctgg
    23641 ctataagcac cgcgataaaa ctaaggaagc taacccagcc gccctggcca atctgcaaca
    23701 gtgctccgac aacaataatc cctgaaatcg cgttggtgac cgacatcaac ggtgtatgca
    23761 gcgcgtgcga tacattccac accacgtaat aaccgacaac acaggccagc gcaaaaacgg
    23821 tgaagtgccc aagaaactct ttcggcgcaa cgcttgccaa ccagccgaaa agaatgattg
    23881 ccagcgccat caacgcgtat ttacgccacg gtgagcaggc acatttttcc tcggttttca
    23941 cttccggtgc cgctttttgt gccgcctgcg gctgagctga tacctgaatc ggcggtgccg
    24001 gccaggtaat ttcgcccgca cggatcacgg tcacgccgcg aatcaccaca tcatcaaaat
    24061 caacagtgat attgccgtct ttctctttgc acaacagttt cagcagatta acgaggttag
    24121 taccgtaaag ctgtgaggat tgcgtcggca gacggcccgg aagatcggta taaccaatca
    24181 ctttgacacc attttccgta gtgaagattt cacccggcac ggtgtattca cagttgccgc
    24241 cgttttgggc tgccaggtcg acaatcacac tacccgcctt catggagtca accatttcac
    24301 gggtaattag cttcggcgct ggtttgcctg gaataagcgc ggtggtgaca atgatatcga
    24361 cctcttttgc ctgggcggca aagagttcca tttccgcttt gatgaacgcg tccgacatca
    24421 ctttggcata gccatcgccg ctgcccgctt cctctttaaa atccagctcg aggaattccg
    24481 cgcccatact ttgaacttgt tctttcactt ccgggcgggt gtcgaatgca cgcacaatcg
    24541 cgccgagact gtttgcagcg ccaatggcgg ccagacccgc aacacccgca ccaatcacca
    24601 tcacttttgc cggtggcact ttcccggccg cagtaatttg cccggtaaag aagcgcccaa
    24661 attcatgtgc cgcttcaaca atggcgcgat aaccggcgat attcgccatc gagcttagtg
    24721 cgtccagcga ttgtgcgcgt gagatacgcg gcacagagtc catcgccatc acggtcacgt
    24781 tacgttccgc aagtttttgc attaattccg gattctgcgc aggccagata aaactcacca
    24841 gcgttgtccc aggattcagt aacgcaattt catcatctaa cggcgcattg accttcagaa
    24901 tgatctctga ctgccagacg ctattccctt ctacaatttc agcgcccgct tgcacaaacg
    24961 ctttatcgtc aaaacttgcc agttgacccg cgccgctctc taccgcgacg gtaaaaccca
    25021 gtttcagcag ctgttccact gtttttggcg ttgctgcaac acgggtttca ttggttaacc
    25081 gttctcttgg tatgccaatt cgcatgatat tcccttccat cggttttatt gatgatggtt
    25141 tgcctgtgtc aggagccaca caagccgctc atgtacgagc taaatgttac tccgttaaaa
    25201 taaattagta acaaacgccc tataacgtac tgaaaattat gcctgtgatc tagcgccaaa
    25261 aatcagtatt tcggcgtgaa ctcgcaaaat attaacgatt cagccgtgat agtgggataa
    25321 acaccttaga acgccggata aagactgata attgtcttcg gcgttcgcat aaaacgagac
    25381 atcgccccgg cacgaatcac tacttaacat taaattaact tatacaattc agttgcttca
    25441 gtagtattga tgctgatacg gctgtttttt aagcatagac ggccatttga gcaggattaa
    25501 aattggctta aggaatgtga tatgaaaaat gacgcagaca gttacaccgt ttaaatgcaa
    25561 taatcagcca cgtttctcgt taataacaat accagtacct ggtttgcgca aggcgaagga
    25621 ttatttttat gaagcttaag aacaccctcc tggcgtcggc actgctttct gctacggcat
    25681 tctccgttaa cgcagcaaca gaactgacac cggagcaagc ggcagcggtt aaaccttttg
    25741 accgtgtagt ggttaccggt cgttttaatg ctattggcga agcggtgaaa gccgtttctc
    25801 gtcgcgctga taaagaaggt gccgcctctt tttatgttgt cgacacttct gattttggta
    25861 acagcggtaa ctggcgtgtg gtcgctgacc tctataaagc cgatgctgaa aaagcagaag
    25921 aaacaagtaa tcgcgtaatt aacggtgttg tcgaactgcc gaaagatcag gctgttctga
    25981 ttgaaccgtt tgacacggtc accgtccagg gcttctatcg tagccagcca gaagtcaatg
    26041 atgccatcac caaagcggca aaagcgaaag gtgcctactc tttctacatc gttcgtcaaa
    26101 tcgatgccaa ccagggcggc aaccagcgta ttactgcatt catctataaa aaagatgcta
    26161 agaaacgtat cgtccagagc ccggatgtga tcccggcaga ttccgaagca ggacgtgcag
    26221 ctctggctgc cggtggcgaa gccgcgaaga aagttgagat cccgggggtt gcgactaccg
    26281 catcaccaag ctctgaagtc ggtcgcttct ttgaaaccca gtcatcaaaa ggcgggcgtt
    26341 acactgtcac gctcccagac ggcactaaag tcgaagaact gaacaaagcg accgcagcga
    26401 tgatggtccc gttcgacagc atcaaattct ctggcaacta cggcaacatg actgaagtct
    26461 cctatcaggt tgcgaaacgt gccgcgaaga aaggtgctaa gtattaccac atcacccgcc
    26521 agtggcaaga acgtggtaat aacctgaccg tcagcgcaga tctgtataaa taactattca
    26581 gttactccta taaggcggct tgatgccgcc tttcgcattt ttagtcacaa atttccccgc
    26641 gaccattgca tgcagacaca tctcttcgta aaatcccgcg cttcagtggc aaactccatt
    26701 tttatgcgtt ttgccctaat atttattctt cttgtcacgt tttaatcact ggatatcgat
    26761 ggaaaagaaa ctgggactga gcgcactcac cgcgctggta ttaagctcaa tgctgggcgc
    26821 gggtgttttc agtctgccgc aaaatatggc ggcagttgcc agcccggcag cactgctcat
    26881 cggctggagt attactggtg ctggtatttt attactggcc tttgccatgc tgatcctcac
    26941 gcgcattcgt cccgaacttg acggcggtat ctttacctat gcccgcgaag gatttggtga
    27001 gctaatcggc ttttgttccg catggggata ctggctgtgc gcggtcatcg ccaacgtctc
    27061 ctatctggtg atcgtttttt ccgcgttaag cttttttacg gacacgccgg aattgcgcct
    27121 gtttggcgat ggtaatacct ggcaatcgat cgtcggtgca tcggctttat tgtggattgt
    27181 tcactttttg atcctgcgcg gagtgcaaac cgctgccagc attaacctgg tggcgacact
    27241 ggcaaaattg ttgccgctgg gtctgtttgt tgtgctggcg atgatgatgt tcaaactgga
    27301 taccttcaag ctcgacttca ccggacttgc acttggcgta cccgtttggg aacaggtgaa
    27361 aaacaccatg ctgatcaccc tgtgggtttt cattggtgtg gaaggtgccg tggttgtttc
    27421 tgcgcgtgcg cgtaataaac gtgatgttgg caaagcgaca ctgctggcgg ttctctccgc
    27481 tctgggcgtt tacttgctgg taacgctgct ttcgctgggc gtggtggcac gtcctgaact
    27541 ggctgaaatt cgtaacccgt caatggccgg actgatggtg gaaatgatgg gaccgtgggg
    27601 cgaaatcatc atcgctgccg gtttgatcgt ttccgtttgc ggtgcgtact taagctggac
    27661 catcatggcg gcagaagttc cgttcctggc agccactcat aaagcattcc cgagcatttt
    27721 tgcgcgccag aacgcacaag ctgcgccatc cgcttcactg tggctgacca atatctgcgt
    27781 gcaaatttgt ctggtactca tctggctgac cggttccgat tacaacacat tgctgaccat
    27841 cgcctcagaa atgattctgg tgccctattt cctggtcggc gcgttcttgc tgaaaatcgc
    27901 aacccgtcca ctgcataaag cggtaggtgt cggtgcctgc atttatggct tatggttatt
    27961 gtacgcctct ggtccaatgc acttgctgtt atccgttgtt ctgtatgcac cggggctgct
    28021 ggttttcctg tacgcgcgta aaacgcatac ccatgataat gtactgaatc gtcaggaaat
    28081 ggtgctgatt ggcatgctgt taattgcctc agttccggcg acctggatgc tggtgggata
    28141 acgttcatcc catcgttgga tagcaaagga gataacgatg ggtaaagccc aacccttgcc
    28201 aatattaatt actggcggag gtcgtcgcat cggcctcgcc ctcgcctggc atttcattaa
    28261 tcaaaagcaa ccggtgatta tcagctatcg gacgcactat cccgccattg atggactgat
    28321 taacgcaggt gcgcagtgta ttcaggctga tttttcgacc aacgacggtg tgatggcgtt
    28381 tgccgatgaa gtactaaaaa gcacccatgg cctgcgtgct attttgcata acgccagcgc
    28441 gtggatggca gaaaaaccag gtacaccact gaccgacgta ctggcttgca tgatgcagat
    28501 ccacgttaat accccatacc tactcaacca tgcactggaa agattactgc gtgggcacgg
    28561 acacgccgcc agcgatatca ttcactttac cgattatgtg gtggagcgcg gtagcgacaa
    28621 acatattgcc tatgctgcaa gcaaagcggc actggataat atgacccgct cgtttgcccg
    28681 caagctggca ccggaagtga aagtgaattc tattgcgcca tcgctaatcc tgtttaatga
    28741 acatgatgat gccgaatatc gacaacaggc gctgaataaa tcactgatga aaaccgcgcc
    28801 tggcgagaaa gaagtaatcg acctggtcga ttacttactt accagctgct ttgtcaccgg
    28861 acgcagtttc cctcttgatg gcggtcgtca tctgcgttaa tgcagcttaa tccagcatat
    28921 aatcaacacc catgcgctta tcccccaaca cgcaacagaa ccaacaaaag cggcgggcaa
    28981 gcgcatcatt ccggtgaaat accacagcga cgccaggtaa acaaaatagg gaataatcga
    29041 ccacatgcta aaaatgatgg ttgcgcgtaa ggcttcaatg ccgcgttcgc tggcaacaat
    29101 ataatgcgcg atgagcgcaa aggtcgggaa aagtgggatc agcccggcga tataataatt
    29161 tttcgttttt gctaaaacac caatcaacag cactaccagc gcaccgagcg cggctttgat
    29221 taccagcccc atctttttac cttaacactt ccataacaag tcaccagtag aatacctgat
    29281 gaaaccttgt ttagaaacga ttgatagtaa gtaaaaacag cgcggtgtat tgtgacgttt
    29341 ttatatctac cgtgaatgtt atgaatacta tcgtatttgt ggaagatgat gcggaagtcg
    29401 gttcactgat tgccgcgtac ctggcaaaac atgatatgca ggttaccgtg gaaccgcgcg
    29461 gcgaccgggc cgaagaaact attctgcgag aaaatccgga tttggtgtta ctcgacatca
    29521 tgctaccagg caaggacggc atgaccattt gtcgtgattt acgcgcaaag tggtccggac
    29581 cgattgttct tctaacctct ctcgatagcg atatgaacca catcctggca ctggaaatgg
    29641 gcgcctgcga ctatattctc aaaacgacgc cccctgctgt tttgctagcg cgtttacgtt
    29701 tgcatttgcg tcagaatgag caagccacag tgaccaaagg tattcaggaa acgtctctga
    29761 ctccctacaa agccctgcat ttcggcacgt tgaccatcga tcccatcaac cgcgtagtca
    29821 ccctggctaa cactgaaatc tcgctctcga cagctgattt cgaattattg tgggaattag
    29881 ctacccatgc cgggcaaatc atggaccgcg atgcattgct gaaaaattta cgcggcgtca
    29941 gttatgacgg actggatcgt agcgtggacg tggctatttc gcggttaaga aaaaaactgc
    30001 tcgataacgc cgcagaacct tatcgcatta aaactgtgcg taacaaaggc tatctttttg
    30061 cgcctcatgc atgggaataa gcgatgaaaa aactgtttat ccagttttac ctgttattgt
    30121 ttgtctgctt ccttgtgatg tctctgctgg ttgggctggt gtacaaattt accgccgaac
    30181 gcgcgggcaa acagtcgctg gatgatttga tgaacagttc gctgtatctg atgcgcagcg
    30241 aattgcgtga gatcccccca cacgattggg gtaagacgct gaaggagatg gatttaaatc
    30301 tctctttcga tctgcgagtc gagccactga gtaaatacca tcttgatgat atttccatgc
    30361 accggctgcg tggcggcgaa attgtcgccc tggacgatca gtacactttt ttgcaacgta
    30421 tcccgcgcag ccactacgtg ctggcagttg gtcctgttcc ttatctttat tacctccatc
    30481 agatgcgatt actggatatc gccctgatcg cttttattgc catttccctc gcctttccgg
    30541 tgtttatttg gatgcgtccg cactggcagg atatgttaaa actggaagca gcggcgcaac
    30601 gatttggcga tgggcatctc aatgaacgta tccactttga tgaaggttcg agctttgaac
    30661 gacttggcgt cgcatttaac cagatggcgg acaatatcaa cgccttgatt gccagcaaaa
    30721 aacagcttat tgacggtatc gctcacgaac tgcgaacacc gttagtgcgc ctgcgttatc
    30781 gactggagat gagcgataac ctgagcgccg tcgaatccca ggcgttgaat cgcgatatca
    30841 gtcaacttga agctttaatt gaagagctgc tgacttatgc ccgactcgat cgcccacaaa
    30901 atgagcttca tcttagcgaa ccagacctgc cgttgtggct gtcaacgcat ctggcagata
    30961 ttcaggcagt aacgcccgat aaaacggtac ggataaaaac gctcgcgcaa ggccattatg
    31021 ctgcgttgga tatgcgctta atggagcgcg tgctggataa tttgctcaat aacgccctgc
    31081 gctactgtca ctcaacggtt gaaaccagcc tgctactgtc ggggaataga gcgacattaa
    31141 ttgttgagga tgatggccca gggattgccc ctgaaaaccg cgaacatatc tttgaacctt
    31201 ttgttcgcct cgatcccagc cgggatcgct caaccggcgg ctgcgggctg gggctggcaa
    31261 ttgtccactc tatagcactg gcaatgggcg gtacggttaa ttgtgacacc agcgaactgg
    31321 gtggtgcccg cttctcgttt agctggccgt tatggcataa catcccgcaa tttacctctg
    31381 cttgacatta cgcgcacggt ggtcaagtca ccaccactgt gctataaaat aaggatgttg
    31441 taactaaagt ggttaatatt atggcgcgtt acgatctcgt agaccgactc aacactacct
    31501 ttcgccagat ggaacaagag ctggctgcat ttgccgctga gctagaacag cacaaattgc
    31561 tggttgcccg cgtgttctct ttgccggagg taaaaaaaga ggatgagcat aatccgctta
    31621 atcgtattga ggtaaagcaa catctcggca acgacgcgca gtcgctggcg ttacgtcatt
    31681 tccgccattt atttattcaa caacagtccg aaaatcgcag cagcaaggcc gctgttcgtc
    31741 tacctggcgt gttgtgttac caggtcgata acctttcgca agcagcgttg gtcagtcata
    31801 ttcagcacat caatcaactc aagactacgt tcgagcatat cgtcacggtt gagtcagaac
    31861 tccccacagc ggcgcgtttt gaatgggtgc atcgtcattt gccggggctg atcaccctta
    31921 atgcttaccg aacgctcact gttctgcacg atcccgccac tttacgtttt ggctgggcca
    31981 ataaacatat cattaagaat ttgcatcgtg atgaagtcct ggcacagctg gaaaaaagcc
    32041 tgaaatcacc gcgcagtgtc gcaccgtgga cgcgcgagga gtggcaaaga aaactggaga
    32101 gagagtatca ggatattgct accctgccac aggacgcgaa gttaaaaatc aaacgtccgg
    32161 tgaaggtgca gccgattgcc cgcgtctggt acaagggaga tcaaaaacaa gtacaacacg
    32221 cttgccctac gccgcttatt gcgctgatta atcgggataa tggcgcgggc gtgccggacg
    32281 ttggggagtt gttaaattac gatgccgaca atgtgcagca ccgttataaa ccacaggcgc
    32341 agccgcttcg tttgatcatt ccacggctgc acctgtatat tgcggattaa cgcccggctt
    32401 tcatactgcc gaccatctgt tctggccgta cccagctgtc aaactcggct tcgctaagat
    32461 accccagcgc aagggctgca gcttttaagg tcagcccttc tttatgcgct tttttggcga
    32521 tctcggcggc tttgtcataa cctatgtggg tgttaagcgc agtcaccagc atcagcgatt
    32581 cattgagtaa ttgattgatt cgcttacgat tcggttcaat gcccactgcg cagtgtttgt
    32641 tgaaactttc catgccatct gccagcaagc gcaccgattg caggaaatta tggatcacca
    32701 tcggacggaa gacgttcagt tcaaagttac cggaagcgcc acccatgttg atcgccacat
    32761 cgttccccat cacctgacag cagagcatgg ttaatgcttc acactgcgtc gggttcactt
    32821 tccccggcat gattgagcta cccggctcat tttccgggat tgagatttca ccaattccgc
    32881 agcgcgggcc agaggccagc cagcggacat cattggcaat tttcatcagt gacgcagcca
    32941 accctttcaa cgcgccgtgc gcctgaacca gggcatcaca ggtcgccagc gcttcaaatt
    33001 tgttcggcgc ggtaacaaac ggtgcacaag taatgactgc cagttcatct gccacgcgac
    33061 gcgcatactc cggatgggta tttagtccag tacccaccgc tgtaccgcca agagccagtt
    33121 ccgctacatg cggcaggctg tattcgatat gtttcagatt atgctcgagc atcgctaccc
    33181 agccggaaat ctcctgcccc agcgttaacg gcgtggcgtc ctgcaagtgg gtacgaccga
    33241 ttttgacgat atcagcaaat acgcgggatt tttcactcag cgtctgggtc agggttttaa
    33301 gttgtggaat gagttgcttg cgcagcgcca gtagtgccgc aacgtgcatc gccgtcggaa
    33361 agacatcgtt ggaactttgg cttttgttca cgtcgtcgtt agggtgaact ttacgttcca
    33421 tcccgcgcac gccaccgagt aattcactgg cccggttagc cagcacttcg ttcatgttca
    33481 tgttactttg cgtgccggag ccggtctgcc agatagccag cgggaattcg tcgtcatgct
    33541 gtcctgccag tacttcatcc gccgcctgcc gaatggcgct cgctttctct tcagacaaca
    33601 agcctaaatc ttcattaact tttgccgctg cgcgcttggt tagtgccagc gcatgaatca
    33661 gtgaggtggg cattttctcc gtcgaaatgc ggaaatgctc cagcgagcgt tgagtttgtg
    33721 cgccccacag cttatctgcc gggacatcaa tcgcccccat cgaatctttt tcgctgcgta
    33781 ctgtattcat ggcctgctcc tcacctgatt aattttttct ttctgttttg ctttcgttaa
    33841 gcaactttta gctcactcat tatttaccat ttgataacaa atgtttggtc tttcgtgcca
    33901 tgtaaaaaaa ccgccccgaa gggcggctct gtttatttca cacagcgggt gcactgtgtg
    33961 agttgtatct gctggaagaa gtcatttcct ttatcatcca caaggataaa cgccgggaaa
    34021 tcttccactt caattttcca gatggcttcc attcctagtt ccggatattc aacacattcc
    34081 aggctcttaa tactaccctg cgccaataca gctgccggac caccgatact accgaggtag
    34141 aaaccgccgt gttttttaca ggcatccgtc acctgctggc tgcggttgcc tttcgccagc
    34201 atgatcatac ttccgccctg cgcttgcagt tgatcgacat aagaatccat ccgtccggcg
    34261 gtcgttgggc caagagaacc ggaggcataa ccttccggcg ttttggccgg acccgcgtag
    34321 taaatcggat gatctttaat atactgcggc accccttcgc cgttatccat ccgctctttc
    34381 agtttggcgt gagcaatatc acgaccaacg ataatcgtgc cattcagcga taagcgtgta
    34441 gaaacgggat actgcgacaa ctgtgcgagg atctctttca tcggacggtt aagatcaacg
    34501 cgcaccgctt cgccttctcc cgctttgcgc agctcttccg gaatatattt gcctggatta
    34561 tgttccagtt tttcgatcca gatcccctga cggttgatct tcgctttgat attacggtca
    34621 gcagaacagg agacgcccat tccgaccggg caggatgcgc cgtgacgtgg caggcgaatc
    34681 acgcggatgt cgtgagcgaa gtatttacca ccaaactgcg cacccagacc aagattttgc
    34741 gcttcgatca gcaattcttt ttccagttcc acatcgcgga acgcctgacc gtgctcattc
    34801 ccttccgttg gcagttcgtc atagtattta gcggaagcca gtttcactgt tttaaggtta
    34861 gtttccgcag aagttccacc aattacgaac gcaatatgat acggaggaca ggccgccgta
    34921 cccagcgtgc gcatcttctc aaccaggtaa tttttcagtt tccccggcgt tagtaacgct
    34981 ttggtttcct gatagagata cgtcttgttt gccgaaccac cacctttggc gatacagagg
    35041 aatttgtact cgtcgccatc aacggcataa agatcgatct gcgctggtag attggtgccg
    35101 gtattcacct ctttatacat atccagcggc gcgttttgcg agtagcgcag attatcttcg
    35161 atataagtgt tatagacacc gcgtgccagc gccgcttcat caccgccacc ggtccataca
    35221 cgctgccctt ttttgccaac aataatcgcg gtgccggtat cctgacaggt tggcagaacg
    35281 cctttcgccg cgatgtcgga gttgcgcagg aattgcagcg ccacatattt atcattttcg
    35341 ctggcttccg ggtcacgcag aatgtcggcc acctgttgtt ggtgagccgg acgcagcata
    35401 aatgacgcat catgaaacgc ctgacgcgcc aacaaagtta acgcttcggg tgcgactttc
    35461 aaaatctcct gcccttcaaa ttcagatacg ctaacgtgtt cgctggttag caggtaatac
    35521 tcagtatcat cttttttgag tggaaaagga gcctgataat gaaagggttt gtttgacatt
    35581 gttctctcac ttactgcctg gtttggttat gctctgggcg ggtgttccgt tgccctgtta
    35641 aaagcgagta acaatatcct acacactttt ttaacaaaaa ctgagactag tacgactttt
    35701 tgcggctcca ggttacttcc cgtaggattc ttgctttaat agcgggatta atttccacat
    35761 taaaacaggg attgatcatg caaaaactca ttaactcagt gcaaaactat gcctggggca
    35821 gcaaaacggc gttgactgaa ctttatggta tggaaaatcc gtccagccag ccgatggccg
    35881 agctgtggat gggcgcacat ccgaaaagca gttcacgagt gcagaatgcc gccggagata
    35941 tcgtttcact gcgtgatgtg attgagagta ataaatcgac tctgctcgga gaggccgttg
    36001 ccaaacgctt tggcgaactg cctttcctgt tcaaagtatt atgcgcagca cagccgctct
    36061 ccattcaggt tcatccaaac aaacgcaatt ctgaaatcgg ttttgccaaa gaaaatgccg
    36121 caggtattcc gatggatgcc gccgagcgta actataaaga tcctaaccac aagccggagc
    36181 tggtttttgc gctgacgcct ttccttgcga tgaacgcgtt tcgtgagttt tccgagattg
    36241 tctccctgct ccagccggtt gcaggtgcac atccggcgat tgctcacttt ttacaacagc
    36301 ctgatgccga acgtttaagc gaactgttcg ccagcctgtt gaatatgcag ggtgaagaaa
    36361 aatcccgcgc gctggcgatt ttaaaatcgg cccttgacag ccagcagggt gaaccgtggc
    36421 aaacgattcg tttaatttct gaattttacc cggatgacag cggtctgttc tccccgctgt
    36481 tgctgaatgt ggtgaaattg aatcctggcg aagcgatgtt cctgttcgct gaaacgccgc
    36541 acgcttacct gcaaggcgtg gcgctggaag tgatggcgaa ctccgataac gtgctgcgtg
    36601 cgggtctgac gcctaaatat attgatattc cggaactggt tgccaatgtg aagttcgaag
    36661 ccaaaccggc taaccagttg ttgacccagc cggtgaaaca aggtgcagaa ctggacttcc
    36721 cgattcctgt ggatgatttt gccttctcgc tgcatgacct tagtgataaa gaaaccacca
    36781 ttagccagca gagtgccgcc attttgttct gcgtcgaagg ccatgcaacg ctgtgtaaag
    36841 gttctcagca gttacagctt aaaccgggtg aatcagcgtt tattgccgcc aacgaatcac
    36901 cggtgactgt caaaggccac ggtcgtttag cgcgtgttta caacaagctg taagagctta
    36961 ctgaaaaaat taacatctct tgctaagctt attaaaggct tataacacct tcaggcggcc
    37021 agtccgcctg atttcatttt atggataatc attatgaata aatcgctggt agcggtaggc
    37081 gtcattgttg cgctaggcgt agtctggaca ggcggcgcat ggtatacagg caagaagatt
    37141 gaaacccatc tcgaagacat ggtcgcgcag gcgaacgcgc aactcaaact gaccgctcct
    37201 gaatccaacc tggaagtcag ttatcaaaac tatcatcgcg gcgtattcag cagccagttg
    37261 caactgttgg tgaaacccat tgccgggaaa gaaaatccgt ggattaaaaa cggtcagagc
    37321 gtcatcttca acgaatcggt tgatcatggt ccctttcccc ttgcccagct caaaaaactg
    37381 aacctgatcc cgtcgatggc atcaattcaa accacgctgg ttaataatga agtaagcaaa
    37441 ccactgtttg atatggcaaa aggtgaaacg ccttttgaga ttaactcccg cattggttac
    37501 agcggtgatt ccagttccga tatttcgctc aagccgctga attacgagca aaaggatgaa
    37561 aaagtcgcct ttagcggcgg cgagttccag ttaaatgcgg acagagacgg caaagctatc
    37621 tccctttccg gggaggcgca aagtggtcgg atagacgcgg ttaacgaata taaccagaaa
    37681 gtacagttga cctttaataa tctgaaaacc gacggttcca gcacgctggc aagttttggt
    37741 gagcgcgtag gaaatcaaaa actgtcactg gaaaaaatga ccatttcagt ggaaggcaaa
    37801 gaactggcac tgctggaagg catggagatc agcggtaaat cggatctggt caatgacggt
    37861 aaaacgatca atagccaact ggattactcg ctaaacagcc tgaaggtaca gaatcaggat
    37921 ctgggcagcg gcaagctgac tttaaaagtc gaccagattg atggtgaagc ctggcatcag
    37981 tttagccagc aatataacgc gcaaactcag gcgctgctgg cacagccaga aattgccaac
    38041 aatcccgaac tttatcagga gaaagtgacg gaagccttct ttagcgctct gccgctgatg
    38101 ctgaaaggcg atccggtgat tactatcgcg ccgctaagct ggaaaaacag tcagggtgaa
    38161 agtgcgctga atctgtcgct gttcctgaaa gatccggcaa cgactaaaga agcgccgcaa
    38221 acgctggcgc aggaagtaga tcgttcggtt aaatctctgg atgcgaaact gaccattccg
    38281 gtggatatgg caactgagtt gatgactcag gtagcgaagc tggaaggtta tcaggaagat
    38341 caagcgaaaa aactggcgaa acagcaagtt gaaggtgcat cagcaatggg gcagatgttc
    38401 cgtctgacca ccttgcagga caataccatc accaccagcc tgcaatatgc taacggtcag
    38461 atcacgttaa acgggcagaa aatgccactg gaagatttcg ttggtatgtt tgcaatgccg
    38521 gcattaaacg ttccggtcgt acccgctatt ccgcagcagt aattcacggc caatacgccg
    38581 gaggtttttc tctccggcgt aatttcttac ttcagcataa agtcatactg aatactcatt
    38641 ttcacatccc agtaattcag gcgatcgccc agcgcactgc catcgcgctt ttcctgccct
    38701 ttatcggcag caagaaagaa attcatccct tgtaacggcc cttcgctgac ccgatagttc
    38761 ataccaccta agatacccgc gccttcaatc cgcacatctc gtttactgga ttcatagccg
    38821 cgggtcacaa tcggtgcaaa tgtcagcgta aattgcggag ttaaacgata gttgacgccc
    38881 aattgccagg actgcattcc ttcacggtta cgatcaagac ttaatgaaaa gggatagcca
    38941 atgtcagtat ccaccacggc accattcatt gcatggcgca aataaccacc actgccaaat
    39001 acgcttcccc acggcgcatt ccaggtcaat tgtccactaa ccaacgccgt ttcattgggc
    39061 tggctggtat tacgacttaa accttcgagc tgcgcgtaaa agccgagcaa ctcccctttc
    39121 aacgtataat tcgggtcgag cggtatgctt ttctcgagaa tactttgtat ctgattggaa
    39181 acatctttct gtaccgatgc ccccagagaa tagcgactgg tatcatcatg ccaactggcg
    39241 gctaaaaaag aacgtgcgcg atgtttatcg tattttcccg tctggctgtt ataaacccct
    39301 tcatcacgaa taccggttgt tgaatcggtc cataaaaata ctcgtgaacg ccacattccc
    39361 cacagatcaa tatttggcgt taaagcccaa taacctaatg ctccttcaga aacagtcggt
    39421 aatgtacgtt gcgaactgcc agccattaac ggaatacgat aaggtaagct accataacta
    39481 taatccgtcc cgaagcgacc taattttagc aggctatttt cactgggttt tatttttact
    39541 gcccccaggg caaagccaaa acttttatca ccatcaagat accaccgggt actcatatca
    39601 gcacgagcac ctaatttata aacataatac gccccgcctt caacgccaat aatattggaa
    39661 taataaccac tattgaaatc gagcaatcct ccctgtaccc atgcgtaaat atctcttccc
    39721 gtgccagcac tgggcttatc agccctgcgt aattcgtttt tcagtctgat gcgtagtccc
    39781 gcttcatcgt cagccggttg cgccgcgtaa gcagaggtaa ggccagaggc agccgtcagg
    39841 caaataacgg ccatggccac tatttttctc atagttgcac ctttgatgtt ct
//
LOCUS       DAPHCV010000036        39311 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.036, whole genome shotgun sequence.
ACCESSION   DAPHCV010000036 DAPHCV010000000
VERSION     DAPHCV010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.036"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..3160
                     /gene="yfaL"
                     /locus_tag="RH033_001860"
     CDS             <1..3160
                     /gene="yfaL"
                     /locus_tag="RH033_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HDV2991056.1"
                     /translation="SDGDGGAIDVTDNSTDNKHLSGKTIINNTSFTNNYAEGYGGAIY
                     TSSTTSPYLIDISVDDNYDQNNGVMIDENNSASGYDHSATAAAGGFMYIGHSVAEFNI
                     AADKTLVIGNTSNDGAIDSLAGTGVIVKEGAGELVLNADNNAFTGEISIQNGEVTLGR
                     SDELMNVGDTHCQSDPQDCFGLMVGSTVHSEYQAELNVGNTQQTFVHSLTGFANGILN
                     IDAGGNVTVNQGGFSGSIQGEGQLTVAQDGSYLLTGAQSMALTGDIVVEDNAVLSLAG
                     NQADLRAMQSDPQSIVLNGGVLDLSDFTTWDGDSSYNDGLQISGSGGTVIGSNDVVDI
                     SSGDDLHIGGSDASQNGVYVVINAGDQRVTLANNNGYLGNTQIASGTLEVSDNSQLGD
                     TSYNRSVIFTDPQQHSEMDVTTDVDTRSATTGQGRNIEMRADGEIHVEDGVDTQWGGL
                     MADSTGQQLESVSTLTKSGGGTLELTASGTATSAVRVEDGTLKGEAENIIPYVSSLWV
                     GEDGVFETGQNQDIRSIDATSGGDIDITDGTVLRLTQQDTNQVLDASLFSGDGTLVNA
                     TDGVTLTGELNTNLETDSLTYLSDVTVNGNLTNTSGAISLQNGVAGDTLTVNGDYTGG
                     GTLLLDSELNGDDSASDQLVLNGNTAGNTAVVINPITGIGEPTSTGIKVVDFAADPTQ
                     FQNNAQFRLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVNPTPPPVPDPTPDPDPTPDP
                     DPTPDPEPTPAYQPVLNAKVGGYFNNLRAANQAFVMERRDHAGGDGQTLNLRVIGGRY
                     HYTAVGQLAQHEDTSTVQLSGDLFSRHWGDDGEWMLGIVGGYSDNQGDSRSNMSGTRA
                     DNQNHGYAVGLTSSWYQHGNQKQGVWLDSWLQYAWFNNDVSEQDDGVDHYHSSGIIAS
                     LEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDDIQTRLGLHSEWR
                     TAVHVIPTLDLNYYHDPHATEIEEDGSTISDDGVKQRGEIKVGVTGNISQRVSLRGSV
                     AWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(3301..4023)
                     /locus_tag="RH033_001861"
     CDS             complement(3301..4023)
                     /locus_tag="RH033_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HDV2991057.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            4170..6797
                     /gene="gyrA"
                     /locus_tag="RH033_001862"
     CDS             4170..6797
                     /gene="gyrA"
                     /locus_tag="RH033_001862"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HDV2991058.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6946..8634
                     /locus_tag="RH033_001863"
     CDS             6946..8634
                     /locus_tag="RH033_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HDV2991059.1"
                     /translation="MSGEKKAKGWRFYGLVGLGAIVLLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKLNSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMSNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGSAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAKIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQALNKTRPAMVDVIPTDGIVPLYINPQGMAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            8631..9254
                     /locus_tag="RH033_001864"
     CDS             8631..9254
                     /locus_tag="RH033_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HDV2991060.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSNQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            9278..13792
                     /locus_tag="RH033_001865"
     CDS             9278..13792
                     /locus_tag="RH033_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HDV2991061.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGKQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     DARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDDSGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTTAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGDLPSGGKSFTVNFDKPGNYNLTLRDEDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARIPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTEKNH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRMGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGDNALMLPEQASNIQLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPDYWQHMLDSYAEQADNMPILHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDTSDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQAAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQNAVLRYGQVEVPLPPGADVERTTWGISVNKPNAGKQQGQL
                     LEKARNEMGKLAYMVPVKELTGTVTFRHLLRFSQKGQFVLSPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            13793..15442
                     /locus_tag="RH033_001866"
     CDS             13793..15442
                     /locus_tag="RH033_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HDV2991062.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDTLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVNSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVLVELFARYPLKKITAEKSS
                     TAVKPGVLNGRYRVTFANGNHIMFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            15447..16223
                     /locus_tag="RH033_001867"
     CDS             15447..16223
                     /locus_tag="RH033_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HDV2991063.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPIHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(16297..17481)
                     /gene="atoB"
                     /locus_tag="RH033_001868"
     CDS             complement(16297..17481)
                     /gene="atoB"
                     /locus_tag="RH033_001868"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HDV2991064.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHVDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTLVFSQDE
                     FPKADSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLNPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     PEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(17512..18834)
                     /locus_tag="RH033_001869"
     CDS             complement(17512..18834)
                     /locus_tag="RH033_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HDV2991065.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVMPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(18831..19481)
                     /locus_tag="RH033_001870"
     CDS             complement(18831..19481)
                     /locus_tag="RH033_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-CoA--3-ketoacid-CoA transferase"
                     /protein_id="HDV2991066.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGILPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(19481..20143)
                     /gene="atoD"
                     /locus_tag="RH033_001871"
     CDS             complement(19481..20143)
                     /gene="atoD"
                     /locus_tag="RH033_001871"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HDV2991067.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYHLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(20339..21724)
                     /gene="atoC"
                     /locus_tag="RH033_001872"
     CDS             complement(20339..21724)
                     /gene="atoC"
                     /locus_tag="RH033_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HDV2991068.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSNETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEAKTAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(21721..23547)
                     /gene="atoS"
                     /locus_tag="RH033_001873"
     CDS             complement(21721..23547)
                     /gene="atoS"
                     /locus_tag="RH033_001873"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HDV2991069.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGNRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKTLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMI
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDHTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGDMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTGDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARIDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIRTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            23714..26563
                     /gene="rcsC"
                     /locus_tag="RH033_001874"
     CDS             23714..26563
                     /gene="rcsC"
                     /locus_tag="RH033_001874"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HDV2991070.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQTDVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPLTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(26763..27413)
                     /gene="rcsB"
                     /locus_tag="RH033_001875"
     CDS             complement(26763..27413)
                     /gene="rcsB"
                     /locus_tag="RH033_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HDV2991071.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(27430..30102)
                     /gene="rcsD"
                     /locus_tag="RH033_001876"
     CDS             complement(27430..30102)
                     /gene="rcsD"
                     /locus_tag="RH033_001876"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HDV2991072.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTTPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPEGKQLTNQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            30842..31969
                     /gene="ompC"
                     /locus_tag="RH033_001877"
     CDS             30842..31969
                     /gene="ompC"
                     /locus_tag="RH033_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HDV2991073.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNAPESENNSWTRVAFAGLK
                     FQDIGSIDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGQNGSVSGESDPDFTGHGITNNGRKALRQNGDGVGGSITYDYEGFGI
                     GGAISSSKRTWDQNNTGLIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLG
                     WANKAQNFEAVAQYQFDFGLRPSVAYLQSKGKNLGTIGTRNYDDEDILKYVDVGATYY
                     FNKNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            32081..33136
                     /gene="apbE"
                     /locus_tag="RH033_001878"
     CDS             32081..33136
                     /gene="apbE"
                     /locus_tag="RH033_001878"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HDV2991074.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGAGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            33210..34274
                     /gene="ada"
                     /locus_tag="RH033_001879"
     CDS             33210..34274
                     /gene="ada"
                     /locus_tag="RH033_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HDV2991075.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRNTGIFCRPSCRA
                     RHALRENVSFYADASEALAAGFRPCKRCQPDKANPQQHRLDKITHACRLLEQETPVTL
                     ESLAEQLAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTQAILSAGFP
                     DGSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADSAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            34274..34924
                     /gene="alkB"
                     /locus_tag="RH033_001880"
     CDS             34274..34924
                     /gene="alkB"
                     /locus_tag="RH033_001880"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HDV2991076.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHHLCQR
                     AATAAGYPDFQPDACLINRYVPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKVGVHPLTTDCRYNLTFRQAGKKE"
     gene            35000..36643
                     /gene="yojI"
                     /locus_tag="RH033_001881"
     CDS             35000..36643
                     /gene="yojI"
                     /locus_tag="RH033_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HDV2991077.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRVLDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLVQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            36861..38507
                     /gene="mqo"
                     /locus_tag="RH033_001882"
     CDS             36861..38507
                     /gene="mqo"
                     /locus_tag="RH033_001882"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HDV2991078.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNSWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(38657..39145)
                     /gene="eco"
                     /locus_tag="RH033_001883"
     CDS             complement(38657..39145)
                     /gene="eco"
                     /locus_tag="RH033_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HDV2991079.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
BASE COUNT         9589 a         9694 c        10576 g         9452 t
ORIGIN      
        1 tagtgatggg gacggcggtg ccattgatgt tacggataat agtacggata acaaacatct
       61 ttcaggaaaa acgataatta ataatacctc ctttaccaat aactatgcag aaggttatgg
      121 cggagcaatt tataccagca gcaccacatc cccgtatctt attgatattt ctgttgatga
      181 taattatgac caaaacaatg gtgtgatgat tgatgaaaat aacagcgcct cgggatatga
      241 ccattcggcg acggcagcgg cggggggctt tatgtatatc ggtcatagtg tggctgaatt
      301 taacattgcc gccgataaaa cgctggtaat aggcaatacc agcaacgatg gcgcgattga
      361 ttctcttgcc ggaacgggag tcatcgttaa agagggagcg ggagagttag tcctcaatgc
      421 cgacaataat gcgtttaccg gcgagataag tatccagaat ggcgaggtga cgttggggcg
      481 cagcgatgag ttaatgaatg tcggcgacac gcactgtcag agcgatccgc aggattgctt
      541 tggtctgatg gtcggcagca ctgttcattc tgagtatcag gcagaactga atgttggcaa
      601 tacacaacaa acgtttgtgc actcattaac cggttttgct aatggcattt tgaatatcga
      661 cgcaggcggc aatgttaccg taaaccaggg cggtttttcc ggctcgattc agggtgaagg
      721 acagttgacg gtagcgcaag atggcagcta tctcctgaca ggggcgcagt cgatggcatt
      781 aaccggcgat attgtggtgg aagataacgc cgtattgtcg ctagcgggga atcaggccga
      841 tttacgggca atgcagagcg atccgcaatc gatcgtgcta aatggcggag tattggatct
      901 ctctgatttc accacctggg atggcgatag ctcatacaat gacggtctgc aaatcagcgg
      961 cagcgggggg acagtgattg gcagtaatga tgtcgttgat atcagcagtg gcgatgattt
     1021 acatattggc ggcagtgatg ccagccagaa tggcgtttat gtcgtcatta atgcggggga
     1081 tcagcgcgta acgctggcga ataacaacgg ttatctcggt aatacgcaaa tcgcctccgg
     1141 tacgcttgaa gtcagcgata attcgcaatt gggggacaca agttacaacc gttcagtgat
     1201 cttcacagat ccccaacaac acagtgaaat ggatgtgacg actgatgttg acactcgctc
     1261 ggctacaaca ggtcagggca ggaatattga aatgcgcgct gatggcgaaa tacacgttga
     1321 ggatggcgta gatacgcaat ggggcgggtt gatggcggat agtaccgggc aacaactgga
     1381 gagcgtaagc acgttgacta aaagtggcgg cggtacgctg gagttgaccg ccagcggcac
     1441 tgcgacgtct gcggtacgtg tggaggacgg aacgctaaaa ggtgaagcgg agaatattat
     1501 tccttatgtt tcttcactgt gggtggggga agacggtgtt tttgaaaccg ggcaaaatca
     1561 ggatattcgc tcaatcgatg ccacctctgg cggcgatatc gacataaccg atggtactgt
     1621 gttgcgattg acgcagcagg atacgaacca ggtgctggat gcctcgctgt ttagcggcga
     1681 cggtacgttg gtgaatgcca ccgatggcgt gacgctgaca ggcgagctta ataccaacct
     1741 tgaaactgac agcctgactt atctttccga cgtgacggtt aatggcaatc tgaccaatac
     1801 gtccggtgcg attagcctgc aaaatggcgt cgctggcgac acgctgacgg taaatggtga
     1861 ttataccggc ggcggtacgc tactgctcga cagcgaatta aacggcgatg actcggcaag
     1921 tgaccaactg gtattgaacg gtaatactgc tggcaacacg gccgtggtaa ttaaccccat
     1981 tacgggtatt ggtgagccga catctacagg tattaaagtg gttgatttcg cagctgatcc
     2041 cacgcagttt caaaacaatg cgcagttccg tctggcagga agcggctacg tcaatatggg
     2101 ggcgtatgat tacacgctgg tggaagataa caacgactgg tatctgcgat cacaagaagt
     2161 caatccgacg ccgccgcctg ttccagaccc aactccagat cctgatccca cgccggatcc
     2221 cgacccaacc cccgaccctg aacctacgcc tgcttaccag ccggtgttga atgccaaagt
     2281 tggtggatat tttaataacc tacgtgcggc aaatcaggca tttgtgatgg agcgacgcga
     2341 tcacgcaggt ggcgatggtc agacgctgaa tttacgcgtt atcggcgggc gttatcatta
     2401 cacggcggta gggcagttgg ctcagcatga agacacttct acggtgcagc ttagcggcga
     2461 tctgtttagc aggcactggg gtgatgatgg cgagtggatg cttgggattg ttggtggcta
     2521 cagcgataac cagggcgaca gccgctcgaa tatgagcgga actcgcgccg ataaccagaa
     2581 ccacggttat gccgttgggc tgacctcaag ctggtatcag cacggtaatc agaagcaggg
     2641 ggtctggctg gatagctggc tgcaatatgc gtggtttaac aatgatgttt ctgaacaaga
     2701 cgatggcgtg gatcattacc actcgtcggg gattatcgcc tcgctggaag ccgggtatca
     2761 atggttaccg gggcgtggtg tggtgattga accgcaggcg caggtgattt atcagggcgt
     2821 gcagcaggat gattttaccg ccgctaacca tgcgcgcgtg tcacaatcgc agggtgatga
     2881 tattcagacg cggctgggtt tacacagcga atggcgtacc gctgttcatg tcataccaac
     2941 attagatctg aattattatc acgatcccca tgcgacggaa attgaagaag atggcagcac
     3001 tatcagtgac gatggggtga agcaacgggg tgaaataaaa gtgggagtca cgggcaatat
     3061 cagtcagcga gtgtccctgc gcggcagcgt ggcgtggcag aaagggagtg atgattttgc
     3121 ccagacggca gggtttttgt cgatgacggt gaaatggtaa tcgctgtctg accatcacta
     3181 ttgccggatg cggcgtaaac gccttatctg gcctacggag agcatttgta ggcctgataa
     3241 gacgcggcaa gcgtcgcatc aggcattggc gcggttgccg gatgaatcca aacaacaacc
     3301 tcacttattc tgcgtgtgca gcatatagtt cacatccacg ccggggccga gcttaaagga
     3361 attagtgatc gggttgtaat gtaacccggt tatatgtcgc tctttaaggc ttgtctgatc
     3421 tacccagccc agcagctctg ccggtttaat aaacttcttc acatcatgcg tgcctttggg
     3481 caccatgcgc aaaatatatt ccgcgccaac caccgccatc agccatgact tgccgttgcg
     3541 gttaagcgtc gagaaaaaga catcgccgcc cggtttcacc agttgcgcac aggctctgac
     3601 cactgactgc ggatcgggga cgtgctccag catctccatg caggtcacca catcatactg
     3661 cccggcatgt tttgccgcgt gctcttccac ggtttcctgc acgtaatcca cctgaatgcc
     3721 gctttccagc gcgtgcagct ttgccacctg caatggctca aagcccatat ccagaccggt
     3781 caccgtcgca ccttcgcgcg ccatactctc ggccagaatg ccgccgccac aaccgacatc
     3841 gagcaccttt ttgccaaata aaccgccagc acgctcggca atatagccca gacgcagcgg
     3901 gttaatgcgg tgcagcggtt tgaactcacc ttcaagatcc caccagcggg aggcgacagc
     3961 ttcaaattta gcgatctctt cgtggtctac gttatggttt accggcgatt tttcggcatt
     4021 cattggcact tctactccgt aattggcaag acaaacgagt atatcaggca ttggaggtga
     4081 ataaagcgta taggtttacc tcaaactgcg cggctgtgtt ataatttgcg acctttgaat
     4141 ccgggataca gtagagggat agcggttaga tgagcgacct tgcgagagaa attacaccgg
     4201 tcaacattga ggaagagctg aagagctcct atctggatta tgcgatgtcg gtcattgttg
     4261 gccgtgcgct gccagatgtc cgagatggcc tgaagccggt acaccgtcgc gtactttacg
     4321 ccatgaacgt actaggcaat gactggaaca aagcctataa aaaatctgcc cgtgtcgttg
     4381 gtgacgtaat cggtaaatac catccccatg gtgactcggc ggtttatgac acgatcgtcc
     4441 gtatggcgca gccattctcg ctgcgttaca tgctggtaga cggtcagggt aacttcggtt
     4501 ccatcgacgg cgactctgcg gcggcaatgc gttatacgga aatccgtctg gcgaaaattg
     4561 cccatgaact gatggccgat ctcgaaaaag agacggtcga tttcgtagat aactatgacg
     4621 gcacggaaaa aattcccgac gtcatgccaa ccaaaattcc taacctgctg gtgaacggtt
     4681 cttccggtat cgccgtaggt atggcaacca acatcccgcc gcacaacctg acggaagtca
     4741 tcaacggttg tctggcgtat atcgatgatg aagacatcag cattgaaggg ctgatggaac
     4801 acatccccgg gccggacttc ccgacggcgg caatcattaa cggtcgtcgc ggtattgaag
     4861 aagcttaccg taccggtcgc ggcaaggtat atatccgcgc ccgtgctgaa gtggaagttg
     4921 acgccaaaac cggacgtgaa accattatcg tccacgaaat tccgtatcag gtaaacaaag
     4981 cgcgcctgat cgagaagatt gcggaactgg taaaagaaaa acgcgtggaa ggcatcagcg
     5041 cgctgcgtga cgagtctgac aaagacggta tgcgcatcgt gattgaagtg aaacgcgatg
     5101 cggtcggtga agttgtgctc aacaacctct actcccagac ccagttgcag gtttctttcg
     5161 gtatcaacat ggtggcattg caccatggtc agccgaagat catgaacctg aaagacatca
     5221 tcgcggcgtt tgttcgtcac cgccgcgaag tggtgactcg tcgtactatt ttcgaactgc
     5281 gtaaagctcg cgatcgtgct catatccttg aagcattagc cgtagcgctg gcgaacatcg
     5341 acccgatcat cgaactgatc cgtcatgcgc cgacgcctgc agaagcgaaa actgcgctgg
     5401 tggctaatcc gtggcagctg ggcaacgttg ccgccatgct cgaacgtgcg ggcgatgatg
     5461 ctgcgcgtcc ggaatggctg gagccagagt tcggcgtgcg tgatggtctg tactacctga
     5521 ccgaacagca agctcaggcg attctggatc tgcgtttgca gaaactgacc ggccttgagc
     5581 atgaaaaact gctcgacgaa tacaaagagc tgctggatca gatcgcagaa ctgctgcgta
     5641 ttcttggcag tgccgatcgt ctgatggaag tgatccgcga agagctggaa ctggttcgtg
     5701 aacagttcgg tgacaaacgt cgtactgaaa tcaccgctaa cagcgccgac atcaacctgg
     5761 aagatctgat cacccaggaa gatgtggtgg tcacgctttc tcaccagggt tacgtgaagt
     5821 atcagccgct ttctgaatat gaagcgcagc gtcgtggcgg gaaaggtaaa tctgccgcac
     5881 gtattaaaga agaagacttt atcgaccgac tgctggtggc gaacacccac gaccatattc
     5941 tgtgcttctc cagccgtggc cgcgtctatt cgatgaaggt ctaccagtta ccagaagcca
     6001 ctcgtggcgc gcgtggtcgt ccgatcgtca acctgctgcc gctggagcag gacgaacgta
     6061 tcactgcgat cctgccggtg accgagtttg aagaaggcgt gaaagtcttc atggcgaccg
     6121 ctaacggtac tgtgaagaaa accgtcctca ccgagttcaa ccgtctgcgt accgccggta
     6181 aagtggcgat caaactggtt gaaggcgatg agctgatcgg cgttgacctg accagcggcg
     6241 aagacgaagt aatgctgttc tccgccgaag gtaaagtggt gcgctttaaa gagtcttctg
     6301 tccgtgcgat gggctgcaac accaccggtg ttcgcggtat tcgtttaggc gaaggcgata
     6361 aagtcgtctc tctgatcgtg cctcgtggcg atggcgcaat cctcaccgca acgcagaacg
     6421 gttatggtaa acgtaccgca gtggcggaat acccaaccaa gtcgcgtgcg acgaaagggg
     6481 ttatctccat caaggttacc gaacgtaacg gtttagtggt tggcgcggtg caggtagatg
     6541 actgcgacca gatcatgatg atcaccgatg ccggtacgct ggtacgtact cgcgtttcgg
     6601 aaatcagcat cgtgggccgt aacacccagg gcgtgatcct catccgtact gcggaagatg
     6661 aaaacgtagt gggtctgcaa cgtgttgctg aaccggttga cgaggaagat ctggatacca
     6721 tcgacggcag tgccgcggaa ggggacgatg aaatcgctcc ggaagtggac gttgacgacg
     6781 agccagaaga agaataattt tacttcttca tgccaaaagg gagctatctc ccttgtttga
     6841 attgaaaagt ccaggctgca aagtctgggc ttttgtcgta ttagggcgcg gtaaagtttt
     6901 gctgtgctcg taaaaaatgg ctggctttac acaaggaatg tggcaatgag tggtgaaaaa
     6961 aaggcgaaag gctggcggtt ctatggtctt gtaggtttgg gcgcaatagt actgctttcc
     7021 gctggcgtct gggcgttgca atatgctggc agtgggccag aaaaaacgtt gtcgccgctg
     7081 gtggtgcaca acaatctgca aatcgatctc aatgagccgg acctctttct cgacagcgac
     7141 tctctgagcc agcttcccaa agacctcctt accattccgt ttctccacga tgttctgagt
     7201 gaagatttcg ttttctatta tcagaatcat gccgatcgtc tgggcattga aggcagcatt
     7261 cgccgcattg tctatgaaca cgatctcacg ctgaaagata agctcttttc gtcactctta
     7321 gatcagcccg cgcaggcggc gctatggcac gataaacaag gccatctttc acattacatg
     7381 gtgctgatcc agcgcagtgg tttaagcaaa ctgttggagc cattgttgtt tgccgctacc
     7441 agcgacagcc agttaagcaa aacggaaatc agtagtatta agctaaatag tgaaaccatc
     7501 ccggtttatc agttgcgcta taacggcaat aacgccctga tgttcgcgac ttatcaggac
     7561 aagatgctgg tgttttccag tacggatatg ctgtttaaag atgaccagca ggataccgaa
     7621 gccacggcga tcgcaagtga tttgttgagc ggtaaaaaac gctggcaagc cagctttggc
     7681 ctggaagagc gtgctgctga aaaaacgcca gtacgccagc gtattgttgt cagcgccagg
     7741 ttgctggggt ttggctacca gcgattaatg ccttcttttg ctggcgtacg cttcgaaatg
     7801 agcaacgacg gctggcatag ctttgtggcg ttaaatgatg aatccgccag cgtagatgcc
     7861 agtttcgatt ttacgccggt atggaacagt atgcctgccg gggccagctt ctgtgtggcg
     7921 gtgccgtatt cgcatggtat tgccgaagag atgctttcgc acatcagcca ggaaaacgac
     7981 aagctgaacg gggcgttaga tggtgcagcc gggctgtgct ggtatgaaga ctcaaaatta
     8041 caaaccccgc tatttgttgg tcaatttgat ggctcagccg aacaggcaca actgcctggg
     8101 aaactgttta cgcaaaatat tggtgcgcac gaaagcaaag cgccagaagg tgttttgccg
     8161 gtaagccaga ctcagcaggg cgaagcgaaa atctggcgtc gcgaagtgag ttcccgatac
     8221 ggtcagtatc cgaaagcgca ggcggcgcaa cccgatcaat taatgtcgga ttattttttc
     8281 cgcgtgtcgc tggcgatgca aaacaaaacg ctgcttttct ccctcgatga cacgctggtt
     8341 aataacgccc ttcaagcact gaataaaacc cgcccggcaa tggtggatgt aatacccact
     8401 gatggcatcg ttccgctcta tatcaatcca caaggaatgg cgaaactgct gcgtaacgaa
     8461 acgctgacca gtctgccgaa gaatctcgaa ccggtttttt ataacgccgc acaaacttta
     8521 ttaatgccga agctggacgc tttatctcaa caaccgcgtt atgtcatgaa gctggcccag
     8581 atggaacccg gtgccgcctg gcagtggcta cccataacct ggcaaccgct atgagacacg
     8641 ggctgctggc gctgatttgc tggctgtgtt gtgttgttgc ccatagcgaa atgctgaatg
     8701 tcgaacaatc cgggctgttt cgcgcctggt ttgtacgcat tgcacaggaa cagctccgcc
     8761 agggtccaag tccacgctgg tatcagcagg attgtgcggg cctggtgcga tttgcagcga
     8821 acgagacgct gaaagttcac gacagtaaat ggctaaaaag taacgggtta tctaaccagt
     8881 atttgccgcc agagatgacg ctaacacctg aacagcggca actggcgcaa aactggaatc
     8941 aggggaacgg gaaaaccggc ccctacgtga ccgcaattaa tttgattcag tacaacagcc
     9001 aatttattgg ccaggacata aaccaggcgc tgcctggcga tatgattttt ttcgatcagg
     9061 gcgatgccca gcacttaatg gtctggatgg ggcgttacgt catctaccac accggaagcg
     9121 ccacgaaaac tgacaacgga atgcgcgcag tcagtctgca acaacttatg acatggaagg
     9181 acacccgatg gatacccaac gattccaatc ccaatttcat tggcatttat cgtttaaatt
     9241 ttctggcgcg atagcagcgt gcttgtccct gtctcttgtg ggaacagggc ttgccaatgc
     9301 tgatgattcg cttccttcca gcaactatgc gccgcccgcc gggggaacat tctttttgct
     9361 cgctgacagc agttttagca gcagtgaaga ggcgaaagtg cgactggaag cgccggggcg
     9421 tgattatcga cgctatcaga tggaagagta cggcggcgtg gacgttcgcc tgtatcgtat
     9481 tcctgacccg atggcatttt tgcgccagca gaaaaacctg catcgcattg tggtgcaacc
     9541 gcaatatttg ggcgacgggc tgaacaatac gctgacctgg ctgtgggata actggtacgg
     9601 caaatctcgc cgcgtgatgc agcgtacttt ttcttctcag tctcggcaga atgtgactca
     9661 ggcgttaccc gaattacagc tcggtaatgc cattattaaa ccttcccgtt atgtacagaa
     9721 caaccagttt tcaccgctga aaaaatatcc cctggtggaa cagttccgtt acccattatg
     9781 gcaggctaaa ccggtcgagc cgcagcaagg ggtaaaactg gaaggcgcat ccagcaattt
     9841 catctcgccg cagccgggca acatctatat tccgctaggc aagcaagagc cggggctgta
     9901 cctcgtcgag gcgatggtag gtgggtatcg ggcgacgacg gtggtgtttg tttccgatac
     9961 cgtggcgctt agcaaagtgt caggtaacga gcttctggtg tggaccgcgg gtaaaaaaca
    10021 gggggaagcg aagccgggat cagagatctt gtggactgac ggtcttggcg tgatgacccg
    10081 cggtgtgacc gatgacagcg gtaccttgca gttacaacat atatcgccgg aacgttcata
    10141 cattctgggt aaagatgctg aaggcggcgt ttttgtctcc gagaattttt tctacgaaag
    10201 cgaaatctac aacacccgct tgtatatctt taccgaccgc cctctgtatc gtgcaggcga
    10261 tcgtgttgat gtcaaagtga tgggccgcga gttccacgac ccgttgcatt catcccccat
    10321 cgtcagcgcc ccggcgaagc tttcggtgct ggacgctaac ggcagtctgt tgcaaaccgt
    10381 caatgtcacg ctggatgcgc gcaatggcgg gcaggggagt ttccgcctgc cagaaaatgc
    10441 cgttgccgga ggttatgagt tacgcctggc ttaccgcaat caggtctata gcagtagttt
    10501 tcgcgtagcc aactatatca agccacattt cgagattggt ttagctctcg acaaaaaaga
    10561 gttcaaaact ggcgaagcgg tcagcggcaa actgcaactg ctttacccgg atggtgagcc
    10621 ggtaaaagat gcccgcgtgc agttaagttt gcgcgctcag caattatcaa tggtcggtaa
    10681 cgatttgcgt tatgccggac gttttcccgt gtcgctggaa ggcagcgaaa cggtgtccga
    10741 tgatagcggt catgtggctt taaatctccc cgccgccgat aaaccgagcc gctatttgtt
    10801 aaccgtctcc gccagtgacg gcgcggcgta tcgcgtcacc accaccaaag agatcctcat
    10861 tgaacgcggc ctggcgcatt actctttaag taccaccgca caatacagta atagcggcga
    10921 gtcggttgtg ttccgttatg ccgcgctgga atcttcaaaa caggttcctg ttacgtatga
    10981 atggttgcgt ctcgaagacc gcacgagcca tagcggagat ctaccgtcag gcggcaaatc
    11041 ctttaccgtc aatttcgata aacctggcaa ctacaatctg acattacgcg atgaagacgg
    11101 cttaattctc gccgggttaa gccatgcagt cagcggtaag ggcagcacgg cgcatacggg
    11161 tacggtagat atcgtggcgg ataaaacgct gtaccagcca ggcgaaaccg cgaagatgct
    11221 gattaccttc ccggagccaa ttgatgaagc attattgacg ctggaacgcg atcgcgttga
    11281 acagcagtcg ctgctatcgc atccggcaaa ctggctgacg ttacaacgtt taaacgatac
    11341 ccagtatgaa gcccgcatac cagtgagcaa ttcctttgcg cctaacatca ctttttcggt
    11401 gctgtatacc cgtaacggtc agtacagttt tcagaacgcc gggatcaaag ttgccgttcc
    11461 ccagctggat atccgggtga aaacggaaaa aaaccattac cagcctggtg aactggtcaa
    11521 tgtcgaatta acctcgtcgc tgaaaggtaa acctgtttct gcgcagctaa cggttggtgt
    11581 ggtcgatgaa atgatctacg cgctgcaacc ggaaatcgcg ccgaatatcg gcaaattttt
    11641 ctatccgctg gggcgtaaca atgtgcgtac cagctccagt ctgtcgttta tcagctacga
    11701 ccaggcgctc tccagcgagc cggttgcgcc tggcgcgact aaccgcagcg agcggcgagt
    11761 aaaaatgctt gaacgtccac ggcgtgaaga ggtggatacc gcggcatgga tgccgtcact
    11821 cacaaccgat aaacaaggca aagcgtattt cacgttcctg atgcctgatt cgttaacccg
    11881 ctggcgcatc accgcgcgtg ggatgaacgg cgacgggctg gtcgggcagg ggcgtgctta
    11941 tctgcgttcg gaaaaaaatc tctacatgaa gtggagtatg ccaacggtgt atcgcatggg
    12001 cgacaaaccg gcagcaggac tgtttatctt cagtcagcag gataacgaac cggtggcgct
    12061 ggtgactaaa tttgcaggtg ctgagatgcg ccagacgctg acgctgcaca aaggggcgaa
    12121 ttatatttcg ctgacgcaga atattcagca atctggcttg ttaagtgcag aactgcaaca
    12181 aaatgggcaa gtacaggaca gcattagcac aaaactgtct tttgtggata acagctggcc
    12241 cgttgaacag cagaaaaacg tcatgctcgg cggtggcgat aacgcgctga tgttgcccga
    12301 gcaggcgagc aatatccagc tacaaagtag cgaaacgccg caggagattt tccgtaacaa
    12361 tcttgatgcg ttagtcgatg aaccgtgggg gggcgtgatc aacaccggta gccgtctgat
    12421 cccgctcagt ctcgcctggc gttcacttgc cgatcatcaa agtgccgccg ctaacgacat
    12481 tcgtcagatg attcaggata accgtctgcg gctgatgcaa ctggcggggc ccggagcgcg
    12541 ctttacctgg tggggtgaag atggcaatgg cgacgccttc ctgacggcat gggcatggta
    12601 cgccgactgg caggccagcc aggcgctcgg cgtaacgcag caaccggatt actggcaaca
    12661 tatgctcgac agttatgccg agcaggcaga taacatgccg atattgcatc gcgcgctggt
    12721 gctggcgtgg gcacaggaga tgaatctacc gtgcaaaacg ttgttgaaag ggttggatga
    12781 agctatcgcc cggcgcggaa ctaaaactga agatttctct gaggaagaca ccagcgatat
    12841 caatgacagc ctgatcctcg atacaccgga atctccactg gcagatgcgg tggcaaacgt
    12901 cttaaccatg acgttgctga aaaaagcgca gttgaagtcc acggtgatgc cacaggttca
    12961 gcaatatgcg tgggataaag cggcaaacag caatcagccg ctggcgcaca cggttgtgct
    13021 gctcaatagc ggtggtgacg ctacccaggc ggccgctatt ttaagtggtt tgaccgctga
    13081 gcaatccact attgagcgcg cgctggccat gaactggctg gcgaaatata tggcgacaat
    13141 gcctccagtt gttttgcctg cgcctgcggg cgcatgggct aaacataagt taactggagg
    13201 gggcgaagac tggcgttggg ttggtcaggg tgtgccggac attctctctt ttggtgacga
    13261 attatcgccg caaaatgtgc aggtccgctg gcgtgaaccg gcaaaaacgg ctcaacaaag
    13321 taacattccg gtgaccgttg aacgccagtt gtatcggctt atccccggtg aagaagagat
    13381 gagctttact ctgcagccgg tgaccagcaa tgagattgac agcgatgcgc tgtatctcga
    13441 tgaaattacg cttaccagcg agcagaatgc cgttctgcgc tacggtcagg tggaagtacc
    13501 gctcccgcca ggggctgacg ttgagaggac tacgtggggc atttcggtca ataaaccgaa
    13561 cgctggaaaa cagcaggggc agttgctgga aaaagcgcga aatgaaatgg gcaaactggc
    13621 ctatatggtg ccggtgaaag aactgacggg aacggtcact ttccgccatt tgctgcgctt
    13681 ctcgcaaaaa gggcaattcg ttctgtctcc tgctcgttat gtgcgttcct atgcacctgc
    13741 acagcaaagt gttgcggcag ggagcgaatg gaccgggatg caggtgaaat aagtgaactg
    13801 gcgcagaatc gtctggctat tggcgttggt tactctgcca acgcttgcgg aggagacgcc
    13861 gttacaactg gtgctgcgcg gtgcgcagca cgatcaactg tatcagctgt cgtcatcagg
    13921 cgtgaccaaa gtctcggcat tacctgacac gctcaccacg ccgttaggga gtttgtggaa
    13981 actgtatgtt tacgcctggc tggaggatac ccatcaaccg gagcagccct accagtgtcg
    14041 cggaaactcc ccggaagagg tttattgttg tcaggctggg gagagtataa cgcgcgatac
    14101 cgcgctggta cgttcctgtg ggctctattt tgcgccgcag cggttgcata ttggcgcaga
    14161 tgtgtggggg caatattggc aacagcgtca ggcacctgcg tggttagcgt ctcttactac
    14221 gctgaagcca gaaacctcgg taacggttaa ctcgctgctt gattcattag cgacgttacc
    14281 tgcgcaaaac aaagctcagg aagtcttact ggatgtggtg ctggacgagg cgaaaatcgg
    14341 tgtcgcatcc atgctcggca gtcgggtacg ggtgaaaacc tggagctggt ttgccgatga
    14401 taaacaggaa atccgtcagg gtggttttgc cggctggtta accgatggta caccgctgtg
    14461 ggtgactggc agtggcacca gcaaaacggt actgacccgt tatgcgacgg tgcttaatcg
    14521 ggttttgcca gtgccgacgc aagttgccag tgggcaatgt gtcctggtgg agctgtttgc
    14581 ccgctatccg ctgaagaaaa tcacggcgga aaagagttcg acggcagtaa aacccggcgt
    14641 acttaatggg cgctatcgtg tgacgtttgc caatggtaac cacattatgt ttgtcagcca
    14701 cggtgagacg accttactga gcgaaaaggg caaactgaaa ttgcagtcgc atctcgatcg
    14761 tgaagagtac gttgccagag ttctggatcg tgaggcgaaa agtacgccac ctgaggcagc
    14821 gaaagccatg accgtggcga tacgcacttt tttacagcaa aacgccaatc gggaaggcga
    14881 ttgcctgacc atccctgaca gcagcgccac gcagcgtgtt tctgcttcgc cagctaccac
    14941 gggggcgcga acgatgaccg cctggacgca ggatcttatc tatgcgggcg atccggttca
    15001 ttatcacggc agtcgcgcta ccgaagggac gctttcctgg cggcaagcca cggcccaggc
    15061 ggggcaggga gagcgttacg atcagatact cgcgtttgct tatcccgaca atagccttag
    15121 ccgctgggga gcgccacgct caacctgcca gttattaccc aaagcgaaag cctggctggc
    15181 gaaaaaaatg ccgcagtggc ggcgtatatt acaaggtgag acggggtaca acgaaccaga
    15241 cgtgtttgcg gtttgccgtt tagtctcagg tttcccctat accgatcggc agcagaaacg
    15301 gctgtttatt cgtaacttct ttacgcttca ggatcggctg gatttaaccc atgagtatct
    15361 gcacctggcc tttgatggtt atcccaccgg gcttgatgag aactatatcg aaacgcttac
    15421 ccgacaatta ttaatggact aatgctatgc gaaagatttt tcttccgttg ttactggtgg
    15481 cgctttcgcc tgtcgctcac agtgaaggtg tgcaggaagt cgagattgat gcaccgctct
    15541 ctggctggca tccggcagag ggtgaagatg ccagtttttc acaatcgatc aattacccgg
    15601 cgtcgtccgt caacatggca gatgatcaaa atatctcagc gcagatccgc ggcaaaataa
    15661 agaattatgc tgcggcgggt aaagttcagc agggccggct ggtggtcaac ggtgccagta
    15721 tgccgcagag aattgaatcc gatggttcat ttgcacgccc ttatattttc acggaaggca
    15781 gcaacagcgt acaggtcatc agcccggatg ggcaaagccg gcaaaaaatg cagttttact
    15841 caacgccggg tacaggaacg attcgtgcac gtttacggct ggttctctcg tgggatacgg
    15901 acaataccga tctcgatctc catgttgtca cgcccgatgg cgaacacgcc tggtacggta
    15961 acacggtgct gaaaaacagt ggtgcactgg atatggatgt cacgacgggg tacggacccg
    16021 agattttcgc catgccagcg ccgattcacg gacgttatca ggtgtatatc aactactatg
    16081 gcggacgcag cgaaacggaa ttgacgaccg cccaactgac gctgatcacc gatgaaggat
    16141 cggtcaatga gaaacaggaa acatttattg taccgatgcg taatgctggc gagctgacgc
    16201 tggtgaaaag ttttgactgg tgattaatgg ggttatcttt actgtcaatg ttcaagaaaa
    16261 cgcccgatga ctttcgctat cgggcgtttt tacatgttaa ttcaatcgtt caatcaccat
    16321 cgcaattccc tggccgccgc caatgcacaa cgttgccaga cccagcgttt tatcgcgtgc
    16381 ctgcatggca tgtaatagtg tgaccagaat acgcgcaccg ctggcgccga taggatgccc
    16441 cagcgcgatg gccccaccgt tgacattcac tttctcagga tcaaagccca gggttttccc
    16501 aacggcaagg aactgtgcag caaatgcttc attggcttca ataagatcaa tgtctgccag
    16561 ctgcagcccc gccagttgta acgctttttg cgtggcaggc actggcccca tacccatcaa
    16621 tgcggggggc acgccaccgc tggcataact tttaatgcgt gccaggggat tcaggcctgc
    16681 tgccagcgcc gcagattctt ccataatcac cagagcggca gcaccgtcgt taatacctga
    16741 cgcgttccca gcggtgactg ttcctgcttt atcgaaggcc gggcgcaatg cgcctaacgc
    16801 ttcagccgta gaatccgctt tcgggaattc gtcttgactg aagacgaggg ttttcttccg
    16861 agtgacaaca tttaccggga cgatttcggc tgtaaaagca ccggactcaa ttgcggctgc
    16921 cgctttacgc tgtgaatgta gcgccagttc atcctgcatt tcacgggtaa ttccgtactc
    16981 tttagccacg ttttcggcgg taatccccat atgataacca tgggtggcgc acatcagacc
    17041 atcgcgcagg attacgtcat aaacctgtcc gtctccaaga cgataaccag agcgtgcttt
    17101 tgcatcgagt aagtagggcg ctaaactcat attttccata ccccccgcca caatgctctg
    17161 tgcctgacct gcctgaatcg cctgtgcagc aagcgccacg cttttcagac ctgaaccgca
    17221 cactttgttg accgtgaatc cgcacaccgt ttcagccagt ccgcttttta gcagcgcctg
    17281 acgtgccgga ttttgcccca gcccggcttg tagcacgtta cccataatca cttcatcaac
    17341 gtgtagtgaa tcgatttttg cacgttcaat ggcggcttta attactgtcg cccccaggtc
    17401 gatggcgctg gtggaagcga gtgaaccgtt aaaactaccg atagcagtac gtaccgcact
    17461 gacgatgaca caatttttca ttttatattc cttctgtctg tagggttgcc gtcagaacag
    17521 cgttaaacca atgacgaaaa tgacgccgga gaagagcagg gcagtgatgc aatagcccat
    17581 gatgtcgcgg acaccgagtc cggcgattgc cagtgctggc agcgcccaga acggttgtgc
    17641 catgttcatc cattgctcgc cgtaggcgat cgccattacc gatttaccga gatcagcgcc
    17701 cagcgcctgg gctgcgggca tcacgaacgg cccctgaata acccagtgcc cgccgccaga
    17761 cggaacggcg aagttaatca gtgcagaact aaaaaaggtc atgaccggga aggtgtcttt
    17821 gttcgcaaca ttgatgaaga attcggtaat gagtccgccc agaccggagt gctccatcat
    17881 cagttggatc cccgcgtaga aggggaattg caccagaata ccggcggtac tgcgtgctgc
    17941 cgcgctgata gcacgcatat aagccattgg cgttttatgt agcagcagac ccgcaatcat
    18001 aaacatcagg ttgacggtat tgatggtgat gttgaagcca tgttcgctga agtacatcgc
    18061 caggtaagcg ataccgagtg cgccgatgat caacgtcaga atgcggcttt cttccagtcg
    18121 ctcggacggt ggagcatctt tcggtagttg cttttgaaaa tcagcctctt ccatgagtag
    18181 tttcggatcg atactcacca cgtcagacgg ttttggcatc atcatgcggg tgataaatgg
    18241 catcaccaca atcaacgcca cagtgatgaa aatattaaaa ccactgaaca gagtatcgcc
    18301 caccgggatc agcccggcga tatgctcaac cgggttgccc ggtgttgcag ccaacagagg
    18361 cattgagcca gagaaaccgc caccccaggt gagaaaacca atgtaggcgc aggcaatgag
    18421 caacggataa tcagaaccgg gtactcgccg ggcgacttca cgggcgaaca ttgcaccgac
    18481 aaccaaacca aatccccagt tgatgacaca agcgactgaa ccgaagaaag taaccagcat
    18541 gacgccctgt acgggcgtct ttgcggcgga ggcggcagta cgcagcaaac ttttcaccgg
    18601 agcggagctg gcaagggcat gaccggtaac gatgataagc gccatctgca taccaaacgc
    18661 cagcaagttc cagaaaccat caccccacat tttcaccatg ctgatcggcg tttgtggtgt
    18721 taaccaaagc gcgatcacga atgttagcaa tgtcagcaac atggcaaaga tcagtggatc
    18781 gggaagccac cggctgacaa aacgcgtcat aaaacgcgat atgcgaccaa tcataaatca
    18841 ccccgttgcg tattcagatc ggcggcaact tcaaaccgag cttctgtttt ggcacgcacg
    18901 gtggctaaat cacacccgtc ggcaatttcg gtgagccaca ttttgccgtc aataaaacga
    18961 aagacagcca gttcagtaac cagcatatgc accgcatgtt gcgcagtgag tggcatggtg
    19021 cagcggcgca aaatttttgc tgaaccatct ttggcgcaat gttccatggc gatgatcact
    19081 ttgcgtgacc cggtcaccag atccatcgcg ccacccatac cgggcaccat tttcccaggc
    19141 actacccagt tcgcgaggtt tgcttcttcg tctacttgca aaccgccgag cacgcaggca
    19201 tcaatatgac cgccgcggat tagcgcaaat gacatggcgc tatcaaacat ggctgcgccg
    19261 ggtaaaatgc cgcacggttg tccgccagcg ttcaccagat ctggatgcgc tgtcgtgacc
    19321 gggcctaaac cgaggaagcc gttttccgat tgcagagtga tatgaatacc ctccggtaaa
    19381 taattggcga ccattgtggg taaaccgatc cctaagttaa cgatgtcacc atcacgaagc
    19441 tcttgcgcca cacggcgcgc aatacgttgt ttcgcatcca ttatttgctc tcctgtgaaa
    19501 cgatgatgtg gtcgataacg gcaccagggg tgacaatatg gtcaggttgc agctcgccgg
    19561 tttcgaccag ttcatctggc tctaccagcg tgatatcagc cgcaagggct atcagggggt
    19621 taaagttgcg ggcgctaagg tgataggtca ggttgccaag tgtgtcgcaa cgatgagcgc
    19681 gaattagcgc caggtcggcg cgcagcgggc gttcgagcag ccaggtttta ccgtcgagtg
    19741 tcagtgtctg tttgccttcc tctacgacgg tgccgacacc cgttggggtg agaaaaccac
    19801 caagtccagc tccgccacag cgaatttgct cgattagcgt accttgcggc accagaacga
    19861 cgtccatctc accagatatc atgcgccgac ctgtttccgg gttggtgccg atatgtgaag
    19921 caatcacttt gcggactcga ccattgacga tgagcggacc gatgccggta tcaacaaacg
    19981 cggtatcatt ggctatcaat gtcaggtcgc gaacaccaga ttccagtaat gcttcaacca
    20041 ggcgggatgg agtgccaatc cccataaatc cgcccaccat gatggtcatg ccgtcacgaa
    20101 agaagccggt ggcgtcttgt aatgtcatca attttgtttt cattttttat ccctcttgca
    20161 tacaccgttg tgggtacagt cagggaatag caaggggagt gccagaatga gattattgga
    20221 ttttgttatt taactaattg aatatgaagg gataaattgg atggagattt ataaggagaa
    20281 tgagcggcta tgcagaaaat tgcgcactgt gcaaatttct gcatagcaag ttttggcgtt
    20341 atacatccgc cggatcgata ccgtattcct ggagtttata catcaatgca cggcgactga
    20401 tgcccagcat taaagcagtg cgggttcggt ttccttcttg ttgttccagc acttccataa
    20461 tgatgcgttt ttcgacgcgt ttaatttcct cttttaaatt acgctcaccg acaggggctg
    20521 tttttgcttc accagcatta cagactggct gacgaatctg cggcggaaga tcctcagaaa
    20581 aaatgatcgg gcctgaattc atcacgacgg cgcgttcaat aacgttggaa agctctcgaa
    20641 tatttcccgg ccatgaccag gcggtaagca gtgacattgc catcggatcg atgtcgataa
    20701 tatcgcgctg attctcacta ctgaattttt gcaaaaagtg attagctaac agggaaatat
    20761 cttcccggcg atcgcgcaga ggcggcagta ttaaatgaat aacgttaagg cgataaaaga
    20821 gatcttcacg gaaggtgcct tcttttacca ttgcctgcaa gtcacggttg gtggcagcaa
    20881 tgatgcggat atcaactttt atggtctgat ggccgccaat ccgttcaaat tcccgttcct
    20941 gcagaatgcg tagtaatttg gcttgcagta cgagcggcat ttcgccaatt tcgtcgagga
    21001 gcagagtacc ttcgttggca cgttcaaata atccctgacg caaggtttgt gcaccagtaa
    21061 atgcaccttt ttcatgacca aacagttcac tttcgagcaa cgattccggc agcgccgcgc
    21121 agttgacttt aatgaacggc ccctttgccc gccgcgaatt gtagtgaatc gctctggcaa
    21181 tcaactcttt cccggtgccg ctttcaccgc taatcaagac gctggcctga gaaagggcaa
    21241 ttttggcggt gtctttgcag atatccatca tcgccgggct gttggtgaga atgtgccccc
    21301 attgccagct ggtgctcagt gcctggtgca gatgacggat ctcttttttc attgactgga
    21361 gttgtaaagc gcgctgaacg attaaattca actcatcgag atcaaacggt ttaataacat
    21421 agtcgaaggc cccgcagcgt agcgcttcga cggcggtttc cacttcagca taggccgtca
    21481 tcagaataac gggtgtccgg gtctcattac tgcgcatctc ctttagtgcc ttgatgccgt
    21541 ccatctctgg catgcggata tccatcaaca ccacgtcagg gtgaatatca gcaaacaggt
    21601 gtaatgctgt gcgtccgttg ttcgcacaat gtgtttcgaa tccttgtagt gcaaaagcgg
    21661 tgctcagcat acggcgaaca ttatcttcat catccacaat aaggatgcga ttaatagcag
    21721 tcatacagtc tgatttccct gcgggttgat cggtaaaata agcgtgaagg ttgcgccgta
    21781 gcccggcaaa ctggcgacgc gaatatcacc ctgatgggca ttaatgatgc gttgacttaa
    21841 cgccagacca agcccggttc ctgaggcttt ggtagtgaaa aagggatcga agatcttttt
    21901 ttgcagcgag agatcaatgc cacagccgtt gtcctctatc gaaatggcct gttgtgagtc
    21961 gctgtactgc caggttcgaa tgcgaatttt ccctcgtgca ctgatagcct ggacggcatt
    22021 gatcaggata ttcagtagta cctgtttgag cagttcacga tcggcgttaa tcgggcttaa
    22081 ttcattatcc agttcgctta tgaagtcgat ccgcgcttgt acgccagcgg tttgtaccag
    22141 taccagagtt tcttcaacca atgcattgag gctgacttgt tgccattgac tgtggcgtgg
    22201 acgtgaaaat tcgagcaatt gctgaataac tttgttaatt gaatcgattt ctttgagtac
    22261 tacggacaga tattcctgat gtattgggtc accggtttgt tggcgcaaga tctgtacata
    22321 accacgaata gccgttagcg gattacgtac ttcatgcgcc acgccagcca tcagctcacc
    22381 cagtgtggcg aggcgttctg cttgcgccat gcggcgctgg gtttctttgc gggcagttaa
    22441 atcagagaaa atcaccaaag cacctatcat atcaccatgc gtgttatgaa tacgactggt
    22501 agtgacactg agttcaatgg tgcggtcacg gcctggaaaa ctgatctcca gcgccacatg
    22561 ttcggtgcca tgttccagcg tatccagtac tggactgtag aactgagtat ggtcgaacaa
    22621 catggagtaa ggctgcccta ccagttcatg acgttgatag ccagtgatga cttctgctgc
    22681 cgggttcatg gtggttacat caccctggcg gtcaatggca atgacgccat cggcagcgtt
    22741 ttcaataatc agatcgttaa gtgtccgcgt ttcacgcagt gcctgggcga ggttgttaac
    22801 actctgactg atttgcccca tttcaccggg caattgtggt aatcgagtgg gaatattttg
    22861 cgccagagtc gagaggccat cggtgatgat atcaatattg gcgctcaggc gacgggagaa
    22921 aaggacaatc aacagcaggc ttatcagcaa accggcggtg agcacaatga taatcctcac
    22981 atccattttc caggcctggc ggcgaatatc ttcggttaat tcattggccc agatatagcc
    23041 gaggatttca ccattacgct caatgggaat cattgaattc aaaatatcgc cgcgtacctg
    23101 cctgcctgaa taaaccaaag gggtatttgt acgcatgact tcgcgaccag ggtgatctgc
    23161 ggcaatggta acgcccacat tattctgata tagcgctgaa ggcgcgtagg tgattatcgc
    23221 atccagcgtt ttgttgtaat aaccagcgcc gatgccaggg aaggcgtgag tgatattttc
    23281 ggtaatgggg gcaagttctg catttaatgc gcggatacgc tcctcacgcg gtaagtcgat
    23341 gtagagatcg tagcgattgc ctagcgcctg attaagcagg ttgaccacgg cagaaagttt
    23401 tttctctttt tcagataaga ctgctgaacg tccttctgtt tctacgatat aaccaatagt
    23461 aagcgttggg acaatgacca tcaggattgc catcagaatc atttgattgc gtaagcggcg
    23521 tggataaatc cacttcatat aatgcatgct gaaatccact agtcttgtcc ggtatatgac
    23581 gattatcaga ggttaaggtg atgatttctc ggcggtgtat catattccag agaagagaga
    23641 acattgcggt aacacgcttt taccgctacc ttaaccacac tccatcggtc acctgaggcg
    23701 gagcttcgcc cctttgaaat accttgcttc ttttcgtaca accctgaaag cctcgcgcta
    23761 catgttcaga gcattggcgt tagtgctctg gctgttgatt gctttttcat ccgtttttta
    23821 catcgtgaat gcgttacatc agcgagaatc ggaaattcgt caggaattta atctgagttc
    23881 cgatcaggct cagcgcttta ttcaacgcac ctctgatgtg atgaaagagc tgaagtacat
    23941 tgccgaaaat cgcttatcgg cagaaaacgg tgtgctttcc ccgcgtggac gagaaacgca
    24001 gacggatgtg cctgcgtttg aaccgctgtt tgctgactct gattgttccg caatgagtaa
    24061 cacctggcga ggctctctgg agtcattggc gtggtttatg cgctactggc gcgataattt
    24121 ttctgcggct tacgatctca accgggtatt tttaatcggc agcgataacc tctgcatggc
    24181 caatttcggt ctgcgtgata tgccagtgga acgcgatacc gcgttgaaag cgttgcatga
    24241 acgcatcaat aaatatcgaa atgcaccaca agatgatagc ggcagtaacc tctactggat
    24301 cagcgaaggt ccgcgccctg gcgtcgggta tttttacgcc ttgacgccag tttatctggc
    24361 gaaccggttg caggcgcttt tgggtgtcga gcagactatc cggatggaga attttttctt
    24421 acctggtacg ttgccgatgg gggttaccat tcttgatgaa aatggtcata ccctgatttc
    24481 gcttaccgga ccagaaagta aaattaaggg cgatcctcgc tggatgcagg aacgctcctg
    24541 gtttggctat acggaagggt tccgggagct ggtgctgaag aaaaatctgc caccctcatc
    24601 gctaagcatc gtgtattcgg tgccagttga taaggtgctg gaacgcattc gcatgttgat
    24661 ccttaacgca attttgctga atgtgcttgc cggagctgca ttgtttactc tcgcgcggat
    24721 gtacgagcgg cgtattttca ttccggcgga aagcgacgcc ctgcgactgg aagaacatga
    24781 gcagtttaat cgcaagattg tcgcctccgc gccagtgggt atctgcattt tgcgtaccgc
    24841 tgatggcgtc aatattttaa gtaacgaact ggcgcatacc tatctcaata tgcttacgca
    24901 tgaggaccgc caacggctga cacaaattat ctgtgggcag caggtcaatt ttgttgatgt
    24961 cctgaccagc aacaatacca acctgcaaat cagcttcgtc cattcgcgct atcgtaatga
    25021 aaacgtggcc atttgtgtgc tggtggatgt ttcttcgcgc gtgaagatgg aagagtcgtt
    25081 gcaggagatg gcacaagcag cggaacaggc gagccagtca aaatcgatgt tccttgccac
    25141 cgtcagtcat gagctgcgaa cgccgctgta tggcattatc ggtaacctgg atctgttgca
    25201 aaccaaagag ttaccgaaag gcgtcgatcg tctggtgacg gcaatgaaca actcttccag
    25261 cctgttgttg aaaattatca gcgatattct cgacttctcg aagattgaat cggaacagtt
    25321 gaagatcgaa ccgcgtgagt tttcaccgcg tgaagtgatg aaccacatca ccgccaacta
    25381 tttaccgctg gtggtacgca agcagctagg cttgtactgc tttattgaac cggatgtgcc
    25441 agtggcctta aatggcgacc cgatgcgttt acagcaggtc atctccaacc tgttgagtaa
    25501 cgccataaaa ttcaccgata ccggctgtat agttctgcat gttcgcgctg atggcgatta
    25561 tctctctatc cgtgttcgcg ataccggcgt agggattccg gcgaaagaag tggtgcgctt
    25621 gtttgatccc ttcttccagg tcggaacggg cgtacagcgt aacttccagg ggaccggtct
    25681 gggtctggcg atttgtgaaa aactgatcag catgatggac ggcgacatct cggtagattc
    25741 agaaccggga atgggcagcc agtttaccgt gcgtattccg ttgtacggcg ctcagtaccc
    25801 gcagaaaaaa ggcgtggaag ggttgagtgg taaacgttgc tggctggcgg tccgcaatgc
    25861 gtcgctctgt cagttcctgg aaaccagttt gcagcgcagc ggcatcgtcg ttacaacata
    25921 cgaagggcag gaaccgactc ccgaagatgt gttaatcact gacgaggtag tgagcaaaaa
    25981 atggcagggt agagcggttg tgaccttctg tcgtcgccat attggtattc cgctggagaa
    26041 agcgccagga gagtgggtac acagtgtggc tgccccgcat gagctaccgg cattgttggc
    26101 gcgtatttat ttgatcgaga tggagagcga cgatcctgct aacgctctgc cgttaacgga
    26161 caaagcggtc agcgataatg acgatatgat gattctggtc gtggatgatc atccgattaa
    26221 tcggcgtctg ctggcagatc agttgggatc gttgggctat caatgtaaaa ccgcgaatga
    26281 tggcgtcgat gcgcttaatg tacttagcaa gaatcatatt gatatcgtgc ttagcgacgt
    26341 caacatgcca aatatggatg gttaccgctt gacgcaacgc attcgtcagt tgggactgac
    26401 gttgccggtt atcggagtaa ctgctaatgc gttggctgaa gagaagcagc ggtgtctgga
    26461 gtccggtatg gacagctgcc tgtcgaagcc ggtaacgctg gatgtgataa aacagacgct
    26521 aacggtatat gccgagaggg tcaggaaatc gcgggattcg taggacggat aaggcgttta
    26581 cgccgcatct ggcattcagt gcaaatgcca gatgcgacgc tgacgtgtct tatctggcct
    26641 actttaatgt aatctgttga aataatggga atcgtaggac ggataaggcg tttacgccgc
    26701 atccggcaat cggtgcaaat gccagataag acactaacgc gtcttatctg gcctacaggt
    26761 gattagtctt tatctgccgg acttaacgtc actgaagaga gataattcag cagagcaata
    26821 tcgttctcga cacccagctt catcatcgca gatttcttct ggctactgat ggttttgata
    26881 ctgcggttca gctttttagc gatctcggtc accaggaaac cttccgcaaa caggcgtaga
    26941 acttcactct cttttggcga gagacgcttg tcaccgtaac caccagcact gattttttcc
    27001 aacaggcgag aaacgctttc cggggtaaat ttcttccctt tctgcagcgc ggcgagagct
    27061 ttcggcagat cggtcggtgc accttgtttc agcacgatcc cttcgatatc cagatccaat
    27121 accgcactaa gaatcgccgg gttgttgttc atagtcagaa caatgatcga caggcttggg
    27181 aaatggcgct tgatgtactt gattaaggta atgccatcgc cgtacttatc gccaggcatg
    27241 gagagatcgg taatcaacac atgcgcatcc agtttcggca ggttgttgat cagtgctgta
    27301 gagtcttcaa attcgccgac aacattcacc cactcaattt gctcaagtga tttgcgaata
    27361 ccgaacaaga ctatcggatg gtcatcggca ataattacgt tcatattgtt catgtaatag
    27421 gctaccttgc tacagcaagc tcttgacata actgtcaatg tcgctgatgt atttttctat
    27481 tcctggaaca tccttctcac gaatcagatg ttccagcgtt tcacataact gcttgccggg
    27541 taccagattt agcatggcaa atacgccttt aagacgatga gccgtttggg ctaacgcagc
    27601 aaagtcactg gttgctgctt cagtatacag cctcttaaca tcatccggta ctgtgtctac
    27661 aaagagcgca taatagccgc tggcatggag ttgcgcattt tcatctccgc ccagaggcga
    27721 ttctgtcacc tcttcctgcg ccagttgcac ttcaattaat tgtaagaccg cttcctgcat
    27781 agcgttgctc atattgaagt tgacgcacaa ttgaccaggc ccaatttccc gtacgccaga
    27841 ctcatcatcg cttaaaagca agccagaggc agtaagatta gacggattat ccgttaaaaa
    27901 gatatcataa tcttgactaa ttaatctttc atcgggtgtg atacaggttg caccccaatt
    27961 ttctaactgg cgagtgacaa tattccgaat ttctgccgaa gtaacatcca ccattacgca
    28021 gacatcatcc agtaaacgct cttcttcctc ttcaacttcc gggtcagctg cgagcatttt
    28081 gatatgcaca gagtagcgag taccaagccc atcccgcgtt ttgatgttta aatgaccgcc
    28141 cagtttacgc gccagttgat cgctcagcca gaatgccagc gggtccgcct tgccatagcg
    28201 atcgttttgg gtctggttga taaacgggaa gtgcaaatta tccatttcat gaatacttac
    28261 gccttctccc gtgtccagaa tgcggaacgt caggcggtct tcggaggact catcctgatc
    28321 aacctcaagg gtgatttttc ccaattgcgt tgaggtcacg gcatattgca tcagtagcag
    28381 caaaatacgt cgtaaggcat cgcgatcgcc gcggcgcata tcgtgtgctt tcagatgatt
    28441 gttaatcagc agttgcagac ctttacgctt gatggcaggc aacactgaag gcacaacttc
    28501 atcaattaaa tcctgcacgg agaacagcac cgtctcactt ttccagctat cgtccgcaag
    28561 catgttcgct aactgtattt catcgaccag ccgcaccagc acatccgcct gattcgtcag
    28621 ttgtttgcct tccggggcgt tgagtttagc cgcgctctcc gccagggact gtgcgggttc
    28681 tttcagcgca tcgccaatgt ttttcataaa ggtcatccgc ccctgctggt ttttctcata
    28741 cagacgctgc gcctgcttga gtttcttgtt taccagcact tcgcgatcct gatcgcgaat
    28801 aatgaaaatt tgtgtgcgcg gcgcaacctg gctgcggaac atgcggatct catacagctc
    28861 gttattgatc gtcgcctgaa taatcccctg atgctgttcc gccatggtgg tgatgttttg
    28921 cagattcaaa tgcggcagca aatgatcggc aattttgtta cttatgacag tgcggttcga
    28981 ttcctgatcg tgaaccagca ggccgagcgg cagtagtgag actatctctt cattgattgc
    29041 ccgtaaaatg cgcaattcgt tattgaccgc cgtgttgggt agactttctg tactgcgact
    29101 ggagaaatgg cgaaatgtgg tatagccaaa taacgccagc gccagcaaac cgatgttcag
    29161 cagcagtggc agcagaatgt tttgcaacgt atccagcaat aaggtgccat aaggaacctg
    29221 ccagaccagg cgcatatcgg tagagttgag tgccgaggag atttcaatct tcgtactgtt
    29281 aaagtggata ctgacgctat ccgtcccttc tttctcatta tcattgtctc ccgttgccgt
    29341 cgcgtctggc tcaaggcgga aactgtccag cggcataccc ggtgggatca aatcattaat
    29401 cggcagatca aaagccacaa ccgttgccag atgtcccggc tggttgaacg tggtacgcaa
    29461 ggtaaagtaa tgaccgttct gccaggccag gcggcgcatg ttagaaaagc tttcgcgttc
    29521 atcgagggcg ttggcctgtt gcaacatctc tgcacgacgt gaatcaacaa tgtcgctgac
    29581 ggtcgattct ttaaatccgg aggtgagatc tttgaggggc agggttgaga tcagcaccag
    29641 actgttatcc tgaccattca ggtaatacat cgaccacggt acattttctg cgccccacaa
    29701 tgtatccaga taagtggaca tccgttgagt catctcaagc gttgagttgt cgtgagagcc
    29761 aaagattaac gcttccgttt tgcggcgtgg tttctccaga tagtagacat cctgtttcag
    29821 gcgcgtctct tgtaaacctt cgccggagga gggggtggtc gtcgcggcaa tgttgtcgta
    29881 gatctgccag gtcacgtaac gccaggtatc gacgcgcttt tggatagcgt gggtaatgtc
    29941 gacaatctgg taacttttat ctttcagcca ggcgttaacg gcgctttgta ccattacacc
    30001 catcgtcacc agtaacacaa tgatcaacag taaaaagaag cgggtaatgc tccccggtag
    30061 gagtgaaaag cgggtcgtgg ccgttgtctc tttctgacgc attcgtgttt atgacctgtt
    30121 aaaacttcgc gaagtgatag ggcagtataa agggtacagt gtgaaattcc agactcttac
    30181 cggattgatg attctctatt ctcccgtgat atttatcatc ctgcattggg ggaaatagat
    30241 gtacaagcgc cattttgctg tacaaataac ctacaaaaag cctaacgaaa ttaattgcaa
    30301 ataaacgaag attgctggaa attatgctga tgttaattat ttgtgaaata gttaacaagc
    30361 gttatagttt ttctgtggta gcacagaata atgaaaagtg tgtaaagaag ggtaaaaaaa
    30421 aaccggatgc gaggcatccg gttgaaatag gggtaaacag acattcagaa atgaatgacg
    30481 gtaataaata aagttaatga tgatagcgga agatattcta gttgcgagtg aaggttttgt
    30541 tttgacattc agtgctgtca aataattaag aataagttat tgattttaac cttgaattaa
    30601 tattgcttga tgttaggtgc ttatttggtc attccgcaat aatcttaaaa agttcccttt
    30661 catttacatt ctgaaacatc tatagcgata aatgaaacat cttaaaagtt ttagtatcat
    30721 attcgtgttg gattattctg catttttggg aagaatggac ttgccgactg attaatgagg
    30781 gttaatcagt atgcagtggc ataaaaaagc aaataaaggc atataacaga gggttaataa
    30841 catgaaagtt aaagtactgt ccctcctggt cccagctctg ctggtagcag gcgcagcaaa
    30901 cgctgctgaa gtttacaaca aagacggcaa caaattagat ctgtacggta aagtagacgg
    30961 cctgcactat ttctctgacg acaagtctgt agatggcgac cagacctaca tgcgtcttgg
    31021 cttcaaaggt gaaactcagg ttactgacca actgaccggt tacggccagt gggaatatca
    31081 gatccagggc aacgctccgg aaagcgaaaa caactcctgg acccgtgtgg catttgctgg
    31141 tctgaaattc caggacatcg gttctatcga ctatggtcgt aactacggcg tagtttacga
    31201 cgtaacttcc tggaccgacg tactgccaga attcggtggc gacacttacg gttctgacaa
    31261 ctttatgcag cagcgtggta acggcttcgc gacctaccgt aacactgact tcttcggtct
    31321 ggttgatggc ctgaactttg ctgttcagta ccagggccag aatggtagcg taagcggtga
    31381 aagtgatccg gatttcactg gccatggcat caccaacaat ggtcgtaaag ctctgcgtca
    31441 gaacggcgac ggcgttggcg gatctatcac ttatgattac gaaggctttg gtatcggtgg
    31501 tgcgatctcc agctccaaac gtacctggga tcagaacaac actggtctga tcggtactgg
    31561 cgaccgtgct gaaacctaca ctggtggtct gaaatacgac gctaacaaca tctacctggc
    31621 tgctcagtac acccagacct acaacgcaac tcgcgtaggt tccctgggtt gggcgaacaa
    31681 agcacagaac ttcgaagctg ttgctcagta ccagttcgac ttcggtctgc gcccgtctgt
    31741 agcatacctg cagtctaaag gtaaaaacct gggcaccatc ggtactcgta actacgacga
    31801 cgaagatatc ctgaaatatg ttgatgttgg tgctacttac tacttcaaca aaaacatgtc
    31861 cacctatgtt gactacaaaa tcaacctgct ggacgacaac cagttcactc gtgacgctgg
    31921 catcaacact gataacatcg tagctctggg tctggtttac cagttctaat ctcgattgat
    31981 atcgaacaaa gggcctgcgg gccctttttt cattgttttc agcgtacaaa cacagttttt
    32041 tggtgtactc ttgcgaccgt tcgcatgagg ataatcacgt atggaaataa gctttacccg
    32101 cgtggcactg ctggctgccg cgctcttctt tgttggttgc gatcaaaaac cacaacccgc
    32161 caaaacccac gctactgaag ttaccgttct tgaaggcaaa accatgggta ccttctggcg
    32221 tgccagcatt ccgggcattg acgccaaacg cagtgccgaa cttaaagaaa agattcagac
    32281 ccagctggac gccgacgatc agctgctttc gacctataaa aaagattccg cgctgatgcg
    32341 ctttaacgac tcgcaaagtt tgtcgccgtg gccagtaagt gaagcgatgg ccgatatcgt
    32401 caccacctcg ctacgcattg gcgcgaagac cgatggcgcg atggatatta ccgtcgggcc
    32461 gctggtgaat ctgtggggct ttggcccgga acaacagccg gttcaaattc cgagccagga
    32521 acagatcgat gcgatgaaag ccaaaaccgg cttacagcac ctgacggtga ttaatcagtc
    32581 ccaccagcaa tatctgcaaa aagacctgcc ggatttatat gtcgatctct ctaccgtcgg
    32641 tgaaggttat gcggcggatc acctggcacg cttgatggag caggaaggga tttcccgcta
    32701 tctggtgtcg gtgggcggcg cgctgaacag ccgtggtatg aacggtgcag gccagccgtg
    32761 gcgggtagcg attcaaaaac caaccgataa agaaaacgcg gttcaggcgg tggtggatat
    32821 caacggtcat ggcattagca cctctggcag ctaccgtaac tattacgaac tggacggcaa
    32881 acgtctttcc catgttatcg atccgcaaac cgggcgtcct atcgaacaca atctggtatc
    32941 cgtgacggtg attgccccga cggcgctgga ggccgatgcc tgggatactg gcttaatggt
    33001 gctcgggccg gagaaagcca aagaggttgt tcgccgggaa ggactggcgg tctacatgat
    33061 caccaaagag ggtgatagtt ttaaaacctg gatgtcgccg cagtttaaaa gcttccttat
    33121 cagcgaaaaa aattaaagcg caagattgtt ggtttttgtg caatggtgac cgggcagact
    33181 aaaggctatc cttaaccagg gagctgatta tgaaaaacgc cacatgctta actgacgatc
    33241 aacgctggca atctgtctta gcccgcgacc cgaatgccga cggcgaattc gttttcgccg
    33301 tgcgcaacac aggcatcttt tgccgtccgt cttgccgcgc cagacatgct ttgcgggaaa
    33361 acgtttcttt ctatgccgat gccagtgagg cgcttgccgc tggctttcgc ccctgcaaac
    33421 gttgtcagcc agacaaagcc aatccccaac aacatcgcct tgataaaatc acccacgcct
    33481 gccgactgct ggaacaggaa acgcctgtaa cgctggaatc cttagccgaa cagctggcga
    33541 tgagtccgtt ccatttgcat agattgttta aagcgactac cggaatgacg ccgaaagcct
    33601 ggcagcaggc ctggcgtgct cgccgattgc gggagtctct ggcaaaaggg gagagcgtga
    33661 ctcaggcgat tctcagtgcc ggattccccg acggcagcag ttactaccgt aaagccgacg
    33721 aaacgctggg catgacggct aaacaattcc gtcacggtgg cgaaaatctg gcggtgcgtt
    33781 acgcgctggc tgattgtgag ctgggccgtt gcctggtggc agaaagtgag cgggggattt
    33841 gcgcgatatt actgggcgat gatgacgcga cactaatcag cgagttacag cagatgtttc
    33901 ccgctgccga cagcgcgcct gccgatctta cgtttcagca acatgtgcgt gaagtgatcg
    33961 ccagcctcaa tcaacgcgat acgccgctga cgttaccact ggacattcgc ggcactgctt
    34021 ttcagcaaca agtctggcag gcactgcgca cgataccttg cggtgaaacc gtcagttatc
    34081 agcaactggc taacgccatt ggcaaaccga aagcggtacg ggccgttgcc agcgcctgtg
    34141 ccgccaacaa gctagctatc gttatacctt gtcatcgggt ggtccgtggt gatggcacac
    34201 tttccggtta ccgctggggc gtgtcgcgca aagcgcaact gctgcgccgc gaagctgaaa
    34261 atgaggaaag gtaatgttgg atctgtttgc cgatgctgaa ccgtggcaag aaccactggc
    34321 ggctggagcg gtgattttgc accgttttgc ttttaacgct gcggagcaac tgatccgcga
    34381 tattaatgac gttgccagcc agtcgccgtt tcgccagatg gtcactccag gcggatatac
    34441 catgtcggtg gcgatgacca actgtgggca tctgggctgg acgacccatc ggcaaggtta
    34501 tctctattcg cccattgatc cgcaaacaaa taaaccgtgg cccgccatgc cgcagagttt
    34561 tcatcattta tgtcaacgtg cggctacggc agcgggctat ccagatttcc agccagatgc
    34621 ctgtttgatc aaccgctacg ttcctggcgc gaaactgtcg ctgcatcagg ataaagacga
    34681 accggatctg cgcgcgccaa ttgtttctgt ttctctgggc ttacccgcga tttttcaatt
    34741 tggcggcctg aaacgaaatg atccgctcaa acgtttgttg ttggaacatg gcgatgtggt
    34801 ggtatggggt ggtgagtcgc ggctgtttta tcatggcatt caaccgttga aagtgggggt
    34861 tcatccactc accaccgact gccgctacaa cctgacattc cgtcaggcag gtaaaaaaga
    34921 ataaaaataa gaattattat tgctgtgcgc acgaagatgt ttaaactgcg ggctgtaatt
    34981 cattgtccgg gttttctgca tggaacttct tgtacttgtc tggcggcagt atcgctggcc
    35041 atttatcagt gtgatggcgc taagcctcgc cagtgcggca ttaggcattg gcttaattgc
    35101 ttttatcaat cagcgcctta tcgaaacggc ggataccagt ctgctggtat tgccggagtt
    35161 tctggggtta ttgctgctgt tgatggccgt cactctcgga tcgcaactgg cgctcaccac
    35221 tttggggcat cacttcgttt accgactgcg tagtgaattt atcaagcggg ttctggatac
    35281 ccacgtcgag cgcattgaac aacttggtag cgcttcgttg ctggcggggt taaccagcga
    35341 tgtgcgcaat atcaccattg cttttgtgcg tctgccggaa ctggtgcagg ggatcattct
    35401 cactatcggt tccgcggcgt atttgtggat gctgtcgggc aaaatgttgc tggtaacggc
    35461 tatctggatg gcagtcacca tctggggcgg ttttgtgctg gtggcgcggg tgtacaaaca
    35521 tatggcgacc ctgcgtgaaa ccgaagataa gctgtacacg gattttcaaa ccgtactcga
    35581 agggcgcaaa gagctgactc tgaaccggga acgcgccgag tatgtgttta acaacctcta
    35641 cattcctgat gcgcaagagt atcgccatca tattatccgc gcagacacct tccatcttag
    35701 tgccgtgaac tggtcaaaca tcatgatgct gggcgctatc ggcctggtgt tctggatggc
    35761 gaacagtctc ggttgggctg ataccaacgt tgccgcgacc tattcgttga cgcttttatt
    35821 cctgcgtacg ccgctgcttt cggcggttgg cgcattgccg acgctgctga cggcgcaggt
    35881 ggcgtttaac aagctgaaca aattcgcgct cgcgcctttc aaagcagagt ttccgcgccc
    35941 gcaggcgttt cccaactggc aaacgttgga gctgcgtaac gtgacgtttg cttatcagga
    36001 taacgcgttt tccgttgggc cgattaacct caccatcaaa cgtggtgaac tgctgtttct
    36061 gattggtggc aacggtagcg gaaaatcgac gctggcgatg ttgctgacgg gcttgtatca
    36121 gccacaaagc ggcgaaatct tgctggatgg caaacctgtc agcggcgaac aaccggaaga
    36181 ttatcgcaaa ctgttttcgg cagtgtttac cgatgtctgg ctgtttgatc aactgctggg
    36241 gccggagggg caacccgcta acccgcaact ggttgagaag tggctggtgc agctgaaaat
    36301 ggctcataag cttgagttaa gcaacgggcg tattgttaac ctgaagttat caaaagggca
    36361 gaaaaaacgc gtggcgctgt tgctggcgct ggcagaagaa cgcgatatta tcctgctgga
    36421 tgaatgggcg gcggatcagg acccacactt ccgtcgtgag ttttatcagg tgctgctgcc
    36481 gctgatgcag gagatgggta aaaccatatt cgccatcagt catgatgatc attactttat
    36541 ccacgctgac cgcctgctgg aaatgcgcaa cgggcaactt agcgagctga caggcgaaga
    36601 gcgcgatgct gcttcgcgtg atgccgttgc ccggacggca taatattacg acgctgacct
    36661 ggcggactgc tgccgccagg tcaatattct aaaaatacct tcgtttctaa taaccctata
    36721 attaatcaac gaaattataa tgtttctaaa attagaatat aatttataaa cattatttaa
    36781 atgttgttac ttaagtgtta ccgttgatgc cgcgcaaatc tcacctgcaa taaagcgact
    36841 aaaagtaagg cattaacaag atgaaaaaag tgactgccat gctcttctcg atggccgtgg
    36901 ggcttaatgc cgtttcgatg gcggcaaaag cgaaagcgtc cgaggagcag gaaactgatg
    36961 tactgttgat tggcggcggc attatgagcg ccacgttggg gacctattta cgcgagctgg
    37021 agcctgaatg gtcgatgacc atggtggagc gcctggaagg tgtcgcgcag gagagttcga
    37081 acggctggaa caacgccgga accgggcatt ctgcactgat ggaactcaat tacaccccgc
    37141 aaaatgccga cggcagcatc agtattgaaa aagcagtcgc cattaacgaa gcatttcaga
    37201 tttcccgcca gttctgggcg caccaggtcg agcgcggtgt gctgcgtact ccgcgttcat
    37261 ttatcaatac cgttccgcat atgagctttg tctggggcga ggataacgtt aatttcctgc
    37321 gcgcccgtta cgccgcgttg caacaaagct cgctgtttcg cggtatgcgt tactctgaag
    37381 atcacgcgca gatcaaagag tgggcaccgt tagtgatgga agggcgcgat ccgcaacaga
    37441 aagtagcggc aacacgcaca gaaattggca ctgatgtgaa ctatggcgag atcacccgcc
    37501 agttgatcgc ctccttacag aagaaatcta acttctcgct gcaactcagc agcgaagtcc
    37561 gcgccctaaa gcgtaatgac gataacagct ggaccgtcac cgttgccgat ctgaaaaacg
    37621 gcactgcaca gaacatccgc gccaaatttg tctttatcgg cgcgggcggt gcggcgctga
    37681 aactgttaca ggaatcgggg attccggagg cgaaagacta cgccggtttc ccggtgggcg
    37741 gacagttcct tgtttcggaa aacccggacg tggttaatca ccatctggcg aaggtttacg
    37801 gtaaagcatc cgttggcgca ccaccgatgt cggttccgca tatcgatacc cgtgttcttg
    37861 acggtaaacg cgtagtgctg tttgggccat ttgccacctt ctcaaccaaa ttcctcaaaa
    37921 acggttcatt gtgggatctg atgagttcca ccaccacctc taacgtgatg ccgatgatgc
    37981 acgttgggct ggataatttc gatctggtga aatatctggt gagtcaggtg atgttgagtg
    38041 aagaggatcg ttttgaagcg ttgaaagagt actatccgca ggcgaaaaaa gaggactggc
    38101 gtttgtggca agcggggcag cgcgtgcaga ttatcaagcg tgatgccgat aaaggtggcg
    38161 tactgcgtct gggtactgaa gtcgtcagtg accagcaagg gaccattgcc gcgctcctgg
    38221 gggcatcgcc aggagcgtca accgccgcgc cgattatgtt gaatctgctg gaaaaagtat
    38281 ttggcgatcg cgtttccagc ccgcaatggc aggctacgtt gaaagcgatc gttccgtctt
    38341 atggacgcaa gctgaacggt gatgtagcgg caacagaacg cgagttgcag tacaccagcg
    38401 aagtgctggg gctgaaatac gacaagccgc aggcagcaga tagtacgccg aaaccgcagt
    38461 tgaaaccgca acccgttcaa aaagaagtgg cggatattgc gttgtaatga tgtgccacat
    38521 ccggcatggt ttgccggatg tggcgtatgc tgataagacg cttcagcgtc gcatcaggca
    38581 accggctcgg gcgttagatg tcggatgcgg cgtaaccgcc ttattcggcc tacaggttgc
    38641 agcacttcac agcagtttag cggacaaccg cgttgtcaat tttctcttcc gccttccaga
    38701 cacggtactt cacatccaca ttgtctggcg tatacaccac gatcggcagc ttgctgttgt
    38761 aacgcagcat tccagcatcg cccagatacg cggtgacaaa tttcttctct ttcttgccat
    38821 ccgggcaggc catcatcgtg gaaaccggcg agctaacttt atcaaagaca taataatcat
    38881 agccccagcc ttccagcgtt ttgctttcca gcttcccgcc gagacgatgc aaattgcagt
    38941 cgacttccag cgtctgaccg attaacagtt ctactttcag ggtagattca tcttcttgcg
    39001 gagttaactg aatcacctga cgcttcatgc ctttttcagc ttgtggataa ggtgcgattt
    39061 tttccagtgg ctggacgctt tctgccgccc aggcggaagt ggtggcgaaa gcggcaaaca
    39121 atactgcagg tagaatggtc ttcatttttt tgctttcctt cttcatttat aggttttcac
    39181 cgatgatgtt gctgaaggtt aacatattca tcagatttat taatttgctt tatgaataac
    39241 ttaaagaaca ttcagataat tttacctgct cttttagtgt taaattctga tgagaaaaca
    39301 aaccagcaga a
//
LOCUS       DAPHCV010000037        38383 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.037, whole genome shotgun sequence.
ACCESSION   DAPHCV010000037 DAPHCV010000000
VERSION     DAPHCV010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.037"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            85..1740
                     /gene="aslA"
                     /locus_tag="RH033_001884"
     CDS             85..1740
                     /gene="aslA"
                     /locus_tag="RH033_001884"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HDV2991080.1"
                     /translation="MEFSFSPKLLVVAVAAALPLIASAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKETQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIQKLPFSKDDVHAVRGGEQEAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDIFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLAAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(1842..3077)
                     /locus_tag="RH033_001885"
     CDS             complement(1842..3077)
                     /locus_tag="RH033_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDV2991081.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASETQNEVAFTWQGGEPTLLGLDFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSALQPLQIYDFLCDAGVEFIQFIPVVERLADETAVHAGLKLH
                     APGDIQGELTEWSVCPQEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMLQQMIAEMIDSPQQQAFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(3224..3300)
                     /locus_tag="RH033_001886"
     tRNA            complement(3224..3300)
                     /locus_tag="RH033_001886"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3264..3266),aa:Pro,seq:tgg)
     gene            complement(3343..3429)
                     /locus_tag="RH033_001887"
     tRNA            complement(3343..3429)
                     /locus_tag="RH033_001887"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3393..3395),aa:Leu,seq:cag)
     gene            complement(3450..3525)
                     /locus_tag="RH033_001888"
     tRNA            complement(3450..3525)
                     /locus_tag="RH033_001888"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3490..3492),aa:His,seq:gtg)
     gene            complement(3584..3660)
                     /locus_tag="RH033_001889"
     tRNA            complement(3584..3660)
                     /locus_tag="RH033_001889"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3624..3626),aa:Arg,seq:ccg)
     gene            complement(3763..5148)
                     /locus_tag="RH033_001890"
     CDS             complement(3763..5148)
                     /locus_tag="RH033_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV2991082.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLVAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPTAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLTVTAIYKVFGL
                     NRHGKAHKLEK"
     gene            complement(5339..6079)
                     /gene="rffM"
                     /locus_tag="RH033_001891"
     CDS             complement(5339..6079)
                     /gene="rffM"
                     /locus_tag="RH033_001891"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HDV2991083.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKSEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(6082..7434)
                     /gene="wzyE"
                     /locus_tag="RH033_001892"
     CDS             complement(6082..7434)
                     /gene="wzyE"
                     /locus_tag="RH033_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HDV2991084.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADAPR
                     RPLFTMNRVETNLTWVILMVIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(7431..8510)
                     /gene="rffT"
                     /locus_tag="RH033_001893"
                     /gene_synonym="wecF"
     CDS             complement(7431..8510)
                     /gene="rffT"
                     /locus_tag="RH033_001893"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HDV2991085.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCLALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFYWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDSSLNTMVNDRQREVKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLTLLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(8507..9757)
                     /gene="wzxE"
                     /locus_tag="RH033_001894"
     CDS             complement(8507..9757)
                     /gene="wzxE"
                     /locus_tag="RH033_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HDV2991086.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAVIMLIKRGAIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(9759..10889)
                     /gene="rffA"
                     /locus_tag="RH033_001895"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(9759..10889)
                     /gene="rffA"
                     /locus_tag="RH033_001895"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HDV2991087.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(10894..11568)
                     /gene="rffC"
                     /locus_tag="RH033_001896"
                     /gene_synonym="wecD"
     CDS             complement(10894..11568)
                     /gene="rffC"
                     /locus_tag="RH033_001896"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HDV2991088.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRISPEAPLLTPDALAPWSRV
                     QAKIAASNTDELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRSASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(11546..12427)
                     /gene="rfbA"
                     /locus_tag="RH033_001897"
     CDS             complement(11546..12427)
                     /gene="rfbA"
                     /locus_tag="RH033_001897"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDV2991089.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTQGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(12446..13513)
                     /gene="rffG"
                     /locus_tag="RH033_001898"
     CDS             complement(12446..13513)
                     /gene="rffG"
                     /locus_tag="RH033_001898"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDV2991090.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFVETNI
                     VGTYTLLEAARAYWNALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVDDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWCKQVQDGSYQGERLGLKG"
     gene            complement(13513..14775)
                     /gene="wecC"
                     /locus_tag="RH033_001899"
     CDS             complement(13513..14775)
                     /gene="wecC"
                     /locus_tag="RH033_001899"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HDV2991091.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGELADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(14772..15902)
                     /gene="wecB"
                     /locus_tag="RH033_001900"
     CDS             complement(14772..15902)
                     /gene="wecB"
                     /locus_tag="RH033_001900"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDV2991092.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(15958..17004)
                     /gene="wzzE"
                     /locus_tag="RH033_001901"
     CDS             complement(15958..17004)
                     /gene="wzzE"
                     /locus_tag="RH033_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HDV2991093.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIVFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(17016..18119)
                     /gene="wecA"
                     /locus_tag="RH033_001902"
     CDS             complement(17016..18119)
                     /gene="wecA"
                     /locus_tag="RH033_001902"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HDV2991094.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(18359..19618)
                     /gene="rho"
                     /locus_tag="RH033_001903"
     CDS             complement(18359..19618)
                     /gene="rho"
                     /locus_tag="RH033_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDV2991095.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(19703..19804)
                     /gene="rhoL"
                     /locus_tag="RH033_001904"
     CDS             complement(19703..19804)
                     /gene="rhoL"
                     /locus_tag="RH033_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HDV2991096.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(19945..20274)
                     /gene="trxA"
                     /locus_tag="RH033_001905"
     CDS             complement(19945..20274)
                     /gene="trxA"
                     /locus_tag="RH033_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HDV2991097.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            20405..21670
                     /gene="rhlB"
                     /locus_tag="RH033_001906"
     CDS             20405..21670
                     /gene="rhlB"
                     /locus_tag="RH033_001906"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HDV2991098.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAQATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            21804..23288
                     /gene="gppA"
                     /locus_tag="RH033_001907"
     CDS             21804..23288
                     /gene="gppA"
                     /locus_tag="RH033_001907"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HDV2991099.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLAQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLTVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFYDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIDQESQWQSYVHWPLEVH"
     gene            complement(23335..25356)
                     /gene="rep"
                     /locus_tag="RH033_001908"
     CDS             complement(23335..25356)
                     /gene="rep"
                     /locus_tag="RH033_001908"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HDV2991100.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            25443..25724
                     /gene="ppiC"
                     /locus_tag="RH033_001909"
     CDS             25443..25724
                     /gene="ppiC"
                     /locus_tag="RH033_001909"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HDV2991101.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(25770..27245)
                     /gene="ilvC"
                     /locus_tag="RH033_001910"
     CDS             complement(25770..27245)
                     /gene="ilvC"
                     /locus_tag="RH033_001910"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDV2991102.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            27395..28288
                     /gene="ilvY"
                     /locus_tag="RH033_001911"
     CDS             27395..28288
                     /gene="ilvY"
                     /locus_tag="RH033_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HDV2991103.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLSTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAIVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(28340..29884)
                     /gene="ilvA"
                     /locus_tag="RH033_001912"
     CDS             complement(28340..29884)
                     /gene="ilvA"
                     /locus_tag="RH033_001912"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HDV2991104.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFEIRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(29887..31737)
                     /gene="ilvD"
                     /locus_tag="RH033_001913"
     CDS             complement(29887..31737)
                     /gene="ilvD"
                     /locus_tag="RH033_001913"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDV2991105.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDDSALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDMIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYGSLATSADKGAVRDKSKLGG"
     gene            complement(31802..32731)
                     /gene="ilvE"
                     /locus_tag="RH033_001914"
     CDS             complement(31802..32731)
                     /gene="ilvE"
                     /locus_tag="RH033_001914"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HDV2991106.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(32751..33014)
                     /gene="ilvM"
                     /locus_tag="RH033_001915"
     CDS             complement(32751..33014)
                     /gene="ilvM"
                     /locus_tag="RH033_001915"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HDV2991107.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(33011..34657)
                     /locus_tag="RH033_001916"
     CDS             complement(33011..34657)
                     /locus_tag="RH033_001916"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HDV2991108.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGMEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDILGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVSALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQDDLNALLPALQQPLNIDDWQQHC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKSADCIVTTDVGQHQMWAAQHIVHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITHKDQVEAAL
                     DTMLNSDGPYLLHVSIDEFENVWPLVPPGASNSEMLEKLS"
     gene            complement(34660..34710)
                     /locus_tag="RH033_001917"
     CDS             complement(34660..34710)
                     /locus_tag="RH033_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2991109.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(34797..34895)
                     /gene="ilvL"
                     /locus_tag="RH033_001918"
     CDS             complement(34797..34895)
                     /gene="ilvL"
                     /locus_tag="RH033_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HDV2991110.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            35248..36768
                     /locus_tag="RH033_001919"
     CDS             35248..36768
                     /locus_tag="RH033_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDV2991111.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTNAVSRAQQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(36793..37131)
                     /gene="maoP"
                     /locus_tag="RH033_001920"
     CDS             complement(36793..37131)
                     /gene="maoP"
                     /locus_tag="RH033_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HDV2991112.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            37250..38089
                     /gene="hdfR"
                     /locus_tag="RH033_001921"
     CDS             37250..38089
                     /gene="hdfR"
                     /locus_tag="RH033_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HDV2991113.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(38184..38259)
                     /locus_tag="RH033_001922"
     tRNA            complement(38184..38259)
                     /locus_tag="RH033_001922"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38224..38226),aa:Trp,seq:cca)
     gene            complement(38268..38344)
                     /locus_tag="RH033_001923"
     tRNA            complement(38268..38344)
                     /locus_tag="RH033_001923"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38308..38310),aa:Asp,seq:gtc)
BASE COUNT         9386 a        10510 c         9696 g         8791 t
ORIGIN      
        1 aatcatcctg tcagggagag ggatctcaat tatcaatgct taattacgtc atcattttga
       61 cgacatgaaa ccggagcatt ttgcatggaa ttttcgtttt cacccaaact tcttgttgtt
      121 gctgtcgccg cagctcttcc tctcatagcc agcgcagcag ataccccgtc aaccgccacc
      181 gcacgcaaag gctttgccgg atacgatcac ccaaaccagt atctggttaa accggcgacc
      241 actattgccg acaacatgat gccggtgatg cagcatcctg cacaagacaa agaaacccag
      301 cagaagctgg cagaacttga gaaaaaaacc ggtaagaaac cgaacgtggt tgttttcttg
      361 ctggatgatg tgggctggat ggatgttggc tttaacggcg gcggcgtggc ggtgggtaac
      421 cctacaccag atatcgacgc cgttgccagc caggggctga ttttaacttc ggcgtattct
      481 caaccgagtt cttccccaac ccgcgccacg attcttaccg gacaatactc catccaccac
      541 ggcattctga tgccgccaat gtacgggcaa ccgggcggtc tgcaagggtt aaccacgctg
      601 ccgcagttgc tgcatgatca gggctacgtc acccaggcca tcgggaaatg gcatatgggg
      661 gaaaacaaag agtcgcagcc gcagaacgtt ggctttgatg atttccgcgg ctttaactcg
      721 gtgtccgata tgtataccga atggcgtgat gttcatgtca acccggaagt cgccctgagc
      781 ccggatcgtt ctgaatacat ccaaaaactg ccgttcagca aagatgacgt tcatgccgtg
      841 cgcggcggtg aacaggaagc cattgccgac attacgccga aatatatgga agacctggat
      901 caacgctgga tggaatatgg cgttaagttc ctcgacaaga tggcgaagag cgataagcct
      961 ttcttcctct actacggcac tcgcggctgt cacttcgata actacccaaa tgcgaaatat
     1021 gcgggtagct ctccggcgcg cacctcttac ggcgactgca tggtggagat gaacgatatc
     1081 ttcgccaatc tgtataaagc actggagaaa aacggtcagc ttgataacac gctgattgtg
     1141 ttcacctctg ataacggtcc ggaagccgaa gttccgccgc acggtcgcac accgttccgc
     1201 ggtgcgaaag gttctacatg ggaaggcggc gttcgtgtgc caaccttcgt ttactggaaa
     1261 ggcatgatcc aaccgcgtaa atctgacggc attgtcgatc tggcggatct cttccctacg
     1321 gcgctggatc tggcggggca tcctggggcg aaagtagcga atttagtacc gaaaaccacc
     1381 tttatcgatg gtgtggacca gacatctttc ttcctgggaa caaatggtca gtctaaccgt
     1441 aaggccgagc actacttcct caacggtaaa ctcgctgctg tgcgtatgga tgagttcaag
     1501 tatcacgtcc tgattcagca accttacgcg tatacccaga gcgggtatca gggtggattc
     1561 accggcacag taatgcaaac ggcgggatcg tcggtgttta acctgtacac cgatccgcag
     1621 gaaagcgact ccatcggcgt gcgccatatc ccaatgggtg taccgctaca gaccgaaatg
     1681 cacgcgtata tggagatcct gaaaaaatat ccaccacgcg cgcagattaa atctgattaa
     1741 gccggcgcta tcgctacatc gggccacact gaccttgccg gatgcggcgt gaacgcctta
     1801 tccggcaatc aaccgcaggc ggccacaaaa tggccgcttt tttacttatt caccaccatc
     1861 aaatgcgcct gcataatgtc gctggccgga cgaccgtgcg ccagcaaatc agccattgct
     1921 ttaagatatg gcggtagatg gcggaaataa cgctgatacc cggcacacaa ataattcagc
     1981 ccgggtttgc cgctggcatc gagcataaag cggtgtttcg ggcagcctcc ccagcacgct
     2041 tttaacacgt tgcaactgcg acactgtgcc ggtaactgct tgaatttatc ttcaccaaat
     2101 gcctgctgtt gcggggaatc aatcatttct gcaatcatct gctgaagcat attccctagt
     2161 cgatactgcg gataaacata gtgatcgcag gcataaacgt caccgttgtg ctcaacaatc
     2221 accgagcgcc cacaggttgg ctgatgatgg caaaccgcgc ccggtgcgcc gacaaaattg
     2281 gcgaacgccc attcgatatt cattacaaaa atcttgccga cgtcgcgctt aatccagtgg
     2341 tcgaaaatcg ccaccagaaa ctcaccgaac tcttgcgggc acaccgacca ttccgtcagt
     2401 tcgccctgaa tatcgccggg agcatgtagc ttaagtccag catgaacagc cgtttcatca
     2461 gccaggcgtt cgaccacagg aataaactgg ataaactcaa cgcctgcatc acacagaaaa
     2521 tcatagattt gcaaaggttg taacgcactg gtgcgattta cgcagaccag cacgttatag
     2581 tcgacatgat gtttttgcag gagcgtcagg gcacgcatca ccagcttatg cgttggtctg
     2641 ccgcctttgg tcacgcgata ttgattgtgg atctcagccg gaccatccag cgataaccca
     2701 acaagaaaat gattttctgc cagaaatgca caccattcgt catcaagcag cacgccgtta
     2761 gtctggaagc tgttacttat cttcctgcca gcaccatatt tcgcctgtag cgccacggca
     2821 cggcggtaaa aatccagccc gagtaacgtt ggttcgccgc cctgccaggt aaaagccact
     2881 tcgttttggg tttcgctggc agcgatatag tgacggacat acgcttccag cgtgtcatcg
     2941 tccatatgcg tgactggctt ttcgcggtaa agggattgtt tttcgagata aaaacagtag
     3001 tcacagttca gattacaatc ggaaccgctc ggtttcgcca tcacatgaaa agcacgcgtt
     3061 ggaacctgtt gcagcatggt cgctccttgc ctgaagtcat ccttcaataa gttaagggcg
     3121 aaatcatacc gtggcgcaat gcgttattac gggcgtttat tgcgaaggga aagtgacgaa
     3181 atgcacagaa aacaaaaaac cccgccgaag cggggttcaa aattggtcgg cgagagagga
     3241 ttcgaacctc cgacccactg gtcccaaacc agttgcgcta ccaagctgcg ctactcgccg
     3301 aaatactgct ttttgaattt ttagttcaat tctttaaagt cgtggtgcga ggggggggac
     3361 ttgaaccccc acgtccgtaa ggacactaac acctgaagct agcgcgtcta ccaattccgc
     3421 caccttcgca ttgtcacaac ttctaataat ggggtggcta atgggattcg aacccacgac
     3481 aactggaatc acaatccagg gctctaccaa ctgagctata gccaccacta caaatcttgt
     3541 tacgcggtat tactaccacc gcagctcaag cgccgggact aaatggcgcg cccgacagga
     3601 ttcgaacctg agacctctgc ctccggaggg cagcgctcta tccagctgag ctacgggcgc
     3661 ttagcgccgt tgcgggggtg gataatacgg acttcccacc cctctgtcta gtcccttttt
     3721 aaataaattg cgcgtttggt tacggtttgt gcgctttgct gcttatttct ccagtttatg
     3781 cgctttaccg tggcgattaa gcccaaaaac tttataaatc gccgtcaccg tcagcataaa
     3841 gatgatgccg acaaacagcg acatacgcgt atcttcatta aagtacatgc cgatcaaaac
     3901 gcaaatcagg aatgccattg ttacgtagtt ggcccacggg aacaggattg agcggaacgg
     3961 atggctggca attgccgctt tatgcgcacg acgaaaacgc agctggctaa tcaatatcac
     4021 aaaccatggc accatccccg gaagcacgct ggcactgtag acgtagacaa acacacgctg
     4081 cggattggga atgatgtagt tcaggcatga gccaattaac aggatagcaa tagatactgc
     4141 cacacccgca accggtacgc cgtgacggga aactttcgcc atcgccgtcg gtaactgacg
     4201 gtttttcgcc aatgcgtaga gcatacgtcc gcaactgtac atgccgctgt tacagccaga
     4261 gagcgcagcc gtcagcacca caaagttgat aatgcccgcc gctgcggtaa tgccgatttt
     4321 ggcgaaagtc agtacgaacg ggctgccgtt actgcctatt tcattccacg ggaagatggt
     4381 gacgataacg aaaatcgcgc ctacgtagaa aatcaggata cgccacaaca ctttgcctac
     4441 ggcactgcgc agcgtcacct gcggattctt cgcttcaccg gcagtaatgc caatcagctc
     4501 cacgccctgg taggacgcca ccacaataca caatgcggtc aggaaccctt tccagccacc
     4561 cgcaaagaaa ccgccatgct ctgtgagatt gctaaaacca atcgactgcc cgccattgcc
     4621 aaagccaaag aaaatcacgc ccaggccaat gacaatcatc acgataatcg tggtgacttt
     4681 gatcatcgcg aaccagaact cgatttcgcc gtacaacctc accgcaacca gattcgccaa
     4741 cgccaccagc gccaccgcga tcaatgcggg tatccactgc gccatctccg ggaaccagaa
     4801 ctggacataa acgccaatgg cggtgatttc tgagatcccc accgccatcc acataaacca
     4861 gtaagaccag gcggtgagat aaccaaagaa cgggctcata taacgatgcg cgtaaacggc
     4921 gaacgaaccg gtaaccggtt cgaggaacaa catttcgccc attgaacgca tgatgaaaaa
     4981 gacgaacagc ccggcgatga tataggccaa caatacggat ggcccggccc atttcagggt
     5041 actggcggcc cccataaaca ggccgacgcc aatggtgccc cccagggcga tgagttcgat
     5101 atgtcgagct tccagcccac gctgtagctc tggtttgtta tctgccataa atcctcgtgt
     5161 tgtgtttgca tgctttccgg tgttaccggt tatcgttatg ggtacatcga gtgttgcgaa
     5221 tgttttcgta attcaggaga aatggcaaat aaagcattaa aaatttgaat gctttgtgta
     5281 ataaaaaagc agacaggcga cgaagtgacc acttcgtcgc tttacaaaga gaggaaaatc
     5341 agaggttgcc ggtgtaatgc cagcgtaaat aacgcagcaa acgaagctga cgcttaatgc
     5401 ggctcggctg cgaaagcagg cggtagagcc actccagccc cagcgtttgc cagattttcg
     5461 gtgcgcgttt tacgtgaccg gtgaaaacat cgtaggtccc gccaacgccc atatacagcg
     5521 catctggatg caccagacgg cagtcgcgca tgaagatctc ctgctttggc gatcccatcg
     5581 caacggtgac gatttgcgct ccgctggcat gaatgcgttc aaacagcgcc tgacgctgct
     5641 cgggtttaaa ataaccatcc tggctgccaa cgatattcac attccactgg ttgcgcagtt
     5701 tagcttcagt ttgcgccagc acttcagatt taccgcccac aagaaatacc ggtgtccctt
     5761 ctttgcctgc gcgcgccatc agctcttccc agagatcggc cccagcaacg cgggaaacct
     5821 gcgcctgcgg gtacttttta cgtactgaac gtacaacgct gatgccatcc gcatatttaa
     5881 attcggcagc gttaattaac tccctgacct cggcgttatc ttcaatggtc agcatttttt
     5941 cggcattaat ggcaaccagc gttccctgct taagctgccc gtcagcgaac agataatcga
     6001 gggcatgctg catatcacgc caaccaatca actgtaagcc acgcagcgta taggtcggtg
     6061 ccgtggtgtt gttattcatt gttatccttc aacctgcgtc cggagcgatg attttgttcg
     6121 tttatgaatg agtccggcgc tttcaaaaag ccagtacaac agttttgcga tcatcagaca
     6181 tgcgccgaag accacgataa aaaagaccac gcgtgagaca aacgaatcca gcccttcacg
     6241 cgccagcacg atcatattga agatcgcccc gaagcagaaa ctgtgcaata tcgcagcctt
     6301 atagcgatta gtctcgcggt tgcccagctc atacagccag tcgaaccatt tgatgatcag
     6361 tccaacaaca atcgccccga gcgggatgaa caacgcgccg cccatcacca ccagtgagcc
     6421 tataagcgta ggcgagatcg ccagtccgga gtggttattc agtacttccc aggtaaagta
     6481 gttggcggag ttcagcacca tactcgggcg acccggccac agccaggaag ggataaagac
     6541 atagaaatcg cggacaattg gagccaggcc ctggaagtcg atgttgtcgt agttctgcaa
     6601 cagcaacgcc agattctccc acggcgagaa ggtgtcgcga gtgagataga gaaacgtata
     6661 gaacgcttca tcgccgctca cattcattcc atagcgtttt agcgccagcc agaacatgcc
     6721 cacaatcccc agcacgcccg ccgccgccag catccacaac gaaatccagc cgcgaataat
     6781 gccaataaac aggaagatag cgaatgcgat gatgatattg gcgcgagtgc cgccgacaat
     6841 catgtaagtc aacaagccaa aggcaaccgt gctgacgagg aaaaacagcc acgctttgct
     6901 atcctggcgc agaaaataaa ccaccagcat cgccgggatg aaaaagtaaa agaagcgttt
     6961 taatgccacg ccggagactt cgctggaaaa gatctgactg taagaattaa gccggaacag
     7021 caaaaagccg ttgtgcatga agaagatgcc gacgctcacc agagcgatga ccatcaggat
     7081 cacccacgtg agattggtct ccacgcggtt catggtaaac agcggacggc gcggtgcatc
     7141 agcaacgcgt ttgcgtagac gggttttgta ggtgacatag taaaccgcgt agaagcagcc
     7201 tgcagaaagc aacgcctgca acaagatttc tggcggcgcg acaccaacat caaagcgaaa
     7261 taccagcacg ctggtcagcg gaaagccgaa gaaaaaggtg agcaaaaaca gcaatgaaaa
     7321 gaagacattg aagttaaagc gcacacggcg aaactcaaac caggtcagcg tggcaataaa
     7381 cagcgtgcag agcagccaga caacaaacag gccactgaat tgcagcagac tcatgcgacc
     7441 tccccggcgg caatcgccaa cgcccgctgc cagccttgta gatagttagg gctaaagaag
     7501 gcaatggtgt ttttatccac cgacgccaac tggcgctgcg cttcacgcac aatatcctcg
     7561 ttgagatcat cggtagtaaa cagtactggc aaatgctgtt ccgtcatatc ctgccagaac
     7621 ggattttccc ggttaagcac acaaggaatg cccgcctgaa tcagtaagca aagcgtacca
     7681 atgccctgct ggcgggcaaa aataaagtaa ccgagatcgc actgacgaag tagcgtcaga
     7741 taggcgtcaa attccagttt ttcgctcaga acttgcagat tttcttcgct gaataactcc
     7801 agtcccgcct gacgaacttc ctcgatgtac gcttcgttat taggcggata tcccatcggc
     7861 accaccactt ttaccgtatc gccaaattgc tgatgaacgg cgcgcaaggc agcaatatgc
     7921 tcattgctgc ggtcaccgga gttccccacc agaatggtca tttttacttc acgttgccga
     7981 tcgttcacca tcgtattaag agaagagtcc atccgcgtcg ggaagtacag cagttcgccc
     8041 cgcacctttg ggtgcgtttt ggcaaaaaag ctcaaatcac cgcgggtggc aaatacacag
     8101 ccgactcgct tttgcgccag gcgacgtagt gggtaaaaaa gcttatatct caagccactg
     8161 gaaagttcgt acaggtctgc cccccagata tgccagtaaa actggctggg cttaatgcca
     8221 ccactcagca gagccagcca cagtgtggga ttgaactgac cgtggaagaa aaaacgctgc
     8281 tgacggttag cttttgcttt cgcgatgacc gcttccgcca gcgatttttt cccagggaaa
     8341 aattgcacag aaagcgccag acagctatca cttaagccgt cgtccttgcc aacaaccata
     8401 aactcgcgcg cgtgctcgct cgtcgcggcc agcgcgtcat tgaaaaaccg caaaacggtt
     8461 cggttatggt gagggatatc cgatcccagt acgtgaatca gtacagtcat gcccgcctac
     8521 gccagagtaa aaacacgcca caacaaagag aaaaatagac gatataggtt gccatatagg
     8581 cctgcgccgc gcccagtgca ccatgcgcag ggattagcca gtgggcaaat accatcaata
     8641 aagtgaactg gctgacttcc gccagaatat aaaaccgcag tgacgctttg gcgatcacca
     8701 gataaccaaa gacataagcg cccactttta acacatcacc cactaactgc caggcaaaga
     8761 gatcgcgcat agcggtaaat ttattcgaca acagcagcca gatagcaaaa tcacgcagca
     8821 accagacggt aaaactcgcc gccgccactg ccggtaagac gaatttcagc gatttaacca
     8881 cttcccgggt gatatcgcgc ttttccgtta gccgcgacaa cgtgggcagc aaatatacgc
     8941 tgaacgatgc cgtaataaat tgcaggtagg catcggaaat actgctcacc ccttgccaga
     9001 tccccacctc atcccagcta tactgcgccg ccagcagttt acgcatcatg atgtaagcaa
     9061 caggcaaggt caccgacgta atcaacgcca tgagcgtaaa tttgctcaac tgccctgcca
     9121 ggccgttatc ccagctgggt ttcagatagc ttaacgggat tgcaccacgc ttaattagca
     9181 tgatgacggc aggaatcacc accagcgcgg gaatcaacgc cagccccagc aacgcccctt
     9241 cataaccgcc caaacggtaa ctgacgtagt acgcgagaac gccaatcaag ctgccgacaa
     9301 tcagggataa cgcattacct gcggcatcgc gaaagccttt catcagcgcc agtaacaggt
     9361 tgccccaggc gatccccatt tgcaccagcg ccactaaacg caccagcccc tgatagtcgg
     9421 tattaccaaa caatccctgg ctgattggcg cagctgccag cacaaaaacc agcgccatca
     9481 gcgtagagaa accaagtacc atcgctgatg aagtgccgac cacgcggcgc agctgctgcg
     9541 gattatcatg gtactgagca acgtatttgg ttacgccatt aaagatgccc gccccggcaa
     9601 gcacgccgag cacggtaatc aactggcgga aatttgccgc cagcccaagc cccgccggac
     9661 caaatgacac cgccagcaac ttaccgacca gtaacccggc accaatcttg accagtgtac
     9721 tggccgccgt ccacaaggac gcttttgcca acgacatatc aggagaagta gttcaacaaa
     9781 gtcgcaatta ccgtacgctg attgacgggc gacaggttgt agaacagcgg caggcgcagc
     9841 aggcgctcgc tctctttggt ggtgtagcga tcttcaccgt ggaactcacc aaagcgttcc
     9901 cccgcagggc aaccgtgcag cggaatgtaa tggaacaccg ccatgatttc cgcttctttc
     9961 agaaagttaa tcaacgcgct ccggtcatca atatcccgca gtttaatgta gaacatatgc
    10021 gcgttctgca cgcagccatc gggaatcgac ggcagctcga tacgcccggc tttcgccaga
    10081 ggtgctaacg catcgtagta gttttgccac agcgccagac gttgctgatt gatacgatcc
    10141 gctgcttcca gttgcgccca caggtacgca gcttgcagat cggacatcaa atagctggag
    10201 ccaatatcgc gccaggtata tttatcgacc tgaccacgga agaactggct gcggtttgtg
    10261 cctttttcac ggatgatctc ggctcgttcg atcaacgctt tatcgttaat cagcgtcgcg
    10321 ccgccttcac cgcccgccgt gtagtttttg gtttcatgga agctaaagca gccaatatga
    10381 ccaatggttc ccagtgcacg ccctttgtaa gtggacatca cgccctgagc agcatcttcc
    10441 accacaaaca gattatgctt tttcgccaac gccataatgg tgtccatttc gcaggccaca
    10501 cccgcgtaat ggaccggcac gataacgcgc gttttgtcgg tgatcgccgc ttcaatcagc
    10561 gtttcgtcga tgttcatggt gtccgggcga acatccacaa aaacgatttt tgcgccacgc
    10621 agcacaaagg cattggcggt ggagacaaag gtgtagctcg gcatgatcac ttcatcgcca
    10681 ggctggatat cgagcagcag cgccgccatc tccagcgaag cggtgcagga tggcgtcagt
    10741 aacactttgg cgctgccaaa acgttgctcc agccactgct ggcagcgacg ggtaaaaccg
    10801 ccatcgccac acagtttgcc actacccatt gccgactgca tatagtcgag ttcggttccc
    10861 accaccggcg gtgcgttaaa tggaatcatg tgatcacctg tataaccagt acgcggtgct
    10921 ttctacattc gcaccacttt gtatgtatcg ttttagcgcg gcggtgttgc ccatttgggt
    10981 cgccacccgc aaagttgttt taccgcgagc atacgcccag tttagcgctg tttgcatcag
    11041 ctcagcacct gcaccgcgtc cagccagcag gccaattcgc gcatctgtcg cattgagttc
    11101 ccgtaaagag acatagccac gaatatcgcc ggacgccgaa cgtaaaatga ggcattgatg
    11161 atcaaaggtg ccgcgcacgg cattttcaat ccactgtgca taaaagcgac cgctggcgtc
    11221 aggcgcatac cacggcgcac gaaaacggct ttgcgcaaat gcggcgctgg ctaactgacg
    11281 taatgcggga atatcggtct cttgtgccac tacagcacca ctatcactga cattgtttac
    11341 gggtagcgcc aaatcaactt caccttctac cagggagaat cccagctgtt gcaaggcatc
    11401 cagttcatcc gtatttgatg ccgcaatttt ggcctgcacc cgtgaccacg gcgctaaggc
    11461 gtctggcgtc aggagcggtg cttcaggact aatgcgcaca atggcgctgt taacaccaaa
    11521 gaaggcgttt tcccaggtta gtggctcaat actggcgcgg acgggcacga agtaactcca
    11581 gcagatattg gccgtagcca gttttcgcca gcgagctggc ggcgcgcttc acgccttcgt
    11641 catcaagcca gccgttacgc caggcgatct cttccaggca ggcaatctta aagccctggc
    11701 gtttttccac cgtctgtaca aaggtgctgg cttcaatcag gctgtcgtga gtgccagtgt
    11761 ccagccaggc aaatccgcgc ccgagcagtt caacggtcag gttgcccgcc tcgaggtaca
    11821 tctggttgat ggaggtaatc tccagttcac cacgctccga cggcttcacc tgctttgcgt
    11881 actccacgac tttactgtcg tagaaataaa gcccggtcac cgcccagttt gacttcggct
    11941 gttttggctt ttcttccagc gagatagcgc ggaaattgtc gtcaaactcc acgacgccaa
    12001 agcgttccgg atccatcacc tgatagccaa ataccgtcgc gccttgcgtg cgcgccgcaa
    12061 catgacgcag cttcggactg aagccctgtc cgaagaagat gttatcgccc agcaccagac
    12121 aagaaggttc accgttgagg aaggtttcac cgatgataaa ggcctgcgcc agaccgtccg
    12181 gactgggctg ctcggcatat tccagctgga taccgaactc actaccatcg cccagcaggc
    12241 gctggaaata acctttatct tccggcgtag tgatgatgag aatttcgcgg ataccggcca
    12301 gcatcagcac cgacagcgga tagtaaatca ttggcttatc gtaaatcggc aacagttgct
    12361 tcgatacgcc gcgcgtaatc ggatgcaatc gggtgccgga accgcccgcc aggataatac
    12421 ctttcatgtt tacctccgct gaaaattagc ctttcagacc taaacgctcg ccctgatagc
    12481 tgccgtcctg cacctgctta caccagcttt cattagccag ataccactgc accgttttac
    12541 gcattccact ttcaaaggtt tcctgcggca gccagccaag ttcacgggca attttcgaag
    12601 catcaatggc ataacgcaga tcatgccccg gacggtcagc gacaaaggtg atcaggtcac
    12661 gatagtgcgc cacgccgtgc ggcttattcg gagccagttc ttccagcagc tcgcaaatgg
    12721 tttccacaac atcgagattc ttacgctcgt tgtgaccacc aatattatag gtttcaccga
    12781 ctttccctgt ggtcgccacg caatacagcg cgcgggcgtg atcatcaaca tacagccagt
    12841 cacggatttg ctgcccgttg ccatataccg gcagcggttt acccgccagc gcgttgagga
    12901 tcatcagcgg gatcagtttt tccggaaagt ggtaagggcc gtagttattc gagcagttgg
    12961 tgataagcgt cggcagacca taggtccgca gccaggcgcg caccagatgg tcgctgctgg
    13021 cttttgacgc ggaataaggg ctgctcggcg catacggcgt ggtttcggtg aagaaatcat
    13081 ccgtcgagtg caggtcacca tatacttcgt cggtagagat atgatgaaaa cggaacgctg
    13141 attttttatc ttccgtcagc gcattccagt aagcccgcgc cgcttcaagc aatgtataag
    13201 tcccgacaat gttggtttca acaaacgctg ccgggccgtc aatagaacgg tcaacatggc
    13261 tttcggctgc cagatgcatg acacagtctg gctgatgctc agtgaatacg cgcgccagtt
    13321 ctgcccgatc gcagatatca actttctcaa aggcaaagcg ctcgctttgc gcgaccggtg
    13381 ccagcgacat cagatttccg gcgtaggtca gcttatcgac cactaccacc gcgtcgctcg
    13441 tttcgttgat gatataacgc accagcgccg agccaataaa cccggcacca cctgttatca
    13501 gaatttttct catcagcgcc agactccttt ggcatcgacg acatactgct gatggacatt
    13561 gtcgccattg ataactttga actgactatg atcgaccagc atcaccagca cgtctgccgt
    13621 tgccagcgcc tcgtcaagct gcgccagagt acaaagcccg gtcagttttt tcggcaactg
    13681 gtggatgtta ggctcaacaa ccagagtttc gccgctatgc cactgggcga tcagttcagc
    13741 gatttccatc gccgggcttt cgcgcaggtc atcaatattc ggtttaaacg ccagaccaaa
    13801 gcaggcgatt ttcagttcac tggcgcgttt atcggtagca gcaaggcagt cggcaaccgc
    13861 cgctttcacc tgatcgataa cccagaacgg tttgtgatcg ttcacttcgc gcgcggtacg
    13921 gataagccgc gcctgctgcg ggttctgtgc cacgataaac cacggatcaa cagcaatgca
    13981 gtgaccgccc acgccaggac caggctgaag aatattaacg cgagggtgac gattcgccag
    14041 gcgaatcagt tcccagacgt taattccctg atcggcacaa atcagcgaca gttcgttagc
    14101 aaaagcgata ttcacatcgc ggaagctgtt ttcggtgagc ttacacattt ccgccgtccg
    14161 cgagttagtg acgacacact caccttcgag gaaaattttg tacagttcgc tggcgcgggc
    14221 cgaacaaacc ggcgtcatac caccaatcac gcgatcgttt ttaatcagct cgaccataac
    14281 ctggcccggt aacacgcgtt ccgggcagta agcaatgttg acgtccgcca actcacccac
    14341 ctgctgtggg aaagtgagat ctggacgcat ctctgctaac cattctgcca tcttctcggt
    14401 tgaccccacc ggcgaggtgg attcaaggat caccagcgcg ccttttttca gcactggcgc
    14461 aatggagcga gcagccgatt caacgtaggt catatctggc tcatgatcgc ctttaaacgg
    14521 cgtgggtaca gcaatcagcc aggcatccgc ttcaactggc gtcgtgctcg ctcgtaaaaa
    14581 accgccttct acggcagttt ttactacgct cgccaaatca ggttcgacga tatggatttc
    14641 gccacgattg atggtatcaa ctgcgtgttg gttgatatcg acaccaatca cctgtttttg
    14701 ccgagaggca aacgcagctg ctgtaggcag cccgatgtaa cccagtccga taacagaaat
    14761 ggtcgcaaaa ctcatagtga tatccgatta ttttttaacg cttccagaat gcgagagcat
    14821 gcctgaccat caccatacgg gttatgggcg cggctcatag cttgatattc gttttcgtct
    14881 tttaaaagac gcgtcacttc ctcgacaatt cgctgcttat ccgtgcctac cagacgcacc
    14941 gtacccgccg tcaccgcttc cggacgctca gtggtatcgc gcattaccag caccggtttc
    15001 cccagcgaag gcgcttcttc ctgaatgccg cctgagtcgg tcaaaatcag ccaggcgtgg
    15061 ttcatcagcc agacaaacgg taaatactcc tggggatcga tcagaatgac atttttcacg
    15121 tgccccagaa tgcgattgac cggctctctg acgttcgggt tgagatgcac cggatagaca
    15181 atctggatgt cctggtgcgt ggtggcgatg tctgccagtg catggcagat ttcttcaaaa
    15241 ccacgaccaa agctctcacg cctgtgaccg gtcaccagaa tcatcttttt atcgggatcg
    15301 ataaacgggt aatttgccgc cagttctgaa cgcagcgtat cgctgctcat cacctggtca
    15361 cgcacccata acagtgcatc aataactgta ttaccggtaa tgaagattcg gctatccgca
    15421 acgttttcac gcagcaagtt ttgccgggaa gtttcggttg gagagaagtg atacatcgcc
    15481 agatgcccgg tcaatgtacg gttagcctct tccggccacg gcgaatagag atcgcccgtg
    15541 cgcagaccag cctcaacgtg accaacagga atacgctgat aaaacgccgc caggctggtt
    15601 gccagcgtcg tcgtagtatc gccgtgaacc agcacgacat ctggtttgaa ctcggcaaga
    15661 attggtttta gcccttccag aatccgacag gttatctctg taaggccctg tcctggctgc
    15721 attatgttga gatcgtagtc aggtacaatg gaaaagagtt tcagcacctg atcgagcatc
    15781 tcccgatgct gcgcagtgac gcaaacttta gcctcaaaaa aaggatcttt tgccaacgca
    15841 tgcaccaacg gtgccatctt gatggcttcc gggcgcgtac caaatacagt cagtactttc
    15901 acatcgattc tcttcgaata agcggcgagc gcctttgcgc tcaccgcagc agtgttgcta
    15961 tttcgagcaa cggcgggtta atgcgacacc agccccgatc agtcccccga caatgcccca
    16021 cataatcatc aggaaggcac gacgtgggct atcgcgtttt accggttctt ccggcgtacg
    16081 caaatagcga taggtctgaa aacgcggatc cagggttgga ccaacattca gggtgtttaa
    16141 catggcccga ttctgatcat agtcgagatc aaaggccgga ccgacggcct gtaaattttc
    16201 cagtcgagcc tggagcattg gacgcccaag caggaacatt tctgaatcag gtaattcctc
    16261 ggcaggcaca tctgtcgcac tgcgcgaaat attatgctgc tcagcaattt tcagcgcctg
    16321 ttcaatactg ttcatccggc gatcgtagat ggctttcgcc acctcttcct gacgcttcac
    16381 ctgagctttc atctggatgg tgcgtgccgc ccatgcgcct ttcagctcat cattcagatg
    16441 gctggctgca cgctggctgg caaaagcaac atactgacgt aacaggttat tagcgtcagg
    16501 cgcagtttcg gcaatcagct tcacgctgtc attgaccgcg cgggtgaagt caccgggaat
    16561 aaacacgatg ttgttgatca tttcatccag caacgccgca tcggctttgc tattgcccac
    16621 catccgctgt ttgtaatagt cggtttgcag ccagaactct ctgcgggtat cccacgaggc
    16681 cagttgcata acaaactctt tataggcttc gtccatgacc gatggttggt cggcagaagc
    16741 catgtttgaa cggacatcca ggttacgcaa aaattgctgc tgtgagtaat atccccccag
    16801 catattcacc gttggacgat cggtaatcgc tgtcgagctc cactcctggc gagcaaaaaa
    16861 agtatacgcc agcgcgatta acgcaaacgc cagccccatg ccaataatcc acagcttccc
    16921 agcccacaag gtacgaaaca acccacgaat atccagttca ttttcagcgt cttcggccgg
    16981 tttcccaggc attggttgtg tcatcacatc ctcatttatt tggttaaatt ggggctgcca
    17041 ccacgatttc tacgcagtct gcgttttacg cgcttaataa agcgagcaac tttccaggca
    17101 cgcttaatgc aatatccata gaggaagaat gctagcaaaa agagcaccag catgacccac
    17161 tccgggacaa aatgagaata ttctgccagc acgccaatgg aagcgagcag tgctgcggca
    17221 agggtaatca gcacaaacgc ctggcgggaa gtaaacccgg cacgcatgat caaatggtga
    17281 atatgctggc gatcaggaga gaacggactc atgcctttac gcaggcgacg gtacataatc
    17341 gccaccatat ccattagcgg aatggcgatt atccacaaag cggtaaccgg gctgatggga
    17401 tgggttttgc cctgggtcgt ttcgagcagg atccagataa cggtaaaacc aatcagcgta
    17461 ctgcccgcat cacccataaa gactttgtag cggcgaccca ggataccaag gttaagcatg
    17521 atgtatggca ggatggcggc gatcatcgca aaacaccaga ttgcaaggct ggtttgcccg
    17581 tcgaaccaca aaatcatacc gattgctgca aacgagacgc aggacaaccc gcccagcaag
    17641 ccatcaatgc catcaaccat gttgaacgca ttaatggccg cccagacggc aaatagcgtc
    17701 aggaagtagc caaacggtcc gagcaccatc tcccaggagc caaagatata acccaggcta
    17761 ctgagataaa gtttgccgaa caccatcata acaatgccaa cagcggcctg tatggtggca
    17821 cggattttta cgctgatatc aaaacggtca tccagcgcgc caatgaaaac aagcacaccg
    17881 gcacaagcga gatagagaga tgcatgcgga atatagtaat cgacaattcc gaacgtgaag
    17941 caaatccctg cgtaaaccga aatcccccca acgagaggta tcaatccctg gtgacgtttg
    18001 cggaagttag gtttatccac taaaccgact ttttttgcca ccttacgggc aaaaaacaga
    18061 aacagtgtcg tgaataaaaa aatactgatg agatcagtac tcactgtcag taaattcaca
    18121 atgcatgctc tcagagaaaa ttattagcag aagtataacc acgaagacct ttattcagaa
    18181 gggaaagtcc gttccgaacc caccagtcct gtgcaaaaat taaccatttg atatgcaaat
    18241 tgcataacac accagcaaaa tccaggaatt ttcctaacat tgccagcgcg gcattaagat
    18301 tacagatata aaagcaaaac gccacgtaaa cacgtggcgt ttttggcata agacaaattt
    18361 atgagcgttt catcatttcg aagaaatcgt cattggtctt ggtcattgcc agtttattaa
    18421 tgaggaattc cattgcatcg atttcgccca tcgggtgaat gattttgcgc aggatccaca
    18481 ttttctgcag ttcttcctga gtagtgagca gctcttcttt acgggtaccg gaacggttgt
    18541 agtcgattgc cgggaagacg cgtttttcag cgatcttacg agagaggtgc agttccatgt
    18601 tgcctgtacc tttaaactct tcgtagataa cttcgtccat cttggaaccg gtatcgataa
    18661 gcgcggtagc gatgatggtc aggctgccgc cctcttccac gttacgcgcc gcaccgaaga
    18721 agcgtttcgg acgatgcagg gcgttggcat ccacaccacc ggtcagcact ttacctgacg
    18781 ccggaacaac ggtgttgtaa gcgcgcgcca gacgagtgat ggagtcgagc aggatgataa
    18841 cgtctttctt gtgctcaaca aggcgtttcg ccttctcgat caccatttcc gcgacctgaa
    18901 cgtggcgaga tgctggttcg tcaaaggtag aagcaacaac ttcacctttt accagacgct
    18961 gcatctcggt tacttcttcc ggacgttcgt cgatcagcag aaccatcagc acacaatccg
    19021 ggtggttgta agcaatgctc tgagcaatgt tctgcagcag catggtttta ccggctttcg
    19081 gcggtgccac aatcagacca cgctgaccac gaccgatagg tgatgccaga tccagtacgc
    19141 gagcggttaa atcttcagta gaaccgttac cacgttccat acgcagacga gagtttgcgt
    19201 gcagcggggt taagttctca aagaggattt tgttgcgggc gttttcaggt ttgtcgaagt
    19261 taacttcgtt aactttcagc agcgcaaaat agcgttcacc ttctttcggc gggcgaatct
    19321 taccagagat ggtatcacca gtgcggaggt tgaaacggcg gatttggcta ggggaaacgt
    19381 agatgtcatc aggaccggcg aggtaggagc tgtctgcgga acggaggaaa ccaaatccat
    19441 cctgcaatat ctccagtaca ccatcaccaa agatatcttc gccactcttt gcgtgctgct
    19501 tcaggatggc aaaaataatg tcctgcttac gcatacgagc caggttttcc agccccatat
    19561 tttcgccgag agtgatcagc tcagaaaccg gcgtattctt taattcggta agattcatag
    19621 tggtgtgagt tcttaaactt ggggtaaatc tcgaacttaa tgttgtgaat ggtatggcag
    19681 gatcatccat gcctgtttac ggtcatcgac tcatgtcttt tcgctgcctg gtcacaggag
    19741 agtacgcaga actgaaacga caagacggat tgagtgacga gccagaaatc tgttcacttc
    19801 gcatttaaga taaaacggga agcgttggaa attacaagat tcaaacttaa taaggtatgt
    19861 ttaatacgaa gtcaacacta agttagcatg actcacgccg ggcgtccagt ttttagcgac
    19921 ggggcacccg aacatgaaat tcccttacgc caggttagcg tcgaggaact ctttcaactg
    19981 acctttagac agtgcgccca ctttggttgc cgccacttca ccgtttttga acagcagcag
    20041 agtcgggata ccacggatgc catatttcgg cgcagtgcca gggttttgat cgatgttcag
    20101 ttttgcaacg gtcagtttgc cctgatattc gtcagcgatt tcatccagaa tcggggcgat
    20161 cattttgcac ggaccgcacc actctgccca gaaatcgacg aggatcgccc cgtccgcttt
    20221 gagtacatcc gtgtcaaaac tgtcgtcagt caggtgaata attttatcgc tcatatataa
    20281 ctccacagga ataagcctgg cgtgttggtt tcgttgttgg tgtaacatta accaactaaa
    20341 ggttgacttt atttcaccgg atacgctttc gtaaagcaat agtaagctga tattctacca
    20401 cactatgagc aaaacacatt taacagaaca gaagttttcc gacttcgccc tgcatccgaa
    20461 ggttgtagaa gcccttgaaa aaaaagggtt tcataactgt acgcccattc aggcactggc
    20521 ccttccgctg acgctggcgg gtcgtgacgt agccgggcag gcgcaaaccg gtaccgggaa
    20581 aacgatggcg tttcttacgt caacgtttca ttatcttctc tctcatcccg cgattgccga
    20641 tcgcaaggtg aatcaaccgc gtgccttaat tatggcaccg acgcgtgaac ttgccgtgca
    20701 gatccacgcc gacgcggagc cacttgcgca agctaccggc ctgaagctgg gtctggctta
    20761 cggtggtgat ggctacgaca aacagctgaa agtgctggaa agcggcgttg acattctgat
    20821 tggcaccacg gggcgtttaa ttgactacgc caagcagaac cacattaacc tcggtgccat
    20881 tcaggtagtg gtactggacg aagccgatcg tatgtacgat ctgggcttta ttaaagatat
    20941 ccgctggctg ttccgccgta tgccgcctgc aaaccagcgc ctcaacatgc tgttctccgc
    21001 cacgctttcg taccgggtac gtgaactggc gttcgagcag atgaacaatg ccgaatatat
    21061 tgaagtggaa ccggaacaga aaacgggcca ccgtattaaa gaagagcttt tctacccttc
    21121 taacgaagaa aaaatgcgtt tgctgcaaac gctgatcgaa gaagagtggc cagacagagc
    21181 gattattttc gccaacacca aacaccgttg tgaagagatc tggggccacc tggcggcaga
    21241 tggtcatcgt gtcggtttgt tgacaggcga tgtcgcgcag aaaaaacgtc tgcgtattct
    21301 tgatgaattt acccgtggtg atttggatat tctggttgcc accgatgttg ccgcgcgtgg
    21361 cctgcatatc ccggctgtga cgcacgtctt taactacgat ttacccgatg actgtgaaga
    21421 ttacgttcac cgtattggtc gtacaggtcg cgctggcgca agtggtcatt ccatcagcct
    21481 ggcatgtgaa gagtatgcat tgaatttgcc tgctattgag acctatattg gtcactcaat
    21541 tccggtaagc aaatacaatc cggacgcatt gatgaccgat ctgccaaaac cgctgcgcct
    21601 cacgcgcccg cgtacaggca atggtccgcg tcgtactggt gctccgcgta atcgtcgtcg
    21661 ttcaggttaa ataaaatgtc atactcaaaa tcattcaaac tgtatcaaga tggcaagaga
    21721 atgaatcccg atgcgcatgc tgtagtaagt gatttgggtg aaagaacgca gccaacacag
    21781 agacagattg aaggatgaag agtatgggtt ccacctcgtc gctgtatgca gccattgatc
    21841 tcggttcgaa tagttttcat atgctggttg tgcgcgaggt ggctggaagc atccagacgc
    21901 tgacgcgaat taaacgcaaa gtgcgtctgg ctgctggcct gaatagcgaa aatgccctgt
    21961 ctaatgaagc aatggagcgc ggttggcaat gtctgcgcct gtttgctgaa cgtctgcaag
    22021 atatccctcc ctcgcaaatt cgcgttgtcg ctacggcgac attacgacta gccgtcaatg
    22081 cgggtgattt tatcgccaaa gcacaggaaa tccttggctg cccggtacaa gtcatcagcg
    22141 gtgaagaaga agcccgcctg atttatcagg gggtggccca cacaacaggc ggtgccgatc
    22201 agcgcctggt ggtggatata ggcggtgcca gtactgaact ggtaaccggc acgggtgcac
    22261 aaaccacctc gttgttcagc ctgtcgatgg gctgcgtcac ctggctggaa cgctattttg
    22321 ccgatcgtaa tctggcgcag gaaaattttg atgctgcaga aaaagcggca cgcgaagtgt
    22381 tacgtccggt tgccgatgaa ttacggtatc acggctggaa agtgtgcgtt ggcgcttccg
    22441 gcaccgtgca ggcgttacag gaaatcatga tggcacaggg gatggatgaa cgcattaccc
    22501 tggaaaagtt gcagcaactg aaacaacgcg ccattcattg cggtcggctg gaagagctag
    22561 agattgacgg gctgacgctg gaacgtgcgt tagtgttccc gagtggtctg gcgatcctga
    22621 tagccatttt taccgaactg aatattcagt gtatgaccct ggcgggcggt gcgctgcgtg
    22681 aaggcctggt ctacggtatg ttgcatctta ccgtcgagca ggatattcgc agccgtacgc
    22741 tgcgtaatat tcagcgccgc tttatgatcg atattgatca ggcacagcgc gtagccaaag
    22801 ttgcggctaa cttctacgat caggtggaaa atgaatggca tcttgaagca ataagccgcg
    22861 atttgctcat cagcgcctgt cagcttcatg aaatcggcct gagcgttgac ttcaaacaag
    22921 cgccgcaaca cgctgcttat ctggtacgta atctggatct tcccggtttt acccccgcac
    22981 agaaaaaact gctggcgacg ctactgctca accagactaa tccggtcgat ctctcatcgc
    23041 tgcatcagca aaatgccgta ccaccgcgcg tcgcagaaca actctgccgt ttactccgcc
    23101 tggctatcat ttttgccagc cgtcgccgtg acgatctcgt gccagagatg acattacagg
    23161 ctaaccatga actgttgacc ttgacgcttc cgcaaggttg gctaacccaa catccgctgg
    23221 gtaaagagat tattgatcag gaaagccagt ggcagagcta tgtccactgg ccgctggaag
    23281 tgcattaatg agtaagtgcc ggatgcgatg ctgacacatc ttttccggcc ttgattattt
    23341 ccctcgtttt gccgccatca tcgctttcag attcgccaga tggctttgcc ctttctgcat
    23401 ccgttcttcg gcgctgacca ctttgcgctc ctgttcccaa atcaaatcat cctgcggcag
    23461 ctccagcagg aagcggctcg gctccgggcg caccagttct ccgtactgac ggcgttcttt
    23521 acacagcgta aaggtcaatt ccttctgggc gcgggtaatg ccgacatagg ccagccgccg
    23581 ctcctcatcg atattatctt catcgatgct gctctggtgc ggcaaaaacc cctcttccat
    23641 accgaccatg tagacataag gaaactccag ccctttcgac gcgtggagag tcatcagttg
    23701 cacctgatcc agctcttctt cactctcacc acgctccatc atgtcgcgca aagtaaagcg
    23761 cgtcaccacc tgggtgagcg tcatcggctc atccagttcg ctgccttcca gcatctccgt
    23821 catccagcta aacagttggt tgacgttctt catgcgcatt tcggcggctt tcgggctggg
    23881 cgatgtttcg tacagccagg attcataatc catgccgtgg atcagatcac gcaccgcggc
    23941 aatcggctcc cgctccgcca gacgctggat ttctgccaac cagtgagtga agcgggtcaa
    24001 tgcttcataa ccacgtccgc taagcgtctg actcaggccc atatcaaagc tggcggtaaa
    24061 catgctttta ttgcgcgtca tcgcccattc acccaacttt ttcagcgtcg ccgggccaat
    24121 ctctcgcttc ggcgtgttaa cgatacgcag gaacgcacta tcatcgtccg ggttagtcag
    24181 cacgcgcaga taagccagca agtccttgat ttcaggacga gagaaaaacg acgtaccacc
    24241 agaaattttg tacgggatgc ggttttgcat caggaatttt tcaaacaccc gcgactgatg
    24301 gttaccgcga taaagaatgg cgtaatcttt gtactgcgtt ttattgacga agtgatgggc
    24361 aatcagctcg ccagtaacgc gctcggcttc atgttcctcg ttattcgcgc ttaaaacttt
    24421 tagctctgtg ccgtaaccca gttcggagaa cagacgcttt tcaaagacgt gcgggttatt
    24481 ggcgatcagg atgttcgccg ctttcaaaat acgtccggaa gagcggtagt tctgctcaag
    24541 cttaatcacc ttcagcgccg gaaaatcctg actcagcagc accaggtttt gcggacgtgc
    24601 gccgcgccag gagtagatcg actggtcatc gtcacccacc acggtaaagc gcgcgcggct
    24661 gcccaccagc agtttcacca gctcatactg gctggtgttg gtgtcctgat attcgtccac
    24721 cagcaggtag cgaattttgt tctgccagcg ctcgcggact tcttcattgc gttgcaacag
    24781 caacgttggc aataaaatca gatcatcgaa atcgagaacg ttacaggctt tcaggtgtgc
    24841 atcatacagc ccataacaat gggcaaaaat acggtcccgc tcgccctttg cctctgccgc
    24901 cgcctgcgcc ggtgttttga gatcgttctt ccagttggag atggtcgaaa ttagctgttg
    24961 caggagaact ttgtcatctt caatcagccc ctcggtcaac tctttaagca aagcaagctg
    25021 atcggtatcg tcaaacagcg aaaagttagc cttcatcccc agcgccgcat actcgcgttt
    25081 gatgatatcc agccccagcg tgtggaaggt ggagatcatc agcccacgcg cctctttgcg
    25141 ccccagcgtc tgcccgacac gctctttcat ctcgcgtgcc gccttattgg taaaggtcac
    25201 cgccgcgata tgtcgcgcct gataaccgca accgcggatc agatgggcga ttttattggt
    25261 gataacacga gttttaccgg aacccgcgcc cgccagcacc aggcaggggc cggtaacgaa
    25321 ttcgacagct tgttgttggc cggggtttag acgcatagga aaatcgctca atgaaagtcg
    25381 ggaggggaaa ataacagcgt ggtagtatag ccagcctcat ccttaccact caaggcacga
    25441 tcatggcaaa aacagcagca gcactgcata tccttgtaaa agaagagaaa ctggctctgg
    25501 atcttctcga gcagatcaag aatggtgccg atttcggcaa gctggcgaag aaacactcca
    25561 tttgcccgtc aggcaaacgc ggcggtgatt tgggtgaatt ccgccagggt caaatggttc
    25621 cggcgttcga taaagtggtt ttctcttgtc cggtactgga gccaaccggc ccgctgcata
    25681 cccagttcgg ttaccacatc attaaggtgc tgtaccgtaa ctaacagcaa ggccttctcc
    25741 gggagaaggc cttgaactct gaatcgctat taacccgcaa cagcaatacg tttcatatcc
    25801 gtcatatagc cgcgcagttt cttacctacc tgctcaatcg catggctgcg aatcgcttca
    25861 tttacatcac gcagctgcgc gttatctacc gcaccttccg gaatagcttt acccaggtcg
    25921 cccggttgca gctctgccat aaacggtttc agcagcggca cacaagcgta agagaacaga
    25981 tagttaccgt actcggcagt atcggagata accacgttca tttcgtacag acgcttacgg
    26041 gcgatggtgt tggcaatcag cggcagttcg tgcagtgatt catagtaagc agattcttcg
    26101 atgatgccgg aatcaaccat ggtttcaaac gccaactcaa cgcctgcttt taccatcgcg
    26161 atcatcagta cgcctttatc gaagtactcc tgttcgccga ttttgccttc atactgcggc
    26221 gcggtttcga atgcggtttt gccagtctct tcacgccagg tcagcagttt cttatcgtcg
    26281 ttggcccagt cagccatcat gccggaggag aattcgccgg agatgatgtc gtccatatgt
    26341 ttctggaaca gcggcgccat gatctctttc agttgctcag agagcgcgta agcacgcagt
    26401 ttcgccgggt tagaaagacg gtccatcatc agggtgatgc cgccctgttt cagcgcttcg
    26461 gtgatggttt cccagccgaa ctgaatcagt ttttctgcgt atgccggatc ggtgccttct
    26521 tccaccagct tgtcgaagca cagcagagaa ccagcttgca acataccgca caggatggtt
    26581 tgctcgccca tcaggtcaga tttcacttcc gcaacgaaag aggattccag cacgcccgca
    26641 cggtgaccgc cggttgcagc cgcccatgct ttagcgatcg ccatgccttc gcctttcgga
    26701 tcgttttccg ggtgaacggc aatcagcgtc ggtacgccga atccacgttt gtactcttca
    26761 cgtacttcgg tgccagggca tttcggcgca accattacga cggtgatgtc tttacggatc
    26821 tgctcgccca cttcgacgat gttgaaaccg tgtgagtaac ccagcgccgc gccgtctttc
    26881 atcagtggct gtacggtgcg cactacatca gagtgctgct tgtccggcgt caggttaacc
    26941 accagatccg cctgcgggat caattcttcg taagtaccca ctttaaaacc attttcggtc
    27001 gctttacgcc aggatgcgcg cttctcggca atcgcttctt tacgcagagc gtaagaaata
    27061 tccagaccag aatcacgcat gttcaggccc tggttcagac cctgtgcgcc acagccgacg
    27121 atgactactt ttttaccctg aaggtagctc gcgccatcgg cgaattcatc gcgccccata
    27181 aagcgacatt tgcccagctg tgccagctgc tggcgcagat tcagtgtatt gaagtagtta
    27241 gccatggtga ttcctcgtga tgttgtgctt cttattgttc ggttcgcgtt tgcgagattg
    27301 aattcactat atgacaggaa atttattgcg gaaattgata tattcacaac gtcacattgc
    27361 aatttttgca acgtcaacat tgagggctgc gcctgtggat ttacgtgatc tgaaaacctt
    27421 cctgcatctg gcggaaagcc gccattttgg ccgtagcgcg cgggcaatgc acgttagccc
    27481 gtctacgctt tcgcgacaaa tacagcgcct ggaagaagat ctcggccagc cgctgtttgt
    27541 gcgcgataac cgcacggtaa cgctgacaga ggcgggcgaa gagctgcgtg ttttcgccca
    27601 gcaaacgctg ttgcagtatc agcaactgcg ccacaccatc gatcagcaag ggccgtcgct
    27661 ctctggcgaa ttacatatct tctgctcggt gaccgctgcc tacagccatc ttccaccgat
    27721 tctcgaccgc ttccgcgcgg aacatccgtc agtggagatc aaactctcta ccggtgatgc
    27781 ggccgatgcg atggaaaagg tggtcactgg cgaagcggat ctggcgattg cgggtaaacc
    27841 ggaaaccttg ccgggcgcag tggcgttttc gatgctggag aatctggcgg tggtgctgat
    27901 tgcccccgcg ctgccctgcc cggtgcgtaa tcaggtatct gtcgaaaagc ccgactggtc
    27961 tacagtgccg tttatcatgg ccgatcaggg accggtacgc cgccgcattg aactgtggtt
    28021 ccgacgcaac aaaatcagca acccgatgat ttacgccacg gttggcgggc atgaagcgat
    28081 agtatcgatg gttgcgctcg gctgtggcgt ggcgctgttg ccggaagtgg tgctggaaaa
    28141 cagcccggaa cctgtacgca atcgtgtaat gattctcgaa cgaagcgatg agaaaacgcc
    28201 gtttgagctg ggcgtgtgtg cgcaaaaaaa gcggctgcat gagccgctga ttgaggcgtt
    28261 ctggaggatt ttgccgaacc acaaatgacg ttgtcgcgcg ggtaggcctg ataagcgaag
    28321 cgctatcagg catttttccc taacccgcca aaaagaacct gaacgccggg ttattggttt
    28381 cgtcgtggca atcgtagccc agttcattca gccggatttc aaaatccggt tcatgatcgc
    28441 caagctcgaa cgccgccagt acgcgcccgt agtcggtacc gtggctgcga taatggaaca
    28501 gcgagatgtt ccagtgcgta cccagcgtgt tgaggaagcg cagcagcgcg cccggtgatt
    28561 ccgggaattc gaagctgtag aggcgttcct gcaacggatg cgatggacgc ccgccaacca
    28621 tatagcgcac atgcagcttc gccatttcgt cgtcggagag atcaaccacg ctgtagccgc
    28681 cgtcgttgag catctgcaaa atttctttgc gctcttcgag accgcggctc aggcgcacgc
    28741 cgacaaaaat gcaggcgttt ttggcatcag caaagcggta gttgaactcg gtgaccgagc
    28801 gcccgccaag caactggcag aatttgagga agctgccctt ctcttccggg atggtcaccg
    28861 ccagtaacgc ttcgcgctgt tcacccagtt cacagcgttc cgagacgtag cgcaggccgt
    28921 ggaagttcac gttagcaccg gaaagaatat gcgccagccg ttcaccgcga atgttgtgct
    28981 gggcgatgta ttttttcatc cctgccagcg ccagcgcgcc agagggttca gccaccgcgc
    29041 gtacatcttc gaacaggtct ttcatcgccg cacagatggc atcgctatcg acggtgatga
    29101 tgtcgtcaag atactcctgg cacaaacgga aggtttcatc gccgatgcgt tttaccgcga
    29161 cgccttcagc aaacagcccc acgcgcggca gatcaaccgg atgacccgca tccagcgctg
    29221 ctttcaggca ggcggagtct tccgcttcta cggcgatcac tttgatttgc ggcatcagct
    29281 gtttgatcag caccgccacg cccgcagcca gaccaccgcc gccgactggc acaaatacgc
    29341 ggtcgagatg ggcgtcctgc tggagcagtt ccagcgccag cgtaccttgc ccggcgatca
    29401 ccatcggatg atcgaacggc ggcacccagg tgaacccctg ctgctgtgac agttcgatcg
    29461 ctttggcttt cgcttcatca aagttcgcgc catgaagcag cacttcgccg ccgaatcctc
    29521 gtaccgcatc aactttgata tcggcggtgg cggttggcat gacgatcagc gccttcacgc
    29581 ctaaccgtgc ggaagaaaac gcgacgccct gcgcgtggtt acccgcagaa gcggtgatca
    29641 cgccgtgcgc tttttgttct tccgtcaggc ccgccatcat ggcgtatgcg ccgcgcagct
    29701 taaagctgtg tactggctgg cgatcttcgc gcttcaccag aatcacgtta tcaagacgcg
    29761 acgacagttt ttccattttc tgtagcggcg tgacctgcgc cgcttcgtag accggcgcgc
    29821 gcagcaccgc tcttaaatat tcggcacctt caggggcacc ggacaggggt tgtgagtctg
    29881 ccatcattaa ccccccagtt tcgatttatc gcgcaccgcg cctttgtcgg cgctggttgc
    29941 caggctgccg taggcacgca gcgcaaagga aacctggcgt tcacggtttt tcggcgtcca
    30001 ggctttgtcg ccacgagctt cctgcgcttc acgacgcgcc gccagttcag catcgcttac
    30061 ctgtaactga atgccacggt tcggaatgtc gatggcaatc atgtcgccgt cttcaatcag
    30121 accaatgctg ccgccgcttg ccgcttccgg cgagacgtgg ccgatggaaa gaccagaggt
    30181 gccgccggag aagcgtccgt cggtgatcag cgcgcaggct ttaccgagcc ccattgattt
    30241 caggaagctg gttgggtaga gcatttcctg catgccgggg ccgcctttcg ggccttcgta
    30301 acggatcacc accacatcgc cagcgacaac tttgccgccg agaatcgctt ctaccgcgtc
    30361 atcctggctt tcgtacactt tcgccgggcc ggtgaatttg aggatgctgt catcgacgcc
    30421 cgcggtttta acgatgcagc cgttttctgc gaaattaccg tacagcaccg ccagaccacc
    30481 gtctttgctg taggcgtgtt ccagcgagcg gatacagccg tttgcgcgat cgtcatcgag
    30541 agaatcccaa cggcaatcct gcgagaatgc ctgtgtagta cgaatgcctg ccgggcctgc
    30601 gcggaacata ttttttaccg cgtcatcctg ggtcagcata acgtcgtatt gttccagcgt
    30661 ttgcggcaac gtcaggccaa gtacgttttt cacatcacgg ttcagtaacc ccgcgcgatc
    30721 cagttcgccg agaataccga taacaccacc agcacggtga acatcttcca tatggtattt
    30781 ctgggtgctc ggcgcaactt tacacagctg cggaaccttg cgggaaagct tatcgatatc
    30841 actcatggtg aagtcgattt ccgcttcctg cgccgccgcc agcaggtgaa gtacggtgtt
    30901 ggtagagccg cccatcgcga tatccagcgt catggcgttt tcaaacgccg ccttactggc
    30961 gatattacgc ggcagtgcac tatcgtcgtt ttgctcgtaa taacgtttgg tcaattcaac
    31021 aatgcgttta ccggcattga ggaacagctg cttacggtcg gcgtgggttg ccagcaacga
    31081 gccgttgccc ggctgcgaca ggcccagcgc ttcggtcagg cagttcattg agttagcggt
    31141 aaacatcccg gagcaggaac cgcaggttgg gcatgcggaa cgttcaacct gatcgctctg
    31201 ggagtcagaa actttcgggt ctgcgccctg gatcatcgca tcaaccagat cgagcttgat
    31261 gatctgatcg gacagtttgg ttttcccggc ctccatcggg ccgccggaaa caaagatcac
    31321 cggaatattc aggcgcaggg aagccatcag catccccggg gtgattttgt cgcagttaga
    31381 gatgcagacc atggcgtcgg cgcagtgggc gttgaccata tactcaacgg aatcagcgat
    31441 cagttcgcga gatggcagtg aataaagcat ccccccgtgg cccatggcaa tcccatcatc
    31501 caccgcaatg gtgttgaact ctttggcaac gccgccagcc gcttcaattt gttcggcgac
    31561 cagtttacca agatcgcgca gatggacgtg acccggtaca aattgggtga acgagttcac
    31621 aaccgcgata atcggcttac cgaaatcggc gtcggtcatt ccggtggcgc gccacagcgc
    31681 acgagcaccc gccatattac gaccatgagt ggtggtggcg gaacggtact taggcatact
    31741 ttatttactc ccagtgtctg tctcgtaaat gggacggtgc gtgccgtccc attttttgta
    31801 tttattgatt aacttgatct aaccagcccc atttatcttc ggtttcgcca gtgaagaggc
    31861 cgaagaaggc ttgctgaatg cgtttggtaa ccgggccaca acggccttcg ccaacctgaa
    31921 taccgtctac gctgcgcact ggcgtgattt ctgccgccgt accggacata aacacttcat
    31981 ccgccaggta cagggattcg cgcgacagca cctgctcacg cacttcaatt cccagctctt
    32041 ttgccagttt gatgatggca tcacgggtaa tacccggcag cgcggaggag gtgaacggtg
    32101 gggtgaacag cacaccatct ttcacttcaa acaggttttc gcctgcgcct tcagagatat
    32161 aaccgttcac atccagcgcg ataccttcct gataaccgtg gcggcgcgct tcgctaccca
    32221 ccagcaggga agatagatag ttaccaccgg cttttgccgc cgtcgggatg gtgtttggtg
    32281 ctgcgcggtt ccaggaggaa accatcgcat cgatcccctg ctccagcgct tctgcaccca
    32341 gataggcccc ccacgggaaa gcggcaataa tcacatcggt tgagtatccc gctggcgggt
    32401 ttacgcccat gccaacatca ccgacgaaga tcagcggacg gatataggcg ctggtgagat
    32461 tgtttttgcg gatcacgtca cgacaagctt ccattagctc atcgatgctc tgcgaaaccg
    32521 ggaagcgata gattttggcg gagtcatgca gacgctgcat atgctcacga tggcggaata
    32581 caaccggtcc tttgtgcgag tcgtagcaac ggatgccttc aaaaaccgag gtgccatagt
    32641 gcagcgcgtg cgacatcaca tgcaccttcg cgtcttccca gcgaaccatc tccccattga
    32701 accaaatgta atcagctttc ttcgtggtca tttttatatt ccttttgcgc tcaggcgcgg
    32761 atttgttgtg atgtggttgt gctctggcag atggcaacgt gtgcgacgtc caccagttta
    32821 tttaactgac taaacagtaa gtcgaccgac cgtgggctgg caacggtcaa ttcgatattt
    32881 atattttgtg catcgctggc ggcggccata ttcattgaac agacgtggaa accacgatga
    32941 cgcaccacgc gtaaaacacg ttctaaggtt tccggattga agcgagccga tacattgacc
    33001 tgatgttgca tcatgataat ttctccaaca tttctgaatt actggcacca ggcggcacca
    33061 gcggccagac gttctcaaat tcgtcgattg agacatgaag caggtatggc ccatcactgt
    33121 tcagcatggt gtcgagtgcc gcttcaacct ggtctttatg ggtgatgtgt tggccaggga
    33181 tgccgaaggc gctggctaac atgaggaaat cggggttatc ggtaagggtg gtttcgctgt
    33241 atcgttcctg gaaaaacagt tgctgccatt gtcgaaccat ccctaaccgt tggttatcga
    33301 gtaagacgat tttcaacggt aactgcttgc gttttacggt gcccagctct tgcacattca
    33361 tcatgaaaga gccgtcaccg gagatacaga cgacagtatc gttcggtcgc gcgacttgtg
    33421 cgccaaccgc cgccggtaaa ccgaaaccca tggtgcctaa gccgctggag gtgatgaagt
    33481 tttccgggcg agtgtggacg atgtgctggg cagcccacat ctggtgctgc cccacatctg
    33541 tggtcacgat gcaatccgca gatttacgat ccgacagttg ttttaacaac aacggcgcgt
    33601 agatagcgtc accaggatgg tcgtaacgcc aggcatgttc atcacgcagt tgcgcgcagt
    33661 gttgctgcca gtcatcgata tttaacggct gctgtaatgc tggtaacaga gcatttaaat
    33721 catcctgtaa tgccacatgt gcctgacgca gcttgttcat ttctgccgga tcgatatcca
    33781 tatggataac gctggcgtgt ggtgcgaagg tgttcagttt gccggtcacc cggtcatcaa
    33841 aacgtgcgcc cacggcgatc agtagatcgc actcctgcac tgcgaagttc gccgctttgg
    33901 tgccgtgcat tcccagcatg cccagatagt acggataatc tgcttcaact gcgcccagcc
    33961 ctttcagcgt gcaggtggca ggcatttttg tggtagcgag aaattcacgc aaagccgaaa
    34021 ctgcctgcgc catgcccacg ccaccgccaa cgtacagcat cggtttttgc gcttttgcca
    34081 gcatctgacg cgcttgctcg acttcggcat gtgggaaagt cacttcgttt tcaacggtgg
    34141 tgaaccacgg ttccagatcg ccgctggcta actggatatc ttttggaata tcgaccaaaa
    34201 ccggaccagg acgaccagag ctggcgacgt caaacgcttc ggccatgata cgcggtaact
    34261 cttccagtga ttgcaccagg aaactgtgct tggtgcaggc taacgacaac cccagaatat
    34321 ccacctcctg aaatgcgtca gtgccgataa acggtgcgga tacctgaccg gtgatagcaa
    34381 caacggggat ggaatctaac agtgcgtccg caagcccggt tatcaggttg gttgcgcccg
    34441 gaccagacgt ggcgatacat acgccagttt tgccagtagc acgggcataa ccgatagccg
    34501 ccattgccgc accctgttca tgtcggcaca gcaagtgctc catgccgccg tcatacaatg
    34561 catcgtaaac cggcataatt gcgccacccg gataaccgaa aacggtattc acaccctgtg
    34621 cccgcaacgc atgtaccacc cactgtgcgc cattcatagt tagttccccg tcctgaatct
    34681 tgagaaacag aattttgtgc tgttattcat tgtctgctcc tcggttatgt ttttaaggtc
    34741 aaaaaaaacc cccggacctt tcggtgcggg ggtcttagtt cgttaaggct tgatctctaa
    34801 gcctttcctc gtccaagtgc agccccgcac ggtgggataa taatcaccac cacgctaatc
    34861 acgaccaggc taatcactcg tagaagggct gtcattttgt cttttcttgc atcttgttcg
    34921 aaggaatgcc taaagagtta ccatagattt tgcgaatagc acaagatatt ttttggccga
    34981 tttttatgat gtctttaata atttcaagag aaattgttgt tttttaaaaa gaaaatagtg
    35041 agtgaaaaaa attcaatttc agtatgaatg ttgttccctt cctcgtagtt catcctttgc
    35101 ccgctgcatt tttgcgagca tcattccacc gccagaacaa ctcaacttca cttgcacaaa
    35161 taaacaggaa tcagacacta aaattcacga attagcgggc tacagcagcg atatcttatg
    35221 cacataatgg tgactcaagg agggcttatg tcactgtcaa ttgttcatac ccgcgcagcc
    35281 ctgggagtaa atgcgccccc aatcactgtt gaggtacata tcagtaaagg tctaccaggc
    35341 ttaacgatgg tgggcttacc agaaacaacg gtaaaagaag ctcgcgatcg cgtgcgcagc
    35401 gccattatca atagcggata tgaatatccg gcgaaaaaaa tcaccatcaa ccttgctcca
    35461 gccgatctgc caaaagaagg gggacgatat gatttgccta tcgctattgc gttactggcg
    35521 gcctcagaac agcttacagc caataagtta gatgaatatg aattagtcgg agaactggcg
    35581 cttacaggcg ctctgcgtgg cgttcccggc gcaatctcca gtgcaactga agctattaag
    35641 tcgggcagaa aaattatcgt cgcgaaagat aacgaagatg aagtggggct aattaacggt
    35701 gaaggatgcc tgatagccga tcatctgcag gctgtctgcg cgtttctgga gggtaagcac
    35761 gctctcgaac gcccgaaacc aactaatgca gtatcccggg cgcaacaaca tgatctcagt
    35821 gatgttgtcg gtcaggagca aggaaagcga ggactggaaa ttaccgccgc tggcgggcac
    35881 aaccttttac tgattggacc gccgggaaca ggtaaaacaa tgctcgccag ccgtattaat
    35941 ggccttttgc cagatttaag caatgaagag gcactggaga gcgcagcgat attaagtctg
    36001 gtaaatgctg aatcagtaca aaaacaatgg cggcagcgcc cgttccgctc acctcatcac
    36061 agtgcgtcgt taactgcgat ggtgggcggt ggtgcaattc cagggccagg tgaaatttcg
    36121 ctggcgcata acggcgtgct ttttcttgat gagctacctg aatttgaacg gcgtacactg
    36181 gatgccttgc gagagccgat tgaatccggg cagatccatc tttcacgcac acgagcaaaa
    36241 ataacctatc cagcccgttt ccaacttgtt gcggcgatga atcccagccc taccggacat
    36301 tatcagggaa accataaccg ctgcacacca gagcaaacgc tgcgttatct caaccggctc
    36361 tctggtccct ttctcgaccg cttcgatctc tcactggaga tcccattacc gccacccggc
    36421 attttgagta aaacggtagt gccgggagaa agcagcgcca ccgttaaaca acgcgtaatg
    36481 gccgccagag agcgccaatt taagcggcag aataaactga atgcctggct ggatagtccg
    36541 gaaatacgcc aattctgcaa gcttgagagc gaagatgcgc agtggctgga agaaacgctg
    36601 atccatctgg ggttatcgat tcgtgcctgg cagcggttac tgaaagttgc acgaaccatt
    36661 gctgatattg atcagtccga cattatcaca cgtcagcatt tgcaggaggc agttagctat
    36721 cgtgccattg accgtttgct catccatctg caaaaactac tgacataaaa aaagggcatt
    36781 tcgccctttt tattaatcgt cagaatcggt gtagtcttca gcaccttcaa cctgcggttt
    36841 accgccggaa agggtgtgaa aacgttttgg acgcttgata cgcgtcatat acttggacca
    36901 cacgcgttct gcttctgtca ctggctcacg ttcgccacgg catactgcta cgaagagttt
    36961 ctcttcctcg gtaaccggct cgcgtttgcc aagatccaac tcattgaagg cataaccatg
    37021 acgctcaagc agttgtgcct ctttgatggt gaagtcacca tgacgagaga atccacgtgg
    37081 ataatgttta ttgtcgaaat atcgattagt cgtcgtaaag ctttccgcca tcctgcacgc
    37141 tcctaattct ttgaccgagc tagttatggc gcggagtatt agttacgctt gacagagtgt
    37201 aaaacaaaac atttaaatca taacgacaaa taattttgcg gagagcactg tggatacgga
    37261 attgttaaaa actttcctgg aagttagccg aacgcgtcac tttggtcgag cagctgaatc
    37321 gctctatctg acccaatcag ccgtgagctt tcgaatcaga caactggaaa atcaactggg
    37381 tgtgaacctt ttcacccgcc acagaaacaa tatccgttta accgctgctg gtgaaaaact
    37441 actgccttat gcagaaacgc tcatgagcac ctggcaggcc gcccgtaagg aggtggcgca
    37501 tacctcacga cataacgagt tttctatcgg tgccagcgcc tcgttgtggg aatgtatgct
    37561 taatcagtgg cttggacgct tgtatcaaaa tcaagatgcc catacaggct tacagttcga
    37621 agcgcgaatt gcccaacgac agtctctggt aaaacagctg catgaacgcc agcttgatct
    37681 tcttattacc actgaagcgc ccaaaatgga cgaatttagt agtcagttgc tggggtattt
    37741 cactttagcg ctttatacca gtgccccttc taaactaaag ggagatctta actatctgcg
    37801 actggagtgg gggccagatt ttcaacagca tgaggcaggt ttgatcggtg ctgacgaagt
    37861 tcccattctg acaaccagtt ctgctgaact ggcacagcaa cagattgcga tgcttaatgg
    37921 ttgcacctgg ctacccgtca gctgggcgcg taaaaaaggc ggcctgcaca ccgttgtcga
    37981 tagcacaaca ctttcacggc cgctttatgc catatggctg caaaatagcg ataaaaatgc
    38041 gttgattcgc gatctattga aaattaacgt gctggatgaa gtgtattaat ctgaatggct
    38101 ggcaaggatg ccggtagaag gatttacttc ggagaggtta tttcagataa aaaaaatcct
    38161 tagctttcgc taaggatgat ttctggcagg ggcggagaga ctcgaactcc caacacccgg
    38221 ttttggagac cggtgctcta ccaattgaac tacgccccta attagggtgg cggaacggac
    38281 gggactcgaa cccgcgaccc cctgcgtgac aggcaggtat tctaaccgac tgaactaccg
    38341 ctccaccgaa ttcttttaca accaccggtt ttatgaccgg ctt
//
LOCUS       DAPHCV010000038        37961 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.038, whole genome shotgun sequence.
ACCESSION   DAPHCV010000038 DAPHCV010000000
VERSION     DAPHCV010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37961
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.038"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(105..800)
                     /gene="aqpZ"
                     /locus_tag="RH033_001924"
     CDS             complement(105..800)
                     /gene="aqpZ"
                     /locus_tag="RH033_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HDV2991114.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAAAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
     gene            1225..2883
                     /locus_tag="RH033_001925"
     CDS             1225..2883
                     /locus_tag="RH033_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HDV2991115.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2880..3836)
                     /locus_tag="RH033_001926"
     CDS             complement(2880..3836)
                     /locus_tag="RH033_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HDV2991116.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYKGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3987..5102
                     /gene="macA"
                     /locus_tag="RH033_001927"
     CDS             3987..5102
                     /gene="macA"
                     /locus_tag="RH033_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HDV2991117.1"
                     /translation="MKKRKTVKKRYVIALVIIIVGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDNAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAVQ"
     gene            5099..7045
                     /gene="macB"
                     /locus_tag="RH033_001928"
     CDS             5099..7045
                     /gene="macB"
                     /locus_tag="RH033_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HDV2991118.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNATGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(7118..7342)
                     /gene="cspD"
                     /locus_tag="RH033_001929"
     CDS             complement(7118..7342)
                     /gene="cspD"
                     /locus_tag="RH033_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HDV2991119.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            7665..7985
                     /gene="clpS"
                     /locus_tag="RH033_001930"
     CDS             7665..7985
                     /gene="clpS"
                     /locus_tag="RH033_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HDV2991120.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            8016..10292
                     /gene="clpA"
                     /locus_tag="RH033_001931"
     CDS             8016..10292
                     /gene="clpA"
                     /locus_tag="RH033_001931"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HDV2991121.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(10636..10723)
                     /locus_tag="RH033_001932"
     tRNA            complement(10636..10723)
                     /locus_tag="RH033_001932"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10687..10689),aa:Ser,seq:gga)
     gene            complement(10977..11195)
                     /gene="infA"
                     /locus_tag="RH033_001933"
     CDS             complement(10977..11195)
                     /gene="infA"
                     /locus_tag="RH033_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDV2991122.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(11480..12184)
                     /gene="aat"
                     /locus_tag="RH033_001934"
     CDS             complement(11480..12184)
                     /gene="aat"
                     /locus_tag="RH033_001934"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HDV2991123.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(12226..13947)
                     /gene="cydC"
                     /locus_tag="RH033_001935"
     CDS             complement(12226..13947)
                     /gene="cydC"
                     /locus_tag="RH033_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HDV2991124.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFNYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNGSPIASLNEATLRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHNAPLVLLDEPTEGLDAT
                     TESQILELLAEMMHEKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13948..15714)
                     /gene="cydD"
                     /locus_tag="RH033_001936"
     CDS             complement(13948..15714)
                     /gene="cydD"
                     /locus_tag="RH033_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HDV2991125.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15837..16802)
                     /gene="trxB"
                     /locus_tag="RH033_001937"
     CDS             complement(15837..16802)
                     /gene="trxB"
                     /locus_tag="RH033_001937"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDV2991126.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLTGDSG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRNGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            17347..17841
                     /gene="lrp"
                     /locus_tag="RH033_001938"
     CDS             17347..17841
                     /gene="lrp"
                     /locus_tag="RH033_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HDV2991127.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17976..22043
                     /gene="ftsK"
                     /locus_tag="RH033_001939"
     CDS             17976..22043
                     /gene="ftsK"
                     /locus_tag="RH033_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDV2991128.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPAQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYSPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSAGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFTLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAQQPQYQQPQQPVTQQPQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            22202..22813
                     /gene="lolA"
                     /locus_tag="RH033_001940"
     CDS             22202..22813
                     /gene="lolA"
                     /locus_tag="RH033_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HDV2991129.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22824..24167
                     /gene="rarA"
                     /locus_tag="RH033_001941"
                     /gene_synonym="mgsA"
     CDS             22824..24167
                     /gene="rarA"
                     /locus_tag="RH033_001941"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HDV2991130.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            24258..25550
                     /gene="serS"
                     /locus_tag="RH033_001942"
     CDS             24258..25550
                     /gene="serS"
                     /locus_tag="RH033_001942"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDV2991131.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            25789..28233
                     /gene="dmsA"
                     /locus_tag="RH033_001943"
     CDS             25789..28233
                     /gene="dmsA"
                     /locus_tag="RH033_001943"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HDV2991132.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSALPAKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            28244..28861
                     /gene="dmsB"
                     /locus_tag="RH033_001944"
     CDS             28244..28861
                     /gene="dmsB"
                     /locus_tag="RH033_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HDV2991133.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28863..29726
                     /gene="dmsC"
                     /locus_tag="RH033_001945"
     CDS             28863..29726
                     /gene="dmsC"
                     /locus_tag="RH033_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HDV2991134.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(29761..30387)
                     /locus_tag="RH033_001946"
     CDS             complement(29761..30387)
                     /locus_tag="RH033_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDV2991135.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            30702..31850
                     /locus_tag="RH033_001947"
     CDS             30702..31850
                     /locus_tag="RH033_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2991136.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            32060..33490
                     /locus_tag="RH033_001948"
     CDS             32060..33490
                     /locus_tag="RH033_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV2991137.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(33491..33601)
                     /locus_tag="RH033_001949"
                     /pseudo
     CDS             complement(33491..33601)
                     /locus_tag="RH033_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(33700..34440)
                     /gene="pflA"
                     /locus_tag="RH033_001950"
     CDS             complement(33700..34440)
                     /gene="pflA"
                     /locus_tag="RH033_001950"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HDV2991138.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(34632..36914)
                     /gene="pflB"
                     /locus_tag="RH033_001951"
     CDS             complement(34632..36914)
                     /gene="pflB"
                     /locus_tag="RH033_001951"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDV2991139.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(36969..37826)
                     /gene="focA"
                     /locus_tag="RH033_001952"
     CDS             complement(36969..37826)
                     /gene="focA"
                     /locus_tag="RH033_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HDV2991140.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         9390 a         9264 c        10085 g         9222 t
ORIGIN      
        1 cccggccttc ggggaacgct tcagcgattt tgacgccacc aacacccgtg cggtttgtaa
       61 cgtcgaagat aaaacaacca gggcagataa aatgccaatg ctaattaatt acgcttttcc
      121 agcagggtcc ggtaaatcag accaccgata ataccgccga caattggcac cacccagaag
      181 aaccacagtt gttctaacgc ccagccgccc tggaagatag cagccgcggt gctgcgcgcc
      241 gggttaacag aagtgttagt caccggaata ctgattaagt gaatcagggt taaggccaga
      301 ccaatagcga tcggcgcaaa accagcagga gcgaatttgt cggttgcgcc gtggatcacc
      361 agcaggaaac ctgcactcaa taccagttca actaccagcg cggaaagcat ggaataaccg
      421 cctggcgaat gctcgccata accgttagaa gcaaaaccgc tggctgccgc gtcaaaacct
      481 gttttaccac tggcaattaa atacagcagc gccgccgcaa caataccgcc gacaacctgg
      541 gcaattacgt agccaacgac ttcttttgcc gggaaacgtc cgccagccca taaaccaata
      601 gtgaccgccg ggttaaaatg accaccagaa atatgaccaa cagcaaaggc catcgtcaga
      661 acggtcagac cgaatgccag cgccacgccg gcaaaaccaa tgcctaattc cgggaagcct
      721 gcggccagta cggcactacc acagccacca aaaacaagcc agaaagtacc aaaacattca
      781 gctgctaatt ttctgaacat atccacctca attaaaaatt gaccctgtga aaaatatggt
      841 cgttttatag ggtcgtcgta aaaaagtgac gacggaaata atgcgcggct attttaaaaa
      901 cgaaggcgag tcattcacca gataaataaa tccagtaaat ttgatttagg gcaacagcgg
      961 gttgccccat atagtcattt gtctgattga cagtgtagtg caagcaaaag atttaatcct
     1021 ttaggcgtaa taaaaataat ttatcatgct aattatttga ttttgttgtt tttgcaggct
     1081 tatcagcaag agggagtata acgcgattat tcgctcattt ttcagacatt tgccatgctt
     1141 aaatgtgatg tcatcacgta ttagcatggc ctttcccgtt atactgccag cgtaaaggat
     1201 aagtcacata tttctggagg ggatatgatt cttgagcgcg ttgaaattgt gggttttcgc
     1261 ggtatcaacc gtttgtcgtt gatgctggaa caaaacaacg tcctgattgg ggagaacgcg
     1321 tggggtaaat ccagcttgct ggacgcctta accctgctgc tatcgccaga atcagatctc
     1381 taccattttg agcgcgacga tttctggttc ccgccgggag atatcaacgg gcgagaacat
     1441 catctgcata ttattttgac cttccgcgaa tcgctgccag gccgacatcg ggttcgccgc
     1501 tatcggccgc tggaagcgtg ctggacgcca tgcaccgatg gttatcaccg tattttttat
     1561 cgtctggaag gggagagtgc ggaagacggc agcgtgatga cactgcgcag ttttctcgat
     1621 aaagacggac atccgattga tgttgaggat attaacgatc aggctcgtca tctggtgcgt
     1681 ttaatgccgg tgctgcgctt gcgtgatgcc cgttttatgc gccgtattcg taacggcacg
     1741 gtgccaaatg tccccaatgt ggaagtcacc gcgcgccagc tcgatttcct cgcccgtgaa
     1801 ttatcctcac atccgcaaaa tctctctgat gggcagattc gtcagggact ttccgcaatg
     1861 gtgcaacttc ttgagcatta tttttctgag cagggagctg gacaggcgcg atatcgttta
     1921 atgcggcggc gagccagcaa tgagcaacga agctggcgct atctggatat catcaaccgg
     1981 atgattgacc gacctggagg gcgctcgtat cgggttattt tgctcggcct gtttgctact
     2041 ttattgcagg caaaaggcac attgcgactg gataaagacg cccgtccatt gttgctgatc
     2101 gaagatccag aaacccgttt acaccccatt atgctttcag ttgcctggca tctgttgaat
     2161 cttctgccct tgcagcgcat tgccaccacc aactcgggtg agttactttc gttaacgccg
     2221 gtagagcatg tttgccgact ggtacgtgag tcctcgcgcg ttgccgcctg gcgtctgggg
     2281 ccgagtggct tgagtaccga agatagccga cgcatatcct ttcacattcg ttttaaccgt
     2341 ccgtcatcgc tgtttgctcg ctgctggctg ctggtggaag gggaaacgga aacctgggtt
     2401 atcaatgaac tggcgcgtca gtgcggacat cattttgatg ccgaaggaat caaggtcatt
     2461 gagtttgccc agtccgggct aaagccactg gttaaatttg cccgccgaat ggggattgaa
     2521 tggcatgtac tggtcgatgg cgatgaagca gggaagaaat atgccgctac ggtacgcagc
     2581 ctgttgaata acgatcggga agccgaacga gaacatttaa cggcgttacc ggcgctggat
     2641 atggaacatt ttatgtatcg ccagggattt tccgatgtgt tccaccgcgt ggcgcaaatc
     2701 ccggaaaatg taccgatgaa tctgcgcaaa attatctcga aagcgatcca tcgctcttcc
     2761 aaacccgatc ttgccattga agtggcaatg gaggccggac gtcgtggtgt ggactccgta
     2821 ccgacgctgc tgaaaaaaat gttctcacgc gtgctgtggc tggcgcgcgg tcgcgcggat
     2881 taaccgcgaa acatcgtggc catttgtggc tgaatagcgt cgagcatctc atagcgccga
     2941 cggtattcag cccgtttttt actggcgatt tcggcaatct cttttcgtgc tatctgtgcc
     3001 ggaaggcggt aatggcgttc agcatcacat acgccgccaa ccgactccca gaacgcgttg
     3061 taatcggcat ggatcttgcc ttctttatca cgataacgca ggctgcggta aatatgcgtt
     3121 tcattgctga cggcaataat ctgctctacc tgcaaacgtt gggcaaacag acaggccgct
     3181 tccatcacaa ggcgtttggg aaatagcccg tggcaggctt tcgtcgcatt ctggatttcc
     3241 tgatgtggaa tttcccattt tgcgccttgc agtccgccaa taaacattgt tcttttcccc
     3301 ttatattcac acagggtaaa cgtgatctct gccagaggaa taccttcgct gttgcggaac
     3361 aggattgtgc tgtcaccttc tttatccatt gagatcatca tggtcagctc aagcgtgaac
     3421 tgctcgccgt ttttgccttc cagtttagcc agttgcagcc cgggggtatt caaatataag
     3481 ctgaattctt ccgccgacat acacccgcgg agtaacgcat aatggtaacg taacgcctcc
     3541 agcaattgct tacggctaag attcgccgca aggtaagggc gatgcagacg cacaggcagt
     3601 cgcggctggc gcgttaacaa cacattgaga ttgggccagt gggaaagttc gttcatccac
     3661 tcaacgctta aacgcggcat aatcaacgag cgcagcaaaa atttctggcg aaaactacgg
     3721 cgatgccaga atttacccgg ccgacactgt ccacgtgcca gactaagaaa aagtgacagg
     3781 ctgctgagag attcagatgg cgtaaaggtc cgttcagtta gctgcgacat attcatgaaa
     3841 tcaatggtta tacatgacgt cgatttcacc attgcgtatc ttaaccaaac atcaatagtg
     3901 tgattactaa tgtaaatttt agggttttgt tgatatttcg ttgaagttaa tgacccggat
     3961 tggcatatgg agtattcaga atatttatga aaaagcggaa aaccgtgaag aagcgttacg
     4021 ttattgcgct ggtgataatc atcgtcgggc tgattacgtt atggagaatt cttaacgcac
     4081 ccgtgccgac ttatcagacg ctgattgtgc gccccggtga tttacagcaa agcgtgctgg
     4141 cgaccggaaa gctggacgcg ctgcgtaagg ttgatgtggg cgcgcaggtc agcggtcagt
     4201 tgaaaacgct gtcggtggcg attggcgata aagtaaaaaa agaccagctt ttaggggtta
     4261 ttgatcctga acaggctgaa aaccagatta aggaggtgga agcaacgctg atggagctac
     4321 gcgcgcagcg gcagcaggcg gaagcggagc tgaaactggc gcgggtgacg tattcccgtc
     4381 agcaacgtct tgcacaaacg caagcggttt cacagcagga tctcgataac gccgcgacgg
     4441 agatggctgt gaaacaggcg caaattggca ccattgacgc gcaaatcaag cgcaatcagg
     4501 cctctctcga tacggctaaa accaatctcg attacacccg catcgttgcc ccgatggccg
     4561 gggaagtcac gcaaatcacc actctgcaag gccagacggt gattgccgca caacaagcac
     4621 cgaacattct gacgctggca gatatgagca ccatgctggt aaaagcgcag gtttctgaag
     4681 cggatgtaat ccacctgaag ccggggcaaa aagcctggtt tacggtgctt ggcgatccgc
     4741 tgacgcgcta tgaggggcaa atcaaggatg tgctaccgac gccggaaaag gttaacgacg
     4801 ctattttcta ttacgcccgt tttgaagtcc ccaaccccaa tggtttgctg cggctggata
     4861 tgaccgcgca agtgcatatt cagctcaccg atgtgaaaaa tgtgctgacg atccctctgt
     4921 cggcattagg cgatccggtt ggcgataatc gttataaagt caaattgttg cgtaatggtg
     4981 aaacacgcga gcgtgaagtg acgattggcg cacgtaacga taccgatgtt gagattgtca
     5041 aagggctgga agcgggcgat gaagtggtga ttggtgaggc caaaccagga gctgtacaat
     5101 gacgcccttg ctcgaattaa aggatattcg tcgcagctat cctgccggtg atgagcaggt
     5161 tgaggtgctg aagggcatca gcctcgatat ttatgccggt gagatggtgg cgattgttgg
     5221 cgcttcgggt tccggtaaat cgaccctgat gaatattctc ggctgtctgg ataaggccac
     5281 cagcggcacc tatcgcgtcg ccggtcagga tgttgccacg ctggacgccg atgcgctggc
     5341 gcaactgcgc cgcgagcatt tcggctttat tttccagcgt taccatttgc tttcgcattt
     5401 aaccgccgag cagaacgttg aagtacccgc cgtctatgcc ggtcttgagc ggaaacagcg
     5461 gctgcttcgc gcccaggagt tactgcaacg actggggctg gaagaccgta cagagtatta
     5521 cccggcacag ctttcgggtg gccagcaaca gcgcgtcagc atcgcgcggg cattgatgaa
     5581 cggcggtcag gtaattcttg ccgatgaacc gaccggcgca ctggacagcc attctggcga
     5641 agaggtgatg gcgatcctgc atcagctgcg cgatcgcggg catacggtga ttatcgtcac
     5701 ccacgatccg caggttgctg ctcaggccga gcgggtgatc gaaattcgcg acggcgaaat
     5761 tgtgcgcaat cctcccgcca ttgaaaaagt gaatgctact ggcggaacgg aacctgttgt
     5821 caacacggcg tctggctggc ggcagtttgt cagcggtttt aacgaggcgc tgacgatggc
     5881 atggcgggcg ctggcagcga ataaaatgcg tactttattg accatgctgg ggattataat
     5941 cggtattgcg tcggtggttt ccattgtcgt ggtgggcgac gccgccaaac aaatggtact
     6001 ggcggatatt cgttctattg gtacgaatac tattgatgtc tatcccggga aagattttgg
     6061 cgatgacgat ccgcaatatc agcaggcgct gaagtacgat gacttaatcg ccatccaaaa
     6121 acaaccgtgg gtcgcttcag ccacacctgc cgtctcgcaa aacctgcgcc tgcgttataa
     6181 caatgttgat gttgctgcca gtgccaatgg cgtgagcggc gattatttta atgtctatgg
     6241 catgaccttc agtgaaggaa acacctttaa tcaggagcag ctgaacggtc gtgcgcaggt
     6301 ggtggtgctc gacagtaata ctcgccgcca gcttttcccc cataaagcag atgtggttgg
     6361 cgaggtgatt ctggtcggca atatgcccgc cagagtcatt ggtgtggcgg aagaaaaaca
     6421 gtcgatgttt ggtagcagta aagtgctgcg tgtctggcta ccttacagca cgatgtccgg
     6481 gcgagttatg ggccagtcgt ggctgaactc cattactgtc agggtgaaag aaggatttga
     6541 cagcgctgag gcggaacagc aactcacgcg tttactttca ttgcgccacg gtaagaagga
     6601 tttctttacc tggaacatgg acggcgtctt gaaaaccgtt gaaaagacca cacgtacttt
     6661 acaactgttt ctgacgctgg tggcggtgat ttcgctggtg gtgggcggta ttggtgtaat
     6721 gaatattatg ctggtgtcag tgaccgagcg gacgcgggaa attggcattc gcatggctgt
     6781 aggtgcgcga gcaagcgatg ttttgcaaca gttcctgatt gaagccgtac tggtttgcct
     6841 ggtcggtggc gcgttgggaa taacactgtc actgttaatt gctttcacct tacagctttt
     6901 cttacccggc tgggagattg gtttttcacc gttggcgctg ctgctggcgt ttctctgctc
     6961 gacggtcacc gggattttat ttggctggtt acccgcacga aatgcggcac gactggatcc
     7021 agtagatgct ctggcacgag agtaattttt gagataaaaa tgccagccga tcgggctggc
     7081 attttgcctt taggatgtac acaatgagac agaagagcta tgcgactgcc gcttctactt
     7141 cgacgggcac aataacactg gcgtgattgc cttttggccc ctggtggaca tcaaactgaa
     7201 cggattgtcc agcttttagc gttctgtaac catccatctg aatggtggaa taatgagcga
     7261 aaatatcttc gccgccgcct tcagggcaga tgaaaccaaa ccctttggca ttgttgaacc
     7321 acttaacagt acccttttcc atgcttcgac atccttcgca aatcttatac aagtaagatg
     7381 gaataaaccg gggtcagaga gggggctgtt caaaacctcg ccaactctag aaatacaatt
     7441 tagagaatta gggcgagccg tcaagcattt gacaggggcc agggggcagg tatgaatcaa
     7501 aaatttgaag cagttaacgc tattgacagg aatgtgacag atgtcgctga tgccaacgat
     7561 agatgatagt tatctatcat gtggagtaga ttggtcaggc aaataagctc ttgtcagcgg
     7621 cagggcgttc tgccgataac cgtaaccgaa gatgataact gacaatgggt aaaacgaacg
     7681 actggctgga ctttgatcaa ctggcggaag aaaaagttcg cgacgcgcta aaaccgccat
     7741 ctatgtataa agtgatatta gtcaatgatg attatactcc gatggagttt gttattgacg
     7801 tgttacaaaa attcttttct tatgatgtag aacgtgcaac gcaattgatg ctcgctgttc
     7861 actaccaggg gaaggccatt tgcggagtct ttaccgctga ggttgcagaa accaaagtgg
     7921 cgatggtgaa caagtacgcg agggagaatg agcatccatt gctgtgtacg ctagaaaaag
     7981 cctgaatgca ggcataaaaa ttgggggagg tgcctatgct caatcaagaa ctggaactca
     8041 gtttaaatat ggctttcgcc agagcgcgcg agcaccgtca tgagtttatg accgtcgagc
     8101 acttgttact ggcgctgctc agtaacccat ctgcccggga ggcgctggaa gcgtgctctg
     8161 tggatttggt tgcgctccgt caggaactgg aagcctttat tgaacaaacc acacccgttc
     8221 tgcctgccag tgaagaggag cgcgacacac agccgacgct gagttttcag cgtgtactgc
     8281 aacgtgcggt cttccatgtc cagtcctccg gtcgcaatga ggttaccggt gcaaacgttc
     8341 tggtcgctat ctttagcgaa caggagtcgc aggcggcata tctgttgcgc aaacatgaag
     8401 tcagccgtct cgatgtggtg aactttatct ctcatggcac gcgtaaagac gagccgacac
     8461 agtcttctga tcctggcagc cagccaaaca gcgaagaaca agctggtggg gaggaacgta
     8521 tggagaattt cacgacgaac ctgaatcagc ttgcacgtgt gggcggaatc gacccactga
     8581 ttggtcgtga gaaggagctg gagcgtgcta ttcaggttct ctgccgtcgc cgtaaaaaca
     8641 acccgctgct ggtgggggag tctggtgtcg gtaaaaccgc gattgcggag gggcttgcat
     8701 ggcgaattgt tcagggcgat gtgccggaag tgatggctga ctgtacgatt tactctctcg
     8761 atatcggttc tctgttagcg ggcacaaaat atcgcggcga ctttgaaaaa cgttttaaag
     8821 cgttgctcaa gcagctggag caggacacta acagcatcct gtttattgat gagatccaca
     8881 ccattatcgg tgcgggtgca gcgtctggtg gccaggtcga tgcggctaac ctgattaaac
     8941 cgttgctctc cagcggtaaa attcgcgtaa ttggttcgac aacctatcag gagttcagca
     9001 acattttcga gaaagaccgt gctctggcgc gtcgcttcca gaaaattgat attactgaac
     9061 cgtcgataga agaaaccgta cagatcatca atggcctgaa accgaagtat gaagcgcacc
     9121 acgacgtgcg ttataccgca aaagcggtgc gtgcagcggt agagctggcg gtgaaataca
     9181 ttaacgatcg tcatctgccg gataaagcca ttgacgttat cgacgaagcg ggcgctcgcg
     9241 cacgcctgat gccggtaagc aaacgcaaga aaaccgttaa tgtggcggat attgagtccg
     9301 tggtggcccg tattgcgcgc attccagaga agagtgtttc gcagagtgac cgcgataccc
     9361 tgaaaaacct cggcgatcgc ctgaaaatgc tggtcttcgg tcaggataaa gccattgagg
     9421 cgctgactga agccattaag atggcgcgtg caggtttagg tcacgaacat aaaccggtcg
     9481 gttcgttcct gtttgccggt cctaccgggg tcgggaaaac agaggtgacg gtacagcttt
     9541 cgaaagcgtt gggcattgag cttctgcgct ttgatatgtc cgagtatatg gaacgccata
     9601 ccgtcagccg tctgattggt gcgcctccgg gatacgttgg ttttgatcag ggcggtttgc
     9661 tgactgatgc ggtcatcaag catccacatg cggtgctgct gctggacgaa atcgagaaag
     9721 cgcacccgga cgtgttcaat attctgttgc aggtgatgga caacggtacg ctgaccgata
     9781 acaacggacg caaagcggac ttccgtaacg tggtattggt gatgaccacc aacgctgggg
     9841 tacgagaaac tgagcgtaaa tcgattggtc ttatccacca ggacaacagt accgatgcga
     9901 tggaagagat caagaagatc tttacgccgg agtttcgtaa ccgtctcgac aacattatct
     9961 ggttcgatca tctctccacc gacgtgatcc atcaggtagt ggataaattc atcgtcgagt
    10021 tgcaggttca gctggatcag aaaggtgttt ctctggaagt gagccaggaa gcgcgtaact
    10081 ggctggccga gaaaggttac gaccgggcaa tgggcgcacg tccgatggcg cgtgtcatcc
    10141 aggacaacct gaaaaaaccg ctcgccaacg aactgttgtt tggttcgctg gtggacggcg
    10201 gtcaggtgac ggttgcgctg gataaagaga aaaatgagct gacttatgga ttccagagtg
    10261 cacaaaagca caaggcggaa gcagcgcatt aatctaattg ttaggtaggt tggtgaagtc
    10321 cgtaatctcg aaagaggtta cggacttttt gtttatgggg tggaggaggt tctgaccctt
    10381 tttttgatga tgatggtaat ttgttgataa ttagtgctgc gggaaggtaa ggataaaaaa
    10441 gggtggtagc aggaaaatga gatggttttg ctttattaac aacgggctag acgtgtagta
    10501 tttgagttca ctgccgtaca ggcagcttag aaagccaacc gaaagaaaaa ctcgattttt
    10561 ataccgttca ctgccgtaca gacagataaa atgcgaaaaa aaaaccgtac tttcgtacga
    10621 gctcttcttt gaatatggcg gtgagggggg gattcgaacc cccgatacgt tgccgtatac
    10681 acactttcca ggcgtgctcc ttcagccact cggacacctc accaaattgt tttgctacca
    10741 aacctcatgg gtggcaacgg ggcgctacta tagggagttg gagtaaaacg gtcaagaaga
    10801 attttaatga taattattgt ttgctcatac tgtaaacaag ttgtgcagta tatctacatc
    10861 gagacaagtt atggacttat acttccaaag tacttcatac atatcacaaa ataaaaaggc
    10921 cggctatacc gaccttttac tcgttctttc tcttcgccca tcaggcggta aaacaatcag
    10981 cgactacgga agacaatgcg gcctttgctc aggtcgtacg gggtcagttc aacagtcact
    11041 ttgtcgcccg tcaggatgcg gatgtagttt ttgcgcattt taccggagat gtgtgcagta
    11101 accacgtgac cgttttctaa ctctacgcgg aacatggtat taggcaacgt ttcaagaacg
    11161 gtaccttgca tttcaatatt gtcttctttg gccatctaat cctctggggt atcactaccg
    11221 taatttgaac cggcaagata atgccgaagt tctgtaaata agtaaagatt tgcgcgctaa
    11281 atcgcaacaa acaggttcgg cacattactc cgaaaacaca cggctaagcc gcaccaaaag
    11341 cgcaacgtat aagggagcgg tgagataaac gatgggcgtt acctgacgcg aaaaattcct
    11401 tatcggcagc ggggtaatga gcgtaaccaa ctctgcgacc gcaattataa cactctgggg
    11461 agaaatgtgc cgaaaacatt cattcttgtg gtgaaaacaa gcaccgtggt acccagaaat
    11521 tattcggcaa tcgtccgagg cgcatttgat tgagataatt gaggtaatcc cggcggggaa
    11581 tttcgcaggc accaagcgac gctgtgtgat cgttaaggac ctggcagtcg ataagtttac
    11641 cgccatgacc aataaattcc tcacagaata ccagaagcgc cgttttagac gcattttcca
    11701 tccggctgaa catggactcg ccacaaaata gcgttccctg ggccacgccg tacataccgc
    11761 cgacaagctc atcttcacgc cagacttcaa tagagtgggc atgaccgagt tcgtgaaggc
    11821 gatggtaggc ttcgaccacg ccacgcgtga tccaggttcc ttcttcgcga tcgctggcac
    11881 agccttcaat gacctgacca aaagcgtaat tcatcgtgac tcgatagggc gagcgtttat
    11941 gaaatcgctt catactacgg ctgatatgca gtgattcagg ccaaagcacc gcgcggggat
    12001 cgggcgacca ccagaggatg gggtcacctg gagaaaacca cggaaaaata ccacgctggt
    12061 aagccattaa caggcgcgca gggctaagat cgcctcccag tgccagcagg ccgttaggct
    12121 cacgtaatgc gccttccggg gaagggaagg ctattgaatg gcgagaaagc tgaaccaggc
    12181 gcatgaccgc aaaactccac gcaagtcgga tcgttcaata atagcttaca aaccctgctt
    12241 aaactggtaa taacgcccct gtctggcaag cagttctgcg tgagtacctt gctcaataat
    12301 ttgcccgttg tccatcacta ttatttgttg gaaacgagag agtccgcgaa gtcgatgggt
    12361 gaccattaac accgttttct catgcatcat ttctgcaagc aattcaagga tctgactttc
    12421 ggttgtggca tctaagcctt ctgtaggttc atccagcaat accagcggcg cattatgtaa
    12481 cagcgcacgg gcgatagcca gacggcgtag ttcaccaccg gagagctggc gtccgccttc
    12541 acccaaccaa ctgttgagac ctgcatcctc gagcagcttc tccagaccaa cgcgacgtaa
    12601 gatctccgcc agagcctcat cactactgcc aggcgaggcg agtaacagat tatcacgcag
    12661 cgtggcgcta aacagatgca ctcgctgagg aacaacgctg atggtctgtc gtagagtcgc
    12721 ttcattcagg ctggctatgg ggctaccgtt aagcaaaatc tcgccctgtt gcgggtccca
    12781 tgcgcgggtc agcagttgta acagtgttga tttgccgcat ccggttcgcc cgagaatcgc
    12841 tatatgttcc ccggcgttta cctgaagaga aatcccttta agtgcctgtt gggattgctc
    12901 cggataattg aattgaacat cccgtaacgt cagcgaaacg cgatcggcaa cacgagtttg
    12961 ggtatcaggg aaggtgactt ccggtttttg atccgttaag tcagtgatcc gtacggcaga
    13021 ggcaatgact tgccccagat gctgaaatgc acccgttact ggtgccagtg cttcaaacgc
    13081 ggctaacgcg cagaagacaa acagggcaat taacgcgccg ggttgagcat tgccgccaac
    13141 gccgccagac gccatccaca gcatcaggat caccgctaac gcgccaatga gcagcattat
    13201 cgcttgcgac aatgcggtga gttcagattg acgacgttgc gcttccagcc actgaatttc
    13261 tgtattctct aattgcgtgc gataacgatc gctggcacca aagatggtca gctcagcttg
    13321 cccttgcagc caggccgtca gttgttggcg atactgcccg cgaagatgag tcagattttg
    13381 cccggtgctt tttcccgcac gataaaacag tggtggcata aggaaaagcg tcagtaacat
    13441 aatgccgccc agcgtaaagg cgagggtgaa atcaaggaaa cttaacccga ttgtcaccac
    13501 cataatcacc acaaaagcgc ccaccagcgg cgagataacg cgcaggtaaa gatgatcgag
    13561 cgtatcaaca tccgccacca cgcggttgag caattcgccc tgacgatagc gcgccagtcc
    13621 ggcaggggat aggggcagca atttgctgaa ggtgtaaatg cgcagatgct gcaacacgcg
    13681 gaaagtcgcg tcgtgactta ccagacgttc aaaatagcgc ccggcagtac gggtgattgc
    13741 tgcgccacgc acgcccgcag cgggtagcat atagttgaag ctgtacagtc cggcaacccc
    13801 cgctaccgct gaggcggaga ggaaccagcc ggaaagtgtc aacagaccga tactggcgag
    13861 cagcgtgaca attgccagca caataccaag acttaacatc catttatgac gtttatacag
    13921 tgccagatag ggtagcaaag cgcgcattta aatctcctcc tgacgatggg ccagcaatgt
    13981 ggcgaaagga ccaccagcca cgcttaattc cgcgtaacgt ccttgctcaa taatctgacc
    14041 atcctgcatt acccaaatga catcccagtc agcaagatct tctaactggt gggtgaccat
    14101 taacgttgtc tggcgcagag aggcggcatt aagcgcctcc attacgcgct gttcactgtg
    14161 agcatcaagg ctggcagcgg gttcatccaa cagtaatagc gaacagggat ttagtaatgc
    14221 tcgggccacc gccacgcgct gcgcctgccc cacggaaagg cgggcagcct ggtcgccaac
    14281 aggcgtatca acgccttgtg gcaggagcgg tagaaactcg ctgacccagg cgttatccag
    14341 cgctgcttgt aattcttgtt cgctggcatc aggtcgcgcc agtagtacgt tatcccgcaa
    14401 tgttgctgcc ggtaattgtg ggttttgccc aacccaggag agatgtttac gccatgattc
    14461 tggtgataaa tcacgtaatt ctatcccgtt gattcgtagc gatccctgat atgagagaaa
    14521 accagaaagc gcgttcagca gtgagctttt acctgaaccg ctgcgaccaa ccaacactgc
    14581 acgttggcct gctggcaaag taaagttcag cggtccagcc agcgttttac cttctggtga
    14641 tgtaataaac agatcctcgg cttcaatggt caccggatcg gttgatgcta attccgcctc
    14701 accacgttgc ggatgggcga gcggggtttc cataaacgtt ttcagactgt cagctgcgcc
    14761 aacagcctgg gctttagcat gataaaacgt accgagatcg cgtaatggct ggaaaaactc
    14821 cggcgcaagg atcagggcca gaaaacccgc agccagcgtc acgccagtat catagtgacc
    14881 aaaatccagc tcgccgagat aggaaaaacc aaagtagacc gccaccagag caattgacag
    14941 cgaggtaaaa aattcgagaa tgccggagga taaaaacgcc agccgtagca cttccattgt
    15001 ccgttggcgg aaatcttccg aagcagaacg aatactttca atttcagctt caccacgacc
    15061 aaaaatacgc aatgtttcca tgccgcgcag gcgatcgaga aaatgcccac ttaagcgagc
    15121 aagagcgaga aagttacgtc ggttagcatc ggcagccccc attccaacca gcgccataaa
    15181 taacggaatt agcggtgcag tgcccagcag aatgagcgcc gcagcccagt ttgaggggaa
    15241 gatagccacc acaatcagca acggcaccga cactgccagc gccatttgcg gcaggtagcg
    15301 tgcatagtaa tcatgcatat cgtcaatttg ctcgagtacc agcgtcgccc agctccccgc
    15361 cggtttaccc tgaatccacg ctggccctgc ttgttgcaga cggtcgagaa cctgacggcg
    15421 gatggcaaag cggatatgct gcccggcgtg ataacccacc cgttcgcgta accagaccac
    15481 ccatgcgcgc agtacaaagg tcagaaccag taacgtaaag ggaagcagca gggcttcacg
    15541 gggaatattc tccataatca tatgttgcag aattcgcgcc atgaaccagg cctgggcaat
    15601 gatcaatatg ccgctcacaa agcccagcag acgagaaata ttcagccaac gttgggagat
    15661 gacgctttgc tgttttaacc agcgggttaa ctctttttga cgggatttat tcattgcacg
    15721 cttagcaggt gagttatcag aattatttgc agagcaatgt tacaacgagg aaaaaataaa
    15781 ggcgacccat agtcgcatgg tgtcgccttc tttacttttg ttactgattt gtaaaattat
    15841 tttgcgtcag ctaaaccatc gaggtagcgt tccgcatcaa gtgctgccat gcagcctgta
    15901 ccagcagatg taatagcctg gcgataaatg tgatccatca cgtcgcctgc ggcaaagacg
    15961 ccagggatgc tggtctgggt ggcattacca tgaatacccg actgtacttt gatgtagccg
    16021 ttttccagtt ccagctgccc ttcgaaaatc gcagtattcg ggctgtgacc gatagcaaca
    16081 aacagaccgg caacgtcgag tgactcgatg ttatcgctgt tttgcgtatc gcgcagacga
    16141 acgccagtga cgcccatctg atcgccggtc acctcttcca gcgtacggtt ggtgtgtaga
    16201 atgatgttgc cgttctccac tttatccatc agacgcttaa taaggatttt ttccgcgcgg
    16261 aaaccgttac ggcggtgaat cagatgcact tccgaagcga tgttagacag atacagcgcc
    16321 tcttcaaccg cggtattgcc gccgccgatg accgcaactt tctggttgcg atagaagaaa
    16381 ccgtcgcagg ttgcacaagc agaaacccca cggcctttaa aggcttcttc agatggcagg
    16441 ccgagatagc gtgcagaagc tccggtggca ataatcagcg cgtcgcaagt gtattcgccg
    16501 ctatcgccag tcagacggaa cggacgattc tgcaaatcaa ctttgttgat gtgatcaaag
    16561 atgatttcag tttcaaattt ggtggcatgt tcgtgcatgc gctccattaa taacggaccg
    16621 gtcagatcgt ttggatcgcc aggccagttt tccacttccg tggtggtggt cagttggccg
    16681 cctttttcca tgccggtaat cagcacaggt tgcaggttgg cgcgcgccgc atagacagca
    16741 gcggtgtatc ccgccgggcc tgaacccagg ataagcagtt tactgtgttt ggtcgtgccc
    16801 atgagatccc catagttgtt ggcagacaat gggcaggatt gtagggaatt tacagacgta
    16861 aaaaaagagt atgacgattt tgttaacaat ttgtgcaatc ggcagcatcg ataagccggt
    16921 caaattctcc cgtcattatc acctctgcta cttaaatttc ccgctttata agccgattaa
    16981 atgatgaata aacgcccctg ttaatgaata tctggcatgt tgtactaaaa atcgatgttt
    17041 tgctttgaca atcccctggt gttttgcgaa aacattcgag gaagaaaaaa aacagtattc
    17101 ttatatgcgc ataaccatgc atgtaaatac catgtttacc gtgctagtga aatctacgta
    17161 tggcgtggac agacgccatt cgtgatgtcg atagctgcca caaggcaacg gtcttctcac
    17221 cgtagaccca ggcattgcgc gccgtgaatc ttcatgattt cggtctatcg tgacgggtag
    17281 cgactctgaa cagtgatgtt tcagggtcag acaggagtag ggaaggaata cagagagaca
    17341 ataataatgg tagatagcaa gaagcgccct ggcaaagatc tcgaccgtat cgatcgtaac
    17401 attcttaatg agttgcaaaa ggatgggcgt atttctaacg tcgagctttc taaacgtgtg
    17461 ggactttccc caacgccgtg ccttgagcgt gtgcgtcggc tggaaagaca agggtttatt
    17521 cagggctata cggcgctgct gaacccccat tatctggatg catcacttct ggtattcgtt
    17581 gagattactc tgaatcgtgg cgcaccggat gtgtttgaac aattcaatac cgctgtacaa
    17641 aaacttgaag aaattcagga gtgtcattta gtatccggtg atttcgacta cctgttgaaa
    17701 acacgcgtgc cggatatgtc agcttaccgt aagttactgg gggaaaccct gctgcgtctg
    17761 cctggcgtca atgacacacg gacatacgtt gttatggaag aagtcaagca gagtaatcgt
    17821 ctggttatta agacgcgcta acacggaaca ggtgcaaaat cggcgtattt tgattacact
    17881 cctgttaatc catacagcaa cagtactggg gtaacctggt actgttgtcc gttttagcat
    17941 cgggcaggaa aagcctgtaa cctggagagc ctttcttgag ccaggaatac actgaagaca
    18001 aagaagtcac attgacaaag ttaagtagcg gccgccgcct tctggaggcg ttgctgatcc
    18061 ttattgtcct gtttgccgtc tggttgatgg ctgccttact aagttttaac ccttcggacc
    18121 ccagctggtc gcaaacggcc tggcatgaac ctatccataa tttaggtggg atgcccggtg
    18181 cgtggttggc agatacgctg ttctttattt ttggcgtgat ggcttacacc attcccgtca
    18241 ttattgttgg cggttgttgg tttgcctggc gtcatcagtc cagcgacgaa tacattgatt
    18301 attttgccgt ttcgctacgc atcattggcg ttttggcgct catccttacc tcctgtggtc
    18361 tggcggcaat caacgctgac gatatctggt attttgcctc cggtggcgtc attggcagct
    18421 tactaagcac tacgctacaa ccactgctac acagtagcgg gggaactatt gcgctgctct
    18481 gcgtttgggc agcgggcctg acgctgttca ccggttggtc atgggtgacc attgctgaaa
    18541 aactcggcgg ctggatttta aatattctta ccttcgccag taatcgtacc cgtcgcgatg
    18601 atacgtgggt cgatgaagat gagtatgaag acgacgaaga gtatgaagat gaaaatcacg
    18661 gcaaacagca tgaatcacgc cgtgcccgta ttcttcgcgg cgcgctagcg cgtcgtaaac
    18721 ggttggcgga aaaattcatt aatccgatgg ggcggcaaac agacgctgcg ttgttctccg
    18781 gtaagcggat ggatgatgac gaagagatta cctacactgc acgcggtgtg gctgccgacc
    18841 ctgacgacgt cctcttttcg ggcaatcgtg caacgcagcc agaatatgac gagtacgatc
    18901 cattattaaa cggtgcgcca attaccgaac ctgttgctgt agcagctgct gctaccacgg
    18961 cgacgcaaag ctgggctgca ccggttgaac ctgtgactca gacgccacct gttgcctctg
    19021 ttgatgttcc acctgcgcaa cctacagtag cctggcagcc tgtaccgggg ccgcaaacgg
    19081 gagaaccggt tattgctcct gcaccggaag gttacccaca gcagccacaa tatgcgcagc
    19141 ctgcagtgca atataatgag ccgctgcaac aaccagtaca gccacagcag ccgtattatg
    19201 cacctgcagc tgaacaacct gcgcaacaac cttactacgc tcctgcggcg gaacaacctg
    19261 ttcagcaacc gtattactcc cctgcgccag aacaaccggt ggcaggtaac gcctggcaag
    19321 ccgaagagca gcaatccact tttgctccac agtctacata ccagactgag caaacttatc
    19381 agcagccagc cgctcaggaa ccgttgtacc aacagccgca acctgttgaa cagcagcctg
    19441 ttgtggagcc tgaacccgtt gtagaagaga caaaacccgc gcgtccgccg ctttactact
    19501 ttgaagaagt ggaagagaag cgagcccgtg aacgtgaaca acttgcggcc tggtatcaac
    19561 cgattccaga accggttaaa gaaccagaac cgatcaaatc ttcgctaaaa gcaccttctg
    19621 ttgcagcagt acctccagtg gaagccgctg ccgccgtttc tccgctggca tctggcgtga
    19681 aaaaagcgac actggcgacg ggggctgccg caaccgttgc cgcgccagtc ttcagtctgg
    19741 caaatagcgc tggaccgcgt ccgcaggtca aagaggggat tggcccccag ttgccacgac
    19801 cgaaacgcat ccgcgtgcca actcgtcgtg aactggcgtc ttacggtatt aagctgccct
    19861 cacagcgtgc ggcagaagaa aaagcccgtg aagcccagcg caatcagtat gattctggcg
    19921 atcagtataa cgatgatgaa attgatgcga tgcagcagga tgaactggcg cgtcaattcg
    19981 cccagacaca gcagcaacgc tacggcgaac agtatcaaca tgatgtgccc gtgaacgcag
    20041 aagatgcaga tgctgcggca gaggctgaac tggctcgtca gtttgctcaa actcaacaac
    20101 aacgttattc cggcgaacaa cctgctgggg cgaatccgtt cacgctggat gattttgaat
    20161 tttcgccaat gaaagctttg ctggatgatg gtccacacga accgctgttt acgccaattg
    20221 ttgaacctgt acagcagccg caacaaccgg tagcaccgca gcagcaatat cagcagccgc
    20281 aacaaccggt agcaccgcag cagcaatatc agcagccgca acaaccggtt gcaccgcagc
    20341 cgcaatatca acagccgcaa caaccggttg caccgcagca gcaatatcag cagccacaac
    20401 agccggttgc acagcagccg cagtatcagc agccacaaca gccggttaca cagcagccgc
    20461 agtatcagca gccgcaacag ccagttgcgc cacagccgca ggataccctg cttcatccgc
    20521 tgttgatgcg taatggcgac agccgtccgt tgcataaacc gacgacgcca ctgccttctc
    20581 tggatttgct gacaccgccg ccgagcgaag tggagccggt agataccttt gcgcttgaac
    20641 aaatggcgcg cctggtagaa gcgcgtctgg ctgatttccg tattaaagcc gatgttgtca
    20701 attactctcc ggggccagtt atcactcgct ttgaacttaa cctggcaccg ggcgtaaaag
    20761 cggcgcgcat ttctaacttg tcacgggacc ttgcccgttc actttcgacg gtggcggtgc
    20821 gtgtcgttga agttattcct ggcaaaccct atgtaggtct ggagttaccg aataaaaaac
    20881 gacaaaccgt ttatctgcgc gaagttttgg ataacgccaa attccgcgat aatccgtcgc
    20941 cattaaccgt ggtgctgggt aaagatatcg ccggtgagcc ggtggttgcc gatctggcga
    21001 aaatgccgca cttgttggtt gcggggacta ccggttccgg taagtctgtc ggtgtgaacg
    21061 cgatgatcct gagcatgctt tataaagcac agccagaaga tgtgcgtttc atcatgatcg
    21121 acccgaaaat gctggagctt tcggtttatg aaggcattcc gcatctgtta acggaagtcg
    21181 ttactgatat gaaagatgcc gccaacgcgc tgcgctggtg tgttaacgag atggagcgtc
    21241 gttataaact gatgtctgcg ctgggtgtgc gtaatctggc gggttataac gaaaaaattg
    21301 ctgaagccga tcgcatgatg cgtccgattc cagacccgta ctggaagccg ggtgatagta
    21361 tggatgcgca gcatccggtg ctgaaaaaag aaccgtacat agtggtgttg gtggacgaat
    21421 ttgccgacct gatgatgacg gtaggtaaaa aagtggaaga gctgatagct cgtctggcgc
    21481 aaaaagcccg tgccgcgggt atccacctcg tactggcaac gcagcgtcca tcggttgatg
    21541 ttattactgg tctgattaaa gcgaatattc cgacccgtat cgcctttacc gtatccagta
    21601 agattgactc acgtaccatt cttgatcagg ctggcgcgga atcactgctg ggtatggggg
    21661 atatgctcta ctctgggccg aactccacgt tgccggtacg tgtccatggt gcttttgttc
    21721 gcgatcagga agttcatgcc gtggtgcagg actggaaagc gcgtggtcgc ccacagtatg
    21781 ttgacggcat cacctccgac agcgaaagcg aaggtggtgc gggtggtttc gatggcgctg
    21841 aagaactgga tccgttgttc gatcaggcgg tgcagtttgt cactgaaaaa cgcaaagcgt
    21901 caatttcagg cgtacagcgt cagttccgca ttggttataa ccgtgcagcg cgtattatcg
    21961 aacagatgga agcgcaaggg attgtcagcg aacaggggca caacggtaat cgtgaagtgc
    22021 tggccccacc gccgtttgac taattaatgc atcgtagtcc ggataaggcg cggtagcgtc
    22081 gcatccggca ctctaacaac tgaaaattca gtattttctt ctttcctcaa gctgattatt
    22141 agcctggaat agagagtaga gggaactccc gatcgggagt gacgtaattt gaggaataat
    22201 gatgaaaaaa attgccatca cctgtgcatt actctcaagc ttagtagcaa gcagcgtttg
    22261 ggctgatgcc gcaagcgatc ttaaaagccg cctggataaa gtcagcagct tccacgccag
    22321 cttcacacaa aaagtgactg acggtagcgg cgcggcggtg caggaaggcc agggcgatct
    22381 gtgggtgaaa cgtccaaatt tattcaactg gcatatgact caacctgatg aaagcattct
    22441 ggtttctgac ggtaaaacac tgtggttcta taacccgttc gttgagcaag ctacggcaac
    22501 ctggctgaaa gatgccaccg gtaatacgcc gtttatgctg attgcccgca accagtccag
    22561 cgactggcag cagtacaata tcaaacagaa tggcgatgac tttgtcctga cgccgaaagc
    22621 cagcaatggc aatctgaagc agttcaccat taacgtggga cgtgatggca caatccatca
    22681 gtttagcgcg gtggagcagg acgatcagcg cagcagttat cagctgaaat cccagcaaaa
    22741 tggggctgtg gatgcagcga aatttacctt caccccgccg caaggcgtca cggtagatga
    22801 tcaacgtaag tagaggcacc tgagtgagca atctgtcgct cgatttttcg gataatactt
    22861 ttcaacctct ggccgcgcgt atgcggccag aaaatttagc acagtatatc ggccagcaac
    22921 atttgctggc tgcggggaag ccgttgccgc gcgctatcga agccgggcat ttgcattcta
    22981 tgatcctctg ggggccaccg ggtaccggca aaacaaccct cgctgaagtg attgcccgct
    23041 atgcgaacgc tgatgtggaa cgtatttctg ctgtcacctc tggcgtgaaa gagattcgcg
    23101 aggcgattga gcgcgctcgg caaaaccgca atgcaggtcg ccgcactatt ctttttgttg
    23161 acgaagttca ccgtttcaac aaaagccagc aggatgcatt tctgccacat attgaagacg
    23221 gcaccatcac ttttattggc gcaaccactg aaaacccgtc gtttgaactt aactcggcac
    23281 tgctttcccg tgcccgtgtc tatctgctga aatccctgag tacagaggat attgagcagg
    23341 tgcttactca ggcgatggaa gacaaaaccc gtggctatgg tggtcaggat attgttctgc
    23401 cagatgaaac acgacgtgcc attgctgaac tggtgaatgg cgacgcgcgc cgggcgttaa
    23461 atacgctgga aatgatggcg gatatggccg aagtcgatga tagcggtaag cgggtcctga
    23521 agcctgaatt actgaccgaa atcgccggtg aacgtagcgc ccgctttgat aacaaaggag
    23581 atcgctttta cgatctgatt tccgcactgc ataagtcggt acgtggtagc gcacccgatg
    23641 cggcgctgta ctggtatgcg cgaattatta ccgctggtgg cgatccgtta tatgtcgcgc
    23701 gtcgctgtct ggcgattgcg tctgaagacg tcggtaatgc cgatccacgg gcgatgcagg
    23761 tggcaattgc ggcctgggat tgctttactc gcgttggccc ggcggaaggt gaacgcgcca
    23821 ttgctcaggc gattgtttac ctggcttgcg cgccaaaaag caacgctgtc tacactgcgt
    23881 ttaaagccgc gctggcggat gcacgcgaac gtccggatta tgacgtgccg gttcatttgc
    23941 gtaatgcgcc gacgaaatta atgaaggaaa tgggctacgg gcaggaatat cgttacgctc
    24001 atgatgaagc aaacgcttat gctgccggtg aggtttattt cccgccggaa atagcacaaa
    24061 cacgctatta tttcccgaca aacaggggcc ttgaaggcaa gattggcgaa aagctcgcct
    24121 ggctggctga acaggatcaa aatagcccca taaaacgcta ccgttaatgt tatcgttgcg
    24181 gtaatgttgt tactgtatcc ctgtggtcgc aggctgtggc cacatctccc atttaattcg
    24241 ataagcacag gataagcatg ctcgatccca atctgctgcg taatgagcca gacgcagtcg
    24301 ctgaaaaact ggcacgccgg ggctttaagc tggatgtaga taagctgggc gctcttgaag
    24361 agcgtcgtaa agtattgcag gtcaaaacgg aaaacctgca agcggagcgt aactcccgat
    24421 cgaaatccat tggccaggcg aaagcgcgcg gggaagatat cgagccttta cgtctggaag
    24481 tgaacaaact gggcgaagag ctggatgcag caaaagccga gctggatgct ttacaggctg
    24541 aaattcgcga tatcgcgctg accatcccta acctgcctgc agatgaagtg ccggtaggta
    24601 aagacgaaaa tgacaacgtt gaagtcagcc gctggggtac cccgcgtgag tttgactttg
    24661 aagttcgtga ccatgtgacg ctgggtgaaa tgcactctgg cctcgacttt gcagccgcag
    24721 ttaagttgac cggttcccgc tttgtggtaa tgaaagggca gattgctcgc atgcaccgcg
    24781 cactgtcgca gtttatgctg gatctgcata ccgaacagca tggctacagt gagaactatg
    24841 ttccgtacct ggttaaccag gacacgctgt acggtacggg tcagctgccg aaatttgctg
    24901 gcgatctgtt ccatactcgt ccgctggaag aagaagcaga caccagtaac tatgcgctga
    24961 tcccaacggc agaagttccg ctgaccaacc tggtgcgcgg tgaaatcatc gatgaagatg
    25021 atctgccaat taagatgacc gcccacaccc catgcttccg ttctgaagcc ggctcatatg
    25081 gtcgtgacac ccgtggtctg atccgtatgc accagttcga caaagttgaa atggtgcaga
    25141 tcgtgcgccc ggaagactca atggcggcgc tggaagagat gactggtcat gcagaaaaag
    25201 tcctgcagtt gctgggcctg ccgtaccgta aaatcatcct ttgcactggc gacatgggct
    25261 ttggcgcttg caaaacttac gacctggaag tatggatccc ggcacagaac acctaccgtg
    25321 agatctcttc ctgctccaac gtttgggatt tccaggcacg tcgtatgcag gcacgttgcc
    25381 gcagcaagtc ggacaagaaa acccgtctgg ttcataccct gaacggttct ggtctggctg
    25441 ttggtcgtac gctggttgca gtaatggaaa actatcagca ggctgatggt cgtattgaag
    25501 taccagaagt tctgcgtccg tatatgaacg gactggaata tattggctaa tacccaattt
    25561 ttctgaatct aaaaagcgcc tgcgggcgct ttttttgtct ccctttgata ccgaacaata
    25621 attactcctc acttacacgt aatactactt tcgagtgaaa atctacctat ctctttgatt
    25681 ttcaaattat tcgatgtata caagcctata tagcgaactg ctatagaaat aattacacaa
    25741 tacggtttgt tactggaatc aatcgtgagc aagcttgagt gagccattat gaaaacgaaa
    25801 atccctgatg cggtattggc tgctgaggtg agtcgccgtg gtttggtaaa aacgacagcg
    25861 atcggcggcc tggcaatggc aagcagcgcg ttaacattac cttttagtcg gattgcgcac
    25921 gctgttgata gtgcccttcc ggcaaaatca gacgaaaagg ttatctggag cgcctgtaca
    25981 gttaactgtg gtagtcgctg cccgctacgt atgcacgttg tggacggtga aattaaatat
    26041 gttgaaacgg ataataccgg cgatgacaac tatgacggcc tgcaccaggt gcgcgcctgt
    26101 ctgcgtggtc gttccatgcg tcgccgtgtc tacaatccgg accgcctgaa atatccgatg
    26161 aaacgagtcg gggcgcgcgg tgaaggcaaa ttcgagcgca ttagctggga agaagcctac
    26221 gacatcatcg cgaccaatat gcagcgcctg atcaaagagt acggcaacga gtctatctat
    26281 ctgaactatg gtaccggaac gctgggcggc accatgaccc gctcctggcc gccgggaaat
    26341 accctggtcg ctcgcctgat gaactgctgc ggcggttatc tgaaccatta cggtgactac
    26401 tcttccgcgc aaattgcgga aggtttgaac tatacctacg gcggctgggc agatggtaac
    26461 agcccgtcgg atatcgaaaa cagtaaactg gtagtgctgt ttggtaataa ccctggcgaa
    26521 acgcgaatga gtggcggtgg ggtgacttac tatcttgaac aggcgcgcca gaaatctaat
    26581 gctcgcatga tcatcatcga tccgcgctat accgacaccg gtgccgggcg cgaagatgag
    26641 tggatcccta ttcgtccggg aacagatgcc gcactggtta acggtctggc gtatgtcatg
    26701 atcaccgaaa acctggtgga tcaggcattc ctcgataaat attgcgttgg ctacgacgag
    26761 aaaacactgc cagccagtgc gccgaaaaat ggtcactata aagcttatat tctgggtgaa
    26821 gggccagatg gcgtggctaa aacgccggaa tgggcctcgc aaatcactgg tgttccggca
    26881 gacaaaatca tcaaactggc tcgtgaaatc ggtagtacca aaccggcgtt tatcagccag
    26941 ggatggggcc cgcagcgtca cgctaacggt gaaatcgcaa cccgtgctat ctcgatgctg
    27001 gcgattctga ccggtaacgt tggtattaac ggaggcaaca gcggcgcgcg tgaaggttca
    27061 tacagcttac cgtttgtccg tatgccgacc ctcgaaaacc cgatccagac cagcatttcg
    27121 atgtttatgt ggaccgatgc cattgaacgt ggcccggaaa tgacagcgct gcgtgatggt
    27181 gtgcgcggga aagataagct ggatgtgccg atcaaaatga tctggaacta tgccggtaac
    27241 tgcctgatta atcagcattc tgaaatcaac cgtacccatg aaatccttca ggatgataag
    27301 aagtgcgagc tgattgtggt tatcgactgc cacatgacct catcggcgaa atatgctgac
    27361 atcctgctgc ctgactgcac cgcttcagaa cagatggact ttgcactgga tgcatcctgc
    27421 gggaatatgt cttacgtgat tttcaacgat caggtgatta aaccgcgctt tgaatgtaag
    27481 accatctatg aaatgaccag cgaactggca aaacgtcttg gcgttgagca acagtttact
    27541 gagggccgta cccaggaaga gtggatgcgg catctgtatg cccagtcgcg ggaagcgatt
    27601 cctgaactgc caacgtttga agagttccgt aagcagggga tctttaaaaa gcgcgaccca
    27661 caagggcatc acgttgctta taaagctttc cgtgaagatc cgcaggcaaa tccactgacc
    27721 acgccatcgg ggaaaattga gatttattcg caggcgctgg ctgacattgc cgctacctgg
    27781 gaattgccag aaggtgatgt gatcgatcca ctgccgatct acacgccggg ctttgaaagt
    27841 tatcaggatc cgctgaacaa acagtatccg ctgcagctta cgggtttcca ctataaatct
    27901 cgcgttcact caacttacgg caacgttgat gtgctgaaag cagcttgccg tcaggaaatg
    27961 tggatcaacc cgcttgatgc gcaaaaacgc ggtatcaaca acggcgataa agtcaggatc
    28021 tttaacgatc gtggtgaggt tcatattgag gcgaaagtga cgccacgaat gatgccgggt
    28081 gtggtcgcac tgggtgaagg tgcctggtat gacccggata caaaacgtgt cgataagggc
    28141 ggttgtatca acgtactgac cactcaacgt ccgtctcctc tcgctaaggg gaatccgtca
    28201 catacaaacc tggttcaggt tgaaaaggtg taaggagtaa ccgatgacaa cccagtatgg
    28261 attttttatt gattccagcc gttgcaccgg ttgcaaaacc tgcgagctgg cttgtaaaga
    28321 ctacaaagat ttgacgccag aagtcagttt ccgccgcatt tatgaatatg ctggcggcga
    28381 ctggcaggaa gataacggtg tctggcacca gaacgtgttt gcctactatc tgtcgatttc
    28441 atgtaaccac tgcgaagatc cggcttgtac taaagtctgc ccgagcggtg cgatgcataa
    28501 acgtgaagat ggttttgttg tggtcgatga agatgtgtgc attggctgcc gttactgcca
    28561 tatggcttgc ccgtacggcg caccgcaata taacgaaacg aaaggccata tgaccaaatg
    28621 cgatggttgt tatgaccgtg ttgcggaggg gaaaaagccg atctgtgttg aatcttgccc
    28681 gctgcgggcg ctggatttcg gccctatcga cgagctgcgt aaaaaacatg gcgatctggc
    28741 ggccgttgcg ccgttgccgc gagctcactt taccaaaccg aatattgtga tcaaacccaa
    28801 tgccaacagc cgcccgaccg gggataccac tggctatctg gcaaacccga aggaggtgtg
    28861 agatgggaag tggatggcat gaatggccgc tgatgatctt cacggtcttc gggcaatgtg
    28921 tagcgggtgg ttttatcgtt ctggctttgg cgctgctcaa aggcgacctg cgagcagaag
    28981 cccagcagcg tgttattgcc tgcatgtttg gtttatgggt gctgatgggc attggcttta
    29041 tcgcctctat gcttcatctt ggttcaccga tgcgcgcctt taactcgctc aaccgggtag
    29101 gggcttcagc actcagtaac gaaatcgcca gcggttcgat cttttttgct gttggtggca
    29161 tcggctggtt gctggcaatg ctgaaaaagc tgtcaccggc attgcgtacg ctgtggctga
    29221 tagtgacgat ggttcttggc gtcatctttg tctggatgat ggtgcgtgtg tataacagca
    29281 ttgataccgt tccgacctgg tacagcatct ggacgccgat gggcttcttc ctgacgatgt
    29341 ttatgggcgg cccgttactg ggttacctgt tgttgagtct ggctggcgtc gatggctggg
    29401 cgatgcgtct gctgccagcc atttctgtac tggcactggt agtgagtggc gtgatgtcag
    29461 tgatgcaggg cgcagagctg gcgactattc atagttctgt gcagcaggcc gcagcgctgg
    29521 tgccggacta tggtgcgctg atgtcctggc ggatcgtgct tttggctgtt gccctgtgct
    29581 tgtggattgc accacagcta aaaggttatc agcctgcggt tccgctactt tccgtatcat
    29641 tcattctgct tctggcaggg gaattaatcg gtcgtggcgt attctatggt ttgcatatga
    29701 ccgtggggat ggccgtcgca agctaatcat aacaaccggg gtttcggccc cggttctctt
    29761 ttatttctgc ttcgttaacg tgtcataact ggtcatcaaa ttacgataat caggaatatg
    29821 gttagagaac aacgtcgcca gtccttcaat atcattacgc cagtcgcgat gcagctcgca
    29881 cgccacgccg aaccatgtca tcaattgcgc accagcttgc gacatgcgat cccatgccga
    29941 atgccgggta atttcattaa aggtccctga tgcgtcagtc acgacaaaca catcaaaccc
    30001 ctcttcaatg gctgaaagcg ccgggaatgc cacgcaaact tcggttacca caccggcaat
    30061 aattaactgt tttttacctg tcgctttgac tgcttttaca aaatcttcgt tatcccaggc
    30121 gttaatattt cccgggcgag caatgtaagg tgcatcgggg aattgtgctt tcagttctgg
    30181 aactaatggg ccgttaggac cggtttcaaa actggtggta agaatagttg gtaagttgaa
    30241 atacttagcc aggtcaccca gcgccagcac gttattttta aacttatcgg gttcgatatc
    30301 ccgtacaagg gaaagtaaac cagcctggtg atcaacaagc aaaacggcag catcattttt
    30361 atcaagacga acatacggtt tggtcatcct cttctccttt cgagagtcag tggtacgtct
    30421 gaaatcggtc agacgcgatg cattgctctg aaagcataga cgggaaatat gagtttgctg
    30481 tgaccatgaa atttttcgac tgaacgaagc gtatgaaatt tgtgttagat caataaaaac
    30541 aatcagatga gattaatcgt ctctccagca ttagcgttat gaatgagtaa tataaatcga
    30601 taatacctcc gctgcgatat gcgcgtattt tcacccattg acaatgtttt tggcggtggc
    30661 atgatgcgca tgaaatttga acttcctcac ggttttaatt catgtccacg tatacccggc
    30721 ctgtcatgct tttgctgtct ggcctgcttt tgttgactct ggcgatcgca gtgttaaata
    30781 cactcgtgcc gctttggctc gcccaggaac acatgtccac atggcaggta ggcgttgtca
    30841 gctcatccta ttttaccggc aacctggtcg gtacattgct gacagggtat gtcattaagc
    30901 gcattggctt taaccgcagc tattatctgg cctccttcat ttttgccgct ggctgtgccg
    30961 gccttggcct gatgattgga ttctggagct ggttggcttg gcgttttgtc gccggtgtcg
    31021 gttgtgccat gatttgggtg gttgttgaga gcgcgctgat gtgcagtggg acgtcacgta
    31081 accgtgggcg tttgcttgct gcgtatatga tggtttatta cgtgggaacg tttttaggcc
    31141 agttactggt cagcaaagtt tcaaccgagc tgatgtccgt attgccgtgg gttacaggtt
    31201 tgacgttggc agggatctta ccgctgttgt ttacgcgtgt gctgaatcag caggctgaaa
    31261 accatgattc gacgtcaatt acgtcaatgc taaaactccg tcaggcgcgg cttggcgtga
    31321 atggctgcat tatctcagga atcgtgctgg gatctctata tggcctgatg ccgctgtacc
    31381 tcaatcacaa aggggtgagc aatgccagca ttggtttctg gatggcggta ctggtcagtg
    31441 cgggtatcct cggacaatgg ccgatcggac gtctggcgga taagtttggt cgactgctgg
    31501 tgttgcgtgt tcaggtcttt gtcgtcattc tcggcagtat cgcgatgctt agccaggcgg
    31561 cgatggcccc agcgttattc atcctcggtg ccgctggctt tacgctatat ccggtggcga
    31621 tggcctgggc ttgtgagaaa gttgaacatc atcaactggt ggcgatgaac caggccttac
    31681 tgttgagcta taccgtggga agtctgcttg gcccgtcatt taccgctatg ctaatgcaga
    31741 atttctccga taatttattg tttatcatga tcgccagcgt atcgtttatc tatttgctga
    31801 tgctgcttcg caacgccggt catacgccga aacccgttgc tcacgtgtaa atgaattcaa
    31861 gcagagtgtg aacttactgt ttcacactct gcttttttgt ttcttctatc tgacttgctt
    31921 tattccaaat tttattcgtt taaaaataaa atgtgcagca ggttataatt ttgcatttcg
    31981 ctatttccgc acttcttatt tgccgcgcat aatccctcgt tttacagatg cccctttaat
    32041 tttggcgaag gatttgtcta tggctgggaa tgttcaggaa aaacagctgc gatggtacaa
    32101 cattgcgctg atgtctttta tcactgtctg gggttttggc aatgttgtta ataactacgc
    32161 caaccagggg ctggtggttg ttttttcatg ggtgtttatc tttgcgctct atttcacacc
    32221 ttatgcgcta attgttggtc agttaggctc gaccttcaaa gatgggaagg gcggggtcag
    32281 tacctggatt aaacacacga tgggacccgg actggcttac ctcgccgcgt ggacctactg
    32341 ggtagtgcat attccctatc tggcacaaaa accccaggca atactgattg cgctcggttg
    32401 ggcgatgaaa ggcgacggtt cgttaatcaa agaatattca gtcgtagcgt tacaggggtt
    32461 aacgctggtg ctgtttatct tctttatgtg ggttgcttca cgcggtatga aatcgctgaa
    32521 aatcgtcggt tctgtggcag ggattgcgat gtttgttatg tcgctcctgt atgtggcgat
    32581 ggcggtaacc gcgcctgcaa ttactgaagt gcatattgcg accacaaaca ttacctggga
    32641 aacgttcatt cctcatatcg actttaccta cattaccact atttcaatgc tggttttcgc
    32701 ggttggcgga gcagagaaga tttctcctta cgttaatcaa acgcgcaacc caggaaaaga
    32761 atttccaaaa gggatgttat gcctggcggt gatggttgcg gtttgtgcca ttctgggttc
    32821 actggcgatg gggatgatgt ttgactcgcg taatatcccg gatgacttaa tgactaacgg
    32881 tcagtattac gcctttcaga agctgggcga gtattacaac atgggtaata ctttaatggt
    32941 gatttacgcc attgcgaata ccctgggaca agtagctgcg ctggtattct cgattgatgc
    33001 cccgcttaaa gtgctattag gcgatgctga cagcaaatat attccagcca gtttatgtcg
    33061 taccaacgct tctggtacgc ccgttaatgg ctattttctg accctggtgc tggtggcgat
    33121 cctgataatg ctaccgactc tcggcattgg tgatatgaac aatctctata aatggctgtt
    33181 gaatcttaac tcggtagtga tgccgctgcg ttatttatgg gtatttgttg catttattgc
    33241 agttgttcgc ctggcgcaga aatataagcc ggaatatgtc tttattcgta ataagccgct
    33301 ggcaatgacc gtcggaattt ggtgttttgc ctttaccgct tttgcctgcc tgactggtat
    33361 tttcccgaaa atggaagcct tcaccgcaga gtggacattc cagttggcgc tgaatgttgc
    33421 aacgccgttt gtgctggtag gattagggct gatattcccg ctgctggcgc gtaaagcgaa
    33481 tagcaaataa ttattgtggt cggtgacgct gactctggca gatctgaccc gtttttacat
    33541 aatcgagaaa tgcgcgcaag ccgcaggaca tatactggcg gtttgggtaa tacatttgga
    33601 agctgtgctg taagggcgtt agaaatagag ctctgaatta tttatgcagc gcggaataaa
    33661 aaagcggtaa ctcaataaag ttgccgcttt acggggaaat tagaacatga ccttatgacc
    33721 gtactgctca agaatgcctt tcacgcgttc catggtctct ttcttcggtg gtttaacacc
    33781 atcgagtttg tattcttcac ccatcgccac ccatttgtgt ttgcccagtt cgtggtaggg
    33841 gagaagttcg attttctcaa cgttgcccat atcacgggta aattcaccaa ggcgatgcgc
    33901 tgaatcgtca tcgtcagacc agcctgggac aacaacatag cggatccaca ccttcacatt
    33961 tttgttcgcc agatatttag cgaactccag cgtgcggtgg ttggaaactc caaccagatt
    34021 ttggtggatc tcgtcgttca tctgtttgag atcgagcatt accaggtcgg ttacttccag
    34081 cagttcatca atcaccggat cgtaacgacg aacaaaaccg ttggtgtcca gacaggtatg
    34141 aatgccttct tttttgcagg cgcggaacca gtcacgaaca aactcagctt gtaggattgc
    34201 ctcaccgccg gatgcggtaa cgccgccgcc ggaagcgttc ataaagtggc gataggtcac
    34261 cacttccttc atcaaatctt caacggtaac ttctttaccg ccatgcgtat cccaggtgtc
    34321 gcggttatga caatacaggc agcgcatcag gcagccctgg aaaaaggtga taaagcgaat
    34381 acccgggccg tctacggttc cacaggattc aaaggagtga atgcgaccaa taactgacat
    34441 tgcggtgttt ctccagatgt ggcccatctg aggccgtgtt ggtgcgcagc tcgaaggcta
    34501 cgtcgagtct gttttggcag tcaccttaaa gtatagatag ctgacaaaaa aggctctcgt
    34561 gctaaaaaag gccccacttt cgtggagcct ttattgtacg ctttttactg tacgatttca
    34621 gtcaaatcta attacataga ttgagtgaag gtacgagtaa taacgtcctg ctgctgttct
    34681 ttagtcagcg agttgaaacg tactgcgtag ccagatacac ggatggtcag ctgcggatat
    34741 ttttccgggt tttccatcgc gtcgagcagc atttcacggt tcatcacgtt aacgttcagg
    34801 tgctgaccac cttcgatgga tgcttcgtgg tggaagtaac catccatcag accagccagg
    34861 ttggtcttac gaacttcgtc gtctttaccc agtgcgttcg gaacgataga gaaggtgtag
    34921 gagataccat ctttagcgta agcaaacggc agtttagcaa cggaagtcag agacgctaca
    34981 gcacctttct ggtcacgacc gtgcatcggg ttagcacccg gtccgaacgg cgcgccagca
    35041 cgacgaccgt ctggggtgtt accagttttc ttaccataca caacgttaga agtgatggtc
    35101 agaacagact gagtcgggat agcgtcacgg taggtgtgca gtttctgaat tttcttcatg
    35161 aaacgttcta ccaggtcaac agccaggtca tctacacgcg gatcgttgtt accaaactgc
    35221 gggtattcgc cttcgatttc gaagtcgata gccagaccgt cttcatcacg aatcggttta
    35281 actttcgcat atttgattgc agacagggag tcagcagcaa cggacagacc agcgatacca
    35341 cacgccatgg tgcggataac gtcacggtcg tgcagcgcca tcagagaggc ttcgtagctg
    35401 tacttgtcgt gcatgtagtg gatgatgttc agtgcagtga tgtactgttt agccagccag
    35461 tccatgaagt gatccatgcg ctccatcact tcatcatagt tcaggacgtc gcctttgatc
    35521 ggttcagact tcggaccaac ctgcattttc agtttttcgt caacgccgcc gttgattgcg
    35581 tacagcatgg ttttcgccag gtttgcacgc gcaccgaaga actgcatttg tttaccaacg
    35641 atcatcgggc ttacgcagca agcaatagcg tagtcatcgt tgttgaagtc cggacgcatc
    35701 aggtcatcgt tctcatactg cagagaagag gtgtcgatgg acactttagc ggcgaatttc
    35761 ttgaagttca gtggcagttt ttcagaccac agaatggtca tgttcggttc cggagacgga
    35821 cccatggtgt acagggtgtt caggaaacgg aagctgtttt tggtaaccag ggtacgaccg
    35881 tcgaggccca taccaccgat agattcggtt gcccagatcg ggtcgccaga gaacagttca
    35941 tcgtattccg gagtacgcag gaagcgaacc atacgcagtt tcatgaccag gtggtcaacc
    36001 atttcctgcg cttcttgttc ggtgatcttg ccagctttca ggtcacgttc gatgtacaca
    36061 tccaggaagg tggaggtacg accgaaggac attgcagcac cgttctgaga cttaacagca
    36121 gccaggtagc cgaagtaagt ccactggata gcttcctgag cgttggtagc cggaccagag
    36181 atgtcgtagc cgtatttcgc agccatttct ttcatctgac ccagagcgcg gtgctgttca
    36241 gcgatttctt cgcgcagacg gatagtctgt tccaggttta cgccgttttc cagatcagcc
    36301 tgcagagagg tgaactgtgc aagtttgtct ttcatcaggt agtcgatacc gtacagcgca
    36361 acgcgacggt agtcaccgat gatacgacca cggccataag catctggcag accggtcaga
    36421 acaccggatt tacggcaacg caggatgtcc ggagtgtaaa cgtcgaacac gccctggttg
    36481 tgagttttac ggtattcagt gaagattttt ttgatcatcg ggtccagttc gcggttgtac
    36541 gctttgcagg aaccttcgat cattttgata ccaccgaacg ggataagagc acgtttcagc
    36601 ggagcttcag tctgcagacc aacgattttc tcaagctgct tgttgatgta accagcgtcg
    36661 tgagaggtga tggtggaagc aacagcggtg tcaaagtcaa ctggcgcgtg agtgcggttt
    36721 tccagtttaa cgccttccat tactttgtcc cacagggtgg tggtcgcttc agtagcgcca
    36781 gccaggaagg actcgtcacc ctcgtacgga gtgtagtttt tctgaatgaa gtcacggacg
    36841 tttacttcat tctgccagtc acctttggta aaaccttccc aggctgtggc taacttttca
    36901 ttaagctcgg acatgtaaca cctaccttct taagtggatt ttttatttac tgcgtacttc
    36961 gacaaccatt aatggtgatc gttttcacgc aggtaaatga cccagtatgt caacccaacc
    37021 aacaaaccac cgccgataat attaccgatc gtaaccggaa tcaggttatc agtgatgaaa
    37081 ttcatcacgg tcaggtgaga aaaattttcc ggtgcagaac cgacagcggt ccagaattcc
    37141 ggagatgcga agtcgcggat tacaataccc atcgggatca taaacatgtt tgcgatactg
    37201 tgctcaaaac cgctggcaac aaacatcgcg accggcagca ccataatgaa cgctttgtcc
    37261 atcaggctgc ggccagaata actcatccat actgccagac ataccatcag gttcgccagg
    37321 ataccaagac agacggcctc aataaaagta tggtgcactt tgtggtcggc ggtttgtagg
    37381 acgtttagtc cccattgacc atttgcggtc atatactcgc cggaaagcca cattaaaagt
    37441 acaaacagca gtgcgccgac caggttgcca aaataaacat ttagccagtt tttcgccaac
    37501 tgaccccagg tgatgcgccc actcgccttg gcaacaacaa tcaacactgt ggaagtaaag
    37561 agatcggctc cgcagacaac acaaagaatc aaccccagag agaagcaaat gccgccaacc
    37621 agttttgcca tgccgaaggg cattgtacct gtgccagtgg ttgctgtgat atagaagacg
    37681 aatgcgattg agatgaaaac accggcggta atcgccagat agaaagtctt aagcggatgt
    37741 ttcgttgctt tatagacacc tgcctcttcg gccactttgg ccattgcagc aggaagtaaa
    37801 agatcaaaag ggttgtcagc tttcacacta actctctctt tattaagtcg gcgacgagat
    37861 actaacaaag cattatagat gagaaattga tatagatcat atctcgcctg gcttataggc
    37921 ccgtaactcg catggttttt atgcaaatac ggagtaaata t
//
LOCUS       DAPHCV010000039        37877 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.039, whole genome shotgun sequence.
ACCESSION   DAPHCV010000039 DAPHCV010000000
VERSION     DAPHCV010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.039"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            98..1009
                     /locus_tag="RH033_001953"
     CDS             98..1009
                     /locus_tag="RH033_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2991141.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNNFFNNIQHAFNKKDWT
                     SNYDSLLTLREFFRCATQIDKNSYQVLSSKNETVNAMDKFLISFSLKDNGAEYIMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYAWDILQKKQSDIPERIKGYIRQ
                     AFGDVSLGYAVLDDIVSKLKRGKFDLQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAVDS
                     DITEEEICEKYKSTIEAFKKKQLALPV"
     gene            complement(1099..1653)
                     /locus_tag="RH033_001954"
     CDS             complement(1099..1653)
                     /locus_tag="RH033_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDV2991142.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            1978..2184
                     /locus_tag="RH033_001955"
     CDS             1978..2184
                     /locus_tag="RH033_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HDV2991143.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(2262..3605)
                     /locus_tag="RH033_001956"
     CDS             complement(2262..3605)
                     /locus_tag="RH033_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDV2991144.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(3928..4566)
                     /gene="msrA"
                     /locus_tag="RH033_001957"
     CDS             complement(3928..4566)
                     /gene="msrA"
                     /locus_tag="RH033_001957"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDV2991145.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRRITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            4772..6505
                     /gene="tamA"
                     /locus_tag="RH033_001958"
     CDS             4772..6505
                     /gene="tamA"
                     /locus_tag="RH033_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HDV2991146.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGELEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYSHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSFDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            6502..10281
                     /gene="tamB"
                     /locus_tag="RH033_001959"
     CDS             6502..10281
                     /gene="tamB"
                     /locus_tag="RH033_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HDV2991147.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITMTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEAAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVSTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGLFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHELPESAVGVSSDVVMLNDNLQPEEAKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTEGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            10284..10625
                     /locus_tag="RH033_001960"
     CDS             10284..10625
                     /locus_tag="RH033_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HDV2991148.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(11005..11535)
                     /gene="ppa"
                     /locus_tag="RH033_001961"
     CDS             complement(11005..11535)
                     /gene="ppa"
                     /locus_tag="RH033_001961"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HDV2991149.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            11845..12801
                     /gene="ytfQ"
                     /locus_tag="RH033_001962"
     CDS             11845..12801
                     /gene="ytfQ"
                     /locus_tag="RH033_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HDV2991150.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSILYLPDTAKEELEKKKNMGY"
     gene            12941..14443
                     /locus_tag="RH033_001963"
     CDS             12941..14443
                     /locus_tag="RH033_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDV2991151.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEIEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKLQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            14457..15479
                     /locus_tag="RH033_001964"
     CDS             14457..15479
                     /locus_tag="RH033_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2991152.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RNRDKT"
     gene            15466..16461
                     /gene="yjfF"
                     /locus_tag="RH033_001965"
     CDS             15466..16461
                     /gene="yjfF"
                     /locus_tag="RH033_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HDV2991153.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPVYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(16494..17492)
                     /gene="fbp"
                     /locus_tag="RH033_001966"
     CDS             complement(16494..17492)
                     /gene="fbp"
                     /locus_tag="RH033_001966"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HDV2991154.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            17668..19041
                     /gene="mpl"
                     /locus_tag="RH033_001967"
     CDS             17668..19041
                     /gene="mpl"
                     /locus_tag="RH033_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HDV2991155.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(19199..19750)
                     /locus_tag="RH033_001968"
     CDS             complement(19199..19750)
                     /locus_tag="RH033_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HDV2991156.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIEQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            19844..21196
                     /gene="pmbA"
                     /locus_tag="RH033_001969"
     CDS             19844..21196
                     /gene="pmbA"
                     /locus_tag="RH033_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HDV2991157.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILSQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            21379..21765
                     /gene="cybC"
                     /locus_tag="RH033_001970"
     CDS             21379..21765
                     /gene="cybC"
                     /locus_tag="RH033_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HDV2991158.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(21810..22274)
                     /gene="nrdG"
                     /locus_tag="RH033_001971"
     CDS             complement(21810..22274)
                     /gene="nrdG"
                     /locus_tag="RH033_001971"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HDV2991159.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(22432..24570)
                     /gene="nrdD"
                     /locus_tag="RH033_001972"
     CDS             complement(22432..24570)
                     /gene="nrdD"
                     /locus_tag="RH033_001972"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDV2991160.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRSQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(24955..26610)
                     /gene="treC"
                     /locus_tag="RH033_001973"
     CDS             complement(24955..26610)
                     /gene="treC"
                     /locus_tag="RH033_001973"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDV2991161.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQEINVEAALA
                     DESSVFYTYQKLIALRKQEAVLTWGDYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     TQPWQPGKMLGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            complement(26660..28081)
                     /gene="treB"
                     /locus_tag="RH033_001974"
     CDS             complement(26660..28081)
                     /gene="treB"
                     /locus_tag="RH033_001974"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HDV2991162.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLHFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(28200..29147)
                     /gene="treR"
                     /locus_tag="RH033_001975"
     CDS             complement(28200..29147)
                     /gene="treR"
                     /locus_tag="RH033_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HDV2991163.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            29332..29385
                     /gene="mgtL"
                     /locus_tag="RH033_001976"
     CDS             29332..29385
                     /gene="mgtL"
                     /locus_tag="RH033_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HDV2991164.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            29525..32221
                     /gene="mgtA"
                     /locus_tag="RH033_001977"
     CDS             29525..32221
                     /gene="mgtA"
                     /locus_tag="RH033_001977"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDV2991165.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIVLPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(32427..32813)
                     /gene="ridA"
                     /locus_tag="RH033_001978"
     CDS             complement(32427..32813)
                     /gene="ridA"
                     /locus_tag="RH033_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HDV2991166.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(32886..33347)
                     /gene="pyrI"
                     /locus_tag="RH033_001979"
     CDS             complement(32886..33347)
                     /gene="pyrI"
                     /locus_tag="RH033_001979"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HDV2991167.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(33360..34295)
                     /gene="pyrB"
                     /locus_tag="RH033_001980"
     CDS             complement(33360..34295)
                     /gene="pyrB"
                     /locus_tag="RH033_001980"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HDV2991168.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(34299..34433)
                     /gene="pyrL"
                     /locus_tag="RH033_001981"
     CDS             complement(34299..34433)
                     /gene="pyrL"
                     /locus_tag="RH033_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HDV2991169.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(34714..35109)
                     /locus_tag="RH033_001982"
     CDS             complement(34714..35109)
                     /locus_tag="RH033_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDV2991170.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(35241..35954)
                     /gene="bdcA"
                     /locus_tag="RH033_001983"
     CDS             complement(35241..35954)
                     /gene="bdcA"
                     /locus_tag="RH033_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV2991171.1"
                     /translation="MGAFTGKTVLLLGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            36025..36618
                     /locus_tag="RH033_001984"
     CDS             36025..36618
                     /locus_tag="RH033_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDV2991172.1"
                     /translation="MVTKKQSRVPGRPRRFAPDQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAC
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            36763..37215
                     /gene="tabA"
                     /locus_tag="RH033_001985"
     CDS             36763..37215
                     /gene="tabA"
                     /locus_tag="RH033_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HDV2991173.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKANVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPARVRKAVVKMLMA"
     gene            37338..>37877
                     /locus_tag="RH033_001986"
     CDS             37338..>37877
                     /locus_tag="RH033_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2991174.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARIAGGQD
                     SRRNILDPKTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLSTITTMGTRQGHTAIIFQQSDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANK"
BASE COUNT         9198 a         9336 c        10135 g         9208 t
ORIGIN      
        1 aagttatatt ttaataatga gcgttttttg atagtttact tctataatga gatatttaat
       61 ggcgacataa aataaccaaa taaaataagg tagccatatg ttacccagga tcagacacaa
      121 taattttatt ggtgcggtgg agttattcgt aaaatcatcg catacaaaaa cacattcaaa
      181 caattttttt aacaatatcc agcatgcatt taataaaaaa gattggactt caaattatga
      241 tagcctgtta actttgaggg agttctttcg ttgcgcaact cagattgata aaaatagtta
      301 tcaggtactt tcatcaaaaa atgaaactgt taacgccatg gataagtttc ttatcagttt
      361 cagccttaaa gataacggtg ccgaatatat aatgacattg cgaggcagtg gatttgaata
      421 cgaagaaatt cccataacaa taaatgagta taactcattc atggatttca aaaaccgcga
      481 gtttccgtta gaacaaaaca gaagattgta tgcctgggat attctgcaga aaaaacagtc
      541 tgacataccg gaaaggatta aaggttatat tcgccaggcg tttggtgacg tgtctttagg
      601 ttatgctgta ttggatgata tagtaagtaa attaaaacgg ggtaaatttg acctccaaat
      661 accaggaggt ggaattaaag aatgcgatgg atggtatatt tacgaaaaaa taattgatga
      721 taattttgcc atagtcatag aatctttggg ctttgcgctt aaaatctatg gaggggatga
      781 acgttttcgt aatggttcct ctgttgtttt ggaggatgaa gattactctc ttatttataa
      841 ttttctggtt aatgcaggtt gccaacaagt ggaattagcc gaacaagtgg atgcgatagt
      901 aagtgcgaat ctggccgtag atagtgatat taccgaagag gagatttgcg agaaatataa
      961 atcaaccatt gaggcgttta aaaaaaagca actagcgtta ccagtatgag ttcggcgtaa
     1021 gaactcagag gcttaattcc cctatcccat agataacgat aggggaaaat tatttttgca
     1081 gaatttatgt tattgccatt atttgagcaa cttttgcagc aggtccatca cttgctgcac
     1141 ctcttcctgt gtgagtgccc cgtctttggc ccattgcacg cgaccgtctt tatccagcac
     1201 cactacagcg gaactttctt catccagctg ccaggcaccg cgcgcgacgc cattgctatc
     1261 gacaataaac tgcgaccagg gataaagctt tttattactc tccagactgc tgcgcacaaa
     1321 catgccggaa cccggaatcg cgtcgtcggt gttaacaatg gtggtggtct ggtaacgatc
     1381 gtgcggtaac ttcgctgatt taatcgcttc aatcagcgtc gcgtttttct cttttgcaga
     1441 ggtgcgacca gcaatatgtt gcagtactcg cacttttccc actaactgcg cgctgttcca
     1501 ggttttgtag ctaaactgat ctttatcaag caccaactcg ccacgatcgg tgatgccaat
     1561 cggcggcact cgctgaccgg tttcgaactg atgtgcggaa gccatcatcg gcaacagcag
     1621 gcaggtgagt gccagaatct tgcgtagggt catggtgttt ccttcttatg atatgcaggt
     1681 gatccgacca cttgggtctg agttttaatc atatgtgcta tttatcgaat ttcccgcaag
     1741 tgtgatgcca gtttgcggtc aagcgcacaa atcatatgaa aaatgaatgc ttatactgaa
     1801 gaccgcgctt cggtaaaaag ataattctga ataattgtaa cctttaggta aaaaaagtta
     1861 tacgcggtgg aaacattgcc cggatagtct atagtcacta ggcattaaaa tttgcgcctc
     1921 ataatagttg ggccgattgt ggcaccgcaa aggcgtaata ctcagcagga gataacaatg
     1981 aaaattttcc aacggtacaa cccacttcag gtggcgaagt acgtaaagat cctgttccgt
     2041 ggacggttat acatcaagga cgttggcgct tttgaatttg ataagggtaa gattcttatc
     2101 ccaaaagtga aggataagct gcatttgtca gtgatgtccg aagttaaccg tcaggttatg
     2161 cgtctgcaaa cagagatggc ttaaccaaag tgctatgcag taaaaagtgc tatgcagtaa
     2221 taagacggct cctgattcag gagccgttga tgtttctggg gttacgcgac gctttcttct
     2281 ttatctttag cgtcaggcag ttttggcgaa agggcggtgg ctttgctgtc gattcgggtc
     2341 accagcagct ggtcgatgcg gtagttatcg atatccacca cttcaaattt gtagccggcg
     2401 aatttcaccg aatcggtgcg tttcgggatc ttacgcagca taaacatcat aaagccgccg
     2461 atggtttcgt agttgcccga ctgcgggaac tcgtcaatat ccagcacgcg catgacgtcg
     2521 tcaataggcg tgccgccgtc tatcagccat gagttctcat cacgggcgac aatctgctct
     2581 tccagcccct gaccgaccag atcgcccatc agcgtggtca tcacgtcgtt gagggtgatg
     2641 atccccacta ccagtgcgta ctcgttcatg atcaccgcga agtcttcacc tgcggtttta
     2701 aaactttcca acgcctctga aagggttaac gtatccggca caatcagcgt attgcgaatt
     2761 tgtacgccgc tgttcagtgc caggctttgg ttagccagca cgcggttcag caggtcttta
     2821 gaatcgacat agccgatgat gtggtcaata tcttcattac agacgagaaa cttagagtgc
     2881 ggatgttccg ccaccttatt cttcagactt tgctcatctt cgtggagatc aaaccaaatc
     2941 acgttttcac gcggtgtcat tgaagacgga acggtacggg attccagctc aaagacgttt
     3001 tcaatcagct cgtgttcctg tttacgtaac acgcccgcca gcgcaccggc ttccactacc
     3061 gcgtagatat catcagaagt gatgtcatct ttacgtacca ttggcagttt gaaaatacgg
     3121 aagattatgt tcgccagtcc gttgaagaac cacaccagcg gggtgcaaac gtacaggcag
     3181 aagcgcatcg ggttgatgat acgcaaagcc acagcttctg gcgcaatcat accgatgcgt
     3241 ttcggggtta aatccgcaaa caggataaac atgccagtca ctaacgagaa agagagaata
     3301 aagctcagtt gctcagagag ttctgccgac atatagcggg agaacaggct gtgaaaagct
     3361 ggagaaaatg ccgcatcacc gacgataccg ccgagaatcg ccactgcgtt cagaccgatc
     3421 tggaccacgg taaagaacat gccgggattt tcctgcatat tcagaacgcg ttgggcattt
     3481 atattgcctt catcagccag cagtttaagt ttgattttgc gtgaggcggc aagcgagatc
     3541 tcggacatcg agaagaacgc acttacagcg atcaaacaga gtatgactaa aatactgttt
     3601 aacatatctt atccgacctt tcaggccaga tcctcggaag gggaagttga ttaatttgtg
     3661 tgaaatacac attgaaagcc gattcgaaga aagtgaaccg gcattttcag tggctagtat
     3721 agcgtaaggt actgtaaagc cgccagaggg ttaaaattca gacagctgaa aaatgaaaaa
     3781 ctgcctgatg cgctatgccg aacaggccta cgcagtcccc gcaatatatt gaatttggac
     3841 gattttgtag gccgggcaag gcgtttacgc cgtatccggc ataaacaaaa tgcactttat
     3901 caacaatcta catttgtacc cgtaacgcta tgcttccggc ggcagacaga cgccaattcc
     3961 gccaattcca cagtaaccat acgggttttt atgcagatat tgctggtggt catcttcggc
     4021 ataataaaac ggtgtggcgt tagcgatttc cgtggtgatg cgacgatcgt catcggcggc
     4081 aagcatcgcc gcctgaaaac gttccagact ggcgcgagcg gcggcatcct gttctggggt
     4141 cagcggataa atcgctgaac gatactgcgt gccgtggtca ttgccctgac gcattccctg
     4201 ggcgggatcg tgattctccc aaaatacttg tagcaactgc tcatagctga tgacggatgg
     4261 atcgtaaaca atgcgcaccg cttcggcatg gccagtatcg ccggagcaca cttcccgata
     4321 agtcggattt ggcgtatagc cgccggtata gcctgcggcg gtgctgtaaa cgccgggtaa
     4381 ctgccagaac agacgctcca caccccagaa acaacccatc gcaaaaatgg caatctccat
     4441 tccgtcaggt acattggtca ttgagtgacc gttgaccgca tgcagtgtgg ctacgggcat
     4501 cggggtgtta cgtccaggca gggcatcggc gggggaaacc agatgctttt tatcaaataa
     4561 actcatggtg tcgctctccc gaaaatcggt cattggggtt aaggttgtaa caagaggcgt
     4621 atttgcacac aataaccacc gtgaataggt ctaaagtaaa acataagaaa tattttgggt
     4681 ttagtctgct ttttaatcca tattactgga tttttgttaa gccgtttaac ggcgttccag
     4741 gggcaggaaa aaaggatatt caggagaaaa tgtgcgctat atccgacagt tatgctgtgt
     4801 aagcttactc tgcttaagcg gatctgccgt cgccgcgaac gtccgtctac aggtcgaggg
     4861 gttatcggga gagctggaaa agaacgttcg cgcgcagctt tctacgattg aaagtgatga
     4921 agtgacgcca gaccgtcgct ttcgcgcacg cgtcgatgat gccatccgcg aaggtctgaa
     4981 agcgctgggc tattaccagc cgaccattga atttgatctc cgtccaccgc caaagaaagg
     5041 gcggcaggta ttgatcgcca aagtcacgcc aggcgtgccg gtgttaattg gcggcaccga
     5101 tgtggtattg cgcggcggcg cgcggaccga taaagactat ttgaaattgc tcgatactcg
     5161 cccggctatt ggcacggtac tgaaccaggg tgattatgaa aatttcaaaa agtccttaac
     5221 cagcattgcg ttgcgtaaag gttatttcga tagcgaattt accaaagcac agctgggcat
     5281 tgcgctcggc ctgcataaag ccttctggga tattgattat aacagtggcg aacgttaccg
     5341 ctttgggcat gtgacctttg aaggatcaca aatccgcgat gaatacctgc aaaatctggt
     5401 gccgtttaaa gagggcgatg agtacgaatc gaaagatctg gcagaactga accgtcgact
     5461 ttctgctacc ggctggttta actcggtggt ggtggctcca caatttgata aagcgcgcga
     5521 aacgaaagta ttaccattga cgggcgtggt ttcgccgcga acagaaaaca ccatcgaaac
     5581 cggcgtcggt tactctacgg acgtgggacc gcgcgtgaaa gcgacgtgga aaaagccgtg
     5641 gatgaactct tacagtcaca gtttgaccac cagtaccagt atttccgcgc cggaacagat
     5701 cctcgacttc agctataaaa tgccgctgct gaagaatccg ctggaacaat attatttggt
     5761 gcagggcggt tttaagcgca ctgacctgaa cgataccgag tctgactcca ctacgctggt
     5821 ggcttctcgc tactgggatc tctccagcgg ctggcagcgt gccattaacc tgcgctggag
     5881 tttcgaccac tttacccagg gtgaaattac caacaccacg atgctgtttt atcctggggt
     5941 gatgattagc cgcacgcgtt ctcgtggtgg cctgatgcca acctggggcg actcgcaacg
     6001 ctactctatc gactactcca acacggcctg gggctcggat gttgatttct ccgttttcca
     6061 ggcgcagaac gtctggatcc gcacactgta cgatcgccat cgttttgtga cacgcggcac
     6121 gctgggctgg attgaaacgg gtgatttcga caaagtaccg ccggatctgc gtttcttcgc
     6181 cgggggcgat cgcagtattc gcggctacaa atacaaatct atcgccccga aatacgccaa
     6241 cggtgacctg aaaggggcct cgaagctgat aaccggatcg ctggaatacc agtacaacgt
     6301 gaccggaaaa tggtggggcg cggtgtttgt cgatagcggc gaagcggtaa gcgatattcg
     6361 ccgcagcgac tttaaaaccg gtaccggtgt cggcgtgcgc tgggaatcgc cggtcgggcc
     6421 aatcaaactc gattttgccg tgccggtcgc ggataaagac gaacacgggt tacagtttta
     6481 catcggtctg gggccagaat tatgagttta tggaaaaaaa tcagcctcgg cgtggttatc
     6541 gttatcttac tgttgctggg atcggtggcg tttctggtgg gcaccaccag cggcctgcat
     6601 ctggtattta aagcggcgga tcgctgggtg ccaggactgg atattggcaa ggtcaccggc
     6661 ggctggcgtg atctcacctt gtctgacgtt cgttatgagc agccaggcgt ggcggtaaaa
     6721 gcgggcaatc tgcatctggc ggtcgggctt gagtgcctgt ggaacagtag cgtttgtatt
     6781 aatgatctgg cgctgaaaga cattcaggtc aacatcgaca gtaaaaaaat gcctccttct
     6841 gaacaggttg aagaagagga agatagcggt ccgttggatc tctccacgcc gtatcccatt
     6901 accatgacac gggtggcgct ggacaacgtc aacatcaaga ttgatgacac cacggtatcg
     6961 gtgatggact tcacctccgg cctgaactgg caggagaaaa ccctgaccct gaaaccgaca
     7021 tcgctgaaag gcctgttgat tgctttgccg aaagtggcgg aagcggcaca ggaagaagtg
     7081 gtcgaaccga aaattgaaaa tccgcagccg gaggaaaagc cgctcggcga aacgctgaaa
     7141 gatctctttt ctcgcccggt attgccggaa atgaccgacg tgcatttgcc gcttaacctg
     7201 aacattgaag agtttaaggg cgagcagctg cgcgtgacgg gcgacacgga catcaccgtg
     7261 agcaccatgc tgctgaaagt gagcagcatt gacggcaata ctaaactgga cgccctggat
     7321 atcgactcca gtcaagggat cgtcaacgcc agcggcacgg cgcagctgtc agacaactgg
     7381 ccggtggata tcaccctcaa cagcacactg aacgtggagc cgttgaaagg tgaaaaggtg
     7441 aagctgaaag tgggcggtgc gctgcgcgaa cagctggaga taggcgttaa cctttccggt
     7501 ccggtggata tggatttacg cgcccagacg cgactggcgg aagccggatt gccgctcaac
     7561 gtggaagtga acagcaaaca gctttactgg ccgttcactg gtgagaagca gtatcaggcg
     7621 gatgatctga aactgaaact taccggcaaa atgaccgatt acacgctctc tatgcgcacg
     7681 gctgttaagg gccaggagat cccgccagcc accattaccc ttgatgccaa aggtaatgaa
     7741 cagcaggtca atctcgacaa acttaccgtc gcggcgctgg aagggaaaac ggaacttaag
     7801 gcgttgctcg actggcagca ggccattagt tggcgcggtg agctaacgct taacggcatt
     7861 aacaccgcca aagagatccc ggagtggccg tcgaaactca atggcttgat taaaacccgc
     7921 ggtagcctgt acggcggcac ctggcagatg gaggtgccag aactgaagct gaccggtaac
     7981 gtcaaacaga acaaagtgaa cgttgacggc acgctgaaag gcaacagtta tatgcagtgg
     8041 atgatcccag ggctgcatct ggaactgggg ccaaacagtg ccgaagtgaa aggcgagctg
     8101 ggggtaaaag atctcaatct tgatgccacc atcaacgcgc cggggctgga taacgcgctg
     8161 ccggggcttg gcggtacagc gaaagggctg gtgaaagtac gcggcacggt ggaagcgcca
     8221 caactactgg cagatatcac cgcgcgcggc ctgcgctggc aggaactttc cgtggcgcag
     8281 gttcgcgtgg aaggcgacat taaatccacc gatcagatcg ccgggaaact cgacgtacgc
     8341 gttgagcaaa tttcgcaacc ggatgtaaat atcaacctcg tcaccctgaa tgccaaaggc
     8401 agtgaaaagc agcacgagct acagttgcgg attcagggcg agcctgtctc cgggcagctt
     8461 aatctggcag gaagttttga tcgcaaagaa gaacgctgga agggaactct tagcaatacc
     8521 cgcttccaga cgccggtcgg cccgtggtcg ctgacccgcg atattgcgct ggattaccgc
     8581 aataaggagc aaaaaatcag catcgggcca cactgctggc ttaacccgaa tgcggaactg
     8641 tgcgtgccgc aaactatcga tgcgggtgcc gaagggcgtg cggtggtgaa tctcaaccgc
     8701 ttcgacctgg ccatgctgaa accatttatg ccagaaacca ctcaggccag cggtctcttc
     8761 acgggtaaag cggacgttgc ctgggacacc acgaaagagg ggctgccgca gggcagtatc
     8821 accctttcgg ggcgtaatgt gcaggtgacg caaaccgtca acgatgcggc gctgcccgtg
     8881 gcgtttcaga cgctgaatct gacggcggag ttgcgtaaca accgtgccga attgggctgg
     8941 accatccgcc tgaccaataa cggccagttt gatggtcagg tgcaggtgac cgatccgcaa
     9001 ggccgccgta atcttggtgg caacgtcaat atccgtaact tcaaccttgc gatgataaac
     9061 cccatcttta cccgtgggga aaaagcagcg gggatggtga gtgccaactt gcgtctgggt
     9121 ggtgatgtgc aaagtccgca gttgtttggt cagcttcagg ttacgggggt ggatatcgac
     9181 ggcaacttta tgccgtttga tatgcaaccg agccagcttg cggtcaactt taacggtatg
     9241 cgctcgacgc ttgcaggtac cgtgcggacc cagcagggtg agatctacct gaacggcgac
     9301 gccgactgga gccagattga aaactggcgg gcgcgagtaa cagcgaaggg cagtaaagtg
     9361 cggatcaccg tgccgccgat ggtacgaatg gatgtatcac cagatgttgt attcgaggct
     9421 acaccaaacc tgtttaccct tgatggtcgc gtggatgtcc cgtgggcgcg cattgtggtg
     9481 catgagctgc cggaaagcgc ggtgggcgtc tccagcgatg tggtgatgct taacgataac
     9541 ctgcaaccgg aagaggcaaa aacggcgtcg attccgatta acagcaacct gattgtccac
     9601 gttggcaaca atgtgcgcat tgacgccttt ggcctgaaag cgcggctgac gggcgatctc
     9661 aacgtcgttc aggacaaaca agggctgggc ctgaacgggc agatcaacat ccctgaaggg
     9721 cgcttccatg cctatggtca ggatctgatt gtgcgtaagg gtgagctact gttctctggt
     9781 ccgccagatc aaccgtatct taatatcgaa gctattcgta acccggatgc tacagaagac
     9841 gacgtcatcg ccggagttcg cgtcactggt ctggcggacg aaccgaaagc ggagatcttc
     9901 tctgacccgg cgatgtcgca acaagctgca ttgtcttatt tgctacgtgg acaagggctg
     9961 gagagcgatc agagcgacag cgcggcgatg acctcgatgc tgattggcct gggggttgcg
    10021 caaagtggcc agattgtggg taaaatcggc gagacgtttg gcgtaagcaa tttagcgctc
    10081 gacaccgagg gagttggcga ctcctcccag gtggtggtca gcggctatgt attgccaggc
    10141 ctgcaagtga aatacggcgt gggtatattt gactctatag caacactcac gttacgttat
    10201 cgcctgatgc ctaagctata tctggaagcc gtgtctggtg tagaccaggc actggatttg
    10261 ctctatcagt tcgagtttta gcaatgcgaa tatttgtcta cggcagttta cgccacaaac
    10321 aaggcaacag tcactggatg accaatgccc agttactggg cgatttcagt atcgataact
    10381 accagttgta tagcctgggc cactatccag gcgcagttcc ggggaacgga acagtacacg
    10441 gtgaagttta tcgtattgat aacgccacgc tggccgaact tgatgccttg cgcaccaggg
    10501 gcggtgaata cgcgcgccag ttgattcaga cgccgtacgg gagtgcatgg atgtacgttt
    10561 atcaacgacc cgtcgatgga ttaaagctaa ttgaaagcgg cgactggtta gacagggata
    10621 agtaataata aaagcacgcc accttcgggt ggcgttgttt tatgcgatgt atctcgcgat
    10681 tctttttgta actttcccat cttttgcttt tctctccggg ccgctttcca tatctgcaat
    10741 cgcataaaaa actctgctgg cgttaacaaa tgtacagggg taaaacgggg acacgctttg
    10801 aacggtgagt atgagtgagg ccggctaact tccagcaagt ggctatgaac agcaaaatgg
    10861 tggtcgagtt tagcttgttc ttaccgttca agcctttcaa ctgctgaaaa taataatagt
    10921 ggagtagaga gtttataaat tgcgggcata aaaaaaccct tactaaccga agcccggtgt
    10981 tcagggttat tgcgccagaa gaacttattt attcttggcg cgctcgaagg aagcaacgat
    11041 ttcagcttta gcggcttctg cgttttccca accttcaact ttaacccact tgcctttttc
    11101 gaggtctttg tagtgctcga agaagtgagc gatttgcgct ttcagcagct ctggcagatc
    11161 gttaacgtct ttaatgtgat cgtattcttt gctcagcttg gtgtgcggaa cagcaatcag
    11221 tttcgcatct tcaccggctt cgtcggtcat tttcagtacg ccaaccggac ggcagcggat
    11281 cacagaaccc ggctgcagcg ggtacggagt cgggaccagt acgtcaaccg ggtcaccgtc
    11341 cagagacaga gtgtggttga tgtaaccgta gttgcacgga tagaacatcg cggtggacat
    11401 gaagcggtca acgaacagtg cgccgctctc tttgtcgatt tcgtatttga ttggatctgc
    11461 gttagccggg atctcaataa caacgtagat gtcttccggc agatctttac ccgcagggac
    11521 gttgagtaag ctcatgtctg tttcctttaa aaatatgtgg caaacaagtg ccgagtatta
    11581 tagccaactc gcgccgaatg tcttcgcttg ttttcgtctt cattttcctt tttcgccagt
    11641 tttaagacgg tatacagagc aggaaaatcc ataaccgtag ccgcattttt catagtgaga
    11701 tgaaagcgat tacaaacttg tgattaacgt tttatttact tttttgaagt gtgatgtaac
    11761 gcaatccgtt acataacgaa ttgtctatag ttttttcgcg aacatctttt caccaataat
    11821 aattaccccg acgaggacaa ccctatgtgg aaacgcttac ttgtagtctc tgcagtctcg
    11881 gcagccatgt cgtctatggc gttggccgct ccattaaccg taggattttc gcaggtcgga
    11941 tcggaatccg gctggcgcgc cgcagaaacc aatgtggcga aaagtgaagc cgaaaaacgc
    12001 ggtatcacac tgaaaattgc cgatggtcag caaaagcagg aaaaccagat taaagcggta
    12061 cgttccttcg tcgcacaagg ggtggatgcg atctttatcg ctccggtggt ggcgacgggt
    12121 tgggaaccgg tattaaaaga ggcgaaagat gccgaaatcc cggtcttctt gcttgaccgt
    12181 tccatcgatg tgaaagacaa atctctctat atgaccactg tcaccgccga caacatcctc
    12241 gaaggcaagt tgattggtga ctggctggta aaagaagtga atggcaaacc atgcaacgtg
    12301 gtggagctgc agggcaccgt tggggccagc gtcgccattg accgtaagaa aggctttgcc
    12361 gaagccatta agaatgcgcc aaatatcaaa attatccgct cgcagtcagg tgacttcacc
    12421 cgcagcaaag gcaaagaagt gatggagagc tttatcaaag cggaaaacaa cggcaaaaac
    12481 atctgcatgg tttacgccca taacgatgac atggtgattg gtgcaattca ggcaattaaa
    12541 gaagcgggcc tgaaaccggg caaagatatc ctgacaggct ctatcgacgg cgtgccggac
    12601 atctacaaag cgatgattga tggcgaagcg aacgccagcg ttgaactgac gccgaacatg
    12661 gcaggccccg cttttgatgc gctggagaaa tataaaaaag acggcaccat gcctgaaaag
    12721 ctaaccctga ccaaatccat cctttatctg cctgataccg caaaagaaga gttagagaag
    12781 aagaaaaata tggggtattg agggttgcta tgcctgatgc cgattcgtag gccggataag
    12841 gcgctcgcgc cgcatccggc ggtggtgcgc tgaagcctga tgcgacgctt accgcgtctt
    12901 atcatgccta ctgggagcac gctttacacc gggggaaacc atgacgaccg accaacacca
    12961 ggaaatcctc cgcaccgaag gattaagtaa atttttcccc ggtgtcaaag cgttagacaa
    13021 cgttgatttc agcctgcgcc gtggtgaaat catggcgctg ctcggtgaaa acggggcggg
    13081 aaaatcgacg ctcatcaaag cattaaccgg tgtataccac gccgatcgcg gcactatctg
    13141 gctggaaggc caggctatct caccgaaaaa caccgcccac gcacaacaac tcggcatcgg
    13201 caccgtctat caggaagtca acctgctacc caatatgtcg gtcgctgata atctattcat
    13261 aggtcgcgaa cccaaacgtt tcggccttct acgccgtaaa gagatagaaa agcgcgccac
    13321 cgaactgatg gcatcttacg gtttctccct cgacgtgcgc gaaccgctca accgcttttc
    13381 agtcgcgatg cagcaaatcg tcgccatttg ccgggctatc gatctctccg ccaaagtgct
    13441 gatcctcgat gaacccaccg ccagtctcga cactcaggaa gtagagttac tgtttggcct
    13501 gatgcgtcag cttcgcgatc gcggcgtcag tctgatcttc gtcactcact ttctcgatca
    13561 ggtctatcag gtcagcgatc ggatcaccgt cttacgtaac ggcagttttg tgggctgtcg
    13621 ggaaacccgc gagctaccgc agatcgaact ggtaaaaatg atgctggggc gtgaactgga
    13681 cacccacgcg cttcagcgtg ccgggagaac attgctgagc gacaaacctg ttgccgcgtt
    13741 caaaaattac ggcaaaaaag gaacgatcgc gccgtttgat ctcgaagtac gccccggcga
    13801 gatcgtcggt ctggcaggct tgctgggatc aggacgtacc gaaaccgccg aagtgatctt
    13861 cggtatcaaa cctgctgata gcggcacggc gttgatcaaa ggcaaactgc aaacgctacg
    13921 ctcgccacat caggcttcgg tactgggcat tggcttctgc ccggaagaca ggaaaaccga
    13981 tggcattatc gccgccgcct cggtgcggga aaatatcatc ctcgctctcc aggcccagcg
    14041 cggctggcta cgacctattt cccgtaaaga acagcaagag attgccgaac gctttatccg
    14101 ccagcttggc attcgcacac cttccaccga acaaccgatt gaatttctct ccggcggcaa
    14161 tcagcaaaaa gtgttgcttt cacgttggct actgacccga ccgcaatttc tgattctcga
    14221 cgagccaacg cgcggaattg atgttggtgc ccacgccgag atcatccgcc tgattgaaac
    14281 gctgtgcgcc gatgggctgg cgctgctggt gatctcctcc gagctggaag agctggtggg
    14341 ctatgccgat cgggtgatta tcatgcgcga tcgcaaacag gtggcggaga tcccgctggc
    14401 agagctttcc gttccggcga tcatgaacgc cattgcggcg taaggagaac agtgtgatgc
    14461 ctcaatctct cccggacact acgccgccga aaaggcgttt tcgctggcca acaggaatgc
    14521 cgcagctggc ggcactgttg ctggtgctgc tggttgatag cctggtggcc ccacatttct
    14581 ggcaggtggt gcttcaggat gggcgtttgt tcggtagccc tatcgacatt cttaaccgtg
    14641 cggcccccgt tgcgctactg gcgattggca tgacgctggt tatcgccacc ggtgggattg
    14701 atctctccgt aggggcggtg atggctatcg ccggagccac aacggctgcg atgacggtcg
    14761 cgggattcag cctgccaatt gttttgttaa gcgccctggg cactggcatc ctggcaggat
    14821 tgtggaacgg catactggta gcgatcctca aaattcagcc gtttgtcgcc accctgatcc
    14881 tgatggtcgc cgggcgcggc gtggcgcaac tgatcacctc cggacagatc gtcacattta
    14941 actcaccgga tctctcctgg ttcggcagtg gatcgctgtt gttcctgcca acgccagtca
    15001 ttattgcggt gctgacgctt ctcctgttct ggctgttgac ccgcaaaacg gcactgggga
    15061 tgtttatcga agccgttggt atcaacattc gggcggcaaa aaatgccggg gtaaacacgc
    15121 ggatcatcgt catgctcacc tacgtgttga gcgggctatg tgcggcgatt gcgggcatta
    15181 tcgtggcggc ggatattcgc ggtgccgatg ccaacaatgc cggattatgg ctggagctgg
    15241 acgccattct tgcagtggtg attggcggcg gatcgctgat gggcgggcgc tttaacctgc
    15301 tgctttcggt ggtgggggcg ctgattattc agggaatgaa caccggaatt ttgctttctg
    15361 gctttccgcc ggagatgaac caggtggtga aagcggtggt ggtgctttgc gtgctgattg
    15421 tccagtcgca acgctttatc agtctgatta aaggggtacg taaccgtgat aaaacgtaat
    15481 ttgccactga tgatcaccat cggcgtcttt gtgttgggtt atctctactg tctgacccag
    15541 tttcccggtt ttgcttccac aagagtgatc tgcaatatcc tgactgataa tgcctttctt
    15601 gggataatcg ccgttggtat gacctttgtg atcctctccg gtgggatcga tctctccgtc
    15661 ggttcggtga tcgcctttac cggcgtgttt ctggcaaaag tgattggcga tttcggcctc
    15721 tcgccgctgc tggcgtttcc actggtgctg gtgatgggct gtgccttcgg cgcatttatg
    15781 gggcttttga tcgacgccct gaagatcccg gcatttatca ttaccctggc ggggatgttc
    15841 tttttgcgcg gcgtcagcta tctcgtttcg gaagagtcga tcccgataaa ccatcctgtt
    15901 tatgacacgc tctcaagcct tgcgtggaaa atccctggcg gcggtcgctt aagtgcgatg
    15961 gggctgctga tgctggcggt ggtggttatc ggcatattcc tcgcgcatcg tacgcgtttt
    16021 ggtaatcagg tgtacgccat tggcggcaac gcaacgtcgg cgaacctgat ggggatttcc
    16081 actcgcagca ccactattcg catttatatg ctctctaccg ggctggcgac actggcgggg
    16141 attgtcttct cgatttatac ccaggccgga tatgcgctgg cgggcgtagg tgtggaactg
    16201 gatgctatcg cctcagtggt aattggcggt acgcttttga gtggtggcgt tggaacggta
    16261 ttagggacgc tttttggcgt ggcgattcag ggactgattc agacttacat aaactttgat
    16321 ggcacgctga gctcatggtg gacgaaaatc gccatcggta ttttgttgtt tatttttata
    16381 gcattacagc gtggattaac ggtgctgtgg gagaatcgtc agagttcgcc tgtgacaaga
    16441 gtcaacattg cgcagcgata aaaacgcctc tccgtgtgga gaggcgcagg agattacgcg
    16501 tccgggaact cacggataaa gcgttcgaca tcttcaacca tatggtcgtt gccgacaaag
    16561 aatgaacggc gctggtgcag ggtttccggg atgatatcca gaatacgctc tttgccatcg
    16621 ctcgctttac cgcctgcttg ttccgccagg aatgccatcg ggttgcactc atacagtaaa
    16681 cgcagtttac cgtccgggtg gctggcggtg cttgggtaga gataaatacc gcctttcagc
    16741 aggttacggt ggaaatccgc gaccagtgaa ccgatataac gtgaggtata cgggcggttg
    16801 gtggatttat cttcttcctg gcagaattta atgtacttct tcaccccgtt cggaaactta
    16861 atgtagtttc cttcgttgat ggagtaggtt ttgcctttct ccgggaagcg catccgttcc
    16921 tggcacaggc agaaaacacc cagcgaagga tcataagtaa aggcgtgaac accgcatccg
    16981 gtggtgtaaa ccagcatagt agaggagccg tataccacgt aacctgccgc aacctgtttg
    17041 ttaccaggct ggaggaaatc ttcttccgtt accggcgtgc caacaggcgt aacgcggcgg
    17101 tagatggaga aaatggtacc gacagagacg ttaacatcga tgttggacga gccatccagt
    17161 gggtccatca gcaccacgta ttttgcgtgt tcacagcctt caaagacgac aatctcatct
    17221 tcttcttcag aggcaatgcc cgcaacgata tcgcgtgctt tcagtgcggc tttcagtttc
    17281 tcattagcga acaggtcgag tttctgctga acctcgccct gcacgttctc agcaccgctg
    17341 gcacccagga tatcaaccag tcctgctttg ttgatatcgc gatggataat cttggcgccc
    17401 agttttattg ccgacagcaa agcagtgagc tcaccggtag catgagaaaa ctcgtgctgc
    17461 ttttcgacaa taaattcacc taacgttttc ataaaacttt ccctgcaatg tttatggagt
    17521 aaagcgaccg caacaatctt aacaaataat cccaatgttg cgctcaggtg aatcgcgcca
    17581 gcaaattacg gattatcctg aaatgcgttt ctcacttgcc cgacatatgc gtaaaatgag
    17641 cgccagatta aaaaaggata gtgacgtatg cgcattcata ttttaggaat ttgtggcacg
    17701 tttatgggcg gtctggcgat gctggcgcgc cagttaggcc atgaagtaac gggttcggac
    17761 gccaatgtgt atccgccgat gagcacctta cttgagaagc aaggcattga actgattcag
    17821 ggttacgatg ccagccagct cgatccacag ccggatctgg tgattattgg taacgccatg
    17881 acccgtggaa atccgtgtgt ggaagcggta ctggaaaaaa acatccctta tatgtcaggt
    17941 ccgcagtggc tgcacgattt tgtgctgcgc gaccgctggg tgctggccgt tgccggtaca
    18001 catggcaaaa ccaccaccgc gggaatggca acctggattc tggaacagtg cggttacaaa
    18061 ccgggatttg tgatcggcgg tgtgccgggg aactttgagg tttcggcgcg tctgggcgaa
    18121 agcgacttct ttgttatcga agcggatgag tatgactgcg ccttcttcga caaacgctct
    18181 aaatttgtcc attactgccc gcgtacgctg atcctcaaca accttgagtt cgatcacgcc
    18241 gatatctttg acgacctgaa agcgatccag aaacagttcc accatctggt gcgtatcgtt
    18301 ccggggcagg gccgtattat ctggccggaa aatgacatca acctgaaaca gaccatggcg
    18361 atgggctgct ggagcgagca ggagctggtg ggcgagcaag gtcactggca ggcgaaaaag
    18421 ctgaccaccg atgcttccga atgggaagtc ttgctggatg gcgaaaaagt gggcgaagtg
    18481 aaatggtcgc tggtaggcga acataatatg cacaatggcc tgatggcgat tgcggcggct
    18541 cgccatgttg gtgtagcgcc ggcagatgcc gctaacgcgc tgggttcgtt tattaatgct
    18601 cgtcgccgtc tggagttgcg tggtgaagcg aatggtgtga cggtatatga cgattttgcc
    18661 catcacccga cggcgattct ggcaacgctt gcggcgctgc gtggcaaagt tggtggtacg
    18721 gcgcgcatta ttgccgtgct ggaaccgcgc tcaaacacca tgaaaatggg gatctgcaaa
    18781 gacgatctgg caccttcatt aggtcgtgcc gatgaagtct tcctgctgca accggcgcat
    18841 attccgtggc aggtggcaga agtggcagaa gcctgcgttc agcctgcaca ctggagcggc
    18901 gatgtggata cgctggcaga tatggtggtg aaaaccgctc agccgggcga tcacattctg
    18961 gtgatgagta acggcggttt tggtgggatc catcagaaac tgctggatgg gttagcgaag
    19021 aaagcggaag ccgcgcagta attcacagcc tcagcctgat agagtgtgtg taggccggat
    19081 aaggcgttca cgccgcatcc ggcatttgag catagtgcct gatgcgacgc ttgatgcgtc
    19141 ttatcaggcc tacaggtgca ccgcatccgg caaaccatac tcacatcaac aacgaaaatt
    19201 acccttcgtt ttccgctaac tcacgcagat actggaaaat ctggcgcgcg gatttcggcg
    19261 gcttattccc ttctttctct ttcttcgcgt tacggatcag agtacgcagt tgctgacgat
    19321 cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttgc tcgatcagac
    19381 gatcgcgcag gttttccagt ttatggaaca acaccacttg ctggttgtgg cggtttttca
    19441 gcttatccag cgcctgacga ataggctcaa catcgcgctg gcgcagcatc ttaccgatga
    19501 gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca atagccgcgc
    19561 gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca acaatttccg
    19621 cgccaaggcg ttttagctcc tcggcatcgc gtttaatttc acttttactg acccagataa
    19681 tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag tcttcgggct
    19741 gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa gatgcgcact
    19801 gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac ttgcaatgaa
    19861 agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt cgactgcgct
    19921 ggagttggcc tcaggcaaat cggacggtgc agaagttgcc gtcagcaaga ccaccggcat
    19981 tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagcg atggcgcgtt
    20041 ggggatcact gtttatcacc agaaccgcaa aggtagcgca tcgtctaccg atttaagccc
    20101 gcaggccatt gcccgcaccg tacaggcggc gctagatatt gcccgttata cctcgccaga
    20161 tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccgg atctcgactt
    20221 gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc gcgcagaaca
    20281 ggcagcattg caggcggaca aacgcatcac caataccgaa ggtggcagct ttaacagcca
    20341 ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact gctctacgcg
    20401 tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc gtgattacgc
    20461 ctacaccatt ggtcgtgcga tgagcgatct gcaaacgcca gagtgggtcg gggccgactg
    20521 tgctcgccgc actttatcgc gtctgtcacc gcgtaaactc tccaccatga aagcgccggt
    20581 catttttgcc aatgaagtgg caaccgggct ttttgggcat ctggtggggg cgatagcggg
    20641 tggatcggtt taccgtaaat ctaccttcct gctggattcg ctgggtaaac aaattctgcc
    20701 ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcct ctacgccatt
    20761 cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca tcctgagtca
    20821 gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg gacatgcggg
    20881 cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa tgctcaaaga
    20941 gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg ccattaccgg
    21001 tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc agtatccggt
    21061 tagcgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg tcaccgtcgg
    21121 taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc cggagatgaa
    21181 aatcgccggg cagtaaaaaa ctggcgcgac ctgccgcgct ataaactaaa aaattacaca
    21241 aaatcattcg cactgcatcg agacggaaag tgaatgagga ccggggagcg tactcgcagt
    21301 acgtgactcg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat gaagtgtaaa
    21361 taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt ctcctcgttg
    21421 gtattcagtt cggcgtcgtt tgccgctgat cttgaagaca atatggaaac cctcaacgac
    21481 aatttaaaag tggtcgaaaa agccgataac gcggcgcaag tcaaagacgc gttaacgaag
    21541 atgcgcgccg cagcgctgga tgcgcaaaaa gcgacgccgc cgaagctcga aggcaaatca
    21601 ccggacagcc cggaaatgaa agacttccgc cacggtttcg atattctggt cggtcagatt
    21661 gacgacgcgc tgaagctggc aaatgaaggt aaagtaaaag aagcgcaggc tgccgcagag
    21721 caactgaaaa cgacccgcaa cgcctatcac cagaagtatc gttaattcct tatttccctg
    21781 ttgcctgcac tcaggtaaca gggaacttat catcgcaaat gatgcaccac ctgattgctg
    21841 ctgccgcgcc agatcaggga agggtctttt aaatcctgca caaatttgcc gtcgaccagc
    21901 acgttaatca aatcaacaac ctgcatttgc gcagcgttga gttcgtcgag tttatagcct
    21961 gtccacaccc agatgtcttt acccggacat tcggcgcgga tgcgttgtac cagtttcaga
    22021 atatccggca cgttttgcgg atgcagcgga tcgccgccgg agagggaaat accctggcgt
    22081 ttgatgcgag tgtcattcag atcgttaatg atctggtctt ccattgcttt ggtaaatggc
    22141 tgaccggaat ttacccgcca ggtgcttttg ttatagcaac cggggcattc atgaacacac
    22201 ccggagacaa acagggtgca gcgagtgccg gggccgttga cgatgtcgac aggatagtac
    22261 tgatgataat tcattatttc gcctggctta tcagtccgcg tgtaggctgc atccagtaat
    22321 caacgcctga tgcgacgctg tcgcgtctta tcaggcctgg agccgttgcc aaatgtaggc
    22381 cggataaggc gtttacgccg catccggcat ttgcttaacg cagagtgaag attaacctat
    22441 ctgcccattc cccaaatgtt taacgcggcg cttaacttct tcctgcttac cggcgttaaa
    22501 tggacgtgca tccgggctac ctaaatatcc gcacacgcgg cgagtcaccg acacacggga
    22561 ggcgtcatgg ttaccacatt tcggacaagt gaagcctttg ctggtgcact cgaactcacc
    22621 ggtaaagcca cactcgtagc actcatcgat cggcgtattg gtgccgtaat acggtacatg
    22681 ctgatagctg taatcccaga catcttccag cgccttcagg ttgtgctgaa tgtttggata
    22741 ctcgccgtag caaatgaaac caccgttcgc cagcggcggg taaggcgctt caaagtcgat
    22801 cttgtcatac gggttcacct ttttctccac atcaaggtgg aaactgttgg tgtagtaacc
    22861 tttatcggtc acgcccggca ccacgccaaa ctcagcggta tcgagacggc agaagcggtc
    22921 gcacaggttc tcactcggcg tactgtagag gctgaaaccg tagccagttt cttctttcca
    22981 ctgatccacc gcctgacgca gacgctcaac aatcgcaata cctttcgcgc gcagctgctc
    23041 gttgtcgtaa acatgctcgc cgccgaacag cgcgttaatg gtttcgtgga tgccgatgta
    23101 acccagcgaa atagatgcac gaccgttttt gaagatttca gaaacgtcgt cgtcagcatt
    23161 cagacgcacg ccacaagcgc cttccatata gaggatcggg gctacgcgcg ctttcacgcc
    23221 ttcgagacga gcgatacggg tcatcagtgc cttacgtgcc agcaccagac gttcatccag
    23281 cagcttccag aaggtggctt catcgccttt tgcttccaga gcaatacgcg gcaggttcag
    23341 gctgatcacg ccgaggttgt tacgaccatc gtggatctgc tcgccgtttt cattttccca
    23401 cacgccgagg aagctgcggc agcccatcgg ggttttaaac gaaccggtga ctttcactac
    23461 ctgatcgtag ttcaggatat ccggatacat gcgcttgctt gcgcactcca gcgccagctg
    23521 tttgatgtcg tagttcggat cgcctttttt atggttcagg ccatcgcgaa tcgcaaacac
    23581 cagtttcggg aacaccgccg ttttacggtt tttaccgagg ccggcgatac ggttacgcag
    23641 gatagattcc tgaatcaggc gcgactccca gctggtgccc agaccaaaac caaaggttac
    23701 aaacggcgtt tgaccgttgg cggtgtgcag ggtgtttact tcgtactcca gcgactggaa
    23761 ggcatcgtag cactctttga tggtacgaga gttagcgtag ccttcggcgt ccgggatgtt
    23821 ccactcttct gcggtcttgc gatgtttgtt gtagctggcg gtgacaaacg gtgccagcac
    23881 ttcatcgata cggttaatgg tggtgccgcc ataaatatgg ctggcgacct gggcaataat
    23941 ctgcgcagtt accgcggttg ccgtagagat cgacttcggc ggttcaatct cggcgttccc
    24001 cattttaaag ccctgggtca gcatgccttt caggtcgatc aacatgcagt tgaacatcgg
    24061 gaagaacggt gagtaatcga gatcgtgata gtgaatatca ccacgttcat gtgcctgcac
    24121 cacgtcacgc ggcagcaggt gctggcgtgc atagtgttta gccacaatcc cggccagcag
    24181 gtcgcgctgg gttggaatca ccttgctgtc tttgttggcg ttttcgttga gcaacgaggc
    24241 gttggtctgc tcaaccagac cacggatctc ctgattcagg cgaccgcgtt tttcacgttc
    24301 aatgtcgcga tcgtgacggt actcgatgta agcacgagcc agttgtttgt atggacccga
    24361 cataagctgg ttttcaacgg cggtctggat ctcgttgata tccacctggc tgcggccctg
    24421 catctgctcg ctgacaaccg cggcaacagt ggcgcaataa tctgcatcat cgacttccgc
    24481 tgctttagct gcacgcagaa tcgcttcttt gatgcgctct gatttaaacg gcactttgca
    24541 gccgtctcgt ttcatcacat gcggtgtcat gatcgctcca tatttttaag aacaggttat
    24601 ccacagaaat tgggaaaggt gttttccggt tttttcgttt cgttttccgg cgctttccgc
    24661 aatcgccatc cgcttatcca caacgccgca cctttttcgt ggggagttgt agtagcaatt
    24721 atagtcgatt aatacaacat attgggtcgg gacgcatttt aaagtctata tatagtgctt
    24781 tgcatcaagg atgtttgaac tttttttgat gtagctcaaa gtaaaaagca gagcgtacgg
    24841 atgacgggcg ctacagcgtt atgtaaattt tttaatgaat tttttgatta taaaatcaac
    24901 atgctgaaag gcaaaacccc ggaatgacca gtattaccgg ggcttagcgg atacttactt
    24961 ctgtaaccac cagacagcct caaaaggccg taaagtcatg gcacagggtt gtggtgaggc
    25021 ttcttcgtag ttatgcatca caagctgcca gttgccgagc attttccctg gctgccaggg
    25081 ttgggtctca cggctaaggt tggcaatgac cagtaaggtt tgccccttcc actcacggcg
    25141 atagcaccac aatacagggc tgtttggcag cagatcctgg taatcgcccc atgtcaggac
    25201 ggcttcttgc ttacgcagtg cgattaactt ttggtaggtg taaaacaccg aggactcatc
    25261 ggccagcgct gcttctacgt tgatttcctg atagttatcg cccaggccaa tccacggttc
    25321 gccagccgta aacccggcat tatcgccgtt gctccattgc atgggtgtgc gactgttgtc
    25381 acgggattta ctggcgagga ttgccaataa ctcgtcggca tcacgaccat cgttgcgcag
    25441 ctcggcaaac atattgaggc tctccacgtc gcgatagtca gtaatgcgcg tgaaatgcgg
    25501 gttggtcatg ccaatctctt cgccctggta gatatacggc gttccctgca tgccatgcag
    25561 caccatcgcc agcatttttg ccgcaggcac gcggtattca ccttcatcac caaagcgaga
    25621 aacaatgcgc ggctgatcgt ggttacacca gaacaaggca ttccatgcca cgttgtgcat
    25681 tccttgttgc cagtggcgga acaatgtttt caacgccaca aagtcaggtt tagccagcgt
    25741 ccatttttca ccaccgggat aatcgacctt caggtgatga aaattaaagg tcatcgacaa
    25801 ttcactgcct gtcagtgccg cgtatcgctg gcaatgctca aggctggtgg aggacatttc
    25861 acctacggtc attaacccgc gtggcgtaaa cacatcgcgg ttcatctcgt gcaaaaactc
    25921 gtgtgctcgt ggcccgtcgg tgtagaagcg acgcccgtcg ccatccaggt cttcagggaa
    25981 acgcgggtct ttggagatca gattcaccac atccaggcgc aacccgtcga ccccacgatc
    26041 ggcccagaac tcacagactt ttttcagctc tgcgcgtacc gctggattct cccagttgag
    26101 atccgcctgt tctggtgcaa agagatgcaa atagtactgt tcgctttccg catgccagcg
    26161 ccacgcacta ccgccaaatt ttgaacgcca gttgttcggt ggcgtttctg gttcgccatc
    26221 gcgccagata taaaactggc ggtaagggct ttctttgttc agcgcctcgc gaaaccaggc
    26281 atgttgggta gaggtatggt taaacaccat atcgagaatg atacgaatcc cgcgcgattt
    26341 tgcctgcgtc accagttcgt caaaatcgtc cagcgtgccg taggtgggat caatcgccgt
    26401 atagttcgct acatcgtaac cgttatcgac ctgcggagag acataaaagg gggttagcca
    26461 gatggcatca acgcccagtt tatgcagata gtccaggcgt tggataacgc cacgtaaatc
    26521 gccggtgccg ctacccgtgg tgtcctgaaa actctttgga taaatctggt agataacgcc
    26581 gttttgccac cagtggggaa gattagtcat tacgttattc ctgcaaatgc gagggggcgc
    26641 aattgcgccc cgaagaaaat taaacaatgt ccagcgtgcc caggcggtat ttccgctgat
    26701 agataaacga ggtgagtaca atcgggatga tgatggcgat agccattgcc agcgcaaaca
    26761 cctgccagta gctcggttga atcgagagaa ttcccggcag gccgcctacg ccgatgccat
    26821 tcgccataac gccgttcagg ccgcatagca atcctgccag accagaacca atcatcgcgc
    26881 acagcatcgg gaagcgatat ttcaggttga tgccgtacat tgcaggctca gtgaccccaa
    26941 gccaggcgga gatagcggca ggcacggaga tctcgcgttc attgtgcttg cggctggaaa
    27001 tgataatgcc tatcacggcg gagccctgag cgatattcga cagcgcaatc agcggccaca
    27061 ctggcgtgcc acccatgctt tgaatcatct gcaaatcaat agcaagggtg gtctggtgta
    27121 cgccggtgat caccagcggc gcgtacagga agccaaacaa tgccgcacca atcggagcaa
    27181 agctgccggt catcaggtga cgtaccgcaa aggcaacgcc atcgccaatc atgcgaccaa
    27241 acggaccaat cagcgcatgg gcgaggaaca ccgcgaggat cagcgaacag acgggcacca
    27301 ccaccagata gaggtaatcc ggtacgatgc gtttaaggcg agtttcaata acgcccagcg
    27361 ccagcccggc taacagtgcc gggatcacct gcgcctgata gcccactttg gcgatgctga
    27421 acatgccaaa gtcccacact tccggcagct gctgcccgag cagataagcg ttcatcagct
    27481 ggggggaaac cagtgtcacg ccaagcacga taccaaggat cggcgtgccg cccatttttt
    27541 tcaccgctga ccagcaaatc ccgaccggca ggtagaagaa gatcgcttca ccgatcaacc
    27601 acagaaaatc gtagatcgtt tgcagggaag ggtacatttg cgccagcgtt tgaccgttgc
    27661 tcatgggcaa atcgccgatc acattgcgaa aaccgaggat caaaccgccg ctaatcaacg
    27721 cgggcagcaa cgggaagaag atctccgcga aatgagagat caactgctca tgccatttca
    27781 tattctgccg ggcggctttt tttacctgct ctttatcaac ctgcgcctgt ccggttgacg
    27841 ctattagtgc ttgatagtaa tcacccacgt tggtgccaat caccacctga aattgcccgg
    27901 cattggtgaa acagcctttc accatgggga gttgctcaat ttctttcggt ctggcattgg
    27961 ccggttggtt gaggacaaag tgcaggcgag taatacagtg gctcaccgtc gcaatattgc
    28021 cgcgcccgcc gaccagttca atcaaccgat cgatatccgt ttggtttatt ttgctcatca
    28081 taaagcccca tggcagatga catttttggt tggctgcaga atatattgcg cggaaaaatt
    28141 taaaaacggg aacgttcccg aaacgcagcg aagatcacaa tttatcgttc aggaaacgat
    28201 caggacaggg tggcggggat gatgatttgt tgcggttcgc tgcgcccggt tacctgtgcg
    28261 atcaattggc aggccgcctg gcgtccagct tcggcgtaac caggatctac ggtgacgatc
    28321 tccggatgga ggaatttcat taatggcgta ttaccgacgc tcgccagttg caaggtgtcg
    28381 atgcgttgct cttgcaggta tttacttgcg ccaagtgcca gcgtgtcggt tgcgcacagt
    28441 aaggcggtag tttcaggcgt aatcactttt gctacgttct catagccttg cttcatagca
    28501 aggcctggca gagcggcaac gggatgcagt ttatgcgctt tgcagaacgc caggtaggct
    28561 tcgtgacgtc gcttcccggt cgtcacgtca ctatgcggca cgccgagata actgatatta
    28621 cgatgcccct ggtcatacag ccgttgcatc aggatcttga ttgccccttc gtcgtcataa
    28681 cagaccgaag caaagccttt tgcgtcacgc gccagcagaa ccagcgatga ctgccagtgg
    28741 gctaacattt cttctgttat gccggtaaaa ccgaacagca ctacgccgtc gatattacgc
    28801 cgtttcagca cccccaaatg ttcggcaact aattgcgggg aaaactgact ttccatcatg
    28861 attgggtcgt aaccttgttc atagaacgct ggcagcatgg tttgaacggc gagattttct
    28921 gacaacgaat ccagacgggt aacaatgatg gcgaccactt tatcgctttg accacgcata
    28981 gcgcgcgcag agcgggaagg ggaaaatcca tgctgattca tcactgcttc aacacgctcg
    29041 cgcgtgcgct ggctcacgcc gctttcgtta ttcagcaccc gggaaaccgt agatttcccc
    29101 acgccgctta agcgcgcgat gtctttgatg gtcagccgat tttgcatcct gttgtcccgt
    29161 aacgtgttgt ttaattattt gagcctaact ttacccgtgc attcagcaat gggtaaagtc
    29221 tggtttatcg ttggtttagt tgccagcagg tattatatcg ccatagatgc tacgaatatt
    29281 attggattct ccttattatt tgcggcactt ttttcactta ccggaggtta tatggaacct
    29341 gatcccacgc ctctccctcg acggagatta aaacttttcc ggtaagcccg tcttttcacg
    29401 gcgttaccgg atgcgtaagg ccgtgacgtt ttaacgtccc agctcagctt tattaccttc
    29461 aggtaaggct tcgccacgcc gaagacattt ctgtactgtt tcagacagta cggagggact
    29521 ccttatgttt aaagaaattt ttacccggtt cattcgccat ttaccttccc gtctggttca
    29581 tcgcgatcca ttgcctggcg cgcagcagac agtgaatacg gcggtcccgc cgtccttaag
    29641 tgcgcattgc ctgaaaatgg cggtgatgcc cgaagaagaa ttgtggaaaa cgttcgacac
    29701 ccatccggaa ggattaaatc aggcggaagt ggaatctgcc cgcgaacaac atggtgaaaa
    29761 taaattaccc gcacaacaac cgtcgccgtg gtgggtacat ttatgggtct gctatcgcaa
    29821 cccctttaat attttactca ccattctcgg cgctatttct tacgccacgg aagatttatt
    29881 tgccgcgggc gttatcgcgc taatggtcgc tatttctacg ttgctgaact ttattcagga
    29941 agcacgttcc actaaagcgg cagatgccct gaaagcgatg gtcagcaata ccgcgacggt
    30001 gctgcgcgta attaacgaca aaggcgaaaa tggctggctg gagatcccga tcgaccagct
    30061 ggtgcccggc gatattataa aactggcggc gggggatatg atcccggcag atttacgtat
    30121 cttgcaggcg cgggatctgt tcgtcgctca ggcgtcgtta accggtgagt ctctgcccgt
    30181 ggaaaaagcc gctactactc gccagccgga gcacagcaat ccgctggagt gcgacaccct
    30241 gtgttttatg ggcaccaccg tggtgagcgg tacggcacaa gcaatggtga ttgccacagg
    30301 ggccaatacc tggtttggtc aactggcggg gcgtgttagt gagcaggaaa gcgagccgaa
    30361 tgcctttcag caagggatta gccgcgtcag tatgctgctg attcgcttta tgctggtgat
    30421 ggctccggtg gtgttgctta tcaacggtta caccaaaggc gactggtggg aagcggcact
    30481 gtttgcgctt tcggtcgcgg taggcttaac gccagaaatg ttgccgatga ttgttacctc
    30541 gacgctggcg cgcggcgcag taaagctgtc gaaacagaaa gtgatcgtca agcatctgga
    30601 tgccattcag aactttggcg caatggatat tctgtgcact gataaaaccg gcaccctgac
    30661 acaggataaa attgtgctgg agaatcatac cgatatctcc ggtaaaacca gcgaacgcgt
    30721 gctgcatagc gcgtggttga acagtcatta ccagaccgga cttaaaaacc tgcttgatac
    30781 cgcggtgctc gaaggtacgg atgaagagtc agcgcgttcg ctggccagcc gttggcaaaa
    30841 aattgatgag attccgtttg atttcgagcg tcgtcggatg tcggtggtag tggcagaaaa
    30901 taccgaacac catcagctgg tttgcaaagg tgcattgcag gaaatcctca atgtgtgttc
    30961 gcaggtgcgt cacaatggcg agattgtgcc gctcgatgac atcatgctgc gtaagattaa
    31021 gcgggttact gatacgctga atcgtcaggg gctgcgcgtg gttgcggtgg cgacgaaata
    31081 cctgccagcg cgtgaaggag attaccagcg ggcggatgaa tccgacctga tcctcgaagg
    31141 atatattgct ttccttgatc cgcccaaaga gacaactgct ccggcactga aggcattaaa
    31201 agcgagtgga attaccgtaa aaatccttac tggcgacagt gagttagttg cagcgaaagt
    31261 gtgccatgaa gtggggctgg acgcgggaga ggtggtcatt ggtagtgata ttgaaacgct
    31321 atctgacgac gaactggcaa atctcgcaca gcgcaccacg ctgtttgctc gcctgacgcc
    31381 gatgcataaa gaacgcattg tgaccttact gaagcgcgaa gggcatgtgg ttggctttat
    31441 gggcgatggt attaatgatg cgcccgcctt acgtgctgcg gatatcggca tttctgtgga
    31501 cggcgcggta gatattgccc gtgaagcggc tgatatcatc ctgctggaaa aaagcctgat
    31561 ggtgctggaa gagggggtta ttgagggacg tcgcactttc gccaacatgc tgaaatacat
    31621 caaaatgacg gcgagctcta acttcggtaa tgtgttcagc gtgctggtgg cgagtgcttt
    31681 cttgcccttc ctgccgatgt tgccgttaca cttgcttatt cagaacctgc tgtacgatgt
    31741 gtcacaggtg gcgatcccgt ttgataacgt cgacgacgag caaattcaaa agccgcagcg
    31801 ttggaatccg gcggatctgg ggcgctttat gatcttcttc ggaccgatca gctcgatctt
    31861 cgatattctg acgttttgcc tgatgtggtg ggtattccat gccaacacgc cggaaacgca
    31921 aacgctgttc cagtcgggct ggtttgtggt gggattactg tcgcaaacgc tgattgtgca
    31981 tatgatccgc acccgtcgtg taccgtttat tcagagctgt gcatcgtggc cgttaatgat
    32041 catgaccgtg atcgtgatga ttgtcgggat cgtattgccg ttttcaccgc tggccagtta
    32101 tctgcagctg caggcgctgc cgttgagcta tttcccgtgg ctggttgcga ttctggcagg
    32161 gtatatgacg ttaacccagt tggtgaaagg gttctatagc cgtcgttacg gttggcagta
    32221 aagaataaac tgggcatggt ggcccggaat cagattgcag acaaagtgag ctttgttcat
    32281 gccggatgcg gcgtaaccgc ctaatccggt ctacaaattc aataaattgc aggaatcacg
    32341 taggcctgat aagcgtagcg catcaggcga ttttgctttt gtcatcagac ttaatccggg
    32401 catgatagcc cggatttcca tcaagattag cgacgaacag cgatcgcttc gatctcaatc
    32461 ttcacgtctt tcggcagacg ggctacttca acgcaagaac gtgccgggaa ggtggcgttg
    32521 tgttcggtga agaaggcttc gtaggtagca ttaacggttg cgaagtcgtt cagatctttc
    32581 acaaacacgg tagttttaac gatgtcgccc actttcaggc cagcggcttc gacgattgct
    32641 tttacgttat ccagcgactg acgtgcctgt gcagcgacgt ctgccgggac ttcacccgtt
    32701 ttcggattta ccgggatctg accggaggtg atgatcatat tgcccagatc aacgccctgt
    32761 acgtaaggac cgatagctgc cggtgcattt tccgtcgcga tagttttgct catgatttct
    32821 cctttattac agcggtaaaa agtctggctc attataggga gccagacttt tattaccaac
    32881 cgcaattaat tggccagcac cacattatgg gaaaactctt tttcacagta tttgcatttg
    32941 agcgcgatgt cattggcgcg ttttcgcacg gcaaagctgg atgaaaccgg ttcggcatgg
    33001 ctgatacagt tgctgttcgg gcagaccagc acattgtcga tgcgctccgg cagactgggg
    33061 cgcgatttgc caaccacttc atagttgtcg atacggttaa ccgtggcttg cggtgcatac
    33121 aatgccagtt gatctacctg atcttcactc aaaaaggtat tttcgatttt gatcagatct
    33181 ttgcggccca tctcgccaga aggcaggttc agaccaatgg tgatccgctg atccgtttcg
    33241 gtcagcttga acagactcaa cagcttaaaa ccgatctggg cggggatatg gtcaattacc
    33301 gtgccgcgtt taatagcttc aacctgcaat ttattatcgt gtgtcatctc tatttcccct
    33361 tacagtacca gatcgcgatt cagaaccagt gccagtaacg cctggcgagc gaaaatcccg
    33421 ttgcctgctt gctggaagta ccaggcgtga ggcgttttat ccacatccgt cgcaatctca
    33481 tcaacacgcg gcagcggatg cagcactttc atattggctt tggcgttgtg gagatcgctg
    33541 gcgcgaagaa caaactgcgc tttcacgttg gcgtactcgg acgggtccag acgctctttt
    33601 tgcacgcgag tcatgtacag gatgtctact tccgccatca cttcttcaat agagctgtgc
    33661 agactccagg cgatcccttt ttcatcgagc atatcaagga tgtattgcgg cattgccagc
    33721 gcgtccggcg cgatgaagta aaaacggttg ccgtcgaact tcgctaacgc ctgagtcagg
    33781 gagtgaacgg tgcggccata tttcaggtca ccaaccattg cgacgtggag attgtccaga
    33841 cgcccctggg tttcctgaat ggtgaataag tccagcaagg tttgcgtcgg atgttggttg
    33901 gagccatcac cggcattcag taccggtaca ttgccagaaa attcggtggc caggcgcgcc
    33961 gccccttcct gcggatgacg catcactatc gcatcgacgt aagtgctgat aaccgaaatg
    34021 gtatcggcca gcgtttcgcc ttttttaccc agtgatgtat tggcgctgtc ggagaagccc
    34081 accacgctgg cacccaggcg gtgcatggaa gtttcgaaag agaggcgggt acgggtagag
    34141 gcttcgaaga aacagctggc aatgactttg tgcttcaaca gctctggttg cgggtttgct
    34201 ttcagtttcg ccgctgtcgc cagcaccaga ttaaggtcat cgcgactaag gtcgtttatg
    34261 gaaatgatat gtttctgata tagcggatta gccatctttt atctcctgac gcctgggcaa
    34321 aaaaaagccc ctcgattgag gggctgggaa tgggtgatca acgggaagaa aaacggcagg
    34381 ccagcgtctt ttttcagacg cggtaagaca aaatgtcgaa cacactgaac catacatcct
    34441 cccggcaaat tgtccggcat tatactcata gccagaagcg gtgcaagcat ttgatgcaat
    34501 attttgtcag cgcaaacggt ttatttgaat taaaagtcaa aatatatgca tttttatttt
    34561 ctatgattct ggaggggaag tgtcgttcgg tatcaataat tgcagacatt tcctgctttt
    34621 ttctttttca ttttcacgca atcagaaatg cattttattc cattcatccg acttataggg
    34681 cggagggtga aaacgaacgg ctaacactat tgcttatagc tcagggatac gcgctatcac
    34741 tttaatttca aaatcaaagc ctgccagcca ggtaacaccc accgccgtcc agtttggata
    34801 aggtggggcg ctaaatattt catttttcac cgtcatgatg tcttcaaatt gtttttccgg
    34861 atcggtatga aagctcgtaa catcaatgat atcgtcaaaa gtgcatcccg cagctgccag
    34921 ggtcgcatgc aaattatcaa aggccagtct gacttgttgc tgaaaatcgg gttctggtgt
    34981 tccgtcctct cgacttccta cttgcccgga aacaaacagc aaatcgccgg aacgaatagc
    35041 cgcagaataa cgatgctcag catacagtga atgtcggcca gcagggaaaa cagcggttct
    35101 ttctaccatt tggttatcct caagatttac gacatgaaca gaagatttat atttaccggg
    35161 agccgctttt agggggacga cgttagtaaa caaaacccag acatcatgga taatggctgg
    35221 gcttaattga gcgtagtcgg ttatgcgcca aacgcgccat caatggtatg catcgcgccg
    35281 gtaacaaaac tggcttctgg ccctgctaac catgcgacca taccagcgac ctcttccggt
    35341 tgcccatgtc ttttgatggc cataaaacta tgcaacatat cgcgcattgg cccgttggcg
    35401 ggattagcgt cggtatcaat tggcccaggc tggacgacgt taatggtgat ccctcgcggt
    35461 ccaaaatcac gggccagccc gcgtgccatg ccttgcaggg cagatttact ggcggcataa
    35521 gcagccatgc ctgcaacagg catacgatcg ccattcacgg agccgatgat taagatacgc
    35581 ccgccttcgg gcatctgccg ggcggcttca acagaggcat gataaggagc atgaatattg
    35641 attttgaaaa ggcgatcaat atcgtcggca tttaattcca gggcatcgcc aaagacgcca
    35701 atacctgcat ttaccaccag gatatccaat gcgccgctct tacgaacgac atcaatgaca
    35761 gcgtctctgt cagcactatc tgtgaatact gctgtcgctc cagtctcttg tgccaggcgt
    35821 tcagcggcat ctttcgaccc cgcataggtg aatcgtacat tggccccatc ggtgacgaaa
    35881 cgacgtacga tagcggcacc gattccacgg ctgccaccga ggaggagaac tgtcttaccg
    35941 gtaaaagcgc ccataaggac tccttgattt attatgtaac atgcattaca aaactgtttt
    36001 aactttctgt caacaagttt tgtaatggtc actaaaaaac aatctcgtgt tccaggtcgc
    36061 cccagacgtt tcgctcctga tcaggcagtc tctgcggcaa aagtgctttt tcatcaaaaa
    36121 ggtttcgatg ctgtcagtgt tgctgaagtt actgattatc ttggtattaa ccccccgagc
    36181 ctctacgcgg cttttggcag taaggctggg ttatttagcc gtgtactcaa tgaatatgtc
    36241 ggtacggaag ctattccgct tgccgatatt cttcgtgatg atcgtccggt aggcgagtgc
    36301 ctggttgagg tattaaaaga agcggcgtgc agatatagcc aaaacggcgg ctgcgctggc
    36361 tgtatggttc ttgaaggtat tcatagtcat gatccacaag cgcgtgatat tgccattcaa
    36421 tattatcacg ccgcagaaac gaccatttat gactatatcg ccaggcggca tccacaaagc
    36481 gcacaatgtg tgactgattt tatgagtacc gtgatgtcag ggctttctgc gaaggcacgg
    36541 gaggggcact ccatcgaaca actctgtgca acagctgccc tggcggggga agcgataaaa
    36601 actcttctca aggagtgatg ctggccttga tccgaaaggc gggaacgcgc ctgccgataa
    36661 gttgtgataa gacaataatt cacgcgttaa ggctggcgga attgatcatc ttttcgtata
    36721 acgatagaaa tgaaacattg ttttaattaa ggagtggaaa agatgatcat cggaaatatt
    36781 cataatcttc agccgtggct accccaagag ttacgccagg cgattgagca tatcaaagca
    36841 aacgttacgg cagaaacgcc caagggcaag cacgatatcg aaggcaatcg tctgttttat
    36901 cttatctcgg aagatatgac cgagccgtac gaagctcgcc gtgcggagta ccatgcccgc
    36961 tatctcgaca ttcagattgt gttaaaaggt caggaaggca tgaccttcag cacacaacct
    37021 gcaggtacgc cggataccga ctggttagct gataaagaca tcgcattttt gccggaaggc
    37081 gttgatgaga aaacagttat ccttaatgaa ggtgattttg ttgtgtttta tccgggggaa
    37141 gtgcataaac cgctgtgcgc agtgggcgcg ccagcccggg ttcgcaaagc agtagtgaag
    37201 atgctgatgg cgtgatgact tttcgccgta aataactcca ggtttacggc gagtttgtga
    37261 aaagagcgtt ttttgatatt tttttgtgag taaaatttgt aatgcttaga cgttcttatt
    37321 aactcaagga gttcgtcatg agcaaaatat cagatttgaa ttattctcaa cacattacat
    37381 tagccgacaa ttttaaacaa aaaagtgaag ttttaaatac ctggcgtgtt ggaatgaata
    37441 attttgcccg tattgccggg gggcaggata gcagaagaaa tatccttgat cctaagacat
    37501 ttttggagtt tttggtaaaa atatttaccc ttggttatgt ggattttagc aaacgctcca
    37561 acgaagcggg aagaaatatg atggctcata ttgagtcctc atcttatatc aaaaataatg
    37621 atggcagtga gataatgaag tttgttatga ataatcctga aggggaacga gcggatttat
    37681 ctaaggtgga aatagaaatt acactttcca ctatcactac tatggggact cgtcaaggac
    37741 atacggctat tatatttcaa caatctgatg gttcgactaa tcgttatgaa gggaagtcct
    37801 ttgaaagaaa agatgagagt tcattacacc tgattactaa caagattctg gcgtgttacc
    37861 aacgcgaagc taacaag
//
LOCUS       DAPHCV010000040        37625 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.040, whole genome shotgun sequence.
ACCESSION   DAPHCV010000040 DAPHCV010000000
VERSION     DAPHCV010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.040"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            227..1597
                     /gene="aroP"
                     /locus_tag="RH033_001987"
     CDS             227..1597
                     /gene="aroP"
                     /locus_tag="RH033_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HDV2991175.1"
                     /translation="MEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSAG
                     PGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVLY
                     VLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIKV
                     IAVVAMIIFGAWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLEL
                     VGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIFH
                     ELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPVN
                     TILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQGV
                     VTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKTA
                     KAVKAH"
     gene            complement(1640..2494)
                     /gene="ampE"
                     /locus_tag="RH033_001988"
     CDS             complement(1640..2494)
                     /gene="ampE"
                     /locus_tag="RH033_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HDV2991176.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQIDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2491..3042)
                     /gene="ampD"
                     /locus_tag="RH033_001989"
     CDS             complement(2491..3042)
                     /gene="ampD"
                     /locus_tag="RH033_001989"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HDV2991177.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLVAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3130..4023
                     /gene="nadC"
                     /locus_tag="RH033_001990"
     CDS             3130..4023
                     /gene="nadC"
                     /locus_tag="RH033_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HDV2991178.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANE
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4226..4666
                     /gene="ppdD"
                     /locus_tag="RH033_001991"
     CDS             4226..4666
                     /gene="ppdD"
                     /locus_tag="RH033_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HDV2991179.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            4676..6061
                     /gene="gspE"
                     /locus_tag="RH033_001992"
     CDS             4676..6061
                     /gene="gspE"
                     /locus_tag="RH033_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HDV2991180.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAIHEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVLPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTTETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRLQQGEPIHIPDNVWPSPLPRWQAPGCVHCYHGF
                     YGRTALFEVLPITPFIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6051..7253
                     /gene="hofC"
                     /locus_tag="RH033_001993"
     CDS             6051..7253
                     /gene="hofC"
                     /locus_tag="RH033_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HDV2991181.1"
                     /translation="MASKQLWRWRGITGGGNAQDGMLWAESRALLLIALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLADKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPVLTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRATMRCPYWEQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLARHHRDNTMALADNLAALLEPVLLVITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7288..8331)
                     /gene="guaC"
                     /locus_tag="RH033_001994"
     CDS             complement(7288..8331)
                     /gene="guaC"
                     /locus_tag="RH033_001994"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDV2991182.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8487..8531)
                     /locus_tag="RH033_001995"
     CDS             complement(8487..8531)
                     /locus_tag="RH033_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2991183.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8556..9176
                     /gene="coaE"
                     /locus_tag="RH033_001996"
     CDS             8556..9176
                     /gene="coaE"
                     /locus_tag="RH033_001996"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDV2991184.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ASSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9176..9919
                     /gene="zapD"
                     /locus_tag="RH033_001997"
     CDS             9176..9919
                     /gene="zapD"
                     /locus_tag="RH033_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HDV2991185.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPVVDYAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            9929..10126
                     /gene="yacG"
                     /locus_tag="RH033_001998"
     CDS             9929..10126
                     /gene="yacG"
                     /locus_tag="RH033_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HDV2991186.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10328..10726)
                     /gene="mutT"
                     /locus_tag="RH033_001999"
     CDS             complement(10328..10726)
                     /gene="mutT"
                     /locus_tag="RH033_001999"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HDV2991187.1"
                     /translation="MKKLQIAVGIIRNENNKIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEILEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVLAKLKRVYVG"
     gene            complement(10786..13491)
                     /gene="secA"
                     /locus_tag="RH033_002000"
     CDS             complement(10786..13491)
                     /gene="secA"
                     /locus_tag="RH033_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDV2991188.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13553..14065)
                     /gene="secM"
                     /locus_tag="RH033_002001"
     CDS             complement(13553..14065)
                     /gene="secM"
                     /locus_tag="RH033_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HDV2991189.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(14295..15212)
                     /gene="lpxC"
                     /locus_tag="RH033_002002"
     CDS             complement(14295..15212)
                     /gene="lpxC"
                     /locus_tag="RH033_002002"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HDV2991190.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15313..16464)
                     /gene="ftsZ"
                     /locus_tag="RH033_002003"
     CDS             complement(15313..16464)
                     /gene="ftsZ"
                     /locus_tag="RH033_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDV2991191.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16525..17787)
                     /gene="ftsA"
                     /locus_tag="RH033_002004"
     CDS             complement(16525..17787)
                     /gene="ftsA"
                     /locus_tag="RH033_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDV2991192.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17784..18614)
                     /gene="ftsQ"
                     /locus_tag="RH033_002005"
     CDS             complement(17784..18614)
                     /gene="ftsQ"
                     /locus_tag="RH033_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HDV2991193.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18616..19536)
                     /gene="ddlB"
                     /locus_tag="RH033_002006"
     CDS             complement(18616..19536)
                     /gene="ddlB"
                     /locus_tag="RH033_002006"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDV2991194.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19529..21004)
                     /gene="murC"
                     /locus_tag="RH033_002007"
     CDS             complement(19529..21004)
                     /gene="murC"
                     /locus_tag="RH033_002007"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDV2991195.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDKAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(21058..22125)
                     /gene="murG"
                     /locus_tag="RH033_002008"
     CDS             complement(21058..22125)
                     /gene="murG"
                     /locus_tag="RH033_002008"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDV2991196.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22122..23366)
                     /gene="ftsW"
                     /locus_tag="RH033_002009"
     CDS             complement(22122..23366)
                     /gene="ftsW"
                     /locus_tag="RH033_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HDV2991197.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23366..24682)
                     /gene="murD"
                     /locus_tag="RH033_002010"
     CDS             complement(23366..24682)
                     /gene="murD"
                     /locus_tag="RH033_002010"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDV2991198.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGGLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKICVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24685..25767)
                     /gene="mraY"
                     /locus_tag="RH033_002011"
     CDS             complement(24685..25767)
                     /gene="mraY"
                     /locus_tag="RH033_002011"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDV2991199.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLVGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25761..27119)
                     /gene="murF"
                     /locus_tag="RH033_002012"
     CDS             complement(25761..27119)
                     /gene="murF"
                     /locus_tag="RH033_002012"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDV2991200.1"
                     /translation="MISVTLSQLTDILNGELKGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVNRPLDIDLPQVIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHIQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFSDKTALIARLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27116..28603)
                     /gene="murE"
                     /locus_tag="RH033_002013"
     CDS             complement(27116..28603)
                     /gene="murE"
                     /locus_tag="RH033_002013"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDV2991201.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28590..30356)
                     /gene="ftsI"
                     /locus_tag="RH033_002014"
     CDS             complement(28590..30356)
                     /gene="ftsI"
                     /locus_tag="RH033_002014"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HDV2991202.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMAEYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTVPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30372..30737)
                     /gene="ftsL"
                     /locus_tag="RH033_002015"
     CDS             complement(30372..30737)
                     /gene="ftsL"
                     /locus_tag="RH033_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDV2991203.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30734..31675)
                     /gene="rsmH"
                     /locus_tag="RH033_002016"
     CDS             complement(30734..31675)
                     /gene="rsmH"
                     /locus_tag="RH033_002016"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDV2991204.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31677..32135)
                     /gene="mraZ"
                     /locus_tag="RH033_002017"
     CDS             complement(31677..32135)
                     /gene="mraZ"
                     /locus_tag="RH033_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDV2991205.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32737..33741)
                     /gene="cra"
                     /locus_tag="RH033_002018"
     CDS             complement(32737..33741)
                     /gene="cra"
                     /locus_tag="RH033_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HDV2991206.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33823..33909)
                     /locus_tag="RH033_002019"
     CDS             complement(33823..33909)
                     /locus_tag="RH033_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HDV2991207.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(33921..34412)
                     /gene="ilvN"
                     /locus_tag="RH033_002020"
     CDS             complement(33921..34412)
                     /gene="ilvN"
                     /locus_tag="RH033_002020"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDV2991208.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34415..36139)
                     /gene="ilvI"
                     /locus_tag="RH033_002021"
     CDS             complement(34415..36139)
                     /gene="ilvI"
                     /locus_tag="RH033_002021"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HDV2991209.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTSGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESVHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            36258..36302
                     /locus_tag="RH033_002022"
     CDS             36258..36302
                     /locus_tag="RH033_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2991210.1"
                     /translation="MKRNDKILHYRGLN"
     gene            complement(36457..37401)
                     /gene="leuO"
                     /locus_tag="RH033_002023"
     CDS             complement(36457..37401)
                     /gene="leuO"
                     /locus_tag="RH033_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HDV2991211.1"
                     /translation="MPEVQTDHPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQTWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         9098 a        10462 c         9144 g         8921 t
ORIGIN      
        1 tgaggctgac ctttacaaac ggttcctttt taagcaactc atcttcaacc atgcataaag
       61 cgggtgcatt cgctgccgta taccattatt ctttatctga cggaagtcct tttgtaacaa
      121 ttcaaacgtc tttgatgtaa acaaattaat acaaaaaacg gaattgcaaa cttacacacg
      181 catcactgcg tagatcaaaa aaacaatcac cgcacgaggt ttcataatgg aaggtcaaca
      241 gcacggcgag cagctaaagc gcggccttaa aaaccgccat attcagctta tcgcgctggg
      301 tggcgcgata gggacagggc tattcctggg tagcgcctcc gtaatacagt ccgcagggcc
      361 agggattatc ctgggttacg ccattgctgg ttttatcgcc tttctgatca tgcgtcagct
      421 aggtgaaatg gtggtcgaag aacctgtcgc aggctccttt agccactttg cttataaata
      481 ctggggcagt tttgccggtt tcgcctctgg ctggaactac tgggtactgt acgttttagt
      541 tgccatggca gagctgactg ccgtgggtaa atacattcag ttctggtatc cggaaatccc
      601 aacctgggtt tctgccgccg tgttctttgt ggtgatcaac gccatcaacc tgacaaacgt
      661 aaaagtgttt ggcgagatgg agttctggtt tgccattatc aaagttatcg cggtggtagc
      721 gatgatcatc ttcggtgcct ggctgctatt cagcggcaac ggcggcccgc aggcgagcgt
      781 tagcaacctg tgggatcagg gtggtttcct gccacacggc ttcaccggtc tggtgatgat
      841 gatggcgatt atcatgttct ctttcggtgg tctggaactg gtggggatca ccgcagcaga
      901 agctgataac ccggagcaaa gtatcccgaa agcaactaac caggttatct accgcatcct
      961 gattttctat attggttcgt tagccgttct gctctcacta atgccgtgga cccgcgttac
     1021 cgccgatacc agtccgtttg tgctgatctt ccacgagtta ggcgatacct ttgtagcgaa
     1081 tgcgctgaat atcgtggtac tgactgctgc gctctccgtg tacaacagct gcgtatattg
     1141 caacagccgt atgctgttcg gtctggcaca acaagggaac gcgccaaaag cgctggcgtc
     1201 tgtcgataaa cgcggcgtac cggttaacac tattctggtg tctgcgctgg ttacggcatt
     1261 gtgcgtactg attaactacc ttgccccaga gtccgctttc ggactgttaa tggcgctggt
     1321 ggtatctgca ctggtcatca actgggcgat gatcagtctg gcgcatatga aattccgtcg
     1381 tgccaagcag gaacaaggcg tggtaactcg cttccctgct ctgctttatc cgctgggtaa
     1441 ctggatctgc ctgctgttta tggcggcggt actggtgatt atgctgatga ccccaggaat
     1501 ggcgatttcg gtatacctga tcccggtatg gctgatcgtg ttaggtatcg gctatctgtt
     1561 taaagagaaa accgccaaag ccgtaaaagc gcattaatct ctctacgccc tcacccgtgc
     1621 agggtgaggg caataatctt tacaccaacg ccccgtaaat cgtcagtagc gcaattacca
     1681 ccacgaccac gaacgaggtt ttcttcgcca ttgaaaccgc tgcctttggc gtctccacct
     1741 tatcgacatg cggttcacgc gccagagaga actgtgccag acgcgttaat acctgatatt
     1801 gcgaagtatg gaaatcaccc agcgaagcaa accaggccgg taacgctttc tcaccatgac
     1861 cgatcaaggc atataccaca cccgcaagac gaaccggcac ccaatccagt acatgaagca
     1921 cggcatcaat gccggactgt aaacgatgat gcggcgtctg gtatcgcgcc agccagtatt
     1981 gccatgcacg caaaaacgca taccccatca gcgtaacggg tccccaggtt ccccccacaa
     2041 tcagccagaa cagcggagca agataaaaac gaaagttaat ccacagcaat gcattttgca
     2101 gctcacgcaa atactcacgt tcatcgcagc ctgccgggac gccgtgaatc atggtgagtt
     2161 cgccagccat cgtggcacgg gcatggctat cattacgtga agcagctgtc agataagcat
     2221 gataatgaag acgaacttta cctgcgccaa tacacagcaa accaatcagc agccacacca
     2281 gcagcgtggg aacgttgaac aatactccct gcaatgcgcg taacagtaaa aaagtcacgc
     2341 ccatcgcaat aatggtcatg cctaatgtgc gcccgagaga aaaatgtttc acccgccgaa
     2401 agaacgcttc aagacgatga tcaatctgcc agtgctcgcc caacttaaac aggcgctcga
     2461 aaattaacac cagtaaggtt gtaaatagcg tcatgttgtc tccttgctga ccagtgcacg
     2521 aaaccgtgcc caatcaaatg caggaccggg atcggttttc cgatccggcg caatatcaca
     2581 atggcccgtc atgttattag cgatatctgg atagcgatca atcagtgccc gcgtaaccgc
     2641 cacaagctgt tgatactgcg catcggtata cgccagcgta tcggtgcctt ccagctcaat
     2701 cccaatagaa aaatcattgc agcgttcgcg cccctgatac tgagagactc cggcatgcca
     2761 ggcacgttta tcgaaaggaa catactggac tatttcacca tcgcggcgaa tcaaacagtg
     2821 agcggagacg cgcaaatggg cgatctcagc aaagaaagga tgcgcctgcg gatcaatagt
     2881 tccagtgaat aatgcgtcga tccacggacc gccaaactcg cctggcggca ggctaatatt
     2941 gtgcaccacc agcagggtgg gtgtttcgtc atccgggcgg caatcgtaat gtggtgaggg
     3001 aacgcggcgc gcgccaacca gccacccctg ttctaacaac atgcaggatc tccttatatg
     3061 tggtgctaat acccggttca gagtagcatg tttctacctt atgattcgtt agctatctgg
     3121 agttttaaca tgccgcctcg ccgctataac cctgacaccc gacgtgacga gctgctggaa
     3181 cgcattaatc tcgatatccc cggcgcggtg gcccaggcgc tgcgggaaga tttaggcgga
     3241 acagtcgatg ccaacaatga cattacggca aaacttttac cggaaaactc tcgctctcat
     3301 gccacagtga tcacacgcga gaatggcgta ttttgcggca aacgctgggt tgaagaggtg
     3361 tttattcaac tggcaggcga cgatgtcacc ataatctggc atgtggatga cggcgatgtc
     3421 atcaacgcca atgaaccctt gttcgaactt gaaggcccat cccgcgtgct gttaacgggc
     3481 gaacgcactg cacttaattt tgtgcaaacc ctttcagggg ttgccagtaa ggtacgccac
     3541 tatgtcgaat tactggaggg caccaacacg cagttgttgg atacgcgcaa aaccttaccc
     3601 ggcctgcgtt cagctctgaa atacgcggta ctttgcggcg gcggagcgaa tcaccgcctg
     3661 gggctttctg atgccttcct gatcaaagaa aaccatatta ttgcctccgg ctcagtgcgc
     3721 caggcggtcg aaaaagcgtc ctggctgcac ccagatgcgc cagtagaagt cgaagtagag
     3781 aatctggaag aacttgatga agccctgaaa gcaggtgccg atatcatcat gctggataac
     3841 ttcgaaacag aacagatgcg cgaagccgtc aaacgcacca acggcaaggc gctactggaa
     3901 gtgtctggca acgtcactga caaaacactg cgcgaatttg ccgaaacggg cgtggacttt
     3961 atctccgtcg gtgcgctaac taaacacgta caggcactcg acctttcaat gcgttttcgc
     4021 tgattgctga cagatttatc cgctccatcg cgggcggata atctcccctt tcccaacttt
     4081 cttcgtaacg cctcgcaaat ttcttgtctt tcgctgcaac gtaataaaaa actccggaag
     4141 ctgctttccc gtttcgtttt ttgcccctcg cttgtgctga tggcgtttgc caaagtagcg
     4201 ccaaccaaat caaggagcga aacaaatgga caagcaacgc ggttttacac ttatcgaact
     4261 gatggtggtt attggcatca ttgccatttt aagcgccatt ggtattcccg cttatcaaaa
     4321 ctacctgcgc aaagccgcac tcaccgacat gctacaaacc tttgtgcctt accgtaccgc
     4381 cgtagagttg tgcgcgctgg aacatggtgg attagatacc tgcgacggtg gcagcaatgg
     4441 cattccctcg cctaccacca cccgctatgt ttcagccatg agtgtggcaa agggcgtggt
     4501 gtcgctgacc gggcaagaaa gtctcaatgg gctaagcgtc gtcatgacac cgggttggga
     4561 taacgcaaac ggcgtcaccg gctggacgcg caactgcaat attcaaagtg acagcgcatt
     4621 gcagcaagcc tgcgaagatg tctttcgctt tgatgacgcc aactaaggag cggcaatgaa
     4681 tattccacag ctcaccgccc tgtgtctgcg ttatcaggga gtcttgctgg atgccagcga
     4741 agaggtggtt catgttgcgg tggtcgatgc cccttcgcat gagctactgg acgcattgca
     4801 tttcgctacc accaaacgta ttgagatcac ctgctggacg cgccaacaaa tggaaggtca
     4861 cgccagtcgc acccaacaga cattgcccgt agctattcat gagaagcatc agcccaaagc
     4921 agagttgctg actcgaacgt tacaatctgc gctggaacaa cgcgcgtctg atattcatat
     4981 cgaaccagcg gacaatgcct accgcatccg cttgcgtatc gacggtgtat tgcatccttt
     5041 accggatgtt ttaccggatg ccggagtcgc attaaccgcc agattaaaag tgctgggcaa
     5101 cctggatatt gcggaacatc gcctgccgca ggacgggcaa ttcactgtcg aactggcagg
     5161 aaacgccgtc tcatttcgta ttgcgacctt accatgccgg ggtggtgaaa aggtggtatt
     5221 aaggttgtta cagcaggtga gccaggcact ggatgtcaac acgctgggga tgcagccgtt
     5281 acaactggcg gactttgctc atgccttgca acaaccacag ggactggtgc tggtaactgg
     5341 ccctacaggc agcggcaaaa cggtcacgct ttatagtgcc ctgcaaacgc tgaataccgc
     5401 tgacattaat atttgtagcg tcgaagatcc ggttgagatc cccatagccg gactaaacca
     5461 gacgcaaatc catccgcgtg ccggtcttac ctttcagggc gttttgcgtg cgttattgcg
     5521 ccaggaccct gacgtcatca tgatcggaga gatccgcgat ggcgaaacag cagagatcgc
     5581 tattaaagcg gcgcaaaccg gtcacctggt gttgtctacc ctacacacca attccaccac
     5641 tgaaacactg gtacgtttac agcaaatggg agtcgcccgc tggatgctct catcggcgct
     5701 tacgctggta atagcccagc gtctggtacg caaactttgc ccacattgcc gcctgcagca
     5761 aggggagccc atccatattc cagacaatgt atggccgtcg ccgctgcccc gctggcaagc
     5821 acccggttgt gtacattgct accacggttt ttatggtcgc acggccttat ttgaagttct
     5881 gcccataaca ccgttcatac gtcagcttat ttccgctaat accgacgttg aatcgctgga
     5941 aacgcacgcc cgacaggcgg gtatgcgaac gctttttgaa aacggctgcc tggccgtgga
     6001 gcaaggctta accacctttg aagagttaat ccgcgtattg ggaatgccgc atggcgagta
     6061 agcaactctg gcgctggcgt ggcataaccg gcggcggcaa tgcgcaagat gggatgctat
     6121 gggcagagag ccgtgctttg ctgctcatag cactacagca acagatggtt accccactta
     6181 gcctgaagcg aatcgccatc aattctgcgc agtggcgagg agataaaagc gcggaagtca
     6241 ttcatcaact ggcgacgcta ctcaaagccg ggttaacgct ttctgaaggg ctggcactgc
     6301 tggcggaaca gcatcccagt aagcaatggc aagcgttgct gcaatcgctg gcgcacgatc
     6361 tcgaacaggg cattgctttt tccaatgcct tattaccctg gtcagaggta tttccgccgc
     6421 tctatcaggc aatgatccgc acgggggaat tgactggtaa gttagatgaa tgttgctttg
     6481 aacttgctcg acagcaaaaa gcccaacgtc agttagccga taaagtgaaa tcagcgttgc
     6541 gttatcccat catcatttta gcgatggcaa tcatggtggt tgtggcaatg ctgcattttg
     6601 tgctgccgga gtttgctgct atttataaga ccttcaacac cccactaccg gtactaacgc
     6661 aggggatcat gacgctggca gactttagtg gcgaatgggg ctggctgctg gtgttgttcg
     6721 gctttctgct ggcgatagcc aataagttgc tgatgcgccg cccgacctgg ctcatcgtga
     6781 ggcaaaaatt actgttacgc atcccaatta tgggttcact gatgcgagga caaaaactca
     6841 cgcagatctt tacgattctg gcgctgacac aaagtgcagg cattactttt ttacagggcg
     6901 tagagagcgt cagagcaaca atgcgctgtc cgtattggga gcaacttctg acacaaatcc
     6961 agcacgatat cagtaacggt catcccatct ggctggcgct aaaaaatgcc ggtgagttta
     7021 gcccgctctg tttgcaatta gtgagaacag gagaggcatc cggttcgctg gatctcatgt
     7081 tagacaacct cgcccgtcat catcgggata acaccatggc gctggcggat aaccttgcag
     7141 ccttgctcga accggtgttg ctggtcataa cgggaggaat tatcggtacg ctagtggtgg
     7201 caatgtattt gccaattttc catttaggcg atgcgatgag tgggatggga taacgctggc
     7261 gtgttactcc acgccagcat tgggagatta cagattgttg aagatgcggt tttcttgttc
     7321 ctgtacacgg ataaacgtag tgcgcttggt cagctctttc aggcgtgaag ccccaacgta
     7381 tgtacaagct gaacgcaggc cgcccaaaat atcacgcgcg gtattttcaa ccgggcctcg
     7441 cagcggcagc ttaacggttt taccttctgc tgcgcgatat tccgcaacgc cgccaacgtg
     7501 acgtttcatc gcagactcgg agctcatgcc gtagaacagc ataaatttct cgccgttctc
     7561 ctcaacgatg cgaccgccgc tctcttcgtg gcccgccagc atgccgccaa gcatgacgaa
     7621 atcggcaccg ccgccaaagg ctttcgcgac atcgcccggc gtggtgcagc caccgtcgct
     7681 gacgatcatt ccgcccagac catgcgcagc atcggcacat tcgatcaccg cagaaagttg
     7741 cggataaccg acgcctgttt tgacgcgagt tgtacaaaca gaacctgggc caatgccaac
     7801 tttaacgata tcagcaccgg agaggataag ctcctcacac atttcaccag tcactacgtt
     7861 gccagcacaa atggttttgg tcggccacgc ttcacgcgct ttcgcaacga actgcacgaa
     7921 gtgttcagaa taaccattcg ccacgtcaat acaaacgaag tttaatgccg ggttcaggtc
     7981 gagaatctgt ttagtttttt cgaaatccgc atcagacgta ccggtagaaa ccatcacatg
     8041 tttcagcaca tcagcggaag aattgttgat aaacgcttgc cactcttcga cagaatagtg
     8101 tttatgcaca gcagtcagaa tatcaaaaga agccagcgca gaggccatag aaaatgtgcc
     8161 tacggtgtcc atatttgcgg caataatcgg cacgccggac cagctctgac ctgaatgttt
     8221 aaaggtgaat tgacgttcca gttcaacatc ggaacggctt ttaagagtgg agcgtttagg
     8281 gcggatgaga acgtctttaa aacctaactt cagatcttct tcaatacgca tgtgcggatt
     8341 cctggggtta atggctataa gggtaagagc gcaactccag tgacgctatc atacgcacta
     8401 atcaatgcgg cgcaagactg cgaaattgtc tttttttacg ctacaatccc ataaatttac
     8461 ctggtaaata gtgagcgaac gtttcatcaa tttcgctttc gtgcgctcat ttttaacgtt
     8521 ttgataatca tgatgaattc ccgggaatat aaattatgag gtatatagtt gccttaacgg
     8581 gaggcattgg cagtggcaag agtaccgttg ccaatgcgtt tgctgatctc ggaattaacg
     8641 tcattgatgc cgatattatt gcgcgtcagg tggttgaacc aggtgcacct gcgctacatg
     8701 ccattgctga tcactttggc gctaacatga ttgctgctga tggaacattg cagcgccggg
     8761 ccttgcgcga gcggattttc gccaacccgg aagagaaaaa ctggcttaac gccctgctgc
     8821 atccgctgat tcagcaagag acgcaacacc agatccagca agcatcttcc ccctatgtac
     8881 tttgggttgt gccattgctg gtagaaaact cattgtataa aaaagcgaat cgagtgctgg
     8941 tggtggatgt cagcccagaa acgcaactta aacgcaccat gcagcgcgat gatgtcactc
     9001 gcgagcatgt tgaacaaatt cttgctgctc aggcaacgcg cgaagcccgc cttgccgtgg
     9061 cagatgacgt cattgataat aacggcgcac cggatgctat cgcatcggat gttgcccgcc
     9121 tgcacgcaca ctatttgcag cttgcgtcgc agtttgtctc acaggaaaaa ccgtaatgca
     9181 gacccaggtc ctttttgaac atccactaaa tgaaaaaatg cgtacatggc tgcgcattga
     9241 gtttttaatt caacaactca ccgtaaattt gcccgtcgtt gactacgctg gcgctctgca
     9301 tttcttccgc aatgtcagtg aattgctgga tgttttcgag cgcggcgaag tccgcactga
     9361 gttgttgaaa gaacttgacc ggcagcaacg taaactccag acctggattg gcgtgcctgg
     9421 cgtggaccag agccgtattg aagcattaat tcagcagtta aaagcggcgg ggagcgtatt
     9481 aatttccgcg ccccgtatcg ggcaatttct gcgtgaagat cgtttgattg ccctggtgcg
     9541 tcagcgactg agcatccccg gcggctgttg tagctttgat ttacctacat tgcacatttg
     9601 gctgcatcta ccccaggcgc agcgcgacag ccaggtagaa acctggattg ccagcctgaa
     9661 cccgctcacc caggcactta ccatggtgct ggatttaatt cgccagtcgg cccccttccg
     9721 taaacaaacc agcctgaatg gtttttatca ggataacggt ggcgatgccg acttgctgcg
     9781 cctgaatctg tcactcgatt cacagcttta tccgcaaatt tccggtcata agagccgttt
     9841 tgccattcgt tttatgccgc tggacagtga aaacggacag gtaccggaac gtctggattt
     9901 cgaactggcc tgttgctaag gagtaaaaat gtcagaaact attacggtga attgcccaac
     9961 ctgcgggaaa acggtggtgt ggggtgaaat cagcccgttt cggccatttt gctccaaacg
    10021 ttgtcagctg atcgacctcg gagaatgggc tgctgaagaa aaacgaatcc ccagcagtgg
    10081 cgatctttcc gaaagcgatg actggagcga agagccaaag cagtgacatt ttatctgatg
    10141 cgacgctggc ctgataagac gcgggcagcg tcgcatcagg catcgtgtgc aaatgtcgga
    10201 tgcggcggga acgccttatc cgacctacaa gaccttacat atcaacatgt taaaattgta
    10261 gatactgata agacgcgcca gcgtcgcatc gggcatcttg tgcaaatgtt ggatgcggca
    10321 taaacgccta tccgacatac acacgcttaa gcttcgcaag taccggttca ttggctggcg
    10381 gaaaatcatc ggcattcaga ccgaccagcg acatccactc accgggttgc ccttctttac
    10441 cccacggctc cccttcccag ctttcgacca gccagaacca cagtgttata tgcctgtccg
    10501 ggaattcata ttccagtatt tcaaatagcg aaaaatgttg tggggtaatc ccgacttctt
    10561 cctgaagttc acgcaccacc gcctgttccg gcgtttcacc catttcaatt ttaccgccgg
    10621 gaaactccag tttattcgcc atgtgcgcat ctgcggcgcg acgcgttata aagattttat
    10681 tgttctcgtt gcgaataata cctaccgcaa tttgcagctt tttcattgtt ttaaacctat
    10741 aaaaaaggcg cagaatcctg cgccttttac ttcaattgtt agcttttatt gcaggcgacc
    10801 atggcactgc ttgtattttt taccagaacc gcacgggcaa ggatcattac gtccaacttt
    10861 gcgttcaccg gtttgtgccg ccagtgcagc tgcggctgca gagtcgtcat cctgatggct
    10921 aagctgctgc atttgcgcta aacgctcggc ttccatacga cgctgttgtt ccagctcttc
    10981 aacctcttca ggcatacgta cctggacttt gctcagcgtg ctgatgactt catatttcag
    11041 cgactccagc atcgctgcaa acatggagaa cgattcacgt ttgtattcct gcttcggatc
    11101 tttctgtgca tagccacgca ggtggatacc ttgacgcaga taatccatcg ccgccaggtg
    11161 ctctttccac agggagtcaa gcgtttgcag catgacgcct ttctcgaagt gacgcatcat
    11221 ctcagcgcca accacttctt ctttacgctg atacacttcg atggactgcg ccagaatacg
    11281 ctcacgcagc gtctcttcat gcagttctgg ctctttgtcc agccactcgg caatcggcaa
    11341 atcgaggtcg aaatcgttct tcagacgttc ctgcagcccc ggaatatccc acatttcttc
    11401 cagcgactgt ggcggaatgt aggcatcaat ggtcgctttg aacacatctt cacgaatgct
    11461 gttgatggtt tcactcacat cgctgacatc cagcagttcg ttacgctggg agtaaatggc
    11521 gcgacgctga tcgttagcca cgtcatcata ttccagcagt tgcttacgaa tgtcgaagtt
    11581 acggctttca actttacgct gggcgttggc aatcgctttg gtcacccacg ggtgctcgat
    11641 ggcttcgcct ggtttcatac ccagtttacg catcatgccg gaaacacggt cagaagcaaa
    11701 aatacgcatc agcgcatctt ccatcgacag gtagaaacgt gaagaaccgg catccccctg
    11761 gcgcccagaa cgaccgcgta gctggttatc gatacgacga gattcgtgac gttctgtacc
    11821 gatgatatgc agaccacctg cagccagtac cgcatcgtga cgcacctgcc agtcggcttt
    11881 gattttttca atttgctctg cggtcggatt ttccagcgcg gcaacttctg cctgccagct
    11941 accaccgagc acaatatctg taccacgacc cgccatgtta gtcgcgatag tcaccgccgc
    12001 cggataaccc gcttgtgcaa caatcgctgc ttcgttagcg tggaatttgg cgttcagaac
    12061 gttgtgtttg ataccggctt tggtcagctc gtttgacacc aattccgatt tttcgatgga
    12121 gatagtaccc accagcactg gctggccctt cgcagtacgc tctttgatat cttcgatgat
    12181 cgcctggatt ttttctgctt cagtcatata gaccagatct ggcagatctt tacgaatcat
    12241 cggacggttg gttggaacaa caacggtatc cagtttgtag attgagctaa attcgaaagc
    12301 ttcggtatca gcagtaccgg tcatgcccgc cagtttttca tacaaacgga agtagttctg
    12361 gaaggtgatc gacgccagcg tctggttttc gttctggatc tgtactcctt ctttcgcttc
    12421 cactgcctga tgcaggccat cggaccaacg acgtccctgc atggtacgac cggtgtgttc
    12481 gtcaacgatg ataacttcac catctttaac gatgtagtcg acgtctcggg taaacagcgc
    12541 atgagcgcgc agcgccgccg ttacgtggtg catcagcatg atgttggccg gagagtacag
    12601 agactcccct tcatccatga tgccttcttt caccagcagt tcttcaatca gcaccagacc
    12661 acgttcggtc aggttcacct ggcgagattt ttcgtccacc gagaagtggc cttcgccctg
    12721 gaaggtttcg gagtcttctt tttcctgacg gatcaggtgc ggaataattt tattcacgcg
    12781 tttatacatt tccgagctgt cttctgccgg gccggaaatg atcagcggtg tacgcgcttc
    12841 atcgatcagg atggagtcca cttcgtccac cagcgcatag tgcagtttac gctgtacacg
    12901 ttcttcaggg ctgaacgcca tgttatcgcg caggtagtca aagccgtatt cgttgttcgt
    12961 accgtaagtg atgtcagctg cgtaagcttc gcgctttgcc ggtgctggca tgcccggcag
    13021 gttgatgccg actgtcaggc cgaggaattc aaacaacgga cggttgtttt cggcgtcacg
    13081 ttgcgccagg taatcgttga cggtaacaac gtgtaccccc ttaccggtta atgcgttcag
    13141 gtaagcaggc agcgttgcgg tcagggtttt accttcaccg gtacgcattt ccgcgatgca
    13201 gcgttcgtta agaaccatac cgccgagtaa ctgaacgtcg aagtgacgca taccaaagac
    13261 gcgcttactt gcctcacgca ccacagcgaa agcttctggg atcagatttt ccagcacttc
    13321 gcctttttcc agacgtgcac gaaactctgc ggttttccct ttcagttctt cgtcggagag
    13381 tttttccatc tccggttcca tggcattgat gatgttgacg actttgcgca tccggcgcag
    13441 ggtgcgatcg ttacgactac cgaaaacttt agttaataat ttgattagca taataaaatc
    13501 tcaaacgccc cgcgttgcgg agtcaataaa atgaagtaaa ggtttattgt tgttagctga
    13561 ggcgttgagg gccagcacgg atgccttgcg cctggcttat ccagacgggc gtgctgaatt
    13621 ttgcttgcgg ggtaaaatgc gcataatcaa tgcgataacc cttttcagac ggcgtgcctt
    13681 cctgggtcaa cagcgcgctg agcgtatcca gtaatgcaag atgttgcgcc tgaagaggca
    13741 aagattcttc agcaacgggc agcgtttgcg gtgccattgc gaaggaaaga tgacggatta
    13801 ccgtgcgaat ggcatgttga tgccagtaat caacggaata gttcgaattc gggcggcgcg
    13861 tgttcgcttc cagcaaggcc aattgaccaa agttaacttt ggctgaaggc tcgtggttgc
    13921 gggttgtcgc ttttgcgggc gcgtttggtt cggcggcgtt gctgagcgca ggcaaaccta
    13981 aactcgccgc gaccatccct aataagagat gcggccagaa gtagcgttta ccaaactgtc
    14041 gccagcgcgt cagtattcca ctcacgttat tgccatcccg atcgtattca aacgtattta
    14101 gcgcacaaat cttatcatta aagcctgaag tccacagcac gaatgaagat aagttgtgcg
    14161 taaaaatgct taagaaaaca gcgttcgcac cgttttttat cgttcttata aaattcaaaa
    14221 aaaacgactg gttcatctgg ccggagagag tgccagattt gccagtcgaa ttttatacga
    14281 cagtataaat gtcgttatgc cagtacggct gaaggcgctt tgaaggccaa cggcagttct
    14341 gcgtcgtcct ggaaggtcac atattcccag gcttcctgtt ttgcaaggac agcctgcagc
    14401 agtttgttat tcagtgcatg accagattta taagcggtaa atgcaccaat aatattgtga
    14461 ccacacatga acaagtcacc gatcgcatcg agcattttgt gacgcacaaa ttcgtcttca
    14521 aaacgcaggc cgtcttcgtt cagtacgcga taatcgtcaa caacgatggc acaatcgaag
    14581 ctgccgccca ggcacaaacc acgggactgc agatattcga tatcacgcat gaaaccgaac
    14641 gtacgcgcac ggctgatctg gcgcataaac gcatcagcgg agaagttcat cgcatagcgc
    14701 tggttgctgg aatcaatagc cggatggtta aaatcgatgg tgaaatccag cgaaaaacca
    14761 ttgtacggct taaattcagc ccacttatcg ccatcttcga cacgaacagt ctctttgatg
    14821 cgaacaaatt tcttggcgca gttcaactcg tcgataccgg cgtcaagcag caggtataca
    14881 aacggagcgg cgctgccgtc catgatcggg atttccggcg cgttaacttc gataacaatg
    14941 ttatcgatgc ccaggcccgc gagagcagca ttgaggtgct ctacggttga aatccgtaca
    15001 tcatgctcgt tgaccagaca cgtacagagc atggtatcac gcacagattt ggcatcggcc
    15061 gggaaatcta ccggtggatt caagtcggtg cgacgataga tgaccccggt gttggccggc
    15121 gcagggcgta acgtcagggt gactttcttg ccggtatgta aaccgacacc cgtcgcctga
    15181 acgatacgtt taagtgtcct ttgtttgatc atcgtattat ctcgccaaat tacctatcca
    15241 accgaagtgt actatacatt cggcgggcca gtttagcaca aagagcctcg aaacccaaat
    15301 tccagtcaat tcttaatcag cttgcttacg caggaatgct gggatatcca gataatccgg
    15361 ctctttcgca gtttgcggtg cgttgtcatt cacgacttta gcaaccggtt tctgctcctg
    15421 agtcagcggt gccatcccgt gctgctggta gcgatccatc actggctgct gaacctgctt
    15481 attggtcacc agggtgattt caggacgttt gtccataccg atacctgtcg caacaacggt
    15541 tacgcgcagc tcgtcattca tatccgggtc aagagaagta ccgataacga cagttgcgtt
    15601 gtcggatgca aatgcacgga tggtgttacc taccgtttcg aactcatcca gacgcaggtc
    15661 gaagcccgcc gtgatgttaa ccagcacgcc gcgcgcgcca gacagatcga tatcttccag
    15721 cagcggagag gagatagcca tttcagcagc ttcttccgca cggtcttcgc cgctcgccac
    15781 accagaaccc atcattgcgt agcccatttc agacattacg gtacgtacgt ctgcaaagtc
    15841 cacgttcatc aggcctggac gagtaatcag ttcagcgata ccttgcacag cacctttcag
    15901 tacatcgttc gctgcgccaa atgcatccag cagggagata ccgcggccca agactttcag
    15961 cagtttgtcg ttagggatag tgatcagaga gtccacatgc ttggacagtt cagtgatccc
    16021 ctgctcagcg aatgccatac gcttcttgcc ttcaaagttg aaaggcttag tcacgacagc
    16081 aacggtcagg atccccaaat cttttgccac ttcagcgaca actggtgctg caccagtacc
    16141 ggtaccacca cccatacccg cagcaataaa gaccatgtct gcaccttcca gcgccgcacg
    16201 cagtgcatcg cgatcctcat cagccgcatt acggccaact tctggatttg cgccagctcc
    16261 cagtcctttg gtgataccgc taccgatttg aatcgtctgt ccaaccgctg ttttacgcag
    16321 cgcttgtgca tcggtattta ccgcgaagaa ttcaacacct tcaatgcgct cgcgcaccat
    16381 gtgttcaaca gcattaccgc cgccgccgcc gacgccgatg actttaatca ccgcgtcatt
    16441 ggtaagttcc attggttcaa acatagtttc tctccgattt gtgcctgtcg cctgaggccg
    16501 taatcatcgt cggcctcata aaaattaaaa ctcttttcgc agccaactat tgagtcgctt
    16561 gatccacgag ccaactgatg ctgtaacacg tttttctact tcagcttcac cgttaagatg
    16621 tgactctttc ccatagtgaa gcaatcccac cgccgtcgaa taatacggct cctgagcata
    16681 atccgttaaa ccggtaatgt tcagcggcgc gccgatacgc acttgcgtat gaaacacgcg
    16741 ctgagcacag gctgcaagac cttcgatctg cgctgcgcca ccagttaaaa caatgcctgc
    16801 cgccaggtga tgtttaaccc cttgttggcg aagcttttcc tgcaactgca atatctcttc
    16861 gttgaccagg ttgagcagct cggtatagcg cggctcgatc acctctgcca gggtctgacg
    16921 ttgcagactc cgcggtggac gaccgcctac gctcggcact tccacgctct catcttttcc
    16981 aacgatggaa cccagcgcac aaccgtggcg aactttaatc gcttcggcgt cgcttggcgg
    17041 cgtgccaaag gcgtaagcga tatcactggt cacgacattg ccagcataag gaattacctt
    17101 agtgtggcgc aatgccccac cggtataaac ggcgatatcc attgtaccac caccgatatc
    17161 gacgacgcag acacccagtt cacgttcatc ttccgtcaat accgaataac ttgatgccag
    17221 tccggcaaat atcagttggt caactttcaa cccacaacgt tcaaccgctt tgacgatgtt
    17281 tttcgccata tcgttgtgac atgtgatcag gtgcactttt gcctgcatcc gcacgcccga
    17341 aagtcctacc ggattcttga tcccttcctg atagtcaatc gcatactctt gcgggatcac
    17401 atgcagcaca cgatgctcat cgcgcacacg caccgatttc gcggtatgga cgacgttttc
    17461 cacatcttct tgcgtcactt cttcttcaga aataggcacc ataccaattt cattctggca
    17521 gctgatgtgc ttaccagaaa gcgccagata taccgaagag atctgacaat ctgccatcaa
    17581 ttctgcctgg tcaatggcgc gttgtacgca cttgaccacg gattcgaggt cgttcacccc
    17641 gcctttatcc ataccacgcg acgggcagct gcccacgcca atgatattga ccataccgtc
    17701 gggcagaact tcccctacta aagcggcaac cttcgcggta ccaatctcca gtcctactac
    17761 cagttttctg tccgtcgcct tgatcattgt tgttctgcct gtgcctgatt ttgttgctga
    17821 gtagattcct ctggcggcaa gggcgcccag cctactgccg ctccagagtc ataacgcaaa
    17881 tcaacgtagc taatccgttt gccatcggtt tgcgcctgct gctgtaaaac cggataaagt
    17941 tctacaaagc gagccaaacg tttcatcgta tcgccccggc caagattgag cttaatatcg
    18001 ttattcagcg tcaactgcca ggaacgccgc gcggtcatcg ccgcttcctt cagagtaaat
    18061 ctgtcctttg ccagcatctg ccccatttcg cgatagccct gcaacacttc attggcgctg
    18121 ccttccgggc catacagcat tggaagcacc tgcttgctgg tgcgatctgg cggcacgctg
    18181 aaagtgtttc cttccgcgtc taccatatgt tgatcattcc accgcgcaat cggcacatat
    18241 tcaaccagat gaatcttcaa ttcatcaggc cactgctttc tgacgctcac ctgcttaatc
    18301 cacggcaggc gttgttctat ttgcgtctgg atgatgttga catcctgggt cataaaggta
    18361 cctggctcac ccaatgccag gatcgactgc cggatatcgt cattacgcgt gtaatggcgt
    18421 tcaccggtca acaccagctt tgagagcggc aggcgttgcg catcttccat ccagcccaac
    18481 acgacccagc cgctcaccaa cactgtcgtt aaaacggtca gcaggaaaag gatccccgcc
    18541 agacgcgttc cattattgcg gcgagaagaa acctcttctt cgctgtttcg cgtgttcaga
    18601 gcagcctgcg acatattagt ccgccagttc cagaattcgt actaccaact gcgagaagct
    18661 catacctgcc tgacgtgccg ccatcggcac caagctgtgg ctggtcatac ccggtgaggt
    18721 attggcttcc agcagataaa actgtccatc gctgtccagc ataacgtcaa tacgtcccca
    18781 tcctttgcaa cctaacgtcg tccatgcttt cagcactaat gcctgcaaat tggcctcttg
    18841 tgacgcttcc agacctgcgg ggcagaaata ctgtgtctca tcagagagat acttcgcctc
    18901 ataatcatag aaggttccgg acggttgaat acgtattgac ggtaaaattt cttcaccgag
    18961 tatcgcaacc gtgaactccg gcccacttag ccatttttca atcaatactt cttcatcgtg
    19021 ctgaaatgcc aatcttaatg catcttgtag agcattttct gctactactt ttgacattcc
    19081 cacactggaa ccttcgcggc tcggcttaac gataaccggc aaacctaagg cagaaatttc
    19141 tgctaactgc ttatcgctca ggcctttttc aaactctgcg cgggttaatg ctacccacgg
    19201 cgctaccggt aaaccagcgc cctgccaaag caatttgctg cgtagtttat ccattgaaag
    19261 cgcagatgcc atcactccgc tgccggtata aggcaagccc atcagctcga gcataccctg
    19321 cagcgtgcca tcttcaccgc cgcgaccatg tagcgcgata aacactttct gaaagcccat
    19381 cgacttcagt tgcgtcacgt cgacttcttt cgggtcgaca ggatacgcgt caataccgcc
    19441 ttcacgcagc ccggctaaca ccgctgcacc agaattcaga gaaacttccc gctcagcgga
    19501 ggtcccgccc aacaggaccg cgattttatc agtcatgttg ttcttcctcc ggagtttgcg
    19561 gcttcagttt gatttcagct aaagaacggg caatttttcc gatattacca gccccctgaa
    19621 cgagaatcag gtcgttaccg gttaataccg gtgccagcat ctcggctacc tgcgccgggt
    19681 ccggcaccag aatgggatca attttcccgc gtccacgaat tgtgcgacac agcgaacggc
    19741 tgtccgctcc cggaattggc gcttcgccag ccggatacac ttccagcatc aacagggtat
    19801 caacctgcgt cagcacattg gcgaaatcat catacaggtc gcgcgtacgg gtaaaacggt
    19861 gcggctgaaa cagcattacc aggtttttat ccggccagcc tgcacgcgcc gctttaatgg
    19921 tggcgtccac ttccgtcggg tggtggccgt agtcatcgac cagcattgcc gtaccgctct
    19981 taccattcac tggctccagc gggaattcac cgaggaaatc aaaacggcga ccagtcccct
    20041 ggaagctctc cagcgcacgc aaaatagcct tgtcgtcaat tccctcttcc gtagcaaccg
    20101 caaccgcagc tgcggcgttc agcgcgttat gacggcctgg cgcattcagg gtgacgcgca
    20161 tcggctcttt gtcctggcgc agcagcgtaa agtgcccctg cgggccaatc tgctgataat
    20221 cttctacacg cacgtcggca tcttcgctga agccgtaagt cgtagtctga cgccccacac
    20281 gcggtaacaa ttcgcggatc accggatcat caacacacat caccgcacga ccgtaaaacg
    20341 gcaggttgtg cagaaaatta ataaaggtct gttttaaatt ctcaaagtcg ccctggtagg
    20401 tatccatgtg gtcggcttcg atattggtga caatcgccac catcggttgc aaatggagga
    20461 acgatgcatc gctctcatcc gcttcggcaa tcaggtatcg accatgcccc aaacgcgcat
    20521 gaacacccgc cgcttttacc agcccgccgt taacgaaggt tgggtcgagc cccgcttctg
    20581 cgtagatgct ggaaaccatc gcagtggttg tcgttttgcc gtgcgttccg gcaatggcga
    20641 tgccatgacg aaaacgcatt aactcagcca gcatttccgc gcgacgaatc accggaatac
    20701 gcgcttcatg agctgcgaca atttccgggt tatcggcaga aatcgcgctg gaaacaacga
    20761 ccacgctggc atcacgtacg ttttctgggc gatggttgaa ataaatcgtc gccccaagat
    20821 tcattaactg ctgcgtgacc ggatttggcg ctaaatcgga accactgatc tgataacctt
    20881 cattggccag aacttcggca ataccgccca taccggcacc accgatgccg acaaaatgta
    20941 tgtgccgaac gcgacgcatt tcgggcacga tggaacgcag ttttgccaat tgttgtgtat
    21001 tcattcttta cgccattaac ttcttaaatt catacgatgc aaaaggcatc gctacaatta
    21061 cgcccgggca gcccggctca cttcatttgc cactcgctcg gtggcatccg gaatggatgc
    21121 ggcgcgggcg cgttctgcca tggttaataa ggtttctcgc gaccacccgg ccagggtgtt
    21181 ggcgacagca tccacgctaa gctgtggctg ctcgataatt ttggctgcgc ccgctttttc
    21241 cagcggtagc gcattccagt attgctggcg gtctttatgt tgaaacggca caaacaacgc
    21301 cggtagtcct gccgcggcga tttcactcac cgttaacgca ccggagcggc aaacgacgac
    21361 atccgcccac gcatacgccg ccgccatatc atcaataaat tccgtcactt tatgctgcgg
    21421 ttgccccgct tcggcatacg cctgttcaac ggattgttgc gaacctttac cgctctgatg
    21481 ccagatagtg actgaatcac ccagtttcgc agcaacctgc ggcattgtct ggttaagaat
    21541 gcgtgcgccc tgggaaccac ccactaccaa cacacgaacc ggaccttcac gtccagccaa
    21601 acgttgctgc ggcaacggca gcgccagcac atcggtacgc accgggttac ccaccacttc
    21661 cgcattaggg aaagcgcctg gaaacgcctg catcactttg gtggcaatct tcgccagcca
    21721 tttattggtt aatcccgcaa taccgttttg ttcatgaagc acaaccggaa tacctaacga
    21781 ccacgcggcc agaccacctg gacctgatac gtagccgccc ataccgagca ccacgtcagg
    21841 tttgtacgct ttcataatcg cccgcgcctg acgccaggcg ttgaagatac gcagcggggc
    21901 agctatcagt gcttttatac cttttccacg cagaccagag atacgaatga aatcaatttc
    21961 gatgccatgt tttggcacta agtccgcttc catacggtcg gcagtcccca gccagcgaac
    22021 ttgccaaccc tgagccatta gatggtgcgc aaccgccagt cccgggaata catgtccacc
    22081 ggttccgcct gccatcacca ttaatcgctt tccttgacca ctcatcgtga acctcgtaca
    22141 aacgcctgcg ctttctccag acgcgtttca taatcaatac gcaacagcat catgatggct
    22201 gtcgacataa tcaataagct cgaaccaccg taactgatca gcggcaatgt cagacctttg
    22261 gtcggtaaca tccccgccgc cgcgcctacg ttaaccagcg cctggaaact aaaccagata
    22321 ccaatagaac aggcgagaaa accggaaaaa cggtggtcaa tttctaatgc tttgcggcca
    22381 atcgacatcg cgcgaaaagc gacgaagaat accattaaaa gtgccagcac cacaccgaca
    22441 taccccagtt cttcgccgat aatggcgaaa ataaagtcag tgtgcgcttc cggcagatac
    22501 tccagttttt gtaccgagtt acctaaacct tgtccccaca gttcaccgcg accaaacgcc
    22561 atcaacgatt gcgttaactg atagccgctg ccaaagggat cttcccacgg attccagaat
    22621 gcggtaacac ggcggatacg gtacggttcg gcgaggatca gcaacacaac cgctgaaatg
    22681 cccataccga taatggcaat gaactgccac aatttcgctc ccgccaggaa caacatcgcc
    22741 agcgtagtca caaacaacac caccaccgta ccaaggtctg gctgtgccag cagtaacact
    22801 gccaacacca gaattacgcc catcggtttc agaaagccgc gcaggttatt acgtacttcg
    22861 tcgcctttac gcaccagata gttggcgata tagcaaaaca gcgacagttt tgtcagctcc
    22921 gcaggctgaa tacgcagcaa accgagatcg atccaacgcg atgccccttt aaccgagcta
    22981 cccactacca gaacgatcat cagcaggatg atagatccga gcagcatcgt ggcactgtag
    23041 cgttgccaga actccatcgg cagacgcagc gtaatgatcg ccagaatgaa tgccaggatc
    23101 agatagacgc catcacgctt cgcgaagaag aacggatcgt tggttaagcg ttgccctatg
    23161 ggcattgacg ccgaggtcac catgataaag ccaatcgccg cgaggccgaa ggtcagccac
    23221 agtaaggtgc gatcgtacat gatcaggctg tcggtatctt tttcccgcga gcccatcacc
    23281 cagcccttta gcgccgtgga gatccagacc agaatactga atcctggcag gcgcggcatt
    23341 ttcaggcgag ggagagataa acgcatcaac ctaactcctt cgccagacgg gcaaactcat
    23401 tgcctcgttg ttcaaagttc ttgaactgat caaggctggc acaggccggg gagagcagaa
    23461 ccatatcgcc cggctgaaca cgcggagcca gcaagcgcat cgcctgttcc atggtttcgg
    23521 tttgttctgc cacttccggg cgtagcgccg ccagctgcgc gccgtcccga ccgaaacaat
    23581 acagacgtac gttatcgcca ttcaggtaac gcgccagtgg gctaaaatct gccgatttac
    23641 catcgccgcc cagcaacaaa tgcagcgtgc cgtctacgtg caggccattc agtgccgctt
    23701 ccgtactgcc gacgttggtc gcttttgaat cgttaatcca gcgcacaccg ttatgctcca
    23761 gcacaacttc aaagcgatgc ggcagaccag tgaatgtggt taatgctttc aggctgctgg
    23821 ctcgcggcaa accagcggca tctgccagtg ccagagctgc cagtgcattg gtgtagttat
    23881 gctgcccgga aagtttcatt tctttcacgt tcagcacttt ctcgcctttc acccgcagcc
    23941 aggtttcacc ctgctgatga ttcaggtgat agtcacccat gttgacgcca aagctgacgc
    24001 agcgttcatc cgcaccgcga atcggcattg ttaaggcatc atcagcatta accacgcaaa
    24061 tttttgcgtt ttcgtaaatg cgcagttttg ctgcacgata ctgttgtaaa ccaaacggat
    24121 agcgatccat atgatcttca gtcacgttca gaatggtcgc tgctaccgcc tgtaaactgg
    24181 aggtggtttc aagctggaag ctcgacagct ccagcacgta cagttcgcac tcatcatcca
    24241 gcagcatcaa cgcaggcagg ccaatattgc cacccacacc aacgttaacc cccgccgctt
    24301 tcgccatttc acccactagc gtggtgaccg tgcttttgcc gttagaaccg gtgatcgcca
    24361 caatcggtgc ttgtgcttca cgacagaaca gctcgatatc gccaacgatt tcgattccgg
    24421 catcagcggc agcgcttaag gatggatgcg ccagtgcaat accgggactg gcgacaatca
    24481 gatcggccgc catcagccat tcatcattca gaccgcccgt gtggcgttct acagcttcgg
    24541 gtaatttatc caggccaggc ggtgtcatac gcgtatccat aacgcgcggc gtcacaccgc
    24601 gagcgaggaa aaagtccacg caagaaagcc cggtgaggcc caggccgata atgacgacat
    24661 ttttaccctg gtaatcagcc atgattaacg taccttcagc gttgccagac caatcaggac
    24721 cagcatcagc gaaataatcc agaaacgtac aatgacgcgc ggttccggcc agcctttcag
    24781 ttcatagtgg tgatgaatcg gtgccatgcg gaaaatacgt tgtccgcgca gtttaaagga
    24841 gccgacctgc aggatgacag aaagcgtttc taccacgaac acgcccccca taatcaccag
    24901 caggaattcc tgacgtagca gtacggcgat aatgcctaac gcaccaccta acgccagcga
    24961 accgacatcg cccataaaga cctgcgccgg ataggtgtta aaccacagga agcccagtcc
    25021 tgccccgact atcgcggtac agacaataac cagttccccg gcgtgtcgca gatacggtat
    25081 gtgtaaatag ctggcaaagt tcatgttgcc ggtcgcccac gccaccagcg caaaaccacc
    25141 ggcgacaaat acggtcggca taattgccag accgtcaaga ccatcggtca ggtttaccgc
    25201 gttgccagta cccacaatga cgaagtaagc cagcagaatg tagaacagcc ccagttgcgg
    25261 catcacatct ttaaaaaatg ggaccaccag ctgcgttgcg ggcgtgtctt tgccaacaag
    25321 gtacagcgcg aaggcgacac ccagcgcaat gaccgacatc cagaaatact tccaacgagc
    25381 gatcaaccct ttggtgtctt tacgcactac tttgcgataa tcatcaacaa agccaataac
    25441 accgtaacct accagcacta ccaacacgca ccagacgtac ggattggacg ggtaagccca
    25501 cagcagtacg gagatcacaa tcgctgtcag gatcataatc ccgcccatgg tcggcgtacc
    25561 gcgcttgctg aagtgtgatt caggaccgtc gttacgcacc acctgaccaa aggaaagttt
    25621 ttgcaaatga gcaatcatgc gcgggcccat ccacaatgag atgaacagcg cagtcagcag
    25681 gctgacgatg gcgcgaaacg tcagatagga aaagacgtta aagccggaat aatatttgac
    25741 caaatgttcg gccagccaaa ctaacatgtc ccattctcct gtaaagcgcg tactacctct
    25801 tccatggcag cactacgtga acccttaact aaaatcgtaa ttacctgttg ctcagcaatc
    25861 agtgatttaa gacgcgcgat aagcgcagtt ttatcggaaa aatgttcgcc aacaccgctg
    25921 gcagtgctga tagcatggct ttgtttgccc acgcttaaca cgcggtcaat accagctgct
    25981 tttgccgctt cgcccacctg tatatggcag gcttcgcttt cagcgcccag ttccgccata
    26041 tcgcccacca ccagcacgcg gtagcccggc atttcagcca gtacctggac tgctgcggtc
    26101 attgaaccga cattggcgtt gtaggagtcg tcgagcagca actggttttc cgccagttgg
    26161 atggggaaca gacggcctgg aacagctttc agatttgcca gccccgcttt gatagcatca
    26221 agcgttgcgc ccacggacat ggagagcgca gcggctgcca gcgcattcgc aatattgtga
    26281 cgccccggca acggcagcag aacatccacg ctaccggttg gggtttgcag ggtaaattcc
    26341 gtaccgtgcg aagtcacatg gatgttggtg gcggtgaaat cgctgttggc ggcattaggt
    26401 gagaaacgcc acactttgcg tgagccaatt acgctttgcc agttcagcca gtcgttgttg
    26461 tcggcgttca taatggcgat accgttttcc ggcaggccgc taaagatttc acctttcgct
    26521 ttcgcgacac ccgcaagcga gccaaaacct tccagatgcg ctgccgccag gttattgacc
    26581 agcgccgctt ccgggcgagt cagactcaca gtccaggcaa tttcgccctg atggttcgcg
    26641 ccaagttcaa ttactgcgta atcgtactct ggcgttaagc gcaacagcgt catcggtaca
    26701 ccgatgtcgt tgttgagatt gcctgccgta taaagcgtgt tgccacactg gcttaaaatc
    26761 gctgccgtca tctctttaac ggaggttttg ccggaggaac ccgtcagagc aaccacgcgc
    26821 gccggaactt gctggcgaac ccatgcagcc agttcaccaa acgccagacg agtatccttg
    26881 acgattacct gcggcagatc gatatccagc ggacggttaa ccagtagtgc acccgcaccg
    26941 ccagctttcg cctggtcagc aaaatcatgg gcatcgaaac gttcgccttt cagggcaaca
    27001 aacaggcaac ccggcgtcag ttttcgtgtg tctgtggtta cagcatcaag ggtgatatct
    27061 gcccctttta gttcaccgtt gagaatatcg gtaagttggc taagggttac gctaatcatg
    27121 caatcacccc cagcagacgc gccaccgtga cgcgatcgga gtagtccaga cgctgattgc
    27181 caacaatctg gtaatcctca tgacctttgc ccgcgaccag taccacatca ttctctttag
    27241 cctgcataac ggcgcaagtc accgcttcag cacggccttc catcactttg gcatgtccgg
    27301 catctaacat tcccgccaga atatcgttga tgatggcacg cggttcttcg gtacgcgggt
    27361 tatcgtccgt caccaccgcc acgtcagcaa actcttcagc aattgcgccc atcagtggac
    27421 gtttgccttt atcacgatcg ccaccacagc caaagacgca ccacagcttg cccgcacagt
    27481 gcaaacgcgc cgcctgtaag gctttttcca gtgcatctgg cgtatgcgcg taatccacca
    27541 ctaccgtcgg tttgcctggc gcagtgaaca cttccatacg tccacaaacc ggttgcagac
    27601 gcgcggcggt ttttaacaga tcagccagcg ggtagcccag cgccagcagc gtcgccagcg
    27661 ccagcagcaa gttgctgacg ttaaaagcgc ccatcagatg gctttcaatt tcgccatcgc
    27721 cccagcttga gctaaagcga atcgtcgcac cgctgtcgtg atagttcact tctgtcgctt
    27781 tcaaccagcg tccgtgacag ttcggattga tatgatcttc cattgatacc gcaaccgcgt
    27841 ccggcagttt tgccagccag cggcggccca cttcatcgtc ggcgttaata atcgcctgac
    27901 cgcaatgatg ctcagaataa agcagccatt tcgcggcttc gtagtgttcc atatcaccat
    27961 gataatcaag gtgatcgcgg cttaagttgg taaagaccga cgccgcaaat ttcaatgccg
    28021 ccacacggtg ctgtaccagt ccgtgggagg aaacttccat tgcgcaaaaa gtcgcgccct
    28081 gatccaccag ccccgccagc tcatgctgaa catcgactgc cgaaccggtt gtattttctg
    28141 tcgggatcac tttccccagc aggccgttac caacggtgcc cattaccgcg ctggtttcgc
    28201 caagcagttg gctccactgc gccagcagct gggtcgtcgt ggttttgccg ttggtgcccg
    28261 ttacgcccac gagacgtaaa ttgtcagagg gttcatggta aaagcggccc gccagtgcag
    28321 ataaacgctc gttgagctgg ctgagataga tgaccggtac gccgtgcatt tcacggattt
    28381 caccgtcggt cgcctcatct ttcgcctctg caataatggc agccacacct tgcgctatcg
    28441 cctgcgggat atatcgacgc ccgtccgcct gatgacctac tacagctaca aagagatcgc
    28501 ccgccgcagc cacacggctg tcgagtgtca tctctcgcag tgctcgcgaa ggtgcgtctg
    28561 gcacccacgg agcaagaagg tcgcgcaaat tacgatctgc cacctgtccc ctcgccttga
    28621 ttaatcacaa attcattttt atcgcccgtt gtcagcgcat ccggttcgat gttcatggta
    28681 cgcaatacgc cgcccatgat ggcaccaaag accggcgcgg aaacggcgcc gccgtagtat
    28741 ttacccgcct gcggatcgtt gataacaaca accagcgcga agcgcggctg actcgcaggc
    28801 gcaacgcctg cggtataagc aatatattta ttgatgtagc gaccgtccgg tccgaccttt
    28861 ttcgcggtac cggttttaat ggcgatacga tagcctttaa tcgccgcctt cacgccgccg
    28921 ccgcccggta gcgccacgct ttccatcata tgcaccacgg tgcgaacaat ggattccggg
    28981 aagactcgtt caccgggaac cggggggtca actttggtaa tcgacagtgg gcgataaatg
    29041 ccgtagctgc cgatagttgc gtagactcgc gctaactgta acggtgttac cattagcccg
    29101 tagccgaaag agaaggtggc cctctctatg tcagaccacc gttgtttttg aggatataag
    29161 ccactgcgtt ctccgaccaa ccccaaattg gtcgcttttc ccagtccaaa acgtgagtaa
    29221 gtatctacta acgctgagga cggcatcgct aacgccagct tggaaacacc gacgttactc
    29281 gacttctgta ataccccggt cagggttaat tcgctgtagc gtgccacgtc tttgatttcg
    29341 tggccgttaa ttcgataagg aacggtattc agtaccgagt tttcccgcac tacgccacgt
    29401 tgcaacgcgg tcatcaccac catcggttta accgttgaac ccggttcaaa cacgtcagtg
    29461 atggtacggt tacgcatcgc ctctttcggc gtgccactca gattgtttgg gttgtatgac
    29521 gggctgttag ccatcgccag cacttcaccg gtgttgacat ccaccagcac ggcgctacca
    29581 gattcagctt tgttaaaggc caccgcgttg ttcagttcgc gataaaccag cgcctgcagg
    29641 cgttcatcaa tactcagcgc caggttgtgc gccgcctgac tgtcggtgga agagatatct
    29701 tcaattacgc gaccatagcg gtctttacgc acaatgcgct cacccggctg cccggtaagc
    29761 catttatcga aactcttctc gacgccctca atcccctgac tatcgacgtt agtaaagccg
    29821 atgaggtgag cggtcacttc gccggatgga taataacggc gagattcttc acgcagatga
    29881 atccccggca gtttcagttt tttgatgtac tctgccatgt cagggtttac ctgacgcgcc
    29941 agataaataa agcgcccttt cgggttggcg ttaatgcggg ctgaaagctg atccagcgga
    30001 atattgagtg cgttagccag cgccttccag cgatcaccga cgctgatacc gccagcgtca
    30061 tgcacttctt tcgggtcagc ccaaatcgct tttaccggca cgctcaccgc taacgggcga
    30121 ccagaacggt cagtaatcat gccgcgggag gtggaaactt gctgaacgcg aagagaacgc
    30181 atgtcgccct cttttaccag catatccggg gagataactt gtaaccacgc tacgcgtccg
    30241 agcagaaaag ccagcgccag gagaatacag ccgcataaca acgcaaaacg ccaactgata
    30301 aagttggcat gttcttcctg acgttttggt ttctgcgttt tcgccgctgc tttcatgcgt
    30361 cgcgtttatc cttatttttg cactacgata ttttcttgtg acggatcaac atgctgcatt
    30421 tgcagctttt ccgtggcgat cctttccacc cggctatggt cgccgagcgc attctcttca
    30481 aggatcaggt tgcgccattc aatgtctaaa gcatctcgct ccagcaccag ttgttcgcgc
    30541 tgagcggtca gtaaacgggt atggtgcgcc gtggttacca cggtcaccgc cgtcaaaata
    30601 atgcaaatga acaggcagag tggcagcttc ccaaatcgca aaagatcatc accgataaca
    30661 ccaggcaatg catggcgctc gtggcttccc atcgatccct taactttgct tagagcttct
    30721 gtcactctgc tgatcatgcg ttcgtcctct ctgcaatacg cagaactgaa ctacgggcac
    30781 gagggttctc agccacctct tcttcacccg gcattaactt gcctagtgct cgcagctgac
    30841 ggccacccag ttttttgagc tgctcttcag tcatcggtaa ccctgccgga acttgcggac
    30901 cgcggctgtt ttcacgcata aaacgtttca caatacggtc ttccagcgag tggaagctga
    30961 tgatcgaaag ccgcccaccc ggggccagca cgttgagcga gctttttagc gcctgctcta
    31021 tctcctccag ttcactgttt acccaaatgc gcaccgcctg gaaggtacgg gtcgcgggat
    31081 gtttaaactt atctttcacc ggcgttgcag cagccacgac ttccgccagt tctttggtgc
    31141 gggtcatcgg ctgttcgcgg ttacgctcga caatggcgcg ggcaatgcgt ttggcaaaac
    31201 gctcttcacc ataggttttc aatacccagg cgatatcggc ttcttctgcg gtttgtagcc
    31261 attcagcggc tgactgccca cgggttgggt ccatacgcat gtccagcgga ccatcgcgca
    31321 taaaggaaaa gccgcgttca gcatcatcaa gttgcggtga agagacgcca agatcgagga
    31381 gaatgccgtc gatcttgccg ataagatcgc gctcggcaac gtattcgccc agcgcggaga
    31441 aaggtccgtg gatgatggag aagcgcggat catcaatagt cttcgcaacg gcgatagcct
    31501 gcgggtcgcg atcgatcgcc agcaaacgcc cctcttcgcc aagctgcgag aggatcagac
    31561 gtgagtgacc accgcgacca aaagtcccat caatgtagat gccatcagga cggatattga
    31621 ggccattaac ggcttcatcc agcagcaccg tagtatgttt atagttttcc atcattttat
    31681 agagacaagt cctgcagtcg ctccgataag tctccagtag ccaactgctc tgcgtcgata
    31741 tcttccttga cctgttgatg ccaggttgtt tcatcccaca gctcaaactt gttgaactgt
    31801 ccaaccagca tcacttcttt tgtcagcccg gcatgttgcc gcagtactgg cgcgattaac
    31861 aatcgacctg cgccatccat ctgacattcg ctggcatgac ctaacagtag gcgctgcaca
    31921 cggcgctcaa ccgggttcat gctcgacaga cgcgataatt tttgctcgat aatttcccat
    31981 tcaggcaggg ggtaaagcag caggcacggg tgatgaatgt caatggtgca aaccatttga
    32041 ccggcagcgt tctcaagcag ctgttcccga taacgggtag gcactgataa gcgccctttg
    32101 ctgtcgagat tgactaacgt tgctccccgg aacatgccag cctcacccct tattcccact
    32161 ttgccccaca atttcccact gaaaggagtt tacggagcgg aggaaaagct tgtcaagcca
    32221 gtcacaaggc ataccgaggt aaaaaacccg atgtttacgg ctaatatcag cgttgtttat
    32281 tttacgtcca acgtgcgaag caaattaacg ttatgaatag ctgtaagaaa aaatccctca
    32341 tcgtcatcac cgttttaaaa tgactcacta ttttccacag caaatataaa gtgtcagttt
    32401 gcgacgcgag cggcatttta ggacatatct tccccggtta acagtccctg ttgcgtctgt
    32461 gtggcgcttg atgccagcgg gtttgcgcaa aagcaaagaa aagtgtttgt taaatcggca
    32521 attcatctct gcatgtaaca aaataatgca aaaacgcgct atcgatatca cttaattatt
    32581 gtgaatttca gctggtgagg taattaacgg gcaagtttaa aagcatttat caataatagc
    32641 caggaatagt cttatttact ttaaggatat tgatgtccat tcccgtactc aacgcgccag
    32701 agagaaattc acctggcgcg tatttttgtt cgcggcttag ctacggctga gcacgccgcg
    32761 gcgatagaga ttacgtttaa tgcgcgttaa accaggtttt ggcttacgcg gttcgtccag
    32821 gcttgccagg acaatctcca gcacacgctc tgcgacatcg cggtgacgtt gagccactgc
    32881 cagcaccggg cactgtaaga agtcgagcag ttcgttatcg ccaaaggtgg caattgccag
    32941 gtcagaaggc agtttgccgt cacgacgtag cgtgacatcc atcacccctt gcaacaacgc
    33001 aaacgacgtt gtgaacagtg cctgcggcat cggatgcgtt tccagccatt tttcgaataa
    33061 ctgggcagcc gcctcccgct catagctgtt ggcataaagg aaatgcactt cgcgcggatc
    33121 atctttccag gcagtacgga aaccttgttc acgcaggaag ctgacagaaa gctccggcag
    33181 cgcgccaaga taaagtaccg tctcggcggg aaacttacgt aactcttccg ccagcatttc
    33241 ggcatcatcc tgatcggcac caaccacgct ggtgaaatgt tcacgatcga gggcgcggtc
    33301 cagcgcgaca atcgggaacg ggtcgttagc ccagcgttga taaaaaggat gctcaggtgg
    33361 caacgacgtc gaaacaataa tggcatcaac ctgacgctgt aaaagatgct cgatacaacg
    33421 catttcgttg tcgggctgat cttccgagca ggcaatcagc agttgataac ccctttgccg
    33481 cgcctggcgt tcaagatagt tagcgatgcg ggtatagctg gtgttctcca gatcggggat
    33541 cacaagacca atagaacgtg tgcgtccagc acgaagccca gctgccacgg cgttcgggtg
    33601 gtaattgtgc tcacgcacca cagccatgac tttttcaacg gttttgtcgc tcacacggta
    33661 ttgcttcgct ttgccgttaa taacatagct tgcagtggtc cgcgacactc ccgccagccg
    33721 agcgatttca tccagtttca caattgcccc ttgcgtaaaa tgtaaaaacc atagccattg
    33781 tactggtatg ggttaaattc attaacatct aagcgcagaa aattaactgc ggcaactgct
    33841 tttattccag gttcccactg atttcgcaaa aaaagcccaa cgtgacatgt tgggctgcaa
    33901 attgcgcatt gagatcattc tcaacgcatt attttatcgc cgcgcgaaag tccgactaca
    33961 ccagagcgag caacttccac aattttcgcc acatcgcgaa tcgatgctaa aaatgcatca
    34021 agcttatcgc tggtacctgc taattgaacg gtataaagcg agggtgtgac atcaataatt
    34081 tgcccacgga atatttccgt attacgtttc acttcgtcac gcccgtagcc gctggcctga
    34141 attttcacca gcatgatttc ccgctcaaca tgcgcgccct gccctaactc gctcacgcgc
    34201 aagacatcga ccagcttgtg taattgcttt tcgatctgct caagcacttt ttcatcgccc
    34261 acggtctgga tggtcatacg cgataatgtc ggatcgtcgg ttggcgcaac ggtcaggctt
    34321 tcaatgttgt agccacgctg ggaaaaaagg ccaatcacgc gggataacgc gcctgattca
    34381 ttttcgagta agactgataa tatccggcgc ataatcaggt tctctccgtt ttgcttaacc
    34441 acatttcatc cattccgccc ccgcgaatct gcatcgggta gacgtgctca ctgccatcga
    34501 cggtaacatc aacaaacacc aggcgattat tgcgcacctg ttccagcgcc tcgctaagtt
    34561 tgctttccag ttcttgcgga tgagagatct ggatcccgac gtgaccatag gcttccgcca
    34621 gacgaacgaa atcgggtagt gactgcatat aagattgtga atgacggccg gaatagatca
    34681 tgtcttgcca ctgttttacc atccccagat agcggttatt gagattcacc accagtaccg
    34741 gcaactcgta ttgcaacgct gtagacagtt cctggatgtt catctgaata ctgccgtcgc
    34801 cagtgacgca aaccacggtt tcttctggca acgccatttt gacgcccagt gccgcaggta
    34861 aaccaaaacc catcgtgccg aggccgccag agttaatcca gcgacgcggt ttgtcgaatg
    34921 gataataaag cgcagcaaac atctggtgct gcccgacatc ggacgtcacg taagcgtccc
    34981 cattagtcaa ccgccaaaga gtctcgatca ccgcctgcgg tttaatcttt tcactgtgag
    35041 tgtcatattt caggcactga cgagcgcgcc actgttcaat ctgctgccac cagtcgcgga
    35101 tctcatccag cggttgatgg acggattctt gcgacaggag ttcaagcatt tgttcgagga
    35161 cctggcgagc atcccccaca atcgggatat cagcagtcac ggttttagaa atggaagtag
    35221 gatcaatatc gatatgcaga accgtggcat ttgggcagta cttcgccaga ttgttcgtcg
    35281 ttcggtcatc aaaacgtacc ccgacggcaa aaatcacatc cgcgttatgc atcgtcatat
    35341 tggcttcgta ggtgccgtgc attcccagca tgcccagtgc ctgacgatgc gttgccggaa
    35401 acgcccccag ccccattaat gaggaaacaa cgggcagatt caacgcctcc accgtttctt
    35461 ttagctgctg atgacagccc gccgtgattg ccccaccgcc tacatagaca accggttttt
    35521 ttgccgccac cagcgtttgc agagcgcgct taatttgccc tttatgcccg gaggtagtgg
    35581 gattgtaaga tcgcatactg accgactccg gccagacgta gggtaatttg ttcgccggat
    35641 taagaatatc tttgggtaaa tcaacgacca cgggcccagg gcgaccactt gccgccagcc
    35701 agaaagcctt tttcagcacc tgcggaatgt cttccgtttg cttaaccaga aaactgtgtt
    35761 taaccaccgg gcgagaaatc cccaccatgt cgcactcctg aaaggcatcg tagcctatca
    35821 acgaggtcgc tacctgcccg gaaaggacaa ctaagggaat ggaatccata taagcggtgg
    35881 caatgccagt aatcgcattg gtcgcccctg gacccgacgt taccagcacg acgccgactt
    35941 ccccggtcgc gcgcgccaga ccatcggcca tatgcaccgc cgcctgctca tgacgaacta
    36001 aaacatgatc gataccaccc acggtatgca atgcatcgta aatatcaagg accgcgcctc
    36061 cgggataacc gaatacttgt ttaacgccct gatcgataag cgatcggacg accatctcgg
    36121 ctccagacaa catctccatg gcctgcctca ctgtttgacg gaaaaaatgt gtaaaacaca
    36181 ttaaccgtcc gatcttgctt aagcaattgg cagcctgaga cataaacacg gggtaataag
    36241 aataaacagg aggaaaggtg aaaagaaacg ataaaatcct ccattacaga ggattaaatt
    36301 gataataaat cggctgaatc ccacaactta cagcaatttc tctatttttt taattaatcc
    36361 taaaatagcg agatcacaca ttcagaataa aattctacat tcaataaaaa atgcagaata
    36421 aaccagacat taatgtctga cctattctgc attcagttag cgtttgcaaa ttgagactaa
    36481 ttgctcttcc atccactgat ggcctttatc gcgcccggca gcttcatgcc aggagagata
    36541 acaggttctg ctgttttgtt ttaacggcag cggtaatacc tgtaactcta aggattcagc
    36601 gaactcttca gccagccaac gcggcgcaat agcgaccaga tgcgtttgcg acaccacgct
    36661 aagtacgctc atcattgcca tgccctgata cgcgatactg gcttgcttat ctaccgtgtc
    36721 ataccaggtt tgactaaatg acgcgaaacg atcgagcgaa accgcagcat gttgttcgtt
    36781 ataaacatca tgtttcagta acgggccttt aattgttgga tgatttttgc tggctaccaa
    36841 caccatttca tctttaaata atggcacact ggtaaattca gggcgatgga agtcttcata
    36901 actaatcaca aactccgttt cctgataacg cagctgatgt tcagtgttct gatttaatga
    36961 agacttgaac ataacatgta tatttggcgc aatctgctca atgtgattat aaatctgcga
    37021 ggtcagaatg ctgtctaacg ggctgcaaac acaaagatga aatacacgtt cactgctcgc
    37081 gggttcaaaa cctgaaccag gcaattcatt ttgtactagt tgcaatgcct gacgaactga
    37141 gccaaaaagt tgaaatgcgc gagcagtcgg ttgaatacca cggccataac gaacaaaaag
    37201 ctcgtcatta aacatcacct tcaggcgtgc aacagcgtta ctgaccgcag gttgcgacat
    37261 tcccagaaca tgagcggcac gggtgatgtt ttgctcctgc atcacggcat cgaaaacggt
    37321 taataagttg agatcgacca tgcgtagctg tggcttgctt aactccgccg tctctggatg
    37381 atctgtttga acctctggca tacttaactc cactgtcaca cttaactccc tttcccttat
    37441 tggaatgcag agaaataatc ctcgagaata tgatttatca tgcatataaa ataagaaaaa
    37501 ggaaaatgag taaaattagg aaaacataaa gatacgggtt ctgataaaaa cagaatccat
    37561 tgccgtggaa agcgtcattt accacagcat aataatccat aatgtttttg ttgcgaaaac
    37621 aatct
//
LOCUS       DAPHCV010000041        37606 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.041, whole genome shotgun sequence.
ACCESSION   DAPHCV010000041 DAPHCV010000000
VERSION     DAPHCV010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.041"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(112..864)
                     /gene="yecC"
                     /locus_tag="RH033_002024"
     CDS             complement(112..864)
                     /gene="yecC"
                     /locus_tag="RH033_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HDV2991212.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(861..1529)
                     /gene="tcyL"
                     /locus_tag="RH033_002025"
     CDS             complement(861..1529)
                     /gene="tcyL"
                     /locus_tag="RH033_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HDV2991213.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1544..2530)
                     /gene="dcyD"
                     /locus_tag="RH033_002026"
     CDS             complement(1544..2530)
                     /gene="dcyD"
                     /locus_tag="RH033_002026"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HDV2991214.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2635..3435)
                     /gene="tcyJ"
                     /locus_tag="RH033_002027"
     CDS             complement(2635..3435)
                     /gene="tcyJ"
                     /locus_tag="RH033_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV2991215.1"
                     /translation="MKLAHLGRQALMSVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3523..4074)
                     /gene="fliZ"
                     /locus_tag="RH033_002028"
     CDS             complement(3523..4074)
                     /gene="fliZ"
                     /locus_tag="RH033_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HDV2991216.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDALLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDPLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(4120..4839)
                     /gene="fliA"
                     /locus_tag="RH033_002029"
                     /pseudo
     CDS             complement(4120..4839)
                     /gene="fliA"
                     /locus_tag="RH033_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(5003..6052)
                     /locus_tag="RH033_002030"
     CDS             complement(5003..6052)
                     /locus_tag="RH033_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079755.1"
                     /note="structural flagella protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HDV2991217.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSTSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISLAQTAEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLSSIQDEIKSRLDEIDRVSGQTQFNGVNVLSKNDSMKIQIGANDNQTISIGL
                     QQIDSTTLNLKGFTVSGMADFSAAKLTAADGTAIAAADVKDAGGKQVNLLSYTDTASN
                     STKYAVVDSATGKYMEATVVITGTAAAVTVGAAEVAGAATADPLKALDAAIAKVDKFR
                     SSLGAVQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLS
                     KANQVPQQVLSLLQG"
     gene            6300..7715
                     /gene="fliD"
                     /locus_tag="RH033_002031"
     CDS             6300..7715
                     /gene="fliD"
                     /locus_tag="RH033_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HDV2991218.1"
                     /translation="MASISTLGVGSGLDLSSILDSLEAAEKSTLTPISKQQSSYTAKL
                     SAYGTLKSALESFQTANTALNKADLFTATSTTSSSSAFSATTTGSAIAGKYTISVSQL
                     AQAQTLTTKNSQKDSKAAIATSDSVLTIQQGGGKDPVTIDISAGNSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSITANDTGSNNAMKLSVSGDSALESFMGYNGTPGDSSNGM
                     IESVTAQNAKLTVNNVEIENSSNTISDSLEDITLNLNDVTTGNQTLTISKDTSKAENA
                     VKAWVDAYNTLQDTFSSLTKYTAVDAGAESQDSSNGALLGDSTLRTIQTQLKTLLANT
                     HSSSNYKTLAQIGITSDASTGKLEIATDKLQTALKNDAAGIGEMFIGDGKSTGVTTGI
                     SNNLTSWLSSTGIIQAAKDGVSKTLNNLTDQYNAASERIDTLMTRYKAQFTQLDVLMN
                     SLNSTSSYLTQQFDTSNSNSK"
     gene            7731..8141
                     /gene="fliS"
                     /locus_tag="RH033_002032"
     CDS             7731..8141
                     /gene="fliS"
                     /locus_tag="RH033_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDV2991219.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPD"
     gene            8141..8506
                     /gene="fliT"
                     /locus_tag="RH033_002033"
     CDS             8141..8506
                     /gene="fliT"
                     /locus_tag="RH033_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HDV2991220.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            8584..10071
                     /gene="amyA"
                     /locus_tag="RH033_002034"
     CDS             8584..10071
                     /gene="amyA"
                     /locus_tag="RH033_002034"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDV2991221.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(10105..10518)
                     /locus_tag="RH033_002035"
     CDS             complement(10105..10518)
                     /locus_tag="RH033_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV2991222.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            10705..11910
                     /gene="yedE"
                     /locus_tag="RH033_002036"
     CDS             10705..11910
                     /gene="yedE"
                     /locus_tag="RH033_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HDV2991223.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGLLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            11907..12140
                     /gene="yedF"
                     /locus_tag="RH033_002037"
     CDS             11907..12140
                     /gene="yedF"
                     /locus_tag="RH033_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDV2991224.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            12249..12746
                     /locus_tag="RH033_002038"
     CDS             12249..12746
                     /locus_tag="RH033_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000334561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HDV2991225.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSHMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQAVFIAAIGSTPFERGDEAGRSFCKPAQGLSVTVAMA
                     VRANL"
     gene            complement(12628..12957)
                     /locus_tag="RH033_002039"
     CDS             complement(12628..12957)
                     /locus_tag="RH033_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV2991226.1"
                     /translation="MKRLQAFKFRLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDPERAYKNFFRLRHHAQT
                     VCYLSRL"
     gene            12980..13057
                     /locus_tag="RH033_002040"
                     /pseudo
     CDS             12980..13057
                     /locus_tag="RH033_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557983.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            13116..13921
                     /locus_tag="RH033_002041"
                     /pseudo
     CDS             13116..13921
                     /locus_tag="RH033_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611130.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            13924..14376
                     /gene="tnpA"
                     /locus_tag="RH033_002042"
                     /pseudo
     CDS             13924..14376
                     /gene="tnpA"
                     /locus_tag="RH033_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407245.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(14533..15330)
                     /locus_tag="RH033_002043"
     CDS             complement(14533..15330)
                     /locus_tag="RH033_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV2991227.1"
                     /translation="MLAKDKTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQKFYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
     gene            complement(15340..15891)
                     /gene="ybcL"
                     /locus_tag="RH033_002044"
     CDS             complement(15340..15891)
                     /gene="ybcL"
                     /locus_tag="RH033_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HDV2991228.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(16060..16392)
                     /gene="emrE"
                     /locus_tag="RH033_002045"
     CDS             complement(16060..16392)
                     /gene="emrE"
                     /locus_tag="RH033_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HDV2991229.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(16736..17050)
                     /gene="fliE"
                     /locus_tag="RH033_002046"
     CDS             complement(16736..17050)
                     /gene="fliE"
                     /locus_tag="RH033_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HDV2991230.1"
                     /translation="MSAIQGIEGVISQLQTTAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            17265..18923
                     /gene="fliF"
                     /locus_tag="RH033_002047"
     CDS             17265..18923
                     /gene="fliF"
                     /locus_tag="RH033_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HDV2991231.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVTGSAAVAVMVALILWAK
                     TPDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDKGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEALTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREISDNDPRVVALVIRQWINNDHE"
     gene            18916..19911
                     /gene="fliG"
                     /locus_tag="RH033_002048"
     CDS             18916..19911
                     /gene="fliG"
                     /locus_tag="RH033_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HDV2991232.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            19904..20590
                     /gene="fliH"
                     /locus_tag="RH033_002049"
     CDS             19904..20590
                     /gene="fliH"
                     /locus_tag="RH033_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HDV2991233.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLARGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            20590..21963
                     /gene="fliI"
                     /locus_tag="RH033_002050"
     CDS             20590..21963
                     /gene="fliI"
                     /locus_tag="RH033_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HDV2991234.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            21982..22425
                     /gene="fliJ"
                     /locus_tag="RH033_002051"
     CDS             21982..22425
                     /gene="fliJ"
                     /locus_tag="RH033_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HDV2991235.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            22422..23549
                     /gene="fliK"
                     /locus_tag="RH033_002052"
     CDS             22422..23549
                     /gene="fliK"
                     /locus_tag="RH033_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HDV2991236.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSEILADAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMTLAAVAGNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQVRVTGNSGVDIFA"
     gene            23654..24118
                     /gene="fliL"
                     /locus_tag="RH033_002053"
     CDS             23654..24118
                     /gene="fliL"
                     /locus_tag="RH033_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HDV2991237.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            24123..25127
                     /gene="fliM"
                     /locus_tag="RH033_002054"
     CDS             24123..25127
                     /gene="fliM"
                     /locus_tag="RH033_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HDV2991238.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            25124..25537
                     /gene="fliN"
                     /locus_tag="RH033_002055"
     CDS             25124..25537
                     /gene="fliN"
                     /locus_tag="RH033_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HDV2991239.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            25540..25905
                     /gene="fliO"
                     /locus_tag="RH033_002056"
     CDS             25540..25905
                     /gene="fliO"
                     /locus_tag="RH033_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HDV2991240.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALITIIALILAAAWLIKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVIAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            25905..26642
                     /gene="fliP"
                     /locus_tag="RH033_002057"
     CDS             25905..26642
                     /gene="fliP"
                     /locus_tag="RH033_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HDV2991241.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            26652..26921
                     /gene="fliQ"
                     /locus_tag="RH033_002058"
     CDS             26652..26921
                     /gene="fliQ"
                     /locus_tag="RH033_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HDV2991242.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            26930..27715
                     /gene="fliR"
                     /locus_tag="RH033_002059"
     CDS             26930..27715
                     /gene="fliR"
                     /locus_tag="RH033_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HDV2991243.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            28005..28628
                     /gene="rcsA"
                     /locus_tag="RH033_002060"
     CDS             28005..28628
                     /gene="rcsA"
                     /locus_tag="RH033_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HDV2991244.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(28672..28860)
                     /gene="dsrB"
                     /locus_tag="RH033_002061"
     CDS             complement(28672..28860)
                     /gene="dsrB"
                     /locus_tag="RH033_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HDV2991245.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            29023..29250
                     /gene="yodD"
                     /locus_tag="RH033_002062"
     CDS             29023..29250
                     /gene="yodD"
                     /locus_tag="RH033_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HDV2991246.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            29549..30364
                     /locus_tag="RH033_002063"
     CDS             29549..30364
                     /locus_tag="RH033_002063"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HDV2991247.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLTRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQEIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(30361..32055)
                     /gene="yedQ"
                     /locus_tag="RH033_002064"
                     /pseudo
     CDS             complement(30361..32055)
                     /gene="yedQ"
                     /locus_tag="RH033_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(32226..32408)
                     /locus_tag="RH033_002065"
     CDS             complement(32226..32408)
                     /locus_tag="RH033_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HDV2991248.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(32487..33404)
                     /locus_tag="RH033_002066"
     CDS             complement(32487..33404)
                     /locus_tag="RH033_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HDV2991249.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            33577..34497
                     /gene="yedA"
                     /locus_tag="RH033_002067"
     CDS             33577..34497
                     /gene="yedA"
                     /locus_tag="RH033_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HDV2991250.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAAVIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(34486..34956)
                     /gene="vsr"
                     /locus_tag="RH033_002068"
     CDS             complement(34486..34956)
                     /gene="vsr"
                     /locus_tag="RH033_002068"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HDV2991251.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALIERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(34937..36355)
                     /gene="dcm"
                     /locus_tag="RH033_002069"
     CDS             complement(34937..36355)
                     /gene="dcm"
                     /locus_tag="RH033_002069"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HDV2991252.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISGCFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(36422..37117)
                     /locus_tag="RH033_002070"
     CDS             complement(36422..37117)
                     /locus_tag="RH033_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HDV2991253.1"
                     /translation="MDLQHWQTQFENWLKNHHQHQDAAHDVYHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     YRPLDDKRYALDHFQTKLLKLPQTMQTVRGKQLAQHNAQFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(37157..37522)
                     /locus_tag="RH033_002071"
                     /pseudo
     CDS             complement(37157..37522)
                     /locus_tag="RH033_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
BASE COUNT         9281 a         9739 c         9461 g         9125 t
ORIGIN      
        1 tcgcattctc ttatgttatt aatgagttat gctgatttgt taagctgttt tatcacgctt
       61 gaaatggcgt cccgccccga caggtgaatc gtcggggctg attttttctt attattgcag
      121 cagaaacttc tcgaggaact ggcgggtgcg aggctgctgg ggatcggcaa ataacgcttt
      181 tgctgccccc tgctcgacta tccgcccctg gtccataaaa atcgcccggt ccgcaacatc
      241 ccgggcaaag ctcatttcgt gcgtcacaat caccatcgtg cgcttttcct gcgccagctg
      301 acggatggtg ttcaggactt cgcccaccag ctctggatcc agcgccgacg ttggctcgtc
      361 aaacaaaatc acctcaggac gcattgccag cgcacgtgca atcgcaacac gttgctgttg
      421 accgccagac aaacgacgtg gatagctggt ttctttaccg gccagcccaa ctttcgccaa
      481 cagctcgcga gcgcgcgccg tggcctcttc tttcggttcg cctttgacga tcaccggccc
      541 ttcaataatg ttttccagca ccgtacgatg cggaaacaaa ttaaagttct ggaagacaaa
      601 cccgacgtgc tgacgcaact ggcgaatcag cgatttttgc tgacttaatg aacgtgcagt
      661 atcaatagtg atatcgccaa cggtgatagt tcccgcttcc ggttgttcca gcagatttat
      721 gctgcgtagc aacgtggttt tgccggaacc actcggaccg ataattgcca ccacttcgcc
      781 aggctttacc tcaaggtcga taccgtgcag caccgtctga ccgtgaaatt ttttcaccag
      841 gttcttaact tcaatggcac tcattttggt tctctctcct ggcgattaag ttgattctca
      901 aaatggttct gcaacgtcga taatactgtc gccatgatcc agtagatgag cgaagccgcc
      961 aggtacatgg tgaacacctc cagcgtgcgc gaggtaatca actgcgcctg acggaacagc
     1021 tccggcacct gaattgtcgc ggccagcgac gtatctttta ccaggctgat aaagctgttc
     1081 gacagcggcg gcaacgccac ccgcgccgcc tgtggcaaaa tcgcgcgacg catagtctgc
     1141 catggcgtca taccaatact ggcagctgct tcccactgac ctttatcaat agaagaaatg
     1201 gcggcgcgca gcgtttcggc agcataggcg gcagtgttga gcgacagacc aatcattgct
     1261 gacggaatgg gatctaattc gataccaaac tgcggcaggc cgtaatagat cataaacagt
     1321 tgtgcgatga gcggtgtacc acgaaaaata gagatataaa aacgcgccag ccagcgcacg
     1381 ggccagattg gcgacaggcg catcagcgcg agaataaacc cgagcagtaa gccaaaaaac
     1441 atgccgccga tactgagttg cagcgtatac ccagcccctt tcagcaggaa cggcaaggaa
     1501 tcaataacca gttgtatact ttcttgcatg agcgttcccg gagctaaacg tggggatgat
     1561 aggcgaacag cgcaggcgca ccgccggtat gaataaacag aatcggccct tcatctttga
     1621 aacgtttctg actgataccg tcaatcagac ccgccatcgc ttttccggta tacacaggat
     1681 caagcagaat gccttccagc cgcgccagca atttcactgc ttccatgcct tcgtcgttcg
     1741 gcacgccgta gccaggtgca aaatagtcat cccagagtaa aatttccgct gatgcggtca
     1801 gctccagttc tttcgcaatc gcctgttgta ggttaaccac tttcggcaat tgatcggcaa
     1861 cggaacgcga cacggtcacg ccaatcagtt cgctttcagg catcaggtgt tccagcccaa
     1921 cagccagtcc ggcgtgagtt ccggcactgc ccgatgcgac caccaccgac gaaatattaa
     1981 ccgccccttc acactgttgc gcgatttcca gcgcactctc cacataaccc agcgcgccca
     2041 gagcattaga accgccaacc ggaatgacat acgggcgaaa gccttgtgct tcgactcgcg
     2101 tcgccagctc ttccagttgg gcattgggat cggtcagtgc gtcgcacatt tcaatctggg
     2161 tattgaacag atccagcaac aagcgattgc cgttggttaa atagttttct gcggttgtgc
     2221 caataggatt ttccagcagc gccacgcagt gcagaccgag tttggcagcg actgcggcag
     2281 tctggcgcac atggttagac tggatcgccc cggcagtaat cagcgtatcg gcaccttcgc
     2341 gcagagcatc tgccgcgaga aattctagct tacgtaattt attgccgccc attgccatgg
     2401 gggtgacgtc atcccgtttg atgaaaattt cccgtcctag ataatcagaa aagcgcggca
     2461 gatattcgag cggcgttggc gcgccgataa actccaaccg tggaaaacgg gttaaattat
     2521 gcagtggcat aacagcctcc gatgtgtgtt gttgtgattt tcttattatg cacgctgaaa
     2581 acgcgtaaat aaaaaaggcg ctggtgaaag cgcccttttt tgtcattatg ctgattattt
     2641 ggtcacatca gcaccaaacc atttttcgga aagggcttgc agagtgccat ctttttgcat
     2701 ttccgcaatt gcatcgttca ctgctttcag caggtcttca tttcctttac gcagcgccac
     2761 gccagactcc tgacgggaga atgcttcacc ggttactgcc agcgtatcgt tggttttctt
     2821 caccagatcc agcgccgcca gacggtcaac gaggatcgca tcgatacggc ctacgcgcag
     2881 atcctgatat ttggtcgggt catcatcata ggtacgcaca tcgacgccct gaacattctg
     2941 ccgcagccac tcttcatagt tggtgcccag accgacaccc acttttttgc ctttcagatc
     3001 agcagccgtt ttaatggtgc cttcgttacc ttttttcacc agcgcctgaa taccagaaat
     3061 ggtgtacggg gtagagaaat cgtatttttt cttgcgctca tcagaaatgg tgacctgatt
     3121 aatcaccaca tcaatgcgtt tagagtccag cgacgccagc ataccgtccc atttggtcgg
     3181 ttttagtgac gcctcaacgc caagatgttt tgccagctgt tgggcaaatt ccacttcaaa
     3241 accggttaat ttgccgtcat ccccctgaaa actgaacggc ggataagtgc cttccagccc
     3301 taccagcagc gtgccgcgct ctttaacttt attaagcaga ccttcatctg caaaactttt
     3361 aacgctcatg cccgcaacca gcgctacggc catcacactc atcaatgcct gacgtcccag
     3421 atgtgctaat ttcatattta ccccgaatgt tgttatgtct gtttgcagtg tagagccatt
     3481 gtttgtaaac acaaaaacaa ctccgctaca tcttattctt atttaatata tatcagaagc
     3541 aggcaggctg gaggatttct gcacaagtcc ggtacacgtt tgacgctgat agtgttgata
     3601 cttccgtaat gcgatgcggt aattgttggt gctggtggtg ggcagccacg gggcgaggat
     3661 ttcatcaagg aaaccgtcct gcagcggatc gagggaaata ttttgctcgt gtagatgatt
     3721 ccccaaacgg cgcaaacgaa cgacatattc acgcaccgta cctgggctca tttcagtttg
     3781 ctcaaaaaga aattgcttaa agccgataat atcgaaaaaa tcactctgcg ttttgcaatg
     3841 taaatcaccg caaaatcggc acaatgccgc ccatgatttt tgttccgttt gccagccatt
     3901 ttcatcaagc agcgcgtcca ggcgggaaat ctcgatttta ttcactattt tgccgtcgcg
     3961 aacgagggta atgcgatcga gtaatttacg gcaatgcgcg caatgagtct ggctgtgttt
     4021 aaagtcttta agatagcggc ttaatggccg tcttttcagg tgctgcacca tcattaagaa
     4081 ctcctggtag tcaaagttaa agtgcggcat ttactgacgt tataacttac ccagtttagt
     4141 gcgtaaccgt ttaatagcct ggctgtgtaa ctgactgacc cgcgattccc cgacctccag
     4201 caccgcgcca atctctttga gattcagctc ttcctggtaa tagagggtta ataccagttt
     4261 ttcgcgctcc ggcaacgttt cgatggcttc catcacccgc tgacgcagat tactgtccag
     4321 cagttgttgt agcgggtttt ctcgctgatg atcatcagta accagttcga tgctatcgcc
     4381 gtgctcttcg cgccactcat cgtaggagaa gagctggctg ttattggtgt cgagcaacat
     4441 ttggcgataa tcggcaatat cgatccctaa acgttccgct acctcagttt ccgtggcgtt
     4501 gcggccaagt tcctgctcca gttgccctat tgcctgtgcc acttcacgcg cgttgcgtca
     4561 cacgctgcgc ggcacccagt cacggctgcg aagttcatcc agcatagcgc cacggatacg
     4621 ctgcactgcg taagttgtaa atgccgttcc ttgtagggcg tcatagcgtt cgacggcatt
     4681 aagtaaccca atgccgcccg cctgtagcag atcgtcaagt tccacgctcg cgggcagtcg
     4741 aacctgcagg cgcaatgctt cgtgacgcac cagcgggaca taacgctgcc acagcgagtg
     4801 tttatccatt acaccttcag cggtatagag tgaattcacg ataaacagcc ctgcgttata
     4861 tgagttatcg gcatgattat ccgtttctgc agggttttta atcggacgat tagtgggtga
     4921 aatgaggggt tatttggggg ttaccggtaa attgcgggca gaaaaaaccc cgccggtagc
     4981 ggggtcaggc aggttaaggc cattagcctt gtaacagaga cagaacttgc tgcggtacct
     5041 ggttggcttt agacagcacg gagttgcccg cctgctggat aatctgcgct ttcgacatgt
     5101 tggacacttc ggtcgcatag tcggcgtcct gaatacggga ctgcgcttca gacaggttgg
     5161 tggtggtgtt gttcaggttg gtgaccgcag aatccagacg gttttgaacg gcaccgaggg
     5221 aggagcggaa tttgtcgact ttagcgattg cggcatccag tgcttttaac ggatcggctg
     5281 tagcggctcc cgccacttcc gctgcaccaa cagttaccgc cgccgccgta ccggtaatga
     5341 ctacagtggc ttccatgtat ttaccggttg cagaatcaac gaccgcatat ttagtactgt
     5401 tagacgcggt gtcagtgtaa gacagtaaat tgacttgttt acccccagca tccttgacat
     5461 ccgcagcagc aattgctgta ccatcagcag ccgtcagttt cgccgcgctg aaatccgcca
     5521 tgccggacac ggtaaatcct ttcagattca aagtggtact gtcgatttgt tgcaagccaa
     5581 tgctgatcgt ctggttatca ttggcaccaa tctgaatctt catcgaatcg tttttcgaca
     5641 gcacgttcac accgttgaac tgggtctgac cagatacacg gtcaatttca tcaagacggg
     5701 atttgatttc gtcctgaata gaagacaggt cggaatcaga gttcgtgccg gtagaggcct
     5761 gaacggtcag ttcacgaata cgctgcaagt tgttgttaat ctctgacagc gcgccttcag
     5821 ccgtctgcgc cagagaaata ccgtcgttgg cgttacgtgc ggcctgagtc agacctttga
     5881 tgttagaagt aaagcggtta gcaatcgcct ggcccgcggc gtcatcttta gcgctgttaa
     5941 tacgcagacc agaagagagg cgctcgatag aagtcgacag cgcagactgg tttttgttga
     6001 tgttgttctg agtgatcagc gagaggctgt tggtattaat gacttgtgcc ataattcatt
     6061 ttcctgtttt caagtctacg gtttaagtat tgggcttcca cccttcggct tcgtcgccgt
     6121 caaccctgtt atcgtctggt cactcacaac ctttagaatt tttttctagc ggtcgaaata
     6181 ctccccttcc tcgttgctgt tcgtgccatt aaggcaaaaa ataaatcgta aactttaccc
     6241 cgcttacgcc gataaccccg gtattcgttc tatacacgtc gcatgattaa ggaaactata
     6301 tggctagtat ttcaacgctt ggggttgggt cggggctgga cttaagcagc atccttgaca
     6361 gtctggaggc ggcagaaaaa tctaccctga cgccgatatc aaaacagcaa agctcttaca
     6421 ccgcgaaact gagcgcctac gggacgctga aaagtgcact agagagtttc cagacagcca
     6481 acacagcact gaataaagcc gatttattta ccgccacctc aaccaccagc agttcatcgg
     6541 ctttcagcgc caccaccacg ggcagcgcga ttgctgggaa atacaccatt agcgttagcc
     6601 aactggcgca ggcacagacg ctgacaacaa aaaatagtca aaaagacagt aaagccgcca
     6661 tcgccaccag cgatagcgta ctgaccatcc aacaaggcgg cggtaaagat ccggtcacga
     6721 ttgatatcag cgcgggtaac tcgtcacttt ccggcattcg cgatgccatt aacaacgcca
     6781 aagctggtgt cagcgccagt atcatcaatg tgggaaacgg cgaatatcgt ctgtccatca
     6841 ccgctaacga tacgggcagc aataacgcta tgaaacttag cgtcagcggc gacagcgccc
     6901 tggaaagttt tatgggttac aacggaacgc cgggtgatag cagcaatggc atgattgaaa
     6961 gcgtcaccgc gcaaaacgcc aaactgacag tcaataacgt tgagattgaa aacagcagta
     7021 acactatcag cgactcactg gaagatatca ccctcaacct gaatgacgtc accaccggta
     7081 atcagacact gaccatcagt aaagacacat ccaaagctga aaatgcggtt aaagcgtggg
     7141 tggatgccta taacacattg caggatacat tcagcagctt aaccaaatac accgctgtcg
     7201 atgccggagc tgaaagtcag gactccagta atggcgcatt actcggtgac tccacactgc
     7261 gtaccatcca gactcagctt aaaaccttgc tcgccaatac tcacagcagt tcaaactata
     7321 aaacgctggc acagattggc attaccagcg atgccagtac cggcaagctg gaaatcgcga
     7381 ccgacaaact gcaaacggcg ctaaaaaatg atgccgctgg tatcggtgaa atgtttatag
     7441 gtgacggcaa aagtactggg gtaaccaccg gcattagtaa caacctgacc agttggctct
     7501 cttccaccgg gataatccag gcagcaaaag atggcgtcag caaaacgctg aataatttga
     7561 ccgaccagta caacgcagcc agtgaacgta tcgatacctt aatgacgcgc tataaagccc
     7621 agttcaccca attagatgtg ttaatgaact cgctgaactc cactagtagc tatctgacac
     7681 agcagttcga cacctcaaat agcaactcga aataatcttc aggagagact atgtacgcgg
     7741 caaaaggcac ccaggcctat gcacaaattg gcgtcgaaag cgccgtaatg agcgccagcc
     7801 agcaacagct ggtcaccatg ctatttgatg gagtgctgag cgcactggtt agagcgcgcc
     7861 tgtttatgca ggacaacaat cagcaaggca aaggcgtctc tttgtcaaaa gcgatcaaca
     7921 tcattgagaa cgggctgcgg gtgagtcttg atgaagagag caaagacgaa ctaacccaaa
     7981 acttgattgc tctttatagc tatatggtca ggcgcttgct gcaagccaat ttacgcaacg
     8041 atgtctccgc agtcgaagaa gtggaagcat taatgcgcaa tattgccgat gcctggaaag
     8101 agtctttact ctccccttct ttgattcagg acccagactg atgaacaatg caccgcattt
     8161 gtatttcgcc tggcaacaac tcgtcgaaaa aagtcagctc atgttacgcc tggcgacaga
     8221 agaacaatgg gacgaactca tcgccagcga aatggcgtat gtgaatgcgg tgcaggagat
     8281 cgcacacctg actgaagagg ttgccccgtc caccacgatg caggagcagc tccgcccgat
     8341 gctgcgcctg attctcgaca acgaaagcaa ggtaaagcag ttattacaga ttcggatgga
     8401 tgaactggcg aaactggtcg gtcagtcatc ggtgcaaaaa tcggtgttaa gtgcctatgg
     8461 cgatcagggt ggctttgtgc tggctccgca ggataacctc ttttgaatct taaagagcca
     8521 atggctcgcg aataatctga ttacggctac gcttctaatg ttccccttga atggagtcga
     8581 agaatgcgta atcccacgct gttacaatgt tttcactggt attacccgga aggcggtaag
     8641 ctctggcctg aactggccga gcgcgccgac ggttttaatg atattggtat caatatggtc
     8701 tggttgccgc ccgcctataa aggcgcatcg ggcgggtact cggtcggcta cgactcctat
     8761 gatttatttg atttaggcga gtttgatcag aaaggcagca tccctactaa atatggcgat
     8821 aaagcacaac tgctggccgc cattgacgcc ctgaaacgta atgacattgc ggtgctgctg
     8881 gacgtggtag tcaaccacaa aatgggcgcg gatgaaaaag aagctattcg cgtgcaacgt
     8941 gtaaatgctg atgaccgtac gcaaattgac gaagaaatca ttgagtgcga aggctggacg
     9001 cgttacacct tccccgcgcg tgccgggcaa tactcgcagt ttatctggga tttcaaatgt
     9061 tttagcggta tcgaccatat cgaaaaccct gatgaagatg gcatttttaa aattgttaac
     9121 gactataccg gagaaggctg gaacgatcag gttgatgatg aactcggtaa tttcgattat
     9181 ctgatgggtg agaatatcga ttttcgcaat catgccgtga cggaagagat taaatactgg
     9241 gcgcgctggg tgatggaaca aacgcaatgc gacggctttc gtcttgatgc ggtcaaacat
     9301 attccggcat ggttttataa agagtggatc gaacacgtac aggaagttgc gccaaagccg
     9361 ctgtttattg tggctgaata ctggtcgcat gaagtcgata aactgcaaac gtatattgat
     9421 caggtggaag gcaaaaccat gctgtttgat gcgccgctgc agatgaaatt ccatgaagca
     9481 tcgcgcatgg gtcgcgacta cgacatgacg cagatcttca ctgggacgct ggtggaagcc
     9541 gatccttttc acgccgtaac cctcgttgcc aaccacgaca cccaaccgtt gcaagccctc
     9601 gaagcaccgg tcgaaccgtg gtttaaaccg ctggcgtatg ccttaatttt gttgcgggaa
     9661 aatggcgttc cttcggtgtt ctatccggac ctctacggtg cacattacga agatgtcggt
     9721 ggtgacgggc aaacctatcc gatagatatg ccaataatcg aacagcttga tgagttaatt
     9781 ctcgcccgtc agcgtttcgc ccacggtgta cagacgttat ttttcgacca tccgaactgc
     9841 attgccttta gccgcagtgg caccgacgaa tatcccggct gcgtggtggt catgtcgaac
     9901 ggggatgatg gcgaaaaaac cattcatctg ggagagaatt acggcaataa aacctggcgt
     9961 gattttctgg ggaaccggca agagagcgta gtgaccgacg aaaacggcga agcgaccttc
    10021 ttttgcaacg gcggcagcgt cagcgtgtgg gttatcgaag aggtgattta aattcatccc
    10081 cggcggcaag ccggggagat ttcattacgg cagtggcgtc ggtaacggcg ctttatccag
    10141 cgcggcggca cactctgccg tcgggcgatc cacacgctcc atcgtcatac catcgtactc
    10201 aatggtgtta ccgtcacgct caacctcgta cagctcacgt tttacggtaa cgttagtgag
    10261 atcatcagac atcaatgtca atttacccgg cacggcaatc acccgctgcc actgacggca
    10321 atcgagggta tcgccctctt tagtgacaat caggctgcct atcgcttccg gactgaccag
    10381 cgcacgttgc ggccctttgg tctgccagta gcccgccagc cagtccggcg cgggggtttt
    10441 cacgacattg ttatagtttt cgacttcggc acagcccgcc agcaacatga gcgcacctgc
    10501 aattgcgagt tttttcatca ttcgtcctgc atgagaagaa aagaggattg tggcattaaa
    10561 gccctgatgt cgccagcctt tccaggttca gagagaaatt tgatctaacc attatttttg
    10621 tgaattatta ccccgaaaat aaatttgcat ggtgtatgat tcgcgactct tgtttcaaac
    10681 cttctgagtt cagaggctac attcatgtca tggcagcaat tcaaacacgc ctggttgatt
    10741 aaattctggg cgcccatccc cgcggtcatc gcggcgggta ttctctccac ttactatttt
    10801 ggcatcactg gcaccttttg ggctgtcaca ggtgaattta cccgttgggg cggtcagctc
    10861 ctgcagttgt tcggcgtcca tgctgaagag tggggttatt ttaaaattat ccatctggaa
    10921 ggatcgccat taacccgcat cgacggaatg atgatcctcg gtatgtttgg cggctgtttt
    10981 gccgcagcgc tgtgggccaa caatgtcaaa ctgcgcatgc cgcgcagccg tatccgcatt
    11041 atgcaggcca tcattggcgg tattatcgcc ggttttggcg cacgtctggc aatgggctgt
    11101 aacctggcgg cgttctttac cggaattcca cagttctcgc tgcacgcctg gttctttgcc
    11161 atcgccactg ccattggttc atggtttggc gcgcgcttta cccttctgcc catcttccgt
    11221 attcccgtga aaatgcagaa agtttctgct gcctcaccgt tgacgcaaaa accggatcag
    11281 gcgcggcgtc gttttcgtct cgggatgctg gtctttttcg gcctgctggg ctgggcgctg
    11341 ctgacggcga tgaaccagcc caaactgggg ctggcaatgc tgtttggcgt cggctttggt
    11401 ttactgattg aacgtgcgca aatctgcttt acttcggcgt tccgcgatat gtggatcacc
    11461 ggacgtaccc atatggcgaa agcaatcatt atcggtatgg cggtaagtgc catcgggatc
    11521 ttcagttacg tacagttagg cgttgaaccc aaaatcatgt gggcgggacc aaacgcggta
    11581 attggtggtt tactgtttgg ttttggcatc gtgctggcag gcggctgcga aaccggctgg
    11641 atgtaccgcg cggtagaagg ccaggtgcac tactggtggg tcggtctggg caacgtgatc
    11701 ggctcaacga ttctggcgta ctactgggat gatttcgctc cggcgctggc caccgactgg
    11761 gacaaaatca acctgctgaa aacctttggc ccgatgggcg gcctgctggt gacatatttg
    11821 ctgttgtttg ctgctctaat gttgattatt ggctgggaaa aacgcttctt ccgccgtgcg
    11881 gcaccgcaga ctgctaagga gatcgcatga aaaatatcgt tcctgattac cgtcttgata
    11941 tggtgggtga accctgccct tatccggcag tcgcaaccct tgaggcgatg ccgcagttaa
    12001 aaaaagggga gatcctggaa gtggtgagcg actgtccgca gtcgatcaat aatattccac
    12061 tggatgcgcg taatcacggc tatacggtgc tggatattca gcaagacggg ccgaccattc
    12121 gttatttaat tcagaagtaa tccctcattc ccgctgtggc ccacagcggg aatatcgttg
    12181 cctctctatc acatagattt aacccgcccc ctctcctatt cttttcagtg tgacctcagg
    12241 aggtttctat gtgtggacgc tttgcccaat cccaaacgcg tgaagattac cttgcgcttc
    12301 tcgcggaaga tattgaacgc gatattccct acgatcccga acctattggc agatacaacg
    12361 tcgcgccggg aaccaaagtc cttctgctca gtgaacgtga tgaacacctt catctggatc
    12421 cggttttctg gggatatgct cccggatggt gggataaacc gccgctgatt aacgcccgcg
    12481 tagaaactgc cgcaaccagt cacatgttta aaccgctctg gcaacatggt cgggcaatct
    12541 gttttgccga tggctggttt gagtggaaaa aagaaggcga caaaaagcag ccttatttta
    12601 tctatcgtgc tgatggacaa gctgttttta tagccgcgat aggtagcaca ccgtttgagc
    12661 gtggtgacga agccggaaga agtttttgta agcccgctca gggtctttca gtgactgttg
    12721 caatggctgt gagggcgaat ctttaagcca ttgcgtttca gtggcatttt tccactcaac
    12781 caaccaggaa gccattttcc cgtaagggat gtatttatta ccggcctcat gattctcatt
    12841 ctgaagcgcc agagcacgat tgaaaacgaa acgacatgcg cctgcgaagc gcctcatttc
    12901 acgctcctgt tgaccacctg gtcttaaccg gaatttgaat gcctgtagtc gtttcatggg
    12961 ccaaatcata ctttggccta tgaaaaaaga aaccgatatc cgacgcggaa ggcattgtgt
    13021 tttcctgaag catgttcacc tagtcttcgt tactaagaac ctatcccaaa aggaattgtc
    13081 agataataca atatgataat ttctgagggc gaagtgtggc gggcaacaag tggcagatca
    13141 gtgatggact ctgggagaaa atggctccac tcatcccgga gcataaaact caacacccgc
    13201 tgggtacgca ccgcaagcgg gttgataatc gcgctgcaat gaacgccatt ttcttcgtac
    13261 tcaggacggg ctgccaatgg aatgcgctga atgccaccgg tatatgctcg tcaagctctg
    13321 ctcatcgccg atttcaggag tggcgagatg ccggagtatt tgaacgcttc tggtaaaatg
    13381 ggttgcttgc ttgcgaacag ttggactcta ttgactggtc gtggctgtcg atggatggtt
    13441 gtataaccaa atcaccgctg gcaggctcaa aaaaacaggc aagaacccta cagaccgggg
    13501 gaaacagggc gtaaagcgca gtctgatgac tgacgggaac gggctaccgc ttgcactggt
    13561 tgttgccgga gcaaatacgc acgacataaa gttggttacg gatacgctcg atgccctcca
    13621 gacgggcaga ccgggcaaga ggctccggtt gtgcatggac aaagggtatg aagcggaatg
    13681 gctggaatcg tatctgaaaa gtcgtcgcta tgaacctcat atccagtcac gaaaggatga
    13741 gtcagaggcc atcaaataca cggattttaa ggctcaccgc tgggttgtag agagaacaca
    13801 cagttggatg aatcgctacc gccgtgtcct gactcgttgg gagaagaagg tcgagaatta
    13861 tgaggcgatg ctgcattttg cctgtggtgt cattgtctgg aacaaaaccc tattgggata
    13921 ggctctaaat accgacgtca gatttttgac catgacgcga cagaaaaact acgcacttac
    13981 ttttcaaatg tatgtgctga ttttgaagct gaacgggttg aaatggatgg cggaccagat
    14041 cacgtccatt tgttaattaa ctatcctccc aaactggcga tatccagtct ggtaaacagc
    14101 ctcaaaggcg tatcgagtag gttactgcga cgagatcgac cagatattgc agtcaggtat
    14161 tacgacaaag gcgttttgtg gagtcctggc tattttgtca gtagctgcat cttaacgtat
    14221 caactagatt taataacaac gatatccgca cctaaccgga tatctaatgt cataagcatc
    14281 cctttaatta tcccctcaac ctcttgtaac tttcccactc ttgagctcca catagtccaa
    14341 acgccggatg caaaaaaatc acacgtgcat ttctgaaatt gtgatggagg aagatagtat
    14401 caattatatg tgcttagaaa cgtccccgat tagcgcagaa gtcaccggat tgttcagact
    14461 ccaataggtt gagtatgccg tatttatttt ccaaaataaa aatcgatgct gttgctcctt
    14521 cataaaatac ttttactctc tggacgcttt tcttagctct cttggtgtta tcttaaaacg
    14581 attatggaac ctttcagtaa aacgagaagg acacttgtaa ccattttccc tggcaatctc
    14641 gctgatgggt tttactgtcg tttgtatagc agacaacgca ttatttaacc tcacgtcgtc
    14701 caatatactt tgaaaactta ctccctcgct tgccagacgg cgatgcaatg tggaaacaga
    14761 aatgtagaga tatctcgcta ccttgtttgc tgtccatttt gttccaggtt cggatagcat
    14821 taggttataa caacgactta ttaatgattg tttactatct gataaaagta aacaattaac
    14881 atgatgtgct cctaatgaaa gtagaacgcc cattgctaag tgttcctgaa tttgcgttga
    14941 gaagcctcgg gaaatagatg tttttagttg ctcccaacaa tatattaact cggggttctg
    15001 aggtaagcag aaacctgtcg tgttacgaat ttgatcagtt atcgcataaa atttttgaaa
    15061 cttttcaatt agatcaatgg gataataaag catttctgca agataaagac ctgcttctgg
    15121 ataattcgca atataaaatt catatccaca gggaaataat attatctgat tattatcaac
    15181 agttagacta tgcgtctccc aattgataac tttttttccc tgacggatac gacataaagc
    15241 tggcataaga ggcttaaccc tgtgaatctc atgatgttta tgcatccgta tttcttcaat
    15301 ctttaggtta gtcttatctt ttgccagcat actcttaccc tactttatct cataaactgg
    15361 tgttatctca gcggttgcaa ttttattggc attaagcata taaccaacta acgctccgct
    15421 ggaattagaa tccacaggaa ttttatcagt ttttagagcc catactttaa actggtaatg
    15481 atggggttta tctcctttgg gaggacatgc tccaccaaat cctgcataac caaaatcatt
    15541 tcggccttga acagcaccag tcggaagttt tgtaccatca cgtcttcctg catcagcggg
    15601 caaatatgtt acagttgctg gaatattagc aacagtccag tgccaccatc cactgcctgt
    15661 aggagcatct ggatcataca cagttacggc aaagcttttg gtgccttcag gagcaccaga
    15721 ccaggttaat gagggcgatg tattaccacc ttcacaccca aatccagaaa atacatgaga
    15781 cgttgtaagt tgctctcctg tttttatttc attactagtg acctgaaatg ctgcggcctg
    15841 cgcagaaaat gttatgaatg ccaatacact tgaaacgata agttttttca taaaaacctc
    15901 attgttgtga cctatcgtta ttttatttga tgtgccttga tctcattatg cataaaggcg
    15961 caatgatcgc gcaaatgcaa tcataattat aaaggtgccc cgaacattag ccataatatg
    16021 cacaaatcag attcctgtta tctcaaggat aattattttt taatgcggtg cacttcgtga
    16081 caacaagtta atgactaaca caccggcaca aatcaacatc atgcctatga tagctggcag
    16141 gtccagtcgt tgaccaaaaa atccccagga aagcaaacta atcagaacga taccgacgcc
    16201 tgaccagata gcataagcaa tccctgtagg tatataagcc agcgtctgag ctaataacca
    16261 gaatgatgca caatagcaaa taattgtgcc aacagagggc cataaccgtg taaacccctc
    16321 tgaaaacttc attaaggttg taccaatgac ctctgcaagt attgcgccac caagataaat
    16381 ataagggttc atagcatatt ctttcctgtt catactggag agaattgtac tacagtttga
    16441 acacaactca ccgttttcat cctggtgtat gtgtatccag ggggctctgg cgtggcaaga
    16501 gataaaggca ggaacaataa tgatcagaag gaaccccagg ctaacgaatg agttcactct
    16561 tcctgggagc aaagcgaaga caaaacatgt tatcgcgttt acggatcgtg gatggcagaa
    16621 aactttcagg atcaggtatc cattttaaac cagaaattga gtgagtttgc cccatccatg
    16681 ccccacgctg tgggatcgga tataataaac aagcatagtc aacaggttac aaaacctaca
    16741 cctgcatgga catcacttcc tgatacgccg ccaccagctt attacgcacc tgaatcccca
    16801 tttgcataga aactgaggct ttttgcatat cggtcatcac atcgtttagc gccacgccgg
    16861 gctcaccgag ggtgaatttt tctgcctgcg tgcgggcggc tgtttgtgta tcgcttatgc
    16921 gatcgagcgc ggcgtgcagc tgcccggcaa aactaattgt cggttgcggc agtgattcct
    16981 gcgcacgcgc actcatcgcc gtggtctgta actggctgat aaccccttca atcccctgta
    17041 tcgctgacat tttcatctcc cggataattt ctggtagcaa agcctaccag taagtcaata
    17101 agacaaaggc gctaaatagc aacaaaaaaa cgggtttatt ggcggataga aaaaaacgaa
    17161 agcacaaata atgggagcgt caatttttcg agtttgctga cccgggagtg agtcttgttc
    17221 cactttgcca ataacgccgt ccataatcag ccacgaggtg cgcgatgaat gcgactgcag
    17281 cccagacaaa atctcttgag tggcttaatc gcctgcgtgc gaatccgaaa attccattga
    17341 ttgttaccgg ttccgcggca gtggcggtca tggtcgcact gatcctgtgg gcgaaaaccc
    17401 ccgactaccg tacattattc agcaatcttt ccgatcagga tggtggcgca attgtcagcc
    17461 aactgacgca aatgaatatt ccttaccgct tcagcgaagc cagcggcgct attgaggttc
    17521 cggcagataa agttcacgaa ctgcgcctgc gcctggcgca acaaggtttg cccaaaggcg
    17581 gcgcggtcgg tttcgaactg ctcgatcagg aaaagtttgg tatcagccag ttcagcgaac
    17641 aggtgaatta tcagcgggcg ctggaaggcg agctttctcg taccatcgaa actataggcc
    17701 cggtaaaagg ggcgcgcgta catctggcaa tgccgaaacc gtctttattc gtccgtgaac
    17761 aaaaatcccc ttctgcatcg gtgacggtaa atctgttacc cggccgcgca ctcgataaag
    17821 ggcaaattag cgctattgta catctggttt ccagcgcggt tgctggtctg ccgccaggaa
    17881 acgtcacgct ggtggatcag ggcgggcatc tgttaaccca gtccaatacc agcgggcgcg
    17941 atcttaatga cgctcagttg aaatatgcca gcgatgtcga aggccgtatt cagcggcgta
    18001 ttgaagcgat cctgtcgcct attgttggta acggtaatat tcacgcccag gtcacggcgc
    18061 aactggactt cgccagtaaa gaacaaacgg aagaacagta tcgccctaac ggtgatgaat
    18121 ctcatgcggc gcttcgttca cgccagctta atgagagcga gcaaagcggt tccggttatc
    18181 cgggcggcgt accgggggcg ttgtcgaatc aaccggcacc tgcgaataac gcaccaatca
    18241 gcacgcctcc ggcaaatcaa aataaccgcc agcagcaggc gagcaccacc agcaatagtg
    18301 ggccgcgtag cacacagcgg aatgaaacca gtaactacga agtcgatcgc accattcgtc
    18361 ataccaaaat gaacgtgggc gatgtgcaac gtctgtcagt cgcggttgtg gtgaattaca
    18421 aaaccttgcc agacggtaaa ccattgcctc tcagcaacga acagatgaag caaattgaag
    18481 ctctgacccg cgaggcgatg ggcttttctg aaaaacgcgg tgattcgctc aatgttgtta
    18541 actcgccgtt caatagcagt gacgaaagcg gcggagaact gccgttctgg caacagcaag
    18601 cgtttatcga tcagttgctg gctgccggtc gctggttgct ggtactgctg gtggcgtggc
    18661 tgctgtggcg gaaagcggta cgcccgcagt taacacgtcg cgccgaggcg atgaaagctg
    18721 tacagcaaca ggcgcaggcc cgtgaggaag tggaagatgc ggtggaagtc cgcctgagca
    18781 aagacgaaca actccaacaa cgacgcgcta accaacgtct gggggcagaa gtcatgagcc
    18841 agcgtatccg tgaaatatct gataacgatc cgcgcgtggt ggcgctggtc attcgccagt
    18901 ggataaataa cgatcatgag taacctgaca ggcaccgata aaagcgtcat cctgctgatg
    18961 accattggcg aagaccgggc ggcagaggtg ttcaagcacc tctcccagcg cgaagtgcaa
    19021 accctgagcg ctgcaatggc gaacgtcacg cagatctcca acaagcagct aaccgatgtg
    19081 ctggcggagt ttgagcaaga agctgaacag tttgccgcac tgaatatcaa cgccaacgat
    19141 tatctgcgtt cggtattggt caaagctctg ggtgaggaac gtgccgccag cctgctggaa
    19201 gatattctcg aaactcgcga taccgccagc ggtattgaaa cgctcaactt tatggagccg
    19261 cagagtgccg ccgatctgat tcgcgatgag catccgcaaa ttatcgccac cattctggtg
    19321 catctgaagc gcgcccaggc cgccgatatt ctggcgttgt tcgatgaacg tctgcgccac
    19381 gacgtgatgt tgcgtatcgc cacctttggc ggcgtgcagc cagccgcgct ggcggaactg
    19441 accgaagtac tgaatggctt gctcgacggt cagaatctca agcgcagcaa aatgggcggc
    19501 gtgagaacgg cagccgaaat tatcaacctg atgaaaactc agcaggaaga agccgttatt
    19561 accgccgtgc gcgaattcga cggcgagctg gcgcaaaaaa ttatcgacga gatgttcctg
    19621 ttcgaaaatc tggtggatgt cgacgatcgc agcattcagc gtctgttgca ggaagtggat
    19681 tccgaatcgc tgttgatcgc gctgaaaggg gccgagcagc cactgcgcga gaaattcctg
    19741 cgcaatatgt cgcagcgtgc cgccgatatt ctgcgcgacg atctcgccaa ccgtggtccg
    19801 gtgcgtctgt cgcaggtgga aaacgaacag aaagcgattc tgctgattgt gcgccgcctt
    19861 gccgaaactg gcgagatggt aattggcagc ggcgaggata cctatgtctg ataatctgcc
    19921 gtggaaaacc tggacgccgg acgatctcgc gccaccacag gcagagtttg tgcccatggt
    19981 cgagccggaa gaaaccatca ttgaagaggc cgaacccagc cttgagcagc aactggcgca
    20041 actgcaaatg caggcccatg agcaaggtta tcaggcgggg attgccgaag gtcgccagca
    20101 aggtcatgag cagggctatc aggaaggact ggcccggggg ctggagcaag gtctggcaga
    20161 ggcgaagtct caacaagcgc caattcatgc ccggatgcag caactggtca gcgaatttca
    20221 aactaccctt gatgcgcttg atagtgtgat tgcttcgcgc ctgatgcaga tggcgctgga
    20281 ggcggcacgt caggtgatcg gtcagacgcc aacagtggat aactcggcac tgatcaaaca
    20341 gatccaacag ttgttgcagc aagaaccgtt attcagcggt aaaccacagc tgcgcgtgca
    20401 cccggatgat ctgcaacgtg tggatgatat gctcggcgca accttaagtt tgcatggctg
    20461 gcgcttgcgg ggcgatccca cactccatcc tggcggctgt aaagtctccg ccgatgaagg
    20521 cgatctcgac gccagtgtcg ccactcgctg gcaagaactc tgccgtctgg cagcaccagg
    20581 agtggtgtaa tgaccacgcg cctgactcgc tggctaacca cgctggataa ctttgaagcc
    20641 aaaatggcgc agttgcctgc ggtacgtcgc tatgggcgat taacccgcgc taccgggctg
    20701 gtgctggaag ccaccggatt acaattgccg ctcggcgcaa cctgtgtcat tgaacgccag
    20761 aacggcagcg aaacgcacga agtagaaagc gaagtcgttg gctttaacgg tcaacggctg
    20821 tttttaatgc cgctggagga agtggaaggt gtcctgcccg gcgcgcgggt ttatgccaaa
    20881 aacatttcgg ctgaaggact gcaaagcggc aagcagttgc cgctcggtcc ggcgttatta
    20941 ggtcgcgttc tggacggcag cggtaagccg ctcgatggcc tgccctcccc cgatacgacg
    21001 gaaaccggtg cgctgattac cccgccattt aacccgttgc aacgtacacc gattgaacat
    21061 gtgctggaca ccggcgtgcg cccaatcaat gccctgctta ccgttgggcg cgggcagcgt
    21121 atggggctgt ttgccgggtc cggcgttggt aaaagtgtgc tgctggggat gatggcacgt
    21181 tacacccgcg ccgatgtcat tgtcgtgggt ttgattggtg aacgtgggcg cgaagtaaaa
    21241 gattttattg agaatatcct cggtgccgaa gggcgtgcac gctccgtggt gattgccgct
    21301 ccggcggatg tctctccgct tctgcgaatg caaggtgccg cctatgccac gcgcattgcc
    21361 gaagattttc gcgatcgtgg tcagcatgtg ttgctgatta tggactccct cacccgctac
    21421 gcgatggccc agcgtgaaat tgcgctggcg attggcgaac cacccgccac caaaggttat
    21481 cccccgtcgg tgtttgccaa attacctgca ctggtcgagc gtgccggaaa tggcattagc
    21541 ggcggcggat cgattaccgc gttttatacc gtgctcaccg agggcgatga ccagcaggac
    21601 cccattgccg actccgcgcg ggcaatcctc gacggtcaca ttgtgctgtc tcgccgactg
    21661 gcggaagccg ggcactatcc ggctatcgat attgaagcgt cgatcagccg cgcaatgacg
    21721 gcgttgatca gcgagcaaca ttacgcgcga gtgcgcacct tcaaacagct gttgtcgagt
    21781 tttcagcgta accgagatct ggttagcgtc ggcgcgtatg ccaaaggcag cgatccgatg
    21841 ctcgataaag ccatcgccct gtggccgcag ctggagggct atttgcaaca aggcattttt
    21901 gaacgcgcgg actgggaagc gtctctccag gggctggagc gtattttccc gacagtgtca
    21961 taacccagga gataacggca gatggcagaa catggtgcgc tggcgaccct gaaagatctg
    22021 gcagaaaaag aggtagagga tgccgcgcgc ctgctgggtg aaatgcgtcg cggatgtcag
    22081 caggcggaag aacagctcaa aatgctgatc gattatcaga atgaatatcg caataacctc
    22141 aatagcgata tgagtgccgg gatgaccagc aaccgctgga tcaactatca gcagtttatc
    22201 cagacgttgg aaaaagccat tactcagcat cgccagcaac ttaatcagtg gacgcagaaa
    22261 gttgacattg ccctgaacag ctggcgagaa aagaaacaac gtttgcaggc ctggcagaca
    22321 ctgcaggaac ggcaatccac ggcggcactg cttgcagaaa accgcctcga tcagaaaaag
    22381 atggatgagt tcgcccagcg cgccgccatg aggaaacctg aatgattcgc ttagcgccct
    22441 tgattaccgc cgacgttgac accaccacat tgcctggcgg caaagccagc gatgctgcac
    22501 aagattttct cgcgttgttg agcgaagcat tagcaggcga gacaactacc gacaaagcgg
    22561 ccccccagtt gctggtggca acagataagc ccacgacaaa aggcgagccg ctggtcagcg
    22621 agattcttgc cgatgcgcaa caagcggatt tactgatccc tgtggatgaa acaccgcctg
    22681 tcatcaacga cgaacaatcc acatcaacac cattaaccac cgctcaaacg atgacgttgg
    22741 ctgcggtggc tggcaacaat acggcaaaag acgaaaaagc ggatgatctg aatgaagacg
    22801 tcaccgcaag cctgagcgcc ctttttgcga tgttgccggg ttttgacaat acgcccaaag
    22861 tgactgatgt gccgtcaacc gtgttaccgg cagagaaacc aacgctcttc acaaaactga
    22921 cttctgcgca actcacaaca gcacagcctg atgatgcccc cggcacgcca gctcagccat
    22981 taacaccgct ggtagcagaa gcccagagta aagcggaagt catcagcaca ccttcaccgg
    23041 tgaccgctgc cgccagcccg ctaatcactc cacaccagac acagccactg cccaccgtcg
    23101 ccgcgcctgt gttgagtgca ccgctgggtt ctcacgaatg gcaacaatca ttaagccagc
    23161 atatttcgct gttcacccgc caggggcaac aaagtgcaga gttgcgtctg cacccgcagg
    23221 atttaggtga agtgcaaatc tccctcaaag tggatgataa ccaggcgcaa atccagatgg
    23281 tttcaccgca tcaacacgta cgcgccgccc tggaagcagc gcttccggta ctgcgaacgc
    23341 agctggccga aagtggcatt cagttagggc aaagcaacat cagtggcgaa agctttagtg
    23401 gtcagcagca ggccgcttcc cagcaacagc aaagccaacg cacagcaaac catgaacctc
    23461 tggcggggga agacgacgat acgcttccgg ttcccgtctc tttacaagta cgtgtaacag
    23521 gcaacagcgg cgttgatatt ttcgcctaac gtcagaggta gcaccgtaat ccgcgtcttt
    23581 tccccgcttt gttgcgctca agacgcagga taattagccg ataagcagta gcgacacagg
    23641 aagaccgcaa cacatgactg attacgcgat aagcaagaaa agcaagcgat cgctttggat
    23701 cccgattctg gtattcatta ccctcgcggc ctgtgccagc gcaggttaca gctactggca
    23761 ttcgcatcag gttgccgctg acgacaaagc gcagcaacgc gtcgtgccct ccccggtctt
    23821 ctatgcgctg gataccttta cggtcaatct gggcgatgca gatcgcgtac tttatatcgg
    23881 cataaccctg cgcctgaaag atgaagctac ccgctcgcgg ctgagtgagt atttgccgga
    23941 agtccgtagc cgtttgctgt tactgttttc gcgtcaggat gctgctgtac tggcgacaga
    24001 agaaggcaag aaaaacctga ttgccgagat taaaaccacg ctttccaccc cgcttgttgc
    24061 cgggcaaccg aaacaggatg tcaccgacgt gctgtatacc gcttttattc tgcgataacg
    24121 acatgggcga tagtattctt tctcaagctg aaattgatgc gctgttgaat ggtgacagcg
    24181 aagtcaaaga cgaaccgaca gccagtgtta gcggcgaaag tgacattcgt ccgtacgatc
    24241 cgaataccca acgacgggtt gtgcgcgaac gtttgcaggc gctggaaatc attaatgagc
    24301 gctttgcccg ccattttcgt atggggctgt tcaacctgct gcgtcgtagc ccggatataa
    24361 ccgtcggggc catccgcatt cagccgtacc atgaatttgc ccgcaacctg ccggtgccga
    24421 ccaacctgaa ccttatccat ctgaaaccgc tgcgcggcac agggctggtg gtgttttcac
    24481 cgagtctggt gtttatcgcc gtggataacc tgtttggcgg cgatggacgc ttcccgacca
    24541 aagtggaagg tcgcgagttt acccataccg aacagcgcgt catcaaccgc atgttaaagc
    24601 tggcgcttga aggctatagc gacgcctgga aggcgattaa tccgctggaa gttgagtacg
    24661 tgcgttcgga aatgcaggtg aaatttacca atatcaccac ctcgccgaac gacattgtgg
    24721 ttaacacgcc gttccatgtg gagattggca acctgaccgg cgaatttaat atctgcctgc
    24781 cattcagcat gatcgagccg ctacgggaat tgttggttaa cccgccgctg gaaaactcgc
    24841 gtaatgaaga tcagaactgg cgcgataacc tggtgcgcca ggtgcagcat tcacagctgg
    24901 agttggtcgc caactttgcc gatatctcgc tacgcctgtc gcagatttta aaactgaaac
    24961 ccggcgacgt cctgccgata gaaaaacccg accgcatcat cgcccatgtt gacggcgtcc
    25021 cggtgctgac cagtcagtac ggcactctca acggtcagta tgcgttacgg atagaacatt
    25081 tgattaaccc gattttaaat tctctgaacg aggaacagcc caaatgagtg acatgaataa
    25141 tccggccgat gacaacaacg gcgcaatgga cgatctgtgg gctgaagcgt tgagcgaaca
    25201 aaaatcaacc agcagcaaaa gcgctgccga cgcggtgttc cagcaatttg gcggtggtga
    25261 tgtcagcgga acgttgcagg atatcgacct gattatggat attccggtca agctgaccgt
    25321 cgaactgggc cgtacgcgga tgaccatcaa agagctgttg cgtctgacgc aagggtccgt
    25381 cgtggcgctg gacggtctgg cgggcgaacc actggatatt ctgatcaacg gttatttaat
    25441 cgcccagggc gaagtggtgg tcgttgccga taaatatggc gtgcggatca ccgatatcat
    25501 tactccgtct gagcgaatgc gtcgcctgag ccgttagtga tgaataacca cgctactgtg
    25561 caatcttccg cgccggtttc tgctgcgcca ctgctgcagg tgagcggcgc actcatcacc
    25621 attattgccc tgatcctcgc tgctgcctgg ctgataaaac ggctgggatt tgcccctaaa
    25681 cgcactggcg ttaacggtct gaaaattagc gccagtgctt cgctgggcgc gcgtgaacgg
    25741 gttgtggtgg tcgatgtgga agatgcacgg ctggtgctcg gcgttatcgc agggcaaatc
    25801 aatctgctgc ataaacttcc cccttcagca ccaacagaag agataccgca gaccgatttt
    25861 cagtcggtca tgaaaaattt gcttaagcgt agcgggagat cctgatgcgt cgtttattgt
    25921 ctgtcgcacc tgtccttctc tggctgatta cgcccctcgc cttcgcgcaa ctgccgggta
    25981 tcaccagcca gccgctgcct ggcggtggac aaagctggtc gctcccggtg cagacgctgg
    26041 tgttcatcac ctcgttgacg tttattccgg caattttact gatgatgacc agtttcaccc
    26101 gcatcatcat tgtttttggt ttattgcgta acgcgctggg aacaccctcc gcgccaccta
    26161 accaggtatt gctggggctg gcactgtttt tgaccttttt tattatgtca ccggtgatcg
    26221 acaaaattta tgtcgatgcg taccagccat ttagcgaaga gaaaatctca atgcaggagg
    26281 cgctggaaaa aggggcgcag ccgctgcgtg agtttatgct gcgtcagacc cgcgaggcgg
    26341 atttagggtt gtttgccaga ctggcgaata ccggcccgtt gcagggacct gaagccgtgc
    26401 cgatgcgcat tttgctcccg gcctacgtga ccagcgagtt gaaaaccgca tttcagatag
    26461 gcttcacgat tttcattcct tttttgatta tcgacctggt gattgccagc gtgttgatgg
    26521 cattggggat gatgatggtt cccccagcca ccattgctct gccctttaaa ctgatgctgt
    26581 ttgtactggt ggatggttgg caattgctgg tcggttcgct ggcgcagagc ttttacagct
    26641 agagaggcaa aatgacacct gaatcggtca tgatgatggg gactgaagcg atgaaagtcg
    26701 cgctggcact ggctgccccg ctgttgctgg tggcgctggt cacgggtctt atcatcagta
    26761 ttttgcaggc cgccacccag attaacgaaa tgacgctgtc gtttattccg aaaatcatcg
    26821 ccgtatttat cgccattatt attgccggac cgtggatgct caatctgttg ctggattacg
    26881 tccgcacctt gttcactaac ctgccgtata tcatcgggta ggccgtacaa tgatgcaggt
    26941 gacaagcgat caatggcttt cctggttaag cctgtacttc tggccgttac tgcgcgtgct
    27001 ggcgctcatt tctaccgcac caattctgag cgaacgcagc gtgccgaaac gggtaaaact
    27061 ggggctggca atgatgatca cgttcgccat tgccccatca ttacctgcca acgatgttcc
    27121 tgttttttcg ttctttgctc tgtggctggc cgtgcagcag atcctgatcg gcattgcgct
    27181 tggttttacc atgcaatttg cctttgccgc tgtgcgaacc gctggcgaaa ttatcggtct
    27241 gcaaatgggg ctgtcatttg cgacgtttgt cgatccggcc agccatctca atatgcccgt
    27301 tttagcgcgt atcatggata tgctggcgtt actgctgttc ctgacattta acggtcattt
    27361 atggttgatt tcactgctgg tcgatacctt tcacaccctg cctattggtg gcgaaccgtt
    27421 gaacagcaat gcgtttctgg cactcaccaa agcagggagt ttgattttcc ttaacgggct
    27481 gatgctggcg ttaccgctca ttactctgct gctgaccctg aatctggcat taggtttact
    27541 gaatcgtatg gccccgcaat tatccatttt tgttattgga tttccattaa ctctgactgt
    27601 cggtatctct ttaatggcgg cattaatgcc gttaattgcg cctttttgcg aacatttatt
    27661 cagtgaaatt tttaatttgc tggctgatat tattagtgaa ttgccattaa tataattccg
    27721 taacgtttat catgttatcc taaggattat ccgaaaaata atacctacga acatcttcca
    27781 agatactcct gcagcgaaat atttgtttta agctcactca catatcgcaa catttacttt
    27841 actttaagac aattccaggc aaattataca acactttacg ggatagtaag tccgcctgaa
    27901 aaatcgcgag agtggcgcat taggtgaccc atgttgttcc gtttagtcat gatgaaatat
    27961 tcaggtaagg ggaattatcg ttacgcattg agtgagggta tgccatgtca acgattatta
    28021 tggatttatg tagttacacc cgactaggtt taaccgggta tctgttgagt agaggggtta
    28081 aaaaaagaga aatcaacgac attgaaaccg ttgatgacct tgccatagct tgtgattcac
    28141 agcgcccttc agtggtgttt attaatgagg actgtttcat ccatgatgct tctaacagtc
    28201 agcacatcaa gcacatcatt aatcaacatc ccaatacgtt atttatcgtt tttatggcaa
    28261 ttgccaatgt tcattttgat gaatatctat tggtcagaaa aaatttattg ataagttcta
    28321 aatcgattaa accggaatct ctcgacgata tccttggcga tattctgaaa aaagagacaa
    28381 cgataacctc gtttttaaat atgccgacgt tatcattgag ccgaaccgaa tcgagtatgt
    28441 tgcgaatgtg gatggcaggt cagggaacca ttcaaatctc tgaccaaatg aatatcaaag
    28501 ccaagaccgt ttcatcgcat aaaggtaata ttaaacgtaa gatcaaaacg cataataaac
    28561 aggttatcta ccatgtcgtc cgactgacgg ataatgtgac taatggtatt tttgtcaaca
    28621 tgcgctaaca cattctgact ggtggtttcc caccagtcag gctgaataag attactctgc
    28681 tttctccaca aagataccgt cctgatgccc tgcttcatta aaaaaccaga tgccgagcgg
    28741 gtagtcttcc agcgaaacca ggtacattgt gccttcacta aactcctcaa ctgccagtac
    28801 cacgccagga cgacgcggac cgccatccgt tttgactgtt acccgatcat tcaccttcat
    28861 cgttttcctc ctgtgccttt gtgccagtgt agaacaattt cgtttttctg gcagcgccag
    28921 gcgcgcgcga gtgctgattt tctcgacggt ctatacttaa gtgatgccag gcggacttaa
    28981 cgactggcgg caacaacaga gtaacggttg cgaggaaaga tgatgaaaac cgcaaaagag
    29041 tacagcgata ccgcaaaacg tgaggtcagc gtcgatgtcg atgccctgct ggcggcgatc
    29101 aatgaaatta gcgaaagcga agttcatcgc agccagaacg attctgaaca cgttagcgtc
    29161 gatggacgtg aatatcatac atggcgtgaa ttggcggatg ccttcgaact ggatattcat
    29221 gacttcagcg tctctgaggt gaatcgttga atgcacaata aaaaaatccc gaccctgagg
    29281 gggtcgggat gaaacttgct taagcaagaa gcacttaaaa aattcgttac accaggaaat
    29341 ctgatgtgtt catcacctta tccgcaattt ttttcgctga caagaaaata ttcgctatat
    29401 gaatgattaa cgctacttat gaatagaaat gtgacgcacg tcaccccttg gtggctctgg
    29461 cgtcagcggg atcgctcccg aacaaaatca tctttgataa tttgtgctgt tttgagaatc
    29521 atcctggtaa ggggagtact gcgcgactat gttttcaatt caacaaccac tactggtttt
    29581 tagcgatctt gatggcaccc tgctggacag tcatagttat gactggcaac cggcagcccc
    29641 ctggctcacc cgtttacgcg aagcaaatgt tcccgtcatt ctctgtagca gtaaaacatc
    29701 agcggaaatg ctgtacttgc aaaaaacgtt ggggctacaa ggtttaccgc tgattgccga
    29761 aaatggcgca gtgatccagc ttgctgagca atggcaggag atagacggtt ttccacgcat
    29821 catctcaggt attagccatg gcgaaatcag ccaggtttta aatacgctac gcgagaaaga
    29881 acattttaaa ttcacgactt ttgatgatgt cgacgatgca actatcgccg aatggacggg
    29941 attaagccgt agccaggcgg cgctgacgca gcttcatgag gcgtcggtaa cgctaatctg
    30001 gcgcgacagt gacgagcgta tggcacaatt taccgctcgt ctgaacgaac tgggcttaca
    30061 gtttatgcaa ggtgcgcgct tctggcacgt cctggatgcc tctgccggaa aagatcaggc
    30121 tgccaactgg attatcgcga cctatcaaca attgtcaggc aaacgcccaa ccacacttgg
    30181 cctgggcgat gggccaaacg atgcgccctt actggaggta atggattacg cggtgattgt
    30241 gaaagggcta aaccgtgaag gggtgcatct gcatgatgag gatccggccc gcgtctggcg
    30301 aacgcagcgt gaaggaccgg aaggatggcg tgaagggctg gaccattttt tctccgcccg
    30361 ttaagcgtta tcgctcgcga atacccgatt acgcccagcc tgtttagcga gataaagccg
    30421 acggtcggcc agtgactgga attgttcaaa atcataatca ccggtttcct cgctgctact
    30481 tacccccaac gaggcactga tgcgtatcgt cgtactctta gcaatcaaca tctctttttc
    30541 atttaacttc aggcgaatac gttctgcgac ttccgcagcc tccgtcagac tcgcgcctgg
    30601 cagaatcaca caaaactcct caccaccgac ccgcccggca acgtcctgcg cacgcaagga
    30661 actgctaatt aatccggcag catgagaaag aacacggtcg cccgcctgat gaccaaagcg
    30721 gtcattaatc gctttaaaat ggtcaagatc gacctgaatg acagaaaaag gatgttggtg
    30781 cgtctgacac aatttagcga gcggacgggc tttttcgaac agtgcgccac gattatataa
    30841 acgggttaag gtgtcgtgcc acgcctgcca ctgcaacgaa ctttgcagaa catacatgtt
    30901 gctgaccatc cggcgaatca cataccagga gatgagtaac atggtggtaa agagcgccca
    30961 cagcagggtt aatgcaatgc tgatactgcc gaaatcgccg cgcacgcctt cgcttaacgt
    31021 atggacgcgc accagcacac cgtcgaaatg gtccagacgt tcccagctaa catagcgact
    31081 gtccatacga atgccgcccc gcgtgtcatg ttccattgcc tacgccagca aggccagttc
    31141 acgaggatca aaaatattcc ctgttggatg gtcaggattg gaagaggtca aaaatctcag
    31201 cttactgtca tagagctgat actcaccatc gaggttttta tcgatggcgt ttcttaaaaa
    31261 ttgctgcata gtacgcacgg gaatactcat ccccagcacg ccataccagt agttattact
    31321 gtctaccgga acactgacgg taacctgcgg ttcagtattg ctggcgtgtt ccggttgcga
    31381 agtgaaccag cgtaccgcgc ggtgacgatt ttctcgttgt gaatggccaa taaaccaggg
    31441 ttgggtgaca tagccgtaat aacgcgttgg aacattgcgc gtaaacaagg tcggctgcgt
    31501 cgaaacgtaa aatccggcac gcgagacata catcgcctgt tctaccatcg acgaggagtt
    31561 gtgtgccagt cgcagcaagt aaccaacttc cagtgcagcg gtaatttcat tgtcgaggct
    31621 ttcattttcg cgagacagga gattcccctc gctgactaat gcatccgaca cgccattgac
    31681 tggcagggtg cgtcgtcggt tgagttcgat ttgccaggcg tgctcgtcgc gatgctgttc
    31741 gaactcggtt acagcatcac gcagagaggt gaaatccagt ggcgctacga gagcttcgca
    31801 catgccatta cgcaaaaaga tcagtttgtc gacgttatac tgcaaatgct tatcgagcgc
    31861 gttggctacg ttttccagat gattacgctg gctggagata taggcatctt ccagcactac
    31921 cacttcacgc caggtcagca aggtggaaaa aagcaatacc acgataaagc agagattaac
    31981 gatatgacca agccccaggc ggcgtgcgag ttttttcaac cagctctggt tttccatttt
    32041 tgtctcgtgc tgcaccctga cgactcccca cctgcttttt tatgattctg gcataacctg
    32101 gcgatagcgt accgcaaaac agcgataatt ttataatggt ggcatgataa gacgcgacta
    32161 tcattcattg caggcattgg cacaccaggc cggaagtgat ccggcctggt gttaatgata
    32221 ccctttcaaa cttcttccgc agaacgactc ccctcccccg gaacaagctc ggtttcatga
    32281 aaagcttccc gcttgacccc gtaaccgtca taccaacgac actcaaccat accgctggag
    32341 tatccagtga caatcatccg cgggccgccc tctttaaccg taacttcctc actaaccata
    32401 aagctcatac tcgcctcctt ttttcgagtg aaacttgttc accttagttg aagatggcga
    32461 attttgcata atagcctgtg cgagtattaa tgtgcctgac cgcgcatttt cgctacggct
    32521 ttcactccca gcaccacgat gccgccgatg ataaatccaa gaatcagatt taaaacagtc
    32581 ggtaatatca tcgccaccac tgcactttgc tgcccggcga aatgttcaat ggcgtgatgc
    32641 agcggcgcaa taccatgtac cacaataccg ccgccgacga ggaacatcgc cagcgtgccg
    32701 acaatcgata acgccttcat caaccagggc gcaatgatca ataacccttt acctaatgcc
    32761 tgcatcagcg cgctggattt ttccgccagc caatacccca ggtcatcaat cttaacgata
    32821 acccctacca gaccgtagac gcccacggtc actaccagcg cgatgcctga aagcaccagc
    32881 acctgattaa gcaacggcgc ttcggccaca atccccagcg tgatggcgac gatttcggca
    32941 gacaatataa aatcggtacg aatcgcccct tttattttgt ccttttcaaa cttcagcgga
    33001 tcctgcgccg ccagcttctc cagacgctgc tggctctgcg ccggatcttc tttatgttta
    33061 cgcgcctcca gcatatgcag cactttctct actccttcaa agcagagaaa cgcgccacca
    33121 atcatcaaca gaggcgtaat cgcccacggg ataaacgcac tgatgatcag cgccagcggc
    33181 accagaatca ctttattaat cagcgatcct ttcgccacgc cccagaccac gggaagttcc
    33241 cggttggccc gcacgcctga aacttgttgc gcattgagcg ataagtcatc ccctaataca
    33301 ccggcggttt tcttcgccgc cagtttgccc atcacggaga tatcgtccag tagtgtggcg
    33361 atatcatcga gcaacgtcag taaactactc ccggccaaaa gcattccctc tcatttttat
    33421 tgttgaaggg tgtaagtatg aagcaaaaac ggcagctctg atacttgctt cacaggtcaa
    33481 cattttttta acaatcaagt gcaattatat ctctcgccag cagaatgatt ttcacgttta
    33541 ctatgaacca cctttttatt tccaccgtga gggattatgc gtttccgcca gttgttaccg
    33601 ctttttggcg cgctgtttgc gttgtatatc atttggggct caacctattt tgtcattcgg
    33661 attggcgtgg aaagctggcc tccgttaatg atggcgggcg ttcgattcct ggcagccggt
    33721 attttattgc tggcattttt gctactgcgc ggacacaaac tccccccgct acgtccgctg
    33781 ctcaatgccg cggtgattgg cctgttattg ctggctgtcg gtaatggcat ggtgacggtt
    33841 gccgagcatc aaaatgttcc ttccggcatc gccgccgttg tggttgcaac cgtgcccctc
    33901 tttaccctgt gcttcagccg cctgtttggc attaaaacgc gcaaactgga atgggtgggt
    33961 atcgccattg ggcttgccgg aatcatcatg ctcaatagcg gtggaaattt aagcggcaat
    34021 ccgtggggcg cgattctgat tttaatcggc tcgattagct gggcgtttgg ctcggtttat
    34081 ggctcgcgca ttaccttacc tgtagggatg atggcgggtg cgattgagat gctggcggca
    34141 ggcgtggtgt taatgatcgc gtcgatgatt gcaggtgaaa aactgacggc actcccttcc
    34201 ctttcaggct tccttgcggt cggctatctg gcgctgtttg gttcgattat cgccattaac
    34261 gcttatatgt atttaatccg caacgtcagt ccggctctcg ccaccagcta cgcctacgtt
    34321 aacccggtgg tcgcggtctt gctgggtacg ggactgggcg gagaaacgct gtcgaagatt
    34381 gaatggctgg cgctcggcgt aattgtcttc gcggtggtac tggtcacgtt gggaaaatat
    34441 ctctttccgg caaaacccgt agttgcgcca gttattcagg acgcatcaag cgagtaaatg
    34501 aatcccctgc gtgtcgatct gcgcgctggc accttcgccg cagatccact cttccagacg
    34561 ctcgataagc gcttcatccg tcagcttctc gcgcccacgt aacgcacact cccagacaat
    34621 cagtacgcgc cagccgagct cctgcaagcg actgatatcg cggcgatcgc gctcaacatt
    34681 tttacctatc ttctccagcc agaactcagt tcgagtcgca ggcactttaa acagatagca
    34741 gtgatgatgg tgccagaagc agccgtgggt aaatatcacg caacgatatt catcaacgac
    34801 aaaatccgga cgtccgggca gactggcgtc ctgaacgcga aatgccaggc cctgcccggt
    34861 taacagactg gcgaggcgct tctctatcgc cgtatcacgc gtggcaatcg cgcgcatatt
    34921 tttgctgcga gtggccttat cgtgaacgtc ggccatgttg tgcctcttgc tgacgcaacg
    34981 ccaccgcctg tttgattttt ggctcaagca gttttgccac cgcggcaaag accggcacga
    35041 ccaccgagtt accgaactgg cgataggcct gagtgtccga aaccggaatg cggaatttcg
    35101 cttctcccgg cgcttcaaaa cccattaagc gcgcgcattc ccgaggcgtt aaccgacgtg
    35161 gacgatgttg ctgattcagc ggatcgtcaa agtctttttc acccgtggcc atatcccatc
    35221 cgcgatcgat taaaatttcc gcgccatctt tgtaataacg cgcagacagc gtacgggtga
    35281 cgctttgcgg attgttcgga taaaccattc cataaccgaa gccgttaccg cgcgcctgat
    35341 gttttttcgc atatcgatag aggtacttcc acagtaccgg cgtcaggata tatttcgcct
    35401 cgaccatcgg atccagcagc tgcgccagcg tcactcgctg cgcagggaaa catccgctga
    35461 tatcacgcag agtaaaatcg gctttcagat taagatcgcg acgaaaaccc accagcacga
    35521 tgcgttcacg gtgctgcggc agaaaatgtt taccatcgat gattttcgga tcgtccggcc
    35581 cgttatcttc tgcatcagcc acgtcatagc ccagttcgtc cagcgtctgc atgatgatgc
    35641 ggaacgtttt accctggtcg tggcttttca ggtttttgac gttttcgagc acaaacatcg
    35701 ccggacgacg cgcgtcgata atgcgcacca catcaaagaa cagcgtgccc tgagtatcgc
    35761 aggcaaaacc gtgcgcccgc ccgagcgagt tctttttcga tacgccagcc agcgaaaatg
    35821 gctgacaagg gaaaccggcc agtaaaacat cgtgttcagg aatgtgttga cgaatatgtt
    35881 ccgccgccgc ctcatcactc acaccttctt tatggctgag ggttatgtcg cggatatctt
    35941 cattaaaatg atgcgtcgcc gggtcgcaat aatggttggc tttataagtg cgtaccgcat
    36001 gtttgttcca ttcgctggta aacacgcatt gtccgccaat cgattcaaaa ccgcgacgga
    36061 taccgccaat tccggcaaat agatcgataa agcgaaacgc ataatgcgga tgatgtgccg
    36121 gtggtttggg taacaacgtt tgcagatggg cgaactcttt ctcacttaaa cggtgccatg
    36181 ccgagtcatt cgccagcgca cgttttaaaa ttgccgcgct ccagtgattc tcacccacac
    36241 cattaagctg cgccaccagc gttttaacat cataaatttg cagcagtttc tccagcattg
    36301 cctgtgcggc attcccggtg ctgtatgaat cggttactga tatattttcc tgcatagatt
    36361 taaccggcca tctgagatgg ccgacaggtt aacataatta cagccattta gggccacgcg
    36421 ctcagcccgc aggtgaaaac gcatctatca ccttgtgatc gacaccttca ttctcccccg
    36481 ccagttcggc actgagcttc gccataaact cgactaaaaa ttgcgcgttg tgctgcgcca
    36541 gctgcttgcc tcttacggtt tgcatggttt gcggtagctt cagcaactta gtctggaaat
    36601 gatccagcgc gtagcgttta tcatcaagcg ggcgatactg tgcgaacgga tcttcgccat
    36661 caaacagcgc cacgcccaat gcccctgaaa cggcaaacac gcgcgccagg ccaatcgccc
    36721 ccagggcttc cagccgatcc gcatcctgga cgattttagc ttccgttgtt aagggggcga
    36781 tttgcgcgct gaaactgtga gcggcaatgg catgacaaac ggcctcgatt ttctccgccg
    36841 gaaattgctc gaactcttca cggagcaggc gacgcgtctc ttctgccgcc aggattgaag
    36901 aacgctgccg ttgcggatga tttttcgcca ggctcacaat atcgtggaaa taacaggcgg
    36961 ttaaaatcac cagcatatcg acgtcatcat ctgccgccag cttctgtgcc gtagcccaga
    37021 cgcggcgaaa atgatacacg tcatgggccg cgtcctggtg ctgatgatgg tttttcaacc
    37081 agttttcgaa ctgtgtctgc cagtgttgta agtccatacg cgctccgttg ccaaagagtc
    37141 gccaccttag cagtttttat tcatagcgtc tgctacgtgc gggacgaggt cgggtgtacc
    37201 aggcaatgct gcctaacaat gccccgacca gagccataaa taaaaatccg accagtgccc
    37261 ctaaccactt tcccgcggta gagcctaaat cgctttcaaa tcccgacacg ggggcatcgg
    37321 ggatccggct catcacccag atgtaacgcg ccattgccca gacgaaatag ccacaccatg
    37381 tatagaaagc tcacaaagcc agtttgccac cagggctgcg agagagcctc tcttccatct
    37441 ttgtcgaacc gtattccatt ttgagataac ccggaaaatt cagacttagt actattatgt
    37501 gataaaagtc acatttttcc acattgaatg agccagtcaa gactcaatca ttttttgaaa
    37561 aaatagataa attttgcgca acggcagtga atacaggaaa aatcgt
//
LOCUS       DAPHCV010000042        36651 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.042, whole genome shotgun sequence.
ACCESSION   DAPHCV010000042 DAPHCV010000000
VERSION     DAPHCV010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.042"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(48..329)
                     /locus_tag="RH033_002072"
     CDS             complement(48..329)
                     /locus_tag="RH033_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HDV2991254.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(592..1701)
                     /gene="apbC"
                     /locus_tag="RH033_002073"
     CDS             complement(592..1701)
                     /gene="apbC"
                     /locus_tag="RH033_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HDV2991255.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1833..3866
                     /gene="metG"
                     /locus_tag="RH033_002074"
     CDS             1833..3866
                     /gene="metG"
                     /locus_tag="RH033_002074"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDV2991256.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLDHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4007..7801
                     /locus_tag="RH033_002075"
     CDS             4007..7801
                     /locus_tag="RH033_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HDV2991257.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQIDIEWDYANNVIIILPSGIAPSYLEQYSRFELR
                     LRKHLSLTEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEIAQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVAALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGQTKRCHDRMTKAIAAFPHAAMAALTELLGQK
                     EENSWRIMLMTMLISQPALAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAVV
                     VSPPWLSKKKKSPIPVLDLAPLGIEPICYLTEEISNQLLAKYIWYSKHITVSHEESTT
                     NLLARMGFQRRIAGTYIKAPEAVVEAWLNEDYSTLLSEFKVFHSPTGHYWQLGILTTL
                     PLEKAVKAWNALTLSPHTDTEYSMLHFGLKGLPGLVNSLARYPQEALPITNYFAASEL
                     APAVARAFNKLKTLRENARSWLLKYPEHALTGLLPAALGKAGEAQDNARAALRMLTEN
                     GHQPLLQEIARRYNQPEVTDAVNALLALDPLDNHPTKIPTLPAFYQPSLWTRPVLKAN
                     AQSLPDSALLHLGEMLRFPQEEALYPGLLQVKDVCSADSLAGFAWDLFTAWQTAGAPS
                     KESWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNG
                     IAQKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFT
                     VSFDETLKPFVRDVSGSRLKDLPKPNKSDDETRANDAVNRYKLLKKDARTIAAQQVAR
                     LESAMCLRRRWSLENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSSST
                     ADDDLFTLPEGDISIGTPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAE
                     RNASELTRWAGRKCPSGRVMGLANKGWIKGEPQDGGWIGWMIKPLGRWSLIMEIDEGF
                     AVGMSPAELSAEQLLSKLWLWEGKAERYGWGSNSTQEAQFSVIDAITASELINDIEAL
                     FE"
     gene            7811..11443
                     /locus_tag="RH033_002076"
     CDS             7811..11443
                     /locus_tag="RH033_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HDV2991258.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRRWPGEPVSVITGSLIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSIPDRTMAALACIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFIARCYESDPLVVTLQYQDEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALILPEKPEIANELVGL
                     ECPRTHFHSKEWLKVVANDPTAVRKLEHYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAIPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYHKNFP
                     HATLAALAELLALTEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAAE
                     VMPWLSTQPQSVLKSYLSAPPKPVIDGTDNSNLPEILVSPPWRSKKKMTAPRLDLAPL
                     ELTPQVYWQPGEQERLAATEPARYFSTESLAQRMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLFVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHIEALINIWEND
                     NYSHYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVAHVWRRFAAQRDLARQWILQWPEHTASALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWSALEQLLKQGPMDIYPA
                     RIPKAPDFWHPQMWSRPRLITNNQTVTNDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLETALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRCLLKAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLF
                     ADYEIMPPFRQLSRRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYNLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11504..11821
                     /locus_tag="RH033_002077"
     CDS             11504..11821
                     /locus_tag="RH033_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2991259.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKYSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
     gene            12128..13216
                     /locus_tag="RH033_002078"
     CDS             12128..13216
                     /locus_tag="RH033_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDV2991260.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDEAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVECIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            13227..15506
                     /locus_tag="RH033_002079"
     CDS             13227..15506
                     /locus_tag="RH033_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2991261.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQDESQALLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDASGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQKIN
                     KPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQKAGEQLLKT
                     ILTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFTPDGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARNKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCATIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLPGVSLSAAVALLERRSQAIHAPALDRGAALGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     EADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQNWGVFHV"
     gene            15499..16635
                     /locus_tag="RH033_002080"
     CDS             15499..16635
                     /locus_tag="RH033_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDV2991262.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVCQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            16632..18632
                     /locus_tag="RH033_002081"
     CDS             16632..18632
                     /locus_tag="RH033_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HDV2991263.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIDGTLCEHVVLAVQAFVEAKAQQAEFNHLIWQMRSEHVTSSDDPFASEEG
                     NACRQYVQQLSQTLWLGGISQPLIHYEAAFNRALQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVHPMPWRTVVGAGIAGEAKL
                     DHLRLVSLGMRCWQDIEQYGLRIWFTDPDTGSILHLSRSWQRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNVLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVDGQLTLEPQIMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAIGQAELLANDLTAV
                     GFYRLAHVLAQFRNTESDARVEAMNNGVLLCEQLFPMLQQQG"
     gene            18757..19218
                     /locus_tag="RH033_002082"
     CDS             18757..19218
                     /locus_tag="RH033_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HDV2991264.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAARTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(19260..19730)
                     /locus_tag="RH033_002083"
     CDS             complement(19260..19730)
                     /locus_tag="RH033_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HDV2991265.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(19777..20496)
                     /gene="yehT"
                     /locus_tag="RH033_002084"
     CDS             complement(19777..20496)
                     /gene="yehT"
                     /locus_tag="RH033_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HDV2991266.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(20493..22178)
                     /gene="btsS"
                     /locus_tag="RH033_002085"
     CDS             complement(20493..22178)
                     /gene="btsS"
                     /locus_tag="RH033_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HDV2991267.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDCYTRITLRLPWRDEA"
     gene            22400..23131
                     /gene="mlrA"
                     /locus_tag="RH033_002086"
     CDS             22400..23131
                     /gene="mlrA"
                     /locus_tag="RH033_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HDV2991268.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYISICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGD
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            23191..23298
                     /locus_tag="RH033_002087"
     CDS             23191..23298
                     /locus_tag="RH033_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HDV2991269.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(23279..24010)
                     /gene="yehW"
                     /locus_tag="RH033_002088"
     CDS             complement(23279..24010)
                     /gene="yehW"
                     /locus_tag="RH033_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HDV2991270.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGQQPAIIALILYGVLPVLQATLAGLGSIDASVTEVAKGMGMSCSQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGANTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQAFSQHAK"
     gene            complement(24015..24941)
                     /gene="yehX"
                     /locus_tag="RH033_002089"
     CDS             complement(24015..24941)
                     /gene="yehX"
                     /locus_tag="RH033_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HDV2991271.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLEPNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMGHGEVVQQGN
                     PLTMLTRPANDFVRQFFGHSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(24934..26091)
                     /gene="yehY"
                     /locus_tag="RH033_002090"
     CDS             complement(24934..26091)
                     /gene="yehY"
                     /locus_tag="RH033_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HDV2991272.1"
                     /translation="MTYFRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWMTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGALNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVILVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(26098..27015)
                     /gene="osmF"
                     /locus_tag="RH033_002091"
     CDS             complement(26098..27015)
                     /gene="osmF"
                     /locus_tag="RH033_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HDV2991273.1"
                     /translation="MPLSKVWAGSLVLLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDTAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLSQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDEKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(27205..29502)
                     /gene="bglX"
                     /locus_tag="RH033_002092"
     CDS             complement(27205..29502)
                     /gene="bglX"
                     /locus_tag="RH033_002092"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HDV2991274.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            29698..31413
                     /gene="dld"
                     /locus_tag="RH033_002093"
     CDS             29698..31413
                     /gene="dld"
                     /locus_tag="RH033_002093"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDV2991275.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHILGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     VLKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(31452..32384)
                     /gene="pbpG"
                     /locus_tag="RH033_002094"
     CDS             complement(31452..32384)
                     /gene="pbpG"
                     /locus_tag="RH033_002094"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HDV2991276.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(32558..33145)
                     /locus_tag="RH033_002095"
     CDS             complement(32558..33145)
                     /locus_tag="RH033_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HDV2991277.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            33315..33893
                     /locus_tag="RH033_002096"
     CDS             33315..33893
                     /locus_tag="RH033_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV2991278.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(34023..34784)
                     /locus_tag="RH033_002097"
     CDS             complement(34023..34784)
                     /locus_tag="RH033_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV2991279.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGARAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDGDVKPDAEPSIPLRRFGTTHEIA
                     SLVVWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(34837..36273)
                     /gene="mdtQ"
                     /locus_tag="RH033_002098"
     CDS             complement(34837..36273)
                     /gene="mdtQ"
                     /locus_tag="RH033_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HDV2991280.1"
                     /translation="MNRDSFYPAIACFLLLLMLAGCAPMHETRQALSQQTPASQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNHQTKALKLNP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVDAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            36497..36580
                     /gene="yohP"
                     /locus_tag="RH033_002099"
     CDS             36497..36580
                     /gene="yohP"
                     /locus_tag="RH033_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HDV2991281.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         9042 a         9754 c         9241 g         8614 t
ORIGIN      
        1 ataaatgatt tcggctttct aaatgcaatt aaaatcaggt tgccaaatta atcgtagcga
       61 tttaacacga ttcgacgttt gctatcgcta cctgcaacaa ccacttctgt cgttacacct
      121 ttgacgggcg aagaaaacag atgacgggtg tcaacattat acgttaactg accacaatgc
      181 tgttgaggtt gaccattaac catagccata ctgcatgttg ggggactgat cctgccatta
      241 atcgctaatg tggcagtctc tgaagagaat gcgggagaag ccaatccata ggcaagaaaa
      301 ataattcctg acagccaata cttattcatt gaacgttatc cctgtagtaa aggttatgcc
      361 tggatagaat gagtgcataa caaactatag ctgtacatcc actacacagc cacgaaggat
      421 gataatgaag cattgcctgt atgatcaatc gactttgtag agtttcggac gaaggtccgc
      481 agaatattcg cagtattaaa taagtgttca ttaatgcggc aaaaactaat acaccgcatc
      541 aatgtaacat ctctgcggtg catgaagaat aaccagaatg gttaataggc gttagaccgc
      601 gcggaaggaa atctcgcctg gaatgacttc accctgccag tagagctgag ctgcaacgcg
      661 gtcagccagc tggcggtaga tagcggtaaa ttcgctctcc ggacggctga taaccgttgg
      721 cgtgccttta tcgagatcct cacgtaagga gatgtgtaac ggcatctgcc ccagcagctg
      781 tgtgtggtat ttttcagcca gtttctctgc accaccggta ccgaaaatcg gttcatggtg
      841 accacagtta ctgcaaatat gcacactcat gttctcgaca ataccaagca ccggaacctc
      901 gactttctcg aacatcacaa tgcctttttt cgcatcgatc aacgcgatgt cctgcggggt
      961 ggtgaccacg actgcaccag ttactggaat gttttgcgcc agcgtcaatt gaatgtcacc
     1021 ggtgcctggc ggcatatcaa gcaccagata gtcgagatcc ggccacaagg tttcctgcaa
     1081 catctgcatt aacgccttgc tcgccatcgg tccacgccac accatcgcat tgtcgtcagt
     1141 gaccagataa ccaattgaat tggttgccag gccatgagac ataatcggtg ccatatgggt
     1201 accgtccggc gaagttggac gctgattttc tgcgcccagc atggtgggaa ttgatgggcc
     1261 gtagatatcg gcgtccagaa tgccgacttt cgccccttcc gccgccagtg ccagcgccag
     1321 gtttaccgcc gtggaggatt tacccacgcc gcctttaccg gaactgaccg caatgatgtt
     1381 cttcacacca ttaatacccg gctgattctt cacgcgtttt aaggtggcga tattgtgcga
     1441 cagtttccag tcgatagctt tcgccccggt gatacgcagc agttcggcgc tacattgctc
     1501 ttttaactct tcaaacgcgc tatgccagac gaatggcata accagttcca catgcaacgt
     1561 gtcgtccatc caggcgacat ggtgcaacgc tttcagcgtg gtgaggttat gcttcagggt
     1621 tgggtgctga aaattggcca gtgtcccggc gaccattgcg cgcaaggctt ctggtgattt
     1681 ggcctgggat tgttcgttca tcccgactcc ttttttgtat aaataaacca tcagctgata
     1741 gtttacctga agaatataga gaagtactta cttaacattt tcccatttgg tactatctaa
     1801 ccccttttca ctattaagaa gtaatgccta ctatgactca agtcgcgaag aaaattctgg
     1861 tgacgtgcgc actgccgtac gctaacggct caatccacct cggccatatg ctggagcaca
     1921 tccaggctga tgtctgggtc cgttaccagc gaatgcgcgg ccacgaggtc aacttcatct
     1981 gcgccgacga tgcccacggt acaccgatca tgctgaaagc tcagcagctt ggtatcaccc
     2041 cggagcagat gattggcgaa atgagtcagg agcatcagac tgatttcgca ggctttaaca
     2101 tcagctatga caactatcac tcgacgcaca gcgaagagaa ccgccagttg tcagaactta
     2161 tctactctcg cctgaaagaa aacggtttta ttaaaaaccg caccatctct cagctgtacg
     2221 atccggaaaa aggcatgttc ctgccggacc gttttgtgaa aggcacctgc ccgaaatgta
     2281 aatccccgga tcaatacggc gataactgcg aagtctgcgg cgcgacctac agcccgactg
     2341 aactgatcga gccgaaatcg gtggtttctg gcgctacgcc ggtaatgcgt gattctgaac
     2401 acttcttctt tgatctgccc tctttcagcg aaatgttgca ggcatggacc cgcagcggtg
     2461 cgttgcagga gcaggtggca aataaaatgc aggagtggtt tgaatctggc ctgcaacagt
     2521 gggatatctc ccgcgacgcc ccttacttcg gttttgaaat tccgaacgcg ccgggcaaat
     2581 atttctacgt ctggctggac gcaccgattg gctacatggg ttctttcaag aatctgtgcg
     2641 acaagcgcgg cgacagcgta agcttcgatg aatactggaa gaaagactcc accgccgagc
     2701 tgtaccactt catcggtaaa gatattgttt acttccacag cctgttctgg cctgccatgc
     2761 tggaaggcag caacttccgc aagccgacca acctgtttgt tcatggctat gtgacggtga
     2821 acggcgcgaa gatgtccaag tctcgcggca cctttattaa agccagcacc tggctgaatc
     2881 attttgacgc tgacagcctg cgttactact acactgcgaa actctcttcg cgcattgatg
     2941 atatcgatct caacctggag gatttcgttc agcgtgtgaa tgccgatatc gtgaataaag
     3001 tggttaacct ggcctcccgc aacgcaggct ttatcaacaa gcgttttgac ggtgtgctgg
     3061 caagcgagct ggctgaccct gagctgtaca aaacctttac cgatgccgct gaagtgattg
     3121 gtgaagcgtg ggaaagccgt gaatttggta aagcagtgcg cgaaatcatg gcactggctg
     3181 acctggctaa ccgctatgtc gacgagcagg ctccgtgggt agtcgcaaaa caggaaggtc
     3241 gcgatgctga cctgcaggcg atttgctcaa tgggcatcaa cctgttccgc gtgctgatga
     3301 cttacctgaa gccggtactg ccgaaactga ccgagcgtgc agaagccttc ctcaatacgg
     3361 aactgacctg ggatggtatc cagcaaccgc tgctggacca caaagtgaat ccgttcaagg
     3421 cactgtataa ccgcatcgat atgaagcagg ttgaagcact ggtggaagcg tctaaagaag
     3481 aagtgaaagc agctgccgcg ccagttactg gcccactggc agatgatccg attcaggaaa
     3541 ccatcacctt tgacgacttc gccaaagttg acctgcgcgt agcgctgatc gaaaacgcag
     3601 agtttgttga aggttctgac aaactgctgc gcctgacact ggatctcggc ggtgaaaaac
     3661 gcaatgtctt ctccggtatt cgttctgcct acccggatcc gcaggcactg attggtcgtc
     3721 acaccattat ggtggctaac ctggcaccac gtaaaatgcg ctttggaatc tccgaaggca
     3781 tggtgatggc tgccggtcca ggcgggaaag atatcttcct gctaagcccg gatgctggtg
     3841 ctaaaccggg tcatcaggtg aaataatccc ccttcaaggc gctgcatcga cagcgccttt
     3901 tctttataaa ttcctaaagt tgttttcttg cgattttgtc tctctctaac ccgcataaat
     3961 actggtagca tctgcattca actggataaa attacaggga tgcagaatga gacactttat
     4021 ctatcaggac gaaaaatcac ataaattctg ggcggtggag caacagggaa acgagttgca
     4081 tatcagttgg ggaaaagttg gcaccaaagg gcaaagtcag ataaaaagtt tttcagatgc
     4141 tgcggcagcg gaaaaagcgg aacttaagct gattgcggag aaggtgaaga aggggtatgt
     4201 ggagcaagcg aaggataatt ctttgcaacc ttcccaaacg gtaacgggct ctctcaaggt
     4261 agcggactta tccaccatta ttcaggaaca accctctttc gtagcagaaa cccgtgcgcc
     4321 tgacaaaaat acagatgctg ttttaccgtg gctggcgaaa gatattgctg tcgtttttcc
     4381 gcccgaagtt gtacacacca cgttaagtca tcgccgcttt cccggagttc ctgttcagca
     4441 agcagacaaa ttgacccaat tacgtcgctt agcctgtagt gtgtcgcaac gggataataa
     4501 aacagccaca tttgacttca gcgcctgttc tttagaatgg caaaacaccg tcgcccaggc
     4561 gatcagtcag atcgacggcc tgaaaacaac acagttacca tcaccagtaa tggctgtact
     4621 cacggcactt gaaatgaaat gcacaagata taaagtgcgt gaggatgtta tggatcagat
     4681 cgtccaggaa ggcggtctgg aatatgctac tgatgtaata atacaccttc aacagattga
     4741 tattgaatgg gattatgcga ataatgtcat tattattctg ccgtctggca ttgcacctag
     4801 ctacttggag caatattcca gatttgaatt acgcctacgt aaacatttat cactgacgga
     4861 agagtctctc tggcaaaaat gtgcacaaaa acttattgcc gcaattccac atattccaga
     4921 atggcggcaa ccattaattg ctttgttatt acccgaaaaa ccagaaattg cacatgaaat
     4981 tgcccagcgt ctactggggc aaaaaaaatt accctcgctt gagtggttaa aaatagtggc
     5041 gactgatgag cacattcttg cctcattaga aaaatatcac gaaccatatg ccatttttga
     5101 tgattactat tgtggtgcga tatggtcagc caccgtatta caggagcaag gtgttgcagc
     5161 cctgccccga tttgctccct atgccgcaag tgactactgc gccgatgtgt tgcgtcatat
     5221 caatcatccg ttcgcattga cactgcttat acgtgtagcc gggcaaacta aacgctgtca
     5281 cgatcggatg acgaaagcca ttgctgcgtt cccacatgca gcaatggcgg cactgacgga
     5341 acttcttggg caaaaagaag agaacagttg gcgcattatg ctaatgacaa tgcttatctc
     5401 acaaccagca ctggcagaac aggtcattcc ctggctctcg acacccgcag ttgccgtact
     5461 gaaatcatgc cagcaacaac tgacacagcc ctcaaaccat gccagcgccg atctactgcc
     5521 agccgtagta gtctcccctc cctggctttc gaaaaagaaa aaatcgccga ttccggtgct
     5581 ggatttagcg ccattaggca ttgagccaat ctgttatctg acagaagaaa tcagtaatca
     5641 acttttggcg aaatatatct ggtattcaaa acacatcacg gttagccatg aagaaagtac
     5701 taccaacctg ttggcaagga tgggttttca acgacggatc gctggtacat atattaaagc
     5761 tcccgaagcg gtagttgagg catggctaaa tgaagattat tcaaccttac taagtgaatt
     5821 taaggtgttt cattcaccta ccgggcatta ttggcagttg gggattttga caacattgcc
     5881 gctggagaaa gcagtaaaag catggaatgc ccttacccta tctccacata ccgataccga
     5941 atactccatg ttacattttg gactcaaagg gttacctggg ttagtaaact cacttgcacg
     6001 ctatccacaa gaagccttgc ccatcacgaa ttacttcgca gcgagtgagc tggctcctgc
     6061 cgtcgcccgt gccttcaaca aactgaaaac gctacgcgaa aacgcccgta gctggctgtt
     6121 gaaatacccg gaacatgccc ttaccggcct gctgcctgcg gcgctcggca aagccggtga
     6181 agcacaggat aacgcccgcg ctgccttgcg tatgcttacc gaaaacggtc atcagccatt
     6241 actgcaagaa atcgcccgac gttataacca gccggaagta accgatgcgg tgaacgctct
     6301 gcttgcgctc gatcccttag ataatcaccc gacaaaaatc cccactcttc cggcctttta
     6361 tcagccatcg ctctggacgc gcccggtatt aaaagcaaat gcccaatcac tgccagatag
     6421 cgccctcctc cacctcggtg aaatgctccg cttccctcag gaagaggctc tgtatccggg
     6481 attattgcag gtgaaagacg tctgttccgc cgactcactg gcgggatttg cctgggatct
     6541 gtttaccgcc tggcagaccg ctggcgcgcc gtcgaaagag agttgggcgt tcactgcgtt
     6601 aggcgttctc ggtaacgatg acaccgcccg caaactgacg ccattaatac gcgcctggcc
     6661 tggtgaatcc cagcataaac gcgccaccgt tgggttggat attctcgctg ctatcggtag
     6721 tgatatcgcc cttatgcagc ttaacggcat cgcccagaaa ctgaaattca aagcattaca
     6781 ggagcgggca aaagaaaaaa ttgccgacat tgccgagagc cgcgaactca cggtggcgga
     6841 gcttgaagat cggttagcac cggatctcgg tctggatgat aacggttcgc tgctgctgga
     6901 ttttggccca cggcagttca ccgtcagctt tgatgaaacc ttaaaaccgt ttgtgcgtga
     6961 tgtttccggc agccgcctga aagacctgcc caaaccgaac aaaagcgatg atgaaacgcg
     7021 ggcgaacgat gcggttaacc gctacaaatt gctgaaaaaa gatgcgcgta ccatcgccgc
     7081 ccagcaggta gcaaggctgg aatccgccat gtgcctgcgc cgccgctggt cgctggaaaa
     7141 cttccagctc ttcctagttg agcatccgct ggttcgtcac ttaacccgcc gtctgatttg
     7201 gggcgtttat agcgccgaaa accagctact ggcttgcttt cgcgtagcag aagataacag
     7261 ctccagcacc gctgacgatg atcttttcac cctgccggaa ggcgatatct ctatcggcac
     7321 tcctcacgtt ctggaaatat caccaacgga tgctgccgcc tttggtcagc tttttgccga
     7381 ctacgaactg ctaccaccgt tccgccagct cgaccgtaac agctacgccc tgacagaagc
     7441 cgagcgcaat gccagtgaac tgacccgctg ggcaggcaga aaatgcccga gtggtcgggt
     7501 catggggctg gcgaataaag gctggataaa gggcgaacca caggatggag gctggatcgg
     7561 atggatgatc aaacctttgg gtcgctggtc gttaatcatg gaaatcgatg aaggctttgc
     7621 ggtaggcatg tcgccagccg aactcagcgc tgagcagctc ttaagcaagc tgtggctatg
     7681 ggaaggcaaa gcagaaagat atggctgggg gagtaattca acacaggaag cgcagttctc
     7741 cgtaatcgat gccatcaccg ccagcgagct aattaacgat attgaagccc tgtttgaata
     7801 aggaaagagc atggacaagg aattaccgtg gctggcggat aacgcccaac tggaactgaa
     7861 atataaaaaa ggcaaaacgc cgctcagtca tcgtcgctgg ccgggcgaac cagtgtccgt
     7921 tatcactgga agtctcatcc agacattggg tgatgaattg ctacaaaaag ctgagaagaa
     7981 aaaaaacatt gtctggcgtt atgagaattt ttcactggag tggcagtccg ccatcacgca
     8041 ggccatcaac ttgatcggcg aacacaaacc ctcaatcccg gaccggacaa tggcggcgct
     8101 agcctgtatc gcgcaaaatg acagccaaca gttgctcgac gaaatcgtcc aacaagaggg
     8161 gctggaatat gcgactgagg tggtgattgc acgccagttt attgcgcggt gttatgagag
     8221 tgatcctctg gtagtgacat tgcagtatca ggacgaggat tatggctatg gttatcgctc
     8281 agaaacctat aacgaattcg atctccgact gcgtaagcat ctctctctgg cagaggaaag
     8341 ctgctggcag cgttgcgccg acaaactcat tgccgcacta ccaggaataa ccaaagttcg
     8401 ccgccctttt attgcgctga tcctcccgga aaaaccagaa atagccaatg agttggtagg
     8461 ccttgaatgc ccgcgaactc attttcattc taaggagtgg ttaaaagttg ttgctaatga
     8521 ccccacagcg gtgagaaaac tcgaacacta ctggagccag gatatattta gcgatcgaga
     8581 agccagctac atgtcgcatg aaaaccactt cggctacgcg gcctgcgccg cccttttgcg
     8641 cgaacaagga ctggcagcca ttccgcgcct cgcgatgtat gcccataaag aagattgcgg
     8701 cagtctgctg gtacaaatta accatccgca agtcatccgc accttgctac tggtggctga
     8761 taaaaacaaa cccagcctgc aacgtgtagc taaataccat aaaaacttcc cccatgcgac
     8821 gctcgccgca ctggcagaac tgctggcgtt aacagaacca ccagcccgcc ctggttatcc
     8881 aatcatcgaa gacaaaaagc tgcctgcaca gcaaaaagca cgcgatgaat actggcgtac
     8941 gctgttacaa acgctgatgg catcgcagcc acaactggca gcagaagtga tgccgtggtt
     9001 aagtactcaa ccccagtcag tgctgaagag ttatttatcg gcaccgccca aaccggttat
     9061 tgatggcacc gataacagca atctgccaga aatcctcgtt tcaccaccgt ggcgtagtaa
     9121 gaaaaaaatg acagctccac gtcttgattt ggcaccgctc gaattaactc cgcaagttta
     9181 ctggcaacca ggcgaacaag agaggcttgc cgccactgag cctgcccgtt atttcagcac
     9241 ggaatctctt gcgcaacgca tggaacaaaa aagtggacga gttgtattac aggaactggg
     9301 ttttggggat gatgtatggc tgtttctgaa ttatatactc cccggaaaac tggatgctgc
     9361 acgcaattca ctctttgttc agtggcatta ctaccagggg cgggttgaag agatcctgaa
     9421 tggctggaac tccccggaag cacaattagc agaacaggcg ctccgcagcg gtcacataga
     9481 agcgttaatt aacatatggg aaaatgacaa ctactcacat tatcgtccgg aaaagagtgt
     9541 ctggaacctg tatttattgg cacagttgcc gcgtgagatg gctttgacct tctggctgcg
     9601 tatcaatgag aaaaagcatc tgttcgcggg tgaggactat tttctcagta tcctcggatt
     9661 ggatgcgcta ccaggtctgc tgttggcttt ttcacatcgt ccaaaagaaa catttccgtt
     9721 aattttaaat tttggcgcaa cagaactggc gctgcctgtt gcccacgtct ggcgacgttt
     9781 cgcggcgcag cgtgatctgg ctcgccagtg gattttacaa tggccggaac atacggctag
     9841 tgcacttatc cctcttgtct ttaccaaacc cagcgataat agcgaagccg cattacttgc
     9901 cctgcgttta ctgtacgaac agggacatgg cgaattgcta caaaccgtgg caaaccgctg
     9961 gcagcgtaca gatgtatggt ctgccctgga gcagttgctt aaacagggtc caatggacat
    10021 ttacccggca cgcattccaa aagcccctga tttctggcat ccgcaaatgt ggtccaggcc
    10081 gcgccttatc actaataatc aaactgttac caatgacgct ctggaaatta tcggcgaaat
    10141 gctgcgcttt acccaggggg gacgttttta tagcgggctg gaacaactga aaacgttctg
    10201 ccagccacaa acgctggcag cttttgcctg ggatctcttc actgcgtggc aacaagctgg
    10261 tgcccccgca aaagacaact gggcatttct ggcgttaagt ctctttggtg acgaaagcac
    10321 ggcacgggat ctgacgacac agatcctcgc ctggccacaa gaaggcaaat ctgcccgtgc
    10381 tgtcagcggc ctgaacatcc ttaccctgat gaataatgat atggcgctga tacagctgca
    10441 tcatatatcg caacgggcta aatcccgccc cttacgtgat aacgcggcgg aatttcttca
    10501 ggtggtcgca gaaaatcgcg ggctaagcca ggaagagcta gcggacagat tagtcccaac
    10561 cctgggcctt gatgatccgc aggcgttgag ttttgatttt ggtccccggc agtttaccgt
    10621 tcgcttcgat gaaaatctca acccggttat ctttgatcag caaaacgttc gccagaaaag
    10681 cgttccccgt ttgcgcgccg atgacgatca actgaaagcg cccgaggcac tggcccgact
    10741 aaaagggcta aaaaaagatg ctactcaggt gagcaaaaac ctgctcccgc gtcttgaaac
    10801 ggccctacgt accacccgac gctggtcgct ggcagatttt cattctctgt ttgttaatca
    10861 tccctttacc cgtctggtta cccagcgatt aatatggggg gtttatccgg caaatgaacc
    10921 gcgttgttta ctcaaagcct ttcgtgtggc cgcagagggg gagttctgca atgcgcaaga
    10981 tgagccaatt gacctgcctg cggacgctct gattggcatt gcccacccgt tagaaatgac
    11041 agcagaaatg cgcagtgaat ttgcacagct ttttgccgat tacgaaatta tgccgccttt
    11101 tcgccagttg tcgcgccgca cggtgctgct cacacctgac gagtcaacca gtaacagcct
    11161 gactcgctgg gaaggtaaat ccgctaccgt tgggcaactt atgggaatgc gatacaaagg
    11221 ctgggagtca ggctatgagg acgcatttgt ctataacctg ggtgagtacc ggctggtcct
    11281 taagttttca cccggtttta accactacaa tgttgatagc aaagcgctaa tgagcttccg
    11341 ttctcttcga gtgtaccgtg acaataaatc cgtcactttt gccgaacttg atgtgtttga
    11401 tttgagtgag gcgttaagcg cacctgacgt cattttccat taaaacacag cgggcagtga
    11461 tgcaactgcc cgttatcaac acgacttacc agcggggaaa gcgatgatcg tgcaaaaaga
    11521 gctggttgct atttacgatt atgaggtccc tgtacctgaa gatccgtttt ccttcagact
    11581 tgagatccat aaatactctg aattatttac aggttccgtc tatcgactgg agcgattccg
    11641 gctacgtcca acatttcatc aacgtgatcg agaagatgct gacccgctaa taaatgatgc
    11701 gttgatttat ataagagatg agtgtattga tgagcggaaa ttacgaggtg aatcacctga
    11761 aactgtaata gcaattttta atcgtgaact acagaatata ttcaaccaag aaatagaata
    11821 atatactcta aataattcaa attggtccga tccggcgcaa cgtcccaatg gcctggatta
    11881 taaatctcat tatcttaatt gcaacggggt ccagccgtgg ttataatttg ttcgttggaa
    11941 cacgatgttg cacaggctgt ggtgttaggc ctgaaaatag taaataaaag aacctgcctc
    12001 accagcaggt ttttttattt actgtgatct gctttccaga tatttttcgc tcaaacaact
    12061 aatgcgccaa acatttattg cgcgtaaaat atcgtttatt tcattaatac atttcaggga
    12121 tgaatatatg tcaccgcaga ataatcatct gcagcgtccg cctgctgctg tgttatacgc
    12181 cgatgaactg gcaaaattaa aacaaaatga taacgcacct tgcccgcccg gttggcagtt
    12241 aagtttgcct gcggcccgtg cttttatcct tggcgacgaa gcgcaaaata tcagccgtaa
    12301 agtggtgatt agcccctccg ctgtcgaacg tatgttagtc actcttgcta ccggccgcgg
    12361 tttgatgttg gtgggggaac cgggtaccgc aaaatctctt ctttctgaat tactggcaac
    12421 cgctattagc ggcgatgccg ggttaaccat ccagggcggg gcatctacca ccgaagatca
    12481 aatcaagtat ggctggaact acgccctgct tatcaatcat ggtccgtcaa cggaagcgct
    12541 ggtccctgcc ccgctttatc agggaatgcg cgacggcaaa atcgtccgtt tcgaagagat
    12601 aacgcgtaca ccgctggaag tgcaggactg tctgttaggt atgctttctg acagggtgat
    12661 gacggtgccg gaactcactg gtgaagccag tcagctctat gcgcgagaag gctttaatat
    12721 tattgccact gccaataccc gcgatcgcgg cgtcaatgaa atgagtgctg cgctaaagcg
    12781 tcgcttcgat tttgaaacgg tttttccgat tatggatttc gcccaggagt tggaactggt
    12841 cgccagtgct tcggcgcgtt tgctggccca tagcggtatt ccgcataaag tgcctgacgc
    12901 ggtactggaa ttactggtcc gcaccttccg cgatctgcgt gccaacggcg aaaagaaaac
    12961 gtcaatggat acactgacag cgattatgtc caccgctgaa gccgtgaacg tggcccatgc
    13021 tgtgggcgtc cgcgcctggt ttttagcgaa tcgcgcgggg gagcctgccg acctggtgga
    13081 atgtattgcc ggaaccatcg tcaaagataa cgaagaagat cgcgcgcgtc tgcgccgtta
    13141 cttcgaacag cgtgtcgcca cacataaaga agctcactgg caggcttatt atcaagcccg
    13201 ccaccgcctg ccgtgaggaa agatgcatga gcgagccgtt aattgtcggc atccggcatc
    13261 atagtccggc ctgcgcccgg ctggtgaaat cgttaatcga aagccagcgg ccacgatacg
    13321 tgttgattga aggcccggct gattttaatg accgggtaga cgaactgttt ttagcccacc
    13381 agcttccggt agctatttac agttattgcc agtatcagga cggtgcagcc cccgggcgtg
    13441 gtgcctggac gccatttgct gaattttcgc cggagtggca ggcgctacaa gccgcacgtc
    13501 gcattcaggc acaaacttac ttcatcgatt tgccttgctg ggcgcagagt gaagaagagg
    13561 acgattcgcc tgatacgcaa gatgaaagcc aggccttact gctgcgtgcc acccgcatgg
    13621 ataacagcga taccctgtgg gatcacttgt tcgaagatga aagccagcaa actgcattac
    13681 cctctgcgct ggcgcactat tttgcccaac tgcggggcga cgcctccggc gatgcgctca
    13741 atcgtcagcg cgaagccttt atggcccgct ggattggatg ggcgatgcag caaaataatg
    13801 gcgacgtgtt agttgtctgc ggtggctggc acgctccggc actggcaaag atgtggcgcg
    13861 aatgccctca gaaaattaac aagccagaat tgccctcgct ggcagatgcc gttacaggtt
    13921 gttatctcac accctacagt gaaaagcgcc ttgatgtgct ggcaggatac ctttcaggaa
    13981 tgcctgcccc ggtatggcaa aactggtgct ggcagtgggg cttgcagaag gccggtgaac
    14041 aactgctaaa aactatcctt acccgtttgc gccagcacaa attgcccgct tctaccgcgg
    14101 atatggctgc cgctcatctg catgcgatgg cgctggcaca gttgcgcggt catacactac
    14161 cgttacgcac tgactggctg gatgccatag caggctcgct gattaaagaa gccctgaacg
    14221 cgccgttgcc gtggagctat cgcggcgtta ttcatcccga taccgatccg attctgctaa
    14281 cgttgataga cacattagcg ggtgacggat tcggtaaact tgccccttct acgccacaac
    14341 cgcctctgcc aaaagatgtc acctgcgaac tggaacgtac cgcaatctcc cttccggcgg
    14401 agcttacctt aaatcgcttt acccccgatg ggctggcgca aagtcaggtg ttacatcggc
    14461 tggcaatact ggagatccct gggattgtac gccagcaggg aagtacactg acacttgcag
    14521 gcaacggtga agaacgctgg aaattaaccc gcccgcttag ccagcatgcg gcattgattg
    14581 aggccgcctg ttttggtgcc acactccagg aagccgcacg caataaatta gaagccgata
    14641 tgctggacgc gggcggaatc ggcagtatca ccacatgtct tagccaggcg gcgttagcgg
    14701 gtctggcgtc cttcagtcaa caattactgg agcaactcac actattaatc gcccaggaaa
    14761 atcaatttgc cgaaatgggc caggcgctgg aagtgcttta tgccttatgg cggctggatg
    14821 aaattagcgg tatgcaaggc gcgcagatat tacaaacgac gttatgcgcg actatcgatc
    14881 gcacgctgtg gctgtgtgaa tctaacggca gaccggatga aaaggagttt cacgctcacc
    14941 tgcatagctg gcaagcgctt tgccatattc tgcgcgatct acatagcggc gttaatttac
    15001 ccggcgtttc tctttctgcg gcggtagcct tactggagcg acgcagtcag gcaattcatg
    15061 ccccggcgct ggatcgcggc gcggctcttg gcgcactgat gcgtctggaa catcccaacg
    15121 ccagtgccga agcggcgctg acgatgctgg cgcagttatc cccggcacaa tctggtgagg
    15181 cgctgcacgg tttgctggcg ctggcccgcc atcaactggc ctgtcagccg gcatttatcg
    15241 ccggtttcag cagtcattta aatcaactga gtgaagccga ttttattaac gccctgcccg
    15301 atttacgcgc ggcgatggcc tggctaccac cacgagaacg cgggacgctg gcgcatcagg
    15361 tgcttgagca ttatcaactg gcgcaacttc ccgtttcggc gctgcaaatg ccgttgcatt
    15421 gtccaccaca ggccattgca catcatcaac aactcgaaca gcaggcactg gcatcgctgc
    15481 aaaactgggg agttttccat gtctgaactg aacgatcttc tgaccacccg tgagctacaa
    15541 cgctggcgat taattcttgg cgaagcggca gaaacgacgc tttgtgggct ggatgacaac
    15601 gcccggcaga tagaccacgc gctggagtgg ctgtatgggc gcgatcctga acggctccag
    15661 cgtggtgaac gttccggtgg attaggtggc tcaaatctca ccacccctga gtggatcaac
    15721 agtattcaca cgctgtttcc gcagcaggtg attgagcggc tggaaagcga tgccgtgctg
    15781 cgctacggca ttgaagatgt ggtgacgaat ctcgacgtgc tggaacgtat gcagccttct
    15841 gaaagcctgc tacgcgctgt tttgcacacc aaacatctga tgaaccccga agtactggct
    15901 gccgcccgcc ggatagtgtg ccaggttgtt gaagaaatta tggctcgact ggcaaaggaa
    15961 gttcgtcagg ctttttctgg tgtccgcgat cgccgtcgcc gttcatttat tccactggcg
    16021 cgaaactttg atttcaaaag tactctgcgc gccaacctgc aacactggca cccgcaacac
    16081 ggcaagttgt atatcgaatc cccccgcttt aacagccgca ttaaacgcca aagcgaacaa
    16141 tggcaactgg tcttactggt tgatcaaagc ggatcgatgg tcgattcggt gatccactct
    16201 gcggtgatgg cggcctgttt gtggcagtta cccggcattc gtacccatct ggtggcgttt
    16261 gacacaagcg tcgttgatct cacggcagac gttgccgatc cggtagagtt attaatgaaa
    16321 gtacagttgg gcggcgggac caatatcgcc agtgccgtgg agtatggtcg gcaacttatt
    16381 gaacaaccag cgaaaagcgt cattatcctc gtgagcgatt tttacgaagg gggttcatca
    16441 tcattactga cgcatcaggt gaaaaagtgt gtccagagcg gcatcaaagt gctgggactg
    16501 gcagcgctcg atagcaccgc aacaccttgc tatgaccgcg atacggccca ggcgctggtt
    16561 aatgtcggcg cacaaatagc cgccatgacg ccgggcgagc tggcatcatg gcttgcggag
    16621 aatcttcagt catgaattca ctacgtccgg aattattaga actgacaccg caggccctga
    16681 cggcgttaag caatgccggt tttgttaagc gcagtcttaa ggaactggaa aatggcaacg
    16741 tcccggagat cagccatgag aacgacgctt taatcgccac cttcagtgac ggtgtccgta
    16801 cccagctggc gaacggccag gcactgaaag aggctcagtg cagttgcggg gccaacggta
    16861 tgtgccgtca tcgcgtgatg ctggtgttaa gttatcaacg actttgtgcc accactcagt
    16921 ctacggaaaa agaagaagag tgggatccgg caatctggct ggaagaactg gctacccttc
    16981 ccgatgctac ccgcaaacgc gcacaggcgc tggtcgctaa aggcatcacc attgagttgt
    17041 tctgtgcgcc gggtgaaatt ccctctgccc gcttaccgat gagcgatgtg cgtttttatt
    17101 cccgcagcag tattcgtttc gcccgttgtg attgtattga cggcacactt tgcgaacatg
    17161 tcgtactggc ggtacaggcc ttcgtcgagg ccaaagcgca gcaagcagaa tttaaccatt
    17221 taatctggca gatgcgcagc gaacacgtca catcatctga cgatccgttt gccagcgaag
    17281 aaggcaacgc gtgtcgtcaa tatgttcagc aattaagcca gacattatgg cttggcggca
    17341 tcagccagcc gctcatccat tacgaggcag cattcaaccg cgcattgcag gcggcggaac
    17401 gctgtaactg gcgctgggtg agtgaatcgc tacggcaact gcgcgccagc gttgatgcct
    17461 tccacgcccg cgccagccac tatcatgccg gagaatgctt acgtcagctt gcggcattaa
    17521 acagtcgatt aaattgcgca caagagatgg cgcggcgcga cagtgttggt gaagttcatc
    17581 cgatgccgtg gcgcacggtc gttggcgctg gcattgccgg agaagcaaag cttgatcatc
    17641 tgcggctggt gtctttaggt atgcgttgct ggcaggatat tgagcagtat ggtttacgca
    17701 tctggtttac cgatcccgac accggcagta ttttgcatct ttcgcgtagt tggcagagaa
    17761 gcgaacagga aaactcaccg gccgcgacgc gtcggctgtt tagttttcag gctggcgcac
    17821 tggcgggcgg gcaaattgtt tcacaagcag caaaacgcag tgccgatggc gagctgctgt
    17881 tagctacccg caaccgctta agcagcgttg tgccgctgtc gcctgatgcc tggcaaatgt
    17941 tgagcgcgcc gttacgccag ccgggcattg tggctttgcg tgaatattta cgccagcgtc
    18001 cccccgcctg catacgacca ctcaaccagg tcgataactt atttattctg ccggtagcgg
    18061 agtgtatttc gctcggttgg gatagcagcc gccagacgct ggatgcgcag gtaatcagcg
    18121 gcgaagggga agataatgtg ctgacgttat cccttccagc atctgcctgt tctccttttg
    18181 ccgttgaacg catggcagct ctgttacaac aaacagacga ccccgtgagt ctggtttctg
    18241 gctttgtcag ttttgttgat gggcaattga cactggaacc acagataatg atgacaaaga
    18301 ctcgtgcctg ggcgctggac gcagaaactg cgcctgtggc tccgctacct tctgccagcg
    18361 ttttgcctgt gccgtctacc gctcatcagt tgctgatgcg ctgccaggcg ttacttattc
    18421 aactgctcca taacggctgg cgctatcagg aacagagtgc tattggtcag gcagagttgc
    18481 tggcgaatga cctcaccgcg gtcggttttt atcggctggc acatgtgttg gcacaatttc
    18541 gtaatacaga aagcgatgca cgggtagaag caatgaataa cggtgttttg ctttgcgaac
    18601 aattattccc catgcttcag caacaaggat gaaatagtgc tttttactaa gagttctact
    18661 ccagttccgg actgctcacg ccacagtatt aggcatatcc tatatagccc ctggtgagag
    18721 tcaccagttc cttgattaaa taaaatggag ttttacatga aggctttcaa taagctgttt
    18781 tcccttgttg ttgcatctgt tctggttttc tctcttgctg gctgcggtga caaagaagaa
    18841 tcgaaaaaat tcagcgccaa tctgaacggt actgaaattg ccattaccta tgtctacaaa
    18901 ggtgacaagg tgcttaagca atcttctgaa accaaaattc aatttgcctc cattggtgca
    18961 accaccaaag aagacgctgc caggacactt gagccgttaa gcgccaaata caaaaacatc
    19021 gcgggtgttg aagaaaaatt aacctatacc gatacttacg cgcaggaaaa cgtgactatc
    19081 gatatggaaa aagtggattt caaagccctg cagggtattt caggaatcaa cgtttctgct
    19141 gaagatgcca aaaaaggtat cactatggcg caaatggaac tggtgatgaa agccgctggt
    19201 tttaaagaag tgaaataatc tgtcggcggc catgtttttg catggccgcc atacccgtct
    19261 tagcttttct tcacatgctg gcgcgctgcc agtccacgca gaaaataacg taaaaattga
    19321 tcgccgcatt cgcggaagtt tttatgatcc ggtgcgcgca tcatcgctgt aatttccggc
    19381 atcgaaacgc ggaactgctg ttcggtgagg atcgccagaa tgtcatcggt tttcagcgaa
    19441 aacgcgatgc gtaatttttt cagcacgatg ttgttattaa tgcgacgttc cggctccagt
    19501 gccggagcag actcatcctt gccgcgtttt tcataaatca ggccattgag gaatgacgac
    19561 aaaacaatgt ccggacaacg ctgaaaaccc tcttcgtctt ctttacgtag ccagacggcg
    19621 atctgttccg cggtggcttc gacattaccc agcgccagaa tacgcaccag gtcattatta
    19681 ttggctttca aaatgtagcg cacgctgcgc agaatatcgt tacttagcat gaggccttca
    19741 ggtgttgatg aggcaaaaag ccattttagc agtcttttac aggccaatcg cctcttttaa
    19801 gcttttcaga taacggcggc tgaccggcac ggttaagcca ttacgcaaaa tcaactcggc
    19861 ctggccgtta tcttccagac gaatctcctg taaatgcgcg aggttaacca gatactgacg
    19921 atggcagcgc agtagtggtg tacgactttc cagggtacgt aatgtcaatt cggtaaagcc
    19981 ctctttccct tcgtggctgg taacgtagac accgctcatc cgactgctga caaatgccac
    20041 atctttcatt tgcagtaaat aaatccgact atgccccgta caagggataa atttcagcgc
    20101 ctgttgattt tccggtaaca gcgaaacatc ctgcttactg cgttcctgac gcaaacgcgc
    20161 cagcgttttc tccagtcgcg cttcatcaat tggcttcagc agatagtcaa aggcatgttc
    20221 ttcaaaggct ttaatagcgt attcgtcaaa cgcggtgaga aaaacgatat acggtcgatg
    20281 ttcggggtcg agcatcccca ccatttccag accactgatg cgcggcatct ggatatcgag
    20341 aaacagcaca tccgggcgca gtttatgcac cgcgccgatc ccttctacgg cgttagaaca
    20401 ctctccaacg atttcaatat cgctctgctc ctgcaaaaat acgcgcaggt tctcccgtgc
    20461 aagcggttca tcatcgacaa ttaagacttt aatcatgcct cgtccctcca tggtagtcgt
    20521 aacgttattc gggtgtaaca atcaggctca caggcgacgc ttatcccata gtcatcgcca
    20581 aaccgttcac gtaaacgctt atccaccaga ttcatcccca gcccactggc attggttacc
    20641 ggttgataca aaccggcatt gtcttcgatc tccagcatca aatgttgccc ctcacgtcgg
    20701 gcgctgattg ccactcgccc tgtatccagc agttgtgatg tcccatgttt aatggcgttt
    20761 tccactatcg gttgcagggt aaacgcgggc aattgctgct gggataattc ttgcggaata
    20821 gcaatgttga cctgcaaccg cgactggaag cgcgcctttt caatttgcag ataagcgttc
    20881 acatgttcaa tttcgtctgc gagagtaaca aactccgaag gccgctttaa gtttttgcgg
    20941 aaaaaagtgg aaagatactg gaccagctgg ctggcctgtt cgctgtcgcg gcggatcacc
    21001 gctttaatgg tgttaagcgc attaaacaaa aaatgggggt tcacctgggc gtgaagcagt
    21061 ttgatctctg actgggtgag catcgctttt tgccgctcat attgcccggc gaggatctgc
    21121 gccgaaagca gttgcgcaat cccctcgccc agcgtgcggt tgattgaact gaataaacgg
    21181 tttttggctt catacaattt gatggtgccc atcacccgct gattttcgcc acgcaacgga
    21241 attaccagcg tcgaccccag tttgcattgc ggatgcaaag agcaacgata aggtacttcg
    21301 ttgccatcag cgtagaccac ttcaccggtt tcaatcgctt ttaaggtata agtcgaagaa
    21361 atcggtttgc cgggtaaatg gtggtcgtca ccaattccgg taaaggccag caatttctct
    21421 cgatcggtaa tcgcgactgc accaatatcc agctcctgat acagcacctg tgccactttc
    21481 atgctgttca cttcgttaaa cccctggcgc aaaatgcctt ccgtcgaggc cgccactttc
    21541 agcgcagtgg cagaaaaagc cgaagtgtat ttttcaaaca tcgcgcgttt atcgagcaat
    21601 atacgcataa acaacgccgc gccgacggta ttggtgacca tcattggcgc agcaatatta
    21661 ctcaccagac gcaccgcatc ttcataaggt cgggcgatcg ccaggataat caacatttgc
    21721 accatttcag cgacgaacgt gacggcaccg gcggtaatgg ggttaaagac tttatcagtg
    21781 cgcccgcgac ggatcaggat gctgtgtacc aggccgccga gcaatccttc aacgatggtc
    21841 gagatcatgc aacttagcgc ggtcatgccc cccatcgaat atcgatgtaa cccgccggtc
    21901 agaccaacca gcccaccgac gaccggaccg ccgagtaagc cgcccattac cgcacctatc
    21961 gcacgggtat tggcaataga atcgtcaatg tgcaacccaa accaggtgcc catgatgcag
    22021 aagatggaaa agacgatgta gcagagaaat ttatgcggca gacgaaccgt gacctgcatt
    22081 aacggtatga ataatggcgt tttactcatt aaccacgcaa tgactaaaaa aacgcacatc
    22141 tgctgaagca gcagcaacac cagattaaaa tcgtacatac ccgcaaacca cacttccctt
    22201 taaaacgcgt aacatacatt gcttgcgttt aactttcttt gaactcttgc agaaaaatga
    22261 gaattcgtga gtacgatcac tcaaaatcgc ctggcaaaaa taaaatcacc ctatagatgc
    22321 acaaaaaacg ggcaaaacta cctggttcgc aaaactgcgt ctaaagttaa accgggacct
    22381 cgcgagcaag ggtgagacga tggcgcttta cacaattggt gaagtggcgt tgctttgtga
    22441 tattaaccct gtcacgttac gcgcgtggca gaggcgttac ggattgctga aaccacaacg
    22501 gacagacggc ggtcatcggc tgttcaacga tgccgatatt gaccggatcc gcgagatcaa
    22561 acgctggatc gacaacggcg tgcaggtcag caaagtaaaa atgctgctca gtaatgaaaa
    22621 tgttgatgtg cagaacggct ggcgcgatca gcaagaaaca ttactgactt acctgcaaag
    22681 cggcaatctg catagcctgc gaacgtggat caaagagcgc ggtcaggatt accccgccca
    22741 gacactcacc acacatctgt ttattcctct gcgccgacgg cttcagtgcc aacaaccgac
    22801 tctccaggcg ctgctggcga tcctcgacgg cgtactgatc aactacatct ctatttgcct
    22861 ggcttcggcg cgcaaaaaac agggtaaaga tgcactggtg gttggctgga atattcaaga
    22921 taccactcgt ctgtggctgg agggctggat tgccagtcaa caaggatggc gcattgatgt
    22981 cctcgcccac tcgctcaatc aactacgccc tgaactgttc gaaggccgta cattgctggt
    23041 atggtgcggt gataatcgaa cctccgccca acagcagcaa ctcaccagtt ggcaggaaca
    23101 aggccatgat attttcccac tcggcattta atgattcgtt aacaaatgcg ctttactgta
    23161 caatcctttc gttaacataa ggagtgcatt atgcgcatag ctaaaattgg ggtcatcgcc
    23221 ctgttcctgt ttatggcgtt aggcggaatt ggtggcgtca tgctcgcagg ttataccttt
    23281 attttgcgtg ctggctgaac gcctgcacca gcctttcaaa caggcggtct gcgatgatcg
    23341 ccgccagtgc caccagtaac gctccctgga tcacatacgc ggtattaaat ccgctaagtc
    23401 cgatgatgat gggcgtaccc agcgtgttgg cccctaccgt tgaggcgatc gtcgccgtgc
    23461 caatgttgat aatcaccgaa gttcgtacac ccgccagaat caccggagct gccagcggta
    23521 gctcgacctt acgcagtcgc tgactacagc tcattcccat gcctttggca acttcggtca
    23581 cgctggcatc aatcgatccc agcccggcaa gtgtcgcctg caggacgggc agcacaccgt
    23641 aaaggatcaa ggcgataatc gccggttgct gaccaaagcc gatcaccgga acggcaatcg
    23701 ccagcactgc aacgggcgga aaagtctgtc caacggcggc aatagtttcc accagtggac
    23761 gaaattccgc gccccaaggg cgagtgacag caattccggc accagtacca atgatcaccg
    23821 caaacaaact cgaaattccc accagccaga aatgagccag tgccagagct gcaaaacttt
    23881 cttgctgata aacgggtcgt ggcagttgtg ggaacaaggc agcaaacagc ggctggctgt
    23941 aaggcagcca gaaaatcagc gccacaaaca gagcaatgag ccagaacagc ggatcgcgca
    24001 acatcttcat acgcttacgc ctccaccagc agatcctgaa aatgcagcgt gccgcaaggc
    24061 tggccctgcg tgttcaccac cggcagcacc tcgcatcccc gcgcgacaaa cagggagagc
    24121 gcatcgcgta gcgtcatctc ttctgccagt gcctcacctt ctgcccgttc ttcgcgacgc
    24181 acgtaatccg ccacactacg taacgaaagc aggcggacac ccagttcact atgcccaaaa
    24241 aactggcgga caaaatcatt cgccggacga gtcagcatcg tcagcggatt gccctgctgc
    24301 accacttcac cgtgacccat caataccaga tgttctgcca gccgtagcgc ctcatcaata
    24361 tcatgagtga ccagcacaat agtgcgcccc agcaaacggt gaatgcgcgt catctcttgt
    24421 tgcaacgcgc cgcgcgttac cgggtccagt gcgccaaaag gttcatccat cagtaagact
    24481 tgcggatcgg cagccagtgc gcgcgccact cccacacgtt gctgctgacc accggaaagc
    24541 tgatgtggat aacgctcacg caaatttggc tccagcccca gtagcgccat taattcgtcg
    24601 atacgatcgt cgatccgcgc ccgtgaccat ttttgtaatt gcggcacggt agcgatgttt
    24661 tgcgccacgc tccagtgggg aaacaggcca atagattgaa tggcatagcc catccggcgg
    24721 cgcaactcca gcactggcag cgagcgaatt tcttctccgg caaagcggat cacgccactg
    24781 tcatgctcca ccaggcggtt aatcattttc agagtggtgg atttaccgga gcctgatgta
    24841 ccaatcagca ccgaaaaact cccctcctga aaattgagat tgagatcgtt aacggctttt
    24901 tgtgcgccga acagtttgct gacatggcta aattcaatca ttacgtttca ccttcagcag
    24961 tgcgataagc aaatcgaaca gcgcatcgat cagcaccgcc agaacaatta ccaggatcac
    25021 ccccagcaac actaaatcaa tggcactgct tagcagcccc tggaaaacca gcgcaccaaa
    25081 accgcctgca ccgattaacg ccgcaatcac cgccatgccg acggtttgca ccatcaccac
    25141 ccgcaggcta cgcagaaata ccggtaacgc cagcggtaac tgaacatgca ggaatcgctg
    25201 cgccccgctc atgcccatcg ctctggcgct ctccagcaca tcgcgcggga tctggttcaa
    25261 accgactacc acaccgcgca ccagcggcag caaggcatag agcaccagcg caatcagtgc
    25321 gggtgtcatt ccggttcctg ctatgccgag cttacccagc cacggaaagg ccgtaaccag
    25381 tgcggcaagc ggcgcaatca gcaggccaaa gagcgccacc gaaggcacgg tctgaatgac
    25441 attgagcaga gaaaaaatcg ccccctgccg cgcggtggaa aagtagcacc agatgcccaa
    25501 cggcacacca atcactaacg caggcagcac cgcaccaaac agcaacgtca gatgttgtgc
    25561 cagcgcgtcg tcaaacacat cctgacggtt ggcgtattct ttcattagtg agagatcgtt
    25621 aagcgcgccg gaatacagca accacagcgg aatgatggca atctgcatat gcaacaacca
    25681 gcgccacagc ggatgcgtgg agattcggcg gatggcatcg ctacaggcca gcaatgccag
    25741 cgccgcagcc agccagaaac cactgccgag gctggtacgc gccagcgcac tgccattttg
    25801 tgccagttgt gtcgccgcct ttcctgctcc ccacaccagc aatacgaaga cgaattgcgc
    25861 cagaatgagt gcaaaaatgc tgcctttttt agcgggaata aaacaggctg tcatccaggc
    25921 gcaaccaacg cccaccagca tccagatcgt ttgcggccac agctgccaga gatgacgccc
    25981 ctcacccgaa actaaacgat taggcgcgta actgataaac ggcagcgccg ctgcgattgc
    26041 cgtcagcaac agcagcagcg ccagaacagg attaatacgg aaataagtca cgagaaatta
    26101 cttcgtccac cctttttgtt tcaggtagtc ggcagccact tttttggcat ccagtccttc
    26161 cacagcaatg ctggcattca gttgctgcaa tgttttttca tcgaggctgg cgaagactgg
    26221 ctgtagccac tgtgccattt gcggatactc cttcaacacc gattcacgaa ccactggtgc
    26281 aggcgcgtag ataggttgca cgccttgcgg atcgcttaaa gtttgcagcc ccagcgccgc
    26341 gaccgggccg tcagtgccgt aagccatcgc cgcattaacg ccagaggttt gctgggcagc
    26401 ggctttgatc gtcaccgccg tgtcaccacc ggccagtgac agcaactgat cctgactgag
    26461 cttaaagcca taggcttttt caaacgcggg taaggcatcg gcgcgttcga taaactctgc
    26521 cgaggctgcc agtttaaagg tgccgccctc tttcagataa cgactcaggt cggcaagcga
    26581 agtgagtttg tttttctctg ccacatcctg acgcacggcg atggtccagg tgttatttgc
    26641 aggcgcgggc gtcagccaga ttaacttgtt ttgctctgca tcgagttttt tgactttctc
    26701 atagccttgc tgcgcgtttt tccatgccgt atcgttttca tctttaaaga aaaacgcgcc
    26761 attgccagta tattccggat agatatccag ctcgccagaa gtgatcgccc cgcgcactac
    26821 gggagtcgtt cccagctgca ctttatttac cgtaggaacg ccgtgacttt ccagcacctg
    26881 caaaatgata ttgccgagca gcgcgccttc cgtatcgatt tttgaaccaa ccttaacggg
    26941 ggaagccgcc tgtagcggca ggctcacggc tgccaacaaa accagtgaac ctgcccagac
    27001 ctttgagagt ggcatgatgc tttcctcatt cttttactgt tgttttcagc gaattaagag
    27061 aaaagcatag ttgataatgg cggagttagc ctgagcggac ggattcagtt gcagaatcag
    27121 ataaatactt aaaaggcata ttttgcccgg cgtttcttgc cggatgcggc gtaaacgcct
    27181 tatccggcct acaaacatcg aaaattacag caactcaaac tcgcctttct taacgcgtgc
    27241 ggaatcagtg ccgatgaaga cattgaactt gccaggctcg gcgtcatatt tcatctgttg
    27301 attccagaac ttcagcgcct caatatcgat tgggaagctg acggtctgag tttcgcccgg
    27361 tttcagggtg attttctcaa agcctttcag ctgcttcacc gggcgactca tggaagccgt
    27421 cacatcctgc aagtacatct gtactaccgt ggccccttcg cgtttaccgg tgttcgtcac
    27481 ctgcacgctg gccgtcactt tgccgtcacg cttcatggtc ggcgcagaaa gtttcacatc
    27541 agagacggtg aaagtggtgt agctcagacc atagccgaac ggataaagcg ccccgttagc
    27601 ttcatcaaaa taacgcgaag tgtatttgtt cggcttgtcg gcattatacg gacgcccggt
    27661 attcagatgg ctgtagtaca ccgggatctg cccgacagaa cgcgggaagg acatcggtag
    27721 cttaccggac gggttgtaat cgccaaacaa tacatcggca atcgcattcc ccccttccgt
    27781 acccgcaaac caggtttcca gaatcgcatc agcctgctga tcttctttca ccagcgccag
    27841 cggacgcccg ttcatcagca ccagcaccag cggtttaccg gtggctttca gcgccgcaat
    27901 caagtcacgt tggctttgcg gaatagtaat atcggtacgg ctggaggcct cgtgcgccat
    27961 cccctgtgct tcaccgacca cagccaccac cacatcagat tgtttcgcgg tttgcactgc
    28021 ttcatcaatc atctcttgcg gcgaacgcgg atcaactttg accgcctctt catactgatt
    28081 caggaaatcg ataatgcctt tgtcactggt aacgttcgcc cctttggcat acagcacttt
    28141 accgttttca ccgacggcgt ttttaatccc tgttagcaca gtaacagatt gatcggcgac
    28201 acctgccgcc gaccagcttc ccatcacgtc acgcttgctg tctgccagcg ggccaaccac
    28261 cgcaatggtt gcagattttt tcagcggcag cgtttcgaga cggtttttca gcaacaccag
    28321 gctttcgcgc gccacttcac gcgcttcttt acggtgcagg cggctttcgg cattggtatc
    28381 caccgggtca gactctttcg gccccaaatg gctgtatggg tcgttaaaca atcccatatc
    28441 atatttaacg ttcagtacat ggcgggcagc atcgtccagc tcttccatcg tcactttgcc
    28501 ggatttgatc aacccaggca gatacttcga gtaatactcg tcgctcatgc tcatgttgat
    28561 tccggacttc agcgccacgc gtaccgcatc ctctgggtct gccgccgtgc catgtttaat
    28621 cagctctttg attgcgccgt gatcggaaac ggtgatgcct ttaaagcccc actggtcgcg
    28681 cagaacatct ttcagcagcc aggagtcgga ggtggctggc gtgccgttca gcgagttcag
    28741 cgccaccatc accgcaccgc tgcctgcatc cagccccgct ttgtacggcg gcatatagtc
    28801 gttaaacagg cgctgcggac tcatatcgac ggtgttgtac tctttaccgc cttctaccgc
    28861 gccgtatgcg gcaaagtgtt tgacgctggt catcaccgag tagcgatctg ccgggctttt
    28921 accctgcatc gcttccacca tggttttgcc cattattgag gtgagatacg tatcttcgcc
    28981 aaaaccttcg gaagcacgtc cccagcgcgg atcgcgcgag acatcgacca tcggtgccca
    29041 ggtcatattc aggccatcat ctgccgcttc ataagcagag acacgcccga ctgttttcac
    29101 cgcatcgagg ttaaaagacg aagccagacc gagactaatc gggaacaccg tgcgctgacc
    29161 gtgcagcacg tcgtaagcaa agaaaagagg aattttcagg cggcttaatt ccatcacctg
    29221 atcctgcatg gcgcggatat cctgacgggt tacagtgttg aaaatcgccc caacctgacc
    29281 gtctttgatc atctcgcgga tcgcctcttt cgggttatcc gggccgacgc tgattaagcg
    29341 cagctgacca attttctcgt caactgtcat tttcttaagc agttcggtga cgaacgcatc
    29401 ccgcgcttcg ggcgttaatg gatggttgcc gaataaatca tccgccagtg ctggctgcag
    29461 ggccagactc accgcgattc ctactgaaca tagccatttc atatggattt ttcttccttt
    29521 gttgcccgac gtggcagcga aaatggtgca aaaaccgtag tttgccataa gcatgatgga
    29581 gagagaaaaa gaatgctcag tttattgtct gaattttcaa aatattcact cgctgaattg
    29641 tcatacaagg cgctattcta gtttgtgata ttttttcgcc accacaagga gtggaaaatg
    29701 tcttccatga caacaactga taataaagcc tttttgaatg aacttgcccg tctggtcggt
    29761 cattcacacc tgctcaccga tcccgccaaa acggcccgct atcgcaaggg cttccgttct
    29821 ggtcagggcg acgcgctggc tgtcgttttc cctggctcac tactggaatt gtggcgggtg
    29881 ctgaaagcct gcgtcaccgc tgacaaaatt attctgatgc aggctgccaa tacaggcctg
    29941 accgaaggat cgacgccaaa cggtaacgat tatgatcgcg atatcgtgat catcagcacc
    30001 ctgcgtctcg acaagctgca cattctcggc aagggcgaac aagtgctggc ctatccgggc
    30061 accacgctct attcactgga aaaagccctc aaaccgctgg gacgcgaacc gcactcagtg
    30121 attggatcat cgtgtatagg cgcatcggtc atcggcggta tttgtaacaa ctcgggcggc
    30181 tcgctggtgc aacgtggccc ggcgtatacc gaaatgtcat tattcgcgcg tataaatgaa
    30241 gatggcaaac tgacgctggt gaaccatctg gggattgatc tgggcgaaac gccggagcag
    30301 atccttagca agctggatga cgatcgcatc aaagatgacg atgtgcgcca cgatggtcgt
    30361 cacgcccacg attatgacta tgtccaccgc gttcgtgata ttgaagccga cacgcccgca
    30421 cgttataacg ctgatccgga tcggttattt gaatcttctg gttgcgcagg gaagctggcc
    30481 gtctttgcag tacgtcttga taccttcgaa gcggaaaaaa atcagcaggt gttttatatc
    30541 ggcaccaacc agccggaagt gctgaccgaa atccgccgtc atattctggc taatttcgaa
    30601 aatctgccgg ttgccgggga atatatgcac cgggatatct acgatattgc ggaaaaatac
    30661 ggcaaagaca ccttcctgat gattgataag ttaggcaccg acaagatgcc gttcttcttt
    30721 aatctcaagg gacgcaccga tgcgatgctg gagaaagtga aattcttccg tccgcatttt
    30781 accgaccgtg caatgcaaaa attcggtcac ctgttcccca gccatttacc gccgcgcatg
    30841 aaaaactggc gcgataaata cgagcatcat ctgctgttaa aaatggcggg cgatggcgtc
    30901 ggtgaagcca aatcgtggct ggtagattat ttcaaacagg ccgagggcga tttctttgtc
    30961 tgtacgccgg aggaaggcag caaagcgttt ttacaccgtt tcgccgctgc gggcgcagca
    31021 attcgttatc aggctgtgca ttccgatgaa gtcgaagaca ttctggcgct ggatatcgct
    31081 ctgcggcgta acgacaccga atggtatgag catttaccgc cggagatcga cagccagctg
    31141 gtgcacaagc tctattatgg ccattttatg tgctatgtct tccatcagga ttacatcgtg
    31201 aaaaaaggcg tggatgtgca tgtgttgaaa gaacagatgc tggaactgct tcagcagcgt
    31261 ggcgcgcaat accctgccga acataacgtc ggtcatttgt ataaagcacc ggagacgttg
    31321 cagaagtttt atcgcgagaa cgatccgacc aacagtatga atccggggat cggtaaaacc
    31381 agtaagcgga aaaactggca ggaagtggag taataataac ggatggcaga gtatcgccat
    31441 ccgtcattca tttaatcgtt ctgtgccgtc tgccccgccg ccgccatttg ggcggctttt
    31501 tgttttttat agctcaacgc tgctgccgga acaggcatca ctttaccggt ttcaatccag
    31561 gtacgcaggc ggctggcatc ggcaaaatgg gtatatttgc caaacgcgtc catcactacc
    31621 agcgccaccg gtttattatt gataaccgtg cgcatcacca gacaatggcc cgccgcattg
    31681 gtaaaaccgg ttttggttaa ctgaatattc cagttatcac gatacaccag atggttagta
    31741 ttgcggaacg gcagcgtata cgtcgggtta gagaaggttg ccatatcttc ccgggtagta
    31801 cttaactgcc cgatcaacgg atattgtttg ctggcaataa gtagtttggt taagtcacgg
    31861 gcggttgaaa cgttatgcac cgacaatccg gttggttcaa caaagcgcgt gttgttcatt
    31921 ccgagcgatt tcgctttcgc attcatcgcc ttaataaagg ctttgtaacc accgggataa
    31981 tggtgcgcaa ggcttgccgc cgcgcggttt tctgaagaca tcagcgccag caacagcata
    32041 tctttacggc tgatttcgct gttcagtcgt acgcgcgaat agaccccttt catctccggc
    32101 gtctggctga tatccacttt tagtttttca tccagcggca gtcgtgcatc cagcacaacc
    32161 atcgcggtca ttaatttgct gatagacgca atcggacgca ccaaatccgg gtggttcgaa
    32221 tagatcactt tgttggtatt cagatcaaca atcatcgcgc taccggaggc aatttccggt
    32281 tgtgacgcgg tggtagcggc tgccgttttc gcaacggcct gcggtgcaaa aggcacagcc
    32341 agcatcaggg ccaggctaaa taaagaaact cgaaatttcg gcatgatgag cattcagata
    32401 gtggttcacg cgcacgggtt gcgcaccgcc ggagtaagga tttactgagg ctagcgacgc
    32461 catcataacg agcaaaaagt gcgatcgtca aagaagaatc gtgaggaaat gctgcattgc
    32521 tgacatttac gccagcaatg cagcgcaaaa gaactttcta gaacaaacga taaccgtagc
    32581 cccatagtat aacggtaagg gcgagcagca cttccagcac cagcacgcct atcgccagtg
    32641 tcgaactgga gaagctaagg ccttcctctt tgttgatatt caggaagctc ggaataccga
    32701 gatacagcag gtagccggtg taaaacagcg ccaccgtgcc gaccagcgca cacaaccaga
    32761 ccagcgggta aagcgccacc agaccgctta aaaacagcgg agttgcaacg tagcccgcga
    32821 agaccatgca gtgcgcaagt gacggacgct gcgggtaatt gcgcgccatc caccagatga
    32881 cccgccccat caccgcgacc ccagcgagca taacgccgta aaacaagaca gccagcgcca
    32941 gtccggtaaa ccaggataac ttcaggatag tgccatcgcc aaaattccag ccaatctgtg
    33001 tagtgccaat gaaggcgcaa atcaccggaa tcgccgccat cagcaaaacg tggtgggtgt
    33061 aatggtgaga aatcgtttcg ttttcacgat taatcacctg catttcacga tcgggatggg
    33121 aaaacagacc ccagacatgg ctcataccgc ccccttgttg tgagttcatg aacctgacag
    33181 ttcaagtata agtcagcttg tgattatttt ttgttcactc atgtaatttg caccgtctga
    33241 tgtacgtcct ttttcagcgt gaatgattca cagggtgtat gcttacaggc aagcaaaggg
    33301 agacagactg tcctatggat ctcaatacac tcatctcaca atatggttat gccgcgctgg
    33361 tgatcggtag cctggcggaa ggtgaaaccg tgactttgct gggaggcgtt gcggcacatc
    33421 aggggctatt aaagttcccg ctggtggtac tttctgtggc gcttggcggc atgattggcg
    33481 accaggtgct ttatctgtgc gggcggcggt ttggcggcaa gctgttacgc cgtttctcga
    33541 aacatcagga taaaattgag cgggcgcaga aacttatcca acgccatccg tatctgtttg
    33601 tcattggtac gcgttttatg tatggcttta gggtgattgg cccgacgctg attggtgcca
    33661 gccagctgcc gccgaaaatc tttctgccgc tgaatattct cggcgcattt gcctgggcgt
    33721 tgatttttac cactatcggt tatgctggtg gtcaggtgat tgcgccgtgg ttgcacaatc
    33781 tcgaccagca tttgaagcac tgggtctggt tgattctggt cgtggttctg gtggtaggcg
    33841 tgcgctggtg gctgaaacga cgcgggaaga aaaagccgga taatcaggcg taaaactatt
    33901 gcctaggata aggcgttcgc accgcatccg acatccggtg caccatgcct gatgcgacgc
    33961 ttgtgcgtct tatcatgcct acccaaatat tacccattga aataaccacg cacctcactg
    34021 cgctattctg ggttgaactg cggattcgcc aacataaagc cgccatccac tatcaacgac
    34081 tgcccggtgg tgtaatttgc gccctccgaa caaagccata ccaccaggct ggcaatctca
    34141 tgcgttgtgc caaaacgtcg caagggaatc gaaggctccg cgtcgggctt cacgtccccg
    34201 tcatccatgc cgttcattgg cgtggcgatc gccccaggtg caaccgcatt caccaaaatc
    34261 ttatgcctga ccagctccag cgccatcgct ttggttaacc cgccgagcgc atgtttagcg
    34321 gctgtgtagg cgctggcatc cggcaacggc gtatgttcat gcaccgacgt aatgttgatg
    34381 atgcgaccgc cctgcccttg tttcaccatc tgacgagccg caatttgcga gcataagaat
    34441 gcaccgtcga catcaacggt aaaaatcttg cgccactcat caaaagccat atcaagaaac
    34501 ggcgctttgg tcattgcacc cgcattattc accagcacat cgatgcgccc cagtcgttga
    34561 atgagtttct ccagcgcctg cgcaccttct ggcagtttac cgagatccag ctgcacgatc
    34621 tccgcacgtg cgccgtggct aaccacctca cgcgcagtat cttttgcccc ttcttcatca
    34681 gagtgccagg taatgccaat atcaaacccc tgctgcgcca gtaataacgc gcactctttg
    34741 ccgatccccg aatcggaggc ggtaataatc gcaacctgtg ccatcgagtt ctccacttaa
    34801 cgctgaataa acgttaagta tagaaggcgc atatcatcag cgtttgtaac ccccgcccaa
    34861 cgcaccagtg agttgaatgg aggcatccag ccactgccct tgcaataaca ggccattggc
    34921 ccgctcgcga agcgccggga ttctggcttc gctgacgcgg gaaccggcaa tgatgcccgc
    34981 gttaaagcgc gcctgtgcaa gacctactac acgcaaggca tcgcgctcaa tttgcgcctg
    35041 atgctggttt ttctccgcca gtgtctgaac ctgactggct gcccgcgcca cgtcattcac
    35101 cgcgtcaacc accgctttgt tgtagctggc gatagacaag ttgctttcgg cttttgcgat
    35161 atcgaggttg gcgttaagac gaccactatc gaaaatgggt agcgtcaggc ctgccgtaac
    35221 gcccatttgc tgcgcggaat gacggaacag atcgcttaag tgcaacgcat cctgttgcag
    35281 gaaggccatc aggttgatgt caggataaaa tgccgctttt gccgcatcaa tagtgcttaa
    35341 cgatgactca acgtaccagt gcgccgcctg caaatctgcc cgccgggcca gtaaggagta
    35401 ccccagttca tcaggaagct ggcttgccac tttcggcaac gcgaccggat taagcttcaa
    35461 tgcctttgtc tggtgatttg taagtgcact taaccgtgcc tcaataattt tcattttccc
    35521 cgcgacatcg ttgagctgct gccgggtttt gctggcatta atatcggttt ccacaccttc
    35581 aactgaagaa gtaatcccgt tctgatatag ctggcgatcg gtggcgataa tggtgttctg
    35641 ctctttttcg atttgctgca agaccgtgtt taacgccgcc tgggtttgcc actcccagta
    35701 caggcgggct acgctgccag ccagcaattg gcgggtttgc tcgcgttccg ccgctcgtgc
    35761 tttaaccgta cccaggcgag cagttacctc cgcccgattc tttccccaga tatcgagatg
    35821 ccagcccgcc gttaagccaa aagtaccgtt ggtgtaccac ggaccggtcg tacctgcggc
    35881 aggatcgttc agagcaaacg gccccattaa cccttctgcc gacatttttt gccgctccat
    35941 atccgcagaa aagtcgatct gcggaccatc ctgagtggca actgccttcg cctgggcttc
    36001 agccagctga atgcgctgtt cagccacctg catatccggt gcgttctgta gtgcgttatt
    36061 aattaaggaa gtgagttgat tatcgtgata ctccagccac cattggctgt ctggccaacc
    36121 atttttcagc gccgtgggta atgcggtgtc aacttgtgaa gcgggcgttt gctggcttaa
    36181 cgcctggcgg gtttcatgca taggcgcaca cccggccagc atcagtaaca gcagaaaaca
    36241 ggcgatggct ggataaaaag aatcacgatt catgggggaa taatcaggta agaaaaggtg
    36301 cgcggagatt accgtgtgtt gcgatatatt ttttagtttc gcgtggcaat acatcagtag
    36361 caataaaacg acatatccag aaaaatatac actaagtgaa tgatatcttc cgatttatct
    36421 taatcgtcta tggataacgg caaagggctt cgttttttcc tatacttatt cagcactcac
    36481 aaataaagga acgccaatga aaattatact ctgggctgta ttgattattt tcctgattgg
    36541 gctactggtg gtgactggcg tatttaagat gatattttaa aaatcaaata tggcatataa
    36601 gttgtcaggt cagtaactag atattttgtg tacttgattt gcaaacacct c
//
LOCUS       DAPHCV010000043        36391 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.043, whole genome shotgun sequence.
ACCESSION   DAPHCV010000043 DAPHCV010000000
VERSION     DAPHCV010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.043"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(261..337)
                     /locus_tag="RH033_002100"
     tRNA            complement(261..337)
                     /locus_tag="RH033_002100"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(301..303),aa:Arg,seq:acg)
     gene            complement(341..433)
                     /locus_tag="RH033_002101"
     tRNA            complement(341..433)
                     /locus_tag="RH033_002101"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(397..399),aa:Ser,seq:gct)
     gene            complement(749..934)
                     /gene="csrA"
                     /locus_tag="RH033_002102"
     CDS             complement(749..934)
                     /gene="csrA"
                     /locus_tag="RH033_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDV2991282.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1169..3799)
                     /gene="alaS"
                     /locus_tag="RH033_002103"
     CDS             complement(1169..3799)
                     /gene="alaS"
                     /locus_tag="RH033_002103"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDV2991283.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFVVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3927..4427)
                     /gene="recX"
                     /locus_tag="RH033_002104"
     CDS             complement(3927..4427)
                     /gene="recX"
                     /locus_tag="RH033_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDV2991284.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4495..5556)
                     /gene="recA"
                     /locus_tag="RH033_002105"
     CDS             complement(4495..5556)
                     /gene="recA"
                     /locus_tag="RH033_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDV2991285.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5636..6133)
                     /gene="pncC"
                     /locus_tag="RH033_002106"
     CDS             complement(5636..6133)
                     /gene="pncC"
                     /locus_tag="RH033_002106"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HDV2991286.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6278..7363)
                     /gene="mltB"
                     /locus_tag="RH033_002107"
     CDS             complement(6278..7363)
                     /gene="mltB"
                     /locus_tag="RH033_002107"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HDV2991287.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7619..8182
                     /gene="srlA"
                     /locus_tag="RH033_002108"
                     /gene_synonym="gutA"
     CDS             7619..8182
                     /gene="srlA"
                     /locus_tag="RH033_002108"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDV2991288.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8179..9138
                     /gene="srlE"
                     /locus_tag="RH033_002109"
                     /gene_synonym="gutE"
     CDS             8179..9138
                     /gene="srlE"
                     /locus_tag="RH033_002109"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDV2991289.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGEATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9149..9520
                     /gene="srlB"
                     /locus_tag="RH033_002110"
                     /gene_synonym="gutB"
     CDS             9149..9520
                     /gene="srlB"
                     /locus_tag="RH033_002110"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDV2991290.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHHYPVTAVGSVAEDNLRELGHVTLRFDGLSEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9524..10303
                     /gene="srlD"
                     /locus_tag="RH033_002111"
     CDS             9524..10303
                     /gene="srlD"
                     /locus_tag="RH033_002111"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDV2991291.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGTAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPEQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10409..10768
                     /gene="gutM"
                     /locus_tag="RH033_002112"
     CDS             10409..10768
                     /gene="gutM"
                     /locus_tag="RH033_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDV2991292.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDYQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10835..11608
                     /gene="srlR"
                     /locus_tag="RH033_002113"
                     /gene_synonym="gutR"
     CDS             10835..11608
                     /gene="srlR"
                     /locus_tag="RH033_002113"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HDV2991293.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVGFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11601..12566
                     /gene="gutQ"
                     /locus_tag="RH033_002114"
     CDS             11601..12566
                     /gene="gutQ"
                     /locus_tag="RH033_002114"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HDV2991294.1"
                     /translation="MSEALLNAGRQTLMLELQEASHLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDDQRLVKGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12563..14077)
                     /gene="norR"
                     /locus_tag="RH033_002115"
     CDS             complement(12563..14077)
                     /gene="norR"
                     /locus_tag="RH033_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HDV2991295.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYNFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14264..15703
                     /gene="norV"
                     /locus_tag="RH033_002116"
     CDS             14264..15703
                     /gene="norV"
                     /locus_tag="RH033_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HDV2991296.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVEEETSAATTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWREVPDNFLCPECSLGKDVFDELASEAK"
     gene            15700..16833
                     /gene="norW"
                     /locus_tag="RH033_002117"
     CDS             15700..16833
                     /gene="norW"
                     /locus_tag="RH033_002117"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HDV2991297.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRNARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILAALMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPV"
     gene            complement(17057..19309)
                     /gene="hypF"
                     /locus_tag="RH033_002118"
     CDS             complement(17057..19309)
                     /gene="hypF"
                     /locus_tag="RH033_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HDV2991298.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRWRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGYHLACDARNSNAVATLRARKHRPAKPLAVMLPVAEGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHHWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECQHLGGLPAVALAGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAGLGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLSWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19462..19989)
                     /gene="hydN"
                     /locus_tag="RH033_002119"
     CDS             complement(19462..19989)
                     /gene="hydN"
                     /locus_tag="RH033_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HDV2991299.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(20138..21148)
                     /gene="ascG"
                     /locus_tag="RH033_002120"
     CDS             complement(20138..21148)
                     /gene="ascG"
                     /locus_tag="RH033_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HDV2991300.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELITAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIAFNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGAYTPPKTFSGKLIRRDSLIAL
                     SR"
     gene            21408..22865
                     /gene="ascF"
                     /locus_tag="RH033_002121"
     CDS             21408..22865
                     /gene="ascF"
                     /locus_tag="RH033_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HDV2991301.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTISGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDSITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEATAEARKHQSAQPTVAKEVSLN"
     gene            22874..24298
                     /gene="ascB"
                     /locus_tag="RH033_002122"
     CDS             22874..24298
                     /gene="ascB"
                     /locus_tag="RH033_002122"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HDV2991302.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAYREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     ELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLIASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LARTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24456..24926)
                     /gene="hycI"
                     /locus_tag="RH033_002123"
     CDS             complement(24456..24926)
                     /gene="hycI"
                     /locus_tag="RH033_002123"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HDV2991303.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGHWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(24919..25329)
                     /gene="hycH"
                     /locus_tag="RH033_002124"
     CDS             complement(24919..25329)
                     /gene="hycH"
                     /locus_tag="RH033_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HDV2991304.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(25326..26093)
                     /gene="hycG"
                     /locus_tag="RH033_002125"
     CDS             complement(25326..26093)
                     /gene="hycG"
                     /locus_tag="RH033_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HDV2991305.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(26093..26635)
                     /gene="hycF"
                     /locus_tag="RH033_002126"
     CDS             complement(26093..26635)
                     /gene="hycF"
                     /locus_tag="RH033_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HDV2991306.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(26645..28354)
                     /gene="hycE"
                     /locus_tag="RH033_002127"
     CDS             complement(26645..28354)
                     /gene="hycE"
                     /locus_tag="RH033_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HDV2991307.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWITVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(28372..29295)
                     /gene="hycD"
                     /locus_tag="RH033_002128"
     CDS             complement(28372..29295)
                     /gene="hycD"
                     /locus_tag="RH033_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HDV2991308.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(29298..31124)
                     /gene="hycC"
                     /locus_tag="RH033_002129"
     CDS             complement(29298..31124)
                     /gene="hycC"
                     /locus_tag="RH033_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HDV2991309.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(31121..31732)
                     /gene="hycB"
                     /locus_tag="RH033_002130"
     CDS             complement(31121..31732)
                     /gene="hycB"
                     /locus_tag="RH033_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HDV2991310.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(31857..32318)
                     /gene="hycA"
                     /locus_tag="RH033_002131"
     CDS             complement(31857..32318)
                     /gene="hycA"
                     /locus_tag="RH033_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HDV2991311.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            32530..32880
                     /gene="hypA"
                     /locus_tag="RH033_002132"
     CDS             32530..32880
                     /gene="hypA"
                     /locus_tag="RH033_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HDV2991312.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            32884..33756
                     /gene="hypB"
                     /locus_tag="RH033_002133"
     CDS             32884..33756
                     /gene="hypB"
                     /locus_tag="RH033_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HDV2991313.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            33747..34019
                     /gene="hypC"
                     /locus_tag="RH033_002134"
     CDS             33747..34019
                     /gene="hypC"
                     /locus_tag="RH033_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HDV2991314.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            34019..35140
                     /gene="hypD"
                     /locus_tag="RH033_002135"
     CDS             34019..35140
                     /gene="hypD"
                     /locus_tag="RH033_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HDV2991315.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAETHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            35137..36147
                     /gene="hypE"
                     /locus_tag="RH033_002136"
     CDS             35137..36147
                     /gene="hypE"
                     /locus_tag="RH033_002136"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HDV2991316.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIELSEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            36221..>36391
                     /locus_tag="RH033_002137"
     CDS             36221..>36391
                     /locus_tag="RH033_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2991317.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQ"
BASE COUNT         8477 a        10142 c         9523 g         8249 t
ORIGIN      
        1 gattcgaacc tccgaccgct cggttcgtag ccgagtactc tatccagctg agctacggat
       61 gcatcgggaa acttacttta ctgcagattt tttgataccg ctactaaagc cgtatcaagt
      121 aagagatggt gcatccggga ggattactcg ccttgcggct cgcccttcgg gccgttgcta
      181 aagcaacgtt atcctccctg gtgcctgcgg ttattaccgc cacctcttcc ataccttcat
      241 cgctgaaggt acgtagaata tggtgcatcc gggaggattc gaacctccga ccgctcggtt
      301 cgtagccgag tactctatcc agctgagcta cggatgcaaa tggcggtgag gcggggattc
      361 gaaccccgga tgcagctttt gaccgcatac tcccttagca ggggagcgcc ttcagcctct
      421 cggccacctc accacacgcc tcttacgagt gcttcgaaga acttgtttat cgctcatcgt
      481 cgctgcgtgg cgcacatatt actttctgag acttataagt caaacaattt ttcccacact
      541 tttatcgttt gcacacttca cgttcaatta gtctgtaaaa acgtcaaaaa gagtgtttta
      601 tcaacagaaa aatggaggtc tgacagatag cagtaatgta aaaaaatgga gacttaagtt
      661 gaatgaacgg gagtaaagcg aaaagactat agagtgaagg agaaattttg agggtgcgtc
      721 tcaccgataa agatgagacg cggaaagatt agtaactgga ctgctgggat ttttcagcct
      781 ggatacgctg gtagatctct tcacggtgaa cagaaacttc cttcggggca tttacgccaa
      841 tacgtacctg gttgcccttt acccctaaaa ctgtcacggt gacctcatcc ccaatcatga
      901 gggtctcacc aactcgacga gtcagaatca gcattctttg ctccttgaaa gattaaaaga
      961 gtcgggtctc tctgtatccc ggcattatcc atcatataac gccaaaaagt aagcaatgac
     1021 aaacacatta catctaagca gtcatggcat tacattctgt taaacctaag tttagccgat
     1081 atacacaact tcaacctgac tttatcgttg tcgatagcgt taatgcgaat gccgtgaagc
     1141 gagtccacgg cattgcctta cgcttatatt attgcaattt cgcgctgacc cagcctttca
     1201 cactggctaa cgctgcaggt aaggccgcag catccgtacc accggcttgc gccatgtcag
     1261 gacgtccacc acccttgccg cccacctgct gagcgaccat accaatcagt tcccctgctt
     1321 tcacacgatc tgtgacgtcc ttagatacgc ctgcaatcag agaaacctta ccttcggcta
     1381 ccgttgccag cacgataatt gtcgacccca gctgattttt taaatcgtca accatggtac
     1441 gcaacatttt cggctcaaca ccgctaagct cgctaaccaa cagcttaaca ccattaacat
     1501 caattgcctt actggaaaga tttgcgctct cctgtgcggc agcttgttct ttaagctgtt
     1561 gtagttcttt ttctaactga cgcgtacgtt ccagtactga gcgcacttta tcagccagat
     1621 tattgctatc gcctttcagc agatgtgcga cttcgcttaa gcgatcgctg tctgcatgaa
     1681 cggtggcgat agcgccttct ccggttaccg cttcaatacg acgaacgcct gcagcagtac
     1741 ccgattcaga gatgatgcgg aacagaccaa tatcaccagt gcggctggcg tgagtaccgc
     1801 cacacagctc ggtggagaaa tcgcccatgc tcagcacgcg tacgcgctca tcatacttct
     1861 cgccgaacag cgccatcgca cctttcgctt tcgccgcttc gagatccatg atgttggttt
     1921 cgatcggcaa attgcgacga atctgtgcgt tcaccaggtc ttcgaccgca cgaatctctt
     1981 ccggtttcat cgcttcgttg tgtgagaagt cgaagcgcag caccttgtcg ttaaccagtg
     2041 aacctttctg cgatacatga gtacccagaa cctggcgcag cgcagcgtgc atcaggtgcg
     2101 ttgcggagtg attcagacga atacgggcgc gacgagcctc atcaacatca gcctgcacag
     2161 cgtcgcccac tttcagagaa cccgcagcaa gtttaccgat gtgaccaatc gcctggccgt
     2221 atttctgcgt atcttccacc acgaaggaga agttagcgcc tttcagttcg cctttatcac
     2281 caacctgacc gccggattcc gcatagaatg gcgtttgatc cagcacgacc acagcttcct
     2341 ggcctgcatt gatggcatca accgctttac catcaacaaa cagcgcggtc actttgccgt
     2401 tcagttccag atggtcatag cctttaaatt cagatgcact gtcaacacgg atcattgcgt
     2461 tgtaatcggc accaaagccg ctggcttcgc gcgcgcgacg acgctgctct tccattgctg
     2521 cttcaaaacc agcttcgtca actttgatgt tgcgctcacg acaaacgtca gccgtcaggt
     2581 caaccgggaa gccataggtg tcgtacagac ggaaagcagt ttcaccatcc agcgtatcac
     2641 cagaaagttt tgccagctct tcatccagca acgccagacc gcgctccaga gtacgagcaa
     2701 actgctcttc ttcagtcttc agcacctgct caacctgcgc ctgctggcgt ttcaggtctt
     2761 cacccgcaga gcccataacg tcgatcagcg gaccaaccag tttgtagaag aaggtttctt
     2821 tcgcgccgag catattaccg tgacgcactg cgcgacgaat gatacgacgc agtacataac
     2881 cacggttttc attggacggc attacgccat ccgcgatcag gaacgcacaa gaacgaatgt
     2941 ggtcagcgat tacgcgcagc gatttattgc tcagatcggt tgcgccagtg actttcgcta
     3001 ccgcctggat caacgtgcgg aacaggtcga tgtcatagtt agagttaacg tgttgcagca
     3061 ccgcagcaat acgctccaga cccataccgg tatctacaga cggcttcggc agcggttcca
     3121 tcgtgccatc ggcctggcgg ttgaactgca tgaagacgat gttccagatc tcaatgtagc
     3181 ggtcgccgtc ttcttccgga cttcccggag ggccacccca aatgtggtcg ccgtgatcgt
     3241 agaagatttc ggtgcacggg ccgcacggac cagtgtcacc catctgccag aagttgtcag
     3301 atgcgtatgg cgcacctttg ttatcgccga tgcgaataat acgttcgcgc gggatcccta
     3361 cttctttttc ccagatttcg taggcttcgt cgtcgctttc atagacggta acccacagac
     3421 gctctttcgg cagggcaaac catttttcgc tggtcagcag ttcccatgca aactgaatgg
     3481 catcgtgttt gaaatagtcg ccgaagctga agttgcccag catttcgaag aaggtatggt
     3541 gacgcgcggt gtaaccgacg ttttccaggt cgttgtgttt accacccgca cgcacgcagc
     3601 gttgggaagt ggtagcgcgg gaataattac gcttgtcgag cccaaggaac acatccttga
     3661 actggttcat cccggcgttg gtaaacaaca aagttgggtc gttatggggt accagggagc
     3721 tgctggcaac tacctgatgt cccttactat ggaaaaagtc gagaaacgcc tgacggatct
     3781 cagcggtgct cttgctcata attatcctga aatcaagcta acgaaatatc gccaccagct
     3841 ccagcgtgtc ttaaccgccg ggctggtaac tgaaaagtgg gaataagata agttttcttg
     3901 actgggaagt aaaataccgt atgcgttcag tcggcaaaat ttcgccaaat atcctggata
     3961 tcttccatca gatagccacg atagagcaga aaacgctgga tcttaacttt ttctgaaaag
     4021 acagttggca aaggttcacc atattttcgc gtcgcctgat cgcgcgccag tgcgcaccag
     4081 tcgatgtcac attcacgcat cgctttttct gtcgcttcgc gggaaatacc tttctgattc
     4141 agttcctggc gaatacgcgc aggtccataa cctttgcggc tacggctggc gataaagcgc
     4201 gcaacaaatc ggctgtcatc gagatagcca tgttcatggc accaggcaat aacgcgctcg
     4261 taatcttctg ccgtagcatc aatctcttct gggccatttt tgcccataat cggtgccgcg
     4321 agtttacgtc gcagttcttg ctcactgtga tcgcgcaccg ccagaatgcg taccgcacga
     4381 tccaacaggc gagcatatgc cgggcgacgg gacgttgatt ctgtcatggc atatccttac
     4441 aacttaaaaa gcaaaagggc cgcagatgcg acccttgtgt atcaaacaag acgattaaaa
     4501 atcttcgtta gtttctgcta cgccttcgct atcatctaca gagaaatccg gcgttgagtt
     4561 cgggttgctc agcagcaact cacgtacttt cttctcgatc tctttcgcgg tttccgggtt
     4621 atctttcagc caggcagttg cattcgcttt accctgaccg atcttctcac ctttgtagct
     4681 gtaccacgcg cctgctttct cgatcagctt ctcttttacg cccaggtcaa ccagttcgcc
     4741 gtagaagttg ataccttcgc cgtagaggat ctggaattca gcctgtttaa acggcgcagc
     4801 gattttgttc ttcaccactt tcacgcgggt ttcgctaccc accacgtttt cgccctcttt
     4861 caccgcgccg atacgacgga tgtcgagacg aacagaggcg tagaatttca gcgcgttacc
     4921 accggtagtg gtttccgggt taccgaacat cacaccaatt ttcatacgga tctggttgat
     4981 gaagatcagc agcgtgttgg actgcttcag gttacccgcc agcttacgca tcgcctggct
     5041 catcatacgt gccgcaaggc ccatgtgaga gtcgccgatt tcgccttcga tttccgcttt
     5101 cggcgtcagt gccgccacgg agtcaacgac gataacgtct actgcgccag aacgcgccag
     5161 ggcgtcacag atttccagtg cctgctcgcc ggtgtccggc tgggagcaca gcaggttgtc
     5221 gatatcgacg cccagtttac gtgcgtagat tgggtccagc gcgtgttcag catcgataaa
     5281 cgcacaggtt ttaccttcac gctgcgctgc ggcgatcacc tgcaacgtca gcgtggtttt
     5341 accggaagat tccggtccgt agatttcgac gatacggccc atcggcagac cacctgcccc
     5401 aagcgcgata tccagtgaaa gcgaaccggt agagatggtt tccacatcca tggaacggtc
     5461 ttcacccagg cgcatgatgg agcctttacc aaattgtttc tcaatctggc ccagtgctgc
     5521 cgccaacgct ttctgtttgt tttcgtcgat agccattttt actcctgtca tgccgggtaa
     5581 taccggatag tcaatatgtt ctgttgaagc aattatactg tatgctcata cagtatcaag
     5641 tgttttgtag aaattgttgc cacaaggtct gcaatgcata cgcagtagcc tgacggcgca
     5701 ccgcatcacg gtcgccgctg aagcattccc gccgggtaat gccttcaccg cgggcagtgg
     5761 caaaagcaaa ccagacggtg ccgacaggct tctcttcact gccgccatcc ggcccggcga
     5821 taccactaat agacacggcg taatcagcac gagccgcttt cagtgcgcct atcgccattt
     5881 ccaccacgac gggttcgctc accgcgccat gctgcgccag cgtctcttcg cgtacgccga
     5941 tcatttgcgc tttcgcttcg ttactgtagg tgacaaatcc gcgttcaaac caggcggaac
     6001 tgccggcaat atcggtaatc actttcgcta cccaaccgcc ggtacaagac tcagcagttg
     6061 ttacggttgc gccacgggct ttcagcgcct gcccaacctg ttcgcttaac tgcatcagtt
     6121 cactgtcagt catcacaacc tctgtcagtc aaaaatttat gccggacaag atagcacttt
     6181 cgtcaggaag atggtgttgg cgttgcaatt ttacgaggag gattcagaaa aaagctgatt
     6241 agccagaggg aagctcacgc ccccctcttg caaatagtta ctgcacgcgc gccagcgcca
     6301 cggcttgtcc taactgccag accgccattg cgtaatgggt gctgtggtta taacgggtaa
     6361 tggtgtagaa gttcggcaga ccgtaccagt actggtaacc ggtaccgaca tccagacgca
     6421 gcaggctggc ttgttgatgg ttgcccagcg gctgctgtgg cgttaaacct gccgcggcaa
     6481 gctgcgaaat gctgtacctg gttttgaagc catttggcaa gcctggagcc tgaccgtttg
     6541 ccattaccgc gacctgatcg cctttcaccc agccgtgcgc tttgaaatag ttcgccacgc
     6601 taccgatcgc atcaaccgga tcccacaggt tgatatgccc atcgccgctg aaatccactg
     6661 catattgttt gtaagacgac ggcataaact gtccgtagcc catcgccccg gcaaaggaac
     6721 ctttcagatt gagcggatcg tcctgctcgt cgcgcgccat caacaggaag gtttccagct
     6781 cgccagagaa atactccgcg cggcgtgggt agttaaatga cagcgttgcc agcgcatcga
     6841 ggatgcgagt tttccccatc acgcgccccc agcgggtttc aacgccgata atcccgacga
     6901 taatttccgg cggtacgcca tacacctgcc acgcgcgatt caacgcatct tcatactgat
     6961 tccagaaaac cacaccgttc tgtacattgt ccggcgtaat aaattttttg cgataacgca
     7021 gccatgcgcc gttcggacct gatggcggtt tcaccgatgt cgttggtgcc tggttatcca
     7081 tcagccgcag taccgaatcc agacgtttcg cctgggagag aatttcctgc aactgctgac
     7141 gatcgaaacc gtgtttgttc accattttgt cgatgaactg ctgggcattc gggttattag
     7201 cgaaatcgcc gcccatctgc atcacattgt gctgcggctc aagcaggaag ccgccagacg
     7261 gcgttccggt ggtcgtttca gtctcagtag gttttggctt gctgctacag gcggcaagca
     7321 acacaaaaag gggaagcaat gttacataac gacgcttgaa catgaggggt ccatttaaca
     7381 gattcaacca agggcaagta tggtaaagca tcacgccccg cacaaggaag cggtagtcac
     7441 tgcccgatac ggactttaca taactcaact cattctcctc gctatccttt tactcaaact
     7501 ttcaaattaa aatatttatc tttcattttg cgatcaaaat aacactttta aatctttcaa
     7561 tctgattaga ttaggttgcc gtttggtaat aaaacaataa atcctgaagg agagaacaat
     7621 gatagaaacc attactcatg gtgcagagtg gtttatcggg ctgttccaaa agggcggaga
     7681 ggtgtttacc gggatggtga ccggcattct tccgctgttg attagcctgc tggttatcat
     7741 gaacgcactg attaatttta tcggtcagca tcgtattgaa cgttttgctc aacgttgcgc
     7801 cggtaatcct gtttcccgtt acctgctgtt accgtgcatt ggcacgtttg tcttttgcaa
     7861 tccgatgacc ttaagcctgg gtcgctttat gccggaaaaa tacaaaccca gctactacgc
     7921 agcggcctct tatagctgcc actcaatgaa tggcctcttc cctcatatca accctggcga
     7981 actgtttgtt tatcttggca ttgccagcgg tctgacgacg ctgaatctgc cacttggccc
     8041 actggcagtg agttatctgc tggttggttt ggtcaccaac ttcttccgcg gctgggtaac
     8101 cgatctgacc accgctattt tcgagaaaaa gatgggcatt caacttgaac aaaaagttca
     8161 cctggcagga gcaacatcat gacacgcatt cggatcgaaa aaggaacggg tggctggggc
     8221 ggcccgcttg agctggaagc cacgccgggc aaaaaaatcg tctatatcac cgccggtacc
     8281 cggcctgcga ttgttgacaa actggcacag cttactggct ggcaagctat cgacggattt
     8341 aaagaaggtg aacccgcgga ggcggaaatt ggtgtcgcgg taatcgactg tggcggcaca
     8401 ttacgctgcg gcatctatcc gaaacggcgt attcccacca ttaatatcca ctcgacgggc
     8461 aagtccggcc cgctggcgca gtacattgtg gaagatattt atgtctctgg tgtaaaagaa
     8521 gaaaacatca ctgtagtggg tgaggcgaca ccacaaccct cttccgtggg ccgtgactat
     8581 gacaccagca agaaaatcac cgaacaaagc gatggtttac tggcgaaggt gggaatgggt
     8641 atgggttctg ccgttgccgt gttgtttcaa tctggtcgtg acaccatcga cactgtatta
     8701 aaaaccattc tgccgtttat ggcgttcgtc tcggcgctta tcggcatcat tatggcttcc
     8761 ggtcttggcg actggattgc ccacggtctt gctccgctgg cgagccatcc tctggggctg
     8821 gtcatgctgg cgctcatctg ctccttcccg ctgctttcac ctttcctcgg cccaggcgca
     8881 gttatcgcac aggttatcgg cgtattgatt ggcgtgcaga ttggtctcgg caatattccg
     8941 ccgcatctgg ctttacctgc actgtttgcc atcaacgcgc aggcggcctg cgacttcatc
     9001 ccggtcggtt tgtcgctggc ggaagctcgt caggacacgg ttcgcgtcgg tgtcccttct
     9061 gttctggtga gccgcttttt aaccggcgcg ccaactgtac tgatcgcctg gtttgtctcc
     9121 ggttttatct atcaatagag gctgaaacat gaccgttatt tatcagacca ccatcacccg
     9181 tatcggcgcg agtgccactg acgccctcag cgaccagatg ctcatcacct ttcgtgaagg
     9241 cgcgcctgcg gatctcgaag agtattgctt cattcattgc cacggcgagt tgaaaggtgc
     9301 actccatccc ggtttgcaat tttctctcgg gcagcatcac tatccggtga ccgccgttgg
     9361 cagcgtggcg gaagacaacc ttcgcgaact gggtcatgtc accctgcgct tcgatggttt
     9421 aagcgaagcg gaatttccgg gcactgtcca tgtggcaggc cctgtacccg acgatatcgc
     9481 gccgggatcg gttttgaagt ttgaatctgt taaggagtaa aaaatgaatc aggttgccgt
     9541 tgtcatcggt ggtgggcaaa ccttaggcgc gttcctgtgc cacggtctgg ctgccgaggg
     9601 gtatcgcgtg gcggttgtcg atattcagag cgacaaagcc gcaaatgtgg cacaagaaat
     9661 taacgccgaa tatggtgaag gaacagcgta cggttttggt gctgacgcca ccagcgagca
     9721 aagcgttctg gcgctttctc gtggggtaga tgaaatcttt ggtcgcgtgg atttgctggt
     9781 ctacagcgcc ggaatagcaa aagcagcctt tatcagcgac ttccagctcg gcgattttga
     9841 ccgttcgcta caggtgaatc tggtgggtta tttcctgtgt gcccgtgaat tttcgcgttt
     9901 gatgatccgc gacgggattc agggtcgcat tattcagatc aactcgaaat ccggcaaagt
     9961 gggcagcaaa cacaactctg gctacagcgc agcgaaattt ggtggcgtcg ggctgactca
    10021 atcactggca ctggatctgg cggagtacgg cattacggtg cattcgctga tgctcggtaa
    10081 cctgctgaaa tcgcctatgt tccagtcact gttgccacaa tacgcgacca agctgggtat
    10141 caaaccggag caagtcgagc agtattacat cgataaagta ccgctcaaac gcggctgcga
    10201 ttatcaggat gtgctgaata tgctgctgtt ctacgccagt cctaaggcgt cgtactgcac
    10261 tggtcagtcg atcaatgtca ccggcggtca ggtgatgttc tgattaacag cggagatccg
    10321 ttaaggatct ccgcgcgact aatagaatgc ctgatgcgct acgcttatca ggcctacagg
    10381 acttccgcca ctacattacg gagaagttat ggtatccgca ctcatcaccg tcgccgttat
    10441 cgcctggtgt gcgcaactgg ccttaggcgg ttggcaaatt tctcgtttta accgtgcctt
    10501 cgacacgcta tgccagcaag ggagggttgg cgtgggccgt tccagcgggc gctttaaacc
    10561 gcgggtcgtg gtcgctattg cgctggacta tcagcagcgc atcgtcgaca ccttgtttat
    10621 gaaaggactg accgtcttcg cccgaccgca aaaaattccc gcaattaccg gcatgcatgc
    10681 gggtgattta cagcccgatg tgatctttcc ccatgatcca ctatcacaga atgctctatc
    10741 attggcgctt aaactgaaac gtggataatt tcgttgtgaa tgttacttgc ttgcgaagtt
    10801 atcattttga aacctaaatc aggtaatcac gcccatgaaa cctcgtcagc gtcaggccgc
    10861 cattctggag tatctacaaa agcagggtaa atgctcggtt gaagaattgg cgcaatactt
    10921 tgacaccaca ggcacaacca ttcgcaaaga tctggtcatt ctggaacatg ccggaaccgt
    10981 cattcgtact tatggcggcg tggtgttgaa caaagaggaa tccgatccgc ctatcgatca
    11041 taaaacgctt atcaacaccc acaaaaaaga gctgattgca gaagctgccg ttggttttat
    11101 tcatgatggc gactcaataa ttcttgatgc tggcagtacc gtgttgcaga tggtgcctct
    11161 gctatcgcgc tttaataaca tcacggtgat gaccaacagc ctgcacatcg tcaatgcgct
    11221 atccgaactg gataacgaac aaactatcct gatgccaggc ggaacatttc gcaaaaaatc
    11281 ggcctcattt cacgggcaac tggcagagaa tgccttcgag catttcacct tcgataaatt
    11341 gtttatgggc accgacggca tcgatctcaa tgcgggcgta accaccttta acgaggtcta
    11401 taccgtcagt aaggcgatgt gtaatgccgc gcgcgaagtg attttgatgg ctgactcatc
    11461 gaagtttggt cgtaaaagcc ccaacgtggt ttgtagtctt gaaagcgtcg ataagctgat
    11521 taccgacgca ggtatcgatc cagcgtttcg tcaggcgctg gaagaaaaag ggatcgacgt
    11581 gatcataacc ggagagagca atgagtgaag cactactgaa cgcgggacgt cagacgttaa
    11641 tgctggagtt gcaggaagca agccatttac cggaacgtct gggcgatgat ttcgttcgcg
    11701 ccgccaatat catcctgcac tgcgaaggca aagtggtggt ttcgggaatt ggcaaatcgg
    11761 gccacattgg taagaaaatc gccgcaacgc ttgccagcac cggcacaccg gctttttttg
    11821 tccatccggc agaagcactg cacggcgatc tggggatgat tgaaagccgc gatgtgatgc
    11881 tgtttatctc ttattccggt ggcgcgaagg aactggatct cattattccg cgtctggaag
    11941 acaaatctat cgcgctgctg gcaatgaccg gaaaaccgac gtcaccgctg ggcctggcgg
    12001 cgaaagcggt gctggatatc tccgtagaac gcgaagcctg cccgatgcac cttgcgccga
    12061 cctccagcac cgtgaatacc ctgatgatgg gcgacgcgct ggcgatggca gtcatgcagg
    12121 cgcgtggttt taatgaagaa gattttgccc gctcccaccc agccggggca ctgggcgctc
    12181 gcttgctgaa caaagtgcat catcttatgc gtcgtgacga tgccatcccg caggtggcgt
    12241 taaccgccag cgtgatggac gcgatgctgg aacttagccg caccggttta ggtctggtgg
    12301 cagtgtgcga tgatcaacga ctggtgaaag gcgtctttac cgacggtgat ttacgtcgct
    12361 ggctggttgg cggcggcgca ctcaccacgc cagtcaatga agcgatgacg gtcggcggca
    12421 ccacgttgca atcgcaaagc cgtgccatcg acgccaaaga aatcctgatg aaacgcaaaa
    12481 tcaccgccgc accggtagtg gatgaaaacg gcaaactcac cggcgcaatt aacctgcagg
    12541 atttctatca ggccgggatt atttaatcct tcagccccag acgtttcgcc agccgatgca
    12601 ggttggcgac gtcggtttcc agcatccgcg cacaggcggc ccagttgtga tgattttgcg
    12661 ccagtgcctg acgaatggtt tcacgctgga acgcttctgt cgcttcacgc aggttttgct
    12721 taacaacggg caccgccgcc gcttctggcg gcggcaacgt cacctcagga aaagcaaaat
    12781 gttgcgcctc aagaatcact tcatcgccgc tgcgggttgc tctcgccagc actaccgccc
    12841 gatgaatagc atgttccagt tcacgaacgt tccccggaaa attgtagtgt tgcagtaaat
    12901 ttcgcgctcc ggcacttaat accactcggg agagccccag ccgcaaacga cactgctcgc
    12961 agaaataccc cgccagcaga atgacatcat cgccccgctc acgcagcggc ggcactgaaa
    13021 gtggaaacac gctcaggcgg tggaacaagt cagcgcgaaa tcgccctgcc agtacctctt
    13081 cgcgtaagtc acggttagtc gccgccagca cgcgcacatc gacccgcaaa ctgcggtcat
    13141 cgccaacgcg ttgaatatcg ccatactgca acaccctcag cagcttggcc tgcaatgcca
    13201 acgacaactc gccgatctca tcgagaaaca gcgtgccgtt atccgccatt tcaaacttcc
    13261 cgctgcggtt actgatagca ccagtaaacg cccctttcac atgcccgaac aactcacttt
    13321 ccgccacact ttccggcagt gcggcacagt tgagatagac cagcggattc accgcccgtg
    13381 gcgaggcttc atgaatcgct ttcgccacca gctccttacc ggttcccgtc tcaccgctaa
    13441 ttaaaacgtt gagatcggat gccgccacaa tctcaatctc ttttttcaat tgcgtcatgc
    13501 caggggacaa accaatcatc tgcgtctgtt tcaccgcttc aaacggcgcg gcatcgcctg
    13561 gcagcatatt ctggctttcc agttgttcaa tcagcaatgc attgcttaac gctcccgccg
    13621 ccagtgcggc aatcagccgt agctcttcgt cgctgaaaac atcgaactga tcgggctgca
    13681 tcccgtcgag cgtcaaggca ccgatcaggt tttgcccggc aaacaacggc aaaccaacgc
    13741 aggcgtgaac cttcagactc tcctgccctg gaatcaaacc gtcataagga tcgggcaatt
    13801 cgctgtctgc gggaaagcgc accacatcac ccgcgcgggc aatcgcttcc agccgtggat
    13861 gaccttccag cgcaaagcgt ctaccgagta catccttcgc cagcccgtcg atggcaagcg
    13921 gaataaactg ccgcgagtcg taacgtagca acgcagacgc atcgcactcc agcacctgac
    13981 gcagcgtggt gatcaggcgc tgaaaacgat cctgatgacc aatcccacgc tgcaattcga
    14041 tggcgatatt cgccagcaca tcaacggaaa aactcatctt tgcctcactg tcaatttgac
    14101 tatagatatt gtcatatcga ccattttatt gatagtcatt ttgactactc attaatgagc
    14161 acaattttat ttatagagta aaaacaatta gataaaaaac tggcacgcaa tctgcaatta
    14221 gcaagacatc tttttagaac acgctgaata aattgaggtt gctatgtcta ttgtggtgaa
    14281 aaataacatt cattgggttg gtcaacgtga ctgggaagtg cgtgattttc acggtacaga
    14341 atataaaacg ctgcgcggca gcagctacaa cagctacctc atccgcgaag aaaaaaacgt
    14401 gctgatcgac accgtcgacc ataaattcag ccgcgaattt gtgcagaacc tgcgtaatga
    14461 aatcgatctg gcggatatcg attacatcgt aattaaccat gctgaagagg accacgccgg
    14521 ggcgctgacc gaactgatgg cacaaattcc cgatacgccg atctactgta ctgccaacgc
    14581 tatcgactcg ataaatggtc atcaccatca tccggagtgg aattttaatg tagtgaaaac
    14641 tggcgacacg ctggatatcg gcaacggcaa acaactcatt tttgtcgaaa cgccaatgct
    14701 gcactggccg gacagcatga tgacttacct gacaggcgac gcggtgctgt tcagtaacga
    14761 tgctttcggt cagcactact gcgacgagca tctgttcaac gatgaagtgg atcagacgga
    14821 gcttttcgag cagtgccagc gttactacgc caatatcctg acgccgttca gccgcctggt
    14881 aacgccgaaa attaccgaga tcctgggctt taacttacca gtcgatatga tagcgacctc
    14941 tcacggcgtg gtatggcgcg ataacccgac gcaaattgtc gagctgtacc tgaaatgggc
    15001 ggcggattat caggaagaca gaatcaccat tttctacgac accatgtcga ataatacccg
    15061 catgatggcc gatgctatag cccagggtat tgcggaaacc gacccacgcg tagcggtgaa
    15121 aattttcaac gtcgcccgaa gcgataaaaa cgaaatcctg accaatgtct tccgctcaaa
    15181 aggcgtgctg gtcggcactt cgacgatgaa taacgtgatg atgccgaaaa tcgccgggct
    15241 ggtggaggag atgaccggat tacgcttccg taacaaacgc gccagcgctt tcggctctca
    15301 cggctggagc ggcggtgcgg tggatcgtct ttccacgcgc ctgcaggatg cgggtttcga
    15361 aatgtcgctt agcctgaaag ccaaatggcg accggaccag gacgctctgg agttatgtcg
    15421 tgaacacggt cgcgaaatcg cccgtcagtg ggcgctcgcg ccgctgccac agagcacggt
    15481 gaatacggtg gttgaagaag aaacctctgc cgccacgacg gctgacctcg gcccacggat
    15541 gcaatgcagc gtctgccagt ggatttacga tccggcaaaa ggcgagccaa tgcaggacgt
    15601 tgcgccagga acgccgtggc gtgaagtccc ggataacttc ctgtgcccgg aatgctctct
    15661 cggcaaagac gtctttgacg aactggcatc ggaggcaaaa tgagcaacgg cattgtgatc
    15721 attggttcgg gcttcgccgc ccgccaactg gtgaaaaata ttcgcaaaca ggacgccagt
    15781 attccattaa ccctgattgc cgccgacagc atggatgagt acaacaaacc tgacctcagc
    15841 catgttatca gtcaggggca acgtgccgac gaccttaccc gccagacggc aggtgaattt
    15901 gccgagcagt ttaatctgcg cctgtttccg cacacctggg taacggatat cgatgccgaa
    15961 gcccatgtgg tgaaaagtca gaataatcag tggcaatacg acaagctggt gctggcaacc
    16021 ggtgccagcg cctttgtccc gccagtgccc gggcgtgagt taatgctgac gttaaatagt
    16081 cagcaagagt atcgcgcctg tgaaacgcaa ctgcggaatg cccgacgcgt gttgattgtt
    16141 ggcggtggct tgattggtag cgaactggcg atggattttt gtcgtgcagg caaagcggtc
    16201 acgctaatcg acaacgctgc cagtattctg gcagcgttaa tgccaccgga agtaagcagc
    16261 cgcttgcagc atcggttgac ggagatgggc gttcatctgc tgttaaaatc tcagttgcag
    16321 gggctggaaa aaacggattc tggcattctg gcaacgctgg accgccagcg cagcatcgaa
    16381 gtggatgcgg taattgccgc caccggactg cgcccggaaa ccgccctggc acgacgcgcc
    16441 gggctgacga ttaatcgcgg cgtttgcgtc gatagttatc tgcaaaccag taatgccgat
    16501 atttatgcgc tgggcgattg cgcggaaatt aacggtcagg tattgccgtt cctccagccg
    16561 attcaactta gcgcgatggt gctggcaaaa aatcttctcg gcaataacac gccgctgaaa
    16621 ctcccggcga tgctggtgaa aatcaaaacg ccggaattac cgctgcatct ggcaggcgaa
    16681 acccagcgtc aggatttacg ctggcaaatt aataccgaac gccagggaat ggtggcgcgc
    16741 ggtgttgatg atgccgacca gcttcgcgcc tttgtggtca gtgaggatcg gatgaaagag
    16801 gcgtttggat tgttgaaaac gttgccggtg taggcgagct acagtgcccc aaatgtcgga
    16861 tgcggcgctg acgcgtctta tccgacctac agggaacgca tgtgttgatc acataatacg
    16921 tttatgtcgc atcctgcact gtcccaaaat gtcggatgcg acgctggcgc gtcttatccg
    16981 acctacgggg acgcacgtgt aggccggata aggcgtttac gccgcatccg gcaatggtgt
    17041 ccaaatgcaa cacgctttat ccgttctgga cttcacccgc taaccagcgc gccgcagcaa
    17101 taaccccctg ccccagagac aatccaccat cgcccgccgg taaactctgc gggaagagca
    17161 acgtaaaatc agcaagataa tgcgccagac gtgcacgcag caaacggtta tgaataactc
    17221 cgccgctaaa taccagcgta gtgatacccc gcatcgtggc ctgctcacgc atcaacgcgg
    17281 caaaaccctg cgccagcgca tcatgaaacg cccacgcgcg ttgattaacc ggagcctgcc
    17341 agctcagcca ctgctgccag aaagtggcga gatccagttg attgtccacc agcggcatcg
    17401 tcaccggatg cgtcacgccg tggcacgaag ccgccagcgc ctccagcgca caagccgctt
    17461 caccttcata acttaacgtg gctggcgcac agcccagtcc cgccgccaca gcatcgaaca
    17521 aacgcccaca cgatgacgcc agcggcgcgt taattccacg ctcaatggcc cgcgccagca
    17581 cgctccagtt ttgctgttgc acacttgccg tttcagggta attctgccac tccggcacaa
    17641 agcgcaggca ctgcgccagc aggttacgcc acggctgctt cgccgccaaa tcgccaccag
    17701 caagcgccac cgctggcaag ccgcccaggt gctggcattc gcgatagttc acccgcaggc
    17761 actcgccgcc ccataaagcg ccgttctccc ccataccgat accgtcgagc gtcaacgcaa
    17821 tgacatcacc gccatccagc ggccagtgat gttctgccag acacgccgcc gcatgagcat
    17881 gatgatgcag caccgtttgc gtcggcagat tcatttcgcg cgcccactgg ctggagacat
    17941 agcccggatg cgcgtcatgc acaacgtatt gcggagtgaa atcgtagatg ttttgcatca
    18001 ggcgtaacgc ttcgcgccac tgcatctgga tgccatcgtc acttaaatcg cccagatgct
    18061 ggctcaacac cgcttgttca ccgcgcacca ggcagaaggt atttttcaga tccgcgccga
    18121 gacacagcac aggcggaaca tttttaaagc ccggaggcaa agccagcgca tccggcacat
    18181 acccccgcga acggcgcagc atttcgccgc tttcgcgcac caccgaatca tccatccgct
    18241 gcacgatgtc gcggttatgt atcaagaatc cgtcggcaat gccctgcaaa tccgccagcg
    18301 cctgttcgtt gctgatagcc ggtggtttac cgctcaggtt gccggaggtc atcaccagcg
    18361 ggcattgcag ttcctgtaac agcaaatgct ggagcgggtt cgcaggcaac atcaccccaa
    18421 cttcgttaag gccaggggcg atatcatcac aaagctcagg aacgtatttt ttatccacca
    18481 gcacaatcgg cgcggcgggt gtggtaagca actgacgggc agcgtctggt aaaccttctg
    18541 ccactggcaa cataaccgcc agcggtttcg ccgggcgatg tttgcgcgcc cgcagtgtcg
    18601 ccaccgcgtt actgttacgt gcatcgcagg caagatgata tccgccaatc cctttgatgg
    18661 cgacaatgtt gcccattttt aactgtgcga tagctgcctg taatgccgcc tcttgttctg
    18721 catgttcacc atgacttacc cattcaagat gtgggccaca ctccgggcag gccaccggct
    18781 gggcgtggaa gcgacgatcg agcgggtcgc gatattcttt gtcacaggcc ggacacagcg
    18841 gaaacgccgc catcacggta aacgggcggt cgtaaggcat cgcgcgaata atggtgaaac
    18901 gcggaccaca gtgagtacag ttgataaacg gataacgcca gcgtcgttcg cctggggtat
    18961 tcatttcggc aagacaggca gggcaagtcg cggcatcggg aacaatttgc gtattcatgg
    19021 tgccgcctgt gctctggcgt atagtgaact cggtgggcag ttgtgaccag ataaacggct
    19081 cacgctcgac gctatcaata cgcgccagcg gcgggcagtg ctgatgcaac tgcacaagaa
    19141 acgtttccgg gtcttccagc agccggactt ccactccatc gccgtcatta cagacatcgc
    19201 cgtgaagatt taattgctgt gctagctgcc agacaaacgg acgaaaaccg acgccctgca
    19261 ctttgccacg aatacgcagt tggacaccgc aagatgtgtt ttttgccatt gagttattcc
    19321 cgccatcatg aattgcgtaa cccgccctgc cggacacgac agcgtcgtat ccggcaatca
    19381 caggtcggcg ataccgcccg ctccgtattc tacgaatatt tccgggaatt cctttgatgc
    19441 cagaacagtt cagtaagatt tttagaacat cagcgccgta cggcgacgtt tttctgcact
    19501 cagttgttca agtttattac gatcgacaca aatcagcgca tgggtcgggc aagccgccat
    19561 acacgccggg ccgtcttcac gatggttaca gaggtcgcat ttattggctt cggctttgtc
    19621 agcccgtaca ttcagccccg cgccgctgtt gcgaatcacc ggacgtacca ccacttccat
    19681 cgcgccatac gggcaagcca caacgcaggt tttgcaacca atacaacgtt cctgcatcac
    19741 atgaacaaac cctttatcac gactgatagc accattcgga cagacgttag cgcacggtgc
    19801 atcttcacac tgacggcaaa ctgtcgccgt ggaaatgttc acacctttaa tgacatggat
    19861 tcgcggtaaa aaagtttccg gggtcagcga tgcacagtcc tgattttcct gatgagaaac
    19921 cacgcacgcc acttcacagg tacggcaacc aatacattta ctcgcgtcag caatgatgaa
    19981 acggttcatc aaattctcca gcaatgacag ttaatgcgcc gatacattca caaatcatgc
    20041 cagtttttaa tttactgtta tttaaagaaa ttaaattctg taatgcagga aaaacgatgt
    20101 catcgacact agtgacgatg acatgtgatg acaatgttta tcgcgaaaga gcaatgaggg
    20161 agtcgcggcg gatcagtttt ccgctgaagg ttttcggcgg tgtgtaagca ccaccatcga
    20221 gcataaatat cagccgccca attatttcct gaatcatctc agtcaccgga atttttacgc
    20281 tggagagcgc cggaacggtg tagggggcaa tagcgatatc atcgaatccg ataactgaca
    20341 cctgctctgg caccgctacg ccgcgctcgt gcaacgcttt catcgcacct atcgccatat
    20401 cgtcgttact ggcaactaac gcgctaaatt tagccccacg ttcgagcaac gtttctaccc
    20461 cttcggcccc gctggcaggc gtccatttac cgttagcgat aagtttttca ttgaacgcaa
    20521 taccatgctg cgccagcgcg tctttatatc cggcaagacg ttcaatgctg gtgggggaat
    20581 ccatcgagcc ggtaaggaaa gcaatctcct gatgtccggc ggttatcaac tctgccacgg
    20641 cgttaaagct ggtctgttta tgatcgcacc agacgctatg gctgctgttt ttgcgcaggc
    20701 ggcgattaag caccattatc ggctgactgt gtgcgtcaat gatgtcatca atctcatcca
    20761 cgcttaaaaa gcgcgggtaa atcatgatgg cgtcgcagcg caaatccagc agatactgaa
    20821 tcgcctggcg ctcttcttcg gcgctatgtt taccatctgc caatagcaac tgtcgccctt
    20881 tctcttccgc cattcgcgcg gcatgaaaca gtaattcact aaaataaatg ccatggtaaa
    20941 gcgtgttggt cacgaccagc cccagcgtct gagtactctt cgccgacaga ttgcgcgcca
    21001 gcaagtttgg acggtaaccg ctctcttcta ccgcctgaaa cacgcgatct ttagtctcct
    21061 ggctgacgta gccattacct gaaagcacgc gggaaacggt cgcttttgaa accccggcgc
    21121 gcttcgccac ttccagcatc gtcgtcatca ttttcatccc tttacacgta atcaacgcag
    21181 tgtactgcac cgtttgccga ttgtccttgc gcaatcggcg ggaaaaatat tcaggtgacc
    21241 ggtttcacaa atataaaaaa tgaacaatta actctcttgc ttatttagtg acaactattc
    21301 atgattttgt gaaaccggtt tcttaattcc gtttcagcat cggcattttt ccgtcacgtc
    21361 gactgataac tactacatct accctactga taacaggata aaatccgatg gccaaaaatt
    21421 atgcggcgct ggcacgctcg gtgatagcgg cactgggcgg cgttgataac atctcggcag
    21481 tcacgcactg tatgacgcgt ttgcgctttg ttatcaaaga tgaccagctt atcgacagtc
    21541 cgacgttgaa aaccatctcc ggcgtgctcg gcgtggtacg tagtgacaac cagtgtcagg
    21601 tgattatcgg caataccgtt tcacaagcct ttcaggaagt cgtcagcctg ctgccgggag
    21661 atatgcagcc cgcacagccc gtgggtaaac ccaaactcac gctacgtcgc attggtgcgg
    21721 ggatcctcga tgcgctgatc ggcaccatgt caccgctgat cccggcgatt atcggcggat
    21781 cgatggtcaa actgctggca atgatcctcg agatgagcgg cgtgctgaca aaaggatcgc
    21841 cgaccttaac cattctgaat gtgattggtg acggtgcttt cttcttcctg ccgctgatgg
    21901 tcgctgcttc tgccgccatc aaatttaaaa ccaatatgtc gctggcgatt gccatcgcgg
    21961 gtgtgctggt acatccgagt tttattgagc tgatggcgaa agcggcccag ggtgaacatg
    22021 ttgaatttgc cctgattccg gtcaccgcgg tgaaatacac ctacacagtg atcccggcgc
    22081 tggtgatgac ctggtgcctg tcatatatcg aacgctgggt ggacagcatt acgccagcgg
    22141 tgacgaaaaa cttcctcaag ccgatgctga ttgtgttgat tgccgcaccg ctggcaatcc
    22201 tgctgattgg cccgattggt atctggatcg gtagcgccat ttcggcgctg gtttacacca
    22261 ttcatggtta tctgggctgg ctttcagtcg ccattatggg cgcactgtgg cctctgctgg
    22321 taatgaccgg gatgcaccgc gtctttacgc caaccatcat tcagaccatt gccgaaaccg
    22381 gcaaagaagg tatggtcatg ccgtcagaaa tcggcgctaa cctgtcgctg ggcggttcat
    22441 cgctggcggt ggcgtggaaa acaaaaaacc cggaactgcg ccagacggcg ctggctgcgg
    22501 cggcatcagc cattatggcg gggatttccg aaccggcgtt atacggcgtg gcgatccgcc
    22561 tgaaacgtcc gcttatcgcc agtcttatca gcggttttat ttgcggcgcg gttgccggta
    22621 tggcggggct tgccagccac tcaatggcag cgccggggct atttaccagc gtgcagttct
    22681 tcgatccggc gaatccaatg agcatcgtct gggtgttcgc cgtcatggcg ctggcggtcg
    22741 tgctgtcgtt catcctcaca ctgttgctcg gctttgagga tattcctgtt gaggaagcga
    22801 ctgccgaggc gcgaaagcat cagagcgcac aaccgaccgt cgccaaagaa gtaagtctta
    22861 attgaggatg aaaatgtcag tatttccaga aggtttttta tggggcggcg cgcttgccgc
    22921 caaccagtct gaaggtgcgt accgggaagg tggcaaaggg ctgaccaccg tcgatatgat
    22981 cccacacggc gagcatcgaa tggcggtgaa actggggctg gaaaaacgtt ttcagttgcg
    23041 cgatgacgag ttttatccca gccatgaggc gacggatttt tatcatcgtt ataaagaaga
    23101 tatcgccctg atggcagaga tgggattcaa agtgttccgt acctcaattg cctggagccg
    23161 cctcttcccg cagggcgatg aactgacgcc caaccagcag ggcattgctt tttaccgttc
    23221 tgtctttgaa gagtgtaaaa agtacggtat cgaaccgctg gtcacgttgt gccacttcga
    23281 tgtgccgatg catctggtca ccgaatatgg ctcctggcgt aaccgcaagc tggtggagtt
    23341 tttcagccgc tacgcccgga cctgctttga agcatttgat ggtctggtga aatactggct
    23401 caccttcaat gaaatcaaca ttatgttgca tagcccgttc tccggcgcgg gtctggtgtt
    23461 tgaagaaggc gaaaatcagg atcaggtgaa atatcaggct gcgcatcacc aactgattgc
    23521 cagtgcatta gccaccaaaa tcgcccatga ggttaacccg caaaatcagg tgggctgtat
    23581 gctggcgggc ggtaacttct acccttacag ttgcaagccg gaagatgtct gggcggcgct
    23641 ggagaaagat cgggaaaacc tgttttttat cgatgtgcag gcgcggggcg cgtatccggc
    23701 ttactctgcc cgcgtattcc gcgaaaaagg ggtaaccatc gacaaagcac cgggcgatga
    23761 tgagatcctg aaaaacaccg tcgattttgt ctctttcagc tattacgcct cacgctgcgc
    23821 ctcggcggag atgaacgcca acaacagcag tgcggcgaac gtggtgaaat cgctgcgtaa
    23881 cccatatcta caggtaagcg actggggctg ggggattgat ccactcggtc tgcgtatcac
    23941 catgaacatg atgtatgacc gctatcagaa gccgctgttt ctggtggaaa acggcctcgg
    24001 cgcaaaagat gaacttgctg ccaatggcga gattaacgat gactatcgca tcagctattt
    24061 acgcgaacat atccgcgcaa tgggcgaagc gattgcagat ggcattccgc tgatgggcta
    24121 caccacttgg ggctgtattg atttggtttc cgcctcaacg ggtgaaatga gcaaacgcta
    24181 cggttttgtc tacgtagacc gtgacgacgc aggcaacggt acgctggcgc gcacgcgtaa
    24241 gaaatcgttc tggtggtata aaaaagtgat tgccagtaat ggtgaggatt tagagtaagt
    24301 aacagtgccg gatgcggcgt gaacgcctta tccggcctac atgtcgcacg aaccaggtag
    24361 gcccgataag cgcagcgcat cgggcaatct ggcgtttgct atcagttctg attccctcat
    24421 caagaacatc cctgtcctga ttccttactg aaaaactact cttcttccac cgctaactgc
    24481 gcgaatccgc cattcccttc ccagccttcc agtcgttgat aaacggtttc taccgcatct
    24541 ttaatcggct gggtcatcgg gtagtaaaag ccgacgatat ccggctggat gccgaggaaa
    24601 atcacttcgc caatgtcttc ttttaactgg tcgataaggt agttcaacgg catgttatgg
    24661 gtagtcatca taaacatctc ggcgatatca tccggatcga tgatgcggat ctcgccgggg
    24721 tttagcccca tatccgtggc gtcgacaatc agcagtcgtg tcgggcgcag ttcgcgaata
    24781 gcgacaatgt cgttttccgg tgcactaccg ccgtcaatca ccacccagtg acctttcggc
    24841 gcggcggcgc acttttccgc cagtagcggg cctgcgccat catcgcccat catgctattg
    24901 ccaacacaga gtaaaacgtc agtcacgcaa tctcctcacc atcaaataga tggcgttttc
    24961 ctgatgaata tcatgcagca tacttaacag cattgtactc cattccttct gctgcgaagt
    25021 ttgcgctgcc cgggctttat cgaatgcgtt ggccagcatc ggcacatggt taatgtcgat
    25081 gacgatctca ccatatttcg gcacgccttc cattttgcgg cgggcttcgc tgcccgcctc
    25141 cagagtggca atccatgcca gatattcatc ccacggacag gtgagcgccg cttccaggca
    25201 atcgataacc ccaaggtggt gaccaatcgc caggctgtaa tagaccacct gctgcgcctc
    25261 ggcgggcgtg gcatcgttct catcaataaa tttacggctc agttgactga acaccacctt
    25321 ttcactcatc ggatacgcgc ctcttcaaca acatgattca gatggctgac aatctcgttc
    25381 agacgcggat cgttttccgc ttccagccag cgtgcaacct gctcttcgcc ctgccctaac
    25441 tgtgtcagga agtcatcggc aatctgacga ccgtaacgat aacctgccag gcggcgtgct
    25501 tcgcgatcca ctttcacgcg cagcggctgc accatatcgc catgcaggat ctccgccggt
    25561 tgttcatcct gctcacccgg cccacgggcg tggattttct gctccagcag gccgagcgcc
    25621 atcgcaaagc cgtacagcgt ggcggcaggc gttggcgggc agccagggat ataaacatcc
    25681 actggaacga ttttatccgt accgccccac acgcagtaga gatcgtggaa gatcccgccg
    25741 ctgttaccgc aggcaccgta agagatacag attttcgggt ccggcgcgga ctgccacgca
    25801 cgcagcgcag gggatcgcat tgcacgggtg accgcgccgg taaacagtaa aatatccgca
    25861 tgacgcggtg acgggacgac tttaatgccg aagcgttctg catcgaacag cggcgaaagc
    25921 gtggcgaaaa tttcgatttc gcagccgttg cagccgccgc agtccacgcg gtaaacgtag
    25981 gcagaacgtt tgattttttt cagtaacgac gccttcatgc tggcgatgga ttcatccacc
    26041 gtcatcggga ccggaatgcc gttggcgtca cgggggccta ataaattgct catcagatgg
    26101 cctctttcat atggcgagtc agttcaatac ggtcggacgg caccaggcat ttctggcgct
    26161 tacattccgg gcaagtctca aagctttcgc ggtggttttc cgcgcggctg tcgccgttgt
    26221 gcttaagcag cgcaatggcg tagtcgatct ctttctggac ggcgaaagga cgattgcaga
    26281 cgcggcagtt gcacagcgcg aagcgggact gttggaggaa gtcttctttc ttccacaccg
    26341 ccagttcgta ctcttgcgac agtttgatcg ccgccgtcgg gcaaacttct tcgcagcgtc
    26401 cacagaagat gcagcgcccg agattaaact cccaggccag ctctcctgtg gcgaggtcgg
    26461 tttcaaccgt taaggcgttt gacgggcagg cattgacgca ggccgcgcag ccgatgcact
    26521 gctgcgggtt ctgctctggc ttaccacgga agtttttatc aaccgcaatc ggctccagcg
    26581 gataagacga ggtcgccgtg ccggttttga tgactttttt gataaaggta aacatggcga
    26641 ttccttattt cagcggcgag tttttacgct caatgctgta acgctcgagt tctttgtacg
    26701 gcaccacttt gctcttcttc ttacgcacat cgaccacggt catgcggtcg gtacaggagt
    26761 agcaagggtc caggctaccg ataatcagcg gcgcatcgga aacggtgttg ccgcgcagca
    26821 tgtagcgcag ggtcggccag ttcgcgtagg tcgcggcacg gcagcgccag cggtacagct
    26881 tctggttgtc gccggtcatg ctccagtgga tatcgtcgcc gcgcggcgct tcggcaaagc
    26941 ccagcgcaaa acggtgcgga atgtaggtaa aaccttctac catcaacgga ccgcccggca
    27001 ggttatccag accgtagtcg atcatgttca gcgcggtata gacctcgttg attcgcactt
    27061 tcagacggga aataacgtcg cagccctgct cgctgtggac ttccattggc agcaggccat
    27121 aaccgacaaa cgggtgatcg gcgcgagtat cgcgggcgtg accgctggcg cgaaccatcg
    27181 ggccaacgtt actgaaatcg cgggcgattt ccgggtccaa acgaccaatg ccgacggtgc
    27241 gctgttccat gttcggtgtg ctcagcagca catccaccag ctcctgcact tcacgacgca
    27301 tctgttgtgc cagctggcgg gtctggatca tgtcgtcttt cagcagatcg cgacgaatcc
    27361 cgccgatcag gttcaggccg taggttttac gtgcaccggt aaggatctct gccattttca
    27421 tggaggtttc acgcacgcgg aagaactgca taaagccgga gtcgaagccg gtaaagtgac
    27481 aggcgaggcc gaggttgagc agatgcgagt gcaggcgttc cacctccaac agaatggcgc
    27541 ggatcatctg cgcacgttct ggcacctgaa tacccatcgc gttttccacc gacgtggtgt
    27601 aggcggtgct gtgagcaaag ccgcagatcc cgcacacacg gtcagagagg aaggtcactt
    27661 cgttgtaacc catgcgggtt tccgccagtt tttccatgcc gcgatggaca tagaacaggc
    27721 ggtagtcggc gtcgataatg ttttcgccat cgacgaacag acggaagtga cccggttcgt
    27781 cggaagtaac gtgcagcgga ccaatcggca cgacgttgtt tttcttgtcg cccagttcgt
    27841 tgatgaactc gtaggtttca gcatcggtag tcggtgccgg acgttgacga taatccatgc
    27901 tgtctttacg cagcggataa agttcatccg gccagtcatc cggcagcacc agacgacgtt
    27961 catccggcag ccctaccgga atcaaaccgt acatatcgcg cacttcacgc tcgccccata
    28021 ccgccgccgg aacgcgcggc gttacggacg gatattccgg tttgttggcg tcaacttcaa
    28081 cgcgcacggt tatccagcat ttggtgccct tctccatcga cagcacgtaa taaacggcgt
    28141 aatgaccatt cagtttacgt tcgtcgttac caaacagcac cgacagccag ccaccctgct
    28201 tgtaatagag aaactccact acttccggca gatagttcac ctttacggtg acagtcagct
    28261 gatctttggt ctgccaggcg tggtccagca cgacgccagg aaatgcctca ttcagcgcgg
    28321 cgagataatg ttgacctaat ttttcttcag acatgctcaa actctcttta atcacgccgc
    28381 cagcaaggag acgaacgcta aaaatgcaaa gccaaacccg gcccaggtaa tgcgcggagt
    28441 aatatcaaga cgcagacggg ccatgctgtt ttcgaacagc gcgataacca ggacgccaac
    28501 caccagtttg acgatggcaa tcaccagcgc cagcagcagc ccaccgacgg tgaaggtttc
    28561 catttgtccc cacggaataa acaccccgac gaacatctgc aacaccacca gctgtttcag
    28621 gctgatgccc catttcatga cgccaaagcc gctgccgcta tactcagaga gcggaccttc
    28681 ctgcaactcc tgctcggctt ccgccaggtc gaacggcagt ttgcccattt cgataaaggt
    28741 ggcgaatgca caggcacaaa gcgccagcac cagcgggatg ctctggctca gcggccagtg
    28801 ataaacggtg tcggtgatgt tgctgatgtt ggtggaaccg gcaacctgcg ccgcaaccca
    28861 cagaccaagc agcagcatcg gttcaaccaa cacgccaagc atcgcttcac ggctcgcgcc
    28921 gatagcggta aacgggctac cggtatccag accagaaatg gcaaagaaga aacgcgcgat
    28981 ggcaaagaga tacagtaaag tgatcaaatc gcccagttgc ggcagcggag aaccgacggt
    29041 caccaccggc agcgcagtgg cgatagtcag catgacgccc accatcacat acggcgtcag
    29101 gcggaacacc cagccggagg catccgggcc aacgctctga cgccccagca gtttgataat
    29161 gtcgcgatac tcctgcaaca cgcccggccc acgacggtta tgcaagcggg cgcgcgctac
    29221 gcgggtaata ccggagagca gcggcgcaac ggcaaataac accagcgcct gaattaacgg
    29281 atataaaaca ctcattctca ggctcctcgt gaaacaataa tcaccaccag taccgccagt
    29341 tcaaccagcg ccatccggcg gaacagcaac gcactcccct cgcactgcca gcccggcacc
    29401 agcgacaccg gattcagcca tttgcgtagt ttcagcaccg gcgcaaacgc ctgtttcacc
    29461 ggcatggcaa aaccgtgagc ggtaatcacc attgattttt catgatcgta gccgcacacc
    29521 caggccgcgc cgcgggaacg cgacggcaaa cgatcgcctt tgcaaatcgc cataatgatg
    29581 aatggcagca gcgggcaggc aatcagcagc aacgtgatca tgggttgaga aacggtggtg
    29641 ttagcaggct ccagcggcag aggtacagca gcagagagca tcggcagtag ccacggcgca
    29701 gcaacaccgc caattacgca gcaaatcgcc agtgccacta cgcttacgct catcaggagc
    29761 ggcgcacagg tggcgttttc ggcctctttg gtacgcggcg cgccgaggaa agtaacgcca
    29821 taaactttcg ccatacacat caccgccagc gcaccggtaa ttgccagccc cacggcgagc
    29881 agcggcccga gaagacgggc aacaaacgcg ccactattgc tcagtttgaa gaaggattga
    29941 tagataaccc attccccggc aaacccgttc agcggcggca gcgcagccat tgccatcagc
    30001 ccgactaaca tggcgatgga gataaccggc attttcttgc caataccacc gagtttttcg
    30061 atatcgcgat gaccggtacg gaaccagacg ctccccgcac caaggaacag tacgctcttg
    30121 aacaggctat ggttaagcag atggtacaga ccaccaacca gaccaagggc aatcagcgcc
    30181 ggttgttcga gcgcgatacc cgttacgcca gcgcccagcc ccagcaggat gatgccgata
    30241 ttttccaggg tgtggtaagc cagcagacgt tggatattat gttccatcaa cgcatacaga
    30301 ccgccgacga atgcggtgat catgccgagt accagcagtg cgatccccca ccacagcggt
    30361 gcattaccgc ccagtagtga cagagttaaa atgcccagca ggccaatttt catgactacc
    30421 gtggaaaaca gcgcggcagc tggcgcagag gcgttggcat gtgcctgtgg cacccagccg
    30481 tgcagcggaa taatcccggc cagcaggcca aagccaatca caccgagcag ccagatatcg
    30541 gaaccgagcg gcagctgttg catacgcata tccagcaggc gcagatccag cgtgccgtaa
    30601 cgctgccaca gcagccagca ggcaatcgcc agcagcagag tgccaagacg ccccagcgca
    30661 aaccacagtt tgccctcttt gctgttgctg gtgaggaaca ccgcacacag ggccatgatt
    30721 tcggccatta ccacgaacat gccgaggttg ctggcgatga cggcgcagac ggcggcagcc
    30781 atcaacatat tgatctgcaa gccgttgcac ttcacctgcg cgtggcgatg ccagtcaatg
    30841 ttgtagaggc tgacaaacag accgcatagg ccgagcgtaa tcagccaaat cgcattaagc
    30901 ggagagattt gcacatcgta gcttaccagc gacagcgcac cgctcacgcc aaccgcgcca
    30961 gtcagcactg tgaagcccgc ggctgccgta tacaggctac caaccgcgcc gccaattccg
    31021 gctatccagc cgcttagcgc cttttgaaaa gaaaagagaa atgccagaac agcggcggcg
    31081 acaaaccacg ccacgccgct attgatcagg gaaattgcgc tcatttagcc tctccacttt
    31141 gagcctgctg aaacaaggtg agatcgccaa aatccgtgtt aaaagtcagc tcacgcttac
    31201 gtttgctgac gcgggcgata tcggtgttat ccaccagatg cagggcttta gtcgggcaca
    31261 tccgcacgca ggccggacct tgttcatcaa agctacaaag gtcacatttg acggcgatcg
    31321 cgcgaatacc tggcacccag tcaagcaatg tgctgacacg cgccggagca ggcggtgccg
    31381 gtggcgcttt cggggtattg gcgtttgccg gaatatccag cggacggctg ccggaaaatt
    31441 caattgcgcc aaacgggcag gcgatgccgc acagtttgca gcttacgcac agactttcat
    31501 tcaactgcac ggccccatca acgcgggtga tggcgttaac cgggcaaacc accgcacagg
    31561 gcgcatcttc acagtggtga cagagctgcg gcgcagattc tttttcattg agcatcactc
    31621 gcaggcgcgg cattgattgc aggccgtgct ggcgatgcgt ctctgaacag gcggcctcac
    31681 aggtgtggca gccgatacag agcgtggagt cagcaattac aaaacgattc accaggcatt
    31741 cctcaggtga ttgtcatttt tgacgaaaac atgccgatga aatgtcattt tcgacactca
    31801 tcgacacgcc catccccaaa caggcgtaac gcctgcaaaa cgggcaaagc ctcagctcat
    31861 gctgccgggc tttgtccctt taccagttgg cttaaattca ccggcacatt gttttcaatg
    31921 gcggtgtata agctgtcgta aacgccagcg attttttcga gatagtgttc cagaacctgt
    31981 tcgcgatcgc ggatgttgac cttgccatca atcatgccgt caatgctcag ttgcgcctgc
    32041 gcaatccgct gtttgtcttc accatctttt gtttcgacgt aatactcgat ggtgtggctg
    32101 ttaaagccat ccgccagggt gacgtaaata cgaaaatgtt caaaaaggac gaaacagagt
    32161 tctttgtccg gcgcgcagct catggcgtga tgcaggcgcg ctttcgataa cgtgattccc
    32221 tgaaccagcg aattgcagta gatgtgccac tggtcctgta ggcgacgatg ccgctgtgcg
    32281 atgtaatcgg ctttctcgct tatttcccaa atagtcattg tcaggttacc cgtttaacag
    32341 agatgccagc tttaagcatt ttttgtgcca acttttaatt tattgttatt aaagcgattt
    32401 ttaagctaaa gatgaatttc gtcgccgtgt cgacgtgtca tttcgacatc atcgacatta
    32461 ttcaccgcag ggataatcaa cactggcaca attattgctt gtagctggca atagttaatg
    32521 ggaggcgata tgcacgaaat aaccctctgc caacgggcac tggaattgat cgaacagcag
    32581 gccgcaaaac acggcgcaaa acgcgtaact ggggtctggc tcaaaattgg cgcattttct
    32641 tgtgtcgaaa ccagctctct tgccttttgt tttgatctgg tttgccgcgg cagcgtggcg
    32701 gaaggttgta aactgcacct cgaagaacaa gaggccgaat gctggtgtga aacatgccaa
    32761 cagtatgtga cgctactgac ccagcgcgtc cgccgctgtc cacagtgtca tggtgacatg
    32821 ctacagattg tggcagacga cggtttacag attcggcgga tagaaataga ccaggagtga
    32881 gcgatgtgta caacatgcgg ttgcggtgaa ggcaacctct atattgaggg tgatgaacat
    32941 aaccctcatt ccgcgtttcg tagcgcgcca tttgccccgg cggcacgccc gaagatgaaa
    33001 atcaccggca ttaaagcgcc tgaatttacc cccagccaga ctgaagaagg cgacctgcat
    33061 tacggtcatg gcgaagcggg cactcacgcg ccgggcatga gccagcgtcg gatgctggaa
    33121 gtcgaaattg acgtgctgga caaaaataac cgtctggctg aacgcaaccg cgcgcgcttt
    33181 gctgcccgca agcaactggt gctcaacctg gtttccagcc ctggttccgg taaaaccacc
    33241 ctgctgacgg aaactttaat gcgcctgaaa gacagcgttc cgtgcgcggt tattgaaggt
    33301 gaccagcaaa ccgtgaacga tgccgcacgc attcgcgcta ccggcacccc agcgattcag
    33361 gtgaacaccg gtaaaggctg ccatcttgac gcacagatga ttgccgacgc cgcgccgcgc
    33421 ctgccactgg acgataacgg tattctgttt atcgaaaacg tcggtaacct cgtttgcccg
    33481 gccagcttcg atctcggtga aaaacacaaa gtggcggtgc tttccgttac cgaaggtgaa
    33541 gacaaaccgc tgaaatatcc gcacatgttt gccgccgcct cgctgatgct gctcaacaaa
    33601 gttgacttgc tgccgtatct caatttcgac gttgagaagt gcatcgcctg cgcccgtgaa
    33661 gtcaatccag aaattgaaat catccttatt tccgccacca gcggcgaagg gatggaccag
    33721 tggctgaact ggctggagac acagcgatgt gcataggcgt tcccggccag atccgcacta
    33781 ttgacggcaa ccaggcgaaa gtcgacgtct gcggcattca gcgcgatgtc gatttaacgt
    33841 tagtcggcag ctgcgatgaa aacggtcagc cacgcgtggg ccagtgggta ctggttcacg
    33901 ttggctttgc catgagcgta attaatgaag ccgaagcacg cgacactctc gacgccttac
    33961 aaaacatgtt tgacgttgag ccagatgtcg gcgcgctgtt gtatggcgag gaaaaataat
    34021 gcgttttgtt gatgaatatc gcgcgccgga acaggtgatg cagttaattg agcatctgcg
    34081 cgaacgtgct tcacatctct cttacaccgc cgaacgccct ctgcggatta tggaagtgtg
    34141 tggcggtcat acccacgcca tttttaaatt cggcctcgac cagttgctac cggaaaacgt
    34201 tgagtttatc cacggtccgg gttgcccggt gtgcgtactg ccaatgggta gaatcgacac
    34261 ctgcgtggag attgccagcc atccggaagt catcttctgt acctttggcg atgccatgcg
    34321 cgtaccaggg aaacaggggt cgctgttgca ggcaaaagca cgcggtgccg atgtgcgcat
    34381 cgtttactcg ccaatggatg cgttaaaact ggcgcaggag aatccaaccc gtaaagtggt
    34441 attcttcggc ttaggttttg aaaccaccat gccgaccacc gccatcactc tgcaacaggc
    34501 gaaagcccgc gatgtgcaga atttttactt cttctgccag cacattacgc ttatcccgac
    34561 gctgcgcagt ttgctggaac agccagataa cggtatcgac gcgttcctcg cgccgggcca
    34621 cgtcagtatg gttatcggca ctgatgccta taattttatc gccagcgatt ttcatcgccc
    34681 gctggtggtc gctggtttcg aaccccttga tctactgcaa ggcgtggtga tgctggtgga
    34741 gcagaaaata gcggcccaca gcaaggtaga gaatcagtat cgtcgggtgg taccggatgc
    34801 cggtaacctg ctggcgcaac aggcgattgc cgatgtgttc tgtgtcaacg gcgacagtga
    34861 atggcgcggc ttaggcgtga ttgaatcttc tggcgtgcac ctgacgccgg attatcaacg
    34921 attcgatgcc gaaacacatt tccgcccggc accgcagcag gtctgcgatg acccgcgtgc
    34981 gcgttgtggc gaagtcttga cgggcaaatg taagccgcat caatgcccgc tgtttggtaa
    35041 cacctgtaat cctcaaaccg cgtttggtgc gctgatggtt tcctccgaag gagcgtgcgc
    35101 cgcgtggtat cagtatcgtc agcaggagag tgaagcgtga ataatatcca actcgcccac
    35161 ggtagcggcg gccaggcgat gcagcaatta atcaacagcc tgtttatgga agcctttgcc
    35221 aacccgtggc tggcagagca ggaagatcag gcccgtcttg atttggcaca gctggtagcg
    35281 gaaggcgacc gtctggcgtt ctcaaccgac agttacgtca ttgacccgct gttctttcct
    35341 ggcggtaata tcggcaagct ggcgatttgc ggcaccgcca atgacgttgc ggtcagtggc
    35401 gcaattccgc gctatctctc ctgtggcttt atcctcgaag aaggattgcc gatggagaca
    35461 ctgaaagccg tagtgaccag catggcagaa accgcccgcg cggcaggcat tgccatcgtt
    35521 actggcgata ccaaagtggt acagcgcggc gcggcagata aactgtttat caacaccgct
    35581 ggcatgggcg caattccggc gaatattcac tggggcgcac agacgctaac cgcaggcgat
    35641 gtattgctgg ttagcggtac actcggcgac catggggcga ctatccttaa cctgcgtgag
    35701 caactagggc tggatggaga actggtcagc gactgcgcgg tgctgacgcc gcttattcag
    35761 acgctgcgtg acattcccgg cgtgaaagcg ctgcgtgatg ccacccgtgg tggtgtaaac
    35821 gcggtggttc atgagttcgc ggcagcctgc ggttgtggta ttgaactttc agaagcggca
    35881 ctgccggtta aacctgctgt gcgcggcgtt tgcgaactgc tgggactgga cgcccttaac
    35941 tttgccaacg aaggcaaact ggtgatcgcc gttgaacgca acgcagcaga gcaagtgctg
    36001 gcggcgttac attcccatcc attgggaaaa gatgcggcgc tgattggcga agtagtagaa
    36061 cgtaaaggtg ttcgtcttgc cggtctgtat ggcgtgaaac gaaccctcga tctaccgcac
    36121 gccgaaccgc ttccgcgtat atgctaataa aattctaaat ctcctatagt tagtcaatga
    36181 ccttttgcac cgctttgcgg tgctttcctg gaagaacaaa atgtcatata caccgatgag
    36241 tgatctcgga cagcaagggt tgttcgacat cactcggaca ctattgcagc agcccgatct
    36301 ggcctcgctg tgtgaggctc tttcgcaact ggtaaagcgt tctgcgctcg ccgacaacgc
    36361 ggctattgtg ttgtggcaag cgcagactca a
//
LOCUS       DAPHCV010000044        35845 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.044, whole genome shotgun sequence.
ACCESSION   DAPHCV010000044 DAPHCV010000000
VERSION     DAPHCV010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.044"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1914
                     /gene="flhA"
                     /locus_tag="RH033_002138"
     CDS             <1..1914
                     /gene="flhA"
                     /locus_tag="RH033_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HDV2991318.1"
                     /translation="TQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYE
                     EFCETWPQLATGGLYPKFGHYCLMPLAAEGHIFGGCEFIRHDDRPWSEKEFNRLQTFT
                     QIVSVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIH
                     YYFDIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLL
                     INLHERDDLAPYERMLFNTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLN
                     LLRQIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMY
                     SVLKQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLE
                     SDLFGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERL
                     GSNKIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLA
                     KAFTFKIARRLGRNIDSIPAETLRILSNMEWPGNVRELENVIERAVLLTRGNVLQLSL
                     PDIALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLS
                     RMKRLGIDKSALI"
     gene            1959..2387
                     /locus_tag="RH033_002139"
     CDS             1959..2387
                     /locus_tag="RH033_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012134844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HDV2991319.1"
                     /translation="MAVSARNQLTGTVSAVAMGAVNDEVELTLAGGAKLVAIVTHSSQ
                     QALGLAKGKEAIALIKAPWVTLATEDCGLKFSARNQFAGSVSTITEGAVNATVHIKTD
                     AGFEIVAVVTNESQDEMKLTTGSRVIALIKASAILIATKA"
     gene            complement(2426..2770)
                     /locus_tag="RH033_002140"
     CDS             complement(2426..2770)
                     /locus_tag="RH033_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HDV2991320.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKK"
     gene            complement(2825..2980)
                     /locus_tag="RH033_002141"
     CDS             complement(2825..2980)
                     /locus_tag="RH033_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2991321.1"
                     /translation="MIPELIISGEEANGEFLMVIPGKMLFCSFCVMTMKQDRKIRRLK
                     KPPPSET"
     gene            3056..5617
                     /gene="mutS"
                     /locus_tag="RH033_002142"
     CDS             3056..5617
                     /gene="mutS"
                     /locus_tag="RH033_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDV2991322.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMERQQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLEGVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIATGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            5723..6379
                     /gene="pphB"
                     /locus_tag="RH033_002143"
     CDS             5723..6379
                     /gene="pphB"
                     /locus_tag="RH033_002143"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HDV2991323.1"
                     /translation="MPSTRYQKINAHHYHHIWAVGDIHGDYQLLQSRLHQLSFCPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDTIKYVIAHADYPGKEYQFGKEIAERELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(6430..7197)
                     /locus_tag="RH033_002144"
     CDS             complement(6430..7197)
                     /locus_tag="RH033_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDV2991324.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGRCIYLDAGT
                     TTLAIAQHLIHMEPLTVVTNDFVIADYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSMRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKLDLSLLIAKNE"
     gene            7393..8301
                     /locus_tag="RH033_002145"
     CDS             7393..8301
                     /locus_tag="RH033_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDV2991325.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNATQVKQVLFGGKGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGSEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            8298..9560
                     /locus_tag="RH033_002146"
     CDS             8298..9560
                     /locus_tag="RH033_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HDV2991326.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            9557..10195
                     /locus_tag="RH033_002147"
     CDS             9557..10195
                     /locus_tag="RH033_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDV2991327.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNIPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            10200..10976
                     /locus_tag="RH033_002148"
     CDS             10200..10976
                     /locus_tag="RH033_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HDV2991328.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSPQQ
                     IQQQLAQNHLTLALFNTAPGDINAGEWGLSALPGREHEARADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADHFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            11065..12429
                     /locus_tag="RH033_002149"
     CDS             11065..12429
                     /locus_tag="RH033_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HDV2991329.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(12467..12703)
                     /locus_tag="RH033_002150"
     CDS             complement(12467..12703)
                     /locus_tag="RH033_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2991330.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWMVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(12714..14141)
                     /locus_tag="RH033_002151"
     CDS             complement(12714..14141)
                     /locus_tag="RH033_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDV2991331.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFIDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRSKPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARQ"
     gene            complement(14141..14734)
                     /locus_tag="RH033_002152"
     CDS             complement(14141..14734)
                     /locus_tag="RH033_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDV2991332.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSTWAKTTIE
                     LETPYSARDVAALADFSHNPADQAATISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQVRNFSQENE"
     gene            14881..15288
                     /locus_tag="RH033_002153"
     CDS             14881..15288
                     /locus_tag="RH033_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDV2991333.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDAADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMSK"
     gene            complement(15409..16401)
                     /gene="rpoS"
                     /locus_tag="RH033_002154"
     CDS             complement(15409..16401)
                     /gene="rpoS"
                     /locus_tag="RH033_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HDV2991334.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(16464..17603)
                     /gene="nlpD"
                     /locus_tag="RH033_002155"
     CDS             complement(16464..17603)
                     /gene="nlpD"
                     /locus_tag="RH033_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HDV2991335.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(17743..18369)
                     /gene="pcm"
                     /locus_tag="RH033_002156"
     CDS             complement(17743..18369)
                     /gene="pcm"
                     /locus_tag="RH033_002156"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HDV2991336.1"
                     /translation="MVSRRVQALLDQLRAQGIQDELVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(18363..19124)
                     /gene="surE"
                     /locus_tag="RH033_002157"
     CDS             complement(18363..19124)
                     /gene="surE"
                     /locus_tag="RH033_002157"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HDV2991337.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(19105..20154)
                     /gene="truD"
                     /locus_tag="RH033_002158"
     CDS             complement(19105..20154)
                     /gene="truD"
                     /locus_tag="RH033_002158"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HDV2991338.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVMEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAEETELQTLLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(20151..20630)
                     /gene="ispF"
                     /locus_tag="RH033_002159"
     CDS             complement(20151..20630)
                     /gene="ispF"
                     /locus_tag="RH033_002159"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDV2991339.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYALGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(20630..21340)
                     /gene="ispD"
                     /locus_tag="RH033_002160"
     CDS             complement(20630..21340)
                     /gene="ispD"
                     /locus_tag="RH033_002160"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDV2991340.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKHVVIAISPGDSRFAQLPLANHPQITVVDGGEERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAETGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(21359..21670)
                     /gene="ftsB"
                     /locus_tag="RH033_002161"
     CDS             complement(21359..21670)
                     /gene="ftsB"
                     /locus_tag="RH033_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HDV2991341.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNNDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(21864..22187)
                     /locus_tag="RH033_002162"
     CDS             complement(21864..22187)
                     /locus_tag="RH033_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HDV2991342.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(22237..22842)
                     /gene="cysC"
                     /locus_tag="RH033_002163"
     CDS             complement(22237..22842)
                     /gene="cysC"
                     /locus_tag="RH033_002163"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HDV2991343.1"
                     /translation="MALHDENVVWHSHPVTPQQREQHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(22842..24269)
                     /gene="cysN"
                     /locus_tag="RH033_002164"
     CDS             complement(22842..24269)
                     /gene="cysN"
                     /locus_tag="RH033_002164"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HDV2991344.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFSGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(24271..25179)
                     /gene="cysD"
                     /locus_tag="RH033_002165"
     CDS             complement(24271..25179)
                     /gene="cysD"
                     /locus_tag="RH033_002165"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HDV2991345.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            25431..26468
                     /gene="iap"
                     /locus_tag="RH033_002166"
     CDS             25431..26468
                     /gene="iap"
                     /locus_tag="RH033_002166"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HDV2991346.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEVFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   26550..27188
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=26611..26639
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
     gene            complement(27285..27578)
                     /gene="cas2"
                     /locus_tag="RH033_002167"
     CDS             complement(27285..27578)
                     /gene="cas2"
                     /locus_tag="RH033_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HDV2991347.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQISQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(27575..28498)
                     /gene="cas1e"
                     /locus_tag="RH033_002168"
     CDS             complement(27575..28498)
                     /gene="cas1e"
                     /locus_tag="RH033_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
                     /protein_id="HDV2991348.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSSKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRGG"
     gene            complement(28495..29145)
                     /gene="cas6e"
                     /locus_tag="RH033_002169"
     CDS             complement(28495..29145)
                     /gene="cas6e"
                     /locus_tag="RH033_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HDV2991349.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDIFTIECRPFAPELRTGQSLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQVEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            complement(29127..29873)
                     /gene="cas5e"
                     /locus_tag="RH033_002170"
     CDS             complement(29127..29873)
                     /gene="cas5e"
                     /locus_tag="RH033_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HDV2991350.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSAPD
                     LLSAIISRRDYYTDAWWMVAVATTPDAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNASDVLRNAYQQYQDSFRELKVSLPKLQDECWWEGEHDGLVASKILRRRDV
                     PLNRQQWLFGERTINQGPWLSKEEPCTSQE"
     gene            complement(29884..30939)
                     /gene="cas7e"
                     /locus_tag="RH033_002171"
     CDS             complement(29884..30939)
                     /gene="cas7e"
                     /locus_tag="RH033_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HDV2991351.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTLRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTEQPRSLAAAFYEPINGTLQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(30954..31490)
                     /gene="casB"
                     /locus_tag="RH033_002172"
     CDS             complement(30954..31490)
                     /gene="casB"
                     /locus_tag="RH033_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HDV2991352.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGNRASLRRSTTVNDVCLSEGF
                     RSLLMQTHTLWKIESQEWRFTALALVAAVAANVKAIDERQPFAAQLAAVMSEGRFTRL
                     SAVKTPDELLRQLRRAVKLLNGSVNLISLADDIFRWCQESDDLLNHHRRQQRPTEFIR
                     IRWALEYYQAGDADNEQN"
     gene            complement(31487..33049)
                     /gene="casA"
                     /locus_tag="RH033_002173"
     CDS             complement(31487..33049)
                     /gene="casA"
                     /locus_tag="RH033_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HDV2991353.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVHLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKDHRRWDDIWEDGLEAEKLREALLPLEHAFQFGPDSPSFM
                     QDFEALTGDKVPVASLLPEIPGVQTTKFNKDHFIKRGVTEHLCPQCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKMWLNVMPQDEADLPLPKKFDG
                     LVFPWLGPTRTSELAGAVVTDDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQNDA
                     LLSLMTTKNYGANYAMWQHPLTPYRIPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGVRGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLGKWQKEIWLFARQDFDERVFTNPYEPVDLKRVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(33147..35804)
                     /locus_tag="RH033_002174"
     CDS             complement(33147..35804)
                     /locus_tag="RH033_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDV2991354.1"
                     /translation="MTFFYFWGKTRRGEKDGGEDYHLLCWHSLDVAAMGYLMVKSNCF
                     GLAGYFRQLGFADTELAAQFFAWLLCWHDIGKFARSFQQLYLHPQLKVPEGARKNYEK
                     ISHSTLGYWLWNHYLSECQELLPSSSLSPRKLKRVMEMWMPMTTGHHGRPPERMDELD
                     NFLPEDKGAARDFLLAIKVLFPLIEIPTFWDDDEGVELIKQLSWYISATVVLADWTGS
                     STRYFPRVAQAMDIEDYWQKTLVQAQNALTVFPPKAESAPFTGINTLFPFIENPTPLQ
                     QKVLDLDISQPGPQLFILEDVTGAGKTEAALILAHRLMAAGKAQGLFFGLPTMATANA
                     MYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGSEEPDEQTFSQGC
                     AAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHACDAYM
                     SCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGIEGQQEAPLLEKDDYPWL
                     THVTKSDVHSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQGKCIAWIRNSVDDA
                     IQVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKEDCSQRAGKVLICTQ
                     VLEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGLLKRDGKDERSPPELLILA
                     PVWDDSPGDEWFGSAMRNSAYVYPDHGRIWLTQRVLREQGAIQMPHAARLLIESVYGE
                     DVAMPEGFARSEQEQVGKYYCDRAMAKKFVLNFKPGYAANINDYLPEKLSTRLAEESV
                     SLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEGDAFRQWCIEQRQ
                     DPEMANVILVTDDESCGYSAMEGLTGKVG"
BASE COUNT         8487 a         9654 c         9129 g         8575 t
ORIGIN      
        1 actcaacgtg cgtcttatta cgcatcgcgt gaaaaagaca cccccattaa atatgaagac
       61 gaaactgttc tggcacacgg tccggtacgc agcattttgt cgcgccctga tacgctgcat
      121 tgcagttacg aagaattttg tgaaacctgg ccgcagctgg ccacaggtgg gctatacccc
      181 aaatttggtc actattgcct gatgccactg gcggcggaag ggcatatttt tggtggctgt
      241 gaatttattc gtcatgacga tcgcccctgg agcgaaaaag agttcaatcg tctgcaaaca
      301 tttacgcaga tcgtttctgt cgtcaccgaa caaatccaga gccgcgtcgt taacaatgtc
      361 gactatgagt tgttatgccg ggaacgcgat aacttccgca tcctggtcgc catcactaac
      421 gcggtgcttt cccgcctgga tatggacgaa ctggtcagcg aagtcgccaa agaaatccat
      481 tactatttcg acattgacga tatcagcatc gtcttacgca gccaccgtaa aaacaaactc
      541 aacatctact ccactcacta tcttgataaa cagcatcccg cccacgaaca gagcgaagtc
      601 gatgaagccg gaaccctcac cgaacgcgtc ttcaaaagta aagagatgct gttgattaat
      661 ctccacgagc gggacgattt agccccctat gaacgcatgt tgttcaacac ctggggcaac
      721 cagattcaaa ccttgtgcct gttaccgctg atgtctggcg acactatgct gggcgtgcta
      781 aaactggcgc aatgcgaaga gaaagtcttt accactacca atctgaattt actgcgccag
      841 attgccgaac gtgtggcaat cgctgtcgat aacgccctcg cctatcagga aattcatcgt
      901 ctgaaagaac ggctggttga tgaaaacctc gccctgaccg agcagctcaa caatgttgat
      961 agtgaatttg gcgagattat tggccgcagc gaagccatgt acagcgtact taaacaagtt
     1021 gaaatggtgg cgcaaagtga cagtaccgtt ctgatcctcg gtgaaactgg tacaggtaaa
     1081 gagctgattg cccgcgcgat ccataatctc agtgggcgta ataatcgccg catggtcaaa
     1141 atgaactgcg cggcgatgcc tgccggattg ctggaaagcg atctatttgg tcatgagcgt
     1201 ggggctttta ccggtgccag cgctcagcgc attggtcgtt ttgaactggc ggataaaagc
     1261 tcactattcc tcgatgaagt gggcgatatg ccgctggagt tacagccgaa gttgctgcgt
     1321 gtattacagg aacaggagtt tgaacgtctc ggcagcaaca aaatcattca gacggacgta
     1381 cgtttaattg ccgcgactaa ccgcgatctg aaaaaaatgg tcgccgaccg agagttccgt
     1441 agcgatctct attaccgtct gaacgtattc ccgatccacc tgccgccact acgcgagcgt
     1501 ccggaagata ttccgctgct ggcgaaagcc tttaccttca aaattgcccg tcgtctgggg
     1561 cgcaatatcg acagcattcc tgccgagacg ctgcgcatcc tgagcaacat ggaatggccg
     1621 ggcaacgtgc gtgagctgga aaacgtcatt gagcgcgcgg tattgctaac acgcggcaac
     1681 gtgctgcagc tgtcattgcc agatattgct ttaccagaac ctgaaacgcc gcctgccgca
     1741 acggttgtcg cccaggaggg cgaagatgaa tatcagttga ttgttcgcgt gctgaaagaa
     1801 acaaacggcg tggttgccgg gcctaaaggc gctgcgcaac gtctggggct gaaacgcacg
     1861 actctgctgt cacgaatgaa gcgactggga attgataaat cggcattgat ttaacagatg
     1921 tggttatact cgatcaacat tcaatgaaag gaggcagtat ggcagtttca gcaagaaatc
     1981 aattaaccgg aacagttagc gcagtagcaa tgggcgcggt gaatgacgaa gtagaactga
     2041 cgctggcagg tggtgcaaag ctggtggcaa ttgtgactca cagcagccag caagcgctgg
     2101 gtctggcgaa agggaaagaa gcgattgccc tgattaaagc cccgtgggtc acgctggcaa
     2161 cggaagactg tggactgaaa ttctctgctc gcaaccagtt tgctggcagc gtgtcgacaa
     2221 tcaccgaagg cgcagtgaac gccactgtcc atatcaaaac ggatgcgggt tttgaaattg
     2281 tggcagtggt aacaaatgaa agtcaggatg aaatgaaact caccactggc tcgcgcgtta
     2341 ttgcattgat caaagcatca gccatcctga ttgccaccaa agcgtaagcg ccgtatttgc
     2401 cggagagtat ggctctccgg cagatttatt tctttggacg atatttttct ggcaactccg
     2461 gcaccggacg cttgtcatcg atgagatgac gcacggttaa gatcggatga cgccacagca
     2521 ttctcggccc ggcccaacgc ataatctgtt tcatctcttc acgctttgca ggctggtaac
     2581 agtgcaccgg acactgctta caggctggtt tctcttcgcc gaacacacat ttatccagcc
     2641 gcttttgcgc gtaaacaaac aacgcctcgt aatgctccgg ctccgctgac gcctgcgggc
     2701 atttcgcttg ataaagatcg atcatttttt taatcgtcag tttttcacga gagatacgct
     2761 tgccggacat gctgcctcca tactcttaag gtgcatttat attacaactt aattttaaag
     2821 gggactatgt ctctgaagga ggaggtttct tcaaccgacg aattttgcga tcttgtttca
     2881 tcgtcatcac gcaaaagctg caaaacagca tctttcccgg aatcaccatc aagaactcgc
     2941 cgttcgcttc ttcccctgaa atgattaact ccggtatcat gtgcgcctta tgtgattaca
     3001 acgaaaataa aaaccatcac accccattta atatcaggga accggacata accccatgag
     3061 tgcaatagaa aatttcgatg cccatacgcc catgatgcag cagtatctca agctgaaagc
     3121 ccagcatccc gagatcctgc tgttttaccg gatgggtgat ttttatgaac tgttttatga
     3181 cgacgcaaaa cgcgcatcgc aactgctgga tatttcactg accaaacgcg gtgcttcggc
     3241 gggagagccg atcccgatgg cggggattcc ctaccatgcg gtggaaaact acctcgccaa
     3301 actggtgaat cagggcgaat cggttgctat ttgtgaacag attggcgatc cggcgaccag
     3361 caaagggccg gtcgagcgca aagttgtccg tatcgttacg ccgggcacca tcagcgatga
     3421 agcgctgttg caggaacgtc aggacaacct gctggcggct atctggcagg acagcaaggg
     3481 gttcggctac gccacgctgg atatcagctc cgggcgtttt cgcctgagcg aaccggcgga
     3541 ccgcgaaacg atggcggcag aactgcaacg tacgaatcca gcggaattac tgtatgcgga
     3601 agatttcgcc gagatgtcgc tgattgaagg tcgccgcggc ctgcgtcgtc gcccgctgtg
     3661 ggagtttgaa atcgacaccg ctcgccagca attgaacctg caatttggca cccgagatct
     3721 ggtcggtttt ggtgtggaga acgcaccacg cggactttgt gctgccggtt gtctgttgca
     3781 gtatgcgaaa gatacccaac gcacgaccct gccgcatatt cgttctatca ctatggaacg
     3841 tcagcaggac agcatcatta tggatgccgc gacgcgtcgt aacctggaaa ttactcagaa
     3901 cctggccggc ggtgcggaaa atacgctggc ttcagtgctc gactgtactg taacgccgat
     3961 gggtagtcgt atgctgaaac gctggctgca tatgccagtg cgcgataccc gcgtgttgct
     4021 tgagcgccag caaactattg gcgcattgca ggatttcacc gccgagctgc agccggtact
     4081 gcgtcaggtc ggcgacctgg aacgtattct ggcgcgtctg gctttacgaa ctgctcgtcc
     4141 acgcgatctg gcccgtatgc gtcacgcttt ccagcaactg ccggagctgc gcgcgcaact
     4201 ggaaggtgtc gatagcgccc cggtacaagc actgcgtgag aagatgggcg agtttgccga
     4261 actgcgcgac ctgctggagc gagcaatcat cgacacgccg ccggtgctgg tacgcgacgg
     4321 tggtgttatt gcgactggtt ataacgaaga actggatgaa tggcgcgcgc tggctgacgg
     4381 cgcgaccgat tatctggagc gtctggaagt ccgcgagcgt gaacgtactg gcctggacac
     4441 cctgaaagtc ggctttaacg cggttcatgg ttattacatt cagatcagcc gtggacaaag
     4501 ccatctggca cccatcaatt acatgcgtcg ccagacgctg aaaaacgccg agcgctacat
     4561 tattccagag ctaaaagagt acgaagacaa agttctcact tcaaaaggca aagcactggc
     4621 actggaaaaa cagctttatg aagagttgtt cgacctgctg ttgccgcatc tggaagcgtt
     4681 gcaacagagc gcgagcgcgc tggcggaact cgacgtgctg gtgaacctgg cggaacgggc
     4741 ctatacgctg aactacacct gcccgacctt tattgataaa ccgggtattc gcatcaccga
     4801 aggccgccat ccggtggttg aacaggtgct gaatgagccg tttatcgcca acccgctgaa
     4861 cctgtcgccg cagcgtcgca tgttgatcat taccggtccg aatatgggcg gtaaaagtac
     4921 ctatatgcgc cagaccgcac tgattgcgct gatggcctac atcggcagct atgtaccggc
     4981 acaaaaagtc gagattggac ctatcgatcg catctttacc cgcgtagggg cagcggatga
     5041 tctggcatcc gggcgttcaa cctttatggt ggaaatgacc gaaaccgcca atattttaca
     5101 taacgccacc gagtacagtc tggtgctgat ggatgagatt gggcgtggaa cgtccactta
     5161 cgatggtctg tcgctggcgt gggcgtgcgc ggaaaatctg gcgaataaga ttaaagcgtt
     5221 gacgttgttt gctacccact atttcgagct gacccagtta ccggagaaaa tggaaggcgt
     5281 cgccaacgtg catctcgatg cactggagca cggcgacacc attgccttta tgcacagcgt
     5341 gcaggatggc gcagcgagca aaagctacgg cctggcggtt gcagctctgg ccggcgtgcc
     5401 aaaagaggtt attaagcgcg cacggcaaaa actgcgtgag ctggaaagca tttcgccgaa
     5461 cgccgccgcg acgcaagtgg atggtacgca aatgtctttg ctgtcagtgc cagaagaaac
     5521 gtcgcctgcg gtcgaagcac tggaaaatct cgacccagat tcactcaccc cgcgtcaggc
     5581 gctggagtgg atttatcgct tgaagagtct ggtgtaataa caattcccga tagtcttttg
     5641 ctatcgggaa tattaacgac aactgacgaa tcaaataaaa ataccctgta taataggaaa
     5701 tcttatttta cagggtaaaa ccatgccatc tacacgctac caaaaaatca atgcccatca
     5761 ctatcaccat atatgggccg ttggtgatat tcatggtgac tatcaattat tacaatcccg
     5821 cttacatcaa ctctcttttt gtcctgaaac cgacttgctt atttctgtcg gcgataacat
     5881 cgatcgtggg cccgagagtc ttaacgtgct gcgcctgtta aaccagccct ggtttatctc
     5941 cgtcaaaggc aaccacgaag cgatggcgtt ggatgcgttc gcgaccggcg atggcaatat
     6001 gtggcttgcc agcggtggtg actggttttt cgatttaaat gattcagagc aacaagaggc
     6061 aatagatctg ttgctgaaat tccatcacct tccacatatt attgaaatca ctaacgacac
     6121 cataaaatat gtcatcgcac atgcagatta tccggggaag gagtatcagt ttggcaaaga
     6181 aatagcggag agagaactac tctggcctgt tgatcgtgtg cagaaatcgc ttaatggcga
     6241 gttacaacaa ataaacggcg ctgattattt tatatttgga catatgatgt ttgataacat
     6301 tcagacgttc gctaaccaga tttatataga taccggatcg ccgaaaagcg ggcggctgtc
     6361 attttataaa ataaagtagt ctcatgcttc ttctgtgaag catgagtaac ccggcgttat
     6421 tgcaggtcat tattcatttt tcgctatcag caaagagaga tccagcttcg ccagcgcgcg
     6481 gctggcactc tccggcagac cgtcgtcagt aatgatctga tcaaactcgc ttaatggtaa
     6541 cgccagccat gtcgccacct gtccatattt cgtcgcatca cagaccaaaa ctcgctgacg
     6601 gctggcactg gcaatcgccc ttttcaccgt gactttatct tctgctggcg tagagatccc
     6661 ccgcatactc catgacgatg cagaaataaa agcctgatca atcatcaggc tacgcagcat
     6721 ggtcgcagcg gcttccccaa cacaggaacg gttttcccga cacactgcgc cgccagtgtg
     6781 aataattgtg caattactgt tgtcgagcaa gtagtccgca ataacgaagt cgttagtaac
     6841 cactgtgagt ggctccatgt gaatcagatg ctgtgcaatc gctaacgtgg tcgttcccgc
     6901 atccagatag atacaacgtc ccggctgaac aagacttgcc gccagcttgc caatagccgc
     6961 tttttgcgtc attgccagcg cagtttttac ctgatgagaa ggttcatgcg ccacccgtcc
     7021 cggagactgg acgcccccgg acaccagcac aacggctccc tgctgctcca gtttttgtaa
     7081 atcccgacga atggtcatat gtgacacatt cattctgtcc gttagttcag caatactaac
     7141 aatgcctttt tcagctacca tctcaaggat gatttggcga cgctctacgg gtatcaactt
     7201 ttgctccttc ctttgtcctg ctgacattct acgctatttg cctgcgaaac gtgcgcggcg
     7261 caactaacgc ttagttcaca taaaataaca cacaatgtta atttttgtga atcagctcac
     7321 catatcgtta tctttccgcg cttatattca caacataaaa caaaatatca cttaaattaa
     7381 caaggagagc agatgaaaac gggatcggag tttcatgtcg gtatcgttgg cttagggtca
     7441 atgggaatgg gagcagcact gtcatgtgtc cgcgcaggtc tttctacctg gggcgcagac
     7501 ctgaacagca atgcctgcgc tacgttgaaa gaggctggtg cttgcggggt ttctgataac
     7561 gccgcgacgt ttgccgaaaa actggacgct ctgctggtgc tggtggtcaa tgcgacccag
     7621 gttaaacagg tgctgtttgg tggaaaaggc gttgcacaac atctgaaacc cggtacggca
     7681 gtaatggttt cttccactat cgccagtgct gatgcgcaag aaattgctac cgctctggct
     7741 ggattcgatc tggaaatgct ggatgcgcca gtttctggtg gtgcagtaaa agccgctaac
     7801 ggtgaaatga ctgtcatggc ctccggtagc gatattgcct ttgaacgact ggcacccgtg
     7861 ctggaagccg ttgccggaaa agtttatcgc atcggttcag aaccgggact gggttcgacc
     7921 gtaaaaatta ttcaccagtt gttagcgggc gtacatattg ctgccggagc cgaagcgatg
     7981 gcacttgcag cccgtgcggg gatcccgctg gatgtgatgt atgacgtcgt gaccaatgcc
     8041 gccggaaatt catggatgtt cgaaaaccgg atgcgtcatg tggtggatgg cgattacacc
     8101 ccgcattcag ccgtcgatat ttttgttaag gatcttggtc tggttgccga taccgccaaa
     8161 gccctgcact tcccgctgcc attggcctca acagcattga atatgttcac cagcgccagt
     8221 aacgcgggtt acgggaaaga agacgatagc gcagttatca agattttctc tggcatcacc
     8281 ctaccgggag cgaaatcatg atcaagattg gcgttatcgc cgatgatttt accggcgcga
     8341 cggatatcgc cagttttctg gtggaaaacg gtctaccaac ggtacaaatt aacggtgttc
     8401 caacaggtaa aatgccggaa gcaatcgacg cactggtgat cagcctgaaa acgcgttcct
     8461 gtccggtggt tgaagccaca cagcaatcgc tggcggctct gagctggttg caacagcaag
     8521 gttgcaaaca gatctatttc aaatactgct ctactttcga cagtactgcg aaaggtaata
     8581 tcggcccggt taccgatgca ttaatggatg ctctcgacac gccgtttacg gtcttctctc
     8641 cggccctgcc ggtcaacgga cgtacggttt atcaggggta tttgttcgta atgaatcaac
     8701 tgctggccga atccggaatg cgccatcacc cggtaaatcc catgaccgac agctatcttc
     8761 cccgtctggt tgaagcgcaa tccacagggc gctgcggcgt cgtttcggca catgttttcg
     8821 aacaaggtgt ggatgccgtt cgtcaggagc tggctcgctt acagcaagag ggctatcgct
     8881 acgcggtgct tgatgcgctg accgaacacc atctggaaat tcagggggaa gccttgcgcg
     8941 atgccccact ggtaacgggc ggttctggtc tggcgattgg cctggcccgg cagtgggcgc
     9001 aagaaaacgg taaccaggct cgcgaagcag ggcgtccgct cgctgggcgc ggcgtagtgc
     9061 tctccggttc atgctctcaa atgaccaacc gccaggtggc acattaccgt caaattgcac
     9121 cagcccgtga ggttgatgtg gcacgctgcc tctcaaccga aactctggcc gcttatgcac
     9181 acgagctggc agagtgggtt ctgggccagg aaagtgtact tgctccactg gtttttgcca
     9241 ccgccagcac tgacgcattg gcagcaattc aacagcaata cggtgcacaa aaagccagtc
     9301 aggcagtaga aacactgttt tctcaactag cggcgcggtt agcagcggaa ggcgtgacac
     9361 gctttattgt cgcaggcggt gagacctccg gcgtagtcac acagagcctg ggaataaaag
     9421 ggtttcatat tggcccaacc atttcccccg gcgtgccgtg ggtaaacgca ctggataagc
     9481 ctgtctcact cgcccttaaa tctggcaact tcggtgatga agcctttttt tcacgagccc
     9541 aaagagagtt tttatcatga gcgatttcgc aaaagtagag cagtctttgc gagaggagat
     9601 gacgcggatt gccagttcat tctttcagcg cggctatgca accggttcgg ctggcaatct
     9661 gtcgctgctt ttacctgacg ggaatttact ggcgacaccg acaggttcat gcctgggcaa
     9721 tctcgatccg cagcggcttt ccaaagtcgc cgcggatggc gaatggttaa gtggtgacaa
     9781 accctcgaaa gaggtgctct ttcatctggc gctgtatcgc aacaatccgc gctgtaaagc
     9841 ggtggtgcat ttgcacagca catggtcgac ggcgctttcc tgcctgcaag ggctggacag
     9901 cagcaacgtt attcgtccgt tcacaccata cgtggtgatg cggatgggaa atatcccgct
     9961 ggtgccttat taccgaccgg gcgataaacg catcgcacag gatctggcgg aactggcagc
    10021 agacaatcag gcttttttac tggcaaatca tggcccagtg gtttgtggtg aaagcctgca
    10081 agaagccgcc aacaatatgg aagagctgga ggaaacggca aagctgattt ttattctcgg
    10141 tgaccgcccg atccgttatc tgaccgcagg tgaaattgcg gaattaagga gttaatgcaa
    10201 tgcctcgttt tgcagctaat ttatccatga tgttcaccga agtgcctttt attgaacgct
    10261 tcgccgcagc gcgaaaagcc gggttcgatg ctgtggagtt tctgtttccc tatgactact
    10321 ccccccagca aatccagcag caactggcgc aaaaccacct gacgctggcg ctgttcaata
    10381 ccgctcccgg agatattaat gcgggagaat ggggattatc cgcccttccc ggacgtgagc
    10441 acgaagcacg tgcagacatc gacctggcac tggaatatgc attggcgctc aattgtgaac
    10501 aagtccatgt gatggcaggc gtcgtgcccg ctggcgaaga tgctgaacgg taccgggcag
    10561 tatttattga taatctccgc tacgctgccg accactttgc gccacacggc aagcgaatat
    10621 tggttgaagc actcagcccc ggcgttaagc ctcattatct cttctccagc cagtatcagg
    10681 cactggctat tgttgaagag gttgcgcgag ataacgtttt tattcaacta gatactttcc
    10741 atgcgcaaaa agtagatggc aacctgacgc atttaatccg cgactacgcc gggaaatatg
    10801 cgcacgtaca aattgccgga ctacctgatc gacatgaacc ggacgatgga gaaatcaact
    10861 atccgtggct gttccgcctg ttcgatgaag tgggatatca gggctggatc ggttgtgaat
    10921 acaaacctcg tggcctcacc gaagaagggc ttggctggtt tgacgcctgg cgctaattcg
    10981 ctcaatccct aattacaacg tacccataca tccccccatc tacggatggg gtaggttaac
    11041 tataactttc agacagggtt tcccatgtcc acaattacat tgttatgcat tgcgttagct
    11101 ggcgtaatca tgctgttgct gctggtcatc aaggcaaagg tacaaccatt cgttgctctg
    11161 ctcctcgtca gcctgttagt cgcacttgcg gcgggtatac cggcgggcga agtgggtaaa
    11221 gtgatgatcg ccgggatggg cggtgtgctt ggttccgtca ctattattat tggtctgggc
    11281 gctatgctgg ggcgtatgat cgaacactct ggtggtgcag agtcactggc taattatttc
    11341 agtcgcaagt taggcgacaa acgaactatc gctgcgctga ctctggcagc gttcttcctc
    11401 ggtattcccg tcttctttga tgtcggcttt attattcttg cgccaatcat ttacggtttt
    11461 gccaaggttg ccaaaatatc accactcaaa tttggcctgc ctgtcgctgg gatcatgctc
    11521 actgttcacg tggcggtacc gccgcatcca ggccctgtcg ccgcagcggg gttactccac
    11581 gcagacatcg gctggctaac catcatcggt attgcgattt ctattcccgt agggattgtt
    11641 ggctactttg cagcgaaaat aatcaataag cgccaatatg cgatgtcggt cgaggtactg
    11701 gaacagatgc aactggctcc ggccagtgag gaaggcgcga caaaattaag cgataaaata
    11761 aatccaccgg gcgtcgcgct ggtcacctcg ctaattgtta ttcctatcgc gattatcatg
    11821 gcgggtacgg tttccgcaac actgatgccg ccttcgcatc ccctgcttgg tacgctacag
    11881 ctgatcggct caccaatggt agctctaatg attgcgctgg tgctggcatt ctggttattg
    11941 gctttacgtc gcggctggag cttacaacac accagcgaca ttatgggctc agcccttccc
    12001 actgcggcgg tagtgatttt ggttactggt gctggagggg tatttggcaa agtgctggtg
    12061 gaatcgggcg tcggcaaagc ccttgccaac atgctacaaa tgattgacct gcctctgtta
    12121 ccagccgcat ttattatttc actggcgctg cgtgcatcgc agggatcagc aaccgtagca
    12181 atcctgacaa ccggcgggtt actctcagaa gcggtgatgg gattgaatcc gattcagtgc
    12241 gtattggtga cgctggcagc ctgctttggt gggcttggtg cgtcacatat taatgactca
    12301 gggttctgga ttgtgaccaa atatctgggg ttgtcggtag cagacggtct gaaaacctgg
    12361 acagtgttaa cgaccattct cggttttacc ggattcttaa ttacctggtg cgtatgggcg
    12421 gtaatttgac cttaataaaa ggtccgatgg gcatcggacc ttttatttag cgcttacctt
    12481 ccgccagcag cggcgggatg ctcggcacca tcggcgcgtc atcaatatct ttctgcgtca
    12541 tgcggaacgc ttcgggataa tgttcgcggc tggtgcggcg tagcggttcg gtatcgcgcc
    12601 aggtatagag gcaatgctgg cactggtaga ccatccagac atcttttacc ggcgatttcg
    12661 ccatcacttc aatctgttca tcggcacaac gtggacaaat catcttgttc tccttattga
    12721 cgtgcggcca gcatagcggt cagtttttca gcccaggctt tggtttccgg taaatccacc
    12781 accggctggc tgtagtgacc acggttgtcc ggagcgacag gcgtggtggc gtcgataatc
    12841 agcttgtcgg tgataccggc cgggcttgag ccggggtcga gttccagcac ggacatattc
    12901 ggcaactgca ccaaatcccc tgccgggttc actttcgagg agagcgccca catcacctgc
    12961 ggcaggttga acggatcaac gtcttcatcg accataatca ccatcttcac gtagcccaga
    13021 ccgtgcggcg tggtcatcgc acgcaggccc accgcgcggg caaagccgcc gtagcgtttt
    13081 ttggtggaga taatcgccag aaggccgtgg gtgtacatgg cgttcaccgc ctgcacttcc
    13141 gggaactcgg ctttcagttg ctgatacagc ggcacgcagg tggctggccc catcaggtag
    13201 tcgatttccg tccacggcat cccgagatag agcgattcaa aaatgggttt gctgcgataa
    13261 gagactttat caatgcgcac gacggtcata ttacgcccgc cggagtagtg cccggtaaac
    13321 tcaccgaacg gcccttcgat ttcgcgttta cggctttcga tgaccccttc gaggatcact
    13381 tccgagcccc acggcacatc aaaaccggtc agcggggcgg tggcgatcgg gtacgggctt
    13441 tcgcgcagcg cgcctgccat ttcatactca gactgatcgt atttcagcgg cgtggccccc
    13501 atcagggtga tgatcggatc gttaccgagc gtgattgcaa tcggcagatc ttcaccgcgc
    13561 tcttccgctt tatgcagatg cagggcgata tcgtgcatcg gcaccggttg cagaccgagc
    13621 ttacgcttac ccttcacttc catacggtag atgccgacgt tctgcttgcc gaagttatcc
    13681 ggatcaagcg gatcgcggga aaccacgcac gctttgtcga gatagaaacc gccatcaccg
    13741 tcgtttaaac gaaacagtgg cagaatatcg aacaggttga tctcgtcgcc atcgacggtg
    13801 ttctgcgccc aggctggatt ggcgcggcgc tccggggcaa tggggaagtt atcccagcgg
    13861 cggataaact catcaatctg ttttttaacc ggggtgtttg gcggcaggcc gagggaaatc
    13921 gcgtggttct gccaggaacc gatggtgttc attgccacgc gggcatcgat aaagccgcga
    13981 atattatcaa accaaagcgc gggcgcgccg tcgccgatac gcccggtggc gttggctgcg
    14041 gctgccagat ccggttccgc gttcacctct tcgctgattt tcagtaactg gccgtggtca
    14101 tcaagcgcct gtaaaaagct gcgtaaatca tcaaatgcca ttattcattc tcctgagaaa
    14161 aattccggac ctgcggcaat ccttgccagc gcctggcgtg gggatgttcg aggccaaatt
    14221 gatccagcac gcgggctacc acgtggtgga caatgtcatc taccgtttcg ggatggttat
    14281 aaaaggcagg catcggcggc accatcgcca cgcccatgcg tgaaagtgcg agcatatttt
    14341 cgagatggat ggtgctaagc ggcatttcac gcggcaccag cactagtttg cggccttctt
    14401 tgagcacgac gtccgccgcg cgccccacca ggccatcggc gtaaccggca cggataccgg
    14461 cgagcgtttt catactgcac ggaataacga tcatgccgtc ggtacgaaag gaacctgagg
    14521 agatggtcgc cgcctgatcc gccgggttat ggctgaagtc tgcgagggca gcaacatcgc
    14581 gggcgctgta aggcgtttcc agttcaatgg tggttttcgc ccacgtcgac atcaccagat
    14641 gagtctcgac attcggcatc tcccgcagcg cttgcagtaa tgccacacca agaggcgcac
    14701 cggtagcccc tgtcatcccg acgatcagtt tcattgctta ctctctaaat tgttcgtata
    14761 cgaacgttat tattaaaata cgcctctgtt ttcatcgcgg caagcgctga aaagtaacaa
    14821 catcttcttc gtcactgaaa aaccgctatg atagcggcag atagtttgcc cggagtttac
    14881 atggcgttac gaaataaagc gttccatcag ttacgacagc tttttcagca gcatacagct
    14941 cgctggcagc acgagttacc tgacctgacc aaaccacagt acgcggtgat gcgcgccatt
    15001 gctgataagc ctggtatcga acaggtggca ctgatagaag cagcagtcag caccaaagca
    15061 acgctggcag aaatgttggc aagaatggaa aatcgtggct tagtcagacg tgaacatgac
    15121 gccgctgaca agcggcgacg ctttgtctgg ctgactgccg aaggcgaaaa gatacttgct
    15181 gcggcgatac cgataggtga cagtgtggat gaggaatttt tggggcgttt gagtggagaa
    15241 gagcaggagc tgtttatgca gctggtgcgc aagatgatga gcaaatagga tgttgattgc
    15301 cggatgggac gctgacgtgt cttatccagg cgacgaaaca taccaactcc ccatacaaaa
    15361 gaaaaaggcc agcctcataa gagactggcc tttctgacag atgcttactt attcgcggaa
    15421 cagcgcttcg atattcagcc cctgcgtttg caggatttcg cgcaaacggc gcaggccttc
    15481 aacctgaatc tggcgaacac gttcacgggt gaggccaatt tcacgaccta catcttccag
    15541 tgttgccgct tcgtacccca gcaaaccgaa tcgacgtgcc agtacttcac gctgtttggc
    15601 gttcagctcg aacagccatt tgacgatgct ctgcttcata tcgtcatctt gcgtggtatc
    15661 ttccggaccg ttctcttttt catcggccag gatgtccagc aacgcttttt cggaatcacc
    15721 acccagcggg gtgtctaccg aggtaatgcg ctcgttaaga cgaagcatac ggctgacgtc
    15781 atcaactggc ttatccagtt gctctgcgat ctcttccgca cttggttcgt ggtccagttt
    15841 atgggacaac tcgcgtgcgg ttcgcaggta aacgttcagc tcctttacga tgtgaatcgg
    15901 caaacgaata gtacgggttt ggttcataat cgcccgttcg atcgtctggc gaatccacca
    15961 ggttgcgtat gttgagaagc ggaaaccacg ttccgggtca aacttctcta ccgcgcggat
    16021 cagccccagg ttgccctctt cgatcaggtc cagcaacgcc agaccacgat tgccataacg
    16081 gcgggcaatt tttaccacca gacgcaagtt actctcgatc atccggcggc gagaggcgac
    16141 atctccacgc agtgcgcgac gcgcaaaata aacttcttct tcggccgtta acagtggtga
    16201 ataaccaatc tcaccaaggt aaagctgagt cgcgtccagc acacgctgtg tggctccctg
    16261 cgataacagt tcctcttcgg ccaaatcgtt atcactgggt tcctcttcta ctaaggcctt
    16321 ttcgtcaaaa acctcaactc cgttctcatc aaattccgca tcttcattta aatcatgaac
    16381 tttcagcgta ttctgactca taaggtggct cctacccgtg atcccttgac ggaacattca
    16441 agcaaaagcc tggttccgcc gatttatcgc tgcggcaaat aacgcagcgg gtttacggat
    16501 ttccccttgt aacgaatttc aaaatgcaag cgtgttgaac tggttccggt gctacccatg
    16561 gttgctattt tttgccccgc cttcacttct tgttgttccc ggaccagcat tgtgtcgtta
    16621 tgggcgtagg cactcaggta atcatcatta tgtttgatga taatcagatt accgtagccg
    16681 cgcagcgcgt taccggcata aacaacgcgg ccatctgcgg tcgcgataat tgcctgtcct
    16741 ttgctgcctg cgatatcaat ccccttgttg cccccctcag aagcgccaaa ggtttcgatc
    16801 actttgccct cagtcggcca gcgccaggtg gagataggcg tactggttga tgtactgctg
    16861 acagtcggct cggttgtgct tgctgttggt accgttacag gcgctgtgac cgtggtcgca
    16921 gttggcttgt tgttcggcaa cattttgtta gcactctgtt cacccgaaga ctcagaatac
    16981 gtaattgtcg gttgcgacgc aacagcaacg gtggaatttt gtgcaggctt gatcacaact
    17041 ccttgctctg ctgcgtcggc ctgggtaatg gcatttccgc cagtgattgg cgtaccggaa
    17101 gcattaccca cctgtaaggt ctgaccgacg ttcagcgcat atggtgcctg aatattgttg
    17161 cgctgagcaa ggtcacggaa atcgttgcca gtaatccagg cgatatagaa aagtgtgtcg
    17221 ccttttttca cggtataggt actgccgcta taactgcctt tcggaatgtt cccatactga
    17281 cggttgtaga cgatgcgtcc gttttccatc tgtaccggct gctgagctac tggctgtact
    17341 ggctggattt gcggttgttg agtagcctga atttgtggct gctgtaccgg ctgaatttgc
    17401 ggttgctgcg ctgtagacgt cgtccccatt ttcggcggcg gcgtaatcaa cataccagaa
    17461 ttggtatttg caggcgcatt gccattaacg gagctgaccg gtgccggtgg atttgaagtg
    17521 tcagaacagc ctgccagcca tagcgaaacc agtgacaaag ccgcaatgcg gcgaacggtg
    17581 aattttgggc ttcccgcgct catttatccc ccaggaaaaa ttggttaata accagtgaca
    17641 taattaccgt gcaaggcacc atactgaaca ctggaaaaga tgttcacgat acgctgacct
    17701 gcggcaaaat aaccaggaaa aatccaggta tttcctcacg ttttaagcca gctcaccctt
    17761 cactaaaggg acaaagcgca cggcctccac ggtatcgata ataaattcgc ctccccgacg
    17821 acgcacccgt ttcaaatact ggtgctcctc ccctacgggt aagacgagaa ttccgccttc
    17881 gtccagctgc gtcattagcg cagttggaat ttccggcggt gccgccgtaa caatgatagc
    17941 gtcaaacggc gcacgtgcct gccaaccttg ccatccatcg ccatgacggg ttgaaacatt
    18001 atgtaaatca agatttttca ggcggcgacg cgcctgccac tgcaagcctt taatccgttc
    18061 aaccgagcaa acatgctgga caagatgcgc caggattgcc gtttgatatc ccgaaccggt
    18121 gccaatttcc agcacccgcg actgcggcgt cagttcgagt aattcggtca ttcgcgccac
    18181 catatacggc tgcgaaattg tctgcccctg acctatcggc aaggcgatat tgtcccaggc
    18241 tttttgttca aacgcttcat caacgaattt ttcacgcggc acggcggcaa gtgcattcag
    18301 caccagctca tcctgaatac cttgcgcacg taattgatcc agaagtgctt gtacgcgtct
    18361 gcttaccatt gcgtgccaac tcccacgctg tttaaccagt ctgaaaccac atcttgcgcg
    18421 ctatgcgcag ttaaatccac atgcagcggc gtgatggaaa catagccctc atctaccgca
    18481 gcaaaatcgg tccccggacc ggcatcacat ttaccgcccg gcgggccaat ccagtacagc
    18541 gtattgccgc gcggatcttg ctgcgggatc acctgatctg ccggatgtcg tgtaccgcag
    18601 cgcgtcacgc gaataccttt gatttgatcc aatggtaaat ccggaacgtt aatattaaga
    18661 atacgcccgg tgcgcagcgg ctctttacac agtgcgcgca aaattgagca ggttaccgcc
    18721 gcggcagtgt cgtaatgttt atgcccgtca agcgagacgg caagcgccgg aaaacctaaa
    18781 tgacggcctt ccatcgcggc cgctaccgta ccggaataaa taacatcatc ccccagattc
    18841 ggcccggcgt taattccgga cacaacaatg tccgggcgcg ggcgcatcag agcatttacg
    18901 ccaagataga cgcaatcggt cggggttccc atttgcacag caatatcacc attttcaaag
    18961 gtaaacgtgc gcagggagga ttccagtgtc agagaattag aagcgccgct gcggttacga
    19021 tcgggggcga ccacctgaac gtcagcaaac tcacgcaagg ctttcgccag cgtttgtata
    19081 ccgggtgcat gtaccccgtc atcattactc agcaatatgc gcataatcac ctgttgtgtt
    19141 gataagttcc ctgacaacgc tggttgcaaa acttcccgcc ggaagccaga aacggatttc
    19201 tacggtgacg tcatcccacc agttccagct taattgttgc ggatacagca gcatcgctct
    19261 gcgcgcggct tcaacttttt cgcgcaccag taaggtttgt aattcagttt cttcggcgac
    19321 agctgcttgt tcgaatgcca gcgcttcacg ctgagttccc cattcgccac tgcccggcaa
    19381 tgcggcggtt atcatcagct ctttatcgtt gacgcgacgc tgtaattccg ccagttcttc
    19441 ggtggttgcg acaaaccagc taccacgtcc ggctaattgt agcgcatcgc cgtcaacaac
    19501 ttgattaacg tctgcttttt tgaggcgctc agcaacaatc tgattaaaca acgcactgcg
    19561 ggctgccgac aaccaaaaac tacgtttatt gcgatcgcgc accggagtat tggtttgcgc
    19621 ccagcgcagc gcgccctgca agttgctacc gccaatccca aaacgttggg caccgaagta
    19681 gttcggtaca ccttttacgc aaatatcgat cagacgttgt tcaacgtcat cgcgattgct
    19741 cacttcgcgc agaaccaggg taaaggcgtt acctttcagc gcgcctaaac gcagcttgcg
    19801 cttgtgccgc gcatactcca tcacctggca gccttccagt tgaaaggcgc tcagatcggg
    19861 catttccttg cccggcacgc gagcacataa ccactgttcc gtaacagcat gtttgtcttt
    19921 ttgcccagcg aagctgactt cacgggcatg aattttcagg aatttcgcca gtgcatccgc
    19981 cacaaaacgg gtattgcagc cgtttttgag gattctaacc aaaatatgct caccttcacc
    20041 atcaggctca aagcccaaat cttccaccac cacaaagtct tccggattgg ctttcagcag
    20101 cccggtgcct tgcggtttac cgtggaggta agtgagatta tcaaactcaa tcattttgtt
    20161 gccttaatga gtagcgccac cgcttcacag gcaatccctt ccccacgtcc ggtaaatccc
    20221 agtttttccg tagtagtggc tttcacgtta acatcatcca tatggcagcc gaggtcttcg
    20281 gcaataaaca cgcgcatttg tgggatatgc ggcagcatct tcggcgcctg cgcgatgata
    20341 gtgacatcga cgttgccgag ggcataacct ttcgcctgaa tacgacgcca ggcttcgcgc
    20401 aacagctcgc ggctgtcggc acctttaaat gccggatcgg tatccgggaa cagcttgccg
    20461 atatctccca gcgccgccgc gccaagcaat gcatcggtca acgcatgcag cgccacgtcg
    20521 ccatcagaat gcgccagcag ccctttttcg taaggaatgc gcacgccacc aatgataatt
    20581 gggccttcac cgccaaaggc gtgtacgtca aaaccgtgtc caattcgcat tatgtattct
    20641 cctgatggat ggttcgggtg aggtaaaatt cggccagcgc caaatcctcc ggacgcgtta
    20701 ccttgatgtt atccgcgcgg ccttcaacaa gttgcggatg aaaaccgcaa tattccagcg
    20761 ccgaggcctc atcggtaatg gtagcgcctt catttaaagc gcgagtcaga cagtcatgta
    20821 acaactcacg agggaaaaat tgcggcgtca gtgcgtgcca taagccattg cgatcaacgg
    20881 tgtgagcaat ggcattttta cccgtctcgg cgcgtttcat cgtatcgcgc accggcgcgg
    20941 cgaggatccc tcccgtgcgg ctggtttcgc tcaacgccaa caatcgcgcg aggtcatcct
    21001 gatgcagaca aggacgagcg gcgtcatgca ccaataccca ctgcgcgtcg ccagcggctt
    21061 tcagacctgc cagcacggaa tcggcacgct cttcaccacc atcaacaacc gtgatttgtg
    21121 gatgattcgc cagaggaagt tgtgcaaagc ggctatcgcc aggacttatg gcaatgacga
    21181 catgtttcac ccggggatgc gccagcagcg catgcaccga gtgttcaaga atggtttgat
    21241 taccgattga gagatattgc ttaggacatt ccgtttgcat tcgacggcca aatccggccg
    21301 ccggaaccac ggcgcaaaca tccaaatgag tggttgccat gttaattccc gggctgattt
    21361 atcgattgtt ttgccccgca gactgtgcgc gcttcgacgc gtcaggcacc agacgataaa
    21421 aagtttcgcc cggcctggtc atgctgagtt cattacgcgc acgctcttcg agcgcctcct
    21481 ggccgccatt gagatcgtca atttcggcaa aaagctgatc gtttcgcgct ttaagtttcg
    21541 cgtttgtagc ttgctgtgcc gccacatcat tattgacgcg agtatagtca tgtataccgt
    21601 tcttaccgaa ccacagcgaa tactgtagcc agaccagaat agccagcaac agcagcgtta
    21661 gtttacccat cctgccccct gaaaaacggc atcatcatcc catgcatccg aagacgactc
    21721 tacatcctct gttggggata ccgcgacaac gcgggcaaat gtaccacatt tgtccattgt
    21781 tacgtatacc cagggcgtgc agaacataat ctcattatta gttacggttt gaattatgaa
    21841 cagaggagac aagaaagtac aaattagccc agtagccaca taaacagtgc gccaaacata
    21901 atgcctactg tcaccagggt gaaaacaata ctgtagcgta gctttccgtc catcaatgaa
    21961 tgcagcgcaa tccccaccac taccgcgaca ggcatcagtg ccagaaagaa aggccaggtg
    22021 tagataaaga agaacagcgt gttagagcca taaatcaaca tcggcatcgc cagcgcaaat
    22081 aaccatgaga taaaaccgac cacggcacca ggcagtgacc atgtggtttc ttcatcctca
    22141 gtaaggctgt cgttatttgt tagtgtaatg ttatggctat tacgcatatt tgatcctgtt
    22201 actttgacga accgggcatg gaaacccggt ggtgtctcag gatctgataa tatcgttctg
    22261 tctcaacaga tctaataatt gctgtaccaa atttgttact aattgttcac cattgagatg
    22321 aatttctgcc gattcaggcg cttcgtaaac ggaatctatt cccgtaaagt tgcgcagttc
    22381 accggcacgc gctttcttat ataagccttt tggatcgcgg gcttcgcaaa tcgccagcgg
    22441 cgtatcgaca aacacttcga taaagcgccc ttctcctacg cgttcacgaa ccatctggcg
    22501 ttcggcgcgg tgtggcgaga tgaatgcggt cagcaccacc agtccggctt caaccatcaa
    22561 attcgccact tcaccgacgc gacggatatt ctctttacga tcggcatcgc taaaaccgag
    22621 atcgctgcat aatccgtggc gaacattgtc gccatccagc agatacgtac tgacgccaag
    22681 tttatgtaac gcctcctcca gcgcccccgc gacggttgat ttaccggaac cagagaggcc
    22741 ggtaaaccac agcacaacac cacgatgacc gtggtgctgt tcgcgttgtt gcggagttac
    22801 tgggtggcta tgccagacga cgttttcgtc atgcagagcc attatttatc tcccagtaaa
    22861 tcgcgcgcac cccaatgcgg gaaatggcgg cgaaccaggg cattcaattc cagttcgaat
    22921 gcactgaatt cagatggcgc agcagttgcc tggctaactg gttcgtgcac cataccggca
    22981 cctacggtca cattactcag gcgatcgata aaaatcagcc cacctgtaac cgggttttgc
    23041 tgataacgat ctaacaccag tggctcgtca aaggtgagat ctaccaggcc gatgccgttc
    23101 agcggcaggt tttcaacttc gcgttgggta aggttattga tatcaacctg ataacgaatg
    23161 ccatcaacac gagcacgcgt cttcttaccg gcaattttga tgtcataact ctggcccggg
    23221 gaaagcggct gttccgccat ccataccaca tccaccgacg cgctctgcac agccggtaac
    23281 gcttcgtctg ccgccagcag cagatcgcca cggctgatgt cgatctcatc cgtcagcacc
    23341 agggtgatcg cttctccgga aaaggcttct tcgcgatcgc catcaaaagt cacgatccgc
    23401 gcgacgtttg attccacacc agagggcagc actttgacac gttgcccgac ttccacgcga
    23461 ccggatgcca gcgttccggc gtagccacga aaatcgagat tcggacggtt aacgtactgc
    23521 accgggaagc gcattggctg agcatccacc actcgctgga tctccacggt ttccagcact
    23581 tcgagcagtg tcggaccgct gtaccacggc atactttcac tttgcgaagc cacgttgtcg
    23641 ccttccagtg cggagagcgg cacaaagcgg atatccagat tacccggcag ctgcccggca
    23701 aaagtcagat aatcttcacg aatacgggtg aacgtctctt cactgtaatc caccagatcc
    23761 attttgttga tcgccacgac cagatgtttg atccccaaca gtgtggagat aaaactgtga
    23821 cgacgggtct gatcgagcac gcctttacgg gcatcgatca gtaagatcgc cagttcacat
    23881 gtcgatgcgc cagtcgccat attgcgggtg tactgctcgt gccctggggt gtcggcgata
    23941 ataaatttac gcttctcggt agagaaatag cggtaggcca catcaatagt gatgccctgt
    24001 tcgcgctcag cttgcaggcc gtccaccagc agtgccagat ccagcttttc gccctgggtg
    24061 ccgtgacgct tactgtcgtt atgcagcgat gagagctgat cttcgtagat ttggcgggta
    24121 tcgtgcagca ggcgaccaat cagggtactt ttgccgtcat cgacgctacc acaggtcaga
    24181 aaacgcagca ggcttttatg ttgttgcgca atcatccagg cttcgacgcc gccttcattg
    24241 gcgatttgtt gtgcaagtgc ggtgttcatc ttaaaaatac ccctgacgtt ttttcagctc
    24301 catagacccc gcctggtcgc ggtcaatcac gcggccctga cgttcactgg tggtggaaac
    24361 cagcatctct tcgatgatct ccggcagtgt ttgtgcattt gactccaccg caccggtcag
    24421 cggccagcag cccagcgtac ggaaacgcac catccgtttt ttgatcactt cgcccggttg
    24481 caggtcgata cggttgtcat caatcatcat caacataccg tcgcgttcca gaaccggacg
    24541 ttccgcagcg agatacagcg gaacaatgtc gatattttcc agccagatgt attgccagat
    24601 atcctgctcg gtccagttag agagcgggaa gacgcgaatg ctttcgcctt tgttaatttg
    24661 cccgttgtag ttgtgccaca gctccgggcg ctgatttttc ggatcccagc gatggaagcg
    24721 gtcacggaaa gagtagattc gctctttagc gcgggatttc tcttcgtcac ggcgcgcacc
    24781 accgaaggcg gcatcaaaac cgtatttatt cagcgcctgt ttcaggcctt cagttttcat
    24841 aatatcggta tgtttcgcgc tgccatgaac gaatggatta atccccatcg ccacgccttc
    24901 cgggttttta tgcaccagca gctcgcagcc gtaggcttta gccgtacgat cgcggaactc
    24961 atacatctcg cggaatttcc agccggtatc gacatgcagc aacgggaaag gcagcgtacc
    25021 tggataaaac gccttgcgcg ccagatgcag catgacgctg gaatctttac cgatagagta
    25081 gagcatcacc ggatttgaga attctgccgc cacctcgcga ataatgtgga tgctttccgc
    25141 ctccagttgc cgcaggtgag taagtcgttt ttgatccata accgttcctt tgcaataccg
    25201 ctattttctt gccatcagat gtttcgacta tagggagcgt aagagaacga atgaaattac
    25261 caattagaat gagtagttcc ttaacggaat aacgatttgg caaagctaat atcaaaaagt
    25321 gcttaaggca ccggatttcg agcgtttagg aagatttgaa attgttttag cgcagcggca
    25381 gtttcatact atggcggtaa aaaaatttgc atggtattta aggactcact atgttttccg
    25441 cattgcgcca ccgtaccgct gccctggcgc tcggcgtatg ctttattctc cccgtacacg
    25501 cctcgtcacc taaacctggc gattttgcta atacccaggc acgacatatt gctactttct
    25561 ttccgggacg catgaccgga actcctgcag aaatgttatc tgccgattat attcgccagc
    25621 agtttcagca aatgggttat cgcagtgata ttcggacatt taatagtcgg tatatttata
    25681 ccgcccgcga taatcgcaag agctggcata acgtgacggg aagtacggtg attgccgctc
    25741 atgaaggcaa agcgccgcag cagatcatca ttatggcgca tctggatact tacgccccgc
    25801 tgagcgatgc tgacgccgat gccaatctcg gcgggctaac gttacaagga atggatgata
    25861 acgccgcagg tttaggtgtc atgctggaac tggctgaacg cctgaaaaat acgcctaccg
    25921 agtatggtat tcgatttgtg gcgaccagcg gcgaagagga aggaaaatta ggcgctgaga
    25981 atttactcaa gcggatgagt gacaccgaaa agaaaaatac gctgctggtg attaatctcg
    26041 ataacttaat tgttggcgat aaattgtatt tcaacagcgg tgtaaaaacc cctgaagcag
    26101 taaggaaatt aacgcgcgac agggcgctgg caattgcgcg tagtcatgga attgccgcaa
    26161 cgaccaatcc gggtttgaat aaaaattatc cgaagggtac tggctgttgt aatgacgccg
    26221 aagttttcga caaagccggt attgccgtac tttcggtaga agcaacaaat tggaatcttg
    26281 ggaataagga tggttatcag caacgcgcaa aaacagctgc attccctgcg ggaaatagct
    26341 ggcatgacgt aagactggat aatcaacaac atattgataa ggcacttcca ggaagaatag
    26401 aacgtcgctg ccgtgatgtt atgcggataa tgctaccgct ggtgaaggag ctggcgaagg
    26461 cgtcttgatg ggtatgaaaa tgggagttgg gagttctacc gcaggggcgg ggaattctaa
    26521 gtgatatcca tcatcgcatc cagtgcgtcc ggtttatccc cgctgacgcg gggaacacac
    26581 atgaatgtcg gttcagaccg tgtttttacc cggtttatcc ccgctggcgc ggggaacaca
    26641 gcgcgggcta atgtctgctc tgaatcatca ccggtttatc cccgctggcg cggggaacac
    26701 catgctgccg tcgtttacgc cgttcttaat ttcggtttat ccccgctggc gcggggaaca
    26761 caacaggccg tatgaggatc actggcacgg cggcggttta tccccgctgg cgcggggaac
    26821 actttctagg aaacgacgtt atttgctcat cccacggttt atccccgctg gcgcggggaa
    26881 cacaccccga aattaatggc gacggggcgt ttacgcggtt tatccccgct ggcgcgggga
    26941 acacatcaaa ctgctgcaac ggccagcgca gccacgcggt ttatccccgc tggcgcgggg
    27001 aacactgcct cggcggcggc tctgcgcaac cgcgaaacgg tttatccccg ctggcgcggg
    27061 gaacactcgt tttgttagcc agccgtcgcg caccagttcg gtttatcccc gctggcgcgg
    27121 ggaacacgta ttgaatcgtg tgtaaattcg cggcggcccc ggtttatccc cgctggcgcg
    27181 gggaacacac taagcataca taactgtttt taaacaaatt tattccgcat caacaatcta
    27241 ccaactaaat taaaacattt ccttattttt aaagaacgga taacttattg attatcaaca
    27301 ggaagaaaag aaaccaaacg caacccatcc aaatccaccg gaatacgtct attttcaccc
    27361 caggtctgaa attcaaaacc tgactcggta ttggtcgccc aggccatcac tacatttccg
    27421 caacctgcca gttgagagat ttgctgccag atcatctccc gaatacgttt ggacgtatct
    27481 ccgacataaa caccggcacg cacttcaagt agccagatcg cgagccgtcc acgtaagcgc
    27541 ggagggacat tttctgtaac aacgacgacc atactcatcc gccgcgtccc cggtgaccgc
    27601 tatcgcccag cgtttcaggt tcagggatgg caggcggtaa catatccggc gcgggttgtg
    27661 gtggttcaat ttcacctgcg gcaagcactt cctcgattaa cggaattaat ttgcccgtta
    27721 acttactgct acggaaaata tcacgacagg ccaacctgac ttctttatct ggttctgcgg
    27781 gttgcctcgc tgctatttca aatgcctttg gcacaaccga atcaaattta atgatatcgg
    27841 ctatgtcata aacaaatgaa agcggtttgc cactatggat aaatccaata gcgggcgcat
    27901 atcccgcggc taatactgcc gcttcagaaa taccgtacag acatgatgtg gcagcactga
    27961 tgcagcgatt cacaacatcg cctttttccc agtctttagg atcgtatttg cgaccattcc
    28021 atttcacacc atattgtttc gccagtaatg catacgtctg gcgaacgcgg gatccctcaa
    28081 ttccacgtag ctgctccact gaacggcgag ctggcggtgg ctcacgaaaa cgtaattcat
    28141 acattttgcg caccaccttc agacgtagat cttccgttaa agccagcttt gcctggtaga
    28201 gtaacttatc agcacgcgcc ccaccgggtt gtccggaaga gtagacgcga acacccgctt
    28261 caccgaccca gaccagtagc gtccccaccg tggcggccag atgcaccgcc gcgtgggaaa
    28321 ctctcgttcc cggttcgagc ataatgcagg cgaccgatcc caccggaatg tgcgtgcgga
    28381 tcccggtttt gtcgatcagc acgaaagcgc cgtccagtac gtcgatttga ccgtactgga
    28441 ggaagatcat ggaagtgcga tcttttaatg ggatcgggct cagcggtaca aacgtcacgc
    28501 ctctgctccg ggtttgatca gcatcagacc acaaccgaac gcccggcttt tgccataccc
    28561 ctgactgaga cgttgcagaa ataacccagg atcggtgacc gtaagcatcc ccgtataatc
    28621 cacgctactg aactggatca gttgccggga gttttcccgc cgcagttgct gttgtcggta
    28681 ggcatcaacc gaagtatcca gcagtgtaaa accgcttctc tccccctgcg ctgccagcca
    28741 gtccagcgca gcctgttgtt gatgcaacca gacatcactt ccttccacct gccccctcac
    28801 ctgccgtttc gcctccatca gcaagtcgtg gcgtttgccc gctttacaaa ttgttggatt
    28861 cgcccgcagg ttgaaacaaa gggattgtcc ggtacgcaac tctggcgcaa atggtcggca
    28921 ttcgatggtg aaaatatcac tttccgccgg gcgttcctgc gacaggacaa aaaagcgaaa
    28981 cgccccctgg agttcttccc gacgataaag aaattgcctt tctttgccgc caggaaaaag
    29041 atcccacagc cactgatgca ttacatattc accgcgatcc accagatgca gcaactgcgc
    29101 tggggaaagc tggccggtat gtaaggttat tcttgagagg tacatggttc ctccttgctg
    29161 agccacggcc cctgattgat ggtgcgttcc ccaaacagcc actgctgacg atttaaagga
    29221 acatcccggc gacgtaatat tttgctcgcg accaggccgt cgtgttcccc ttcccaccag
    29281 cattcatcct gaagtttcgg gagtgagact ttcagttcgc ggaaactatc ctgatactgt
    29341 tggtatgcgt tacgtaagac atcagacgcg ttgccttcga gcagtaacgg cgcaagtggt
    29401 aacgccagcg gatgactttt tcgccccaga taaagcggaa aaaccggatg acgtaaaccg
    29461 tcctgcaact gttcaaggct gtaaggcgca tcgggggttg ttgccaccgc caccatccac
    29521 caggcatcgg tgtagtagtc gcgccgggag ataatcgcgc tcagaagatc aggggcgctc
    29581 aactcttcgc gacggctgaa ataacgcgct ttacgcacct cttttggcat ctggaccgtg
    29641 tgataatccc gtgcccagcg cgggttacgg ctggcgcaaa ccaccagtga atagtggcgg
    29701 ttaaacgcgt ttaatcgttc ggtatcatca cgccgaatcc ctaccccggc agccagcagc
    29761 cccagcaatg ctgagcgcga aggcagttca tgggtatgac gcacttcgcc aggggcatcg
    29821 acgccccagg atgccattgg cccatgaagc tgaaaaatca aatattggct cattagccgc
    29881 cccttacgcg cagataaagt ccagcacgtc cttcatgctt ccctgtttgt tcatcacgtc
    29941 aaagcttgca cattcggtct tctgttcata gaccgtattc atattttcgc gaagcgttgt
    30001 aatacgctgc accgccacat ctaactggag ggtgccatta atgggttcat agaaagccgc
    30061 cgccagagag cgtggttgtt cagtgccttt ttctgccagc gcccaggagg cgtaggcacg
    30121 gctggcaaag ctgttctgtt tgccggttgg ggagacttta agtgcggctt ccgtaaaggc
    30181 gcgcaaggtc tgattagcta acgcttcgtc accgccgagg ttttcgacca gcagatcttt
    30241 atcgatgcag atataggtgt agaacagcgc agaaccgaat ccggtttcac caagatgccc
    30301 ggcaccggca tcttcagaag cctggcgcaa atcatcaacg gcggtgaaaa aatcatcttc
    30361 gacaatcgtt tcactgacac caaatgcatg cgcgacctgg caggcggctt caacattaaa
    30421 ttcgggttta ttcgccagca tacgaccaaa catagcgata tctaccgcca tgcgatcttt
    30481 acgtaacaaa gcgagatctt cctcttttgg cgcgcgcttt tcttcggcca gttgatgggc
    30541 cagcgctttt acggcgtcaa attctgccgg gctgatatgg actagctgtt cagtttcggc
    30601 gttagtgagc ggatcttttg gttttttgtc gtttttagct ttcccaagat aatccgcaat
    30661 ttttgccgcc cattcgatgg cttttttctc ttcgatgcct ttctcaatca ggatagttgc
    30721 cgcctcacgc gcaatacgcc cactgcgaat accaatatgg cccgccagtg cctgttcaaa
    30781 aagtgcagaa gtgcgccacg cacgtttcag actttgtgag gaaacgcgca gacgcgttgc
    30841 tccacccagt accacagttt ttggcgcacc ggtgtcatca cggttaaggt tggcagcagg
    30901 gtaagcggtt aacaaatgaa gctgaataaa tgtcgtcatg agatagtcct ttattaattt
    30961 tgttcgttat cggcgtcgcc agcctggtaa tattccagcg cccagcggat acggataaat
    31021 tctgttggcc gctgctggcg gcggtgatga ttgagcagat cgtcgctctc ctggcaccag
    31081 cggaaaatat cgtctgccaa agagataaga ttgacggaac catttaataa tttgaccgcg
    31141 cgacgtaact ggcgcagtaa ttcatccggc gttttgactg ccgacaggcg agtaaaacgc
    31201 ccctcagaca ttaccgccgc caactgcgcg gcaaaaggct gccgttcatc aatggctttt
    31261 acattggcgg caacggcggc aaccagcgcc agtgcggtaa aacgccactc ctgtgattca
    31321 attttccaca acgtgtgggt ctgcatcagc agagaacgaa acccttctga cagacaaaca
    31381 tcattaaccg ttgtgctacg ccgcagactg gcacgattgc cgcgtttttc ctgcaactct
    31441 tcgtgccatt tacgcagcgt ggctttgtgc tcctctttta caatactcat tcagcagcct
    31501 cctgcttttt ctccctggcg gctttagcac tttgcttctc cgccgatgtt gtaaaatatt
    31561 tcttgcgcgc ggtcatgacg cgtttcaaat caacgggctc ataaggatta gtgaatacac
    31621 gctcgtcaaa atcctgacgc gcgaataacc aaatttcctt ttgccatttg ccgagtaatt
    31681 catccgcatc ctgaccttct tcaatttggc gcactaacct caggaagcga tgctgagttt
    31741 tgttccagaa gtcgatatcc acaaaactga aatcacccct tacacctttt ggatcggaga
    31801 accatgcttc tttcaatgca ctccgtaaca gactcagaat ccgtgaagcc gtttgcgcag
    31861 ccagccgcag cttcggtatc tggccttctt ttttattgag cagtagcggg aaatggtgtt
    31921 cgtaccaaca gcgcgctttc atgttgtcaa aatcataacc aaatccccat aggcccactt
    31981 ttgcctgttt cagactgctg gcattaaaga gtttcaccac cagtgcagga agttccgtat
    32041 tgttttctga cttacccgtt tcgataaggc ctaaccagtc gcgccagatt aaaccgcccg
    32101 gttgtggttt gaccgagtaa aactcgccac cctctttaag tggtatacgg taaggcgtta
    32161 aaggatgctg ccacatggca taattcgcac cgtaattttt ggtcgtcatc aggctcagga
    32221 gcgcgtcgtt ctgctcgccg caaatatcgc agttgccgac tgtcgtggta ttaaaatcaa
    32281 tacgaatacg ccgcggcatt ccccagtacg cctggagttt attaacctga tcatcggtta
    32341 ccaccgcacc ggccagttcg ctggtgcgcg tcgggccaag ccaggggaaa accagaccgt
    32401 caaatttttt gggtagcggt aagtcggctt catcctgcgg catcacgttg agccacattt
    32461 tgcgccacaa gggagtttgt tgattgccct gatattcctg taattcaatc agagtagtca
    32521 tcggcccacc gccgcgtaag ccggtgcgat agcctttgcc gcctgacggc gcatttaact
    32581 gtagagagaa caaggctagt gcagaacact gtgggcacag gtgttccgtt acaccacgct
    32641 tgataaagtg gtctttatta aactttgttg tttgaacgcc gggaatctca ggcagtagcg
    32701 aagcgaccgg aactttatct cccgtgagcg cctcaaaatc ctgcataaat gaaggggaat
    32761 ctggcccaaa ctggaaagcg tgctctaatg gcagcaatgc ctcccgtagc ttttcagctt
    32821 ccagtccgtc ttcccagata tcatcccaac gacgatgatc ttttggcgcg aagctggttt
    32881 gtagtagccc cagcaaaaac tgccatgccg ccccctggag atctgcccgc ggcgcagcta
    32941 tatcgacaac attttcatcc gccagatgga ctggcgccag tttgcctgtt gttccgtctt
    33001 taaaacgaac gggcaaccac ggggttgtca gaagtgaaaa cgagttcata cgcatcctct
    33061 cgtaaaaaag accatccctg gccggttgtc catccttcca tctttttctg tgtatcaact
    33121 attcagagag ccctgtcgca ggcatctcaa ccaaccttgc cagtcaatcc ttccatagcc
    33181 gaatacccgc aactttcgtc atcggtgact aaaatcacgt ttgccatctc cggatcttgc
    33241 cgctgttcaa tgcaccactg cctgaacgca tccccttcca gtaaagaaaa ctcatcccga
    33301 tgttttttcc accagcttcg acgcactctg acaacgctca tttcccatgc gtgagcaccg
    33361 gtggcataag gcttcaccac accgtcaata caggtagcca gccacaggga aacagattcc
    33421 tcagccagac gtgtcgacag cttttccgga aggtaatcgt tgatattggc ggcatagcca
    33481 ggcttgaagt tcaggacaaa ctttttagcc attgcgcgat cgcagtaata tttgcccact
    33541 tgctcctgct cactgcgggc aaatccttcc ggcattgcca cgtcttcacc gtagactgat
    33601 tcaataagaa gccgggctgc gtgtggcatc tgaatagcgc cttgctcacg cagcacacgc
    33661 tgcgtcagcc agattcgccc atgatcgggg tagacatatg cactgttacg catggcgctg
    33721 ccaaaccatt cgtcacccgg agagtcgtcc cagacgggag ccagaatcag caattcagga
    33781 ggggaacgct cgtcttttcc gtcacgcttt aacagaccat taatatcgcg gatatgccgc
    33841 tgtaatcgcc ccgcccgctg gatcagtaaa tcaacagggg caaggtcgga aatcatttcg
    33901 tccaggtcgc agtcaacgct ctgctccagg acttgagtac aaatgaggac ttttccggca
    33961 cgctgtgaac agtcttcttt accaaagcgt gccagcgtct ccgtttcaat tcgctggcga
    34021 tcgctaaaag caaagcggct atgaaaaagt gaaaggctgg aagcaggaat gacgccgcgg
    34081 gcaagtaact gacgatgaac ctggatggcg tcatcgacag aatttcggat ccaggcgata
    34141 catttcccct gacttaccgc cgattcgata cgcgcaatac actcttgttc gctatgaagc
    34201 caacccacac tcacgctgcg ctcaacgtct ttgcgcgtcg ctacccggtg tgaatgcaca
    34261 tcggatttcg taacgtgcgt cagccagggg taatcatcct tttccagcaa cggagcttct
    34321 tgctggccct ctataccacg cgcaaaggcg gcgacgagtt tgtcgcgctg ctgttgagat
    34381 aacgtggcag aaagcaaaat gacgctgttt ccgccacgcg cctgccgctc gatcagccct
    34441 tcaagaatgc atgacatgta agcgtcacag gcatggatct catcagccag caggattttg
    34501 ttactcagtc ccagaagccg cagattatta tgtttgaacg gcatcacagc catcatcgcc
    34561 tgatccagcg tgccgacgcc aatttcagcc agtagcgcct tcttgttact gtcggcaaac
    34621 caggccgcac atccctgact gaacgtttgt tcatccggtt cttctgaccc gactaaatca
    34681 ccggaccaga gtgattcatt aaagcggtcc attaatgttc gggcactgtg tgccagcacc
    34741 aggctggggc gggactctgg cgaatagaaa gcaagccagg ttttgaccag tcgatcgtac
    34801 atggcattgg ccgttgccat tgttggcagg ccaaaaaaca aaccctgcgc tttccccgca
    34861 gccatcaacc tgtgcgccag gataagcgcc gcttctgttt tacctgcgcc ggttacatcc
    34921 tccagaataa ataactgtgg tcccggctgg ctgatatcca gatccagcac cttttgctgt
    34981 agcggtgtcg gattctcaat aaaaggaaac agcgtattaa ttccggtgaa aggcgcgctt
    35041 tctgcttttg gaggaaagac ggttaaggcg ttttgagcct gaactaaagt tttctgccag
    35101 taatcttcaa tatccattgc ctgtgcaacg cgtggaaaat atcgcgttga cgatcccgtc
    35161 cagtctgcaa gtacgacggt tgcagagata taccaggaaa gttgttttat aagttcaacg
    35221 ccctcgtcat catcccagaa tgtggggatc tctatgagcg gaaacagtac cttgattgca
    35281 aggagaaaat ctcgtgcggc acctttgtct tcaggcagaa aattatccag ttcatccata
    35341 cgctcagggg gtcgaccatg atgcccggta gtcatgggca tccacatctc catcacccgt
    35401 ttaagtttgc gcggagagag tgaagatgaa ggaagcaact cctgacattc acttaaataa
    35461 tggttccaca accagtagcc cagcgttgaa tgagagatct tttcgtaatt ctttcttgcg
    35521 ccttccggaa ccttgagttg tgggtgcagg taaagttgct gaaaagagcg ggcaaatttt
    35581 ccaatatcgt gccagcacag cagccaggcg aaaaattgtg ctgccagctc cgtgtcagcg
    35641 aaacctaatt gacgaaaata gccagccagt ccgaagcaat tgcttttaac cattaaatag
    35701 cccatcgcgg ctacatccag cgaatgccag caaagaaggt gatagtcttc gcccccgtct
    35761 ttctcgccgc gtcgggtttt tccccagaaa taaaagaaag tcacaatatt tttatccttc
    35821 agtaaaactt aaaggatatt cacgc
//
LOCUS       DAPHCV010000045        35805 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.045, whole genome shotgun sequence.
ACCESSION   DAPHCV010000045 DAPHCV010000000
VERSION     DAPHCV010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.045"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(110..3259)
                     /locus_tag="RH033_002175"
     CDS             complement(110..3259)
                     /locus_tag="RH033_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HDV2991355.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3282..4475)
                     /gene="acrA"
                     /locus_tag="RH033_002176"
     CDS             complement(3282..4475)
                     /gene="acrA"
                     /locus_tag="RH033_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HDV2991356.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     AAYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4617..5264
                     /gene="acrR"
                     /locus_tag="RH033_002177"
     CDS             4617..5264
                     /gene="acrR"
                     /locus_tag="RH033_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HDV2991357.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPSITE"
     gene            5392..8754
                     /gene="mscK"
                     /locus_tag="RH033_002178"
     CDS             5392..8754
                     /gene="mscK"
                     /locus_tag="RH033_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HDV2991358.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(8966..9127)
                     /gene="rsmS"
                     /locus_tag="RH033_002179"
     CDS             complement(8966..9127)
                     /gene="rsmS"
                     /locus_tag="RH033_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HDV2991359.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(9141..9668)
                     /gene="priC"
                     /locus_tag="RH033_002180"
     CDS             complement(9141..9668)
                     /gene="priC"
                     /locus_tag="RH033_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HDV2991360.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLSEMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9738..10115
                     /locus_tag="RH033_002181"
     CDS             9738..10115
                     /locus_tag="RH033_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HDV2991361.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10268..10819
                     /gene="apt"
                     /locus_tag="RH033_002182"
     CDS             10268..10819
                     /gene="apt"
                     /locus_tag="RH033_002182"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDV2991362.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10948..12879
                     /gene="dnaX"
                     /locus_tag="RH033_002183"
     CDS             10948..12879
                     /gene="dnaX"
                     /locus_tag="RH033_002183"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDV2991363.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEENGNTVCLHLRSSQRHLNNS
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    12212..12276
                     /gene="dnaX"
                     /locus_tag="RH033_002183"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12932..13261
                     /locus_tag="RH033_002184"
     CDS             12932..13261
                     /locus_tag="RH033_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDV2991364.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13261..13866
                     /gene="recR"
                     /locus_tag="RH033_002185"
     CDS             13261..13866
                     /gene="recR"
                     /locus_tag="RH033_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDV2991365.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEHEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13976..15850
                     /gene="htpG"
                     /locus_tag="RH033_002186"
     CDS             13976..15850
                     /gene="htpG"
                     /locus_tag="RH033_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDV2991366.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16031..16675
                     /gene="adk"
                     /locus_tag="RH033_002187"
     CDS             16031..16675
                     /gene="adk"
                     /locus_tag="RH033_002187"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDV2991367.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            16807..17769
                     /gene="hemH"
                     /locus_tag="RH033_002188"
     CDS             16807..17769
                     /gene="hemH"
                     /locus_tag="RH033_002188"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HDV2991368.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17766..18725)
                     /gene="aes"
                     /locus_tag="RH033_002189"
     CDS             complement(17766..18725)
                     /gene="aes"
                     /locus_tag="RH033_002189"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HDV2991369.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQHY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEACFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASTLWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            18877..20181
                     /gene="gsk"
                     /locus_tag="RH033_002190"
     CDS             18877..20181
                     /gene="gsk"
                     /locus_tag="RH033_002190"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HDV2991370.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20265..21941)
                     /locus_tag="RH033_002191"
     CDS             complement(20265..21941)
                     /locus_tag="RH033_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HDV2991371.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALDLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22179..23399)
                     /gene="fsr"
                     /locus_tag="RH033_002192"
     CDS             complement(22179..23399)
                     /gene="fsr"
                     /locus_tag="RH033_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HDV2991372.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            23617..25269
                     /gene="ushA"
                     /locus_tag="RH033_002193"
     CDS             23617..25269
                     /gene="ushA"
                     /locus_tag="RH033_002193"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HDV2991373.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHPQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25306..25785)
                     /gene="ybaK"
                     /locus_tag="RH033_002194"
     CDS             complement(25306..25785)
                     /gene="ybaK"
                     /locus_tag="RH033_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HDV2991374.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDTPAQEFATIYVSGGKRGLDIELAASDLAKILDAKFADIARRD"
     gene            complement(25989..26783)
                     /locus_tag="RH033_002195"
     CDS             complement(25989..26783)
                     /locus_tag="RH033_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HDV2991375.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDISLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACETLEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDNGLALLDDTLTHWHTNARLLQQMMSWWLNTPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            26916..27218
                     /locus_tag="RH033_002196"
     CDS             26916..27218
                     /locus_tag="RH033_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDV2991376.1"
                     /translation="MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIIN
                     AATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYRLIFKWVNGKAEELFLDPHNY"
     gene            27254..27595
                     /locus_tag="RH033_002197"
     CDS             27254..27595
                     /locus_tag="RH033_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDV2991377.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(27653..30157)
                     /gene="copA"
                     /locus_tag="RH033_002198"
     CDS             complement(27653..30157)
                     /gene="copA"
                     /locus_tag="RH033_002198"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HDV2991378.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSVTEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTNEGEK
                     NVPLADVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHAVLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            30420..31352
                     /gene="glsA"
                     /locus_tag="RH033_002199"
     CDS             30420..31352
                     /gene="glsA"
                     /locus_tag="RH033_002199"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDV2991379.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKVGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            31355..32647
                     /locus_tag="RH033_002200"
     CDS             31355..32647
                     /locus_tag="RH033_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV2991380.1"
                     /translation="MMNTEGNNDNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGALLLSLIVAMVMKRNKTV"
     gene            32772..33179
                     /gene="cueR"
                     /locus_tag="RH033_002201"
     CDS             32772..33179
                     /gene="cueR"
                     /locus_tag="RH033_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HDV2991381.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIEQHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(33180..33910)
                     /locus_tag="RH033_002202"
                     /pseudo
     CDS             complement(33180..33910)
                     /locus_tag="RH033_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898666.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(33982..34188)
                     /locus_tag="RH033_002203"
     CDS             complement(33982..34188)
                     /locus_tag="RH033_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2991382.1"
                     /translation="MKEQRKIPLTHIMIIGAFIFAFLQVVLLASLVHAVNVNNEIQEG
                     LFQSGRIMVESLQHILSVQTGING"
     gene            complement(34185..34607)
                     /locus_tag="RH033_002204"
     CDS             complement(34185..34607)
                     /locus_tag="RH033_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2991383.1"
                     /translation="MMNSSIKSFSLLAVILLAGCSSPASRIADCQAQGVSHDTCYLAE
                     QQRQTAILSASEAQAFKNAEAAQHAQAAKKAIYKGFGMTFRMSSKNFAYLNDSLCAID
                     EDNADATVYQSGLYNVIVYHHTGKVALMKEGQFVGYLK"
     gene            complement(34693..35709)
                     /locus_tag="RH033_002205"
     CDS             complement(34693..35709)
                     /locus_tag="RH033_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HDV2991384.1"
                     /translation="MKTVNVALLALIISATSSPVVLAGDTIEAAATELSAINSGMSQS
                     EIEQKITRFLERTDNSPAAYTYLTEHHYIPSETPDTTQTPTVQTDPDAGQKTVAATGD
                     AQTPARYQSMINARQSAVTDAQQTQITEQQAQIVATQKTLAATGDTQNTAHYQEMINA
                     RLAAQNEANQRTTTEQGQKMNALTTDVAAQQQKERAQYDKQMQSLAQKSVQAHEQIES
                     LRQDSAQTQQQLSNTQKRVADNSQQINTLNNHFSSLKNEVEDNRKEANAGTASAIAIA
                     SQPQVKTGDVMMVSAGAGTFNGESAVSVGASFNAGTHTVLKAGISADTQSDFGAGVGV
                     GYSF"
BASE COUNT         8696 a         9341 c         9385 g         8383 t
ORIGIN      
        1 atactggttt tcgtatgaga tcctgagttg gtggttcaat gactccttaa tgttcgtcgg
       61 ttatgcataa aaaaggccgc ttgcgcggcc ttagtgatta cacgttgtat caatgatgat
      121 cgacagtatg gctgtgctcg atatcttcat tcttgcggct aaagcggcgg cgaaccacca
      181 caaagaatac cggaacgaag aagattgcca gtaccgttgc ggtcaccatc ccgcccatta
      241 caccggtacc tactgcgttc tgcgcgccgg aaccagcacc agtactgata accagcggca
      301 taacgccgag gataaacgcc agcgaggtca tcaggatcgg acgtaaacgc atccgcaccg
      361 catcaagcgt cgcttcaatc agacctttac cttctttatc catcaagtct ttggcgaatt
      421 cgacgataag tatcgcgttc ttcgccgaca acccaatggt tgtgagcagg cctacctgga
      481 agtaaacgtc attggtcagg ccacggaagg tggcagccag caacgcaccg ataaccccca
      541 gcggaacgac cagcataacg gagaacggaa tcgaccagct ttcatacagc gccgccagac
      601 acaggaacac gacaatcaac gaaatcgcgt acagtgacgg tgcctggttg ccggagagac
      661 gttcctgata ggacatcccc gtccagtcat agccaacacc ggtaggtagt ttgctcgcca
      721 gttgttccat cagctccatt gcttcaccgg tacttttacc cggtgccgcc tggcctaaga
      781 tttccatgga tggcaggccg ttgtaacgtt ccagacgcgg cgaaccgtac tcccaacgag
      841 aagatgagaa cgccgagaat ggcaccatct gaccatcagc agcacgaaca taccagtcgc
      901 cgatatcatc cggcagcata cggtatttcg cttctgacat gacgtaaact ttcttcacac
      961 gaccgcggtc gataaagtcg ttcacatagc taccgcccca tgcagcgccc agagtggtgt
     1021 taatgtcgtt gatagaaaca cccagcgcct gcgctttttc ctggtcgata tcaatcttaa
     1081 actgcggggt atcttccaga ccgtttggac gtacgctggt caacatatca gggtgcttcg
     1141 ctgcttctgc aagcagctgg ttacgcgcct gagtcagttt ttcgtgacca aggccagcct
     1201 ggtcaatcag ctcaaagtca aagccggttg cggtacccag ttccacgatt gcgggcaggt
     1261 taaaggcgaa aaccatcgca tctttgattt gcgagaaagc acgtgttgca cgcatggtaa
     1321 tcgcttcaac tttgttttct tcgcccggac gatcggccca gtccttcaag gaaacgaacg
     1381 caataccggt attctgacca cgtcccgcaa agccgaagcc gttaacggcg aacaccgact
     1441 caacgttgtt cttttctttg gtcagatagt aatgcgttac ctcattgagc actttctgcg
     1501 tacgttcctg cgttgcacct gctggcagct gaaccatggt cataaatacg ccctggtctt
     1561 catctggcaa gaaggagctt ggcagacgca cgaacagata ggccatgccg accacgatga
     1621 tcagatacag caccaggtaa cgccctgtac tgcgcagaat accgcctacg ctgtcggtgt
     1681 agtggtgcgt gctcttttcg aacatgcggt taaaccagcc gaagaagcct tttttacctt
     1741 ccccgtgatc gcctttggca atcggtttca gcatggtggc acaaagagcc ggcgtcagga
     1801 tcaacgccac cagtactgac agcgccattg ctgaaacaat ggtaatagag aactgacgat
     1861 aaatagcacc ggtagaaccg ccaaagaagg ccatcggtac gaataccgcc gacagtacca
     1921 tcgcgatacc gaccagagcg ccctgaatct gccccatcga cttacgggta gcttcttttg
     1981 gcggcaaacc ttcttccgcc ataacacgct caacgttttc taccacaacg atggcgtcat
     2041 ccaccaatag gccgatggcg agcaccatcc cgaacattgt tagcgtgttt atcgagaagc
     2101 caaaggcggc aaggacggca aaggtcccca gcaataccac cggtacggca atggtcggaa
     2161 tcaacgtcgc gcggaagttc tgcaggaaca gatacataac caggaacacg aggatgatcg
     2221 cttcgaccag cgttttaacc acttcgtgaa tagagatttt cacgaacggt gtggtgtcat
     2281 acgggtaaac aattttcaga cccgacggga agaacggttc catcttcgcc agttcagcac
     2341 ggattgccgc agcggtatcc agcgcgttcg caccggtcgc cagcttgatc cccagaccgg
     2401 aagccggttg gccgttaaac tctgcgatga tgtcgtagtt ctcaccaccc agctcaattt
     2461 tcgccacatc acgcagcagc acgcgggaac catcctgatt cactttcagc aggattttgc
     2521 cgaactcttc agtagaggtc agacgcgtct gagcaataat agaggcgtta agctgttggc
     2581 ctttcaccgg cggcgtacca ccgagctgac ccgccgcaac ctgggcgttc tgcgctttga
     2641 tggcagtaat gacatcaacc ggtgttagct ggaatttgtt tagttcattc gggttcatcc
     2701 agatacgcat cgcgtactgt gaaccgaaca actgaacgtc acccacgccc gacgtacggc
     2761 tgatggcatc tttcatattc gccgccacgt agtcggagat atcctcctgc gtcatggtgc
     2821 catcggtgtt gataacgccg acaaccatca ggaagctgct ggatgatttc tcaacgctca
     2881 ccccttgctg ctgtacttct tgtggcagca acggcatcgc cagctgcagt ttgttctgca
     2941 cctgaacctg cgcgatatcc gcatcagtac cagactcaaa ggtcagggtg atctgcacgg
     3001 tacccgtgga gtcactgtta gaggacatgt acatcaggtt atcgataccg ttcatattct
     3061 gttcgataac ctgtgtcacc gtgtcctgca ctgttttcgc atcagcgcca gggtaggagg
     3121 cggagatcgt tactgccggc ggtgcaatcg taggatattg cgccaccggc agtttgagga
     3181 tcgccagccc ccctgccaac atgatgataa tggcgatcac ccacgcaaaa atcgggcgat
     3241 cgataaagaa attaggcatg tcttaacggc tcctgtttaa gttaagactt ggactgttca
     3301 ggctgagcac cgcttgcggc ttgctggtta ttatcagcgg taacttcttg tgcttttacc
     3361 tggacaccag gacgcacttt ctgcagccca cttattacta cgcgatcgcc tgctttcaga
     3421 ccttctgtca ccagccactt atcgccgata gcctggcttg caacgatcgg acgggtttcc
     3481 actttgtcat ccgcgccaac caccagtacg gtggcatcgc cacgcggcgt acgggttacg
     3541 ccctgttgcg ggactaaaat agcgtttgga ttaagccctt cttccagacg tgcacgtacg
     3601 aacatacccg gcagcagagt gtgatccggg ttcgggaaga tagcgcgtag ggtgatagac
     3661 ccggtggtct gatcaacggt aacgtcagag aattccagcg taccgtcctg cgggaactta
     3721 atgccgtcac tggtgatcag cgacactttg gctttgccgt tctcttgttt cagcgtgcca
     3781 tttgccagtt cctgtttcag gcgcaggaag tcgttgctgg actgggtcac atcaacgtag
     3841 atcggatcaa gttgctgcac ggttgccagc gcagtcgcct gaccgttctg taccaatgcg
     3901 ccttctgtca cgttcgactt accaatgcga ccgctaatcg gagaggtaac tttggtgtaa
     3961 gccagattga ttcgcgcagt ttcaacggca gctttcgccg cagttaccgc agcattcgcc
     4021 tgttgcgcat cagccagagc ctgatcgtac tcttgtttac tgatgtactg agtaccgagc
     4081 agtttctgat aacgattcac cgtcaattgc gcgatatttg ctgcagcctg ggctttcgcc
     4141 agatcacctt tcgcactgtc gtatgccgcc tgataggtcg caggatcaat ctgatagaga
     4201 gagacacctg cttcgatgtc gctaccttct ttgaaattac gcttcaggat aatcccgcta
     4261 acttgaggac gaacttctgc gatccggtag gcactggtgc gacccggaag ctcggttgtg
     4321 atctgcagag gttcagtttt gactgttact acgccaacgg cgggcatctg ctggccacct
     4381 tgttgggcct gtttgtcgtc acatcctgtt agggctaagc tgcctgagag catcagaacg
     4441 accgccagag gcgtaaaccc tctgtttttg ttcatatgta aacctcgagt gtccgatttc
     4501 aaattggtca atggtcaaaa gttaataaac ccattgctgc gtttatatta tcgtcgtgct
     4561 atggtacata cattcacaaa tgtatgtaaa tctaacgcct gtaaattcac gaacatatgg
     4621 cacgaaaaac caaacaagaa gcgcaagaaa cgcgccaaca catcctcgat gtggctctac
     4681 gtcttttctc acagcagggg gtatcatcca cctcgctggg cgagattgca aaagcagctg
     4741 gcgttacgcg cggtgcaatc tactggcatt ttaaagacaa gtcggatttg ttcagtgaga
     4801 tctgggaact gtcagaatcc aatattggtg aactcgagct tgagtatcag gcaaaattcc
     4861 ctggcgatcc actctcagta ttaagagaga tattaattca tgttcttgaa tccacggtga
     4921 cagaagaacg gcgtcgatta ttgatggaga ttatattcca caaatgcgaa tttgtcggag
     4981 aaatggctgt tgtgcaacag gcacaacgta atctctgtct ggaaagttat gaccgtatag
     5041 aacaaacgtt aaaacattgt attgaagcga aaatgttgcc tgcggattta atgacgcgtc
     5101 gggcagcaat tattatgcgc ggctatattt ccggcctgat ggaaaactgg ctctttgccc
     5161 cgcaatcttt tgatcttaaa aaagaagccc gcgattacgt cgccatctta ctggagatgt
     5221 atctcctgtg tcccacgctt cgtaaccctt ccattaccga ataaccctga atctgactcc
     5281 aggatttttc ctggacattt tcgtcgttgc tattctggtt cactgcgtcg tgatattctt
     5341 gcggtttgac tttttcaggt cgttcttcag gttcagaaac cttcattcat catgactatg
     5401 ttccagtatt acaaacgatc acggcatttt gttttttcag catttattgc ttttgttttt
     5461 gtcttgttat gccagaacac ggcgtttgcg cgggcgtcat cgaatggtga tctgccgaca
     5521 aaagcggacc tgcaggcaca acttgactca ctgaataaac aaaaagatct ttctgctcag
     5581 gacaaactgg tgcagcagga tctgacagat acattagcta ccctcgataa aatcgatcgc
     5641 gtaaaagaag agacagttca gctacggcaa aaggttgctg aagcgccgga aaaaatgcgc
     5701 caggcgaccg cggcgttaac tgcacttagc gatgtcgata atgacgaaga aacgcgcaaa
     5761 attctgagta cgctttcgtt gcgccagctg gaaactcgcg ttgcccaggc gctggacgat
     5821 ttgcaaaacg cacaaaacga tctggcgtct tataacagcc agctggtttc gttacagacg
     5881 cagcccgaac gcgtgcaaaa tgcgatgtat aacgcttcgc agcagctgca acaaattcgc
     5941 agtcgtctgg atgggactga tgtcggcgag actgccttac gtcccagcca gaaagtgtta
     6001 atgcaggctc agcaggcgtt gctgaatgcc gagattgacc agcagcgtaa aagcctggaa
     6061 gggaacacag tcttacagga taccttgcaa aagcaacggg attacgtgac ggcgaacagc
     6121 gcccgtctgg agcaccagtt gcagctgttg caagaagcag taaacagcaa gcgtctgact
     6181 ttaaccgaaa aaacggcgca ggaagccgtc tccccggatg aagccgcgcg tattcaggct
     6241 aatccgctgg tgaagcagga actggaaatt aaccagcagt taagtcagcg tctgattacc
     6301 gcgactgaaa acggtaatca gttgatgcag caaaacatta aagtcaaaaa ctggctggag
     6361 cgggcgctgc aatcggaacg caatattaaa gagcagattg ccgtcctgaa gggcagcttg
     6421 ctgttgtctc gtatccttta ccagcaacaa caaacgctgc cctcggcgga tgaactggaa
     6481 aacatgacca accgcatcgc ggatttgcgt ctcgaacagt ttgaagttaa ccagcagcgt
     6541 gatgcactct tccagagcga tgcgttcgtc aacaaactgg aagaaggtca caccaacgaa
     6601 gtcaacagcg aagttcacga tgcattattg caagtggttg atatgcgtcg cgaattgctg
     6661 gatcagctta acaaacagtt gggtaaccaa ttgatgatgg ccattaacct gcaaatcaac
     6721 cagcagcagt taatgagtgt gtcgaaaaac ctgaaatcca tcctgactca gcaaatcttt
     6781 tgggtgaaca gtaaccgtcc aatggactgg gactggatca aagcgttccc gcaaagcctg
     6841 aaagatgaat ttaagtcgat gaaaatcacg gtgaactggg aaaaagcctg gcctgccgtt
     6901 tttatcgctt tcctcgccgg tttgccgctg ctgttgattg ccgggctgat ccactggcgt
     6961 ctgggctggc tgaaagcgta tcaacaaaaa ctggcttccg ctgtgggttc cctgcgtaac
     7021 gacagccagc tcaacacacc aaaagcgatc cttatcgacc tgatccgtgc gctgccggtg
     7081 tgcctgatta ttctcgcggt tggcctgatt ctgttgacca tgcagctcaa catcagcgaa
     7141 ctgctatggt cgttcagtaa aaaactggcg attttctggc tggtgtttgg cctgtgctgg
     7201 aaggtactgg agaaaaacgg cgttgccgtg cgtcactttg gtatgccgga acagcagacc
     7261 agccactggc gtcggcaaat tgtccgcatc agtctcgcgt tgctgcctat ccatttctgg
     7321 tctgtggtgg cagaactttc cccgctgcat ctgatggatg atgtgctggg gcaggcgatg
     7381 attttcttca acttgctgct gattgccttc ctggtgtggc cgatgtgccg cgaaagctgg
     7441 cgtgataaag agtcgcacac catgcgactg gtcaccatta ccgtactgtc gattatcccg
     7501 attgcgttga tggtgctgac tgccacgggt tatttctaca ccacgctgcg cctctccggg
     7561 cgttggattg aaaccgttta tttggtgatc atctggaacc tgctgtacca gacggtattg
     7621 cgcggcttaa gcgtagcggc gcggcgtatc gcctggcgtc gtgcgctggc gcgtcggcag
     7681 aatctggtga aagagggcgc agaaggtgct gaaccgccgg aagaacccac cattgcactg
     7741 gagcaggtta accagcagac gctgcgtatt accatgttgc tgatgtttgc gctgttcggt
     7801 gtcatgttct gggcaatttg gtccgatttg atcaccgtgt tcagctatct ggacagcatc
     7861 acgctctggc attacaacgg cactgaagct ggcgctgcgg tggtgaaaaa cgtcaccatg
     7921 ggcagtctgt tgtttgcgat tatcgcctca atggtggcct gggcgttgat tcgcaacctg
     7981 cctggtttac tggaagtgct ggtgctctcg cgactgaata tgcgccaggg cgcgtcgtat
     8041 gccattacta ccatccttaa ctacatcatt attgccgttg gtgcgatgac ggtgttcgga
     8101 tcgctgggcg tctcgtggga taaactccag tggctggcgg cagcattatc cgtaggtctt
     8161 ggttttggtt tacaagagat cttcggtaac ttcgtttccg gtttgatcat tctgttcgaa
     8221 cgtccggtgc gcataggcga cacggtaacc attggtagct tctcggggac ggtaagtaag
     8281 atccgtattc gtgcgacaac gattaccgat ttcgatcgca aagaagtgat tatcccgaac
     8341 aaagcgtttg ttaccgagcg tctgatcaac tggtcgttga ctgacacgac tacgcgtctg
     8401 gtgatccgtc tcggcgtggc ctatggctcc gatctggaaa aagtgcgtaa agtgttactg
     8461 aaggcggcga ctgagcaccc aagggtgatg cacgaaccaa tgccggaagt cttctttacg
     8521 gcatttggtg ccagcacgtt ggatcatgag ctgcgtctgt atgtgcgtga actgcgtgac
     8581 cgtagccgta ctgtcgatga gctgaaccgt actattgatc agttgtgccg tgaaaacgac
     8641 atcaacattg cctttaacca gcttgaagtg catctgcaca acgagaaggg cgatgaagtg
     8701 acggaagtga aacgcgacta caaaggcgac gatccgacgc cagcggtagg gtaataacca
     8761 aagggggaga catttagttg ccccgagatt actgacaaag tgcgcgttgt tcatgccgga
     8821 tgcggcgtga ccgccttatc cggcctacga aaccgcaaga attcaatata ttgcaggagc
     8881 ggtgtaggcc tgataagcgt agcgcatcag gcagttttgc gtttgcccgc aatcttaggg
     8941 gacatttagc gaccccattt atttctcact tttccgcctc atcatcgcgc gttaatttct
     9001 tttcataatc gcgttttaca atatccagcg cgcgcagtac ggtgcgggct gggatctgat
     9061 tttcttccag cagcacaatc aaatcgacgg ccagtttgac atcgtcaggg gcattttcca
     9121 gtgacatact ctctccattg ctagcgggtt aaacgcgcta acctgttttc gattttttcc
     9181 agcgcatggc ggcagcgtgc caggcgcgct tcataggctt ccacttcacg atgcagcgtt
     9241 tgctgttcca cgagatcggt cacccgcgcc aggcgggctc gacgttcagc aaccatctca
     9301 cttagccgcc gctcaaaatc ctgatgctga atacgtttac gctgccagcg ggcaattttc
     9361 ggtggcgcac tgtcccactc gcgtagcgac caggctgtgg cttcacgcgc tatagcctcc
     9421 agttgcgccg ccagatgttc cgccagccag gcgacttgcg gcagctgttg ctgctcaacc
     9481 gcatgacgaa gcgcagccag attatcgccc gcctcatcga gacaagcctg cagtgttgtc
     9541 gcacgagtct gaaaaagatg cctgtcaaaa cgggcgctta gcgtggcaaa ttgcgctacc
     9601 ggggcacaac gctgacgcag cgtagcgagc tgtccttcca gtttttccag cagcagggcg
     9661 gttttcacga taagacctca atgaaaatga taattgttat gctaaagtag caactctgta
     9721 agctgacaac actcaatatg caacgaatca ttttaatcat cattggctgg ctggcggtag
     9781 tgctgggtac gctgggcgtg gtattaccgg tattaccgac gacgccgttt atcctgctgg
     9841 cggcctggtg ctttgcccgt tcttccccgc gctttcacgc ctggttgctg taccgctcat
     9901 ggtttggcag ctatctacgt ttctggcaga aacatcatgc gatgccgcgc ggcgtcaaac
     9961 cgcgggcgat tttgcttatt ttgctcacgt ttgccatttc tctgtggttc gtccagatgc
    10021 catgggtgcg catcatgttg ctggtaattc tcgcctgttt gcttttttat atgtggcgaa
    10081 ttccagtgat tgatgaaaag caagaaaagc actgaagcac aacaatcgca gttgcaatta
    10141 ttgcgtacag ccagtacatt ctggcgtttt cgagcacagg cgcaggcggt caaaggttaa
    10201 acaactgtta cttttgatac gtttaaaacg cgccgtgagt accaccgtaa caagcaggca
    10261 tacacttatg accgcgactg cacagcagct tgagtatctc aaaaatagca tcaaaagcat
    10321 tcaggactac ccaaaacccg gcattctttt ccgcgatgtc accagcttac tggaagaccc
    10381 gaaagcttac gctctcagca tcgacttgct ggttgagcgt tacaaaaatg cgggcattac
    10441 caaagtggtc ggcaccgaag cacgtggctt cttgtttggc gctccggtag ctctgggtct
    10501 gggcgttggc tttgtaccgg tccgtaaacc gggcaaactg ccgcgtgaaa ccatcagtga
    10561 aacttacgac ctggaatacg gcaccgatca gctggaaatc cacgttgatg ccatcaaacc
    10621 gggcgacaaa gttctggtgg tggacgattt gctggcaacc ggcggcacta tcgaagcgac
    10681 cgttaaactg atccgtcgtc tgggtggtga agtagctgac gctgcgttca ttatcaacct
    10741 gttcgatctt ggcggcgaac agcgtctcga aaaacagggc attaccagct acagccttgt
    10801 cccgttcccg ggccattaat tatcgtcagt cttgcgctgc tcacgctacg ggcagcacaa
    10861 gatgtgcatt cagcctcgcc gttctgacgg ggctgtgtta gcattacccc ttcgtgaatc
    10921 caccttccag cgtttcagag cctgccaatg agttatcagg tcttagcccg aaaatggcgc
    10981 ccacaaacct ttgctgacgt cgtcggccag gaacatgtgc tgaccgcact ggcgaacggc
    11041 ttgtcgttag ggcgtattca tcatgcttat cttttttccg gcacccgtgg cgtcggaaaa
    11101 acctctatcg cccgactgct ggcgaagggg ctaaactgcg aaaccggcat taccgcgacg
    11161 ccgtgcggcg tgtgcgataa ctgtcgtgaa atcgagcagg ggcgctttgt cgatctgatt
    11221 gaaatcgacg ccgcctcgcg cactaaagtt gaagacactc gcgacctgct ggataacgtc
    11281 cagtacgctc cggcgcgtgg tcgtttcaaa gtttatctca tcgacgaagt gcatatgctg
    11341 tcgcgtcaca gctttaacgc actgttaaaa acccttgaag agccgccaga gcacgttaag
    11401 tttttgctgg cgacgaccga tccgcagaaa ttgccggtga cgattttgtc acgctgtctg
    11461 caatttcatc tcaaggcgct ggatgtcgag caaattcgcc atcagcttga gcacatcctc
    11521 aacgaagaac atatcgctca cgagccgcgg gcgctgcaat tgttggcacg cgccgctgaa
    11581 ggcagcctgc gagatgcctt aagtctgacc gaccaggcga ttgccagcgg tgacggccag
    11641 gtttcaaccc aggcggtcag tgcgatgctg ggtacgcttg acgacgatca ggcgttatcg
    11701 ctggttgaag cgatggtcga ggccaacggc gagcgcgtaa tggcgctgat taatgaagct
    11761 gctgcccgtg gtatcgagtg ggaagcgttg ctggtggaaa tgctcggtct gttgcatcgt
    11821 attgcgatgg tacaactttc gcctgctgct cttggcaacg acatggccgc cattgagctg
    11881 cggatgcgtg aactggcgcg caccataccg ccgacggata ttcagcttta ctatcagacg
    11941 ctgttgattg gtcgcaaaga attaccgtat gcgccagacc gccgtatggg tgttgagatg
    12001 acgctgctgc gcgcgctggc gttccatccg cgtatgccgc tgcctgagcc agaagtgcca
    12061 cgccagtcct ttgcgcccgt tgcgccaacg gcagtaatga cgccaacaca ggtgccgccg
    12121 caaccgcaat cagcgccgca gcaggcaccg actgtaccgc tcccggaaac caccagccag
    12181 gtgctggcgg cgcgccagca gttgcagcgc gtgcagggag caaccaaagc aaaaaagagt
    12241 gaaccggcag ccgctacccg cgcgcggccg gtgaataacg ctgcgctgga aagactggct
    12301 tcggtcaccg atcgcgttca ggcgcgtccg gtgccatcgg cgctggaaaa agcgccagcc
    12361 aaaaaagaag cgtatcgctg gaaggctacc actccagtga tgcagcaaaa agaagtggtc
    12421 gccacgccga aggcgctgaa aaaagcgctg gaacatgaaa aaacgccgga actggcggcg
    12481 aagctggcgg cagaagccat tgagcgcgac ccgtgggcgg ctcaggtgag tcaactttcg
    12541 ctaccaaaac tggtcgaaca ggtggcgtta aatgcctgga aagaggagaa cggcaacacg
    12601 gtgtgtctgc atttgcgctc ctctcagcgt catctgaata acagtggcgc acagcaaaaa
    12661 ctggctgaag cgttgagcac gttaaaaggt tcaacggttg aactgactat cgttgaagat
    12721 gataatcccg cggtgcgtac gccgctggaa tggcgtcagg cgatatacga agaaaaactt
    12781 gcgcaggcgc gcgagtccat tattgcggat aataatattc agaccctgcg tcggttcttc
    12841 gatgcggagc tggatgaaga aagtatccgc cccatttgat cgtaagcaca gcttacgttc
    12901 gtcatcctta acgtgattga gagagaaacc tatgtttggt aaaggcggtc tgggtaacct
    12961 gatgaagcaa gcccagcaga tgcaagaaaa aatgcagaaa atgcaggaag agatcgcgca
    13021 gctggaagtg accggcgaat ctggcgcagg tctggtaaaa gtgaccatca acggtgcaca
    13081 caactgccgt cgcgtagaga tcgacccgag cctgctggaa gacgacaaag agatgctgga
    13141 agacctggtg gctgcagcat tcaacgacgc ggcacgtcgt atcgaagaaa cgcagaaaga
    13201 aaaaatggcc tctgtatcct ccggaatgca gctgccgcct ggctttaaga tgccgttctg
    13261 atgcaaacca gcccgctgtt aacacagctt atggaagcac tgcgctgtct gccgggcgtt
    13321 ggcccgaagt cggcgcagcg tatggcgttc acgctgcttc agcgcgatcg tagcggcggg
    13381 atgcgtctgg cgcaggcgct cacccgggcg atgtcggaaa tcggccactg cgccgattgc
    13441 cgcaccttca ccgaacacga agtctgtaac atctgttcga atccgcgtcg tcaggaaaac
    13501 ggtcaaatct gcgtggtgga gagtccggcg gacatctacg ccattgagca gacggggcag
    13561 ttttcaggcc gttattttgt gttgatggga cacctgtcac cgctggacgg catcggtccg
    13621 gatgatatcg ggcttgatcg tctggaacag cgtctggcag aggaaaaaat cactgaagtg
    13681 atcctcgcca ccaaccccac ggttgaaggt gaagctaccg ctaactacat tgccgagctt
    13741 tgcgcgcaat atgacgtgga agccagccga atcgctcatg gcgtaccggt tggcggcgaa
    13801 ctggaaatgg tcgatggcac cacgctgtca cactcccttg ccgggcgtca taagattcgt
    13861 ttttaagcaa acgagagcag gatcacctgc tctcgcttga aattattccc ccttgtcccc
    13921 atctctccct catcctgttt ttaaccttaa aatggcatta ttgaggtaga cctacatgaa
    13981 aggacaagaa actcgtggtt ttcagtcaga agtgaagcag cttctgcacc tgatgatcca
    14041 ttctctctat tccaataaag aaatcttcct gcgtgagctt atctctaacg cctccgatgc
    14101 ggcggacaag ctgcgtttcc gcgcgctctc taacccggat ctgtacgaag gtgatggcga
    14161 actgcgcgtt cgtgtctctt tcgataaaga caagcgcacg ctgaccatct ccgataacgg
    14221 cgtggggatg acccgcgacg aagtgattga ccatctgggg actatcgcta aatccggcac
    14281 caaatcattc ctcgaatccc tgggttctga ccaggcgaaa gacagccagc tgatcggtca
    14341 gtttggtgtt ggtttctact ctgcgtttat cgtggccgac aaagtgaccg tgcgtactcg
    14401 cgcggcaggc gaaaaaccag aaaacggtgt tttctgggaa tcggctggtg aaggtgaata
    14461 caccgttgcc gacatcacca aagaagatcg tggtactgaa atcaccctgc atctgcgtga
    14521 aggcgaagac gagttcctcg atgactggcg cgtgcgttcc atcatcagca aatactccga
    14581 ccatatcgcg ctgccggtag agatcgaaaa acgcgaagag aaagatggcg aaaccgttat
    14641 ctcctgggag aaaatcaaca aagcgcaggc gctgtggacc cgtaacaagt cggaaatcac
    14701 cgatgaagag tacaaagagt tctacaaaca catcgcccac gactttaacg atccgctgac
    14761 ctggagccac aaccgtgttg aaggtaagca ggagtacacc agcctgctgt acattccgtc
    14821 ccaggctccg tgggatatgt ggaaccgcga tcataaacat ggcctgaaac tgtacgtcca
    14881 gcgtgtgttc atcatggacg acgcagaaca gttcatgccg aactatctgc gcttcgtgcg
    14941 tggtctgatt gactccagcg atctgccgct gaacgtttcc cgtgaaatcc tccaggacag
    15001 cacggtaacg cgtaacctgc gcaatgcgct gaccaagcgt gtgctgcaaa tgctggaaaa
    15061 actggcgaaa gacgacgcgg aaaaatacca gaccttctgg caacagtttg gcctggtact
    15121 gaaagaaggt ccggcggaag atttcgctaa ccaggaagcg atcgccaaac tgctgcgttt
    15181 tgcttctacc cataccgatt cttctgcgca gaccgtgtct ctggaagact acgtttcccg
    15241 catgaaagaa gggcaggaga aaatctacta cattaccgca gacagctatg cggcagcgaa
    15301 gagcagcccg cacctggaac tgctgcgtaa gaaaggcatc gaagttctgc tgctttccga
    15361 ccgcatcgat gagtggatga tgaactatct gactgagttc gacggtaaac cgttccagtc
    15421 tgtgtctaaa gttgacgagt cgctggaaaa actggctgac gaagttgatg agagcgcgaa
    15481 agaagcggag aaagccctga ctccgttcat cgaccgtgtg aaagccctgc tcggcgagcg
    15541 cgtgaaagat gtccgtctga ctcaccgtct gaccgatacg ccagcgatcg tttcgaccga
    15601 cgcggacgaa atgagcactc agatggcgaa actgttcgct gcggcgggcc agaaagtgcc
    15661 agaagtgaaa tacatcttcg aactgaaccc ggatcacgta ctggtgaaac gtgcggcgga
    15721 tactgaagat gaagcgaagt tcagcgagtg ggtagaactg ctgctggatc aggcgctgct
    15781 ggcagaacgc ggcacgctgg aagatccgaa cctgtttatt cgtcgtatga accagctgct
    15841 ggtttcttga tgccatgccg gatgaccttc gtgtcatccg gcatttttct tttcttcatc
    15901 tgcactttcc gcaaattatc ttgccattaa ccgtttcagc ctcaggtgcc tttcttgagg
    15961 caatcgcctg ttggtggtat cgtttatcgc tttttcaaaa aattcgacac attttaaggg
    16021 gattttcgca atgcgtatca ttctgcttgg cgctccgggc gcggggaaag ggactcaggc
    16081 tcagttcatc atggagaaat atggtattcc gcaaatctcc actggcgata tgctgcgtgc
    16141 tgcggtcaaa tctggctccg agctgggtaa acaagcaaaa gacattatgg atgctggcaa
    16201 actggtcacc gacgaactgg tgatcgcgct ggttaaagag cgcattgctc aggaagactg
    16261 ccgcaacggt ttcctgttgg acggcttccc gcgtaccatt ccgcaggcag acgcgatgaa
    16321 agaagcgggc atcaatgttg attacgttct ggaattcgac gtaccggacg aactgatcgt
    16381 tgatcgtatc gtcggccgcc gcgttcatgc gccgtctggt cgtgtttatc acgttaaatt
    16441 caatccgccg aaagtagaag gcaaagacga cgttaccggt gaagaactga ctacccgtaa
    16501 agacgatcag gaagagaccg tacgtaaacg tctggttgaa taccatcaga tgactgcacc
    16561 gctgatcggc tactactcta aagaagcgga agcgggtaac accaaatacg cgaaagttga
    16621 cggcaccaag ccggttgctg aagttcgcgc tgatctggaa aaaatcctcg gctaatcaag
    16681 ccgcagcaga tctgctttcg ggcaggtctg ctcatcctcc tcaggcaata cctctcacag
    16741 caattagttc ttcttcttca cttttccgct acaattatca acaagttgaa tcgataagag
    16801 gcggtaatgc gtcagactaa aaccggtatc ctgctggcaa acctggggac gcccgatgcc
    16861 cccacacctg aagcggtaaa acgctatctg aaacaatttt taagcgacag acgtgtggtt
    16921 gatacctcac ggttgttatg gtggccattg ctacgcggcg tgattttgcc gctgcgctcg
    16981 ccgcgtgtgg cgaagctgta tgcctctgtc tggatggaag gtggctcgcc gctgatggtt
    17041 tacagccgcc agcaacagca ggcgctggca caacgtttac cggagacgcc cgtagcgctg
    17101 ggaatgagct acggctcgcc atcactggaa agcgccgtag atgaactact ggcagagcat
    17161 gtagatcata ttgtggtgct gccgctttat ccgcaattct cctgttcaac ggtcggtgcg
    17221 gtatgggatg aactggcacg cattctggcg cgcaaacgta gcattccggg gatatcgttt
    17281 atacgtgatt acgccgataa ccacgattac attaatgcac tggcgaacag cgtacgcgct
    17341 tcttttgcca aacatggcga accggatctg ctgctgctct cttatcatgg cattccccag
    17401 cgttatgcag atgaaggcga tgattacccg caacgttgtc gcacaacgac tcgcgaactg
    17461 gcttccgcac tggggatggc accggaaaaa gtgatgatga cctttcagtc gcgctttggg
    17521 cgggaaccct ggctgatgcc ttataccgac gaaacgctga aaatgctcgg agaaaaaggt
    17581 gtaggtcata ttcaggtgat gtgcccgggc tttgctgcgg attgtctgga gacgctggaa
    17641 gagattgccg agcaaaaccg tgaggtcttc ctcggtgcgg gcgggaaaaa atacgaatat
    17701 attccggcgc ttaatgccac gccggaacat attgaaatga tggctaatct tgttgccgcg
    17761 tatcgctaaa gctgagcggt aaagaattga gcgccgtcgc gaagagcctc gtcggcggtt
    17821 ttcatcatcc gtgaataatg caaaaaggcg tgcagcgtgc ctgggtagag tttgaactca
    17881 cagggctgct gatgcgccgc taacgtctgg taaagcagac ggctgtcatc cagcagcgga
    17941 tcgaactccg ccccggcaat aaaacagggc ggaacttcgc gagtgagatc attattaaac
    18001 aggcagtaat acggcgactc gcggtccgcg tcgttactta aatacgcctc ttcatacatc
    18061 tgtaaatcct gttgcgttaa gccatcccag acaccgccca acagacgacg agtcacggaa
    18121 tcccgtaatc cgtaaagccc ataccacagc aaaacgcccg caactttacc gcaatcgatc
    18181 tgtttatcac gcaaccacaa cgtactggcg agcgccagca tggcacctgc ggaatcaccg
    18241 gcaaagccaa tgcgggacat attgatttga taatcttccg cctgctggtg gaaataacaa
    18301 caggcagcca caatttcctc tatcgcttgc ggaaaacacg cttcaggtga aagggtgtaa
    18361 tcaatgccaa tcaccgtaca ttggctgtag cttgccagca ggcgcatgat gcgatcgtgg
    18421 gtatcaagat tgccgagaat aaaaccgccg ccatgcaaat aaaatagcgt cgccgggcta
    18481 tctggctgcg gacaaaagag acgtgtttcc acctgcccat attttgttgg aaccatgtaa
    18541 gctctggttg ccatttctgg agcgcccgca ttccagaatc ggcgctcaag cgtgtaatgc
    18601 tgtcgttgct cagcaatcgt tcccgttgcg ggccagggcg gtaaatccgg ctgaagagta
    18661 ttcacaacgg tcttcatttc agcagaaata aggtccagaa cgggtaattt gttttccggc
    18721 ttcatgcaaa aactcctttc aaattacgtc attgtaagga accactgcca tgaaaatgcg
    18781 atcccgcctg ctgatattga aactggctgc gtctcgcgcg ctcccgtcag attgtgttaa
    18841 cattcgccgc tcagttaacc acccgtaaaa acaaccatga aatttcccgg taaacgtaaa
    18901 tccaaacatt acttccccgt aaatgcacgc gatccgctgc ttcagcaatt ccagccagaa
    18961 aacgaaacca gcgctgcctg ggtagtgggt atcgatcaaa cgctggtcga tattgaagcg
    19021 aaagtggatg atgaattcat tgagcgttat ggattaagcg ccgggcattc actggtgatt
    19081 gaggatgacg ttgccgaagc gctttatcag gaactaaaac agaaaaacct gattacccat
    19141 cagtttgcgg gtggcaccat tggtaacacc atgcacaact actcggtgct cgcggacgac
    19201 cgttcggtgc tgctgggcgt catgtgcagc aatattgaaa ttggcagcta tgcctatcgt
    19261 tacctgtgta acacctccag ccgtaccgat cttaactatc tacaaggcgt ggatggcccg
    19321 attggtcgtt gctttacgct gattggcgag tccggggaac gtacctttgc tatcagccct
    19381 ggccacatga accagctgcg ggctgaaagt attccggaag atgtgattgc cggagcctcg
    19441 gcactggttc tcacctctta tctggtgcgt tgcaagccgg gtgaacccat gccggaagca
    19501 accatgaaag ctattgagta cgcgaagaaa tataacgtac cggtggtgct gacgctgggc
    19561 accaagtttg tcattgccga gaatccgcag tggtggcagc aattcctcaa agaccacgtc
    19621 tctatccttg cgatgaacga agatgaagcc gaagcgttga ccggagaaag cgatccgttg
    19681 ttggcatctg acaaggcgct ggactgggtt gatctggtgc tgtgtaccgc agggccaatc
    19741 ggcttgtata tggcgggctt taccgaagac gaagcgaaac gtaaaaccca gcatccgctg
    19801 ctgccgggtg ctatagcgga atttaaccag tatgagttta gccgcgccat gcgccacaag
    19861 gattgccaga atccgctgcg tgtctattcg cacattgcgc cgtacatggg cgggccggaa
    19921 aaaatcatga acactaatgg agcaggggat ggcgcactgg cagcgttgct gcatgacatt
    19981 accgccaaca gctaccaccg tagcaacgta ccaaactcca gcaaacataa attcacctgg
    20041 ttaacttatt catcgttagc gcaggtgtgt aaatatgcta accgtgtgag ctatcaggta
    20101 ctgaaccagc attcacctcg tttaacacgc ggcttgccgg agcgtgaaga cagcctggaa
    20161 gagtcttact gggatcgtta agttatcgac cagaccggac gacgatggca tccgtaggcc
    20221 ggataagatg cgtcagcatc gcatccggca atggaatcaa tgttttaccc cgtcaccacc
    20281 tcacccgccg gtggcgtttc cagcagttcc agcatagtac gggcaatttc acgctcaccc
    20341 atcactacct gatttgcacc acgttcggtg atatacgcca cttcatcgtc ataatgggcg
    20401 cgggcgataa tctcaatatc cggatttttc gcgcgggcag atgccacaat ctcgcccgct
    20461 tcataaccgt tgggaatcgt caggatcagc cattttgcac attccagatg cgccagttgc
    20521 ataatttctt cgttcgccgc attgcccaat actgcgcgga ccccgcgctc tcgcagttca
    20581 tcaacacggg ttcgtgacgt ctcaatcacc accagcggaa tatcagaggc gagcaatttc
    20641 tcccccagca ggctgcctac acgaccgtaa cctaccagta gcgcatggtt gcaaatatcc
    20701 actgggatct gcttctcttc ttcgatagcc tcttccagcg tctgctcttc cagcgtttcg
    20761 gtcttcgcca gatatttctc cagcagcgca aacaggaccg ggttgagcat aatcgacaaa
    20821 atcgcccctg ccagtaccag gttctgcccg gcttgcggca acagatccag cgccatgccc
    20881 agtcccgcca ggataaacgc gaactcacca atttgcgcca ggctggcggc gatagttaat
    20941 gccgtacgtt gggagtgacc aaacagtcgc accaggaaaa acgcggctaa cgacttacca
    21001 aacagaataa tcgccagcgt cgccagcact gccagcggtt gctgaatcag aattaacgga
    21061 tcaaacaaca tcccgacgga gacaaaaaac agcaccgcaa acgcgtcgcg caatggcagc
    21121 gtatcgtggg cggcacggtg actcagttca gactcgttca gtaccatccc ggcaaagaac
    21181 gcaccgagtg caaaggagac atcaaacagc tccaccgcac caaaggcaat ccctaacgcc
    21241 agcgccagca ccgacagggt aaacagctca cgggaaccgg ttgctgcgct acgtgccata
    21301 atccacggca ccagacggcg acctaccagc atcataatgg cgataaatgc gatcaccttg
    21361 ccgatggtga tccccatatc gacagcaaga gtggcaaagc ccacatcgcc ttgttccatc
    21421 attcctgcca ctgcgggcag caacaccagc gtcagaacca tgaccaggtc ttccacaatc
    21481 aaccaaccga tggcgatttg cccacgctga ctgtctatta attgccgttc ttcaagtgcg
    21541 cgcagtaaca ccacggtact ggcggtggaa aggcataagc cgaacacgat accggtcatt
    21601 aacgaccagc ccagcacggc ggagagcgcc atacccagca gcgtcgccac ggctatctgg
    21661 gcgatcgcgc cgggaatggc gatggccttt accgccatca aatccttcag cgaaaagtgc
    21721 agacccacgc caaacatcaa cagaatgacg cccagttcag ccagttccgg cgcaagcttg
    21781 gtatcggcaa caaagcccgg agtgaatggt cctgccagca cacccgctaa cagatatccc
    21841 accagaggag aaatacgtag tttattggcc agcatgccga ggataaaggc gagcacaagg
    21901 ccgccaacaa tggtggtgat aagcggggtg gcgtgatgca ttccgtctcc ttttcctggt
    21961 ggttattgtc catttttggc cgggaaaacc aaaattacag gtaatagttt atgacaattt
    22021 cattgatgat gtttatgaat aattgttgaa ttttgcagaa aaatggaatt agctgcaaaa
    22081 aaagcacgga tcggaaaaca gaaggcggtt taacagagga aaggcttatg gcgactgtaa
    22141 aacgatgcag ccaaagtttg cctttggctg caatgaaatc agtctttatg ccggttatca
    22201 ggcaggaata tggtcaacat ccccaatagt ggcaggaaag cacagatttt atagactaac
    22261 tcgatgctgg tgtgatcggc gataagcccc agaactgccg ctcccagacc tcccatgcca
    22321 aaagcaaaac cgaaaaagag tccagaaacc ataccgatac gtcccggaag tagctcctga
    22381 gcgtagacca gaatggcaga gaatgccgaa gcgaggataa atccaataat caccgttaaa
    22441 acccccgtcc agtgcaggct ggcgtagggt aaaatcagcg taaacggcgc aacgccgagg
    22501 atagagcccc aaatcacata tttccgtcca attttatccc ctacaggccc gccgatcacc
    22561 gtacctgccg caacggcaaa caggaaggca aacagatgaa gctgggcatt ctggatagat
    22621 aatccgaatt tttgcatcag ataaaaggtg taatagctgc tgatgctcgc catatagaaa
    22681 tatttcgaga aaatgaggat taacagaatg ctgaccgcca gtaccacttt gttgcgcggc
    22741 agtggattga taatcgtcgc tttgggtttt cctttattca ttcggtgctg tgccgagtac
    22801 caacggctga tttgcgccag caccacgatt gccagcagtg ccgcaagcac aaaccaggca
    22861 acgttgcctt tgccataagg cgcgataatc accgccgcca gcaagggtcc cagggaactg
    22921 ccaaagttgc cgccgacctg aaagatagat tgtgccaggc catgccgccc gccggacgcc
    22981 atacgggcca cgcgagaaga ttccggatga aagaccgatg aaccggtacc gaccagcgcc
    23041 gccgccagca gaactgcgcc aaaactgccc gccagcgcca gcagcaccag accacttaag
    23101 gtaaagcaca tgccaattgg caacgaccac ggcatcggat atttatcggt ccagtagccg
    23161 acaactggtt gcagtagcga agaggcgagc tggaaggtga gggttatcat gccaatctgc
    23221 ataaatgtca gagaaaactc tgactgaagc agcggataaa tcgccagaat cagcgattgg
    23281 atcatgtcgt tcagcagatg tgagaggctg atagcaccta aaataccaaa cgatgttcgg
    23341 gccttggtcg ttgacgcagc cgcgcccgcc acaggctggg tttgttcact cattgccata
    23401 ggaaagtcac tttttcaggg ttgcgatgta aagaatgatc ttatttgtga ttattacccg
    23461 actaacatac ctgtatgcgc cgcctgaagg aagtctcaac gctgaataca gaatttctaa
    23521 tctggatgca gatttatctt caccggacgc agacttgtct atgatgtcgc gtcatactat
    23581 ttttcaacac gttgaaatca ggtcagggag agaagtatga aattattgca gcggggcgtg
    23641 gcgttagcac tgttaaccac atttacactg gcgagtgaaa ctgctctggc gtacgagcag
    23701 gataaaacct acaaaattac agttctgcac accaatgatc atcatgggca tttttggcgc
    23761 aatgaatatg gtgaatatgg tctggcggcg caaaaaacgc tggtggatgg tatccgcaaa
    23821 gaggttgcgg ctgaaggcgg tagcgtgctg ctactttccg gtggcgacat taacactggc
    23881 gtgcccgagt ccgacttaca ggatgccgaa cctgattttc gcggtatgaa tctggtgggc
    23941 tatgacgcga tggcgatcgg taatcatgaa tttgataacc cgctcaccgt attacgccag
    24001 caggaaaagt gggctaagtt cccgttgctt tccgccaata tctaccagaa aagtaccggc
    24061 gagcgcctgt ttaaaccatg ggcgctgttt aagcgtcagg atctgaaaat tgccgttatt
    24121 ggcctgacga cggatgacac agccaaaatt ggtaatccgg aatatttcac tgatatcgaa
    24181 tttcgtaagc ccgctgatga agcgaagctg gtgattcagg agctgcaaca gacagaaaag
    24241 ccagacatta ttatcgcggc gacccatatg gggcattacg acaatggtga gcacggctct
    24301 aacgcaccgg gcgatgtgga gatggcacgc gcgctgcctg ccggatcgct ggcgatgatc
    24361 gtcggtggtc acccgcaaga tccggtctgt atggcggcag aaaacaaaaa acaggtcgat
    24421 tacgtgccgg gtacgccatg caaaccggat caacaaaacg gcatctggat tgtgcaggcg
    24481 catgagtggg gcaaatacgt gggacgggct gattttgagt ttcgtaatgg cgaaatgaaa
    24541 atggttaact accagctgat tccggtgaac ctgaagaaga aagtgacctg ggaagacggg
    24601 aaaagcgagc gcgtgctgta cacccctgaa atcgctgaaa accagcaaat gatctcgctg
    24661 ttatcaccgt tccagaacaa aggcaaagcg cagctggaag tgaaaatagg cgaaaccaat
    24721 ggtcgtctgg aaggcgatcg tgacaaagtg cgttttgtac agaccaatat ggggcggttg
    24781 attctggcag cacaaatgga tcgcactggt gccgactttg cggtgatgag cggaggcgga
    24841 attcgtgatt ctatcgaagc aggtgatatc agctataaaa acgtactgaa agtgcagcca
    24901 ttcggcaatg tggtggtgta tgccgacatg accggtaaag aggtgattga ttacttgacc
    24961 gccgtcgcgc agatgaagcc agattcaggt gcctacccgc aatttgccaa cgttagcttt
    25021 gtggcgaaag acggtaaact gaacgacctg aaaatcaaag gcgaaccggt cgatccggcg
    25081 aaaacttacc gcatggcgac attaaacttc aatgccaccg gcggggatgg ctatccgcgc
    25141 cttgataaca aaccgggcta tgtgaatacc ggctttattg atgccgaagt gctgaaagcg
    25201 tatatccaga aaagctcgcc gctggatgtg agtgtttatg aaccgaaagg tgaggtgagc
    25261 tggcagtaat ccgaaagtgc cggatgttcg catccggcac aatgcttaat cgcgacgggc
    25321 gatatcagca aatttggcat cgagtatctt tgccaggtcg cttgccgcca gttcgatatc
    25381 cagaccgcgc ttgccgccgg aaacataaat agtggcaaat tcttgtgcgg gggtgtcgat
    25441 aatcgttggc agacgttttt tctgccccag tgggctaatc cctccaacca gatatcccgt
    25501 cgaacgctgc gcgaccatcg gatcggccat ctcaactttc ttggcaccca gcgcttttgc
    25561 tactttttta agatccagtt gacctgcgac cggcgtaacg gccacggcaa ggtgtttcat
    25621 atcaccgttc actgcgacca gcagcgtttt gtagacctga tccggattca aacctaattt
    25681 tttgacgact tcatcgccaa aattggtttc agccggatcg tgctcgtagg tgtggatttg
    25741 aaacgaaatc ttgttttttt cgagtaattt aactgcgggt gtcatggtaa ttttcctgcc
    25801 ttaaacaaaa aaacgaagca agcatacgcc tgatggcggt ccaaaaaaaa gagtcatctt
    25861 gcctaagagt attggcagga tggtgagatt gagcgacaat cgagttacac cgtcgcttaa
    25921 agtgacggca taataataaa aaaatgaaat tcctctttga cgggccaata gcgatattgg
    25981 ccattttttt agcgcaacat ctgcggcaaa ttcccttctc catacaggtg tagtgcacca
    26041 accgcgacca catatcgccc cggcggcatg gcgcgtaatt tgtcccgcca ggcgagattt
    26101 cgctgatgca tcagcacatc gtacagcgac tgactgaacg tattaggcag cgttatttca
    26161 ttattttgtg gcggtgtatt cagccaccag ctcatcattt gttgcagcaa ccgtgcgttg
    26221 gtgtgccagt gggtcagcgt atcgtccagc agcgccagtc cgttgtcagg gagctggagc
    26281 aacatggcaa tctggttttc tgccccttcc agttcaatca cgggtttatg ttgttgcttc
    26341 gccgcctgca atagctggta atcgataccg tattctgccc gcagccccag tttttgtgcc
    26401 tgcgtcgcct gaagaaccat cgcgatttgc cacagcggtt gagtagaaaa gagcgacgga
    26461 gaaatgccca tctcctggct aatatgctgc agattttgga gttgctcctc gctaatgcgc
    26521 tcttccagcg tctcgcaggc aggcaaatta gcaaaaggcg tatcgctggt ggaaacatcc
    26581 gcctcgacga tcagcgcatc ggcgtttttg agctttttga gcaaacgggt gggcagggga
    26641 gccatatcgt ggctacccat atgaatactg ccaatcagat gaaaatggcg attgccggga
    26701 agagagatat cgatagcagg ccaggtgtaa tgattaccgc gcagcgcggc ccaaagcgtt
    26761 tttacccggt acaacagatc catacgacct ccctttgtga aatatcatgc tagcgcgcgg
    26821 ggagggatgg cgcaaatgga gttgattgtt gcacttgcgc acagtgcttg tgtgcattac
    26881 aatgattaaa taatggacaa ggagatgaga gaggaatggc tcaaaagaaa aatataagga
    26941 gttttcgtga tgcctggttg gctgattttt ttgtccattc aacgccacat cgaaagatcc
    27001 ctgctgaaat ccataccacg ttatcacgaa agttggacat cattaatgct gccacttctc
    27061 atcgggattt acgctcccct ccggggaacc gatacgaaga gttatccggg aaattacagg
    27121 aatactcctc cattagagtg aataaacagt accgactgat ttttaaatgg gttaatggca
    27181 aggccgaaga attgttttta gacccacaca actactaaaa catagctgtc gtattgacga
    27241 gaaaggtaaa actatgaaac aggcaacaag aaaaccgacg acacccggag atattcttct
    27301 ctatgaatat ctggaaccgc tcgatttgaa aatcaatgag ttagcagagt tgctgcatgt
    27361 tcatcgtaat agcgtcagtg cactgatcaa taacaatcgt aaactcacca ctgagatggc
    27421 attccgtctg gcgaaagttt ttgataccac agtcgatttt tggctaaacc tccaggcggc
    27481 agttgatctt tgggaagttg aaaacaacat gcgtacccag gaagaattgg gacggattga
    27541 aacagtggct gaatatttgg cacgccgtga agagcgtgca aaaaaggtcg cgtagaataa
    27601 tttccctata acggatgcgc caccctaaag cagtgcatcc gcaatgatgt acttattcct
    27661 tcggtttaaa ccgcagcaac cggttggcgt tactcactac ggtaatcgac gagagcgcca
    27721 ttgccgctcc ggcgaccacc gggttaagca gcgtaccggt gaacggccac aaaataccgg
    27781 cggcgaccgg aataccaatg ctgttgtaga taaacgcacc gagcaggttc tgcttcatgt
    27841 tgcgcagcgt tgcgcgggaa attgcgagcg catccgcaac acccatcagg ctatggcgca
    27901 tcagggtaat cgccgcggtt tcaatggcaa catcactgcc gccacccatc gcaataccga
    27961 catccgcctg agccagcgct ggcgcatcgt taatgccgtc gcccaccatt gctacctgac
    28021 gtccttcact ttgcagacgt ttgatcgctt cggctttacc gtctggcagc acgccggcta
    28081 tcacctcatc aatccctgct tctttagcga tcgcattggc ggtggtcggg ttatccccgg
    28141 ttagcatcac caggcgatat cccgctttat gcaggcgttg cagcgccgcc acgctatcac
    28201 tacgcaacgg atcgcgtaca gccagcaggg ctaccgcttt cccgtcaacc gccagcagca
    28261 caggcgttgc cccttgcgat gcctgagctg aaatatctgc ttcaattgct ttggtatcga
    28321 cctgttggtc atttaacagc gcctgattac ccagcaatac cgcatgacct tcagcttcac
    28381 cgctcacacc cagcccgcgc aatgtgcgga aaccgttgac ctgcggcagc tgcatatcac
    28441 ctgctttatc gaggatcgcc cgtgccagcg ggtggctgga accttgctcc agcgccgccg
    28501 ccagacgcag cgcctgcgct tcatcaacat cagcaaatgt ttttactgcg acaacctgcg
    28561 gcttcccttc ggtcagcgtc ccggttttat cgaacaccac ggtgtcgagc gtactggcgc
    28621 gttgcagcgc gtcggcgtcc cgcaccagca cgccaaactc agccgcccgc ccgacgcccg
    28681 aaataatcga catcggcgtc gccagtccca gcgcacacgg acaggcgata atcagcaccg
    28741 tggtggcaat caccagggta taaacaattt gcggtgccgg gccaaagaaa taccagattg
    28801 ccgcactgac cagcgcaatg actaccacca ccggcacaaa cacagcggag attttatccg
    28861 ccagttgacc gatttctggc ttgctgctct gggcctgtcg caccatgcga atgatccgtg
    28921 acagcgtagt atggctgccg accgcactgg cgcgaaacag cacactgccg tcctgtacca
    28981 ctgtcccggc atggacgcta tcgccttcgc ctttttgctg cgggattggt tcgcccgtca
    29041 gcatcgcttc atccagccac gcttcgccct gggtaatttc accatctacc ggcacccgat
    29101 cacccgtcgt caggcgcagc aacatacctg gctgaacatc tgccagcggc acgttttttt
    29161 caccttcgtt ggtgaccagg cgcgccgtcg gtggggttaa atcgagtaat ttctccagcg
    29221 ccttagaaga acgctgacgt gcgcgcgctt ccagcatatg accgagatta atcagaccga
    29281 taatcatcgc actggcttca tagtaaagat gtcgcgcttc catcgggaac cactgcggcc
    29341 acaggttgac gctcattgaa tagagccacg ccacgcccgt acccagcgcc accagcgtat
    29401 ccatcgtcgc tgcaccgttc agcaggcttt tccatgcgct gcggtaaaaa tggccgccgg
    29461 cgaaaaccat taccgccagg gttatcaggc cgataaccaa ccacaggctg cggttgtcgg
    29521 cggtgaccat catgttatcg ccgatcatcc cccagaccat caccgggata cccaccgcca
    29581 gtgcgacaat cgcctgccag cggaagcgct tcatcgtagc gacggcggtt tcttgctggc
    29641 gctcgcggcg tttagcgtca tcttcaatcg cttccgcgcc gtagcccgct ttttccaccg
    29701 cctgcactaa atcttgtggg gaggcactgc ccatcaccag cgcagtacgc tccgccaggt
    29761 ttacccgtgc ctgagtgacg cccggtacgc tttgcaacgc attttgtacg cgggtgacac
    29821 agctggcgca gctcatgccg ctcagcagca actgctggct gtcatcgtca tcggcggtcg
    29881 ctgccggaag cgcctcagaa accgctgtca gtgcttccga cgggattgat gactccgcca
    29941 gcggtttagc ctttgggtgg cttacagatg cgtcataacc cgcttgtttg atggtttcga
    30001 tcagctgttc tgcactggca gtcccggtaa cgtgcgcttc agtgacagac acatccgcct
    30061 gctcaacatc cgggcgctgt tcgagacttt ctttcacgcg tttaacgcag tgaccgcagg
    30121 acaggccgtc cagggtcagg tcgatagttt gtgacataaa acactccttt taagacagtt
    30181 ttgactggct gtgataaagg ttaaaccttc cagcaagggg aaggtcaaga aattaataaa
    30241 ccaggcgggt aaaagtccgt aaagattaaa aaatcggctc gatttgcatc aggattagac
    30301 atttatctct ttgttttcct gtagttaagt tgcgggtgct aagttaaatc aggatgcctg
    30361 aaaattggca ccggggtgag gaattacctc ccgcatctat aaaaaggagt taacaaaaga
    30421 tgttagatgc aaacaaatta cagcaggcag tggatcaggc ttacacccaa tttcactcac
    30481 ttaacggcgg acaaaatgcc gactacattc cctttctggc gaatgtacct ggtcaactgg
    30541 cagcagtggc tattgtgacc tgcgatggca acatctatcg cgcgggtgat agtgattacc
    30601 gctttgcgct agaatccatc tcgaaagtct gtacgttagc ccttgcgtta gaagatgtcg
    30661 gcccgcaggc ggtacaggac aaagttggcg ctgacccgac cggattgccc tttaactcag
    30721 ttatcgcctt agagttgcat ggcggcaaac cgctgtcgcc actggtaaat gctggcgcta
    30781 ttgccaccac cagcctgatt aacgctgaaa atgccgaaca acgctggcag cgaattttac
    30841 atatccaaca acaactggct ggtgagcagg tagcgctctc tgatgaagtc aaccagtcgg
    30901 aacaaacaac caacttccat aaccgggcca ttgcctggct gctgtactcc gccggatatc
    30961 tctattgtga tgcaatggaa gcctgtgacg tgtacacccg tcagtgctcc acgctcatca
    31021 atactgttga actggcaacg cttggcgcga cgctggcggc gggtggtgtg aatccgctga
    31081 cgcataaacg cgttcttcag gccgacaacg tgccgtacat tctggctgaa atgatgatgg
    31141 aagggctgta tggtcgctcc ggtgactggg cgtatcgcgt tggtttaccg ggcaaaagcg
    31201 gtgtaggtgg cggtattctg gcggtcgtcc ctggcgtgat gggaattgcc gcgttctcac
    31261 caccgctgga cgaagaaggc aacagtgttc gcggtcaaaa aatggtggca tcggtcgcta
    31321 agcaactcgg ctataacgtg tttaagggct gatcatgatg aacacggaag gtaataacga
    31381 taacaaaccc ctcggtctat ggaacgtcgt ttccatcggt attggggcaa tggtgggggc
    31441 ggggatcttc gcgctgctgg ggcaggctgc gttgctaatg gaagcctcga cctgggtcgc
    31501 ctttgctttt ggcggtattg tggcgatgtt ttccggttat gcctatgcgc gcctaggggc
    31561 gagctatccc agcaatggcg gtattatcga cttctttcgt cgcggattag gcaacggcgt
    31621 cttttcgctg gcactctcgt tattgtacct gttgacgctg gcggtgagca tcgccatggt
    31681 cgcccgtgct tttggcgctt atgccgtgca gtttttgcat gaaggcagtc aggaggagca
    31741 ccttatattg ctctacgcgt tggggatcat tgcggtgatg acgcttttca actccttaag
    31801 caaccatgcg gtagggcggc tggaagtgat cctcgtcggc attaaaatga tgatcctgtt
    31861 attgctgatc attgccggtg tctggtcgct gcaaccggcg catatttccg tctctgcgcc
    31921 ccccagctcc ggtgcgttct tctcctgtat cgggataacc ttccttgcct atgcgggctt
    31981 tggcatgatg gcgaacgcgg cggataaagt gaaagatccg caggtcatta tgccacgggc
    32041 gtttctggtg gcgattggcg ttaccacgtt gctttatatc tcgctggcgc tggttttgct
    32101 tagcgatgta tcggcattag agttagaaaa atatgccgat accgccgtag cgcaggcagc
    32161 ttctccgctg ctcgggcatg tgggttatgt gatcgtcgtc atcggcgctt tactggcgac
    32221 ggcttcagcc atcaacgcga acctgttcgc cgtgtttaac atcatggaca acatgggcag
    32281 cgaacgcgaa ctgccgaagc taatgaataa atccctgtgg cggcagagta cctggggcaa
    32341 catcattgtc gtggtgttga ttatgctgat gacggcggcg ctgaatttag gctcactcgc
    32401 cagcgttgcc agcgccacct ttttgatttg ctatctggcg gtgtttgtgg tggcgatccg
    32461 cctgcgtcat gatattcacg cctcgttgcc gattcttatc gttggtacgt tggtgatgtt
    32521 gttggtgatc gttggcttta tctacagcct gtggtcccag ggcagccgtg cgctgatatg
    32581 gattattggc gcactcttac tcagccttat tgtggcaatg gtcatgaagc gcaataaaac
    32641 cgtataacat ctctctgtgc gcagtacttc ctgtattatt gtggtggcgg tcgatattcg
    32701 cactggcaaa aaaacgtgct tgaatatttg ttgaaaccct ttaacaaagc acaggaggcg
    32761 ttgcgcgaac gatgaacatt agcgatgtag caaaaattac cggcctgacc agcaaagcca
    32821 ttcgcttcta tgaagagaag gggctggtga cgccgccgat gcgcagcgaa aacggttatc
    32881 gcacctacac gcagcagcat ctcaacgaac tgaccttact gcgccaggca cgccaggtgg
    32941 gctttaacct ggaggagagc ggcgaactgg tgaatctgtt taacgatccg cagcgtcaca
    33001 gcgccgacgt caaacgtcgt acgctggaga aagtagcgga gatcgaacag catatcgaag
    33061 aactgcaatc catgcgtaac cagctgctgg cgctggcgaa tgcctgcccc ggtgatgaca
    33121 gcgccgactg cccgattatc gaaaatctct ccggctgctg tcatcatcgg gcggggtgat
    33181 taaggcttgc gaaaatagtc gtccattatt ttcgtcagct ccggacgttc cccgtcatca
    33241 ttgatggcaa aatccagaaa aaaagcatct gcttgcggcg ttcggttttg cgccatcaac
    33301 cggaaggtga gggggcctga ccaggtttcg gatgggctga tgtgcttatc agggccgaaa
    33361 ctctcccatg cgttgggcgg taatgttttg cacgcggtga gttttggtat gtggtgttgc
    33421 agctctgttt tgtgcagaca ttccccgcca ggaaatacca gggtttgttg ctcatcatta
    33481 aaatgggttg ccgtgctgag cacctgtgtt ttgagcggtc gcccttccag cgcggcggca
    33541 gtagccttat ggtgtccgtc gagcagaacg cagagatagg aatcagggag ataccaggcc
    33601 agagcgcggg cgattccagt tttcgagagc agacaaaaac cgggcggctc cctgttcacc
    33661 ctccgcaaaa ccgctgtaga caaattccgc acaggtaggg caaccagcct cggaccagaa
    33721 acgtattaac gggacatcct tgccagcaga gcgaatggat aagaccgtgc gagtgcggat
    33781 cgtaaatcac acattgacgc agaaatcctt tcccggactt caccgaaaaa atgacgcccg
    33841 cagcggtatt tttcagcaga cgtacttcag gttttcgaaa ggggaaaaag catggccact
    33901 ccttagccaa aatttcagtt ccctgaactg tgccagaaaa ttacgctggt gactattgct
    33961 ctggaaatgg aaacggtgtc cttacccatt aatccccgtt tgtaccgaaa gaatatgttg
    34021 caaactttct accataatgc gccccgactg aaataagcct tcctggattt cattgttaac
    34081 attcacagcg tgaaccaggg aggctaataa caccacttgc aagaaggcaa aaataaacgc
    34141 accgataatc ataatatgcg tcagggggat tttcctttgc tccttcattt taaataaccc
    34201 acaaactggc cttctttcat taaggcgact tttcctgtgt gatgataaac aatgacatta
    34261 tacagacccg actgataaac cgtggcatcg gcattatctt catctatggc acataatgaa
    34321 tcattgagat aagcaaaatt cttactactc attctaaagg tcatgccaaa tcctttataa
    34381 atggcttttt ttgccgcctg ggcatgttgt gcggcttctg catttttaaa tgcctgcgct
    34441 tcagaagcac ttaaaatagc cgtctggcgc tgctgttctg cgagataaca ggtgtcatgg
    34501 ctgacgccct gcgcctgaca atctgcgatg cgggaagcgg gtgagctaca gccagccagt
    34561 aatataaccg ccagcaggga aaaggattta atggagctat tcatcattgt taaccttaaa
    34621 taatgtgcga attaatgaag aggtgaaacc cggcaagctt ccttgctctt atttatattg
    34681 aggattgaaa tattagaacg aatatcctac gccgacacct gcgccgaaat cagattgcgt
    34741 atccgcagaa ataccggctt taagtaccgt atgcgttccg gcattaaaag atgctccgac
    34801 agacaccgca gattcaccgt tgaaggttcc cgctcccgct gacaccatca tcacgtcacc
    34861 ggttttaacc tgtggttgtg aggcgatagc gatggcagat gcagttcccg cattggcttc
    34921 tttacggttg tcctcaactt cgtttttcag cgaactgaaa tggttattga gcgtgttaat
    34981 ttgttggctg ttatctgcga cccgtttttg cgtgttgctt aactgttgct gcgtttgtgc
    35041 ggaatcttgt ctcagacttt caatttgctc atgtgcctgg acagacttct gcgccagact
    35101 ttgcatttgt ttatcgtatt gagccctttc tttttgctgt tgtgctgcca catcggttgt
    35161 cagtgcattc attttctgcc cttgttccgt ggtagtgcgc tgattagcct cattttgagc
    35221 cgccagtctg gcattaatca tctcctgata atgcgcagta ttttgcgtat ctccagtcgc
    35281 ggcgagcgtt ttttgtgtgg ctacgatctg cgcctgttgc tctgtaattt gcgtttgctg
    35341 ggcatcagtt accgcagact gtcgggcgtt gatcatgctc tgataacggg caggtgtctg
    35401 tgcatcacct gtagcggcaa cggttttttg tcctgcgtca ggatctgtct ggacagtggg
    35461 agtctgagtg gtatcaggtg tttcagaagg gatgtagtga tgttcagtca aataggtata
    35521 cgcagcgggg ctgttgtctg tgcgttctaa aaagcgggta atcttctgct caatctccga
    35581 ttgcgacatg ccagagttaa tggctgaaag ctctgttgcc gccgcttcaa tggtatcacc
    35641 agctaaaaca acagggctgg atgttgctga aattatgagt gccagtaaag ctacgtttac
    35701 agttttcatt cattttctct tgttcaaaat aaatcgcatt aatgtttcta taagaaacaa
    35761 ataatattat tttaagaaat aaactgcgtt gcaatttaat cgaaa
//
LOCUS       DAPHCV010000046        35620 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.046, whole genome shotgun sequence.
ACCESSION   DAPHCV010000046 DAPHCV010000000
VERSION     DAPHCV010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.046"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..621)
                     /locus_tag="RH033_002206"
     CDS             complement(<1..621)
                     /locus_tag="RH033_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDV2991385.1"
                     /translation="MIIEKVMNNNCVQASMNGQEVIISGPGVGYNKKYGMSVPEHPAN
                     RIFYVRNEQKNKLYKLIEHVDIEYVFVAEKIVQYAENNLEKNLNPSLLLILADHISNA
                     ISRVASGIQINNVFLDEIKALYKAEYAISRDALTIINEQFSVQLPDDEIGFIALHILN
                     NYENSVDYESVRIIELSQIITGLIEVVYNRKVDRSSFNYSRFMIHLK"
     gene            complement(879..2132)
                     /gene="amiC"
                     /locus_tag="RH033_002207"
     CDS             complement(879..2132)
                     /gene="amiC"
                     /locus_tag="RH033_002207"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HDV2991386.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            2364..3695
                     /gene="argA"
                     /locus_tag="RH033_002208"
     CDS             2364..3695
                     /gene="argA"
                     /locus_tag="RH033_002208"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HDV2991387.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(3933..5759)
                     /gene="recD"
                     /locus_tag="RH033_002209"
     CDS             complement(3933..5759)
                     /gene="recD"
                     /locus_tag="RH033_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HDV2991388.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     SHVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTVVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(5759..9301)
                     /gene="recB"
                     /locus_tag="RH033_002210"
     CDS             complement(5759..9301)
                     /gene="recB"
                     /locus_tag="RH033_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HDV2991389.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDAITAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVDEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDSLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYATRPNAGLIDLMDEMFAGITLEEA"
     gene            complement(9294..12182)
                     /gene="ptrA"
                     /locus_tag="RH033_002211"
     CDS             complement(9294..12182)
                     /gene="ptrA"
                     /locus_tag="RH033_002211"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HDV2991390.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(12358..15726)
                     /gene="recC"
                     /locus_tag="RH033_002212"
     CDS             complement(12358..15726)
                     /gene="recC"
                     /locus_tag="RH033_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HDV2991391.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDTETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQLGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(15739..16062)
                     /locus_tag="RH033_002213"
     CDS             complement(15739..16062)
                     /locus_tag="RH033_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDV2991392.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRANSPPAHWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(16047..16454)
                     /locus_tag="RH033_002214"
     CDS             complement(16047..16454)
                     /locus_tag="RH033_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HDV2991393.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSTLEWGKMHSWQTQPAVQCLLYAATGARVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(16451..17014)
                     /locus_tag="RH033_002215"
     CDS             complement(16451..17014)
                     /locus_tag="RH033_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HDV2991394.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(17005..17475)
                     /locus_tag="RH033_002216"
     CDS             complement(17005..17475)
                     /locus_tag="RH033_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HDV2991395.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISLIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(17660..18454)
                     /gene="thyA"
                     /locus_tag="RH033_002217"
     CDS             complement(17660..18454)
                     /gene="thyA"
                     /locus_tag="RH033_002217"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDV2991396.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(18461..19336)
                     /gene="lgt"
                     /locus_tag="RH033_002218"
     CDS             complement(18461..19336)
                     /gene="lgt"
                     /locus_tag="RH033_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDV2991397.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(19487..21733)
                     /gene="ptsP"
                     /locus_tag="RH033_002219"
     CDS             complement(19487..21733)
                     /gene="ptsP"
                     /locus_tag="RH033_002219"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDV2991398.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(21746..22276)
                     /gene="rppH"
                     /locus_tag="RH033_002220"
     CDS             complement(21746..22276)
                     /gene="rppH"
                     /locus_tag="RH033_002220"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HDV2991399.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            22961..23650
                     /gene="mutH"
                     /locus_tag="RH033_002221"
     CDS             22961..23650
                     /gene="mutH"
                     /locus_tag="RH033_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HDV2991400.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGVRGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            23719..24432
                     /locus_tag="RH033_002222"
     CDS             23719..24432
                     /locus_tag="RH033_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDV2991401.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            24570..24788
                     /gene="ygdR"
                     /locus_tag="RH033_002223"
     CDS             24570..24788
                     /gene="ygdR"
                     /locus_tag="RH033_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HDV2991402.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            24896..25936
                     /locus_tag="RH033_002224"
     CDS             24896..25936
                     /locus_tag="RH033_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HDV2991403.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALTEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(25968..27161)
                     /gene="lplT"
                     /locus_tag="RH033_002225"
     CDS             complement(25968..27161)
                     /gene="lplT"
                     /locus_tag="RH033_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HDV2991404.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLISMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLSAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(27154..29313)
                     /gene="aas"
                     /locus_tag="RH033_002226"
     CDS             complement(27154..29313)
                     /gene="aas"
                     /locus_tag="RH033_002226"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HDV2991405.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTKALKGERVLITPNHVSFIDGIL
                     LALFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPFDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            29899..30930
                     /gene="galR"
                     /locus_tag="RH033_002227"
     CDS             29899..30930
                     /gene="galR"
                     /locus_tag="RH033_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HDV2991406.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETIGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(30937..32199)
                     /gene="lysA"
                     /locus_tag="RH033_002228"
     CDS             complement(30937..32199)
                     /gene="lysA"
                     /locus_tag="RH033_002228"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDV2991407.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVLEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     AGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            32321..33256
                     /gene="lysR"
                     /locus_tag="RH033_002229"
     CDS             32321..33256
                     /gene="lysR"
                     /locus_tag="RH033_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HDV2991408.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERIRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFHGENYISLSRTDSYRQLLDQLFTENQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(33243..33935)
                     /gene="ygeA"
                     /locus_tag="RH033_002230"
     CDS             complement(33243..33935)
                     /gene="ygeA"
                     /locus_tag="RH033_002230"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDV2991409.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCSLPFL
                     HIADATGCAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAT
                     IHAEDAVAFMLS"
     gene            complement(34064..35482)
                     /gene="araE"
                     /locus_tag="RH033_002231"
     CDS             complement(34064..35482)
                     /gene="araE"
                     /locus_tag="RH033_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HDV2991410.1"
                     /translation="MVTINTESALTPRPLRDMRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         8786 a         9698 c         8771 g         8365 t
ORIGIN      
        1 tttaaggtgt atcatgaacc tggaataatt aaatgaactt ctgtccacct ttctgttata
       61 aacaacttca ataagccccg tgattatttg cgacaactca ataatccgta ctgattcata
      121 atcaactgaa ttttcataat tatttaagat atgcaatgct ataaaaccaa tctcatcatc
      181 aggaagttga acgctgaatt gctcattaat gatagttaat gcatcgcgac ttatcgcata
      241 ctctgctttg tacaacgctt tgatttcatc aaggaaaaca ttattaattt gtatgcctga
      301 agcgactcgg gatattgcat tcgaaatatg atcagcaaga atcaatagta gcgatggatt
      361 gagatttttt tcgagattgt tctccgcata ttgcactatt ttttcggcaa caaaaacata
      421 ctcgatatcc acatgctcaa tcaatttgta gagtttattt ttttgttcat ttctgacata
      481 aaaaatccgg ttagccggat gctcagggac cgacattcca tattttttgt tgtaaccgac
      541 gccaggccca gaaatgataa cctcctgtcc attcatcgat gcctgtacac aattattgtt
      601 catgactttt tcgatgatca ttccatagct ccaaaaaaaa aggcaaggca cgtcagaagt
      661 aataactact tcaaacattg ccttgcctga tttaacagtt acaagatgat taactaatgc
      721 atatacatta cacggaattg tgcttaatta aagagatggt cggcttattt ttcaagccga
      781 cattcaaata ttcgacatca gtcacaaaac caacgtctaa tattcaggta aatattatct
      841 attcaacgaa atataaattc attgaataaa taaaaacatt atccccttct cgccagcgtc
      901 gccccatcgg caaaatacgc ctttatccct gcgaggattg attccgcaac ttcctgctgg
      961 aaggttgccg ttttcagttt acgttcttcc tcaacgttac tgataaacgc cgtttcgacc
     1021 aaaatggagg gtatatcagg cgctttcagc acggcaaacc cggcttgttc aacctgattt
     1081 ttatgcagct tgttgatttt accgagctta ttcagcaccg ctttaccaaa cttcaggctg
     1141 tcggcaatgg tcagcgactg caccatatcg aacatggtgt ggtcgacata gcggtcaccg
     1201 cttttgctca cgccaccaat caagtccgag gcgttctggg tttgtgccag atattttgcc
     1261 gcagtactgg ttgcaccttt ggttgagagc gcaaacacag aggaaccgct cggctgacga
     1321 ctggtaaagg cgtcggcatg gatagagaca aacaggtcgg cacgctgttt ctgggctttt
     1381 gctacgcgca cttgcaacgg aatgaagatg tcttcattgc gcgtcatgta caccttcata
     1441 ttgccctctt tctcgatcag agagcgcaga cggcgagcta tttgcaatac cacgtctttt
     1501 tcgcgcgttt tgtatttccc caccgcaccg gagtcttcgc caccgtggcc agggtcaagc
     1561 ataatgacaa tcggacgatc gcgccctgct ttacctggtt gtggaccact ttgcgctggc
     1621 ggcacctgct tttcgaggtc gcctttgttg taatcctcca gcagcgccag cagcgggtcc
     1681 tgcatatcct gtgcattggc cggatagagg tccatcacca gacgctcttt aaacccggcg
     1741 accggcgcaa gggcaaacag ctgcggtttt acgttttgct ttaattcaaa aaccatgcgt
     1801 acggtttgcg ggtcaaattg cccgacgcgc gccgacttga tgaacgggtc gtctgcacga
     1861 atttgtgccg ccatcccctt gagtaccgag ttcaggttta catcttcgat atccaccacc
     1921 acgcgttccg ggttactcaa cgcgaactgc ttatatttca gctgacgatt tgattctacc
     1981 gtcacgcggg tgtagctgga cgcaggccag acgcgcaccg ccacgacctg gctgaccgca
     2041 gccaggctga cctgacttac gctcaatagc cacatggcac ccgcgccttg cagtaaacga
     2101 cgacggctga ttgcagtgtt ggatcctgac atgcctctcc cgagcaaaag aaatctaagc
     2161 tgtgtaacaa gtaaacgacc aatttgaccg gaaactttag cgaaagacgc ataatctgtc
     2221 atctataaaa cggtaaacat tctttttata ttcacggtat tactgataaa aaactctctc
     2281 ccgcataaaa tttacacttg caccctgcga aaaaacagaa taaaaataca ataatttcga
     2341 ataatcatgc aaagaggtgt accgtggtaa aggaacgtaa aaccgagctg gtcgagggat
     2401 tccgccattc ggttccctat atcaataccc accggggaaa aacatttgtc atcatgctcg
     2461 gcggtgaagc cattgagcat gagaatttct ccagtatcgt taatgatatc gggttgttgc
     2521 acagcctcgg catccgtctg gtggtggtct atggcgcacg tccgcagatc gacgcaaatc
     2581 tggctgcgca tcaccacgaa ccgctgtatc acaagaatat acgtgtgacc gacgccaaaa
     2641 cactggaact ggtgaagcag gctgcgggaa cattgcaact ggatattact gctcgcctgt
     2701 cgatgagtct caataacacg ccgctacagg gcgcgcatat caacgtcgtc agtggcaatt
     2761 ttattatcgc ccagccgctg ggcgtcgatg acggcgtgga ttactgccat agcgggcgta
     2821 tccggcggat tgatgaagac gcgatccatc gtcaactgga cagcggtgca atagtgctaa
     2881 tggggccggt cgctgtttca gtcactggcg agagctttaa tctgacctcg gaagagattg
     2941 ccactcaact ggccatcaaa ctgaaagctg aaaagatgat tggtttttgc tcttcccagg
     3001 gcgtcactaa tgacgacggt gatattgtct ccgaactttt ccctaacgaa gcgcaagcac
     3061 gggtagaagc ccaggaagag aaaggtgatt acaactccgg tacggtgcgc tttttgcgtg
     3121 gcgcagtgaa agcctgccgc agcggcgtgc gtcgctgtca tttaatcagt tatcaggaag
     3181 atggcgcgct attgcaagag ttgttctcac gcgacggtat cggtacgcag attgtgatgg
     3241 aaagcgccga gcagattcgt cgcgcaacaa tcaacgatat tggcggtatt ctggagttga
     3301 ttcgcccgct ggagcagcaa ggtattctgg tacgccgttc tcgcgagcag ctggagatgg
     3361 aaatcgacaa attcaccatt attcagcgcg ataacacgac tattgcctgc gccgcgctct
     3421 atccgttccc ggaagagaag attggggaaa tggcctgtgt ggcagttcac ccggattacc
     3481 gcagttcatc acggggcgag gttctgctgg aacgcattgc cgctcaggcg aagcagagcg
     3541 gcttaagcaa attgtttgtg ctgaccacgc gcagtattca ctggttccag gaacgtggat
     3601 ttaccccggt ggatattgat ttactacccg agagcaaaaa gcagttgtac aactaccagc
     3661 gtaaatccaa agtgttgatg gcggatttgg ggtaagtggt ggaaatcgcc agatgcgaca
     3721 tgcgcaatgc ctggaggcat gataaacacg ctagcatcgc atcaggtatt gacgctcatc
     3781 gtcggatgcg gcgtgaacgc cttatccgac ctacatcaaa tcaaaggtgt tgatggcgga
     3841 tttagggtaa gcgacggaaa tcgccagatg cgcatatgcg caatgcccgg aggcatgaca
     3901 ggacgcgcat gcgtcgcatc aggcaatcac gtttattccc gcgaactaaa caacgccgcc
     3961 agaccactgc gccgctcagt acgagtggcg attgccgcac ttaatatgcg ctcatcggca
     4021 tacagcgaca gacggcgacg cgcgcgggtc actgcggtgt aaaccagctc tcgcgttact
     4081 accggcgtgc gttgactcgg taaaatcaac gccgcatggt cgaactccga tccctgcgat
     4141 ttatgtaccg tcatcgccca cgtcgtttcg tgctctggca ggcgactcgg ttgcacagac
     4201 ttaatattgc cgtccggcat cgcaaaccag acgcgcgtcc cctgcccgcg atccagcgca
     4261 ataccaatat cgccattaaa caacccaagc gcgctgtcat tacgggcaat catcaccggt
     4321 cggccttcgt accaacgaga gtgcggatga cgatgaattt tgcgcttctg ttgcataaac
     4381 tgctcaattc gctcattcag tccagccacg ccaaacggcc cttcccgcag ggcgcacaaa
     4441 agctggtact cattgaacgc ctgaatgatt aaatccggct cggcacgcgc ttgcaacaga
     4501 tccagataac gtccgtaacc cgcaagagct tcctcaagca tcgcaatata atcttcgccg
     4561 ctctgtaaaa gtcgtttttc gatatcagta aaatcctgct gaaaaacggt tttcactacc
     4621 gttttatcac cacggttaat cgccgcagct aactgaccaa tgccagaatc gctgccgaaa
     4681 cgatagcttt tttgcagcag gcagaggctg tcgcgcaaag atgccgcttc tgtgccagtt
     4741 cctgctggaa cgtggcttcc cgtcaggcgg ctcaactgcc cggcacgctc ggcggtaaag
     4801 cccgcgttgg cataagcgca gatatcgccc agcacagccc cagcctcaac cgaggccagt
     4861 tgatcacgat cgccgagaaa gatcactcgc gcatgatcgg gcaaggcgtc gatcagtctc
     4921 gacatcatag gcagatcgat cattgacgct tcatctacca ccagcacatc aagatgcagc
     4981 gggttaccgg catgatgacg taaacgctgg ctacccggct gcgcacccag caatcggtgc
     5041 aaagtgctgg catcttccgg aatgcgtttc ttttgttcat cggtcagcgg taattgtcgc
     5101 aaagccttgc cgagagattc ggttaagcgc gcggcagctt tacccgttgg tgcagccaga
     5161 cggatacggc agcgttctcc gtcggccatt tgaattaagg ctgccagcaa cttcgctacg
     5221 gtggtcgttt taccggtgcc aggaccgccg gaaatcaccg aaatccgccg cgtcagcgcc
     5281 actgccgccg caactttttg ccagttaatt tcatcgctta ctggaaaaag tttgtccagg
     5341 gtttgcgcca gtagagcttc atcaacctca atggcatgat tcacttcgtt gaaaaagcgt
     5401 gccactgtgc gctcgttaca ccacatgcga ttcaaataaa gacgatcacc acagaggatc
     5461 atcggcgttg gttcatctcc ccggctgacc gcttgtgaag ccagcaagca ttcttcccaa
     5521 ttttgtaact caccgatttc gctgacgcag gttgctaaca gcggatgtga cgcctcgtta
     5581 ttttccagtc gtgagagcgg taaacagaca tgcccttccc cggcgtcgtg gcttaacagg
     5641 gctgccgcga gggtgacggc aggatgttca tctcccgcca cggtcagggc aaattgcaca
     5701 tccagcgggc gtaactgttt gtgctccaca gcttccagta attgcttttg caatttcatt
     5761 acgcctcctc cagggtgata ccggcaaaca tctcatccat caggtcaatc aacccggcgt
     5821 tgggtcgggt cgcgtagatc ccttgttgcg gatgttcttt atcaacacca cgcaggaaca
     5881 gataaataac gccgccaaag tggcgctcat agtcgtaatc agcaatgcga tggcgcagat
     5941 aacggtgcag cgccagggta taaagctgat attgcagatc atagcggtgt gcctgcattg
     6001 ccgctgccat agcctgttgg gtgtaagcag aactgtcttc acccaaccag ttggatttat
     6061 agtcgagcag gtaataacgc ccttcgtggc ggaacaccag gtcaataaag ccttttaaca
     6121 tgccgcgtac ctgcatgaac tccagcggcg ggcagccagc ggatagcgag tcaaactggc
     6181 gaattagtgc atcaagctgg ctggcgatga gcggttcgct aatcggcagg taaaactcca
     6241 tctccacctg tttatcgcgg tcggaaagct gactcaggct aacacccgtt tcattgagag
     6301 gtgcctggag gacagccgtg atccactcgg tcaacaccgg ttcccactgc gattcaaagc
     6361 cgccgagttc cagtttttcc tgtacccagt tcgggtcaac cggctgggta aaatcgaggt
     6421 cttcaaacaa actgtgcaag aatgtccccg gtgacgcacc gcgcgggaac tgatgcggtg
     6481 ttaacgtcgg ttcgtcaacg acgctgacca cgcctgcggc atcaacatcc agccgtggca
     6541 tcagatcctg agcgataccg tgaccacgct gctgcaaacc ggagtagctg gtgacacgcc
     6601 agttatcgcc gggcaatcgt tgtagcgtcc tcgcattcag ttctgcagtt atagcatcat
     6661 taacctgcca gggctggtta tcaccagttt gtgccgtttg ccaggcaata tcatcatcgc
     6721 ataacgcttc aatacaggtg cgaagccctg ccgcatcttg cggttcccct ttttgcagca
     6781 aacgcccgag cgcactttgg tggacgtcgg tgtcaccttt tttatcgcca cgacggcgca
     6841 ccagcggtgc aacgccgaga ctgcaatgcc aaaccgaacg cgtcagcgcc acgtaaagca
     6901 aacgcagatc ttccgccaga cgttcggcct ccgcgagatc gacgctttct ggcgcagcgt
     6961 taagatccaa aactgcctca aacgagtggc gatcgtgata gaacgcctga tcctggacgc
     7021 ggaaattggt aataaatggc aaccagacca gcggatattc cagccctttt gatttatgga
     7081 tcgtgacaat ctgcaccaga tgtttatcac tttcgaggcg catttgttgg ctggaggcat
     7141 tactgtctgg ctcgaggata tgttgcgata accagcgtac cagcgcatgt tcactttcca
     7201 gctgcgttcc ggcttcttgt agcaattcgc tgatatgcaa gatatcggta agacgccgct
     7261 caccgcctgc tgttgccagc aagttttctg caatgttacg cgccgacatc agcgcccgca
     7321 gcatcggcat aacgccacgt ttgcgccaga tttgccgata accatcgaac tcttcgacga
     7381 ccgcatccca cgcatgttcg tcattattca gcgtttcaat atccagcgca ttcagcccca
     7441 tcattgacgt tgccagcgca ctgcgcaggg tgttctcacg ttcgggagtc atcaccgcct
     7501 gcaacagcca aagcatctcc tgcgcttcca gagtttcaaa aacactgtcg cggttcgaaa
     7561 ggtaaacgga agggatttcc agcagcgtta aggcatcgcg cacctgggcg gcctcctggc
     7621 ggctgcgcac cagcacactg atgtccgaag cacgcaccgg acgcgcgtcg tcgccgttca
     7681 tcagcaacgc ttcgccccgc tgtccggctt gtagccagtc gcggatttgc gcagcacata
     7741 cctgcgccat ggtactttga tagtcgccaa cgccgcagct ttcgccttcc atcagccaca
     7801 ttttcatcgc aggctgcgtt tcacctttaa acacaaaacg taacgcctga tttttgccgg
     7861 cagatttcac aggaataaac ggtatttcac gaaacatgaa cgcgtcatca gtctggctga
     7921 aaagtttgtt aacgctgttc accattcccg gtgcggaacg ccagttggta tctaaggtgt
     7981 agtgagcatg aacttcgcta cgcgccttca tataagtgaa gatatccgca ccccggaatg
     8041 cataaatagc ctgtttcggg tcgccaatca gcaacaatgc ggtttccggc tgatggtgcc
     8101 agatacggcg aaaaattcgg tactgctggg ggtcggtatc ctgaaattca tcgatcattg
     8161 ccaccgggaa tcgcgtacgg atcgccgctg ccaatacctc gccgctttca ctacgcagcg
     8221 cggaatcgag ccgacttaac atgtcatcaa aacccaattc gccacggcgg cgtttttcac
     8281 gcgctactgt ttcgcggatc tcagctaatg cgcgagtgat caccagatcg cggatcgaca
     8341 atggttctgc aagcagttga tcgatcgcct caaacagtgg atgtcgcggg gtttcccccc
     8401 cggctttcgt gcgatcttct aagaaacgct gagagaattt ttccagcgac tctggcaact
     8461 gataactgtt ggtctcttct tctgcccagg cgctgatctt gtcgatccat ttagcctgat
     8521 tgctacggtt aaacttgcgt cgatcaatac cagaagattc gatcagcgca tccagttcac
     8581 ccaccgcgtc gcgccactgc tgttttaccg tatcaatacg cgccacaatt tgcgcgtggc
     8641 gggaagccag cgtttcatca tcgggcggcg gcgctttgat aaccggcgct tcgccttgca
     8701 gataacgatt aatatcgcgc agtaacgcct gcggcccttt ccaggtttca aagacgacct
     8761 gggcaatttc acgcggcagc gggtagcagt ggcgacgcca gaaatcggcg caggcctggt
     8821 aacgtagcag agactcatct tcaatcagct gctgctcaaa cagcatgccg gattcaaagg
     8881 cattcaggtt gagcatgcgc tggcaaaaac cgtgaatagt aaagactgcc gcttcatcca
     8941 tctgccgttc ggctaacaac aaccactgcg cggcttgcgc tttatcgtcg atctcttcca
     9001 gcaggcgttc gtacagtgga ttgtcggtgg tttcacgcag acaggcgatg cgcaactcgt
     9061 ggatattgct acggatacga ccgcgcaatt ctgccgtggc agcctcggta aaggtgacca
     9121 ccagcagttc ttcaacggtc agcgggcggg gaaaggcggc agaaccgcct agtccaagta
     9181 acaggcgcaa atagagcgcc gcaatcgtaa aggttttgcc tgtgccggca gaggcttcaa
     9241 tcaggcgctc gccctgtaag ggcaagcgca aaggatctag tgtctcggcg acatcactca
     9301 ttcttttcac tcatcagggg cattgtttgc tgcaacgcgc tgacgttctc ccacactttc
     9361 cagccttcag gatgcacata ttcggctttc ccgttctggc tgccggaaat ctgcgacaga
     9421 atagccatgc cttgcggctc gaccaccgcc tgatggaaga aatcagcaag tttttgcggc
     9481 gtcagcagtt ttatctgggc cacgatttta tcacgcgaat cgaagcgcat attgccgcga
     9541 tcgaaatctt tacttaactt cgatgcttct tcgccgagcg tttgcggtgc ctgcagcatc
     9601 tgggttatta ccgcctgctg gatttgcgca aactcctctg gcttcatcgc ccgcaatttt
     9661 gcctctgcgg ttgggaaaaa cgccttgtaa cgctcccaca agaatgaagg ctgtttatca
     9721 ttgctttgca aaaggaagcc catgccccac tgacgcccca cgctcattgg aaacgcaaac
     9781 acggcatagc ccaactgttc ttcggtacgc aactgattgt agaaccacgg ctgtacgatc
     9841 tgccccaaca gagagctata ggctgagctg gtgtattcat cgtagccagt cggtacaaat
     9901 accgctgcca gtgcggagtc ggtgctgtta ccggcttttt caaagatgac agattgtttt
     9961 ttatcgacca cgacatcttt gttacggcac cactccgaac catcagcgcc caactgtttt
    10021 tgcacatcgc gtgccagcgt tgttgcctgg gcttcggtca tgttgccaat aaccataaac
    10081 tctggtcgag cccctgattt taaggcgtcg cgataggcca gcacctcttt caaagtgatt
    10141 gaaggcaaaa ttttgcgccg ttcatctcgc gagaagtacg gcacttgcga gagcatctgc
    10201 gcgggcataa tcgcctgctc gaacgctttg cccttttctg cggaatccat catctggtta
    10261 taccaggact tcgcctgctc aagctgatct tccgtagcgg tatagctaaa gtacccctcg
    10321 agcaatgcct ggaacagctg cggcagacgc tgggtgtaac cattagcatt aaccataagg
    10381 ccgttgttag cgttggtgga aaaactaatg ccaccaaccg acgcctggtt gcttaactga
    10441 tcaagcgcta gcccggcaag ataatcattg agcgcaaaca tcacctgatt gcgggcgctg
    10501 tccatggctt tcggattacg caaaatcagg ctgacatcag ctttgggttc gctggaaaaa
    10561 taacggcttg gcgcatagac cacacgcaga ttcgactcat caacaataag ctctggatgg
    10621 tcatatttct tctctgactt aatcagcgag aaatcgtcag gaatataggg gttaagctct
    10681 ggcaaagaga gcgcaatgtc ggcggctttt ttctgccagt cagcgaaagt ttgtgcgcta
    10741 attttatcga cctgatacgg cgcatcgaca aagtaagccg ttttgttgtg cggctctttc
    10801 gggctgatat accagatacg cgcattctgc ggcgtcatca tcgccaggcg ttcttttact
    10861 gctttagcgt cgtaccgatc ggcaatattg actgcatcca gcgtatgctc aacaggaacg
    10921 cgaatcatgg tatctgccag ccattcgacg taatccatat cacgggtgat tgacggataa
    10981 cgaaagtcga tatccagcac attcgccagt tcatcgaagt attgtttatc aatccctttt
    11041 tcgcgtaaca gattgaggta gctaaaaatg gccgccacaa cctgatcgcg attcgccaga
    11101 cctttatcgg ttaaagacgc agagatcgct aatacgccgc tgttgccgtt gacgatagga
    11161 tcggaattgg cgctaatgcc ctcaactaat ccctgctttt gcagccagtc agaaagtgta
    11221 cctgggctgc gattaccgat cagataggta atcaattcat ccgttttact acggaacttc
    11281 gctgaattgt tatcgatgcg aaactcaacg cgcagaactt tacgcggcag cgccgggacg
    11341 taatgaatga taataccttt ttgcgcgtcg gtgactaccg gcacggtgat ttccggtttt
    11401 ttgctctctt tgttcggcac gcgaccaaag gtgtccgccg ccatttttgc caactccggc
    11461 agcggtttat tgctgtaaat aaccgccttc atcagattgg cagagtaata tttctcgtga
    11521 aaatctttca gcgcctgctg caccggattt ccgggtttgt cgcttaaagt ttcgaggtta
    11581 ccaccagaaa actttgaacc ggggtgtgcc gggttaatgg tttctgcgct gacctgtgcc
    11641 atgcgcatcc cgtcacgcgt acgcgccatg gttaattcgg cattcactgc attacgttca
    11701 cgttcggcgt atttcttgtc gagcaagggt tctgcaatag cgtccgccag gcggtctacc
    11761 gcaccgggca aggcgtcgtt ctcaacttcc agatagaaag ccgtgcgata cggtgccgtg
    11821 ctggcattgt gactgccgcc gtgcattttg agatattcgg ccagactgtc agcctgcggg
    11881 tactttttcg accccatcag actcatatgt tcaaggtaat gtgccagccc ctggtacgcc
    11941 tcgggatctt ccagcgaccc aacgggcact accagcgccg agagcgattt aaccgcctgc
    12001 ggatcagaaa ccagcaagac caccataccg ttatccagac gtatagcctg atactggcgg
    12061 ttatctttat cacttttacg gatggtttcc tgaatcggct gccatcccgt ttctgcctga
    12121 cttaagggtg cccaaagggc aacaaacaac aataatgctt tgaaccaggt gctgcggggc
    12181 attcacggac ctcataatca actttatttt ctggccagat tcaacaggtt aacgcaaatc
    12241 atctgccaga atttaatctt gtgctgaacg agtcagccta tgtctatata tccatcagtc
    12301 cgtgactggt gcgcatcata aagcaagcag atatattgcg caatttttat acagcactca
    12361 tgactgatta aagcgaaaca gcggtaacag gaaacgttgc gactgttcaa cgatagcctc
    12421 cattgtctct ggtgttaatt gccgccagag ccgttgatac cagatatcat caccttcgcc
    12481 acgcaccatc atgttgcctt cgtaagcctg aaggaatttc gtacgggctt tttgtaacgt
    12541 ggaatcgtca tccagcatgg catcgttttg cgcgtcataa caggttttta gccacgcgcc
    12601 gccactttca ggtaacacca gcaatggcgc ggacattcct tcacgatacc cctcaatcag
    12661 ttgtgagagg taatgcaaag cctgttcggc tgcaagcggc ggaaaacgcc actcgccgtc
    12721 tttgcgtaga aaaaggcgac tttcaccatt accaccgctg gcacagtaga caaggtgttc
    12781 cagccaaagt tgcattccct gcgccacact taataaagag ggacgccagc gcaacaggcc
    12841 atccggctgc acctgcggca accagccagt tatctgcaca ccgttgcagg cgagatcaat
    12901 ttccatactc tgccccagct ggcgacaggc aatgactctg tcggcaagct gctgcatctc
    12961 ctggcactgt gtttcccaga aaatttcacc aaaagcgcca tacggtaaat cccccgccgc
    13021 ccggaagcgg cggaacaagc gttcggcatc atcctgctca accagtgcat tcaataactg
    13081 ctgattgatt tgataacggc taagcccttc cagaataaat ggctcggtgt cggggatttc
    13141 gctgtcttca gtacggaagt tcacctgcaa acgcatctgg aagaacgccc gtaccggatg
    13201 tgcccagaat cgttgtagcg tttccagcgg cacggtttcc ggtaaggtaa acggcagcgg
    13261 ctgaacaaat tcagaatgtg ctttaccagc ctggctggcc gcaggtagcc attcacgagc
    13321 atagctttgt cgttcgcctg gctggtagtt ttgcggatca aacggcatcc gggtatggag
    13381 gcaagtaaga tgcgctttta cccttgcctc gctttcatca cagttgagcg cttcatcgcc
    13441 cggtagataa tggctttgcc cgatgtagtc gatcagttcc tgcaccagta ccgacgggaa
    13501 acgttcactg ttatcctgaa tagaacgccc gatatagctg atatagagtt tttgctgcgc
    13561 ggaaattaac gcttccagga acagatagcg gtcgtcatcg cgacggctac ggtcgccacg
    13621 cttcggtttc tggctcatca ggtcaaagcc caatggcgca agctggcgtg gataaacgcc
    13681 gtcgttcatt cccagcaggc aaaccacttt gaatgggatg gaacgcattg gcatcagagt
    13741 acaaatgtta accggtccgg cgagaaaacg ctggctgata cgttcctgat ccaggcgctg
    13801 tgccagttca tcacgcaata gtgacagcgg caccgcgtcg ccatactgcg cacctaaacc
    13861 ttcggcgata atcgcctgcc attgttgttc gatcagcgtc atcgccgctt cggtttccgt
    13921 atccggcagg aagaaggcgt tgagcatatc gcgacaaacc ggcaaccact cttccagcgg
    13981 acgctcctgg gccagcccgc gacgccagat gtttagctgc attagcagtg aagccagatg
    14041 ccccaccagt tctgcaatta agccgctcga ttcatcataa ggtagaaccg attgccactc
    14101 gccctgcgcg ctctccatcg cgtagcccag caacatacgc gtcaggccaa atcgccaggt
    14161 gtgttgtccg gtggcgggga gttccagctc gcgaacgttg tcgtcatcta tcccccaacg
    14221 aatgccggat tcgttgaccc actggcgtaa ataacgcagc ccttcttcgg tgatgtcaaa
    14281 ccgcgctgcc agcaccggca catccagtaa tgccagcacg tcttccgaca caaagcggct
    14341 gtctggcagc gataacaggc tgataaacgc ttcaagtaca ggatgcgact gccgcgcccg
    14401 acggtcggaa atggcgtaag gcaggtaacg atccgcaggt gcgctaccaa acacagcctg
    14461 aataaacgga ctgtagctgt cgatatcagc caccatcacg atgatgtcgc gcggagtcag
    14521 tgtcgggtct tcctccagca tcgccagcag gcgatcgtgt aaaacttcaa cttcacgctg
    14581 cgggctatgg caaacgtgga aggtgatact gctatccagt ggatcaagcg ggcgtttgtt
    14641 atcgctacgg gaaaactctt cgatgttcac accagcaacg gcgcggtttt ccagttccag
    14701 aatgtcagac tgaatattat gcagcaggtt atctggcgtc acatcgacaa aagcgtccag
    14761 ctcctggctg ctctccaggt cagaaaggag ataaatgtag tcgcgcccaa gcttgcccca
    14821 tgaagccagc agcgggttgc cgacatcctg ttcaccatcg ctgttaaaga gctgcccggc
    14881 attttcgctg tcgcgaaata gcggtaattc gcgatcttca aaactgtgtc ggcgctggcg
    14941 agtcagtagt ttcgccagat aagctggatc tttaatgtcg ccccagtaat aacggcaggg
    15001 gttggtaaac aggagatgga tttcaatatg tttacccagc gcctgtagcg cctggagata
    15061 aacaggcggt aacgcggaaa taccgcatat aaagacgcgc gaaggtaacc ccggcgggca
    15121 ggtcgtcgcg gactccagcg tttcgataaa gcgctgatag agattggcgc ggtgccagcg
    15181 cggttgcccg agttgatggg tatattccac caaagccttc cacaacggcg cttgccaggc
    15241 ctgtgcttct cccagccctt caaccaaatg tcctgtttcc cactgtgcca gccagtccgg
    15301 acgatagacc agatactggt caaacaggtc cgccgctttt gaagaaagct ggaacagttt
    15361 tcgcttgtcg ctatcgtcag tcagataatg ccgcaacagg gtaaagtctt cgcgctccaa
    15421 cagttgcggc agcagagtca tcagtttcca gctcatgctc tgtttgttaa aggcgctctc
    15481 tttggggatt tccggtaaca cccggacgaa catatcccag ataaagctcg ctggcagcgg
    15541 aaaatcaatg tttgccgcaa taccaaactt ttgcgacagg gtcatttgca gccactgcgc
    15601 cataccggta ctttgcacca gaatcatttc tggttcgaaa ggatcgtcca gccgttcgcg
    15661 ttcgacgata aactccatca acgcttccag cacgtccaga cgattggaat ggtagaccct
    15721 taacatagcg gctcctgact actgacgatt cgggcaatgc aggcgggtca tctcgccttc
    15781 tctgcccccg ggtgaaacta gcgtaacgct gatgctgaca catcccgcct gcgatgtctg
    15841 cattcggttg acctgccagt gggcaggtgg cgaattcgcg cgcagttgcg tttgctgcca
    15901 gccatgccgc cagagctgtt ggtactggtt tctactggca agactgttca ttaatgttcg
    15961 ctggaaaccc gataacgcag tgacaatcat caccatcagc accattgcca acatcacctc
    16021 cgggaggcta aagccttgtt gattcttcaa ggaagctgac ataacgcccc ctctttcagc
    16081 ggacaaaaat cgctccagcc gcgtggcgaa aaaacaatgt ttccatcgat gacttcgcct
    16141 gttcgccaca acgaaacgcc ttcataaccg gcaatcaata aggcctcatt atctgccaat
    16201 aaacgcagac aaacccgggc accggtggca gcgtacagta agcactgaac tgcgggctgt
    16261 gtctgccagg agtgcatttt tccccactcc agcgtcgact gaacgatggc ctggcggcgc
    16321 aatgactgac tttccatgct cacgcgagag gcaaaactgc gatcctgctg actcattcct
    16381 tgtaatagca agctacccaa aaccagcagc atcaggacca gagccagtga cgaaacaccc
    16441 ttttcgcggt tcacaggttg aatcctgtca cgctatagct ggcatccacc acggtttgcg
    16501 gttcagactt actggcagca cgcatattaa ccgtcaacac cggcgagaag ccgctgacat
    16561 cctgacgtac gacctgaaaa gtgtcgataa tgatggcatc cggattagtg actttatccc
    16621 agcccttacc ttcacaggat gtcgcaccgc gtagcgtttc cagcacatgc tccttcagac
    16681 gaaatccaat ctgatcggac tcttttaccg gttcgcgatc ccagataccg ttactgttcg
    16741 catcccactg cacaatgata cagtcaccct gtccgacaat ttccagccct tcgccggtac
    16801 agctgccatg acaataaccc gccctctgga gatgcttcgc gacggtaaat acccgcagcc
    16861 agatttcatc ttccagcgcc agcttacggg tgctcgttaa actttcacgc tgtaacgcag
    16921 gcagaaagcg tgcagccccc agcaacaata cgctactgat cgccatagca atcaacactt
    16981 ccagcagaga aaaaccttgc tcttttacag gcatccttct gtttctcctt gctgacaaag
    17041 ccggagtctt ccccacggcg aaaccaccag ccaccactcg cccgttgagt ttttgaagcg
    17101 aatatgcccg gcccatgcgg tattgcgcag gccaaagaaa gcaagcgaag gtgtcaggtc
    17161 gctcatttcg acttctggcc agcgcggcac aaagaccaat ggtgaactgc catgacaggt
    17221 attcgcccca gcaacggaac tcacaaggca ccataacgtc ccctccctga taagactgat
    17281 actgtggtcg cggttatgcc agttggcatc ttcacgtaaa tagagcaaat agtcccgcgc
    17341 ctggctggcg gtttgccaaa gccgttgcga ctgctgccag tattgccagc catagagccc
    17401 acttgcgctt agcatgacca aaatcagcat cgcgaccagc gtttcaatca gcgtataacc
    17461 acgttgtgtt ttcatgccgg cagtatggag cgaggagaaa aaaagacgag ggccagtttc
    17521 tatttcttcg gcgcatcttc cggactattt acgccgttgc aggacgttgc aaaatttcgg
    17581 gaaggtgtct cgaagaattt aacggagggc aaaaaaaacc gacgcacact ggcgtcggct
    17641 ctggcaggat gtttcgtaat tagatagcca ccggcgcttt aatgcccgga tgtggatcgt
    17701 atccttcaat ctcaaagtct tcgaaacggt agtcgaagat ggattcgggt ttacgtttga
    17761 taatcaactt cggcagcgga cgcggttcgc ggcttaattg cagatgagtt tgatccatat
    17821 ggttgctgta caggtgcgtg tcgccaccgg tccagacaaa atcacccact tccagatcgc
    17881 actgctgcgc catcatatgc accagtaacg cgtagctggc aatgttaaac ggcaggccga
    17941 ggaagacgtc acaggagcgc tgataaagct gacaagagag tttgccgtct gccacataga
    18001 actggaagaa tgcatggcac ggtgccagcg ccattttatc cagttcgcct acgttccacg
    18061 ctgaaacaat aatgcggcgg gaatccgggt cgtttttcag ctggttcagt accgtagtga
    18121 tctggtcaat atgacgacca tccggcgttg gccaggcgcg ccactgttta ccatacactg
    18181 gcccgaggtc gccgttttca tcggcccatt cgtcccagat ggtgacattg ttttcgtgta
    18241 gataagcaat gttagtgtcg ccctgcagga accacagcag ttcgtggatg atggaacgca
    18301 ggtggcaacg tttagttgtc accagcggga atccatcctg caggttaaaa cgcatctgat
    18361 gaccaaaaat ggaaagcgtt ccggttccgg tacggtcgtt tttctgtgtg ccttcgtcga
    18421 gcactttttg catcagttct aaatactgtt tcatggttcc tcaggaaacg tgttgctgtg
    18481 ggctgcgacg atatgcccag accatcatga tcacacccgc gacaatcatc gggatggaaa
    18541 gaatttgccc catgctgatg tactgcaccc aggcaccagt aaactgcgcg tcgggctggc
    18601 ggaaaaactc aacaatgatg cgaaacgcgc cgtaaccaat caggaacaag cctgagacag
    18661 ctcccattgg gcgcggttta cgaatataca ggttgaggat aataaacagc accacacctt
    18721 ccagcagcag ctcgtaaagc tgtgatgggt ggcgcggcag cacaccgtaa gtgtcgaaaa
    18781 tggattgcca ctgcgggttg gtttgcagca gcaaaatatc ttctgtacgg gagccaggga
    18841 acagcatggc aaacgggaag ttcgggtcaa cgcggcccca caattcaccg ttaataaagt
    18901 tgcccagacg cccggcacca agaccaaacg gaatgagtgg ggcaataaaa tcagagacct
    18961 ggaagaagga acgtttagta cggcgggcga agataatcat caccacgata acgccaatca
    19021 ggccgccgtg gaaggacatg ccgccgtccc agacacggaa aagatacagc ggatcggcca
    19081 taaactgcgg gaaattgtag aacagaacat aaccaatacg tccaccgagg aagacgccga
    19141 ggaagcccgc atagagtaag ttttcaactt catttttggt ccagccactg cccggacgat
    19201 tcgcccgtcg tgttgccagc cacattgcaa aaatgaaacc caccagatac atcaggccgt
    19261 accagtgaag cgccacgggt cctattgaga aaatgaccgg atcaaactcc ggaaaatgca
    19321 gatagctact ggtcatctgt caccacaagt tcttgttatt tcgctgaaag agaacagcga
    19381 ttgaaatgcg cgccgcaggt ttcaggcgct ccaaaggtgc gaataatagc acaaggggac
    19441 ctggctggtt gccggatacc gttaaaagat atgtatatga tccgcgctat aaccccccgc
    19501 gaatcagccc gcccatgccg cgacgctcca taaaggcagc aacctgatgg cgaacttcgg
    19561 tcgccagttg cgcttccaga ctacgctgcg caagattttc tgcttcggca aaatcaatgc
    19621 gccgcagcag gtattttacg cgcgctacag aacgtccgtt catagacaaa tggcgatacc
    19681 caagcccaat gaggattgcc acgcacatcg gatcgcccgc catttcaccg cacaaacgga
    19741 gatcgattcc atgtatttcc gcttcccggg cgatcatcgc cagagctcgt aacattgcag
    19801 gatgaagact gtcataaatg ttcgcaaccc gggtattgtt gcgatcaacg gcaaggatgt
    19861 attgcgtcag atcgttggtg ccaacagaga tgaaatcgac ccgctttgcc agatgcggca
    19921 gcataaatac cattgacggc acttccagca tgatgccgat acgtggtttg ggaatttcgt
    19981 aaccgatcat ctcctcgact tcacgtccgg cacgttcaat caggcggcgt gcttcgtcaa
    20041 cttcatcgag gctggtgacc atcggcaaca gaatattcag gttgcccgta gcggcattag
    20101 cacgcagcat cgcccgcacc tggatcaaga agatctccgg ctgatcgagc gtaatgcgaa
    20161 tcccacgcca acccaggcat ggattctctt cgctgatcgg catgtaaggc agctgcttat
    20221 ctgctccgac atccagcgta cgcaaggtga cgggtttatc attaaacatt tgcagcatcc
    20281 cctgatactg cgccacctgt tcttcttccg acggaaaacc actttgcagc atgaatggga
    20341 tttcagtgcg ataaagtcct atgccatcaa tacggctgcc cagtttttct tcatgttccg
    20401 ggcttaaacc ggcattgagc atgactttaa tgcgttcgcc gctttttaac tgggcgggta
    20461 aattgacgtc atcttccgcc agacggctaa gctcgatctc ttcactaatt agccgctgat
    20521 attcttgcag cagtaccggc tccggatcga ccagcaattc accgcgataa ccatcgacga
    20581 tcagcgtccg acgatgcagt accgaaggct gaatatccgc gcccatcacg gtaggtatcc
    20641 ccagcgcacg taccatgatc gcagcatggg agttggcagc gccatcgcgc acgacaacac
    20701 cgactaagcg atcctggggc agctcagcaa gcgttgtcgc tgacagttca tctgccacca
    20761 gaatgaaacg ttccggccag gcgttcggcc cttgattagc gtcatcaaga tgaaacagca
    20821 atcgctggcc cagcgcacgt aaatcgccag cccgctcttt gagatagtta tcgcttagcg
    20881 cggcaaactg ttcggcaaat ttttcaatga ccgtttttac cgcccactct gccaccgagc
    20941 ctttatcaac ctcggcaaac aattcgcgcc gcagccgggt atccgaaagc aggtgtgagt
    21001 aaagatcgaa aatagccgcc gtttcttttt gtgcaccggc ggcaaagcgt ttgctgtagc
    21061 ggcgaaactc gtttgccgct tcttccagcg ccccggtcag tcgttcgcgt tccagagccg
    21121 gatccaacgt tgatgcctga tacacctgtt ccattaaagg taacgtggca tcctgccagc
    21181 cttcggcaat cgctacacca ggcgctgccg gtaatgcacg gattcgcgtc tggcgatatt
    21241 gcccaaacaa ggcagtcaac tgcgactgag aaagaatcgc tgccatctgg gtggcaagcg
    21301 tcaccaggaa ggattcttca ctttcgtcat actggcgcaa ctctcgttgc tgtaccacca
    21361 gtacaccaag caactggcga cgttgaataa ttggtacgcc taaaaacgcg cggaaacgtt
    21421 cttcttttac ggaggggatg tatttgaagc tggggtgctt ttgcgcatct gcaaggttta
    21481 tcggttccgc cagcctgcca accaggccga cgatcccttc atcaaacgcg agcgttacag
    21541 tgcgaccacg tggttttttc agcccccggg tcgccatcag gtagtaacaa cgtcgatcat
    21601 gatcggccag gtagaccgaa cagacctcgg tatccatcgc aagacagatg tcggtaacca
    21661 gaatatttaa cgcctcattc aggcgtggtg cgctggctac cttttcgact atttcgcgca
    21721 ggcgagtgag cataattggc gtgacttaac ctcttttacg tcgataagca gatgcgtttt
    21781 gtggttttgg cgtattttcc tgcagtgaca tcaccacact cgcgaactct ttcattaccc
    21841 tacggtagac atcacgttta aatgacacca cctgtctgac tggataccag taacttaccc
    21901 atcgccagcc gtcaaactct ggtgtactgc tggtttgcat attgatttct gcatcgccgc
    21961 tcaccagctg caagagaaac catttttgtt tttggccgat acaaaccggc ttcgtgtccc
    22021 aacgcaccaa acgtttcggt aatttgtagc gcaaccagtt acgcgttgaa gcaaggattc
    22081 gaacgtcttt gcggcttaat cctacttctt caaacaattc acggtacatc gcctgctctg
    22141 cggattctcc ggggttgatt ccgccttgcg gaaattgcca ggagtgctga ccaaatcgcc
    22201 gggcccacat tacctgcccc tggcgattac aaatcacgat acctacgttt gggcggtagc
    22261 catcgtcatc aatcaccgga ctacctcaaa ataaagcttt atatacgaat gattgtttca
    22321 tactccagga agacggtaaa ccactctctg cagggcatta cacactaata acaattgaat
    22381 aactcacagt tatgtgcaga gttataaaca gaggaagggg tggatagccg tttttattca
    22441 ccttttctgt ggatagagtt gtgaagaact acggaattac tacgggaaaa cccggagcat
    22501 tctgaataag cccgatatag acattttaaa atattcatta tcagtcaatg cattacatgt
    22561 aatttgagta ataaaaaaga gtagttcgtg acccaggtca cacctctcat ttacgggttg
    22621 gcgaaagatc aaccaatgcc gtatttatcc acagaatgtg ccactaagtt aagcactgaa
    22681 ccactaaaaa ctggagtttc gtcgcacgtc aaggctgtaa atggaaacag tagtgaaggt
    22741 ttttcacagt tatcccagct ttctgtggat aacatggtgt aagatcctgt ttattttcag
    22801 tgaccagatt tggaaaaccc gttgcagtgt tgcgcaactc gattaccggc aacctaaaaa
    22861 gcaatataaa tcagagaatt gaacaacgca tgtggaaaaa gttacactgc gaatattcgg
    22921 cacttaattg ctgtttgttt tttaaccaag gtatcatgac atgtcccaac ctcgcccact
    22981 gctctctcct cccgaaactg aagaacaatt gttagcgcaa gcacaacaac tttctggtta
    23041 tacattggga gaactggcgg cacttgccgg gctggttacg ccggagaatt taaaacgcga
    23101 taagggctgg attggcgtgt tactggagat ctggctaggt gccagcgcag gaagtaaacc
    23161 tgagcaagat tttgctgccc tgggcgtgga acttaaaact atccctgtgg atagtcttgg
    23221 tcgtccgctg gaaacaacat tcgtttgtgt tgccccgtta acgggtaata gcggggtgac
    23281 ctgggaaacc agccacgtac gccacaagct caaacgcgta ctgtggatac cggttgaagg
    23341 cgagcgcagc atcccgctgg cgcagcgtcg cgtaggatca ccgttactgt ggagcccgaa
    23401 tgaagaggaa gaccggcaac tgcgcgaaga ctgggaagaa ttaatggata tgattgttct
    23461 cggtcaggtt gagcggatca ccgctcgtca cggggaatat ttgcagatac gaccgaaagc
    23521 agcgaatgcg aaagcgctta ccgaagccat tggtgtccgg ggcgaacgga ttctgacgct
    23581 gccgcgcggc ttttatttga agaagaattt caccagtgcg ctactggccc gtcattttct
    23641 gatccagtag ccatcgcttt gacctgccgc tttccgggca tataattacc gcttcatttt
    23701 tttggcaggg ctttttagat gttatttgca tggataaccg atcctaacgc ctggcttgcg
    23761 ctcggtacgc tgacgctgct ggagatcgtt cttgggatcg acaatattat ttttctttct
    23821 ctggtggtgg caaagctccc cacagcacaa cgtgctcatg cgcggcgtct ggggttggcg
    23881 ggagccatgg ttatgcgtct ggcgctgctg gcatcaatcg cctgggttac gcgcctgaca
    23941 aatccgcttt ttacaatatt cagtcaggaa atttccgccc gtgatttgat cctgcttctg
    24001 ggtggcttgt tccttatctg gaaagccagc aaggaaatcc acgaatccat cgaaggtgaa
    24061 gaagaagggc tgaaaacacg cgtttcatca ttcctcggcg ctatcgtgca gattatgctg
    24121 ctggatatta tcttcagcct cgactcggtg attactgctg tgggtctgtc agatcacctg
    24181 tttattatga tggctgccgt ggtgattgcc gtaggcgtga tgatgttcgc cgcgcgctcg
    24241 attggtgatt ttgtcgagcg ccatccttcg gtaaaaatgc tggcgctctc tttcctgatt
    24301 ctggtgggct ttaccctgat tctggaaagt ttcgacatcc acgtaccgaa aggttacatc
    24361 tacttcgcga tgttcttctc tattgcggtt gaaagcctca acctgattcg caacaaaaag
    24421 aatccgctct gatactccgt acgctctcct gcgggagggc gtactcactc ctgttaccct
    24481 cacagtttaa gattttcctg ctttcaagat atataacgtc ggtttataaa cagactatta
    24541 tcataggtgg gccaaatgag gataaccgga tgaaaaaatg ggcagtaata atttctgcag
    24601 tcggactggc gtttgctgtt tccgggtgtt ccagtgatta cgtcatggcg accaaagatg
    24661 gccgtatgat tttgaccgat ggaaaacctg aaattgatga tgataccggg ctggtgagtt
    24721 atcacgatca gcaaggtaac gcgatgcaaa ttaaccgtga tgatgtttcg caaattattg
    24781 aacgttaaca aataaggtca gcatccggct ggccttaaga tttttctctt tcccttttcc
    24841 cttccctctg ccatttttat attccttatg tcgtgattat aaaaaggaaa cggctatgca
    24901 atatcaccgt ataccccaca gttcgctgga agtcagcacg ctggggcttg gcacgatgac
    24961 gtttggtgaa cagaacagcg aagccgacgc ccacgcacaa ctcgactatg ccgtcgctca
    25021 gggcattaac cttatcgacg ttgccgaaat gtacccagta cctccgcgcc ccgaaactca
    25081 agggctaacc gaaacctacg tcggcaactg gctggcgaaa catggcagcc gcgaaaagtt
    25141 aattatcgcc tccaaagtga gcggaccgtc gcgcaataat gacaaaggca ttcgcccgga
    25201 tcaggcgctg gatcggaaga atatccgtga agcgctgcat gacagcctca agcgtctgca
    25261 gactgattac ctcgatctct atcaggtgca ctggccgcag cgcccaacca actgcttcgg
    25321 caaactcggt tatagctgga cggattctgc gcctgcggtt tcgctgctgg atacgctgga
    25381 cgcactgaca gagtaccaac gcgcgggaaa aattcgttat atcggcgtgt cgaacgaaac
    25441 tgcatttggc gtaatgcgct acctgcatct ggcggacaaa cacgatctgc cgcgtattgt
    25501 caccattcag aacccttaca gtctgttaaa ccgcagtttt gaagtaggtc tggcagaagt
    25561 cagccagtat gaaggggtcg aactgctggc ctattcgtgc ctgggtttcg gcacgctgac
    25621 cgggaaatat ctcaacggtg caaaacccgc tggcgcacgt aatacactct ttagtcggtt
    25681 cacccgctat agcggtgagc aaacgcaaaa agccgtcgcg gcgtatgttg atatagccag
    25741 acgtcatggc ctggatcctg cacagatggc gctcgctttt gtacgccgtc aaccgtttgt
    25801 tgccagcact ctgctgggcg caaccacgat ggagcagctg aaaactaacg tcgaaagttt
    25861 gcatctggag ttaagcgaag acgtattagc tgaaattgaa gcggtacatc aggtttatac
    25921 ttatccggca ccataagatt aacgccggtt aaaaccggcg ttaaatatta atgacggcgc
    25981 tgccagatcc acagcgccgt tattgccagc gcaaatagcg caccgaagcc aatgccaatg
    26041 ggcacgaccg ggatgcctac cattaccgcc agcgagtaaa tgcccagcat caacaacatg
    26101 gcgctgtttt cgccaaggtt ttgtactgca attgcattcc ccgccccgac gcttttttta
    26161 ccccgttcct gtagcaacgc attgagcgga acgacaaaaa agccccccag cacgccaatc
    26221 agcatcaaca aggcataggc tgacagcagc tcgtgttgca gggagaaaat cagtaccacc
    26281 acgccaatca aaatcccggc tggcatacag cgtgacacgg tttccagcgt aactaacttc
    26341 gctgccgcac cagcgccaac cacgatacca atcgctacca tcgcgttgag ataggtcggc
    26401 gtagcgttat cggtaatgcc cagcgccacc ggtacccaca gcaccaacag gaaacgcagc
    26461 gtgacacccg ctccccagaa taaactggtg cccaccagcg aaaaacgcgt ttcaccattg
    26521 cgccatagcg aggtgcaggc attcaggaaa ctgcgggtca tgctgatgag attccaggac
    26581 tgccccggac gcgccgccgc cagtttagga atgtagatat tggcaacgac cgcaccaccg
    26641 taggccagtg cgcatgcggc cagggcgacc aggacatgcc agtcagccag tacaccaccg
    26701 gctacggaac cgagcaaaat cgccgctatg gtagaagctt ccattaaacc attagctttc
    26761 actaacttac tacccgtggt taattcgccg agaataccgt atttcgccgg tgaataggct
    26821 gcagcaccaa cacccaccag cgtatagccg agaaacggat tgataccaaa gcagatactg
    26881 gctgcgccca gcagcttcag gccgttggca aacatcatca cccggccttt ggcgaagcta
    26941 tccgccacct gtccgacaaa cggcgcaaaa agaatgtaag cacctacaaa caccatttgc
    27001 aggatgggct ggctccactc cggataaaac tgcgctttca gtaacgccag agtggcaaac
    27061 agcagcgcat tatcgccaaa cgcagagaga aactgcgcca cgataaccgc tttcatcccc
    27121 ttcgaccaca acgaagtgtt agtgtgcact gactcactca tcgtgttgtt ccgcttcgtc
    27181 tacccagctt ttcaacgtga caaagtcagg tttgccgctg ccaagtaatg gcatctgttt
    27241 cagatagcga atatcgcgcg gtacagcaag ctccggcacg ccgtgctcgc gggcatactg
    27301 ttgcaactta tcgcgcgtca gttcgttatc tgtggtgaaa agcaccagcg cctcgccttt
    27361 gctggcatcg ctcttaatcg cagtggcatg gactttatct ggcgaaacac caagtgccag
    27421 ttgttccacc atttccagcg acaccatttc gcctgcaatt ttggcaaagc gttttgcgcg
    27481 gccctgaatc tgcacaaagc cctgctcgtc gaaacgcaca atatcgccag tgtcatacca
    27541 gccgcgctcc atttcgccgc gaacattctc ggcggtgggc acttccagta cacctggctt
    27601 ctccacccgc agatagccgt tcattatgtt cggccctttc agttgcaggc gtccgccctc
    27661 ttcgatacca ggaaccgaca acaggcgcgc atccattcct ggtagaatac gccctaccgt
    27721 accgggtttc gccgccatcg gtacgttgat ggaaacgaca ggcgcgcact cggtcacacc
    27781 gtagccttca aggatgcgca ggccaaattt atcctgccaa agctgtttgg tactttcttg
    27841 tagtttttct gcgcctgcca ccacatagcg tagacggtag aagtcaaacg ggttggcgaa
    27901 acgcgcgtag tgaccgagga aagtcgaggt accgaacaac acggtgcaac tgcggtcata
    27961 caccaactcc ggcacaatgc ggtaatgcag cgggcttgga taaaggaaca cctctgcacc
    28021 tgtgagcagt ggcgtaaaca ggcctaccgt cagcccaaag gagtgaaaca gcggtaacgc
    28081 cgacataaag cgatcgttgg tggtgaagtc ggcaatcgtt ttaatctgct cgacattcgc
    28141 cagaatgctt ttatggctat ggacgacgcc tttcggatgg ccttcagaac cggaggtaaa
    28201 aaggatcagc gcctcttctt ccggctgctg ttttacctgt gccagacgcg gcatcagcaa
    28261 atgagcgaag atccatactt tgtcggcagt ggtgacatct gcttttaaat cttccagata
    28321 gacccagcgc acctgagtaa gttgctccgg cagatgccag agtttgcctt tatcgagaaa
    28381 ctgacgggaa gtgaaaatgg ttttgatttc agccgccgta atagcactgg tcaacccttt
    28441 taccccggcg gtgtagttca tcattgcggg aatgcggcga cgggcgatgg ccccaaaaat
    28501 cactgccgca ctgatgcctg cattgggcag cattaagccg atgcgttcgc cttcaacact
    28561 gtatttttca aggatgcgtc caacaaacag cgttttcgta agcaatttgc gataggagtc
    28621 cggggtaaag ttaacgtctt cgacacattt cttcccggct ccgaagcggt acattgcgct
    28681 cagtaaagat tcgtacagcg tttcacgcgg gcgcaccgcc atacgcgctt ccatcattat
    28741 ttgatgcagc atttcgccag cgattttgcg acggtcacgg gcacgcggcg catccggcat
    28801 ctccacctgc gttggtggca aaatatgcag agtaatttgc gggaacaagc gacgtttaac
    28861 cagacctttc aggcggctga agtgcgtaag ttccgcccct tcaatacgca caggaataac
    28921 cgttgcacca gacttcgccg cgacaaatcc cgcgccatcg tagattttca tcagcgagcc
    28981 agtggtggtg atgcgtcctt cagggaaaat caccactggt cggccctgtt ccaccagacg
    29041 taccagatgt ttaatcgcca taggttgcgt cgggtcgaga ggaacaaagt cgataaatga
    29101 tttcagccaa cgcatatacc actgttggct tattgaggtg taaacggcaa ataccggacg
    29161 tacgggtaaa aacaatgcca gcagtatgcc atcaataaaa gagacgtgat taggcgtgat
    29221 tagaacgcgc tcgcccttca gtgccttggt gtcacccgta acgcgaacgc gatacaaaac
    29281 acggcacaaa tttcgaaaaa agctaaaaag cattccaact ccctttgctc tgattcagta
    29341 aaagcgaatg gagggagatt acacgagata aagaacgcga gcgacagtaa attaggtgcg
    29401 aaaaaaaacc tgcgcatccg cgcaggttgg tgcaagagac agggtacgaa gagcgtaccg
    29461 aataatctca ccaatcaata cctctgggat cttgattgtg gtctgcacaa cgactcttcg
    29521 ccagcgagaa aacgcaaagg aatgaaggga aatgcaacga ggtgtgtaaa ttgtcggtta
    29581 ctgttacaga ttgatgaccg gcaaaaaaaa acctgcgcat ctgcgcaggc tggtgtaatt
    29641 catgtgctca acccgaagtt gacttcacct atcaatacct ctgggatcac cactttagca
    29701 acctgaagcc aaacgccacc agcggtcaat cgcaacagcg tttcgcaaag tgtaaccaaa
    29761 ggtttgcaat ctcttttttt gtcttactta tttgcgtttg gctcacattc ccacaatgaa
    29821 aacacgccgc cccttgaacc aacgggcgtt ttccgtaaca ctgaaagaat gtaagcgttt
    29881 acccactaag gtattttcat ggcgaccata aaggatgtag cccgactggc aggcgtttca
    29941 gtcgccaccg tttcccgcgt cattaataat tcacccaaag ccagcgaagc ttcccggctt
    30001 gctgtgcata gtgcaatgga gtctcttagc tatcacccga acgccaacgc ccgtgcgctg
    30061 gcgcagcaga ccactgaaac gatcggtctg gtcgttggtg atgtttccga tccgtttttc
    30121 ggcgcaatgg tgaaagcggt cgaacaggtg gcttatcaca ctggtaattt tttattgatt
    30181 ggcaacggtt accacaacga acaaaaagag cgtcaggcca ttgagcaact gatccgccat
    30241 cgctgtgctg cgctggttgt ccatgccaaa atgatcccgg atgccgattt agcctcgtta
    30301 atgaaacaaa tgcccggtat ggtgctgatc aaccgtatcc tgcctggctt tgaaaaccgt
    30361 tgtattgctc tggacgatcg ttacggtgcc tggctggcaa cgcgtcattt aattcagcaa
    30421 ggtcataccc gcattggtta tctgtgctct aaccactcta tttctgacgc cgaagatcgt
    30481 ctgcaagggt attacgatgc ccttgctgaa agtggtattc cggccaatga ccggctggtg
    30541 acatttggcg aaccagacga aagcggcggc gaacaggcaa tgaccgagct tttgggacga
    30601 ggcagaaatt tcactgcggt agcctgttat aacgattcaa tggcggcggg cgcgatgggc
    30661 gtgctcaatg ataatggtat tgatgtaccg ggtgagattt cgttaattgg ctttgatgat
    30721 gtgctggtgt cacgctatgt gcgtccgcgc ctgaccaccg tgcgttaccc aatcgtgacg
    30781 atggcgacgc aggctgccga actggctttg gcgctggcgg ataatcgccc tctcccggaa
    30841 atcactaatg tctttagtcc gacgctggta cgtcgccatt cagtgtcaac tccgtcgctg
    30901 gaggcaagtc atcatgcaac cagcgactaa ccgcagttaa agcaattcca gcgccagtaa
    30961 ttcttcaatg gtctggcgac ggcgaatcaa ccgcgcctga ccattatcaa acagaacttc
    31021 tggtaacagc ggacggctgt tgtagttgga tgacattgat gcgccatatg cccccgtatc
    31081 atgcagtacc agataatcac ccgccttcac ttccggcaag gcgcgggttt cgacatttcc
    31141 cccttcctgc tgggtaaaga catcgcccga ttcacataac ggcccggcga cgacggtttc
    31201 caccgttggt gcgtgttcca gagaacgacc atcagctgcc agggcactga tatggtggta
    31261 actaccgtac attgccgggc gcatcagatc gttgaacccg gcatcaacca gcacaaagtg
    31321 gcggctaccc atttgtttga cgctccgcac ttgagtaatt aatacgccag cctgcgctac
    31381 cagaaagcga cccggttcaa tttccagttt cacaggatgg cccaaatggc gggcgatttg
    31441 ctcacgcgcg gcattccaca gaccataata atgttcggta tcaaccgcct cttcaccctg
    31501 ttgataagga atagaaagcc caccgcccgc agaaatagcc tgtaaatcct gaccgaactc
    31561 gaggacctga cgcaccatag caccacatac ctgttccaga tgggcataat cgacgccaga
    31621 accaatgtgc atgtgaatgc cgaccagctg tagatgatga cgttgtatca cgtccagtgc
    31681 ggcgggcaga tcggtgtacc agataccgtg cttgctgttt tcgccaccgg tattggtttt
    31741 ttggctatgc ccgtgaccaa accccggatt aacacgcagc catacccgat gccctggcga
    31801 aacctgaccc agttggtcga gcatatcaac agaacccgca ttcaccggaa tttgcaattc
    31861 actgactcgt tcaagcgtcg cctgatcgat aacatctgcc gtaaaaacaa tatcatcggg
    31921 gtgcgtttgc ggattgtaac ccgccgccag tgcacgctct atttcgccta acgagacgga
    31981 atccactttt acgccctgct cacgcattaa gcgcaaaata tgaatattgg aacaggcttt
    32041 ctgtgcaaag cgcaccacat caaactgttt cagcgctgca atctgccgac gaataatttg
    32101 cgcatcgtag acccacaccg ggcagccaaa ttctgcgggc aaacgcagca gattttcggc
    32161 ggtgagatcg gtatcagtgc tgaacagtga atgtggcata acaaactcca gataagtgct
    32221 tttttatgat tacgccacat cataaaaaga ataaaaaata tcgatttatg tcgagtctat
    32281 gcaaaaatga tatggattac cggattgcga gagagcgcta atggccgccg ttaacttacg
    32341 tcatattgaa atttttcatg cggtaatgac cgccggaagc ctgactgagg cggcacacct
    32401 gctacacacc tcacagccaa ccgtcagccg cgaactggcg cgctttgaga aggtgatcgg
    32461 gctgaaattg tttgagcgca tacgtggacg attacatcct accgtgcaag gactgcgtct
    32521 gtttgaagaa gtgcaacgat cctggtacgg actggatcgc attgtcagtg ccgcagaaag
    32581 tctgcgcgag tttcgccagg gagaactgtc tattgcctgc ctgccggtct tttcgcaatc
    32641 ttttttaccg cagctcctgc aaccctttct ggcacgttat cccgatgtca gcttaaacat
    32701 cgtgccccag gaatcaccgc tacttgaaga gtggctctcg gcccagcgtc atgatttagg
    32761 actcactgaa acgctccata cgcctgcggg aacagaacgt accgaattac tctctttaga
    32821 tgaagtgtgt gtgttacctc cgggccatcc gctggcggta aaaaaggtat taacgccgga
    32881 tgattttcac ggtgagaact acatcagcct ttcccgtact gacagctatc gccagttgct
    32941 ggatcaattg tttactgaga atcaggttaa acgacgcatg atcgtagaaa cccacagcgc
    33001 cgcgtcagtc tgcgcaatgg tacgggcggg ggtaggcgtt tcggtggtta acccgctcac
    33061 cgcactggat tatgcggcaa gcggtttagt ggtgcggcgg ttcagcattg cggttccatt
    33121 caccgtcagc ctgatccgcc ccctgcaccg cccgtcatca gcgctggtgc aggcgtttag
    33181 tgagcattta caagcgggat taccgaaact ggtcacttct cttgacgcta ttttgtcgtc
    33241 agctacgaca gcataaaagc gacagcatcc tcggcatgga tcgtcgcggt atcaaacaca
    33301 ggcagaacac tgcgctcttc tggcaccagt aaaccaattt ctgtgcagcc aaaaatgacg
    33361 ccctgtgcgc cctgttctgc aaggcgagca atcacttgcg cataataagc gcgtgacgct
    33421 tcggtaaatt gccccagaca cagttcttca aaaataatct gattaatttt tgcccgttca
    33481 tccgcttcag gaataagaca gttgatggaa aattgttccg tcagccgccc gcgataaaaa
    33541 tcctgttcca tggtgtaacg tgtacccagc agcgccacac gagtcattcc tgccccggta
    33601 attgcacatc cagtggcatc cgctatgtgt aagaaaggca gagagcaacg agactcaatg
    33661 gcatccgcca ctttatgcat cgtattggtg cataacacaa taccttctgc gcccgcccgc
    33721 tgtaagccaa gcgccgcctc agccagaata tccccggttt tgtcccactc accgcgacgc
    33781 tggcactctt ctatttcatg aaaatcgacg ctatgtagca gcacttgtgc agagtgaagc
    33841 ccaccaagcc gctgtttaat gccttcattt atcagacgat agtaaggaat agtggattcc
    33901 cagctcattc ctcccagcaa accaattgtt ttcattcctc tctcctgatg tgtgttaccg
    33961 caacaaggct gaataaggaa cgagacaaac gcctcaacgg ccaggtgcct aatctctatt
    34021 aacgaaaaaa gggccggatg tacagcacat ccggcccgtg aaatcagacg ccgatatttc
    34081 tcaacttttc gcctgccatc agtttgcgct cgatatgttc cagcgtgaca tttttggttt
    34141 ccggaatgag ccagaaagtg atgcccacaa acgcaatgtt cagcgcagtg tagagccaga
    34201 acgtaccggc agcgccaatg ctatcaagca gtgtcaggaa ggtcgcgccg ataatcatat
    34261 tcgacaccca gtttgtcgtc gttgagcagg tgatgccaaa atcgcggcat ttcagcggct
    34321 gaatttcaga gcacaagatc cacaccactg gcgcggcgct catcgcataa ccggcaatac
    34381 acatcatcgt catgccaaca gagagccagg acaagccact ggaagccgta ccgttatcaa
    34441 actgcatcag gcagtaaccc agcaccagag tgcctaacgc catcacgctg aaaccaattt
    34501 tcagagccgg cttacgccct gctttatcta ccgtaaagac cgcaatgaag gtagcgaaca
    34561 taaaggtcag ccccacgacc agagtcgcaa tcatctgttg ttctgtggtc gtaaagcccg
    34621 ccattttgaa gatacgtggc gcgtagtaca tgatgatgtt cataccggta aactgctgca
    34681 tcgcctgcaa caacataccg agaaatacag cacgacggac gttacggttg atcttaaaca
    34741 gtgcccaacc gccctgtttt aacttcaggc tttcacgaat ttcgttgagt tcttctcgcg
    34801 ctttttccga cgtatcgcgc agcatacgca atacttcttc cgcctcaata tgacgcccct
    34861 tttccgccag ccagcgcggg ctatttggca ggaagaccac cagaataatc agcagaactg
    34921 ctggtaaagc aagaaccccc aacattgcgc gccagttacc gctataactg aacgctgtat
    34981 cggataaaaa cgccagcacg atgccgagag tgaccatcaa ctggtacatg ctgatcatct
    35041 taccgcgaac gttttcactt gccatttcag aaagatatag aggagcggtg taagacgcga
    35101 tcccgacagc aatgcccaac accacacgag cggcgattaa catctctacg ctggtcgcaa
    35161 aagcggaccc tatcgaaccg agtacaaaca ggatggcccc cgccatcagg ctgtatttac
    35221 gccccaggcg gaacgacagc caaccattaa acagcgcacc aattgctgcg ccgagcatca
    35281 tgctactgac cacccattcc tgcaaacgac tggtcagcac aaagtgatcg gtaatgaacg
    35341 gcaacgctcc ggcgattacg ccgatatcaa gaccaaataa caatcctgcg accgcagcag
    35401 ctaccgaaac aaacatattc atacgccgca tatcccgcag aggacgtggc gttaaagcag
    35461 attccgtatt gatagtaacc attttttcct gccagcagag agtaagacat agtgaaaaaa
    35521 tacgtgaaca attcacacag gtgtcaggtc ggaaatagca taaatatgga ttaaattgct
    35581 acgacatgtc gttatgtgat ggatattcca attttcaaat
//
LOCUS       DAPHCV010000047        34722 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.047, whole genome shotgun sequence.
ACCESSION   DAPHCV010000047 DAPHCV010000000
VERSION     DAPHCV010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.047"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            55..231
                     /locus_tag="RH033_002232"
     CDS             55..231
                     /locus_tag="RH033_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninF"
                     /protein_id="HDV2991411.1"
                     /translation="MLSPSQSLQYQKESVERALTCANCGQKLHVLEVHVCSDCCAELM
                     SDPNSSMYEEEDDE"
     gene            224..733
                     /locus_tag="RH033_002233"
     CDS             224..733
                     /locus_tag="RH033_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDV2991412.1"
                     /translation="MSDVKEKDIPGFEGIYKVTENGDIISCRKSKKLSHGIKPGGYAF
                     VGLYPGGGKRPSYKMVHRIVAEVFIDNPDGKPEVNHKDGNKLNNKVENLDWVTRTENA
                     KHGFDSGLLVHGFNHHFCKLTPEQVKSIYKSKGKYRDIAKEFGVCAQTVCNIKNKSAY
                     RRFLEGIDV"
     gene            998..1360
                     /locus_tag="RH033_002234"
     CDS             998..1360
                     /locus_tag="RH033_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDV2991413.1"
                     /translation="MNEYQFVLPYPPSVNTYWRRRGSQYYISDKGQKYRKDVQQIIRQ
                     LKLDIFTKSRLRIKVIADVPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRVIRG
                     VKVPGGRLGIKITELENA"
     gene            1357..1545
                     /locus_tag="RH033_002235"
     CDS             1357..1545
                     /locus_tag="RH033_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HDV2991414.1"
                     /translation="MNATIQTIPELLIQTRGNQTEVERMLSCARGTVLKYNRDSKGER
                     HVIVNGVLMVKQGKRGRP"
     gene            1542..2165
                     /locus_tag="RH033_002236"
     CDS             1542..2165
                     /locus_tag="RH033_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HDV2991415.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            2439..2513
                     /locus_tag="RH033_002237"
     tRNA            2439..2513
                     /locus_tag="RH033_002237"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2472..2474,aa:Asn,seq:gtt)
     gene            2519..2594
                     /locus_tag="RH033_002238"
     tRNA            2519..2594
                     /locus_tag="RH033_002238"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2552..2554,aa:Thr,seq:tgt)
     gene            2839..3042
                     /locus_tag="RH033_002239"
     CDS             2839..3042
                     /locus_tag="RH033_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDV2991416.1"
                     /translation="MYRMDKIREWFSYSFGGLTAMGGILSLNDWAVIIGILCTVGTFG
                     INWYYKRKEREDRLNGNVTGTTK"
     gene            3020..3517
                     /gene="rrrD"
                     /locus_tag="RH033_002240"
     CDS             3020..3517
                     /gene="rrrD"
                     /locus_tag="RH033_002240"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HDV2991417.1"
                     /translation="MSPALRNSVIAAISGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDINGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            3606..4043
                     /locus_tag="RH033_002241"
     CDS             3606..4043
                     /locus_tag="RH033_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HDV2991418.1"
                     /translation="MSRLTAIISALVICIVVCLSWAVNHYRDNAIAYKEQRDKAASII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASGM
                     GDGESPGLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            4248..4733
                     /locus_tag="RH033_002242"
     CDS             4248..4733
                     /locus_tag="RH033_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDV2991419.1"
                     /translation="MNNFVEITSRIGRMYQDFLISGKGSGDIIEEIDKLSAELRRNGC
                     VNSIFFETLLKQGFMFDMINYNKVAPSASQKSYVYVLHAEDSGLTKIGFSRRVNKRIS
                     EISRMSGGKLNLIAKIPADRELETKLHQKYYNYRSHGEWFSLNRCHLKELKEMPGNEL
                     K"
     gene            4912..5286
                     /locus_tag="RH033_002243"
     CDS             4912..5286
                     /locus_tag="RH033_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2991420.1"
                     /translation="MNDQQIEKEIVEKGKTAPRVTPEKIEGLICSEHFFTAAQGDHQA
                     KEDDLIYNPEPYVEATPDALHLLTFCVLVLKNGFTVTGESACASPENFDAEIGRKIAR
                     QNAVNKIWMLEGYLLKQKLSEQ"
     gene            5390..5632
                     /locus_tag="RH033_002244"
     CDS             5390..5632
                     /locus_tag="RH033_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HDV2991421.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIGTDELKLEL
                     FKIHFQSGGANSDITIRTFEAVRKSKEALDLFTTGA"
     gene            5635..6075
                     /locus_tag="RH033_002245"
     CDS             5635..6075
                     /locus_tag="RH033_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDV2991422.1"
                     /translation="MARPTKYQEAYAEQARKLCLLGYTDAELADFFEVSESTINKWKL
                     DYPKFSESIKKGKAVADAEVSDRLYQRAMGFVAPDIDIRVIENRIVETPLEKYYPPDT
                     TAAIFWLKNRQKDKWRDKVDHELTGKDGGAIQIETSPMSTLFGK"
     gene            6072..7487
                     /locus_tag="RH033_002246"
     CDS             6072..7487
                     /locus_tag="RH033_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HDV2991423.1"
                     /translation="MTSINPIFEPFIEAHRYKVAKGGRGSGKSWAIARLLVEAARRQP
                     VRILCARELQNSISDSVIRLLEDTIEREGYSAEFEIQRSMIRHLGTNAEFMFYGIKNN
                     PTKIKSLEGIDICWVEEAEAVTKESWDILIPTIRKPFSEIWVSFNPKNIIDDTYQRFV
                     VNPPDDICLLTVNYTDNPHFPEVLRLEMEECKRRNPTLYRHIWLGEPVSASDMAIIKR
                     EWLEAATDAHTKLGWKAKGAVVSAHDPSDTGPDAKGYASRHGSVVKRIAEGLLMDINE
                     GADWATSLAIEDGADHYLWDGDGVGAGLRRQTTEAFSGKKITATMFKGSESPFDEDAP
                     YQAGAWADEVVQGDNVRTIGDVFRNKRAQFYYALADRLYLTYRAVVYGEYADPDDMLS
                     FDKGAIGEKMLEKLFAELTQIQRKFNNNGKLELMTKVEMKQKLGIPSPNLADALMMCM
                     HCPESAAQPDYSSYSIPCGVG"
     gene            7489..9687
                     /locus_tag="RH033_002247"
     CDS             7489..9687
                     /locus_tag="RH033_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001734492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HDV2991424.1"
                     /translation="MAEKKMTDWHRKVLCNFDNAWSATQDMREQIIEAQRFVRVSGAQ
                     WEGSTNAGYSFDEGRFEHYPRFELNKIARECDRIIGEYRQNRISVKFRPKDDKASEAL
                     AEKMNGKFRADYQETSGGEACDNAFDDAVTGGFGCFRMCADYEDEMDPSNEQRRISLL
                     PVYDPATCVFFDQDSKQYDRSDAMWAMEMFSMTPKAFEAEYPDSIAASLSRDDTGTQY
                     DWSTPDAIYVGRYYEVRIEKVKLTAWRNPVSGETAIYDEEQIKDIVDELTDGAFELIG
                     ERTVKKRRVYCGLLSGAEWLEEPKRIPGEHIPLIPVYGRRSFVDNQERIEGHAAKAMD
                     AQRLENLMVSMIADNATQAGGDGIPVVDVDMIPGPLATHWAERNKKRPAFLPMVSLKN
                     KNGDITAQAQVSSYTPPTQMPPALAGLLQYTGTAIQQITGASQLENMPSNVATDTVDS
                     IFNRMDTQSYIYMDNMAKSMRRAGVVWLSMAREVYGSDTPMRIVNEDGSDDVALMTGE
                     VVDRQTGRVIALNDLSQGNYEVTVDVGQSFATRRDATVKSLLSMLALIPPGTPKHDLV
                     SSMILDNMDGEGMDDLKEYNRNQLLLSGVIKPRTPEEQQMVEQAKQQQASQPDPAMVA
                     AQGQLLAGQAELQKAQNEQAAIQVKAFQAQTDAQVAAANVVKILASADSQQKSDIREA
                     LKLLGQFQQQQGDNARADAELVLKSQAQGHAQRMDISSILQKSTQQQPQQ"
     gene            9778..10671
                     /locus_tag="RH033_002248"
     CDS             9778..10671
                     /locus_tag="RH033_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001734493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HDV2991425.1"
                     /translation="MDQMAENTPEVEIETDASEQIPDDVELAEEVETEDGSESSGNDA
                     EEATETDDDESEQEFYFGDEKLDSPTSEDGAEHGLVKHLRKTIKEKDRELKELMRQSQ
                     KPVEQQPVITQPPRMPKLDDEDIGFDEEIYQQRMAKWAEDNGKYQQQEMARKQKEQEL
                     QAAYQERLSKYQQRVKALKVPGYQEAEQAVLEEIPIETQNAILFESEKPEIVVLALGR
                     NAELRKQLAEATNPVAIGRLLERIESKARIMPKAKTTAATTPTVKGSNGAVINNLDKL
                     KAKALETGDWTPYFAAKKAKK"
     gene            10690..11943
                     /locus_tag="RH033_002249"
     CDS             10690..11943
                     /locus_tag="RH033_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HDV2991426.1"
                     /translation="MANQLAKDLEIMFENYVEGFEAACVVSRNAKKFRPGDTAMQRAG
                     DVLYRPQHYHMNIEEGLDLSGKTPTALVQRLVPSVFKEPKNILYTLDAREMRDPEHKT
                     EAGRAAGMRLAAQIDSDLISMVAQRATNVITMADSTTGTQGRDLWNCAAGIDATMTAI
                     GVPQGINRRSFWNPFNYKDLAGELGHRAYAQGATLTAYEKAQIPPVASFDSYKTDISG
                     RLPKGSTESLTVSGQPEHKVEAKDSNGMPVDNRQGTITVSASGLQVGDAFTIAGVNSV
                     HQITKDTTGQPQVFRVLAVSGTTVTISPKILPVENTDVASRPYANVDAKPAESAAITI
                     LNKNAAPANLFWADGSVELMYGKLAFPTGQGPQVMTATTEQGATLIMSYAFDHIKGVT
                     TARFTTLYGCSVLVPEYTGIVIAGQ"
     gene            11985..12173
                     /locus_tag="RH033_002250"
     CDS             11985..12173
                     /locus_tag="RH033_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2991427.1"
                     /translation="MARTMLYKPGNMITCGQFAVDYIIVDDEEVKSHLKKGWVKTPEE
                     TATKQKVAKAEEDGENEG"
     gene            12154..12615
                     /locus_tag="RH033_002251"
     CDS             12154..12615
                     /locus_tag="RH033_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head DNA stabilization protein"
                     /protein_id="HDV2991428.1"
                     /translation="MAKTKGDLVLKALRKAGLYSNATLTDADPQEIEDAINDLEDMMA
                     AWQAKGIELGYQFADTENGIMPLPDDDSGIPAWANDGVALKLAVQVCMDNVIQPSDAL
                     LTAADSAYQTICIALTKIPPLERRNDMPRGSGNKSAFTWNRFYIEKDDPST"
     gene            12625..14043
                     /locus_tag="RH033_002252"
     CDS             12625..14043
                     /locus_tag="RH033_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HDV2991429.1"
                     /translation="MPIKQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIGSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFIYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DFEVESSTGVAQYADRLFLSATTDGINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKFRVITKSPVTLSGCQIRIE"
     gene            14043..14891
                     /locus_tag="RH033_002253"
     CDS             14043..14891
                     /locus_tag="RH033_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2991430.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERISQAESTLQEHE
                     TRISQNESDIASLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGTLAPFFDTVNDKMVVFNENKTLFFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            14891..15346
                     /locus_tag="RH033_002254"
     CDS             14891..15346
                     /locus_tag="RH033_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HDV2991431.1"
                     /translation="MIQFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTFDCHAMYLPEVRGFSKEIGMAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            15349..16041
                     /locus_tag="RH033_002255"
     CDS             15349..16041
                     /locus_tag="RH033_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HDV2991432.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSQQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            16051..17457
                     /locus_tag="RH033_002256"
     CDS             16051..17457
                     /locus_tag="RH033_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HDV2991433.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEDQRNSIGTLAAGARLASSSPEAMQSWLQNNAKELTRVGVDPNSVA
                     QMYQQNPSGFGEFVDHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGEALQ
                     ARGQNLSYQSAMTGHNIAAQRLALDQQEFGFKMQQAQEKAQQLISEAPKLSVNMEKGI
                     ETAVNNATASSNSANSMSALAQQFRAEKPTTGLFGNAQNMFAKLTGSDTTLRDLRIRQ
                     NALVNSQVLKFLPPGPATDKDVEIVRQGAPTDMDNPETVARWLDAMANLERRNAQFNE
                     FKAEWMSANGNPGQSRNGGQILGLDVKKGESLGSAVKRYMSMNTDAAPAQDSTPSGET
                     PNQVGSYTSKSGIQFTVE"
     gene            17457..19295
                     /locus_tag="RH033_002257"
     CDS             17457..19295
                     /locus_tag="RH033_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HDV2991434.1"
                     /translation="MKVTANGKTFTFPDGTSTEDIGSAIDEYFAGQSVQQQTVSQDSN
                     EPAREEPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGG
                     PKVLDDVYRPVDRPTDPYAQTGETIGGYLVPGVGTAGSMAIGSLAEAANQKGDFAQNA
                     AKNAGVNLAAQGVLSAAAKGIGRGITAIKGDIAPEVAKKIATSESMGVTPMTSDVIPP
                     KNAFTRGLTQDAEGALLGTGSKRAEQYATRSKLVSNYFDRFGEYNPDDVVKSLTTTLR
                     GRKDAAGAVINDVTNKMGNAAVDTTNTMNALNTAIARQERLGTSANQSLLTSLRNLRE
                     ELANPATDLDVTFDLLRQHRTAFRSNVQGDAMVFPNQAKAATNMVENAMSKDLRNAVA
                     KNLGASDAAKYLKANSDYANVYNKVLNKNIANKLNKASSEASPELINTVVLSRKPSDV
                     KRIWSALDDKGKDAMRAAYVSKIAEKAGDSPAKFITEVNKLKSQSGGEIYNTIFSGKH
                     MKELDALHEVLQQTARSDTANVVTQTGQSQANRIRTIGATATLGVSMGLEAGFGAMMR
                     LYESKAARNMLLRLANVKPGTPAYERALNQAANAVRPLLTNEATRQ"
     gene            complement(19315..19644)
                     /locus_tag="RH033_002258"
     CDS             complement(19315..19644)
                     /locus_tag="RH033_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2991435.1"
                     /translation="MLEIVVLALGISCCVLYAGLAALKKQVKELDRSHEIDTKIARLT
                     EENKHLKNSIRALTDDNYKLSHALAKWEIVSYERMTDMIFSSYMATKSPETSGKAIIA
                     AIEKRIK"
     gene            20019..20462
                     /locus_tag="RH033_002259"
     CDS             20019..20462
                     /locus_tag="RH033_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SocA family protein"
                     /protein_id="HDV2991436.1"
                     /translation="MLTCFDVADYFLSRCDEDSGDTISNLKLQKLVYYAQGFSLALLG
                     EPLFQNKMEAWMHGPVVPELYRRYKQYGNGAIPSPESFDAEKFSEEQLELLEEVWDVF
                     GQFSAWKLRNMTHEESPWRSNYIEGVGGSEISSKEMAEYFATRIN"
     gene            20443..20886
                     /locus_tag="RH033_002260"
     CDS             20443..20886
                     /locus_tag="RH033_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2991437.1"
                     /translation="MQLGLTNEMARKSKRIIPPAESTGKLKLGPQSGGNSDQKKPKFS
                     FCYIQSSHCITKCQKDEKAGLADKLYRLSQLTWAEIKQQGRHKLGFEKIARGAIKAGI
                     PSHITEDVDHFLAFRFDDLKAMVGYRLGSTFFVIWLDREFNLYKH"
     gene            complement(20899..21150)
                     /locus_tag="RH033_002261"
     CDS             complement(20899..21150)
                     /locus_tag="RH033_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HDV2991438.1"
                     /translation="MARDDPHFNFRMPMEVREKLKFRAEANGRSMNSELLQIVQDALS
                     KPSPVTGYRDDAERLADEQSDLVKKMVFDTLKDLYKKPT"
     gene            21241..21402
                     /locus_tag="RH033_002262"
     CDS             21241..21402
                     /locus_tag="RH033_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HDV2991439.1"
                     /translation="MKGMSKMPQFNLRWPKEALDLVRKVAEENGRSVNSEIYQRVMES
                     FKKEGRIGA"
     gene            21471..22373
                     /locus_tag="RH033_002263"
     CDS             21471..22373
                     /locus_tag="RH033_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HDV2991440.1"
                     /translation="MNSIAILEAVNTPYVPFNGQQIITAMAAGVAYVAMKPIVENLGM
                     SWSTQQTKLMKQISKFNCVHMNMIAADGKLRKLLCLPLKKLNGWLFSINPEKVRADIR
                     DKLIQYQEECFTVLHDYWTKGKAENARKKTSVDDRTPLRDAVNMLVSKKHLMYPEAYA
                     MIHQRFNVESIEELDASQIPQAVEYIHRVVLEGEFIGKQEKKANELSAKEANSLVWLW
                     DYANRSQALFRELYPALKQIQSNYSGRCYDYGHEFSYVIGMARDVLINHTRDVDINEP
                     DGPTNLSAWMRLKNKELPPSVHNY"
     gene            22473..23882
                     /locus_tag="RH033_002264"
     CDS             22473..23882
                     /locus_tag="RH033_002264"
                     /inference="COORDINATES: protein motif:HMM:NF015000.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2991441.1"
                     /translation="MTDSINANVVVSMPSQLFTMARSFKAVANGKIYIGKIDTDPVNP
                     ENQIQVYVENEDGSHAPVSQPIIINAAGYPVYNGQIAKFVTVQGHSMAVYDAYGAQQF
                     YFPNVLKYDPEQFKDTLHSASGADNVGYRGYTVSEYLSHTALSFGNLNDCLSHDKYKI
                     GDKVFVHGYNYPSDGGMASFEVVSSDEPVGIKTYDGKFKLKRTSGHNYLELIIKNGAT
                     PKIIAHRGWAGITPNEYQGALTAPVYFVPENTRASIRFCAERGAFGVEGDTKITLDGV
                     PVIFHDETVDRTTNGTGAVSTFTFLELKALDAGSYVSGIYADEKILNYDEWIRECKRR
                     GVMPFVEWSAPMSVTQADNFLETINKYYGTKPTNVFVYSTYPAVLELLRSKNAYIGLG
                     VIPSYGVAPTTEQLELAYKLGNCGISLSGGIITNAAVVNSVKKYGLLLIYAIANTPAR
                     IDNCISAGVDIMITDCYRG"
     gene            23884..24669
                     /locus_tag="RH033_002265"
     CDS             23884..24669
                     /locus_tag="RH033_002265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2991442.1"
                     /translation="MNIKTYRQIHGGMRSFVTTSLFPVGANKFDKSDARKSVLSKTSS
                     DSAALEVMDLGWLAAGTCISFCCDIKINTQMSGAPSISIESRNGARLSDGRKIVNIGT
                     TNSKEWETVRCDWIVSEFDNWITLSYGASTPVLGQFEFRNPRISITNDQARLPINFSL
                     LRSGNVWRVDTGTFIYNGAVLVSANGDSIEVSWPYVNNIRSPIVHTTIDTGFSLLSAG
                     CISCGPHSVSNSGCKIRLIKPDGSFVTDVTNGTTLRIHVSVNY"
     gene            complement(24795..25949)
                     /gene="intS"
                     /locus_tag="RH033_002266"
     CDS             complement(24795..25949)
                     /gene="intS"
                     /locus_tag="RH033_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HDV2991443.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYANELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQIPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDANVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NENEWPADAIEVQLAHASGGSVRGIYNHAQYLDKRREMMQWWADWIDEKV"
     gene            complement(26111..26185)
                     /locus_tag="RH033_002267"
     tRNA            complement(26111..26185)
                     /locus_tag="RH033_002267"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26150..26152),aa:Arg,seq:cct)
     gene            complement(26261..27193)
                     /locus_tag="RH033_002268"
     CDS             complement(26261..27193)
                     /locus_tag="RH033_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDV2991444.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            27487..28242
                     /gene="mlaA"
                     /locus_tag="RH033_002269"
     CDS             27487..28242
                     /gene="mlaA"
                     /locus_tag="RH033_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HDV2991445.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(28304..29644)
                     /gene="fadL"
                     /locus_tag="RH033_002270"
     CDS             complement(28304..29644)
                     /gene="fadL"
                     /locus_tag="RH033_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HDV2991446.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIRQSPAWLTPQGQALAATANSIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNPAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            30016..30300
                     /locus_tag="RH033_002271"
     CDS             30016..30300
                     /locus_tag="RH033_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDV2991447.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            30482..31792
                     /gene="fadI"
                     /locus_tag="RH033_002272"
     CDS             30482..31792
                     /gene="fadI"
                     /locus_tag="RH033_002272"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HDV2991448.1"
                     /translation="MGQVLPLITRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAIANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLHPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     ADLTLIDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            31792..33936
                     /gene="fadJ"
                     /locus_tag="RH033_002273"
     CDS             31792..33936
                     /gene="fadJ"
                     /locus_tag="RH033_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HDV2991449.1"
                     /translation="MEMASAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIANCKTAQEAEALARQGQQLMAEIHALPV
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQAVKLGLVDDVVPHSILLEAAVELAKQDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVIENGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAQPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDRQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDTALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGTQGQGRLSAPQVAERCVM
                     LMLNEAVRCLDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMSKRGESFWKTTATDLQ"
     gene            34139..34624
                     /gene="sixA"
                     /locus_tag="RH033_002274"
     CDS             34139..34624
                     /gene="sixA"
                     /locus_tag="RH033_002274"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HDV2991450.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
BASE COUNT         9447 a         7885 c         8826 g         8564 t
ORIGIN      
        1 ccagacctct ccgcgccgcc atgattgtct ttctcatgat gcaggacgcc aataatgctt
       61 agcccatccc aatcccttca ataccagaaa gaaagcgtcg agcgagcttt aacgtgcgct
      121 aactgcggtc agaagctgca tgtgctggaa gttcacgtgt gctccgattg ctgcgcagaa
      181 ctgatgagcg atccgaatag ctcaatgtac gaggaagaag acgatgagtg atgttaaaga
      241 aaaagatatc cccggctttg agggtatata taaagtaact gaaaatggag acatcatttc
      301 atgccgtaaa tcaaaaaaat tatctcatgg cattaaacca ggaggatatg catttgtcgg
      361 tctgtatcca ggtggcggga aaagaccatc atataaaatg gttcacagaa ttgttgcaga
      421 agtatttatt gataacccag atggcaaacc ggaagttaat cacaaggatg gaaataaact
      481 taataataaa gttgaaaatc ttgattgggt aacgcgaaca gaaaatgcga aacatggatt
      541 tgattccgga ttgcttgttc atgggtttaa tcatcacttc tgcaaactaa cgccagaaca
      601 agtgaaatca atatataaat caaaaggcaa atacagagat atagccaaag aatttggtgt
      661 ttgtgcgcag acagtgtgca acataaaaaa caaatcagcg taccgacgtt ttttggaggg
      721 gattgatgtt tagaagcaaa aaatggcttc aggcagtcag ggatattgaa ttttgcgttc
      781 tttgcggaag atacggagtt caggccgctc acagaaatga agggaagggg gttgggatta
      841 aagtagatga ttgccttact gctgcgctat gtgttgattg tcattcaaga attgataatg
      901 ggagagatat gagcagggaa gagcgaaggg ctgaaatgga tcgggccatt gtgcttaccc
      961 ttaaaaaatt ggttaacaat gggagggtgt ttgtccaatg aacgaatatc agtttgtgct
     1021 tccatacccg ccgtcggtga atacctactg gcgaagacgg ggaagccaat attacataag
     1081 cgataaaggc cagaaatacc gaaaagacgt tcagcaaatc atccgccaac tcaagttaga
     1141 cattttcacc aaatcacgac tccgtatcaa agtcatcgca gacgttccag actcccgccg
     1201 ccgcgacctc gacaacatcc tgaaaggttt actcgactcc cttatccacg ccggatttgc
     1261 ggaagacgac gagcaattcg atgacattcg cgtaattcgt ggtgtgaaag taccaggcgg
     1321 acggcttgga ataaaaatca ccgaactgga gaacgcatga acgccacaat tcaaacgata
     1381 ccagagcttc ttatccagac acgaggcaat cagaccgaag tggagaggat gctttcctgc
     1441 gcaagaggaa cagtgctcaa gtacaaccga gacagcaaag gcgagcgtca cgtaatagtt
     1501 aacggcgtcc tgatggtcaa acagggcaag aggggaagac catgagactc gaaagtgtag
     1561 ctaaatttca ttcgccaaaa agcccgatga tgagcgactc accacgagcc acggcttctg
     1621 actctctttc cggtactgat gtgatggctg ctatggggat ggcgcaatca caagccggat
     1681 tcggaatggc tgcattctgc ggtaagcatg aactcagcca gaacgacaaa caaaaggcta
     1741 tcaactatct gatgcaattt gcacacaagg tatcggggaa ataccgtggt gtggcaaagc
     1801 tcgaaggaaa tactaaggca aaggtactgc aagtgctcgc aacattcgct tatgcggatt
     1861 attgccgtag tgccgcgacg ccgggggcaa gatgcagaga ttgccacggt acaggccgtg
     1921 cggttgatat agccaaaacg gagcagtggg ggagagttgt tgagaaagaa tgcggaagat
     1981 gcaaaggtgt cggctattca agaatgccag caagcgccgc atatcgcgct gtaacgatgc
     2041 taatcccaaa ccttactcaa cccacctggt cacgcactgt taagccgctg tatgacgctc
     2101 tggtggtgca atgccataaa gaagagtcaa tcgcagacaa cattttgaat gcggtcacac
     2161 gttagcagca tgattgccac ggatggcaac atattaacgg catgatattg actttttgaa
     2221 taaagttggg taaatttgac ccaacgatgg gttaattcgc tcgttgtggt agtgagataa
     2281 aaagaggcgg cgcttactac cgattccgcc tagttggtca cttcgacgta tcgtctggaa
     2341 ctccaaccat cgcaggctga gaggtctgca aatgcaatcc cgaaacagtt cgcaggtaat
     2401 agttagagcc tgcataacgg tttcgggatt ttttatttgg gtcagtcgta taaaggtcat
     2461 tacggaaggc tgttaacctt cttatcgtgg ttcgagtcca cgctgtcccg ccaaacatgc
     2521 tggtttagct ctaatggtag agcagtcgcc ttgtaagcga atgggtagcg gttcaagtcc
     2581 gttaaccagc accataactg agccgtagcc actggctatt ctgaattcat cagtgatagt
     2641 tacgctgcgg ccttctacac atgatcttcg tgaaagcggg tggcatgagg ttgcgctaac
     2701 aacctcctgc cgttttgccc gtgcatatcg gtcacgaaca aatctgatta ctaaacacag
     2761 tagcctggat ttgttctatc agtaaccgac cttattccta attaaataga gcaaatcccc
     2821 tcaatgaagg ggtagagcat gtaccgtatg gacaaaatca gagaatggtt cagttacagc
     2881 ttcggaggac tgactgcgat gggtggcatt ctctccctga atgactgggc tgtcatcatt
     2941 ggtattcttt gtactgtcgg cacatttggc atcaactggt actacaagcg caaagagcgc
     3001 gaggacagat tgaatggcaa tgtcaccggc actacgaaat agcgtaatag cggcgataag
     3061 tggcggggct attgctatag catctgtgtt aatcactgga ccaagtggta acgatggtct
     3121 ggaaggtgtc agctacatac catacaaaga tattgttggt gtatggactg tatgtcacgg
     3181 acacaccgga aaagacatca tgctcggtaa aacgtatacc gaagcagaat gcaaagccct
     3241 cctgaataaa gaccttgcca cggtcgccag acaaattaac ccgtacatca aagtcgatat
     3301 accggaaaca acgcgcggcg ctctttattc gttcgtctat aacgtgggcg caggcaattt
     3361 cagaacatcg acgcttcttc gcaaaatcaa ccagggcgat atcaatggcg catgtgacca
     3421 gctacgtcgc tggacatacg ctggcggtaa gcaatggaaa gggctgatga ctcgtcgtga
     3481 gattgagcgt gaagtctgtt tgtgggggca gcaatgagca tgatttgctt tttcatggca
     3541 gcgttgctcg catttaatgg caacgatgcg tggccgtggt ttctggccgt tggggtgttg
     3601 atgtcatgag tcggttaacc gcgattatct ccgctctggt tatctgcatc gtcgtctgtc
     3661 tgtcgtgggc tgttaatcat taccgtgata acgccatcgc ctacaaagag cagcgcgaca
     3721 aagccgcatc catcatcgct gacatgcaga agcgtcaacg tgatgtagca gaactagacg
     3781 ccagatacac aaaggagctt gctgatgcta acgcgactat cgaaagtctc cgtgctgatg
     3841 tttctgctgg gcgtaagcgc ctgcaagtcg ccgccacctg tgcaaagtca acgaccggag
     3901 ccagcggcat gggcgatgga gaaagcccag gacttacagc agatgctgaa ctcaattatt
     3961 accgtctccg aagtggaatc gacaagataa ccgcgcaggt taactacctg caggaataca
     4021 tcaggacgca atgcctgaaa taattttttt gcaaatcaca aagtccattt aatgagcctc
     4081 gcgatgcggg gcttttttta catctgaatt tcacagcgca tctcacgcgc atattacatc
     4141 acccgagcct ttcagaaagt tgagcctgag aactgccgta tatggtggcg accatctcgg
     4201 ggcggctttt ctgtgagaca ggctcatctt tctaaaaggt aaacgctatg aataactttg
     4261 ttgaaattac ctcaagaatt ggtcgcatgt accaagattt tcttataagt ggaaaggggt
     4321 ctggcgacat catagaggaa attgacaagc taagtgcaga gctgagaagg aatgggtgtg
     4381 ttaattctat cttttttgaa actttgctaa agcaaggctt catgtttgac atgattaatt
     4441 acaacaaagt cgcacccagt gcttcgcaaa aatcatatgt gtacgttctg catgctgaag
     4501 atagtgggct aacaaaaata gggtttagtc gcagggttaa taaacgaatt tcagagatat
     4561 ctcgcatgag tggtgggaag ctaaatctaa ttgcaaagat tccggccgat agagagcttg
     4621 aaaccaaatt gcaccaaaaa tattacaact ataggtcgca cggggagtgg tttagcctca
     4681 atcgttgtca tttgaaagag ctaaaagaaa tgcctggtaa cgaactgaaa taatccccgg
     4741 actcaccaat taacggcagt acagcgaaac aacccaagcc agtaagtggg gaaataacac
     4801 tggcagccac tgaaagatga acctcctgcc tgatggcaaa aaagattctt tgtggtggcg
     4861 ggctgatgga aagacatcct aatcaagcaa ccactccaca gggtaataat tatgaacgac
     4921 cagcaaatcg aaaaagaaat cgttgagaaa ggcaaaacgg caccgcgtgt aacacccgag
     4981 aaaattgaag gcctcatttg cagtgagcat tttttcactg ctgcgcaggg tgaccaccag
     5041 gctaaagaag atgaccttat ttataatccg gagccttatg tcgaagccac ccctgatgct
     5101 ctgcaccttc tcactttctg cgtattggta ctgaagaatg gcttcaccgt taccggagag
     5161 agtgcctgtg caagcccgga aaactttgat gcagaaattg gtaggaagat tgcccggcaa
     5221 aatgctgtaa acaaaatctg gatgctcgaa ggttacttgc tgaagcagaa gctaagcgaa
     5281 cagtagttat tacaaaagcc attccctaca gagtggcttt gataatggct tataccctac
     5341 acgggataac ttaactgata tcccttttaa cggataaacg gagccaacaa tggcagagat
     5401 tattcccatg actgaagaac agaaattcca gttagagatt tacaaactgg tcatgaacca
     5461 gaacgcagcc gcagaagaag catttcagtt catcggcact gacgaactga agcttgagct
     5521 attcaaaatt cacttccagt caggcggcgc taattcggat atcacgatcc gcacattcga
     5581 agcggtgcgt aaatcgaagg aagcgttaga cctgttcacc accggagcat aaacatggcg
     5641 cgcccaacaa agtatcaaga ggcgtatgcc gaacaggcac gcaaactgtg cttgctgggc
     5701 tacaccgatg cagagcttgc tgatttcttc gaagtcagtg agtcaactat taacaagtgg
     5761 aagcttgatt atcctaagtt ttcggagtcc ataaaaaagg gtaaggccgt cgctgatgca
     5821 gaagttagtg atcgtcttta tcaacgcgct atgggcttcg tggctccaga catcgatatt
     5881 cgtgttattg aaaacagaat tgtcgaaact ccgcttgaga agtattaccc gcctgataca
     5941 accgctgcca tcttctggct taagaatcga cagaaggata aatggcgcga caaggttgat
     6001 cacgaactaa caggcaaaga cggcggcgca atccagattg aaacatcacc gatgagcact
     6061 ctattcggaa aatgacctcg attaatccta tctttgaacc gttcattgag gcgcatcgct
     6121 acaaagtcgc caaaggcggt cgaggtagcg gtaaatcatg ggcaattgcg aggctgcttg
     6181 ttgaggcggc acgtcggcag cctgtgcgta ttctctgcgc tcgtgaactg caaaacagta
     6241 tcagcgattc ggtaatccgg ttgcttgaag ataccatcga gcgtgaaggg tattcggctg
     6301 agtttgaaat tcagcgttcc atgattcgtc atctcggaac gaatgctgag ttcatgttct
     6361 acggcatcaa aaacaacccg acgaagatta aatcgctaga aggtattgat atctgctggg
     6421 tggaggaagc ggaagcggta acgaaggaat catgggatat cctgatacca accatccgca
     6481 agccgttttc cgaaatatgg gtgagcttta accctaagaa catcatcgac gatacctatc
     6541 agcggttcgt cgtaaaccct cctgatgata tttgtctgct gacggtgaac tacaccgaca
     6601 acccgcactt tcctgaagtt ctacgtctgg agatggaaga gtgtaaacgc agaaatccga
     6661 cactgtatcg tcacatctgg cttggtgagc cagtaagcgc aagtgatatg gcaatcatca
     6721 aacgtgaatg gcttgaagcc gcaaccgatg cgcacacgaa actcgggtgg aaagcgaaag
     6781 gtgcggttgt ttctgcgcat gacccatcag atacagggcc agatgctaaa ggttatgcat
     6841 cgcgtcacgg ttcggtagtt aagcgcattg ccgaaggtct gctgatggac attaacgagg
     6901 gtgctgactg ggctacttcg ctggcgattg aagacggcgc tgaccattac ctgtgggatg
     6961 gtgatggtgt tggtgccggg ctacgcagac agacaacgga agcgttctcc ggcaagaaaa
     7021 tcaccgccac gatgttcaag ggcagcgaat cgccattcga tgaagatgcg ccgtatcagg
     7081 ccggagcatg ggctgatgaa gtcgtacagg gtgacaacgt tcgcactatt ggcgatgtgt
     7141 tccgcaataa gcgagcgcaa ttctattacg cgctggctga caggctgtat ctgacatatc
     7201 gggcggttgt ctacggtgag tatgcagacc cagacgacat gctgagtttc gacaaaggag
     7261 cgataggcga gaagatgctg gagaagctgt ttgcagagct gacgcagatt cagcgcaaat
     7321 tcaataacaa cgggaagctg gagctaatga ctaaggtcga aatgaagcag aagctcggta
     7381 ttccatctcc taacctggct gatgcgctga tgatgtgtat gcattgcccg gagtcggctg
     7441 cgcaacccga ctattccagt tactcaattc cttgtggtgt aggttgatat ggcagaaaaa
     7501 aagatgactg actggcatcg caaggtgctg tgcaactttg ataatgcctg gtcagcaacg
     7561 caggatatgc gtgagcagat tattgaggct caacgtttcg tccgggtatc cggcgcacag
     7621 tgggaaggca gcacaaacgc tggttactca tttgatgaag gcaggtttga gcattacccg
     7681 cgctttgaac tgaataagat tgcccgtgaa tgtgatcgca tcattggcga gtatcgacag
     7741 aatcgcataa gcgttaaatt caggccgaag gacgataagg catcggaagc gttagccgaa
     7801 aagatgaacg gcaaattccg cgctgactat caggaaacat ccggtggcga agcgtgtgat
     7861 aacgcatttg atgatgctgt aacgggcgga ttcggttgtt tccgcatgtg tgccgattac
     7921 gaagatgaaa tggatccgag taacgagcag cgacgcatca gccttcttcc tgtttacgac
     7981 ccagcgacat gcgtcttctt cgatcaggac agcaagcaat atgaccgctc tgatgctatg
     8041 tgggctatgg aaatgttctc catgacgcct aaagcgttcg aagctgaata ccctgattcc
     8101 atcgctgcaa gtctttctcg tgatgacact ggcactcaat atgactggtc aactcctgat
     8161 gctatctatg ttggtcgcta ctatgaagtc cgcatagaga aggtgaagct cacggcgtgg
     8221 cgcaaccctg ttagcggaga aacggcaatc tatgatgaag agcaaatcaa agatattgtc
     8281 gacgagctaa ccgatggtgc attcgaactg atcggtgagc ggacggtgaa gaaacgcaga
     8341 gtttattgcg gtcttctgtc tggcgctgaa tggctggaag aacctaagcg tattccgggc
     8401 gaacatattc ctctcatccc ggtatatggg cgtcgctcat ttgttgataa tcaggagcga
     8461 atcgaaggcc acgcagcaaa agcgatggat gcacagcgtc ttgagaacct gatggtttcc
     8521 atgattgcag ataacgctac tcaggctggc ggtgatggca ttcctgtagt tgatgttgac
     8581 atgattcctg gtcctcttgc cactcattgg gcggagcgca acaaaaagcg cccggcgttc
     8641 ctgccgatgg tcagtctgaa aaacaaaaac ggagatatta ctgcgcaggc tcaggtcagc
     8701 agttatacac ctccgacaca aatgcctcct gctcttgccg ggctattgca gtacaccgga
     8761 acggctattc agcaaattac aggtgcgtcg cagcttgaga acatgccgag caacgtcgct
     8821 accgataccg ttgatagcat ctttaaccgg atggatacgc agtcctatat ctacatggac
     8881 aacatggcta aatccatgcg tcgcgctggc gttgtgtggc tttctatggc gcgtgaagtc
     8941 tatggcagcg atacgccgat gcgtatcgtt aatgaggacg gcagcgatga cgtggcgctg
     9001 atgactggtg aagtggttga ccgtcagaca gggcgggtta tcgcgcttaa cgacctttcg
     9061 cagggtaact acgaagtgac tgtcgatgtc ggtcagtcgt tcgctactcg ccgtgatgca
     9121 acggttaagt cgttactttc catgctggca cttatcccgc cagggacgcc gaagcacgac
     9181 cttgtatcgt cgatgattct cgacaatatg gacggcgaag ggatggacga ccttaaagaa
     9241 tacaaccgca atcagttgct tctgtctggc gttatcaagc cgagaacgcc tgaagaacag
     9301 caaatggttg agcaggcgaa acaacaacag gccagtcagc cagatccggc tatggttgca
     9361 gcgcaaggtc agcttcttgc tggtcaggct gaattgcaga aagcgcagaa cgaacaggca
     9421 gccattcagg ttaaagcatt ccaggcacag actgatgctc aggttgcagc ggcaaacgtt
     9481 gtgaaaatcc tcgcatctgc tgatagccag cagaaatctg atatccgcga ggctctgaaa
     9541 ctgctcggac agttccagca acagcaagga gacaatgccc gtgctgatgc agagcttgtc
     9601 ctgaaaagtc aggcacaggg ccatgcgcag cgcatggaca tcagcagcat cctgcaaaaa
     9661 tcaactcagc aacaaccaca gcagtaatta acccataacg tgcaatggct gtctttatga
     9721 ggcctggcac cctattgcct tccgatgggc tgaacatcga gtaaacaggg gtaacaaatg
     9781 gaccagatgg cagaaaacac accagaagtt gaaatcgaaa ccgacgcgtc agagcagatt
     9841 cctgatgatg tcgaactggc tgaagaagtc gaaacagaag atggcagtga gtcctccggc
     9901 aatgatgcag aggaagctac tgaaactgat gacgacgaat cagagcagga attctacttt
     9961 ggtgacgaaa agctggattc gccaaccagc gaagatggcg cagagcatgg actggtaaaa
    10021 cacctgcgca agacgattaa agagaaagac cgcgaactga aagagctgat gcgtcagtct
    10081 cagaaacccg tcgagcagca gccggtaatc actcaaccac cgcgaatgcc aaaactggat
    10141 gatgaggaca tcggtttcga tgaagaaatc taccagcaac gcatggctaa gtgggcggag
    10201 gacaacggca aataccagca acaggagatg gctcgcaagc agaaggagca ggagcttcag
    10261 gctgcctatc aagagcgatt atccaaatat cagcaacgtg ttaaggctct caaggttcct
    10321 ggctatcagg aagctgaaca ggccgtactc gaggaaatcc ccatcgagac acaaaacgcg
    10381 atcctgtttg agtcagagaa gccggaaatc gttgttctgg cactcggtcg caacgctgaa
    10441 ctgcgcaagc aactggcaga agctaccaac cccgtagcaa ttggtcgtct gctggaacgt
    10501 atcgaatcga aggccagaat catgccaaaa gcaaaaacca cggcagccac aaccccgaca
    10561 gttaagggga gcaacggcgc agtaatcaac aacctcgaca aattgaaagc caaggcgctg
    10621 gaaactggtg actggacgcc gtatttcgcc gctaaaaagg caaaaaaata acctatcgga
    10681 gcattaatca tggctaacca attagcaaaa gaccttgaaa tcatgttcga aaactacgtt
    10741 gaaggctttg aggccgcctg cgtagtttcc cgtaacgcta aaaaattccg tcccggtgat
    10801 acagcaatgc agcgagcagg tgatgttctg tatcgtccgc agcattacca catgaacatt
    10861 gaggaaggcc tcgacctcag cggaaaaacg cctacagcac tggttcagcg ccttgttcct
    10921 tctgtgttca aggagccgaa aaacattctg tacactctgg atgcgcgtga aatgcgtgac
    10981 ccggaacata aaactgaagc tggtcgcgcc gcaggtatgc gccttgctgc acagattgac
    11041 tctgacctga tttccatggt cgcgcagcgt gctactaacg tgatcacgat ggctgactca
    11101 accacaggta cacagggccg tgatctgtgg aactgtgcgg caggtattga tgccaccatg
    11161 acggcgatag gtgtaccgca gggtatcaac cgtcgctctt tctggaaccc cttcaactac
    11221 aaagaccttg ctggcgagct tggtcaccgt gcctatgctc agggcgcaac cctgacagca
    11281 tacgaaaaag cgcagatccc tccggttgct tcctttgata gctacaagac cgatatttct
    11341 ggtcgattac cgaaaggaag cactgaatcc ttgacagtat caggccaacc tgaacacaag
    11401 gttgaagcga aagattcaaa tggtatgcca gttgataacc gacaggggac tattacggta
    11461 tctgcatctg gcttgcaggt tggtgatgcg ttcaccattg ccggtgtgaa ttccgtacac
    11521 cagatcacaa aagataccac cggtcaaccg caggtattcc gtgttctggc tgttagcgga
    11581 actaccgtaa caatttctcc aaagattctc cctgttgaaa atactgatgt tgcgagtcgt
    11641 ccatatgcaa acgtcgatgc caaaccggca gaatcagcag caatcaccat tctcaacaag
    11701 aacgcagcac cggctaacct gttctgggct gatggttctg ttgaactgat gtacggcaaa
    11761 ctggcgttcc cgactggtca gggtccacag gtaatgacag caaccaccga gcagggcgct
    11821 acgctgatca tgtcttacgc cttcgaccac atcaaaggcg taaccactgc gcgtttcacc
    11881 actctgtacg gttgctctgt acttgttcct gaatatacgg gcatcgttat tgccgggcag
    11941 taattttggt ggggcttcgg ccccattttt attgggagaa gacaatggca cgaacaatgc
    12001 tctataagcc gggcaacatg atcacctgtg gtcagtttgc tgtcgattac atcattgttg
    12061 atgacgaaga agttaaatct cacctgaaaa aaggctgggt aaaaactcct gaagaaaccg
    12121 caacgaagca aaaagtggct aaggcggaag aagatggcga aaacgaaggg tgatctcgtt
    12181 ctaaaggctt tacgaaaagc cgggctgtat tccaatgcca cgttgacaga tgctgaccct
    12241 caggaaattg aagatgccat taatgacctc gaagacatga tggcagcatg gcaggctaaa
    12301 ggtatcgagc ttggatatca gtttgctgat acagaaaacg gcattatgcc gttacctgac
    12361 gatgattcag gtatccctgc gtgggcaaat gatggcgtcg ctttgaagct cgctgtgcaa
    12421 gtgtgcatgg ataacgtcat tcagccgtca gacgctctcc ttaccgctgc tgacagtgca
    12481 tatcaaacaa tctgcatcgc tttaaccaaa ataccaccac ttgagcggcg aaatgacatg
    12541 cctcgcggta gtggtaacaa aagcgcgttt acgtggaatc ggttttacat cgagaaagat
    12601 gatccgagta cgtgaggtga ataaatgccg attaagcaac ttccgcttat gaaaggtgtc
    12661 ggcaaagact ttcgaaacgc cgactatatc gactatctgc cagtgaatat gttggctacc
    12721 cccaaagaaa tccttaacag cagcggatat cttcgttcat tcccgggcat tgccaaacgt
    12781 tctgatgtga acggagtatc gcgcggtgtc gagtacaaca tggcgcagaa tgctgtttat
    12841 cgcgtttgcg gtggcaagtt gtataagggc gaaagcgaag tcggtgacgt cgccggaagt
    12901 ggtcgtgtat caatggcgca tggtcggaca tcacaggcgg taggcgttaa tggtcaactg
    12961 gtcgaatacc gctatgatgg cacggttaaa accgtctcaa actggcctac tgacagcgga
    13021 ttcactcagt atgagttagg ctcagtccgc gacattacgc gcttacgtgg gcgttatgcg
    13081 tggtcaaaag acggaactga ttcatggttt atcactgacc ttgaagacga atcgcatcct
    13141 gaccgataca gcgcacaata tcgcgcagaa tcgcagccgg acggcatcat cggtatcggg
    13201 acatggcgag acttcatcgt ctgctttggt tcatcgacga ttgaatattt ctccctgact
    13261 ggtgcaacca ccgttggtgc tgctttgtat gtcgcacagc catcgctgat ggtgcagaaa
    13321 ggcattgccg ggacttactg caaaacgccg tttgctgact cgtatgcgtt catcagcaat
    13381 ccggcaacag gtgcgccgtc tgtatacatc atcggctccg gtcaggtatc accaatcgcc
    13441 agcgcgagca ttgagaaaat cctccgctcc tacactgctg atgaactggc tgatggtgtg
    13501 atggaatcgc tgcgatttga tgcgcatgag ttgctgatta tccatcttcc gcgccatgta
    13561 ctggtgtacg acgcatcttc aagcgccaat ggtccgcaat ggtgtgtact gaaaacaggc
    13621 ctgtatgacg atgtgtaccg cgctatcgac ttcatttacg aaggcaatca gataacgtgc
    13681 ggagataagc tggaatccgt gaccgggaaa ttgcagttcg acatcagcag ccagtacgac
    13741 aagcaacagg aacacctgct gtttactccg ttgttcaaag cggataacgc cagggtgttt
    13801 gactttgagg ttgaatcgtc aactggcgtt gcgcagtacg ccgaccgcct ttttctctct
    13861 gcaaccactg acggcatcaa ttacggacgt gagcagatga ttgagcagaa tgaaccgttc
    13921 gtttacgaca aacgcgtttt gtggaagcga gtagggcgca tcaggaaaaa tgtcggcttc
    13981 aaatttcgcg ttatcacgaa gtcacctgtc actctgtctg gctgccagat aaggattgag
    14041 taatggcgga ttcgaatctc aatgtgccgg taatcatcca agctacgcgg ctcgatacat
    14101 cagtccttcc acgcaatatc ttctcgcagt cgtatctgct ttacgttatc gcacagggca
    14161 ctgatgttgg taacgtggcg aacaaagcca acgaggccgg acagggcgct tatgatgcac
    14221 aagtcaggaa cgatgagcag gatgtgattc tcgctgatca tgagcaacga atttctgctg
    14281 cggaagcaac gcttgttaat catgaggagc gaatcagcca ggcagaatca actcttcagg
    14341 aacatgaaac acgaatatct cagaatgaaa gcgatattgc gtcgcttgat accagagttc
    14401 agtcgctgga atcgcaggtt tcagaccatg aaacgcgcat cgatgctctg gagtatgcca
    14461 ctactcgcaa gaagtcagag gttgtttact ctggcgtatc tgtaaccatc ccgacagcgc
    14521 cgaccaacct tgttagcctg ctgaaaacgc tcacgccgtc atccggcacg ttggcaccat
    14581 tcttcgacac cgttaacgac aagatggttg tgttcaacga gaacaaaacc ttgttcttca
    14641 agctgtcgat cgtcgggacg tggcccagcg gaaccgccaa caggtcaatg cagctaacct
    14701 tttcaggctc tgttcctgac acactggtaa gcagtcgtaa tgcggcgaca acaaccgaca
    14761 acatcctgtt agctacgttc ttcagcgtgg ataaagacgg ctttcttgcc acaaatggca
    14821 gtacgttaac catccagtca aatggtgcgg cgtttactgc cacaaccatc aagataatcg
    14881 cggagcagtg atgattcagt tcaaaccaac gcgaaacatc gacctgatag aagcagtcgg
    14941 aaatcaccct gacattattg ccggaagcaa caacggtgat ggatacgact acaagcctga
    15001 atgccgttac tttgaggtga acgtgcacgg tcagtttggc ggcattgttt actatcagga
    15061 gattcagccg ctgacattcg attgccacgc catgtacctg ccagaggttc gtggattcag
    15121 caaggaaatc gggatggcgt tctggcgata cattctgact aacaccaccg ttcagtgcgt
    15181 cacatcgttc gctgcgcgca aattccgcca cggtcagatg tactgcgcaa tgattggcct
    15241 taagcgagta ggaaccatca agaaatactt caaaggcgtg gatgacgtga cgttttacag
    15301 cgccacacgc gaagaactaa tcgacttcct gaatcacggg agatagccat gttatatgca
    15361 tttaaactgg gcagaaaact gcgcggtgag gaaccttatt gccctgaaaa aggcgggaaa
    15421 ggtggcagtt ctgataaaag cgcaaagtat gcagcagaag ctcagaagta tgccgcagac
    15481 ctgcaaaatc agcagtggca gacgatcatg aaaaaccttg ctccgttcac gcctcttgcg
    15541 gagcagtatg ttaaccagct tcagaacctt tccagtttag aaggtcaggg gcaggcactt
    15601 aatcagtatt acaactctca gcagtataaa gaccttgcag gtcaggctcg ttaccagagt
    15661 cttgctgctg cggaggcgac tggcggactt ggttcgacag ctacaagcaa tcaactggct
    15721 acgattgctc cgactctcgg tcagtcgtgg ttatcaaacc agatgagcaa ttacaataat
    15781 ctggcaaacg ttgggcttgg tgcgctgcaa ggtcaggcaa acgctgggca gacatacgcc
    15841 aacaacatga gcagcattgc acagcaaagt gcagcacttg ccgctgctaa tgccaataaa
    15901 ccatcaagtc ttcagactgc aattagcggt ggcacgtctg gtgcgattgc cggtgcaggt
    15961 cttgccagcc ttttgggaac atcaacgcct tggggcgctg gcattggtgc tggtatcgga
    16021 ttgcttggct cgttgtttta aggggtaatc atggctactt ggcaaggaac aaacggcgga
    16081 ttgttggctg gtatcggtgg cgtcaactca aacgctccga gcgtaaatga catcggcaat
    16141 acgcttcagc ttatcaggca gaacaatgat attgagcgtt caggcgctaa caatgttggg
    16201 ctgactgctt tgcaaggcct ttcaggtatt gcgggggtgt ttcagcagga aaagcaggct
    16261 cagcggcaga aagaatttca gcaggcatac gctaatgctt atgcgtctgg tgatcgcggt
    16321 gctttgcgtc agttggctac tcaatatcca gaccagattg aatccgttcg taaaggcatg
    16381 ggattcattg atgaagatca gcgtaattct atcggcacct tagcggctgg cgcacgcctt
    16441 gcgtcatcgt ctccagaagc aatgcaatca tggctgcaaa acaacgccaa ggaactgact
    16501 cgcgtcggtg ttgaccctaa cagcgttgct cagatgtatc agcagaatcc ttcaggattt
    16561 ggtgagtttg ttgatcacct tgggatggct gctcttggtc cgattgatta cttcaatgtc
    16621 caggacaaga tggctggtcg tgaaattgac cgaggcagac tggcagagac aatccgcagc
    16681 aatcaggctg gagaagcact tcaggcgaga gggcaaaacc tttcctatca gtcagcaatg
    16741 actgggcaca atatcgcagc acaacgcttg gctctggatc agcaagagtt cgggtttaag
    16801 atgcagcaag cgcaggaaaa ggctcagcag ttgattagcg aagcacctaa gctgtcagta
    16861 aacatggaaa aaggcatcga gacggctgta aacaatgcta cagcatcatc aaactcagcc
    16921 aattctatga gtgcgcttgc tcaacagttc agagcagaaa aaccaacgac aggtttgttc
    16981 ggtaacgcac agaacatgtt cgcaaaactt accggaagcg atacaacatt gcgtgatttg
    17041 cgcattcgcc aaaatgccct tgttaacagt caggttctta aattcctacc tcccggccca
    17101 gcaacggata aagacgttga gatcgttcga cagggtgctc caactgacat ggataaccct
    17161 gagacggtcg caagatggct tgatgcaatg gcaaaccttg agcgacgaaa cgcgcagttt
    17221 aatgagttta aagccgagtg gatgagcgcg aatggcaacc ctggacaatc gcgtaatggc
    17281 ggtcagatat tggggttgga tgttaaaaaa ggtgaatcat tggggagtgc cgttaagcgg
    17341 tatatgtcaa tgaatactga cgcagcacca gcgcaagatt cgacaccttc aggagaaaca
    17401 ccgaatcagg ttggatcata tacctcaaaa tcaggcattc aatttacggt ggaatgatga
    17461 aagtaactgc aaacggtaag acatttacct tccctgatgg tacgagcacg gaagatattg
    17521 gatctgccat tgatgagtat tttgctggac agtctgttca gcaacaaact gtcagccagg
    17581 atagcaatga accagcacgt gaagaaccat cattgatgca acaagctggc gattggctca
    17641 ctggtggtca aagtgcaggg caaattgcag aacaggctgg tcgtggtctg gtaaacatac
    17701 catttgacgt attgcagggt ggcgcaagtc tgattaatgc aatcagtcag gggcttggtg
    17761 gtccaaaagt attggatgat gtctatcgtc cagtcgatcg accgacagac ccttatgcgc
    17821 aaactggaga aacaattggc gggtatttag ttccaggagt tggaacggca ggaagcatgg
    17881 ctattggatc actggcagag gccgcaaatc agaaaggcga tttcgcacaa aatgcagcaa
    17941 aaaatgccgg agttaacctt gccgctcagg gggttctttc cgcagcagca aagggaatag
    18001 ggcgtggaat aacggctata aaaggtgata ttgcgccaga agtagcgaag aaaattgcca
    18061 catcagaatc gatgggcgtg acaccaatga catctgatgt tatcccgccg aaaaatgctt
    18121 tcactcgcgg ccttactcag gatgccgagg gggctttgct cgggacaggc tcaaagcgag
    18181 cggagcaata tgcaacgcgt agtaagctgg taagcaatta ttttgaccgt tttggtgagt
    18241 acaaccctga tgatgtggtg aaatctctga ccaccacgtt aagggggcgg aaggatgccg
    18301 ctggcgctgt tatcaatgac gtcaccaata aaatgggtaa tgccgcagtt gataccacaa
    18361 ataccatgaa tgctctgaat acagcgatcg caagacagga acggcttggg acgtctgcca
    18421 atcaaagcct gcttacatcc ttgcgtaacc tacgtgaaga attagcaaac cctgcaactg
    18481 atttggatgt tacgtttgat ctcttgcgtc agcacagaac agcatttaga tctaatgttc
    18541 agggagatgc tatggtcttc cccaaccagg caaaagcagc taccaatatg gtagagaatg
    18601 caatgtcaaa agaccttcgt aacgcagttg caaaaaacct cggtgcgtca gacgcagcaa
    18661 aataccttaa agcaaattcc gattatgcaa acgtttataa taaggtgctt aataaaaaca
    18721 ttgccaacaa gctcaacaag gcaagcagtg aagccagtcc tgaacttata aataccgttg
    18781 tattaagcag aaaaccatct gacgtgaaac gaatctggag cgcactggat gataagggga
    18841 aagatgctat gcgtgcagct tacgtcagca aaatagcgga aaaggccggg gactctccag
    18901 ccaagttcat cactgaagtt aataagctga aatctcagtc aggcggtgaa atttacaaca
    18961 ctattttttc tggaaagcac atgaaagagc ttgatgctct tcatgaagtt ctacagcaaa
    19021 cagcaaggtc agacaccgca aatgtagtaa ctcagacggg gcaatcgcaa gccaacagga
    19081 taaggacgat tggcgcaact gcgacccttg gcgtatcaat ggggcttgag gctggttttg
    19141 gtgcaatgat gcgcttgtat gagtccaaag cagcaagaaa catgcttctc cgccttgcaa
    19201 acgtcaagcc tggaactccg gcatatgagc gagcgttaaa tcaggctgct aacgccgttc
    19261 gccctctcct aactaacgaa gctaccaggc agtagaaatg aacgccaagg aaggctattt
    19321 aattctcttt tcaatggctg caattattgc ttttcctgat gtttcaggag attttgtagc
    19381 catataagac gaaaaaatca tgtcggtcat tctttcataa cttactattt cccacttagc
    19441 caatgcatgg gacagtttgt agttgtcatc agttagtgcc cttatggaat tttttaagtg
    19501 tttattctct tcagttaatc gcgcaatttt tgtatcaatt tcatgtgagc gatctaattc
    19561 cttaacctgt ttcttgaggg cagctaaccc tgcatatagt acgcaacagg atatcccaag
    19621 agcgagtact actatttcta acacaccaac ctccttagtt tttcgcagga taccagatga
    19681 tactttattg gtggagtggt gtgtgaaaac gtgtcaacga caaaccatcc acaacttgga
    19741 cgaatgattt agcaaaaagt gctatttttg gtgtttggtg tcatagaaaa gtgaatagct
    19801 cacttttcaa cattgcatga aacttgcagg aaatgtgaca ttaccttata ggtaacttcg
    19861 gcgaaaatgc agtaaatgtg aaacgtaatg gtttaaacat gtcgctaaag tggttgtaag
    19921 ttagcctccg aaggattgct gatagctttg ttatagtatt aaatgacatt tgacatagtt
    19981 ggatacttag ctgtggctat caagatggag gtgtgttcat gcttacttgt tttgatgtcg
    20041 ccgactactt cctgtcgcgg tgtgacgagg atagcggtga cacaatctct aatttaaaat
    20101 tacaaaagct tgtttactat gctcagggtt tttcattggc gttgcttggc gagcctcttt
    20161 ttcagaataa gatggaagct tggatgcatg gccctgttgt ccctgagctt tatcgccgtt
    20221 ataaacaata tggtaatggc gctatccctt ctccagagtc ttttgatgct gaaaaattca
    20281 gcgaagaaca gttagagcta ttagaagaag tctgggatgt ttttgggcaa ttttctgcct
    20341 ggaaactgag gaacatgaca cacgaagagt caccatggag atcaaactat attgagggtg
    20401 ttggtggttc tgaaattagc tctaaggaga tggcagagta ctttgcaact cggattaact
    20461 aacgagatgg caagaaaatc taaaaggatt atcccccctg ctgaatctac aggtaagtta
    20521 aagttagggc cgcagtcagg ggggaattca gatcaaaaga aaccgaaatt ttcattctgt
    20581 tacatccaaa gctctcactg cataacgaaa tgccaaaaag atgagaaagc tggcttagct
    20641 gataaattat acagattaag tcaactaaca tgggcagaga ttaagcagca aggtaggcat
    20701 aagcttggat ttgagaagat tgcaaggggt gcaattaaag caggtatccc cagtcatata
    20761 acggaagacg tagatcattt cttggctttt agatttgatg atttgaaagc catggttggt
    20821 tatcgtcttg gatcgacatt ttttgttata tggcttgata gagagttcaa cctatacaag
    20881 cactaataaa acccaccgtc aggtgggttt tttgtacaaa tccttcagcg tatcaaacac
    20941 catcttctta acaagatctg actgctcatc agcgagtcgt tctgcatcgt cacgatatcc
    21001 agtcacaggc gatggttttg atagagcatc ttggacgatt tgtaacaact cggagttcat
    21061 tgatctccca ttcgcctccg ccctgaattt taatttctcc cttacttcca taggcatacg
    21121 gaagttaaag tgcggatcat ctctagccat gccatcactc caagttagtg tattgacatg
    21181 atagaagcac tctactatat tctcaatagg tccacggtgg acctatattg tgaggtgaat
    21241 atgaaaggaa tgagcaagat gccgcagttc aatttgcggt ggcctaaaga agcattggat
    21301 ttggtacgca aggtggcgga agagaatggt cggtctgtta actctgagat ttatcagaga
    21361 gtaatggaaa gctttaagaa ggaagggcgc attggcgcgt aaagtggaag ccccaactgc
    21421 gggaacagtc agggcttcgg ttgtcagtaa atccgtggag aaaaaccaac atgaatagta
    21481 tagcaatttt agaagcagtg aacacccctt acgtaccatt caacggtcag caaattatca
    21541 ccgccatggc tgccggagtt gcatatgttg cgatgaagcc aattgttgaa aaccttggaa
    21601 tgagctggtc aacgcagcaa acaaaactca tgaagcagat tagcaaattc aactgtgttc
    21661 atatgaacat gattgccgct gatgggaagc ttcgtaagct actctgcctt cctttgaaga
    21721 agttaaatgg atggctgttc agcatcaacc ctgagaaagt tcgtgctgac atccgcgata
    21781 aactgattca gtaccaggaa gaatgcttta ctgtgctgca tgactactgg acgaagggaa
    21841 aggcagaaaa tgcacgtaag aaaacatctg ttgatgacag gactccgctt cgtgatgctg
    21901 taaatatgct agtcagcaaa aagcatctaa tgtacccaga agcttatgca atgattcatc
    21961 agcgtttcaa tgtggaaagt attgaagagc ttgatgcatc tcagataccg caagcagtag
    22021 agtacatcca cagggtagtg cttgaaggtg agttcatcgg caaacaagag aagaaagcaa
    22081 acgagctttc tgcaaaagaa gcaaacagcc ttgtatggtt atgggattat gccaaccgtt
    22141 cacaggcatt attccgcgaa ctgtatccgg cattaaaaca aattcaatcg aactattccg
    22201 gcagatgcta cgactacggt catgaattct cgtatgttat cggaatggcg agagacgttt
    22261 taattaatca cacacgagat gttgatatca atgagccaga cggaccaacg aatctttccg
    22321 catggatgag acttaagaat aaagaattac ctccttcagt acataactac tgacagataa
    22381 ccaacgcaac tacccagctt cggctgggtt ttttatgccc aaaattcacc gtagccatgc
    22441 tgcggcgatt ccttgtatct ggagcaaatt aaatgacaga ttcaataaat gccaatgttg
    22501 tggtaagcat gccttcgcaa ctcttcacta tggcgcgttc ttttaaagcc gtagccaatg
    22561 gcaaaattta catcggtaaa attgacactg acccggtaaa tcctgaaaac cagattcagg
    22621 tttatgtaga gaatgaggat ggttctcacg ctcctgtatc gcaaccaatc atcattaacg
    22681 ccgctggata tccggtatat aacggacaga ttgccaagtt cgtaactgtg caaggccatt
    22741 ctatggctgt atatgatgcg tatggtgcgc agcagttcta ttttcctaat gtgctgaagt
    22801 atgacccaga acaatttaaa gatacacttc attctgcgag cggagcggat aatgttggat
    22861 ataggggtta tactgttagt gagtatcttt cacacaccgc attgagtttt ggtaacttaa
    22921 acgattgtct gtctcatgac aaatacaaga taggagataa agtatttgta catggatata
    22981 attatccatc agatggagga atggcttcat ttgaggttgt gtcatcagat gaaccagttg
    23041 gaataaaaac ttacgatggt aagtttaagc taaagcgaac atctgggcat aattatcttg
    23101 agttaataat aaaaaatggt gcgacgccaa agataattgc ccatagagga tgggcaggaa
    23161 taacgccaaa tgaatatcag ggagcattga ctgcgcctgt ttatttcgtt ccagaaaata
    23221 cgagggcatc aattagattt tgtgctgaaa gaggagcttt tggtgttgaa ggggatacca
    23281 aaataacatt agacggagtt cctgtcatat tccatgatga gacagttgat agaactacaa
    23341 atggtactgg tgccgtatca acatttactt ttttagagct aaaggccctc gatgctggat
    23401 cgtatgtttc tggtatttat gcagatgaga aaatactcaa ttatgatgaa tggattagag
    23461 aatgcaagcg tcgcggagta atgccatttg ttgagtggtc tgcgccaatg agtgtcactc
    23521 aagctgataa ttttcttgaa actataaata aatattatgg cacaaagccg acgaatgtct
    23581 ttgtttattc aacatatcct gccgttcttg aactgttgcg ttcaaaaaat gcttatattg
    23641 gacttggtgt tataccatct tacggtgtcg caccaactac agaacaattg gagctggcgt
    23701 ataagcttgg caactgtggt atatctctat caggtggaat aattacgaat gccgctgttg
    23761 taaattcagt taagaaatat ggtttgttgt tgatttatgc aattgctaac accccggcaa
    23821 gaattgataa ttgcatatct gcaggcgttg atataatgat tactgattgc tatagagggt
    23881 aaaatgaata taaaaacata tagacaaatt catggtggca tgaggtcgtt tgttaccaca
    23941 agtttattcc ctgttggggc gaataaattt gacaaatccg atgcaaggaa gtctgttctc
    24001 agtaagacat catcagatag tgcggcgctg gaagtgatgg atttaggatg gctggctgct
    24061 ggtacatgta tttcattttg ctgtgatata aaaataaata ctcaaatgtc tggtgcaccg
    24121 tcaataagca tcgagtcgcg taatggcgca aggctaagtg atggaaggaa aatagtaaac
    24181 ataggcacga ctaactctaa agaatgggag acagtgcgtt gtgattggat tgtgtctgag
    24241 tttgataact ggataactct gtcatatggg gcttctacac ctgtattggg acagtttgag
    24301 ttcagaaatc caagaatttc tatcacgaat gatcaggcta gattgcctat aaatttctct
    24361 ctactcagat caggtaatgt ttggcgtgtt gatactggca catttattta caatggagct
    24421 gtattagtat cagcaaacgg tgactcaata gaggttagtt ggccatatgt gaacaatatt
    24481 agatcaccaa tagttcacac aactatagat actggtttta gtctgttatc tgctggatgt
    24541 atatcatgcg gtccacattc agtaagtaat tcaggatgca aaattcgatt aattaaacct
    24601 gatggctcat ttgtaacaga tgttacaaat ggaacgacac tgagaattca tgtaagcgtt
    24661 aattattaat tcaacatgtg cgcacgtacc cctatgaaca cacgagtctt tgagttgtgt
    24721 gattaatgag acacacaaag ctttgcactt gattgcaagg ctttgtgcta tctgagatat
    24781 agcaatgttg gttgctacac cttttcatca atccaatccg cccaccactg catcatttct
    24841 ctgcgcttat cgagatactg agcatggttg taaatcccgc gcacagatcc gccgctggca
    24901 tgtgccagtt gcacttcaat agcatcagca ggccattcgt tctcgttcat aatcgtgctg
    24961 aattcatgcc tgaatccgtg accgctttcc agaccctcat agccgatttg tttgatcaca
    25021 agtaataccg cgttctcgca gattggcttc ttcttatcgt tgcgcccggc aaaaacaaac
    25081 tctgacactg gtttagtgat tgagcttagc gtagtgagaa gttcaactac ctggtctgac
    25141 ataggaacca catgaatttt gcgtcccttc atcacattgg cgtcgatggt gataatcctg
    25201 ttttcaaaat cgacgttctt ccatagcatg gaacgaagct cttttgttct gagggctgtg
    25261 tagcgtaaaa ctttggtcgc aatgagcgat acgatgcttc ctgaaaatgt tgccagtgct
    25321 ttgttaaatg ccgggatctg gtctgcagga agaaacggga agtttttctt gcggtatccc
    25381 ttcatggcgt cagcaaggtc aggtgccggg ttatatttag ccctaccagt gacaatagcg
    25441 taacggaaaa cctcgccgca tcttctgcgg gctttgttgg ctcgctccat tgcaccgcga
    25501 tcttcaaatc tgcggattac ttcaagaagt tgcatcggct caatatcctg aatttcaagg
    25561 ccgccgatga tgggtaaaat gtcgtcatca aacatttttg caagttcatt tgcatagcct
    25621 actgaccaga cttgcttctt gtgctcgtac cattccttgt aaatggcgct aaaggaattg
    25681 ttgttagacg aagccttttt cgcttttacc ggatcgatgc caaccgagat gtctttcctc
    25741 gcggtccatg ctttatctct tgcctcctgc aaagtcatta gcggatattt tcctacggtc
    25801 aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt ccctgataca
    25861 gggacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc tttcggcttt
    25921 gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggtgggtaa aattatttta
    25981 tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac cattacgaac
    26041 tgtgaatcta cggaaggctt gatattcagg tgattttgcg gactggtacg gatgggagcg
    26101 aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta ggaggggctc
    26161 gttatatcca tttaactaag aggacaatgc ggcatgagta tacccgctaa tggactgcgg
    26221 ggtaagtacg ctgccgctcg attgcttaaa ccctcgccat ttatgccggg tttttatcat
    26281 ttttcttaat gttttccgca cgttctgctt tttggcgtgc ttctgctttg cgcttgttgc
    26341 tcatgtcgtt acggatctgt gcatgactca ttaacgcgaa gataaaggtg ccgccgcaga
    26401 tgttccccgc taaagtaggt agtgcgaagg gccagatgaa atcgctccag tgcagcgtac
    26461 cgttaaacac cagatagagg atttcaacag aaccgacaac gatgtgagtg gtgtcaccca
    26521 gggcaataag ccaggtcatc aatataatca ccacaatctt tgccgcaccc gctgcaggaa
    26581 acatccaaac catagtggcg atcagccagc cggaaatgat cgcgttggca aacatctcgc
    26641 tgggggtgtt cttcatcaca tccatgccga ttttgacaaa tgcatcgcga gtttcttcat
    26701 tgaagattgg catatattca aatgcccacg ccgcaatacc tgtcccgaga atattaccca
    26761 gcagcacgac gccccataac cgcataagta agccaacgtt gctcattgtc ggtttttgca
    26821 tgacgggtag taccgcagtc acggtgtttt cggtaaataa ttgctggcgg gccataatga
    26881 cgataataaa accaaaggta taaccgagat tctccagcaa gaagctgccc ggcacgcctt
    26941 ccagttcgac atgaaatatc ccttttgcca gtagcgaagc gcccatcgac agacccgccg
    27001 caatggctga ccacagtagc gccattgcgt cgcgttccag ctctttttca ccatcctggc
    27061 gaatatgctc atgaattgcc atcgcccggg aggggagtcg gtcttcatct atttctattt
    27121 ttttaccgcg ctctttttct tcgctctcaa cttcaatttc gtcgctgtgt tgatcaattt
    27181 tgtcgttgtc catggtctct tctcttgaat tagcacgtat agctaaagcg tagcggcttt
    27241 tttgctcgca actggcggga gttactctga aaatgtagaa aaggctgcgt ttgccttttt
    27301 ctgtttctat agaatcaagt agcctacagg gcggcgatta ccaggctatg atcaaatcag
    27361 caaatcaggg cgtctggaca tcagttgacg tgctgttaca atcgcccaca cctaaacagg
    27421 cggatacggt atcgttccgt catggatggc aaactgcata agccataaaa aaacagggag
    27481 acatttatga agcttcgcct gtcggcgctt gctctgggaa ctacgcttct ggtggggtgt
    27541 gcgagttccg gtacagatca gcaagggcgt tctgacccgc tagaagggtt caaccgcacc
    27601 atgtacaact tcaacttcaa tgtattagac ccgtatattg ttcgaccggt cgctgtcgcc
    27661 tggcgtgatt atgttccgca accggcgcgt aacggtttga gcaactttac tggcaacctt
    27721 gaagaacctg cggtgatggt taactacttc ttgcagggcg acccttatca ggggatggtc
    27781 cactttaccc gctttttcct gaacaccatt ttggggatgg gcggctttat tgatgttgca
    27841 gggatggcga acccgaaact gcaacggact gaacctcacc gcttcggtag tacgcttggt
    27901 cattatggtg tgggttatgg gccatacgtt cagttaccgt tctacggtag cttcacgctg
    27961 cgtgatgacg gtggtgatat ggcggatggt ctttacccgg ttctttcctg gctgacctgg
    28021 ccgatgtctg tgggtaaatg gacgcttgaa gggatagaaa ctcgtgcgca gttgttggat
    28081 tccgatggtc tgctgcgtca gtcgtccgat ccttatatta tggtgcgcga agcgtacttc
    28141 cagcgtcatg atttcatcgc taatggcggc gaactcaaac cgcaggaaaa tccgaacgcg
    28201 caagcgattc aggatgattt aaaagatatc gattctgaat aagaaacaaa caaaaaaggt
    28261 gagtcgcaaa actcaccttt tttcttatta agcgaacgcg ttatcagaac gcgtagttaa
    28321 agttagtacc gaacagccag gctttacctt cagactcgaa ctggtatggg ccttcgttaa
    28381 ttttcacgct ctgaccgtgc atataagaaa caccaacgtc gactgaagca tctttattaa
    28441 atgcgtaagt cgtacctgca ctcagccaga aacggtcctg gtccggaatg gagatagaac
    28501 gattctgtgc cggaactggg ctgtcatcga aggcgatacc ggtacggaag gtccagttat
    28561 catcgtagta ataagtggta cccaacgcga tgcggtaagc atctttaaag ccttcatgtt
    28621 tctggaacag cgtgtcgcca ctcgttgatg tcgctttcag ttgctggaac tgactccagc
    28681 tggtgtaagc caggctatag tgaattgccc actgcggatc aacacggtta taacccgaca
    28741 cttcccacat ttcaggcagg ttcagcgtca gataaccaga ctgcgttgcg ccacctgtcg
    28801 cggtaggaat tggcaaaccg tagttattaa aagccgggtt aagatcgctg ctgtagttac
    28861 ccttgaagtc aattttcact tcagagcggt aggtcaatgc gtagcggttg tttttatcca
    28921 gttcgtacag aatcccggcg ttccagccaa agccccactg gttaccgttc aggtgagcga
    28981 ttttggtatt actgtcgatg ctgttggcgg tagctgctaa tgcttgcccc tgaggagtca
    29041 gccaagcagg agattgccta atctgaccag ccaccagttg ccccagatcg cctgcgaaac
    29101 gttcaatttt cgcgcgagcg tagacggcgt tgaaaccaag accaaagctc catgcattat
    29161 ttaagcgata tgcaccgctt aagttcaggt tcatggtttc gaggtcggtg gtaccaccga
    29221 cagaaccgcc tgcataagta tcgttaaact ctgtcgccag accatagtta gaggtaattg
    29281 aagcccccca accaaattgg tcgttaatcg gtgcaacaaa gtgcatgttc ggaacccatg
    29341 ccgtaggcgc gatattatcg gctttcaggc tacgaccaga cggagacgtt ccgctgatat
    29401 ttacgtccgg atctatataa accgcacccg cagaaaatgt cgggcggtca aacatggtaa
    29461 tcaacgcggg gttacggctg acgttacctg catcatcggc aatagcgcct tcccctgaat
    29521 aagcccggcc caggccagag gaagaaaatt cgtttaactg aaagcctgcc gaccaggcct
    29581 gggtggagat aagtgccact gcgactgcga gagcagactt tgtaaacagg gttttctggc
    29641 tcatgaccat aacctcaatg atttattttt atacaaacta tgttacgtgc tgtaacagga
    29701 gcgcgaagtg tagggtctga ggtacatctt agaaatcaga ccagtggcga gagtataggt
    29761 cggaccagct ggaatgttgc aagtatgttt ctatcttttt tcaaatatga tctaagaaac
    29821 gcgatccagc tggcaaaatt tggaggtggc gcactcatgg attcgggtga attttgtttt
    29881 agatcatttt taagtgtgat ttcggtcact tatccgattt tgcagaatta acaactggag
    29941 cagcactttc cggaagcgta aaatatccac aacgtttatc tggcaccttg ggtgcacata
    30001 cagaggaaat caacgatgag taaatgtagt gctgatgaaa ccccggtttg ctgctgtatg
    30061 gatgttggca ccattatgga caactccgac tgcaccgcgt cttacagccg cgtgtttgca
    30121 aaccgcgcag aagcagaaca aacgctggcg gcgctgactg aaaaagcccg tagcgtggaa
    30181 tccgaaccgt gcaaaatcac cccgactttc actgaagagt ctgacggtgt gcgcctggat
    30241 atcgatttca cttttgcctg cgaagctgaa atgttgatct tccagctggg tctgcgttaa
    30301 ttcctctcgg gtatatacgc ccctgtcagt tcctggcggg ggcgttttta ttttctcttc
    30361 ctcttttctc tctgtgtcat agctcccact ttcccttttc tccacttggc taaatgtaaa
    30421 aaaatggtta agactatgat caggtcagac cactttattt atttttttac aggggagtgt
    30481 tatgggtcag gttttaccgc tgattacccg ccagggcgat cgtatcgcca ttgttagcgg
    30541 cttacgtacg ccttttgccc gtcaggcgac ggcttttcat ggcattcccg ctgttgattt
    30601 ggggaagatg gtcgtaggcg aattgctggc acgcagcgag atccccgccg aagtgattga
    30661 acaactggtt tttggtcagg tcgtacaaat gcctgaagcc cccaacattg cgcgtgaaat
    30721 tgttcttggc accggaatga atgtgcatac cgatgcttac agcgtcagcc gcgcttgcgc
    30781 taccagtttc caggcgattg caaacgtcgc agaaagcctg atggcgggaa ctattcgcgc
    30841 ggggattgcc ggtggggccg attcctcttc cgtattgcca attggcgtca gtaaaaaact
    30901 ggcgcgcgtg ctggttgatg tcaacaaagc tcgaaccatg agccagcgcc tgaaactatt
    30961 ctctcgcctg cgtttgcgcg acttaatgcc ggtgcctccg gcagtggcgg aatactctac
    31021 tggcttgcgg atgggcgaca ccgctgaaca aatggcgaaa acctacggta ttactcgcga
    31081 acagcaagat gcattagcgc accgttcgca tcagcgggcc gctcaggcgt ggtcagacgg
    31141 taaactcaaa gaagaggtga tgactgcctt tatcccccct tataaacaac cgcttgtcga
    31201 agacaacaat attcgcggta attcctctct tgctgattac gcaaagctgc acccggcgtt
    31261 tgatcgtaaa cacgggacgg taacggcagc aaacagtacg ccgctgaccg atggcgcagc
    31321 ggcggtgatc ctgatgactg aatcgcgggc gaaagaattg ggcctggtgc cgctgggata
    31381 tctgcgtagc tacgcattta ccgctattga tgtctggcag gacatgttgc tcggcccggc
    31441 ctggtcaacg ccgctggcgc tggaacgcgc tggattgacg atggccgatc tgacattgat
    31501 tgatatgcac gaagcttttg ccgcccagac gctggccaat attcagttgc ttggcagcga
    31561 acgttttgcg cgcgacgtac tggggcgtgc acatgccact ggcgaagtgg acgatagcaa
    31621 atttaacgtg cttggcggct cgattgctta cgggcatccc ttcgcggcga ccggcgcgcg
    31681 gatgattacc cagacattgc atgaacttcg ccgtcgcggc ggtggatttg gtttagtgac
    31741 cgcctgtgct gcgggtgggc ttggtgcggc gatggttctg gaggcggaat aatggaaatg
    31801 gcatcagcgt ttacccttaa tgtccgtctg gacaacattg ctgttatcac catcgacgta
    31861 ccgggtgaga aaatgaatac cctgaaggcg gagtttgcct cgcaggtgcg cgccattatt
    31921 aagcaactcc gtgaaaacaa agagttgcgc ggcgtggtgt ttatctccgc gaaaccagac
    31981 aacttcattg ctggcgcaga catcaacatg atcgccaact gcaaaacggc gcaagaagcg
    32041 gaagcactgg cgcggcaggg tcaacagtta atggcggaga ttcatgcttt gcccgttccg
    32101 gttatcgcgg ctattcatgg cgcttgcctg ggcggcgggc tggagttggc gctggcgtgc
    32161 cacggtcgcg tgtgtactga tgatcctaaa acggtgctcg gtttgcctga agtacaactt
    32221 ggattgttac ccggttcagg cggcacccag cgtttaccgc gcctgatagg tgtcagcaca
    32281 gcattagaga tgatcctcac cggaaaacaa cttcgggcga aacaggcagt aaagctgggg
    32341 ctggtggatg acgttgttcc acactccatt ttgctggaag ccgctgttga gctggcaaag
    32401 caggatcgtc catcttcacg ccctctacct gtacgcgagc gtattctggc ggggccgtta
    32461 ggtcgtgcgc tgctgttcaa aatggtcggc aagaaaacag aacacaaaac tcagggtaac
    32521 tatccggcga cagaacgcat tcttgaagtt atcgaaaacg gattagcgca aggcaccagt
    32581 agcggctatg acgctgaagc tcgggcgttt ggtgaactgg cgatgacgcc gcaatcgcag
    32641 gcgctgcgta gtatcttttt tgccagtacg gacgtgaaga aagatcctgg cagtgatgcg
    32701 cagcctgcgc cattaaacag cgtgggaatt ttaggtggtg gcttgatggg cggcggtatt
    32761 gcttatgtca ctgcttgtaa agcggggctt ccggtcagaa ttaaagatat caacccgcag
    32821 ggcataaatc atgcgctgaa gtacagttgg gatcagctgg agggcaaagt tcgccgtcgt
    32881 catctcaaag ccagcgaacg tgacagacag ctggcattaa tctccggaac gacggactat
    32941 cgcggctttg cccatcgcga tctgattatt gaagcggtgt ttgaaaatct cgaattaaaa
    33001 caacaaatgg tggcggaagt tgagcaaaat tgcgccgctc ataccatctt tgcttcgaac
    33061 acgtcatctt taccgattgg cgatatcgcc gctcacgccg cgcgacctga gcaagttatt
    33121 ggcctgcatt tcttcagtcc ggtggaaaaa atgccgttgg tagaaattat tccccatgcc
    33181 ggtacatcgg cgcaaaccat cgccaccaca gtaaaactgg cgaaaaaaca gggcaaaacg
    33241 ccaattgtcg tgcgtgacaa agccgggttt tacgtcaatc gcatcttagc gccttacatt
    33301 aatgaagcta tccgcatgtt gaccgaaggt gaacgggtag agcacattga taccgcgctg
    33361 gtgaaatttg gttttccggt aggcccaatc caacttttgg atgaggtagg aatcgacacc
    33421 gggactaaaa ttattcctgt actggaagcc gcttatggag aacgttttag cgcgcctgca
    33481 aatgttgttt cttcaatttt gaacgacgat cgcaaaggca gaaaaaatgg ccggggtttc
    33541 tatctttatg gtcagaaagg gcgtaaaagc aaaaaacagg tcgatcccgc catttacccg
    33601 ctcattggca cacaagggca ggggcgactc tccgcaccgc aggtagctga acggtgtgtg
    33661 atgttgatgc tgaatgaagc agtacgttgt ctggacgagc aggttatccg tagcgtgcgt
    33721 gacggagata ttggcgcggt atttggcatt ggttttccgc catttctcgg tggtccgttc
    33781 cgctacatcg attctctcgg tgcgggtgaa gtggttgcaa taatgcaacg actggccacg
    33841 cagtatggtt cccgttttac cccttgcgag cgtttagtcg agatgagcaa gcgtggggaa
    33901 agtttttgga aaacaactgc aactgacctg caataagaag gtcaaagcta tatgaatccg
    33961 cgctgaatgg cggagtgttg ctcaaaatgt aaacgcatat tgactatact tacgccattg
    34021 aggtaaaaaa cagcgtttca ttcggtgaat ggataaggca caatgccggc caccgttttc
    34081 tttctctggt ttcagatgaa agaaaacggg cgaatctggt taacaaaagc ggtgcaatat
    34141 gcaagttttt atcatgcgtc acggcgacgc agccctcgat gccgccagtg attcggttcg
    34201 tcctctgact actaacggtt gtgacgaatc tcgcctgatg gcgaactggc tgaaaggtca
    34261 aaaagtggaa atcgaacgcg ttctggtgag cccgttcctg cgagccgagc aaacactgga
    34321 agaagtaggg gattgtctga acctgccttc cagtgcagaa gttctgccgg agttaacgcc
    34381 ctgcggcgat gttggtctgg tcagtgccta tttgcaggcg ttgaccaatg aaggtgttgc
    34441 ttcggtactg gtgatctccc acttaccctt agtcgggtat ctggttgctg agttatgtcc
    34501 gggcgaaacg ccgcctatgt ttaccacctc ggccattgcc agcgtaacgc ttgatgaaag
    34561 cggaaacggc acatttaact ggcagatgag cccatgtaat ttgaagatgg caaaggcgat
    34621 ttaatacgca tgcacaaggc ccgctggcgt aagctatcgg gcctttattt tttctggcaa
    34681 tgttgaattt attcaatctt attgtactaa caatgatccg ct
//
LOCUS       DAPHCV010000048        34582 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.048, whole genome shotgun sequence.
ACCESSION   DAPHCV010000048 DAPHCV010000000
VERSION     DAPHCV010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.048"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            27..767
                     /locus_tag="RH033_002275"
     CDS             27..767
                     /locus_tag="RH033_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDV2991451.1"
                     /translation="MFFNTKHTAALCLASCMAFSSSAIADIVISGTRVVYKSEQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTASS
                     ALPKDRESVFWFNVLEVPPKPDAAKAANQSLLQLAFRTRIKLFYRPEGLSGIPSDAPL
                     ALKWSWATSEGKAALRVDNPTPYYVSFSSGDLEANGKRYPLDVKMIAPFSNDVIKVNG
                     MSGRTSSAKVHFFAINDFGGSIEGNANL"
     gene            802..3402
                     /locus_tag="RH033_002276"
     CDS             802..3402
                     /locus_tag="RH033_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HDV2991452.1"
                     /translation="MTIKSTKKYHNHLTRIATFCALLYSNSALCAELVEYDHTFLMGK
                     DASNIDLSRYTEGNPTLPGIYDVSVYVNDQPIMSQSIAFTVIEGKKNAQACITQKNLL
                     QFHISSPDKNSEKAILLKRDEDLGDCLNLAEMIPQSSIRYDVNDQRLDIDVPQAWIMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSESPGRTNDSIYAAFNGGINLGAWRLRASG
                     NYNWMTNVHSDYDFQNRYLQRDLASLRSQLVIGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPVLASFAPIIHGVANTNAKVTVMQNGYKIYETTVPPGAFAIDDLSPSGYGSDLIVT
                     IEEADGTKRTFSQPFSSVVQMLRPGVGRWDISAGQVLKDSIQDEPNLFQASYYYGLNN
                     YLTGYTGIQLTDNNYTAGLLGLGMNTPVGAFSVDVTHSNVSIPDDKTYQGQSYRISWN
                     KLFENTSTSLNIAAYRYSTQHYLGLNDALTLIDEVEHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNSTNYSIGYSNSASWGSYSISAQRSLN
                     EDGQTDDSIYLSFTIPIENLLGTEHRSSGFQSIDTQLNSDFKGNNQLNISSSGYSDTN
                     RISYSVNTGYMMNKSSDDLSYIGGYASYESPWGTLSGSASASSDNSRQFSLNTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVIQAPGAKGARINYGNSTVDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSTVAVPRQGAVVFADFETVQGQSAIMNIVRSDGKNIPFAADI
                     YDEQNNIIGNVGQGGQAFVRGIGQEGNIRITWIEEGKPVSCFAHYQQNTTSEKIAQSI
                     ILNGLRCQIQ"
     gene            3419..3991
                     /locus_tag="RH033_002277"
     CDS             3419..3991
                     /locus_tag="RH033_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2991453.1"
                     /translation="MIKTTPHKIAILMGLLLSPSVFATDVNVDFTATVKATTCNITLT
                     SLNGSNVVDNGNDSYTLIIPKMGLDKIVNKASQSEANFKLVASGCSSGISWIDTTMTG
                     NQSGSTSLIIPQSSDSSSTTSNIGMGFKRLETSGDTFLKPNSAEYIRWKTTEISTSGL
                     EMTVALRETTPGQGIPGKFRAQATFNFIYQ"
     gene            4003..4611
                     /gene="yadL"
                     /locus_tag="RH033_002278"
     CDS             4003..4611
                     /gene="yadL"
                     /locus_tag="RH033_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020235143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HDV2991454.1"
                     /translation="MMILKKLCYGLSGSLFLASSLLTISSHAATDSIGLTVTTDIEMG
                     TCTSTLTDDGTAKLSTINFGDVYISEINAETKIKTFKLQFKDCAGIPGKKAKIKLTPR
                     ALCEGNSNNGPGFANASTATAKAAAVAVEVWSTSTPGKNGAKQFSCVTPATEEVSIAG
                     ATGGDVVDYPMSAVLVVAKDKTVTDVTAGDFTAPATFTVTYN"
     gene            4638..5234
                     /locus_tag="RH033_002279"
     CDS             4638..5234
                     /locus_tag="RH033_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2991455.1"
                     /translation="MRPTQRNLMKKSILLLSLLFSTTFPVVAGQDVDIVANIKNNTCQ
                     SGISNNGNIDLGVVGVGDFTGNISAENYHPGGKEFTITVQDCTLQGTGNVLNQLHIDF
                     RALSGVMATGSSQIFANEDNTGAKNVGVVIFSIQDPANTFNVLGVTGSSRSVYPVMSS
                     ALNNSSWKFSTRMQKIDPALNVTSGQLISHVLVDIYYE"
     gene            5284..6549
                     /locus_tag="RH033_002280"
     CDS             5284..6549
                     /locus_tag="RH033_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HDV2991456.1"
                     /translation="MKIFFRYFLFLALCSCCYTASAGTDDNVSYLVGNDYGVGPGDQK
                     WRETGPSADITVKFRYSTSTNNLVFYKPTQLGPTGVSLYWSQLNSASGGGYLYCNRSN
                     TASGEPMAIENQMVDSGKRYGGHKLFETSVPGLYYTLKISNIWSSYTTTNISSSGMYI
                     GDSPTQYFRWIPESEATLYWSCNNANSSNKYWAVGGVWQSLTVEFYTDTNFDPTKTNK
                     VTLSKPGGYLYSFKGYGLGGGINDHSYFLKIDFDLDNMTLSNPTCFTAALTGPSVSGS
                     TVKMGDYTPAQIKNGATAVPFDITLQNCIRVRNIETKLMSNKVGSVSKELLANTLTGN
                     NAAKGVGVLIEGLKNTKSAQMVLKPNDSTSVYKDYETEDDTTGGIYPDKGNGTSQPLH
                     FQATLKQDGNIAIEPGDFKATSTFQVTYP"
     gene            6661..7455
                     /gene="panB"
                     /locus_tag="RH033_002281"
     CDS             6661..7455
                     /gene="panB"
                     /locus_tag="RH033_002281"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDV2991457.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFAEEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVQMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPIELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            7467..8318
                     /gene="panC"
                     /locus_tag="RH033_002282"
     CDS             7467..8318
                     /gene="panC"
                     /locus_tag="RH033_002282"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDV2991458.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIIGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLQEVSENSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(8698..9618)
                     /locus_tag="RH033_002283"
     CDS             complement(8698..9618)
                     /locus_tag="RH033_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDV2991459.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLDIHLPAELRELCDLDTL
                     HLESGSFIEESLKGHSMDVLYSVQMQGNPGYLHVVIEHQSKPDKKMTFRMMRYAIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNNPFPLVDITI
                     TPDDEIMQHRRIVILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGQSMMTLAQWFEERGRQEERQEVRQEVIQEVRQEVRQEF
                     ALRFLSKGMSREDVAEMANLPLAEVDKLIS"
     gene            9892..10272
                     /gene="panD"
                     /locus_tag="RH033_002284"
     CDS             9892..10272
                     /gene="panD"
                     /locus_tag="RH033_002284"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HDV2991460.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10276..11505)
                     /locus_tag="RH033_002285"
     CDS             complement(10276..11505)
                     /locus_tag="RH033_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDV2991461.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYTTLSMVQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     DPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFSRSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(11569..12009)
                     /locus_tag="RH033_002286"
     CDS             complement(11569..12009)
                     /locus_tag="RH033_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDV2991462.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMLA
                     YRETLSSAEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12114..12884)
                     /locus_tag="RH033_002287"
     CDS             complement(12114..12884)
                     /locus_tag="RH033_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2991463.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(12881..13807)
                     /locus_tag="RH033_002288"
     CDS             complement(12881..13807)
                     /locus_tag="RH033_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV2991464.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            13916..14578
                     /gene="can"
                     /locus_tag="RH033_002289"
     CDS             13916..14578
                     /gene="can"
                     /locus_tag="RH033_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HDV2991465.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(14619..15155)
                     /gene="hpt"
                     /locus_tag="RH033_002290"
     CDS             complement(14619..15155)
                     /gene="hpt"
                     /locus_tag="RH033_002290"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDV2991466.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15361..17751
                     /gene="gcd"
                     /locus_tag="RH033_002291"
     CDS             15361..17751
                     /gene="gcd"
                     /locus_tag="RH033_002291"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HDV2991467.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILAWAGFNDPQEISGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKNLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(17947..19497)
                     /gene="cueO"
                     /locus_tag="RH033_002292"
     CDS             complement(17947..19497)
                     /gene="cueO"
                     /locus_tag="RH033_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HDV2991468.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNNPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPATHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            19663..20010
                     /locus_tag="RH033_002293"
     CDS             19663..20010
                     /locus_tag="RH033_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HDV2991469.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20116..20982
                     /gene="speE"
                     /locus_tag="RH033_002294"
     CDS             20116..20982
                     /gene="speE"
                     /locus_tag="RH033_002294"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HDV2991470.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            20998..21792
                     /gene="speD"
                     /locus_tag="RH033_002295"
     CDS             20998..21792
                     /gene="speD"
                     /locus_tag="RH033_002295"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HDV2991471.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTTEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSEDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(21830..22192)
                     /locus_tag="RH033_002296"
     CDS             complement(21830..22192)
                     /locus_tag="RH033_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HDV2991472.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQWAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22367..24964)
                     /gene="acnB"
                     /locus_tag="RH033_002297"
     CDS             complement(22367..24964)
                     /gene="acnB"
                     /locus_tag="RH033_002297"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HDV2991473.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            25319..27076
                     /locus_tag="RH033_002298"
     CDS             25319..27076
                     /locus_tag="RH033_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HDV2991474.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     LAAPDISAVTLPATVSVPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQEVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVSTANPVITEPATTVISIEPTNPDVVYIPNYKPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHATPVITRDTQRQAAAQRFNEAENYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRVQSASPEQRQVFREKVQESRPQRLNDSNRTARLNNE
                     QRSAVRERLSERGARRLER"
     gene            complement(27147..28571)
                     /gene="lpdA"
                     /locus_tag="RH033_002299"
     CDS             complement(27147..28571)
                     /gene="lpdA"
                     /locus_tag="RH033_002299"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDV2991475.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYATLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(28896..30788)
                     /gene="aceF"
                     /locus_tag="RH033_002300"
     CDS             complement(28896..30788)
                     /gene="aceF"
                     /locus_tag="RH033_002300"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDV2991476.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPVKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(30803..33466)
                     /gene="aceE"
                     /locus_tag="RH033_002301"
     CDS             complement(30803..33466)
                     /gene="aceE"
                     /locus_tag="RH033_002301"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HDV2991477.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(33627..34391)
                     /gene="pdhR"
                     /locus_tag="RH033_002302"
     CDS             complement(33627..34391)
                     /gene="pdhR"
                     /locus_tag="RH033_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HDV2991478.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         8681 a         9097 c         8322 g         8482 t
ORIGIN      
        1 ttaattaact ctgtgaggaa cggtttatgt tttttaatac taaacatacc gcagcattgt
       61 gcttagcgag ctgtatggct tttagctcat cagctattgc agacatcgta atttcaggta
      121 ctcgcgtagt ttataaaagc gaacaaaaaa gcgtcaatgt gcgtctggaa aataaaggca
      181 ataacccatt gctggttcag agttggttag atactggaga tgacaacgct gaaccaggca
      241 gtattactgt cccctttacc gctacgccac cagtatcacg tattgatgct aaacgtggcc
      301 aaacaatcaa actgatgtac accgccagta gtgctctgcc aaaagacaga gaaagtgtgt
      361 tctggtttaa cgtactggaa gttccaccaa aacccgatgc agcaaaagca gcaaatcaaa
      421 gcctgctaca actggcattt cgtacacgta taaaactgtt ctatcgcccg gaaggattga
      481 gtggaattcc atctgatgcg ccattggctc tgaagtggtc ctgggcaaca tctgaaggca
      541 aagcggcatt acgcgttgat aaccctacgc cctactatgt ctcttttagc agtggtgatt
      601 tagaagccaa cggcaaacgc tatcccctgg atgtgaaaat gattgcgccg ttcagtaacg
      661 acgtcatcaa ggtaaatggg atgagtggca gaacaagctc tgcaaaagtg cattttttcg
      721 ccattaatga ttttggcggc tctattgaag gcaacgccaa cctttaatct ggcaaggata
      781 taccacagca ggaagcagaa cgtgactatt aaatccacta aaaaatatca taatcacctt
      841 acccgcatcg ccacgttttg cgcgctgctg tatagcaaca gtgctttatg cgctgaactc
      901 gtcgaatatg accatacatt tctgatggga aaggatgctt caaatatcga ccttagtcgg
      961 tatacagaag gcaacccgac actaccaggc atttacgatg tcagtgtgta tgttaacgat
     1021 caacctatca tgagccaaag cattgctttt actgttattg aaggtaaaaa gaacgctcag
     1081 gcctgcatta cgcagaaaaa tttattgcaa tttcatatca gttcaccaga caagaatagc
     1141 gaaaaagcca ttttgcttaa gcgagacgaa gatcttggtg attgcctcaa tctggctgaa
     1201 atgatccctc aatcatccat tcgttacgat gttaacgatc aacgcctgga tatcgacgtc
     1261 ccccaggcat ggattatgaa gaattatcaa aactatgttg atccctcttt gtgggaaaat
     1321 ggcattaatg cagccatgct ttcctacaac ctcaatggat atcacagtga aagtccaggc
     1381 agaacaaatg acagtatcta tgcggcattc aacggtggga taaacttagg cgcgtggcgg
     1441 ttacgtgcct caggtaacta caactggatg accaatgtcc atagtgatta tgattttcag
     1501 aatcgctatt tacaacgcga tcttgcgtca ctacgttcac aactggttat tggtgaatct
     1561 tacacgaccg gcgagacctt cgattcagtc agcattcgcg gtattcgctt atacagtgac
     1621 agtcgaatgc tgcctccggt attagccagt tttgcgccaa tcattcatgg cgtagcgaat
     1681 accaatgcca aagtaaccgt tatgcaaaat ggttataaaa tctatgaaac gacggtgccg
     1741 ccaggggcct ttgccattga tgatcttagc ccgtcaggtt atggcagtga tctcattgtg
     1801 actatcgaag aagctgatgg cacaaaacgg acgttctccc agccattttc atctgttgtg
     1861 caaatgctgc gccctggcgt tggtcgctgg gatattagcg ccggtcaggt tttaaaagac
     1921 agtatccagg atgagcctaa tttatttcaa gccagctact actatggttt gaataactac
     1981 ctgacaggat ataccggtat tcagctaact gataataact ataccgccgg attgctgggg
     2041 cttggtatga atacccccgt gggggctttt tctgtcgatg ttactcactc aaatgtcagc
     2101 attccggacg ataaaaccta tcaaggacaa agctaccgta tttcctggaa caagttattt
     2161 gaaaatacca gtacatcatt aaatatcgcg gcctatcgct actccaccca gcattatctg
     2221 ggcctcaatg atgcgctgac gctgattgat gaagtagaac atcctgagca agatcttgag
     2281 cctaaatcca tgcgcaatta ctcgcgcatg aaaaatcagg tcacggtcag tatcaaccaa
     2341 ccgctgaaat ttgagaaaaa ggattacggt tcattttatc tctcgggaag ctggtcagat
     2401 tactgggcct ccggacaaaa cagtactaat tactctattg gttatagcaa tagtgcgtcc
     2461 tggggcagtt acagtatcag tgctcaacgt tcgttgaatg aagatggtca aaccgacgat
     2521 agcatttatc tcagcttcac gattccaata gaaaatttac tgggtacgga acaccgcagc
     2581 tccggcttcc agagtattga tacacaatta aacagcgact ttaagggtaa taaccaactc
     2641 aatatcagca gtagtggtta cagcgatacc aatcgcatca gctacagcgt aaacacgggt
     2701 tatatgatga ataaatccag tgatgacctg agttatatcg gcggttatgc cagttatgaa
     2761 tcgccgtggg gaactctgtc aggttcagcc tcagcaagca gtgataatag tcgccaattc
     2821 tcactcaata ctgatggtgg atttgtgtta catagcggcg ggctgacgtt cagtaatgac
     2881 agttttagtg attccgacac tctggctgtc attcaggcgc caggagcaaa aggcgctcgt
     2941 attaactacg gaaacagcac cgtagaccgc tggggttatg gtgtgaccag cgccctttcg
     3001 ccataccatg aaaaccgtat cgcactggac attaacgatc tggaaaacga tgttgaattg
     3061 aaaagcacca gtactgtcgc cgtcccgcgc cagggagccg tcgtctttgc tgattttgaa
     3121 acagttcagg gacaatcggc aattatgaat atcgtgcgaa gcgatggtaa aaatattcca
     3181 tttgcagcgg atatttatga tgaacaaaac aatatcatcg gcaacgtagg tcagggtggt
     3241 caggcctttg ttcgcggtat cgggcaggaa ggaaatatac gaattacctg gattgaagag
     3301 ggcaaaccag tcagttgttt tgctcattac caacaaaaca cgacatcaga aaaaatagcg
     3361 caatctatta ttctgaacgg acttcgttgt cagatacagt aaccacaagg gacttccaat
     3421 gataaaaacc acaccacata aaatagcaat actcatgggg ctattattat cgccatctgt
     3481 atttgcaacg gatgttaatg tcgattttac cgctaccgtg aaagcgacaa catgtaacat
     3541 caccctgaca tcgctgaatg gttcaaacgt cgttgataat ggcaatgata gttataccct
     3601 gataatcccc aagatgggac tggataagat agtcaataaa gcgtcacaat ctgaggccaa
     3661 cttcaaatta gtcgccagtg ggtgtagcag tggaattagc tggattgata ccacaatgac
     3721 tggaaatcag tctggtagta catcgctgat tattccacaa tcttctgact cctcctcaac
     3781 aaccagtaat attggtatgg gatttaaaag attagagact tcgggcgata cgtttctgaa
     3841 acccaatagc gcggaataca tccgctggaa aaccacagaa attagcacca gtggtctgga
     3901 aatgactgtc gcactgcgcg aaacaacccc aggacagggc atacccggga aatttcgcgc
     3961 acaggcaacg ttcaatttca tctatcaata acaaggatga caatgatgat attaaaaaaa
     4021 ttatgttatg gattatcggg tagtctattt ctggcttcat cactgttaac catttcctca
     4081 catgcggcca ctgatagtat tggcctgact gttactacag acatagaaat gggtacctgt
     4141 acatcaacgt tgactgatga cggtacagct aaactgtcca ccatcaattt cggtgatgta
     4201 tatatttctg aaattaatgc cgaaactaaa attaaaacct tcaaattgca gttcaaagac
     4261 tgtgcgggga ttcccgggaa aaaggccaaa ataaagttga ccccacgagc cttatgcgaa
     4321 ggtaattcta ataacggccc tggatttgct aacgcatcaa cggcaacagc aaaagcagcg
     4381 gcagtcgccg ttgaggtctg gagtacgtcc acaccaggta aaaacggcgc aaaacaattc
     4441 agttgcgtca cccccgcaac agaagaagtc agtattgctg gtgcgacggg aggtgatgtt
     4501 gtcgattacc cgatgagtgc tgttttggtg gtcgcgaaag ataaaaccgt tacagatgtc
     4561 accgcgggtg atttcaccgc tccggcaaca tttactgtga cttataacta acaggcagac
     4621 cgattcagga actaaatatg cgccccactc aacgtaatct gatgaaaaaa tcaattctgc
     4681 ttctgtcttt gctattcagc accactttcc ctgtggttgc aggacaggat gttgacattg
     4741 ttgcaaatat aaaaaataac acctgccaaa gtggaatcag caacaacgga aatattgatt
     4801 taggtgtcgt tggcgtgggc gattttaccg gaaacatttc agccgaaaat tatcaccccg
     4861 gtggaaaaga gttcactatt acagtacagg actgcacact tcagggcaca gggaatgtgt
     4921 taaatcagtt acatattgat tttcgcgcac tcagtggcgt aatggcgaca ggttccagtc
     4981 aaatattcgc caatgaagac aacaccgggg ctaaaaatgt tggggtagtt atattctcta
     5041 ttcaggaccc ggcaaatacc ttcaacgttc ttggcgtaac tggtagctca cgttccgttt
     5101 atccggtcat gtcatcagca ctaaacaatt catcctggaa atttagcact cgaatgcaaa
     5161 aaatcgatcc agcgctgaat gtcacctctg gccaacttat aagccatgtg ttagtggata
     5221 tttactacga ataacagaca aagtgctgca agcattatct tgcagcatga caatggagta
     5281 aaaatgaaga tattcttcag atactttctt tttttagcac tatgctcttg ttgttataca
     5341 gcaagtgccg gcaccgatga taacgtcagc tatcttgttg ggaatgatta cggagtgggt
     5401 ccgggagacc aaaagtggag agaaacaggc cccagtgcag acattactgt caaattcagg
     5461 tattcaacaa gcacaaataa cttagttttt tataaaccaa cccaactcgg accaacaggt
     5521 gtgtcattat actggtctca gttaaatagc gcttcaggtg gaggttacct ttactgcaac
     5581 agaagtaaca ctgcaagcgg tgagcctatg gcaattgaaa atcaaatggt tgattcgggt
     5641 aaacgttatg gcgggcataa actatttgaa acatctgtcc caggcctgta ttatacatta
     5701 aagatatcaa acatttggtc atcatacacc acaaccaata tcagttcatc aggtatgtat
     5761 atcggcgatt ctccaactca atattttcgg tggatccccg aaagtgaagc cactctatat
     5821 tggtcttgta acaatgcaaa ctcatctaat aaatactggg ccgtaggtgg agtttggcag
     5881 tcgctcacgg tggaatttta taccgatacc aactttgacc ccacaaagac aaacaaggta
     5941 acattatcta agccgggtgg ttatctctac tcattcaaag gatatggtct tggtggcggt
     6001 atcaacgatc acagctattt ccttaaaata gattttgacc tggataatat gacgttatcc
     6061 aacccaacgt gttttactgc tgcacttact ggcccatctg taagcggttc aacagttaaa
     6121 atgggagatt ataccccagc gcaaataaaa aatggcgcaa cagcggttcc ttttgatatc
     6181 acgctacaga actgtattcg tgttcgcaat atagaaacaa aacttatgtc aaacaaggtg
     6241 ggcagtgtaa gcaaagaact tctggctaac acactcacag gaaataatgc agcaaaaggc
     6301 gttggtgtac tgattgaagg tttaaaaaac actaaaagtg cacaaatggt actgaaacca
     6361 aatgattcaa cgtctgttta taaagattac gaaacagaag atgacactac cggaggaatt
     6421 tacccggata aaggaaacgg tacctcacaa cctcttcatt tccaggctac attaaaacaa
     6481 gacggtaata ttgctataga accgggggat tttaaagcca ccagcacttt tcaggttacc
     6541 tacccctgat aacactatcg caccgccaga caacggcggt gcgatacgga taaatcattg
     6601 cccccaccaa tgtgactgat aaaatgcgag acagcacaac atcatttatc aggatacgct
     6661 atgaaaccaa ccacgattgc ttcattgcag aaatgcaagc aggacaaaaa acgtttcgca
     6721 accatcaccg cttacgacta tagcttcgca aaattgtttg ccgaagaagg acttaacgtc
     6781 atgctggtag gcgattcact gggcatgacg gttcaggggc atgactccac gctaccggtt
     6841 accgttgccg atatcgccta tcacaccgcc gctgtgcgtc gcggcgcgcc aaactgcctt
     6901 ctgctggctg acctgccatt tatggcgtat gccacgccgg aacaagcttt tgaaaacgcc
     6961 gcaacggtaa tgcgtgccgg agccaacatg gtcaaaattg aaggcggtga gtggctggtg
     7021 gaaaccgtac aaatgctgac cgaacgtgcc gttccagttt gtggtcattt aggcctaacc
     7081 ccacagtcag tgaatatttt cggtggctac aaagtccagg ggcgcggcga tgaagcgggc
     7141 gatcaactgc tcagcgatgc attggcatta gaagctgctg gggcacagct gctggtgctg
     7201 gaatgcgtgc cgattgaact ggcaaaacgt attaccgaag cactggcgat cccggttatt
     7261 ggcattggcg caggcaacgt cactgacggg cagatcctcg tgatgcacga cgcgttcggc
     7321 attaccggcg gccacattcc taaattcgct aaaaatttcc tcgccgaaac gggcgacatc
     7381 cgcgcggctg tgcggcagta tatggctgaa gtggagtccg gcgtttaccc gggcgaagaa
     7441 cacagtttcc attaaggagt gacgttgtgt taattatcga aaccctgccg ctgctgcgtc
     7501 agcaaattcg ccgcctgcgt atggaaggta agcgcgtggc gctggtgccc accatgggta
     7561 acctgcacga tggccatatg aagctggtcg acgaagccaa agcccgcgcc gatgtggtag
     7621 tggtcagtat tttcgttaac ccgatgcagt ttgaccgccc ggaagatctg gcacgttatc
     7681 cacggacctt gcaagaggac tgcgaaaagc tgaacaaacg taaagtggat ctagttttcg
     7741 cgccttcggt aaaagagatc tacccgaacg gtacagaaac ccacacctat gttgatgttc
     7801 ccggtctttc caccatgctg gaaggtgcca gccgtccggg acattttcgc ggcgtttcga
     7861 ctattgtcag caagctgttc aatctggtcc agccggacat cgcctgcttc ggtgaaaaag
     7921 attttcagca actggcgctg atccgcaaaa tggttgctga tatgggcttt gatattgaga
     7981 ttatcggtgt gccaattatg cgcgccaaag acggtctggc gctgagttcc cgtaacggtt
     8041 atctgacggc ggaacaacgc aaaattgcgc ccggtctgta caaagtttta agttcaattg
     8101 ccgacaaatt gcaggctggg gaacgggatc tcgatgaaat tattgctatt gccgggcaag
     8161 aactgaatga aaaaggcttc cgcgccgatg atattcagat tcgtgatgcc gacacgttgc
     8221 aggaagtctc tgaaaacagc aaacgggcag tgattctggt agccgcctgg cttggcgatg
     8281 ctcgcctgat cgacaacaaa atcgtcgaac tggcgtaatc cttatctggc gctacggctg
     8341 atggcgccag ttattaattt accccacgtc atggcgatga cgtctgtaag cgattattca
     8401 taacgcagac aacttagtta gagttaatta gcttatcaac ctctgtaaga gataaagttg
     8461 ccatctctgc aacgtcttcc cgagacattc ctttactcag aagacgctgg gccagcctta
     8521 ccttccggag tccctcttta tatcccttct gaaatccctt ctgaaatccc ttctcttcaa
     8581 accactgcgc caacgtctgc ctggtttctc tccgtgtctt tatttccata tgttccctta
     8641 attatctggt cagttaacaa taacaattat ctggctataa ctgataatgc agataactta
     8701 gctaatcagc ttatcaacct cagcaagagg taaatttgcc atctctgcaa cgtcttcccg
     8761 agacatccct ttactcagaa aacgcagggc gaattcctgt cttacttcct gtctaacctc
     8821 ttgtattacc tcctgtctta cctcctgcct ttcctcctgt cttcccctct cttcaaacca
     8881 ctgcgccagc gtcatcatag actgccctcc cgtttccctg tctctcagca caccataaaa
     8941 caaatccgct tgttcagtat gaccgcgttg cagcatatag ttttgcatgg caactaactg
     9001 acttccgcta gtgtaccctt cgtcaatcag cgtgaccagt tgctccagca ataacattaa
     9061 gtcgcgctgg cgaatatgtt tttgcagtag ttcgagaatc acaatccgcc gatgttgcat
     9121 gatttcgtca tccggtgtga tggtgatatc caccagcggg aaagggttgt tgtaaacgcg
     9181 tcgcgccagc tccggcgagt aaaacatatc aaaccagcac attgagagcg gataaggtgt
     9241 ggcctcgccc tgataaaaca gaatcggcac caccagcggc agcttatcgt gatccgcctc
     9301 cagatgccgg tgcatggcgg caatggcata gcgcatcatg cgaaaggtca ttttcttatc
     9361 cggcttgctt tgatgttcaa tcaccacatg caaataaccg ggattaccct gcatttgcac
     9421 ggaatagagt acgtccatgc tgtgcccttt caggctttct tcaataaaac tccccgactc
     9481 aagatgcagc gtgtcgaggt cacacagttc gcgtagttcc gctggcaaat ggatatccag
     9541 aaagtcacga gccgtttccg catgcattaa aaattgttta aataccgcgt catgcggtgt
     9601 ggtactcggt gcatccatct tccctgcctt ccgtgttgtc ttgcgtgaag gcagtatcca
     9661 gtcaaggcgc gccgtcgtca gccgcctctt tagtgtttac cgagcagcgt tcagagaaat
     9721 ttcaattggt tacaggcgtt acatggcacg cttcggcacg gtacgcaaac tgctgatgca
     9781 aatgccgtca gatgagtaga cactaaacaa aaatcgggca atactgcgtg agaatttcct
     9841 caaagcaggc atcgcctgct tcgttaacga cagggtagaa aggtagaagt tatgattcgc
     9901 acgatgctgc agggcaaact ccaccgcgtg aaagtgactc atgcggacct gcactatgaa
     9961 ggttcttgcg ccattgacca ggattttctt gacgcagccg gtattctcga aaacgaagcc
    10021 attgatatct ggaatgtgac caacggcaag cgtttctcca cctatgccat cgcggcagaa
    10081 cgcggttcga gaattatttc cgttaacggt gcggcggccc actgcgccag cgtcggcgat
    10141 attgtcatca tcgccagctt cgttactatg ccagatgaag aagctcgcac ctggcgaccc
    10201 aacgtcgcct attttgaagg cgacaatgaa atgaaacgta ccgcgaaagc cattccggta
    10261 caggttgctt gattgttatc cctgcggctg gttactcacc agccgcgaca tcgtctccag
    10321 cgaatccgtt cttaagatat aaagtcgttt tagtaacaac ggattatctc ccggttttac
    10381 tttgcctttc atagtcgtca ccgccaggtg aaatccggca tcgtttgctg ccttcacggc
    10441 tttgtcatta aagccgccaa acggatacga aagataccag acatgcggat taaattgcgc
    10501 cagagcgcgg cgtgaacgtg aaaaatcaaa cagaatatta tgctcactac ggctcagtaa
    10561 tatgggtcgg cgataaccat ccacacgatg caaaaaatgg gtatgtgact ggaaatcaaa
    10621 tacatcgcga atttcgttaa gttcagaaac gctcataaat tgcagcgatt ttgggtccca
    10681 cttctgcggg tgacgtttga tgcgcgaggt aacaataaac gccgtcgcct tcatgccata
    10741 ttgtttcaac acaggatacg catagcggct caccgatttg aggccatcat caaaggtaat
    10801 caccaccgct cgcgcaggga gattgatctt attcttcacg taaccttcca gctgcaccat
    10861 actcagtgtc gtgtatcccc tgtcgcgcag ccaggccatc tggttattga aagcgcgtac
    10921 cgaggtggtc gtcgaagtat ggcgaaaacg ggtgttttct tcgtcgcgca gaatatggtg
    10981 ataggttagc accggcaggc cattatcggg ttgggcatcc agcgcgctga tataggccag
    11041 tcgatcgcca atacggatct gataccaggt ttgatttaac ctgtctttca gtttatgcaa
    11101 aatcgggtag cgcaaattgt ccgccagtac cccaaacggc gcactgcccg cactcggcgc
    11161 gttatagacc ggcgtatctt tccaggtaac taagttctga ttactcagcg gcttgttgag
    11221 gtcacccaaa ccgtcttcaa ctttttgtcg cccctgaacc ggctcgagat gacctttatc
    11281 gataaaccct ttaccaaagc caaaattaaa tgcgtaataa cttgcggcag tgggctccac
    11341 ggcaatgatt tgtccggccc gaatattccc caccgtcacc atcttgtcgc caatttgcgc
    11401 ccagaccgca gcattttcga tggtttgcat ataacgggca ggtaacgcgg cactgacact
    11461 tgcggtaaac agcatcagca ggagaataac agcttgtttg tacatgaaaa aataaccgag
    11521 ggtgaaacaa cgcttttatt gtagcaaaag cgccatcaga atcgcgggtt aatactcata
    11581 caaattatgt ttgaggacga aaggcggatt tttttgttgc tggtgccaaa gactactcac
    11641 ctccggcgca cccagttcga caatacgctc gcgaaactct gcgctggata acgtttcacg
    11701 gtaggccagc atctgctcaa ttaacggtaa cgtgacgcca gaaatcactt cgcagcggga
    11761 atgtttatgg cttaatagcg aagccacgcg gtacggcggc gctccggcta tatcggttaa
    11821 gaagatgaca ccctcaccag agtccgcttc atgcagagca tcgcacatca tgcggctgag
    11881 catattagag cttaacccac gccagaaatt cacggcccgg cactgaagaa gtgccccatg
    11941 ttttttctcc agcgcatcca gtatctcttg cgcccggtca tcgtgacagg taattaccca
    12001 acctaacatc gccttcctcc ttagccgaaa atcagtctac cctgaccatt gttgataaag
    12061 ggtgatagcc gtcaaaactc ctctccccca aatccagagg agaggaaata gccttagcta
    12121 cgcaaaccac gtccacgttg gatcagcgac caacagatta aataaaacgc cacaataaag
    12181 accaccagta cgccaaaggt agtgaccagc ggaacatcat tgataccgag gaagccgtag
    12241 cggaaaccac tgatcatata aacgattggg ttcaggtgcg acagcccttg ccagaacggc
    12301 ggcaacaaag tcagtgagta aaagaccccg cccaaatacg taagtggcgt taacacaaag
    12361 gttggcacca ggctgatgtc atcgaacgtt ttggcaaaca cgccgttcag caaacccgca
    12421 agggagaaca acaccgccgt gagcaccagc gttaaagcga caaataccca cgaatgcacc
    12481 tgaaacggca caaaaaacag tgaaattgcc gtcaccagaa tgccaacaaa cagaccacgc
    12541 gccacgccac cgccgacata tccggcaata atgacgtgag tcggaaccgg cgctaccagc
    12601 agctcttcaa tattacgctg gaacttggca ccaaaaaatg atgacgcgac gttagcgtag
    12661 gcgttggtga tcaccgacat catgatcagc cccggtacaa tgaactgcat gtagctgaag
    12721 ccatgcatat cgccaatacg cgaaccaatc aggttaccga agataataaa gtaaagggtc
    12781 atggtgatga ccggtggcac cagcgtctgc acccagatac gcataaagcg atggatctct
    12841 ttcgcccaga tgctttttag tgccacccag taaagatgca tcatgcgcga tctccttgtt
    12901 tttcattaac cagtgaaaca aacagctctt ccagacggtt agctttgtta cgcatactta
    12961 atacctgaat gccctgctca cttaactgcg taaatacgct gttgatcccc tgctcacgca
    13021 gaacttcaac ttccagcgtc gcggtatcga ccagtcgata ctgatagcca tcgagcttcg
    13081 gtaacgggct tttcggtgcg agatcgagaa taaaggtttc cgatttcagc ttcgccagca
    13141 gcgccttcat cgaggtattt tccaccagct caccgtgttg aataatgccg atattgcggc
    13201 acagcatttc tgcttcttcc aggtagtgtg tggtgagaat gatagtggtg cctttgtcgt
    13261 ttaaatcctt caaaaagccc cacattgagc ggcgaagttc gatatccacg cctgcggtcg
    13321 gttcgtcgag aatcagtagt ttaggttcat gcattaacgc acgggcaatc attaaacggc
    13381 gcttcatccc gccagataac atacgcgcac gttcgttgcg ttttccccat agatcgagtt
    13441 gtttaagata cttttcgctg cgaatgtacg cttctttgcg ctccacaccg tagtaccctg
    13501 cctgattcac cacaatttgc tgcacggttt caaacgggtt gaagttaaat tcctgtggca
    13561 ccagacccaa ctgacgttta gcgtttacga catctttctc gagatcgtaa ccaaatacgc
    13621 tgacccgccc ggaggtttta tttaccagag agctgataat accgatagtg gtcgattttc
    13681 cggccccgtt cggcccgaga agcgcataaa aatcacccgc ttcgacctgc aaatcgattc
    13741 cacgaagcgc ctgaacgccg cctggatagg tttttttaag ctgttgaagt tccagtgcaa
    13801 tggtcataaa tttttactta ccttacgttc ttacacttta tctgtggttt aaatcgtccc
    13861 ggagttgccc tatattagcc aaacgtaatt atttggttac aggtcgttaa cctccatgaa
    13921 agacatagat acactcatca gcaacaatgc actatggtca aaaatgctgg tggaagagga
    13981 tcccgggttt tttgagaaac tggcacaagc gcaaaaaccg cgctttctat ggattggatg
    14041 ttccgacagt cgcgttcctg cagaacgttt aaccggtctt gagccgggcg aactctttgt
    14101 tcaccgtaat gttgctaacc tggtcattca caccgacctg aactgccttt ccgtggttca
    14161 gtatgcagtg gatgtactcg aagttgaaca cattattatc tgtggccact acggttgcgg
    14221 cggcgtacaa gccgcagttg aaaacccgga actggggctt atcaacaact ggctgctgca
    14281 tatccgtgat atctggttca aacatagctc attgctcggc gaaatgccgc aagagcgccg
    14341 tctggatacc ttgtgtgaac tgaacgtcat ggaacaggtg tataacctgg gccactccac
    14401 cattatgcaa tcagcgtgga aacgcgggca gaaagtcacc atacacggct gggcctacgg
    14461 cattcacgac ggcttgctac gtgatctgga tgtcacagcg actaaccgcg aaacccttga
    14521 gcaacgttac cgtcacggga tctccaacct caagctgaaa cacgccaacc acaaataaaa
    14581 atgccatgcc ggatgcaaca catccggcaa cttcacactt actcgtccag cagaatcact
    14641 ttgccgatat acggcagatg acggtaacgc tgtgcgtaat caatgccgta acccaccaca
    14701 aactcatccg ggatcgagaa accgataaat tctaccggga cgttcacttc acgacgggac
    14761 ggtttatcca gcagcgtaca aatcgccaac gacttcggtt cgcgcaggct taagatctca
    14821 cgcactttcg acagtgtatt ccccgagtcg atgatatctt caacaatcag cacgtccttg
    14881 ccacggatat cttcatccag atctttgagg attttcacat cacgggtggt ggacatgccg
    14941 ctaccgtagc tggaggcggt cataaaatcg acttcatgag atacctgaac ttcacggcac
    15001 aggtccgcca taaacataaa tgagccacgc agcagaccaa ccagcaccat atcgctgcca
    15061 ctgtctttgt aacgctcagt aatctgacga cccagttcgg cgatacgcgc tttaatctcc
    15121 gcttcgggga tcattacttc tacagtatgt ttcatatctc taaccatatg atttaaaaac
    15181 aaatcacttc gcacgggcag gtttcagcac cgcaacaaag cgcaagacac gcagtatacc
    15241 agtaaaacta ttcattcgcg gaatctgtta ataaagctcg aattgtgatg acgatcacac
    15301 atgttaaaac ctataattaa ttgctactaa aatattaatg aattgaaatg gtgtctcttt
    15361 atggcaatta acaataccgg ctcgcggcga ttactcgtca cgctaacagc cctttttgca
    15421 gcgctttgcg ggctgtatct actcattggc ggaggctggc tggtcgcgat tggcggctcc
    15481 tggtactacc ctatcgctgg ccttgtgatg ctcggcgtcg cctggatgct gtggcgcagt
    15541 aaacgcgccg cgctttggct gtacgcagcc ctgctgctcg gcactatgat ttggggcgtc
    15601 tgggaagttg gtttcgactt ctgggcgctg actccgcgca gcgacattct ggtcttcttc
    15661 ggcatctggc tgatcctgcc gtttgtctgg cgtcgcctgg tcattcctgc cagcggcgca
    15721 gttgccgcac tggtggtggc actgctgatt agcggtggta tcctggcctg ggccggattt
    15781 aacgatccgc aggaaatcag cggcacctta agcgccgatg ccacacctgc cgaagccatc
    15841 tcccccgttg ccgatcagga ctggcctgcc tatggtcgca atcaggaagg tcaacgcttt
    15901 tcacctctga aacaaattaa cgccgataac gttcacaacc tgaaagaagc ctgggtgttc
    15961 cgtacaggcg atgtgaaaca gccgaacgat ccaggtgaaa tcaccaatga agtgacgcca
    16021 attaaagtgg gcgacaccct ttacctgtgt accgctcacc agcgcctgtt tgcgctcgat
    16081 gccgccagcg gcaaagagaa atggcattac gatcctgaac tgaaaaccaa cgagtccttc
    16141 cagcatgtaa cctgccgtgg cgtctcttat catgaagcca aagcagaaac tgcctcgcca
    16201 gaagtgatgg cggattgccc gcgtcgtatc attcttccgg tcaatgatgg tcgtctgatt
    16261 gcaattaacg ctgaaaacgg caagctgtgc gaaaccttcg ccaataaagg cgtgctcaat
    16321 ctgcaaagca atatgccgga caccaaaccg ggtctgtatg aaccgacttc gccgccgatt
    16381 atcaccgata aaaccatcgt gatggccggt tcagtcaccg ataacttctc aacccgtgaa
    16441 acatctggcg tgatccgtgg ttttgatgtc aacaccgggg agctgctgtg ggcttttgat
    16501 cctggtgcga aagatcctaa cgcaatcccg tctgacgaac acacctttac ctttaactcg
    16561 ccaaactcgt gggcaccagc ggcctatgac gcgaagctgg atctggtgta tctgccgatg
    16621 ggcgtgacca cgccggatat ctggggcggt aaccgcacac cggaacagga acgttatgcc
    16681 agctcgattc tggcgctgaa tgccacaacc gggaaactgg cgtggagcta ccagaccgtt
    16741 caccacgacc tgtgggatat ggatcttccg gcacagccga cgctggcgga tatcaccgtt
    16801 aatggtcaga aagtgccagt tatttatgct ccggcgaaaa ccggcaacat ttttgtgctc
    16861 gatcgtcgta atggcgaact ggtggttccg gcaccggaaa aaccggttcc ccaaggtgct
    16921 gccaaaggcg attacgtaac cccaactcaa ccgttttctg aactgagctt ccgtccgacg
    16981 aaaaatttga gcggtgcgga tatgtgggga gccaccatgt ttgaccagct ggtgtgccgc
    17041 gtgatgttcc accagatgcg ctatgaaggc attttcaccc cgccatctga acagggtacg
    17101 ctggtcttcc ctggtaacct ggggatgttc gaatggggcg ggatttccgt tgatccgaat
    17161 cgtgaagtgg caattgccaa cccaatggca ctgccgtttg tttcgaaact gatcccacgc
    17221 ggtcctggca acccgatgga gcagccgaaa gatgccaaag gcacgggtac ggaatccggc
    17281 attcagccgc agtacggtgt accgtatggc gtcacgctca atccgttcct ctcaccgttt
    17341 ggtctgccgt gtaaacagcc agcatggggt tatatctcgg cgctggatct gaaaaccaat
    17401 gaagtggtgt ggaagaaacg tattggtacg ccgcaggaca gtatgccatt cccgatgccg
    17461 gtacctgtgc cgttcaatat gggtatgccg atgctgggcg ggccaatctc cacggcgggt
    17521 aacgtgctgt ttatcgccgc tacggcagat aactacctgc gcgcttataa catgagcaac
    17581 ggtgaaaaac tgtggcaagg tcgtttacca gcgggtggtc aggcgacgcc aatgacctat
    17641 gaagtgaatg gtaagcagta tgtggtgatc tccgcaggcg gtcacggttc atttggtacg
    17701 aagatgggcg actatattgt ggcttatgcg ttgccggatg atgtgaagta aggcttgctc
    17761 agattgctga caacgtgcga tttgttcatg ccggatgcgg cgtgaacgcc ttatccggcc
    17821 tacaaaacat ttgcaaattc aatacattgc gcgtaggcct gataagcgta gcgcatcagg
    17881 cagttttgcg tttgtcatca gtttcatgcc cggagagatc cgggcatatt tccgaatacg
    17941 gtctttttat accgtaaacc ctaacatcat ccccgtatct tcatgctcca gcaaatggca
    18001 gtgcgccata taagcatgtt ctttcggtgc atcgtgatta aacttcacca gcacttcgct
    18061 gacattacct tctaccttaa cggtatcttt ccagcctgcg cgatgcgtcg ctggcggttt
    18121 gccattttct gacaagatac ggaactgcgt accgtggata tggaacggat gcagcatcat
    18181 gtcgcccacg ccagagataa cccaacgttc gtattgccct ttcgccgccg caaacatcgg
    18241 cttgttcata tcaaacgcct gaccgttgat tttattggca tggtggaaat cgaacttccc
    18301 gccgtggttc atatgattca tattgccatg ccccatatgg cccatcatct ggctgtgatc
    18361 catcccggcc atcgcctgat cgccatattt ctccatcagc atctgcatcc ccatcatatc
    18421 gagcatcggg tccatagaga gttgcagctt gcgtaccgtc agcccttcca gcgaaggtaa
    18481 cgcaggcagg ctacttaatg tgtctggcaa agcaccagag gcactaatgg caatcggctg
    18541 aatccgcatt accggatgcg gcttgtcaaa cggcgcaatc gccatcccca tctggctgac
    18601 cggcagcgtc accaggtcaa aggggttgtt gtcgttaacc tccaccagca cttcaaaacg
    18661 ctcgcccatc agcacgggaa gctcgttcac cttcaccggt tcaggtagca gaccgccgtc
    18721 gctggcaatc acataaagcg ggcgattgtc gctggtggcg aaattgagcg agcgggcatt
    18781 acagccattg agcaaacgca ggcgcagcca accacgcggg gcagcgtgtt gtgggtagat
    18841 tgcaccgttg gtcaacaacg tatcgccaaa ccagcccacg gcggcggtca tcacatccag
    18901 ttgataatca atctgcccgt cagcgctaaa tttcttatcc tgaacgatca ccggaacatc
    18961 atcgataccc cactgttttg gcagcattaa tttcaggatc tcgtcatctt caatcaccac
    19021 cagaccagcc agccccatcg ccacctgtcg cccggtcttg ccatgttgat gtggatggaa
    19081 ccagcaggta gcggcaggtt gatcaacgtt caacgtcacc gagcgcttgc cacctggcgg
    19141 aataattccc tgcgggccgc cgtccacttc acccggtact tccagcccgt gccagtgcaa
    19201 cgtcgtctct tccgtcagtt ggttgtagat atcaaccgtt accgctttgc cgcgctgtaa
    19261 tttcaccgcc ggccccagca gattgccgtt atagccccag gtagttgcgg ttttcccgcc
    19321 aaaggtggac tgacctgcgc caatagttaa ctgaatgcga ttacgggcat cggtcgtgag
    19381 caaatcaggg atcggtaacg ttgggcgttc cgccgcaaat actgcgcggc tccacagcgg
    19441 caaagccgaa gccacgccca gcgcgacgga atattttaag aaatcacgac gttgcatagt
    19501 tatttcctta ttcttaagca ggcgaaacaa aatcaaacgt tgagcatagt ccttcccctt
    19561 acgggaaggt caagccgcag acacaattta atcgacaata atggtcgtcg cctcgctcgc
    19621 agtgtgctaa cgtttatctt ctttaagccc ctggtagaag caatgaagac gtttttcaga
    19681 acagtgttat tcggcagcct gatggccgtc tgcgcaaaca gttacgcgct cagcgagtct
    19741 gaagccgaag atatggccga tttaacggca gtttttgttt ttctgaagaa cgattgtggt
    19801 taccagaact tacctaacgg gcaaattcgt cgcgcactgg tcttttttgc ccagcaaaac
    19861 cagtgggatc tcagtaatta cgacaccttc gacatgaaag ccctcggtga agacagctac
    19921 cgcgatctca gcggcattgg cattcccgtc gctaaaaagt gcaaagccct ggcccgcgat
    19981 tccttaagcc tgcttgccta cgtcaaataa tccctgatac ctttttgtag aaatattgac
    20041 cgtgcatccg cggtcaatgt tagctattat gttgcgccct ttttttacgg gtgttaacaa
    20101 aggaggtatc aacccatggc cgaaaaaaaa cagtggcatg aaacgctaca cgaccagttt
    20161 gggcagtact ttgcggtaga taacgttctg tatcatgaaa agaccgatca ccaggatctg
    20221 atcatttttg agaacgccgc atttggtcgc gtaatggcgc tggatggcgt agtacaaacc
    20281 accgagcgcg acgagtttat ctatcatgag atgatgaccc atgttccgct actggcccac
    20341 ggtcacgcga aacatgtgct gattatcggc ggcggcgacg gtgccatgct gcgtgaagta
    20401 acccgacata aaaacgttga gtcaatcacg atggtggaaa tcgatgcggg tgtcgtgtcg
    20461 ttctgccgtc agtatcttcc caaccataac gccggtagtt acgacgatcc acgctttaaa
    20521 ctggtgatcg acgatggtgt caatttcgtt aatcaaacca gccagacctt tgatgtcatt
    20581 atctccgact gcaccgatcc tatcggtccc ggcgaaagtc tgtttacttc tgcattttat
    20641 gaaggctgca aacgttgcct gaatcctggc ggtatcttcg tcgcacaaaa cggcgtctgc
    20701 tttttacagc aggaagaggc catcgacagt catcgcaaac tcagccatta cttcagcgat
    20761 gttggctttt atcaggcggc gatcccgacc tattacggcg gcatcatgac ctttgcatgg
    20821 gcgacagata acgacgcctt acgccatctc tcaaccgaaa ttattcaggc gcgttttctc
    20881 gcttctggcc tgaaatgccg ttattacaat ccggcagtcc atacggcagc ttttgcctta
    20941 ccccagtatc tgcaagatgc actggcttca cagccgtcct aaggagaaga taagaaattg
    21001 aaaaaactga aactgcatgg ctttaataat ctgaccaaaa gtctgagttt ttgtatttac
    21061 gatatctgct acgccaaaac taccgaagag cgcgacggtt atattgctta tatcgatgaa
    21121 ctctataatg ccaaccgtct gaccgaaatc ctgtcagaaa cctgttccat tatcggggcc
    21181 aatattctta acatcgcccg ccaggattac gaaccacagg gtgccagcgt cactattctg
    21241 gtgagtgaag aaccggttga cccgaaactc atcgacaaaa cagaacaccc cggcccactg
    21301 ccagaaacgg tcgttgccca tctcgataaa agtcatattt gcgtacatac ctacccggaa
    21361 agtcatcctg aaggcggttt atgtaccttc cgcgccgata ttgaagtctc tacctgcggc
    21421 gtgatttctc cgctgaaggc gctgaattac ctgatccacc agcttgagtc cgatattgtg
    21481 actattgatt atcgcgtgcg cggttttacc cgcgacatta acggtatgaa gcactttatc
    21541 gaccatgaga ttaattcgat tcagaacttt atgtccgaag atatgaaggc gctgtatgac
    21601 atggtggatg taaacgtcta tcaggaaaat atcttccata ccaagatgtt gcttaaagag
    21661 ttcgacctta agcactacat gttccacacc aaaccggaag atttaaccga cagcgagcgc
    21721 caggaaatta ccgctgcgct gtggaaagaa atgcgcgaga tttattacgg gcgcaatatg
    21781 cccgctgttt aacgtctcag gcggagcacg gaggctccgc ctgatttact tacttctgct
    21841 gcacgaaatt acggtaagcc gccaccacct gaagaaaatc ctcaacgccg cacagcgaca
    21901 agctctcttc atcgtagtag ttcatcccct cttccatttc atcgccagcg aattccagtt
    21961 gattggcgcg aaccatcacc tcttcaccgt ccatccacag agtgtattca tgccctgccc
    22021 actgccagga tcgttcgcta cctttcaatg cgtgcgccgc ttgttccact tcatcaagca
    22081 aggccaggtt ttctttcacc tcttcattaa accagtgccc gaccacttca tgccccatgg
    22141 acatacgcac ctttaccact ccggtaatat cgcgcagaaa ttcgtaatcc atagtgtgtt
    22201 cctctgctcc cggcaatgcg ccgaaatcgc attgcgccat tgatgtaatt atcgcagcct
    22261 taccggagaa aaacagcgga acggcgatgg tttagaaata aaaaatgccc gggaatctca
    22321 ttcaccgggc attgtgtcgt ttatgcgcag cgcgtgcgct gactttttaa accgcagtct
    22381 ggaaaatcac cccatcggct ttttcggtgt actgagaaag ctggttgaag ttcagataac
    22441 ggtaagtatc aacggcagtt ttatctactt gcgccacgta ggtctggtac tcttccggcg
    22501 tcggcagttt gccaatcagc gccgcaacag cagccagttc cgcagaagcc aggaagacat
    22561 tcgcgcccgt ccccaaacgg ttcgggaagt tacgggtaga ggtggaaacc accgtcgcac
    22621 cgtctgctac acgcgcctgg ttacccatac acagggaaca gccagggatc tcgatacgag
    22681 caccactctt accgaagacg ctgtagtagc cttcttcggt cagctgtgca gcgtccatac
    22741 gcgttggcgg tgccacccac aggcgggtcg gcaactgacc tttgtgcgca tccagcagtt
    22801 tacccgcagc acggaagtga ccgatgttag tcatgcaaga accgataaac acttcgtcga
    22861 tcttctcgcc ctgtaccgca gacagcggac gagcgtcgtc cgggtcgttc ggtgcacaga
    22921 ggattggctc tttaatatcc gccagatcga tgtcgatcac tgccgcgtat tccgcatctg
    22981 catcggcttc cagcagctca ggattcgcca gccatttttc catgccctga atacgacgtt
    23041 ccagggtacg acgatcgccg taaccttccg cgatcatcca cttcagcagg acgatgttag
    23101 agttcaggta ttcgatgatc ggttctttgt tcagcttgat ggtacaaccg gcagcagaac
    23161 gctcggcaga cgcatcggtc agctcaaatg cctgctcaac tttcagatcc ggcagacctt
    23221 caatttccag aatgcgacca gagaagatgt ttttcttgcc tttcttctca acggtcagca
    23281 gaccttgttt gattgcatac agcggaatgg cgtgaaccag gtcgcgcagg gtgatgcccg
    23341 gctgcatttt gcctttgaag cgcaccagaa cggattccgg catatccagc ggcattacgc
    23401 ctgttgcggc tgcaaatgcc accagaccag aacccgccgg gaaagagata ccgatcggga
    23461 aacgggtatg ggagtcacca ccggtaccga cagtatccgg cagcagcata cggttcagcc
    23521 aggagtggat tacgccgtca cccggacgca gcgacacacc gccacggttc ataatgaagt
    23581 ccggcagcgt gtggtgcgtg ttaacgtcaa ctggtttcgg atacgccgcg gtgtggcaga
    23641 aagactgcat caccaggtca gccgagaagc ccaggcacgc caggtctttc agttcatcac
    23701 gagtcatcgg gccggtggtg tcctgggaac ctacagaagt cattttcggt tcacagtacg
    23761 cgcccggacg aatgcctttc acgccacagg cacggcctac cattttctgc gccagcgaga
    23821 agccgcgatc gctctcagcg acatctttcg cctgacggaa cacatcgctg tgcggcaggc
    23881 caagtgcttc acgcgctttg gtggtcaggc cacgcccaat aatcagcgga atacggccgc
    23941 cagcacgcac ttcatcaatc agcacgtcgg ttttcagttc gaaggtcgcc agcagttcgc
    24001 cggtttcgtg gttacgcact tcacctttgt acgggtaaac gtcaattacg tcgcccatgt
    24061 tcaggttaga gacgtcgact tcgattggca gcgcacccgc gtcttccatc gtgttaaaga
    24121 agatgggtgc aattttaccg ccgaggcaca aaccaccgcc gcgtttgttc ggcacatgtg
    24181 gaatatcatc gcccataaac cacagaacgg agttagtggc ggatttacgc gatgaccccg
    24241 tacccacaac gtcaccgacg tacgccagtg ggaaaccttt ctgttgcaga gcttcgattt
    24301 gcttaatcgg accaacaacg cctggctgat ctggctcaat accttcacgg gcgtttttca
    24361 gcatcgccag cgcgtgcagt gggatatccg ggcgtgacca cgcatccggt gccggagaga
    24421 ggtcatcggt gttggtttcg cctgtgactt tgaagacggt aacggtcagt ttttcagcca
    24481 gcgccgggcg attcaggaac cattcggcat ctgcccagga ctgcattacc tgcttcgcat
    24541 attcgttgcc tgctttcgct ttctcttcta cgtcatagaa gttatcgaac atcagcagcg
    24601 tgtgagaaag tgctttggca gcgatcggtg ccagtttggc atcatccagc gcgtcgatca
    24661 gcggatgaat gttgtaacca ccctgcatgg tgcccagcag ttcgatggct ttttccggag
    24721 tcagcagagg ggatttggct tcgcctttag cgatagcagc caggaagcct gcttttacat
    24781 aggcggcttc atcgacgcct gggggaacac ggttggttaa cagatctaac aggaattctt
    24841 cttcgcccgc gggcgggttt ttcagcagct ctacaagcgc ggccatttgg tttgcatcca
    24901 ggggtttggg cgcaatcccc tcagcggcac gctcagctac gtgcttacgg tattcttcta
    24961 gcacgacggt tctcctcgct ctcattgtca tagtgcggca ggcgattctc ttcacgctcc
    25021 tgtgagacag cagtttgtag ggtaaatgcc cagtaccgca acgggcagaa tagcaggatt
    25081 ttgacgatgt gttaatctgt ttacaaaaaa gcaacataaa aaagtggctg aatcgttaag
    25141 gatggtctag agattgtttc cctccgtaaa aacttcgtta attcgcatgg tgataatcac
    25201 ctttctcaac gaatcccaag attagtcaaa atttaaacta ccgcctcttt atactcggat
    25261 tcacagcacc tgcgggtggc agttcgccga ccattgcgat ttccttgaga tccgaattat
    25321 gaaaatgact ttgccgttta aaccccatgt gctggcgcta atttgcagtg ccgggctttg
    25381 tgccgcctct accgggctat atataaaaag ccgcacagtg gaagcgcctg tggaaccgca
    25441 atcgacacaa ctggctgcgc ctgacatctc cgcagttacg cttcctgcaa cggtttccgt
    25501 gcctcctgta acacctgccg tcgtcaaatc cgcattcagc actgcgcaaa tagatcaatg
    25561 ggttgcacct gtcgcgctat accccgactc tctgctttca caagtgttaa tggcatcaac
    25621 ctatccggca aacgttgctc aagcagtgca atggtcgcac gataatccac ttaaacaagg
    25681 cgatgctgct attcaggcgg tatctgacca gccctgggac gccagcgtca aatcactggt
    25741 ggcctttcca cagttgatgg cattgatggg cgaaaacccg caatgggtgc aaaacctggg
    25801 cgatgctttt ctggcgcagc cgcaggaggt gatggactcg gtacagcgat tgcggcaact
    25861 ggcgcaacaa accggctcgc tgaagtcatc aactgaacag aaagttatta ccacaacgaa
    25921 gaaagctgta ccggtaaaac agacagtcac ggctcccgtc ataccatcca ataccgtttc
    25981 aactgccaac cctgtcatta cagagcctgc aacaaccgtc atttccattg aacccaccaa
    26041 tcctgatgtg gtctatattc ccaactacaa gccaaccgtg gtttacggga actgggccaa
    26101 tactgcgtat ccgccggttt atctgccgcc gccagccgga gaaccgtttg ttgacagctt
    26161 tgtgcgcgga ttcggctata gcatgggcgt tgctaccacg tacgcgctat tcagcagcat
    26221 cgactgggat gacgacgatc atgaccatca tcatcatgac gatgatgatt atcaccatca
    26281 cgatggcggt catcgtgatg gtaatggctg gcaacacaat ggcgacaaca tcaatatcga
    26341 cgtcaacaat ttcaaccgta tcaccggtga gcatcttact gataagaata tggcatggcg
    26401 gcacaatcca aactaccgta atggtgtgcc ctatcatgat caggatatgg caaagcggtt
    26461 tcatcaaacc gatgtcaacg gcggaatgag cgccacgcag ctacctgctc caacacgcga
    26521 cagccagcgt caggcggcag caagtcagtt tcagcaacga acacacgcca cacccgtcat
    26581 tacgcgagat acccaacgtc aggcagcggc acagcggttt aatgaagctg aaaactatgg
    26641 gagctatgac gacttccgcg acttcagccg tcgccaacca ctgacccagc aacaaaagga
    26701 cgccgctcgt cagcgttatc agtcggcctc tcctgagcag cgccaggcag tccgcgagaa
    26761 aatgcagact aacccgcaga tccagcagcg aagagaggca gcgcgtgagc gcattcagtc
    26821 tgcaactccc gaacagcgcc aggtgtttaa ggaaaaagta cagcagcgcc cactgaatca
    26881 acagcaacgt gataacgccc gccagcgtgt tcaatcagca tcacctgagc aacgtcaggt
    26941 ttttcgggag aaagttcagg agagccgccc gcaacgtcta aacgacagta accgtactgc
    27001 caggctgaat aacgagcaac ggtcagcagt acgcgaacgg ctgtctgaac gtggagcaag
    27061 acggctggaa aggtaaatta caggcgtaaa aaaagcggcg tggttagccg cttttttaat
    27121 tgccagatgt cccggcaaac gaaaaattac ttcttcttcg ctttcgggtt cggcaggtcg
    27181 gtaatgctac cttcgaacac ttctgccgcc aggcccacag actcgtgcag cgtcgggtgc
    27241 gcatggatgg tcagtgcgat gtcttcagcg tcacaaccca tttcgattgc caggccgatt
    27301 tcacccagca gctcgccgcc gttagtaccg acaatcgcac caccgatcac acggtgagat
    27361 tctttgtcga aaatcagctt ggtcataccg tctgcgcagt cggaagcgat agcacgacca
    27421 gaagcagccc acgggaaggt ggcggtttca tagctgatgc ctttctcttt cgcttctttc
    27481 tcggtcagac caacccatgc aacttctggt tcggtatagg cgatggacgg gataactttc
    27541 ggatcgaagt aatgtttttt accggcgata acttcagcgg caacgtgacc ttcgtgaaca
    27601 cctttgtgtg ccagcatcgg ctgaccgacg atatcgccga tagcaaagat gtgcggtacg
    27661 ttggtacgca gctgtttgtc aacgcggatg aaaccacggt cgtcaacttc cacgccagct
    27721 ttgcctgcgt cgaggttttt accgttcggc acacgaccaa tcgctaccag cacggcgtcg
    27781 taacgctgcg gttcagcggg tgcttttttg ccttccatcg tcacataaat gccgtcttct
    27841 ttcgcttcaa cggcggtaac tttggtttcc agcatcaggt tgaatttctt gctgatacgc
    27901 ttggtgaaga ctttaacgat gtctttgtca gctgccggga taacctggtc gaacatttca
    27961 accacatcaa tctgtgaacc cagcgcgtgg tatacggtcc ccatttccag accgatgata
    28021 ccgccaccca ttaccagcag gcgttctggt acttctttca gttccagcgc gtcagtggag
    28081 tcccagatac gcggatcttc atgcggaata aacggcagtt ggatcgggcg agaacccgct
    28141 gcaatgatcg cgttgtcgaa gttgatcacg gttttgccgt tctcaccttc aacttccagg
    28201 gtgttagccc cggtgaattt acccagaccg ttgaccactt tgactttgcg gccttttgcc
    28261 ataccagcca gaccaccggt cagctgattg atcactttct ctttccaggt acgaatcttg
    28321 tcgatatcgg ttttcggttc gccgaagacg ataccgtgtt cagccagcgc tttggcttct
    28381 tcgataactt ttgctacgtg cagcagtgct ttagaaggga tacagccgac gttcaggcaa
    28441 acaccgccaa gggtggcgta acgttctacg attacggttt ccagacctaa atcagcgcaa
    28501 cggaaggcag cggagtaacc tgcggggcct gccccaagta ccacgacctg agttttgatt
    28561 tcagtactca tcatgacctc tatatattta tctccggcgg tcatacccgt cgtctttcag
    28621 gccgcagcgg cgttagcttc acgccctcac cccagtcact tacttatgta agctcctggg
    28681 gattccggcg cttgctacct tgctgcaccc agaaatccat agggtatgtg aagtaatacc
    28741 ctaaccacca ccgggtcgtt ctatccgtcg gtattttaca aaattgttaa caatttttaa
    28801 acaacaaacg gcaaccgatt tgtctattcg ctaataacct caatacattc atgagattac
    28861 cagaaaaaag ccggccgttg ggccggctct tttacttaca tcaccagacg gcgaatgtca
    28921 gacagcgtgt tgttaatgat ggtaatgaaa cgggcaccat cagcaccgtc gatcacgcgg
    28981 tggtcgaagg agagagaaat cggcagcatc agacgcggca cgaactcttt accattccac
    29041 accggctcca tcgcggactt ggaaacgccg aggatagcca cttccggcgc gttcacaatc
    29101 ggcgcgaagt gggtagtccc caggccgccg atgctggaga tggtgaagca accgccctgc
    29161 atttcgcccg cagtcagctt accgtcacgc gctttcttag aaatagtcat cagctcgcga
    29221 gacagctcga tgatgccttt cttgttgacg tctttgaata ccggaacaac cagaccgttc
    29281 ggggtatcca ccgccacacc gatgttgatg tatttcttca gggtcagacg ctgaccgtct
    29341 tccgacaacg aactgttgaa gcgaggcatc tgctcaagag ctgcagcaac ggctttcatg
    29401 atgaagacaa ctggggttat cttcacatcc agcttacgtt tcgccgcttc ttcgttctgc
    29461 tgtttacgga acgcttccag ctcggtgata tcggttttgt cgaagtgggt aacatgcggg
    29521 atcatcaccc agttacggct caggttcgca ccagagattt tctggatgcg gcccagttcc
    29581 acttcttcga tttcaccaaa cttgctgaag tctaccttcg gccacggcaa catgcccggg
    29641 ataccgccgc cagtcgccgc cggagccgct tctgcacgtt tgatagcttc tttcacgtaa
    29701 gcctgaacgt cttcgcgcag gatacgacct ttacggccag tgcccttcac tttcgccagg
    29761 ttaacgccaa attcgcgtgc cagacggcgg atcagcggag tcgcgtgaac gtaagcgtcg
    29821 ttttcagcaa attcagattt gccttcggct ttcgcagccg gagctgctgc tggagcttca
    29881 gcttttgctg ccggagccgg cgctgccgct tcctgtttca caggagctgc cgcaggcgct
    29941 gcgccttcaa cttcgaagat cataatcagc gagccagttt tcactttatc gccaacgttg
    30001 actttcagtt ccttcacgac gcctgcaaac ggagccggaa cttccataga cgctttatcg
    30061 ccttctacgg tgatcagtga ctgttcagcg gcaactttgt cgcccacttt caccatcact
    30121 tcggtcactt caacttcgtc accgccgata tccggaacgt taacttcttt cacgccagcc
    30181 gctggtgcag gggccgctgc cggagccgct tcctgtttag ccgccggagc tgccgcgcct
    30241 gcttcacccg cgacttcgaa gaccataatc agcgagccgg tagacacttt gtcacccacg
    30301 ttcactttga tctctttcac ggtgccagca aacggagccg gaacttccat agaagccttg
    30361 tcgccttcta cggtgatcag cgactgttca gcttcaactt tatcgccaac tttcaccagg
    30421 atttcggtca cttcaacttc gtcgctgccg atatccggaa cattaacgtc ttttgccgcg
    30481 gcagccgctg gtgctgctgc cggagcggct tctttcttct cttctgcctg agcaggtgca
    30541 gcgtctgctg caccgtcggc ggaatcgaaa atcataatca gtgcgccggt ctgggtttta
    30601 tcgccaacag agactttgat ctctttaacg atacccgcct gcggagacgg aacttccata
    30661 gaggctttgt cgccttctac ggtgatcagc gactgttcgg cttcaacttt gtcgcccact
    30721 ttgaccagga tctcggtgat ttcaacttca tcagccccga tgtccggtac tttgatttcg
    30781 atagccatta ttcttttacc tcttacgcca gacgcgggtt aactttatct gcatcgatgt
    30841 tgaatttggc gattgcgtca gcaaccactt tcttatcgat ttcgccacgt ttagccagtt
    30901 cgcccagcgc cgcaaccacg acataagaag catcaacttc aaagtggtga cgcaggttct
    30961 cacggctgtc ggaacgaccg aagccatcag tacccagtac gcggtagtcg tcagccggta
    31021 cgtaagtacg gacctgctca gcgaacagtt tcatatagtc ggtagatgcc actgccggag
    31081 cgtcgttcat cacctgagcg atatacggaa cgcgcggagt ttccagcggg tgcagcatgt
    31141 tccagcgttc acaatcctga ccatcacgcg ccagttcggt gaaggaagtc acgctataaa
    31201 cgtcagaacc tacgccgtaa tctttcgcca ggatctctgc tgcttcacgg acgtgacgca
    31261 ggatagaacc ggagcccagc agctgaactt tacctttgct accttcaatg gtttcgagtt
    31321 tgtagatacc tttacggata ccttcctcag caccttccgg cattgctggc atgtggtagt
    31381 tttcgttcag cgtagtgatg tagtagtaaa cgttctcttg tttctcaccg tacatacgct
    31441 ccagaccgtc atgcataatg acagcaactt cgtaagcgta agccgggtcg taagagatac
    31501 agttcgggat agtcagcgac tgaatgtggc tgtgaccatc ttcgtgctgc agaccttcgc
    31561 cgttcagggt ggtacgaccg gaagtaccgc cgatcaggaa gccacgcgct tgctggtcac
    31621 cagccgccca gcacagatcg ccgatacgct ggaagccgaa catcgagtaa tagatgtaga
    31681 acggaatcat cggcagattg ttggtgctgt aagaggtcgc cgctgccagc caggaacacc
    31741 cagcgcccag ctcgttgatc ccttcctgca gaatctgacc tttctcgtct tctttatagt
    31801 aagcaacctg ctcgcggtcc tgcggggtgt actgctgacc gttcgggctg taaataccaa
    31861 tctgacggaa cagaccttcc ataccgaaag tacgcgcttc gtcggcgatg atcggtacca
    31921 gacgatcttt gatcgacttg ttcttcagca ttacgttcag ggcacgaacg aaagcgatag
    31981 tggtagagat ctctttgctc tgctcttcca gcagcgcgcc gaagtcttgc aggctcggca
    32041 gctcaagctt ctcggtgaag ttcggctgac ggcttggcag ataaccgtgc agtttctgac
    32101 gctgagcgtg cagataggta tgctcttcag aaccttccgg gaaggtgatg tacggcaatt
    32161 tttcgatatc ggcatcagac accggcacat tgaaacggtc gcggatgtga cgcacgccgt
    32221 ccatgttcat tttcttaacc tggtgcgcga tgtttttacc ttcagccgcg tcgcccatgc
    32281 cgtaaccttt aatggtatga gcaaggatta ccgtcgcttt gcctttggtt tcctgcgctt
    32341 tcttaaatgc agcgtagatt ttcttcggat cgtgaccacc acggttcagt gcccagatct
    32401 gttcgtcagt ccagtctgca accagtgctg cggtttcagg atatttaccg aagaagtgtt
    32461 cacgaacgta cgcaccatct ttcgatttga aggtctggta gtcgccgtca acggtttcgt
    32521 tcatcagctg gatcagttta ccgctggtat ctttacgcag cagttcatcc caacggctac
    32581 cccacatcac tttgatcacg ttccagccag caccttcgaa gatgccttcc agttcgttga
    32641 tgatcttgcc gttaccggtg accgggccgt caagacgctg caggttacag ttgataacga
    32701 aaaccaggtt atccagtttt tcacgggtag cgatggtgat cgcacctttg gattccggtt
    32761 cgtccatttc accgtcgccg aggaacgcgt aaacggtttg tttagaggta tctttcaggc
    32821 cacggtgttc cagatatttc aggaatttag cctggtaaat agcaccaatc ggccccagac
    32881 ccatagatac ggtcgggaac tgccagaatt ccggcatcag tttcgggtgc ggataggaag
    32941 agaggccatt gccgtgaact tcctgacgga agttatccag ctgctcctga gtcagacgac
    33001 cttccaggaa agcacgagcg taaacacccg gggagatgtg gccctggaag taaaccaggt
    33061 cgccgccatc ctgctcgttg cgtgcacgga agaagtggtt aaagcacaca tcataaatgg
    33121 ttgcggaaga ctggaaggac gccatgtggc cgcccagttc gaggtctttt ttcgacgcac
    33181 gcagcaccgt catgatggcg ttccagcgga tagctgaacg aatacggcgt tccagttcca
    33241 gattacccgg atactccggt tgttcttcaa cggggatggt gttgatgtag ttgctgatac
    33301 cggtgcctgc ggctacgttg acaccgcctt tgcgggcttc agcaagcagt tggtcgatca
    33361 gatactgagc acgctcaaca ccttcttcac ggatgaccga ttcgatcgcc tggagccagt
    33421 cgcgagtttc gatcggatcc acgtcatttg ggaaacgttc tgacatgggt tattccttat
    33481 ctatctaata acgttgagtt ttctggaacc tgtcccattg aactctcgcc ggaaagctca
    33541 ataagacagg ttctacgttt agttgccgcg cttttatatg cgcttgattt acaacatctt
    33601 ctggataatt tttaccagaa aaatcactaa ttctttcgtt gctccagacg acgtagagaa
    33661 cgctcacggc ggctctcttc acggcttctg tcgagcaaaa tttcttcgat aaaggccaga
    33721 tggcgatgcg atgcttcgcg cgcttcttcc ggcttaccgg ccataatcgc ttcaaatatg
    33781 cgggtgcggt gactactcac cagcggcagc atctcgcgac gcgaatagag caattcgaag
    33841 ttctggcgga cattctgggc caacatcggc tccatacacc ttagcagatg aagcagaacc
    33901 acattgtggg ccgcttcggt gacggcaatc tgatactgga gtacggcatt tgattccgcg
    33961 tccagatcgc cagactgctg cgccagctct atggcgtggt ggagttcacg gatgcgttcc
    34021 ttgtcttcat cggtactacg cagcgcggcg taataagcgg cgataccttc cagggcgtgt
    34081 cgtgtttcga gcaagtcata ctgtgattca ggatggtcgg agagcagctc caccagcgga
    34141 tcgctgaagc tttgccatag gctgctctgg acaaaagtgc cgccaccctg gcgacgaagc
    34201 aacaagccct tcgcttcgag acgttgaatc gcctcacgca atgagggacg ggagacgtca
    34261 aactgttttg ccagttcgcg ttccggtggg agtttttcgc ccgggcggag agtgccttcg
    34321 aggatcaaaa actccagttg ctgctcaatc acatcggaga gttttggttg gcggattttg
    34381 ctgtaggcca tgagttcctg tcttaagcca cttgccgaag tcaattggtc ttaccaattt
    34441 catgtctgtg acgctaaagt aacaaagtat tcaccttatg tccatacagg ttttgattga
    34501 aatcatgaaa ctgtgcacat tttaacaact tgacatatat aacgtttcaa agttgtaact
    34561 atgcacaaat gtagacttta cg
//
LOCUS       DAPHCV010000049        34433 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.049, whole genome shotgun sequence.
ACCESSION   DAPHCV010000049 DAPHCV010000000
VERSION     DAPHCV010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.049"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(41..922)
                     /gene="htpX"
                     /locus_tag="RH033_002303"
     CDS             complement(41..922)
                     /gene="htpX"
                     /locus_tag="RH033_002303"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HDV2991479.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(1114..3162)
                     /gene="prc"
                     /locus_tag="RH033_002304"
     CDS             complement(1114..3162)
                     /gene="prc"
                     /locus_tag="RH033_002304"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HDV2991480.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQTFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPSGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(3182..3880)
                     /gene="proQ"
                     /locus_tag="RH033_002305"
     CDS             complement(3182..3880)
                     /gene="proQ"
                     /locus_tag="RH033_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HDV2991481.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(3977..4474)
                     /gene="msrC"
                     /locus_tag="RH033_002306"
     CDS             complement(3977..4474)
                     /gene="msrC"
                     /locus_tag="RH033_002306"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HDV2991482.1"
                     /translation="MNKIEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHVFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            4604..5887
                     /gene="yebS"
                     /locus_tag="RH033_002307"
     CDS             4604..5887
                     /gene="yebS"
                     /locus_tag="RH033_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HDV2991483.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPTTRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            5856..8489
                     /gene="yebT"
                     /locus_tag="RH033_002308"
     CDS             5856..8489
                     /gene="yebT"
                     /locus_tag="RH033_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HDV2991484.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLQEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            8569..10008
                     /gene="rsmF"
                     /locus_tag="RH033_002309"
     CDS             8569..10008
                     /gene="rsmF"
                     /locus_tag="RH033_002309"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HDV2991485.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCRWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKEVWLFPVDIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            10126..10362
                     /locus_tag="RH033_002310"
     CDS             10126..10362
                     /locus_tag="RH033_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HDV2991486.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            10467..10658
                     /locus_tag="RH033_002311"
     CDS             10467..10658
                     /locus_tag="RH033_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HDV2991487.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDIYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(10659..11315)
                     /gene="pphA"
                     /locus_tag="RH033_002312"
     CDS             complement(10659..11315)
                     /gene="pphA"
                     /locus_tag="RH033_002312"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HDV2991488.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASRQMSLWLMNGGD
                     WFIALADNHQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     ILWSRSRLGEHQKGQGIAGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(11710..12051)
                     /locus_tag="RH033_002313"
     CDS             complement(11710..12051)
                     /locus_tag="RH033_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HDV2991489.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(12064..12936)
                     /gene="copD"
                     /locus_tag="RH033_002314"
     CDS             complement(12064..12936)
                     /gene="copD"
                     /locus_tag="RH033_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HDV2991490.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWSDVFSPNIWLAVLQTQFGGIWLWQIVLAL
                     VTLIVALMQPQNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLMLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(12940..13314)
                     /gene="yobA"
                     /locus_tag="RH033_002315"
     CDS             complement(12940..13314)
                     /gene="yobA"
                     /locus_tag="RH033_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HDV2991491.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            13453..13683
                     /gene="holE"
                     /locus_tag="RH033_002316"
     CDS             13453..13683
                     /gene="holE"
                     /locus_tag="RH033_002316"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HDV2991492.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRNWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            13785..14441
                     /locus_tag="RH033_002317"
     CDS             13785..14441
                     /locus_tag="RH033_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDV2991493.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVSAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASTRR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            14465..15127
                     /gene="exoX"
                     /locus_tag="RH033_002318"
     CDS             14465..15127
                     /gene="exoX"
                     /locus_tag="RH033_002318"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HDV2991494.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(15124..17184)
                     /gene="ptrB"
                     /locus_tag="RH033_002319"
     CDS             complement(15124..17184)
                     /gene="ptrB"
                     /locus_tag="RH033_002319"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HDV2991495.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRDNIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPED"
     gene            complement(17393..18052)
                     /locus_tag="RH033_002320"
     CDS             complement(17393..18052)
                     /locus_tag="RH033_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HDV2991496.1"
                     /translation="MANWLNQLQSLLGQSRSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEKGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(18379..18735)
                     /locus_tag="RH033_002321"
     CDS             complement(18379..18735)
                     /locus_tag="RH033_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HDV2991497.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(18802..19092)
                     /gene="yebG"
                     /locus_tag="RH033_002322"
     CDS             complement(18802..19092)
                     /gene="yebG"
                     /locus_tag="RH033_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HDV2991498.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            19226..20404
                     /gene="purT"
                     /locus_tag="RH033_002323"
     CDS             19226..20404
                     /gene="purT"
                     /locus_tag="RH033_002323"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HDV2991499.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADNENLFREAVAEIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAHAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSQLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGAGIQIRLFGKPEIDGSRRLGVALATAESVDEAIERAKHAAGQVKVQG
                     "
     gene            complement(20460..21101)
                     /gene="kdgA"
                     /locus_tag="RH033_002324"
                     /gene_synonym="eda"
     CDS             complement(20460..21101)
                     /gene="kdgA"
                     /locus_tag="RH033_002324"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDV2991500.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(21138..22949)
                     /gene="edd"
                     /locus_tag="RH033_002325"
     CDS             complement(21138..22949)
                     /gene="edd"
                     /locus_tag="RH033_002325"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HDV2991501.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDNNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(23184..24659)
                     /gene="zwf"
                     /locus_tag="RH033_002326"
     CDS             complement(23184..24659)
                     /gene="zwf"
                     /locus_tag="RH033_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDV2991502.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            24997..25866
                     /gene="hexR"
                     /locus_tag="RH033_002327"
                     /gene_synonym="yebK"
     CDS             24997..25866
                     /gene="hexR"
                     /locus_tag="RH033_002327"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HDV2991503.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKPLLNLSDDR"
     gene            25994..27436
                     /gene="pyk"
                     /locus_tag="RH033_002328"
     CDS             25994..27436
                     /gene="pyk"
                     /locus_tag="RH033_002328"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDV2991504.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(27568..28539)
                     /gene="lpxM"
                     /locus_tag="RH033_002329"
                     /gene_synonym="msbB"
     CDS             complement(27568..28539)
                     /gene="lpxM"
                     /locus_tag="RH033_002329"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HDV2991505.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(28658..29980)
                     /gene="mepM"
                     /locus_tag="RH033_002330"
     CDS             complement(28658..29980)
                     /gene="mepM"
                     /locus_tag="RH033_002330"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HDV2991506.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(29996..30928)
                     /gene="znuA"
                     /locus_tag="RH033_002331"
     CDS             complement(29996..30928)
                     /gene="znuA"
                     /locus_tag="RH033_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HDV2991507.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETQVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLENVKPLLMKSIHDDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            31007..31762
                     /gene="znuC"
                     /locus_tag="RH033_002332"
     CDS             31007..31762
                     /gene="znuC"
                     /locus_tag="RH033_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HDV2991508.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            31759..32544
                     /gene="znuB"
                     /locus_tag="RH033_002333"
     CDS             31759..32544
                     /gene="znuB"
                     /locus_tag="RH033_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HDV2991509.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMLAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(32689..33699)
                     /gene="ruvB"
                     /locus_tag="RH033_002334"
     CDS             complement(32689..33699)
                     /gene="ruvB"
                     /locus_tag="RH033_002334"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDV2991510.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(33708..34319)
                     /gene="ruvA"
                     /locus_tag="RH033_002335"
     CDS             complement(33708..34319)
                     /gene="ruvA"
                     /locus_tag="RH033_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDV2991511.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
BASE COUNT         8253 a         8901 c         8559 g         8720 t
ORIGIN      
        1 gcgcgtcgat caggacgcgc tttttagtat ttacttcata ttacttcagg tattcacccg
       61 tacgcagagc ttcaatacgt ttatccagcg gcgggtgagt catgaacaac tcactgagcg
      121 atttcgactt accgttaata cagaaagcca tcatgctggt tgcttcttgc ggttcatagc
      181 tggttttcag gcgctgcaat gcagcaatca ttttctcgcg accaaccagt tttgctgaac
      241 ccgcatcggc gtggaattca cgatgacgcg agaaccacat ggtgataatg ctcgccagaa
      301 taccaaacac cagttccaga accgttgcaa ccgcaaagta gatcagcggg ttgccgttgc
      361 tctcttcacc ttcatcacga tttccgccca taaaacccgc ggcaagctgc gccagaatac
      421 gggaaataaa gataacgaag gtgttcacca cgccctgaat cagcgtcatg gtgaccatat
      481 caccattggc gatgtggctg atttcgtgag caattaccgc ctcggcttca tccgggctca
      541 tgttctgcag caaaccggtg ctgacagcaa ccagagaggc atcacggcgc gcaccggttg
      601 caaaagcgtt gatgtccggc gcatggtaga tagccacttg cggcatagcg atccccgcct
      661 gacgagcctg ggttgctaca gtattgacca gccaacgttc cctttcgtta cgcggttgct
      721 cgatcacttc cccgccaaca gatcgtaatg ccatccattt ggacatcaga agcgaaacga
      781 aggaaccacc aaaaccgaac agcaaggcca tgatcatcag cccctgaacg ctgctcgact
      841 gtatccctgt caggctcagt accagcccga aaacgaccat tacggccagg ttcgttagca
      901 ggaagagcgc gattcgcatc ataattttct ttttacctca gtttaacaaa acgcaatatg
      961 cgatacccac atcgtatggg ttacgcgact attttcaagt ctggatagtg cgtaagtcac
     1021 cagaaagaca caactttaca ttttgtagca tctgatttac ggcatcttgc cgctgttaaa
     1081 aaatcaggca caatttcttg tgcctgattg atattacttg acgggagcgg gttgttccgc
     1141 gggtctggct ttttcaagct tcgccagatc gagtgcgata ttcaccgtct catccagata
     1201 aggatccggc tcctggtaat ctttcggtag atcatccagt ttcttcaact ccggtttacc
     1261 ttcgcgttta aagcgttcgt tcaaacgcgc cagacgcgtc gcatcatctt cattattctc
     1321 tttctcacgc acagcgtaat tcagagaaac gatattgcgc ttgtccttca tggcgttgaa
     1381 gcgcgcgata tccttcatga tgttctggaa ctcaggatct ttcgcgatac gcgcattatg
     1441 ttccttcagc agctccggtc caaaggccgt taaatcgcct gatttcacat aggtcgcggc
     1501 atcgatgcta tcccacggca gcgcgttatc ttcgaatttc tcaccggttt ccgtttcttc
     1561 attacccgtc ggcatgatga tgtctggcgt tacgccttta cgttgcgtac tgccgccgtt
     1621 aacgcgatag aatttctgga tcgtgtactg cacagaaccc agcgctggcc attcaggacg
     1681 taacatctga tcgtaaatac ggttcaatga acggtattgc tgaacggtgc ctttaccaaa
     1741 cgtcggttca cccacaacca gcgcacgacc gtaatcctgc attgccgcgg caaagatttc
     1801 tgaagccgaa gcactgaaac ggtcaaccag taccaccagc gggcctttat agaagacctg
     1861 tccgtcggta tcgctgtctt cacgaacttt accgttgtta tcacggacct gaacaatcgg
     1921 gccggatgga ataaacagac cggagagcga taccgcttcg gtcagcgccc caccaccatt
     1981 gctacgcagg tcgatgatga cgctgctgac attctgtttt tccagtttct gcagttgcac
     2041 tttgacatcg tctgtcaaac ccacatagaa gcccggaata tccagcacgc cgactttctc
     2101 tttaccgacg gtcttcaccg acattttaac cgcgcggtct tcgaggcgaa tacgttcacg
     2161 ggtcaacgtt acagtacggg tcttggtccc tttaccagca ggtaaaattt ccagacgaac
     2221 tttactgccc ttcggccctt taattaaggc aaccacatca tcaagacgcc agccaatcac
     2281 gtcaaccatc ggcttgcctg tttgaccaac accgacaatt ttgtcaccaa cgctgatagc
     2341 tttactcttc gctgccgggc ctcctgccac catcgaattg ataacggtgt agtcatcatc
     2401 catttgcagc actgcgccaa taccttccag cgacaaactc atttcagtgt tgaactgttc
     2461 ggtattacgc ggggaaagat agttggtatg cgggtcgatt tcacgcgcaa acgccgtcat
     2521 tgccagcgag aaaacatctt cgctgttggt ttgcgccaga cgacgaatgg caaatttgta
     2581 gcggcgagtc agggtttcac gaatttcttt atccgttttt cctgccagct tcaggcttaa
     2641 ctcgtcgaat ttgactttac tgtcccacag cgcgttcaac tcagcctcgt ttttcggcca
     2701 gggcgctttg ctgcggtcaa ggttataagt gtcgttgccg gtgaaatcca tcggcttttc
     2761 cagtaccgac aaagcgtact ggtaacgttc aaaacgacgc ttttgcgcca gattgtagag
     2821 atcgtagaaa acgtcgagtt tgcctgaacg cagttcatcg cctaactcgg ttttcttttt
     2881 cgcgaactgt tcaacatcgc ttgccaacag cacgttgtgg ctgtaatcga gcagattcag
     2941 gtagcggtca aagattttgg ccgaaaatgt ctgatcgagg tcgaactggc gataatgaga
     3001 acgggtgaag cgcgacgtta cgcgctcact taccgtcgca tgctgcgtct cttcctttaa
     3061 taccggaatt tgatcagcac gcgtgatatc ttctacagcg aaggtctggc ctgctattgc
     3121 aagcaggcca gctaacgcgg taagcctaaa aaacatgttc atgcctggcc cggcctccgt
     3181 ttcagaacac caggtgttct gcgcgcacaa tcaaagacat acccgaattc agctggacgc
     3241 ggacgccgtc tttggtgatt tctaatacgg tggcatccat cgcgttttga cccgctttca
     3301 ccttcagggc ttgtccgaca gtcagagctg aaatgtcaga aaccggggtg tgctgttctt
     3361 cgcgaggtgc ttttactgtt tttggcgctt tctctaccgg cttttgcgca cgaggtttac
     3421 gttcagcgcc ttctttgcgg cgtggcgtgg tcggacgtgg cttgcgttcg cggcgcggtg
     3481 cgtcttcttt ctcaccagca gctgcggcag cttcgcgttt tttcgcttgc tgttcagcac
     3541 gctgtgcctg aacacgcgct ttcgcttctt caagctgctt gcgagcatgc tctacatgtt
     3601 gctcgtccag ctcaccgcat gggttgccgt caagatcgac acgcgttgcg ccgggtttaa
     3661 caccgtaaag ataacgccag ctcgaagtgt agagacgtaa agcggatcgc aattgcgttt
     3721 tgctcaggtt catttccccg gcaacacgat cgaccaaatc ctgaaaaata ccgattttca
     3781 gcggacgcgc ttcaccttcc gcactgaaac agtggggaaa acgttcggcc agaaacgcga
     3841 ttacttcttt actgctattc aacttaggtt gattttccat gaaatttcct gattacaacg
     3901 gacgtagcca acaagcgcag gcatgaacag gcgtcattat aatgacgcta tcagtaaatg
     3961 ctacgttatc cgttgattat cctgcgacgc tcgcaaagaa ttttttgtaa tccgtcgttg
     4021 caagcacttt ttcaagctgt gccacaagct gacgtaagcc ttgctcgtcc tcgtctgtaa
     4081 agcgaccgaa gacggtacta tcgatgtcga gaacaccaat aatctgattt ttcaccacca
     4141 gcggcagaac aatttcagaa ttactcgccg catcacaggc aatatgcccg tcaaacacat
     4201 gaacatcctc gatacgctgc acttgattgc gggcaaccgc agtgccgcac acgccgcgcc
     4261 cgacgggtat ccggacacag gcaattttgc cctgaaatgg tccgagtacc agtgtatcgt
     4321 cctcaagcaa ataaaaacct gcccagttta cgtcagtgag acgctcatat aacaacgcac
     4381 tggtgttcgc aagcgttgcc agaaaactgg tttctcctgc catcagcgcg ttaaagtcgc
     4441 gatttaaatc cgcgtaaaat tctattttgt tcattatgta atcacttggt tgtcttacca
     4501 ggaactgcca gcctattaaa ataagcatta aatgcgttaa tgctcaagat cattccaatc
     4561 atgggttaag attaatgtta attcttatta catttggcac gtcatggctc ttaacacacc
     4621 acaaattacg ccgacaaaaa agataacagt gagggctatc ggtgaggaac tgccgcgtgg
     4681 tgattaccaa cgttgcccgc aatgtgacat gctgtttagc ctgcccgaga taaattctca
     4741 tcaaagtgcc tattgtccgc gctgtcaggc aaaaattcgt gacgggcgcg actggtcgct
     4801 aacgcgcctg gcggcaatgg ccttcaccat gctgttgctg atgccgtttg cctggggcga
     4861 accgctgttg catatctggc tgttaggcat tcgtattgac gccaacgtca tgcaaggcat
     4921 ctggcaaatg accaaacagg gcgatgcgat aacgggatca atggtctttt tttgcgttat
     4981 tggtgccccc ctcattctgg tgacctccat agcttattta tggtttggta accgactggg
     5041 aatgaatcta cgcccggtac tgctgatgct tgagcgactt aaagagtggg taatgctgga
     5101 tatctacctg gtcggcattg gcgttgcttc tataaaggta caagattatg cccatatcca
     5161 ggcgggtgtc ggcttgttct cttttgtggc gttggtgatt ttaacgacgg tgacgttgtc
     5221 acatctcaat gtcgaagaac tgtgggagcg attttatcca cagcgcccca ctacgcgtag
     5281 agacgagaaa ctccgtgtct gtcttgggtg ccattttacc ggctatcctg atcagcgtgg
     5341 tcgctgcccg cgttgccata tcccgctacg cctgcgtcgc cgtcatagtc tgcaaaaatg
     5401 ctgggcggcg ctgttagcgt caatcgtttt gttgttacct gccaacctgt tgcctatttc
     5461 tatcatttat ctgaatggtg gccggcagga agatacgatt ctttccggga ttatgtcgct
     5521 ggcaagtagc aacattgccg ttgcgggaat cgtgtttatc gccagtattc tggtaccgtt
     5581 tactaaagtg atcgtcatgt tcactttact gttgagtatt cattttaaat gccagcaagg
     5641 tttacgcaca cgcattctgt tactgcggat ggtgacctgg attggtcgct ggtcgatgct
     5701 cgacctgttt gtcatatctt taaccatgtc gctgattaat cgcgatcaga tcctcgcttt
     5761 tactatggga ccggctgcgt tttatttcgg cgcagcggta attttgacta ttcttgctgt
     5821 ggaatggctg gacagccgct tactttggga tgcacatgag tcaggaaacg cccgcttcga
     5881 cgactgaagc gcagattaaa aataaacgcc gtatctcacc tttctggctg ctgccgttca
     5941 tcgcgctaat gattgccggt tggctgattt gggatagtta tcaggaccgg ggtaataccg
     6001 tcaccatcga ctttatgtcg gcggatggta ttgtcccggg ccgcacccct gttcgttatc
     6061 agggcgttga agtcggaact gtgcaggata tcagcctgag cgacgatctt cgtaagattg
     6121 aagtcaaggt cagcatcaag tctgatatga aagatgcgct gcgcgaagag acacagttct
     6181 ggctggtgac gccaaaggcc tcactggcag gtgtctccgg gctggatgcc ctcgtcggtg
     6241 gtaactatat cggcatgatg ccaggtaaag gtaaagagca ggatcacttt gtcgcactcg
     6301 atacccaacc gaagtatcgg ctggataatg gcgatctgat gatccacctg caagcccccg
     6361 atctcggttc actgaacagc ggttcattgg tctatttccg taagatcccg gtgggtaaag
     6421 tctacgacta tgccatcaat cccaataagc aaggcgtggt gattgatgtc ctgatcgagc
     6481 ggcgttttac cgacctggtg aaaaaaggta gccgtttctg gaacgtttcc ggcgttgatg
     6541 ccaacgtcag tatcagtggc gcgaaggtga aactggaaag tctggcggca ctggttaacg
     6601 gtgcgattgc cttcgattca cctgaagagt cgaaacctgc cgaggcggaa gatacctttg
     6661 gtctgtatga agatctcgcc cacagccagc gtggcgtaat aataaaactg gaactgccga
     6721 gtggggccgg attaaccgcc gactcgacgc cgttaatgta tcaggggctg gaagtcggac
     6781 agctgactaa actggattta aatcctggtg gtaaagtcac cggagaaatg accgttgatc
     6841 ccagcgtcgt tacgctgtta cgggaaaata cccgcatcga attacgtaat ccgaaattat
     6901 cccttagcga tgccaatctc agcgccctgc tgaccggaaa aacctttgag ttggtgcccg
     6961 gcgatggtga gccacgcaaa gagttcgttg ttgtgccagg cgaaaaagcc ctgctacaag
     7021 agccggatgt tctgacactg accctgacag caccagaaag ttacggtatt gatgctggtc
     7081 agccgctcat tctccacggt gtgcaagtag gccaggttat tgatcgtaaa ctcaccagca
     7141 aaggcgttac ctttaccgtc gccatcgagc ctcagcatcg cgaactggta aaaggcgata
     7201 gcaaatttgt cgtcaacagc cgtgtcgacg tgaaggtggg gctggatggc gttgagtttc
     7261 tcggtgccag cgcctcagaa tggattaacg gcgggatacg tattctgccg ggcgataaag
     7321 gcgagatgaa agccagctat ccactgtatg ccaatctgga aaaagcgctg gagaacagcc
     7381 ttagcgattt acccaccaca accgtaagtt tgagtgcaga gacgctgccg gatgtgcagg
     7441 caggatcggt agtgctgtac cgtaaatttg aagttggtga agttattact gtgcgtccgc
     7501 gagctaacgc gtttgatatc gatctgcata ttaagccgga gtatcgcaac cttctgacca
     7561 gcaacagcgt gttctgggca gaaggtgggg cgaaagttca gctaaatggt agtggtctga
     7621 ccgtacaggc atccccgctc tccagagcat taaagggggc tattagcttc gataacctca
     7681 gcggtgccag cgccagtcag cgtaaaggcg acaagcgtat tctgtatgct tccgaaacag
     7741 cggcccgtgc ggttggcggg caaattacgc ttcacgcttt cgatgccgga aaactggcgg
     7801 tcgggatgcc aattcgctat ctcggtattg atatcgggca aatccagacg ctggatctga
     7861 ttaccgcgcg caatgaagta caggcaaagg cggtacttta cccggaatat gtccagacct
     7921 ttgcacgcgg tggtacacgc ttctcagtgg tcacaccgca aatttcggca gctggcgttg
     7981 agcatcttga tactatcctc cagccgtata tcaacgtcga accaggccgg ggcaatcctc
     8041 ggcgcgactt tgaattacaa gaggctacca taactgattc gcgttacctg gatggcttaa
     8101 gcattattgt tgaagcgccg gaagccggtt cgttaggcat cggtacgcct gtgctgttcc
     8161 gtggtctgga agtcggtacg gttaccggaa tgacgctggg gacattgtca gatcgcgtga
     8221 tgattgcgat gcgcatcagt aaacgctatc aacacctggt gcgtaacaat tccgtcttct
     8281 ggttggcatc gggttacagt ctggactttg gtctgacggg cggcgtagtg aaaaccggca
     8341 cctttaacca gtttatccgt ggcggcatcg ccttcgccac gcctccaggt acgccactgg
     8401 caccgaaagc ccaggaaggc aagcacttcc tgttgcagga aagtgaaccg aaagagtggc
     8461 gtgaatgggg tactgcgctt cccaaataat gcccactgct ccggcgtgcc tgcgccggag
     8521 cgtttatgct aaactgcgcg cctgtttttt tgccagtggt atatgctcgt ggcccaacac
     8581 accgtttatt tcccggacgc ctttctgacg caaatgcgcg aagcgatgcc ttcgacgctc
     8641 tcttttgatg attttcttgc cgcctgtcag cgcccgttgc gccgcagcat tcgcgttaat
     8701 acgctgaaaa tctccgttgc tgatttcctg caattaaccg ctccttatgg ctggacgctt
     8761 acgccaattc cgtggtgtga agaaggtttc tggattgaac gcgacaatga agatgcattg
     8821 ccattgggta gtaccgccga gcatttaagt ggcctgtttt atattcagga agccagttca
     8881 atgttgcctg ttgccgcctt gtttgctgac ggtaatgcac cacagcgggt gatggatgtc
     8941 gctgccgcgc ctggctccaa aacgacgcaa attgccgcgc ggatgaataa cgaaggggca
     9001 atccttgcca atgagttttc cgccagtcgg gtaaaagtgt tacatgccaa tatcagccgc
     9061 tgtggcatca gtaatgttgc gctcacacat tttgatggcc gcgtgtttgg tgcggcagtg
     9121 ccagaaatgt ttgatgccat tttgctggac gctccctgct ccggcgaagg cgtggtgcgt
     9181 aaagatcccg atgcgctaaa aaactggtca ccagaaagca atcaggaaat cgcagctacc
     9241 caacgggaac tgatcgacag cgcctttcat gcattacgcc ctggcggtac gctggtttac
     9301 tcgacctgta ccttaaacca ggaagaaaac gaagccgttt gccggtggct gaaagagact
     9361 taccccgacg cagtagagtt tttaccgctt ggcgatctct tccctggtgc aaacaaggcg
     9421 ctgaccgagg aaggcttttt gcatgttttc ccacaaattt acgactgcga aggcttcttc
     9481 gttgctcgtc tgcgtaaaac tcaggcgatt cccgccttac ccgccccaaa atacaaagtg
     9541 ggcaattttc cgtttagccc ggtgaaagat cgcgaagccg gacaaattcg tcaggcggct
     9601 gcaggtgttg gcttaaactg ggatgaaaac ctgcgactct ggcagcgcga caaagaagtg
     9661 tggttgtttc cggtagacat tgaagccctg atcggtaaag tccgattttc tcgcctgggg
     9721 attaaacttg ccgaaacgca caacaaaggt tatcgctggc agcatgaagc ggttattgcc
     9781 cttgcctccc ccgacaatgt gaacgctttt gaactgacac cgcaggaagc ggaagagtgg
     9841 tatcgcgggc gcgatgttta cccgcaagcc gcgccagtag cggatgatgt attggttact
     9901 ttccagcatc agccgattgg tttagccaaa cgaattggtt cgcgactgaa aaacagctac
     9961 ccgcgtgaac tggtgcgaga cgggaaactt tttaccggta acgcctgaca gcgcacaaaa
    10021 aaagcgcact ttttgactgg cacattcggc tgcctcaact aggctgaaaa atggtgcgat
    10081 cggactggtc gtaccacaat cggcagctaa atggagagca cgaagatgaa aaccagtgtg
    10141 cgcataggcg cttttgaaat cgacgacggc gaattacacg gcgaatcgcc gggtgatcga
    10201 acgttaacca ttccttgtaa atcagacccc gatttatgta tgcaactgga tgcctgggat
    10261 gctgaaacca gtatccctgc cctgctaaat ggcgaacact ctgtccttta ccgtacccgt
    10321 tacgatcaac aatctgatgc ctggattatg cgtcttgcct gatccaaaaa gaacccgtcg
    10381 gcatggcggg ttatttgtcc tggttattcc cccgttgtaa aatctctcct aaacttaacg
    10441 gtacggcacc acacttcggg gatgaaatgt tcgcgctggt actttttgtt tgctacctgg
    10501 atggcggttg tgaagatatt gttgtggata tctacaacac ggaacagcag tgtctctatt
    10561 ctatgagcga tcaacggatc cgtcatggcg gttgttttcc gattgaggat tttatagatg
    10621 gtttctggcg acctgcacag gagtacggtg atttttaatt attgcaattg cacaagagtc
    10681 agttctcccc caaagacagc accggtatca atataatgca ggttgccaat atccacgcga
    10741 tggcgcaaag gtgtatgacc aaaccagaaa tgatcagcac ctgcaattcc ctgccctttt
    10801 tggtgttcac ctaatcgcga gcggctccac aagatctgat gcaaatcgac gtccttttgc
    10861 cattcataaa catcatctgg ataatcggca tgagcaataa catgtttgcc ggtgcgactg
    10921 tgtacttcaa gaataaaggg caaatgctga catttttcca gcgccgtttt cgcttgtttc
    10981 tggtgattat ctgccagcgc aataaaccag tcgccgccat tcatcaacca taaagacatc
    11041 tgccgggatg ccagcgcatc catcgccatc tgttcatgat tgcctcttac cgcacgaacc
    11101 caatgttgtt ccagtaactg caggcaacgt aaactttgcg gcccacgatc gataacgtct
    11161 cccactgaga taagtaaatc tcgccacgga tcaaaacgac aatgccataa tttgcggcgc
    11221 aactgctcaa gacaaccgtg tatatcgcct gaaagccaga tatgtcgcca ttgatgaccc
    11281 gcaattctct gataaacggg cgcaggctgt ttcatcaata ttttcctccc gcgctaaaga
    11341 tcacataatc ttaacaagaa tgttaaaaaa cgctggactc agacagtaga gtgtgtgtta
    11401 tggttgacta taaagtcagc gaagggaatg cttctggctt ttaacagata aaaagagacc
    11461 gaacacgatt cctgtattcg gtccagggaa atggctcttg ggagagagcc gtgcgctaaa
    11521 agttggcatt aatgcaggct tagttgcctt gctctttaag aatagatgac gacgccaggt
    11581 tttccagttt gcgtgcaaaa tggtcaataa aaagcgtggt ggtcatcagc ttaaatgtta
    11641 aaaaccgccc gttctggtga aagaactgag gcggtttttt attggaaatc aaaaggctat
    11701 tttaggtaat taacagagtt tttcagctcg ctctataaac ggtgccagac tcattttttc
    11761 gccgggattg ttaggatcat caatctgaat caccgaaatg ggttgggctt tagtcttccc
    11821 actggcaact tccttttgtg cgatatcgtt taaaggatac tgcacgaggg tacttgggtt
    11881 gatgacatac aaagcattac ccggtcggca agtcagcatc acctcttcgc gattaaacgc
    11941 ccatttgtct ttacccactt caaaacggct gacggtaatc acctgcggtg ccgccagcgc
    12001 cgctgcagaa ctggtgagta acagaaacgc cagaatactt tttttcatca ttttcgtaat
    12061 ccatcaaaaa ggttcgagag tcgcaagcag gctgatgatc accagcacta ccgcacctat
    12121 cgcccattcg agtttagtca tccacacaaa ccagggagcc gcacgatctt catcctgcct
    12181 catgcgcggt acaagaacat aacgattagc cagcgcgatg accaccataa tcatcaccag
    12241 gatggctttg agcattaaga gttggcccca gtaagtcgta agcgtggggg gaaatccggt
    12301 aatcaacaat gcattaagca cgccgcttgc cagtacgccg atcaccgcaa aatgcccgca
    12361 ccaggaaaaa cgcatcagcg cctgaatagc ctggtgtcgc caacggcctt tgatgagttg
    12421 catacaccag agcaccggca gtaatccccc aaaccacgcc gccgcacaga tcaggtgaat
    12481 tgcgtgatta gtctgatgga ttttcgccgt taccccttca ttcagcgtcg catgcccgac
    12541 tcccgccagc agaataaatt gcgcggtagt gagcataaat agcaaacgtg gcatattctg
    12601 cggttgcata agggcaacga tcaacgttac gagggcgaga acaatttgcc ataaccagat
    12661 gccaccaaac tgcgtttgca aaacagccag ccaaatgttt ggcgaaaata catctgacca
    12721 tcccgttccc atcaacccgc cctgaacggc gagcattgca gtggcactga tcaaactcca
    12781 gacggcagcg tgctgttgta aacgcagaaa acgcttcgtc agcaagcgac gaatcgttaa
    12841 gggggccagc caggcaccgt acattgcgaa gccaaaaacc agcatcaacg aggtgaaatg
    12901 gataaatcgc agcgcgatcc aggtaaacgc cagcatgatt tatttcacgc taaaggtgta
    12961 gtgtcctttc gttttatgcc cgtccaccga aacaacatgc cagtcaacgg tgtaggttcc
    13021 gggtttcagc gaatcggcca gtggaacaat cagttgcttt tggtcctgct catttcgctt
    13081 cgccggtaat gttttaatat tttcgttttt tggccccgtg atttttgcac cactgaatcc
    13141 tgtttcaacg ccttccgaga agtttaaagt gattgcctgc ggggcagctg tcacttgcgc
    13201 atttgctgca ggatactgat gcgttaaatg cgcatgtgcc cagacggaag gtgtcaccaa
    13261 cgaagtggtc aggattgcca gcgcgtagcg aagggagcgt gcagttgaag ccatattatc
    13321 tattcctttt tgtaataact tttttacaga gcataacctt gtctaatgtc tgagtcgagg
    13381 atcatcaatt ccggcttgcc atccaggctc actcttagta acttttgccc gcgaatgatg
    13441 aggagattaa gaatgctgaa gaatctggct aaactggatc aaacagaaat ggataaagtg
    13501 aatgtcgatt tggcggcggc cggggtggca tttaaagaac gctacaatat gccggtgatc
    13561 gctgaagcgg ttgaacgtga acagcctgaa catttgcgca actggtttcg cgagcggctt
    13621 atcgcccacc gtttggcttc ggtcaatctg tcacgtttac cttacgagcc caaacttaaa
    13681 taaaacttat acagagttac actttcttac ataacgcctg ctaaattatg agtattttct
    13741 aaaccgcacc cataatttgc agtcattttg aaaaggaagt cattatgtcg ttctggaaag
    13801 ttgcagcagc gcaatatgag cctcgtaaaa cctcactgac cgaacaggta gctcatcatc
    13861 tggagtttgt gagtgccgcc gccaggcaac aatgtcagct attagttttt ccatcgcttt
    13921 ctttactggg gtgtgattat tcgcgacgtg cccttcctgc cccacccgat ctctcgctgt
    13981 tagacccgct ttgttatgct gcaacgacct ggcgaatgac catcattgcc ggccttcccg
    14041 ttgaatataa cgatcgcttt attcgtggaa ttgcggtgtt cgcaccgtgg cgaaaaacac
    14101 cagggatcta ccatcagagt catggtgcat gtctgggccg tcgttccaga accatcacgg
    14161 tggtcgatga acaaccggaa ggtatggata tggacccgac ctgttcactg tttacgacag
    14221 ggcaatgtct gggggaaccc gacctactgg catctacccg ccgcttacaa tttttttcac
    14281 atcagtactc aattgccgtg ctgatggcaa atgcccgtgg taacagtgca ctatgggatg
    14341 aacatggtcg tctcatcgtt cgcgccgatc gtggttcatt attgttagtc ggtcagcgtt
    14401 catcacaggg ttggcaaggc gatatcattc cattacgcta ggctttttcg gcctggagca
    14461 tgccatgttg cgcattatcg atacagaaac ctgcggtttg cagggaggga tcgttgagat
    14521 tgcctctgtt gatgtcattg acggaaaaat cgtcaacccc atgagccacc tggtgcgccc
    14581 cgatcgtcct attagtccac aagcgatggc gattcatcgc atcaccgaag ccatggtcgc
    14641 cgataaaccg tggattgaag atgtgatccc acactattac ggtagtgaat ggtatgtcgc
    14701 gcacaacgcc agctttgacc gccgcgtact gcctgagatg cccggtgagt ggatttgcac
    14761 tatgaaactg gcccgtcgtt tgtggccagg gatcaagtac agcaatatgg cgttatataa
    14821 aacacggaag ctcaatgtac agacgccgcc gggcctgcat catcaccgcg cgttgtatga
    14881 ctgttatatc accgctgcgt tgcttatcga tattatgaac acctccggct ggacggcaga
    14941 acagatggcc gatatcaccg gacgtccgtc gttgatgacg accttcacct ttggcaaata
    15001 ccgtggcaaa gcggtttccg acgttgccga acgcgatccg ggctatctgc gctggttgtt
    15061 taataacctg gacagcatga gcccggagct gcgtttaaca ctgaaacatt atctggaaaa
    15121 tacttaatcc tcaggcgtag cgggtaatgt tccctgcgcc agcgcgacca gaaaagcata
    15181 ttccatcgct acgccttcgt acgatttaaa gcgaccagat ttaccgccat gacctgagtc
    15241 catgtcggta cagagcaata aaagatggtc atcggttttc agctcgcgca atttagcgac
    15301 ccattttgcc ggttcccaat attgcacctg agaatcgtgc aaaccggtcg ttaccagtaa
    15361 atgcggataa gcctgtgcgg tgacgttgtc atatgggctg tagcttttca tgtactcgta
    15421 atattgcgga tcctgcggat tcccccactc ttcaaactca ccagtggtaa gaggaattga
    15481 ttcatcaagc attgttgtta caacatcaac aaacggtacc tgggcgataa cgccgtggaa
    15541 taattccggg cgttgattaa ttgcaacgcc catcaacatg ccccccgcac ttccgcccat
    15601 cgcataacag agcgaaggag agccatagcc cagttttaac aatgcatcgc aggcatcaag
    15661 ataatcatta aacgtatttt tcttcttcag aaatttaccg tcttcgtacc attgttgccc
    15721 cagctcacca ccgccgcgaa catggacaat ggcgtagaca aagccacgat ctaacaaact
    15781 caagcggcta aaactgaaat cggcatcaat acttgcgccg taagaaccgt agccatacac
    15841 cagtagcggg ttgtgtcctt tgcgaaaatg tttgcgatga tagaccagcg aaaccggaac
    15901 ttcgacgcca tcacgggcga ctatccacag gtgttcactg cggtaattcg ccgcatcaaa
    15961 accaggaact tccgtttgtt ttaatacacg acgctcaccg gtatccatat ccagttcaaa
    16021 caaagtatct ggtgtggtca tggaagaata accataacgc aatcgcgcgg tttcaggttc
    16081 tggattgtag gcaatccagg tcacataggc cggatcatca aaggcaatgc caatgacttc
    16141 ccgggtcttg cggttgattt ggcgcaaact ggttaacccg cgctgacgct cttcaaccac
    16201 cagccagtcg gtaaacagcg taaacccttc cagcataatg ttatcacgtg gcggaattaa
    16261 ctcttcccac tgttgctcat cacgcatacg ggtacggtat aagccaaagt ttttaccgtg
    16321 gcggttggaa cgcagataaa accgatgctg gtagtgatca aggctgtatt cgtgatcttt
    16381 gcggcgaggc agaaaaacaa acggctcggc atcggccatt tccgcgtcca gcaggcgaac
    16441 ttcactggtg gtggcgctgg ccagatgaat gaccacataa tgcttcgagg tcgttttatg
    16501 caggctgacg taataggtat cgtctttttc ttcgtaaatc agtttatctt gcgatgctgg
    16561 cgtaccgatg gcgtgacgcc agacctgata aggcagcagc gtcactggat gcttgcgaac
    16621 atagtagaaa gtccaggagt catttgccca gacaaagctg ggttcaacgt tatccagcag
    16681 ttccgggtac cagttacctg tttccagatt acgaaaacga atgccatact gccgtcggga
    16741 aagaaaatct tctgccagtg ccataatggt gttatctggc gtaatcgcca ttccgcccat
    16801 cgaataaaac tcactatgag ccgcgcgctt gttggcatcg agcaatgttt cccactcgtc
    16861 ccactcttca ctgaacgccg attgacgctg gtagatagca tattcacagc ctggttcata
    16921 aatatgccga tagcggtagc catttttgat gtagggcgca gaaacttctc gttgcggaat
    16981 gcggtcgatg atttccttta agatgcgatc ctgcaaggct tgttgcgagg ccatcacccg
    17041 atgaccgtaa ctattttctt gttgcagata gtccaggact ttcggctgag aacgcgtatc
    17101 gtcccgcagc cagtagtaat tatcgatgcg cgtatcgcca tgaagcgtca tggcgtgggg
    17161 aatgcgggcg gcttttggta gcatgttatt gttctttctg gttgaaacat cttataaggg
    17221 tggcaaaact caccggggat gcaagcgaaa cagggtagtc attgcttaga tgatgacagg
    17281 taatggcgcg gatatcgaat gttatgcaga cagagaaaat cagcctgctc aaatggctgt
    17341 gcggttctgg ataacccgaa ataatcagct tcaggctatc cagagagcgg aattactccg
    17401 ccaaagtacg tttttgctgt tcgagatcgc gttcaatgcc ttcacgaaca tcctggggga
    17461 ttttcagcgc atcacccagt gcattcaggt aactgcgttc cataaaatgg tcaatatcaa
    17521 tagccgcgca gctcaggaaa tagatttcca gcgcctcttc ttcattgcgg accccggtag
    17581 ccaggcgttg tggatccagc ggttgttcga ttgcctgctc aatgagtaca cgccccttct
    17641 cttccacacc ggcttctcgc aattgctggt cgattgccgc acgttctttg gcatcaatat
    17701 ggccatcact tttagcggca aagaccagcg caaggatcaa acgttccgta cgctcatcca
    17761 gcggcgtact ttgcgcgcca aactgcggtt cgtcctgatg cgccgcgcga attttatctt
    17821 tgtatttatt ccacagcacc gtacccgcga ccgctccgcc gccaaccagt aacgcgttgg
    17881 tgccatattt cgtaagaagt ttccgtgctg atttatttgc gaccagcagc ccagccagtc
    17941 cgcctaatgc gccaggcact aaaagtttgc ccaatccctg atccgcagac gaggaggtag
    18001 aagaacggct ttgcccaagc agggattgca gttgatttaa ccagttagcc atatttgctc
    18061 ctcaataacc atttttagtg cctgtagcat agcggatgag gatgtcagaa aatgtatatt
    18121 gagacaaaag atgcgcaaat aagcggggcg gtagaccgtt attcaaagaa attcgcgcca
    18181 ggtaaggtgt ttactgtata tcaggtgata agggtatttt aggtgaaagt agtttgacgt
    18241 tttgagtggc actgtttgac gctgattgcc cgatgcgacg ctgttgcgtc ttctcaggcc
    18301 tacgattgtg gtgcgttgtg gggcggacaa ggcattcgcg ccgcatccgc cgacggtcaa
    18361 tcgaattatc agaggccatt aacgccgctg atattccgcc attcccgctt tgcagtccac
    18421 gctgacctgg taatgaatat cggcactttt accacgtacg gttagcggta ctgaccattt
    18481 atcatcttta ccctgaatgt cctgcaaact gacccatgcc acaggatcgg cctgaccgac
    18541 aattttttga tcatctgccc agcgcgccac gcgattttgt tgataatcac gttttacgct
    18601 cgcggcaatt ccggcagcat ccagaccttc acactttggg aaagtgaccg acttgctggt
    18661 ttcattattg gcagcgaaaa ctgatgcgca ggcagaaacc aacaacagcc ctaaaaacgc
    18721 ccctcttttt ttcatgtttt tctccatagc acaatgattc aggagaaagc atggtacaaa
    18781 ttgtcaggag cgcaagttgc ttcaggccgc gtgtgaggca tcttcctctt cggattcagc
    18841 accgacgacc tgtggggacg gtaatttgcc ggttttcaga atggtgctca gcacatcttt
    18901 ttgttctgcc agccatagcg aaagggcttc acgttgctcg tcttccattt gcactggaga
    18961 atttgtcagc caggtgtcaa gcagatccgc cgtatcaagc attttgtcat aagcatcggc
    19021 ttcctttttg ctggtaaacg acattttctc ttcgccctca cgaatgacta cgtatttaac
    19081 ttcaaccgcc atttgcagcc tctcataata actgtgattt tatacagtat atttcttctc
    19141 gattgggaaa tcaacatcaa caataaagac acgcgcaaac gttttcgttt atactgcgcg
    19201 cggaataaat caggggatat tcgttatgac gttattaggc actgcgctgc gtccggcagc
    19261 aactcgcgtg atgttattag gctccggtga actgggtaaa gaagtggcaa tcgagtgtca
    19321 gcgtctcggc gtagaggtga ttgccgtcga tcgctatgcc gacgcaccag ctatgcatgt
    19381 cgcgcatcgc tcccatgtca ttaatatgct tgatggtgat gcattacgcc gtgtggttga
    19441 actggaaaaa ccacattata tcgtgccgga gatcgaagct attgccaccg atatgctgat
    19501 ccaacttgaa gaggaaggac tgaatgttgt cccctgcgct cgcgcaacga aattaacgat
    19561 gaatcgcgag ggtatccgtc gcctggcagc agaagagctg cagctgccca cttccactta
    19621 tcgttttgcc gataacgaaa accttttccg cgaggcggtt gctgaaatcg gctatccctg
    19681 cattgttaaa ccggtgatga gttcctccgg caaggggcag acgtttattc gttctgcaga
    19741 gcaacttgct catgcatggg agtacgctca gcaaggcggt cgcgccggag cgggccgcgt
    19801 aattgttgaa ggcgtcgtta agtttgactt cgaaattacc ctgctaaccg tcagcgcggt
    19861 ggatggcgtc catttctgtg caccggtagg tcatcgccag gaagatggcg actatcgtga
    19921 atcctggcaa ccacagcaaa tgagccagct ggctttggaa cgtgcgcaag agattgcccg
    19981 taaagtggtg ctggcactgg gcggttatgg gctgtttggt gtcgagctgt ttgtctgtgg
    20041 tgatgaggtg attttcagtg aggtctcccc tcgcccacat gataccggga tggtgacgtt
    20101 aatttctcag gatctctcgg agtttgccct gcatgtgcga gcctttctcg gacttccggt
    20161 tggcgggatc cgtcagtatg gtcctgcagc ttctgccgtt atcctgccac aactgaccag
    20221 tcagaatgtc acgtttgata atgtgcagaa tgccgtaggc gcaggcatac agattcgttt
    20281 atttggtaag ccggaaattg atggcagccg tcgtctgggt gttgcgctgg cgaccgctga
    20341 gagtgttgat gaagcgattg agcgcgcaaa gcacgccgcc ggacaggtaa aagtacaggg
    20401 ttaaacccgg caaaaaaacg ctacaaaaat gcccgatcct cgatcgggca ttttgacttt
    20461 tacagcttag cgccttctac agcttcacgc gccagcttag taatgcggtc gtaatcgccc
    20521 gcttccagcg catctgccgg aaccagccag gaaccaccga tgcacagcac gcttttcagc
    20581 gccaggtagt cacggtagtt agccggagaa ataccacctg tcgggcagaa acggacctgg
    20641 gagaacggac ccgcgatcgc ctgcagggct ttcacaccgc cgttagcttc agccgggaag
    20701 aatttgaact ctttcaaacc gtagtccata cccagcatca gttcggaaac agtgctgatc
    20761 cccggaatca gaggaatagt cccttcggta gcagctttca gcagcggctc ggtcagaccc
    20821 gggctaattg caaactgtgc acccgcttca gtgacttctg ccagctgctg tggattcagc
    20881 accgtaccgg cacccacaat cgcttcaggc acttctttgg cgatagcacg gatagcgtca
    20941 actgcgcact cggtacgcag agtcacttcc agaacgcgca ccccaccagc aaccaacgct
    21001 tttgccatcg gcaccgcgtg ttccagtttt tttaccacga taaccggtac aaccgggccg
    21061 gtggtcagga ttgattctgc acttgttttc cagtttttca tcagagtttt ctctcgcctg
    21121 attacaaatt tgtcgtctta aaaagtgata caggttgcgc cctgttcggc accggacagt
    21181 ttttcacgca aggcgctgaa taattcacgt cctgttccca cgcgtgacgc gctcaggtca
    21241 ggaatgtgtg gttcgcgagc agccagttcc gcttcgtcta ccagcagcgt cagttcgcct
    21301 gtctgtccat tcactcgaat gatgtccccg tcgcgcactt ttgccagcag cccgccatcg
    21361 taggcctctg gtgttacgtg gatagctgac ggcactttac ctgaagcgcc ggagagtcgt
    21421 ccatcggtaa ctaacgcaat tttgaaacac cggtccaata atacaccaag tggcggcatg
    21481 agtttatgta attctggcat tccgttcgct tttggcccct gatgacggac aacaacgaca
    21541 caatcgcggt ccagtaaacc cgcttcaaag gccggcataa cgtcatgctg gctttcaaaa
    21601 acaaccgctg gcgcttcaat cacctggttc tctaccggca cggcagaggt tttcataacc
    21661 gctcggccca ggttaccgct taacactttt gtcccaccat gatgagagaa aggttgttcg
    21721 aaggacgcga tcacattgtt gtcgagtgat ttttccgccc cttcccgcca gtccagttcg
    21781 ccattattca gccatggttc aagggtataa cgagacagac caaagcctgc caccgtattg
    21841 acatcttcat gcagcaggcc tgctttgagc agttcacgca ccagaaccgg tacgccacct
    21901 gccgcctgga agtggttaat atcggccgga ccgttcgggt agagacgtgc catcagcggt
    21961 acaacatcag aaaggtcaga gaagtcatcc cagttaatct gaataccggc tgcgcgcgcc
    22021 atcgccacca ggtgcatggt gtggttagtg gaaccaccgg tcgccagcag tgcaacgata
    22081 ccgttcacca ccactttctc atcgatcatc ttaccgatcg gcatccattc attaccatta
    22141 ccggtcatgc gcgtaacctg gcgcgcagct gcagcggtca aagcatcgcg cagcggagaa
    22201 tccggatgaa caaaagaaga gcctggcaac tgcatcccca taaactccac caccatctgg
    22261 ttggtattgg cagtaccgta gaaagtacat gttcccggcg catgataaga cgcggcttct
    22321 gactccagta gtgccatgcg gtccacttta ccttcggcat aaagctggcg aatacgcact
    22381 ttttctttat ttggtaaacc gcttgccatc ggtccagacg gcacaaacac cgcaggcaaa
    22441 tgaccaaacg acagggctgc catggtcaga cccgggacaa tcttgtcgca cacaccgagg
    22501 aacagcgcac catcaaacat gttatgggac agccccaccg ccgcagacat cgcaatcact
    22561 tcgcggctta acagcgacaa ttccattcca tcctgcccct gggtgacacc atcacacatc
    22621 gccggaacac cgcccgcaac ctgaccaacc gcattcgctt catgcagggc tttacgaatg
    22681 atttctggat agtgttcata aggctggtga gcggagagca tgtcgttata ggaggtgatg
    22741 atggcgatat tgttacgcaa catgcttttc aaagaggctt tgtcttctgg ctggcaggca
    22801 gcgaaaccgt gtgccaggtt accgcatgcc aactgcgaac gatgaacggt cgaagttttc
    22861 gcttgttcta tccgggcgag ataagcagag cgagtctcgc gcgaacgttc aatgattcga
    22921 tttgttacgc gtaacaattg tggattcata aaggctcctg aaattgagtt gtcagagcag
    22981 gatgattcac aacacgtttc attcagagga ttcatgactg aaacgcctgt aaccggagct
    23041 catagggcaa aaacgtttca gtcagtgtaa taaaaaaagc ctcgtgggtg aatccgcacg
    23101 aggcctgaaa gtgtaaaaat tgttctacaa tctgtgcaag atcatgttac cggtaaaata
    23161 accataaagg atgagcgcag atattactca aactcattcc aggaacgacc atcacgggta
    23221 atcatcgcca ctgaggcaac gggtccccag gttccggcct gatacggttt cggcgcatca
    23281 ttgtccatcg cccacgcctc agtaatggag tctacccatt tccaggcttc ttccacttca
    23341 tcgcgacgta cgaacagtgc ctgaatacca cgcatggttt ccagcagcag acgttcatag
    23401 gcgtctgcca gatgcgtttg attaaaggtt tctgaatagc tcagatccag cttggtgatt
    23461 tgcaggttat gtttatggtc aaggccagga actttattta gtacctggat atccacgcct
    23521 tcatcaggtt gcagacggat agtcagttta ttctgcggca gatcctgcca cgactcttta
    23581 aacagattca gttcaggtgt tttgaaatag accacgactt cagaacattt ggtcggcaga
    23641 cgtttaccag tacgcaggta gaatggcaca ccggcccagc gccagttatc aatgtcgacg
    23701 cggatcgcca cgaaggtttc tgtattgctg ctcttgttcg cgccctcttc ttccagatat
    23761 cccggcactt ttttgccctg ggcgaagccc gcagtatatt gcccgcgtac ggttttttcg
    23821 cgcacgttgg agcggtcaat gcggcgcaga gacttcagta ctttcacttt ttcatcgcgg
    23881 atgctgtctg cactcaggtc agacggcgga gacatcgcaa tcatgcaaag aatttgcagc
    23941 aggtggttct ggatcatgtc gcgcatctga ccggctttat caaaatagcc ccagcgccct
    24001 tcgatcccca cttcttctgc cacggtaatc tcaacatgat caatggtgcg attgtcccag
    24061 ttattcacaa acagggagtt agcaaaacgc agcgccaaca ggttcagcac cgtttcttta
    24121 ccaagatagt ggtcgatacg gtaaacctgg cactcctcga agtattcgcc aacctgatcg
    24181 ttgatttcct gcgaggtcgc cagcgacgtc cccagcggtt tctccatgac tacgcgtgcc
    24241 ggtttagcat tcagttttgc ctcaccaagc cctttgcaaa ttgcgccaaa agtgctgggc
    24301 ggcatggcaa agtagttaat ggtgatacga tttttttgat ccagcatcgc gccgagacgg
    24361 ctgaatgcag cagtgtcatt gacatcgaga ttacaaaaat ccagacgtgc actcagggtg
    24421 tcccataaac cttcatcaat ggtttctttc atgaaagttt cgagcgcctc gcggacaact
    24481 ttggtataag ccgctttatc ccagtcagca cgccctacgc cgataatccg ggtgtccggg
    24541 ttgagctgac cggctttttc cagttgatac agggaaggca gcaatttacg acgcgcaagg
    24601 tcgcctttcg cgccgaaaat gaccaggtca caggcctggg ctgtttgcgt taccgccatg
    24661 tcattctcct taagttaact atcccggtac ttaagccagg gtatacttgt aattttctta
    24721 cggtgcactg tactgctttt acgagcttgc gaaaactgta aacgcttatc cacccgtgcg
    24781 attacggtaa aagcgcgcaa agtgcggcaa aactgataaa aaaatcatcg ttttccattg
    24841 ggtaaaaatc tgacactgat catgttatga aaaaaaataa caactttttt atctgctttt
    24901 gtcattaaca cagcacacag gcgtaatatc tgacaaaact gcatttcgat ttctttcaga
    24961 gcggaaatcg tcattacccg tgagtctctt tacatcatga atatgctgga aaaaatccag
    25021 tctcagctgg aacatttgag caaatctgag cgcaaagttg ccgaggtcat tctggcttcg
    25081 cccgataacg cgatccattc gagtattgct gctctggcgc tggaagccaa tgttagcgaa
    25141 ccgacggtga atcgtttctg tcgcagcatg gacactcgcg gttttcctga ttttaaactt
    25201 catctggcac aaagtctggc gaatggcact ccctatgtta atcgcaacgt caatgaagat
    25261 gacagcgttg aatcatacac ggggaaaata tttgagtccg caatggcaac gctcgatcat
    25321 gtccgtcatt cactggataa atctgccatc aaccgcgccg tcgatttgct cactcaggca
    25381 aaaaaaatcg cctttttcgg attaggctct tcagccgccg ttgcccacga tgcgatgaat
    25441 aagttctttc gttttaatgt tccggtggtg tactccgatg atatcgtgct gcaacgcatg
    25501 agttgtatga attgtagcga cggagacgtg gtggtgctga tttctcacac gggaagaaca
    25561 aaaaatctgg tcgagctggc gcagctggca cgcgaaaacg acgccatggt gattgccctc
    25621 acctctgcgg gtaccccgct cgcccgggaa gcaacgctgg caattaccct cgacgtaccg
    25681 gaagatactg acatttatat gcccatggtt tctcgacttg cgcagctgac cgtgatagat
    25741 gtgctggcga caggatttac tttgcgacgc ggtgcaaaat tcagagataa cttgaagcgg
    25801 gtcaaggaag cgctgaagga atcgcgtttt gataagccgt tacttaattt aagtgacgat
    25861 cgctaaaaac gactgtcact gtcctaatct tatacgacat ccgaatgaga ttaatttatc
    25921 gccatcgcgg cgttatttca ttcggatttc atgttcaagc aacacctggt tgtttcagtc
    25981 aacggagtat tacatgtcca gaaggcttcg cagaacaaaa atcgttacca cgttaggccc
    26041 agcaacagat cgcgataata atcttgaaaa agttatcgca gcgggtgcca acgttgtacg
    26101 tatgaacttt tctcacggct cgcctgaaga tcacaaaatg cgcgcggata aagttcgtga
    26161 aattgccgca aaactggggc gtcatgtggc tattctgggt gacctccagg ggcccaaaat
    26221 tcgtgtatcc acctttaaag aaggcaaagt tttcctcaat attggggata aattcctgct
    26281 cgacgccaac ctgggtaaag gtgaaggcga caaagaaaaa gtcggtatcg actacaaagg
    26341 cctgcctgct gacgtcgtgc ctggtgacat cctgctgctg gacgatggtc gcgttcagtt
    26401 aaaagtactg gaagttcagg gcatgaaagt gttcaccgaa gtgaccgtcg gtggtcccct
    26461 ctccaacaat aaaggtatca acaaacttgg cggcggtttg tcggctgaag cgctgaccga
    26521 aaaagacaaa gcagacatta agactgcagc gttaattggc gtagattacc tggctgtctc
    26581 cttcccacgc tgcggcgaag atctgaacta tgcccgtcgg ctggcacgcg atgcaggatg
    26641 tgatgcgaaa attgttgcca aggttgaacg tgcggaagcc gtttgcagcc aggatgcaat
    26701 ggatgacatc atcctcgcct ctgacgtggt aatggttgct cgcggcgacc tcggtgtgga
    26761 aattggcgat ccggaactgg tcggcattca gaaagcgttg atccgtcgtg cgcgtcagct
    26821 taaccgcgca gtaatcacgg cgacccagat gatggagtca atgattacta acccgatgcc
    26881 gacgcgtgca gaagtcatgg acgtagcaaa cgccgttctg gatggtactg acgctgtgat
    26941 gctttctgca gaaactgctg ctggtcagta tccgtcagaa accgttgcag ccatggcacg
    27001 cgtttgcctg ggggcggaaa aaatcccgag catcaacgtt tctaaacacc gtctggacgt
    27061 tcagttcgac aatgtggaag aagccattgc catgtcagca atgtacgcgg ctaaccacct
    27121 gaaaggcgtt acggcgatca tcaccatgac cgaatcgggt cgtaccgcgc tgatgacctc
    27181 ccgtatcagc tctggtctgc caattttcgc catgtcacgc catgaacgta cgctgaacct
    27241 gactgctctc tatcgcggcg ttacgccggt gcactttgat agcgctaatg acggcgtagc
    27301 ggctgccagc gaagcggtta atctgctgcg cgataaaggt tacttgatgt ctggtgacct
    27361 ggtgattgtc acccagggcg acgtgatgag taccgtgggt tctactaata ccacgcgtat
    27421 tttaacggta gagtaagtac gttgccggat gcggcgaaaa cgcctcatcc ggcctacagt
    27481 tcaatgataa ttcaacagat ttcgaatctt ctgaagcaaa cttgaactta tcatcagtcg
    27541 aaggcctctc ctcgcgagag gcatttttta tttgatggga taaagatctt tgcgcttata
    27601 cggttggatt tcgcccggtt tgcgagtttt cagcaatttt aatatccagg tgtattgttc
    27661 tggtcgcgga ccaacaaaaa tctcgacttc ttcattcatc cgccgcgcaa tcgtatgatc
    27721 atccgcctct aacagatcat ccatcggtgg gcgcacctga atcgtcagac gatgcgtctt
    27781 gccatcataa atcggaaata gcggtacaac gcgcgcacgg cacactttca tcaaacgacc
    27841 aatcgcgggc aacgtcgctt tataggtggc aaagaaatca acaaattcgc tgtgttctgg
    27901 gccatgatcc tgatcgggta aataatatcc ccagtaaccc tgacgtaccg actggatgaa
    27961 tggtttaata ccatcatttc tcgcatgcag acgaccacca aagcgacggc gcaccgtgtt
    28021 ccagacataa tcaaaaaccg ggttgccctg attatggaac atcgctgcca ttttctgccc
    28081 ttgcgaggcc atcagcatgg caggaatatc gacggcccaa ccgtgcggca ccagaaaaat
    28141 cactttctcg ttattacgcc gcatctcttc gatgatctcc agcccttgcc agtcaacgcg
    28201 cggctgaatt ttctccggcc cgcgtattgc caactcagcc atcattgcca tcgcttgcgg
    28261 cgcagtggca aacatctcat caacaatcgc ttcgcgttca gcttcactac gttctggaaa
    28321 gcagagcgac agattgatta acgcacgacg gcgtgagctt tttcccagtc gtccggcaaa
    28381 acgtcccagc cgtgccagaa tgggatcacg gaactttggc ggcgttaaag cgatacccgc
    28441 catcgctgct acgcccagcc atgctcccca gtagcgcggg tggcgaaagg atttatcaaa
    28501 ctcaggaatg tattcgctat tatttttttt cgtttccatg cttttccagt ttcggataag
    28561 gcaaaaatca atctggtgat agtgtagcgg cgcaacttgc cccgcaccaa ataaaaaagc
    28621 cggtactgac tgcgtaccgg ctgcgaatgg atgttaatta atcaaaccgt agctgcggca
    28681 caatctcttt ggcctgcgcc aggaattcgc gacgatcgga gccggtcagc ccttcggtac
    28741 gcggcagttt tgccgtcagc gggtttacgg cctgctggtt tatccatact tcatagtgca
    28801 gatgcggccc ggttgaacga ccggtattac cggaaagcgc gatacggtcg ccacgtttca
    28861 ccttctgtcc cggtttcacc agaatcttgc gcaagtgcat ataacgcgtg gtgtagctgc
    28921 gaccatgacg aatagccaca taataacctg ctgcgccact gcgtttggca accaccactt
    28981 caccgtcacc cactgaaagc actggcgtac cctgcggcat ggcgaaatca acacctctgt
    29041 gtggcgcaac gcggccggtc accggattag tacgacgcgg gttaaagtta gacgagatac
    29101 ggaactgttt cgccgtcggg aatcgcaaga atcctttcgc cagaccagta ccgttacgat
    29161 cgtagaattt gccatcctca gcgcggattg cgtaataatc tttaccttct gaacgcaaac
    29221 gtacgcccag cagctggctt tgctcacgtt taccatcaag catttctcga gacattaaca
    29281 ccgcaaattc atcgcctttt ttcagtttgc ggaaatccat ttgccactgc atggctttaa
    29341 tcactgcgct cacttcggcg ctggttaaac cggcgtttct ggcgctggca acaaagctcc
    29401 ccccgacggt acctttcagc agattgttga cccactctcc ttgctgcatt tcgctggtca
    29461 ttttaaaacc gttagcggca gtacggtcat aggttcgggt ttcacgacga gacacttccc
    29521 aggtgaggcg ctgcagttcg ccgtccgcgg ttaatgtcca ggagagttgt tgaccgattt
    29581 tcaggttacg caattctttg tcggccgcag ccagttgggt gatatcaccc atatcaatac
    29641 catactgatt gagaatgctg cttagcgtat cgccagtgga aacaacatat tcatgcacgc
    29701 ccgcttcacc ggcgattttg tcatccagtt cgtcctgggg aatggcttca tcttcttgtg
    29761 cagcttgatc aatcggctca ctggcttcag gtaagagcga acgaatttcg ttctgttcca
    29821 gctcaatggt tttgacaatt ggcgtggcat cgcggtgata aacatagggc cgccagacag
    29881 cgacggccag agtaagaacg gtgagcgacc ccaacataac gcggtgtggt cgcggtaaat
    29941 tattaaacgc cagggcgaca gagcgggcta tctgttgcac gtaatcactt cctcattaat
    30001 ctcctttcag gcagctcgca tactggttgg ctaattgact caggaattct gaatagcttg
    30061 ttttacccag tttgatattc gtccccaggg gatccaacgt tcccatacga acggatgtcc
    30121 ctcgtgcgac gctctcaacg accgctggcc tgaactgtgg ctcagcaaaa acgcaggttg
    30181 ctttttgctc aaccaactgt gttcttattt catgtaaacg ctgcgcgcca ggttgaatct
    30241 cagggttaac ggtaaaatga ccaagcggtg tcagtccgaa ctgtttttcg aaatagccgt
    30301 aagcatcgtg aaaaacgaaa taacctttcc ccttgagcgg cgcgagctcg ttaccaacct
    30361 gcgtttcggt tgaggctaat tgtgcctcaa aatccttcag gttggcgtca agtttggctc
    30421 gactttgcgg cataagttcc actaattttc catggattgc aaccgctgta gcccgcgcta
    30481 tctctgggga aagccaaaga tgcatgttga aatcgccgtg atggtgatct tcgtcacttt
    30541 tttccgcgtg atcgtgatca tcatcatcgt cgtgaatact tttcatcagc agcggtttca
    30601 cattctctag ctgcgcaatc gttacctgtt tcgcttctgg taatttactt accggttttt
    30661 gcataaacgc ttccatctcc gggccaaccc aaacgactaa gtccgcgttc tgtaagcgtt
    30721 ttacatccga tggacgcagt gaataatcat gttctgaagc gccgtcaggc agtaaaacct
    30781 gcgtttctgt taccccatca gcaatggcag aagcgatgaa cccaacgggt ttaagcgaag
    30841 cgacaacggc ggcatctgcg gcctgtgttg caccgcccca gagagcggcg gataatgctg
    30901 cgaaaagaag cgttttttta tgtaacataa tgcgaccaat catcgtaatg aatatgagaa
    30961 gtgtgatatt ataacatttc atgactactg caagactaaa attaacatga caagtctggt
    31021 ttccctggaa aatgtctcgg tttcttttgg ccaacgccgc gtcctctctg atgtgtcgct
    31081 ggaacttaaa cctggaaaaa ttttgacttt acttgggcca aacggcgcag gtaagtcgac
    31141 actggtacgg gtagtgctcg gactggtaac acccgatgaa ggggttatca agcgcaacgg
    31201 aaaactgcgc atcggctatg taccgcagaa gctgtatctc gacaccacgt tgccactgac
    31261 cgtaaaccgt tttttacgct tacgccctgg cacacataaa gaagatattt tgcctgcact
    31321 gaaacgtgtc caggccgggc atctgattaa cgcaccgatg caaaagctct cgggtggcga
    31381 aacgcagcgt gtactgttag cgcgagcatt gttaaatcga ccgcaattat tagtgctgga
    31441 tgaacccact cagggcgtgg atgtgaatgg tcaggtggcg ttatatgacc ttattgacca
    31501 actgcgtcgt gaactggatt gtggcgtttt aatggtatct cacgatctgc atctggtaat
    31561 ggcaaaaacc gatgaagtgc tttgcctgaa tcaccacatt tgttgttccg gcacaccgga
    31621 agttgtttcc ctgcatccgg agtttatttc tatgtttggt cctcgtggtg ctgaacaact
    31681 gggtatctat cgccatcatc ataatcatcg tcacgattta cagggacgaa ttgttttgcg
    31741 tcggggaaat gatcgctcat gattgaatta ttatttcccg gttggttagc cgggatcatg
    31801 ctcgcctgtg ccgcgggtcc gctgggttcg tttgtagtct ggcgtcgtat gtcttatttc
    31861 ggtgatacgc tggctcatgc ctcattactt ggcgtcgcgt ttggtttgtt gctggacgtg
    31921 aatccattct atgcggtgat tgcagttacg ctgctgctgg cgggcggtct ggtatggctg
    31981 gagaagcgtc cacagctggc gatcgacacg ttattaggga ttatggcgca cagtgccctg
    32041 tcgctgggcc tggtggtcgt tagtctgatg tctaatattc gtgttgattt gatggcttac
    32101 ctgttcggtg atttactggc agtgacgcca gaagatctca tctctattgc gattggcgtg
    32161 gtcatcgtgg tggctatttt gttctggcaa tggcgcaatt tgctgtcgat gacgattagc
    32221 ccggatctgg cgtttgttga tggtgtgaaa ttacagcgcg tgaaattgtt gttgatgctg
    32281 gtgacggcat tgacgattgg tgtagcgatg aaattcgtcg gcgcgttgat tattacttca
    32341 ctgctgatta ttcctgctgc tactgcacgt cgctttgccc gcacgccgga acagatggct
    32401 ggtgtcgctg ttttggtggg gatgctggca gtgactggcg gtttaacctt ttccgcattt
    32461 tacgatacac ctgcaggccc gtcggtggtg ctatgcgcgg cactgttatt tattctcagt
    32521 atgatgaaaa aacaggccag ctaatctttc gctgaacata tttgtcggat gcggcgcgag
    32581 cgccttatcc gacctacggt tcgctatctc tggtaggcct gataagacga acagcgtcgc
    32641 atcaggcaca ctgccagtgt cggatgcggc gcgagcgacc aatccgactt acggcatttc
    32701 tggcggcgtt atgccaaagt gattccacgc ccgcgtcgtc gccatacgcc cacgcggtgt
    32761 acgctgcaaa aagccttgct gaatcaaata aggttccagt acatcctcaa tggtttcacg
    32821 ttcttcgcca atggctgccg ccaggttatc cagacctacc ggcccaccaa agaacttatc
    32881 gattaccgcc agcaacaatt tgcggtccat ataatcgaaa ccttcagcat cgacattcaa
    32941 catatccagc gcctgagcag cgatatctgc cgagatggtg ccatcgtgct tcacttcagc
    33001 gaaatcacgc actcgacgca gcagacggtt ggcaatacgt ggcgtaccgc gcgcacgacg
    33061 agcaacttcc agcgcgccgt catcactcat ctcaagcccc ataaagcgtg cgctgcgact
    33121 gacgatatat tgcagatccg gcacctgata aaactccaga cgttgcacaa taccaaaacg
    33181 atcgcgcaac ggtgatgtca gcgaacctgc gcgcgtggtt gcaccaatca gggtaaacgg
    33241 cggcaaatca attttaatgg agcgtgccgc cggaccttca ccaatcatga tatccagttg
    33301 gtaatcttcc attgccggat acaacacctc ttccaccact ggtgaaagac ggtggatctc
    33361 atcaataaac agtacatcgt gtggttcaag gttagtgagc attgctgcca gatcgcccgc
    33421 cttttccagc accggaccag aagtcgtgcg taaattaacg cccatttcat tggcgacaat
    33481 attggcaagc gtagttttac ccaaccccgg aggaccaaaa atcaatagat gatcgagggc
    33541 atcgccgcgc agtttcgctg ctttgatgaa aatctccatc tgcgaacgaa cctgcggctg
    33601 accaacatac tcttccagta atttagggcg aatggcgcga tctgccacat cttccggcaa
    33661 agtggtaccg gcagaaatca gacggtctgc ttcaatcatc ctttacctca taacgcggcg
    33721 cgtagggctt cgcgaattaa tgtttcactg ctggcgtcag ggcgagcgat tttgctcacc
    33781 atacggcttg cttcttgtgg tttatagccc agcgccacca gcgcagcaac cgcttcctgt
    33841 tcagcatcgt cggtcgccgg gctggcaggt gacgtgagta ccaggtcggc ggctggcgta
    33901 aagagatcgc catgcaaacc tttaaatcgg tctttcattt cgacaatcaa gcgttcggcg
    33961 gtttttttgc caatacccgg cagtttcacc agtgccccca cttcttcacg ctcaacggca
    34021 ttaacgaact gctgcgctga cattccggag aggatcgcca gcgccaactt cgggccgacg
    34081 ccgttggttt tgatcaactc tttgaacaac gtgcgctctt gtttattgtt aaaaccgtac
    34141 agcagttgcg cgtcttcacg caccacaaag tgggtgaaaa cgatcgcttc ctgacccgct
    34201 tcagggagtt cataaaaaca ggtcatcggc atatgcactt catagcctac gccgcccact
    34261 tcaattaaca ccagcggggg ttgtttttca atgatgatgc ctctgagtct gcctatcaca
    34321 tgacgctcct gcgtaatgaa tcaaagataa tgctgtatga taaaaaaatg ctggatagat
    34381 atccagcgaa ggatgaagaa aacttgcgag gtgtcccgat gatctgaaaa atg
//
LOCUS       DAPHCV010000050        33905 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.050, whole genome shotgun sequence.
ACCESSION   DAPHCV010000050 DAPHCV010000000
VERSION     DAPHCV010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.050"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            493..1206
                     /gene="aphA"
                     /locus_tag="RH033_002336"
     CDS             493..1206
                     /gene="aphA"
                     /locus_tag="RH033_002336"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HDV2991512.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKNFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            1317..1733
                     /locus_tag="RH033_002337"
     CDS             1317..1733
                     /locus_tag="RH033_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HDV2991513.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            1737..2093
                     /locus_tag="RH033_002338"
     CDS             1737..2093
                     /locus_tag="RH033_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDV2991514.1"
                     /translation="MTISELLQYCMAKAGAEQSVHSDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(2128..4950)
                     /gene="uvrA"
                     /locus_tag="RH033_002339"
     CDS             complement(2128..4950)
                     /gene="uvrA"
                     /locus_tag="RH033_002339"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDV2991515.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            5205..5732
                     /gene="ssb1"
                     /locus_tag="RH033_002340"
     CDS             5205..5732
                     /gene="ssb1"
                     /locus_tag="RH033_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HDV2991516.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQGGNQFSGGAQSRPQQSAPAA
                     PSNEPPMDFDDDIPF"
     gene            complement(5831..6112)
                     /locus_tag="RH033_002341"
     CDS             complement(5831..6112)
                     /locus_tag="RH033_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HDV2991517.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            6541..8127
                     /gene="pdeC"
                     /locus_tag="RH033_002342"
     CDS             6541..8127
                     /gene="pdeC"
                     /locus_tag="RH033_002342"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HDV2991518.1"
                     /translation="MSHHARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNSQLQT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSNEASAATFSPLIHLNDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLIAHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGVYLATHVDRYVSINLSASDFHTSRLIARTNQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            complement(8130..8453)
                     /gene="soxS"
                     /locus_tag="RH033_002343"
     CDS             complement(8130..8453)
                     /gene="soxS"
                     /locus_tag="RH033_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HDV2991519.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            8539..9003
                     /gene="soxR"
                     /locus_tag="RH033_002344"
     CDS             8539..9003
                     /gene="soxR"
                     /locus_tag="RH033_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HDV2991520.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLISSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIREAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            9549..10898
                     /gene="ghxP"
                     /locus_tag="RH033_002345"
                     /gene_synonym="yjcD"
     CDS             9549..10898
                     /gene="ghxP"
                     /locus_tag="RH033_002345"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HDV2991521.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISSTGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFTTFPVMMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            11049..12698
                     /locus_tag="RH033_002346"
     CDS             11049..12698
                     /locus_tag="RH033_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HDV2991522.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(12781..14430)
                     /gene="actP"
                     /locus_tag="RH033_002347"
     CDS             complement(12781..14430)
                     /gene="actP"
                     /locus_tag="RH033_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HDV2991523.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(14427..14741)
                     /locus_tag="RH033_002348"
     CDS             complement(14427..14741)
                     /locus_tag="RH033_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HDV2991524.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(15032..16990)
                     /gene="acs"
                     /locus_tag="RH033_002349"
     CDS             complement(15032..16990)
                     /gene="acs"
                     /locus_tag="RH033_002349"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HDV2991525.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSIEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGHPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            17382..18818
                     /gene="nrfA"
                     /locus_tag="RH033_002350"
     CDS             17382..18818
                     /gene="nrfA"
                     /locus_tag="RH033_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HDV2991526.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPATPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            18863..19429
                     /gene="nrfB"
                     /locus_tag="RH033_002351"
     CDS             18863..19429
                     /gene="nrfB"
                     /locus_tag="RH033_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HDV2991527.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            19426..20097
                     /gene="nrfC"
                     /locus_tag="RH033_002352"
     CDS             19426..20097
                     /gene="nrfC"
                     /locus_tag="RH033_002352"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HDV2991528.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEVSQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            20094..21050
                     /gene="nrfD"
                     /locus_tag="RH033_002353"
     CDS             20094..21050
                     /gene="nrfD"
                     /locus_tag="RH033_002353"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HDV2991529.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     SMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            21070..22788
                     /locus_tag="RH033_002354"
     CDS             21070..22788
                     /locus_tag="RH033_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HDV2991530.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGTCARICWRWARPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFVLISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGPDRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            22781..23164
                     /gene="nrfF"
                     /locus_tag="RH033_002355"
     CDS             22781..23164
                     /gene="nrfF"
                     /locus_tag="RH033_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HDV2991531.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQEQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYTMVEEGKSEVEIIGWMTERYGDFVRYNPPLTGQT
                     LLLWALPVVLLLLMALILWRVRAKR"
     gene            23161..23757
                     /gene="nrfG"
                     /locus_tag="RH033_002356"
     CDS             23161..23757
                     /gene="nrfG"
                     /locus_tag="RH033_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HDV2991532.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVSSYLLSPKWQAVHAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            24099..25412
                     /gene="gltP"
                     /locus_tag="RH033_002357"
     CDS             24099..25412
                     /gene="gltP"
                     /locus_tag="RH033_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HDV2991533.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(25830..26519)
                     /locus_tag="RH033_002358"
     CDS             complement(25830..26519)
                     /locus_tag="RH033_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDV2991534.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(26613..28760)
                     /gene="fdhF"
                     /locus_tag="RH033_002359"
     CDS             complement(26613..28760)
                     /gene="fdhF"
                     /locus_tag="RH033_002359"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(28341..28343),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HDV2991535.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDTDQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(28958..30424)
                     /gene="mdtP"
                     /locus_tag="RH033_002360"
     CDS             complement(28958..30424)
                     /gene="mdtP"
                     /locus_tag="RH033_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HDV2991536.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLLEEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRTASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(30421..32472)
                     /gene="mdtO"
                     /locus_tag="RH033_002361"
     CDS             complement(30421..32472)
                     /gene="mdtO"
                     /locus_tag="RH033_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HDV2991537.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWLSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAIAEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     TKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMALRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTFLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQVQGAT
                     Q"
     gene            complement(32472..33503)
                     /gene="mdtN"
                     /locus_tag="RH033_002362"
     CDS             complement(32472..33503)
                     /gene="mdtN"
                     /locus_tag="RH033_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HDV2991538.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQASDTLRRTEPLLREGFVSAEEVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(33522..33797)
                     /locus_tag="RH033_002363"
     CDS             complement(33522..33797)
                     /locus_tag="RH033_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2991539.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPGWF
                     FCAIASLILTLITRRIIQRTNINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         7700 a         8741 c         9145 g         8319 t
ORIGIN      
        1 ctttgtcata agagatacct ttgttattta gtgtatctag ctctacaggt aaagatgtcg
       61 tgttgttgct attcacattt gatatattca tatcatcatc tccttcggga gtaataaaat
      121 tagtcaccat tgtagggtag ggggctggtc aatcagaaat catctttata aacttcgatt
      181 gtttttgtaa tgctgtatca ttaagtttat taaatcgtac agcagataaa tgttctatca
      241 aatttcgctc atttgcagag gattcatcat aataaacgca aaattaatgt attcttacat
      301 cgcgtaataa acattttttt tacaaaaaac gacaggaacg tatttactgg gttaaatata
      361 atcatcctgc ttttcatcac aaaaaccgca gataatcctt cctttcccct gctgctggcg
      421 ttatggtcag atggtttttg caacaaatct cacaataaaa agtttcaaca tactgactat
      481 ttagggaaaa atatgcgcaa gatcacacag gcaatcagtg ccgtttgctt attgttcgct
      541 ctaaacagtt ccgctgttgc cctggcctca tctccttcac cgcttaaccc tggtactaac
      601 gttgccaggc ttgctgaaca ggcaccaatt cattgggttt cggtcgcaca aattgaaaat
      661 agcctcgcag ggcgtccgcc aatggcggtg gggtttgata tcgatgacac ggtacttttt
      721 tccagtccgg gcttctggcg cggcaaaaaa aacttctcgc cagaaagcga agattatctg
      781 aaaaatcctg tgttctggga aaaaatgaac aatggctggg atgaatttag cattccaaaa
      841 gaggtcgctc gccagctgat tgatatgcat gtacgccgcg gtgacgcgat cttctttgtg
      901 actggtcgta gcccgacgaa aacagaaacg gtttcaaaaa cgctggcgga taattttcat
      961 attcctgcca ccaacatgaa tccggtgatc tttgcgggtg ataaaccagg gcaaaataca
     1021 aaatcgcaat ggctgcagga taaaaatatc cgaatttttt atggcgattc cgataacgat
     1081 attaccgccg cacgcgatgt cggcgctcgt ggtatccgca ttctgcgcgc ctccaactct
     1141 acctacaaac ccttgccaca agcgggtgcg tttggtgaag aggtgatcgt caattcagaa
     1201 tactgacaga gcgggagagc gtgacactct cccgaatgct gtttttttaa tcacaccttt
     1261 atcctttcgc tgtcttgctg caaactgatt aagagagttt tatcaaggag cagcacatgt
     1321 ggtatcaaaa gacgctcacg cttagcgcca aatctcgtgg gtttcatctg gtaacggatg
     1381 aaattctgaa tcagcttgct gatatgccgc gcgttaacat cggtttactg catctgttgc
     1441 tgcaacatac ctccgcctct ctgacactta atgagaactg cgatcccacc gtacgccacg
     1501 acatggagcg ttttttcctc cgcaccgttc ccgacaacgg aaattatgag catgactatg
     1561 agggagcaga cgatatgcct tctcatatca aatcctcaat gctgggaaca tcgcttgtat
     1621 tgccggtgca taaagggcgt attcagaccg gcacctggca aggcatctgg ctgggggaac
     1681 atcgcatcca cggcggatcg cgtcgcatta tcgcgacact acaaggggag taaaaaatga
     1741 ccatttcgga gttgctacaa tattgcatgg ctaaagctgg cgcggaacag agcgttcaca
     1801 gcgactggaa agcgacgcag atcaaagttg aagatgtgct ttttgcgatg gtgaaagagg
     1861 ttgaaaatcg cccagctgtt tcgctgaaaa ccagcccgga gctggcagag ttgttacgtc
     1921 agcagcacag tgatgtgcgt ccgagccgcc atctgaacaa agcgcactgg agcactgttt
     1981 atctcgacgg ttcgctgccg gattcgcaaa tctattatct ggtggatgcg tcttatcagc
     2041 aggcggtgaa tttgctgccg gaagaaaaac gtaaattgct ggtgcaactc tgaaaggaaa
     2101 aggccgctca gaaagcggcc ttaacgatta cagcatcggc ttgaggaagc gcgccgtatg
     2161 cgaagcttcg cactccgcga cggtttctgg cgtaccggag acgaggattt cgccgccgcc
     2221 acttccgcct tccggtccca ggtcgacaat ccagtcagcg gttttgatca cgtcgagatt
     2281 gtgctcaatc accacaatgg tgttgccctg atcgcgcagt ttatgcagta cgtcgagcag
     2341 ttgctgaata tcggcgaagt gcagaccggt ggtcggctca tcaagaatat acagcgtctg
     2401 cccggtgccg cgttttgaca gctcacgcgc cagcttcacg cgctgggctt caccaccaga
     2461 aagcgtggtt gcggactgcc cgaggcgaat gtacgtcagg ccaacgtcca tcaacgtttg
     2521 cagcttacgc gccagagctg gcaccgcatc aaagaactca cgcgcctctt cgatggtcat
     2581 atccagcact tcgtggatgg ttttgccttt gtacttaatc tccagcgttt cacggttata
     2641 gcgtttacct ttgcactggt cgcacggtac gtaaatgtcc ggcaggaagt gcatctccac
     2701 tttgatcacg ccgtcgccct gacaggcttc gcagcgcccg ccacggacgt taaagctgaa
     2761 acgtcccggc gtatagccgc gcgcacggga ttccggtacg cccgcaaaca gttcgcgcac
     2821 aggcgtaaac acgccggtat aggtcgccgg gttagaacgc ggagtacgac caattgggct
     2881 ttggtcgata tcgatcactt tatcgaaatg ctccagcccc tgaatatcgc gatacggtgc
     2941 cggttcggcg atggtcgcac cattcaactg gcgttgggca atcgggaaca gtgtgtcgtt
     3001 aatcagcgtc gatttaccgg aacctgaaac ccctgtgatg caggtaaaca gaccgactgg
     3061 cagcgtcagc gtcacgtctt tcaggttatt accacgtgcg cctgtcagct tcagcacttt
     3121 ttccggattc gccggaacgc gtttcttcgg cacttcaatc ttgcgtttac cgctcatgta
     3181 ctgcccggtc aacgactcag gcactgccat aatcgcttcc agtggacctt ctgcgaccac
     3241 ttcaccgccg tgtacacctg cgcccgggcc aatgtcgatc acatggtcag cggcgcgaat
     3301 cgcgtcttcg tcgtgctcca ccacaatcac ggtattaccg agatcgcgca gatggataag
     3361 cgtacccaac aggcgctcgt tatcgcgctg gtgcaggccg atagacggct cgtccagcac
     3421 atacataacg ccaaccaggc ccgcaccaat ctggctcgcc agacggatac gctgtgcttc
     3481 accgccagaa agcgtttctg ccgagcggga aagtgtcagg taattcaggc ctacgttaac
     3541 gaggaatttc aggcgatcgc caatctcttt aagaattttt tccgcaatct tcgctcgctg
     3601 acctgcgagt ttgagattgt tgaagaattc catcgcatga ccgatgctca tgtcggagat
     3661 ggcgggcagc ggcgtattct cgacatacac gtggcgtgct tcccgacgca gacgggttcc
     3721 ttcgcagctg gcgcacggac gattgctgat aaacttggct aattcttcac gtaccgcact
     3781 ggattccgtc tctttataac ggcgctccat attgtgcagc acgccttcga acggatgacg
     3841 acgaatggag gtatcgccac gatcgttcat gtatttgaat tcaatgtttt ctttgccaga
     3901 accgtacaac accactttat gcacgttcgc gctcaggctg ccccacggcg cttcgacgtc
     3961 gaacttatag tgatctgcca gcgatttcag catctggaag taatagaagt tgcggcgatc
     4021 ccagcctcgg atcgcaccac ctgccagcga cagttccgga ttctggatca cgcggtcagg
     4081 atcgaaatat tgctgtacgc caaggccgtc acaggtcggg caggcacccg ccgggttgtt
     4141 aaacgaaaac aggcgtggtt ccagttcacg catactgtag ccgcaaattg ggcaggcgaa
     4201 gttggcggaa aatagcagct cttccgcttt tggatcgtcc atatcggcaa ctaccgcggt
     4261 accaccggaa agctccagcg cggtttcaaa cgactctgcc agacgttggg tgagatcgtc
     4321 acgcaccttg aagcgatcaa ccaccacttc aatggtatgt ttcttttgca gttccagttt
     4381 cggcggatcg gaaagatcgc agacttcgcc atcaatacga gcacggatgt aaccctggct
     4441 tgccagattt tccagcgttt tggtgtgttc gcctttgcgc tctttaatga ttggcgcgag
     4501 cagcatcagg cgtttacctt ccggctggga caacacgtta tccaccatct gactgacggt
     4561 ttgcgccgcc agcggtacat cgtggtccgg gcagcgcggc tcaccgacgc gggcgaacaa
     4621 caaacgcaaa tagtcgtgga tttcggtgat tgtccccacc gtagaacgcg ggttatgaga
     4681 cgtcgatttc tgctcaatcg agatggcagg agaaagcccc tcaatatgat cgacgtccgg
     4741 cttttccatc agtgacagaa actgccgcgc gtaggcggaa agggattcaa cgtaacggcg
     4801 ctgcccttcg gcatataagg tgtcgaaagc gagcgaggat ttgccagaac ccgaaagccc
     4861 ggtcacgaca atgagtttgt cgcgggggat aacgaggttg atgtttttga gattatgggt
     4921 gcgggcgccc cgaacttcga tcttatccat tcacctttcc cggattaaac gcttttttgc
     4981 ccggtggcat ggtgctaccg gcgatcacaa acggttaatt atgacacaaa ttgacctgaa
     5041 tgaatataca gtattggaat gcattacccg gagtgttgtg taacaatgtc tggccaggtt
     5101 tgtttcccgg aaccgaggtc acaacatagt aaaagcgcta ttggtaatgg tacaatcgcg
     5161 cgtttacact tattcagaac gatttttttt caggagacac gaacatggcc agcagaggcg
     5221 taaacaaggt tattctcgtt ggtaatctgg gtcaggaccc ggaagtacgc tacatgccaa
     5281 atggtggcgc agttgccaac attacgctgg ctacttccga atcctggcgt gataaagcga
     5341 ccggcgagat gaaagagcag actgaatggc accgcgttgt gctgttcggc aaactggcag
     5401 aagtggccag cgaatatctg cgtaaaggtt ctcaggttta tatcgaaggt cagctgcgta
     5461 cccgcaaatg gaccgatcaa tccggtcagg atcgctacac cacagaagtc gtggtgaacg
     5521 ttggcggcac catgcagatg ttgggtggtc gtcagggtgg tggcgctcct gcaggtggca
     5581 atatcggtgg tggtcagccg cagggcggtt ggggtcagcc tcagggcggc aatcagttca
     5641 gcggcggcgc gcagtctcgc ccgcagcagt ctgctccggc agcaccgtct aatgagccgc
     5701 cgatggactt tgatgacgat attccgttct gatttttcat taaaacaata ggttatattg
     5761 ttttaaggtg gatgattaaa acatctgcca gccgtaaaaa agaagcctcc gttttggagg
     5821 cttctacata tcaagtcaaa atcattggcc attgtggcgg tgtctgattc atctcttcta
     5881 tcatcaccga ctcgatgttg tgccagatgg cggtgatatc cgccattgtg atgccaagca
     5941 accccattgc gaaccagcag gcagaaacca cgcccagacc gctgctgatc actgcaagcc
     6001 cgacataatc agaccgacga aaacggatgt tgagcgtgct ggccagaaac atcattacgg
     6061 cactaagaag ttgccagcga agaagaacca cgccagtggt gagggtagcc atcaaacaat
     6121 tcctctgaaa agagccgatg ccctggacag cgcggcgttg ttcacgggag gcgtggtaca
     6181 ctctggctat cgcggggctt gcaggacaca aaaatgaaac actctgtttg tttcattaat
     6241 tttgtgaact atatcacaat tgattgtttg ttagccagat taggccgtga cttttattgc
     6301 tgtacagatt atgtggtttt tcagcggcat taagggcata tcttcccgcc gcctctgcat
     6361 tcctgtagaa aattaatttg aatatcaatg aattattttt atccaggtga ccattaaaaa
     6421 ggtatcaatt tcaaatcagg caaaagtgct atttataccg taagatttat ctaaagacgt
     6481 cggtaaccag ggttttcacc ttgcaatgac cgggtataaa caggcaggaa attgatagca
     6541 atgagtcatc atgcacgaca ccaattactg gcgttgccgg gcattatctt tttagttctc
     6601 tttcccatca ttctgtcgct atggattgcc ttcctttggg caaaatcaga agtgaatagc
     6661 cagctccaaa cttttgctca gctggcgctg gataaatccg agctggtcat tcgccaggca
     6721 gatttagtga gcgatgcagc agaacgctat caggggcaag tttgcactcc agcccatcaa
     6781 aagcgaatgt tgaatattat tcgtggctat ctttatatta atgaattgat ctatgcccgt
     6841 gataaccatt ttttatgctc atcgctgata gcgcctgtaa acggctatac gattgcaccg
     6901 gccgattata agcgtgaacc taacgtttct atctattatt accgcgatac gccttttttc
     6961 tctggctata aaatgaccta tatgcagcgg ggaaattatg tggcggttat caaccctctc
     7021 ttctggagtg aagtgatgtc tgatgatccg acattgcaat ggggtgtgta tgatacggtg
     7081 acaaaaacct ttttctcgtt aagcaacgag gcctcggcag caacgttttc tccgctgatt
     7141 catttgaatg atttaaccgt acaaagaaat ggctatttat atgcgacagt ttattcgaca
     7201 aaacgcccga ttgcagccat tgttgcgact tcatatcaac gtcttatagc gcatttttat
     7261 aatcatctta tttttgcgtt acccgccggt attttgggga gtcttgttct gttattactc
     7321 tggctacgta ttcgacaaaa ctatttgtct cccaaacgca aattgcaacg cgccctcgaa
     7381 aaacatcaac tttgccttta ttaccagcca ataatcgata tcaaaacaga aaaatgtatc
     7441 ggagctgaag cattgttacg ttggcctggt gagcaggggc aagtaatgaa tccggcagag
     7501 tttattccgc tggcagaaaa ggaggggatg atcgaacaga taactgatta tgttattgat
     7561 aatgtcttcc gcgatctggg cgtgtacctg gcaacacatg tcgatcgcta tgtttctatt
     7621 aacctgtcgg cctccgattt tcatacgtca cggttgatag cgcgaaccaa ccagaaaaca
     7681 gagcaatatg cggtgcgtcc gcagcaaatt aaatttgaag tgactgaaca tgcatttctt
     7741 gatgtcgaca aaatgacgcc gattattctg gctttccgcc aggctggtta cgaagtggca
     7801 attgatgatt ttggtattgg ctactctaac ttgcataacc ttaaatcatt gaatgtcgat
     7861 attttgaaaa tcgataaatc gtttgttgag acgctgacca cccataaaac cagtcatttg
     7921 attgcggaac acatcatcga gctggcgcac agtctggggt taaaaacgat cgctgaaggc
     7981 gtcgaaactg aggagcaggt taactggctg cgcaaacgcg gcgtgcgcta ttgccaggga
     8041 tggttctttg cgaaggcgat gccgccgcag gtgtttatgc aatggatgga gcaattaccc
     8101 gcgcgggagt taacgcgcgg gcaataaaac tacaggcggt ggcgataatc gctgggagtg
     8161 cgatcaaact gccgacggaa aacgcgggag aaggtctgct gcgagacata acccaggtcc
     8221 attgcgatat caaaaatcgg acgctcggtg gtgcgcaact caacggcggc cagtaacagg
     8281 cggcgttggc gaatgtaatc acctagtgtc tgatgcgtca cagtacggaa catccgctgt
     8341 aagtaccact ttgaatagcc tgattttttt gcgacgacat caatgttaag cggctggtca
     8401 atatgctcgt caatccatgc gataagatcc tgaataattt tctgatggga cataaatctg
     8461 cctcttttca gtgttcagtt cgttaattca tctgttgggg agtataattc ctcaagttaa
     8521 cttgaggtaa agcgatttat ggaaaagaaa ttaccccgca ttaaagcgct gctaaccccc
     8581 ggcgaagtgg caaaacgcag cggtgtggcg gtatcggcgc tgcatttcta tgaaagcaaa
     8641 gggttgatta gcagtatccg taatagtggc aatcagcggc gatataaacg tgatgtgttg
     8701 cgatatgtcg caatcatcaa aattgctcag cgtattggca ttccgctggc gaccattcgt
     8761 gaagcatttg gcgtgttgcc cgaagggcat acattaagtg cgaaagagtg gaaacagctt
     8821 tcgtcccaat ggcgagaaga gttggatcgg cgcattcata ccttagtggc gctgcgtgac
     8881 gagctggacg gatgtattgg ttgtggctgc ctttcgcgca gtgattgccc attgcgtaac
     8941 ccgggcgacc gcttaggaga agaaggcaca ggcgcacgct tgctggaaga tgaacaaaac
     9001 taaagcgcca caagggcgct ttagtttgtt ttccggtctt tgtctttctc tctatcccgc
     9061 tggtacacag gagggtttcc cccgacgtca acacacctca ttcgagcacg tggtggaggt
     9121 tccggttggt gttgatgctt taattgtatg tcaccggcgt ttcttcgcca gtgtaaaagt
     9181 acacttttta gccgcaatat ttttgtcatc tcagacgatt ttttatcgca atcctgaacg
     9241 gtatacggct cgataacgct gcaatcttgc gcatcgacga taacgtttgc gcatcaattg
     9301 cctggttttt catcgtcaag acaataaaag agaaaaaagc agcaaacttc ggttgaaaaa
     9361 gccgctatga tcgccggata atcgtttgct ttttttacca cccgttttgt atgcgcggag
     9421 ctaaacgttt gcttttttgc gacgcaccaa tttgtcgcaa acctggagca ggaagataac
     9481 gtttcgctgg caggggattg tccgccacgc atcttgacga aaattaaact ctcaggggat
     9541 gttttcttat gtctacgcca tcagcgcgta ccggcggttc actcgacgcc tggtttaaaa
     9601 tttcacaacg tggaagcact gtccgtcagg aagtggttgc cgggttaaca acatttctgg
     9661 cgatggtcta ctcggttatc gtcgttccgg gcatgttggg taaagcaggc ttcccgcctg
     9721 cggcagtttt cgttgcgacc tgtctggttg ccggactcgg ttctatcgtg atgggtctgt
     9781 gggctaatct gccgttggcg attggttgcg ccatctccct gacggcgttt accgcattca
     9841 gtctggtgct ggggcaacat attagcgtac ctgtcgcgct gggtgccgtg ttcctgatgg
     9901 gtgtgctgtt tacggtcatt tcttccacgg gtatccgtag ctggattttg cgcaacttgc
     9961 cgcacggtgt ggcgcacggc acggggattg gtatcggtct gttcctgctg ctgattgctg
    10021 ctaatggtgt cggtctggtg attaaaaacc cacttgatgg tctgcccgtc gcgctggggg
    10081 atttcacgac ctttccggtg atgatgtcgt tggtggggct ggcagtgatt atcggcctgg
    10141 aaaaactgaa agtccctggt ggcattctgc tgaccattat cggtatctca attgtcggtt
    10201 tgatcttcga tcctaacgtc catttctccg gcgttttcgc catgccttca ttgagcgatg
    10261 aaaacggcaa ttcactaatt ggcagcctgg acattatggg cgcgctgaat cctgtagtcc
    10321 tgccaagcgt tctggcgctg gtgatgacgg cagtatttga tgccaccgga actatccgtg
    10381 ccgtcgccgg ccaggcgaac ctgctggata aagatgggca gatcatcgac ggcgggaaag
    10441 cactgaccac tgactccatg agcagcgttt tctctggcct ggtgggtgca gctccggcag
    10501 cggtatacat cgagtctgcg gcgggtacgg cggcgggcgg taaaaccggt ttgacggcta
    10561 tcaccgttgg cgtgctgttc ctgctgattc tgttcctctc tccgctctct tacctcgttc
    10621 cggggtatgc aacggctccg gcgctgatgt acgttggcct gctgatgctg agtaacgtgg
    10681 cgaaaatcga ctttgctgat tttgttgatg cgatggcggg tctggttacg gcggtgttca
    10741 tcgtgctgac ctgtaacatc gtaacaggca tcatgatcgg cttcgcgact ctggtgattg
    10801 gtcgtctggt ttccggcgaa tggcgcaagt tgaacatcgg tacggtcgtt atcgctgtgg
    10861 cgctggtgac cttctatgcg ggtggctggg ctatctaatc tttcatatga ttctgaaaac
    10921 gggtggcaat ggctgcccgt ttttattttc tccgcacatt gtgtgagctt ttgcgatatt
    10981 ctgaaaaaat gagaattcag gcataacgcc tttctcagat cataaaaaac atcgcaaaca
    11041 gggaacgcat ggaaatcttc ttcaccatac tgataatgac cctcgtggtc tcgctgtccg
    11101 gggtggtcac tcgtgtcatg ccctttcaga tcccgcttcc gcttatgcaa atcgccatcg
    11161 gtgcgctact ggcgtggccg acgtttggtt tgcatgtgga gtttgatcct gaactctttt
    11221 tagtcttgtt tatcccgccg ttgctgttcg ctgatggctg gaaaacgccg acccgtgaat
    11281 ttctcgaaca tggtcgagag attttcggcc tcgcgctggc gctggtggtg gtcaccgtgg
    11341 tcggcattgg ctttcttatt tactgggtgg tgccgggcat tccgctgatc cccgcctttg
    11401 cgctggcggc ggtgctttct ccgaccgatg ctgtggcgct ctcggggatt gttggcgaag
    11461 ggcgcatccc gaaaaaaatc atgggcattt tgcagggcga agcgttgatg aatgacgcct
    11521 ccggtctggt gtcgttgaag tttgccgtgg cagtggcgat ggggacgatg atcttcaccg
    11581 tcggtggtgc gacggtcgaa tttatgaaag tggccattgg cggtattctc gccggttttg
    11641 tggtgagctg gctgtacggt cgctcgctgc gattcctcag ccgctggggc ggtgatgaac
    11701 ccgcgacgca gatcgtcctg ctgttcctgc tgccattcgc ttcttatctg attgccgaac
    11761 atattggcgt ttccggtatc ctcgctgcgg ttgccgccgg gatgaccatc acccgttccg
    11821 gtgtgatgcg ccgtgcgccg ctggcaatgc gcctgcgcgc aaacagtacc tgggcgatgc
    11881 tggaatttgt ctttaacggc atggtattcc tgctgttagg tctgcagctg ccgggtattc
    11941 tggagacgtc gctgatggcg gcagaaatcg accctaacgt cgaaatctgg atgctgttta
    12001 ccgatatcat tctgatttat gcggcgctga tgctggtccg tttcggctgg ctgtggacga
    12061 tgaaaaagtt cagcaaccgc ttcctgaaga agaagccgat ggagtttggt tcgtggacca
    12121 cacgagaaat cctgatcgcg tctttcgccg gggtgcgtgg ggcgatcact ctggctggtg
    12181 tgctctcaat cccgctgctc ttgccggatg gtaacgtctt cccggcgcgc tatgagctgg
    12241 tgttcctggc ggctggtgtc attctcttct cgctgtttgt cggcgtggtg atgttgccta
    12301 ttctgctaca acacattgaa gtcgcggacc attcgcaaca attgaaagag gaacgtattg
    12361 cgcgagcggc aacggcagaa gtggcgattg tggcgatcca gaaaatggag gagcgtctgg
    12421 cggcggatac cgaagagaat atcgataacc agctgctcac agaggttagt tctcgcgtca
    12481 ttggtaacct gcgtcgtcgc gccgatggac gtaacgacgt tgaaagttcc gtgcaggaag
    12541 agaaccttga gcgtcgcttc cgtctggcgg cattgcgttc tgaacgtgct gaactgtacc
    12601 acctgcgcgc cacgcgggag atcagcaatg aaacgctgca aaaattactg cacgatctcg
    12661 atttgcttga agcgttgttg attgaagaga accaataacg ctttggcggt gtaatcggta
    12721 aaaaggtgca gtacatcgca cctttttacc gttggtcagg tgaccagagg ctagtggaga
    12781 ttaatgcgcg cggccttgct caacgccaaa gccagtctgg gagcggataa actgcgcacg
    12841 gaacagttca cgctcgcgcg caccttccgc tgaattatcc gttgccgaga agaaccagat
    12901 accgaggaat gccacgctga tagagaacag tgccgggtat tcatacggga agatggcttt
    12961 ttcgtgaccg aggatctgta cccaaatcgt cggaccgagg atcatcagca ccaccgcagt
    13021 aatcagtcct aaccagccgc ccagcattgc gccacgggta gtcagtttcg accagtacat
    13081 ggaaagcaga atgatcggga agttacagct cgccgcgatg gcaaacgcca gccccaccat
    13141 aaaggcgatg ttctgattct caaacagcac gccgaggata atggcaatca cacccaaaat
    13201 cagaacggtg attttcgata cccgcagctc ttcacgttcg gtcgcgcctt ttttgaagac
    13261 gttagcgtac aggtcgtgag acaccgccga tgcgcccgcc agcgtcagcc ccgctaccac
    13321 cgcgaggata gtggcgaaag caaccgctga aataaagccg aggaacaggt tgccgcccac
    13381 cgcattggcc aggtgaactg ccgccatgtt gttaccacca atcagatggc ccgcagcatc
    13441 tttatattcc ggattcgcac caaccagcat gattgcgccg aagccgataa taaaggtcag
    13501 gatgtagaag tagcccataa atccggtggc gtagaacacg ctcttacgtg cttcgcgggc
    13561 atcgctgacc gtaaagaagc gcatcagaat atgcggcaag cccgccgtac caaacatcag
    13621 tccgagcccc agcgacagtg ctgagatcgg atctttcacc agcccgcctg gcttcatgat
    13681 atcgacacct ttcgggtgta ccgccatcgc ttcgctgaac agattgttga agctaaagcc
    13741 gacgtgtttc atcaccataa aggccataaa gctggcaccg aacagcaaca gaacggcttt
    13801 gataatctgt acccaggtgg tcgccagcat gccgccgaac aggacgtaca tcatcatcag
    13861 cacgccgacc agcaccaccg caatgtgata gttaaggcca aacagcagct cgatgagttt
    13921 acctgcgccc accatctggg cgataaggta aagcgccacc accaccagag aaccacaggc
    13981 cgaaagaata cgaatcggcc cttgtttcag gcggtaagag gctacatcgg caaaggtgta
    14041 gcggcccagg ttacgcagac gttcggcaat caggaacaga atgattggcc agcctaccag
    14101 gaagcccagc gagtaaatta agccgtcata gccggaggta aacaccagcg cggagatccc
    14161 caagaatgag gcggcggaca tatagtcccc ggcaatcgcc agcccattct gaaaaccggt
    14221 gatattgccg cctgcggtgt agtagtcatt acgcgaacgt acgcgttttg acgcccagta
    14281 ggtaatgccg agcgtaaaca cgacgaaaat caggaacata ataatcgcct gccagtttgt
    14341 tggctggcgc tctacggccc cgctaatagc atccgcggcg ttagctgcga aagggagtgt
    14401 ggcggcaagc gccgtcagaa ctcttttcat gatgcttgta cctcatgcag gacttcatta
    14461 ttaagacggt cgaattcgcc gttcgcccgc cagatgtaga taccagtgag aacaaaggag
    14521 atcacaatta ctccaacacc aatcggaata ccgcgtgtga cgctggtgtt cggattcagc
    14581 ggggtgccca gccagccggg cgcgaaggcg atcagtaaaa taaagccgat ataaactgcc
    14641 agcataataa tcgacaggat ggtggcaaac cgttgccgtt tttcgactaa ctccctgaaa
    14701 tgcgcattgt cttctatccg ctgataaatc gtaccattca tcacagattc tccagaggta
    14761 atgtagggat tgttttaatt cccgctccct tatgggagaa ggttaacgct tgggtaaccc
    14821 ttgccgaatg taggccggat aaggcattca cgccgcatcc ggcaatcaac gcctgatgcg
    14881 acgctgtcgc gtcttatcag gcctacagcc gttgccgaat gtaggccgga taaggcgttt
    14941 acgccgcatc cggcaatcaa cgcctgatgc gtcgctgtcg cgtcttatca ggcctacaaa
    15001 ccgttaccga ctcgcatccg gcaattgtgg gttacgatgg catcgcgatg gcctgcttct
    15061 cttcaagcag cttctcgact acgccaggat cggcaagcgt cgaggtatcg cccaggttgc
    15121 tggtatcgcc cgccgcaatt ttgcgcagaa tacggcgcat aattttgccg gagcgggttt
    15181 taggcaggga gtcggtccag tgcagcacgt ctggcgtcgc cagcgggcca atctctttac
    15241 gcacccagtt gcggacttct gcgtacagtt ctggtgacgg ttcctccccg tgattaagcg
    15301 tgacgtaggc gtagatcgcc tgacctttaa tattgtgcgg aatacctact acggcggctt
    15361 cggcaatctt cggatgtgcc accagcgccg actcaatctc tgccgttccc agacggtgac
    15421 cggagacgtt cagcacgtcg tccacgcgcc cggtgatcca gtaatagcca tcttcatcgc
    15481 gacgcgcgcc gtcgccgctg aaatacatat ttttgaaggt ggagaagtag gtctgctcaa
    15541 aacgttcgtg atcgccaaac agcgtacgcg cctgacccgg ccaggagtcg gtgatcacca
    15601 gactgccttc ggtggcccct tccagcgggt gaccttcgtt atcgaccagc gccggttgca
    15661 cgccgaagaa cggacgtgtt gccgaaccgg ctttcagctc ggtagcgcca ggcagtgggg
    15721 tgatcatgaa accgccagtt tcggtctgcc accaggtatc gaccactggg catttctcgt
    15781 tgccgatttt tttccagtac cactcccacg cttccgggtt gattggctcg cccacggaac
    15841 cgagaatgcg cagcgacgaa cggtcagtac cttcgatcgc tttatcacct tccgccatca
    15901 gcgcacgaat cgccgtgggc gcggtataga gaatattgac ctgatgcttg tccaccacct
    15961 gcgccatacg ggcaggcgtc ggccagttcg gtacgccttc aaacatcagc gtggtcgcgc
    16021 cgcaggccag cgggccgtac agcaaataac tgtgtccggt aacccagccc acatcggcgg
    16081 tgcaccagta gatatcaccc ggatgataat caaagacata tttaaaggtc agcgccgcgt
    16141 acaccagata accgccggta gtgtgcagca cgcctttcgg cttcccggta gaaccggagg
    16201 tgtagagaat aaacagcgga tcttcggcgt tcatctcttc cgcctggtgc tgatcgctgg
    16261 cttgctcaac caggtcgtgc caccagaggt cgcgcccttc ctgccagtca attttcccgc
    16321 cagtacgctt cagtaccacc acatgttcaa tgctagtgac gttcgggttt ttcagcgcgt
    16381 catcaacgtt tttcttcagc ggaatactac gcccggcacg cacgccttcg tcggaagtga
    16441 tcaccaaccg tgagttagaa tcgataatgc gtccggcaac ggcttccggc gagaagccgc
    16501 cgaaaattac cgaatgcacc gcgccaatgc gggcgcaggc cagcatcgcg accgcagctt
    16561 ccggtaccat cggcatataa atcgccacca catcaccttt tttaatgccc agctcgagca
    16621 gggtattggc gaagcggcag acgtcgcggt gcagctcttt atagctgata tgtttgctct
    16681 ggctcgcgtc gtcgccttcc cagatgatgg cggtacgatc gccgttttct tgcagatggc
    16741 ggtcaaggca gtttgccgcc agattcagcg tgccgtcctc gtaccattta atggacacat
    16801 taccgggggc aaaggaggtg tttttcacct tctggtaagg tttgatccag tcgagaattt
    16861 ttccctgttc gccccagaag gtatcaggta cgttaataga ttgttgatac atcgcctcgt
    16921 actgctgagg gtttatcagg caacggtctg cgatgttggc aggaatggtg tgtttgtgaa
    16981 tttgactcat gcttttgttc tccttgtaag atgttaataa tatgtggcat aagcgttaaa
    17041 tgtaggggta ttggcagttt tgtttagtat ttgggcgaca gatcacgcaa aagtagaatt
    17101 gtgcaaataa acggcagggt aatttttgaa ggtcagcaac aaaagttgat taattctttg
    17161 aggaacatgc agttatgcat gctgttgaaa aagaggaaga tactgactaa ctctaaagtg
    17221 gtattttaca tgcacttaca attgattaaa gacaacattc atagtgtggt tatttgttac
    17281 acatagggac gagcaatgtc atgacagtgt aggtgcggtt actgtcgtga aaaacaataa
    17341 aaaccgccat tgcaacaatg gcgcaattcg gatgaagccc tatgacaagg ataaaaataa
    17401 acgcacgccg tatcttcagc ttattgattc cttttttctt tttcacttct gttcacgctg
    17461 aacaaacggc tgctcccgca acacctgtaa ctgtggaagc gaagaatgaa acctttgccc
    17521 cgcagcatcc cgatcaatat ctctcctgga aagctacctc ggaacagtca gagcgtgttg
    17581 acgccctggc ggaagatcca cggctggtga tcctgtgggc agggtatccc ttctcgcgcg
    17641 attacaacaa gccgcgtgga catgcctttg ctgtgaccga tgtgcgtgaa accctgcgta
    17701 ccggtgcgcc gaaaaacgct gaagatggtc cgctaccgat ggcatgctgg agttgtaaaa
    17761 gcccggatgt ggcgcgtctg atccagaaag acggcgaaga tggctacttc cacggaaaat
    17821 gggcgcgcgg cggcccggaa atcgtcaaca acttaggttg tgccgattgc cataacaccg
    17881 cctcaccaga gttcgccaaa ggcaaaccgg agttaaccct ttcccgtccg tatgcggctc
    17941 gcgcgatgga agccattggt aaaccttttg agaaagccgg acgtttcgac cagcaatcga
    18001 tggtttgcgg tcagtgccat gtggagtatt acttcgacgg caaaaacaaa gcggttaaat
    18061 tcccgtggga tgacggcatg aaagtcgaaa atatggagca gtattacgac aaaattgcct
    18121 tctctgactg gactaactcc ctgtcgaaaa cgccaatgct gaaagcgcag cacccggaat
    18181 atgaaacctg gacagcgggc attcacggca aaaacaacgt gacctgtatc gattgccata
    18241 tgccaaaagt gcagaacgcc gaaggcaaac tctacaccga ccataaaatt ggtaatccgt
    18301 ttgataactt cgcccagact tgtgcgaact gccataccca ggacaaagct gccttgcaga
    18361 aagtggtcgc ggaacgtaag cagtcgatta acgacctgaa aatcaaggtt gaagatcaac
    18421 tggttcacgc tcacttcgaa gcgaaagcgg cgctggatgc aggcgcaacg gaagccgaaa
    18481 tgaagccgat tcaggacgat atccgtcatg cccagtggcg ttgggatttg gcgatcgctt
    18541 cccacggcat tcatatgcac gcaccggaag aaggtctgag gatgctcggt acggcgatgg
    18601 ataaagcggc ggatgcacgc accaaactgg cgcgcctgct ggcgaccaaa ggcattaccc
    18661 atgaaatcca gatcccggat atctcgacca aagagaaagc ccagcaggcc attggcctga
    18721 acatggagca aatcaaggcc gagaagcagg acttcatcaa aacggtgatc ccgcagtggg
    18781 aagagcaggc acgtaaaaac ggtctgttaa gccaataacc ccgttccgcc tcgcaagggg
    18841 cggaaaacac aatggagtga atatgagcgt attacgttcg ttattaactg ccggggtgct
    18901 ggcgtcgggc ctgttgtgga gcctgaacgg aattaccgct acccctgcgg cgcaggcatc
    18961 tgatgatcgc tacgaagtta cccagcagcg taacccggat gccgcctgtc tggactgtca
    19021 taagccagat accgaaggta tgcatggcaa acatgcttct gttatcaacc cgaataacaa
    19081 actgccggtc acctgcacca actgccacgg tcagccatca ccgcaacacc gcgaaggggt
    19141 gaaagatgtg atgcgcttta acgagccgat gtacaaggtt ggagagcaga acagcgtctg
    19201 tatgtcctgc catctgccgg aacagttgca aaaagcgttc tggccgcacg atgtccacgt
    19261 aaccaaagtg gcgtgcgcca gctgccattc cctgcatcca cagcaagata cgatgcagac
    19321 gttaagtgac aaaggacgga ttaagatttg cgtcgattgc cacagcgatc aacgcaccaa
    19381 tccgaacttt aacccggcgt ctgttccgtt gcttaaggag cagccatgac ctggtctcgt
    19441 cgccagtttc tcaccggcgt cggcgtgctg gccgccgtca gcggcaccgc aggacgcgtg
    19501 gtggcgaaaa cgttgaatat caatggggtg cgttacggca tggtgcatga cgagtcgtta
    19561 tgcatcggct gtaccgcctg tatggatgct tgtcgggaag tgaacaaggt gccggaaggc
    19621 gtctcgcgcc tgacgattat tcgtagcgag ccgcagggcg aatttcctga tgtgaaatat
    19681 cgcttcttcc gtaagtcttg ccagcactgc gatcatgcgc cgtgcgttga cgtctgcccg
    19741 accggtgcgt cttttcgcga cgctgccagt gggattgtcg atgttaatcc ggatctctgc
    19801 gtcggttgtc agtactgcat cgccgcctgt ccgtaccgcg tgcgctttat ccatccggtc
    19861 acgaagacag cggacaaatg cgatttctgc cgtaagacca atttgcaggc cggtaagctg
    19921 cccgcgtgcg ttgaggcttg cccgacgaag gcgctgacgt ttggcaatct ggacgatccc
    19981 aacagtgagg tttcgcaact gctgcgccag aagcccactt accgctacaa gctggcgctg
    20041 ggaaccaaac cgaagctgta ccgcgtaccg tttaaatacg gggaggtgag ccaatgacgc
    20101 agacttccgc atttcatttt gaatcgctgg tgtgggactg gccgattgcc atctacctgt
    20161 ttttgattgg tatttctgcc ggtctggtga cgctggccgt gctgttacgt cgcttctacc
    20221 ctcaggcagg cggtgcagac agtacgttgc tgcgcaccac gctgattgtc gggcctggcg
    20281 cggtgatcct cggtctgttg atcctcgtct tccacctgac aagaccgtgg accttctgga
    20341 agctgatgtt ccactacagt tttacctcgg tgatgtcgat gggggtgatg ctgtttcagc
    20401 tttacatggt ggtgctggtg ctgtggctgg cgaaaatctt tgaacatgat ttgcttgccc
    20461 tgcaacaacg ctggttgccg aagctgggga tcgtgcaaaa ggtcttgagc ctgctgacgc
    20521 ccgttcatcg cggactggaa acattgatgc tggtgctggc ggtgttgttg ggggcttata
    20581 ccggctttct gctgtcggcg ctgaaatcgt atccgttcct caataacccg atcctgccgg
    20641 tgctgttcct cttctccggc atctcgtccg gtgcggcggt ggcgctgatc gccatggcga
    20701 tacgccaacg cagtaacccg cattccacgg aagcgcactt tgtacacagt atggaaatcc
    20761 ccgtggtatg gggtgaaatc ttcttgctgg tggcgttttt tgtcggtctg gcgctgggcg
    20821 atgacggtaa agtgcgtgcg ctggtggcgg cattgggcgg cggtttctgg acgtggtggt
    20881 tctggcttgg tgtcgccggg ctggggctga ttgtgccaat gttgctcaaa ccgtgggtca
    20941 atcgcagttc cggcattcct gccgtgctgg cggcatgtgg ggccagcctg gtcggcgtgt
    21001 tgatgctgcg ctttttcatt ctctacgccg ggcagttaac ggtggcgtaa gccagaacag
    21061 aggtggtttc tggacgtatt ccttcctgaa gtcggttttc tggcattgtt gttaagtctc
    21121 ggggtcaacg tgttgacccc gttgacggcc ttcgcgggag tgcggttgcg ctggcctgcc
    21181 atgatgcgac tcacttgcat cggcattctg gcgcagttcg cgctcctgct gctcgccttt
    21241 ggcgtactga cgtattgttt tctcatcagc gatttctcgg tcatttatgt cgcccaacat
    21301 agctatagcc tgctgtcgtg ggaactcaaa ctggcggcgg tgtggggcgg tcatgaaggt
    21361 tcgctgctgc tttgggtgct gctgctttcc gcctggagcg cgctgtttgc ctggcattat
    21421 cgacagcaaa ccgatccgct atttccgctg acgctagccg ttttatctct catgctcgcc
    21481 gcactgctac tgtttgtggt gctgtggtcc gatcccttcg tgcggatatt tccaccagca
    21541 atcgaaggcc gcgatctcaa tccgatgctg caacatcccg gtcttatctt tcatccaccg
    21601 ctgctttatc tcggctacgg cggtttgatg gtggcggcga gcgtggcgct ggcgagttta
    21661 ctgcgcggcg agtttgatgg tacctgcgcc cgaatttgct ggcgttgggc acgaccaggc
    21721 tggagcgcat taacggcggg gatcatcctc ggttcctggt gggcctattg cgaactgggc
    21781 tggggcggct ggtggttctg ggacccggtg gaaaacgcct ctttattacc ctggctttct
    21841 gccactgcac tgctgcacag tttgtccctg acacgccagc gggggatttt ccgtcactgg
    21901 tcgctgctgc tggcaatagt tactctgatg ttgtcgctgt tgggcacgtt gattgtccgt
    21961 tctggtattc tggtttcggt ccatgcgttc gcgctggata acgtccgcgc tgtaccgctg
    22021 ttcagtctgt ttgtgttgat tagccttgcg tctctggcac tgtatggctg gcgagcgcgg
    22081 gacggtggcc cggcggtgcg tttttcgggg ttatcgcggg aaatgttaat cctcgctacg
    22141 ctgttgctgt tttgcgcagt gctcctgatc gtgctggtgg gaacgcttta tccgatgatt
    22201 tacggcctgc tgggctgggg acgcctctcc gttggtgcgc catattttaa ccgcgcaacg
    22261 ttaccgtttg gtctgttgat gctggtggtg attgtgctgg cgacgtttgt ctctggcaaa
    22321 cgcgtgcagc ttccggcgct ggtagctcat gcgggcgtgc tgttatttgc cgcggggatc
    22381 gtggtctcca gcgtcagccg tcaggagatc agcctgaatt tacagccggg tcagcaggtg
    22441 acgctggcag gatacacctt ccgttttgag cgcctcgatc tgcaagccaa aggcaattac
    22501 accagcgaaa aagcgatagt ggcgctgttt gaccatcagc aacgtattgg tgaactgacg
    22561 ccggagcggc gtttttatga agcacgtcgt cagcaaatga tggaaccgtc aattcgctgg
    22621 aacggcatcc atgactggta tgcggtcatg ggcgagaaaa ccggaccgga tcgttacgct
    22681 tttcgtttgt atgtacaaag cggtgtgcgc tggatctggg ggggaggatt gttgatgatt
    22741 gcgggcgcat tgttaagcgg atggcggggg aggaagcgcg atgaataaag ggcttctcac
    22801 gctactgctg ttatttacct gttttgcgcg cgctcaggtt gtagacacct ggcaattcgc
    22861 caatccgcaa caacaggaac aggcgttaaa tattgccagc cagttacgct gcccgcagtg
    22921 ccagaatcag aacttactgg aatccaatgc gccggtggca gttagtatgc gccatcaggt
    22981 ttacaccatg gtggaagagg ggaaaagtga agtcgaaatc attggctgga tgaccgaacg
    23041 ctacggagat tttgttcgct ataacccacc gttaacgggt cagacgctgt tattgtgggc
    23101 tctgccagtg gtgttgttac tgctgatggc actgatcctc tggcgagtga gggcgaagcg
    23161 atgaaacagc ctcaaatacc ggtgaaaatg ctgacaacac tcacgatttt gatggtattt
    23221 ctctgtgtca gcagttatct gttaagtcca aaatggcagg cggtacatgc ggagtatcag
    23281 cgtcagcgcg atccgctaca tcagtttgcc agccagcaga ccccggaagc tcagcttcag
    23341 gcattgcagg ataaaatccg cgctaatcca caaaacagcg aacaatgggc gttactgggc
    23401 gagtactatc tgtggcaaaa cgattacagc aattcgctgc tggcgtaccg tcaggcgttg
    23461 caactacgtg gtgagaacgc tgaactgtat gcggcgctgg cgacggtgct ttattaccag
    23521 gccagccagc atatgaccgc ccagacacgc gcaatgatcg ataaagccct cgcgctggac
    23581 tctaacgaaa tcaccgctct gatgctgctg gcttccgatg cgtttatgca ggcgaattac
    23641 gcgcaggcca tcgaactatg gcaaaaagtg atggatctca actcaccccg gatcaaccgg
    23701 acacagctgg tcgagtcgat taatatggcg aaattgttgc agcggagatc agattaatca
    23761 tctggtgatg aagattatgt tattgagctg cataaaaaat aatcgaatga acatatgcca
    23821 aaaataatca ccaatcagta ttattgcaga ttaaacaaat aaaaatcttt ccataacaaa
    23881 tggttattca ttaatcctgc cataaactcg cgttattatg cattaatgca gcgaaaagct
    23941 ctgttgttaa agggttgcgc aacataccgc gcaaatgata ctgatcataa gcgttaaaaa
    24001 aatctacaaa ccaacgcaac acaattcatg ccctggcagt atgtcacgtt ctcgcgtttc
    24061 tgaacgggga acggcgctcc attgaggaag ttattctgat gaaaaatata aaattcagcc
    24121 tggcctggca gattctgttt gctatggtgc tgggcattct cctgggaagc tacctgcact
    24181 accatagcga cagccgcgac tggctggtcg tcaatttgct ctctccggcg ggtgatatct
    24241 tcatccatct gatcaaaatg attgttgtgc cgattgtgat ctccacgctg gtggtgggta
    24301 tcgcgggtgt tggtgatgcc aaacagctcg ggcgtattgg cgcgaaaacc attatttact
    24361 tcgaggtgat caccaccgtc gccatcattt tggggatcac tctggcgaac gtcttccagc
    24421 ccggtgccgg ggtggatatg tcgcagctgg cgaccgtcga tatctcgaaa tatcagagca
    24481 cgacggaagc ggtacaaagc agttcccacg gcattatggg cacgattttg tcgctggtgc
    24541 cgacgaacat tgtggcgtcg atggcgaaag gcgaaatgct gccgatcatc ttcttctcgg
    24601 tgctgtttgg tctggggctt tcctccctgc ccgcgacgca tcgtgaaccg ctggtgaccg
    24661 tgttccgctc catctctgaa accatgttta aagtgactca catggtgatg cgttatgcgc
    24721 cggtgggtgt gtttgcgctg attgcggtga cggtggctaa ctttggtttc tcgtctctgt
    24781 ggccactggc gaaactggtg ctgctggtgc atttcgccat tctgttcttc gcgctggtcg
    24841 tgctgggaat tgtggcgcgc ctgtgcgggt taagcgtctg gatcctgatt cgtattctga
    24901 aagatgagct gattctggcg tactccactg ccagctctga aagcgtgctg ccgcgaatta
    24961 ttgagaagat ggaagcctac ggagcaccgg cgtcgatcac cagtttcgtg gtgccgaccg
    25021 gttactcttt taaccttgat ggttcgacgc tgtatcaaag tattgccgct attttcatcg
    25081 cgcagttgta tggcattgac ctgtccatct ggcaggaaat cattctggtg ctgacgctga
    25141 tggtgacctc gaaagggatt gctggcgtgc cgggcgtgtc gtttgtggtg ttgctggcaa
    25201 cgctgggcag cgtagggatc ccgctggaag gtctggcgtt tattgccggt gttgaccgta
    25261 tcctcgacat ggcgcgtact gcgctgaacg tggtgggtaa tgcgctggcg gtgctggtga
    25321 ttgccaagtg ggaacacaaa tttgaccgta agaaagcgct ggcttatgag cgcgaagtgc
    25381 tgggcaaatt tgataaaact gcggatcaat aattgaagat tgccggggat atccacccgg
    25441 caatatgtgg atgcctgatg cgacgcttac cgcgtcttat caggcctacg ccagactgcg
    25501 caatagcttg atttagggtg attttgtagg tcggataagg cgtttacgcc gcatccgaca
    25561 tcaatatctg atgcgacgct tgccgcgtct tatcaggcct acgccagact gcgcaatagc
    25621 ttgatttagg gtgattttgt aggtcggata aggcgtttac gccgcatccg acatcaatat
    25681 ctgatgcgac gcttaccgcg tcttatcagg cctacgccag actgcgcaat agcctgattt
    25741 agcgtgattt tgtaggtcgg ataaggcgtt tacgccgcat ccgacatcaa tgcctgatgc
    25801 gacgcttgcc gcgtcttatc aggcctacct taaccgttag ttaatttttc aaactcttca
    25861 cacccggtat caaacccttc catacagctc agatttaacc agtgcagcgc cttttgtttg
    25921 ttcttctcga taaaaccttc ttcaccgttt aagaacatca ttcccgccca gtactcggaa
    25981 taaccggttc gggaaatcgc agagctgcgt ttgaaatacc aggttgcctt gtcatcgtcg
    26041 gccttaatgc ccacgccgtt ggcgtaaatc aaaccaagca gcatttgggc atcgacggca
    26101 gagtcgttct ccagatcttc cgaagcgttt tctaacagcg aaatcgcctt tggataatcc
    26161 ggtttacccg cttgcgtatt taccagaata tgcgccagcg ttacttcacc ttctttactg
    26221 cccgcttgcg ctgctttctc tgcaagaact tttgcttgtg gatagtccag actgaccgga
    26281 ttggtgattt tgatctgcgc caacagcgcg caggcatcag catcaccgct gtcggcggct
    26341 ttctgtgccc aatattcggc tttgctcaaa tcgccggagc taaaccagct gtcggcaaga
    26401 aaatattgtg cgcgtcggtc cccggcctct gctgccttta aatactggct gccaggctcg
    26461 gagtcgttcg cgtgggcaaa gaatgttaaa aacaacatca atgcgataat ttttttcatt
    26521 ttgataatac gattgggtag ggagtaacca gtataaagag ttcgaatgga taaaaaaaca
    26581 gcctccgaaa ggaggctgta gaaaggactg tattacgcca gtgccgcttc gcgcaggcga
    26641 gttttcaact tgttgtactc gtcaatcacg tactgctcgg cggcgcgctg atcggcgatc
    26701 ggctcgacgc gcacggcgca gtatttgtat tccggcgttt tcgtaatcgg gcttaagttt
    26761 tcggtaacca gctcgttaca ggcaccaatc caccactggt aggtcatgta aatcgcccct
    26821 ttgttcggac gatcgctgac ctgcgcacgg gtgataattt tgcctttacg cgagtgcacc
    26881 caaaccaatg cctcatcttc aatacccaga cgtttggcgt cttcggtatt gatttgtgcg
    26941 tagccaggtt catcagccag cgccgccagt gccgcacagt taccggtcat cgaacggcaa
    27001 gagtagtgac caacttcacg caccgttgac agtaccatcg ggtactcgtc ggtgagtttg
    27061 tcgattggcg ctacccagtc gcaggtgaag aactgcgcca gaccgttcgg ggtatcaaac
    27121 ttctctttaa acagataaga agtcccctga tcggtatctg aagtatcgcg gcaaggccac
    27181 tgaatgaagc ccagttcgcc cattttttcg taagtcgcac cgtagaaatc cgggcacaga
    27241 tgacgcaact catcccagat ctcctgggtg ttgttgtagt gcatcggata acccatacgg
    27301 gtggcgattt cactgataat ctgccagtcc gttttcagat cccatttcgg ttcaaccgct
    27361 ttgaagaaac gctggaagcc acggtcagcc gcagtaaaca cgccttcatg ctcgccccac
    27421 gacgttgacg gtaaaataac atccgccgcc gacgcggttt tggtcataaa gatgtcctga
    27481 acgatgacca gttccagatc ttcaaaggct ttacgtactg ccgacagctc cgcgtcagtt
    27541 tgcagcggat cttcgcccat aatgtacgcg gcacgcactt cgccatgcgc tgcgcggtgc
    27601 ggcagctcgc tgatgcgata gccggtatga gcaggcaggc tttccacgcc ccaggctttg
    27661 gcgaatttct cgcggttagc cggatctttc acgtactggt atcccggata cgtatccggc
    27721 agcgcgccca tatcgcaggc accctgcacg ttgttctgac cacgaaccgg gtttacaccc
    27781 gcatgcggct taccgaggtt accagtcagc atcgcgaggc tggtcagaga acgcacggtt
    27841 tccacgccct ggtagaactg ggttacaccc atgccccaca ggatggcggc gcttttcgcc
    27901 tgggcataca tccgcgccgc ctgacgaatc tcactggcgc tgacgccggt gatatcttca
    27961 accgactccg gcgtgtagcc ttcaacgatt ttacgatact cttcaaagcc ttctgtacgg
    28021 gaagcgacga aagctttgtc gtacaggttt tcttcaataa tgacatgacc catcgcattc
    28081 aacagcgcga tgttcgagcc gtttttcagt gcaatgtgca tgtcagcaat gcgcgcggtt
    28141 tcaattttgc gcggatcgca gacgataatt ttcgccccgt tacgtttagc gttaattacg
    28201 tggttcgcca cgattgggtg ggaatccgcc gggttgtacc cgaaaacgaa cactaaatcg
    28261 gtattatcaa tttcgttaat agcattgctc attgcgccat taccgaccga ttggtgcaga
    28321 cctgcaaccg atgggccgtg tcagacacga gcgcagcagt caacgttatt ggtaccaata
    28381 acggcgcgcg caaatttttg cattacatag ttggtttcgt tacccgtacc acgcgaggag
    28441 ccggtcgtct ggatggcatc cggaccgtac ttctctttga tggcgctcag gcgctcggca
    28501 acgtaattca gtgcctcatc ccaggaaaca ggttcgagtt tgccgccacg ctggcgacgg
    28561 atcatggggg ttttcaggcg cggggtcagg atctgggtat cgttaatgaa gtcccagcca
    28621 taataaccct tcagacacag ggtaccctgg ttggttttcc cctgcgctgc ctccgcccgg
    28681 acgattttgc cgttatcgac gaccaggttg attttgcaac ctgatgcgca ataggggcaa
    28741 accgtgacga cttttttcat cggtctcgct ccagttaatc aaatcacgca tacgcgctct
    28801 cgactacagt atgcatcttt tatgccacat tttatttggg gtcattccct gatattacgg
    28861 gcactattta ttcaaaactc tgacgaaaaa caggctgtcg tcagttttga cgtgacgaaa
    28921 cgaaataccg cgtgacagcc atcacgcggc agacatttta ttttttctcg acgacgggag
    28981 ccgcctgata cccgccgccc agcgatttca tcaactgaat gctttggatc acccggcggc
    29041 tgtccagcat cagtaatgac atctcttcgg caagcactgg caaccgggct tcggtggcct
    29101 gtaagcggct ggttaagccg cgctgataag cggcctcggc tgcgcgctgg gtgaagcgcg
    29161 ttgcttccac gcgttccgcc tgcatctctc gctcgtcgtt gagggtttgc agacgtgtgc
    29221 cgttgacggc aacgtcacgc accgcgttca atactgactg gttgtaacgt tcaatcatca
    29281 tgttgctggc ggtgcgagtg ccttcgagat tggcattcaa ccgtccaccg tcaaacagcg
    29341 gcaatttcag acccgggatg aagttgaact ggcgactggt ttttttgaat aaggtatcca
    29401 ggtggatgga gtccagaccg aaaaacgctt tgatatcaaa gctcgggtag aacagcgccc
    29461 gcgcggaatc cacctgatct aatgacgcct gaacatacca gcgcatggct tgcagatccg
    29521 ggcgtctggc gagcaactca taagagagcg ttgccggaat gccggtctgt actcgtggta
    29581 atgccaccgg tttgatctcc ggcatatcgc tggctcccgc gccaatcaat gcacgcagag
    29641 attctcgcgt ttcagtgatt tgccctttga cggcagcaat ttgtttatcg accgccagaa
    29701 tctgtgcccg cgcgccgtgg aaaggcactt gcgcttccag accgtgcgcc actttactct
    29761 ggtgcgcttt caccgcgtaa tcaatcacat cgcgagtttg ttctaacaga tcgagcatct
    29821 gatagctggc ctgcatactg taataaagct gcgctacgcc cgtggtcagc gatagctcta
    29881 ctgctgcggt ttctgccagc gcggcattat gcgcgccaat ggcggcagca accgctgagc
    29941 gatgcacacc ccacaaatcg agatccagtc cggcaaacaa accgactgtg gcttccgtat
    30001 agtacggccc gtccataccc agtgctggcg catccatcgc ataagggctt aaaaagccgt
    30061 tcgccgagac gcgttggcgg ttgagcatcc ctaacgctgc gacctgtaat tgtgaaccgg
    30121 catctaacaa atcggcctgc gactgcgctt tttcttccag cagtttcgct tcggcgaggg
    30181 tgtgtgaacc acttagcgtc cgttggatca gggcatccaa ctgcgggtca ttgagttgtt
    30241 tccaccactg cgcctgcggc cagccggagc tggcaagatg aatatcatcg gccagtttga
    30301 tttgttccgg tttgagctgt tgatgaggcg cagaatcctt acgtaccagg gcacagccgg
    30361 aaatcagcgt cgtgctgccg agaatgctgc acaacagcag gcgtgaaagt tgacgattga
    30421 tcattgcgtg gctccttgta cctgttccgt ggcgggcgtt aatgccgggg ctgtccagtc
    30481 tggcaagcgg gcgaaaagct ggcagacgtg ttgctcctgt tttaataagg tgggcaggat
    30541 ggcctgcgag gcgggtgtct cttcaagcgt tatttgagga gaacggctga gtgcggttgc
    30601 cagaccggcg gcgtatttct ccagggcgtc ggcaaactga cctgctctgt ccggctgtag
    30661 tgcgttatcc agcgcctgcg ccgagttcca ggttgcatcc caggcgtgaa gaatatcgcg
    30721 gccctgacga ataaccgttt gcgaacgttc aatcagtaaa gcgcgttctt cgctgtccag
    30781 ttgacgctcc agcgccacgc gttggcacat ctcttcacag gcattaaacg ccgcatgcag
    30841 acctatgcga atttgcagaa aagtgcgcag agcagtgact tcctgctggc gtgggatccg
    30901 cattacttta ctcaacatac ccagcgcgcc agccagtttt tgcggcagcg tgcgcgcttc
    30961 actttcaggc cagacaaagg tgtaaatcac cgcggacacc acggtaccaa tgatgatacc
    31021 tagggcgcga tcgcgaattt ccaccaggtc gtacaccggg ccaaaaacgt tttcgagcgt
    31081 ggcgagcgcg aaggtgacca ccatctgtgt gccgatataa gaagagcgtt cagagctggt
    31141 ggcaatccat gcacccaaca ggaaaatcgg tgccagcaca aatagcaatt cgacaatatt
    31201 gtccagccag ggcatgacca gcagcgtgaa taacagcgcc agaattgcac cgcagaaggc
    31261 accgccaaaa cgcagcgcca ttttctgata cgacgaacca acattcgggt tagcgacgat
    31321 cacgcaggtc agcatacagg tgtgaatgcc ttcccagtcc acgccgctgt agaaggtgta
    31381 acagatcaaa caggcgagca gcgtttttac cgcgtagcgc atatagtctg gattggtaaa
    31441 agcgtcggcg accattgatg gcggtttggt ggcgggcgtt ggcggcgtat tcgggtccat
    31501 ctgacccagt tgcaataacg tctggcagat attctccagg ttacattccc gtgccgccat
    31561 cgcttcactt tcagtgatcc gccagtcgct ttgccagcac tgaccttcgg caatggcatg
    31621 ctgcagcttg ttgatttctg aggctaattt ttgccgaaat tcaataattg cctgagaatc
    31681 agcaaaagag gtgggatcgt agcgattcag cgtcgagtaa atgtaggtta ccgttgccac
    31741 gcagcttagc caccatgcgc tctgagttcg ccagttggca tcgtccgcga ggcaaaagac
    31801 attgagtttt tgcagcgcca gcgcctctct ttcaatccgc gtttcgggta gcggtgcgag
    31861 gctgtccgtc aggtggctaa tggcatcatc aagccgatca ttaagcgcct gatgcatctg
    31921 cgtgatagcg cggctgggaa accacagcac gccgattaac gtcatcagca aggttggata
    31981 gaggccgaca acgatacacc acagggttaa gcgcacgacc acttccggat agtcgagcat
    32041 ggcggggaag gtttgcccgt aaatagcgac tatggcgacg gcgaaaaaga ccagccccaa
    32101 ccgatgggtg cgcatcaaaa acatacagcc catcaggatc ggtccggcga tgatcaatcg
    32161 gatcaacggt tcgccgtatg accatttgta gatcaaaaac aggctgccga tctccagcac
    32221 cgtggcaacc acaaacaaga tcgcgacaaa tttggtgtaa aacgcgttcg actgaatacc
    32281 gtaaaacagc actgccagcg ataacgccac aaaagggatc tcaaaggtca tcgagatcag
    32341 aattaccagc aggcagccta cccagagttg cacggtctgc ggcacgcgac ctggccgccg
    32401 ctcgcttaac tcttcatgaa agaacgccag cagcctgacc accggtaatg gcagggagtt
    32461 gagcgcgctc attattgcgg ctcaagatta gcgactgccg aagcgccgat gcggaacatt
    32521 tccgggtcag gtttatcgac catgattttg actggaaaac gctgggcaac gcggacccag
    32581 ttaatagaac gagacacttt cggcaggccg cccagcacca ggccgccgtc atccggtagc
    32641 acgccgtagc caatcgaatc cactttaccc tcgaaggttt tgccgctgtc gctcatcagg
    32701 cgaatggttg cgggtgtacc tgagcgaata tttttcagat cggtttcgcg gaagttggcg
    32761 atcacgtacc agtgacgagt gtcgattagg gtaaaaatag ggcgcatggc agaagcaaat
    32821 tgcccgacgg aggttttgag ggaaatgacc cggccatcaa acggcgcgcg aacggtcgcc
    32881 atttccagat gcagtttggt cagggcaata tccgcttcga ctgccgcacg ctgcgccacc
    32941 agcgcatcca cgccgctgac ggcgctggcg gctgactgcg cctgcaacaa tacggcatta
    33001 agatccgctt ctgccgcacg ctgtgcggtt cttgcacggt caacctcttc cgcagaaaca
    33061 aaaccttctc gcagtagcgg ttcggtacgg cgtaacgtat cgctggcttg tttcgcggcg
    33121 gcacgggctt tttctaccgt ggcattaacc gagtcggcac caaactgttg tgcgtcaacg
    33181 ctacgctggg tgagcataat ttgcttatcc agcgccgcga gggaggcttc agctttcgcc
    33241 agattggctt cgtacgggcg cggatcaatg cggaacagca aatcgccctg tttcaccgcc
    33301 tggttgtcgg tgaccgccag ttctacaatg cgaccgctga cttccggcac cacatcaatg
    33361 gtatctgctg acgcgtaagc gtcattagtt gatggcgcac tgtctacgcg ccagataacg
    33421 aaaacaaggg caaccagcgc caacgcaacc actaacagag cagggaattt actgcgagga
    33481 gcttttttcg gcgtactttc catgagcgcg tctcaaatag attagaaaaa tgccagccag
    33541 aacagcatgg cgtagagagc aaaaagggcg gtataaataa ttccgacaaa tgccagattg
    33601 atatttgtcc gctgaataat tcgcctcgtg atgagcgtca aaataaggct ggcaatggcg
    33661 cagaaaaacc agccgggata ataagcgccg atcaccggga ttgccggaga taatgagcat
    33721 cccgaaacca tgaagacggg aattatccag gctgtttttt tgagttgcat cgccatgcga
    33781 gagagaactg ttggcatatt aaatacatcc tgtctgctgc gaacaatatc tgtagtgaat
    33841 atcaatatcc attgaaggcg tcagtatggc tattttctcg atgaataaaa atagctttat
    33901 tcagc
//
LOCUS       DAPHCV010000051        33039 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.051, whole genome shotgun sequence.
ACCESSION   DAPHCV010000051 DAPHCV010000000
VERSION     DAPHCV010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33039
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.051"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(383..673)
                     /locus_tag="RH033_002364"
     CDS             complement(383..673)
                     /locus_tag="RH033_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV2991540.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(674..985)
                     /locus_tag="RH033_002365"
     CDS             complement(674..985)
                     /locus_tag="RH033_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDV2991541.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            1214..2122
                     /locus_tag="RH033_002366"
     CDS             1214..2122
                     /locus_tag="RH033_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV2991542.1"
                     /translation="MALEKGIAELVEEFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEAMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAELVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(2186..3127)
                     /gene="fabY"
                     /locus_tag="RH033_002367"
                     /gene_synonym="yiiD"
     CDS             complement(2186..3127)
                     /gene="fabY"
                     /locus_tag="RH033_002367"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HDV2991543.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(3172..3609)
                     /gene="dtd"
                     /locus_tag="RH033_002368"
     CDS             complement(3172..3609)
                     /gene="dtd"
                     /locus_tag="RH033_002368"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDV2991544.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(3606..4478)
                     /locus_tag="RH033_002369"
     CDS             complement(3606..4478)
                     /locus_tag="RH033_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HDV2991545.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(4472..5071)
                     /gene="yihX"
                     /locus_tag="RH033_002370"
     CDS             complement(4472..5071)
                     /gene="yihX"
                     /locus_tag="RH033_002370"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HDV2991546.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(5212..6099)
                     /locus_tag="RH033_002371"
     CDS             complement(5212..6099)
                     /locus_tag="RH033_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HDV2991547.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGSATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQVGPWD
                     DCFGFYDGVKVKLLWPGKLAMTMTSSANSLVVFDKQPDATCINPLTQAPNAINLTPEF
                     VTPDKPLVIETRWQFTPES"
     gene            complement(6089..7438)
                     /locus_tag="RH033_002372"
     CDS             complement(6089..7438)
                     /locus_tag="RH033_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HDV2991548.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIASFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            complement(7537..8970)
                     /locus_tag="RH033_002373"
     CDS             complement(7537..8970)
                     /locus_tag="RH033_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2991549.1"
                     /translation="MSQITSTATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            complement(9040..10050)
                     /locus_tag="RH033_002374"
     CDS             complement(9040..10050)
                     /locus_tag="RH033_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDV2991550.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQQLKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATINDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            complement(10083..10970)
                     /locus_tag="RH033_002375"
     CDS             complement(10083..10970)
                     /locus_tag="RH033_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDV2991551.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            complement(10995..11873)
                     /gene="yihU"
                     /locus_tag="RH033_002376"
     CDS             complement(10995..11873)
                     /gene="yihU"
                     /locus_tag="RH033_002376"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HDV2991552.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGSLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARKTGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            12046..12942
                     /locus_tag="RH033_002377"
     CDS             12046..12942
                     /locus_tag="RH033_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDV2991553.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLACLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            12982..13770
                     /locus_tag="RH033_002378"
     CDS             12982..13770
                     /locus_tag="RH033_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDV2991554.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(13830..15095)
                     /locus_tag="RH033_002379"
     CDS             complement(13830..15095)
                     /locus_tag="RH033_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2991555.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASKNTSMTSVLKD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(15197..16177)
                     /locus_tag="RH033_002380"
     CDS             complement(15197..16177)
                     /locus_tag="RH033_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HDV2991556.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEEGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNN"
     gene            complement(16185..16895)
                     /locus_tag="RH033_002381"
     CDS             complement(16185..16895)
                     /locus_tag="RH033_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HDV2991557.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(17113..18936)
                     /gene="typA"
                     /locus_tag="RH033_002382"
     CDS             complement(17113..18936)
                     /gene="typA"
                     /locus_tag="RH033_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HDV2991558.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            19309..20718
                     /gene="glnA"
                     /locus_tag="RH033_002383"
     CDS             19309..20718
                     /gene="glnA"
                     /locus_tag="RH033_002383"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HDV2991559.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLA
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            20892..21941
                     /gene="glnL"
                     /locus_tag="RH033_002384"
     CDS             20892..21941
                     /gene="glnL"
                     /locus_tag="RH033_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HDV2991560.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            21953..23362
                     /gene="glnG"
                     /locus_tag="RH033_002385"
     CDS             21953..23362
                     /gene="glnG"
                     /locus_tag="RH033_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HDV2991561.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            23474..23584
                     /locus_tag="RH033_002386"
     CDS             23474..23584
                     /locus_tag="RH033_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HDV2991562.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(23774..25147)
                     /gene="hemN"
                     /locus_tag="RH033_002387"
     CDS             complement(23774..25147)
                     /gene="hemN"
                     /locus_tag="RH033_002387"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDV2991563.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEQQW
                     DLLFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(25336..25845)
                     /gene="yihI"
                     /locus_tag="RH033_002388"
     CDS             complement(25336..25845)
                     /gene="yihI"
                     /locus_tag="RH033_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HDV2991564.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            26427..27059
                     /locus_tag="RH033_002389"
     CDS             26427..27059
                     /locus_tag="RH033_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDV2991565.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFNEMQPVEETQDGE"
     gene            complement(27441..30227)
                     /gene="polA"
                     /locus_tag="RH033_002390"
     CDS             complement(27441..30227)
                     /gene="polA"
                     /locus_tag="RH033_002390"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDV2991566.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAARLQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQSIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            30591..31523
                     /locus_tag="RH033_002391"
     CDS             30591..31523
                     /locus_tag="RH033_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HDV2991567.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCECLAVLLHLNPYLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(31564..32994)
                     /locus_tag="RH033_002392"
     CDS             complement(31564..32994)
                     /locus_tag="RH033_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HDV2991568.1"
                     /translation="MIRKSATGIIVALAVIWGGGTWYTGTQIQPGVEKFIKDFSDAKK
                     KGEHAYDMTLSYKNFDKGFFNSHFQMQITFDNGAPDLNIKPGQKVAFDVDVEHGPLPI
                     TMLMRGNVIPALAAAKVKLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AKYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNTAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVATEIAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVAFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         8315 a         8478 c         7920 g         8326 t
ORIGIN      
        1 tttttgattg gatctttatt tgcataatct gaatggtgta gccgaaaaat gtcagcatcc
       61 taacagcacc cggatttaag gtgaaattaa tcatccatca cagaccggtc agtactgcta
      121 ccaaccggtc ttaacttgct catcgttata attttgttgg taacagttcg ggaaacaagg
      181 aattccttcc ggaacacccc tgacatcatc cgatagcatt gacgtcaatc atcggcgcga
      241 cgatgagttt ttttaatcgc atcatagggt ggcaacggtc agcacgatta cgggtcatag
      301 caaaactcct gagcaggtgg cactacgccg ctttcacagg agttctgaat gctggtgctt
      361 tctgtatggc ataaccatgc ctctaacggt taagtgcttg cagcacatgt tgcggatcat
      421 tggcaatcgc tcgcagcaaa gcctttgcag gcccggtcgg tgaacgtcgc ccctgctccc
      481 agttacgcaa cgtgtctacg cttacagaaa taagatcggc aaatttagat tgtgacaacc
      541 ctgaagcgcg gcgtatttct ttgattttca tagcatcaac ttcaaaagtc cgcgaaggct
      601 gtcgcgttcc ttcaataatt tcattcatct gggccatgct gtgagtcaac cgctcaaaaa
      661 gtgccttatc catttaccac ctcgtattta acaaccggag cattgctttt tcctggggag
      721 acagatcatc tttaataccc ttacgataaa taagcagtaa tctgatctca taatgtttaa
      781 cctgatgaaa gtaaataacc ctgacaccag cacgtttccc ctttcctcct gacacccatc
      841 gaacctttct taacccacca gtttcaggta ttacctcacc atagtcagga ttcaaggcca
      901 ggaaaaactg aaaacgactg aattcatcgt cggtcagcag tttttgaaca tcttcagtaa
      961 aaatttccgt ttcgatgaag agcataatta tacgccattg gcttagagta acaatccttt
     1021 ttgtacgtca ttgacgtacc cttgaaacca ctagcttact gatagtgctc cagcaaaacc
     1081 acgtctctgc cgggtttgtc tttacatttg cgaggcgctt tccagatttt tcacgcccgt
     1141 gaatttccct gtatccatcc agtaaaaacg ggtgtataaa actgtacatt catacagcaa
     1201 aatgagttct actatggcac tggaaaaagg aattgcagag ttagtcgaag agtttatcgc
     1261 tgcgggtcgc ccctcatcgc gtcaacaaag tattgcgcag cgcagggagg gctatatcgc
     1321 cagcgctgtc cttgcgggtg agacagaaac gcgggtagat attcagacta tcgaacttga
     1381 agcgatgacc ttacgaatag tgtcaccgct gaatgccccg acattactcc cgacgataat
     1441 ttattactac ggtggctgtt ttgttagcgg cgggttcgca actcacgaca accagctgcg
     1501 ccagttagcc tattacgggc aatgtcgggt tattgccgtc cagtatcggc tggcaccgga
     1561 acatactttc cccgccgccc atgatgatgc gcaacgaggt gcagaattag tctggcaaca
     1621 cgccgaacga ctcggcgttg ataaacaacg gataactctt gccggtgaca gtgcgggcgg
     1681 acatctggcg ctggtaacag ctttgcgatt aaagcgtgct ggcgagtggc aaccggcaca
     1741 actcatctta atttatccca tgcttgatgc gactgcgcat ttcgaaagct acatacgcaa
     1801 tggtcacgat tacatcatca cacgcgacac tttactgagc ggctttgaga tgtacctgcc
     1861 aggcattgaa cgcaggcatc ctgaagccag ccctctttgg cgcgatgatt tcaatggctt
     1921 gccacctgta catatcatta ccgctgaata cgacccactt tgcgatgaag gtgaagcgtt
     1981 gtatcaccgc atgacagggc aaggagtgca atgcacctgc caacgctacc ttggcgttat
     2041 ccacggattt tttcagttag gtggtatcag tgaggctgcg cgatcggcgc tacgggatgt
     2101 tgcctggaga gcgggaaggt aactccggac catggcccag aaaaggtttt cagattgaaa
     2161 accttttcag tcaagtaaaa cgcccctact cttcttcgtt cccgccctct tcatacgggc
     2221 caaatggctt cgcgggcaga acgatatacg tgccttcaaa cactgcaccc ggcgtctcgt
     2281 cgccaaagat ttctacttgc atctgcaccc gtgcttttcg tccgcgcgcc agacggtcga
     2341 gatcgccgct taaggcacca aggtcggcta ccgcatgagg tttgccgcta atcggcttgc
     2401 tatagcggat atgcgcatcc gccagaataa tcgttccgcc gaggtggcgt tcgcgcagca
     2461 tcagccagat aagcccccaa ccggtgagcg ttgccagcga gaataaactc ccggcaaaca
     2521 gcgtgtgatg cggattctga ttgcctgttt ccggcatggt ggtgataaat ttttgcccgg
     2581 tatattgctg aatgcgcacg cccatttttt cactcagcgg gatgtgttca taccacgcct
     2641 gttgcagctg cgcgcaccag tcgccgcgat gcaaaatatc atccagagtg gcaacgggtt
     2701 taatcatcaa aaaatggcga atcggcgtgg tggttggcgt ggtgatttct ccctgattaa
     2761 caaaccccag cttggcgaaa aactccaccg cgtcttcgcg ggcgctacag gtcacgcgct
     2821 taacaccttc ctgacgcgcc acagattcga gggtcatcgc catcagcgtt cctaaccctt
     2881 tgtcctgcac gtcgggatga acggccataa agcgaatgga cgcttcattg tcggcattaa
     2941 tatacagtcg gcctaccgcc accagattac cctgctcgtc gacgaccatc tggtgatgcg
     3001 ccatcgcatc ccacgcgtcg cgttccgaac ctttcggttg atgcaggggc ttacgcaaca
     3061 tttcccagcg aaactgatag taacgctcta attcttcttc tgtttgtgga acccgaaggt
     3121 gatacatagc gatactctct cttgtttccc gtgacaaccc tggaagctgg ctcatacctg
     3181 caaccagaat gtcacggggc catcattgac cagcgatacc tgcatatccg cagcgaagcg
     3241 tcctgtttgc gtgttcatct cttgctgacg gcagcgttcg acgaaatagt catataacgc
     3301 ctctgcgcga tccggtgatg cgcctttgga gaaacttggg cgcatccccc gttcggtatc
     3361 tgcggcgagg gtaaactggg aaaccaccag tacactgccg cccgcctgtt gcacgttgag
     3421 attcatcttg ccttcggcat cgctaaagat gcggtagccg agcacacgct cgcacagacg
     3481 gtttgctttc tgttcgtcgt catccttttc gacacccaat aacaccaaaa gtcccgcgcc
     3541 aatttcgccc gtcacttctc cctccacggt gacgctggca cgggttacgc gttgaattaa
     3601 tgcaatcatg gttcgtcgtc ttcttcttgt tcagcagctt gttttaattt gcggtattcc
     3661 ccgagagtga cagtaatttc cgcgccaagc aagacgatac accatgtcca gtagacccag
     3721 acaaagagaa tggggatcac cgccagcacg ccgtaaatga gctgatatga cgggaacatg
     3781 gtgatataaa gcgcgaaacc tttctttcct gcttcgaaca ggagtgcagc gacaaacgcg
     3841 ccgacaatcg cgtcgcggtt aggtacgcgg atggtaggaa caatgctgta cagcaaccag
     3901 aaggagatcc acgacaacag cagcggaaaa atacgcagca cgttgtcgat gacagtattg
     3961 agatcgctcg cccagcgcag ggagagcaga taggaactga tcgccagact ggcccctgcc
     4021 agcagcggcc ccagcgttaa aatcatccag tacacggcaa acgagtaaat ttttggtcgc
     4081 gctcgtttac tgcgccagat ggtattcaac gcgctatcga tggagtacat caacaataac
     4141 gccgtgacga tcagcccgca cgccccgacg gcggtcattt tgttggaatt ggcaacaaat
     4201 tgttcgatat accgctgaat aacatcgcca gtagcaggca gaaagttggc aaaaataaag
     4261 tgacgcaact gaatgctgac gtcggaaaac atgggaaaag cggcgaaaag cgcaaaaaca
     4321 acggcaacca gcggcactaa tgagagcaac gacacatagg caaggttacc tgccagggtt
     4381 gtcatgttgt cctcatcaat gcgttgccag agtagtttta gccaggccca tagtggacgg
     4441 gtacgatgcc tggctttgtc ctgaatggtt tttagcataa caccttcgcg aaatagtccg
     4501 ggatggtggt tttatctttc accagaatac tggtaatgcc tagttgatta gctccttcta
     4561 tattatcggc gttatcgtcg aaaaagaccg tatcgctggg tgaaaaacct tccgcctgca
     4621 aaacatgctg gtaaattcgt gcttcgggtt tgcgcatccc cagatcttgc gacagataga
     4681 tatggtcagc agcatcacga atttccggat attcttccgg ccagaaggtg gtatgcaggc
     4741 ggttggtatt ggaaagcacc accacgcgat gcccctgctc acgcagttta tgcatgatgg
     4801 cgatcacttc cgggcgcagc gcgacaaaca ccgcctgcca gccgtgagag aactgctcgt
     4861 agcttagcgg cagggccatc tcatgacaca gcgcctctgc gaacgcttcg tcgctaattt
     4921 ccccacgctc atgctgatga aacgcctcgc ccatgtggaa actcttctta agcgtagcca
     4981 gtggaacacg cgttaaatcg ctccaggccc ccagcacacg gttaaagtcg atatcaacaa
     5041 tcacattacc taaatcaaag atatagagca tttttgcctc ctttcgcgcc acaagaaatt
     5101 aactgtagcg ggaaaggatg ggtttggcta tgtggcaata agggtaatct tgtgatgctg
     5161 attgcgaaaa agcagagtaa tttcactggc tttttcgcaa tagatgaatg gttaagattc
     5221 aggtgtaaat tgccaacgag tttcgatgac cagcggctta tctggtgtca caaattccgg
     5281 cgttagattt attgcgttag gtgcctgagt cagcgggtta atacaggtgg catcgggctg
     5341 tttatcaaat accaccagac tgtttgcaga agaggtcatg gtcatcgcga gtttaccagg
     5401 ccacagtaac ttaaccttca ccccgtcata aaaaccaaaa cagtcatccc agggaccgac
     5461 ttgaggagag atacgatttc cagttggcag ttcatcacta cccgcttctt cttgccagtc
     5521 cgcatcaaaa agcacctgca acgattctgt attttgcgga gtcagctttt tcgcaaacca
     5581 cggatgccag cctgcggagg ccgggaatgt atcggcatat gaatggactt ccagttgcaa
     5641 aaccagcgca tcgttctcca gcaaaaaagt ctgaatcact tccccttgcc agggccacgg
     5701 tgaagccagg ggcatacgta aagtcaggga atctgctgtt ttgtcgatga tttcccacgt
     5761 tgagtaacaa gccataccat gcaacgcatg tggtggttta ttggcgggta aagaataaca
     5821 ttgcccacca gcattcagcg tcgcactccc cagtcgccct gcccacggca ccattgggaa
     5881 acagccatac tgaaaagctc tgcgctgcgg ctgccattgg cgtaatactt cagaaccaaa
     5941 tgcctttaac gaggttatcc tggcaccatc atgcggataa atctgcaggt gaatattgcc
     6001 tttttccagc gtcactaact ctcctacagg cccaggccat ggcgcgtcaa acgcctgttg
     6061 tagcgccagg taatgctcgt tttttcgttc agttttcatg agattgttcc ctgatgtagc
     6121 ggtaagaagc ggtttcttgt tcatcggtcg gctcaatcag ccagacgcaa acaaaggaaa
     6181 gaactgagac agcaaataaa aacagagcga cataccacgg ttttcctccc cctagcgcca
     6241 gcaaacttga ggcgataagg gggcttagtc cgccaagaat cgcggaaaat tgataagcaa
     6301 aagaaaggcc ggtatatcgt actttggtac cgaacatgcg ggtgaaaagt gttggctgca
     6361 cggcaaacat catcgtcgga cctaagttgt agccgaggat catactgcac cagatgatga
     6421 gcgtactttt gctatccagt aataagaaga aaggaaacgc gaacaggaca caaaatcctg
     6481 ctcccgacaa atacagactt ttttgaccta tacgatctga taaccatcca accaatggac
     6541 aactgaaaat accgatcgcc gaggcaatca acatacccgt caggggaata tctcgcgaca
     6601 atgctaattg gctggaaata tagacaatac caaaggcgtt aataatatta gaggagacac
     6661 tttccgccag cctcgcaccc agtgccaata aaatatttcg tggatgtttt ttgaataatt
     6721 caatcaacgg aggaacggac ttttctttat tattcgtcgt tttttgcttt tgaaaatcca
     6781 gcgtttcttc ggtatgcagg cggataaaca gcccaacgat caacatcacc gcagaaagcc
     6841 aaaacggaat gcgccatccc caggagagaa actgctccga agtaagaaaa tggttacaga
     6901 gagcaaaaat accggttgcc agcataatac ccacagacgc agccgtttgc gggagtgaac
     6961 ctaaaaatcc gcgtcgggat tcgggcgctg attctatggt catcaatgcc gctccgccgt
     7021 actcgccacc aagaccaaag ccctgaatca aacgcagcac cattaataca agcggtgccc
     7081 aaatgccaat ttcctgatac gtcggaataa aaccaatcaa aaatgtagag caaccgacaa
     7141 tagccagcgt ccagataagt gttatttttc gcccgatttt atcgccaaaa tgaccaaaga
     7201 cgatgccacc taaaggacga gtcagaaaac caacaccaaa ggtcgcaaag gaagctataa
     7261 gacctataaa aggatcgctg tctggataaa acagcggtgc aaataccagt cctgctgccg
     7321 tgccaaaaat aaagaagtca taccattcca ttaacgcgcc tgcgaaactg cccataacga
     7381 cacgtctcat ggctttaccg gaaagccctt cacgcggtgc gggtaacttt tcagacataa
     7441 ttttcctctc ctggtaggcg agaaatctgg gatacgaacc actaaacgtg ggcagtttga
     7501 attaatgcgc atcggcttaa accgatgcgc attaaattat ttcttcaatt cagccagaac
     7561 ctctgcgtcc tcagcatcaa catttgcacc cagtggttcc caattaatca atacacaggt
     7621 caggaatccc aggaatggca cgattgccaa ccagtaaaac gcttcagttt tcaacgcacc
     7681 ccacagcatc ggccagaaaa tgagtcctgc aattgcccct gtgcgcataa tagcgcgggc
     7741 aaatccgacc ccagtagggc gaatagcggg aggataagag agtgtggcaa tggtcatgcc
     7801 cagcccacca ggacccgctg aatggaagaa aataattgat cccaataacc ctaaagacag
     7861 ccagggatta gtcgttgcca gtgcgcctaa cgacagcaat gctaaggtca caacagcaaa
     7921 tccatacatc gactgtcggc gagtacccag tcgttggaga ataaacgagc cgatcatccc
     7981 acctgtcacc ccacaaaggg cattcacgac ggcagaaccg gtcaacgcgc cagtaagccc
     8041 gccacttatt attcccgcca acgtaagagg aagataaaca cctacggcgt tatattgcca
     8101 cgcctgcatg gttgccacga cacagccaag aatggtgcgt ttgcggtaac gatcattaaa
     8161 taagatgcca tatccaccac tgtatttatt ctctgctttt tccgagaatt tcgcttctgt
     8221 tgtacccgat gccgcaacgc gagcctgaac accataacgt tttcccagaa tgtcgcacgc
     8281 ttcctggtaa cgcccaacgc gtgcggccca cattgcggat tcaccaataa agaagtaacg
     8341 caaaatcatg aagatgactg caaaaatagc tccgatgaat attccgtaac gccacaattg
     8401 ttcttcagcg ataccagaca gaagcagcgg caaaagcacc aaataaacaa cggttgtcga
     8461 aacataccag gccatttgcc agaggttgac cgacgttccg gttttcttcg acgttgaacc
     8521 acgaagctct gccacggcat tagttgccaa agggaagtcg atgcctaaac caaagcccat
     8581 gatgacgcgg cacaccagca cccaccagat actgggagca atagctaccg ctgcagcacc
     8641 gatggtgcac agccccatgc caataataaa tgcccgcttt tgcccaaagc ggtttattac
     8701 tcggttcgct aataaaccgc caattaacgc ggcgattaat actgatgcat tgacggttgc
     8761 agcaagaccc ggcgaaatgc cgaattgagc ggcaatgtat ttattaccaa accccaccat
     8821 cgccgcctgg taggcatcaa tcaatatgcc gcccaaagca ataaaaataa cgccgatact
     8881 ggtgttgatt gcagagttct tattaacaat atcaatcacg tcctgtggcc gactaattga
     8941 ataagttgca gtactggtga tttgactcat cgcagtgtcc tttttaattg ttgtagggtg
     9001 gcgctgacgt gacgcagcgc cagacagaga tttagaaaat cattccggcg caaattgcag
     9061 catgaccttt atggagtcat tttgctgcgc gcgttcgaat gccgccgctg catcctcaat
     9121 ggcaaataaa tcggtaacca gttcactggt atcaaaccgc ccatccgcca gccattcgat
     9181 aacagcatcg aaatcttcag ggatgtacat gccagagttg agcaagtcac gctcgaaacg
     9241 ttgcatcatc ggtaatggta tttcacgcgg cccacttggt acgcccatgc acacgacggt
     9301 gcctccggca tatacacgag tacatgcatc gttaatcgtg gcctgcgctg caacgacatc
     9361 aatcgccccc gtaaatcgca catctgcggc cagctcacct ggtgcccaaa cttctgctgc
     9421 gcccagtttt aacgccagcg cgcgtttggc agcgtcaggt tcaatcacgg taatcttacc
     9481 tgcccccata atgcgcagtg cctgcacgat ggataagcct atcgttccgg caccaatcac
     9541 cagtacgctt tccagagaag ctttcggcat tcggttaacg cagtgacgag cacaagccgc
     9601 cggttcggca aaggccaaca cttttagcgg taaagaggct ggtgcgacat ggcaattacg
     9661 cgcaggaaca atgtgttgtg agcgagcaaa gcccggtgcg cgaaaaccag caacctgcgg
     9721 tggttcacaa agattaaaac gtcctgcttt gcaggctcgg cattccatgc aagccatgat
     9781 gggatcgacc acaacatgat cgcccggctg tacattttta acgtcgctgc ccacttccgt
     9841 aacgcgcgct gcaatttcat gaccggggac gacaggcggt ttggcaaacg gatgaccgcc
     9901 tttcagaaca tgcagatccg aaccgcatat cccgtagaac acgggcgcaa tccgtacttc
     9961 gtgcggttta agttgctggt gtggcacttc aacatattga gtggtgactt tcccaatatc
    10021 tgaaaataaa acctgggtaa ttttatccat tgttattctc cagaatagat atccataggg
    10081 gattagcgtt gcgcgatacc ttcatcgaca atttcagcca gccgttgcaa gcgtggaacg
    10141 gagacatcgc gcagcatggt ttgtggatct tgtgcaccaa caaccgacgc ccaaaccgct
    10201 cgtccagcca ggaagcccga cgcaccacct ttcatggcaa tacttactgc acggccaaaa
    10261 atatcggcat caacgccaga agaaagaatt acccacggca tcttcatctg atcgttaagt
    10321 tgctgacagg cggcaagtaa ggttttttca tcgcctttgc cccctaatgg catctcggct
    10381 ttgtaaagat cagcctctgt tccaccaagc tctgcggcgg cggcaacaat ggcgctttca
    10441 cgatcaaagt cccaaccgcg acgaggtgga cgcaccacag gttcaataat gctaaccagc
    10501 ccggcactac ggcagcgacg aacaaattta tcaaccattg ccagacgttc ctctgcgggt
    10561 tcatcttcac gccagagtac aagtaatttc attgccttag cccccatttc acgggcttta
    10621 tgcgggtcga cggacatatc tatttcaacg ctatcaaccg ggatgccatt acctgggata
    10681 aaaagatcgg cagcgacaat caggccgcaa gaattagcca cagcgccctg ctcgactatt
    10741 tgttcaagac aaaattgttt atccgccaga acggcagaag cataaggaga aaggattcgg
    10801 gtcgcggcaa ctttaaaatc ggtgagtaca ctgtcggcga tgggttttgg ctgccctgct
    10861 gccgcaaaca tcaggcgcat ggcctcacgc tgatcgacag ctaacatggc gaatccacca
    10921 cctgggcggg agatatcttt cagcgtaaat ggggtgaagt gggatgacat agaaactcct
    10981 gttaatagat aaaattaaag accagcctgc tggcgaacct gatttagcag ggcgctccag
    11041 tcctgccgcc ctttgccgtc ttgacttgct gcggcgtaat aactttctgc tgcaatcccc
    11101 atattgagcg gcgcccccgt tttccgagcg acgtcaactg caatacgcag atctttcaat
    11161 gccaggtcca ccatgaaggc gggagaaaga tcgcctttca gcactttgcc aggccaggtg
    11221 gtcgtaaaat gtcctttccc tgctgccgta ccactcatca ccttaattgc gacatcaaga
    11281 tttaatccca gtgactcaca taacacagcg gcttcggagg acagcgcatt aagcgcaatg
    11341 ctcatatagt tgttaattag cttcacgcgg atccccattc ctgggccgcc agcctcgacc
    11401 agctcattac ccatgcacat gagaatcggg cgtgcacgtg caatctgctc atcagtgcca
    11461 cccgctaaaa tcagcaatga gccatcaatc gcattcacgg acgttctgcc cacgggagcg
    11521 tccatcatac tgatgccttt ttcttgtaac tcccgtatta atgcatcggt ctgtaaggga
    11581 tgaatagtcg acatatcaat caataacgcg tcagatgaaa cagcctcaca gacgcctttt
    11641 tcccccaaca gaacctgtcg aacaatgtca ccattgggaa gcatagtgat gataaattca
    11701 gcgcctgctg ccgcttccgc tggggtttgt gccgcagtgg ccccctgcgt aactagcgca
    11761 tcgacggcct gtgcattgac atcaaatacc tgcaatgaat gccctttttg caatagattg
    11821 ttagccattg ggctacccat ctgccccagc ccgagaaatg caatggttgc catgaatact
    11881 ctcctgtctg acattgacaa aataatgaca attgaaaatt cgttttcgtc tgtttttgag
    11941 catatttgta atttatgttc aaaaacacga cacgcatcac taatttgatc atttgtgtca
    12001 tttaaattga acaaagacga caaaattaaa caggacaaga caaatatgac cagaattgca
    12061 tgcgtgggta tcacagttca ggacagaatt tattctttgc ccactctgcc ggagggcggc
    12121 gggaaatatc aggcaaatca ttatcttgag attggtggcg gcccggcagc aacagccgct
    12181 gttgcaatcg caaagcttgg tgtggaggtt gatttcatcg gtcgcgtcgg cgatgacagt
    12241 tgtggaaata ccttacttgc agaacttgag ggttggggcg tcaataccgc attttgccgc
    12301 cgatatccta acgcccgttc gtcgcaatca gcgatcctcg ttgatcagca tggcgaacgg
    12361 attatcgtta attatccaag tcctgatctc ggtactgatg ccgaatggtt agaagcgatc
    12421 gatttctctc gttatgatct gattctggcc gatgttcgct ggcatagcgg tactgaaaaa
    12481 gcattctcgc tagcgtgcct tgcgggtgtg acaacgcttt tagacgccga tatgacaccg
    12541 caggatatat ctccacttgt cgcgcttgcc gatcacgccg tgttctcaac accagggctg
    12601 aaacgcatga ctggcctgca gtcacctgaa gaaggtttgt tccaggcaac gacacaaaca
    12661 gcaggtaaag tgtatgttac gctgggttct gaaggatcgt tgtggattga agatggtcat
    12721 ttgtgtcaac aggaggcatt ttctgtcaat gtggttgata ccactggagc aggcgatgtt
    12781 tttcacggcg cactcgcggt agcgttggca gaaaaaatgc caacaaaaga ggctattcgt
    12841 tttgccagtg cggttgccgc gatgaaatgt acccagcctg gcggtcgggc cggcattcct
    12901 aatcgtgagc aaaccgaatc atttttgtca ctttatgcgt aaaatggtaa acatctaccg
    12961 aggaggggca agggagtatt catgagtatc atcgaagtga caggtaatcc acgccatgac
    13021 caactcgttc atctaatcgc agaacgcggt tatatgaata ttgaagaact ggcacaactg
    13081 ctggatgttt caacgcaaac cgtgcgccgg gatatccgaa aactcagtga gcagggcttg
    13141 atcacccgtc atcatggagg ggccgggcgc gtctccagcg tcatgaatac cgcttttgag
    13201 caacgggaac tttcgctcac cgccgagaaa cgggcgatcg ctgaggcagt cgccgattac
    13261 cttcccgaac gctgtaccgt ctttatcacc atcggtacaa ccgtagaagc cgttgccagg
    13321 gcattactca accggcgtga tttacgcatt atcactaaca gcctacgtgt ggcacagatc
    13381 ctttataaga atcaggatat tgaagtgatg gtgccgggag gcactttacg cgctcataac
    13441 ggcgggatta tcggcccagg agccgtggat tttattgaag gcttccgcgc agattattta
    13501 atcaccagta taggtgccat tgaacatgac ggcaccctgc tggaatttga tgtcaatgaa
    13561 gcgttagtcg cgagaacgat gattaaacat gcgcggaata cattgttagt ggccgatcat
    13621 acgaagtttg ccgcgtctgc tgccgtttca attggcaatg cacggaatgt cagggctttt
    13681 tttactgatg ccccgcctcc caattctttc tgccagttgt taagtgaaga gaatgttgaa
    13741 ctggtggttg ccgagcaaga agtatcctga aattgaaatt tcggttgata cggttttcgt
    13801 tggccggatt aagcacatcc ggccattaat caagcttcag ccgccagcat ctctttctta
    13861 cgttggctaa tacgcttaac cagcattacg gaaaccaccg caccacagaa tgcgaagcag
    13921 gccataatgc caaacacaat tttgtagcca ataatccccg gatttaaatc cagaatgtag
    13981 ccatacagac tgaagcagaa catcgccggg gcgtaaccaa taaagctacc caacgccatc
    14041 gccgcgcctg ttttattttc agcaattttt gcttcgccga taggtgcaaa aaatacggct
    14101 cgctgtgtaa agactatagc gccaaagccc agcgtacatg ccatccctaa ataaaccggc
    14161 atactttcgt gcggcagcat aatcaacagt acgagcgcag cggtactgat gataaaggtg
    14221 tagcatagat atttactcgg cgatttcagg atcttatctg aaatcatgcc accaatcggt
    14281 ccaccaatca ttttcagaca gtattggtta atgatgccgt aagcccccac cagcgcaacg
    14341 ggcaatagat agatgttttt caggaatgga atgaagaatg tcaggccaca gtaaaccgcg
    14401 taaacgaaga agacgttgaa agcgataagc cagatagttt tgtctttcag caccgaggtc
    14461 atactggtgt ttttcgatgc tccgtcttct tttttcacct caacggacgg tgcctcttct
    14521 ttgtcattca gaacaaagaa aatgataatg cctacggcaa tcacaataag ggaatagaac
    14581 cagatgcctg ctttaaaacc caataagcca ctgccaaacc aggtaaatac tgccaacgca
    14641 gaaaatgcca ccacggtatc gacaatgcca cgccctgttt cgaagaagcc aaacaaccga
    14701 ccttgttgtt cgctattgcc caatcgactt accgacttga gcaagaccgg ccagttcatc
    14761 atgtcgcaag taacgccaaa tagcgcccag acaaagagga tgccccaata ccccggcatt
    14821 gtcgtcaaat aaacacccag taatcctgtc gcaatgagtg agaatgacat ggtgtatctg
    14881 cgcggtagtt tatcggcaaa ataaatagac agaaaaaagc ccactgtggt gacaaatgag
    14941 tttaccgaca tagcattacc aatttgacca ttggtcaaat ggaaatattc ctgcatcggg
    15001 atataaaacg catctttcag agacggtaat ttataaattg taccgccaca caatgttaat
    15061 agactaaata acgcccattt ctttttcgtg agcataacat cccctaagcg atttttaaaa
    15121 tcactgtttt aaaggcaata agataccgac cattcatatg gaatggtttt attaacagcg
    15181 tatgagttaa aacttattag ttgttcagaa gatggtttgc ctcttgtttg atttgttgta
    15241 gtacattacg cacatgattt atctgattaa ttgcatcttc tttttcttta cgtaaccgcg
    15301 catccaataa atgattttct ggtaggggtg tttcactcat ctggcgataa gggatccacg
    15361 gattatcatc ttcgtcttca aagcggaacg ccggcgcata gtaatcaacc tcttcttcca
    15421 ggccatatgc cgtcacctgc ggctcatcat cacccaggca gataaggtgc gttaataacg
    15481 ctttgaacgg catatcaccc tgacctgaaa tacaggcttt atgacccagg ccaccctctt
    15541 ctttaacgat caaggcatct ttgatatgga cctgggtaat atgcggtgcc atcgttttta
    15601 aagcgtcgat gggatgctca tttgcattga tcatgttcgc aaaatcaaac agtaaggaaa
    15661 gagattccat ctcactttct ttgaccagcg acaccagctc atggcttttt aaatcttcat
    15721 gctgttcgat cgtaaaagtc aggccgctgt cctgatacgt ttcccgtaca taggcaatgt
    15781 cgttagcgat gatcgataat acgtcgcgca aattaccttc ataacgtgga taaaaacgta
    15841 cggacgatgc cccagttttc aacgcaatgg cgacggcttc gtcaatagat gccttatctg
    15901 aagcgctggt ttcaatatga atatcaaggt tcagacggcg ggctttatcc ccaaaggcag
    15961 agagttcttt atcgtccata ttaccaagag aaaaacgctc gccatccaga acatggattt
    16021 tgacgccacg tagattattt tcacttgcga tatcaagtaa atcagcaggc aacactcttt
    16081 ccagccgcat atttaaatga aaggcataag catgtaaata aagaggaaga gtgtcgacac
    16141 gttgtagaat ctttcttgcg ttatttattg tcaccataaa acacctacag attaatgatg
    16201 aggtctacgg cctcaaatcg ccaatattca atatctatct ggacaatatt gccttgctgg
    16261 tcggcgcgat gcttttcaat taagatcgct ggcatacccg cagaaccgcc aagatacttg
    16321 ctgatatcgc ccggcattct gacgggtttg aaaatcaatt tttttactac cgtctctttt
    16381 tggtagcact tttcccagac tgcagaaaat gagtggtttt caagttgttc aataaaaccc
    16441 ggagcaactt caggattaat atatgtttcg tgatagaaaa ctttatgtcc ttccagcgcc
    16501 ccccagccgg tgatgcgata gagttcagtt gatggtgtca ctgcaatcag tggcgcgagc
    16561 gtctcgggaa tatcgctggt acggtttttc tcggtaaacc cccaggaagg ctctcgtcct
    16621 tgctcaattg cggcccgctg aaagctcgct gacagctccg gactgtaatt aaatcgcggc
    16681 tgggtcacga accacccctt acgatcctta cggaagattt tggattctgc ctcgaggttc
    16741 aacaacgcct ggcgcagcgt catacgttta atgcccagca gttctcccag ctctctttct
    16801 gaagggagtt ttccacccgg cgtggtgatg ccatctttca accaccgatc cagtttctct
    16861 tttgcatttt ctacggtgga ttgattctcc gccattgttt aatccttttg gtttaaacca
    16921 gatgaggcat cttacgctta cgccaaaaaa gaaagcaatt aacggcgtat gactttgcag
    16981 ataattgtga caaagctctc tattgacgta atcacagact tataagggag atactcgcat
    17041 tggtgtgcgc tcacgacagg taaaaaaaaa acctgccagc gatggcaggt tttttatgac
    17101 tgaaaagcga aattaatcgt ctttcggtgc gcggttggcg cgacgacgat cgttttccgt
    17161 caggtgacgt ttacgaatac ggatagaggt cggagtcact tctaccagtt cgtcatcatc
    17221 gatgaactcc agagcttgtt ccagagtcat gcggataggc ggaaccagaa caacggcttc
    17281 gtcagtaccg gaagcacgca tgttggtcag tttcttaccg gtcaggcagt ttacagtcag
    17341 gtcgttagag cggctgtgaa taccgataat ctgaccttcg taaacttcag caccgtgacc
    17401 gaggaacagc ttaccgcgat cctgcagacc gaacagcgcg aacgctaccg ctttaccctg
    17461 accgttagag atcagtacac cgttctgacg ctgacccact tcgcccggac gtacgtcgtc
    17521 gtagtggctg aaggtggagt acagcagacc agtaccggaa gtcatggtca tgaactcaga
    17581 acggaagcca atcagaccac ggcttgggat cacgtagtcg agacgtacgc ggcctttacc
    17641 gtctggattc atgtttttca ggtcgccttt acgttcgccc agcgcctgca tcacagaacc
    17701 ctgatgctgt tcttcaacgt ccagcgtcac gttttcatac ggctcttgtt tacgaccgtc
    17761 gatttcacgg aagattactt tcggacggga taccgccagt tcgaaacctt cacgacgcat
    17821 gttttcgata agaacagaca ggtgcagttc gccacgacca gaaacgcgga acgcatcggc
    17881 gtcttcggtt tcttctacgc gcagcgcaac gttgtgtacc agttctttgt tcagacgatc
    17941 caggatctga cgagacgtta cgaacttacc ttctttaccg cagaacggcg aggtgttaac
    18001 gcagaagaac atagaaacgg tcggctcatc aacggagagt gccggcagcg cttcaacgtt
    18061 ttgcgtgtcg caaacggtgt cagaaatgtt cagttcgccc aggcccgtga tcgcaacgat
    18121 atcgccagct tcagccagat cggtttcgat acgttccaga ccgaggtggc ccagcacttt
    18181 accgactttc gcgttgcggg ttttgccttc gctatcgatg atagtaacct gctggttcgg
    18241 cttcacttta ccgcgcttga tgcggccaat gccgataacg ccaacatagc tgttgtaatc
    18301 gagctgagaa atctgcatct ggaacggacc gtcaaggtca acgtccggcg caggaacgtg
    18361 gtcaacaatc gcctgataca gcggggtcat gtcttccgcc atatcttcgt ggtccagacc
    18421 cgcgataccg ttcagcgcag aagcgtaaac gatcgggaag tccagctgct cgtcggtcgc
    18481 gtcgaggtta acgaacagat cgaatacctg atccacaacc caatcaggac gcgcgccagg
    18541 gcggtcaact ttgttgataa caacaatcgg cttcaggccg taagcaaacg cttttttggt
    18601 tacgaagcgc gtttgcggca tcgggccgtc aaatgcgtca accaccagca gcactgagtc
    18661 taccatggac attacacgtt caacttcacc accgaagtcg gcgtgccccg gggtatcaac
    18721 gatgttgata cggtaatcat tccatttgat agcggtgttt ttcgcgagga tggtaatccc
    18781 acgctctttc tccaaatcgt tggagtccat cacgcgctct tgggtttcgg cacgagagtc
    18841 gaacgtaccg gattgttgga gcagcttgtc taccagggtg gttttaccat ggtctacgtg
    18901 cgcgatgatg gcgatattac gcaatttttc gatcacaact ttgcctcagg cattagaaat
    18961 agcgcgttat tgtacacgga ttaatcgcac tacaaaacag gatcacaaac atcctccgca
    19021 aacaagtatt gcagagtccc tttgtgatcg ctttcacgga gcataaaaag ggttatccaa
    19081 aggtcattgc accaatatgg tgcttaatgt ttccattgaa gcactatatt ggtgcaacat
    19141 tcacatcatg gtgcagccct tttgcacgat gttaagcatg ataacgcctt ttaggggcaa
    19201 tttaaaagtt ggcacagttt tcgctttatc ttttttacgg cgacacggcc aaaataattg
    19261 cagatttcgt taccacgacg accatgacca atccaggaga gttaaagtat gtccgctgaa
    19321 cacgtactga cgatgctgaa cgagcacgaa gtgaagtttg ttgatttgcg cttcaccgac
    19381 accaaaggta aagaacagca cgtcactatc cctgctcatc aggtgaatgc tgaattcttc
    19441 gaagaaggca aaatgtttga cggctcctcg attggcggct ggaaaggcat taacgaatcc
    19501 gacatggtgc tgatgccaga cgcatccacc gcagtgattg acccgttctt cgccgattct
    19561 accctgatta tccgttgcga catccttgaa cctggcaccc tgcaaggcta tgaccgtgac
    19621 ccgcgctcta ttgcgaagcg cgccgaagat tacctgcgtt ccactggcat tgccgacacc
    19681 gtactgttcg ggccagaacc tgaattcttc ctgttcgatg acatccgttt cggatcatct
    19741 atctccggtt ctcacgttgc tatcgacgat atcgaaggcg catggaactc ctccacccaa
    19801 tacgaaggtg gtaacaaagg tcaccgtccg gcagtgaaag gcggttactt cccggttccg
    19861 ccggtagact ctgctcagga tattcgttct gaaatgtgtc tggtgatgga acagatgggt
    19921 ctggtggttg aagcccatca ccacgaagta gcgactgctg gtcagaacga agtggctacc
    19981 cgcttcaata ccatgaccaa aaaagctgac gaaattcaga tctacaaata tgttgtgcac
    20041 aacgtagcgc accgcttcgg taaaaccgcg acctttatgc caaaaccgat gttcggtgat
    20101 aacggctccg gtatgcactg ccatatgtcg ctggctaaaa atggcgttaa cctgttcgcg
    20161 ggcgacaaat atgcaggtct gtctgaacag gcgctgtact acattggcgg cgtcatcaaa
    20221 cacgcgaaag cgattaacgc cctggcaaac ccgaccacca actcttacaa gcgtctggtc
    20281 ccgggctacg aagcaccggt aatgctggct tactctgcgc gtaaccgttc tgcgtctatc
    20341 cgtattccgg tggtttcttc tccgaaagca cgtcgtatcg aagtccgttt cccggacccg
    20401 gcggctaacc cgtacctgtg ctttgctgcc ctgctgatgg ccggtcttga cggtatcaag
    20461 aacaagatcc atccgggcga agcgatggac aaaaacctgt atgacctgcc gccggaagaa
    20521 gcgaaagaga tcccacaggt tgctggttct ctggaagaag cactgaacga actggatctg
    20581 gaccgcgagt tcctgaaagc cggtggcgtg ttcactgatg aagcgatcga tgcgtacatc
    20641 gctctgcgtc gcgaagaaga tgaccgcgtg cgtatgactc cgcatccggt agagtttgag
    20701 ctgtactaca gcgtctaata gttgaagttg tactacccgg cgcaacaacg ccgggattta
    20761 gttgccgtgg aaactttcag cccatctctg catgggcttt tttctccgtc aattctctga
    20821 tgcttcgcgc tttttatccg taaaaagcta taatgcacta aaatggtgca acctgttcag
    20881 gagactgctt tatggcaaca ggcacgcagc ccgatgctgg gcagatcctc aactcgctga
    20941 ttaacagtat tttgttaatc gatgacaacc tggcgatcca ttacgccaac cccgccgcgc
    21001 aacaactgct cgcccaaagc tcccgcaaat tgtttggtac accgttaccg gaactgttga
    21061 gctacttctc attaaatatc gagctgatgc aagaaagtct ggaggcgggg caaggcttta
    21121 ccgataacga agtgacgctg gtcatcgacg ggcgctcgca tatcctttct gtgacggccc
    21181 agcgtatgcc ggacggcatg atcctgctgg agatggctcc gatggataac cagcgccgct
    21241 taagtcagga acagctacag cacgcccagc aggttgctgc ccgtgattta gtgcgcggcc
    21301 tggcacatga gattaaaaat ccgcttggcg gtttacgtgg cgcggcgcag ctgctcagca
    21361 aagcattacc tgacccgtca ctactcgaat ataccaaagt gattatcgaa caggcggacc
    21421 ggctgcgaaa tctggtcgac cgtctgttgg gaccgcaact gcccggtacg cgcgttaccg
    21481 aaagtattca taaagtggct gaacgcgtgg tgacgctggt gtcgatggaa ctgccggaca
    21541 acgtgcggtt gattcgtgat tacgacccca gcctgccgga actggcgcac gacccggatc
    21601 aaattgagca agtgctcctg aatattgtgc gcaatgcgct acaggcgctg gggccagagg
    21661 gtggtgaaat cattctgcgt acccgcacag ctttccaact gaccttacac ggcgagcgtt
    21721 atcggctggc ggcgcgaatt gatgtggaag ataacgggcc aggtattccg cctcatttgc
    21781 aggatacgct gttttacccg atggtcagcg gccgcgaagg tggcaccggg cttggcttat
    21841 ccatcgcccg taatttgatt gatcagcatt caggcaaaat tgaatttacc agttggccag
    21901 gtcataccga gttctcggtt tacctgccta tcaggaaata aaggtgacgt ttatgcaacg
    21961 agggatagtc tgggtagtcg atgacgatag ttccatccgt tgggtgcttg aacgtgcgct
    22021 cgctggagcg ggtttaacct gtacgacatt tgagaacggc gcggaggtac tggaggcgct
    22081 ggcgagcaaa acgccggatg tgctgctttc agatatccgt atgccgggaa tggacgggct
    22141 ggcgctgctc aagcagatta aacagcgcca tccgatgctt ccggtcatca ttatgaccgc
    22201 acattccgat ctggatgctg ccgtcagcgc ctatcaacaa ggggcatttg attatctgcc
    22261 caaaccgttt gatatcgacg aagccgtggc gctggttgag cgcgccatca gtcattacca
    22321 ggaacagcag cagccgcgta atgttcagct taacggccca acgaccgata tcatcggtga
    22381 agcgccagcc atgcaggacg tgttccggat tatcggtcgg ctttcgcgtt cttctattag
    22441 cgtgctgatt aacggcgaat ccggtaccgg taaagaactg gtcgctcatg ccctgcatcg
    22501 ccacagtccg cgagccaaag cgccgtttat cgcgctgaat atggcggcta tcccgaagga
    22561 tttgatcgaa tcagaactgt ttggtcacga gaaaggcgcg tttaccggcg cgaataccat
    22621 tcgtcagggg cgttttgaac aggccgatgg cggcacgtta tttctcgacg aaattggtga
    22681 tatgccgctg gatgtgcaga cgcgtttgct gcgcgtgctg gcagacggtc agttttatcg
    22741 cgttggcggc tatgcgccgg tgaaagtaga tgtgcggatt atcgctgcca ctcaccagaa
    22801 tcttgaacag cgggtgcagg aaggtaagtt ccgtgaggat ctgttccacc gcctgaacgt
    22861 catccgcgtt catctgccgc cgctgcgcga acgtcgggaa gatattcccc gtctggcgcg
    22921 ccatttttta caggttgccg cgcgcgaact gggcgtagaa gcgaagttgc tgcatccgga
    22981 aaccgaagcc gcgctgacgc gtctggcatg gccaggcaac gtgcgccagc tggaaaacac
    23041 ctgccgctgg ctaacggtaa tggctgccgg acaggaagtg ttgattcagg atttgcctgg
    23101 cgaactgttt gaatcaacgg ttgcggagag tacttcgcaa atgcaaccgg acagttgggc
    23161 gacgctgtta gcgcagtggg cagacagggc gctgcgttcc ggtcatcaaa atctgctttc
    23221 cgaagcgcag cctgagctgg agcggacgtt actgacgacc gcgttgcgac atacgcaagg
    23281 gcataaacag gaagcggcgc ggctactcgg ctgggggcgt aacactctga cgcggaagtt
    23341 aaaagagctg gggatggagt gattcacggc ttgtgtgtaa agattgatta ttgagcgcaa
    23401 attgctggta ttttgcgctt tactgttccg ataagttcag tatgatcttg cccggaaaac
    23461 ggggagagtc attatgctgg aatcaataat taatctggta tcgagtggcg cagttgacag
    23521 ccacacaccg caaactgctg ttgctgccgt gctgtgtgcc gcgatgattg ggctgtttag
    23581 ttgagtttca gattgctgat gaaatgctct ttgttcatgc cggatgcggc gtgaacgcct
    23641 tatccggtct acaaaagcaa tattttcaac aggttttgta ggcctgataa gcgtagcgca
    23701 tcaggcaatc ttgttctcag cacattgatg gcgctttcgt tttctacttt gtaaacgaag
    23761 cgccattcac tatttaaatc acccgtgaga actgctgcat ccgcgctttc tggcgcagat
    23821 aggtatcaaa gcacatgcaa atgttgcgga tcagcaagcg acctttcgcc gtcacctgaa
    23881 tccccttctc atccacatcc accagcccat cttttgctaa cggggcgagc agcttgagat
    23941 cttcggcaaa gtaatcagcg aagagcaaat cccactgttg ctcaatggga gcgtaatcca
    24001 gacggaagtt gcagatgagc gacttaatca catcgcggcg aatacagtca tcacgcgtta
    24061 gcgcaatacc acgccacagc gcattgcctt gttcatccac ttgctgatag tactgcttca
    24121 actctttctg gttctgcgcg tagcagtcgc caatcatgct gatggcggaa acgcccatcc
    24181 ccagcagatc ggtatcgccc tgagtggtgt agccctggaa gttacgatgc agcacgcctt
    24241 cacgctgggc caccgccagc tcgtcatccg gacgggcaaa gtgatccata ccgataaact
    24301 gatagcccga ttgtgtcagg aaggcgatgg tttcctgcag gatatcgagt ttttgctgcg
    24361 gactcggcag gtcagcatct ttgattttgc gctgagcagc aaaaatggtc ggcagatgcg
    24421 cgtagttaaa gacactcaga cgatcggggt tcagctccgc cacacgtttc agggtaaagg
    24481 cgaaactctc cggcgtctgt ttcggcaggc cgtaaatcag gtcgatgttg gtggaggtaa
    24541 agccaatctc acgcgcatgg ttaagcagtg caaagatgaa ctcttcatcc tgctcgcggt
    24601 taaccagacg ttgcacttct ttgttgaagt cctgcacgcc catgctcagg cgattaaagc
    24661 cttcggcgcg taaatgatcg agtacatcca gttcgatttc ccgcggatcg acttcgatcg
    24721 agatctccgc atcggcattg aactggaagt tttcgcgcag cagcttcatc agacggctga
    24781 tttgcgcttt attcagatac gtcggcgttc cgccgcccca gtgcaattgg ctgacgtgac
    24841 gcccggcgaa cagcggtgca cgatggacga tttcttgctc cagcgcgtcc agatactgat
    24901 cggccttgtg ctgctggcga gtaactatct tattgcaacc gcagaagtaa caaagcttat
    24961 ggcagaacgg aatatgtacg tagagagata atggacgctc aggatagcgc gccacggctt
    25021 gtaaaaacgc ctgttcgccg aagtcttctg aaaactccag cgcggtcggg tacgaggtgt
    25081 atcgtggccc ggaatagtta tatttctgga tcagggccag atcccagtcg atttgctgta
    25141 cagacatgct cactccttcc gatggcgtct ctggcggcta cggcgtaccg gcggatgccc
    25201 gtttcgagtc atcagccggt tgcgcagcca cttttgtcgc cgcgacaacc gctgtagttt
    25261 aacgaataac cacaccagat aacatataac cggaagggtt atcagcagaa cgggaaggcc
    25321 cacggcggca atccgttagt tgccccgcag cagacgcatc atgtcttctt gcttctcgtc
    25381 ttcttcctct tcttcgtcat cgtcataaga gaggccgagt ttctgcatca actcatcaat
    25441 acgatccagt ttggcatcca cccaggattg ctcttcggcg ctcagggttt cgcctgcctc
    25501 cagacgttcc agcagcgcat ccagacgctc atccgtttcc agtaactcca actccgcctg
    25561 cggtgaaagc ataggtttct cactcttcgg tttgtgctgt ttggtgactt tttcagtcac
    25621 gcccaatgga ataggggttt tactgccaat acgtggatct ttcggtgcgt tctggccttt
    25681 gctgcctgac gtggtgttac cgcccgctgc gcggctgccc ggcgcatgac cacgacgttt
    25741 tttctgacgc ttacggtcac gagcttcctg atccagctcc tcgcgtgttt ttcgacgcgc
    25801 ttttgcatga cctttgctgc gtgagtttga agatgatggt ttcataatgg ttcgtctttt
    25861 agccgtttta ttcagtatag atttgcggcg gaatctaaca gaaagcaagc aaagaaaaaa
    25921 ggcgacagat tactctgtcg ccttttttcc tgactcataa cccttaacgg gtcttacaat
    25981 ccttgcaggc cgccaacaca ccctgttttt ccattagccg gaacaatccg tgttgattcc
    26041 atttccgttt aattacgtct ccggacgttt gtcttcctga caatcctctg tcttcgcctc
    26101 ctggcgctcc tgaccctcat cctgagtcat tgttcctgtt agcgtcctcg ctctatgggt
    26161 gctactttac gcctttgctt taaacaaaca atcaaccgcc atcagtattt gcacattttc
    26221 agagttttat ataaaagcaa ttaactgatt tatatagctt atttgacatg catcgcagaa
    26281 ttttctctgc tatttcgcat aaagactatc ggcaatgtct cacaaagcgt atggctttta
    26341 cttacaacgc tcacgccgaa aaatccgcct tttattatgg ttcaggtata atcgccgcaa
    26401 agctttgact gacggagacg accgctttga ctaatttgaa ttatcaacag acgcattttg
    26461 tgatgagtgc gcctgatatt cgccacctac cttccgatac cggaattgaa gtggcttttg
    26521 caggccgttc caacgcaggt aaatccagcg cgctgaacac gctgactaac cagaaaagcc
    26581 tggcgcggac ctcaaaaacc ccagggcgca cccagcttat caacctgttt gaagtggctg
    26641 acggcaagcg tctggttgac ttgcctgggt acggttatgc ggaagtcccg gaagagatga
    26701 agcgcaaatg gcagcgcgcg ctcggcgagt acctcgaaaa acgtcagagc ctgcaaggtc
    26761 tggtggtgct aatggatatt cgccatccgc tgaaagattt ggatcaacag atgattgagt
    26821 gggcggtaga cagcaatatc gccgtgctgg tgctgctgac caaagcggac aaactggcaa
    26881 gcggcgcacg taaagcgcaa ttgaatatgg tgcgtgaagc ggtgctggcg tttaacggtg
    26941 atgtgcaggt tgaaacgttc tcttcactga agaaacaagg cgtggacaag ctgcggcaga
    27001 aactggatac ctggtttaac gagatgcagc ctgtagaaga aacgcaggac ggcgaataat
    27061 tttcttgcct taatgcttgt gccggatgtg gcgtatccgg cccgtaaatt caatagatta
    27121 cacttctacc gttggcctga taaacagggc gcatcaggca ttacggatct tttctttcgc
    27181 ccaataaaaa acgccccagt cattactgac tggggcggct aaaatattca gccaaatccg
    27241 attacgtgaa gtaaaaggtc tgaaagatag aacatcttac ctctgtaccc tacgcgacta
    27301 actttactct tttttgttca gttcagaaag cattttttgt aatttttttt cagttgttgc
    27361 ataggaattt ctaatagcca tcacaaaacg tgacagctta tgttgcttac ttacgaaaaa
    27421 agggcatgtt caggcgaatc ttagtgcgcc tgatcccagt tttcgccact ccccacttcc
    27481 accagcagcg gcacatccag acgggtacag ttttccatca gttgatgaat ctgcttcgcg
    27541 acggcatcga catcatcttt atgaacttca aataccagtt catcgtgtac ctgcatgatc
    27601 atacgcacac gcggttgctc agcctgtagc cacgcatcaa cggcaatcat cgcccgtttg
    27661 ataatgtcgg cggcggttcc ctgcattggc gcgttaatgg ctgcacgttc agccgctgca
    27721 cgacgagccc cattgctgga tttgatatcc ggcagataca gacggcgtcc gtccagcgtt
    27781 tcaacgtagc cctgctcttt cgcctgagca cgggtgcgtt ccatatactc cagcacgcca
    27841 gggtagcgtt cgaagtaaag gtccatgtac ttctgcgctt ctttacgtgg aatgttcaat
    27901 tgccgcgcca gaccgaaagc actcatgcca taaatcagac caaagttgat cgctttcgcg
    27961 ctacggcgtt gctcgctggt tacggtttcc agtggcaaac caaacacttc tgccgccgta
    28021 gcccggtgga tatcttttcc ttccgcgaat gcggtcagta agcctttgtc acgcgagaga
    28081 tgcgccataa tgcgcagttc aatctgcgag tagtccgccg agacaatcac ataatcctct
    28141 ggcgcaataa acgcctggcg gatacgacga ccttcttcgt tacgcaccgg aatgttttgc
    28201 aggttaggat cggttgacga taaacgtccc gttgcagtta ctgcctggtg ataagaggta
    28261 tgcacacgcc cggtttttgg gttgatcatc agcggcagct tgtcggtgta agtcgacttc
    28321 agcttcgcca gaccacgata ctccagaatc acttttggca acggatagtc cagcgccagt
    28381 tcttccagta cctcttccga cgttgacggc gcaccacccg gcgttttttt cagcggttta
    28441 atgctctgtt tttcaaacag aatggtttgc aactgcttgg tggaggaaag gttaaattct
    28501 tcacctgcaa tttcatgcgc tttcttttcc agctcagcca gacgaagagt gagctcttca
    28561 gaatgattgt gcagcacttt cggatcgatc ttcacaccgt tacgttcaat gcgtgaaagc
    28621 accggcacta gcggcatttc gatattctcg aagacgttca acggcccttt gtgtttttgc
    28681 agatccggcc acattttcag atgcaactgc aaggtgacat ctgcatcttc ggcggcgtaa
    28741 cgtccggcct cttcgagggc aatctggtta aaggtcagtt gatttttgcc tttacccgca
    28801 atctcttcaa aagtgatggt tttgtgcttc aaccaacgtt ccgcgaggct gtccatatcg
    28861 tgacgcccgg caacgctatt gagaatgtag gactccagca tggtatcaaa cgcaatccca
    28921 cgcagctcaa tgccgtaatt cgccagaata ccgcgatcgt atttcaggtt ttgcccaacc
    28981 ttcagcgcct tttcatcttc cagcagcggt tttagcaact cgagtgcacg ctcgcgagag
    29041 atttgatcgg gcgcatcaag ataatcatga gcaaccggaa tatatgccgc tacgcctggc
    29101 tcgatagcaa aagaaagccc gaccaggtta gcagagatgt tatcaaggct gtcggtttcg
    29161 gtatcaaatg caaataccgg cgctttttcc agcttcgcaa tccacgcttt cagtgtttct
    29221 tcatcaagga tggtgacgta gttgtcataa gaaatcaccg ttgccgtcac ttctggcgct
    29281 tcgtctgcaa cactggtttc ctgcagcctg gctgcgggtt tcgccccttt agcctgtaac
    29341 catttgcccg cttcgacatc agcagtccag cgtttgaact catacttttt gaacagcccc
    29401 aacaactctt ccgccgccgg ttgttgcact tcgagttgtt cgcaggtcag ctccagttca
    29461 acgtcggttt taatcgtcgc cagctggtat gagagataag caacttcttt gttttgctcg
    29521 agcttcgctg ccattgtttt cgcgccacgg aagctcaacc cagcaatttt ttctggctcg
    29581 gcatacagcg tatccagtcc gccaagacct tgcagcaatg cctgcgcggt tttttcaccg
    29641 acgcccggta cgccagggat gttatcggag gagtcaccca tcagcgccag gaagtcgatg
    29701 atcagttctg gcggcacgcc gtacttattc accacctctt ccgggccgag gatggtattc
    29761 gtcatggtat tgataagcgt aatatttggc gtcaccagct gcgccatatc tttatcgcca
    29821 gtgctgatca gcaccggacg cccggctttt tcggcttccc gcgccagagt accgataacg
    29881 tcgtccgctt ctacgccaga aaccgccagc agcggcagtc ccatcgcttt aaccatcgcg
    29941 tgcaagggtt cgatttgtgc acgcagatcg tccggcattg gcgggcgatg tgatttgtaa
    30001 tgttcaaaca gttcatcacg aaaggttttt cccttggcgt caaagaccac cgctgcatgc
    30061 gtcggtttat attgcatgat cagactgcgc agcatgttga ggacaccata catcgcaccg
    30121 gtcggctcgc ctgcgctgtt agtcagcggg ggaaacgcgt gatatgcgcg ataaagataa
    30181 gatgaaccat ctacaaggat aagtggattt tgggggatct gaaccataat gtccgtgcct
    30241 gtttatcaga ttatgggtaa aggatgccac agacaggatg aaaacatgag tttttcaccg
    30301 cttttcgtga aaatcttttg tagatcttct ggatcgctcg caaatcgtca tgtggataac
    30361 tttgtgcata aattatttac atcgctttta atttattatc atgcttttta cagcatgatg
    30421 tttgtctttg tttttcatga agttaaaaat aagaccatcc ctagtaatta agataatggg
    30481 attttttcta atgtggatag atatgaatta tttttctcct taaggatcat ccgttatttg
    30541 ggtcgttttc tgctaaaatc tgcgccttgc gcttctttag cgcactgtct atggctaacc
    30601 ttttgaataa attcattatg acgagaatac tcgctgcgat aacccttttg ctgagtattg
    30661 tattgaccat tttggtcact attttttgtt ccgtcccgat catcattgcc gggattgtaa
    30721 aactattgct acctgtgcca gtcatttggc gaaaggtttc gcgtttttgt gattttatga
    30781 tgtattgctg gtgtgaatgt ctggcggtat tactgcatct gaacccatac ctgcaatggg
    30841 aagttcacgg gctggaaggg ttaagtaaga agaactggta tctgcttatt tgtaaccacc
    30901 gtagttgggc agatattgtc gtactgtgcg tgctgtttcg taagcacatt ccgatgaata
    30961 aatatttcct caagcagcag ctggcctggg tgccttttct tggcctggcg tgctgggcgc
    31021 tggatatgcc gtttatgaag cgttattccc gcgcttattt attacgtcat ccggagcgac
    31081 gtggtaaaga tgtcgaaacc actcggcgtt cttgtgaaaa gtttcgcctg catcccacca
    31141 ccattgtcaa tttcgttgaa ggctcccgtt tcacgcaaga aaaacatcag caaacccact
    31201 ccacttttca aaacttgttg ccaccaaagg cggcaggcat tgcgatggcg ctaaatgtac
    31261 tgggtaaaca attcgataaa ctgcttaatg ttacgctgtg ttatccggac aataaccgtc
    31321 agccgttctt cgatatgtta agcggtaaac tgacgcggat tgtcgtacat gtggatttac
    31381 agcctattgc cgatgagtta catggcgact acatcaatga caaaagcttt aagcgccatt
    31441 ttcagcaatg gctgaactca ctgtggcagg aaaaagaccg gcttctgacg tcgttgatgt
    31501 catcacagcg gcaggacaag taaattttgc tcgcctctga atgagcagag gcgagtgagt
    31561 attttaatga acaaaacgtc cggcacgaga cataaattct tcttcgctca tctcctgtcc
    31621 gttaaaagca actttacccg gtgtataacg cagttgcagc gaggcggtat tgttgtcaat
    31681 cgtgattaac tgaaacatct gacctaaggt catcatcccg ctgatttgtt tatcagcctg
    31741 cttttgcgct ttttccgcat ccattccttc agataaatta agctgttttg cgatttctgt
    31801 ggcgacatta agcggtaatt tcacatcaaa attgagcgat ttaatatctt tgtttgtgga
    31861 tgatgaagat ttggctgggt cggcaatact gatatccaga ttggcattta gttcgccgag
    31921 tgcattcttc catcttaccg gttgtttaat ggtcggctca cttttttgta ataacggtaa
    31981 atactctttg aacaatgcag tattcacttc ttgcagagca acttcatcgt ttgctaactc
    32041 agggtgtgca gcaagctgct tctgcatcgc aatgttatac tggatgataa attggcgcac
    32101 cgctgaggga tcaatagatt cagcaatcaa tgaccactca ccactaccga gttgctgatt
    32161 cccttttgtt aacttatcaa gttcgtaggt cagattgaca ttgatataat ctttgtcttt
    32221 aacgcgatcc agcctggttt ttgcaacgaa agcatcgatt tgggcaacgt cttccccgtg
    32281 attgataatg ttaatctgat taaatttcga ctcgctttca ccaaccggaa atttcttttc
    32341 ttctaatcgc gccagagaat caatggtaaa acttttcgcc gttactttgt tcattggtga
    32401 taattgcaga acaatatctt cgactttgcc ttcaatatcc aggttagaca atgaactacc
    32461 atcaccatta aaagtaaact ggccctcacc aaaagaaaac tttccatatt ttgcaggggc
    32521 aacatctaat gtcgtggaga atgagccacc aaacgcgaat cgcaatgtcg cttccacggg
    32581 cgatttattt tttgcggcga taaatagcgg ttgtgtcagt tcattattca ctaatttcac
    32641 ttttgccgct gccagtgcgg ggatgacatt accacgcatt aacattgtga tgggcaacgg
    32701 accgtgctca acatccacat caaatgcaac tttctggcct ggcttgatat tgagatcggg
    32761 tgcaccgtta tcaaatgtta tttgcatttg aaaatgagaa ttaaaaaagc ctttgtcaaa
    32821 gtttttataa cttaacgtca tatcgtaggc atgttcacct ttctttttag catcgctaaa
    32881 atctttaata aacttttcga caccaggctg aatttgcgta cctgtgtacc atgtgccacc
    32941 accccagatt acggctaacg caacaataat acctgtagct gacttacgta tcatataaat
    33001 attccctgat aaaacagcaa aactttaaat atccgctta
//
LOCUS       DAPHCV010000052        32224 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.052, whole genome shotgun sequence.
ACCESSION   DAPHCV010000052 DAPHCV010000000
VERSION     DAPHCV010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.052"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(47..1444)
                     /gene="ccp"
                     /locus_tag="RH033_002393"
     CDS             complement(47..1444)
                     /gene="ccp"
                     /locus_tag="RH033_002393"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HDV2991569.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LANKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1849..3498
                     /locus_tag="RH033_002394"
     CDS             1849..3498
                     /locus_tag="RH033_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HDV2991570.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3549..4151)
                     /locus_tag="RH033_002395"
     CDS             complement(3549..4151)
                     /locus_tag="RH033_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDV2991571.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            4671..5570
                     /locus_tag="RH033_002396"
     CDS             4671..5570
                     /locus_tag="RH033_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2991572.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GIQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLNELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYQHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLASHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSRENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMTEHLD"
     gene            5619..6632
                     /gene="yhjD"
                     /locus_tag="RH033_002397"
     CDS             5619..6632
                     /gene="yhjD"
                     /locus_tag="RH033_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HDV2991573.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDAPRMPGK
                     TQP"
     gene            7025..8347
                     /locus_tag="RH033_002398"
     CDS             7025..8347
                     /locus_tag="RH033_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HDV2991574.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(8529..10589)
                     /locus_tag="RH033_002399"
     CDS             complement(8529..10589)
                     /locus_tag="RH033_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HDV2991575.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDTNAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(10659..11426)
                     /gene="pdeH"
                     /locus_tag="RH033_002400"
     CDS             complement(10659..11426)
                     /gene="pdeH"
                     /locus_tag="RH033_002400"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HDV2991576.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            11658..12587
                     /gene="kdgK"
                     /locus_tag="RH033_002401"
     CDS             11658..12587
                     /gene="kdgK"
                     /locus_tag="RH033_002401"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HDV2991577.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSQTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAEDAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(12683..14179)
                     /locus_tag="RH033_002402"
     CDS             complement(12683..14179)
                     /locus_tag="RH033_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDV2991578.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLEAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMTPSTAAAATSVATDDVHPEVTDIPPVQ"
     gene            complement(14400..15686)
                     /gene="dctA"
                     /locus_tag="RH033_002403"
     CDS             complement(14400..15686)
                     /gene="dctA"
                     /locus_tag="RH033_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HDV2991579.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(15869..17857)
                     /gene="hmsP"
                     /locus_tag="RH033_002404"
     CDS             complement(15869..17857)
                     /gene="hmsP"
                     /locus_tag="RH033_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HDV2991580.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQQRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(17939..21412)
                     /gene="bcsC"
                     /locus_tag="RH033_002405"
     CDS             complement(17939..21412)
                     /gene="bcsC"
                     /locus_tag="RH033_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
                     /protein_id="HDV2991581.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADTPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSETLGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSSNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALEKQGKWVQAAALQRQRLALAPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWSSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPLAKSQPPSMESAMVLRDGAKFEAQAGDPTQALETYKDAMVA
                     SGVTTTRPLDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTFEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDTWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDIDL
                     PPQPLIPYADW"
     gene            complement(21394..22500)
                     /gene="bcsZ"
                     /locus_tag="RH033_002406"
     CDS             complement(21394..22500)
                     /gene="bcsZ"
                     /locus_tag="RH033_002406"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HDV2991582.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNLLDWTQNNLAQGSLKEHLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWALLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(22507..24846)
                     /gene="bcsB"
                     /locus_tag="RH033_002407"
                     /pseudo
     CDS             complement(22507..24846)
                     /gene="bcsB"
                     /locus_tag="RH033_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
     gene            complement(24857..27469)
                     /gene="bcsA"
                     /locus_tag="RH033_002408"
     CDS             complement(24857..27469)
                     /gene="bcsA"
                     /locus_tag="RH033_002408"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HDV2991583.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPLDPVRYLIQTCWLLIGASRKETPKPRRR
                     AFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSLIL
                     ALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRYTS
                     TLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVDIF
                     VPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIARTTH
                     EHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHFFS
                     PDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIAVE
                     TVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLDNP
                     LTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLFHAYIIYAPALMIALFVLP
                     HMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGGLV
                     EEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIILGG
                     AVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQILEG
                     QKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWALWQ
                     DSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRPER
                     SETAQPSDQALAQQ"
     gene            complement(27466..28218)
                     /gene="bcsQ"
                     /locus_tag="RH033_002409"
     CDS             complement(27466..28218)
                     /gene="bcsQ"
                     /locus_tag="RH033_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HDV2991584.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICTGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEF
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            complement(28230..28418)
                     /locus_tag="RH033_002410"
     CDS             complement(28230..28418)
                     /locus_tag="RH033_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HDV2991585.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPDIDYADISQRER
                     LAAALKRWPLLAEFAQQK"
     gene            28691..30262
                     /gene="bcsE"
                     /locus_tag="RH033_002411"
     CDS             28691..30262
                     /gene="bcsE"
                     /locus_tag="RH033_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HDV2991586.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPGKITLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINSGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQKGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            30259..30450
                     /gene="bcsF"
                     /locus_tag="RH033_002412"
     CDS             30259..30450
                     /gene="bcsF"
                     /locus_tag="RH033_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HDV2991587.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            30447..32126
                     /gene="bcsG"
                     /locus_tag="RH033_002413"
     CDS             30447..32126
                     /gene="bcsG"
                     /locus_tag="RH033_002413"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HDV2991588.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLFVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDITEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFSEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
BASE COUNT         7611 a         8823 c         8068 g         7722 t
ORIGIN      
        1 cgtaataaaa atcgcccaaa cactcctgtt cgggcgatca aattaattat tgtttatcct
       61 gcatatacgg cgtgtaaacc ccgttcagac tgtgcaggaa agctacgata tcatccacat
      121 cctcctgcgg cagctctttg cctacctgat agcgcagcat cagtttcacc gccccgtcca
      181 gcgtcggcac gtcaccgcga tggaagtacg gtgccgttaa agcaacgtta cgtaaaccgg
      241 gtactttctg acgcaattta tcacgctctt ctttagtcac attcatacga ccaatatccg
      301 ccgccgtaat ttcaccaaag ttaaagtctt ttttcagccc caacggttca aaggaacgtc
      361 cgccgagaat aataccacca tgacaagttg cacatttatt atctttaaat aattgatagc
      421 cttttttctg ttgcgccgtc agagcattct catctccacg caaccattta tcaaatgggg
      481 aatccggcgt aattaatgtt ttctcaaatt cagcaatggc atcagtaata ttttcgccac
      541 tgaaaccttg cggatagact tcgaggaact gcgctttaag ctgcggatct ttttccagct
      601 tagcaataat ttcgtcccag gatttcgacg ccatttcaat cgggttcaac ggcggtccac
      661 cagcctgatc ctgcaatgtt gccgcacgac catcccagaa ctgctcaacg ttaaatactg
      721 agttaaatac cgtcggcgcg ttaatcggcc ccactgcgcc accaacacca atcgatgttt
      781 ttctgccatc gacgcccccc gcattcaacg catggcaatg agcgcatgaa atggtgctat
      841 cagccgataa acggggatcg tgatacagcg caaaacccaa cgccactttt tgcgcatcgg
      901 taggcagttt ttgcgggatg ggctgcaccg gttcattgcg atgctccgga gcagtatcat
      961 tgctggcgta atattccgcg cgctgttttg caatccaggc cagtatttcc gcccgctctt
     1021 catcactcac cttacccgcc cagtgtagcg cggtataacg cgttggcggc atagtttcat
     1081 actgcatcac ccattcaatc ttattcagat cgctttgcga aacgggttta ttagccagca
     1141 gagccgcacg cacggcctcg aggttaaaag atttatatcc aagctttatg tcgtaatcca
     1201 tcaactgttt cgcgccggga atataataat aggcgggtaa ttctgccgaa ggtgtatggc
     1261 aatagtcgca tcctttttca cggagaaagc ctaaaacctt attattttca ctgacagcag
     1321 atgcctgaac atcggcctgt ttactgcgtt tattatcgtg gtaccacaca taaccagata
     1381 accctaaata gcaaatcgcg acgccagcca ggccgatcgc ggtaatacgt gagaccattt
     1441 tcattattct tccctcacgg tggtgagtta ttgtgaccca gaaaattttc cagaatgctg
     1501 caaacgtctc tgcgagacaa agccatcata aaaaacaggg agatatgctt tttgatagga
     1561 atcaatcaat tacatgaata taaactatca ataagatagc cagtgcctat tacccagatc
     1621 aggaatggca tatcatgatt gcggcgagag caggattgaa tgttctgaaa atgaaaaaca
     1681 acacgaaatc cgtattttta taaattccat tgatattagt gcgttatatt ttttggcagg
     1741 ttaataaacg tgatccaccg cacgctttgt cgcccaccag gcggagcgaa tgactaccct
     1801 taaagaaaag cccgataatt agcgacgaat ttcggaggtc ggatccttat gctcaatcag
     1861 aaaattcaaa accctaatcc agacgaactg atgatcgagg tcgatctctg ctatgagctg
     1921 gacccgtatg aattaaaact ggatgagatg atcgaggcag aaccggaacc cgagatgatt
     1981 gaagggctgc ccgcctctga tgcgctgacg cctgccgatc gctatctcga actgttcgag
     2041 catgttcagt cggcgaaaat tttccccgac agtaaaacct ttcccgactg cgcacccaaa
     2101 atggacccgc tggatatttt aatccgctac cgtaaagtgc gccgtcatcg tgattttgac
     2161 ttgcgcaagt ttgttgaaaa ccacttctgg ctgccggagg tctactccag cgagtatgta
     2221 tcggacccgc aaaattccct gaaagagcat atcgaccagc tgtggccggt gctaacccgt
     2281 gaaccacagg atcacattcc gtggtcttct ctgctggcgc tgccgcagtc atatattgtc
     2341 ccgggcggcc gttttagcga aacctactat tgggattcct atttcaccat gctggggctg
     2401 gcggaaagtg gtcgggaaga tttgctgaaa tgcatggccg ataacttcgc ctggatgatc
     2461 gaaaactacg gccacatccc caacggcaac cgcacctatt atttgagccg ctcgcaacca
     2521 ccggtttttg cgctgatggt ggagttgttt gaagaagatg gtgtacgcgg cgcgcgccgc
     2581 tatctcgacc acctgaaaat ggaatatgcc ttctggatgg acggtgcaga atcgttgatc
     2641 cctaatcagg cctatcgcca tgttgtgcgg atgccggacg gctcgctgct caatcgttac
     2701 tgggacgatc gcgacacgcc gcgtgacgaa tcctggcttg aggacgttga aaccgcgaaa
     2761 cattctggtc gcccgcctaa cgaggtgtac cgcgatttac gtgcgggggc ggcctccggt
     2821 tgggattact cttcccgttg gctgcgtgat actggtcgtc tggcgagcat tcgtaccacc
     2881 cagttcatcc ccatcgatct gaatgccttc ctgtttaaac tggagagcgc catcgccaac
     2941 atctcggcgc tgaaaggcga gaaagagaca gaagcactgt tccgccagaa ggccagtgcc
     3001 cgtcgcgatg cggtaaaccg ttacctctgg gatgatgaaa acggcatcta ccgcgattac
     3061 gactggcgac gcgagcaact ggcgctgttt tccgctgccg ccattgtgcc gctctatgtc
     3121 ggtatggcga accatgaaca ggccgatcgt ctggcaaacg ccgtgcgcag ccggttactg
     3181 acacctggcg ggattctggc aagcgagtac gaaaccggtg aacagtggga taaacccaac
     3241 ggctgggcac cgttacaatg gatggcgatt cagggattta aaatgtatgg cgatgacctt
     3301 ctgggtgatg aaatcgcgcg cagctggctg aagacggtga atcagttcta tctggaacag
     3361 cacaaactga tcgagaaata ccatattgcc gatggtgttc cccgcgaagg cggcggtggc
     3421 gagtatccgt tgcaggatgg gtttggctgg actaacggtg tggtgcgccg tttaattggt
     3481 ttgtacggtg aaccataata tttttacagc cagccgctaa cttcctgctg gctgtaaaat
     3541 tatcctcttc aggaggagat atttaacatc attgccgcct gggtgcgatt tttcacttcc
     3601 agacggcgat acagggattc cagatgcgct tttaccgttc cggtactgat attcagcgcc
     3661 ctgccgatct ccttatttga ttcgcccgcc gctaacatgg ttaaaatctc ccgctggcgg
     3721 gcgcttaacg atttgagatc tttaatgtcc ttttccggcg tcgtccgcca gtctccaggc
     3781 agaaacatca tccccatcgc cgcactattt accgccaacg caaatgtctc gacggttgaa
     3841 tcacgaggca caatggccag cacattaaaa tggataactt cctgtaacca ccgtttattg
     3901 caatccgtcg ccgtaattaa caccttaacc tcaggaaatt gcaccacggt tttttgcagc
     3961 aaccagtaac aaaactcacc atcctgatcg ccatcgagca tcactaaggc ttcagggtaa
     4021 ctttccagct tttgccataa ctcgtctgcc tgactggccc cctgaatact cactcctgga
     4081 atacgttgct gtaaactgat tttcattcca tgaataaata ttgactgcct gtcaaacatg
     4141 actatttgca taactgaatc tccacctgaa tacgttaaaa agacttaagt agtggaaggg
     4201 tattacccgc gagaaaaaat aagaattcgc catttggcgg tggccattct acagagatga
     4261 cgcgtagaaa atagttaccg atataaatag ttacagctaa acgcctgaaa ttacatgtcg
     4321 agggcactat ttaaaacaat tttgaggatt tccttatatt agtggttagt acgcatgcaa
     4381 ttaaaaatga aattccgcga ccacaagcca aaataacaaa tggcaatgag acaaaaataa
     4441 gcacaaatag ccaatacgtc ctctattcac tttaaaggga atcgctgaaa aatacgctct
     4501 gtctaagggg attcaccttt ctaagaaagc tattcctccc ttttcctact gagaaatcgc
     4561 cacattcgtc atgacaacat tgtgaaaccc ggctttaggt gttagaaaaa acaataaaca
     4621 atgcgatatg cgcgttactg tttcgttgtg aacaacaatc ggtcaaagaa atggataaaa
     4681 ttcacgcaat gcagttgttc atcaaagtcg cggagctgga aagtttttct cgcgcggcgg
     4741 atttttttgc tttgccaaag ggaagtgttt cgcgccagat acaggcactg gaacatcaac
     4801 ttggcatcca gcttctccag cgcaccacgc ggcgagtcaa actcacgcca gaaggcatga
     4861 cctattatca acgggcaaaa gatgtgttga gtaatctcaa cgaactggac ggtctgtttc
     4921 aacaggatgc caccagtatc agcggtaaat tacgcatcga catcccgcca ggaatcgcga
     4981 aaagcctgtt actgccgcgt ctgtcggaat ttctctatca gcatccggga attgagctgg
     5041 agctgagtag ccatgaccgt ccggtagata ttcttcacga tggttttgat tgcgtgatac
     5101 gcactggcgc gttaccggaa gatggcgtta tcgcccgtcc cctcggcaaa ctgaccatgg
     5161 tcaactgtgc cagtccgcac tatctgacgc gctttggtta tccgcaaagc cccgacgatc
     5221 tggcttcaca cgcaatagtg cgttacacac cgcacctggg tgtacatccg ttaggttttg
     5281 aggttgccag cgttaatggc gtccagtggt ttaagtctgg cggcatgttg acggtaaaca
     5341 gtcgcgaaaa ctatctcgcc gccggtcttg ccggtctggg gattattcag atcccgcgca
     5401 ttgccgtgcg cgaagccctg cgtgccgggc ggcttattga agtattacct ggctaccgtg
     5461 ccgagccgct ctccctttcg ctggtttatc cgcagcgtcg ggagctttcc cggcgtgtaa
     5521 acctgtttat gcagtggctg gctggcgtaa tgacagagca cctggactga ccgactatac
     5581 tttttaagaa tgaccacgac agaaggacaa aagagcggat gacgcaggaa aacgagatca
     5641 aacgtcccac ccaggatctg gagcacgagc cgattaagca gttggataat agcgagaaag
     5701 gcggcaaagt tagccaggcg ctggaaaccg tcaccactac tgccgagaaa gtccaacgcc
     5761 aaccggttat tgcgcacctg attcgcgcaa cagagcgctt taacgatcgg ctgggtaacc
     5821 agtttggtgc ggctatcacc tatttctcat ttttgtcgat gatcccgatt ttgatggtgt
     5881 cgtttgccgc agggggcttt gtgctggcct cccatccgat gttgctacag gatatcttcg
     5941 acaaaattct gcaaaacatt agcgatccga cgctagccgc cacgttgaaa aacaccatca
     6001 acaccgccgt tcagcagcgt acgactgtag ggcttgtcgg tctggcagtg gcgctttatt
     6061 ccggcatcaa ctggatgggt aacctgcgtg aagcgattcg cgcccagtca cgcgatgtct
     6121 gggaacgctc gccgcaagat caggagaagt tctgggtaaa atatctgcgt gattttattt
     6181 cgctgattgg tttgttgatt gcgctgattg tgacgctttc tatcacctcg gttgccggtt
     6241 ctgcgcagca aatgattatt agcgcccttc acctgaacag cattgagtgg ctgaaaccga
     6301 catggcggtt aattggcctg gcgatttcca tcttcgccaa ctatctgctt ttcttctgga
     6361 tcttctggcg cttgccgcgc caccgtccgc gcaaaaaagc cctgattcgc ggaacatttc
     6421 tggctgcgat tggttttgaa gtgattaaaa tcgtgatgac ctacaccctg ccatcgctga
     6481 tgaaatcccc ctctggcgca gcatttggtt ccgtgctggg actgatggca tttttctact
     6541 tcttcgcccg tttgacgctg ttttgcgcgg cgtggattgc caccgccgaa tataaagacg
     6601 ccccgcgaat gccggggaaa acgcagcctt aaaataggcc ggatgcggcg tacgaattta
     6661 ttcggcctgg ctccccgtag gccggataag atgcgccagc atcgcatccg gctataatgc
     6721 gcacataacc tcttgaaact ctttcccaga gcctctttag ccatctattc aggagcaaac
     6781 aatttcattc caactcataa ccccagcata taaatccagt tggtaacttt tatttaacct
     6841 aaaaccagtt taatccacca tttataaaat tatgtgaagc atttcataga agaaaaatca
     6901 ctggcctaaa cattatcccc tttttgcatg atttttgacc atttccgcga tttgttacac
     6961 attgaaatat cacttttgct gtgcgtaata tggctattcg ttagccaaaa aataagaaaa
     7021 gattatgcaa gcaacagcca caacactcga ccacgagcaa gaatacacgc cgatcaactc
     7081 gcgtaataaa gtccttgtcg cctctctcat tggcacggcc attgagttct tcgactttta
     7141 catttacgcc actgcggccg ttattgtgtt tccgcatatc ttcttcccgc agggcgatcc
     7201 aacggcagca acgctacagt cgctcgccac cttcgccatc gccttcgtcg cgcgccccat
     7261 tggctctgcc gtttttggtc attttggcga tcgcgttggg cgtaaagcga cgctggtcgc
     7321 ctcgttgcta acgatgggga tttcgaccgt ggtgattggt ctgctgccag gctatgccac
     7381 gattggtatt ttcgccccgc tgctgctggc gctggctcga tttggtcagg gtctgggctt
     7441 aggtggtgaa tggggcggcg cggctctgct ggcgactgaa aacgccccac cgcgcaaacg
     7501 tgcattgtac ggctccttcc cgcagctggg cgcaccgatt ggcttcttct ttgccaacgg
     7561 cactttcttg ctgctttcct ggctactgac cgacgagcag tttatgagct ggggctggcg
     7621 tgtgccattt atcttctcgg cggtgctggt cattatcggc ctgtatgttc gcgtgtcgct
     7681 gcatgagtcg ccggtgtttg agaaagtcgc taaagcgaaa aaacaggtga agatcccgct
     7741 gggtacgctg ctgaccaaac atgttcgcgt aaccgtactg ggtacgttca ttatgctggc
     7801 aacctatacg ctgttttaca tcatgacggt ctactctatg acctttagta ccgccgccgc
     7861 gccagttggg cttggcctgc cgcgtaacga agtgttgtgg atgctgatga tggcagttat
     7921 tggttttggc gtgatggtgc ccgtcgccgg attactggct gatgcctttg gtcgccgtaa
     7981 aagcatggta atcatcacca cgctgatcat cctgttcgcg ctattcgcct ttaacccttt
     8041 gctcggttct ggcaacccga ttctggtttt tgccttcctg ctgctggggt taagtctgat
     8101 ggggctgacc ttcggtccga tgggcgcgct gttaccagag ctgttcccga cagaagtgcg
     8161 ttacaccgga gcatcgttct cttacaacgt atcgtcgatt ctcggggctt ccgttgcgcc
     8221 atatatcgca gcctggttgc agactaacta cgggttaggt gcggtggggc tatatctggc
     8281 ggcgatggct ggcttgacgt taatcgccct gctgctgacc catgagacgc gacatcagtc
     8341 gttgtaatca gagtgaaaag cctgatgcgc tacgcttatc aggccagaat ttccatacaa
     8401 tatattgaat tcgcaaggat ttgttggacg gataaggcgt tcacgtcgca tccggcatga
     8461 acaaagcaca ctttgccaac actctaaatc tgtttaattg ccggatgtca gacatccggc
     8521 aattatcatc acttcttcat ctgcgacaaa atcgtccgac actgattagc ctccccttcg
     8581 gaaggagaga tcagcgccag taacgccgcc gccggtgtta ccagcgttgc cagtgccgcc
     8641 gcaacagcac cacgggcaat caacggcccg gctttcaccc cagcctgcgg atttttaaac
     8701 gtcccacgca catacagcgg cgaacgcagt gtgataatcc gaatcccttt actttctgga
     8761 tcgatagtca aatccagttg ctccgaagca aaacttgccg taccggtaac attgatcaac
     8821 gcattctcag tatcaaaagc aaaaatctgc ggacgcgcca caccgttggc aatatcaaga
     8881 ttcgccgccg cgcagttcac ccgcacctca tcgtcaccaa atatcgcacc gacaatgtag
     8941 ttaccgacat tcagcccgac aatctccatc aggttgcggc tcaccagccc gtcattcatc
     9001 agcagtttca ggttgccgtt actgttgcct aaaagcgccg ccaccgagtt accgctaccg
     9061 cgtagttccg cgtcaccgtt catttccccc agcgtcttct gcatcagttc tacatcgggc
     9121 atcagttctt tcagtttcag tcgacgagcc tgaatatctg cccgcccttg catcggtttt
     9181 ttatcacctt ccagatgaat attcgccgca atgctgccgc ctgccatgcc aaatttcagc
     9241 ggttgcaggc gcaggtcagc atttttgagg atgatatgag tagaaagatc gctaatcggc
     9301 aggctactgc catgctcaat gcgccgccct ttgaagcgaa catcggcatc catgacgtcc
     9361 catttatcgg tttcgaagcg gtcataaggc agcactttgc ccgcaggctg aacgcttttt
     9421 tcacccttct tctgttcaga ccgtttcgac ttttcagctc ctttcccgga atcaacgcca
     9481 attaacggcc ctaaatctgc cagccgcaat tgccgcgact caacatcacc ttccagtttt
     9541 ggtcgtggct tgccggtggt gtagaccaga gaaccgtgga tatcgctatc gccaattcgc
     9601 ccattaaaac cgcgataatc aaagaccgac gatttttcag tgtcgatttt cgctaccagc
     9661 cgaccatccg tttcaaacgg cggggtatcg ggcagcagaa cgcccgtcag ttcatagaga
     9721 tcacccagcg aatcgccaga aaatttaagc cgtaaatcga caccacccat cttcattggg
     9781 tcattcacaa cgccatcaaa agcaacacgg gtgttaccag agcgaaaatc agcctgtaca
     9841 ggaaatggcg tcccttcgcc acgcagcgcc agcataccgc ctatttttcc cgtaccagtg
     9901 agcggctctc cgttataacg tccctgcgcc ttcaggccaa aaacgtaatc gcccaccttt
     9961 tctttatccg ctttaccttt cgatccagta acttcgctga acggcagcgg cttgcctaac
    10021 ggatcgacaa aaatttccag atccgctttg cttactttgt catcaatggc gatccgccct
    10081 tgatcgaaca gaatattatc cagccgaaac gaccatgccg acggctttgc attcgtgtct
    10141 ttgttgtcat cgttggcaag attaaacgtc cagttgttgt tcttttctga gaggcgaatc
    10201 aggcgtgcgt cgggcttttc gagcttgatc cacggcaacc agaccgtttt ggtcagcagc
    10261 gccagcgggg ccagcgtcgc ttctactcgc ggcaaatgca ccattgtgac ttcgggaata
    10321 tccggtggat tgccaagaat gatgtcttcc gcgtgtacat ggggccacgg cacccagctg
    10381 cgccagccag tttcttgttt ttgccgctcc cacaccacgc ccagatcgcc acggatagcg
    10441 aacggacgat tcaactccgc agagactttc tggttgatgg tcggtttgag tcgattccag
    10501 tcaaaagttg caatcaaaat gatcgccacg acaatcaaca acaagaaagc ccctgaaatc
    10561 gcagcggtta ttttgcctgc cttgctcatc gttcgcttcc tcatattctt cctgtgccag
    10621 tcctaaagat agtccagcca ggcggaaaat gaggcagctt atagcgccag aaccgccgta
    10681 ttcagcgttt ctatcggtgc cgggcgtgaa agaaaccagc cttgtgcggc gaatgcgggc
    10741 gagttctgaa catcacgcca ctcttctggt gtttctacgc cctcgacaat caccccgcga
    10801 caatagcgat tcattagatg taaaagctga gaaaagagtg tgcgtccttc cggtgactga
    10861 cgcagcatca caaacagttc tcgcgcgatt ttgatgtagt cataacgcac ttcacttagt
    10921 gcagagaaat ttgccatccc ggtaccaaaa tcatccagcc acagcgggcc aaattcacac
    10981 atcgaggcaa aggttgaatc tttcggcaga cggatatgct ccaccagttc gaaacgcagc
    11041 cagggaagac gctcaatctg gcgcaggatt tttggttgct gacgcagggc gatgagcgta
    11101 gggccgtcaa tattgaccga tgccaacagg ccgtgctcta caaagaagtc ggctttttgc
    11161 gccagcaaat caatctgctc tttcacaacc tccatccgat ggctaacggt gatttcagta
    11221 aaatagcgat ccggcggcag gcgttgcgaa gggttcaagg gatgcgtgac caccgttaat
    11281 agctccacgg ccattaaccg tccgcaggtt tgatagatcg gctgccaggt gtaagcacgc
    11341 tcacactgca accaaaaacg ccgttcctgc aagctctcga tgcttgcttc agggttgctt
    11401 attcgctgga taacctgcct tatcatcaaa gatgtcctgt gtttaaaagt gatgctgccc
    11461 ggactcgaca aagattatcg gcgcgcaagg gcagaacttt accgcctggt cggggtaaat
    11521 ttccccactc cacaacggaa tctttcagga atacgttctg gctcaaaaaa ataatggaac
    11581 actgttttaa tatggttgac cagcaaacca ccacagcgca aactaacgct aattttttac
    11641 agatcaggtt cacgactatg tccaaaaaga ttgccgtgat tggcgaatgc atgattgagc
    11701 tttccgagaa aggcgcggac gttaagcgcg gtttcggcgg cgataccctg aacacttccg
    11761 tctatatcgc ccgtcaggtc gatcctgcgg cattaaccgt tcattacgta acggcgctgg
    11821 gaacggacag ttttagccag cagatgctgg acgcctggca cggcgagaac gttgatactt
    11881 ccctgaccca acggatggaa aaccgtctgc cgggccttta ctacattgaa accgacagca
    11941 ccggcgaacg tacgttctac tactggcgta acgaagccgc cgccaaattc tggctggaga
    12001 gtgagcaatc cgcggcgatt tgcgaagagc tggcgaattt cgattatctc tacctgagcg
    12061 ggattagcct ggcgatctta agccagacca gccgcgaaaa gctgctttcc ctgctgcgcg
    12121 aatgccgcgc caacggcgga aaagtgattt tcgacaataa ctatcgtccg cgcctgtggg
    12181 ccagcaaaga agagacacag caggtgtacc aacaaatgct ggaatgcacg gatatcgcct
    12241 tcctgacgct ggacgatgaa gacgcgctgt ggggtcaaca gccggtggaa gacgtcattg
    12301 cgcgcaccca taacgcgggc gtgaaagaag tggtggtgaa acgcggggcg gattcttgcc
    12361 tggtgtccat tgctggcgaa gggttagtgg atgttccggc ggtaaaactg ccgaaagaaa
    12421 aagtgatcga taccaccgca gctggcgact ctttcagtgc cggttatctg gcggtacgtt
    12481 tgacgggcgg cagtgcggaa gacgcagcga aacgcgggca cctgaccgca agtaccgtta
    12541 ttcagtatcg cggcgcgatt atcccgcgtg aagcgatgcc agcgtaaaag cagcatatgc
    12601 aaaaggctgg atagagtgtt acccccctat ccagctcatc cataaatacc tgatggttgt
    12661 tctaactcat caccatcagg cattactgta caggtggaat atccgtcact tcaggatgta
    12721 cgtcatccgt ggcgacagat gtggcggcag ccgcagtcga tggggtcatg atttgatccc
    12781 aggccgcctg caacgctttc atgttaaatt ccggctcgcc tttcggctgc agcagtatta
    12841 acgccatatc attcgacaac tgctgacgca gatcctgatt taacatctcc accgtcaggc
    12901 tgttaaggaa atcctgacgc agtttctgat attgctccgg cgcgatatcg acgacctgat
    12961 tttgcaacga acgcatccgc tgccccatca gaatatcggt atcagctcgc gcataggcgg
    13021 caaacagttt ctgcagctcc agtttctttt gcgccactaa cgcattgaac tcttcttccg
    13081 gcagaccttt atcgcgaacc ttcgccagtt cacgcgccac cagattaagg ttgctgttca
    13141 gcttgtcgtt tggtgattcg atgttgatgg cacactgcgc acgcagatac agcacacgac
    13201 agtcaaatcc aagaccgata tctttgctgt tactggcgct taacgcttgc tgaacatgcc
    13261 agaacagcgc ctcacgggcc aggtccgcac gccagtagcg cagcagtgcg gctgattcac
    13321 gaatcggctg ccacggcgta tcccacatga ttgataaccg gtcctgacgc accgcgtcag
    13381 tcataatact caccgcttcc gcacgcagcg gagaaagcgt cggcaccgga gccggtgttt
    13441 cacgtttgcc tttcagttcg ccaaacgttt tattgatttg gtcgacgacc gagcgcgcat
    13501 ccacgtttcc caccaccagc agcgtcattg catccggggt ataccatttc tgatagaaat
    13561 ctttaatctt ttccgcttca acgggttgtt tcagcggatc ggcaggatcg tgacctaaca
    13621 aggttgatcc tttcagacga taacgccacc agccctcttt agtatcggca ggccaggttg
    13681 ccaccatgtc ctgactttgc agcgcgtggt tgatggtttc tggtgtgatg gtcagtttgc
    13741 cagtggcatt tgccagataa gagagcgctt ctttcagcaa gtcgttacgg ttattgggca
    13801 aactcagatt aaacagcgtg gtgtcataag agacaattac tggcggcatc gggcgtttag
    13861 ggtcgatccc ctgctgccac aatgaacgcg cctgtgctgc ttcaaggcca ccgctttgcg
    13921 ttaacgcaat acgagggatg gcgtggctgt aaccgctctg ttgtgtactt tcggcgagcg
    13981 aaccggtatt aaccagcagg cgaatttcaa cacgatcgct aggacgctgg ggcgtggtca
    14041 gcacttgcca ctgtaaaccg ttggaaagcg tcccctgttg ccatgctgga tcaggctgga
    14101 gcgcatctgc ctgcacatag ccagcagtgg ccatcatcag caaaccgccc gctaaaagtc
    14161 gaatttttgt gccctgcatg tgaacccctg atcaactatc ctggtaataa aaaaactgcc
    14221 cgttgtcggt cagtctaaat gacgttaaaa acacttcgtg ttagaccgca agaacatgaa
    14281 aatgtcacgg aagaagtgaa ataaacctga actcacccag gtggtaagtt caggcacagg
    14341 ggtcaattat gcgcaaacac ccgcactcgg ggaagggagt gcgggcataa gtgatgagat
    14401 taagaggata attcgtgcgt tttgccatcc ggcgcacgat tattcagcac atcgtccagt
    14461 tttttgtggt ccagttcttt cacccactta gcaacgacaa tggtcgctac gccgttaccg
    14521 accaggttag tcagcgcacg agcttctgac ataaagcggt cgataccaag gatcagcgcc
    14581 agacctgcta ccggcaaatg gcccaccgca gagagcgtcg ccgccagcac gataaagcca
    14641 ctgcccgtta ccccggcagc ccctttggaa gagagcaaca acacaattaa tagcgtaatt
    14701 tggtggacga tatccatctg actgttagtc gcctgggcga taaacaccgc cgccattgtc
    14761 aggtatatcg atgtgccatc aaggttaaac gagtagcctg tcgggatgac cagccccacc
    14821 accgatttcc ggcagccgag tttctccatc ttgtcgagca tacgcggcag cgccgactcg
    14881 gaagaagaag tgcccagtac aatcagcagc tcttcacgga tgtagcggat aaatttgaag
    14941 atactgaaac cagtcgcttt agcgattgaa cccaatacca gcaccacaaa caggatacag
    15001 gtgatgtaga aacagataat cagctgcccc agttgcacca gtgtgccgac gccgtattta
    15061 ccgatggtaa acgccattgc cccgaacgca ccaataggtg ccagacgcat gatcatattg
    15121 atgatgccga aaatgacctg cgagaaactt tcgatcacgt taaaaatcag ttggcctttg
    15181 ctgcccagac ggtggagcgc aaaaccaaac agtacggcaa acagcagtac ctgcagaatg
    15241 ttaccgctgg caaatgcgcc aatgacgctc gccgggatga catccataat gaaggcgaca
    15301 atgccctggt ctttcgcctg atcggcgtaa accgctaccg ctttcgcatc aagcgttgcc
    15361 ggatcgacgt tcattccggc accaggctgc acgacgttaa cgatgataag accaataatc
    15421 agcgcgatgg tactgacaat ttcaaagtaa agcagtgcga ctgcgccggt acgaccgacc
    15481 gccttcatgc tttccatgcc cgcaatgccc gttacgacgg tacaaaagat gacaggagcg
    15541 atgatcatct taatgagctt aacgaacccg tcgccaagcg gtttcatttg ctcgcctatt
    15601 tcaggataga aatggccaag gagaatacca atggctatcg ctgtcaggac ctgaaagtaa
    15661 aggcttttaa acagagaggt tttcataggg tgtcctttag taaaaccaca ggtcttgtaa
    15721 ggttatgggg tacctgcggc cttaaaataa cacccagaca acatcacaga aatgtacctg
    15781 gatcataatt gaaacaaaaa ggttaaaaag tttgagctgg ctcgcacaaa ccagcacttt
    15841 ttaaagtttt gtaatcagtt tggggtagct acttttcttc caggtaactc tcttcgaaga
    15901 tttcaatagg gagtgggcga gcaaaaagga agccctgggc aataccaacg cccgcttttg
    15961 ccagccagtc gcgttgtgct tcagtctcca cgccttcggc aatcatttgt aagttcaggc
    16021 tttgcgccaa catgataatt gcagcaatca tgctgctatc ttccggcaag ccttcaacaa
    16081 acattttgtc gattttcagt acgtcgattg gcaacgattt catatgctgc agctgacgca
    16141 gccccgcata gcccatgccg aaatcatcca gcgccacccg aactccggca ttgcgcagcg
    16201 gacggaggat tgccaccgca gcatgagggt cgtcaatacg tcggctttct gtcacttcca
    16261 gaatcagtgt tcccggctga atgcgatagc gggttaacag ttccagcata tccgccacca
    16321 tattcgggtg catcagttgc agcgctgaga ggtttaccga caagggcagc ataatgccgc
    16381 gctcttgcca ggcagcaagc agtcgacagg actcttccag cacccagtga ccaacggtaa
    16441 ccatcagccc acagcattca atgcgatcga ttaagccatc cggcagatcc caactgccat
    16501 ccggttgctg gatacgcagt aacacttccg cactgaccag tttaccgctg gtcatctcga
    16561 cctgtggctg taaccaaatc gcaaactgat gattttccag tgcattaagg atatcgctct
    16621 cttccgtcaa ccgctgttgg gcggcttcca tctgctgcgg atcaaagaac tgaatctgat
    16681 tcttgccttt atggcgagcg gtaaatgccg cagaaatagc acgactgtaa agctgttcgg
    16741 cggtgagatc gccgtagaac atcgccacgc caatgctaca gtgcggacgg agttgaatac
    16801 gttcaatcgg caggcgctcg ctcatgatag tgagcacttg ctgacctaaa gtgattgcgt
    16861 gccacggttc ctgtacacca ttggcaatga cagcaaagtc ataaccacta atctgcgcga
    16921 ggatcatacg tggcgacagt accgatttga gtttttccac cagcgtcagc agcagaattt
    16981 ctcgttgcgc ctctttcagc acgcccgcag tatcacgcag ggtttcacag gtgatgatca
    17041 tcagcgcggt ggtttgttta cgcgcgacaa cctgctccag catctccatc agcaaggctt
    17101 tgttcggcaa atccgacacc gggaagcgca tcgcgttttc gttctgttct tcataatggc
    17161 gctgcagcaa ttgctggttg aggttgtaac tgcgcaccag cataccgatt tcatcgtcct
    17221 gatgcagacg tggtaatgcc agttggtgac caacaagctc ctgggcaggg atggcgttaa
    17281 gttcgcgagc aatattgcgt aacggatgca aaatcaggcg gttaatgcac cagctaatcg
    17341 ccaccgtcaa tattaacgac aaaagtaagt aaatggtcac taacgttgag agggtgctca
    17401 tcacgaactt atacatgcgg aaggaatccg cctgtagcac cagataggca attggctgcg
    17461 gatttgccgg acgttcgagc gagtagacac ccagcgagat ttgaaccggt agctcaaaca
    17521 ggcgagtaac cattaccggc accggacgct ctggaataaa acttttacgc agcgcctgga
    17581 actggttagg cagcactaca tcggcacggc tgaccacgcc tgccggttta atgctggcaa
    17641 gaatagcttc cgcttcggga atatcgcctt tcaaaatagc tgaagataag ggttcgcgga
    17701 cagagcgagc aatgctttcc agttgcgtag ccgtgttata gcgattctgc tggaccagat
    17761 ggaacagcaa aacggtgcaa aaaataaaaa cgaacaccag gacaacggct gccaccattg
    17821 ccatctgctt gattgttaac gagcgactta cgcgcaaatt gtctcccccc ggaaatccca
    17881 gattgccgcc cgagtatacc cgatcgcagc ggcattaaga gaggcgctat ctgaaaactt
    17941 accagtcggc gtaaggtatc agcggctgcg gcggtaaatc tatgtcaccc tgccatccgg
    18001 cggcggaata acgtacgtag agcaggaaat ggctgggtgc gtaatctttc gcctgctgga
    18061 tatcaattgc cgtgccgaca aaccagttgg aagtaacacg tcgttcaagt aatgcccgcg
    18121 ccgtatagcc gaagccctga ctgctgccgc catcgttgga ttgccgcgca gcttcttcct
    18181 gccagtcggt cgggatcaga ttcatcagcg gataacgcgg catggttttg gtgcgtgaat
    18241 gcgaccacga gccagacgcc cccagctccc acgaccagtt ttccgtgcgc tcccgccaca
    18301 tcaccggtat ggcaaacgac aggtattcct gcggactgta gtaaccgccc tgaccgagtg
    18361 agtagccact caggtctttg tcgtaatgcc agatcatgtt gttcaggccg atggtgacgc
    18421 ggcgattgtt ctggttgatg accttatagt aatagcccgt catccagcgc acgcgccagt
    18481 tatcttcgac atttttacca gttaactgat caccgctaag cgatgcccag acgccgtttg
    18541 cttcaccttt atcgtaactc agacttagcc ccacgccgtc ggcacgcacg ccgccccatt
    18601 ttttcccggt attgctcggg gagtcttttt gcccaccaaa ggccagcaaa gaactggaga
    18661 tgggccgacg gtgggcgtta acggtgtaac ccagcggccc gatatcatcg ctgtaactga
    18721 tgccgccgac cacatccacc acgttgaagc ccatcggcgt ggtgccgata tcccagctcc
    18781 aggtgtcgtt gcgccaaccg accgccacgc tggcaccgga atccgactgg ctgcggttgc
    18841 cgctacaatc ctgtaatgta caggtgcccc agttgtcatc ccatttacca tcggcattag
    18901 tggagaaact gccgacgttc atattgacga aatcactgcg aaagaacatc cgcccgtcag
    18961 aatacggcgc atccacctgc aacatggtag tgtgcgcttt cagatcggag taaccaccgg
    19021 tgccgctcga accccagtaa tcatgctcaa aggtgacgtt aagatcctgc tggcgataga
    19081 ggtccgccgc atcgctgcgc acgccgcgtt tcagccagtc atctttctcg tcattgcggg
    19141 tcagtcgggt aaaggtgtcg ttatccagcg gacgcgtcgt ggtcacaccg gatgcaacca
    19201 tcgcgtcttt gtaggtttcc agcgcctgcg ttggatcgcc cgcctgcgct tcaaacttcg
    19261 caccatcacg cagtaccatc gcactttcca tcgacggcgg ctgagatttt gccagcggga
    19321 tcaacttatt aaacgtctgc tgcgccgctg cggtatcgcc aagctgcgcc tgcgccagcg
    19381 ccacgcgccg ctgagtgttc agagaggcgt tatcagtggc gggcagtttc gccagctggc
    19441 tacgtgccgc cgctttgtca ccggcagcaa tatccacttc cgtcagacca agaatggcat
    19501 cggcgttagt tggctcccgc gtcaggacat tctgatatgc agcgcgagcg gcggtgtaat
    19561 cacgtcgttg ttgcgcccag tcagccagcg tgaggtctat gcgcgtggaa ggtggttgct
    19621 ggcgcagcat cgcttccgct tcagcttctt tgccgctttc tcgcaggcga ttagcggttt
    19681 ccaacacctg atcgctttgc agtcgattaa ccagctcctg aatattgctg ctccactgcg
    19741 cgcgcggcag gctgttgata tgcgccagcg ccgctctgtc ctggtcatga ccagagagat
    19801 acagcccgta agcgtaaacc tgctccgggt cgttcggctt ctgctgcgcc agattacgca
    19861 ttaacgtatc ggcctggctg cgttgtccgg cctgccagag atcctgtgaa agtcggtaag
    19921 taatccatac actgccgggg gccagcgcca gtcgttgccg ctgaagtgct gccgcctgca
    19981 cccatttgcc ctgcttttcc agtgcctctg cctgctgtgc cagacggtcg ttttgcaggc
    20041 tgcgttcgat atcatcaatg ctgcgccgct gactggcaga gagcgaggcg ataaacgctt
    20101 cggctttttc tggcgactgc tggcggtaaa tattcgccag cccgcgcacg gcgttagtgt
    20161 tgctactgtc catacgcagc gtctgctggt aatagcgttc agcggcgggg taatctttgc
    20221 gtgccatcgc cacatcgccc agccccagca ccgcatagct gtcggtgtta tcgacattac
    20281 gtgcctgctg gaacaaccgt tccgcccggt caggattatt ggctttcagc gcagcatcgc
    20341 cctgctggat tgccagccag tagcggttta ctttcagcag actgttccat ttgtcgttgt
    20401 tgctgctgtg cggatccagg gcgagggctt tttccagatt cgccaccgca ttggcgcgat
    20461 cgcctttctg agaatacgcc tggcccagcg cccccagagt ctcactgtct ttcgggttcg
    20521 cccgcaccgc ctgttgtagt tcgggaatgg ctttacccgc cataccagag tccaccgccg
    20581 ctaaaccttg cgcacgagcg cggaaagcag ggtcggccag ctgtttttgc tgttctgcca
    20641 gttgcgattg tgcagccgcc acgctatcgc catcgctaaa gatcgagaga tattttttca
    20701 gcgccgacac gctggcatcg ctgacgggca tgtctttaat ctgcccgtac cagattttag
    20761 aggccccttc gcgcccggcg tttgatttcg ccatctgttc caggacagca aaaccttcgt
    20821 cacggcgatc gctactaaac agcaatagcg ccagattgtt ttgcaggccc gtattgcccg
    20881 gcgtatccgc attaatgcgt tttagctgat taatcgcttc gccacggcga gctggaattt
    20941 tcgccaccgt actccagtac tcgacggcaa tgtcaccttc cggcggcgca ccgttgaaca
    21001 gtttgttgta actcgccaca gcttcttctg catgaccggt cgtcgcctgc aatcttgcct
    21061 gttgcagtgc ctgacgacca tccggcgtgg aaagcaacat tgtggtccgc gacgatttat
    21121 acgcatttga actcggcgct aactgcgaca gccgatcgag ctgtttttgc gcgccatcaa
    21181 tatcgccctg acgtaacaga gaacggaaac gggcggcaac gacgtccggg ttattcggat
    21241 caataagttc cagacgatac aacgactgtt gcaccaggtc ttcacgatgg gtcgcttcgc
    21301 ctaaccgaac ttgctccagc aactgttgct gagcggttgg tgctgcctcg accatcggca
    21361 tgacggccag accgagggaa agcgtgaata tgtttagtgt gaatttgcgc attcctggcc
    21421 ccagtcaggt aataactcac cttttgtcga gaagcggaaa cggtgttgat cccagccttg
    21481 tccaaacagg gtcagcacat agttgtaata ggcatcgctg ccaggaaagt tatcggccac
    21541 gcgctggcgc tgaacggcct gcgcatcgcg gttttgtaaa aagggcagca tggcggcaga
    21601 aaaaccgacc ggtcctttac cctgcgcttt ccccgtagcc acatccactt tttccggcgg
    21661 ataaccgttt ttctcagtga atgtcgccat cggtttaaac cggttgagca tccgcgcttt
    21721 ttgcggatcg ctgtcaggca tcatgcctac ccacatgtaa acgcggatag cgtcgtagct
    21781 gctgatcaat gttttttcgg cttttagctg ccagcctttg tctttctcat agcgcaccca
    21841 gtctggcgaa aagcctttcg gggcggtttc cagcaataaa cgttgattgg tttcgcgcag
    21901 cgtggtccac ggcgcgccaa agcgggtgaa atactgcgcc agcgtcggcg gcaggtagct
    21961 ggggttaaaa cgccagctgt tatcctctgc aaatcccact ttgcccggta acaacatgga
    22021 gcccagccca ggcaccgtca ccacttcctc ccgcgcgata cgttttagca atgcgctgcc
    22081 gatgtcggta taacgctgct ctttccacaa acgccccgcc tccagcaacg cccaggccat
    22141 ccagacatca ccatcggagg ccgaattgct gtccagcact tcccacttac tgttctcttt
    22201 cttgccccac agccaggcgg gcaaatgttc ttttaaagaa ccctgtgcga gattgttctg
    22261 cgtccagtcg agaagattat cgaaagctgc acggtcgtta gccgccaggg caaagaacat
    22321 gccgtaactt tgcccttcgg aggtggtgat tttgcgcgcg tcgctggggt cgatgacgcg
    22381 cccttcctga ctgatgtaat cctttttaaa ctgctcccag gcaggccagg tacaggctgc
    22441 ctgaacacta aaggcagcca gcagcagcat cgtcacgagt ccactacgca acacattcat
    22501 cttcaattac tcgttatccg ggttaagacg acgacggctg ataatacgca gcagacgcca
    22561 cagtacccat gccagcagca cgacgctgat tgccgccagc accgccagca gaatcggatg
    22621 gtttgccagc gcataccata agcgttcgaa ccacggcaga tggccaacgt aatagacgtc
    22681 gccaacacgc aggctattga taccggactc gcggatcacc gcgaccgaac cgaacatggt
    22741 ggcgcgtttg ccgctatcgt tcaccgcatc gttaagcatt tcataaccgc gtgggctatc
    22801 tgccagcagc gcaatcacgc tacgctggtc gttatacggc gactggaagc caatcaccgc
    22861 tgccatcgca cctgaagagg tcagcgttga ctgagtttct gccgcgcgat cgctctcgtc
    22921 cggtacaatg ccggggaacg gggtctggcg catcggcgtt ttcacccagc tttcggtcgc
    22981 ctgcaccaac aggtcgatct gcttgtcgtc tttcagtttg tccgggatac caccgacgat
    23041 catgatgtcg gcatctttcc cctgaatagt gctgccatca tcggtcaccg tcaggttaat
    23101 cgccgggaag cccgtctgcg caccgataaa gccaacggta ttcagcaagg tttccatctg
    23161 tgcttcgtta ggcgctttcg gcatcacggt gatggtttgt gacagatccg ccatccggct
    23221 gaacgggaag cccgcgttag caaaggcgcg tagatccggc atcgggatga agtggtaata
    23281 cttcgagaag tcgatggtgg aatcatcacc aatcaccaca tgattctgca ccggctggaa
    23341 ggtaatgcag ttatccaccg aaccgcccgg catcgggttc atatactcaa agtcaaagcg
    23401 cagctggttg gtcgcgccca gtttcagcgc cggaatagag acatccgttt tgccatccag
    23461 caaaccttgt aataccggaa tacgcagcag caggcggttc gcctcctgtt tactgctcag
    23521 gttgaaggat tgcaggaact ggttattcag gctgatatcc atccgtgagc tgtctttcac
    23581 tggcggcatg gtgtagcgat aattaatatc catatcaatg ccggtactgc gcatcaggta
    23641 gagatccggc ggcaggttta gcgaaacgtt aatcgctgct ggctcaagac cgctggattg
    23701 taactgttct tcataggttt tcagttcacc aaaggtaacc ggacgatcgg tacgcaccca
    23761 gttcggcgca tcgtacggct tacgcggtag cagcggtttc acttcattca ctaccacgct
    23821 ttcaccacgg aacagaatgt taccctgagc gatacctttc gctgcctgca acaggtcttt
    23881 gtcgtcacga ccaaacacca ccagcagttt gacataagga ttctgcggat ggttgatcat
    23941 ttcaatcacc ggggctttta ccgccggatg atcgcgcagg aagtccgggc gtttgtcatt
    24001 ggtggcaaaa acgatggcat tgcgatccgg caattggtta tagagcaccg ggaagttctg
    24061 cccacgccag ccagaacgct aaccaaacca cgaggcgaca atggcagatg cttgttgcag
    24121 cccgacatcc ggcgcacccg caaagaccat cggcaaggtg ttagtacggt tatcacgcgg
    24181 gtcaaagaat ggcaccggga agtgtgacag gtcattcttc acattcaggg tctgataggt
    24241 cagatccagt ccactgctcc gcccaacatc cagccaaagc gtggtgctgg ccgggttttc
    24301 gcacacgtcc tgataatgac cgacaaactc cagccgcaca cggttgaagt cggtaataaa
    24361 cagtgggtta attggcattt gtgccagcgt ttttttaccc aactgttctt tggtcactgg
    24421 cagcacgccc atcagttcat cattgagata aacctttaac tgcgactgga caggcagtaa
    24481 cgatggcgat ggcgtgtatt cgagattgag catcgccttc gtcaccactt catcgctgcg
    24541 cataccaaac tcaatgctgc cgttcgggtt aatgccacgt agcaccatgc tgcccggcgg
    24601 cggcgcaatt tgcgcaaagg tcagcttcac atcacgcgaa ggaccgttct gcgccacgac
    24661 tggcgcatca gcgccctgca ctcccggcat cacttgcccc acctgcggat tttgttccgt
    24721 ctgggcggct acagctggct cagcattgat cagtggttgc gttgctggcg tcgcctgcgt
    24781 catgaaagag gggaacgcac tcatccccat agccactgca caaatccaga atagttttct
    24841 tttcatcgcg ttatcatcat tgttgagcca aagcctgatc cgatggttgt gccgtttcgc
    24901 tccgctccgg gcggcgcgga ataaacgata caacccagga aaccagagaa gtcagcacac
    24961 ggaatatgcc cttcaccgaa gaaggcgcaa actccgccag atggcggtag ccacggaagc
    25021 cgagcttcag aatatccagc agactttcca gcggcttatc ttccggatag ctgtcctgcc
    25081 agagcgccca tgtatccgca cgggcaaacg tacactgcac aaaatcgata tgttgctggg
    25141 tggtgagcgg cattaatttc aacccaactt cattacccat cacgcgcgcc acctgggtcg
    25201 ggaagacgta ttcctgctga ccgcgtttaa gcaacagatt cactttctgc ccttccagaa
    25261 tctgcgcctg accgttgatc ttgatcccca aaccaccgtc ggagaaatcc tgaacggtac
    25321 acgagaagag gtgaccatct tcgcgggcaa ttgctgcggg catcgtcatc tccacgcggt
    25381 gcgatcggcg tacctgtttg ctttctaccg atactgcgac tgcgccgccg agaataatca
    25441 ggttgtagaa tacccacacc atactgacga ccacggtgag catctcagtt ggcggaccat
    25501 agaagtagcg ccagatccca accgctacgc ccaccaggtt gagcagaaca aggaagatgt
    25561 agggccgcga gatcacccag tcgacgtact cttcttccac cagtccacct ttggcggtga
    25621 cgttaaattt gcctttgtgc gggttaatca gcgccaccag cgtcggtggt gcgatatacc
    25681 acgccagcac tgtttcgtag atttcactcc agaaagagtg gcgatatttg ccctggatct
    25741 tcgagttagt caggctggca tggatcatat gcggtagcac gaacagggcg atcatcaacg
    25801 ctggcgcgta gatgatgtag gcatgaaaca gcaggaacgc cagcggcgca gtcaggaaga
    25861 tcagccgtgg aatgcccgac aagaaatgga acatggcgtt gacatagcac agccgctgag
    25921 caaacttcag ccctttaccg gtgagtgggt tatcgagacg gaagatttgc accatcccgc
    25981 gcgcccagcg aatacgctga ccgatatgcg ccgagagact ttcggtcgcc agccccgctg
    26041 cctgcggaat acgcatatac gctgaggtgt agcctcgacg gtgcagacgc agagaagtgt
    26101 gcgcatcttc ggttaccgtt tcgacagcaa ttccgccaat ctcatccagc ggcttacggc
    26161 gaatcaccgc acatgaaccg cagaagaaag tggcatccca catatcattg ccgtcctgca
    26221 ccagaccata aaacagcgtg ccttcgttcg gcgttttacg aaaacgcccc aggttgcgtt
    26281 caaacggatc cggcgagaag aagtggtgcg gcgtctgcat catcgccagc tgtttctctt
    26341 tcaggaacca gcccatggtc atttgcagga acgatcgcgt tggtacgtgg tcgcagtcga
    26401 atatcgacac gaactcgcct ttggcatatt tcagcgcatt gttgatgttg cccgctttcg
    26461 catgttcatg agtggtacgg gcgatatatt tcacccccac gttttgcgca aactggcgaa
    26521 actcttccct gccgccgtca tcgaggatcc agatgttcag cttgtctttc ggccagtcga
    26581 tacccagcga agcgtaaatg gtatttttca ccacgttgag atcttcgttg taagtcggga
    26641 caaagatatc caccgacggc cacagcgaca tatctttcgg caatggcacc ggctgacgat
    26701 tcagaggcca tacgacctgg aaatagccga gcaccagcac aatccacgcg tacgtttcag
    26761 cgaagagcag aataagcccg cacaccaggc tgaccggatc gtcccagttt aacgtcgagg
    26821 tgtaacgcca ccagatataa cggcaagaaa cggtcagcga cagcacaatc agcatcagcg
    26881 ccgagaagcg ccccggcatc cgccgtacga tcagcgctac cccccacagc agcatcagga
    26941 agataaactg cgctagcggg ttaaacggct gagtaacgca gattaacgcc agaatcagcg
    27001 agaaggtgac gatgatacca aggatcaacc gccgcgcccc ggcactcaaa tgaccgagct
    27061 cttttttctc atcaagatgc tgtgttttat ggctaacgcg ctcaggcagc tcgttcatcc
    27121 attgatggta acgtccacga atattttgta gacctgaaaa tgcccgcctg cgcggtttcg
    27181 gcgtttcttt gcgcgatgca ccgatcagta accagcatgt ttgaataaga taacggaccg
    27241 ggtccagcgg acgcgaggcg ttgatatgcg gatacaggtt tttatgttct gcgcgaatac
    27301 gctgccagcg cgggtgctcc agcggaataa aaatccaggc caggatcatc cagaaacagc
    27361 cgagcgtcgc gctgaaagcc gacgcgccgt gacgacgata atcgcgataa cgcccaataa
    27421 gccgtgcgtt gaccggcggg ataagcaacc accgggtcag gatactcatg atgcactccc
    27481 gactggcgtt ttcagcccgg aatagttcaa caggcaccag ttcgccagcg tcagtatttc
    27541 ttcagccgcc agcgcatcac tgcgaaattc accgactggt tgcttagctg ccagacattc
    27601 agccatcgct tcatcacgat gaatgagcat cggcagcaat cggcgctggc tttgcaacca
    27661 aagctggtaa atatcgtcct gaacctggct gccaatacgg aagtcattaa tcaaaatatg
    27721 tgcaccatcc ggcagcgctt gctgatgcag tcggatatgg cagttggcat ccacattgac
    27781 gattgccagc gagtgatcgc acaaactcag cagctggtgg gttatctgcg aggcatcacg
    27841 cggtaagtca attaaaatcc actggtaacg cccgctggct tttagttgct gtaagccggt
    27901 gcaaatatcg ctcagccggg tttgccagtg ctgtggattt tcttgttctt caatggataa
    27961 ctgaccaaaa ggcagcaaat cgagctgcga ggtatagcgc aacccggcgt cacgccagtc
    28021 ctggccatcc agcatagctc tggcccaacc ctgacggtgg gtaaaatcaa cgttaaatga
    28081 caggcgcaac aagttgtccg ggcaggcatc gaccaccagg acattttctc ccaacatttg
    28141 taatgaccag gctaatgcgg cggtgatggt tgttgtcccc acgcctcccc gaaccccctg
    28201 caatcccagt acggccattc accaatcccc tacttttgtt gcgcaaactc tgccagcaac
    28261 ggccagcgtt ttaatgccgc ggccaaccgt tcgcgttggg aaatatcggc ataatcaata
    28321 tcaggcagtg aaaatgcctg ctttaacgcc aaaatatcat tctggaagat atagcctatc
    28381 gcgggatcag gcagagtatc tggttcattg ttattcattt ttcttgatcc ctatgaaata
    28441 accgtaccgt tcatgagaca attctaactg tgagcatttc gtcggtttat tgtttacggc
    28501 gagacgggaa tgtgctcact ttttgacttt ccgttacgca acagaaaaag ttaaaaacga
    28561 aaagactaaa atcacatgtc ttgtgacctg ctaaattgat tcacatgcta aatctgataa
    28621 gttttaattt caatggtagg tttatttctt agctttcgct agtaaactga taatcagaca
    28681 aaatgttgac atgagggaca ttgtggaccc tgtattctct atcggtatct catcattatg
    28741 ggatgagctg cgacatatgc cagcaggcgg cgtctggtgg tttaacgtcg atcgccatga
    28801 agatgctatc agtctggcga atcaaacaat tgcatcccag gctgaaaccg cacacgtcgc
    28861 ggtcattagc atggacagcg atccagcgaa aatctttcaa ttagatgatt ctcaagggcc
    28921 gggaaaaata acattatttt caatgctaaa tcatgaaaaa ggtctatact atttggcccg
    28981 tgatttgcag tgttctattg atccccataa ttaccttttt attcttgttt gcgcaaataa
    29041 cgcatggcaa aacattcctg ccgagcggct tcgctcatgg ttggataaaa tgaataaatg
    29101 gagccggcta aaccattgtt cgcttttggt aattaattcc ggaaataata acgataaaca
    29161 attttcattg ttacttgagg aataccgttc actttttggt cttgccagtt tgcgttttca
    29221 gggcgaccaa catttgctgg atattgcttt ctggtgcaac gaaaaagggg tcagcgcccg
    29281 tcagcagctt agcgttcagc aacaaaaggg tatctggaca ttagttcaaa gcgaagaggc
    29341 ggagatccaa ccacgcagcg acgaaaaacg cattctgagt aatgttgctg tactggaagg
    29401 tgcgccgccg ctatcggaac actggcaact gttcaacaat aacgaagttc tgttcaatga
    29461 agcccgtacc gctcaggcgg cgacggtggt cttttcttta cagcaaaatg cgcaaatcga
    29521 gccactggcc cgcagcattc atactctgcg tcgccagcgc ggtagtgcga tgaaaatcct
    29581 cgtacgggaa aataccgcta gcctgcgcgc caccgatgaa cgtttgttat tggcctgcgg
    29641 tgcaaatatg gttatcccat ggaatgcccc actctcccgc tgtctgacga tgatcgaaag
    29701 cgtgcaaggg cagaagttta gtcgctatgt gccggaagat atcactacct tgctgtcaat
    29761 gacccagccg ctcaaactgc gtggtttcca gaagtgggat gtgttctgta atgccgtcaa
    29821 caacatgatg aataaccctc tattacctgc ccacggtaaa ggcgttctgg ttgccctacg
    29881 tccggtaccg ggtatccgcg ttgagcaagc cctgacgcta tgtcgcccta atcgcaccgg
    29941 cgatatcatg accattggcg gtaatcggct ggtgctgttt ctctcattct gtcggattaa
    30001 cgatctggat accgcgttga atcatatttt cccattgcct actggcgaca ttttctcaaa
    30061 ccgtatggtc tggtttgaag atgatcaaat cagtgccgag ctggtgcaga tgcgcctgct
    30121 tgccccagaa caatggggca tgccgctgcc tttaacgcaa agttctaaac cggtcatcaa
    30181 tgccgagcac gatggtcgcc actggcgacg aataccagaa ccaatgcgac tgttagatga
    30241 tgctgtggag cgctcatcat gatgaccatc agcgatatca ttgaaattat tgtcgtttgc
    30301 gcactgatat ttttcccgct gggctatctg gcgcggcact ctttgcgacg cattcgcgac
    30361 accttacgtt tgttctttgc taaacctcgt tatgttaaac cggccgggac gttacgccgc
    30421 acggaaaaag ccagggcaac caaaaaatga ctcaatttac gcaaaatacc gccatgcctt
    30481 cttccctctg gcaatactgg cgcggccttt ccggctggaa cttctatttt ctggttaagt
    30541 tcggcctgtt gtgggcggga tatcttaact tccatccgct cctcaatttg gtctttgccg
    30601 cgtttctgct gatgcccatt ccgcgctaca gcctgcatcg cttgcgccac tggattgccc
    30661 tgccgatcgg ctttgctttg ttctggcatg acacctggtt gcctggcccg gaaagcataa
    30721 tgagccaggg ttcgcaggtg gcggggttca gtaccgatta tttaatcgac cttgtcacac
    30781 gctttattaa ctggcagatg attggggcca tttttgtttt attcgtggcc tggttattcc
    30841 tgtcacaatg gattcgcatt accgtttttg tggttgccat tctgctatgg ctgaacgtac
    30901 ttaccctggc gggaccaagt ttctccttgt ggccagccgg tcaaccgacg accactgtaa
    30961 caacgacggg tggtaacgca gcggcaaccg ttgcggcgac gggtggcgca ccggtagtgg
    31021 gtgatatgcc cgcacaaact gcaccgccaa caacggcgaa ccttaacgcc tggctgaata
    31081 atttctataa cgcggaggcg aaacgtaaat cgaccttccc gtcttcgctg cccgctgatg
    31141 ctcagccatt tgaactactg gtgattaata tctgttcgct ttcctggtcg gatattgaag
    31201 ccgccgggtt gatgtcgcat ccgctgtggt cgcatttcga tattgagttc aagaacttta
    31261 actccgccac ctcctacagt ggcccggcgg cgatccgttt actgcgcgcc agctgcgggc
    31321 agacttcgca cactaatctg tatcaaccgg caaataacga ctgctatctg tttgataacc
    31381 tgtcgaaact gggctttacc cagcacctga tgatgggaca taacggccag ttcggcggtt
    31441 ttttgaaaga agttcgcgaa aatggcggca tgcagaccga attgatggat caaacaaatc
    31501 tgccggttat tttgctgggc tttgatggtt cgccggttta tgacgatacc gccgtgctta
    31561 accgctggct ggacattacc gaaaaagata aaaacagccg tagtgccacg ttctacaaca
    31621 cgcttccact gcatgacggc aaccattatc cgggcgtcag caaaacagcg gattacaaag
    31681 cgcgggcgca gaaattcttt gatgaactgg acgccttctt tactgaactg gagaaatcgg
    31741 gtcgtaaagt gatggtggtc gtggtgccgg aacacggcgg cgcgctgaag ggcgacagaa
    31801 tgcaggtatc tggcctacgt gatatcccta gcccgtctat cacagacgtc cccgttgggg
    31861 tgaaattctt cggcatgaag gcaccacatc agggggcacc gattgtcatc gaccaaccga
    31921 gcagcttcct ggctatctcc gatctggtgg ttcgcgttct tgatggcaag attttctccg
    31981 aagacaatgt tgactggaaa aaactcacca gtgggttgcc acaaacagca ccggtctccg
    32041 agaactcaaa tgcagtagtt attcaatacc aggataaacc gtacgttcgc ctgaacggcg
    32101 gcgactgggt gccttacccg caataagaac gaaaaaggcc gcagagtttc acccctgcgg
    32161 cctgatccgg gcgcaaattg ccattacggc agcctgacgc ccgcatgaca cgttacttcc
    32221 gctt
//
LOCUS       DAPHCV010000053        31768 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.053, whole genome shotgun sequence.
ACCESSION   DAPHCV010000053 DAPHCV010000000
VERSION     DAPHCV010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.053"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            75..677
                     /locus_tag="RH033_002414"
     CDS             75..677
                     /locus_tag="RH033_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HDV2991589.1"
                     /translation="MNINYPAEYEIGDIVFTCIGATLFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(679..1200)
                     /gene="ruvC"
                     /locus_tag="RH033_002415"
     CDS             complement(679..1200)
                     /gene="ruvC"
                     /locus_tag="RH033_002415"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDV2991590.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1235..1975)
                     /locus_tag="RH033_002416"
     CDS             complement(1235..1975)
                     /locus_tag="RH033_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV2991591.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(2004..2456)
                     /gene="nudB"
                     /locus_tag="RH033_002417"
     CDS             complement(2004..2456)
                     /gene="nudB"
                     /locus_tag="RH033_002417"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HDV2991592.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2574..4346)
                     /gene="aspS"
                     /locus_tag="RH033_002418"
     CDS             complement(2574..4346)
                     /gene="aspS"
                     /locus_tag="RH033_002418"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDV2991593.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            4656..5222
                     /locus_tag="RH033_002419"
     CDS             4656..5222
                     /locus_tag="RH033_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDV2991594.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            5219..6037
                     /locus_tag="RH033_002420"
     CDS             5219..6037
                     /locus_tag="RH033_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDV2991595.1"
                     /translation="MIYIGLPQWSHPKWVRLGITNLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTAKNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            6090..6485
                     /locus_tag="RH033_002421"
     CDS             6090..6485
                     /locus_tag="RH033_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HDV2991596.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6526..7269
                     /gene="cmoA"
                     /locus_tag="RH033_002422"
     CDS             6526..7269
                     /gene="cmoA"
                     /locus_tag="RH033_002422"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HDV2991597.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7266..8237
                     /gene="cmoB"
                     /locus_tag="RH033_002423"
     CDS             7266..8237
                     /gene="cmoB"
                     /locus_tag="RH033_002423"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HDV2991598.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPGKTVEGYPAPKRAVLIARKP"
     gene            complement(8402..10831)
                     /gene="torZ"
                     /locus_tag="RH033_002424"
     CDS             complement(8402..10831)
                     /gene="torZ"
                     /locus_tag="RH033_002424"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HDV2991599.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPICSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGSADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAVQA"
     gene            complement(10856..11956)
                     /locus_tag="RH033_002425"
     CDS             complement(10856..11956)
                     /locus_tag="RH033_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HDV2991600.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKDGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGVLTDPALADRKPLWQYAEKLDDTYCAGCHAPIASDHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(12344..13090)
                     /gene="cutC"
                     /locus_tag="RH033_002426"
     CDS             complement(12344..13090)
                     /gene="cutC"
                     /locus_tag="RH033_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDV2991601.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(13104..13670)
                     /locus_tag="RH033_002427"
     CDS             complement(13104..13670)
                     /locus_tag="RH033_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDV2991602.1"
                     /translation="MANWQSINELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            13886..15619
                     /gene="argS"
                     /locus_tag="RH033_002428"
     CDS             13886..15619
                     /gene="argS"
                     /locus_tag="RH033_002428"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDV2991603.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(15672..16064)
                     /gene="flhe"
                     /locus_tag="RH033_002429"
     CDS             complement(15672..16064)
                     /gene="flhe"
                     /locus_tag="RH033_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HDV2991604.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGVTLNHRGESMSSAPLS
                     TRQPAAGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(16064..18142)
                     /gene="flhA"
                     /locus_tag="RH033_002430"
     CDS             complement(16064..18142)
                     /gene="flhA"
                     /locus_tag="RH033_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HDV2991605.1"
                     /translation="MSNLAAMLRLPANLKSTHWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(18135..19283)
                     /gene="flhB"
                     /locus_tag="RH033_002431"
     CDS             complement(18135..19283)
                     /gene="flhB"
                     /locus_tag="RH033_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HDV2991606.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLTRRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKSILVGSVTG
                     FYLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(19485..20129)
                     /gene="cheZ"
                     /locus_tag="RH033_002432"
     CDS             complement(19485..20129)
                     /gene="cheZ"
                     /locus_tag="RH033_002432"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HDV2991607.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(20140..20529)
                     /gene="cheY"
                     /locus_tag="RH033_002433"
     CDS             complement(20140..20529)
                     /gene="cheY"
                     /locus_tag="RH033_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HDV2991608.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSAMPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(20544..21593)
                     /gene="cheB"
                     /locus_tag="RH033_002434"
     CDS             complement(20544..21593)
                     /gene="cheB"
                     /locus_tag="RH033_002434"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HDV2991609.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLTAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(21596..22456)
                     /gene="cheR"
                     /locus_tag="RH033_002435"
     CDS             complement(21596..22456)
                     /gene="cheR"
                     /locus_tag="RH033_002435"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HDV2991610.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(22475..24076)
                     /gene="tap"
                     /locus_tag="RH033_002436"
     CDS             complement(22475..24076)
                     /gene="tap"
                     /locus_tag="RH033_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HDV2991611.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQESQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYHISALVFISMIIVAALYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQWRGIEQVAQAVSQMDQVTQQNASLVEEAAAATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLHIAPVVS"
     gene            complement(24122..25783)
                     /gene="tar"
                     /locus_tag="RH033_002437"
     CDS             complement(24122..25783)
                     /gene="tar"
                     /locus_tag="RH033_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HDV2991612.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(25928..26431)
                     /gene="cheW"
                     /locus_tag="RH033_002438"
     CDS             complement(25928..26431)
                     /gene="cheW"
                     /locus_tag="RH033_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HDV2991613.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(26452..28416)
                     /gene="cheA"
                     /locus_tag="RH033_002439"
     CDS             complement(26452..28416)
                     /gene="cheA"
                     /locus_tag="RH033_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HDV2991614.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRDQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISPPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(28421..29347)
                     /gene="motB"
                     /locus_tag="RH033_002440"
     CDS             complement(28421..29347)
                     /gene="motB"
                     /locus_tag="RH033_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HDV2991615.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLCGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLNGGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEEPEVAPQVSVPTMPSAEPR"
     gene            complement(29344..30231)
                     /gene="motA"
                     /locus_tag="RH033_002441"
     CDS             complement(29344..30231)
                     /gene="motA"
                     /locus_tag="RH033_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HDV2991616.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLEFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(30358..30936)
                     /gene="flhC"
                     /locus_tag="RH033_002442"
     CDS             complement(30358..30936)
                     /gene="flhC"
                     /locus_tag="RH033_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HDV2991617.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(30939..31289)
                     /gene="flhD"
                     /locus_tag="RH033_002443"
     CDS             complement(30939..31289)
                     /gene="flhD"
                     /locus_tag="RH033_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HDV2991618.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         7461 a         8665 c         7769 g         7873 t
ORIGIN      
        1 acatcgcgac ccctgaaaaa ataaattttt tatgctatta cgtatattca tatctatttc
       61 aatggaatga caacgtgaat attaattatc ctgctgaata tgaaattggt gatatcgtct
      121 ttacatgtat aggtgctacc ttatttggtc aaatatcagc tgcatcaaat tgctggagta
      181 atcacgtcgg gatcattatc ggtcataacg gtgaagactt cctggttgca gaaagccggg
      241 ttcccctttc aaccatcacc acgctatctc gttttattaa acgttcgtct aatcaacgct
      301 atgctataaa gcgattagac gctgggctga cagaacaaca aaaacaacga attgttgaac
      361 aggttccctc ccggctacgc aaactttacc acaccggttt taaatacgaa tcttcgcgcc
      421 agttctgttc aaaatttgtt tttgatattt ataaagaagc gctatgtatt ccggtgggtg
      481 aaatagagac gtttggagaa ttgttaaata gcaatccgaa tgcaaaactc actttctgga
      541 aattctggtt cttaggttct attccgtggg agcgtaaaac cgtcacgcca gccagtttgt
      601 ggcatcatcc gggtttggtg ttgattcatg cggagggagt ggaaacgcct cagccggaac
      661 tgaccgaggc ggtataactt aacgcagtcg ccctctcgcc aggttcagcc gcgattcgct
      721 catctgcatc gcattctgac tgacgtgaca atgggtaatc gcgatagcta atgcatcggc
      781 agcatccgcc tgtggattag cgggcagttt cagcaaggtg cggaccatat gctgcacctg
      841 gcttttttcg gcactaccaa tacctaccac cgtttgcttt acctgacgtg ccgcatattc
      901 aaataccggc aattcctgat tcaccgctgc cacaatcgcc acgccgcgcg cctgacccag
      961 tttcagggct gagtcagcgt tcttcgccat aaagacttgt tcaatggcga aataatcagg
     1021 ctggaactgg gtgatgattt ccgtcacgcc cgcatagatg agcttcagac gagacggtaa
     1081 atcatccact ttggtgcgta tgcatccgct acccaggtag gacagttgcc tgcctacctg
     1141 gcggatgacg ccatagccgg tcacgcgcga acccgggtca atgccgagaa taatagccat
     1201 cacgcgtctc cgttttgctg tttagcaggc ctcatcagag agtcgctgca acctcatcag
     1261 agatttcacc gttatggtaa acttcctgca cgtcgtcgca atcttccagc atatcgatca
     1321 gacgcatcag tttcggtgcg gtttctgcat ccatatcagc tttggtggac gggatcatgg
     1381 aaacttccgc gctgtctgct ttcagacctg ccgcttccag agcgtcgcgt actttgccca
     1441 tttcttccca tgcagtgtag acatcaatag cgccgtcatc ataggtcaca acgtcttcag
     1501 caccggcttc caaggctgct tccatgatgg tgtcttcatc gcctttctcg aaggagatca
     1561 cgcctttttt gctgaacaga taggctactg aaccatcagt accgaggtta ccgccacatt
     1621 tactgaatgc atgacgcact tcagcaacgg tacggttgcg gttgtcagac agacattcaa
     1681 tcatgattgc agtgccgcca ggaccgtaac cttcgtagat gatggtttcc atgtttgcat
     1741 catcatcacc gcccacacct cgtgcaattg cgcggttcag agtgtcacgg gtcatgttgt
     1801 tagacagtgc tttatcaatt gctgcacgca aacgcgggtt agcgtccgga tcaccaccgc
     1861 ccagcttagc cgcggttacc agctcacgaa tgattttagt gaagatttta ccgcgcttag
     1921 catcctgcgc agctttacga tgtctggtgt tggcccattt actatgacct gccataaaaa
     1981 tatctccaga tagccctgcc tgttcaggca gcgttaatta caaactgttc aatcgcctgc
     2041 cggttgctcc aggacttagt gagcgccgcc gcagcaggcg catcaagcca cttgtaggcc
     2101 agatgttcag tgaagacgac ctggcgttcg tgcgggagcg caagacagaa ccatgattcc
     2161 gtattacgcg tcacgcccgg cgcatagcga tgacgtaaat gtgaaaaaat ttcaaattct
     2221 accgtgcgct gacagtcaat taaggtcagt tgttcagcga caacatcgat ggtgacctct
     2281 tcctttactt cgcgcatggc agcttgcggc gcggtttcac cctcttccac gctgccggtt
     2341 accgactgcc agaaatcggg atcgtcacgc cgctgcaaca tcagcacccg tttcgtatct
     2401 tgtgcataga tgaccactaa gatcgaaacg ggacgcttat acactttatc cttcacgctg
     2461 cctcttcgtt gactgccttc gctcatccca ttcacatagt tatctatgct catgggagtt
     2521 cactcagttg ccgcctcgat gcaacgcgaa tgatttcgtg tatttgagtc atatcagtta
     2581 ttctctgcct tcttcacaac ctgaatgctc agctcagcca gtgcagtcgg gttagcaaag
     2641 ctcggtgctt cagtcatcag acatgctgcc gccgtggttt tcgggaaggc gataacgtca
     2701 cggatattgt cggtgccggt cagcagcatg gtcagacggt caagaccgaa tgccagacct
     2761 gcgtgcggcg gagtaccgta tttcagggcg tcgagcagga agccgaattt ctcgcgctgt
     2821 tcctcttcgt tgatacccag aataccaaac accgtctgct gcatatcacc attatggata
     2881 cgcacagaac caccgcccac ttcgtaacca ttgatgacca tatcgtaagc gttcgccacc
     2941 gcattttctg gtgcagcttt cagttctgca gcagtcatgt ctttcggtga ggtgaacgga
     3001 tggtgcattg ccgtcaggcc gccttcaccg tcgtcttcaa acatcgggaa gtcgataacc
     3061 cacagcggtg cccatttgct ttcgtcggtc agaccaaggt ctttacccac tttcaggcgc
     3121 agtgcgccca tcgcgtcggc aacaattttc ttgttgtcgg caccgaagaa aatcatatcg
     3181 ccatcttgcg cggcagtacg atccaggatg gcttcgatga tttctgcatt aaggaacttc
     3241 gctaccgggc tgttgatacc ttccagacct ttcgcgcgtt cgttaacttt gatgtaagcc
     3301 agacctttcg cgccgtagat tttaacgaag ttaccgtatt cgtcgatctg cttacgggtc
     3361 aacgatgcgc cgcccggaac acgcagagcg gcaacacggc ctttcggatc gttcgccgga
     3421 cctgcaaata ctgcaaactc aacagatttc agcagatcgg caacgtcggt cagttccatc
     3481 gggttacgca gatccggttt atcagaacca taacggcgtt ctgcttctgc aaaggtcatt
     3541 accgggaaat cgcccagatc cacgcccttc acttccagcc acagatgacg caccagcgct
     3601 tccatcactt cacgcacttg cggcgcggtc atgaaagaag tttccacatc gatctgagta
     3661 aattcaggct gacggtcagc acgcaggtct tcgtcacgga agcatttaac gatctgatag
     3721 tagcggtcaa agccggacat catcagtagc tgtttgaaca actgcgggga ttgcggcagc
     3781 gcgtagaatt tacctttgtg cacacgagaa ggcaccaggt agtcacgcgc gccttccggc
     3841 gtggctttgg tcagcatcgg agtttcgatg tcgaggaagc cgtggtcatc cataaaacgg
     3901 cgcaccaggc tggtgatttt agcgcgggtt ttcaggcgct gagccatttc cgggcgacgc
     3961 aggtcgaggt agcggtattt cagacgcgct tcttcggtgt tgacgtggtt agagtcaagc
     4021 ggcagaacat ctgcacgatt gatgatagtc agcgaggacg ccagtacttc gatttcgcca
     4081 gtcgccatat cgcggttaat atttttttcg tcacgcgcac gtacggtgcc cgtgacctga
     4141 atgcagaact cattacgcag ttcagaggcc agctttaacg cgtccgcacg atccggatcg
     4201 aaaaatacct gcacgatacc ttcgcggtcg cgcatatcga tgaagatcag gctaccaaga
     4261 tcacgacgac ggttgaccca accacacaga gtcacctgct gccccacgtg ggacaaacgg
     4321 agctgtccac aatattctgt acgcatgaga tatcccttaa cttagctgcc ggcggatgcc
     4381 ccctgctgcg caggtgacca agtcgcagcg ttagctgtat gtcacaactg aatgaaaaaa
     4441 ggcggctatt atactggaaa ttctgccgca ccgtaagagc ctggcccgcg ctggaacgcc
     4501 tcgttaccac tttatatcgg gcctgaaatc agactctacg ccagtttgct ataaaggtgt
     4561 tgcccgaact cataaaaatt aacaaaattt gtcgttccgc catcggctaa tcgcattaag
     4621 gtgagaggca cgattttgtt ttgtcaggag tcatcatgct tgaacttaat gctaaaacca
     4681 ccgcgctggt ggtgattgat ttacaagaag gcatcttacc ttttgccgga ggtccacata
     4741 ctgccgatga ggtggttaat cgcgccggga agctggcggc gaaatttcgc gccagcggtc
     4801 agcccgtgtt tctggtgcgc gttggctggt ctgccgatta cgccgaagca ttaaaacagc
     4861 cggttgatgc cccctccccc gcaaaagtgt tgcccgaaaa ctggtggcaa catcctgctg
     4921 cattaggtgc aaccgacagc gatatcgaaa tcatcaaacg tcaatggggt gcgttttacg
     4981 gtacggatct ggagttgcaa ttacgccgcc ggggtatcga tacaatagtg ttatgtggga
     5041 tctcgaccaa tatcggtgtt gaatccaccg cccgcaatgc ctgggaactc ggttttaatc
     5101 tggtgattgc cgaagatgcc tgtagcgccg ccagcgccga gcagcacaat aacagcatta
     5161 atcatatcta cccgcgcatc gcccgtgtgc gcagtgtgga agagatcctc cacgcgttat
     5221 gatttacatc ggtctgccgc aatggtcgca tcctaaatgg gtgcggttgg ggatcaccaa
     5281 ccttgaagag tatgcccgcc actttaactg cgtggagggt aacaccacgc tttatgccct
     5341 gccgaaaccc gaggttgtcc tgcgctggcg tgagcagacc acagatgact tccgcttctg
     5401 ttttaagttt ccggcgacca tttcgcatca ggcagcatta cggcattgcg atgatttagt
     5461 gactgaattt ttgacccgca tgtcaccgtt ggctccgcgc attggacaat actggctgca
     5521 actgcctgcc acattcggcc cacgagagct gcctgcgctt tggcattttc tcgattctct
     5581 tcccggtgaa tttaattatg gggtggaagt ccgccatcca cagtttttcg ccaaagggga
     5641 agaggaacaa acgcttaatc gcggtttaca tcagcgcggc gttaatcggg tgattttaga
     5701 cagccgcccg gttcatgcag cacgtccaca cagtgaagct attcgcgacg ctcaacgaaa
     5761 aaaacctaaa gttccggtac atgctgtact gacggcgaaa aatccactga tccgttttat
     5821 cggtagtgat gatatgacgc aaaaccggga gttatttcag gtctggttac aaaaattagc
     5881 acagtggcat cagaccacta cgccttatct ttttttacat acgccagata ttgcccaggc
     5941 cccggaactg gtacataccc tgtgggaaga cttacgtaaa acgcttccag agatcggagc
     6001 agttccggct attccacagc aatcttctct tttctgaatt tgccacctat catagacagg
     6061 tgccatcggc cattttaaag ggagtttgta tggtaagcgc gctgtatgcc gttttaagtg
     6121 cgttgttatt aatgaagttc tcttttgatg tcgttcgcct gcgaatgcag taccgcgttg
     6181 cctatggcga cggcggtttt agcgaactgc aaagcgctat tcgcattcat ggtaacgcgg
     6241 tggaatatat tcctatcgcg attgtgttga tgctgtttat ggaaatgaat ggcgcagaaa
     6301 cctggatggt gcatatttgc ggcatcgttt tgcttgctgg tcgtctgatg cattattacg
     6361 gttttcatca ccgtctgttc cgctggcgac gttctggcat gagcgcgacc tggtgtgcgc
     6421 tgttgctgat ggtgctggcg aatctttggt atatgccctg ggagttggtt ttctccctgc
     6481 gttagcgcac aatacgccac tttctttttc ccggattttt acgttatgtc tcaccgcgac
     6541 acgctatttt ctgcccctat cgccagactg ggcgactgga cctttgatga acgggtagct
     6601 gaagtcttcc cggatatgat ccagcgttcc gttcccggct attccaatat tatttccatg
     6661 attggtatgt tagccgagcg cttcgttcaa cctggtacgc aggtttacga tctgggttgt
     6721 tctctgggcg cggcgacgct ctcggtgcgt cgcaacattc atcatgataa ttgcaaaatt
     6781 attgccatcg ataactcccc ggcgatgatt gaacgctgcc gtcgtcatat tgacgcctat
     6841 aaagctccta cgccagtaga cgttattgaa ggtgatattc gcgatatcgc cattgaaaac
     6901 gcatcgatgg tggtgctgaa ttttaccctg caattcctgg aaccttccga gcgccaggcg
     6961 ttactggata aaatttatca agggctgaac ccgggcggcg cgctggtgct ttcggaaaaa
     7021 ttcagtttcg aagatgccaa agttggtgaa ctgttgttca acatgcacca cgactttaaa
     7081 cgtgccaacg gttacagcga actggagatc agccagaagc gcagcatgct ggaaaacgtg
     7141 atgctgaccg attccgtgga aacccataaa gcacgcctgc ataaagctgg ttttgaacat
     7201 agtgagctgt ggttccagtg ctttaacttt ggttcactgg tggcattaaa agcagaggac
     7261 gctgcatgat cgactttggt aacttttatt ctctgattgc caaaaatcat ctttcacact
     7321 ggctggaaac gctgcccgcg cagattgcta actggcagcg cgagcagcag cacgggctgt
     7381 ttaagcagtg gtccaacgcg gtggaatttc tgcctgaaat taaaccgtat cgtctggatt
     7441 tattgcatag cgtaaccgcc gaaagcgaag agccactgag cgccgggcaa attaaacgca
     7501 ttgaaacgct gatgcgcaac ctgatgccgt ggcgcaaagg gccgttctca ctgtatggcg
     7561 tcaacatcga taccgaatgg cgttccgact ggaaatggga tcgcgttctg ccccatcttt
     7621 ctgatttaac cgggcgcacc attcttgatg tcggctgtgg cagcggttat cacatgtggc
     7681 gcatgattgg cgcaggcgcg cacctcgcgg tgggaatcga ccctacgcaa ctgtttctgt
     7741 gccagtttga agcggtgcgt aaactactgg gtaacgatca gcgcgcacat ttgttaccgt
     7801 taggtattga acagcttccg gcattgaaag cctttgatac cgtcttttcg atgggcgtgc
     7861 tttatcatcg tcgttcaccg ctggagcatc tctggcagtt aaaagaccaa ctggtgaatg
     7921 aaggtgaact ggtgctggaa acgctggtta ttgatggtga tgagaacacg gtgctggtgc
     7981 cgggcgatcg ttacgcgcaa atgcgtaatg tctatttcat cccttccgcg ctggcgctga
     8041 aaaactggct gaagaagtgt ggttttgtag atatccgcat tgcggatgtg agcgttacca
     8101 ccacagaaga gcagcgacgc accgaatgga tggtcaccga gtctctggcc gattttctcg
     8161 acccgcatga tccgggtaaa acggtggaag gttatcctgc gcctaaacgc gcggtgctga
     8221 ttgcgcgcaa gccgtaaagg tctggtatta ctgccggatg cggcgtgaac gccttatccg
     8281 gcctacaaaa ccttgctaat tcaatatatt gcagggacta tgtaggcctt ataagcatag
     8341 cgcatcaggc agttttacgt ttgcataacc tcagcgcccg tttccgggcg cttttcacgt
     8401 cttatgcctg aacagctggc tgatcaaacg ccgttaactt cggtgcattg cccgtatatt
     8461 tttcgatata caccagcgca gagttaccgg cacaggcatt tgccagctga gagctgggga
     8521 tatccgccgt taacacgttc gcactgccgt ttttacacaa gccattttcc agatctggcc
     8581 atgcaccttc atgcacgcat accacgcctt ttttgatccc gtcagtcacc accgcgcctg
     8641 tcagaatctg accacgtttg ttccagacac gcaccagatc gccattcgca ataccaaagc
     8701 gagcagcatc ttcggtgtga atagtgatcg gttcacgatc ggcaaccgca tattttttac
     8761 gcagttccgc atagttaagc tgactgtgta aacggtgtgc cggatgtgcg gtcagaagct
     8821 gcaactgctt ctcgtcggcg gaacctttcc actcatcagg cgcaagccag gttgggtgtg
     8881 ccgggcaatc cttatagcca aatttttcca gcgttttgga gtaaatctca attttgccgc
     8941 ttggcgtacc cagcgcattt ttcaccggat cggcgcggaa atcaccataa cgaacgtatt
     9001 gttcgttctt ctcactgcgg cgcatttcga tcagtttatt ttgctgccag aaagcattaa
     9061 acatcggcat agtcacgcgt tgcgcacggg cacctttctg agcggcatca tagaaaaatt
     9121 tcagccacgc catttcatct ttaccttcgg tatagatctc ttttccgcca ggtttgagta
     9181 attcagcaag atcggcaaac acgtcaaaat cgttgcgcgc ttcaaattgc ggagcgacag
     9241 cctgcttcat cggcacaata tgctggttgc tgtaatcacc ggtcatcgtc aggtcattgc
     9301 gctcaaacga tgtggtgatc ggtaatacga tatctgcatg tttagcagct gcggtccagt
     9361 agcattcaga aacgacgatc atctccggtt tctgccatgc tttaatcaga cgattagtat
     9421 cctggtgatg ggtaaagtta ccgccacccg cccaccagat cattttgata ttcggatacg
     9481 tttgttcttt accgttatgc tgatatttgc cgccaggatt ttccagcgca tcgacgatac
     9541 gggcaacagg aatcgccgtc attccgccat catcagcggc ctcgctggcc tgtccggcaa
     9601 tcgccgcaga catttccggc agcacgccgc caacacgcgt cgggttgcca ccgttggagt
     9661 aatgataaga gaatccaaaa ccgccgcccg gtgtaccaat ttgccctaac atcgccgcca
     9721 gcgtcaccag catccagtgt ttctgttcac cgtattgctg gcgttggatc ccccagcctg
     9781 ccatcagcat cgtgcgattc gccgccatca gttctgcaag tttgacaatc tgcgcttccg
     9841 gtacgccagt aatttccgcc gcccaggccg cgctcttcgg cgtattatcg ctcttacctg
     9901 tcagatactc ttcaaactgc ggatacccgg tagtgtattt ttcaaggaac actttatcgt
     9961 gtttgccttg tgtcatcagg gtatgcgcaa tccctaacat cagtgccacg tcggtgccca
    10021 tattcggtgc gatccaggtg gcattatcgc caaagaattc gatggtttcg gagcagatag
    10081 gatcaatggc aatcactggt ttgccagatt ttttcagctg atggaagtat tccagccctt
    10141 gctcatcggt actgctccag gcaattttga gcgtgttcag cgggttcatc ccccacagca
    10201 ccacaacctg gctgttttcc agaatcagcg gccaggaggt ctgctgttca tacacctcta
    10261 cagaaccgac cacatgcggc atgatcacct gtgccgctcc ggttgaataa tcgccgctat
    10321 gcccggaata accgcccgcc aggttcatat aacgttgcag taaggtttgc gctttatgca
    10381 acacgccaga agaacgccag ccgtaagatc cggcaaaaat ggccgatggt ccgttagctt
    10441 tacgaatacg atcgtgttgc tcatgaatca gttttaatgc ttgttcccag ctcacctgta
    10501 cataggtatc ttcgccacga cctttcgccg gttgcagtgg attatcgaga tagctttttc
    10561 tcaccatcgg atgctgaata cgcgccgtgg tgtgtacctg atccgccgcc gtagactgta
    10621 aggagttcgg tatggttttc gccagcgcgc ctgtcgaaga aacaatcttg ccgtccttca
    10681 cttctacgtt catcgctccc caacggcccg cggtgagaat tttgccgccc ttctcttctg
    10741 cccatgcagg taacggtgca gccgatgtca ccaccagcgc ccctgccgca ataccgctat
    10801 gtttaataaa ttcacgtctt gttaatgtca taacttcctc cctgatcaac gaggatcact
    10861 gtttctcggt aatatctttg gcgttgtact ggaagtaccg cgtcaaaatg tccagttcgt
    10921 tttcgctcat gctggttcgc gcacccattc ctttggcaat ggacggccat gcattgacgg
    10981 tgtaatggtc ggaggcaata ggggcatggc aaccagcgca ataggtatcg tcaagttttt
    11041 cagcgtattg ccatagaggt ttacggtccg ctaatgcggg atcggtaagc acaccctgta
    11101 aagacgcctg acgccattga ttgccgtatt cgtcagcctg ccattccccg tttaccgtca
    11161 gcgccttgat accttcttca cttaatgtgg ctagcgccag ccgttgacct gccgccaggt
    11221 agagcgtgtt ttcactgccc tgcatttgat aaccctgcaa cagaacgatc ggctgtttgc
    11281 cactggcatc aacgacggtg agatcggtac caggattcac ggtagccagt tcgcctatgc
    11341 gagaagtttt gaaaggataa atatgtgcgc cattagtaac tgaagtagcg gcctgacttt
    11401 ccagctcatg cgccgcgttg tcatccattt ttatttctgg cggaaaatgg gcaatgcctt
    11461 tatggcaatc gatacaggtt tcgccgtcct tttgcgcttt attatgcatt ttctgcgcag
    11521 attcactttg cgaggcaata tccatggcat caaaagaatg gcaactacgg caggttgcag
    11581 agtcagttgc ttttaattct ttccatactg tttcggccat ttcctggcga tgagcttcga
    11641 acttatcatc actgtctatt ttgccgctaa caaattcatg ataaatatct ttagatgctt
    11701 ttaatttagc aaataaataa tccatccctg actttggaat atggcaatcg gcacattccg
    11761 cacgtatccc tttctggttt gaaaagtgga cagttccctg atattcctca aaaggtttac
    11821 tcatcgagtg gcaggaaagg caaaatgcgg tatccgacgt tttatgtaag gctttttgcg
    11881 ccagtaataa cccgccgcca ccaaccacaa ccgctatcag caaaaacaat aacccaatgc
    11941 gttttttccc tcgcatagat tcctctaatt aaattccagg acaccgatac cattgtataa
    12001 ataaatacac aacgaattga ctgcttgctt aattcggcac aacactgcct gaaacaatcg
    12061 ataaagaata tgatttatta caatgtaatc attaattgct aaggaataac ccagttgtta
    12121 tttagaatat atatttgtca gttttggcaa tttaaatcac ataatgaatg tgaatatata
    12181 aaatattttt agcacaacgc tatgcgcact gtcacccgct caacagatca cggcaatcgt
    12241 tcgtttttta tactgctcag ggataaaaaa aggcccctgt tgaaattgca ggggcctggt
    12301 acgagcaagc atcatattgg gcgacatgat gcaacggtaa aaatcatttg gcctgatggc
    12361 gttcaatgat tcctttcatt tcagcaaccg ccgccccgtc tacgacataa cgcgaatact
    12421 cgtccgcgtg tgcatctgat gacatggaca atccttgatt acgataacgc atcggtgagg
    12481 cttgccacgc tcccgcggag ctatggactt ccagcactcc ggcatcgagg aagtggtgca
    12541 ggttttccgc acggactcct gctccggcca taatgattgg agcatcacga tgggcaataa
    12601 gttccataat ttttgataaa ccttgcagtg cgtctgattt ttgccctgat gtcagtaccc
    12661 gggcaatgcc caattccgca agattattga gtgtatttaa agggttagcg cacatatcga
    12721 aggcgcgatg aaaagtcact gccagcggac cggcagcagc cattattttt tccattcgtg
    12781 gcatatcgac attcccgtca acatcgagaa cgcccgtcac cagtccagga aaacctaatt
    12841 cgcggactgt gcgcacatcc tcaagaatgg cggcaaattc accgtcgctg tagcaaaaat
    12901 caccaccgcg tgggcgaata ataggatgca cagggatcgt cacccgctgg cgtacggatt
    12961 tcagtacccc caacgacggc gttaagcccc cctcttttgg ggctgcgcat aattcaactc
    13021 tgtctgcgcc gttttgctgc gccgttagcg cgcattccat gctgtaacag caaatttcca
    13081 gtaatgccat ttttgctcct taattacgcc gactgctcgc tggcaacgat ctcttcaatg
    13141 gaccacggat gaaatttaat ggtcgttttg ccatcggtga ccgccagcgt cggattcggt
    13201 aatcgctcat gctcaccttt tggcgaactg gttttaactg agatccccgg caggctgagt
    13261 ccttcatcag aaagcaatgc cagcgcacgg gcattcagcg tttctggatc gcccggcaga
    13321 acgatttcaa tatgttccca accttcgtgc gggtaacgtt tttccccggg ccacggtagc
    13381 tccacaatgg aaaactgcca gtgagcaacc tgtaccggtt catgcaattt aaacagacaa
    13441 atcggcctgc cattgatcat attttctgac aaaagctcgc cgcactgttc aaacccgcga
    13501 cgccagcgct cagcagtggc gttttgatgg caacgcaaag aaatgtgatc ggcagtcagc
    13561 ggagtgatat ccaaccccag acggcgggaa agttcatcta atgcgtgggt aaatcgcggt
    13621 aaatccgatg caatatcctg caactcgttg atagattgcc agttcgccat aatcactctt
    13681 cgtcgttcaa taaaagcgtt aatttaccct gttgccctgc gccaaccaac cgctgattgc
    13741 acgccgcttc tgatgcaata gtgaaaacgg caatacgcca cgcgcacgtt gctgacgaaa
    13801 acagccattt gcagtatact cccgccctaa tttctttaac tggtgcgggc aaattttgct
    13861 cgcttcatca atgtaaggta ttccggtgaa tattcaggct cttctctcag aaaaagtccg
    13921 tcaggccatg attgcggcag gcgcgcctgc ggattgcgaa ccgcaggttc gtcagtcagc
    13981 aaaagttcag ttcggcgact atcaggctaa cggcatgatg gcagttgcta aaaaactggg
    14041 tatggcaccg cgacaattag cagagcaggt gctgactcat ctggatctta acggtatcgc
    14101 cagcaaagtt gagatcgcgg gtcctggctt tatcaacatt ttccttgatc cggcattcct
    14161 ggctgaacat gttcagcagg cgctggcgtc cgatcgtctc ggtgttgcta cgccagaaaa
    14221 acagaccatt gtggttgact actctgcgcc aaacgtggcg aaagagatgc acgttggtca
    14281 cctgcgctct accattattg gtgacgcagc agttcgtact ctggagttcc tcggtcacaa
    14341 agtgattcgc gcaaaccacg tcggcgactg gggcactcag ttcggtatgc tgattgcatg
    14401 gctggaaaag cagcagcagg aaaacgctgg tgaaatggag ctggctgacc ttgaaggttt
    14461 ctaccgcgat gcgaaaaagc attacgacga agatgaagag tttgccgagc gtgcacgtaa
    14521 ctacgtggta aaactgcaaa gcggtgacga atatttccgc gagatgtggc gcaaactggt
    14581 cgacatcacc atgacgcaga accagatcac ctacgatcgt ctcaacgtga cgctgacccg
    14641 tgatgacgtg atgggtgaaa gcctctacaa cccaatgctg ccgggtatcg tggcggacct
    14701 gaaagccaaa ggtctggcag tagaaagcga aggcgcgacc gtcgtattcc ttgatgagtt
    14761 caaaaacaag gaaggcgaac cgatgggcgt gatcattcag aagaaagatg gtggctatct
    14821 ctacaccacc actgatatcg cctgtgcgaa atatcgttat gaaacactgc atgccgatcg
    14881 cgtgctgtat tacatcgact cccgtcagca tcaacacctg atgcaggcgt gggctatcgt
    14941 ccgcaaagca ggctatgtac cggaatccgt accgctggaa caccacatgt tcggcatgat
    15001 gctgggtaaa gacggcaaac cgttcaaaac ccgtgctggc ggtacggtga aactggccga
    15061 tctgctggat gaagcactgg aacgcgcacg ccgtctggtg gcagagaaaa acccggacat
    15121 gccagctgac gagctggaaa aactggctaa cgcggttggt attggtgcgg tgaaatatgc
    15181 ggatctctcc aaaaaccgca ctaccgacta catcttcgac tgggacaaca tgctggcgtt
    15241 tgagggtaat accgcgccat acatgcagta tgcttacacg cgtgtattgt ccgtgttccg
    15301 taaagcagaa attgacgaag agcaactggc tgcagctccg gttatcatcc gtgaagatcg
    15361 tgaagcgcaa ctggctgctc gcctgctgca gtttgaagaa accctcaccg tggttgcccg
    15421 tgaaggcacg ccgcatgtga tgtgtgctta cctgtacgat ctggccggtc tgttctctgg
    15481 tttctacgag cactgcccga tcctcagcgc agaaaacgaa gaagtgcgta acagccgtct
    15541 gaaactggca caactgacgg cgaagacgct gaaactgggt ctggatacgc tgggtattga
    15601 gacagtagag cgtatgtaat cgcgttatac ggcaaaagcc gatgtcatgg cagacatcgg
    15661 cttttttatc attaacgata attcacaatc acttcattac gttgtacttt cagcggcgga
    15721 attaaccgcc caccgccagg cacttcccag ataaatcgca acggttctgc tgccgctata
    15781 ccggaaaagg ccacggtggt tccactctgc ccttctaatt cgacacaacg agattgtgaa
    15841 cacaagcgaa cccgcagtcc tgcaggtgtc gggccgataa gctgataacg ccacgccacc
    15901 agcgtcatca atcctgcagc aggttgtcga gtagaaagag gcgctgacga catcgactca
    15961 ccacggtgat tcagcgtaac gcccacactg ctcgcctgcc acatcccctc cccggcggct
    16021 tgcaccagca gcggaaacaa taatattgct aataaagttc tcatcattta ccaccaattg
    16081 tcgccgtcat gcggatatgt cggttatcag atagttccag attcgacagc accactaact
    16141 gcggcaggct gcggcgcagg aagcgagaca ataatggtcg cagcgcgtgg ttcactaaca
    16201 ataccggcgg cgcacccaac atctcctgac gggatagcgc ttcctgagtt tgcgccagta
    16261 aacgatccgc cagccctggc tccagtcccc ccccgccctg caacgcctgt agcaacaaac
    16321 gttccagcgg tgtatcgagg ccaataacat ggacttcatc tttgccagga aaccactgct
    16381 gagtaatcgc ccgtcccaac gctacgcgca cgacggcggt taattcatgc ggatcgcttt
    16441 ggatgggcgc atgttccgcc agcgtttcga gaatggtgcg catatcgcga atcggcactt
    16501 tttcatcgag cagattttgc agcactttat gcagtgtggt aagcgtgacg acgccaggaa
    16561 cgagatcttc cgtcagcttt ggcatctcct gggcgacgcg atccagcagc tgttgtgcct
    16621 cctgacgacc aaacagctct gcggcatgct ggctaatgag gtggttaaga tgggttgcca
    16681 ccaccgtgct ggcctcaacc acggtgtacc cctgaatctg cgcctgttct ttaagcgcac
    16741 tttcaatcca gatagcattc aggccaaatg ccggatcgac ggtcgcctca ccaggtaacg
    16801 tcccggcagc ggtaccaggg ttaatcgcca accaacgccc cggataagca tcaccactgc
    16861 ctatctccac gcctttcatc aaaatgcgat agcgggcagg ttgcagatcc atattgtcgc
    16921 gaatgtgcac caccggcggc agaaacccca tctcctgggc aaatttcttg cgaatactgc
    16981 gtatacggcc cagcaactca ccatcctgct ggaaatcgac catcgggatc aggcgataac
    17041 ccacttccat tcccagagaa tcttccagtt gcacatcgtt ccacgtcgct tcgacaacgg
    17101 tattattctc tgccattttt accggttttg gttcggcagg cgctttttgt tcacgtccgc
    17161 ggatccacca ggccagtccc agcaatccgg cagtgaacag caaaaatacc aggttcggca
    17221 ttccaggcac caggccgagt aaaccgagca cggcggcgct taacaacata acgcttgggt
    17281 tactgaaaag ctgattcacc atctgctcgc caacatcctg atcggtgctg acacgcgtaa
    17341 cgatgacccc cgcggcggta gaaatcacca acgccggaat ttgtgccacc agaccgtcgc
    17401 caatagtcaa tagcgtataa ctttccgccg cgtgtcccat gctcatacca tgttgcagca
    17461 cgccgaccag caacccaccg acaacgttta tgaccatgat gaggatcccg gcgatggcat
    17521 cgccgcgaac aaacttactt gccccgtcca tcgagccgta aaaatcggct tcctgagtca
    17581 cttcggagcg gcgttttttc gcttcatctt caccaatcaa tccggcgtta aggtcggcgt
    17641 caatcgccat ctgcttcccc ggcataccat cgagaacaaa gcgcgcgcct acttccgcaa
    17701 tacgcccggc acctttggta atgaccataa agttgatgat cacgagaatg acgaacacca
    17761 cgataccgat agcgaaattg ccaccaacga ggaagtgacc gaacgcttcg accacctttc
    17821 cagccgccgc cgcgccggta tgcccttcca tcaaaatgat acgggttgaa gccacattaa
    17881 gtgccagacg caacaacgtg gtaaacaaca gaatcgtcgg aaacgcagca aactcaagcg
    17941 tgcgttgggt aaacatcgcc accagcaaca ccatgatcga caaggcaata ttgaaggtaa
    18001 acagcaggtc gagtatgaat gcgggcagtg gcagtaccat catcgacaag atcaacagga
    18061 tcaaaatcgg tccggcaagg atctgccagt gtgtcgattt caggtttgcg ggcaggcgca
    18121 gcatcgcggc cagattactc atgggtcggt ttctcgttaa taaaatccag ggcttccggc
    18181 accggaagat gagtaggttg tacagggcgc tgtccaccag ccagacgcca gcgtttcagt
    18241 tgccagaccc aggccagcac ttccgccacc gcggcgtaca gttgacccgg gatttgttga
    18301 ccgatctccg catgtcgata cagcgcacgc gccagcggcg gcgcttcaag cgtcgggacg
    18361 ttattttcag cgccaatttc acgaatgcgc agcgcgacca gccctgcacc tttagcgacc
    18421 actttcggtg cgctcatttt gttttcgtca tactgcaaag ccactgagta gtgagttggg
    18481 ttattgacga taacgtccgc tttcggcaca tcggccatca tccgccgccg cgccgcagct
    18541 cgctgcatct gacggattcg ccctttgaca tgagggtcgc cttcgctttg tttgaactca
    18601 tcacgaatat cctgccgtga catacgcagc tttttcaggt ggctgaagat ttggaaaaag
    18661 acgtcaaatc ccaccatcgg aatgacacca agcaccacca gcaatgcgca cagccctacc
    18721 agatccatcg cattacccat ggcggtaatg ggggactccg ccattaagcg catcatttgc
    18781 ggccagtggt gccagagata aaaccccgtc acactgccga ccaggatgga tttcaaaatc
    18841 gctttaagca actccgcacc ggtttgtgcc gaaaacatcc gtttaatgcc cggtagcggg
    18901 ttgagtttgg aaaacttcgg ctgcaaggat ttgccgctaa ataccagccc tcccagcatg
    18961 accggagaaa tgagcgccac cagcaccacg ccgctaatca gcggcagcag cgccagcatg
    19021 gcttctctga tcagcagaat aatttgcccg aggatcagat tcgggtcatt gataatactg
    19081 tgatcaaaat gcagcccagc ggagagcatg cccgacaatc gacgggtcag cgacacaccg
    19141 ccaaaccaga taacactaac gcctaccagc aaaatcagca gtgaagtcag ttcacgggaa
    19201 cgcgggattt gcccctcttc ccgcgctttt tctagtcggt gaggtgtggg ggcttctgtt
    19261 ttgtcgtcgc tctcgtcaga cacatcgcca atcctggaaa gagtattaag cgtaaatgat
    19321 gccagagcgc aaagcgttca atggtttgag taaggggcaa aacaggcggg atttagggct
    19381 tttactgcca cacatcaagc atagtatgcg tttgtcggat gcggcgtcat cgcgttatac
    19441 gaccgcctga tatgacgtag tcacgccaca tcaggcaata caaatcaaaa tccaagacta
    19501 tccaacaaat cgtccacctg atcctgactg gctaccacac cggctttgct ggtatcgacc
    19561 tgaggtccat taagcaaact ctggttttca cgttttggac gcgactcctg ttcggggatg
    19621 ttttccaaca gcaccatcag caactgacgt tcgatctcct gaatgacatc catcatccgc
    19681 ttaatgacct gtccggtgag atcctgaaaa tcctgcgcca tcatgatttc cagcagctgc
    19741 gcgttagtaa agctggtatg cgcggggaca tctgccagaa attgccgcgt atctgttacc
    19801 agttctcggg cgtcggcaag gtcaatcgga tcggcaaacc agtcatccca acgttgggtt
    19861 aacgcttttg ctgatttctc catttgatcc tgatgcggtt gtgacgcctc aacactgttc
    19921 agcgcccgct ccgcagcctg ggcggtcatc tgcacaacat agtacaaacg atcgcgcgca
    19981 tcggggatgg cttccgccgc ttcggcaatg gcctgatcca gccccagctc ccgcaaactg
    20041 tcgcgcagca tacgcgtcag gctgccgata cgcgcaatga tatcgccagc tgaatgctcg
    20101 tcagcaggtt tgattgatgg ttgcatcata gtcgcatcct cacatgccca gtttctcaaa
    20161 gattttgttg agtttttcct ccagcgtcgc ggcggtaaat ggcttcacca catagccact
    20221 ggcccccgct tgcgccgcag caatgatgtt ctctttcttc gcttctgcag tcaccattaa
    20281 cactggcatt gccgacatcg cgccatctgc acgaatcgtt ttcagcaact ccaggccatc
    20341 catattgggc atgttccagt cggagataac aaatccataa ccgcctgcct gcaacttatt
    20401 gagagcgtcg acgccatctt ccgcttcctc aacattattg aatcccagtt ctttcagcag
    20461 gttacgcact atgcgtcgca tggtggaaaa gtcatccaca accaaaaatt taagttcttt
    20521 atccgccatt tcacactcct gatttaaata cgtatcgcct gtccggcact aatttttgcc
    20581 aacatttgct ggcttacctg gctaagatcg accacttcgc agacaccacc catattgatg
    20641 gcctcgcgcg gcatgccgaa caccacgcaa cttgcttcgt tttgcgcaag ggtccatgcc
    20701 cccgcctgac gcatcgccaa cattcccgcc gcaccgtcgt tgcccatacc ggtcaggatc
    20761 accccaaccg cattacgccc cgcctgtttg gcgacagaat ggaacaacac atctaccgaa
    20821 ggccgatgac ggttaaccgc cgggccatcg tgaattttga tttggtaatt tgcgccacta
    20881 cgcgccagct ccatatgccg atcgcccggc gcaatatagg catgccccgg taagacacgt
    20941 tctccgtctt cggcttcttt aaccccgatc tggcaaagct tattaagtct gtcggcaaaa
    21001 gagcgggtga aaccgggcgg catatgctgg gtaattaaca gtgccgggct ggaaagcggc
    21061 aacggttgca gtacgtgacg aattgcctca gttccacccg ttgaagcacc aatcgcaatc
    21121 agtttttcag aactcaacaa cggccccgct ttcagcgttg tcggtgccga caatggttta
    21181 tgtgccgtaa ggctcgcctt tgctgctgta cgcacctttt cagcaatcat ttcgctatac
    21241 gccagcattc cttcgcgaat acccaactgc ggtttggtga caaaatctat cgcccccagc
    21301 tccagcgcgc gcagcgtgac ttctgaccct ttgccggtca gggaagaaac catcactacg
    21361 ggcattggac gcaaacgcat taatttttcg aggaaatcca gtccgtccat ccgcggcatt
    21421 tcaacatcca gcgtcaatac atcgggattg aatttcttaa tcaaatcacg cgcaaccagc
    21481 ggatcagggg cggtcgccac catttccatg tcgctatggc tgttgatgat ttctgtcatg
    21541 atttggcgca tcagtgccga atcatcgaca gataacaccc tgattttgct catcgttaat
    21601 ccttacttag cgcatacacc gtctgaccac gcagcgtgaa gcggcgctca aggtggctaa
    21661 agttttcaga gtgacccgca aacagcaatc cgtcgggttt aaggagcgga acaaagcggc
    21721 gcaaaatctc ctgctgggta gtttgatcga agtagatcat gacgttacga cagaagatcg
    21781 catcaaacgg ccccggcacg gtgtactgtt tcgccagtaa attcagcggg gcaaaatcaa
    21841 catagttcgc cagctcctga cgcacgcgta ccagcccttc atgcggtccc gtccctcgca
    21901 tgaagtaccg ctgcagttgc tgcggcgtca ggtttttcaa ctcttcatgg cgataaatac
    21961 cgcttctggc tttttccagc acttcggtgt cgatatcact ggcaaacact ttccagcgtc
    22021 cgggcacggt gcccaatgtg tcagccagcg tcatcgcaat gctgtacggc tcttcgccgg
    22081 tcgaagccgc cgcgctccat acgcgatact cgccagaacg acgccgggcg tgatccgcga
    22141 gcagaggaaa atgatgcgcc tcacggaaaa aagccgtcag attcgtggtc agcgaattga
    22201 taaacgcctg ccactcaccg ctgtgctgat tagattccag caagttcagg taatggccga
    22261 aatccgtcag tcccagcgaa cgcaaacgac gaaccagtcg gttgtaaacc atgtcgcgtt
    22321 tatggtcagc cagaacgatc ccggctcgtt gatagatcaa ttgacttatc cgccgaaaat
    22381 gcgcgtcgga aagcgccagg cgctcggtca tctgtaacaa taaagacgtt tgcccacagg
    22441 gcagagatga agtcatagcg ccttctcaat cacttcagga taccactggc gcaatatgta
    22501 actgcgccga ctcatgtcgt gctacttcat gttcttcaag ggtaaatacc gccacacgcg
    22561 acgaaagatg gtcagcctgg ttcgccagtt gttccgttgc cgccgccgct tcttctacca
    22621 gcgaggcgtt ctgctgagtc acctgatcca tctggctgac agcctgtgca acctgctcaa
    22681 tcccccgcca ttgttcttcc gacgccgaag caatttctcc cataatgtcg ttcacgcggg
    22741 tcaccgaact gacaatatcg gtcatggtcg cggcggcgtt attcaccagt ttcgaaccct
    22801 gctggacacg attgactgac tcttcgatca gccctttgat ctcttttgct gcctgggcgc
    22861 tacggctggc aagattgcgc acttcacctg ccactaccgc aaaaccacgc ccctgctctc
    22921 cggcgcgagc cgcttccact gccgcattca gggccagaat attggtctgg aaagcaatgc
    22981 cgtcgataac gctgataatg tcgccaattt tttgcgagct ggtggcgatc tcctgcatgg
    23041 tgtgagtcat ggtactgacc tgaacaccgc ccgcctgcgc cgttgtcgcg gcattttttg
    23101 ccagttccga cgcctgtcgt gcgttatcgg cgttttgccc taccgtggcg gttaattgct
    23161 ccatactggc ggccgtttgt gccagcgatg ccgcctgctg ttcggtacga cttgagagat
    23221 cgttattgcc tgcgacaatc tccgcgatac caatgtgcat ctcctggctt cccttacgca
    23281 catcacttac cgtcccacgc aaagcctgct gcatggtctt cagactggca aaaatggcgg
    23341 tgatctcatt gcgaccatac actgcaatcg gacgcgccag attacccgca gcaatgctgt
    23401 caaaatggct accgatgatg gccagtggtt gaacaatcat cttgcgcgtc caccacagcg
    23461 cactgctgat gtagagcgct gcaacaataa tcatgctgat aaacaccagc gccgaaatgt
    23521 gatagttacg ctggctttgc gcactggcag cttccagcac atggttgatc tccagctgcc
    23581 aggcctcaaa gttaacgtca aacgcattct gtgattcctg caccggcgca gtgaggaaat
    23641 ccgaaagttg attactttca agccaggtcg cctgatgttc gagatcgttg tgccagcggg
    23701 caaaactttt ctccgtctct ttttgcaacg ccctgacgtg ctcgttgccc gcagtcatcg
    23761 ccataaaact tttaaacagc gtggtggatt gcgtcagact ggcgcgcgcc gtcgtcatca
    23821 acgttttaat gtcatccgcc ggatagctaa gcgccgtcag agtgcccgct ttgttcagcg
    23881 cggtactggc ctgtaacatt accgcccgag tttgcgctaa tgccgcacgt tgctgattgc
    23941 tctgctcgac ctgattcagt cgttgcaaat cgtcgcgaaa tgcccaaaaa gacatgccgt
    24001 tactgccaat ctgcaagatc ccgcagagaa tcaaaattaa aaacagcgtg gtcgaaattc
    24061 gaatacgatt aaacatcaac gctccccatc aggcggcaat gaccgcgtta gtaaaaactc
    24121 gtcaaaatgt ttcccagttt ggatcttgtt cagtaattcg cagtcgtggc tgtgccggtg
    24181 gttgctcact ggcaggacgg gatggtgttt gcggtttatt ggtgagtggg ctggctgcca
    24241 gacggaacgc ggaaaccgct tgcgttaaac gactcgcctg ttcttccagc gcagctgcgg
    24301 cggcagctga ttcctgcacc agcgatgcgt tctgttgcgt gacgcgatcc atttccgaaa
    24361 ccgccaatgc gacttgatcg atgccacggc tctgttcatc cgatgccgat gcaatctcgc
    24421 ccataatgtc agtcacgcgg gtgacagcat tgacgatatt gttcattgtt tccccggcgc
    24481 tttcgaccag caccgaaccg gtatcaacgc gcgagacgga gtcttcaatg agggctttga
    24541 tctcttttgc cgcctgggcg ctgcgactgg caagattacg cacttcgccc gccaccacgg
    24601 caaaaccacg gccctgttca cccgctcgcg cggcttcaac agcggcattc agcgcgagta
    24661 tattagtctg gaaggcaata ccatcgataa cgctgataat gtcggcaatt ttcttcgaac
    24721 tatcggcgat ctcatgcatc gttttcacta cgccatccac cactttgccg ccgtgctggg
    24781 cggtgtcgga ggcactttgc gccagttgcg aggcctggcg ggcgttatcg gcgttttgct
    24841 tcactgtcgc ggtgagctgc tccatgctgg cggcagtttc ttccagcgcg gatgcctgct
    24901 gttccgtacg ggaggaaaga tcggtgttgc ccgccgcaat ttcacgggta ccggcataga
    24961 tggcatctga accttcacgc acatgagtga cggtgtcagt caaagagcgt tgcatatgtg
    25021 aaacgctctg cgccaggtcg cccatttcac tgcgcccgtc aatggtcagg gtattcgcca
    25081 ggttaccacc ggcgatttcg cgaatgtgag caataatttt tgccagcggt gtaagcaaca
    25141 tacggcgaat gccgtaccac gccaccagca gaatcaatac caccaccagc gcgataaccg
    25201 ccagttgcca ctgggcaaat cggtaatcat ctgcgttgtc agtgacgata tcgcgataca
    25261 gtttttcact gctgagggcg tactgagcaa acgcttcgcc cattgcattt tgcattccct
    25321 gggttggctg agcgaaataa gctccagtat tgccataatc aagataatca atcagttcag
    25381 ttaacgctgt gtgatagttt ttatattttt catcaatatt acgactggta gcgaccattt
    25441 caggtaacgg tgccatgctt ttgaattttt tataatgagt cgctgcctgg gccaatgttt
    25501 tcctggcgct atcgagcaat tcaactttgg cattactttg ctgattggat gaatccatca
    25561 tcatccgcac cgctgaacga ctcaggttaa tgcgcgtttg cagcattaaa tcccatgttg
    25621 acgtcagctc gccctgctgt tcccgtaatt gattggaaac cacaaagctc ttctggctat
    25681 ggtgaaggga agaaaaaaac agactgccgg aaataagctg taacagtgcg aataccccca
    25741 gcaccattac caacagcgtg actacgcgga tacggttaat cataaggcac cttcctgaaa
    25801 acaagttgat ctcgttatcg gcaaggaagg gggaaacttt attgctgatg ccacccgccg
    25861 cgaaactgaa ataaaaaacc cgatgcgcag atcatcgggt tcattgcaat tgaggaaatc
    25921 gggagaatta cgccacttct gacgccgcgc tatctaatag cgccatctct tcgctgttca
    25981 gcagtttttc gatgttcacc agaatcaaca tacggtcgcc cagtgcaccc agtccagtga
    26041 gatattctgt tgaaagcgtc acggcaaatt ccggtgccgg acgaatttgc tccgccgtca
    26101 atgaaagcac gtctgagacg ccgtcaacca cgatgccgac cacccgctgt ccgagattca
    26161 ggacgataac taccgtgttg tcgttatagt ccacatccac ctggctgaac ttaattcgta
    26221 agtccacaat cggcacaata acaccgcgca gattcgtgac gcctttgata aacgctggcg
    26281 tgttcgcaat ccgtgttacc tgatcgtagc cacggatctc ctgcactttc agaatatcaa
    26341 taccgtactc ttcatcacca agggtaaata ccagaaattc ctggcctgac ggctcgctgg
    26401 ccagctttgt tacattcgtc ataccggtca tattgttacc tttttactca ttcaggcggc
    26461 ggtgttcgcc atacgttgtt cgcggtttat cgcctgcaag gcggagacat caacaatcag
    26521 tgccacgctg ccgtcgccaa gaatggtcgc agcagaaatg ccggggactt tgcgatagtt
    26581 actttcaagg tttttaacca caacctggtg ttgaccaatt aattgatcca ccagcaaggc
    26641 gtagcggcga ccgccacttt gtaagatcac cacaattccc tgggtagctt cggttttcgc
    26701 gcccgcgacg ttgaacactt tccacagttc gacgatgggc agatattcac cccgcacttc
    26761 cagcacccgc tcgccgccag ccagtggatg cagatcggct tcacggggtt gcagtgattc
    26821 catgacagca ttcagcggca gaatgaaaac ttcatccgca acgcgtacgg acatgccgtc
    26881 gaggatggcc agcgtcagcg gcagtaaaat gcggatcgtc gtgccagtac cctgcttcga
    26941 ctggatttcg acatgaccgc ccatctcctg gatattacgt ttaacgacgt ccatgccgac
    27001 gccgcgcccg gagacgtcgg tgacctgctc cgccgtggag aagccagggg caaatatcag
    27061 catcgcgact tcgtcgtcgc tcatgttttc gctgacggtc agaccttgcg aggccgcttt
    27121 tgccagaatt cgctcacggt ttagccccgc accatcgtcg gtcacttcaa tgcaaatgtt
    27181 gccgccctga tgttcggcag acagaattaa atttccgacg ctgtttttac ctgcggcgag
    27241 ccgtttttct ggcagttcaa taccgtgatc gaggctattg cgtaccaggt gggtcagcgg
    27301 gtcgataatg cgttctatca ggctcttgtc gagttcagtg gaactgccca ccagcgtcag
    27361 ttctacctgc ttgccgagtt ttcccgcaag atcacgcacc agccggggat agcgactaaa
    27421 gacatactcc atcggcatca tgcgaatcga catcactgat tcctgcaaat cacgggcgtt
    27481 acgttgtaac tgccccatgc tggtaatcaa atcaccatga ttaaccgggt ccagttcact
    27541 agaacgctgg gcaagcattg actgggtgat gaccagctcg ccgacgaggt taattaattg
    27601 atcaaccttt tctaccgcta cacggatgct ggtggattca ctgctgcgcg tcgttttttc
    27661 ccgctccacg cggccggttg gcgcttgttc ggctgccagt ttaagcactg gtggggggga
    27721 tattttgggc gagacttcta ctgtttcaaa ggtaatctga tcggcttcaa tcacaaaaca
    27781 gagtaccgct gtgatgtcat cttcggcgat gtcgcccggt aatattgccg agagagaatc
    27841 cgcccctttc accacatcag ttaacgttgt cagatgcccc agctcttctt ccagcaggtc
    27901 gacttccccg gccttcaggc gcgaaaggat aattcgtcgc ggcgactgat cgcggctctg
    27961 ctcatcttgc ggttcacttt tggcaaccac acttaatcgg gtcactgcag atggcgtttc
    28021 gcctttcgct tctaatgcca gttgacgcaa ggcctggcag atataatcga agctggcggc
    28081 atccggctct tgcgactgtt tataagcgtc gagctgttct tgcatgatgt ccttcgtttc
    28141 caaaaacaga ttgataatgt cggtgttgag ttgcatctca cctcgtctgg cttcgtcgag
    28201 caggttttcc atcagatgcg tggtttcctg caaaacgctg aagccaaagg ttcctgcccc
    28261 tcctttgatc gagtgggcag cccgaaagat ggcattcaat tgttcggcat ctggcgcttc
    28321 cggctgcaaa accagcagat gctgctccat gtcagccaac agttcgtccg cttcatcaaa
    28381 aaatgtctga taaaaatcgc ttatatccat gctcacgctg tcacctcggt tcggctgatg
    28441 gcattgtggg aacactgacc tgtggtgcaa cctcaggttc ttccagggcg cttactggct
    28501 cattctggct ttcggcgttt tcatgcaaaa tggcctgttc ggcttgtttg ttcagtacca
    28561 gcaggctgat gcgacggttg acggcatcat caggtccgcg atcgcttaag cgcatcgttg
    28621 ccgccatgcc gacgacacgt aacactttgc cgccattcaa cccgccaacc atcagttcgc
    28681 ggcgggatgc attggcacga tcggcagaaa gctcccagtt gctgtaacct ttctcaccgc
    28741 tggcgtaggg aaaatcatcg gtatgacccg agaggctaat tcggttgggg ataccgttca
    28801 gtacgggcgc gatggcgcgc agaatgtcgc gcatataggg ttcgacgtcg gcactgccag
    28861 ttctaaacat cgggcgattc tggctatcga tgatctgaat acgtagacct tcctggacca
    28921 gatcgatttt gagatgtgga cgtaacgccc gcagtttcgg atcggattca atcaactggt
    28981 cgagatcgcc acacaatttc cgcaatcgac tttgctccat gcgttttttc agctcttcga
    29041 tgttcggctg cttattcact tccccctgac tttgggtgta atcatcaccc ccaccgggaa
    29101 ttgggctttc actattagaa atgcgatcgc cgcccgtaac cgctgtcgcc agcggagtcc
    29161 ggaagtactc cgcaatctga atcagctctt ttggactgga gatagagatc agccacatca
    29221 ccagaaaaaa ggccatcatt gcggtcataa agtcggcata agcaatcttc cacgatccat
    29281 gtgctgctcc gtggcttttg gctttgcgtc gtttgacgac aataatcgga tgcgcttgat
    29341 tcttcatgct tcctcggttg tcgtctgttg ttgcggattt ttcaccgcac gcacatgctc
    29401 ttccagttca ataaacgacg gacgttcgct ggaatagagc gttttgcgac caaactcaac
    29461 ggcgataggc ggtgcgtaac cgttcagatt agatagcagt gtgactttga cgcactgcat
    29521 cattttgctg gtttcggcgc ttttctgacg taaaacactc gctaatgggg aaataaatcc
    29581 gtaagccaat aaaataccga ggaaagtccc caccatcgca tgtgcgataa gcgcccccag
    29641 ctcggcggca ggacgatcgg ctgaacctaa cgcgtgaacg acccccatta cagccgcaac
    29701 aataccaaac gccggaagtg agtcaccgac cagcgccaga ctgttcgccg ggacttctgc
    29761 ctcgctttcg tgcgtctcaa tctcttcatc catcagagct tcgatttcga aggtgttcat
    29821 gtgaccgctg ataatcaggc gcagataatc gacgataaat tcaagcatga cgctatccgc
    29881 aaggatgcgt gggtagctgg cgaagatctc gctctcacgg ggattttcaa tatcacgttc
    29941 cagcgaaaac atccccatct gccgcgattt cgccatcaac cgataaagca gagccagcag
    30001 atccatatac attgctttgg tgtatttgga gcgacgaaac agcaacggca gcgccttcag
    30061 cgtgccttta atcgctttgc cattattgcc gacgataaac gacccaatcc ctgcaccggc
    30121 aataatcacc agttcagcgg gttgatagag tgctccaagg cttccaccgg tcatcaaata
    30181 accgccgaaa actgtaccga gaacaaccag gtaacctaat aagataagca cgacatcatc
    30241 cttccactgt tgaccatgac aggatgttca gtcgtcaggc gttaacacgc gataggggca
    30301 aaaaaaagca gcggtacgtc gttaccgctg ctggaatgtt tcgccacacc gtatcagtta
    30361 aacagcctgt actctctgtt catccagcag ttgtgggata atatcggcag gattctggga
    30421 aagtttacgt ctttttactg cccgggatgg cggctgacat aagctgcagg caaagctgcc
    30481 aacaggctgg tgagcgtggg taataaaatt gccgccgcag cagttgcagc tggaaagttg
    30541 cagtaatcca ctttcaacaa accgcaccaa tgtccaggca cgggttaatg ccagcagcgg
    30601 tccctcttct gcttgcgggc actgttcaag gtataaacgg taggctttga tcaccgcatc
    30661 gacgccatta cacaaaccgg ttttcagtaa aaactgccat gcgttacaga acatcgaagc
    30721 atgaacgttt tgttcccagg tcataaacca gtcggttgag aatggcagca tgcctttcgg
    30781 cggtgggctt ccgcgcagtt ctttatagag ttttatcagg cgtccgcgac ttaactgtgt
    30841 ttcgctttcc agcatctgca aacgagcgcc cagggtgatc aattccattg ccagctgaat
    30901 atcccgcgct tcctgaacaa tgcttttttc actcatgatc aggccctttt cttgcgcagc
    30961 gcttcttcag gctgattaac atcattcagc aagcgtgttg agagcatgat gccggtatga
    31021 atttgctgga gatcgtcaac gcgggaatct tgcgtcaact gagtaatcgt ctggtgactg
    31081 tcaaaacgga agtgacaaac cagttgattg gtttctgcca gcttaaccat ttgcggaaga
    31141 gtcagtgccg ctaacgttgt cgccatttct tcatttatgc cgagacgaaa catagcggac
    31201 gctttgtcct gaacaatcag acgctgtgca agtagtaaat atgacaagtt gatgtcataa
    31261 atgtgtttca gcaactcgga ggtatgcatt attcccaccc agaataacca actttatttt
    31321 tatgcggtct caccgcaccc cgtgatgtcg ccgggaagcc ccggtaaaaa ataattagca
    31381 ttagaatagt tgcgataagc tgcaataggc agagcccctt tgtgggattt aataagtcct
    31441 tacaaataga aggggttctt tacacttatc taagattttt cctaaatcga cgcaacttta
    31501 ctcgtcacta cacgcacata caacggaggg gggctgcgat tttcaataat gtgtgatgca
    31561 gatcacacaa aacgctcaat tacttaacat aaatgtataa gtcatacttt tgttttgggt
    31621 gtatttcaat ctgttaaaaa gtttttcgcg acgctagcaa gcaaaaatga aacaggaata
    31681 atagaaatgg gatgttgcgc acagttaaaa taactcaccg taaataatca tctgctataa
    31741 ataattgctt taatgcaata ccagataa
//
LOCUS       DAPHCV010000054        30950 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.054, whole genome shotgun sequence.
ACCESSION   DAPHCV010000054 DAPHCV010000000
VERSION     DAPHCV010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.054"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            157..519
                     /locus_tag="RH033_002444"
     CDS             157..519
                     /locus_tag="RH033_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2991619.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKSMGKGREE"
     gene            complement(622..1080)
                     /locus_tag="RH033_002445"
     CDS             complement(622..1080)
                     /locus_tag="RH033_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDV2991620.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLLPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLSAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(1077..1994)
                     /locus_tag="RH033_002446"
     CDS             complement(1077..1994)
                     /locus_tag="RH033_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDV2991621.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            2140..2817
                     /gene="fetA"
                     /locus_tag="RH033_002447"
     CDS             2140..2817
                     /gene="fetA"
                     /locus_tag="RH033_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HDV2991622.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLCAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDTILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            2804..3583
                     /gene="fetB"
                     /locus_tag="RH033_002448"
     CDS             2804..3583
                     /gene="fetB"
                     /locus_tag="RH033_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HDV2991623.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(3646..4500)
                     /gene="cnoX"
                     /locus_tag="RH033_002449"
     CDS             complement(3646..4500)
                     /gene="cnoX"
                     /locus_tag="RH033_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HDV2991624.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(4561..5370)
                     /gene="ybbO"
                     /locus_tag="RH033_002450"
     CDS             complement(4561..5370)
                     /gene="ybbO"
                     /locus_tag="RH033_002450"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HDV2991625.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKADDVERMNSMGFTGVLIDLDSPECVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHCGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(5360..5983)
                     /gene="tesA"
                     /locus_tag="RH033_002451"
     CDS             complement(5360..5983)
                     /gene="tesA"
                     /locus_tag="RH033_002451"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HDV2991626.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQEVKATNAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            5954..6640
                     /locus_tag="RH033_002452"
     CDS             5954..6640
                     /locus_tag="RH033_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV2991627.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVNLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSVESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            6637..9051
                     /locus_tag="RH033_002453"
     CDS             6637..9051
                     /locus_tag="RH033_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2991628.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLMAI
                     LKTLGAGRAQLRKLIVGQWLMVLALSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(9764..10858)
                     /gene="mnmH"
                     /locus_tag="RH033_002454"
     CDS             complement(9764..10858)
                     /gene="mnmH"
                     /locus_tag="RH033_002454"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HDV2991629.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPHGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTQ"
     gene            complement(10927..11853)
                     /gene="allS"
                     /locus_tag="RH033_002455"
     CDS             complement(10927..11853)
                     /gene="allS"
                     /locus_tag="RH033_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HDV2991630.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLCQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVARLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            12083..12565
                     /gene="allA"
                     /locus_tag="RH033_002456"
     CDS             12083..12565
                     /gene="allA"
                     /locus_tag="RH033_002456"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HDV2991631.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            12643..13458
                     /gene="allR"
                     /locus_tag="RH033_002457"
     CDS             12643..13458
                     /gene="allR"
                     /locus_tag="RH033_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HDV2991632.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            13548..15329
                     /gene="gcl"
                     /locus_tag="RH033_002458"
     CDS             13548..15329
                     /gene="gcl"
                     /locus_tag="RH033_002458"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HDV2991633.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAVDAPTETCFMHYE"
     gene            15342..16118
                     /gene="hyi"
                     /locus_tag="RH033_002459"
     CDS             15342..16118
                     /gene="hyi"
                     /locus_tag="RH033_002459"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HDV2991634.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQILASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYEYLFTVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            16218..17096
                     /gene="glxR"
                     /locus_tag="RH033_002460"
     CDS             16218..17096
                     /gene="glxR"
                     /locus_tag="RH033_002460"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDV2991635.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            17266..18720
                     /locus_tag="RH033_002461"
     CDS             17266..18720
                     /locus_tag="RH033_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HDV2991636.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLVICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFMVMRGQINLDELYTAAGDFKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTAAEKTGEQKVTG"
     gene            18781..20142
                     /gene="allB"
                     /locus_tag="RH033_002462"
     CDS             18781..20142
                     /gene="allB"
                     /locus_tag="RH033_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HDV2991637.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDELGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMKAWGG
                     IAGLQSCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            20199..21500
                     /locus_tag="RH033_002463"
     CDS             20199..21500
                     /locus_tag="RH033_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HDV2991638.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPMSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            21522..22667
                     /gene="glxK"
                     /locus_tag="RH033_002464"
     CDS             21522..22667
                     /gene="glxK"
                     /locus_tag="RH033_002464"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HDV2991639.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDAHYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGITGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDAYGQV
                     LAANGGNLARVASIEMDECDPRLANCHIEVACDVNNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(22796..23581)
                     /gene="allE"
                     /locus_tag="RH033_002465"
     CDS             complement(22796..23581)
                     /gene="allE"
                     /locus_tag="RH033_002465"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HDV2991640.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYIPVEGHAPWLVSGNASELEHIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(23592..24827)
                     /gene="allC"
                     /locus_tag="RH033_002466"
     CDS             complement(23592..24827)
                     /gene="allC"
                     /locus_tag="RH033_002466"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HDV2991641.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCESEKQNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            complement(24849..25898)
                     /gene="allD"
                     /locus_tag="RH033_002467"
     CDS             complement(24849..25898)
                     /gene="allD"
                     /locus_tag="RH033_002467"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HDV2991642.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QKGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVNALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            26215..27882
                     /locus_tag="RH033_002468"
     CDS             26215..27882
                     /locus_tag="RH033_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDV2991643.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNHATPNDICVAIRSEAADAGIAQAVMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAATPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAETVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHQHGMMLDADGHQILDLGDDFYTVGRPHPMI
                     DPTLRNLLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSSATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            27892..29151
                     /locus_tag="RH033_002469"
     CDS             27892..29151
                     /locus_tag="RH033_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HDV2991644.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNKGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQAQAMLEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPHIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIFLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            29162..29977
                     /locus_tag="RH033_002470"
     CDS             29162..29977
                     /locus_tag="RH033_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDV2991645.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVVQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAVAWMQRAEETGLFGPLALAAGDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFIHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKS"
     gene            29974..30867
                     /locus_tag="RH033_002471"
     CDS             29974..30867
                     /locus_tag="RH033_002471"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDV2991646.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
BASE COUNT         7113 a         7653 c         8434 g         7750 t
ORIGIN      
        1 gctctttcaa tgtaaaaaag agtaaagact tgttttgata agcgaaataa attctgtttc
       61 tggactaaac gtgaagcggt tcctgatgtg tgctaaaatg cgtttctttg ataaatctca
      121 atttttaaga ggtttacgcc aggcaggaaa atttaaatga atgaatttga gaagattttt
      181 aatgaaatga atctggaccg ggcattgtta ccgatccttt ttcgttcgaa ccggtcgaca
      241 gtgtggaagt atttaagcgg cgatagtacg gctcctgcct ctgccatgtc gctgatcatg
      301 ctgttgcagt tgatccagaa acgaaacccc gatctgttag ccgaatggtt aacgctttct
      361 gatttcacca ttccgccaga agtttacctg gaccaacctg attactggaa agggtgggtg
      421 tatacccaac acaaggtcaa taaaaacgtc ctggaatatc taaaaaaaca ctatccggat
      481 gaagaccaaa aaagtatggg taaaggccgg gaagagtaat tttgcctcta ctcggctata
      541 tgccggatgc ggcgcaagcg ccttatctgg cctacgattc gggcatattt tgtaggcatg
      601 ataagacgcg ccagcgtcgc atcaggcgat gaccgcacgg ataatcagcg ttatcccttc
      661 tatcgcaatc acctcaacat gcgtacctgc actgagatcc tcgctggcgc tgacaggcca
      721 tgaactgtca ccgacgcgca tatgaccacg cccgttgacc agcggagatt ccagaacaaa
      781 acgtcggcca atcaactgct gcccgcgctg gtttaaatga ctgtcgctgt gcttttgttc
      841 gcgcacccgc cgcgacaacc atttccacca cagccaggcg gcgagcagcg tcaggacggc
      901 aaacattacc ccttgccact cccaacccag cggcagcaac cagaccacca gaccggtaat
      961 caccgctgcc acgccgctcc acaacagata accattgccg cccaacatct cggctgccag
     1021 cagcaaaccg ccgagactta gccagaaaat atgtggatga acgaccatta actccatcat
     1081 ggctgagtcc gtttgttggc gctgtctttc accagctcgg caatcccggc aatcgacccc
     1141 atcaggctgc tggcctctaa tggcatcatc actactttgc tgttactgga cgaaccgatc
     1201 tgctgtaacg cttcggtgta tttctgtgcg acgaagtagt tcaccgcctg aatatcaccg
     1261 gaggcaatgg cttcagatac cattttggtg gcgcgggctt ctgcttcggc agaacgttca
     1321 cgcgcttcgg cctgtaagaa tgccgattga cgttcacctt ccgctttcag gatttgcgac
     1381 tgtttttcac cttcagcttt gaggatttcc gcctgacgga tcccttccgc ttcaaggatg
     1441 taagcgcgtt tggtacgttc cgctttcatc tgcgcgttca ttgaagagat aagctctgcc
     1501 ggtgggcgca cgtcgcgaat ttcaatacgg gtgactttaa tcccccacgg gttggtggct
     1561 tcatcgacaa tacgcagcag gcgtgagttg atgctgtcgc gctgagagag catttcgtca
     1621 agttccattg aacccagcac ggtacggatg ttagtcatgg tcaggttgat gatcgccagc
     1681 tccagattgc tgacttcata agccgcgcgc ggcgcgtcaa tcacctgaat aaagcacacg
     1741 gcgtcgatgg taacgttggc gttatctttc gagataactt cctgggaagg gatatcgagc
     1801 acttgctcca tcatattgat cttgcgacca atgcgatcca taaacggcac caccagactg
     1861 agccccggct gtaacgtttt ggtatagcga ccaaaacgtt ctactgtcca ctgatagccc
     1921 tgcggcacga ttttgacgcc cgcgccgaca atgaccagcg cgacaaaaat gagaatcggg
     1981 ataaagataa gcatcggaaa aacctcctgt tgtaccgtcc ataatcagca aaattgctgc
     2041 ttgattaaac aaattatacc tgattactga aagagagttt ccccttattc ctgcgaagga
     2101 taaactgttt ttagtaaaaa tcagaaaaag ggaacagtga tgcaggaaaa tagtcctttg
     2161 cttcagctac aaaacgtagg atatctggcg ggtgatgcga agattcttaa taacatcaat
     2221 ttttcgctgt gtgctggcga atttaagtta attaccggtc cttctggttg tggcaaaagt
     2281 acgctgctaa aaatagttgc ttcattgatc agcccaacca gcggaacgtt actgtttgaa
     2341 ggtgaggatg tcagcacact aaagccagaa atctaccgcc agcaagtctc ttattgcgcc
     2401 cagacgccga cgctgtttgg cgacacggta tacgataatc tgatctttcc ttggcagatc
     2461 cgtaaccagc agcctgaccc agccattttt ctcgattttc tcgaacgctt cgccttaccg
     2521 gacaccattt tgacgaagaa tatcgccgag ctatctggtg gggaaaaaca acgcatctca
     2581 ttgattcgta acctgcaatt tatgccgaag gttttattgc tggatgaaat aaccagtgcg
     2641 ctggacgaaa gtaataaaca taacgttaat gagatgatcc accgttatgt gcgcgagcaa
     2701 aatattgccg tgctgtgggt gacacacgat aaagacgaaa ttaatcatgc ggataaagtg
     2761 attacactgc aaccgcatgc cggagaaatg caggaagcac gctatgaact cgcataatat
     2821 tactaacgaa tcattagcac tggcattgat gctggtggtg gtggcaatct taattagcca
     2881 taaagaaaaa ctggcgctgg agaaagatat tctctggagc gtcgggcgag cgataattca
     2941 gctgattatt gtcggctatg tgctgaagta tattttcagc gtggatgatg ccagcctgac
     3001 attattgatg gtgttattta tctgctttaa tgcggcgtgg aatgcgcaaa aacgcagtaa
     3061 atatattgct aaagctttta tctcatcgtt tattgccatt acggtcgggg cgggaattac
     3121 cctggcggtg ctgattctct cagggtcgat tgaatttatc ccgatgcagg tgatccctat
     3181 cgccgggatg atagccggca acgccatggt agcggtgggg ttgtgttaca acaatttagg
     3241 gcaacgggtc attagcgaac agcaacagat tcaggagaaa ctgagtcttg gcgcgacgcc
     3301 gaaacaggct tcagcgatat tgattcgcga cagtattcgc gcggctttaa ttccgacggt
     3361 cgattcagca aaaacggtag gcttagtgag tttaccagga atgatgtccg ggctgatatt
     3421 tgccgggatt gatccggtga aggcgattaa atatcagatt atggtgacct ttatgctgct
     3481 ctcaaccgcc agcctgtcga ccattattgc ctgctattta acctatcgta agttttacaa
     3541 ttcgcgccac cagttggtgg tgacgcaatt gaagaagaaa tgatgcgatg ccggatgcgc
     3601 agcatcgcat ccggcgttgt ggttcatgtg ccgatcaacg gaaactcaat acaacaacgc
     3661 atacagctgg cggcgatact tcgacgccag tgcatcaccc gtacccagcg cagcgaggat
     3721 ctcctggaac gttttacgcg tctgaccgtc tgcggcggtg agatctttac gcagatgccc
     3781 gaacagcaac tccagcgcct cttcattgcg cccaacctga tgcagttgca gcgccagttg
     3841 cgtcgccagt gcggcatctt ctggattctc cgccacctgc tgttgcaact gttgaatttc
     3901 cggcgtatca gccgcctgct tcagcagttc gatttgcgcc accagcccct ggtagcgggt
     3961 gtcctgatcc tgcaacggaa tggttttcag caccgcttct gcatcttcag aacggttcag
     4021 cgcaatcagc gtttctgcca gcagcaggcc gatctccccg ttctgattcg acaactgcca
     4081 ggcgtctttc agcaatggca aggcgtcggt gtaattgcct tcctgcatca gctgcatcgc
     4141 ctgctgcgct ttcagctctt cttcgcgcgg cagcacttta tccagcaggg cgcggatcgc
     4201 ctcttccggt tgcggcccct ggaagccatc taccggttgc ccgttctgga acagatacac
     4261 ggtcggaatc gcgcgcagac caaactgggc ggcaatcatc tgctccgcgt cgcagtccag
     4321 cttcgccaga ataaattgcc cgttgtactg cgccgcgagg ctttccagga tcggggttaa
     4381 ctgcaaacag tgctggctac gttcagacca aaaatagaac agcaccggag tggtcatcga
     4441 ctgttcaaga acctgttgca ggttagattc gttaatgttg acaatatttt ctacggacat
     4501 ggagtcgctc tctgttgtcg atattttctt tgacatgggg gcttaagcgc gcgcttcaac
     4561 tcacccctgc aatattttgt ccatcacgcg ccccggcagt aggcgcttaa gcaccattac
     4621 cgcccaggtc accagcgtta ccggatagcg catcttcggc ttctcgctaa taaaagcatg
     4681 gcgtactttg tccaccaccg cttccggtcc caacgtaaag cgggcggcga tgccgggatt
     4741 ttcgactggt ttatcacttt gcgtctggtt gacgttgtcg gtgaagcgag tacgaatggg
     4801 accgggttcg atcaggctga ctttaattcc acagtggcgt agctccatgc gcagcgcgtc
     4861 tgaccacgcc tccagcgcat atttactggc cgcgtaagcg ccacgacccg gtgtggagat
     4921 taatcccatc accgatgagg tcatcacaat acgcccttca ccgtgcggca acatcgcggg
     4981 taacaggcgc atggtgagct ggtgtgcgcc gaaaaagttg gcggaaaact gctgttccat
     5041 ctgcgcacgg ctgatggtgg aaagggggcc atacatgccg aatccggcat tgttaaagat
     5101 cccatacaga caattatcgg tcagggcgat cacttcgtcg gctgcgcgat caacacattc
     5161 tggcgaatcc agatcgatca acacgccggt aaatcccatg ctgttcatgc gctcaacatc
     5221 atccgctttc cggcaacctg ccagcacatg aaaaccctgg cgttttaatt cgagcgcgct
     5281 ttccaggcca attccactgg aacatccggt aattaagacc gatttttgca taactttacc
     5341 tgtcaggatc tccgttgctt tatgagtcat gatttactaa aggctgcaac tgcttcgcca
     5401 tccagtcggc aataaacggc tgggcgtcgc ggttgggatg aataccgtca tcctgcatcc
     5461 attgtggttt gaggtagacc tcttccataa aaaagggcag cagcggaaca tcaaactctt
     5521 tggcgagttt ggggtaaatg gcgctaaagg cttcattata acggcgacca tagtttgcag
     5581 gcagacgtat ttgcattaac aatggttcag cgttggtggc tttgacctcc tgcaaaatct
     5641 ggcgcagcgt ttgctcggtt tgttgtggct gaaaaccacg caaaccgtca ttgccgccca
     5701 gttcaaccag cacccaacgc ggctgatgct gtttcagcag agccggaagg cgcgccagcc
     5761 cttgttgcga ggtgtcgccg ctgatgctgg cattgactac cgacgtttta ctctgccact
     5821 tatcattcaa caaggcaggc caggccgcgc tggcagacat tcgatacccg gcgctcaggc
     5881 tatcacccag aatcaataac gtgtccgctg cagcggcacg gaaggttaac aggaccagga
     5941 acaggaaggg caaatgccag cggaaaacat tgttgaagtt catcatctta agaagtccgt
     6001 cggtcagggg gagcatgaac tctccatcct caccggagtt gagctggttg tcaaacgtgg
     6061 cgagaccatc gcgctggtgg gcgagtcggg atcgggtaag tcaaccttgc tggcgatcct
     6121 cgccgggctg gatgacggca gcagtggcga agtaaatctg gtgggacaac cgctacataa
     6181 tatggacgaa gaagcgcggg caaagttacg cgcgaagcac gtaggttttg tttttcagtc
     6241 atttatgtta attcctaccc ttaacgcgct ggaaaacgtc gagcttccgg cgctgctgcg
     6301 cggtgaaagt agcgtggaaa gtcgtaacgg ggcaaaagcg ttgctcgaac agttaggact
     6361 gggtaaacgt ctggatcatc ttccggcaca gctttccggc ggcgaacagc aacgagtggc
     6421 gctggcgcga gcttttaatg gtcggcctga tgtgctgttt gccgacgaac ccaccggcaa
     6481 tcttgaccgc cagacgggcg ataaaattgc cgacctgctg ttttccctca accgtgaaca
     6541 tggcaccacg ttgattatgg tgacccacga cctgcaactg gcggcacgct gcgaccgctg
     6601 cttacggctg gtgaacgggc agttgcagga ggaagcatga ttgcacgttg gttctggcgc
     6661 gaatggcgct cgccgtcgct attaattgtc tggctggcgc taagcctggc ggtggcctgc
     6721 gtgctggcgc tgggcaatat cagcgatcgc atggagaagg gcttaagcca gcaaagccgg
     6781 gagtttatgg cgggcgatcg ggcgttgcgc agttcgcgcg aagtgccgca agcgtggctg
     6841 gaggaagcgc aaaagcgcgg cctgaaagtc ggcaagcagc tgactttcgc caccatgaca
     6901 tttgcaggcg acacaccgca gctggcgaac gtcaaagcgg tggatgatat ctacccgatg
     6961 tatggcgatc tgcaaaccaa tcccccaggc ctgaaaccgc aggcgggcag cgtattgctg
     7021 gccccacgcc tgatggcact gcttaacctg aaaacgggcg acaccattga cgtgggcgat
     7081 gccaccttgc ggattgccgg agaagtgatt caggaaccgg attccggttt taaccccttc
     7141 cagatggctc cgcgtctgat gatgaatctg gcggatgtcg ataaaaccgg agccgtgcag
     7201 ccgggaagtc gggtcacctg gcgctataaa ttcggcggca acgagaacca gctcgacggc
     7261 tatgagaaat ggttgttacc ccagcttaaa cccgaacaac gctggtacgg tctggaacag
     7321 gacgaaggcg cgctggggcg ctcgatggaa cgctcgcaac agttcctgct gctttcggcg
     7381 cttctgacct tgctgctggc agtggcagcg gtggcggtgg cgatgaatca ttactgtcgc
     7441 agtcgctacg atctgatggc gatcctcaaa acgctgggag cagggcgagc gcaattgcgt
     7501 aagttaatcg tcggtcagtg gttgatggtg ctggcgcttt cagccgttac cggtggtgcg
     7561 ataggcctgt tgttcgaaaa cgtgttgatg gtgctgctca agcccgtttt gcctgctgca
     7621 ctaccgccag ccagcctctg gccgtggttg tgggcgctgg gcaccatgac ggtcatctcg
     7681 ctgctggtgg ggctacgacc gtaccgcttg ttgctggcaa cgcagccttt acgcgtatta
     7741 cgtaatgatg tggtcgcgaa cgtctggccg ctgaagtttt atctgccgat tgtcagtgtg
     7801 gtggttgtgc tgctgctcgc cggattaatg ggtggcagca tgctgctttg ggcggtgctg
     7861 gcgggcgcgg tagtactggc tttgctgtgc ggtgtgctgg gctggatgct gctgaatgta
     7921 cttcgccgca tgacgctgaa atcgctacct ctgcgcctgg cggttagccg cctgttacgt
     7981 cagccgtggt caacgttaag ccagctttcg gcattttcgc tctcctttat gctgctggcg
     8041 ctgttgctgg tattgcgtgg cgatctgctc gaccgctggc agcagcagct acctccagaa
     8101 agcccgaact actttttaat taacatcgcc acagaacagg tagcaccgct aaaagcgttc
     8161 ctcgcggaac atcagatagt cccggaatcg ttttatccgg tggtgcgggc gcggctgacg
     8221 gcgattaacg ataagccgac agaaggcaat gaagatgagg cgctcaatcg cgaactcaat
     8281 cttacctggc aaaatacgcg gcccgatcat aatccgattg tcgccggtaa ctggccgcca
     8341 aaagccgatg aagtgtcgat ggaagagggg ctggcgaaac gcttaaacgt tgccctcggt
     8401 gataccgtga cttttatggg cgatacccag gagttccgcg ctaaagtgac cagcctgcgc
     8461 aaagtggact gggaaagtct gcggcctaat ttctatttta ttttccctga aggggcatta
     8521 gacgggcaac cgcagagctg gcttaccagt ttccgctggg agaatggcaa cggcatgttg
     8581 acgcaactca accgccagtt ccctactatt agcctgttag acattggcgc gattttaaaa
     8641 caggtcggtc aggtgctgga gcaggtaagt cgggcgctgg aggtgatggt ggtgctggtc
     8701 accgcctgcg gtatgttgct gttgctggca caggtgcagg tgggaatgcg tcagcgtcat
     8761 caggagctgg tggtgtggcg cacactcggt gcggggaaaa aactgctgcg taccacgttg
     8821 tggtgtgagt tcgccatgct tgggtttgtt tctggcctgg tagccgcaat tggtgcggaa
     8881 acggcgctgg cagtgttgca ggcgaaagtg tttgattttc cgtgggagcc agactggcga
     8941 ttgtggattg tgctgccgtg cagcggtgca ctgctgctgt cgcttttcgg cggctggctg
     9001 ggtgcgcgat tggttaaggg taaggcgctg ttcaggcagt ttgcggggtg atgacaggcg
     9061 ttagattgcc tgatgcgcta cgcttatcag gcctacgagg atcgtgcaat atattgattt
     9121 tgcaagattg tgtaggccgg ataaggcgtt tacgccgcat ctggcatgaa cagtgtgcat
     9181 tttggcaata aatatgaaac caactgtcat gtcggttttt ttaaaggccg aaggtggtaa
     9241 accttcggcc ttagctacgt ttcctcagtt attcaataaa ctgacggatc agttccagca
     9301 gcgcgattag cgccttaaga acgatgagaa ctttatcgag aaaagttttc atccgttgct
     9361 cccttaaggg agaaagacaa tcacagctta cccttacgtt gcctgtaact atgattttct
     9421 tcatgtgcta gatgaggact gcctctctcc aaattagagc accagaatca gcaccacctg
     9481 tatcctgacc aggacttaaa agtacttttc cgcactgcgg tagtcccttg aggcagtatc
     9541 actactagcg tcgggattgt atcgccaaca tagctgtttg aatatgtagg catcctgact
     9601 tataattcac ttgctagatg aggactgttt catttctgcc cggaaccacc aagtcttggg
     9661 cggaaaaaat aaaaaaaccg actgtcatgt cggttttttt atatctgtat tctggtgcaa
     9721 ccattctgtt ctcacgacaa tcgccgtttc agaataccgc ccatcactgc gtcttaaccc
     9781 attccgccac ttccgcccac tcaccgcgaa agacaacttt ttccgctttt ttctcaagct
     9841 gatagcgata catcgggtcg taatattctt caagtaacgg caccagccag gccagatgac
     9901 cgtcggtgct gccggtggta agttgcgttg tcagcgccgc atccagcctt gcagccagtt
     9961 cgttatagcg ctgtagcccc agccgacgct taatcgccga aagtccgtga tgcaggtatt
    10021 cgcaatactc ctgccagccc tgttcgtcgc cgtacgcgtg ggtaaaatca tgatgcatac
    10081 gcaggaaata ttcttcgttc agccgctcaa gacggatctc aaacggatct tctactaccg
    10141 caatcgccgc ctgggtcatt cgctcgcgca ggcattccgg caggtgattc gaaccgatca
    10201 tccggctttc gtcttccagc acccacaggc gcaaattctg acgggcgtcg gtttttagca
    10261 tttcggcagc cagcaggttt tcaaaactcg cctggctaag ttgtggttgt aaggtgcgac
    10321 caaacgccga accgcgatga cgcgccaacc cttccagatc aacaccgttc ggctgttgct
    10381 gcactaacag cgttttaccg ctgccggtac aaccgccaat cagcactatc ggtttttgtg
    10441 ccagttcaat agtcgcctga atcgcggtct ggcgcagtgc cttataaccg ccttccacca
    10501 gcggataatc aatccccgct gcatgcaacc agctttgcac aatatgtgag cgctgaccgc
    10561 ctcgggcgca gcagagaata ccgtgcggat tttgcaggca cgctgcccgc caggcgtcca
    10621 tgcgctgctg acgaatttca cccgccacca gtttatgacc cagcgccagc gccgcgtctg
    10681 acccttgctg tttatagcag gtgccaacgg cggcgcgttc atcgttattc attaacggca
    10741 ggttgatagc ggcaggcatt gcgccttgct caaactcgat gggggcgcga acatcaataa
    10801 tgggcgtatc agcaatcagc agggcacgat agtcctgttc cgtgtgtctc tcttgcatag
    10861 taaaaagtga acctcaaatc agcttgcgcg ctattttacg cgccaacgcg caaggaaact
    10921 tgatttttaa ctgcgtgggt tgccgaaaat ttctaaaaat ccgctgattt ccggcctgcg
    10981 ctgggtaaac aaggtcacaa tatcttctac cgctttgccg ctgccaaatt tgcgccatgc
    11041 cagactcaat ggtgaaggag ggcgcatcgt tgggattacc cggctgacca gttgttgatt
    11101 atcgatcatt gactggcaaa gtgattttgg caaaaaacca atgccaacgc ccgccagatg
    11161 ggcggcgatt ttcgtttcca tatcgggaac aataatctct ttttgccccg gcaatcgcca
    11221 ggcgacgcgt ttggttagtg tgcgggcgct gtcttcaata ttaaccgccg gaaagcgccg
    11281 caactgtgct tctgttagcg gctcttcaac gttcgccagc ggatgatccg ccgccatgac
    11341 aaagcgccat tgcaccgatc ctaagggatc aagactaaag gtatttgcca gcgcctcggt
    11401 tcccgtgacg ccgatagcca gtgaaaaacc ttcgtataat agcgaatccc agacgcccat
    11461 atagatttgt cgggagatgt gaaattgggt aaacgggtaa cgttcattta gccacgccag
    11521 cagcctggca acggcctgag ggttatagag caggttgttg atgacgatat tcacctggcg
    11581 ttccacgcca tcattcacct gttgtaactc gctgggcata ctttccagcc agctcagcca
    11641 gtctctggcc tgacaaagta gatgctcgcc agccgccgtc aacgtcacgc tacgagtcgt
    11701 acggaaaaac agcgctactc cggtattctc ttccagaagt ttaatgcgat aactgatcgt
    11761 cgccgtggtt ttacataatc gttctgccgc ttttgaaaaa cttcctgttt cagcaaccgc
    11821 aatgaaagtc cgcaaggttt ctggatcgaa catcttcagg tatccccttt taaatccgca
    11881 agttgcgtga ttttcttatc ctctgattta tcagtatttt tacatgataa ccctgttcaa
    11941 tttgtggact aaatctagtt ttggaaaaat attccaactt ttgtattgat gttgttctct
    12001 taaggtttta gattgcctgt tattgaaacc aagctgaccg gtcggcggtg gttgaacgga
    12061 attatgttac aaggacaaaa agatgaaact tcaggtatta ccgttaagtc aggaagcctt
    12121 tagtgcttat ggcgacgtaa tcgaaacgca gcaacgggat tttttccata ttaacaatgg
    12181 cctggtggag cgttaccacg atttggcgct ggttgagatt cttgagcaag accgtacgct
    12241 tatcagcatt aaccgagcgc aaccggcgaa tctgccgctg accattcacg aactcgaacg
    12301 tcatccgctg ggtactcagg cctttatccc gatgaaaggt gaagtgtttg tggtagtcgt
    12361 ggcgttaggt gacgacaaac cagacctgtc aacgctgcgg gcgtttatta ccaacggtga
    12421 acagggggtg aattaccatc gtaacgtctg gcatcaccca cttttcgcct ggcagcgcgt
    12481 caccgatttt ctgaccatcg atcgcggcgg cagtgacaac tgtgatgttg aaagtattcc
    12541 tgaacaggaa ctctgttttg cgtgacgcct gcaaccgact tgcataagat aaacgaattg
    12601 ttcattgttt atgctcactt gtaggtcgga gttaacgtag gtatgacgga agttagacgg
    12661 cgcggcaggc caggacaggc ggagcctgtg gcacagaagg gcgcacaggc gttagagcgg
    12721 ggaattgcga ttctgcaata tctggaaaaa agtgggggaa gttcgtcggt tagcgatatt
    12781 tctctcaatc tggatttgcc gctctccacg acctttcgct tgctgaaggt tttacaggca
    12841 gcggattttg tctatcagga cagtcagtta ggctggtggc atataggatt aggtgtcttt
    12901 aacgtcggtg cagcgtacat ccataaccgc gatgtcctct ccgtcgccgg gccgtttatg
    12961 cgccgcctga tgttactttc cggcgaaacg gtcaatgtcg cgatccgtaa cggcaatgaa
    13021 gcggtattaa ttggtcagtt agagtgtaaa tcgatggtca ggatgtgtgc gccgctgggc
    13081 agtcgtctgc cgctgcatgc ttccggtgcg ggcaaggcgc tgctttatcc gctggcggaa
    13141 gaagagttga tgagcatcat tctgcaaacc ggtttgcagc agtttacgcc aacgacgctt
    13201 gtggatatgc ccaccttgct gaaggacctg gaacaagcgc gtgaactggg ctataccgta
    13261 gataaagaag agcatgttgt cggcctgaat tgcatagctt cagcaattta cgatgatgta
    13321 ggtagtgttg ttgccgctat ctccatctcc gggccttcat caagactgac agaagatcgt
    13381 tttgtcagtc agggtgagct ggtcagagac accgcccgcg atatcagcac ggcgttggga
    13441 ctgaaagcac atccatagtg tctgtcgtat cccgctctgc ggagcgggtt tttttgacaa
    13501 aatttgaaag ttggaaaaat tttccaatta atagaggtag gaataaaatg gcaaaaatga
    13561 gagccgttga cgcggcaatg tatgtgctgg agaaagaagg tatcaccacc gccttcggcg
    13621 ttccgggagc tgcaatcaat ccgttctact cagcgatgcg taagcacggc ggtattcgtc
    13681 acattctggc gcgtcatgtg gaaggtgctt cgcacatggc ggaaggttat acccgcgcaa
    13741 cggcagggaa tatcggcgta tgtctgggga cttccggtcc tgcgggcacg gacatgatca
    13801 ccgcgctcta ttccgcttct gctgattcca ttcctattct gtgtattacc ggccaggctc
    13861 cgcgcgcccg tctgcataaa gaagatttcc aggccgttga tattgaagca attgctaaac
    13921 cggtcagcaa aatggcggtt acagttcgtg aagcggcgct ggtgcctcgt gtgctgcaac
    13981 aggcgtttca cctgatgcgt tctggtcgcc cgggtccggt actggtggat ttaccgttcg
    14041 acgtacaggt tgcggaaatc gagtttgatc ctgatatgta cgaaccgctg ccggtctaca
    14101 aacctgctgc cagccgtatg cagatcgaaa aagctgtaga aatgttaatc caggccgaac
    14161 gtccggtgat tgttgccgga ggcggggtaa tcaatgctga cgcagctgca ctgttacaac
    14221 agtttgctga actgaccagc gttccggtga tcccaacgct gatgggctgg ggctgtatcc
    14281 cggacgatca tgaactgatg gccgggatgg tgggcctgca aaccgcgcat cgttacggta
    14341 atgcaacgtt gctggcgtcc gacatggtgt ttggtatcgg taaccgtttt gctaaccgtc
    14401 ataccggttc ggtagagaaa tacaccgaag ggcgcaaaat cgttcatatc gatattgagc
    14461 cgacgcaaat tggccgcgtg ctgtgtccgg atctcggcat tgtctctgat gctaaagcgg
    14521 cgctgacact gctggttgaa gtggcgcagg agatgcaaaa agcaggtcgt ctgccgtgtc
    14581 gtaaagagtg ggtcgccgac tgccagcagc gcaaacgcac tttgctgcgt aaaactcact
    14641 tcgacaatgt gccggtgaaa ccgcagcgcg tgtatgaaga gatgaacaaa gcctttggtc
    14701 gcgatgtttg ttatgtcacc accattggtc tgtcacaaat cgctgcggca caaatgctgc
    14761 atgtctttaa agaccgccac tggatcaact gtgggcaggc aggcccgttg ggctggacca
    14821 ttccggcggc gctgggggtt tgtgccgctg atccagagcg caatgtggtg gcgatttccg
    14881 gtgacttcga cttccagttc ctgattgaag aattagccgt tggcgcacag ttcaacattc
    14941 cgtacatcca tgtactggtc aataacgcct atctcggtct gattcgccag tcgcagcgcg
    15001 cgtttgatat ggattactgc gtacagctcg cgttcgaaaa tattaactcc agcgaagtga
    15061 acggttacgg cgtcgaccac gtaaaagtag cggaaggttt aggttgtaaa gcgattcgcg
    15121 ttttcaaacc ggaagatata gcaccagcct ttgaacaggc gaaagcctta atggcgcaat
    15181 atcgggtacc ggtagtcgtg gaagttattc tcgagcgtgt gaccaatatt tcgatgggca
    15241 gcgaactgga taatgtcatg gaatttgaag atatcgccga taacgcagtg gacgcaccga
    15301 ctgaaacctg cttcatgcac tatgaataag ggagataaat aatgttacgt ttctctgcta
    15361 atttatcgat gttatttggt gaatatgatt ttctcgcccg ttttgagaaa gctgcgcagt
    15421 gtggttttcg cggtgttgaa tttatgttcc cttatgacta cgacattgaa gaattaaaac
    15481 agatactggc gagtaataaa ctcgaacata cgctgcacaa tttaccggcg ggtgactggg
    15541 cggcggggga gcgcggtatt gcctgtattc ctggccgtga agaagagttt cgggatggcg
    15601 tagcagcagc gattcgttat gcccgtgcgc tgggtaataa aaaaattaac tgtctggtcg
    15661 gtaaaacgcc ggctggtttc agcagtgaac agattcacgc aacgcttgta gaaaacctgc
    15721 gttatgccgc gaatatgctg atgaaagaag atattttatt actgattgaa cctatcaacc
    15781 attttgatat tcctggtttc catctcaccg gaactcggca ggcgctgaaa ctgattgatg
    15841 atgttggttg ctgcaatttg aaaattcagt atgacattta tcacatgcag cggatggaag
    15901 gtgaattaac caacactatg actcaatggg ccgataaaat tggtcacctg caaattgccg
    15961 ataatccgca tcgcggcgaa ccgggaaccg gagaaattaa ttacgaatat ctctttacgg
    16021 taattgaaaa ttctgactac aacggttggg ttgggtgtga atataaaccc caaaccacca
    16081 cggaagccgg tttacgctgg atggatccgt accgttaaaa cgtaacgcta ttcagacaat
    16141 gcttttttag gtcgctaagt cggcagggga tcgtgttgtc tgaattcagg aaaagcgaaa
    16201 tttaaaagag gttaattatg aaactgggat ttattggctt aggtattatg ggtactccga
    16261 tggccattaa tctggcgcgt gccggtcatc aactgcatgt cacaaccatt ggtccggttg
    16321 ctgatgaatt actgtcactg ggtgccgtca gtgttgaaac tgcgcgccag gtaacggaag
    16381 catcggacat catttttatt atggtgccgg atacgccaca ggttgaagaa gttctgtttg
    16441 gtgaaaatgg ttgtaccaaa gcctcgttga agggcaaaac cattgttgat atgagctcca
    16501 tttccccgat cgaaactaaa cgtttcgctc gtcaggtgaa tgaactgggc ggtgattatc
    16561 tcgatgcgcc cgtctccggc ggtgaaatcg gcgcgcgtga agggacgttg tcgattatgg
    16621 ttggcggtga cgaagcggta tttgaacgtg ttaaaccgct gtttgaactg ctcggtaaaa
    16681 atatcaccct cgtgggcggt aacggcgacg gtcaaacctg caaagtagca aatcagatta
    16741 tcgtagcgct caatattgaa gcggtttctg aagccctgct atttgcttca aaagccggag
    16801 cggatccggt acgtgtgcgc caggcgctga tgggcggctt tgcttcctca cgtattctgg
    16861 aagttcacgg tgagcgcatg attaaacgca cctttaatcc aggttttaaa attgcgctgc
    16921 accagaaaga tctcaacctg gcactgcaaa gtgcgaaagc actggcgctg aacctgccaa
    16981 acaccgcgac ctgccaggaa ttgtttaata cctgtgcggc aaacggtggc agccagttgg
    17041 atcactctgc gttagtgcag gcgctggaat taatggctaa ccataaactg gcctgatacc
    17101 cgcaataaaa atggccgata tcagaaaatg aatcggccag caatattaaa aaaagaaagc
    17161 agccaaagat gttgcttcag tattaaaaat aatattttta ttttatttgt tcctcatagc
    17221 tagattaaaa agacgttatt cgatacgtga aattaagagg gatttatgga acatcagaga
    17281 aaactattcc agcaacgcgg ctatagcgaa gatctattgc cgaaaacgca aagccagcgg
    17341 acctggaaaa catttaacta ttttacctta tggatgggtt cggttcataa cgttcccaat
    17401 tatgtgatgg tcggtggctt ttttattctc ggcttgtcta cctttagtat tatgctggca
    17461 attatcctca gcgccttttt cattgccgcg gtaatggtat taaacggtgc tgcgggcagt
    17521 aaatacggcg taccgtttgc catgatcctg cgtgcttctt atggcgtacg tggcgcactg
    17581 tttcccggat tattaagagg cgggattgct gccattatgt ggtttggcct gcaatgttac
    17641 gccggatcgc tggcctgctt gatcctgatt ggcaaaatct ggccgggatt tttaactctc
    17701 ggtggtgatt tcactctgtt aggcctttct ctaccgggct taattacttt cttactcttc
    17761 tggctggtca acgttggtat aggttttggc ggtggcaaag ttttaaataa attcactgcc
    17821 attcttaacc cgtgcatcta tatcgttttc ggcggtatgg cgatttgggc gatttcgctg
    17881 gtcgggctcg gtccaatctt tgactacatt ccgagcggta ttcagaaagc agaaaacagt
    17941 ggattcttgt tcctggtggt gattaacgcg gtagttgcgg tctgggcggc accggcggtg
    18001 agcgcatccg actttacgca aaacgcccac tcgtttcgtg agcaagcgct ggggcaaacg
    18061 ctgggtttag ttgtggccta tattctgttt gcggtcgccg gggtatgtat tattgccgga
    18121 gccagtattc actacggcgc tgacacctgg aacgtgctgg atattgttca gcgttgggac
    18181 agcctgtttg cctcgttctt tgcggtactg gttattctga tgacgaccat ttccactaac
    18241 gccaccggta atattattcc ggccggttat cagattgccg ctattgcacc gacgaaactg
    18301 acctataaaa acggcgtact gattgccagt attatcagcc tggtgatctg cccgtggaaa
    18361 ttaatggaaa atcaggacag tatttatctg ttcctcgata ttatcggcgg aatgctgggt
    18421 ccggtaattg gtgtcatgat ggcgcattat tttatggtga tgcgcggaca aattaatctt
    18481 gatgaactgt ataccgcagc aggtgatttc aaatattacg ataacggctt taaccttacc
    18541 gcgttctcag taactttggt cgccgttatt ttatctctcg gtggtaaatt tattccgttc
    18601 atggagcctt tatcacgcgt ttcatggttt gtcggcgtta ttgtcgcatt cgctgcctac
    18661 gcgttattaa aaaaacgtac agcagcagaa aaaacaggag aacaaaaagt cacaggttaa
    18721 ttaatcccga tattgaacat tgagttaaaa aaccaatctg tattttacaa ggagtttgtt
    18781 atgtcttttg atttaatcat taaaaacggc accgttattt tagaaaacga agctcgcgtt
    18841 gtagatatcg ccgttaaagg cggaaaaatt gctgctatcg gtcaggatct gggcgatgca
    18901 aaagaagtga tggatgcgtc tggtctggtg gtttctccag gcatggttga tgcgcacacc
    18961 catatttctg agccgggtcg tagccactgg gaaggttatg aaacgggtac tcgcgcagcg
    19021 gcaaaaggtg gtatcaccac catgatcgaa atgccgctca accagctgcc agcaacggtt
    19081 gaccgcgctt caattgaact gaagttcgat gccgctaaag gcaagctgac tatcgatgcg
    19141 gcacaactcg gtggcctggt gtcttacaac atcgaccgtc tgcatgagct ggatgaagtg
    19201 ggcgttgtcg gcttcaaatg cttcgttgcg acctgtggcg atcgcggtat cgacaacgac
    19261 ttccgtgatg taaacgactg gcagttcttc aaaggtgcgc agaagctggg cgaactgggg
    19321 cagccggtgc tggtgcactg cgaaaacgcg ctgatctgtg acgaactggg cgaagaagcg
    19381 aagcgtgaag gtcgcgtaac tgctcatgac tatgtggctt cgcgtccggt atttaccgaa
    19441 gtggaagcga ttcgccgcgt gctgtatctg gcgaaagttg ccggttgccg tctgcacgtt
    19501 tgccatatca gcagcccgga aggcgttgaa gaagtgactc gtgcacgtca ggaaggtcag
    19561 gacgttactt gtgaatcctg cccgcattac tttgtacttg ataccgatca gttcgaagaa
    19621 atcggtactc tggcgaagtg ttcaccgccg atccgcgatc tggaaaacca gaaaggcatg
    19681 tgggaaaaac tgtttaacgg tgaaatcgac tgcctggttt ccgaccactc tccatgtccg
    19741 ccggaaatga aagccggcaa tatcatgaaa gcatggggtg gtatcgccgg tctgcaaagc
    19801 tgcatggacg tgatgttcga tgaagcggta cagaaacgcg gtatgtctct gccgatgttc
    19861 ggcaaattaa tggcgactaa cgcagcagat attttcggtc tgcagcaaaa aggccgtatc
    19921 gccccaggaa aagatgccga cttcgtcttc attcagccga atagcagcta tgttcttacc
    19981 aatgacgatc tggaatatcg ccacaaagtc agcccgtatg ttggccgtac cattggcgcg
    20041 cgtatcacga aaaccatctt acgtggtgat gtgatttacg acatcgaaca gggcttccct
    20101 gttgcgccga aaggtcaatt tatccttaaa catcagcagt aatctggccc ctgcaatgcc
    20161 cgtccttgcg gcgggcattc tccggttaag gtgtttttat gttcaatttt gcagtcagcc
    20221 gcgaaagcct gttatcagga tttcagtggt ttttctttat tttttgcaac acggttgtgg
    20281 ttcctcctac gctactttct gcttttcagt tgccgcaaag tagcctgctt acgctcacgc
    20341 aatatgcttt tcttgctacc gcactggcct gcttcgctca ggcgttctgc ggtcatcgtc
    20401 gcgctattat ggaggggccg ggcggcctgt ggtggggaac catcctgact atcacccttg
    20461 gcgaagcatc gcgcgggaca ccgatcaacg atatcgctac cagtctggcg gtgggaattg
    20521 cgctctccgg cgtgctgacg atgttgattg gttttagcgg attaggccat cgcctggcac
    20581 ggttatttac gccgtcggtg atggtcttgt ttatgttgat gctgggcgcg cagctgacca
    20641 ctatcttttt caaaggtatg ctcgggctgc cgtttggcat agccgacccg aattttaaaa
    20701 ttcagttacc gccgttcgcg ctttcggtcg cggtgatgtg cctggtactg gcgatgatta
    20761 tctttctgcc gcaacgtttt gcccgttatg gcctgctggt cggtaccata accggctggt
    20821 tgttgtggta cttttgcttt ccttcttcgc attcgctctc cggtgagttg cactggcagt
    20881 ggttcccgct cggcagtggc ggtgctttgt cgccggggat tattctgacg gcggtgatta
    20941 caggtctggt aaatatcagc aatacctacg gtgcgattcg tggcacggat gttttttatc
    21001 cgcagcaggg cgcggggaat acacgttatc gtcgcagctt tgtggcgacc ggatttatga
    21061 cactgataac cgtgccgctg gcggtaattc cattttcacc gtttgtttca tccattggtt
    21121 tattaaccca gactggcgat tacacgcggc gttcgtttat ttatggcagc gttatttgcc
    21181 tgctggtggc actggttccg gcactcacgc gactgttttg cagtatcccc ttaccgatga
    21241 gtagcgcggt catgctggtt tcttatctgc ctttactctt ttccgcgctg gtgtttagcc
    21301 agcaaatcac gtttaccgct cgcaatattt atcgactcgc attgccgtta tttgtcggca
    21361 tatttttaat ggcattaccg cctgtgtatc tgcaagacct tccattaacg cttcgtcctc
    21421 tgctcagtaa cggcttactg gtcggaattt tactggctgt tcttatggat aatcttattc
    21481 cgtgggaacg catcgaataa tttgttgaaa aaggattgat aatgaagatt gtcattgcgc
    21541 cagactcgtt taaagagagc ttaagtgcag aaaaatgttg tcaggcaatt aaagccgggt
    21601 tttcgaccct ctttcccgat gcgcactata tctgtttacc gatagcggat ggcggcgaag
    21661 ggacagtgga tgcgatggtc gccgcgacgg gcggcaacat cgtgacgctt gaagtctgcg
    21721 ggccgatggg cgaaaaagtg aatgcttttt atggcattac cggcgacggg aaaacggcgg
    21781 tgattgagat ggctgcggca agcggcctga tgctggtcgc gcctgaaaag cgtaatccgt
    21841 tgctggcctc cagttttggt acgggggagt taattcgcca tgcgctggat aacggcattc
    21901 gccatattat tctcggcatt ggtggtagcg cgacggttga cggcggtatg ggcatggcgc
    21961 aggcgctcgg tgtgcgtttc cttgatgcct acggtcaggt gctggcggct aacggcggca
    22021 atttagcacg cgtggcaagt attgagatgg atgaatgcga tccgcgtctg gcgaactgcc
    22081 atattgaagt cgcatgtgat gttaataatc cgctggtagg ggcacgcggc gcggcggcgg
    22141 tgtttggccc gcaaaaaggg gcgacgccag aaatggtcga agagctggaa caggggctgc
    22201 aaaattacgc ccgtgtttta caacagcaaa ctgaaatcaa cgtctgccag atggcgggtg
    22261 gtggcgcggc gggcggtatg ggcattgcgg cggcggtatt cctcaatgcg gatattaaac
    22321 cgggtattga gattgtgttg aatgcggtca atcttgcgca ggcagtgcag ggcgcagcac
    22381 tggtgatcac cggggaaggg cgcatcgact cgcaaacggc aggtggtaaa gcgccgctgg
    22441 gcgtggcgtc ggtggcgaag cagtttaatg tcccggtgat tgggattgct ggcgtattgg
    22501 gtgatggcgt ggaagtggtg caccagtacg gcattgacgc ggtattcagc attttgccac
    22561 gtctggcacc tctagccgaa gtgctcgcca gcggtgaaac caatctcttt aacagcgcgc
    22621 gaaatattgc ctgcgccatt aaaataggtc agggaattaa aaactgactc ttacctttaa
    22681 agcggatgcg atttatatcg catctttttt tacctgaatt gactacaaaa aaagccacgg
    22741 aataaaccgt ggcaaaatcc aacatagcta aaaataatca ggcgagtggt atgacttaaa
    22801 tctctacgtc gcggttgcaa tcttttgagt aaatataact aaacgcttca ccacgcccta
    22861 cgccataacc ggcctgtaag gagtaagcgc ccataaagat gtaatcgcct tttttcaccg
    22921 ggatccagtt attgtcgagg ttataaaccc cctgaccgga aagaatatag gcaccgtgtt
    22981 cctggacgtg agtttcgata taaccgtggc tggcacctgg cgcaaaagag aggatatgca
    23041 tgttcatatc aaaacctaac tctttgggca gaaaatccag cagaataaca tcgtccatgc
    23101 cttcataatg aatgtgttcc agttcgctgg cattgccaga aaccagccac ggcgcatgac
    23161 cttctaccgg aatatagcga cgcttatata aaaagatttg gctgtcttcg gcctgggcgt
    23221 taacaaacgt cattaaggag cctggcgggc aataaagata gccaccttcg cttaaggcaa
    23281 atgttttccc ttcggctttg gcagtgatat ttccagagat cacatacagg aacgtttcaa
    23341 tgccttcgcc accgaagccc tgttggttgc cgccgttttg atgcagtgtg accagataat
    23401 caacaaaaga ggcacccagc tttggcgtgg agaggattgt cgcgtcacaa ttttcaaagc
    23461 ccggaataat attttttacc agaccgtctg gtgttaacag tgcgaaatta ccgtgtttca
    23521 caatcgcacg gttagccagt aaatcatcgc ggtaaccggt gacgttattt aaatatccca
    23581 tttataactc cttatttctg ccaggcaagt tgataaagca tgagtgccaa cgttttgacc
    23641 ccttcggcaa ggtcggtaat attggtgcgt tccgccgggt tatggctgat cccgttgatg
    23701 ctgggaatga aaatcatgca ggtcggcacg cgaggcgcga aaatttgcgc gtcgtgcccg
    23761 gcaccactgt gcattacccg gtagttctgt ttttcacttt cacacaattc tgtcagggtg
    23821 gcgaccagct ccttattcat cggcacgggt tcttcgtcca tccataaatc gatatcaata
    23881 ccaatgtcca tttcatcgca aatcgcccgc atgtcgtttt ctaactgctg ggtgaaatcg
    23941 cgcagcacgg cagcgtcggt atgacgacaa tcaatagtga acgtggtttt acccggcact
    24001 acattcaccg tattcgggcg cggctccacc ttgccaaagg tcagcaccag cggatcgccc
    24061 atctttttcg ctttctcaac tgactgatgg caaatgcgac tgaaagcgta aaccgtatca
    24121 cgacgataac ccatcggcgt ggtgcctgca tggtttgatt cgccgttcag cgtcaccgta
    24181 tagcgacgtt gtccgacaat tgcattcacc acgccaattg actgaccatt actttccagc
    24241 acacaaccct gttcgatatg cagttcgaca aaggctttaa tatcctgacg cggagttagt
    24301 ggggcgttcg gcagagtaaa tccgcaagcc ttcattgcat cgacaaaact atttcctttg
    24361 gcatcacaga tattccgcac gtcgtcagga ttcgccagcc caaagatatt tttactgccc
    24421 cagaagacat acgggaagcg gctgccttct tcttccgcca ttgccaccac ttcgaccgtt
    24481 cgcagcggcg cgccgtattg cgttttcagc cagtcaatcg ccagccacgc cgccagcgcg
    24541 ccgaattgcc cgtcgaggtt accgccgtta accacggtat cgatatgcga accgctcaga
    24601 accacttcct gtggatattc ggtgccactc aggcgaccgt ataaattccc cacttcatcg
    24661 aaacgtgttt ccagcccgct tgctgccatt ctttttttaa attgctgctg ggtttccagc
    24721 cattccggcg aataaagtaa acgggtcatc ccacccgttg ggtcagcgcc aaaagaggaa
    24781 agccagggca gcgtttcttc tatggcttga cggaaatgtg taatcataag aaagtcctgt
    24841 ctcaataatt attgtgcaaa gggatttttc gtttcgtatg acgtgttata aagcgcgtcg
    24901 gaaattaaat actggtaaat atcatcaaca atttcgatgc cttcgacggc ggctttgcgt
    24961 tgtttaatat cctgatcctg tccgggataa taaacctgat taaaaccggg cgcgggggta
    25021 atggcattta attcgcgcat ggtctggcta agatgttgac ggaataattc gctggaggag
    25081 aaaaagttcg gattaataac gatatgtaat tgccccaaat tacgcccggc gtgtaaatcg
    25141 tcatacatcg aactaacctg tcgcccgaac ggtaagccga gtaagacgcc tgagaggacg
    25201 tcaatcatca tcatcaggcc ataccctttc ggtccggcgg cagggagcag ggcgttaact
    25261 gcaaatggat cggttgttgg tgcaccgttt ttatcgaccg cccaggtatc cgggatagac
    25321 atattacgcg agcgggcgtc gagcactttt ccccatgcct gtacggtagt cgccatatca
    25381 aaggtaagga tctcgtcgcc ttctcccggc gcggcaaagg ccagtgggtt agtaccgtag
    25441 taaatttccg cgccgccaaa cggcaccacc attggatcgg actggcacat cgaaatgcca
    25501 attaacccgg cgcgagctgc ctgctgtaca aaataagaga ttgcgccgct gtgacccatc
    25561 cggctgatac cgaccaccgc aacgcctttt tgctgggcgg ttttgatggc atgttccata
    25621 cccattttcg ccgcgacctg tccggcagca ttgtcggcat gtaaaattgc cgagcacggt
    25681 ccggtttcct caagacgaaa ctccggttcg cggttggtgc cgccttttga aatgcgttcg
    25741 gcgtagtatt caacgcgcac cgcgccatga gagtggatcc ctctggcatc ggcgtaaacc
    25801 aatacttcag ccacggttgc agcgtgctca cgttttaacc cagcctggca gagtttattc
    25861 tcgattagct ggtggagtgt ttcccgactg attttcatct gtcttccttt ttaacgacgg
    25921 tgtgaagcat gactgcaatt aacatacagg gaaaatatct ggattatgtg atccagacag
    25981 gcaaaaaaat atagttagaa tttatttgat aatccgctcg cttttaacct ggtttttaaa
    26041 acaaccccac ttattaaaaa ataatacgta atagcctggc ggttatttga attcttttgt
    26101 taataatccc tgtgtgatat tcatcacctt atttactcgt tgtcatcgat accgtaatcg
    26161 ccatattaac actgctcgtg caattgccat gggtgcaatt tttaaggagt tgttatgatc
    26221 cacgccttta ttaaaaaagg gtgttttcag gattcggtca gtttaatgat tatttcacga
    26281 aaactcagcg aatcagaaaa tgttgatgat gtttccgtaa tgatgggtac gcccgccaat
    26341 aaagcgttat tagatactac aggtttctgg catgacgatt ttaatcacgc cacgccgaac
    26401 gatatttgcg tggcaattcg tagcgaagcg gcggatgcgg ggatcgcgca ggcggttatg
    26461 cagcagcttg aagaggcgct aaaacaactg gcgcagggtt caggcagcag ccaggcgttg
    26521 acgcaggtgc gtcgctggga cagtgcctgt cagaaattac ccgatgccaa tctggcgctg
    26581 atttcagtgg ctggtgagta tgcggcggag ctggcgaacc aggcgctgga tcgcaacctc
    26641 aacgtgatga tgttctccga taacgtcacg ctggaagatg aaatccaact taaaacccgc
    26701 gcgcgggaaa aaggcttgct ggtgatgggg ccagactgcg gtacgtcgat gattgccgcc
    26761 acgccgctgg cttttgctaa cgtgatgccg gaaggcaaca ttggcgtcat aggcgcttcc
    26821 ggtaccggga ttcaggagct gtgctcacag attgcgctgg caggggaggg aattactcac
    26881 gcgattggtc ttggcgggcg cgatctcagc cgtgaagtgg gcggcatcag tgcgctaacg
    26941 gcgctggaaa tgctcagtgc agacgagaaa agcgaagtgc tggcatttgt ttcaaaacca
    27001 cctgccgaaa ctgtgcgtct gaaaattgtt aatgccatga aagcaactgg caaaccgacg
    27061 gtggcgctgt ttttaggtta taccccggcg gtggcccgcg acgagaatgt ctggtttgcc
    27121 tcctcgctgg atgaggccgc tcgtctggct tgtctgcttt cacgcgtcac tgcgcgacgt
    27181 aacgcgatag cgcctgtcag cagcggattt atttgcggtt tgtataccgg cggtacgctg
    27241 gctgctgaag cggcgggatt acttgccgga caccttggcg tggaagccga cgatacccat
    27301 caacatggca tgatgctgga cgccgacgga catcagatcc tcgacctggg cgatgatttt
    27361 tacaccgtcg ggcgtcccca tccgatgatc gacccgacct tacgcaacct gttaattgcc
    27421 gatctcggcg ctaaaccgca agtgcgcgtg ttgctgcttg atgtcgtgat tggcttcggt
    27481 gcgaccgccg atcctgccgc ctcgctggtg agcgcctggc aaaaagcctg tgccgcgcgt
    27541 tcagacaatc aaccactgta tgccattgcc acggtgacag gcactgaacg tgacccgcaa
    27601 tgccgctcgc agcaaatcgc cacgctggaa gatgcgggga tcgcggtagt gagttcgcta
    27661 ccggaagcca ccttgctggc tgcggcgtta attcatccgc tctcgtcagc cacacagcaa
    27721 cacacaccgt cattactgga aaacgtcgcc gtgattaaca tcggattacg cagctttgcg
    27781 ctggagctac aaagcgccag caaaccggtt gtgcattacc aatggtcgcc agtcgccggc
    27841 ggcaataaaa aactggctcg tttattagaa cgtttgcaat aaggggttcc catgtttaca
    27901 tcagtggcgc aagccaatgc tgcggttatc gaacaaattc gtcgcgctcg tccacactgg
    27961 ctggatgtgc aaccggcttc ttcacttatc agcgaactaa acaagggcaa aacactgctt
    28021 cacgccgggc cgccaatgcg ctggcaggag atgaccgggc caatgaaagg ggcgtgcgtg
    28081 ggcgcatgtc tgttcgaagg ctgggcgaaa gatgaagcgc aggcacaggc aatgctggag
    28141 cagggggaag tgaacttcat tccttgtcac catgtgaatg ctgtcgggcc aatgggcggc
    28201 attacttctg ccagtatgcc gatgctggtg gtcgagaacg tgaccgacgg caaccgggcg
    28261 tactgcaacc tcaacgaagg tatcggcaaa gtgatgcgtt ttggcgctta cggcgaagat
    28321 gttctgactc gccatcgctg gatgcgcgat gtgttaatgc cagtattaag cgcggcgctg
    28381 gggcgcatgg agcgcggtat cgatctcacc gcgatgatgg cgcagggcat taccatgggc
    28441 gatgagttcc atcaacgcaa tattgcttcc tcagcactgt taatgcgtgc gctggcccca
    28501 cacattgctc gcctcgatca tgataaacag cacatcgcgg aagtgatgga tttcctcagc
    28561 gtgaccgatc agttcttcct taacctcgcg atggcttact gcaaggcggc gatggatgct
    28621 ggtgcgatga tccgcgcagg cagcatcgtc acggctatga cccgcaacgg caatatgttc
    28681 gggattcggg taagcgggct gggcgaacgc tggtttactg cgcctgtaaa cactccccaa
    28741 gggctgtttt tcaccggctt ctcgcaggag caggcgaacc cggatatggg cgatagcgca
    28801 attaccgaaa cctttggtat tggcggtgcg gcgatgatcg cagcgcctgg cgtaacgcgc
    28861 tttgtcggtg cgggtggcat ggaagcggcc agagcggtat ctgaagagat ggcggaaatc
    28921 ttccttgaac gcaatatgca gttgcagatc cccggctggg atttccaggg cgcgtgcctg
    28981 gggctggata ttcgtcgcgt ggtagaaacc ggcattacgc cgctcatcaa taccggaatc
    29041 gcccataaag aggcggggat cgggcagatt ggcgcaggca ctgtgcgggc accgttggcc
    29101 tgctttgaac aagcgctgga agcactggct gaaagcatgg ggataggttg aggaacgcgc
    29161 aatgacgatc atccatcctc tgcttgccag ccgtagcgca ccgaattatc gccagtcctg
    29221 gcggttagcg ggcgtgtggc ggcgggcgat taacctgatg acggaaagcg gcgaactgtt
    29281 aacgctgcat cgtcagggta gtggtttcgg ccccggagga tgggtgcttc gccgcgcgca
    29341 attcgatgcg ttatgcggtg gattatgcgg caatgaacga ccacaggttg tggttcaagg
    29401 gattcgcctc gggcgtttca cggttaaaca gccacagcgt tattgtttgc tgcgtattac
    29461 gccgcctgcg catcctcaac cgcttgccgt tgcatggatg caacgcgcgg aggaaaccgg
    29521 gcttttcggg ccactggcgt tggcggcagg cgatccgctt cctgctgaat tacgccagtt
    29581 tcgtcactgt tttcaggccg cgctcaatgg cgttaagacc gactggcggc actggctggg
    29641 taaaggccca ggattaacgc caagtcatga tgacacgctg agcggaatgc tgctggcggc
    29701 ctggtattat ggcgctttag atgcgcgctc cggtcgcccg ttttttgcct gttccgacaa
    29761 tcttcaactc gttaccacag cggtgagcgt cagttattta cgttatgccg cgcaaggata
    29821 tttcgcctcg ccactcctgc actttattca tgctctgagt tgcccgaaac gtaccgctgt
    29881 tgcgattgat tcgctgctgg cgctggggca tacgtcaggg gcagatacgc tgttggggtt
    29941 ctggcttggc caacaattat tacaaggaaa atcatgaaaa cactggttgt ggctcttggg
    30001 ggcaacgcct tactccagcg cggtgaggcg ctgacggcag aaaatcaata tcgcaacatt
    30061 gccagtgctg tacccgcact ggcgcgcctg gcccgttctt atcggctggc gattgttcac
    30121 ggcaacgggc cacaggtggg gctgctggcg ttacagaatc tggcgtggaa agaggtagaa
    30181 ccgtatccgc tggatgtgct ggtagcggaa agccagggga tgattggcta tatgctggcg
    30241 cagagcttga gcgcacagcc gcagatgccg cccgtgacga cggtgctgac gcgcattgag
    30301 gtttcgcctg atgatccggc gtttttgcag ccagagaagt ttattggtcc ggtttatcag
    30361 ccagaagaac aagaggcact ggaagcggct tacggctggc agatgaaacg tgatggtaaa
    30421 tatttacgcc gggtggtggc atctccgcaa ccgcgtaaaa ttctcgacag cgaagccatc
    30481 gagttgttgc tcaaagaggg gcatgtggtg atttgcagtg gcggcggcgg tgtgcctgtg
    30541 acggaagacg gggcagggag tgaagcggtg attgataaag atcttgccgc tgcgttgctc
    30601 gccgagcaga ttaatgccga tggactggtg atcctcaccg atgctgatgc ggtatatgaa
    30661 aactggggaa cgccgcagca acgtgccatt cgccatgcca caccggatga gttagcgcca
    30721 tttgccaaag ccgatggttc gatggggccg aaggtaacgg cggtgagtgg ctatgtcaga
    30781 agccgtggta aacccgcgtg gattggggcg ttatcgcgga ttgaagagac gctggcgggc
    30841 gaagcgggga cttgtatttc gctgtagtga tgggcaccag gcaattggca catccagcct
    30901 gatgcgatgc tggcgcgtct tatcaggcct acagtcggtg ttggagcggt
//
LOCUS       DAPHCV010000055        30857 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.055, whole genome shotgun sequence.
ACCESSION   DAPHCV010000055 DAPHCV010000000
VERSION     DAPHCV010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.055"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(82..2529)
                     /gene="glgP"
                     /locus_tag="RH033_002472"
     CDS             complement(82..2529)
                     /gene="glgP"
                     /locus_tag="RH033_002472"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HDV2991647.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNDLQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYERQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2548..3981)
                     /gene="glgA"
                     /locus_tag="RH033_002473"
     CDS             complement(2548..3981)
                     /gene="glgA"
                     /locus_tag="RH033_002473"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDV2991648.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3981..5276)
                     /gene="glgC"
                     /locus_tag="RH033_002474"
     CDS             complement(3981..5276)
                     /gene="glgC"
                     /locus_tag="RH033_002474"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDV2991649.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5294..7267)
                     /gene="glgX"
                     /locus_tag="RH033_002475"
     CDS             complement(5294..7267)
                     /gene="glgX"
                     /locus_tag="RH033_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HDV2991650.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCIFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(7264..9450)
                     /gene="glgB"
                     /locus_tag="RH033_002476"
     CDS             complement(7264..9450)
                     /gene="glgB"
                     /locus_tag="RH033_002476"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HDV2991651.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9723..10826)
                     /gene="asd"
                     /locus_tag="RH033_002477"
     CDS             complement(9723..10826)
                     /gene="asd"
                     /locus_tag="RH033_002477"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDV2991652.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            11018..11611
                     /gene="yhgN"
                     /locus_tag="RH033_002478"
     CDS             11018..11611
                     /gene="yhgN"
                     /locus_tag="RH033_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HDV2991653.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11668..13008)
                     /gene="gntU"
                     /locus_tag="RH033_002479"
     CDS             complement(11668..13008)
                     /gene="gntU"
                     /locus_tag="RH033_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HDV2991654.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVNSGVGPALGEALT
                     GMGLPIAITCFVLAGAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(13012..13539)
                     /gene="gntK"
                     /locus_tag="RH033_002480"
     CDS             complement(13012..13539)
                     /gene="gntK"
                     /locus_tag="RH033_002480"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDV2991655.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RCNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLRAGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13678..14673)
                     /gene="gntR"
                     /locus_tag="RH033_002481"
     CDS             complement(13678..14673)
                     /gene="gntR"
                     /locus_tag="RH033_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HDV2991656.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14897..15592)
                     /gene="yhhW"
                     /locus_tag="RH033_002482"
     CDS             complement(14897..15592)
                     /gene="yhhW"
                     /locus_tag="RH033_002482"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HDV2991657.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVYQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15715..16752)
                     /locus_tag="RH033_002483"
     CDS             complement(15715..16752)
                     /locus_tag="RH033_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDV2991658.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTI
                     AQAKELFALAKSKGLIVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDTLYQTITNGAPNYVKESEVLTNLEILERGFE
                     QASPSAVTLAR"
     gene            17086..17574
                     /gene="yhhY"
                     /locus_tag="RH033_002484"
     CDS             17086..17574
                     /gene="yhhY"
                     /locus_tag="RH033_002484"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV2991659.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQLPHPSEGWWPER
                     LKDRQGFKQLVASIDGIVVGHLFIEAYQGPRRSHVATFGICVDSRWHNRGVASALIRE
                     LIEMCDNWLRIDRIELTVFADNAPAIAVYKKFGFHIEGTGKNYAFRNGEYVDAYYMAR
                     VK"
     gene            complement(17634..19379)
                     /gene="ggt"
                     /locus_tag="RH033_002485"
     CDS             complement(17634..19379)
                     /gene="ggt"
                     /locus_tag="RH033_002485"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDV2991660.1"
                     /translation="MIKPTFLRRVAIAALFTGSCFSTVAAPPAPSPVSYGVEEDVFHP
                     VRAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLI
                     RSKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALD
                     KYGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGAPLK
                     KGDKLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVER
                     TPISGEYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYA
                     YADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQT
                     THYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGG
                     DANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAE
                     ATNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELY
                     GASDPRSVDDLTAGY"
     gene            19499..19939
                     /locus_tag="RH033_002486"
     CDS             19499..19939
                     /locus_tag="RH033_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HDV2991661.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19926..20669)
                     /gene="ugpQ"
                     /locus_tag="RH033_002487"
     CDS             complement(19926..20669)
                     /gene="ugpQ"
                     /locus_tag="RH033_002487"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDV2991662.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGARYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKTVALAARELWTGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20666..21736)
                     /gene="ugpC"
                     /locus_tag="RH033_002488"
     CDS             complement(20666..21736)
                     /gene="ugpC"
                     /locus_tag="RH033_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HDV2991663.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTTGDIWIDRKRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVREPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLEGRVNNEGTHFVLDGGIALPLNGGYRQYAGRKM
                     TLGIRPEHITLRSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGG
                     TLWLHLPENQLHLFDGETGQRV"
     gene            complement(21738..22583)
                     /gene="ugpE"
                     /locus_tag="RH033_002489"
     CDS             complement(21738..22583)
                     /gene="ugpE"
                     /locus_tag="RH033_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HDV2991664.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22580..23467)
                     /gene="ugpA"
                     /locus_tag="RH033_002490"
     CDS             complement(22580..23467)
                     /gene="ugpA"
                     /locus_tag="RH033_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HDV2991665.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23643..24959)
                     /gene="ugpB"
                     /locus_tag="RH033_002491"
     CDS             complement(23643..24959)
                     /gene="ugpB"
                     /locus_tag="RH033_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HDV2991666.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEQSTKS"
     gene            complement(25168..25545)
                     /locus_tag="RH033_002492"
     CDS             complement(25168..25545)
                     /locus_tag="RH033_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDV2991667.1"
                     /translation="MTIQFISAEEIIYFHDKLLSVTPGVTGMSDPGRAEALLYRVLNK
                     YEYEGVSDLWLLAAMHLLAISRGHIFNDGNKRTALFITLLFLKRNGIILAANPEFVEL
                     TVDVAAGKVSLEDIVTRLRQRGT"
     gene            complement(25542..25763)
                     /locus_tag="RH033_002493"
     CDS             complement(25542..25763)
                     /locus_tag="RH033_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDV2991668.1"
                     /translation="MHTMNYSEARQNLALVLETAATGTPVTITRRGHKAAVIISAEEF
                     ERYQKAKLDAEFDAIMAIHGSEIKELADK"
     gene            complement(25851..26564)
                     /gene="livF"
                     /locus_tag="RH033_002494"
     CDS             complement(25851..26564)
                     /gene="livF"
                     /locus_tag="RH033_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HDV2991669.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(26566..27333)
                     /gene="livG"
                     /locus_tag="RH033_002495"
     CDS             complement(26566..27333)
                     /gene="livG"
                     /locus_tag="RH033_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HDV2991670.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(27330..28607)
                     /gene="livM"
                     /locus_tag="RH033_002496"
     CDS             complement(27330..28607)
                     /gene="livM"
                     /locus_tag="RH033_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HDV2991671.1"
                     /translation="MKPMHIAMALLSAVMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(28604..29530)
                     /gene="livH"
                     /locus_tag="RH033_002497"
     CDS             complement(28604..29530)
                     /gene="livH"
                     /locus_tag="RH033_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HDV2991672.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTSWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(29578..30687)
                     /gene="livK"
                     /locus_tag="RH033_002498"
     CDS             complement(29578..30687)
                     /gene="livK"
                     /locus_tag="RH033_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HDV2991673.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQYIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLITQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANKGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
BASE COUNT         7470 a         8608 c         7742 g         7037 t
ORIGIN      
        1 ttgtttattg tgtatagaaa taccgtgagt tccactctgt attacttcag cggaacacac
       61 ggtatttagc catatttgaa cttacaatct caccggatca atatgccaga tatgatcggc
      121 gtactctttg atcgtacggt cagaagagaa gtagcccata ttggcaatgt tcagcatcgc
      181 ttttgcggtc cactcttcct gacgctcgta gagttcgtcg actttatcct gacaatcgac
      241 atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca gcgaatcgac
      301 cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga tttgcgtcag
      361 cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc cctgacgacg
      421 cagttcttcc acttcttccg ctgtgttacc aaagataaag atattgtcag caccgacatg
      481 atccagcatc tcgacattcg caccgtccag cgtaccgata gtcagcgcgc cgttaagcgc
      541 aaacttcatg ttactggtgc cggaagcttc cgtccccgcc agcgaaatct gttcagacag
      601 atcggccgcc ggaatgatca actgcgccag gctaacactg tagttcggga tgaacacgac
      661 tttcagcttg tcgccaatct gcggatcgtt gttgatcact ttcgctacgt cattgatcaa
      721 atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccaccaa aaatattcac
      781 gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg tgatcacgtg
      841 caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgca catcgaacag
      901 cgctttcgga tttaccacca cattcagctg ctgggcgata tactccgcca gacgcttttt
      961 gttctccagc ttcgcctgat gcaccgcgtg attaaccatt gggaaatcac agtgttgttg
     1021 cagatcatta agcaggctaa ggtcggtgcg ccagttgcgg cccaggtgtt cgtccaacac
     1081 ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac cgttggtgac
     1141 gttggtgaaa cgacccggga agattttcgc aaagtcggca aatagcgatt gcaccatcag
     1201 gttagagtgc agctccgata caccgttaac tttgtggctc accacaaccg ccagccaggc
     1261 catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca gcagatcggt
     1321 atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt caaagatgat
     1381 ctgcaggtga cgcggcagaa ttttacccag catatcgact ggccaggttt ccagcgcctc
     1441 gctcattagc gtgtggttgg tgtaggagaa gacctgacaa cacacttcaa acgcgtcgtc
     1501 ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg acagcaccgg
     1561 atgagtatcg ttgaggtgaa tcgcaatttt atccgccagg ttatcgtagg ttttatgcaa
     1621 ctgataatgg cggcttaaaa tgtcctgaat ggtcgaggaa accaggaagt attcctgacg
     1681 cagtcgcagc tcacgcccgg agtaggtgga gtcgtccgga tacagtacgc gagatacgtt
     1741 ctcggagtgg tttttatctt ccactgccgc gaagtagtcg ccctggttga atttaccgag
     1801 gttaatttcg ctactggctt gggcactcca caaacgcaac gtgttggtcg cgtcggtgtc
     1861 gtaacctggg attatctgat cgtaagctac tcccagaatc tcttcggttt caatccagcg
     1921 cgttttttta ccttcctgct gaatgcgacc gccaaaacgg actttatagc gcgtgttgtg
     1981 gcgtttgaat tcccacgggt taccgtattc cagccagtag tcaggcgact ctttctggct
     2041 accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt aaccgcgccc
     2101 cggcaaccct aacgtcgcca gagaatcgag gaagcaagcc gccagacggc ccaggccacc
     2161 gttaccgagg cccgggtcat tttcttcatc aatcagctct tcgagattta atcccatcgc
     2221 ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt tggaaagcgt
     2281 acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt gcgacaactg
     2341 ggcacggttt gagcgtaacc agcgctccac gagacgatcg cgcacagcaa ataacgttgc
     2401 gttcagccat tcatgtttat tggcgacgac cggatccttg ccaatcgtaa acatcagctt
     2461 gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat atgtaaacgg
     2521 agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct cacggtacga
     2581 cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca caaaccgcca
     2641 cagtgaagga cgggaccaca gtacaaaagc acgtcgaata gcccgtaaca gcgaccaggc
     2701 attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct caagagaaca
     2761 gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg taccgtactt
     2821 caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca gaatgacgtc
     2881 cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa cgcccacctg
     2941 gccggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat cgcccgcgcc
     3001 gagtagcgcc agctgcccgc cctgctccag aagaccaggt aaggcttcca gcaccaggtc
     3061 gagacctttc tggctagtca gacggctcac cactgcaaaa agcggcactt tatcgtcaac
     3121 cttaagcccc attgcgattt gtaactggcg cttattttcc gctttatctt ccaacgtatc
     3181 gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt tctcgtccac
     3241 gccgttcagt acgccggaaa gacgcccttc acggtgacgc tgttgcaaca gaccttccat
     3301 accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttgggctga ctgccgtaat
     3361 gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga attccagccc
     3421 atgaatatta aagaacgacc atggcaattg gatgtcattc atgtgatgtg cataaaacat
     3481 gccttgatag gccaggttgt gcacagtaaa caccgacttc gccggacgcc cgcgcgccgc
     3541 cagatacgca ggcgcaaggc ctgcatgcca gtcgtgcgca tgcaccacat caggacgcca
     3601 gaatgggtca agcccgctgg ccatttccgc cccaacccac cccagcagcg caaaacgcaa
     3661 tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg gacgatcata
     3721 gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga acaacagcgt
     3781 gatatgtccg gcgaaggtat cacgacggga tactacctgc gcatcggtca cgccacggcg
     3841 aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga tttgtgctgc
     3901 gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga acatctctga
     3961 acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg tagcatttcg
     4021 cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc ttcctctgcg
     4081 ttttcaccaa tcaccatgcc ttccggaata acacaagcac gatcgatgac gcagcggcgc
     4141 agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc aatgttgcag
     4201 aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga accggagatc
     4261 acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga gcgatcctgc
     4321 acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt gcgatcgtac
     4381 atatccagtt ccggcaccac agaggccaga tcgaggttcg ctttccagta agcttccagc
     4441 gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca agagagcggg
     4501 aacgggtgcg cataagccag accggcttcg gtgatcttgg gaatcaaatc tttgccaaag
     4561 tcgtggctgg agttctcatc gcgatcgtct tcttccagca gttcatacag atagtcggcg
     4621 tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg cattgacggt
     4681 gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac cgccataacg
     4741 ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca acgtgcgcct
     4801 ttttcgacgt ggtcgataag catacgcgag tagtcttgct tgtagatatg gtcgcccgcc
     4861 aggatcacca cgtattccgc tttataacgg cggataatat cgaggttttg ggtaactgca
     4921 tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg cagcagatcg
     4981 acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg ctgcaccaga
     5041 gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccggagtt gatgcagtta
     5101 gacagcgcaa agtcgataat gcggaactta ccgccgaagt gtacggccgg ttttgctcgc
     5161 ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag ggcaacagat
     5221 ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact aaccatgact
     5281 aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc tgccagacag
     5341 ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac tccccagcag
     5401 gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag cgatccgaga
     5461 gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa ggttgagcat
     5521 atcgatttag ccaacggaca ttgccgtcgc cttcttccca ccagcgattc tccaccaaag
     5581 cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa ccactgcttg
     5641 cctgcgacca gtccaaccag gttaattggt tatcctgaca gtaggcattg ttattgccat
     5701 gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtaccctgg gagagcaaca
     5761 acgttgttaa cagggcgtga atgctgtcgc gacgccgttc aaccaggtca agagtaccgc
     5821 ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg cgattttctt
     5881 ctccgtttgc ttcattatgt ttatggttga agcaaacgca gtcgcgaagc gtaaaaccgt
     5941 catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt ttaaaaacat
     6001 cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa tgcagccaga
     6061 aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc gggaaatttc
     6121 ccacctgata accaccagga gcaatatccc acggttcagc aattaacttc acctgcgaga
     6181 gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac tctggcgtac
     6241 ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt tctacccaat
     6301 aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg agcgtgttgc
     6361 cacaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag ctacggttgt
     6421 cgatcccacg cagagagaat aacgggccgt cgaggtccag ttccgcacta tggttgagca
     6481 cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca tcgcgaaact
     6541 catccagcgc cgtttctggg gagcaggcat acgccggatg cagcgcaaac atcgccaccg
     6601 ggttataacc ccagtagtta cttagaccca tacgttgcag gcgtggttcg ctggcaaact
     6661 gcgccaccgg cagcagttcc agcgcggtaa tgcccaactg tttcaaatag ttgatcatca
     6721 ccggatgccc cagggcttta taagtgccac ggatctcgac cgggatctcc gggtgcaggt
     6781 acgttaatcc tttgacatgg gcttcataaa tgatggtact gccccacggc gtgcgcggcg
     6841 gagcatcatc ctcccagtca tagtgatcaa ccaccactac gcatttcggc gcaatggcgg
     6901 cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta tctttaaatt
     6961 ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta aagcgatgcc
     7021 cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc ggacgcgcat
     7081 ccggcagata accgtgccaa atgtcgccac tgtgccctgg caagtcatag cgatgttcct
     7141 ggccattggc gtcaaagata cacagttcta cccgctcggc atgagcggag aaaagtgtga
     7201 agttgacgcc ctgaccgtcg taatgcgcgc cgaggggtgc gggtttgcca atggcgagtt
     7261 gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagtg tcaggcttag
     7321 tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc cattgccggc
     7381 attactgccg tgatagtgca tggagtcggt attgaggatt tcacgccatt tgcccggctg
     7441 gttaatgccg aagcgataat catgacgcgg caccggagta aagttactgg caacgatgat
     7501 ttcattaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt tgtcatccac
     7561 caccagccat tcaaagccgt acgggtcaaa atccagttca tgcatcgctt tatggtggcg
     7621 gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt tatcgccgcc
     7681 ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct gggcaaattc
     7741 gttccccatg aacagcagtt tcttgcccgg gaatgcccac atccagccat agtaggcgcg
     7801 caggttagcg aatttctgcc atgcgtcgcc cggcatccgg tcgagaatcg attttttacc
     7861 gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt tgtagagcat
     7921 cccgaaggtc agtttatcgt gatgatactg acgataaacc gggtcgagct tcatgtagtc
     7981 cagggtgtca tgcatccagc ctaggttcca cttgtaccag aagcccagac cgcccatgtc
     8041 ctgcggacga gaaacgccag ggaaatcggt agactcctcc gccattgtca ccgcaccgga
     8101 aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt caagattctc
     8161 gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt cgcgataaat
     8221 cattgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcga tccagtaaag
     8281 cgcgttaccg acaaggaagt tactgacttc acggcgacca tagttgtaga tcagcgtgtt
     8341 ccagtcctga tgatagcctt cgcgcggatc gctgtgttca tacaagttcg tgccatcaaa
     8401 ttcggcaagc gcaaagtcat cggtcgggaa gtggcctggc acccagtcga gaatcacgtt
     8461 cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcggg taccaaaacg
     8521 gcgggtcggt gcatacaggc cggttggctg ataaccccaa ctgccatcga acggatgctc
     8581 gttaatgggc agcagttcga ggtgggtaaa gcccatccat ttagcataag gcaccagttg
     8641 atcggccagc tcgcggtagc ttaaccagaa attgttgtcg gtgtggcgac gccaggagcc
     8701 caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt tgcgctcttc
     8761 agtctgcaca accttttccg gcagcccgca aataagagac gcggtttccg ggcgcatttg
     8821 cgcttcgaag gcataagggt cggacttcag gcgcaagttg ccattggcat caatcatctc
     8881 atatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga tgccgctctc
     8941 tttacgcagg cgcatcggat gacggcgacc gtcccagtag ttgaattgcc caaccaccga
     9001 gacccgacgg gcgtttggag cccagacaga aaaacgcgta cctgtgacgc catccatagt
     9061 atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag ataatagcca
     9121 ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatctatca ggttttgctg
     9181 accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg gaatgacacc
     9241 gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc cggttttcgg
     9301 ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca gtcccgcggt
     9361 ggttttatgc atccccagta cggaaaaagg atccgcaaaa tggcctgcaa ttagcgcgtt
     9421 aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt gtgtcacccc
     9481 atccaactga atttttgact tctggttgtg acattttttt gacctgaacg gcgcagcact
     9541 ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact catgtattca
     9601 gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg aaacatctgc
     9661 gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt aatgaataaa
     9721 gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca cagcagctgg
     9781 tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg caggcggcct
     9841 accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat agtgatttcc
     9901 cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc ttccacggtc
     9961 ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca gcgcaatgcc
    10021 ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag gatcttgttg
    10081 gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcaagctg tttgtcgatc
    10141 cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag ctcaccgcta
    10201 cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg ggtcgcgagt
    10261 tcatctgcca catggccata cagatggccc atctgggtta ataactcacg catatgtcgc
    10321 gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac aagatcattg
    10381 gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc gccaacaaaa
    10441 gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg gtcaagaatg
    10501 atgatggcgt catctttcat gcgcagagat gatgctgcgt caatccagta accttgccat
    10561 ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg acaggtcaca
    10621 atgatatcga gggcctttag cgcctccaga tcaaaggcat cctgaagtgt gccagtggtt
    10681 ccgccaaaag acggcgcagc ctgaccaagc tgagaagtag aaaagaagac agggcgaatg
    10741 gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac cataccgcgc
    10801 cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt gtgctgtata
    10861 aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagcagcca aagtcgcaag
    10921 tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct ttgttagcac
    10981 gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca tttctgcagc
    11041 agttttattg atcctgatta tggatccgct cggaaaccta cctattttca tgtccgtact
    11101 gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc ttattgctct
    11161 cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc ttagcctgcg
    11221 ggcagaaacc gtctccattt ctggcggcat cattctgttt ctgatcgcca ttaagatgat
    11281 tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc cgtttatcgt
    11341 gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga tgttgttgtc
    11401 tcatcagtac ccgaatcaga tgggacatct ggtaattgct ctgctgctgg cctggggtgg
    11461 cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg agaaaggggt
    11521 gaatgcactt gaacgcctga tgggattgat tctggtgatg atggcaaccc agatgttcct
    11581 cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa agagcccggt
    11641 agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaaagcaa tcatcccaac
    11701 gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca gcgtttcggc
    11761 ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa cgtggctgac
    11821 aacaatcgaa ccaccggcga tacaaatcga cagcgccgcc atttgcgcac cggagtagtt
    11881 cagttgttca ataaccggca ttaccagtcc caccgccgtt aaacaggcaa cggtggcaga
    11941 accctgaatg atacgcaccg ccccagccag cacaaagcag gtgatggcaa tcggcaggcc
    12001 catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagttaacca gcacctgttt
    12061 gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca gcgcgtgacc
    12121 gcaaatttcc atcactttat ctttcggcat accctgacgc atcgccaggc cgtaaatcgc
    12181 caccagacaa gcaaccagaa tcgcggtaaa cggatggcct ataaactcaa accattcgta
    12241 agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca ccagcaccag
    12301 tggcaacagg atcagcgaca ggctgaatcc gaaggatggc attttgcctt cgccgagatg
    12361 cggttcgctg atgtcgtcag gaatatgcaa ctccacgtag cggctgatga aattacccca
    12421 cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca ggatcatcca
    12481 gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggtccag gcaccaggaa
    12541 cgcagccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca ggttcgtacc
    12601 ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca cttcaaagaa
    12661 cagcggtagc gcacacacca gccccgcaag gccgatggca taatgcgcgc ggctgtgacc
    12721 gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat gtaagatctt
    12781 gccaaacata gcccccaggg cgacaaccac cgccaggaag ccgagggtgc ctcccatccc
    12841 tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc cagcccccat
    12901 ggacaccacc attaaagcca ggaaagcgtg catacgcgcc ttcatgacta aaaacagcag
    12961 cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta cttatttgcc
    13021 ttttttaatg acctcaatgg tgcttgccac aacaccttcc agcggttgat cgatatccac
    13081 caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact gcgtcaccaa
    13141 catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cggctttcaa tcacatcaaa
    13201 atcgcctttc aggtagatga aagagagatt cggattaccc gcgcgtagca agtcgcgata
    13261 gtgttttttc agtgcagaac agacgatcag cgacacttta ttggtgcgct gcatagcaaa
    13321 cgcggcgtcg ttcagcgcct gcaaccacgg tttgcgatcg tcgtcattca gcggttcacc
    13381 agacgccatt ttttcgatat tgcagcgcgg atggaggaaa tcgccatcaa gaaacgcggc
    13441 atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg atacgcccat
    13501 caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc cacaatacaa
    13561 gaattaaaaa tgttacgggt aactgttatc ggtaacattg tccggccgga caatatcaga
    13621 agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt tgtgggctta
    13681 aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg tcacagattc
    13741 gccacgaata cgcgccagca ggcgttcagc gccgatactg cccatccgct cacgcggcgt
    13801 cagtacgctc gccaaccgtg gttccatcac ctgaccaatg tcatgaccgt ggaaaccggc
    13861 aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg ccgcgccgac
    13921 cgccaggtca tcgttggtac agaacacgcc atccagctgc ggatattccc gccgcgcctg
    13981 gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca cgctatatgg
    14041 caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga tagtacgttc
    14101 gtcgagacgt gcgccgagat aggcaatgtg gcgatgcccg cgagcaataa tggcggtggt
    14161 catctggcgt gccgcttcaa agttatcaaa accgacggca atatcgaggc acggcgactt
    14221 gctgtccatc aactccacca ccggaatccc cgccacttca atcatcttta aggtgcgcgg
    14281 cgtgtgggta cgttcagtga ggatcagtcc gtcgatattc caggagagca ttgattcgag
    14341 acgttcttgc tccatttccg gtttataacc gtagtgggcc agcatggtct gataaccgtg
    14401 cgcgtcggtg acgctttcga ttccgcgtaa tacttccgca aaaacctggt tggtgagaga
    14461 aggtaacagg acgccaatcg cccggctggt ggcgttagag aggatatcgg gtgcgcgatt
    14521 gggaatataa cccagttcat caagagcggc ggcaatcttg ccgcgtagag cgacggaaac
    14581 ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta cacggtcagc
    14641 cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa agagatagat
    14701 tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc ttgtttacaa
    14761 ttattagata tacactaaat caattgcatc gcattgtgct aacgacacgg caaaaccccg
    14821 gaagcgtaca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag gcaggcttca
    14881 aaaatgccgt tgagctttaa accggcggca gatcgaacag taacacttcg ctgtcgctat
    14941 ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg gtcgaggctt
    15001 tcacgccgtt aatggtgaca ttgcctttca ccacctggat ccagacgcgg cgttcagcgg
    15061 caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt tccatatcct
    15121 gatacacttt cagcgaacca tcacgcgcat ccggcgagag caccagttgt ttgccctgca
    15181 cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc gttttcttca ggcatgatcc
    15241 agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag tgacgaatac
    15301 ccgtacccgc actcataatc tggaattcac ccgccggaac ctgctcttta ttgcccatgc
    15361 tgtcctgatg ttcaacagta ccttccagca cgtaggtcaa aatttccata tctttatgcg
    15421 ggtgagtgcc aaagccctgc cctgcttcaa tcacgtcgtc gttaatcacg cgcagcgcgg
    15481 agaagcccat aaagttcgga tcgtaatagt tggcaaaaga gaaagtatgc caggagtcca
    15541 gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc atttgtattc
    15601 gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga tgatagaggg
    15661 tgaaaattga cccctctgtt caaaaaaatt gaactattcg aggggccatc aaacttacct
    15721 cgcgagagtc actgcggagg gagaagcttg ctcaaaaccg cgttcaagga tttccagatt
    15781 cgtaagaact tcagattcct tgacgtaatt tggcgcaccg ttggtgatgg tttgatacaa
    15841 cgtatcataa acgcgcccat aatcgcccat ctctggcttc atctcttctc ttaccgtcac
    15901 gccctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga atcctggttc
    15961 gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt atttaataaa
    16021 cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca gatggctggt
    16081 tttgacgatg gcttttagat cgccataaaa cagctgcgct tcaaaggtgt cgtccggatt
    16141 ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac cgaacagaga
    16201 aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct gcggcagccc
    16261 cggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt cgacaatctc
    16321 tcccagcttg ccgctttcaa tcgctttttt cgctgtcagg aagcaggaat caaagcgacg
    16381 attctgatac ggcgtaacga tcagcccttt gcttttcgcc agtgcaaaca gctctttcgc
    16441 ctgcgcaatt gtcggagtga acggtttttc gaccagcaca tttttcccgg cttccagcgc
    16501 gcgcttcgcg tactcgaagt ggctgtccgc gtgggtgcag acgacaacca gcttaacatc
    16561 gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgggaat aaatgggggc
    16621 ctgttcttcc ggcttcgcat ggcggcgaaa aatatgcgcg acatgccagc tatccttgcg
    16681 gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa taaaggcgca
    16741 gttaatgacc atgatttcgt cctttttaag gtggttattt acaccttagc gcaaagcgga
    16801 cgtggttcct actggagtgc gcataacgaa cacaagcact tcccgaggat aaattgagaa
    16861 cgaaaggtca aaaaaaaagc cagcacccgg ctggctaagt aatactggaa gcaatgtgag
    16921 caatgtcgtg ctttcaggtt ctccgcgagg gtcttcctga tcgcgagaca ataataatca
    16981 ttctcattcg cacttgtcca acactttttg caaaaaaatg catttgactc gcatttgaaa
    17041 gtcaatgatg ttgaaaggga catttacccc aaagaggaca aaggaatgag tgagatagtg
    17101 atacgccacg cagaaacacg ggattacgag gccatcaggc agattcacgc ccagccggag
    17161 gtgtattgca acacactaca actacctcat ccttcagaag gctggtggcc agaaagattg
    17221 aaagatcgcc aggggtttaa acaactggtt gccagcattg atggcatcgt ggtgggtcat
    17281 cttttcattg aggcttatca aggcccacgc cgtagtcatg ttgcaacttt tggtatctgt
    17341 gttgactccc gctggcacaa ccgcggcgtc gccagcgccc tgatccgcga attgattgaa
    17401 atgtgcgaca actggttacg gatagatcgt attgagttga cggtgtttgc agataacgcc
    17461 ccggcaatcg cagtctataa aaaattcggc tttcacatag aaggcaccgg caaaaattac
    17521 gcttttcgta atggtgaata tgtcgatgca tattatatgg cgcgggtgaa ataaagaatt
    17581 tgcaatataa gtttgtcatt taagccctct tcccacgaag agggccgcta accttaatac
    17641 cccgccgtta aatcatccac cgagcgcggg tcggatgcac cgtacagctc accgtccggc
    17701 ccaaccataa tgctttgtgt actgcccatc gcctctttca gcgctacttt ctgacctttt
    17761 gcttccagca gcttgagcgt atccgggcta aatcctttct cgacgcgcag ctcgtccggc
    17821 aaccactgat ggtggaaacg cggtgcattg gtcgcttcgg cgacattcat gccataatcg
    17881 atgctgttca ctaccatttg cagcacggtg gtgataatcc ggctaccgcc tgggctacca
    17941 gtcactaacc aggttttacc gtctttcacc acaatggttg gtgacatcga cgacagcggg
    18001 cgtttattcg gcccgacggc gttggcatca ccgcccacca gcccgtaaac gttcggtacg
    18061 cccggtttgg cggagaaatc atccatctgg ttattaagca gaataccgct ctcgcccgcg
    18121 acaatgcccg taccgaaggt ggtgttcagc gtataggtca ccgccaccgc gttaccatct
    18181 ttatccacca ctgagtaatg ggtagtttga ttactctcat aaggcgcaag cttgccgggg
    18241 cgaatttcgc tggatggctt cgctttattg atatcaattt gatcggcaat agatttggca
    18301 taggctttat tggtcagcgc ctgccacggg actttgacaa aatccggatc gccaagatat
    18361 tccgagcggt cggcgtaggc gtatttctca gcttctgcca tgatttgcat cgcgtcggca
    18421 ctgccaaagc catatttctt catatcgaag ttttccagga tattgaggat ctgcacgata
    18481 tgaatcccac cggaagatgg cggtggcatc gagtaaacct gatacccgcg atattcgccg
    18541 ctaatcggtg tacgttcgac cgctttatac gccgctaaat cttctttagt gatcaagcca
    18601 ccgtttttct gcatctcctg agcgatctgc tccgctattg tgcctttgta gaattcgtcc
    18661 gggccatttt cggcaatcat ctccaggctc tttgccaggt tcgcctgcac cagcttgtcg
    18721 ccctttttca gcggcgcgcc ctctttccag aagatagcct tgctgttttc gtgattcggc
    18781 aacacttcgc taccgtaggt tttgagatcg tcagccagcg cgtcattaac gataaaacca
    18841 tcgcgtgcca gtttaaacgc cggctgcacc actttattta acggcatggt gccgtattta
    18901 tccaacgcca gcgagaaacc tgctaccgta ccgggcgtac cagaagccag atgcgaagtg
    18961 agtgattttt tgctgtccgg gttaccctga tcatcaagga acatatcgcg ggtcgctttg
    19021 gcgggagcca tttcgcggaa atcgatagcg gtggtattgc catttttcga gcggatcagc
    19081 ataaaaccac caccgcccag attccctgcc tgcggatgcg ttaccgccag cgcgtagccc
    19141 acagccacgg cggcatcaac ggcattcccg ccctccttga ggatatccac ccccacctga
    19201 gtggcagtag catctacaga cgccaccatc ccctgtttcg cgcgtaccgg atgaaagaca
    19261 tcttcctcca cgccgtaaga aacaggagac ggcgcaggtg gcgcggctac cgtactaaaa
    19321 caacttcctg tgaacagagc agcaatggcc acccggcgta aaaacgtcgg ttttatcatc
    19381 gttattctcc agagattaag gggcaacccc agctaagcct ggtatataac tctgaattaa
    19441 tcctgatgat tgcaggaaag gagtaaactt aaagaatatc ttcagaggag gatacgcgat
    19501 gaaacgactt ctgattctta cggcactcct gccgtttgtc ggctttgcac agcccattaa
    19561 tactctgaac aaccctaacc agccggggta tcagatcccc agtcagcagc ggatgcaaac
    19621 ccaaatgcaa acgcagcaaa tccagcaaaa agggatgctg aatcagcaac tgaaaacgca
    19681 aactcagttg caacaacagc atttagaaaa ccagataaac aataattctc agcgggtgtt
    19741 gcagtcacag ccgggggagc gaaatcccgc ccggcagcaa atgctgccca acaccaatgg
    19801 cgggatgtta aacagcaacc gtaatccgga tagttcgttg aatcagcagc atatgctgcc
    19861 ggaaaggaga aacggcgaca tgctgaatca gcccagcacg ccgcagcctg acattccgtt
    19921 gaaaactatt gggccgtaaa gttcggacca atcacgtcaa tcgcatcggt acagatgcaa
    19981 tccacgcccc agcgcagcaa ctctgccgcg cgctggggtt tattgacggt ataaaccaga
    20041 atccgtaatc cggcgtcttt caactgcatc actcgcgctt tatcgagcaa cttatgattg
    20101 agatgaatag agacgcagcc cagccgcgcg gtcaattctc gccagtcgtc gcgccattca
    20161 tcaagcaata aaccacgcgg cagttccggt gctgcctgtt gcgcggcttc taaagcatca
    20221 atctcaaacg atgacagcag cggcggcgtc ataccggtcc acagttcgcg tgccgccagc
    20281 gccaccgttt tgcccgttaa tggcccggtg ccagtggtgg gtttgatttc gatattcgcc
    20341 atcatcccgt gttcgcgaca gcgttccgcc acctgcgaaa gcaacggcag cggctcacct
    20401 ttaaacgctt tgctgtacca actgcccgca tccacgcgca gtaaatcctg ccagttcagt
    20461 tcacccgcga cgccccagcc gttgctggtg cgttcgagat tgtcgtcatg aagcaagaag
    20521 atctcgccat ctttcgataa cttcgcgtca aattcgatca tcttatgacc gtatcttgcc
    20581 cccacatcga ttgccgccag ggtgttttcc ggggccagtt taccgccgcc acgatgggcg
    20641 acgatgcggg gataaggcca gttactcata ctcgttgtcc tgtttcacca tcaaaaagat
    20701 gcagctgatt ttccggcaga tgcagccaca gcgtgccgcc tgccgtcggg cgctcctgat
    20761 gcgccagtcg caccaccagc ttctgttcgc cccagcgtcc gtgcgccagg ttatctgcgc
    20821 cgaggatctc cagcgtgtcc atcaccagcg gcacgccacc ttcagcctgc gaacgtagcg
    20881 taatatgttc cgggcgaatg ccgagagtca ttttacgtcc agcatactga cggtagccac
    20941 cgtttagcgg cagcgcgata ccgccgtcca gtacgaaatg cgtaccttcg ttattcacac
    21001 gcccttccag cagattcatc gccgggctgc cgataaaact cgccacaaac aggctggcgg
    21061 gcttttcgta gacttcaacc ggcgtgccaa tctgttcggc aacgccgccg ttcatcacca
    21121 ttactcgctg ggcaagcgtc atcgcctcaa cctgatcgtg agtaacgtag agtgaagtcg
    21181 ttttcaggcg acggtgcagc tgttgcagtt caagacgcat ctgcacacgc aacttggcat
    21241 cgaggttaga gagcggctca tcaaacagga acactgccgg ctcacgcaca atcgcacgcc
    21301 ccatcgccac acgctgacgc tgaccgccag atagctcacg cgggcggcgc ttaagcaggc
    21361 cgtccagctc cagaatgcgc gccgcttctt taacgcgctc ggcaatttgc tgcttgccca
    21421 tgccgcgaat tttcagcccc cacgccatgt tttcttcgac gctcatatgc ggataaagcg
    21481 cgtagttctg gaacaccatc gcaatcccgc gatctttcgg ctccatttcg gtgacacgct
    21541 tgcggtcgat ccagatatcg cccgtcgtta cccgctccag tccggccacc attcgcaata
    21601 acgtcgattt cccgcagcca gacggcccga ccatcacgat aaattcgcca tccgccacat
    21661 caagggtcag cggtttaatg acctgggttt tgccatccca gcttttggtt actgcctgta
    21721 atttcagtcc tgccatctta tttctcacta tcgaccaggc cgcgcacgaa ggcacgctgc
    21781 atcactaaaa caatcactac tggtgggatc agggttaaca acatcgccgc catcaccgag
    21841 ttccattccg tggtgccttc gcctgtggcg atcatccctt taatccctgc tacggtggtg
    21901 ccgagatcca catcggtaat aatcaacaac ggccacaaat actggttcca gccgtagata
    21961 aaggtgatca caaacagcgc cgccagattg gttttggaca gtggaaaaac gatgtcgcaa
    22021 aagaaacgca ttggtgacgc gccgtcgatc cgcgcggctt ccaccagttc atccggtagg
    22081 gtcataaaga actggcgaaa cagaaacgtg gcggtcgccg aggccatcag cggcagcgtt
    22141 aaacctgcgt agctgtcgag cattttcagg ttggcgatga cttccaccgt cgggaagata
    22201 cgcacttcaa ccggcagcat cagggtgata aaaatcatcc agaagaagag gttacgtagt
    22261 ggaaaacgaa accagacaat ggcaaaggcc gagagcatcg agacggtaat tttgccgagc
    22321 gtaatgctga acgccatcac aaagctgtta agcaacatcc gccagaacgg cgcgctattc
    22381 gtgcctaccc cgttcaccca gatgttgtgg atgttttcca gcagatgcgt gccggggatt
    22441 agcgtcatcg gcgcggcgta gacggcttgt ttatccagtg tcgccgcgac aaacgccacg
    22501 tacagcggga agaggatcac cgcaatcccg aggatcagca tggtatggct gaatattgtc
    22561 agccacggac ggttctcaat cattggtaac gcaccttgct ttcaacatag cggaactgca
    22621 ccaccgtcag cacgatgacg aggaacatca acaccaccga ctgcgcggca gacgaagcca
    22681 gatccagtcc ggtaaagcct tcgcggtaaa tcttataaat cagcgtcgtg gtggcctgaa
    22741 ccggcccgcc ggacgtggcg gcgtcgatca ccgggaaggt atcgaagaag gcgtacacca
    22801 ggttcaccac cagcaggaag aaactcaccg gagcgataag cggcagagca attttaaaga
    22861 agcggcgaat cggccctgca ccgtcgatgg ctgcggcttc gatcaacgaa cggggaatgg
    22921 attgcagcgc ggcatagaag aacaggaagt tgtagctgat ttgcttccac accgaggcaa
    22981 acaccaccag aaacatcgcc tgaccgctgt tttgcgcgtg gttccagtcg tagccgaact
    23041 cagcgagaaa atgggtgatc agcccgcgac cggggttaaa caggaagatc cacaatacgg
    23101 cggcaacggc gggagccaca gcgtaaggca gcagcattaa ggtttgatag aaacggctgc
    23161 cgcgcacgat gtactccacc agcgccgcaa agaacagcga caccagcaaa ccgctgacgg
    23221 tgacaaaggt gctgaatttt atcgtcgtcc agaaggagtc gagatagtag ctgtcatgaa
    23281 acagcgtgac gaagttatcc aggccgacaa actggctgga gaaaccaaac ggatcgacgc
    23341 tttgcagcga gtaccacaac gcttcgcccg caggccagat aaaaaagata acggtgatga
    23401 tgagctgcgg cgcgaccagc aaatagggca gccagcgcga gcggaacacc ggacgggatg
    23461 atgacatagt taaactcttc gtgttgtgtt cccctcaccc cggccctctc cccagagggg
    23521 cgagagagaa aggcggtgca tactttccca ccgagcgaag ttgcaaaccg acacggcacc
    23581 aggataccac gccgatcggt cccctctccc tttgggagag ggttagggtg agggtaagag
    23641 aattaagact tcgtagactg ctcaaagcgg cgcagtaact ggttcccacg ctcaacggcg
    23701 gtatccagcg cctgctgcgg tgtcttctta ccggtccaca cgctctccag ctcttcatcc
    23761 acaatcacgc ggatctgcgg catgttgccc agacgcagcc ctttggtgaa cggtaacggc
    23821 ggcttattca gcatctgacg cgtcgcggta tccgcccctg ggtttttctc ataaaagccc
    23881 tgctcacggg tcaggtcata cgccgctttg gtgattggca gataaccggt tttctgatgc
    23941 cactcggcag cgttttctgg cttcgcgagg aagtcgagga acttcgccac gccggtatac
    24001 gtttctttat ctttgccctg catcacccac aggctggccc cgccgataat ggcgttttgc
    24061 ggtgcatctt tcgcatcggc gtcgtaaggc atcatgccta cgccgtagtt aaatttggcg
    24121 tactcacgaa tgttggcaag cgaaccggaa gaggcggtgg tcatcgcgca atcaccgtta
    24181 tagaacttct cagtggattc atccttacgc ccgacgtagc tgaaatcgcc cttcttgttc
    24241 atctcttcga gcatagcgat gtgtttcacc tgctccggct tgttgaactc cagcaccgcg
    24301 tctgtgccgt caaagccgtt gtttttgctg gcaaacggca gaccgttcca ggcgctaaag
    24361 ttttccagtt ggatccagcc ctgccagccg ctggcgtagc cgcacttcat accggaggct
    24421 ttcagtttcg cggcatagtc cgccagatcc tgccaggttt tcggcggctg ttccgggtct
    24481 aaaccggctt tcttgaaggc gtctttgttg taatagagaa caggagtcga gctattgaac
    24541 ggctgggaga gtaagtggcc cgttttgctg tcggagtagt aaccggaaac cgtcggcaca
    24601 aactgcgact catcgaattg aatccctgcc tctttaaaca cgtcatatac cggtttaatg
    24661 gctttcgatg ccatcatggt ggcggtgcca acttcataaa cctgcaaaat agccggagcg
    24721 ttgccggtac gaaatgcggc aatccccgcg cttaaattct gttcgtagtt gcctttatag
    24781 gtcggtacaa ttttgtaatc cggattttcg gcgttaaaac gttgggccag agaatccacc
    24841 tctttaccca gttccccttc catagaatgc cagaacggaa tggtcgtcac tgcctgtgca
    24901 ttccccatta acgccagtcc gagcgccagt gctgaagctg tataatgtaa cggtttcatc
    24961 gtttatctct cttgttgtac cgaatgcgcg aattcacgcg ttttatgctc gcggggtaac
    25021 atgacatgct cgaattacag aaaaataact tttttgttac atttgtaaga tagtaaggtg
    25081 tcagaaagat gacaaggcgg tgacggtacg ggtgagggaa aatgggagat ggggcacgga
    25141 taaaaagcag aatggctaag aaccttgtca ggtaccgcgt tgacgcaagc gggtaacgat
    25201 atcttctaac gatacttttc ccgccgccac atcaaccgtc aattctacaa actctgggtt
    25261 tgcagcaagt atgatgccgt tacgtttaag gaataatagc gtgataaaca gtgcggtgcg
    25321 cttattacca tcattaaaga tatgaccgcg cgagattgcc aatagatgca tcgccgccag
    25381 caaccaaaga tcagaaacgc cttcatactc atacttattc agtacccggt acaaaagcgc
    25441 ttctgctcgc cctggatcgg acattcctgt tacacctggt gtaacgctaa gcaatttatc
    25501 gtggaaataa attatctcct ccgctgagat aaactgaatg gtcatttatc cgctaactcc
    25561 ttaatttcgc tgccatgaat agccatgatg gcgtcaaatt ctgcgtccag tttagccttc
    25621 tggtaacgtt caaactcttc tgcgctgata atcacagctg ccttatgccc acgcctcgtg
    25681 atcgttaccg gagtacctgt cgccgctgtt tccagaacta atgcaaggtt ctgccgcgct
    25741 tcgctgtagt tcatggtatg catgatatcc tccatattaa gtacaattaa attgtacaac
    25801 taacaagtac aaaaaaggag tcattcgact cctttttact caaaatatta ttacccgcct
    25861 aaatacgcac ttctcaccgc ttcgttcgcc agcagcgcat cgccagtatc ggagagcacc
    25921 acatgaccgt tttccagcac gtagccgcga tcagcgagct tcagcgcctg gttggcgttc
    25981 tgctcgacga ggaagatggt catcccctgc tcgcgcagtt gctcgatggt gtcgaaaatt
    26041 tgctggatga taatcggtgc cagtccgagc gagggttcat caagcaataa caaacgtggg
    26101 ttactcatca aagcgcgacc aatcgccagc atctgctgtt caccgccgga catggtgcct
    26161 gcccgctgaa cacggcgttc atgcagacgt ggaaacagtt catacaccca ctttatgcgc
    26221 tcctggaact ggtcgcgttc agcaaaaaag ccgcccatcg ccagattctc ttccaccgtc
    26281 atgcgtgaga agacgcgacg cccttccgga acaatcgcca ccgcttcgcg catgattttc
    26341 gccgtctgcc agtcggtaat gtctttatca tcaaacacaa ttcgcccgct ggtggcgcgt
    26401 gggtcgccgc ataacgtgcc gagcaaggtg gttttccccg ccccgttcgc gccaatcagc
    26461 gtgacaattt cgccctgatt gatatgcagg ctcacctcat gcagcgcctg gattttgccg
    26521 tagtgggcgc taactttgtc aaaggacaac atgacttttt ccatcttatg cctcacctaa
    26581 ataggcacgg atcacgtccg ggttattacg gatctgctcc ggcgtaccgt ttgccagcgg
    26641 cgtcccctga ttgaccacgt aaattctgtc tgaaatcccc atcaccagct tcatatcgtg
    26701 ttcaatcaac aagatagtgg tgttgtgatg gttacgcagt tcggcaatca gctcatccag
    26761 ctctttcgtc tctttcgggt taagacctgc tgccggttcg tcgagcatta aaatctccgg
    26821 ctgcgttacc atgcagcggg cgatctccag acggcgctgg tcaccgtagg ccaggttact
    26881 cgcctgacgg ttagcgtgtt cgagcaaacc aatgcgctca agccaggttg cggcacggtc
    26941 gagcgcttcg ctctgggcgc ggcggaaaga aggcgttttc aacaggccgg agaacagccc
    27001 ggttttcagt tgctgatgct gcgccaccag caagttttca atcaccgtca tttcacgaaa
    27061 cagacgcaca tgctggaagg tgcgcaccac gcccatgcgg gcgatttgct ggccgggcaa
    27121 accttccaga tgctgatcgc gcagtaaaat agtgccaccg gtgggtttgt agaaaccggt
    27181 caggcagtta aaaaccgtgg tttttccggc accgttaggg ccgattaacg agacgatctc
    27241 ctgcgggtac agttcaagat tgacgttatt caccgccagc aggccgccga agcgcatcat
    27301 caggccgtta acagataata atggctgact catgcctgct ctcctttcgc tgcgccgttt
    27361 ttcagcttga gttgcgggcg cgtcatgggc agcaagccct gcggacgcca gatcatcatc
    27421 agcaccatca aaccaccgag cattaacatg ctgtattcgt tgaaatcacg catcaactct
    27481 cgcgacacca ccagcaaaat tgccgccaga atcaccgcaa attgcgaacc cataccaccg
    27541 agcaccacta tcgccagcac aaacgccgat tcggcaaagg tgaaggattc cgggctgaca
    27601 aagccctggc gcgcagcaaa cagcgttccg gcaaaaccgg caaacgcggc gctgatggta
    27661 aaggcggtca gcttgatacg acgcgggctt aagcccagcg aacgacaagc gatttcatct
    27721 tcacgcaacg cttcccacgc acgccccagc ggcatccgca gcaggcggtt aatgacaaac
    27781 aggcttaaca ccaccagcag caacgccacc aggtagagga agatgacgcg atcggaggga
    27841 tcgtatttca ggccaaagaa attactgaac gtgtcccagc cgccttcacg ggcggtacgg
    27901 ctgaactcga gtccgaagag cgtcggtttc ggaatctgac tgataccgtt cggtccgccg
    27961 gtaatttcgg tattattgag cagcaatatg cgcacaattt cgccgaaacc gagggtaacg
    28021 atcgctagat agtcaccgcg cagacgcaac accgggaagc cgagcaggaa gcccgccgcc
    28081 gccgccatca acccggcaat cggcagacag gtccagaagc ccagaccgta atagtgattg
    28141 agcagcgcaa aagtgtaagc gccaatggcg taaaagccgc cgtatcccag caccagcaga
    28201 ccagaaagac ctaccaccac gttcagcccg agaccgagaa taatgtagat catggtcagg
    28261 gtggcgatat ccaccgtccc acgtgaaacc ataaacggcc acgccaccgc aagcaccagc
    28321 agcgccacga ggaacagttt ctgcttcacc gtggaaccat caatcgcggg cagaataaac
    28381 ttcggcccgg aaacgctttt tagccctttc tggaaagccg gtcgcaaaag ctggaagaaa
    28441 aagacaaccg ccgtgccgat aaacacccac tgccaacgga tgtctgaagc cgtgtcgacc
    28501 accagtttgg tgccatccag ctccagttgc acgcccataa agacgcccgc caggacaaag
    28561 aacatcacag cagagagcag cgccattgca atatgcatcg gtttcatact ttctctacct
    28621 ccgggcgacc cagaataccg gtcggcatca ccagcagcac cagaatcagc agggcgaatg
    28681 agaccacatc tttatattcc gtactcagat aggcagaaga gagcgcctcc gcaatcccca
    28741 ggatcaggcc gccaatcatc gcccccggaa tgctgccaat cccaccgagc accgcagcag
    28801 taaaggcttt catcccggcc ataaagccga tgtaggggtt aattacgccg tagaactgac
    28861 cgagcagcac gcccgccacc gccgccatcg ccgcgccaat cacaaaggtc agcgcaatca
    28921 cccggtcggt gttaatgcca agcagactcg ccattttcag atcttccgcg caggcacgac
    28981 aggcgcgccc catgcgggaa tagcgaatga aaatcgtcag cgccagcatg gcgaggaagg
    29041 tgacaatcca gatcaccgcc tgcatggtgg taatagaggc agagaagttt tcgctatggc
    29101 ccaccaccca ctgaccgtta aacaggctgg gcagcgccac gtcgcgtgaa ccttcggtca
    29161 ggctgacgta gttttgcagg aagatggaca tgccgattgc ggaaataagc gcaatcaggc
    29221 gttttgagtt gcgcaccggg cgataagcca cccgttcgat actccagccg taggcgctgg
    29281 caatgacgat tgcgccgacg aatcccgccg ccaccagcag ccagctggta tcaatgccca
    29341 tcatcatcag cgcggcgatg atcataaagg agacgtagct accaatcata taaacctcgc
    29401 cgtgggcgaa gttgatcatg ccgataatgc cgtaaaccat ggtgtagccg atggctatca
    29461 gcgcgtaggt actgcccagc gtgacgccgt taaacatctg ctgcaagaaa tacaaaaact
    29521 gctcagacat aaggtaacct ttctaaaccc gcccgcattt tacgggcggt gggatgatca
    29581 cttggctgcc gtggatgaac cgtcggcgtg ccactggaag acaccaaaat caaatccctt
    29641 aagatcgcct ttttcatccc agttcagcgg cccaatcacg gtatttgcac cgttagcttt
    29701 taaatctttc accagcgcca gcggctcatc gctgccggta cgctcaaggg cagttgccag
    29761 agattgcacc gccgcgtagg tgatccagac atatggcccg gatggatctt tcttgtctgc
    29821 tttcagcgca tcaacgatgc ctttgtttgc cgggtcctgg tcatagcgtt ttggcatggt
    29881 gaccaacatg ccttcggcag catcgccagc tatgttcgac agcgacgcat tacccacacc
    29941 ttccggcccc ataaattggg ttatcaggcc gacagaacgg gcctggcgca gcatctgccc
    30001 catttccggg tagtaaccgc cgtagtaaac gaagtcgata ttttcttttt tcaggcgggc
    30061 gatcagcgcg gagaaatctt tctcacccgc ggtaataccg tcgaagaaga caacgttggc
    30121 gttagccgct ttcagcccgt cctgcaccga acgtgccagc ccttcgccat actgttgttt
    30181 gtcgtgaatg atggcgatgc gctggggctt caccgtctca agaatgtatt ttgccgccgt
    30241 tggcccctgg gaagagtcca gcccggcagt acgcatgatg tattgataac cgcgttgggt
    30301 cagctccgga ttggtcgctc ccggcgaaat catcagaata ccttcgtctt cgtagatatc
    30361 tgatgcaggt tgggtagagg aggaacacag gtgcccaata acgtatttaa tgccgtcgtt
    30421 aacgattttg ttggcgaccg caacggcttg tttcgggtcg caggcgtcgt catattccac
    30481 gccaaccagc ttatcgcctt taattccccc tttggcatta atgtctttaa tcgcctgacg
    30541 cgcgccgtta aattccatat cgccccactg ggcaatcggg ccggacatcg cgccgacaac
    30601 ggcgacttta atatcgtcag ccatagcggt gtgtgaaatt gccagtgcaa tcatccctgc
    30661 gatgatagtt ttcgcattcc gtttcatagt caaaaatccc cattcgtgat gttgtgttgc
    30721 tttgttctta tgtgttaaca aatcagacta ttcttttttt atactgcact gtttttgcct
    30781 gtctgattaa gggtttagca cagtattttg tgatgatagc gattaaaatc catatttttc
    30841 agtcgattaa gaacaga
//
LOCUS       DAPHCV010000056        30829 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.056, whole genome shotgun sequence.
ACCESSION   DAPHCV010000056 DAPHCV010000000
VERSION     DAPHCV010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.056"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..430
                     /locus_tag="RH033_002499"
     CDS             <1..430
                     /locus_tag="RH033_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="catalyzes the formation of (1 R,6
                     R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     in menaquinone biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HDV2991674.1"
                     /translation="GHPGLQNAEQRTERQRSDRQWAQRFRTEPLTAVFADWYQQPVFA
                     SLNDEQRRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSK
                     FRALAAELAAECHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            445..1302
                     /gene="menB"
                     /locus_tag="RH033_002500"
     CDS             445..1302
                     /gene="menB"
                     /locus_tag="RH033_002500"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HDV2991675.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            1302..2264
                     /gene="menC"
                     /locus_tag="RH033_002501"
     CDS             1302..2264
                     /gene="menC"
                     /locus_tag="RH033_002501"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HDV2991676.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSGLLAWVNNWLAGDSELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRDRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSLLPLVDVDALERLL"
     gene            2261..3616
                     /gene="menE"
                     /locus_tag="RH033_002502"
     CDS             2261..3616
                     /gene="menE"
                     /locus_tag="RH033_002502"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HDV2991677.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASSF
                     AMQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNDEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLDEWVKDKLARFQQPVRWLNLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            3726..3992
                     /gene="pmrD"
                     /locus_tag="RH033_002503"
     CDS             3726..3992
                     /gene="pmrD"
                     /locus_tag="RH033_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HDV2991678.1"
                     /translation="MEWLVKKSCCNKQDNRHVIMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKAAGKTQ"
     gene            complement(3986..4372)
                     /gene="arnF"
                     /locus_tag="RH033_002504"
     CDS             complement(3986..4372)
                     /gene="arnF"
                     /locus_tag="RH033_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HDV2991679.1"
                     /translation="MGLMWGLFSVIIASAAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(4372..4707)
                     /gene="arnE"
                     /locus_tag="RH033_002505"
     CDS             complement(4372..4707)
                     /gene="arnE"
                     /locus_tag="RH033_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HDV2991680.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFAAVNKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSLRHWCGVAFIIG
                     GIVILGSTV"
     gene            complement(4704..6356)
                     /gene="arnT"
                     /locus_tag="RH033_002506"
     CDS             complement(4704..6356)
                     /gene="arnT"
                     /locus_tag="RH033_002506"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HDV2991681.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLAAVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFAMDDAQHRAPLWYYLPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMSTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADVIDRQERLVLIQYRPK"
     gene            complement(6356..7246)
                     /locus_tag="RH033_002507"
     CDS             complement(6356..7246)
                     /locus_tag="RH033_002507"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HDV2991682.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQAHSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSGMLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            complement(7243..9225)
                     /gene="arnA"
                     /locus_tag="RH033_002508"
     CDS             complement(7243..9225)
                     /gene="arnA"
                     /locus_tag="RH033_002508"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HDV2991683.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPTIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACAARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCNDKYFDEDHSN
                     LTVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            complement(9225..10193)
                     /gene="arnC"
                     /locus_tag="RH033_002509"
     CDS             complement(9225..10193)
                     /gene="arnC"
                     /locus_tag="RH033_002509"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HDV2991684.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTAACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAEGSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            complement(10197..11336)
                     /gene="arnB"
                     /locus_tag="RH033_002510"
     CDS             complement(10197..11336)
                     /gene="arnB"
                     /locus_tag="RH033_002510"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HDV2991685.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLNLPAWSHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            11644..12246
                     /locus_tag="RH033_002511"
     CDS             11644..12246
                     /locus_tag="RH033_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDV2991686.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQRKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(12285..12710)
                     /gene="nudI"
                     /locus_tag="RH033_002512"
     CDS             complement(12285..12710)
                     /gene="nudI"
                     /locus_tag="RH033_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HDV2991687.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            12989..13531
                     /locus_tag="RH033_002513"
     CDS             12989..13531
                     /locus_tag="RH033_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2991688.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            13631..14833
                     /locus_tag="RH033_002514"
     CDS             13631..14833
                     /locus_tag="RH033_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HDV2991689.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLVLQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            15053..15547
                     /locus_tag="RH033_002515"
                     /pseudo
     CDS             15053..15547
                     /locus_tag="RH033_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
     gene            15478..16662
                     /locus_tag="RH033_002516"
     CDS             15478..16662
                     /locus_tag="RH033_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2991690.1"
                     /translation="MPACLATCSCTAKYYRRAGLGAGIFFVVYAFLGVPANLLMRKLG
                     ARTWIGTTTLLWGFLSAAMAWADTEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRN
                     RASIMGLFYMGAPLALTLGSPLSGALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFF
                     WLDDTPEQARFLSKEEKTLLINQLASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVA
                     VYGLIFFLPTQVAALLGTKVGFTASVVTAIPWGAALFGTWLIPRYSDKTGERRNVAAL
                     TLLAAGIGIGLSGLLSPVLAIVALCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFI
                     NLFGAVGGFIAPILRVKAETLFSSDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVA
                     HH"
     gene            16679..17482
                     /gene="yfaU"
                     /locus_tag="RH033_002517"
     CDS             16679..17482
                     /gene="yfaU"
                     /locus_tag="RH033_002517"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HDV2991691.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASHPVIRPVEGSKPLIKQVLDIGAQTLLIPMVDTA
                     DQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRVKGSY"
     gene            complement(17523..17708)
                     /locus_tag="RH033_002518"
                     /pseudo
     CDS             complement(17523..17708)
                     /locus_tag="RH033_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(17709..18668)
                     /locus_tag="RH033_002519"
     CDS             complement(17709..18668)
                     /locus_tag="RH033_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HDV2991692.1"
                     /translation="MTESTTSSPHDAVFKTFMFSPDTARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLEGWQEGKLEGLQ
                     KGKVEGMHEQAIKIALRMLEQGFEREIVLATTQLTDADIPNGH"
     gene            complement(18862..20052)
                     /gene="glpC"
                     /locus_tag="RH033_002520"
     CDS             complement(18862..20052)
                     /gene="glpC"
                     /locus_tag="RH033_002520"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HDV2991693.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(20049..21308)
                     /gene="glpB"
                     /locus_tag="RH033_002521"
     CDS             complement(20049..21308)
                     /gene="glpB"
                     /locus_tag="RH033_002521"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HDV2991694.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYTLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLKVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWSLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERDGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDEALRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(21298..22926)
                     /gene="glpA"
                     /locus_tag="RH033_002522"
     CDS             complement(21298..22926)
                     /gene="glpA"
                     /locus_tag="RH033_002522"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HDV2991695.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGINAEAIEPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGEIQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSTQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            23199..24557
                     /gene="glpT"
                     /locus_tag="RH033_002523"
     CDS             23199..24557
                     /gene="glpT"
                     /locus_tag="RH033_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HDV2991696.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            24562..25638
                     /gene="glpQ"
                     /locus_tag="RH033_002524"
     CDS             24562..25638
                     /gene="glpQ"
                     /locus_tag="RH033_002524"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDV2991697.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQIQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            25841..26491
                     /gene="inaA"
                     /locus_tag="RH033_002525"
     CDS             25841..26491
                     /gene="inaA"
                     /locus_tag="RH033_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HDV2991698.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(26545..26799)
                     /gene="yfaE"
                     /locus_tag="RH033_002526"
     CDS             complement(26545..26799)
                     /gene="yfaE"
                     /locus_tag="RH033_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HDV2991699.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(26799..27929)
                     /gene="nrdB"
                     /locus_tag="RH033_002527"
     CDS             complement(26799..27929)
                     /gene="nrdB"
                     /locus_tag="RH033_002527"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HDV2991700.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            complement(28075..30360)
                     /gene="nrdA"
                     /locus_tag="RH033_002528"
     CDS             complement(28075..30360)
                     /gene="nrdA"
                     /locus_tag="RH033_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HDV2991701.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         7258 a         7691 c         8282 g         7598 t
ORIGIN      
        1 cgggcatcct gggttgcaaa atgctgaaca acgcacggaa cgtcagcgtt ccgatcgcca
       61 atgggcgcag cgttttcgca cagaaccgtt aacggcggta tttgctgact ggtatcaaca
      121 gcctgttttt gcctcactca atgacgaaca acgccgggag ctggttgcgc tgcgcagcaa
      181 caataatggc gcaacacttg ccgccatgct ggaggcgact tctctcgccg tccagcccga
      241 tttacgtgct aaccttagcg cccgcacatt tgcgttttat tatttatgtg gtgaacgtga
      301 cagcaaattc cgcgccctgg cggcggaact ggctgccgaa tgccatgtca ttcctcgcgc
      361 cggacataac gcgcaccggg aaaatcccgc tggcgtaatc gcaagtctgg cgcagatctt
      421 gcgtttctga ctaaaggaca caatatgatt tatcctgatg aagcaatgct ttacgcaccg
      481 gttgaatggc acgactgctc cgaaggtttt gaggacattc gttatgaaaa atccaccgac
      541 ggtatcgcaa aaatcaccat taatcgtccg caggtgcgca atgccttccg tcctctgacg
      601 gtaaaagaga tgatccaggc actggcagat gcgcgttatg acgacaacat cggcgtgatc
      661 attctgactg gcgcaggcga taaagcgttc tgctccggcg gtgaccagaa agtgcgtggt
      721 gattacggcg gctataagga tgattccggc gtacatcacc tgaacgtgct ggacttccag
      781 cgtcagatcc gtacctgccc gaaaccggtt gtcgcgatgg tggctggcta ctccattggt
      841 ggcggtcacg ttctgcacat gatgtgcgac ctgactatcg cggcagataa tgccatcttc
      901 ggtcagactg gcccgaaagt cggttccttc gacggcggct ggggcgcttc ctacatggcg
      961 cgcatcgtcg ggcagaaaaa agcgcgtgaa atctggttcc tgtgccgtca gtacgacgcg
     1021 aaacaggcgc tggatatggg ccttgtaaat accgtggtgc cgctggcgga tctggaaaaa
     1081 gaaaccgtgc gctggtgccg tgaaatgctg caaaacagcc cgatggcgct gcgctgcctg
     1141 aaagctgcac tgaacgccga ctgtgacggt caggcgggtc tgcaggagct ggcgggcaac
     1201 gccaccatgc tgttctacat gacggaagaa ggtcaggaag gtcgcaacgc tttcaaccag
     1261 aaacgtcagc ctgatttcag caaatttaaa cggaatccgt aatgcgtagc gcgcaggtat
     1321 accgctggca gatccccatg gacgcggggg tggttctgcg cgacaggcgg ttaaaaaccc
     1381 gcgacgggct gtatgtttgc ctgcgtgagg gcgagcgcga agggtggggg gagatctccc
     1441 cactaccggg cttcagccag gaaacctggg aagaggcaca aagcgggctg cttgcctggg
     1501 taaataactg gctggcaggt gatagcgagc taccgcaaat gccttccgtc gcctttggcg
     1561 taagctgtgc attggcggag ctggcagaca cgctcccgca ggcggccaat tatcgtgcgg
     1621 caccactgtg taatggcgat ccggacgatc tgatcctgaa acttgccgat atgccgggcg
     1681 agaaagtggc gaaggtcaaa gtgggattgt acgaagccgt gcgcgacggc atggtggtca
     1741 atctgttgct ggaggcaatt ccggatctgc atttgcgtct tgacgcgaac cgcgcctgga
     1801 cgccgctgaa aggccagcag tttgccaaat acgttaaccc ggattatcgc gaccgcatcg
     1861 cgtttctcga agagccgtgc aaaacccgcg atgattcccg tgcgtttgcc cgtgaaaccg
     1921 gcattgccat tgcctgggat gaaagcctgc gcgagccgga ttttgccttt gtggctgaag
     1981 agggcgtgcg cgcggtagtt atcaaaccca cgctcacggg cagcctggag aaagtacgcg
     2041 agcaagtgca ggcggcgcat gcgctggggc tgacggcggt gatcagttct tccattgagt
     2101 cgagcttagg tctaacgcaa ctggcgcgga ttgctgcctg gttaacgcca gacaccattc
     2161 cgggactgga cacgctggat ctgatgcagg cgcagcaggt tcgtcgctgg ccggggagcc
     2221 tgctgccgtt agtggacgtt gatgcactgg agcggctgtt atgatcttct ctgactggcc
     2281 gtggcgtcac tggcggcaag tgcggggaga agccatcgcc ttacgtctta atgacgagca
     2341 actcaactgg cgcgagcttt gtgctcgcgt cgatgaatta gcctccagct ttgcgatgca
     2401 gggtgtggtt gagggcagcg gcgtgatgtt gcgggcgtgg aatacgccgc aaacgctgct
     2461 ggcctggctg gcgttactgc aatgcggggc gcgggtgctg cccgtgaatc cacagctgcc
     2521 gcaaccgtta cttgaggaat tgctccccaa tcttacgctg caatttgcgc tggtgccgga
     2581 tggggaaaac acgtttccgg cattaacgtc gctgcacatt cagcgggttg aaggcgcaca
     2641 tgccgctacg tggcagccga cgcgtctgtg ctcaatgacg ttgacctcgg gttctaccgg
     2701 gctgccgaaa gccgctgtcc atacttatca ggcccatctt gccagtgccg aaggggtgtt
     2761 atcgttgatt ccgtttggcg atcacgatga ctggttgctc tctttaccgc tgtttcacgt
     2821 ctccggtcag ggaattatgt ggcgctggtt atacgctggt gcgcggatga cggtgcgtga
     2881 taaacagcca ttggagcaaa tgctggcagg ctgtactcac gcttcactgg tgccaacaca
     2941 actctggcgt ttgctggtta accgcagttc cgtttccctg aaagcggtgt tacttggcgg
     3001 cgcggctatc ccggtcgagt tgacggaaca ggcgcgcgag caggggattc gttgcttttg
     3061 cggctatggt ctgaccgagt ttgcctccac ggtgtgtgcg aaagaagccg acggtctggc
     3121 agacgttggt tcgccgctgc cgggtcggga agtgaaaatc gttaatgatg aagtgtggct
     3181 gcgggctgcc agtatggcag aaggttactg gcgtaacggg caactggttt cactggttaa
     3241 tgacgaaggc tggtacgcta cgcgcgatcg cggtgagatg cataatggca agctgaccat
     3301 tgtcggacgt ttagacaatc tattcttcag tggcggagag ggtattcagc cggaagaagt
     3361 cgagcgcgta attgctgcac atcctgcggt tttgcaggtg tttatcgtcc ccgttgccga
     3421 caaggagttt ggtcatcgac cggtggcggt ggtggagtat gaccagcaaa ccgttgatct
     3481 tgacgaatgg gtgaaagaca agctggctcg ttttcaacaa ccggtgcgct ggctgaatct
     3541 gccgccggag ctgaaaaacg gcggtattaa aatttcacgt caggcgctaa aagagtgggt
     3601 gcaacgccag caataattaa ccctccgttt cacttctcat ttctgccatt ccattgccgt
     3661 tgataaagag tttatctgtt aatctgtaaa ttgatgtgaa aactcttagc aaacaggata
     3721 atgcaatgga atggctggtc aaaaaatcgt gctgcaataa acaagataac agacatgtaa
     3781 ttatgctctg cgatgctggc ggcgcgataa aaatgatcgc cgaagtgaag agtgacttcg
     3841 ccgtgaaagt gggggattta ctctcgcctt tgcagaatgc gctttactgt attaatcgtg
     3901 aaaagctgca cacggtgaaa gtcctttctg caagctgtta tagccccgat gagtgggaac
     3961 ggcagtgtaa agcggcaggg aaaactcagt aacgttgttt tgttgtgggc aggaaaatca
     4021 gcatcaaccc gctcataata caggctactc ccagtagtgc tttcagcgaa aaagtccctt
     4081 cccagcctgg aagtaccatt gaagcaatcc ataccagcac ataactcata ctgagcaagg
     4141 cataggcttt gcttagcgca agtttatgta gtgttttata ccaacaaaaa acggaaagca
     4201 gataacccag cagcccgagt aacagtatcc gggcatccag gccaaaagct aacagcgccg
     4261 cgataaaatc ccataagtgc gtcattggcg gcagatgact cgccgcaaaa cccaggctta
     4321 attgcgcagc ggaggcgata atgacgctga ataatcccca catcaggccc attacaccgt
     4381 actcccgagg atcacaatgc cgccaataat gaacgccacc ccacaccagt gacgcagcga
     4441 taccggctcg tgccacagtt ttaccgcagc cagcgtcacc cagacaaaat tcagacttaa
     4501 catcgggtag gcaataccta ccggtacgtt ctgcaagacc agcagccaga gcaccatggc
     4561 aaggccaaga caagccagcg ccagtcccag ccacagcacg atatgtttgc gccgtttgtt
     4621 taccgccgca aagcaggttg cctgtttctg acacaactgc ccggcaacgc taagcaagct
     4681 ggcaaagact aatgttagcc agatcatttg ggccgatact gaattagcac cagacgctcc
     4741 tgacgatcga tgacatctgc gggcggaatg gcgagactat tgatatcttc atcgcggtca
     4801 accgaaagca cgagtgtaat aatcccctcc tgacgatgtt gattaagcca gtttgcgaac
     4861 tcatcaccgc tgacaaatct cccttttgca tccggataat taaggccata tttcaactca
     4921 cctgtctggc gatacatgat gatgtcatcg cgttgcaggc tccatgccag acctgcggca
     4981 acaccgacgc tatcagtaag aatatagcgg cttggctgaa gtgattcttg tgtcatctcg
     5041 acaaaaaatt gcggatgttt tccttccatg actctgtcag gaattgaaaa tcctaccagc
     5101 aacgccagcc ccagcgggca aagcgcggca aaaggccagg tcttttcgac gtttgttaag
     5161 gtgtaccagc cgaaaaatgc ccatagcgaa aaaatagacc aggcacaaaa gactttatag
     5221 ctctcgaagg tttgccacac cggcgtgctc ataggtcccc agggggagac cacaaatgtg
     5281 gcaataatgc cagtgacacc aaaagcgatg ttaatccagc cattaatccg cagcgccaga
     5341 ggattatttt ttgctgccag caaagcgtaa tgcgccatca gcattgccag aggtgcaaag
     5401 caggaaagaa tataggtggg caatttgcct ttagcgacgg agaaaaacag cagcggcatt
     5461 atcgtccagc tcaataaata gacggttgcg gaatgcttgc ggtttttcca gcctgtgtac
     5521 agtgctccgg ggagtaatcc cagccacggc aggctaccgg caatgatgac cggcaagtag
     5581 taccagagcg gagctctatg ttgggcatca tccattgcaa agcgttgaat atgctcaacc
     5641 cagaaaaaat agtgccagaa gtcaggctcc cgctgcgcta tcgccagtcc ccaggggaga
     5701 accgtcagta cgcaactgat gaccgccagc cagccgtaaa taaagagatc tttccagcgt
     5761 ttttgcgttg caacccatgg aagcacgctt aataccggca cggcaagggc gagaaaacct
     5821 ttggtcatca cccccatgcc gcaagtgatt cccagcagta aaaatcccgc gcttttgcct
     5881 ttccacgtct gtgcctgcat tgccagccag aagctgcaca ttcccgccac cagccagaag
     5941 gcaataaacg gatcgagcac ggcataagtg ccaatggcat agacaataaa caatgagaga
     6001 taaatcactg cggcgagtag agccagacgt ttatcgcgcc ataagcgcag agtaaaccag
     6061 gtcaccagcg cggcagttaa cagggtcgca aagataacgc ctgcccgcac accaaagtta
     6121 ttcgcgccaa atagccattg cccaatgctg ttaatccagt atccggcaat gggtttttcg
     6181 aaataacgta gccctaacag atggggcaca atccagtcgc cggatgccag catttctcga
     6241 ctgatttccg cataacgcgt ttcatcgggt tgccatagca gacgcgtgct gatcggtaac
     6301 aggtaatagc aggcaataaa cgcgaagagg ccgataaggt aacgtaccga tttcatcagc
     6361 gactacccgc aatttgttgg caacccagcc agccttcacg tccggcaata tttccgcgaa
     6421 caacttgtcc gagcggcaac atccctgata acagttcgct taaagggcaa aatgtcacgc
     6481 cttcctgggc tgcccgttta aggagatcca caaaattatg ctgataagcg cagccttcaa
     6541 cttctgcatg aatggtataa accggcgtgc ctttatctcg caggatgcga ttgagtaacc
     6601 aaccgttaaa atcttctgct ttcacatccc gaccaatcac ttcatcccag gtgggtaagg
     6661 tcaccggaat ttgcgcagta ccagggtttc ctgattcgag cagtggacga aacggcatcg
     6721 ccccacgaca atcgctgttg tagcgcaaat ggaacgcttc ttttgcttcg atcacctgct
     6781 ggtcggcacg ccagccagcg gctgcggagc aggttaccgg ttggccgata atttcttcca
     6841 gggtgcgaag gccgcgtgcg atatcgtcga tcattgtttg ccgatcccaa ttaccgctat
     6901 gggcttgcca ggcatggtga tcccaggcgt gcaggccgac ttcgtgatgt ttagccgctt
     6961 cacgaatgat atcggcattg gcatgaccaa tctctttacc cggccaggcc gtacctgcca
     7021 gtaaaatatc ccagccataa agcgatgccg cgtttgagcg cagcatcttc cacaaaaact
     7081 gtggcttcac cagtcgccag agatggcggc ccatattgtc cgggccgacg ctgaaaaaaa
     7141 tgctggcctg aatattatgc ttactcaaga tttccagcag acgcggcacg ccttcacggg
     7201 tgccacgaaa ggtatcgaca tcaatgcgta agcctacttt ggtcatgatg gtttatccgt
     7261 aagatcaacg gtgcgcagga agaaatccag cgtttcgtcg atggtttcct gcatatcaat
     7321 tttcggttcc cagtccaggc agcggcgggc attgcggatg ctcggtttac gatgctctac
     7381 gtcctgatat cctttgccat agtagctgct actttcgaca acacgaaagc ctgcaaacgg
     7441 cgggaagtga tggcgcagcg gatgtttttc gaagctcgcc agcagcatct cgcccagttc
     7501 ctcaatgctc gcttcgttct caggattgcc aatgttgata atttcgccgt cgcagcgatt
     7561 tcccgcattt tcgataatgc ggtacaacgc ctcgatacca tcccggatat cagtaaagca
     7621 gcgtttttgt tttccgccat caattagctt aattggtgaa ccttctacca gattgagaat
     7681 gagttgcgta atagcgcggg agctgccaat tcgcgctgca ttaaggttat ccagtcgtgg
     7741 tcccatccag ttaaacggcc ggaagagggt gaactgtaaa ccctcttttt cgccataggc
     7801 ccagatcacc cgatcaagta attgttttga caccgaataa atccagcgtg gtttattcac
     7861 cgggccgacg gttaaattag aatggtcctc atcgaagtat ttatcgttac acatcccata
     7921 aacttctgaa gtcgacggga agatgattcg cttacggtac ttcacgcagt agcggataat
     7981 gcgcagattc tcttcaaaat cgagttcaaa tacgcgcagc gggttgcggg tatattcaat
     8041 cggcgtggct atcgccacca gcggcaagac gacatcacat tttttgacgt gatactcaat
     8101 ccattcggaa tgaatactga tatcgccttc gacaaagtga aaatgcggat gattcagaaa
     8161 acggcttatc gcatcgctgc caatatccag accgtaaact tcataatgat cttcgcgcag
     8221 caggcgttct gtcagatggt tgccaataaa gccattcacc ccgaggatga gtacccgggt
     8281 acggcgtcgg gcggcacagg caggctggct attcaagcgt gaaccttgca ccaggcccag
     8341 cgtctgcgct aattgcgagc cctgcatagt aatgccgtcg cccgcctgtc cggtgacgat
     8401 ttccagcgcg ccatcgccac aggcaatcag cagtggcgct acagaaatca cgctccccgg
     8461 ctgtgctttg ctggcatgag gatgaacacg cgacgaccag acggtgaatt tctgattgcc
     8521 aacatagctg aaggcacccg gccacggatc ggcaacggca cgtaccatgt tgtgcagtac
     8581 ggatgccggc ttatgccatt cgaggaaact gtcatccggc gttctgcgac caaaacaggt
     8641 agcttcgttt tcgcgctggg cgatttccag aatattaccg tgtttaatgg tgggtaatgt
     8701 ctgctccagt agctggcgcg cggcatgaca taacttatga tgcaacgtaa tggcgatatc
     8761 gtctggcgca atggcaatgc gcagttgcgc cacaatggcc ccggcatcgg cacgtttcac
     8821 cattcggtgc aatgtaacgc cagtttccgt ttcaccgttg accagcaccc agttcagcgg
     8881 cgcgcgacca cgatattttg gtaacagcga accatgcaga ttaaatgcac ctgcgggagc
     8941 gagctgcaaa atctcgtcgc aaataagatg gcgataataa aaagagaaaa tcacctctgg
     9001 tgagagttgg gcaatacgtt ccacccacag cggatgatta acgttatctg gcgcataaac
     9061 cggaatgcct ctttccgccg ccagacgagc taccgaacca taaaaggctt tttcaccggg
     9121 attatcggta tgagtaaaaa tagcgctaat ttcgtaaccg gcagccagca gggcttcaat
     9181 accgaggcat cccatatcgt ggtaggcaaa aacgacggtt ttcattattc attttccttg
     9241 ctggatggac ggataacttg ctgaacaaaa tagcgggggc gggcgcggac atcggtgtag
     9301 atcctgccga tatattcacc gagtaatccc atgccgataa actgagcgcc aataaaagta
     9361 aacagcacgg caaatagcat aaagacgcct tctgccgccc attgtggtcc gaaggtcagg
     9421 cgtaaaatca ccagcagcac cgcaatgcta aaaccgccaa tcgcaataat gctgccgagc
     9481 agactcagca tacgtagcgg cgttgtggta aggcaggtca ccaggtcgta catcaaatta
     9541 atcaggcgca taaaactgta tttggattca ccaaactcac gctcggcatg gtgcactgga
     9601 atttcaatgg cacggcgggc gaagatattc gccagaatcg ggataaaggt actgcgttca
     9661 tggcagtgca acatcgcatc gacaatatga cggcgatagg cgcgcagcat acaaccgtag
     9721 tcacccatcg ctttgccggt ggtgcgctga ataagccggt taatcatctt cgaagcggtt
     9781 ttacgaaacc agctgtcctg gcggttctgg cgtacagtcc ctaccacgtc gtaaccttca
     9841 tcggcttttg ccaccagacg ggggatttcc tctggcggat tctggagatc ggcatcgagg
     9901 gtaataatta agtcgccggt gacgtgactg aatcccgcca taatcgctga atgttgcccg
     9961 taattgcggt taagcaaaat agacacaata tggctgccct ccgcttgtga ggcttcgacc
    10021 agcatatgcg cggaattatc gctactgccg tcatcaatca gcaggatttc atactctttc
    10081 cccagtgatt cacaggctgc ggtggtgcgc ctgattaatt ccggtaagct ttcctgctcg
    10141 ttataaacgg gaataaccac cgagactttc ttaacagggt ggatttcaaa catggcttat
    10201 tgtcctgcga gttgctgaag ggctgtgatg acgcggtcgg catcggcggt ggtcatatcc
    10261 gggaacagcg gcaacgaaca gatgcgttcg ctattccatt cggtattcgg taacgacagc
    10321 gtgggaaaac gctcgcgata atatttttgt gtgtgagcgg cgcggaaatg taacccggta
    10381 ccaatgcctc tttcttttaa cgcttccatc aacgcatcgc gactgatacc acaacgttgt
    10441 tcatcgacac gaataataaa cagatgccag gcgtgaacgt gtgaccaggc gggaaggttt
    10501 aatggctgaa aggggagagc tgccagtgct tgctgatatt gctgggcaat ttcgcgccga
    10561 cgggtgttga ggtgctctaa tttgactaac tgtgtcaggg caatcgcggc gttaatatcg
    10621 gtcagattgt acttatagcc cggcgttaag acttcagctt gcggtgcacg gccccaggtt
    10681 tgtctgtcat aggcatcgac acccagaccg tgaaatttta gcatccgtag ctggcgggca
    10741 aggttttcat tatctgtaac aatcaggcca ccttccgcac aggtaatatt cttgatggcg
    10801 tgaaacgaaa aaatagcggt accttttgcg ccaatatgtc gccctttgta atacgtacct
    10861 acggcatggg cagcatcttc gataactgcg atgccgtaac gttcgccaat ggcgcgaatg
    10921 gcgtcaatat ctgctggcgc accggcataa tgtaccggaa tgatggcttt agtgcgtggc
    10981 gtaatggctg actcgatcgc ttcaggcgtg accatcagcg tatcgcggtc gacatccacc
    11041 attaccggcg ttgcacccaa caaggagatc atattgaggg ttgaaaccca ggtcagggaa
    11101 ggcgtaatca cttcatcgcc cttgccaatt tccaacgcca ttagcgtgat atgcattccg
    11161 gcggtggctg aactgaccgc gatggcatgc tgatttcccg tcaactggca aaaggcttgt
    11221 tcaagcgcct gattcttcgg accggttgtg atccaaccgg attcgagaac ctctttaact
    11281 gctgcgagtt cctccacgcc cattgctggt cgcgaaaaag gcaaaaattc tgacattgct
    11341 tttccttccg ccattgaata cctgtccact tatatttgct ataaagagtg ttgtgtatat
    11401 tttgccattt ggagcgaaat tctaaggata gaatattaac ttaaccttaa gaaactaata
    11461 ttagacgtaa atattgaaat ttttatattt tttcttattt aggctttgca tttggcaaaa
    11521 ttttgaggca ttttgccggc atcgtaggat ttttaatatt acaccaactg ctaattatcg
    11581 ccagaaatgt cgctcaattt cacggtaatt gtctggttgc gcttgtctat aggtggagtt
    11641 tacgtgttag ctttttgccg ctcttcgttg aagtcaaaaa aatatttcat cattttactg
    11701 gcgctcgctg caattgccgg actgggtact catgccgcct ggagtagcaa tggtttgcca
    11761 cgtatcgaca ataaaacgct ggccagactg gcacagcagc atccggttgt cgttttgttt
    11821 cgccatgctg aacgttgcga tcgttcaacc aatcaatgct tgtcagataa aacaggtatt
    11881 acggtcaaag gtacccagga tgcccgtgaa ctgggcaacg cttttagtgc tgatatccct
    11941 gatttcgatc tctattccag taataccgtc cggaccattc agtcggcgac ctggttttca
    12001 gcgggtaaaa agctgacggt agataaacga cttcttcaat gcggtaatga gatttatagt
    12061 gcaattaaag acttacaacg caaagcgcct gataaaaata tcgttatttt tacccataat
    12121 cattgcctga catatattgc taaagataag cgtgatgcga catttaaacc tgattatctg
    12181 gatggtttag tcatgcatgt ggaaaaaggc aaagtttatc tggatgggga attcgttaac
    12241 cactaaattt tcaatctgac agccagtgat ggctgtcatc attgttaaag aagacctttc
    12301 aaacgtaacg tttttcgggt ggcaacattc aaatcataat gcaccagatc ttcaggtttt
    12361 acccacgcgt agtcctgaaa ctcttcgttt attttcactt ctcggttggc agaaacgcag
    12421 tcaaaaatca ggtaaatcat ataaatctct tctttgcgac catctgcata cgtcttggtg
    12481 cgaatatcat cgctgaaggt ccacggcgtg atttctgtca aaagcagctg ttctcccagc
    12541 tcttcgcgaa tttcgcggcg tagtgcctct tcaattcgtt cgccaggctc cacgccgcca
    12601 cccgaaagcg cccattgacc agggaaaacg ccgcgatcgt cggccatttt acacagcaaa
    12661 taagcaccat cattttgaat caaagggcat acaatagtcc gttgtcgcac aggggtattc
    12721 cttataaata aaaagaaacc atgagaatac ggtaactcag aaatatgtga aagtgacagt
    12781 aatctctttt gcgatgataa ctgcacttat tattattttt gtgtgtatcg gtaaagtatg
    12841 tttatttttt cggcgtttaa ttatttaaga agtatttagt tacaataagt gactagatcc
    12901 cttttttcgc tcattgaatc ctctgtgcca ggcagtacaa tcccgcagct ttgggaattt
    12961 tgatttattg cttgtgggag aggggaaaat gaaaaaaatt gcgctagcag gtctggccgg
    13021 aatgctgttg gtttctgcat cggtcaatgc aatgagcatc agcggccagg cgggtaaaga
    13081 atacaccaat attggtgtcg gttttgggac tgaatcgacg ggcctggcgt taagcggtaa
    13141 ctggacacat aacgacgacg acggtgacgt cgctggcgtg gggctggggt tgaatctgcc
    13201 tctcgggccg ttaatggcga ccgttggcgg aaaaggcgtt tacaccaacc cgaattacgg
    13261 cgatgaaggt tatgccgcag cggtaggagg tggtttgcag tggaaaattg gcaacagctt
    13321 ccgtttgttt ggcgagtatt actactctcc ggattcgctc tccagcggta ttcaaagtta
    13381 tgaggaagcg aatgctggcg cgcgttacac cattatgcgt ccagtcagta ttgaggcggg
    13441 ttatcgctac ctgaatctgt cgggtaaaga cggtaaccgc gacaacgcag tggctgacgg
    13501 cccgtatgtg ggggttaacg ccagtttctg atcccttccg gcgcggcatt ctgtcgcgcc
    13561 attcagtttc ttactacgcc ttatctccct ttgcgttata gtgattcgat aacaaaagca
    13621 ggagataacc atgttaaaag tggaaatgct atccaccggg gatgaagtgt tacacgggca
    13681 aatcgttgac actaacgctg cctggctggc cgattttttc tttcatcagg ggttgccatt
    13741 atctcgccgc aatacggtgg gggataacct tgatgactta gtcaccattc ttcgcgaacg
    13801 tagtcagcac gccgatgtgc tgatcgttaa cggcgggctg ggaccgacca gcgatgattt
    13861 aagcgcactc gccgctgcga cagcaaaagg tgaaggcctg gtgttgcatg aagcctggct
    13921 caaagagatg gaacgctatt tccacgaacg tggacgagta atggcaccga gcaaccgtaa
    13981 acaagcggag ctgcctgcca gtgctgaatt tatcaataac ccggtaggca ccgcctgtgg
    14041 ttttgccgtg cagcttaatc gttgcctgat gttctttacc cccggcgtac cgtcagaatt
    14101 taaggtgatg gtcgagcacg aaatcctgcc gcgcctgcgc gagcgttttt ctttaccaca
    14161 gccgccggtt tgtctgcgtt taactacctt tggtcgttcg gaaagcgatc tggcacaaag
    14221 cctggacact ctacaactgc cgccgggcgt aacaatgggt tatcgctcct caatgcctat
    14281 catcgaactg aaactcaccg gaccggcaag cgagcaacag gcgatggaaa aactgtggct
    14341 ggacgttaaa cgtgttgccg gacagagcgt gattttcgaa ggcaccgaag gactgcccgc
    14401 gcagatcagt cgcgaattgc aaaaccgcca gttcagcctg acgttgagcg agcaattcac
    14461 cggcggttta ttggttttgc aactttctcg cgcaggtgct ccattgctgg cgtgtgaagt
    14521 ggttccttca caggaagaaa ccctggcgca aactgcgcac tggattacag aacggcgggc
    14581 caaccatttt gccgggctgg cactggctgt ttcgggtttc gagaacgagc atctcaactt
    14641 tgcgctagcc acgccagacg gcactttcgc tctgcgtgtg cgtttcagca ctacgcgcta
    14701 cagcctggct atccgtcagg aagtgtgcgc aatgatggca ctgaatatgc tgcgccgttg
    14761 gttaaacggc caggacatcg ccagtgagca tggctggatt gaggttgttg agtccatgac
    14821 tttatctgtc tgattccgcc actctgtgcc ggtttactgc accggcacat tccccctccg
    14881 gcaaaatttg tccaacctgt tgtactcaat aagaaaaaat ccgtgcgcta attcacaaat
    14941 ttgtgcgtaa tttctccagt ccgctggacg aggagcggga tttgatgcgc ttcacaattt
    15001 tactccccct gagtaacgag actgattcca gtatctaaga ctggagtcca gtatgttgga
    15061 atcaagcaaa gttcccgccc tgacacgggc tattgatatt ctcaatttga ttgcccgcat
    15121 tggtccctgt agtgctgcga cgatcattga cacattgggg atccctaaaa gcacggccta
    15181 tttgctgctt aatgaactca ggcgtcagcg ttttcttagc cttgatcacc aggaaaattt
    15241 ttgtttgtgg accaggctgg tggagctttc cggtcatgcg ttaagcaaga tggatctccg
    15301 ggaactggct cgaccgcgtt tgacgcaact gatggatacc actgggctgt tatgtcacct
    15361 ggggattatt gataacggca gcgcttatta cattttgaaa gtggagtcat cagccactat
    15421 cagcgtgcgt tcccatgaag gaaaaagcct ttcgctttat cgctccggaa taggtaaatg
    15481 cctgcttgcc tggcaacctg cagctgtaca gcaaagtatt atcgaagggc tggtctggga
    15541 gcaggcattt tctttgtggt atacgcgttt ctgggtgttc cggcgaatct tttgatgcgc
    15601 aaactggggg ccagaacctg gattggtacg acaacactgc tgtggggatt tctttcggct
    15661 gccatggcat gggccgatac tgaagcgaaa tttctgatcg ttcgcactct gcttggtgct
    15721 gcggaggccg gttttttccc tggtatgatt tatctcactt cgcaatggtt tccgcagcgt
    15781 aatcgcgcca gcattatggg gctgttctat atgggcgcac cgttggcgtt aacactggga
    15841 tcaccgcttt ccggcgcgct gctggagatg catggattta tggggcatcc cggctggttc
    15901 tggatgtttg tgattgaagg attgctggca gtcggcgctg gggtattcac attcttttgg
    15961 cttgatgaca cgccggagca ggcacgtttt ctgagtaaag aagaaaaaac gttgcttatc
    16021 aaccaactgg caagtgaaga acaacagaaa gtgacttctc gactgagcga tgcgctgcgt
    16081 aatgggcgag tctggcaact ggcgattatc tacctgacca ttcaggtggc ggtttacgga
    16141 ttaattttct tcctgccgac gcaggttgct gcattgttgg ggacaaaagt gggctttacg
    16201 gcgtcggtgg tcaccgccat tccgtggggc gcggccttgt ttgggacctg gcttattccg
    16261 cgctattccg ataaaaccgg cgagcggcgt aatgtcgcgg cgctgacatt actggcggca
    16321 ggcattggta ttggtctctc cgggctgctt tctccagtac tggcgatcgt tgcgctgtgt
    16381 gttgcagcca tcgggtttat tgccgtacag ccagtgttct ggacgatgcc gacacagctt
    16441 ctttccggta cggcgctggc tgcggggatt ggttttataa acctgtttgg tgcagtgggc
    16501 gggtttattg ccccgatcct gcgcgtgaaa gcagaaacgt tattttccag cgatgcggcg
    16561 ggattactga cgctggcagc ggtggcggtc atcggttcgc tgattatttt cactctgcgt
    16621 gtaaatcgca ctgttgcgca gaccgacgtg gcacatcatt aaatagttaa ggaacacgat
    16681 gaacgcatta ttaaccaatc cctttaaaga acgtttacgc aagggcgaag tgcaaattgg
    16741 tctgtggtta agctcaacga ctgcctatat ggcagaaatt gccgccactt ccggttatga
    16801 ctggttgctg attgacgggg agcatgcgcc aaacaccatt caggatcttt atcaccagct
    16861 acaggcggta gcgccctatg ccagccaccc cgtgatccgt ccggtggaag gcagtaagcc
    16921 gctgattaaa caagtcctgg atattggcgc gcaaactcta ctgatcccaa tggtcgatac
    16981 tgccgatcag gcacgtcagg tggtgtctgc cacgcgctat cctccctacg gtgagcgtgg
    17041 tgttggggcc agtgtggcac gggctgcgcg ctggggacgc attgagaatt acatggcgca
    17101 agtcaacgat tcgctttgtc tgttggtgca ggtggaaagt aaaacggcac tggataacct
    17161 ggacgaaatc ctcgatgttg aagggattga tggcgtgttt attggacctg cggatctttc
    17221 tgcgtcgttg ggctacccgg ataacgccgg gcacccggaa gtgcagcgaa ttattgaaac
    17281 cagcattcgg cggatccgtg ctgcgggtaa agcggctggt tttctggctg tggctcctga
    17341 tatggcgcag caatgcctgg cgtggggagc gaactttgtc gctgttggcg ttgacacgat
    17401 gctctacagc gatgccctgg atcaacgact ggcgatgttt aaatcaggca aaaatgggcc
    17461 acgcgtaaaa ggtagttatt gatatcaaag gcccatgggg atcggctgtg ggcctgtgtt
    17521 aattagtggt tattcgctgc cagatcggct tcgcttagct gagtggccgc gagcacctga
    17581 tcacgatcaa tgccctgctc cagcatgcgc aaagcaattt taatggcttg ttcatgcatg
    17641 ccttcctgcc agccaatttg atgtccttcc tgccgtagcc gttcagcaat agtcattaat
    17701 ctctcctttt aatgaccgtt cggaatatca gcatcagtga gttgggttgt cgccagcaca
    17761 atctcacgtt caaagccctg ttccagcatg cgcaaggcaa ttttaatggc ttgttcatgc
    17821 ataccttcta ctttgccttt ctgcaaaccc tccaatttcc cctcctgcca gccttctaat
    17881 ttaccttcct gccagccaat ttgatgtcct tcctgccgta gccgttcagc aatagtcatt
    17941 aatctctcct tttgtagtgg tgaacgttcg gcaatctcct gaataaagcg ggtgaaacgg
    18001 gaggtatcgc cgcattgcag cagataatta aacagcgttt gtagctggct gtcattagtg
    18061 aagcctctaa ccaaaagcgt ggtgatcctg tcgaccatac cgattaaatc gcggtcgcga
    18121 atatgctttt gaatcagttc cagcagagct atacgccgat gttgcatgat ctcatcgtca
    18181 ggtacgatgg tgatatccac caacggaaaa gcttcggtgt acaactgccg ggcaagttgc
    18241 ggatcgtcaa actcatccag ccagttgagt gagtacgggt agggcgaggt ttcgccatga
    18301 taaaacagca atggcaccac cagcggaact ctgtcatagc ctttatccag gtgacgttgc
    18361 atggcggcag tggcatagcg cattagtcga aaagccatat tcttttctgc agaactttga
    18421 tgttcaatca cgcagtagat atagccgtca ccgtcgctgg tttccaccga ccacaaaaca
    18481 tccgagtagt aagcgcgtaa acttttttca ataaagctgg tgggttccag gcgtaaggtt
    18541 tgcaggttgc aaagcttgcg cagcggctct ggcagatgta tttcgagaaa atctcgcgcc
    18601 gtatcgggcg agaacataaa ggttttaaat accgcatcat gcggggagga ggttgttgat
    18661 tcggtcatgg ttgtccgtca cctgatattt atgataacgc gacattacgg ttatcccagg
    18721 cctttgacat ctgccagttt cagcctttgc gagacgtttt cagaggaatt ttcactgcgt
    18781 tgcaacgtat acattgagcg cggaatcgtg gacgcaaaaa tgcccggtaa caacatgctt
    18841 accgggcatc agatgggagt tttaagccag cgcctgggcc agtagcgtaa tcggatgttc
    18901 gcagcgaaga cttgtggaca tctcaatctg ccatttgcag gtttcgcagt cggtaaccac
    18961 cagatctgcg ccgctttctt ctatctgacg gaacagtggt gcgccgatgg cttgtgaggt
    19021 ggggtagttc tcttttttga aaccgtaagt acctgcaata ccgcagcact gggaatccag
    19081 caccgttaac tcaagccccg ggattttacg caacagctcc agggtgtaga gcgtccagcc
    19141 cattttttcc atatggcacg gagtgtgata aaccactttt agcggcagcg gtttcagcgg
    19201 caaggttttg ccttcgtcca gcttgcgcca cagccagcgg gtcgccagtt cgatgtgatc
    19261 gcgcaagcct ttgttgtcga cattcagcac ttccgggtac tcgtcgcgca gagcaaacgt
    19321 acaggtcgag gaggtggcaa tcaccggaat gccttttact cccactgctt cgcggatcga
    19381 ctctacattt gtgattgcct gtttacgtgc tttatcggta aagccgttgg cgatcagcgg
    19441 tacgccgcag catttttctt tgctgagcag ttgtacaccg gtacccattg cgttgagcac
    19501 tttaattaaa tctttgccta actgcggatg gttgtagtta acgaagcagc cgtgaaagaa
    19561 agccacctgg tctttatatt gcgcttgttg ggctgccacg ctgcgatacc agcgacggaa
    19621 cgtgccgaag gagtatttcg gtagcgtgcg gcgatgatcg atttttaacg ccgcatcaag
    19681 cagctgtcgc accggtttca gcgaggtagc agtgttaacg attggtgcga atggcgtgga
    19741 aacgctaccc atcaggtcgg tatgactcaa cacaaaattg cgcagtgacg ggcgtgtggt
    19801 gtcatatttc gcccgcgcgc gctggatgat atcgccaatc ttcacatcgg acgggcaggc
    19861 gacttcgcaa cgtttgcagt tgatgcaata tttcagtgcc tcgtcataca gtgcgccatc
    19921 cttcaaacgc agacgctcgc catccggccc ggcttgtttt ggccccggat atcccggatt
    19981 cacccggctc accgggcagg cggtggtgca gacggtgcac ttaatgcagt tttcgaagct
    20041 ggtgtcgttc attgttggcc tcctgcgcgt tgggcaatct gttgagcggc atgtaaagca
    20101 ctgacggcac aaacaccgcc gccgcatccc tgggcgatgg gatcaaatcc gcccagcacc
    20161 gaaccgatgg caaacaggtt ctcaatggtt tgccctgcct gcgacgggcg tagtgcctca
    20221 tcagtggtta caccgaactg ctgccacggt tgcggcgcaa aaaaatcgcc cttataccat
    20281 tcaccccgcg tggcggtttg tagcacatca aggccgagaa tcggctcgcg aatgccgtca
    20341 cgttctgcca ccagtccgcc actaaagaaa ctgccgctcg caagcaccgc aaatcgtggg
    20401 cgcagcggga tatcggcgtg attgcgggtc cagatttcat ttactacgcc atttttacag
    20461 gtcacttttt tcacttcatc gcctggcatc cacacgccac cctggcgcac aaactggcgc
    20521 tgtaactggt tttgcagacg aatgcccagc acggaaggcg gcagcgttgg caaaagcatc
    20581 aatgaacaag gtagtttttc attcaaccaa cgccagagtt tgtcatcggc caggccgaag
    20641 caggcgggca tcaggatcat ttcgcaggta ttggcgacag gaataagcgc atcaagtaac
    20701 agcgaccagt tttcttcatt atcaaggaaa cgggcgatat tcaccgcgcg aaactcggtg
    20761 gcgttgttgc gcagcacatc cagttccggc agttcaattt cagcggtttc aactttaaga
    20821 tccaattcgc gcaatgaagc cgctgcaaga tgcgcctgaa aatccatcag accgctaatt
    20881 cctactacac atattttctt cgcgggcagc ggccagacgg ggacttctgg cgaacttagc
    20941 caggttgagc gcagagtgcc gagtggcgta atccgctggt gggccagttc tacgctgccc
    21001 tgtaattgcg caccgctttc agcgattaat gcctgcgcct ggcaagcgag atcgagtacg
    21061 cgttgtggcc ctagaagagt gtaaggatgg gcaggtgcct gctgacgcaa agattccagt
    21121 ccactgtgaa tgtctgtcac cggttggcca tctggcagat ggctcagcaa atccagcgat
    21181 ccagatgaga aatggagcgc gctttgacca cgagtgacaa tggcacagcg caggccgtgt
    21241 ttttgcagtt gcaggccaca gagtaatccg gcgaggccgc cgcccataat gacagtatca
    21301 aagcgcatct ttctgctcct tctccagacc acacagtccc tgataaaccc agcgggtaaa
    21361 ttcgctttcg cgcagtgcgt ctccccatgc tatgggttgc acgcctttcc agcgttcgtt
    21421 taggaaggtg gaaagttgct cgatagattg cgtggacgtc gtgacgttaa aacgttgcag
    21481 cagtcctgca gcgcgacagg cgcagagttc gccctggcat gtgcccattc ccacacgggt
    21541 acgacggcgt aaatccagca ggctgttaac gtttaaattt tctactgcgt actgcacttc
    21601 acccgcagtt accgcttcgc actcacatac caggctgcgg tgcaggcggc cttcgctcag
    21661 ccaggctggt gtgcgatcgc cgtgccggta aactgcggaa ccgcgcagcg gagcgggcag
    21721 ggagatcact ttgcgcagcg tgacttctgc cggttcctgt gagccaggaa gtgccagatc
    21781 ggcggttgta caggggcgcg tgttgcccag cttgcgacat accgcgtcgg tagcccattc
    21841 cgccatcagg cggtaggtca tcagtttgcc accggtgatg gtgataaacc cgtcgagacc
    21901 atcgcgttca gcatgatcga gcagcacgat gccacggctg acgttacgtc cgctcggatc
    21961 gtcatcgctg gcaaccagcg ggcgtacgcc agagtaggcc cgcaaaatgc gcgttttcgc
    22021 catcacgggg gccagttttt ccccttcacg cagcagaata tcaacttctt ctgccgtcac
    22081 tcgattatcg tcaatctcgt tgtaatcaat acgtaaagag gtggtgccaa tcagcgaaat
    22141 ggtatcgcca ggcaccagaa tatcggcgtc ggaaggtttg cggcagcggt tgatcacatg
    22201 ctgattaatg cggtgatcca tgatcagcag cgatcctttc gccgggaaca tgcgaatgcg
    22261 cagatcggca tattcggcaa tgtgttgccc ccagatcccg gcggcattaa ctacgacagg
    22321 cgcatgaagt gcctgaattt cgccggtgag atggttacgt acacgaacac cgcacaccgt
    22381 cgcgccttca cgaatcagcc ccgtgacttc atgagcggta aggataacgg caccgtgttc
    22441 tttggcatcc agcatgtttg ctgctgtcag acgaaatgga tcaacggtgc catccggaac
    22501 tttcaccgcg ccaatcagtg ccgggttaac ggcaggttcg ataatgcgcg cttgctgcgg
    22561 ctcaatggct tctgcgttga tccctgcttc ttcgcaggcg cggataaaag tggcctggaa
    22621 ggagaggtca tcttccggca gggtgataaa caggccgttg gttggttcta cgcagtgacg
    22681 tgcaatgcgt ttcagaatct ggttttcact aatacattcg cgggccgatt ccgcatcggt
    22741 taccgcatag cgcgcaccgc tgtgcagcag gccgtggtta cgcccggtgg caccggttgc
    22801 gatgtcgtgg cgctcaacca aaatcacgcg cagcccgcgc agggcacagt cgcgggcaat
    22861 cccggctccc gttgcgccac cgccaatgat aatcacgtca cttgattgcg agtcgcgagt
    22921 tttcattgtt atccctctga agttcgtttt ttaccattta gccatagtaa aaacatgaat
    22981 tgtttgattt cgcgcatatt cgctcataat tcgaaagtga aacgtgattt catgcgtcat
    23041 tctgaacatt tcaaccatcg tatttaataa tgtgtgcggt aattcacatt taatttatga
    23101 atgttttctt aacatcgcgg caactcaaga aacggcaggt tctcccactg aatcaggctg
    23161 ttaatcataa ataagaccac gggccacgga ggctatcaat gttgagtatt tttaaaccag
    23221 cgccacacaa agcgcgctta cctgccgcgg agatcgatcc gacttatcgt cgattgcgct
    23281 ggcaaatttt cctggggata ttctttggct atgctgctta ctatttggtt cgtaagaact
    23341 ttgcgctcgc tatgccttat ctggttgagc agggattctc acgcggtgat ttaggttttg
    23401 ccctttcggg gatctcgatt gcttatggat tttcgaaatt catcatgggt tcggtatcgg
    23461 atcgctcgaa tccgcgcgtt ttcctgcccg caggtttgat tctggcggcg gcagtgatgt
    23521 tgtttatggg ctttgtgcca tgggcgacgt cgagcattgc ggtgatgttt gtactgttgt
    23581 tcctctgcgg ttggttccag gggatggggt ggccgccgtg tggtcgtact atggtgcact
    23641 ggtggtcgca gaaagaacgt ggcggtattg tgtcagtgtg gaactgtgcg cacaacgtcg
    23701 gtggtggtat tccgccgctg ctgttcctgc tggggatggc ctggttcaat gactggcacg
    23761 cggcgctcta tatgcccgct ttctgtgcca ttctggtggc actgttcgcc tttgcgatga
    23821 tgcgcgatac cccgcaatcc tgtggcctgc cgccgatcga agagtacaaa aatgattatc
    23881 cggacgacta taacgaaaaa gcggaacagg agctgaccgc gaagcagatc ttcatgcagt
    23941 acgtgctgcc gaacaaactg ctgtggtaca tcgccattgc caacgtgttc gtttatctgc
    24001 tgcgttacgg catcctcgac tggtcaccga cttatctgaa agaggttaag catttcgcgc
    24061 tggataaatc ctcctgggcc tacttccttt atgaatatgc aggtattccg ggcaccctgc
    24121 tgtgcggctg gatgtcggat aaagtctttc gtggtaaccg tggggcaacc ggcgtcttct
    24181 ttatgacact ggtgaccatc gcgactatcg tttactggat gaacccggca ggtaacccaa
    24241 ccgtcgatat gatttgtatg attgttatcg gcttcctgat ttatggccct gtgatgctga
    24301 tcggtctaca tgcgctggaa ctggcaccga aaaaagcggc aggtacggca gcgggtttta
    24361 ccgggctgtt tggttacctc ggcggttcgg tggcggcgag cgcgattgtt ggctacaccg
    24421 tggacttctt cggctgggat ggcggcttta tggtaatgat tggcggcagc attctggcgg
    24481 ttatcttgtt gattgttgtg atgattggcg aaaaacgtcg ccatgaacaa ttactgcaaa
    24541 aacgcaacgg aggctaatgg catgaaattg acgctgaaaa accttagcat ggcgatcatg
    24601 atgagcacta tagtcatggg aagcagtgca atggcggcgg acagcaacga aaaaatagtc
    24661 atcgcccatc gcggtgccag tggatatttg ccggagcata cgctgccagc aaaagcgatg
    24721 gcgtatgcgc agggagcgga ttatctggaa caggatttgg tgatgaccaa agacgaccat
    24781 ctggttgttc tgcatgacca ttatctcgat cgtgttactg atgttgccga tcgtttcccg
    24841 gatcgggcgc gcaaagacgg tcgttactac gcgatagatt tcacgctgga tgaaattaag
    24901 tcgctgaaat ttaccgaagg tttcgatatt gaaaacggta aaaaagtgca gacttatccg
    24961 gggcgtttcc caatgggtaa atctgacttc cgggtgcaca cctttgaaga agagattgaa
    25021 tttgttcagg ggttaaacca ctctaccggg aaaaatatcg gtatctatcc agaaattaaa
    25081 gcgccgtggt tccatcatca ggaagggaag gatattgcgg caaaaacgct ggaagtgctg
    25141 aagaaatatg gttacaccgg taaggacgac aaagtttatt tgcaatgttt tgatgctgat
    25201 gagctgaagc gtattaagaa tgagctggaa cctaaaatgg gtatggatct caatctggta
    25261 caactgattg cctataccga ctggaacgaa acgcagcaga tacagccgga cggaagctgg
    25321 gttaattaca actacgactg gatgtttaag ccgggggcta tgaaacaggt ggcggaatat
    25381 gctgatggta ttggtccgga ttaccatatg ttgattgagg agacatcgca gccgggtaat
    25441 atcaaactca ctggcatggt gcaagatgct cagcagaata aactggtagt gcatccttat
    25501 accgtgcggt cagataaact gcctgaatac actactgatg tgaatcagtt atatgatgct
    25561 ctgtataaca aagcgggtgt aaatgggcta tttactgatt tccctgataa ggcagtaaaa
    25621 ttccttaata aagagtaaat taactaccgg atggcactta tgtgctatcc gataccactc
    25681 ttcattaaag tctgtgggtt atccttaatt gcaattattt tgagcgagat gacttgttcc
    25741 cggcataaga tactcgtcaa tacaacacgt attattaaga ttttcctcag ctaaacatct
    25801 atagtcattg tcgttaataa aaaacgacag gcgatagggt atggcagttt cagcaaagca
    25861 cgacgaattt aatcactggt gggcaacaga gggcgactgg gttgaagagc ctaactatcg
    25921 ccgtaatggt atgagcgggg tgcaatgcgt cgagcgcaac ggcaaaaagc tgtatgtaaa
    25981 gcgcatgacg catcatctgt ttcattccgt acgttatccg ttcggccgac caacgattgt
    26041 ccgtgaggtt gcggtaatta aagaactgga acgggccggt gtcatcgtgc cgaagatcgt
    26101 ttttggtgaa gcggtgaaaa ttgagggtga atggcgcgcg ttgctggtga ctgaagatat
    26161 ggcggggttc atcagcattg ctgactggta tgcccggcat gcagtatcgc cttattctga
    26221 cgaagtacgg caagccatgt tgaaagcagt ggcgctggcg tttaagaaaa tgcatagcgt
    26281 taatcgtcag catggctgtt gttatgttcg ccatatctat gtgaaaacag aaggtaaggc
    26341 agaagctggt tttcttgatc tggagaaaag tcgtcgccgc ttgcgtcgtg ataaagcgat
    26401 taatcatgac ttccgccagc tggaaaaata tctggagccg atccctaaag cggactggga
    26461 gcaggtgaaa gcgtattact acgccatgta atcgttgtag gccagataat acgcgtaagc
    26521 gtcgtatctg gcgctagtac ccgatcacaa ctcgatttca atatcgcctt ttgcccggca
    26581 acaacagggc aaaatttccc ccggctgaat aaaggctaac ggttcggcaa tccagtccac
    26641 ctggcctgca acaaggcgcg tgcgacagga accgcagtaa ccttcgcggc actggtactc
    26701 aaccgccaca ttgtgggatt ccagcgccgc cagaagggaa gggtgttcat cctggcacag
    26761 cagttgtgtg ccagtgatgc gcagggtaac gcgagccatc agagctggaa gttactcaaa
    26821 tcgtcggtgt ccacttccga gtcaatctgc ccgaccagat aagaactgac ttccacttcc
    26881 tgcggagcaa cctgcacgtt atcagacacc agccaggtat tgatccacgg gatcgggttg
    26941 gagcgtgtct ggaacggcag atccaaaccg actgcctgca tgcggatatt ggtgatgtat
    27001 tcaacgtact ggcagagaat gtctttattc agaccaatca tcgaaccgtc acggaacaga
    27061 taatccgccc agtctttctc ttgctgtgcc gcctggacaa acaggtcata gcactcctgc
    27121 ttacattctt cggcaatttc cgccatctcc ggatcatccg cgccgctgcg cagcagattc
    27181 agcatatgct gagtaccggt caggtgcagg gcttcatcgc gggcaatcag gcgaataatt
    27241 ttggcgttac cttccatcag ttcgcgttct gcaaaggcaa aggaacaagc aaagctgacg
    27301 tagaagcgga tcgcttccag cgcgttaacg ctcatcaggc agagatacag ttttttcttc
    27361 agctcgcgca ggctaacggt tacggtttta ccgttaacgg tgtgggtacc ttcgcccagc
    27421 agatgccagt agctggtcat ttcgatcagc tcatcgtaat agctggagat cccttccgca
    27481 cgtttctgga tctgctcgtt ggtaacgata tcgtcaaaga caacagacgg atcgttaacg
    27541 atattacgga tgatatgggt ataggagcgg gaatgaatcg tttctgagaa cgcccaggtt
    27601 tctacccagg tttccagttc cgggatcgag atcagcggca gcagtgccac gttcgggctg
    27661 cgaccctgaa tggaatccag cagcgtctga tatttcaggt tgctgataaa gatgtgtttt
    27721 tcgtgttcag gcagtgcctg gtaatcgata cggtcgcggg agacgtcaac ttcttccgga
    27781 cgccagaaga aagagagctg cttttcgatc agcttttcga agatgtcata tttttgctga
    27841 tcgtagcgag ccacgttgac cggctgacca aagaacattg gttctttgag ctgatcattt
    27901 ttcgtctgtg aaaaggtggt atatgccatg aatgtgtcct gttgggagtt taatgccgga
    27961 taaggcattt tacgccgcat cctgtgcctg atgcgacgct ggcgcgtctt atcaggccta
    28021 caaaccgagc cgtatgccgg ataaggcgtt cacgccgcat ccggcatctc aatatcagat
    28081 cttacatgcg ccgctttcgc agccatcgtc ctggattgac ggtaccagat cgtcttgtgc
    28141 gtcttcagca ccgtcacggg tgttctgata atacagcgtt ttgaccccga atttgtaggc
    28201 ggtgagcagg tctttcagca gttgctgcat cggcactttt cctgacggga agcgtgacgg
    28261 atcgtagttg gtgttagcag agatcgactg atcgataaat ttctgcatga tacccaccag
    28321 ttgcaggtag ccatcgttac ccggcatttc ccacagcagt tcataggcgt cgtgcagatg
    28381 ctcgtagtcc ggcaccacct ggcgcagaat accgtctttc gacgctttga tgctgacgta
    28441 accacgcggc ggttcaatac cgttagtggc gttagagatc tgcgaagagg tctcggacgg
    28501 catcagggca gaaagcgtgg agttacgcag gccgtgcgtt ttgattgact cacgcagtgc
    28561 ttcccagtcg taatgcagcg gctcattagc gatggcatcc agatctttct tataggtatc
    28621 gatcggcagg atccctttcg cgtaagtggt ttcgttaaac cacgggcacg cgccttgctc
    28681 tttcgccagc tcattagacg ctttcagcag gtaatactga atggcttcga aggttttatg
    28741 cgtcaggttg ttggcgctgc cgtcggagta gcgtttaccg tgcttcgcca gatagtaagc
    28801 gaagttgatc acaccaatac ccagcgtacg acgccccatc gctccacgtt tagcggccgg
    28861 gatcgggtaa tcctgataat ccagcagtgc gtcgagtgcg cgaaccgcca gaattgccag
    28921 ctcttccagt tcatccaggc tgttaattgc gcccaggttg aaagcagaaa gcgtacacag
    28981 cgcgatttca ccgttctcgt cgttgacgtc gttcagtggt ttggtcggta gggcgatttc
    29041 caggcacagg ttagactggc gcactggtgc aatagccgga tcaaacgggc tgtgggtgtt
    29101 gcagtggtct acgttctgaa tatagatacg accggtagac gcacgttcct gcatcatcag
    29161 cgagaacagc tcaacggctt tcacacgctg cttgcggatg ctgtcgtctt tctcatattt
    29221 ggtgtacagg cgttcaaact cttcctgatc ggcgaagaac gcgtcgtaca gccccggtac
    29281 gtcggacggg ctgaacaggg tgatatcttc acctttcagc agacgggtat acatcagttt
    29341 gttgatttgt accccgtagt ccatatgacg cacgcggttg ccttcaaccc cacggttgtt
    29401 tttcaacacc agcaggcttt ccacttccag atgccacatc gggtagaaca gtgttgccgc
    29461 accgccgcgc acaccgccct gagagcagga tttcaccgct gtctggaaat gtttgtaaaa
    29521 cggaatacag ccggtgtgga atgcttcgcc accgcgaatc gggctaccca acgcacgaat
    29581 acgcccggcg ttgatgccga tcccggcacg ctgggaaacg tatttaacaa tcgcgctgga
    29641 ggtggcgttg atggaatcca ggctgtcgcc gcactcgatc agtacgcagg agctgaactg
    29701 acgagtcggg gtacgcacgc cggacatgat tggcgtcggc agcgaaattt taaatgtgga
    29761 aaccgcgtcg taaaaacgct tcacgtattg caggcgcgtt tcacgcgggt agttcgagaa
    29821 caggcacgca gcaaccagaa tatagaggaa ctgggcgctc tcatagattt cgccggtcac
    29881 gcggttctgt accagatatt tgccttccag ctgcttaacg gcagcataag agaaggtcat
    29941 atcacggtcg tgatcgataa acgtgtccat ctgcttgaac tcttcttccg tgtagtcttc
    30001 cagcagatga ttatcgtatt tgcccatctc gaccattttc accacgtggt cgtacagcgc
    30061 aggcggctca aactggccgt aggctttttt acgcaggtgg aagatcgcca ggcgcgcggc
    30121 gagatactga taatccggcg catcacggga gatcaggtct gcggcagcct tgataatggt
    30181 ttcgtggatg tcagaggtct tgataccgtc ataaaactga atgtgggagc gcagttcgac
    30241 ctgggaaatc gaaacgttat gcagtccttc tgccgcccaa tccagaacgc gatggatttt
    30301 gtcgagattg atgcgctctg tgctaccgtc gcgctttgtc accagcagat tctgattcat
    30361 gtatgtcgta cctgtttttg gaattctttc cgcaatagtt tcatgcagac tacctgtagt
    30421 gagggaagct gcttagaaca ctatatatag ggggtatgtt tgggattcac tgcaagatag
    30481 tgtgaaaatg gccctcttgc aagtgcataa ctttgtggat aactcaggaa ggaaaaagtg
    30541 gctttcgcgc accttaggtc agacaaggtg tccgggaaag tcaatgggaa gagaaaaatt
    30601 tgttaaaaat aactgttcgg cgatttcttg aacggaagaa taggttagat aaattgatat
    30661 agatggttta ttaatctttt ggctaatgat tgcaaaaatt gatctaagca cgagtcatca
    30721 cataatctga tgtatcagtt atggtcaata tataagcgaa tgtaagcgat tgttgatgtc
    30781 gaaatggttg ttttctcttc ggttgttgtt aaatgcgaag ccattttgt
//
LOCUS       DAPHCV010000057        30656 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.057, whole genome shotgun sequence.
ACCESSION   DAPHCV010000057 DAPHCV010000000
VERSION     DAPHCV010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.057"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..578
                     /gene="tuf"
                     /locus_tag="RH033_002529"
     CDS             <1..578
                     /gene="tuf"
                     /locus_tag="RH033_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDV2991702.1"
                     /translation="ERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVG
                     IKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKF
                     ESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTL
                     IHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            808..1191
                     /gene="secE"
                     /locus_tag="RH033_002530"
     CDS             808..1191
                     /gene="secE"
                     /locus_tag="RH033_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDV2991703.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1193..1738
                     /gene="nusG"
                     /locus_tag="RH033_002531"
     CDS             1193..1738
                     /gene="nusG"
                     /locus_tag="RH033_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDV2991704.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1897..2325
                     /gene="rplK"
                     /locus_tag="RH033_002532"
     CDS             1897..2325
                     /gene="rplK"
                     /locus_tag="RH033_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDV2991705.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2329..3033
                     /gene="rplA"
                     /locus_tag="RH033_002533"
     CDS             2329..3033
                     /gene="rplA"
                     /locus_tag="RH033_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDV2991706.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3446..3943
                     /gene="rplJ"
                     /locus_tag="RH033_002534"
     CDS             3446..3943
                     /gene="rplJ"
                     /locus_tag="RH033_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDV2991707.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            4010..4375
                     /gene="rplL"
                     /locus_tag="RH033_002535"
     CDS             4010..4375
                     /gene="rplL"
                     /locus_tag="RH033_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDV2991708.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4695..8723
                     /gene="rpoB"
                     /locus_tag="RH033_002536"
     CDS             4695..8723
                     /gene="rpoB"
                     /locus_tag="RH033_002536"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDV2991709.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8800..13023
                     /gene="rpoC"
                     /locus_tag="RH033_002537"
     CDS             8800..13023
                     /gene="rpoC"
                     /locus_tag="RH033_002537"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDV2991710.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAVGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13263..14396)
                     /gene="thiH"
                     /locus_tag="RH033_002538"
     CDS             complement(13263..14396)
                     /gene="thiH"
                     /locus_tag="RH033_002538"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HDV2991711.1"
                     /translation="MKTFSDRWRQLDWDNIRLRINGKTAADVERALNVSQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNVVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSISFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRP"
     gene            complement(14393..15163)
                     /gene="thiG"
                     /locus_tag="RH033_002539"
     CDS             complement(14393..15163)
                     /gene="thiG"
                     /locus_tag="RH033_002539"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HDV2991712.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15165..15365)
                     /gene="thiS"
                     /locus_tag="RH033_002540"
     CDS             complement(15165..15365)
                     /gene="thiS"
                     /locus_tag="RH033_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HDV2991713.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRD
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15349..16104)
                     /gene="thiF"
                     /locus_tag="RH033_002541"
     CDS             complement(15349..16104)
                     /gene="thiF"
                     /locus_tag="RH033_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HDV2991714.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16097..16732)
                     /gene="thiE"
                     /locus_tag="RH033_002542"
     CDS             complement(16097..16732)
                     /gene="thiE"
                     /locus_tag="RH033_002542"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDV2991715.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLSAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLPRAPAVMATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16732..18627)
                     /gene="thiC"
                     /locus_tag="RH033_002543"
     CDS             complement(16732..18627)
                     /gene="thiC"
                     /locus_tag="RH033_002543"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HDV2991716.1"
                     /translation="MSATKLTRREQRAQAQHFIDTLEGTAFPNSKRIYITGTQPGVRV
                     PMREIQLSPTLIGGSKEQPQFEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARRGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSVQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18708..18808)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18860..19336)
                     /gene="rsd"
                     /locus_tag="RH033_002544"
     CDS             complement(18860..19336)
                     /gene="rsd"
                     /locus_tag="RH033_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HDV2991717.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19431..20204
                     /gene="nudC"
                     /locus_tag="RH033_002545"
     CDS             19431..20204
                     /gene="nudC"
                     /locus_tag="RH033_002545"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HDV2991718.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20244..21308
                     /gene="hemE"
                     /locus_tag="RH033_002546"
     CDS             20244..21308
                     /gene="hemE"
                     /locus_tag="RH033_002546"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDV2991719.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21465..22136
                     /gene="nfi"
                     /locus_tag="RH033_002547"
     CDS             21465..22136
                     /gene="nfi"
                     /locus_tag="RH033_002547"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HDV2991720.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22179..22769
                     /locus_tag="RH033_002548"
     CDS             22179..22769
                     /locus_tag="RH033_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HDV2991721.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAVFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22956..23228
                     /gene="hupA"
                     /locus_tag="RH033_002549"
     CDS             22956..23228
                     /gene="hupA"
                     /locus_tag="RH033_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HDV2991722.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23241..23936
                     /locus_tag="RH033_002550"
     CDS             23241..23936
                     /locus_tag="RH033_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HDV2991723.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23938..24363)
                     /gene="zraP"
                     /locus_tag="RH033_002551"
     CDS             complement(23938..24363)
                     /gene="zraP"
                     /locus_tag="RH033_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HDV2991724.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLHQSLD
                     ELRVKRDIAMAEAGIPRGTGMGMGYGGCGGGSHMGMGHW"
     gene            24601..25977
                     /gene="zraS"
                     /locus_tag="RH033_002552"
     CDS             24601..25977
                     /gene="zraS"
                     /locus_tag="RH033_002552"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HDV2991725.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSGQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVELNTLISHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVVSQEGKGATFTLWLPVT
                     ITRKDPQG"
     gene            25974..27299
                     /gene="zraR"
                     /locus_tag="RH033_002553"
     CDS             25974..27299
                     /gene="zraR"
                     /locus_tag="RH033_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HDV2991726.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPVVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLANHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENTVERAVVLLTGEYISERELPLAIASTPIP
                     LVQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27296..28585)
                     /gene="purD"
                     /locus_tag="RH033_002554"
     CDS             complement(27296..28585)
                     /gene="purD"
                     /locus_tag="RH033_002554"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDV2991727.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHQIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     QEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYHTGDVIHGLPLEEVSDGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28597..30186)
                     /gene="purH"
                     /locus_tag="RH033_002555"
     CDS             complement(28597..30186)
                     /gene="purH"
                     /locus_tag="RH033_002555"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDV2991728.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEI
                     DANEGSLTLETRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGHSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7186 a         7981 c         8458 g         7031 t
ORIGIN      
        1 cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc tccatctccg
       61 gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt ggtgaagaag
      121 ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt gaaatgttcc
      181 gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg cgtggtatca
      241 aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc aagccgcaca
      301 ccaagttcga atctgaagtg tacattctgt ccaaagatga aggcggccgt catactccgt
      361 tcttcaaagg ctaccgtccg cagttctact tccgtactac tgacgtgact ggtaccatcg
      421 aactgccgga aggcgtagag atggtaatgc cgggcgacaa catcaaaatg gttgttaccc
      481 tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa ggcggccgta
      541 ccgttggcgc gggcgttgta gcaaaagttc tgagctaatt gccgataaca tttgacgcaa
      601 tgcgcactaa aagggcatca tttgatgccc tttttgcacg ctttcgtacc agaacctggc
      661 tcatcagtga ttttctttgt cataatcatt gctgagacag gctctgttga gggcgtataa
      721 tccgaaaagc taatacgcgt ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt
      781 tgtagaatac ttctgacagg ttggtttatg agtgcgaata ccgaagctca aggaagcggg
      841 cgcggcctgg aagcgatgaa gtgggtcgtt gtggtggcat tgctcctggt ggcgattgtc
      901 ggcaactatc tttatcgcga cattatgctg ccgctgcgtg cgctggccgt agtaattctg
      961 attgctgcag cgggtggtgt cgcgctgtta acgacaaaag gtaaagctac cgttgctttt
     1021 gcccgtgaag cgcgtaccga agtccgtaag gtcatttggc cgactcgcca ggaaacattg
     1081 cacaccacgc tgattgtggc tgcggttacc gcagtaatgt cactgatcct gtggggactg
     1141 gatggtattc tggttcgcct ggtatccttt atcactggcc tgaggttctg agatgtctga
     1201 agctcctaaa aagcgctggt acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc
     1261 aacgtcgctg cgtgagcata tcaaattaca caacatggaa gatttgtttg gtgaagtcat
     1321 ggtaccaacc gaagaagtgg ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa
     1381 attcttccct ggctacgtcc tcgttcagat ggtgatgaac gacgcgagct ggcacctggt
     1441 gcgcagcgta ccgcgtgtga tgggcttcat cggcggtact tccgatcgtc ctgcaccaat
     1501 cagcgataaa gaagtcgatg cgattatgaa ccgcctgcag caggttggtg ataagccgcg
     1561 tccgaaaacg ctgtttgaac cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga
     1621 cttcaacggt gttgttgaag aagtggatta cgagaaatct cgtctgaaag tgtctgtttc
     1681 tatcttcggt cgtgcgaccc cggtagagct ggacttcagc caggttgaaa aagcttaacc
     1741 cagcgatcaa aaaagcggcg atttaatcgt tgcacaaggc gcgagattgg aatacaattt
     1801 cgcgcctttt gtttttatgg gccttgcccg taaaacgatt ttttatatca cggggagcct
     1861 ctcagaggcg ttattaccca acttgaggaa tttataatgg ctaagaaagt acaagcctat
     1921 gtcaagctgc aggttgcagc tggtatggct aacccgagtc cgccagtagg tccggctctg
     1981 ggtcagcagg gcgtaaacat catggaattc tgcaaagcgt tcaacgcaaa aactgattcc
     2041 atcgaaaaag gtctgccgat tccggtagta atcaccgttt acgctgaccg ttctttcact
     2101 ttcgttacca agactccgcc agcagcagtt ctgctgaaaa aagcggctgg tatcaagtct
     2161 ggttccggta agccgaacaa agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa
     2221 atcgcgcaga ccaaagctgc cgacatgact ggtgccgaca ttgaagcgat gactcgctcc
     2281 atcgaaggta ctgcacgttc catgggcctg gtagtggagg actaagaaat ggctaaactg
     2341 accaagcgca tgcgtgttat ccgcgagaaa gttgatgcaa ccaaacagta cgacatcaac
     2401 gaagctatcg ctctgctgaa agaactggcg actgctaaat tcgtagaaag cgtggacgta
     2461 gctgttaacc tcggcatcga cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta
     2521 ctgccgcacg gtactggccg ttccgttcgc gtagccgtat ttacccaggg tgcaaacgct
     2581 gaagctgcta aagctgcagg tgctgaactg gtaggtatgg aagatctggc tgaccagatc
     2641 aagaaaggcg aaatgaactt tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt
     2701 ggccagctgg gccaggttct gggtccgcgc ggcctgatgc caaacccgaa agtgggtact
     2761 gtaacgccga acgttgctga agcggttaaa aacgctaaag ctggccaggt tcgttaccgt
     2821 aacgacaaaa acggcatcat ccacaccacc atcggtaaag tggactttga cgctgacaaa
     2881 ctgaaagaaa acctggaagc tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg
     2941 aaaggcgtgt acatcaagaa agttagcatc tccaccacca tgggtgcagg tgttgcagtt
     3001 gaccaggctg gcctgagcgc ttctgtaaac taatgccttt acgtgggcgg tgattttgtc
     3061 tacaatctta cccccacgta taatgcttaa tgcagacgta tatccgagat attcgggttg
     3121 tggcaaggcg gtaactgagc gagtcgccag gagcatagtt aactatgtga ctggtgcgaa
     3181 cgaaggaagc caacgccgcc acaagctgaa tagcgacgga tagaaaagat ttgttcgttg
     3241 gagcctggcc tatccaggcc tccgtcgaag accgcaggag tttcgcaaga aacttaatcc
     3301 cctgcgtaga cggtgacaga acgctaagat tattctttta tattctggct tgtttctgct
     3361 caccgtaatt aagacgctct ctccgtttgg aggagtgaag tgagttccag agattttctc
     3421 tggcaaacat ccaggagcaa agctaatggc tttaaatctt caagacaaac aagcgattgt
     3481 tgctgaagtc agcgaagtag ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg
     3541 cgtaactgta gataaaatga ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat
     3601 gcgtgttgtt cgtaacaccc tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct
     3661 gaaagacgcg tttgttggtc cgaccctgat tgcatactct atggaacacc cgggcgctgc
     3721 tgctcgtctg ttcaaagagt tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc
     3781 ctttgaaggt gagctgatcc cggcgtctca gatcgaccgc ctggcaactc tgccgaccta
     3841 cgaagaagca attgcacgcc tgatggcaac catgaaagaa gcttcggctg gcaaactggt
     3901 tcgtactctg gctgctgtac gcgatgcgaa agaagctgct taatcgcagt tatcttttta
     3961 acgcattcgc ttacgtataa acttattctg atattcagga acaatttaaa tgtctatcac
     4021 taaagatcaa atcattgaag cagttgcagc tatgtctgta atggacgttg tagaactgat
     4081 ctctgcaatg gaagaaaaat tcggtgtttc cgctgctgct gctgtagctg tagctgctgg
     4141 cccggttgaa gctgctgaag aaaaaactga attcgacgta attctgaaag ctgctggcgc
     4201 taacaaagtt gctgttatca aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc
     4261 taaagacctg gtagaatctg caccggctgc tctgaaagaa ggcgtgagca aagacgacgc
     4321 agaagcactg aaaaaagctc tggaagaagc tggcgctgaa gttgaagtta aataagccaa
     4381 cccttccggt tgcagcctga gaaatcaggc tgatggctgg tgacttttta gtcaccagcc
     4441 tttttgcgct gtaaggcgcc agtagcgttt cacactgttt gactactgct gtgcctttca
     4501 atgcttgttt ctatcgacga cttaatatac tgcgacagga cgtccgttct gtgtaaatcg
     4561 caatgaaatg gtttaagcgt gatagcaaca ggcattgcgg aaagtgttcc attttccggt
     4621 caacaaaata gtgttgcaca aactgtccgc tcaatggaca gatgggtcga cttgtcagcg
     4681 agctgaggaa ccctatggtt tactcctata ccgagaaaaa acgtattcgt aaggattttg
     4741 gtaaacgtcc acaagttctg gatgtacctt atctcctttc tatccagctt gactcgtttc
     4801 agaaatttat cgagcaagat cctgaagggc agtatggtct ggaagctgct ttccgttccg
     4861 tattcccgat tcagagctac agcggtaatt ccgagctgca atacgtcagc taccgccttg
     4921 gcgaaccggt gtttgacgtc caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc
     4981 tgcgcgttaa actgcgtctg gtgatctatg agcgcgaagc gccggaaggc accgtaaaag
     5041 acattaaaga acaagaagtc tacatgggcg aaattccgct catgacagac aacggtacct
     5101 ttgttatcaa cggtactgag cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct
     5161 tctttgactc cgacaaaggt aaaacccact cttcgggtaa agtgctgtat aacgcgcgca
     5221 tcatccctta ccgtggttcc tggctggact tcgaattcga tccgaaggac aacctgttcg
     5281 tacgtatcga ccgtcgccgt aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca
     5341 ccacagagca gatccttgac ctgttctttg aaaaagttat ctttgaaatc cgtgataaca
     5401 agctgcagat ggaactggtg ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg
     5461 aagctaacgg taaagtgtac gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc
     5521 agctggaaaa agacgacgtc aaactgatcg aagtcccggt tgagtacatc gcaggtaaag
     5581 tggttgctaa agactatatt gatgagtcta ccggcgagct gatctgcgca gcgaacatgg
     5641 agctgagcct ggatctgctg gctaagctga gccagtctgg tcacaagcgt atcgaaacgc
     5701 tgttcacaaa cgacctggat cacggcccgt atatctctga aaccttacgt gtcgacccaa
     5761 ctaacgaccg tctgagcgca ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc
     5821 cgactcgtga agcagctgaa agcctgttcg agaatctgtt cttctccgaa gaccgttatg
     5881 acctgtctgc ggttggtcgt atgaagttca accgttctct gctgcgcgaa gaaatcgaag
     5941 gttccggtat cctgagcaaa gacgacatca ttgatgttat gaaaaagctc atcgatatcc
     6001 gtaacggtaa aggcgaagtc gatgatatag accacctcgg caaccgtcgt atccgttccg
     6061 ttggcgaaat ggcggaaaac cagttccgcg ttggcctggt acgtgtagag cgtgcggtga
     6121 aagagcgtct gtctctgggc gatctggata ccctgatgcc tcaggatatg atcaacgcca
     6181 agccgatttc tgcagcagtg aaagagttct tcggttccag ccagctgtct cagtttatgg
     6241 accagaacaa cccgctgtct gagattacgc acaaacgtcg tatctccgca ctcggcccag
     6301 gtggtctgac ccgtgaacgt gcaggcttcg aagttcgaga cgtacacccg actcactacg
     6361 gtcgcgtatg tccaatcgaa acccctgaag gtccgaacat cggtctgatc aactctctgt
     6421 ccgtgtacgc acagactaac gaatacggct tccttgagac tccgtatcgt aaagtgaccg
     6481 acggtgttgt aactgacgaa attcactacc tgtctgctat cgaagaaggc aactacgtta
     6541 tcgcccaggc gaactccaac ctggatgaag aaggccactt cgtagaagac ctggtaactt
     6601 gccgtagcaa aggcgaatcc agcttgttca gccgtgacca ggttgactac atggacgtat
     6661 ccacccagca ggtggtatcc gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg
     6721 ccaaccgtgc attgatgggt gcgaacatgc aacgtcaggc cgttccgact ctgcgtgctg
     6781 ataagccgct ggttggtact ggtatggaac gtgctgttgc cgttgactcc ggtgtaacgg
     6841 cggttgctaa acgtggtggt gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag
     6901 ttaacgaaga cgagatgtat ccgggtgaag caggtatcga catctacaac ctgaccaaat
     6961 acacccgttc taaccagaac acctgtatca accagatgcc gtgtgtgtct ctgggtgaac
     7021 cggttgaacg tggcgacgtg ctggcagacg gtccgtccac cgacctcggt gaactggcgc
     7081 ttggtcagaa catgcgcgta gcgttcatgc cgtggaatgg ttacaacttc gaagactcca
     7141 tcctcgtatc cgagcgtgtt gttcaggaag accgtttcac caccatccac attcaggaac
     7201 tggcgtgtgt gtcccgtgac accaagctgg ggccagaaga gatcaccgct gacatcccga
     7261 acgtgggtga agctgcgctc tccaaactgg atgaatccgg tatcgtttac attggtgcgg
     7321 aagtgaccgg tggcgacatt ctggttggta aggttacgcc gaaaggtgaa actcagctga
     7381 ccccagaaga aaaattgctg cgtgcgatct tcggtgagaa agcgtctgac gttaaagact
     7441 cttctctgcg cgtaccaaac ggtgtatccg gtacggttat cgacgttcag gtctttactc
     7501 gcgatggcgt agaaaaagac aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg
     7561 cgaagaaaga cctgtctgaa gaactgcaga tcctcgaagc gggtctgttc agccgtatcc
     7621 gtgctgtgct ggtagccggt ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc
     7681 gctggctgga gctgggcctg accgacgaag agaaacaaaa tcagctggaa cagctggctg
     7741 agcagtatga cgaactgaaa cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa
     7801 tcacccaggg cgacgatctg gcaccgggcg tgctgaagat tgttaaggta tatctggcgg
     7861 ttaaacgccg tatccagcct ggtgacaaga tggcaggtcg tcatggtaac aagggtgtaa
     7921 tttctaagat caacccgatc gaagatatgc cttacgatga aaacggtacg ccggtagaca
     7981 tcgtactgaa cccgctgggc gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc
     8041 acctgggtat ggctgcgaaa ggtatcggcg acaagatcaa cgccatgctg aaacagcagc
     8101 aagaagtcgc gaaactgcgc gaattcatcc agcgtgcgta cgatctgggc gctgacgttc
     8161 gtcagaaagt tgacctgagt accttcagcg atgaagaagt tatgcgtctg gctgaaaacc
     8221 tgcgcaaagg tatgccaatc gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta
     8281 aagagctgct gaaacttggc gacctgccga cttctggtca gatccgcctg tacgacggcc
     8341 gcactggtga acagttcgag cgtccggtaa ccgttggtta catgtacatg ctgaaactga
     8401 accacctggt cgacgacaag atgcacgcgc gttccaccgg ttcttacagc ctggttactc
     8461 agcagccgct gggtggtaag gcacagttcg gtggtcagcg tttcggggag atggaagtgt
     8521 gggcgctgga agcatacggc gcagcataca ccctgcagga aatgctcacc gttaagtctg
     8581 atgacgtgaa cggtcgtact aagatgtata aaaacatcgt ggacggcaac catcagatgg
     8641 agccgggcat gccagaatcc ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca
     8701 acatcgaact ggaagacgag taattctcgc tcaaacaggt cactgctgtc ggggtaaaac
     8761 ccggcagcgg attgtgctaa ctccgacggg agcaaatccg tgaaagattt attaaagttt
     8821 ctgaaagcgc agactaaaac cgaagagttt gatgcgatca aaattgctct ggcttcgcca
     8881 gacatgatcc gttcatggtc tttcggtgaa gttaaaaagc cggaaaccat caactaccgt
     8941 acgttcaaac cagaacgtga cggccttttc tgcgcccgta tctttgggcc ggtaaaagat
     9001 tacgagtgcc tgtgcggtaa gtacaagcgc ctgaaacacc gtggtgtcat ctgtgagaag
     9061 tgcggcgttg aagtgaccca gactaaagta cgccgtgagc gtatgggcca catcgaactg
     9121 gcttccccga ctgcgcacat ctggttcctg aaatcgctgc cgtcccgtat cggcctgctg
     9181 ctcgatatgc cgctgcgcga tatcgaacgc gtactgtact tcgaatccta tgtggttatt
     9241 gaaggcggta tgaccaacct ggaacgccag cagatcctga ctgaagagca gtatctggac
     9301 gcgctggaag agttcggtga cgaattcgac gcgaaaatgg gtgcggaagc aatccaggcc
     9361 ctgctgaaga gcatggatct ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa
     9421 accaactccg aaaccaagcg taaaaagctg accaagcgta tcaaactgct ggaagcgttc
     9481 gttcagtctg gtaacaaacc agagtggatg atcctgaccg ttctgccggt actgccgcca
     9541 gatctgcgtc cgctggttcc gctggatggt ggtcgtttcg cgacttctga cctgaacgat
     9601 ctgtatcgtc gcgtcattaa ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg
     9661 ccggacatca tcgtacgtaa cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg
     9721 gataacggtc gtcgcggtcg tgcgatcacc ggttctaaca agcgtcctct gaaatctttg
     9781 gccgacatga tcaaaggtaa acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt
     9841 gactactccg gtcgttctgt aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt
     9901 ctgccgaaga aaatggcgct ggagctgttc aaaccgttca tctacggcaa gctggaactg
     9961 cgtggtcttg ctaccaccat taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc
    10021 gtttgggata tcctggacga agttatccgc gaacacccgg tactgctgaa ccgtgcaccg
    10081 actctgcacc gtctgggtat ccaggcattt gaaccggtac tgatcgaagg taaagctatc
    10141 cagctgcacc cgctggtttg tgcggcatat aacgccgact tcgatggtga ccagatggca
    10201 gttcacgtac cgctgacgct ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc
    10261 aacaacatcc tgtccccggc gaacggcgaa ccaatcatcg ttccgtctca ggacgttgta
    10321 ctgggtctgt actacatgac ccgtgactgt gttaacgcca aaggcgaagg catggtgctg
    10381 actggcccga aagaagcaga acgtctgtat cgctctggtc tggcctctct gcatgcgcgc
    10441 gttaaagtgc gtatcaccga gtatgaaaaa gatgctaacg gtgaattagt agcgaaaacc
    10501 agcctgaaag acacgactgt tggccgtgcc attctgtgga tgattgtacc gaaaggtctg
    10561 ccttactcca tcgtcaacca ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc
    10621 tgctaccgca ttctcggtct gaaaccgacc gttatttttg cggaccagat catgtacacc
    10681 ggctttgcat atgcagcgcg ttctggtgca tctgttggta tcgatgacat ggtcatcccg
    10741 gagaagaaac acgaaatcat ctccgaggca gaagcagaag ttgctgaaat tcaggagcag
    10801 ttccagtctg gtctggtaac tgcgggcgaa cgctacaaca aagttatcga tatctgggct
    10861 gcggcgaacg atcgtgtatc caaagcgatg atggataacc tgcaaactga aaccgttatt
    10921 aaccgtgacg gtcaggaaga gaagcaggtt tccttcaaca gcatctacat gatggccgac
    10981 tccggtgcgc gtggttctgc ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg
    11041 gcgaagccgg atggctccat catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg
    11101 aacgtactcc agtacttcat ctccacccac ggtgctcgta aaggtctggc ggataccgca
    11161 ctgaaaacgg cgaactccgg ttacctgact cgtcgtctgg ttgacgtggc gcaggaccta
    11221 gtggttaccg aagacgattg tggtactcat gaaggtatca tgatgactcc ggttatcgaa
    11281 ggtggtgacg ttaaagagcc gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac
    11341 gttctgaagc cgggtactgc tgatatcctc gttccgcgca acacgctgct gcacgaacag
    11401 tggtgtgacc tgctggaaga gaactctgtc gacgcggtta aagtacgttc tgttgtatct
    11461 tgtgacaccg actttggtgt atgtgcgcac tgctacggtc gtgacctggc gcgtggccac
    11521 atcatcaaca aaggtgaagc aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt
    11581 acacagctga ccatgcgtac gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa
    11641 tccagcatcc aggtgaaaaa caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg
    11701 aactccagcg gtaaactggt tatcacttcc cgtaacaccg aactgaaact gatcgacgaa
    11761 ttcggtcgta ccaaagaaag ctacaaagta ccttacggtg cggtactggc aaaaggcgat
    11821 ggcgaacagg ttgctggcgg cgaaaccgtt gcaaactggg acccgcacac catgccggtt
    11881 atcaccgaag taagcggttt tgtacgcttt actgacatga tcgacggtca gaccattacg
    11941 cgtcagaccg acgaattgac cggtctgtct tcgctggtgg ttctggattc cgcagaacgt
    12001 accgcaggtg gtaaagatct gcgtccggca ctgaaaatcg ttgatgctca gggtaacgat
    12061 gtactgatcc cgggtaccga tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt
    12121 cagctggaag atggcgtaca gatcagctct ggtgacaccc tggcgcgtat tccgcaggaa
    12181 tccggcggta ccaaggacat caccggtggt ctgccgcgcg ttgcggatct gttcgaagca
    12241 cgtcgtccga aagagccggc aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa
    12301 gaaaccaaag gtaaacgtcg tctggttatc accccggtag acggtagcga tccgtacgaa
    12361 gagatgattc caaaatggcg tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt
    12421 gacgtaattt ccgacggtcc ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat
    12481 gctgttactc gttacatcgt taacgaagta caggacgtat accgtctgca gggcgttaag
    12541 attaacgata aacacatcga agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt
    12601 aacgcgggca gctccgactt cctggaaggt gaacaggttg aatactctcg cgtcaagatc
    12661 gcaaaccgcg aactggaagc gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg
    12721 ggtatcacca aagcgtctct ggcaaccgag tccttcatct ccgcggcatc gttccaggag
    12781 accactcgcg tgctgaccga agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg
    12841 aaagagaacg ttatcgtggg tcgtctgatc ccggcaggta ccggttacgc gtaccaccag
    12901 gatcgtatgc gtcgccgtgc tgtgggtgaa gctccggctg caccgcaggt gactgcagaa
    12961 gacgcatctg ccagcctggc agaactgctg aacgcaggtc tgggcggttc tgataacgag
    13021 taatcgttaa tccgcaaata acgtaaaaac ccgcttcggc gggttttttt atgggtgtta
    13081 ttcagagggc aattcttcac ggaattttta agaaaggcag ggcgaaacga ggaagaagct
    13141 tttcgccctg gacaacattc ctgctgacgg gactaccata aaaaaacaca acgctttcaa
    13201 caaccttgcg tttttcatct tccgtagcgg ctcggaataa atttacgttc ttacattctg
    13261 tctcatggcc tttgcgaggc gcgtcccaga tagctgtccc agtctttcca taccggctgc
    13321 aaaccctgag cagttaacgc ggcagcaacc gcttccggtc tgcgatcgtc gtgcggtgag
    13381 aactgttcca gctcggggtg attgtcggca tagccacctg gctgcgtttt cgaaaaagcg
    13441 ctgacgttat taattgccag cggaataacg cgatcgcgaa accacggtga ttcccgcgtg
    13501 gagagtgaca gttcaatctc cggtgcaagc agccggaagg cgcagatggt ttgcactaac
    13561 tggcgttcat ccataatcga cgcaggctca atgccgccag tacatggacg caggcgtggg
    13621 aaggagatag agtagcggct ttgccagtaa tgctgttgca gccatagcaa atgttctgca
    13681 accatatagc agtcaacgcg ccagctgtcg gaaaggccaa ttagcgcgcc gaggcctatc
    13741 ttatcaatcc ccgcacggcc cagccgatcc ggcgtttcca gccgccagaa gaagtcctgt
    13801 tttttgcctt tcagatgatg gcgggcataa gtcgcctcgt gatatgtctc ctgataaacc
    13861 atcacgccat ccagaccaag ttgctttaac tcggcgtatt ccgtctccgc cagcggctgt
    13921 acttccatct gtagtgaaga gaactgttca cgcagggcag ggagatgacg acgaaagtaa
    13981 tccatcccca ctttcgcctg atgttcacca gtgactaaaa gcagatgttc aaagcccatc
    14041 tcccgtatag cggcgctttc cctggcaata tccgcttcat ccagcgtttt gcgcttgatg
    14101 cggttgctca tagagaatcc gcagtacgtg cagtcgttag cgcaaagatt ggaaagataa
    14161 agcgggacgt agaagctgac gacgttgcca aatcgctgac gggtcagacg ctgcgcccgt
    14221 tgggccagtg gttccagata gccactggcg gctggcgata acagcgccat catatcgtcg
    14281 cgggtgagtt gcgagacatt tagcgcccgc tctacgtcag cagccgtttt gccgttgata
    14341 cgcaggcgga tgttgtccca gtccagttgt cgccagcgat cgctgaaggt tttcatgccg
    14401 atgcctccag aaatccggtc agcgggctgg tggcatgagc aaaatgactg cggctgcccg
    14461 gtccggactg ccgtgccagc aggcctgctt ctactgccag acgaaatgcc ttcgccatgt
    14521 tgacgggatc gtccgcgacg gcaatcgccg tattcactaa caccgcgtcg gcccccattt
    14581 ccagcgcctg cgcggcatgg ctgggaacgc cgatgccagc atcgacaacc accggaaccg
    14641 tggcctgctg gataataatc tccagcatgg cgcgggtttc cagtccctga ttcgagccaa
    14701 tcggcgcgcc gagcggcatc accgctgcac agccgacttc ttccagacgt ttacacaata
    14761 ccggatcggc cccgcagtaa ggcagcacga caaatccctg ttgtaccagc gtttcggcgg
    14821 ctttcagggt ttcgatggga tcgggcaaca gccagcgggc gtcagggtga atctctaatt
    14881 ttagccagtt tgtgcctaat gcttcacgtg ccagatgggc ggcgaaaatg gcttcttccg
    14941 ctgttttcgc cccggaagta ttcggcagca gggtcacacc cgccgcgata agcggttcga
    15001 ggatagcgtc gttgtgctgg cgcaagtcga cacgtttcat cgccagcgtc accagctggc
    15061 tgccggaagc gcggatcgcc tccaccatca gttgtgaaga agcgaatttc ccggtgccgg
    15121 taaacagatg tgaatcaaac gttttgtccg caatacgtaa catctcaacc ccctgcaata
    15181 acctgaaaaa gcaggatctg gtcgccatcc tgcacgatat gttgcgccca ttgatcacgc
    15241 gggacgattt gctgattaat cgccagagcc gcgcccgctt gtcgttggtc gagttgctcc
    15301 agtagttcgt gaacagtttg cccggcggcg cactgcatcg gttgatcgtt aaacaggatc
    15361 tgcattgctt cctccgcata ccgggcaacc actggcgcgg cgcaacgcca ggctgcgcca
    15421 ctggctagac ttaccgtcga acagtcggag ttctcccgca ggcgtctcta taccgcttaa
    15481 taacttaatg gcttccagtg cctgcaaagt gcccataacc ccgaccaccg ggccaaccac
    15541 gcccgccgtg cggcagttgc gttctggctc ctggttatct ggccacaggc agcggtaaca
    15601 cccctgctcc cagggcggcg tcagtaccat caactgaccg ccaaatccga ccgcgctggc
    15661 ggtgataagc ggtgtgttga gtgccacgca ggcggcatta atctcctggc gagtcgccat
    15721 attgtcggta cagtcgagca ccacatcggc ttgtgcaacc gcatctttta acgcctcacc
    15781 cgttaaccgt tgttgtaatg ctgtcagttg aatatcggga ttcaactgtg tcagtcgctg
    15841 atggctgacc tgcgatttcg ggcgatcgat atcttcagtg gtaaagagga tttgtcgttg
    15901 cagattgctt aaatgcacat cgtcgtcatc tgccagtacc agcgtcccga cgccagcgcc
    15961 cgccaggtat agcgcagcag gtgtacccag cccgccaaga ccgataatca gcacctggct
    16021 gtcgagcagt ttttgctgcc cgtcaagagc gatatcgtcg agcaggattt ggcggctata
    16081 acgcataaag tcacggtcat tcatcgccaa ctcctgcaat ttccagcaac tgtgccgttg
    16141 ccaaacgcca gtctgcggct tgagtaatgg cgctgacaac ggcgatactg ccgacacccg
    16201 tcgccattac cgctggcgcg cgcggcagac tgataccgcc aatcgccacg gtgggataat
    16261 ccgccaggcg ctcaacatgc cgtgccagct gttccagccc ctgcggtgca gaaggcatct
    16321 gtttggtttg cgtcgggaac acatgtccca gcgcgatata agaaggacgt gctgccagcg
    16381 cgacgtcgat ttccatatca tcatgtgtcg aaacgcccag ccgcagacca gccgcgcgga
    16441 tggcgctgag atcggtggct tgcaaatctt cctgccccag atggacgcca tatgcctgat
    16501 gcttgatcgc cagccgccag taatcgttga taaacaatcg cgcgttatag cggcggccca
    16561 gcgcaattgc cgccacgaca tcggcttcca cctcttcatc gcgccgatct ttgatgcgta
    16621 gctggagagt acgcacgcct gcatccaaca gacgttcgat ccactgtacg ctgtccacca
    16681 ccgggtacag tcctaaacga aaaggtacag gaggaaaatc aggctgatac atcacgcttc
    16741 ctccttacgc aggtagattt ctccgcctct ggcgcggaag ttctccgaca tatccgccat
    16801 tcccacttca atggtttgcg cggcggcgta atcacgcact tcctggctga ttttcatcga
    16861 gcagaatttc ggcccacaca tggagcaaaa atgggcgact ttaccggact cttgcggcag
    16921 ggtttcatcg tgataagcgc gggcggtaaa cgggtcgagg gccagattaa actggtcttc
    16981 ccagcgaaat tcgaagcggg ctttcgacat ggcgttatcg cgaatttgcg cgcccggatg
    17041 ccctttcgcc aggtcagcgg cgtgggcggc aatcttatag gtgataagcc cctgcttaac
    17101 atcttcttta ttaggcagac ccagatgctc ttttggcgtt acgtaacaga gcatcgcgca
    17161 gccaaaccag ccaatcatcg ccgcaccaat ccccgacgtg aagtggtcat aacccggcgc
    17221 gatatcggta gttagcggcc ccagagtgta aaacggcgct tcgtggcagt gctctaactc
    17281 ctcggtcata ttgcggcgga tcatctgcat cggcacgtgg cctgggcctt caatcatcac
    17341 ctgtacgtca tattcccagg cgattttggt cagttcaccc agcgtatgca gctcggcaaa
    17401 ttgcgcttca tcgttggcgt cctgaacaga accggggcgc agaccgtcgc ccagcgacag
    17461 cgaaacgtca taagcggcac agatttcaca aatttcgcgg aagtgttgat agaggaaatt
    17521 ttcctgatga tgggagaggc accatttcgc cataatcgaa ccgccgcgag agacgatacc
    17581 agtcaggcgt ttcgcggtca tcggcacata gcgcagcagt acgcccgcat ggatagtgaa
    17641 gtaatccaca ccttgctcgg cctgttccag cagcgtgtcg cggaacgctt cccaggtaag
    17701 atcttcggcg atcccgttaa ccttctccag cgcctggtag atcggcactg taccgatcgg
    17761 caccgggctg ttacgcaaaa tccactcgcg ggtttcgtga atatagcgac cggtggagag
    17821 atccatcacc gtatccgctc cccagcgcgt ggaccatacc agcttttcca cttcttcttc
    17881 gatggaagag gtgaccgccg agttgccgat attggcgtta acttttacca ggaaattgcg
    17941 accaataatc atcggctccg attccggatg attaatgttg gccgggataa tcgcgcgtcc
    18001 ggcagcaact tcatcacgga caaattccgc agtgatattt tccggcagac gtgcgccaaa
    18061 gctcattccc ggatgctggt ggcgtaaaac ttcgctacgt atgcgctcgc gacccatatt
    18121 ctcgcgaatg gcgatgaatt ccatttcggg cgtgatgatg ccccggcggg cgtagtgcag
    18181 ttgggtgacg cgacgtcctg ctttggcgcg ctttggggtt aatacgccgc taaaacgtag
    18241 ctcgtcgagg ccatcatctg ccagccgcgc tttagtgtaa tcggaactgc gaacggtaag
    18301 ttcttcggta tcgccgcgcg catcgatcca cggctgacgt agttttgcca gcccttgctg
    18361 cacgttaatg gcgatctgtg gatcgccata cgggccggag gtgtcgtaga ccggaatcgc
    18421 ttcgttttct tcaaactgcg gctgttcttt gctaccgcca attagcgtcg ggctaagctg
    18481 gatctcacgc atcggcacgc gcacgccggg ttgtgtgcca gtgatataaa tgcgttttga
    18541 gttgggaaag gcggtgcctt ccagggtgtc gataaaatgt tgggcctggg cgcgttgttc
    18601 gcggcgggtc agttttgttg cagacatagc tcattccaaa aagttaagga cgtggcttgt
    18661 cagacgacgg atgaagcaag agacgatcgc cgcaaaggcg atgcgatagc agattaactc
    18721 ttgttccctt cgcaggtatt agcctgatca ggttccgcgg atcccgaata aacggtctca
    18781 gccagttaag gcactccgac aagaaattag ccccacaaac ggggcgttga atgtaaatta
    18841 cgcgttaaca gcgcagaact caagcaggat gtttgacgcg ggcggcgtca agcaccagca
    18901 gaatcagctt atcttccagc acaaagcgcg cttccagcgc ctcgccgatg tcagataaaa
    18961 cctgttgaaa ctcaaggtaa ttatcatgat cgatggcggt ttccaggctg gaatcgtagt
    19021 aatccataat ctgttgggta ttggcttcga gttgcggcca aatcttcgcg gcgcgcgcga
    19081 gttgcccgtt gccttccagc ttatgaagaa tgcgctcata aatactgaaa tgcccggcag
    19141 acaagtaatc gaccaggctc tgacaaaaat catcaagggc tttttcgttt agcctcatgt
    19201 acgattcttt gccaggctta atgccaacca gattgtagta agccacgagc agatgcttac
    19261 gtacatgtag ccagcgatca accagtttgt tacttcctct gacgcgctcc gtcaggttat
    19321 cgagctggtt aagcatgatt gactccgcaa gtttgtattc aaaaactgct cagtgagaaa
    19381 tgtaaaaacc atgttaaaca tgccagtgat gcaaaggtag tgcaagagct atggatcgta
    19441 taattgaaaa attagatcac ggctggtggg tcgtcagcca tgaacaaaaa ttatggttgc
    19501 cgaagggaga attgccatat ggcgaagcgg caaatttcga tcttgtgggt cagcgcgcac
    19561 tacaaatcgg cgaatggcag ggggaacctg tttggttaat acaacagcag cgtcgtcacg
    19621 atatggggtc ggtacgtcag gtcattgatc tcgatgttgg gctgtttcaa ctggccggac
    19681 gtggcgtgca actggcggag ttttaccgat cgcataaata ctgtggttac tgcgggcatg
    19741 aaatgtatcc aagcaaaacc gaatgggcga tgctgtgcag ccattgccgt gagcgttact
    19801 acccgcaaat cgccccctgc attattgttg ccatccgtcg cgatgattcg atcctccttg
    19861 cccagcatac ccgccatcgc aacggtgtcc atacggtact tgccggattc gtcgaagtgg
    19921 gcgaaaccct cgagcaggcg gtcgcgcggg aagtgatgga agagagcgga attaaagtta
    19981 aaaacttgcg ttatgtgact tcccagccgt ggccgtttcc tcagtcttta atgaccgcgt
    20041 ttatggcgga atatgacagc ggcgacatcg tgatcgaccc gaaagaattg ctcgaggcga
    20101 actggtatcg ctatgacgat ttgccgttac tcccgccgcc cggcaccgta gcgcgccgtc
    20161 tgatagaaga tacggtggcg atgtgtcggg cagagtatga gtgatgatac actgaccgcc
    20221 tgacgcacta aggaacagcc aaaatgaccg aacttaaaaa cgatcgttat ctgcgggcgc
    20281 tgctgcgcca gcccgttgat gtcactccag tatggatgat gcgccaggcg ggtcgctatc
    20341 taccggaata taaagccacg cgcgcccagg cgggcgattt tatgtcgctg tgcaaaaacg
    20401 ccgagctggc gtgcgaagtg actttgcaac cgctgcgtcg ctacccgctg gatgcggcga
    20461 tcctcttttc cgatatcctc accgtgccgg acgcgatggg gttagggctc tattttgaag
    20521 ccggagaagg tccgcgtttt acctcgccag tcacctgcaa agccgacgtc gataaactgc
    20581 caattccgga cccggaagat gagctgggtt acgtgatgaa cgcggtgcgt accattcgtc
    20641 gcgaactgaa aggcgaagtg ccgctgattg gtttttccgg cagcccgtgg acgctggcga
    20701 cctatatggt ggaaggcggc agcagcaaag ccttcaccgt gatcaaaaaa atgatgtatg
    20761 ccgatccgca ggcgctgcac gcgctactcg ataaactggc gaaaagcgtc actttgtatc
    20821 tgaatgcgca gattaaagcc ggtgctcagg cagtgatgat tttcgacacc tggggcggcg
    20881 tgcttaccgg gcgcgattat caacagttct cgctttatta catgcataaa attgttgatg
    20941 gtttactgcg tgaaaacgac ggtcgccgcg taccggtcac gctgtttacc aaaggcggcg
    21001 gacagtggct ggaagccatg gcagaaaccg gttgcgatgc gctgggcctc gactggacaa
    21061 cggacatcgc cgatgcgcgc cgtcgcgtgg gcaataaagt cgcgttgcag ggcaatatgg
    21121 atccgtcgat gctgtacgcg ccgccagccc gcattgaaga agaagtagcg actatacttg
    21181 caggtttcgg tcacggtgaa ggtcatgtct ttaaccttgg tcacggcatt catcaggatg
    21241 tgccgccaga acatgctggc gtgtttgtgg aggcagtgca tcggttatct gaacagtatc
    21301 accgctaaga gcctggtcca tcaggctatt ttatttgcca ttttgaggcc gggcagtgct
    21361 caaaatcctc acgtactacg tgtacgctcc ggtttttccg cgctgtccgg cctcaaactg
    21421 ccttcaacaa ttacgcctgg tggaccaggc tctaaggagc gattatggat ctcgcgtcat
    21481 tacgcgctca acaaatcgaa ctggcttctt ctgtgatccg cgaggatcga ctcgataaag
    21541 atccgccgga tctgatcgcc ggagccgatg tcggatttga gcagggcgga gaagtgacgc
    21601 gagcggcgat ggtgctgctg aaatacccct cgcttgagct ggtggagtat aaagtggccc
    21661 gcatcgccac caccatgcct tacattccag gttttctctc cttccgcgaa tatcctgcgc
    21721 tgctggcagc gtgggagatg ctgtcgcaaa agccggattt agtgtttgtc gatggtcacg
    21781 ggatctcgca tcctcgccgt cttggcgtcg ccagccactt tggtttgctg gtggatgtgc
    21841 caacgatagg cgtggcgaaa aaacggctct gcggtaaatt cgaaccgctc tccagtgaac
    21901 cgggcgcgct ggcaccgctg atggataaag gcgagcagct ggcctgggtc tggcgcagca
    21961 aagcgcgctg taacccgttg tttatcgcta ccggccatcg ggtcagcgta gacagcgcgc
    22021 tggcgtgggt acaacgctgc atgaaaggct atcgtctgcc ggagccaacg cgctgggcgg
    22081 acgcggtggc ctcggaacgt ccggcgttcg tgcgctatac agcaaatcag ccctaattca
    22141 ggtaaactgc ggataatttc cgtatttgag aactcaacat gttacaaaac ccaattcatc
    22201 tgcgtctgga gcgcctggaa agctggcagc acgtcacttt tatggcctgc ttatgcgaac
    22261 gcatgtaccc caattacgcc gtattctgtc agcaaactgg ttttggtgat gggcaaattt
    22321 accgccgtat tctcgatctc atctgggaaa cgctgaccgt caaagacgca aaagtaaatt
    22381 tcgacagcca actggagaaa tttgaagaag cgattccttc agccgacgat ttcgatctgt
    22441 acggcgttta tccggcaatc gatgcctgcg tggcgttaag tgaactggtc cattcgcgtt
    22501 tgagtggtga aacgctcgaa cacgcggtgg aagtgagtaa gacctccatc acgaccgttg
    22561 cgatgctgga aatgactcag gctggtcgcg aaatgagcga tgaagagctc aaagaaaacc
    22621 cagctgtaga gcaagaatgg gacattcagt gggaaatatt ccgactttta gctgagtgcg
    22681 aagaacgcga tatcgagctg ataaaaggcc ttagggcaga cctgcgtgag gcgggtgaga
    22741 gcaatattgg tataattttt cagcaataag accagaaaac gtgatttaac gcctgatttg
    22801 tcgtacctgg agtcttccct ttcgcccccc gtctggtcta catttggggg gcgaaaaaaa
    22861 gtggctatcg gtgcgtgtat gcaggagagt gctattctgg catttccgtc gcactcgatg
    22921 cttagcaagc gataaacaca ttgtaaggat aacttatgaa caagactcaa ctgattgatg
    22981 taattgcaga gaaagcagaa ctgtccaaaa cccaggctaa agctgctctg gagtccactc
    23041 tggctgcaat tactgagtct ctgaaagaag gcgatgctgt acaactggtt ggtttcggta
    23101 ccttcaaagt gaaccaccgc gctgagcgta ctggccgcaa cccgcagacc ggtaaagaaa
    23161 tcaaaattgc cgcagctaac gtaccggcat ttgtttctgg caaggcactg aaagacgcag
    23221 ttaagtaaga ttgcgtggca gtgaacagtt ttaacgaagg ggtggtttca ccccttttgt
    23281 ctttctggcg tcgatcattg atgctggcag gtgcgctgtt tctcactgcc tgtagccaca
    23341 actcttcact tcctcccttt accgccagtg gatttgctga agaccagggc gcggtacgca
    23401 tctggcgaaa agacagcggc gataatgtgc atctgcttgc cgtgtttagc ccgtggcgca
    23461 gtggcgatac caccacgcga gagtatcgct ggcagggcga taaccttacg ctcatcaata
    23521 tcaatgttta cagcaaaccg ccggtgaaca ttcgcgcgcg ttttgacgat cgcggcgatc
    23581 tgagctttat gcaacgtgaa tccgatgggg aaaagcagca gctttctaac gaccaaatcg
    23641 atttataccg ttatcgtgct gatcagatcc gccagattag cgatgcctta cgtcagggaa
    23701 gagtcgtgct gcgccagggg cgctggcatg cgatggaaca gaccgtgacc acctgcgaag
    23761 ggcaaaccat taaacctgat ttagattcgc aggcgatagc gcatatcgag cgccgccaga
    23821 gccgctcttc tgttgatgtc agcgtggcat ggctggaagc gcccgaaggt tcgcaattac
    23881 tgttagtggc aaactctgat ttctgtcgct ggcaacccaa cgagaaaacg ttctgattta
    23941 ccagtggccc atacccatat gactgccacc accgcagccg ccgtagccca tgcccattcc
    24001 ggtaccgcgc ggaatacccg cttcagccat cgcaatatct cgtttcaccc gtaattcatc
    24061 taacgactgg tgcaaattct ccatctcttt ggcgaccgcg ttaattttgc tgctatccgg
    24121 tgggttcgcg gctaacaggg cgttgtattc ataacgcttc gtcaccagtt gctgttgcag
    24181 tgcgctgctt tgagcgtaaa agtcattatg gattttctgc cacgctgtct gttgttcgct
    24241 ggtcaaaggc gcggcatttt gctgccacat accgtgtccg ccgtgagcaa atgccgatgt
    24301 cgatcccatc gccattgctg aaagcgccat cattaccagg gcaattttcg tgttccgttt
    24361 catggttaat cctccagtgg ttgtcttact tcgggtattg catcttccgt gccaacgatg
    24421 aaacgctgat atgacgggta atctggcatg ataaacgagt aaaaatgact cgcctgctgc
    24481 gggtagcgag tcatttttac tcattgaaac ttaagccttt gtgttacagc gcagggtaag
    24541 cgctgataaa agatggcatg atttctgctg tcagaaaggg atgagcaggc aaagaagaag
    24601 atgcgtttta tgcaacgttc taaagactcc ttagctaaat ggttaagcgc gatcctcccc
    24661 gtggtcattg ttgggctggt ggggctgttt gcggtgacag tgattcgcga ttacgggcgc
    24721 gagactgcag ccgccagaca aacgctgctg gaaaaaggca gtgtacttat ccgtgctctc
    24781 gaatccggct cgcgcgtcgg catggggatg cgcatgcatc atgcgcagca gcaggcatta
    24841 ctggaagaaa tggccgggca gcctggtgta cgttggtttg cggtcacgga tgaacaagga
    24901 acaatcgtga tgcatagcaa ctccggcatg gtgggaaaac agctttattc cccgcaggaa
    24961 atgcagcagt tacatccggg agatgaagaa gcgtggcggc ggatcgatag cgcagacggc
    25021 gagcctgttc tggaaattta tcgccagttt caaccgatgt ttgctgctgg aatgcaccgg
    25081 atgcgccata tgcagcagta tgccgcgaca ccacaagcaa tttttatcgc tttcgacgcc
    25141 agtaacattg tgagtgccga agatcgtgag cagagaaaca ccctgattat cctcttcgcc
    25201 ctggcgacgg tcttgctggc aagcgtgttg tcattcttct ggtatcgccg atatctgcgc
    25261 tcgggccagc tgttgcagga tgaaatgaag cgcaaagaga agctggtggc actggggcat
    25321 cttgcggcag gcgttgccca cgaaatccgt aacccacttt cctcgattaa agggctggcg
    25381 aaatactttg ccgaacgcgc gccagcaggg ggagaagcgc atcaactggc gcaggtgatg
    25441 gcgaaagaag ctgaccgttt aaaccgcgtg gtaagcgagt tgctggaact ggttaagcca
    25501 acgcatctgg ctttgcaggc ggtggaactc aacacgctga ttagccactc attacagctg
    25561 gtaagccagg atgcaaacag ccgggagatc cagttacgct ttaccgccaa cgacacatta
    25621 ccggaaattc aggccgatcc ggacaggctg actcaggtcc tgttgaatct ctatctcaac
    25681 gctattcagg cgattggtca gcatggcgtg attagcgtga cggccagcga aagcggcgcg
    25741 ggcgtgaaaa tcagcgttac cgacagcggt aagggaattg cggcggatca gcttgaagcc
    25801 atcttcaccc cgtatttcac cacgaaggcc gaaggcaccg gactggggct ggcggtcgtg
    25861 cataatattg ttgaacaaca cggtggtaca attcaggtcg taagccagga gggaaaaggc
    25921 gcaacgttca ccctctggct tccggtcact attacgcgta aggacccaca aggatgacgc
    25981 acgataatat cgatattctt gtggtggatg atgacattag ccactgcact attttgcagg
    26041 ctttgctgcg cggctggggc tataacgtcg cgctggcgaa cagcgggcga caggcgctgg
    26101 agcaggtgcg ggaacaggtt tttgatcttg tgctttgcga tgtgcgaatg gcagagatgg
    26161 acggcattgc cacgctaaaa gagatcaaag cgttaaatcc ggcgattccg gtactgatta
    26221 tgaccgcgta ctccagcgtc gagacggcgg tagaagcgtt gaaaaccggg gcgctggatt
    26281 atctcatcaa gccgctggat ttcgataacc tgcaggcgac gctggaaaag gcgctcgcac
    26341 atacgcacag tattgatgct gaaacgcctg tggtgactgc cagccagttc ggtatggtcg
    26401 gtaaaagccc ggcgatgcaa cacctgctca gtgaaatcgc cctcgtcgcg ccatcggaag
    26461 ccacggtgct gatccacggc gattccggca ccggtaaaga gctggtcgca agggcgattc
    26521 acgccagtag cgcacggagt gaaaaaccac tggtaacgct caactgtgcg gcactcaacg
    26581 aatccttgct ggaatctgaa ttgttcggtc acgaaaaagg ggcgtttacc ggagccgata
    26641 aacgccggga agggcgcttt gttgaggcgg acggcggcac gctgtttctc gatgaaattg
    26701 gcgatatctc gccgatgatg caggttcgtc tgctgcgtgc gattcaggag cgcgaagttc
    26761 agcgtgtcgg cagcaaccag accatctcgg ttgatgtccg gctgattgcg gcgactcatc
    26821 gcgatcttgc cgcagaggtg aatgccgggc gttttcgcca ggatctctac tatcgcctga
    26881 acgtggtggc gattgaagtt ccgtcgctgc gtcagcggcg ggaagatatt cctctgctgg
    26941 caaaccattt tcttcagcgc tttgccgagc gtaatcgtaa ggcggtaaaa ggttttacgc
    27001 cccaggcgat ggatctgctg attcactacg actggccggg aaatattcgt gaactggaaa
    27061 acacggtgga acgggcggtg gtgctgctga ccggggaata tatttccgaa cgcgagctgc
    27121 cgctggcgat tgcgagtacg ccgatcccgc tggtacaaag tcaggatatt cagccgctgg
    27181 tggaagtcga aaaagaggtg attcttgcgg cactggagaa aacgggcggc aacaaaaccg
    27241 aagccgcccg tcagttaggg atcacgcgca aaacgctctt ggcaaaactg tcgcgttagt
    27301 tctgctcgcg ttcgatagcg cgccagccga tatctttccg gcagaagcag tcgtcccaat
    27361 ggatatcggt cattaaggca taagcgcgtt tctgcgcttc tgccacggta tgacccagcg
    27421 cggtgacgca cagtacgcgc ccgccgctgg tcactacctg ctcgtcatcc gccagtttcg
    27481 tgcccgcgtg gaacactttg ccgtcagaca cttcttccag cggcaggccg tggatcacat
    27541 cgccggtgtg gtaatcgccc ggatatccac ccgcagccat caccacgccg agagaagcgc
    27601 gttcatccca ctcggacgtt ttctcgtcca gcttgccttc acaggccgcc aggcaaagct
    27661 caaccagatc ggacttcatg cgcagcataa tcggctgggt ttctggatcg ccaaagcggc
    27721 agttaaattc aataaccttc ggattgccct gtttgtcgat catcaggccc gcgtagagaa
    27781 aaccggtgta ggtgttgcct tccgccgcca tgcctttcac ggttggccag atgatacgtt
    27841 ccatcgtgcg ctgatgaact tcatcggtca ccactggcgc aggggagtaa gcgcccatcc
    27901 cgccggtgtt cggcccggta tctttatcgc ctacgcgttt gtgatcctgg ctggtggcca
    27961 tcggcagcac atgctcaccg tccaccatca cgataaagct cgcttcttcg ccgtcgagga
    28021 actcttcgat aacgatgcga tgccccgcgt cgccaaaagc gttgcccgcc agcatatcgt
    28081 gaacagccgc ttccgcttct tcctgcgtca tcgccacgat aacgcctttc ccggcagcca
    28141 gaccgtcagc tttaatgacg attggcgcgc ctttctcacg cagatacgcc agcgcaggtt
    28201 ctacctcggt gaagttctgg tattccgccg tagggatctg atggcgggcc aggaaatcct
    28261 tggtaaacgc tttcgagcct tccagctgcg cggcacctgc ggttgggccg aagattttca
    28321 gcccggcggc gcggaaggta tccaccacgc ctttcaccag cggcgcttcc gggccgacga
    28381 tggttagatc gatcttttcg ttttgcgcga aatccagcag cgccgggata tcggttacgc
    28441 cgatagcgac gttttgcagt gcaggctcca gcgcagtgcc tgcgttaccc ggagcaacaa
    28501 aaacagtttc aaccagcggc gactgggccg ctttccaggc cagcgcgtgc tcgcgcccgc
    28561 cgttaccaat cactaatact ttcatctatt gctccattaa tggcggaagt ggcgcatgtc
    28621 ggtgaagagc attgcaatac cgtgctcgtc ggcggcggca atcacttcgt catcacggat
    28681 agaaccgcca ggctggatta cgcaggtcac gcccgcagcg gcggcggcat caataccgtc
    28741 gcggaacggg aagaacgcgt cagaagccat cgaggaacct ttcacttcca gaccttcatc
    28801 ggcagcttta ataccggcga tttttgcgga gtacacgcgg ctcatctggc ccgcgccaat
    28861 cccgatggtc atattgtttt tggcatagac gatagcattg gatttcacga acttcgccac
    28921 tttccagcag aacagcgcat cacgcagttc ctgttcggtc ggctgacgtt tggtgacgac
    28981 gcgcagttct tctgcaccga ccatccccag gtctcgatcc tgaaccagca gaccgccgtt
    29041 cacgcgtttg aaatcaaggc ccggaacacg ctcgccccac tgaccgcagg tcaggacgcg
    29101 cacgttctgt ttggcggcgg tgattttcag ggcttcttcg ctggcggatg gcgcaatgat
    29161 cacttcaaca aactgacgag aaatgatggc ctgtgcggtt tccgcatcca gctcgcggtt
    29221 aaaggcgata atgccgccga acgcggaggt tgggtcggtt ttgtacgcgc gatcgtaagc
    29281 atcaagaatg gaatggccga tagccacgcc gcaagggttg gcgtgcttca caatcacaca
    29341 tgccggctcg gcgaactctt tcacgcactc cagcgccgca tcggtatcgg cgatgttgtt
    29401 ataagagagg gctttaccct gaacctgggt tgcggtagca acggaggctt ctttcacatt
    29461 ctcttctata tagaaggcag cctgctggtg gctgttctcg ccgtaacgca tatcctgctt
    29521 cttaatgaag ttcaggttca gcgtgcgtgg gaagcgaccg gcggcttctt tgctttcacc
    29581 gtggtaagcc ggaaccatgc tgccgaagta gttggcaatc atgctgtcgt aggcggcggt
    29641 gtgttcgaag gctttaatgg caaggtcaaa gcgagtttcc agagtcaggg agccttcatt
    29701 ggcatctatc tctttaataa tggcgtcgta gtcgctgctc ttcaccacga tggcgacatc
    29761 tttatggttc ttggcggcag agcgcaccat cgttgggccg ccgatatcga tgttctcaac
    29821 cgcatcttcc agcgagcaac cttcacgggc aacggtctgg gcgaacggat acaggttaac
    29881 aaccaccata tcgataggct ggatctgatg ttcttccata atggcatcgt cctggccgcg
    29941 acgacccaga atgccaccat gtactttcgg atgcagggtc ttcacgcgtc catccatcat
    30001 ctctgggaaa ccggtgtaat cggaaacttc ggttaccggc agaccttttt ctgctaacag
    30061 acgggcagtg ccccctgtag acagcagctc cacaccgcgt gcggaaagtg cctgggcgaa
    30121 ttcgacgata ccggctttgt cagaaacact gagcagagcg cggcggactg gacgacgttg
    30181 ttgcatggta aatcccctgg atttgactat tacagagagc gttagctgaa tttttcgcga
    30241 aaaactcagc taacgcccct aacggggcat ccttattttt cgcccgcatt gtaacgaaaa
    30301 cgtttgcgca acgctcgcga atttttctct ttcaatggtg atcacaattt tgacagtggt
    30361 tgccgtggga aaaatacaga aattacattg atgatggtgg ataactctgt gcgtaaaaag
    30421 gtataaagcg gggttttgct ggggaatgca gcagtcagtc atttttctgc aatttttcta
    30481 ttgcggcctg cggagaactc cctataatgc gcctccatcg acacggcgga tgtgaatcac
    30541 ttcacacaaa cagccggttc ggttgaagag aaaaaaccct gaaattcagg gttgactctg
    30601 aaagaggaaa gcgtagtata cgccacctcg cgacagtgag ctgaaagccg cgtcgc
//
LOCUS       DAPHCV010000058        30423 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.058, whole genome shotgun sequence.
ACCESSION   DAPHCV010000058 DAPHCV010000000
VERSION     DAPHCV010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.058"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(122..685)
                     /gene="fimA"
                     /locus_tag="RH033_002556"
     CDS             complement(122..685)
                     /gene="fimA"
                     /locus_tag="RH033_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDV2991729.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            complement(1119..1385)
                     /locus_tag="RH033_002557"
                     /pseudo
     CDS             complement(1119..1385)
                     /locus_tag="RH033_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(1661..2977)
                     /gene="hipA"
                     /locus_tag="RH033_002558"
     CDS             complement(1661..2977)
                     /gene="hipA"
                     /locus_tag="RH033_002558"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HDV2991730.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEQLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPQVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHRRLSREWY"
     gene            complement(2977..3243)
                     /gene="hipB"
                     /locus_tag="RH033_002559"
     CDS             complement(2977..3243)
                     /gene="hipB"
                     /locus_tag="RH033_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HDV2991731.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            complement(3466..4797)
                     /locus_tag="RH033_002560"
                     /pseudo
     CDS             complement(3466..4797)
                     /locus_tag="RH033_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899537.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand"
     gene            complement(4941..6533)
                     /gene="lsrK"
                     /locus_tag="RH033_002561"
     CDS             complement(4941..6533)
                     /gene="lsrK"
                     /locus_tag="RH033_002561"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HDV2991732.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENKVYRTTGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSHAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPTSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(6612..7565)
                     /gene="lsrR"
                     /locus_tag="RH033_002562"
     CDS             complement(6612..7565)
                     /gene="lsrR"
                     /locus_tag="RH033_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HDV2991733.1"
                     /translation="MTINDSVISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAECSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            7814..9349
                     /gene="lsrA"
                     /locus_tag="RH033_002563"
     CDS             7814..9349
                     /gene="lsrA"
                     /locus_tag="RH033_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HDV2991734.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYTRLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSIQKMNNLLVALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSQ
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPALREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKDINRLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEIAHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            9343..10371
                     /gene="lsrC"
                     /locus_tag="RH033_002564"
     CDS             9343..10371
                     /gene="lsrC"
                     /locus_tag="RH033_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HDV2991735.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYVTGDNLQGARQLGVRTESIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGMVLLAVLVFDGRLRCALERNLRRQKYARFMMPPPSVKPAS
                     SGKKREAA"
     gene            10371..11363
                     /gene="lsrD"
                     /locus_tag="RH033_002565"
     CDS             10371..11363
                     /gene="lsrD"
                     /locus_tag="RH033_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HDV2991736.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVAVYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            11375..12397
                     /gene="lsrB"
                     /locus_tag="RH033_002566"
     CDS             11375..12397
                     /gene="lsrB"
                     /locus_tag="RH033_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HDV2991737.1"
                     /translation="MTLHRFKKIALLSALGIAVISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            12424..13299
                     /gene="lsrF"
                     /locus_tag="RH033_002567"
     CDS             12424..13299
                     /gene="lsrF"
                     /locus_tag="RH033_002567"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HDV2991738.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKAGIPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVDKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            13323..13613
                     /gene="lsrG"
                     /locus_tag="RH033_002568"
     CDS             13323..13613
                     /gene="lsrG"
                     /locus_tag="RH033_002568"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HDV2991739.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            13670..14428
                     /gene="tam"
                     /locus_tag="RH033_002569"
     CDS             13670..14428
                     /gene="tam"
                     /locus_tag="RH033_002569"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HDV2991740.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIKYVADLGCGPGN
                     STTLLHQRWPAARITGIDSSPAMIAEARSTLPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNSHGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQSIIDWVTATGLRPWLQDLTESEQQHFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(14432..15346)
                     /locus_tag="RH033_002570"
     CDS             complement(14432..15346)
                     /locus_tag="RH033_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HDV2991741.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISVVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(15543..16994)
                     /gene="uxaB"
                     /locus_tag="RH033_002571"
     CDS             complement(15543..16994)
                     /gene="uxaB"
                     /locus_tag="RH033_002571"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDV2991742.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGIQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILKKGMREAVRPLC"
     gene            complement(17222..18640)
                     /locus_tag="RH033_002572"
     CDS             complement(17222..18640)
                     /locus_tag="RH033_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDV2991743.1"
                     /translation="MHVQPISTFRLFQEGHLLRNSIAIFVLTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVARDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFAQHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGCASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(18779..19138)
                     /locus_tag="RH033_002573"
     CDS             complement(18779..19138)
                     /locus_tag="RH033_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HDV2991744.1"
                     /translation="MQSLDPLFVRLSRSKFRSRFRLGVKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGEALSEEQQR
                     YIVTVIYHWLVIQMNQP"
     gene            complement(19138..20064)
                     /gene="glsB"
                     /locus_tag="RH033_002574"
     CDS             complement(19138..20064)
                     /gene="glsB"
                     /locus_tag="RH033_002574"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HDV2991745.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(20128..21516)
                     /gene="sad"
                     /locus_tag="RH033_002575"
     CDS             complement(20128..21516)
                     /gene="sad"
                     /locus_tag="RH033_002575"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HDV2991746.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGVDDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSLMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKIAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRV"
     gene            21617..22492
                     /locus_tag="RH033_002576"
     CDS             21617..22492
                     /locus_tag="RH033_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2991747.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSIVAGDEPQGLFSLGSLESTAAV
                     RIPAMLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVTRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQS"
     gene            22576..22659
                     /gene="yneK"
                     /locus_tag="RH033_002577"
                     /pseudo
     CDS             22576..22659
                     /gene="yneK"
                     /locus_tag="RH033_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            22837..24027
                     /gene="ydeA"
                     /locus_tag="RH033_002578"
     CDS             22837..24027
                     /gene="ydeA"
                     /locus_tag="RH033_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HDV2991748.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(24052..24717)
                     /gene="marC"
                     /locus_tag="RH033_002579"
     CDS             complement(24052..24717)
                     /gene="marC"
                     /locus_tag="RH033_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HDV2991749.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            24929..25363
                     /gene="marR"
                     /locus_tag="RH033_002580"
     CDS             24929..25363
                     /gene="marR"
                     /locus_tag="RH033_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HDV2991750.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            25384..25767
                     /gene="marA"
                     /locus_tag="RH033_002581"
     CDS             25384..25767
                     /gene="marA"
                     /locus_tag="RH033_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HDV2991751.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQSESRFLHPLNHYNS"
     gene            25799..26017
                     /gene="marB"
                     /locus_tag="RH033_002582"
     CDS             25799..26017
                     /gene="marB"
                     /locus_tag="RH033_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HDV2991752.1"
                     /translation="MKPLLSAIAPAFILFSTQGIAEQTTQQVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(26048..26947)
                     /gene="eamA"
                     /locus_tag="RH033_002583"
     CDS             complement(26048..26947)
                     /gene="eamA"
                     /locus_tag="RH033_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HDV2991753.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSATMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFFGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            27142..28329
                     /gene="ydeE"
                     /locus_tag="RH033_002584"
     CDS             27142..28329
                     /gene="ydeE"
                     /locus_tag="RH033_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HDV2991754.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSAWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGIVLTSLPPFSLFIILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            28456..28551
                     /gene="mgtS"
                     /locus_tag="RH033_002585"
     CDS             28456..28551
                     /gene="mgtS"
                     /locus_tag="RH033_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HDV2991755.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            complement(28770..29660)
                     /gene="dgcZ"
                     /locus_tag="RH033_002586"
     CDS             complement(28770..29660)
                     /gene="dgcZ"
                     /locus_tag="RH033_002586"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HDV2991756.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYELCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGIRICQLVDNHAIKHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(29915..30307)
                     /gene="ydeI"
                     /locus_tag="RH033_002587"
     CDS             complement(29915..30307)
                     /gene="ydeI"
                     /locus_tag="RH033_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HDV2991757.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT         7697 a         7693 c         7238 g         7795 t
ORIGIN      
        1 aaataaaaac cattcctcat taaaaatgag atgaacatag gtatattgct cgggtgtgcg
       61 tagccatgcc acacacaccc tttttcaatt tcagatgacg tatagccacc ttaagattga
      121 attatagata ttgaactttg aaggtcgcat cagcgtttgc agtaccagct actgactgcc
      181 cgagagcaat ataacgagcc tggaacggca atatattcgt gccatcaatc aagtcagttt
      241 tagcgctaaa agtggcacca tcaagtatta atacctcccc cgtacggtcc aaaatttgaa
      301 taccgacatt ttgggcagag cctgccgctg aactctgcag cgctaacgta tcgtcattac
      361 tggtgacagt agtacccagg aatgcaacag ctgcattact ggaaacattg gtatcacaat
      421 cattcagctt gatattgaag ccaacggcgg agctgaggtc acccgcttta gccagacgag
      481 aagaacggac ctgacccagc tcaaccgttt ggttcattga ttctgaatca atcgcacaag
      541 cggcatttac cacttcacct ttaaaatgaa taacaccgcc attaacagtg gtcgttactg
      601 attcatcacc ctcggctgcg cttaccgcag cagacgacat cgccaataca gaaacgacaa
      661 tcataccgac atgtttgagt ttcataattc tccaaaattt atatttaagt agctgaataa
      721 agagagttaa caattaagtc attctgttat tgcttataaa acaagaatca ttcttaattg
      781 gtagttgccc tgagaatata gtttccaggg ttattacatt tcacgaagaa cgataatatc
      841 tttaaattac aaggacacct aatcacaaca agtttattgc agcacttgtt gtagagagta
      901 tttgcaaaga cattttatgt cgctaaataa catgacgatt tcattatgca tgttatttat
      961 aaaccgtgac taaacagaat attattgcga acaagggaag tgtcaggaaa ggtcaagagc
     1021 gaccaatggt ttatatggct ataaatcatt agccatagtt aatgtttgcc tggtgtaact
     1081 aacacggaat tgctctaaac agataagtgt agggtttatt aaagctgacc gacagacttt
     1141 cttaacctgg taatatctct cttaggtgac tctccaaaca tccgcgaata ttcccgactg
     1201 aaatgggata agctttcgta accgacagca taggctgcag tggtgacatc gtaatgttca
     1261 ttcagcatca gtcgcctggc ttcattgagg cttagccatt tccgataccg gagtggactc
     1321 attcccgcaa gctgtctgaa atgttgatgg aaagaggggg tgttcatttg cacgcggttt
     1381 gccagcctga ctcttcaacg ctgagaagat gcggcaaacg gcaaacctgc ataccagcca
     1441 gcctatatcc ggtatgcaca ggccaattat gaaagaggag aggctttatg ttctggaacc
     1501 ttgaagtaat aacgtcgacc ggaaaatcac tctgcatcat cactcagatt caatttaaca
     1561 taatatacat tatgcgcacc aacataaacc aagggagaat ccagccgttg gcggtcatga
     1621 ttgtcctgct cattaacaac ggccacaccc catatcttac ttaataccat tctcggctta
     1681 accgacggtg caacctcaac acattggttt caactgccgt caccacgttc tccggaaaat
     1741 ctgtcggtaa tgaagtcttc acgttatcca gtgctgccgg aatcattctg gcaaagtcac
     1801 tcaggatttc atgcatctgc acttgcggaa atttcagcac ctttgctgtc gccagaaaat
     1861 gtcgcggata aattttatcg attgccgttt ttttgccttt ggatgcgtta agccccattg
     1921 ccagtttgag atcgctgatg tgtattcctg caccacccaa taccggaaat gctgaaatga
     1981 tgtcgtaaaa tggcgtgagt cgataactgc cgccagcctg aataaatacg gagaagtttt
     2041 ttgcatggcc atccgttgcg ccaatcaacc actggaacac ctgaaacttc ataaaatcat
     2101 agcgatcttt cagcgcctcg ctggacccca tcaaaaaagc catgatccgc gcgatgcctg
     2161 ggcctccatc tgattcatat ttcaccgatg aaggtaaacc gaatgtctga cacatatcct
     2221 cctgtggcaa gcgaagtaaa accgttcgct cagcattcca acgcctgtca aaacgttcga
     2281 ccgctaacgc gcgcacattt cccgctttaa tgatttctgc gtccggcaca ttcaacccaa
     2341 gttctttcgc cagcagcaga cagtaatact cattatcaac gctttggctg agatcgagcg
     2401 tcgcattggg ctgcctgatt tcgccaatcg gtaatttaat gatgtgcgtc gtcggcgtta
     2461 ttcctttcgg aatgcaccag tcattgccta ttctgagcag cgctgtcttc tcctgcgcgc
     2521 cagcaaccga gatgcgaaag tcattttctt ctctaatcat gcccagaggg atatctgctt
     2581 tataagccgt taatacttct tcaagcctgg cttcagtgag ctgttcccat gccattatcg
     2641 ggcgcgttat ggtttcattt tcgggtaata acgttacggc accaacgctg tctcgcccta
     2701 tttctgacaa taaatcaaac ggttgtctgg atttagcatg ataacgttta acgatccgat
     2761 cacgtacaat cgggctatcg ggtaacaggt tatcgaagaa gttaaatacg gcatcagagg
     2821 tgatattccc cctctgcaat ggcagcgaaa gtgacaacgg tctggcataa cggctggcta
     2881 accactccgg tgcatactta aaggtgtgcg cgccattggc taacttcgtt aactcgccta
     2941 cccgctggtt gttcatccaa gtgacaagtt taggcattac cactccagat cttgctgttc
     3001 tgttgattct ggcgaggcat ttttcgtgtc gcatagcgtc attgagagtt caagcgactg
     3061 taaaatctta aaaaaggtcg tcagcgtggt attgtcaggg ttgttttcga aattggaaat
     3121 cgtcgcctgc ttaataccaa tttttttcgc cagctcgctc tgcgtccagc cattttgctg
     3181 gcgaaccagt ttcattgcat tcgccaattg cgttgggcta tagatcttct gaaagctcat
     3241 catgtcatac gtccacagca aatttatccg cttaagggga tattatcagt tttatccttt
     3301 agtgaggata agtcaatctg gaaacacatc ttatccgctc tacgggataa acactgtgat
     3361 atccgcgatc gcggataaaa aggaggattt aaaagtcgtt tgaagtcggc aatattaagc
     3421 cgcatgcatc ttcgacatgc ggctattccc ttgggtatcg ccttattgcc ccacaaagcg
     3481 gctattatcg ccatccagct ctacgtctgt cctggcggtt gcgctcacga tacccttccc
     3541 gcttatgcta ttacgcagtt cgccggtcac gttttttagc gttaacagac cgtcattggc
     3601 aatatttcct ctgcctaacc cctgcgtatt atcgaggctg acttccgcat ttgggctaat
     3661 gctggttaac gcattgtagc gtgcattaag cccctcgata gccagtgttc cccctgtaac
     3721 attcaggttt ccgccacctg tcagttcgcc ctgactgatc ccacctttat tcaccgtcag
     3781 cgacccctct ttaaacaaaa cagtcgaacc catttgcccg gtgaatgtct ctaccgtctg
     3841 ggtcgatcca ttaagatcga cgatggccgc gttgctgatg ttcagttccc gggtgttgcc
     3901 cagcgcgcca tccgcatcgg tacgtaatgt ccccatctga acgtaggttg ccccctgata
     3961 gtcgttctga ccgttggaaa gcgaaacctg tcgtaccgta ttgattgcca gatccccttc
     4021 gccaccgatt tttgccgaca tatcggccgt tgcgccataa gctccgccat gttcggctaa
     4081 cgtcagcttt tgcccaccat ggatgttcag cgctttcagc ccatagttaa catacaaacc
     4141 atcaccaggt gcggtcgtga ggcggaatcc ataatcaccc tcggcgacca ctgttccgtt
     4201 ttgtgcaatg tgtaacgttt tgtccgcttt cacctcgtcg ccctgtaaat cacgtaacgt
     4261 taatgagcca cccgacccaa taaccgtttg cgccttcacc agttgaacgc cgacatgact
     4321 atcatcgtgt tccagcaaat tgagcgtcgt cagagggtta ttcgccatgg gatcattcca
     4381 cggtttaggc acgtcgataa gcacgtcgcc cgtcccgttt acctgatagt tacggccttt
     4441 ccaggtgtag tcacccgcgc cgacaaccag cgtatcgacg ctgatatatc cctccgcaag
     4501 cgtcgcagca ggtatatccg tatcgaacgt gagcgtaccg ccattcatgg ctagtccacc
     4561 aatggattgc tctcccactt ttaccgatgt ggtattttca ctgtcagact gcagcatcgc
     4621 gtcagtaagc gcagcggtgt tgtcgcgttc cagtgcgaag gtactggctt tcagttgggc
     4681 aacaccggcg aattcagtcc ctgctgcatg ggtaaagcca aacattttgt cggaggatga
     4741 cagctgcact cgcatcaagc catcgccttt gagcgcattg atcagcgtga atagcaattt
     4801 ccccttgaat atagtactgg tgatggaacg atgaatctgc aagctggcat ttaacaagcc
     4861 agctctaaaa gaagggaaat gagaatacca atactcaaaa actaacagcc acggtcatca
     4921 tggtgtggct gtcaatgaaa ctataaccca ggcgctttcc ataacgacgt cgtcagtcca
     4981 tgatcaacca gccccagctg atcctgataa actgcctgcc acttatcgcg tgaatcctga
     5041 taaagttcat gcttttccgg gtctggtgtg tgcgtccgtt cccagcgaac caggcgttct
     5101 ccggtttctg ccattgatga aaaaattccg gcaccgacgc cagctgcaat ggcacatcct
     5161 aatgcggtgg cttctttgac taccggaata ttgacgggta atcccgagac atcagcgaga
     5221 atttgactcc ataatttccc ttttgaacct ccgccagcaa aaaccagcga ggtgggatga
     5281 atattcgaga aatcagcaat ttgctgtaag ttacacgctg atacaatcgc cgcattttct
     5341 tccagcgcac ggaacaatgt cgctttgtta catttatccg ggtcaatgga caagttaata
     5401 aaggaaggcg cagcgtgata ccaggtttta aagcgcattc tgtcggagaa tatcggcatt
     5461 acgccccacg acccaggcgg cacccgactg gccatctctt ccagcagcgt ataggtgtcg
     5521 atgcctaaac gttcggcaat cagtttttct tcggcacaga aagcatcgcg gaaccagcgc
     5581 atggttagtc cggtaaaaaa gcttatagat tcagcttgta ccatgccagg aataacatgg
     5641 ggattaacgc gcacgttcat ttctgggtct gtcaccggcg cggctaagtt tacaacttgc
     5701 tgccagaatg tgccgccaag aaccgcggtt tgtgccggac gtacaacgcc taaccccagg
     5761 caaccaagct gcacgtcacc tcctccaacg accaccggag tgcccgcctt cagaccgcag
     5821 agttctgccg cgtgtgaact taccacgccc agcaatgtgc cggtttcttt gacaggagaa
     5881 agaatatcgg cccgcagtcc ggccatatcc agcaatgcag gtttccagtc acgggtggtt
     5941 agatcaagaa gtcccgtggt gccagcgtta gagggatcca ccgccagttc gccgctgagc
     6001 atataggcca gccagtcgct gatcatggtg atggttgatg cctgacggta gatatcagaa
     6061 cgatggtgcg ccagccaaag taatctgggg atggcgctta aagccagcgt ttgcccggtc
     6121 gttcgataaa ctttgttttc aaaggtattg ttgtgcagtt ctttaagttc gctaacttcg
     6181 cgcgccgctc tggcatccac attggcgcag gcccagatcg gtgttccctc attgttatat
     6241 aaaacaatac cttcgcgcat cgaacatgcc gaaacggcag cgatatactc cggggctatg
     6301 ccggcgttgt gcagcgcctg gcgcatacac tcacacgcca gttgccagtt tttgttgaga
     6361 tcaaattcca tagaaccagg aacgtccggt actgccaggt gccgccactc cgcctgtccc
     6421 acggctattt gattgccttc caggtcgaat atcacagccc gaatacttcc ggtacctgca
     6481 tccagcgcca tcaggtagta ctttgattca gaaggggtaa agagtcgagc cattagatta
     6541 tcctcggctt atgcgttctg tctgttcctc tgtacgttct ccatcattcc cggtaataag
     6601 gtcatgcaaa tttaactacg taaaatcgcc gctgctgtat cctgatcggt aaccagtgcg
     6661 ttgatataac cgcctttcat tgcagcggca atggcttcgg ctttattttc tccccctgcc
     6721 acgccaaccc ggacgggtat ggtcttcagc gcgcttaaag gtaagccaat cagttcgtta
     6781 tgtattttga tatccgtgac aacgtcacct tttgcatcaa aaaagtagcc taaaatgtcg
     6841 ccaaccgccc cttttcgacc aatcattaac tgttcgccct ggctgatata gccggagcga
     6901 atgattgttg cgtcgtcttg ttgactcaca gccccaatgc cgacaatcgc cacatccgct
     6961 gcttgcgcgg ctagcagaac atctttgacg caattttcat tttttagcgt acgggcaatg
     7021 tcagcggagg atgcccgcaa cggagccgga ataatattca cgctgcactc cgcgttaagc
     7081 tgcccgatac ccgtcatata agaaccgact ccaccggaga gcgtgaccag gcgaatttgc
     7141 tgtgacgaaa taaaaccact taagcgttgc agcgtattca tggttgcctc gccaaaacca
     7201 atcgccagca tctgttgtgg ttgaagtaaa ctcatcaaca tatgcgccgc gcctatcccc
     7261 agtcgcccgc cgacatcagc atccgcaagc cctgggatca cccggacatg ttgcagcgaa
     7321 aactgacgac gtaattgtgt ttcatattcc agacagcctt caaagcgtga attaatctgt
     7381 acgcgaataa tgccggattg atgccctttc tccagtaatc gcgacacttt caaacgggtc
     7441 aggccgagac gatcgctgat ctcactctgg gtcagcccgt cgtggtagta aaaccacgca
     7501 atccgcgcga cctgttcttc ttcacacatt ccctgttctg aaattaccga atcgttgatt
     7561 gtcataattc attcttcact ttgaacatac ttaaatcttt aatgcaattg ttcagttctt
     7621 gcgcatttat atctgtgatg gcaaccacag tttgactcta tgagcatgaa caaacgcaac
     7681 cgtgaaaatc aaaatagcat aaattgtgat ctattcgtcg gaaatatgtg caatgtccac
     7741 ctaaggttat gaacaaatta aaagcagaaa tacatttgtt caaaactcac ctgcaaaact
     7801 gaacggggga aatatgcaaa cgagtgatac ccgcgcgtta ccgctacttt gcgcccgctc
     7861 ggtttataaa cagtattcag gggttaatgt cctgaaaggc atcgatttta cgttgcatca
     7921 gggggaggtc cacgcactgc tcggcggcaa tggtgccggt aaatcgacgt taatgaagat
     7981 tattgccggt attacccctg ctgatagcgg tacgctggag attgggggca acaactacac
     8041 cagattaacg ccagttcatg ctcatcagct gggcatttat ctcgttcccc aggaaccgct
     8101 gctttttcca agcctgtcga taaaagaaaa catcctgttt ggtctggcaa aaaaacaact
     8161 ctccatacag aaaatgaata acttgctggt ggcgctgggc tgccagtttg atctgcatag
     8221 tctggcagga tcgctggatg tcgccgatcg ccaaatggtg gaaatcctcc gtgggctgat
     8281 gcgcgactcg cagattctga tcctcgatga acctaccgcc tcgcttaccc ctgcggaaac
     8341 cgaacgcttg tttagtcgct tgcaagagct gcttgctact ggcgtgggta ttgtttttat
     8401 ctcgcataag ctgccggaaa ttcgccagat tgccgatcgg attagcgtga tgcgcgacgg
     8461 aaccatcgcc ttaagcggca aaaccagcga actgtctacc gacgacatta ttcaggccat
     8521 cacgccagcg ctacgggaaa aatcgctctc tgccagccaa aaattatggc tggaattacc
     8581 tggcaaccgc ccacaacatg ccgccggaac gccggtgctg acactggaaa atctgaccgg
     8641 cgaaggtttc aggaatgtca gcctgacgct caatgccgga gaaattctgg gcctggctgg
     8701 actggtggga gccggacgca cagaactggc cgagacgctc tatggtctgc gtactttgcg
     8761 tggcggacgc attatgctga atggtaaaga tatcaataga ttatccaccg gagaacgttt
     8821 actgcgcggt ctggtttatc tgccggaaga tcgccagtca tccggactta atctcgatgc
     8881 ttcactggcg tggaacgtct gcgcccttac tcataacctt cgtggattct gggcgaaaac
     8941 cgcgaaagat aatgccaccc tggaacgata tcgccgggcg ctgaatatta aatttaacca
     9001 accggaacaa gctgcgcgga ccttatccgg cggcaaccag caaaaaatcc tgattgccaa
     9061 atgtctggaa gcttcgccgc aagttttgat tgtcgatgag ccgacgcgcg gcgtggatgt
     9121 ctcggcgcgt aatgatatct accaactgtt gcgcagcatc gccgcacaaa atgtggctgt
     9181 gctgcttatc tcctctgatc tggaagagat cgaactgatg gcagatcgcg tgtatgtgat
     9241 gcatcagggc gaaattgccc actctgcact gaccggacgc gatattaatg tcgagaccat
     9301 tatgcgcgtt gccttcggag atagtcagcg tcaggaggcg tcatgctgaa atttattcag
     9361 aacaaccgtg aaatcacggc actgctggcg gtggtgctgc tgtttgtatt acccggtttt
     9421 ctcgaccgtc agtatttaag tgtgcaaacg ctgaccatgg tttatagcag cgcgcaaatc
     9481 ctgatcctgc tggcaatggg cgcgacgctg gtaatgctta cgcgcaatat tgatgtttca
     9541 gtgggttcga ttaccggaat gtgcgcggtg ctgttgggga tgttactgaa cgcggggtat
     9601 tcactacctg ttgcttgtgt cgcgacttta ctgcttggtt tgctcgcggg atttttcaac
     9661 ggtgtcctgg tcgcgtggct aaagatccct gccattgttg ccacccttgg cacgttaggg
     9721 ttgtacagag gcatcatgtt gctgtggact ggcggtaaat ggattgaggg attacccgcc
     9781 gaactgaaac aactctccgc cccgctgctg tttggcgttt ccgcgattgg ttggttgacg
     9841 ataattctgg tggcatttat ggcctggctg ctggcaaaga cggcgtttgg acgcagtttt
     9901 tatgtcacgg gcgataattt acagggcgct cgtcaactgg gcgttcgtac ggaatccatt
     9961 cgcattgtgg cattttcgtt gaacggctgc atggcggcac tggcgggaat tgtgtttgct
    10021 tcgcagattg gttttatccc caaccagacc ggtaccgggc tggagatgaa agcaattgca
    10081 gcctgcgtgc tgggcggcat tagtttgctc ggcggttccg gcgcgatcat cggtgcggta
    10141 ctcggcgcat ggttcctgac gcagatcgat agtgtactgg tgctgttgcg cattccggca
    10201 tggtggaatg attttatcgc gggcatggtt ctgctggcgg tgctggtgtt tgatggacgc
    10261 ctgcgttgtg cgctggaacg taatctgcgg cggcaaaaat atgcccgctt tatgatgcca
    10321 ccgccatccg ttaaacccgc ttcgtcaggt aaaaaacggg aggccgcgta atgcgtattc
    10381 gctacggttg ggaactggct cttgccgcac tgctcgttat tgagattgtt gcatttggtg
    10441 caattaaccc gcggatgtta gatctcaata tgttgctgtt cagcaccagt gactttatct
    10501 gcattggcat tgtcgcccta ccgctgacga tggtgattgt cagtggcggg atcgatattt
    10561 cgtttggttc gaccatcggc ctctgcgcca ttgcattggg cgtactgttt caaagtggtg
    10621 tgccgatgcc gctggcgatt cttctgacct tactgctcgg cgcattgtgc gggctgatca
    10681 acgccggatt aattatctat accaaagtta acccgctggt gattacgctt ggcacgctgt
    10741 atctgtttgc cggaagcgcc ctgctgcttt ccgggatggc cggagcgacg gggtacgaag
    10801 gtattggtgg attcccgatg gcgtttacag atttcgctaa cctggatgtg ctggggctcc
    10861 ccgttccgct gattatcttc ctgatatgtc tcctcgtttt ctggctctgg ctgcataaaa
    10921 cccatgccgg acgtaacgtg tttttaattg ggcaaagccc gcgtgtggcg gtttatagcg
    10981 cgattccagt taaccgcacc ttgtgtgcgc tctatgccat gacggggctg gcgtctgcgg
    11041 tcgccgctgt gctgctggta tcgtattttg gttcagcacg ttccgatctc ggtgcgtcgt
    11101 ttctgatgcc cgccatcacc gccgtagtgc tcggcggtgc caatatttat ggtggttctg
    11161 gttccattat cggcaccgcc atagcggttt tattagtggg atatttgcaa caaggtttgc
    11221 aaatggcagg agtgccaaat caggtgtcca gcgccctttc cggtgcgcta cttatcgtcg
    11281 ttgtcgtagg tcgttccgtt agcctgcatc gccagcaaat taaagagtgg ctggcgcgtc
    11341 gggccaataa cccattgcca taaaggatat cttcatgaca cttcatcgct ttaagaaaat
    11401 cgccttactt agcgcgcttg gcattgccgt aatctctatg aatgtgcagg cggcagagcg
    11461 tattgcattt attcccaaac tggttggcgt gggatttttt accagcggtg gcaacggcgc
    11521 acaacaagcg ggtaaagagt tgggcgttga tgtgacctac gacgggccga cagaacccag
    11581 tgtttctggt caggtacagt tgattaataa cttcgtcaat caggggtata acgccattat
    11641 cgtttctgcg gtttcgcctg atggcttgtg tccggcactg aaacgcgcca tgcaacgtgg
    11701 tgtgagagtg ctgacctggg actctgatac taaaccggag tgccgctctt actacattaa
    11761 tcagggaacg cccgcccagt tgggaggtat gttggtggat atggcggcgc gtcaggtgaa
    11821 taaagacaaa gccaaagtcg cgtttttcta ctcaagcccc accgttacgg accaaaacca
    11881 gtgggtgaaa gaagcgaaag cgaaaatcgc caaagagcat ccaggctggg aaattgtcac
    11941 tacgcagttt ggctataacg atgccactaa atcgttacaa accgcagaag gaatattaaa
    12001 agcctatagc gatctcgacg ccattatcgc ccccgatgcc aatgccctgc ccgctgccgc
    12061 acaagccgca gaaaacctga aaaatgacaa agttgcgatt gtcggattca gtacgccaaa
    12121 cgtgatgcgt ccatatgtgg aacgcggcac ggtgaaagaa tttggcctgt gggatgtggt
    12181 tcagcaaggc aaaatatcag tttatgtcgc ggatgcatta ttgaaaaaag gatcaatgaa
    12241 aacgggcgac aagctggata tccagggcgt aggtcaggtg gaagtctcgc caaacagcgt
    12301 tcagggctat gactacgaag ctgatggtaa tggcatcgta ctgttaccgg agcgcgtgat
    12361 attcaacaaa gagaatatcg gcaaatacga tttctgatgt gcattactta accggagtac
    12421 gttatggcag atttagatga tattaaagat ggtaaagatt ttcgtaccga tcaaccgcaa
    12481 caaaatatcc cttttaccct gaaaggttgc ggtgcactgg attggggaat gcagtcacgc
    12541 ttatcgcgga tatttaatcc gaaaacgggt aaaaccgtga tgctggcctt tgatcatggt
    12601 tattttcagg ggccgactac cggacttgaa cgcattgata taaatatcgc cccgctgttt
    12661 gaacatgccg atgtattaat gtgtacgcgc ggcattttgc gcagcgtagt cccccctgcg
    12721 accaataagc cggtggtact gcgggcgtca ggtgcgaact ctattctggc ggagttaagt
    12781 aatgaagccg tggcgttatc gatggatgat gccgtgcgcc tgaacagttg cgcggtggcg
    12841 gcgcaggttt atatcggcag cgaatatgaa catcagtcga tcaaaaatat tattcagctg
    12901 gtcgatgccg gaatgaaagc gggaataccg accatggccg tgactggtgt gggcaaagat
    12961 atggtgcgcg atcagcgtta tttctcgctc gcgactcgaa tcgccgctga aatgggggcg
    13021 cagattatca aaacttatta tgtcgataaa ggttttgaac ggattgttgc cggatgtccg
    13081 gtacccattg ttattgctgg cggtaaaaag ttaccggagc gcgaggcgct tgaaatgtgc
    13141 tggcaggcta tcgatcaggg cgcttctggt gtggatatgg ggcgtaatat tttccagtct
    13201 gaccatccgg tggcgatgat gaaagccgta caggcggtgg tacaccataa cgaaacggct
    13261 gatcgggcat atgaactcta tctgagtgaa aaacagtaac tgcggattta aggagaagaa
    13321 ttatgcacgt cacactggtt gaaattaacg ttcatgaaga caaggttgac gagtttatcg
    13381 aagtttttcg ccagaaccac ctgggctctg tacaggaaga aggcaatttg cgcttcgatg
    13441 tcttacagga cccggaagtg aattcgcgct tttatatcta cgaagcctat aaagatgaag
    13501 acgcagtggc attccataaa accacgcccc actacaaaac ctgtgtcgcg aaactggaat
    13561 ctttaatgac cgggccgcgt aaaaaacgtc tgttcaatgg gttgatgccg tgaggcgaat
    13621 ttatcagttt tatctacaat tggggtaacg cgctgacggg agtaaataaa tgtctgactg
    13681 gaacccctct ttatatctgc actttgccgc tgaacgatcg cgtccggcgg tggagctgct
    13741 tgccagagtg ccgctggaga atatcaaata cgttgccgat cttggctgtg gcccaggtaa
    13801 cagcaccacc ctgctgcatc aacgttggcc tgcggccagg ataacaggca tcgactcgtc
    13861 tccggcgatg attgctgaag cgcgcagtac tttgccagac tgccagttcg tggaagcaga
    13921 catccgcaac tggcaaccgg aacaggcact cgatctgatt tttgctaatg cctcactgca
    13981 atggctgccc gaccactacg aattgttccc tcatctggtt tctttactta attcgcatgg
    14041 tgtgttggca gtacagatgc cagataactg gctagagcca acccatgtgc tcatgcgcga
    14101 agtcgcctgg gaacaaaatt atccggatcg cgggcgtgag ccgctggctg gcgttcatgc
    14161 ttactacgat attctgagtg aagccggatg tgaggtcgat atctggcgaa ccacttacta
    14221 tcaccagatg ccgtcgcacc agtcgattat cgattgggtg accgccactg gattacgtcc
    14281 gtggttacag gatctgaccg agagcgaaca gcagcatttt cttacgcgct accatcagat
    14341 gctggaagag caatatcccc tccaggagaa cggacagata ttgctggcat ttccgcgtct
    14401 gtttattgtt gcccgccgta cggagtaatt atcacgtcag ctggtaatgg cgatcgggaa
    14461 gaattttcgc tggaatttct gcttcatcgt tcatctgtaa caggtcaatt tcaatagcgt
    14521 tgcagatggc atccagcggt aagtcattgt tttcagtacc gaagggatct tccagttctt
    14581 ccgccagaca atccagcgaa ataaaagtgt aggaaatcag cacagagata aaaggcgtca
    14641 tgtaatgcag gtccacgacc agcgcaaacg gcagcatgat acaaaacaga tacacggtgc
    14701 gatgcaaaat cagggtgtag gcaaagggaa ttggcgtata ggcaatgcgc tcgcatcctg
    14761 ccaggaccac tgaaatatca ttaagccgat cgttgaggct aataaacagg atatctgaaa
    14821 gttgtccatt gcgtcgctga accgccaacc actcccccat tattaacaag atgcggttag
    14881 ctggagagtt agaagccagt acacgctgaa gatcttcagt cttgagataa tgagccagca
    14941 cttccgcctg tggctgtttg cgtaatgtca tgcgtaaaca gtgggcgaag gcgatttgca
    15001 gccgggcaaa ctcccttaca cttgccgaat ccggcaatgt cgtttttacc tcgcgcagta
    15061 acgaccgcga ggcaatcatc aactgccccc aaagttttcg cgcttcaacg taacgggcgt
    15121 aaccggcatt attacgaaaa ccaagaaaaa tggcgatggc gacaccgaga atgctgaacg
    15181 gtgcgagggt gaatttaatg cccaaatgcg tgtaccaggg cagcatgaaa ataacagcga
    15241 tagaaaagag aaaattgagt agtaagcgcg aggatatctt ggataatact gaaccgtgcc
    15301 agacaaaaat acggcgcagc cagtgttgtt gtggacgaac aatcatggtt atcttcaggc
    15361 gtggaaaagt cgccctatta aacgtgatta cgatcacatt ctcaagacac tacttacaaa
    15421 ttacctacaa gcttacaaca ttaacaggtg ctgcatgtgg cacctgttaa tgataaaggc
    15481 gcataggatg ttgtaactaa tatagtgaaa taagaaaccc ggtcgaaacc gggttcagag
    15541 tattagcaca acggacgtac agcttcgcgc attccttttt tgagaatcgc atccaggtca
    15601 ttagccacct gctcgaccag accaggcact tgcgtcaggt cctgctccca gtggtctttt
    15661 tctgccaaca caatcgctac cagttcctgg ataccgataa cacggtcacg atgctggctc
    15721 cagagttgct gataacgttc cagccagtgt gcatcgtcct gtaccggata tgtttctcca
    15781 ttacgctcac cgcgataaaa cgcaatcaat gccgctaatg cgaaagtcag gcgcgccgga
    15841 agtgtgccgt ttgccttctg ccctgccagc agctgcggca ggatgcgtgt gcggaacttg
    15901 gtcataccgt tgagcgcgat agacagcagt tgatgcttga tgtacgggtt acggaaacgt
    15961 ccggtgactg cactggcgaa agactccagc tcatcgcgtg gcaaatccag taccggaata
    16021 atctcttcgt agatggcttt ttcaacgaat gcgcaaattt cagcatcgtt catcgcttca
    16081 cctacggtat ccagccctgc ctggaacgcc accggcacca gcgcggtgtg cgcaccgttg
    16141 aggattgcca ctttgcgctc tttatacggt ttaatatcgt caacaatcag cacgttgagt
    16201 ggatatttgt ccagacgcag ctcggtcgct aaggatttcg gcccctgaat cacaaacagg
    16261 taaaagtgtt cagcggtgtc gaggaaacca tcgtgataac ccagttcttc ttccagttta
    16321 gccacttcat cgcgcggata accggtaacg atacggtcca ccagcgtaga acagaagctg
    16381 ttagcctgat ccagccattg aataaatgct tctggcagtg cccactcttg cgcatagcgc
    16441 agcaccagtt cacgcaacgc gtcgccattg tagtcgatca actcacacgg gatgatgatc
    16501 caacctttat ccagcgcacc gttgaaatgg ctaaaacgtt cgaacagcag acgggtcagt
    16561 tttgccggat aacttaccgc tggcgcgtca tcgaatttat cgcccgcatg gtagctgata
    16621 cccgcttcgg tggtgttaga gaaaacaaag cgcatttccg ggttgtgcgc cagtttcagg
    16681 aattcatcgt attcactgta gacgctgatt tcacgattaa ccgagcgaat cagacgcgcg
    16741 tcgctgaccg cttcaccctt ctcattcagg ccacggataa tggtggtgta cagaccatcc
    16801 tgtgtgctca gtgacggtgg gaatgaagtt tcaatcggac gaacaacgac cacgccagaa
    16861 ttcagatcgg tgtgctcatt caggagatcg atttgccagt caacaaaggc gcgcaggaag
    16921 ttaccttcac caaactgaat gatacgttct ggatactgtg caccgggaaa atcgcgacga
    16981 tttagtgttt tcacaatggg ttcccttctg attagtcata caacctgttt gaattggtac
    17041 gacaggttag caaactttaa tacaccgaac ccctgttttg atcaactcct gatgattaat
    17101 gagcagtttt atgagaaaag tgtggcgcgg atcatggttt tatagaggaa ctacgccttt
    17161 tattcgctca ataaagtggt agaacacatt ccgttaaaat cgctgtgaat gaataaagat
    17221 gtcagacaac ttcctcaccg taacgcatag tgctggtacg gttgcgccca tctttcttcg
    17281 agcgatacag acatgtatcg gcttcgacca tcaatttatt aaagtcatcg gtcagcgttc
    17341 ggtacgatgc gcaaccacta ccgacgccaa tacttaccgt gagataaagc gttttttgtt
    17401 gccaggtgaa cggttgcagt tcaacgcctt tacggatttt ttccgccatt agcagaccat
    17461 cgacaggatt caccgatggc actgcaacag caaattcttc gccgcccatt cgcgccacca
    17521 gccccttatc accgacaatc ttctgaatat gctgggcaaa cacgcttaac actttatcgc
    17581 cacattcatg tccatagtta tcgttaatgc ttttgaaata gtcgatatca agcagcatga
    17641 cagtcagatg ttgtgtctgt ttcagcgatg gacttttcag cgcctcataa agaccggacc
    17701 gtgagtaaac ctgagtcaga aaatcaaagt cggctcgcag cgcaacttgc ttcattagcg
    17761 aattgatcgc tgccacgcta aaagaaacca taattgggca tatcgccatc gtagcaatac
    17821 cgagacgtgc ggagaacatt tgcggaatgg agaacggcga accgaccgag atatcaatca
    17881 ccgaatttgc caccagtacg atttccaccg caccggtgac aaaggtcaac agacatgtta
    17941 cctgcggcgt atagcgcacg gcacaccaga ttaatgcagg cagcgggaac gccagactcc
    18001 ccgctccgcc aatgactacc gaggcaataa ccgacacaat aagcgcgatg gcgggcatca
    18061 tctgttctgc tttaaagcgc ggcagcactc ccggaatagc caacgtcagc atacaaggca
    18121 cgatcaacac gcccgttgag aattgctcac tgaaccagtc cgcaagcaaa ggccagaaat
    18181 ccagactatc aatactgacc gaaccaatcg ccccgacaat agcgcataat aatgcggcaa
    18241 tcagacagta attaaatagc cgtaaggcac ttactggctc atacttattt tttcccagac
    18301 gcttatcccg cgcgaccagt aaggcgacgg taacaataaa catcatattg gagaaattga
    18361 tagccagtga aaccagcccc cattcggtgg ttatggcatc ataaaccagc atcgccacat
    18421 aactgatcgc atagtaatgc agtcgattaa gccagacata gcgggcaaac acccccgcca
    18481 ttacgccatt cagcggccag aaaagagaaa gttcgtgaac cagccgtaac tctgcaccaa
    18541 taaaatagaa cagcgtggtt agcacaaaaa tagcgatgct attacgtagc agatgacctt
    18601 cctggaacaa acggaaagtt gaaatgggct gtacatgcat taattatctg tccatacaat
    18661 tgcgggtggc aattatctca acgcaataag taaattctta gaaaggatag catgttcgct
    18721 gacaacggcg gcaactaaag atatcctggg aatgctattt tatcgatttc cgtaattatc
    18781 atggctgatt catctgtata accagccagt ggtagattac cgtcacaata taacgttgtt
    18841 gctcctcact taacgcttcc ccctgaggaa tgttatgcca cttagccaga caaccgcgac
    18901 aacaggtcgc ggtggcgtgt tgagcgataa aaaccggatg cccgcgcatg ggggtttgct
    18961 taccgtcgtt agccggtaat gccggagcaa gacgtttagc gacaaaatcc gccgcatgtt
    19021 gttcaatgac tggcgcgcct ttctccagac aatactgacg ctctttcaca cccaaacgaa
    19081 agcgagagcg aaattttgaa cgggataaac gcacgaagag tggatcgaga gactgcatta
    19141 ataaactgaa cgccctaatt gtttcgtcaa ttgttcaagg acggcaatgc ccgcaagcga
    19201 gttaccggca tcatccagtt ccggactcca gactgcgatg gccatttcat gcggaacaat
    19261 cgccacaata ccgccaccaa cgccagattt tgccggtagt cccacccgcc aggcaaactc
    19321 ccccgcgttc tggtacatac cgctggtcgc catcagcgcg ttaatttgcc gcgcctgcat
    19381 tggcgtcacc acaggttcat caatatgaat agctttcccc tgattcgcca gaaagacaaa
    19441 cgtccgggcc agctctacac agctcatttt cagagcgcag taatgaaagt agttttgcag
    19501 aacggttgtc acgtcatgat ggaaattgcc aaacgacttc atcagccagg cgatagccgc
    19561 atttcgcgcg gaatgttcaa attcggaacg cgctaccacc gtatcgtagg aaatatcaga
    19621 cacaccactt aagccgcgca cgacttccag catacgttgc cgaggtgcgc ttaatcgccc
    19681 ttgcaacata tcgcagacca ccagcgcacc ggcattaata aacggattac gcggaatacc
    19741 ctgctccatt tccagttgca ctaaagaatt gaatggtgat ccagacggat ctttgccgac
    19801 gcgttgccag atttcctctt cggagtaatg acgcatggcg acaacgagac tcagcacttt
    19861 ggaaatagac tgaatggaaa aacgttcttg cgcgtctccg gcctgaaaaa gctgtccgtc
    19921 aacggtacag atagcaatcc ccaatcggga accgtctacc gtagccagcg ccggaatata
    19981 atccgcgact ttaccctgac caatgagcgg ccgcacttgc cgcaagatgt tttctaaaat
    20041 tgcattatcc atggcgactg ccactttcta ctcctggacc gcaggtctga aaagacctgc
    20101 gagtatatca gagctgaata tatagcgtca gacccggtct ttccacaccg tctggatatt
    20161 acagaattcg tgtaagccaa aatgggaaag ctcacgccca aagccactct tcttcacgcc
    20221 accaaaggcg actcgcgcat cgctggcaca ataaccattg ataaacaccc caccgcattc
    20281 cagacgtgcc gccatctgtc tggcctgtgt ttcgtcggtg gtgaaaatgg tcgctgaaag
    20341 gccgaactca ctatcattag ccagttccag cgcatgttct gcatctttcg caatggtgat
    20401 tgccgcaacg gggccaaaca tttcttcccg aaacgcggtc atttctgggg taacattcgc
    20461 cagaaccgtt ggcggatagt agttacctgc cccagcaatc ttttcccctc ccagtaacaa
    20521 acgcgcaccc tgcgccagtg ttttttccac ctgatgatgc agctcatcac gtaaatcaaa
    20581 acgagccatt ggtccgagag cgttctcttc atcacgggga tcgcccattt tcaaggctgc
    20641 cgcagctgcc acaaaacgtt cggtaaatgc cgaagcaatt ccctcttcga tgataaagcg
    20701 ttttgccgct gcacaaacct gtccggtatt ctgataacgt ccggctaccg ccgctttcac
    20761 cgccagttcc agatcggcat cgttaagcac aataaacgga tccgaaccac ccagttccag
    20821 tacgcatttt ttcagtgccg ctccagcctg tgcgccaata gccgctccag cacgaacact
    20881 tccggtcact gtgacagcag caatgcgcga gtctttaatc atcagactga caccgtcgtt
    20941 gtcggcattc agccagccat atacgccttg tgggataccc gcatctttaa acacctgggc
    21001 aatgagctgt gcacagccca tcacattcgg cgcatgttta agtaagtagc cgttaccagc
    21061 aagaatgatg ggaacagcgc cacgcatcac ctgccataac ggaaaattcc acggcataat
    21121 cgccagaatc gtccccaacg gtcgatactc aataaccgcc tgctgatttt ccaccagcgt
    21181 aggttccgcc ttcagcattg ccggcccatg ttctgcatac cagtcacaca aattcgccga
    21241 tttcgccact tcagcgcgcg cctggttgat tggtttgccc atttcacggg tgatcatttg
    21301 cgccatttct tcgctacggg cgcgcagagc cttaccgata tcacgcagtt tttcagcacg
    21361 ataatctata tttgtctcgc gccagtcgcg aaagcctgct gccgccagct gaagtgcgtt
    21421 ttcgatatcg tcaacaccag cccacggcag cacagaaagt tgttcaccgg tggcaggatt
    21481 tatcgaaatt gcatgagttg ccggagtaat ggtcatcggg gtatctcctt tatgagtcat
    21541 ggtatgaaga tacgcagatt tactcttgct ttaaaatgaa taatattaag ccacttattc
    21601 acgaatcgag aatgttatgg atctgaccca actggagatg ttcaacgcgg ttgccgaagc
    21661 tggcagcatt acccaggctg cagcaaaagt gcatcgcgtg ccgtcgaatc tcactacccg
    21721 tctacgtcag ctggaaacag aactgggggt tgatctgttt attcgcgaga atcagcgttt
    21781 acgtctctcg cctgccgggc ataacttttt acgctatagc caacaaattc tcgcattagt
    21841 ggatgaagcg cggagcattg tcgctggcga tgagccgcaa ggtttatttt ctcttggttc
    21901 gctggaaagc accgctgcag tgcgcattcc ggccatgctg gcggaattta accgtcgtta
    21961 tcccaaaatt cagttttcac tttccaccgg cccttccggc actatgctgg aaggggtgct
    22021 ggagggaaaa ctgaatgcgg cgtttattga tgggcccatt aaccatactg ccattgacgg
    22081 gataccggtg taccgcgagg agctgatgat tgtcacgcca caaggacatg cgccagtaac
    22141 ccgtgccagt caggttaatg gcagtaacat ttatgccttc cgcgccaatt gttcgtatcg
    22201 tcgccacttc gagagctggt ttcatgctga cggtgccgct ccgggaacta ttcacgagat
    22261 ggagtcgtat cacgggatgt tggcctgtgt ggtcgcagga gcaggcattg cgcttattcc
    22321 acgctcaatg ctggaaagta tgccggggca tcaccaggtt gaagcgtggc cgttagctga
    22381 gcaatggcgt tggttaacaa cctggctggt ctggcgtcgc ggtgcgaaaa cgcgtccgct
    22441 tgaggcattt attcaactac tggatgtgtc tgactcggca aaacagagtt agtaatgagc
    22501 tatttttgat agttcttgcg gctaatatgc tctatatagt gatgctccga tgacttatga
    22561 ctatatgggg caaatatggt tacgccagta agcatcagca atttcatctc acttcctgat
    22621 gattttcccg ttcgtaatat tgcacctcag gtaaaagaag aacaggcccc cttcctgatc
    22681 gtcattaaaa tgtgatgtta acaactctct gatgcgtgac agaacaaccg tcagagagat
    22741 ggaaatgcga tccagatcac aaatgcattg tattcacatc attaaccgtt ttaagatcat
    22801 ttcatcactt tttcgcaact cacccgataa tctgttatga caacaaacac tgtttcccgc
    22861 aaagtagcgt ggctacgggt cgttacgctg gcagtcgccg ccttcatctt caacaccacc
    22921 gaatttgtcc cagttggcct gctctctgac attgcgcaga gttttcacat gcaaaccgct
    22981 caggtcggca ttatgttgac catttacgca tgggtagtag cgctaatgtc attgcctttt
    23041 atgttaatga ccagccaggt tgaacggcgc aaattactga tctgcctgtt tgtggtgttt
    23101 attgccagcc acgtactgtc gtttttatcg tggagtttta ccgttctggt gatcagtcgc
    23161 attggtgtgg cttttgcaca tgcgattttc tggtcgatta cggcgtctct ggcgatccgt
    23221 atggctccgg ccgggaagcg agcacaggca ttgagtttaa ttgccaccgg tactgcactg
    23281 gcgatggtct taggtttacc tctcgggcgc attgtggggc agtatttcgg ttggcgaatg
    23341 accttcttcg cgattggtat cggggcgctt atcacccttt tgtgcctgat taagttactt
    23401 cccttactgc ccagtgagca ttccggttcg ctgaaaagcc tcccgctatt attccgccgc
    23461 ccggcattga tgagcattta tttgttaact gtggtggttg tcaccgccca ttacacggca
    23521 tacagctata tcgagccttt tgtgcaaaac attgcgggat tcagcgccaa ctttgccacg
    23581 gcattactgt tattactcgg tggtgcgggc attattggca gcgtgatttt cggtaaactg
    23641 ggtaatcagt atgcgtctgc gttggtgagt acggcgattg cgttgttgct ggtgtgcctg
    23701 gcactgctgc tacctgcggc gaacagtgaa atacacctcg gggtgctgag tattttctgg
    23761 gggatagcga tgatgatcat cgggcttggt atgcaggtta aagtgctggc gttggcacca
    23821 gatgctaccg acgtcgcgat ggcgctattc tccgggatat ttaatattgg aatcggcgcg
    23881 ggtgcgttgg taggtaatca ggtcagtctg cactggtcaa tgtcgatgat tggttatgtg
    23941 ggcgcggtgc ctgcctttgc cgcgttaata tggtcaatca tcattttccg ccgctggcca
    24001 gtgacactcg aagaacagac gcaatagttg aaaggcccat ttgggccttt tttagtggta
    24061 cgttttaatg atttccagga tgccgttaat aataaactgc acccccatac ataccagcag
    24121 gaatcccatc agacgggaga tcgcttcaat gcccccctta cccaccagcc gcataattgc
    24181 gccagagctg cgtagacttc cccacaaaat aaccgccacc aggaaaaaga tcagcggcgg
    24241 cgcaaccatc agtacccaat cagcaaaggt tgaactctga cgcactgtgg acgccgagct
    24301 aataatcatc gctatggttc ccggaccggc ggtacttggc attgccagtg gtacaaaagc
    24361 aatattggcg ctgggttcat cttccagctc ttccgacttg cttttcgctt ccggtgaatc
    24421 aatcgctttc tgttgcggaa agagcatccg aaaaccgata aacgcgacga ttaagccgcc
    24481 tgcaattcgc agaccgggaa tcgaaatgcc aaatgtatcc atcaccagtt gcccggcgta
    24541 atacgccacc atcatgatgg caaatacgta caccgaggcc atcaacgact gacgattacg
    24601 ttcagcactg ttcatgttgc ctgccaggcc aagaaataac gcgacagttg ttaatgggtt
    24661 agctaacggc agtaacacca ccagccccag gcctattgct ttaaacaaat ctaacattgg
    24721 tggttgttat cctgtgtatc tggtttatca gcgaaaagta taaggggtaa acaacgataa
    24781 agtgtcactc tttagctaac cttgcatcgc attgaccaaa acttgaaccg atttagcaaa
    24841 acgtggcatc ggtcaattca ttcatttgac ttatacttgc ctgggcaata ttatcccttg
    24901 caactaatta cttgccaggg caactaatgt gaaaagtacc agcgatctgt tcaatgaaat
    24961 tattccattg ggtcgcttaa tccatatggt taatcagaag aaagatcgcc tgcttaacga
    25021 gtatctgtct ccgctggata ttaccgcggc acagtttaag gtgctctgct ctatccgctg
    25081 cgcggcgtgt attactccgg ttgaactgaa aaaggtattg tcggtcgacc tgggggcgct
    25141 gacccgtatg ctggatcgcc tggtctgtaa aggctgggtg gaaaggttgc cgaacccgaa
    25201 tgacaagcgc ggcgtactgg taaaacttac caccagcggc gcggcaatat gtgaacaatg
    25261 ccatcaatta gttggccagg acctgcatca agaattaaca aaaaacctga cggcggacga
    25321 agtggcaaca cttgagcatt tgcttaagaa agtcctgccg taaacaaaaa aagaggtatg
    25381 acgatgtcca gacgcaatac tgacgctatt accattcata gcattttgga ctggatcgag
    25441 gacaacctgg aatcgccact gtcactggag aaagtgtcag agcgttcggg ttactctaaa
    25501 tggcacctgc aacggatgtt taaaaaagaa accggtcatt cattaggcca atacatccgc
    25561 agccgtaaga tgacggaaat cgcgcaaaag ctgaaggaaa gtaacgagcc gatactctat
    25621 ctggcagaac gatatggctt cgagtcgcaa caaactctga cccgaacctt caaaaattac
    25681 tttgatgttc cgccgcataa ataccggatg accaatatgc agagcgaatc gcgcttttta
    25741 catccattaa atcattacaa cagctagttg ataacgtgac aacgtcactg aggcaatcat
    25801 gaaaccactt ttatccgcaa tagcacctgc gtttattctc ttttccacgc agggcattgc
    25861 ggaacaaacc acgcagcagg ttgttacttc ctgtgccaat gtcgtggttg ttcccccatc
    25921 gcaggaacaa ccaccgtttg atttaaatca catgggtact ggcagtgata agtcggatgc
    25981 gctcggcgtg ccctattata atcaacacgc tatgtagttt gttctggccc cgacatctcg
    26041 gggcttatta acttcccact tttaccgctt tacgccaccg caagccaaat acattgatat
    26101 acagcccggt cataatgagc accgcaccga aaaattgtag acccgttaag cgttcatcca
    26161 acaatagtgc cgcacttgcc agtcctacta cgggcaccag taacgataac ggtgcaaccc
    26221 gccaggtttc atagcgtccc agtaacgtcc cccagatccc ataaccaaca attgtcgcca
    26281 caaacgccag atacatcaga gacaagatgg tggtcatatc gatagtaacc agactgtgaa
    26341 tcatggttgc ggaaccatcg agaatcagcg aggcaacaaa gaagggaatg attgggatta
    26401 aagcgctcca gattaccagc gacatcaccg ccggacgcgt tgagtgcgac atgatctttt
    26461 tattgaagat gttgccacac gcccaactaa atgctgccgc cagggtcaac ataaagccga
    26521 gcatcgccac atgctgaccg ttcagactat cttcgattaa cacaagtacg ccaaaaatcg
    26581 ctaaggcgat ccccgccaat tgtttgccat gcagtcgctc accgaaagta aacgcgccaa
    26641 gcacgatagt aaaaaacgcc tgtgcctgta acaccagcga agccagtcca gcaggcatac
    26701 cgaagttaat ggcacaaaaa agaaaagcaa actgcgcaaa actgatggtt aatccatacc
    26761 ccagcagcaa attcagtggt actttcggtc gtgcgacaaa aaagatagcc ggaaaagcaa
    26821 ccagcataaa gcgcaaaccg gccaacatca gcggtggcat gttatgaagc cccactttga
    26881 tgaccacaaa atttagcccc catacgacca ctaccagtag cgccaacacc ccatcttttc
    26941 gcgacattct accgcctctg aatttcatct tttgtaagca atcaacttag ctgaatttac
    27001 ttttctttaa cagttgattc gttagtcggc gattacgacg gcattaatgc gcaaataagt
    27061 cgctatactt cggatttttg ccatgctatt tctttacatc tctaaaacaa aacataacga
    27121 aacgcactgc cggacagaca aatgaactta tccctacgac gctctaccag cgcccttctt
    27181 gcctcgtcgt tgttattaac catcggacgc ggcgctacgc tgccgtttat gaccatttac
    27241 ttgagtcgcc agtacagcct gagtgtcgat ctaatcggtt atgcgatgac aattgcgctc
    27301 actattggcg tcgtttttag cctcggtttt ggtatcctgg cggataagtt cgacaagaaa
    27361 cgctatatgt tactggcaat taccgccttc gccagcggtt ttattgccat tcctttagtg
    27421 aataacgtga cgctggtggt gctctttttt gcccttatta actgcgccta ttccgttttt
    27481 gctaccgtgt tgaaagcctg gtttgccgac aatctttcct ccaccagcaa aacgaaaatc
    27541 ttctcaatca actacaccat gctcaacatt gggtggacca tcggtccgcc gctcggcacg
    27601 ctgttggtga tgcagagcat caatctgccc ttttggctgg cagctatctg ttccgcgttt
    27661 cccatgcttt tcattcaaat ttgggtaaag cgcagcgaga aaatcatcgc cacggaaaca
    27721 ggcagtgcct ggtcgccgaa agttttatta caagataaag cactgttgtg gtttacctgc
    27781 tctggttttc tggcttcctt tgttagcggc gcatttgcct cgtgcatttc acaatatgtg
    27841 atggtgattg ctgatggtga ttttgccgaa aaggtggttg cggttgttct tccggttaat
    27901 gctgccatgg tagttacgtt gcaatattcc gtgggccgcc gacttaaccc ggctaacatc
    27961 cgcgcgctga tgacagcagg caccctctgt ttcgtcattg ggctggtcgg ttttattttt
    28021 tccggcaaca gcctgctatt gtggggtatg tcagctgcgg tatttactgt cggtgaaatc
    28081 atttatgcgc cgggcgagta tatgttgatt gaccatattg cgccgccagg aatgaaagcc
    28141 agctattttt ccgctcagtc tttaggctgg cttggtgcgg cgattaaccc attagtgagt
    28201 ggcatagtgc ttaccagtct gccgcctttc tcgctgttta tcatcttagc gttggtgatc
    28261 attgctgcgt gggtgcttat gttaaaagga attcgtgcaa gaccgtgggg gcagcccgcg
    28321 ctttgttgat ttaagtcgaa cacaataaaa atttaattca gccttcgttt aggttacctc
    28381 tgctaatatc tttctcattg agatgaaaat taaggtaagc gaggaaacac accacaccat
    28441 aaacggaggc aaataatgct gggtaatatg aatgttttta tggccgtact gggaataatt
    28501 ttattttctg gttttctggc cgcgtatttc agccacaaat gggatgacta atgaacggag
    28561 ataatccctc acctaaccgg ccccttgtta caactgtgta caaggggcct gatttttatg
    28621 acggcgaaaa aaaaccgcca gtaaaccggc ggtgaatgct tgcatggata gatttgtgtt
    28681 ttgcttttac gctaacaggc attttcctgc actgataacg gatcgttgac acagtagcat
    28741 cagttttctc aatgaatgtt aaacggagct taaactcggt taatcacgtt ttgttcgtca
    28801 ataaacatac agcgatttct tccggtttgc ttaccctcat acattgcccg gtccgctctt
    28861 ccaatgacca catccagagg ctcttcagga aatgcgcgac tcacacctgc agtcacggta
    28921 atgttgatat gcccttcaga atgtttgatg gcatggttat cgactaactg gcaaattctg
    28981 atacctgcac gacatgcttc ttcatcatta gccgctttga caataatgat aaattcttcg
    29041 ccaccgtagc gataaaccgt ttcgtaatcg cgcgtccaac tggctaagta agttgccagg
    29101 gtgcgtaata ctacatcgcc gattaaatgc ccgtaggtat cattaaccaa tttaaatcgg
    29161 tcaatatcca acaacattaa ataaagattc agaggctcag cgttgcgtaa ctgatgatca
    29221 aaggattcat caagaacccg acgacccggc aatcccgtca aaacatccat attgctacgg
    29281 atcgtcagca aataaatttt gtaatcggtt aatgccgcag taaaagaaag caacccttcc
    29341 tgaaaggcgt cgaaatgcgc gtcctgccag tgattttcaa caatagccag cattaattcc
    29401 cgaccacagt tatgcatatg ttgatgggca gaatccatta gccgaacgta aggtaattca
    29461 tcgttatcga gtggtcccag atgatcaatc caccgaccaa actggcacag ttcataagaa
    29521 tggttatccg ttatttctgg cttactggca tctctcgcga ccacgctgtg aaacatactt
    29581 accagccact ggtagtgggc atcgatagcc ttattgagat ttaacaagat ggcatcaatt
    29641 tccgttgtct tcttgatcat tgccactcct ttttcacagt tccttgtgcg cgctattcta
    29701 acgagagaaa agcaaaatta cgtcaatatt ttcatagaaa tccgaagtta tgagtcatct
    29761 ctgagataac attgtgattt aaaacaaaat cagcggataa aaaagtgttt aattctgtaa
    29821 attacctctg cattatcgta aataaaagga tgacaaatag cataacccaa taccctaatg
    29881 gcccagtagt tcaggccatc aggctaattt atttttattt ctgcaaatga gtgacccgaa
    29941 cgacggccgg cgcgcttttc ttatccagac tgccgctaat gttgatcatc tggtccggct
    30001 gaacttctcg tccatcaaag actgccgcag gaataacaac attaatttca ccactcttat
    30061 cgcgaaaaac gtaacggtcc tctcctttgt gagaaatcaa attaccgcgt agtgaaaccg
    30121 aagcgccatc gtgcatgttt tttgcgaaat caacggtcat tttttttgca tcatcggttc
    30181 cgcgatagcc atcttctatt gcatgaggcg gcggtggcgc tgcatcctgt tttaatccgc
    30241 cctggtcatc tgccaacgca taaggcatga caagaaaact tgctaataca atggcctgaa
    30301 atttcatact gactccttaa ttgcgtttgg tttgacttat taagtctggt tgctattttt
    30361 ataattgaca aataagaata ctgccaattg ttataaggca tttaaaatca ggcaactagc
    30421 tgt
//
LOCUS       DAPHCV010000059        29828 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.059, whole genome shotgun sequence.
ACCESSION   DAPHCV010000059 DAPHCV010000000
VERSION     DAPHCV010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.059"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(50..1879)
                     /gene="glmS"
                     /locus_tag="RH033_002588"
     CDS             complement(50..1879)
                     /gene="glmS"
                     /locus_tag="RH033_002588"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDV2991758.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2041..3411)
                     /gene="glmU"
                     /locus_tag="RH033_002589"
     CDS             complement(2041..3411)
                     /gene="glmU"
                     /locus_tag="RH033_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDV2991759.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDSTD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3765..4184)
                     /gene="atpC"
                     /locus_tag="RH033_002590"
     CDS             complement(3765..4184)
                     /gene="atpC"
                     /locus_tag="RH033_002590"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDV2991760.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4205..5587)
                     /gene="atpD"
                     /locus_tag="RH033_002591"
     CDS             complement(4205..5587)
                     /gene="atpD"
                     /locus_tag="RH033_002591"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDV2991761.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5614..6477)
                     /gene="atpG"
                     /locus_tag="RH033_002592"
     CDS             complement(5614..6477)
                     /gene="atpG"
                     /locus_tag="RH033_002592"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDV2991762.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6528..8069)
                     /gene="atpA"
                     /locus_tag="RH033_002593"
     CDS             complement(6528..8069)
                     /gene="atpA"
                     /locus_tag="RH033_002593"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDV2991763.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8082..8615)
                     /gene="atpH"
                     /locus_tag="RH033_002594"
     CDS             complement(8082..8615)
                     /gene="atpH"
                     /locus_tag="RH033_002594"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDV2991764.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8630..9100)
                     /gene="atpF"
                     /locus_tag="RH033_002595"
     CDS             complement(8630..9100)
                     /gene="atpF"
                     /locus_tag="RH033_002595"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDV2991765.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9162..9401)
                     /gene="atpE"
                     /locus_tag="RH033_002596"
     CDS             complement(9162..9401)
                     /gene="atpE"
                     /locus_tag="RH033_002596"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HDV2991766.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9448..10263)
                     /gene="atpB"
                     /locus_tag="RH033_002597"
     CDS             complement(9448..10263)
                     /gene="atpB"
                     /locus_tag="RH033_002597"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDV2991767.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPHNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10272..10652)
                     /gene="atpI"
                     /locus_tag="RH033_002598"
     CDS             complement(10272..10652)
                     /gene="atpI"
                     /locus_tag="RH033_002598"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HDV2991768.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11269..11892)
                     /gene="rsmG"
                     /locus_tag="RH033_002599"
     CDS             complement(11269..11892)
                     /gene="rsmG"
                     /locus_tag="RH033_002599"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDV2991769.1"
                     /translation="MLNKLTSLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLHVPALDGERHLVVIKANKI"
     gene            complement(11956..13845)
                     /gene="mnmG"
                     /locus_tag="RH033_002600"
     CDS             complement(11956..13845)
                     /gene="mnmG"
                     /locus_tag="RH033_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDV2991770.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14224..14667)
                     /gene="mioC"
                     /locus_tag="RH033_002601"
     CDS             complement(14224..14667)
                     /gene="mioC"
                     /locus_tag="RH033_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HDV2991771.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14757..15215)
                     /gene="asnC"
                     /locus_tag="RH033_002602"
     CDS             complement(14757..15215)
                     /gene="asnC"
                     /locus_tag="RH033_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HDV2991772.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15367..16359
                     /gene="asnA"
                     /locus_tag="RH033_002603"
     CDS             15367..16359
                     /gene="asnA"
                     /locus_tag="RH033_002603"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDV2991773.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLEL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(16364..17815)
                     /gene="viaA"
                     /locus_tag="RH033_002604"
     CDS             complement(16364..17815)
                     /gene="viaA"
                     /locus_tag="RH033_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HDV2991774.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(17809..19305)
                     /gene="ravA"
                     /locus_tag="RH033_002605"
     CDS             complement(17809..19305)
                     /gene="ravA"
                     /locus_tag="RH033_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HDV2991775.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERNALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDISLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            19528..21396
                     /gene="kup"
                     /locus_tag="RH033_002606"
     CDS             19528..21396
                     /gene="kup"
                     /locus_tag="RH033_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HDV2991776.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVVLILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            21563..21982
                     /gene="rbsD"
                     /locus_tag="RH033_002607"
     CDS             21563..21982
                     /gene="rbsD"
                     /locus_tag="RH033_002607"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDV2991777.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPHLHETLLTHLEQLQQHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            21990..23495
                     /gene="rbsA"
                     /locus_tag="RH033_002608"
     CDS             21990..23495
                     /gene="rbsA"
                     /locus_tag="RH033_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HDV2991778.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPNSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            23500..24465
                     /gene="rbsC"
                     /locus_tag="RH033_002609"
     CDS             23500..24465
                     /gene="rbsC"
                     /locus_tag="RH033_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDV2991779.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            24490..25380
                     /gene="rbsB"
                     /locus_tag="RH033_002610"
     CDS             24490..25380
                     /gene="rbsB"
                     /locus_tag="RH033_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HDV2991780.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            25506..26435
                     /gene="rbsK"
                     /locus_tag="RH033_002611"
     CDS             25506..26435
                     /gene="rbsK"
                     /locus_tag="RH033_002611"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDV2991781.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDISPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            26439..27431
                     /gene="rbsR"
                     /locus_tag="RH033_002612"
     CDS             26439..27431
                     /gene="rbsR"
                     /locus_tag="RH033_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HDV2991782.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(27397..28824)
                     /gene="mdtD"
                     /locus_tag="RH033_002613"
     CDS             complement(27397..28824)
                     /gene="mdtD"
                     /locus_tag="RH033_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HDV2991783.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFYLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(28847..29539)
                     /locus_tag="RH033_002614"
     CDS             complement(28847..29539)
                     /locus_tag="RH033_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDV2991784.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAAMVGTAEQKAHLNTLMAEMVALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         7188 a         7643 c         7626 g         7371 t
ORIGIN      
        1 tatgaaaaag ggccgccggt cggcagccct tttttatcaa cgatggagat tactcaaccg
       61 taaccgattt tgccaggtta cgcggctggt caacgtcggt gcctttgatc agcgcgacgt
      121 ggtaagccag cagctgcagc ggaacggtgt agaagatcgg tgcaatcacc tcttccacat
      181 gcggcatctc gatgatgtgc atgttatcgc tacttacaaa acccgcatcc tgatcggcga
      241 agacatacaa ctgaccacca cgcgcgcgaa cttcttcaat gttagatttc agtttttcca
      301 acaactcgtt gttcggcgca acgacgataa ccggcatatc ggcatctatc agcgccagcg
      361 gaccgtgttt cagttcgccc gcagcgtagg cttcagcgtg aatgtaagag atctctttca
      421 gcttcaatgc accttccagc gcgattgggt actgatcgcc acgtcccagg aacagcgcgt
      481 gatgtttgtc agagaaatct tctgccagcg cttcaatgcg tttgtcctga gacagcatct
      541 gctcaatacg gctcggcagc gcctgcagac catgcacgat gtcatgttca atggaggcat
      601 ccagaccttt caggcgagac agtttcgcca ccagcatcaa cagcacagtt aactgggtgg
      661 tgaatgcttt ggtggaggcc acgccaattt ccgtaccggc gttggtcatc agcgccagat
      721 cggattcgcg caccagagaa gaacccggaa cgttacagat tgccagcgaa ccaaggtaac
      781 ccagctcttt cgacagacgc aggcccgcca gggtatccgc ggtttcgcca gactgtgaca
      841 aggtgatcat caggctgtta cggcgcacgg cagatttgcg atagcggaat tcagaggcga
      901 tttcgacgtc gcacggaata cctgccagcg attcaaacca gtagcgggaa accataccgg
      961 agttataaga agtaccacag gcgaggatct gaatatgctc aaccttcgac agcagttcgt
     1021 cggcgttcgg tcccagctcg cttaaatcaa cctgaccgtg gctgatgcgc ccggtaaggg
     1081 tgtttttgat cgcgtttggc tgttcataga tctctttctg catgtagtga cggtaaatac
     1141 ctttatcgcc cgcgtcatat tgcagattgg attcaatatc ctgacgtttt acttccgcgc
     1201 cagttttatc gaaaatgttt accgaacggc gagtgatttc cgcaatatcg ccctcttcaa
     1261 ggaagataaa gcgacgagtc actggcaaca gcgccagctg gtcagaagcg ataaagtttt
     1321 cgcccatgcc cagaccaatc accagcgggc taccagaacg tgccgccagc agggtatccg
     1381 ggtgacggga gtccatgatc actgtaccgt acgcaccacg cagttgcgga atagcacgca
     1441 gaacagcctc acgcagagta ccgccttgtt tcagctccca gttcaccaaa tgtgcaatca
     1501 cttcggtgtc ggtttcagaa acgaaggtat agccacgcgc ttttaactct tcacgcagcg
     1561 gttcatggtt ttcgatgatg ccgttatgca ccaccacaat gtgttcagaa acatgcggat
     1621 gcgcgttagc ttctgaaggt tcaccgtggg tcgcccagcg agtatgagca ataccggtgc
     1681 cgccatgcag aggatgttct tccgctgcct gagccagcat ctgtacttta ccgaggcgac
     1741 gcaggcgggt catatgacct tctgcatcaa caacggccag accggcagag tcatatccgc
     1801 ggtattccag acgacgtaaa ccttcaagaa ggatttctgc tacatcacgt tgcgcgatcg
     1861 cgccaacaat tccacacata gtttttgatt ccgatttata tcgttgtcgg tcaacctgta
     1921 tgcccgtttt ttcgggcgcc ccgagccttg tagagagtgg ggttattttt atagttactg
     1981 cttgtgggcg ggagattatg ttatctcctc atcccatatg accggatttc ccagccagaa
     2041 ttactttttc tttaccggac gacgccagcc ttctttttga gtctgcggca cacggctgat
     2101 cgccagggcg ttttcgccga cattacgcgt cacagttgta cccgcagcaa tggtcgcgcc
     2161 tttgcctact gttaccgggg ccaccagctg agtgtcggaa ccgacaaaca catcgtcacc
     2221 gataatggtc ttaaatttat tcgcaccatc gtagttgcag gtaatggttc ccgcgccgat
     2281 gttaacgtta tcgccaattt ccgcatcgcc caggtaagtc agatgaccag ctttcgagcc
     2341 tttacccaga cgcgcttttt tcatctcaac gaagttaccg acgtgtgcac cttccagcaa
     2401 ctcagcacca ggacgcagac gggcaaacgg gccaatggta caggccgctg ccagattcgc
     2461 atcttccaca acggtatacg gactgatttc gcaatcatcg ccaatcacgc tgtttttaat
     2521 cacgcaaccg gtgccgattt tcacgcgatg accgagagtc acgttgccct cgatgataac
     2581 gttagtatca atttcaacat cgcgcccgtg agtaagcgta ccgcgcagat caaaacgcgc
     2641 cggatcgcgc agcataacgc ctgctaacag cagtttttca gcctgttcgg actggtaaac
     2701 gcgctccagt cgggagagtt gcaggcggtt attcacgcct tctacttcgc ttaaacgttg
     2761 cggatgaacg gcgacgattt cacgcccttc ctgatacgcc agcgcaataa tgtcggtgat
     2821 gtagtattca ccctgagcgt tattgttggt cagcttcgcc agccagcgtt tcatatctgc
     2881 gccgttggca atcagaatgc cggtgttgat ctcctgaatc tgacgctgct cgtcggtgga
     2941 gtctttgtgc tcaacaatgc cggtaacttt gccgttttca cgggtgatac gtccataacc
     3001 ggtcggatca tccagtttta ccgtcagcag accaatgcca ccctgcggtt tagcatcacg
     3061 caggcgctgg agtgtttcga cagagatcag cggcacgtcg ccgtagagca ttaaaatgtc
     3121 ttcatcatcg gcaaagaaag gtgcggcctg ctgcatcgca tgacctgtac ccagctgttc
     3181 tgcctgaagc acccagttca ggttgtcatc tttcagcgcc tgtttaagca gatcgccgcc
     3241 gtgaccgtaa accaggtgaa cgtgcgctgc gcctaattca ttcgcagcat caatgacatg
     3301 ctgaaccatc gctttcccgg caagggtatg cagcactttc ggaagatcgg aatacatgcg
     3361 cgtgcctttg cctgcggcaa ggatcactac gctcatagca ttattcaaca tacgcgtcct
     3421 gactgtaatt tgagaacgaa tttaaaccgc ttcaccttga aaaaactaca tttttttcat
     3481 cgtgaaatgg acagaggata aattgttcaa taacggatta tccccgctga cgaaagcgcc
     3541 attttcgacc atcgtcacgc cttttgtctg cagaaaatta aggcagtacc ttcatttaag
     3601 cgtcaggtgg atgtttttgc tcttatttcg atcaatgaat aaacgggaaa acaggggtgt
     3661 tttatatcat caggatctat gtgaacgctt ttcaggacgg gtcaatacgc gtaaaaaaaa
     3721 gccagcctgt tgccagactg gcttttgtgc ttttcaagcc ggtgttacat cgcttttttg
     3781 gtcaactcga taacgcgcag ctgcgcgatc gctttggcca gttccgcaga cgcctgagcg
     3841 taatctacgt cgccgtgaga gctgctaatg tgctcttcag ccttacgttt cgcttccatg
     3901 gctcgcgctt cgtcgagatc ctggccgcga attgcggtgt cggccagaac agtcacgttg
     3961 ccaggctgca cttcaagaat gccgccagac agatagataa actcttcgtg accgtgctgt
     4021 ttcacgatgc gaatcatacc aggcttaatg gcggtgagca gcggtgcgtg gccagggtag
     4081 atccccagtt caccttcgct acccgttacc tggattttct cgaccagacc agagaacatt
     4141 tgttgctctg cgctgacgac gtccaggtgg taagtcattg ccatatcacc ctccgattaa
     4201 ggcgttaaag ttttttggct ttttccacag cttcttcgat ggaaccgacc atgtagaacg
     4261 cctgctccgg caggtgatcg tattcgcctt ccatgatgcc tttaaagcca cggatggtgt
     4321 ctttcaggga gacgtattta cccggagaac cggtgaatac ttctgccacg aagaacggct
     4381 gggacaggaa gcgctggatc ttacgagcac gcgctaccac cagtttgtct tcttcagaca
     4441 gttcatccat acccaggatg gcgataatgt ctttcagttc ctgataacgt tgcaggatgg
     4501 actgaacgcc acgcgcggtg tcgtagtgtt cctgaccaac caccagcggg tccagctgac
     4561 ggctggtgga gtccagcggg tcaacggccg ggtagatacc cagagacgcg atctgacggc
     4621 tcagtaccac ggttgcgtca aggtgcgcaa aggtggttgc cggagacggg tcagtcaagt
     4681 catccgcagg tacgtatact gcctgtacgg aggtgataga accggttttg gtggaggtga
     4741 tacgttcctg cagaacgccc atctcttccg ccagggtcgg ctgataacct accgctgaag
     4801 gcatacggcc cagcagtgcg gatacttccg taccggccag ggtgtaacga tagatgttgt
     4861 caacgaacag cagaacgtca cgaccttcgt cacggaattt ctcagccatg gtcagaccgg
     4921 tcagagcaac gcgcagacgg tttcccggcg gctcgttcat ctggccatac accagggata
     4981 ctttgtcgat aacgttggag tcggtcattt cgtggtagaa gtcgttaccc tcacgagtac
     5041 gttcacctac gcccgcaaac acagagtaac cggagtgctc gatcgcgatg ttacgaataa
     5101 gctccatcat gtttacggtt ttacctacac ccgcaccacc gaacagacca actttaccgc
     5161 ccttagcgaa cggacacatc aggtcgataa ctttgatacc ggtttccagc agttcctgag
     5221 agtttgacag ctcttcgtag gaaggtgctg cgcggtgaat cgcccaacgc tcttcttcac
     5281 cgatctcgcc tttcatgtcg accggttcac ccagtacgtt catgatacgg cccagagtcg
     5341 ctttacctac cgggacttcg atcgggtgtt cgaggtcttt tacatccaga ccgcgacgca
     5401 gaccgtcgga ggaacccatt gcgatggtac gcacgatacc gccgccgagc tgctgctgaa
     5461 cttccagcac cagacgctca ttaccatttt gcacctcaag agcatcgtac acgcgcggta
     5521 cggcatcctg agggaattcg acgtcaacta cggcgccgat tacctggaca atctttccag
     5581 tagccatctt aaatcctcta cgaaataacc tgtttaaacc gcggcggccc ccgagacgat
     5641 ctcggtgagt tcctgagtaa tgctggcctg acgagctttg ttgtatacca actgcagctc
     5701 tttaatcagg ctgccgccat tgtcggtcgc ggctttcatc gccaccatac gggcggcctg
     5761 ctcgctggcc aggttttcaa ccacgccctg ataaacctga gattcgacat aacgacgcag
     5821 cagggtatcc agcaacgcct tcggatcggg ttcgtacagg taatcccagg atttatgttt
     5881 cagatcatca tcatctgatg ccggtaacgg cagcagctgg ctgatggtcg gaacctgaga
     5941 catggtgtta ataaatttgt tgctgacaat gtaaagcttg tccagacggc cttcgtcgta
     6001 ggcctgcaac atcactttta ccggaccgat cagttcggac agggaagggt tatcccccat
     6061 gccggtgacc tgggcaacaa cattgccgcc cacggagttg aagaacgaca cgcctttcga
     6121 gccgatcatt gcgaggtcgc attgaacgcc tttgtcggtc caggtcttca tttccgccag
     6181 cagttttttg aacaggttaa tgttcaaacc accgcacaaa ccacggtcgg tcgacaccac
     6241 caggtagccc acgcgtttaa cgtcgcggtc ttccaggtaa gggtgcttat attccagatt
     6301 accgtgtgca aggtgaccaa tcactttgcg catggtttct gcataaggac ggctggccgc
     6361 catgcgatcc tgcgatttac gcattttgga agcggcgacc atctccatcg ctttagtgat
     6421 cttttgcgtg ttctggacgc ttgcgatctt actacgtatc tcttttgcgc cggccatgag
     6481 cttctcctca atgccttgcg gcctgcccta aggcaagccg ccagacgtta ccaggattgg
     6541 gttgctttga aggaatcgag gatgcctttc agcttgcctt cgatttcgtc gttgtagcca
     6601 ccggtctggt tgatctcttg catcaacgga gcgtgatcac ggtcgacgta agccagcaga
     6661 gcggcttcga agctgccgat tttcgacagt tctacatccg ccaggtaacc acgttctgct
     6721 gcgaacagaa ccagagactg ttgcgcaaca gacatcggcg catactgttt ctgcttcagc
     6781 agttcggtca ctttctgacc gtggtcaagc tgcttacgtg ttgcatcgtc aaggtcggat
     6841 gcaaactgag agaacgctgc cagttcacga tactgtgcca gagcggtacg gataccaccg
     6901 gacagttttt tcatgatctt ggtctgtgct gcaccaccaa cacgggatac ggaaataccc
     6961 gggttaaccg caggacgaat accggcgttg aacaggttgg tttccaggaa gatctgacca
     7021 tcggtaatgg agattacgtt ggttggaacg aacgcagaaa cgtcacccgc ctgagtttcg
     7081 ataatcggca gcgcagtcag agaaccggtt ttccctttca cttcaccttt ggtgaaggct
     7141 tcaacgtatt cggcgttaac acgcgcagca cgctccagca gacgagagtg gaggtagaat
     7201 acgtcgcccg ggaatgcttc acgtcctggc ggacgacgga gcagcaggga gatctgacgg
     7261 taagcaacag cctgtttaga caggtcatcg taaatgatca gcgcatcttc accgcggtca
     7321 cggaagtatt cgcccattgc acaaccagcg tacggtgcca ggtattgcag tgcagcggat
     7381 tcagacgcgg ttgctaccac aacgatggtg ttagccagtg cgccgtgctc ttccagttta
     7441 cgtaccacgt tagaaatggt ggacgctttt tggccgatag cgacatagat acatttgata
     7501 ccggaatcgc gctggttgat gatggcatcg atagccagtg cggttttacc ggtctgacgg
     7561 tcaccgatga tcaattcacg ctgaccacga ccgattggga tcatggagtc aacggcttta
     7621 taaccggtct gtaccggctg atctacggac tgacgttcga taacgcccgg agcgattgct
     7681 tctacagcag agaagccgtc gtgatccagc ggacctttac cgtcgattgg tgcacccaga
     7741 gtgttaacca cacggcccag caggccacgg ccaaccggaa cttccaggat acggccagta
     7801 cacttaactt tcatgccttc ggcaaggtca gcgtacggac ccataacaac cgcacctaca
     7861 gagtcgcgct cgaggttcag tgcgatagcg taacggttac ccggcaggga gatcatttca
     7921 ccctgcatac aatcggccag gccgtgaatg cggataacac cgtcacttac agaaacaata
     7981 gtaccttcgt tgtgagcttc actcacaaca ttgaactgag caatgcgctg cttgatcagt
     8041 tcgctgattt cggtggaatt cagttgcatg ctccagtccc cttaagactg caagacgtct
     8101 gcaaggcgct caagacgacc gcgtacgctg ccatcaatga ccatatcacc cgctcggatg
     8161 ataacgcctg ccattacaga cttatcgatt ttgcaattca gcttaacttt gcgtgacaga
     8221 cgtttttcca tcgcagcaga aattttcgcg agctgttgtt cactcagtgc ggcagcggaa
     8281 atgacgtcta cctcagcggt agcctcactc acggcacgca ggtgaataaa ctgctccaga
     8341 acatccggga gcgcgttaag acgaccattt tcagccataa cccgaatcag gttctgaccg
     8401 ttttcgtcca gttgctcacc acaaactgcg ataaacgact cggcgagcgt ttctggcgca
     8461 agcgcgccag agagaagctc tgccatttgt tcgtttttgg ttacctcggc ggcaaacgcc
     8521 agcatgtcct gccagcgttc tacactttgg tgttcgacgg caaagtcaaa agctgctttg
     8581 gcgtaggggc gagctaccgt aataaattca gacatcagcc cctccctcct tacagttcag
     8641 cgacaagttt atccacgatg tcgctgttag cagcttcatc cacggaacgt tcgatgatct
     8701 tctcggcgcc agcaacagcc aggatagcaa cttgcttacg cagctcttca cgggcacgtt
     8761 tacgctcggc ttcaatttcc gcctgcgcct gggccacgat tttagtacgt tcctgttctg
     8821 cctcagcttt cgcttcgtcc agaatctgcg agcggcgttt gttagcctgc tcgatgatta
     8881 cctgggcttc cgctttcgct tttttcagct ggtcggtcgc gctggccttt gcaaggtcaa
     8941 ggtccttatg tgctcgttct gcggaagcaa ggccgtcagc aatttctttt tgacgttttt
     9001 cgatggctgc cattaatggc ggccatacgt acttcatgca gaacagaacg aacaggacaa
     9061 acgcgatggc ctggccgagg attgttgcgt taagattcac agcacaatgc ctctatttag
     9121 ttaacgttct gatattgctc tttaaataaa agcaacgctt actacgcgac agcgaacatc
     9181 acgtacagac ccagacctac agcgatcatc gggatagcat ccaccagacc cataacgata
     9241 aagaactgag tacgcagcag aggaatcaga tcaggttgac gcgctgcgcc ttccaggaat
     9301 ttacccccga ggatgccgat accgatcgca gcaccgattg ccgccagacc catcatcaca
     9361 gcggcagcca tgtacagcag atccatattc aggttttcca tgacagtctc cagtttgttt
     9421 cagttaaaac gtagtagtgt tggtaaatta atgttcttca gacgccatcg acagatagac
     9481 gatcgtcaga accatgaaga tgaaggcttg cagcgtaatg atcaggatgt ggaaaatggc
     9541 ccacggcaca ttcaggatcc actgtgacca ccacggcaac agaccagcaa tcagaatgaa
     9601 aatcagctca ccggcataca tgttaccgaa cagtcgcaaa ccgagtgaaa ctggtttgga
     9661 cagcaggctt accccttcaa ggattaagtt gacaggaatg aacgcccagt gattgaacgg
     9721 ctgcagcgtc aactctttcg tgaagccgcc gatgcctttc attttgatgc tgtagaacag
     9781 aatcaggata aatacgccca gtgccataga cagcgttacg ttcacgtcag cagacggaac
     9841 cacacgcagt gcaggcagac ccagtacatg ttcagcaatg tacggcagca ggtcgatagg
     9901 cagtaaatcc atcaggttca tcaggaatac ccagacgaag atcgtcaggg ccagcggagc
     9961 aatcagcttg cttttgccat ggtacatgtc tttcacgcta ccattaacaa agccgatcac
    10021 cagctcaatc gcggtctgaa acttacctgg cacgccgctg gttgcctttt tggctacact
    10081 acggaataaa accaggaaca acagacccag caccaccgag aagaacatgg agtcaatatt
    10141 gattgtccag aaggtggctg gggggttatg tggatccacc agcgagaatg tacgcaggtc
    10201 cagctgaagg ttattcaggt ggtgtcctat gtaatcctgc ggcgtcatat tttctgaagc
    10261 catgatgcct tttacccttt gttgttaatt acagccggtg ccagtatctg aaccaccagc
    10321 accaaaaccc acgtaacgat cagcggcaag aataccgcct ttaaaaccgc caacgccacc
    10381 accagtaaca ccaacatcgc cagaactttg aaagcttcgc caaatgcgaa tgtccaggcc
    10441 acccggcctt tcgctggtgt atgcgcctgg tgacgccagg caaatatcat aaacaaaacg
    10501 ttaggcagaa agactgccag gcccccgctt attgcagaga cgccccagaa ggggtctttg
    10561 aggctgaaca gcaatccact tgctatcacc accagtaact gaacgagcag aagcttccga
    10621 gcaacgtttc gactcacgag cgacacagac atcacgtttt tcactcctgc tcccttcgag
    10681 gtatgccgcg tgtcgtataa aactttcttt aaggcttaga gtcaagcatc aaaaagcggt
    10741 caaattatac ggtgcgcccc cgtgatttca aacaataagt agccaaaagg tgaataaatg
    10801 tttaaatatt tttccagtgc atacaattgc gacttttctg caaaccatgt tcgatcatga
    10861 aaaactgtaa ataacgcgta aacactggtg ataaagcgtg cttcagatca catattgagc
    10921 atgttcgcgc acagcatatt tatttacttg gcaaatgatg cctttgcaag tttatgatat
    10981 ttcagtctaa aaacagatac tgttttaata aatgacattt acacaacaaa aaccacccat
    11041 tgacattttt aataatgttt taacagccaa tgatgattct tagcgccgat ttttagcaga
    11101 ctgatatttt cacttatgac ttattttctg cttaccaaaa aaagccacgt tatcttgttg
    11161 atgcaaaaga gtgaacgtgg cgttaaatgt aaccagttat atcagtagaa aacctggttg
    11221 ttgttaacag tctaaccggt caatttttta tgattttttt gataaaaatt aaattttatt
    11281 tgctttaatc accaccagat gacgttcgcc atccagggcc ggaacgtgaa gtttaaccac
    11341 tgattcgacc tgatattctt cgggcaacaa agcgatttca tcttccggca tttggccttt
    11401 cagcgcgtag aaacggcctt gctcaccagg aagatggtga caccagctca ccatatcgtt
    11461 cagagaggca aaagcgcggc taattacgcc atcaaatggc ggctctgaag gaaactcttc
    11521 taccctgctc tgtactggct caatattctc cagtttaagc tcatgttgca cctgacgaag
    11581 gaaacgcacg cgtttaccaa ggctatccaa cagagtgaaa tgggcttcag gacgcacgat
    11641 agagagtgga atgccaggca gtcctggacc ggtgccgaca tcgataaacc gttcaccttg
    11701 cagatacggt gccaccacaa tgctatcgag aatatggcgt accagcatct cattaggatc
    11761 gcggaccgaa gtcaggttgt acgctttatt ccatttatgc agcatattca cataggcaat
    11821 aagctggttt ttctggtgat cggtaagcga aatacctgcg tctttcagca gagaggtgag
    11881 tttgttgagc acggtgatta cctgttcttg atgcgttgcc cggtaagcgg gtgcttacca
    11941 ggcattttta atgcgttatg cgctacgacg cagcataccc tgttttttca gccacaccag
    12001 cagaatggag atagccgcag gcgtgacgcc agaaatacgc gaagcctggc caatagaggc
    12061 cggtttgtga tcgttaagtt tggcgatcac ttcgttagaa agaccggata cctggcggta
    12121 atccagcgtc gctggtagca gggtattctc gttacgcagc tgcttttcga tctcatcttg
    12181 ctggcgcgcg atataacctt cgtatttaac ctgaatctca acctgttccg cagcctgttc
    12241 atctgtcaac gcaggggcaa acggtgtcag cgtggttaat ttttcataag tcatttcagg
    12301 acgacgtagc agatcttcac cactggcttc acgggaaagc ggcgcagtca ggtgagcatt
    12361 cacttcggct gcagcttccg ccgacggggt tacccaggtc gatttcagac gctgacgctc
    12421 acgctcgata ttctcaagtt tctcgttaaa gcgcgcccaa cgttcgtcat ccaccaggcc
    12481 cagttcacga ccgatttcag tcaaacgcag atctgcatta tcttcgcgca gcatcagacg
    12541 atattctgcg cgcgaggtaa acatacgata cggttctttg gttcctaaag tgcacaggtc
    12601 atcaaccagt acgccgagat acgcctgaga acgcgctgga gcccaacctt ctttgtctgc
    12661 agacagacgg gcagcgttaa gaccagccag caaaccttgc gcagcggctt cttcgtaacc
    12721 ggtagtgccg ttaatctgac cagcaaagaa cagcccctgg ataaacttgc tctccagcgt
    12781 cggtttcagg tcgcgtggat cgaagaagtc atactcaatg gcataacctg gacgcacgat
    12841 cttcgcgttt tccatcccct gcatggagcg gacgatttgc atctgcacat cgaacggcag
    12901 gctggtggag ataccgttcg gataaatttc gtttgaggtc agcccttccg gttcaaggaa
    12961 gatctgatgc tgatttctgt cggcgaagcg catgactttg tcttcgatcg acgggcagta
    13021 gcgtgggccg acaccttcga tcacccctgc gtacattggg ctacgatcga ggttactgcg
    13081 gatcacatca tgggttttct cgttggtatg agtgatataa cacggcacct gctgtggatg
    13141 ctgggacgca ttacccataa acgagaatac cggcattggg ttatcgccat gctgttgcgc
    13201 cagcacgcta aagtctatgg ttcgagcatc aatacgcggc ggagtcccgg ttttcagacg
    13261 accaacgcgc agcggcagtt cacgcaaacg gcgagaaagc ggaatggacg gcggatcacc
    13321 agcacggcca ccgctgtagt tatccagacc gatatgaatt ttaccgtcca ggaacgtccc
    13381 aacggtgagc acgacggctt tggcacggaa cttcagtccc atttgggtaa cagcaccgac
    13441 cacgcgatcg ttttcgacaa taagatcttc aaccgcctgc tggaagatca tcaggttcgg
    13501 ttggttctcc agcgccgtac gtaccgcctg acggtagagc acacgatccg cctgagctcg
    13561 ggtagcgcga accgccggtc ccttgcttgc gtttagtatc ctaaactgga tacccgcctg
    13621 atcgatcgct ttcgccatca gaccgccgag tgcatccact tcttttacca gatgtccctt
    13681 cccaataccg ccgatcgccg ggttgcagct catctgcccc agagtgtcga tattgtgtgt
    13741 caaaagcaga gtctgttgac ccatacgcgc cgcggccatc gcggcctcgg tgcctgcatg
    13801 acccccgcca atgatgatga cgtcaaaagg atccggataa aacatggtga ttgcctcgca
    13861 taacgcggta tgaaaatgga ttgaagcccg ggccgtggat tctactcaac tttgtcggct
    13921 tgagaaagac ctgggatcct gggtattaaa aagaagatct atttatttag agatctgttc
    13981 tattgtgatc tcttattagg atcgcactgc cctgtggata acaaggatcc ggcttttaag
    14041 atcaacaacc tggaaaggat cattaactgt gaatgatcgg tgatcctgga ccgtataagc
    14101 tgggatcaga atgaggggtt atacacaact caaaaactga acaacggttg ttctttggat
    14161 aactaccggt tgatccaagc ttcctgacag agttatccac agtagatcgc acgatctgta
    14221 tacttatttg agtaaattaa tccacgatcc cagccattct tctgccggat cttccggaat
    14281 gtcgtgatca agaatgttga tcttcagtgt ttcgcctgtc tgttttgcac cggaattttt
    14341 gagttctgcc tcgagtttat cgatagcccc acaaaaggtg tcatattcac gactaccaat
    14401 accgattgcg ccaaagcgga ccgcagaaag atcgggcttc tgttcctgca atgcttcata
    14461 gaaaggagaa aggttgtccg gaatatctcc ggcaccgtgg gttgagctga taaccagcca
    14521 gatcccagag gcagataaat cttccaacag cggaccgtgc agcgtttcgg tggtaaaacc
    14581 cgcctcttcc agcttttcag ccaggtgttc tgctacatat tcggcaccgc cgagggtgct
    14641 gccgctgata agagtgatat ctgccataaa ccgccacctt tattaagagt ggcatattgt
    14701 acgctgtgaa cgcgttggga tctacctgtg gaaaagtatg ggattaaaaa agccgatcag
    14761 ggcttgatgg tacgcatgat cgggttttgc aggacgatca atgtctcggt ggactgaatt
    14821 tcatcaattg tttggatctt gttgataagt acatgctgga gagcgtcgat cgaacggcac
    14881 atcactttta taaagatgct gtagtggccg gtggtgtaat aggcttcagt gacttcatca
    14941 aggctttcca gtttcgccag cgcggaagga tagtctttgg cgctctttaa tataatgccg
    15001 ataaagcagc ctacgtcata accgagctgc ttcgggctga catcaatacg cgccccggta
    15061 atgatccccg cctgcttcat tttctctact cgaacgtgaa tcgtccccgg actgacgcca
    15121 aattgtttcg ccagttcggc gtaagcggtg cgcgcattgc ccattaatgc ttccaggatg
    15181 ccacggtcca gattgtcgat tagataattt tccataggat tttcttatgc ggattgatga
    15241 ttcattctat tttagccttc ttttttaatg aatcaaaagt gagttaggct ttttattgaa
    15301 tgattattgc atgcgtgtcg gtttttgttg cttaatcata agcaacagga cgcaggagta
    15361 taaaaaatga aaaccgctta cattgccaaa caacgtcaaa ttagcttcgt gaaatctcac
    15421 ttttcccgtc aactggaaga acgtctgggg ctgatcgaag tccaggcgcc gattcttagc
    15481 cgtgtggggg atggcacgca ggataacttg tcgggctgtg aaaaagcggt gcaggtaaaa
    15541 gtgaaagctc tgcctgatgc ccagttcgaa gtggttcatt cactggcgaa gtggaaacgt
    15601 cagacattag ggcaacacga cttcagcgcg ggcgaagggc tgtacacgca catgaaagcc
    15661 cttcgccccg atgaagaccg tctttctccg ttgcactcgg tctatgttga ccagtgggac
    15721 tgggaacgcg taatgggcga cggtgagcgt caattctcga ctctgaaaag cacggtagag
    15781 gcgatctggg cgggaattaa agcaaccgaa gctgcggtta gcgaagagtt tggcctggca
    15841 ccgttcctgc cggatcagat ccacttcgta cacagccagg agttactgtc tcgttatccg
    15901 gatctcgatg ccaaagggcg tgagcgggcg attgcgaaag atcttggcgc ggtattcctc
    15961 gtcgggattg gcggcaagtt gagtgatggt catcgtcacg acgtgcgcgc accggattat
    16021 gatgactgga gcaccccgtc agagctgggc catgcgggtc tgaacggcga tattctggta
    16081 tggaacccgg tactggaaga tgcgtttgag ctttcttcca tggggatccg cgtggatgcc
    16141 gatacgttga aacatcagct ggcgctgacc ggtgatgaag atcgcctgga gctggagtgg
    16201 catcaggcgc tgctgcgcgg tgaaatgccg cagaccatcg gcggcggtat cgggcagtct
    16261 cgtttgacca tgctgctgct gcaactgcca catatcggcc aggttcagtg tggtgtatgg
    16321 ccggcagctg ttcgcgagag cgtcccttct ctgctgtaat aatttatcgc cgccagcgtc
    16381 tgagcaggcg gcttcgcatc ccggtatcaa agcgccagat atgatcgaaa atgcgcatga
    16441 tgccgggttt gccatgtgca gacatcgcca cggcatgaaa gcgatgctga tgtacgcgct
    16501 gtagctcttt cactttactc gtcacgtcgt caggcaaccg ctgggcgata aaatcagaaa
    16561 tcaccaccgc atcggcatca aaccattccc tgctttgcaa gcgttccata atggcgcgaa
    16621 aacaactggc aagatcggtg ccgccacgaa actgctggct taaaaaacgg attgcttgtt
    16681 cgatgccttg tgggcctgaa agctcataac ggacgatctc ggtggaaaat aacataatat
    16741 agcagcgccg gttttcagcg agagcaatgc gcatcaaggc caggcagaac gctttcgcac
    16801 actgttcatt aaagccgccc attgagccgg aagtatccac acagacaata aacggtccgc
    16861 gcggctgttc gtcgtaatct ttatgtacca ccgggcgttc gatcactttt tcacgccacg
    16921 actcaccgtg caggcgatag gtgagcaact gtttttccac cagccgacgg taaaactcat
    16981 actccagttc cgttatcccc agtgtcgcca gttctggcgg caggagacgt aaaatatcat
    17041 cgctttgttg cagaccatca acctgctcag gaaccgtcgc cggttcgcgc accatggtgc
    17101 ggaaggtttc catctgcgca tcgttgcgcg gtattgattt ggcttcccgt gaacgcccca
    17161 actgttctgc caggcgtttc agttccggct gttcgttaag aaattcaccg tatttcacaa
    17221 tcaactgata gtcgccgcgt ttaagctgac cggcgctcat atcccacaga cgaccagctg
    17281 cggtattgtt atctgcgaga atcggttcaa gttgcccgct cagcgtcatg cgttcctgaa
    17341 cttcactcaa cagttgttcg cgttcttctt ctaatagctg ctgatttagc gtcgttgctt
    17401 gcacgatcag acttaaacgc cagcgctgga gaaaaagcgt gtgtaacgct gaagtgatcg
    17461 tactgttagc atcaaccaac tgtcgggctt gttctgccca tggagaattc agacgatgca
    17521 gtaagtccag gatctgtggt agctgcacaa taaactgcgg cgtggagagg agctggcttt
    17581 gctgatagca catcacctct tcggtgagtt ccggcgggac tcgggcatct ttcagccgac
    17641 tgcgcagcgc ctcacgccag cggggaacat catcagtgat tgccgccttc agtcgtggga
    17701 atttttcaaa gaagaccgcc agctgcggtg aggccagcag cgcgatgatc atctcttcga
    17761 tcaatccctc ttcgctgacg gccagcatca cattaagcgt atccagcgtt agcattgttg
    17821 tgcctggcga atctgtgcac cgacatcctg taggctggct tcaatgcgac ctaaccagtc
    17881 gccagggata aacagacatt tttgctgttc gctgaataaa gcgtgttgct tgcgccagtc
    17941 gctttcaagc tcttccagtt gttgttttat ttcacctggt agaccttcag ccgatgaacc
    18001 gggaagtgcc agcgtactgc cttgtaaact tatatcgcgt acaacaagat gttgggcact
    18061 atcaacttcc agattcagtt tctgggcaaa gccaataccg ttcagtttgc cgcgaatttc
    18121 accaccttta ctcagccact gttccagcgc gttacgctca aaggagatat gaaccacttc
    18181 catatcatgc agttttaacg gtttttgcag cagcagagtc agagtggaag cagtaacgtt
    18241 aacaggaagt tgatactgct gacgacggct gaaaatgccg cccagacgaa ttaccgttaa
    18301 ggctgtttta tcgctttgtt gctgctggag ttgcaggtga cgttgcacaa tcgcgcccag
    18361 gcgggtcaac atcccttgct gttgccaggc atgaccggtc atcaatacat caatttgttg
    18421 ttgtatcaaa ttcaggcttt gcgcgtcata ccacaggcaa tctttcagca aaatgagatc
    18481 taccggggca acggcactgc gaccgctaaa aaaggcgctg gcctgcaata agcggatcgc
    18541 ttttttccag cgacgatccg agacataagg cgcatccggt aatttatcca gttgctggcg
    18601 cagcataaaa atcagctcaa atacatgatc gggcagcgta atttcaccaa tctctttctg
    18661 ccagcgttca tattcttcat ctgtgacctg caaggaggca ggaaccggat tgtcgttttc
    18721 atcctgttga ctggtcagca tggagcggaa attcgcttta tcctgcactt tatctaacca
    18781 cagacgaatc agcatgcggt catataacgc ttccagactg ctgtctgctt ccggcagctc
    18841 gttggaggcc gccaccagca ggcgcatcgg gattttttct acgtgtgcac cgttgcggaa
    18901 ctggcgctcg ttaatggcgg tgagcagggt attaagaatt gccggacccg ctttccagat
    18961 ctcatccaga aagacgattt ctgcttccgg caggtaaccg ctggttaaac gttcatagcg
    19021 cccttcatct tttagcgcct gaatagaaag gggaccaaaa acctcttccg gcgtggagaa
    19081 gcgagtcatc agatattcaa acgcgcgggc attctgaaag gcgaatttta agcgccgggc
    19141 gatcaaactt ttggcaatac ctggcgggcc aaggaggaac acactttcac cacttaatgc
    19201 cgctaataaa cacaagcgga tggcgtggct acgttcataa agcccctttt ccaacgaact
    19261 gctcaggcgg gaaattcttt ccgctaataa atgagggtga gccataatga agtggcgtcc
    19321 tttcgtcaaa agttctgcgt aaattgcgag tatagacgtt tcttgctggt ggctaaaata
    19381 gtctcaaagg gggggtattt ttctttgagc caggttaatg tggccgcatt taggagtacg
    19441 attttgccgt taatcgtgca tactgtgcgc ttttttgtgg gccaagggac taagcacaca
    19501 tttcatattt caacgaaaga ctagtctatg agcactgata ataagcaatc attgcccgcg
    19561 attaccctcg cggcgattgg agttgtctac ggcgatattg gtaccagccc gttatataca
    19621 cttcgtgaat gtttgtccgg ccagtttggt tttggcgttg aacgcgatgc cgtgtttggc
    19681 tttttatcgc tgatcttctg gctgctaatc tttgtggttt ccattaaata tctcaccttc
    19741 gtgatgcggg cagataacgc cggtgaaggg gggatcctga cgttgatgtc gcttgccggg
    19801 cgtaatacgt cggcgcgaac cacatcaatg ctggtgatta tggggctaat cggcggcagc
    19861 tttttctatg gtgaagtcgt cataacaccc gctatttcgg tgatgtccgc cattgaaggt
    19921 ctggaaatcg tcgccccgca gctggatacc tggatagttc ccctctcaat tatcgttctc
    19981 acattattat tcatgattca aaaacatggc acggctatgg tcggtaagct gtttgcgccg
    20041 atcatgctga cctggttttt gattctggca gggctggggt tacgtagcat tattgctaac
    20101 ccggaagtgc tgcatgcact gaatccgatg tgggcggtgc atttcttcct tgaatacaaa
    20161 acggtttctt ttattgcatt aggggcagtg gtgctgtcga ttacgggggt cgaggcgctg
    20221 tatgctgata tggggcactt tggtaagttc cctattcgtc tggcgtggtt caccgtcgta
    20281 ttgccttcct taacccttaa ttacttcggc cagggagcgc tgttgttaaa gaatccggaa
    20341 gcgattaaga acccgttctt cctgttggca ccggactggg cgctgatccc gctgctgatc
    20401 atcgccgcac tggcgacggt aattgcctcg caggcggtta tctctggcgt cttctcattg
    20461 acgcgtcagg cggtacgtct gggatatttg tcgccgatgc gcattattca cacttccgaa
    20521 atggagtcag ggcaaatcta tattcccttt gtgaactgga tgctctatgt cgcggtcgtg
    20581 attgtgattg tcagctttga gcactccagc aacctggcgg cggcgtacgg gattgcggtg
    20641 accggaacca tggtgctgac gtctattctc tcgactaccg tggcacgtca gaactggcac
    20701 tggaataagt attttgttgt gctgatcctg attgctttcc tttgtgtcga tattcccttg
    20761 ttcaccgcta acctcgataa actgctctcc ggcggctggt tgccattgag cctcggtacg
    20821 gtgatgttta tcgtgatgac cacctggaag agcgagcgtt tccgcttgct gcggcggatg
    20881 catgaacatg gtaactctct ggaagcgatg attgcttcgc tggagaaatc accgcccgtt
    20941 cgcgtgcctg ggaccgcggt gtatatgtcg cgtgcaatca acgtcattcc ctttgcgctg
    21001 atgcataacc tgaaacataa caaggtattg catgagcggg tgattctgtt aactctgcgc
    21061 accgaagacg cgccatatgt ccataacgtc cgtcgggtac agattgaaca actgtcgccc
    21121 actttctggc gcgtggtggc aagttatggt tggcgagaaa cgccaaacgt ggaagaagtt
    21181 ttccaccgct gcggtctgga aggattaagt tgccggatga tggaaacctc cttctttatg
    21241 tcgcatgagt cgttgatcct cggcaaacgc ccgtggtatt tgcgtctgcg cggcaagctg
    21301 tacttgctgc tgcaacgtaa tgcgctgcgt gcgccggatc aatttgaaat cccaccaaac
    21361 agggttatcg aactgggtac tcaggtcgaa atctaacgcc agacgcctcc tttctccata
    21421 agggggcgtt tttgttttca tggttaatca ccatgtaaaa cgtttcgagg ttgatcacat
    21481 ttccgtaacg tcacgatggt tttcccaact cagtcaggat taaactgcgg gtcagcgaaa
    21541 cgtttcgctg atggagaaaa aaatgaaaaa aggcaccgtt cttaattctg atatttcatc
    21601 ggtgatctcc cgtctgggac ataccgatac gctggtggtg tgtgatgctg gtttacccat
    21661 ccccaaaagt acaacgcgta tcgatatggc attaacccag ggtgtacctt cttttatgca
    21721 ggtgctgggc gtcgtcacaa atgaaatgca ggtcgaggcg gccattatcg cggaagagat
    21781 caaacaacat aatccgcatc tccacgaaac gttgctcact caccttgagc agctgcaaca
    21841 acaccaggga aataccattg aaattcgtta caccacgcat gaacaattca aacaacaaac
    21901 cgcagaaagt caggcggtaa ttcgcagcgg agaatgttct ccgtatgcga atatcattct
    21961 ctgtgctggc gtgacgttct gaggccgtca tggaagcatt acttcagctt aaaggcatcg
    22021 ataaagcctt cccgggcgta aaagccctct cgggcgcagc gttaaatgtc tatccgggcc
    22081 gtgtgatggc gctggtgggc gaaaacggtg cgggtaaatc caccatgatg aaagtgctta
    22141 cgggtatcta tactcgcgat gccggtacgc ttttatggct ggggaaagaa acgacgttta
    22201 ccgggccgaa ctcttcccag gaagccggga ttgggattat ccatcaggaa ctgaacctga
    22261 tcccgcagtt gaccattgcc gaaaacattt tcctcggtcg tgagtttgtt aatcgctttg
    22321 gcaaaattga ctggaaaacc atgtatgccg aagcggataa attgctggct aaacttaacc
    22381 tgcgctttaa aagcgacaag ctggtgggcg atctttccat cggtgaccag caaatggttg
    22441 aaatagccaa agtgctgagc tttgagtcga aagtcatcat tatggatgaa ccgaccgatg
    22501 cgctgaccga taccgaaacc gaatccttgt tccgcgtcat ccgcgagctg aaatcgcaag
    22561 gccgcggtat tgtctatatt tcccaccgca tgaaagaaat cttcgagatt tgcgatgacg
    22621 ttaccgtttt tcgtgacggg caatttattg ctgagcgtga agtagcatca ctgaccgaag
    22681 attcgctgat tgagatgatg gtgggccgca agctggaaga tcaatatcca cacctggaca
    22741 aagcgccggg agatattcgc ctgaaagtcg ataatctctg cggacctggc gttaacgatg
    22801 tctcttttac tttacgcaaa ggcgaaatcc ttggcgtctc tggtttgatg ggcgcaggtc
    22861 gtactgaact gatgaaagtg ctctacggcg cactgccgcg caccagcggt tacgtcaccc
    22921 tggatgggca tgaagtcgtt acccgttcac cacaggatgg cctggcaaac ggcattgtgt
    22981 atatctccga agaccgtaaa cgtgacggtt tagtgctggg catgtcagta aaagagaata
    23041 tgtcgctgac cgcgctgcgc tacttcagcc gcgctggcgg cagtttgaag catgccgatg
    23101 aacagcaggc agtaagtgat ttcattcgtc tgtttaatgt gaaaacgccg tcgatggaac
    23161 aggcaattgg tctgctttcc ggtggcaatc agcaaaaagt ggcgattgcc cgtggtctga
    23221 tgacacgccc caaagtgttg atcctcgatg agcctacccg tggcgtagat gtcggcgcga
    23281 aaaaagagat ctatcaactg attaaccagt tcaaagccga tggcttgagc atcattctgg
    23341 tgtcatcgga gatgccagaa gtattaggca tgagcgatcg catcatcgtc atgcatgaag
    23401 ggcatctcag cggggaattt actcgtgagc aggccaccca ggaagtgtta atggctgccg
    23461 ctgtgggcaa gcttaatcgc gtgaatcagg agtaaaaaaa tgacaaccca gactgtctct
    23521 ggtcgccgtt atttcacgaa agcgtggctg atggagcaga aatcgcttat cgctctgctg
    23581 gtgctgatcg cgattgtctc gacgttaagt ccgaactttt tcaccatcaa taacttattc
    23641 aatattctcc agcaaacctc ggtgaacgcc attatggcag tcgggatgac gctggtgatc
    23701 ctgacgtcgg gcatcgactt gtcggtaggt tctctgttgg cgctgaccgg cgcagttgct
    23761 gcatctatcg ttggcattga agtcaatgcg ctggtggctg ttgctgctgc tctcgcgtta
    23821 ggtgccgcaa ttggtgcggt aaccggggtg attgtagcga aaggtcgcgt ccaggcgttt
    23881 atcgctacgc tggttatgat gcttttactg cgcggcgtga ccatggttta taccaacggt
    23941 agcccagtga ataccggctt tactgagaac gccgatctgt ttggctggtt tggtattggt
    24001 cgtccgctgg gcgtaccgac gccagtctgg atcatgggta ttgtcttcct cgcggcctgg
    24061 tacatgctgc atcacacgcg tttggggcgt tacatctacg cgctgggcgg caacgaagcg
    24121 gcaacgcgtc tttctggtat caacgtcaat aaaatcaaaa tcatcgtcta ttctctttgt
    24181 ggtctgctgg catcgctggc cgggatcatt gaagtggcgc gtctctcctc cgcacaaccc
    24241 acggcgggga ctggctatga gctggatgct attgctgcgg tggttctggg cggtacgagt
    24301 ctggcgggcg gaaaaggtcg cattgttgga acgttgatcg gtgcattaat tcttggcttc
    24361 cttaataatg gattgaattt gttaggtgtt tcctcctatt accagatgat cgtcaaagcg
    24421 gtggtgattt tgctggcggt gctggtagac aacaaaaagc agtaataacg actacaggac
    24481 atcttgaata tgaacatgaa aaaactggct accctggttt ccgctgttgc gctaagcgcc
    24541 accgtcagtg cgaatgcgat ggcaaaagac accatcgcgc tggtggtctc cacgcttaac
    24601 aacccgttct ttgtatcgct gaaagatggc gcgcagaaag aggcggataa acttggttat
    24661 aacctggtgg tgctggactc ccagaacaac ccggcgaaag agctggcgaa cgtacaggac
    24721 ttaaccgttc gcggcaccaa aattctgctg attaacccga ccgactccga cgcagtgggt
    24781 aatgctgtga agatggctaa ccaggcgaac atcccggtta tcactcttga ccgccaggca
    24841 acgaaaggtg aagtggtgag ccacattgct tctgataacg tacttggcgg caaaatcgcc
    24901 ggtgattaca tcgcgaagaa agcgggcgaa ggtgccaaag ttatcgagct gcaaggcatt
    24961 gctggtacat ccgcagcccg tgaacgtggc gaaggcttcc agcaggctgt tgctgctcac
    25021 aagtttaatg ttcttgccag ccagccagca gattttgatc gcaccaaagg tttgaacgta
    25081 atgcagaacc tgttgaccgc acatccggat gttcaggctg tattcgcgca gaatgatgaa
    25141 atggcgctgg gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc
    25201 ggatttgatg gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact
    25261 atcgctcagc tacccgatca gattggcgcg aaaggcgtcg aaaccgcaga taaagtactg
    25321 aaaggcgaga aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag
    25381 ttttaatcag gttgtatgac ctgatggtga cataaatacg gcatcgacag atgaacgtgt
    25441 aatataaaga aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc
    25501 cgaatatgca aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc
    25561 ttaatcttca atcttttcct accccaggcg aaaccgtaac cggtaaccac tatcaggttg
    25621 catttggcgg caaaggcgcg aatcaggctg tggctgctgg gcgtagcggt gcgaatatcg
    25681 cgtttattgc ctgtactggc gatgacagca ttggcgagag cgttcgccag cagctcgcca
    25741 ctgataacat cgatatttct ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc
    25801 tgatttttgt taatggcgaa ggtgagaatg tcattggtat tcatgccggc gctaatgctg
    25861 ccctttcccc ggcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcattat
    25921 taatgcagct ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa
    25981 ataagactat cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg
    26041 cgctggtgga cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg
    26101 ttgaaaatga tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaaa ggtatccgta
    26161 ctgtactgat tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc
    26221 gcgttcctgg attccgggtg caggctgtcg ataccattgc tgccggagat acctttaacg
    26281 gcgcgttaat cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc
    26341 atgctgccgc tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg
    26401 aagagatcga cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg
    26461 cccgcctggc gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg
    26521 tcagtgaagc gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat
    26581 cagccctggc gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg
    26641 ccagtaccaa tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac
    26701 gcggttatag tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaatc
    26761 tggaaacgct gatgcaaaaa cgcgttgatg gcttgctgtt actgtgcacc gaaacgcatc
    26821 aaccttcgcg tgaaatcatg caacgttatc cgacagtgcc tactgtgatg atggactggg
    26881 ctccgttcga tggcgacagc gatcttattc aggataactc gttgctgggc ggagacttag
    26941 caacgcaata tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg
    27001 ataaaactcc ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc
    27061 tcaacattcc tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg
    27121 ccatgcgcca actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg
    27181 ctatggctgt tggcgtttac caggcgctgt atcaggcaga gttacaggtt ccgcaggata
    27241 tcgcggtgat tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca
    27301 ctatccacca accgaaagat gaactggggg aactggcgat tgatgtactc atccatcgga
    27361 taacccagcc gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg
    27421 gttcggctta gatttacgct gtcttttgat caaattatta ccatcagttg ttttcagaag
    27481 catgaacatt gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa
    27541 ttgttcgact gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac
    27601 agcaacgcct aaactgatag acagttgctg cgtgaccgcc agaacactgt taccgctgct
    27661 ggcgttgtca tcggtcagat cggcaagtgt gatggtattc atcgcggtaa attgcgtcga
    27721 catagccatc cctaatataa acaacggcaa gatcagcatc catatcgcca ttgccggtga
    27781 ttgcaaatag aactgagcga tcattagccc aataatcacc gtgatcccaa ctaacgtatg
    27841 gcgatagccc agacgacgta agacttgggt aaccatcgat tttgcaataa tggaacctaa
    27901 cgctgtcggt gccatcatac agccggcaat aaaagcctga taaccaaatc ctacctgtaa
    27961 catcaatggc ataaggaacg gtacacaacc ggtccccaga cgggttgcaa tattgcctac
    28021 gataccgatc gagaaagtgc gggttttaaa taaatctaat gaaattaatg ggtttggcgt
    28081 gcgtcgtgca tggagaatat agagaagcag taacccgatg ctggtgacaa ttaccgtcaa
    28141 ggcaatccaa ctggcgacaa tcttttcccc gaatagctct attccgcttg agaagagaac
    28201 aaggctaagg ccaaacagca aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa
    28261 attgggcata tgtttgcgcg cgtaaagaag gcccgcaata cctatgggga tattgattaa
    28321 aaatatccag tgccaggttg cccaggtgac cagcacgccg ccaagaacgg ggcctaaaat
    28381 tggccccacc agacccggca tggcgacaaa attcaataca ggaagaagct cattacgagg
    28441 ataagcgcgc agtaaggcca gccgagcaac aggcatcatc attgcgccgc ctatcccctg
    28501 aataacccgg aagacaacca gctgtggtag cgagttagaa agtgcgcagg ccagagaacc
    28561 caacgtgaac agactcacgg caagagtaaa tatgcgacgc gtaccgaagc gatcggctag
    28621 ccatccgctt accggaataa gcatcgccac cgtcagcgta taactgatga tggctgattg
    28681 catcgcgaga ggagaacgat taaggctatg agcgattgcg ggtaaggcgg tattcagaat
    28741 agtggcatca agtgcctgca tgaagaaggc catcgccgcg atccacggca aacccgccat
    28801 actgcgcttc tttttatcgc tcattcaatg tcctgttatc ggggtgtcac ttatcaggtg
    28861 agcgtagcag cgcctgacaa gctttaaatg ccgcgtcgcc atcgctttgg ataatcgcgt
    28921 caacaatcgc ctggtgcaga tccagcttta tcactgtgtc gctggtaatt gacgtgaagt
    28981 aagtgtgata aaccgaatgg aagagcgagg cgaatgaggt caaaaacgga ttggcgctca
    29041 tttcatagat atgctcatgc caggccatat cgacctcaat ccagcgttca cggcgaaagt
    29101 tctcttttaa tgccaccatt tcggccatta acgtattgag atgcgccttc tgttccgcgg
    29161 tgccaaccat tgctgccagt aggcaagctt gcggctccag acagatacgc ataaccagaa
    29221 agtgatcgat gacctgatga aagttctctt ctgtcatcca ccaggtaagc aattcctgat
    29281 caagaaaatt ccagtttgat tgtggcatga cccgggtacc aattcgcggt cgcggtaaaa
    29341 ccatcccttt tgccgttaac gttttgaccg cttcgcgtac cgctgtacga ctcactccaa
    29401 attgctcgcc cagctcaatc tcaccaggca aaatggtgcc gggttcatat tcacctttta
    29461 agatgcgttg cgccagcttc tcagccagaa catacgaaag gtttttctgt gcagctaact
    29521 gttgtgcgct taaaggcatt actaatcttc ctttttcttt ttattcctcc ttagtatgcc
    29581 accaggaagt gtgattacgg ttgcaaaaac ggcaaattgc ttgttttatg gcacattaac
    29641 ggggcttttg ctgaaaaaat gcgcggtcag aaaattattt taaatttcct cttgtcaggc
    29701 cggaataact ccctataatg cgccaccact gacacggaac aacggcaaac acgccgccgg
    29761 gttagcgggg ttctcctgag aattccggca gagaaagcaa aaataaatgc ttgactctgt
    29821 agcgggaa
//
LOCUS       DAPHCV010000060        29711 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.060, whole genome shotgun sequence.
ACCESSION   DAPHCV010000060 DAPHCV010000000
VERSION     DAPHCV010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.060"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            20..670
                     /gene="zinT"
                     /locus_tag="RH033_002615"
     CDS             20..670
                     /gene="zinT"
                     /locus_tag="RH033_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HDV2991785.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYV
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(1340..1429)
                     /locus_tag="RH033_002616"
     tRNA            complement(1340..1429)
                     /locus_tag="RH033_002616"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1393..1395),aa:Ser,seq:cga)
     gene            1523..2320
                     /gene="mtfA"
                     /locus_tag="RH033_002617"
     CDS             1523..2320
                     /gene="mtfA"
                     /locus_tag="RH033_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HDV2991786.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2421..2496
                     /locus_tag="RH033_002618"
     tRNA            2421..2496
                     /locus_tag="RH033_002618"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2454..2456,aa:Asn,seq:gtt)
     gene            2658..3920
                     /locus_tag="RH033_002619"
     CDS             2658..3920
                     /locus_tag="RH033_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDV2991787.1"
                     /translation="MFLTDAKIRTLKPSDKPFKVSDSHGLYLLVKPGGSRHWYLKYRI
                     SGKESRIALGAYQAISLSDARQQREGIRKMLALNINPVQQRAAERGSRTPEKVFKNLA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLLKGIEEKGLLEV
                     ASRTRQHLSNIMRHAVHQELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGRELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAI
                     MQWWSDYLEACRESYAPPYTIGKNKFIP"
     gene            complement(4114..5418)
                     /gene="ybtS"
                     /locus_tag="RH033_002620"
     CDS             complement(4114..5418)
                     /gene="ybtS"
                     /locus_tag="RH033_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salicylate synthase
                     YbtS"
                     /protein_id="HDV2991788.1"
                     /translation="MKISEFLHLALPEEQWLPTISGVLRQFAEEECYVYERQPCWYLG
                     KGCQARLHIDADGTQATFIDDAGEQKWAVDSIADCARRFMAHPQVKGRRVYGQVGFNF
                     AAHARGIAFNAGEWPLLTLTVPREELIFEKGNVTVYADSADGCRRLCEWVKEAGTTTQ
                     NAPLAVDTALNGEAYKQQVARAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGRQA
                     NTPVRSFMFRQEGREALGFSPELVMSVTGNKVVTEPLAGTRDRMGNPEHNKAKEAELL
                     HDSKEVLEHILSVKEAIAELEAVCQPGSVVVEDLMSVRQRGSVQHLGSGVSGQLSENK
                     DAWDAFTVLFPSITASGIPKNAALNAIMQIEKTPRELYSGAILLLDDTRFDAALVLRS
                     VFQDSQRSWIQAGAGIIAQSTPERELTETREKLASIAPYLMV"
     gene            complement(5446..6726)
                     /gene="ybtX"
                     /locus_tag="RH033_002621"
     CDS             complement(5446..6726)
                     /gene="ybtX"
                     /locus_tag="RH033_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin-associated zinc MFS transporter
                     YbtX"
                     /protein_id="HDV2991789.1"
                     /translation="MSDVQSNVKPLTLTTGRVIFAIAGVYVTQSLVSALSMQSLPALV
                     RAAGGSLALAGATTLFMLPWALKFIWAPWIERWRLPPGSQERRSRMLILRGQVALAAI
                     LTIVAAIGWFGREGGFPDTQIVALFVLFMVAGTVASTIDIASDGFCVDQLTRTGYGWG
                     NSVQVGGSYLGMMCGGGVFLMLSAASGWPVAMLMMAVLIMALSLPLWRITEPTRTATI
                     PHVPALGYALRRKQARLGLLLVLMLNSGMRFVLPLLAPLLLDHGLSMSALGALFSGGN
                     IAAGIAGTLAGGLLMKYTSPGRALLTAYGVQGIALLAVVMTLMMAPGHLLLQILQCLV
                     LVQSISLACALVCLYATLMSLSSPLQAGVDFTLFQCTDAAIAILAGVIGGVVAQHFGY
                     AACFLFAGAFTLLAAWVAYIRLHSARELMTSAID"
     gene            complement(6719..8521)
                     /gene="ybtQ"
                     /locus_tag="RH033_002622"
     CDS             complement(6719..8521)
                     /gene="ybtQ"
                     /locus_tag="RH033_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtQ"
                     /protein_id="HDV2991790.1"
                     /translation="MKDNNPADNLAWRVIWRQLISSVGSQARMLRRSMLALLLAAFMQ
                     GIAFACLYPIIDALLRGDAPQLLNWAMAFSVAAIVTLVLRWYGLGFEYRGHLAQATHE
                     LRLRLGEQLRRVPLEKLQRGRAGEMNALLLGSVDENLNYVIAIANILLLTIVTPLTAS
                     LATLWIDWRLGLVMLLIFPLLVPFYYWRRPAMRRQMQTLGEAHQRLSGDIVEFAQGMM
                     VLRTCGSDADKSRALLAHFNALENLQTRTHRQGAGATMLIASVVELGLQVVVLSGIVW
                     VVTGTLNLAFLIAAVAMIMRFAEPMAMFISYTSVVELIASALQRIEQFMAIAPLPVAE
                     QSEMPERYDIRFDNVSYRYEEGDGYALNHVSLTFPAASMSALVGASGAGKTTVTKLLM
                     RYADPQQGQISIGGVDIRRLTPEQLNSLISVVFQDVWLFDDTLLANIRIARPQATRQE
                     VEEAARAAQCLEFISRLPQGWLTPMGEMGGQLSGGERQRISIARALLKNAPVVILDEP
                     TAALDIESELAVQKAIDNLVHNRTVIIIAHRLSTIAGAGNILVMEEGQVVEQGTHAQL
                     LSHHGRYQALWQAQMAARVWRDDGVSASGEWVHE"
     gene            complement(8508..10220)
                     /gene="ybtP"
                     /locus_tag="RH033_002623"
     CDS             complement(8508..10220)
                     /gene="ybtP"
                     /locus_tag="RH033_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtP"
                     /protein_id="HDV2991791.1"
                     /translation="MALAGLAALTSLGALLFLAWSLRDIRATPDAIPAWPLGGVIGCV
                     VLTFVLRLQAFNTSHYAAFHLENILRSRLARKALQLPPGVLQQMGSGSVAKVMLDDVK
                     SLHIFVADSTPLYARAIIMPLATIVILFWLDWRLAIATLGVLAFGSVILVLARQRSEN
                     MAQRYHKAREQVSAAVIEFVQAMPVVRTFDSGSTSFLRYQRALEEWVDVLKTWYRKAG
                     FSARFSFSILNPLPTLFVLIWSGYGLLHYGSFDFIAWVAVLLLGSGMAEAVMPMMMLN
                     NLVAQTRLSIQRIYQVLAMPELSLPQSDQQPQEASITFEQVSFHYPQARTGAALQEVS
                     FHVPAGQIVALVGPSGAGKSTVARLLLRYADPDKGHIRIGGVDLRDMQTDTLMKQLSF
                     VFQDNFLFADTIANNIRLGAPDTPLEAVIAAARVAQAHDFISALPEGYNTRVGERGVF
                     LSGGQRQRITIARALLQDRPILVLDEATAFADPENEAALIKALAAAMRGRTVIMVAHR
                     LSMVTQADVILLFSDGQLREMGNHTQLLAQGGLYQRLWQHYQQAQHWVPGGTQEEVVE
                     NERQ"
     gene            10477..11436
                     /gene="ybtA"
                     /locus_tag="RH033_002624"
     CDS             10477..11436
                     /gene="ybtA"
                     /locus_tag="RH033_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin transcriptional regulator YbtA"
                     /protein_id="HDV2991792.1"
                     /translation="MTESPQTQSEISIHQLVVGKPANDGNIPAQCELLRCSLQEGMDI
                     LLWRGHFARPETLQLHDDLGRINFSCILEGTSRFAIQGLRRHTDWELARNRHYITHTP
                     DCRGSASYCGRFESITLSFSPETLALWVPDISAVIKNKIDSHCCCQQQRCNAETHLTA
                     QALRHALMRMHGGFSHEQKPSTLWLQGQSLVMLSLVLDEHREDASCLSCHFNPMERQK
                     LLRAKDLLLADLTQAPGVAELARESGLSVLKIKRGFRVLFNNSVYGLFQAERMQEARR
                     RLANGNTSVMTVAADLGYANASHFSAAFQKQFGVTPSTFKRGM"
     gene            11627..17734
                     /gene="irp2"
                     /locus_tag="RH033_002625"
     CDS             11627..17734
                     /gene="irp2"
                     /locus_tag="RH033_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin non-ribosomal peptide synthetase
                     HMWP2"
                     /protein_id="HDV2991793.1"
                     /translation="MISGAPSQDSLLPDNRHAADYQQLRERLIQELNLTPQQLHEESN
                     LIQAGLDSIRLMRWLHWFRKNGYRLTLRELYAAPTLAAWNQLMLSRSPENAEEETPPD
                     ESSWPNMTESTPFPLTPVQHAYLTGRMPGQTLGGVGCHLYQEFEGHCLTASQLEQAIT
                     TLLQRHPMLHIAFRPDGQQVWLPQPYWNGVTVHDLRHNDAESRQAYLDALRQRLSHRL
                     LRVEIGETFDFQLTLLPDNRHRLHVNIDLLIMDASSFTLFFDELNALLAGESLPAIDT
                     RYDFRSYLLHQQKINQPLRDVARAYWLAKASTLPPAPVLPLACEPATLREVRNTRRRM
                     IVPATRWHAFSNRAGEYGVTPTMALATCFSAVLARWGGLTRLLLNITLFDRQPLQPAV
                     GAMLADFTNILLLDTACDGDTVSNLARKNQLTFTEDWEHRHWSGVELLRELKRQQRYP
                     HGAPVVFTSNLGRSLYSSRAESPLGEPEWGISQTPQVWIDHLAFEHHGEVWLQWDSND
                     ALFPPALVETLFDAYCQLINQLCDDESAWQKPFADMMPASQRAIRERVNATGAPIPEG
                     LLHEGIFRIALQQPQALAVTDMRYQWNYHELTDYARRCAGRLIECGVQPGDNVAITMS
                     KGAGQLVAVLAVLLAGAVYVPVSLDQPAARREKIYADASVRLVLICQHDASAGSDDIP
                     ALAWQQAIEAEPIANPVVRAPTQPAYIIYTSGSTGTPKGVVISHRGALNTCCDINTRY
                     QVGPHDRVLALSALHFDLSVYDIFGVLRAGGALVMVMENQRRDPHAWCELIQRHQVTL
                     WNSVPALFDMLLTWCEGFADATPENLRAVMLSGDWIGLDLPARYRAFRPQGQFIAMGG
                     ATEASIWSNACEIHDVPAHWRSIPYGFPLTNQRYRVVDEQGRDCPDWVPGELWIGGIG
                     VAEGYFNDPLRSEQQFLTLPDERWYRTGDLGCYWPDGTIEFLGRRDKQVKVGGYRIEL
                     GEIESALSQLVGVKQATVLAIGEKEKTLAAYVVPQGEAFCVTDHRNPVLPQAWHTLAG
                     TLPCCAISPEISAEQVADFLQHRLLKLKPGHTAGADPLPLMNSLAIQPRWQAVVERWL
                     AFLVTQRRLKPAAEGYQVCAGEEREDEHPHFSGHDLTLSQILRGARNELSLLNDAQWS
                     PESLAFNHPASAPYIQELATICQQLAQRLQRPVRLLEVGTRTGRAAESLLAQLNAGQI
                     EYVGLEQSQEMLLSARQRLAPWPGARLSLWNADTLAAHAHSADIIWLNNALHRLLPED
                     PGLLATLQQLAVPGALLYVMEFRQLTPSALLSTLLLTNGQPEALLHNSADWAALFSAA
                     GFNCQHGDEVAGLQRFLVQCPDRQVRRDPRQLQAALAGRLPGWMVPQRIVFLDALPLT
                     ANGKIDYQALKRRHTPEAENPAEADLPQGDIEKQVAALWQQLLSTDNVTRETDFFQQG
                     GDSLLATRLTGQLHQAGYEAQLSDLFNHPRLADFAATLRKTDVPVEQPFVHSLEDRYQ
                     PFALTDVQQAYLVGRQPGFALGGVGSHLFVEFEIADLDLTRLETVWNRLIARHDMLRA
                     IVRDGQQQVLEQTPPWVIPAHTLHTPEEALRVREKLAHQVLNPEVWPVFDLQVGYVDG
                     MPARLWLCLDNLLLDGLSMQILLAELEHGYRYPQQLLPPLPVTFRDYLQQPSLQSPNP
                     DSLAWWQAQLDDIPPAPALPLRCLPQEVETPRFARLNGALDSTRWHRLKKRAADAHLT
                     PSAVLLSVWSTVLSAWSAQPDFTLNLTLFDRRPLHPQINQILGDFTSLMLLSWHPGES
                     WLHSAQSLQQRLSQNLNHRDVSAIRVMRQLAQRQNVPAVPMPVVFTSALGFEQDNFLA
                     RRNLLKPVWGISQTPQVWLDHQIYESEGELRFNWDFVAALFPAGQVERQFEQYCALLN
                     RMAEDESGWQLPLAALVPPVKHAGQCAERSPRVCPEHSQPHIAADESTVSLICDAFRE
                     VVGESVTPAENFFEAGATSLNLVQLHVLLQRHEFSTLTLLDLFTHPSPAALADYLAGV
                     ATVEKTQRPRPVRRRQRRI"
     gene            17822..23755
                     /gene="irp1"
                     /locus_tag="RH033_002626"
                     /pseudo
     CDS             17822..23755
                     /gene="irp1"
                     /locus_tag="RH033_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227767.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin polyketide synthase HMWP1"
     gene            23941..25041
                     /gene="ybtU"
                     /locus_tag="RH033_002627"
     CDS             23941..25041
                     /gene="ybtU"
                     /locus_tag="RH033_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis oxidoreductase YbtU"
                     /protein_id="HDV2991794.1"
                     /translation="MMPSASPKQRVLIVGAKFGEMYLNAFMQPPEGLELVGLLAQGSA
                     RSRELAHAFGIPLYTSPEQITRMPDIACIVVRSTVAGGTGTQLAKHFLTRGVHVIQEH
                     PLHPDDISSLQTLAQEQGCCYWVNTFYPHTRAGRTWLRDAQQLRRCLAKTPPVVHATT
                     SRQLLYSTLDLLLLALGVDAAAVECDVVGSFSDFHCLRLFWPEGEACLLLQRYLDPDD
                     PDMHSLIMHRLLLGWPEGHLSLEASYGPVIWSSSLFVADHQENAHSLYRRPEILRDLP
                     GLTRSAAPLSWRDCCETVGPEGVSWLLHQLRSHLAGEHPPAACQSVHQIALSRLWQQI
                     LRKTGNAEIRRLTPPHHDRLAGFYNDDDKEAL"
     gene            25038..25841
                     /gene="ybtT"
                     /locus_tag="RH033_002628"
     CDS             25038..25841
                     /gene="ybtT"
                     /locus_tag="RH033_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis thioesterase YbtT"
                     /protein_id="HDV2991795.1"
                     /translation="MTQSAMCIPLWPARNGNTAHLVMCPFAGGSSSAFRHWQAEQLAD
                     CALSLVTWPGRDRLRHLEPLRSITQLAALLANELEASVSPDTPLLLAGHSMGAQVAFE
                     TCRLLEQRGLAPQGLIISGCHAPHLHSERQLSHRDDADFIAELIDIGGCSPELRENQE
                     LMSLFLPLLRADFYATESYHYDSPDVCPPLRTPALLLCGSHDREASWQQVDAWRQWLS
                     HVTGPVVIDGDHFYPIQQARSFFTQIVRHFPHAFSAMTALQKQPSTSER"
     gene            25845..27422
                     /gene="ybtE"
                     /locus_tag="RH033_002629"
     CDS             25845..27422
                     /gene="ybtE"
                     /locus_tag="RH033_002629"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salycil-AMP ligase
                     YbtE"
                     /protein_id="HDV2991796.1"
                     /translation="MNSSFESLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSA
                     LDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTLLFALLRLGVIPVLAMPSQRALD
                     IDALIELAQPVAYVIHGENHAELARQMAHKHACLRHVLVAGETVSDDFTPLFSLHGER
                     QAWPQPDVSATALLLLSGGTTGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLP
                     VAHNFPLACPGILGTLACGGKVVLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQA
                     REWEDSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMAEGLLCFTRLDDPHA
                     TILHSQGRPLSPLDEIRIVDQDENDVAPGETGQLLTRGPYTISGYYRAPAHNAQAFTA
                     QGFYRTGDNVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAA
                     PDTLLGERICAFIIAQQVPTDYQQLRQQLTRMGLSAWKIPDQIEFLDHWPLTAVGKID
                     KKRLTALAVDRYRHSAQ"
     gene            27553..29574
                     /gene="fyuA"
                     /locus_tag="RH033_002630"
     CDS             27553..29574
                     /gene="fyuA"
                     /locus_tag="RH033_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore yersiniabactin receptor FyuA"
                     /protein_id="HDV2991797.1"
                     /translation="MKMTRLYPLALGGLLLPAIANAQTSQQDESTLVVTASKQSSRSA
                     SANNVSSTVVSAPELSDAGVTASDKLPRVLPGLNIENSGNMLFSTISLRGVSSAQDFY
                     NPAVTLYVDGVPQLSTNTIQALTDVQSVELLRGPQGTLYGKSAQGGIINIVTQQPDST
                     PRGYIEGGVSSRDSYRSKFNLSGPIQDGLLYGSVTLLRQVDDGDMINPATGSDDLGGT
                     RASIGNVKLRLAPDDQPWEMGFAASRECTRATQDAYVGWNDIKGRKLSISDGSPDPYM
                     RRCTDSQTLSGKYTTDDWVFNLISAWQQQHYSRTFPSGSLIVNMPQRWNQDVQELRAA
                     TLGDARTVDMVFGLYRQNTREKLNSAYDMPTMPYLSSTGYTTAETLAAYSDLTWHLTD
                     RFDIGGGVRFSHDKSSTQYHGSMLGNPFGDQGKSNDDQVLGQLSAGYMLTDDWRVYTR
                     VAQGYKPSGYNIVPTAGLDAKPFVAEKSINYELGTRYETADVTLQAATFYTHTKDMQL
                     YSGPVGMQTLSNAGKADATGVELEAKWRFAPGWSWDINGNVIRSEFTNDSELYHDNRV
                     PFVPRYGAGSSVNGVIDTRYGALMPRLAVNLVGPHYFDGDNQLRQGTYATLDSSLGWQ
                     ATERMNISVYVDNLFDRRYRTYGYMNGSSAVAQVNMGRTVGINTRIDFF"
BASE COUNT         6538 a         8812 c         8080 g         6281 t
ORIGIN      
        1 aactctggag gaaactgttt tggcgattcg tcttcataaa ctggctgttg ctttaggtgt
       61 ctttattgtt agcgctcctg ccttttcgca tggtcatcac tcacacggca aacccttaac
      121 agaggtcgaa caaaaagctg ctaatggtgt ttttgatgat gccaatgtac aaaaccgaac
      181 gctcagtgac tgggatggag tctggcaatc cgtttatcct ttactgcaaa gtggcaaact
      241 tgaccccgtc tttcagaaga aagcggatgc agataaaacc aaaacatttg ctgaaattaa
      301 agattattat cgcaaaggtt atgtgacaga catcgagatg attggcattg aggacggcat
      361 tgtcgaattc catagaaata atgaaacaac atcctgtaaa tatgattacg atggatacaa
      421 aatactcacc tataaatcag gcaagaaagg cgttcgttac ttattcgaat gtaaagatcc
      481 tgaaagcaaa gcccctaaat atatacaatt tagcgatcat ataattgcac cacgaaaatc
      541 atctcatttt cacattttta tgggtaatga ttcccaacag tctttattga atgaaatgga
      601 aaactggcca acgtattatc cctatcagtt gagtagcgaa gaagtggtcg aggagatgat
      661 gtctcattga tttttttgcg ctgacttcag ttatttaaca gaacagtgcc tggtagtaac
      721 aacaataatt tgccaagaca aataagtaag acaaactcag acacgctcat acttgagtcg
      781 tgtctttttt attatgtctg tagaaaaaaa atgcgaaaaa ggcgaacaat aaatacacaa
      841 ctcagccata cagactggca gcacaaatgt ttccaggagc aagtcatctg tcatggtaaa
      901 gctaaccaga aatataggtt aattttggtg tttaaacctt ttttacttgt tagtatgata
      961 caaaggcttt caaaaaagct gggaaattca acaactcttg gaaattatgg actttgtccc
     1021 gcggctttaa tctgacggcc gcgttccttt tttgactcaa ttattgaccc cttctctacg
     1081 caacttcagt tcccgccagc aactttgcgg cagtttcata ggatcaatgt ctaaaagaat
     1141 aatggtgacc gataagaaaa cgactgaata actgtagatt ttcgctcgaa accttcctgt
     1201 cagctccata gcgaatcaag tgctgaatgt cacagtatcg aacagaaaac agtgacgatc
     1261 taacccttca agaatattct acgattgttc tgtttaggaa aagcaaggcg ggaagtcagg
     1321 agataagtca ttgataaagt ggcggagaga gggggatttg aacccccggt agagttgccc
     1381 ctactccggt tttcgagacc ggtccgttca gccgctccgg catctctccg ttcagatggt
     1441 tgccatgatg tcaggaaatt tggcatttta acagtccctg tccgtgcaat tttgttcaag
     1501 tgacgagttt gcgagcaaaa cgatgattaa gtggccctgg aaagtacaag aatcagcaca
     1561 tcaaactgcc cttccctggc aggaagcact atcgatcccc cttttaacgg gtctgacgga
     1621 acaggaacaa agcaaattag tcactcttgc cgaacgtttt ttacagcaaa agcggcttgt
     1681 tcctttacag ggctttgaac tggattcatt aagaagctgc cggatagcac ttctattttg
     1741 cctgcccgtt ctggagttag gactggaatg gctggatggt tttcatgaag tcttaattta
     1801 tcctgcgcca tttgtggtcg atgatgaatg ggaagacgat atcggtctgg tgcataacca
     1861 acgtattgtt cagtcaggtc agagctggca gcaagggcct atcgttttga actggttgga
     1921 tatacaagat tcttttgatg catctggttt taacctgatt attcatgaag tcgctcataa
     1981 gctggacacc cgtaacggcg atcgcgccag cggagttccc tttatttcgt tgcgtgaggt
     2041 tgctggctgg gaacacgatc ttcatgctgc aatgaacaac attcaggaag aaatcgaatt
     2101 agttggtgag aatgcggcga gcattgatgc ttatgctgcc agtgatcctg ctgaatgttt
     2161 tgccgtactt tctgaatatt tctttagcgc cccagaactt tttgctcctc gtttcccttc
     2221 attgtggcaa cgtttctgtc aattttatca acaagatcct ttgcagagac tgcatcacgc
     2281 taatgataca gactcgtttt cggcgacgaa tgttcattaa ttaacaactt tgcagattaa
     2341 ttaaccaatt gaaatggctt atgaaattta gtgttgacag acaagatacc gctaagtaat
     2401 atgcgctccg ttcacacgat tcctctgtag ttcagtcggt agaacggcgg actgttaatc
     2461 cgtatgtcac tggttcgagt ccagtcagag gagccaattt tctgttttca tgcatccttg
     2521 caaatcttta tctgatgttg attcaacagg ttagtgtgaa aactcttctc ggtgcatttt
     2581 gattttaccc tacgcatcgg gaaaaattgg tggtcaaatc tggggtcagg ttagttcgat
     2641 aatggagtgc cccccatatg ttccttaccg acgcaaaaat ccgcaccctc aagccttctg
     2701 ataaaccctt taaagtctcc gattctcacg gtctgtatct gctggtcaag ccgggtggct
     2761 cccgccactg gtatctcaaa taccgtatta gcggtaaaga atcccgcatt gcgctgggtg
     2821 cctatcaagc catctccctg tctgatgcgc gacagcaacg tgaaggtatc cgtaaaatgc
     2881 tggcgctgaa tatcaacccg gtacagcagc gggctgctga acgtggctca cgaacaccgg
     2941 agaaagtttt taaaaacttg gcgctggcgt ggcataaaag taacaggaaa tggtcgcaga
     3001 acaccgccga ccgtctgctt gccagcctga acaatcacat ctttccggtc atcgggaacc
     3061 tacctgtatc agaacttaaa ccccgtcatt tcattgacct gctgaaaggg atcgaggaaa
     3121 aaggtctgct ggaggttgcg tcccgcacac ggcagcacct gagtaacata atgcgccatg
     3181 cggtccatca ggagttaatc gatacgaacc ctgcagcaaa ccttggcggc gtgaccacac
     3241 ctcctgtcag acggcactat cctgccctgc cgctggagcg gctgcctgaa ctgcttgaac
     3301 gtattggggc atatcatcag ggccgtgaac tgacccggca tgccgttctg ctgatgctgc
     3361 atgtgttcat tcgctccagt gaactgcgtt tcgcccgctg gtcagagatt gatttcacaa
     3421 accgagtctg gacgataccc gcgacgcgag aacccattat tggcgtgcgt tattccggcc
     3481 gcggggcaaa aatgcgaatg ccgcatatcg tccccctctc agaacagtcc atcgccattc
     3541 tgaaacagat taaggatatc accggtaata atgaactgat cttccccggc gaccataacc
     3601 cgtataagcc aatgtgtgaa aacacggtca ataaggcact gcgggtgatg ggttacgaca
     3661 cgaaaaagga tatctgcggt cacggcttcc gggcaatggc atgcagtgcg ctgatggaat
     3721 cgggtttatg ggcaaaggac gcagtagaac gccagatgag tcatcaggag cgcaataccg
     3781 tgcgcatggc ttatattcat aaggcagagc acctagaagc ccgcaaagcg attatgcagt
     3841 ggtggtcgga ttatctggaa gcatgccgag aatcttatgc accgccttat acaattggta
     3901 aaaataagtt tatcccatag tatactaacg aatccctcac tctggcaaat taccaccacc
     3961 tccacaggag gtggttctat atagactttt atcaacttca tgacgaaaaa cacctatttt
     4021 atcccaaaag aagacaatag agtcacagag gaatcaaaga gcgactcaat tgtctttgga
     4081 cagtctggtt gtgaggttag aaaacagtta ctcctacacc attaaatagg gcgcaatgct
     4141 cgctaatttc tcccgggttt ctgtcagttc gcgttccggc gtagattgcg cgatgattcc
     4201 cgcccccgcc tgtatccagc tgcgctggct atcctgaaat acggaacgca gaactagcgc
     4261 cgcatcaaag cgcgtatcgt ccagcagcaa gattgcgccg gaataaagct ctcgcggcgt
     4321 cttctcaatt tgcataatcg cgttcagagc agcattttta gggatacctg aggcggtaat
     4381 cgacggaaac agcacggtaa acgcatccca ggcatccttg ttttccgaaa gctgaccgct
     4441 cacgccagac cccagatgct gaacgctgcc gcgctggcga accgacatta aatcttcaac
     4501 caccacgctg cccggctggc aaacagcctc cagttcagca atagcttctt tgacagaaag
     4561 gatatgctca agcacctctt tactgtcgtg cagcagttct gcctctttcg ccttattatg
     4621 ctccgggttt cccatgcgat cgcgggtgcc cgcaagcggt tcagtgacca ctttattgcc
     4681 cgtcactgac atcaccagtt ccgggctaaa gcccagcgct tcgcgtcctt cctgacggaa
     4741 cataaacgag cgcacaggcg tgtttgcctg ccgcccgtat aacagcgtgg cgggcatatc
     4801 aatccgcgat ggcaggggaa tggcgcgcga gacaatcact ttgacatact cgccacggcg
     4861 gatctccgca acggcgcgcg caacctgttg tttatatgcc tcaccattga gggcggtatc
     4921 caccgccagt ggtgcgttct gcgtcgttgt accggcctct tttacccact cgcacaaacg
     4981 tcggcacccg tcggcggagt ccgcataaac ggtgacattt cccttttcaa aaataagttc
     5041 ttcacgggga acggttaacg tcagcagcgg ccactccccg gcgttaaagg caatcccccg
     5101 cgcatgcgcc gcaaagttga acccaacctg tccatatacc cgacgtcctt tcacctgagg
     5161 atgcgccata aaacgacgcg cgcagtcggc aatggaatcc accgcccatt tttgctcccc
     5221 ggcatcatca ataaatgtcg cctgcgttcc gtcggcatca atgtgcagcc gtgcctggca
     5281 ccctttgcct aaataccaac agggttgacg ctcatagaca taacactctt cttctgcgaa
     5341 ctggcgtaaa acgccagaaa tcgtcggcag ccattgttcc tctggtaacg ccagatgtag
     5401 aaattcactg attttcatcc cttacctctc tgtgttattc ccggatcaat caattgcgct
     5461 tgtcatcagt tctcttgccg aatgcagccg gatataagca acccacgccg ccagcaacgt
     5521 gaatgccccg gcaaacagga agcaggccgc atagccaaaa tgttgagcaa caacgccacc
     5581 gataacgcct gccagaatgg cgattgccgc atcagtacat tgaaagaggg tgaagtcgac
     5641 accggcctgc aaaggcgatg aaagcgacat cagcgtggcg taaagacaga ccagcgcgca
     5701 ggccagcgaa atggactgga caaggaccag acactggaga atctgcagca gcagatgacc
     5761 cggcgccatc atgagagtca tcaccaccgc cagcagcgcg atcccctgga cgccataagc
     5821 cgtcaacagc gctctgccgg gtgaggtgta tttcatcagc aatccgccgg ccagcgttcc
     5881 tgctatgccc gctgcaatat tgccgccgct gaacagcgcg cccaatgcgg acatgctcaa
     5941 cccatgatcc aacaacagcg gtgccagaag aggcagcaca aaccgcatgc ctgaattcag
     6001 catcaatacc agcagtaagc ccaggcgagc ctgcttcctc cttagcgcat aacctaacgc
     6061 cggaacatgc gggatagtcg ctgttcgcgt cggctccgta atgcgccaca gcgggagtga
     6121 cagcgccata atcagcaccg ccatcatcag catggcgaca ggccagccgg atgccgccga
     6181 cagcatcagg aacaccccgc cgccgcacat cattcccaga tagctgccgc cgacctgcac
     6241 gctgtttccc cagccgtaac ccgtgcgggt cagttgatcg acacaaaaac cgtcgctggc
     6301 gatatcaatg gtggaggcga ccgtgcctgc caccataaac agaacaaata acgcgacgat
     6361 ttgcgtatcg gggaatcccc cttctcgccc aaaccagcca atcgctgcga caatagtcag
     6421 gatcgccgct agcgcgacct gaccacgcag gattaacatg cgggaacggc gttcctggct
     6481 accgggcgga agccgccagc gctcgatcca cggcgcccaa ataaacttca gcgcccaggg
     6541 cagcataaac agggttgtcg caccggcaag cgccagcgaa ccgccagcag cgcgcaccag
     6601 cgcgggtaag gactgcatag acagcgccga taccagactc tgcgtcacat agacgccggc
     6661 aatagcaaaa atcacccgcc cggtcgtcaa cgtcagcggt ttcacattcg actgaacatc
     6721 actcatgcac ccactctcca gacgcggaaa ccccgtcgtc gcgccacacg cgcgcggcca
     6781 tttgcgcctg ccacagcgcc tgataacgtc catgatgtga gagcaattgc gcatgagtac
     6841 cctgctcaac cacctgtccc tcttccatca ccagaatgtt tccggccccg gcgatggtgg
     6901 ataaacggtg agcgatgata atcaccgtcc ggttgtgaac caggttatcg atcgcttttt
     6961 gcaccgccag ctcgctttca atatccagcg cggcagtcgg ttcatcgaga atgacgaccg
     7021 gcgcgttttt caataacgct ctggcaatgg aaatccgctg gcgctcgccg cccgatagct
     7081 ggccgcccat ctctcccatt ggcgtcagcc agccttgcgg aagacgggaa ataaactcaa
     7141 ggcactgcgc cgcgcgggcg gcctcttcta cctcctgccg cgtcgcctgt gggcgcgcga
     7201 tacggatatt cgccagcagc gtgtcatcaa acagccagac atcctggaaa acaacagaaa
     7261 tcaggctatt gagctgttcc ggcgtcaggc ggcgaatatc gacgccgcca atagagatct
     7321 gcccttgctg cggatcggca tagcgcatta acagtttggt gaccgtagtt ttgcctgcgc
     7381 cggaggcacc caccagcgcg ctcatactgg ctgccgggaa cgtcaaagaa acatgattaa
     7441 gcgcgtagcc gtcgccttct tcgtagcgat agctgacgtt gtcaaagcgg atatcgtaac
     7501 gttccggcat ctcgctttgc tctgcgacgg gaagcggtgc tatcgccata aactgctcaa
     7561 tccgttgcag ggcgctggcg atcagttcca caaccgaggt gtagctgata aacatcgcca
     7621 tcggttctgc gaagcgcata atcatcgcga cggcggcaat caaaaaggcg aggttcagag
     7681 tgcccgtcac tacccagacg atcccggata acaccaccac ctgtaggccc aactccacga
     7741 cgctggcgat cagcatcgtt gcgccagcgc cctgacggtg agtgcgggtc tgtaagtttt
     7801 ccagcgcatt gaaatgcgcc agcagcgccc ggcttttatc ggcatcgctg ccgcaggtgc
     7861 gcaataccat catcccctga gcaaattcaa cgatatcgcc gctcagacgc tggtgcgctt
     7921 cccccagcgt ctgcatttgt cgccgcatcg ccgggcggcg ccagtaataa aacggcacca
     7981 gcagagggaa gatcagcaac atcaccagcc ccagccgcca gtctatccac aatgtcgcca
     8041 gcgacgccgt cagcggcgtg acaatggtga gcagcaaaat attggctatc gcaataacat
     8101 aattgaggtt ttcatccacg ctgcccagca gcaaggcgtt catttcaccc gccctgccgc
     8161 gctggagctt ctccagcggc acgcggcgta actgctcgcc aagtcgcagg cgcaactcat
     8221 gggtagcctg cgccagatga ccacggtatt caaagcccag gccataccag cgtagcacca
     8281 gcgtcacaat tgcggcgacg ctgaaggcca tagcccagtt aagaagttgc ggcgcgtctc
     8341 cccgtaacag cgcatcaatg atcggataaa gacaggcaaa ggcgatcccc tgcatgaatg
     8401 ccgccagcaa cagcgccagc atactgcgtc gcagcatcct ggcctgactg ccaacgctgg
     8461 agataagctg gcgccagatg acgcgccagg ccaggttatc cgcaggatta ttgtctttca
     8521 ttttccacca cctcttcctg tgttccaccc ggcacccaat gctgcgcctg ctgatagtgt
     8581 tgccagagcc gttgatacag gccgccctgc gccaacagtt gcgtatggtt ccccatttcc
     8641 ctgagctgtc cgtcggaaaa cagcaatatc acatcggcct gagtcaccat tgagagacga
     8701 tgcgcgacca tgatgaccgt ccggccacgc atggccgccg cgagcgcctt gataagcgcc
     8761 gcttcgtttt ccgggtcagc aaacgccgtc gcctcatcga gcaccaggat ggggcgatcc
     8821 tgcaaaagcg cccgggcgat agtaatgcgc tgccgctggc cgccggagag aaatacccca
     8881 cgttccccga ctcgagtgtt gtaaccttct ggcagagcgc taataaaatc atgggcctgc
     8941 gccactctgg ccgccgctat aaccgcctcc agcggcgtat ccggcgcgcc cagacgaatg
     9001 ttattggcta tcgtgtcggc aaaaaggaag ttgtcctgaa acacaaacga gagttgcttc
     9061 atcagggtgt ccgtctgcat atcacgcaga tccacgccgc caatacggat atggcctttg
     9121 tccgggtcgg cgtaacgcag cagcaagcgc gccacggtgc tttttccggc gccgcttggc
     9181 ccgaccagcg ccacaatttg cccggcaggc acatgaaagc tcacctcctg caacgcggcg
     9241 ccagtacgcg cttgcggata atgaaagctc acctgctcaa aggtaatgct cgcctcttgc
     9301 ggctgctggt cagactgcgg cagcgataac tccggcatcg cgagaacctg ataaatacgc
     9361 tgaatgctta aacgcgtttg cgcgaccagg ttattgagca tcatcattgg cattacggct
     9421 tcggccattc cgctgcccag cagtaaaacg gccacccacg cgataaaatc gaaactgcca
     9481 tagtgcaaca ggccgtatcc cgaccagatc aggacaaaca gggtcgggag aggattcaga
     9541 atcgagaagg aaaaacgcgc tgaaaaaccg gctttgcgat accaggtttt gagcacatcg
     9601 acccactctt caagggcgcg ttgatagcgc aaaaaactgg tgctgccgct atcaaacgtt
     9661 ctcaccacgg gcatggcctg cacgaactca ataaccgccg cgctaacctg ctcgcgcgcc
     9721 ttatgataac gctgagccat attttccgaa cgctggcggg cgagcacgag aataaccgat
     9781 ccaaacgcca gtacccccag cgtcgcgatt gccagccgcc agtccagcca gaacaagata
     9841 acgattgtcg ccagcggcat gatgatcgcg cgggcataga gcggcgtgct gtcggccaca
     9901 aaaatatgta acgacttcac gtcatccagc atcactttcg ccactgaccc gctgcccatt
     9961 tgctgtaaca cgccaggcgg aagctgcaat gctttacggg ctaacctgct gcgcagaatg
    10021 ttctccagat gaaaagccgc gtaatgagag gtgttgaacg cctgtaagcg cagaacaaaa
    10081 gttaagacca cgcagccgat cacgccgccc agcggccagg ccggaatagc gtcaggcgtc
    10141 gcgcgaatgt cacgcaggct ccacgccaga aaaaggagcg cccccaggct ggttaatgcc
    10201 gccagccccg ccagcgccat agctaaaata accttcctgc gaaccggtgt aatcacctgc
    10261 cagagaggaa atcgggacag cgattcagta ttggatgatt gagatgacat gggagtaact
    10321 gaatttcctg atgaattttt acggacgcca aaaacgacaa ttcagggtgg cgttctgaga
    10381 attaatgtga ataataacca ttatcaataa ctcatctacc ccattcggtt ctatttatac
    10441 ccgcattggt ctaagccaca gggagataac caggtcatga cggagtcacc gcaaacgcaa
    10501 tctgaaatct ctattcacca gttggtggtc gggaaacctg caaacgatgg caatattcct
    10561 gctcagtgtg aactgctgcg ctgttcgttg caggaaggta tggatatttt gctctggagg
    10621 ggccattttg cgcggccaga gacgttgcag ctacacgacg atctgggcag gattaacttc
    10681 tcgtgcatcc tggaaggcac atcgcgcttt gcgattcagg gactccggcg ccacaccgac
    10741 tgggagcttg cgcgcaatcg ccattacatt acccatacgc cagactgtcg cggcagcgcc
    10801 tcctattgtg gccgattcga gagcattacc ctctccttca gccctgaaac cctcgcgctg
    10861 tgggtgccgg atattagcgc ggtcattaag aataagatcg actcccactg ctgttgccag
    10921 cagcagcgtt gtaatgctga aacccactta acggctcagg ccttgcgtca cgcgctgatg
    10981 cgcatgcacg gcggcttcag tcatgaacaa aagccgtcga cgctatggtt gcaggggcaa
    11041 agtctggtca tgctcagtct ggtgctggat gaacatcgcg aagacgccag ttgcctctcc
    11101 tgccacttca acccgatgga acgccagaaa ctgctgcggg cgaaggatct gctgctggct
    11161 gatctgacgc aagcgccggg cgtcgcggag ctggccagag agtccggtct gagcgttctc
    11221 aaaattaagc gaggcttccg cgttctgttc aacaacagcg tctatggcct gtttcaggct
    11281 gaacgaatgc aggaagccag aagacgcctg gccaacggca atacctccgt catgacggtg
    11341 gcggccgatc tgggctatgc caacgccagc catttctctg ccgccttcca gaaacagttt
    11401 ggcgtaactc cttcgacctt taaacgcggg atgtaaccgg ggtcgcgccc ccctaaactc
    11461 ctccctgaca gaggcccgga taccccatcc gggcctctgt tctttccgcc tgttaccaac
    11521 ccgaaacggg cataaataaa cccgtttcgg gtagcattca attgttaata attattattc
    11581 tcatatgagt aatgcttttc ggtaagacgt gccatcagga ggaagaatga tttctggcgc
    11641 accatctcag gattcgctgt taccggacaa ccgccacgcg gctgattacc aacaattacg
    11701 cgagcggctc atacaggaac tgaatttaac gccgcagcag ttacatgaag agagcaacct
    11761 gatccaggcc ggcctggatt ccataagatt gatgagatgg ttacactggt ttcgtaaaaa
    11821 tggctaccgc cttacccttc gcgagctgta tgccgccccc acgctggcgg catggaacca
    11881 gttaatgctc agccggtcgc cggagaacgc ggaagaagaa acgccgcccg acgaatcatc
    11941 ctggccgaac atgaccgaaa gtaccccctt cccattgacg ccagtacagc acgcctacct
    12001 gacgggccgc atgccggggc agacgcttgg cggcgtgggt tgccacctgt atcaggagtt
    12061 tgaaggccat tgtctgacgg cgtcgcagct ggagcaggcc atcacgacct tgctgcaacg
    12121 ccacccaatg ctgcatatcg cctttcgccc cgacgggcag caggtctggc taccgcaacc
    12181 ttactggaac ggcgtcaccg ttcatgattt acgccataac gacgctgaaa gccgccaggc
    12241 ctatctggac gcactgcgcc agcgcctgag ccaccgtctt ttacgcgtgg aaatcggcga
    12301 aacgtttgat tttcagctga cgctcttgcc ggacaatcgc caccgcctcc atgtcaatat
    12361 tgacctgctg attatggatg cctccagctt tacgcttttc ttcgatgagc ttaacgccct
    12421 gctggccgga gaatcgctgc cggctatcga cacccgctat gatttccgct cgtatttgct
    12481 gcaccagcag aagatcaatc aaccactgag agacgtcgcg cgcgcttact ggctggcgaa
    12541 agcatcgacg cttccccccg cgcccgtctt gccgctggcc tgcgaacccg ccacgctacg
    12601 tgaagtccgt aatacccgac gccgcatgat tgtcccggca acacgctggc acgcctttag
    12661 caaccgggcc ggcgagtatg gcgtgacgcc gacaatggca ctggcgacct gtttttctgc
    12721 cgtgctggct cgctggggcg gcctgacgcg tctgctgctt aacatcacct tattcgaccg
    12781 ccagccgctg caaccggcgg ttggcgcgat gcttgccgac ttcaccaata ttcttctgct
    12841 ggataccgcc tgcgatggcg ataccgtcag caacctggcg cgtaaaaacc agctcacgtt
    12901 tacggaggac tgggagcatc gccactggtc cggcgtcgaa ttactccgtg aactcaaacg
    12961 ccagcagcgc tacccccacg gcgccccggt ggtatttacc agcaatctgg ggcgttccct
    13021 ctacagcagc cgcgcagaat cgccgttggg cgagccggaa tggggcatct cgcaaacgcc
    13081 gcaggtctgg atagatcatc tggcgttcga gcatcacggc gaggtctggc tacaatggga
    13141 cagcaacgac gcgctgttcc ctccggcgtt agtcgaaaca ttgttcgacg cctactgcca
    13201 gttgattaac caactctgcg atgacgaaag cgcctggcaa aagccgttcg cagatatgat
    13261 gcccgccagc cagcgcgcga tacgcgaacg ggtcaacgcc accggcgccc ccattcccga
    13321 aggcttgctg catgaaggca ttttccgtat cgctctgcaa cagccgcagg cgctggcggt
    13381 aacggacatg cgttatcagt ggaattatca tgagctgaca gactatgccc gccgttgcgc
    13441 gggcaggtta atcgagtgcg gggttcagcc cggcgataat gtggctatca cgatgtcgaa
    13501 aggcgcagga caacttgttg cggttctggc cgtcctgctg gccggggcgg tttacgttcc
    13561 ggtttcgctg gatcagcctg ccgcacggcg cgagaaaatc tacgctgacg ccagcgtccg
    13621 gctggtgctc atttgtcagc acgacgccag cgccgggtca gacgatattc ccgcccttgc
    13681 ctggcagcag gccattgagg cggagccgat cgccaacccg gtagtacgcg cccccacgca
    13741 accggcctac attatctaca cctccggctc taccggtacg ccgaaagggg tagtcatttc
    13801 tcaccgggga gcgcttaaca cctgttgcga tatcaatacc cgctatcagg ttggcccgca
    13861 tgacagggtg ctggccctct ccgccctaca ttttgattta tcggtttacg acatttttgg
    13921 cgtactgcgc gcgggcggcg cgctggtgat ggtgatggaa aatcaacggc gcgatcctca
    13981 cgcatggtgt gagctgatcc agcgccatca ggtcacgctc tggaacagcg tcccggcgct
    14041 gttcgatatg ctgctgacct ggtgtgaagg tttcgccgac gccacgccgg aaaacctgcg
    14101 cgcagtgatg ctttccggcg actggatcgg gcttgacctc cccgcccgtt atcgggcctt
    14161 ccggccacaa ggacaattta tcgcgatggg cggcgccacc gaggcgtcta tctggtctaa
    14221 cgcctgcgaa attcacgacg tccccgccca ctggcgctcc atcccttacg gttttccgct
    14281 aaccaaccaa cgctaccggg tggtggatga acagggccgg gactgccctg actgggtgcc
    14341 gggtgaatta tggattggcg gcattggggt cgcggaaggc tatttcaacg atcccctgcg
    14401 tagcgagcag caatttttga cgctcccgga cgagcgctgg tatcgcaccg gcgatctcgg
    14461 ctgctactgg ccagatggca caatcgagtt cctcggtcgt cgcgacaagc aggtcaaagt
    14521 cggaggatat cgcatcgagc tgggcgaaat cgaaagcgcg ctcagccagc tggtgggggt
    14581 gaaacaagca accgttctgg cgatcggcga aaaagaaaaa acgctggcgg catacgttgt
    14641 tcctcagggc gaggcttttt gcgttaccga tcatcggaac ccggtactgc cgcaggcgtg
    14701 gcacacgctt gcgggaacgt tgccctgttg cgccatctcg ccagagatct ccgcagaaca
    14761 ggtagccgat ttccttcagc atcgcctgct aaaactgaag ccgggtcaca ccgctggcgc
    14821 cgatcctctc cccctgatga actcactcgc tatccagccg cgctggcagg ccgtggtgga
    14881 acgctggtta gcatttctgg tgacacaacg gcgactgaag cccgctgctg aaggttatca
    14941 ggtctgcgct ggtgaagaac gcgaggatga gcacccgcac ttcagcggac atgatttaac
    15001 gttatcgcaa attcttcgcg gtgcccgtaa cgaactgtcg ttactgaacg acgcgcagtg
    15061 gtcgccggaa agcctggcct ttaaccatcc ggccagcgcc ccgtatattc aggaactggc
    15121 gacaatttgc caacagcttg cacagcgctt acagcgcccg gtacgcctgc ttgaggtggg
    15181 aacccgcact ggccgcgccg cagaatcgct gttagcacag ctcaacgccg gacagattga
    15241 gtatgtcggg cttgagcaga gccaggagat gctgctgagc gcccggcaga ggctcgcccc
    15301 ctggcctggc gcccgtctgt ccctctggaa tgcagacacg ctggcggcgc acgctcactc
    15361 ggcggacatt atctggctta ataacgccct gcatcgtctg ctgccggaag atcccgggct
    15421 ccttgcgaca ttacaacagc ttgccgttcc cggcgcgctg ctctacgtga tggagtttcg
    15481 ccagttaacg ccgtccgccc tactcagcac gctcctgtta accaatgggc agccggaggc
    15541 cttgctgcat aacagcgccg actgggcggc attatttagc gcggccggct tcaactgtca
    15601 gcatggcgat gaggtcgcgg ggttacaacg cttcctcgta caatgtcctg acaggcaggt
    15661 gcgccgcgat ccccgtcaac ttcaggccgc cctcgccggg cgtctgccgg ggtggatggt
    15721 gccgcaacgg atcgtcttcc tcgacgcctt accgctgaca gctaacggga aaattgacta
    15781 ccaggcgctg aagcgtcgtc atacccctga agcggaaaac ccggccgaag cggatttacc
    15841 ccagggcgac attgaaaaac aggttgccgc cctctggcag caactcttat caactgacaa
    15901 tgtcaccaga gaaaccgact tcttccagca aggcggcgat agcctgctgg cgacccgtct
    15961 gaccgggcaa cttcatcagg caggttatga agcgcaatta agcgacctgt ttaatcatcc
    16021 ccggctggcg gattttgccg ccacgctgcg gaaaaccgac gtcccggtcg aacaaccatt
    16081 cgtccactcc cttgaagatc gctaccagcc ctttgcgctt accgacgtgc agcaggctta
    16141 cctggtgggg cgtcagccgg gctttgccct gggcggcgtc ggctcacatt tatttgttga
    16201 atttgaaatt gccgatctgg acctcacccg gctggagacg gtctggaacc gattaatcgc
    16261 ccgccacgat atgctgcgcg ccatcgtgcg tgatggacag caacaggtgc tcgaacagac
    16321 gcccccctgg gtgatacccg cacacaccct ccatacgcct gaagaggcgt tgcgggtgcg
    16381 cgaaaaactg gcgcatcagg tactcaaccc cgaagtgtgg ccggtattcg atctccaggt
    16441 cggatacgtg gacgggatgc ctgcccgcct gtggctgtgt ctggataacc tgttgcttga
    16501 cggtctgagc atgcagatcc tgctggcgga gctggagcac ggctaccgct acccgcaaca
    16561 gctgcttccg ccgctgcccg tcaccttcag ggattatctg caacaaccct cgctacagtc
    16621 gcccaatcca gattctctgg catggtggca ggcgcagctt gatgatattc ctccggcgcc
    16681 tgcgttgccg ctgcgctgct tgcctcagga ggttgaaaca ccgcgcttcg cccgcctgaa
    16741 cggcgcactg gacagcacgc gctggcatcg gctgaaaaaa cgggcggctg acgcccatct
    16801 caccccgtcg gccgtactgt tgtcggtgtg gtcaacggtt ctctctgcat ggagtgcaca
    16861 gcctgacttc acgcttaacc ttacgctttt cgacaggcga ccgctgcacc cgcaaatcaa
    16921 ccagattctg ggcgatttca cctcgctgat gctgctgagc tggcatcccg gcgaaagctg
    16981 gctgcacagc gcgcagtcac tacagcagcg gctgagccag aacctcaacc accgcgatgt
    17041 gtcagccatc cgcgtgatgc gtcaactggc gcaacggcaa aacgtgccag ccgttccgat
    17101 gcccgtcgtc tttaccagcg cgctgggctt tgagcaggat aacttcctcg cccggcgtaa
    17161 tctgctcaaa ccggtctggg gcatctccca gacgccgcag gtctggctcg atcaccagat
    17221 ttatgaatcc gaaggcgaac tgcgctttaa ctgggatttt gtcgccgcgc tgtttcctgc
    17281 cgggcaggtg gagcgccagt ttgaacagta ttgcgcattg ctaaaccgaa tggccgagga
    17341 tgaaagcggc tggcaactgc cgctcgccgc gctggtgcct cccgttaaac acgcagggca
    17401 atgcgcagag cgctcaccgc gcgtatgccc tgagcactct cagccacaca ttgcggcgga
    17461 cgagagcacc gtcagcctga tttgcgacgc cttccgcgag gtggttggcg agtctgtcac
    17521 gcccgcagaa aacttctttg aggcgggcgc aacgtcgctg aatctggtgc aactgcacgt
    17581 tttgttacaa cgtcacgaat tttccaccct gacgttgctt gacctcttca cccacccttc
    17641 tcctgctgcc ctggccgatt atctggccgg cgtcgccacg gtggagaaaa cacaacgacc
    17701 tcgccctgtt cgccgtcgtc agcggcggat atagcgcgaa gcaaactgat tttccccgga
    17761 acgccatcgc gaacgcatgg cgttccattg actttgtgaa tcttaggaaa cgggaccgat
    17821 tatggataac ttgcgcttct cttctgcgcc gacagcagat tccattgatg catcgatcgc
    17881 tcaacactac ccggactgcg aacctgtcgc ggttatcggc tacgcctgcc attttcctga
    17941 atcgccggat ggcgaaacgt tctggcaaaa tctgctggaa ggtcgtgaat gcagccgacg
    18001 ctttacgcgc gaagagcttc tggccgtcgg tctggatgcc gccatcattg acgatcctca
    18061 ttatgtcaat atcggtacgg tgttagacaa cgccgactgc ttcgacgcca ccctgtttgg
    18121 ctattcgcga caggaagcgg agtcgatgga cccgcagcag cgcctgtttt tgcaggcggt
    18181 ctggcatgcg ctggaacatg ccggttatgc ccccggcgcc gtcccccata agaccggcgt
    18241 tttcgcctct tcccggatga gtacctaccc cggtcgcgaa gcattgaacg tgacagaagt
    18301 cgcgcaggta aaaggtctgc aatctctgat gggcaatgat aaagactata ttgccacccg
    18361 cgccgcgtac aaactcaacc tgcacggccc ggcgttatcg gtacagaccg cctgctccag
    18421 ctcgctggtt gccgtgcatc tggcctgtga aagcctgcgc gctggcgaat ccgatatggc
    18481 ggttgccggc ggcgtggcgc tctctttccc ccagcaggca ggctaccgct accagcccgg
    18541 aatgattttc tctcctgatg gtcactgtcg tccctttgac gcctcggctg agggcacctg
    18601 ggccggtaac ggtctcggct gcgtggtgct gcgtcgcctg agagacgcgc tgctgtcagg
    18661 cgatccgatt atctcggtga tcctctccag cgcggtcaac aacgacggca acagaaaggt
    18721 cggctatacc gccccttccg tcgcagggca acaggcagtc atcgaagagg cgttaatgct
    18781 ggcggccatc gacgacaggc aggtaggtta cattgaaacc cacggcaccg gcacaccgct
    18841 gggcgacgcg attgaaattg aagcgttacg caacgtctat gcgcctcgcc cgcaggatca
    18901 gcgctgtgcg ctcggttccg tgaaaagtaa catgggccat ctggataccg cggcgggcat
    18961 tgccggactg ctgaaaaccg ttctggcagt cagtcgcggg caaattcctc ccttactgaa
    19021 ttttcacacc cccaacccgg cgctgaaact tgaagagagc ccctttacca taccggtgtc
    19081 ggcacaggca tggcaggacg aaatgcgcta tgcgggcgtc tcctcctttg gtattggcgg
    19141 caccaactgc catatgatcg tcgcctcgct gcccgacgcg ctcaacgcgc gcctccccaa
    19201 tacggatagc ggcagaaaaa gtaccgcgct gctgctcagc gccgccagcg acagcgcgtt
    19261 gcggcggctg gcgacggatt atgccggggc gctgagagag aatgcggatg ccagctctct
    19321 ggccttcaca gccctgcacg cgcgccgtct cgatctcccc ttccgcctgg cggcgccatt
    19381 aaaccgtgaa accgccgagg cgctcagcgc ctgggctagt gagaaatcgg gggcgctggt
    19441 ttacagcggc cacggcgcca gcggcaagca ggtgtggctg tttaccggcc agggctcgca
    19501 ctggcgcact atgggtcaaa cgatgtacca gcactcaacg gcgtttgccg acacgctgga
    19561 tcgctgtttt tccgcctgta gcgaaatgct cacgccgtca ctgcgcgaag cgatgtttaa
    19621 ccccgattcg gcgcagctgg acaatatggc ctgggcgcag ccggcgattg tcgcgtttga
    19681 aatcgcgatg gcggcgcact ggcgtgctga aggactgaag ccagacttcg ccattgggca
    19741 ttccgtcggt gaatttgccg ctgccgttgt ctgcggacac tatacgattg aacaggtcat
    19801 gccactggtt tgtcggcgcg gcgcgctaat gcagcagtgc gcaagcggcg cgatggtggc
    19861 ggtatttgca gacgaagaca cgctgatgcc gctggctcgc cagtttgagc tggatctcgc
    19921 cgccaacaac ggtacgcaac atacggtatt ttccgggccg gaagcccgtc tcgcggtatt
    19981 ttgcgccacg ctctcgcagc atgacattaa ctatcgtcgc ctgagcgtaa ccggtgcggc
    20041 gcactccgct ttactggagc cgatactcga tcggttccag gacgcctgcg cgggactgca
    20101 cgcggagccg gggcaaatac cgattatttc cacgctcacc gccaacgtca ttgatgagtc
    20161 aacgctcaac caggcggatt actggcgccg acacatgcgc cagccggtgc gttttatcca
    20221 gagtattcag gtggcgcatc agctcggcac ccgcgttttt ctggagatgg ggcccgatgc
    20281 ccagttggtt gcttgcgggc agcgcgaata ccgcgataac gcatactgga tagccagcgc
    20341 ccggcgtaac aaagaggcga gcgatgtcct caatcaggcc ctgctccagc tttacgctgc
    20401 cggcgtcgcc ctaccgtggg ccgacctgct ggcgggcgat ggacaacgta tcgctgcgcc
    20461 atgttatccg tttgatactg aacgttactg gaaagagcgc gtctccccgg cctgcgagcc
    20521 tgccgacgca gcgctgtctg ccgggctgga ggtggcgagt cgcgccgcga cagcgctcga
    20581 tctccctcgc ctggaagcgc ttaaacagtg cgccacgcga ctgcacgcca tctacgtcga
    20641 tcaactggta caacgctgta ccggcgatgc catagagaac ggcgtggacg ccatgaccat
    20701 catgcgccgt ggacgtctgc tgccccgcta ccagcagcta ctccagcgcc tgctgaataa
    20761 ctgcgtggtc gacggcgatt accgctgcac cgacgggcga tacgtccgcg cccgccccat
    20821 tgaacatcaa cagcgggaat cactgctgac ggaacttgcc ggttattgtg aaggttttca
    20881 ggctattccc gacaccatcg cccgtgccgg cgatcggtta tatgaaatga tgagcggcgc
    20941 ggaagaaccg gtggcgatta tcttcccgca aagcgcctcc gacggcgtgg aagtgctgta
    21001 tcaggaattc agctttggcc gctatttcaa ccaaatcgcc gccggggtat tacgcggcat
    21061 tgtccagacg cgtcagcccc gccagccgtt gcgtattctt gaagttggcg gcggaaccgg
    21121 cggcaccacc gcgtggctgc tgccggaact caacggcgtt ccggcactgg agtaccattt
    21181 caccgatatc tcggcgctgt tcacccgtcg cgcccagcag aaattcgccg actatgattt
    21241 tgtgaagtat agcgagctgg atctcgaaaa agaggcgcag tctcagggtt tccaggcaca
    21301 gtcttacgat cttatcgtgg cagcgaacgt gattcacgcc acccgccata ttggccgcac
    21361 gctcgataat ctgcgccccc tgctcaagcc gggcgggcgc ctgctgatgc gcgaaatcac
    21421 ccagccaatg cgtctgtttg acttcgtttt cggcccgctg gttcttccgc tacaggatct
    21481 cgacgcccgc gaaggtgagt tattcctcac caccgctcag tggcaacaac agtgccgcca
    21541 cgccggattc agcaaagtgg cgtggctacc gcaggatggc agcccgaccg ccgggatgag
    21601 cgaacatatc attctcgcca cgctgcccgg tcaggcggtt agcgccgtaa cattcaccgc
    21661 gccatcagaa cccgtgttgg ggcaggcgct gacggataac ggtgattatc tcgccgactg
    21721 gtctgattgc gcaggtcagc ccgaacagtt taacgcccgc tggcaggagg cctggcgtct
    21781 gctttcacag cgtcatggcg acgctctgcc tgtggaaccg ccccccgtcg ccgccccgga
    21841 gtggctgggg aaggttcgct taagctggca aaacaaagcc ttttcccgcg gtcagatgcg
    21901 cgttgaagcc cgtcatcctg ctggcgagtg gctgccgcta tcgcccgccg cgcctcttcc
    21961 tgcgccgcaa acgcattatc aatggcgctg gacgcccctc aacgtcgcca gcattgacca
    22021 tccgcttacc tttagcttca gcgccggtac gcttgcgcgc agcgacgagc tggcgcaata
    22081 cggcatcatt cacgatccgc acgcctcttc acgactgatg attgttgagg agagcgagga
    22141 tacgctggcc ttagcggaga aagtgatagc agcgctcacc gccagcgcag ccggattgat
    22201 tgtggttact cgccgcgcgt ggcgagtcga ggaaaatgaa gcactctctg catcccatca
    22261 cgcgctatgg gccttgcttc gcgtcgcggc caacgaacag ccggaacggt tgcttgccgc
    22321 catcgatctc gccgaaaaca ccccgtggga aacgctgcat caagggttga gcgcagtctc
    22381 actatcacag cgctggctcg ccgcacgggg tgacaccctt tggctccctt cactatcgcc
    22441 caatacggga tgcgccgctg aattaccggc aaacgtgttt accggcgata gccgctggca
    22501 tctggtgacc ggagcgtttg gcggattagg ccgccttgcc gtgaattggc tcagagaaaa
    22561 aggggcgcga cgcatcgccc tgctggcgcc gcgcgtggat gagtcatggc tacgcgacgt
    22621 ggagggcggg cagacgcgcg tctgccgttg tgatgtgggc gatgccgggc aactggccac
    22681 ggttcttgac gatctggcgg ccaacggcgg cattgccgga gcgattcatg ccgctggcgt
    22741 attggctgac gcgcccttgc aggagcttga tgaccaccag ctggctgccg ttttcgcggt
    22801 aaaagcgcag gcggcaagcc agctgttgca aaccctgcgc aaccacgacg gacgctatct
    22861 tattctctac tcttccgctg ccgccaccct cggcgcgccg ggtcagagcg cccatgcgct
    22921 ggcctgcggc tacctggacg ggctggccca gcagttttcc acccttgatg cgccgaaaac
    22981 gctctctgtc gcctggggcg catggggaga aagcggtcgg gcggccacgc cggaaatgct
    23041 ggcgacgctc gccagccgag gtatgggcgc gttaagcgat gccgaaggct gctggcacct
    23101 ggaacaggcg gtgatgcgcg gcgccccgtg gcgactggcg atgcgcgttt ttaccgacaa
    23161 aatgcccccg ttacaacagg ctctgtttaa catcagcgcc acagaaaaag ccgcaacgcc
    23221 ggtcattcct cctgctgatg acaacgcctt taacggcagc ctgagcgatg aaacagcggt
    23281 gatggcatgg ctgaaaaagc ggattgcggt tcagctaagg ctgagcgatc cggcgtcact
    23341 gcatccaaac caggatctgt tgcaactcgg catggactcg ctgctcttcc ttgaactcag
    23401 tagcgatatt cagcactacc tgggcgtacg catcaatgcg gaacgggcgt ggcaggatct
    23461 gtctcctcat ggactcacgc agcttatctg ttctaagcca gaggcgacgc ctgccgcttc
    23521 gcagccggaa gtgttgcggc acgacgccga cgagcgttat gcgcccttcc ctttgacgcc
    23581 cattcagcac gcctactggc tggggcgaac ccacctcatt ggctatggcg gcgtcgcctg
    23641 tcacgtcctg tttgagtggg ataaacgcca cgatgagttc gatctcgcca tactggagaa
    23701 agcatggaac cagctcatcg cacgccacga tatgttgcgt atggtggttg atgccgccgg
    23761 gagaccctcg cgctggacgc cagcagaaac cgagtggcag ggctggataa acaacgccga
    23821 cgacgctgtg attgaagcca gccactggca aatcatgatg gaagcccccc acgttcaggc
    23881 ttgtgcgcaa cacattacgc gctggctttg cgcaacctca acgcaaccgg agaacacgtt
    23941 atgatgccgt ccgcctcccc aaaacaacgc gtactgattg tgggcgccaa atttggcgaa
    24001 atgtacctga atgcctttat gcagcccccg gaggggctgg aactggtcgg cctgctggcg
    24061 cagggaagcg cccgttcaag agagctggct catgcgtttg gcattccgct gtatacctcg
    24121 ccggaacaga taaccaggat gccggatatc gcctgtattg tcgtgcgttc gaccgtcgcc
    24181 ggaggaaccg gtacgcagct tgccaaacac tttctgacgc gcggcgtaca cgtcattcag
    24241 gagcatcccc tccacccgga tgacatcagt tccttacaaa cgctggcgca ggaacagggc
    24301 tgctgctatt gggtaaacac gttttatccg catacgcgcg cgggacgcac atggcttcgc
    24361 gatgcgcagc agctgcgtcg ctgcctggct aagacgccgc cggtagtcca cgccaccacc
    24421 agccgacagt tgctctactc cactctggat ttgttgttgc tggctctggg cgttgatgcc
    24481 gccgccgtgg agtgcgacgt ggttggcagc tttagcgatt ttcactgtct gagactcttc
    24541 tggccggagg gagaagcctg cctgctgctt cagcgctatc tcgatcctga cgatccagat
    24601 atgcatagcc ttatcatgca ccgcctgctg ctgggctggc cggaaggtca tctttcgctg
    24661 gaggcgagct acggccccgt catctggtca tccagcctgt ttgtcgcaga ccatcaggag
    24721 aacgcccaca gcctctaccg cagaccggag atcctgcgcg atctgccggg tctgacgcgc
    24781 agcgcggcgc ccctgagctg gcgcgactgc tgcgaaaccg tcgggccgga gggcgtcagt
    24841 tggttgctac accagttgcg cagccatctg gcgggcgaac atccaccagc ggcctgccaa
    24901 agcgtacatc agatcgcgtt atcacgttta tggcagcaga ttttacgtaa gaccggcaat
    24961 gcggagatca gacgcctgac gccgccgcac cacgaccggt tagccggttt ctacaacgat
    25021 gatgataagg aggcgctgtg acgcaatctg caatgtgcat cccgctgtgg cccgcccgga
    25081 acggcaatac tgcgcatctg gtcatgtgcc ctttcgctgg cggcagcagt agcgcgtttc
    25141 gccactggca agctgagcaa ctggctgatt gcgcgctttc tctggtgacc tggccggggc
    25201 gcgatcgcct tcgccatctg gaaccgctca gaagcattac acaactggcg gcactgctgg
    25261 cgaacgagct ggaagcatcc gtatcgcctg acacgccgct tttactcgcc gggcacagca
    25321 tgggggcgca ggtggcgttt gaaacctgcc gacttctgga gcaacggggg cttgcgccac
    25381 aaggactgat tatttccggg tgccatgccc cgcatctgca ttctgaacgc cagctcagcc
    25441 atcgcgatga tgccgacttt atcgctgagc tgatagacat tggcggatgt tctcctgaac
    25501 tgcgggaaaa ccaggaatta atgtcgctgt ttcttcctct tctgcgcgct gatttttacg
    25561 ccaccgagag ctatcactac gactcgcccg acgtctgtcc gccgctgcgc acgcctgcgc
    25621 tgttattgtg cggcagccac gatcgcgaag cctcctggca gcaggtcgat gcctggcgtc
    25681 agtggctgag ccacgttaca ggcccggtgg tgattgacgg cgatcatttc tatcccattc
    25741 aacaagcccg gtcctttttt acgcagattg tccgccattt tccccacgca ttttctgcaa
    25801 tgaccgcgtt gcaaaaacag cccagtactt cagaaaggtg acgcatgaat tcttcctttg
    25861 aatctctgat tgaacagtat cccttaccca ttgccgaaca gttgcgccac tgggcggccc
    25921 gttatgcctc gcgaattgcc gtcgttgatg caaaggggtc gttaacctac agcgcgcttg
    25981 atgcacaagt tgacgaactt gccgcaggtc tgtcatcact gggtttgcgt tcgggggagc
    26041 atgtaattgt gcagcttccc aacgacaacg cgtttgttac cctgctgttc gccttgttaa
    26101 gactgggcgt tatccccgtg ctggcgatgc cctcgcaacg ggctctggat atcgacgcgc
    26161 tgattgagct ggcgcaaccc gtcgcttacg ttattcacgg ggaaaaccac gcagagctgg
    26221 cccgacagat ggcgcacaaa cacgcctgct tgcgtcatgt tctggtcgct ggagagaccg
    26281 tgagcgacga ttttacgccg ctcttctccc ttcacggtga gcgacaggca tggccgcagc
    26341 ctgatgtttc cgccaccgcg ttgttgttgc tctcaggcgg cacaaccggc acgcccaaac
    26401 tcatcccgcg ccgacatgcc gactatagct ataacttcag cgcttctgct gaactgtgcg
    26461 gcatcagcca acagagcgtg tatctcgccg tcctcccggt ggcgcataac tttccgctgg
    26521 cctgccccgg tattctggga acgcttgcct gcggcggaaa agtggtgctg accgacagcg
    26581 ccagctgtga tgaggtgatg cctttaatcg cgcaggaaag agtgactcac gtcgccctgg
    26641 ttccggcgct ggcgcaatta tgggtgcagg ccagggagtg ggaagacagc gacctttcgt
    26701 cgctgcgcgt cattcaggca ggcggcgccc ggctcgaccc gacgcttgct gagcaggtga
    26761 tcgccacctt tgactgtacc ctgcaacagg ttttcggtat ggcggaaggc ctgctctgtt
    26821 ttacccggct ggacgatccg catgccacca ttctccacag ccaggggcgc ccgttgtccc
    26881 ctctggatga aatccgcatc gttgatcaag acgagaacga cgtcgcgccg ggcgaaaccg
    26941 ggcaattgtt aacgcgcggc ccttatacca tttcgggcta ttaccgcgct cctgcccaca
    27001 acgcgcaggc ctttaccgcg caagggtttt accgcacagg cgacaatgtc aggctggatg
    27061 aggtggggaa cctgcacgtt gagggacgca taaaagagca gatcaaccgc gccggagaaa
    27121 aaatagccgc ggctgaagtg gaatcggcac tgctgcgttt agcggaagtg caagattgcg
    27181 cggtggtcgc cgcgccggac acgctgcttg gcgagcggat ttgcgcgttt atcatcgcgc
    27241 agcaggtgcc aactgactat cagcagttgc gtcaacaact gacccgtatg gggctcagcg
    27301 cgtggaaaat tcctgaccaa atcgagtttc tggaccactg gccgctcacc gccgtcggca
    27361 agatagacaa aaaacgcctg acggctctcg ccgtcgaccg ttatcgccat tctgcccaat
    27421 aagcgcaaac cgacccgaaa caggttgaaa taaacccgtt tcgggtagca ctactattag
    27481 aaatagttat cattttcaat tcaccattgt cggtattttt ggcgtttcgc cgtcttacag
    27541 ggactcacaa caatgaaaat gacacggctt tatcctctgg ccttgggggg attattgctc
    27601 cccgccattg ctaatgccca gacttcacag caagacgaaa gcacgctggt ggttaccgcc
    27661 agtaaacaat cttcccgctc ggcatcagcc aacaacgtct cgtctactgt tgtcagcgcg
    27721 ccggaattaa gcgacgccgg cgtcaccgcc agcgacaaac tccccagagt cttgcccggg
    27781 ctcaatattg aaaatagcgg caacatgctt ttttcgacga tctcgctacg cggcgtctct
    27841 tcagcgcagg acttctataa ccccgccgtc accctgtatg tcgatggcgt ccctcagctt
    27901 tccaccaaca ccatccaggc gcttaccgat gtgcaaagcg tggagttgct gcgaggccca
    27961 cagggaacgt tatatggcaa aagcgctcag ggcgggatca tcaacatcgt cacccagcag
    28021 ccggacagca cgccgcgcgg ctatattgaa ggcggcgtca gtagccgcga cagttatcga
    28081 agtaagttca acctgagcgg ccccattcag gatggcctgc tgtacggcag cgtcaccctg
    28141 ttacgccagg ttgatgacgg cgacatgatt aaccccgcga cgggaagcga tgacttaggc
    28201 ggcacccgcg ccagcatagg gaatgtgaaa ctgcgtctgg cgccggacga tcagccctgg
    28261 gaaatgggct ttgccgcctc acgcgaatgt acccgcgcca cccaggacgc ctatgtggga
    28321 tggaatgata ttaagggccg taagctgtcg atcagcgatg gttcaccaga cccgtacatg
    28381 cggcgctgca ctgacagcca gaccctgagt gggaaataca ccaccgatga ctgggttttc
    28441 aacctgatca gcgcctggca gcagcagcat tattcgcgca ccttcccttc aggttcgtta
    28501 atcgtcaata tgcctcagcg ctggaatcag gatgtgcagg agctgcgcgc cgcaaccctg
    28561 ggcgatgcgc gtaccgttga tatggtgttt gggctgtacc ggcagaacac ccgcgagaag
    28621 ttaaattcag cctacgacat gccgacaatg ccttatttaa gcagtaccgg ctataccacc
    28681 gctgaaacgc tggccgcata cagtgacctg acctggcatt taaccgatcg ttttgatatc
    28741 ggcggcggcg tgcgcttctc gcatgataaa tccagtacac aatatcacgg cagcatgctc
    28801 ggcaacccgt ttggcgacca gggtaagagc aatgacgatc aggtgctcgg gcagctatcc
    28861 gcaggctata tgctgaccga tgactggaga gtgtataccc gtgtagccca gggatataaa
    28921 ccttccgggt acaacatcgt gcctactgcg ggtcttgatg ccaaaccgtt cgtcgccgag
    28981 aaatccatca actatgaact tggcacccgc tacgaaaccg ctgacgtcac gctgcaagct
    29041 gcgacgtttt atacccacac caaagacatg cagctttact ctggcccggt cgggatgcag
    29101 acattaagca atgcgggtaa agccgacgcc accggcgttg agcttgaagc gaagtggcgg
    29161 tttgcgccag gctggtcatg ggatatcaat ggcaacgtga tccgttccga attcaccaat
    29221 gacagtgagt tgtatcacga taaccgggtg ccgttcgtac cacgttatgg cgcgggaagc
    29281 agcgtgaacg gcgtgattga tacgcgctat ggcgcactga tgccccgact ggcggttaat
    29341 ctggtcgggc cgcattattt cgatggcgac aaccagttgc ggcaaggcac ctatgccacc
    29401 ctggacagca gcctgggctg gcaggcgact gaacggatga acatttccgt ctatgtcgat
    29461 aacctgttcg accgtcgtta ccgtacctat ggctacatga acggcagcag cgccgtcgcg
    29521 caggtcaata tgggtcgcac cgtcggtatc aatacgcgaa ttgatttctt ctgattattg
    29581 taaaagggat accgaaaagg tatccctttt acaccactag ttaaaaccag taactcagca
    29641 gagtcgcaaa aatattaatc catagtgatt atttaaacaa tgaaattgcg attaggataa
    29701 atagatttaa c
//
LOCUS       DAPHCV010000061        29173 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.061, whole genome shotgun sequence.
ACCESSION   DAPHCV010000061 DAPHCV010000000
VERSION     DAPHCV010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.061"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(204..1679)
                     /gene="ampG"
                     /locus_tag="RH033_002631"
     CDS             complement(204..1679)
                     /gene="ampG"
                     /locus_tag="RH033_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HDV2991798.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLICRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(1723..2301)
                     /locus_tag="RH033_002632"
     CDS             complement(1723..2301)
                     /locus_tag="RH033_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV2991799.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            2606..2923
                     /gene="bolA"
                     /locus_tag="RH033_002633"
     CDS             2606..2923
                     /gene="bolA"
                     /locus_tag="RH033_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HDV2991800.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            3267..4565
                     /gene="tig"
                     /locus_tag="RH033_002634"
     CDS             3267..4565
                     /gene="tig"
                     /locus_tag="RH033_002634"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDV2991801.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            4811..5434
                     /gene="clpP"
                     /locus_tag="RH033_002635"
     CDS             4811..5434
                     /gene="clpP"
                     /locus_tag="RH033_002635"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDV2991802.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            5560..6834
                     /gene="clpX"
                     /locus_tag="RH033_002636"
     CDS             5560..6834
                     /gene="clpX"
                     /locus_tag="RH033_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HDV2991803.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            7022..9376
                     /gene="lon"
                     /locus_tag="RH033_002637"
     CDS             7022..9376
                     /gene="lon"
                     /locus_tag="RH033_002637"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDV2991804.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            9585..9857
                     /gene="hupB"
                     /locus_tag="RH033_002638"
     CDS             9585..9857
                     /gene="hupB"
                     /locus_tag="RH033_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HDV2991805.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            10049..11920
                     /gene="ppiD"
                     /locus_tag="RH033_002639"
     CDS             10049..11920
                     /gene="ppiD"
                     /locus_tag="RH033_002639"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDV2991806.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            12071..12442
                     /locus_tag="RH033_002640"
     CDS             12071..12442
                     /locus_tag="RH033_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDV2991807.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAAPAAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            12548..12946
                     /gene="fadM"
                     /locus_tag="RH033_002641"
     CDS             12548..12946
                     /gene="fadM"
                     /locus_tag="RH033_002641"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HDV2991808.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWHGLENSDSFHWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(12998..13693)
                     /gene="queC"
                     /locus_tag="RH033_002642"
     CDS             complement(12998..13693)
                     /gene="queC"
                     /locus_tag="RH033_002642"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HDV2991809.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(13758..15458)
                     /locus_tag="RH033_002643"
     CDS             complement(13758..15458)
                     /locus_tag="RH033_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HDV2991810.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSADGLRWDFYLRSTLHWHNGDTVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKNLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPQWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            15558..16376
                     /gene="cof"
                     /locus_tag="RH033_002644"
     CDS             15558..16376
                     /gene="cof"
                     /locus_tag="RH033_002644"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HDV2991811.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            16529..16975
                     /gene="decR"
                     /locus_tag="RH033_002645"
     CDS             16529..16975
                     /gene="decR"
                     /locus_tag="RH033_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HDV2991812.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDGLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            17005..18777
                     /locus_tag="RH033_002646"
     CDS             17005..18777
                     /locus_tag="RH033_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDV2991813.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAEDAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIRQFTYPQTDHPALENVNFALKPGQMVGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYNTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAIDA"
     gene            18770..20551
                     /locus_tag="RH033_002647"
     CDS             18770..20551
                     /locus_tag="RH033_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDV2991814.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVIMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIDVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSTLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     EIVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDANTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            20732..21070
                     /gene="glnK"
                     /locus_tag="RH033_002648"
     CDS             20732..21070
                     /gene="glnK"
                     /locus_tag="RH033_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HDV2991815.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            21100..22386
                     /gene="amtB"
                     /locus_tag="RH033_002649"
     CDS             21100..22386
                     /gene="amtB"
                     /locus_tag="RH033_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HDV2991816.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNING
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(22435..23295)
                     /gene="tesB"
                     /locus_tag="RH033_002650"
     CDS             complement(22435..23295)
                     /gene="tesB"
                     /locus_tag="RH033_002650"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HDV2991817.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            23513..24085
                     /locus_tag="RH033_002651"
     CDS             23513..24085
                     /locus_tag="RH033_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HDV2991818.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFNFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATATVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(24116..24427)
                     /locus_tag="RH033_002652"
     CDS             complement(24116..24427)
                     /locus_tag="RH033_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HDV2991819.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            24590..24686
                     /gene="ffs"
                     /locus_tag="RH033_002653"
     ncRNA           24590..24686
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="RH033_002653"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            24806..25159
                     /locus_tag="RH033_002654"
     CDS             24806..25159
                     /locus_tag="RH033_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HDV2991820.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(25201..26751)
                     /gene="pdeB"
                     /locus_tag="RH033_002655"
     CDS             complement(25201..26751)
                     /gene="pdeB"
                     /locus_tag="RH033_002655"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HDV2991821.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELNTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWSSTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(26915..27385)
                     /locus_tag="RH033_002656"
     CDS             complement(26915..27385)
                     /locus_tag="RH033_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HDV2991822.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(27501..28052)
                     /gene="maa"
                     /locus_tag="RH033_002657"
     CDS             complement(27501..28052)
                     /gene="maa"
                     /locus_tag="RH033_002657"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HDV2991823.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(28224..28442)
                     /gene="hha"
                     /locus_tag="RH033_002658"
     CDS             complement(28224..28442)
                     /gene="hha"
                     /locus_tag="RH033_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HDV2991824.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(28468..28842)
                     /gene="tomB"
                     /locus_tag="RH033_002659"
     CDS             complement(28468..28842)
                     /gene="tomB"
                     /locus_tag="RH033_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HDV2991825.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT         7362 a         7000 c         7847 g         6964 t
ORIGIN      
        1 ttatcattaa ggtgacaact gggtggtgag aaaataaaaa ctcattagct gaatcgtgta
       61 agaaaaatag aattcataaa tggcgtcaat aatttccaga cgctaagccg cacaaaagaa
      121 caaatattaa taagcgatgc tgatgtttat ttagccgtaa taattacggc gagtgattac
      181 tacggctaaa taatatttac agattacgtc agatgcgttt ttcgtagcgc cagataatcc
      241 agtaaaccac caagcacgac gccagaaagg gcgactaaaa ccccgacttc cagcagagca
      301 ggcaggaaag agaagtgcgt caaatccagc gcgtccatcg tcaacagcag taaccacacg
      361 gccaacaggc tgacgcccgc cgccagtgtc cacatagcaa aggcataacc tgccggatat
      421 tcggtacggg agataaagtt gtcatttact cgtgtatatt caagcgtctg gcgacaaatc
      481 agcagcaaaa taagccctgg tacggcagcg gcgacggaga acagatagaa tgtcgaccag
      541 ccgtgtgctt caacaaacca acccgccacg gggccgacat aaactcgccc tacagcagaa
      601 agcgctgaga gcagggcaaa ttgagtagcg gaaaatgact tattacacag cgtcattaac
      661 agcgcgacaa aggctgatgt acccatcccg ccacagaggt tttcgaaaaa gacggctgcg
      721 cccatgctgt agagatgctt atcagtaatc gacagcagcc agtaaccggc gttagacgca
      781 ccttgtaaaa taccgaaaat cagcagcgcc cggaacagtg acaggcgctg catcaaaatc
      841 ccaccgtaca atgcgccaac aatggtcgcc agtaagccaa gcgttttgtt aaccacgcct
      901 acttcacccg catcaaaccc gacgccacga atcaaaaacg tggttgtcag gctcatggcg
      961 aatgcgtcgc ccagcttata cagcacgata agaagcaaaa taagccaggc attattgcga
     1021 ccaaagaaat cgcgcagagg tgcaacaacc gcttgttcca gcgttttggg cactggaatg
     1081 gtgtcggtgg gttctggtgc aagtaacgtc gcgataatac aggggatcaa cagcgccgcc
     1141 atcaaccagt acatgccctg ccagcccagc catttatctg ccagccacag ggctaagccg
     1201 ccggaaacca gcatccctaa acggtaaccc agcacgctga ttgccgcgcc cgcaccacgt
     1261 tcttctgccg ggagcacatc ggttttccac gcatcgaaga caatatcctg ggaggcagag
     1321 caaaaagcga tcaccactgc cagtgccgcc atccagcgga gttgggtgcc aggttcgaga
     1381 aaacccatcg ccgcaatggc gactaataac agaatttgcg tggcgagcag ccaaccgcgc
     1441 cgccgcccaa aaaatggggg cgtgtagcgg tccatcagcg gtgaccagag gaatttaaaa
     1501 acgtaagcct ggcctaccag agagaagaaa ccaatggttt tgagatcgat attctcgacc
     1561 gtcatccagg cctgtaaggt gccggacgtc agggcgagcg gtagcccgga agcaaaaccc
     1621 aggatcagca atatggctga acgtggctgt tgaaaaatac gtaaatattg actggacatg
     1681 ggcgtctaca ggcccggctt gcaccgggcc agagggcaga aattaacgcg cgttctgctt
     1741 gatgaattcg tggatgctgg tgtcctgaga catatcagcg atggtatcgg tcagcacact
     1801 gttaaccgca tcggcgatat ttttgttgga ggcctggaac gcaccttcaa cgttgtagct
     1861 ggcacgatag tttttggtca ttttgttgcc attctgcgcg gtagcgatga tggcgatatc
     1921 cgctttagta gcgatgttgt agcgcacgtt gccctgggac acgtcagcat acaattggct
     1981 aacgatgatt tgcagattaa ccgggccatt cggaccaacc atgtaaccac gcgcggtcat
     2041 ctgtttttcc agcacttctt gcagcaggaa acgcagatcg cgggaggcgg tcagggtaac
     2101 gatttgatta tcgcgggtga cttttgccag cgcctgatcg gtacgctgat cggcaccatt
     2161 aatgcttacg gtgacgccca tcaggcttgg atcctgctgt ggcagtgtaa tcgtcgggga
     2221 aacttcaata gttgttggcg gttttgcgca tcctgcaagc ataaacagag caactaacgg
     2281 gaagaggatt tttttgaaca tgttcgggct ctcagagact cttaagcgtg tttggtaaaa
     2341 attcccgcca tcataacatt gccaacggcg aggggaagtg ggtaaggcat gtaaattcat
     2401 catgttgacg aaataatcac ccctggtaaa agaaacgctg atgcgaggcc tgtgtttcaa
     2461 tctttaaatc agtaaacttc atacgcttga cagaaaaacc aggacgaaac ctaaatattt
     2521 gttgttaagc tgcaatggaa acggtaaaag cggctagtat ttaaagggat ggatgacatc
     2581 tcagcgttgt cggaggagat atttcatgat gatacgtgag cggatagaag aaaaattaag
     2641 ggcggcgttc caacccgtat tcctcgaagt agtggatgaa agctatcgtc acaatgtccc
     2701 agccggctct gaaagccatt ttaaagttgt gctggtcagc gatcgtttta cgggtgaacg
     2761 ttttctgaat cgtcatcgaa tgatttacag tactttagcg gaggaactct ctactaccgt
     2821 tcatgcgctg gctctgcata cttacactat taaggagtgg gaagggttgc aggacaccgt
     2881 ctttgcctct cctccctgtc gtggagcagg aagcatcgcg taaaaacgca tttgcaactg
     2941 tcggcgcttt tccagtatgt tgctaaagat tttatgaaaa acggcctgcg ggccgttttg
     3001 ttttgtctgg attttgcgct ttttgcccag cattcagacg aaaattgccc gggaattgtg
     3061 aaaaaatacg cgacagcgcg caataaccgt tctcgactca taaaagtgat gccgctataa
     3121 tgccgcgtcc tatttgaatg ctttcgggat gattctggta acagggaatg tgattgatta
     3181 taagaacatc ccggttccgc gaagccaaca acctgtgctt gcggggtaag agttgaccga
     3241 gcactgtgat tttttgaggt aacaagatgc aagtttcagt tgaaaccact caaggccttg
     3301 gccgccgtgt aacgattact atcgctgctg acagcatcga gaccgctgtt aaaagcgagc
     3361 tggtcaacgt tgcgaaaaaa gtacgtattg acggcttccg caaaggcaaa gtgccaatga
     3421 atatcgttgc tcagcgttat ggcgcgtctg tacgccagga cgttctgggt gacctgatga
     3481 gccgtaactt cattgacgcc atcattaaag aaaaaatcaa tccggctggc gcaccgactt
     3541 atgttccggg cgaatacaag ctgggtgaag acttcaccta ctctgtagag tttgaagttt
     3601 atccggaagt tgaactgcag ggtctggaag cgatcgaagt tgaaaaaccg atcgttgaag
     3661 tgaccgacgc tgacgttgac ggcatgctgg atactctgcg taaacagcag gcgacctgga
     3721 aagaaaaaga cggcgctgtt gaagcagaag accgcgtaac catcgacttc accggttctg
     3781 tagacggcga agagttcgaa ggcggtaaag cgtctgattt cgtactggcg atgggccagg
     3841 gtcgtatgat cccgggcttt gaagacggta tcaaaggcca caaagctggc gaagagttca
     3901 ccatcgacgt gaccttcccg gaagaatacc acgcagaaaa cctgaaaggt aaagcagcga
     3961 aattcgctat caacctgaag aaagttgaag agcgtgaact gccggaactg actgcagaat
     4021 tcatcaaacg tttcggcgtt gaagatggtt ccgtagaagg tctgcgcgct gaagtgcgta
     4081 aaaacatgga gcgcgagctg aagagcgcca tccgtaaccg cgttaagtct caggcgatcg
     4141 aaggtctggt aaaagctaac gacatcgacg taccggctgc gctgatcgac agtgaaatcg
     4201 acgttctgcg tcgccaggct gcacagcgtt tcggtggcaa cgaaaaacaa gctctggaac
     4261 tgccgcgcga actgttcgaa gaacaggcta aacgtcgcgt agttgttggc ctgctgctgg
     4321 gcgaagttat ccgcaccaac gagctgaaag ctgacgaaga gcgcgtgaaa ggcctgatcg
     4381 aagagatggc ttctgcgtac gaagatccga aagaagttat cgagttctac agcaaaaaca
     4441 aagaactgat ggacaacatg cgcaatgttg ctctggaaga acaggctgtt gaggctgtac
     4501 tggcgaaagc gaaagtgact gaaaaagaaa ccactttcaa cgagctgatg aaccagcagg
     4561 cgtaatttac gcagcataac gcgctaaatt cgcacaaagg cccgtcaccg ccaggtggtg
     4621 ggcttttttt tgtcatgaat tttgcatgga accgtgcgaa aagcctcttt cggtgttagc
     4681 gtaacaacaa aagattgtta tgcttgaaat atggtgatgc cgtacccata acacagggac
     4741 tagctgataa tccgtccata aggttacaat cggtacagca ggttttttca attttatcca
     4801 ggagacggaa atgtcataca gcggcgaacg agataacttt gcaccccata tggcgctggt
     4861 gccgatggtc attgaacaga cctcacgtgg tgagcgctct tttgatatct attctcgtct
     4921 acttaaggaa cgcgtcattt ttctgactgg ccaggttgaa gaccacatgg ctaacctgat
     4981 tgtggcgcag atgctgttcc tggaagcaga aaacccagaa aaagatatct atctgtacat
     5041 taactcccca ggcggggtga tcactgccgg gatgtctatc tatgacacca tgcagtttat
     5101 caagcctgat gtcagcacca tctgtatggg ccaggcggct tcgatgggcg ctttcttgct
     5161 gactgcaggg gcaaaaggta aacgtttctg cctgccgaat tcgcgcgtga tgattcacca
     5221 gccgttgggc ggctaccagg gccaggcgac cgatatcgaa attcatgccc gtgaaattct
     5281 gaaagttaaa gggcgcatga atgaacttat ggcgcttcat acgggtcaat cattagaaca
     5341 gattgaacgt gataccgagc gcgatcgctt cctttccgcc cctgaagcgg tggaatacgg
     5401 tctggtcgat tcgattctga cccatcgtaa ttgatgccag aggcgcaact gtgccgctat
     5461 acttatccag ggcggcacaa cgctgtaagc ggcttgcgcc tgagaatggc atttgcgtcg
     5521 tcgtgtgcgg cacaaagaac aaagaagagg ttttgaccca tgacagataa acgcaaagat
     5581 ggctcaggca aattgctgta ttgctctttt tgcggcaaaa gccagcatga agtgcgcaag
     5641 ctgattgccg gtccatccgt gtatatctgc gacgaatgtg ttgatttatg taacgacatc
     5701 attcgcgaag agattaaaga agttgcaccg catcgtgaac gcagtgcgct accgacgccg
     5761 catgaaattc gcaaccacct ggacgattac gttatcggcc aggaacaggc gaaaaaagtg
     5821 ctggcggtcg cggtatacaa ccactacaaa cgtctgcgca acggcgatac cagcaatggt
     5881 gtcgagttgg gcaaaagtaa cattctgctg atcggtccga ccggttccgg taaaacgctg
     5941 ctggctgaaa cgctggcgcg cctgctggac gtcccgttca ccatggccga cgcaaccacg
     6001 ctgaccgaag ccggttatgt gggcgaagac gttgaaaaca tcattcagaa gctgttgcag
     6061 aagtgcgatt acgacgtaca gaaagcgcag cgcgggattg tctacatcga tgaaatcgac
     6121 aagatttctc gtaagtcaga caacccgtcc attacccgag acgtttccgg tgaaggcgta
     6181 cagcaggcac tgttgaaact gatcgaaggt acggtagctg ctgttccacc gcaaggtggg
     6241 cgtaaacatc cgcagcagga attcttgcag gttgatacct ctaagatcct gtttatttgt
     6301 ggcggtgcgt ttgccggtct ggataaagtg atttcccatc gtgtagaaac cggctccggc
     6361 attggttttg gcgcgacggt aaaagcgaag tccgacaaag caagcgaagg cgagctgctg
     6421 gcgcaggttg aaccggaaga tctgatcaag tttggtctta tccctgagtt tattggtcgt
     6481 ctgccggttg tcgcaacgtt gaatgaactg agcgaagaag ctctgattca gatcctcaaa
     6541 gagccgaaaa acgccctgac caagcagtat caggcgctgt ttaatctgga aggcgtggat
     6601 ctggaattcc gtgacgaggc gctggatgct atcgctaaga aagcgatggc gcgtaaaacc
     6661 ggtgcccgtg gcctgcgttc catcgtagaa gccgcactgc tcgataccat gtacgatctg
     6721 ccgtccatgg aagacgtcga aaaagtggtt atcgacgaat cggtaattga tggtcaaagc
     6781 aagccgttgc tgatttatgg caagccggaa gcgcaacagg catctggtga ataattaacc
     6841 attcccatac aattagttaa ccaaaaaggg gggattttat ctccccttta atttttcctc
     6901 tattctcggc gttgaatgtg ggggaaacat ccccatatac tgacgtacat gttaatagat
     6961 ggcgtgaagc acagtcgtgt catctgatta cctggcggaa attaaactaa gagagagctc
     7021 tatgaatcct gagcgttctg aacgcattga aatccccgta ttgccgctgc gcgatgtggt
     7081 ggtttatccg cacatggtca tccccttatt tgtcgggcgg gaaaaatcta tccgttgtct
     7141 ggaagcggcg atggaccatg ataaaaaaat tatgctggtc gcgcagaaag aagcttcaac
     7201 ggatgagccg ggtgtaaacg atcttttcac cgtcgggacc gtggcctcta tattgcagat
     7261 gctgaaactg cctgacggca ccgtcaaagt gctggtcgag gggttacagc gcgcgcgtat
     7321 ttctgcgctc tctgacaatg gcgaacactt ttctgcgaag gcggagtatc tggagtcgcc
     7381 gaccattgat gagcgggaac aggaagtgct ggtgcgtact gcaatcagcc agttcgaagg
     7441 ctacatcaag ctgaacaaaa aaatcccacc agaagtgctg acgtcgctga atagcatcga
     7501 cgacccggcg cgtctggcgg ataccattgc tgcacatatg ccactgaaac tggctgacaa
     7561 acagtccgtt ctggagatgt ccgacgttaa cgaacgtctg gaatatctga tggcaatgat
     7621 ggaatcggaa atcgatctgc tgcaggttga gaaacgcatt cgcaaccgcg ttaaaaagca
     7681 gatggagaaa tcccagcgtg agtactatct gaacgagcaa atgaaagcta ttcagaaaga
     7741 actcggtgaa atggacgacg cgccggacga aaacgaagcc ctgaagcgca aaatcgacgc
     7801 ggcgaaaatg ccgaaagagg caaaagagaa agcggaagca gagttgcaga agctgaaaat
     7861 gatgtctccg atgtcggcag aagcgaccgt agtgcgtggt tatatcgact ggatggtaca
     7921 ggtaccgtgg aatgcgcgta gcaaggtcaa aaaagacctg cgtcaggcgc aggaaatcct
     7981 tgataccgac cattatggtc tggagcgcgt gaaagatcgc atccttgagt atcttgcggt
     8041 tcaaagccgt gtcaacaaaa tcaagggacc gatcctctgc ctggtagggc cgccgggggt
     8101 aggtaaaacg tccctggggc agtccatcgc caaagccacc gggcgtaaat atgtccgtat
     8161 ggcgctgggc ggcgtgcgtg acgaagcgga aatccgtggt caccgccgta cttacatcgg
     8221 ttctatgccg ggtaaactga tccagaaaat ggcgaaagtg ggcgtgaaaa acccgctgtt
     8281 cctgctcgat gagatcgaca aaatgtcttc tgacatgcgt ggcgatccgg cctccgcact
     8341 gcttgaagtg ctggatccag agcagaacgt agcgttcagc gaccactacc tggaagtgga
     8401 ttacgacctt agcgacgtga tgtttgtcgc gacgtcgaac tccatgaaca ttccggcacc
     8461 gctgctggat cgtatggaag tgattcgcct ctccggttat accgaagatg aaaaactgaa
     8521 catcgccaaa cgtcacctgc tgccgaagca gattgaacgt aatgcactga aaaaaggtga
     8581 gctgaccgtc gacgatagcg ccattatcgg cattattcgt tactacaccc gtgaggcggg
     8641 cgtgcgtggt ctggagcgtg aaatctccaa actgtgtcgc aaagcggtta agcagttact
     8701 gctcgataag tcattaaaac atatcgaaat taacggcgat aacctgcatg actatctcgg
     8761 tgttcagcgc ttcgactatg gtcgcgcgga taacgaaaac cgtgtcggtc aggtaaccgg
     8821 tctggcgtgg acggaagtgg gcggtgactt gctgaccatt gaaaccgcgt gcgttccggg
     8881 gaaaggcaaa ctgacctata ccggttcgct cggtgaagtg atgcaggagt ccattcaggc
     8941 ggcgttaacg gtggttcgtg cgcgtgcgga aaaactgggg atcaaccctg atttttacga
     9001 aaaacgtgac atccacgtcc acgtaccgga aggtgcgacg ccgaaagatg gtccgagtgc
     9061 cggtattgct atgtgtaccg cgctggtttc ttgcctgacc ggtaacccgg ttcgtgccga
     9121 tgtggcaatg accggtgaga tcactctgcg tggtcaggta ctgccgatcg gtggtttgaa
     9181 agaaaaacta ctggcagcgc atcgcggcgg gattaaaaca gtgctaattc cgttcgaaaa
     9241 taaacgcgat ctggaagaga ttcctgacaa cgtaattgcc gatctggaca ttcatcctgt
     9301 gaagcgcatt gaggaagttc tcactctggc gctgcaaaat gaaccgtctg gcatgcaggt
     9361 tgtgactgca aaatagtgac ctcgcgcaaa atgcactaat aaaaacaggg ctggcaggct
     9421 aattcggact tgccagcctt tttttgtctc gctaagttag atggcggatt gggcttgccc
     9481 ttattaaggg gtgttgtaag gggatggctg gcctgatata actgctgcgc gttcgtacct
     9541 tgaaggattc aagtgcgata taaattataa agaggaagag aagagtgaat aaatctcaat
     9601 tgatcgacaa gattgctgca ggggctgata tctctaaagc tgcggctggc cgtgcgttag
     9661 atgctattat tgcttccgta actgaatctc tgaaagaagg ggatgatgta gcactggtag
     9721 gttttggtac ttttgccgtt aaagagcgtg ctgcccgtac tggccgcaac ccgcagaccg
     9781 gtaaagagat caccatcgct gctgctaaag taccgagctt ccgtgcaggt aaagcactga
     9841 aagacgcggt aaactaagcg ttgtccccag tggggatgtg acgaagttca agggcgcatc
     9901 tactgatgtg ccttttttat ttgtattcgg tgactttctg cgtcttgtgg gctgacaatt
     9961 gcccccgttt cttgtcacaa tatgcctttg cgcgcatcga tacgttgcgt gaggtacaca
    10021 gtcatctaca gcggagtgtt gttacaccat gatggacagc ttacgcacgg ctgcaaacag
    10081 tctcgtgctc aagattattt tcggtatcat tatcgtgtcg ttcatattga ccggcgtgag
    10141 tggttacctg attggcggag gcaataacta cgccgcaaaa gtgaatgacc aggaaatcag
    10201 tcgtgggcaa ttcgagaacg ccttcaacag cgagcgtaat cgcatgcagc aacagctggg
    10261 cgatcaatac tccgagctgg cagcgaacga aggctatatg aaaaccctgc gtcaacaggt
    10321 gctgaatcgt ctgatcgacg aggcgctgct ggatcagtac gctcgtgagc tgaaactggg
    10381 tatcagcgat gagcaggtta aacaggcgat tttcgcgacc ccagccttcc aggttgacgg
    10441 caaatttgat aacagccgct ataacggtat cctcaaccag atggggatga ccgccgatca
    10501 gtacgcccag gcgctgcgta accagctcac aacccaacag ctgattaacg gcgttgccgg
    10561 taccgatttt atgctgaaag gtgaaaccga cgagctggcg gcactggtag ctcaacaacg
    10621 cgtggtgcgt gaagcgacta tcgatgttaa cgcgctggcg gcgaagcagc ctgtgaccga
    10681 acaggaaatt gccagctact acgaacaaaa caaaaacaat ttcatgacgc cggaacaatt
    10741 ccgcgtgagt tacatcaagc tggatgccgc gacgatgcag caaccggtta gcgatgcgga
    10801 tatccagagc tactacgatc agcatcagga tcaattcacc cagccgcagc gtacccgtta
    10861 cagcatcatc cagaccaaaa ctgaagatga agcgaaagcg gtacttgatg agctgaataa
    10921 gggcggtgat tttgctgcat tagccaaaga aaaatctgcc gatattatct ctgcccgcaa
    10981 cggcggtgat atgggttggt tagaagatgc cactatcccg gacgaactga aaaatgctgg
    11041 tctgaaagaa aaaggccaac tgtctggtgt tatcaaatct tcggtcggtt tcctgattgt
    11101 gcgtctggac gacattcagc cagcgaaagt gaaatcgtta gacgaagtac gtgacgacat
    11161 tgcggcgaaa gtgaaacacg aaaaagccct cgatgcgtac tacgcgctgc agcagaaagt
    11221 gagcgatgcg gcaagcaacg acaccgagtc tctggccggt gcagagcaag ctgccggcgt
    11281 taaagccact cagacgggtt ggttcagcaa agataacctg ccggaagagt tgaacttcaa
    11341 gccagttgcc gacgctatct tcaacggcgg tctggtaggt gaaaacggcg cgccgggcat
    11401 caactctgac atcatcaccg tagacggcga ccgcgcattc gtgctgcgca tcagcgagca
    11461 caaaccggaa gcggtgaaac cgttggcaga tgttcaggaa caagttaagg cactggttca
    11521 gcacaacaaa gctgaacaac aggcgaaagt ggatgctgag aaactgctgg ttgatttgaa
    11581 agcaggcaaa ggtgcggaag ctatgcaggc tgccggtctg aaatttggcg agccgaaaac
    11641 cttaagtcgt tccggtcgtg acccgattag ccaggctgcg tttgcactgc cgctgccagc
    11701 gaaagacaaa ccgagctacg gtatggcgac cgatatgcaa ggtaatgtag ttctgctggc
    11761 gctggatgaa gtgaagcaag gttcaatgcc ggaagatcag aaaaaagcga tggtgcaggg
    11821 tatcacccag aacaacgcac aaatcgtctt tgaagctctg atgagtaacc tgcgtaaaga
    11881 ggcgaaaatc aaaattggcg atgcgctgga acagcaataa tcctgaagcc gcctcgcaaa
    11941 aaaatgcttt gcagctgtaa caagaaaagg tcgctttcgc ggccttttcc atttctgaac
    12001 attgccattt gtttactgtt ttcactgccg ttaaggtgat tccactgtta acaaacaagg
    12061 agaaaacagt atgaaacacg gaattaaagc actgctcatt accctgtccc tggcctgtgc
    12121 cggaatgtct catagtgctc tggcggcagc tcctgcggca aaaccgacaa cggtagaaac
    12181 caaagcggaa gctcctgcag cacaaagtaa agcagcagtt ccggcgaaag ccagtgacga
    12241 agaaggcacc cgggtcagca ttaataatgc cagcgcggaa gagttagccc gcgcgatgaa
    12301 tggcgttggc ctgaagaaag cgcaggcgat tgtcagttat cgcgaagagt acggtccgtt
    12361 taaaacggtg gaggatctca agcaggtgcc ggggatgggc aattcgctgg tggaacgtaa
    12421 tctggcggta ttaaccctgt aattaatttg catagtggca attttttgcc agactgaaga
    12481 ggtcatacca gttatgacct ctgtacttat aacagttctt ataacaacaa cgtaaggtta
    12541 ttgcgctatg caaacacaaa tcaaagttcg tggatatcat ctcgacgttt accagcacgt
    12601 aaacaacgcc cgctaccttg aatttctcga agaagcccgc tggcatgggt tggaaaatag
    12661 cgacagtttt cactggatga cggcccataa catcgctttc gtcgtggtca atatcaatat
    12721 taactatcgc cgcccggcgg tattaagcga cctgttaacc attaccagtc agttgcagca
    12781 attaaacggt aaaagtggca tcttaagcca ggtcattaca ctggagccgg aaggacaggt
    12841 ggtagcggat gcgcttatta cgtttgtttg tattgatctt aaaacgcaga aagcattagc
    12901 tctggaaggg gaattgcgcg aaaagctgga gcagatggtt aagtaaacgt tttgtggtgc
    12961 cggatgctca agccgcatcc ggcgacaccc ggaataatta cttcaacccg gttttctgct
    13021 tcatcgctgc catcaccgtc ggtttatcgg ccagataatg attcaaaccg ttggcacgta
    13081 aattacacgc cgcacaatga ccgcaaccgt cgcctttaat gccgttataa caggtcagcg
    13141 tttcgttacg gactaaatcc agcttgccgt aataatctgc cagcgcccag gtttccgctt
    13201 tatcaatcca catcagcggc gtttcaaaac gaatatcttt cgccatgccc aaactgacgg
    13261 catggtttag tgctttcaca aactcatcgc ggcaatccgg gtagccggag aaatccgttt
    13321 cgcagacgcc ggtaattacg gcttctgctt ttacctgata cgcatatatt gccgccagtg
    13381 tcaggaacaa aatattacgc cctgggacaa acgtattcgg gataccatcg gcttcaggtt
    13441 cataatcagg caccggaatg ctgtcacgcg tcaggctgct gaccgccagc tcgttgagca
    13501 gcgttacatc aagcacctta tgcgcgcgtg cccccagttt cagcgccagt tcgcgtgcca
    13561 cgtcgatttc tgcgcgatgg cgttgaccgt aatcgaacgt cacgcaatgg acttcatcat
    13621 attgttgtaa tgcctgcacc agacaggtgg tggaatcctg gcctccactg aacacaacga
    13681 cagcacgttt catagataat cctgcttgaa caataaaagc gttatggtaa cgcctgcgat
    13741 taacccggac cagctattca ttgcgacggc gcgggaagcc aggcttcggt aaattcaaac
    13801 cagccgcgcg gtgtcagtcg cacaccgttc acgccgggag gggcgctaat gcgatagtga
    13861 tagttgaaca gcggcgtcag cgtcgcatct gccattaact ggctaaaaac cgctttcagg
    13921 gcattaaatc ggttttcctc atcaggcatt acttgcaccg cgtccagtgt cgattgcaaa
    13981 tgtgcatatg ctggagcgtc gaaaacatgt ggccacagtg gatcgcaacg cagccattgc
    14041 tccagggtat attccggtgc ttcgccaatt aatctgtcgc ccatcatgag gtctgcgtgt
    14101 gccagtaggg tcgtatcgtc ccagtttttt gcgttatgaa aaataattgt aagttcacaa
    14161 ccttccgctg ccagtgtcgc ctgtaggcgt tctgccatgg tatgaagttc tatcggtagg
    14221 tgataaacca gcgtcaaggt tttcggtagt ttaacttcat ccggtacctg ccattgtgga
    14281 atagtccagc ctggcagtaa tgcatgactg gcggtgatca ggttttctcc gacttctaac
    14341 gtttgtaata aaccggattg atgaataatg gagatcactt ttcgcgcctg ccagagggag
    14401 agtcggggac ttttgcgcaa cgttaaatag caaaaaccta aactgatgcc actactgacc
    14461 tggctgaccc gttgcagctc ctccggtttg ccgatggtga tttgcacggg atgccgacaa
    14521 ctggttccca aatttttttc gaaaagtggc ggagttatcc agtactcaac cgctttaagc
    14581 agcggatgac gtaaatggta ataatcatgg ctctccaggc gcaccagttc cgctgtgaat
    14641 tgtgttaagc gaaaaggacc tgtgccgatc agtgggaatt gcggatgcgc cagatggctg
    14701 caatagctcg ccagccggtg cgcaagccag taatcggggc gatgtaaaaa gaaggtcaga
    14761 cactgcggat gggtgacttc aatacgcttc acgctaataa ataattgatc cagtgctggc
    14821 agttgtaaca gcatcaataa tcgctggtgt aagtgtgagg cttttactgt atcgccgtta
    14881 tgccagtgta gggttgaacg aagataaaag tcccagcgta acccgtcagc agaggtttcc
    14941 cagtgatgcg ctaaatcgcc aatcgggcgc tgggtattat tatcgaagcg ggtcaggccg
    15001 gaaaatatct gtccggcgag atgctgctcg gcacggccgg gcaaaaagcc cggttgtagc
    15061 ggttcgagcg ggcgatagta gggaatacgt aacgtgggcg tatcgttttg ccattgtccg
    15121 cccataaacg gctgtaacag agtgcgcagc tcacctgggg ccagttgcgc cagctccagc
    15181 acatcttgct gctttccggt ttccagtgcc tgttccatca tcgcattgcg tagcgattct
    15241 ggcgtgacca gaaagcgtag ttgcccgcgc tttccgcgtc ctgactgcgc ctgccactcc
    15301 agccatcccg cctcctgtgc ctgacgcaac agcgtacgaa catggcgttc gctgcaaaaa
    15361 cagcgttcgg ccagttcgct gacggtgacg gtttgcggct ttccggcgga aggttgccac
    15421 agacgttgat actggttaag acggttgagc agtcgcatat aaacccggaa caatattatt
    15481 taactattca ctattacttc cgtatatatc aggtgatact caatcaccat taaccgtgtc
    15541 acagagtgga gaaagaaatg gctcgtctgg cagcatttga tatggatggc actttattga
    15601 tgcctgacca tcatttaggt gagaaaaccc tctctacttt ggcgcggctg cgtgaacgcg
    15661 acattaccct cacttttgcc acggggcgtc atgcgctgga gatgcagcat attctcgggg
    15721 cgctatcgct ggatgcatat ttgattactg gcaacggaac gcgcgtacat tctctggaag
    15781 gtgaactttt acatcgtgat gatttacctg cggatgtcgc ggagctggtg ctgtatcagc
    15841 aatgggatac ccgagcaagc atgcatatct tcaatgacga tggttggttt accgggaaag
    15901 agatccctgc gttgttgcag gcatttgtct atagcggttt tcgttatcag ataatcgatg
    15961 tcaaaaaaat gccactcggc agcgtcacca agatctgctt ctgtggcgat cacgacgatc
    16021 ttacacgctt gcagatccag ctatacgaag cattaggtga gcgtgcacat ttatgttttt
    16081 ccgccacgga ttgcctcgaa gtgctgccgg tgggctgcaa taaaggcgct gcattgacgg
    16141 tgctgaccca acatttaggt ttatcgttac gcgattgcat ggcctttggt gatgcgatga
    16201 acgatcgcga aatgttaggc agtgtcggta gcggatttat tatgggcaat gcgatgccgc
    16261 aactgcgcgc ggagctcccg catttaccgg tgattggaca ttgccgaaat caggctgtct
    16321 ctcactattt gacgcactgg ctggactatc cacatctacc ttattccccc gaataacgag
    16381 atcccttcca gcaccgggca attgcccggt tttttttgcg ttgaatttgt cattttgtgc
    16441 cgtggtgttt aaaccgcaca gaataaattg tcgcgatttc acctttaaaa tagaattaaa
    16501 agagaaaaaa attctctgtg gaagggctat gttagataaa attgaccgta agctgctggc
    16561 cttactacag caggattgca ccctctcttt gcaggcactg gctgaagccg ttaatctgac
    16621 aaccaccccg tgctggaagc gtctgaaacg gctggaggac gacggtattc ttatcggcaa
    16681 agtcgccctg ctggatccgg aaaaaatagg cctcggcctg accgcctttg tgctgataaa
    16741 aacgcaacat cacagcagcg aatggtattg ccgctttgtc acggtggtta ccgaaatgcc
    16801 agaagtgctg gggttctggc gcatggccgg tgaatacgat tatctgatgc gcgtccaggt
    16861 tgccgacatg aaacgctacg acggtctggt aaacagcgtg ccggggctgt cggacgtcac
    16921 ttccagcttc gcgatggaac agattaaata caccacttct ttacccatcg aataaatatc
    16981 cagaatcagg tcaggacaca acgcgtgcga ttatttgctc aattaagctg gtatttccgt
    17041 cgggaatggc gtcgctatct cggggctgtc gccttgcttg tcattatcgc gatgctgcaa
    17101 ctggttccgc caaaagtggt tggtattgtt gttgatggcg tgacagaaca acactttact
    17161 accgggcaga tcctgatgtg gatcgccacc atggtgctga ttgccgttgt ggtttatctc
    17221 ctgcgttacg tctggcgcgt attgctgttt ggtgcgtctt atcaactggc tgttgaactg
    17281 cgtgaagatt attaccgtca gctaagccgg cagcatcctg agttttacct gcgtcatcgc
    17341 acgggtgacc tcatggctcg tgcgaccaat gacgtcgatc gcgtcgtgtt tgccgccgga
    17401 gaaggggtgc tgacgctggt ggattcactg gtaatgggct gcgctgtgtt gattatgatg
    17461 tctacgcaaa ttagctggca gttgacctta tttgccctgt tgccgatgcc agtgatggcg
    17521 atcatgatta agcgcaacgg cgatgccttg catgaacgct ttaagctggc gcaggcggcg
    17581 ttttccagtc ttaatgaccg cacccaggaa agcctcacca gtatccgcat gatcaaagct
    17641 tttggtctgg aagatcgcca gtcggcgtta tttgccgagg atgccgaaga taccggcaaa
    17701 aaaaacatgc gggtggcgcg tattgatgct cgtttcgacc cgaccatcta tatcgcgatt
    17761 ggtatggcga acttgctggc gattggcggc ggtagttgga tggtggtgca gggcagttta
    17821 acgctgggcc agctcaccag ttttatgatg tatttaggtc tgatgatttg gccaatgctg
    17881 gcgctggcat ggatgtttaa catagtggaa cgtggtagtg ctgcgtacag ccgcattcgc
    17941 gcgatgctgg cggaagcgcc ggtggtgaac gatggtagcg aacccgtgcc ggaaggtcgt
    18001 ggcgaactgg atgtaaatat tcgccagttc acgtatccgc agactgacca tcctgcgctg
    18061 gaaaacgtca atttcgccct gaaacccggt cagatggtag gtatttgcgg gccgactggt
    18121 tccggcaaaa gtaccctgtt gtcgctcatt cagcgtcatt tcgacgtcag cgagggggat
    18181 atccgctttc atgatattcc tctgacgaag ttacaactcg atagctggcg tagccgcctg
    18241 gcggtagtta gccagacacc gttccttttt tccgacaccg tagcgaacaa catcgcgttg
    18301 ggttgcccga atgccaccca gcaagagatt gagcatgtcg cgcggttagc cagcgtgcat
    18361 gacgatattt tgcgtttacc gcaaggttac aatacagaag tgggcgagcg cggcgtgatg
    18421 ctttccggtg ggcaaaaaca gcgtatctcc attgctcgtg cgttattagt caacgcggaa
    18481 atcctcatcc ttgatgatgc gctttcggca gtagacggac gcacagagca ccagatcctg
    18541 cataacctgc gtcagtgggg gcagggaaga acggtaatca tcagcgccca tcgcctttct
    18601 gcactgacgg aagccagtga aattattgtg atgcagcacg gacatatcgc tcagcgtggc
    18661 aatcatgatg agctggcgca acaaagcggc tggtatcgcg atatgtatcg ctatcaacaa
    18721 ctggaggcgg cgctcgacga cgctccggaa attcgcgagg aggccatcga tgcgtagttt
    18781 tagccaactg tggccgactc tcaagcgtct gttagcgtac ggttcgccgt ggcgtaaacc
    18841 gctggggatt gcggtcataa tgatgtgggt tgcggcagcg gcagaagtca gtgggccgct
    18901 gcttatcagc tattttatcg acaatatggt agcgaaaaat aacctgccgt tgaaagtggt
    18961 tgcagggctg gctgcggcgt atgttgggct gcaactgttt gccgccgggc tacattacgc
    19021 gcagtcgctg ctgtttaatc gggcggcagt aggcgtagtg caacagttgc gtaccgacgt
    19081 gatggatgct gcgttacgcc agccattaag cgagtttgat acccaacccg tcgggcaggt
    19141 gatttcccgc gtcactaatg acactgaagt gatccgcgac ctctacgtta ccgtagtggc
    19201 aactgtcctg cgtagcgccg cactggtggg cgcgatgctg gtggcgatgt tcagcctcga
    19261 ctggcgaatg gcgctggtgg cgataatgat tttcccggtg gtgctggtgg taatggtgat
    19321 atatcagcgt tacagcacgc cgattgtccg tcgtgtgcgc gcttatctgg cagatatcaa
    19381 cgacggcttt aacgaaatta tcaatggcat gagcgttatc cagcagttcc gtcagcaggc
    19441 gcgatttggc gaacgtatgg gtgaggccag tcgttcacac tatatggcga ggatgcaaac
    19501 cctgcgcctc gacggttttc tgctgcgtcc gctgctgagt ctgttttcat cgctcattct
    19561 ttgtggcttg ttgatgctgt ttggcttctc cgccagcggc accattgaag tgggcgtgtt
    19621 gtatgcgttc atcagttatt tggggcgact taacgaaccc ttaatcgaac tgaccacgca
    19681 gcaggcgatg ctgcaacagg ctgttgttgc tggtgagcgc gtttttgagc tgatggacgg
    19741 accgcgccag caatatggca atgatgatcg cccgttgcag agcggcacca tcgacgtcga
    19801 taacgtgtca tttgcttatc gcgatgacaa tctggtgcta aagaacatta atctctctgt
    19861 gccttcgcgc aactttgtgg cgctggtcgg gcataccggc agtggcaaaa gcaccctcgc
    19921 cagtttattg atgggctatt acccgctaac ggaaggtgag attcgccttg atggtcgtcc
    19981 attaagtacg ctaagtcaca gcgcgctgcg ccagggcgtg gcaatggtgc agcaagatcc
    20041 ggtggtgctg gcggatacct tcctcgccaa cgtgacgctg ggacgtgata tctctgaaga
    20101 acgcgtctgg caggcgctgg aaatcgtgca gctggcggag ctggcgcgta gcatgagtga
    20161 tggtatttac acgccgcttg gcgagcaggg gaataatctc tcagtcgggc aaaagcaact
    20221 gctggcactg gcgcgcgtgc tggtggagac gccgcaaatc ctgatccttg atgaggcaac
    20281 cgccagcatt gactccggga ctgaacaggc gattcaacat gctctggcgg cggtgcgtga
    20341 acatactacg cttgtggtga ttgctcaccg cttatcgact attgttgatg ccaacaccat
    20401 tctggtgctt catcgtgggc aagccgtgga gcagggcact caccagcaac tgctggcggc
    20461 ccagggacga tactggcaga tgtatcaact gcaacttgcg ggcgaagagc tggcagccag
    20521 cgtgcgtgaa gaggaatcat tgagcgcctg aatagcgcaa tatttcatcg ttggtgcaaa
    20581 aatgtaacgc actgtgcact gtcatagtgc gttttcattt tcaatcttct taacttcctg
    20641 ctctctttct cgtttttcat ttttggcaca ccgcttgcaa taccttcttc gtgtagcaga
    20701 accattaccg aattctgacc ggaggggatc tatgaagctg gtgaccgtga ttatcaaacc
    20761 attcaaactt gaagacgttc gtgaagcgtt atcttccatt ggtattcagg gcctgaccgt
    20821 caccgaagtg aaaggtttcg ggcgtcagaa aggacatgcc gagctgtacc ggggggcgga
    20881 atacagcgtc aatttcctgc caaaagtaaa aattgatgtg gcgattgctg atgaccaact
    20941 cgatgaagtg atcgatatcg tcagtaaggc ggcttacacc ggaaaaattg gcgacggcaa
    21001 aatcttcgtc gctgaattgc aacgcgtcat tcgtattcgt accggcgaag ccgacgaagc
    21061 ggcgctgtaa tctctggcac acagcaacag gaacgaaaaa tgaagatagc gacgataaaa
    21121 actgggcttg cttcactggc gatgctgccg ggactggtaa tggctgcacc tgcggtggcc
    21181 gataaagccg acaatgcgtt tatgatgatt tgtactgcgc tggtgctgtt tatgactatt
    21241 ccggggattg ccctgtttta cggtgggttg attcgcggca aaaacgtgct gtcgatgctg
    21301 acgcaggtga cggtgacgtt tgcactggtc tgtattctct gggtggttta cggttactcg
    21361 ctggcgtttg gtgagggaaa caacttcttc ggcaacatta acgggttgat gctgaaaaac
    21421 atcgaactga cggcggtgat gggcagcatt tatcagtata tccacgtagc gtttcaggga
    21481 tcgtttgcct gcattaccgt aggcttgata gttggagcac tggcggaacg aatccgcttc
    21541 tcagctgtgt tgattttcgt ggtggtatgg ctgacgctct cctacattcc gattgcgcat
    21601 atggtctggg gcggcggttt gctggcttct cacggtgcac tggatttcgc gggtggcacg
    21661 gtggtgcaca ttaacgccgc aatcgccggt ctggtgggcg cgtatctgat aggcaaacgc
    21721 gtgggcttcg gcaaagaggc gtttaaaccg cacaacctgc cgatggtctt caccgggact
    21781 gccattctct atatcggttg gtttggcttt aacgccgggt cagcgggcac ggcgaatgaa
    21841 atcgcagcac tggcatttgt gaatactgtg gtcgcaacgg cggcggcaat tcttggctgg
    21901 atcttcggtg aatgggcgct gcgtggtaag ccttcactgc tgggagcgtg ttcaggcgcg
    21961 attgccggtc tggtcggcgt gacgccagcg tgtggctaca ttggtgttgg cggcgcgttg
    22021 attatcggcg tggtagctgg tctggcgggc ttgtggggcg ttaccatgct caaacgcttg
    22081 ttgcgggtgg atgatccctg cgatgtcttc ggtgtgcacg gcgtttgtgg cattgtcggc
    22141 tgtatcatga ccgggatttt tgctgccagc tcgctgggcg gcgtgggctt cgctgaaggt
    22201 gtgacgatgg gccatcagtt gctggtacag ctggaaagca tcgccattac gatcgtctgg
    22261 tccggtgtgg tggcatttat tggctacaaa ctggcggatc tgacggttgg tctgcgtgtg
    22321 ccggaagaac aagagcgaga agggctggac gtcaacagcc acggcgagaa tgcctataac
    22381 gcgtaacaag cactgcaaaa aacagccgga cggttttcac ctccggctat ttttttaatt
    22441 atgattacgc attacccctt cctgaacggt cgaggcaacc agcacgccgt cctgagtata
    22501 aaactcaccg cgcacaaagc cgcgtgcgct ggacgccgag gtgctctcca cgctatacag
    22561 cagccattca ttcaaattaa acgggcgatg gaaccacatg gaatggtcga tggtcgctat
    22621 ctgaattccc ggttcgagaa aaccgatgcc gtgcggctgt agagctaccg gcaggaagtt
    22681 aagatcagaa gcgtaaccga gcagatactg atgaacgcgc agatcatccg gcacgctacc
    22741 atttgcgcgg atccacacct gacgatgcgg ttctgcgacg tgacctttca atgggttatg
    22801 aaattccacc ggacggactt ccagcggacg atcgcagatg aatttatctt tcagcactgg
    22861 cggcagcagg tgcgccagcg attgggcgat ttgtgtttcc gaaggcaggc catcaggcgc
    22921 tggcgcggac ggcattgttt tttgatgttc gaaacccgct tctggtgcct ggaaagaggc
    22981 cgtcatataa aaaatcggtt tgccgttttg aatagcagca acccggcggg cgctgaagct
    23041 gttaccgtca cgcagcgttt cgacatcata aataatcggc ttcttactat cgcctgggcg
    23101 aagaaagtag ctgtgaaacg aatgtaccag ccgctcttca gggacggtct cttttgcagc
    23161 atacaaggcc tgacccacga cctggccgcc aaacacctgg cgtaaaccta aatcttcact
    23221 ctggccgcga aagagtcctt cctcaatttt ttccagattt aacaatgtca gtaaattttt
    23281 tagcgcctga ctcatataac tctccagtaa caaagctgcc gcagcaagcc aaagtgagtt
    23341 gagtataacg caaatttgcc actggtccga tgggtgcaat ggtctgaatt acgggctaat
    23401 tacaggcaga aatgcgtgat gtgtgccaca cttgttgacg ttactatttt gttaaccact
    23461 cttccggcga ggaaagttag cccgctggtg cattgataat aaggagaagt gaatgaaact
    23521 cgtgcacatg gccagtggtt tagcggttgc gattgcgttg gcggcttgcg cagataaaag
    23581 cgcggatatt cagacgccag caccggctgc aaatacgtct atttcagcaa cacaacaacc
    23641 tgctatccag caaccgaatg tctccggtac cgtctggatc cgtcagaaag tcgcactgcc
    23701 gcctgatgct gtgctgaccg tgacactttc tgacgcgtcg ttagccgatg cgccgtcaaa
    23761 agtgctggcg cagaaagcgg tgcgtaccga aggtaaacag tcaccattca actttgttct
    23821 gccatttaac ccggcagatg ttcagccgaa cgcgcgtatt ctattgagtg cggcgattac
    23881 cgtgaatgac aaactggtat ttattaccga taccgttcag ccggtgatca accagggcgg
    23941 aactaaagcc gacctgacat tggtgccggt gcagcaaacc gccgtgccgg ttcaggccag
    24001 cggtggcgca acggctaccg taccttcgac ttcaccaact caggtgaatc cgtcttcggc
    24061 agttccagct cctacgcaat attaagcgca gttaaccctc tccgcccgga gagggttagt
    24121 agttccagcg ataacgctgc aagtcgattt gcccgcttcc cgataccatc acaccttctg
    24181 ccagtaatgc ctgtcgttga cgctgtaaat ccggtccggt tagcgaaatt gtgccgtggc
    24241 gattaaccac ccggtgccag ggcaaggtgc tgccttcagg gagacgcttt aacacaccgc
    24301 ccacctggcg cgcggcgcgc ggcgatcccg ccagtttcgc cacatcaccg taagtggtga
    24361 catagccttc gggaatagcg gcgacgattt gccagacgcg ttggggaaat gaatcttctt
    24421 tttccatctt ttcttcctga ggtaattttt cagcataatc tggaaaaacg cccgagtgaa
    24481 gccgcattgc gcaagaaacc agcatctggc acgcaatggg ttgcaattag ccggggcagc
    24541 agtgataatg cgcctgcgcg ttggttctca acgctctcaa tgggggctct gttggttctc
    24601 ccgcaacgct actctgttta ccaggtcagg tccggaagga agcagccaag gcagatgacg
    24661 cgtgtgccgg gatgtagctg gcagggcccc cacccatttc tgcctcccac cgtttcgtca
    24721 aaaaatccca acatggctaa actttaacca caactgacgt cgcaagaatt gtctggctgc
    24781 gcagtacgct tcggaggtat gtctgatgaa gtatgttgat ggttttgttg ttgccgttcc
    24841 tgccgataaa aaggatgcct atcgggaaat ggccgctaag gccgcgccat tatttaaaga
    24901 gttcggtgcg cttcgtattg tcgaatgctg ggccagcgat gtaccggatg gtaaagtgac
    24961 cgattttcgt atggcggtga aagcggaaga gaatgaagag gttgtcttta gctggattga
    25021 atacccttca aaagaggtcc gcgacgctgc taatcaaaag atgatgtcgg acccacggat
    25081 gaaagagttc ggcgagtcca tgccgtttga cggcaagcga atgatctatg gtggattcga
    25141 atcaatcatc gacgagtagg tagcgtgacg ggctgtgcgg cgtgccgtca gccctaaaat
    25201 tcacaaatgt tgctcggccc agcgtaagaa atcctctttc ggtaatgcct tgctgtagag
    25261 ccagccctga ccgtagtgca cgccatgctg gcgtaaccac tcttcttgtt tactggtttc
    25321 gattccctcc gctaccattt tcagtttcag tgtttttgcc atttcgatga tatgcggcgt
    25381 gacattttta tattccagcg catcaacgaa agatttatcg atcttcagaa tgtcgacatc
    25441 cagatcctgt aaataactta aacttgaata ccccgtacca aaatcatcga gataaatttc
    25501 atggcccgcc tcccggtagc gagaaattat cggggcgctg gttttcggat cggcaaactc
    25561 gcgttcagtg agttcaagcg cgatctgtct gggattaacc tgatagtgat tgatcatttc
    25621 acgcagcaat tgcgggattt tttccgaggt gagcacggtg gattcaagat tgatcgaaat
    25681 atgctgctgc ggatgctgac gcagccagtc gcccatatct tcgaagacgc ttcttatgat
    25741 cagtagcgtc aacggctcag aaaggcccgt ttgctgtgcc agcggaataa aactatctgg
    25801 tgacaaccaa ctaccgtctg tctgcggcca gcgcgccagt gcctcagcac cgacaatttt
    25861 gccattggct aaggagacaa tcggctgata gtgcacgcaa atatcacgat tttcgatagc
    25921 atcctgcagc cgatgatgcg gtgactggat acggcgcaaa atacgcagca caaacatcgc
    25981 tgccagcagg ccaatcacca acccgagcgg taaccaaata aagacttgcc gatgccagcc
    26041 tttctgcaac attttcgttg aagaccaggt gatgatcgaa atattcattt ccgggaaggg
    26101 caggatatcg taaatgattc cattattttc gatatgctca ccgggtgttt tttgtagtct
    26161 ggtaataatt ccctgagcaa tttcatcgct gctggttatc acaacgttat gggcattgcc
    26221 aataatggcg gcatcaattt gccatgagct atagggaatg acatcaataa aggaagcggg
    26281 gtcgatcatg acgacataat gtgccgttcc catggcaacc atgtaacgga taatgcctaa
    26341 atcgttatgc gatgttaacc agacacgata accatctttc gaaattttac ctggctcggg
    26401 gaaggtatcg ggcgggcttt catgctccag agacgaacac tggggaacgt tgttatcgat
    26461 ataaaccact tcctgaatat agcgataact gtaagatacc cgacgcattt ccatgagatg
    26521 ggcttcgcta caggcagcgc cttgccatct ttccagctct tgcagcgcat ctttcccttg
    26581 tgtcgccacc ttattggccc gaatagcgac gcgggaggaa tacgtattca gctcttcaat
    26641 aaatgatgtt tctacctgct gatgggccag ccagatgctt aagccgacag gcagcaatac
    26701 tgaaagaatc agtactcccg aaataaggcc gaccagatgt cgtgttctca ccagcatatc
    26761 cttattaaca cagtgtaggg aaagtatatc tgattgtgga aaaaggctgg caggagataa
    26821 ggaggtagag ccgggatttc atgaagggat gggtggaaac gaacgttgtc atttgaccag
    26881 gcgcagagct ttacgcctgg tatgtaaccc taacttaaga tgttgattcg gcgcgagcga
    26941 gggtaaaaat atcgtaaaaa gacagttcac ctttacggac gatcattttt tgcagttgtt
    27001 ctttatgaac atccgcgaca cgcggagagt tcaagatggc ttcaaccagc gcggcaggta
    27061 caaagcgcgt gttgtaccat tcgccgttat agcaaacgcg gaaatccagc acgtcgatat
    27121 cttcatagcg gtagcgtggg tagacatcga aaaagaagaa accattaagc aggataaata
    27181 ataccaccag cagccagaca gagcctgaaa gcgtttcgga ctgcaacatc accgccaggg
    27241 tggcaaaaaa agcaacgtac ataccgataa acagccccgg atgtttacgg ataaaactga
    27301 tactaaagcg gggtttgttg tcgcgttttt cgcgggtatt cagagactca atcgtttcgg
    27361 tcagcaggcg ttgtatttcg gtcatcgttt gccttcgtgg tatctgcaac tttccaggga
    27421 acaccctatt ttaacgtggg aatagtagag ggaaagtaac agatttacca caattttgca
    27481 taacagttgc gaaaaatcga ttacaatttt ttaattattc tggctggatt accgcccacg
    27541 acaacgttgt ccgggacatc ttttgtgaca actgcacctg aggctaccac gacgttatca
    27601 ccaatggtca caccagggtt aatgaccgcg cgtccgccaa tccagacgtt attaccgatg
    27661 gtgacgggtt tccccagctc agcaccgcta ttacgtgcta cagggtcgat gggatgtgtt
    27721 gccgtgtaga tatgaacgcc tggtgccaac atacagttat caccgatgcg aatagggcag
    27781 acatcaagca tcacgcaatc gaagttggcg aaaaaattat tgccgagaaa aatgttatag
    27841 ccataatcac agcgaaacgt tggctcaata taagcctctg tcacctgacc gaataaatca
    27901 gcgagaattt gctggcgtaa tgtgtgctct tccgccaggg aatgattgta tcggtgaata
    27961 agctgacgag cgcgcaggcg atcgcgagat aacgtctcat ctgctgagcg atacaactca
    28021 ccagcaatca tcttttcttt ttctgtgctc attactgacc tccggttgct gaaaatgcgg
    28081 caacacagta agacgaaaag tcagtgaaga gaacgttccc attacatctg tgtgataaag
    28141 atcacatagg gtatgtcttc atggcgaaaa agtataaaat tcttaataaa cagccggtta
    28201 tagctccgaa agcgaattat ttattagcga ataaatttcc atactgagga agggatcttg
    28261 tcgtacagtt tattcatggt caattcggcg aggcggtgat ctgcggctga gtaaaatacc
    28321 gccagttcat tatctgataa ttcgtattta tttttctcga taacacgctc cagcgtgtca
    28381 attgtctggc aacgacgtaa acgcattaaa taatcggttt ttgttaaagg tttttcggac
    28441 atacttctac ccatggttgt aataattcta acaagataaa ctcgcaggat tctctttcgt
    28501 cgcattgaca aaacaacgga atagtcgatt acctgacttc cgccatttct gaagatcctg
    28561 catattaata ccataactgc tgaacaacat aaaggtgtca tccagatatt cgtcgatctg
    28621 ctcaatgagc ttattgtctt cattatactt aattttgtaa ttaagtgcga aggtcgcaat
    28681 atgctcaatc agttcattca actggaggtt gatcgccgag gttgggtcgt taacccagcc
    28741 atgattgctt tcttcaaggt ttgcaagaca gtcatgatac agggtttcac agagaaactt
    28801 aagctgcgcg atatcatgtc ttttgggtga gtattcatcc ataacgcgtc cccttcttag
    28861 cggttgaact aacggacacc tttcgggatg gaaaaaactt actgacctgg acttgccctc
    28921 tcgtttgtta gcttaactat aagccactct ttgcaggttt tcatcgcatt attacgaaaa
    28981 attacaatta ggaaggaagc gggacaggac tcactttttc atactagcac taacccgcag
    29041 caggtaaaag cagtttgctc gaactgttca taacatttca ggattatcgc cagatgaaat
    29101 tggtaacact aataccagga ttgctctgaa tatgacgtaa taaccgagga atgaataaag
    29161 aattaccgca aat
//
LOCUS       DAPHCV010000062        28845 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.062, whole genome shotgun sequence.
ACCESSION   DAPHCV010000062 DAPHCV010000000
VERSION     DAPHCV010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.062"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            177..1154
                     /gene="csiD"
                     /locus_tag="RH033_002660"
     CDS             177..1154
                     /gene="csiD"
                     /locus_tag="RH033_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HDV2991826.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGV
                     DDVKQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATNHYQTHQ"
     gene            1174..2442
                     /gene="lhgO"
                     /locus_tag="RH033_002661"
     CDS             1174..2442
                     /gene="lhgO"
                     /locus_tag="RH033_002661"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HDV2991827.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2465..3913
                     /gene="gabD"
                     /locus_tag="RH033_002662"
     CDS             2465..3913
                     /gene="gabD"
                     /locus_tag="RH033_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDV2991828.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERATILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGVPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLENGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            3927..5207
                     /gene="gabT"
                     /locus_tag="RH033_002663"
     CDS             3927..5207
                     /gene="gabT"
                     /locus_tag="RH033_002663"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HDV2991829.1"
                     /translation="MSSNKELMQRRSQAVPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMDVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGHKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            5445..6845
                     /gene="gabP"
                     /locus_tag="RH033_002664"
     CDS             5445..6845
                     /gene="gabP"
                     /locus_tag="RH033_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HDV2991830.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYADVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVLSTGLLAIGIICTVPIMAR
                     WKKLIMWQKTPIHNTR"
     gene            6866..7528
                     /gene="csiR"
                     /locus_tag="RH033_002665"
     CDS             6866..7528
                     /gene="csiR"
                     /locus_tag="RH033_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HDV2991831.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSYYLLQMRERLFDLATR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTATVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7529..7978)
                     /gene="kbp"
                     /locus_tag="RH033_002666"
     CDS             complement(7529..7978)
                     /gene="kbp"
                     /locus_tag="RH033_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HDV2991832.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8062..8220)
                     /locus_tag="RH033_002667"
     CDS             complement(8062..8220)
                     /locus_tag="RH033_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HDV2991833.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8403..8702
                     /locus_tag="RH033_002668"
     CDS             8403..8702
                     /locus_tag="RH033_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV2991834.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            8712..9236
                     /gene="ygaP"
                     /locus_tag="RH033_002669"
     CDS             8712..9236
                     /gene="ygaP"
                     /locus_tag="RH033_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HDV2991835.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLKQSGLPAKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVILGYTVNSGFFLLSGFVGAGLMFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(9283..9687)
                     /gene="stpA"
                     /locus_tag="RH033_002670"
     CDS             complement(9283..9687)
                     /gene="stpA"
                     /locus_tag="RH033_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HDV2991836.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     QQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFIDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            10354..10803
                     /gene="alaE"
                     /locus_tag="RH033_002671"
     CDS             10354..10803
                     /gene="alaE"
                     /locus_tag="RH033_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HDV2991837.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADIMAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(10840..11184)
                     /locus_tag="RH033_002672"
     CDS             complement(10840..11184)
                     /locus_tag="RH033_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HDV2991838.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            11336..11665
                     /locus_tag="RH033_002673"
     CDS             11336..11665
                     /locus_tag="RH033_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HDV2991839.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            11913..12158
                     /gene="nrdH"
                     /locus_tag="RH033_002674"
     CDS             11913..12158
                     /gene="nrdH"
                     /locus_tag="RH033_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDV2991840.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            12155..12565
                     /gene="nrdI"
                     /locus_tag="RH033_002675"
     CDS             12155..12565
                     /gene="nrdI"
                     /locus_tag="RH033_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDV2991841.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            12538..14682
                     /gene="nrdE"
                     /locus_tag="RH033_002676"
     CDS             12538..14682
                     /gene="nrdE"
                     /locus_tag="RH033_002676"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDV2991842.1"
                     /translation="MATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MTTHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHASGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDIERVYGKPFADVAISEHYAEL
                     VANERIRKKYINARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFTRSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            14692..15651
                     /gene="nrdF"
                     /locus_tag="RH033_002677"
     CDS             14692..15651
                     /gene="nrdF"
                     /locus_tag="RH033_002677"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDV2991843.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIHLIIRDEAVHGYYIGYKYQKNLEKISQARREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            16007..17209
                     /gene="proV"
                     /locus_tag="RH033_002678"
     CDS             16007..17209
                     /gene="proV"
                     /locus_tag="RH033_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HDV2991844.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKAALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            17202..18266
                     /gene="proW"
                     /locus_tag="RH033_002679"
     CDS             17202..18266
                     /gene="proW"
                     /locus_tag="RH033_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HDV2991845.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPAAAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            18324..19316
                     /gene="proX"
                     /locus_tag="RH033_002680"
     CDS             18324..19316
                     /gene="proX"
                     /locus_tag="RH033_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HDV2991846.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYGLTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            19508..20692
                     /locus_tag="RH033_002681"
     CDS             19508..20692
                     /locus_tag="RH033_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2991847.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAACGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMTLALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            20816..21553
                     /gene="ygaZ"
                     /locus_tag="RH033_002682"
     CDS             20816..21553
                     /gene="ygaZ"
                     /locus_tag="RH033_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HDV2991848.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            21543..21878
                     /gene="ygaH"
                     /locus_tag="RH033_002683"
     CDS             21543..21878
                     /gene="ygaH"
                     /locus_tag="RH033_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HDV2991849.1"
                     /translation="MSYEVLLLGLLVGTANYCFRYLPLRLRVGNARPTKRGAIGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            21969..22499
                     /gene="emrR"
                     /locus_tag="RH033_002684"
     CDS             21969..22499
                     /gene="emrR"
                     /locus_tag="RH033_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HDV2991850.1"
                     /translation="MDSSFTPIEQMLNFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            22626..23798
                     /gene="emrA"
                     /locus_tag="RH033_002685"
     CDS             22626..23798
                     /gene="emrA"
                     /locus_tag="RH033_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HDV2991851.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VTPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            23815..25353
                     /gene="emrB"
                     /locus_tag="RH033_002686"
     CDS             23815..25353
                     /gene="emrB"
                     /locus_tag="RH033_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HDV2991852.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(25417..25932)
                     /gene="luxS"
                     /locus_tag="RH033_002687"
     CDS             complement(25417..25932)
                     /gene="luxS"
                     /locus_tag="RH033_002687"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDV2991853.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARNILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(26082..27638)
                     /gene="gshA"
                     /locus_tag="RH033_002688"
     CDS             complement(26082..27638)
                     /gene="gshA"
                     /locus_tag="RH033_002688"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HDV2991854.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(27711..28139)
                     /locus_tag="RH033_002689"
     CDS             complement(27711..28139)
                     /locus_tag="RH033_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV2991855.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(28136..28702)
                     /gene="yqaB"
                     /locus_tag="RH033_002690"
     CDS             complement(28136..28702)
                     /gene="yqaB"
                     /locus_tag="RH033_002690"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HDV2991856.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         6829 a         7440 c         7453 g         7123 t
ORIGIN      
        1 ttcaacccaa ttcttatccc tcacattatt tgttattaat ttgttgtttt tgatcgcaat
       61 aagaaaacaa tatgtaactt ttatgcgcat ttttcagaaa tgtagatatt tttagattat
      121 ggctacgaaa tgagcatcgc catatcaccc tacatctcat aagaggatcg cttctgatga
      181 atgcactgac cgccgtacaa aataacgctg tcgattcagg ccaggactat agcggattca
      241 ctctcatccc gtcggcgcaa tccccgcgtc tgctggaact caccttcacc gaacagacga
      301 ccaaacagtt tctcgaacag gttgccgaat ggcccgtgca ggcgctggag tacaaatctt
      361 ttctgcgttt tcgggtaggc aaaattcttg acgatctgtg tgcgaaccag ctgcaaccat
      421 tgctgttgaa gaccctgcta aaccgtgctg aaggtgcgct gttgatcaat gcggtgggtg
      481 tcgatgatgt gaagcaagcg gatgagatgg tgaagctggc gacggcggta gcgcatctga
      541 ttggccgttc caatttcgac gcgatgagcg gtcagtatta cgcgcgtttt gtagtgaaaa
      601 atgtcgataa ctcagacagc tatctgcgtc agccgcaccg cgtaatggag ctgcataacg
      661 acggcaccta cgtcgaagag atcactgact atgtgctgat gatgaaaatc gacgagcaga
      721 acatgcaggg tggaaattcg ttgctgctgc atctcgatga ctgggaacat ctggaccact
      781 atttccgcca tccactggcg cgtcgcccga tgcgctttgc cgcgccgccg agcaaaaacg
      841 tcagcaaaga tgttttccat ccagtgttcg acgttgatca acagggtcgc ccggtgatgc
      901 gttatatcga ccagttcgtc cagccaaaag acttcgaaga aggcgtgtgg ctgagtgagc
      961 tttccgatgc cattgaaacc agcaaaggca ttctttctgt gcccgttccc gttggcaaat
     1021 tcctgttgat taacaatcta ttctggctgc acggtcgcga ccgctttact ccacacccgg
     1081 atctgcgccg tgaactgatg cgtcagcgtg gctatttcgc ttacgccact aaccactacc
     1141 agacgcatca gtaagcgcaa aggaattgag cggatgtatg attttgtgat tattggcggc
     1201 ggcatcatcg gcatgtcgac cgccatgcaa ctgattgatg tttatccgga tgcccgcatt
     1261 gcgttgctgg aaaaagagtc cggtccggcc tgtcatcaga caggccacaa cagcggcgtg
     1321 atccatgccg gggtttatta cacgcccggt agcctgaagg cgcagttttg cctggcgggg
     1381 aaccgcgcca ccaaagcctt ttgcgatcaa aacggcattc gctatgacaa ctgcggcaag
     1441 atgctggtcg ccacctctga actcgaaatg gaacggatgc gcgcgttgtg ggaacgtacg
     1501 gcggcgaatg gtatcgagcg cgagtggcta aacgccgagg aactgcgcga gcgcgaaccg
     1561 aatatcaccg ggctcggcgg catttttgtg ccgtccagcg gcattgtcag ctatcgcgaa
     1621 gtgacggcgg cgatggcaaa aatcttccag gccagaggcg gcgagattat ttataacgcc
     1681 gaagtcagcg ccctcagtga gcataaaaac ggcgtggtga tacgtacccg tcagggcggt
     1741 gaatatgaag catcgacgct gattagctgt tccgggctga tggctgaccg gctggtgaaa
     1801 atgctcggcc tcgaaccggg ctttattatc tgcccgttcc gtggcgagta tttccgcctt
     1861 gcgccggagc ataaccagat tgttaaccac ctgatttacc ccattcccga ccccgcgatg
     1921 ccatttttgg gcgtgcatct cacccgcatg atcgatggca gcgtgaccgt cgggccaaac
     1981 gcggtgctgg ctttcaaacg cgaaggctat cgtaagcgcg acttctcatt tagcgacacg
     2041 ctggagattt tgggctcgtc ggggattcgc cgggtgctgc aaaaccatct acgctcagga
     2101 ctgggcgaga tgaaaaactc gctgtgcaaa agcggctatc tgcggctggt gcaaaagtat
     2161 tgtccccggc tttcgttaag cgatctccag ccctggcccg ctggtgtgcg ggcgcaggcg
     2221 gtatcgccgg acggcaagct gattgacgat tttctgtttg tcaccacccc gcgcacgatc
     2281 cacacctgca atgcgccctc cccggcagcg acatcagcaa ttcctattgg tgcgcatatt
     2341 gtcagcaagg tacaaacgct gttggcaagc cagagtaacc ccggacgcac gctgcgagcg
     2401 gcacgtagtg tggatgcctt acacgccgca tttaatcaat aacctttgaa aacaggacgt
     2461 aacaatgaaa cttaacgaca gtaacttatt ccgccagcag gcgttgatta acggggaatg
     2521 gctggacgcc aacaatggcg aggtcatcga cgtcaccaat ccggcgaacg gcgacaagct
     2581 gggtagcgtg ccgaaaatgg gcgctgatga aacccgcgcc gctatcgacg ccgccaaccg
     2641 cgctctgccc gcctggcgcg cgctcaccgc caaagaacgc gccaccattc tgcgcaactg
     2701 gttcaatttg atgatggagc atcaggacga tttagcgcgc ctgatgaccc tcgaacaggg
     2761 taaaccgctg gctgaagcga aaggcgaaat cagctacgcc gcctccttta ttgagtggtt
     2821 tgctgaagaa ggcaaacgta tttatggcga caccattccc ggtcatcagg ccgataaacg
     2881 cctgattgtt atcaagcagc cgattggcgt cactgctgct atcacgccgt ggaactttcc
     2941 ggcggcgatg attacccgta aagccggtcc ggcgctggcg gcaggctgca cgatggtgct
     3001 gaaacctgcc agtcagacgc cgttctctgc gctggcgctg gcggaactgg cgattcgcgc
     3061 aggcgttccg gctggcgtat ttaacgtggt caccggttcg gcgggcgcgg tcggtaacga
     3121 actgaccagc aacccgctgg tgcgcaaact gtcgtttacc ggctcgaccg aaattggccg
     3181 ccagttaatg gaacagtgcg cgaaagacat caagaaagtg tcgctggaac tgggcggcaa
     3241 cgcgccgttt atcgtctttg acgatgccga cctcgacaaa gccgtggaag gcgcactggc
     3301 ctcgaaattc cgcaacgccg ggcaaacctg cgtctgcgcc aaccgtttat acgtgcagga
     3361 cggcgtgtat gaccgctttg ccgaaaaatt gcagcaggcg gtgagcaaac tgcacatcgg
     3421 cgacgggctg gagaatggcg tcaccattgg gccgctgatt gatgaaaaag cggtagcaaa
     3481 agtggaagag catattgccg atgcgctgga gaaaggtgcg cgtgtggttt gcggcggtaa
     3541 agcggatgaa cgcggcggca acttcttcca gccgaccatt ctggtggacg ttccggccaa
     3601 cgccaaagtg tcgaaagaag agacgttcgg cccccttgcc ccgctattcc gctttaaaga
     3661 tgaagccgat gtgattgcgc aggccaatga caccgagttt ggccttgccg cctatttcta
     3721 cgcccgtgat ttaagccgcg tcttccgcgt gggcgaggcg ctggagtacg gcatcgtcgg
     3781 cattaatact ggcattattt ccaatgaagt ggccccgttc ggcggcatta aagcctcggg
     3841 tctgggtcgt gaaggttcga agtatggcat cgaagattac ttagaaatca aatatatgtg
     3901 catcggtctt taactggaga acgcgaatga gcagcaataa agagttaatg cagcgccgta
     3961 gtcaggcagt tcctcgtggc gttgggcaaa ttcaccccat tttcgctgac cgcgcggaaa
     4021 actgccgggt gtgggacgtt gaaggtcgtg agtatcttga tttcgcgggc ggcattgcgg
     4081 tgctcaatac cgggcacctg catccgaaag tggttgcggc ggtggaagcg cagttgaaaa
     4141 agctgtcgca cacctgcttc caggtgctgg cttacgaacc gtatctggag ctgtgcgaga
     4201 ttatgaatca gaaggtgccg ggagatttcg ccaaaaaaac gctgctggtt acgaccggtt
     4261 ccgaagcggt ggaaaacgcg gtgaaaatcg cccgcgccgc cactaaacgt agcggtacta
     4321 tcgcctttag cggtgcgtat cacgggcgca cgcattacac gctggcgctg accggcaagg
     4381 tgaatccgta ctctgcgggc atggggctga tgccgggtca tgtttatcgc gcgctttatc
     4441 cttgcccgct gcacggcata agcgaggatg acgctatcgc cagcatccac cggatcttca
     4501 aaaatgacgc cgcgccggaa gatatcgccg ccatcgtgat tgagccggtt cagggcgaag
     4561 gcggtttcta cgccgcgacg ccagccttta tgcagcgttt acgcgcgctg tgtgacgagc
     4621 acgggatcat gctgattgcc gatgaagtgc agagcggcgc ggggcgtacc ggcacgctgt
     4681 ttgcgatgga gcagatggac gttgcgccgg atcttaccac ctttgcgaaa tcgatcgcgg
     4741 gcggcttccc gctggcgggc gtcaccgggc gcgcggaagt gatggatgcc gtcgctccag
     4801 gcggtctggg cggcacctat gccggtaacc cgattgcctg cgtggcagcg ctggaagtgt
     4861 taaaggtatt cgagcaggaa aatctgctgc aaaaagccaa cgacctgggg cataagctga
     4921 aagacggatt gctggcgata gccgaaaaac acccggagat cggcgatgta cgcgggctgg
     4981 gagcgatgat tgccatcgag ctgtttgaag atggcgatca cagcaaaccg gacgccaaac
     5041 tcaccgccga gatcgtggcg cgtgcccgcg ataaaggcct gattcttctc tcctgcggcc
     5101 cgtattacaa cgtgctgcgc atccttgtac cgctcaccat tgaagacgct cagatccgtc
     5161 agggtctgga aatcatcagc cagtgttttg ctgaggcgaa gcagtagatc cgtacccatg
     5221 ccggaggcga cgctgcgcgt cttgtccggc ctacgcgaat ccaggtcgga taaagcgttt
     5281 acgctgcatc cgacaatctg tacgtgaaca ggaataaatc tatgtcggcc ggataaggcg
     5341 gagccgctct ccggtaaaaa gaaccaataa caattacacg cgtgacccga cgcgggaaat
     5401 gtcggggcgc tctcccaagt gacacacttt cgagaggatt caggatgggg caatcatcgc
     5461 aaccacatga gttaggcggc gggctgaagt cacgccacgt caccatgttg tctattgccg
     5521 gtgttatcgg cgcaagtctg tttgtcggtt ccagcgtcgc catcgccgaa gcgggcccgg
     5581 cggtattact ggcctatctg ttcgccggat tactggtggt tatgattatg cggatgttgg
     5641 cggaaatggc agttgccacg cccgataccg gttcgttttc cacctatgcc gataaagcta
     5701 ttggccgttg ggcgggctat accatcggct ggctgtactg gtggttttgg gtactggtta
     5761 tcccgctgga agccaacatc gccgccatga tcctgcactc gtgggttcca ggcattccca
     5821 tctggttatt ttccctcgtc attaccctcg ccttaaccgg cagtaattta ttaagcgtta
     5881 aaaactacgg cgaatttgag ttctggctgg cgctgtgtaa agtcatcgct atcctggcct
     5941 ttattttcct tggtgcagtc gcaattagcg gtttttaccc gtatgccgac gtgagcggta
     6001 tttcaagatt gtgggatagc ggcggcttta tgcccaacgg cttcggtgcg gtattaagcg
     6061 cgatgttgat caccatgttc tcgtttatgg gcgcagaaat tgtcaccatt gccgccgcgg
     6121 aatccgacac gccggaaaaa catattgtcc gcgccaccaa ctcggttatc tggcgtatct
     6181 ctattttcta tttgtgttct atttttgtcg ttgtggcatt aataccgtgg aatatgccag
     6241 ggctgaaagc cgttggttct tatcgctcgg ttctggaatt gctcaatatt cctcatgcga
     6301 aattaatcat ggactgcgtg atattacttt ccgtaaccag ttgcctgaac tcggcgctgt
     6361 ataccgcttc caggatgctc tactccttaa gccgtcgcgg tgatgctccc gcggtaatgg
     6421 gtaaaatcaa ccgcagtaaa accccgtacg tagcggtgtt actctccacc ggcgcggcat
     6481 tcttaacggt ggtggtgaac tattacgcgc ctgcgaaggt gtttaaattt ctgatcgaca
     6541 gctccggcgc tatcgccctg ctggtttatt tagtcatcgc cgtttcacaa ttgcggatgc
     6601 gcaaaattct gcgagcagaa ggaagcgaaa ttcgcctgcg gatgtggctt tatccgtggc
     6661 tcacctggct ggtcatcggt tttattacct ttgtgttggt agtgatgcta ttccgcccgg
     6721 cgcaacagtt agaagtgctc tccaccggct tattagcgat tgggattatc tgtaccgtgc
     6781 caattatggc tcgctggaaa aagctgataa tgtggcaaaa aacacccatt cataatacgc
     6841 gctgataatg atcaggagtc acaccatgac cattacctct ctggatggct atcgctggct
     6901 gaagaacgat attattcgcg gcaattttca gccggatgaa aaattacgga tgagtttgct
     6961 gacatcgcgt tatgcacttg gcgtcggacc gttgcgggag gcgctttcgc aactggtggc
     7021 agaacgactg gtcacggtgg tgaatcaaaa agggtatcgg gtggcgtcta tgtcagagca
     7081 ggagctgctc gatattttcg acgcccgcgc caatatggaa gcgatgctgg tgagtctggc
     7141 gattgcccgc ggtggcgatg aatgggaggc agacgttctt gcgaaggcgc atctgctcag
     7201 taagcttgag gcctgtgacg ccagtgagaa aatgcttgat gagtgggatc tgcgtcatca
     7261 ggcgtttcat actgcaattg tggcgggctg tggttcttac tatttgctgc aaatgcgtga
     7321 acggttgttt gatctggcaa cgcgttatcg atttatctgg ctgcggcgaa cagtgctttc
     7381 ggtggaaatg ctggaagata aacacgatca gcaccagacc ctgactgcga cggtactggc
     7441 gcgagatacc gcgcgcgcca gtgagttaat gcgccagcat ttactgacgc caattcccat
     7501 tatccagcag gcgatggctg gcaattaact actcttcagg aatacgcaac acttgccccg
     7561 ggtaaatttt atccgggctt tttagcatcg gtttattcgc ttcgaagatt ttattgtaca
     7621 gattagcgtt accgtagacc tgtttggaaa tggcactcag agtgtcgcca gacttaacgg
     7681 tataaaactg gctggcagtg gctggtgtcg ccgttttcac ctgatcatcg acactggcaa
     7741 taccggaaat attccccacc gcaacaagga ttttctcctt cgcctcctga cttaagccgt
     7801 cacccgtgac cgtcgctttg ccgtcggcaa tttgaatgtt cactttatcg gcatcaggta
     7861 taccggtttt gttcagatgc tcctgcacct tcttcgcctg atcgtcttta tcgtgctgac
     7921 ctgtaaccgc gtcccagagt ttttctccgg catctttcac aaaattgaac agacccatag
     7981 ctacctcatt cgttaacgga aacactcaga aagtgtagca gaggaacgcg gcgcgttaac
     8041 gccgcggggg tgaacacagg tttaatcgcg ggtttgcacc cagaacgcgt gaatcaaacc
     8101 ggggatatac ccaagcagtg tcagcagaat attaataatg aacgcccaac cgaacccttt
     8161 accgagcagc acgccgagcg gcggcagaat gatggtgatg acgattctcc agaaacccat
     8221 atgtactccc tataagaaaa ttactcattg tttaaaaaga gattttatct cttaagcgta
     8281 gtaattttag cggagcctgc cagtttcagc ttacgctttt actctcattt acgctgctga
     8341 ggctggtata tgttgcattt ttaatttaga gtttacttaa tttagaaaat acttaaatat
     8401 ctatgactga actcgcgcaa ttacaggcta gtgccgaaca ggcagcggcc ttattgaaag
     8461 caatgagcca ccctaaacgg ttgctgattc tgtgcatgct tagcggttcc cccggcacca
     8521 gtgcgggaga gctgacgcgc attaccggac taagtgcctc tgcaacatca cagcatctcg
     8581 ctcgtatgcg ggacgaaggg cttatcgaca gccaacggga tgcccaacgc attctatatt
     8641 ccattaaaaa tgaggcggta aatgccatta tcgccaccct gaaaaatgtc tattgtccgt
     8701 aaggagtcac tatggctttg acaaccattt cgccgcatga tgcacaagaa ttaatcgcac
     8761 gcggcgcaaa gttaatcgat attcgtgacg ctgatgaata tcttcgtgaa catattcctg
     8821 aagcagatct ggctccatta tccgtgctga aacagtcagg tcttccggct aaattacgtc
     8881 gcgagcaaat aatattccac tgccaggcag gtaaacgcac cagtaataac gccgataaat
     8941 tagcagcgat tgccgccccc gcagaaatct ttttactcga agatgggatt gatggctgga
     9001 aaaaagcagg attaccagta gcggtaaata aatctcaacc cttgccgtta atgcgccagg
     9061 tgcagatcgc tgcgggtggt ttaatattaa tcggcgttat actaggttat accgtaaata
     9121 gcggtttctt cttattaagc ggctttgttg gtgccgggtt aatgtttgca ggaattagcg
     9181 gtttttgtgg gatggcaagg ttgttagata agatgccgtg gaaccaacga gcttgagaag
     9241 cgacgccgga cgcgccatag cagcaacatc cggcctcagt aattagatca ggaaatcgtc
     9301 gagagattta ccttctgcca gcgcctgagc gattggcttc ggtgtacggc cctgaccggt
     9361 ccaggtttta gtttcaccgt taacatcgat gaatttatat ttcgccggac gcggctggcg
     9421 ttttttacca gcgcgtggtg ccgcagcaga gctattaccc aataactctt ccgggttaat
     9481 tccgtcagct ttcatcagct ccagccaggt gctaattttt tcctggcgct ctgccagttc
     9541 acgctgctgt tgttgttctt cttcacgtct ttctttagtg acaaccctga atttttcgag
     9601 catttcttca agaacgtcaa tggagaattc gcgagccatc gcacggaggg tgcgaatgtt
     9661 atttaaactt tgtaacatta cggacataat aaagaaaacc ttttaacgcc aaaacaaaaa
     9721 agtatttcgt cgcgtagtgt aagcgatcac ttattatatt tccacaaaat ttaatctatt
     9781 tacagtcctg cacgagatta tttcagctgt tattttctga aaccctcaga ataatttcaa
     9841 gattatatat tagccatttt gatgagtaat ttaggttatt ggtgaacaga gcggcgctct
     9901 ctgctcgcta attccgcaag cccgacaaaa ccaaagtgaa taaaaatgca actcaacaga
     9961 ataaaagaaa acatctatca ctggtgcttt ctgctgtcaa tgtacattat ctggcataaa
    10021 aaatgctgaa aatgacccga tcactacata caccgaattc tctacgctta tagcgttacc
    10081 cccccccaaa ccttcatgag tgaaaataaa ggtaaacaca tatccaattg atttaattaa
    10141 aaataaatca tatcggcatg taaaaatcca caatgtgcaa aaaacgcgcc gctacggaat
    10201 attttatcta caaaaacaga ttaaataaaa atttttcact aattgattag tcatagccag
    10261 cgatatacgc tatgcaaaaa tgcagatggc aatgagatcc actgctttca tctccattca
    10321 catcccatta cgcttttatt aaggagcatt agcatgttct caccgcagtc acgcttgcgt
    10381 catgcagttg cagatacgtt cgcgatggtt gtttactgtt ctgtcgtgaa catgtgtatt
    10441 gaagttttcc tctccggaat gagcttcgaa cagtcttttt attccagatt ggtagcgatt
    10501 ccggtaaaca tcttaattgc atggccatac ggtatgtacc gcgatctgtt tatgcgcgca
    10561 gcacgcaaag ttagcccgtc gggctggata aaaaatctgg cggatatcat ggcttatgtg
    10621 acgttccagt caccggtgta tgtggcgatc ttgttagtgg tgggcgcaga ctggcatcag
    10681 attatggcgg cggtcagttc aaacatcgtt gtttcgatgt tgatgggggc ggtttatggc
    10741 tacttcctcg attattgccg ccgactgttt aaagtcagcc gttaccagca ggtaaaagcc
    10801 tgacccttct tttcgcgact ggcatcgcca gtcgcgtctt taactggctt cgccaaataa
    10861 accattcaaa taacgttcaa gcgcaacacg cgaactaaag ccatgcggga taccgtaatg
    10921 ctcgtgtcgc tcgcctgcta aatagagcgg aaattgttga taatgatcgc aagttttaat
    10981 atcggaagcg ggttcagcca gtgttgaact acgttctttt aatgttggat gaatcaccag
    11041 ggcggtacgt cccattcgcg cttcgcgatt aacatagaca taattttccc cacggcggta
    11101 accataagcc ttctgggtta ccacatcgac agtaaaaccg actttttcaa gtacgcgcgc
    11161 cacctcgtct ggtcgtaaat acatatttga tcctcgttat tatcaaccgc gggcctacct
    11221 tacctgattg cgcattcaca acgctttcag aaaagtccat aaagctgcga tgaacctcag
    11281 ttaagaaaat tatggtctac actgaaaatt acatcgaatt ctaatggggg atcatatgtt
    11341 taaccgaccg aaccgcaacg acgttgatga tggcgtgcag gatatccaga atgatgtcaa
    11401 tcaattagct gatagtctcg aatctgtatt gaagtcctgg ggcagcgacg ccaaagggga
    11461 agctgaagcc gcacgcagta aagcgcaggc tctactgaaa gaaaccaggg cgcgaatgca
    11521 tggtcgtact cgcgtccagc aagccgcgcg tgatgccgtt ggctgcgcgg attcttttgt
    11581 tcgtgaaaga ccctggtgta gcgtgggtac agcagctgcg gtaggtattt ttatcggcgc
    11641 actgttaagc atgcgcaaat cgtagtgcaa taatgataat aaatacgcgt ctttgacccc
    11701 gaagcctggc ttcggggttt ctttttgcct ggtgaatcac aaaaatcccc ctaccccgtc
    11761 acgcttatac ccagggtaat ttcgaccact atttgctata tattgtatgg ttgaatcttt
    11821 tttcaactac atctagtatc tctgtatcaa cagagagaca acccgacgcg tatcatcgcg
    11881 ccgtatcttc attttaaacg gaaatacgaa tcatgcgcat tactatttac actcgtaacg
    11941 attgcgttca gtgccacgcc accaaacggg cgatggaaaa ccggggcttt gactttgaaa
    12001 tgattaatgt cgatcgcgtt cctgaagcgg cggaagcgct gcgtgctcag ggctttcgtc
    12061 agttgccggt tgtgattgca ggcgatctta gctggtctgg tttccgcccg gacatgatca
    12121 atcgtctgca tccagcgcca cacgctgcca gtgcatgagc cagctcgtct acttttccag
    12181 cagctccgaa aatacgcagc gttttatcga acgtttaggg ctgcccgcgg tgcgcatccc
    12241 gctcaatgag cgggaacgga ttcaggtaga cgagccttac atcctgatcg tgccctctta
    12301 cggcggcggc ggtacggctg gcgcggtgcc acgacaggtc attcgctttt taaacgacga
    12361 gcataaccgg gcgttgctgc gcggcgttat tgcttcgggt aatcgcaact ttggtgaggc
    12421 atatggccgc gccggagatg tgattgcccg gaaatgcggc gtgccatggc tgtaccgctt
    12481 tgaactcatg ggtacgcaaa gcgatatcga aaacgttcgt aaaggagtaa ccgaattttg
    12541 gcaacgacaa ccgcagaacg cctgacgcag gaaactatgg attaccacgc gctgaatgcg
    12601 atgcttaacc tttacgatag cgcaggtcgc attcagttcg ataaagatcg ccaggcagta
    12661 gacgccttta tgacgacgca tgtgcgtccg aacagtgtga ccttcagtag ccaacagcag
    12721 cgcctgaact ggctggtcaa cgaaggttac tatgatgaaa gcgttcttaa tcggtactct
    12781 cgcgactttg tcattacgct gtttgcccac gcacacgcca gcggttttcg ttttcagaca
    12841 ttcctcggag catggaagtt ttacaccagc tatacgttga agacattcga cggtaaacgt
    12901 tatctggaag attttgccga tcgagtcacg atggtggcgc tgacgctggc acaaggcgat
    12961 gaaacactgg cgacgcaact gaccgatgag atgctgtcag gacgctttca gcctgcaaca
    13021 ccgacattcc tcaactgtgg taagcagcag cgcggcgaac tggtttcctg ttttttgctg
    13081 cgtattgaag acaatatgga gtcgattggc cgggcagtaa attccgcact gcaactgtcg
    13141 aaacgcggcg gcggcgttgc atttttgctg tcgaatctgc gggaagcggg cgcgccaatt
    13201 aaacgtattg aaaatcaatc ttctggcgtg attccggtga tgaaaatgct ggaagacgca
    13261 ttttcctatg ccaaccagct cggcgcgcgt cagggtgctg gcgcagtcta tttacatgcc
    13321 catcatcccg atattctgcg ttttctcgat acgaaacggg aaaatgccga cgagaaaatc
    13381 cgcattaaaa cactgtcgct gggcgtggtg atcccggata tcactttcca tctggcaaaa
    13441 gagaatgcgc agatggcgct gttttcgccc tatgacatag agcgagttta tggcaagcca
    13501 tttgccgatg tcgccatcag tgaacactat gccgaactgg ttgccaatga acgcatccgc
    13561 aaaaaataca tcaacgcccg tgatttcttc cagcgactgg cagaaatcca gtttgagtcc
    13621 ggctatccct acatcatgta tgaagacacg gttaaccgtg ctaaccctat cgccgggcgc
    13681 ataaatatga gcaatctctg ctcagaaatt ttgcaggtta acagcgcctc agagtatgac
    13741 gagaatctcg actatgcccg cacaggccat gatatttcct gcaatttagg ttcgttgaat
    13801 attgcccaca ccatggattc ccccgacttt gcccgcacgg tagagactgc cgtgcgcggt
    13861 ttaacggcgg tgtcagatat gagtcatatc cgcagcgtgc cgtccatcga agccggaaat
    13921 gccgcctcac acgccatcgg actggggcag atgaatttac acggttatct ggcgcgagaa
    13981 ggcatcgctt atggatcgcc ggaagcactc gatttcacca atctctattt ctataccatc
    14041 acctggcacg cactgcgtac ctcgatgttg ctggcgcgcg aacgcggtga aaccttcgcc
    14101 gggttcaaac agtcacgcta tgccagtggt gaatatttta gccaatatct gcaagggaac
    14161 tggcagccga aaacggcgaa agttggcgaa ctgtttaccc gtagcggtat tacgttacct
    14221 acccgtgaga tgtgggcgca gctgcgcgac gacgtgatgc gctacggcat ttacaaccag
    14281 aatcttcagg cggtgccgcc aaccggttct atctcttata tcaaccatgc tacgtcgagt
    14341 attcatccga ttgtggcgaa agtagagata cgcaaagagg gcaaaacagg acgcgtttac
    14401 taccctgccc cgtttatgac taacgagaat ctggcgctgt atcaggacgc ttacgaaatt
    14461 ggcgcagaaa agatcatcga cacctacgcg gaagcgactc gccatgtcga tcaggggctg
    14521 tcgctgacgc tgtttttccc cgataccgcc accactcgcg atatcaacaa agcgcagatt
    14581 tacgcctggc gcaagggtat caaaacgctc tattacattc gcctgcgtca gatggcgctg
    14641 gaaggcactg aaattgaagg ctgcgtctcc tgtgcacttt aaggaatatc tatgaaactc
    14701 tcacgtatca gcgccatcaa ctggaacaag atatctgacg ataaagatct ggaggtgtgg
    14761 aatcgcctga ccagcaattt ctggctacca gaaaaggtgc cactgtcgaa cgatatacct
    14821 gcatggcaga cattaagtgt cgtagaacaa caactgacaa tgcgcgtttt tactggcctg
    14881 acgctgctcg acacgctgca aaatgttatc ggcgcgcctt cgctgatgcc cgatgcgctc
    14941 acgcctcatg aagaggcagt attatcgaat atcagcttta tggaggcggt tcatgcccgc
    15001 tcttacagtt cgattttctc gacgctgtgc cagaccaaag atgtcgatgc cgcctacgcc
    15061 tggagtgaag aaaacgcgcc gttgcagcga aaagcacaga ttattcagca acattatcgc
    15121 ggtgatgatc cgctgaaaaa gaaaatcgcc agtgtgtttc ttgaatcttt tttgttctat
    15181 tccggtttct ggctgccgat gtatttttcc agccgcggaa agctaaccaa taccgcagac
    15241 ctgatccatc tgattatccg cgatgaagca gtccacggtt actacatcgg ctataaatat
    15301 cagaaaaacc tggaaaagat ctcccaggca cgacgtgaag aattgaagag tttcgccttc
    15361 gatttgttgc tggaactcta tgacaacgag ttgcaataca ccgatgagct gtacgccgaa
    15421 accccgtggg ctgacgatgt gaaagcgttt ctctgttaca acgccaataa ggcgttgatg
    15481 aatctgggct acgagccgct atttcccgca gaaatggcgg aagtgaatcc ggcaatcctc
    15541 gccgcgcttt cgccgaatgc cgatgaaaat cacgatttct tttccggttc aggctcttca
    15601 tatgtgatgg gaaaagcggt cgaaacagaa gatgaagact ggaatttctg agggggttat
    15661 tttcaaaaat atcactaccc gctgcaggga aataattccc gccaaatagc tttatatcac
    15721 gtaaataatt tgtggtgatc tacactgata ctctgttgca ttattcgcct gaaaccacaa
    15781 tattcaggcg tttttttgct atctttgaca aaaaatatca acttttctcg atttgctctc
    15841 agcccttata tcatgggaaa ttccggcgat ttgctcacat caatattcat gccacatttg
    15901 ccctcagggg ttgcctcaga ttctcagtat gttagggtag aaaaaagtga ctatttccat
    15961 tgggtaatgt atcgacataa acaaataaca ggaatctttc tattgcatgg caattaaatt
    16021 agaaattaaa aatctttata aaatatttgg tgagcatcca cagcgagctt tcaaatatat
    16081 cgaacaagga ctttcaaaag aacaaattct ggaaaaaact gggctatcgc ttggcgtaaa
    16141 agacgccagt ctggccattg aagaaggcga gatatttgtc atcatgggat tatccggttc
    16201 gggtaaatcc acaatggtac gccttctcaa tcgcctgatt gaacccaccc gcgggcaagt
    16261 gctgattgat ggtgtggata ttgccaaaat atccgacgcc gaactccgtg aggtgcgcag
    16321 aaaaaagatt gcgatggtct tccagtcctt tgccttaatg ccgcatatga ccgtgctgga
    16381 caatactgcg ttcggtatgg aactggccgg aattaatgcc gaagaacgcc gggaaaaggc
    16441 ccttgatgca ctgcgtcagg tcgggctgga aaattatgcc cacagctacc cggatgaact
    16501 ctctggcggg atgcgtcaac gagtgggatt agcccgcgcg ttagcgatta atccggatat
    16561 attattaatg gacgaagcct tctcggcgct cgatccatta attcgcaccg agatgcagga
    16621 tgagctggta aaattacagg cgaaacatca gcgcaccatt gtctttattt cccacgatct
    16681 cgatgaagcc atgcgtattg gcgaccgaat tgccattatg caaaatggtg aagtagtaca
    16741 ggtcggcaca ccggatgaaa ttctcaataa tccggcgaat gattatgtcc gtaccttctt
    16801 ccgtggcgtc gatattagcc aggtgttcag tgcgaaagat attgcccgtc gtacaccaaa
    16861 cggcttaatt cgtaaaaccc ccggcttcgg cccgcgttcg gcactgaaat tactgcagga
    16921 tgaagatcgc gaatatggct acgtcatcga acgcggtaat aagtttgtcg gcgctgtgtc
    16981 tatcgattcg cttaaagccg cgttaacgca gcagcaaggt cttgatgcgg cgctgattga
    17041 tgcgccgtta gcggtcgatg cacaaacgcc tcttagcgag ttgctctctc atgtcggaca
    17101 ggccccctgt gcggtgcccg tggtcgatga ggaccaacag tatgtcggca tcatttcgaa
    17161 aggaatgctg ctgcgcgctt tagatcgtga gggggtaaat aatggctgat caaaataatc
    17221 cgtgggatac cacgccagcg gcggacagtg ccgcacagtc cgcagacgcc tggggtacac
    17281 cggctgctgc accgactgac ggcggtggtg ctgactggct gaccagtacg cctgcgccaa
    17341 acgtcgagca ttttaatatt ctcgatccgt tccataaaac gctgatcccg ctcgacagtt
    17401 gggtcactga agggatcgac tgggtcgtta cccattttcg tcccgtcttt cagggcgtgc
    17461 gcgttccggt tgattacatc ctcaacggtt tccagcaatt gctgctgggt atgcccgctc
    17521 cggtggcgat tatcgttttc gctctcatcg cctggcaaat ttccggtgtc ggaatgggcg
    17581 tggcgacact ggtttcgctg attgccatcg gcgcaatcgg tgcctggtca caggcaatgg
    17641 ttactctggc gctggtgcta accgccctgc tgttctgtat cgtcatcggt ttgccgttgg
    17701 ggatctggct ggcgagaagt ccgcgagcgg cgaaaattat tcgtccactg cttgatgcca
    17761 tgcagaccac gcccgcgttt gtttatctgg taccaatcgt catgctgttt ggtatcggta
    17821 acgtgccggg cgtggtggtg acgatcatct ttgctctgcc gccgattatc cgtctgacga
    17881 ttctgggaat taaccaggtt ccggcggatc tgattgaagc ctcgcgctca ttcggtgcca
    17941 gcccgcgcca gatgctgttc aaagttcagt taccgctggc gatgccgacc attatggcgg
    18001 gcgttaacca gacgctgatg ctggcccttt ctatggtggt catcgcctcg atgattgccg
    18061 tcggcgggct gggtcagatg gtgcttcgcg gtatcggtcg tctggatatg gggcttgcca
    18121 ccgttggcgg cgtcgggatt gtgatcctcg ccattatcct cgaccgcctg acgcaggccg
    18181 ttgggcgcga ctcacgcagt cgcggcaacc gtcgctggta caccactggc cctgtcggtc
    18241 tgctgacccg cccattcatt aagtaactct gcacttgccc ggtgacgccg ggcattatca
    18301 ccctgccaaa aaaaggaata acaatgcgac atagcgtact ttttgcgaca gcgtttgcca
    18361 cgcttatctc tacacaaact tttgctgccg acctgccggg caaaggcatt actgttaatc
    18421 cggttcagag caccatcacc gaagaaactt tccagacgct gctggtcagc cgcgcgctgg
    18481 agaaattagg ttataccgtc aataaaccca gcgaagtgga ttacaacgtt ggctacacct
    18541 cgcttgcttc cggcgatgca accttcaccg cagtgaactg gacgccactg catgacaaca
    18601 tgtacgaagc tgccggtggc gataagaaat tttatcgtga aggagtattt gttaacggcg
    18661 cggcacaggg ttacctgatc gataagaaaa ctgccgacca gtacaaaatt accaacatcg
    18721 cgcaacttaa agatccgaag atcgccaaac tgttcgatac caacggcgac ggtaaagcgg
    18781 atttaaccgg ctgtaaccca ggctggggct gcgaaggtgc gatcaaccac cagcttgccg
    18841 cgtatggact gaccaatacc gtgacgcata atcaggggaa ctacgcagcg atgatggctg
    18901 acaccatcag tcgttacaaa gagggtaagc cggtgtttta ctacacctgg acgccgtact
    18961 gggtgagtaa cgaactgaaa ccggggaaag atgtggtctg gttgcaggtg ccgttctccg
    19021 cactgccggg cgataaaaat gccgatacca aactgccgaa tggcgcgaat tacggcttcc
    19081 cggtcagtac catgcatatc gttgccaaca aagcctgggc cgagaaaaac ccggcagcag
    19141 cgaaactgtt tgccattatg cagttaccag tggctgatat taacgcccag aacgccatta
    19201 tgcatgacgg caaagcctca gaaggcgata ttcagggcca cgttgatggc tggatcaaag
    19261 cccaccagca gcagttcgat ggctgggtga atgaggcgct ggcagcgcag aagtaatttt
    19321 tattcgggcg gataaggcgt ttttgcctca tccgccgctc tgtacacaat gcctgatgcg
    19381 acgctggtgc gtcttatcat acctacaaac cacaccgcac atttcaacaa tctatctttc
    19441 atcccatatt catcaacatc cgctattatt gatttccagc ttaatcatca cctgatgaac
    19501 aaaaataatg actaaaccta atcatgagct tagcccggcg ctgatcgtgc tgatgtctat
    19561 cgccaccggc ctggcggtag ccagtaacta ttacgcccag ccattgctcg acaccatcgc
    19621 gcgtaacttt tccctttccg ccagttcggc aggctttatt gttaccgccg cgcagttggg
    19681 ctatgccgca ggtctgctgt ttcttgttcc cctcggtgat atgtttgaac gccgccgcct
    19741 gattgtctcg atgaccttac tggcggcatg cggcatgtta atcactgcca gcagtcagtc
    19801 gctggcgatg atgatcctcg gtacggcatt aaccggttta ttctcggtcg tggcacaaat
    19861 tctggttccg ctggcagcga cgctggcttc accggacaaa cgcggcaaag tggtcggcac
    19921 cattatgagc ggtctgctgt tggggatctt gctggcacga accgttgccg ggttgctggc
    19981 aaacctcggc ggctggcgca cagtcttttg ggttgcctcg gtgttaatgg cactgatgac
    20041 gctggcatta tggcgtggtc tgccacaaat gaaatcagaa acccacctca actacccaca
    20101 gttgcttggt tccgttttca gcatgtttat cagcgataag atcctgcgca cccgtgcgtt
    20161 gctgggctgc ctgacctttg ccaacttcag cattctctgg acctcaatgg cctttttgct
    20221 tgccgctcca ccttttaact acagcgatgg cgtcattggt ctgtttggac ttgcgggagc
    20281 tgccggagcg ttgggtgctc gtccggcggg cggttttgcc gataagggca aatcgcacca
    20341 caccacaact ttcggtctgc tgctgctgtt actttcatgg ctggcaatct ggtttggtca
    20401 cacttccgta ctggcgctga ttatcggcat cctggtgctg gacctcaccg tgcagggcgt
    20461 gcatatcact aaccagacgg taatttatcg aatacaccct gatgcgcgta atcgcctgac
    20521 cgcaggttac atgaccagct actttattgg cggtgccgcc ggttcgctaa tttcagcctc
    20581 agcctggcaa catggcggtt gggctggcgt ttgtctggct ggcgcgacga ttgccctggt
    20641 taacttactg gtctggtggc gaggttttca tcgtcaggaa gccgcaaatt aagcaatcag
    20701 caaccgtttt cgtggcttta cacttataag ggtgttaaga agcccatcag tctgataagg
    20761 ttaagatatt cattcagtct atttataata ttaacaatcg ttaagcgtac actctatgga
    20821 aagccctact ccacagcctg ctcctggttc ggcgaccttc atggaaggat gcaaagacag
    20881 tttaccgatt gttattagtt atattccggt ggcctttgcg ttcggtctga atgcgacccg
    20941 tctgggattc tctcctctcg aaagcgtttt tttctcctgc atcatttatg caggcgcgag
    21001 ccagttcgtc attaccgcga tgctggcagc cgggagtagt ttgtgggttg ctgcactgac
    21061 cgtcatggca atggatgttc gccatgtttt gtatggcccg tcactgcgta gccgtattat
    21121 tcagcgtctg caaaaatcga aaaccgccct gtgggcattt ggcctgacgg atgaggtttt
    21181 tgccgccgca accgcaaaac tggtacgcaa taatcgccgc tggagcgaga actggatgat
    21241 cggcattgcc ttcagttcat ggtcatcgtg ggtatttggt acggtaatag gggcattctc
    21301 cggcagcggc ttgctgcaag gatatcccgc cgttgaagca gcattaggtt ttatgcttcc
    21361 ggcactcttt atgagtttcc tgctcgcctc tttccagcgc aaacaatctc tttgcgttac
    21421 cgcagcgtta gttggtgccc ttgcaggcgt aacgctattt tctattcccg tcgccattct
    21481 ggcaggcatt gtctgtggct gcctcactgc gttaatccag gcattctggc aaggagcgcc
    21541 cgatgagcta tgaggttctg ctgcttgggt tactggttgg cacggcgaat tattgcttcc
    21601 gctatttgcc gctgcgcctg cgtgtgggta atgcccgccc aaccaaacgt ggcgcgatag
    21661 gcatattgct cgacaccatt ggcatcgcct cgatatgcgc tctgctggtt gtctctaccg
    21721 caccagaagt gatgcacgat acacgccgtt tcgtgcccac gctggtcggc ttcgcggtac
    21781 tgggtgccag tttctataaa acacgcagca ttatcatccc aacactgctt agtgcgctgg
    21841 cctacgggct cgcctggaaa gtgatggcga ttatataacc cacaagaatc atttttctaa
    21901 aacaatacat ttactttatt tgtcactgtc gttactatat cggctgaaat taatgaggtc
    21961 atacccaaat ggatagttcg tttacgccca ttgaacaaat gctaaatttt cgcgccagcc
    22021 gccacgaaga ttttccttat caggagatcc ttctgactcg tctttgcatg cacatgcaaa
    22081 gcaagctgct ggagaaccgc aataaaatgc tgaaggctca ggggattaac gagacgttgt
    22141 ttatggcgtt gattacgctg gagtctcagg aaaaccacag tattcagcct tctgaattga
    22201 gttgtgctct tggatcatcc cgtaccaacg cgacgcgtat tgccgatgaa ctggaaaaac
    22261 gcggttggat cgaacgtcgt gaaagcgata acgatcgccg ctgcctgcat ctgcaattaa
    22321 cggaaaaagg tcacgagttt ttgcgcgagg ttttaccacc gcagcataac tgcctgcatc
    22381 aactctggtc cgcgctcagc acaacagaaa aagatcagct cgagcaaatc acccgcaaat
    22441 tgctctcccg tctcgaccag atggaacaag acggtgtggt tctcgaagcg atgagctaac
    22501 gcgtcatctc gctcaaaaat ccagatttat aaaagaaaaa atgactggcc agcatcgcaa
    22561 catgctggcc tttttggcaa gcaggtcggc tcagccgatg agttaagaag atcgtggaga
    22621 acaatatgag cgcaaatgcg gagactcaaa ccccgcagca accggtaaag aagagcggca
    22681 aacgtaagcg tctgctcctc cttctcacct tgctctttat aattattgcc gtagcgatag
    22741 ggatttattg gtttttggta ctgcgtcatt tcgaagaaac tgatgacgca tacgtggcag
    22801 ggaatcagat tcaaattatg tctcaggtgt ctggcagcgt gacgaaagtc tgggccgata
    22861 acaccgattt tgtaaaagaa ggcgatgtgc tggtcactct cgacccgaca gatgctcgcc
    22921 aggcgtttga aaaagccaaa actgcactgg cttccagcgt tcgccaaacc caccagctga
    22981 tgattaacag caaacagttg caggcgaata ttgaggtgca aaaaatcgcc ctcgcgaaag
    23041 cacaaagcga ctacaaccgc cgtgtgccgc tgggcaatgc caacctgatt ggtcgcgaag
    23101 agttgcaaca cgcccgcgac gccgtcacca gtgcccaggc gcaactggac gtcgctattc
    23161 aacaatacaa tgccaatcag gcgatgattc tggggactaa actggaagat cagcctgccg
    23221 tacaacaggc tgccaccgaa gtacgtaacg cctggctggc gctggagcgt acccgtattg
    23281 tcactccgat gaccggttat gtctcccgcc gcgcggtaca gcctggggcg caaattagcc
    23341 caacgacgcc gctgatggcg gtcgttccag ccaccaatat gtgggtggat gccaacttta
    23401 aagagacgca gattgccaat atgcgtatcg gtcaaccggt caccatcacc accgatattt
    23461 acggcgatga tgtgaaatac accggtaaag tggttggtct ggatatgggc acaggtagcg
    23521 cgttctcact gcttccggcg caaaacgcca ccggtaactg gatcaaagtc gttcagcgtc
    23581 tgcctgttcg tattgaactg gaccagaaac agctggagca atacccgctg cgtatcggtt
    23641 tatccacgct ggtgagcgtc aataccacta accgtgacgg tcaggtactg gcaaataaag
    23701 tacgttccac accggtagcg gtaagcaccg cgcgtgaaat cagcctggca cctgtcaata
    23761 aactgatcga cgatatcgta aaagctaacg ctggctaatc cagaggtgcg tgtgatgcaa
    23821 cagcaaaaac cgctggaagg cgcgcaactg gtcattatga cgattgcgct gtcactggcg
    23881 acattcatgc aggtgctgga ctccaccatt gctaacgtgg cgatccccac tatcgccggg
    23941 aatctgggct catcgctcag ccagggaacg tgggtaatca cttctttcgg ggtggcgaat
    24001 gccatctcga tcccgcttac cggctggctg gcaaagcgcg tcggggaagt gaaactgttc
    24061 ctctggtcca ccatcgcctt tgctattgcg tcgtgggcgt gtggtgtctc cagcagcctg
    24121 aatatgctga tcttcttccg cgtgattcag gggattgtcg ccgggccgtt aattccgctt
    24181 tcgcaaagtc tattgctgaa taactacccg ccagccaaac gctcgatcgc gctggcgttg
    24241 tggtcgatga cggtgattgt cgcgccaatt tgcggcccga tcctcggcgg gtatatcagc
    24301 gataattacc actggggctg gatattcttc atcaacgtac cgattggcgt ggcggtggtg
    24361 ttgatgacac tgcaaaccct gcgcggacgc gaaacccgca ccgaacggcg gcggattgat
    24421 gccgtggggc tggcactgct ggttattggt atcggtagcc tgcagattat gctcgaccgc
    24481 ggtaaagagc tggactggtt ctcatcacag gaaattatca tccttaccgt ggtggcagtg
    24541 gtggctatct gcttcctgat tgtctgggag ctgaccgacg ataacccgat agtcgatctg
    24601 tcgttgttta agtcgcgcaa cttcaccatc ggctgtttgt gtatcagcct cgcgtatatg
    24661 ctctacttcg gcgctattgt tctgctgccg cagttgttgc aggaggtcta cggttacacg
    24721 gcaacgtggg caggtctggc ctctgcgccg gtaggaatta ttccggtgat cctgtcgccg
    24781 attatcggcc gcttcgcgca taaactggat atgcggcggc tggtaacctt cagctttatt
    24841 atgtatgccg tctgcttcta ctggcgtgcc tatacctttg aaccaggtat ggattttggc
    24901 gcgtcggcct ggccgcagtt tatccagggg tttgcggtgg cctgcttctt tatgccgctg
    24961 accaccatta cgctgtctgg tttgccaccg gaacgactgg cggcggcatc gagcctctct
    25021 aactttacgc gaacgctggc ggggtctatc ggcacatcca tcaccaccac catgtggacc
    25081 aaccgcgagt cgatgcacca tgcgcagttg actgagtcgg taaatccgtt caacccgaat
    25141 gctcaggcga tgtacagtca actggaaggg cttggtatga cgcaacagca ggcatcaggc
    25201 tggattgccc agcagataac caatcagggg ctgattattt ccgctaatga gatcttctgg
    25261 atgtcagccg ggatattcct cgtcttgctg gggctggtgt ggtttgctaa accgccattt
    25321 ggcgcaggtg gcggcggagg tggtgcgcac taagtacaac taagccagtt catttgaact
    25381 ggcttttttc aattaattgt gaagatagtt tactgactaa atgtgcagtt cctgcaactt
    25441 ctctttcggc agcgccagtt cttcgttgct gttgatgcgc acgtcacgtt ccagaatgtt
    25501 acgcgcaata tcctgcgctt cctgcaacga gtgcatctgg taagtgccac actggtagac
    25561 gttcagttcc gggatctgat tctgatcctg cactttcagc acgtcttcca ttgccgcttt
    25621 ccaggcatca gcgacacgct gctcatctgg cgtaccaatc agactcatat aaaaaccggt
    25681 gcggcagccc attggcgaga tatcgataat ctctacacca ttaccgttaa gatggttacg
    25741 cataaaacca gcaaacaggt gctccagggt atggatccct ctttctggca tcacttcttt
    25801 gttcggcacg cagaagcgca gatcgaacac ggtgattgcg tcgccatgcg gggtgttcat
    25861 tgttttcgcc acccgaactg caggcgcttc catccgggta tgatcgactg tgaagctatc
    25921 taacaacggc atttagccac ctccggtaat ttttttaaaa attttctgaa ctctttcttc
    25981 ccaggcgagt ctgagtatat gaaagacgcg catttgttat catcatccct gaattcagag
    26041 atgaaatttt ggccactcac gagtggcctt tttcttttct gtcaggcgtg tttttccagc
    26101 cacaccgcaa acggttcggt atcagcggct tccatttcct gctgacggcg ttcagacgcc
    26161 tcgcgctcgg ctacaaaatc ctcttcgcgc agaatttcca gcggctcttc acgcagcaga
    26221 ttacggtagg cttctgcaaa cgctttgcct gttccgccaa taccagtatc aatcatagac
    26281 cttaagatac gggcagagaa agtcagatcg ggattatcga agcaggcgac cagttcatca
    26341 cacactttct gatacgcttc gccgccgtta atgctatcca gcgtttgcgc gacgcgtttc
    26401 agatcgcgga acagatcttt acccacctgc ggtaacggga actgcgcagt ttcgcagccg
    26461 atacccagcg tcaggcccgg tttgcgacct tcgaggatca cccggttcca gttaacgcgt
    26521 gtacaggcaa gttcgctact gctcatttcc ggtgcgtcag ccagcgcaca ccagaccata
    26581 aacaggtcga ggaatcgcac ctgttgttca tctacaccaa tcggcgagaa cgggttgata
    26641 tccagcgagc ggacttcaat atattcaatg ccgccacgta acagcgcatc agaaggcgac
    26701 tcaccactgc gggtaacgcg ttttggacga atcggcgcgt acagttcgtt ttcaatctgc
    26761 agcacattgc tgttgatttg cagccgctta ccgtctttct caataccaat cttcgcgtac
    26821 tcttccgatg gcgttttgat tgcctgttta aggcccgcca cgtactcgta gagatcattg
    26881 aaggtgatac caagattgct ttgcgattta ttggtatagc caagatcgct caaacgaaga
    26941 gaggtcgcat acggcaggta atacataccg cactcggttt tctcaaacgg cagcgacgtt
    27001 ggttttcctt gcaagaaaga agaacaaatc gctggagacg caccaaacag ataaggaatg
    27061 acccaaccga agcgatagta gttacggata acacggaaat aacccgcaga tattttctct
    27121 ttggcatcag cgcccgagat atcaccgcac ttcgcttgcc agaatgccat tggcaaagag
    27181 aaattatagt gcacgccgga aatggtttgc atcagcgcgc cgtagcgatt tttcagccct
    27241 tcacggtaca gcgttttaaa gcgaccggtg ttagaagtgc cgtactgtgc cagttcgatg
    27301 tcctgacctt ctgcgatgta gcatggcata cttaacggcc acatccgctc atcgcccata
    27361 ttgcgcgccg tataacgatg cagatcgcgc ataaaggtca acatatgttc aatatcacca
    27421 tccactggtg taatgaattc cagcaatgct tccgcaaaat cggtagtaat ccatttgtgc
    27481 gtcagtgcgg aacctaatgc ttcaggatga cctgttgttg ccagtgttcc atcagcatta
    27541 acacgcaaag tttcgcgctc cagcccacgc tgtatcccct ttaacgcctg aggatgtttt
    27601 tccagccagg ccagcgcctg tgatacgtcc gggatcaaat tgacctcccg cctgtcaaaa
    27661 tcgttttaat tagcataact gtaatggtga ccatatgtgc aggcctacaa ttagtgccac
    27721 cacatcatgc cctgaacggt cgctgctgca actgcaacat agcgtaacgc tttaccaagg
    27781 cacaaaaaaa agattaccgg cccccaggag atgcgcatcc atcccgctaa cagacacagt
    27841 aaatcgccaa ccacgggcat ccagcttaat aatagtgtga ctgcaccata gcgtttcagc
    27901 cagccggtag ctttctcttg ccagcgcgat gttttacgca atggaaagaa acgcccaagg
    27961 ataacgttag ttaaccctcc aaggctatta cccattgttg ctgttaaaac taaaacccag
    28021 ggatgactga tcccggaaag caacattgcc accagcacga cttcggagtt gccgggtaat
    28081 agtgtagcgc tgagaaaact actggcaaac aacgagaaaa gcgataacgc ttcactcaca
    28141 gcaagcgaac atccacggcg tccatgcctg ctgcacgggc cgcctgaata ccgaaatcgg
    28201 catcttcaaa gaccacacac tgcgtcggtt gcacgcccat acgctgcgcg cacaacaaaa
    28261 atgtgtctgg cgcgggttta tggtgtttga cgtgatcggc agcgacgacg gcgtcaaaat
    28321 aacggcgtaa tcccaggtgt gccagcaatg cctcagcgat ggcgctttca ctccccgttc
    28381 ctacagccat tgggcgacga ccatgccagc ttttcaccac ttcaacaaga ggaagcggct
    28441 cgacactatc cagcagcata cttcttactg cttctgtttt ttcacgcgct aacgcatgcg
    28501 ggtcgagatc ggcctgattc agctcaataa ttgcctgagc aatacgccag gtgggcgagc
    28561 cattaagcgc aatcatcgcc tgaacatcgt actgaagacc gtagtgccct aatacttcgc
    28621 gccacgcttt acggtgcgta ggctccgtat ccaggattgt gccatccata tcaaaaatta
    28681 aacctgcata acgctcgtac atggtcttct cgcaagtcga aaaatatgac gctactttag
    28741 cgtaattgct tgattttgtc gctgatggcg aaggagaaga aaattcagga aatggataaa
    28801 gtagttattt agaaatgaga tatttttgaa ggaaattttt tggag
//
LOCUS       DAPHCV010000063        28545 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.063, whole genome shotgun sequence.
ACCESSION   DAPHCV010000063 DAPHCV010000000
VERSION     DAPHCV010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.063"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(344..2197)
                     /gene="asmA"
                     /locus_tag="RH033_002691"
     CDS             complement(344..2197)
                     /gene="asmA"
                     /locus_tag="RH033_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HDV2991857.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEQPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(2219..2800)
                     /gene="dcd"
                     /locus_tag="RH033_002692"
     CDS             complement(2219..2800)
                     /gene="dcd"
                     /locus_tag="RH033_002692"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HDV2991858.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDESEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(2892..3533)
                     /gene="udk"
                     /locus_tag="RH033_002693"
     CDS             complement(2892..3533)
                     /gene="udk"
                     /locus_tag="RH033_002693"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDV2991859.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            3851..7168
                     /locus_tag="RH033_002694"
     CDS             3851..7168
                     /locus_tag="RH033_002694"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDV2991860.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQSNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMLAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSAMLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAENVQKLRLGGCRIIFSQVGRDLQIFNSLKANMADYLLLDD
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGEVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(7269..8131)
                     /gene="alkA"
                     /locus_tag="RH033_002695"
                     /pseudo
     CDS             complement(7269..8131)
                     /gene="alkA"
                     /locus_tag="RH033_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
     gene            8265..9617
                     /gene="yegD"
                     /locus_tag="RH033_002696"
     CDS             8265..9617
                     /gene="yegD"
                     /locus_tag="RH033_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDV2991861.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANTQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRNAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(9630..11570)
                     /gene="yegI"
                     /locus_tag="RH033_002697"
     CDS             complement(9630..11570)
                     /gene="yegI"
                     /locus_tag="RH033_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HDV2991862.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDIEEFADSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLPSFVGFERTANHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDIAHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCTVSAMHVYPAHLTDCPWCTLDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPSGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRTMLEKMKDEMLGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDAASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRTNEAVTPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(11567..12328)
                     /locus_tag="RH033_002698"
     CDS             complement(11567..12328)
                     /locus_tag="RH033_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HDV2991863.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAKLAVNEAMAFMVQKVQHGEFGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     SDEDAVSRLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLAAATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(12325..12984)
                     /locus_tag="RH033_002699"
     CDS             complement(12325..12984)
                     /locus_tag="RH033_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDV2991864.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLSLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(13205..13264)
                     /gene="ibsA"
                     /locus_tag="RH033_002700"
     CDS             complement(13205..13264)
                     /gene="ibsA"
                     /locus_tag="RH033_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HDV2991865.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(13537..13593)
                     /locus_tag="RH033_002701"
     CDS             complement(13537..13593)
                     /locus_tag="RH033_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDV2991866.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            complement(13865..13921)
                     /locus_tag="RH033_002702"
     CDS             complement(13865..13921)
                     /locus_tag="RH033_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDV2991867.1"
                     /translation="MMKILIIVVLLVISYPAY"
     gene            14200..15447
                     /gene="mdtA"
                     /locus_tag="RH033_002703"
     CDS             14200..15447
                     /gene="mdtA"
                     /locus_tag="RH033_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HDV2991868.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNEPQSAAPGATKQAQ
                     HPPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATAPEEKATSREYAKKGARS"
     gene            15447..18569
                     /gene="mdtB"
                     /locus_tag="RH033_002704"
     CDS             15447..18569
                     /gene="mdtB"
                     /locus_tag="RH033_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HDV2991869.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASERMFDRIIAAYGRGLTKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGIDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            18570..21647
                     /gene="mdtC"
                     /locus_tag="RH033_002705"
     CDS             18570..21647
                     /gene="mdtC"
                     /locus_tag="RH033_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HDV2991870.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIVISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            21648..23063
                     /locus_tag="RH033_002706"
     CDS             21648..23063
                     /locus_tag="RH033_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2991871.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNHRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            23060..24463
                     /gene="baeS"
                     /locus_tag="RH033_002707"
     CDS             23060..24463
                     /gene="baeS"
                     /locus_tag="RH033_002707"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HDV2991872.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            24460..25182
                     /gene="baeR"
                     /locus_tag="RH033_002708"
     CDS             24460..25182
                     /gene="baeR"
                     /locus_tag="RH033_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HDV2991873.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            25373..25705
                     /locus_tag="RH033_002709"
     CDS             25373..25705
                     /locus_tag="RH033_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HDV2991874.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            25949..26200
                     /locus_tag="RH033_002710"
     CDS             25949..26200
                     /locus_tag="RH033_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003915633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HDV2991875.1"
                     /translation="MARTMTVDLGDELREFIESLIESGDYRTQSEVIRESLRLLREKQ
                     AESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            26202..26498
                     /locus_tag="RH033_002711"
     CDS             26202..26498
                     /locus_tag="RH033_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDV2991876.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            26601..27962
                     /gene="yegQ"
                     /locus_tag="RH033_002712"
     CDS             26601..27962
                     /gene="yegQ"
                     /locus_tag="RH033_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HDV2991877.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGESTRNP
                     HGK"
     gene            complement(28292..>28545)
                     /locus_tag="RH033_002713"
     CDS             complement(28292..>28545)
                     /locus_tag="RH033_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2991878.1"
                     /translation="DETSIGKFGLAYKSNIQRKLDNQYYTEAEASLARGRISGAENIV
                     KNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFKCGE"
BASE COUNT         6548 a         7674 c         7547 g         6776 t
ORIGIN      
        1 tcgccgaatc gtttcctgta ggccggggca gcccacatgg atgtgggctg agggcgcgtt
       61 ttacagggat gttacctcgc gcccgtaccc gatagccgaa aggaataaga tgagggaacc
      121 gcgccagcgg cgatttaccg ccgggagccc gggttgccag ggtggtggcg attgagccac
      181 cctggcacgt tcaccggctt gagcgttcca tagtagcgag gaacatcaag tgaacggaac
      241 agccttcgat cttacatatt cagaatataa tggtgccgcc gcgaacgcct gatgcgacgc
      301 tttacgcgtc ttatcaggcc tacaggacag gtcacctggt aacctacatc ttctccagca
      361 acttcttcac atctttgcca ttgcgggaat ctttattccg ctcagcccag tcattcaggc
      421 gacgtttcgc ttcgtcctgt agatgtttgc gcagtaactg atccacttgc agactgtaat
      481 tgagttgctg ccagttgcca taaacccgca gcggtactgg cgtttctttg aggaaatcaa
      541 tcagtttgct ttccccgttc cagccaccca cgacccgaat atcaaactgg gtgtcgcagg
      601 tttgatctgc cagattcaac ataccttccc ctgtaagcgc cagcacaggc gattgacctt
      661 gcatgtcgtt taacgtcacg acgccatcct tcaatgtcaa atcagtggta aagcggtcaa
      721 gccgcgtcac gttatcgaaa ttttcagcgg ccttcacatc accaccatta cgttctaccg
      781 cttgctgaat catctgctgg aagttcatcc cttccatgcg cgtgtcggtc atttcgacat
      841 gtgcctgtcc ttgccagttg tggcggaatg cgtcggcatc tatgtcagca ccggagaaat
      901 caccagccag tgacatcttt ccggtcaacg aaatcggata gttaaacgcc ttcagaatgg
      961 tgccaatctc aacgttttcc agccgtggct ggaagtttat ccgcggattt attgatgtcg
     1021 cgtccagcgt gcccggcagt gaaacctgtc caccgttaag cttgccctgc agttgagtaa
     1081 tttccagcaa acccgacttg ttggtcattt gcgtggcaac atctgtaaaa ttcattccgc
     1141 gccagcgcac gttactggcc tgcaacaaaa tatcagccgt aaagccttgc agtccctgat
     1201 aggccggttc atcaatacgc gaagaaatga ccgggcgcgg caacgttgat tggctctgcc
     1261 cctgctgcgc ggcaccgttt tcaccattcg cggtttcatt aagcgggatg aggttgtcaa
     1321 gattcagttg cgggaattgc agtctcagct gccattccgg ttgctctgtc agcgtgacct
     1381 gtgcttgccc gctcagcgta ctgtcattgg cggtcaagct gatttggtta aatgaaaggc
     1441 gtttatgtga ctcctgccac tgggcctgga aactcccctg cccctgaata ccttgttttg
     1501 gtaaatcggc accctgcaac tgccagttaa tttgttcaat agccgccgtt aaatcatgcg
     1561 gataatcaga agcatccacc gtaccgttaa gggatattgt gagatcgcgc tgatcgcgat
     1621 taacccgccc ggagaactca aatgagccac gatgttgggg atcttgttcc atttgcaggc
     1681 ggatattgcg gattgtcacc tgctcgtcat cttcatgctg gaacaccagc acgctgtccg
     1741 ccaccttaag actggatata tcaaacgacc atccgcgatc gtctgacaga tccggcaagg
     1801 tattgtcgcg cggtgcaacc ggagcgtctt cactgcgcac cgcttccgtc tgcggcgtca
     1861 gttggatcac tgcccctttt agcatcacct gcttaacgct cagttgatga ctcagtagtg
     1921 gtaaaagcgc cacgtccagg cgcatgttgt cggcgcgaac cagtggctgg ctcgcgccct
     1981 gggcggtgag agacattcgc ccggagagga tactaagttg cggccagacg tgccaacgca
     2041 gtggcccgtc gagctgcaat tgataaccgc tacgtgcagc aacttgcttg accatatagt
     2101 cgcggaaatc attcggattc accagcaaca ctaacgcaga taacccggcc accagcacga
     2161 ccaggagtat catcagcgtc gtcagaaatc gtctcatggt atcctcaatg ggcctgaatt
     2221 agtctttatc gattcggctg gctaccgcgc cctgctggtt gcgatatttc gcatcttcac
     2281 ggcggttgta aggtcgcgcc gccgggccgg aaagcggctc aaagctcagc gcaccaatta
     2341 acatgcccgg acgcagcgcc agcggcagct taccggagtt gtagaactcc agcacaatgc
     2401 agccagacca gcccggatcg atgcggtgcg cggtgacgtg caccatcagc cccagacgcg
     2461 ccagtgagga acgcccgtcc agccagccca ccagatcggc tggcagcgtc accgactcca
     2521 gcgtcaccgc cagcgccaac tctcctgggt gaagatagaa cgcctcgctt tcatcgagaa
     2581 cgatctcatc gctcatcacg cggtcaagcg cggcgctcac ttcatctttg ggaccgctca
     2641 gatcgataaa cgctgccgtg tgaccacgga aggtacgaaa tttattgccc aggcgcacat
     2701 ccaccgtcgc gccgttaata cgctccactg gcggacgtgg gttgatcgac aaacggcctt
     2761 catcaagcca ggcttcaata tctcggtcac acagacgcat ggcattttct cctttcgcgc
     2821 atcactccct taacgccaat tacgtcaagg gcataccagg ttatcactga acggtacaca
     2881 atttatcaag cttattcaaa gaactgactt attttcgctt tcaatatatc gatcgcgatg
     2941 cggtttttcc cgccgcgcgg cacgataatg tccgcatatt gtttagaagg ctcaatgaat
     3001 tgcaggaaca tcggacgcac ggttttttga tattgcgcca tcactgaatc cattgaacgc
     3061 ccacgctcgt taacgtcacg cttgatgcgg cgcatcaggc agatatccag cggggtatca
     3121 acgaaaatgg agaagttaag ttcgtcacgc aaacgcgcat ccgtcagcaa caaaatgcct
     3181 tcgagaatga tgactttctt cggctcaacc gtcaccgttt ctttcatacg cgtatgttca
     3241 acatagctgt aaaccggcag gtcaattgcc gagccgcgtt tcaacgcttg taaatgctca
     3301 agcagcaggc tgtgatccat cgcgctggga tggtcgtagt tggtcttaac gcgttcttcc
     3361 atcgacagat ggctttgatc tttgtaatag cagtcttcgg gaattacgcc gatgtgttca
     3421 tcaccgactt gctcacgtaa ttcacgataa agggtactgg caataagact cttgccggaa
     3481 gccgatgcgc cagcgatacc gataatgacg cactgatgag actgatcagt catatattta
     3541 gcgacctgat taacctggat gttaggaagg gggcgacgaa gcgccaaacg cggcaattat
     3601 agggatttca tccgcctgat accagtcgaa tagcgttgcc gcgcgctcag agttaattgt
     3661 tgacgaagaa ttcccggtgg caaattacgt tgatcagttt tatgtaaggt aaaaaatgtt
     3721 atacgcggtt gcgcaaatta tccgccttta cgtcacttta tgagcaattc gcatataaaa
     3781 tgtaaaactt ttgtactagc ataaacacag aaacgaatac tggcgacctg gccttgcgga
     3841 taaagcggta atgagcaaac aatcacagca tgtattaatt gccctgcccc acccgctgct
     3901 tcacctggtc agtttaggtt tagtctcgtt tatctttacc cttttctcgc ttgagctttc
     3961 gcagtttggc acccaactcg ccccactgtg gttcccgacg tccatcatga tggtggcgtt
     4021 ttatcgccat gccgggcgca tgtggccggg aattgcgctg agctgttcgc tgggaaatat
     4081 cgccgcatcc atcctgcttt tttccaccag ctcgctgaac atgacctgga cgaccatcaa
     4141 tattgttgaa gctgtggtcg gggcagtgct gctacgtaaa ttgctgccgt ggtataaccc
     4201 cttgcaaaat ctggctgact ggctgcgtct ggcactcggc agcgccattg ttccacctct
     4261 gttggggggt gttctggttg tcctgctgac gcccggagac gatcctctca gggcattttt
     4321 gatatgggta ctgtcagaat ccatcggcgc tctggcactg gtgccgctgg gattgttatt
     4381 taaaccacac tatctgctgc gccatcgcaa cccacggttg ctttttgagt cgctgctcac
     4441 gttagccatc acactgacgt taagctggct ttcgatgctg tatctgccgt ggccttttac
     4501 tttcattatt gtgctgttga tgtggagcgc cgtgcgcctg ccacgaatgg aagccttttt
     4561 gatcttcctt accacggtga tgatggtgtc actgatgatg gccgcggatc cctccttgct
     4621 tgctacgccg cgtacgtacc tgatgagcca tatgccgtgg ctaccgtttt tgctgatcct
     4681 gctgcccgcc aacatcatga ccatggtgat gtatgccttt cgtgcggaac gcaaacacat
     4741 ttccgaaagc gaaacccgct ttcgaaacgc gatggaatat tccgctatcg gcatggcgtt
     4801 agtgggcacc gagggacaat ggctgcaatc caacaaagca ctctgccagt ttctcggtta
     4861 cagtcaggaa gagctgcgcg gactcacctt tcagcaactg acctggccgg aggatctcaa
     4921 taaagatctc caacaggttg aaaagctgat aagcggtgaa ataaacacct attcaatgga
     4981 aaaacgttac tacaaccgca atggcgatgt tgtctgggcg ttgcttgccg tctctctggt
     5041 gcgccacacg gatggcacgc cgctctattt tatcgctcag attgaagaca ttaacgagct
     5101 aaaacgcacc gaacaggtca atcagcaact gatggaacgc atcacgctgg ctaacgaagc
     5161 gggcgggatt ggtatctggg agtgggagct gaagccgaat atttttagct gggataagcg
     5221 gatgttcgag ctgtatgaaa ttcctccgca tatcaaaccg aactggcagg tgtggtacga
     5281 gtgcgtgctg ccggaagatc gccagcatgc cgaaaaagtg attcgtgatt cgttgcaatc
     5341 acgctcgccc tttaaactgg aatttcgcat taccgtaaaa gacggtattc gccatatccg
     5401 cgccctcgcc aaccgggtac tgaataaaga aggcgaagtc gaacgcctgc tcggcattaa
     5461 tatggatatg actgaggtta aacagcttaa cgaggcattg tttcaggaaa aagagcgcct
     5521 gcacattacg ctggactcca tcggcgaagc cgtggtctgt attgatatgg cgatgaaaat
     5581 tacctttatg aatccagtgg cggagaagat gagcggctgg acgcaggaag aagcgttagg
     5641 tgttccgctc ctgacggtgt tgcatattac ttttggcgac aacggaccat taatggagaa
     5701 catttacagt gccgacacct cacgttccgc gatcgaacaa gatgtggtgt tgcactgtcg
     5761 gagcggcggc agttacgacg tgcattacag tattacgccg ttaagtactc tggacggcag
     5821 caatattggt tcggttctgg tgattcagga cgttaccgaa tcacgcaaaa tgctgcgcca
     5881 gctgagctac agcgcctccc atgatgcact aacacatctc gccaatcgcg ccagttttga
     5941 gaagcaactg cgtatcctgc tgcaaacggt aaacagtaca catcagcgac atgccctggt
     6001 gtttatcgat cttgatcgct ttaaagcggt gaatgacagc gccgggcatg cggcgggcga
     6061 cgctttgctg cgcgaactgg cgtcgttgat gctgagtatg ctgcgctcca gtgacgtgct
     6121 ggcgcggctc ggcggtgatg aatttggtct gctgctgcca gactgcaatg ttgaaagcgc
     6181 tcgttttatc gctacacgta ttatcagtgc tgtgaatgac tatcacttta tatgggaagg
     6241 ccgggtacat cgggtaggtg ccagcgccgg gattaccttg atcgatgaca acaatcatca
     6301 ggcggcagaa gtgatgtcgc aggctgatat cgcctgttat gcctccaaaa atggtggccg
     6361 gggccgggtg acggtttacg aaccgcagca agctgccgca catagcgaac gggcggcgat
     6421 gtcgcttgat gaacagtggc ggatgattaa agagaatcag ttgatgatgc tcgcccacgg
     6481 tgtcgcttcg ccacggatcc cggaagcgcg taatttgtgg ctgatttcac ttaagctctg
     6541 gagttgcgaa ggcgagatta ttgatgaaca aacatttcgt cgtagcttca gcgatccggc
     6601 gcttagccat gctcttgacc gccgggtatt ccacgaattt ttccagcagg ccgcaaaagc
     6661 ggttgccagt aaaggcataa gcatcgccct ccccctttcc gttgccggtt tgagtagcgc
     6721 catgctggtg aatgacctgc ttgagcagct ggaaaatagc cctctaccac cacggttatt
     6781 acatctgatt attccggctg aagcgatttt agatcacgca gaaaacgtgc aaaaactgcg
     6841 gctgggggga tgtcggatca tattcagtca ggtgggccgc gatctgcaaa tcttcaactc
     6901 gctgaaagcg aatatggcag attacctgct actcgatgat gagttatgcg ccaacgtgca
     6961 gggtaatttg atggatgaga tgctgattac gattattcag gggcacgctc agcgactcgg
     7021 gatgaaaacc atcgccgggc cagtcgtttt acccttagtg atggatacgc tttctggcat
     7081 cggcgtcgat ctgatttatg gtgaggtgat tgccgatgcc caaccgctgg atttgctggt
     7141 gaatagcagt tatttcgcga ttaactgagg gagcgtttgc ccattgcctg atgcgacgct
     7201 aacgcgtctt atcaggccta caaatcgctc attccgcaag gcgctcgcac cgcttccggc
     7261 gaccaacgtc atgcttcgtc tggctgccag ccttccgtat accagatatg caacagcgca
     7321 taagaacgcc agggcttcca gcgttcggca taacggcgga ggcataacgg cggatttgtg
     7381 ccggtgtcat tcccggaaat cgttgtttaa tcagataatc atccggcagg aaaacatctt
     7441 tcgcctgcca gccacgcaaa gcgaaataat tcgccgtcca gcgcccgata cccggaaaag
     7501 tttgcagcgt tttcatcgca tgctccacat cgcccggaat tgtcattggt aagctgccct
     7561 ccagcgccgc atttgccaga tgaatcagtg cctctgcccg tttcaacggc atacctaacg
     7621 cttttaatgc ctgcgggtct gctgctgcca gccgctgagg cgtggggaag cagatatact
     7681 ccagaaaatc atccagccgt tcgccataaa gctgtgccac tttggcggtc aattttgccg
     7741 ccatcgccac gctcaccagt tggcctaaaa tcgcccgcac gccctgctca aaagcatcaa
     7801 cacagccggg taaacgcaat ccgggccgcg ccgcgcctaa cctgcccaac gcaccgttaa
     7861 caatttgtgg gttacattgc agatcaaaca ggcggctcat tttcgccaga cactctgcgg
     7921 caacgggttc taaatctgca cttaaattta tgtgcagagt atggcgggct atatccggaa
     7981 tagcagtcac cacgccgcga tattcgccca ccgccagact acgggcataa taatcgtcag
     8041 cgaccgtttc cacaccactc actgcacggg cggcgagaaa tcccaacatc cacgaccagt
     8101 catacggcgg ctgccagttc agggtataca tcgcatctcc ttattcatcc gctttcagca
     8161 taaacgttat tcagacgctg cgctttgctt tcatattccg gttgtcgcga cggcaacatt
     8221 tcgctaaagt cacgcccctt cttcaccggc atggggatta tttcgtgttt attggttttg
     8281 attacggtac agcaaactgt tcagtggcgg tcatgcgtga cggtaaaccg cacttgctaa
     8341 aaatggaaaa cgacagcacg ctgctgcctt caatgctttg cgcgccaacg cgtgaagcgg
     8401 taagcgaatg gctgtaccgc catcatgatg ttccggcaga cgacgatgaa acgcaggcgc
     8461 tgttgcgtcg ggcgattcgt tataaccgcg aagaagatat cgatgttacg gcgaaaagcg
     8521 tgcagttcgg tctttcctca ctggcacagt acattgatga tccggaagaa gtgtggtttg
     8581 tgaaatcacc aaaatcgttc ctcggtgcca gcggcttaaa accgcagcag gtagcgctgt
     8641 ttgaggatct ggtctgcgca atgatgttgc acattcgcca gcaggcgcag gcgcagctgc
     8701 cagaagcgat tactcaggcg gtgattggtc gcccgatcaa ctttcagggt ctgggcggtg
     8761 atgaagcaaa cacccaggcg caagggattc tggaacgcgc ggcgaagcgt gccgggttta
     8821 aggatgtggt attccagtac gagccggtag cggcagggct ggattacgaa gcgactttgc
     8881 aggaagaaaa acgggtgctg gtggtggata tcggcggtgg tacgactgac tgttcattgc
     8941 tgctgatggg gccgcagtgg cgttcgcgtc tcgatcgtga agccagcctg ctgggtcaca
     9001 gtggttgccg tattggcggt aacgatctgg atatcgcgct ggcgtttaaa aacctgatgc
     9061 cactgctggg tatgggtggc gaaaccgaaa aaggcatcgc cctgccgatt ctgccgtggt
     9121 ggaatgcggt tgccatcaac gatgtacctg cgcagagtga tttctacagt agtgccaacg
     9181 gtcgtctgct taacgatctg gtacgcgatg cccgcgaacc ggaaaaagtg gccctgctac
     9241 agaaagtctg gcgtcagcgt ttaagctatc gcctggtgcg taacgcagaa gagtgcaaaa
     9301 ttgctctttc aagcgtagcg gaaacccgcg cctcactgcc gtttatcagc gatgaactgg
     9361 ctacgctgat tagccagcaa gggctggaaa gcgccctcag ccagccactg gcgcggattc
     9421 tggaacaggt gcaactggcg ctggataacg cccaggaaaa accggacgtt atctacctga
     9481 ccggcggaag cgcccgttct ccgctgatta aaaaagcgct ggcagaacag ttgccgggca
     9541 ttccgattgc aggcggcgat gactttggct ccgtcaccgc cgggctggca cgctgggcgg
     9601 aagtggtgtt tcgttaattg aaaaatggct cagcagcggc ttaaatctgc ctgcgcctga
     9661 gccagttttt ctgccgcctg acgtaacggt tccatcaacg gcatggtccg tgctggagca
     9721 tgaaggcgga atcgctgcaa ctctgtcgcg ccgacagtca acgccgattc cagccgatgt
     9781 cgtttggcgg tcattttcgc cattaccgct tgtctgtctg ctggcgttac cgcttcattc
     9841 gtcctgaaca caaaacggcg ttcgcaactt gctttccagt cgataaccgc ctgtgtcaga
     9901 tggtcgccaa accctttcac ttgcttaacg ccacgacggg taacatctgc tgctgtttca
     9961 ataccaaagg accgtaacgc cgctttacgc gcagggccaa cgccgggaat agaggcagca
    10021 tcaataaaaa atccctccag aaacttctgc ttctgccgtt cccttgcagt gtcatgaagt
    10081 gctgccagag cgcgtttttc ttcttccggt aacccgagca tttcgtcctt cattttttcg
    10141 agcatcgtcc gtttggcgat aaaaccttcc agcccgccca actgttggat ctggctgact
    10201 aaatggtcat agtccatttt cgcgcgatta aatgcctcac ggcgttgctg gatttctgct
    10261 ttataagctt tacttgtcag actgccaata atccagatag ccgccagcac aggaaccaaa
    10321 ataatataac gcggttctgc ctgaaggccg caaagcagca gcgataacgc tgacagcgcg
    10381 atctcaatca gaatgatgta ttcgcgccgt aacaggcctg aaggaagtgg cctgccagtc
    10441 ggttggaaat gatcgggtaa tggtagttgc aatgctggcg gtgctactga tgccatcacc
    10501 atcgcccaga ctttcgccag cacaaaatcc ccgccggtgg taatgacctc ttcgccgaga
    10561 tcaataaaat agataacgcc ttgattatcc agcgtacacc acgggcagtc agtcaaatga
    10621 gcggggtaaa catgcattgc tgaaacggta catttcttta actgttggcg tagagagtcc
    10681 agtgccgcta cccacgcttt agccgtcgga cgccccgttg ccacgccact ttccgtgaat
    10741 gcctgctgaa acatggcttc aacatcgccc ggtaacatcg acagcggaat agatcgtggc
    10801 ggcggtttta aaccacgtcg ctgattatct gacgcgtagg cataacggaa atgggcaata
    10861 tccgtctcca gcgcattacc cgcatcagag ataagcggca caccagaata aggatgccgc
    10921 ccaccaaaca agacgtgaaa aatcagcaac gcaaggccaa aattatcgtg attcgcggta
    10981 cgttcaaaac cgacaaatga tggcaaggtt tgtagctctg gcggcgtaaa atgcgacacg
    11041 ccgacttcgc ataaatgcag tgtgccattg gcgttaatct gaaaggagtc gctgtcgatc
    11101 aacaccactt tgctgtcgcg acctaccata aagctgttct ggtttacgtc acccacgacg
    11161 tgcccgtgct cgtgaaccgt agcaaaagat gaagcaatat tacgcgcaac atagagtaga
    11221 aaatcccacg cacaatgagg gtaactctgg cgacgatgtg ccgggctata gatcatatga
    11281 atcggttctt taccagaaac ttttggcatc ataaaaccga taacttttcc gcctcgtcca
    11341 ccgtgaagcg ttgcctgcgg ccaggcgaca taattcaaca actgcgcgtc agctgtcgca
    11401 gccataaagg caagtttgtc ctgttttaag gcgggtggcg gcgtgtgata aatcttggcg
    11461 acgctatcgg caaactcctc gatatcataa accgcgcctt caccgccttt gcccagttca
    11521 cggcctggcg taacgcactc accagtagca gtatataaag tgggtttcat ggtaaccaca
    11581 gcgccagtgc caacgtttta tcatcatccg tacgctcgtt taccgccggg ctggacaaaa
    11641 actgctttaa caattcaggt aataaatcga gttgttcctg cgttgctgcc gccagtccat
    11701 tgaaaaacgg ggtaaagaaa ggcacatgcg gagaattatc caacatattt aacgccagtc
    11761 gctgaatacc atccgtaaat gctgcaactt tatgcgcacg ctcagtgctg gtgaaagttt
    11821 ccagtctgga aacagcatct tcatcgctaa tgaaatgcgt catattggca tattcaccca
    11881 ccatcggcgt aagcggtaat tgcagaccgt gaccaaaatc caccaccacg ccgccatcgc
    11941 caatctgcat aatcaaggtg ccgttagccg atgaaatcaa tcccagaaac gtgcaggcaa
    12001 aatcgcgaac tgctaattct ttggcttccg cctcggcaaa aagtcgctga cgaacggtca
    12061 gcacaatatc cgtggcgagg atatcattga gtccaaattc accgtgctgt actttttgca
    12121 ccataaacgc catcgcttca ttgacggcga gcttcgcgcc ttctcctccc tgcgaaacgc
    12181 tacctgcgcc atcagcaagg aacatcacca gtaatggctg ctggtcattt agccaggcaa
    12241 cctgcatctg gcaggcatcc tgacaaggta aatcagcgct aatgtgcgac gttccaacag
    12301 cggatgcata aaccagacgc cagctcacac tgacgtccag ccttttggcg cttccagcac
    12361 aacttccgtt cccggcgtgg agcgggaaac tgaacgcagt gaactggata accagctaaa
    12421 cagttcgcgg aattgtaatc cctgcagaga caaaggctga cggacgctga tttgggccag
    12481 cgtcttcata tccgccccct gaacaccgat ggaaaagaag gcaaacttct tatcttcttc
    12541 cccctgaaac actttgttgg cggcagcctg ccactcatcg gttggtgcac catcggtgat
    12601 caggaaaatc cacgggcgat aatacgaaat accatttgca cgatactcac gtttccgctc
    12661 ctctaccata tccagggctt tggtaatggc ggcgcccatt ggtgtatcgc cctgggcaaa
    12721 caggatgggc gggaaaaaat tagcggcgct ggtaaagggt tgttccacat gcaccgggcc
    12781 gaacgtcaca atgccgagtt ccactctttt taaagccagc ggatcagcaa gcagttcatc
    12841 acgaaaggta accaatccgg cgttaagttc attaataggt ctgccgctca tcgagccaga
    12901 tacatccagc agcagaatgc aggggcaacg tggttccggg ttactggcaa aatcgctggt
    12961 ggcaaacgtg atttgttcgc tcataagaaa tcctttctta gagaattatt gaggtccggt
    13021 atcataccga ctgcaataat gaaataagag caaacgaaga aactttcaga attagtcccg
    13081 gcggcgcaga aaattttgct attcgcctcg caagaacgga gagccgtcga ttgacactaa
    13141 gggcggagtg acataatttc aggagtgagg gttagggaga ggtttccccc tccccctggt
    13201 gttcttagta agcctggaag ctaatcacta agagtatcac cagtatgatg acgtgcttca
    13261 tcataaccct ttccttatta aaagccctct tctccgggag agggtttccc gtttcagcgt
    13321 cccgctgaaa tcgtcggctt acctcctttc gccctgaatg cagtctatcg ctaacgcgtg
    13381 aacaaaatag ttccctctgt gattattgcg taacgactcg caaagaatat ttgctggcag
    13441 gatcgcagac tgcaaagcct gcagattgac aatcttatcg tgaaggcata ctttcaggag
    13501 tgagggtaga gcggggtttc ccccgccctg gtagtcttag taagccggga agcttatgac
    13561 taagagcacc acgatgatga tccacttcat catgaccctt tccttattta aggccccttc
    13621 ctcgggaggg gctttcccgt ttcagcgtcc cgctgaaatc gtcggcttac ctcctttcgc
    13681 cctgaattca gtctatcgct aacgcgtgaa ctaaatagtt tcctctgtga ttattgcgta
    13741 acgtctcgca aagaatattt gctggcagga tcgcagactg caaagcctgc ggattgacat
    13801 tctgaacgtg aaggcatact ttcaggagtg agggtagagc ggggtttccc ccgccctggt
    13861 agtcttagta tgctgggtag ctaatcacta agagcaccac gatgataagt atcttcatca
    13921 tgaccctttc cttatttaag cccccttcct cgggaggggc tttcccgttt cagcatcccg
    13981 ctgaaatcgt cggcttacct cctttcgcca tgcatgcagt ctatcgctaa cgcgtagata
    14041 aaatagtttc ctgtgtcatt actggatgca tgctcgcaaa gcgcaccgtc attcagacga
    14101 ttcccgaaac tgtttcataa ttcctccatt tttctccctt attggctggc tacactagta
    14161 tcattccgcg aaacgtttca ggaagagaaa ctcttaacga tgaaaggcag ttataaatcc
    14221 cgttgggtaa tcgtaatcgt ggtggttatc gccgccatcg ccgcattctg gttctggcaa
    14281 ggccgcaatg aaccccagag cgcagcacca ggtgcgacga aacaagcgca gcacccacct
    14341 gcgggggggc gccgtggtat gcgttctggc cctttagcgc ctgttcaggc agcgactgcc
    14401 gtggaacagg cggttccgcg ttatctcacc gggcttggca ccatcaccgc ggctaacacc
    14461 gttacggtgc gcagtcgcgt ggacggccaa ctgatggcgt tgcatttcca ggaaggccag
    14521 caggtcaaag caggcgattt actggcagaa attgacccca gccagtttaa agttgcatta
    14581 gcacaagccc aggggcaact ggcaaaagat aaagccacgc ttgccaatgc ccgccgtgat
    14641 ctggcgcgtt atcagcaact ggcaaaaacc aatctcgtat ctcgtcagga actggatgcc
    14701 caacaggcgc tggtcagtga aaccgaaggc accattaaag ccgatgaagc gagcgtcgcc
    14761 agcgcacaac tgcaactcga ctggagccgg attactgcgc cagtcgatgg tcgtgttggc
    14821 ctgaagcagg ttgatgttgg taaccaaatc tccagtggcg acacgaccgg gattgttgtg
    14881 atcacccaga cgcatcctat cgatttgctc tttactctgc cggaaagcga tatcgctacc
    14941 gtagtgcagg cgcaaaaagc cggaaaaccg ctggtggtag aagcctggga tcgcaccaac
    15001 tcgaagaaat taagcgaagg cacgctgtta agtctggata accaaatcga tgccactacc
    15061 ggtacgatta aagtgaaagc acgctttaat aatcaggatg atgcgctgtt tcccaatcag
    15121 tttgttaacg cgcgcatgtt agtcgacacc gaacaaaacg ccgtggtgat ccccaccgcc
    15181 gccctgcaaa tgggcaacga aggccatttt gtctgggtac tgaatagcga aaacaaagtc
    15241 agcaaacacc tggtgacgcc tggcattcag gacagccaga aagtggtgat ccgcgcaggt
    15301 atttctgcgg gcgatcgcgt ggtgacagac ggcattgatc gcctgaccga aggggcgaaa
    15361 gtggaagtgg tagaagccca gagcgccacc gcaccggaag agaaagccac cagccgcgaa
    15421 tacgcgaaaa aaggagcacg ctcctgatgc aggtgttacc cccgagcagc acaggcggcc
    15481 cgtcgcgcct gtttattatg cgtcctgtgg ccaccacgct gctgatggtg gcgatcttac
    15541 tcgccgggat tatcggttat cgcgccctgc ccgtttcggc gctgccggaa gtggactatc
    15601 cgaccattca ggtggtcacg ctctacccag gtgcgagccc ggatgtcatg acctctgccg
    15661 ttaccgcgcc gctcgaacgc cagttcgggc agatgtctgg tctgaaacag atgtcgtcgc
    15721 aaagttccgg cggtgcgtca gttatcactt tgcagttcca gctaacattg ccgctggatg
    15781 tcgccgagca ggaagttcag gccgcgatta acgccgccac taacctgcta cctagcgatc
    15841 tgcctaaccc gccggtttac agcaaagtga acccggcaga tccgccgatt atgacgctcg
    15901 ccgtcacctc aaccgccatg ccgatgaccc aggtagaaga tatggtggaa acccgcgtcg
    15961 cgcagaagat ctcgcagatt tccggcgtcg gcctggtgac actttccggc ggccagcgtc
    16021 cggctgttcg cgtcaaactt aacgctcagg cgattgccgc cctcggcctg accagcgaaa
    16081 ccgtgcgcac cgccattacc ggtgctaacg ttaactcggc aaaaggtagc ctcgacggcc
    16141 cttcccgcgc ggtcacgctt tccgcgaatg accagatgca atccgccgaa gagtatcgcc
    16201 agttgattat cgcctaccag aacggcgcgc cgattcgtct gggcgatgtc gcaaccgtag
    16261 agcaaggggc agaaaacagc tggctcggcg cgtgggcgaa caaagaacag gccattgtga
    16321 tgaatgttca gcgccagccc ggtgctaaca ttatctccac cgccgacagc atccggcaga
    16381 tgctgccgca gcttaccgag agtctgccga aatcggtgaa ggtgacggtg ctttctgacc
    16441 gcaccaccaa tatccgagca tccgtcgacg atacccagtt tgaattgatg atggctatcg
    16501 cgctggtagt catgattatc tacctgtttt tgcgcaatat tccggcgacc atcattcccg
    16561 gtgtcgctgt gccgctgtcg ttaatcggca ccttcgcggt tatggtgttt ctcgattttt
    16621 caatcaataa cctgacgctg atggcgttaa ctatcgccac cgggttcgtg gtcgatgacg
    16681 ccatcgtagt tatcgaaaat atttcccgct atatcgaaaa aggcgaaaaa ccgttggcgg
    16741 cggcgctcaa aggcgcgggt gaaatcggct ttaccattat ctcgctgacc ttctcactga
    16801 ttgcggtgtt gatcccactg ctgtttatgg gcgatatcgt cgggcgcctg ttccgcgaat
    16861 ttgcgattac tctggcagtc gcgattttga tctcagcggt ggtgtcgctg accctgacgc
    16921 cgatgatgtg cgcgcggatg ctcagccagg agtcgctgcg taaacagaac cgcttctccc
    16981 gtgcctcgga aagaatgttc gacaggataa tcgccgccta tggtcgtgga ctaacgaaag
    17041 tgctgaatca tccgtggctg accttaagcg tggcgctcag cacgctgctg cttagcgttc
    17101 tattgtgggt gttcattccg aaaggtttct tcccggtaca ggataacggc attattcagg
    17161 gcactttgca ggcaccgcaa tccagctcct ttgccaatat ggcccagcgg caacgccagg
    17221 tcgcggacgt gattttgcag gatccggcag tgcaaagtct gacctcattt gtgggcgttg
    17281 atggcactaa cccgtcgctg aacagtgcgc gtttacagat caacctcaaa ccgctggatg
    17341 aacgtgatga tcgggtgcaa aaagtcatcg cccgtctgca aacggcggtg gataaagtgc
    17401 cgggcatcga tctcttcctg caaccaacgc aggatctgac tattgatact caggtcagcc
    17461 gcacccagta ccagtttacc ttgcaggcca cctcactgga tgcgctcagt acctgggtgc
    17521 cacagttgat ggaaaaactc cagcagctgc cacagctttc tgatgtctcc agcgactggc
    17581 aggacaaagg gctggtggcg tatgtcaatg ttgatcgcga cagcgccagc cgtctgggga
    17641 tcagtatggc ggacgtcgat aacgccctgt acaacgcgtt tggtcagcgg ctgatttcca
    17701 ctatttatac tcaggccaac cagtatcgcg tggtgctgga gcacaacacc gaaaataccc
    17761 cagggcttgc ggcgctggat actattcgcc tgaccagcag cgatggtggc gtggtaccgc
    17821 taagctcaat tgccaaagtt gaacagcgtt ttgcgccgct ttccatcaac catctggatc
    17881 agttcccggt aacgaccatc tcctttaacg tgccggataa ctattcgctg ggcgatgcgg
    17941 tgcaggcgat tatggacacc gaaaagacgc tgaatctgcc ggtggatatc accacgcaat
    18001 ttcagggcag caccctcgcc ttccagtcgg cgctgggcag cactgtctgg ctgattgtcg
    18061 cggcggtggt ggcgatgtat atcgtgctcg gcattctgta cgagagcttt attcacccga
    18121 tcaccattct ctcgacgcta cccaccgcag gggtcggcgc gctgctggca ttaatgattg
    18181 ccggtagcga actggatgtg attgcgatta tcggcattat tttgctgatc ggtattgtga
    18241 agaagaacgc catcatgatg atcgacttcg cgctggctgc tgagcgcgag caaggcatgt
    18301 cgccgcgcga tgcaatctac caggcttgtc tgttgcgttt tcgtccgatc ctgatgacca
    18361 ccctggcggc tctgcttggc gcgctgccgc tgatgttgag taccggggtc ggcgcggaac
    18421 tgcgtcgtcc gttaggtatc ggcatggtcg gcggtctgat tgtcagccag gtgctgacgc
    18481 tgttcaccac gccggtgatt tatctgctgt tcgaccgcct ggcattgtgg accaaaagtc
    18541 gctttgctcg ccatgaagag gaggcgtaag tgaagttttt tgccctcttc atttaccgcc
    18601 cggtggcgac gattttactg tcggtcgcca ttaccctgtg cggcatactg ggcttccgta
    18661 tgctgccggt cgccccgctg ccgcaggtcg attttccggt gattatggtc agcgcctcgc
    18721 tgcccggtgc gtcaccagaa acaatggcgt cttccgttgc cacgccgctg gagcgctcac
    18781 ttgggcgcat tgccggagtc agtgaaatga cctccagcag ttcgctcggc agcacgcgta
    18841 ttattttgca gtttgatttt gaccgggata tcaacggcgc ggcgcgcgat gtgcaggcgg
    18901 cgatcaacgc tgcacaaagt ttgctgccca gcgggatgcc cagccgtccg acctatcgca
    18961 aagctaaccc gtcggatgcg ccaattatga tcctcacgct gacatccgat acttattcgc
    19021 agggtgaact gtacgatttt gcctcgacgc agctggctcc gacgatttcg caaatcgacg
    19081 gtgtcggtga tgtcgatgtt ggcggcagct cgctgcccgc cgtacgcgtc gggctgaatc
    19141 cgcaggcgct gtttaatcag ggcgtctcgc tggacgacgt acgcaccgcc atcagcaatg
    19201 ccaacgtgcg taaaccgcag ggggcgctgg aagatggcac tcaccgctgg cagatccaga
    19261 ccaatgatga gctaaaaacc gccgccgaat atcagccgtt gattattcat tacaacaacg
    19321 gcggcgcggt tcgtctgggc gatgtggcga cggtgaccga ctcggtgcag gatgtgcgca
    19381 acgccgggat gaccaacgcc aaaccggcta ttttgctgat gatccgcaag ctgccggaag
    19441 ccaatattat ccagacggta gacagcatcc gggcaaaatt accggagttg caggaaacca
    19501 ttccggcggc gattgatctg caaattgctc aggatcgctc ccccaccatt cgcgcctcgc
    19561 tggaagaagt cgagcaaacg ctgattatct cggtggcgct ggtgattctg gtggtgtttt
    19621 tattcctgcg ctcgggtcgc gccactatta ttcccgccgt tgcggtgccg gtttcgctga
    19681 ttggtacgtt tgcggcgatg tacctgtgcg gattcagtct caataacctt tcgttaatgg
    19741 cgctcaccat cgctactggt ttcgtggtgg atgacgccat cgtggtgctg gaaaacattg
    19801 cccgtcatct ggaagcgggg atgaaaccgc tacaggccgc gctacaaggt actcgcgaag
    19861 tcggctttac ggtgctgtcg atgagtctgt cactggtggc ggtgttcctg ccgctgctgt
    19921 tgatgggcgg attgccgggc cgactgttac gcgaatttgc cgtgacgctt tctgtcgcca
    19981 ttgttatatc gttgctggtt tctctgacat taacgccaat gatgtgtggc tggatgctga
    20041 aagccagcaa gccgcgtgag caaaagcgac tgcgtggttt tggccgcatg ttggtagccc
    20101 tgcaacaagg ctacggcaag tcgctgaaat gggtgctcaa tcatacccgt ctggtgggtg
    20161 tggtgctgct tggcaccatt gcgctgaata tctggctgta tatctcgatc ccgaaaacct
    20221 tcttcccgga gcaggacacc ggcgtgctga tgggcgggat acaggcggat cagagcattt
    20281 cgtttcaggc gatgcgcggc aaattgcagg atttcatgaa aattatccgt gacgatccgg
    20341 cagtggataa tgtcaccggc tttaccggcg gttcacgagt taacagtggg atgatgttta
    20401 tcaccctcaa gccacgcgac gaacgcagcg aaacggcgca gcaaattatc gaccgtctgc
    20461 gcgtaaaact ggcgaaagaa ccgggggcga atctgttcct gatggcggta caggatattc
    20521 gcgttggtgg gcgtcagtcg aacgccagct accagtacac gttgttatcc gacgacctgg
    20581 cggcactgcg agaatgggag ccgaaaatcc gcaaaaaact ggcgacgttg ccggaactgg
    20641 cggacgtgaa ctccgatcag caggataacg gcgcggagat gaatctggtt tacgaccgcg
    20701 acaccatggc acggctggga atcgacgtac aagccgccaa cagtctgtta aataacgcct
    20761 tcggtcagcg gcaaatctcg accatttacc agccgatgaa ccagtataaa gtggtgatgg
    20821 aagtggatcc gcgctatacc caggacatca gtgcgctgga aaaaatgttc gttatcaata
    20881 acgaaggcaa agcgatcccg ctgtcgtatt tcgctaaatg gcaaccggcg aatgccccac
    20941 tatcggtgaa tcatcaggga ttatcggcgg cctcgaccat ttcgtttaac ctgccgaccg
    21001 gaaaatcgct ctcggacgcc agtgcggcga tcgatcgcgc aatgacccag cttggggtgc
    21061 cttcgacggt gcgcggcagt tttgccggca cggcgcaggt gttccaggag acgatgaact
    21121 cgcaggtgat cctgattatc gccgccatcg ccacggtgta tatcgtgctg ggtatccttt
    21181 acgagagtta cgtacatccg ctgacgattc tctccaccct gccctcggcg ggcgttggag
    21241 cgctgttggc gctggagctg ttcaatgccc cgttcagcct aatcgccctg atagggatca
    21301 tgctattaat cggcatcgtg aagaaaaacg ccattatgat ggtcgatttt gcgcttgaag
    21361 cccaacggca cggtaacctg acgccgcagg aagctatttt ccaggcctgt ctgctgcgtt
    21421 ttcgcccgat tatgatgacc accctggcgg cgctgtttgg tgcgctgccg ctggtattgt
    21481 cgggcggcga cggctcggag ctgcggcaac ccctggggat caccattgtc ggcggactgg
    21541 taatgagcca gctccttacg ctgtatacca cgccggtggt gtatctcttt ttcgaccgtc
    21601 tgcggctgcg tttttcgcgt aaacctaaac aaacggtaac cgagtaaatg acagatcttc
    21661 ccgacagcac ccgttggcaa ttgtggattg tggctttcgg cttctttatg cagtcgctgg
    21721 acaccaccat cgtaaacacc gcccttccct caatggcgca aagcctcggg gaaagtccgt
    21781 tgcatatgca catggtcatt gtctcttatg tgctgaccgt ggcggtgatg ctgcccgcca
    21841 gcggctggct ggcggacaaa gtcggcgtgc gcaatatttt ctttaccgcc atcgtgctgt
    21901 ttactctcgg ttcactgttt tgcgcgcttt ccggcacgct gaacgaactg ttgctggcac
    21961 gcgcgttaca gggcgttggc ggcgcgatga tggtgccggt cggcagattg acggtgatga
    22021 aaatcgtacc gcgcgagcaa tatatggcgg cgatgacctt tgtcacgtta cccggtcagg
    22081 tcggtccgct gctcggtccg gcgctcggcg gtctgctggt ggagtacgcg tcgtggcact
    22141 ggatcttttt gatcaacatt ccggtgggga ttatcggtgc gatcgccaca ttgatgttaa
    22201 tgccgaacta caccatgcag acgcggcgct ttgatctttc cggcttttta ttgctggcgg
    22261 ttggcatggc ggtattaacc ctggcgctgg acggcagtaa aggtacaggt ttatcgccgc
    22321 tggcgattgc tggcctggtc gcagttggcg tggtggcact ggtgctttat ctgctgcacg
    22381 ccagaaataa ccaccgcgct cttttcagtc tgaaactgtt ccgcactcgt accttttcgc
    22441 tgggcctggc ggggagcttt gccggacgta ttggcagtgg catgttgccc tttatgacac
    22501 cggttttcct gcaaattggc ctcggtttct cgccttttca tgccggactg atgatgatcc
    22561 cgatggtgct tggcagcatg ggaatgaagc gaattgtggt acaggtggtg aatcgctttg
    22621 gttatcgtcg ggtactggta gcgaccacgc tgggtctgtc gctggtcacc ctgttgttta
    22681 tgactaccgc tctgttgggc tggtactacg ttttgccgtt cgtcctgttt ttacaaggga
    22741 tggtcaactc gacccgtttc tcctccatga acaccctgac gctgaaagat ctcccggaca
    22801 atctggcgag cagcggcaac agtctgctgt cgatgattat gcaattgtcg atgagtatcg
    22861 gcgtcaccat cgccgggctg ttgctgggac tttttggttc gcagcatgtc agcgtcgaca
    22921 gcggcaccac acaaaccgtc tttatgtaca cctggcttag catggcgttt atcatcgccc
    22981 ttccggcgtt catctttgcc agagtgccga acgatacgca tcaaaatgta gctatttcgc
    23041 ggcgaaaaag gagcgcgcaa tgaagttctg gcgacccggt attaccggca aactgtttct
    23101 ggcgattttc gccacctgca ttgtcttgct gatcagtatg cactgggcgg tgcgtatcag
    23161 ttttgagcgt ggctttattg attacatcaa gcatggtaat gaacagcgat tacaactgtt
    23221 aagtgatgcg cttggcgagc agtatgcgca gcatggcaac tggcgcttcc tgcgcaacaa
    23281 tgatcgcttt gtctttcaga tcctgcgttc atttgaacac gataattcgg aagataaacc
    23341 cggcccgggt atgccaccgc acggctggcg tacccagttc tgggtggttg atcaaaacaa
    23401 caaagtgctg gttggtccgc gagcgccgat tccacctgac ggtacacggc gacccattct
    23461 ggtcaacggt gcggaagttg gcgcggtgat cgcctccccc gttgagcggt taacgcgcaa
    23521 tactgatatc aatttcgata aacaacagcg gcaaaccagc tggttgattg tcgctctggc
    23581 aacgttactc gcggcgctcg ccacctttct gctggcgcgc ggtttgctgg caccggtaaa
    23641 acgacttgtc gatggcacgc acaaactggc ggcgggcgat ttcactaccc gcgtaacgcc
    23701 caccagtgaa gatgaactgg gcaaactggc gcaagacttc aaccagctcg ccagcacgct
    23761 ggagaaaaac cagcaaatgc ggcgcgattt tatggccgat atttctcacg aactgcgtac
    23821 gccattagcg gtgctgcgcg gtgaactgga agccattcag gatggcgtgc gcaaattcac
    23881 gccggagacg gtggcgtctt tacaggcgga ggtcggtaca ctgaccaaac tggttgacga
    23941 tcttcatcag ttgtcgatgt ctgatgaagg cgctctcgcc tatcaaaaag caccggtaga
    24001 tttgatccca ctgctggaag tggcgggcgg cgcatttcgc gaacgattcg ccagccgtgg
    24061 cctgaaactg caattttccc tgccagacag tattaccgta tttggcgatc gcgaccgttt
    24121 aatgcagtta ttcaataact tacttgaaaa cagcctgcgc tatactgaca gcggcggcag
    24181 cctgaaaatc tctgccgagc aacacgacaa aacggtgcgc ctgacctttg ccgacagtgc
    24241 gccaggtgtc agtgacgatc agctacaaaa attgtttgaa cgtttttatc gcaccgaagg
    24301 ttcccgcaac cgtgccagcg gcggttccgg gctggggctg gcgatttgcc tgaacattgt
    24361 tgaagcacat aatggccgca ttattgccgc ccattcgcct tttggcgggg taagcattac
    24421 agtagagtta ccgctggaac gggatttaca gagagaagta tgaccgagtt accaatcgac
    24481 gaaaacacac cgcgtatttt gatcgtggaa gatgaaccga agctggggca gttgctcatt
    24541 gattatctgc gtgctgcgag ctatgcgccg acgcttatca gtcacggcga tcaggtactg
    24601 ccgtatgtgc gccagacacc accggatctg atcctgttgg atctgatgct ccctggcacc
    24661 gatggcctga cgctgtgccg ggaaattcgt cgtttttctg acattcctat cgtgatggtg
    24721 acagcaaaaa tcgaagagat cgaccgcctg ctggggctgg agattggcgc agatgactac
    24781 atctgcaagc cgtacagccc acgggaagtg gtagcgcgtg tcaaaaccat tttgcgccgt
    24841 tgcaaaccgc agcgtgagtt gcaacaacag gatgctgaaa gcccgttgat tatcgacgaa
    24901 ggccgttttc aggcttcatg gcgcggtaaa atgcttgacc tgacgcctgc ggaatttcgt
    24961 ctgctgaaaa cgctctctca tgaaccagga aaagtgttct cccgcgagca attgctcaat
    25021 catctttatg acgactaccg cgtagtaacc gaccgcacca tcgacagcca cattaaaaac
    25081 ctgcgccgca agctggaatc tctcgacgcc gaacagtcat ttatccgcgc cgtttacggc
    25141 gttggttacc gctgggaagc cgacgcctgc cgcatcgttt agttttagcg acatgacttt
    25201 gttagccgga gatggcgttc agacccgatc cggcaacatt atcccacgca tggtcagcaa
    25261 actgacatta tctctccgcc ctggcaggca tcagaaaatc gctcatactt taatcggtaa
    25321 acagcacctt tagatgctgt tttcgataca caatttcaat caaggagtca ttatggctgg
    25381 ttggtttgaa ctcagtaaga gcagtgataa tcagttccgg tttgtgctaa aagcgggcaa
    25441 tggtgagact atcctcacca gcgagctgta tacttcaaaa gcctctgcgg aaaagggcat
    25501 cgcgtcggtg cgtagcaaca gcccgcaaga agaacgctat gagaaaaaaa cggcaagcaa
    25561 cggcaaattc tatttcaatc tgaaagccgc caatcatcaa attatcggct ccagccagat
    25621 gtatgccacc gcgcaatctc gtgaaaccgg aattgcctcc gttaaagcca atggcacaag
    25681 ccagacggtg aaagataata cgtaattacg atgccgggcg tgggttgttg gcgtccggct
    25741 ttatttccaa ataacgctta tttatttacc tgcgccatta aaatagtgga cactgatgaa
    25801 tactcattgt gctttctttg tttaacatct ccatgcttat cctcctgaac atgttcatct
    25861 gccaaaatat cttctaatac ttttcgtgac gctatccggt ttatgacata cactgatgta
    25921 agatttttac ttacaggaga gcataaacat ggccagaacg atgacggttg atctcggcga
    25981 tgagttgcgt gaatttatcg agtccttaat tgaatccggc gactatcgca cccaaagcga
    26041 agtgatccgt gagtcactgc gcttacttcg agaaaagcaa gcagagtctc gcctgcaagc
    26101 attacgtgac atgcttgcag aagggttgag tagtggtgaa gctcagccgt gggaaaagga
    26161 cgcgttttta cggaaggtga aagcagggat aaggaaataa tgtgcgtatt attaaactca
    26221 tgccaaaagc caatgaggat ttagaaggca tctggtatta cagttatcac catttcggcg
    26281 aaccacaagc cgacagatat gtggaacatc tttcagacgt tctccagatt ctgagtaaca
    26341 ataatatagg tacgcccaga cctgaattag gcgaaggtat ttttgttctt ccttttgaac
    26401 gccacgtcat ttatttcctg caatcaccag gggaaattat cgtcattcgt atccttaacc
    26461 aaaaccagga cgccaccaga catttacact ggagctaaac ccgccgattg cgctacaatg
    26521 cccgccctta aagtgggggc actcccctaa ccgcttcatc aggtgaagcg gatctgacct
    26581 gtcatcagaa cgagagaatt atgtttaaac cggaactcct ttccccggcg ggaacgctga
    26641 aaaatatgcg ttacgctttc gcttatggcg cagatgctgt ttatgcgggc cagccgcgtt
    26701 actccctgcg tgtgcgcaac aacgaattca accacgaaaa ccttcagctc ggcatcaatg
    26761 aagcccacgc gctggggaaa aagttttatg tcgtggtcaa cattgcaccg cacaacgcca
    26821 agctgaaaac ctttatccgt gacctgaaac cggtggtgga aatggggccg gatgcgctga
    26881 ttatgtccga tccagggctg attatgctgg tgcgtgagca cttccctgaa atgccgatcc
    26941 atctctcggt acaggctaac gccgtgaact gggcgacggt gaaattctgg cagcaaatgg
    27001 gcctgacccg cgtgatcctc tctcgcgagc tgtcgctgga agagattgaa gagatccgca
    27061 atcaggtgcc ggatatggag atcgagatct tcgttcacgg cgcactgtgc atggcctact
    27121 ccggtcgctg cctgctctct ggctatatca acaagcgcga tccgaaccag ggtacctgca
    27181 ccaacgcctg ccgctgggag tacaacgtcc aggaagggaa agaagatgac gttggcaaca
    27241 tcgtacacaa gtacgagccg attccggtgc aaaatgttga gccgacgctg ggtatcggcg
    27301 cgccaaccga caaagtgttt atgatcgaag aagcccagcg tccgggcgag tatatgaccg
    27361 cgtttgaaga tgagcacggc acttacatca tgaactcgaa agatctgcgc gctatcgccc
    27421 atgttgaacg cctgaccaaa atgggcgtgc attcgctgaa aatcgaaggc cgtaccaaat
    27481 ctttctacta ttgcgcacgc accgcacagg tttaccgcaa agctattgat gacgccgctg
    27541 cgggcaaacc gtttgacacc agcctgctgg aaaccctgga aggcctggcg catcgcggtt
    27601 acaccgaagg attcctgcgt cgtcatactc acgacgatta tcagaactac gaatacggtt
    27661 attcggtttc tgaccgccag cagtttgttg gtgagtttac cggtgagcgc aagggggacc
    27721 tcgcggcggt agcggtgaaa aataaattct ccgttggcga cagccttgag ctgatgacgc
    27781 cgcaaggcaa cattaacttt acccttgagc acatggaaaa cgccaaaggt gaagcgatgc
    27841 cggtcgcacc aggcgatggt tatactgtgt ggctcccggt cccacaggat cttgagctca
    27901 attacgcgct gctgatgcgt aatttctccg gggaaagcac gcgtaacccc cacggtaagt
    27961 gattaatttc gattattttt cccggatgga aaattcttag aaaccgatca catacagctg
    28021 catttattaa ggttatcatc cgtttcgctg aaaaacataa cccataaaat gctagctgta
    28081 ccaggaacca cctccttagc ctgtgtaatc tcccttacac gggcttattt tttatgcata
    28141 agccctatcc ctggtcaccg tcttccattg accacatcga tagaatctcc cttcatagca
    28201 cgatgccttt cacgtaacgg catcgtgctc gcacaggttc cggctaagca caaccagaac
    28261 gcgcatgttt gacgcttacc aaaaaatatt ctcactctcc acatttgaat gtcagacgag
    28321 cgacgccatg taatcctgca ccttctgtct tcaggtcaac tatctgcatt tttttgccct
    28381 gagtaacaca gaaatgggct gcatcatttt ttactatatt ttctgcacca gatattctgc
    28441 ccctggctaa agaagcttcg gcttcggtgt agtattggtt atcgagttta cgctgaatat
    28501 tacttttata tgcaagacca aatttaccga tacttgtctc atcat
//
LOCUS       DAPHCV010000064        28069 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.064, whole genome shotgun sequence.
ACCESSION   DAPHCV010000064 DAPHCV010000000
VERSION     DAPHCV010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.064"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            31..933
                     /locus_tag="RH033_002714"
     CDS             31..933
                     /locus_tag="RH033_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2991879.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFII
                     YGPQLISCTNNNMLIRCLRQDTSIDIECHDILLSAENAEELLVQRKTDLAITLQPVIS
                     RSVICMPLHTIRNSLICSNKHPRITDTSNYEQIIAEEFTLLISKSAGIDEIQMDIDER
                     FINRKVSFRSSSLLTIINSISVTDLLGIVPTELYDLHRDFLKLKEIKLEQPLPAVKLY
                     ISYNKASLNNLVFSRFIDRLNDSF"
     gene            1080..2300
                     /locus_tag="RH033_002715"
     CDS             1080..2300
                     /locus_tag="RH033_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDV2991880.1"
                     /translation="MSIYKIPLPLNILEAAKERITWTLNTLPRICVSFSGGKDSGLML
                     HLTAEIARQMGKKICVLFIDWEAQFSCTINYVQSLREFYADVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFSFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDMPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQQLCNPLYNIMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            2273..2902
                     /locus_tag="RH033_002716"
     CDS             2273..2902
                     /locus_tag="RH033_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDV2991881.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(2903..4063)
                     /gene="ybdL"
                     /locus_tag="RH033_002717"
     CDS             complement(2903..4063)
                     /gene="ybdL"
                     /locus_tag="RH033_002717"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HDV2991882.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTRMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVDQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRIALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALSESRLEILPCEGTYFLLVDYSAVSSLDD
                     VKFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            4172..5260
                     /gene="hcxA"
                     /locus_tag="RH033_002718"
     CDS             4172..5260
                     /gene="hcxA"
                     /locus_tag="RH033_002718"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HDV2991883.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGER
                     AIAAAQTKLPPAFELPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQEYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQKQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTEAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFQA"
     gene            complement(5270..5467)
                     /locus_tag="RH033_002719"
     CDS             complement(5270..5467)
                     /locus_tag="RH033_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HDV2991884.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(5640..7745)
                     /gene="cstA"
                     /locus_tag="RH033_002720"
     CDS             complement(5640..7745)
                     /gene="cstA"
                     /locus_tag="RH033_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HDV2991885.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7926..8339)
                     /gene="entH"
                     /locus_tag="RH033_002721"
     CDS             complement(7926..8339)
                     /gene="entH"
                     /locus_tag="RH033_002721"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HDV2991886.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(8342..9088)
                     /gene="entA"
                     /locus_tag="RH033_002722"
     CDS             complement(8342..9088)
                     /gene="entA"
                     /locus_tag="RH033_002722"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HDV2991887.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(9088..9945)
                     /gene="entB"
                     /locus_tag="RH033_002723"
     CDS             complement(9088..9945)
                     /gene="entB"
                     /locus_tag="RH033_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HDV2991888.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDSLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9959..11569)
                     /gene="entE"
                     /locus_tag="RH033_002724"
     CDS             complement(9959..11569)
                     /gene="entE"
                     /locus_tag="RH033_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HDV2991889.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
                     HNLQDAINHPAGDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVYSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(11579..12754)
                     /gene="entC"
                     /locus_tag="RH033_002725"
     CDS             complement(11579..12754)
                     /gene="entC"
                     /locus_tag="RH033_002725"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HDV2991890.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QSSARRFTRSQSLNVVERQAIPEQATFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSHENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12943..13899
                     /gene="fepB"
                     /locus_tag="RH033_002726"
     CDS             12943..13899
                     /gene="fepB"
                     /locus_tag="RH033_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HDV2991891.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQALLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(13903..15153)
                     /gene="entS"
                     /locus_tag="RH033_002727"
     CDS             complement(13903..15153)
                     /gene="entS"
                     /locus_tag="RH033_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HDV2991892.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QIMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            15264..16268
                     /gene="fepD"
                     /locus_tag="RH033_002728"
     CDS             15264..16268
                     /gene="fepD"
                     /locus_tag="RH033_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HDV2991893.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLYTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            16265..17257
                     /gene="fepG"
                     /locus_tag="RH033_002729"
     CDS             16265..17257
                     /gene="fepG"
                     /locus_tag="RH033_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HDV2991894.1"
                     /translation="MIYVSRRLIITCLLLVSACVVAGIWGLRSGAITLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMAGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAASLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLVADLCAQLLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            17254..18069
                     /gene="fepC"
                     /locus_tag="RH033_002730"
     CDS             17254..18069
                     /gene="fepC"
                     /locus_tag="RH033_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HDV2991895.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEDAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELLERIYGLRCMIIDDPVAGTPLVVPLGRTTPSTAKI"
     gene            complement(18066..19199)
                     /gene="fepE"
                     /locus_tag="RH033_002731"
     CDS             complement(18066..19199)
                     /gene="fepE"
                     /locus_tag="RH033_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HDV2991896.1"
                     /translation="MSSLNIKQGSDAHFPEYPLASPSNNEVDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQALEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSTPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDESALYTSWTLSFTAPTSEEAQKVLAGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKTNVNDVNFTPFKYQLRPSLPVKKD
                     GPGKAIIVILSALIGGIVACGDVLLRHAMASRKQDAIMADHLV"
     gene            complement(19415..23296)
                     /gene="entF"
                     /locus_tag="RH033_002732"
     CDS             complement(19415..23296)
                     /gene="entF"
                     /locus_tag="RH033_002732"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HDV2991897.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALTFELPETIDLRTNIDPHGTAQSLMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLECTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPQLATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPDVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EIIANKQRYAEPTLIQHAERLKMLIAQFAADPVLLCGDVDIMLPEEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPHAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDERLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTSLCYNAPLTPQGSAPLKLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTSV
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTTALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGDEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPNGPMQTAANLDEVCEAHLATLLEHQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTDLFTTIEGNYADAVRLLTTAHSVPFEGKATLFVAERTLQEGMNPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGPIIRATLNR"
     gene            complement(23293..23511)
                     /gene="ybdZ"
                     /locus_tag="RH033_002733"
     CDS             complement(23293..23511)
                     /gene="ybdZ"
                     /locus_tag="RH033_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HDV2991898.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDVVCQPQ
                     SQASCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            complement(23514..24716)
                     /gene="fes"
                     /locus_tag="RH033_002734"
     CDS             complement(23514..24716)
                     /gene="fes"
                     /locus_tag="RH033_002734"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HDV2991899.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFRWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTNVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PETPAKEIIWKSERLKNSRRVWIFTTGDATAGERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEQLKAGQVSAEG
                     LRIVLEAGIREPMIMQANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            24959..27200
                     /gene="fepA"
                     /locus_tag="RH033_002735"
                     /pseudo
     CDS             24959..27200
                     /gene="fepA"
                     /locus_tag="RH033_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
     gene            27375..27995
                     /gene="entD"
                     /locus_tag="RH033_002736"
     CDS             27375..27995
                     /gene="entD"
                     /locus_tag="RH033_002736"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HDV2991900.1"
                     /translation="MKTTHTSLPFVGHTLHFVEFDPASFREQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLAECGLTFSLALTLAFSAKESAFKASKI
                     QAAQGFLDYQIISWNKQQLIIRLEDEQFAVHWQIKEKIVITLCQHD"
BASE COUNT         6743 a         7792 c         7234 g         6300 t
ORIGIN      
        1 tatggaagag acaatacaac ggagtatgtt atggctaatc tttatgatct aaaaaaattt
       61 gacctcaatt tactggtcat atttgaatgt atttatcaac atctcagcat tagtaaagcg
      121 gcagaatcat tatatataac gccctctgct gttagtcagt cattacaacg tttacgtgcg
      181 caatttaatg acccgttatt cattcgctca gggaaaggta tcgcgccgac tacaacaggg
      241 ttaaacctgc atcaccatct tgagaaaaat ctcagaggcc ttgaacaaac gatcaatatc
      301 gttaataagt ctgaactaaa gaaaaatttt attatctacg gtcctcagct tatttcttgt
      361 actaataaca acatgctaat ccgctgtctt agacaagata cttcaatcga tatagaatgc
      421 catgacatcc tgctttcagc tgaaaatgcc gaagaactgt tggttcaacg caaaactgac
      481 ctggcaatta cactgcaacc agtaattagt cgctctgtca tctgcatgcc gttacacact
      541 attcgcaatt cccttatatg tagcaacaaa catcctcgta ttactgatac cagtaactat
      601 gaacaaatta tcgccgaaga attcaccctg ttgatatcta aatcagcagg gattgacgaa
      661 attcaaatgg acatagatga acgttttata aaccgtaaag tttcatttcg cagttcttct
      721 ttgttgacta taattaatag catttctgtt actgacttat taggcattgt gcccaccgaa
      781 ctgtacgatt tacatcgtga ttttttgaag ttaaaagaaa taaaactaga acaacctctt
      841 cctgcggtta aactttatat ttcctataac aaggcttcgt tgaacaacct tgtcttctcc
      901 cgattcatag atcgtttaaa tgatagtttc tgattcatta ccactgacaa ataatatatt
      961 acgcatcaat taattcattg tgtgaatata aaataatgtg ctctatcttg cttcgtttat
     1021 cctataatta gcggattcat ctcacgagtc accccgtaac gttcagagag cgtttttaga
     1081 tgtctattta taaaatcccg cttccactga atattctgga agctgcaaaa gaacgtatta
     1141 cctggacact taatacccta ccccgtatct gtgtttcttt ttcgggtggt aaagattccg
     1201 gattaatgct gcatctgaca gccgaaattg cccgacaaat gggcaaaaaa atctgcgttt
     1261 tgtttatcga ctgggaagcg cagttctcat gcactattaa ctatgttcag tcattacgtg
     1321 agttttacgc cgatgtcatc gaagagtttt actgggttgc gctcccgctt acgacgcaaa
     1381 attccctttc tcaataccaa cccgaatggc agtgctggga acctgatgtc gaatgggtgc
     1441 gtcagccccc gcaagatgcg ataaccgatc ctgacttttt ctccttttac cagcctggca
     1501 tgaccttcga acaatttgtg cgtgagtttg ctgaatggtt ttcacaaaaa cgtccggcgg
     1561 cgatgatgat cggcatccgt gcagatgagt cctacaaccg ttttgtcgcc atcgccagtt
     1621 taaataaaca acgttttgcc gatgatatgc cctggaccac cgccgcacca ggcggtcata
     1681 gctggtacat ttaccccatc tacgactgga aagtggctga tatctggacc tggtatgcta
     1741 accatcaaca gctctgtaac ccactgtata atataatgta tcaggcaggc gttcctctgc
     1801 gtcatatgcg aatttgcgaa ccttttggcc cggagcaacg gcaaggatta tggctctatc
     1861 acgttatcga accggatcgc tgggctgcta tgtgcgcacg agtcagcggg gtaaaaagtg
     1921 gcggcattta cgccggacat gataatcatt tttatggtca tcggaaaatc ctcaagccag
     1981 aacatttaga ctggcaagaa tatgcattat tgctgctcaa tagcatgccg gaaaaaacag
     2041 ctgagcatta ccgcaataaa atcgccattt atttgcactg gtatcagaaa aaaggcatcg
     2101 aagttccaca aacccagcaa ggggacattg gcgcgaaaga tatcccctcc tggcggcgaa
     2161 tatgcaaagt tttactcaat aacgattact ggtgccgggc attatcattt agtcctacga
     2221 aagcgaagaa ctatcagcgt tataacgaac ggataaaagg aaaacgtcag gaatggggga
     2281 tactatgcaa caacgattaa cgcaggattt aacccaattt ctcgccagct tgccggaaga
     2341 tgatcgcatc aaagcgatta atgaaatccg catggcgatc catcaggtta gcccctttcg
     2401 tgaagagcca gtggattgcg ttctgtgggt taaaaacagc caacttatgc ctaatgatta
     2461 caacccaaac aacgtggcac cacccgaaaa aaaactgctg aaaaaatcca ttgaaataga
     2521 tggttttacc caacccattg tggtcacgca tacggataaa aatgccctgg aaatcgtgga
     2581 tggttttcac cgccatgaaa ttggtaaagg ctcatcatcg ttaaaattac gcttaaaagg
     2641 ttatttgccg gtgacctgtc tggaaggtac gcgtaatcaa cgcatcgccg cgactattcg
     2701 tcataaccgc gctcgtggtc gccaccaaat cactgccatg tccgaaattg ttcgtgaact
     2761 cagccagttg ggatgggacg ataataaaat tggcaaagag ctgggaatgg acagcgacga
     2821 agtgctgcgc ctgaaacaaa ttaacggcct gcaagagttg tttgccgacc gtcaatattc
     2881 ccgtgcctgg actgtcaaat aactacagct ggcgcaggcg ttcagctgct gccagcaacg
     2941 tcgattcctt cttggcaaaa cagagacgaa tcagtttatg ggggaaggga tcggcgcaaa
     3001 ataccgacag cgggatcgcc gctacaccgt gctcccgcgt cagccactgg caaaacttaa
     3061 cgtcatctag cgaagaaacc gcgctgtaat ccaccagcaa aaagtatgta ccttcgcacg
     3121 gtaaaatctc cagccgactc tcacttaagg cattcaccag aatatcgcgt ttctggcgat
     3181 aaaagtccgg taacgcaaga taatgctcag gttctgcacg tagcatatcg gcaagcgcca
     3241 actgtgccgg ggtattcacc gaaaaggtca gatactgatg cactttgcgg atctcggcgc
     3301 tgatgggcgc aggcgcaacg caataaccca ctttccagcc ggtcatatga taggtcttgc
     3361 caaacgatga caccgccacc gctcgctcac gcaattgcgg atgcgccagc acgctggcat
     3421 ggccctgttg tgaaaaattg atgtgctcgt agacttcatc gctaatgaca aaaatctcgt
     3481 gcccggcgat cgcctgccac aaagcggcga aatcagcctg ctgccagaca gttgcactgg
     3541 ggttatgcgg ggtgttgagg atcaccagtc gggtgcgctc gcttaacaat gcggcaaatt
     3601 cctgccagtc cacgcgaaaa tgcggtggtt gcagtgcaat acgtttcact attccccccg
     3661 aaagcgcgat ggcgggcgcg taactgtcat agctgggatc aaagcagatc acttcatcac
     3721 cattgcgcac caatgcggta atcgctgcat ataacgcttc cgtcgcccct gccgttacgg
     3781 tgatatcgct atcggcatcc ggttgatagc catacagacg ttccgttttc tgagcaatcg
     3841 cctcgcgcaa ggcctgtacg ccagtcatag gcgcgtattg gtttgccccc tggtcaacat
     3901 ggtacgccag ccgctcctgc aaatagcgcg gaccatcaaa atcaggaaag ccttgcgaca
     3961 ggttaatggc ctggtgttgc tgcgccagcg cgctcatccg ggtgaaaata gtggtgccaa
     4021 gttgtggaag tttgctttgt ggaatcagag ggttatttgt catttttatt gtacctgacg
     4081 aagttgcgtg ttgaaggcac tataacacga tgctagtatt tggcaatcaa gacgtttaga
     4141 tgtctaaata aaacaataag gacaacacaa catgcctcac aatcctatcc gcgtggtcgt
     4201 cggcccggct aactactttt cacatcccgg aagtttcaat cacctgcacg attttttcac
     4261 tgatgaacaa ctttctcgcg cggtgtggat ctacggcgaa cgcgccattg ctgcggcgca
     4321 aacaaaactt ccgccagcgt ttgaactgcc aggggcaaag catattttgt ttcgcggcca
     4381 ttgcagcgaa agcgatgtac aacaactggc ggctgagtcc ggtgacgatc gcagcgtggt
     4441 gattggcgtc ggcggcggcg cactgctcga caccgcgaaa gccctcgccc gccgtctcgg
     4501 tctgccgttt gttgccgttc cgacgatcgc cgccacctgc gccgcctgga caccgctctc
     4561 cgtctggtat aacgatgccg gacaggcgct gcattatgag attttcgacg acgccaattt
     4621 tatggtgctg gtggagccag agattatcct caatgcgccg caagaatatc tgctggcggg
     4681 gatcggtgac acgctggcga aatggtatga agcggtggtg ctggctccgc aaccagaaac
     4741 gttgccgcta accgtgcggc tggggatcaa taatgcgcag gccattcgcg acgtcttgtt
     4801 aaacagtagc gaacaggcgc tgagcgatca gcaaaaacaa cagttaacgc aatcattttg
     4861 cgatgtggtg gatgctatta ttgctggtgg tgggatggtc ggtggtctgg gcgatcgttt
     4921 tacgcgtgtg gcggcggccc atgccgtgca taacggtctg accgttttgc cgcaaaccga
     4981 gaagtttctc cacggcacca aagtcgccta cggaattctg gtgcaaagcg ccttgctggg
     5041 tcaggatgat gtgctggcgc aattaacaga ggcgtatcag cgttttcatc tgccgactac
     5101 gctggcggag ctggaagtgg atatcaataa tcaggcggag atcgacaaag tgattgccca
     5161 caccttacgt ccggtggaat ccattcatta cctgccggtc acgctgacac cagatacgtt
     5221 acgtgcagcg ttcgaaaaag tggaatcgtt tcaagcctga cgtaccaatt tagcagcagc
     5281 gcgcgccgcc ttttccaccg tagcgcgcgt cctgccgctc acggaaaaac tcttcgtagg
     5341 tcatcggcgt ttgatcggga tggttaaccc gcatatgttc gacatagttg tcgtaatcag
     5401 gcataccaat catcagcttc gccgcctgac ctaaatattt tccggctttt gccagtgaat
     5461 caaacatctt ggcctccgct aaccatgttg ctgaaagagt gccggatgcg gcgtgaacgc
     5521 tttatccggc ctacaaattc tatcaacgcg ctcggtcagt cccctcgccc catcggggag
     5581 agggttaggg tgagggggaa ccctcggcac tatgccaaca ttcccccctc actctgactt
     5641 tagtgcgcgc cttttgcctg cgccacgatc tcctcgacat tttccggcat tggctcatat
     5701 ggcgtttctt tcgccgttgg cttcggctct ttcaatgccg ccagtgccgt cttaatcgag
     5761 aacagtgcca gaaccaccac gaccaccata aagaagatgg ttaacccggc atcaagacgg
     5821 ttgttgaaca ccagttgcgc cagctgtgac tcagtatact gcgatggaat attgccgctg
     5881 tcgatcattg cctggaactt gttagcaatc gccaggaagc cgactttcgc atccgggcta
     5941 aacgctttct gccagcctgc ggtcagggta caaatcagca gccaggccgt tggtaccagc
     6001 gccacccagg cgtaacgttg acgtttcatt ttgaacagca ccacagcaca gagcatcagc
     6061 gccatccctg ccagcatctg gttggcaata ccaaacagcg gccacaaggt gttaataccg
     6121 cccagcggat cgaccacgcc ctgatgcagg aagtagcccc acgccagcac gcacagcgcc
     6181 gttgccagca ggttagcagg cagtgaatcg gtccgtttca ggcccggaga caccacgccc
     6241 agcagatcct gcaacataaa gcgcgcagca cgcgtacctg catccaccgc cgtcagaata
     6301 aacagtgctt caaacagaat ggcgaagtga taccagaacg ccacatccat catgccgccc
     6361 agcgcaccgt gcagaatgta ggccatcccc accgccaggg tcggcgcacc gcccgcacgg
     6421 gaaatgatcg actgttcacc cacttcgctg gcaatctggt ttagcgtatc cggggtaatc
     6481 gcaaagcccc agctactcac cacctgcgcg gcagaagcaa ccacatccgc cgtccctgcc
     6541 ggagccagca ccgccatcgg gctgttcatg gcgaagtaca cacccggatc gatgatacag
     6601 gcggaaacca gcgccataat cgccacgaag gattccatca acatcccacc gtagccgata
     6661 aagcacgcct gcccttcgtt cgccagcatc ttcggtgtag tgccggaaga gatcagtgca
     6721 tggaagccag acaccgcgcc acaggcgatg gtgataaaca ggaacgggaa caggttaccg
     6781 gtccataccg ggccagtgcc atcaacaaat ttggtcagtg ccggcatggt cagcgtcggg
     6841 cgcataatca aaatgcccac cgccagaccg acgatagtcc cgattttcag gaaggttgag
     6901 aggtagtcac gcggagccag cagcagccac accggcagca ccgccgccac aaaaccgtaa
     6961 cccaccagca tccaggtcag ctgcacgccg gtaaagtcaa agtacggtgc ccaggtcgga
     7021 ctttctgcca cccagccgcc agagataatg gcgaaaatca ggaacaccag gccaatcacc
     7081 gacacttcgc caatacgccc cggacgcaga tagcgcaggt agatccccat aaacagcgcc
     7141 agcggaatag tgaacgcaac ggtatacgtt ccccacgggc tatgggtcag agctttcacc
     7201 acgatcatcg ccagcacggc aaggataatg accatgatca taaagcaggc caccagcgct
     7261 atcaccccgg cggttggccc catctcttct ttgaccagct cacccagcga gcgaccatcg
     7321 cggcgcgtag aaacaaacag caccatgaaa tcctgcaccg caccggcgag aaccacccca
     7381 gcgagcagcc agatcatccc tggcaggtaa cccatttgcg ccgccagtac cggcccaacc
     7441 aacggacctg ctccggcaat ggccgcaaaa tggtgaccga acagcacttt tttgtccgtc
     7501 ggcacatagt ccagcccgtc gttatggcgc accgctggcg tcatacgcgt cgggtcaacc
     7561 gccagcacat ttttggcgat atacagccca taaaaacggt aagcgatcag atagatacag
     7621 accgacgcca ccacaatcca cagcgcgttg atctgttccc cacgatttaa agcaatgtat
     7681 cccagagcaa atgctcccat tacagagagc actgtccaga cgaggtattt ccctgatttg
     7741 ttcatagttg ttatccgtgt gcgtgtccat agagatgtta cattttgttt ctataacatc
     7801 tttgtacaat ttaacaacac agcaaccatg taaatcagag ttaaaaccaa tatttaactt
     7861 aacaacgtta actcgatcac tccgttaacc gaattttcct gcaatctcat tttattctgt
     7921 cgggttcatc ccaaaaccgc tgtgcccagc cgacaggtgc agcaacgccg cccctgttca
     7981 tcgaaaacga cgatttccca gctctgattt tgtcgtccaa gatgcagcgg ctggcagaca
     8041 ccgcgcacct ttccctcaga caccgggcga tgatgtgtgg cgttaagttc cgtgcccacc
     8101 acacactgtc catcgcgggt catcataaat ccggccatcg atcccagggt ttccgccagc
     8161 gccgccgacg cgccgccatg cagcaggcca aacggctgat gagtacgggt atcaaccggc
     8221 atttcggctt cgagtacatc atcgcccaga cgggtataca caattcccag atgcgccacc
     8281 attgtgttat cgctggtggc gttcagctcg tcgagcgtta aatggcgttt ccagatcatg
     8341 cttatgcccc cagcgtcgag ccgccatcca ccacaatatc ctgtagggtg atatggctgg
     8401 cgaggtcaga ggcgaggaac aaaatcgtgt tggcgatctc ttgtggacgg gcgattttcc
     8461 ccagcggaat gccaagttta aactgctcgc caaagccgcg aatacgctgc tcttcggcgt
     8521 catcgctcac ccacagcgtg cgttgcatat cggtgtcggt ggagcctggc gaaaccacat
     8581 tacagcgcac gccgctaccc gccagttcca gcccaacgct caacgccagg cttttcagcg
     8641 ccgctttcga cgcgccataa gcactcatac caatgcgcgg cgtgtgtgcg gcgtcggacg
     8701 caacagtaac aatcgccccg ccccgctgac ggcgaaactg gttcatggtc tgctggaaca
     8761 ggttaaacgc accgccgaca ttaaccgcaa aagtctgctg ccagtcctcc ttactgagct
     8821 gatcggtcgc gcccatgcgc aaaattcccg ccgcattgac cagcacgtcc agtcgctccg
     8881 tttcagctaa cagtcgctga catacctgcg ccacctgcgc agcgtcggca acatccatca
     8941 cttcggtcgc aaagggatat tgctcctgag tgaacgcttg atcaaaacct gtaactttcg
     9001 ctcccgcctc aacaaacgcc agcgccgtgg cgtagccgat acctttcccc gcgccagtta
     9061 cccagacatt tttaccgctg aaatccatta tttcacctcg cgggagagta gcttccacca
     9121 ggcgtcgatg gtcgggtttt tcgccagcat gacaaagtcg atatcgccat gcactttgcg
     9181 ccagcgcgcc gccagcgcca tcatccgcac cgaatccaga ccgtagtcga tcaggttgtc
     9241 gtcatcgaac ggttcatcgg actcgtccag caacggcagg atcacctcac gcagcgccgc
     9301 tttgctggcg gggataggtg ctggcagtaa ttcttcggtc atcaccaccc ggccagaacg
     9361 tccggccaca tatttcagcg acatcaaatg ctcgtcacgg ctgaaatcgg ccagcgcatc
     9421 tgccaccata aacggtttaa tatcgcgcat aaatgcgtcg gttgcggtgg tcatacagcc
     9481 aatgtgggca tataccccgg taataatcag ctggttacgt ccactctctt tcagcatttg
     9541 ctccagcggg gaacgatgaa acgcgctgta gcgccacttc accagcaccg tgtcgtcggc
     9601 atctggcgtc aggctatcca ccaccttttg ctgttccggc gaacgggtca ggccaggccc
     9661 ccacatatca ttcaacagcg cccgatcttc atcgctctgc tctttcggct gggcggtgta
     9721 ataaaccggg atattgtgct gtttgcagta gtcgcgtagt gccgcaatat tcgcgatcac
     9781 ctgctccatc atcgggcagt tctcgcccca gaagctgaca aaatagtcct gcatatcatg
     9841 gattaacaac gcggcacgtt gcggttcaaa ggcccagtcg actttattct gcggaatatc
     9901 gtgagactcc ggcagtgcgt aagcctgtaa ttttggaata gccatcgtgt tctctccttc
     9961 aggctgatgc gcgtgacgcc agccactgac gtaattgttt tttatcgact ttcccgaccg
    10021 ccgtcagcgg aagtgaatat acacactcca cgcgatccgg taatttaaat tcggcaatac
    10081 cctgttcacg caggaaacga cgcacctgca ccgcgcgcag cggctctttt accaccagat
    10141 aagcacagct tttttcgccc atcagctcat cttccatgct caccagtgcg gcgtagatca
    10201 ccgccggatg gcgcagcagc aggttttcga tctcttcggc agcgatcttc tcgccgccac
    10261 ggttgatctg atctttctcg cgcccctgca cagtgatgta accctctgga tcaatagaga
    10321 tcagatcgcc ggaacagtaa aaaccgttgg catcaaaggc gctggcattg tgctgcgggc
    10381 ttttgtaata gccacggaag gtatacggac cgcgggtcat caggcgtcca acttcccctt
    10441 gcggcagtgg atttccttcg gcatcggcaa cccacacttc atcgtccgga cacattgggt
    10501 aaccctgggt atggataatt ttctccgcgc tatcatcaag tcgggtgtag ttcactagtc
    10561 cttccgccat gccgaatacc tgctgtaact ggcagccaat ctctgcggga atacgcgccg
    10621 caagcgtggc agaaagacgt gcaccaccga cctgtaacag tttcagcgag gcaagctgcg
    10681 cccggctttc gccttcagcc agtgcctgca accacaggct gactgcgggc ggcaccagcg
    10741 cggtaacgtt gacctgatgt ttttcaatca atgggaagca aagcgtggcg ctggggtcgg
    10801 cagccagaac gacagtgccg ccagcgagaa atacccccaa cgatcccggc gaactcatgg
    10861 cgtagttatg agccgccgga atcgcgcaca ggtagcgcgt ctgttgtgtg aactgacaaa
    10921 tctcgacgct acgacgcacg ctgtagtagt agtcgttatg agtgcgcggg atcagtttcg
    10981 gagtgccggt ggtgccgccg gaaagctgga aataggccac ttcatcagca ggagatggcg
    11041 tggcagtaaa atccccagcc ggatggttaa tcgcatcctg caagttatgc tcaccgctgt
    11101 cgttgagcag ctgcaccacg cgaatggaag aatgctctgc aacaaatgtg ttgagaaaat
    11161 catccccgct aaacagcgca tgttggcgat cggcaatcag taacgccggt tcaatctggc
    11221 tggcataggc gttcagttca ctacgctgat ggctgaacag cgccagcacc ggcgcaacgc
    11281 ccagtttcag cagcgcgaaa aaggtaatgt aaagttcagc gacgttaccc agttgtacca
    11341 gcgcggtttc accaggttta atgccctgac ggcgtaaact acacgcgagg ttatccgccg
    11401 cctgattcag ctcccggtaa ctcaactgcc gctcgccgtc gataaccgcg atgctgtcac
    11461 tcgcagcatg tcgcgtcaga atgtcggtca gcggcaaatc ctgccagtag cctttttccc
    11521 gatagcgacg ggcaaactct tccggccagc gggtaaatgg aatgctcatc ctcgctcctt
    11581 aatgcaatcc aaaaacgttc aacatggtag aaagtttgac gcctgtttcg cgccactcac
    11641 ccaacggtga cgacgcaggc acaatccccg ctccggcaaa cagacgcacc tgattttccc
    11701 gcagcttcgc gcagcggatg gtcaccaccc actcgccgtt accttcgctg tcacaccaac
    11761 ccacaatgcc gccaaacagt tcgcggtcga acggctccag ttcagcaata acctgggtcg
    11821 cggcctgatg cgggaaaccg ctcagcgcgg gggtcggatg cagcagacag gccagcgtca
    11881 gtgcgttctc atgcgaattc gctttacctt caaagggagt tgcgagatgc cacagcgtcg
    11941 gcgtggtgat cagttgtgga gaagaaggga cgtgtaactc actacttcgt tcgcgcagta
    12001 cctctttcat cgcctgagtc accagttcat gttcatggcg atctttttct gacgccagca
    12061 gacgattacc cgcttcgcga tcgagcactt catccggctg acgacgcgcg gaaccggcta
    12121 acggaatgga gctaaaacgc tcgccgtctt tacgtagcag cagttccggg ctggccccca
    12181 gcagaacgcc accatcagcc agcgggacat ggaagttgta actaaccggg ttttgcgcaa
    12241 tcaaccgttc cagcaatacg ccactatcaa tggcggcgtc agtggtgata tcaatcaacc
    12301 gtgacaacac cactttgtcg acctgcggcg tggcggtaag tgcggcggcg cgagcaacca
    12361 tctgttcaaa cgtggcttgt tccggaattg cctggcgttc caccacgttc agcgactggc
    12421 tgcgggtaaa acggcgggct gaagattgtt tttcctggcg ggagaacgac tgccaggact
    12481 cgggaatata cagcgatgaa ggctgacgtg gatcgaaggg aatcgccccg accatcaccg
    12541 gatttttgat gccctgcgct ttggcatcgg caaacagcgc ggcgagtttt tgctggaagg
    12601 gactgtcggg tgaatccccg ttcacagccg gttcatcgaa gcgggcgaaa catcctgacg
    12661 tcgtaaaact gcggtacggc gacataaaga aaaagcgatt gggcgcaagt gttgccatgg
    12721 tctgctgtac ttcctcagcc agtgacgtat ccatatcatc ctccacaaaa tgataaaggc
    12781 tttaataatg attatcattt atattttcgg tcgctaacct aaaagcaaac gcgcactatg
    12841 tcaactcttg aggtaacgca atctgcaact cggggttgca tctggttgtg gttacaatga
    12901 aaatgagaag cattaacttc attaataaca ggaagctgac ttgtgagact cgccccgctc
    12961 taccgcaacg cccttctatt aacaggactt ttgctttcag gaatagccgc agttcaggct
    13021 gccgactggc cgcgtcagat tactgacagc cggggcactc ataccctgga aagccagccg
    13081 cagcgtattg tttccaccag cgtcaccctg accggctcac tgctggcgat tgatgctccg
    13141 gtgatcgcca gcggcgcgac cacgccgaat aaccgcgtag cggatgacca gggcttttta
    13201 cgccagtgga gcaaggtggc gaaagaacgc aaactgcaac ggctctatat cggcgaaccg
    13261 agcgccgaag ccgttgccgc gcaaatgccg gatctgattt taattagcgc aaccggcggt
    13321 gattcggcgc tggcgctgta tgatcagctt tccaccatcg ccccgacatt aatcatcaat
    13381 tacgacgaca aaagctggca ggcgctgtta acgcaacttg gcgaaattac cgggcatgag
    13441 aaacaagcgg cagagcggat tgcgcagttt gataagcaac tggcggcggc gaaagagcaa
    13501 atcaaattac cgccgcagcc ggtcactgcc attgtctata ctgccgctgc acacagtgcc
    13561 aatctctgga cgccggaatc agcacaaggg cagatgctgg aacaactcgg ctttacgctg
    13621 gcgaagttac ccgcagggtt aaatgccagc caaagccagg gtaaacgcca tgacatcatt
    13681 cagcttggtg gggaaaatct ggctgcaggg ttaaatggcg agtcactatt cctgttcgcc
    13741 ggtgatcaga aagatgccga tgcgatttat gccaatccgc tgctcgcgca cctgcctgca
    13801 gtacaaaaca agcaggttta tgcgctggga accgagacgt tccgactgga ttactacagc
    13861 gccatgcaag tgctggaacg acttaaggcg ctgttttaag cattaactgt cggacgctgt
    13921 cacctgcggc ggcgtctggc gaaaacgccg caactccacc agcaccagca ataacaacac
    13981 gccgataatc aacaaaccaa aaccgctcgc gcttgcggaa gcaaccggtg tcatcatcgc
    14041 tcccagaccg cctaacagcg ccgcgcctat ggcatcgccc gtcacgtttt gcgccgtcca
    14101 caaaccgtta atccgcccta acatcgcttc cggggtttgc gtttgcagca ttgtgtattg
    14161 cagcaacgag ctgaccgcac tcaaccagcc gaacagcgcc agacaaacca cgcctaaaat
    14221 ccacatcggc atcaggccaa acagaccaat ggcgaggaac gatcccagcg tggagagcag
    14281 catcaataac cctggtcgcg cactatgtgc cagcttcccg ctggttaacg caccaatagc
    14341 cgcgccgagc gggatcgccg catagagaaa accaatctgt gccgccgaca tctgccagtt
    14401 gtcagccagc gccggataca gtacccgcac cgcgctcgcc atcgtcaata aaccacccaa
    14461 cagcgcaatc cctcctacca gcgggctggc gagcagaaaa cgaaatcctg ccagtaatga
    14521 tttcaacgga tgctcacgcg gctgcggtgg tggtggtaac gccggaaggc ttaacaacgg
    14581 tagcaaggta ataaacgtgc ccgccgccgc cagcccgtag ttccaggcta cgcctccggt
    14641 cgccagcagt aaaccgccaa tcatgggcga aatcaccgat cccaggcgca cagtcaacat
    14701 ggtgatcgcc ccggcctgca ttaagttttc acgccctacc agtgcaggtg tcgccgccag
    14761 tagcgccgta acgccaagtg atgcgaaaaa accatcccat aaaccaagta aatagattgc
    14821 cagcaatgac ggctccggca gcagtgcatt aaggcacagt ccaatgaagc caatgccaca
    14881 ggtgccgcgc gccagcaaaa tcactttttt gcgctcatag cgatccgcca gcacaccgcc
    14941 gaccatcagg ccaacaaaca tcgcgccgcc ggtcagcgtc accgaaagcc ccacctgcca
    15001 ggtagaatgc gtcattatct ggatctgcac cggtaccgcg acgccgagca aacccagaga
    15061 cacaattgag ataaaacgag cgaggaatac tgcgcgaaac gccgggtgcg ttttcaacag
    15121 gctgaggtta agcagccagg attgtttatt cattacaatg ccttgccatc aaatatttta
    15181 aatacctatt cttaggctgc acatgctaac atatccaaat aagatcgata acgataatta
    15241 atttcattat catggaagtt cgtatgtctg gttctgttgc cgtgacacgc gccattgccg
    15301 tgcccggatt gctgttatta ctgattatcg cgacggcatt aagcctgctc attggggcaa
    15361 aatcactccc cgcttccgta gtgctggagg ccctctccgg cacctgccag agcgccgact
    15421 gcaccatcgt gctcgacgcg cggctgccgc gcacccttgc cggtttactg gcaggcggcg
    15481 cgcttgggct tgccggggcg ttaatgcaaa ccctcacccg aaacccactt gccgaccccg
    15541 gcttgcttgg cgtaaacgcc ggagccagct ttgccattgt gctgggtgcg gcgctgtttg
    15601 gttactcatc cgcgcaggaa caactggcga tggccttcgc cggggcgctg gtggcctcgt
    15661 tgattgttgc ctttaccggc agccagggcg gcgggcagtt aagtccggtg cgtttaaccc
    15721 tggcgggcgt ggcgctggcg gcggtgctgg aagggctgac cagcggcatc gccctgctta
    15781 atcctgacgt ctacgatcaa ctgcgtttct ggcaagccgg ttcgctggat attcgcaatc
    15841 tatatacctt aaaagttgtg ctgatcccgg tgctgatcgc cggagcaacc gcgctattac
    15901 tgagccgcgc gctgaacagt ttaagcctcg gcagtgacac cgcgacagcg ctgggcagcc
    15961 gcgtggcgcg cacacagttg attggtctgc tggcgattac cgtgctttgt ggtagtgcga
    16021 cggcggtagt tggcccgatt gcctttattg gcctgatgat gccgcatatg gcgcgttggt
    16081 tggttggagc agatcatcgc tggtcgctgc ccgtcacgct acttgccacc cctgccctgc
    16141 tgctgtttgc cgatatcatc gggcgggtga ttgtgcccgg cgaactgcga gtttccgtgg
    16201 tcagtgcgtt tattggtgca ccggtgctga tcttcctcgt gcgacgtaaa acgcgaggtg
    16261 gcgcatgatt tacgtctctc gccgattaat tatcacctgt ttgctgctgg tttccgcctg
    16321 tgtggttgca ggtatctggg gattacgcag cggtgccatc acgttggaaa cctcgcaggt
    16381 attcgccgcg ctgatgggcg atgcgccgcg cagtatgacg atggtggtca ccgaatggcg
    16441 tttaccacgc gtgctgatgg cgctgttgat tggcgcggcg ctgggcgtca gcggcgcgat
    16501 ttttcagtcg ctgatgcgta acccgctcgg cagccctgac gtaatgggct ttaacaccgg
    16561 ggcatggagc ggcgtactgg tggcgatggt gctgtttggt caggatctga cggctatcgc
    16621 gctggcagca atggcggggg gtattgtcac ctcgctgttg gtctggctgc tcgcctggcg
    16681 taacggtatc gacacctttc ggttgattat tatcggtatc ggtgttcgcg ccatgctggt
    16741 ggcctttaat acctggctgt tgctgaaagc gtctttagaa acggcgctaa cagcgggttt
    16801 gtggaatgcc ggatcgctca acggcctgac gtgggcaaaa acctcacctt ccgcgccaat
    16861 cattatattg atgctcattg ccgcctcctt gctggtgcgg cgaatgcgct tgctggaaat
    16921 gggcgatgat accgcatgtg cgctgggcgt cagcgtcgaa cgttcgcggc tgttaatgat
    16981 gctggtcgcc gtagtgctta ccgctgcggc aacagcgctt gccgggccga tttcctttat
    17041 tgctttagtc gcgccgcaca ttgcccgccg cattagtggc accgctcgct gggggctaac
    17101 ccaggcggcg ctgtgcgggg cgttgttact gctggtggcc gatctctgcg cccaactact
    17161 gtttatgccg tatcaacttc cggttggcgt cgttaccgtc agcctcggtg gtatttacct
    17221 tatcgtcttg ttaattcagg agtctcgcaa aaaatgaccg aatcagtagc ccgtttgcgc
    17281 ggcgaacagt taaccctcgg atatggcaaa tataccgttg cggaaaatct gactgtagaa
    17341 atacctgatg gtcacttcac ggcaattatc gggccaaatg gctgcggtaa atccacgttg
    17401 ctgcgtacct taagccgcct gatgacgcct gctcacgggc atgtctggct ggatggcgag
    17461 cacattcaac attacgccag taaagaggtt gcacgccgga ttggcctgtt ggcgcaaaac
    17521 gccaccacgc cgggagatat caccgtgcag gagctggtgg cgcgtgggcg ttacccgcat
    17581 caaccgctgt ttacccgctg gcgcaaagaa gatgaagacg cggtaacgaa agcgatgcag
    17641 gccacgggaa tcactcatct ggcagatcaa agcgtggata ccctttctgg cggacaacgc
    17701 cagcgagcgt ggatagcaat ggtgctggcc caggaaacgg caattatgct gctcgatgaa
    17761 cccacgacct ggctggatat cagtcatcag attgatttgc tggaactgtt aagcgaactg
    17821 aaccgcgaga aaggctatac cctggcggcg gtgctgcacg atcttaatca ggcctgtcgt
    17881 tacgccagcc atttgattgc attgcgggaa gggaaaattg ttgctcaggg agcgccgaag
    17941 gagattgtca ctgctgaact gctcgagcgc atttatggtc tgcgctgcat gattattgac
    18001 gatccggttg ccggaacgcc gctcgtggtg ccgctcggac gaacgacacc ttcaaccgcg
    18061 aagatttaaa ctaagtggtc tgccattatg gcatcctgtt ttctggatgc catcgcatgg
    18121 cgcaatagca cgtcaccaca agccactata ccgccgatca acgcagaaag gatcacaata
    18181 atcgccttac ccggaccgtc tttttttacg ggtaatgacg gacgcaactg atatttaaac
    18241 ggtgtaaaat tcacatcgtt gacatttgtt tttgttaatt gctcgactag atactgacga
    18301 ttacgtaatt caccgttcaa ttccgcaacg tcagtgactg ctttttcaat ttccagtttg
    18361 cgttcaatac cgtctgcgcc gagagaaata gagaaatcgg ggtcatcttt aacggcctga
    18421 ccattactgt atacgggctt tttaattcct gccgcgttgg caatgtcgag tgaataattg
    18481 aggcgctgaa tgtttgcatc aagttgattt ttcgttttaa tgcgatcctg agccagtttt
    18541 tctttttcaa actgggtttt gatctccagt ttattacgga cgttttctat cgactctttc
    18601 accaccaacg cagagatata atcgatatac ccggccaaca ctttctgtgc ctcttcactg
    18661 gtgggtgcgg tgaaacttag cgtccaggaa gtatacaatg cagactcatc ttttttctta
    18721 ctggcattgt catcaaccgc tttcattttt tcgctcaatg cgacaattgc gcgatgcaaa
    18781 tccagttcgt cgattttcgc ctcttttaat tggtccatca cataaggtgt tgagcgcagg
    18841 tactcttcca gcaagctaac cgactgaaac ttcttgataa acaggttaaa tgcttctgta
    18901 cgatcaattt tgatatccag atccagcaca cgaagcttag tgaatgtttt ctcaagcgcc
    18961 tgccactgaa caggttctgg aggcgtgaca accgccgcgc tggtccattt ttgcggcagg
    19021 atgaaagaga tcagtaaacc tgcgcaggca aacgcaaaaa cgaccgccat gaccgttttt
    19081 ttggcccgcc ataaaacttc aattagatta agtaaatcaa cttcattatt actgggcgac
    19141 gccagaggat actcgggaaa atgagcgtca cttccctgtt taatattcag tgatgacatg
    19201 gggtataacc tgaaaatgag tccaataggg ggaaatctcc acggcgctca ctttaccagt
    19261 tggtgagaat tatccgaagc tgaagtttgt aaattccttc cactgaatgc aacattcact
    19321 ctatttaaga gaaatatcca ccaaacttat aaattaaata aatgataaac ccataatcat
    19381 ttcctgtaat tatgggttta tggataatat taatttacct gtttagcgta gcgcgaataa
    19441 tcggcccaat tttttcaaac gcccctggag agataatatc cacatgcgca caatcctgac
    19501 gatagatatc cagctccgca atccacggcg accaggcgcg ttcgggattc ataccttcct
    19561 gaagtgtgcg ttcagcaaca aacagcgtcg ctttaccttc aaacggtacg ctatgagcag
    19621 tcgtcagcag gcgcacagca tcagcgtagt tgccttcaat ggtggtaaac aaatccgttg
    19681 aagtacttcc ctgttgtgcc gccaggaagg cctcgcgctc gcggttaatc tccgccagca
    19741 cttccgggtc caggccatta gcctcttttt cctgccagtt ttgcgtttct ggcggccagg
    19801 tatccagcaa gccaagaaat gccacctgtt cgccacgggc acgcagtcgc gccgcaatac
    19861 cctgcgccag cgtaccgccc agcgaataac ccagcaggta ataagggccg tgcggttgat
    19921 gctcaagtaa cgttgccaga tgcgcttcgc agacttcatc cagatttgcc gcggtctgca
    19981 tgggaccatt ggggcgcggc gactgaatgc cgataatcga ccattgtgga tcgagataac
    20041 gcgagagcac gctgaactgc caggcaaaac cggacgcagg atggaaacaa aacagcgtcg
    20101 ggccattacc ttcacgcaac ggcagaatgg tttcgaatcc catgcgccgg gagctgtcct
    20161 cgtcaccatc aataatcgtt gccagtttgg cgacggttga tgcgaccatc acctgccccg
    20221 gcgtcacctg acgggcaaac tgccgactta actgcgcagc cagtttcatt gccagtagcg
    20281 aatgaccgcc aagcgcaaag aaatcagcat cggcatcctg cacgtcacaa cccagcaacg
    20341 acgagaatgc cgcggcgata atcgtttcac tgcccgcttt cggcgcacgc cccggcgctt
    20401 gtgccttcag ttcaggcaac ggtaaggctt tgcgatccag cttgccgttg gcgctaagcg
    20461 gcaactgcgg aagttgcagc agaaccacgg gcaccatatg cggtggcaat gtttcgcgca
    20521 actgcgcctg caatgcagtg gtatccagcg gcaggcctga ttgcgacacc agatacccaa
    20581 ccaactgacg cgcatcgcct cccgttgcgg cagcctggtt aatgacgcag gcgtgggtca
    20641 cggcctgttc gacatccggc agtgcctgca tcacgcgatc gatttcgccc agttcgatac
    20701 gctgcccgcg aatttttagc tgatcgtcac tgcgcccgag gtactccacc gcgccgttat
    20761 ccagccagcg ggcaacgtct ccggtacggt acatccgttc gccaggggca aaaggatcgg
    20821 caataaagcg gctggcggtc agatcgggtc gtccaagata cccctgcgcc agttgaatac
    20881 cggtgagata gagatctccc gccacacccg gcggcaccgg atgcatcatc gcatcaagaa
    20941 tacgtaggcc tgtattccac accggataac caatcggcac actgctgccg cgcacctgtg
    21001 ccagttcctc gccaaaagcc ggataccagc tgacatctac cgccgcttcc gtcgggccat
    21061 atagattatg caacggcacg ctggttaact gttgccattc gcggcataaa tcagcgggta
    21121 aggcctcacc actacagaaa acctgtttca acgtcgcgca attctgacga gcggtttgcg
    21181 gagtcagcga tgcaacaaat gctgccagca tcgacggcac aaagtgcgtg gtcgttacac
    21241 catattcggc aaagaattgc tgcatagcga gcgggtcgcg gtgcgcttcc ggttcagcca
    21301 tcaccagttt tgcccctgcg ataaacggcc agaaaaactc ccacaccgag acatcaaaac
    21361 tgcacggcgt tttttgggca acgacatctt cgcctgtaag cgggtagtgg ttttgcatcc
    21421 aaagcaggcg gttaacgata gccgtctgcc cgaccattac ccctttcggt ctgccggtgg
    21481 agccagaggt aaagatgata taagccgtgt ggtgcggttg tgaaagtttc agcggcgcac
    21541 tgccctgcgg tgtaagcggg gcgttatagc aaaggctggt taaatctgga acatcggcaa
    21601 agcgcggcag ttgatcgtcg gtggtgatta acagcgacgg acgcgcatct tccagcatca
    21661 ttttcaggcg ttcgtccgga tagccggtat ccagcggtaa ccaggccgca ccagcctcaa
    21721 caattgcatg tagcgccaga gtcaaaaaga ccgagcgcgg tagcgccacc gccacgctgt
    21781 cgcctggttt aacgccgcgc tcacgcagca gattcgccag cgccaccacc tgctcgcgca
    21841 tttcccgata gctgaacagg taacgcgcat ctgccagcgc cggagcatgc ggtgtttttg
    21901 ccgcttgttc tgccaccagc gcgctcagcg tggtttctgg aatctcaacc tgagtggcgt
    21961 tgatctgcgc cagctgtgca tactcttctg gcagcataat atcaacatcg ccgcacaaca
    22021 gcaccggatc cgcggcgaac tgggcaatca gcattttcag gcgttcagca tgctggatta
    22081 acgttggctc agcgtaacgc tgtttattgg cgatgatctc aatactcaaa tcaccgtgtt
    22141 catccgggaa cagggccagc tcaaggtcat taaccggacc ggttgccagg gtatgggttt
    22201 gcgcctgaac atcaggaata tccagttggt aatcaaatac cttgatattg agtaccggac
    22261 caaacagcgg ttcatcacct gccgctcgcc cactgtcacg gacaatttgt tcggcgtcgt
    22321 agcgttgatg gcggcgcatt tttttcagtt gtgctgccag tcgggttgcc agttgcggca
    22381 gcgtttcttg cgccgcaatg tgaataccca acggtaaaac gttgagcacg ggtccggtag
    22441 ccgtcagcgc cgccgagccc agtcgacgca taaagataaa tccggcagcg tagtccatgc
    22501 ggttgcacaa tcgccccagc cacaaagccg tcagcgcaag ggctaaatcg gtacgctgca
    22561 cacctgaaag ttgcgtagcc agctggcgga attccccgtc ggtacattcc agtttcaggc
    22621 gcaaaatatc tgccgaagcg ctgcgccccg gtaaaggtgc cggagaaagt gacgcgggcg
    22681 gtggcagttg acgacgctgt tccgcccaga atgccgcatc acgctgccag gcttcgcttt
    22741 cgcggtattg ctggtactct tccactacat cagcgaaagg cgtaaacggc gaagcaggcg
    22801 ttggttcgcc acgcagccat gcgcagtaaa tattggcgat ctggcgggta atggccggga
    22861 aactgaagcc atcgaccagc aaatggtgat aacgctgata ccagtaccag cggttatccg
    22921 ccacctgtat caactgatga aagaccagtg gtttaccgct atcgacgcgc agatcttgtt
    22981 gcaaatccgc ctgcattaat gactgcgcag taccgtgcgg atcaatgttg gttcgcaggt
    23041 cgatagtttc tggcagttcg aacgtcagcg catcatcgac ccactgccag acttcgccgt
    23101 tatcttccgt aaaacgcatc cgcagcgtat ctgcttgcgc taatccggca accaccgcgc
    23161 gggccagtaa tggcgcatca acctctccgg ttaactcaac gtaatgcgcc acgctccagg
    23221 cggagggtaa atccgacagt ttttctgcca tccagatgcc gggctgtgcg gcgaccaaag
    23281 gtaaatgctg gctcattgtg cctcctgcaa ctgggtgaaa ttcgccggtg tcagagtacg
    23341 ccagtgggct tccagccact gctggcagga cgcctgtgac tgcggctgac acacaacgtc
    23401 ccagcctgcc ggtaaggcgc attgttgcgg ccacagactg aattgcccct gcgcattgcg
    23461 caaaatgtaa aacgctccct gcggatcatc gaagggatta ctgaatgcca tattcaactc
    23521 ctgtcatgga aaagtggttg ccagaggtcg attagcccct gcatcaagcc accgcgccaa
    23581 caaagcgcat catgtccgcc gtcaacctga cgccagaaaa tggattcttt tatggggtgt
    23641 aattgcgcat acagcgcctg attggcctgc atgatcatcg gctcgcgaat acccgcttcc
    23701 agcacaatgc gcagaccttc ggcactaact tgaccagctt taagttgctc aagtaaaaca
    23761 ccttcttgct gcccgccccg atgcggccac cagtacgatc ctgactggct taatacacag
    23821 ccaaagcgtt caggccagtg cagtccggca tacagcgcgg aaagcccacc aaaactctgc
    23881 ccggcgacca cggtgcgatc ggcgcgatcg ctaaaagggg caatagcttt caccaggggt
    23941 aataactctt gctgtactgc cagccagaaa tccgcattac acggcagttc gtgggcgcgg
    24001 tgcgtggtgt caatagcgtc gatcaacaca tacacggctg gaggaagttg ctgacgatgg
    24061 gtcaacgaag tcagcacagg ccagacaggc atactctgcg cccaaaattc gccatcgagc
    24121 aaaactgcca gcggacgttc tcctgctgtt gcatcgccag tggtaaaaat ccatacacgg
    24181 cgtgaatttt tcaaccgctc acttttccag ataatttctt tggcaggtgt ttctggcgct
    24241 tgcggacaat cccatcccgg ttgtaggggc gcttgcggca tttcgagtgc agaaacagcg
    24301 tgccctcgcc cgcctttcca gctttgtgga ttcagcggat cggctatcgc ctggggtaat
    24361 agttttcgcc agccttcacg taattcgagg cgatcggggg atggtgcaga aaaaatgtca
    24421 tcgcgttcgg tgggaataaa acagtagctg ccgcgccagt tggcattaag ttgtgtcgtc
    24481 cactgccaga cgttggtgcc tgcaattcgc tgcatcgact ggggctggct gttctgatgg
    24541 tgatcggtca caccagtgat gtagacccat acgcgcttta tcgtcgagta ttcttcagaa
    24601 ccctggggat cacgccaccg gaaagtgacc tcaaacattt cgtcattcag acgctgccat
    24661 tccgggccat gtttcgactg ccaccagctc tcacttccta cttttaacgc cgtcacacca
    24721 taaccccatg tttactgtgc aatttttcat tgattgcaga aatatattga taatattatt
    24781 gataactatt tgcatttgca atagcgtaat ggcgcgccgt gggaagcgcg gacattaatt
    24841 aaccaactgc actgcgtgtc tttcaggatc aaaggttttc gcggtagcgg gatgcgtcgt
    24901 gttgatgacg accatgcccg acagttgcaa ttcgtggcaa aaatgcagga ataaaacaat
    24961 gaacaagaag attcattccc tggccttgtt ggtcaatctg gggatttatg gggtagcgca
    25021 ggcacaagag ccgaccgata ctcctgtttc acatgacgat accattgtcg ttaccgccgc
    25081 cgagcagaac ttgcaggcac ctggcgtttc gaccatcacc gcagatgaaa tccgcaaaaa
    25141 cccggttgcc cgcgatgtgt cggagatcat ccgtaccatg ccaggcgtta acctgaccgg
    25201 taactccacc agtggtcagc gtgggaataa ccgccagatt gatattcgcg gtatgggtcc
    25261 ggaaaacacg ctgattttga ttgacggcaa gccggtaagc agccgtaact cggtgcgtca
    25321 gggctggcgt ggcgagcgcg atacccgtgg tgatacctcc tgggtgccac ctgaaatgat
    25381 tgaacgtatt gaagttctgc gtggtccggc agctgcgcgt tatggcaacg gcgcggcggg
    25441 cggcgtggtt aacatcatta ccaaaaaagg cagtggtgag tggcacggct cgtgggacgc
    25501 ttatttcaat gcgccagaac ataaagagga aggtgccacc aaacgcacta actttagcct
    25561 caccggtccg ctgggcgacg aattcagctt ccgcttgtat ggcaacctcg acaaaactca
    25621 ggctgacgcg tgggatatca accagggcca tcagtccgcg cgtgccggaa cgtatgccac
    25681 gacgttacca gccgggcgcg aaggggtgat caataaagat attaacggcg tgggtgcgct
    25741 gggacttcgc accattgcaa tcgctggaac tggaagcggg ttacagccgc cagggtaacc
    25801 tgtatgcggg cgatacccag aacaccaact ctgactccta tacccgctcg aaatatggcg
    25861 atgaaaccaa ccgtctgtat cgccagaact actcgctgac atggaacggc ggctgggata
    25921 acggcgtgac caccagcaac tgggtgcagt acgaacacac ccgtaactcg cgtattccgg
    25981 aaggtctggc gggcggtacc gaagggaaat ttaacgaaaa agcgacacag gatttcgtcg
    26041 atatcgatct cgatgacgtg atgctgcaca gcgaagttaa cctgccgatt gatttcctcg
    26101 ttaaccagac gctgacgctg ggtacggagt ggaatcagca gcggatgaag gacttgagtt
    26161 ccaacaccca ggcgctcacc gggacgaata ccggcggcgc tattgatggc gtgagtgcca
    26221 ccgaccgtag cccgtattca aaagcagaaa ttttctcgct gtttgccgaa aacaatatgg
    26281 agctaactga cagcaccatc gtaacgccgg ggctgcgttt cgatcatcac agtattgtcg
    26341 gcaataactg gagtccggcg ctgaacatat cgcaaggttt aggcgatgac ttcacgctga
    26401 aaatgggcat tgcccgtgct tataaagcgc cgagtctgta ccagactaac ccaaactaca
    26461 ttctctacag taaaggtcag ggctgctatg ccagcgcggg cggctgctat ctgcaaggta
    26521 atgatgacct gaaagcggaa accagtatta acaaagagat tggcctggag ttcaagcgcg
    26581 acggctggct ggcgggcgtc acctggttcc gtaacgatta tcgcaataag attgaagcgg
    26641 gctatgtggc tgtagggcaa aacgcagtcg gcaccgatct ctatcagtgg gataacgtac
    26701 cgaaagcggt ggttgaaggt ctggaaggat cgttaaacgt accggttagc gaaacggtga
    26761 tgtggaccaa taacatcact tatatgctga agagtgaaaa caaaaccacg ggcgaccgtt
    26821 tgtcgatcat cccggagtat acgttgaact caacgctgag ctggcaggca cgggaagatt
    26881 tgtcgatgca aacaaccttc acctggtacg gcaagcagca gccgaagaag tacaactata
    26941 aaggtcagcc agcggttgga ccggaaacca aagaaatcag tccttacagc attgtgggtc
    27001 tgagcgcgac ctgggatgtg acgaagaatg tcagtctgac cggcggcgtg gacaacctgt
    27061 tcgacaaacg tttgtggcgt gcgggtaatg cccagaccac gggcgatctg gcaggagcca
    27121 actatatcgc cggtgccggg gcgtatacct ataacgagcc gggacgtacg tggtatatga
    27181 gcattaatac tcacttctga tgctaacgtc agattgttga caaagtgcgc gtaattcatg
    27241 ccggatgcgg cgtgaacgcc ttatccggcc tacaaaaatc ttgccaattc aatatattgc
    27301 aggaccacgt aggcctgata agcgtagcgc atcaggcagt tttgcgtttg tcatcattct
    27361 ctaatatggt cgacatgaaa actacgcata cctccctccc ctttgtagga catacgctgc
    27421 attttgttga gtttgatccg gcgagttttc gcgagcagga tttactctgg ctgccgcact
    27481 acgcgcaact gcaacacgct ggacgtaaac gtaaaacaga gcatttagcc ggacggatcg
    27541 ctgcagttta tgcgctgcgg gaatatggct ataaatgtgt gcccgcaatc ggcgagctac
    27601 gccaacctgt gtggcctgcg gaggtatacg gcagtattag ccactgtggg gctacggcat
    27661 tagccgtggt atctcgtcaa ccgattggca ttgatatcga agagattttc tctgtacaaa
    27721 ccgcaagaga attgacagac aacattatta caccagcaga acacgagcga ctcgcagaat
    27781 gcggtttaac cttttctctg gcgctgacac tggcattttc cgccaaagag agcgcattta
    27841 aggcaagcaa gatacaggcc gctcaaggtt ttctggatta tcaaataatt agctggaata
    27901 aacagcagct catcattcga ctagaggatg agcagtttgc tgtgcactgg cagataaaag
    27961 aaaaaatagt cataacgctg tgccaacacg attaattgac aacatctggt acgattcgcc
    28021 cgcagccatc actgaccacg ggcgaaagtg taaagcaggt gccttacca
//
LOCUS       DAPHCV010000065        27827 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.065, whole genome shotgun sequence.
ACCESSION   DAPHCV010000065 DAPHCV010000000
VERSION     DAPHCV010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.065"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            100..363
                     /gene="yhcN"
                     /locus_tag="RH033_002737"
     CDS             100..363
                     /gene="yhcN"
                     /locus_tag="RH033_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HDV2991901.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(418..690)
                     /locus_tag="RH033_002738"
     CDS             complement(418..690)
                     /locus_tag="RH033_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HDV2991902.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(782..2749)
                     /gene="aaeB"
                     /locus_tag="RH033_002739"
     CDS             complement(782..2749)
                     /gene="aaeB"
                     /locus_tag="RH033_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HDV2991903.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            complement(2755..3687)
                     /gene="aaeA"
                     /locus_tag="RH033_002740"
     CDS             complement(2755..3687)
                     /gene="aaeA"
                     /locus_tag="RH033_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HDV2991904.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYIGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            complement(3695..3898)
                     /locus_tag="RH033_002741"
     CDS             complement(3695..3898)
                     /locus_tag="RH033_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HDV2991905.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            4081..5010
                     /gene="aaeR"
                     /locus_tag="RH033_002742"
     CDS             4081..5010
                     /gene="aaeR"
                     /locus_tag="RH033_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HDV2991906.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(5138..6583)
                     /gene="tldD"
                     /locus_tag="RH033_002743"
     CDS             complement(5138..6583)
                     /gene="tldD"
                     /locus_tag="RH033_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HDV2991907.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(6739..10539)
                     /gene="yhdP"
                     /locus_tag="RH033_002744"
     CDS             complement(6739..10539)
                     /gene="yhdP"
                     /locus_tag="RH033_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HDV2991908.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGDV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLPVNVKVYGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTH
                     GDSTNSSPFPTTERISFRGWPDAQIRCAECWFWGQKFGRIDSDITISGNTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(10607..12076)
                     /gene="rng"
                     /locus_tag="RH033_002745"
     CDS             complement(10607..12076)
                     /gene="rng"
                     /locus_tag="RH033_002745"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HDV2991909.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(12066..12659)
                     /gene="yhdE"
                     /locus_tag="RH033_002746"
     CDS             complement(12066..12659)
                     /gene="yhdE"
                     /locus_tag="RH033_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HDV2991910.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(12668..13156)
                     /gene="mreD"
                     /locus_tag="RH033_002747"
     CDS             complement(12668..13156)
                     /gene="mreD"
                     /locus_tag="RH033_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDV2991911.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(13156..14259)
                     /gene="mreC"
                     /locus_tag="RH033_002748"
     CDS             complement(13156..14259)
                     /gene="mreC"
                     /locus_tag="RH033_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDV2991912.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGITQPTPQQPATGNAVTA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(14325..15368)
                     /gene="mreB"
                     /locus_tag="RH033_002749"
     CDS             complement(14325..15368)
                     /gene="mreB"
                     /locus_tag="RH033_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HDV2991913.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(15673..17613)
                     /gene="csrD"
                     /locus_tag="RH033_002750"
     CDS             complement(15673..17613)
                     /gene="csrD"
                     /locus_tag="RH033_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HDV2991914.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTND
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLCEIQNAREQHSRLDT
                     LIRSYAAQDMKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFSLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGRVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRRHIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            17765..18739
                     /locus_tag="RH033_002751"
     CDS             17765..18739
                     /locus_tag="RH033_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDV2991915.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEVVVTGASGGVG
                     STAVTLLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPARRA
                     QAWQRLVADLPESFYTQAAKEISLAEAPKFAEAIINNQIQGRTLVKVN"
     gene            19717..20187
                     /gene="accB"
                     /locus_tag="RH033_002752"
                     /gene_synonym="fabE"
     CDS             19717..20187
                     /gene="accB"
                     /locus_tag="RH033_002752"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDV2991916.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            20198..21547
                     /gene="accC"
                     /locus_tag="RH033_002753"
     CDS             20198..21547
                     /gene="accC"
                     /locus_tag="RH033_002753"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDV2991917.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            21656..21898
                     /locus_tag="RH033_002754"
     CDS             21656..21898
                     /locus_tag="RH033_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HDV2991918.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAHLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            21888..23339
                     /gene="panF"
                     /locus_tag="RH033_002755"
     CDS             21888..23339
                     /gene="panF"
                     /locus_tag="RH033_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HDV2991919.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVDTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            23351..24232
                     /gene="prmA"
                     /locus_tag="RH033_002756"
     CDS             23351..24232
                     /gene="prmA"
                     /locus_tag="RH033_002756"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDV2991920.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVAKEEWCRITGRKN"
     gene            24561..25526
                     /gene="dusB"
                     /locus_tag="RH033_002757"
     CDS             24561..25526
                     /gene="dusB"
                     /locus_tag="RH033_002757"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDV2991921.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            25552..25848
                     /gene="fis"
                     /locus_tag="RH033_002758"
     CDS             25552..25848
                     /gene="fis"
                     /locus_tag="RH033_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HDV2991922.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            25934..26818
                     /gene="yhdJ"
                     /locus_tag="RH033_002759"
     CDS             25934..26818
                     /gene="yhdJ"
                     /locus_tag="RH033_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HDV2991923.1"
                     /translation="MRTGCEPTRFGNEAKTIIQGDALTELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHHVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            26902..27081
                     /locus_tag="RH033_002760"
     CDS             26902..27081
                     /locus_tag="RH033_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HDV2991924.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFHDRM"
     gene            complement(27084..27746)
                     /gene="acrS"
                     /locus_tag="RH033_002761"
     CDS             complement(27084..27746)
                     /gene="acrS"
                     /locus_tag="RH033_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HDV2991925.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQDHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCIANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VI"
BASE COUNT         6797 a         7382 c         6958 g         6690 t
ORIGIN      
        1 gcgtggtatt aattagttca ataattagtg tatacttgat tttgtgatat gggtcacgaa
       61 acaaaggccc agctaaaaga ttatgtcgag gtaaaaatca tgaaaatcaa aaccactgtt
      121 gctgcattaa gcgtactttc tgttctctct ttcggtgcat tcgctgccga ctccattgat
      181 gctgcacaag cacaaaatcg tgaagcaatc gggaccgtat ccgtaagtgg tgtggcgtct
      241 tcgccaatgg atatgcgtga aatgctgaac aaaaaagcgg aagagaaagg cgcaacggcc
      301 taccagatta ctgaagctcg tagtggtgac acctggcacg ctacggctga actgtacaaa
      361 taaaccctca tcgtcttgtc cgacgatatt gcccccggtt cgggggcttt ttttgcgcta
      421 atgacgaaca ttaaaacgca aatgcccttc cagctcttcc tctgcctcat caaacagcaa
      481 tattaacgcg ccaaaacggc gacgcgtttt ctctcccaga tgaacaaatt caatctcaag
      541 tggtagcggc aggacatcgt tcattaacac atcccatagt gagtcgagat cgcgtacctt
      601 atctttcgcc agaccaaagg tttggctaaa gtcacgataa aaatcctcct gactctcaat
      661 ctcatcaaaa tcaaaggtat aaatattcat ctgttgccac cgtcacgttt cgcggcgagt
      721 tctgccgttc atcgctaagt atagccatga aaaaaccgac gcttttggcg tcggttttga
      781 cttaactatc ggtcaacgca tgttgatact tatggagcat ccccgccagc cgatgaaccg
      841 gttccgtcac ctgcggtggc gcttgccaga tgcgtagctt ttcctggtag atttcaagtt
      901 cttccagcaa ctggccaaag taccgacgac gtttatcatc gctacgggca gatatcacat
      961 ggtctgctgt gcggcgcatt tgtcggtgaa acgccgataa atcctcgtta accgggatcg
     1021 gcgcatcacg caggcgctgg tgcgcgataa tcatcgtcag cgccaggcga aatttcggca
     1081 aatcccctgg gaacttattc atcagcaaaa acagctgctg ataaagtgcc gggaggtgat
     1141 tctctttacg acgtgccaca ttggtagtca tcgcggaaac agcggcagaa acaaactgat
     1201 taagcagtac acgtccagtc ctgtcgcgcg atttatcccg caccagcaaa ataacggtga
     1261 acgcgagcac acagccgacg atttgcccta atgcgctgtc gagaaactga ctgaaatgga
     1321 aagtcatcgg gttatccagc acgataatat ttatggtgct ggccagcgcc cccatcgagc
     1381 ccagtcgccg tttctgtact tctataccga ggaaaaatcc cagcactgcc aggctaatac
     1441 acagcaataa catgctttgt tgggtattag ggataatcac caaaaagtag agcagcccta
     1501 acggcagcgc cgccagcgtc ccatagataa agtcgatcgc caccatgcgt ggattcggca
     1561 aacgcattgc cagtgacgtc acgaccgcaa tcatcaccat tgcaccactg ccggaagtcc
     1621 agcccgtcca cagccagaaa agcgtcccca gaatgcagga aagtgtggtt cgccagaagt
     1681 taaccatcgc atgatgacgt tcggctgact ctactttgac ttccggttcg ccttgcagga
     1741 tctcttcttc agtagcgttg atttttgtgt tactgataac gccgcgcttg agaagctgat
     1801 aacgcgttgc cgccgcgacc cagctataaa tggtgacagg cgtttcccgt tccccggtcc
     1861 aggcgataac tctccgcagg cgtttgagct gcttgtggac gtcctgcgcg gtttctaccg
     1921 gcgtgtcaaa aaattcgcgg aaagtatcag tgatcaattc cgggcgcgta ttctgaataa
     1981 gataagtttc gcaggattgg gtaatcagcg tcagcgatag cgtattgatc gcttttaaac
     2041 gtcgattggc ccgcgcccag cgggaagatt ccatattcag gttgctgcgc ataccttgta
     2101 acgccgtggt gcgtcgcacc agatcgcccc aggctttatc gacaacttca ccatcgccat
     2161 gtttgataca gagttgcatt aattgatatt gcgcgaccag caaactttcc agctctcgat
     2221 ccacttcttg tttgatcgat cgcggagaaa agagcaaatc cgccataatc gcacacacaa
     2281 taccgataac gatctcgcta caacgttcga cggcaaactg cggcgtaagc aatggttccg
     2341 gctgaatggt gatcacaatg atcagcgcgg tataaccggc cagcccccac gcatacgagt
     2401 tttctattcg taccagcgag gatatccagg tacaaaaacc ggcccagata cagcacacca
     2461 gaatcatcaa taatggtgcg cggatcatcg caatgatgat taccagtcca gcaatacagc
     2521 caataaatgt gccgatgatg cgcaaaaagc cacgatagcg aatagcgcca gaatacggtt
     2581 cacctcccgc agcaaaggcc ggaccggcgg caacaatcgc cgctgtcagt accgcccagc
     2641 gtggcgtttc cagctggaag tgaaagccaa caaacagcgc cagtacaatg gcggtcgcca
     2701 gttttaccgc aaagcgaata tgttgattag caatggagaa aatacccatc gtgattaacc
     2761 aaactcacgc aggcgatggg ccattttacg gaagaacgaa tcctggcttt cgtcgcgatc
     2821 ttgtttgcca gtgaccacca ctgtagcagt ggtgcccgca ggccagatgt tctcttgctg
     2881 gttgtcgaga cgaatacgta ccggaacacg ttgcgccaaa cgtacccatt caaggttaga
     2941 gtcgatggtc gccatccctt tgtcgtcacg cgtgctgctg gcgttggtga cccctgcggc
     3001 aacactatca acagtccctt tcagcacttt gttactgcca agcggcgtga tctccgcacg
     3061 atacccaggg cgcacccctt ccagcttagt ttcttccata taggccagta catagaagga
     3121 gttctgtttc accagcgcaa ccgccgttga tcctcgagta ataaactcac cgatatagac
     3181 gttgaggttg gtcacccagc catctgctgg cgcgcggatg actgtacgtt caagatccag
     3241 ttttgccaga tcacgggtcg cctgcgcttt cgctaactga tgcagaaccg tttgtagtac
     3301 gttgttggcc tgatcgatct cttcgcgaga catcgcctgc acaccgagac ggttacgacg
     3361 tcctgcctcc tggcgtttct cctgtgccag tacctgataa taagcaacat cggcttgcgc
     3421 ttcctcaagc gccttttgat agcgcggctg gtcgatggtg aacagtacct gtcctttttt
     3481 caccagctgg ttatcatgaa cattcacctg ggtaatgagt ccagaaacgt ccggcgcgat
     3541 cgcaacgacg tcagcgctaa agcgcgcgtc acgcgtccac ggggattcgg tgtaatagac
     3601 ccaggcatta aaaattgcga tgaaggccag aatgactaat acgaccgtga tggccgtacg
     3661 ggagaatttt cttattagtg ttttcacttc aacctcaaac gaacagtcgc gatatcaaat
     3721 aaaacaagca gcaatagagc gcggtgttga acaacgccgg atgccagaca aagtcgtaga
     3781 tacctgttgg cacaagtacc cggcgcacca gccagaaaat cgccagtgat aaaagcaatt
     3841 caaaaaatat cggtgggaag gacagcccaa acaccacgat aacgggaaac agactcatgt
     3901 tgaccttggt tgtatagaga gagcaggcgt tattattttc agcatctgtc gccgcagaga
     3961 agggcatgga aagccgggcg agagcaacat tgctgtagat tgatatttaa tatattagcg
     4021 taactgttat gctgttatct atattatgtg atctaaatca cttttaagtc agagtgaata
     4081 atggaacgac taaaacgcat gtcggtgttt gccaaagtag ttgaatttgg ctcttttacc
     4141 gccgccgcca gacagctaca gatgagcgtt tcgtccatca gtcagacggt gtcaaaactg
     4201 gaagatgagt tacaggtaaa gctgttaaac cgtagtacac gcagcattgg cctgactgaa
     4261 gccggcagaa tttactacca gggctgccgt cggatgctcc atgaagtgca ggatgttcat
     4321 gagcaactgt atgccttcaa taacaccccc atcgggacgc tgcgcattgg ctgttcttca
     4381 actatggcac aaaatgttct cgccgggctg acagcaaaaa tgctgaaaga atacccaggt
     4441 ttgagcgtca atctggttac cggaattcct gcccccgacc tgattgccga tggtctggat
     4501 gtggtgatcc gcgtcggcgc gttgcaggat tccagcctgt tttcccgccg tctgggcgcg
     4561 atgccgatgg tggtgtgcgc cgcgaaaagc tatctcacac aatacggcat accggaaaaa
     4621 cccgccgatt tgagtagtca ttcatggctt gaatacagcg tgcggcccga caatgaattt
     4681 gaactgatcg caccggaagg gatctcgact cgcctgatcc cacaaggaag atttgtgact
     4741 aatgatccga tgacgctggt gcgctggctg acggcgggtg ccgggatcgc ctacgtgccg
     4801 ctgatgtggg tgatcaacga gatcaatcgt ggggaactgg agatcctgct gccgcgttat
     4861 cagtcagatc cacgcccggt ttatgcgtta tataccgaaa aagataagct gccgctgaag
     4921 gtacaggtcg tgatcaactc gctgacggat tattttgttg aggtcggtaa attgtttcag
     4981 gagatgcacg ggcgcgggaa agagaagtaa ttcatattgt actgttacgt tgtacaaacc
     5041 tgtgccaacg ggttcccctc accctaaccc cagaggagcg aggggaccgt tcgtgcacgt
     5101 agaaagatta attatccttc tgaaaatact gaaattatta cgcagtaccg ccaacagtca
     5161 ggttatcgac tttcaacgtt ggctggccca cgccaaccgg caaactttgc ccttctttac
     5221 cgcagacacc cacgccgtta tccagtttca ggtcgttacc caccatcgaa atctgctgca
     5281 tggtttcgat accggaacca atcaacgttg cgcctttcac cggcttcgtt actttaccgt
     5341 tttcaatcag atatgcttct gaagtggaga aaacgaattt gccggaggtg atatccacct
     5401 gaccgccacc aaagttcggt gcatagatac cgtactcaac ggattcaata atttcctgcg
     5461 gggtcgattt acccggcagc atataggtgt tggtcatacg cggcatgggc agatgggcgt
     5521 aggattcacg gcgaccgttg ccagtcggcg tcatccccat caaacgcgcg ttgagtttat
     5581 cctgcatgta gcctttcagg atgccattct caatcagcac gttgtactgg cctggcgtac
     5641 cttcgtcatc aatcgccacc gaaccacggc gatcgaccat cgtgccatca tcaaccacgg
     5701 tacacagttc tgaagccacc aactccccga cctgtccact aaatactgaa gtgccacggc
     5761 ggttgaagtc gccttccaga ccgtgaccca ccgcttcatg caacagcacg cccggccaac
     5821 ctgcgccaag tactaccggc atggtgcccg ctggtgcagc aacggcagaa agattgacca
     5881 gcgccatacg cactgcttct tttgcccacg catccgcacg gacttcgcca tcgagatcgg
     5941 caaggaagaa ctcataacca aaacgaccgc cgccaccact ggcaccgcgt tcgcgtttgc
     6001 catcttcttc gaccaaaacg ctcacggaaa gacgcaccag cggacggaca tccgccgcta
     6061 gtgtgccgtc ggtggccgca accagaatta attcatagac accactgagg ctggcagtca
     6121 cttcctgtac gcgcttgtcc gcttcgcggg caaccttatc gacgcgacgc aggatatcca
     6181 acttctcttc acggctcatg ctttgcagcg gatctaccga ggtatataac gggctatgct
     6241 ctacagcgcc cagcgtctgt actttgccat caccactatc acggacgatg gtgcgcgccg
     6301 cttgtgcact ctgttccagc gccagcaggc tgatttggtc agcataagca aatccggttt
     6361 tttcaccact gattgcacgc acgccaacgc cctgatcgat gttgtaagaa ccatctttaa
     6421 taatacggtc ttctaaaacc caggattcgt gatagctcga ctgaaaatag agatcgccat
     6481 aatcaaggcg acgttcggcc agttgaccga ggatcgcgaa caagtcctga tgtttcaggc
     6541 cgttcgccgc tagcaattgt tcacttacca ggttcagact catcgttttt gctactcgtt
     6601 agttactgca gtagaggatt ttttacggct gccggatgcg gcgtgaacgc cctgtccggc
     6661 ctacggttat gttctggctc gttggccttt ggcaacgatt atcctatgag attggggcaa
     6721 ttacgcgccc tcgtcaaatc attgcgcttt ttctttacgc ggttgtcgca acacttcgtt
     6781 gatttgcgga tcgtccagcg gacccgaaat gtgatagcgc aaaatggaga ctttgctcca
     6841 cagcggtccc agcactttac tggcggcaaa cactgccgcg ccaacaatgg ggttaaccgc
     6901 aaaggccgca gccacaccca ccgtcgcaga aatctctggt gcgacaaccg cttccatatt
     6961 caggtcgcga cgtaccagat ttaccgaccc tttcatagcg atgtccgctt ccaggccatc
     7021 caccagcgtg tcgtcggtgt gcataacgcc gtctttaatc cacgcggtgc tgcgaatgga
     7081 gtcaaaatag aacccttcgc caaaagtgtc tctgaaatca aaacgcagct tacgcatcag
     7141 ggcatctacg ctcaataagc gcagcaattg cccggcatgt ccggtattga tttcggtaat
     7201 ttcgccttta cccagttgag tatgaatgat gccattcaac gtcgcctcat ctggttgcca
     7261 gggtgctttg cgccagtgta aatcgtaatc cacattaaat gacgactggc gtatgggcgt
     7321 cgtgacacca aaaaattctg cggcggcatc aattttctgc ccacgcagtt ttcctttcag
     7381 cgaggtgcgt tcattccccg gattgttaac ccattcacca tcggcagtga gccgcgaaaa
     7441 accagtatca atcagtccat tagtcagcgt taatgtattg ccagaaatgg tgatatcact
     7501 gtcaatgcgc ccgaattttt gcccccagaa ccagcactct gcgcatcgta tttgtgcgtc
     7561 cggccagcca cggaagctaa tgcgctccgt tgtcgggaaa ggtgatgaat tcgttgagtc
     7621 accatgagtt ttcgccacgc ttgggttgta ataaagatat ttgatattcg ccagccacgg
     7681 cgcgttatta cgcatcgcca gcgtggcgtt gatttcacgc ccttgcgcct caaccagggt
     7741 gccatttgcc gtcggttgcg aaacaatact caggttattc cattgctgat ttcccagcga
     7801 caacataggc gtacgtaacg ttatgtgttg tgggaaactc gctgcaccac cgacactctc
     7861 cgcagcgcct ttctggaaca gggccagcca ctcggcacca ttcatcggcg gcatattgag
     7921 ttcaacgcca ctttgttccg gcaacggcgg gagcgtttta ctgtctgccg cccaaatagc
     7981 acggtcgagc gtcagctttt gaccgagcaa ccagcggcta ttgaaatggt tatctgcacc
     8041 agcctgtccg gttaactcaa agctgttgag attgccataa accttaacgt ttaccggcag
     8101 tggttcaccc gcaggtttgg ctaacggtga aggtaagtga ctgctcacat tcttcaggtc
     8161 gccgttcagc tctacgttat aggtcgcgcc agcatgataa ggcagatcaa tgcccacttt
     8221 accatcccac gccacgctgc cacttaatgc ttcgttcacc gcttcaggca gaacgccggt
     8281 tttcgctggt tgccagttac cgttgagatt caccgcgacc tggtaggctt ttgccccttc
     8341 tttggtggaa aaatccacgt tcaacggctg attaaaccag cttgctgtca gtggttcact
     8401 ttgcagatcg ccattgataa agctgaattt accgctcaaa tttttcaggg tgctgtcgag
     8461 tggtttgata aacagactgt tattacgcag cgtcacatca cctttcgcgg ttaccagttc
     8521 gccgttcagc gggatatcaa gatgtaagcg agcattcaca tcgccgtcga gctggagttc
     8581 ttgcagggtc gcacccagag aatctttcag cggtgtctca tcaaagtaag ggccaacggc
     8641 tttacccgga cctttaatgt cagcgtcaat cagcagtttt tcttttgagt agtcagggat
     8701 cactgcggtg agattacttg cgcgcacgcc gcccagatta acgccatcgg ttttcatcca
     8761 taaaccgtcg ttaataaagt ccagttcaat atcaaggtta gttaatgcag gccagtccgg
     8821 ctggaaggca aacttcgcgt tgcgcagcgg taccagcact tcaaactgac cttcgttgtg
     8881 tttatagggg aagagttgcg gattgccacc ataaaccagc gtcgcgttat ccgcttcacc
     8941 gccctgaatc gcgccactta agtaatcaac caggtcttta cccatcaagt tttccgggaa
     9001 atagcgccag gcttgtgaac catcatcggt actgatgcca gccagaatac ccagccaggg
     9061 ttcatcgtta gctggttgca ggtaacgaaa accgccgcgc gcatggacgg ctttggcttt
     9121 aacgtcaata ttacgcccat ccagctggaa acctttgtca ttgttcagcc agcttatcgt
     9181 tgcctggccg tcggcgattt ccagtggcgc acggaatacc gtttcgtaag gcatctttgc
     9241 ctgcttcatc gacgcagtaa gcaaaccatt ttctacgctg ccggaaagcg tcccggagaa
     9301 gtgttccgca cccggtaata atttccattg cttccaggcc agatcgctcc acgatgcctg
     9361 aaaacgggtc ttgtctgccg cctgaagcgg gatatccagc gccagcgtgt taatcttgcc
     9421 gctcggttgt gtggagcgcc aaacatcacc cagtgcaggt gaaagtttcg cggccagcgg
     9481 gcgtatgccc tccaggcctg ccagctccag attactggcg cgaatccgga gttcgtcact
     9541 gcgtttattg tctttgccgc caacgtcctg ttccggtatc caggccagcg tcaatgctcc
     9601 gctcggccag ggtttgccgt ccatcgtgat ccgtgtatcg ggaatagaga actgccagcc
     9661 tggattttca cgcgtaatat gcgcagtcag attatccacc gacagcgtat gtgtttcctt
     9721 ctcgcccaac cagctggcac cgccctgttt cagccagacg tcaccgccgg ttacatcgcc
     9781 tttgtcgatc gtcatccagc cttcaaggga gaactgtgcc gtttccagcg caatattgtc
     9841 ctgcatccat ttaccgagcc acggcttcag gtcgatgtca tccgcctgga gccagacgcg
     9901 accattgctt aacaacccct catcatcgcg caaatccata cgcacctgca tcacgccgtg
     9961 ctgtccggta aggctggaga ggcttaccag gccttccgca cggtgtcgac gtggatcgtt
    10021 cagccaggtg agctgtggga tcgccagctc ggcgcgctga ccggatggcg tcaggaaact
    10081 gacttcactg tcgcggagat cgaaatggtc aaattgacga agaaacagat cgctgatgtg
    10141 actggcttcc aggctatcat taccaccgcc gctggtgata ggagtgttgg tgcgaaaacg
    10201 cagctgccag aaagtgaggt cgcgaaactg ccagcgcata tgtaacaggc tctgccagac
    10261 atccagcgcc agagtaacgc gtttaaccga aaattcgccg ccatctttta gttctgcacg
    10321 gatgtcgtgc gcttcaagcg tcggaccaaa attctgccag ctggctgaga gctgactggc
    10381 ttctaccggc atgccagtcg cggattctat tttgttgagg atttccggac gccaggcgtc
    10441 aagatgcggt aaagcaatac gcaggccgct aaccagcagg gcagcgatca caacgagcgc
    10501 ggctccagta agcagtaaaa tccccggcaa tcgcctcacc cgtcactcct tgtctgctaa
    10561 aaatgtgact caaaaaccct ttgccggatg gcggcccagc atctgtttac atcattacga
    10621 cgtcaaactg ctcctggtta tagagcggtt caatttgtac tttaacctgt ttaccaacga
    10681 aaatttccac ttccgccagc gagtgtgact cttcgccttt caaggcttca gctactgccg
    10741 gagaagcata gaccaggaaa cggtcggaat cataagcatg gtgtacacga acaatctcgc
    10801 gcatgatttc atagcatacc gtttccacgg ttttcaccgt tccgcgaccg tggcaggttg
    10861 ggcattcgtt acatagtacg tgctcaatgc tttcgcgggt gcgtttacgc gtcatctcca
    10921 ccagccccag cgccgaaaaa ccattaacgc tggttttcac ccggtctttg ctcaacgcct
    10981 gctccagcga gtgcagcacg cggcggcggt gatcttcatt attcatatcg atgaaatcaa
    11041 taatgataat cccgcccaga ttacgcaacc gtaactggcg agcgatagcc tgcgtcgctt
    11101 caatattggt attgaaaatg gtgtcgtcca gattgcgatg accgacaaac gccccggtat
    11161 tgatgtctac ggtggtcatc gcttcggtct ggtcgataat cagatagcca ccggatttca
    11221 gttctacttt gcgttccagc gctcgctgaa tttcgttttc gacatcaaag agatcgaaaa
    11281 tcggctggcg tccggtgtaa tgctccagct tgctggtcat ctcgggaatg tactccgagg
    11341 taaactccag caatgcttcg taagtcaggc gtgagtcaac gcgaatgcgg tcgagttcgg
    11401 cgtcggcgaa atcacgcaga acacgctgcg ccagcgccag ttcgccgtac agctgataac
    11461 gagtctgcgg gcgcttttta cgctccatca ctttggtcca gacgcgtttc agataagcgg
    11521 catcggaggc cagttcagcc tcgccaaccc cttcagctgc ggtacggatg ataaacccgc
    11581 cctgctcgtc gcaatactct gcgaccactt ttttcaggcg ttcacgttct gattcgcttt
    11641 caatacgttg ggaaacccca acgtgagaag cccctggcat aaacaccaga tagcgagaag
    11701 gcagcgtgat atcggtagtc aggcgcgcac ctttagtgcc aagcggatct ttcaccacct
    11761 gcaccatcag atcttgcccc tgacggacca gttccgagat gtcgcgtacc gtgaattgct
    11821 tttgttcttc acccgccaca cattcagtgt gcggcatgat atcggatgca tgaagaaacg
    11881 cggctttatc cagcccaata tctacaaaag ccgcctgcat acccggaagt acacgactta
    11941 cacgaccctt gtagatattg cctactattc cgcgtcgcgc ctcacgttca atatgaattt
    12001 cctgcagaat accgccatca atatacgcca ctcgcgtttc cgaaggcgtt acgtttacca
    12061 acaattcagc cgtcatgttt atcccttttc tcacgcagtg cgttaaaatt acttaataat
    12121 tcatacgttt caaccagcgg taagccgact acggcgtgat agctgccatt tatcttcctg
    12181 acaaaacagc cacccagccc ctgaataccg tatgcacctg ctttatctaa cggttcaccg
    12241 ctggcgacat agcccgcgat gtcttcgtct gttaacgttc tgaaagtcac atcggtgacc
    12301 accaggcaat cgagaatgtg ctggctgtcg gccaacgcca ctgccgtcat cacctgatgg
    12361 gtctgacccg ataatttgcg caacatctgc gccgcatgct ctgcgtcgcg cggtttctcg
    12421 agcacttctc cgttcaggat aacgatagta tccgcaccca gcaccgggag atcctgcgcc
    12481 gtttgcgcga cacccgcctg tgctttctcg cgcgccagac gcacaacata ctgctgcgcg
    12541 ctctcctgcg gctgacgctg ctcctcaatg cccgtaacaa tacgttcaaa ggtcacgcca
    12601 agttgcgcaa gtaactcctg acgacgcgga gaaccggaag ctaaatacag agaagtcata
    12661 gaaaccttta ttgcactgca aactgctgac ggactttacg catcagcaag aaaatccacg
    12721 gccagagcac cccattgact acactactcc agaacacttc cggtctgaaa gagacgttaa
    12781 tcactaaaaa ctctgcccag aaaacaataa tatccaccac cagcgaaagc aacatgacga
    12841 ccagcgcctg ctgccataat gcgaggttgc ggaaaagctg gtatttcagc gccaccaggt
    12901 aagcaatgat gctcatcgcc aatacgcgta cgccaagcgt cgagccgctg atcagatcca
    12961 gtatggcacc catcacaaaa cctgtgccca catttacgcg atggggcaag gccaggatcc
    13021 aatacaacaa gatgagtaac acccagtttg gccggaaaac aatcaggtta tccggccagg
    13081 gcatgatctg cagcaacagc gcaatgagga aagagagcca gattacccag cgtccctggc
    13141 tacgatagct cgccactatt gtcctcccgg cgcacgcgca ggcggttgag caccactttg
    13201 cggcggcgta gccctttgtg gagagcgatt agcagcgggc tgtgtgggcg cagcaggcgc
    13261 agtaactgca tttcctgtcg ccggttgctg cggagtcggc tgagtgatcc ccgttgccgg
    13321 ttcaggtaac tttggcccca tcgcgtctgg cgaaggcaat acctgcggca tcatctgcat
    13381 caaacgttca ttagcaacac gatgcacctc ttccggcgtc atcgggttag cgccgttacg
    13441 atccgcgccc cacagcagca gcagataacg caaacgttgc agtcctgcag tcggacgcgc
    13501 ctgaatcaca gtataagcgc gctgggtatc gagttttacg gaagagacaa ccgcgaccgg
    13561 atagccttcc gggaaacgac cgcccagacc ggaagtcacc agcacatcac caacacgaat
    13621 gtccgtattc gctggcagat gctcaagctg caaatcatcc gtacaaccgt taccggctgc
    13681 aattacgcgg atatcgttgc gcagcacctg gattggcagc gcgtgggtcg catcacaaat
    13741 cagcagcacg cggctggtca gtttagcgac ggccaccacc tgaccaacaa cacctttgtc
    13801 gctgataacc ggctggcctt cataaacgcc attaacgcta cctttatcga taacaacttg
    13861 atcgctataa ggatcgttaa ccgtggagat aacctgagtc accattttct gctcatcctg
    13921 acgcagcggg gaacccagta gctcgcgcag acgcgcgttc tcctgtttgt attgtccaag
    13981 catcagcagt tcactgtttt tcagcaacag ttcctgacgt aacgcccggt tttcaagttc
    14041 tagttggtca cgcgaggcca gcgtctgcga tacgccatcc agcaattcac gaggagcatt
    14101 ggaaacaaag tagaaaggac tgacggcggt atccatataa gtacggattt gactgaacgt
    14161 ccccaggcgg ctgtcggcaa taataatgcc gagcgccacc agcaccgcca gaataaggcg
    14221 aatctgtagc gacgggccac ggctaaaaat tggcttcata agttatgcgt attctcgtat
    14281 cagaccaggc agggtaaaca gacacttccc ctgcctgcat ccgattactc ttcgctgaac
    14341 aggtcgccgc cgtgcatgtc gatcatttcc agcgctttgc cgccaccgcg cgccacacag
    14401 gtcagcgggt cttcagcaac aacgactgga atgccggttt cttccattaa caaacggtca
    14461 aggttacgca gcagtgcgcc accaccggta agcaccatgc cacgctcgga gatgtcggaa
    14521 gccagttccg gcgggcactg ttccagtgca accattaccg cgctcacaat accggtcagc
    14581 ggttcctgca gtgcttcgag gatttcgttg gagttcaggg taaagccgcg tggaacacct
    14641 tctgcaaggt tacggccacg aacttcgatt tcacgtactt catcgcccgg gtaagccgaa
    14701 ccgatttcgt gcttgatacg ttctgcggtg gcttcaccga tcagagaacc gtaattacga
    14761 cgtacatagt tgatgatagc ttcgtcgaaa cggtcaccac caatgcgcac ggaagaggag
    14821 taaaccacgc cgttcaagga gataacagca acttcagtag taccaccacc gatatcaacc
    14881 accatagaac cggtcgcttc agaaaccggc aggccagcac caattgcggc agccatcggt
    14941 tcttcaatca ggaagacttc acgggcacca gcgccctgcg cggattcacg aattgcgcgg
    15001 cgttcaacct gggtcgcgcc aaccggcaca caaaccagaa cgcgcgggct tggacgcata
    15061 aagctgttgc tgtgcacttg tttgatgaag tgctggagca ttttttcagt cacgaagaag
    15121 tcggcgataa cgccgtcttt cattgggcga atggcagcaa tattgcccgg cgtacggccc
    15181 agcatctgct tcgcgtcatg acctactgca gctacgcttt tcggtgaacc ggcacgatcc
    15241 tgacgaatgg ccaccacgga aggctcattc aatacgatgc cttgtccttt tacataaatg
    15301 agggtattcg cagtacccag gtcaatggac aagtcattgg aaaacatgcc acgaaatttt
    15361 ttcaacatac taagggataa tcctgaaagc tggggcggaa aagaaaatcc gcttacttta
    15421 ccaaccacac gcagcagcga caaggcgcaa aaatcatctg ctacggtgaa aattagtgca
    15481 gttcgtttcc tttgttacaa atctctgcct gagtccagaa aggcttaatg catcagcagc
    15541 attcctcgcc tgtttgcaac cgcgtaaggt cattcatctg catatgtgct gcaacaatct
    15601 ggcgagcaga caagcacact cccatgagac gcagcgcgcg ttattctacg tgaaaacgga
    15661 ttaaacggca ggttaaaccg agtatctttg tgaatatttt ttcacgttag tatcaagtgg
    15721 ctgtgaggac gcgaaaaaat ccccttgccc acctgtaaca ccgcgctgaa tcagggtctg
    15781 ccactcgctt cgcgaacgca cgccggtggc gtaaacctgg gtgctggtcc cggagcaggc
    15841 ttccaccagg ctttgaacca gcagttggtt ctccgttcgc ttctcaatgt ttctgaccag
    15901 ccccggatgg agcttgagta actcaacatt aagttctttg atccaactgg tgcttaccag
    15961 cgtcaaacca gcctggttga cggctacccg tacccctaaa gcattcacta aacgaataac
    16021 aggttgtaag cgactgatat gttgaccaac atcggcctct gcaagttcaa taattatgtg
    16081 tcttcgttgc gatttttcac attgcattaa cgtatcgcgc agccaacgct gaaaacgcgg
    16141 gcgaatcagc gactcaacgg taacctgaat cgccagattt tcctctggcc agtaacgcaa
    16201 tagtggaata agacggctga tttgcaggcg gtcatactct tccgataaac caaactgcaa
    16261 gaccatcggc atatactctg ccgagctaac ctcttcatta ccatcgaaaa tgcggcacat
    16321 gagttcacga tgatgaaccc ggccttcgcg agtaaccgcc ggtttttgat aaaggcgcgg
    16381 gccgccgcga ctgagcattt gctcgataag cgtacgccag cgaacattac cgcgtccttt
    16441 ttcaggcaac gagtcatcgt aaatagccca gctattgccg ccctgcaatc ccgcattacg
    16501 cgtggcagac tctgcatgtt ccattacctg ctcggtatcc tgaccactac gccaggcaca
    16561 gataccaatg tggatcatat cgtcgcgatc gagcatttta ttgttcggca aggtatcgac
    16621 ggctttgatt aactgactgg cgatgctctc tgcctctttt aacgtccggt gcggtaacag
    16681 cgcagcaaaa tcactgcggt ggtaacgcgc cagcagtgcg ccagggtagc gcatcataaa
    16741 tgtcgatagc agattcgtca gagagaagaa ctgttcttca acctggctgt gcccccaggt
    16801 atcactcaac atattgaaat ccggcagacg aatcatcatc acgatcccgt gggtacctac
    16861 tttctcttga tcttccagta acgttgctaa ctgattatcg aaaaagagtc ggttattgag
    16921 gccggttttc atgtcctggg cggcataaga gcggatcagc gtatcaagac ggctgtgttg
    16981 ttcgcgtgcg ttctgaattt cacaaagcag cgtatccagc gcactgctgg ttctgggcgg
    17041 ccattcatag atggttccca acacattaga gccacgctca ccgtttaaga tacgagtagc
    17101 ccgggtttcc agcaattctt gcccggcaag ttgccgttgt aaccagcgta ccgccaggaa
    17161 gagcataaga atgataaagc caatcgcccc cgtgagcggc gcggtagtca ttaaagaatg
    17221 gaaatagttg cccatcggat cctgataaac cagacgcagc gacatccccg gatgctttat
    17281 caacggaacg ctcagttcac gaaacagatc gttggtgcca accggacgat aactaccatt
    17341 tctggccagg gtataaacct gcttatcacc atggagcagg tctacacgaa cgatatctgc
    17401 cgacatcatt aattcggtaa tttgtggcct taatgtgctg aagtcattcg acactaggtg
    17461 ggtatcaatc gccgtcgcca ccgcctgaac acgatgacta aacttatact gaatggcgtt
    17521 gtagaaactt agcgaacagc ccagcaaagt cacaaaaatt gttaacccgg tgagcagcgt
    17581 gacaaaggcc gaaaatttcg tcgttaatct catccttgtg ttaactccga tagtgaggaa
    17641 gcgggcatac tagcaaatca gatttatctc gcaatttatt gcgcttcatc ggctttgctt
    17701 ttccattagc gagtatagtc ttcagaaatt attttccaat ccatcatgca catgaggacc
    17761 acttatgcag gcgttacttt tagaacagca ggacggcaaa actctcgcat cagtacagac
    17821 tctggacgaa agtcgcctgc cggaaggcga tgtcacggtt gatgttcact ggtcgagcct
    17881 gaactacaaa gatgcgctgg cgattaccgg taagggaaaa atcatccgaa attttccgat
    17941 gattcctggg attgattttg ccggaactgt acgcaccagc gaagatccgc gttttcatgc
    18001 cggtcaggag gtgttactca ctggctgggg cgttggtgaa aaccactggg gtgggctggc
    18061 ggaacaggcg cgagtgaaag gtgactggct ggttgccatg ccgcaagggc tggacgcgcg
    18121 taaagcaatg attatcggta ctgccggttt taccgccatg ctgtgtgtga tggcgctgga
    18181 agatgccggt gttcgcccgc aggatgggga ggttgtcgtg acgggtgcca gtggtggcgt
    18241 cggcagtacc gccgtcacgc tgctgcataa gttgggttat caggtcgttg ccgtttccgg
    18301 tcgcgaaagt acccatgaat atctgaaaag tttaggtgcc agccgtattc ttcctcgcga
    18361 tgagtttgct gaatcccgtc ctctggaaaa acaagtctgg gctggggcaa ttgacaccgt
    18421 tggcgacaaa gtgctggcaa aagtgctggc gcaaatgaat tacggcggct gcgtggcggc
    18481 ctgtggtctg gcgggtggtt ttactctgcc aaccacggtc atgccattta ttctgcgtaa
    18541 tgtccgtttg caaggggtgg attcagtaat gacgccacca gcacgccgcg cacaagcctg
    18601 gcagcgactg gtcgccgatt taccggaatc attctatacc caagcggcaa aagagatatc
    18661 tctggcagag gcaccgaagt ttgccgaggc catcattaat aaccagatcc agggtcgcac
    18721 gctggtgaag gttaactaac catttagcag ggaataataa gagagggaac tcattttgaa
    18781 atcattattc aatcggttaa cgggaaaagc ggttagccgg acagctttcg tcgaacatct
    18841 tggtcatgaa gttgtacaac atcatccaaa ctggaaagtc atgatttcga ctgaccataa
    18901 attgatgcgc attgatgctc caccaaacag ccattattga tacgcctccg tcgcctgtta
    18961 ggtttatggt gttttgcctt ggcgacgcta cgcttaatct gttactgatt tctggtacca
    19021 tggggggaat aatctgattt tatttgattg caaattaacc acttaaacct attgaatgcg
    19081 gaacattgtc agtcggttaa ttttgcgtgc tttagcattc acatctttcc ggacgatgca
    19141 gtgaaaattg cgtaatcctc agcaaccgct gcgtaatgtc gtctatcttg tcgcgatcct
    19201 ggcatcccta cattatttgt ggtctgtgaa gattatctca ttgcagcccc tcatcttcgc
    19261 agggctggct ttacagcttt tcaccctacg ttataagaag ttccgtcgat gatggcgcta
    19321 atttcgtgaa ttgtgcggct tgttgcaaat tacacggtgt tgaaggttat ttacatgtta
    19381 gctgttgatt atcttccctg ataagaccag tatttagctg ccaattgcta cgaaatcgtt
    19441 ataatgtgcg acctcgtcct ccctgacgca gtttttgcgc tgcggaaaag gtgacattgg
    19501 cgcaacgaag gtatattttg ttttttgccg gaggatagca gcagatcgct gcacaatgtc
    19561 cgtcaagtct aacattgaca ctctggggca aaatagaccg gcgtcccggc ctgctggaat
    19621 ttatcgctat gcatacagct gtcggggcat acgctttaca gacggcggtg aaacgcctgt
    19681 cacaatcaca ctaaacaaag agtacggaac ccactcatgg atattcgtaa gattaaaaaa
    19741 ctgatcgagc tggttgaaga atcaggcatc tccgaactgg aaatttctga aggcgaagag
    19801 tcagtacgca ttagccgtgc agctcctgcc gcaagtttcc ctgtgatgca acaagcttac
    19861 gctgcaccaa tgatgcagca gccagctcaa tctaacgcag ccgctccggc gaccgttcct
    19921 tccatggaag cgccagcagc agcggaaatc agtggtcaca tcgtacgttc cccgatggtt
    19981 ggtactttct accgcacccc aagcccggac gcaaaagcgt tcatcgaagt gggtcagaaa
    20041 gtcaacgtgg gcgataccct gtgcatcgtt gaagccatga aaatgatgaa ccagatcgaa
    20101 gcggacaaat ccggtaccgt gaaagcaatt ctggtcgaaa gtggacaacc ggtagaattt
    20161 gacgagccgc tggtcgtcat cgagtaacga ggcgaacatg ctggataaaa ttgttattgc
    20221 caaccgcggc gagattgcat tgcgtattct tcgtgcctgt aaagaactgg gcatcaagac
    20281 tgtcgctgtg cactccagcg cggatcgcga tctaaaacac gtattactgg cagatgaaac
    20341 ggtctgtatc ggccctgctc cgtcagtaaa aagttatctg aacatcccgg caatcatcag
    20401 cgccgctgaa atcaccggcg cagtagcaat ccatccgggt tacggcttcc tctccgagaa
    20461 cgccaacttt gccgagcagg ttgaacgctc cggctttatc ttcatcggcc cgaaagcaga
    20521 aaccattcgc ctgatgggcg acaaagtatc cgcaatcgcg gcgatgaaaa aagcgggcgt
    20581 tccttgcgta ccgggttctg acggcccgct gggcgacgat atggataaaa accgcgccat
    20641 tgctaaacgc attggttatc cggtaattat caaagcctcc ggcggcggcg gcggtcgcgg
    20701 tatgcgcgta gtgcgcggcg acgctgaact ggcacaatcc atctccatga cccgtgcgga
    20761 agcgaaagct gctttcagca acgatatggt ttacatggag aaatacctgg aaaatcctcg
    20821 ccacgtcgag attcaggtac tggctgacgg tcagggcaac gctatctatc tggcggaacg
    20881 tgactgctcc atgcagcgcc gccaccagaa agtggtcgaa gaagcaccag caccgggcat
    20941 taccccggaa ctgcgtcgct acatcggcga acgttgcgct aaagcatgtg ttgatatcgg
    21001 ctatcgcggt gcgggtactt tcgagttcct gttcgaaaac ggcgagttct atttcatcga
    21061 aatgaacacc cgtattcagg tagaacaccc ggttactgaa atgatcaccg gcgttgacct
    21121 gatcaaagaa cagctgcgta tcgctgccgg tcaaccgctg tcgatcaagc aagaagaagt
    21181 tcacgttcgc ggccatgcgg tggaatgccg tatcaacgcc gaagatccga acaccttcct
    21241 gccaagcccg ggcaaaatca cccgtttcca cgcaccaggc ggttttggcg tgcgttggga
    21301 gtctcatatc tacgcgggct acaccgtacc gccgtactat gactcaatga tcggtaagct
    21361 gatttgctac ggtgaaaacc gtgacgtggc gattgcccgc atgaagaatg cgctgcagga
    21421 gctgatcatc gacggtatca aaaccaacgt tgatctgcag atccgcatca tgaatgacga
    21481 gaacttccag catggtggca ctaacatcca ctatctggag aaaaaactcg gtcttcagga
    21541 aaaataagac tgcaaaagcg tcaaaaggcc ggattttccg gcctttttta ttactgggga
    21601 tcgacaaccc ccataaggta caatccccgc tttcttcacc catcagggac aaaaaatgga
    21661 cactcgtttt gttcaggccc ataaagaggc gcgctgggcg ctggggctga cccttttgta
    21721 tctggcggta tggttagtag ccgctcactt acctggcgtt gcccccggtt ttaccggttt
    21781 tccgcgctgg tttgagatgg cctgcatcct gacgccgctg ctgtttattg gactgtgctg
    21841 ggcgatggtg aaatttatct atcgcgatat cccactggag gatgacgatg cagcttgaag
    21901 taattctacc gctggtcgcc tatctggtgg tggtgttcgg tatctctgtt tatgcgatgc
    21961 gtaaacggag cactggcacc ttccttaatg agtatttcct cggcagccgc tctatgggcg
    22021 gtattgtgct ggcgatgacg ctcaccgcga cctatatcag tgccagttcg tttatcggcg
    22081 ggcctggagc tgcttataaa tacggcctgg gctgggtatt gctggcgatg attcagcttc
    22141 ctgcagtctg gctttcgctc ggtattctcg gcaagaagtt cgcaattctt gcgcgccgct
    22201 acaatgcggt gacgctgaac gatatgctgt ttgcccgcta ccagagtcgt cttctggtgt
    22261 ggctggcgag tttgagtttg ctggttgcat tcgttggtgc gatgaccgtg cagtttatcg
    22321 gcggtgcgcg cctgctggaa accgcggcgg gtattcctta tgaaaccggg ctgctgattt
    22381 tcggtatcag cattgcgttg tataccgcct ttggcggctt tcgcgccagc gtgctgaacg
    22441 acaccatgca agggctggtg atgctgattg gcaccgttgt gctgcttatt ggcgtggtac
    22501 atgccgcagg tggtttaagt aatgcggtag acacgctgca aaccatcgat ccgcaactgg
    22561 ttacgccaca aggcgctgac gatattctgt cgcctgcctt tatgacgtcg ttctgggtac
    22621 tggtgtgctt tggcgttata ggcctgccgc atactgcggt gcgctgtatc tcttataaag
    22681 acagcaaagc cgtacatcgg gggatcatca tcggtacgat tgtggtcgca atcctgatgt
    22741 tcggtatgca tctggccgga gcgttaggtc gggcggtgat ccccgatctc accgtaccgg
    22801 atctggtgat cccgacgtta atggtaaaag tgctgccacc gtttgccgcc gggatcttcc
    22861 tggctgcacc gatggctgcg atcatgtcga caattaacgc ccaactgctg caaagttccg
    22921 ctacgatcat taaagatctc tatctgaata tccgtccgga tcaaatgcaa aacgagacgc
    22981 gtctgaagcg gatgtcggcg gtaattacgt tagttctcgg tgcgttgctg ctgcttgccg
    23041 cctggaagcc gccagaaatg atcatctggc tgaatttgct ggccttcggt gggctggaag
    23101 ccgttttcct gtggccgctg gtactgggtc tttactggga acgcgccaac gccaaaggcg
    23161 cgctaagtgc gatgattgtt ggcggcgtac tgtatgccgt actcgcgacg ctgaatattc
    23221 agtacctggg cttccaccct atcgtgccct cgttactact aagtttgctg gctttcctgg
    23281 tcggaaaccg tttcggtaca tccgtcccgc aagctaccgt tttgactact gataaataaa
    23341 gagttttgcc atgccttgga tccaactgaa actgaacacc accggcgcga acgcggaaga
    23401 tcttagcgat gcgctgatgg aagcgggtgc cgtttctatc acttttcagg atacccacga
    23461 tacgcctgtg tttgagccgc tgccgggcga aacgcgcctg tggggcgaca ccgatgtgat
    23521 tggtctgttc gacgctgaaa ccgatatgaa cgacgtggtg gcgattctgg aaaaccatcc
    23581 gctgctcggc gcgggcttcg cgcataaaat cgaacaactg gaagataaag actgggagcg
    23641 cgaatggatg gataatttcc acccgatgcg ctttggtgaa cggctgtgga tctgcccaag
    23701 ctggcgtgat gtgccggacg aaaacgccgt caacgtgatg ttagatccag ggctggcgtt
    23761 tggtacgggt acccatccaa ctacctctct gtgcctgcag tggctcgaca gcctcgattt
    23821 aaccggtaaa acagtcatcg actttggctg tggttccggc attctggcta tcgcggcgct
    23881 gaaactgggt gcagcaaaag ccattggtat tgatatcgat ccgcaggcga ttcaggccag
    23941 ccgcgacaac gccgaacgta atggcgtttc tgaccgtctg gaactctact taccgaaaga
    24001 tcagccagaa gaaatgaaag ccgacgtggt ggtcgctaac atcctcgcag gcccgttacg
    24061 tgaactggca ccgttaatca gcgtcctgcc ggtttcaggc ggtttgctgg gcctttccgg
    24121 tattctggca agccaggcag agagcgtttg cgaagcttat gccgacagct tcgctctgga
    24181 cccggtcgtg gcaaaagaag agtggtgccg cattaccggt cgtaagaatt aaccttcgca
    24241 tcgccgtagg gtgacgcaga gcgaagtgcg agcgatgtca caaaaggcac gttaatttgc
    24301 cgattattta cgcaaatttg cgtgccaaaa ttttcattca taaagaaaaa ttgagaactt
    24361 actcaaattt cttggagtgt aaattttagt cactattttc taatatgatg atttttatga
    24421 gtaattatcg cgccacgctc attttaaatg caattctttg atccatctca gaggattggt
    24481 caaagtttgg cctttcatct cgtgcaaaaa atgcgtaata tacgccgcct tgcagtcaca
    24541 gtatggtcat ttcttaactc atgcgcatcg gacaatatca gctcagaaat cgcctgatcg
    24601 cagcgcccat ggctggcatt acagacagac cttttcggac gttgtgctac gagatgggag
    24661 ccggattgac agtatccgag atgatgtctt ctaacccaca ggtttgggaa agcgacaaat
    24721 ctcgtttacg gatggtgcac attgatgaac ccggtattcg caccgtgcaa attgctggta
    24781 gcgatccgaa agaaatggca gatgcagcac gtattaacgt ggaaagcggt gcccagatta
    24841 ttgatatcaa tatgggttgc ccggctaaaa aagtgaatcg caagctcgca ggttcagccc
    24901 tcttgcagta cccggatgtc gttaaatcga tccttaccga ggtcgtcaat gcagtggacg
    24961 ttcctgttac cctgaaaatt cgcactggct gggcaccgga acaccgtaac tgcgaagaga
    25021 ttgcccaact ggctgaagac tgtggcattc aggctctgac cattcatggc cgtacacgcg
    25081 cctgtttgtt caatggagaa gctgagtacg acagtattcg ggcagttaag cagaaagttt
    25141 ccattccggt tatcgcgaat ggcgacatta ctgacccgct taaagccaga gctgtgctcg
    25201 actatacagg ggcggatgcc ctgatgatag gccgcgcagc tcagggaaga ccctggatct
    25261 ttcgggaaat ccagcattat ctggacactg gggagttgct gcccccgctg cctttggcag
    25321 aggttaagcg cttgctttgc gcgcacgttc gggaactgca tgacttttat ggtccggcaa
    25381 aagggtaccg aattgcacgt aaacacgttt cctggtatct ccaggaacac gctccaaatg
    25441 accagtttcg gcgcacattc aacgctattg aggatgccag cgaacagctg gaggcgttgg
    25501 aggcatactt cgaaaatttt gcgtaaacag aaataaagag ctgacagaac tatgttcgaa
    25561 caacgcgtaa attctgacgt actgaccgtt tctaccgtta actctcagga tcaggtaacc
    25621 caaaaacccc tgcgtgactc ggttaaacag gcactgaaga actattttgc tcaactgaac
    25681 ggtcaggatg tgaatgacct ctatgagctg gtactggctg aagtagaaca gcccctgttg
    25741 gacatggtga tgcaatacac ccgtggtaac cagacccgtg ctgcgctgat gatgggcatc
    25801 aaccgtggta cgctgcgtaa aaaattgaaa aaatacggca tgaactaatt caggttagct
    25861 aaatgcttga ttaaaaaggc gctactcggc atggggaagc gcctttttta taggtgtcac
    25921 aaagggagtg accatgagaa caggatgtga accgacccga tttggtaatg aagcaaagac
    25981 catcatccag ggtgatgcat taaccgaact taaaaagatc cccgccgaaa gtgtagatct
    26041 gatctttgcc gacccaccgt ataacatcgg taaaaatttt gatggtctga tcgaagcctg
    26101 gaaagaagat ctgtttatcg actggctgtt tgaagtgatt gcagagtgcc accacgttct
    26161 gaaaaagcag ggcagcatgt acatcatgaa cagcacggaa aacatgccct ttatcgatct
    26221 ccagtgccgc aagcttttta ccatcaaaag tcgcatcgtc tggtcatatg acagttctgg
    26281 agtgcaggcg aaaaaacact acggctccat gtacgaaccc atcctgatga tggtgaaaga
    26341 cgcaaagaac tacacgttca acggtgatgc tattctggta gaagccaaaa ccggatcgca
    26401 gcgcgcgttg atcgattatc gcaaaaatcc tccacagcct tacaatcatc aaaaagtacc
    26461 gggtaacgtc tgggattttc cgcgcgtgcg ttatttgatg gatgaatatg agaatcaccc
    26521 gacgcaaaaa ccggaagcct tactgaaacg cattattctc gcctcttcca acccaggcga
    26581 tatcgttctc gacccgtttg ccggtagctt tactaccggt gccgtagccg tcgccagtgg
    26641 gcgaaaattc attggtattg agatcaacag cgagtacatc aaaatggggc ttcgacggct
    26701 ggatgtcgcg tcgcattact ctgcggaaga actggcgaaa gtgaaaaaaa gaaagaccgg
    26761 caacctgtca aaacgaagca ggttaagcga agttgacccc gatctcattg caaagtaaag
    26821 gaagtgtaag cctggttttc agattattca tttcgtgtat atttcctgcc agacttggtt
    26881 aaacatgcac aggctcaggt aatgattcgc aagtattggt ggctcgtcgt tttcgctgtc
    26941 ttcgtttttc tgtttgatac tttactgatg cagtggattg aactgctggc aacagaaaca
    27001 gacaaatgcc gcaatatgaa ctcagttaat ccactaaaac tggttaactg tgacgaactg
    27061 aattttcacg acagaatgtg aatttatatg acacttaatt cattcgtttg atgaattaat
    27121 ttcgttatgt tttcatctgg catgaacatt cttaatacgt tatcgaccag agcgggggct
    27181 tgtttataaa gatcataacc cgccatattc attaaccagt tttgaacaat tccgctgaag
    27241 gcaccatcaa taataatcat cacaacatct aaatcgagat tatttgctat acaaccttgt
    27301 tgctgacacg cctgcaatac ttcgcggaga gtctgcggat taaagcccat cttttcgcgt
    27361 atcactccct cggccagcat ctcatcatta aattcacatt tgtgatataa gattttcagc
    27421 aacgcctgct ggcggggaat tttggcaata tattgcaaac cgacaatcaa tttttcacgc
    27481 aattgctgaa acgggtcatg ctctaaccca gcggtcaagt gatcctggat taactcccgc
    27541 aatgaaggct gttgcaacca catctcatta aacagttgag tcttgttctc gaagtgccag
    27601 tagatagcgc cacgcgtaac gttagcagcg tcggcaatgt cgttgagcgt cgtcttgctt
    27661 acgccatgct gcgcaaactg ggcgatggca gtttcaatca gttcttgccg ggtcttcagg
    27721 gcttcagctt tggttctttt tgccatgatt aattattcag gaaataaata tattcgacac
    27781 aggatgagaa aatagagaag gttaatctat cacctaatgt gtattta
//
LOCUS       DAPHCV010000066        27621 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.066, whole genome shotgun sequence.
ACCESSION   DAPHCV010000066 DAPHCV010000000
VERSION     DAPHCV010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.066"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            491..1558
                     /locus_tag="RH033_002762"
     CDS             491..1558
                     /locus_tag="RH033_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDV2991926.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            1555..4290
                     /gene="rbbA"
                     /locus_tag="RH033_002763"
     CDS             1555..4290
                     /gene="rbbA"
                     /locus_tag="RH033_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HDV2991927.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGSVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPPELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4290..5414
                     /locus_tag="RH033_002764"
     CDS             4290..5414
                     /locus_tag="RH033_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2991928.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQTVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            5487..5762
                     /locus_tag="RH033_002765"
     CDS             5487..5762
                     /locus_tag="RH033_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDV2991929.1"
                     /translation="MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            5759..6118
                     /locus_tag="RH033_002766"
     CDS             5759..6118
                     /locus_tag="RH033_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDV2991930.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLTNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(6238..6639)
                     /gene="nikR"
                     /locus_tag="RH033_002767"
     CDS             complement(6238..6639)
                     /gene="nikR"
                     /locus_tag="RH033_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HDV2991931.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(6645..7451)
                     /gene="nikE"
                     /locus_tag="RH033_002768"
     CDS             complement(6645..7451)
                     /gene="nikE"
                     /locus_tag="RH033_002768"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HDV2991932.1"
                     /translation="MTLLNVCDLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNICWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKAEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRCRTTEKV"
     gene            complement(7448..8212)
                     /gene="nikD"
                     /locus_tag="RH033_002769"
     CDS             complement(7448..8212)
                     /gene="nikD"
                     /locus_tag="RH033_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HDV2991933.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSL
                     TCAAALGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGK
                     IVEQGDVETLFNAPKHVVTRSLVSAHLALYGMELAS"
     gene            complement(8212..9045)
                     /gene="nikC"
                     /locus_tag="RH033_002770"
     CDS             complement(8212..9045)
                     /gene="nikC"
                     /locus_tag="RH033_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HDV2991934.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(9042..9986)
                     /gene="nikB"
                     /locus_tag="RH033_002771"
     CDS             complement(9042..9986)
                     /gene="nikB"
                     /locus_tag="RH033_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HDV2991935.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDHLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(9986..11560)
                     /gene="nikA"
                     /locus_tag="RH033_002772"
     CDS             complement(9986..11560)
                     /gene="nikA"
                     /locus_tag="RH033_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HDV2991936.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKIWTFTLRDGVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(11671..12258)
                     /gene="acpT"
                     /locus_tag="RH033_002773"
     CDS             complement(11671..12258)
                     /gene="acpT"
                     /locus_tag="RH033_002773"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HDV2991937.1"
                     /translation="MYRIVLGKVSTLSAAPLPPALRDQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLDNLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(12313..13362)
                     /locus_tag="RH033_002774"
     CDS             complement(12313..13362)
                     /locus_tag="RH033_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDV2991938.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            13461..14711
                     /locus_tag="RH033_002775"
     CDS             13461..14711
                     /locus_tag="RH033_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312349.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2991939.1"
                     /translation="MKHCCKNVVILMPEPVAEPALNGLRLNLRIVSIVMFNFASYLTI
                     GLPLAVLPGYVHDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLC
                     GCFLSGLGYLTAGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIG
                     RVISWNGIVTYGAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASK
                     GKPLPFRAVLGRVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFV
                     GTRLLFPNGINRIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFP
                     ALGVVAVKAVPQQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVA
                     IALLLTWRLKKRPPVEIPEAASSS"
     gene            complement(14715..15272)
                     /gene="dcrB"
                     /locus_tag="RH033_002776"
     CDS             complement(14715..15272)
                     /gene="dcrB"
                     /locus_tag="RH033_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HDV2991940.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADNQQKAQTTAENIINTLVIQ"
     gene            complement(15345..16010)
                     /locus_tag="RH033_002777"
     CDS             complement(15345..16010)
                     /locus_tag="RH033_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HDV2991941.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRHWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            16232..16477
                     /gene="tusA"
                     /locus_tag="RH033_002778"
     CDS             16232..16477
                     /gene="tusA"
                     /locus_tag="RH033_002778"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HDV2991942.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDELPYRYLIRKGG"
     gene            complement(16746..18944)
                     /gene="zntA"
                     /locus_tag="RH033_002779"
     CDS             complement(16746..18944)
                     /gene="zntA"
                     /locus_tag="RH033_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HDV2991943.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNTNDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQESRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSIAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAQSQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGVFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRK"
     gene            complement(19018..19644)
                     /locus_tag="RH033_002780"
     CDS             complement(19018..19644)
                     /locus_tag="RH033_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HDV2991944.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            19785..20144
                     /locus_tag="RH033_002781"
     CDS             19785..20144
                     /locus_tag="RH033_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HDV2991945.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFIGEQ"
     gene            complement(20147..20416)
                     /locus_tag="RH033_002782"
     CDS             complement(20147..20416)
                     /locus_tag="RH033_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HDV2991946.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(20406..21002)
                     /gene="rsmD"
                     /locus_tag="RH033_002783"
     CDS             complement(20406..21002)
                     /gene="rsmD"
                     /locus_tag="RH033_002783"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HDV2991947.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            21152..22648
                     /gene="ftsY"
                     /locus_tag="RH033_002784"
     CDS             21152..22648
                     /gene="ftsY"
                     /locus_tag="RH033_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDV2991948.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIAQAQEP
                     AKASEHAVEEQPQAHTEAEAETFAADVVEVSEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVIVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            22651..23319
                     /gene="ftsE"
                     /locus_tag="RH033_002785"
     CDS             22651..23319
                     /gene="ftsE"
                     /locus_tag="RH033_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDV2991949.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            23312..24370
                     /gene="ftsX"
                     /locus_tag="RH033_002786"
     CDS             23312..24370
                     /gene="ftsX"
                     /locus_tag="RH033_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HDV2991950.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            24615..25469
                     /gene="rpoH"
                     /locus_tag="RH033_002787"
     CDS             24615..25469
                     /gene="rpoH"
                     /locus_tag="RH033_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HDV2991951.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            25741..26844
                     /gene="livJ"
                     /locus_tag="RH033_002788"
     CDS             25741..26844
                     /gene="livJ"
                     /locus_tag="RH033_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HDV2991952.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(27032..27415)
                     /gene="panM"
                     /locus_tag="RH033_002789"
     CDS             complement(27032..27415)
                     /gene="panM"
                     /locus_tag="RH033_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HDV2991953.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDCGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         6521 a         7211 c         7677 g         6212 t
ORIGIN      
        1 atattttatt aaacccacga gcctttatca tctatcatct tttcagaata aattatttta
       61 tattcggtat ttattcgcac atcaggcgat aataacgcta tgtgatagtc atcaggtttc
      121 cctgttacgg aaacctgtgc aacagaatct gtgaacctgt acagaatatt tttggaaaaa
      181 acaccagcga acggaagaac atcgcgatgt atcacaagtt gatggcaatc aaatagacaa
      241 catgaatata actgttagta tttaattgat ttcacttatg agtatgcgtg tttcatttac
      301 ggccaggaat gctgatgatg acaacattac tacccgtttt cactaagcct tccccattgg
      361 cgctcaatgc tctgtgcgct ggtcgtattt gccatttcct tcttatcccg gatggaagaa
      421 tccgctgaac atcgttcggt ggcagttgta acacgcgtcg attgcttaca gggattatgg
      481 cggatcgatt atggataaga gtaagcgcca tctggcgtgg tgggttgtcg gggcactggc
      541 ggtggctgcc gtcgtggcgt ggtggctgtt gcgcccggcg ggtgtgccgg aaggctttgc
      601 tgtcagcaat gggcgcattg aagcgacgga agtggatatc gccagcaaaa ttgccgggcg
      661 tatcgacacc attctggtga aagaaggcca gtttgttcga gaaggtgaag tgctggcgaa
      721 gatggatact cgcgtgttgc aggaacagcg actggaagcc atcgcgcaaa tcaaagaagc
      781 acaaagcgcc gttgccgccg cgcaggcttt gctggagcaa cgccaaagcg aaactcgtgc
      841 cgcacagtcg ctggttaatc aacgccaggc cgaactggac tccgttgcca aacgtcatac
      901 gcgttcccgc tcgctcgccc aacgaggggc tatttctgcg caacagctgg atgacgatcg
      961 cgccgccgct gagagcgccc gagctgcgct ggaatcagca aaagcccagg tttcggcttc
     1021 taaagccgct atagaagcgg cacgcaccaa tatcattcag gcgcaaacac gcgtcgaagc
     1081 cgcacaagcc actgaacggc gcattgccgc agatatcgat gacagcgaac tgaaagcccc
     1141 gcgtgacgga cgcgtgcagt atcgggttgc cgagccaggg gaagtgctgg cggcaggcgg
     1201 tcgggtgctg aatatggtcg atctcagcga cgtctatatg accttctttc tgccaaccga
     1261 acaggcgggc acgctgaaac tgggcggtga agcacgtttg attcttgatg ctgcaccaga
     1321 tctgcgtatt cccgcgacca tcagttttgt cgccagcgtc gcccagttca cgccaaaaac
     1381 cgtcgaaacc agcgatgagc ggctgaaatt gatgttccgt gtcaaagcgc gtatcccacc
     1441 ggaattactc cagcagcatc tggaatatgt caaaaccgga ttgccgggcg tagcgtgggt
     1501 gcgggtgaat gaagaacttc cgtggcctga cgacctggtg gtgaggttgc cgcaatgacg
     1561 catctggaac tggttcccgt cccgcctgtc gcgcaactgg cgggcgtgag ccagcattat
     1621 ggaaaaaccg ttgcgctgaa caatatcaca ctcgatattc cggcccgttg catggtcggg
     1681 ctgattggac cggatggtgt cggtaaatcg agcttgttgt cgttgatttc cggtgcccgc
     1741 gtcattgagc agggcagcgt gatggtgctg ggcggcgata tgcgcgaccc gaagcatcgc
     1801 cgcgacgttt gcccgcgcat cgcctggatg ccgcaggggc tgggcaaaaa cctctaccac
     1861 accttgtcgg tgtatgaaaa cgtcgatttt ttcgctcgcc tgttcggtca cgacaaagcg
     1921 gagcgggaag tgcgaatcaa tgagctgctg accagcaccg ggttagcacc gtttcgtgat
     1981 cgtccggcag ggaaactctc cggcgggatg aagcaaaaac ttgggctgtg ctgtgcgtta
     2041 atccacgacc cggaactgtt gattcttgat gagccaacaa cgggggttga cccgctctcc
     2101 cgcgcccagt tctgggattt gatcgacagc attcgccagc ggcagagcaa tatgagcgtg
     2161 ctggtcgcca ccgcctacat ggaagaggcc gaacgcttcg actggctggt agcgatgaat
     2221 gccggagaag tgctggcaac cggcagcgcc gaagagctgc ggcagcaaac gcaaagtgcg
     2281 acgctggaag aggcgtttat aaatctgtta ccgcaagcgc aacgccaggc gcatcaggcg
     2341 gtagtgatcc caccgtatca accggaaaac gcagagattg ccatcgaagc gcgcgatctg
     2401 accatgcgtt ttggttcctt cgttgccgtt gatcacgtca atttccgcat tccacgcggg
     2461 gagatttttg gttttcttgg atcgaacggc tgcggtaaat ccaccaccat gaaaatgcta
     2521 accggactgc tgcccgccag cgaaggtgag gcgtggctgt ttgggcaacc ggttgatcca
     2581 aaagatatcg atacccgccg tcgggtaggc tatatgtcgc aggcgttttc gctctataac
     2641 gagctcaccg tgcggcaaaa cctggagtta catgcccgtt tgtttcacat cccggaagcg
     2701 gaaattcccg cgcgagtggc tgaaatgagc gagcgtttta agctcaacga cgtcgaagat
     2761 gtattgccgg agtcattgcc gctcggcatt cgccagcggc tatcgctggc ggtggcggtg
     2821 attcatcgcc cggagatgtt aatccttgat gaaccgactt ctggcgtcga tccggtggcg
     2881 agggatatgt tctggcagtt gatggtcgat ctctcgcgtc aggacaaagt gactatcttc
     2941 atctccaccc actttatgaa cgaagcggaa cgttgtgacc gtatctcact gatgcacgcc
     3001 ggaaaagtgc tcgccagcgg tacaccgccg gaactggttg agaaacgcgg agccgccagt
     3061 ctggaagagg catttatcgc ttatttgcag gaagcggcag ggcagagcaa cgaagctgaa
     3121 gcgccgccgg tggtacacga caccacccac gcgccacgtc agggattcag cctgcgccgt
     3181 ctgtttagct acagccgccg cgaagcgctg gaactgcgac gcgatccggt acgttcgacg
     3241 ctggcgctga tgggaacggt gatcctgatg ctgataatgg gttacggcat cagtatggat
     3301 gtggaaaacc tgcgctttgc ggtgcttgac cgcgatcaaa ccgtcagtag ccaggcgtgg
     3361 acgctcaatc tttccggttc ccgttacttt atcgaacagc caccgctcac cagttatgac
     3421 gagcttgatc gtcggatgcg tgcgggcgat atcacggtgg caattgagat ccctcccaat
     3481 ttcgggcgcg atatcgcgcg tggtacgccc gtggaactcg gtgtctggat cgacggtgcg
     3541 atgccgagcc gcgccgaaac ggtaaaaggc tacgtgcagg ccatgcacca gagctggcta
     3601 caggatgtgg cgagccgaca gtccacaccc gccagccaaa gcgggctgat gaatattgag
     3661 acgcgctatc gctataaccc ggatgtaaaa agcctgccag cgattgttcc ggcggtgatc
     3721 ccgcttctgc tgatgatgat cccgtcaatg ctaagcgctc ttagcgtggt gcgggaaaaa
     3781 gagctagggt cgattatcaa cctttacgtg acacccacca cccgtagcga atttttgctt
     3841 ggcaaacagc tgccgtacat cgcgctgggg atgctgaact ttttcctgct ctgcggcctg
     3901 tcggtgtttg tgtttggcgt accgcataaa ggtagtttcc tgacgctcac cctggcggcg
     3961 ctgctgtata tcatcattgc caccggaatg gggctgctga tctccacctt tatgaaaagc
     4021 cagattgccg ccattttcgg tacggcgatt atcacgttga tcccggcgac acagttttcc
     4081 gggatgatcg atccggtagc ttcgctggaa ggtccaggac gctggatcgg cgaggtttac
     4141 ccgaccagtc attttctgac tatcgcccgc gggacgttct cgaaagcgct ggatctgact
     4201 gatttgtggc aactttttat cccgctgctg atagccatcc cgctggtgat gggcttaagc
     4261 atcctgctgc tgaaaaaaca ggagggatga tgcgccattt acgcaatatt tttaatctgg
     4321 gtatcaaaga gttgcgcagt ctgctcggtg ataaagcgat gctgacgttg attgttttct
     4381 cgtttacggt atcggtgtat tcgtcagcga ccgtcacgcc aggatcgttg aacctcgcgc
     4441 cgatcgccat agccgatatg gatcaatcgc agttgtcgaa ccggatcgtt aacagcttct
     4501 atcgcccgtg gtttttgcca ccggagatga tcaccgccga tgagatggat gccggactgg
     4561 acgccggacg ctataccttc gcgataaata ttccgcctaa ttttcagcgt gatgtcctcg
     4621 ccggacgcca gccggatatt caggtgaacg tcgatgccac gcgcatgagc caggcattca
     4681 ctggcaacgg ttatatccag aatattatca acggtgaagt gaacagcttt gtcgcgcgct
     4741 accgtgataa tagcgaaccg ttggtatcgc tggaaacccg gatgcgcttt aacccgaacc
     4801 tcgatcccgc gtggtttggc ggggtgatgg cgatcatcaa caacattacc atgctggcga
     4861 ttgtgctgac cggatcggcg ctgatccgcg agcgcgaaca cggcacggtg gaacacttac
     4921 tggtgatgcc gataacgccg tttgagatca tgatggcgaa ggtctggtcg atggggttgg
     4981 tggtgctggt ggtgtcggga ttatcgctgg tactgatggt gaaaggcgtg ctgggcgtac
     5041 cgattgaagg ctcgatcccg ctgtttatgc tgggcgtggc gctcagtctg tttgccacta
     5101 cgtcgatcgg catttttatg gggacgatag cgcgttcaat gccgcaactg gggctgctgg
     5161 tgattctggt gctgctaccg ctgcaaatgc tttccggtgg atccacgccg cgcgaaagta
     5221 tgccgcagac ggtgcaggac attatgctga ccatgccgac gacacacttt gtcagcctcg
     5281 cgcaggccat tctctaccgt ggtgccggat tcgaaatcgt ctggccgcag ttcctgacgc
     5341 tgatggcaat tggcggcgca tttttcacca tcgcactgct gcgattcagg aagacgattg
     5401 ggacaatggc gtaaggaagg gaaacttgac ttcatattgt gatgtcatac tattgatgtc
     5461 ataaagtgaa gtcataagga catgccatga gaacccaggt acaggcttta cggaagaaac
     5521 aaaaaaatac attggatcaa atatttaaat ctcctgttcc tcaagggatt aagtggtctg
     5581 atatagagtc actggttaaa gcattaggcg gagaaattaa ggaaggaaga ggttcgcgtt
     5641 gtaagttcat actaaatatg agcgttgcgt gtttccatcg gcctcatccg tcgccagata
     5701 ccgataaagg cgctgtagaa agcgttcgtg actggttact aagtataggg gtaaaaccat
     5761 gatcaaactg aagacaccaa actctatgga gattgccggg caacctgcag ttatcactta
     5821 cgtgccagaa ctgaatgcat ttcgtggcaa attcctgggc ttgtcaggtt attgtgattt
     5881 tgtctctgac agtattcagg ggctgcaaaa agaaggggag ctttccctgc gagagtatct
     5941 tgaggattgt aaagctgcgg gtattgagcc gtatgcgcgt acagaaaaga ttaagacctt
     6001 tacattacgc tatccagaat cgctgagtga acgccttact aatgcggctg cgcagcaaca
     6061 agtctctgta aatacttata ttattgaaac gctaaatgag cgtttgaatc atttataaat
     6121 tttagcggcg tacattacgc agaatatctg cctttacagg atctatctat gcaactgaac
     6181 ttaattttat aaattaagtt cagttaatat cttttctttc ctcgttttag aaataactca
     6241 atcttccttc ggcaagcact gcaaatgccc gtgccgcacg ccgcgctggg cgataacgtc
     6301 atcggcaaaa tgctgcacgt cgcccatgtc acctttcagc acggcaattt ccagacagtc
     6361 gtcgtgatta atgtgcacat gcagagtagc aaccgagaga tcgtggtgat gatgctgggt
     6421 ggaaacaata cggctggcta agtcgcgttt ttcgtgttca tacacatagg agagcactgc
     6481 gaaaccttgt gtgccgtgct gctgggtggc ctcttgcgcc agggcgctac gcagaatatc
     6541 gcggatagct tcggaacggt tgttataacc acgacgctgg ctcaggctgt ccagcgtctc
     6601 cagtaaatcg tcatcaagcg tgatggtgac tcgttgcatt tgcgttaaac cttttctgtg
     6661 gtgcgacagc gcacggggaa tgcgggtaat accgcgtttt gtagcacacg tccggcgtca
     6721 gaggaaaagg ttaatttatc tcccaccacc tgggtttcga cgatttgccc gttgtccatc
     6781 accatcactc gttggcaaaa gcgttctacc aggcgtaagt cgtgggtgat gaacaggcag
     6841 gcggtgccaa actgttgttg cagctttttc agcaggcgaa tgacgcccgc ctgtaacacg
     6901 agatcaaggt tagaaacggc ttcatccaga atcagcagtt tcggttcgac cgccaacgcg
     6961 cgtgccaggc agacgcgctg gagctggccg ccgcttaact gcggtgggcg tttgtcgaga
     7021 acgctgtcat cgagatcgac cgccttcagc atttcgctgg cgcgcgccag ttgttcggct
     7081 tttttcagtg atagcaggtg gcgcatcggt tcacgcagga tctcgcgcac ggttttgcgc
     7141 ggattcacgg cactgatgga gtcctgaaac accatctgaa tatcacggcg gaaggcttta
     7201 cgttgggcgc gattaagttt cgccagcgat tcgccgcgcc agcaaatatt cccttgtgag
     7261 ggagactcta agcctaccag cagccgcgcg agggtgcttt ttccgcagcc gctgcgtccc
     7321 agcagggcga cggtttcacc gcttttcaga gtcaaagaaa cgttattcag caccgcttgg
     7381 tgctgatgtt ttccgctaaa tccaccgtgc gcatagtgat gtgaaaggtc gcagacgtta
     7441 agtaaagtca tgatgccagc tccataccgt agagggcgag atgagcggaa accaggctgc
     7501 gcgttaccac atgtttgggg gcgttaaaca gcgtttcgac atcgccctgt tcgacaattt
     7561 taccgtgcga catcaccgcc acatcatccg ccagacgcgc cacaacgccc atatcatggg
     7621 tcaccagcag cattcccggc gcttgttttt gcataatgct ctccagcaga tcgaggatgc
     7681 gcgcctgcgc taccacatca aggtcggtag tcggttcatc ggcgatgata aacggtgatt
     7741 cgcacagcac cgccatcgca atcatcatgc gctgcaacat gccgccgctc atctcgaacg
     7801 ggtacagctt cagcacgcgc gcggcgtttt ccagccccac cgcttctatg gcagcggtaa
     7861 gcgtggcgtc atcggcgggt ttccccagcg ctaggcaggt ttcccgcgcg tgggtgtgca
     7921 tggtgtgcag cggattaaag gcgctgcgcg ggttctgcat gatggtggca attttgatgc
     7981 cgcgcagggc gcagggagaa accggtttgc catcggctaa aatttccccc gccgtctggc
     8041 gaacgcctgc ggggagaatg cccagcgccg cggcgcaggt taatgatttc ccgctgccgc
     8101 taccgccgac taacgccagc acacgcccgc gtttaagggt taacgatacg ccgtgcacca
     8161 gcggctgcgc ggcctgtagc gcgatattac gtagttcaat ctgttgcggc attagtgtgc
     8221 gtgctccgtc accagatgag ggtccagatg atcgcgcagt gcgtcaccca ccaggttaaa
     8281 ggccatcacg ctgataaaca gcgccagccc cggccagaac atttgcagcg gctgggtcca
     8341 gatatactgg cgcgcgtcgt taatcatcac gccccattcg gcggtcggcg cggtcacgcc
     8401 gaggccgagg aaggacatcc ccgcgacgtg cagcatcata tgaccaatat ccagcgtcgc
     8461 cagcaccagc agcgagggga tcaccgcgcc tgccagatga tcgacaaaca cccgcacatg
     8521 gcccgcgccg gaaagccgtg acgccagcac aaactcgcgc tggcgtagcg aaatcaccag
     8581 gctgcgcacc atgcgcgcat accacgccca gtgcgacagg gcgatggcga taatcacgtt
     8641 ggtcaacccg gtgccgagta cgccgaccat aaagaacgac agaatcgacg tcgggaaggt
     8701 cataaacata tcggcaacgc gcatggtggc ctgatcaacg cgcccgccaa tcagcccggc
     8761 gctgccgcca ataactagcc ctaatgtcag caccagcagc aggcaggcca ttaccgagcc
     8821 gagcgacacg cgggtcgccg ccatcagccg cgagaaaata tcgcgcccta aatgatcggt
     8881 acccagccag tgctgcgcat ccggtgaaag caggcgcgac ggcagatcaa tcgcctgtgg
     8941 gtcatacggc agccaccact ggctggtgag cgcaatcagc gccagcaggg cgataatgat
     9001 cagcgccagg cgtaccgacc agcgggaaga gaggaaaaag ttcacgcgtg cgctccttca
     9061 tgacgacgaa tgcgcgggtc cagcgcggcg ttgagtaaat caacaatcaa attacagacc
     9121 acaaaaacca ctaccatcat cagcgtaaag cactggatca ccggatagtc acggttaaaa
     9181 atcgccgaca ccgcatagcg cccgacgcca ggccaggcaa agatgttttc gataatcatc
     9241 gtcccgccaa tcagttcgcc aatgtgcatc cccacggcgg tgatcatcgg cagcgaggca
     9301 ttgcgcagaa tatggcggcg ttcggtctgt ttgtcgttca ggccgcgtag ccgtgcccag
     9361 gtgacgtgac gctgaccggc gacgtccagc atactggcgc gcagcagacg cgcgttaatc
     9421 gccagcgaca taaaggcaat ggaaaccgca ggcagaatga tgtgctgcca gccgccgtag
     9481 cccatcgcgg gtagccattg cagatatacc gaaaacgcca tcaccagcaa aaacgccagc
     9541 cagaagttgg gcatcgacac gccgagaaac gcgatgaaac gtacggcgaa atccggcaga
     9601 tgatcgcgat ggcgcgccgc ccagataccg agcggcacgg aagtgagcag aattaatacc
     9661 agcgcggcac ccgcaagttc cagcgtggcg ggcaggaagt tcagcatatc gtccagcacc
     9721 gggcgttggc tggcgaatga gataccgaaa tcgagatgca gcgccttcca tagccaggtg
     9781 ccgtattgca cgtacagcgg ctgatccagc cccagcatgg tgcgggtaga ggccagcatc
     9841 tccggcgtcg gcggcaggtt agacagacgc aaataatcaa gcgccgggtc gccggtgccg
     9901 aggcgcagca tcagaaaaat gatcaccgag gcggcgagca ccatcgggat cagcagcaga
     9961 aagcggcgta atacgtaacg caacattaag gtttcaccgg tttaatctgt tcgaacggaa
    10021 tttcggtggc gatcggcgcg taggggatgt tacccagctc cggttttgat accaccatca
    10081 tcgagatgta gctgataggc agataaaccg cctcgtcatg cagacgggtc agaatgtcgc
    10141 gatacagcgc ctgacgttgc gtttcgtcat gggttgccag cacttcgccg atctctttat
    10201 caatcagcgg cttgtcggct aatccttgct gtgcctggaa atcagcgtgc gacggtacgc
    10261 gcattgaact caggaaggcg tgtggatcgt atggcgcgcc ccaggtgcga tggaaaatca
    10321 tgccaaaacg accgtcgcgc tgacgagcat agatactgct ctcttcttcg ccaatcagtg
    10381 agacatctgc gccaatctgg cgcatatcag cctgaatgat ttccgccatc gatttgctta
    10441 acgcatcggt gccgatgaac gacagttcaa tgcgcagcgg ctgaccattt ttctcgcgga
    10501 tgtctttgcc cgcaggcagc gtccagccgg ctttttccag caacgctttc gctttctgcg
    10561 gatcgtactg gcgcggtttc aggccgaggt tggcataggg cacagaagga gcaaacaagg
    10621 tgtcggcgac ctgctgggtg ccatacaacg cgttatcaat cagcgatttt ttgtttaccg
    10681 cgtaattaag agcttcacgt acggccagct cgttggtggg ggctttggcg gtattgagtg
    10741 ccagcatcac ggtttcgatc ggctgtgaca gttgggtgtg gtaagccggg ttctggctaa
    10801 agcgggcgaa ggtatcgagc ggtaataatc cttcatttcc gtacagcagg tcgatatcgc
    10861 cagtttcaaa cgctaccgcg cgggtagtcg ggtccgggat gacgttaaaa gtgatcttct
    10921 taatcgccgg cttctcgccc cagtagtttt cgttacggac aaagacatcg tactgattca
    10981 gtttcgattc ctgtaaaacc cacggcccgg tgccaatcgg cgctttaatt ccgttcatgg
    11041 tttcatggtt tttaaactgc gagggggcga taaagcggaa agggcgcggc agggccagtt
    11101 cttgcaggaa aggatagtag gcgcttttca gggtaatttg cagctctgtt ttactgagtg
    11161 ctttaacatc aacaatctgg tttgccagct ccagccaggc gtgacgttga cggttatcga
    11221 gcactgcgcg gaagttttct gccgccgctt ctgcatcaaa cggttcaccg ttggagaatt
    11281 tcacgccatc acgcagggtg aaggtccaga ttttgccatc ttcagaatga gtccagcttt
    11341 ttgccagcca cgggatcacc gaaccgtctg cctgatattt caccaatggt tcataaacca
    11401 tgctctgggc gaacatctgg ttaggcgtgt aaaggtgcgg gtttagtggc ccgacattca
    11461 ccggccaggc ggtggtgatt tcatctggtg cagcggcatg gacgataaaa gacgcacaag
    11521 ccagcagcgc aaatagagtg cggcggagtg tggagagcat ggtaacccca atggattaaa
    11581 atagatggcg gaaataagta tgacgatttt aagtattcgt catactgatt acctgttctg
    11641 gatcaataga tgggcagtcg ggtgggcgga tcagttaact gaatcgatcc attgcacact
    11701 gtcggcggtg agcgtaaagg gggtaggggt gcagatcgcc aggcttaaat tgtctagctg
    11761 acaatggctg actgacagtg atgagtgata ggtgctgtct acgctgacga tttgccaggc
    11821 gctgccgcca cgctgtttaa cgatggcttc tttgcgcgtc cagatgcgcc agaacatttc
    11881 cagttgctga tcaggatgca cggcgtccat ctcggcatgt tccccaaggc tgaacacggc
    11941 gttcgccagc cagcgccagt tggcgcgcgg gcgaatcact tcgatatcgc agccaacttc
    12001 gccttcatcg ctcaacagca gggcgatatc gtcaccgcta tggcttaagt tgaaccatag
    12061 cggcgtttcc ggcgcgaatg caggtttgcc ttgttcgcca tagatgatct ccggtagcgg
    12121 ggaaagcgtg tgcgaaagca atgcacgccc cgccagccag cgttcgcgtc gtggaccttg
    12181 cggtgcttga tcgcgtaaag ccggtggcag tggagctgcg cttaaggtcg aaactttccc
    12241 cagaactatc cgatacatat cagggccaac gtataatgga aaatgaaagt gcgtatcgta
    12301 acacttgtcg cctcatcccg gtaaccgact tttcggtctg cctggcccca gtaaaatcgc
    12361 cagtttgcta ccgcctttgg tggtttccat ccagatttta cacacgctgg ttaatggcac
    12421 cgaaagtagc atccctaccg ggccgagcag ccatccccaa attaacaatg aaagaaatac
    12481 caccatggtg gacatcccca ggcgatggcc catcatccgt ggctctaaaa tattgccgat
    12541 gaccatatgg acaactaaaa acaatgcgcc gaccagaata cattcgtaaa taccattaaa
    12601 cagcagcacc tgaatcattg gcggtacggc ggaaattacc gcgccgatat tgggcacgta
    12661 gttgagcaaa aacgccagta ctgcccacat cagcgcaaac tgcaccccca tcagctccag
    12721 ccccagccag acgatgacac ccgtccataa actgagtagc gtctttaacg ccagataatg
    12781 cgaaacgcct ttaagtgcgc ggtgtagtcc ggcgatgtga atctgtgggt tatttagcgc
    12841 aaaacgcatt ttgtaaggga cgtggcgcac ttcaaacagc atgaaaacta cggtcatcac
    12901 cagcaaaagc acgctcgcca ttgccccgga aagcccggtc attagcgccg tggtgaaggt
    12961 aaccactttt tccgagtcca tccgctgcag cattcgctcc ggcgacatat gtaaattaag
    13021 aaaaggcaac atctcctgca atttaaaaag tttgcgcgtc agctccttat taaacttcgg
    13081 cagcatagag ataaattcgt taaacgatgc cgccagtacg ccgaccagcg cagttagtgc
    13141 gatcagcatc accaccacca caatcgtaat ggcaacgggg cgttgtactc cccgacgaat
    13201 aaaccaggtg acgagcgggt tgaggacgat ggcaaaaaac agcgccagta acagctgcac
    13261 aatgatatct gccgctgcgt gaatgcccgc gaggatcact accagcgagg ccagcttgag
    13321 cagaatgtgc atgcccgttt tatcgggttg aggggtttcc attggggctt ccttgtgact
    13381 ttttgtatta agtgtagcgg gagtcacgcc agcaatattc tgattcttac tgctgatttt
    13441 cacgtcagca cgtggtaaaa atgaaacact gttgtaaaaa tgtggtgatc ctcatgcccg
    13501 aacccgtagc cgaacccgcg ctaaacggat tgcgcctgaa tttgcgcatt gtctccattg
    13561 tcatgtttaa cttcgccagc tacctcacca tcgggttgcc gctcgctgta ttaccgggct
    13621 atgtccatga tgtgatgggc tttagcgctt tttgggcagg attggttatc agcctgcaat
    13681 atttcgccac cttgctgagc cgccctcatg ccggacgtta cgccgatttg ctgggaccca
    13741 aaaagattgt cgtcttcggt ttatgcggct gctttttgag cggtctggga tatctgacgg
    13801 caggattaac cgccagtctg cccgtcatca gcctgttatt actgtgcctg gggcgcgtca
    13861 tccttgggat tgggcaaagt tttgccggaa cgggatcgac cctgtggggc gttggcgtgg
    13921 ttggctcgct gcatatcggg cgggtgattt cgtggaacgg cattgtcact tacggggcga
    13981 tggcgatggg tgcgccgtta ggtgtcgtgt tttatcactg gggcggcttg caggcgttag
    14041 cgttaatcat tatgggcgtg gcgctggtgg ccattttgtt ggcgatcccg cgtccgacgg
    14101 taaaagccag taaaggcaaa ccgctgccgt ttcgcgcggt gcttgggcgc gtctggctgt
    14161 acggtatggc gctggcactg gcttccgccg gatttggcgt tatcgccacc tttatcacgc
    14221 tgttttatga cgctaaaggt tgggacggtg cggctttcgc gctgacgctg tttagctgtg
    14281 cgtttgtcgg tacgcgtttg ttattcccta acggcattaa ccgtatcggc ggcttaaacg
    14341 tagcgatgat ttgctttagc gttgagataa tcggcctgct actggttggc gtggcgacta
    14401 tgccgtggat ggcgaaaatc ggcgtcttac tggcgggggc agggttttcg ctggtgttcc
    14461 cggcattggg cgtagtggcg gtaaaagcgg ttccgcagca aaatcagggg gcggcgctgg
    14521 caacttacac cgtatttatg gatttatcgc ttggcgtgac cggaccactg gctgggctgg
    14581 tgatgagttg ggcgggcgtt ccggtgattt atctggcggc ggcgggactg gtcgcaatcg
    14641 cgttattact gacgtggcga ttaaaaaaac ggcccccggt ggaaatacct gaggccgcct
    14701 catcatctta aaacttactg aataactagc gtattaatga tgttttctgc ggtggtctgt
    14761 gctttttgct gattgtcagc tggcagcgta atttgcatgg ttagcagttg attacccacg
    14821 ttacccagaa taacggaaga gtacgccgtc tggcctttcg cggagataat gctgtctaac
    14881 tgctgcattt tgtgaccttt cagctcaatg gctttattgg ttaccacttg cagctgcgga
    14941 tcgcggctac gttgctgatc ttccagacgc ttcgccagca ccgccagatc ttctttcgga
    15001 tcatcgccca tgatgacgat gactgctttc tgcccggtgg cgtcggacca gacatgcatg
    15061 ttattggcct gcgttcccag cttaccgctc tggtcggtca tatctgctgg cagcgagaaa
    15121 cttaacttgc catcaagtag gttgacggga ttcccggtcg cgttactttc tgcgaccgaa
    15181 ccctgcgccg tagcgttagt gtctttatca tcacaggccg caagccccat aaccagcagg
    15241 ccaattccga catatttaac cagattgcgc attgacttct tcctttcgat aaacggccat
    15301 aacggctcat tcatccatct tatcacaact ctgataacga acctttaact cgcctgcaaa
    15361 gcgttgattt cggatttatc tgccagtctt ttcaacagca tattgagtaa tacgccatac
    15421 attggcagga agaaaacgat actgattaac actttgaaac agtaatcgac cagcgcgatt
    15481 tccatccagt gttcagccat aaaggcatcc gggctacgcc agaaggcaat gaagaagaag
    15541 gccagcgtgt cgctgacgtt accgaacagt gtggacgctg tcggtgccag ccaccagtga
    15601 cgactctgac gcaggcggtt aaaaacgtgc acgtcgagga tctgccccag cgcgtaggcc
    15661 atgaaactgg cggtggcgat acgggcgaca aacaggttga agtgggcgag cgcgccgaat
    15721 ccctgccagg aacccatata gaacagcgac gagatgacgt aggagatcag taacgccggg
    15781 atcatcaccg caaagatgat acgacgggcc agcggtgcgc caaaaatacg cacggtcagg
    15841 tcggtagcaa ggaaaataaa cggaaagcta aatgcgcccc aggtggtatg gaaaccaaaa
    15901 atggagacgg gaagctgaac cagatagtta ctggaggtga tcaccagcag atgaaataac
    15961 gataaccaga acaacgcctt atagcgttga gtttgcgaga aaacgttcat attgtacctt
    16021 tttgattaac cattggggtg agggaaccca atacgtacga cacgctattt taccttgatt
    16081 aaataacgtt cttacccttt tttcgagccg ccgcatgata ctgctttacg ttgacaatgc
    16141 aatggttaat tttcacgcaa tcgttaacct ggtttgctta cggactcaca gggcgtaaac
    16201 tagcgccgtt tttttatgtg atgagaagaa aatgaccgat ctcttttcca gccctgacca
    16261 cacactcgac gcacttggcc tgcgctgccc ggaaccagta atgatggtgc gcaaaacagt
    16321 gcgcaatatg cagccaggcg aaacgttgct gattatcgcc gacgatccgg ccactacccg
    16381 cgatattcct gggttttgta cctttatgga acacgaactg gttgctaaag agacggatga
    16441 actgccttat cgttatttga ttcgcaaagg cggttgatgg ggaaagattg gcttcgatgc
    16501 cgccttttcc cctcacccta accctctccc cagaggagcg aggggaccga tcgcgctcaa
    16561 tgttgctgct tgaaaccggt ttgtaggccg gataaggcgt tcaagctgca tctggcgata
    16621 cggtgcacaa tgcctgatgc gacgctgtcg cgtcttatca tgcctacgag ttcgatgctc
    16681 tgtgtaggcc ggataaggtg ttcacgccgc atccggcatt gttggaccgt agactgatag
    16741 cctctttatt tcctgcgcaa taaccgtaac gcattcgccg ttaccagcac cgtcgcaccc
    16801 gtatctgcca gcaccgccag ccacaggccg gtcatcccta acagcgtggt gacgaggaaa
    16861 acgcctttca gccccagcgc aatagtgatg ttctggcgaa tattcgcgtg agtggcgcgc
    16921 gccagttcaa tcatttgcac caggccgcgc aggtggttat gcgtcaacgc cgcgtcggcg
    16981 gtttccagtg cgacatctgt gccactgccc attgcgatcc cgatggcggc agctttcatc
    17041 gctggcgcgt cgttaatacc atcaccgacc atcgccagcg gcgcatgttg attcagctcg
    17101 gtcaccgctt tgactttatc ttccggcaag aggcccgctt taaattccag ccccagttcc
    17161 ccggcgattg ccgctgctgc gcgtggatta tcgcctgtaa ggatcacccc tttgacgccc
    17221 agcgcgttca gttcactgat agcagttgca gcatcggcgc gcagggtatc ctgcaacgca
    17281 atgacaccca gcacgtcatc attacgtact accagcacta ccgtttgccc ggcgctttcc
    17341 agttcattaa tcaaaccagc aaatgcatca gcaggatgtt ttcctgcagc gcatatcaac
    17401 acgcgctcac cgttaacctg cgcttcaatg ccagacccga ccagcgcccg ctgtgattgg
    17461 gcggtgggaa tggcgagttc agcaacctgt gcttcgcgca caatggcctg tgccagtgga
    17521 tgcgtcgcgc cttgctcgac cgccgccgcc agtgtcagca gttcagattc actaataccc
    17581 gttgccggat gaatcgcggt aacgcgcggt ttaccgacgg tcagcgtacc ggttttatca
    17641 aacgccacct gggtaacacg acccagctgc tccagcgccg cgccgccttt aatcaacgcc
    17701 ccacgacgcg ctgctgccgc cagcccggag gtaatcgccg cgggcgtgga gataactaaa
    17761 gcacacgggc aaccaatcag cagcagcgtc agccctttat aaatccactc ctgccagctg
    17821 gcggcaaaca gcagcggcgg caccagcgtt accagcaggg cgacggccat aatcgctggc
    17881 gtgtaaatac ggctgaaacg gtcgataaac cgctcaatag gtgcgcggcg ctcttcggct
    17941 tcttcaatca gtttcagaat ccggtcaatg gcgctggcac ccggttctga cagcacttcc
    18001 agcgttacca gacggtctac gctggtggca cctgcaggaa ctttatcgcc cgtagcgcgc
    18061 tccacaggaa tggattctcc ggtcagggca ctttcatcaa aactggcaaa cggtgagagc
    18121 agtttaccgt cggcaggcaa acgcccacct gccgcaactt caatcacatc gccaggccgc
    18181 aggctgttta ttgccacctc ttcacgctca ccgttacgca gacgcgtggc ggtttctggc
    18241 ttcagcgcca ttaacgcgct aacgccctga cgcgcgcggc tggcggccca gccttccagt
    18301 cgttcaccaa tcaaaaacag cagcaacacc atcgcagctt cagccgttgc gccaataaac
    18361 agcgccccaa tagcggctat gctcattaag gtttcaatgg cgaagtagct gccggatttg
    18421 atcagccgta atgcctgacg agcaatcggg tacagcccaa ccagcgtggt cgcgataaac
    18481 gccagttgcc cgaacggatg attaaactgc tccagacccc agctgattgc catcatcacg
    18541 attagcgtaa tcagcggcag attctctttc aggcgtgatt cttgtggttc gtcggcggcc
    18601 tgttcatcgc gcagggaata gcccgctttt tgcaccgcag attcaacttg tgcccggatg
    18661 tcattgtcgg catcaaccac cagtttttcg gtggcgaaca gtacctgcac ctgattcacg
    18721 cctgcaagct ggcgcacggc attttccact ttgcgcgcac aggcggcgca gtccatgccg
    18781 ctgactttcc agctatagcg ggtgccggag acgttttcag agagagttgg cgtgctggaa
    18841 catgcgccgt cgcagcaaca gtcgttggtg ttctgtaccg tagttagcgg tttgaacgca
    18901 gcaaattgag gggctttctt gccgtgattg tcaggagtcg acatggcatc ctccggttaa
    18961 gttttttctc attaaccgaa ggatacactc tggagtcgac tccagagtca agttttatca
    19021 gagatacagc gagcggacga tgaggaaatg accggcaaag tagcaggccg cagcaatcgc
    19081 gttatctgcc cggaagcggc ggcgatagtg gctacccagc cagacaaagt tactgataaa
    19141 cagcaatgaa gcgccaacaa acgcagagag cgccggagcg gtcggacgga agaaccaaag
    19201 ttcacctgcc agccacacca tcaccagcgt catgccgata aacgtgcaga taggccaacg
    19261 gtactcttcc agccgcgtcc agataatcgc cagtaacagc gcacccagaa ccagcaacac
    19321 cagtggcaga ggccagaaga aagagagcgt catctgactg gcgaaataga tggtgtacag
    19381 caggtgcgag aggaaaaacg cgccgatggc gtacatcaga cgttgacgtg gcaacagggt
    19441 tagcgcatcg cccagcagtg aggcgcacag ccctgccagc accagatagc taatggcgtc
    19501 gaacatcggc gcttgccagg ccagtaacag caggagaaga agggttaacg gtttaaacac
    19561 ccagcgttgc caggttggcc cacgatacga tgcatccaca gatagccatg cggaaagaca
    19621 gacagcgata aacgaccaaa gcatcttagt tccttgaatt cgttattttt tcgtaagcgg
    19681 aatgcctacg gtcttgcttc cagtttagtg gcgagggtgg acggatgaca acaccataac
    19741 tggcagggta tgcttatttc cgcattttcc gatgagggat aaagatgagc aaaccacctc
    19801 ttttctttat tgttatcatt ggcttaatca tcgtcgccgc atcgtttcgt tttatgcaac
    19861 agcgacggga aaaagctgat aatgatatgg caccgctcca gcaaaagctg gtggtggtga
    19921 gcaacaagcg ggaaaaaccg attaacgatc gccgttcgcg ccagcaggaa gtgactccgg
    19981 caggcaccag tatgcgctat gaggcaagct tcaaaccgca aagcggagga atggagcaga
    20041 cgtttcgcct cgacgcccag cagtaccacg ccctgacagt gggcgataaa ggtacgctga
    20101 gctataaagg aacgcgcttt gtcagcttta taggcgaaca ataaggttat tttttaactt
    20161 taaatttctt ctgccagacc agcagttcaa acacgccgaa aaggaaaatc cgcacctttt
    20221 cggcggtggt catctgcggg ccatctttcg gtaaagtgga tttcaatagc gccagttgca
    20281 taccatgcat cagcacggta aaaatcagcg ccacgttaac gaaaatattc agcggacgcg
    20341 ggaagggatg caccaggttg aggattaaaa atccccaaac gcagagcatt aacaaacgac
    20401 caatattaat cagcatcact ttctccttgt gcttcgcgtt gatacagtcg ataagccacc
    20461 tgacccgcca ctttttcccg atgtaatgac cagttcgccg gaacagtggg cagaccgttt
    20521 tcgacttcgc tttcgacata aatcagggct tcgtcagcca gccagccgtt atcttccagt
    20581 aaatttatcg tctcttctaa caagccacgg cggaacggtg gatcgacaaa cacgatatta
    20641 tgcggtgtgc ctttttgcgc caggaatgac atcgcgttgc tgttcaccac gcgcgcattg
    20701 cctgctttaa gtgtcgccag attcttaatt aactgctgag aaaccgcgcg atccatctca
    20761 atcaacgtgg cccccgcagc gtagcgcgat aacgcttcca gccccagcgc gccgctcccg
    20821 gcgaagcaat ccagacattg ggcatcaaca atgaccggag ccagccagtt aaacaacgtt
    20881 tcgcgtacgc ggtcggtggt ggggcgcaga cctgggctat caggaaccgg gagtttacgg
    20941 cctcgccact gcccgccaat aatgcggatt tggccgctgc cggaatgatt cggttttttc
    21001 atgataaatt gctcaatccg ccagataaca catacttgca ggcggtgatg tgaattaagt
    21061 taagtgatag actatttcat cattttttta gctgctatgt acatagcgtt aacgctgtgc
    21121 catgaagcaa cagcgaggag tgtagtcgca aatggcgaaa gaaaaaaaac gtggcttttt
    21181 ttcctggctg ggctttggtc aaaaagagca gaccccggaa aaagagacag aagttcagaa
    21241 tgaacaaccg gttgtagaag aaatcgctca ggcgcaagag cctgcgaagg cttctgaaca
    21301 cgccgttgaa gagcagccgc aggcgcatac tgaagccgag gcggaaactt ttgctgccga
    21361 cgttgtggaa gtctctgaac aggttgctga aagtgaaaaa gcgcagcctg aagcggaagt
    21421 cgttgcacag ccggaaccgg tcgtagaaga aacgccggag ccagtggcta tcgagcgtga
    21481 agagctgccg ttgccggaag acgtcaacgc cgaagaggtt tcgccagaag agtggcaggc
    21541 tgaagcggaa accgtagaga ttgtcgaagc ggcggaagaa gaagcggcta aagaagaaat
    21601 caccgacgaa gagctggaag cacaggcgct ggctgccgaa gcggcagaag aagcggtgat
    21661 agtggttcct ccggcagaag aagagcagcc ggtggaagaa atcgctcagg agcaggaaaa
    21721 accaaccaaa gaaggtttct tcgcgcgcct gaaacgcagc ctgttaaaaa ccaaagaaaa
    21781 cctcggttcc ggatttatca gcctgttccg cggtaaaaaa atcgacgatg atctgtttga
    21841 agagctggaa gaacagctgt tgatcgccga tgtgggtgtg gaaaccacac gtaaaattat
    21901 caccaatctg acggaaggcg catcccgcaa gcagcttcgt gatgccgagg cgctttatgg
    21961 cctgctgaaa gaagagatgg gcgagattct ggcgaaagtc gatgagccgc tgaatgttga
    22021 aggtaaaacg ccgttcgtga tcctgatggt gggcgtcaac ggtgtgggta aaaccacgac
    22081 gattggtaag ctggcacgtc agtttgagca gcagggtaaa tcggtgatgc tggcggcggg
    22141 cgatactttc cgtgcggcag cggttgaaca gcttcaggtc tggggtcagc gcaacaatat
    22201 tccggtgatt gcccagcata ccggggcgga ttccgcctct gttatcttcg acgccattca
    22261 ggcagctaaa gcgcgtaata tcgacgtcct gattgccgat accgccggac gcctgcagaa
    22321 caaatcgcac ctgatggaag agttgaagaa aatcgtccgc gtgatgaaga aactcgacgt
    22381 tgaagcgccg catgaagtta tgctgactat tgatgccagc accgggcaga acgcggtaag
    22441 ccaggccaaa ctgttccatg aagccgttgg cttaaccggc atcacgctaa cgaaactgga
    22501 cggcacggcg aaaggcggag taattttctc ggtggctgac cagtttggta tccctatccg
    22561 ctacattggt gtcggcgaac gtattgagga tttgcgtccg tttaaggcgg acgactttat
    22621 agaggcactt tttgcccgag aggattaaca atgattcgct ttgaacatgt cagcaaggct
    22681 tatctcggtg ggagacaggc gctgcagggc gttacgttcc atatgcagcc gggtgagatg
    22741 gcgtttctga ccggtcattc cggcgcaggg aaaagtaccc tcctgaagct gatctgtggg
    22801 attgagcggc ccagcgccgg gaaaatctgg tttagcggcc atgacatcac gcgtctgaaa
    22861 aaccgtgaag ttccgtttct gcgccgccag attggtatga ttttccagga tcaccatcta
    22921 ctgatggacc gtactgtcta cgataacgtg gcgatcccgc tgattatcgc cggtgccagc
    22981 ggtgacgata ttcgtcgccg ggtgtcggcg gcactggata aagtcgggct actggacaaa
    23041 gcgaagaact tccctattca gctttcgggc ggtgaacaac aacgtgttgg cattgcccgc
    23101 gcggtggtga acaagcccgc ggtactgcta gcggacgaac caactggtaa cctggacgac
    23161 gcgttgtcgg aaggcatttt acgtctgttt gaagagttta accgcgttgg ggtaaccgta
    23221 ttgatggcaa cgcacgacat caacctgatc tcgcggcgtt cctatcgcat gctcaccctg
    23281 agcgatggtc acttgcatgg aggcgtgggc catgaataag cgcgatgcaa tcaatcatat
    23341 tcggcagttt ggcgggcgtc tggatcgctt ccgtaaatcg gtcggcggct caggcgacgg
    23401 cggtcgtaac gcgccgaaac gcgcgaaatc ctcgccaaaa ccggtaaatc gcaaaaccaa
    23461 cgttttcaac gaacaggtac gctatgcctt ccacggcgca ttgcaggatc tgaaaagcaa
    23521 accgttcgcc acgtttttaa cggtgatggt tatcgccatc tccctgacgc tgcctagcgt
    23581 ctgttatatg gtgtacaaaa acgttaacca ggcggcgacg caatattatc cgtcaccgca
    23641 aatcactgtt tatctgcaaa aaacgctgga tgatgacgcc gctgcgggcg tggtggcaca
    23701 gttgcaggcc gagcaaggcg tggagaaagt gaattatctt tctcgtgaag acgcactggg
    23761 cgagttccgt aactggtctg gttttggtgg tgcgctggat atgctggaag aaaacccgct
    23821 tccggcagtg gcggtggtga tcccgaaact cgattttcag gggacggaat cactcaatac
    23881 gctgcgtgat cgtatcaccc agatcaacgg cattgacgaa gtgcgaatgg atgacagctg
    23941 gtttgcccgt ctggcggcgt tgaccgggct ggtcgggcgc gtttcggcaa tgatcggcgt
    24001 attgatggtg gcggcagtgt tcctcgtcat cggcaacagt gtgcgcctga gcatatttgc
    24061 tcgccgtgac tccattaacg tacagaaact gattggtgcg acagatggat tcatcctgcg
    24121 cccgttcctg tacggtggcg cattgctggg attttctggc gcattgctgt cattaatttt
    24181 gtcagaaatt ctggtactgc gtttgtcatc ggcggttgcg gaagtggcac aggttttcgg
    24241 aacaaagttt gatatcaatg gcttatcgtt cgatgaatgc ctgctgttgc tgctggtatg
    24301 ctcgatgatt ggctgggtgg cagcgtggct tgccacggta caacatttac gccactttac
    24361 gccggaataa taaaagcgtg ttatactctt tccctgcaat gggttccgta gcagggaaag
    24421 agaccccgtt gtctcttccc ggtatttcat ctctatgtca cattttgtgc gtaatttatt
    24481 cacaagcttg cattgaactt gtggataaaa tcacggtctg ataaaacagt gaatgataac
    24541 ctcgttgctc ttaagctctg gcacagttgt tgctaccact gaagcgccag aagatatcga
    24601 ttgagaggat ttgaatgact gacaaaatgc aaagtttagc tttagcccca gttggcaacc
    24661 tggattccta catccgggca gctaacgcgt ggccgatgtt gtcggctgac gaggagcggg
    24721 cgctggctga aaagctgcat taccatggcg atctggaagc agctaaaacg ctgatcctgt
    24781 ctcacctgcg gtttgttgtt catattgctc gtaattatgc gggctatggc ctgccacagg
    24841 cggatttgat tcaggaaggt aacatcggcc tgatgaaagc agtgcgccgt ttcaacccgg
    24901 aagtgggtgt gcgccttgtc tccttcgccg tccactggat caaagcagag atccacgaat
    24961 acgttctgcg taactggcgt atcgtcaaag ttgcgaccac caaagcgcag cgcaaactgt
    25021 tcttcaacct gcgtaaaacc aagcagcgtc tgggctggtt taaccaggat gaagtcgaaa
    25081 tggtggcccg tgaactgggc gtaaccagca aagacgtacg tgagatggag tcgcgtatgg
    25141 cggcacagga catgaccttt gacctgtctt ccgacgacga ttccgacagc cagccgatgg
    25201 ctccggtgct ctatctgcag gataaatcat ctaactttgc tgacggcatc gaagatgata
    25261 actgggaaga gcaggcggca aaccgtctga ccgacgcgat gcaaggtctg gacgagcgta
    25321 gccaggatat catccgcgcg cgctggctgg atgaagacaa caagtccacg ttgcaggaac
    25381 tggctgaccg ttacggtgtt tccgctgagc gtgtgcgcca gctggaaaag aatgcgatga
    25441 aaaaacttcg cgctgccatt gaagcgtaat ttccgctact cagcagagaa ccctggatga
    25501 gaatccgggg tttttgtttt ttgggcctct gcaataatca gtttccccct ccggcaaaac
    25561 gccaatcccc acgcagattg ttaataaact gtcaaaatag ctattccaat atcataaaaa
    25621 tcgggtatgt tttagcagag tatgctgcta aagcacgggt agtcatgcat aaaacgaaat
    25681 aaagtgctga acaacaacat cacaacacac gtaataacca gaagaatggg gattctcagg
    25741 atgaacataa agggtaaagc gttactggca ggatgtatcg cgctggcatt cagcaatatg
    25801 gctctggcag aagatattaa agtcgccgtc gtaggcgcaa tgtccggtcc ggtggcgcag
    25861 tacggtgacc aggagtttac tggcgcagag caggcggttg cggatatcaa tgctaaaggt
    25921 ggtatcaaag gcaacaaact gcaaatcgta aaatatgacg atgcctgtga cccgaaacag
    25981 gcggttgcgg tggcgaacaa agtcgttaac gacggcatta aatacgtgat tggtcacctc
    26041 tgctcttcat caacgcagcc agcgtctgac atctacgaag acgaaggcat tttaatgatc
    26101 accccagcgg caaccgcacc agagctgacc gcccgtggct atcagctgat cctgcgcacc
    26161 accggcctgg actccgacca ggggccgacg gcggcgaaat atattcttga gaaagtgaaa
    26221 ccgcagcgta ttgctatcgt tcacgacaaa cagcaatacg gcgaaggtct ggcgcgagcg
    26281 gtgcaggacg gcctgaagaa aggcaatgct aacgtggtgt tctttgatgg tatcaccgcc
    26341 ggggaaaaag atttctcaac gctggtggcg cgtctgaaga aagagaatat cgacttcgtt
    26401 tactacggcg gttatcaccc ggaaatgggg caaatcctgc gtcaggcacg cgcggcaggg
    26461 ctgaaaactc agtttatggg gccggaaggc gtggctaacg tctcgctgtc taacattgcg
    26521 ggcgaatcag cggaagggct actggtgacc aagccgaaga actacgatca ggttccggcg
    26581 aacaaaccca ttgttgacgc gatcaaagcg aaaaaacagg acccaagcgg cgcgttcgtc
    26641 tggaccacct acgccgcgct gcaatctttg caggcgggcc tgaatcagtc tgacgatccg
    26701 gcggagatcg ctaaatacct gaaagcgaac tcggttgata cggtgatggg gccgctgacc
    26761 tgggatgaga aaggcgatct gaaaggcttt gagttcggtg tgtttgactg gcacgccaac
    26821 ggcacggcga ctgatgcgaa gtaatcatta atcggcaact ttgggttgcc gccaaattgc
    26881 taatatcgag tacgttgctt catgccggat gcggcgtaaa cgccttatcc ggcctacaag
    26941 attcaaagaa atcagtaaat tgcagcacac attgtaggcc tgataagcgt agcgcatcag
    27001 gcaatatcac ttgtgaaatc aaacttgacg attaacgctt ctcccagccg ccctgttgtg
    27061 ccgtaaatcc cagtgcctgc ataaacgctg tcatcacgcc gcaatcttcc acgcctgcat
    27121 ccgccatcca ccagcatgaa acgccaggat tgttacgcaa aacctcttcc agcagatatt
    27181 gccccacacc gcgacgtcgg gtgacttccc gcacgcgtag agaatccagt gccccctcgg
    27241 tgccgcttaa ggttacccgc acggccgcga gaaggcgctc gttaaaacgc gcggcgtaga
    27301 tacggtggtt atcgtcaacc tgtaaagagg aaggggaata ctccggccag atcttttgca
    27361 ggtcaatccg gtcctggtcg ctaaagtttt ctaatcgaat gatggtcagc ttcatgggga
    27421 acccgtgtaa atcacaaaag tggaaccagt gtagcgaaat aatttaatcg gaggctttct
    27481 cttttttaat tcttttggca ggtgattaat tttttaacag caataattac aaagttaaaa
    27541 cattagagaa tgaaaaatac ccagcataat cccctgaatg gtagtgaatt attccgccct
    27601 ttgtaccgtt attttatgct g
//
LOCUS       DAPHCV010000067        27593 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.067, whole genome shotgun sequence.
ACCESSION   DAPHCV010000067 DAPHCV010000000
VERSION     DAPHCV010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.067"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(107..709)
                     /locus_tag="RH033_002790"
     CDS             complement(107..709)
                     /locus_tag="RH033_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDV2991954.1"
                     /translation="MNTSPWNKDRIIGQKRPLQISHIWGIRIRLELEGKTRDLALFNM
                     ALDSKLRGCDLVKLKVSDVAYGSSVSSRATVLQQKTGSPVQFEITKGTREAVSALIKL
                     GNLRSKDYLFRSRVGTNQHISTRQYNRIFHGWVAKLGLEDSLYSTHSLRRTKPYLIYK
                     KTKNLRVIQLLLGHKKLESTVRYLGIEVDDALEISESIEV"
     gene            1108..2208
                     /locus_tag="RH033_002791"
     CDS             1108..2208
                     /locus_tag="RH033_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hsdR"
                     /protein_id="HDV2991955.1"
                     /translation="MFDVDDTAPDTPAPRELSQDVQALINNGLDFLDKAREELEASKP
                     KFSVVSFWTAVEILLKVPLAHEHWSLVCSPKKPIKKQAYLAGDFQSVTYEETRERLKD
                     VLERPLDRETDSAFDKVRKHRNRVVHFYHPTFTEAEQRQILKEQADAWFALNRLLRDE
                     WKVIFGVKHNWKLAFGETRLIRGNKFYAEVRFKQVKPELEQLSEKNIQIGNCNECHQH
                     ATVTGTETTGNENRKLEVTRCKVCTSAVRQITLVCPDCEIPQLLPEGDSDFECEHCDY
                     TSDRYKLLDEELFHSVDEQLLSVFPAGCTNCMTPESVCKFGDGYLCTQCFIYYTELHV
                     CGCCGHLSDSVPELSHIRGCEFCDGDQRYFDD"
     gene            2430..2864
                     /locus_tag="RH033_002792"
     CDS             2430..2864
                     /locus_tag="RH033_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2991956.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVPGIMGK
                     SGAGKSSLCNALFQGEVTPVSALMTALPGHAAIPLMTRLQDELRTESVRTQTREQFTG
                     AVDRIFDTAESVCVASVVRTALRAVRDTVVSVARAVWNWIFF"
     gene            3082..5478
                     /locus_tag="RH033_002793"
     CDS             3082..5478
                     /locus_tag="RH033_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HDV2991957.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVEEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQTLQQTGDTRELL
                     DILTDDGWLKNEYHGEEEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGTLPVDEAWVDDFIRGAFGCMKKEYVCKDSELATEGATDL
                     WEGSLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENISEYLLLRHQGLQQSIQSL
                     QSHITSLLADIREIADCQEQMTTDVRMAMEEIDTKTRELLTGVCTSLEEELNDYFRSG
                     KRKEQQMLEEEDAEQRRSRSGLWGKISQWSGINNPGWEDYRKRDFDPENPEITLNNRR
                     EAIEYIEEIESTVTSLHREAEAQFRPELEKIVSGIETGFRGTALYATENIAGRINARL
                     EDEGFTVKISFPAVSQLQTRLAEKINLSALMEERTETVTRRRRQSGVWGTVCRWFGTS
                     DLGWENYDEDVSRSVININKVREEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCA
                     FREKVEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML
                     "
     gene            5475..6380
                     /locus_tag="RH033_002794"
     CDS             5475..6380
                     /locus_tag="RH033_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HDV2991958.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTEMTTSLATTNYAITQVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQSETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            6377..7447
                     /locus_tag="RH033_002795"
     CDS             6377..7447
                     /locus_tag="RH033_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDV2991959.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISTINGFDNLMTQPLLSDGPLTVLMDHYLDTDDLADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGTGTTKNSVFQHIQSLPHGQQKEALLASAALPLLFRPREVQG
                     KMYGDGGMGGWQNMQGNTPVTPLVDAGCNMVIVSHLSDGSLWDRRAFPDTTILEIRPR
                     KKLKQTGEEGKSGGLLSFTSAHIDTWRQQGYEDTMLAMEHIRKPLEARQALTRSEAVL
                     QKSLEKTEEADLALRNAMARIK"
     gene            7537..8043
                     /locus_tag="RH033_002796"
     CDS             7537..8043
                     /locus_tag="RH033_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2991960.1"
                     /translation="MVKIDLFNFGEKIMRLSNIFLAVVLTVTVSVAHAIPNMWGSGFT
                     MGVTEFSIDNGKWGINISCTGNPNENNVLAHTIYIYKGDKVVANSEEKNISFVIDGEE
                     FWGVVPDGTRMNDNAWVSFVKAISTATGFELYINEKKVATFNPSAKNVKKVMDNNFIQ
                     SCWNTWYE"
     gene            8099..8728
                     /locus_tag="RH033_002797"
     CDS             8099..8728
                     /locus_tag="RH033_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2991961.1"
                     /translation="MKHFLILVIGLLISITATAESLGQFEEDITGFPQGVWFYKEKAG
                     WKNETGYKKEYLNSLLYKAEEIANYPGNRYTIYRSKNSSIIFVADNETRTFYLLNNKD
                     ALWSDIQIGYWAENKNSCHYVFVDTQYLAQSNELDEFFRTGVLDTHGVGESDRKVKEI
                     RQKHKTHVLLRLPAQCVNDAILSRMNKSERRDLMKAQEDFKQYLARMEL"
     gene            9163..9615
                     /locus_tag="RH033_002798"
     CDS             9163..9615
                     /locus_tag="RH033_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2991962.1"
                     /translation="MKHRHILTFFVAFLFMTIVPDSDAALVKRPEKGRAPFPEYVEFA
                     QNRNQFPCNARLRFKGLCFVEQTDPWGAASVVRIPFNSPNGKGRLNAYGVVVHAIMVG
                     DVVVGGSYSTGKNVTWNFDTFYREGKRRGKVTFYVDGKYWHYRDYPLE"
     gene            9733..9966
                     /locus_tag="RH033_002799"
     CDS             9733..9966
                     /locus_tag="RH033_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HDV2991963.1"
                     /translation="MRGCTLLLFSEAPFLREQEVAVITAYRNVFIQDDPGMHFRWVIR
                     NAEGQRRWRCRNSEPDAGKVLNTRLASDGPLRQ"
     gene            10066..10887
                     /locus_tag="RH033_002800"
     CDS             10066..10887
                     /locus_tag="RH033_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDV2991964.1"
                     /translation="MRLASCFGYAANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQN
                     YTYIPTITVLESLQREGFQPFFACQTCVRDPGRRGYTKHMLRLRRNGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVF
                     DRIEEMRDAMQSLILPPPARQALAQAALTYRYGDEHRPVTTADILTPRRREDYGKDLW
                     STYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESQR"
     gene            10887..11132
                     /locus_tag="RH033_002801"
     CDS             10887..11132
                     /locus_tag="RH033_002801"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2991965.1"
                     /translation="MQHVPEGVIQAPESPDAPEALPGCRSISDDDLCAWCTRLLYRPG
                     EISLCRLSTGNGIWPSVCDRNGYAYGCPEFQPNIIHP"
     gene            11226..11699
                     /locus_tag="RH033_002802"
     CDS             11226..11699
                     /locus_tag="RH033_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDV2991966.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCENYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RVEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            11715..12191
                     /locus_tag="RH033_002803"
     CDS             11715..12191
                     /locus_tag="RH033_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HDV2991967.1"
                     /translation="MQQLSFLPGEMTTRERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            12254..12475
                     /locus_tag="RH033_002804"
     CDS             12254..12475
                     /locus_tag="RH033_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HDV2991968.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSTETYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            12494..13138
                     /locus_tag="RH033_002805"
     CDS             12494..13138
                     /locus_tag="RH033_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HDV2991969.1"
                     /translation="MSRIITTTVYTLHELSSTAQEKARDWYRQHHADSNWYENVYEDF
                     REVCGIFGIDLRQRVFRLSNGRFMEEPCIWFSGFCSQGDGACFEGRWHWQPATPRKIR
                     EYAPQERELHRIADALQAVQKRNFWQLQAEISHRGRYCHPYSMDITVTRNSPTGQALT
                     ADAEAAVSEALRDLAFWLYRQLENEYDWLTSDAAVDEAIHINAYTFTEAGLHAG"
     gene            13188..13562
                     /locus_tag="RH033_002806"
     CDS             13188..13562
                     /locus_tag="RH033_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HDV2991970.1"
                     /translation="MSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYL
                     ADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTIGELNPRHQHTVTLYARGLTCEAD
                     TLGSCGYVYMAVYPTLAPATTS"
     gene            13609..13986
                     /locus_tag="RH033_002807"
     CDS             13609..13986
                     /locus_tag="RH033_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDV2991971.1"
                     /translation="MKTLPDTHVREASRCPSPVTIWQTLLTQLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRSNYRMVNDIIRGEHSEAKR"
     gene            13983..14475
                     /locus_tag="RH033_002808"
                     /pseudo
     CDS             13983..14475
                     /locus_tag="RH033_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14554..15542)
                     /locus_tag="RH033_002809"
                     /pseudo
     CDS             complement(14554..15542)
                     /locus_tag="RH033_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(15690..17283)
                     /locus_tag="RH033_002810"
                     /pseudo
     CDS             complement(15690..17283)
                     /locus_tag="RH033_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(17314..17664)
                     /gene="tnpB"
                     /locus_tag="RH033_002811"
     CDS             complement(17314..17664)
                     /gene="tnpB"
                     /locus_tag="RH033_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDV2991972.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLDETPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTFRLNSLTML"
     gene            complement(17661..17952)
                     /locus_tag="RH033_002812"
                     /pseudo
     CDS             complement(17661..17952)
                     /locus_tag="RH033_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            18270..19127
                     /locus_tag="RH033_002813"
                     /pseudo
     CDS             18270..19127
                     /locus_tag="RH033_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007374439.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(19248..19324)
                     /locus_tag="RH033_002814"
     tRNA            complement(19248..19324)
                     /locus_tag="RH033_002814"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19288..19290),aa:Asp,seq:gtc)
     gene            complement(19457..20188)
                     /gene="dnaQ"
                     /locus_tag="RH033_002815"
     CDS             complement(19457..20188)
                     /gene="dnaQ"
                     /locus_tag="RH033_002815"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HDV2991973.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGETTIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            20253..20720
                     /gene="rnhA"
                     /locus_tag="RH033_002816"
     CDS             20253..20720
                     /gene="rnhA"
                     /locus_tag="RH033_002816"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HDV2991974.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITKWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(20717..21439)
                     /locus_tag="RH033_002817"
     CDS             complement(20717..21439)
                     /locus_tag="RH033_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDV2991975.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            21473..22228
                     /gene="gloB"
                     /locus_tag="RH033_002818"
     CDS             21473..22228
                     /gene="gloB"
                     /locus_tag="RH033_002818"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HDV2991976.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVKKFPQIVVYGPQETQDKGTTQVVKDSETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTPSQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            22300..23658
                     /gene="mltD"
                     /locus_tag="RH033_002819"
     CDS             22300..23658
                     /gene="mltD"
                     /locus_tag="RH033_002819"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HDV2991977.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(23707..24477)
                     /locus_tag="RH033_002820"
     CDS             complement(23707..24477)
                     /locus_tag="RH033_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDV2991978.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     DASVLDMGCGAGHASFVAAQNVSTVVAYDLSAHMLDVVAQAAEARQLKNITTRQGYVE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLTLISEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVKTYFALQNDGSFTSDIIMLEAHKAA"
     gene            complement(24555..25355)
                     /locus_tag="RH033_002821"
     CDS             complement(24555..25355)
                     /locus_tag="RH033_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDV2991979.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            25596..26510
                     /locus_tag="RH033_002822"
     CDS             25596..26510
                     /locus_tag="RH033_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2991980.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(26507..27310)
                     /gene="dkgB"
                     /locus_tag="RH033_002823"
     CDS             complement(26507..27310)
                     /gene="dkgB"
                     /locus_tag="RH033_002823"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HDV2991981.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELDYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPS
                     DEVSAEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGNALKDKVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(27473..27549)
                     /locus_tag="RH033_002824"
     tRNA            complement(27473..27549)
                     /locus_tag="RH033_002824"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27513..27515),aa:Asp,seq:gtc)
BASE COUNT         7033 a         6829 c         7001 g         6730 t
ORIGIN      
        1 tatttaagat taagtcaaaa atttatcgaa agacattcta ttaatcttac atgaatagag
       61 caacgtccgc atctggcaca ggactgctgt tgcagccctg gcaaccttag acttcaatcg
      121 attcagagat ctctaacgca tcatcgactt caatgcccag ataacggact gtgctttcca
      181 gtttcttatg gcctaacaga agttggatca ccctgagatt cttagttttc ttgtaaatca
      241 ggtaaggttt tgttcttctc aaggaatgtg tactgtaaag cgaatcttcg agaccaagtt
      301 ttgctaccca cccatgaaaa attcggttgt attgccgggt tgatatgtgt tggttagtac
      361 cgacccgaga tcggaacaaa tagtctttac tgcgtaaatt gccaagcttt atcaatgcag
      421 aaacagcttc tcttgtccct ttggttatct caaattggac agggctgccg gttttctgtt
      481 gtagaaccgt agctctgctt gaaacagagc taccatatgc aacatcagat actttgagtt
      541 tgactagatc acagcctcga agcttactgt ccaaggccat gttgaacaga gctaaatcgc
      601 gtgttttacc ttccagttca agtcggattc ggatacccca gatatgagat atctgaagtg
      661 gtcttttttg gcctatgata cggtctttgt tccacggtga cgtgttcata ctcaaatctc
      721 ctgcaatgtg ggagatttga gtatggttgt ccttatgagc gaggcgcgga agctcacttt
      781 agggcaatct aattcacatg tcagaggatt tataaaaaga gaatagtgca gattttaggg
      841 atacttacac ttgctatggc atactttttg ggaccccatc agtttaggga tctgaatcgc
      901 cacagctttg gtaggcacct gctttatggt cacgtgcacc cgaagatcct gcgggtacgc
      961 atgggtcacg aaaagtacga gtctacagaa gttaatttac ggttttttgc gctcgatgtg
     1021 gctgccagct accaagtcca gtttacactg gatacaacta acgcgctgta gcaactgcgt
     1081 ggtggaagct gattaaagga gataatgatg ttcgatgttg acgatactgc acccgatact
     1141 cccgcccccc gtgaactcag tcaggatgtg caggcgctga tcaacaatgg tctggatttt
     1201 ctggataaag cccgtgaaga gttggaggcc tcgaagccga agttctccgt cgtcagtttc
     1261 tggactgcgg tcgagatttt actgaaagtt ccgctggcgc acgaacactg gtcgttggtc
     1321 tgcagcccga aaaagccaat aaaaaagcag gcttacctag ccggtgattt tcagtcggtg
     1381 acctacgaag aaacccgtga aaggcttaag gacgtacttg aaagacccct agacagagaa
     1441 acggacagtg cttttgataa ggtcagaaag caccgcaacc gcgtagttca tttctaccac
     1501 cccactttca ccgaggctga acaacgtcaa atactcaaag aacaagctga cgcctggttt
     1561 gccctgaacc ggttattaag ggatgaatgg aaagttatct tcggtgtcaa acataattgg
     1621 aagctggctt tcggtgaaac tcgtctgatt aggggaaaca aattttatgc cgaggtgcgg
     1681 tttaaacagg ttaagcctga actcgagcag ctctctgaaa agaatattca aatcggtaac
     1741 tgcaatgagt gccatcagca tgcgacggta acagggactg aaacgaccgg taatgaaaac
     1801 cgtaagcttg aagttacgcg gtgtaaggtt tgcaccagtg ctgtaaggca aattacccta
     1861 gtttgtccag actgcgaaat acctcaattg ctacctgagg gagatagtga ttttgaatgc
     1921 gaacactgcg actacacttc ggataggtat aaactgctgg atgaggagtt gttccacagt
     1981 gtggatgaac agctactctc cgtatttcct gctggatgta ccaattgcat gactcctgaa
     2041 agcgtatgta aatttggaga cggctatctt tgcactcagt gtttcatcta ttacaccgag
     2101 cttcatgtat gtggttgttg cggtcacctg agcgatagcg ttcctgaact gagccatatc
     2161 cgaggatgtg aattttgtga tggtgaccaa cgttattttg atgactgaat taccatacat
     2221 aatctaggct cttcagggat gggttggaca gtccgctcct ggcacagagc ggactgtcag
     2281 attagattca gacctgtact cattagctga tacaaatcag ccacatatac ctgcattcca
     2341 aataatttca ggcagatatt atctccgtga atccccccgc cacctttccg gtgcgcgggg
     2401 ttttgtcttt tttcaccggg aatacatgta tgaatccgtc tgatgccatt gaggcaattg
     2461 aaaaaccgct ctcctccctg ccttactcac tttcccgtca catcctggaa catctgcgca
     2521 aactcaccag ccacgaaccc gtgcctggca ttatgggtaa aagcggggcc ggtaaatcct
     2581 cactctgtaa tgcactgttt cagggggagg tcaccccggt cagtgcactc atgacagcgc
     2641 ttcccggaca tgccgccatc cctctgatga cacgcctgca ggacgaactg cgcacggagt
     2701 ctgtccgcac tcagacccgt gaacagttta ccggtgcggt ggaccggata tttgacacgg
     2761 cggagagtgt ctgtgttgcc tctgttgtcc gtacggccct gcgcgcagtt cgtgacacgg
     2821 tggtctctgt tgcccgcgcg gtatggaact ggattttctt ctgaacccgg tggtgatgta
     2881 ccaggctgtt gttacagatt tttaactcca tgaaaacact atgcaaacgc agccataccc
     2941 ggggtgtccc cttgcgttgt gcttcccaag ttaatagaat acatcgatcg ttactattat
     3001 atcgatcgtt ataaacgatc gtttgtagta aatgacctcc tccgtggttg tcatgaaaat
     3061 gcgtctcagg aaggttaaat aatgcacgaa aagaacatcg ccctgctttg tgatgaagcc
     3121 gaccgacttt tgcaactgaa cattaatctg ctccggcaaa tggttgagga gccagatgtg
     3181 ttatctgaca gtaagaacga aaacagactg ctttttgata aacagaaagc actgaaaaga
     3241 attgaggagc tggagggcga acaaatcaaa accgcccgca gggagatggt gctggctgtt
     3301 gtcggcacga tgaaagcagg caaatcaacc accatcaacg ccattgtggg gcaggaaatt
     3361 ctgcctaacc gtaaccgccc catgacctct gtaccgacgc tcatccgcca cgttcccgga
     3421 aaaactgagc cggttctcca tctggaacat attcagcctg tccgcaattt attaatcaca
     3481 ctgcaggaaa aactcgccac cccggcagga cagcaggtcg cacagaccct gcagcaaacc
     3541 ggggataccc gcgaactgct ggatattctg acggatgatg gctggctcaa aaatgaatac
     3601 cacggggagg aggaaatctt taccggactg gcatcgttaa acgatctggt tcgtcttgct
     3661 gcggcaatgg ggactgaatt tccttttgat gaatacgcag aagtgcagaa actgccggtg
     3721 atcgacgtgg aattcagcca tctggtgggg atggatgcat gccagggaac actcacactg
     3781 ctggataccc ccggccctaa tgaggccgga caaccgcaga tggaagtgat gatgcgggat
     3841 caactgcaga aagcctctgc ggttctggct gtgatggatt acacccagat gaactcaaaa
     3901 gcggatgaag acgtccgtaa agagcttaat gccattgctg acgtatcagc cggccgcctg
     3961 tttgtactgg tcaataaatt tgatgagaaa gaccgcaatg gcgatggggc agatgccgta
     4021 cgccagaaag ttccggcaat gctgaacagc gatgtgctgc ccgcctcccg cgtttatccc
     4081 ggatcctcac gccaggcata cctggctaac cgtgcgcttc atgagttacg gaaaaacgga
     4141 acccttcctg ttgatgaagc ctgggtcgat gattttatca gaggtgcctt cggttgcatg
     4201 aaaaaagaat acgtctgtaa agacagtgaa ctggcaactg aaggggcgac agacctgtgg
     4261 gaaggctcac ttatcgatca actgataacg gaagtcatac agagctcaca ttccagagct
     4321 gcggcactgg cggttgactc tgccgccgca aaactgatgc agaatgcaga aaatatcagt
     4381 gaatacctgt tgttacgcca tcaggggcta cagcaaagca ttcagtcact gcagtcgcat
     4441 atcaccagcc tgcttgcgga tatccgggaa atcgcggact gtcaggagca gatgaccact
     4501 gatgtcagaa tggccatgga ggagatcgat actaaaaccc gggaattact gacgggggtc
     4561 tgcacctcac tggaagagga gctgaatgac tatttcagaa gcggtaaacg caaagaacag
     4621 caaatgttgg aggaagaaga cgcagaacaa cgaaggtccc ggtccggatt atggggaaag
     4681 atttctcaat ggtccggtat caacaacccg ggctgggaag attacaggaa acgagacttt
     4741 gacccggaaa accccgagat aacactaaat aatcgtaggg aagcaattga atatatagag
     4801 gaaattgagt caactgtgac cagcctgcac cgtgaggctg aagcacagtt ccggcctgag
     4861 ctggagaaaa tcgtcagcgg gattgaaaca ggttttcgtg gcacggccct gtacgccaca
     4921 gaaaacattg ccggtcgcat caatgcccgc ctggaggatg agggctttac cgtaaaaatc
     4981 agttttccgg cagtcagcca gttacagacc cggctcgcgg aaaaaattaa tctgagtgcg
     5041 cttatggagg aaagaacgga gaccgtcacc cgtcgccgtc ggcagagtgg cgtatgggga
     5101 accgtttgtc gatggtttgg caccagtgac ctgggctggg aaaactatga cgaggatgtg
     5161 agtcgcagcg tgatcaatat caacaaggtc agagaggaag ttatgtcact gacccgggca
     5221 tatttcgggg agctgcaggc atccattgag caggatatta accagcccgt ccgccaggaa
     5281 atcgatgcct ttttctgcgc attcagggag aaagttgaac aactgcgtaa cacgctgatt
     5341 cagagctctg aagatcataa acgcgatcag caggcgcagg aacgacttac cgggcgactt
     5401 caggcattaa atgaaagggt tcctgagctc attactgaca gtaaggcgct gagggaagag
     5461 ctggagacaa tgctgtgact tcaccattta ttcaacaaat tgctgataac cgggtatgtc
     5521 aggtactcac ctgtcttcct gaaaaatttg tggttgattt cgcaaacgga atcgatgttg
     5581 cacaggagca tatccgcacg gccggagagc gcacgttctt ccggcgttta aaagaggggc
     5641 tgaccggcga aggcgctgca cggcagaatg ccatcaatgc gtcgcttgca cagggcgttg
     5701 aggcgtccct gcgctggctg acggagatga caacgtcact tgccaccaca aactacgcga
     5761 ttacccaggt aaacgacagg gtcagttcac tggtcagtga tacagccagg ctggcgcatt
     5821 attccgcaga tacgcgggag cagttactca ccctggccga tcaggttcac cataaactga
     5881 atcatcttga agaaaaactc caccgtgttg accaggttca gcgggcgcag ttacatcttg
     5941 agcagatatt ctcatggtgg agcgccgggc gatatgcgtc attttcccct gccggacgtt
     6001 gttatgtggc acttgaagag cttcgctggg gcgcgtttgg tgatgtgata cgtcagagcg
     6061 aaacaggcca ggttaaccag ctactggata ttctcagaca taaagcatta acgcagatgg
     6121 cacaggagag tggcggtagt gcaaccgtgc gtctgaacac tctggactgg cttggtggtc
     6181 agggccggga gcaggcagat aacgagtggc atgacgcgat taactggctg ggagactggt
     6241 gcagtgaaga gcagcatccg gtgatctggt caaccacgca ggctgcagaa cacttaccgg
     6301 ttcgcatgcc tcgcctctgc tctgcagaac gcctctctga aagtatggtc gatgaaatat
     6361 ttcagaaggg ggctgcatga gtacagaaat gaaaacgggg ctggtgctgt ccggtggcgg
     6421 ggcggtgggc gcttatcagg cgggagtggt taaggcactg gcagagtgtg gtacacagat
     6481 cagcatggtt tcaggggcca gcattggcgc attcaatggt gccattatcg cggcctctcc
     6541 cgatctgtca gaagctgccg tacgcctgga ggcgctctgg gatcatctgg ggaataatca
     6601 ggtgctgtcg gtaaacagat tggtttactt ttcattgctg aaaaaattgt tccaggcaat
     6661 gaacctctgc cagatccccg gacgtgcagg agcactgctt acgacgcttc ttcgccatat
     6721 atcgacaatc aacgggtttg acaatctgat gactcagccg ttgttgtcag atgggcccct
     6781 gacagtgctg atggatcatt atcttgatac tgatgatctg gctgacgggc taccgttgta
     6841 tgtgtcgctg taccccacgg aagggggaat gcaggatatt atcgactgca ttcgtgctga
     6901 actgggtacc ggaactacaa aaaactccgt ttttcagcat atccagagcc tgccccacgg
     6961 gcagcagaaa gaagccttac tggcgtcagc agcactgccc ctgctgttcc gtccccgtga
     7021 ggtacaggga aaaatgtacg gtgatggtgg tatgggcgga tggcaaaata tgcaggggaa
     7081 tacccctgtg acgccactgg tggatgccgg atgcaatatg gtgattgtgt cgcatctgag
     7141 tgacggttct ttatgggatc gccgggcttt tccggacacc acaatccttg aaatccgtcc
     7201 ccggaaaaag ctgaaacaaa caggggaaga aggcaaaagt ggcggtctgc tcagttttac
     7261 atcggcacat atcgacacct ggcgtcagca gggctatgaa gacacgatgc tggcgatgga
     7321 gcatatccgg aaaccactgg aggcgcgtca ggcactgacc cggtcagagg cggtgttgca
     7381 gaaaagtctg gagaaaacgg aagaggcaga tttggcactg agaaacgcga tggcccggat
     7441 taaataaaga cgccccaaag aaacgccacc gcctgtggtg gtgtttttcc tggctggtgt
     7501 tattgaaaat attttcagat atatattacc cggacgatgg ttaagattga tttatttaat
     7561 tttggggaga aaattatgag actgtcaaat attttcctgg ctgtagtttt aactgtgacc
     7621 gtatctgtag cacacgctat tccaaatatg tggggcagtg gcttcacgat gggcgttact
     7681 gagttcagta ttgataacgg taagtggggt atcaatatca gttgcacagg taaccccaac
     7741 gaaaacaatg tgctggcgca cacaatctat atttacaaag gagataaggt ggtcgcaaac
     7801 tcggaagaaa aaaatattag tttcgttatt gacggtgaag aattctgggg tgtggttcct
     7861 gacggtaccc gtatgaacga taacgcctgg gttagctttg taaaagcaat aagcactgca
     7921 accgggtttg aactgtacat aaacgagaag aaagttgcga cgtttaaccc gtctgccaaa
     7981 aacgttaaga aagtgatgga taataacttt atccagtcgt gctggaatac ctggtacgaa
     8041 taacgattac aggagagggg gacttcctgg cccccgattt atttaaggag tgcatgaaat
     8101 gaaacatttt ttaattctgg ttatcggttt actcatttcc atcaccgcga ctgccgaatc
     8161 gctgggtcag tttgaagagg atatcaccgg ttttccgcag ggagtatggt tttacaaaga
     8221 aaaagcaggc tggaaaaacg aaaccgggta taaaaaagaa tatttaaaca gtctcctcta
     8281 taaggccgaa gaaattgcga attatccggg taacagatac acgatttacc gtagtaaaaa
     8341 cagcagcata attttcgtcg ctgacaacga aaccaggact ttctacttgc taaacaacaa
     8401 ggacgcctta tggtctgata ttcagatcgg gtattgggct gagaacaaaa actcgtgtca
     8461 ctacgtcttt gtcgatactc aataccttgc gcaaagtaac gaacttgacg agttctttcg
     8521 aaccggggtg ctcgatactc atggtgttgg tgaatcggat cgtaaagtaa aagagatcag
     8581 acaaaaacat aagactcatg tcttactccg cctgcctgcg caatgtgtga atgatgcgat
     8641 cctcagcaga atgaataagt ccgaacgtcg tgatctaatg aaagcgcaag aagatttcaa
     8701 acaatatctt gccagaatgg agctgtaagt attcaacctt ttcgcaactg tgagtgaaat
     8761 gaaaatcaaa actatacttt atactatcat tcttttctgt gaaattggca tcaccgacac
     8821 ttatgcactc ggtggttcgg atatccgtca ctgcgaacca actgaagaat ctgtaattat
     8881 taacccggac gagtcaccgg tgttaatata tagcttcagt aaacacgaag tgtataaact
     8941 gatgcagggg gatacctgca ctgggctagt tattagcgat aaaaagccaa agcctgtcca
     9001 cgtttaatgg acatcgcttg cgaaccgcca gaagtcatgt ctgccagcat tcatctactg
     9061 gccgcggata attatacgac catggatttc tgccataacg ccgaattaat cggtcgtatt
     9121 ccgtaagccg tggtatcgaa taatttaaga aggattttta tcatgaaaca ccgacacatt
     9181 ttaacgttct ttgttgcttt cctgttcatg actatcgtac ctgatagcga cgccgcatta
     9241 gtaaagcgtc ctgagaaagg tcgtgcaccg ttcccggaat atgtggagtt cgcgcaaaac
     9301 cgtaaccagt tcccatgtaa tgcacgttta cgctttaaag gtctctgctt tgttgaacaa
     9361 actgacccct ggggtgcagc aagcgttgtc cgaatccctt tcaattcccc caatggtaaa
     9421 ggcagactta acgcttatgg cgtggttgtt catgcaataa tggttggtga tgttgtcgtt
     9481 ggtggcagct attcaactgg caagaatgtt acctggaatt tcgatacgtt ttatagggag
     9541 ggtaaacgtc gcggtaaggt gaccttttat gttgatggta aatactggca ctatcgcgac
     9601 tatccactcg aataatccat aacgaacaaa actattaaag gaattatgcg tcttatctgc
     9661 gcattattaa aatcaatagt aaattcgctc ctgccattat ggctggggct ttttattttc
     9721 aggtgaaaac acatgagggg ctgtacatta ctcttatttt ccgaagcccc tttcttacgc
     9781 gaacaggagg ttgctgtcat aacagcttac cgcaatgtgt ttatccagga tgacccggga
     9841 atgcatttcc gttgggttat ccgcaacgcg gaaggtcagc gacgctggcg ctgccggaat
     9901 tctgaacccg atgccggaaa ggtgcttaat acgcggctcg ccagtgacgg tcctctaaga
     9961 caataaacgt cattcatttc acccatcagg ctgcgtcttc tccgggagac gtggcttttt
    10021 catttattac accagtaaat cttaaccacc gataaggagc aaagtatgcg attagccagt
    10081 tgttttggtt atgctgcgaa ccagatacgc cgtgaccgtc cactgacaca tgaagagctg
    10141 atgcaccatg tgccggggat tttcggggaa gagaaacaca cgtcccgaag ccagaactat
    10201 acgtacatcc ccaccatcac cgtactggaa agcctgcagc gggaaggctt tcagccattc
    10261 ttcgcctgcc agacctgtgt gcgcgacccg ggccgccggg gatacacaaa acacatgctg
    10321 cgcctgcgcc gtaacggaga gataaacgga gaacatgtcc ctgaaattat tctgctcaac
    10381 tctcatgacg gtacctccag ctaccagatg ctgccgggtt acttcaggtt cgtctgccag
    10441 aacggatgcg tctgtggtca gtctctgggg gaagtgcgtg ttccgcaccg gggaaatgtg
    10501 gtggacagag tcattgaagg ggcttacgag gtggtgggcg tgtttgaccg gatagaggag
    10561 atgcgtgatg ccatgcagtc gctgattctg ccgccaccgg cacgccaggc gctggcacag
    10621 gcggcactga cttaccgtta tggtgacgaa catcggcccg tcaccaccgc cgacattctg
    10681 acgccacgac gccgggagga ttacggtaag gacctgtgga gtacttatca gaccatccag
    10741 gagaatatgc tgaaaggcgg gatttccggt cgcagtgcaa aaggaaaacg tatccacacc
    10801 cgtgccattc acagcatcga caccgatatt aagctcaacc gcgcattgtg ggtaatggca
    10861 gaaacgctgc tggagagcca gcgctgatgc agcatgtccc tgaaggcgtg attcaggcac
    10921 cagaatcacc cgacgccccg gaggcgctgc cggggtgtcg ttccatttct gatgatgacc
    10981 tgtgcgcctg gtgtacccgt ctgctttacc gtcccggaga aatcagtctg tgcagactga
    11041 gtacaggcaa tggcatctgg ccatccgtct gtgaccggaa cggttacgcg tacggctgtc
    11101 cggaatttca gccgaatatc attcatccct gatattaccg ggtctcacgg tcagtgatga
    11161 acgtgatgtt gccagctccc gacctttact ccctctcatt tattcacatt acaaggattt
    11221 catacatgaa aacattgtct cagaacacca cctcttcggc ctgtgcaccg gagactggcc
    11281 tgcaacaact ggttgccacc atagtccctg atgaacagcg catcagcttc tggccgcagc
    11341 attttggcct cattccacag tgggtgaccc tggagccccg tgtcttcggc tggatggacc
    11401 gtctgtgcga aaactactgc ggtggtatct ggaatctgta caccctgaac aacggcgggg
    11461 catttatggc ccctgaaccg gatgacgatg atgacgaaac atgggtactg ttcaatgcca
    11521 tgaacggtaa ccgcgttgaa atgagtccgg aagccgccgg cattgccgcc tgtctgatga
    11581 cgtacagtca tcatgcctgt cgtactgagt gttatgccat gacggtccat tattaccggc
    11641 tgcgggatta cgccctgcaa catccggaat gcagcgccat tatgcgcatc atcgactgaa
    11701 tggaggcagg gacaatgcag cagctttcct ttctgcccgg agaaatgaca accagagagc
    11761 gcagcctcat tcagcgagcc ctgaaaaccc tggaccgcca tcttcatgaa cccggcgtgg
    11821 ccttcacctc cacccgtgcg gcacgggaat ggctgattct gaacatggcg ggactggagc
    11881 gtgaagagtt ccgggtgctg tatctgaata accagaatca gctgattgcc ggtgaaaccc
    11941 tcttcaccgg caccatcaac cgcacggaag tccatccccg ggaagtgatt aaacgcgccc
    12001 tgtatcacaa tgccgcagcc gtggtgctgg cacataatca cccgtccggt gaagtcacac
    12061 ccagcaaggc agaccggctt atcacggaac gtctggtaca ggcactgggc ctggtggata
    12121 tccgggtgcc ggaccatctg atagtcggtg gcagccaggt tttctccttt gccgaacatg
    12181 gtctgcttta acccgtcaca accacatcac acctgttttc acttttatct tctgtcttca
    12241 gaggtatccc attatgaaaa tcatcacccg tggtgaagcc atgcgtattc accaacagca
    12301 tcctgcatcc cgtctttttc cgttctgtac cggcaaatac cgctggcacg gcagcactga
    12361 aacgtatacc ggtcgtgaag tgcaggatat tcccggtgtg ctggccgtgt ttgctgaacg
    12421 ccgtaaggac agttttggcc cgtatgtccg gctgatgagc gtcactctga actgagtcag
    12481 taagggcatt ctgatgagca gaattatcac caccaccgtg tatacactgc atgagctatc
    12541 ctccaccgca caggaaaaag cccgcgactg gtaccgtcag catcatgctg acagcaactg
    12601 gtatgaaaac gtgtatgagg acttcaggga ggtctgtggc attttcggaa ttgacctccg
    12661 ccagcgcgtt ttccggctca gcaacggcag gtttatggaa gagccgtgca tctggttcag
    12721 cggattctgc agccaggggg acggagcctg ttttgaaggg cgctggcact ggcagccagc
    12781 gacaccccgg aaaatcaggg aatatgcccc tcaggagcgt gagcttcatc gtatcgcgga
    12841 tgccctgcag gcggtacaga agcgtaactt ctggcagtta caggctgaaa tcagtcaccg
    12901 gggacgttac tgccatccgt acagcatgga tatcaccgtc acccggaaca gtccgactgg
    12961 tcaggcactg acagccgatg cggaagccgc tgtcagcgaa gccctgcgcg acctggcctt
    13021 ctggctgtac cgccagctgg aaaacgagta cgactggctg acctcagacg ccgctgtaga
    13081 tgaggccatt cacatcaatg catatacctt caccgaagcc ggtctccacg ccggctgacc
    13141 tgtcatcgtt aaactatcat tcaccgattt tctgtgagga ctttatcgtg tcagacacgc
    13201 tccatgaaac aaattatccc gacgacaaca acgaccgccc ctggtggggg ctaccctgca
    13261 ccgtgacgcc ctgtttcggg gcacgtctgg tgcaggaggg caatcagttg cattaccttg
    13321 ccgaccgcgc cggtatcaga gggcagttca gcgacgcgga tgcataccat ctggaccagg
    13381 cttttccact gctgatgaaa caactggaac tcatgctcac catcggtgaa ctgaatcccc
    13441 gccatcagca taccgtcacg ctgtatgcca gagggctgac ctgcgaagcc gacaccctcg
    13501 gcagctgtgg ttacgtttat atggctgttt atccgacact ggcacccgca accacctcat
    13561 aaccccgaat atctcacctt tagcactacc agttacagag aacttaagat gaaaacatta
    13621 cctgacacac acgtacggga ggcatcgcgc tgcccgtctc ccgtcaccat ctggcagaca
    13681 ctgctcaccc aactgttgga ccagcattac ggcctcacac tgaatgacac accgttcgct
    13741 gatgaacgtg tgattgagca gcatattgag gcaggcattt cactgtgcga tgcggtgaac
    13801 tttctcgttg aaaaatacgc actggtacgt accgaccagc caggattcag cgcttgtacc
    13861 cgttctcagt taataaacag tattgatatc ctccgggccc gccgggcaac cggcctgatg
    13921 acccgcagca actacagaat ggtgaacgac atcatccggg gtgaacattc ggaggcgaaa
    13981 cgatgaaact gactctgacg ctcgaagcag acagcgttaa cgtgcaggca ctgaacatgg
    14041 ggcgaattgt cgttgacgtc gatggtattg accccgctga gttgaataat aaggtcgctg
    14101 aaaacggtta ttcactccgc gtggtggatg ctcccgaaca gcagacaacc gactctccac
    14161 caccgtttac tgcccttacc gacatacgtt cgttgcagta ccgcacatat cacggaaaag
    14221 gacaacgcct ggctgtactc actgtcacat cagacgaatg acttcggcga atcagaatgg
    14281 attcatttta caggtaccgg ttatctgtta cgtactgatg cgtggtcgta tccggttctg
    14341 cggcttaaac gtcttggcct gtcaaaaacg ttccgtcgtc tggttgtcac tctcatccgg
    14401 tgttatggcg tcggtctcat tcatctggct gccagtgcag gatgtctgcc gggcctgccc
    14461 actttcgact ggtaactagg aacgacatga catcattaat cacggaaacg gcgctggaaa
    14521 ttctgattgc ctaaatagct gcgcttaata ccgctacact tttgccagcc catgtttgcc
    14581 tccggggaat gggctaacgg tttccataaa atggcgaact tttttcaaca gttgccacat
    14641 tgagcggcac tgatgattac gcgttattgt gtcgtgaagt gcctgccata gccgttcaac
    14701 atgattcacc catggcgagt aaaccggctg ataaatgacc ctgaacttcg ggttctcctt
    14761 cagccagctc tgtgtttccc ggcttttgtg gataatgtag ttgtccacga tcagcgtgat
    14821 ggttttcgcc cgacggtatg tcgctttaag ccgtttcagc aggctgatga acagcgccga
    14881 acttttgctg ttgccgccca catagctggc tttacctgtc ccggcgtgac cacccgtttt
    14941 tgctgtccgc gcagttgcca gtccgcaccg attttgggat taagatggat atccacttca
    15001 tcttcataaa agaccggatg ctctgcgcag cattcgtcca gtgctttatg gattgctgcc
    15061 atcttttcat ctttatgcgg gtcacggata cgcagagttg gcgcagccct tcgccacaca
    15121 atccccgcag acggcaacca gcggcgaacg gttccggcat ttaactggca accggttatc
    15181 tcattgattt ttattgccag cagttctgta ctccagcgtg aacgctggta gccaaagtcg
    15241 ccgggagaat gttttaccag ctcacgtaac agtgtgcaga tatgctcaaa cggccagcga
    15301 cgggcacgcc cggcaggtaa tgatttcagt ccctcaacac ccgactgcgt gaaccagtta
    15361 atccagcgtc caacagagga acgggcgcag cagagcgttc tggcaacgtc gctgacacgg
    15421 tcgccccggt gcagcatcag catggcagtc agtctgcggg cataattttt atcgtgtgtt
    15481 ttatggatgg ctttctgcat caggcgtcgt tcgtcacggg aaattggtgc tatgatcggc
    15541 attgctcagt ccggttggtg atttgttttg atttggcgat tgatcagatc gcacaatccg
    15601 ggctgagttc cctcaaagtg atctactatt ccgcgcagct atttagctgc agaacaatag
    15661 taagcgtcaa cggagcaccg tattgacgct tatttattgg tgagaactac gttccatggc
    15721 aggagttcgt caacacggtt ggagggccat tccggcagta cgctcagaat atggcgcaga
    15781 tacgcttccg gatcgatacc gttcagacgg caggtgccga tcagcccgta cagcagtgct
    15841 ccacgctcgc cgccgtgatc gctaccgaag aacacgtaat ttttctttcc gagacagact
    15901 gcacgaagcg ctctttccgc tgtgttattg tccgcctccg ccagaccgtc atcacggtaa
    15961 taacagaggg cgtcccactg attcagtaca tagctgaacg cttcgcccag tctggatttt
    16021 ttcgacagcg tgccattctt ctccaccatc cattcatgca gcgacgtcag taacgctttg
    16081 cttcgctgct gcctgactgc aagacgctct gactctggta atccccgtat ttcatcctcg
    16141 atggcgtaca gttcactgat tcgcttcagg gcttcttctg ccatcgcact tttgctgctg
    16201 atgtatacat cgtggatttt tctccgggca tgagcacagc acgcaacttc tgtcagcgca
    16261 ccaccttcgc gttctgcact gaacagcctg tcgtaacctg tgaacgcatc cgcctgcagg
    16321 ataccccgga aggggcggag gtgttgctcc gggtgtttcc cctgcctgtt cggtgagtac
    16381 gcgaaccaga ccgccggagg agatgacgaa cccgcattgc gatcatcccg gacatacgtc
    16441 cagatgcgcc ctgttttcgt ctttttcagg cccggtgcca gtacctttac cggtgtgtcg
    16501 tcagtgtgaa gcttgcgggt attcatcaca taacgataca gggcatcatt cagcggcgtc
    16561 attaactggc agcacgcgtc aacccagttg gagagtaatg cacggctcag ttcgacaccc
    16621 tgtcgggcaa agatttcact ctgacgatac agtggcaggt gttcgcagta ttttcccgta
    16681 aacacgcggg caagtaatcc ggggcccgcg ataccacgct ctatcgcggc gggacggcgc
    16741 cggtgcttca acgatgcagt cacattttgt acaggctttt tttacccgtt ctgtgcggat
    16801 cactttcaga gcgctgctca ccagttccag ttgttctgca ctgacttccc ccagataatc
    16861 cagctcaccg ccacactccg ggcaacagct ttcttcagac tccagacggt gtatttcacg
    16921 gggaaggtgt gccgggaacg gacgacgatg gcgcgactgc cgcaactggc ggggaacctg
    16981 tggatcgtct tcccgcccac tgtaacgatc gctgtcctgt tcgcgttgtt tcagcagggc
    17041 ctcagcctgt tcaacttcac gacgcagttt ttcagaacag gtaccgaaca gcatccggcg
    17101 cagtttttct atctgagccc gcagatgttc tatttcccgt tcatcttctt cgatcttttc
    17161 ttcggcacgt gccagtgcag agcgcaggaa ggtcttcgtc tcttcaacca gactcagttg
    17221 ctggtctttc tgacggagct ggctttccag ttctgcaatg cgaatgaggt atttctgact
    17281 catggccgtt tttataatgc ggccaggcat tttttacaac attgtcagtg agttaaggcg
    17341 gaatgttttt ggctgacgcc agtccagctt atcgaggagc attgccagtt gcgagcgggt
    17401 aatggatacc ttaccgtcac gcaccgcagg ccagataaac tggccttcct ccaggcgttt
    17461 ggtgaacagg cacagaccat cagcatcggc ccagagtatt ttaacagtgt caccccgtcg
    17521 gccacgaaag ataaacaggt gacctgagaa gggggtctca tccagcacat gttgtacctg
    17581 ttctcccaga ccgttgaagg atttacgcat atcggtaacg ccggccacga gccagatgcg
    17641 ggtacctgat gggagtgaga tcatcttccc ctcccggtca gttcacggat caacaccgtg
    17701 agcagctctg gtgaaggatt tcccagtgtc attttaccgt aatggaatag ttggaggcat
    17761 tctacgagaa atacgtcctt ctctttgcca gagacgtagc catttaaaaa tcaggttgtt
    17821 atcaacgcca tgttcccggg ccagttgtgc aacattggca tttggtttcg aagccagttc
    17881 aaccatatga agcttaaatt cattagaaaa tatccggcgc gtcccagagc gccagtctgt
    17941 aggtttatcc atcaatagaa ctccgtctaa tttagaagga gctctaattt agtctgcaag
    18001 gatcaggagt tacagacggc attggcttta cgcttacgaa caatatcgac tgcgaatcgg
    18061 gaattatctt tgacaacgat gaggataaaa cagattcggc agtactgttt ccctggattg
    18121 aacgtgcatg tcaggacgtt cgcgagctgt gtcagtttca acgtctgcaa aagacccgta
    18181 cagattaact ccctgtccgt actcattaca cacatacacg tgccagcccc caccggctgg
    18241 cgctttgctt tgtataaagg aaataaataa tgtcagaacc tactctcacc aaccccgaag
    18301 tattaaccga tcataccgat gtgatttgct ccaccagtat tgaacgcatc gtcaccggac
    18361 gtaatgcggc gctgacacag attgaagcat tgctccacca gattaacgat atttcgacac
    18421 aaaccctcag tatcggcgga aaaaccgccc tggactgggt catgaaacag gattttcgct
    18481 gtggttgctg gcttatggag aagcctgaaa cagcgatgaa agccattacc cgcaatctcg
    18541 atcgcggtaa atggcgggat ttaatgaaac gctcagggat gctgtcgatt atggacgctc
    18601 aggcccgtaa ccagtggtac aacagcctag aaaaggatga tattccggtg gtcagcgaag
    18661 cgaatattct gagcaccttt aagcaactgc atttgaacaa gggtgaggtg ttttagcgtg
    18721 gcgtcattaa cgttttcaaa ggattaagct ggaattttaa gacgaattcg ccctgcgggt
    18781 ttggagcgaa aatcattgtg acgggactgg tcaaattcga ccggtgggga ttcgggctta
    18841 accatggctg acagcgtgac cggttagcag accttgagcg aatgctgatg atccttgacg
    18901 ggaagcctgt tcccgacaac cgcgctgacg ttacccgtcg gctgggagac catattcatg
    18961 aaaaccggca cagcaaccac tatgaggatg agatgtttgt gattaagtat tttcagaagg
    19021 gaactgcaca aatcgtattc aagcgacctg agctgattga taagctgaac aacatcatcg
    19081 cccgacacca atccgggagc actgccagcg agatgagagg tgagtgagtt gtctgaagct
    19141 atttcaggtg agccagtgca agccggacgg caatggcata ctggatggca taccgaaaaa
    19201 acaagcatat aaaaggccat gctaatcaac agcatggcct cgaatagtgg cggaacggac
    19261 gggactcgaa cccgcgaccc cctgcgtgac aggcaggtat tctaaccgac tgaactaccg
    19321 ctccgcgttg tgttccgttg ggaacgaggc gaatattacg gattgcctca cccttcgtca
    19381 acggtttttc tcatcttttg aatcgtttgc tgcaaaaatc gcccaagtcg ctatttttag
    19441 cgccttttac aggtatttat gcccgccaga ggcagcttcc gcccttcttc tgcaccagat
    19501 cgagacgggc ttcatgtgct gcaagctctt catctgtcgc aaaaacaacg cgtaacttac
    19561 ttgcctgacg cacaatgcgc tgaattgttg tttcaccttg ttgctgttgt gtctctcctt
    19621 ccatcgcaaa agccatcgac gtttgaccac cggtcatcgc cagataaact tccgcaagga
    19681 tctgggcatc gagtaatgcc ccatgcagcg ttcgtttact gttatctatt tcgtagcggg
    19741 cacataacgc atcgaggctg ttgcgtttac cgggaaacat tttcctcgcc accgcaaggc
    19801 tatcggtgac cttacagaag gtattggtct tcggaatatc gcgcttaagc aacgaaaact
    19861 cgtagtccat aaagccgata tcaaacgctg cgttatggat caccaactcc gcgccgcgga
    19921 tataatccat gaactcatcg gctacctcgg caaacgtggg cttatcgagc aaaaattcat
    19981 cggcaatacc atgtacgcca aaggcttccg gatccaccag ccgatcgggt ttgagataaa
    20041 catggaagtt attgcccgtc aggcgacggt tcaccacttc aacggcacca atctcaatga
    20101 tcttgtggcc ttcatagtgc gcaccaatct ggttcatacc ggtggtttcg gtatcgagaa
    20161 cgatctggcg tgtaattgca gtgctcatag cggtcattta tgtcagactt gtcgttttac
    20221 agttcgattc aattacagga agtctaccag agatgcttaa acaggtagaa attttcaccg
    20281 atggttcgtg tctgggtaat ccaggacctg ggggttacgg cgctatttta cgctatcgcg
    20341 gacgcgagaa aacctttagc gctggctaca cccgcaccac caacaaccgt atggagttga
    20401 tggccgctat tgtcgcgctg gaggcgttaa aagaacattg cgaagtcatt ttgagtaccg
    20461 acagccagta tgtccgccag ggtatcacca agtggatcca taactggaaa aaacgtggct
    20521 ggaaaacagc agacaaaaaa ccagtaaaaa atgtcgatct ctggcaacgt cttgatgctg
    20581 ctctggggca gcatcaaatc aaatgggaat gggttaaagg ccatgccgga cacccggaaa
    20641 acgaacgctg tgatgaactg gctcgtgctg cggcaatgaa tcccacactg gaagatacag
    20701 gctatcaagt cgaagtttaa gcctgtggtt tacgacattg ccgggtggca ccaaccgcct
    20761 ggcgaattcg cggtttattc ttgctctgtt tcatcggatt tagcgttaaa ggaatagtcc
    20821 gtttccgggc aacaataagt tgtaagcaac caagcgcagg aatatgcgca ttcaatagtt
    20881 ttcctccgtg tttgttccac ggaagaacgt ggaaacggct ggcgtgtagc acttcaaaat
    20941 tcaacaaaga gagccagtcc agctgccgca tcagagtgaa catccggctg ttatagggcg
    21001 atgttttgcg caataccggc acaagtttgc gtaatcccat taaactgatg ggattgaagc
    21061 cactaatcac cagccagcca tcatcaatca gtacccgatc ggcttcacgc aataaacgat
    21121 gcggatcagt gcaccacggc aatgtatgtg ccagtagaca aacatcaacg gatttatcgg
    21181 caaaaggaag atgaagtgga tccgcctgca cctggacggg cattccttgc gcagaaacat
    21241 tcacttgatg agaaaccgcg cacgcttcgc aattgatttc tgcgcttaaa ttgccaatct
    21301 taagcagatg aaaaccatac attttagtga accacgggtt gagctgacgc tccagcgcct
    21361 tgcgataaag ctctccccag ggcaaatcgc cccagcaatc aggagccacg acagtttgag
    21421 ggactcttgc cggtttcatc acaaccttcc gtttcacact gagaggtaat ctatgaatct
    21481 taacagtatt cccgcctttg atgacaatta catctgggtt ttgaatgatg aagcaggtcg
    21541 ctgcctgatt gtcgatcccg gagacgcaga gccagtatta aacgccattg ccgccaataa
    21601 ctggcaaccg gaggccatat ttctcaccca ccatcaccac gatcacgttg gcggcgtaaa
    21661 agaactggtg aaaaagtttc cgcaaattgt ggtgtatggt ccacaagaga cacaagataa
    21721 gggaacaaca caggtagtca aagatagcga aactgccttc gttttggggc atgaatttag
    21781 tgtaattgcc acaccgggtc acactttagg acatatctgt tacttcagta aaccttatct
    21841 attttgcggc gacacgctgt tttctggtgg gtgtggtcgg ttgtttgaag ggacaccatc
    21901 acaaatgtat caatcactta aaaagttaag tgcgttacct gacgatacat tggtatgttg
    21961 tgctcatgaa tataccttat caaatatgaa gtttgctttg agtattcttc cgcacgattt
    22021 gtccataaat gattattatc gtaaagttaa ggagttacgg gcaaaaaatc aaataacact
    22081 acccgtaatt ctgaaaaatg agcggcaaat taatgttttt ttaagaacgg aagatattga
    22141 tttaattaat gtaattaatg aagaaacatt attgcaacaa cctgaagaac gttttgcatg
    22201 gttaaggtca aagaaagata ggttctgata aaactttctt gtcatcggct ccgttcgccg
    22261 ttatgatcgg tcgtctttta agcaactatt gacacacaca tgaaggcaaa agcgatatta
    22321 ctcgcctctg tcctgctcgt gggttgccag agtaccggca acgttcaaca gcacgcacag
    22381 agcctttctg cagctggtca aggggaagca gcaaagttta caagtcaggc acgatggatg
    22441 gacgatggga cgtctatcgc gccagatggt gacttgtggg ctttcattgg cgacgagcta
    22501 aagatgggaa ttccggaaaa cgaccggatt cgcgaacaga aacagaaata tttacgcaat
    22561 aagagctatc tccacgatgt aactttacgg gcagagccgt atatgtactg gatagccggg
    22621 caagttaaaa aacgtaacat gcctatggaa ctggtactac tacccatagt ggagagcgct
    22681 tttgatcctc acgcaacgtc tggcgccaat gccgcgggca tctggcagat cattccgagc
    22741 acggggcgca attatggttt gaaacagaca cgcaattatg acgcgcgtcg cgatgttgtt
    22801 gcttcaacaa ctgccgcgct gaacatgatg cagcgtctga acaagatgtt tgacggcgac
    22861 tggcttctga ccgtagcggc ttataacagc ggcgaaggtc gggtcatgaa ggcaattaaa
    22921 acgaacaaag cgcgtgggaa atccacggac ttctggtcgt taccgttgcc gcaggaaacg
    22981 aagcagtacg tgcctaaaat gctggcattg agtgatattc tcaaaaacag caagcgttat
    23041 ggcgtacgtc tgccaacgac cgatgaaagc cgtgctctgg cgcgtgtgca cctgagcagc
    23101 ccggttgaaa tggcgaaggt tgcagatatg gcggggattt ccgtcagcaa gctgaagaca
    23161 ttcaacgcag gcgtgaaagg ctccacgctg ggcgcaagtg gtccgcagta cgtgatggtg
    23221 ccaaagaagc atgcagatca actgcgtgaa tctctggctt caggcgaaat tgctgctgta
    23281 cagtcgacgc tggttgccga caatacgccg cttaacagcc gtgtttacac cgtacgctct
    23341 ggcgacacgc tttcaagtat cgcttcacgt ctcggcgtaa gcaccaaaga tttgcagcag
    23401 tggaacaaac tgcgcggatc taagctgaag ccaggccaaa gtttgacgat tggtgcaggc
    23461 agtagcgcac agcgactggc aaacaacagc gatagcatta cgtatcgtgt gcgcaaaggc
    23521 gattcgcttt caagcattgc taaacgccac ggcgtgaaca tcaaagatgt aatgcgctgg
    23581 aacagcgaca ctgcgaatct gcaaccaggc gataagctga cgttgtttgt gaaaaacaac
    23641 aacatgccag attcctgaca aaccagataa taaaaaaggc accgattccc ccggtgcctt
    23701 ttttatttat gccgctttat gtgcttctag catgatgata tcactggtga aagagccatc
    23761 attctgcaag gcaaaatacg ttttcacctc tgtcgatgcg ctctgttggt aaatgcgaat
    23821 agcgtctact aacgcttctg gcgtacgcat tctcgctacc catgaagaaa attccagcgg
    23881 taacttatct gtaattaaat tatcaactat cagattggct tcactgatta acgtcaacca
    23941 ctcaccgctg gcgtagtttc gtacgtgaga ggtatcgcgt aatgcttcta ccgtctgtaa
    24001 ccagatgtcg cgcactggat gacccggaga cattacgtcc atcacaatca gcctaccgcc
    24061 aggtttcaat atcctattca cttctcgcag tgctgcacca acatcatgcc agtgatgggc
    24121 agaataacgg ctgataacaa tatcaaatgc gttatcggca aatggcaggc tttcgacata
    24181 accctggcgg gtggtgatat ttttcagttg ccgggcttcg gcagcttgtg ccacaacatc
    24241 cagcatatgg gcagataagt catacgccac caccgtgctc acgttttgcg cagcgacaaa
    24301 gctggcatgc cctgctccgc agcccatatc aagtacactt gcatcaggat aatcagccag
    24361 acgcaccgcc agacgctgta aatctcgtcc ggatgcatgt acggtactgg tcagatattc
    24421 gctggcttga gagctaaact gcttttctac gtggtcatgg tgggattgtg ttgtcattgc
    24481 gctgtccttt tgttatttga gtataaaagg gcagcgccaa acgttggtct gccctgtggc
    24541 agacctgaca taccttattt atcaggcttg ccgggactga attcaacgag tagcggattg
    24601 tgatcggacg cgcgcgtaac cagtacagaa gcttcgctga cgttcagacc acggtagaaa
    24661 acaaaatcga gcgggcgacc aaacgcccga cggcgctgat catcggtaaa acgcacctgg
    24721 cgcagcgaca tttcccgtgc aaagcgatat aacgcgttca tccttctacg gctccaggca
    24781 ttgaaatctc ccgccataat gaccgggcca ctgtggtgag ctatctgatc gccaatagga
    24841 agtaactgct tactatagac atccacgccc agactgaagt tgacggcgtg tatattaacc
    24901 accatcaaca ggcgggtgtc aggtaatgga tagaccgtca ccagtgccga cttcgccaga
    24961 cgcaaaatgg gttctcgttc acgtaacggg cagcaataca ctggatgtgc cgccgaaagg
    25021 gtcattacgc cagaaggatg ttgtggcagc acgaaagcgg gtacctgatc ggcggcaaga
    25081 tagttagcag tcgcaaactg tactaactct ggcgttgtct gcgcttcctg caataacacc
    25141 agatgtgcat ctttgccgta gttctttaat accgacaacc attcagcgcg ttgctgtttg
    25201 tatatgttcc acaccaggat ccgaatacgc tcttcggtac ttaacggttc tccaggtggt
    25261 aatgcctggc cgatgctcgc aaaagacccc ggcggtaaga tcctttccgc aggttgtccg
    25321 gcaacatagc gcatggcata ggtgtttttt cgcacttttg actttgaaac ctctatcacg
    25381 tcaacccttc cgcactcggg agggttctac tgttataggg attttagctc actctttcaa
    25441 cgagcaattg tatattgtta tgtaagcaag tgcttacacc ggtcaggatt ttgctcatta
    25501 ttgagaacat taaatcggat ggcatatctc gcatcctgat ggtttaatga caaataaatt
    25561 caaaatatct cttctatttt gcaacaggag caaaaatgaa agccacgtcg gaagaactcg
    25621 ccatttttgt ttcagtcgtc gaaagcggca gctttagccg ggcagcggaa caattagggc
    25681 aagcaaactc agcggtaagc cgggcggtga aaaagctgga gatgaaactt ggcgttagcc
    25741 tgcttaatcg gaccacgcgg caacttagcc tgacggaaga aggcgagcgt tattttcgtc
    25801 gcgtacagtc aattttgcag gagatggcag cggcagaatc agaaattatg gagacgcgta
    25861 atacaccgcg tggactgtta cggatcgatg ccgcaactcc agtggtgctg cactttctga
    25921 tgccgttgat taagcctttt cgtgaacgct atccggaagt cactttatcg ctagtctcct
    25981 ccgaaacgat tattaatttg atcgaaagaa aagtggatgt cgcgatacgc gccggtacgt
    26041 taacggattc cagcttacgt gccaggccgt tatttaacag ttatcgaaaa attatcgcct
    26101 cccccgatta tatttcccgc tacgggaagc cagaaacgat cgacgattta aagcaacata
    26161 tttgcctggg attcactgaa cccgcttccc tcaatacctg gccgatagcc tgtagcgatg
    26221 gacaattaca tgaggtgaag tacggtttgt cctccaatag tggggaaaca ctgaaacagc
    26281 tttgcctgag tggcaacggg attgcgtgtt tgtccgacta catgatcgac aaagaaatcg
    26341 ctcgcggaga attggtggag ttaatggcag ataaagtgtt gccagtggaa atgccattca
    26401 gtgcagtcta ttacagcgac cgtgcggtaa gtacgcgcat ccgggctttt atcgatttcc
    26461 ttagcgagca tgtaaaaaca gctcccggag gagctgtcag agaggcttaa tcccattcag
    26521 gagccagacc ttccgggcta accaggcggt cgttgcaatc cagtgcggcg atcgcttttt
    26581 tatcttcggc atcaagctgt aaattttgtg ccttaagatt actctccagg ttttcacgtt
    26641 tagtagaaga aggaattact gagtaacctt cccccatagc ccacgccaga atcacttgag
    26701 ccggagtcgc attgtgttta gctgcaatac gagcaataac cttatctttc agtgcattgc
    26761 catacgccag agtcatatag gaagtaatat ggataccgtg ctgtttagcc caagcgacca
    26821 ctttacggtt ttgcagataa ggagagagtt caatctggtt agtagcgatg ttttcagcgc
    26881 caacagcagc aatcgctttt tccatcaatg ggatcgtgaa gttggaaata ccgatctcac
    26941 gcgtcagccc ttgttttttg gcttccagca gcgcctgcat aaactcttca gcagagactt
    27001 catcgcttgg tgacggccag tggattagcg tcagatcaac ataatcggta cgcaattttt
    27061 gcaggctctc tttcagactc gggatcaatt tgtctttgct gagattttca atccagattt
    27121 tagtggtgat gtagagttca tgacgtggca cgccactttc tgcaatcgcc tgacctactg
    27181 cggcttcgtt atcatagatt tgtgcggtat caattgcgcg ataatcaagt tcaagcgccg
    27241 ttttcacaga tgaaataaca acgtcgtctt tcagacggaa agtgcctaaa ccaaatgcag
    27301 ggatagccat aatatgcctc ttttaatgct attttcgtta actgaggtga ttatgggctt
    27361 cagactgttg tggaaaaatg gcgtaaaacg cagaggattt ttgcgatttt ggcaataata
    27421 gaaatacaaa cgaaaaaaaa cctcgagcgt taactcgagg cttcttaata agtggcggaa
    27481 cggacgggac tcgaacccgc gaccccctgc gtgacaggca ggtattctaa ccgactgaac
    27541 taccgctcca ccgaattctt ttacaaccac cggtttataa ccggcttact gct
//
LOCUS       DAPHCV010000068        27502 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.068, whole genome shotgun sequence.
ACCESSION   DAPHCV010000068 DAPHCV010000000
VERSION     DAPHCV010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.068"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            67..324
                     /gene="bhsA"
                     /locus_tag="RH033_002825"
     CDS             67..324
                     /gene="bhsA"
                     /locus_tag="RH033_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HDV2991982.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            complement(407..1369)
                     /gene="ldtC"
                     /locus_tag="RH033_002826"
     CDS             complement(407..1369)
                     /gene="ldtC"
                     /locus_tag="RH033_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HDV2991983.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(1513..4959)
                     /gene="mfd"
                     /locus_tag="RH033_002827"
     CDS             complement(1513..4959)
                     /gene="mfd"
                     /locus_tag="RH033_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDV2991984.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEEINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLCFQKLPDLAVQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLL
                     GERVARRRQDSRRAINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            complement(5087..6160)
                     /locus_tag="RH033_002828"
     CDS             complement(5087..6160)
                     /locus_tag="RH033_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HDV2991985.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLSTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLMSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATLMTYVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGTN
                     TIAIYTTHRILVEIFSLTLLAPMNAARWSPQVELTLLMVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            6422..7621
                     /gene="lolC"
                     /locus_tag="RH033_002829"
     CDS             6422..7621
                     /gene="lolC"
                     /locus_tag="RH033_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HDV2991986.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            7614..8315
                     /gene="lolD"
                     /locus_tag="RH033_002830"
     CDS             7614..8315
                     /gene="lolD"
                     /locus_tag="RH033_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HDV2991987.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            8315..9559
                     /gene="lolE"
                     /locus_tag="RH033_002831"
     CDS             8315..9559
                     /gene="lolE"
                     /locus_tag="RH033_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HDV2991988.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            9588..10499
                     /gene="nagK"
                     /locus_tag="RH033_002832"
     CDS             9588..10499
                     /gene="nagK"
                     /locus_tag="RH033_002832"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HDV2991989.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADRRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLAERLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            10515..11336
                     /gene="cobB"
                     /locus_tag="RH033_002833"
     CDS             10515..11336
                     /gene="cobB"
                     /locus_tag="RH033_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDV2991990.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQTFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(11462..12250)
                     /locus_tag="RH033_002834"
     CDS             complement(11462..12250)
                     /locus_tag="RH033_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDV2991991.1"
                     /translation="MKKIMILLSLLMFLPLTAISKPLIPIMKTLFTDVTGTVPDAEEI
                     AHKAELFRQQTGVVPFIVVLPDINNEASLRQNGKAMLAHAASSMSNVKGSVLLLFTTR
                     EPRLIMITNGQVESSMDDKHLGLLVENHTLAYLHADLWYQDINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENDPPGSTTTLGLFAWAVAFIVFATFFNYTTRLYYALKFAVAMAV
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLWTSKR"
     gene            complement(12247..12708)
                     /locus_tag="RH033_002835"
     CDS             complement(12247..12708)
                     /locus_tag="RH033_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HDV2991992.1"
                     /translation="MSQDSRVFFRIFFGIGLVLILISVVIFYNQFTYSKDAIHTEGVI
                     VDTVWHSSHSHRTGKNGSWYPVIAFRPTPDYTLIFNSSIGSDFYEDSEGDKVNVYYSP
                     GHPEKAEINNPWVNFFKWGFIGIMGVIFIAVGLLISMPSSKKTRRKRKSRP"
     gene            complement(12766..13812)
                     /gene="potD"
                     /locus_tag="RH033_002836"
     CDS             complement(12766..13812)
                     /gene="potD"
                     /locus_tag="RH033_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HDV2991993.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGSAS
                     SIYEEYYQKLKAGR"
     gene            complement(13809..14603)
                     /gene="potC"
                     /locus_tag="RH033_002837"
     CDS             complement(13809..14603)
                     /gene="potC"
                     /locus_tag="RH033_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HDV2991994.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            complement(14600..15457)
                     /gene="potB"
                     /locus_tag="RH033_002838"
     CDS             complement(14600..15457)
                     /gene="potB"
                     /locus_tag="RH033_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HDV2991995.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLASLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            complement(15441..16577)
                     /gene="potA"
                     /locus_tag="RH033_002839"
     CDS             complement(15441..16577)
                     /gene="potA"
                     /locus_tag="RH033_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HDV2991996.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            16827..18056
                     /gene="pepT"
                     /locus_tag="RH033_002840"
     CDS             16827..18056
                     /gene="pepT"
                     /locus_tag="RH033_002840"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDV2991997.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            complement(18202..19323)
                     /gene="roxA"
                     /locus_tag="RH033_002841"
                     /gene_synonym="ycfD"
     CDS             complement(18202..19323)
                     /gene="roxA"
                     /locus_tag="RH033_002841"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HDV2991998.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(19399..20859)
                     /gene="phoQ"
                     /locus_tag="RH033_002842"
     CDS             complement(19399..20859)
                     /gene="phoQ"
                     /locus_tag="RH033_002842"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HDV2991999.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(20859..21530)
                     /gene="phoP"
                     /locus_tag="RH033_002843"
     CDS             complement(20859..21530)
                     /gene="phoP"
                     /locus_tag="RH033_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HDV2992000.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(21699..23069)
                     /gene="purB"
                     /locus_tag="RH033_002844"
     CDS             complement(21699..23069)
                     /gene="purB"
                     /locus_tag="RH033_002844"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDV2992001.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(23073..23714)
                     /gene="hflD"
                     /locus_tag="RH033_002845"
     CDS             complement(23073..23714)
                     /gene="hflD"
                     /locus_tag="RH033_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HDV2992002.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNGINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(23750..24856)
                     /gene="mnmA"
                     /locus_tag="RH033_002846"
     CDS             complement(23750..24856)
                     /gene="mnmA"
                     /locus_tag="RH033_002846"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDV2992003.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYDGEVCLGGGIIEQRLPLPV"
     gene            complement(24910..25371)
                     /gene="nudJ"
                     /locus_tag="RH033_002847"
     CDS             complement(24910..25371)
                     /gene="nudJ"
                     /locus_tag="RH033_002847"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HDV2992004.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(25381..26034)
                     /gene="rluE"
                     /locus_tag="RH033_002848"
     CDS             complement(25381..26034)
                     /gene="rluE"
                     /locus_tag="RH033_002848"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HDV2992005.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            26206..27456
                     /gene="icd"
                     /locus_tag="RH033_002849"
     CDS             26206..27456
                     /gene="icd"
                     /locus_tag="RH033_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HDV2992006.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
BASE COUNT         6697 a         6994 c         7149 g         6662 t
ORIGIN      
        1 tatcatttag ttatcgatcg ttaagtaatt gcttgcgacg tcattcatct gcataaggcc
       61 actattatga aaaacgtaaa aaccctcatc gctgcggcga ttttaagctc aatgtcattt
      121 gccagctttg cggctgtcga agttcagtca acgccagaag gccaacaaaa agtcggtact
      181 atcagtgcta acgcggggac aaatctggga tcgctggaag agcagctggc gcaaaaagcg
      241 gatgagatgg gcgcaaaatc tttccgtatt acttctgtaa ccggtccgaa taccctccat
      301 ggaacagcag taatttataa ataagcatta accctcatta atgcctgcta ctgctgattt
      361 ttttccccgc gacatgccgt gtcgcgggga tttttttatc cgagctttac agagtttgtg
      421 ggctcacttg atgacggcgg acatccaccg gcatcccgga acggacatcc atcacatgtt
      481 gcatcacttc agcgtcagtt tgtgctgcat ctttaaatga ttgcattgcg ctattcagcg
      541 taattggcag cagctgcgga tcgtcatcaa tcttctctga cagcggctga tgtacttcaa
      601 ccagacgcgc accgtttggt tcggcagaga cttttatcgg agtgttgatg atattcactt
      661 tggtgcctgg ggtgacctgg ctaaagagtg ttttgatatc gtcatcccgc agacgaatac
      721 agccagaact tacccgcatg ccaatgccga aatcggcgtt cgtaccgtgg agcaaataaa
      781 cgccgccata ggccgccagg cgaatcgcat gatggcccat tggattatcc ggtccagccg
      841 gaactaccgc aggcaactca attccctgtg ctttataacg ggcgcggatg tttgccgttg
      901 gcgtccaggt tgggtttgca cgtttgtctg aaacggtggt caccattgtc ggcgtcagcg
      961 tgtcaccacc taactgacca atgccgatgg gatacacggt taccgaattt ttacccggcg
     1021 ggtagtaata gagacgcagt tccgcaatat tgatcacaat gccttcgcgc ggcgcatctg
     1081 gaagtagggt ttgcaacggg atcgttaaca cgctgcccgc gcgcggtacg taaggatcaa
     1141 cgccggggtt agcctgtaac agagcgagaa agccgacgtt atattttttg gcgatggctt
     1201 ccagagaacc accgtcattt tccaccacat gaaatttgtt ttcgccaacc agacgactgc
     1261 ccgctggcgg cagcggccag gtgttggctt ttgccggtag cgccagcgcc acggcagcgg
     1321 cgaacgtaaa aaacgttagc cagcgagaaa aacgcgtttt gatcatcacc aaaaatccat
     1381 aataaatata aggttattgt aataaacaga taagcctgaa ttatggatgg tgacagtgtc
     1441 ggatagtgca gggaagtgca aagaatttgt aaatgttgca gatgggggcg cagaaacgcc
     1501 cccgatttac cattaagcga tcgcgttctc ttccagttca cgcataaact ggcgtaccca
     1561 ttcgatacgc gttttccgct cactcaaatc ctgaataaac ttcaggcgcg tcggaccatc
     1621 gaggcgataa tgctgcggct gtttttgcag caaaccaatc aaccaggccg gattaacgtg
     1681 attcttctcg gcaaattcga tcacgccgcc tttctcatta ccttccagct tcctgatccc
     1741 cagtttctgc gcttgctggc gcagacgggc aatatccagc agggtacgcg ccggatccgg
     1801 cagcaggccg aagcgatcga taagctcgac tttgatctct tccagttcgt tttccgtttt
     1861 ggcgctggca atacgtttgt agaacgacag acgcgtgttc acgtcaggga tgaaatcatc
     1921 gggcaatagc gacggcatcc gcagctcgac ttctgtttgc tgactggtga gatcttccag
     1981 cgacggctcg cgtccggctt tcagcgcatc gacagcgttt tccagcaact ccatatacag
     2041 cgagaaaccg atggtttcca ttgagccgct ttgttcttcg ccaagcagtt cacctgcgcc
     2101 gcggatctcc agatcgtgcg ttgccagcgc aaaacctgcc ccgagatctt ccagcgaggc
     2161 aatcgcttca agacgttttt gcgcatcggt agtcatcgct tttggatgcg gcgtcagcag
     2221 ccatgcatat gcctggtggt gcgaacgccc gacgcgaccg cgtaactggt gcaactgtgc
     2281 cagaccgaag tgatccgcgc gttcaatgat aatggtgttg gcggtaggaa tgtcgatccc
     2341 ggtttcgata atggtggtac aaaccagcac gttgaaacgt tgatgatgga aatcattcat
     2401 cacccgttcc agttcgcgct cacgcatttg cccgtgaccg atggcaatcc gcgcttcagg
     2461 taccagttct gccagccgtt cggcggcttt ctggatgttt tccacatcat tgtagagata
     2521 ataaacctgt cctccgcgca aaatttcacg caggatcgcc tcccggacca ccagactgtc
     2581 atactcacgg acaaaggttt taactgccag acgacgggcg ggcggcgtgg cgataatcga
     2641 caggtcacgc attccgctca ttgccatatt cagcgtacgt gggatcggcg ttgcagtaag
     2701 cgtcagaata tccacgttcg cgcgcatcgc tttaatgcgc tctttatgac gcaccccgaa
     2761 gcggtgttct tcatcgacaa tcagcaggcc taaatcttta aacttgacgt cactttgcag
     2821 cagtttgtgc gtaccgatca gaatatcgat tttcccttcc gctacttccg caaggatttg
     2881 cgtctgctct ttggcgctgc ggaaacggga gatcatttcg atacgtaccg gccagttggc
     2941 gaaacggtcg cggaagttgt cgtaatgctg ctgcgcgaga agggtggttg gcaccagcac
     3001 cgccacctgc ttgtggttat ctactgccag gaaagcggcg cgcatcgcca cttctgtttt
     3061 accaaagcca acatcgccgc acaccagacg atccattgcc agcggctgac acatgtcgct
     3121 aagcaccgca ttaatggcct gcgcctgatc cggcgtggtt tcaaacggga agctgtcgca
     3181 gaacaactga tactgctcgc gatcgtgttt aaacgcgaag ccctctttgg cggcgcgttg
     3241 cgcgtaaata tccagtaatt ctgccgccac atcacgcact ttttccgccg ctttctgccg
     3301 cgcgcgtgac cacgcatcgc cgccaagttt atgcagcggg gcgttttctt ccgcgccacc
     3361 tgcgtaacgg ctaatcagat gcagtgacga caccggaaca tacagtttgg cgtcgttggc
     3421 ataggtgagc atcaggtact cgccagtaat accgcccgct tccagcgtgg tcattccggc
     3481 atagcgcccg acaccgtgct ccagatggac caccggctga ccaatatgca gctccgcgag
     3541 gttacggatc agtgtatcgg ggttgatggc gcggcgagaa tcctgacgac gacgggcaac
     3601 gcgttcaccg agcagatcgc tttcgcaaat cagcgccaga ttacgtaccg tatcgacaaa
     3661 accatgttcg gcagcgccaa tcatcaaata acgcccacgg tcgctggctt catcaagacg
     3721 cataatgcgt tgcggagcaa ttttaattcg cgcgagcagt tcacccagcg cttcacggcg
     3781 accttcactt tctaccgaga acaccaccgg accgtcgaaa gactcgagga acttacgcag
     3841 cgcatccagc ggtgcttttt gttgtgcctg aacggccagg tctggcagtt tctggaaaca
     3901 taaattggca ttcgcggctt ttgtcggtaa atgttcagtt tttagctgca cacgcggcca
     3961 gtttttcagc tctgaaaaga gctcgtctac ccgcagccag agcgattgtg gtggcaacag
     4021 tgggcgcatc ggatcgacgc cgcgattttc aaaacgcgcc agcgtgtcag cctggaaacg
     4081 ttcggcactg ttttccagat cgccagtatt caccaacagc gtattggcag ggaaataact
     4141 gaacagtggc ggcagcggtt cgctgaagaa tagcggctgc cagtactcga tcccggcagg
     4201 taatgtgcct ttactcactt gctgataaat atgttccgga tcgcgcttca cttcgaaggt
     4261 atcgcgccac tggctgcgaa acagttcaat tgccgcttta tcggtcggaa attcgtgcgc
     4321 gggcagcaga ttgatctctt ctacttcctc cagcgtgcgc tggctatcga cgtcaaacac
     4381 tcgcaggctg tcgatttcat catcaaagaa atcaagacga taaggcagct cactgcccat
     4441 cgggaagaga tccagcaacg cgccgcgcgt ggcgtattcg ccgtgctcca ttacctggtc
     4501 aacatggcga taaccggcgc tgtccagttg ggttcgtaat gcatctcgtg acaggcgctg
     4561 accttttttc atcaccagcg catgaccgtg gagaaaactg tgcgggcaaa cgcgctgcat
     4621 aagcgtattc accggaacaa tcagtacgcc acgctgcatg gtcggtagct ggtaaagggt
     4681 ggaaaggcgc gaggagataa tgtcctgatg aggcgaaaaa ctgtcgtagg gaagggtttc
     4741 ccagtccgcc agattcatca ccatctgatc ggtaaactgg ctgatttcat catgcaaacg
     4801 cagagcattt tgcatatctg gtgcaataag taccaccgga ccggcgtgac gttcggcaat
     4861 ttccgctacc agcgtcgcgc aggcagcgcc ggttaactcg cccagcagac gctgctcacc
     4921 cgctttgacg ggcagcgtat aacgatattg ttcaggcata acggttgtca gattctcgtt
     4981 aggatatgcc tcaacatatg ggggcatatc tctgataagc aatgctgttc attatccgta
     5041 atcgacatgg tttagcaaat tgaatcgccc cggctggggc gatggcttag cgggagtaac
     5101 tgacggcagc gggcagtgaa ggcggggaga acaacagatc gctgaatgca cgctgtgaaa
     5161 gttttcttac cagcaagccc gcaacagtac agatgaacaa actgacaaag ggataaacca
     5221 tcaggagtgt cagttcgact tgcggcgacc agcgtgccgc gttcattggc gcaagcagag
     5281 ttaagctgaa tatctctacc agaatgcgat gggtggtgta gatagcaatg gtgttggtgc
     5341 caatcacatt cagcaggctg gtggagcgca taccgaaacg ttgctcgtat tgataaaaca
     5401 gcttcatgat caccacaatc gataccagcg acaacagcag cgagatagta aacaaccagg
     5461 caccgaccgc cagaacggtc agcaaagaag ccatcagcag atggcggcgc aacggcacct
     5521 ctttaacgta ggtcattaat gttgcgccaa accatgcgcc aaggctgtaa taaggcaaat
     5581 tgcggatcac actgttcatt ccccaccacg gcgtgggaac gaaattaacc gccacactca
     5641 tcagtacaaa caaggcgaat agtggcagcg ccagacggct aaaaatttta cataccacga
     5701 aataaacaat taacgcatac agataccaca aactggtgct ggcggtgatc atcccgtgca
     5761 ggaactcacc ggtggaatcg gcatacgcgg cattggaggc attgcttaaa tcgcgctcag
     5821 gtgccagcca ctggttcagc gcacttaacg ccagccactg caccacgccc caaagcgcca
     5881 gcacccagaa gatgttccag atccgtttat cgagacaatt tccccacggt acgctgtcga
     5941 tataacggcg aatcaaatag ccggatataa agaaaaaaac cggcatacga aagggtgcaa
     6001 ggtaaagatt gaaatagatc cagcatttgc tcaggacttc cgataacgga tgctgaaaag
     6061 tgctcagatg cggataaaag gtaatcaccg agtgataaat cactaccaga caaatacata
     6121 atcctttgat ctggttaatc catagctctt tttgtttcat caggacaaca cgctcttttt
     6181 atttaacatg ctgttgatcc tgaggggcca gtcgcgtggt gtaatgcgta tctgtctgta
     6241 tttggttatt tttcagataa agatcaggct acaaggccga tgagaaaatg aggcccttta
     6301 gccgattgat ttgctggcac ttttcagatt agccctgacg atcacttaca gttcagacgt
     6361 ttacccatcc cgctttcgct tatatactcg tgtctttgct aacagcaacc agacggattt
     6421 catgtaccaa cctgtcgctc tatttattgg cctgcgttac atgcgtgggc gtgcagcgga
     6481 tcgcttcggt cgtttcgtct cctggctttc taccatcggc attaccctcg gggtgatggc
     6541 gctggtcaca gtattgtcag tgatgaacgg ctttgagcgc gagctacaaa acaacatcct
     6601 tggcctgatg ccacaggcaa ttctctcttc tgagcatggc tctcttaacc cgcagcaact
     6661 cccggaaacg gcagtcaaac tggacggcgt taatcgcgtc gcacctatta ctaccggtga
     6721 tgtggtactg caaagcgcgc gcagcgtggc ggtcggggtg atgctgggta tcgacccggc
     6781 acaaaaagat ccactaacgc cgtatctggt caatgtgaaa caaactgacc tcgagccggg
     6841 gaaatataat gtcatcctcg gtgaacagct ggcctcacag ttgggcgtca atcgcggtga
     6901 tcaaatccgc gtgatggtgc catctgccag ccagttcacg ccgatggggc gtattccaag
     6961 ccagcgcctg ttcaatgtga ttggcacttt cgccgccaac agtgaagtcg atggctatga
     7021 aatgctggtg aatattgagg atgcctcgcg tctgatgcgt tatccggcag gcaatattac
     7081 cggctggcgt ttgtggctgg atgagccgct gaaagtcgac tcattaagtc aacaaaaact
     7141 gcctgaaggc agcaaatggc aggactggcg tgaccgtaaa ggcgagctgt tccaggccgt
     7201 acgcatggaa aaaaatatga tgggcttgct gctgagcctg attgttgccg tagcggcgtt
     7261 taacattatt acctcgctgg ggctaatggt gatggagaag cagggcgaag tcgcgatcct
     7321 gcaaacgcaa ggcttaactc cgcgacaaat catgatggtc tttatggtgc aaggggccag
     7381 cgccgggatt atcggtgcga tcctcggagc ggcgcttggc gcactgcttg ccagccagtt
     7441 aaataatctg atgccgataa tcggcgtcct gcttgatggc gcggcgctgc cggtcgctat
     7501 cgaaccttta caggtcattg ttattgcgct ggtggcgatg gctatcgcgc tgctgtctac
     7561 gctttaccct tcatggcgcg ctgccgccac acaacccgct gaggctttac gttatgaata
     7621 agatcctgtt gcaatgcgac aacctgtgca aacgctatca ggaaggcagt gtgcaaaccg
     7681 atgtgctgca caatgtcagt ttcagcgtgg gcgaaggtga aatgatggca attgtcggta
     7741 gctctggttc cggtaaaagt accttgctgc acctgttagg cgggctggat acgccaacct
     7801 ccggcgatgt gatctttaac ggtcagccga tgagcaaact gtcgtcggcg gcgaaagcag
     7861 aactgcgcaa ccagaagctg ggctttattt atcagtttca ccacctgctg ccggatttta
     7921 ctgccctgga aaacgtggct atgccgctgc tgattggcaa gaaaaagccc gctgaaatca
     7981 acagccgtgc gctggagatg ttaaaagcgg tggggctgga gcatcgtgcg aatcaccgcc
     8041 catctgaact ttctggcggc gaacgccagc gcgtggcgat tgcccgtgcg ctggtcaata
     8101 acccgcggct ggtgctggcg gatgaaccta ctggtaacct cgatgcacgt aacgccgaca
     8161 gcatcttcca gttgcttgga gaattgaatc gcttgcaggg caccgccttc ctggtggtta
     8221 ctcacgacct gcaactggca aaacgtatga gccgccaact ggagatgcgt gatggtcgtc
     8281 tgacggcgga actgagcctg atgggggcgg agtaatggcg atgcctttat cgttattgat
     8341 tggcctgcgt tttagccgcg gacggcggcg cggcggcatg gtgtcgctga tctccgtcat
     8401 ttctaccatt ggcattgccc ttggcgtggc ggtattgatc gtcggcttaa gcgcgatgaa
     8461 cggctttgaa cgcgaactga ataaccgcat tctggcggtg gtgccgcatg gcgaaataga
     8521 ggcggtggat caaccgtgga ctaactggca ggaagccctg gataacgtgc aaaaagtgcc
     8581 aggtattgcc gccgctgcgc cgtatatcaa tttcaccgga ctggtagaaa gtggtgccaa
     8641 tctgcgcgca atccaggtga agggcgttaa cccgcaacag gaacagcgtc tgagcgcatt
     8701 accctcgttt gttcaggggg atgcctggcg caattttaaa gcgggcgaac agcaaattat
     8761 catcggcaaa ggcgtggcgg atgcgctgaa agtgaagcag ggcgattggg tgtcgattat
     8821 gatccccaac tcgaatcccg agcataaact gatgcagcca aaacgtgtgc gtttgcacat
     8881 tgccggtatt ttgcagttga gtggtcaact cgatcacagt tttgccatga tcccgctggc
     8941 cgatgctcag caatatcttg atatgggttc cagcgtgtca ggtattgccc ttaaaatgac
     9001 ggatgttttc aacgccaata agctggtacg cgatgcgggg gaagtgacca acagctatgt
     9061 ttatattaaa agctggattg gtacttacgg ctatatgtat cgcgatatcc agatgatccg
     9121 cgccattatg tatctggcga tggtactggt gattggtgtg gcctgtttca acatcgtctc
     9181 caccttagtg atggcggtga aagacaagag tggcgatatc gcagtattaa gaacgctggg
     9241 ggcgaaagat ggcttaattc gcgccatctt tgtctggtat ggattgctgg cagggctgtt
     9301 cggcagcctg tgtggtgtga ttatcggcgt agtggtttca ctgcaactta ccccgattat
     9361 tgagcggatt gaaaagctga tcggtcatca gttcctctcc agcgatatct attttattga
     9421 cttcctgcca tcggaattgc actggctgga cgtcttctac gtactggtca cagcattgtt
     9481 gctgagtctt ttggcaagtt ggtatccggc gcggcgcgcc agtaatattg accctgcgcg
     9541 agtccttagc ggccagtaaa ggcagtacat taaaacaagg agcggcaatg tattacgggt
     9601 ttgatattgg tggaacaaaa attgcgctag gcgtgtttga tagcggtcgg cagttgcagt
     9661 gggaaaagcg ggtgccgaca ccgcgtgaca gctatgacgc atttttagat gcagtgtgcg
     9721 agctggtagc cgaagctgac cggcgttttg gctgtaaagg ttctgtcggc atcggtattc
     9781 cgggaatgcc ggaaacagaa gatggtacgc tgtatgccgc caatgtccct gctgccagcg
     9841 gtaaaccgct gcgtgccgac ctgagcgcac gtcttgatcg cgatgtacgc cttgataacg
     9901 atgccaactg ttttgccctt tctgaagcat gggatgatga atttactcaa tatccactgg
     9961 tgatggggtt gattctcggc accggcgttg gcggcgggct gattttcaac ggtaaaccca
    10021 ttaccggtaa aagctatatt accggcgagt ttggccatat gcgtctgccg gttgatgcgt
    10081 taaccatgat ggggctggat ttcccgttac gccgctgcgg ctgtggtcag catggctgca
    10141 ttgaaaatta tctgtctggt cgcggttttg cgtggctgta tcaacactat tatcatcaac
    10201 cgttgcaggc tcctgagatc attgcgcttt atgatcaagg cgatgagcag gcaagggcgc
    10261 acgttgagcg ttatctggat ttattagcgg tttgtctggg aaatatcctg accattgttg
    10321 accctgacct ggtcgtcatt ggtggtggct tatcgaattt cccggcaatc acaacgcaac
    10381 tggcggaaag gttgcctcgt catctcttac ctgtagctcg tgttccgcgc attgaacgcg
    10441 cgcgccacgg tgatgcgggg ggaatgcgtg gtgcggcctt cctacatcta accgattaat
    10501 caacagaggt tgctatgctg tcgcgtcggg gtcatcggtt aagtcgtttt cgtaaaaata
    10561 aacgccgcct gcgcgagcgt ttgcgtcagc gtattttttt cagagataaa gtggtgccgg
    10621 aagcaatgga aaaaccaaga gtactcgtac tgacaggggc tgggatttct gcggaatcag
    10681 gtattcgtac ctttcgcgcc gcagatggcc tgtgggaaga acatcgggtt gaagatgtgg
    10741 caacgccgga aggttttgat cgcgatcctg aactggtgca aacgttttat aacgcccgtc
    10801 gtcgacagtt gcagcagcca gaaattcagc ctaacgctgc acatcttgcg ctggcgaaac
    10861 tgcaagatgc tctcggcgat cgctttttgc tggtgacgca gaatatcgac aatctgcatg
    10921 aacgcgcagg caataccaat gtgattcata tgcatgggga actgctgaaa gtacgttgtt
    10981 cgcaaagtgg ccaggtcctc gactggaccg gagacgttac gccagaagat aaatgccatt
    11041 gttgccagtt cccggcaccc ttgcgcccgc acgtagtgtg gtttggcgaa atgccactcg
    11101 gcatggatga aatttatatg gcgttgtcga tggccgatat tttcattgct attggtactt
    11161 cggggcatgt ttatccggcg gctgggtttg ttcacgaagc gaaactgcat ggcgcgcaca
    11221 ccgtggaact gaatcttgaa ccgagtcagg ttggtaatga atttgccgag aaatattacg
    11281 gtccggcaag ccaggtggtg ccagagtttg ttgaaaagtt gctgaaggga ttataaagcg
    11341 ggaaaaattg cctgatgcgc tacgcttgac aggcctgtga aggggcgaaa ttaattgaat
    11401 ttgcgggttt tgtgggccgg ataaggcgtt cgcgccgcat ccggcaactt tactgcctct
    11461 tttaccgttt actcgtccat agtaacgcgg caataaacgt acagacacca atcagccctg
    11521 cccagagtgg cgatattctg gtaatcgcaa agctgttatc gatatacaaa cataaagcct
    11581 gataacccat attggcaaca gccatcgcca ctgcaaattt taacgcataa taaaggcgcg
    11641 ttgtgtagtt aaaaaatgta gcaaagacga tgaatgcaac tgcccaggca aaaaggccta
    11701 atgtcgtggt actgccgggt ggatcatttt catgctgttg tccgggatgc ggataatatg
    11761 tcagtggtgg cgtcggttgt tttaatatct gtgcttgtaa aacagccaat gcattattga
    11821 tatcctgata ccagagatcc gcatgtaaat aagccagcgt gtgattttct accagaaggc
    11881 cgagatgttt atcgtccata ctgctttcga cctggccgtt ggttatcata attaaccgtg
    11941 gctcgcgggt agtaaagagt aacagaacac ttccttttac attactcatt gaagatgccg
    12001 catgcgccag catcgctttg ccattttgcc tgagactggc ttcattatta atgtcgggta
    12061 aaaccacaat aaacggtaca actccggttt gttggcgaaa aagttcggct ttatgcgcga
    12121 tctcttcagc atctggtaca gtaccggtaa catccgtaaa aagtgtcttc attattggaa
    12181 ttaagggctt actgattgcc gtcagaggca ggaacatcag taaactgagc agtatcatga
    12241 tttttttcat gggcgggact tccgtttcct gcgtgtcttt ttagaggagg gcatactgat
    12301 taataaaccg acggcgataa aaataacgcc cataatgccg ataaaccccc atttaaaaaa
    12361 attaacccac gggttattta tctcagcctt ttcaggatgt cctggagaat aataaacatt
    12421 aactttatcg ccttcgctgt cttcataaaa atcgctgcca atactcgaat tgaaaattag
    12481 cgtgtaatct ggcgtcggac gaaaggcaat gacgggatac cacgaaccat ttttacccgt
    12541 gcgatgagaa tgactggagt gccagacggt atcaacaatg acgccttcag tatgaatcgc
    12601 gtctttgcta taggtgaact ggttataaaa gataacaaca gaaatgagga tcagtaccag
    12661 accgataccg aagaatatac gaaagaaaac tctactgtcc tggctcatta gccacatcct
    12721 tgctaaataa aaaacgggcg gtaataccac cgcccacttg ctgaattaac gtcctgcttt
    12781 cagcttctga taatactctt cataaatgct gctggcggag cctacgtcat tttgccattc
    12841 accattttta atggtttcag catccgggta gagcgtttta tcgttcgcca cttccggact
    12901 taacagctta cgcgccgcaa ggtttggcgt tggataaccg atagtttcag caacctgttt
    12961 tgccacatcc gggcgcagca ggaagttgat caatttcagc gcgccttctt tgtttttggc
    13021 atttgccggg atcgccaggc tgtccatcca gaaaatgccg ccttctttcg gccataccac
    13081 atcaattggc gtacccgcct ggcgtgcaac gaaagcagaa ccgttccaga tcatgccgag
    13141 gttaacttcg ccttccatat acgggttagc ggggttatcg gagttaaacg ccgcgacgtt
    13201 tggcatcagt tttttcagct cgttgtatgc agcttcaatc tctttcggat cggtggtgtt
    13261 accggagtag cccagcttac gcagcgccat ctggaacact tcacgggcat cgtcggtcag
    13321 cagcaggctg cctttgtact caggcttcca cagatcggcc cagctggtga cagatttcgg
    13381 atccaccgca tcaccgttaa cgccaatcgc cgttgcaccc cagatatacg gaatggaata
    13441 gtcgttattc gggtcaaacg gcttgttgag catgtctgga tcgaggttgc tgaaattgct
    13501 taacttcgac ttgtcgatct tctggatcat cccttcttta cgcattttat cgacgtaata
    13561 ggttgaagga accaccaggt cataggcacc gtctttgtac gttttcagct tcgcgtacat
    13621 ggtttcgttc gactcgtaag tcgaatagat aaccttaata ccggtttctt tggtgaactg
    13681 ttcaagcagt cctggcggca cgtactcggt ccagttgtag aaatacagcg tgttgttgtc
    13741 atcggcgtga gcggcgctca tgcccagtgc cagagcaccc gccgcgagca ggtggcgtga
    13801 ccattttttc atttaacgtc ccctgtgtta cctttcgttt tatcacgagc aataagctgg
    13861 ctggcaatta ccatcaccag cgacagcacc agtaatatgg ttgccagcgc gttaacttcc
    13921 ggcgatacgc cgactttgac catcgaataa atttttaacg gcagaatttc ataactcggc
    13981 ccggtgacaa acgaagaaac caccacgtcg tccatcgaca gggtaaagct taacacccag
    14041 cccgccgcta ccgctggcat tgccagaggc agaatgattt ttcgcagaat ggtaaattcg
    14101 ctggcaccga gatctttcgc cgcttccagc attcgcacgt caaaaccttt caggcgcgaa
    14161 tacactgtca ccaccacaaa tggcaggcag aaggtgatat gcgagaacag cagcgaccag
    14221 aagccaagct gaatacccag cagcataaac agcaccagca gcgaaatcgc catcacgata
    14281 tctggtgaca tcatcaccac aaacagcatt ccgctaacga acggcttacc acgaaagcga
    14341 taacggtaca gcgcaactgc cgtcagtgaa ccgataagcg tagcaaacgt cgccgaaaac
    14401 accgccattg ttagtgaatg ctgcgctgcc tgtaacaggc tgtcgttgtt catcagcagg
    14461 ctataccatt tggtggtaaa accctgccag ttgatgccaa agcgcgagct gttaaaggag
    14521 ttcacaatca aaataatgat tgggatatac aggtacgcgt agatagcggt cataaaaccg
    14581 ccgcgaagca gtcgaccgat cattcgagtt ccaccttctt attcagcaga cgagaagcgc
    14641 gccagtaaac cagcaacatc aggcccatta cgatagtcag cgtaatgctg gtggctgcac
    14701 caaacggcca gtcacgaata ttgaggaact gtaccttgat gacgttaccg atcagcaggt
    14761 ttttcgcacc gcccatcagg tcggacacat agaacaggcc catcgctggc agcatcacca
    14821 gcagacatcc ggcaataatt cccggcatcg ttagtgggat aatgatgcgg ataaaggtct
    14881 gcaacttgct ggcaccgaga tcgcgcgccg cctcaagcaa cggcttatcc agtttttcga
    14941 tactggagta cagcggcatc accataaacg gcagcagaat gtaaaccaga ccgataatca
    15001 ccgcactagg cgtgaacatg atgcgaattg gtgtatcgat aacgcccagc cagagcaaaa
    15061 actcgttgag atagcctttg gtgctgagga aaattttcag cccgtaaata cgaatcaatg
    15121 agttggtcca gaacggaaca atcagcagaa acagcagcag cggacgcacc ttgtgtggca
    15181 gcttcgccag aaaccaggca aaaggatagc ccagcaccag gctggcgagg gtggcgatca
    15241 gcgccatatt cagcgagtgc aatagcactt caaaatagag cggatcgagc agacgcgtgt
    15301 agttatccag cgtaaagacc attttgacga aactggcgtc gtcgcgggtc aaaaagctgg
    15361 tgccaatgat catcaggttg ggcagaaaga caaacaacac aagccaaccg acaatagtga
    15421 caatcactac attctggaac ttacttgtgt tcttcatcag ccagtacgac ctcccagctt
    15481 tctacccaat taatggccat tttttggtcg agagagtggt caaagtcagg atcgtcttca
    15541 ttgaagaatt cgctgaccat caccatcttg ccattttcca gctcaacaac cgactccagc
    15601 gtcatgcctt tgtagttacg ctcgcgaacg taaccaatca gcccttcggc gtggttgtcg
    15661 tcgttaatct cttcaacacg taagtcttcc gggcgcagca gaacatgcag tttttgcccc
    15721 ggttcaacgg cgaagttaac gtagatatta cattcgcggc cttcaacgtt ggcgcgtacg
    15781 cgctgctcgt cgagacgttc gatgacagtg gcgttaaaca tattgatttc gccaataaac
    15841 ccggcaacaa acaggttttt cggctcttcg tagatttcac gcggcgtgcc gtcttgctca
    15901 atgcgaccat cgcgcatcac cacgatcctg tccgacatgg taagtgcttc ttcctggtcg
    15961 tgagttacga acacgaatgt aatgccaagc ttacgctgta acgctttcag ctcgttctgc
    16021 atttgcttgc gcagtttgta atccagcgct gagagcgact catccagcaa caacagacga
    16081 ggcttgttaa ccaccgcgcg agcaatggcg acgcgttgct gttgaccacc agagagctga
    16141 tgcggtttgc gttgagcgaa ggtttccagc tgcaccatcc gcagggcttc catcacgcgg
    16201 ggcgtaattt cagcagcggg ggttttttgc atgcgcaacc caaaggccac attttcgaac
    16261 acggtcatgt gggggaaaag tgcgtagctt tggaaaacag tgttcacgta gcggttttcc
    16321 gccggaacgt gggtgatgtc ctcgttatcc agcatgatgc gtccggaatc aacagtttcc
    16381 agacctgcaa tcaggcgaag aacggttgtt ttaccgcagc cagaagggcc aagcagcgtg
    16441 aggaactcgc cattgttgat agtcagatcc agctggggaa tgacctcttt accatcaaag
    16501 catttgcgaa ttcccgccaa ttgcaccagc ggtgaaagcg aactcggttg tttattcaat
    16561 tttttactct gtcccatgta aacgcaacgg atggcttacc gatgcggggt ttgtggttaa
    16621 ccaccttggt gactcttaat gagggcggta attctacggc aaaccgcttg aatcgccaat
    16681 ctttgttgtg aattactggc ttagctttat attcattaag gtaatgctga taaatattcc
    16741 cgcttgcagg gctaaaagtg acctgacgca atatttgtct tttcttgctt cttaataatg
    16801 ttgtcacaaa aagtgagggt gactacatgg ataaactact tgagcgattt ttgaactacg
    16861 tgtctctgga tacccaatca aaggcagggg tgagacaggt tcccagcacg gaaggccaat
    16921 ggaagttatt gcatctgctg aaagagcagc tcgaagagat ggggcttatc aatgtgacct
    16981 taagtgagaa gggcactttg atggcgacgt taccggctaa cgtccctggc gatatcccgg
    17041 cgattggctt tatttctcat gtggatacct caccggattg cagcggcaaa aatgtgaatc
    17101 cgcaaattgt tgaaaactat cgcggtggcg atattgcgct gggtatcggc gatgaagttt
    17161 tatcaccggt tatgttcccg gtgctgcatc agctactggg tcagacgctg attaccaccg
    17221 atggtaaaac cttgttaggt gccgatgaca aagcaggtat tgcagaaatc atgaccgcgc
    17281 tggcggtatt gcaacaaaaa aacattccgc atggtgatat tcgcgtcgcc tttaccccgg
    17341 atgaagaagt gggcaaaggg gcgaagcatt ttgatgttga cgccttcgat gcccgctggg
    17401 cttacaccgt tgatggtggt ggcgtaggcg aactggagtt tgaaaacttc aacgccgcgt
    17461 cggtcaatat caaaattgtc ggtaacaatg ttcacccggg cacggcgaaa ggagtaatgg
    17521 taaatgcgct gtcgctggcg gcacgtattc atgcggaagt tccggcggat gaaagcccgg
    17581 aaatgacaga aggctatgaa ggtttctatc acctggcgag catgaaaggc accgttgaac
    17641 gggccgatat gcactacatc atccgtgatt tcgaccgtaa acagtttgaa gcgcgtaaac
    17701 gtaaaatgat ggagatcgcc aaaaaagtgg gcaaagggtt acatcctgat tgctacattg
    17761 aactggtgat tgaagacagt tactacaata tgcgcgagaa agtggttgag catccgcata
    17821 ttctcgatat cgcccagcag gcgatgcgtg actgcgatat tgaaccggaa ctgaaaccga
    17881 tccgcggcgg taccgacggc gcgcagttgt cgtttatggg attaccgtgc ccgaacctgt
    17941 tcactggcgg ttacaactat catggtaagc atgagtttgt gactctggaa ggtatggaaa
    18001 aagcggtgca ggtgatcgtc cgtattgccg agttgacggc gcaacggaag tcgtgattgc
    18061 ccgatgcgta tgtggactgc caattaccgg gatgaaacct ttatattgac actggtccac
    18121 atacttatcg tgcctacagg cgttcagtgc atgtaggcac gataaggcgc aatcagcgcc
    18181 gcatccggct taaatccaaa cttacccttc gaagaaccag tatccgctat tgaccagcgc
    18241 cgcgagcatc gcgaggaatg acggatcttc cagcgcatcg ccaaaattct ccgcggtcag
    18301 cgcaatgttg ctggcgagcg catccagtgc cggacggtgc ggggaatcga tcttctcacc
    18361 attggcatac acgtcgtcgc caatgcgcaa tacgcgcaga ccccccaggc gcaccagcac
    18421 atcaccttgt ttcagcgcat cgtagatttc atccggctga taaggcggct ctggcggcgc
    18481 gatatccagt tcatgacgtg actgggatat aaactcgcca aaccattgct taaagtgttc
    18541 cggctggttg atcaattcga gcatcatctc acgcagttta tccatctctt gcggcagaac
    18601 gtccgcagga tgagcgcgag gtggaacatc aggatcgctg tagtagttgc cgcccagttc
    18661 acgttgcagc acataatcgg caaatccact aatcagttcc cgcgtatttg gcgcgcgaaa
    18721 acccacggaa tagttcatcg cattttccag cgcgtagcct tcatgcggga atcctggcgg
    18781 aatataaaga atatcgcctg gctccagttc ttcatcaatg atggcttcga acggatcaac
    18841 ctgtaacaga tccgggtgcg ggcagtgctg tttcatttgc agcttttcgc ccactcgcca
    18901 gcgacggcga ccagtaccct gaatgataaa cacgtcgtac tgatcgagat gcgggccgac
    18961 gccgccgcca ggtacggaaa aagagatcat caggtcatca atacgccagt ccggcagttc
    19021 acggaacggt cgcatcagcg cggcggtcgg ctcatgccag tggttcactg cctgcactaa
    19081 caatgaccag ttagtttcac cgagatgatc gtagctttcg aacgggccgt ggctgacctg
    19141 ccatttgcca tcctggtgac tgaccagtcg actgtcgact tcgctttcca tcgccaaacc
    19201 cgccaactcg tctggagaga tcgggtcaat aaaattatta aagccgcgtt ttaacaccac
    19261 cggacgtttc tgccagtgac gttcaagaaa atcgggccag tttagagtga gttggtattc
    19321 catgttaagc atccacaggc tggtatctgc aaccgattat aacggatgct taacgtaatg
    19381 cgtgaagtat ggacatattt attcatcttt cggcgcagaa tgctggcgac caaaaatcac
    19441 ctccatccgc gcaccgccca gcatgctctc tccggcgacg attttaccct catattgctc
    19501 ggtgatttcg cgggctaccg ccagccctac accttgccca gggcgtaaag tatcaacccg
    19561 ttgaccacgg tcgaaaatga cctctcgctt gcttaatgga ataccggggc catcatcctc
    19621 gaccacaata tagagatgct cgtcggtttg ccttgcagaa atttcgacaa actcgaggca
    19681 atatttacag gcattatcca gcacattgcc catcacctcg acaaaatcgt tctgctcacc
    19741 gacaaagctg atctctggcg aaatatcgag agagatattg acccctttgc gttgatacac
    19801 tttgttcagc gccgaggtga gattgtccag cagtggagcg accggatgca gctcgcggct
    19861 gagcaatgtc ccgccgcgca tactggcacg atgcaggtag tagccaattt gctgtgaaat
    19921 gcggctgatt tgctccagca ttaccggttc agcatcactg acgctcatct tttcactacg
    19981 cagagaacgc agcgtacttt gcagcaccgc cagtggcgtt ttcagactat gggtcaggtc
    20041 ggtgagcgtc gtacgatatt tgtcgtaacg ttcgcgttca ctttttaaca atcggttcag
    20101 gtttcgtacc agactggtca gttctcgcgt tgtggctgga ttgagcaatt cgcggttatg
    20161 ttcttccagt tcgcggactt cttttgccag ggcttcgatg gggcgtaaac tccaccaggc
    20221 ggcgacccac agcagcggga tcactaacag cagattggct gagagcacat agataaacca
    20281 gctccagacc atataggaac tttttagctc caccggaatg gtatccacca ccacaatggt
    20341 caactttggc atccgcgatg ttgccgggta gacgtttacc gccaccgagt gggtcatctc
    20401 cgcgtcgtca tcatcttccc gcacttcctg caactgttgc tgtatcgaat gatctccact
    20461 cagcaagagg ctggtatcgt taacatccgc ttcaatttca tgaaaaccat tcgatttcag
    20521 ccagtcaggc tggatcatct tcatcagcca gggcacgtca cgttgcgccc ataaaagctg
    20581 cccgttctca tcataaatta gtgtcatcgt ggggctttgc ttgtcgatat tttcgggtaa
    20641 ctcgacatgc aacttattgt tttcccactt cgcaagggta tagaacagat tgctctcgcc
    20701 acgtaacagc cgaaacgtgg ttttatcgaa actgacgcta taaccgatca gcgcgaccat
    20761 tccgtaggca agcgaaagca ccagtactac cgctgccgtt gccaacagaa aacgtacccg
    20821 cagcgagagc gggaaaaaaa gacgcagtaa ttttttcatc agcgcaattc gaacagatag
    20881 ccctggccgc gaacggtggt aatcacttct tgcggatatt gtgcctgaat ttttttgcgc
    20941 agacgtccca tcagtacatc aatggtatgg ctttcccgca gctccgcatc cggatagagt
    21001 tggagcatta acgaatcttt gctcaccact ttgccattat tgcgtatcaa cgtttccata
    21061 atggtgtatt cgaacgcggt cagtttgatc acttcgtcat taatagataa ttcacggcga
    21121 gagagatcaa cctgaaacgg tggtagcgaa atgacctgtg aagccagacc gctattacgc
    21181 cgcattaatg cctgcattcg cgccatcacc tcttcaatat gaaacggttt agtcacgtaa
    21241 tcatcagcgc cagcacttaa tacttcgact ttgtcctgcc agctttcacg ggcggttaat
    21301 accagaatcg gcagtgaaac atcattgcta cgccagcggc gaatcagtga cagaccgtcc
    21361 tcgtcaggca aaccgagatc gacaatcgca atatccggta aatgttcatt gagataataa
    21421 tcggcttctt tggcatcttc tgcgtcatcg acctgatgac cagcatcctg aatctgaact
    21481 ttaagatggt gacgtaacaa cgcattgtct tcaacaacca gtacgcgcat ttttatttct
    21541 ccctgtctta attattaaaa tagtgtaacg cgattatgtg gttatggggg taaacattaa
    21601 ataaaccagc gggaagggga ggtaaagtga aaaaacaaaa agtggataat cttaataagc
    21661 aggccggaca gcatcgccat ccggcactga tttgaggctt atttcagctc atcaaccatg
    21721 gtgatggcgc gaccaatata gttagccggc gtcatcgctt tcaggcgggc tttctcttct
    21781 tctggcaacg ccagaccgtc gataaactgc ttcatgcctt cggcgtcaac gcgcttaccg
    21841 cgagtcagct ctttcagctt ctcgtacggt ttttcgatgc catagcgacg cataactgtc
    21901 tggattggct cagccagcac ttcccagttg tgatccagtt catccagcag atggtcacgg
    21961 ttcacttcca gtttgctcac ccctttcagg gtggattgat acgcaatcag cgcataaccg
    22021 atacccacgc cgaggttacg cagcacggtg gagtcggtca ggtcacgctg ccagcgggaa
    22081 accggcagtt tgcttgccag atgctgcaat accgcgttgg aaaggcccag attcccttcg
    22141 gagttttcga agtcgatcgg gttaacttta tgcggcatgg tggaagaacc aatctcacca
    22201 gcaatggttt tctgtttgaa gtggttaagg gcgatataac cccagacgtc acggtcaaag
    22261 tcgatcagaa tggtgttgaa gcgcgcaacg caatcaaaca gctcggcaat gtagtcgtgc
    22321 ggttcgatct gggtagtgta cgggttccac tgaataccca gcgaggtgac gaactcttcg
    22381 ctgaactgat gccagtcaac ttccgggtaa gcggcgatgt gggcgttata gttaccgact
    22441 gcgccgttga ttttaccgag gatctccacc tgattaagct ggcggtactg gcgctccata
    22501 cggtaggcga cgttagccat ctctttaccg atggttgacg gcgtggctgg ctgaccgtgg
    22561 gtacgggaca gcagcgggat atcgcgatac tgaacggcga gatctttaat gccatcaatc
    22621 agttgacgcc agtacggcag gatcacttca tcacgcgcgg ttttcagcat caacgcgtgg
    22681 gagaggttat tgatatcttc cgaagtacag gcaaagtgga tgaattcaga aaccgcatgc
    22741 agttccggga tctccgccac tttttctttc aggaaatact caaccgcttt aacgtcgtgg
    22801 ttagtggtac gctcgatggt tttgatgcgt gctgcatctt cttcgctgaa actggcgaca
    22861 attgcatcaa ggtaaccgat tgcgtcggca gcaaaagcag gaacttcctt gatcgctgcg
    22921 tgcgcggcca gtttttgcag ccaacgtact tcaacttgta cacggaattt cagcaaacca
    22981 tattcgctga aaatcccgcg cagcgcgctg actttatcgc cgtagcgtcc atcgacaggg
    23041 gaaacggcgg tcagtgagga taattccata gatcacaact ccggggttaa atgagcaaga
    23101 atttgttttg cctgagtggt caggcgatta cgagaaaaca tcagttgcag acgtccgccg
    23161 ccgacctggt gccagagcac ggcggcgcga atgcctgcca gcagggtagc gcgaactttc
    23221 gcctgcactt gtgggctttg cagtacagca ggggaaccgg tgacctgaat gcgcgggcca
    23281 agcgggctaa tcacatcaac atagatagca gccatcgcgc tcatcagcgt ttcggactgt
    23341 aaatcgaagt gttcgagctg gcgttgcagg ccgttgatcc cgttgcccag agtgtcgagc
    23401 gcacctttcg ctgaggagag tttgcgctca agcaccatca ggctgagtgt gtagcgggtt
    23461 aactcagcgt ttaagccctg gcgactgctg gcattgagca cgccgagcaa ggtttccagc
    23521 ccgacgcgca ggttggcttc gctaccgcca aaaaccgcca gcgtcgagct ggggttcata
    23581 tcaataatac tgttgagtga gacgtgtagc gcatcggcat cacaatgccc ctggtgagcg
    23641 agttgttgca ccaggcgtgc cgactgacaa ataccggcca gagcgagggt gatgtcatag
    23701 taattctttg ccacgttcac tgcttccttg ttttaagtaa agaaaataat cagaccggca
    23761 gcggcagacg ctgctcaata ataccgccac cgaggcacac ttcaccgtca tagaagacgg
    23821 cagactggcc cggcgtcacg gcggcaaccg gctcatcgaa aatcacttca atgcgatcat
    23881 cgtccagcgc cttgacggtg cacggaatgt cggtctggcg atagcgggtt tttaccgtgc
    23941 aacgcatagt gccggtgaat ggttcgcgat cgacccagtg caactgctgg gcaatcagac
    24001 cgacagacat cagacgcggg tgttcatggc cctgcgcaac aatcagaatg ttgttttcga
    24061 cgtctttatc gaccacatac cacggttctt cggtgccttc tttggtgcca ccaataccca
    24121 gacctttacg ctgaccgagg gtgtgataca tcagcccctg gtgttcacca atttcatcgc
    24181 catcgacggt aatgattttg cccggttgcg ccgggagata acggcccagg aactcgcgga
    24241 atttacgctc gccgataaag cagataccgg tagagtcttt tttcttcgcg gtgaccagac
    24301 cgagatcttc agcaatctta cgcacctgcg gtttttccag ttcgccgacc gggaacaggc
    24361 tttgcgcaat ttgctcatgg ctgagcgtat aaaggaagta gctctggtct ttattgctgt
    24421 ccaggccacg cagcaggcgg ctcttgccat cgacatctgc ccgacgtacg taatgaccgg
    24481 tagcgatata atcggcacct aaatcttcgg cggcaaattc gaggaaagct ttaaatttga
    24541 tctctttgtt gcacagaata tccggatttg gcgtgcgacc ggctttatat tcggcaagga
    24601 acagttcgaa gacgttgtcc cagtactcgg cagcaaagtt aacggtgtgc agttcaatgc
    24661 cgagcttgtc gcagacagcc tgggcatcag ccagatccgc tgccgctgtg caatattcct
    24721 caccgtcgtc ttcttcccag ttcttcataa acaggccttc gacctgatat ccctgttgtt
    24781 gcaacagcca ggcagaaacg gaggaatcga caccgccgga catgccgacg attacttttt
    24841 ttgcggtttc agacattgga tcactcacga cattgaactt caaggcggcg tattctatca
    24901 cgcagccttt tagatgacac cctttgtaaa aggccagtta aaatcgccaa tcatctccag
    24961 cggatagcgt tgcccgcttt gataacaacg aatactttcc gccaccagcg gcgaacgcag
    25021 atttgacgcc tgtaaaattt cttcggcgct gacccaacgg cagcagtcga tatcgctgtc
    25081 atgaggctgg gtcgggcata tttgctcaag ctcaatagca aacaggaaac gcaaaaacgg
    25141 cgttttatct ggcgcaatcc actgatgcat acgaataaag tgttgcggct gcgcgctgat
    25201 gccggtttct tcccacagct cacgggcggc ggcttccact aaggtttcat cggcttccag
    25261 atgcccggca ggttggttcc ataacgcttt accattaatc gtctcttcaa cgactaaaaa
    25321 tttgccttct gcgtgcacca cgcaagcaac ggtaacgtgc ggtttaaaca ttttgcatcc
    25381 ttaatctgtc acttctcgcc attcgccatt ggcaagatta tccaaagagt aatcacccat
    25441 cgcatagcga atcagtcgca gcgtggggaa accaacatgg gcggtcatgc ggcgcacctg
    25501 gcgattacgt ccttcatata aggtgatctt cagccagctg gtgggaatac ttttgcgttc
    25561 acgaattggt ggattccgcg gccataacca cgcaggttcg tcaaccagct ccgcgccggc
    25621 gggcagggta gggccatcat ttaaggttac gccattgcgc aaggcttcaa gtgcgtcttg
    25681 tgtgggaata ccttccacct gcacataata gatttttccg gtgcgtttac ccggctgggt
    25741 taaacgcgcc tgcaacgcgc cgttattggt cagtaccagt aacccttcgc tatcgcggtc
    25801 aagacgacct gccgcataaa cgccctgaac cgggatgaat tcttttaatg ttttgcgtcc
    25861 ggcttcatcg gtgaactgcg gaagaacatc gtagggttta ttgaacagga tcacacgcgt
    25921 gggctggttt tcaggtttac gcctggtaga acgttgcgag ctgaatcgct taacctggtg
    25981 atttctaaaa gaagtttttt gcatggtatt ttcagagatt atgaattgcc gcattatagc
    26041 ctaataacgc gcatctttca tgacggcaaa caatagggta gtattgacaa gccaattaca
    26101 aatcattaac aaaaaattgc tctaaagcat ccgtatcgca ggacgcaaac gcatatgcaa
    26161 cgtggtggca gacgagcaaa ccagtagcgc tcgaaggaga ggtgaatgga aagtaaagta
    26221 gttgttccgg cacaaggcaa gaagatcacc ctgcaaaacg gcaaactcaa cgttcctgaa
    26281 aatccgatta tcccttacat tgaaggtgat ggaatcggtg tagatgtaac cccagccatg
    26341 ctgaaagtgg tcgacgctgc agtcgagaaa gcctataaag gcgagcgtaa aatctcctgg
    26401 atggaaattt acaccggtga aaaatccaca caggtttatg gtcaggacgt ctggctgcct
    26461 gctgaaactc ttgatctgat tcgtgaatat cgcgttgcca ttaaaggtcc gctgaccact
    26521 ccggttggtg gcggtattcg ctctctgaac gttgccctgc gccaggaact ggatctctac
    26581 atctgcctgc gtccggtacg ttactatcag ggcactccaa gcccggttaa acaccctgaa
    26641 ctgaccgata tggttatctt ccgtgaaaac tcggaagaca tttatgcggg tatcgaatgg
    26701 aaagctgact ctgccgacgc cgagaaagtg attaaattcc tgcgtgaaga gatgggcgtg
    26761 aagaaaattc gcttcccgga acattgcggt atcggtatca agccgtgttc tgaagaaggc
    26821 accaaacgtc tggtgcgtgc agcgatcgaa tacgcaattg cgaacgatcg tgactctgtg
    26881 accctggtgc acaaaggcaa catcatgaag ttcaccgaag gcgcgtttaa agactggggc
    26941 taccagctgg cgcgtgaaga gtttggcggt gaactgatcg acggcggccc gtggctgaaa
    27001 gttaaaaacc cgaataccgg caaagagatc gtcattaaag atgtgattgc tgatgcattc
    27061 ctgcaacaga tcctgctgcg tccggctgaa tatgatgtta tcgcctgtat gaacctgaac
    27121 ggtgactaca tttctgacgc tctggctgca caggtcggcg gtatcggtat cgccccaggc
    27181 gcaaacatcg gtgacgaatg cgccctgttt gaagccaccc acggtactgc gccgaaatat
    27241 gccggtcagg acaaagttaa ccctggctct attattctct ccgctgagat gatgttacgc
    27301 catatgggct ggactgaagc cgcagacctg attgttaaag gtatggaagg cgcgattaac
    27361 gcgaagaccg taacctatga cttcgagcgt ctgatggaag gcgctaaact gctgaaatgc
    27421 agcgagtttg gtgatgcgat catcaagaac atgtaatctc tacatgtgtt aaatattgaa
    27481 acgggcgtat aacacgcccg tt
//
LOCUS       DAPHCV010000069        27327 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.069, whole genome shotgun sequence.
ACCESSION   DAPHCV010000069 DAPHCV010000000
VERSION     DAPHCV010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.069"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..185
                     /locus_tag="RH033_002850"
     CDS             <1..185
                     /locus_tag="RH033_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992007.1"
                     /translation="SRKYSSAMITLVILFCRRPHCRKISVQQYYQKRVIFYPTTLQRT
                     AFKKCLFLMRDIIISS"
     gene            146..265
                     /locus_tag="RH033_002851"
     CDS             146..265
                     /locus_tag="RH033_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDV2992008.1"
                     /translation="MPLSYAGYHYFIMMSLVSGEHNTLALPIQEQSPGLVPAG"
     gene            300..644
                     /locus_tag="RH033_002852"
                     /pseudo
     CDS             300..644
                     /locus_tag="RH033_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(1010..1276)
                     /gene="minE"
                     /locus_tag="RH033_002853"
     CDS             complement(1010..1276)
                     /gene="minE"
                     /locus_tag="RH033_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HDV2992009.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(1280..2092)
                     /gene="minD"
                     /locus_tag="RH033_002854"
     CDS             complement(1280..2092)
                     /gene="minD"
                     /locus_tag="RH033_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HDV2992010.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2116..2811)
                     /gene="minC"
                     /locus_tag="RH033_002855"
     CDS             complement(2116..2811)
                     /gene="minC"
                     /locus_tag="RH033_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HDV2992011.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3331..3699
                     /locus_tag="RH033_002856"
     CDS             3331..3699
                     /locus_tag="RH033_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992012.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGNDAGGLQSPACGVVCDPYIC
                     VSSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(3802..4203)
                     /locus_tag="RH033_002857"
     CDS             complement(3802..4203)
                     /locus_tag="RH033_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HDV2992013.1"
                     /translation="MKIKGISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            4445..4738
                     /locus_tag="RH033_002858"
     CDS             4445..4738
                     /locus_tag="RH033_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HDV2992014.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDINK"
     gene            4810..5469
                     /locus_tag="RH033_002859"
     CDS             4810..5469
                     /locus_tag="RH033_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDV2992015.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            5561..6007
                     /locus_tag="RH033_002860"
     CDS             5561..6007
                     /locus_tag="RH033_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HDV2992016.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKGL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(6214..6535)
                     /locus_tag="RH033_002861"
                     /pseudo
     CDS             complement(6214..6535)
                     /locus_tag="RH033_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049639.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin E"
     gene            6801..7220
                     /locus_tag="RH033_002862"
     CDS             6801..7220
                     /locus_tag="RH033_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HDV2992017.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPIIISSEDTLDVFGVVIHVVKAMR"
     gene            7220..8488
                     /gene="umuC"
                     /locus_tag="RH033_002863"
     CDS             7220..8488
                     /gene="umuC"
                     /locus_tag="RH033_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HDV2992018.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVHNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANYAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDEVWGIGRRISKKLDAMGIKTVLDLADSDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFAFNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPCYTTRSSDLLRVK"
     gene            complement(8533..9063)
                     /gene="dsbB"
                     /locus_tag="RH033_002864"
     CDS             complement(8533..9063)
                     /gene="dsbB"
                     /locus_tag="RH033_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HDV2992019.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMARFPEWLPLDKWVPQVFVASGDCAERQWEFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(9209..10750)
                     /gene="nhaB"
                     /locus_tag="RH033_002865"
     CDS             complement(9209..10750)
                     /gene="nhaB"
                     /locus_tag="RH033_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HDV2992020.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRNQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            10972..11691
                     /gene="fadR"
                     /locus_tag="RH033_002866"
     CDS             10972..11691
                     /gene="fadR"
                     /locus_tag="RH033_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HDV2992021.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(11743..13275)
                     /locus_tag="RH033_002867"
     CDS             complement(11743..13275)
                     /locus_tag="RH033_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HDV2992022.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            13605..14903
                     /gene="dadA"
                     /locus_tag="RH033_002868"
     CDS             13605..14903
                     /gene="dadA"
                     /locus_tag="RH033_002868"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HDV2992023.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYLLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPFVGRTGFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            14913..15983
                     /gene="dadX"
                     /locus_tag="RH033_002869"
     CDS             14913..15983
                     /gene="dadX"
                     /locus_tag="RH033_002869"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HDV2992024.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(16040..17776)
                     /locus_tag="RH033_002870"
     CDS             complement(16040..17776)
                     /locus_tag="RH033_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HDV2992025.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(17871..18785)
                     /gene="ldcA"
                     /locus_tag="RH033_002871"
     CDS             complement(17871..18785)
                     /gene="ldcA"
                     /locus_tag="RH033_002871"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HDV2992026.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPQIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAQAMLNNTQEGTQLTISGHPVLKM"
     gene            18885..19496
                     /gene="emtA"
                     /locus_tag="RH033_002872"
     CDS             18885..19496
                     /gene="emtA"
                     /locus_tag="RH033_002872"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HDV2992027.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(19498..20232)
                     /locus_tag="RH033_002873"
     CDS             complement(19498..20232)
                     /locus_tag="RH033_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HDV2992028.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRISWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNNAVLKAQHIIITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRITAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     TGLHEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            20433..20687
                     /locus_tag="RH033_002874"
     CDS             20433..20687
                     /locus_tag="RH033_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDV2992029.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(20737..22707)
                     /locus_tag="RH033_002875"
     CDS             complement(20737..22707)
                     /locus_tag="RH033_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDV2992030.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEANLGYRQG
                     WSRSQDNAYDMHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTHDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTKYAYSEGFIEEGRNFWAGIEYTF"
     gene            complement(22733..23587)
                     /locus_tag="RH033_002876"
     CDS             complement(22733..23587)
                     /locus_tag="RH033_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HDV2992031.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFLRQGG
                     CVSGISVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYATPADIKV
                     SLQPAASI"
     gene            complement(23584..24396)
                     /locus_tag="RH033_002877"
     CDS             complement(23584..24396)
                     /locus_tag="RH033_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDV2992032.1"
                     /translation="MLIDDIDFADLYLQQLRLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLTSKIDLQGAQTLFDMGCGPGTVSLALADKLTTIYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRQAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVVELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYRWYQQQDARALAPASRDWALIWWDSVPQETLR"
     gene            complement(24406..25164)
                     /locus_tag="RH033_002878"
     CDS             complement(24406..25164)
                     /locus_tag="RH033_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV2992033.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLAARRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTKKLAALYRVSADQIHHHLSAIKH"
     gene            complement(25161..26141)
                     /locus_tag="RH033_002879"
     CDS             complement(25161..26141)
                     /locus_tag="RH033_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDV2992034.1"
                     /translation="MRTVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVPV
                     QEQIVFWQIRVPRILAALFLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLFLLLRGGRQ"
     gene            complement(26141..27163)
                     /locus_tag="RH033_002880"
     CDS             complement(26141..27163)
                     /locus_tag="RH033_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HDV2992035.1"
                     /translation="MSLTRRRFTQILASTLFLHHLPSFAQSVKFWASRTLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQFPRLGRLTGRASTLSL
                     EGLMALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLEQSAEQLTTLGKTLG
                     EEHRAAEQANLASRFVGEAQAFATSPAANLSFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDIQHYAQLFWHCSLSDADY
                     QKLVAS"
BASE COUNT         6839 a         6704 c         7062 g         6722 t
ORIGIN      
        1 tgagcagaaa atattcatca gcaatgatta cattagtcat tttattttgt cgacggcctc
       61 attgtcgaaa aataagcgta caacagtatt atcagaaaag agtgattttt tatccaacta
      121 cacttcagcg cactgcgttt aaaaaatgcc tctttcttat gcgggatatc attatttcat
      181 catgatgtct ttggtgagcg gtgaacacaa tacacttgcg ctgcctattc aggagcaatc
      241 ccctggtctg gtgcctgcgg gatgatgttg cagcagagct aaggtcactt agacgctact
      301 atcatttgtc caatggcatg gggtcgaaat cagtcgatac ccgcagtata tatcgtgaac
      361 ttggtgcaac gctgagttac aacatgcgtc tggggaacgg catggaagtt gagccgtggc
      421 tgaaggcggc tgtgcgcaaa gaatttgtcg atgataaccg ggtgaaagtg aataatgacg
      481 gtaattttgt caatgatttg tcgggcagac gtggaatata tcaggcaggt attaaagcct
      541 cattcagcag ttcgttaagc gggcatctcg gggtggggta tagccatggt gccggtgtgg
      601 aatccccatg gaacgcggtg gctggtgcga actggtcgtt ctgaccatca acgattaaac
      661 tgcgcttcgg cgcagttttc gtttaccggg tgctaaaagg gaaaattctg cggcaaaaaa
      721 agcccgccag ttacggcggg aaacctcatc ctatgggaga acaatgaata atgaaattgc
      781 ggggttatca tctcccagta tacccatact aacaataagg ttatttactc agccaggtat
      841 aaacaatttg ttctgtgtgg ggggaacggc attaagatgt aaaggggggg aagcaatgag
      901 tagcatcagc aagaatactc gccgctctta tcaaacgtgg atgaggggga tgaaaaactc
      961 aaggcagaga tgactctgcc ttgaagataa atgcgctttt acagcgggct tatttcagct
     1021 cttctgcttc cggtaaggtc acgttcagct caagaataga aatatcgcca tctttttgct
     1081 caagctgtac ggttaccatc tcaggatcaa tttgtacgta tttacaaatg acctcaagaa
     1141 tatctttacg caactgcggc agataatgcg gttctgcatc actgcgacgg cgttcagcaa
     1201 caataatctg cagccgttct tttgcaatgt tggccgtgtt tttcttccgc gatagaaaga
     1261 aatcgagtaa tgccataact tatcctccga acaagcgttt gaggaagcct ttcttctctt
     1321 cttcaatgaa gcggaaagga cgttcttctc ccaacagacg ttctacggta tctgcgtagg
     1381 ctttacccgc atcggcgtta atgtcgagaa tgaccggttc accctggtta gaggcgcgca
     1441 atactgattg atcctcaggg atcacgccga cgagtttgat gcgcaggatc tccagcacat
     1501 cttccatgct cagcatgtca cctctgctta cgcggcctgg gttatagcgc gttaacagca
     1561 ggtgctcttt aataggctct tcgccatttt ctgcgcggcg tgatttcgac gccagaatgc
     1621 ctaaaatacg gtcagagtcg cgtactgagg agacttccgg gttggtggta ataatggctt
     1681 cgtctgcaaa atagagtgcc attagcgcac cggtctcaat ccctgccggg gagtcgcaaa
     1741 caataaattc aaagtccatc gctttcagat catcaagaac tttggcgacc ccttcacggg
     1801 tgagggcatc tttatcgcga gtttgcgatg ccggaagaat atagagattt tcagtacgct
     1861 tatctttaat taacgcctga tttagcgttg catcgccctg aatgacgttg acgaaatcgt
     1921 aaacgacccg acgttcacaa cccataatca ggtcgagatt acgcaggccg atatcaaaat
     1981 ctatcacgac agttttcttt cccttctggg ccaaaccagt ggcgatggcc gcgctggagg
     2041 ttgtcttacc aaccccccct ttgcccgaag taacaacaat aatgcgtgcc atagaaattc
     2101 cttgttaaaa agggatcaat ttaacggttg aacggtcaaa gcgttttcga ctaactgcag
     2161 tcgcgccgct ttgccataaa attctgctgg gatttgatca ctcagccagt attcacctgc
     2221 gatggacacc agttccgcca tcaggttcgt gcaaaatatt tgcgtttccc ggtcaccact
     2281 tgcccctgcc agcgcacgac cgcgcatcat gccatagaca tgaatgttac catcggcaat
     2341 caattcggcc ccagcgctaa cgtggcttgt aacaatcaga tcacattgtg gagcataaat
     2401 acgctgaccg gaacgcaccg gggtatctat taaacgcgtt tttgtgaccg gcgttgtatt
     2461 ttgcgctgga gcctgcggtg cgggagctgg acgcggcgct ttttcctttc cttccgtcag
     2521 gataggcagc cccatttttt caatttcggc tttaagttgc gcatctttgc agccacttac
     2581 gccaataacc cgcaaaccgg ttgccgaaac cgccttatgc atcgctgacc agtttaccgg
     2641 gtcttccagt gcactgacgt tgagtacaac gggggcatgt tttaaaaatg cgggggcctg
     2701 agcgattttg tcttccagcg cctgatggat aaccttaggt tctgcctcat gcagatgaac
     2761 cacagataaa gtgaagctac tgcctttaag ctcgattggc gtgtttgaca tcctggcctt
     2821 actcaattag ctattaatca tcgccagcgc gcgatgatgt tccgaagact ataaggcatg
     2881 ttatagtctg gattatattg aggcaagtca ccctcccatt tattcagagt aaaagtctat
     2941 tctgtgataa atggcgctga ttcatagctt aaaaaatacc cttgtcaatc aacccattgc
     3001 cgtcgtactt ttgattgttc ttatttacgc gtcctttttc cgcaccctaa tcgtgacagc
     3061 atcttcagac ctgtttaaat atccagtatt gaggcaagtg tattttttca ccacgccagg
     3121 ctttcttaac tttccatttt ttgcggcatc attaaagaac agatacccgt tatcttaata
     3181 tccgccacag aattgctcta tcatagaaac cgcagaagac gtctgatact tctgtcaggt
     3241 catcgtaact cgtccaggtc agcggttttg attacggatt gatgatgaaa tgagcataag
     3301 tgttgtacgg ttggattatc aggatgtgct atgaatatga tgcgtatttt ttatatcgga
     3361 ttgtcaggtg tggggatgat gttctcatca atggcgtctg gaaatgacgc tggcggactt
     3421 caatctccgg cgtgcggcgt tgtttgcgac ccgtatattt gtgtgagttc agatggcatt
     3481 tccccagagt taacaaggaa atatctcggc gaaaaagccg ctgaaaactt acaatcatta
     3541 caaggctacg atcccagcga atttacattc gctaacggtg tattttgcga tgttaaagaa
     3601 aaattatgtc gtgatgatcg ttattttggt gtggatggta agcggagtgg aaaaatcaat
     3661 caaaccacca caaagatgtt atttatgtgt cgtgagtgat gtcttgttta cattatgttc
     3721 tattcgtcat taacctcttg agcgacagag aggagtgggg cgttaacgtt tatgacgggg
     3781 agagtccccg tcggttgaca tttacttaat ttgaatatcg acgttatatt ttttagtttt
     3841 gtttttacgg gcgtcgtttc gggtttgcag cactcgcagc tcatatttgc cagaagcagg
     3901 gagtgagtat tgtccgtgac tatcgagttc tggggaatat ctggataaat caacagaatc
     3961 atcgattcct ggcccgaaga ggtaagtatc agcgccttcg ttagagatac tcacgtgcac
     4021 tttctgacct tttttggcat agaaggtata tgtatcgtaa tcgtatccct ttatttcgcc
     4081 ggagtactga gcgctgctat ggccttttct gaactcaaca ttgacattct tacccgcagc
     4141 aaagctcgtg gaggttagca gtgcaagcaa caatacagcc ttactgatgc ccttaatttt
     4201 cattggtagt ctcctgttca gaatgagtac gatgaactgt tataatataa caatccttca
     4261 cgggagaatt ccagtggctt tcctgtgttc ggttaaatcc cggcgcggca tttttgccgg
     4321 ggggattact tataaagggg gcatggactg aacaaaccgt agaaatctac agctggctgg
     4381 cttgtggcgc gggtttcatt gatgttagct tatgccaaaa cctggcatct taaagagtaa
     4441 aagtatgttt tgtgtgattt atcgaagcag caagcgtgac cagacctatt tatatgtcga
     4501 aaaaaaagac gatttttcgc gtgttcctga ggaactgatg aaaggttttg gtcagcctca
     4561 gttagcgatg attcttccgc tggatgggcg gaagaaactg gttaatgccg atattgaaaa
     4621 ggttaaacag gcattaaccg agcaaggtta ctatttgcag ttaccgccac cacccgaaga
     4681 tttgctgaag caacatcttt ccgtcatggg gcaaaaaaca gacgacatta acaaataacc
     4741 gatatccggc ggtggcatta tctttgtcgg cgcgggtttt catatccacg ataaggtgag
     4801 gggaacgtta tgtatcaaca tcacaactgg caaggtgcgc tgctggatta tccggtgagt
     4861 aaagtagtct gtgttggcag taactatgcc aaacatatta aagagatggg cagcgcagtg
     4921 cccgaagagc cagtgctgtt tattaaacca gaaacggcac tgtgcgatct gcggcagcca
     4981 ttggcgatcc catccgattt cggttcagtt catcatgaag tcgaactggc ggtgttgatt
     5041 ggcgcgacgc tgcgtcaggc tacggaagag catgtccgca aagccattgc cggttacggc
     5101 gtggcgctcg atctgacatt gcgtgatgtt cagggaaaaa tgaagaaagc cgggcagccg
     5161 tgggaaaagg ccaaagcgtt tgataactct tgtccgcttt ccgggtttat tcccgcggcg
     5221 gagtttaccg gcgacccgca aaatacaacg ctgggcctga gcgtaaacgg cgagcaacgc
     5281 cagcaaggta cgactgcgga catgatccat aaaatcgttc cgctgatcgc ttatatgagc
     5341 aagtttttta ccctcaaggc cggtgacgtt gtgctgacag gcacgcctga tggtgtcggc
     5401 ccgttgcaaa gcggtgatga gctgacagtc actttcgatg ggcattcttt gacaactcgc
     5461 gttttgtaat actttttgcc gcctgaaagc ggcggcaaaa cttgcatcgc tatgccagac
     5521 tggttataag gtgcgtttta acgtaatggc ggaacacctg atgagcgatg tacctttctg
     5581 gcaaagtaaa accctggacg aaatgagcga tgcggaatgg gagtcgttgt gtgatggttg
     5641 cggtcagtgt tgcctgcata aactgatgga tgaagacacc gacgaaatct acttcactaa
     5701 cgtcgcctgt cgccagctca atattaaaac ctgtcaatgt cggaactacg aacgtcgttt
     5761 cgagtttgaa cccgactgca ttaaattaac ccgtgaaaat ctgccaacat tcgaatggct
     5821 gccaatgacc tgcgcttatc gtttgctggc ggaaggtaaa ggtttacctg cgtggcatcc
     5881 gctgcttact ggttcgaaag cggcaatgca tggtgaacgt atctctgtgc gtcatatcgc
     5941 agtaaaagaa tcagaagtga ttgactggca ggatcatatc ttaaataaac ctgactgggc
     6001 gcagtgataa atttaataga tgttgatggg ggctttctta attcacgtct ggatgctcgc
     6061 catttcgtag ccctgttttt gaaagcgctc tgtcataatg agagttaccc gataccttat
     6121 tatcaacagg taactctcaa tctttaaaaa acgtctgtat ttttaaatgt gataaacttt
     6181 ataccttgag tacatagaga gaataatcgc ttatcagact tcaggtatct caaagagtgt
     6241 cttcttaccg tgtcttttct gatactcatt acaggtgtta atcatttttt tggccgctta
     6301 cttttagtaa agaaagcatt aaatcatcat aatcaacgta gaatctggtt gtttcagttt
     6361 ccgtttttat caccccgatg gcggctattt cggtagttaa tttcaatttg gcggcatcga
     6421 tatctttatt cgcttgttta accgtgttag acagggtggt aaagaaactc tgcacagatt
     6481 ttaacttgtt cttcaattct ggaatcagtt tcccttcaac tacgcccgca gcaatagaac
     6541 acagtttctt gatgccgtca tccagtacct taatgagaat gcctttctgg gcgaatgctt
     6601 cgattctatt ttaaaccagg tgatattttt cctttctgct ggatggacgc tgtcgccaga
     6661 aggccgcgtg agcacaggat aagagaacga aaaatcagca gtctatgcag cgacaaatat
     6721 tgatagcctg aatcagtatt gatctgctga caagaacaga ctactgtata taaaaacagt
     6781 atagcttcag gcagattatt atgttgttta tcaagcctgc ggatctccgc gaaattgtga
     6841 cttttccgct atttagcgat cttgttcagt gtggctttcc ttcaccggcc gcagattacg
     6901 ttgaacagcg catcgatctg aatcaactgt tgatccagca tcccagcgcg acttatttcg
     6961 tcaaagcaag tggtgattct atgattgatg gtgggattag tgacggtgat ttactgattg
     7021 tcgatagcgc tattaccgcc agccatggtg atattgtcat cgctgctgtt gacggtgagt
     7081 ttacggtgaa aaaattgcaa ctacgcccga cggtacagct tattcctatg aacagtgcgt
     7141 actcgcccat tatcatcagt agcgaagata cgctggatgt ctttggtgtg gtgatccacg
     7201 tcgttaaggc gatgcgctga tgtttgccct ctgtgatgta aacgcgtttt atgccagctg
     7261 tgagacggtg tttcgccctg atttatgggg taaaccggtg gttgtgctat cgaataatga
     7321 cggttgcgtt atcgcccgaa acgctgaggc aaaggcgctg ggcgttaaaa tgggcgatcc
     7381 ctggttcaaa caaaaagatc tgtttcgtcg ctgtggcgtg gtttgcttta gcagcaatta
     7441 tgagctttac gcagacatga gtaatcgggt gatgtcgacg ctggaagaac tatcgccccg
     7501 cgtcgagatt tacagtattg atgaggcatt ctgcgatctg acaggtgtgc ataattgtcg
     7561 cgatctgact gattttggca gagaaattcg cgcaacggtg ctacaacgta cccatcttac
     7621 tgttggtgtg gggatcgccc agaccaaaac gctggctaag cttgccaatt atgcggcaaa
     7681 aaaatggcag cggcagacgg gcggggtggt ggatttatca aatctggaac gccagcgtaa
     7741 attaatgtct gctctccccg tggatgaagt ctgggggatt ggacggcgga tcagcaaaaa
     7801 actggacgcg atggggatca aaaccgttct cgatttggcg gattcagata tccggtttat
     7861 ccgtaaacat tttaatgtcg tgctcgaaag aacggtgcgt gaactgcgcg gtgaaccctg
     7921 tttgcaactg gaagagtttg caccgacgaa gcaggaaatt atctgttccc gctcgtttgg
     7981 tgaacgcatc acggattatc cgtcgatgcg gcaggccatt tgtagttacg ctgcccgggc
     8041 ggcggaaaaa cttcgcagcg agcatcaata ttgtcggttt atctccacgt ttattaagac
     8101 gtcaccattt gcgttcaatg aaccttatta cggcaatagc gcatcggtaa aactgctgac
     8161 gcccactcag gacagcaggg atatcattaa cgccgctacg cgatctctgg atgccatctg
     8221 gcaagcgggc catcgctacc aaaaagcggg cgtgatgctg ggggatttct tcagtcaggg
     8281 agtcgcgcag ctcaatttat tcgatgacaa cgcaccgcgc cccgggagtg agcaattgat
     8341 ggcggtaatg gatacgctga atgctaaaga gggcagagga acactctatt ttgccgggca
     8401 ggggatccag caacaatggc agatgaagcg tgccatgctt tcaccatgtt atacaacgcg
     8461 aagttctgat ttactgcggg tcaaataaac atagcggcag gaaaaagcgc tcccgcagga
     8521 gcgccgaatg gattagcgac cgaacaggtc acgttttttc gctttaaacg gctgggaaat
     8581 caccaccagc accgcgacaa tcaggtaagc gataaaaata ccgagcagcc actgcggcat
     8641 ttccagacct aaaaattccc actgacgctc ggcgcaatcg ccagaggcga caaacacttg
     8701 cggcacccat ttatccagcg gcagccattc cgggaaacga gccataaaat cacaggtcgc
     8761 aaacggcgaa ggatagagct gaagcatggt gtgctcgtaa gttaactgca caccgcggaa
     8821 cgcactatac aaccagataa ccatcgctac ataacgcagc ggagttttcg gggcgatcgc
     8881 gccaatcagc gcagcaccca gaacgccgaa taacgcgcag cgttcataaa tacagagcac
     8941 gcaaggtttc agtaacatca catgctggaa ccacagcgcc gtcagttcca gtgccagagc
     9001 agtaaacgcc atcaacagcc atgcgccccg gccttgtgaa cattggttca aaaatcgcaa
     9061 cataatcatt tccctgcaat atgcatagag tgcgcagttt aaaccaattc attcgctgcg
     9121 ccaccaggag gcagcaaaaa taggacgcaa ttggacaatt atcgggcgaa aaggcaaacc
     9181 gggcatttat atgcccggta agttgttatc aaagcgttgc tatccagccc atttgcataa
     9241 accattcggt tacaggagca agcgtaaact cgacgcagag cagcccgacg agtgtcagga
     9301 cgagggtgta aggcagggcc atccacacca tgcggccata agagagacga atcaatggcg
     9361 cgagtgcaga ggtcagcagg aacaggaacg cagcctgacc gttcggcgta gcgacggagg
     9421 gcagattggt accggtatta atggcgaccg ccagcagctc atattgcttc aacgtgatag
     9481 cgccactttc cattgccgct ttcgcttcgt tgatataaat cgtccccacg aagacgttat
     9541 ccgaaatgga tgacagcaga ccgttgaaaa tatagaacaa cgacagctga gcatgttcag
     9601 atgcctgcaa cacaaactga ataatcggcg aaaacagttg ttggtcgata atcaccgcga
     9661 ctactgagaa aaacaccgtc aacagtgcgg tgaatggcag agattcggtg aaggctttac
     9721 cgatagcatg ttcatcggta acaccggtca atgatgtcgc cagaataatg accgacaaac
     9781 caatcaaacc gacttctgcc agatgcagtg ccagcgcagt caccagccag acgccgataa
     9841 tcgcctggac aatcagacgg attttatcct gacgggtacg ctggttgcgg ctttgatcgt
     9901 caaactgttg cagcacttcg cggactttct ccggcagcgt ttcaccgtag ccaaaccaac
     9961 gcagcttctc taccagcagg caggttaaca ggccacaaat cagaaccggg acggtcaccg
    10021 gcgacatgcg caggaagaaa tcgccaaaat gccagccagc cgctttagcg atgatcaggt
    10081 tttgtggttc gcccaccatg gtcatcacgc cgcctaatgc ggtgccgaca ccggcatgca
    10141 tcatcagact acgcagaaag ccacggaact gttccagaac cactttgtaa tgcttgtcga
    10201 tatgactatc gtcttgcagg tcggtatctt cagtacggga agaggctacg cggtgataaa
    10261 taccataaaa accgactgca acgctgatca ccaccgccac gacggttaag gcatcgagga
    10321 acgcggagag gaacgccgcc gccacgcaaa aagagagcga cagcagcatt ttggagcgaa
    10381 tacttaacag caaacgggta aatatgaaca gcaacagctg tttcataaaa tagatacccg
    10441 ccaccataaa catcagtaac agcaagactt caagatttgc cgccacctct tcacggacgt
    10501 gttccgcgct ggtcatgccg atgaatactg cttcgatagc caacaaacca ccggggagca
    10561 gcgggtagca tttcagggcc atcgccagag tgaaaataaa ttccgctacc agcaaccagc
    10621 cagcgacgaa agggctgatg aggaaaatta acggatttac gattaagaaa atgatgaggg
    10681 cgagtttgta ccagtcgggg gactggccca aaaaattgcg ccatagtgcg cggccccagg
    10741 agatctccat gatggtttcc cttaccttag aaataatcaa tgatgttttt atgtttaaac
    10801 gcaaagctta acggtcagac aggagtgagg caagtcttga tagtcaaggg gaaagagatg
    10861 cagaaaatga agccttgatc ccttttttct tctttttgtc ttctatcagc gtagttagcc
    10921 ctctggtatg atgagtccaa ctttgttttg ctgtgttatg gaaatctcac tatggtcatt
    10981 aaggcgcaaa gcccggcggg tttcgcggaa gagtacatta ttgaaagtat ctggaataac
    11041 cgcttccctc ccgggactat tttgcccgca gaacgtgaac tttcagaatt aattggcgta
    11101 acgcgtacta cgttacgtga agtgttacaa cgtctggcac gagatggctg gttgaccatt
    11161 caacacggca agccgacgaa ggtgaataat ttctgggaaa cctctggttt aaatatcctt
    11221 gaaacactgg cgcgactgga ccacgaaagt gtgccgcagc ttattgataa tttgctgtcg
    11281 gtgcgtacca atatttcaac tatttttatt cgcaccgcgt ttcgtcaaca tcccgataaa
    11341 gcgcaggaag tgctggcaac cgctaatgaa gtggccgatc acgccgacgc ctttgccgag
    11401 ctggattaca acattttccg cggcctggcg tttgcttccg gcaacccgat ttacggtctg
    11461 atccttaacg ggatgaaagg gctgtatacg cgcattggtc gtcactattt cgccaatccg
    11521 gaagcgcgca gtctggcgct gggcttctat cacaaactgt cggcgttgtg cagtgaaggc
    11581 gcacacgatc aggtatatga aacagtgcgt cgctatgggc atgagagtgg cgagatttgg
    11641 caccggatgc agaaaaatct gccgggtgat ttagccattc agggacgata atcccttccg
    11701 tttaaaaagc aaacccctca aacgaggggt ttttcgttgt ttttacagat ttcccattct
    11761 cggcgggcaa cgttccagca actcgacgct gccgtcttcg ttttgctgtt ccagcatcac
    11821 atcaaatccc cataggcgat gcacatgctt cagcacttct ttgcgccccc gatccagcgg
    11881 tgcgcgatta tgtggaatgt aacgcagcgt cagcgaacgg tcgccgcgca aatccacgtt
    11941 ccagatctga atattcggct ccagattact taaattatat tgagacgata accggttacg
    12001 gatctcccga taaccttctt cattatgaat agcggaaatc tccagataat tatgccgatc
    12061 gtcatccagc acggtgaaga agcggaaatc acgcatcact ttcggtgaca ggaactggct
    12121 gataaaactc tcatctttga aatcacgcat cgcgaaatgc agcgtttcca gccagtcgga
    12181 accggcgata tccgggaacc agtatttgtc ttcttccgtt ggcgactgac aaatccgttt
    12241 aatatcctgg aacatggcga acccgagggc atacgggttg atgccgctgt accacgggct
    12301 gttatagggc ggctggaaga ccacattggt gtggctgtgc aaaaactcca gcataaaacg
    12361 ttccgttact ttcccttcat catacagatg gttaaggatg gtgtagtgcc agaaggtcgc
    12421 ccagccttcg ttcatcacct gagtctgttt ttgcggataa aaatactggc tcaccttacg
    12481 cacaatacgc agaatttcac gctgccatga ttccagcagt ggcgcatttt tctccataaa
    12541 atagagcagg ttttcttgtg gctcggacgg atagcggcgc gcttcagcaa ccgttttctc
    12601 ttcctcgcgc ttcggcaggg tacgccagag catattgacc tgactttgca gatactcttc
    12661 gcgacttttc tgccgggctt tctcttcttg cagcgagatt ttttgcgggc gtttgtaccg
    12721 gtccacgccg tagttcatca gcgcgtggca cgagtccaga agccgttcta cttcatcaac
    12781 gccataacgt tcttcgcact cggtaatata tttacgggca aaaatcagat aatcgacaat
    12841 cgaactggcg tcggtccagc tacggaataa gtaattgttt ttgaagaaag agttatgccc
    12901 atagcaggcg tgtgccatca ccagcgcttg catggtaatg gtgttttctt ccatcaggta
    12961 agcgatacac gggttagagt taatgacgat ttcataggcc agtccttgct gaccgtgctt
    13021 atacagccgt tcagtctcga taaacttttt accgaatgac cagtgcggat agttaattgg
    13081 catgccgacg ctggagtagg catccatcat ctgttctgag gttatcactt caatctggtg
    13141 cgggtaggta tccagccggt agagtttcgc cacccggtct atctctgcca gataaacatc
    13201 cagcaggtcg aacgtccagt cgggtccatc gctcaaacgt gtggtgtcct tattcataga
    13261 atcgatcgtc gccatacgcg cacctcattg ttgtcggcgc tctctgtgtg gagcacctca
    13321 tttcaagcat agaacacctg ttaaaaaccg cgtcgccgga gaattttttt ctttgcgatt
    13381 tcttattatc agagtgccac taatctgctt ctgaacggaa ttttatgctg gataaaaagg
    13441 gcgttcagca ggagatacta aagacgccat attgccgcag agtcagggag atgtgagcca
    13501 gctcaccata aaaaagccgt atgttgaata atattttcaa ctgagttatc aagatgtaat
    13561 tggattatta ttcttttact gtatctaccg ttatcggagt ggctatgcga gttgtcatac
    13621 tgggaagtgg tgtggtaggc gttgccagcg cctggtactt aaatcaggca ggacatgagg
    13681 tcaccgtcat tgatcgggag ccgggggccg ccctggaaac cagtgccgcg aatgccgggc
    13741 aaatctcccc cggatatgct gcaccgtggg cggcaccagg tgtgccttta aaagcgatta
    13801 aatggatgtt ccagcgccat gcgccgctgg cggttcgtct cgacggtacg cagttccagt
    13861 tgaaatggat gtggcaaatg ttacgtaact gcgacaccag ccactacatg gaaaacaaag
    13921 gacggatggt gcgtctggcg gaatacagcc gtgattgcct gaaagcattg cgcgccgaaa
    13981 ccaatattca gtatgaaggg cgtcagggtg ggacgctgca actgttccgt accgaacaac
    14041 agtatgaaaa tgcgacccgc gatatcgccg tgctggaaga tgctggcgta ccgtacctgc
    14101 tgctggaatc cagccgcctg gcggaagtgg agcccgcgct ggcagaagtg gcgcacaaac
    14161 tgacgggtgg tctgcaactg cccaatgacg aaaccggaga ctgccagctg tttacccaga
    14221 atctggcgcg gatggcggag caggcggggg ttaaattccg ctttaatacg cccgttgatc
    14281 aactgctttg cgacggcgag caaatttacg gcgtgaagtg tggcgatgaa gtgattaagg
    14341 ccgatgcata tgtgatggca tttggttctt actcgacggc gatgctcaaa ggcattgttg
    14401 atattccggt gtatcctttg aaaggctact cgctgaccat tcccattgcg caggaagatg
    14461 gtgcgccggt atccaccatt cttgatgaaa cctacaaaat cgccattacc cgtttcgata
    14521 accgcattcg tgttggcgga atggcggaaa ttgttggttt taacaccgag ctgttgcaac
    14581 cgcgtcgtga aacgctggaa atggtggttc gcgatctcta tccacgcggc ggtcatgtcg
    14641 agcaggcgac tttctggacg ggtctgcgcc cgatgacgcc agacggcacg ccgtttgtcg
    14701 ggcgtacagg ctttaaaaat ctgtggctga ataccggtca cggcacgctc ggctggacga
    14761 tggcttgcgg ttccggtcag ttgttaagcg atctgctctc tggtcgcacg ccagcgatcc
    14821 catatgagga tctaagcgta gcgcgctaca gccgtggatt tacgccatca cgcccgggcc
    14881 atttacatgg cgcacacagc taaggaaacg agatgacccg tccgatacag gccagcctcg
    14941 atctgcaggc attaaaacag aatctgtcca ttgtccgcca ggccgcgccg cacgcgcgcg
    15001 tctggtcggt ggtaaaagca aacgcttacg ggcacggtat tgagcgtatc tggagcgcgc
    15061 tcggggccac cgatggcttt gcattgctta atctggaaga ggcaataacg ttacgtgagc
    15121 gcggctggaa aggaccgatc ctgatgctgg aaggattttt ccatgctcag gatctggaga
    15181 tttatgacca gcaccgcctg accacctgcg tacacagcaa ctggcagctc aaagcactgc
    15241 aaaatgcgcg gctaaaagca ccgttggata tttatcttaa agtgaacagt gggatgaatc
    15301 ggttgggctt ccagcccgat cgcgtgctta ccgtctggca gcagttgcgg gcgatggcga
    15361 atgttggcga aatgaccctg atgtcgcatt ttgccgaggc ggaacatcct gatggaattt
    15421 ccagcgcgat ggcgcgtatt gagcaggcgg cggaagggct ggagtgtcgg cgttcgttgt
    15481 ccaattcggc ggcgactctg tggcacccgg aagcgcattt tgactgggtt cggcctggca
    15541 ttattttgta tggcgcttcg ccgtccggtc agtggcgtga tatcgccaat accggattac
    15601 gtccggtgat gacgctaagc agtgagatta ttggtgtcca gacgctaaaa gcgggcgagc
    15661 gtgtgggcta cggcggtcgc tatactgcgc gcgatgaaca gcgaatcggc attgtcgccg
    15721 caggatacgc cgacggttat ccgcgccatg cgcctaccgg tactcctgtt ttagtggacg
    15781 gcgtgcgcac catgacggtg gggaccgtct cgatggatat gctggcggtt gatttaacgc
    15841 cttgcccgca ggcggggatt ggtacgccgg ttgagctgtg gggcaaggag atcaaaattg
    15901 atgatgtcgc cgccgctgcc ggaacggtgg gctatgagtt gatgtgcgcg ctggcgttac
    15961 gtgtcccggt tgtgacagtg taacttgtta tcgctggatg cgaccagcgt cgtatccagc
    16021 gttacaaaac acattaaact taagattcgg cttcttcctc cgctacccga acaccaatct
    16081 tcaacacttc attatcctct ttctcggcca ccgtccagat cattccggca aattctacct
    16141 gatcaccgac aaccggtgcc gcgcctaaca actgctggac aatttcaccc agcgtttgct
    16201 gcttatcacg atattctcgc ccgtcttcca ggccatatat cagcgccaca tcagcatatt
    16261 tggcactggc ttcgagaatg aagtcaccaa agaagcgttg atctagcgcg accggtggcg
    16321 actggctgaa cagtttaccg agcgccggga gatcgcgttc ccgaccaatt acacacaaca
    16381 catcgccttc gcgcagtcgg gtgctgccgg taggatgaag caactggtta tcacgaaaca
    16441 gtgccgcaat acgcgtctct tttggcatat gcagatcgcg cagtgccgcg cccacgcacc
    16501 atttatcggc actcaactga taaacaaact gctcccacgg attttccgga tgaatatcca
    16561 gaccaacgcg tgacaccgga cgtcccactg gcggaacgac cactttggct tttttcgccg
    16621 cccacgagag tgatgtcccc tgcaagagta gtgaaaccag aaccacaaag aaggcgacat
    16681 taaagaacag tcgcgcattc tccagccccg ccatcatcgg gaacacggcc aggatgattg
    16741 gcaccgcgcc gcgtaatccc acccagctga taaacacgcg ctcacgcaga ttaaaaccac
    16801 ggaacggtag caatccggca aataccgaaa gaggacgagc gaagaatatc atccatgcgg
    16861 acaaaatgag cgccggaatg gcaataggca gcagatcgct tgggttaacc aacagcccca
    16921 gcaccaggaa catggcgatt tgcgccagcc aggcgaggcc gtcgaaattt tgcaggatgc
    16981 catagcggtt gcgaatcggg cgattaccca gcagaaaacc gcacagataa acagccagaa
    17041 taccgctacc ttccagcgcc gtggttaaag caaaaattaa aataccgccg cttaatgcca
    17101 gcaatggata taatccggcg ggcagggcga tgcgattaat catttgcagc agtaaataac
    17161 caccgccaag accgatgaca atcccgaggc caaattgctg cagaatatca accacgaaca
    17221 tccagctgac actgctttca tgctgctgga tcatcgcaat cagggtaatc gtcagaaaga
    17281 ccgccattgg atcattactg ccggattcaa tttccagcgt cgagccaaca cgttcgttaa
    17341 gccccttacc acccagcaga gaaaagaccg ctgcagcatc ggtggagccg acgatagcgc
    17401 cgattaataa gccttcaatc aaatcaagat taaacagcca cgccgccatc atgccggtta
    17461 aaccagaggt gataagtacg cccagcgtcg ccagcgacag cgccggtcct aacgccacac
    17521 gaaaggaact ggcctgagtg cgcatcccgc cgtcgagcaa aataatcgcc agcgccaggt
    17581 tacttaccat gtaggcgaag gggtaattat caaacgggat accgccgacg ccatcaaccc
    17641 ccgccagcat gccgatcgcc agaaaaataa ccagaatagg aatgccaaga cgggaagaaa
    17701 atgaactaag taatatactg ctggtgacaa ggatggatcc taagatgaaa aggctaatta
    17761 ttgttgtggc atccaacggt cggttactcc tgattacgct gtctcttata taaaccctac
    17821 catattagcg gcagagacag cgttttactt agccctgaag cgtgattttt ttacatttta
    17881 agaacaggat gaccgctaat cgtcaactga gtgccttcct gggtgttatt aagcatggct
    17941 tgtgcgccca acggcagcgt aaccgtacgt tgttcatgac caaaatcgag gccggtaatg
    18001 agtggaatcg acaggcggga acgcaaaaac gcgtacactg actccaggtt gtaacctgcg
    18061 tcataatcat tgggcgtgct gccgctaaag ctaccgagaa taatcgcctt ctgacgtggc
    18121 aaaattccag catgatagag ctgcaacagc atacgttcga cgcggaaagg atgctcgtta
    18181 atatcttcca gcaccagaat accgttttcg atttgcggca tccacggtgt accaatcagt
    18241 gaaatcagca tcgcgagatt gcctccccat aacgtgcctt cggtctgaca tgtcgggcct
    18301 tccccttgcc attcaatggt gaaggtttca ttgcgtaacg ccagccagaa atggtgctcg
    18361 gtaaaggcgt tcagttcatc cgcgccaaag tttgctacca gcatggggcc gctaaaggtg
    18421 atgacattgc catgcgccag aagaccgcac tgaatggcgg taaaatcgct atgtccgcaa
    18481 atgagcaacg ggtcatgttg ttggcgagcc accagtgcct gccagtcaat atctgccagt
    18541 aaacgactgg caccgtaacc gccgcgtaca gccagcacga tggtgttggg ggtagtcagt
    18601 ctggcaagag aattgagatc ttccagacgc tccgtttccg tacccgcaaa acgctcacaa
    18661 cgacgggcaa taacctcgac gttattgacc tgatgccccg cgtcggtaag gcgctggata
    18721 ccacgcagcg cggcgtgctg tttaatgcag taacccgatg gggcaattaa gtgaaacaga
    18781 gacatggtaa ttccttgctg acaacagaaa cgaaatgtat atcatgccgc ttaggtgtgc
    18841 cgttgtcacc tcaacggcga ttccaggcta taaggataga agaagtgaaa ttgagatggt
    18901 ttgccttttt gattgtgtta ttagcgggtt gttcatcgaa gcatgactat acgaacccgc
    18961 cgtggaacgc gaaagttccg gtgcaacgtg cgatgcagtg gatgccaata agccagaaag
    19021 ccggtgcagc ctggggcgtc gatccacaat tgatcacggc gattatcgct atcgaatcgg
    19081 gtggtaatcc gaacgcggtg agtaaatcga atgccattgg tttgatgcag ttaaaagctt
    19141 caacctccgg acgtgatgtt tatcgccgta tgggctggag tggtgagccg acgaccagcg
    19201 agctgaaaaa tccggagcgt aatatttcaa tgggggcggc ttacctgaat attctggaaa
    19261 ccggcccgct ggcaggcatt gaagatccga aggtactgca atatgcgctg gtggtgtcat
    19321 atgctaacgg ggcaggtgcg ctgctacgga ctttctcgtc agatcggaaa aaggcgatca
    19381 gcaaaatcaa cgatctggat gctgacgagt tcctcgatca cgtagcgcga aatcatcctg
    19441 cgccgcaggc tccgcgctat atctacaaac ttgagcaggc actggacgca atgtaaatca
    19501 gtcgcgcact ttgtccgctt tctcccgggc ttcccgctcg agagagaaaa taatccgctg
    19561 taattgccgc tccaccgtcg ggctgacgtt aagaaaacgg aagctcagac ggggagtggt
    19621 gatggtttca ttcttcccat caatcacttt gcgctcgctg atggagatta actgggcgtc
    19681 aaagtgaaaa acaccccatt gccccatgtt gacttcaatc tgagcgaagc gcatgccttc
    19741 gtgcaatccg gtaggctttg ctgtttccag taatgcgccc atgccgccta acgacaaatc
    19801 atacaggcgg aaacgtaacg tactgttatc cgccagtttg gtctggcaaa aataaggcgg
    19861 atggagtggg gcggtgatgc ggaaatatcg gcgtcgttgt acaaaccata aggtgggagg
    19921 cggtacggta ataaatgccg gaagctgcaa gtattcactc tgctgtagtt gttcaacagt
    19981 aaactcgact ttcgcaccct gagtttcggc ggtaatgata atgtgctgcg cctttagcac
    20041 ggcgttgtta tcttcggcct ggctgccgaa atccagcacc agtttatccg gagttattgc
    20101 cagtattttg ctgatcagct gcccgccatt ccagctgata cgcaaaggaa ttgcggcttt
    20161 gtgcaaatcg cgtaacacgc ccaggacggc taacggattt tgtttcagga actgctcatg
    20221 gtaatgactc acgcggaaaa actcctgact gacaaactgt ctttggttta tcggtcacag
    20281 ttaacaaaac ttaatacaaa tgcgtgaata ttttttacat gttgttctta aatcagccga
    20341 catacgccca gcatcgattc cgcccttgcc tatacttaga gcgttgacgt aagcatttct
    20401 tgcgtccgat tcatcgaaac gagggcctga acatgggaat tattgcctgg attatttttg
    20461 gcctgatagc cggcattatc gccaagctaa tcatgccggg gcgtgatggt ggtggatttt
    20521 tcctgacctg tattctcggg atagtcggtg cggtggtcgg cggctggctg gcgaccatgt
    20581 ttggcattgg cggctccatt agtggtttta atctgcacag cttcctggtg gcggtggtgg
    20641 gcgctatcct ggtgctgggc gtattccgcc tcctgcgaag agaataagat tttcataagg
    20701 cggatagcga tacagatgcc gctatccgct ttcacatcag aacgtatact cgatacctgc
    20761 ccagaaattg cgaccttctt caataaaccc ttcactgtag gcatatttgg tatcaaacag
    20821 gttattgacc gacgcattga cgctgaagcc atgacctaag gtgtaatcgg ctcgaatgtg
    20881 ggtcaccgca aaaccggcgg ctttttgtga accgtcactg ttgctgtagc tggaggaacg
    20941 cgcctcttcc gacagcgtta cgcttaacgg ctcccacggt ttgagagtca tccatgcggt
    21001 cattgtctgc gttggcagat cggttatctt gccgatgtct ttgcgtttgg catcagcgtg
    21061 gatcagggcg tagctcaatc ctacatccag tatattgctg attttcccct taataccggc
    21121 atccagaccg ctgtaatcca ccgtgccgct gttctgattt tgaatggtat cggcatcgat
    21181 attgtgcgag aggatggcat cactcactcg gttatagtaa acgctgacct caaaccccca
    21241 gtcgtgcgtg aaggcaccat tccaggttaa atccaccccg cgcgcgcgtt ccggtttgag
    21301 ctgcgggtta acaatcgcta tctggttata cgcaggtttg gacgtggtat agcgttcttt
    21361 cagcgtcgga aagcgtgtgc ggtcatagta cgaaagcgcc agcgtgtctt cattggcaaa
    21421 gtggtatttc cccatcacct gccagttaaa agctgactga ttgttgtcgt cataatgggt
    21481 gatgcttcca tctttctcat gttttttcgc ttctacgcta tcgcgccagt catagctgat
    21541 tccagccacg acatcgacat tatcggcagc agcccattga tattcactgg cgagcgacca
    21601 ggtacgatct tcatagcgat cgtaagcggc gtgcggcgca cctttttccc ggtgtacgtc
    21661 atctttccag ttaacggcaa acgacagcag atcgttttca cgcacatcgg ctgccagttg
    21721 taacccggca ccgtcgctgt aatcggaata atggctgtag ctgccttttt tatttttcaa
    21781 atcagccagc gagttgtaca tcatcagcgt attttcaaag gtgtcgcgat atagccgact
    21841 tttcagggta aaacgatcgt ttagttgggt cgttccctga taataaaaac tttctttgtc
    21901 atactctggc cactgccagt agcgtgattt ttgaccacta tttccactgt atggcgggtt
    21961 atctttttca ccatcctgct taatgtaagt caatgtgtat tcatcgtttt cgcgtggtgt
    22021 aaaaccgagt ttcacaatgc cgcgtttatc atcagccgat gaattaatca tcttgccgtg
    22081 tttgcctgca atatcattat ttacaccatg cggcaggccg agaaaatcct gctttagctg
    22141 gctaccgctg acttgcaaat accccagatc gctgctggcg gcaaatgaag catgcatatc
    22201 gtaggcattg tcctggctac ggctccatcc ctggcgatat cccagatttg cttccagagg
    22261 ttttgttggc ttctgggtgg tgatattaat ggctccgccc atctgattag gcccctgaag
    22321 cagcgacgaa taccctttgg aaacttcaac tgcccccaaa ttgttggtca gaatccgcgc
    22381 cagatcgagg ttgccgtcat agggaacata aatgggcaca ccgtcgaaat agaccggcac
    22441 ctgacgacta tcaaagccac gaactttgac ctgttcttcg ttgcggctac ctgacttttg
    22501 cagcaccacg ccggggacga cacttaatgc ctgagcgaca ttctgtttat ccagggcctt
    22561 catggtgggt tgatcgagaa cggtggtcgt cgtcgatgat accggactgg accatacggt
    22621 cagcgtgtca ctctcctgag ccgctacggc ctgctgggta aaggcaagtg ataatgctgt
    22681 gcagagataa cgttttttga gtctcataga ataaattccg tttaacaaag aattaaatag
    22741 aagctgcggg ttgcagactg actttaatgt cagctggtgt cgcgtaataa ggcgcggagg
    22801 taataaaaag gcgaatgccg cagtcgaggt aatttttgag tgttgtcaga ttaattccgc
    22861 cggtcagagc cagcgtgcag tggggagcca gcgacggtgc tatttgcgca atttcagttg
    22921 cctgttgcgg actaaatttg tcgagctgaa gtacgtctgg ttgcgcgcgt aacgcggcga
    22981 ttgcctcttt cggcgtgtcg gcttcaacaa ctattttctt ctcgggtgcg tggcgacgta
    23041 actgattgat tgcgcctgac cagtcctgat tgtcatgaag aaaatggcgg tggttggcaa
    23101 acaataatat ggtttccgca catccggcac gatgaatcag tcctccggca gccagaattg
    23161 cctgcgaggc cagtaagcgg gtgcccggaa ttgcttttcg ggtgcaggcg atattgccat
    23221 cagggtaacg ttcacgaagt aacgccagca tttgagcgag ataatcagaa acaccgcaac
    23281 tccactccag cacattctgg actgccttcc atccctgatg aagtgctgcg gcattacctt
    23341 gcgcacggat tagccgctga cccgcgttcg cttgagaacc gtcgctgacc gcgtcatcaa
    23401 tagttaatcc cagcgtagtt aacatcttac acgcgacaga aattccgctg acgcagccgc
    23461 cctgacggag aaaaaactct atatagccat gctggtgtcc aatatttaat gctcgcgtgg
    23521 tcaggtcacc gccctggata tcttccagca gtaacgcatc gatttgtgcc tgagaaagga
    23581 aaatcatcgc agtgtctcct gcggtacgct atcccaccag atgagcgccc agtcgcggct
    23641 ggcaggggcg agggcacgag catcttgttg ttgataccag cgatataacc gctcacgctc
    23701 atcgtcgtta agcgcgccca gactccagct gacgttctgc tcaaaacgtt cccaggtgct
    23761 gttatcctgt tggcaatttt gtccgcggat aaaatcaacg tgagcataaa ttcccatctg
    23821 atacaggacg ttgagcgcaa agatatagtt gggtaattcg accacttccc gaccagccgc
    23881 ccgctgaatg gctggagaga cgaacgaggt gctgaccaga tgagtcgtat agacgcgaag
    23941 ccgtgcctga ttattcagtt tgctcattgc ctggcgcata tcagccacca gagtggagcg
    24001 ggaagccacg gcaatatcgc agcgtggcag gtcactccag tcctcttccc aggcgcgctg
    24061 gatccagtgg acattgtctg cctttagcgc ggcggcgcga cgggcggcaa cgttcagcat
    24121 cccctgactg taatcaacgc cataaatcgt cgtgagtttg tccgccagcg ccagcgatac
    24181 ggtacccggt ccgcagccca tatcgaataa ggtttgcgcg ccttgcaggt caatttttga
    24241 ggtcagttgt tgcaggtagc tgtccgtcgg cgaggcgcaa ttttccgcca ttttttccgc
    24301 ccgttgatcc cagtgatccg gcgttttttc tgtgcgatgg gccagcctga gttgctgaag
    24361 gtaaaggtct gcaaaatcaa tatcgtcaat gagcatgatt tttccttagt gcttaattgc
    24421 agagaggtga tggtgaatct ggtcggcaga aacccggtac agggcagcca gttttttcgt
    24481 tgttaactgt tcggtcggta agccttgtgt tactcgtcca tctggctcta cctggataat
    24541 gctgtcggca atggcgttgg catggagcgg atgatgagtc gacattaaca ttgtcatgcc
    24601 gttatttttg agctgcgcca gcgtgtccag aagttggatc tgatgaccga aatcgaggct
    24661 ggaggcaggc tcatccagta ataacaagcg cggttgctgg acgagtgcac gggcgattaa
    24721 agccagctga cgttcaccgc cgctaagcgt attccagcgt cgtgccgcca gatgcaaaat
    24781 gcccagcttt tccagttgct ccgttgcttt caggcgttct tgcttaccgg gaacggaaaa
    24841 agcgcctatg gtgggggcca ggcccatcaa caccatatcc agcacactaa aagcaaaaat
    24901 gccgtcgtgc gcctgcggca cccaggcaat agcctgcgcg cgctggcgat ggctgagtgt
    24961 ctgcacagag aggctatcca gtaagatctc gccttttaat aacggtataa cgccaagaat
    25021 ggagcgcatg agtgtggttt tgccgcaacc attggctcct aacagacaac aaattgttcc
    25081 ttgcgacagg gtgaaggaca cgtcgcgtag caccggatgg cgggagtaac ccagagacgc
    25141 atgacggacg gcaagcaaag tcactggcga cctccgcgta acaacaaaaa caggaaaaac
    25201 ggtgcaccaa caaaagcggt cagaatgcca agtgggattt ctgttgttcc aatgctgcgt
    25261 gccagcgtat cggtaagcag gagcagaatc gcgccagtac acattgccat gggcaacagc
    25321 tgctgatggt tatgaccggt caacaagcgg gcaatgtgcg gaacgaccag tccgacccaa
    25381 ccaattatgc ccgctattgc taccgtgctg gcggtgatga gtgtggcgca gacaatcaat
    25441 ccaaagcgca gacgtgtcac gttcagtccc agcgaacggg cttcgtcgtc ggaaagcgtg
    25501 agtaaattca tgcgccagcg gagaaaaaac aggggcaagg agcctatgag aatgatcggt
    25561 gcagcatagc agagatcgcg taaggtcacg gtggacagcc cccccagcaa ccagaaggta
    25621 attgacggta actgggtgta aggatcggcc agcgttttta ttagcgatat ccccgctccg
    25681 cacaacgtgc ctaacgcaat acctaccagc accagggtta agatcggatc gtggcgggtc
    25741 acccgacgcg taatcaacca gacgcccgcg accaccatca gcccgccgca aaatgcgtac
    25801 agctggatgt aaacaatgga taatccccac aggattgccg cgcaggcacc aagtccggca
    25861 ccggcggaga cgcctaaaat atccggtgac actaagggat tacgcaacat tccctgatag
    25921 gtggttccgg cccctgccag cgccgcgccc agaaacagcg cagcaagaat acgcggcacg
    25981 cggatttgcc agaagacaat ttgttcctgc acaggaacat tttcttgacg aagaatgagc
    26041 gccagcagtt gctgcatatc gagatgataa gccccactta cggcggcaaa agtgatactg
    26101 atagctgcca gcagcaaaat acatccgttt acggttctca ttagctcgcc accaattttt
    26161 gatagtcggc gtcactgagt gaacaatgcc agaacagttg ggcgtaatgc tgtatgtcac
    26221 ttttaaactg gcgattgatg gcgggatcca gccaggcgtg aagtctgcgt aatcccagaa
    26281 gacggttgat tcccggcggg gcatccagcc agccaaaggg caggccactt aaaaataaga
    26341 tgcgttgttc cgcaacggct ttcacgccct gccagagagg atcacgacga ataaaatctg
    26401 cagtaacggc ctcctgaacc agaataatat ccggctgcca gcgcaggaga ttttccatgg
    26461 aaacctgtgt cagaccgtgg cgatcggcta tttgcgcgac gttgtgcaaa cctaataatt
    26521 ccgccgcctc ggtatgcaac gaaccctgta agcccgtttc cagccctcga ggaccgcgcg
    26581 cagcataaaa gctgagatta gcggcgggtg aggtggcgaa tgcctgagct tcaccaacga
    26641 agcggctggc gagattggct tgttctgcgg cgcggtgctc ttcgcctaac gttttgccaa
    26701 gcgttgttaa ctgttctgct gattgttcca gtttcccgtt aagcaataac cagggtattt
    26761 gtgtctgttc gctaacctgc cgtgcctggg agatccaggt ttcatccgta ttgccgcaat
    26821 caacgaccaa atcgggatgt aacgccataa gcccttccag cgagagtgtg ctggcgcgcc
    26881 cggtgagtcg tcccagcctg gggaactggc gaatgtgctc tggcaatgga attaatgcct
    26941 gacgggcaaa atcaaaagag gaaaaaccga ccattttttc tggcgcgaca gccagcaata
    27001 gtaaatcggc gggcgcgcct gcgctgacga tgcgtgtaat gttttgggct tcgggaagcg
    27061 tccgtgaggc ccagaatttg actgattgtg caaaggacgg cagatgatgc aggaacaatg
    27121 tcgacgcaag aatctgtgta aacctccgtc tggttagtga cataagtaat atcctgataa
    27181 ttaaaaccgc tatatactta cacatatagc gaaaaggatc atgtttgatg tcaaaatttg
    27241 tctaaagaga cagaatgatt tgcttgtttc ttaaataaca ctttgttcca caagaaaatt
    27301 gtcagggaga atggggagtg ggggaaa
//
LOCUS       DAPHCV010000070        26858 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.070, whole genome shotgun sequence.
ACCESSION   DAPHCV010000070 DAPHCV010000000
VERSION     DAPHCV010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.070"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(169..2436)
                     /gene="adiA"
                     /locus_tag="RH033_002881"
     CDS             complement(169..2436)
                     /gene="adiA"
                     /locus_tag="RH033_002881"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HDV2992036.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESERYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEVQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(2635..3543)
                     /gene="melR"
                     /locus_tag="RH033_002882"
     CDS             complement(2635..3543)
                     /gene="melR"
                     /locus_tag="RH033_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HDV2992037.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3826..5181
                     /gene="melA"
                     /locus_tag="RH033_002883"
     CDS             3826..5181
                     /gene="melA"
                     /locus_tag="RH033_002883"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDV2992038.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAVYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            5284..6705
                     /gene="melB"
                     /locus_tag="RH033_002884"
     CDS             5284..6705
                     /gene="melB"
                     /locus_tag="RH033_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HDV2992039.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYIGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPALFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(6844..7473)
                     /locus_tag="RH033_002885"
     CDS             complement(6844..7473)
                     /locus_tag="RH033_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HDV2992040.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISANDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGHKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARLHCHQLRRYGLITR"
     gene            complement(7596..9242)
                     /gene="fumB"
                     /locus_tag="RH033_002886"
     CDS             complement(7596..9242)
                     /gene="fumB"
                     /locus_tag="RH033_002886"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDV2992041.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(9320..10660)
                     /gene="dcuB"
                     /locus_tag="RH033_002887"
     CDS             complement(9320..10660)
                     /gene="dcuB"
                     /locus_tag="RH033_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HDV2992042.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISIPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(11231..11950)
                     /gene="dcuR"
                     /locus_tag="RH033_002888"
     CDS             complement(11231..11950)
                     /gene="dcuR"
                     /locus_tag="RH033_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HDV2992043.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(11947..13578)
                     /gene="dcuS"
                     /locus_tag="RH033_002889"
     CDS             complement(11947..13578)
                     /gene="dcuS"
                     /locus_tag="RH033_002889"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HDV2992044.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            13758..13988
                     /locus_tag="RH033_002890"
     CDS             13758..13988
                     /locus_tag="RH033_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HDV2992045.1"
                     /translation="MDQALLDRGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            14000..14272
                     /locus_tag="RH033_002891"
     CDS             14000..14272
                     /locus_tag="RH033_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV2992046.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            14499..14795
                     /gene="ghoS"
                     /locus_tag="RH033_002892"
     CDS             14499..14795
                     /gene="ghoS"
                     /locus_tag="RH033_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HDV2992047.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMSFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            14823..14996
                     /gene="ghoT"
                     /locus_tag="RH033_002893"
     CDS             14823..14996
                     /gene="ghoT"
                     /locus_tag="RH033_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HDV2992048.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(15115..16632)
                     /gene="lysS"
                     /locus_tag="RH033_002894"
     CDS             complement(15115..16632)
                     /gene="lysS"
                     /locus_tag="RH033_002894"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDV2992049.1"
                     /translation="MSERETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPLK"
     gene            complement(16869..18326)
                     /gene="dtpC"
                     /locus_tag="RH033_002895"
     CDS             complement(16869..18326)
                     /gene="dtpC"
                     /locus_tag="RH033_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HDV2992050.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSALRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(18385..20532)
                     /gene="cadA"
                     /locus_tag="RH033_002896"
     CDS             complement(18385..20532)
                     /gene="cadA"
                     /locus_tag="RH033_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HDV2992051.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAADDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(20612..21946)
                     /gene="cadB"
                     /locus_tag="RH033_002897"
     CDS             complement(20612..21946)
                     /gene="cadB"
                     /locus_tag="RH033_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HDV2992052.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(22312..23850)
                     /gene="cadC"
                     /locus_tag="RH033_002898"
     CDS             complement(22312..23850)
                     /gene="cadC"
                     /locus_tag="RH033_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HDV2992053.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTTTPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLETRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSV
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLQSLSKALNQPWPQRMQEMLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLDRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(24599..25441)
                     /locus_tag="RH033_002899"
     CDS             complement(24599..25441)
                     /locus_tag="RH033_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDV2992054.1"
                     /translation="MSDITISRPEVVTGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKAAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEHDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQTDRLVDLERMLHLFSGKPIPDNR
                     ENITIRLDGHIQSVQGKERYEDEMFIIKYFKKGSAHITFKRLELIDRINDIIARYFPS
                     VLSA"
     gene            complement(25526..25723)
                     /locus_tag="RH033_002900"
     CDS             complement(25526..25723)
                     /locus_tag="RH033_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992055.1"
                     /translation="MMPLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSVALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(25751..25948)
                     /locus_tag="RH033_002901"
                     /pseudo
     CDS             complement(25751..25948)
                     /locus_tag="RH033_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(25945..26322)
                     /gene="cbtA"
                     /locus_tag="RH033_002902"
     CDS             complement(25945..26322)
                     /gene="cbtA"
                     /locus_tag="RH033_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HDV2992056.1"
                     /translation="MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPGAKQ"
     gene            complement(26412..26780)
                     /locus_tag="RH033_002903"
     CDS             complement(26412..26780)
                     /locus_tag="RH033_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HDV2992057.1"
                     /translation="MSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYMAVYPTPETKK"
BASE COUNT         6850 a         6363 c         6630 g         7015 t
ORIGIN      
        1 ctaaaaaaag gataaataac aggcaaaaaa aagcgcgggg tgggaccgcg caaaaagcat
       61 gacatttttt gtttgcagca atttttatga aaacgttgcc atacttaaaa ggcctcaagc
      121 caggcggcgt aatgttattt aacaattacg ccttcagcgg aatagtggtt acgctttcac
      181 acacataacg tggtaaatac cgtcaataat ttctgtccct tctgtttcgt gttcaaatcc
      241 agggaaatga tggtcccagg attgcagcga gcgtaaataa cttacttgcg gactgttttt
      301 atcgccgaag ttttcaccag acagcagcat cgggattcct ggcggatacg ggataactga
      361 gtttgccgcg atacgtcctg gcagattttc aatggatacc agttcgacat tgttgtcgac
      421 aatcgcgttg tacgcttcac gcggggtgat ttccgccacc ggtaggccgg aataggcttc
      481 attcaaccgt gcgccagggt tgttttcttt cagccaggca aacatggtgt cacccagatc
      541 gtgaatcccc atgttcgcgt aagtgtcagg atattgttca acaagttccg gcatcacctg
      601 cgccagcggt gtgttggcat catagtggcg tttgaaggag caaagggtgt taaccagagt
      661 cccccatttc ccacgggtca cgcccataga gaacaggaac ataatctgga agtcagtggt
      721 acgggtaggc acaatgccgt ggcgaccaag ccaggctgtg accagcgccg ccggaacgcc
      781 ggtttcttcc agttcaccat cttcacccat tcccggagca aggatgctga ctttaatcgg
      841 gtcgagcata ctccagttat ccggaatatc tttgaagccg tgccagcttt cgcccggatg
      901 catgacccag cagtcctgaa cggtggtcag cagtttggtt ggtgcgtcag caaagtcata
      961 ggttttgccg gtttgtgggt cggtgacgac ttctttgttc cacggtttga agaaccagct
     1021 accgtcagcg gtgaactctt tatatagccg cgccatcgcc tgacggaaat caaccgcttc
     1081 gtcgatcact tcctgggtca gtgacaggcc gctgttgccg tccatcatcg acaccgccac
     1141 gtcgttggat gcgcagatgg catacagcgg ggaggtggtg gcatgcatca tgtaggcctg
     1201 gttgaagcgg gagaagttaa tcgccccacg accttcacgt acatgaatat aagaggcctg
     1261 tgacagcgca ttcagcagtt tgtgggtgga gtgggtggcg aaaacggtag gaccgttgtg
     1321 atcgccaggt tcgccgcgca tggcatagtg atcggcatag atcgggttga aacgtgcata
     1381 gccgtaccag gcttcgtcaa agtgcagacg atcggaggtt ttttccagca gatcctgcac
     1441 ttctttagcg ttataacaca cgccgtcata ggtacagttg gtcaccacgc agtaagacgg
     1501 tttttgcccg gctttgtctt tagtcagcgg gctttcactg attttcttct gcaaggtttc
     1561 aggttgcatt tcctgcggat agattggccc gataatgccg tagcggttgc ggcttggcac
     1621 catgtagacc ggtttcgcgc ctgtcagcat caagccttgt tcgatggatt tatggcagtt
     1681 acggtcaacg accacgacat cgttatcggt catgcaagcc tgcatgatgg tgcggttaga
     1741 gccggaagta ccgacgacta ccgaccagga gcgatcggca ccaaatacgc gtgcggcata
     1801 tctttcgctt tcgccaaatg cgccagtatg gtcaagcaaa gaaccgaggg aagtgcgttc
     1861 gatgcccatg tcggtgcgga acagattttc accatagtag tcatggtaga aacgtccggc
     1921 gggtgttttg gtaaaaccaa cgccgccctg gtggcctggc gctgcccagg aatattcatg
     1981 gatgtcgcta tatttcatca gcgcgctgaa cagtggcggc aacagttgct ggcggtagcg
     2041 ggtcatcgcg gcaacggcgc gtccggcgat aaagtcggcg gtatcttcca gaatccaggc
     2101 gaattcatcg acaagctcca gcagatcccg gtccattgcg gcgagggctt tttcccgatc
     2161 gcccaacagg aagaccggca cgttttgttg gcgctcatga agcttaccga tcaattgtct
     2221 gacgttttga tgttcgtccg gatgttccat ttgatagctg aacatcaggc agtcaatggc
     2281 ttcgtttgaa gagagaatgg caaaaccatc atcaaaggag gtggatttaa tcacggtaac
     2341 attttgctgg cttaaagcat ctgccagacg ctcaacggcg ttaccgaccc aggtgtcttg
     2401 atggagaaac tcgctttcaa caattaatac tttcatcatt gcttacccgg ttatgaagga
     2461 atcttcgttg cgggcaagta tcttccggcc ttttcgggct gtaaagcgcg tgaaaaacaa
     2521 acatttttga aatatttgtt tttataaaca atatgttatg tgattatttt aaattatttt
     2581 cactgcttat tatcgacggc taaactattt ttttggctga tactgatatc gtctttagcc
     2641 gggaaacgtc tggcggcgct gttggctaag tttgcggtat tgttgcggtg acatgccgac
     2701 atatttgccg aacgtgctgt aaaaacggct actcgaacga aagcctgccg tcagggcaat
     2761 atcgagaata cttttatcgg tatcgctcag taacgcgcga acgtggttga tgcgcatcgc
     2821 ggtaatgtac tgtttcatcg tcaattgcat gacccgctgg aatatcccca ttgcatagtt
     2881 ggcgttaagt ttgacgtgct cagccacatc gttgatggtc agtgcctgat catagttttc
     2941 ggcaataaag cccagcatct ggctgacata aaattgcgca tggcgcgaga cgctgttttt
     3001 gtgtgtgcgc gaggttttat tgaccagaat cggttcccag ccagagaggc taaatcgctt
     3061 gagcatcagg ccaatttcat caatggcgag ctggcgaatt tgctcgttcg gactgttcaa
     3121 ttcctgctgc cagcggcgca cttcaaacgg gctaagttgc tgtgtcgcca gtgatttgat
     3181 caccatgccg tgagtaacgt ggttaatcag gtctttatcc agcggccagg agagaaacag
     3241 atgcatcggc agattaaaaa tcgccatgct ctgacaggtt ccggtatctg ttagttggtg
     3301 cggtgtacag gcccagaaca gcgtgatatg accctgattg atattcactt tttcattgtt
     3361 gatcaggtat tccacatcgc catcgaaagg cacattcact tcgacctgac catgccagtg
     3421 gctggtgggc atgatatgcg gtgcgcgaaa ctcaatctcc atccgctggt attccgaata
     3481 cagcgacagc gggctgcggg tctgtttttc gtcgctgctg cacataaacg tatctgtatt
     3541 catggatggc tctctttcct ggaatatcag aattatggca ggagtgaggg aggatgactg
     3601 cgagtgggag cacggttttc accctcttcc cacagcggcg aggggagcgt ccgagcatca
     3661 tgaggccgaa aactctgctt ttcaggtaac ttattcccat aaactcagat ttactgctgc
     3721 ttcacgcagg atctgagttt atgggaatgc tcaacctgga agacggaggt tttctgcaga
     3781 ttcgcctgcc atgatgaagt tattcaagca agccaggaga tctgcatgat gtctgcaccc
     3841 aaaattacat ttatcggcgc tggttcgacg attttcgtta aaaatattct tggtgatgtg
     3901 ttccatcgcg aggcgctgaa aacggcgcat attgccctga tggacattga tcccacccgc
     3961 ctggaagagt cgcacattgt ggtgcgtaag ctgatggatt cagcaggggc cagcggcaaa
     4021 atcacctgcc acacccaaca gaaagaagcc ttacaggatg ctgattttgt cgtggtggca
     4081 tttcagattg gcggttatga accttgcacg gtgactgatt tcgaggtctg taagcgacat
     4141 ggtctggaac aaaccattgc cgatacgttg gggccgggcg gtattatgcg cgcgctacgt
     4201 accattccgc atctgtggca aatttgcgag gacatgacgg aagtctgccc cgatgccacc
     4261 atgctcaact acgttaaccc aatggcgatg aatacctggg cgatgtatgc ccgctatccg
     4321 catatcaaac aggtagggct gtgccattca gtgcagggaa cggcggaaga gctggcgcgt
     4381 gacctcaata tcgacccggc tacgctgcgt taccgttgcg caggtatcaa ccatatggcg
     4441 ttttacctgg aactggagcg caaaaccgcc gacggcagtt acgtgaatct ctacccggaa
     4501 ctgctggcgg tttatgaagc agggcaggca ccgaagccga atattcatgg caatactcgc
     4561 tgccagaata ttgtgcgcta cgaaatgttc aaaaagctgg gctacttcgt cacggaatcg
     4621 tcagaacatt ttgctgagta cacgccgtgg tttattaagc caggtcgtga ggatttgatt
     4681 gagcgttata aagtaccgct ggatgagtac ccgaaacgct gcgtcgagca gctggcgaac
     4741 tggcataaag agctggagga gtataagaac gcctcccgga ttgatattaa accgtcacgg
     4801 gaatatgcca gcacaatcat gaacgctatc tggactggcg agccgagtgt gatttacggc
     4861 aacgtccgta acgatggatt gattgataac ctgccacaag gatgttgcgt ggaagtagcc
     4921 tgtctggttg atgctaatgg cattcagccg accaaagtcg gtacgctacc ttcgcatctg
     4981 gccgccttga tgcaaaccaa catcaacgta cagacgctgc tgactgaagc cattcttacg
     5041 gaaaatcgcg accgtgttta ccacgccgcg atgatggacc cgcatactgc cgccgtgctg
     5101 ggcattgatg aaatatatgc tcttgttgac gacctgattg ccgcccacgg cgactggctg
     5161 ccaggctggt tgcaccgtta aaacgcgact aaacgctact gcgccggggg atttattccg
     5221 gcgcacacct ctgacgatac caataacaga aggcgggcgt tggtaacagc gacccgatac
     5281 cctatgagca tttcaatgac tacaaaactc agttatggat ttggagcgtt cgggaaggat
     5341 tttgcgatcg gcattgtgta tatgtacctc atgtattact acaccgatgt cgtcgggctg
     5401 tctgtgggtt tggtcggtac tttgtttctg gtggcgagga tctgggatgc tattaacgat
     5461 ccgattatgg gatggattgt aaatgctacg cgatcgcgat ggggtaagtt caaaccctgg
     5521 atcctgatcg gtacgttggc aaactctgta atcttatttc tcctctttag tgcgcatctg
     5581 tttgaaggta ctactcagat tgtctttgtt tgcgtgacct acatcctctg gggcatgact
     5641 tacaccatta tggatattcc cttctggtcg ctggttccaa ccataacgct cgataaacgt
     5701 gagcgcgaac aactggttcc ttatccgcgt ttttttgcca gtctggcagg ctttgttacg
     5761 gcaggtgtga cgctaccatt tgttaattat atcggcggtg gcgatcgggg atttggcttt
     5821 cagatgttca ctctggtact gatcgccttt tttattgttt caaccatcat cactctgcgc
     5881 aatgtgcatg aagtcttttc gtcagacaat caaccgtctg ctgaaggaag ccatctgaca
     5941 cttaaagcca tcgttgcgct tatttataaa aacgatcagc tttcatgcct cttgggtatg
     6001 gctcttgctt ataatgtagc cagcaatatt attaccggct ttgctatcta ttatttctca
     6061 tatgttatcg gtgatgcgga tctgttcccc tattatctgt cgtatgcggg agctgctaac
     6121 ctggtgacgt tagtattctt cccacgctta gttaaatcat tatcccgacg cattttatgg
     6181 gccggagcat ctattcttcc ggtgttaagc tgtggtgttc tcctgttaat ggcattaatg
     6241 agctatcaca acgtcgtcct cattgtgatt gcgggtatct tgctgaatgt gggaacggcg
     6301 cttttctggg tattacaggt catcatggtg gcagataccg ttgattacgg tgaatataaa
     6361 ctgcacgtac gctgtgaaag catcgcttac tccgtgcaga ctatggtggt gaagggcggt
     6421 tcagcctttg cggctttttt cattgcggtt gtgttaggga tgattggcta tgtaccgaat
     6481 gttgaacagt ctacgcaagc cctattaggt atgcagttta ttatgattgc tctacctgct
     6541 ctgttcttca tggttacgct gattctctac ttccgtttct atcgcctcaa tggtgacacg
     6601 ctgcgcagga tccagatcca tcttctggat aaatatcgca aagtaccgcc cgagcctgtt
     6661 catgctgata ttccggtcgg tgcagtgagt gatgtgaaag cctgacgtgg acaatagcta
     6721 acgaaaatgg cctgatgcga tatgtttatc aggccacgtc ttaatgtaat gctttggatt
     6781 tgcaaatttt gtagaccgga taaggaattc acgccgcatc cggcatcaac aaagcgcacg
     6841 ttgtcatctg gttatcaagc catagcgccg taactgatga cagtgaaggc gagcgaggaa
     6901 tatcaccgtc gttaatgcgc caagcagcgc gcagaacata tcggattgcg tgtcccactg
     6961 gtcgccctgg gtgccgagaa aatcatccgc tccctgaccc attgccagcg ctgcccacca
     7021 ctcgattaat tcatacatcg cgcttatcgc cagcgccacg cagcagacca gaaacgccac
     7081 catcttatgt ccgcgaacgt acatcccgcg cacaaggatc tctcgtgcca ccagtgcagg
     7141 caccagcccc tggaaaaaat gccccagttt gtcatacgga ttacgactca accccagcca
     7201 ttcctgcacc tcaaaaccaa cggggacttt cgcgtaggtg tattgtccgc cgaccatcag
     7261 gatgatggcg tgaaagaaaa tgagcgtata gagcagcggc gttaacggat aacgtttagc
     7321 ggtggcaagc agtagcggca cgacaataat caccggtgtc acttccatca gccaggtgag
     7381 tttgtcattg gccgaaatgc cggtataaat caggattaac gttagcgcca gtgcgccggt
     7441 gttaagaatt aacggcttga gtgtgcgggt catggtgcgt tcgcaagtca gggaaaatac
     7501 tgactattca ccagcaagcg tgaaattaca atcgcaaaag aggtggccca gggggatcac
     7561 ctggcagcat gctgccaggc gctgggccga agaggttact tagtgcagtt cgcgcactgt
     7621 ttgttgacga tttgctggaa gaagtcgttg cctttgtcat cgaccaggat aaacgccggg
     7681 aaatcttcta cttcgatttt ccagatagct tccataccca gctccggata agcgacgcat
     7741 tccagatgct tgatgctctg ctgcgccagt accgccgccg gaccgccgat actaccgagg
     7801 tagaagccgc cgtgtttatg acacgcgtcg gtaacctgct gactgcggtt acctttcgcc
     7861 agcatgatca tgctgccgcc gtgggattgc agcagatcca cgtaggagtc catacgacct
     7921 gcggtggttg ggccaagtga acctgatgga taaccggcag gcgttttcgc cggacccgcg
     7981 tagtagatcg ggtgatcttt gatgtactgc ggcagttctt taccggcgtc aatcagctct
     8041 ttcagcttgg cgtgtgcaat atctcgaccc acaataatgg tgccggtgag cgacaaacgg
     8101 gtggataccg ggtattgcga aagctgggcg aggatctctt tcatcgggcg gttaaggtca
     8161 actttcaccg cttcgccttc accggcctgg cgcagttctt gtggaatgta ctggcctggg
     8221 ttgtgttcca gtttttcgat ccagatacct tcgcggttga ttttcgcttt aatgttacgg
     8281 tctgcggagc aggagacgcc catgcctacc gggcaggatg cgccgtgacg tggcagacgg
     8341 ataacgcgaa tgtcgtgcgc gaagtattta ccgccaaact gcgcgccaag accgagtttc
     8401 tgggcctctt ccagcagttc ctgttccagc tggacatcgc ggaacgcctg accatgttcg
     8461 ttcccttccg tcggcagttc atcgtaatag tgagcgcttg ctaacttgac ggttttcagg
     8521 ttggtttccg cagacgtacc gccaatcaca aacgcgatat ggtacggcgg gcaagctgcc
     8581 gtaccgaggg tacgcatttt ctcgacgagg aagtttttca gtttgccggg cgtcagcagg
     8641 gctttggttt cctggtagag atacgttttg ttggcagagc cgccgccttt agcgacacag
     8701 aggaatttgt actcatcgcc atctaccgcg tacaggtcga tttgcgcagg caggttcgtg
     8761 ccggtgttga cctctttata catgtccagc ggcgcgttct gtgaatagcg caggttatct
     8821 tcgatatagg tgttatagac gcctttcgac agcgcttctt catcaccgcc accggtccac
     8881 acgcgctgac cttttttacc gacgatgatc gcggtgccgg tatcctggca ggtcggcagc
     8941 acgcctttgg cggcgatttc ggagtttctt aagaattgca gcgccacgta cttgtcgttt
     9001 tcgctggctt ctggatcgtg aagaatagcc gcgacctgtt tctggtgtgc cgggcggagc
     9061 atgaaagaag cgtcgtgaaa ggcttgctgc gccagcagag tcagggcttc tggttccact
     9121 ttcaggattg tttcgccgtc gaagtcggca acgctaacgt aatcggaagt gagtagatag
     9181 tattcggtat tgtctttccc catcgggaaa ggtgcctggt agataaaggg tttgtttgac
     9241 atagcttcca gcctgtaact ctgtatttgt tattcgaaaa tggcgtgccg cgtagtcacg
     9301 ggcggcacgc aaagtgcatt tataagaacc cgtacatcgc ggcgaagatc cagccgaaga
     9361 cgcacgatac gctcacacca atcaaccccg gcagaataaa gctgtggttg atgacgaagc
     9421 gaccgatgtg ggtggtgccg gaacggtcaa actgaatcgc tgccagatcg ctcggataag
     9481 tcggcaggat gtaataaccg tagcaagccg gtgctgaagc cacgatgtat gccggatcaa
     9541 cgccgatcgc cagcgcgacc ggaacaatcg ccgccagcgc cgcagcctga gagtttacaa
     9601 acttggaaac cagcagcaga acaatggcat aggcccacgg atactctttc accatttcgc
     9661 ccagtacgcc ctgaatttca gacatatgcg caccgaacat ggtttcagcc atccatgcga
     9721 taccgtacac cgccacgatg gcgatcatac cggaacggaa gacttcgttt tttgagatag
     9781 acgcgggatt ggttttggtc aggataataa tcagcgcccc ggtcagcagc ataaacatct
     9841 gaataaccag taccatcgac agcggtttgc caccgaagga tggacgcagg tccgaatcag
     9901 caccaagaag tgcgactaca gcgattgccc cgaggaagat ccacattgca agccagttgc
     9961 ttttcggcag ttttttatcg agcagcgtcg cggtatcacc gtaaacatac tcacggtttt
    10021 ccggtatgga gatgaatttc tggaactctt cgtctttatc cagatcttta ccgcggaacc
    10081 agctgaagat accgatcgcc aggataccga ttaacgtcga tggaatggtg attgccagca
    10141 ggtcgaggaa ctcaagatgg cgaccatcaa aggtgacatt acccagcatc gcaaccagag
    10201 acacgactgc aaccgacacc ggactggcga taatccccat ctgtgcaccg atagaacttg
    10261 ccgccatcgg gcgttccgga cggatgttgt tcttaatggc gacgtcgtag atgatcggca
    10321 gaatggtgta aaccacatga cccgtaccgc aaagaatggt cagcgtacag gtcacaaacg
    10381 gcgcgacaat tgagacatat ttcgggttgc ggcgcagcag cttctcggca atttgcagca
    10441 tgacatcaag accgcccgaa gcttgcaagg tcgccgatgc tgccaccacc gcaatgataa
    10501 ccagcatgac atcaaccggt ggtttacctg gctgaaggtg gaagacgaag accagaatga
    10561 ccagaccgat accgcctaat aaacccagcg cgataccacc ctttctggca ccataaaaca
    10621 gacatatcag tattatgata agttggatag taaataacat gtgtgaaccc tcgcgataat
    10681 cctatttaaa tttttgctga atagatcaca gtcacgttct gttttgtatg aactgttttc
    10741 agagcctgac taaatatccg tttggtcgtt accggcttga gcaaatacct cacagtgaat
    10801 attggctgat taaagcagcg gcaaatttat gcactgtgat aagcggcttt ttcagagaga
    10861 aaaactctct ggcgatgggt ttatagtttg tactgcgcag atgttaatgt tttgtttgct
    10921 gttatcttat tgatatttat cggtctattt tttgttctca gggcagatat cattaggttt
    10981 atagattaat ctgatctacc catttgtagg taaaaataca cataatacgg gtgacataat
    11041 agttaattaa cttttgttag cgttttgaaa ttaaaaacac cgttcacctg aagagatatt
    11101 aatttttagc gatgatggag ggataattat atttgatctg gcacaagttt tactgatgaa
    11161 ggatgtaact tgtgccaggg gtattttgca ttacagtaat aattattact tacagataac
    11221 cagcgatccg ttattggcaa tattgtttca gtagtgagta gtgttctgcc tgaatacggt
    11281 aacggtaaac cggacgcccc gtgacgccat aatggatact ggtgaacaag atgtggcagt
    11341 tgaccagcca gatgaggtat ttacggcagg aaacacgcga aatgttaacc tcgttagcca
    11401 gctcgtcggt tgaaaactca tagtcctgat gcgcgtcaat ccactgacac agcgtgcgta
    11461 acgtctgcgg cgttaagcct tttggcaagc gacgaggatc ctgctcgttg gagctgctgc
    11521 cgtggattag ctgatcaagc tcggcctggt cataatactg atgtttttcc agcgccattt
    11581 tcttttgccg ccagccggtg agcgcctctt caaagcggga agcctggaag ggtttgatca
    11641 ggtaatccac gacaccgtaa tgcagcgaat ctttaatggt tgccgcatcg gctgcggagg
    11701 agatgacaat cacatcactt ttgcaacgcg cgttatgcag gacaggcagt aaatcgagcc
    11761 cgttctcttt ttgcatatag atatcgagca atatcaggtc gataggcgta tcgctattga
    11821 agataatctc tttggctttc tccagcgtcg aagccgttcc acagcattga aagcctggga
    11881 tttgtgccac gtatcggcga ttcagctccg cgaccattgc gtcgtcatcg ataattaata
    11941 cattgatcat ctgttcgacc tctccccgtc ccagggtatc tggacaaaaa attgtgtgaa
    12001 aatcccgggt tccgattcca cggcgatgct gccgccgaga ttttctacct gttgtttgac
    12061 aagtgctaaa ccgacgcctc gctcgcttcc ttttgtcgag acacctttgt caaaaatgtg
    12121 atcgattttg tcgggtgcga tccccggtcc atcatcgtta acttcacagt gcagccagcc
    12181 gtgacggtag tgcaatgtta cgctaatttc gccaccgggt tccggtccta atgcctccag
    12241 cgcgttttct atcagatttc ccagcgtggt aatcagcgtc gcgacctggt cttcactgcc
    12301 gctgtctggc aactggcttt cactgtttaa aatcagcgta tggcctaaat cggtcgcgcg
    12361 gttaatctta ctgattaaaa aaccagcgat aaccggagat ttgatcttac ccagcagaga
    12421 gccaatctct tcttgatagt tattggctgt tttgagaatg taatcttcca actgcttata
    12481 actcttcaga tgcaataatc cgagaatcac gtgcaattta ttcataaatt cgtgggatcg
    12541 ttcacgaagt gcgtcagcat agttgaccag accatcgagt cgctgcatca gtttacgtac
    12601 ttcagttttg tccctgaagg ttgaaatggc accgatgata acgccattac tgcgcaccgg
    12661 aacggtgttg atcagtaata gccggtcttt aatcgtaatc tcttcgtcgc gccgcggggt
    12721 accgtcgcgt aacacttccg agacatctac cacctgtgac cacgagtggc ttagcgtcga
    12781 cagcttctca tcgtcctgcg acttacggta attcagcaat tcttgtgcgg catcgttgat
    12841 cagcgtgact tcaccgcgat cgtccacggc aacgacgcct tctttgatgg actgcaacat
    12901 agcctggcgt tgctcaaaca gcgtggagat ttcgtagggt tccaggccga aaaggatttt
    12961 tttcagtacc ttaaccagaa tgcaggtgcc aatcagcccg accagcatgc caaataatac
    13021 cgaccagatg atgctccagc gactgtcatt gatctgttgg gtcacgcgac ttaactcaag
    13081 gccgatcgcc accacgccaa tttgtttatg attttcatcg tagatggggg taaatacgcg
    13141 taaagcctgc gccagaaaac cgcgattgat agcgacattt tcttcgccat tcagcgcttt
    13201 aaggatgtca tcacctttaa atggctgacc aatacgctgg gcttcaggat gcgagtagcg
    13261 aagactttgc atatcggtaa cgacaataaa cagcagatcg ttgcgtttgc gcacggcttc
    13321 cgcgatggcc tggattccac tctcctgtgg ttttttctgc aagccctgac ggatttccgg
    13381 cgagtcggcg agggtacgcg ctactgccag tgccttgtta gctagcccgt ctcgcgtcat
    13441 atcactgatt tgcgagaagt aaatcagatg caccaccaat agcaccgaga acagtaccgc
    13501 gctgaccatt aagatcactg tggtactcaa tttcatcgga cgtttgcgta acatgcggta
    13561 gggcaatgaa tgtctcatca gcttccttgt gtgacaaatt tcttaagcat tatctctgat
    13621 gaggcgggta attcaaaggg agtaagaatg attggcatat aggggaagag actctggcaa
    13681 cggaaactgc cagtgctgta tgaagattcc agggctatgc ttatagtgaa aaccatactg
    13741 atgagagagg gaaggtcatg gatcaggcgc tactggacag gggttatcgc tgttataccg
    13801 gcgaaaagat cgatgtctat ttcaacactg cgatatgtca gcattctggc aattgcgtac
    13861 gtggcaacgg caagttattt aatctcaaac gaaagccgtg gatcatgccg gatgaagtcg
    13921 acgtcgccac tgtggttaaa gtgattgata cgtgcccgag cggcgcgctg aaataccgtc
    13981 ataaataagc gagggtaaaa tggaaatacg cgaaggccac aataaatttt acattaatga
    14041 cgaacagggc aagcaaatcg ctgaaattgt ctttgtgccg accggagaga atttagcgat
    14101 tatcgaacat accgatgtcg atgaaagcct gaaagggcaa gggattggta aacagctggt
    14161 tgcgaaagtc gtggaaaaaa tgcgtcggga aaaacgaaaa attatcccac tatgcccatt
    14221 tgcgaaacac gaatttgata aaacgcggga gtatgatgat attcgcagtt gatgggagag
    14281 tacagagtca cgatattttt catgctctcc gcggtgtgat gcaggagagc atctgaaggg
    14341 tagggggatg cacaaagaat gggcagagag cgcgtttttt tgtcccaagt catccccttt
    14401 actgagcaaa aaaaagaata tctcctatat gagaatcatc aatcggggtt aataagtttt
    14461 gcgtccccag agcgtttaat attgatagga gtcatattat ggaaggtaaa aacaagttca
    14521 atacttatgt tgtttctttt gattatccat catcttattc ctcagtgttc ttaaggttaa
    14581 gatcattgat gtatgatatg agtttctcct ctatcgtggc tgatgaatat gggataccac
    14641 gacaactgaa tgaaaactcc ttcgcaataa cgacatcgtt agccgcaagt gaaatcgaag
    14701 atttaatcag gcttaaatgc ttagacttac cggatattga ctttgacctc aacattatga
    14761 cagttgatga ctatttccgt cagttttaca agtagccagg aaggataaga agaacataaa
    14821 aaatggcact attctctaaa atattaattt tttatgtgat tggtgtgaac atatcctttg
    14881 tcattatctg gtttatctca catgagaaaa cacatattcg tttacttagt gcatttctgg
    14941 tcggaataac ctggccaatg agtctgcctg tggcattact tttttctctc ttttaggagc
    15001 tgtagttgag tggacccgct ggttatcttg aaacgaagcg tctctcctca aaactggatt
    15061 tgcggaatga cgaacgaagc agtcgcctga ctgcttcgtt cattaaagtg aaatttattt
    15121 cagtgggcgc atcgccggga agagaataac gtcgcgaata gtatgactgt ttgtaaacag
    15181 cataatcatt cggtcgatac caatacccag accagcggtt ggcggcagac catattccag
    15241 cgcagttacg taatcttcgt catagaacat ggcttcgtcg tcacctgcag ctttagcgtt
    15301 aacctgttcc tggaaacgtt cagcctgatc ttctgcgtcg tttaattcgc taaaaccatt
    15361 accaatttca cgaccaccga tgaagaattc aaaacggtcg gtgatttccg ggttaacatc
    15421 attacggcgc gccagcggtg acacttctgc cggatattcc gtgataaagg ttggctgaat
    15481 cagatgtgct tctgccactt catcaaagat ctccgtgaca atgcgtccca acccccagct
    15541 tttctctacc gtaattccga tagattcagc taatgcttta gcagcatcaa aattatccag
    15601 atccgccata tcggtttctg gacgatattt tttgattgct tcgcgcatgg tgagtttttc
    15661 aaacggtttg ccgaaatcaa acacatgctc gccataagtg actttagtgg tacccagtac
    15721 ctcttgtgcc agagtgcgga acagtgattc cgtcagttca atcaaatcgt ggtaatccgc
    15781 atacgccatg tagagttcca tcatcgtgaa ctcaggatta tgacgaacag aaataccttc
    15841 attacggaag ttacggttga tttcgaatac ccgttcaaaa ccacctacaa ccagacgttt
    15901 cagatatagc tccggcgcga tacgcaggta catatctaaa tccagagcgt tatgatgggt
    15961 aataaacggg cgagcagatg ctccacctgg aattacctgc atcatcgggg tttctacttc
    16021 cataaagccg cgagcgacca tgaactgacg gatagcggcc agaatttttg aacggacaac
    16081 aaacgtttga cgggatttat cgttagcgat gaggtccaga taacgttgac gataacggac
    16141 ttcctgatcc tgcagaccat ggaatttatc tggtaaagga cgtagtgctt tagtcagcag
    16201 gcgcagctca gtacagtgaa tggaaagctc acccgtttgc gtcttaaaca gcgtaccgcg
    16261 ggcaccgata atgtcaccca gatcccattt tttaaactga tcgttataaa caccttctgg
    16321 caggctatct cttgcaacgt acagttgaat acggccaccg acatcctgca acgttacaaa
    16381 ggaggctttc cccataatac gacgggtcat cattcggcca gcaaccgaga cttcaatgtt
    16441 taaggattcc agttcctggt tatccttcgc atcaaactct tcgtgcaact ggtcagaggt
    16501 atggtcgcgg cgaaaatcat tgggaaacgc cacaccttgc tgacgtagtg ccgccagttt
    16561 ttcgcggcga tttctcagtt catcgttaaa atcaatagcc tcattggctc cccgtgtttc
    16621 tcgttcagac attttggttc ctctaaatcc agctttcaat ttagcgtaga taaagagaca
    16681 gatcggtaca ctgtattatc tgcctcgact attaataact cagtttatct tatcgaaact
    16741 aacgcttacc tcaagttgat atcactaata aataaccata aaaatggttt aaatgactta
    16801 ttaatctcga tttgctagtg tgataaactg aaagttaatt tattcataac caattgtttt
    16861 tacgacagtt aatcgttgct ctcctgtatc atattcgtcg gcgtgctaaa cagaaaacgg
    16921 gtagcaaagg ccaataccac gatgatcgcg acacaagcca acgtccactc tcccatctga
    16981 gaaaagaatc gctggtaagc ggcaattgcc ataccgctaa tttgcgactc tgtcgtctgc
    17041 tgtgccacga cgcctgccag ccagttggcg accgcgcctg ttgccagcat ataaataccg
    17101 gttaatacgc cagacatttt cagacgcgtg atttgcgcaa tcgccaccgg gtcaataaag
    17161 agttcagcaa agcccattag cgccagcccg gatatcatca cgcccattga cgcttgaccg
    17221 ccagccgctg catgtcgggc atcaaatgcc agcaacataa agccacaagc catcagcagt
    17281 aggccaaagg caaacttcag ccagacgcgc aatgctgaat tgccgcggct ttctggcgac
    17341 gccagccagg ccagaacaac cccagcgagc atcaccgcaa tagcattcac cgactggaat
    17401 agtgctgtag gtacttcaat attgaatgcc tgacgattca caaagcgatc gataaacaag
    17461 ctgatggtac tgccgccctg ttgtgccagt acccagaaca acgtcccgac aaacatcaac
    17521 aatacaattt gccaaagagc acggcggtgt tcggggaatt tgatcatcat gcgggcaatg
    17581 atttgtgctg caatgaggca aacgatcgcc agcaaatatc cggaccagtc gttctccagc
    17641 agcagggtaa aaaatactgg ggctaaacag agcatcacca ctaaccagct ccatactggt
    17701 aaggcaaatt tgacgctagt gagcgctttt ttatccatac tacgcgtgga ctggaaatga
    17761 cgatgaccac ttaagaaaat caacaaaccg ataaacatgc cgcctcccgc aagggcaaag
    17821 ccaacatgcc atccatacca ctgagcagcc aggccgcagg cgatgggcgc ggcgatagaa
    17881 ccgatattgc ccgcagcata tagcagcgaa aaaccgccat cacgtcgatg atcgttctcg
    17941 tcgtagagct cgccaagcaa acagctgatg tttgatttga ataaaccgta gccacaaata
    18001 atgattgcca gcgccagata caggctaaag gttgaatttg tatcaatgcc cagcaccaca
    18061 tggccaaggg tcattaacag cgcgccggca atcactgcag tgcggttgcc gagcaggcgg
    18121 tcggcaagcc agccgccgag aataggggta acgtaaacca gagaagcata tgcgctgaac
    18181 aggctgatgg catggttatc atcaaaacca agctgatggg tgagatagag aatgagtaag
    18241 gcacgcatgc cgtaaaaact gaagtactcc cagatttgga tcgccacgat atagtatatc
    18301 gcgcgcggct gtgagggtgt tttcatgtgt tctccttatg agcaaaaaag ggaagtggca
    18361 agccacttcc cttgtacgag ctaattattt tttgctttct tctttcaata ccttaacggt
    18421 atagcggcca tcagcctgac ggtatgcacc gtgaatatcg gtttcaaagc ccggatagtg
    18481 agcgccgatt tcacacagca tctgcaggaa ctccagaacc ggacggcttt cttcggtgat
    18541 catttcaccc ggcattacca gaggaactcc cggcggatac ggaaggatca tattggcgtt
    18601 aatacgacct accatttcgt cgaggtaaac ttcttcggtc ataccgtgca gctctttctg
    18661 gaacgcagca tacggagtca ttaccatcgt cggcagaact tcaaatgcgc gatacatcag
    18721 atccggcaga ttgtggtgaa caatcagttt gtggatattc tgagccagtt cctgaatacg
    18781 catgttttca tagaattcag gatcttcacg atacagagac ggcagcatgt ttttcacacg
    18841 caggttcaga tcgaacgcac gtttgaagtc agtcagagca cgcagcaggc tcagtgcttt
    18901 ggtcttatcg ataccgatgc tgaacaggaa cagcaggtta tacggaccgg ttttctcaac
    18961 aacgatgcca tgttcgtcga ggtatttcgc cacgatgctg gccggaatac cgaagtcgct
    19021 catggtgccg tctttttcca tccccggagt cagcagggtg actttgatcg ggtcaagata
    19081 catgtgctcg ttatcgatgt ttttgaagcc gtgccaggtg ctgtcagaac gcagcggcca
    19141 gcattcagtc gtatcgatat gatccggctg ccatacatca aagaaccagc catcagattc
    19201 cgttctcaga cgtttgatct ctttacggaa tttgatcgca cgttcaatgg aaccgttgat
    19261 cagacgctta cctgcattgc ctttcatcat cgccgcagcg gtttcagtgg acgctacgat
    19321 accgtagtgc ggagaagtgg tggtgtgcat catgtaggct tcgttaaagg tttcttcgtt
    19381 tacgtcacct ttaacgtgga tcatggaagc ctgagagaac gccgccagca gtttgtgagt
    19441 agactgggtt tcgtaaatca ctttcccttc tacacggcca ccgctcatac cgcatttacc
    19501 ttcgtaaatc ggtgagaagt tggtgtaagg cacccacgcg gagtcaaagt ggatggattt
    19561 cacatccagt gttttcttga tgaagtcggt gttgtacagc agaccatcat aggtagagtt
    19621 ggtaattaca gcatgtaccg gccaggttgc gtttggtgtt tctttcacgc gcttagcaat
    19681 ggtagcgtgc tggaattcac tctgtgggat accaccaaga ataccgtaag cgttacgggt
    19741 cgggcggaaa tagattggcg taacatcgct catcatcatc aggtgggtca gcgatttgtg
    19801 gcagttacgg tcaatcagaa tggtgctgcc tgccggagca gagtacatac caacaatttt
    19861 gttcgcagtg gaagtaccgt tggtcaccat gtagctgcgg tctgcgttaa agacgcgagc
    19921 gatatactgt tctgcttctt tgtgtggacc actgtgatcc agcagagaac ccagttcaga
    19981 tactgaaatg gaaatatcag atttcatggt gttcggacca aagaaatcat agaacaggct
    20041 acctaccggg cttttctgga atgcagtacc gcccatgtga ccaggagtac agaaagtata
    20101 tttaccttca cgaacatatt taaacagtgc tttggtcagc ggaggcagaa tagtgttgat
    20161 atattcgtca gtggtctgct tgatcttgtt agcaatatca tcagcagcac ccagcgcata
    20221 ttcaaagaag ctaatctgta aacgcaggtc gttcaggctt acatcgagag tggaatacgt
    20281 attagcgaac gcgtacaacg gcaggttctc gttcattttg ctaatttctt cgcacagctc
    20341 gagattatat ttatcccagt caaaaataac gccgcacaga cgcgcattgt tttcgatcag
    20401 ttttaataag tcgtcacggt cgttcgggta aacaatctgg aagttcagac gttcaagcgc
    20461 gcgatgaagt tcacggatgg gttcttcttt aaaataaacc cccatgtgat tcaatattgc
    20521 aataacgttc atagtcatat ctccaggtaa aaaaggcccc tcccaacaca tgggacaaaa
    20581 tgaaaggagg agcctcggaa aatactttta attaatgtgc gttagacgct gtgtggttat
    20641 ccattgagtg gctctggcgc tcgtgcattt tgcgagcgta gaacatcagg ataatcaggc
    20701 tgacgatgaa ggtacctgcc agctcgaagg agcttgcgcc catcagcgcg atgaagcaga
    20761 acacgcaacc cagtacagag cagatcaggc tgacaaagtt gcggatgtta acgccttcaa
    20821 aacgaatcag gtcaacgcaa gagtagaagt acggcagcat agtcagcagt actgcgatac
    20881 cggtcagttc accgaacagg tcagatgctt taccaccggc agagttcatc agagtgatga
    20941 ggatcatcag ggcagtcatt ttcactgcag ccagcagcag accttttttc ggaataccgt
    21001 tgctgtcgac ttcaccataa actttcggga agttaccgtc gttagcggca cgtacacctg
    21061 cctggcctac caacatcatc caggagccca gagaagtcag gcacgcaaag gcggtgaatg
    21121 cagaaaccag cggcgcagcc cagttaccga ggatagttga agcactgatt gcaaacggag
    21181 caccggaagc tgccattaca gaagacggat acataccgga aagcacctga gtcgcagcga
    21241 tataaacaat acctgctaaa ccagtaccca gcatggttgc cagcggaacg gtacgtttcg
    21301 ggtttttaac cataccagta cttactgctg cggattcaac acccacgaag gcccacaggc
    21361 agagcagaat acttttaatg atcgcatgac catcagtggt atccgcagta ttccagttag
    21421 ctgcataagt tgctgcatca aaccaatgcc agccaacaat agcagtcatc accacaggaa
    21481 taagaaccag caccagacca atagtggtta aacggcttac ccaggtaccg ccgagcatat
    21541 ttacaaaggt aaatacccag acgatagcaa tacaggcgat acccgccgga acaggatcat
    21601 ttaatactgg gaagaaggtg gaaagataag atacagcggt aataccaatt gccaggttac
    21661 caatccagtt agcatggtaa taaagaacac ctgtctgaaa accaaatgca ggagaaattt
    21721 ctccggcata cgcaattggg ccaccttgtt gcgggttttt tgttgccagt cgggcatata
    21781 catatgccag cgacattgca ccaataatag agataatcca accccagata gcaataccac
    21841 cgatacttgc taggttcgca ggtaataatg caataccgct ccccatcata ttaccggcaa
    21901 caacaccggt acaggcaaat agcccgatct tcttggcaga actcatgctc ttctcctaat
    21961 ttcatttttg aatttggagt ccgggtcatg atgtataact atttcctgac cagaccaaac
    22021 tggcgctaag attactcacg aaaaaaggat taatcctaaa gattaggtga aataacacaa
    22081 aagtttctgt aagtgagaac ttgaggtttt ttattaacac atcaggatcg caagttgata
    22141 tcatgaaaag ataaacattc aatatttaca atgtgttatg tgacatcttc tggttaaatt
    22201 tatgtaataa aaatcatgcg gcaaataaat tgccgcaaca tattatacca acgggaacat
    22261 acaaaaactc aacaacaaat attcccgagc atgaatcaat ccgggagtta cttattctga
    22321 agcgagaaat ttgtcgagat aaggtacaac ataaggaaca gaagtctgga atataccatt
    22381 ttcaatccag taaagggtgt ttgcccctgg gcgtaaatta aaggcggtga gatatgcatc
    22441 agctgcttcc cggttcatcc ccttcatttc ataaaccttg ccaagcaaca cataatttag
    22501 ccaggacatt tcaagatcaa tgccagtatt tatcgcctga taagactcat ctgttttacc
    22561 ttttaccagg gcactgaccg cttttatttg atatataatg gacaagttgt tcaattccgg
    22621 tagtgtaaca atgttatcta tttctgtgtt cagtgctgct aattgttttt cgtctaaagg
    22681 atgttgagaa tggcgcacga tatcaactaa tgctttttct gctctcgcgt aggtaaattc
    22741 tggggatgat tgaacaatct cacctaataa ttcactggca cgatccaatg atttatcatc
    22801 accatgcagt aaataatcat gtgcctgata aaaattagtt aataacgcac cacgatgcgg
    22861 caaaatttgc tggagcatct cctgcattcg ttgtggccac ggttggttta acgcttttga
    22921 taaactctgg agcaaatcat tttgaatcgc cagctgatta ccgttagtga tgacataacg
    22981 tttatccagc atggttgaac catctgcatt gtctaccaat tttaccgaca taaagcattg
    23041 ttgagcacgg tattggcgct gattaacaaa cgcaatagat aatgttttac cggaactgct
    23101 cggttcatca atgttgtagt tgattttgtc atgcaccata aaggtggaga aggtgttaag
    23161 tgatgtcgcc accaaatcac ccacgcctat cgcgtaagag agctgatacg gagaactcca
    23221 gctgttacaa ctcttattaa ccatattaat gtcaatatcg cgtggattga gcaaaatgcg
    23281 cgatttactc ataggaagac gtgtttcaag acttgaaaac gctaccagtg cgacacagat
    23341 acctaacgac aacaggaaaa aaaaccatac ccaaaaggta gtgaatcgtt tgcttttaac
    23401 tggggattgt tcaggtggcg ttgtggtgtt ttgaatgtta agactgtggg agggagaatc
    23461 tgtggcagga accgcctctg gtataggggg aggcgaagat agcattattt cctctccctc
    23521 ttcttcgctg taccagataa ccggcaccat taatttatag ccgcgctttg gtacagtagc
    23581 gatatagaca ggactatctt catcattatc ttttaatgac ttacgtagtt ctgagatact
    23641 ctgcgtcaca acgtgattgg tgacaatact tctcttccag acattatcga taagttcatc
    23701 cctgctaagt acttcgccac tgtgttgagc aaagaaaacc agaagatcga ttaatctcgg
    23761 ctcaagggta agttgacgcc cattgcggct aatttggttt atggacggag taacaagcca
    23821 ttcgccaacg cgaactacag gttgttgcat aatagaaact cattcgaaaa gggaatgatg
    23881 caatgataat tgccataacc tattttttcc atctatagat gggtttattt acatattatt
    23941 ggtgaatgta ggacgttatt tttacccgcc ataaacttct tgattacata gtattacgaa
    24001 aggattttac tgagaaccag aagtaatttt ccttaccatc aaaattcatc atctttgcca
    24061 aagaaaaatg ttcagaaaat aatccatgaa aaattgtccg gagcgcttac tattttaatg
    24121 gattgttagt ctttgcatga gcaaacggac tgatacattt ctctttgttc ttattcagaa
    24181 aatctcatca gttggcgttc tgcccgatgt tgtgctttat gggtgcgtta cgctcctgat
    24241 gacgtcattt gacgttcaac agcatcacgg ggccgcacga catttcacgt cagtcagcgc
    24301 tatagctcag aaacaaattt tcccgaattg gaatatgccc gtaaattgct ggtgatgtgg
    24361 ggagaatctg gttgagttcg gtagaattga tttggcggca ggacgcttaa atcgtggcgt
    24421 cctgaaacga aaaacggacc tccgtggagg tccgtttata tgaatttggt gccggactcg
    24481 gaatcgaaaa tttcttttat catattgata ttaatataat ttatttggtt tgattaaaaa
    24541 tgcaccctca attatacccg cagtcccttt tgatatcgaa agggaatcac actcgggatc
    24601 aggctgagag cacagaagga aagtacctgg ctattatatc gttaattctg tcaatcagtt
    24661 ccagcctttt gaaagtgatg tgtgcagatc ccttcttaaa gtatttaatg atgaacatct
    24721 cgtcttcata gcgctcttta ccctgaacgg actggatgtg accatccaga cgaatagtga
    24781 tgttttcccg gttgtcgggg atcggtttgc cgctgaacag atgtagcatt ctttccaggt
    24841 cgacgagtcg gtcagtctgt tgcccggtga taagatgaaa cccccaccgg tcccacctca
    24901 ctaggttgtt gacgataatt ttactgccaa atttgcaggg gcaattggtt ttgtaattcc
    24961 agttcagtcc cctgaagacg ttgatcaccc ctcgctcaaa cacctcatcc ttattctggt
    25021 gtagttgttc aaatgtgctc agaatgttcg cttcactgat ttccggaaaa ttatcgtgct
    25081 ccagtgaccg gtaccatgta tcacgggcct gtgcatccat taaggaaagc atccctgaac
    25141 gttgcatcag gtcacgccag atttcgcgat cgagattgcg tgtgatggct ttcattgcgg
    25201 tttccggttt ctccatcagc cagcagccgc agcgaaaatc ctgtttcatg gcccagtccg
    25261 gggcggcttt accgccaata ctttctgtca ttgctgatat ttcagccagt tgccggataa
    25321 gtgtgtcgat ttgcagcagt gtactctttc gtacagccag aatgtggcgg atactggttg
    25381 agcagataac gtccgtatgc ccggtgacca cttccggacg ggagattgtg atgtctgaca
    25441 ttatcggttt cccttttggt ttataaacga aaacgccagc cctgttcagg ctgacgtcag
    25501 tggaatgaag tatggtgagt gatatttacc ggttctggtg cagaagctgc agatgacgaa
    25561 gagtacggac atcctcccgg gcctgttcga tacagggcaa cagtgctacc gaatccgttt
    25621 tatcctcatc gttgtcaaag ataattcccg attcgcagtc gatattgtcc tgcagccacg
    25681 caatgaggat atccagcgct gtttcagtgg ttaatggcat cacgctctgt atcactgtat
    25741 tgtcttccgg caactgatga gggctggcag aagtcgccgg tgtttcggta gcgtcacgtt
    25801 cattcaccac gcggagtgaa taaccgtttt cagcgacttt gttaatcagt tcagcgagct
    25861 caataccatc gacgtcaacg acaatgcgcc ccatgttcag cgcctgcgca ttaatgctgt
    25921 cggcttccag cgtcagggcc agtttcattg tttcgccccc ggatgtttac ccagggtaat
    25981 gttatttacc gttctgtagt tgtcgcgtgt catcaggccg gttgcccggc gtgcccggag
    26041 gatatcaata ctgtttatta actgagaacg ggtacaggcg ctgaatcccg gctggtcggt
    26101 acgtaccagt gcgtattttt caacgagaaa gttcaccgca tcgcacagtg aaatgcctgc
    26161 ctcaatatgc tgctcaatca cacgttcatc agcgaacggt gtgtcattca gcgtaaggcc
    26221 gtagtgctga tccagcagtc gggtgagcag tgtctgccag atttcaaccg gagacgggcg
    26281 tgaactggcc gcctgcccgg gtaatacagg taatgttttc atactgaaga ttttcctgat
    26341 atgcagagat gaaaaatgga aaagtggcgt ggtgaaagca ccaggccgga gcagaaggtt
    26401 attctggaga gttacttttt cgtttcgggc gtcggataaa cagccatata aacgtagcca
    26461 caactgccga gggtgtcggc ttcgcaggtc agcccttttg catacagcgt gacggtatgc
    26521 tgatggcggg gattcagttc accgctggtg agcatgagtt ccagttgttt catcagcagc
    26581 ggaaaggcct ggtccaggtg gtacgaatcc gcgtcgctga accgccctct gataccggcg
    26641 cggtcggcaa ggtaatgcaa ccggttgcct tcctgtacca gacgggcacc aaaacagggc
    26701 gtcacggtgc agggcagccc ccaccaggtg cggtcgttat tgtcgtcggg aagcgttgtc
    26761 ccggggagtg tgtctgacac gataaaatcc tcacagaaaa ttggttaaga atggtccggt
    26821 ggtggcgata attctgctct tctgaatgcc cttccaga
//
LOCUS       DAPHCV010000071        26489 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.071, whole genome shotgun sequence.
ACCESSION   DAPHCV010000071 DAPHCV010000000
VERSION     DAPHCV010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.071"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            43..1512
                     /gene="gspA"
                     /locus_tag="RH033_002904"
     CDS             43..1512
                     /gene="gspA"
                     /locus_tag="RH033_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HDV2992058.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSSQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSTGRLMEH
                     ELKASILEGQNIDQPDLLLTARVLKRIALLCRGDRRKLALAGETISLLQQAEQTSVFT
                     AKHWRMIYRVLGDNRPRKMQLAVVMSGTIIALTCGWLLLSSFTDPFPVPAWLVPVTPV
                     VKQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLACKSGNASLQTL
                     VDQNLPWIASLKVGDKKLPVVVVRVGDATVDVLVGQQTWTLTHQWFESVWTGDYLLLW
                     KMSPEGESTITRDSSEEEILWLETMLNRALHISTEPSAEWRPLLVEKIKQFQKSHHLK
                     TDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            1514..1933
                     /gene="gspB"
                     /locus_tag="RH033_002905"
     CDS             1514..1933
                     /gene="gspB"
                     /locus_tag="RH033_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HDV2992059.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPDVA
                     VENAPSSSEDEENTVEESDEKAGLRERVKNALNELER"
     gene            2171..2482
                     /gene="rpsJ"
                     /locus_tag="RH033_002906"
     CDS             2171..2482
                     /gene="rpsJ"
                     /locus_tag="RH033_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDV2992060.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2515..3144
                     /gene="rplC"
                     /locus_tag="RH033_002907"
     CDS             2515..3144
                     /gene="rplC"
                     /locus_tag="RH033_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDV2992061.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3155..3760
                     /gene="rplD"
                     /locus_tag="RH033_002908"
     CDS             3155..3760
                     /gene="rplD"
                     /locus_tag="RH033_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDV2992062.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3757..4059
                     /gene="rplW"
                     /locus_tag="RH033_002909"
     CDS             3757..4059
                     /gene="rplW"
                     /locus_tag="RH033_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDV2992063.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4077..4898
                     /gene="rplB"
                     /locus_tag="RH033_002910"
     CDS             4077..4898
                     /gene="rplB"
                     /locus_tag="RH033_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDV2992064.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4915..5193
                     /gene="rpsS"
                     /locus_tag="RH033_002911"
     CDS             4915..5193
                     /gene="rpsS"
                     /locus_tag="RH033_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDV2992065.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5208..5540
                     /gene="rplV"
                     /locus_tag="RH033_002912"
     CDS             5208..5540
                     /gene="rplV"
                     /locus_tag="RH033_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDV2992066.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5558..6259
                     /gene="rpsC"
                     /locus_tag="RH033_002913"
     CDS             5558..6259
                     /gene="rpsC"
                     /locus_tag="RH033_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDV2992067.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6272..6682
                     /gene="rplP"
                     /locus_tag="RH033_002914"
     CDS             6272..6682
                     /gene="rplP"
                     /locus_tag="RH033_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDV2992068.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6682..6873
                     /gene="rpmC"
                     /locus_tag="RH033_002915"
     CDS             6682..6873
                     /gene="rpmC"
                     /locus_tag="RH033_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDV2992069.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6873..7127
                     /gene="rpsQ"
                     /locus_tag="RH033_002916"
     CDS             6873..7127
                     /gene="rpsQ"
                     /locus_tag="RH033_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDV2992070.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7292..7663
                     /gene="rplN"
                     /locus_tag="RH033_002917"
     CDS             7292..7663
                     /gene="rplN"
                     /locus_tag="RH033_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDV2992071.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7674..7988
                     /gene="rplX"
                     /locus_tag="RH033_002918"
     CDS             7674..7988
                     /gene="rplX"
                     /locus_tag="RH033_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDV2992072.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            8003..8542
                     /gene="rplE"
                     /locus_tag="RH033_002919"
     CDS             8003..8542
                     /gene="rplE"
                     /locus_tag="RH033_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDV2992073.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8557..8862
                     /gene="rpsN"
                     /locus_tag="RH033_002920"
     CDS             8557..8862
                     /gene="rpsN"
                     /locus_tag="RH033_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDV2992074.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8896..9288
                     /gene="rpsH"
                     /locus_tag="RH033_002921"
     CDS             8896..9288
                     /gene="rpsH"
                     /locus_tag="RH033_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDV2992075.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9301..9834
                     /gene="rplF"
                     /locus_tag="RH033_002922"
     CDS             9301..9834
                     /gene="rplF"
                     /locus_tag="RH033_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDV2992076.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9844..10197
                     /gene="rplR"
                     /locus_tag="RH033_002923"
     CDS             9844..10197
                     /gene="rplR"
                     /locus_tag="RH033_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDV2992077.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10212..10715
                     /gene="rpsE"
                     /locus_tag="RH033_002924"
     CDS             10212..10715
                     /gene="rpsE"
                     /locus_tag="RH033_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDV2992078.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10719..10898
                     /gene="rpmD"
                     /locus_tag="RH033_002925"
     CDS             10719..10898
                     /gene="rpmD"
                     /locus_tag="RH033_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDV2992079.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10902..11336
                     /gene="rplO"
                     /locus_tag="RH033_002926"
     CDS             10902..11336
                     /gene="rplO"
                     /locus_tag="RH033_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDV2992080.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11344..12675
                     /gene="secY"
                     /locus_tag="RH033_002927"
     CDS             11344..12675
                     /gene="secY"
                     /locus_tag="RH033_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDV2992081.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12707..12823
                     /gene="rpmJ"
                     /locus_tag="RH033_002928"
     CDS             12707..12823
                     /gene="rpmJ"
                     /locus_tag="RH033_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDV2992082.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12970..13326
                     /gene="rpsM"
                     /locus_tag="RH033_002929"
     CDS             12970..13326
                     /gene="rpsM"
                     /locus_tag="RH033_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDV2992083.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13343..13732
                     /gene="rpsK"
                     /locus_tag="RH033_002930"
     CDS             13343..13732
                     /gene="rpsK"
                     /locus_tag="RH033_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDV2992084.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13766..14386
                     /gene="rpsD"
                     /locus_tag="RH033_002931"
     CDS             13766..14386
                     /gene="rpsD"
                     /locus_tag="RH033_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDV2992085.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14412..15401
                     /gene="rpoA"
                     /locus_tag="RH033_002932"
     CDS             14412..15401
                     /gene="rpoA"
                     /locus_tag="RH033_002932"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDV2992086.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15442..15825
                     /gene="rplQ"
                     /locus_tag="RH033_002933"
     CDS             15442..15825
                     /gene="rplQ"
                     /locus_tag="RH033_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDV2992087.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15932..16300
                     /locus_tag="RH033_002934"
     CDS             15932..16300
                     /locus_tag="RH033_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HDV2992088.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILADDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            16311..16736
                     /gene="zntR"
                     /locus_tag="RH033_002935"
     CDS             16311..16736
                     /gene="zntR"
                     /locus_tag="RH033_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HDV2992089.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKAIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16792..17010
                     /gene="arfA"
                     /locus_tag="RH033_002936"
     CDS             16792..17010
                     /gene="arfA"
                     /locus_tag="RH033_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HDV2992090.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVERNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(17007..17417)
                     /gene="mscL"
                     /locus_tag="RH033_002937"
     CDS             complement(17007..17417)
                     /gene="mscL"
                     /locus_tag="RH033_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDV2992091.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPTPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17547..18923)
                     /gene="trkA"
                     /locus_tag="RH033_002938"
     CDS             complement(17547..18923)
                     /gene="trkA"
                     /locus_tag="RH033_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HDV2992092.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18945..20234)
                     /gene="rsmB"
                     /locus_tag="RH033_002939"
     CDS             complement(18945..20234)
                     /gene="rsmB"
                     /locus_tag="RH033_002939"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDV2992093.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDACYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     VLPGFEEGWVTVQDASAQGCMAWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(20280..21227)
                     /gene="fmt"
                     /locus_tag="RH033_002940"
     CDS             complement(20280..21227)
                     /gene="fmt"
                     /locus_tag="RH033_002940"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDV2992094.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSDHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(21242..21751)
                     /gene="def"
                     /locus_tag="RH033_002941"
     CDS             complement(21242..21751)
                     /gene="def"
                     /locus_tag="RH033_002941"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDV2992095.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21881..23005
                     /gene="dprA"
                     /locus_tag="RH033_002942"
     CDS             21881..23005
                     /gene="dprA"
                     /locus_tag="RH033_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDV2992096.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLSWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPLGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLSDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22977..23450
                     /locus_tag="RH033_002943"
     CDS             22977..23450
                     /locus_tag="RH033_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HDV2992097.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23479..24021
                     /locus_tag="RH033_002944"
     CDS             23479..24021
                     /locus_tag="RH033_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HDV2992098.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            24026..24598
                     /gene="tsaC"
                     /locus_tag="RH033_002945"
     CDS             24026..24598
                     /gene="tsaC"
                     /locus_tag="RH033_002945"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HDV2992099.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24603..25421
                     /gene="aroE"
                     /locus_tag="RH033_002946"
     CDS             24603..25421
                     /gene="aroE"
                     /locus_tag="RH033_002946"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDV2992100.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            25418..25675
                     /locus_tag="RH033_002947"
     CDS             25418..25675
                     /locus_tag="RH033_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HDV2992101.1"
                     /translation="MNQAIQFPDREEWDENKQCVCFPALVNGMKLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25651..26205)
                     /locus_tag="RH033_002948"
     CDS             complement(25651..26205)
                     /locus_tag="RH033_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDV2992102.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKWLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6748 a         6258 c         6919 g         6564 t
ORIGIN      
        1 ttttatgatt aaggataatc aataattaaa acaggaagtt ctatgtctac gagaagagaa
       61 gttattcttt cctggttgcg tgagaaacgt cagacctggc gtctgtgcta tttgttgggt
      121 gaggctggaa gtggaaaaac ctggctggcg cagcaactac aaaaagataa acatcgccgt
      181 gtgattactt taagcctcgt tgtttcctcg caaggtaagg ccgcatggat cgttaccgac
      241 gataatgcgg ctgaacaggg ctgccgtgac agcgcctgga cgcgagatga gatggcgggt
      301 caattactgc atgcgcttca tcgaactgat agtcgctgcc ctcttattat tattgaaaat
      361 gctcacctga atcaccgccg catacttgat gatctccaac gcgcgatatc tcttattcct
      421 gacggtcaat ttttgctgat cggcagaccc gatcgaaaag tcgaacgtga ttttaaaaaa
      481 cagggcattg aacttgtctc aacaggacgt ctgatggagc acgagcttaa ggcaagcatc
      541 cttgaaggtc agaatattga ccagcccgat ctccttttaa ctgccagagt tctgaaacgg
      601 atagctttat tatgtcgggg agatcgcaga aagctggcgc ttgccggcga aacaattagc
      661 ttattacagc aagccgagca gaccagcgtt ttcactgcaa aacattggcg aatgatttac
      721 cgcgtattag gtgataaccg cccccggaaa atgcagcttg ccgtggtgat gagtggaacc
      781 attatcgcac ttacctgtgg ttggctactg ctgtcctctt tcacagatcc gttccccgtt
      841 cctgcgtggc tggtacccgt aacgccagta gttaaacaag atatgactaa ggatattgct
      901 catgtagtga tgcgcgatag tgaggcgcta agcgtgttat acggcgtatg ggggtacgag
      961 gtcccagctg attcggcctg gtgcgatcag gcagtaagag cggggctggc atgtaaaagt
     1021 gggaatgctt ctttacaaac attggtagat cagaatttgc cctggatcgc atcgcttaaa
     1081 gtcggggaca aaaaattgcc tgttgtggtc gttcgcgtcg gtgatgctac cgttgatgtc
     1141 ctcgtagggc agcaaacctg gacgctgaca catcaatggt ttgagtcggt atggacgggt
     1201 gattatcttc tgttatggaa aatgtcgccg gaaggggaga gtaccattac gcgtgatagc
     1261 agcgaggaag agattctctg gctggaaacg atgttaaacc gcgcattgca tatttcgaca
     1321 gaaccttcgg cagagtggcg tcccttgttg gtagaaaaaa taaagcaatt tcaaaaaagc
     1381 caccacttga aaactgacgg tgttgttggc ttttcaacgt tagtacatct ctggcaggtg
     1441 gcaggggaga gtgcctactt atatcgggat gaagcaaata tttccccgga aacaacagtg
     1501 aaggggaaat gacatgtttg aattctatat cgccgcgcgt gagcagaaag aaacaggtca
     1561 ccctggtatc ttctcccgac aaaaacacag caccataata tatgtcattt gtttattgct
     1621 gatttgcctg tggtttgcag gaatggtgtt ggttggtggg tatgccaggc agctatgggc
     1681 gctttggata gtaaaagcag aagtcactgt agaggctgaa acgcccgctt ttaaacaatc
     1741 aacacaacac tatttcttca aaaaacagcc actccccgtc gtcgaatccg tcgaggagga
     1801 agatgaccca gacgtcgcgg ttgagaatgc tccctcttct tctgaagacg aagaaaatac
     1861 tgtagaagaa agcgacgaga aagccggttt aagagagcgt gtcaaaaatg cgctgaacga
     1921 acttgagaga taacccgaag gctgtttact ttactagcaa tacgcttgcg ttcggtggtt
     1981 aagtatgtat aatgcgcggg cttgtcgtag ttgacagcag gttcaatctg aacctcagtt
     2041 agccgaattt ggctacctaa caatgctccc aatcggggag ctacgtaaga acggttacac
     2101 tctcccatca atcgtaatgg gtctgaggag taatcatttt cgtttataaa ataattggag
     2161 ctctggtctc atgcagaacc aaagaatccg tatccgcctg aaagcgtttg atcatcgtct
     2221 gatcgatcaa gcaaccgcgg aaatcgtcga gactgccaag cgcactggtg cgcaggtccg
     2281 tggtccgatc ccgctgccga cacgcaaaga gcgcttcact gttctgatct ccccgcacgt
     2341 caacaaagac gcgcgcgatc agtacgaaat ccgtactcac ttgcgtctgg ttgacatcgt
     2401 tgagccaacc gagaaaaccg ttgatgctct gatgcgtctg gatctggctg ccggtgtaga
     2461 cgtgcagatc agcctgggtt aatcaggtca ttgagcgatt gagaggttga aacaatgatt
     2521 ggtttagtcg gtaaaaaagt gggtatgacc cgtatcttca cagaagacgg cgtttctatc
     2581 ccagtaaccg taatcgaagt tgaagcaaac cgcgttactc aggttaaaga cctggctaac
     2641 gatggctacc gtgctattca ggtaaccacc ggtgctaaaa aagctaaccg tgtgaccaag
     2701 cctgaagctg gccacttcgc taaagctggc gtagaagctg gccgtggtct gtgggaattc
     2761 cgcctggctg aaggcgaaga gttcactgta ggtcagagca ttagcgttga actgtttgct
     2821 gacgttaaaa aagttgacgt aactggcacc tctaaaggta aaggtttcgc aggtaccgtt
     2881 aagcgctgga acttccgtac ccaggacgct actcacggta actccttgtc tcaccgcgtt
     2941 ccgggttcta tcggtcagaa ccagactccg ggcaaagtgt tcaaaggcaa gaaaatggca
     3001 ggtcagatgg gtaacgaacg tgtaaccgtt cagagccttg acgtagtacg cgttgacgct
     3061 gagcgcaacc tgctgctggt taaaggtgct gtcccgggtg caaccggtag cgacctgatc
     3121 gttaaaccag ctgtgaaggc gtaaggagat agcaatggaa ttagtattga aagacgcgca
     3181 gagcgcgctg actgtttccg aaactacctt cggtcgtgat ttcaacgaag cgctggttca
     3241 ccaggttgtt gttgcttatg cagctggtgc tcgtcagggt actcgtgctc agaagactcg
     3301 tgctgaagta actggttccg gtaaaaaacc gtggcgccag aaaggcaccg gccgtgcgcg
     3361 ttctggttct atcaagagcc cgatctggcg ttctggtggc gtgacctttg ctgctcgtcc
     3421 gcaggaccac agtcaaaaag ttaacaagaa gatgtaccgc ggcgcgctga aaagcatcct
     3481 gtccgaactg gtacgtcagg atcgtctgat cgttgtcgag aagttctctg tagaagcgcc
     3541 gaaaactaag ctgctggcac agaaactgaa agacatggct ctggaagatg tgctgatcat
     3601 caccggtgag ctggacgaaa acctgttcct ggctgcgcgc aacctgcaca aggttgacgt
     3661 acgcgatgca actggtatcg acccggttag cctgatcgcc ttcgacaaag tcgtaatgac
     3721 tgctgatgct gttaagcaag ttgaggagat gctggcatga ttcgtgaaga acgtctgctg
     3781 aaggtgctgc gtgcaccgca cgtttctgaa aaagcgtcta ctgcgatgga aaaatctaac
     3841 accatcgtac tcaaagttgc taaagacgcg accaaagcag aaatcaaagc tgctgtgcag
     3901 aaactgtttg aagtcgaagt cgaagtcgtt aacaccctgg tagttaaagg gaaagttaaa
     3961 cgtcacggac agcgtatcgg tcgtcgtagc gactggaaaa aagcttacgt caccctgaaa
     4021 gaaggccaga atctggactt cgttggcggc gctgagtaag tcggaggagt aatacaatgg
     4081 cagttgttaa atgtaaaccg acatctccgg gtcgtcgcca cgttgttaaa gtggttaacc
     4141 ctgagctgca caagggcaaa ccttttgctc cgttgctgga aaaaaacagc aaatccggtg
     4201 gtcgtaacaa caatggccgt atcaccactc gtcatatcgg tggtggccac aagcaggctt
     4261 accgtattgt tgacttcaaa cgcaacaaag acggtatccc ggcagttgtt gaacgtcttg
     4321 agtacgatcc gaaccgttcc gcgaacatcg cgctggttct gtacaaagac ggtgaacgcc
     4381 gttacatcct ggcccctaaa ggcctgaaag ctggcgacca gattcagtct ggcgttgatg
     4441 ctgcaatcaa accaggtaac accctgccga tgcgcaacat cccggttggt tctactgttc
     4501 ataacgtaga aatgaaacca ggtaaaggcg gtcagctggc acgttccgct ggtacttacg
     4561 ttcagatcgt tgctcgtgat ggtgcttatg tcaccctgcg tctgcgttct ggtgaaatgc
     4621 gtaaagtaga agcagactgc cgtgcaactc tgggcgaagt tggcaatgct gagcatatgc
     4681 tgcgcgttct gggtaaagca ggtgctgcac gctggcgtgg tgttcgtccg accgttcgcg
     4741 gtaccgcgat gaacccggta gaccacccac atggtggtgg tgaaggtcgt aactttggta
     4801 agcacccggt aactccgtgg ggcgttcaga ccaaaggtaa gaagacccgc agcaacaagc
     4861 gtactgataa attcatcgta cgtcgccgta gcaaataatt ttagaggata agccatgcca
     4921 cgttctctca agaaaggtcc ttttattgac ctgcacttgc tgaagaaggt agagaaagcg
     4981 gtggaaagcg gagacaagaa gcccctgcgc acttggtccc gtcgttcaac gatctttcct
     5041 aacatgatcg gtttgaccat cgctgtccat aatggtcgtc agcacgttcc ggtatttgta
     5101 accgacgaaa tggtcggtca caaactgggt gaattcgcac cgactcgtac ttatcgcggc
     5161 cacgctgctg ataaaaaagc gaagaagaaa taaggtagga ggaagagatg gaaactatcg
     5221 ctaaacatcg ccatgctcgt tcttctgctc agaaggttcg ccttgttgct gacctgattc
     5281 gcggtaagaa agtgtcgcag gctctggata ttttgaccta caccaacaag aaagcggctg
     5341 tactggtcaa gaaagttctg gaatctgcca ttgctaacgc tgaacacaac gatggcgctg
     5401 acattgacga tctgaaagtt acgaaaattt tcgtagacga aggcccgagc atgaagcgca
     5461 ttatgccgcg tgcaaaaggt cgtgcagatc gcatcctgaa gcgcaccagc cacatcactg
     5521 tggttgtgtc cgatcgctga gactctggag actagcaatg ggtcagaaag tacatcctaa
     5581 tggtattcgc ctgggtattg taaaaccatg gaactctacc tggtttgcga acaccaaaga
     5641 attcgctgac aacctggaca gcgattttaa agtacgtcag tacctgacta aggaactggc
     5701 taaagcgtcc gtatctcgta tcgttatcga gcgtccggct aagagcatcc gtgtaaccat
     5761 tcacactgct cgcccgggta tcgttatcgg taaaaaaggt gaagacgtag aaaaactgcg
     5821 taaggtcgta gcggacatcg ctggcgttcc tgcacagatc aacatcgccg aagttcgtaa
     5881 gcctgaactg gacgcaaaac tggttgctga cagcatcact tctcagctgg aacgtcgcgt
     5941 tatgttccgt cgtgctatga agcgtgctgt acagaacgca atgcgtctgg gcgctaaagg
     6001 tattaaagtt gaagttagcg gccgtctggg cggcgcagaa atcgcacgta ccgaatggta
     6061 ccgcgaaggt cgcgtaccgc tgcacactct gcgtgctgac atcgactaca acacctctga
     6121 agcgcacacc acttacggtg taatcggcgt taaagtgtgg atcttcaaag gcgagatcct
     6181 gggtggtatg gctgctgttg aacaaccgga aaaaccggct gctcagccta aaaagcagca
     6241 gcgtaaaggc cgtaaataag gagcgtcgct gatgttacaa ccaaagcgta caaaattccg
     6301 taaaatgcac aaaggccgta accgcggtct ggcgcagggt acggatgtta gcttcggcag
     6361 cttcggtctg aaagctgttg gccgtggtcg tctgactgcc cgtcagatcg aagcagcacg
     6421 tcgtgctatg acccgtgcag ttaagcgtca aggtaagatc tggatccgtg tgttcccgga
     6481 caaaccgatc actgaaaagc cgctggcagt gcgtatgggt aaaggtaaag gtaacgtgga
     6541 gtattgggtt gccttgattc agccgggtaa agtcctgtat gaaatggacg gtgttccgga
     6601 agagctggcc cgtgaagcat tcaagctggc agcagcgaaa ctgccgatta aaaccacctt
     6661 tgtaactaag acggtgatgt aatgaaagca aaagagctgc gtgagaagag cgttgaagag
     6721 ctgaacaccg agctgctgaa cctgctgcgt gagcagttca acctgcgtat gcaggctgca
     6781 agtggccagc tgcaacagtc tcacctgttg aagcaagtgc gtcgcgatgt cgcacgcgtt
     6841 aagactttac tgaacgagaa ggcgggtgcg taatgaccga taaaatccgt actctgcaag
     6901 gtcgcgttgt tagcgacaaa atggagaaat ccattgttgt tgctatcgaa cgttttgtga
     6961 aacacccgat ctacggtaaa ttcatcaagc gtacgaccaa actgcacgta catgacgaga
     7021 acaacgaatg cggtatcggt gacgtggttg aaatccgcga atgccgtccg ctgtccaaga
     7081 ctaaatcctg gacgctggtt cgcgttgtag agaaagcggt tctgtaatac agtacactct
     7141 ctcgatacga ataaacggct cagaaatgag ccgtttattt tttctaccca tatccttgaa
     7201 gcggtgttat aatgccgcgc cctcgatatg gggattttta acgacctgat tttcgggtct
     7261 cagtagtagt tgacattagc ggagcactaa aatgatccaa gaacagacta tgctgaacgt
     7321 cgccgacaac tccggtgcac gtcgcgtaat gtgtatcaag gttctgggtg gctcgcaccg
     7381 tcgctacgca ggcgtaggcg acatcatcaa gatcaccatc aaagaagcaa ttccgcgtgg
     7441 taaggtcaaa aaaggtgatg tgctgaaggc ggtagtggtg cgcaccaaga agggtgttcg
     7501 tcgcccggac ggttctgtca ttcgcttcga tggtaatgct tgtgttcttc tgaacaacaa
     7561 cagcgagcag cctatcggta cgcgtatttt tgggccggta actcgtgagc ttcgtagtga
     7621 gaagttcatg aaaattatct ctctggcacc agaagtactc taaggagcga atcatggcag
     7681 cgaaaatccg tcgtgatgac gaagttatcg tgttaaccgg taaagataaa ggtaaacgcg
     7741 gtaaagttaa gaatgtcctg tcttccggca aggtcattgt tgaaggtatc aacctggtta
     7801 agaaacatca gaagccggtt ccggccctga accaaccggg tggcatcgtt gaaaaagaag
     7861 ccgctattca ggtttccaac gtagcaatct tcaatgcggc aaccggcaag gctgaccgtg
     7921 taggctttag attcgaagac ggtaaaaaag tccgtttctt caagtctaac agcgaaacta
     7981 tcaagtaatt tggagtagta cgatggcgaa actgcatgat tactacaaag acgaagtagt
     8041 taaaaaactc atgactgagt ttaactacaa ttctgtcatg caagtccctc gggtcgagaa
     8101 gatcaccctg aacatgggtg ttggtgaagc gatcgctgac aaaaaactgc tggataacgc
     8161 agcagcagac ctggcagcaa tctccggtca aaaaccgctg atcaccaaag cacgcaaatc
     8221 tgttgcaggc ttcaaaatcc gtcagggcta tccgatcggc tgtaaagtaa ctctgcgtgg
     8281 cgaacgcatg tgggagttct ttgagcgcct gatcactatt gctgtacctc gtatccgtga
     8341 cttccgtggc ctgtccgcta agtctttcga cggtcgtggt aactacagca tgggtgtccg
     8401 tgagcagatc atcttcccag aaatcgacta cgataaagtc gaccgcgttc gtggtttgga
     8461 tattaccatt accactactg cgaaatctga cgaagaaggc cgcgctctgc tggctgcctt
     8521 tgacttcccg ttccgcaagt aaggtagggt tactaaatgg ctaagcaatc aatgaaagca
     8581 cgcgaagtaa aacgcgtagc tttagctgat aaatacttcg cgaaacgcgc tgaactgaaa
     8641 gcgatcatct ctgatgtgaa cgcttccgac gaagatcgtt ggaacgctgt tctcaagctg
     8701 cagactctgc cgcgtgattc cagcccgtct cgtcagcgta accgctgccg tcaaacaggt
     8761 cgtccgcatg gtttcctgcg gaagttcggg ttgagccgta ttaaggtccg tgaagccgct
     8821 atgcgcggtg aaatcccggg tctgaaaaag gctagctggt aattgtcacc aattgaatca
     8881 cgggagtaaa gacagatgag catgcaagat ccgatcgcgg atatgctgac ccgtatccgt
     8941 aacggtcagg ccgcgaacaa agctgcggtc accatgcctt cctccaagct gaaagtggca
     9001 atcgccaacg tgctgaagga agaaggtttt attgaagatt ttaaagttga aggcgacacc
     9061 aagcctgaac tggaacttac tctgaagtat ttccagggca aagctgttgt agaaagcatt
     9121 cagcgtgtca gccgcccagg tctgcgcatc tataagcgta aagatgagct gccgaaagtt
     9181 atggcgggtc tgggtatcgc agttgtttct acctctaaag gtgttatgac tgatcgtgca
     9241 gcgcgccagg ctggtcttgg tggcgaaatt atctgctacg tagcctaatc ggaggaaaaa
     9301 atgtctcgtg ttgctaaagc accggtcgtt gttcctgccg gcgttgacgt aaaaatcaac
     9361 ggtcaggtta ttacgatcaa aggtaaaaac ggcgagctga ctcgtactct caacgatgct
     9421 gttgaagtta aacatgcaga taataccctg accttcggtc cgcgtgatgg ttacgcagac
     9481 ggttgggcac aggctggtac cgcgcgtgcc ctgctgaact caatggttat cggtgttacc
     9541 gaaggcttca ctaagaagct gcagctggtt ggtgtaggtt accgtgcagc ggttaaaggc
     9601 aatgtgatta acctgtctct gggtttctct catcctgttg accatcagct gcctgcgggt
     9661 atcactgctg aatgtccgac tcagactgaa atcgtgctga aaggcgctga taagcaggtg
     9721 atcggccagg ttgcagcgga tctgcgcgcc taccgtcgtc ctgagcctta caaaggcaag
     9781 ggtgttcgtt acgccgacga agtcgtgcgt accaaagagg ctaagaagaa gtaaggtaac
     9841 actatggata agaaatctgc tcgtatccgt cgtgcgaccc gcgcacgccg caagctccag
     9901 gagctgggcg caactcgcct ggtggtacat cgtaccccgc gtcacattta cgcacaggta
     9961 attgcaccga acggttctga agttctggta gctgcttcta ctgtagaaaa agctatcgct
    10021 gaacaactga agtacaccgg taacaaagac gcggctgcag ctgtgggtaa agctgtcgct
    10081 gaacgcgctc tggaaaaagg catcaaagat gtatcctttg accgttccgg gttccaatat
    10141 catggtcgtg tccaggcact ggcagatgct gcccgtgaag ctggccttca gttctaaggt
    10201 agaggtgtaa gatggctcac atcgaaaaac aagctggcga actgcaggaa aagctgatcg
    10261 cggtaaaccg cgtatctaaa accgttaaag gtggtcgtat tttctccttc acagctctga
    10321 ctgtagttgg cgatggtaac ggtcgcgttg gttttggtta cggtaaagcg cgtgaagttc
    10381 cagcagcgat ccagaaagcg atggaaaaag cccgtcgcaa tatgattaac gtcgcgctga
    10441 ataacggcac tctgcaacac cctgttaaag gtgttcacac gggttctcgc gtattcatgc
    10501 agccggcttc cgaaggtacc ggtatcatcg ccggtggtgc aatgcgtgcc gttctggaag
    10561 tcgctggggt tcataacgtt ctggctaaag cgtatggttc caccaacccg attaacgtgg
    10621 ttcgtgcaac tatcgatggc ctggaaaata tgaattctcc agaaatggtc gctgccaagc
    10681 gtggtaaatc cgttgaagaa attctgggga aataaaccat ggcaaagact attaaaatta
    10741 ctcaaacccg cagtgcaatc ggtcgtctgc cgaaacacaa ggcaacgctg cttggcctgg
    10801 gtctgcgtcg tattggtcac accgtagagc gcgaggatac tcctgctatt cgcggtatga
    10861 tcaacgcggt ttccttcatg gttaaagttg aggagtaaga gatgcgttta aatactctgt
    10921 ctccggccga aggctccaaa aaggcgggta aacgcctggg tcgtggtatc ggttctggcc
    10981 tcggtaaaac cggtggtcgt ggtcacaaag gtcagaaatc tcgttctggc ggtggcgtac
    11041 gtcgcggttt cgagggtggt cagatgcctc tgtaccgtcg tctgccgaaa ttcggcttca
    11101 cttctcgtaa agcagcgatt acagccgaag ttcgtctgtc tgacctggct aaagtagaag
    11161 gcggtgttgt agacctgaac acgctgaaag cggctaacat tatcggtatc cagatcgagt
    11221 tcgcgaaagt gatcctggct ggcgaagtca ctactccggt aactgttcgt ggcctgcgtg
    11281 ttactaaagg cgctcgtgct gctatcgaag ctgctggcgg taaaatcgag gaataagtag
    11341 cagatggcta aacaaccggg attagatttt caaagtgcca aaggtggctt aggcgagctg
    11401 aaacgcagac tgctgtttgt tatcggtgcg ctgattgtgt tccgtattgg ctcttttatt
    11461 ccgatccctg gtattgatgc cgctgtactt gccaaactgc ttgagcaaca gcgaggcacc
    11521 atcattgaaa tgtttaacat gttctctggt ggtgctctca gccgtgcttc tatctttgct
    11581 ctggggatca tgccatatat ttcggcatcg atcattatcc agctgctgac ggtggttcac
    11641 ccaacgttgg cagaaattaa gaaagaaggg gagtctggtc gtcgtaagat cagccagtac
    11701 acccgctacg gtactctggt gctggcaata ttccagtcga tcggtattgc taccggtctg
    11761 ccgaatatgc ctggtatgca aggcctggtc attaacccgg gctttgcatt ctacttcacc
    11821 gctgttgtaa gtctggtcac aggaaccatg ttcctgatgt ggttgggcga acagattact
    11881 gaacgaggta tcggcaacgg tatttcaatc attatcttcg ccggtattgt cgcgggactc
    11941 ccgccagcca ttgcccatac tatcgagcaa gcgcgtcaag gcgacctgca cttcctcgtg
    12001 ttgctgttgg ttgcagtatt agtatttgca gtgacgttct ttgttgtatt tgttgagcgt
    12061 ggtcaacgcc gcattgtggt aaactacgcg aaacgtcagc aaggtcgtcg tgtctatgct
    12121 gcacagagca cacatttacc gctgaaagtg aatatggcgg gggtaatccc ggcaatcttc
    12181 gcttccagta ttattctgtt cccggcgacc atcgcgtcat ggttcggggg cggtactggt
    12241 tggaactggc tgacaacaat ttcgctgtat ttgcagcctg ggcaaccgct ttatgtgtta
    12301 ctctatgcgt ctgcaatcat cttcttctgt ttcttctaca cggcgttggt tttcaacccg
    12361 cgtgaaacag cagataacct gaagaagtcc ggtgcatttg taccaggaat tcgtccggga
    12421 gagcaaacgg cgaagtatat cgataaagta atgacccgcc tgaccctggt tggtgcgctg
    12481 tacattacct ttatctgcct gatcccggag ttcatgcgtg atgcaatgaa agtaccgttc
    12541 tactttggtg ggacctcact gcttatcgtt gttgtcgtga ttatggactt tatggctcaa
    12601 gtgcaaactc tgatgatgtc cagtcagtat gagtctgcat tgaagaaggc gaacctgaaa
    12661 ggctacggcc gataattggt cgcccgagaa gttacggaga gtaaaaatga aagttcgtgc
    12721 ttccgtcaag aaattatgcc gtaactgcaa aatcgttaag cgtgatggtg tcatccgtgt
    12781 gatttgcagt gccgagccga agcataaaca gcgccaaggc tgattttttc gcatattttt
    12841 cttgcaaagt tgggttgagc tggctagatt agccagccaa tcttttgtat gtctgtgcgt
    12901 ttccatttga gtatcctgaa aacgggcttt tcagcatgga acgtacatat taaatagtag
    12961 gagtgcatag tggcccgtat agcaggcatt aacattcctg atcataagca tgccgtaatc
    13021 gcattaactt cgatttatgg cgtcggcaag acccgttcta aagccatcct ggctgcagcg
    13081 ggtatcgctg aagatgttaa gatcagtgag ctgtctgaag gacaaatcga cacgctgcgt
    13141 gacgaagttg ccaaatttgt cgttgaaggt gatctgcgcc gtgaaatcag catgagcatc
    13201 aagcgcctga tggatcttgg ttgctatcgc ggtttgcgtc atcgtcgtgg tctcccggtt
    13261 cgcggtcagc gtaccaagac caacgcacgt acccgtaagg gtccgcgcaa accgatcaag
    13321 aaataatcgg ggtgattgaa taatggcaaa ggcaccaatt cgtgcacgta aacgtgtaag
    13381 aaaacaagtc tctgacggcg tggctcatat ccatgcttct ttcaacaaca ccatcgtgac
    13441 tatcactgat cgtcagggta acgcgttggg ttgggcaaca gccggtggtt ccggtttccg
    13501 tggttctcgc aaatccactc cgtttgcagc tcaggttgca gcagagcgtt gcgctgacgc
    13561 cgtgaaagaa tacggcatca agaatctgga agttatggtt aaaggtccgg gtccaggccg
    13621 cgaatctact attcgtgctc tgaacgccgc aggtttccgc atcactaaca ttactgatgt
    13681 gactccgatc cctcataacg gttgtcgtcc gccgaaaaaa cgtcgcgtat aacgcttcgt
    13741 tttccaggtt tgttggagaa agaaaatggc aagatatttg ggtcctaagc tcaagctgag
    13801 ccgtcgtgag ggcaccgact tattccttaa gtctggcgtt cgcgcgatcg ataccaagtg
    13861 taaaattgaa caagctcctg gccagcacgg tgcgcgtaaa ccgcgtctgt ctgactatgg
    13921 tgtgcagttg cgtgaaaagc aaaaagttcg ccgtatctat ggtgtgctgg agcgtcagtt
    13981 ccgtaactac tacaaagaag cagcacgtct gaaaggcaac accggtgaaa acctgttggc
    14041 tctgctggaa ggtcgtctgg acaacgttgt ataccgtatg ggcttcggtg ccactcgtgc
    14101 agaagcacgt cagctggtta gccataaagc aattatggta aacggtcgtg ttgttaacat
    14161 cgcttcttat caggttagtc cgaatgacgt tgtaagcatt cgtgagaaag cgaagaagca
    14221 gtctcgcgtg aaagccgctc tggagctggc tgagcagcgt gaaaagccaa cctggctgga
    14281 agttgatgct ggcaagatgg aaggtacgtt taagcgtaag ccggagcgtt ctgatctgtc
    14341 tgcggacatt aacgaacacc tgatcgtcga gctttactcc aagtaaagct tagtaccaaa
    14401 gagaggacac aatgcagggt tctgtgacag agtttctaaa accgcgcctg gttgatatcg
    14461 agcaagtgag ttcgacgcac gccaaggtga cccttgagcc tttagagcgt ggctttggcc
    14521 atactctggg taacgcactg cgccgtattc tgctctcatc gatgccgggt tgcgcggtga
    14581 ccgaggttga gattgatggt gtactacatg agtacagcac caaagaaggc gttcaggaag
    14641 atatcctgga aatcctgctc aacctgaaag ggctggcggt gagagttcag ggcaaagatg
    14701 aagttattct taccttgaat aaatctggca ttggccctgt gactgcagcc gatatcaccc
    14761 acgacggtga tgtcgaaatc gtcaagccgc agcacgtgat ctgccacctg accgatgaga
    14821 acgcgtctat tagcatgcgt atcaaagttc agcgcggtcg tggttatgtg ccggcttcta
    14881 cccgaattca ttcggaagaa gatgagcgcc caatcggccg tctgctggtc gacgcatgct
    14941 acagccctgt ggagcgtatt gcctacaatg ttgaagcagc gcgtgtagaa cagcgtaccg
    15001 acctggacaa gctggtcatc gaaatggaaa ccaacggcac aatcgatcct gaagaggcga
    15061 ttcgtcgtgc ggcaaccatt ctggctgaac aactggaagc tttcgttgac ttacgtgatg
    15121 tacgtcagcc tgaagtgaaa gaagagaaac cagagttcga tccgatcctg ctgcgccctg
    15181 ttgacgatct ggaattgact gtccgctctg ctaactgcct taaagcagaa gctatccact
    15241 atatcggtga tctggtacag cgtaccgagg ttgagctcct taaaacgcct aaccttggta
    15301 aaaaatctct tactgagatt aaagacgtgc tggcttcccg tggactgtct ctgggcatgc
    15361 gcctggaaaa ctggccaccg gcaagcatcg ctgacgagta accggatcac aggttaaggt
    15421 tttactgaga aggataaggt catgcgccat cgtaagagtg gtcgtcaact gaaccgcaac
    15481 agcagccatc gccaggctat gttccgcaat atggcaggtt cactggttcg tcatgaaatc
    15541 atcaagacga ctctgcctaa agcgaaagag ctgcgccgcg tagttgagcc gctgattact
    15601 cttgccaaga ctgatagcgt tgctaatcgt cgtctggcat tcgcccgtac tcgtgataac
    15661 gagatcgtgg caaaactgtt taacgaactg ggcccgcgtt tcgcgagccg tgccggtggt
    15721 tacactcgta ttctgaagtg tggcttccgt gcaggcgaca acgcgccgat ggcttacatc
    15781 gagctggttg atcgttcaga gaaagcagaa gctgctgcag agtaatctga agcaacgtaa
    15841 aaaaacccgc cccggcgggt ttttttatac ccgtagcatc cccacttatc tacaatagct
    15901 gtactctttt tgttcatccc ctggagtata tatgtggtta cttgaccagt gggcagagcg
    15961 tcatatagca gaagcgcaag cgaaaggtga gtttgataac ctggcaggta gcggcgaacc
    16021 attgatactg gctgatgatt ctcatgtgcc accggaatta cgtgcggggt atcgcttgct
    16081 gaagaatgcc ggttgcttac cgccagaact tgagcagcgg agggaagcaa ttcagcttct
    16141 ggatatcctg agaggtatcc gtcacgatga tccgcaatat caagaggtta gccgtcgatt
    16201 gtcattactg gaattgaagc tgcgacaagc tggactgagc accgattttt tacgcggcga
    16261 ttatgctgac aagttgttga ataaaatcaa cgataactag tggagtatgt atgtatcgca
    16321 ttggtgagct ggcaaaaatg gcggaagtaa cacccgacac gattcgttat tacgaaaaac
    16381 agcagatgat ggagcatgaa gtgcgtactg aaggtgggtt tcgcctgtat accgaaagcg
    16441 atctccagcg attgaaattt atccgccatg ccagacaact aggtttcagt ctagagtcga
    16501 tccgcgagtt gctgtcgatc cgtatcgatc ctgaacacca tacctgtcag gagtcaaaag
    16561 ccattgtgca ggaaagattg caggaagtcg aagcacggat agccgagttg cagagtatgc
    16621 agcgttccct ccaacgcctt aacgatgcct gttgtgggac cgcccatagc agtgtttatt
    16681 gctcgattct tgaagctctt gaacaagggg cgagtggcgt taagagtggc tgttgatttt
    16741 ttacactggc aggattacac tcgcgccgtt aaataaccaa ttggagtttt tatgagccga
    16801 tatcagcata ctaaagggca gataaaggat aatgcgatag aagcattact acatgatccc
    16861 ttattccgac agcgcgtaga gagaaataag aaagggaaag gcagttacat gcgaaaagga
    16921 aaacatggca atcgggggaa ctgggaggcc agtggcaaga aagtaaatca cttttttacc
    16981 actggccttc tgctttcagg cgcttgttaa gagcggttat tctgctcttt cagcaaatca
    17041 cgaatttctg tcagtaatac ttcttcttta gttggtgtag gcgcggctgc tggttcttct
    17101 tttttccgat tcagtttgtt gattagctta atcgccataa agatggcaaa ggctacaatc
    17161 agaaaatcaa agacgttttg aatgaagaca ccgtaatgca tcacaacagc agggatatcc
    17221 ccctgcgcat cgcgtagcgt gacagcaaac tgtttgaaat cgatcccgcc aattaataac
    17281 cccagaggtg gcataatgat atcggcaacc agtgaagata caatcttccc gaatgccgca
    17341 ccgataatga cacccaccgc caaatccacc acgttcccgc gcatcgcaaa ttcgcgaaat
    17401 tctttaataa tgctcatgtt attctcccta tgaagccgac aatcataagt ctaacaaatg
    17461 ttaagctatt ttcctgccag gagtgaattt aattaatccc tgataatcaa agggaaataa
    17521 taaggcgtca ttagacgcct tattaattac aagaagaaag gacttggctg gaagagtctt
    17581 tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc gccttgctca
    17641 atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc tccaataatc
    17701 gttcctggcg gtagcttgat ttcatcaatg actctgccga caacgcgtga ggtgctttca
    17761 tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcagtga ggaaacacca
    17821 acaatatcag cttttcgcac atggctaagc aacgcagaaa tagttgcttg ttgcggtgaa
    17881 atcgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg ctggatcaat
    17941 accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat attggcttcg
    18001 tcatcgttgg tgacagcaat aaacagatca acttgatcga tatgttcttc ggccagcagt
    18061 tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc cgccagttcg
    18121 gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc cagacgacgc
    18181 gccagccctg caccgatatt accgccgcca accagcatga tccgcttata cggtttttcc
    18241 agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat aaagaacact
    18301 tcatcaccag cttcaacaat ggtcgaacct tgcggacgaa tggggcgatc gtggcggaaa
    18361 attgctgcca cacgagtatc gatatggggc atatgttcgc gcatggtcga aagtgcatta
    18421 ccaatcagcg ggccgccata ataggcttta accacagcca ggctgacttt accctcagcg
    18481 aagttcacca cctgcaatgc gccgggatac tcaatcagtc ggtaaatatt atcgataacc
    18541 aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa tagtttatcg
    18601 gcatcgcgca cgtagtctgg tgagcggata cgagcgatgc gattaggggt gttgaaaagt
    18661 gagtaggcta cctggcaggc aaccatattg gtttcatctg aactagttac agcaaccagc
    18721 atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc atgcccctgc
    18781 acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt ggtatcgaca
    18841 acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc aacctggccg
    18901 gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt tttgattagc
    18961 ttagcgtaaa agaagccgtc gccctcttcg gcaccaggca gattttgctt acccggttgt
    19021 tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa ggctttaatc
    19081 tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt tccaccggat
    19141 tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag ttccgggata
    19201 tcgcgatcgc gacgtaacca tttaatatcc ggatggcgac gaatcacacc ggttgctgaa
    19261 caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg ggaagggtag
    19321 cggccatcac cttgtttcac ggtcgccttc ataccaaggc gttttaaatt gtcgtaaacg
    19381 cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg tgccacctca
    19441 aggatatgcg ttgttttacc gccgggggca gcgcaaagat ccagaatgtg ttcaccgttt
    19501 tgtggcgcaa gccaggccat gcaaccttgt gctgatgcat cctgaacggt aacccatcct
    19561 tcttcaaaac ctggtagcac atgaacaggt gcaggtgttt ccagccgtac agcatcaggg
    19621 taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag ccagctgtcg
    19681 cgggaatgat gcgtacgatt aacgcgcagc cacattggcg gacgctggtt attggcttca
    19741 acgatggatt gccattgctc tggatacgct ttttgcagac gcttcagcaa ccaggaagga
    19801 tgcagataac atgcatcact ggcattaaac tcggctaata actcttcttg ctgacgctgg
    19861 aactggcgta atacaccgtt tatcaaccct ttaagttgcg ggcgcttaat tgcgatagcg
    19921 ccttcaaccg tttcagccag cgcagcatga ggtggtatgc gggtataaag cagttgatac
    19981 aaaccaacca taatcaggta atgcacggtc cgctgtttgc cggtcatcgg acgggccatt
    20041 aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa gcacaactct
    20101 tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag aatgttgctt
    20161 aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct acgtaaatta
    20221 cgttgttttt tcataagtat aaaaatgccc ggcaagaccg ggcttagaag agtggactat
    20281 cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag gtcttgcgcg
    20341 ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc atcaccagtc
    20401 gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc gttggttgcc
    20461 gtatcaatga ccgatgcttt ccagacttta accggctgtc cttcaatttc cagccagctc
    20521 attggccatg gattgaaagc gcgaatgcag cgttcaagct gtgctgccga aagtgaccag
    20581 tcaatacgcg cttcttcttt actcaacttt tcggcgtaag tgacaagagt ttcgtcctga
    20641 acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag cccttgtggg
    20701 ccaagctctg ccagcttgtc gtacagcgta ccactggtat cttctgcagt aatcgggcag
    20761 gagagcttat agagcatgtc tccggtgtct aaaccaacat ccatttgcat aatggtcaca
    20821 ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc gcgccagcgt
    20881 ggcagcagtg aaccatgaac gttgatacag ccaagacgcg gcatctccag cactgcttta
    20941 ggcagaatta agccataggc gacgacaacc ataacatcag cctgtagatc ggcgaccagt
    21001 tgctggtttt cttgtggacg cagggaaacc ggttgaaaaa cgggcagacc tttttcctca
    21061 gccagaactt taaccgggct gggcatcagt tttttaccgc gtcctgccgg tcggtctggc
    21121 tgggtgaaca cgccaacgac gttatgatca gaagacaaca gcgcgtcgag atgacgcgct
    21181 gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt agttcttatc
    21241 cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaata cgttgttgtt
    21301 tcagcggtga cagataatcc ataaacagtt tgccgaccag gtgatccatc tcatgctgaa
    21361 tacagatggc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg cggtccaggg
    21421 cgcgaatttt aactttctct gcgcgtggca ctaaagcacg ttgttcaggg atcgacaggc
    21481 atccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg attaacacca
    21541 gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg atatcgacct
    21601 gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata tcatcgacga
    21661 tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga agccgctcgt
    21721 ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt caggagttag
    21781 aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc actgaccaat
    21841 cgcaaagatt gctaaggctg cttatggcag ggagataagg atggtcgata cagaaatttg
    21901 gctgcgttta atgagcatca gcagcttgta cggcgatgat atggtccgta tagctcactg
    21961 gctggcaaaa cagtcgcata ttgatgcggt tgtattgcag caaactgggc ttacattgcg
    22021 gcaggcacaa cgctttcttt catttccgcg aaagagtatc gaaagctcac tttcttggtt
    22081 ggagcaaccc aaccatcatt taattcctgc ggacagcgaa ttttatcctc ctcaacttct
    22141 tgcgacgaca gattaccccg gcgcactgtt tgttgaagga gaactgcacg cgctgcattc
    22201 atttcagctt gccgtagtgg ggagtcgggc gcattcatgg tatggcgagc gatggggacg
    22261 attattttgc gaaactctgg cgacgcgtgg agtgacaatt acgagtggac tggcgcgtgg
    22321 aatcgatggt gtggcgcata aagcggcctt acaggtaaat ggcgtcagca ttgctgtatt
    22381 ggggaatgga cttaatacca ttcatccgcg tcgccatgcc cgactggcta ccagtttgct
    22441 tgaacatggt ggtgctcttg tctcggaatt tcccctcgat gttcctcctc ttgcttacaa
    22501 tttcccacga agaaatcgca ttatcagtgg tctaagtaaa ggtgtactgg tggtggaagc
    22561 ggctttgcgc agtggttcgc tggtgacagc acgttgtgcg cttgagcagg ggcgtgaagt
    22621 ttttgccttg ccgggtccat tagggaatcc gggaagcgaa gggcctcact ggttaataaa
    22681 acaaggtgcg attcttgtga cggaaccgga agaaattctg gaaaacttgc aatttggatt
    22741 gcactggttg tcagacgccc ctgaaaattc attttattca ccagatcagg aagacgtggc
    22801 attgccattt cctgagctcc tggctaacgt aggagatgag gtaacacctg ttgacgtcgt
    22861 cgctgaacgt gccggccaac ctgtgccaga ggtagtaact caactactcg aactggagtt
    22921 agcaggatgg atcgcagctg tacccggcgg ctatgtccga ttgaggaggg catgccatgt
    22981 tcgacgtact aatgtatttg tttgaaacct atattcacac agaagctgag ttgcgtgtgg
    23041 atcaagacaa acttgaacag gatcttaccg acgcaggatt tgatcgagaa gatatctaca
    23101 atgccctgct ttggctggaa aagcttgcgg attatcagga aggactggca gaaccgatgc
    23161 aactggcctc tgatccactc tccatgcgta tttatacccc tgaagagtgt gaaaggctgg
    23221 atgccagctg tcgtggtttt ctgcttttcc ttgagcaaat tcaggtgctt aaccttgaaa
    23281 cccgtgaaat ggtgatagag cgagtgctcg cgctggatac cgcagagttc gacctggaag
    23341 acctgaaatg ggtaatcctg atggtgttgt tcaatattcc gggctgcgaa aatgcgtacc
    23401 agcaaatgga agaattactc tttgaagtga atgaaggtat gctgcattaa tcatttcttt
    23461 aattcagcat aagttgttat ggcgaaatca gcactgttca cggtgcgtaa taatgagtcc
    23521 tgcccaaagt gcggggctga actggttatt cgatccggga aacacggtcc gtttcttgga
    23581 tgctcacagt atccggcgtg tgactacgtc cgtcctctga aatcttcagc ggatggacat
    23641 attgtcaaag ttctggaggg gcaggtttgc cctgcatgtg gcgcaaatct ggtattacgc
    23701 cagggacgct ttggtatgtt tattggttgc agtaactacc ctgaatgcga acataccgaa
    23761 cttatcgata aaccggacga aacagcaatt acatgccccc aatgccggac gggccatctg
    23821 gtccagcgcc gctcccgtta tggcaaaacg tttcactcct gtgatcgcta cccggagtgt
    23881 caatttgcca ttaacttcaa acccatagct ggagaatgtc ctgagtgtca ttatccgcta
    23941 ctcatcgaaa agaaaaccgc gcagggtgta aaacactttt gtgccagtaa acaatgtgga
    24001 aagccggttt cggcggaata ataacgtgaa taataacctg caaggagacg ctatcgcagc
    24061 tgcgatagat gtcctcaatg aagaacgtgt catcgcctat ccaacggaag ccgttttcgg
    24121 tgtcgggtgc gatcctgata gcgaaacagc agtgatgcga ctgttggagt taaaacagcg
    24181 tccggttgat aaggggctga ttctaatcgc cgcaaattac gagcagctta aaccctatat
    24241 tgatgacacc atgctgactg acgcgcagcg tgaaactata ttttctcgtt ggccaggtcc
    24301 tgtcaccttt gtttttcccg cgcctgcgac aacaccgcgc tggttgacgg gccgctttga
    24361 ttcgcttgct gtacgagtca ccgaccatcc gttggtggtt gctttgtgcc aggcttatgg
    24421 taaaccgctg gtttctacca gtgccaactt gagtggattg ccaccttgtc gaacagtaga
    24481 cgaagttcgc gcacaatttg gcgcggcgtt cccggttgtg cctggtgaaa cgggggggcg
    24541 tttaaatcct tcagaaatcc gcgatgccct gacgggtgaa ctgtttcgac aggggtaaca
    24601 taatggaaac ctatgctgtt tttggtaatc cgatagccca cagcaaatcg ccattcattc
    24661 atcagcaatt tgctcagcaa ctgaatattg aacatcccta tgggcgcgtg ttggcaccca
    24721 tcaatgattt catcaacaca ctgaacgctt tctttagtgc tggtggtaaa ggtgcgaatg
    24781 tgacggtacc ttttaaagaa gaggcttttg ccagagcgga tgagcttact gaacgggcag
    24841 cgttggctgg tgctgttaat accctcaagc ggttagaaga tggacgcctg ctgggtgaca
    24901 ataccgatgg tataggtttg ttaagcgatc tggaacgtct gtcttttatc cgtcctggtt
    24961 tacgtattct gctcattggc gctggtggag catctcgcgg cgtactactg ccactccttt
    25021 ccctggactg tgcggtgaca atcactaatc ggacggtatc ccgcgcggaa gagttggcta
    25081 aattgtttgc gcacactggc agtattcagg cgttgggtat ggacgaactg gaaggccatg
    25141 agtttgatct cattattaat gcaacatcca gtggcatcag tggtgatatt ccggcgatcc
    25201 cgtcatcgct cattcatcca ggcatttatt gctatgacat gttctatcag aaaggaaaaa
    25261 ctcctttttt ggcatggtgt gagcagcgag gctcaaagcg taatgctgat ggtttaggaa
    25321 tgctggtggc acaggcggct catgccttcc ttctttggca tggcgttctg cctgatgtag
    25381 aaccggtcat caaactattg caacaggaat tgtcagcgtg aatcaggcca tccagtttcc
    25441 ggatagggaa gagtgggacg agaataaaca atgtgtatgt tttcccgctc tcgtgaatgg
    25501 tatgaaactg acatgtgcga tctctggcga gagtctggcg tatcgcttta ctggagatac
    25561 gccagaacag tggttagcga gttttcgtca gcatcgctgg gacctggaag aagaagcgga
    25621 aaacttaatt caggaacaaa gtgaagatga tcaaggttgg gtctggttac cttgatccag
    25681 atattcgtcc ttccatttca cgtagttatt tgcggaatag cgtaacccag ccttctcttc
    25741 atcacttaac gggcggatct gtttgacggg gctaccgaga tacagatatc cgctctccag
    25801 ccatttattt tgtgggacca ggctacccgc accaatcatc acatcatctt ctactattgc
    25861 gccatcaagt aaaattgagc ccatcccaac caaaactcga ttgccaatgg tgcagccgtg
    25921 gagcatcacc ttgtgaccaa cagtgacatc ttcgccaatg gttaatgggt tgccatctgg
    25981 gttgtacgag gatttatgag tgacatgcaa catactgcca tcctggatat tggtgcgtgc
    26041 tccgatctgt acataatgta catctccacg aatcacaacg agcggccaga tccccacatc
    26101 atcagccaga cgaacgtcac caatcacgac gctgctatcg tcgatcatta cgcgctgacc
    26161 gatttgtgga aaaagatcgc ggtatgggcg taaaacatca gacatactca cctcaacaat
    26221 aaatgattta ctaatgactt tgggtgcatt attggccttg tgcaagtctt ttagcatgca
    26281 aaaaagcacc gttttgtgtg cgaatgcagc aaaaagggtg aaaaagcaac aaacagaaaa
    26341 aaagatcaaa aaagtacttg tgcaaaaaat tgggatccct ataatgcgcc tccgttgaga
    26401 cgacaacgtg aaacacttca caagatggtc gtaacaacaa agagaaaaaa tcctgaaatt
    26461 cggggttgac tctgaaagag gaaagcgta
//
LOCUS       DAPHCV010000072        26373 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.072, whole genome shotgun sequence.
ACCESSION   DAPHCV010000072 DAPHCV010000000
VERSION     DAPHCV010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.072"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(113..2686)
                     /gene="clpB"
                     /locus_tag="RH033_002949"
     CDS             complement(113..2686)
                     /gene="clpB"
                     /locus_tag="RH033_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDV2992103.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMIRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2816..3547)
                     /gene="pgeF"
                     /locus_tag="RH033_002950"
     CDS             complement(2816..3547)
                     /gene="pgeF"
                     /locus_tag="RH033_002950"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HDV2992104.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEIAAAHAGWRGLCSGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASTAFIQHGDKYLADIYQLARQRLASVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3544..4524)
                     /gene="rluD"
                     /locus_tag="RH033_002951"
     CDS             complement(3544..4524)
                     /gene="rluD"
                     /locus_tag="RH033_002951"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HDV2992105.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPEDMVELIEVMRADFEEHKDEVDWL"
     gene            4659..5396
                     /gene="bamD"
                     /locus_tag="RH033_002952"
     CDS             4659..5396
                     /gene="bamD"
                     /locus_tag="RH033_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HDV2992106.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQLNAQAEKVAKIIAANSSNT"
     gene            5500..6847
                     /locus_tag="RH033_002953"
                     /pseudo
     CDS             5500..6847
                     /locus_tag="RH033_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
     gene            7108..7449
                     /gene="raiA"
                     /locus_tag="RH033_002954"
     CDS             7108..7449
                     /gene="raiA"
                     /locus_tag="RH033_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDV2992107.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    7552..7677
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            7553..7600
                     /gene="pheL"
                     /locus_tag="RH033_002955"
     CDS             7553..7600
                     /gene="pheL"
                     /locus_tag="RH033_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HDV2992108.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            7699..8859
                     /gene="pheA"
                     /locus_tag="RH033_002956"
     CDS             7699..8859
                     /gene="pheA"
                     /locus_tag="RH033_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HDV2992109.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPQWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGDITRSMKVLGCYPSENVVPVDPT"
     gene            complement(8902..10023)
                     /gene="tyrA"
                     /locus_tag="RH033_002957"
     CDS             complement(8902..10023)
                     /gene="tyrA"
                     /locus_tag="RH033_002957"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HDV2992110.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAAEIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRH"
     gene            complement(10034..11104)
                     /gene="aroF"
                     /locus_tag="RH033_002958"
     CDS             complement(10034..11104)
                     /gene="aroF"
                     /locus_tag="RH033_002958"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HDV2992111.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            11314..11679
                     /locus_tag="RH033_002959"
     CDS             11314..11679
                     /locus_tag="RH033_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HDV2992112.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDGGMEDAISG
                     STIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENTSQ
                     LHEVWQKGADENASTIRLN"
     gene            11829..12347
                     /locus_tag="RH033_002960"
     CDS             11829..12347
                     /locus_tag="RH033_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HDV2992113.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            12337..13563
                     /gene="dgcN"
                     /locus_tag="RH033_002961"
     CDS             12337..13563
                     /gene="dgcN"
                     /locus_tag="RH033_002961"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HDV2992114.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMSSSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            13579..14061
                     /locus_tag="RH033_002962"
     CDS             13579..14061
                     /locus_tag="RH033_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDV2992115.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(14138..14485)
                     /gene="rplS"
                     /locus_tag="RH033_002963"
     CDS             complement(14138..14485)
                     /gene="rplS"
                     /locus_tag="RH033_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDV2992116.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(14527..15294)
                     /gene="trmD"
                     /locus_tag="RH033_002964"
     CDS             complement(14527..15294)
                     /gene="trmD"
                     /locus_tag="RH033_002964"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDV2992117.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(15325..15873)
                     /gene="rimM"
                     /locus_tag="RH033_002965"
     CDS             complement(15325..15873)
                     /gene="rimM"
                     /locus_tag="RH033_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDV2992118.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(15892..16140)
                     /gene="rpsP"
                     /locus_tag="RH033_002966"
     CDS             complement(15892..16140)
                     /gene="rpsP"
                     /locus_tag="RH033_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDV2992119.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(16389..17750)
                     /gene="ffh"
                     /locus_tag="RH033_002967"
     CDS             complement(16389..17750)
                     /gene="ffh"
                     /locus_tag="RH033_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDV2992120.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            17917..18708
                     /locus_tag="RH033_002968"
     CDS             17917..18708
                     /locus_tag="RH033_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HDV2992121.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIFQQLIS"
     gene            18729..20015
                     /locus_tag="RH033_002969"
     CDS             18729..20015
                     /locus_tag="RH033_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDV2992122.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(20070..20663)
                     /gene="grpE"
                     /locus_tag="RH033_002970"
     CDS             complement(20070..20663)
                     /gene="grpE"
                     /locus_tag="RH033_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDV2992123.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPDASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            20786..21664
                     /gene="nadK"
                     /locus_tag="RH033_002971"
     CDS             20786..21664
                     /gene="nadK"
                     /locus_tag="RH033_002971"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HDV2992124.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            21750..23411
                     /gene="recN"
                     /locus_tag="RH033_002972"
     CDS             21750..23411
                     /gene="recN"
                     /locus_tag="RH033_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDV2992125.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALAVMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELALLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            23560..23901
                     /gene="bamE"
                     /locus_tag="RH033_002973"
     CDS             23560..23901
                     /gene="bamE"
                     /locus_tag="RH033_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HDV2992126.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(23963..24253)
                     /locus_tag="RH033_002974"
     CDS             complement(23963..24253)
                     /locus_tag="RH033_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HDV2992127.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(24243..24680)
                     /gene="ratA"
                     /locus_tag="RH033_002975"
     CDS             complement(24243..24680)
                     /gene="ratA"
                     /locus_tag="RH033_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HDV2992128.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            24851..25333
                     /gene="smpB"
                     /locus_tag="RH033_002976"
     CDS             24851..25333
                     /gene="smpB"
                     /locus_tag="RH033_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDV2992129.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            25548..25910
                     /gene="ssrA"
                     /locus_tag="RH033_002977"
     tmRNA           25548..25910
                     /gene="ssrA"
                     /locus_tag="RH033_002977"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         6716 a         6951 c         6264 g         6442 t
ORIGIN      
        1 aaaaaatata aaaactttat cgttcgctca tttttcaggc aaaacatctt aaacatagcc
       61 ttttccgtct aatttataga caaaaacgag ccccgaaggg ctcgttttat cattactgga
      121 cggcgacaat ccggtcttca ttaacttcca ggcgaataac tttacccgga accaattcac
      181 cagacagtat ttgctgtgcc agcgggtttt cgatctgctg ctggatagca cgtttcagcg
      241 gacgtgcacc atagaccgga tcgtaaccgt tctcgctcag cagtttcagc gcctcgtcag
      301 aaatgtggat ttcataacca cgttcttcca gacgtttgta cagacgtttc aactgaatct
      361 gcgcaatcga cgcaatgtgc tgttcaccca gcggatggaa gaccaccact tcatcgatac
      421 ggttaatgaa ttccggacgg aagttatggc ttaccacacc gagcaccagc tctttcatgt
      481 gcgcataatc cagttcaccg aagcgttcct gaatcaaatc ggaaccgagg ttagaggtca
      541 taatgacaac cgtattacgg aagtcgaccg ttctcccctg cccgtcagtc agacgcccat
      601 catccagtac ctgcaacaga atgttgaaga catccggatg cgctttttcc acttcatcca
      661 gcaggatgac ggaatacgga cgacgacgca cagcttcggt caggtagcca ccttcttcat
      721 aaccgacata tcccggaggc gcaccaacca gacgagacac agagtgtttc tccataaact
      781 cggacatatc gatacggatc atcgcctcgt cgctatcaaa cataaagttc gccagcgcct
      841 tacaaagctc agttttcccc acaccggttg ggccgaggaa caggaatgaa ccaatcgggc
      901 gatttggatc cgccagcccc gcacggctac gacgaatagc gttagatacc gcatcaaccg
      961 cttcgttctg accaataacg cgatggtgca gttcttgctc catacgcagc agtttttcgc
     1021 gctcgctttc catcatgcga gaaaccggaa tccccgtcca acgcgccagc acttcagcaa
     1081 tttcggcatc ggtcacttta ttacgcaaca gacgcatagt tttgccttcg agctgcgttg
     1141 cggcttccag ttgcttttcc agttccggga ttttgccgta ttgcagttca gacatccgcg
     1201 ccaggtcccc cacacggcga gcctgttcaa tagcgatttt cgcttgttcc agttccgctt
     1261 taatggtctg cgtaccagaa agcgatgctt tctctgcttt ccactcttct tctaactcgg
     1321 agtactgacg ttctttgtcg ctcagttctt cgttgagcat atccaggcgt tttttactgg
     1381 cttcatcaga ctctttcatt aacgcctgtt gttccagttt aagctggatg atacggcgat
     1441 cgagtcggtc gagttcttct ggttttgagt caatctgcat acgaatgctg gatgctgctt
     1501 catcgatcag gtcgatggct ttatccggca gctgacggtc agcaatgtag cgatgagaca
     1561 acgtcgccgc tgcaacaatt gccgggtcag taatttgcac atggtggtgc aattcgtaac
     1621 gttctttcag gccacgcaga atcgcaatgg tatcttcaac agaaggctcg gcaacaaaca
     1681 ctttctggaa acgacgttcc agcgcagcat ctttttcgat gtactggcga tattcgtcaa
     1741 gcgtcgtggc acctacgcag tgcaattcac cacgcgccag cgccggtttc agcatgtttc
     1801 cggcgtccat tgcgccatcg gctttacccg cgccgaccat ggtatgtaat tcgtcgataa
     1861 acaggatgac gttgccttcc tgtttagcaa gatcgttaag tacgcctttt aaacgttctt
     1921 caaactcacc gcgatatttc gccccagcca ccagcgcgcc catatccagc gccagtacgc
     1981 ggcggccttt caacccttcc ggcacttcgc cgttgatgat acgctgcgcc agaccttcaa
     2041 cgatggcagt tttaccgacg cctggttcac caatcagtac cgggttattt ttagtacgac
     2101 gttgcagcac ctgaatggta cggcgaattt cttcatcacg accaatcacc ggatcgagtt
     2161 tgccctgttc ggctcgttct gtaaggtcga tggtatattt tttcaaagcc tgacgttggt
     2221 cttcagcacc ttgatcgttc acgctttcac ctccacgcat ttgttcaatc gcttgagtaa
     2281 tgttggcggt ggtcgcccct gctgctttca ggatgtcggc cagcgtgccg cgagactcaa
     2341 gtgccgccag aacgaacagt tctgacgaga taaagttatc accacgtttt tgcgccagct
     2401 tgtcgcaaag attaagaacg cgcaccagat cctgtgatgg ctggacatca ccaccagtac
     2461 cttcaacctg cggtaaacga tttaatgcct gattgatatc tgtgcgcaac tggccagcat
     2521 taatgccagc ggatgttaat aaaggactaa ccgaaccccc ttcctgattc agcagggcgc
     2581 tcattaaatg aagtggttcg atgaattggt tgtcgtgccc gagtgcaagt gattgggcat
     2641 cggcaagagc aagctggaat ttattagtaa gacgatccag acgcataact cctcccataa
     2701 cggatcaaag ttgctactgg agattaaatg aggtcatccc tcaattattc aaggttattg
     2761 accagattaa tgtgaaaaga aaatcacgcg taccggatcg tcttgattct ttaggttata
     2821 tcagccaaat gaaacttgcc atacgaccgg tggtcttgtc gcgacgataa gagaagaaag
     2881 tctcattttc cgtatatgta caacggtcgc cgccgaaaat ttgttcaaca ccaacgctcg
     2941 ccagacgctg ccgggcaagc tgataaatat ctgccagata cttatcacca tgctgaatga
     3001 aagctgtact cgctttggcg tctactgcca taaacgcctc gcgaacctcc gcccccactt
     3061 caaacgcgcg tggaccaatt gccggcccta accaggcgag aatattttcc ggattatcag
     3121 caaaacagga aaccgtctct tccagcacgc ctgagcacag tccacgccag ccagcatgag
     3181 cggcggcgat ttccgttccc gctcgattgc aaaacagcac ggggaggcag tcggcagtca
     3241 tcactgcgca aaccgtgccc ggcgtgttgc tataagaggc atctgctcgt tttgaggcat
     3301 aaggttcgcc agtaagctta agcacatctt tgccgtgtac ctgctcaagc cagaccggtt
     3361 ttgaaggcaa attgcccgca gcaaaaagtc gcttgcgatt ctcttcaacg tgatccgggt
     3421 tatcgccaca atgagcaccg aggttgagcg aatcatacgg gggcaagctc acgccgccga
     3481 tacgagtgga gctacaggcc gcaacacctt ttggcagcgg ccactgcggg acaatcagct
     3541 tactcataac cagtccactt catccttatg ctcttcgaaa tcggcgcgca tcacctcaat
     3601 cagctccacc atatcttctg gaataggcgc atgccattcc atttcgatac cggagatcgg
     3661 gtgataaaga cgcagcatgg ttgcatgaag cgcctggcgg tcaaacttac gcagcgtgga
     3721 gataaacgct tccgaagcac cttttggcgg acgcggacgg ccaccataaa ccggatcgcc
     3781 caccagcgga tgagtgatat gggccatatg cacgcggatc tggtgcgtac gtccggtttc
     3841 cagacgcaac cgcagacgcg tgtgcacacg gaagtgttcc atgatgcgat agtgagtgac
     3901 cgccggttta cccatcggat gcaccgccat gtgagtacgt ttggtcgggt ggcggctgat
     3961 tggttcatct actgtaccgc cagcggtcat atgaccaatt gccaccgctt catactcacg
     4021 agtaatttca cgccgttgca aagattcgac taaacgcgtc tgagccggaa cggtttttgc
     4081 cacaaccatc aggccggtgg tgtctttatc cagacgatgg acgatgcccg cacgcggtac
     4141 atcggcaatg ggcggataat aatgaagcaa cgcattcagt accgtaccat ccgggttacc
     4201 cgcgccagga tgtaccacca ggtcgcgtgg tttattaatg acgatgatat cttcatcttc
     4261 atagacgata tccagcggga tatcctgcgg ttcaaaacgc gcttcttctt caatctcagc
     4321 gttgatggcc acctgctcgc cacccaatac tttttctttc ggcttatcac aaactttgcc
     4381 gttaaccagc acgcgctggt cgaggatcca ttcttttatt cgcgaacgtg aataatccgg
     4441 gaacatttcg gccaaagcct gatctaagcg ttgaccgagt tggttttcgg acaccgttgc
     4501 agtgagctgt actcgttgtg ccatatatac tgcttcttcg tttatcgttg gggttttacg
     4561 gctttgccgt ttaatatagt gtgctattgt agctggtctt aaccgggagc aggaacagag
     4621 aatctcccgt attacatttt gaggaaagtc aaaacgtcat gacgcgcatg aaatatctgg
     4681 tggcagccgc cacactaagc ctgtttttgg cgggttgctc ggggtcaaag gaagaagtac
     4741 ctgataatcc gccaaatgaa atttacgcga ctgcacaaca aaagctgcag gacggtaact
     4801 ggagacaggc aataacgcaa ctggaagcgt tagataatcg ctatccgttt ggtccgtatt
     4861 cgcagcaggt gcagctggat ctcatctacg cctactataa aaacgccgat ttgccgttag
     4921 cacaggctgc catcgatcgt tttattcgcc ttaacccgac ccatccgaat atcgattatg
     4981 tcatgtacat gcgtggcctg accaatatgg cgctggatga cagtgcgctg caagggttct
     5041 ttggcgttga ccgtagcgat cgcgatcctc aacatgcacg agctgcgttt agtgactttt
     5101 ccaaactggt gcgcggctat ccgaacagtc agtacaccac cgatgccacc aaacgtctgg
     5161 tattcctgaa agatcgtctg gcgaaatatg aatactccgt tgccgagtac tatacagaac
     5221 gtggtgcatg ggttgccgtc gttaaccgcg tagaaggcat gttgcgcgac tacccggata
     5281 cccaggctac gcgtgatgcg ctgccgctga tggaaaatgc ataccgtcag atgcagttga
     5341 atgcgcaagc tgaaaaagta gcgaaaatca tcgccgcaaa cagcagcaat acataacaga
     5401 aacctgaaac acaaaacggc agcccttgag ctgtaatgcc gatcagttaa ggatcagttg
     5461 accgatccag tggctgtgta agaatccgga aacgctcact tgtttccgga tttttttatg
     5521 cacattggac aggctcttga tctggtatcc cgttacgatt ctctgcgtaa cccactgact
     5581 tctctggggg attacctcga ccccgaactc atctctcgtt gccttgccga atcaggtact
     5641 gtaacgctac gcaagcgccg tcttcccctc gaaatgatgg tctggtgtat tgttggcatg
     5701 gcgcttgagc gtaaagaacc tcttcaccag attgtgaatc gcctggacat catgctgccg
     5761 ggcaatcgcc ccttcgttgc cccccagtgc cgttattcag gcccgccagc gcctgggaag
     5821 tgaggctgtc cgccgcgtgt tcacgaaaac agcgcagctc tggcataacg ccacgccgca
     5881 tccgcactgg tgcggcctga ccctgctggc catcgatggt gtgttctggc gcacaccgga
     5941 tacaccagag aacgatgcag ccttcccccg ccagacacat gccgggaacc cggcgctcta
     6001 cccgcaggtc aaaatggtct gccagatgga actgaccagc catctgctga cggctgcagc
     6061 cttcggcacg atgaagaaca gcgaaaatga gcttgctgag caacttatag aacaaaccgg
     6121 cgataacact ctgacgttaa tggataaagg ttattactca ctgggactgt taaatgcctg
     6181 gagcctggcg ggagaacacc gccactggat gatacctctc agaaagggag cgcaatatga
     6241 agagatcaga aaactgggta aaggcgatca tctggtgaag ctgaaaacca gcccgcaggc
     6301 acgaaaaaag tggccgggac tgggaaatga agtgacagcc cgcctgctga ccgtgacgcg
     6361 caaaggaaaa gtctgccatc tgctgacgtc gatgacggac gccatgcgct tccccggagg
     6421 agaaatggcg gatctgtaca gtcatcgctg ggaaatcgaa ctgggataca gggagataaa
     6481 acagacgatg caactgagca ggctgacgct gagaagtaaa aagccggagc ttgtggagca
     6541 agagctgtgg ggtgtcttac tggcttataa tctggtgaga tatcagatga ttaaaatggc
     6601 ggaacatctg aaaggttact ggccgaatca actgagtttc tcagaatcat gcggaatggt
     6661 gatgagaatg ctgatgacat tgcagggcgc ttcaccggga cgtataccgg agctgatgcg
     6721 cgatcttgca agtatgggac aacttgtgaa attaccgaca agaagggaaa gggccttccc
     6781 gagagtggta aaggagaggc cctggaaata ccccacagcc ccgaaaaaga gccagtcagt
     6841 tgcttaactg atcggcatta cagcccttga gctgccgttt ttttattctg ttagttgtga
     6901 aactgaagcg atttagtcac tatcgatctc atcaaatatg gctcgctttg agatattcct
     6961 caagtaaaaa aacacctctt cctgcgattt ctcacaaaaa agattcgttg acaaaaagtg
     7021 acaaaattat gagatttcca tcacacattt tgacatcagg aacggtatgc tgaattcacc
     7081 aagacgggaa gacaagaggt aaaatttatg acaatgaaca ttaccagcaa acaaatggaa
     7141 attactccgg ccatccgcca acatgtcgca gaccgtctcg ccaaactgga aaaatggcaa
     7201 acacatctga ttaatccaca tatcattctg tccaaagagc cacaagggtt tgttgctgac
     7261 gccacaatca atacacctaa cggcgttctg gttgccagtg gtaaacatga agatatgtac
     7321 accgcaatta acgaattgat caacaagctg gaacggcagc tcaataaact gcagcacaaa
     7381 ggcgaagcac gtcgtgccgc aacatcggtg aaagacgcca acttcgtcga agaagttgaa
     7441 gaagagtagt cctttatatt gagtgtatcg ccaacgcgcc ttcgggcgcg ttttttgttg
     7501 acagcgtgaa aacagtacgg gtactgtact aaagtcactt aaggaaacaa acatgaaaca
     7561 cacaccgttt ttcttcgcat tcttttttac cttcccctga atgggaggcg tttcgtcgtg
     7621 tgaaacagaa tgcgaagacg aacaataagg cctcccaaat cggggggcct tttttattga
     7681 taacaaaaag gcaacactat gacatcggaa aacccgttac tggcgctgcg agagaaaatc
     7741 agcgcgctgg atgaaaaatt attagcatta ctggcagagc gacgcgaact ggccgtcgag
     7801 gtgggaaaag ccaaactgct ctcgcatcgc ccggtacgtg atattgatcg tgaacgcgat
     7861 ttgctggaaa gattaattac gctcggtaaa gcgcaccatc tggacgccca ttacattact
     7921 cgcctgttcc agctcatcat tgaagattcc gtattaactc agcaggcttt gctccagcaa
     7981 catctcaata aaattaatcc gcactcagca cgcatcgctt ttctcggccc caaaggttct
     8041 tattcccatc ttgcggcgcg ccagtatgct gcccgtcact ttgagcaatt cattgaaagt
     8101 ggctgcgcca aatttgccga tatttttaat caggtggaaa ccggccaggc cgactatgcc
     8161 gtcgtaccga ttgaaaatac cagctccggt gccataaatg acgtttacga tctgctgcaa
     8221 cataccagtt tgtcgattgt tggcgagatg acgttaacta tcgaccattg tttgttggtc
     8281 tccggcacta ctgatttatc caccatcaat acggtctaca gccatccgca gccattccag
     8341 caatgcagca aattccttaa tcgttatccg caatggaaga ttgaatatac cgaaagtacg
     8401 tctgcggcaa tggaaaaggt tgcacaggca aaatcaccgc atgttgctgc gttgggaagc
     8461 gaagctggcg gcactttgta cggtttgcag gtactggagc gtattgaagc gaatcagcga
     8521 caaaacttca cccgatttgt ggtgttggca cgtaaagcca ttaacgtgtc tgaccaggtt
     8581 ccagcgaaaa cgacgttgtt aatggcgacc gggcaacaag ctggtgcgct ggttgaagcg
     8641 ttgctggtac tgcgcaacca caatctgatt atgacccgtc tggagtcacg cccgattcac
     8701 ggtaatccat gggaagagat gttttatctg gatattcagg ccaatcttga atcagcggaa
     8761 atgcaaaaag cattgaaaga gttaggggac atcacccgtt cgatgaaggt attgggctgt
     8821 tacccaagtg agaacgtagt gcctgttgat ccaacctgat gaaaaggtgc cggatgatgt
     8881 gaatcacccg gcactggatt attagtgacg gttgtcattc gcctgacgca ataacacgcg
     8941 gctttcactc tgaaaacgct gtgcgtaatc gccgaaccag tgctccacct tgcggaaact
     9001 gtcaataaac gcctgcttat cgccctgctc cagcaactca atcgcctcgc cgaaacgctt
     9061 atagtaacgt ttgattaacg ccagattacg ctctgacgac ataataatgt cggcatacag
     9121 ttgcggatcc tgggcaaaca gtcgcccaac catcgccagc tcaaggcggt aaatcggcga
     9181 agaaagcgcc agaagttgct caagctgaac attttcttct gccaggtgca gcccataagc
     9241 aaaagtagca aagtggcgca gtgcctgaat aaacgccata ttctgatcgt gctcgacagc
     9301 gctaatacga tgcagccgag cgccccagac ctgaatttgc tccagaaacc attggtatgc
     9361 ttccggctta cgtccatcac accagaccac aacttgtttt gccaggctac cgctgtccgg
     9421 gccgaacatc gggtgtaacc ccagcaccgg accatcatgc gccgccagca tggcctgtaa
     9481 tggtccattt ttcaccgatg ccagatcgac cagaatacaa tctttcggta aaggcggtaa
     9541 tttgccaata acttgttcag taacgtggat gggcacacta acaatcacca ttccggcatc
     9601 ggcaactatc tcagccgctc gatcccagtc atgttgctcc agaatccgca cctgataacc
     9661 cgatagtgtc agcatcttct cgaacaggcg tcccatctga ccgccaccgc cgacgataac
     9721 caccgggcgc agtgacggac aaagcgtttt aaatcctttg tcgttttcac tggagtaaga
     9781 ttcacgcatc acccgacgca aaacatcctc aatcagatct ggcggtacac ccagagcttc
     9841 cgcctctgca cgacgcgagg ccaacataga tgcctcgcgc tccggaacat aaataggcag
     9901 tccaaagcgg cttttcacct cgcccacttc agcaaccagt tccagacgct tcgctaataa
     9961 attcagcagc gctttatcga cttcatcaat ttgatcgcgt aatgcggtca attcagcaac
    10021 cataataaac ctcttaagcc acgcgagccg tcagctgccc gttcagatcc tgatgaattt
    10081 cacgcagcaa ggcatcggtc atttcccagc taatgcaggc atcggttacg gatacaccgt
    10141 atttcatttc actgcgcggt tgctcggaag actgattgcc ctcgtggata ttactttcga
    10201 tcatcagacc aataattgag cgattgccat ctttgatttg agcaaccacg gattctgcca
    10261 ccgcaggctg acggcgataa tctttattgg aattaccgtg gctgcaatct accatcagag
    10321 acgggcgcag tcccgcctgt tccatctctt tttcacattg cgcaacatcc gcagggctat
    10381 agttcggcgc tttaccaccg cgcaggatca catgcccgtc cggattcccc tgagtttgta
    10441 gcaacgccac ctgccctgcc tggttaatgc caacaaaacg gtgcggctgg gcggcggcac
    10501 gcatagcgtt aatagccgtt gcaaggctgc cgtcggtgcc gtttttaaaa ccaaccggca
    10561 tggaaagccc ggaggccatt tcacggtgtg tttgcgattc cgttgtacga gcaccaattg
    10621 ctgaccagct aaacagatcg cccaggtatt gcgggctatt aggatctaac gcttccgtcg
    10681 ccagtggcag tcccatattc accagctcaa gcagcaattt acgcgcgatc tgcagcccgg
    10741 cttctacatc aaaagagcca tccatatggg gatcgttaat caaccctttc caaccgacag
    10801 tggtacgggg tttttcaaaa tagacgcgca ttaccagata gaggctatcg ctgacctctg
    10861 cggcaagggc tttaaatcga cgagcatatt ccagagcagt ttccggatca tgaatggaac
    10921 aaggaccaca gactaccaac agacgaggat cgcgcccggc gataatatct gaaatggttt
    10981 tacgcgagtc agcaatctgg gcttcctgtt gcaggcttaa tggaaaagcg gccttcagtt
    11041 gttccggagt cattaaaact tgttcgtcgg taatatgtac gttattcagc gcgtcttttt
    11101 gcatgatggc gatcctgttt atgctcgttt gcgatagttg atcctcagcg aggatgacgt
    11161 aacgataaca cataaagtaa agttttcaat ccatatttcg tacatttttc tttacacaag
    11221 caatttattc gcgcttttaa cccttgttta tgtataaata aatttacacc acctttgaaa
    11281 acaatccgct atgctttgaa aaaggagaaa gaaatgatga aaaagtttat tgcccccttg
    11341 ttggctttac tggttagcgg atgtcagatt gatccttata ctcacgcgcc aaccttgacc
    11401 agcaccgact ggtatgatgg cggtatggaa gatgcgatat cgggcagcac cataaaagat
    11461 gacgatgcat ttagcgattc acaggcggat cgcggtctat accttaaagg atatgccgaa
    11521 ggacaaaaga aaacttgcca gaccgatttt acttatgccc gaggactttc cggtaaaagc
    11581 tttcctgcca gctgcaacaa tgttgaaaat acaagccaac tgcatgaagt atggcaaaaa
    11641 ggggctgatg aaaatgccag cacaatacga ttaaattaat taaaacccca caaataaatt
    11701 aaacataaga ttttactcat tatgttttat ttctcacaat gatgacgatc gcatgaaaaa
    11761 atgatatttt aatgagcatt ctgaaaaaat atcgatgcat ttcgagcgaa gatggtgggg
    11821 atccctgaat gcgtttttct caccgactgt tcttacttct gattctcctg ctgacgggag
    11881 caccaatact cgctcaggag ccttctgacg ttgcgaaaaa cgtgcgtatg atggtttccg
    11941 gtattgtcag ttacacccgc tggcctgcgc tatcagggcc accaaagtta tgtatctttt
    12001 catcttcacg ttttagtacc gccctacaag agaatgccgc gacatctttg ccttatctgc
    12061 ctgtcatcat tcacactcaa caagaggcga tgatttcagg ctgcaatggt ttttattttg
    12121 gcaatgagtc accgacattc cagatggaat taaccgaaca atatccgtca aaggcgttat
    12181 tattaattgc cgaacaaaat accgagtgca ttattggtag cgccttttgt ctgatcatcc
    12241 acaataatga cgtcagattt gccgtaaacc tggatgcctt atcgcgtagc ggtgtaaagg
    12301 tcaacccgga tgtcttgatg ctcgcacgga agaaaaatga tggataacga taattctctt
    12361 aataagcgcc ccacgtttaa aagagcatta cgcaacatca gtatgaccag catatttatc
    12421 actatgatgc tgatctggtt gctgctttcc gtgacctcgg tgctgaccct gaaacagtac
    12481 gcgcaaaaaa acctggcact gacagcagca acaatgactt acagtctgga agcagcggtc
    12541 gtttttgccg atggccctgc agcaactgaa acactggcag cgctgggcca gcaagggcaa
    12601 ttttcaactg cagaagtacg tgataagcag caaaatattc tggcatcctg gcattacacc
    12661 cgtaaggatc caggcgatac tttcagcaat ttcataagcc actggctctt ccctgccccc
    12721 atcattcagc cgattcgtca caatggcgaa accattggcg aagtacgctt aaccgctcgc
    12781 gacagttcaa tcagccattt catctggttt tcgctcgccg tactgaccgg ttgtattctg
    12841 ctggcatcag gaatcgcaat taccctcacc cgccatttgc acaatggcct ggtggaagca
    12901 ctgaaaaata tcaccgatgt cgtacatgat gtgcgttcca accgcaattt ttcccgacga
    12961 gtttcggaag aacgtatcgc tgagtttcac cgcttcgctc tcgacttcaa cagtctgctt
    13021 gatgaaatgg aagagtggca gcttcgttta caggccaaaa atgcgcagct tctgcgtacc
    13081 gcgctacatg acccattaac cgggctggct aaccgcgcag cgtttcgtag cggcatcaac
    13141 acattgatga gcagttccga cgcccgaaaa acgtcggcgt tactatttct tgatggcgat
    13201 aatttcaaat atatcaatga tacctggggt catgcgacgg gcgatagagt cttgattgaa
    13261 atcgcaaaac ggttagctga atttggcggg ctgcgacata aagcataccg cctgggcggc
    13321 gatgaattcg ctatggtgct ctatgatgta cagtcggaat ctgaagtgca gcagatatgc
    13381 tcagcactga cacaaatctt taatctcccg tttgatcttc ataatggcca tcagaccacc
    13441 atgacattaa gcattggtta cgcgatgacc attgagcacg cctctgcgga aaaattacaa
    13501 gagcttgccg atcacaatat gtatcaggcc aaacaccagc gtgccgaaaa gctggtgaga
    13561 taacaaggat atatcgatat gataaagcac ctggtagcac ccctgatttt cacctcgcta
    13621 atactgactg gctgccagtc ccctcaggga aagtttactc ctgagcaagt cgccgctatg
    13681 caatcttatg gatttactga atccgccggc gactggtcgc tgggcttatc agatgccatt
    13741 ctgttcgcaa aaaatgacta caaattgctc ccggaaagcc agcaacagat ccaaaccatg
    13801 gcagccaaat tggcctcgac agggctaaca catgcccgta tggatggaca caccgataac
    13861 tatggtgaag acagttacaa cgaaggctta tcattgaaac gggcgaatgt cgtggccgat
    13921 gcatgggcta ttggtggaca aattccacgc agcaatctca ccacacaggg tttaggaaaa
    13981 aaatatccca tagccagtaa caagaccgcc cagggccgcg ccgagaaccg ccgcgtcgca
    14041 gtggtgatta caacccctta aaacacgagc aaacagacaa aaaaagggcc agccaattgg
    14101 ccagcccttc ttaacaggat gtcgcttaag cgaaatctta gttaagacgc tctttgatac
    14161 gagcagcctt accagtacgc tcacgcaggt agtacagttt agctttacga acagcaccac
    14221 gacgtttgac agaaatgctg tcaactaccg gagagtgagt ctggaagaca cgctcaacgc
    14281 cttcgccgtt ggaaatttta cgaacagtga atgcagagtg cagaccgcgg ttacgaatag
    14341 cgataaccac gccctcgaat gcctgcagac gttttttgga accttcaaca acccatactt
    14401 tcacttccac ggtatcaccc ggacggaagg aaggtacgtc ctgcttcatc tgctcttgtt
    14461 caagttgctt aataatgttg ctcataattt aatctcttat cctgggtaaa ctgatatctc
    14521 gggggcttac gccatcccat catgtttatg ttgctgttgt gcgtgttccg ttttgaactc
    14581 cgccagcaac cttgcttgct cttcagtcag agccaggttt tccagaagtt caggtcttct
    14641 aagccaggta cggcccagcg actgtttcaa acgccagcga cgtatctcgg catggttgcc
    14701 cgacagtaac actggcggaa cttccatccc ttctaacacc tcaggccgcg tatagtgcgg
    14761 gcaatccagc aatccttcag caaaggaatc ttccgttgcc gaggcttcat gtcccagtac
    14821 tcccggaata aaccgggaaa cggagtcaat cagcgtcatt gctggtaact caccaccact
    14881 gagaacgtaa tcgccgattg accattcttc gtcaatttcg gtttggatca cgcgctcatc
    14941 tataccttcg tagcgaccgc acaccagaat caatttttga ttcgttgcca gttcgctgac
    15001 gcccgcttga tcaagcttgc gtccctgtgg tgacagataa atcacctttg cgccttcacc
    15061 cgccgcggct tttgctgcat gaatggcgtc ccgcaagggt tgcaccatca ttaacatccc
    15121 cggtccgccg ccgtaaggac gatcgtccac ggtacggtgc cggtcatgcg tgaagtcgcg
    15181 aggactccaa ctctggatgc tcagcaggcc atttttaact gcccggccag ttaccccgta
    15241 atcggtaatt gcgcggaaca tttcaggaaa caggctaatt atgccaatcc acatagcgcc
    15301 gtcttttacc gtttatccgg tggtttaaaa accaggatcc caatctactt cgattgaacg
    15361 agtagtgaga tcgactttct tgataacctg cccatcgagg aacggtacga gacgttcctt
    15421 gataccaaac gcatctttca ggtttgcctt aatgacgaga acgtcattag atccggtttc
    15481 catcatatcg acgactttac cgagatcgta gccttcagtg gttactacct ggcagcccat
    15541 caggtctttc cagtagtagt caccctcttc aagctgaggc agctgcgatg aatccacgac
    15601 aatttcacaa ttcgtcagca ggttcgccgc atcacgatcg tcaacgcctt tcagcttgat
    15661 gatcatgtcc tgattgtggt gcttccagct ttccagctgg acttgctgcc actgacccgc
    15721 cttctggata aaccagggct gatagtcaaa aatgctttcg gcgtcttcgg tggaagaaaa
    15781 cactctgagc cacccacgaa taccgtaaga cgaacccatt tttcccaaaa cgatgggatc
    15841 aacaggtgct tgcgcggtga gttgtttgct catcatgacc accgtgacag attaagctgc
    15901 tttgtttact tctttgatca gcgcagcaac gcgatcagaa atagttgcgc cctggccaac
    15961 ccagtgagcg atgcgatcca gatccaggcg agtgccttct tctttttcgc tagcgattgg
    16021 gttgaagaaa ccaacgcgct cgatgaagcg accgttgcgt gcattacggc tgtcagcgac
    16081 aacaacctgg tagaacggac gctttttagc gccgtgacgt gctaaacgaa tagttaccat
    16141 aacatcctct tgtgtgaata aaacaaccgg accccatcga ggaacggagt ccggtgtcat
    16201 attaaaagcc cgaaaatttt actcattttt gcagaaattg caatcaacag ttgctaactc
    16261 tgctgtaaaa ggccgtcggc ggtgcagcca gtttggtgcc ggagtgcgcg cagtcaccgg
    16321 agcgtacacg caatacgtga ggatgacgag cacaccccgg tgccaaaatg gcaaacaagc
    16381 caggccgatt agcgaccagg aaatcccggc ggcatcatac ccttcatgct tctcatcatc
    16441 ttcgccatcc cgcccttctt cattttcttc atcatgcgct gcatgtcgtc gaactgtttc
    16501 agaagacggt taacgtcctg cacctgcata ccgcaaccgg cagcgatacg gcgtttacgc
    16561 gaacctttga tgatttctgg cttagcgcgc tctttcatcg tcatcgagtt gatgatggct
    16621 tccatacgca ccagcacttt atcgtccatc tgcgacttga cgttatccgg gatctgcccc
    16681 atgcccggca gtttgcccat caagctggcc atgccgccca tatttttcat ctggcgcagt
    16741 tgctcaagga agtcgttgag atcgaagccg tcgccttttt ttaacttgct ggctaatttc
    16801 tctgcctgcg cgcggtcaac tttgctttcg atatcttcga tcagtgacag tacgtcgccc
    16861 atgccgagaa tacgcgacgc gatgcggtcc ggatggaacg gctccagcgc ctcagttttc
    16921 tcgccaacac cgaggaactt gatcggtttg ccagtgatgt gacgaataga gagcgccgca
    16981 ccgccgcggg catcgccgtc cactttggtc aacactacgc cggtaagcgg taacgcttca
    17041 ttgaatgctt ttgccgtatt ggccgcatcc tgaccggtca tggcgtcaac cacaaatagg
    17101 gtttcaaccg ggttaatcga cgcatggact tgtttgatct cgtccatcat cgcttcgtca
    17161 acgtgcagac gaccagcggt atccaccagc agcacgtcgt agaatttcag tttggcttct
    17221 ttcagcgccg cgttaacgat atctaccggc ttctgaccaa catcagaagg gaagaaatca
    17281 acgcccacct gctctgccaa cgtctcaagc tgtttgattg ccgccgggcg ataaacgtcg
    17341 gcagaaacca ccagcacttt cttcttgtgc ttctcgcgca ggaacttacc gagtttacca
    17401 acgctggttg ttttaccggc accttgcagg cccgccatca gcacgaccgc aggcggttgc
    17461 gcagccaggt tcagggtctg gttctcttcg cccatcgccg caaccagttc gttacggacg
    17521 attttgacga actcctgccc cggcgtcagg ctcttattaa cttcatgacc aaccgctttc
    17581 tcttttacgc gattgataaa ctcacgcact accggcagag cgacgtccgc ctccagcagc
    17641 gccatgcgca cttcgcgcag cgtatctttt acgttgtctt cagtgaggcg tccacggcca
    17701 ctgatattgc gcagcgtgcg cgacaaacga tcggttaaat tatcaaacat tgtctctcgc
    17761 ctggggtgga aacggttggc cgcaatcgcg acacatcatc agtattttgc cgcagtataa
    17821 catgaaggcg tctttgttgt tatgcaacgg ttggagcagc gttcacctga cgctatactg
    17881 cttctctttc ttattgctca aactgtcgac attactatgc ccgtttttgc tctgctcgcg
    17941 cttgtcgcct actccgtcag tcttgcgctg attgttcccg gtctgctgca aaaaaacggc
    18001 ggctggcggc gcatggctat tatttctgcg gtcattgcgc tggtctgcca cgcaatcgct
    18061 ctggaagccc gcatcctgcc cgacggtgat agcggacaaa acctcagcct gctgaacgtt
    18121 ggttcattgg tcagtttgat gatctgtacg gtaatgacca ttgtggcttc tcgcaatcgt
    18181 ggctggttgt tgctacccat tgtctatgcc tttgcgctta tcaacctggc gctggcgacc
    18241 ttcatgccca atgaatacat cacccatctg gaagcaacgc cggggatgct ggtgcacatt
    18301 ggcttatcgc ttttttccta tgccacgcta attatcgctg ccctgtacgc gctgcaactg
    18361 gcgtggattg attaccaact gaagaacaag aagctggcat ttaaccagga aatgccgcca
    18421 ttgatgagta tcgagcgtaa aatgttccat atcacgcaga ttggcgtggt gctgctaacg
    18481 ctcactcttt gcactggcct gttctacatg cacaacttat ttagcatgga aaatatcgac
    18541 aaggccgtgc tctctatcgt ggcgtggttt gtctatattg tgctgctgtg gggacattat
    18601 catgaaggat ggcgtggacg ccgcgtcgtc tggtttaacg ttgcgggtgc agtcattctg
    18661 acactggcct acttcggcag ccgaattttt cagcagttaa tcagctaaac ccagaaaagg
    18721 agtttcccct ggaacacatt tctactacta cgttgatcat tattctgatc atcatggtgg
    18781 tcatttcagc ctatttttcc gggtccgaaa ccggaatgat gactctcaac cgctatcgtc
    18841 ttcgacatat ggcgaaacag ggtaatcgct cggccaaacg cgtcgaaaaa ttgctgcgta
    18901 agccagaccg cctgataagc ctggtgttaa tcggcaataa cctggtcaat attcttgcct
    18961 ccgcgctcgg cactattgtt gggatgcgtt tgtacggcga tgcgggcgtg gcaattgcga
    19021 ctggtgtgct gacttttgtc gtgctggttt ttgctgaggt attgccgaaa accattgccg
    19081 cgctgtaccc ggaaaaagtc gcttatccga gtagtttttt actggctccg ctgcaaattt
    19141 tgatgatgcc gctggtctgg ttgctgaatg ctattacccg catgttaatg cgcatgatgg
    19201 gcatcaaaac cgatatcgtg gttagcggct ctttgagcaa agaagagttg cgtactatcg
    19261 tgcacgaatc acgctcacaa atttcccgtc gcaatcagga tatgctgctg tcggtgctcg
    19321 atctggaaaa gatgaccgtc gatgacatca tggtgccgcg aagtgaaatt atcggtattg
    19381 atatcaacga tgactggaaa tcgattctgc gccaactctc ccactcacct cacgggcgca
    19441 tcgtgctcta ccgtgattcg ctggacgacg ccatcagtat gctgcgtgtg cgtgaagcct
    19501 ggcgactcat gtcggagaaa aaagagttca ccaaagaaac catgctacgc gccgcggacg
    19561 agatctattt tgtcccggag ggtacgccgc tcagcacaca gctggtcaag tttcagcgca
    19621 acaaaaagaa agtcggcctg gtcgtcaacg aatatggaga cattcagggg ctggtgacgg
    19681 ttgaagatat tctggaagag attgtcggcg atttcactac ctcgatgtcg ccaacacttg
    19741 ccgaagaggt cacgccgcaa aacgacggtt cggtgattat cgatggcacc gccaacgtgc
    19801 gagaaatcaa caaagccttt aactggcatc taccggaaga tgatgcccgc acggttaatg
    19861 gcgtcattct tgaggcactg gaagagatcc ctgtcgcagg cacccgcgtg cgtattggcg
    19921 agtacgatat cgatattctc gacgtacagg acaatatgat taagcaggta aaagtttttc
    19981 ctgtaaaacc gctgcgcgag agtgtggcgg agtaacgaaa acggccctgc attcgcatgc
    20041 agggccgtga attattacga aaacagaaat tacgcttttg ctttcgctac agttaccatc
    20101 gccgcacgaa tcgtacgacc attcagcgta taacccttct gcataatgcc cagtacgtta
    20161 cctggcgcaa cgtcatcaga ttccaccatt gcgatggcct gatgcacatt cgggtccagc
    20221 gggacgttag tttcggcgat cacttcaacg ccaaacttac gcacaacatc cagcatcgac
    20281 ttcagcgtca gctcaatacc ttcaaccatc gcggacatat ccgggttagc tttatcagcc
    20341 acttccagcg cacgatccag gctatcaatc accggcagca attcgttgat gaatttctcc
    20401 agcgcgaatt tgtgggcttt ttcaatatcc agttcagtac gacgacgcag gttttccatt
    20461 tcggctttta cgcgcaaaat gccgtcacgt tcacgagtct gggcttcagc cagctgagct
    20521 tcgagattcg caattttttc atcgcgcgga tccacctgct cagcagaagc gtctggctca
    20581 actgcctcaa tctcttcgtg ctgatccatg ataatttctt ccggggcttg cccctcaggc
    20641 gttttctgtt ctttactact catgaatttc tccgcgtttt tttcgcattc atctcgctaa
    20701 cttcgcttat tatggggatc agtttcaggg tttcaaggga agcactcaca ttgtcatcaa
    20761 tcttcgcaac aaggacctcg gaaaaatgaa taatcatttc aagtgtattg gcattgtggg
    20821 acacccacgg caccccactg cactgacaac acatgaaatg ctctaccgct ggctgtgcac
    20881 aaaaggttac gaggtcatcg ttgagcagca aatcgctcac gaactgcaac tgaagaatgt
    20941 gaagactggc acgctcgcgg agattgggca actggctgat ctcgcggtag tcgttggtgg
    21001 cgacggtaat atgctgggcg cggcgcgcac actcgcccgt tacgatatta aagttattgg
    21061 aatcaaccgt ggcaacctgg gtttcctgac tgaccttgac cccgataacg cccagcaaca
    21121 attagccgat gtgctggaag gccactacat cagcgagaaa cgttttttgc tggaagcgca
    21181 agtttgtcag caagattgcc agaaacgcat cagcaccgcg attaacgaag tggtacttca
    21241 tcctggcaaa gtggcgcata tgattgagtt cgaagtgtat atcgacgaga tctttgcgtt
    21301 ttctcagcgt tccgatggac tgattatttc gacgccaaca ggctccaccg cctattccct
    21361 ctctgcgggc ggtcctattc tgaccccctc tctggatgcg attaccctgg tgcccatgtt
    21421 cccgcatacg ttgtcagcac gaccactggt gataaacagc agcagcacga tccgtctgcg
    21481 tttttcgcat cgccgtaacg acctggaaat cagttgcgac agccagatag cactgccgat
    21541 tcaggaaggt gaagatgtcc tgattcgtcg ctgtgattac catctgaatc tgattcatcc
    21601 gaaagattac agttatttca atacattaag caccaagctc ggctggtcaa aaaaattatt
    21661 ctaattttac gccagcctct ttactgtata taaaaccagt ttatactgta cacaataaca
    21721 gtaatggttt ttcatacagg aaaacaacta tgttggcaca actgaccatc agcaactttg
    21781 ctatcgttcg tgagcttgag attgattttc atagcggcat gaccgtaata actggcgaga
    21841 ccggcgcggg taaatctatt gcaatagatg ccctcggcct ttgtctcggt ggtcgcgctg
    21901 aagccgacat ggtgcgtacc ggcgctgctc gtgccgacct gtgcgcccgt ttttctctga
    21961 aagatacgcc agcggccctg cgctggctgg aagaaaacca gcttgaagac ggtcatgaat
    22021 gtttgcttcg tcgcgtaatc agtagcgatg gtcgctcacg aggttttatc aacggtacag
    22081 ctgttcctct gtcacaactt cgcgaactgg gccagttgct gattcagatc catggtcagc
    22141 acgcccatca gttactcacc aaacccgagc accaaaaatt cctgcttgat ggctatgcca
    22201 atgaaacctc tctgctccag gaaatgaccg cacgttatca gttgtggcat caaagctgcc
    22261 gtgacctcgc gcatcatcaa cagttaagtc aggaacgcgc cgcccgtgcg gaactgctgc
    22321 aataccaatt aaaagaactt aacgaattta atccgcagcc cggagagttt gagcaaatcg
    22381 acgaagagta caaacgtctg gcgaacagcg gtcaattgct gactaccagc cagaatgcat
    22441 tggcagtaat ggccgacggt gaagacgcaa acctgcaaag tcagctttac acggctaaac
    22501 aactggtgag cgaattgatt ggcatggaca gcaaactgtc cggcgtactt gatatgctgg
    22561 aagaagctac catccagatt gctgaagcca gcgatgaact gcgccactac tgcgatcgtc
    22621 tggatctcga tcccaaccga ctgtttgaac ttgaacagcg catctcaaaa cagatttcgc
    22681 tggcacgtaa acatcacgtc agccctgagg cattgccaca gtattaccag tcgctactgg
    22741 aagaacagca gcaactggac gatcaggccg actcacaaga aacgctcgcg ctggcggtaa
    22801 cgaaacatca tcagcaggca ctggaaaccg cgcgcgcatt acaccagcaa cgccagcagt
    22861 atgcagaaga acttgcactg ctgatcaccg acagtatgca tgcgctctca atgccgcatg
    22921 ggcagtttac gatcgatgtt aaatttgacg agcatcacct gggcgctgac ggtgccgatc
    22981 gtattgagtt tcgggtaacc accaacccag gtcagccaat gcagcctatt gccaaagtcg
    23041 catccggtgg tgaattgtca cgcatcgcat tggcaattca ggtcatcacg gcgcgtaaaa
    23101 tggaaacccc ggcactgatt tttgatgaag tggatgtagg gattagcggt ccaacagcgg
    23161 cagttgtcgg caaactgctg cgtcaacttg gcgaatcaac tcaggtgatg tgtgttaccc
    23221 acctgccaca agtcgcggga tgtggtcatc aacactattt tgtcagcaaa gaaaccgatg
    23281 gtgcgatgac agaaacacac atgcaatccc tggataaaaa agcgcggtta caagagctgg
    23341 cgcgcctgct tgggggcagt gaagtcacac gtaacacact ggcgaatgcg aaagaactgc
    23401 ttgcggcgta aacttttttc ctgcttcacg gtcagagtaa acagcaaaac gccgtaagac
    23461 cggaaagcaa aaggttttaa agtgatgaaa ggtctattat catcggcata ttacagatga
    23521 gccacgtact gctcgggccc gaaaaggaat caaatcacta tgcgctgtaa aacgctgact
    23581 gctgcagcag cagtactatt gatgttgacc gcaggctgtt ccactctgga gcgagtggtt
    23641 taccgtcctg acatcaacca ggggaattat ctgaccgcta acgacgtatc caaaatacgc
    23701 gttggcatga cgcaacaaca agttgcgtac gcattgggta caccgctgat gtccgatcca
    23761 tttggtacga atacctggtt ctatgtcttc cgccagcaac caggtcatga aggtgtaacg
    23821 cagcaaacgc tgacgctgac ctttaatagt agcggggtgt tgaccaatat tgataataaa
    23881 cctgcgctga gtggtaacta atcataaagt tgctctcaaa gacgttaaaa aaggtgctca
    23941 atgagcacct tttttctgtc tgttatttat tcgctgattt ttctgctcgt tgcctgcgca
    24001 gctctttcgg atcggcaatg agagggcgat aaatctccac ccgatcgcca tcatgcacac
    24061 tatcgctcag ttttgccgga cggctataaa tgccgacttt gtttttagtt aaatcgatat
    24121 cggtacgcaa ttccagcaag ccactggcgc gaatagcttc ttcaaccgtc gcaccctcct
    24181 gcagcgtcac acgctgcagg tactgcttct caggtagcgc ataagccacc tcaacggcaa
    24241 ttttacctgg cactgtaaac ctctttcgca cgaaccgtaa aagcctggac catattagcc
    24301 gccagttctt taaacacgcg gccaaaggcg agttcaatca acttattggt aaactcaaaa
    24361 tcgagatgaa actcgatacg gcacgcctcc tgactcagcg gcgtaaactt ccatccaccg
    24421 atcaatttct tgaacggccc atccaccaga ttcataagaa tactttggtt actggtcaac
    24481 tggttgcggg tagtaaacgt tttgctgatc ccagccttag agacatctac cgccgcagtc
    24541 atctgcccag gagtggactc cagaatccga cttccggtac aacccggcaa aaactgagga
    24601 taagactgaa cgtcattcac taactgatac atttgctccg cgctgtaggg taccagtgcg
    24661 gtccggctaa tctgaggcat aacaatttcc atcaacaaaa atccaacaaa taatatcatt
    24721 tatcctgctg taaaaaaaac gctatcccgg cgctgggtaa catcgggttc atgctaagat
    24781 agagccttgt cccccgcagg attgatatgg ggtgttttcg atttcagatt accgatgatt
    24841 cacgacgctt atgacgaaga aaaaagcaca taaacctggt tcagcgacca tcgcgcttaa
    24901 caagcgcgcc cggcacgaat actttatcga agaagagttc gaggcgggac ttgccctgca
    24961 aggctgggag gttaaatccc tgcgtgcagg aaaagccaat atcagcgaca gctacgtcct
    25021 tctgcgtgac ggagaggcat ttctgtttgg cgctaacatc acgccaatgg ctgtggcctc
    25081 cacgcatgtg gtgtgcgatc ctacccgtac ccgcaagtta cttctcaacc agcgcgaact
    25141 ggactcattg tacggtcgcg tcaatcgaga aggctatacc gtagtggcgc tctccctgta
    25201 ctggaaaaat gcctggtgca aagtgaaaat cggcgtcgcc aaaggtaaga aacagcatga
    25261 taaacgttca gatatcaaag aacgcgaatg gcaggtggat aaagcgcgta tcatgaaaaa
    25321 cgcccaccgt taaacctgca ctgcaattat tgatccgctc ctcaccgcgc ctccctctcc
    25381 ggcggcgcga atgcacatct tattggctat cacatccgac ccaaatgttg ccatcccatt
    25441 gcttaatcga ataaaaatca ggctacatgg gtgctaaatc tttaacgata acgccattga
    25501 ggctggtcat ggcactcatg aatctggtat acttaccgtt acacattggg gctgattctg
    25561 gattcgacgg gatttgcgaa acccaaggtg catgccgagg ggcggttggc ctcgtaaaaa
    25621 gccgcaaaaa atagtcgcaa acgacgaaaa ctacgcttta gcagcttaat aacctgctta
    25681 gagccctctc tccctagcct ccgctcttag gacggggatc aagagaggtc aaacccaaaa
    25741 gagatcgcgt ggaagccctg cctggggttg aagcgttaaa acttaatcag gctagtttgt
    25801 tagtggcgtg tccgtccgca gctggcaagc gaatgtaaag attgactaag catgtagtac
    25861 cgaggatgta ggaatttcgg acgcgggttc aactcccgcc agctccacca atcatgattg
    25921 gacggtgtaa ggactacacc aacaaaaaca ggaagttata agtctcagca ggacaccgac
    25981 cagacggtga ggagacaaaa aaggatacgc aacggagtcg cgactcccta tgacattaaa
    26041 gcccgctgat gcgggctttt ttattggtcc tgacccgccc tgaatagaat tcatccaatt
    26101 acagtccaga catatacagt atccctcagc tcagtatcag cttctcgaca aatggtcact
    26161 gttatttacc cgttgccaga aagagaataa tttgccccca tagccagcct tcccggaatc
    26221 tgtatattca ttaatattgt tatcgattcc atcaagtaac ataggttgga tgcgattgat
    26281 cttactcaca ttagtcgaca cggaactcag taagttgaac tctggaaaag cagtttaaat
    26341 agcatttaaa caaatgccac cagttcgaaa aag
//
LOCUS       DAPHCV010000073        26214 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.073, whole genome shotgun sequence.
ACCESSION   DAPHCV010000073 DAPHCV010000000
VERSION     DAPHCV010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.073"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..585)
                     /locus_tag="RH033_002978"
     CDS             complement(<1..585)
                     /locus_tag="RH033_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDV2992130.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGF"
     gene            complement(656..2770)
                     /gene="fusA"
                     /locus_tag="RH033_002979"
     CDS             complement(656..2770)
                     /gene="fusA"
                     /locus_tag="RH033_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDV2992131.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEEEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2867..3337)
                     /gene="rpsG"
                     /locus_tag="RH033_002980"
     CDS             complement(2867..3337)
                     /gene="rpsG"
                     /locus_tag="RH033_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDV2992132.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3434..3808)
                     /gene="rpsL"
                     /locus_tag="RH033_002981"
     CDS             complement(3434..3808)
                     /gene="rpsL"
                     /locus_tag="RH033_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDV2992133.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3934..4221)
                     /gene="tusB"
                     /locus_tag="RH033_002982"
     CDS             complement(3934..4221)
                     /gene="tusB"
                     /locus_tag="RH033_002982"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HDV2992134.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLAGQISNDIILIDYTDFVRLTVKHSSQMAW"
     gene            complement(4229..4588)
                     /gene="tusC"
                     /locus_tag="RH033_002983"
     CDS             complement(4229..4588)
                     /gene="tusC"
                     /locus_tag="RH033_002983"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HDV2992135.1"
                     /translation="MKRIAFVFSTVPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4588..4974)
                     /gene="tusD"
                     /locus_tag="RH033_002984"
     CDS             complement(4588..4974)
                     /gene="tusD"
                     /locus_tag="RH033_002984"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HDV2992136.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRCWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4974..5696)
                     /locus_tag="RH033_002985"
     CDS             complement(4974..5696)
                     /locus_tag="RH033_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV2992137.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5863..6675)
                     /gene="fkpA"
                     /locus_tag="RH033_002986"
     CDS             complement(5863..6675)
                     /gene="fkpA"
                     /locus_tag="RH033_002986"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDV2992138.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6896..7114
                     /locus_tag="RH033_002987"
     CDS             6896..7114
                     /locus_tag="RH033_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HDV2992139.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7163..7753)
                     /gene="slyD"
                     /locus_tag="RH033_002988"
     CDS             complement(7163..7753)
                     /gene="slyD"
                     /locus_tag="RH033_002988"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDV2992140.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7848..8048)
                     /locus_tag="RH033_002989"
     CDS             complement(7848..8048)
                     /locus_tag="RH033_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HDV2992141.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(8058..9863)
                     /gene="kefB"
                     /locus_tag="RH033_002990"
     CDS             complement(8058..9863)
                     /gene="kefB"
                     /locus_tag="RH033_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HDV2992142.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTHFSWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHTDT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9863..10414)
                     /gene="kefG"
                     /locus_tag="RH033_002991"
     CDS             complement(9863..10414)
                     /gene="kefG"
                     /locus_tag="RH033_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HDV2992143.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10545..12458
                     /locus_tag="RH033_002992"
     CDS             10545..12458
                     /locus_tag="RH033_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV2992144.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12458..13480
                     /locus_tag="RH033_002993"
     CDS             12458..13480
                     /locus_tag="RH033_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDV2992145.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PHWQRLELPDGDFVDLAWSEDPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNNLPIDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13474..13692
                     /locus_tag="RH033_002994"
     CDS             13474..13692
                     /locus_tag="RH033_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HDV2992146.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13746..14615
                     /locus_tag="RH033_002995"
     CDS             13746..14615
                     /locus_tag="RH033_002995"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HDV2992147.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14670..15074)
                     /locus_tag="RH033_002996"
     CDS             complement(14670..15074)
                     /locus_tag="RH033_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HDV2992148.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15376..16008
                     /gene="crp"
                     /locus_tag="RH033_002997"
     CDS             15376..16008
                     /gene="crp"
                     /locus_tag="RH033_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HDV2992149.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            16059..18149
                     /locus_tag="RH033_002998"
     CDS             16059..18149
                     /locus_tag="RH033_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDV2992150.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQVLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQDAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPTELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18216..19436)
                     /gene="argD/dapC"
                     /locus_tag="RH033_002999"
     CDS             complement(18216..19436)
                     /gene="argD/dapC"
                     /locus_tag="RH033_002999"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HDV2992151.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEEGVMVLNAGPDVMRFAPSLVVEDADIDKG
                     MHRFAHAVAKVIGA"
     gene            complement(19522..20085)
                     /gene="pabA"
                     /locus_tag="RH033_003000"
     CDS             complement(19522..20085)
                     /gene="pabA"
                     /locus_tag="RH033_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HDV2992152.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGVGVFKGLANPLTVTRYHSLVVEPDSLPECFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(20117..20719)
                     /locus_tag="RH033_003001"
     CDS             complement(20117..20719)
                     /locus_tag="RH033_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HDV2992153.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQNGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20709..20876)
                     /locus_tag="RH033_003002"
     CDS             complement(20709..20876)
                     /locus_tag="RH033_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HDV2992154.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20981..21553)
                     /gene="ppiA"
                     /locus_tag="RH033_003003"
                     /gene_synonym="rotA"
     CDS             complement(20981..21553)
                     /gene="ppiA"
                     /locus_tag="RH033_003003"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HDV2992155.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21824..23005
                     /gene="tsgA"
                     /locus_tag="RH033_003004"
     CDS             21824..23005
                     /gene="tsgA"
                     /locus_tag="RH033_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HDV2992156.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSIAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23267..25810
                     /gene="nirB"
                     /locus_tag="RH033_003005"
     CDS             23267..25810
                     /gene="nirB"
                     /locus_tag="RH033_003005"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HDV2992157.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSSKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25807..26133
                     /gene="nirD"
                     /locus_tag="RH033_003006"
     CDS             25807..26133
                     /gene="nirD"
                     /locus_tag="RH033_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HDV2992158.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYDARVKDG
                     VVQLRG"
BASE COUNT         6409 a         7088 c         6551 g         6166 t
ORIGIN      
        1 gaagccagcc agttccagga ttttcgcttc ccactctgcg tcgccttcca gcgctttcag
       61 agcagaacca cgaacgatcg gagtgtcgtc gcccgggaag tcgtactgag acagaagttc
      121 acgaacttcc atttcaacca gttccagcag ctcttcgtca tcaaccatgt cacatttgtt
      181 caggaacacg atgatgtacg gaacgcctac ctgacgaccc agcaggatgt gctcacgagt
      241 ctgcggcatc gggccgtcag tcgcagcaac taccaggatc gcgccgtcca tctgagcagc
      301 accggtgatc atgtttttaa catagtcggc gtgccccggg cagtctacgt gtgcgtagtg
      361 acgggtcggg gtgtcatatt caacgtgaga agtgttgatg gtgataccac gagctttttc
      421 ttccggcgcg ttatcgatct ggtcgaatgc acgagcagca ccgccgtagg ttttagccag
      481 tacggtggtg attgcagcgg tcagagtagt tttaccgtgg tcaacgtggc cgatagtacc
      541 aacgttaacg tgcggttttg tacgttcaaa tttttcttta gacacggcta tattccttac
      601 tatagtgctc tccccttcag gagagagcac gggactttgg tattaaccct taggcttatt
      661 taccacgggc ttcaattacg gcctgagcaa cgttactcgg cgcttcatca tacttcagga
      721 attccatggt gtatgatgca cgacctttgg tcagagaacg cagctgagtt gcatatccga
      781 acatttcaga cagcggtact tcagcgtgga tcttaacgcc agtaacttca gattcctgac
      841 ctttgagcat accacgacga cggctcaagt caccgataac gtcaccggtg ttctcttccg
      901 gagtttctac ttcaaccttc atgatcggct caagcagaac tggtttcgct ttcttaaagc
      961 cttctttaaa ggcgatagaa gcagccagtt taaacgccag ttcagaggag tcaacgtcat
     1021 ggtaagaacc gaagtgcaga cgaataccca tgtctactac cgggtagcct gccagcggac
     1081 ctgctttcag ctgttcctgg atacctttat caacggccgg gatgtattcg ccagggatta
     1141 caccaccttt aatgtcgttg atgaactcgt agcctttcgg gtttgaaccc ggctccagcg
     1201 ggtacatgtc gataacaaca tgaccatact gaccacgacc accagactgt ttcgcgtgtt
     1261 taccttcaac atcggtaact ttctggcgga tagtttcacg gtaagcaacc tgcggtttac
     1321 ctacgttcgc ttcaacgttg aattcacgct tcatacggtc aacgatgatg tcgaggtgca
     1381 gttcgcccat acccgcgatg atggtctggt tagattcttc gtcagtccat acacggaaag
     1441 acgggtcttc tttagccaga cggcccagag ccagccccat tttttcctgg tcagctttgg
     1501 ttttcggttc aactgcgatg gagattaccg gctcagggaa ttccatacgt tccagaatga
     1561 tcggcgcatc cgggtcacac agggtgtcac cagtggttac gtctttcaga ccgatagcag
     1621 cagcgatgtc gcccgcgcga acttctttga tctcttcacg tttgttagcg tgcatctgaa
     1681 cgatacgacc gaaacgctca cgtgcagctt tcacggagtt cagtacggta tcaccagagt
     1741 taaccacacc ggagtaaaca cggaagaagg tcaggttacc aacaaacggg tcggtagcga
     1801 ttttgaacgc cagtgcagag aacggctcgt catcacttgc gtgacgttca gccggagtgt
     1861 ctttaccgtc gtccaggata ccgttgatcg caggtacgtc aaccggggat ggcaggtaat
     1921 cgattaccgc atccagcatc gcctgaacac ctttgttctt gaacgcagaa ccacaggtta
     1981 ccaggatgat ttcgttgttc agaacgcgct gacgcagagc acctttaatc tcttcttcag
     2041 tcagttcttc accacccagg tatttttcca tcagctcttc agaagcttca gctgcggatt
     2101 cgatcaggtt ctggtgccat tcgttagcca gttcaaccat gtctgccggg atatcttcgt
     2161 attcgaaggt tacgccctgg tcagcgtcgt tccagttgat agctttcatt ttcaccaggt
     2221 caacaacacc ggtgaaatgt tcttcagcac caatcgccag ctgcagcgga accgggttcg
     2281 cgcccagacg ggttttgatc tggttaacaa ctttcaggaa gttcgcaccc atgcggtcca
     2341 ttttgttaac gaacgcaatg cgcggaactt tatatttgtt tgcctgacgc catacggttt
     2401 cagactgcgg ctgaacacca ccaactgcgc agtaaaccat taccgcacca tcgagaacac
     2461 gcatggaacg ttctacttcg attgtgaagt caacgtgccc cggggtgtcg atgatgttga
     2521 tgcgatgcgg ctcatactgc ttagccatac cagaccagaa tgcagtagtc gcagcggaag
     2581 tgatggtaat accacgttcc tgctcctgct ccatccagtc catggttgca gcgccgtcat
     2641 gaacttcacc gattttatgg tttacaccgg tgtagaacag aatacgttcg gtagtagtgg
     2701 ttttaccggc gtcgatgtgc gcactgatac cgatgttacg gtagcgtgcg atgggtgttg
     2761 tacgagccat ttgtttcctc gtttatcttt taggcgttca atttaagtag cccaaagcgg
     2821 gctgcttact ggaagcgccc gcctggtgac taaaactccg aagggattac caacggtagt
     2881 gtgcgaacgc cttgttggct tcggccatac ggtgaacgtc ttcacgtttc ttaactgcag
     2941 tacctttgtt ttctgcagca tcagaaagtt cgttcgccag gcgcagagcc atggatttat
     3001 caccgcgttt acgagcagct tcaacgatcc aacgcattgc cagagcatta cgacgaaccg
     3061 gacggacttc aactggtacc tgataagtag aaccaccaac gcggcgagac ttaacttcta
     3121 cagtcgggcg cacgttttcg agagctactt cgaatgcttc cagttcagat ttaccagagc
     3181 gctgagccag ggtctccagc gcgctgtata cgatagattc agcagtagat tttttaccat
     3241 ctaccatcag gatatttaca aatttagcca gcagttctga tccgaacttc ggatccggca
     3301 gaattttacg ctgaccaatg acgcgacgac gtggcatgga aatactccgt tgttaattca
     3361 ggattgtcca aaactctacg agtttagttt gacatttaag ttaaaacgtt tggccttact
     3421 taacggagaa ccattaagcc ttaggacgct tcacgccata cttggaacga gcctgcttac
     3481 ggtctttaac gccggagcag tcaagcgcac cacgtacggt gtggtaacga acacccggga
     3541 ggtctttaac acgaccgcca cggatcagga tcacggagtg ctcctgcagg ttgtgacctt
     3601 caccaccgat gtaggaagtc acttcgaaac cgttagtcag acgaacacgg catactttac
     3661 gcagcgcgga gttcggtttt ttaggagtgg tagtatatac acgagtacat acgccacgtt
     3721 tttgcgggca tgcttccagc gcaggcacgt tgcttttcgc aactttgcga gcacgtggtt
     3781 tgcgtaccag ctggttaact gttgccatta aatagctcct ggttttagct tttgcttcgt
     3841 aaacacgtaa taaaacgtcc tcacacaata tgaggacgcc gaattttagg gcgatgccga
     3901 aaaggtgtca agaaatatac aacgatcccg ccatcaccag gccatctggc tggagtgctt
     3961 aaccgtaagt ctgacgaaat cagtatagtc aatgagaatg atatcgttcg aaatttgacc
     4021 agccaaaccg cgggcaataa ggtcttcgtt cagggcatag accttaatgg gggcattacg
     4081 cagactttca aggtagcggt taccgtcaac tgcggcagtt acaccatctt gcaataatag
     4141 cagttcgtcg ccctcactta gcaggcgcag cagcgcagca aaatccgtca gccagggtga
     4201 acgatgtaat gtgtgcagca taaacgcctc aaaacctcaa aataacatcg tagttggcga
     4261 gttcgcggcg taaggcatct gcttcgagcg gcgtggcttc gacaacaaag ggtgtctgcg
     4321 gatctaaccc gcgttcgcgc agtgaagccg cacaaaccca gcactgttca atgtcgtaca
     4381 gacccaacaa tttaaaagtg gcgatgtaat cacgcgccag cactgcatcg ggcttttgtc
     4441 ctggcagcag ctgaaaaacg ccatcagcaa taaagaagac ggccagatcg tcagttaatg
     4501 cggaagttgc cagtaaagca tctaaacctt cccgaccagc agctgtacca tgaggcacag
     4561 tagaaaaaac aaacgcaatt cgtttcatca gaactgtacc accctgtcac aggtcagcga
     4621 ggcttccgcc agcgcgccaa gtccgcttaa ggtaaatccc ggctgaaggt ttgacgaagc
     4681 cagccccagt cttccggcct ccgtttcatc gacgataccg cggcgtaacg ctgccgctac
     4741 gcagatattc agcgccacac catgttgcgc attcaactgt tgccagcagc gcacaaggtc
     4801 aaattcatcg cttgccggtg aggtcaattg gttagcgtta tagacccctt cccgataaaa
     4861 aaagacgctg cttaactcat ggccttctgc aatcagcgcc tgcgcaaact gaaaagcact
     4921 actcgcctgt tgcgtaccgt atgctggccc ggtcaccacg atggcaaaac gcattactta
     4981 tcttgcccct ggaaatcacc gctcttgaac tggcggatgt agagatagac agtgtgtttg
     5041 gagatgttca ggcggtcagc aacctggttg atcgcatctt taatatcgaa gatccctttc
     5101 tcgtagagat tcagcacgat ctgacgattt ttggcgttat tagaaacatt gcgatcggca
     5161 ttcacttctt cgatggtgaa ctccagcgtt tgggtaacca gatcttcaac agaagaggca
     5221 aagttgacgc ttgaaccgac atccggggtt tctggcggca caaaggtgct cataatctgc
     5281 gagaagggaa catcaagatt catattgatg cacagcaaac caattacacg ctgttcgcgg
     5341 ttacgaatcg cgatagtcag ggacttcatt aatacgccgc ttttggcgcg agtaaagtag
     5401 catttagaaa cgctgctatc cgctcccgtc atatcgtgca gcatacgtag cgccaggtcg
     5461 gtaattggcg aaccaatctt ccggcctgta tgttcaccgt tagcaatgcg aatggctgaa
     5521 cattttagat cctgcaaaga gtgcaaaacg atttcacagt gggagccaat aagcatcgct
     5581 aacccgtcca ccaccgcttc gtaggatttc agaatatcaa aatcggtctg gtcgaaagga
     5641 cgttgatcca gtaaatccaa ctcactggtt tcgttggtta aaagcgacct ggacatgaaa
     5701 aaaaacactc cttttcagga gcctgtcgtt aacttttcag ggcaggctca ttaatgatgc
     5761 gggtaactaa attaatacag cggaggttcc gctttccagc actaattata tccggcctgt
     5821 aataaaaaaa ccgccgcctg gtcaggcggc ggttcttaat gcttattttt tagcagaatc
     5881 tgcggctttc gcatcagctt ccggctttgc atcagccttc ggcgctggtt tcacatccag
     5941 cagctctacg tcaaacacca gggtagaatt cggtgggatc cccggaacac ccgctttgcc
     6001 gtaagccagt tctggtggaa taaccagttt gatcttaccg cctttcttga tgttcttcag
     6061 accttctgtc caacccggaa taacaccgtc cagacggaaa gagagcggtt caccacgggt
     6121 gtaagagttg tcgaactctt taccgtcgat cagcgtacct ttgtagttca cgacaacagt
     6181 atcgctgtct ttcggtgctt cgcctttacc ggcttcgact acctgataaa ccagaccagt
     6241 tgaagaggtt ttcacacctt tctctttggc aaatttctcg cggtactctt tacctttcgc
     6301 ttcgttatct gccgcgtctt tttccatctt cgcctgagca gaagacttca cgcgagcttc
     6361 gaatgcttgc agtgtctgtt cgatctcttg gtcggagagt ttgctcttat cagcaaatgc
     6421 atcctgaaca ccagcgatca gctgatcttt atccagtttg atgcccagtt tttcttgttc
     6481 tttaagagag ttttccatgt aacgacccag cgaagcaccc agtgcataag ctgatttctg
     6541 atcgtcattt ttgaacgctg ctttgctgtc agcagttgta gcaggttttg cagcttcagc
     6601 agcaaaagtg atcggtgcat gcagggcaac ggccattgtg gtcgccagca gcgttacttt
     6661 aaacagtgat ttcatccata tctccaggat cggggcatct caccccaggg ttaactatta
     6721 tcagaagggt actataaagc gttgtcgaac aaatctacat acagacacgc cctattatca
     6781 tctattttca gactcttttt gtttaaatta gtttcgatga ccgcgaaatg agtgctgtct
     6841 cgggcagcaa agttaagtag aatccgcggc gaccattcga caaaagaggt gaatcatgca
     6901 ggatttatca ttggaagcac gcctggcaga gctggagagc cgactggctt ttcaggaaat
     6961 caccattgaa gaactgaacg tcacggtgac cgctcatgaa atggagatgg cgaaactgcg
     7021 cgatcatctg cgtctgctga ccgagaagct aaaagccagc cagccgtcga acatcgcgtc
     7081 gcaggctgaa gaaacgccac cgccacatta ttgaggcgta aaaaaagcgg ggattccccg
     7141 cttttttgtc actttttcgg tattagtggc aaccgcaacc gccgttacct ttaccgccac
     7201 agcagccttc gccaccgtgt tcatgaccgt gatcgtggcc atgaccaccg cagcaaccgt
     7261 cgtggtcgtg atcgtggtga tgatcgtgcg caccgtgaac gtggccatga gccagttctt
     7321 cttcagtcgc ttcgcgaatc gccacaactt caacgttgaa tttcaggttc tgaccggcca
     7381 gcatgtggtt accatcaacc acgacgtgat cgtcttcaac cgcagtgatt tcaaccggta
     7441 ccggaccctg gtcggtttca gccaggaaac gcatacctac ctgcagttca tcaacgccca
     7501 taaatacgtc tttaggaacg cgttgcacca ggttttcgtc gtactgaccg taagcgtcgt
     7561 tcgcgccaac agcgacatca aatttgtcgc caacttcgtg accttccagc gccgtttcca
     7621 ggccagagat cagggaaccg tgaccatgca ggtagtccag cggcgcactc accggagact
     7681 catcaaccaa cacaccgtct tctgtacgta cctgataggc caggctgacc accaggtctt
     7741 ttgctacttt catgatatct cctgagcatg ggaagaatag tggcgcagat tgtagcggaa
     7801 ttctgcagcc gtgtactcac tagcttaaaa aaactcggca catatcgcta gtccggatga
     7861 aaaatcccga tcacttgctc atctttgcga acgtgatcgc gggcttcttt gtctgcttct
     7921 cgcatctgat gtccgcactt aacacattca acaatatcaa tattattttc gcgccacatc
     7981 gccattgaat cctgtgcctg acaggctggg cattttgcgc ccgcaataaa acgttttcgg
     8041 attgccatct ttaccctcta ctcaaattca tcccagccgt ccagctggcg tcgttcttgt
     8101 tgcatttcac gctggaaaat ctcttccagt tcgcgtcggg cttccctggc gcgagaaatt
     8161 tgtacggtat cggtatgcat tgggatgagc tctcgcagca tccgcatatc caggcggcga
     8221 aaatgcagtt gcgcccgctg tgcctgatgc ggatgcatgc caagcgtgac cagcgtcttg
     8281 cgccccagct ctaacgcact ggagaatgtt tcacgggaaa actgcgtcac ccctgcctgt
     8341 aataactcat gcgcttccac acgtccgcgc gctcgcgcaa gaatatgcaa atgcggaaag
     8401 tgctgctggc atatttccac cagcttcata gtgtcttccg gctcgttaca ggtaatgacg
     8461 atagactcag cggcctctgc acccgcagaa cgtaaaagat ctacctgcgt ggcgtcgccg
     8521 taataaactt tgtagccata tttgcgcatc aggttaacgg cactgatatc ccgctccagt
     8581 acagtaatgc gcattttatt tgccatcagc aaacgaccaa tcacctgacc aaaacgccca
     8641 aagcccacga caatgacctg cggtttatca tcgttgaccc atggtttttc gtcttcttct
     8701 tccggtccgt taaactggcg ggatagccat ttatctacca gcttcatcag cagcggcgtg
     8761 gtcatcatgg aaagcgtcac cgtcaccagc aacagcgcca tctggtcgcc ctggaataag
     8821 cgttgtgaag aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg
     8881 ccagcaaact gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc
     8941 acgagaattt tcaccgccac cagcacaacc acacttatca ctacccacaa cagatgggta
     9001 taaagcaccc cgaggttgag cgacatgccg acagagataa agaacaaacc aagcagcaag
     9061 cctttgaagg gatcgatagc cgtttccagt tcatgacgat attcactttc cgccagtagc
     9121 acacccgcaa taaacgtacc gagcgccatc gacaacccca gcgcatccat aaacaatgcg
     9181 gaacccaaca ccagcagcag cgtcgcggcg gtgaacactt cccgcacacc agaagctgca
     9241 ataaaacgga ataccggacg cagtaaatag cgcccgccaa tcagcatgcc gacaaacgcc
     9301 agcaccttca tgccgatctt catccagtcg aaatgttcgt ctgccgaacc cgccaacaac
     9361 ggcactaacg ccagtgccgg gatcaccgcc agatcctgaa acagcagtac cgagaaacct
     9421 aactggccgg attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca
     9481 gttgaagaca tcgcaaggcc aatgccaccg accaccgccg cctgccatga gaaatgggtc
     9541 agcatcaata atcctgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca
     9601 aaaatcgaac gccgcagttg ccaaagcttg gagggattca actcaaggcc gatgataaac
     9661 atcaggaata ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac
     9721 cccagccccc acgggccaat cgcaatccct gccagcaaat atcccaacac agcgccaata
     9781 cccagccgcg atgccaacgg caccgcagcc accgccgcga agagaaacag cactcctgcg
     9841 agtaaaaaat cggaaccttc catcagcggc ctcctggaga cagcggattt gccagccagt
     9901 caccgtaggc tctggcgtgg ctcgccagct cctgtgcgct ttgccgtctc gcccagtaaa
     9961 taatgatggg acttaaccaa tgcatccggc acatgcccgc cgccagttca aaggggcgca
    10021 gaacatcgct catcgggtag cgattcaagg cgtcatagcg gtaagcactt tccggctcgc
    10081 cagtggtaat tacgctacgc cagtactttc ccgccagttg gtttcccccc ggcccgctgg
    10141 caaaaccacg acttaatacc cggtccagcc actctttcag tagcgccggg cagctatagg
    10201 tataaagagg atgctgaaag acaatcacct cgtgctcgcg cagtaatgcc tgctcacggg
    10261 ggatatcaat aaaaaaatcc ggatagtgcg cataaaggtc gtgcacggta acattgctga
    10321 gctgcgtggc cggtttaagc agtacccggt ttgccaccga gtcctgagat tccggatggg
    10381 catacagcag caaaactttc gctggctgag acatcatccc cctcccggta tggtttttgt
    10441 gtatagtcgc tgttttgggc taccattgcg cccggtgcgg cagctcgccc atacattaca
    10501 ttatcataat gataagttaa catagtctga acatacggcg ccttatgatt gttttttcct
    10561 cgttacaaat tcgtcgcggc gtgcgcgtcc tgctggataa tgccaccgcc accatcaacc
    10621 ctgggcagaa agtcggcctg gtgggtaaaa acggctgtgg taaatctacc ctgctggcat
    10681 tgctgaaaaa tgaaatcagc gccgacggcg gcagctacac ctttccggga agctggcaac
    10741 tggcgtgggt gaatcaggaa acgccggcgt taccgcaagc ggcgctggaa tatgtcattg
    10801 acggcgaccg tgaatatcgt caactggaag cgcagctaca cgacgccaac gaacgtaacg
    10861 acgggcacgc catcgccact attcatggca agctggatgc tattgacgca tggagtattc
    10921 gctcccgtgc cgccagcctg ctgcacggcc tcggtttcag caatgaacaa ctggagcgcc
    10981 cggtaagtga tttctctggt ggctggcgta tgcgtcttaa ccttgcccag gcgctgattt
    11041 gccgttcaga cttgctgctg ctcgacgaac cgactaacca cctcgatctc gatgccgtta
    11101 tctggctgga aaaatggctg aagagctatc agggcacgct gatcctgatc tctcacgacc
    11161 gcgacttcct cgatccgatc gttgataaaa ttattcatat cgaacaacaa agcatgttcg
    11221 agtacaccgg caactacagt tcgtttgaag ttcagcgcgc cacccgtctg gcgcagcagc
    11281 aagcgatgta cgaaagccag caggaacgcg tggcgcatct gcaaagttat atcgaccgtt
    11341 tccgtgccaa agccaccaaa gcgaagcagg cccagagccg cattaagatg ctcgagcgta
    11401 tggagctgat tgccccggcg cacgttgaca acccgtttcg ttttagcttc cgcgcgccgg
    11461 aaagcctgcc taatccgtta ttaaagatgg aaaaagtcag cgcaggctat ggtgatcgca
    11521 ttattctcga ctcgattaaa ctgaatctgg tccccggctc gcgcattggt ctgctaggcc
    11581 gcaacggcgc gggtaaatcg acattaatca aactgttagc cggtgaactt gcgccagtca
    11641 gcggtgaaat tggtctggcg aaagggatca agctcggcta cttcgcccag catcaactgg
    11701 aatacctgcg cgccgacgaa tcgcctattc aacatctggc acgtttagcg ccgcaggagc
    11761 tggagcaaaa actgcgtgac tacctcggcg gctttggttt ccagggcgat aaagtaaccg
    11821 aagaaacgcg ccgcttttcc ggtggggaaa aagcccgcct ggtgctggca ttaattgtct
    11881 ggcagcgtcc gaatctgctg ctgctcgacg aaccgactaa ccaccttgac ctcgacatgc
    11941 gtcaggcact gactgaagca ttaatcgagt tcgaaggcgc gctggttgtc gtttcgcacg
    12001 accgtcattt gctgcgttcc accactgacg atctctacct ggttcacgat cgtaaagtcg
    12061 aaccgttcga cggcgatctg gaagattatc aacagtggtt gagcgacgta caaaagcagg
    12121 aaaaccagac cgacgaagcg ccaaaagaga acgcaaacag cgcccaggca cgtaaagatc
    12181 agaagcgtcg ggaagcggag ctgcgtgcgc aaacccaacc gctgcgtaaa gagatcgccc
    12241 gtctggaaaa agagatggaa aagctgaacg cacagctggc gcaggcggaa gagaaactcg
    12301 gcgacagcga actgtacgat caaagccgta aagcggagtt gaccgcctgc ctgcaacagc
    12361 aagccagcgc caaatccggc ctggaagagt gcgaaatggc gtggctggaa gcccaggagc
    12421 agcttgagca gatgctgctg gaaggccaaa gcaactgatg gcgcagataa cgacgaccga
    12481 tgccaatgaa ttcagcagca gtgctgaatt cacccctatg cgcggcttta gcaattgtca
    12541 tctacaaacc atgctgccgc gtctgtttcg tcgcaaggtg aaattcaccc cgcactggca
    12601 gcgactggag ttgcccgacg gcgattttgt cgatctcgcg tggagtgaag atcctgcaca
    12661 ggcgcggcat aaaccgcgtt tagtcgtgtt tcacgggctc gaaggcagtc tcaatagccc
    12721 ttacgcccac ggtctggttg aggcggcgca aaagcgcggc tggctgggcg tggtgatgca
    12781 ttttcgcgga tgcagcggtg aaccaaaccg tatgcaccgc atttaccatt cgggtgaaac
    12841 cgaagacgcc agctggtttt tacgctggct gcagcgcgaa tttggacatg cgccaacggc
    12901 tgccgtcggc tattcgctcg gcggtaatat gctcgcctgt ttgctggcaa aagaaggtaa
    12961 taacctcccg attgatgcag cggtaattgt ctccgcgcca tttatgctgg aagcctgtag
    13021 ttatcatatg gaaaagggct tttcccgcgt ttatcagcgt tacttgctga acctgttaaa
    13081 agccaatgcc gcgcgcaagc tggcagccta ccccggaaca ctgccgatta atctcgcgca
    13141 gttaaaatcg gtacgtcgca tccgtgaatt tgacgatcta atcaccgcca gaattcacgg
    13201 ctacgctgac gctatcgact attatcgtca gtgtagcgcc atgccgatgc tcaatcggat
    13261 cgccaaaccg acgctgatta ttcacgccaa agacgatccg tttatggatc atcaggtgat
    13321 cccgaaaccg gaaagtctcc ccccgcaggt ggagtatcaa ctgactgaac atggcggtca
    13381 tgttggcttt attggcggta cattacttca tccgcaaatg tggctggagt cacgcattcc
    13441 tgactggtta acaacgtatc tggaggcgaa atcatgttga ttccgtggca agacctctcc
    13501 cccgaaacgc tggaaaattt aattgaaagc tttgtgttac gtgaaggcac cgattatggt
    13561 gaacatgagc gtacacttga acagaaagtc gccgacgtca aacgccagct acagtgcgga
    13621 gaagcggtgc tggtatggtc ggagctgcac gaaacggtca atatcatgcc gcgcagtcag
    13681 tttcgcgaat aacctgcaac cgtcggcgga cgagataata atgatagcta agtcatggag
    13741 ttactatgtc tgccaaacat ccggtcattg cggtaacagg atccagcggc gcggggacca
    13801 ccaccaccag cctcgcgttt cgtaaaatat tcgcgcagtt aaatctgcat gcagccgagg
    13861 tggaaggcga cagttttcac cgttacaccc gcccggaaat ggacatggcg atccgcaaag
    13921 cacgcgacgc cgggcggcat atcagctact tcggccccga agcgaacgac ttcggcctgc
    13981 tggaacaaac cttcattgaa tatggtcaga gcggcaaagg gaaatctcgc aaatatctgc
    14041 atacctacga tgaagccgta ccgtggaatc aggtaccggg gacattcacc ccctggcaac
    14101 ctttaccgga acccactgat gtactgtttt atgaaggttt acacggcggc gtagtcacgc
    14161 cacagcataa cgttgcgcag catgtggact tactggtcgg cgtggtgcct atcgttaacc
    14221 ttgagtggat tcaaaaactg atccgcgaca ccagcgagcg cgggcactca cgagaagcag
    14281 tgatggactc ggtagtgcgt tcaatggaag actatatcaa ctacatcaca ccgcagtttt
    14341 cccgcaccca tcttaacttc cagcgcgttc ccaccgtcga cacttcaaac ccgttcgcgg
    14401 caaaaggtat cccgtcgctg gatgaaagct ttgtggtgat ccattttcgt aatctggaag
    14461 ggatcgattt cccctggctg ctggcgatgt tgcaaggctc attcatttcc cacatcaata
    14521 cgttagtggt accgggcggc aaaatgggtc tggcaatgga attaattatg ctgccgctgg
    14581 tgcaacgatt gatggaagga aagaaaatcg agtaactttg ctattacgcc ggataaaata
    14641 ctatccggct tcacaatggg atagttaaat cacgcggcaa ccacttcata cgagtgagta
    14701 atattcaccg ctttttccag catcaacgcc actgaacaat atttctcggc agagagatca
    14761 accgcacgtg caaccgctgc gtctttcagg tcgcgaccgg taacgataaa atgcagatta
    14821 atgtgcgtaa acaggcgtgg tgcctcttcg cggcgttcag aggtcaattt tacttcacaa
    14881 tcgaccacat cctgacgccc tttttgcagg atagaaacca catcgatggc actgcaaccc
    14941 cccgccgcca tcaacaccat ttccatcgga cttggtgctt tatcgcctga gttgccgtcc
    15001 attaaaatct ggtgaccaga ggcggattcg cccagaaaag ttaacccttc gacccacttc
    15061 actcgcgctt gcatttttgc tactccactg cgtcaatttt cctgacagag tacgcgtact
    15121 aaccaaatcg cgcaacggaa ggcgacctgg gtcatgctga agcgagacac caggagacac
    15181 aaagcgaaag ctatgctaaa acagtcagga tgctacagta atacattgat gtactgcatg
    15241 tatgcaaagg acgtcacatt accgtgcagt acagttgata gccccttccc aggtagcggg
    15301 aagcatattt cggcaatcca gagacagcgg cgttatctgg ctctggagaa agcttataac
    15361 agaggataac cgcgcatggt gcttggcaaa ccgcaaacag acccgactct cgaatggttc
    15421 ttgtctcatt gccacattca taagtaccca tccaagagca cgcttattca ccagggtgaa
    15481 aaagcggaaa cgctgtacta catcgttaaa ggctctgtgg cagtgctgat caaagacgaa
    15541 gagggtaaag aaatgatcct ctcctatctg aatcagggtg attttattgg cgaactgggc
    15601 ctgtttgaag agggccagga acgtagcgca tgggtacgtg cgaaaaccgc ctgtgaagtg
    15661 gctgaaattt cgtacaaaaa atttcgccaa ttgattcagg taaacccgga cattctgatg
    15721 cgtctgtctg cacagatggc gcgtcgtctg caagtcactt cagagaaagt gggcaacctg
    15781 gcgttcctcg acgtgacggg ccgcattgca cagactctgc tgaatctggc aaaacaacca
    15841 gacgctatga ctcacccgga cggtatgcaa atcaaaatta cccgtcagga aattggtcag
    15901 attgtcggct gttctcgtga aaccgtggga cgcattctga agatgctgga agatcagaac
    15961 ctgatctccg cacacggtaa aaccatcgtc gtttacggca ctcgttaatc ccgtcggagt
    16021 ggcgcgttac ctggtagcgc gccattttgt ttccccctat gtggcgcagg ctgatttatc
    16081 accccgatat caactatgca cttcgacaaa cgctggtgct atgtttgccc gtggccgttg
    16141 ggttaatgct tggcgaatta cgattcggcc tgctcttctc cctcgttcct gcctgttgca
    16201 atattgcggg ccttgatacg cctcataaac gttttttcaa acgcttaatc attggtgcgt
    16261 cgctgtttgc cacctgtagc ttgctgacac aggtgctact ggcaaaagat gttcccctgc
    16321 cctttttgct gaccggatta acgctggtac ttggcgtcac tgctgagctg gggccattgc
    16381 acgcaaaatt gcttcccgca tcgctgctcg ccgccatttt taccctcagt ctggcaggat
    16441 acatgccggt ctgggaaccg ttgctcatct atgcgttggg cactctctgg tacggattgt
    16501 ttaactggtt ttggttctgg atctggcgcg aacaaccgct acgcgagtca ctaagtctgc
    16561 tgtaccgtga actggcagat tactgtgaag ccaaatacag cctgcttacc cagcacaccg
    16621 accctgaaaa agcgctgccg ccgctgctgg tgcgccagca aaaagcggtc gatttaatta
    16681 cccagtgcta tcagcaaatg catatgcttt ccgcgcaaaa taataccgat tacaagcgga
    16741 tgctgcgtat tttccaggag gcgctggatt tgcaggaaca tatttcggtc agtttgcatc
    16801 agccggaaga ggtgcaaaag ctggtcgagc gtagccatgc ggaagaagtt atccgctgga
    16861 atgcgcaaac cgtcgccgct cgcctgcgcg tgctggctga tgacattctt taccatcgcc
    16921 tgccaacgcg ttttacgatg gaaaagcaaa ttggcgcact ggaaaaaatc gcccgccaac
    16981 acccggataa tccggttggg caattctgct actggcattt cagccgcatt gctcgtgtgc
    17041 tgcgcaccca aaaaccgctc tatgcccgtg acttactggc cgataaacag cggcgaatgc
    17101 cattacttcc ggcgctgaaa agttatctgt cactaaagtc tccggcgcta cgcaatgccg
    17161 gacgactcag tgtgatgtta agcgttgcca gcctgatggg caccgcgcta catctgccga
    17221 agtcgtactg gatcctgatg acggtattgc tggtgacgca aaatggctat ggcgcaaccc
    17281 gtcttaggat tgtgaatcgc tccgtgggaa ccgttgtcgg gttaatcatt gcgggcgtgg
    17341 cgctgcactt taaaattccc gaaggttaca ccctgacctt gatgctgatt accaccctcg
    17401 ccagctacct gatattgcgc aaaaactacg gctgggcgac ggtcggcttt accattaccg
    17461 cagtgtatac cctgcaactg ttgtggctga acggcgagca atacatcctt ccacgtctta
    17521 tcgataccat tattggttgt ttaattgctt tcggcggtac tgtctggctg tggccgcagt
    17581 ggcagagcgg gttattgcgt aagaacgccc atgacgcttt agaagcctat caggacgcga
    17641 ttcgcttgat tctgagcgag gacccgcaac ctacgccact ggcctggcag cgaatgcggg
    17701 taaatcaggc gcataacact ctgtataact cattgaatca ggcgatgcag gaaccggcat
    17761 tcaacagcca ttatctggca gatatgaaac tgtgggtaac gcacagccag tttattgttg
    17821 agcatattaa tgccatgacc acgctggcac gggaacaccg ggcattgcca actgaactgg
    17881 cgcaagaata tttacagtct tgtgaaatcg ccattcagcg ttgtcagcag cgactggagt
    17941 atgacgaacc gggtagttct ggcgatgcca atatcatgga tgcgccggag atgcagccgc
    18001 acgaaggcgc ggcaggtacg ctggagcagc atttacaacg ggttattggt catctgaaca
    18061 ccatgcacac catttcgtcg atggcatggc ggcagcgacc gcatcacggg atttggctga
    18121 gtcgcaagtt gcgggattcg aaggcgtaat acaggctaaa ccatcggata aggcgttcgc
    18181 gccgcatccg acatcttttg cctgatgcgt cgtatttacg ccccaatcac cttcgccacc
    18241 gcgtgggcga aacgatgcat ccctttatcg atatccgcat cttccaccac cagcgacggc
    18301 gcaaaacgca tcacatcagg cccggcattc agcaccatta cgccttcctc tgcaccggca
    18361 tacaggaaat cacgcgcccg acctttgtac tgtggtttca gctccgcgcc aattaacagc
    18421 cccataccgc gaatatcgct aaacacatcg tactgctgat cgatcttctg caaatggtca
    18481 acaaaacgct ggcgtttcgc ctgaatgcct tccagcactt caggggtatt gatgatatca
    18541 aacgccgcgc ccgctactgc acaggccaga ggattaccgc cgtaggtgga accgtgagaa
    18601 ccaggatgaa atgcagaagc aatttccgcc gtggtcagca tggcgctaat cgggaagccg
    18661 ccgcctaacg ctttcgcaga ggtcagaata tccggcgtca caccgtagtg catgtaagca
    18721 aacaaatcgc cagtccgccc catcccgcac tgcacttcat caaacaccaa taatgcctga
    18781 tgttgatcgc acagctcgcg caagccctgc aaaaactctg gcgtcgctgc cgtcacaccg
    18841 ccctcgccct ggatcggctc aaccaccacc gcacaggtat ggtcatccat caccgctttc
    18901 actgcgtgga gatcgttaaa aggcacgtgg atgatgtctg ccggttttgg cccaaagccg
    18961 tcggaatatt ttggctgccc acccaccgaa acggtaaaca gcgagcgacc atgaaaagcg
    19021 ttatggaagg caataatttt ggttttgaac gggctatgac gcacacaggc gtaatggcgt
    19081 gccagtttaa aggcggtttc gttagcttcc gtgccggagt tcataaacac cacgcgttcg
    19141 gcaaaagttg cctcaatcag tttacggcca agacgcagcg ccggctcatt ggtgaaaacg
    19201 ttactaatat gccacagagt ttcgccctgg gtttttaacg cgttcaccag cgcaggatgg
    19261 caatggccca acgccgtcac tgcaatgcca cccgcgaaat cgacatactc cttgccctgt
    19321 tgatcccaga ttcggctgcc ctgacctttt accggaataa actctgccgg agcataaatc
    19381 ggcagaatca cttcatcgaa agtcgcgcgt gtaattgctg tttgttcaat tgccatctca
    19441 tgatcaccct gttacgcata aataaatatg aaattataat cacaaaatat gcataaaaaa
    19501 taactgaatg gcaatcagaa atcagcgatg cagaaaatta gccagcagtt gatgtccttg
    19561 ttcgctgaga atactttctg gatggaattg cacaccttcc agatcccact ggcgatggcg
    19621 aatccccata atctcgcggg tttcgctcca ggccgtcact tcaaagcact ccggtaatga
    19681 gtcaggttcc accaccagcg aatggtagcg tgtcacggtg agtggatttg ccagcccctt
    19741 aaatacgccc acaccattat gtgtaatcgg cgaggttttg ccgtgcatga cctttgcggc
    19801 gcgcacaact ttgccgccaa atgcctgcgc cattgcctga tgaccgaggc agacgccaag
    19861 aatcggcaag cgtccggcat agtgacgaat aacgtcaagg gagatcccgg cttcatctgg
    19921 cgtacagggg ccgggtgaga tgacaatttt ttgtggttta agggcgtcga tatccgccaa
    19981 cgtcaacgca tcgttgcgct taaccaacac atccgccccc agttcacaaa agtactggta
    20041 gaggttccag gtaaaagaat cgtagttatc aataagcagg atcatggcgg ctccggtacg
    20101 aaagaaccgc gctattttac tcagattccc cggcttcgct taccactttg ctaaatatca
    20161 tctgcagcgc ggtgagatcc cccattgccc cattctgatt tgcctgattc caggcctctt
    20221 tttcaatacc ctgccagctc agttgatatc cggcatgaat cgccagttgc tcgaagaaga
    20281 tccgctgtgc caaaccactt cccacccgga agggatgcag cacgttgatt tcacaatagt
    20341 aatgcgccag ccgctcgacg aacttcgctt tctccaggcc aaccagataa ccttcttcct
    20401 ccagatcctg catcagggca ttgccctctt tttcgatata agcaaagtgg cagaacggcg
    20461 tatcaccctg ataaatatca acttcacgca gttgccccgc ccagtcgaaa atatcctgat
    20521 acagctggcg atggatagtg cgcaaatgcg gtaaaccgcg caccagcgga ccaagctcaa
    20581 tggtcgcagc acgtagcgcc gtcatttcgt aagcggcctg ttccagccgc tgttgctggc
    20641 ggatgttcag ccggttacgc atgatatcaa ggccaggata aagatacgga tcgcgccctt
    20701 cgccgaattt atcgctcata gtgcctcctc agctcttcaa ggcgcgctaa agcctctgcg
    20761 gcagtaagag tgactaaagg catctcgacg ccttcaagac ggcgactggc ctggaaatta
    20821 cgattacgct gaagctccca gagacgggac ttttgcttat cggtgagttt cttcacttga
    20881 tgcctccctg aacgtgtcca tttgccacaa gtataagcgg caaatccggg ttacgccggg
    20941 agagcagaag cgcgggcaag attgcccgcg cgagaaatca ttacggcagg actttagcgg
    21001 agaggataac taccggtttt gacggcacat tctggtacgg accaacgtca tgagtcggca
    21061 cctgggaaat cttatcggca acgtccatgc ctttcaccac tttaccaaat accgcgtaac
    21121 cgaaatcacg ctgaccatgg tcaaggaagg cgttatcggc aacgttgata aagaactggc
    21181 tggtggcgct gtctttgtca gcggtacgcg ccatcgcgat ggtgccacgc gtgttgcgca
    21241 ggccgttatc ggcttcattt ttgattggcg ggtttggttt tttctgctgc atctgctcgg
    21301 tgaaaccgcc gccctgaatc ataaagccag gaatgacgcg gtgaaaggta gtgttgttat
    21361 aaaaaccgct gttcacataa tcgacaaagt tttgcacaga cactggcgct ttttgtttat
    21421 ccagctccag ttcgatatta ccagctgagg ttgtcaacaa tacgtgcggg tcccctttcg
    21481 ctgccattgc tgcgggagaa agagcagaaa gagcgaaaac agcagccatc gccgccaggg
    21541 tcgatttgaa catgagattt ccttaacaaa cagagcagaa aaaaagcaag tgaagagatt
    21601 ctaaatagcc ttcaagaaca aggccatccc tttacctaat tttacgtatc tgaaatatat
    21661 gtaacctgac gatcactttt tattctgtga tcaaaatcac ctcttaaaat gcaatttagc
    21721 aaccgattgc aataaaacat ttaatcagat cacaaaatca cctaaaatcg cccgctcgcg
    21781 gcatagcccg ctatgccatt ttatttttga acgcaggcca atcatgacta acagcaatcg
    21841 catcaagctc acatggatta gctttctctc ctacgcactg actggtgcgt tggttattgt
    21901 caccgggatg gtgatgggaa atatcgccga ttatttcaat ctgcctgttt ccagtatgag
    21961 taacaccttc accttcctca acgccggcat tttaatctct atcttcctca acgcctggct
    22021 gatggaaatc gtcccgttga aaacgcagtt acgttttggc tttctcctga tggtgctggc
    22081 ggttgccggt ttgatgttca gccacagcct ggcgctgttc tcggcggcga tgttcattct
    22141 cggagtggtc agcggcatca ccatgtcgat tggtacattc ctgataacac aaatgtatga
    22201 agggcgtcag cgcggttccc gcctgttatt taccgactcc ttcttcagta tggccgggat
    22261 gattttccca atgatcgccg cgtttctact ggcgcgcagc attgagtggt actgggttta
    22321 tgcctgcatc gggttggtgt atgtcgctat ttttattctg accttcggct gtgagttccc
    22381 ggcgctgggc aaacatgcgc caaaaacgga tgctccggta gagaaagaaa agtgggggat
    22441 cggcgtgctg tttctctcca ttgcggcact gtgctacatc ctcggtcagt taggttttat
    22501 ctcctgggtg ccggagtatg ccaaaggcct gggcatgagc ctgaacgacg cgggcacgct
    22561 ggtgagtaac ttctggatgt catatatggt cggcatgtgg gcgttcagct ttattcttcg
    22621 cttctttgat ttgcaacgta ttctgaccgt actggctggt ctggctgcga ttctgatgta
    22681 cgtctttaac accggaacac cagcacatat ggcgtggtca attctcgctc tgggcttctt
    22741 ctccagcgcg atctatacca ccatcatcac cctgggttca cagcaaacca aagtaccgtc
    22801 gccaaaactg gttaactttg tcctgacctg cgggaccatc ggtactatgt tgacctttgt
    22861 ggttaccggc ccgattgttg aacatagtgg tccgcaggcg gcgctgctga cggcaaacgg
    22921 tctgtacgct gtcgtctttg tgatgtgctt cctgttaggt ttcgtcagcc gtcaccgtca
    22981 gcataacacc ctgacctctc attaattgtt catgccgggc ggcactatcg tcgtccggcc
    23041 ttttcctctc ttactctgct acgtacatct atttctataa atccgttcaa tttgtctgtt
    23101 ttttgcacaa acatgaaatg tcagacaatt ccgtgactta agaaaattta tacaaatcag
    23161 caatataccc cttaaggggt atataaaggt gaatttgatt tacatcaata agcggggttg
    23221 ctgaatcgtt aaggtaggcg gtaatagaaa agaaatcgag gcaaaaatga gcaaagtcag
    23281 actcgcaatt atcggtaacg gtatggtcgg ccatcgcttt atcgaagatc ttcttgataa
    23341 atctgatgcg gccaactttg atattaccgt tttctgtgaa gaaccgcgca tcgcttatga
    23401 ccgcgtacac ctctcgtctt acttctctca ccacaccgcc gaagagctgt cgctggtgcg
    23461 cgaaggcttc tacgagaaac acggcatcaa agttctggtc ggcgaacgcg ctatcaccat
    23521 caaccgtcag gagaaggtga ttcactccag cgccggacgt accgtttttt atgacaagct
    23581 gatcatggcg accggttcct acccgtggat cccgccaatt aaaggttctg atactcagga
    23641 ctgctttgtc tatcgcacca ttgaagacct caacgccatt gaatcctgcg ctcgtcgcag
    23701 caagcgcggt gccgttgttg gtggcggcct gttaggtctg gaagctgctg gcgcgctgaa
    23761 aaacttaggc attgaaaccc acgttatcga atttgcccct atgctgatgg cggaacagct
    23821 tgatcagatg ggcggcgagc agctgcgtcg caaaatcgaa agtatgggcg tgcgcgttca
    23881 caccagcaaa aacactcttg agattgtgca ggaaggtgtc gaagcgcgta aaaccatgcg
    23941 ttttgccgac ggcagcgaac tggaagtcga ctttatcgtc ttctctaccg gtatccgtcc
    24001 gcgcgataag ctggcaaccc agtgtggtct ggacgttgct ccgcgtgggg gtatcgtcat
    24061 aaatgattcc tgccagactt ccgatccgga tatctacgcc atcggtgaat gcgcaagctg
    24121 gaacaaccgt gtatttggtc tggtagcacc aggctacaaa atggcgcagg tcgccgttga
    24181 ccatattctt ggtagcgaaa acgcctttga aggtgcagac cttagcgcca agctgaaact
    24241 gctgggcgta gacgtaggcg gtattggtga tgcgcacggt cgcacgcctg gcgcacgtag
    24301 ctacgtttac ctcgacgaaa gcaaagagat ctacaaacgc ctgattgtca gcgaagacaa
    24361 caaaaccctg ctcggtgcgg tactggtggg cgataccagc gactacggca acctgctgca
    24421 actggtgctg aacgctatcg agctgccgga aaacccggat tccctgatcc tgccagcgca
    24481 ctcgggtagc agtaagccgt ctatcggtgt tgataaactg ccggacagcg cgcaaatctg
    24541 ctcctgcttc gacgtcacca aaggcgacct gattgctgct atcaacaaag gctgccacac
    24601 cgttgcggcg ctgaaagccg aaaccaaagc cggtacgggc tgcggcggtt gtattccgct
    24661 ggtcactcag gtactgaacg cggaactggc gaaacagggt atcgaagtta acaacaacct
    24721 gtgtgaacac tttgcttatt cgcgtcagga actgttccat ttgatccgcg ttgaaggcat
    24781 taaaaccttc gaagaactgc tggcgaaaca cggcaaaggt tacggttgtg aagtttgtaa
    24841 accgaccgtc ggttcgctgc tggcctcctg ctggaacgaa tacattctga agccggaaca
    24901 tactccgctg caggattcta acgacaactt cctcgcgaac atccagaaag acggcaccta
    24961 ctcggtgatc ccgcgttctc cgggcggtga aatcaccccg gaagggctga tggcagtggg
    25021 tcgtatcgcg cgtgaattta atctctacac caaaatcacc ggctcccagc gcctggcgat
    25081 gtttggcgca cagaaagacg atctgccgga gatctggcgt cagctgattg aagccggctt
    25141 cgaaaccggt catgcctatg cgaaagcact gcgtatggcg aaaacctgcg tgggtagcac
    25201 ctggtgccgc tacggcgttg gcgacagcgt cggcctcggc gtggaactgg aaaaccgcta
    25261 caaaggcatc cgtacgccgc acaaaatgaa gttcggtgtc tccggctgta cccgtgaatg
    25321 ttcagaagcc cagggtaaag acgtgggtat tatcgccacc gaaaaaggct ggaacctgta
    25381 tgtttgcggt aacggcggca tgaaaccgcg tcatgcggat ctgctggcgg cggatatcga
    25441 tcgcgaaacg ctgatcaaat atctcgaccg cttcatgatg ttctacatcc gtactgccga
    25501 caaactgacg cgtaccgcac cgtggttaga aaaccttgaa ggcggcatcg attacctgaa
    25561 agcggtgatc attgacgaca aactggggct gaacgcacat ctggaagaag agatggcgcg
    25621 cctgcgtgaa gcggtagtgt gtgagtggac tgaaacggtc aatacgccgt ctgcgcagac
    25681 tcgcttcaaa cacttcatca acagcgacaa gcgtgacccg aacgtacaga tggtgccaga
    25741 gcgcgaacag caccgtccgg caacgccgta tgaacgtatc ccggtaactc tggtggagga
    25801 caacgcatga gccagtggaa agacatctgc aaaatcgatg acatcctgcc tgaaaccggc
    25861 gtctgcgcgc tgttaggtga cgaacaagta gcaattttcc gcccgtatca cagcgatcag
    25921 gtgtttgcga tcagcaacat cgacccgttc ttcgagtcca gcgtgctgtc acgcgggttg
    25981 attgcggaac accagggcga gctgtgggtc gccagcccgc tgaaaaaaca gcgttttcgc
    26041 ttaagcgacg gcttgtgcat ggaagacgaa cagttttccg tcaagcatta cgacgcgcga
    26101 gtgaaagacg gcgtggtaca gctgcgcggt taatatttta acgggaggcg caatgcctcc
    26161 cctttttgca tggtcctgta ataaccttcg gtatattgca ggacattttt taaa
//
LOCUS       DAPHCV010000074        26007 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.074, whole genome shotgun sequence.
ACCESSION   DAPHCV010000074 DAPHCV010000000
VERSION     DAPHCV010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.074"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(29..4489)
                     /gene="mukB"
                     /locus_tag="RH033_003007"
     CDS             complement(29..4489)
                     /gene="mukB"
                     /locus_tag="RH033_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HDV2992159.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPYQAS"
     gene            complement(4489..5193)
                     /gene="mukE"
                     /locus_tag="RH033_003008"
     CDS             complement(4489..5193)
                     /gene="mukE"
                     /locus_tag="RH033_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HDV2992160.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5174..6496)
                     /gene="mukF"
                     /locus_tag="RH033_003009"
     CDS             complement(5174..6496)
                     /gene="mukF"
                     /locus_tag="RH033_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HDV2992161.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMVLR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6493..7278)
                     /gene="cmoM"
                     /locus_tag="RH033_003010"
     CDS             complement(6493..7278)
                     /gene="cmoM"
                     /locus_tag="RH033_003010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HDV2992162.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQILGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7414..8193
                     /gene="elyC"
                     /locus_tag="RH033_003011"
     CDS             7414..8193
                     /gene="elyC"
                     /locus_tag="RH033_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HDV2992163.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPIADRLLRPIENTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            complement(8170..9063)
                     /locus_tag="RH033_003012"
     CDS             complement(8170..9063)
                     /locus_tag="RH033_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HDV2992164.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEDHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9217..9963)
                     /gene="kdsB"
                     /locus_tag="RH033_003013"
     CDS             complement(9217..9963)
                     /gene="kdsB"
                     /locus_tag="RH033_003013"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HDV2992165.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHENVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKGLETVGDNFLRHLGIYGYRAGFIRRYVNWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(9960..10142)
                     /gene="ycaR"
                     /locus_tag="RH033_003014"
     CDS             complement(9960..10142)
                     /gene="ycaR"
                     /locus_tag="RH033_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HDV2992166.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10194..11426)
                     /locus_tag="RH033_003015"
     CDS             complement(10194..11426)
                     /locus_tag="RH033_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HDV2992167.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLGDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGRC
                     PQGPFTDCRTCWEIDPVA"
     gene            complement(11463..12449)
                     /gene="lpxK"
                     /locus_tag="RH033_003016"
     CDS             complement(11463..12449)
                     /gene="lpxK"
                     /locus_tag="RH033_003016"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HDV2992168.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTGAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     NAGQTLVMTEKDAVKCRAFAEGNWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            complement(12446..14194)
                     /gene="msbA"
                     /locus_tag="RH033_003017"
     CDS             complement(12446..14194)
                     /gene="msbA"
                     /locus_tag="RH033_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HDV2992169.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLMWMPLVVIGLMILRGITSYISSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14231..16495)
                     /locus_tag="RH033_003018"
     CDS             complement(14231..16495)
                     /locus_tag="RH033_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HDV2992170.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLASLTVFACVLVFIPF
                     KTVRYIALTLLFFVWGILAAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVFLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALAMWGMLKLSGRQWSGWDVWICCLVAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPKWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     FAIPLVTFITVPLILAAMVVHLSGPFILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLTFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCLPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGSNDKGNNHSCVVK
                     IDDGTNSILLTGDIEAPAEQKMLSRYWQQMQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQITVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(16702..16986)
                     /gene="ihfB"
                     /locus_tag="RH033_003019"
     CDS             complement(16702..16986)
                     /gene="ihfB"
                     /locus_tag="RH033_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HDV2992171.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17146..18819)
                     /gene="rpsA"
                     /locus_tag="RH033_003020"
     CDS             complement(17146..18819)
                     /gene="rpsA"
                     /locus_tag="RH033_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDV2992172.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18930..19613)
                     /gene="cmk"
                     /locus_tag="RH033_003021"
     CDS             complement(18930..19613)
                     /gene="cmk"
                     /locus_tag="RH033_003021"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDV2992173.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19786..20550)
                     /gene="ycaL"
                     /locus_tag="RH033_003022"
     CDS             complement(19786..20550)
                     /gene="ycaL"
                     /locus_tag="RH033_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HDV2992174.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGINTDLAISSGLNAYKAATL
                     SDADAKAIANQGCVEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            complement(20720..22003)
                     /gene="aroA"
                     /locus_tag="RH033_003023"
     CDS             complement(20720..22003)
                     /gene="aroA"
                     /locus_tag="RH033_003023"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDV2992175.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALAALGVSYTLSADRTRCEIIGNGGPLHAKSALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTAVTILDPKCTAKTFPDYFEQLARISQSG"
     gene            complement(22074..23162)
                     /gene="serC"
                     /locus_tag="RH033_003024"
     CDS             complement(22074..23162)
                     /gene="serC"
                     /locus_tag="RH033_003024"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HDV2992176.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMSEWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNDSMFNTPPTFAWYLSGLVFKWLKANGGVAAMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(23361..24053)
                     /locus_tag="RH033_003025"
     CDS             complement(23361..24053)
                     /locus_tag="RH033_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDV2992177.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMNAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            24183..25943
                     /locus_tag="RH033_003026"
     CDS             24183..25943
                     /locus_tag="RH033_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HDV2992178.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTIYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWSFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         6331 a         7085 c         6301 g         6290 t
ORIGIN      
        1 aaaaggcggc attgctgccg ccttcatttt aactcgcttg gtaaggcgct tcgtcacttc
       61 ctggaagcgt ttcagggagt tgcggcgcaa atcctcgcag gccgacgacg tgaacgtgtt
      121 cggtattctg gaagacttta cgcaccagtt tataggtggt gcctttttcc ggactgatat
      181 tttccggcgc tgcgatgatg agctgcattt gcaaacgttc acacaattca aacagcgtgg
      241 cgatagaacg agcatccagt cgcgctgctt catcgaggaa cagcaggcgg caaggagaga
      301 tatctttacc gcgcaggcgg cgagattcat cttcccagct ttgtaccacc atcaccagaa
      361 tcgacatacc ggtaccaatc gcctcaccgg tcgacagagc gccagattct gcgcgcagcc
      421 agccatcaga accacggtta acctcaactt ccatttccag atagttgcgg taatccagca
      481 gttcttcgcc aatggtctgc ggcgtgcgct gccccatatc aatctgcgga ttaagacgct
      541 gatacaattt cgccagcgct tccgagaagg tcagacggtt gctgttaaac agatcctgat
      601 gctgctcgtg ctgttcagag agcacatcca gcagcatggc gtgcgtttca cgcacgttca
      661 cattcagacg tacgctgttt acctgaccga acgatacgtt ctgcaaaccc tggttgagca
      721 tacggatacg gttctgttcg cgctgaatgg ttttgcgaat aatgttcgcc acgctgcggg
      781 aactgatcgc cagtttctgt tcacgggagg ttaactcttc ggtcagacgg ctaagttcga
      841 tctccatctg ttcgatagct tccaccggat catcggtacg aataatatcc tgacgaatac
      901 gttcacgcag atgctgataa accgccacga agaactgaat tttacgttcc ggacgttttg
      961 gatcttccga catccgcagc acgtcgcgca gatgttcgtt atccgccacc gccagacgca
     1021 gcgcacctaa cgccttatcc gacatggaac gcaaatcatc agcggagaga taagccagct
     1081 cacgacggtg taagcggcgt tcaacgccgt tatctttcac catgcgcatg actgcacacc
     1141 agcccgcttt cgcggtcact acctgctcgc gcatctcaaa gtaatcacgc tccagcttgc
     1201 gcagtttgcg ggtcaggttg tccatctccg cttcgcagaa ggtaagcgct ttttccagtt
     1261 gattgcggcg tgaacggtta ttgctcagtt gcgcgtgcaa ctcgtcacgg cgaatacgcg
     1321 cccgctcttc tgccccgcta tcagcacgca cgccgatatc ctgcaattca cgttgcagat
     1381 cgttgagtag ctcttttttg gtgtcgtaag aacttttcag cgaagccagc acctggttgt
     1441 actgactcaa ctgcgctgcg tgtccgcgca acgcttcacg cgcgcgggta cgctccgctt
     1501 ccgcctgttc cagacgttca cgcagttttt cgttgagatc gctgttaccg ctcagcattt
     1561 ctgccgagtc agagtagcta aagtgcgcac gacgctgcac cacttccgtc agggcaaacg
     1621 cctgctgacg ggcatcgcgc tgcatctgct gagagtacgc gtaatcttct tttaactgtt
     1681 cgaactgttc cgggtcgctc tgcaataccg aaacgatcgg ttccagtttc gccagttgat
     1741 tgccaaactg ctgaacaaaa cgcgcggctt cctgggcttc atccagacgt tcgcggattt
     1801 catcgacgcg atccgccagg ctgtcatcag ccaacaggtt gagacgcggc agaatgcggt
     1861 tcagcgccgt aacgccctct ttcgcctgct caaattgaat acgctgctgc tggttatcat
     1921 tttcatgatt actcagcgcc cgctccagtt cgacgcgacg gctgttcagt tgacggattt
     1981 cggcttccgg atcagactca aacgccaccg ccagatgact acctataaag cggctgaacg
     2041 cctgatgcag acgctgggtt ttctgtacat caaaagagag cgtggcgaag cgttcggaaa
     2101 gcacttcacg ctcggcatgg aggctttcaa tacggctttc acgcgcagca cgaccaaaca
     2161 gcggaacttc cggaaaacgt gagtaacgcc actgacgatc ggcgattttc accactaccg
     2221 ctttttccag ctcatcaacg ctgaacacgc tgtcatcgaa tgactgcgga tccccttcga
     2281 tcagatagag atcttccggg caatcggtca agccttccag gtgttcggtt acctgtgaca
     2341 gatctggcac cacgatggcg tggcgtgacg ggccatacag tgccgagaag tacggcgcat
     2401 cttccagact cacgtcgtca taaatttcgg acagcagcac gccgccaaaa cgctccgcca
     2461 gcgcattcaa acgctggtct tcagaaccac ctggctggct taaacgttcg atctcttcat
     2521 cgacggcgtt tttgcgcgcg cctacttcat cgcgttcaac aatggcttcg cgctcacgct
     2581 ccagcaactg ttgcagatat tcagtgacgt cctggctgga ggtgaactct tcgccgcact
     2641 gttcgctcaa ctggttgaga ctgttttgcg ctgccagcca aaccggtgca cgctgcatca
     2701 aactctgaat gcgagactgc agctgttcct gttcctggcg cagtgccatc cgctcttctc
     2761 gggcgttaga gacgctatcg gaaagagagg caatgcgtgc ttccagctcc tgatgcagag
     2821 cttccagttc atcaatatca aaattcttgc cctgacgttt gcagaaatct gccagcagac
     2881 gctcagcttc ttgctgctca cgcagacgct gttccagttc gcttaagcgc atccgcaacg
     2941 gctgaacctg ctctgccagg tgacgctgat cgaccccttc gcgcaatagt tcgcgagcga
     3001 catcccacgc ctcgttacgc gccagcgggc cgttgattgc caccaccagc tgataagcct
     3061 gctcaaactg gctgtgcgcg gtttgcgcca tgctcatttt ctgctcaaga gagagcattt
     3121 tttcggtcgc ttccagctct ttcgcctgga aggtttccag ccattcggca gcgctgtcgg
     3181 cggttaagtc cggcagatgg cacagttctt tggcacgatt aagcgcagca atcgcctggt
     3241 tatactggat cgcgcgcgtt tgctggacgt ccagcgcctg ctggtagtca gcaagctggc
     3301 ttttcagctc gtccacttcc agttcggcag cttccgcacg agcctcgttc tcttcctggc
     3361 gttcgatggc ttctgccacc acttcgtttt gctcttccag acggatctgc agctcatcga
     3421 gatccgcttc gtagcgttcg atcttctcct gctgacgcag tgcggtttgc accaggttca
     3481 ggtgatcgct ggccgcctga taatccgctt ccagatcgcc ttcggcaccg ttgtgctcag
     3541 ccagctctcg cgccatgtcg acgtgtttgt actgctcagc cgccagttgc tgacgcgaag
     3601 tatgtagctc gcgacgaaac tccagggcct tatcgagatg aacacggcgc tcgttggcgt
     3661 gacgcatgta gtccgccgcc acgtagttgg tggcttcgct gatcagatgc ttaaacaggt
     3721 cacggtctga ctgggtgaca cgaatcgctt ccagcgtcat gcggttttca cgcagcgccg
     3781 cttccatgtc ctggaacgct ttccgcaccc cactgttttc cggcaacagg tagtcgcgca
     3841 gagaacgagt aatggcgctg gagatcccgc catacagcga ggcttcgatc agacgataga
     3901 atttactacg gtcagacgct gagcgcagac gacgcgcgat gatgcccaga tcgaacatca
     3961 gcgagtggta atcggtaatg gagttgaact gtttaaactg cacgccttcc atcgcctcga
     4021 gcttgtcttt cagctcgtta agcggcagca cgcgcgcctg gcgttcgttc agagtttcgg
     4081 tcaccagttg tgtcggctgc accgacatcg gcaggccctg aatggcaaat ggtttgatat
     4141 ccactttacg atcgcgtccg gcaacctgtt gcagacgcac accgaccact acacgctggt
     4201 ggcgcgagtt aatggtgtcg agcatcgaat aacagacacc cgctttcagc ttaccgtgca
     4261 gacctttatc gcgcgaaccg ctggtggccc cggcttccgt agtgttacgg aaatgcagca
     4321 gggtcaggtc ggggataagc gccgtaacga acgctgccat ggtggtggat ttacccgccc
     4381 cgttaccgcc agaaagcgtc gtgaccagct cgtcaaggtc aaaagttcgg gcaaaaaagc
     4441 cgttccagtt aatcagcgtc agtgagcgaa atttaccgcg ttcaatcatt attcttcctc
     4501 tccgctatct ggctgactct cttcggtttc atcgttgagt tgcagatgat tttcaatcgg
     4561 cattgcttcg ccatcacgaa tcaggcgacg ctgtgcttca cggggatcgt cgccagcacg
     4621 cacatcggct ccgaagcgga acaccgattc ggtaatgcgg aacttgctgc tgtcgtggcc
     4681 cataaaccac accataccca aacgacgcag acggttgagc gaagaacgta ctttctcctg
     4741 caacttctgc cggtcaacgt ctgaaccggt tgaacggttg ttcaccagtt tcagcagttt
     4801 cgcttcatcg gccagggtga gcagttcgtc gtacagttcc tgctgggtga aaatcccctc
     4861 attcgccagc cgttccgggc tgagatagag ataacagagg attttcccga ccatcatatc
     4921 cagttccgac aagacggaac gagggatcag cgtggtggaa cgtgggcgta gatagaagaa
     4981 cccttccggt gcacgaataa gctcaacgtt ataacgcgcg taaaactctt ccagatattc
     5041 ctgaaaatcc atcaggaatg catgattatc cagttcgtcg aggccaatat gacggcctga
     5101 acgtaaggcg ctatccagcg ccggaaataa cggattcgcc agcgcctgcg ccagcttaac
     5161 cggcatcact tgttcaatat ttgtcgatga catgcgcctg taccttggct ccgtaatcat
     5221 taatcggctg ccatttcgct ggcagtccgg tgaaatctgc ttgcgctacg ccaagacgta
     5281 ccgcctgatc aataacaatg cgcgcaacgt caaagtgacg tgcacgcgga tactgtgaca
     5341 gatattcgcg taccaccaga ccaagatcca acggcacttg tctggttttg tacacggcaa
     5401 gttgttcttc gatgatcgcc gccagctgtt cgcggatctc gttaaactct tcgtactcca
     5461 gatcctcagg aagttcccca gtcacttctt catcgcgcag taccatctct tcgtcacgca
     5521 tatccagcag acgatcggca ttggcataag ttagcgccca cggctcatca aaataggttt
     5581 gtaccgactg acgtaaccgc tgagcaaaga cgcggttttt atccatatcg atcgcagtac
     5641 ggataaattt gtgtacgtgg cggtcgtagc caatccacaa gtctatggat tgctggcccc
     5701 aactgataat acggtcgagt ttgctctgca gatcgaacac cagacgatcg acaaaatgca
     5761 ggtcgtcatg ggtcatcgtc gcatcctgaa tgcgcaacag attagcctgc aatttatcgc
     5821 ctgccgcttc cagcgtatcc tgcaattcac gcagcgttcc ggaagtttct gaaagcaaca
     5881 attcacagct ggaaatcgcc gcccgccagt ctttgttcaa caactgggcg atatcatcct
     5941 tcacctgctg ctgctgttcg tccatcagac gttgcgtcag gtcgatactg tcgaaaattt
     6001 ctgctaccga atatttcagt ggcgcataaa cattacggtg ccagtgaaat tcatcaccgc
     6061 cctcttcggc ggcatctgcc gcgcgtttga gctcacccgc cacaatcgac aactgcatag
     6121 aaaggcgcag cgtagaaaac tcgcgctgac gaatgtaata gtcagtgatg ccgatgccga
     6181 gcggcgtcag acggtaaatt gcgttccctt ccgcctgctc gctggtaaag cggttcagca
     6241 gacgttgacg caccatgtcg ttgatcgcgt tgttggcgcg cacgccgatg gtttcgctgg
     6301 tttgctcaaa cgcatcactc acatggcgga atgcatccac cagctcgcct tcactcatct
     6361 caccatccag acgctcgccg ttcagcgtgg caaccgccag cagaaaagag agtcggtcta
     6421 ccggcagcga gatggagaag tcattttttc tggcccaggc aaccagttcg gggactgtct
     6481 gggaaaattc actcatactt tatccttgct ctgcggtttg cgcgcggtga catgaatata
     6541 acgccccagg gtaatatacg gttcctgacg gcaataacgc gtttctaatt caagtaatgc
     6601 ttcatagcag tcgcgctgct gatgcttctc gcgcagataa tcatgaaaca cgcgaacgcc
     6661 tgtcttaccc agaatttgcc aaccagcttc ttccagccac agataaacct gcgccgggtc
     6721 gcgtggataa tctggcgaaa gcgtccgttt tttctttttc ggcattcccg cctgcacgta
     6781 atcaaaattc ccggcgacca tgttatgcat caacaaacca tgcgcattgt agaacattaa
     6841 cgacaacacg ccgcctggac gcaacactga ccacagggtc tgtaatacgc tgcggggatc
     6901 ggccacccac tcgagcaccg catggaacaa tatcagatca acgggcgttt ccaaatgcga
     6961 agcaacatcc tgagcggcgc aatgtataaa ttgcatgttg tcgctcacac cttttgcttc
     7021 tgccgcctgt tttgcgcgat cgatcatctg cgcagaaaga tcgcataaaa tgacctgatg
     7081 cccacgctcg gccattttga ttgcggtctg cccttctcca ccgccagcat ccagcacacg
     7141 cagtttttgc gggcccattt ccgccagcac gcgatcgaga tcctgccaca gaatagcctg
     7201 tcgaagctgc cctttggtgg tgccgtaaat gttacgggaa aacttttccg caatatcatc
     7261 aaaattgcga tcctgcattg ctttctccac cgcttggcta agacgttttc tgataaagac
     7321 gctattttgt cacagccacg gcgagaatga acccgtctgg tgtaatatcg ctggcgatcc
     7381 cttgctatat ggtcaaaaaa ggaaccagaa aggatgcttt ttacactgaa aaaagtgatt
     7441 ggcaatatgt tgctgccact tccgctgatg ttgttgatta ttggcgctgg cctggcgctg
     7501 ctgtggttta gccgttttca aaaaaccggg aagattttta ttagcattgg gtggctggcg
     7561 ctcttgttat tgagtttgca accgatagcc gaccgcctgt tgcgtcccat cgaaaacact
     7621 tatccgacat ggaataattc gcaaaaagtg gactatatcg tggtgctcgg aggtggttat
     7681 acctggaacc cgcagtgggc gccgagctct aatttaatca ataacagtct gccacgcctg
     7741 aatgaaggta ttcgcttatg gcgcgaaaat ccgggatcaa aactgatctt tacgggaggc
     7801 gtagctaaaa ccaatacggt gagcacagcc gaagtaggtg ccagagttgc gcaatcgctg
     7861 ggcgtgccgc gcgagcaaat tatcaccctg gatttgccaa aagataccga agaagaagct
     7921 gcagcagtga aacaggcgat tggtgacgca cctttcctgc tggtgacctc cgcatcacat
     7981 ctgccgcggg cgatgatctt ttttcagcag gaagggctaa atccgctgcc agccccggcg
     8041 aatcagttgg ccatcgactc gccgcttaat ccgtgggaac gggcaatccc ttctccggtt
     8101 tggttgatgc atagcgatcg tgttggctat gaaacgcttg gccgcatctg gcagtggcta
     8161 aaaggttcgt caggcgagcc acggcaagag tgattttgat gccagatcga agttgcgccg
     8221 actaaatcgt ccggtattaa ccagttgcgc aacttcatcc cataacacat acaaccaacg
     8281 ccgccagatg aacgactccg ccactggcgc gcgttgcagg taactccaga gcaaatcttc
     8341 tgccagagaa ttatccatta gtcggaacag ttcgtattct cgcggtgccc aaagcattag
     8401 tcccggcccg accatcgcca gtaactgatc gctgcgcgaa tctttcagca tgctgcgtaa
     8461 acagaagtta ccgtgaatca gcacacaatt gtcgttaaag ccttcaaata atgccgggag
     8521 acattcgcga gtacgaaaca ggatccgctt atcctgcatc gttagaccgg tgttattgaa
     8581 ctgattgagc gtggtccata gcacttcaac atgttgccgg taccatgagg gccagaaatt
     8641 ttcctgagta ttgtcgaccg cgccgacgca accgcgactg tcctgacggt gccaggccag
     8701 taaggcttca acgatttgat ctttgagttg ttcccagcgt tctggtgttc gggctggtgc
     8761 ctccaccgaa acaccacgca tccgctccag tagcaggaca tcggggccgg ggtggtcttc
     8821 atgcgtcatc acaccataaa tagtcggcat acggacagtc ccacttcttg ccagcatggt
     8881 ggttttccat gccagttgtc gggcttttcc gggcgtacta aagcttcttg ccattaacgg
     8941 cattgggttt ccctggctgt catacaaagc ccacaacgcc gtatccgctt tttcattgac
     9001 gcactcaata cgactgagtt tttcgcccag taaatggctt aattcggcac gcagctgttc
     9061 cattcagatt accctcataa aagctactgc tttcataatg agcgccaata taaaagatgt
     9121 cacctgaatg agatcaattg ttgaaaagaa aaattgagga aaatcgtaac ggtacgacac
     9181 tcctcccaaa attggctgga gtgtcgtgaa gtgaaattag cgcatttcag cgcgaacgcg
     9241 ctcgagatct tcaggggtat ccacacctgt gccaggaact tcctgggcga cagcaacatg
     9301 gattttttcg ccgtaccaca gaacacgaag ctgctctaac atttcgatgt gttctaacgg
     9361 acttggctgc cagttgacgt aacgacggat aaagcctgca cggtagccat aaataccaag
     9421 atgacgcagg aagttatcgc caacggtttc aaggcctttt gcaaaacgat cacgatccca
     9481 aggaatggtg gcgcgagaga agtacagtgc atacccttca gcgtcgagga ccactttcac
     9541 cgcattcggg ttaaacgctt cctccgcatt gtggattggc accgccagag tcgccatacc
     9601 cacctgacgc tgggcgagat tatcagcaac ctgacgaatg attgtcgcag ggatcatcgg
     9661 ttcatccccc tgcacattaa cgatcaccgt gtcgtcgctg aatgcgcatt tttcgacaac
     9721 ttccgccaga cgttctgttc ctgactgatg atcggcgcgc gtcatacata cttcaccgcc
     9781 agcggcttca acagcgcggg caacattctc atgatcggtt gccacgatga tgcgctcggc
     9841 acctgattcg cgcgcgcgtt caagaacatg aacaatcatg ggtttgccgt taatatcaac
     9901 caatggttta ccgggcagac gcgtggacgc gtagcgcgcg ggaataatga ccacaaaact
     9961 catgatttac tctcatcagc agtcagcacg cgggcttccg tttccagcaa cactggaatg
    10021 ccatcgcgca gcgggaaagc caggttgtcc agtttgcaaa taagttcttg tttttcctgg
    10081 ttataccaaa gttttccgtt gcaaaccggg caggcaatga tttcaagcag acgatgatcc
    10141 atagttcctc cagatggatc gggttatgaa tgcataaatc ttatcataat catttatgcg
    10201 acggggtcta tttcccagca ggtgcggcaa tcagtaaagg ggccttgcgg gcagcgtcct
    10261 aatgtcaccc gcgttgcctg ctgccagtta gcgaaatcag taatcgcctg acgtaaccct
    10321 ttttgcagcg ttgtcgttgg tttaatgcct tcttgtaacc acagagagat aacttcaagg
    10381 atgcctgtct ggcgatgcat tttggcatcc attcgcccaa ctaattgccc acgatgtaat
    10441 aacggcagaa caaaatagcc atactggcgt ttcggcgctg gggtatagca ctccagccgg
    10501 tagctaaaat caaaaagctg ctctgcgcgt ttgcgatccc agacaacagg atcaaaaggc
    10561 gaaagtaccg cgctgtgcgt tgcagtgagc tttccggcca gcgcacgctc gagtagcggc
    10621 agcaaatcag catgcagcca aagattgccc aatttttcaa catgcacagc aatgatttgc
    10681 tgctgttcag ccctcgcttc gcgccaagcc gccagtgccg gacgtttcag ccgatagtaa
    10741 tctgccagcc actgttcgcg gaatatgccc aggctacgcg cactgttatc cagcatgatg
    10801 atttctgctt ctgtttgcga aacgagatcg cgctcatcat cccagtcagg catgacacga
    10861 tgggttaaat cataaacgcg ctggaagttg cgccgttcaa tcaccatcac ctttccggca
    10921 gtaaataaac cttccagatg ccgtttatgc ggcttccatt cccaccagcc gcttgcacct
    10981 ttacgaggat gctcaaaatc ggctgaacgt accggcccct tatcatgaat atgctgaatt
    11041 aactgtgcaa tttccgcctc atgttcctgc atccaggcgt ctttgtattt ccagcccatt
    11101 ttttcaggtg ccagcatgcg gtggcgaata agacgaaagt cgctacgcgg cataaagcag
    11161 gcttcatgcg cccagtattc cattaattcg ccacgcgcca gagactcatc cagccactgg
    11221 gcaggataat ttcccagacg actgaaaagc accagatatg gactacgggc aacaatatta
    11281 atggtatcga tttgcagcaa ggacatgcgg gagatcgttg ccggaatatc ccccaacgac
    11341 gctcgacggc ggggtttgtt taacaggcct tgcgcggcga ggtgaagatt acgcgcatca
    11401 gcaagagaga ggtgcggcag cgacattcat gactccatca atcgaacgct gccgcggcgt
    11461 aactagttgc cagaagccag cgaggttagt tgcgcaagca gtttcgctgg ttcatcacct
    11521 gaaagctgtg cgtcaacagg caaataccac caatttcctt cggcaaaggc ccggcatttc
    11581 accgcatctt tttcagtcat taccagcgtt tgcccggcgt ttaccaacgc gctgacatcc
    11641 gcatggttca aagactgatg atcggccagc ggtacacatt tttccggttg tacgccgcac
    11701 atcttcagcg tggcaaaaaa gcgcggcgga tgtccaatcc ccgccatcgc cactacatgt
    11761 tcaagctgag caacgtcaca acgcgtaccg gtacgtaaat tcaccgcctg acccggcagc
    11821 agatgcatgg ggatttcacc gctgcggggg acaccgccgt tgacgattac cgcatcaacc
    11881 gactttaagc gccccgctcg ctcacgcatt ggccccgccg gcaaccacca gccattgcca
    11941 aagcgacgca caccatcaat aacgacaatt tccacatcac gcgccagacg ataatgctgt
    12001 aaaccatcgt cggttacgat gatctgcaca tcagggtgtt gcgccagaat ggcttttacc
    12061 gcgtcagaac gcacgggaga aaccgcaaca ggcgcgccag tgcgttgata aatcaacaca
    12121 ggttcatcac ccgcctgtgc tgtggtggta tctgccgaca ataacagcgg ataagattca
    12181 gccttaccac catatccccg cgatacaacc cccacgcgaa taccgcgctg ttgcagctgt
    12241 tccaccagcc agacaacgac cggggttttt ccgttgccgc ctgcggtgag attaccaacc
    12301 acgacaaccg gtacgggggc acgccaggcg cgcttcagtt ttagtttata gcaaagacgg
    12361 atcgcgccac tcaccaggcc atacaaccag gagagtggca gcaatagccg ccataaaggg
    12421 gattcaccag accagatttt ttcgatcatt ggccaaactg cattttgtga agttgcgcgt
    12481 aaacgccacg gtgctcaagc aaatcgttat gcgtaccgcg ttccacaatg acaccatcct
    12541 cgacaaccac gatttcgtct gccttttcaa tggtagacaa gcggtgggca atcaccagag
    12601 aggtacggtt tttctgcaac tcatccagtg ccgcctgaat cgcacgttcg gattcggtat
    12661 ccaacgccga ggtagcttcg tccagaatca gtatcgggct atcacgcaac aaggctcgag
    12721 cgatagcaat acgctgacgc tgaccgccag agagcagcac gccgttttca ccaatcactg
    12781 tatcgagacc gttatccatc ttattgatga agtccatggc gtaggccata cgcgccgctt
    12841 cttcaatttg ctcacggctg tactgttcag tccgtgcgta agcaatgttg ttagcaaccg
    12901 tatcgttaaa cagatggaca ttctgcgaca ccagagcaac ctggttacgt aacgacgcca
    12961 gggtatactc gcgcagatcg tgaccatcca tcaggatttc gccttcatca atatcgtaaa
    13021 aacgcgtgat caggctggcg atggttgatt tacctgaacc agagcgtcca accagagcaa
    13081 ccgtcttccc tgccggaatt ttcaggttga tgttacgcaa tgcgggcaca tcacgtcccg
    13141 gataagtaaa ggtgacattg cggaattcca cgtcgccagt cgcacgctcg atcacgcgct
    13201 taccttcgtc tttctcctgc tcactgtcca gaatggtaaa cagcgtctga caagccgcca
    13261 taccgcgctg gaactgggcg ttaacgttgg tcagcgattt cagtggacgc atcagtgcaa
    13321 tcattgaaga gaaaacaacg gtaatcgtac cggcagtcag gctatccatg acacttggga
    13381 agcttgccgc atacagaaca aacgccagcg ccaaagaggc gatcagctga atgatcggat
    13441 cagagatgga agaggctgaa accattttca tcccctgaag acgcattcgg ttgctgactt
    13501 tatcgaagcg tttcgtttcc acttcctgac caccgaaaat caatacttct ttatggcctt
    13561 tcagcatttg ttcggcgctg gtggtcacct gccccatggt gttctgcatg tttttactga
    13621 tgttgcgaaa acgcttcgat acaacgcgaa tcgcaatcga aacaatcggt gccagcacaa
    13681 tcaaaatgat cgacagttgc caactgtaat agaacatcat gatgaacagg ccgatgatcg
    13741 acgcaccttc acgcacaaca gtaatcagtg cgccggaaga agaagaagcg acctgttcgg
    13801 aatcgtaggt gatacgtgac aacagcgtac ccgttgactg tttgtcaaag aatgaaaccg
    13861 gcattcccat catgtgacca aacaggcggc gacgcatagt cattaccacc tttcctgata
    13921 cccaggagat acagtagctg gagatatagc tggtgatacc acgtaaaatc atcagcccga
    13981 tcaccaccag cggcatccac atcagcacgg agcgatctgt tttaccaaag ccatcatcaa
    14041 gaagtggctt aaggagcgat aacatgaagg tatcgctggc tgcgttgagg attaacgcta
    14101 cgcccgccac gatcagaccc gctttaaaag gcgcaatggt tggccacagt cggcggaatg
    14161 tctgccacgt agagagatct ttgtcgttat gcattcaaaa aaccagcatt tgttgaaata
    14221 gccgcatatt ctacccgtta tccactggca cgccaaacca ctgatgatac caacgaggta
    14281 aaatttgctc tctgaggctg ctaatccgcc agccttgcgc tgaaaaattg accgttattt
    14341 gaccctgatg tggagtatca agccacttat aaccctgttg ttgatagcga tgtttaactt
    14401 tgcttgaggg cagtcgccat gcgttatagc gcgatgccga tgcgagtgcc acttttccat
    14461 tcactcgctg aattaatggc aacgatgatg aggtattact gccatggtga ggtacctgaa
    14521 gtaatgttgc ctgcatttgc tgccagtaac ggcttagcat cttttgttca gcgggggctt
    14581 caatatcacc ggttagaaga atgctattcg tcccgtcatc aatcttaacc acacaggaat
    14641 ggttatttcc tttatcgttg ctaccttgta aaggccagtg cgcgctgaaa cgcaatcctt
    14701 gccattgcca cgcttcgcca cgcacacagg gctgatgatg ttcccagttt aacggacttc
    14761 ggatccataa catcggccat gtgtgcaata ttgagtccag ccctccccgg tgatccagat
    14821 gttcatgact caaaataacg ccttccggtt caagattatg ccagtggagc caggggataa
    14881 tcagttgttg cccactatcc ccttcgggcc aggccagtcc cgtgtcatag agaatcgctt
    14941 tgccgtttcg cgcaatgacc attgccagcc cttgcccgac atcaagcatg tacacttgcc
    15001 actcgtcagg tcgaggtttt tgccacagcg gcaagcacat cagcaagcct acagccacac
    15061 acattgctgg caacgttcgc caggcgttta atcgccatac caccagtaag aaccatgggg
    15121 aaaatgttag ccattgccaa cgttcagcaa tgttgatcca cccttccggc aaactcttta
    15181 acccccagaa aagtaaagcc aaagaccggt cggcaagaaa ccataaccct tgttccagaa
    15241 taaacggccc gcttaaatgc acaaccatcg cggcgagaat caacggaacc gtgataaatg
    15301 tcaccaaggg aattgcaaaa agatttgcaa taaacgaggt cagactaatg ccatgaaata
    15361 tgacaatttg cacgggcata agcaggagtg tgattcccag ttgcagatgg atgagggaaa
    15421 caaccgcgcg caataccggc ggcagctgcc acttcggaca gggaaaccac tgataccaaa
    15481 aaatcagtgc cgcgaccgca gcggcagaga gccataaact ttgcgagaga atggcaacag
    15541 gatccatcag caaaattgcc accagacaac atatccatac atcccagcca ctccattgtc
    15601 gcccacttaa cttaagcatt ccccacatgg caagcgccac cacggtacgc aatgcaggag
    15661 gttgcatccc agtcagccag gcataaaaag cagcacagca gattccgcca attaatggca
    15721 tttgccagtg gatccagcgc ccaggcagaa aaacttgccc accgcgaatg agtccggcag
    15781 ccagcaacgc cgcaaaagcg atatgcaatc ccgatatcgc cattaaatgc gccgttccag
    15841 tatcgcgcat gatatttttg atttctttgg gaacggataa ccgttccccc atacctaaac
    15901 caagaataac cgcgttccac atatagggtt gcagcgttgt ttgtagtgac gccagatact
    15961 gtgcacgcag gctacaattc ggttcaatga cacttgcctg cagaaagcgg ccggtgagcg
    16021 gctgatgctg ggcaatggca taacgctggc tatcaaagcc gccatcatta agttgaccat
    16081 gaactgcacg aactttgagt ttcattgacc acagttgtcc agcacaaacc gcttgcggaa
    16141 gatattcgcc atacagcacg aggcctggcg cagggaatat acgtcgacct tgtagatgag
    16201 taatttgacc gtaatgagtg gtcataccgt cagtagcagt gatctcaaca attgcatcct
    16261 gcgtcgcgcc agttaaggtt tctcctgccc acaaaatttg ctttgctgct aatatgcccc
    16321 aaacgaaaaa cagcaacgtc agcgcgatat aacggacagt tttaaaaggg ataaatacca
    16381 gtacgcaggc gaagacggtc agagacgcaa gggttaatgt cccaggcaat tggggcaaaa
    16441 tcagcaacgg aaaaattccg caaattatgc atacgccgac tgtcgttatt ttcatgcacc
    16501 cctccctgtt tatggaaagt gtgcaacttt gttgagcggt tgtctggtgg gagttttgcg
    16561 ttccccgagg cacgttcaga agaatttaac gagagggtga aaactgttca aaactctgcg
    16621 aggctgcttc cagttccaga ttagagaaat gaaaaaagca cccgacaggt gcttttctcg
    16681 cgttcaagtt tgagtaaaaa cttaaccgta aatattggcg cgatcgcgca gttctttacc
    16741 tggtttaaag tgaggaacgt attttccttc cagttctact ttatcgccag tcttcggatt
    16801 acgtccggta cgtggtgcgc ggtagtgcaa agagaaactg ccgaaaccgc ggatttcaat
    16861 acgctcgccc tgcgcaagag tcgaggccat atgctccagc atctctttta ctgcatcttc
    16921 aaccgtcttg gcgggaatgt gcgattgctg ggtggcaagt ctttctatca attctgactt
    16981 ggtcatgatt cctccggttc cttaaaggca aatttagcgg ctgctgttgc tgcattgatt
    17041 aagggcggcc gtagccgccc ttagtgcttg attacaggac gaaacctgca atctgtcaag
    17101 taaactcaac aaacttcgga ataaaaatcc cgaagagtca gagaattact cgcctttagc
    17161 tgctttgaaa gcttcagcca ttgcgttgtt ggagaagttt gcatcttcct gtttgttaac
    17221 agttgcgatt gcatctttct cgtcagcttc gtctttcgca cgaacagaca ggctgattgc
    17281 gcggttttta cgatcaacgc cggtgaattt agcttcaact tcgtcgccaa cgctcagaac
    17341 cagggtagcg tcttcaacgc ggtcacggga tgcttcagaa gcacgcaggt aaccttcaac
    17401 gccgtcagcc agttctacgg ttgcgccttt agcgtcaact gcagttactt taccggttac
    17461 gatagcgcct ttcttgttca gagcaaccca gttgttgaac ggatcttctg cgagctgttt
    17521 aacgcccagg gagatacgtt cacgttctgc gtcaacctgc agaacaactg cagcgatttc
    17581 gtcgcctttt ttgtattcac gaactgcttc ttcgcctgca acgttccagg agatgtcaga
    17641 caggtgaacc aggccgtcga tgccgccgtc caggccgatg aagataccga agtcagtgat
    17701 agacttgatt ttaccttcaa cacggtcgcc cttgttgtgg gtttccgcga actgctgcca
    17761 cgggttagct ttgcactgtt tcagacccag ggagatacga cgacgttctt cgtcgatatc
    17821 cagaaccata acttccacta catcgccaac gttaacaact ttggacgggt ggatgttttt
    17881 gttggtccaa tccatttcgg aaacgtgtac caggccttca acgccttctt cgatttcaac
    17941 gaagcagccg tagtcggtca ggttggtcac gcgaccagtc agtttggtac cttccggata
    18001 acgtttagcg atagctaccc acggatcttc gcccagctgt ttcaggccca gggatacacg
    18061 ggtacgttcg cggtcgaact tcagcacttt aacagtgatt tcgtcgccca cgttgacgat
    18121 ttcgctcgga tgcttaacgc gtttccaggc catatcagtg atgtgcagca ggccgtcaac
    18181 gccgcccaga tcaacgaatg caccgtagtc agtgaggttc ttaacgatac ctttaacttc
    18241 catgccttcc tgcaggtttt ccagcagttg atcgcgctca gcgctgtttt cggattcgat
    18301 aacggcacga cgagaaacaa caacgttgtt gcgcttctga tccagcttga ttactttgaa
    18361 ttcaagctct ttgccttcca ggtgcagagt gtcacgcacc ggacgaacgt ctaccagaga
    18421 acctggcagg aacgcacgaa taccgttcag ctcaacagtg aagccgccct taactttgcc
    18481 gttgataaca ccggtaacag tttcagcatc ttcgtaagct ttttccagcg tgatccaggc
    18541 ttcgtgacgt ttagctttct cacgggacag cagagtttca ccgaagccgt cttctactgc
    18601 gtccagagca acgtcaactt cgtcacctac ctggatttcc agctcgccct gggcgttttt
    18661 gaactgctca gccgggatgg cggactcaga tttcagacca gcgtcaacca gtactacgtc
    18721 tttgtcgata gcaacaacaa cgccacgaac gatagaaccc gggcgggttt cgatttcttt
    18781 taaggactct tcaaagagtt gagcaaaaga ttcagtcatg tttaatcttc aggtttatat
    18841 ttaacgtcca cctggctcca tgccggatgg ggttgtttaa catacccgct gtcattccat
    18901 tgcaacgggg gtactgcaaa ttcggtcgct tatgcgagag ccaatttctg gcgcgcgtat
    18961 tgtagcgctt tttcaatcac ttgctcaatg cttaaggtgg tggaatccaa cactaaagca
    19021 tcggctgccg gaaccagtgg cgctaccgct cggttacgat cgcggtcgtc gcgttctttg
    19081 atctcggcca aaaggcgctc aaagttaaca ctaaagccct tctcctgcaa ctgtagcatg
    19141 cggcgatgcg cacgttcttc cgaggaggcg tcaaggaaaa ttttcactgg cgcatcaggg
    19201 aataccaccg ttcccatgtc gcggccatcg gcaatcaggc ctggtaattc gcgaaacgcg
    19261 cgttggcgac gcaataatgc ttcacgaacg cgtgggaatg ccgcgacttg tgaagcagca
    19321 ttcgccactt cctgagtacg aatttcgccg ctgacatctt ccccttcgag gatcacttcc
    19381 agattgccat tggtcgacac aaaacgtaca tccagatggg atgccagcgg cacgagcgca
    19441 tcttccgatg caacatcaac atggtgatgt aatgccgcca gtgccagtac gcgataaatt
    19501 gcacccgagt ccagcagatg ccattgcaac gcttccgcca tagccttaca caaggtgcct
    19561 ttccctgcac cgcttgggcc atcaatggta ataaccgggg caattgccgt catctttatc
    19621 tccttaaaac aggcgtaccg ttaacataac cgcgcgcatt atacgcgccg acgccacaaa
    19681 tcgttaacgt tgtgtgcaaa aaccagtttt gtctgtattc agatggggat ttattgcgca
    19741 attatagcgg gctggcagaa gaccagcccg aaagatgaca atgatttact taccagaggc
    19801 gatacgatca cggatgtgtt gcgcacgctc tgttgatggt gggtgagaat caaacatgga
    19861 ctgggtacga ccgccatcca ggctggccaa tttttcaaag ctgccaacca gcccctgggt
    19921 gctgatgcca cgtttcttca acagatcaaa ggagaaatca tctgcttcgg actctttatc
    19981 gcgggagtat ttagcattga tcgcgccttc tgcgatatca cctaattgtg agcgagaaag
    20041 ctgggaagcc acaccgctgg tagcggaaat cgcatcacgt gcggcaacga tcgcataaga
    20101 agctttcatt tcagccagcg agtgacccaa cgcgacgtgg cccagttcat gacccagaac
    20161 gccttcaatt tcgttgtcgt tcatcatgtc catcaggcca ctgtagacac gaacacaacc
    20221 gttcgccatc gcccatgcgt tgacgtcgct ggtcatataa accttatagt tgaccggcgt
    20281 gccattaata ttgttaccca atgctttggc gatttttgcc agacgtttcc cgtacttgct
    20341 ggatttgctt gcgacttgat tgccgctgtc catttcgaca cagccctgat tggcgattgc
    20401 tttagcatca gcatcgctta atgttgcggc tttataagca ttcagacctg agctgatagc
    20461 caaatcggta ttaataccgt gcgtattctg acatcctgtc agtaaggctg cagaggtcgc
    20521 aatcgccagc agtaatttag tattcttcat gtgaatcatc cgttgattaa ttatatataa
    20581 acattatgta tcaacaagtt acattgataa ttaacgctac cattaataaa aataccccac
    20641 aaggtccgaa aaaaaacgcc ggcaatgtgc cgacgtcttt gaaaagaaag gattgcctat
    20701 gttatcgccc gttattcatt cagccagatt gacttatccg cgccaattgc tcgaaatagt
    20761 ctggaaaagt tttagcggta cattttggat caagaatcgt cacagccgta tctgataatg
    20821 ccaccagcga gaaacacatc gccatccggt gatcattgta tgtcgcgatc tcggcaaagt
    20881 tcagtttttc cggtggagtg atacgaatgt aatcgtgccc ctcttctact tccgcaccaa
    20941 ctttacgcag ttctgttgcc atcgcaaaca ggcgatcagt ctctttaaca cgccagttat
    21001 agatattgcg cagcgtggtg gtgccttttg caaataacgc cgccgtggca atggtcatcg
    21061 ccgcatcggg aatatggttc atatccatat caatagcgtt cagttcaccc cgcgtgcagg
    21121 aaatataatc atcaccccag caaatggtcg cgcccatttt ttccagcaca tcagcaaagc
    21181 gaatatcacc ctgcatactg ttgcgtccaa tgccggtcac ttttacagtg ccgcctttga
    21241 ttgctgctgc tgccaggaag taagaagccg aagatgcatc gccttcgacc aaataagtac
    21301 ccggagactg ataagactgc ccgcctttta cgacaaattg ttgatagtgc tgattttcaa
    21361 tttcaacacc aaacgtcttc atcagattga gtgtgatgtc gatataaggt ttagaaacca
    21421 gatcgccttt aatacgaata accgtatctt ccggcgcaag aggcgcagtc attaatagtg
    21481 cggtgaggaa ctggctggaa acggagccat caacgtcaac gttgccgcca gtaaagccgc
    21541 cctgtaaacg caatggcgga taattttctt gttccaggta agtgatcttc gccccgccca
    21601 ggcgcagcgc atccaccaga tgaccaatcg ggcgctcttt catacgcggc tcaccagtca
    21661 gcacaatatc attgctaccc agacaaagag ctgccgccag cggacgcatt gccgttccgg
    21721 cgttaccgag gaacaactcc agggcgcttt ttgcgtgtaa tggaccgccg ttaccgataa
    21781 tttcgcaacg cgtacgatca gctgaaagcg tatagcttac ccctaacgct gctaatgcat
    21841 tcagcatatg gcgcacgtca tcgctatcca gcagattggt taatactgtt ttgccgtgtg
    21901 ctaatgccgc cagcaataaa gcgcggttag aaacgctctt ggaaccaggc agattaatag
    21961 tgccatcgac acgagcgatg ggttgtaacg tcagggattc catgaactca actctctaca
    22021 acagaaataa aaaccccaca ggctggctgt ggggattaag caaaatttcg gcattagccg
    22081 tgacggcgtt cgaactcaac catgaagtct gtcagtgctt taacaccttc cagcggcatg
    22141 gcgttataaa tagacgcacg cattccgccg accactcggt gacctttcag cgcatgtaag
    22201 cccgcagcga aagactcttc gaggaacaat ttatcaagcg cgctatccgc caactggaac
    22261 ggcacgttca tccgcgaacg gttagctttc gccacatcat tgcggtagaa atcgctgtta
    22321 tcaatcaccc catacagcag ttctgctttt tgctgattga tcttatccat tgcagccaca
    22381 ccgccgttcg ctttcagcca tttgaagacc aggccagata agtaccaggc aaatgtcggc
    22441 ggcgtgttga acatggaatc gttatcgttg aggatggagt aatccagaat cgatgggcag
    22501 gcgatattcg ctttgcccag caaatcttca cgaacgatga cgattgtcag gccagccggg
    22561 ccgatatttt tctgcgcgcc agcgtaaatt acaccataac ggctgacatc aatcggacgg
    22621 gaaagaatgg ttgaagagaa gtcagcggcg accaccacgt ctttaccgaa gtctggtgtt
    22681 tcatcgatag cgataccgtc aatggtttcg ttcgggcagt aatgcatata agcagcatta
    22741 tcagagagtt gccattcact cattggctta actgcgcgaa gaccatcaac ggtcactttc
    22801 gcatcaaaca catttggtgt gcagtatttt ttcgcttctt tgatagcact tgctgcccag
    22861 taacccgcat caacataatc tgcagtggtt ttatcaccaa gaatattcag cggtacagca
    22921 gcaaattgac cgcgaccacc gccatggcag aacaatacct tgtagttgga ggggacgtta
    22981 agaagatcgc gaaaatcctt ctcggcttcc tctgcaacct gaatgaactc tttgccacgg
    23041 tgactcactt ccatcaccga cgtaccaaga ccgttccagt cgcgcagttc ctgttgagcc
    23101 tgtttaagca cctctgccgg tagcattgcc ggaccagaac taaaattgaa gatttgagcc
    23161 atttcccctc accacgttgc gttgccatta acccgcggtc aatcaattgc gaccacaggc
    23221 aatacattat tcctgtggct atcggtttta tcattcagtg acacgcatcg caatgagtaa
    23281 aaccgttgag ggcaaaaatg aggcacgcgt cacacaaaag aatctctcgg attattttca
    23341 aaaaaccgat gttttggctg tcaggtcacc cgtttagccc gacttgcctt cgtccattct
    23401 ggatttgcac agcgcggaca taattgtttt tcccccgcgt tcatatgaat gatttcactg
    23461 caacggatgc aggcatagtc cgccgactcc ggcaccactt tgtaacgctg agtacagtca
    23521 ctgggtaaaa taagtaaccc cggtttcacc ttgtcatctt caaagaaata aacactgatt
    23581 tgcccgttgg tttcgagaat cgccagacgt acctgcccca actgctctac accgcgcaat
    23641 cgtagctcca taaagaactc aaattccgtc atgttggagt tattgagttt cgaccaggcc
    23701 agctcgccat cttcaataat gacaaccggc ttgccttcca gaagatcttc cagtttttca
    23761 ctgtgcgcca tcaaccacat taccaggcgg tataacaacg ccagagtaat aaagacgata
    23821 agtaccggga ccatcggcac atcatcataa aacgccacat ctcccgccgc tgaacccagt
    23881 gtcagaatga ttaacacttc aaacagcgac atctgccgca caccgcgtct tccggtcatt
    23941 ttgaggaaca aaaaaaccag tacaaaggta taaagactac gtagtgcaac ttcgccaagg
    24001 aaatcaaaag gcactttatc aaatgccata cggtggagat cgaacgcctt catttttatg
    24061 ctcttatcgt ggtttatccg ctaaaagcat agccaacagt tcatcggcat ggcggcggta
    24121 atagcgcctt tgagataaaa cccgtattat tgtgcgcttt ccgtacgact aaagtgattt
    24181 tcatgacgca aacatttatc cccggcaaag atgccgctct ggaagattcc atcgcacgct
    24241 tccagcaaaa actttcagac ctcggttttc agattgaaga ggcctcctgg ctgaatccgg
    24301 tgcctaacgt ctggtctgta catattcgcg acaaagagtg cgcactgtgt tttaccaacg
    24361 gtaaaggcgc aaccaaaaaa gcggcgctgg cttctgccct cggtgaatat ttcgagcgtc
    24421 tctcaaccaa ctactttttt gcggacttct ggctgggcga aaccatcgcc aacggcccat
    24481 tcgtgcatta tcccaacgaa aaatggttcc cactgaccga aaatgacgat gtaccagaag
    24541 ggctgctcga tgaccgtctg cgcgcatttt acgatccgga gaatgaactg accggtagta
    24601 tgctgattga cctgcaatcc ggtaacgaag atcgtggtat ttgcggtcta ccgtttacgc
    24661 gccagtccga caatcagacc atttatattc cgatgaatat cattggtaac ttgtacgttt
    24721 ctaacggtat gtctgctggc aatacccgta acgaagcacg cgttcagggg ttgtccgaag
    24781 ttttcgaacg ttacgtgaaa aaccgcatta ttgctgaaag catcagcttg ccggaaatcc
    24841 cggcagacgt gctggcacgt tatccagcag tagttgaagc gatcgaaacg ctggaagcgg
    24901 aaggtttccc gatcttcgct tatgacggtt cgcttggcgg ccagtatccg gtgatttgcg
    24961 tggtactgtt caatcctgct aacggtacct gctttgcctc attcggtgcg catcctgatt
    25021 ttggcgttgc actggaacgt accgtgaccg agttgctgca aggtcgtggc ctgaaagatc
    25081 tggatgtgtt tactccacca accttcgatg atgaagaagt cgctgaacat accaacctcg
    25141 aaacgcactt tatcgattcc agcggtttaa tctcctggga cctgttcaag caggatgccg
    25201 attatccgtt tgttgactgg agcttctccg gcaccacgga agaagagttc gccacgctga
    25261 tggctatctt caaaaaggaa gataaagaag tttatattgc cgattacgag catctgggtg
    25321 tctacgcttg ccgtattatc gtgcctggca tgtccgatat ttatccggct gaagatctgt
    25381 ggctggcgaa taacagtatg ggcagccatt tacgtgaaac gattctttcg ctaccaggta
    25441 gcgagtggga aaaggaagat tacctgaacc tcatcgagca actggatgaa gaaggttttg
    25501 atgactttac ccgcgtgcgt gaactgttgg gtctggcgac cgggtcggat aacggctggt
    25561 acactctgcg tatcggtgaa ttaaaagcca tgctggcgct ggctggtggc gatctggaac
    25621 aggctctggt ctggaccgaa tggacgatgg agtttaactc atcagtattc agcccggaac
    25681 gcgccaatta ttatcgctgc ctgcaaacgt tgttattact ggcgcaggaa gaagatcgcc
    25741 agccgctgca atatctgaat gcgtttgttc gcatgtacgg cgcagatgcc gtggaagccg
    25801 ccagtgcggc aatgagcggc gaagcggcat tttatggctt gcaaccagta gatagcgatc
    25861 tgcacgcgtt tgctgcacat cagtcgttgt tgaaggccta cgaaaagctg caacgcgcca
    25921 aagcagcatt ctgggcaaaa taaaatcaaa tagcctacgc aatgtaggct taatgattcg
    25981 tctgagttat attacggggc gtttttt
//
LOCUS       DAPHCV010000075        24863 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.075, whole genome shotgun sequence.
ACCESSION   DAPHCV010000075 DAPHCV010000000
VERSION     DAPHCV010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.075"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            123..2366
                     /gene="pdeF"
                     /locus_tag="RH033_003027"
     CDS             123..2366
                     /gene="pdeF"
                     /locus_tag="RH033_003027"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HDV2992179.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDSRLISQRLFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDVKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREVVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2405..3946)
                     /gene="ppx"
                     /locus_tag="RH033_003028"
     CDS             complement(2405..3946)
                     /gene="ppx"
                     /locus_tag="RH033_003028"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HDV2992180.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(3951..6017)
                     /gene="ppk1"
                     /locus_tag="RH033_003029"
     CDS             complement(3951..6017)
                     /gene="ppk1"
                     /locus_tag="RH033_003029"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HDV2992181.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6189..6827)
                     /gene="purN"
                     /locus_tag="RH033_003030"
     CDS             complement(6189..6827)
                     /gene="purN"
                     /locus_tag="RH033_003030"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDV2992182.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLITSAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6827..7864)
                     /gene="purM"
                     /locus_tag="RH033_003031"
     CDS             complement(6827..7864)
                     /gene="purM"
                     /locus_tag="RH033_003031"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDV2992183.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8189..8815
                     /gene="upp"
                     /locus_tag="RH033_003032"
     CDS             8189..8815
                     /gene="upp"
                     /locus_tag="RH033_003032"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDV2992184.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8901..10190
                     /gene="uraA"
                     /locus_tag="RH033_003033"
     CDS             8901..10190
                     /gene="uraA"
                     /locus_tag="RH033_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDV2992185.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISMLRPEEVVLDAEDADITDK"
     gene            10240..10986
                     /gene="hda"
                     /locus_tag="RH033_003034"
     CDS             10240..10986
                     /gene="hda"
                     /locus_tag="RH033_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HDV2992186.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(11092..11451)
                     /gene="arsC"
                     /locus_tag="RH033_003035"
     CDS             complement(11092..11451)
                     /gene="arsC"
                     /locus_tag="RH033_003035"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HDV2992187.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11472..12935)
                     /gene="bepA"
                     /locus_tag="RH033_003036"
     CDS             complement(11472..12935)
                     /gene="bepA"
                     /locus_tag="RH033_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HDV2992188.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTSTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPIVVQSSEDFYL
                     AKVRTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13148..14209
                     /locus_tag="RH033_003037"
     CDS             13148..14209
                     /locus_tag="RH033_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDV2992189.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQD"
     gene            complement(14308..14778)
                     /gene="bcp"
                     /locus_tag="RH033_003038"
     CDS             complement(14308..14778)
                     /gene="bcp"
                     /locus_tag="RH033_003038"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDV2992190.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14778..15350)
                     /locus_tag="RH033_003039"
     CDS             complement(14778..15350)
                     /locus_tag="RH033_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HDV2992191.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            15496..16374
                     /gene="dapA"
                     /locus_tag="RH033_003040"
     CDS             15496..16374
                     /gene="dapA"
                     /locus_tag="RH033_003040"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDV2992192.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            16391..17425
                     /gene="bamC"
                     /locus_tag="RH033_003041"
     CDS             16391..17425
                     /gene="bamC"
                     /locus_tag="RH033_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HDV2992193.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17638..18351
                     /gene="purC"
                     /locus_tag="RH033_003042"
     CDS             17638..18351
                     /gene="purC"
                     /locus_tag="RH033_003042"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDV2992194.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            18508..18735
                     /gene="vapB"
                     /locus_tag="RH033_003043"
     CDS             18508..18735
                     /gene="vapB"
                     /locus_tag="RH033_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HDV2992195.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            18735..19133
                     /gene="vapC"
                     /locus_tag="RH033_003044"
     CDS             18735..19133
                     /gene="vapC"
                     /locus_tag="RH033_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HDV2992196.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            19280..20143
                     /locus_tag="RH033_003045"
     CDS             19280..20143
                     /locus_tag="RH033_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HDV2992197.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            20158..22173
                     /gene="tmcA"
                     /locus_tag="RH033_003046"
     CDS             20158..22173
                     /gene="tmcA"
                     /locus_tag="RH033_003046"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HDV2992198.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCFPSALQTLLGREFRHAVFDARHGFDAASFAALSGTLKAGSWL
                     VLLLPIWDEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTANNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLYQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTQDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAVLSDDDWLELAGFAFTHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKLLLVRQREEAAQALFALDDVRTERLRDRITQWQFFH"
     gene            22247..22945
                     /gene="ypfH"
                     /locus_tag="RH033_003047"
     CDS             22247..22945
                     /gene="ypfH"
                     /locus_tag="RH033_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDV2992199.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQLALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(23026..23226)
                     /locus_tag="RH033_003048"
     CDS             complement(23026..23226)
                     /locus_tag="RH033_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HDV2992200.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(23254..24381)
                     /gene="dapE"
                     /locus_tag="RH033_003049"
     CDS             complement(23254..24381)
                     /gene="dapE"
                     /locus_tag="RH033_003049"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HDV2992201.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVASERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(24385..24741)
                     /locus_tag="RH033_003050"
     CDS             complement(24385..24741)
                     /locus_tag="RH033_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HDV2992202.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAATLMTEMPAIIKRPLLCAPGKPM
                     LLGFSESSYQQFFHEV"
BASE COUNT         5840 a         6164 c         6533 g         6326 t
ORIGIN      
        1 actttcatat gacacttaga atcattgctc tctatagtga tgaataatca tcattcgaag
       61 tcaggtggga tgcctgtctg aatacacctc cttcaggatg tgggggattc tgctgagcat
      121 ctatgaaact gaatgcaact tatataaaaa tacgtgataa atggtggggg cttccgctgt
      181 tcctgccttc tttaatcttg cccattttcg cccacattaa tactttcgcg catatttctt
      241 ccggtgaagt ttttctcttt tatctgccac tggcactgat gatcagcatg atgatgtttt
      301 tcagctgggc ggcattgcca gggatcgcct tagggatttt tgtccgcaaa tatgcagagc
      361 tgggttttta cgaaacgctc tcattaacgg ctaattttat tatcattatc attctctgtt
      421 ggggcggtta cagggttttt actccccggc gtaacaacgt ttcacatggt gatagccgtt
      481 taatttccca gcgactattc tggcagattg tgtttcctgc aacgctgttt ctgatacttt
      541 tccagtttgc tgcgtttgtt ggattactgg cgagcagaga aaatctggtc ggcgtcatgc
      601 cctttaacct cgggacctta atcaattatc aggccttgct ggtgggtaat ctgattggtg
      661 tcccgctgtg ctacttcatc attcgtgtag tgcgaaatcc gttttattta cgtagttatt
      721 attcgcaatt aaaacagcag gttgatgtca aagtcaccaa aaaagagttc gcactctggt
      781 tactggcatt aggtgcttta ctgctgctgt tatgcatgcc gttaaatgaa aaaagcacaa
      841 tttttagcac caactatacc ttgtcattat tgctgcccct gatgatgtgg ggagcgatgc
      901 gctatggtta taagctgatt tcgctgctct gggcggtcgt gttgatgatc agcatccaca
      961 gctatcaaaa ttacattccc atttatcctg gctataccac gcagctgacc ataacctcct
     1021 ccagttatct ggtattctct tttattgtca attatatggc tgtactggca acccgtcagc
     1081 gagcggtagt cagacgcatt cagcggcttg cgtatgtgga cccggtggtt catctgccaa
     1141 atgttcgcgc cctgaatcgc gcgttgcgtg atgccccctg gtctgcgctt tgttatttac
     1201 gcatccctgg catggaaatg ctggttaaga actatggcat catgctacgg attcaataca
     1261 agcaaaaact ttctcactgg ctgtcaccct tgctggaacc gggtgaggat gtttatcagc
     1321 tttcgggtaa cgatctcgcg ctgcgactga atacagaatc gcaccaggag cgcattaccg
     1381 cactggatag ccatctcaag caatttcgtt tcttttggga tggaatgccg atgcaaccgc
     1441 agattggcgt cagttactgc tatgtgcgct cgccagtgaa tcatatctac ctgctgctgg
     1501 gagagctaaa tacggtcgcc gaactttcca tcgtgacgaa cgccccggaa aatatgcagc
     1561 gtcgcggggc aatgtatttg caacgcgaat tgaaagataa agtcgcgatg atgaatcggc
     1621 tacagcaggc gctggaacac aaccattttt tcctgatggc ccagccgatt accggtatgc
     1681 gtggcgatgt ctaccatgaa attcttctgc gcatgaaagg tgagaatgat gaactgatca
     1741 gccccgacag cttcttgccg gtcgcgcacg aatttggttt atcgtcgagt atcgacatgt
     1801 gggtcattga gcatacgctg caatttatgg ctgaaaacag agcgaagatg cccgctcacc
     1861 gttttgcgat taatctgtcg ccaacctcgg tatgtcaggc tcgttttccc gttgaagtca
     1921 gtcagctgct ggctaaatat caaattgaag cgtggcaact tatttttgaa gtcaccgaaa
     1981 gtaatgctct gaccaatgtt aagcaggcgc aaatcacctt gcagcatctt caggaattag
     2041 gctgccagat tgcgattgat gatttcggca ccggctatgc cagctatgcg cggcttaaaa
     2101 atgtgaatgc cgatttgctt aaaattgacg gcagttttat ccgcaatatt gtgtcaaata
     2161 gtctggatta tcagatagtg gcgtcgattt gccacctggc gcgaatgaag aaaatgcggg
     2221 tagtggcaga gtacgttgaa aacgaagaga tccgcgaggt ggtgctctct ttggggatcg
     2281 attatatgca gggttatctt attggtaagc cgcaaccgtt aattgatacg ctgaatgaaa
     2341 tcgaacccat tcgcgaaagt gcctgaataa tgcgggccga catttctcgt cggcccgcaa
     2401 agcattaagc ggcgatttct ggtgtacttt cttcttcaat tttcaaccgc cagccagcca
     2461 cgccttccca gtattcttgc tccttttcca gatcaagcag taccagcgca ttctgactaa
     2521 accagtcatg cggaaaacgc agtgtccagt gactgtcatc ggtaatcagc gtcaatgttg
     2581 gcggtgtggt ggttgcctga cgttgattat tgagtaatac gccaaggcgc aatagctgaa
     2641 tcagtggcag gaactgtttc ttcttaaaca aggtaaagcg cggcagatcg tcgagcttaa
     2701 tcgctttacg gtgatagcgc accagcgtcg ccatcatcaa ctgctgttcc tgattaaaac
     2761 ccggcaagtc gctgttttgc agaatataag cggagtggcg atgcaaaccg ctgtggttga
     2821 tattcaaccc gacctcatgc agcatggcgg cccatcgcag tagcgcctcc agttgcggat
     2881 gcgccagctt cggttgctgt tcccgccact gttcgtacat ttgcatagtg gtatccagca
     2941 cccgtcgggc ctgttcactg tcgatgtgat actggttggc gaggctgctg gcggtgcgac
     3001 tacgcacatc ctgatgacgg aaacgtcctt ccatttcata cagtacgcct tcgcgtaacg
     3061 ccccgtcaga aaggcgcagt tcacggatgg ctaaagcatc aaacacaccg cataaaatcg
     3121 ccagtccagg aacgaagact gttttccgct cttcggaaag acctggtaag ctcagcgatg
     3181 cgaaattacg gtgccgtaaa acttctttta ccagtttttc cagacgttcc ggggtaatta
     3241 tcccgtcttt ctcgcccatt tccattaaca cttcatgggc ggcttttatg gtaccggaag
     3301 cgcccattgc cacgttccag ccctgaatac ggaattgcca ggttaaagtt tccagttttt
     3361 gtgctgccgc catgcgagcg cgctgaaaat tctctttatt gatgaccccg ccagggaaat
     3421 aaagctgggc aaagctgaca caacccatcc ggcggctttc aacgagaatc ggttcgaaat
     3481 tttccccaat caccagttct gtagaaccgc cgccaatatc aataaccagt ttgcgacctt
     3541 tctccggttg ggtatgttcc acgcccataa aaatcagacg ggcttcttca ttaccggaaa
     3601 taatttcaat cgggtaggga atgacctttt ccgcgcgttt cagaaagtca gtggcgttca
     3661 gcgcctgacg cagtgtatgg gtgccaacta tacagacgct ggcaggagaa aacccttgca
     3721 gccgttcggc aaacagcgac agacagttta aaccgcgcgt cattgcctct tcactcaaca
     3781 tattatctgg ccccaggccg tccgccagat gcactcgctg tttcaggcgg ccaataatct
     3841 gcatggcacc atctaccaca cgggctatga ccatgtgaaa actgtttgaa ccaagatcga
     3901 ccgcagcaaa ctcctgcgga cgaggggatt tatcgtgtat tggcataggg ttattcaggt
     3961 tgttcgagtg atttgatgta gtcgtaaatc gccaactgcg cccgtacttt acggcgattg
     4021 ccgcggggaa cgtagcgatt actgagttct ttatcgatat aacgtgcttt gaccgtatcg
     4081 ctgaacaata tgtcgatgat gtccagcacc cgctgcttca ggcgcggatc gagcagcggt
     4141 gtcgccactt caatacgata atcaatattg cgcgtcatcc agtcggcgga agaaaggtag
     4201 acctttttat cgccgccatt ttcaaaaata taaacccggt catgttcaag gtaacggtca
     4261 acaatactga tggcacgaat gttgtcgcta atgccttcca gattggggat cagcgaacac
     4321 atgccgcgaa ccagcagatt aaccggtacg ccggagctgg aggccgcata cagacgatca
     4381 accaggcctt tatcgacaag gttatttagc ttcagggtga taccgctggg cagcccttgc
     4441 tgcgcgttgg cgatttcgcg gtccaccatt tcatacaaca ggcggcggga gttttgcggc
     4501 gacaccatta aataatcaaa tgtcaccgga cggtatgggt tttcaataaa gttgaacacc
     4561 cgccgtactt cgttggttat gcgcgcatcg gcggtcagca acgaatagtc agtataaaga
     4621 cgcgcggttt tttcgttaaa gttcccggtc ccgatgtgtg cgtaacgcac cacttcaccg
     4681 ttttctttac gtgaaatcag gaacagtttg gcgtgaattt tcagccccgg cgcagagaag
     4741 ataacgtgca cgcctgcttc ggtcaggcgc ttcgcccagt gaatgttggc ttcttcgtcg
     4801 aaacgcgcct gtaactcaac caccacggtg actttcttac cgttgtgtgc ggcgtggatc
     4861 atcgagtcga tgatgcgtga atctttcgcc acgcggtaaa tgttaatttt aatcgccagt
     4921 acgctcgggt cgaacgaagc ctgacgcagc agttccagta catgctcaaa ggtatgataa
     4981 ggataataga gcaacacatc gcgttcgcga atagcatcaa aaccattgcg gaactgggct
     5041 ttatcaaacc aaatgtggcg taaacgcggc agtggtttgt tcaccagatt ggctttgccg
     5101 acattgggga aattaataaa gtctttaaaa ttatgataac gaccaccggg gacgatggag
     5161 tcgtagcggg aaatagtcag tttttcgcgt aacacttcaa ccagcgcatt gggcatatcg
     5221 cgctgataaa caaaacgcac cggctcagca gttaaacgct gcttgagact ggaagacatc
     5281 aactccatca ggctggcttc catctcatgc actaaatcgt attcggcatc gcgggtcatc
     5341 ttcattgaat aggcattcag cgcgtcataa tcaaagaagc ctttgaaaat atcatcgagg
     5401 cagtaacgca gaatgttatc cagaagaatc atcggcttgc gtcgacgtgg cgcttctggc
     5461 ggtaaattca caaagcgcgg cactttatct gatgggatct ccagcagcgc gtaacggatg
     5521 gtatcgccac ggataatttc caccgccaga taggtgtaat catctttcag gaactgcact
     5581 aagtcagtgt caggattgat taaaatcggc gtaatgtgct gacgcagata ctgcttaaaa
     5641 taatgacgca gccagttttg ttgattgacg gagagctggc gttcattaat caggaagatc
     5701 tggttgcgcg ccatctccag caacagctcg ttgtagaggc cgtcgaattc ctgatcggct
     5761 ttcagcaccc gggactgaat tttgcccagt aaatggcggg aatgagagtt ggaaccttgt
     5821 tcttcgctaa taatgatgcg tcgcttcagt tcagcgaagc ggactttata gaactcatca
     5881 aggttattgg aatagatccc caggaaacgc atcctttcaa tcagcgggtt agatttgtcc
     5941 gccgcttcct gaagcacgcg ttcattgaac gataaccaac tgagctcttt ttcgatgtat
     6001 agcttttcct gacccattac cacttttact ccgttttatt cacgggacac tgcctggata
     6061 ttatggcgag caatattggc agatgtccaa ctatgccaga aaagggggaa aaaaactgct
     6121 ggagcttgcg atttacaggt gaattgaaaa acgccagcgg cagagctggc gctttaacta
     6181 cgggggcatt actcgtcggc agcgtagccc tgcggcggca gacgttgacc atccagccac
     6241 gcggcgtttt cgtgcatttt cagacgacca tcggcaaacc agctaatcac cagtggataa
     6301 atagcgtgtt cctgggtttg cacgcgggcg gtgatgtcat cttccgtatc accagcaaat
     6361 accgggactt tcgcctgtaa aataaccggg ccaccgtcca gttcatcggt gacgaaatgc
     6421 accgatgtac cgtgctcctc atcgccattt tccagcgcct gacgatgggt gtgtaatccg
     6481 ggatatttcg gcagcagaga agggtgaatg ttcagcaaac gcccggcata gtgggagaca
     6541 aacgccgggc tgagaatgcg cataaaacca gccagcacga ccacatcggg tgcgtacatg
     6601 tcgatttcat gaatcaactc ccggtcatag gcttcacgac tgtcaaacgc gctggtgatg
     6661 agcgtatgcg ttgcaatacc cgcctggcgg gcgcgttcaa ggccgaacgc gtcggcctta
     6721 ttgctgaaaa ctgcccgtac ggtgccttta attttgttgg ttttgcaggc gtcaataatt
     6781 gcctgcaaat tacttccgtt gccggaaata agcaccacaa tattcattat tcgataacca
     6841 cgcgttgttc ggaatcagag gctttgatga taccgatttt ccacgcgttt tcaccgttgg
     6901 cattgagcag ggcgagagct ttgtccactt ccggagcagg cagggcaata atcataccga
     6961 cgccgcagtt gaaggtgcga tacatttcat ggcgctcaac attacctgcc gtttgcagcc
     7021 agttgaacac ttccggccac tgccaggaag attcatcaat cactgcctgg gtattatctg
     7081 gcaatacgcg cggaatgttt tcccagaagc cgccgccggt caggtgcgca atggcatgca
     7141 catcgacctt ttcaatcaac tccagcactg acttcacgta aatgcgggtc ggtgccagca
     7201 gatgatcggc taatggctta ccatcaagtt cggtggtttg cggatcacaa ccgctgactt
     7261 caagaatttt gcgcaccagc gaatagccat tcgagtgcgg gccgctagag ccgagtgcaa
     7321 tcagcacatc gccgtcgctg actttagatc cgtcgatgat ttctgatttt tctaccacgc
     7381 caacgcagaa accagcgaca tcgtaatctt caccgtgata catccccggc atttctgccg
     7441 tttcgccacc caccagtgaa cagcccgatt gcagacaacc ttccgcgatg ccgctgatca
     7501 ccgctgaagc ggtatcaaca tccagttttc cggttgcgta atagtcgagg aaaaacagcg
     7561 gttccgcacc ttgcaccacc aggtcattaa cgcacatggc gaccagatca ataccaatgg
     7621 tgtcgtgacg ttttaagtcc atcgccagac gcagcttggt acctacgccg tcagtgccgg
     7681 aaaccagcac aggttcacga tatttttgcg gcaatgcaca cagtgcaccg aagccgccca
     7741 gaccgcccat cacttccgga cgacgcgttt tcttcactac gcctttgatt cttccaacca
     7801 gagcattacc cgcgtcaata tcaacaccgg catctttgta gctaagagag gttttatcgg
     7861 tcactgcttg ggtccccacg cgttacttgc ggtagaaaaa taaaattcgg cgcaattcta
     7921 acagggaaag caaacgtttg cgagactgct ttacacaacc tttttgcacg tcttttcccc
     7981 aggcgcgcgg cgaaagaata cttgtactag ggcaaaggat cgttttcgga tggaataatc
     8041 ttcttccata accatttgaa tataaaataa ctttatctca aaccgttatc attttgacta
     8101 aagtcaacga aaataatatt gccgccttga agaaaggagg tataatccgt cgattttttt
     8161 tgtggctgcc cctcaaagga gaaagagtat gaagatcgtg gaagtcaaac acccactcgt
     8221 caaacacaag ctgggactga tgcgtgagca agatatcagc accaagcgct ttcgcgaact
     8281 cgcttccgaa gtgggtagcc tgctgactta cgaagcgacc gccgacctcg aaacggaaaa
     8341 agtcactatc gaaggctgga acggcccggt agaaatcgac cagatcaaag gtaagaaaat
     8401 taccgttgtg ccaattctgc gtgcgggtct tgggatgatg gacggtgtgc tggaaaacgt
     8461 tccgagtgcg cgcatcagtg tcgtcggtat gtaccgtaat gaagaaacgc tggagccggt
     8521 accgtacttc cagaaactgg tttctaacat cgatgagcgt atggcgctga tcgttgaccc
     8581 aatgctggca accggtggtt ccgttatcgc gaccatcgac ctgctgaaaa aagcgggctg
     8641 cagcagcatc aaagttctgg tgctggtcgc tgcgccagaa ggtatcgctg cgctggaaaa
     8701 agcgcacccg gacgtcgaac tgtatactgc atcgattgac cagggactga acgagcacgg
     8761 atacattatt ccgggcctcg gcgatgccgg tgacaaaatc tttggtacga aataaagaat
     8821 aaaaataatt aaagccgact ttaagagtcg gctttttttt gagtaaagcg cctataacac
     8881 ataatacaga ggataatact atgacgcgcc gtgctatcgg ggtgagtgaa agaccgccac
     8941 ttttacagac aatcccgctt agtttgcaac atttgttcgc catgtttggt gcaaccgtcc
     9001 tggtgcccgt cttatttcat attaacccgg cgactgtact gttatttaac ggtataggaa
     9061 cactgctgta tctcttcatc tgtaaaggga aaattccggc ttatcttggt tccagctttg
     9121 cctttatttc accggtattg ttactgttgc cgttggggta tgaagttgcg ctgggcggct
     9181 ttattatgtg cggcgtgctg ttctgcctgg tttcttttat cgtgaagaaa gcggggaccg
     9241 gctggctgga cgtgctgttt ccacctgcgg caatgggcgc aatcgttgcc gtcatcggtc
     9301 tggagctggc gggcgtagct gccggtatgg cgggtttact cccggctgaa gggcaaacgc
     9361 cagactccaa aaccatcatc atctctatta ccaccctggc ggtcacggtt ttaggttccg
     9421 tgctgtttcg tggtttcctg gcaattatcc cgattttaat tggcgtgctg gtggggtacg
     9481 cgctctcttt cgcaatggga attgtcgata ccacgccgat tattaatgct cactggtttg
     9541 cgctgccgac cctctatacg ccgcgcttcg agtggtttgc cattctgact attctaccag
     9601 cggcgttagt ggttattgcc gaacacgtag ggcacctggt cgtaacggct aatatcgtca
     9661 aaaaagatct gctgcgcgat ccaggtctgc accgttcgat gtttgctaac ggcttgtcga
     9721 ccgtgatctc cggcttcttt ggctccacgc caaacaccac ttacggagaa aacattggcg
     9781 tgatggcgat cacccgtgtt tacagtacct gggttatcgg cggtgcggcg attttcgcta
     9841 tcctgctttc ctgcgtcggt aaactggctg cagctatcca gatgattcca ttgccggtga
     9901 tgggcggcgt ttcgctgctg ctttacggtg tcatcggtgc ttccggtatt cgtgtgttga
     9961 tcgaatcgaa agtggattac aacaaagcac agaacctgat cctgacttcc gttattttga
    10021 tcatcggcgt cagtggcgcg aaggtaaaca ttggcgcggc ggagctgaaa ggtatggcgc
    10081 tggcgaccat cgtcggtatc ggcttaagtt tgatcttcaa actgattagt atgctgcgcc
    10141 ctgaagaagt ggtgctggac gcagaagacg cggatataac agacaaataa tgtaccgacc
    10201 gggcagtgtt gctgcccggt tcaaacatca tgggattctg tggtaaactt ctcgcgattt
    10261 tgtgaaatcc tggttgaggt atctctgaac acaccggcac agctctcttt gccactttat
    10321 cttcctgacg acgaaacctt tgcaagtttc tggccggggg ataactcctc tttactggcc
    10381 gcgctgcaaa acgtgctgcg tcaggaacat agcggttaca tctatctctg ggcacgcgaa
    10441 ggcgcggggc gcagccatct gctgcacgcg gcttgcgcgg aattgtcgca gcgtggcgat
    10501 gcggtgggct atgtcccgct ggataaacgc acctggtttg ttccggaagt gctcgacggt
    10561 atggagcatt tgtcgctggt ctgtatcgac aacattgagt gtattgcagg cgatgagttg
    10621 tgggagatgg cgattttcga tctctacaat cgaattctgg aatcgggcaa aacacggttg
    10681 ttaatcaccg gcgatcgtcc accgcggcag ttgaatcttg gattaccgga tctcgcgtcg
    10741 cgtctcgact gggggcagat ctacaaattg cagccacttt ctgatgaaga taagttgcag
    10801 gcgctacagt tacgcgcgcg tttgcgtggt tttgaactgc cggaagatgt ggggcgtttc
    10861 ttgctgaagc ggctcgacag agagatgcgc acgttattta tgacgttgga tcagttggat
    10921 cgtgcgtcga ttaccgcgca acgtaagctg accattccgt ttgtgaaaga aattctgaaa
    10981 ctgtagacaa gacgtttatt gtcggatatg cacccatagt attcgtcggc cggataagac
    11041 gcggcaagcg tcgcatccgg caatgtgctt caactagcga tggagcactt cttaacccac
    11101 gatttccagt acctgctccg gtggacgacc aatccgcgct ttgccattcg cgaccacaat
    11161 cgggcgctcc attagcttcg gattatccac cattgcctga atcagcgcct cttcgctaag
    11221 cgaactgtca gccaggttca gttctttata aagatcctct ttctggcgca tcaattcacg
    11281 ggcgctattc atccccagca ttttcagcaa atcacgcagc gttgccgcat cggcgggtgt
    11341 ctcaaggtag agcaccactt ctggctccac gccgttttct ttcagcaggt tcagcgtttc
    11401 ccggctcttt gaacagcgtg ggttatggta gatttttacc tgtttggtca ttgttcttcc
    11461 tttaatgcga attacatctt ggtataaggc ttaaagcgtt cctgcagctg gcgcaactgg
    11521 tcgatgcgcg catcgtaacg cgcttgttgc aggctgccta atttcacctg cgaactggcg
    11581 ctactcaaca gcgaaatggc ctgatcgagt cgtccggcga gcgcataacc ttctgctcgc
    11641 gcagccagct cctgatcgcg gttatttagc gcggcttccg cctgtgccag caaatcccag
    11701 ccgttgctgt catctttatt attaaaggtg tagcgattca gaatattggc cgcttcttgt
    11761 ggttgaccgc cttgcagata agcgttcgcc aggttgagct gcaacaccgg attggtgcgt
    11821 aaatcgcggg catttttcag acgattgatc gcctcattgg ctttgttttg cccaagatcg
    11881 atatcagtag ccagatcgag ataccatgcg ttgccaggtt ctgccgccag taacggttgc
    11941 agcgttttac gcgcctcgtc gtatttattg gcttccatcg cctgtagagc acgaccatat
    12001 tgcgccgctc gttgctgacg aacgtttcct ttcgcccatt catccagcaa atcactggtg
    12061 agctggttac gtccggaatt atacatcccc agtgtgcgca ctttcgccag atagaaatct
    12121 tctgacgatt gcaccactat cgggcgcatc tgattagcac ggttgcgggc atctgccaga
    12181 cgactttccg gcaacgggtg agtcaataaa atttccggcg gacgcgagga gtaacgcgcc
    12241 tgatcgagta atttttcgag gaaggtcggc atcgcctgcg gatcgaatcc cgagcgttgc
    12301 agcacctgaa taccaatgcg gtccgcttcc tgttcatttt gctgggtgaa actgatcatc
    12361 ccctggcgcg ttcccgccag tgtactggtc agcgccgcca tccccgcctg cggactggcc
    12421 atcgcaagta aaatagaacc taacgcgccg acccaggtca gcggcgcgct gcgctgctga
    12481 tcttccatcg ctcgcgccag gtgacgttgg gtgacgtggg agatttcgtg cgccataact
    12541 gaagccagtt gactttcgtt atcggaataa cggaacaggg cagagtgcag caccacgttg
    12601 ccgccaaaga aagcaaaggc gttaatttca tcgttgttga tcagaaagaa atgaaacggt
    12661 gtcttaaccg aattggcatg cgaaaccaga cgcattccta gcgaattaat gtattgcgtt
    12721 aacagcggat cattaattaa cggcgcgctg ccgcgtagct ggcggacata atagtcgccc
    12781 atctgcattt cctgaccaat ggaaagtgtg cttcctgcgg aggttcccat atccggcaag
    12841 gtgtctgcgc tgtcggcaaa cgccggggct acctgaccaa tggtcatagc agcaatgagg
    12901 gttgcaacca ggtttttttt caactgcctg aacataacct ctatcctgta tttctggaga
    12961 tagtcatttg accgatactg tgaaatatcg ttcccaccgc gccgcctttg agtgtaacgg
    13021 cgaagattag cccactcaaa tgacttttca gtttcctgaa tccgtcataa aaagcgaagt
    13081 cttttttgtg gcatttcgat acaattgcgg atcgcaatcc cgctcttgag gttcagggaa
    13141 ggtctgtatg ctcgaaatgt tgatgcaatg gtatcgccgc cgttttagcg acccggaagc
    13201 gattgccttg ctggttattt tggttgctgg ttttggcatt atctttttct ttagcggcct
    13261 actcgcgccg ttgctggtgg ctattgtgct ggcctatttg ctggaatggc cgactgtccg
    13321 cctgcagtct attggctgct cccgccgctg ggcgacgtcg attgtattgg tggttttcgt
    13381 cggtatattg ctactgatgg cgttcgtggt actgcctatc gcctggcagc agggcatcta
    13441 cttaatccgc gatatgccgg ggatgctcaa taagctttct gactttgccg ccacgttgcc
    13501 gcgccgctat ccggcgttaa tggatgctgg cattattgat gcaatggccg aaaatatgcg
    13561 cagtcggatg ctgaccatgg gcgattcggt ggtgaaaatt tccctcgcct cgctggtcgg
    13621 tttgctgacc attgccgtct atctggtgct ggtaccattg atggtcttct tcctgctgaa
    13681 agacaaagag cagatgctga acgccgttcg ccgggtgctg ccgcgtaacc gtggactggc
    13741 aggacaggtg tggaaggaga tgaatcaaca aatcaccaac tatatccgcg gcaaagtgct
    13801 ggagatgatc gtggtgggga ttgccacctg gctgggattc ttactcttcg ggctgaacta
    13861 ttcgctgctg ctggcggtgc tggtcggctt ctcggtactt attccgtaca ttggtgcatt
    13921 tgtggtgacc attccggtgg ttggcgtggc gctattccag tttggtgctg gcacggaatt
    13981 ctggagctgc ttcgcggtgt atctgatcat tcaggcgctg gacggcaacc tgttagtacc
    14041 tgtgttgttc tccgaagcgg ttaacctgca tccgctggtc attattttat cagtagtgat
    14101 cttcggtggt ttgtggggat tctggggcgt attcttcgcc attccattgg cgacgctgat
    14161 caaagccgtg attcacgcct ggcccgatgg gcaaatcgcg caagactgac cttcagcgaa
    14221 aaaatttgaa acgagtgttg cagggcagtg agaagatgtg agatgtgctg gccgcaagcg
    14281 cagccagcac ggaatggagc aaagtaatca ggcgtgttct ttcagccagt tcagcacaac
    14341 gtcgtggtga ttgctggttt tgaaatcgtc aaagacatgt tcgattttgc catcagcgtc
    14401 aatcaggaag ctgatgcgat gaataccatc gtaggttttg cccatgaagg acttttcacc
    14461 ccagacgccg aattgttcgc acacctggtg gtcctcatca gacaggagcg taaagttaag
    14521 cagctctttt tccgcaaaac gggagagttt ttcgggttta tcggtgctga tacccagcac
    14581 atcaacgccc gcttttttca actcatccat gttatcgcgt aagccgcagg cctgtacggt
    14641 acagccgggg gtcatggctt tcgggtagaa ataaaccaga acacgctgtc cctggaagtc
    14701 ggtcaaatta acttgttctc cgtcttgatc cggcaagcta aatttcggtg cgatatcacc
    14761 ggctttcagt ggattcatta cttaactcca tcctgttcat catgttggga ataattgacg
    14821 acgttaatac tgccttgtgc attgagttct gtacataggg ctttgaacgc ttgctcaata
    14881 tttgctgcgt ccgcagatgc ggggctgtgg gcagttatct gaatatgcaa ctgcgcagcc
    14941 cgttcatttt cagcaggttg cgtgcgcgac accagctccg caatgttcat atgatgcgcg
    15001 tcgaaaagtg ctgtgaagcg ttcaattaaa tgcggggagt ctgccacatc gacctgaacc
    15061 cagacagatg ctggcattgg cggacgcgga cgcgccgtcg tgcgcttcat cacgattaaa
    15121 agatccagct cggcaccttt caacggtaac gttgattcaa tcagagtaat ggcattccat
    15181 gaaccggaaa gcagcatgat aaacgtgaac tcttctccca gcatcgccag gcgactgtct
    15241 tcaatattac agccgcaact actgacatga cgggtgatgg tgttcactat tccagggcga
    15301 tcggcaccca acgcagtgat caccagataa tgttgcgatg acagtgtcaa actggttatt
    15361 ccttcaaggg gtgagttgtt cttaaggaaa gcataaaaaa aacatgcata caacaatcag
    15421 aacggttctg tctgcttgct tttaatgcca taccaaacgt accattgaga cacttgtttg
    15481 cacagaggat ggcccatgtt cacgggaagt attgtcgcga ttgttactcc gatggatgaa
    15541 aaaggtaatg tctgtcgggc tagcttgaaa aaactgattg attatcatgt cgccagcggt
    15601 acttcggcga tcgtttctgt tggcaccact ggcgagtccg ctaccttaaa tcatgacgaa
    15661 catgctgatg tggtgatgat gacgctggag ctggctgacg ggcgcattcc ggtgattgcc
    15721 gggaccggtg ctaacgctac tgcggaagcc attagcctga cgcagcgctt caatgacagc
    15781 ggtatcgtcg gctgcctgac ggtaacccct tactacaatc gtccgtcgca agaaggtttg
    15841 tatcagcatt tcaaagccat cgctgaacat actgacctgc cgcaaattct gtataatgtg
    15901 ccgtcccgta ctggctgcga tctgctcccg gaaacggtgg gccgtctggc gaaagtaaaa
    15961 aatattatcg gaatcaaaga ggcaacaggg aacttaacgc gtgtaaacca gatcaaagag
    16021 ctggtttcag atgattttgt tctgctgagc ggcgatgatg cgagcgcgct ggacttcatg
    16081 caattaggcg gtcatggggt tatttccgtt acggctaacg tcgcagcgcg tgatatggcc
    16141 cagatgtgca aactggcagc agaagggcat tttgccgagg cacgcgttat taatcagcgt
    16201 ctgatgccat tacacaacaa actatttgtc gaacccaatc caatcccggt gaaatgggca
    16261 tgtaaggaac tgggtcttgt ggcgaccgat acgctgcgcc tgccaatgac accaatcacc
    16321 gacagtggtc gtgagacggt cagagcggcg cttaagcatg ccggtttgct gtaaagttta
    16381 gggagatttg atggcttact ctgttcaaaa gtcgcgcctg gcaaaggttg cgggtgtttc
    16441 gcttgtttta ttactcgctg cctgtagttc tgactcacgc tataagcgtc aggtcagtgg
    16501 tgatgaagcc tacctggaag cggcaccgct tgcggagctt catgccccgg ctggaatgat
    16561 tttgccggtg acctccggcg attatgcaat cccggtgacc aacggtagtg gtgctgtcgg
    16621 taaggcgctg gacattcgtc caccagccca gccgttggca ctggtttctg gcgcgcgtac
    16681 ccagttcacg ggcgataccg cttcattgct ggtggaaaat ggtcgtggca atactctgtg
    16741 gccgcaggtg gttagcgtgc tgcaggcgaa aaactacacc atcacccaac gtgatgatgc
    16801 tggtcagaca ctgaccaccg attgggtaca atggaaccgt ctggacgaag acgagcagta
    16861 tcgtggtcgt tatcaaatct ctgttaagcc gcagggttat cagcaggcgg ttacggttaa
    16921 actgctgaac ctggaacagg cgggcaaacc ggttgcagac gcggcttcca tgcagcgtta
    16981 cagcacggag atgatgaacg ttatttccgc cggtctggat aaatctgcca ctgacgccgc
    17041 gaacgctgcg caaaaccgtg cctccaccac tatggacgta caaagtgcag ctgatgacac
    17101 cggtttacca atgctggtcg tacgcgggcc gttcaatgtg gtttggcaac gtctgccagc
    17161 ggcgctggaa aaagtgggca tgaaagtgac cgacagcacc cgttcgcagg gcaacatggc
    17221 cgtaacttat aagccgctgt ctgacagcga ctggcaggaa ctgggcgcga gcgatccagg
    17281 cctggcatcc ggtgactata aactgcaggt tggcgattta gataaccgca gcagcctaca
    17341 gttcatcgat ccgaaaggtc atactctgac tcagagtcag aacgacgcgc tggtagctgt
    17401 cttccaggct gcgtttagca agtaaaaata cagggctgga gtcatccggc ccttttttct
    17461 gatatgatac gcaaacgtgt gcgtcgacag aaaaacgcga ttttagcggt aattcgcacg
    17521 aaatttgttt gtcggacgta ggtcggataa ggcgttcacg ccgcatccga caaaacatcc
    17581 ggcacaccag acagcaaaag attttaaaac gttaattcac acccaggagt gataaagatg
    17641 caaaagcaag ctgagttgta tcgtggtaaa gcgaaaaccg tatacagcac ggaaaacccg
    17701 gacctgttgg tgctcgaatt ccgcaatgat acgtcagcag gggatggcgc gcgcattgag
    17761 cagtttgatc gcaaaggtat ggtgaacaac aagttcaact acttcattat gagcaaactg
    17821 gctgaagcgg gtatcccgac tcaaatggag cgtctgctct ccgataccga atgtctggtg
    17881 aaaaagctgg atatggtgcc ggttgagtgt gtcgtgcgta accgtgctgc tggctctctg
    17941 gtgaaacgtc ttggaatcga agaaggtatt gagctgaacc cgccgctgtt cgatctgttc
    18001 ctgaaaaacg acgccatgca cgatccgatg gtcaacgaat cttactgcga aacctttggc
    18061 tgggtaagta aagagaacct ggcgcgtatg aaagagctga cctacaaagc gaacgacgtg
    18121 ctgaaaaaac tgttcgatga tgctggtctg attctggtcg acttcaagct ggagtttggt
    18181 ctgtacaaag gtgaagtggt actgggcgat gagttctccc cggacggcag ccgcctgtgg
    18241 gacaaagaaa cgctggagaa aatggacaaa gaccgtttcc gccagagcct cggtggcctg
    18301 atcgaggcct atgaagccgt cgcccgccgc ctgggtgtac agctggactg atttttctgg
    18361 ttgcttgttt atttagccgg agggtactcc ggctaaatgt cttcgatacc cccgctacaa
    18421 tacttcgcat tatctccctt tgacaggaaa gtaataacag gccaatatgt atatacatga
    18481 cgtatatccc aaaaaaaagg gggggctatg gaaactactg tatttttaag taatcgcagt
    18541 caggccgtca gattaccgaa agcggtcgct ctgccagagg acgttaagaa agtagaaatt
    18601 attgcgattg gtcgtacaag aatcatcact cccgccggtg agtcctggga cagttggttt
    18661 gatggcgaaa atgtgagtgc ggattttatg gatatccgtg atcaacccgc aatgcaggaa
    18721 agggaatcat tctgatgctg aaattcatgc tggataccaa catctgtatt ttcactatta
    18781 agaataaacc agtacatgtc agagagcggt ttaacctgaa ttctggccgg atgtgtataa
    18841 gttctgtaac attgatggag cttatctatg gggcagaaaa aagtcagatg ccagaacgca
    18901 atttggcggt gattgaaggt ttcgtatctc gtttggaagt gcttgattat gatagtgctg
    18961 ctgcgacgca tactgggcaa atcagggccg agcttgcccg gcagggaagg cctgttggac
    19021 cttttgacca gatgatagcg ggacatgctc gcagtcgcgg tttgattgtg gtaaccaaca
    19081 atacccgaga gtttgagcga gtggcgggaa tcagactaga agactggagc taacattctc
    19141 ttttacttct aacattcgta aattatataa cgtggcatga agccttaatt cctccgccat
    19201 ttcatcccgc tttcctgtgg taatcttcct ctgaaccacc tacgatcatt tctataatga
    19261 atatattgtt gaggtatcta tgcgttggca agggcgacgt gaaagtgaca atgttgaaga
    19321 caggcgcaac agctctggtg gtccgtctat gggcggtccc ggttttcgcc tgccaagcgg
    19381 taaaggcggg ctgattttac tgatagtcgt gctggttgca ggctactatg gcgttgattt
    19441 aaccgggttg atgaccggac agccggtttc ccaacagcaa tcaacgcggt caattagccc
    19501 taatgaagac gaagccgcaa aattcacctc ggtgattctg gcaaccacgg aagacacctg
    19561 gggacagcaa ttcgagaaga tgggtaaaac ctatcagcaa ccgaagctgg tcatgtaccg
    19621 tggaatgacg cgtaccggct gcggggcggg acagtccatt atggggccat tctattgccc
    19681 ggcggatggc acggtttata tcgatctctc tttctatgat gacatgaaag acaaacttgg
    19741 cgcggatggc gattttgccc aggggtacgt tatcgcccat gaagtcggtc atcatgtgca
    19801 gaaactgtta ggcatcgagc cgaaagttcg tcaactgcaa caaaacgcga cgcaggcgga
    19861 agtgaaccgc ttatctgtgc gtatggaact ccaggccgac tgttttgccg gtgtctgggg
    19921 gcatagtatg cagcagcaag gcgttctgga aaccggcgat ctggaagagg cgctgaatgc
    19981 ggcgcaggcc atcggcgatg accgtttaca acagcaaagt caggggcgag tagtaccaga
    20041 cagtttcact catggtactt ctcagcaacg ctacagctgg tttaaacgtg gtttcgacag
    20101 cggcgatccg gcacaatgca atacttttgg taaaagcatt taacttccgg ggcagggatg
    20161 gctgaactga ctgcgcttca cacattaaca gcgcaaatga aacgtgaagg gatccgccgc
    20221 ttgctggtgt tgagcgggga agagggttgg tgttttgatc atgcgcttaa gttgcgtgat
    20281 gccttacctg gcgactggct gtggatttcg ccgcagccag atgctgaaaa ccactgtttt
    20341 ccctcggcgc tacaaacttt acttgggcgc gagttccggc atgcggtatt cgacgcccgt
    20401 cacggctttg atgctgcttc ctttgccgca cttagcggaa cgttgaaagc gggaagctgg
    20461 ctggtgttgt tactccctat atgggatgag tgggaaaacc aacctgatgc cgactcgctg
    20521 cgctggagtg attgccctga ccctattgcg acgccgcatt ttgtccagca tttcaaacgc
    20581 gtacttacgg cgaataacga cgctatcctc tggcggcaaa accagccgtt ctcgttggcg
    20641 cattttactc cccgtactga ctggcacccc gctaccggcg caccacaacc agaacaacag
    20701 caactcttac agcagctact gaccatgccg ccgggcgtgg cagcggtaac tgctgcgcgt
    20761 gggcgcggta agtcggcctt ggcagggcaa ctcatttctc gtattgcggg cagtgcgatt
    20821 gtcaccgcgc ccgcaaaagc ggcaacggat gtactggcac aatttgcggg cgagaagttt
    20881 cgctttattg caccggatgc cttgttagcc agcgatgagc aagccgattg gctggtggtc
    20941 gatgaagccg cagctatacc tgcgccgttg ttgtatcaac tggtatcgcg ttttcctcga
    21001 acgttgttaa ctactacggt gcagggctac gaaggcaccg gacgtggttt tttgctgaaa
    21061 ttttgcgctc gctttccgca tttacaccgt tttgaactac aacagccgat ccgttgggcg
    21121 cagggatgcc cgctggaaaa aatggttagt gaggcactgg tttttgacga tgaaaacttc
    21181 acccatacac cacaaggcaa tatcgttatt tccgcatttg aacagacgtt atggcgaagc
    21241 gagccagaaa cgccgttaaa ggtttatcag ttattgtctg gtgcgcacta ccggacttcg
    21301 ccgctggatt tacgccggat gatggatgca ccagggcaac attttttaca ggcggctggc
    21361 gaaaacgaga ttgccggggc gctgtggctg gtggatgagg gcggattatc tcaagaactc
    21421 agtcaggcgg tatgggcagg ttttcgtcgc ccgcggggta atctggtggc ccagtcgctg
    21481 gcggcgcacg gcagcaatcc actggcggcg acattgcgcg gaaggcgggt cagccgaata
    21541 gcagttcatc cggctcgtca gcgggaaggc acaggtcagc aactgattgc tggtgctttg
    21601 caatatacgc aggacctcga ctatctttcg gtgagttttg gttacaccgg ggaattatgg
    21661 cgtttctggc aacgctgcgg ttttgtgctg gtgcgaatgg gtaatcatcg tgaagccagc
    21721 agcggttgct atacggcgat ggcactgtta ccgatgagtg atgcgggtaa acagctggct
    21781 gaacgtgagc attaccgttt acgtcgcgat gcgcaagctc tcgcgcagtg gaatggcgaa
    21841 atgcttcctg ttgatccact aaacgatgcc gtcctttctg acgacgactg gcttgaactg
    21901 gccggttttg ctttcactca tcgtccgcta ttaacatcgt taggttgctt attgcgtctg
    21961 ctacaaacca gcgaactggc attaccggcg ctgcgtgggc gtttacagaa aaacgccagc
    22021 gacgcgcagt tatgtaccac acttaaactt tcaggccgta agctgttact ggttcgccag
    22081 cgggaagagg cagcgcaggc gctattcgca cttgatgatg ttcgcactga gcgtctgcgc
    22141 gatcgcataa cgcaatggca attttttcac tgactccttc aattttctcc catggtcatt
    22201 gtgagtcagc ggcgtaaaat taatgacatc agttaaggag ttcgccatga aacatgacca
    22261 ttttgttgtt caaagcccgg ataaaccagc acaacagttg ttactgcttt tccacggtgt
    22321 cggggataac ccggtggcaa tgggggaaat aggcagctgg tttgcgccgc tgtttcctga
    22381 tgcgctggtg gtgagtgtcg gtggcgcgga gccgagcggt aatccggcgg ggcgtcagtg
    22441 gttttcggtg cagggtatta cggaagataa tcgccaggcg cgtgtggatg ccatcatgcc
    22501 gacgtttatt gagacggtgc gctactggca gaaacaaagc ggagtagggg ctaatgccac
    22561 ggcgctaatc ggtttctcac aaggcgcgat tatggcgctg gagagcatta aagccgaacc
    22621 gggccttgct tcgcgcgtca ttgccttcaa cggacgctac gcgagcctgc cggaaaccgc
    22681 gtctacagcc acgacaattc acttgattca tggcggtgaa gatccggtta tcgatctggc
    22741 gcatgcggtt gcggcgcagg aagcgttaat cagtgccggt ggcgatgtga cgctggatat
    22801 cgtggaggat ttaggccatg cgatcgacaa tcgcagtatg caattagccc tggatcactt
    22861 gcgttatacc attccgaaac attatttcga cgaagcgcta agcggcggta agcctggcga
    22921 tgatgatgtg attgaaatga tgtaataata gccgggtagg tctgataaga ctgtatcagg
    22981 catcatggcc ggatgcggcg ttaacgcctt atccggccac ttagcttact tcttcggttg
    23041 atcctttttc ggccagtcgt cgtcatcgtc ccacttatcg ttgaaatcac gatgcggggg
    23101 aagctccggc ttgttggcga gaaatttctt atggtcgacg cgcttgagat ctttaatcac
    23161 gttcagcagg acgcctacca aaaagacaat caccagaatc caccaatatt tagccagcca
    23221 gtccatgctt atttcctctt gcagaaccac tcatcaggcg acgagctgtt ccatgatacg
    23281 ttgatacata cgggcaagta gctgcaggtc ggcagcgttc acacattcat taattttatg
    23341 aatcgtggca ttgaccggcc cgagttccac cacctgcgcc cccatgcggg caataaagcg
    23401 cccgtcggac gttccgcctg tggtcagtag ctgcggttta atttcattat agtgctcaac
    23461 cgcgttaacg accgcatcca ccagtttacc gcgcgcggtc aaaaatggct gcccggaaag
    23521 ccaccaatcc accgtatagc gcagttgatg tttttcaagc agggcaagta cctgcgcttt
    23581 gatcatctca tccgtcagtt cggtgctgaa gcggaagtta aactgcacaa acagttcacc
    23641 cggaataacg ttgttactgc ccgttcccgc ctgaatattg gcaatctgca tactggtcgc
    23701 cgggaagaat tcattgccct gatcccactc aatagccact aattcattaa ggaaaggtgc
    23761 tgcgcgatgt accggattgt cagccagatg tgggtaggca acatgcccct gcacgccatg
    23821 aatagtaagg ttacaggtta acgatccgcg acgaccattt ttcaccacat caccaaccac
    23881 ttcgatactc gacggttcgc cgaccaggca gtaatcgaga cgctcattac gcgccattaa
    23941 cgcttcgacg acttttaccg taccgttgtg ggcactggct tcttcatcag aggtgatcag
    24001 aaatgccagt cgtcccgtat ggttgggatg ttgtgcgaca aagcgttctg acgctaccac
    24061 catcgccgcc agcgagcctt tcatatctgc cgcaccgcgc ccgaataaca tgccatcacg
    24121 aatggtgggt tcaaacggcg gattgatcca acggtcggcg tcgccaggcg gcaccacgtc
    24181 ggtatgcccg gcaaaggcta acgtttcacc ctgcccacgc catgcccaaa aattctgcgt
    24241 gtcggcaaag tccatgcgtt caacggtaaa accgatcgcc tgcaaacgtt caatcaacaa
    24301 agcctggcat cctgcgtcat caggactcag ggaagggcgg cgaataagct gttgtgtcag
    24361 ctcaataacc gggcacgaca tagactacac ctcatggaaa aattgctgat aactggattc
    24421 actgaaaccc agcagcatag gcttaccagg cgcgcagagc aatggacgtt tgataattgc
    24481 aggcatttca gtcattaatg tcgccgcaga ggccgcatcg gtgattttat tgcgggtggt
    24541 ttcgtccagt ttacgccagg ttgtaccacg ggtgttgagt aacgcttccc agcctaattc
    24601 gttgataaaa ccgttcaata attcgctgtc caatccatcg acgcggtaat catgaaaacg
    24661 atagtcgatg ttattagctt ccagccaacg gcgagccttc ttaatggtgt cacaattttt
    24721 gatgccgtaa agtgtaacca taatgaatcc tttttcgatt tatgagaata aatactcatt
    24781 taaaggtgaa aacattttgc ctgcaaatga ttatcaggaa atataacatt atcgaagtat
    24841 tcaaattaac aaccattaaa taa
//
LOCUS       DAPHCV010000076        24639 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.076, whole genome shotgun sequence.
ACCESSION   DAPHCV010000076 DAPHCV010000000
VERSION     DAPHCV010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.076"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            46..975
                     /gene="pfkB"
                     /locus_tag="RH033_003051"
     CDS             46..975
                     /gene="pfkB"
                     /locus_tag="RH033_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HDV2992203.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVNAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            1076..1366
                     /gene="ghoS"
                     /locus_tag="RH033_003052"
     CDS             1076..1366
                     /gene="ghoS"
                     /locus_tag="RH033_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HDV2992204.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDDG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEIAITTWEEWNSNRK"
     gene            1472..2332
                     /locus_tag="RH033_003053"
     CDS             1472..2332
                     /locus_tag="RH033_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDV2992205.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(2373..2909)
                     /locus_tag="RH033_003054"
     CDS             complement(2373..2909)
                     /locus_tag="RH033_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HDV2992206.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFFLIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            3056..3724
                     /gene="hxpB"
                     /locus_tag="RH033_003055"
     CDS             3056..3724
                     /gene="hxpB"
                     /locus_tag="RH033_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HDV2992207.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVEQVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3888..4478
                     /locus_tag="RH033_003056"
     CDS             3888..4478
                     /locus_tag="RH033_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDV2992208.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            4611..6002
                     /gene="tcyP"
                     /locus_tag="RH033_003057"
     CDS             4611..6002
                     /gene="tcyP"
                     /locus_tag="RH033_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HDV2992209.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGLLLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPMDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(6049..6312)
                     /gene="cedA"
                     /locus_tag="RH033_003058"
     CDS             complement(6049..6312)
                     /gene="cedA"
                     /locus_tag="RH033_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HDV2992210.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQENEVEE"
     gene            6495..8756
                     /gene="katE"
                     /locus_tag="RH033_003059"
     CDS             6495..8756
                     /gene="katE"
                     /locus_tag="RH033_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HDV2992211.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVIWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDLPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKARIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(9014..9772)
                     /gene="chbG"
                     /locus_tag="RH033_003060"
     CDS             complement(9014..9772)
                     /gene="chbG"
                     /locus_tag="RH033_003060"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HDV2992212.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSLDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVAKFAAEEGIALRIDRQPLSNDG
                     DLPANLRSSQGFSSAFYGEEISETLFLQVLDDSSHRGERSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGCYRDV"
     gene            complement(9785..11137)
                     /gene="celF"
                     /locus_tag="RH033_003061"
                     /gene_synonym="chbF"
     CDS             complement(9785..11137)
                     /gene="celF"
                     /locus_tag="RH033_003061"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDV2992213.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSMNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(11241..12083)
                     /gene="chbR"
                     /locus_tag="RH033_003062"
     CDS             complement(11241..12083)
                     /gene="chbR"
                     /locus_tag="RH033_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HDV2992214.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDIPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(12091..12441)
                     /gene="chbA"
                     /locus_tag="RH033_003063"
     CDS             complement(12091..12441)
                     /gene="chbA"
                     /locus_tag="RH033_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HDV2992215.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(12492..13850)
                     /gene="chbC"
                     /locus_tag="RH033_003064"
     CDS             complement(12492..13850)
                     /gene="chbC"
                     /locus_tag="RH033_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HDV2992216.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKIDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVHRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(13935..14255)
                     /gene="chbB"
                     /locus_tag="RH033_003065"
     CDS             complement(13935..14255)
                     /gene="chbB"
                     /locus_tag="RH033_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HDV2992217.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(14554..14892)
                     /gene="osmE"
                     /locus_tag="RH033_003066"
     CDS             complement(14554..14892)
                     /gene="osmE"
                     /locus_tag="RH033_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HDV2992218.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQATK"
     gene            15094..15921
                     /gene="nadE"
                     /locus_tag="RH033_003067"
     CDS             15094..15921
                     /gene="nadE"
                     /locus_tag="RH033_003067"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDV2992219.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            16151..17038
                     /gene="cho"
                     /locus_tag="RH033_003068"
     CDS             16151..17038
                     /gene="cho"
                     /locus_tag="RH033_003068"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HDV2992220.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWNGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRAPAGFDHDGYKILCKPL
                     LSGNYEITELDPVNNQQAS"
     gene            complement(16998..17573)
                     /gene="ves"
                     /locus_tag="RH033_003069"
     CDS             complement(16998..17573)
                     /gene="ves"
                     /locus_tag="RH033_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HDV2992221.1"
                     /translation="MEYFDMRKLSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGYSPDQVQ"
     gene            complement(17776..18261)
                     /gene="spy"
                     /locus_tag="RH033_003070"
     CDS             complement(17776..18261)
                     /gene="spy"
                     /locus_tag="RH033_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HDV2992222.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTSAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(18591..19559)
                     /gene="astE"
                     /locus_tag="RH033_003071"
     CDS             complement(18591..19559)
                     /gene="astE"
                     /locus_tag="RH033_003071"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HDV2992223.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(19552..20895)
                     /gene="astB"
                     /locus_tag="RH033_003072"
     CDS             complement(19552..20895)
                     /gene="astB"
                     /locus_tag="RH033_003072"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HDV2992224.1"
                     /translation="MNAWEINFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAS
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECRKHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(20892..22370)
                     /gene="astD"
                     /locus_tag="RH033_003073"
     CDS             complement(20892..22370)
                     /gene="astD"
                     /locus_tag="RH033_003073"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HDV2992225.1"
                     /translation="MTLWINGDWVTGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSFGDRQVRVERFAGLLESNKAELTAIIARETGKPRWEAATEVMAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGTQGDAFLARLVAVSQRLTPGRWDDEPQPFIGGLISEQAAQQVVTAW
                     QELEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVTGVPDEEVFGPLLRVWRYDNFDEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(22367..23401)
                     /gene="astA"
                     /locus_tag="RH033_003074"
     CDS             complement(22367..23401)
                     /gene="astA"
                     /locus_tag="RH033_003074"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HDV2992226.1"
                     /translation="MMVIRPVERTDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPEWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGEFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(23398..24618)
                     /gene="astC"
                     /locus_tag="RH033_003075"
     CDS             complement(23398..24618)
                     /gene="astC"
                     /locus_tag="RH033_003075"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HDV2992227.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFAYRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLDLINTPEMLNGVKQRHDWFVERLNTINHRCGLFSEIRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAVACERFVSGGSS"
BASE COUNT         6146 a         6513 c         5952 g         6028 t
ORIGIN      
        1 ccgctgattc ggtgccagac tgaaatcagc ctataggagg aaatgatggt acgtatctat
       61 acgttgacac ttgcgccctc tctcgatagc gcaacaatta ccccgcaaat ttatcccgaa
      121 ggaaagctgc gctgtaccgc accggtattc gaacccggag gcggcggcat caacgtcgcc
      181 cgcgccattg cccatcttgg aggcagtgcc acagcgatct tcccggcggg tggtgcgacc
      241 ggcgaacatc tggtttcact gttggcagat gaaaatgtcc ccgtcgctac tgtaaacgcc
      301 aaagactgga cccggcagaa tttacacgta catgtggaag caagcggtga gcagtatcgt
      361 tttgttatgc caggcgcggc attaaatgaa gatgagtttc gccagcttga agagcaagtt
      421 ctggaaattg aatccggggc catcctggtc ataagcggaa gcctgccgcc aggtgtgaag
      481 ctggaaaaat taacccaact gatttccgct gcgcaaaacc aagggatccg ctgtatcgtc
      541 gacagttctg gcgaagcgtt aagtgcagca ctggcgattg gtaacatcga gttggttaag
      601 cctaaccaaa aagaactcag tgcgctggtg aatcgcgaac tcacccagcc agacgatgtc
      661 cgcaaagccg cgcaggaaat cgttaatagc ggcaaggcca aacgagttgt cgtttccctg
      721 ggtccacaag gggcgctggg cgttgatagt gaaaactgca ttcaggttgt gccaccaccg
      781 gtgaaaagcc agagcaccgt tggcgctggt gacagcatgg tcggcgcgat gacactgaaa
      841 ctggcagaaa atgcctctct tgaagagatg gtccgttttg gcgtagctgc ggggagtgca
      901 gccacactca atcagggaac acgcctgtgc tcccatgacg atacacaaaa aatttacgct
      961 tacctttccc gctaactgaa acattccccc agcattgggg gaatcaccac caacctgtcg
     1021 gcaacgcgtt tctccgacta tgctcaaaag tcatgtgata acaaaggggt gaactatggc
     1081 cagtggcgat cttgtccgtt atgtcataac cgtcatgttg catgaggata cattgactga
     1141 aattaacgag ttgaataatt acctgactcg cgacggtttt ttgctaacca tgacggatga
     1201 tgatggaaat atccatgagc tggggactaa tacttttgga cttatcagta cccaaagtga
     1261 agaagaaatt agagaacttg tttcggggct tacccaaagt gcaaccggta aagatcctga
     1321 aatagccatc acgacctggg aggaatggaa tagcaacaga aaataaatgg tttttgggca
     1381 ataatcagtc tgtggtgtgc gttagctcgt gtttttacac cgcattcttg cgctaacctt
     1441 atgatctggc agacaacatg ggagagacat catgtggcag gcaatcagtc gtcttttgag
     1501 cgagcagtta ggtgaaggcg aaatcgaact gcgtaatgaa ctgcctggcg gagaagtcca
     1561 tgccgcatgg catttgcgct atgcaggaca tgactttttc gtcaaatgtg atgaaaggga
     1621 actgcttccc ggttttaccg ccgaagccga ccaactggag ttactgtcgc gtagtaaaac
     1681 cgtcaccgtg cctaaggttt gggcagttgg cgctgaccgt gactacagtt ttctggtgat
     1741 ggattatctc ccacctcgcc cgctggatgc gcatagcgca tttattcttg gtcagcaaat
     1801 tgcacgttta catcaatgga gcgaccaacc gcaatttggc ctcgatttcg ataacgcgct
     1861 ctccacaact ccccagccca acacctggca acgtcgctgg tcaacttttt ttgctgaaca
     1921 acggattggc tggcagctgg aactggcggc agaaaaaggg atcgctttcg gcaatatcga
     1981 tgccatcgtc gagcatattc agcagcgtct ggcctcgcat caaccgcagc cttctctgtt
     2041 gcacggcgat ttatggtccg gcaactgtgc gctgggtccg gatggcccgt acattttcga
     2101 cccggcctgc tactggggtg accgagagtg cgacctggcg atgttaccgc tgcatactga
     2161 acagccgcca caaatctatg acggctatca gtcagtatcc ccgctacctg ccgatttcct
     2221 tgaacgtcaa ccggtttacc aactctacac gctgttaaat cgtgcaaggt tatttggcgg
     2281 tcagcatttg gttattgctc agcagtcact ggatagatta ttagcagcat aatatggggt
     2341 gaggataatg gccgctccgt gcggcctttt gattaaataa accccagcag agaaaagaac
     2401 acataacctg cagcaataca aataaccggc aggatataca gcgagaaaaa ctgcaggaaa
     2461 atagtatggt gcggcacaac aatacgtgac tcaatttgtt cacgcgttag cccctcttcc
     2521 cctttggctt tttccagaat gagttgatct tcaacacctt cccgcaagaa gcgcgcctgg
     2581 cgactcatcc gggcaccgga atcctgcaac gccagtccga caaaaatcag gaagaaaatc
     2641 acccagaaca taacgttcac gccaccatta aaattgggcg tcggggagtt ataccagaac
     2701 agattcaaaa aaggcgtatt cgcctgcatc atatcgatca tgacatgagt aaagtcgagc
     2761 atgaccgcat taatgccttc ctggttttcc tgccgggtat tcataaattt aagcaaagaa
     2821 atcaatgtag agatcaatgc aggtataaaa atcacccaac ccaaaatcct tttcaaaaca
     2881 gcaatgcgtc cagcttgttg atacgtcatg agttctcctt gattaagacg cgtcgtttca
     2941 cttagtttac ctgtagatat ctgttttcgc ccattcttta aaggcgatat gataggcgct
     3001 taatcataag cacggcttaa taccttacat ataatgctct aaaggagagg ttgtaatgtc
     3061 aaccccgcgt cagattcttg ctgcaatttt tgatatggat ggattactta tcgactcaga
     3121 acctttatgg gatcgagccg aactggatgt gatggcaagc ctgggggtgg atatctcccg
     3181 tcgtaacgag ctgccggaca ccttaggttt acgcatcgat atggtggtcg atctttggta
     3241 cgcccggcaa ccatggaatg ggccaagccg tcaggaagta gtagaacaag ttattgctcg
     3301 tgccatttca ctggttgaag agacgcgtcc attattacca ggtgtgcgtg aagccgttgc
     3361 gttatgcaaa gaacaaggtt tattggtggg gctggcctcc gcgtcaccgc tgcatatgct
     3421 ggaaaaagta ttgaccatgt ttgatttacg cgacagtttc gatgccctcg cctcggctga
     3481 aaaattgcct tacagcaagc cgcatccgca agtatatctc gactgcgcag caaaactggg
     3541 ggttgaccct ctgacctgcg tggcactgga agattcggta aatggcatga tcgcctctaa
     3601 agcagcccgc atgcgttcca tcgtcgttcc tgcgccagaa gcgcaaaatg atccacgttt
     3661 tgtattagca aacgtcaaac tctcatcgct gacagaactc accgcaaaag accttctcgg
     3721 ttaatgacca gggggcagtg atcacactgc ccctggttct ttatctgaat ttgcattcaa
     3781 ctgacggatt aatcgccaat ttaagagaaa gagttacacc gtcaccactt ccgtgcactg
     3841 tataaaaacc ctatactgta cgtatcgaca gtttagtgag ttttatcatg acggcggaag
     3901 gtcaccttct cttttctatt gcttgtgcgg tatttgccaa aaatgccgag ctgacgcccg
     3961 tgctggcaca gggtgactgg tggcatattg tcccttccgc aatcctgacg tgtttgttac
     4021 cggacatcga tcacccaaag tcgtttcttg ggcagcgatt aaagtggata tcaaaaccaa
     4081 tcgcccgcgc ttttgggcat cgtggtttta cccacagtct gctggcggta tttgcgctgc
     4141 tggcaacctt ttaccttaag gttcctgaaa gctggttcat tccggctgat gcgctacaag
     4201 gaatggtgct gggttatttg agccacatac ttgccgatat gctgacaccc gccggtgttc
     4261 ccctgctctg gccatgccgc tggcgtttcc gcttgcctat cctggttccc caaaagggca
     4321 accaactgga acgttttatc tgcatggcat tatttgtctg gtcggtatgg atgccccatt
     4381 cattacccga aaacagcgct gtccgttggt catcgcaaat gatcaatacc ttgcagatcc
     4441 agtttcatcg gcttattaag catcaggttg aatactaaaa aggcaaaaat cacctttctg
     4501 gaataagcaa ttccatttga atataagagc cagctcacag ttctgttaat cttgcgccaa
     4561 cactatgact gctacgcagt gatagaaata ataagatcag gagaacgggg atgaactttc
     4621 cattaattgc gaacatcgtg gtgttcgttg tactgctgtt tgcgctggct cagacccgcc
     4681 ataaacagtg gagtctggcg aaaaaagtgc tggtgggtct ggtgatgggt gtggtttttg
     4741 gcctcgccct gcataccatt tatggttctg acagccaggt acttaaagat tctgtacagt
     4801 ggtttaacat tgttggtaac ggctatgttc aactcctgca aatgatcgtt atgccgttag
     4861 tctttgcctc tattctgagc gcggttgccc gtctgcataa cgcatcacag ttaggcaaaa
     4921 tcagttttct gaccatcgga acgcttttgt ttaccacact gattgcggcg ctggtcggtg
     4981 tgctggtcac caacctgttt ggtttgacgg ctgaaggtct ggttcagggt ggtgcagaaa
     5041 ctgcacgttt gaacgccatc gaaagtaact atgttggtaa agtctctgac ctgagcgttc
     5101 cgcagctggt cttgtccttt atcccgaaaa acccgtttgc cgatttgacc ggagccaatc
     5161 cgacgtcaat tatcagcgtg gtaatttttg ccgcattcct cggcgtagct gcgctgaagc
     5221 tgctgaagga tgatgcgccg aaaggtgaac gcgtcttaac cgctatcgat accctgcaaa
     5281 gctgggtgat gaaactggtt cgcctggtca tgcagctgac cccttacggc gttctggcgc
     5341 tgatgaccaa agtggttgca ggttctaacc tgcaagacat catcaaactg ggaagtttcg
     5401 ttgtcgcgtc ctacctcggt cttctgatta tgtttgcagt gcatggcctt ctgctgggta
     5461 ttaatggcgt gagcccgctg aagtacttcc gtaaggtatg gccagtactg acgtttgcct
     5521 ttaccagccg ttccagtgct gcgtctatcc cactgaatgt ggaagcacaa acgcgtcgac
     5581 tcggtgttcc tgaatccatc gccagtttcg ccgcctcttt cggtgcaacc attggtcaga
     5641 acggctgtgc cggtttgtat ccggcgatgc tggcggtgat ggttgcgcct acggttggca
     5701 ttaacccgat ggacccgatg tggattgcga cgctggtcgg tattgttacc gttagttccg
     5761 caggcgttgc cggtgtcggt ggtggtgcaa ctttcgccgc actgattgtg ctgcctgcga
     5821 tgggcctgcc ggtaacgctg gtggcattgt taatctccgt tgaaccgctt atcgacatgg
     5881 gtcgtacggc gctaaacgtt agtggctcga tgaccgctgg cacgctgacc agccagtggc
     5941 tgaagcaaac cgataaagct attctggata gcgaagacga cgccgaactg gcacaccgtt
     6001 aatcctgatt tgttacgtta aacgcctgtc atcccgacag gtgttttttt attcttcgac
     6061 ctcattttcc tgacgaatct gatttgccca acgctgggcg gattcaggca cagtaaacgc
     6121 cggtgagcgc agaaatgact ctcccatcag tacaaacgca acatatttgc cacgcagcat
     6181 ccagacatca cgaaacgaat ccatctttat cgcatgttct ggcggcgcgg gttccgtgcg
     6241 tgggacatag ctaataatct ggcggttttg ctgacggagc ggtttcttca ttactggctt
     6301 cactaaacgc atattaaaaa tcagaaaaac tgtagtttag ccgatttagc ccctgtacgt
     6361 cccgctttgc gtgtatttca taacaccgtt tccagaatag tttccgaagc gggatctggc
     6421 tggtggtcta tagttagagg gttttttgac caaaacagcg gccctttcag taataattta
     6481 aggagacgag ttcaatgtcg caacataacg aaaagaaccc acatcagcac cagtcaccac
     6541 tacacgattc cagcgaagcg aaaccgggga tggactcact ggcacctgaa gacggatctc
     6601 atcgtccagc ggctgaacca acaccgcctg gtgcacaacc taccgcccca gggagcctga
     6661 aagcccctga tacgcgtaac gaaaaactta attctctgga agacgtacgc aaaggcagtg
     6721 aaaattatgc gctgaccact aatcagggcg tgcgcatcgc cgacgatcaa aactcactgc
     6781 gtgccggtag ccgtggtcca actctgctgg aagattttat tctgcgcgag aaaatcactc
     6841 actttgacca tgagcgcatc ccggaacgta ttgttcatgc acgcggatca gcagctcacg
     6901 gttatttcca gccatataaa agcttaagcg atatcaccaa agcagatttc ctctcagatc
     6961 cgaacaaaat caccccagta tttgtacgtt tctctaccgt tcagggtggt gctggctctg
     7021 ccgataccgt gcgtgatatc cgtggctttg ccaccaagtt ctataccgaa gagggtattt
     7081 ttgaccttgt tggcaataac acgccaatct tctttatcca ggatgcgcat aaattccctg
     7141 attttgttca tgcggtaaaa ccagaaccac actgggcaat tccacaaggg caaagcgccc
     7201 acgatacttt ctgggattac gtttctctgc aacctgaaac tctgcacaac gtgatatggg
     7261 cgatgtcgga tcgcggtatc ccgcgcagtt accgcaccat ggaaggcttc ggtattcaca
     7321 ccttccgcct gattaatgcc gaagggaagg caacgttcgt acgtttccac tggaaaccac
     7381 tggcaggtaa agcctcactc gtttgggatg aagcacaaaa actaaccgga cgtgacccgg
     7441 acttccaccg ccgcgagttg tgggaagcca tcgaagcagg cgatttaccg gaatacgaac
     7501 tgggcttcca gttgattcct gaggaagatg aatttaagtt cgacttcgac cttcttgatc
     7561 caactaaact tatcccggaa gaactggtgc ccgttcagcg tgtcggcaag atggtgctca
     7621 atcgcaatcc ggataacttc tttgccgaaa acgaacaggc ggctttccat cctgggcata
     7681 ttgtccccgg tctggatttc accaacgatc cgctgttgca ggggcgtttg ttctcttata
     7741 ccgatacaca aatcagtcgt cttggcggac caaatttcca tgagattccg attaaccgcc
     7801 cgacctgccc ttaccataat ttccagcgtg acggcatgca tcgtatgggg atcgacacta
     7861 acccggcgaa ttacgaaccg aactcgatca acgataactg gccgcgcgaa acaccgccgg
     7921 ggccgaaacg cggcggtttt gaatcatacc aggagcgcgt ggaaggcaat aaagttcgcg
     7981 agcgcagccc atcgtttggc gaatattatt cccatccgcg tctgttctgg ctaagtcaga
     8041 cgccattcga gcagcgccat attgtcgatg gtttcagttt tgagttaagc aaagtagttc
     8101 gtccgtatat tcgtgagcgc gttgttgacc agctggcgca tattgatctc actctggccc
     8161 aggcggtggc gaaaaatctc ggtatcgaac tgactgacga ccagctgaat atcaccccgc
     8221 ctccggacgt caacggtctg aaaaaggatc catccttaag tttgtacgcc attcctgacg
     8281 gtgatgtgaa aggtcgcgtg gtagcgattt tacttaatga tgaagtgaga tcggcagacc
     8341 ttctggccat tctcaaggcg ctgaaggcca aaggcgttca tgccaaattg ctctactccc
     8401 gaatgggtga agtgactgcg gatgacggta cggtgttgcc tatagccgct acctttgccg
     8461 gtgcaccttc gctgacggtc gatgcggtca ttgtcccttg cggcaatatc gcggatatcg
     8521 ctgacaacgg cgatgccaac tactacctga tggaagccta caaacacctt aaaccgattg
     8581 cgctggcagg agacgcgcgc aagtttaaag caagaatcaa ggtcgctgat cagggtgaag
     8641 aagggattgt ggaagctgac agcgccgatg gtagttttat ggatgaactg ttaacgctga
     8701 tggcagcaca ccgcgtgtgg tcacgcattc ctaagattga caaaattcct gcctgatggg
     8761 aggcggcgca gttgcgccgc ctcaaatgat ttacatagtg cgctttgttt atgtcggatg
     8821 cggcgtgaac gccttatccg gcctacaaaa ctgtgcaaat tcaatatatt gcaggaaaaa
     8881 cgtaggcctg ataagcgaag cgcatcaggc agttttgcgt ttgtcagcag tctcaagcgg
     8941 cgcagttacg ccgcctttgt aggaattaat cgccggatgc aaggttcacg ccgcatctgg
     9001 caaacatcct cacttacaca tcccgataac accccaaccg ataaccacgc tgagcgatag
     9061 cacctttcaa cgacgctgat gtcaacacat ccagctccgt taaccgcggg aagcaataag
     9121 cactctggcg aatcgtatta tcgataaacg ccgggtggca catcacttcc agagagcgct
     9181 cgccgcgatg actggagtca tcaagcactt gtaggaacag tgtctcgcta atctcttcac
     9241 cataaaacgc gctactgaat ccctgcgaac tgcgcagatt agccggtaaa tcaccatcgt
     9301 tagatagcgg ctgacggtcg atgcgcaacg caatcccctc ttcagccgca aactttgcca
     9361 caatcgggaa aatctgcggg aacatatgca catgatgatg gctatcaaga tgcgtaggtt
     9421 tgcgtccaaa taactcaatg aaacgcaaat actgactggc aagctcctga gtaatttctt
     9481 ccagcggtaa agcatcttct tctgccaact gccagatcca ttttcccagc acaccatcgc
     9541 gagttaaccc cggcatagct gtcagtggct tgcccatagt aaggacaaag tgcatcccta
     9601 tggccagact cggttcatca agactcaact gcaccgcatg gtcaatagcc tgcccattca
     9661 ccagcgccgt cgtcgatgtg acaatcccat tgcgacaggc ctcgataatg ccgtagttct
     9721 gacctttgct taagccaaaa tcatcggcat taacaatcag taagcgttcc ataatcagcc
     9781 tcggttaatg tgctttttta agctctgcga tgcagtcggc aaaatttggc aaccatttct
     9841 cgtgcgccag aatcatctcg cgtgccagca gctcagcatc gcgatcggaa tgcaccaacg
     9901 gactaaggtt tagcgccagt aacacatcgt taaactctcc gctaagtgcg gcgttgctgg
     9961 cagcaatctc gaagccttta atggtgtgaa tcagccccat cactttatca tcgaaatgcg
    10021 taatgcgcgg atgtggcgtc gcgccatcgc gccccagcgt acaggtcatt tctaccgccc
    10081 agtctgccgg aatattatca atatgcccat gatgcgggat attaacgtaa tgttcagctt
    10141 gcttgtcgtt gtagatagcg ttgatcactt cgcacgctgc atcagagtaa taagccccac
    10201 cgcgctgttc cagttctttc ggcttaactt tcaactccgg gtttttatat agctcaaaaa
    10261 gttgtttctc gactttctgt actacctgcg ctcgtgcgcc gcctttgtag tattcgccca
    10321 tttcaatagc cagcatctct ttttgcttga agtaataaag caaataagaa cacggcaaca
    10381 gattcataga acgaattaag ccttcgctaa atggcagatc gaaaatattt ttaacgccag
    10441 atgcttttaa ctgcccggat gccacaccat caagcaattc ggcaaagcgt gatttgccat
    10501 ttaccagcac atccttaatg aacaccatat ggttgaggcc gaacagatcg atagataaat
    10561 catcgctgtc tttcagcatc agaacatcgc gaataaacat cttcatgccg atcggaatat
    10621 tacacacgcc gataaagcgt ttaaatccgg tatgacgata aacggcttca gtgaccattc
    10681 ccgccgggtt agtgaagtta atcacccatg cattcggaca tagttcttcg acatctttta
    10741 cgatgtcaaa aatcaccgga atggtacgca gacctttaaa cagaccgccc gcaccgttag
    10801 tttcctgacc aagataacca tgacttaatg gaatacgttc atccagttcg cgcgccggta
    10861 attggccaac gcgcagttgg gtagtaacga aatcagcatc tttcaatgct tcgcggcgat
    10921 ccagcgtttt ataaagcttc atcgggacgc cagcgttatc aatcatccgt tggcagagat
    10981 caaaaataat atccagtttc gctttaccac cttcgacatc caccagccat aattcgctga
    11041 ccggcaattc gtgataacgc ttaataaatc cttccagtaa ctccggggta tagctgctcc
    11101 cgccaccaat agtgacgact tttaatttct ggctcataat ttctcccttc agtacagaat
    11161 actgatatct ggcatatctg ccccccggac ataaataatc cagcaacagg acagatatgt
    11221 gaattgtcag gtataacgac ttactgatta aattcagtca atttcttacg atagctctta
    11281 ggcgtaaagg atgttaattt cttaaacgtt ttaataaaca agctcgggct actataaccg
    11341 gcctcaaacg caatatccgt cactgaatag ttggtcattt ccagttgttt tttggcaaaa
    11401 ttaatacgga tttcattaat aatctgcatt ggcgttttac catagtatcg ttgagtcgct
    11461 cgcgtcaaat attcctgtga tttggctgac aacgtcacca tattctccag cgccgattca
    11521 ctaaactgct ctttatcatg catcttttct accgtacttt tcagccactg cggtatatca
    11581 tcaatcacct gttcttcgcg gtaatgacgt aaacggttaa tgacataaaa agtaaccatc
    11641 tcaacaaact cttccagccc tgtttcgcgg aaattcaatg aagaaatcac tgtttccaca
    11701 taggtgagaa aagcgttatt ggtccggtat acctgcgaag cgacaaagca ataaggcaac
    11761 aacggcaggt aatgctgctc aaaaaagcgt ttactgatcc caacgttcaa tatacgcgtg
    11821 gcaccaaatt cataaaaact ttgatggtgc gaacctaacg gaataaaaac aaaatcgccc
    11881 cgttccagta acacgcgctt accgttaatc tcctggaaat aacgcccggt taataccaga
    11941 gtaaattcat aatagtcgtg ctgatgcagt ccgctgatac tctcagtttt attgtagata
    12001 aacacatgga aatttttgcc attaaacaac tgctgttctc gggcagtggc aatttccggc
    12061 gcgttaatca ctggctgcat catcgactcc ttatgccttc agtttttcat gaagctcaat
    12121 taattcagta atcagttcac gcgcaagcat ggacgtcatt aaatgatcct gagcgtggac
    12181 cagcaccaga ctcactttca tcttaccttc acccgcatcg ccttcaatca gtttcgtctg
    12241 taccagatgc gcttcattca atgccatccg tgactgatcc atcatggctt ttgctgcggc
    12301 aaaatcgccc tgcttcgcct gtttcagtgc cgcatacgcc aggctgcgcg cttgtccgga
    12361 gttgatgata agccccatca ccacttcttc cagctcttca gcttccgttt gcgtatcggg
    12421 aatgttatcg agatccatca tacatcgttc ctctcttttc ttaccggcac gattacccgt
    12481 accggcatcg attaaaattt cagtgcgtta gcgatatctt cttcgctctc ttctttatca
    12541 atcgcatttt gtgctttgtt agcgaccaca acaaagggca gataaattaa cgttgcgatg
    12601 ccaaggttga agagtgcgac cagcaatgcg gcgacgctac cgttggtatt aaagaaggct
    12661 cccagaccgg ttggcatggt ccacggtgca atattggtca ccggaggaat aatgcccatg
    12721 tagtacgctg cgagggtgat tgccgccaga atcggttgta ccagtacaaa cgggataaac
    12781 atcaccgggt tcatgataat tggcagacca aacagaatcg gttcgttaat ctggaagatg
    12841 ccggacggca gcgccagttt tgccacctga cgataatcag cacggcgaga ggcgataaag
    12901 atagccagga tcaggcctaa agtcgcacca ctgccaccga ggaaaataaa ggagtccagc
    12961 atcggcttgg cccagatatg gaaggtctta ccggctgcca gcgccgcttc gacggaacca
    13021 tattgctgat aggtcgcgat attttccagc gcccaaggcg tcataatgcc gttgtccagt
    13081 gcggtcagcg ccagcgcacc atgaataccg aagaaccaga gcagtggaac aaagatcaca
    13141 taggcccagc ccaccacgct acccaacgat gccagtgggg ttgagatggt atccataatg
    13201 atctgatgga agttggtgcc ccaggtattc aacgcccagg caataatccc catcacggaa
    13261 agaataataa agccggggat taatgccgag aaggaacgcg atactgaagc aggtacgctg
    13321 tcgggcaatt taatgaccca attgcggtgg acaataaagg taaacatttc tgccaccacc
    13381 aggccaataa taatcccgga gatgatattc gccccaccta accagtttgc gccaaccgca
    13441 taggcctcac cgacactata tggggtgacg gtcataaatg ctgcaacgga taacaacccg
    13501 gccgccagcg catcgatttt acgctcttct gccagcgcca tgccaataaa gaacggtgcc
    13561 attaaagaca ttatccctaa tgttccgtta tatacgttgc cgccaatacc tttcagacca
    13621 ttaagtgttt caatggttga ggcatccagg cgaataccta aggaataaaa aaacgacccc
    13681 tccccaaagc ttagaaaaac gttgttaatt aatacaaaca tggcccccgc aagggttaac
    13741 ggcattaagc gaataaagcc atttttgatt gcattaacgt gtggctgctt tcctatttta
    13801 actgcaaaag ggaggagtac cttttcaagc gatgcaataa cattactcat agaaaaatac
    13861 ccttaaaaac cgcaatttaa atattgcggt attgatttat gaaataactc tttgacggga
    13921 aaatttaaaa taaattaatt tgctgcggct tttttaatcg ctgcaaccgc agccttaagc
    13981 acgcctaaac catcgacttt gccataaagc agcgagtcaa ttacttcaac cggtttgttg
    14041 ggtaacaaac gctggatttc gggcaacata taagcaatct gcggccctaa taacacgaca
    14101 tcggcattct gacctttttc accagccagt gtttccggaa atgcttcaat aatgaccgga
    14161 acttcatatt tttctgcctg tgcgcgcatt tttgatacca gtaaagaggt agacatgccc
    14221 gcagaacaaa acagataaat gtgtttcttt tccataaaac tgccctcatc gacgattatc
    14281 tgtcagccag acactccgca agccttaacc tgcttccatg ctctgggtaa cttgcgaaac
    14341 caacatgact ttttttgtgg ctgaaaggag tatacggtat aggcctgagc aatagtattt
    14401 ctttgacctg ataaattgtc tctccttgac ctgtggttat gaccctcttc acatttcggt
    14461 caaataattc gcgaagataa ttaagaactc aggccaaaaa aaaccggcgc aatggccggt
    14521 ttccgttgtt actcaagctt tcaggcgaag tgattacttc gtagcctgtg gatcagtgtc
    14581 gtattcagca caggtctgat aaccggagtt gatgacatgt ccggtatcat ctaacgcgac
    14641 aaagtaggtt tctgctttac catcacgttg acccaggatg taggtctggc aagtaccacg
    14701 agcatggatc atgctcactt cagacgaagg tttacccgca atttgtgcaa cctgcgcccg
    14761 gctcatgcct tttttgacgt ctttcaccac aggctgtaca aactggtctt tggtacgatc
    14821 ataagccgta caacccgcca gcatggttaa taccgccgct gcactcagaa ttcctgccat
    14881 attcttgttc atattccgtc ctcttgttta tcagcgtgtt agataagcct ggaatacatt
    14941 gggcgctttt tcaagcccgt gaacgaaacg gctccgcttt cagaggattc ctgtatgacg
    15001 ttttaaccac cattcagccc gctgtcgctt gtcgtttcag tagcaacggg ttagctttaa
    15061 ggaagttttg tcttttctgt ctggaggggt tcaatgacat tgcaacaaca aataataaag
    15121 gcgctgggcg caaaaccgca gattaatgct gaagaggaaa ttcgtcgtag tatcgatttt
    15181 ctgaaaagct acctgcaaac ttatccgttc attaaatcac tggtgctcgg gatcagcggc
    15241 ggtcaggact ccacgcttgc cggtaagctg tgccagatgg cgattaatga gctgcgccag
    15301 gagaccggca acgaatcact gcaatttatt gccgtacgcc tgccctatgg tgttcaggcc
    15361 gacgaacaag attgccagga tgccattgcc tttattcaac cggatcgcgt attaaccgtt
    15421 aatatcaagg gcgcggtatt ggccagcgaa caggcgctgc gcgaagcggg tattgaatta
    15481 agtgattttg tccgtggcaa tgaaaaagcg cgtgagcgga tgaaagcaca atatagcatt
    15541 gcgggtatga ccagcggtgt cgtggtgggc accgatcatg ctgcagaagc cattaccgga
    15601 ttcttcacta aatatggtga cggcggtacg gatattaatc cgctgtatcg tctcaacaaa
    15661 cgccagggta aacagttact ggcggcatta ggttgcccgg aacaccttta taagaaagcc
    15721 ccaacggccg atctggaaga tgatcgccct tctctgccag atgaagtggc actcggcgtg
    15781 acctatgaca atatcgacga ctatctggaa gggaaaaacc tacctgaaca ggtcgccaga
    15841 acaatagaga actggtatct gaaaaccgaa cataaacgcc gtccgccaat taccgttttc
    15901 gatgatttct ggaaaaagta ataatttgca catattggtt tgtgcgaaaa agagtacttt
    15961 gttcacgccg gatgcggcgt gaacgcctta ctcgacctac aaaactatgc aaattcaata
    16021 cattgcagga gtcgaatagg cctgacaggc gtagcacgtc agacggtgta acctttgtca
    16081 tcgacccgcc tcttttttaa tcgctctccg cctgttacac tggatagata accagcattc
    16141 ggagtcaaca gtggtacggc gtttaacttc tccgcggctc gaatttgaag ctgcggcaat
    16201 ttatgaatat cccgaacatt tacgttcatt ccttaatgac ttacccaccc gaccaggggt
    16261 gtatctgttt catggcgaaa gtgacaccat gccgctctat atcggcaaaa gcgttaatat
    16321 ccgcagtcgg gtgctttctc atttacgcac cccggatgaa gccgccatgc tacggcaatc
    16381 ccggcgaatt agctggatct gcaccgcagg cgaaatcggc gctctgctcc ttgaagcgcg
    16441 attaatcaaa gaacaacagc cgctgtttaa taaacggttg cgccgcaatc gccagctctg
    16501 tgccctgcaa ttaaatgaaa agcgcgtcga tgtggtgtat gccaaagagg tggatttttc
    16561 acgagccccc aacttgtttg gcctgtttgc caataggcgc gcagctttgc aagcattgca
    16621 gagcatcgct gatgaacaaa aactttgtta tggcctgctg ggactggaac cgttaagccg
    16681 cgggcgtgct tgttttcgtt ctgcattaaa acgttgcgct ggggcgtgtt gcggtaaaga
    16741 gagccatgag gaacatgcgc tacgtttgcg ccagtctctg gaacgtttgc gagtagtttg
    16801 ttggccgtgg aatggtgcgg tggcgctgaa agaacagcac ccggaaatga ctcaatatca
    16861 tattattcaa aactggctgt ggctgggggc ggttaattcg ctggaagaag cgacaacgtt
    16921 aattcgggca cccgccgggt ttgatcacga cggttataaa attctttgta agccgctgct
    16981 ttccggtaac tatgaaatta ctgaacttga tccggtgaat aaccagcaag ccagttgatt
    17041 tcactgaaca acagtttacc ttgcggttgt aaaagccgca gggtgtgcct gccatcgaac
    17101 cagcacgcgc cctggtcggt agtgagcagt ttatcgccca gttgccacgc gccgttaatc
    17161 acaaacacca ccccgccgcg tgagccaaag gtagtaaacg tacgttcggc aattctgact
    17221 ttcgccttac agacatccag gcgtgtcatg atgttgaaat ccatcgacat ctgccctgcc
    17281 gtcagtttcg ctttaaccac ctggtccgcc gcaaaggcaa aaggctgtaa cggctttagg
    17341 gtatggttaa agcggtctgc gctttcaagg aacatctcgc cgccttccag caacgtcact
    17401 atcctttcca taccaggaaa caaagaaaac tccccattcg ccgcgatgga tgcaatgctg
    17461 gcacgccagt aaaaatcacg ttttgccggt gggaaagtgc aaatttcgcg cgtttcaccg
    17521 gcagcgtttc gccacaaatt caccgacaat ttacgcatat caaagtattc catactcgct
    17581 cccggcccgt tcttttcatt gttatcgttc aggtaaccgt ggatggttat cggcaactcg
    17641 ggtgatttac gttagtggcg atcaggcttt ttttgcatct gtttgagaac aaaaaagaaa
    17701 accgccgatc ctgtccaccg cattactgca aggtagtgga caagaccggc ggtcttacgt
    17761 tttttggctg aaagattatt cagcagttgc aggcatttta ccttttgccg ctggacgttc
    17821 tgtcagacgc ttctcaaaat tagcattaaa ttgctttttc tgttccggcg tcaggatgtt
    17881 gtaaatcttg ttctgggttt ccatgtgcgc cagcatgtta gctttgcgct gttcttccat
    17941 ttttgcgatc tgcgcttcag cttttgcttt atcgaaggta tcgctggcaa tgatgtcatg
    18001 cattgcgcgg cgttcttcca gcggcggacg tttcatctgg tcacgctggc ctttcatgat
    18061 ttcgcggatc tgctgtttct gcgcgtcggt caggttcagg tctttgaaca tcatgtcctg
    18121 atgcggaccg aacttgcctt tgtggtgcat catcggcttc gcgtcagccg gtgctgaggt
    18181 agtggtgtct gcggcatggg ccaggttagc cgcgccaaga gccaaggtag aggcaacaaa
    18241 cagtgcagtt aatttacgca tattctatat ccttcctttc agttatttat tacggctttc
    18301 ttaagtagcg tgccgtgttg acgagattaa ctttactgac tttagcgtca attaatcaga
    18361 gcaacggtaa aacaatgaaa gtgtaaaaaa cacttttgcg ccaattatgg agaaaaaaag
    18421 aaaatttgat ggagagtaat gagagaatat tacaacacga tgattttgca gagattatga
    18481 agaactatac cggatgactg gtgataaata aagcaaataa ccaggattaa tctgtattaa
    18541 tttataagaa agtaacttaa tacccgcaga atgatttctg cgggtaagta ttagcttatt
    18601 ttttcgagca ttaatcccgc gcgtaatccc aacgctacca aaggattagg gaataacaca
    18661 tactctacat catgggttac ggtaaaacgt tcctccccgt cctgcgccag caatgttcct
    18721 ttctcaaacg gcataaaatt cagcgtgtca cttgccatat gcatttcgaa ggacggcgag
    18781 tggcgagtaa tttgcgaaac cacccgataa cggagcggcg gcgttctcac gataccgaca
    18841 ctctcaccag atagcagcgc agcaattgcg ctggcagtga ctgcaaactg gcgaagatcg
    18901 ttttgtccaa agggcaacgc tttgccgagt tccagcgtac aggccagcgc gccaaaatgt
    18961 ctggcgctga aatgggtaaa cgtaccacca ggctcctgat ggaataccag cgcctccagc
    19021 cccgccgcac ccagccacgt cagaaatttc tcgtcccagg gaatgtcgcg ttgcggtaat
    19081 acaccgaact gcggatgcaa ggagccacga attgcggtat gtagatcaag gtgccagcgc
    19141 acagattctt tgccctggtc ataaaaatct tccaggcact gctccagttc gcgcgcccga
    19201 caggtttccc cgctttcagc aaatagctgc caacgaccgc caaacattcg gttcatatcg
    19261 ctatggcaat aacgtttccc ttgcttcagc gcaggaggat tcccgaggat caccagcaac
    19321 cgccaacgta acgggatctc gccgtgagat atcgcgccaa gcaacgcgtc cagcatttcc
    19381 acaggagccg tctcattacc gtggatcccc gctgaaatca ccagtgcgcc ttgcggtggc
    19441 gttaatggcg tcagttccag cacgccatcg cccagccagc gccagcgaac gccgttgatt
    19501 tctcgctcgg tgataaccgg ttttttaccc gttaaggtca gagcaagaaa attatccatt
    19561 tccccctccc tcgcgctgga acggataaac cgaaccgaga ttcagtaatt ggctcaatac
    19621 atccagtgct tcccgccctt cgcgcagcaa ttgcgggtcg gccagatcgg cagcagtaag
    19681 gcgatcgcgg tagtaacgat ccacccagtc attgagcgca ttaaacagcg tatcgttcat
    19741 catcaccgcc ggattcaccg cccggcgttc ttcttctgtc aataccacgc gcaaccgcag
    19801 gcacgccggg ccgccgccat tcgccatgct ttcacgtaaa tcaaagactt ttagttcgct
    19861 aatcgggttg tcagcggcaa ggagttcatt gagataaccc catactccgg cgtgtttccg
    19921 acactcctga ggcagcacca acatcatgga accatcatcg cggctcagca gttggctgtt
    19981 aaacagatat gtagacaccg catctgacac ggaaacctga gtcgccggaa cttctatcgc
    20041 cataaaaccg ttcacccgcg cacgcaggct tgccagtaac tgtgactggc gagcgaacgc
    20101 ctgttgatgg caaaacagca cctggcggtt actcacggca atcacgtcat tatgaaaaac
    20161 gccctggtcg ataacgtccg ggttttgctg ggcgaaaatc acctgttggg gattcacctg
    20221 attcagcctt gccaccgcct cgctggcttc gcgagtctgc cgcgccggat aacgggaagg
    20281 ccgggtatca ttgccttctt ctcgcccgta gacaaaaagt tgcatacccg gttcaccgta
    20341 atgaccgccg agacgattgt ggtttgccgc cccctcatca ccgagcaacg ctacctgcgg
    20401 caacgccgaa tggacactaa atttctcttc gtcgttaaaa atcgctttta acaacgattc
    20461 ggtgacgggg gcttccagcg aacggtgaaa tttattgttc aggttggcaa cggtgagatg
    20521 cactttgcca tccagcgtat cggcagatgg cgcgatcgtt gccgcattgg ctacccacat
    20581 tggcgaagcg gagctgacgc tggaaagcca gtgcggtgcc tggcgggcaa ccttttccag
    20641 tacctgctca tcgctgccgc tgaatcccaa ctgacgcagc accggaataa acggacgctc
    20701 gtgcggcggg atcaccgcct gggggaatcc cgcgtcggca agggctttca ttttcagtaa
    20761 gccctgcttc gccgccagtc gcgggttaga gacctgaaaa cggtgacggg tggaggcttc
    20821 attaccaaac gacaggcccg cgtaatgatg cgtcagccct accagcccgt cgaaattgat
    20881 ttcccaggcg ttcatcgcac cacctcatcg gaaaaatcca gcccggggtt aagcgttgcg
    20941 ggcaatgtta acgagtccga ctccagactt gccatcggcc aggcacaata atctgcggca
    21001 taccaggcgc tggggcgatg gttaccggaa gcgccaatgc cgccgaatgg cgcggtactg
    21061 gcagcaccgg taagcggttt gttccagtta acaatccccg cccgtgcctc cagcaacagt
    21121 tgatcgaatt tttcccgctc gggggaaacc agaccgcaag agaggccgaa gcgagtgtta
    21181 ttcgccattc gaatcgcttc atcgaaattg tcataacgcc agacgcgcaa taacggccca
    21241 aacacttctt catccggcac gccagtaacg cctgtcattt caatgatccc cggcgtcagc
    21301 aacgatgtcc ctgcttgtaa taagcgcggc gcaagcaggg tccgtccgcc catcgcctcc
    21361 agttcctgcc aggcagtaac cacctgctgt gcggcctgct cagaaatcag cccgccaata
    21421 aacggctgcg gttcgtcatc ccaacggccc ggcgttaacc gctggctgac ggcaaccagg
    21481 cgagcaagaa atgcatcgcc ctgcgttcca cttttcagca ataaacggcg ggcgcaggtg
    21541 cagcgttgcc cggcagtgac aaacgccgac tgaatggtca gatggacagc ggcatcgata
    21601 tccgccacct catcgataat tagcgggtta ttaccgccca tctcaagggc gagaattttc
    21661 tccggctgac cggaaagctg gcgatgcagc tggtagccgg tattggcgct gccggtaaac
    21721 agcaagccgt cgagatcctc cagcgcactc agcgcctgac ccgtttcacg cccgccctgc
    21781 accaggttca gcacgcccgg cggcaagcca gcctgctgcc ataaacgcat taccgcttcg
    21841 ccactccacg gtgtcagttc gctgggttta aagataatgg tgttacctgc cagcaatgcc
    21901 ggaacgatat gtccgttcgg taaatgaccg gggaaattat acggcccaaa caccgccagc
    21961 acgccgtgcg ggcgatgtcg caggctcgcc gcgccgtccg gcatttcact acgctgctcg
    22021 ccggtacgaa cgtgatacgc cttaattgat atcgcgattt tattgatcat cgccatcact
    22081 tcggttgccg cttcccagcg cggcttaccc gtttctctgg caataatcgc ggttaattcg
    22141 gctttattgc tttccagcaa tccggcaaag cgttcaactc ttacctgacg atcgccaaag
    22201 gagagacgcg cccagcgcgg aaacgccgca cgggcagccc gacaagcctg cccgacctga
    22261 gcggcatcgg catcattgcc ttgccataac acctcgcccg ataccggatt acgcttcaca
    22321 cgcgatgcgc cctggcccgt aacccagtca ccattgatcc ataaagtcat gctgttttct
    22381 cctctgcgca caggcgcacc agacgaacgc gatccccggc gtggcatttg agagcatcca
    22441 gttgtgcagc ggttaatatc aaacgctcgg tcgctggatc ggtacgcacc agcaccacgc
    22501 ggaaatggtg ataattttca ttggcgacca ggcaggccgg gaattcgccc tgcgcaggct
    22561 gcccttctgc cacttccacc agccgacttt tacggatggc gcgcacgcgg tcgatatcac
    22621 attcaagtgt cggcccgccg tcaaagatgt cgatatagtt acggtagcga aaaccttctt
    22681 tctccagcac cgcacgggca ggcgcggttt gcggatgtac ctgaccgatg acgtcctggg
    22741 cttcctggga taaaaagtgg gtatagatcg gatgtttcgg catcagttct gcaataaacg
    22801 ccttttgccc ggtgccgcag agaaaatcgg cgcggctaaa atccatcgaa aagaagcgtt
    22861 taccgaggct ttgccagaac ggtgaatagc cgtgttcgtc aatcaccccg cgcatttcag
    22921 caaccacttt gtcattaaac ttgtcgcgaa aagccgccat aaacataaag cgcgatttcg
    22981 acagcaaata gccgttgccc tctttgcgcc actccgggtc gagaaacagc gtgcacagct
    23041 cgctgctgcc ggtgtgatcg ttactgagaa acagcgtcgg caatgcgtta tagacattca
    23101 gctcttttga ggcgtgaacc aacgtgccga cgcgatagtt gtaccaggga tcgttcagcc
    23161 caactgccac ctcaatggca caaatccccg ccactgtgcc tgtctcgcta tcttccagca
    23221 cgaacacata gccctgctca cttttgggca gttcgccttg ccaggttttg attgcccttt
    23281 cgatacgcgc cgaaagcgtg gcttcattgg cgggaagcga cgtcaggccg ccgcccgttt
    23341 tgctggcaag ctgcattaac gctgagacat cagtgcgttc aacgggacgg atgaccatca
    23401 tgatgaacct ccgctcacaa agcgttcaca ggccacagca aaacgatcca gcccggtcgt
    23461 cacctcttct tcgctgacat tgagcgcagg cgcaaaacgc accacattgc cacccgcaat
    23521 cagtaccatc acgcccgctt tcgccgcctc ctgagaaatt tgtttcgctt gcccggcgta
    23581 atccgcattc agcacacagc caatcagcag acctaatccg cgaatttcac tgaacaaacc
    23641 gcagcggtga ttaatggtat taagacgctc aacaaaccag tcgtgacgct gtttaacgcc
    23701 attaagcatc tctggcgtgt tgatgagatc cagcacttta cctgccaccg ccgaggccag
    23761 cggattaccg ccataagtgg taccatgtgt gcccacggtc atcacgctgg cgcattcttc
    23821 ggttgccaac agagcgccaa cggggaaacc gccgcccagt gctttggcgg tagttaacag
    23881 atcgggcgtc acgccgtagt gcatataggc atacagttcc ccggtgcgcc cgacgccggt
    23941 ttgtacttca tcaaaaatca acagcgcatt gtggcggtca cacaattcac gcagaccttg
    24001 taaaaatgcg ttgcttgctg gcaccacacc gccttccccc tggatgggtt cgacaatcac
    24061 cgcacaggta gagtcgtcaa tcagcgcgct ggcagagtta atatcgttat atgcggcatg
    24121 acgaatatcc ggcggcagtg gcgcaaaatc ctgtgaatag gctggctgac cgcccgcact
    24181 gacggtaaac agcgtgcgac catgaaacgc atttttaaac gccacgatac cgctcttatg
    24241 gctgccgtag cggtcgtgag cgaatttacg cgccagtttt agcgccgctt cgttggcttc
    24301 cgcaccggag ttacaaaaga agacgcgata ggcaaacgtg gcgtcgatca attttttcgc
    24361 cagtcgcagt accggctcgt tggtgtaacc gttgccggta tgccagaact tactcgcctg
    24421 ttcgttcagc gcctcacgca gttccggatg cgcatggccc agcgcgttca ccgcaatgcc
    24481 acccgcgaag tcgatatact ctttcccctg ctgatcccac aagcgcgaac cttcgccacg
    24541 taccggtata aacggtgccg gagcgtaaac aggtatcatc cattcatcaa agttttcacg
    24601 cgtaattggc tgagacatag cgacctctac agtaaataa
//
LOCUS       DAPHCV010000077        24303 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.077, whole genome shotgun sequence.
ACCESSION   DAPHCV010000077 DAPHCV010000000
VERSION     DAPHCV010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.077"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            95..439
                     /locus_tag="RH033_003076"
     CDS             95..439
                     /locus_tag="RH033_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin biosynthesis transcription regulatory
                     family protein"
                     /protein_id="HDV2992228.1"
                     /translation="MNSEVCLHLVKERSGILLPGEVSERHFWLLIEISGMHSEKVINA
                     LREHLVMGYTRREACEQHGVSQGYLSGALVRIQHLSQTVNNLLPYYVSGLAMNHEEEG
                     DRSVKRGGDWRV"
     gene            517..1284
                     /locus_tag="RH033_003077"
     CDS             517..1284
                     /locus_tag="RH033_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDV2992229.1"
                     /translation="MKIRHLAALVGILAGLPASGVMAAGNQESTKTTSKVFSLHLGAT
                     RVIYNLDSSGASLTVMNDLDYPMLVQSEVLAENQKDPAPFVVTPPLFRLDALQSSRLR
                     IVRTGGVFPEDRESLQWLCVKGIPPKHDDRWAEEKGADKKKADKATLQVNLSVSSCVK
                     LFVRPPAVKGRPEDAGGKVEWTKSGNKLKGNNPTPFYINISELSVGGKEVKEHRYISP
                     YSSYEYEIPSGASGKVSWTVITDYGGKSKPFESELKS"
     gene            1531..4041
                     /locus_tag="RH033_003078"
     CDS             1531..4041
                     /locus_tag="RH033_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004719950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HDV2992230.1"
                     /translation="MAACTLLYTGNTEARTYSFDASMLKGGGKGVDLTLFEEGAQLPG
                     IYPIDIILNGSRVDSRDMAFRTEKDAEGKTYLKTCLTREMLAHYGVMTEEYPELFHGD
                     DGKEADACAELSVIPQATETYQFASQQLLLSIPQVALRPPLRGIAPEALWDDGIPAFL
                     LNWQANTSRSEYRGYGKSVTDNYWVTLEPGINLGPWRVRNLTTWNRSSGQPGLWESAY
                     IRAERGINSLRSRLTLGEDYTPSDIFDSVPFRGVMLGSDESMVPYNQREFAPVVRGIA
                     RTQARIEVRQNGYLIDSRTVAPGAFALTDLPLTGSGGDLQVTVQESDGTAQVFTVPYT
                     TPAIALREGYMKYSIAGGEYRSSDDAVEHSPLGQMSVMYGLPWGLTAFGGAQMSSHYQ
                     SAALGLGWSMGRLGAVSVDGIHSRGQQKGRDTETGETWRLRYNKSFELTNTDLTAARY
                     QYTSSGFHTLSDVLSTFRNDNFRAYSYSGDRSRRTTLRLNQYLGSLGYMSFYGSRDEF
                     RHNRQKQDSFGMSYGTSWKNISWYVNWSRNYSTNAYYQRGHIEDSINLWMNIPLGQWI
                     GGRDNDINVTTQTQRTTGQNTWYETGLNGRAFDRRLYWDVSERIAPGSENNVDSSRLN
                     LRWFGTYGELAGMYSYSSYIRQMSAGISGGMVLHNEGITLGQKGEDTVALVVAPGVNG
                     ASVGSLPGVRTDTRGYTLVNHVSPYQENLITLDPTTFPENVEVSQTDTRVVPTKGAVV
                     RAKFITRVGGRALITLSRSDGSRLPFGAVVTEEGKKGQMPGGAGVVGDNGEVYLSGLD
                     ETGQLKVQWGRNSSCRANYRLPEEKEATGIFLVRTVCM"
     gene            4057..4476
                     /locus_tag="RH033_003079"
     CDS             4057..4476
                     /locus_tag="RH033_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDV2992231.1"
                     /translation="MKKIQIVCSGIVLVVISSLAQAVELSLNTSDGRSGELKDGTKVA
                     TGRIICRGTYTNFHIWMNSRQMGNIPGHYIILGRHDSHNEMRVRLDGAGWLPSVSDGQ
                     GMVSTGIPEQHTFDVVIDGNQLLEPDEYILSVSGECS"
     gene            5011..5523
                     /gene="bmaE"
                     /locus_tag="RH033_003080"
                     /gene_synonym="afaE-8"
     CDS             5011..5523
                     /gene="bmaE"
                     /locus_tag="RH033_003080"
                     /gene_synonym="afaE-8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M agglutinin-type afimbrial adhesin BmaE/AfaE-8"
                     /protein_id="HDV2992232.1"
                     /translation="MNLKKIAIASSVIAGITMALTCHAVTVTATHTVESDAEFTIDWV
                     DAGPTTTDAKDGEVWGHLDMTQTKGTPTFGKLRNPQGETSPGPLKAPFSFTGPDGHTA
                     RAYLSSYGAPIHNYAGDNLANGVKVGSGSGNVPFVVGETSRLTAKIFGDQTLVPGVYR
                     TTFELTTWTD"
     gene            complement(5818..5997)
                     /locus_tag="RH033_003081"
     CDS             complement(5818..5997)
                     /locus_tag="RH033_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDV2992233.1"
                     /translation="MYAYSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRERF
                     ISPFEYAKINNPLKQ"
     gene            6078..6458
                     /locus_tag="RH033_003082"
     CDS             6078..6458
                     /locus_tag="RH033_003082"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDV2992234.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            6455..6802
                     /gene="tnpB"
                     /locus_tag="RH033_003083"
     CDS             6455..6802
                     /gene="tnpB"
                     /locus_tag="RH033_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDV2992235.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            6852..8390
                     /locus_tag="RH033_003084"
     CDS             6852..8390
                     /locus_tag="RH033_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDV2992236.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRNGIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLSRHSELAKAFAYALNQWPALTYYADDGWAEADNNIAENALRMVSLGRKN
                     YLFFGSDHGGERGALLYSLIGTCKLNGVEPESYLRYVLDVIADWPINRVGELLPWRVA
                     LPTE"
     gene            8648..8953
                     /locus_tag="RH033_003085"
     CDS             8648..8953
                     /locus_tag="RH033_003085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992237.1"
                     /translation="MSYQPGGLVLPDLSRRGYLSKNASSSNRFGLVNGDGLSKDSRCW
                     CTFSRWRSSMDNAIACSSSLVIVCTWKAIYRSTSHYGKNVFIVATCVIIQVSLERPV"
     gene            complement(9551..10779)
                     /locus_tag="RH033_003086"
     CDS             complement(join(9551..10464,10464..10779))
                     /locus_tag="RH033_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDV2992238.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRGKKVDSARALIARGWGVSFVSRCLRVSRAQLHVILRRTDDWKDGRRSRHTDDTDV
                     LRRIHHVIGELPTYGYRRVWALLRRQTELDGMPAINAKRVYRIMRQNALLLERKPAVP
                     PSKRAHTGRVAVKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTGGFD
                     SETVQDVMLGAVERRFGSELPASPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRS
                     PESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREY
                     LRQRASNGLSDNRCLEI"
     gene            complement(10975..12021)
                     /gene="fbpC"
                     /locus_tag="RH033_003087"
     CDS             complement(10975..12021)
                     /gene="fbpC"
                     /locus_tag="RH033_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HDV2992239.1"
                     /translation="MSQKNFVELRNVTKRFGSNMVIDNINLTIPQGQMVTLLGPSGCG
                     KTTILRLVAGLEKPSEGQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYG
                     LKMLGVSRNEVKQRVKDALAMVDLEGFEDRYVDQISGGQQQRVALARALILKPKVLLF
                     DEPLSNLDANLRRSMRDKIRELQKQFNITSLYVTHDQSEAFAVSDTVLVMNKGHIMQI
                     GSPQELYRQPASRFMASFMGDANLFPANFSEEYVDIYGYRLPRAAYFPVQGRGTVGVR
                     PEAITLSNHGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDTGEHYYL
                     EIHPYGMFVLADSA"
     gene            complement(12033..14111)
                     /locus_tag="RH033_003088"
     CDS             complement(12033..14111)
                     /locus_tag="RH033_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDV2992240.1"
                     /translation="MSQTLALHPVKKRDAIFLWVLLGWLAFILLPSWSLDYGLLESTS
                     DEILEAYGWSHFNISWLWYLLPSLLLVRPFNEAKREQRSRHYFDAGWALLCIAFMVIS
                     ATIVGRGLGYAAIVLFIELSAIMTLALTRLEWLGGDRFVIGSLIAIVALIGIFIVWPS
                     IAIFIPMFANDAGEFAPLAFMNVLSQAHIIQVIVNSIVLSIAVGIGCTFFGLVLAIYT
                     TRIAKRSSVIGRIFSILPIVTPPFVVGLGVTLMMGRSGYITEFMVEWFGLTNTNWLYG
                     FTGIWLAQVLAFTPMAFMILDGAIKTIHPSLEEASYTLRASRWQTFSGVFVPLLKPAL
                     ANAFLIVVVQSLADFSNPLVLGGNFDVLATQIYFYITGSQLDYQAASTLGAFLLLFSL
                     LVFCVQYMWIGKRSYVTVSGKSYRGDVQPLPVTLVCSVVAILAIWIAFNALLYGSIFY
                     GSFTVNWGVDYTLTLDNFIKLFGQGMSDGAWPSLLDTLLYAGIAAPITAIFGLLIAWV
                     VVRQQFKGKKTIEFTTMLCFAVPGTVAGVSYILAFNSAPVYLTGTAAIVIISMVMRNV
                     PVGIRAGIAGLGQIDKSLDEASLSLRAGSLRTITHILLPLLRPAILSALIYSFVRAIT
                     TVSAIVFLVTPDTRVATAYILNRVEDGEYGVAIAYGSILIVVMLAIIFLFDWLIGEAR
                     ISRSKAKNQA"
     gene            complement(14180..15211)
                     /locus_tag="RH033_003089"
     CDS             complement(14180..15211)
                     /locus_tag="RH033_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV2992241.1"
                     /translation="MKMTFTSTLIASAVALATLTGAAQAKGRLVVYCSATNEMCEAET
                     KAFGEKYDVKTSFIRNGSGSTLAKVDAEKKNPQADVWYGGTLDPQSQAGEMGLLQAYK
                     SPNLEQIMEKFRDPAKVKGNLSSAVYVGILGFGVNTDRLKEKNLPVPKCWKDLTKPEY
                     KGEIQIADPQSSGTAYTALATFAQLWGEDQAFDYLKQLNANVSQYTKSGIAPARNAAR
                     GETAIGIGFLHDYSLEKEQGAPLELISPCEGTGYEIGGVSILKGARNLDNAKLFVDWV
                     LSKEAQELAWKKGKSYQILTNTTAETSPNSLKLDDMKLINYDMDKYGSTDVRKALINK
                     WVSEVKMGK"
     gene            complement(15208..16524)
                     /gene="uhpC"
                     /locus_tag="RH033_003090"
     CDS             complement(15208..16524)
                     /gene="uhpC"
                     /locus_tag="RH033_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HDV2992242.1"
                     /translation="METTMHARSAREINQCYRTLRPRLLMCMIIGYAAFYLTRKSVNY
                     VLPALQTDLGLDKGDIGLLGSLFYLSYGLSKFTAGLWHDSHGQRGFMGVGLFATGLLN
                     VVFAFGESLTLLLVVWTLNGFFQGWGWPPCARLLTHWYSRNERGFWWGCWNMSINIGG
                     AIIPLISAFAAHWWGWQAAMLTPGIISMELGIWLTLQLKGTPQEEGLPTVGHWRHDPL
                     ELRQEQQSPPMGLWQMLRTTMLQNPLIWLLGVSYVLVYVIRIALNDWGNIWLTESHGV
                     NLLSANATVMLFEVGGLLGALFAGWGSDLLFSGQRAPMILLFTLGLMVSVAALWLAPV
                     HHYALLAVCFFTVGFFVFGPQMLIGLAAVECGHKAAAGSITGFLGLFAYLGAALAGWP
                     LSLVIERYGWPGMFSLLSVAAVLMGLLLMPLLMAGITTTHARRIKQ"
     gene            complement(16597..18138)
                     /locus_tag="RH033_003091"
     CDS             complement(16597..18138)
                     /locus_tag="RH033_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HDV2992243.1"
                     /translation="MSRNLRHWVISLFIVLAWGTGWLMLWTLSFYLTNNGQQAVLFLP
                     QGVYLALVILLSRRYWPALVLPPLLMMFWLHSEQLLNGYLMLATPVISLFPVLLAQNF
                     WHRFPLYWQRLTLLLATVTAASLLNTALLSPFMSGPIMLPGLTSFTGGVLLTPFVYLI
                     FEFLRQQHRYQLLGLDTHNPPLRTSLIIWCSLFFIIGIGTQMVLSPEIERLLLIVVFL
                     PNVVMAWKFGWQGGVLSGLLGSMMITIARQIGVGFSNLVELEIFLATQALLGIGLGIA
                     ISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKNLARELHDEIGQNITAI
                     QIQSQLVKRARDPAQIQSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHL
                     INEFAFSERGIHCQFAYQLNSTPENETVRFTLYRLLQELLNNICKHAEASEVTIILRQ
                     QGEVLHLEVSDNGVGIASGKMAGFGIQGMRERVSALGGDLTLEKQHGTRVIVNLPTFL
                     QQIAG"
     gene            complement(18138..18767)
                     /locus_tag="RH033_003092"
     CDS             complement(18138..18767)
                     /locus_tag="RH033_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDV2992244.1"
                     /translation="MIRVVLVDDHVVVRSGFAQLLSLEDDLEVIGQYSSAAQAWSALI
                     CDDVNVAVIDIAMPDENGLSLVKRLRAQKPQFRAIILSIYDTPTFVQSALDAGASGYL
                     TKRCGPEELVQAVRSVGMGGHYLCADAIRALRGGGQPAQALEILTPREREVFDLLVKG
                     DSVKEIAFKLELSHKTVHVHRANVLGKLNCHSTIELVHFALDHHLLAGH"
     gene            complement(19050..20231)
                     /locus_tag="RH033_003093"
     CDS             complement(19050..20231)
                     /locus_tag="RH033_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HDV2992245.1"
                     /translation="MPLTVRQIESAKPADKDYKLSDGQGLYLLIKKSGARYWNLKYRF
                     AGKEKKLVIGVYPGISLALARQKRDEARKLLAEGKDPSYEKQASKHALRESIACTFCT
                     LAEELFTHMACRWTAGYLADQRARFENHINPMLGKRPVADIRPLEILNALRVIEKNGK
                     LEALRKTRQLCSQIFDYAIATGRATINPASTLNSVVLHPEKQQRRMLSEEQLGAFMYQ
                     MKVSGNFMSLVTQILVLTAVRTGELRLATWDEIDFTNALWVIPKERMKMRRPHVVPLS
                     HQTQMLLKEIKIITAPRNSELVIPGVYRDNQPRGRGCINEFLQQVGWHTFTTAHGFRH
                     TFSTILHDHDFSSAWIELQLAHVDKNIMRGTYNHAQYLDGRREMMQWYADYIDSLSEQ
                     A"
     gene            20406..21221
                     /gene="ulaE"
                     /locus_tag="RH033_003094"
     CDS             20406..21221
                     /gene="ulaE"
                     /locus_tag="RH033_003094"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001692974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HDV2992246.1"
                     /translation="MLPAGECWLERLQLAKTLGFDFVEISVDETDDRLSRLDWSREQR
                     LALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKAIQFAQDVGIRVIQ
                     LAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYPLMNSISKALGYAH
                     YLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPGVFKNVPFGEGVVD
                     FERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMAKAGMVEAA"
     gene            21221..21907
                     /gene="ulaF"
                     /locus_tag="RH033_003095"
     CDS             21221..21907
                     /gene="ulaF"
                     /locus_tag="RH033_003095"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HDV2992247.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARGINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(22036..22311)
                     /locus_tag="RH033_003096"
     CDS             complement(22036..22311)
                     /locus_tag="RH033_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV2992248.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            22639..23034
                     /gene="rpsF"
                     /locus_tag="RH033_003097"
     CDS             22639..23034
                     /gene="rpsF"
                     /locus_tag="RH033_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDV2992249.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            23041..23355
                     /gene="priB"
                     /locus_tag="RH033_003098"
     CDS             23041..23355
                     /gene="priB"
                     /locus_tag="RH033_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HDV2992250.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            23360..23587
                     /gene="rpsR"
                     /locus_tag="RH033_003099"
     CDS             23360..23587
                     /gene="rpsR"
                     /locus_tag="RH033_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDV2992251.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            23629..24078
                     /gene="rplI"
                     /locus_tag="RH033_003100"
     CDS             23629..24078
                     /gene="rplI"
                     /locus_tag="RH033_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDV2992252.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
BASE COUNT         6143 a         5880 c         6456 g         5824 t
ORIGIN      
        1 tccatggtaa tattggtggc gaggcagggg attaccggtg ttcattccgg ctctgatgct
       61 gagagtttgt ttccctgtca gagtaaacgg ggttatgaat tcggaggttt gtttgcatct
      121 ggtgaaagag cggtccggga ttctgctgcc cggggaagta tcagaaaggc atttctggtt
      181 attgattgaa atatccggga tgcacagtga gaaagtgatt aatgcgctca gggagcatct
      241 tgttatggga tatacccgca gggaggcctg tgagcagcac ggtgtttctc aggggtattt
      301 atcaggagcg ctggttcgga ttcagcacct cagtcagacg gtgaataatc tgcttcctta
      361 ttatgtttct ggtttggcaa tgaaccatga agaggagggt gatcggtcag taaagcgtgg
      421 tggtgactgg cgggtctgag gggatactgg tgacagctgt cgggcggttg ctattaacat
      481 atttataaaa ggtttatttg ccttcaggat aaaataatga aaatcagaca tcttgcagcg
      541 ctcgttggca tactggcggg gttgccggct tccggcgtaa tggcagccgg aaaccaggaa
      601 agcacgaaga ctacgtcaaa ggttttttcc ctgcatctgg gggccacaag ggtgatttac
      661 aacctggatt catcgggggc ctcactgacg gtaatgaatg acctggatta tccgatgctg
      721 gttcagtcag aggtgctggc agagaatcag aaggacccgg ccccctttgt ggtgacgccg
      781 ccgcttttcc gtctggatgc acttcagtcg agccgtttgc gaattgtccg taccgggggt
      841 gtttttcctg aggaccgtga gagtctgcag tggctttgtg tgaaaggcat tccgccaaag
      901 catgatgacc gatgggcaga agagaaaggt gctgataaaa agaaagctga caaggcaacc
      961 ctgcaggtaa atctttccgt cagcagttgt gtgaagctgt ttgttcgtcc gcctgcggtt
     1021 aaagggcgtc cggaggatgc aggcggaaaa gtggagtgga caaaatcagg taataaactg
     1081 aaagggaata acccgacacc attttatatt aatatttcag agctgagcgt tggtgggaaa
     1141 gaggttaagg agcatcgtta tatttctccg tactcctctt acgaatatga aattccttct
     1201 ggtgcatcag gaaaagtcag ctggacagtg attacggatt acggcggaaa gagtaagccg
     1261 tttgaatcgg aattaaaaag ctgaaagccg gaataaatat taacgtcaga ggcgggctta
     1321 tttgattaac ccggttgaat atctgagcgt gtgtgaaata agtgtgtgtg aacaggcgtg
     1381 caaacgcaca aatacgtgtg tatatcctcc ggcatatgta aataaaaaga aaccgtggct
     1441 caggtgtgaa taatgcacag gaataagtca tcttcaggtt tatctttata ttgcgtaaaa
     1501 acaaaaatta cagcggttgc tctctctgtg atggcggcct gtacccttct ttacaccggg
     1561 aatactgagg cccgcacata ctcctttgat gcttccatgc ttaaaggtgg cggaaagggg
     1621 gtggacctga cgttgtttga agaaggtgcc caactgcccg gtatctatcc gattgatatt
     1681 atcctgaacg gttcgcgggt ggattcgcgg gacatggcgt tccgtacgga gaaagatgcg
     1741 gagggtaaga cttatctgaa aacctgtctg acccgggaaa tgctggccca ctatggtgtc
     1801 atgacggagg aatatccgga gttgtttcac ggggatgacg gaaaggaagc tgacgcctgt
     1861 gctgagttat ccgttattcc acaggccacg gaaacatacc agtttgcgtc acagcagttg
     1921 ctgctgagta ttcctcaggt ggccctgcgc ccgccactga gggggatagc gccggaggcc
     1981 ttatgggatg atggtattcc tgcttttctg ctgaactggc aggcaaatac cagtcgttca
     2041 gaatatcgtg gatacgggaa atcggttact gacaattact gggtcactct tgagccgggc
     2101 attaatcttg gtccctggcg ggtgcgtaac ctgacaacct ggaacaggtc atccggtcag
     2161 ccggggctgt gggagagtgc ctatattcgt gcggagcggg ggattaacag tctcagaagt
     2221 cgtctgacgc tgggtgagga ttacacgcct tcagacattt ttgacagtgt gccgttccgt
     2281 ggggtgatgc tggggtcaga tgagagcatg gtgccttata accagcgtga atttgcgccg
     2341 gtggtcagag gtattgcccg gacccaggcc aggattgagg tgcgccagaa tggttatctg
     2401 attgacagcc ggacggtggc cccgggcgca tttgccctga cggacctgcc gctgacgggg
     2461 tcaggtggag accttcaggt aacggtacag gaatctgatg gtactgctca ggtgtttacg
     2521 gtgccttaca ccacaccggc cattgcgctt cgtgagggat atatgaagta cagcattgcc
     2581 gggggggagt accgttcttc agatgatgct gttgagcatt caccgctggg gcagatgagt
     2641 gtcatgtatg gtctgccatg ggggctgacg gccttcgggg gagcacagat gtcatcgcat
     2701 tatcagtcag cggctctggg gctgggatgg tcaatgggca gactgggggc ggtgtcggtt
     2761 gacggcattc attcccgcgg tcagcagaag ggacgggata cggagacggg agagacatgg
     2821 cgtctgcgtt acaacaagtc atttgaactt acgaatacag atttgacagc ggcgagatat
     2881 cagtatacgt cctcaggctt ccacacattg tccgatgtgc tgagtacatt tagaaatgat
     2941 aattttcgtg catatagtta tagtggggac aggagtcgcc ggacaacatt acgccttaat
     3001 cagtatctgg gatctctggg gtatatgagc ttttacggta gccgtgatga gttccgtcat
     3061 aaccgacaga agcaggattc tttcggcatg tcatatggaa cgtcatggaa gaatatttcg
     3121 tggtacgtta attggtcacg taactatagt acaaatgcat attatcagag agggcatata
     3181 gaggatagta tcaatctgtg gatgaatatt cctctggggc agtggattgg gggtagagat
     3241 aacgatatta atgttacaac tcaaacgcaa agaactacgg ggcagaatac ttggtacgaa
     3301 accggtttga atggtcgcgc atttgaccgt aggttgtatt gggatgtgag tgagcggata
     3361 gctcccggaa gtgaaaataa tgtagacagt agccgactga atttgaggtg gtttggtacc
     3421 tatggtgagt tggcggggat gtacagttac agcagttata ttcgccagat gagtgctggt
     3481 atatcaggag gtatggtgct ccacaatgaa ggtatcactc ttggtcagaa aggagaggat
     3541 acggttgctc tggttgtagc acctggagtt aatggcgctt cagttgggag tttgcctggc
     3601 gttaggaccg ataccagagg ttacactctt gtgaatcatg tatctccata ccaagaaaat
     3661 cttattacac tggatccgac aactttcccg gaaaatgtgg aagtatcaca gacagatact
     3721 cgtgttgttc ctacaaaggg agccgtggtt cgggcgaagt ttatcacccg agtaggggga
     3781 agagcactga taactctgtc acgtagtgac ggctctcgcc tgccttttgg agctgtggtg
     3841 acagaggaag gaaagaaagg tcaaatgcct ggtggggcag gggttgtggg ggataatgga
     3901 gaagtgtatt tgagcggact ggatgaaacc ggacagttga aggttcaatg ggggcgtaac
     3961 agttcttgcc gtgcaaatta tcgacttcca gaggagaaag aagcaactgg tatttttctg
     4021 gtgcgcactg tgtgtatgtg accaaggagt accctgatga agaaaataca gatagtatgt
     4081 agtggtattg tactggtggt tatatccagt ctggcgcagg cagttgaact gagtcttaat
     4141 accagtgatg gaaggagtgg cgagttaaaa gacggtacga aggtggcaac aggaaggatt
     4201 atctgccgag gcacctatac aaattttcat atctggatga atagcagaca aatgggaaat
     4261 attcctggtc actatattat actgggtaga catgacagtc ataatgaaat gcgggttagg
     4321 ctggatggcg caggatggtt gccatcggta agtgatgggc aaggtatggt cagtaccggg
     4381 atacctgagc agcatacatt tgatgttgtg attgacggaa atcagctgct tgagcctgat
     4441 gaatatatat tatcagttag cggagaatgc tcataatatt cataatttct ttatgctaat
     4501 aaagtaacta ttcagttgtc aatgactgcc ctctaaaata gcatgttatt ttataaaagc
     4561 atgtataact ttattttgct tgttgtaatt taccatattc tatggtccac aacagactta
     4621 aataaaggtg ttatagttga tgtgttaaaa tgcatatgta atgtaagtgt aaactgagtg
     4681 ccgtatgtag ccattaaact tgtattgatg atgcagtgtc caccttttca tcaaaaagtg
     4741 gacgtctgtt atggaacata tgtagctaag tatctgtgaa aatatattga acgttaagtt
     4801 taatgaacgg gcagctcctg ttgtagttga ctgtacatta aaatatcact ggtggaagca
     4861 tttaaatatt tatatgtatc ctgttggggg acttaatgta ttcgtgtttt cctgcagggt
     4921 atttaaatag cgggccagtg tttattgtgg tgaagcagta ttatagaaat atgttttgcc
     4981 atttattata accaagtata aggataaggt atgaatttaa agaaaatagc tatcgcaagc
     5041 agtgtcattg caggtataac aatggcgcta acttgccatg ctgtgacagt aacagccact
     5101 catacagttg aatcagatgc tgaattcaca atagattggg tcgacgctgg gccaacgact
     5161 acagatgcaa aagatggtga ggtttggggg caccttgata tgactcaaac caagggaaca
     5221 ccaacattcg gaaaactccg caatcctcaa ggagagactt cgccaggacc gttgaaggcg
     5281 ccattcagtt ttaccgggcc agatggtcat actgcaagag cgtaccttag ttcatacggc
     5341 gcaccgattc acaactacgc aggggataac cttgctaatg gggtgaaggt aggtagtgga
     5401 agcggaaacg ttccatttgt tgttggggaa acaagtcgac taactgcaaa aatcttcgga
     5461 gaccagacat tggttccagg agtctaccgg acaacctttg aattaactac ttggaccgac
     5521 tgatggaaaa ttaacctgat gaaagaaggg ggctatatgc tccctttgtt gatttaccaa
     5581 cataaaatgg ttgtgtttaa atcagcctgg cagtgaaaag tagccatgag attataaatg
     5641 atgctgccca aaagtttaaa gaagccgtta tctttgggtt acattactac ctacaagaga
     5701 aatcttcctc gctaataagg ccctcgctgt acgcttacga agttaatacc gttttgtctg
     5761 tttttttaac aaagaaaata atcctggagt aggttcccgg gcatttgccc gggatgatta
     5821 ctgttttaat gggttattaa tctttgcata ttcaaatgga ctgataaatc tttctctgtt
     5881 tgcatccaga aaatctgccc accactgtag catcagtcga cgttcttcca gatgttctgc
     5941 tttatggata tacgcagcac gtacaccatt acgctcctga tgactgtaag cgtacagcct
     6001 gaaccgtctg gtcagaatct gacgaattag acaaagtggt gtccaccaaa taagtagtgg
     6061 gaaccaaagt atcagatatg cagaaaaatg tgactcccgg caggcgaaaa ggctgcccta
     6121 attatcctcc cgaatttaaa cagcagctcg ttgctgcctc ctgtgaaccc gggatatcca
     6181 tctcaaaact tgctcttgaa aatggcatta acgccaatct gttgttcaaa tggcgacaac
     6241 aatggcgcga gggaaagctg ctattacctt cttcagagag cccccagcta cttcctgtga
     6301 ctctcgatgc agctgccgaa cagccagaat cgctcgcaga ggacccggaa accctcagta
     6361 tcagctgtga ggtaacgttc cggcacggga cgctccgctt caatggcaat gtcagcgaaa
     6421 agctcctgac tctgctgata caggaactga agcgatgatc ccgttacctt ccgggaccaa
     6481 aatttggctg gttgccggta tcaccgatat gagaaatggc ttcaacggcc tggctgcgaa
     6541 agtacaaacg gcgctgaaag acgatcccat gtccggccat gttttcattt tccggggccg
     6601 cagcggcagt caggttaaac tgctgtggtc caccggtgac ggactgtgcc tcctgaccaa
     6661 acggctggag cgtgggcgct tcgcctggcc gtcagcccgt gatggcaaag tgttccttac
     6721 gcaggcgcag ctggcgatgc tgctggaagg tatcgactgg cgacagccta agcggctgct
     6781 gacctccctg accatgctgt aaatctcttt atcctggttg tcacagaata agcccggtaa
     6841 aatacgggct tatgaacgac atctcttctg acgacatctt cctgctgaaa cagcgcctgg
     6901 ccgaacagga agcgctgatc cacgccctgc aggaaaagct gagcaaccgg gagcgcgaaa
     6961 tagaccatct gcaggcgcag ctggataaac tccgccggat gaacttcggc agtcgttccg
     7021 aaaaagtctc ccgccgtatc gcacaaatgg aagccgatct gaaccggctt cagaaagaga
     7081 gcgatacgct gactggtagg gtgtatgacc cggcagtaca gcgtccgttg cgtcagaccc
     7141 gcacccgtaa gccgttccct gaatcactac cccgtgacga aaagcgactg ttgcctgcgg
     7201 cgccgtgctg cccgaactgc ggcggttcac tgagctatct gggcgaggat accgccgaac
     7261 agctggagtt gatgcgtagc gccttccggg ttatccggac ggtacgggaa aaacatgcct
     7321 gtactcagtg cgatgccatc gtgcaggcac ctgcaccttc gcggcccatc gagcggggta
     7381 tcgccggacc ggggctgctg gcccgcgtgc tgacctcgaa gtatgcagag cacaccccgc
     7441 tgtatcgcca gtcagaaata tacggccggc aaggtgtgga gctgagccgt tcactgctgt
     7501 cgggctgggt ggatgcatgc tgccggctgc tgtctccgct ggaagaggcg cttcatggct
     7561 atgtcatgac tgacggcaaa ctccatgccg atgatacccc ggtccaggta ctgctgccgg
     7621 gtaataagaa gacgaagacc gggcggttgt gggcgtatgt tcgtgatgac cgcaatgcag
     7681 ggtcagcgtt ggcacctgca gtgtggttcg cttacagccc ggacagaaac ggcatccatc
     7741 cgcagactca tcttgcctgc ttcagcggtg tgctgcaagc ggatgcgtac gccgggttca
     7801 acgagctgta tcgcaatggt gggataacgg aagctgcctg ctgggctcat gcccgccgaa
     7861 agatccacga tgtgcacgtc cgcatcccgt cagcactgac ggaagaagcc ctggagcaga
     7921 tcggtcagtt gtacgccata gaggcggata taaggggaat gccggcagag cagcggcttg
     7981 ctgaacgtca gcgaaaaacg aaaccgttgt tgaaatccct ggaaagctgg ttgcgtgaaa
     8041 agatgaagac cctgtcgcga cactcagagt tggcgaaggc gttcgcgtac gcacttaacc
     8101 agtggccggc actgacgtac tatgcagatg atggctgggc tgaggcggac aataacatcg
     8161 ctgaaaatgc gttgcggatg gtcagtctgg gccgcaaaaa ctacctgttc ttcggttcgg
     8221 atcatggagg agagcgggga gcgctgctgt acagcctgat cgggacgtgc aaactgaacg
     8281 gagtggagcc agaaagctac ctccgctatg tccttgacgt catagccgac tggccgataa
     8341 accgggtcgg cgaactgctc ccctggcgcg tagcactgcc gactgaataa cacatccccg
     8401 tcaatacggt tcttgctgca cgcttacagg agatgaggcc cgtttttatc gcaatgggtc
     8461 tatggcgctc atgcccgttt gctgttcgat aatggcttct cagagcatac ggtgttgcag
     8521 ttctcgtaaa aactttgcgt cttctggtga taaatccatt gttattactc gtaaagcaat
     8581 gcgcagtaat gccttacgct accgcgctga tggttcttat ccgctttatt gaataggaga
     8641 acaaactgtg agctatcagc ctggtggtct ggttttgcct gatttaagcc gccgtgggta
     8701 tttgtccaag aatgcttcaa gctccaatcg ttttggcctg gtgaatggag atgggttaag
     8761 caaagactct cgttgctggt gtactttttc gcgctggcgc tcaagcatgg ataatgcgat
     8821 tgcctgtagt tcttcgttag tcattgtctg cacctggaaa gcaatttacc ggagtacctc
     8881 acactacggc aaaaatgttt ttattgttgc aacttgcgta attattcagg tcagtttgga
     8941 acgccctgta taaacataaa taacctgata ctgttgtgtg ttttcaaaga aaacacaggt
     9001 atttttcaca taaaccttcc ctttttcata tccgaccact taatcaaaaa acggttttat
     9061 acttgttgcc cccctcaggg gttaaatgtg agacatgtcg cgctttgtgc catgtgtctt
     9121 cactgatttt aggcgcggtt cattcattct ttctgttggc ataaagtatg gtatcggttg
     9181 ggcgaatgaa agggactcgg gtcatagtca gacaatacct gaggcgttaa ttcgttgaaa
     9241 gagattgtga atgggggaat aactgagtct taactgctgt gcgattgccc ggacagataa
     9301 ttgctcgtca aatctgagac gtaatgcgtc tggaaaataa gtgcgaaagc cggtgatatc
     9361 tagtgttgtt tgagagttca tcgttcgagc ccatcaatat agatgggcgc gattgttgcc
     9421 agacaggaca attttcacaa gacgtcgctg atggggcgct tacaagaaaa tgaaatataa
     9481 gagaagagca acgggtgaag aaaagttcaa aaaatggctg cgggtgaccg gatggaaagt
     9541 ggatttgccc ctatatttcc agacacctgt tatcacttaa cccattactg gcccgctgcc
     9601 gcagatattc ccgtggcgag cgataaccca gcgcactatg cggatgccat tcgttataat
     9661 gctcgaacgc ctctgcaagg ttctttgctg ccgttaaccc gtctggtttg ggcatgatac
     9721 ttatgtagtc acgctttatc gttttcacga agctctctgc tattccgtta ctctccggac
     9781 tccgcactgc cgtgttcttc ggttcaagtc ccaacatcct ggcgaactga cgtgtttcat
     9841 tcgcccggta gcatgaacca ttatccgtca gccactccac tggagacgcc ggaagctcgc
     9901 tgccaaagcg gcgttccact gctcccagca tgacgtcctg tactgtttca ctgtcgaagc
     9961 cacccgttgt gaccgcccag tgcagtgcct cacggtcaca gcagtccagc gcgaacgtga
    10021 cccgcagttt ttctccgtta tcacagcgga actcaaaccc gtcagagcac catcgctgat
    10081 tactttcttt cacagccact ctgccggtat gtgcccgttt cgatggcggt acagcgggtt
    10141 ttcgctcaag caacagcgca ttctggcgca tgatccggta aacacgtttg gcattgatcg
    10201 caggcatacc atcaagttcc gtctgtctgc gaagcagcgc ccatacccga cgataaccat
    10261 atgtgggcag ctctccgata acatggtgta tacggcgaag cacatccgta tcatccgtgt
    10321 gacggctgcg gcggccgtcc ttccagtcat cggttcgtct gagaatgacg tgcaactgcg
    10381 cacgcgacac ccggagacaa cggctgacga agcttactcc ccatccccgg gcaataaggg
    10441 cgcgtgcgct atccactttt ttgctcgccc atattcaacg gcttctttaa ggagttcatt
    10501 ttccatcgtt tttttgccga gcaggcgctg gagttcttta atctgcttca tggcggcagc
    10561 aagttcagag gcaggaacaa cctgttctcc ggcggcgaca gcagtaagac ttccttcctg
    10621 gtattgctta cgccagagaa ataactggct ggctgctaca ccatgttgcc gggcaacgag
    10681 ggagaccgtc atccccggtt caaagctctg ctgaacaatt gcgatctttt cctgtgtggt
    10741 acgccgtctg cgtttctccg gccctaagac atcaatcatc tgttctccaa tgactagtct
    10801 aaaaactagt attaagacta tcacttattt aagtgatact ggttgtctgg agattcaggg
    10861 gggcagtcta gaaagagtcc cccctaaagc aggctgacag acatcacaaa tctccggctg
    10921 gttatttgtg tataagagac agctgagctg gaggggacct tttatgcgct atttttatgc
    10981 cgaatccgct aaaacaaaca tcccgtaagg atggatttcg aggtaatagt gctcaccagt
    11041 atcgggctgt aaacgagtgg cgttgacttg caataaaatc tcctgaccat gccattctac
    11101 ggtcacttcg tactgcggcc ccatgtaagc gacatggcga ataacacaac gctgactctc
    11161 ttcgccgtga ttgcttaagg tgatggcttc cgggcgaacg ccgacggtgc ctctaccttg
    11221 caccgggaaa tacgccgcgc gcggcaggcg ataaccgtag atatcgacat actcttcgct
    11281 aaagttcgcc gggaacaggt tggcgtcgcc cataaaactt gccataaagc gggaggcggg
    11341 ctgacgatag agctcctgcg gtgaaccgat ctgcatgatg tgacctttat tcatcaccag
    11401 tacagtgtcg gacaccgcga aagcttcact ttgatcgtga gtcacgtaga gcgacgtgat
    11461 attaaactgc ttttgcagct cgcggatctt atcgcgcata ctgcggcgca ggttggcatc
    11521 gaggttactt aacggctcat caaacagcag cacctttggc ttgaggatca acgcacgggc
    11581 cagagccaca cgctgttgct gaccgccgga aatctgatcg acatagcggt cttcgaatcc
    11641 ttccagatcc accattgcca gcgcatcttt cacccgctgc ttcacttcat tacgcgacac
    11701 gccaagcatt ttcagcccat agccaacgtt ttcgcccagc gacatatgtg ggaacagagc
    11761 gtatgactga aacaccatgc agatatcgcg ttgctgaatg gaacgatgcg tgacatcttc
    11821 gccatctata aaaatttgcc cttcgctcgg tttttccagc ccggcaacca gacgcagaat
    11881 ggtggttttg ccgcatccgg aaggaccgag cagcgtcacc atttgccctt gtgggatggt
    11941 gagattgata ttgtcgataa ccatattact gccgaatcgt ttagtgacgt tgcgcagttc
    12001 cacaaaattt ttctgactca tagtgcgctc cattacgcct ggtttttggc tttagaacgg
    12061 gaaatacgtg cttcaccgat cagccagtca aagaggaaaa taatcgccag catcaccacg
    12121 atcagaatcg aaccgtaggc gatggctacg ccatattcgc cgtcttctac gcggttaagg
    12181 atgtaggcgg ttgccacgcg ggtgtccggc gtgacgagaa agacgatggc gctgaccgtc
    12241 gtgatagccc gcacaaagct gtaaatcagg gccgagagaa tagctgggcg taataacggc
    12301 agtaaaatgt gcgtaatggt tcgcagggaa cctgcacgca ggctaagtga ggcttcatcc
    12361 agcgatttgt cgatctgccc cagacctgca atcccggcgc gaatacccac cggaacgtta
    12421 cgcatcacca tcgagataat cacaattgct gccgttccgg tgagatagac tggggcgcta
    12481 ttaaaggcaa gaatatacga gacacccgcc actgtacccg gtacggcaaa gcacaacatg
    12541 gtggtgaact cgatggtctt tttgcctttg aattgttggc gcaccaccac ccaggcgatg
    12601 agcaggccaa aaatagcggt aatgggtgcg gcaatcccgg cgtagagcaa ggtatctaac
    12661 agtgaaggcc acgcgccgtc gctcatcccc tgaccaaata acttgataaa gttatccagc
    12721 gttagggtgt agtccacgcc ccagttcacg gtgaagctgc cgtagaaaat gctgccatag
    12781 agcagggcgt taaaggcaat ccatatcgca agtatggcga ccacgctaca cactagggtg
    12841 accggtagtg gctggacatc gccgcggtag gactttccgg aaaccgtgac gtaagagcgt
    12901 ttaccaatcc acatatactg gacgcagaac accagtagcg agaacagcag caggaaagcg
    12961 ccaagggtgc tggcggcctg ataatccagt tgcgagccgg taatgtagaa gtaaatttgc
    13021 gtcgccagta cgtcgaagtt gccgccaagc accagcgggt tactgaagtc agccagtgac
    13081 tgcaccacga cgattaaaaa ggcattggca agcgcgggct ttaacaacgg cacaaacaca
    13141 ccgctaaagg tttgccagcg gctggcgcgc agggtgtagg aggcttcttc cagcgaggga
    13201 tgaatggttt taatggcccc atcgaggatc ataaaagcca ttggcgtaaa tgccaggacc
    13261 tgtgccagcc agatcccggt aaaaccatac agccagttgg tgttggtcag cccaaaccac
    13321 tccaccataa attccgttat gtagccggag cgccccatca tgagcgtaac gcctaagccg
    13381 accacaaacg gtggcgtgac aattggcaga atggaaaaga tgcggccgat tacggagctg
    13441 cgtttagcga tgcgcgtggt atatatcgcc agcaccagac cgaagaaagt gcagccaata
    13501 cccaccgcta tcgataagac gatagagttg acgatcacct gaatgatatg ggcctgcgag
    13561 agcacattca taaacgccag tggggcgaac tctcccgcgt cgttggcgaa catcgggata
    13621 aaaatggcga tgctcggcca gacgataaaa atgccaatca gcgccacaat cgcaatcagc
    13681 gagccgatca caaagcggtc gccacccagc cattcgaggc gggttaaggc cagcgtcatt
    13741 atcgcgctta attcgataaa cagcacgatg gcggcgtagc ctagaccgcg gccaacgatg
    13801 gtggcactga tgaccataaa cgctatacaa agcaatgccc agccggcatc aaaataatga
    13861 cggctacgct gctcgcgttt ggcctcatta aacgggcgaa ccagcaacag gctcgggagt
    13921 aaataccaca gccagctgat attaaaatgc gaccagccat aagcctcgag gatctcatca
    13981 ctcgtggact ccagcaggcc ataatcgagg ctccagctcg gcaacagtat aaatgccagc
    14041 cagcccagca aaacccaaag gaaaatcgca tcccgctttt tcacgggatg gagtgcaagt
    14101 gtctgtgaca tagtagttcc ggtatggttg gccggggcag gcgtttgtgc tgcacccggc
    14161 aaatacgcct aatgcgggtt tatttaccca tcttcacttc gctgacccat ttattaatca
    14221 atgccttacg aacatccgtg gaaccatatt tatccatgtc gtagttgatt aatttcatgt
    14281 cgtcgagctt cagcgaattt ggcgacgttt cggcggtggt attggtcagg atctgataag
    14341 acttcccttt cttccacgcc agttcctgag cttcttttga taatacccag tccacaaaca
    14401 gtttggcgtt gtcgaggttg cgcgcgcctt tcagaatgct gacgccgcca atttcgtagc
    14461 ccgtaccttc acacggggaa atcaactcca gcggcgcgcc ctgttctttt tccagcgagt
    14521 agtcatgcag gaagccaata ccaatcgccg tttcgccacg ggcggcgtta cgtgccgggg
    14581 ctatgcctga tttggtgtac tgagagacgt tggcgttcag ttgttttaga taatcaaagg
    14641 cctgatcttc cccccacagc tgcgcaaaag tagccagggc ggtgtaggca gtaccggaac
    14701 tttgcggatc ggcaatttga atttcgcctt tatattccgg tttggtgaga tctttccagc
    14761 acttcggcac cggcaggttt ttctctttca gacgatcggt gtttacgcca aaaccgagga
    14821 tccccacata aacggcagag gacagattcc ctttcacttt tgccggatcg cggaatttct
    14881 ccatgatctg ctcaaggtta ggggatttat acgcctgtag caggcccatc tcgccagcct
    14941 gcgattgcgg gtctaaagtg ccgccgtacc agacgtcagc ctgggggttt ttcttttcag
    15001 catcgacttt tgccagggtg cttccggaac cgttgcggat aaacgaggtt tttacatcat
    15061 atttctcgcc aaacgctttg gtttcggcct cacacatttc attggtggcg ctgcaataga
    15121 ccaccagccg gccttttgcc tgcgcggcac ccgttaaagt cgctagcgca acggcagaag
    15181 caatcagagt agaggtaaag gtcattttca ttgttttatc cttcgagcgt gagtggtggt
    15241 gatgcccgcc atcagtaacg gcatcagcag taaacccata agtactgcgg cgaccgagag
    15301 taaactgaac attcccggcc agccgtagcg ttcgatgacc agcgacagcg gccagcctgc
    15361 cagcgccgcg ccaaggtaag cgaacaggcc gagaaatccg gtgattgaac ccgccgcagc
    15421 tttgtgccca cactccaccg ccgcaagtcc aatcagcatt tgtgggccaa agacgaaaaa
    15481 gcccacggta aaaaagcaca ctgccagcag agcgtagtga tgaaccggtg ccagccacag
    15541 tgcggcgact gacaccatca accccaacgt gaacagcaaa atcattggcg ctcgttgccc
    15601 gctaaacagc aaatccgatc cccatccggc aaataacgcg ccgagtaagc cgcccacttc
    15661 aaacagcatt accgtggcgt tggcgctgag caggttgaca ccgtggcttt ccgtcagcca
    15721 gatattgccc cagtcgttaa gcgcaatccg gatcacataa accagtacat aagagacgcc
    15781 cagtagccag atcagcggat tttgcagcat ggtggtgcgc aacatttgcc acagccccat
    15841 cggcggactt tgttgttcct gacgtaactc cagcggatcg tgacgccagt gaccaactgt
    15901 cggtaatccc tcttcctggg gcgtgccttt gagttgtagc gttaaccaga tccctaattc
    15961 catgctgatt atccccggtg tcagcatcgc ggcctgccag ccccaccagt gggcggcaaa
    16021 ggcgctaatc agcggaataa tcgctccgcc aatgttgatc gacatattcc agcagcccca
    16081 ccagaaaccg cgctcattgc gtgagtacca gtgggtcagc aagcgggcac acggtggcca
    16141 tccccagccc tgaaaaaaac cgttcagtgt ccagaccaca agcagaagcg taagtgattc
    16201 gccaaaggca aacaccacgt tcaataaccc ggtggcgaac aagccgacgc ccataaaacc
    16261 gcgttgtcca tggctgtcat gccacagtcc ggcggtaaat ttcgacaatc cgtagctgag
    16321 ataaaacagc gaacccagca ggccaatgtc gcctttatcc agtccgaggt cggtttgtag
    16381 cgccggaagg acgtaattca cgctttttcg cgtcagataa aatgcggcgt agccaataat
    16441 catgcacatc agcaatcgcg gacgtaatgt ccggtagcat tgattaattt ctctcgctga
    16501 acgcgcgtgc atggttgtct ccatgttgct gactatatga aaatgaaagt gaaaggggat
    16561 gagagaaaat ccgggatacc taagactttt tcctggttat ccggcgattt gttgcaaaaa
    16621 tgtgggcaag ttaacaatta cccgcgtgcc gtgctgtttt tcgagcgtta agtcgccgcc
    16681 cagggcgctg acgcgctcac gcatcccctg aatgccaaat ccggccattt tcccgctggc
    16741 gatccccaca ccgttgtcgc tgacttccag atgcaatacc tctccctgct ggcgcaggat
    16801 aatcgtcact tcgctggctt cggcgtgttt acagatgtta ttgagcaact cctgcaataa
    16861 ccgatacaag gtaaagcgca ccgtttcgtt ttctggtgtg gagttcagtt gataggcaaa
    16921 ctggcaatga atgccgcgtt cactaaaggc aaattcatta atcagatgca gcaacgcttc
    16981 gcgaaatgtc agttcatcaa gcgcgggagg ccgcaactgg cgcagcaatt ggcgggtgga
    17041 gagatgaatg cggcgggcga gctcattgat ttggctggct gcggattgaa tttgcgccgg
    17101 atcacgcgcc cgcttcacca gttgcgactg gatctgaatg gcggtgatgt tctgaccgat
    17161 ttcgtcgtgc agctctcgcg ccaggttttt tcgcgtgtcc tcctcggtgt ggatcagctt
    17221 ttccgccaat gcccgccgcg ctgccagctc ggcctccagc cgttgtcggt aatggtgcag
    17281 gtttagcgcc agatgttgct ggcgactgat ggcaatgccc aggccaattc ccagcaaggc
    17341 ctgggtcgcg aggaaaatct ccagctccac caggttacta aagcccacgc caatctggcg
    17401 ggcgatggtg atcatcatgc tgcccaaaag cccggagagt acgccacctt gccagccaaa
    17461 tttccacgcc atcaccacat tgggcaaaaa gaccacgatc aacagcagac gttcaatttc
    17521 aggtgacagc accatctgag tgccgatacc aataataaaa aacaggctac accagatgat
    17581 taaagaggta cgcaaggggg gattatgcgt atcgagtccc aggagctggt agcgatgctg
    17641 ctggcgcaga aactcaaaga taagatagac aaacggcgtc agtaatacgc cgccggtaaa
    17701 tgaggtcaaa cccggcagca ttattgggcc tgacataaaa ggggagagca gggcggtatt
    17761 caacaatgaa gctgccgtga cagtggctaa cagcaacgtc agacgctgcc agtaaagtgg
    17821 aaaacggtgc cagaagtttt gcgcaaggag gacaggaaac aggctaataa ccggcgttgc
    17881 cagcatgaga taaccgttca atagctgttc actatggagc cagaacatca tcagcaaagg
    17941 ggggagcacc aacgcaggcc agtagcggcg agagagcaaa ataacaaggg cgagataaac
    18001 tccctgcggg agaaacaaca ccgcctgttg accgttattg gtcagataaa agctgagcgt
    18061 ccacagcatc agccagccgg ttccccaggc cagcacaata aacagcgaaa tcacccaatg
    18121 acgaagattg cgcgacatta atgccccgcc agcagatggt ggtcgagcgc aaaatgtacc
    18181 agttcaatgg tgctatggca gttcagtttt cccagcacat tggcgcgatg cacatgtacc
    18241 gttttatgac tgagttcgag tttaaaggcg atctctttaa cgctgtctcc tttgaccagt
    18301 aaatcaaaca cttcacgttc tcgtggggtg agaatttcca gcgcctgggc gggttgcccg
    18361 ccgccgcgta aagcccgaat tgcatcggcg cagagataat gccctcccat accgacagag
    18421 cgcaccgcct gcactaactc ttccggccca cagcgtttgg tcaaatagcc actggcacca
    18481 gcatccagag cgctttgtac aaaggttggc gtgtcataaa tgctaagaat aatggcgcgg
    18541 aattgcggtt tttgtgcacg caggcgctta accaaactga ggccgttttc gtccggcata
    18601 gcaatatcaa tgacggcaac attgacgtca tcacagatta atgccgacca tgcctgtgcg
    18661 gcactactgt actgaccaat gacctccaga tcgtcttcca gagagagtaa ctgtgcgaaa
    18721 ccagagcgca ccaccacatg gtcatccacc agcacaaccc gaatcatact tttcttctct
    18781 tattatctca ttctcctatc atgcatcacg acgcgtcgct ggcaatcaac cagcgacaga
    18841 tatggaatgg atgtaacaaa aaatgtaatt ggttattcaa tataattatg ctttatttca
    18901 tgagtggctc tatatctggt gttcagatta ttgtctgcag gggcagttca gaagatgtga
    18961 gcaaataaaa aagaaaacat aaataaaacc atccggcctc caaagccgga tggttattca
    19021 ttatttctgc atcactgaac tttcattcat caggcctgct cagaaagtga atcaatgtag
    19081 tcagcgtacc attgcatcat ttcacgacga ccatcgagat actgtgcgtg gttgtaggtg
    19141 ccgcgcatga tgtttttatc aacatgggca agctggagtt caatccaggc agagctgaaa
    19201 tcatggtcat ggagaatggt actgaaagta tgccgaaaac catgagctgt agtgaatgta
    19261 tgccagccaa cttgttgaag aaactcgttg atgcaaccgc gaccacgagg ttgattatct
    19321 ctgtatacac caggaataac cagctcggag ttacggggag ctgtgattat ttttatctct
    19381 ttcaataaca tttgagtctg atgagaaagt ggaacaacat gcggccttcg cattttcatt
    19441 ctctcttttg ggattaccca taaggcattg gtaaaatcaa tttcatccca agtagcaagg
    19501 cgtaattctc ccgttctaac agcagttaga accagtattt gcgttaccag agacataaaa
    19561 ttgcctgaaa ctttcatttg gtacatgaaa gcgcctaact gttcttcaga tagcatccgt
    19621 ctctgctgtt tttctggatg aagaacaaca ctgttgagag tcgatgctgg gttaatagtg
    19681 gcgcgccccg tcgcaatggc gtaatcaaat atttgagaac agagctgacg agtcttacgt
    19741 aacgcttcaa gtttgccatt cttttctata accctcagtg cattcaatat ttccaagggg
    19801 cgaatatcag ccacaggacg tttccctagc atgggattaa tatgattctc gaatcttgca
    19861 cgctggtccg ctaaataacc cgcagtccaa cgacaagcca tatgtgtgaa caactcttct
    19921 gcaagagtac aaaatgtgca tgcaatactt tctcttagtg catgtttact cgcctgtttt
    19981 tcatatgaag gatcttttcc ttcagcgagg agtttacgtg cttcatcccg tttctgacgc
    20041 gccagagcca gtgatatacc tggatagaca ccaatcacca atttcttttc tttacccgca
    20101 aatcgatact tcagattcca gtaacgagcg ccagatttct tgataagaag gtaaagtcct
    20161 tgaccatcag aaagtttgta atccttgtca gcaggtttgg ccgactcaat ttgccgaaca
    20221 gtaagtggca tgatgttaac tccgaaaagt agcccccaga gtagccccca aaattatatt
    20281 gatgatcatg gaggatggtt tacgtccaga gtaaaagaat ttatgataat accttatttt
    20341 aaagtgagtt aaagggggtg gagagagttc tgttgaaatc agtggaaata aacagggctt
    20401 aataaatcct ccccgccggg gagtgctggc tggaacggct gcaactggca aaaacgttag
    20461 gcttcgattt tgtcgagatt tcggtagatg aaacggacga tcgcctgtcg cgcctcgact
    20521 ggagccgcga gcagcgtctg gcgctggtga acgcgattgt tgaaaccggc gtgcgcgtgc
    20581 cgtccatgtg cctttctgct catcgtcgtt tcccgctggg cagcgaagat gacgcagtgc
    20641 gggcgcaggg gctggagatt atgcgcaaag ctatccagtt cgcccaggat gtcggtattc
    20701 gcgtgatcca actggcgggc tacgacgttt actatcagga agccaataac gaaacgcgtc
    20761 gtcgtttccg tgacggcctg aaagagagcg ttgagatggc aagccgcgcg caggtgacgc
    20821 tggcgatgga gatcatggat tacccgttga tgaactccat cagcaaggcg ctgggttacg
    20881 cgcactatct taacaacccg tggttccagc tctacccgga tatcggcaac ttgtcggcgt
    20941 gggacaacga cgtgcagatg gagttgcagg ccgggatcgg gcatatcgtc gcggtgcatg
    21001 tgaaagacac caaaccaggc gtcttcaaaa acgtcccgtt tggcgaaggt gtagtggatt
    21061 tcgaacgttg cttcgaaacg ctcaaacaga gtggttattg cgggccgtac ctgattgaga
    21121 tgtggagcga aacggcggaa gacccggcgg cagaagtggc gaaagcgcgt gattgggtga
    21181 aagcgcgcat ggccaaagcc ggcatggtgg aggcggcata atgcaaaagc taaaacagca
    21241 ggtatttgaa gccaatatgg atctgccgcg ctacgggctg gtgaccttta cctggggcaa
    21301 cgtcagcgcc attgaccgcg aacgcgggct ggtggtgatc aagcccagcg gcgttgccta
    21361 cgaaaccatg aaagcagacg atatggtggt ggttgatatg agcggcaagg tggtggaagg
    21421 ggagtatcgt ccgtcttccg acaccgcgac gcatcttgaa ctctaccgcc gttatccgtc
    21481 gcttggcggc attgtccata cccattccac gcatgccacc gcatgggcgc aggcggggct
    21541 ggctatcccg gcattaggca ccacgcacgc tgactacttc tttggcgata tcccctgtac
    21601 gcgcgggtta agcgaagaag aagtgcaggg ggaatacgag ctaaacactg gcaaagtgat
    21661 tatcgaaacg ctgggtgacg ccgagccgct gcatacgccg ggaattgtgg tgtatcagca
    21721 cgggccattc gcctggggga aagatgctca cgatgcggtg cataacgcgg tggtgatgga
    21781 agaagtggcg aaaatggcgt ggattgcccg cggcattaac ccgcaactga atcacatcga
    21841 cagctatctc atgaataaac acttcatgcg taagcacggt cccaacgcct attacgggca
    21901 gaagtaaaac acgcgctgcg gagatttcct tcttcgggag ataactggtc taagtccgca
    21961 gccgtttttc aaaaaaagcc ccctgaaaag ggggcaaagc aaactatggc aatgtttcgt
    22021 tggttatacc tggtgctagc gataaatatc cgcgctggcg tgcatattgc cgttactccc
    22081 cggttcccgc atcagaatca cgtggtagta cgttgcgccc tgcgcatctg tctcttcatt
    22141 taatgcctga cgcgcttcgc tttcagtggc gaaattatga ttgatataaa tcacgcctaa
    22201 gctttgcaca tcgtccatat ttctggcctg gtggttatta atttcaatgg ctgcccatgt
    22261 atttgcactt agcaaaagca cagccagaag ggctaaaaca cggctgaaca tagatacctc
    22321 ctcgacggct gactttgtgt gctctcctct tgtggtgatc ttctgattta attttaatca
    22381 atgataaaga agtggatggt gaccatttct gatgcagttg ttcaaaaaaa caccatgatg
    22441 aagtgtgatg aacttcaaat cagcgtgtta gtacgatttg gtgaaggatg agatttattt
    22501 gtcggctggc cttgagttta gcggggtatg tcagtactat aacgcgtcat ttttcagccg
    22561 acctttaaca cgttccttgc ctccccggga ttcggctgac ccagacagga ggctgaataa
    22621 tccgtaagga gcaattcgat gcgtcattac gaaatcgttt ttatggtcca tcctgaccag
    22681 agcgaacagg ttccgggcat gatcgagcgc tacactgctg ccatcactgg tgcagaaggc
    22741 aagatccacc gtctggaaga ctggggccgc cgtcagctgg cttacccgat caacaaactg
    22801 cacaaagcac actacgttct gatgaacgtt gaagctccgc aggaagtgat cgatgagctg
    22861 gaaactacct tccgcttcaa cgatgccgtt atccgcagca tggttatgcg taccaagcac
    22921 gctgttaccg aagcatctcc gatggttaaa gcgaaagacg agcgccgtga gcgtcgcgat
    22981 gatttcgcaa acgaaaccgc tgatgatgct gaagctgggg attctgaaga gtaatttctg
    23041 atgaccaacc gtctggtgtt gtccggcacc gtgtgcagga ctccccttcg aaaggtcagt
    23101 ccatcaggaa ttcctcactg ccagttcgtg cttgagcatc gttctgtgca ggaggaagcc
    23161 ggctttcacc ggcaggcatg gtgccaaatg cccgttattg ttagcggaca cgaaaaccag
    23221 gccattactc acagtataac ggtcggcagt cgcataaccg ttcaggggtt catttcatgc
    23281 cataaggcaa agaacggact gagcaaaatg gttttgcatg ccgagcagat tgaattgata
    23341 gattctggag actagccata tggcacgtta tttccgtcgt cgcaagttct gccgtttcac
    23401 cgcggaaggc gttcaagaga tcgactataa agatatcgct acgctgaaaa actacatcac
    23461 cgaaagcggt aagattgtcc caagccgtat caccggtacc cgtgcaaaat accagcgtca
    23521 gctggctcgc gctatcaaac gcgctcgcta cctgtccctg ctgccgtaca ctgatcgcca
    23581 tcagtaatcg gtcacggtcc attaatacga ctttgagagg ataaggtaat gcaagttatt
    23641 ctgcttgata aagtagcaaa cctgggtagc ctgggtgatc aggtaaacgt taaagcgggc
    23701 tatgctcgta acttcctggt accgcagggt aaagctgttc cagctaccaa gaaaaacatt
    23761 gaattcttcg aagcacgtcg cgctgaactg gaagctaaac tggctgaagt tctggcagca
    23821 gctaatgctc gcgctgagaa aatcaatgca ctggaaactg ttaccatcgc gtctaaagct
    23881 ggcgacgaag gtaaactgtt cggttccatc ggtactcgcg acatcgctga cgctgtaact
    23941 gcagctggcg ttgaagtggc taagagcgaa gttcgtctgc cgaacggcgt tctgcgtacc
    24001 actggcgaac acgaagtgag cttccaggtt cacagcgaag tattcgcgaa agtgatcgta
    24061 aacgtagtag ctgaataatt cgttattcaa cgagacgtaa aaacgccgac cagtggtcgg
    24121 cgttttgctt ttaagtgata aaaaccgcag aaacagacgt tccctccata aatctcttgc
    24181 tattcctttt ataaaaagtt cgcagtaccc atacctggcc cccagcctcg ccgctggtag
    24241 ttcgtgtcat gttttttacg cgtaaaacac gacaaaagca tcaatacagc gatcgtctca
    24301 ctt
//
LOCUS       DAPHCV010000078        24211 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.078, whole genome shotgun sequence.
ACCESSION   DAPHCV010000078 DAPHCV010000000
VERSION     DAPHCV010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.078"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            529..2028
                     /gene="ansP"
                     /locus_tag="RH033_003101"
     CDS             529..2028
                     /gene="ansP"
                     /locus_tag="RH033_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HDV2992253.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2141..3202)
                     /locus_tag="RH033_003102"
     CDS             complement(2141..3202)
                     /locus_tag="RH033_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HDV2992254.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3444..5546
                     /locus_tag="RH033_003103"
     CDS             3444..5546
                     /locus_tag="RH033_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDV2992255.1"
                     /translation="MKIFSVRQTVFPALLVLSPTVFAADEQTMIVSAAPQVISELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVITQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTEAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMSPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNRGVPEYGQKGELRRDERN
                     LMWNVDPYLQTQWQLTDKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNVYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVKA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGMNIAWRFE"
     gene            complement(5582..6247)
                     /gene="mcbR"
                     /locus_tag="RH033_003104"
     CDS             complement(5582..6247)
                     /gene="mcbR"
                     /locus_tag="RH033_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HDV2992256.1"
                     /translation="MPGTEKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVTLAVE
                     NLTPQDLAELEELLEKLQQAQEKGDMEQIINANRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELRERIENYRLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(6445..7482)
                     /gene="curA"
                     /locus_tag="RH033_003105"
     CDS             complement(6445..7482)
                     /gene="curA"
                     /locus_tag="RH033_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HDV2992257.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDIATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGVEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIQEFQKEMGQWVKEDKIHYREEITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAVDD"
     gene            7663..8178
                     /gene="mddA"
                     /locus_tag="RH033_003106"
     CDS             7663..8178
                     /gene="mddA"
                     /locus_tag="RH033_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HDV2992258.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERSEPDAI"
     gene            8178..8627
                     /locus_tag="RH033_003107"
     CDS             8178..8627
                     /locus_tag="RH033_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDV2992259.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSNVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALIGPICGAILLVVGAWLVARRTF"
     gene            complement(8628..8861)
                     /locus_tag="RH033_003108"
     CDS             complement(8628..8861)
                     /locus_tag="RH033_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HDV2992260.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREERYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(8947..9120)
                     /gene="ortT"
                     /locus_tag="RH033_003109"
     CDS             complement(8947..9120)
                     /gene="ortT"
                     /locus_tag="RH033_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HDV2992261.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            9315..9410
                     /gene="yncL"
                     /locus_tag="RH033_003110"
     CDS             9315..9410
                     /gene="yncL"
                     /locus_tag="RH033_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HDV2992262.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(9507..10931)
                     /gene="patD"
                     /locus_tag="RH033_003111"
     CDS             complement(9507..10931)
                     /gene="patD"
                     /locus_tag="RH033_003111"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HDV2992263.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(10953..11747)
                     /locus_tag="RH033_003112"
     CDS             complement(10953..11747)
                     /locus_tag="RH033_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2992264.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAVYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(11737..12678)
                     /locus_tag="RH033_003113"
     CDS             complement(11737..12678)
                     /locus_tag="RH033_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV2992265.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLIMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            complement(12679..13692)
                     /locus_tag="RH033_003114"
     CDS             complement(12679..13692)
                     /locus_tag="RH033_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV2992266.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSVAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKSASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDALMADKLCGMTGSFALRPEHIRFNIPGEVQANGT
                     IQAVQYQGAATRFELRLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            complement(13710..14855)
                     /locus_tag="RH033_003115"
     CDS             complement(13710..14855)
                     /locus_tag="RH033_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV2992267.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMAKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKALDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRDQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPQGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            complement(15100..16506)
                     /locus_tag="RH033_003116"
     CDS             complement(15100..16506)
                     /locus_tag="RH033_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDV2992268.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGHIIARPQSGYYVATQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLKALELALQEYPVKACWLMTNSQNPLGFTLTPQKKARLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            complement(16585..17001)
                     /gene="hicB"
                     /locus_tag="RH033_003117"
     CDS             complement(16585..17001)
                     /gene="hicB"
                     /locus_tag="RH033_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HDV2992269.1"
                     /translation="MRYPVTLTPAPEGGYVVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            complement(17045..17221)
                     /gene="hicA"
                     /locus_tag="RH033_003118"
     CDS             complement(17045..17221)
                     /gene="hicA"
                     /locus_tag="RH033_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HDV2992270.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIK
                     EPLRKAILKQLGLN"
     gene            17443..17673
                     /locus_tag="RH033_003119"
     CDS             17443..17673
                     /locus_tag="RH033_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HDV2992271.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGHR"
     gene            complement(17765..19726)
                     /gene="rlhA"
                     /locus_tag="RH033_003120"
     CDS             complement(17765..19726)
                     /gene="rlhA"
                     /locus_tag="RH033_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HDV2992272.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPAELHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTIYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(19799..20335)
                     /gene="sutR"
                     /locus_tag="RH033_003121"
     CDS             complement(19799..20335)
                     /gene="sutR"
                     /locus_tag="RH033_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HDV2992273.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQSLNCGEGARFAADVTHIYR
                     NGGEQIVHFHSLIHYPRS"
     gene            20388..21599
                     /gene="ydcO"
                     /locus_tag="RH033_003122"
     CDS             20388..21599
                     /gene="ydcO"
                     /locus_tag="RH033_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HDV2992274.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLALTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLKAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTNDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLATMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALFFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIANRNR"
     gene            complement(21634..22302)
                     /locus_tag="RH033_003123"
     CDS             complement(21634..22302)
                     /locus_tag="RH033_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HDV2992275.1"
                     /translation="MRTTSIAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPVTYYPVPKPSTQVGQKVLDKILSYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(22641..23234)
                     /gene="tehB"
                     /locus_tag="RH033_003124"
     CDS             complement(22641..23234)
                     /gene="tehB"
                     /locus_tag="RH033_003124"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HDV2992276.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLENLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(23231..>24211)
                     /gene="tehA"
                     /locus_tag="RH033_003125"
     CDS             complement(23231..>24211)
                     /gene="tehA"
                     /locus_tag="RH033_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HDV2992277.1"
                     /translation="KVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGLVILA
                     MIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFRPLAV
                     CLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALGYTDA
                     GLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSVNGGE
                     GDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGSGSDN
                     GFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLIRTERAVLMKAEDKE"
BASE COUNT         5936 a         6244 c         5904 g         6127 t
ORIGIN      
        1 tttaagagtt gtaatgacca tggaacagga ctaattccag aaactcctgt tgggcaatag
       61 cgcgtttatc atcctacatg cctcttacat tttttagcct tttctttaac gtcagcaatt
      121 ggctcattta atcagcaatg gtattggtat ttggcgtatt acccctgaaa cccgtcatgt
      181 gctagcacct cactattcaa tgagccaacc acgccagtgt ccactgcaac cactggcgtt
      241 cagagcgacg aacgagtggc gcaagagtag gactgccgtc gggcatcatc aaaccaatag
      301 tggttgtttc cgtcaaaatt tcatcaggtt gctatttggt tgccggccgc ctttatcccc
      361 acatatttat agcgggatta ttttgcatcg ttacacgata aaaggtagga ttctgcgccg
      421 acagttatac ccataaagaa taatggtgaa tcttgtcatc gccaggatga ataaacattg
      481 ttcatggcaa cttatatgac tttttcatta aagcaatcag ggagagcaat gagtaaacac
      541 gacaccgaca cttcagatca acacgccgcg aaacgccgct ggcttaatgc ccacgaagag
      601 gggtatcaca aagcgatggg caatcgccag gtgcagatga tcgccattgg cggcgcgatt
      661 ggtacgggtt tgtttttagg cgcgggagcc cgactgcaaa tggcggggcc agcactggca
      721 ctggtctatt taatctgcgg cttgttttcg ttttttattc tgcgtgcatt gggtgagctg
      781 gtgctacacc gcccttccag tggcagtttt gtctcttatg cccgtgagtt tttgggtgag
      841 aaagccgctt atgttgcagg ctggatgtac ttcatcaact gggcgatgac ggggattgtc
      901 gatattaccg ccgttgcact gtatatgcat tactggggcg catttggtgg tgtaccgcag
      961 tgggtctttg cgctcgctgc gctgaccatc gttggcacca tgaatatgat tggtgtgaaa
     1021 tggtttgccg agatggagtt ctggtttgcg cttattaaag tgctcgccat tgtgaccttt
     1081 ttggtcgtgg gtacagtgtt cctcggtagt ggtcagccgc tggatggcaa caccactggc
     1141 tttcatttaa ttaccgataa cggcggcttc ttcccccacg gtttgctacc tgcgctggtg
     1201 ttgattcagg gcgtagtgtt tgcttttgcc tccattgaaa tggtgggtac agctgccgga
     1261 gaatgtaaag atccgcagac catggtgcct aaagcgatta acagtgtgat ttggcgtatt
     1321 ggcctgtttt acgtcggctc cgtggtgttg ctggttatgt tattgccgtg gagcgcgtat
     1381 caggcggggc aaagtccgtt cgtgacgttt ttctctaaac tgggtgtgcc gtacatcggc
     1441 agcattatga acattgtggt gctgaccgct gccctctcca gcctgaactc aggtctgtac
     1501 tgcaccggac gtattctgcg ctcgatggcg atgggcggtt ccgcaccgag ttttatggcg
     1561 aaaatgagtc gtcagcatgt gccgtatgca gggattctgg cgacactggt tgtgtatgtc
     1621 gtcggcgtat tcctcaacta tctggtgccg tcgcgcgtat ttgagattgt gttgaacttc
     1681 gcgtcgctgg gaatcatcgc ttcatgggcg tttatcatcg tgtgccagat gcgcctgcgt
     1741 aaagcgatta aagaaggcaa agcagcggat gtcagtttta aactgcctgg cgcgcccttc
     1801 acttcctggc tgacattact gtttttgctg agcgtccttg tgctgatggc gttcgattac
     1861 ccgaacggga cttatactat cgcggcgctg ccgattatcg gtattctgct ggttataggc
     1921 tggtttggtg tgcgcaaacg cgttgctgaa attcacagca ctgcgccagt cgtcgaggaa
     1981 gatgaagaaa aacaggaaat tgtgtttaag cctgaaacgg cgagctaatt gcaaaattgc
     2041 ccggctacgt gccgggcaaa ttcaaattac tgacgaaggt gccggatacg gcgtgataaa
     2101 agtcaggcaa gtctctccat tttcaccctc gccatatcta ttacagcgca atacgaatca
     2161 catcgtccgg ctgggtagct tcctgctgtt tagtggattt ttgtttcaca ctgacataca
     2221 gcgttttgcc atcggcagac agtgccaggc tgttcggatg agtcggcgta tcgaacgttt
     2281 tcaccacttt atagcttttc gcgtcaatca cactgacttt acctgcctga cgatgcgtca
     2341 cgtaggcttc attacgcgcg gggttaaaca gcacagccag tgattccggt gccgcaacct
     2401 tcgccagaat attgccatta cgggtatcga ccactaacac ttctgccgct ttagagtcgg
     2461 tgataaatgc acgctgcctt gcggtatcaa ggctgatgtt gataaagaag tgctctttgc
     2521 cgtcatccag cagcttttta cggctgagga ttttattgtc ggcggtgtcg atggtaatca
     2581 attcgccgtc agcgttagtg gtgtaaagac gtttgccttt gctatccagc gctaaaccgg
     2641 tactcatttt accggtgttc tggatggcgg ttttcagttt gatattctcg ccatcaacga
     2701 cccaaatcac gctctcttta ccaataccac tgatgtaaac ggtgttcgtg gcatcgtctg
     2761 ccaccagctc acgcggttgc agcgggcgca cctcttcagt gcgcttacga tcatccagca
     2821 ccagacggcc tttcacttcg cccgttttgg catctatcgc cgtgaccgcg ctgtttacag
     2881 tgttaccaaa ccacaacgtc tgagtcgtgt tattgatggt agcaccaaac ggcttgagat
     2941 cgttatggat cgcctgcgtc acttccagag taaccggatc aagacgataa accacgccgc
     3001 ctttatccag tttgcggctt tgcgaagtgg cgagccacag cgcgttttct tgctggctat
     3061 aagccatttc gtaggcacct ttacctaccg ctttacgcag catttcttct gcggcctgcg
     3121 tactgaatga tgaagcaaca agcaatgaac ctaacagtaa tgaaccacgc aggcgcgatg
     3181 aaaacagatg acgtaaatgc atgacgactc cctttgatga accgatattt ttgctgcttc
     3241 acaatgcttc gctaagcgta atcatcgtta cgctcttgtt ataaatggga atggtaatca
     3301 ttattttctc aaatgtggag catttcttgc cgttacgact acaattttct caattactta
     3361 acatatcgtg ccgtttcgtt ttccataaat ttacaatttt attaccatga cggtgttttc
     3421 ccattcttaa cctggctttt ttcatgaaga ttttttccgt ccgacagacc gtttttcccg
     3481 cgctgcttgt cctttccccc accgtttttg ccgctgatga acagaccatg attgtcagtg
     3541 ccgcgccgca ggtgatttca gaactggata ccccagcagc cgtgagcgtg gtggatggcg
     3601 aggagatgcg cctggcaaca ccgcgcatta acttgtctga atcactgact ggcgtgcctg
     3661 gtttgcaggt acaaaaccgg cagaactatg cgcaagattt acagctgtcg attcgcggat
     3721 ttggctcccg ctccacttac ggaattcgcg gtattcgcct gtatgttgac ggtattcccg
     3781 ctaccatgcc cgacggacaa gggcagacat ccaatatcga tttaagcagt gtgcaaaatg
     3841 tggaagtgct gcgtggcccc ttctctgctc tgtatggcaa cgcgtctggc ggtgtaatga
     3901 atgtcatcac ccagaccgga caacagccac caaccattga agccagtagt tactacggca
     3961 gttttggcag ctggcgctat gggctgaaag caacgggcgc aacgggagac ggcacacagc
     4021 caggcgatgt cgattacacc gtctcaacca cgcgctttac gacccacggc tatcgtgatc
     4081 atagtggcgc acagaaaaat ttagccaatg ccaaactggg cgttcgcatt gatgacgcca
     4141 gcaaattaag cctgattttc aatagtgtgg atatcaaagc agatgaccca ggtgggctaa
     4201 ccgaagcaga atggaaggcg aatccacaac aagcgccccg tgcagaacag tacgacacgc
     4261 gaaaaaccat caagcaaact caggctgggt tgcgctatga acgtagcctg agcgcgcaag
     4321 atgatatgag tgtaatgatg tatgccggag agcgagaaac gacccagtac cagtcaatcc
     4381 ccatgtcccc gcaacttaac ccgtcacatg cgggcggcgt gattaccctg caacgccatt
     4441 accaggggat agacagccgc tggacacacc gtggtgaatt gggcgttcca gtcacgttca
     4501 caaccggcct gaactacgaa aacatgagtg aaaaccgcaa gggttacaat aacttccgcc
     4561 tgaatcgcgg cgtgccggaa tacgggcaaa aaggtgagtt gcgccgcgac gaacgcaatt
     4621 tgatgtggaa cgtcgatccc tatttacaaa cccagtggca actgaccgac aaactttcgc
     4681 tggatgctgg cgtgcgctac agctctgtgt ggtttgattc caatgatcat tacgttactc
     4741 cgggtaacgg cgacgacagc ggtgatgcca gttatcacaa atggctacca gccggatcgt
     4801 taaaatatgc aatgaccgat gcctggaatg tctatctggc agccgggcgc ggttttgaaa
     4861 cgccgacgat taatgagctg tcttatcgcg ctgatgggca aagcggtatg aactttggtt
     4921 taaaaccctc caccaacgat acaattgaga tcggcagtaa aacgcgtatt ggtgatgggc
     4981 tgcttagtct cgcattgttc cagaccgaca ccgatgatga aattgttgtc gatagcagta
     5041 gcggtgggcg tacgacttac aaaaatgccg gaaagacccg tcgtcaaggc gctgaactgg
     5101 catgggatca acgtttcgcg ggagattttc gcgtaaaagc gtcctggacc tggcttgatg
     5161 cgacctatcg cagcaatgtt tgcaatgaac aggattgtaa cggtaaccgg atgccaggga
     5221 tcgcccgtaa tatgggcttt gcgtcgatag gttatgtacc ggaagatggc tggtatgcag
     5281 gtacggaagc gcgttatatg ggcgatatta tggcagatga tgaaaatacg gccaaagcgc
     5341 cgtcttatac tctcgtcggc ttattcaccg ggtataaata caattaccac aatttaactg
     5401 tggatttatt tggtcgtgtc gataatttat tcgataaaga atacgttggc tctgtcattg
     5461 tcaatgagtc aaacgggcga tattacgaac ctgcgcccgg gcgaaattat ggtgtcggca
     5521 tgaatattgc gtggcgattt gagtaattac cttgatgccc ggtatttgcc gggcatttaa
     5581 tttaacgatt gtattgctgg tataaaatag caatattttg ttgcataatt tcagcaagac
     5641 aatgtctgca tccctctttg tcttttgctt ttaacgcggc gagtaataga cgatagtttt
     5701 ctatacgctc ccgtaattcc gctggattaa tcgcttcata gagataatgt aaaccaggtc
     5761 ccatcctgac ccacagttgc tcaatcatct cacacaggat gggcatattt gaacgatgat
     5821 aaatcgctaa gcgaaatagc ctgttcgcat taatgatttg ttccatgtcg cccttttctt
     5881 gcgcctgctg taatttctcg agcaattcct cgagttccgc caggtcttgc ggggtgaggt
     5941 tttcaacagc cagagtaact gccattaatt ccagctcgta gcggatccga ttgatttcgt
     6001 ctaattgacg tttccccact tccggaactg taaatgcttg cgctggtgcg acagaaagcg
     6061 cattcaccga aaccagacgt aataatgctt cacgcacagg tgtaatactc atacctaatt
     6121 gctccgccag atttttagta atcaggcgtg cgccaggttt taatgcgcca atactaagct
     6181 gatgtttcag gtcgttctca acctgcagag tcaaactgac atgtttcatt ttttccgttc
     6241 caggcatttt aattccttcg cgtgttttct gtccaaccct gcctgcatgg cagaaggagt
     6301 tcacctggtg tattaaagtg aataatcata aaccactcac ctttgttgtt attagcagca
     6361 gaacaatgac gtggttagtt aattaaagct gaaaataagc tttttgcctg gatttttatg
     6421 gcggcttacg ccgccgttga aactttaatc atcaaccgcc acgcggatca ccactttgcc
     6481 gaagttttta cccttcagca ggccgataaa cgtctgtggc gcattctcta aaccgtcagt
     6541 aatttcttcg cggtagtgga ttttatcctc tttcacccat tgccccatct ccttctgaaa
     6601 ctcttggatg cggtgaccat aatcctgagc gataataaat ccttgcaagc gaatacgttt
     6661 tttcagcacc gtagccatca gcagaggtaa acgatccgga ccgggtggta gctctgtagc
     6721 gttatagctg ctcactaagc cacaaacggg aatgcgcgca gatgtattaa gtaaaggtag
     6781 cactgcatca aataccttac cgcccacgtt ttcataatag atatcaatac cttttgggca
     6841 cgctttcgcc agttgttcgg caaaatcatc cgcgtgatgg tcaagacaaa catcgaagcc
     6901 cagcacctca gtagcatggc ggcatttttc cacgccaccg gccaccccca ccaccctgca
     6961 acctttaagt ttgccgattt gacccaccgt cgcccccaca ggacctgtcg ccgcagcaac
     7021 aaccaatgtt tcgccctctt tgggctgacc gatatccagc agccccatat aagcggtaaa
     7081 gcctggcatc cccagcacac ccagcgacca cgatggattt tgcggatgat cgccaagttt
     7141 caccagatca tcaccactgg atatgtcata gtcctgccat ccactgtagc ccagcaccca
     7201 gtcgccaggc tggtaatcag gatgattcga ctccacaaca cggctcaccg taccgccgac
     7261 cattacgcca ccaatatcaa ccggcggtga ataagatggc tcatcgctca tacgtccacg
     7321 catatacggg tccagtgaca aataaactgt gcgcagtaac acctgtcctt cacccggtgt
     7381 ggcgatatca tcttcttcaa gacggaaatt ctccggaacc ggcgcgccat gcggacgcga
     7441 ggccagaacc caacgtcgat tacgctgctt ttgttgcccc ataatgctct cctgtgcttt
     7501 tggcatgaat ccatcaatag tagcaggctc gcaggaatgg ttttgtttgc agccgtcaca
     7561 tcccctctgc aacacgcaat aatttcatcg aggaatccgt tgcttcgggc tgttttcgat
     7621 agtattgata acgtcatcat cattatttaa tcgaggccaa acatgtccat ccgttttgcc
     7681 cgcaaagccg actgtgctgc cattgcggaa atttataacc acgccgtgtt gtatacggcg
     7741 gctatctgga atgaccaaac ggtggatgca gataaccgca ttgcctggtt tgaagcgcgg
     7801 accatagcag gttatccagt gctggtgagc gaagaagatg gcgtagtgac gggatatgcc
     7861 tcgtttggcg actggcgtag tttcgatggt tttcgccata ccgtggaaca ttcggtttat
     7921 gtccatcccg atcatcaggg caaaggtctg gggcgtaaat tgttaagccg attgattgat
     7981 gaagcgcggg attgcgggaa gcatgtcatg gtcgccggga tcgaatcgca aaatcaggcg
     8041 tctttgcatc tgcaccagtc gctgggattt gtcgtcaccg cgcaaatgcc gcaggtgggc
     8101 actaaatttg ggcgctggct ggacctgaca tttatgcagt tgcaactcga cgaacgcagt
     8161 gaaccggacg caatctgatg aatcagtcgc tcacccttgc ttttttgatt gccgccggga
     8221 ttggtctggt agtccagaac acgctgatgg tgcgcatcac ccagacctcc tctaccattc
     8281 tcatcgccat gttgctgaac tctttggtag gaattgtgct ctttgtttct attttatggt
     8341 ttaaacaagg tatggcgggg tttggcgaac tggtgtccaa cgtgcgctgg tggacactta
     8401 tacctggctt gctgggatcg ttttttgtct tcgccagtat cagcggatat cagaatgtgg
     8461 gagcggcgac aactattgcg gtactggtcg ccagccagct gattggcggt ctggtgctgg
     8521 atatcttccg cagccatggc gtaccactac gggcgttgat cggcccaatt tgtggcgcaa
     8581 ttttacttgt ggttggtgcc tggctggtgg ccaggcgcac attttaatca gaggatggtg
     8641 ccacctttgg ttaactgttc gtggcgcgct tccatatctt ctttatgttt gcgaccgtga
     8701 tgggcgatcg ctgtgcgaag acgctgctgc tgggtatagc gttcttcccg acttaactcc
     8761 gcatcatcgc tcagggcgat taataattcg ttcatatggg caatgacgct ctcttcaatc
     8821 gcggcatcca cgcgcgcgat gacttcatcc agatgcgaca tactttctcc ttagccactt
     8881 gtgaaattta tcgattcatc caaatgatat aagtctgcgt ggtgccgtca agccagtgag
     8941 taagatttaa aacagtgaaa acaacagcgc caccgggaaa ctcatcggcc atgttgcccc
     9001 caccagaaag gcgcttaaga aacggatgcg tttcttatcg tgagaaagaa accaggtgat
     9061 aagaaatgcg attgctgcca taaccgcata aaaaacaagc atgtgttgat agagagacat
     9121 gtacgggcgt ccagccagat gaataaaacg cgcgcaaata tgcgtcattt tttgacgtac
     9181 atcaagtttt ttaaccggtt tgagaaaaaa acatcaaaca gcaacttgcc cttatccatc
     9241 ttcagaatga gtattattgt ggattttctt aacccgtact atacacatag gggcaatgat
     9301 aaaaggtggc aaaaatgaat gtttccagta gaactgtagt actgataaat gtctttgctg
     9361 ctgttggttt gtttactctt atctctatga gatttggctg gtttatttga tgtagtgatt
     9421 ttgcacgcag cacgtactgt cagagaaatc agtgctctga cagtacgcaa gatcatctca
     9481 tactcttacg atctgcaata ttatccttaa tgtttaacca tgacgtggcg gacgacggtg
     9541 taatcctcca gcccataaag tgacatatcc ttgccgtaac cagaaagttt ctgcccaccg
     9601 tgcggcattt cacttaccag catgaaatgg gtattgaccc aggtacaacc atattgcagc
     9661 cgtgcgctga cgcgatgcgc cctgcccaca tctttcgtcc ataccgaaga tgcaagtccg
     9721 tactggctgt cattcgccca gtttaccacc tgttcttcgt tgtcgaagag tgtaacactc
     9781 actactggac caaatacctc tttttgcacg atggcatcgt cctgtaatgc gccagccagc
     9841 agcgtcggcg catagtaata gccattaccc ttgcgctttt caccgccagt gatcactttg
     9901 atgtgccctg tcgctttcgc ctcttctact gccttgctga cgcgttcgag atgcgccagc
     9961 gagcttaaag gtccaagctc cgtagactcg tcatccggcg caccagattt taacgttgcc
    10021 accgcagcac ccagtttttc caccagcgta tcgtaaatgc ctttttgcgc gtagatccga
    10081 caagccgctg tacaatcctg tccggcattg taatagccaa atgtacgtac accttcgacc
    10141 actgcctcaa tatccgcatc atcaaaaaca atcactggcg ctttgccacc aagttccata
    10201 tgagtacgct ttatggacgg cgcggtatgg ctgatgatgt gttccccagt ggcgatagag
    10261 cccgtcagcg acaccatccg cactttggga tgaccggtca gcggatcgcc caccgttttg
    10321 cctctgccaa acagtacatt aatcacgcca gccgggaaga tatctttcgc caactctgcc
    10381 aacttcaacg ctgtcagcgg ggtaatttct gatggtttaa gcactacgca gttccctgcc
    10441 gccagcgccg gagccagttt ccacgcagcc atcatcagcg gataattcca cggtgcgata
    10501 gaagccacga cccccaacgg atcgcgacgg atcatcgaag tatgaccttc aagatattca
    10561 cctgccgcca gaccattcag acagcgcgcc gcaccagcga aaaagcgaaa aacatcgaca
    10621 atcgccggga tttcatcatt gaacgcacta tgcagcggtt tgccacaatt acgggactcc
    10681 agttcggcaa aaacctgacc attttcttcg ataacatcag ccagtttcag cagacattcc
    10741 gcacgcactt tcggcgtggt ttgcccccat tcggcaaatg ctgcatctgc cgcgcgaaca
    10801 gcagcatcga cctgctctgc ggatgcctcg gcaatctcca gtaaaacgtc ccccgttgcc
    10861 ggattataga caggctgttt ttccccttcg ccgctaacca gttctccgtt aatcagtaac
    10921 ttatgttgca tagcatattc ctgtatcagt ggttattgac cattgtcgcc ttcgcgggtt
    10981 agccaccagg cccccaggat cggcaaggtt gttaccaaca taaccagcag tgccaccacg
    11041 ttagttaccg gtacatcacg cggtcgccca agctgattga gcaaccacaa cggtaacgtt
    11101 cgttcatgac ctgccgtaaa ggtcgtaacg atgatttcat cgaacgacaa ggcaaacgcc
    11161 agcattcctc ctgccagtaa cgccgaactg agattcggca acactacgta gcggaaggtt
    11221 tgccagccat tagccccaag atccattgac gcctcaacca gactccacga ggtgcggcga
    11281 aaacgggcga tgacattgtt aaagaccacc actacacaaa aagtcgcatg accgaccacg
    11341 atggtgaaaa atcccggctc cagattgatg gttttaaagg cggttaataa cgccagacca
    11401 gtgacaatgc ccggcagcgc aatgggcagc agcagtaaca gcgaaatggc gtttttgccg
    11461 aaaaagtctc gtcgccacag cgcggcagcg gccagcgtcc ctaacaccag cgcgattaat
    11521 gtcgccagcg ccgccacttt aagtgacagt gtcacggcat caagaatatc actacgctgt
    11581 gctgccacgc taaaccagcg cagcgtcagg ccttgcggtg gaaaactaaa cgccgcatct
    11641 tcagtgttaa acgcataaac ggcgataatc agaatgggaa aatgtaagaa aaccacgccg
    11701 ccccaggccg ccagtttgag gaaaaacggt gcgcgttcag agtgcatcga acgctcccag
    11761 acgtttcacg aacgccaggt aaagtgcgat gagtacaatc ggcactaggg tgaatgccgc
    11821 cgccatcggc atattgccaa tcgccccctg ctgggaataa accatattgc cgataaaata
    11881 tcctggaggg ccaaccagct gcgggacaat aaaatcgccc agcgtgagtg agaaggtaaa
    11941 gatagagcca gcggcaatac ccgggattgc cagcggcagc accacatagc gaaaggtttg
    12001 tcgtggacgt gcgccgagat cagctgacgc ctgcaacaat gacggcggca aacgctcaag
    12061 cgccgcctgc accgggagga tcatgaacgg caaccagata tagagaaaca ccagaaagcg
    12121 ccccagcccg gaagttgaca gcgtattacc tcccaccgcc ggtagcgtga ggaacgcagt
    12181 cagcagtggt tccagcccca gatgttgtaa aaaccactgc gccacgccat cttttgccag
    12241 caataacgtc caggcatagg ctttcacaat gtagctcgcc cacatcggca acattaccgc
    12301 gatataaaaa aacgctttca ttttcccgct ggtatagcgc gccatatacc acgccattgg
    12361 aaaagccaga atggcgctgg cgatagtgac tgccacagcc ataatcagcg tgcggagaat
    12421 gatgtcgtaa ttcgccggat taaacagcgc acggatattc gccagcgtca gttccggcgt
    12481 taccgacatg gtgaaatcgt caaaggtata aaagccctgc cataacagcg ttagcagcga
    12541 gccgaaatag acaatgccaa accacattag cgggccaagc agcagcaaaa acagccccag
    12601 ccccggatta cgccagaaga aaccggacac cttacccaga cctggacgtg aaggtgattg
    12661 caatacgttc atcgccattc acctctcctc aaccagcggt accatcacat cacgcgacca
    12721 ggaaaccatc acctgttgtc cgggcgtgag cgtggcgggc agttcttcgc ctgtcatatt
    12781 ggcctgactc accagcagtt tttcaccgcc gttcagtctc agttcaaaac gggtcgccgc
    12841 gccctgatac tgcaccgcct ggatcgtgcc attagcctgc acttcgccgg ggatgttgaa
    12901 gcggatatgt tccggtcgca gggcgaagct tcccgtcatg ccacaaagtt tgtctgccat
    12961 cagtgcatca aaaacattcg atgtcccgac aaacccggca acaaacggcg tgcgcgggcg
    13021 catatagaga tcacgcgggg aatcgacctg ctcaatgcgt ccattattga aaaccgccac
    13081 acgatcggac atcgataacg cttcgccctg atcgtgggta acgaagataa aagtgatacc
    13141 gagagactgt tgcagctttt tcagctcaag ctgcatttgc tctcgcaatt tgagatccag
    13201 cgcgccgagc ggttcatcca gcaacaacac acgcggttcg ttcaccagtg ctctggcgat
    13261 agcaacccgc tggcgctgac caccagaaag ttgtgacggt ttacgttgct gaacaaaacc
    13321 aagcgccact ttctcaagcg cctcttgcgc cattgcgtgc cgctgctttt tattcacgcc
    13381 tttgaccatc agcccatagg ctacgttgtc aagaatcgac atatgcggaa atagcgcgta
    13441 gtcctggaag acagtattca cgtcccgctc ccacggtggc agattgctgg cggatttacc
    13501 aaagatagag atagccccgc cggaaagctg ttcgaagcca gcaatcaggc gcaggcaggt
    13561 ggttttgccg gagccggacg gccccagcat agagaagaac tcaccatctt ttatcgcaac
    13621 actgacgcca tctactgcgc gcacgtcacc gtacaaccgt gagacgttgt caaactccac
    13681 tgcgtacgtc ataaaacacc ccagcgaagt tagcgaccgc ccataatggc aatgtaatcc
    13741 tgcgtccagc gactgtaggg aacaaacttg cccccttctg ctataggcgt tttccagaag
    13801 gcgattttat cgaaatagtt aaaaccgttt gtttcacaac ctttctcgcc taataacgga
    13861 ctggctttac acccttgcgg cactactggt aacgagccaa accaggccgc cacatcgccc
    13921 tgcacttttg gggttaatga ccagttcatc catttgtagg cgcaaaccgg atgtttcgct
    13981 tcgctatgta gcatggtggt gtcagcccaa ccggtaacgc cctccttcgg gaaaacggta
    14041 gcgacaggct ggccttcggc tttcagggcg ttggcctgat aaggccatgc actggaagca
    14101 accacaccct cgtttttgaa atcgctcatt tgcacggtag tgtcatgcca gtagcgatgg
    14161 atcaaactat gttgatcgcg cagcactttc agcaccgcct ggtactgttc ttcggtgagt
    14221 tgatacggat cgctgatgcc caactgcggc tgcgtggctt taacgaacaa cgcagcgtcc
    14281 gcaatgtaga tagggccatc ataagcctgt acccggcctt tattgctctt gccgtccggc
    14341 agattctgct caacaaaaac cacttgccag ctatccggcg gcgtcgggaa ggttttagtg
    14401 ttgtacatca gcaggttcgg cccccattgg taaggtgtgc cgtaaacttt gccgccaacg
    14461 ttaaaccagt cgcctttaac cacgcgcgga tcgagcgctt tccagttggg aatcaaggcg
    14521 gtattaattg gctgcacgcg tttacccata atcaaacgca gcgaggcatc gccggatgcc
    14581 gtaaccagat cgtaaccccc tttggccatc agactgacca tttcatcgga agtcgcggcg
    14641 gttttaacat tcaccgcgca gcctgtctct ttttcgaatt gcgttaccca gtcgtattgt
    14701 ttatcagttt gtccgcgttc gatgtatccc ggccaggcga taatatccag tcgcccttcc
    14761 ggtttatcta aattggtagg tggttcggcg gcgtgagcgg tcattattgt catggtgagc
    14821 gcacacaggc tgctgcgggc aaatgtcttg ctcataaagt cttactcctg tcgtaattaa
    14881 ttgttcagcc gccgtgccgt aaaaatatct ccttgtttaa ccatagacgg cggctagcag
    14941 gcacaccttc gcccctgaga aaatttaatc aggttgtgag ctaccgcgca ttatgccgta
    15001 tgtaaggtta tcttctggag tatagacaag gagttaggaa atatgactgc tattcgaaat
    15061 tcatattacc caacctgaga ggaatttaat taaacgctat tacagccgtt cttgaataag
    15121 tttgcctaat tgtttaaccg cctgttcttc acgttctccc cactgccacg cggtattaaa
    15181 acggaaaaaa cgtgaccagt tttcaccggt agaaaacatt ttacccggcg caatactgat
    15241 atgatgcgtc agtgctgcca ggcttaattc gcccgcatct aacggctcgg ggagctccag
    15301 ccagagaaaa taaccgctgt cattatgatg aattttcact tccgcaggca gataacgcag
    15361 cagtgcttgc caggcacgtt gtttacgttc cgcaagctgg cgacgcaggc gacgaagatg
    15421 ggcgtcgtat cggcgcgtgg aaaggtaatc caccagcgca agctgcatcg gtgagctggt
    15481 ggaaagcgta ctcatcaact gcaagcgttg aattttacgt gcatgttttc cggcggcgac
    15541 ccaaccaata cgaaaaccag gcaccagaca tttcgaaaac gaagagcaat gcaaaacacc
    15601 atcgtggcga tcccacgctt tcgcaggcag cggtttttcc cgtccaaaat aaagttcgct
    15661 gtaaacgtca tcttcaatca gcgttacgtt gtactgattg agcaacgcta ccagccgcgc
    15721 ttttttctgc ggcgttaagg taaatccgag tggattctgg ctattagtca tcagccagca
    15781 cgctttcacc ggatactcct gcaacgccag ttccagcgcc ttgagatcaa tcccttcttt
    15841 aacgtccgtc gccaccgata acgccttcag ccgtaaccgc tccagcgcct gcaacgcacc
    15901 gtagaaacaa ggattctcta ctatcaccca atcgcccggt tcagtgaccg cttgtaaact
    15961 gaggtttaat gcctctaacg ccccggcagt aatgacaatt tcatcgggtg aaatggtgat
    16021 gccctgtaag gcatagcgac gagctatagc ctgacgcagt tcagcgtttc ctggcggtaa
    16081 gttttcaatc acgctcatcg ccgtggcggt tttgcttacc tgtgccagcg agcggttgag
    16141 ttgttggagg gggaaaagtc gcgggtcggg aaaggccgag gcaaacggaa ctaccgacgg
    16201 atcgcgactg gcctgcaaca tatcaaaaat ataggtgttg atatcgactg cttcatctcg
    16261 agtgactgga atgactggcg ctttcggcat ttttattgcc tgtgtcgcaa cgtaataacc
    16321 cgactgcggt cgcgcgataa tatgtccctg actttcgagc aactgatagg catggctgac
    16381 agtcataaag ctcatgccag aaagcgccac ctggtcacgc aacgaaggca aacgatcgcc
    16441 aggttgccag atacccgacg caatctgctc gcgtaattgt tctgcaagct gctggtattt
    16501 tttcattatc gaatcgtaat tatgtgcaga tgattcggca gtctatatca gttgttaaaa
    16561 atgacaactt ttcattaact gttatcaaac catcaccagt gataactctt tgccaagcgc
    16621 cttagccgca agctggacgg cgtcgatttt tgtagcatgg tgcaagttaa ataagcgagt
    16681 aatctcctgt ttaggtttgc caattcgtct ggctaactct tgctgagtga tttctgactg
    16741 taaaaaagca tttaacagca atactttaga ggcgatactt aaaggcactt caataaagtg
    16801 atcgttgtta gttaatggcg aaggcaacgg gattagctca ttatcttcaa aataaaaatc
    16861 aaatgcggtg agtaaggcat cttttgctgc ttccatcgct tcagcaacag tttctccctg
    16921 cgtcaacgct tcagggatat ctacaaaaga aaccacataa ccgccttccg gcgcaggagt
    16981 aagagtgacg ggataacgca taattttgac tgtctctcgc atgtcattaa ccttcttatg
    17041 gcgattaatt taaaccgagt tgcttcagga ttgctttacg caatggttct tttatctcat
    17101 cactggggtg acgcggcatg acactgcgtc gcccttgaaa cctgagtttc aaatggttgc
    17161 tgccattcgc cacatcgacg ccctgagatt cgagccaacg tctgaactcg ctttgtttca
    17221 catcgcctcc ctgccgctga gtatatcaac aaagtaaaca ttagtgttta cctccgtgtg
    17281 taatttgctc gaaaatagca ctaaatcgcc gcaacggcag aatgcaatgc aaaatcagga
    17341 aggaagattc cagaatttct ttgcgctaat ttgcaaaact gaataataac ctcaagaaac
    17401 gatcggcatc atagcgacaa aaggtaagaa aaaggtggca ggatgtttac gaaggcgtta
    17461 tcagttgtct tattaacgtg tgcgctgttc tcaggacaac tcatggcagg gcacaaagga
    17521 catgaatttg tgtgggtaaa gaatgtggat catcagctgc gtcatgaagc ggacagcgat
    17581 gaattgcgtg ctgtggcgga agagtcggcg gaaggtttgc gcgagcattt ttactggcaa
    17641 aaatcgcgca aaccagaagc gggacatcgt tgagtcattt gttgccggat gcggcatgac
    17701 gccttacccg gcaggcaata tcatgcagat ttgatgagtt gtgagtaacc agaaaactgg
    17761 cgttttaccc ttttcgcttc ggtaaagttt tcagcagctc atccggactt acggatgcca
    17821 ctacgcttcc cggtaacggc attttcagta tgtgattttt gattttgcca atgacgtgca
    17881 tctcgcatgg gcggcaatca aactttagcg ttaatacttc atcgccgtta accagttgca
    17941 ttggcgtcgc cttccagctt ttgatattgc ctttcgcctg tttcgggcac agattgaagg
    18001 caaagcgcag acaatgctta gtgatcatca ccgggacttc gcccttctct tcatgcgctt
    18061 catacgccgc atcaatcagt tgcacaccat agcgatgata aaattcacgc gctttctggt
    18121 tgtaaacgtt cgcgaggaaa ctcaaatgcg tttgcggata aaccggcgca ggatcagcaa
    18181 ccggtttacg gctgccgcgc tggtaactgg caagacgcgc agcatctagc atgtcggcgg
    18241 cttcacggcg gaactggttt aacagactgt ttggtacaaa cagcgcaccc ggcaaattaa
    18301 tttgcacatc gcgggcgtaa tagatggttt gccccagttt tgccagacca tcctttagat
    18361 tgttcattgc tttttcggcg ttattggctt cgtcgaactg cccgtccagc gtatgcgtga
    18421 tgctgacacc ctcttcactg gtgagggtca gaatcagttg ttcctgccag ccgcccagtt
    18481 caatgtctac cgccacccga cgttcgctgg aagtttttgt cagtgcctgc tgccagttat
    18541 gatcaagatt acggtttagt gggtgatgcg ggcgaatttt gtgcaactct gctggcattt
    18601 cattgggcca gacgcggtac tgattttctc cggttttctc gaccgtattg gcacgaaagc
    18661 cgacgacttc acgtttaatc atcacgttca ggccatcgcc atttgccagt ggctcggtaa
    18721 cggcaacatc gagatgatct ttcgccactt tcaatacttc acccaccggc aggccgataa
    18781 atttcggcga atcgaacgcg ccaatatcgc ctttacgggc attcacaaaa taatctgtgc
    18841 taccacggtg gaaagtcttt tccgtcgatg gaacaaagaa atgttcagta cgacctgatg
    18901 aagcacgcgt cagatcgcca cgttcttcaa taatggcatc cagcatctgg cgataatggg
    18961 cggtgatatt cttcacgtag ctcatatctt tgtaacgccc ttcaatcttg aaggagcgta
    19021 cgccagcatc aatcagcgcg ccgaggttgg cagtctgatc gttatctttc atcgacagca
    19081 gatgtttttc ataggaaacc acccgcccct gatcgtcttt caatgtgtat ggcaaacggc
    19141 acgcctgcga gcaatcgcca cggttggcgc tacgccctgt ttgcgcatga gaaatgtagc
    19201 actgacccga ataggccacg cacagtgcgc catgaataaa gaattcaatg gtcgcgtccg
    19261 tggcctggtg aatcgcgcgg atctgctcaa gattcagctc tcgcgccagc acaatctgcg
    19321 tgaagccaac atcagagagg aacttcgctt tttctactgt acgaatgtcg cactgcgtac
    19381 tggcgtgcag ttcaatcggc ggaatatcaa gttccagaat ccccatatcc tgaacaatca
    19441 gcgcgtcgac gccggtctgg tagaggtcgg taatcagccg ttgcgcaggt tccagctcat
    19501 catcatgcaa aatggtgtta agcgtgacga aaatttttgc accataacga tgggcaaatg
    19561 gcaccagttc ggcaatatct ttcaggctat tactggcatt atgacgggca ccaaaaccag
    19621 ggccgccgat ataaacagca tcggcaccgt gcaaaatagc ttcgcgggca atggcggcat
    19681 cgcgtgccgg gcttaacagt tcaagtcgat gagaagatac ggtcacattc ttatccagta
    19741 gctgaaaaat ggcggctatt ttagccacaa gcgcgacgtt gcgaaatagt tttcccgctt
    19801 aactgcgtgg gtaatggatg agggaatgaa aatgtacgat ttgctcccca ccattacgat
    19861 agatatgcgt aacgtctgca gcaaatcgag cgccttctcc acaattaagg ctctgccatt
    19921 caccatcaac gcacagatca agttgcccat caatgacgac aacatgttcg attactccct
    19981 tctcatgggg cgtcgattca cttattgcac caggtgccat ctgaatcgag aagtgttcaa
    20041 agcagagttg tggatcataa ggaaacagcg aagtgatcac catcgcctgc tgttgaggat
    20101 cataaactga aggcgtagcg gactgcggcg gagaaataaa tgtcgaaaat ggcacattca
    20161 acccggtggc aattttccat aatgtcgcga ccgtcgggct ggactcatta cgctcaatct
    20221 gccctaacat cgctttagaa acgcccgtcg cttctgccag tcgcgaaaga ctccagccgc
    20281 gctgctgacg tagttgttta agtgtggtgg ataaaaagcg agcgagattt tccatgcgac
    20341 tcctccggca aaacgtaagt ttatcacttg tgcgttataa cggacaaatg ctacggtgcc
    20401 tgtatgctat aacgcacgag gtgactatgc gtctgttttc tattcctccg cccacgctac
    20461 tggcggggtt tctggcggta ttaattggct acgccagttc agcggcaata atctggcaag
    20521 cagcgattgt cgccggagcc accactgcac aaatctctgg ctggatgacg gcgctggggc
    20581 tggcaatggg cgtcagtacg ctggctctga cattatggta tcgcgtacct gttctcaccg
    20641 catggtcaac gcctggcgcg gctttgttgg tcaccggatt acagggacta acacttaacg
    20701 aagccatcgg cgtttttatt gtcaccaacg cgttaatagt cctctgcggc ataacgggac
    20761 tctttgctcg tctgatgcgc attattccgc actcgcttgc ggcggcaatg cttgccggga
    20821 ttttattacg ctttggttta aaggcgtttg cgagcctgga tggtcaattt acgttgtgtg
    20881 gcagtatgtt gctggtatgg ctggcaacca aggccgttgc gccgcgctat gcggtaattg
    20941 ccgcaatgat tattgggatc gtcatcgtta tcgcacaagg tgacgttgtc acaaatgatg
    21001 ttgtctttaa acccgttctc cccacttata ttacccctga tttttcgttt gctcacagcc
    21061 tgagcgttgc actccccctt tttctggcga cgatggcatc gcaaaacgca ccgggtatcg
    21121 cagcaatgaa agcagctgga tattcggctc ctgtttcgcc attaattgtg tttactggat
    21181 tgctggcact gtttttttcc cctttcggcg tttattccgt cggtattgcg gcaatcaccg
    21241 cggctatttg ccaaagcccg gaagcgcatc cggataaaga tcaacgttgg ctggccgctg
    21301 ccgttgccgg cattttttat ttgatcgcag gtctgtttgg tagtgccatt accgggatga
    21361 tggctgccct gcccgtaagt tggatccaga tgctggcagg tcttgcgctg ttgagtacca
    21421 ttggcggcag tttgtatcag gcgctgcata atgagcgtga gcgcgacgcg gcggtggtgg
    21481 catttctggt aacggcaagt ggattgacgc tggtcgggat tggttctgcg ttctggggat
    21541 taattgccgg aggcgtttgt tacgtggtgt tgaatttaat cgctaacaga aaccgataat
    21601 aaaaagcgcc atctaacgat ggcgcaactg gtattacttt ttgttaacgt caaacatggt
    21661 ggcatcggtc gccatgtcat caataacttg tttaatattt tcgaaagcca ttggcgtact
    21721 ttcgttattc aggtctttac cttcgccctg acgaacgact ttgataaccg gtttattagt
    21781 cgctgcatca atcagctcac cttcaaaata caggcgagta tccatggtac ggtggcccgt
    21841 cgccatttgc gtccccgcaa ccactaatgc aacaggaacc acttcataga attgcagccc
    21901 ttctttgctg gtatctacac cggtaatggc cccacggaaa atcagactac gcggcccagc
    21961 ggtggtaacc agtggtttac gctgcgcaat cgcttctttc atttcggtgt tggtataact
    22021 caaaatttta tccagaactt tctgccctac ctgggtagat ggtttcggta ccggataata
    22081 agtgactggg ttccagacga tgctgtcata tttgctttga tcaaaactcg ggtctaccca
    22141 acgtaaaaca ggtttacctg ttgctgaggt tgtttctttt aaatcagagt aattgtttaa
    22201 aaaaccagaa tatttatctg gctgggtgat tttagaggca caaccagaca gagccaacaa
    22261 gccgcataaa gctgcaactt tcgcaattga tgtggtacgc atgaatagtt tcctgagatg
    22321 attatattac tttctaagtt atagcaaaaa gcgccgcttc gtgttgtggc aaaaaaagtg
    22381 ttggtgaaaa taaatttttt agcccctttt cagacgacat agtcaatgcc gaaaaagaca
    22441 tagtcaatgc cgaaaaaggg gctgccgaaa aaggggctaa gtcaatcatc agtagttaaa
    22501 tttcagtaag ttaagctgct aaaaaatttc gactactgtg attaatttca catccattcg
    22561 ataaaaaata tgcttaccga aaagaagaac agtaaaccat ctcagcgtgg ggaattttca
    22621 cgcgccttgt tcctagcggg tcatttttta cgtgccagca tcgtggcgaa acgcagttta
    22681 atacgattac cgttggcgtc ggtgcggtgc agctcgccga cgtcttcatt gtatttcacc
    22741 atctcccagc cttcgtaata tcgacgtaat tccccctctt tgaaggcaaa cgggaagccg
    22801 acggtacatg gataatcagc ggtatccatc gccgccacaa tcaggttgta accgccaggt
    22861 ttagtgcaac gttgcatatt ggcaatcagc cctgggatgg ttttagcctc aaggaacatc
    22921 agcaccacag tcgaaagaat aaaatcatac tgtccatcaa atgtgaggtt attcagatca
    22981 acgacccggg tgtgtaaatt ctccagattt tcaatggatt taatccgctc gacgttggcg
    23041 atactcatgg catttttatc ccatgcgtca acatcataac cattggctgc caggtaaaga
    23101 ctgttacgac cattgccaca gcccagatcc agcgttttac ccggtttaac cactttcacc
    23161 gcttccagca cttcagagtg tgtgcgagtt aattcatatt tatcagtaaa atagttttcg
    23221 tcacgaatga tcattctttg tcctctgctt tcattaaaac ggcgcgctcg gttctgatta
    23281 acaattttcc ctgcatcaga agcgcaaaag tacggatgag cagtattgca ataataaaat
    23341 tggtaaagat aaacagcggc accgccagcg tatggaaaaa tccattatcg ctgccactcc
    23401 ccagatgcaa accggtggtt gccagtgcag atacgccgaa cgagaaactc cagaatgaag
    23461 cattaaatgg ctgggagaga taccatggca tcagacgtag cataaacagc agttgcagca
    23521 gtccataacc gaaaagcatt ttcgccagcg tgtcaccctc gccgccgttg acgctcagcc
    23581 aggcactaca ggccaccagc gcaggagcga gctgaatgcc gagtgatgtc cgcagcgccg
    23641 tgggtaattc tcccgaactg cgcagacgct gcaagatcac aggttcaagg cttagccatg
    23701 agaaaacgcc tgcgcctaaa aacaccagac cggcgtcggt gtagcccaac gcaccacagg
    23761 ccattgcgct gataaagttg ttggcaactg tcggcagata cagtccaggc gtggtagctt
    23821 cttcagggtg agatccgcgc cataatcccg cagtttgcca ggcggcataa gccaactgaa
    23881 ccacgacgcc aaaactgaac aggcacaccg ccagtgggcg aaaccacgga acaaaaccaa
    23941 tcgccaccag catcgtcgtt gccggaaaca aactcacaaa actgctcagc actggatggc
    24001 gcacttccgc cagcacgcta tgcggaaagc gtatgagtcg ggtaataaat gcgctagtta
    24061 ataatcccca gatgatcatc gccagaatca ccagcccatc ccctaaccag tggctgacct
    24121 gccaaacctg gctggcatag cgccaggcaa atcccatccc tatcgtcccc aacacaatac
    24181 caaagtagcc tgccggcaaa ttgagcactt t
//
LOCUS       DAPHCV010000079        24136 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.079, whole genome shotgun sequence.
ACCESSION   DAPHCV010000079 DAPHCV010000000
VERSION     DAPHCV010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.079"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            242..1138
                     /locus_tag="RH033_003126"
     CDS             242..1138
                     /locus_tag="RH033_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDV2992278.1"
                     /translation="MADSTRNRSAERLIDILVELQNYGVVSRHNLMKKYNITERTAYR
                     DLNMLSPFIEACGDGKYRLISARAGNQSKESLHKSLARLLDTDAIFPERDEGFWQKLE
                     NRATEKHIRVQFHNPEHTIRDDLRKYLDVLEKAICNSNVCQIAYAGKTRIVHPYKLTN
                     QRSIWYLLATEENKLKSFSLAKIKWLDIKKEKFAKSDEIQSLVSESCDPWVSDKTFDV
                     VLFIYSNIAHYFLRRDLLPYQQLLHKHDNGITLSCKAAHKNQIVPLILYWLPNVEIIE
                     PVWLKEAVLTMLGKYLTAENVS"
     gene            1187..2266
                     /locus_tag="RH033_003127"
     CDS             1187..2266
                     /locus_tag="RH033_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HDV2992279.1"
                     /translation="MALPFIILGVAAGAAAFGGKKAYDGYQKKSEAEDILEKAKERYE
                     EHKEKFEQAEAQTSSKLTTLGNKELEIGKDFSEFDRIAAELLARLEKEGNKDLKLSVP
                     QHNINKIQNLAISATEYLSTVVAAGVSGAAAGFAVYSGVMAFAAASTGTPIAALSGAA
                     AYNATMAAIGGGSLAAGGMGMAGGAMVLGTAVAAPLIAIAGWAYDRHATKALENAHQC
                     ACDVARAVKKMNLAREHFAKVNQYVDEILAALNRMHAVFIKHYFEPLKSMHSVITEQQ
                     DISVSDEVIVLIDHGYSLAAIMTDIITTPLFKPRKQENGEAVIKDNVIEMETDDNGMN
                     VINKDELDDVLASSVVKFDDFSSQS"
     gene            2313..3884
                     /locus_tag="RH033_003128"
     CDS             2313..3884
                     /locus_tag="RH033_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP--cob(I)alamin adenosyltransferase"
                     /protein_id="HDV2992280.1"
                     /translation="MNVEKFDWTEELHQTMVKSLVTSFGLDFLLLNDRKGGDVDTIHN
                     VRNGIYATEAERQRYEGRGEYDSHHYHSHENYIATNREGKRAHQAGTLTDAYTGEVFA
                     SDDKKNLDHIISAKEIHDDPGRILAERDGAELANDASNLAFTHESLNKSKKADSMDAF
                     ICTLQKNREDTLRQIAELESQATLTEKEKKRLISLRKKADADTERMERADKYAREKYE
                     ATINQSYYLSSKFATAVASASLNSGFRMGTRQMLGLILAEIWFELRERIPHIIRQQRN
                     SFSAGKCLSEITSALRACQERVQEKFSSFLIAFKDGVIGGILSGITTTLLNIFLTTER
                     MIVRLIREMWNNLVQAFKVLVFNPEGLSPGQLAKAVTKLIAAGVAVAGGVMINEAMAQ
                     ILIFPFGSELAAFCGALTTGILGLVMNYYLEYSEVMQRVWAFLDKFKDKFQRSLEYFQ
                     AVNAELDRYLVELTALEFSVDAAELNHFSLHLDSVNSEIERGLLLREEVRRRGIALPF
                     ESGNVASVRDWLNKR"
     gene            3881..4147
                     /locus_tag="RH033_003129"
     CDS             3881..4147
                     /locus_tag="RH033_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDV2992281.1"
                     /translation="MKLHPPFPCNQCGACCRHVNRAGETQCLDRGDGICRHYQTDSHL
                     CAIYDKRPQICRVEDQYLLNYQSQYSWQEFIALNQAACLILNKL"
     gene            4287..4484
                     /locus_tag="RH033_003130"
     CDS             4287..4484
                     /locus_tag="RH033_003130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992282.1"
                     /translation="MRLLLKGLVGMALAWLVIGFGMELFFLADRVHPALRVIVVLGML
                     FLLIMIAGGMCRYLLSRKKTS"
     gene            4550..6223
                     /locus_tag="RH033_003131"
     CDS             4550..6223
                     /locus_tag="RH033_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDV2992283.1"
                     /translation="MYSSATVCFTVSAGQKYRFYSIIMKINLPFIHWSRISNDKRLVL
                     SVVVFTLSNILVSSGAHLIYRILFFTLALLIIKAMKSLTLRFILAFSFTLLTAADISI
                     SLYSWCTFGTTFNDGFAISILQTDPDEVIRMFRMYVVYVIAFIILFLLFFCSAINKTS
                     SLPLEKVTVITFLLLITVTLYSSFQFALKKQYQINEVDPYIVASRFATYTPFFNLNYF
                     ALAAKEHQRLMTIADTIPHYDLVITDNNTDVFVLVIGESARADNMSIYGYSRPTTPEL
                     QKQKSRLKLFTQAISGAPYTALAVPLALSVNTVLHHDVRHYPDNIINMANQAGFDTWW
                     LSAQSAFRQNGTAVASIAMRARNRIYVRGYDELLLPHLAEALNSNPGGRKLIVLHLTG
                     SHEPVCSNWPRDKAVFKPLDTEEVCYDNSIHYTDSLLGQVFAMLETRRASVMYFSDHG
                     LEYDPTKEHAYFHGGIKPSQQAYHVPMFIWYSPALGEHVDRQTVNSVFSTAYNDYLIN
                     AWMGVTRPAQPKTPEEVITRWQGKSVVFDASHNVFDYKVLRKNFNEPLE"
     gene            complement(6367..7410)
                     /locus_tag="RH033_003132"
     CDS             complement(6367..7410)
                     /locus_tag="RH033_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDV2992284.1"
                     /translation="MNDRLRFEVNDNQGRFVFPDIWFGPLLGEFEELLDAYDTDEISE
                     TSYINKLRRLAQQEPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRLIPESFSG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKKHADEFSPYWYELGLLHFLNGELVKAATAFRRGFAANTYIAEIL
                     CGNLHPFPLAVWHNYSAGPDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERAE
                     IIKCAEMLMQEDDFEICESILRQQKLLRERIDETLSEEIVQKCRNINGEYVWPWILPF
                     SAAGMKHTSIQHR"
     gene            complement(7844..9034)
                     /locus_tag="RH033_003133"
     CDS             complement(7844..9034)
                     /locus_tag="RH033_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDV2992285.1"
                     /translation="MALTARQVETARPKEKDYKLSDERGLYLLVKTTGARYWRLKYRI
                     AGKEKKLALGVYPDVSLAEARIKRDDARKIISEGGDPGEKKRKEKLTQKISATNTFHA
                     LATEWHQHKSLSWSESYARSVLEALDKDIFPYLGKRSVTDILPLEMLEILRRIEKRGS
                     LEKLRKVRQYCNQIFRYAIATGRATVNPASELTSTLAAPKAAHFPHLRADELPVFLRK
                     LAEYHGSPVTRMATNLLLLTGLRTIELRSAEWSEIDFDNALWTIPESRMKMRRKHVVP
                     LSRQATDILLQLKTFSGQYRLVFPGRCDINKPMSEASINMVLKRIGYDGRATGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNAIRGTYNHAQYLDGRREMMQWYADYIDSLSRQ
                     ESQG"
     gene            complement(9211..9285)
                     /locus_tag="RH033_003134"
     tRNA            complement(9211..9285)
                     /locus_tag="RH033_003134"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9250..9252),aa:Phe,seq:gaa)
     gene            complement(9391..10098)
                     /locus_tag="RH033_003135"
     CDS             complement(9391..10098)
                     /locus_tag="RH033_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDV2992286.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVTKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            10490..12625
                     /gene="speC"
                     /locus_tag="RH033_003136"
     CDS             10490..12625
                     /gene="speC"
                     /locus_tag="RH033_003136"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HDV2992287.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHVVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVVDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVEIGIEARKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(12671..13927)
                     /gene="nupG"
                     /locus_tag="RH033_003137"
     CDS             complement(12671..13927)
                     /gene="nupG"
                     /locus_tag="RH033_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HDV2992288.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAEVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(14129..15208)
                     /gene="mltC"
                     /locus_tag="RH033_003138"
     CDS             complement(14129..15208)
                     /gene="mltC"
                     /locus_tag="RH033_003138"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HDV2992289.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(15273..15548)
                     /locus_tag="RH033_003139"
     CDS             complement(15273..15548)
                     /locus_tag="RH033_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HDV2992290.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(15576..16628)
                     /gene="mutY"
                     /locus_tag="RH033_003140"
     CDS             complement(15576..16628)
                     /gene="mutY"
                     /locus_tag="RH033_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDV2992291.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPALGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIATANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHDDEVLLAQRPPSGLWGGLYCFPQFADEESLRLWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEDNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            16789..17508
                     /gene="trmB"
                     /locus_tag="RH033_003141"
     CDS             16789..17508
                     /gene="trmB"
                     /locus_tag="RH033_003141"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDV2992292.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            17508..17834
                     /locus_tag="RH033_003142"
     CDS             17508..17834
                     /locus_tag="RH033_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HDV2992293.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLGEVRTSEL
                     FDVWWD"
     gene            18018..18737
                     /locus_tag="RH033_003143"
     CDS             18018..18737
                     /locus_tag="RH033_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HDV2992294.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            18913..19959
                     /gene="ansB"
                     /locus_tag="RH033_003144"
     CDS             18913..19959
                     /gene="ansB"
                     /locus_tag="RH033_003144"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HDV2992295.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            20076..21083
                     /locus_tag="RH033_003145"
     CDS             20076..21083
                     /locus_tag="RH033_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HDV2992296.1"
                     /translation="MKKQWIVGTALLMLMTGNVRADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     NQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(21139..22440)
                     /locus_tag="RH033_003146"
     CDS             complement(21139..22440)
                     /locus_tag="RH033_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HDV2992297.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIAIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRDLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLIALMMITYIPQISLFIPQLLGLM"
     gene            complement(22440..22943)
                     /locus_tag="RH033_003147"
     CDS             complement(22440..22943)
                     /locus_tag="RH033_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDV2992298.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
     gene            complement(22988..23974)
                     /locus_tag="RH033_003148"
     CDS             complement(22988..23974)
                     /locus_tag="RH033_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDV2992299.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSKGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQQQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
BASE COUNT         6481 a         5435 c         6024 g         6196 t
ORIGIN      
        1 tgtcagtctg ttaaatgaaa aaggactgtt gttgggacac gttgtttttt gctatatcag
       61 ctcttccgga aacaataaac cttattacga aagaggcacc tgggatatcc ggtttaaagt
      121 ttgattataa tcaaataaaa atttgttgcc ggagggcgta gttttgtgga actgacaaat
      181 gatgtcagga gtatttatat aattcactta cacgagagta atactacagg tgcagtgatt
      241 tatggctgac tcaacgagaa atcgctcagc ggagcgtttg attgatatcc ttgttgaatt
      301 acagaattat ggtgtggtca gtcgccataa tttgatgaaa aaatacaaca tcactgagcg
      361 aacagcatat cgtgatctga atatgctttc tccctttatt gaagcctgtg gcgatggaaa
      421 ataccggctt atttcagcaa gagcaggaaa ccagagcaaa gaatcactgc acaaatctct
      481 ggccaggtta ctggatactg atgcgatttt tcctgaacgg gatgaaggtt tctggcagaa
      541 attagaaaac agggcaaccg aaaagcatat tcgtgttcag tttcataacc ctgagcacac
      601 tatcagggat gatttaagaa aatatcttga tgttcttgaa aaagcgatct gcaacagtaa
      661 tgtttgccag attgcatatg ccggtaagac cagaatcgtt catccctata aattaactaa
      721 tcagagaagt atatggtatt tgctggctac tgaagagaac aagttaaagt cgttttcatt
      781 agctaaaata aaatggctgg atataaaaaa agaaaagttt gcaaagagtg atgaaataca
      841 gtcactagta agtgagtcat gtgacccgtg ggtgagcgac aaaacatttg atgttgtgtt
      901 gttcatttac agtaatatcg cccattattt tttgcgacgg gacttacttc cttaccagca
      961 attgctacac aaacatgaca atggcattac gctgtcatgc aaggctgcac ataaaaatca
     1021 aattgtcccg ttgattttat actggctacc gaatgttgaa attattgaac cagtatggct
     1081 gaaagaagcc gtgttaacaa tgttaggaaa ataccttact gctgagaatg tttcctgaaa
     1141 tatttcagtt gactgatgat aaagcaatct gtacaggagg aaaagaatgg cgttaccttt
     1201 tattattttg ggtgtggctg ccggcgctgc ggcattcggc ggaaaaaaag cttacgatgg
     1261 atatcaaaaa aagtcagaag cagaggatat cctcgaaaaa gcaaaagagc gttacgagga
     1321 gcataaagaa aaatttgagc aggctgaagc tcagacatca tcaaaattaa caacgctcgg
     1381 aaataaagag cttgagattg gtaaagattt cagtgagttc gacaggattg ctgctgagct
     1441 cctggcgcgt ctggaaaaag agggcaataa agacctgaaa ctgagtgtcc cgcaacataa
     1501 catcaataag attcagaacc tggccatttc agcgacagaa tatctcagta cggtcgttgc
     1561 tgccggagtt tccggggctg ctgcgggttt tgctgtgtat agcggagtga tggcatttgc
     1621 tgcggcttct acaggtacac ccattgcagc gctttctggt gccgcggctt ataacgcgac
     1681 gatggcggca attggagggg gctctctcgc ggcagggggc atgggaatgg ccggcggcgc
     1741 aatggtatta ggcaccgcag tggctgcacc gttgattgcc attgcgggct gggcttacga
     1801 cagacatgcg acgaaagctc ttgaaaatgc ccatcagtgt gcctgcgatg ttgcacgggc
     1861 agttaagaaa atgaatctgg cccgggagca ttttgcaaaa gttaatcaat acgttgatga
     1921 aattctcgct gcactgaaca gaatgcatgc tgtatttatt aaacactatt ttgagccgct
     1981 taaatccatg cattctgtta ttacagaaca acaggatatc agtgtatcag atgaagtcat
     2041 tgtgcttatt gaccatggat attcactggc cgctattatg acggatatta ttacaacgcc
     2101 actgtttaaa ccgcggaaac aggaaaatgg tgaggcagtg ataaaagata acgttattga
     2161 aatggaaacg gatgataatg gaatgaacgt tattaataag gatgagctgg atgatgtact
     2221 ggccagttcg gttgtgaaat ttgatgattt ttcatcccag agttaatgat ttaccgggcg
     2281 ttaaagcccg gctttacaag aagtgaaata acatgaatgt ggaaaaattt gactggacag
     2341 aagagttaca tcagaccatg gtgaagagcc tggttacatc attcgggctg gattttctgt
     2401 tgctaaatga tcgtaagggt ggtgatgttg acactatcca taatgtgcgt aatggcattt
     2461 atgccacgga ggctgagcga cagcggtatg aaggtcgtgg cgaatatgat tctcatcatt
     2521 accacagcca tgagaactat atcgcgacga accgggaagg aaagcgtgcg catcaggcag
     2581 ggactcttac tgatgcatat acaggcgagg tgtttgcttc tgacgacaaa aaaaatctgg
     2641 atcatattat ttccgctaaa gaaattcatg atgatccggg gagaattctt gctgaacggg
     2701 atggtgcaga actggcaaat gacgcttcaa atctggcatt cactcatgag tcgctcaata
     2761 aatcaaagaa agcagactca atggatgcgt ttatttgcac gctgcagaaa aacagagaag
     2821 atacgttaag gcagatagca gagcttgaat ctcaggcaac gctgacagaa aaggaaaaga
     2881 aacgtctgat aagtctgaga aaaaaagctg atgcagatac tgagcgtatg gaaagggctg
     2941 ataaatatgc ccgggaaaaa tatgaggcca cgattaatca gagctattac ctgagtagta
     3001 agtttgctac tgctgtcgcc agtgcttcgc ttaattccgg ttttcgtatg gggacgcgac
     3061 agatgttggg gcttattctt gccgagatct ggttcgaact gagggaacga atcccgcata
     3121 ttatcaggca gcaacggaac tcattcagcg ccggcaaatg cctgtccgaa ataacgtcag
     3181 cattacgtgc ctgccaggaa cgagtacagg aaaaattcag ttctttttta attgccttta
     3241 aagatggtgt catcggaggg atactgtcag gtattaccac aacgctgtta aatatattcc
     3301 tgacaacgga aagaatgatt gtccggctta tcagggaaat gtggaacaat ctcgttcagg
     3361 cctttaaagt cctggtattt aatccggagg gattatcgcc ggggcagtta gccaaagcgg
     3421 tcactaaact gatagcagca ggtgtggctg ttgccggagg ggtgatgata aatgaggcga
     3481 tggcgcagat ccttattttc ccatttggtt cagagcttgc tgccttttgt ggtgcgctga
     3541 cgacaggtat tctcgggctg gtgatgaatt attatctcga atatagcgag gttatgcagc
     3601 gggtatgggc atttctggac aaatttaagg acaaattcca gcggtcactg gaatactttc
     3661 aggcagtcaa tgctgaactt gatcgctatc ttgtggaact tactgcactg gaattttctg
     3721 ttgatgctgc cgagttaaat catttttcac ttcatctgga tagcgtaaac agtgagatag
     3781 agcgcggact tctgctgaga gaagaagtcc gccgacgagg gattgcgttg ccgtttgagt
     3841 caggaaatgt ggcttctgtt cgtgactggt taaataagcg atgaaattac accctccatt
     3901 tccctgtaac cagtgtgggg cctgttgtcg tcatgtcaac agggcgggtg aaacgcaatg
     3961 tctggatcgt ggtgacggta tttgccgtca ctatcagact gattctcatt tgtgtgcaat
     4021 ttatgataaa cgcccacaga tttgccgggt tgaagaccag tatttactga attatcagtc
     4081 tcagtacagc tggcaggagt ttattgcatt aaaccaggcc gcatgcctta ttctgaataa
     4141 actgtaatgc cattaatggc ggcgtcagga cagaaactga gagaacataa aacagtaaaa
     4201 tgtcatacca ttcagcagcc agcgttactt tccccggctg ctgttattgt aaggcatttt
     4261 cgttaagaat acagaaggag aggagcgtga gattgctgtt aaaagggctc gtcggaatgg
     4321 cgctggcctg gcttgttatt ggtttcggga tggagttatt ttttctcgcc gatcgggttc
     4381 atccggcatt aagagtaatt gttgtcctgg ggatgttatt cctgctgata atgattgcgg
     4441 gtggtatgtg ccgttatctt ctgtcacgaa agaaaacatc atgacgatcc tgtttacgcc
     4501 gctacatttg tggcggcgga tgatgcccgt tttatgatta ttcagaaaaa tgtattcatc
     4561 tgccacggta tgtttcacgg tcagcgccgg gcagaaatat cgcttttact caattattat
     4621 gaaaattaat cttccgttca tacactggtc acggattagc aatgataagc gcctcgtgtt
     4681 atcagtggtt gtctttaccc tttctaatat ccttgtatcc tcaggagcgc atctgattta
     4741 ccggatatta ttcttcaccc tggcgcttct tattattaag gcgatgaaat cgcttacgct
     4801 gcgttttatt cttgcctttt cgttcacatt gctgacagcg gcagatatca gtatcagttt
     4861 gtattcatgg tgtacattcg gtacaacatt taatgatggt tttgcaatca gtatccttca
     4921 gacagatcct gatgaagtaa taagaatgtt caggatgtat gttgtttatg ttatcgcatt
     4981 tattatcctc tttttattgt tcttctgttc agcaataaat aaaacgtcct cattgccatt
     5041 ggaaaaggtc acggtaatta catttttatt gttaattaca gtaacgttat attcttcctt
     5101 ccagtttgcg ttgaagaaac agtatcaaat aaatgaagtt gatccttaca tagtcgcttc
     5161 ccggtttgcc acatataccc ctttctttaa tctgaactat tttgcactgg ccgcaaagga
     5221 gcatcagaga ttaatgacga ttgctgatac catacctcac tacgatctgg tgattacgga
     5281 taacaacact gacgtatttg ttctggtgat tggtgaatct gcaagggctg ataacatgtc
     5341 catttacggg tattcgcgcc caaccacacc agagcttcag aaacagaaat cacgactgaa
     5401 actgttcact caggcaatca gcggggcgcc ttataccgcc cttgcggtac cgctggcatt
     5461 atctgtaaat accgtgctgc atcatgatgt tcgccattat cccgataata ttatcaacat
     5521 ggcgaatcag gcgggattcg atacctggtg gctcagcgca caatcggcgt tccggcagaa
     5581 cggaacagct gtggccagta ttgccatgcg ggccagaaac agaatttatg tcagaggcta
     5641 tgatgagtta ctgctcccac acctggctga agcactgaac agtaatccgg gaggcaggaa
     5701 actgattgtt ctgcatctga cgggcagcca tgaaccggtt tgcagtaact ggcccagaga
     5761 taaagccgtg tttaaaccgc tggatacaga agaagtctgt tacgataatt ccattcatta
     5821 taccgacagt ttgttggggc aggttttcgc catgctggaa acgcgccggg catccgtcat
     5881 gtatttttca gatcacggtc tggagtatga cccgacaaaa gaacatgcct attttcatgg
     5941 tggaataaaa cccagccagc aggcttatca tgttccgatg tttatctggt acagcccggc
     6001 gctgggagag catgtcgatc gccagacggt gaacagcgtt ttttccactg cgtataatga
     6061 ttatctgatt aatgcatgga tgggcgtaac cagaccggct caaccgaaaa cgccggagga
     6121 ggtgattacc cgctggcagg gaaaatccgt ggtttttgat gccagccata acgtgtttga
     6181 ctacaaagta ttacgaaaga actttaatga acccctggaa taattcgtta aagtaattta
     6241 ctacgctatt ttttcaaagg aaactttcag aaaataaccc cagggcgata acctggggaa
     6301 gtggggggct gtgttattac tggtggataa tttattactt gtttgaaaga tacactaatg
     6361 caatatctac cgatgctgga tgcttgtgtg tttcattcct gccgctgaaa acggcaatat
     6421 ccagggccag acatattcac cgttaatatt tctgcatttc tgtacaattt cctcagaaag
     6481 tgtctcatca attctttccc gcaacagctt ctgctgtctt aaaatgcttt cgcatatttc
     6541 gaaatcatct tcctgcatca gcatttcggc gcatttaatg atttctgccc gttcatgcag
     6601 cacagaggaa tggttataga gccagtttac aaacagcaat gcttccggat attgtcccca
     6661 taatggtgag tatgttgcat aataatcttc ggcagtatcc ggcccggcag aatagttatg
     6721 ccatacagca agaggaaatg gatgcagatt tccgcacaga atttctgcga tgtaagtatt
     6781 tgccgcaaat ccgcgacgaa atgcagttgc agcttttacc agttccccat taagaaaatg
     6841 cagaagacca agttcatacc agtaaggtga aaattcatca gcatgttttt tcagtacact
     6901 gaaggctcgc tcatgatcac ctgttcgtaa cagctctgaa cccaacaacc agcgggcacc
     6961 ctgattatct tcaggattgt atgccagtat tttatcagta agcataaccg catcctgatg
     7021 acgctgtaag tgcacatttg cgagaattgc tgcataaagc gcgcgcagat aaggccggtt
     7081 ttcgggatgc atccagatga tttcaccact gaaactttcc gggatgagac gattacctgc
     7141 cgccagtcct ttaagagcgg cgttcagtgc cttacgcggg gcattctgct ccagaaatgc
     7201 atatgccaga tgagcgtgta tatcaataaa gtcaggctcc tgttgagcaa ggcgtctgag
     7261 tttgttgatg taactcgtct cgctgatttc atcagtgtcg tatgcatcga gcagttcttc
     7321 aaactccccc agtaatgggc caaaccagat atccggaaat acgaaacgcc cctggttatc
     7381 gttcacttca aagcgtaatc tgtcgttcat tttctgagcc tgtaaaacgg atgggacaca
     7441 atctgaaaag cataaaagtt gtgatgttgt gtatcaagtc ttgtttttgt ccgggatgaa
     7501 tgttgtttac aatatcatcg gcagtggttt ttcatgagaa tgaaaattta tctttatctg
     7561 cattccggtg tattgtgaat tgtcggctga aacagcatat taatcgattt ttttagggga
     7621 atggtgctga aataacgaca tgatgatgat ttatattaca ggtatcaaaa ttgttagcgg
     7681 tccataacgg tgtgatgttt tgagattgtt tacagatgca atattaaata ttttgtcaaa
     7741 aaacaaaaaa tatctaaaaa taaagcatga aaacaaaatt cctccggacc tggaggccgg
     7801 aggagaactt gtggattgag agaaaatatc agaacaagtg ttcttaaccc tgactctctt
     7861 gcctggaaag cgaatcaatg taatctgcgt accattgcat catttcacgg cgaccatcga
     7921 gatattgggc atgattatag gtacccctga tggcgttttt atccacatga gctaactgca
     7981 tttcaatcca ggcagaatta aagccctgtt cgtgcagaat ggtactcatg gtgtgacgaa
     8041 aaccatgacc ggttgccctg ccatcgtaac cgatacgttt gagcaccatg ttgatgctgg
     8101 cttcgctcat tggcttgttg atatcacaac gtcccgggaa aaccagccgg tattgtccgg
     8161 agaaagtttt gagctgcagc agaatgtcag tggcctgtcg tgacagtggt acgacatgtt
     8221 tacgtcgcat tttcatgcgg ctttcaggga ttgtccacag ggcattatca aaatcaattt
     8281 ctgaccattc agcggaccgt agttcaatcg ttctgaggcc tgtcagaagc agcagatttg
     8341 tcgccatgcg ggtaacagga ctgccatgat attcagcgag cttccggaga aaaacaggga
     8401 gctcatctgc tctcaggtgg gggaaatgtg cagcttttgg cgccgccagc gtactggtca
     8461 gttcagatgc cggattgaca gtggctcgtc cggtggcgat ggcataacga aaaatctgat
     8521 tacagtattg acgcacctta cgaagttttt ccagcgagcc acgtttttct atgcggcgca
     8581 gaatttccag catttccagc gggaggatat cagtaacgct tcgtttgccc agatacggga
     8641 aaatatcttt atccagagcc tccagtacgc ttctggcgta actttcagac catgacaaag
     8701 atttatgctg atgccattcc gtagcaaggg catggaacgt attggtggca gagattttct
     8761 gagtgagttt ttcctttcgc ttcttttcgc ccgggtcacc accttcggag atgatttttc
     8821 gggcatcgtc gcgtttgatt ctggcctcag caagggagac gtcgggatag acgccgaggg
     8881 ccagtttttt ctcttttcct gctatccggt atttaagccg ccagtagcgg gcacccgtgg
     8941 tttttaccag cagatataaa ccacgttcgt cagagagttt atagtctttt tctttaggtc
     9001 tggctgtttc gacctgtctg gctgtaagtg ccatatgggg gctcctgaaa ctgactgaac
     9061 acaatgagcc cccaccgggg cccccaatga acgcttgatt gagagagagt tcagtagagt
     9121 ctggtggagt tcagaatgtt cagattgctt gtaacgatgt gattatactt gagtttcgtg
     9181 gagttcggga gatgaatctg gaagtgaaag tggtgccgga ctcggaatcg aaccaaggac
     9241 acggggattt tcaatcccct gctctaccga ctgagctatc cgggcaacgg ggcgcattaa
     9301 acctgattcg cctcgtctcg tcaatcaaat ttcttcaatt tactgcagac tgcacaatct
     9361 atcatcactt tgccgctatt gcacgcattg tcaggcaaac caggcggtcc aggcggcgga
     9421 aagcggcatt gccagaagga gtgctggaag catgttgacc accgggaaca ttttaatgcc
     9481 acagatgcgt aatccggttg ccagcagcaa taaaccgcct acggcgctga agtctgccat
     9541 catcgacggt gtggtcagcg gtaatatcag cgcggcagcc cacgccagcg ttaactggat
     9601 gatcagtaat gggatactaa ttaccgacac cgcaataccc aacgagcagg cgaagatcat
     9661 cgccgtaaag aaatcgagaa atgacttggc gattaaaata ctcggatcgc cggtcatccc
     9721 ttcgttcatc gccccgaaga tcccggtgcc gctggcgcaa aacaggacaa taatcgcgac
     9781 ataattctga ataaaagatt catgcgctgg cttcttacgt gagtggcgaa acagattttg
     9841 tgctttggtg accgcggtat tgacgccttt ttccagcaga caaatttcgc cgattaatgc
     9901 accgagtaag gttgctaaaa ccatcgccgg aaggttggcg catttcacca ccagtaaaat
     9961 accaatcccc agcgatgcca gaccaaaaat agatgtcatg gagacgcgga tacgttccgg
    10021 taaacgttgg ctgagcagtg cgccgagaac gccacccagt aagactgcac tagcgttgat
    10081 aaaagggccg atgaccacaa gagttcctgt taactgtatt gtctgatttt cttattatga
    10141 actttttgcc ttgttgatgg cttgtctggt cgcaaagtcc tggcttgcac gctttagcga
    10201 aaggtgccat gattgcgcga attttctcct ctctgtacgg agtttgcccg atgcacgcca
    10261 cctccttaca ttctctcgct tatcgccgtt tcgcgcgaaa ctcctcccct tttctgctca
    10321 tgcggtgaag ttaacgcacg ctcactgcag gacaacagta aaatcagagc gtttctgctt
    10381 ttactgatgt ctggcggtcg gagctggtga ccagtttgac ccatatctca tggggcaggg
    10441 ttttccacct tgtccggtat tcttacttcc ccgaaacggg tttgcgctta tgaaatcaat
    10501 gaatattgcc gccagtagtg aactggtatc ccgactttct actcatcgcc gcgtggtggc
    10561 gttgggagat actgatttta cggacgtcgc ggcagtcgtc attaccgctg cggatagccg
    10621 cagtggcatt cttgcgttgc ttaagcgcac cggttttcat ctaccggtgt ttttgtattc
    10681 cgaacatgtt gttgaattac ctgcgggcgt tacggcggta atcaacggca acgagcagca
    10741 gtggctggag ctggaatccg cagcctgtca gtatgaagag aatttgctgc caccgtttta
    10801 tgacacgctg acgcagtacg ttgagatggg caacagcact tttgcttgcc ctgggcatca
    10861 acatggtgcg ttctttaaaa agcatcctgc cggacgccat ttttacgatt tctttggtga
    10921 gaacgtcttt cgcgccgata tgtgtaacgc tgacgtaaaa ttgggcgatc tgcttattca
    10981 tgaaggatcg gcgaaagatg cgcagaaatt cgcagccaaa gtctttcatg ccgataaaac
    11041 ctattttgtg ctgaacggca catcggcagc gaataaagta gtgacgaatg cgctgttaac
    11101 gcgtggcgat ctggtgctct tcgaccgtaa caaccataag tcgaatcatc acggcgcgct
    11161 gattcaggcg ggggcgacgc cggtctatct ggaagcttca cgtaacccgt ttggtttcat
    11221 tggcggtatt gatgcgcact gttttaatga agagtatctg cgccagcaaa ttcgcgacgt
    11281 tgcgccagaa aaagcggagt tgccgcgccc gtttcgcctg gcgattattc agctgggaac
    11341 ctatgacggc actgtctata acgcccgtca ggtggtcgat accgttgggc atctgtgtga
    11401 ttacattctg tttgattccg cgtgggtcgg ttacgaacag tttatcccga tgatggcgga
    11461 tagctcgccg ctgctgttag aacttaacga aaacgatccg gggatctttg tgactcagtc
    11521 ggtgcacaaa cagcaggcgg gattctcaca gacgtcgcag atccataaaa aagataacca
    11581 tattcgcgga caggcgcgtt tttgcccgca taagcggttg aataatgcct ttatgctcca
    11641 tgcttctacc agcccgttct atccgctgtt tgctgcactg gacgttaacg ccaaaattca
    11701 tgaaggggag agtgggcgtc ggctgtgggc ggagtgcgtt gagataggga ttgaagcgcg
    11761 caaggcgatt cttgcgcgct gtaagctgtt ccgcccgttt atcccgcccg ttgttgatgg
    11821 caaattgtgg caggattatc cgacatcagt gttagccagc gaccgccgtt ttttcagttt
    11881 tgagccgggg gcgaagtggc acggctttga aggatatgcc gcggatcagt attttgttga
    11941 tccgtgcaag ctgttactca ctacacctgg tatcaatgcc gaaactggcg aatatagcga
    12001 ctttggcgtt ccggcgacga ttctggcgca ctatctgcgt gagaacggca ttgtgccgga
    12061 gaagtgcgat ctcaattcca ttctgttctt attaacgccg gcggaaagcc acgagaagct
    12121 ggcgcaactg gtggcgatgc tggcgcagtt tgaacagcat attgaggatg actcaccgct
    12181 ggctgaggtg ttgccgagcg tttataacaa atatccggtg cgctatcgcg actacaccct
    12241 gcgccagttg tgtcaggaga tgcacgatct gtatgtcagt ttcgacgtca aagacctaca
    12301 aaaagcgatg ttccgccagc agagtttccc gtcagtggtg atgaaccccc aggatgcaca
    12361 tagtgcttat attcgcggtg aagtggagtt ggtgcggatt cgtgatgccg aagggcgaat
    12421 tgcggcagaa ggggcgttgc cttatccacc aggcgtgctt tgcgtggtac ccggagaagt
    12481 ctggggtggg gcggttcagc gttatttcct tgcgctggaa gaaggggtga atttattgcc
    12541 gggattttcg ccggagctgc aaggtgtcta tagcgaaacc gatgcggatg gcatgaaacg
    12601 attgtacggt tatgtgttga agtaagaaaa aaacgggtca ccttctggcg acccgttttt
    12661 ctttgcgtaa ttagtggctc accgtctgtg tgcctgtcgg gacacgaacg tgtttatatt
    12721 tgaacatcgc catgaacgcg aaggccagaa ccacggagta acctgcgaaa atcagccata
    12781 cggtctgcca gtcggtaatg ccgttttggg tgtacatctc aaccacttta ccgctcacga
    12841 tgccgccgag gatacagccg aagccgttgg tcatcatcag gaacatcccc tgcgcgctgg
    12901 cgcgaattgc cgggctaact tctttttcga caaacaccga accagagatg ttgaagaagt
    12961 cgaatgcgca accgtaaacg atcatcgaca gtaccagcag tacagtaccg aacggagtcg
    13021 ggtcgccgta agcaaacaga gcaaaacgca ggatccacgc cacaatactg atcatcatta
    13081 cgttcttaat accgtagcgg cttaagaaga acgggatggt cagaatgaac agggtttcag
    13141 agatctgcga aatcgacatg atgattgacg catgctgcac gatgaagctg ctggcaaaca
    13201 tcggatcttt gtcgaagctg tgcaggaagg tattaccgaa catgttggta atctgcagtt
    13261 ccgcgcccag cagcattgag aagatgaaga agattgccat acgcttgttt ttaaacagag
    13321 cgaatgcatc gaggcccagc agggttgtcc agctctgatt cgcttgctgt ttagcaaccg
    13381 gaatatgcgg cagagtcagg gtaaacagaa ccagaatggc ggaaagcgct gcgccaatat
    13441 acagctgcat gtggcttaat tcgaagccgg acaggctcac cacccacatt gccatgataa
    13501 agccgatggt gccccagata cggattggcg ggaagtcagt aacgatatcc atcccggcat
    13561 tttgcaggcg atagtaagag atggtgttga ttaacccaag cgttggcata taagcaaacg
    13621 agttaatcaa tatcacaagg aacatcgcct ccggcgtagt gacctctgcc gccatgaaca
    13681 gcgtgatagc gccaatggtg tggcaaatgg catagaccca tttcgcactt aaccatttgt
    13741 cggccacaat ccccagcagc gcaggcataa agaccgcggc gatacccagt gaactataaa
    13801 ctgcgccaat agaagcaccg tcaaacttca gggtaacaaa catataggag ccgagggtcg
    13861 tcagccaact tccccacaga cagaactgca gaaaagagag gattttcagc tgcagcttaa
    13921 gattcatgtt aatttcctca catcgtgatg cggatgagtg tttgaaaaag cacatttgac
    13981 cgtgcgagat gatgggattc tatcaacttc gcggactggt tttttgttac ctgtggcaaa
    14041 taattgcaaa cattcccaat ttgcaaaacg aaattgtgat gtggataaca tttttacccc
    14101 tgagcatcgt caggggcggt tagtggaatt atcggcggcg gtaggatttt tgcgcggtat
    14161 tcactttata aagataacgg cgagattccg cagagggatg gcgggtcgtc agcgtttgat
    14221 aaacatcgcc cggcgtcatg gtgttaataa tattggccgc ctgaatctta tcattcgaaa
    14281 agactcgcag cacgctgcct gcgccgccgt tataggcggt gatgacggca taacgccggg
    14341 atgtagggtt atcaattccg ccgagataaa cgttgttcag catcgccaga tacgcggtgc
    14401 cggtatcaat attgctggca ggatcaaaca agaaactgcg gctcggcgtg ccggatttcc
    14461 cctgcgaacg gaacacatct ttcccggcag tatgttgtac cacctgcatt aaccccagcg
    14521 catcggaacg gctgaccgca tacgggttaa aggaagattc agtctgcata attgccagaa
    14581 tcagcgactc atcaacgcca tatttccgtg acgcctggcg gaccatgccg agatatttgt
    14641 gcgcacgttt atcaaggtgg ttcggcacca tgttaatggt gacgctgtag atgatacgca
    14701 gcccgttgct gcggctcttc agacggtttt tcagcagata atccgcgaag ttgcttgcgc
    14761 gaccttccca gcgaatcggc tgcccggtgt tgtccaccac ctgaccgtaa aggaaaggtt
    14821 ctttcgaaat cgtaatatca tcaacgtcgg aatagagatc gaccgaactc ggatcgtcgc
    14881 ccatcagcaa cgttttgata attgcccggc gcaggtgcgc ggcaggttct gtcccggcga
    14941 tggtttcgat agtaatcgta ccgtcatcga agttgatgtg gctgcgggtc tgatattgat
    15001 cggtgtattt cacgtagtcc ttaggaccgg cgatcactac ctctttgaat ccccagatgt
    15061 tctcaatatt gtgggcaaat tgccccatca gaatatcaaa accgttggta tctttgaccc
    15121 aggcttcgtt ataggtatcg ccttttttgg tcgtcgaaca ggagatgagc aacggcgcaa
    15181 tcaaagccag cgcgagatat tttttcatca ttccgggtgc gtgttgtgtt tgtttttatg
    15241 caagttgccg gaggcgtgct ccggcactgt ttttattttt tatcttccgg cgtatagccc
    15301 tcgatatgca cctctttacc ctcgaacagg aagttgacca tctcctgctc cagcagcttg
    15361 cggtgctcgg cattcatcat gttgagtttc ttttcattaa tcagcatggt ttgtttgtgc
    15421 tgccactgcg cccaggcttc tttggagatc tcgttataga tgcgttttcc cagttcgccg
    15481 gggtacagct gaaaatcctg accttctgct tcacgttgca ggaaagtaca aaaaatcgtt
    15541 ctgctcataa atcatcctct ttatcgactc acgcgctaaa ccggcgcgcc agtgcgtaac
    15601 tgctgtaaca aacgctccac gggagccgcc aggccaactg acggcggttg cgctaagtta
    15661 taccagagcg cattgtcttc atccatgcag ccggtgaatg acgacacggg aagccacata
    15721 ggcacaatat ctaagtggaa atggctgaag gtatgccgaa acgcggtcag ttgcgtcagg
    15781 ttatcggcag caatctgccg ttgcgccagc catagtcgca aactttcttc atcggcaaac
    15841 tgcgggaaac agtataaacc gccccacaat ccactcggcg ggcgctgcgc cagcaatacc
    15901 tcatcatcat gctgtaacag taaaaagtaa cccgtgcgct ccggtagcgt ctgctttggt
    15961 tttttgcccg gataaagcga ccagctattg ttggcggtgg caatacaccc gttttgtagc
    16021 ggacagagcg agcatttcgg cttcgagcgc gtgcaaatca tcgcgcccaa atccatcatc
    16081 gcctgattaa accgctccac gccgagcgcg ggcgtcacct gctcgctcaa actccacagt
    16141 ttattctcga cctctttttt cccaggccag ccgcttacag catagcagcg cgccagcacc
    16201 cgtttgacgt taccgtcgag aatcggaaag tgcttaccca gagaaagcga gagaatcgcg
    16261 cctgcggtgg aacgcccgac gcccggtaac gccgcgactt cttcaaaggt ttccgggaat
    16321 ttaccgccgt gtaaggtcgc cacttgttgt gccgctttat gcagattgcg cgcgcgggcg
    16381 taatagccaa gcccggtcca caagtggaga acttcgtcga gcggcgcatt ggcgagatcg
    16441 gtcaccgttg ggaagcgcgc cataaagcgt tcaaaatagg ggataacggt cgcaacctga
    16501 gtttgttgca acatcacttc tgagagccat actttgtagg gcgtcttgtc aatttgccag
    16561 ggcagcgttt tccgcccgta tttatcgtac cagtccagaa cctgggctga aaattgcgac
    16621 gcttgcatgg tcaccgaatt cactgttatt gggggcaaga ttgcagcaca gcgacggcag
    16681 ggtgtaaacc ggaactttcc gcagccacgg ccttatcatg cttgcatccg gcaactaact
    16741 ttggataatg cccgttttca gaacactttc acaagcgact aaatctttat gaaaaacgac
    16801 gtcatctcac cggaatttga tgaaaacggc cgcccgctgc gccgtatccg tagttttgtg
    16861 cgccgccagg ggcgactgac caaaggccag gaacatgcgc tggaaaacta ctggccggtg
    16921 atgggcgttg agttcagcga agatatgctg gatttccctg cgctttttgg ccgtgaagcg
    16981 ccggtgacgc ttgagattgg tttcggcatg ggggcgtcgc tggtggcaat ggctaaagat
    17041 cgccccgagc aggacttcct cggcattgaa gtgcattcac cgggcgttgg tgcgtgcctg
    17101 gcttctgcgc atgaagaggg cttaagcaac ctgcgcgtga tgtgtcacga tgcggttgaa
    17161 gtgctgcata aaatgattcc tgacaattca ttgcgcatgg tgcagctctt tttccctgac
    17221 ccgtggcaca aagcgcgcca taataaacgc cgtatcgttc aggtgccgtt tgccgaactg
    17281 gtaaaaagca aactgcaact ggggggcgta ttccatatgg cgaccgactg ggaaccttat
    17341 gcggaacata tgcttgaagt gatgtcttct attgacggtt ataaaaacct gtcagagagc
    17401 aatgattacg taccgcgtcc ggcatcacgt ccggtgacga aatttgaaca acgtggtcat
    17461 cgtcttggtc acggagtatg ggacttaatg ttcgagaggg tgaaataatg gcaaagaacc
    17521 gtagccgtcg tctgcgtaaa aaaatgcaca tcgacgaatt ccaggaatta ggattttcgg
    17581 tggcatggcg attcccggaa ggtacatcgg aagaacagat tgataaaacc gttgatgact
    17641 ttattaacga ggttatcgaa ccgaacaaac tggcctttga cggcagcggt tatctggcct
    17701 gggaaggtct gatctgcatg caggaaatcg gcaaatgcac cgaagaacat caggcgattg
    17761 tgcgtaagtg gctggaagag cgcaaactgg gcgaggtacg caccagcgaa cttttcgacg
    17821 tttggtggga ctaagaaagc atacgggcga tgacaaatgc aaaactgcct gatgtgctac
    17881 gcttatcagg cctataaaga tgcacgatcg agtaggctgg ataaggcgtt tacgccgcat
    17941 ccggcatgga aaacgcgcac tttgttatca atctggggcc agcaaatgct ggcctgattt
    18001 gttcttgagg gaagactatg atgcgcaaaa tgctgctggc ggcagcactt tcagtgacgg
    18061 caatgaccgc tcacgccgac taccagtgca gcgtcacgcc gcgtgacgat gtgattgtca
    18121 gcccgcaaac cgtgcaggtg aagggcgaaa acggcaatct ggtgatcacg ccagacggca
    18181 acgtgatgta taacggtaag caatattccc tgaatgccgc ccagcgcgag caggcgaagg
    18241 attatcaggc tgaactccgt agcaccctgc cgtggattga tgaaggcgcg aaaagccgcg
    18301 tcgaaaaagc ccgtattgcg ctggataaaa ttatcgttca ggagatgggc gaaagcagca
    18361 aaatgcgcag ccgtctgacc aaacttgatg cgcagctgaa agagcagatg aaccgcatta
    18421 tcgaaacgcg cagcgatggc ctgacgtttc actataaagc cattgatcag gttcgcgccg
    18481 aaggccagca attagtgaat caggcaatgg gtggaatttt acaggacagc attaatgaaa
    18541 tgggcgcgaa agcggtgctg aaaagcggcg gtaacccatt acagaatgtg ctgggaagcc
    18601 tgggcggtct gcaatcctca atccaaaccg agtggaaaaa gcaggaaaaa gatttccagc
    18661 agtttggcaa agatgtttgt agccgcgttg tgactctgga agatagccgc aaagccctgg
    18721 tcgggaattt aaaataatcc tctattttaa gacggcataa tactttttta tgccgtttaa
    18781 ttcttcgttt tgttacctgc ctctaacttt gtagatctcc aaaatatatt cacgttgtaa
    18841 attgtttaac gtcaaatttc ccatacaaag ctaagggata atgcgtagcg ttcacgtaac
    18901 tggaggaatg aaatggagtt tttcaaaaag acggcacttg ccgcactggt tatgggtttt
    18961 agtggtgcag cattggcatt acccaatatc accattttag caactggcgg gaccattgcc
    19021 ggtggtggtg actccgcaac caaatctaac tacacagcgg gtaaagttgg cgtagaaaat
    19081 ctggttaatg cggtgccgca actgaaggac attgcgaacg ttaaaggcga gcaggtagtg
    19141 aatattggct cccaggacat gaacgatgat gtctggctga cactggcgaa aaaaattaac
    19201 accgactgcg ataaaaccga cggcttcgtc attacccacg gtaccgacac gatggaagaa
    19261 accgcttact tcctcgacct gacggtgaaa tgcgacaaac cggtggtgat ggtcggcgca
    19321 atgcgcccgt ccacgtccat gagcgcagac ggtccattca acctgtataa cgcggtagtg
    19381 accgcagctg ataaagcctc cgctaatcgt ggcgtgctgg tggtgatgaa cgacaccgta
    19441 ctggacggtc gcgatgtcac caaaaccaac accaccgacg tagcgacctt caagtctgtt
    19501 aactacggtc ctctgggata cattcacaac ggtaagattg actaccaacg taccccggca
    19561 cgtaagcaca ccagcgacac gccgttcgat gtctctaagc tgaatgaact gccgaaagtc
    19621 ggcattgttt ataactacgc taacgcatcc gatcttccgg ctaaagcact ggtagatgcg
    19681 ggctatgatg gcatcgtcag cgctggtgtg ggtaacggta acctgtataa atccgtgttc
    19741 gacactctgg cgaccgccgc gaaaaacggc actgcagtcg tgcgttcttc ccgcgtaccg
    19801 acgggcgcta ccactcagga tgccgaagtg gatgatgcga aatatggctt cgtggcctct
    19861 ggcacgctga acccgcaaaa agcgcgcgtt ctgctgcaac tggctctgac gcaaaccaaa
    19921 gatccgcagc agatccagca gatcttcaat cagtactaat cacctcgccc cggtatcgtg
    19981 ccggggcttt ttcacttcag actcacgtcc attgccaatt ttcatcaccc taatgataat
    20041 caccggaata aattattccg cgcgagggtt ttcgggtgaa aaagcaatgg attgttggta
    20101 cggcgctgct tatgttgatg actggtaatg tccgggcaga tggtgaaccg ccaactgaaa
    20161 atatcttaaa agatcaattc aaaaagcagt atcacggcat tctcaagctt gatgccatca
    20221 gcttaaaaaa tcttgatgct aagggtaatc aggccacctg gtcagcggaa ggcgatgtct
    20281 cttccagtga cgatctctat acctgggtcg gtcagttggc agattacgaa ctgctcgaac
    20341 agacctggac gaaagataaa ccggtaaaat tctcggcgat gttaaccagt aaaggaacgc
    20401 ccgcttctgg ctggtcggtg aacttttact cttttcaggc ggcagccagc gatcgtgggc
    20461 gggtggtcga cgatatcaaa acgaataata aatatctgat cgtgaatagc gaagatttca
    20521 attatcgctt tagtcagctt gagtctgcgt tgaataacca gaagaactcg attcctgcgc
    20581 tggaaaagga agtgaaagcg cttgataagc aaatggtggc ggcgcagaaa gcagcggatg
    20641 catattgggg aaaagatgca aacggtaagc aaatgacccg cgaagacgcc tttaaaaaaa
    20701 ttcaccaaca gcgtgatgag tttaataaac agaatgatag cgaggcgttc gcggttaaat
    20761 atgacaaaga ggtttatcaa ccggcgattg cggcatgtca taaacagagt gaagagtgtt
    20821 atgaagtgcc gattcagcag aagcgagatt tcgatatcaa cgaacaacgg cgacagactt
    20881 ttctgcaatc acaaaaactt agccgtaaat tgcaggatga ctggataacg ctcgaaaaag
    20941 ggcaatatcc gttaacgatg aaagtttcag aaataaacag taaaaaagtc gcgattttga
    21001 tgaaaattga cgatatcaat caggctaatg aacgctggaa aaaagacacc gaacaactgc
    21061 ggcgcaatgg ggtgattaag taactcatgt taagtgcctt tgccggataa tataaaaagt
    21121 tatccggcaa attatttttt acattagccc tagtagctgt ggaatgaaca aggatatttg
    21181 cgggatgtaa gtgatcatca tcaacgcgat taacaatgcg gcataaaatg gcagcaatgg
    21241 tttgatcaaa tgttgaatct tcacacctga aatagaacaa ccaacaaata gcgcactacc
    21301 caccggcggt gttaacagcc caatacaaag gttagccacc atcataatac cgaaatgtac
    21361 cggatccatt cccagctctt gtgcaatcgg caagaagatt ggtgtgaaga ttagtacagc
    21421 cggtgtcatg tccataaaga tgccgacaat taataagacg atattaatca gcagcaatat
    21481 gatcagtggg ttatcagaaa tccccatcaa tgcgtcgctg atcatataag gaatatcagc
    21541 attggtcatt gcccaggaca tgcccacgga aaaaccgatc aacagtaata caatcgaagt
    21601 ggtaacaacc gattccagaa tcagtttggg taaatcgcgc cattttactt cccggtaaat
    21661 cagcaccgag agaataaatg tgtagacaac ggcaattgct gacgcttcag ttgcggtaaa
    21721 aatgccgccc agaatgccgc ccattacaat gaacaccaga agcaaacttg gtaaggcatc
    21781 cagaaaagct ttacatgcct gagcaaaggt tgctctttct gaaagtgggt agccacggcg
    21841 tttagcaata atgccgcaga ctatcattac cgcgagaccc attaagatcc ctggcaaata
    21901 tcccgccata aataacgatg ctacagaaac gccgccagct gtcagggaaa acacaattaa
    21961 cacgtttgag ggcggtatca gtaatccagt aatacaagat gagacgttta cagcggtaga
    22021 aaatgccgga tcataacctt ctttggtctg aatgggattc agtgtgccac caacggcagc
    22081 agctgccgca acggctgaac cagaaatgga accaaacatc atattcgcca ggacgttcac
    22141 atgaccaagc gaaccaggaa cgcggccaac cattacctgg gcgaggttaa tcaaacgtat
    22201 cgcaataccg ccgctgttca tcagtgttcc ggcaaagatg aaaaagggga ttgccaacag
    22261 ggcaaagtta tccagtccat tagccaatcg ttgcgaaaca gtgattgccg cgatatccca
    22321 gggaaacatc aacagcattg aagctacagt cgcaatagcg attgagaacg aaatgggcac
    22381 accaataaag accagcacaa aaaaacagcc aaacagtgta atcgcgatat aactatccat
    22441 taactggcct cctgcgcagg ttgtttcagt tgttgaatgc tttgattaat aaaacacaag
    22501 gcataaaaca tcatcaccac tccacttaat ggcagaatga tatatacgta tcccatcggt
    22561 atttgcattg cggcagatac ctgtgatgtt gccagtgttt tatcaattaa tttcaggcca
    22621 ccagtcacaa tgaccgaacc ggcaaaacct aaaataagaa cgttaatgac tatactaaga
    22681 agtaattgct tgcgcttatt cagggcgaga gcaaataaat cgatagagag atgtctctgc
    22741 gcgccgacgg tatatgcagc acctaataat cctacccaga tcatcaaaaa gcgtgccagc
    22801 tcatcggtta atgtacttgg ctggttaagt acgtagcggc taaatacttg ccagactacg
    22861 catacaacca gagcaagcat tacggcgaca gaaaacgcgg caatgacgcg gtctaccgca
    22921 agtttcaggc ggttaaatat cattgtaagc atcctggaaa atcagggcca gagactggcc
    22981 cgaaatatta ttgagcagcg ttgtcgaatt tctctaacaa agcagcttgt tttggatctt
    23041 ttttgaagtc gtcaaacaga ggttgaactg cggcacggaa tggggcttta tcaaccttaa
    23101 cgatttcacc acccatagct ttcgcctgag cacgtgtgtc agcgtcaatt ttttcccaca
    23161 atttttgttg ataagcttcg gatttcttcg ccgctgtttc gaggatttgt tgctgttctg
    23221 gggttaattt attccatgtt ttggtggaga taaccaaaaa gtcaggaatt gaggcatgtt
    23281 catcttcaga aaagactttg gcaatttcga tatgtcgagt ctgcacccag gaaggcacgt
    23341 tattttctgc gccatctact acaccttgtt gcatcgcagt ataaacttcg ccaaatgaaa
    23401 ttggagtggg tgagccaccc atcagttcaa tcattttgat ggtggtcggg cttggctgaa
    23461 cacgaatttt cagcccttta agatcttctg gcttagtaat cggttttttc gcatagaaac
    23521 tgcgagtacc cgctacataa gctgataaag caaaaaagcc tttatctttt gttgaatcca
    23581 taatttcttt accaacttca ccaaaaacca ctttgttgaa gtgcgcctga tctttaaata
    23641 agaacggcaa attatagata gcataaatat tatcaaaaga ctccagatca ctggcggaac
    23701 ctttggtcat atccagtgca ccattttgca acaactccat agtttctcgc gcattaccca
    23761 tctggctgct ggggtaaata cggatcacca tattaccttt tgacagctgt tttacttctt
    23821 tcgccagctc ttcaaaactc tgatgtacaa catgacttct ttccaggttg tgtgctaact
    23881 ttaacgtcac tttttctgcg gcgtttacgc cagtgctaac cagagccagc aaggatgcgc
    23941 agactaatga gcgggtaaat gacctcattt tcataaaaaa actccctgtt gaagaatgga
    24001 aattggttga tatctaattg tgcttatcag tgctttgtgc gacaaattgg ttaacctgtc
    24061 tgacaacttg tctatgttta tcacaacttg aaataaagtc agaaattaaa tattaatttt
    24121 gtgcgccagg ttgtac
//
LOCUS       DAPHCV010000080        22894 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.080, whole genome shotgun sequence.
ACCESSION   DAPHCV010000080 DAPHCV010000000
VERSION     DAPHCV010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.080"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1259)
                     /locus_tag="RH033_003149"
     CDS             complement(<1..1259)
                     /locus_tag="RH033_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc22 family transposase"
                     /protein_id="HDV2992300.1"
                     /translation="MSSSLPDDINALKRLLAEQEALNRALQEKLNEREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLKALQKESDTLTGRVDDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAASCCPECGGSLSYLGEDAAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLISKYAEHTPLYRQSEMYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALQDYVLTDGKLHADDTPVPVLLPGNKKTKTGRLWTYVRDDRNA
                     GSTLAPAVWFAYSPDRKGIHPQTHLAGFSGVLQADAYAGFNELYRDGRITEAACWAHA
                     RRKIHDVHVRTPSALTEEALKRIGELYAIEAEIRGMTAEQRLAERQLKTKPLLKSLES
                     WLREKMKTLSRHSELAKAFAYALNQWPA"
     gene            complement(1308..1655)
                     /gene="tnpB"
                     /locus_tag="RH033_003150"
     CDS             complement(1308..1655)
                     /gene="tnpB"
                     /locus_tag="RH033_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004966400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDV2992301.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTTLKDDPMSGHVF
                     IFRGRNGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTPAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(1652..2056)
                     /locus_tag="RH033_003151"
     CDS             complement(1652..2056)
                     /locus_tag="RH033_003151"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDV2992302.1"
                     /translation="MKSLTAVRKKSPNYPVEFKIKMVELSHRPEISVAQLAREHGIND
                     NLLFKWRQYWREGKLRPPSTTENNVPELLPITLDAEDVVPTTSPRSQPVATATPESLN
                     ISCEVTFRHGSLRLNGAISENILNLLIRELKR"
     gene            complement(2136..9674)
                     /locus_tag="RH033_003152"
                     /pseudo
     CDS             complement(2136..9674)
                     /locus_tag="RH033_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554175.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
     gene            complement(9687..11459)
                     /locus_tag="RH033_003153"
     CDS             complement(9687..11459)
                     /locus_tag="RH033_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HDV2992303.1"
                     /translation="MQHRQKNILTKTSLLSRALSVPCCDMFRRGSPWICYLSLSVFSG
                     CFIPAFSSPAAMLSPGDRSAIQQQQQQLLDENQRQRDALERSAPLTITPSPETSAGTE
                     GPCFTVSSIVVSGATRLTSAETDRLVAPWVNQCLNITGLTAVTDAVTDGYIRRGYITS
                     RAFLTEQDLSGGVLHITVMEGRLQQIRAEGADLPARTLKMVFPGMEGKVLNLRDIEQG
                     MEQINRLRTEPVQIEISPGDREGWSVVTLTALPEWPVTGSVGIDNSGQKSTGTGQLNG
                     VLSFNNPLGLADNWFVSGGRSSDFSVSHDARNFAAGVSLPYGYTLVDYTYSWSDYLST
                     IDNRGWRWRSTGDLQTHRLGLSHVLFRNGDMKTALTGGLQHRIIHNYLDDVLLQGSSR
                     KLTSFSVGLNHTHKFLGGGGTLNPVFTRGMPWFGAESDHGKRGDLPVNQFRKWSVSAS
                     FQRPVTDRVWWLTSAYAQWSPDRLHGVEQLSLGGESSVRGFKDQYISGNNGGYLRNEL
                     SWSLFSLPYVGTVRAVAALDGGWLHSDSDDPYSSGTLWGAAAGLSTTSGHVSGSFTAG
                     LPLVYPDWLAPDHLTVYWRVAVAF"
     gene            complement(11681..12304)
                     /locus_tag="RH033_003154"
     CDS             complement(11681..12304)
                     /locus_tag="RH033_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDV2992304.1"
                     /translation="MHIRIDYLADKNCFTELNESPRLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWDISQKNVVFNDKRFGCVYNLKA
                     SLSGGPDTFRYHLSHRIRRVVANENTSSPYQLIAREVKAPRQRLKYALETGLQVSALD
                     GLFWFGSQRIAADILRLRKYGMRIATASVDVYDNITGTTRHIPVYYS"
     gene            complement(13059..14090)
                     /locus_tag="RH033_003155"
     CDS             complement(13059..14090)
                     /locus_tag="RH033_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV2992305.1"
                     /translation="MEKTRKRRGTGRVTLQEVANFAGVGTMTVSRALRTPEQVSDKLR
                     EKIEQAVEELGYIPNRTAGALASGHSHTVAVLVPSLTDKASSRFMQSLQQVLNKNEFQ
                     LLLGCHEYNQLKEAEILMTLLQGNPAALVIFGSQLADKTHQILEKTNIPTVNVVGSPF
                     SPAQITIETAFFEASHKLTEHLLEQGYKNIGFIGAHMENRLQRQQLNGWHKAMLSHYQ
                     NTDLVITMPDAASLQLGRYALNEILLRQPELDAVICSHEEIALGIMFECQRRLLKIPG
                     NIAVACLDGSDSCDQTHPTLTSIRIDYKKMGIETGKLLIGLLNNNHDESEESRIVQFN
                     YQIELRQST"
     gene            14361..14804
                     /locus_tag="RH033_003156"
     CDS             14361..14804
                     /locus_tag="RH033_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001497294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDV2992306.1"
                     /translation="MLKNLLNTEVVQVVEQVKDWREAVAISCRPLIENGSIEPRYVDA
                     IYHSHDTIGPYYVVGPGIAMPHARPEEGANKLSLALTLIPSGVNFDADENDPVKLLIV
                     LAATDSTSHIEAISQLAKLFDNEKDIQAILTAKTTQDILSVIARY"
     gene            14820..15107
                     /locus_tag="RH033_003157"
     CDS             14820..15107
                     /locus_tag="RH033_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDV2992307.1"
                     /translation="MKITVVCGNGLGTSLMMEMSIKSILKDLSISADVDHVDLGSAKG
                     TPSDIYVGTKDIAEQLVAQSVAGKIVALENMIDKKAMRERLSVALTELGAL"
     gene            15120..16376
                     /locus_tag="RH033_003158"
     CDS             15120..16376
                     /locus_tag="RH033_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDV2992308.1"
                     /translation="MDFFRFLMSDVLSEPAVLVGLIALIGLIAQKKPVTECIKGTVKT
                     IMGFVILGAGAGLVVSSLGDFANIFQHAFGIQGVVPNNEAIVSVAQKSFGKEMAMIMF
                     FAMVINIMIARFTPWKFIFLTGHHTLFMSMMVAVILATAGMTGVTLIAVGSLVVGVAM
                     VFFPAIAHPYMKKVTGSDDVAIGHFSTLSYVLAGFIGSKFGNKEHSTEDMNVPKSLLF
                     LRDTPVAISFTMSIIFLVTCLFAGADAVKELSGGKNWFMFSIMQSITFAAGVYIILQG
                     VRMVIAEIVPAFKGISDKLVPNARPALDCPVVFPYAPNAVLVGFLSSFAAGLIGMFTL
                     YLLNMIVIIPGVVPHFFVGAAAGVFGNATGGRRGAILGAFAQGLLITFLPVFLLPVLG
                     DIGFANTTFSDADFGALGILLGIIVR"
     gene            complement(16623..16877)
                     /locus_tag="RH033_003159"
     CDS             complement(16623..16877)
                     /locus_tag="RH033_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV2992309.1"
                     /translation="MEQKTLSAEPRRSFSNEFKLQMVTLALQPRGSVARIAREHDIND
                     NLLFKWLRLSLIKGRISRRLPLTNSSGIGVELLPVEMTLG"
     gene            complement(17299..18312)
                     /locus_tag="RH033_003160"
     CDS             complement(17299..18312)
                     /locus_tag="RH033_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDV2992310.1"
                     /translation="MSTRINLWRALFGEKPRILLENSDFTVTSFRYDSGVEGLKIANS
                     RGHLIILPWMGQMIWDAQFDGHGLTMCNMFRQPKPATEVIETYGCFAFHSGLLANGCP
                     SAEDTHLLHGEMACAAMDEAWLELDGDMLRLNGRYEYVMGFGHHYLAQPTVVLHKSST
                     LFDIKMAVTNLASVDMPLQYMCHMNYAYIPNATFSQNIPDEILRLRESVPSHVNPTAQ
                     WLAFNQRIMQGEASLSTLSQPEFYDPEIVFFADKLDAYTDQPEFRMISPDGTTFVTRF
                     YSAELNYVTRWILYNGEQQVAAFALPATCRPEGYLAAQRNGTLIQVAPQQTRTFTVTT
                     GIE"
     gene            complement(18324..19640)
                     /gene="fucP"
                     /locus_tag="RH033_003161"
     CDS             complement(18324..19640)
                     /gene="fucP"
                     /locus_tag="RH033_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HDV2992311.1"
                     /translation="MNDKNIIQMPDGYLNKTPLFQFILLSCLFPLWGCAAALNDILIT
                     QFKSVFSLSNFASALVQSAFYGGYFLIAIPASLVIKKTSYKVAILIGLTLYIGGCTLF
                     FPASHMATYTMFLAAIFAIAIGLSFLETAANTYSSMIGPKAYATLRLNISQTFYPIGA
                     ASGILLGKYLVFSEGESLEKQMSGMNAGQIHNFKVLMLENTLEPYKYMIMILVVVMVL
                     FLLTRFPTCKVAQTSHHKRPSAMDTLRYLARNPRFRRGIVAQFLYVGMQVAVWSFTIR
                     LALELGDINERDASNFMVYSFACFFIGKFIANILMTRFNPEKVLILYSVIGALFLAYV
                     ALAPSFSAVYVAVLVSVLFGPCWATIYAGTLDTVDNEHTEMAGAVIVMAIVGAAVVPA
                     IQGYIADMFHSLQLSFLVSMLCFVYVGVYFWRESKVRTALAEVTAS"
     gene            complement(19668..20588)
                     /gene="rbsK"
                     /locus_tag="RH033_003162"
     CDS             complement(19668..20588)
                     /gene="rbsK"
                     /locus_tag="RH033_003162"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDV2992312.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNLESWGINTTYVEKVPCTSSGVAPIFVNANS
                     SNSILIIKGANKFLSPEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVL
                     LNPAPALRELDMSYACKCDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNII
                     VTMSEKGALWMTRDQEVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAA
                     LFAAFSVTGKGTQSSYPSIEQFNEFLTLNE"
     gene            20894..21676
                     /locus_tag="RH033_003163"
     CDS             20894..21676
                     /locus_tag="RH033_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDV2992313.1"
                     /translation="METKQKERIRRLIEILKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVHKPAPSMPVIQDVPRNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHDHFGVSWFNPEDLATKRKAMARGLRKILLARHALFDEVA
                     SASLAPLSAFDVLISDRPLPADYVTHCRNASVKVITPDSEDE"
     gene            complement(21678..21904)
                     /locus_tag="RH033_003164"
                     /pseudo
     CDS             complement(21678..21904)
                     /locus_tag="RH033_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229544.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT         5357 a         6035 c         5671 g         5831 t
ORIGIN      
        1 gccggccact ggttcagggc gtatgcgaac gctttcgcca gttctgagtg tcgcgacagg
       61 gttttcatct tttcacgcag ccagctttcc agggatttca gcagcggttt cgttttcaac
      121 tgacgttcgg caaggcgctg ctccgccgtc attcccctta tctctgcctc gatggcgtac
      181 agttcgccga tccgtttcag cgcttcctcc gtcagggctg acggggtgcg aacgtgcaca
      241 tcgtggattt tacggcgggc gtgagcccaa caggcggctt ccgttatccg gccatcccgg
      301 tacagctcgt tgaacccggc gtatgcatcc gcctgcagta caccactgaa ccccgcaaga
      361 tgggtctgcg gatggatgcc ttttctgtcc gggctgtaag cgaaccacac cgccggcgcc
      421 agcgttgacc cggcgttacg gtcgtcacga acgtaggtcc ataaccgccc ggtcttcgtt
      481 ttcttattgc ctggcaacag caccgggaca ggcgtgtcat cagcatggag cttaccgtca
      541 gtcagcacat agtcctgaag cgcttcttcc agcggtgaca gtagccggca gcatgcatcc
      601 acccagcccg acagcagtga acgactcagc tccacgccct ggcggccgta catttcagac
      661 tggcggtaca gcggggtgtg ctctgcatac tttgagatca gcacgcgggc cagcagcccc
      721 ggtcctgcga taccccgctc gatgggccgt gaaggcgcgg gggcctgcac gatggcatcg
      781 cactgagtac aggcatgctt ttcacgtaca gtccggataa cccggaaggc gctgcgcatc
      841 agctccagct gttcggcggc atcctcaccc agatagctca gcgagcctcc acattccggg
      901 cagcatgacg ctgccggcag cagccgtttt tcatcgcggg ggagtgattc ggggaacggt
      961 ttgcgggtgc gggtttgacg cagcgggcgc tgcacggccg ggtcgtcaac ccgaccggta
     1021 agggtatcac tttctttctg aagtgccttc aggtcagctt ccatctgtgc gatacgacgg
     1081 gagacttttt ccgagcggct gccgaagttc atccggcgca gcttatccag ctgtgcctgc
     1141 agatggtcta tttcgcgttc acgctcgttc agcttttcct gcagggcacg gttcagcgcc
     1201 tcctgttcgg caaggagacg tttcagtgca ttgatatcgt caggaagtga gctgctcata
     1261 ccgggtatat taccaggctc attcagcgtc gaccaggata aagaggctta caacatagtc
     1321 agggacgtaa gcagtctttt aggctgccgc cagtcgatac cttccagcag catcgccagc
     1381 tgtgccggtg tgaggaacac tttgccatcc cgggctgacg gccaggcgaa gcggccgcgc
     1441 tccagccgtt tggtcagcag acacagtcca tcgccggtag accagaggag ctttacctga
     1501 ctgccattac gcccacggaa gataaaaacg tgacctgaca tcggatcgtc tttcagcgtc
     1561 gtctgcacct ttgccgccag gccgttgaag ccgtttctca tatcggtgat gccagcgacc
     1621 agccagatct ttgtccctga tggtaatggg atcaacgttt gagctcccgt atcagcaggt
     1681 tcaggatatt ttcactgatg gcaccattca gacggagtga tccgtgccgg aacgtcactt
     1741 cacagctgat attgagtgat tcaggtgtcg cagtagctac aggttgtgac cggggggagg
     1801 ttgtagggac aacatcttcg gcatcaagtg ttatcgggag cagctcaggc acgttgtttt
     1861 ctgttgttga aggaggacgt agttttcctt cgcgccagta ctggcgccac ttgaacagca
     1921 aattatcgtt gatcccatgc tcacgagcga gttgcgctac ggagatctct ggtcgatgcg
     1981 agagttcaac cattttgatt ttgaactcaa cgggataatt agggcttttt ttacgcactg
     2041 cggttaatga tttcatggat agcgtccacc atatttggtg tccactattc tctcaggaat
     2101 ttcaggatct gccagacggt gctgagacga cgcttacccc tgtttttacc ggttgcgaca
     2161 acagacagat tattcccggc attcagcgta ctgccggata ctgtgtcact ttcagaatgt
     2221 tgttgtgatt tcgatttctg ggtggtgagc gacaggctga ctcccatcgc attcgggtca
     2281 ccggttgcgg aggccattgt cgcagcctgt ccggcctgca caccagacag cgctgtcttt
     2341 gtggcctgca gggctttcag acggctgtca ctgctctcct tcgtctcctg tgcactggta
     2401 accgcattat tgatggcact gcccaccgtg ccggaaaggg caaccgtcag cccgcttttc
     2461 ttctgctcaa atttttcgtc cacagtacga cggtcatgcc ccgggtcaac caccacactg
     2521 tcaccggtaa tgctgatatc ccggttcgca atcacatccg aaccgctgat atgagcctgt
     2581 ttgcccgcgg taatactgac attaccggca gtggagccga tggtgctggc actctgactc
     2641 tgcgttgtcc cggcctcgcg ccggtcgtgc gttgtcttac tgctgccaat ggtgaaacca
     2701 ataccgccgg tacccatcag accggatttc ttcgtttcct taaagcgcca ggacgtatct
     2761 gtactggtgg cagcaagaac atcaacatgg ttacccgccg ccagtgacac atcccggtca
     2821 gccaccacat ccgaaccctc taccgtcagg ttatcaccgg cgttaacggt cacgcggttc
     2881 cccgacagca gggagcctgc ttcacgggtg gcactgtcct cactgatggt gcgggtggtt
     2941 ttcttactga gaaaacctcc gctttttttc ttcgtttcca gatagtgata gtcactttct
     3001 gtcgccgtgg tcagggcaac atcacgaccg gcattcacgc tgatattgcc ggttgcggta
     3061 acggatgacg caacagcggt gatatcccgt cctgcgttga cggtggtgtc accaccgctg
     3121 gcgatttccg ttccctgctg acggactgtc tcgttaatct ctttcttttt cttcgacgta
     3181 tagctgtcgc ctgcgccggc agactctgcc accaggttca catcacgtcc gccccggatg
     3241 accacgttat tttccgcagc cataccggca gcctgactgg caatatcacg accggcaaca
     3301 aggaggaagt tatcgcccgc tgtcaccgtg gacacagctg cgtggctttc atgactttct
     3361 gacctgccgt tgcgactgtt tttgctttcc ctgactgcat tcagactcag gtcgttacct
     3421 gcagaaagca gggcgctgtg cccggcagaa acagaggatg ccgtgacatc cagattatgg
     3481 cctgcagcca tcgccaggtt accgccggcg ctgatgctgc tgccctgtga ggtggtggat
     3541 gatgaactgt tgtcatcagt gtgccagaaa ccggactgac ttttgctccc acttatcagg
     3601 tttgcggcaa tgttgatatc attacccgca gacattccaa ggtctccacc ggacgagacc
     3661 gttgccccgg taatatcaat gtttttccct gcatccagcg aaagtgaatc agtgccttta
     3721 atggtcgcaa ccggaccggt gtccgtaccg ctgagatgca caccaccata ttggctgtca
     3781 ctgcccgcat tccattgctg acgccgggtg atattgctga tgttgccact cacgctttcc
     3841 agttgtacgg ttttaccgct gatgactgag ctgatattgc tgatatcccc gatggcgctc
     3901 aggtccaggc taccgcccgc gcttatcagc cccgcattca ggttgtcgat atagccggaa
     3961 ctgtcgagcg agaggtcgtt ctgtgcgttg atgctgccgc cgctgttggt gatattgccg
     4021 cccgcaagtt gcacgttgtt cccgctgata acgctgccgt tatgcagggt gatatcttcc
     4081 ggcgacagat acagtttcgg gaccatgact gtttgtccgt tgatggtgac tgactcccac
     4141 cacagtatgc tgccgtcaag ctgagcaatc tgttcagctg tcagcgccac accaaactct
     4201 aatcccagtc ctttctgttg tctggccgcg ttatccatca gataccgcat ctgttccgtg
     4261 tctgaaccca gtccgttgag ataacgtgaa cccgtccggc tcagcaccgc gttactgaca
     4321 taccgggtat caaagaccgc atcccccagg aaacgataat ctttttccgg tttcagcccg
     4381 aggcggtcaa gaaaatacga tgagcccaga aactgttttt catcggtata cgacggagcc
     4441 gtttcacgtg gcgcctgacc cggcttcgct ccaagaagct catacagtcc ggcaaacaaa
     4501 tggctgtcca cctgtccgag accatccagt ttcgggttca ccgtaatcag atacggactg
     4561 tccgggtccg tggacggaac caggtatcca ttgttgccgg aaggcagtgg ccagtcatca
     4621 ctgataccgg tctgaccggt cagtggcgaa cctccggcaa tatttttcag ggcacctgcc
     4681 agttcatcgt gccattgcgg agagccaacc accaccggct catactgctg cagcgctgtc
     4741 tgtgtcagac tgtctccgcc ggtctgctga cttaacgtat tcagtacagg tgcagagacc
     4801 accggactga cactacctgc atgtgcagtg gttgttccgt tattgatact gctggtaaaa
     4861 cgggtcttaa catccccgcc cgcctgaata acggaataat acgtcttacc gggcgtgtaa
     4921 tctttttccc ggccatccag tgaaaatctg atggtattgt tttcaaattc cggtgacagc
     4981 aggggcagtt tatccagaga gcctgttgca tagctaccgt aaaacgtttt cgggtcgtag
     5041 cggtatacca gatattcatt ctctgtcccc gtctgccagc tctgattgct taactctctg
     5101 cccgagagtg cgatatcccc attcgccagg ataaatgacg cccggttttc cagtcgttca
     5161 gcctcagcag aaagattacg ccctgacgca atgcggcctg ccggattatc agcaccggtt
     5221 actgttgtga tgttctggct gctgagaaag cgctgtgtgg cactgtcagc aaacggcgcg
     5281 taataataaa gcgtatccat tgtgatattg catgccccgt gcccgttgca gggcgtaccg
     5341 tgctgatttt caacttcacg ggtgaaatag ccatagctgc cgtcaggaag aagggaaagg
     5401 ggaatatcaa ccagagcatt tcccattccc tgaatggatg aggggttagt ccgggttgtt
     5461 gttgtggcag aaaatccctc ccgctggttc agaagatgcc cggttcttac aacaatatcg
     5521 ccctgatgcg tctcaatatt cccggaatta ttgataatct ctgtgtttgc accgccggaa
     5581 gcatccttct gtacccacag actgttgccg gccaggatat caccatgctg gttatgcaga
     5641 cggtctgtaa acagcttcag gttattcccc gcataaatca gcgcactgtt cagcagggta
     5701 ccggccacat tcattgtcag actgcctgcc gtgccggtaa aaccactgat ggtgatatca
     5761 ctccggctgt tcagactcac atcgccaccg ccctgaagtg aacccggtgc gttaaggaaa
     5821 agacgctgtg cgctgaaaac actgttgcct ttaccggcag tcagcgttcc attgttggtg
     5881 aatgcctctc cggcaccgag caccatggca tcaccctgca tgacaccgcc gttggtgatg
     5941 gcattttgcg acgtgacgga aagggttttc cctgcggcca gggtaccatg attcgtgagg
     6001 gcagcaatca gtttcagtgt gacatcaccg gtggccacca cctgcccctg accactgaag
     6061 tcctgagcgt caagcagcag gttgcctgca ctgtacagcc gccctgtacc attttgcagc
     6121 agtgaactgc ccttgacgcc aagcccggag gttcccagca gggtaccgct gttgctgaat
     6181 gtgtggtaat tcaccagcag gtccgcaccc tgaagcgtac cggtattatt cagcgtggtt
     6241 cctttaacgt cggcactgcc ggtggcaagt acgcgtccgc cgttgacagt attcaccaca
     6301 tccagcagca gggtggcagc ctgtaccagt ccgctgccgg tgttcgccag cacctgcgcc
     6361 gtcagcgtga ggttactgcc ggagaggatt ttgccgtcgt tctgcagacg gtctgtggcg
     6421 ttcagggaaa ccccgcctcc accctgtatc gtgctctggt tactcagggt cgcagtactg
     6481 acattcagtg cattccggct catcagaaca ccaccggaac ggttgttcac gccaccggag
     6541 gctgtcagcg tcagcgtttc gccctgcaga tgcccgccgt ttgtgagttg tcctgccgtg
     6601 atggtggtga catttccctg taattgcccg tcgtttgtga cactgtctgc cttcagcgtc
     6661 agcacacctg cactgagcag ttttccgttc gcctgattgt tcagcgtctg attcaccgtg
     6721 agcgtgagag tatccacacc ggtgatgtca cccgcactgg tcagtgagtt cgccttcagg
     6781 gtcagatttt ttgcaatcca ttgtccgctg ttgcttaaat tcagtgcact gagcgccatt
     6841 tcaccgttcg aggtgacttt gctgcccgct gtgctgacga gctcacccgt cagacgtgca
     6901 gtcaggctgt cagccgcctg gatcgccccg ctgtttgcca gactgtctgc ggtgatcagc
     6961 acccgtttgc cctgccagtg tccggaactg gtaatactgc ctgcggtgat tgtcagatcg
     7021 ccgctggtca gcaatgaacc tccgttattc atcagcgcag gttgagggga tgccatacgg
     7081 gcggcaagcg tcagtgcggc gatcccggtg agcgtgccac tgttggtgac actgttctgg
     7141 cgaatcgtga catggttacc ctggacagtg ccgcggttat ccagtgaatt tccatcaagg
     7201 gagagcgtgc cggccgaaag cagactgccc cggttgtccg tggtggctgc tttcagcgtg
     7261 gtgtcaccct ggctcatgat atcgccggta ctggtcaact gaccggttgc cgaagcagta
     7321 aggttaccgg ttgccagcac ggaaccactg ttcgcccatc tgtccgcctg cacggtgata
     7381 ttctgtccct gcgtggtccc tgcggtattc agtgttttgc cccggaggga gaggtcgtcc
     7441 gccgtcagcc agcgtccgtg actaccctgt gagagggtgt cgccagcaag cgccagtgca
     7501 ccggcgccct gcaacaggcc gtcaccatcc agcgtggtcg ccctgacgct cagcgtgtca
     7561 gcgatgattt ttcccggatt gctgagggag acagcattta acattaaacc attatcaccg
     7621 gtgataagcc cgctgttgcg gatgtccggt atatccagcg tcaggtctgc agcactgtac
     7681 agcgtgccgt tctgctgatt atcaagcctc tgtgtgttaa cggtgagtga ggcctccccc
     7741 tgcaacagac cgctgttggt cagggtctgt gactgtgtat tcagggcgga accaacaagt
     7801 acgccgctgc tggtcagttc cggcgcactg aggctgagcg acggggcact gcttttcccg
     7861 ctgtgggtga gcttttcact ggcgttcacc accatggtct gttgtgctgc ctgcgtacct
     7921 gcaagacgtg catctctggc gttgatgctg agatttttgc cgctctgaag ttgtgcgccc
     7981 gctgcggtac tcagtttgtc tgcctgaacc cggagggtgt caccggcact ggtttccccg
     8041 tccagcgcca ctgttgtcac attcagcgtc atcgcagcat cgctgtgggt gaccgatttt
     8101 ttaccggagc tcagcgtctg cgcactgacc gtcagccctt tgccgccgga cagcacaccg
     8161 ttctgtgtca catcctgcgc cttcagcacc agtgcatcat cgctcaccag caaacctgta
     8221 ctggtcagtt tcccactggc catgatatcc actttgccct tcgcgccagt gcggccgctc
     8281 tgggtaaagt cgcgggtatt catggtcagg ggaccgccac tgagcaggga gccactgttg
     8341 ctgagcgttg tactgccgag cgtcagggta gctccctgaa cggcgccact gttattcagc
     8401 gtaccggcat tgagtcccgc atgacttttc gccagcaata ttccgtcctg cgtcagcgtg
     8461 gtggcgctgg ccgtgagatt ctgcccggcg gttatctgtc cctgtgttgt cagcgtgtca
     8521 ctggcgacag tcacgatatc gcgggccgcg ttaatctggc tggcggtatc ctgtgtgatg
     8581 tttttcgcgg caagcgttac atcccggccg gcagtcagtt tttcattctg ttgagtgatt
     8641 ctgccgccgg cggtcaggct gaggtccttg tcgctgttaa gcgttccatt gctgagaacg
     8701 atatctctcc ggctgctgac gctcaggtta cctcccgctt tatggtcgcc ggttaaggtg
     8761 acgccctgac cttttgcagt gacggccccc gtggcgagac tgttgttgac agtcagtctg
     8821 ccgctggcat ccagggtgat atcgccatcg cgggcataaa ggttaccaag attaaccccg
     8881 acaccacttt cagtggaggt cagatgaata cgcctggcgt acattccacc gagagcgccg
     8941 gtatcaacgg caactttcgg cacatcacct tcgcccttca gggctctgac acgaccatct
     9001 gcagttacac ggttagcgcc tgcagtgaca gttaaatcct tcgcatgaag cgcggcattc
     9061 acttccgttg cacgggcaat aatggatacg gcatcgctcc gggtgccgtc caggcccgcg
     9121 ccattgatgg tgatactgcc ttcagtcacc tccagcgcct gcaggctgcc gtcggcattc
     9181 atcacaggtt tgcccgtggt gagcgtcgcg tgcggcgtgt tgataaagcc acagccgtca
     9241 caggtgatac catacgggtt ggcaaccatc acattcgccg ctttgccggc cacttccgta
     9301 tagccctgca gcagtgaacg cttaccgccg gtcacttcgt tgatgatacc cttcgcttcc
     9361 ccgcccgctt tcaggttcgg gttattctgt atcagtccac caagctgcgt cggattaagc
     9421 ttaccggtgg cattattgag aatcagccct tccttcccga cgttgtaatc cgtaaaccgg
     9481 ttatgcgaaa tcccggcccc gttcggcgtg gcaatgttca cgaccggcac accatttgcc
     9541 gctttatcca ttccggcccc gttttgtggg gtgatgacgg cccccacagc cggtaacaac
     9601 ggctgcccgg cgataatcgc actgaccagg taactcagca ggcggtaagt gaagcgaacg
     9661 ggaggctgat gcatggtaat aatcccttaa aacgcgacgg caacgcgcca gtaaaccgtg
     9721 agatggtcag gggcaagcca gtccgggtaa accagaggca gtccggcagt gaacgaaccg
     9781 gaaacatggc cactggtggt gctgagcccg gcagcagcac cccacagcgt gccggacgag
     9841 tacgggtcat cgctgtcaga gtgcagccag ccgccgtcca gtgcagccac tgcacggaca
     9901 gttcccacat atggcaggga gaacagagac caggacagct cattccgcag ataaccgccg
     9961 ttattaccgg agatatactg atccttaaag ccacgcactg aactctcacc cccgaggctc
    10021 agttgttcca caccatgaag acggtccggt gaccactggg cataagcgct ggtcagccac
    10081 cacaccctgt ccgtgacggg gcgctgaaaa ctggcactca ccgaccattt ccggaactga
    10141 tttacgggca ggtctcccct tttcccgtgg tcgctttctg cgccgaacca gggcatcccc
    10201 cgtgtgaata ccggattcag tgttccgccc ccccccagaa acttgtgggt gtgattcagc
    10261 ccgacagaaa atgaagtgag tttacggctg ctgccctgaa gcagaacatc atccagataa
    10321 ttgtgaataa tgcggtgctg cagacctccg gtcagtgctg tcttcatgtc cccgttacgg
    10381 aacaggacat gcgacagtcc cagccggtga gtctgcaggt ctcccgtgga acgccaccgc
    10441 cagccccggt tatcaatggt gctgaggtag tcactccatg aatacgtgta atccaccagg
    10501 gtatagccat acggcagact gacaccggcg gcaaaattcc tcgcatcatg tgacaccgaa
    10561 aagtcactgc tccgtccccc gctgacaaac cagttgtcag ccagccccag aggattatta
    10621 aaggaaagga caccatttaa ctgccccgta ccggtactct tctgcccgct gttgtcgatg
    10681 cccacgctcc ctgtgacagg ccattccggc aatgccgtca gtgtcaccac cgaccatccc
    10741 tcacggtcac cgggcgatat ttcaatctgt accggctccg tacgcagacg attaatctgc
    10801 tccatcccct gctcaatatc ccgcaggttc agaaccttcc cctccattcc cgggaaaacc
    10861 atcttcaggg tgcgggcagg aaggtcagcg ccttccgccc ggatttgctg cagcctgcct
    10921 tccatgaccg ttatgtgcag tacgccccct gaaaggtcct gctctgtcag aaaggcccgg
    10981 ctggtgatat atccccggcg tatatagccg tccgtcacgg catccgtgac cgcggtcagc
    11041 cccgtgatat tcagacactg attcacccac ggtgccacca gtctgtcggt ttctgcagac
    11101 gtcagtcggg tggccccact gacaacaatg cttgacaccg taaagcaggg accttcagta
    11161 ccggcagacg tttccggaga cggcgtgatg gtcagcggcg cactgcgctc cagcgcatca
    11221 cgctgacgct ggttttcatc cagcaactgc tgctgttgct gctgaattgc actgcggtca
    11281 cccggagaca gcatggctgc cggagacgaa aatgcgggga tgaaacaacc agaaaaaacg
    11341 gagagggaga gatagcatat ccacggagag ccgcgccgga acatatcaca acaggggaca
    11401 gacaacgcac gggataaaag ggacgttttc gtcagaatgt ttttctgccg gtgctgcatg
    11461 ttctccccgt attctggcct gtgtgatgcc agtgtaaatt catgtcaaaa agtgtcagta
    11521 aagaaattta catcagaaga gaagcatttt tgaacagtaa cccggtaaaa tatcaggaaa
    11581 aatcgaagaa atgtgataaa ggacgtattt ttggctgtga cgagggcaaa accctgttcc
    11641 ggttaccggt catgctccgg cccccacaga ctattttgcg tcaggagtaa tatacaggga
    11701 tatgacgagt tgttccggtt atgttgtcat acacgtccac agaagccgtc gcaatccgca
    11761 tcccgtactt tctcagcctc aggatatctg ccgcaatgcg ctggctgccg aaccagaaca
    11821 ggccgtccag tgcgctcacc tgcaatcctg tctccagcgc atacttcagc cgttgacggg
    11881 gcgctttcac ttcccgggca atcagttggt atggcgatga tgtgttttca ttcgcaacca
    11941 cccgccgaat acggtgagac agatgatacc ggaatgtatc cggcccaccc gaaaggctgg
    12001 ccttcaggtt gtacacgcag ccaaagcgtt tatcgttgaa caccacattt ttctggctga
    12061 tgtcccattc ttcacgcagc gcggcaatca gttgtggtgt acgagtaagc aacaagcgaa
    12121 aaggcagttc aaaactggtg acgtaatcca cattcagcag agcaatacgc agccgttctt
    12181 ctggcccggc ctctgtctgc cggcactcct ccagaacatc ctgccactgc cggcgaaggc
    12241 gtggagattc attcagttct gtaaagcagt ttttatccgc cagatagtca attcgtatat
    12301 gcatactgaa gagcattccg tataaagatt cagctggcaa aactttatca gtctgtaaaa
    12361 actaacggaa gagtcgatat ttctcccgtc catcaccgga tgattgttgc aataccccgt
    12421 ggcatcagag actgaacagc cgtttttaac gcaacgtatt gctctgatgt atcaggccgg
    12481 acaacccgaa aacagccttt caccctgcat tgtccgccag cgcttattac cagccacgtc
    12541 tgttgcagta aattaatcag ttgagaacat tcttcatcaa tcgtgacgct ggctcgcgca
    12601 tggcagaaaa ggtcctgact gagcaacaac aggcatcgta ttgttgctgc tccatgttgt
    12661 ttatatgaac gcatcccgtt tgcaaggctt ccgtgttttt gacgttgacg gtagattgct
    12721 gctctatatc cctattgcag ccggtgctgc gggcctcgat gagattcgct gactctcgcc
    12781 ttattcacgc aacgagcttt aagctcagaa aaacatccaa gtttggtgag tcacagtctt
    12841 acgtgtcgtt gtcatcaata taggaatgga taatctggag agcgacactg cgggctgata
    12901 ttgcggtttt acggcaagtt tttagcatcc gcttctacgc caaatacctg aagtaggaat
    12961 ggatgcgtta gcagcacaca tccggatctg cgcggggaat agccggaaac ggctatccct
    13021 accacaatag ctaactttgc aatagtgccc ttttaggctc atgtactctg acgtaattct
    13081 atctggtagt tgaattgcac gatacgcgac tcttctgatt catcatgatt attattcaat
    13141 agtccaatca gtaacttacc cgtctctatc cccatttttt tgtagtcaat acggatggat
    13201 gtcaaggttg ggtgtgtttg atcgcaactg tctgaaccgt caagacaagc tacagcgata
    13261 tttccaggga tttttaacaa acgccgctga cactcaaaca tgatacccag cgcaatctct
    13321 tcgtggctgc aaatcacagc atccagctct ggttgtcgca gtaatatctc gtttaatgcg
    13381 taacgcccca attgcagact tgcggcatcc ggcattgtga taacaagatc agtgttctgg
    13441 taatggctca gcattgcttt atgccagccg ttcagttgct gacgttgcag gcggttttcc
    13501 atatgcgcac cgataaatcc gatattctta taaccctgct ccagcagatg ttcggtcagt
    13561 ttatgtgatg cttcaaaaaa tgctgtttcg atggtaattt gagccggact gaaaggggaa
    13621 ccgacaacgt taacggtagg tatatttgtt ttctcgagta tctggtgagt tttatcagcc
    13681 aactgagaac caaaaataac taacgccgcc gggttgcctt gaagaagcgt catcagaatt
    13741 tcagcttctt tgagctgatt atactcatga cacccaagca gtagctggaa ttcattttta
    13801 ttaagcacct gctgcaacga ctgcataaag cgggagctgg ctttatctgt cagggaagga
    13861 accagtacgg cgactgtatg actgtgacca gatgcaaggg cgccagcagt tctgttggga
    13921 atataaccca actcttctac ggcctgttct atcttttccc tcagcttgtc agaaacctgt
    13981 tccggtgttc tcagcgcgcg ggagacggtc atggttccga cacctgcaaa gttagcgacc
    14041 tcctgcagcg tcacccgtcc ggtaccacgg cgcttacgtg ttttttccat ttcgactcca
    14101 tggataatta caaccagctg ttttcattac ttttatttta ccattactct ttccagtttt
    14161 tgcccacggt agcaaatatc ctgttgcatt gtatatgcag gaaccgaata tagccaaagc
    14221 atggcggcag agaaatgata gcgctatcac aaagattggt agcgttatca gatagcgcta
    14281 tcaaatgcga caatcatcac attaattaat gcgtaaaata tctactctcc tgttaattat
    14341 catcgggagt tatcagtcgt atgttaaaaa atctattaaa tacagaggtc gttcaggtcg
    14401 tggaacaggt caaagactgg cgggaagccg tggcgatttc atgccgaccg ttgattgaaa
    14461 acggttctat cgaaccgcgt tatgttgatg cgatctatca ctcgcacgac actatcggtc
    14521 cgtactatgt ggttggccct ggtattgcga tgcctcatgc gcgcccggaa gagggggcta
    14581 acaagctgtc attagcactg accctgatcc cttcaggtgt gaattttgat gccgatgaaa
    14641 acgatccagt gaaattactg attgtgctgg ctgcaaccga cagtaccagc cacattgaag
    14701 cgatttctca actcgccaag ttattcgata acgaaaaaga catacaggct attttgacgg
    14761 ccaaaacgac gcaggacatt ttgtctgtta ttgcgcgcta ttaattctga ggaacattta
    14821 tgaaaattac agtggtttgc ggtaacggct taggcaccag cctgatgatg gaaatgagca
    14881 ttaaaagcat tttgaaggat ttgtccatca gcgctgatgt tgatcatgtc gacctgggct
    14941 ccgcgaaagg gacgccgagt gatatctatg tcggcaccaa agatattgcc gagcagttag
    15001 tcgcgcagtc tgtcgcaggc aaaatcgtcg ccctggaaaa tatgattgat aagaaagcca
    15061 tgagagaacg tttgtccgtg gcattaaccg aattaggcgc actgtaagct gggggcgata
    15121 tggatttctt tcgttttttg atgagcgatg tactttccga acccgcggtg ctggtcggtt
    15181 taatcgccct gattggcctg attgcacaga aaaaaccggt aaccgaatgc attaaaggta
    15241 ccgtcaagac cattatgggg tttgtgattt taggcgcagg ggcgggttta gttgtgtcgt
    15301 cactgggtga cttcgcaaac attttccagc atgcctttgg tattcagggt gtcgtgccga
    15361 acaatgaagc cattgtttcc gtagcgcaga aaagcttcgg gaaagaaatg gcgatgatta
    15421 tgttctttgc gatggttatc aacattatga ttgcccgttt cacaccgtgg aaatttatct
    15481 tcctgaccgg tcatcacacg ttgtttatgt cgatgatggt agcggtaatt ctggcaacag
    15541 caggcatgac cggcgttacg cttatagccg ttggctcttt ggttgttggc gtggcaatgg
    15601 tctttttccc ggctattgcg catccgtaca tgaagaaagt gaccggttct gatgacgttg
    15661 ccatcggcca cttctcaaca ttatcttatg tattggccgg tttcatcggc agcaagtttg
    15721 gtaataaaga gcactcgact gaagacatga atgtgccgaa aagtctgctg ttcttgcgcg
    15781 acacgccggt tgctatctct tttaccatga gcataatttt cctggtgacg tgcctgtttg
    15841 cgggtgcaga tgcggtgaaa gagcttagcg gtggtaaaaa ctggttcatg ttctctatca
    15901 tgcaatccat taccttcgca gctggcgtgt acatcattct gcagggtgtg cgcatggtga
    15961 ttgcggagat tgtcccggca ttcaaaggta tttcagacaa gctggttccg aatgccagac
    16021 ctgctctcga ttgcccggtc gtcttccctt acgcacctaa cgctgtactg gttggtttcc
    16081 tgagtagctt tgctgcaggt ttaatcggta tgttcacgtt gtatctgctg aacatgatcg
    16141 tgattattcc tggtgtggtg ccgcacttct tcgtaggtgc agctgcaggg gtattcggta
    16201 atgcaacggg tggacgtcgt ggtgcgattt tgggtgcctt cgctcaaggc ttgcttatta
    16261 ccttcctgcc agtattctta ttgccggtgc tgggtgatat tggttttgct aacaccacct
    16321 tcagtgatgc tgactttggc gcactgggta ttttgttagg gattatcgtt cgctaatatt
    16381 ttgatgacat taatatcctc caggatttca ggaacgtgga ggatattaat tcctcttgtt
    16441 ttctccattt ttctttcttc cagtcaggac tgcatccgat taattgtaag cgctccgaga
    16501 agtacgtagc gtaaggatta ttttacagct gagaagtttc agtgcagcag ttcatgcaca
    16561 cggttcgacg gctagtcatt gatcttcata attacctcgc gtaaaaaacg gggtaaggcc
    16621 gctcatccca gcgtcatctc cacaggtaat aattcaacgc caatgccaga agagttcgtt
    16681 aacggaagac gccgtgatat acgccctttg atcagcgaga gcctgagcca tttgaacaga
    16741 agattatcat tgatatcatg ctcccgggca atacgggcaa cagaacccct tggttgtaaa
    16801 gccagtgtaa ccatttgaag tttaaactca tttgaaaatg acctgcgggg ttcagctgat
    16861 aatgtttttt gttccataac aggtgtccac tagttgaaaa ggtgggtacc tatgttacca
    16921 atactggctt aatggctaca tacagcagtc ggtttacgca tacactttta tagtgcactc
    16981 cggcctcatg aatataacgg tggattaccg ccgaacgaat cggaaaatag atactggaaa
    17041 aaaactgaaa ttgtgaccag tttttgttga ccactacaaa taaataaaag agttgcagtg
    17101 cttgctcggt aagaaaacaa tggaagttga gatcctcaaa gaagctgtgg agtacaccac
    17161 tctacagtag ttgcgcaaat ttggagtgga cgataatgtg atcgtctata agggcaacgc
    17221 tatcatagtc ttgtcctggc gggtaaaaaa gcgcgcttac cttaacgata agcgcgccgc
    17281 tgttcaggcc ttgagtggtt attcaattcc tgtggtgact gtaaaagtgc gcgtttgctg
    17341 cggtgcaacc tgaatcagcg tgccattacg ttgcgcggca agatacccct caggccgaca
    17401 ggttgcaggt aatgcaaagg cggctacctg ttgctctccg ttataaagga tccagcgtgt
    17461 cacataattt agttcagcac tgtagaaacg agtaacaaac gtagtgccat cgggagagat
    17521 catgcgaaac tctggctgat ctgtataagc gtccagtttg tctgcaaaga agacaatttc
    17581 tggatcataa aattccggtt gactcagcgt cgacagagag gcttctccct gcataatccg
    17641 ttgattaaac gccagccact gagcggtggg attaacatgc gaaggcactg attcacgcaa
    17701 tcttaatatt tcgtccggga tattctggct gaatgtagca tttggtatat atgcataatt
    17761 catgtggcac atatattgta gtggcatatc tacagaagcc agattggtta cggccatctt
    17821 aatatcgaac agtgtagagg atttgtgaag gaccactgtt ggttgagcca gataatgatg
    17881 accgaaaccc attacatact cgtaacgccc gttaaggcgt aacatatctc cgtctaattc
    17941 cagccatgct tcatccatcg cggcacaggc catttcaccg tgtagcagat gagtatcttc
    18001 cgcagatggg cagccattag ccagcaaacc tgaatgaaaa gcaaaacagc cataggtctc
    18061 tatcacctct gtcgccggtt taggctggcg aaacatattg cacatggtga ggccgtgtcc
    18121 atcaaattgc gcatcccaaa tcatctgccc catccaggga agaataatca aatgtccacg
    18181 actgtttgca attttaagcc cctcgacacc gctgtcatag cgaaaagacg tgacagtaaa
    18241 atcactattt tccagcaaga tacgaggttt ctcgccaaaa agcgcccgcc acaaattaat
    18301 acgcgtactc ataacggttc tcctcaggac gctgtgactt cagccagtgc ggtacgtact
    18361 ttgctttcac gccagaagta gactccgaca tagacaaagc agagcataga aaccaggaat
    18421 gaaagctgta gtgagtggaa catatctgca atatatccct gaattgctgg aaccaccgcg
    18481 gcaccgacaa tagccataac aatgactgct cctgccattt ctgtatgttc gttatcaact
    18541 gtatccagtg ttcctgcata gatcgtcgcc cagcaagggc caaacaaaac acttaccagg
    18601 acggcgacat agaccgcgct gaaacttgga gccagtgcaa catatgccag gaacagcgcc
    18661 cctataacgg aatagagaat caatactttt tccggattaa aacgcgtcat aaggatgttg
    18721 gctataaact tgccaataaa gaagcaggca aagctataga ccatgaagtt tgaagcatca
    18781 cgttcgttga tatcgcccaa ctccagcgcc agacgaatgg taaatgacca tactgcgacc
    18841 tgcataccca cataaaggaa ctgcgccaca ataccgcgac gaaagcgcgg atttctagcc
    18901 agatagcgca gcgtatccat tgctgacggg cgtttatggt gacttgtctg tgccacttta
    18961 caggttggga agcgggttaa aaggaacaac accatgacca caaccagaat cataatcata
    19021 tacttatacg gttcaagggt gttctctaac atcagcacct taaagttgtg aatttgcccg
    19081 gcgttcattc ctgacatctg cttttcaagg ctttccccct cggagaaaac cagatatttg
    19141 cccaataaaa taccagacgc agcaccaatc ggataaaagg tctggctgat attgagccgc
    19201 aatgtggcat aggcttttgg accgatcatt gaactgtatg tgttcgctgc agtttcaagg
    19261 aaactcaggc caatcgcaat cgcaaaaata gctgcaagga acatagtgta ggttgccata
    19321 tgtgaggcag ggaaaaaaag tgtacaacca ccaatataca gcgtcaggcc aattaaaatt
    19381 gccaccttat aactggtctt tttaatcaca agggatgctg gtattgcaat taaaaaataa
    19441 cctccataaa atgcgctctg caccaatgct gaagcaaagt tacttagcga aaatacactt
    19501 ttgaattgag tgattaatat gtcatttaat gcagctgcgc atccccatag cgggaataaa
    19561 cacgataaca aaataaactg gaacaaggga gtcttattca gatacccatc aggcatctga
    19621 atgatgtttt tatcgttcat agtgctacct ttaactgtgc aggatgatta ttcgtttaag
    19681 gttaaaaatt cattaaattg ttcaatactc ggataagatg attgcgtacc tttccctgtg
    19741 acgctgaaag cggcaaagag agcggctttt ttcaaagcgg cttcaacatc accgctttga
    19801 acataataat gggaaaagca accaataaat gcgtcaccag cgccactagt atcaacagca
    19861 tttactttga atgcaggaac atggacttcc tgatcacggg tcatccataa tgcgcctttt
    19921 tcgctcatgg taacaataat attgttcagc cctttatcaa ctaacgaacg tgcggccaaa
    19981 cgaatatgat cataagtatc aaccgacata ccggttaata tttccagttc tgtttcattc
    20041 gggataaaga aatcacattt gcaggcataa gacatatcta actcacgcaa tgccggagcc
    20101 ggatttaata acacttcaat accatttttc ttaccaaact caatcgcgtg gtaaactgtt
    20161 tccagttgaa cttccagttg taaaacgatc aatttgcatt ttttcagatc ttctgcagct
    20221 cgatcgatat cttccgggga aagaaattta ttcgctccct taattattaa tatactattg
    20281 ctcgagttgg cattaacaaa gatcggtgca acaccactgc tggtacaggg gaccttctca
    20341 acataagtgg tattaattcc ccatgattcg agattacgaa tagtattatc cgcaaaaata
    20401 tcatcaccta ctttagtcag catcaggact tttgaattca atttagccgc cgccaccgct
    20461 tgattagcac ctttcccacc acatccgatt ttgaaggcag gtgcttccag agtttctcct
    20521 tctttaggca tctgattcgt gtaagtaatg agatccacca tattggaacc aataactgca
    20581 atgtccattt cactacctct tataaacttt cgcataacaa tggtatttaa ataacattag
    20641 catgttactt ttgcatcatt tgtgactgag atcgcgatta gcacatcaac ccgatgttta
    20701 tttaatagac ttccagtctt atcactcagg ccaacactat ctaatcataa gcaacctaac
    20761 aggattaata ccgaaaattc agcagtctat acccttttca tttcaaaggg tcggtcgtat
    20821 agtatggtaa ctaaaacaat gtttactaat gccataatgt tatttttata acatttttac
    20881 ggagagagag ttgatggaaa cgaagcaaaa agagcgtatc cgacgtttga ttgaaatact
    20941 taagaaaacc gacagaatcc atttgaaaga cgcggcacga atgctggaag tttctgtaat
    21001 gactattcgt cgcgatctcc atcaggaaga tgaacctctg ccactgaccc tactgggtgg
    21061 ctatattgta atggtgcata aacccgcacc atccatgcca gtaatccagg acgttccgag
    21121 aaatcatcgt gatgacttac ctattgcaat tctggccgcc ggaatggtta atgaaaatga
    21181 tctgatcttc tttgataatg gccaggagat accgctcgtt ataagcatga tcccggatgc
    21241 aatcaccttc actggcatct gttactcaca tcgtgtcttt gttgcgttga atgaaaaacc
    21301 taatgtgaca gcaatacttt gtggtggtac gtatcgtgcc agaagtgatg ctttttacga
    21361 tgccagtaac tcttcgccat tagactctct caatccgcga aaaatattta tttccgccag
    21421 cggtgtacat gatcactttg gcgtcagctg gtttaatccc gaagatcttg ccactaagcg
    21481 taaagcgatg gcccgtggac taaggaaaat tttgctcgcc cgccacgcct tgttcgatga
    21541 agtagcctct gccagcctcg caccgctctc tgcatttgat gttctgatta gcgatcgtcc
    21601 gttaccggca gattatgtta cgcactgccg gaatgcttct gtaaaggtca ttacacctga
    21661 ttcagaagac gaatgactta ctgaaaaaac accgtactct tgttaaacat cgtcggattg
    21721 gactgattac gttgcacttt catcacatat tccagcttat caatttggct tatcatctac
    21781 tccagacgct ggtcatcatt gaccaatagt ctgatatggc ttttgtcgct gtcctgaatc
    21841 ggcaggcaga aaatgccttc aacgttaaaa gcgcgacggg aaaaagccca caagggtgag
    21901 tcattacgcc cggataataa acaggctcct ccctgttatg gaagccgttg tattgtccat
    21961 aatgtctggg tttttaatgc acactggata acaaccggac gaatcttcaa atatcactgg
    22021 cgctgacagc cggtttgaat tcatctcaca accctgcata gagcgaatct cctgcctgca
    22081 cgtcactcca ctccatggta taaacttcac actctttatc tgcggctagt ttcagtcacc
    22141 agataagcat cgactatgaa aggtgagcca tgacaacata acgttggtaa cgctctgacg
    22201 ccttaacgga agatgcctgc caccacaggg aatgtaaacg actgaagtgt ggcctttaat
    22261 gccgtgaacg gctcatggtc tcctggcacg gttgccgccc caacctgtaa caacattcca
    22321 cagtacaatg tctgtcagag ccggagcctc ccatgcctgt tgtagtaact ctaccagtta
    22381 cgaacatcct tcttcaaaag tgttgtcgta tctcgcatgg aagaaaagat cctgactaag
    22441 cagcaacaca caacgtattg cggaacctgc atgtttttcc tgtaactagt gcattacgac
    22501 atatggtaat agctacctgt gtggtttcgc tggataacaa ggggatttat tcgcaagtaa
    22561 aatgcctgat aaaatacacg aatctagtaa tcatcaatat ttactctggt cgaatgacgc
    22621 gtgaagtgga cggccaacag acgcagtcag tgctctacag cctgttgaat aaaacgctgg
    22681 atgtctcccg gctctgaaat taatgcttcg gttatttgca cgaaatgttc cttttcaaac
    22741 cctgttgaat tttgttaagc cggtgcagac acagtaaagc gcgcatcaac acctgaaagg
    22801 tacgatacaa acacaagtta caaacaataa taacggaagg acaattaatc agtgaggaat
    22861 aataactaaa ttaaccaaaa gaatgttact ggca
//
LOCUS       DAPHCV010000081        22550 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.081, whole genome shotgun sequence.
ACCESSION   DAPHCV010000081 DAPHCV010000000
VERSION     DAPHCV010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.081"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(296..1084)
                     /gene="phoH"
                     /locus_tag="RH033_003165"
     CDS             complement(296..1084)
                     /gene="phoH"
                     /locus_tag="RH033_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HDV2992314.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVRSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1710..2981)
                     /gene="efeB"
                     /locus_tag="RH033_003166"
     CDS             complement(1710..2981)
                     /gene="efeB"
                     /locus_tag="RH033_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HDV2992315.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSVPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTPESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDTNDYLGSALLRV"
     gene            complement(2987..4114)
                     /gene="efeO"
                     /locus_tag="RH033_003167"
     CDS             complement(2987..4114)
                     /gene="efeO"
                     /locus_tag="RH033_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HDV2992316.1"
                     /translation="MTINFRRNALQLSVAVLFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVADTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDKYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(4172..5002)
                     /locus_tag="RH033_003168"
     CDS             complement(4172..5002)
                     /locus_tag="RH033_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HDV2992317.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWISVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(5656..7164)
                     /gene="putP"
                     /locus_tag="RH033_003169"
     CDS             complement(5656..7164)
                     /gene="putP"
                     /locus_tag="RH033_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HDV2992318.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(7323..7532)
                     /locus_tag="RH033_003170"
     CDS             complement(7323..7532)
                     /locus_tag="RH033_003170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992319.1"
                     /translation="MMLQLCATVLNVTCVASLKMNEMLINEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            7587..11549
                     /gene="putA"
                     /locus_tag="RH033_003171"
     CDS             7587..11549
                     /gene="putA"
                     /locus_tag="RH033_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HDV2992320.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGDGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQSVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDERVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDNFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALIQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(11589..12227)
                     /gene="rutR"
                     /locus_tag="RH033_003172"
     CDS             complement(11589..12227)
                     /gene="rutR"
                     /locus_tag="RH033_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HDV2992321.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            12515..13606
                     /gene="rutA"
                     /locus_tag="RH033_003173"
     CDS             12515..13606
                     /gene="rutA"
                     /locus_tag="RH033_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HDV2992322.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYIQVLRDLWGSGKS
                     DFKGDFFTMDDCRVSPQPSVPMKVICAGQSDAGMAFSARYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIENFGERIQPLMQCRAHLPALTQEVA"
     gene            13606..14298
                     /gene="rutB"
                     /locus_tag="RH033_003174"
     CDS             13606..14298
                     /gene="rutB"
                     /locus_tag="RH033_003174"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HDV2992323.1"
                     /translation="MTTLTARPEAITFDPQQTALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSSTSFARIA"
     gene            14310..14696
                     /gene="rutC"
                     /locus_tag="RH033_003175"
     CDS             14310..14696
                     /gene="rutC"
                     /locus_tag="RH033_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HDV2992324.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            14704..15516
                     /gene="rutD"
                     /locus_tag="RH033_003176"
     CDS             14704..15516
                     /gene="rutD"
                     /locus_tag="RH033_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HDV2992325.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLDQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTVLVSVNGWLRINAHTRRCFQVREQLLHSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAACKELL"
     gene            15513..16103
                     /locus_tag="RH033_003177"
     CDS             15513..16103
                     /locus_tag="RH033_003177"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HDV2992326.1"
                     /translation="MNEAISPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRSLGLDTGPMSGFDRQHVDDAFFT
                     GSTLKSNLLINIGYGDSSKLYARLPRLSFEETCGLM"
     gene            16114..16608
                     /gene="rutF"
                     /locus_tag="RH033_003178"
     CDS             16114..16608
                     /gene="rutF"
                     /locus_tag="RH033_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HDV2992327.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCLQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            16629..17957
                     /gene="rutG"
                     /locus_tag="RH033_003179"
     CDS             16629..17957
                     /gene="rutG"
                     /locus_tag="RH033_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HDV2992328.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSIFMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKNVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAPVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNTLLSRRLVDVPPPEVVHQEP"
     gene            complement(18040..18213)
                     /locus_tag="RH033_003180"
     CDS             complement(18040..18213)
                     /locus_tag="RH033_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDV2992329.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            18586..19182
                     /gene="wrbA"
                     /locus_tag="RH033_003181"
     CDS             18586..19182
                     /gene="wrbA"
                     /locus_tag="RH033_003181"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HDV2992330.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            19203..19430
                     /locus_tag="RH033_003182"
     CDS             19203..19430
                     /locus_tag="RH033_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HDV2992331.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(19468..20709)
                     /gene="agp"
                     /locus_tag="RH033_003183"
     CDS             complement(19468..20709)
                     /gene="agp"
                     /locus_tag="RH033_003183"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HDV2992332.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYKLLEQIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDRVLNEAVK"
     gene            21244..22164
                     /gene="cbpA"
                     /locus_tag="RH033_003184"
     CDS             21244..22164
                     /gene="cbpA"
                     /locus_tag="RH033_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HDV2992333.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFSRQFQHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            22164..22469
                     /gene="cbpM"
                     /locus_tag="RH033_003185"
     CDS             22164..22469
                     /gene="cbpM"
                     /locus_tag="RH033_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HDV2992334.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPSEIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
BASE COUNT         5303 a         6050 c         5878 g         5319 t
ORIGIN      
        1 cagtcaaatt ttggctcatt gttgattaaa cctcatacgc aaaattggca ccagacctcc
       61 tgtacaggtt gaatggctgg aaaaagatag gcaaaaaact ctccgcgctg gcggcattac
      121 attggtcgcg tgctatgacg aaaaatgcct ggtaaaagta tctaaaccag aattaatttc
      181 agcttatcaa cacgctcaaa gtcgcccccc agaacgttaa cctgaagtga aagtctttgc
      241 tgttaatgaa ataaaacaaa gcccggttcg cccgggctct gcgccgataa tacacttaac
      301 tgtatgcatg tagcgtacgt tggcaaagtg ccgaacgtac gcaatcctct ttaccgaatc
      361 ggacgatccc gaccatttca tcttcttcga aacgttccag cgcgtcactt aatccggagc
      421 gcacgccgcg aggcaaatcg cattgcgtga tatcaccgtt gacgataacc gtcacgttct
      481 ccccgaggcg ggttaaaaac attttcattt gcgcggcagt cacattctgc gcctcgtcaa
      541 gaatgacgac tgcattttca aaggtacgtc cacgcatata ggcgaacggc gcaatttcca
      601 ccttccctat ttccggtcgc aggcagtact gcataaagga agcccctaag cgccgaacca
      661 gcacgtcgta gaccgggcga aaatagggag caaacttttc tgcgatatca ccaggtaaga
      721 agccaagatc ttcatcggct tgcagaactg gacgggtgac gataatcctg tcgacatcct
      781 tatgtatcag ggcctctgcc gcttttgctg cgctgatcca ggtttttccg cacccggctt
      841 cgcccgtggc gaatatcagc tgcttactct caatagcctt cagatagtgc aattgcgctt
      901 catttcgcgc gaggatgggc gaggtatcgc gactatcgcg ggccatacca atggcttcta
      961 cgccgctcat ttgcacaagc gaggtgaccg attcttcttc acgctgctta tggctgcgcg
     1021 aatcccgtct cagcacacgt tttgcctcgc gacgagcttt gatcactgct ttttgtcttc
     1081 ccatggagag caccttgagt tgtttgtatt catcacacgc gccgttggca gcgcgattat
     1141 gcgcacgaac atcagagggt tggcttcctt gtaagccata gtttgctttt ggataaaatg
     1201 ccgaaaaacg gctacgcaca ccgtttgcgg cgtcggtaac acgagtaaag agaggaagtg
     1261 agaaaaatac agagtgacga agttgctacc ggaggagaaa tctccgcgag tggtggagcc
     1321 ttgcttaaca aaacattgtc cagaacagga cgttaccatc cgcgatctcc atagtgactg
     1381 actatcactg ccgggaactt ccgctgctac ttaataatta caacagattt cgcattcatt
     1441 gcaacaatat attcacttac atttaaccat aaaacaccat ttcagtgaca ttagtttcta
     1501 ctggaaagat gacaaagtga tgacagagat gaaaaaagct gtgtgctttc agcaggattc
     1561 gcagggctgg taaaagcttg ccggatgcgg cgtgaacgcc ttatccggcc taccgacccg
     1621 caccgattgt aggcatgata agacgcgcat gcgtcgcatc agcgcacgaa cgcctggtgc
     1681 ggcacgaacg ccttatccgc ctgaaaacat taaacccgca ataacgcgct tccgagataa
     1741 tcgttcgtgt ccttcacccc cggcagcgca aaaaaataac cgccgccgat aggtttaacg
     1801 tattcctcca gcgcttcgcc attgagcctt ttttgtactg tcaggaagcc tttttccaga
     1861 tcgtgttgat agcagacaaa cagtagcccc atatccagtt gtccggagtt ggtgacgccc
     1921 aacgaataac tgtagccacg acgcagcatc agacttgact cactctctgg cgtgcgggga
     1981 ttcgccagcc ggatatggct gtccagcgca atcaccttcc cttccggatc gctggcgtaa
     2041 tcgggaacat catgctcatg ctgcattccc agcggcgcgc cggtttgctt atcacgccca
     2101 aatatcgtct gctgttcttt cagcggcgtt ctgtcccaaa attccacccg aaactgaatc
     2161 aaccgcaccg cctggtagct gccgccgatt gtccacgcag gctcctgctg atctgccgtc
     2221 acccacacca ctttttgcat caacttatca ttctggctat cgggattggc agtgccgtct
     2281 ttgaaaccca gcaaattaat cggcgtctct ttgcctttac tacgcgccgc gtgatcggaa
     2341 ataaaccctt cccgcttcca gcgcacactg agcaaatccg gcgtgtgttt gatgatatcg
     2401 cgcagcgcat ggatcaccgt atcctgggtg ttggcgcaaa tttgtagcaa cacatcacca
     2461 tgacataatg ctgcatcaag cgagtcgttg gggaaacgcg tcatcttctg caactttttc
     2521 ggcatctgtg gcgcaagacc aaaccgttca tcaaacaacg agtgacccac cgataacgtg
     2581 atggtgagat tatcgggcgc aatgtagccg ccaagaatgc cggaatcgag tggtggcagg
     2641 cgcggatttg gcgtttctgg tgccgctccg ccctgagtca gaaaagcaaa acgctgagtc
     2701 aacaagcgaa acaaccgttc aagatcggct ttatcgctgg caagcacatc aaacgccacc
     2761 agcatcattg cagcctgttg tggtgtcagg atccctgcct gatgctcacc ataaaacggc
     2821 tgtttctcat tgcgtgcatc cggtgaaagc gtacccggca cactttgcgt tttttgtgca
     2881 tgagcgaccg gacaacttcc cgccagcgcc agtgcaccaa tccctttcag taaacgtcgg
     2941 cgtgacggtt cattcacgcc gttttcatct ttatactgca taacgtttaa tccagtccca
     3001 gcacaccgcg aagttgcgcc agatcttccg ccagcgcagt aatcggtcct ttcagtgcat
     3061 tccggtcggc atcggtcaat ttgtcgtagg tttcaaaacc gtctttagta cggtatttcg
     3121 ccagaatggt atcgaccttt ttaaagttgg catcgacttt tgccaacagt tccgggttgg
     3181 ctttttgcag ttgtggacgc agtagatcga caattttctg cgagccttca acgttagcct
     3241 ggaaatccca cagatcggtg tggctgtagc gatcttcttc accgctgatt ttgctggctg
     3301 ccacttcctc aatcagtccg gctgcaccgc cgaccacttt tgaaggtggg aaagccagtt
     3361 cactgatgcg tttttgcaaa tcgaccacat cggtataaag ctggtcagcg tacttatcca
     3421 ttcctttggt ggtgttgtcg ccaaacaatg ctttttccag acggtggaaa ccggtgaatt
     3481 ttggatcggc ggctttttgc tcataatcat cttcacgggc gtcaatgcta ccatccagat
     3541 cggagaacag ttcagcaatc ggttcaatgc gctcatagtg ctggcgcgtc ggcgcataca
     3601 gtgcttttgc tttttcgata tcgcctgctt taatagcgtc ggtaaaggct ttggtgtcgg
     3661 ccaccagctg cgtggtttcc gccatgacat acgctttata tgcagtaatt gcaccaccaa
     3721 gacttaacag cgcatcactt tgcgccgcat ccgccgttgc ctcacctttg acaatcaact
     3781 tccctttcgg gttagtcagc agaccgcagg tcatatcgta ttcgccaggc tgtaaattcg
     3841 ccgtcatttt ctggctaaag ccaggggcga tattttctcg ctcttccacc accatcacgc
     3901 ctttgaggat ctcccactcc agcgccttct ggctgtggtt ctgaataatg aactgtgttt
     3961 tcccggcgtt aaccgtaatg gtcatcggtt cgcactgctt atccgtcacg gtgactttga
     4021 cttgcggcac atcagcggcg ttagccataa aagcggaaga aaacagcaca gccacactca
     4081 actgcaatgc gttacggcgg aagttaatgg tcatgacgag actatccctt taaagtatgt
     4141 tgtaactaag caagagtttg cgtcgtattt gctacgcaga gcgagacgct gtcgcccctg
     4201 cacgtggtgg cagggcaaat gccaccagcg ccgggatgag ataaataaac cagacggcga
     4261 cttcgctgac acttggcgct tcctgatagc caaaaatccc ttccatcagc gtgccaaaca
     4321 gcgagtgagt tgacagcacc gcgctcatat cgaaggcgat ttcctgaaag tggttccaca
     4381 atccggcttc atgaaatgcg cgaatggcac cagctgccag cccagcggcg acgaagagaa
     4441 taaacagact ggtccattta aaaaatgcac caagattgag gcgaataccg ccccagtaga
     4501 gcaggaagcc gagcaccacg gctgtagcaa gaccgagcat tgcacccagc ggcggccaga
     4561 tcccgacatc ttgttgaaat gccgccagca ggaaaaagac cgactccagc ccttcccttg
     4621 caacggcaaa aaagaccatc atcaccagcg cccagccatg atgatttcca cgctgcaatg
     4681 cgctatcgac tgcctgttcc agttgcactt tgacgttgcg cgacacttta cgcatccaga
     4741 aaaccatcca ggtaaggatc accacggcga tcaccgccac gataccttca aacagttcct
     4801 gttctttttg cgggaattcg ccggtggttt cgttaatgaa gatacccaag cccaggcaca
     4861 acgcagcggc aagcaacacg ccaatccaca tcacactaat ccatcggcct cgctgggtac
     4921 gcttaagata gctggcaatc aaactgacaa tcagcgcggc ttcaagtcct tcgcgcaaca
     4981 taatgagaaa cggaacaaac atgccaccca cccttaaacg tttttctgag tcaatctatg
     5041 caaagaaaag taaatcgcag tgatagcgat tatcattacg gcaaagaaaa atacaagcgg
     5101 aatgtttata aaatgatgtt ggaatgtaaa caaagagagg taatggcagg ggatataaac
     5161 gattattcca ggtacaaaga tgggattgtt tacaacgagc catgtgttct tgccagatgc
     5221 cgcgtaaacg ccttatccag ccgacaaaac tgtccagact caatgcatca gagcttccat
     5281 gtaaaactga taagcgggtg tatcaagcaa tttcgtccat ccgcgattat caaatctaac
     5341 tttttgcccg gaacataata acagctcggg tgttggtggc gtcaaagtcg ctgaagcgtt
     5401 ccccgaaggc cggggcagcc cacatggatg tgggctgagg gcgcgtctta caggacatta
     5461 cctcgcgccc gacccggtag ccgaagggga taagctgaag gtaccgcgca gcggcgattt
     5521 tgttcgccag agcccggggt gcagggggtg gcggcgattg gccgccccct gcaccattca
     5581 atttatatgg cgaggcacat taagggagcg caacccgctg ctgaagccaa acaaaaccgc
     5641 cgcaggctaa gtcccttagc tttcctgcaa ccgtgacggc ggagccgaat gatagtgcgc
     5701 atcggcctcg gcaaagcgtt tttgcatcgc cgctgacggc gctttaccca gcaaactaaa
     5761 cactacaatc ccgatactgc cgaagatgaa gcccggaata atttcgtaca gacccagcca
     5821 gccgaactgc ttccagacga taaccgtcag cgcaccgatg atcatccccg ccagcgcacc
     5881 gttgcgcgtc atgcgtgacc acatcaccga gaacagcacc accggaccaa acgccgcgcc
     5941 aaagcctgcc cacgcgtagc tcactaagcc cagcacgcgg ttttccgggt ttgccgccag
     6001 cgcaatcgcc accagcgcca ccaccagcac catcacacgc cctacccaca ccagctcttt
     6061 ctggctggcc tgtttacgca gaaacgcttt gtataaatct tcggtaatcg cgctggaaca
     6121 taccagcagc tggcaactta aggttgacat taccgccgcc agaatcgccg acagcaaaat
     6181 cccggcaatc cacgggttaa acagaatttg cgccagttcg ataaacacgc gctcggcgtt
     6241 ctggtttacc gcacctgcta ccgccgggtg ctcgttaaag taagcgatac caaagaagcc
     6301 gacagccacc gcccctgcca ggcagaggat catccaggtc atactgatac gacgcgcatg
     6361 gacaatgctg tggtgagaat ctgccgccat aaaacgcgcc aggatgtgcg gctgcccgaa
     6421 gtaacccagc ccccacccca tcagcgagat aatggcgaca aaattcaacc ctttgagcat
     6481 atccacgttt tcgatgctct tttgtttgat tacttccagc gagtcgccaa acccaccgac
     6541 gctaatgatc acgataaccg gcgtcaggat cagggcaaaa atcatcaggc tggcctgtac
     6601 agtgtcagtc cagctcaccg cgaggaaacc gccaataaag gtgtaaagga tcgtcgccgc
     6661 agcccccgcc cacagagccg tttcgtagct catgccaaag gtactttcaa acagacgcgc
     6721 gcctgccaca atgcccgaag cgcaataaat ggtgaagaac agcaaaataa ccagcgcgga
     6781 gataatgcgc aaaatgcggc ttttatcttc aaagcgcccg gtgaaataat ccggcaatgt
     6841 taaggcgtta ttgttgtatt cggtatgcac acgcaaccgc ccggccacca gcttccagtt
     6901 gatccacgcg cctaatgtca gaccaatggc gatccagctt tcggaaatcc cggaaagaaa
     6961 aaccgcgccc ggcaacccca ttaacagcca gccgctcata tccgacgcac ccgccgataa
     7021 tgccgtcacg aatggcccaa gactacgacc gcccagaata tagtcgtcga agtttttcgt
     7081 tgatcgccag gcgataaacc caatcaatat catgccaaag atatagacac aaaatgtcac
     7141 caacatcggt gtgctaatag ccatctaaag tctccaaaaa attattatcg gcaatgtcga
     7201 aacttgccgt tatatctgcc accggaacgg ggtaacagag tttatgtttt accagggcga
     7261 ccgtatcctg ccggaagcgc tggttattca caatcgattt aacacaccat ttacattaaa
     7321 ttttagtgct cagcgacact atttttcatc aggttgcact ctctcacatt ttttgcggtt
     7381 gcacctttca aaaatgttaa ctgccgcaga gaaaaagtct gagttatttt tttaatccct
     7441 gtcatatcga tttcgtttat taacatttca ttcattttta agcttgctac gcaggtcaca
     7501 tttaacacag ttgcacaaag ttgcaacatc atggatattt cacgataacg ttaagttgca
     7561 cctttcagaa caacaggagt aatggcatgg gaaccaccac catgggggtt aagctggacg
     7621 acgcgacgcg tgagcgtatt aagtctgccg cgacacgtat cgatcgcacg ccacactggt
     7681 taattaaaca ggcgattttt tcttatcttg aacaactgga aaacagcgat actctgccgg
     7741 agctacctgc gctgctttct ggcgctgcca atgagagcga tgaagcaccg actccggcag
     7801 aggaaccaca ccagcctttc ctcgactttg ccgagcaaat tttgccccag tcggtttccc
     7861 gcgccgcgat caccgctgcc tatcgccgtc cggaaaccga agcggtttcg atgctgctgg
     7921 aacaagctcg cctgccgcag ccagttgctg aacaggcgca caaactggcg tatcaactgg
     7981 ccgataaact gcgtaatcaa aaaaacgcca gtggtcgcgc aggtatggtt caggggttat
     8041 tgcaggagtt ttcgttgtca tcgcaggaag gcgtggcgct gatgtgtctg gcggaagcgt
     8101 tgttgcgtat tcccgacaaa gccacccgtg atgcgttaat tcgcgacaaa atcagcaacg
     8161 gtaactggca gtcacacatt ggtcgtagcc cgtcgttgtt tgttaatgcc gccacctggg
     8221 ggctgctgtt taccggcaaa ctggtttcca cccataacga agccagcctc tcccgctcgc
     8281 tgaaccgcat tatcggtaag agcggtgaac cgctgatccg caaaggtgtg gatatggcga
     8341 tgcgcctgat gggcgagcag ttcgtcactg gcgaaaccat cgcagaagcg ttagccaatg
     8401 cccgcaagct ggaagaaaaa ggtttccgtt actcttacga tatgctgggc gaagctgcac
     8461 tgaccgccgc agatgcacag gcgtatatgg tttcctatca gcaggcgatt cacgccattg
     8521 gtaaagcgtc taacggtcgt ggcatctatg aaggtccggg catttcaatc aagctgtcgg
     8581 cgctgcatcc gcgttacagc cgcgcccagt atgaccgggt aatggaagag ctttacccgc
     8641 gtctgaaatc actcaccctg ctggcgcgtc agtacgatat tggtattaac atcgatgccg
     8701 aagaggccga tcgtctggag atctccctcg atctgctgga aaaactctgt ttcgagccgg
     8761 aactggcagg ctggaacggc atcggttttg ttattcaggc ttatcaaaaa cgctgcccac
     8821 tggtgattga ttacctgatt gatctcgcca cccgcagccg tcgccgtctg atgattcgcc
     8881 tggtgaaagg cgcgtactgg gatagtgaaa tcaagcgcgc gcagatggac ggccttgaag
     8941 gttatccggt ttatacccgc aaggtgtata ccgacgtttc atatctcgcc tgtgcgaaaa
     9001 agctgctggc ggtgccgaat ctaatctacc cgcagttcgc aacgcataac gcccatacgc
     9061 tggcggcaat ttatcaactg gcgggtcaga actactaccc gggtcagtac gagttccagt
     9121 gcctgcacgg tatgggcgag ccactgtatg agcaggtcac cgggaaagtt gccgacggca
     9181 aacttaaccg tccgtgtcgt atttatgctc ctgtcggtac ccatgaaacg ctgctggcgt
     9241 atctggtgcg tcgcctgctg gaaaacggtg ctaacacctc gtttgttaac cgtattgctg
     9301 acacctcttt gccactggat gaactggttg ccgatccggt cactgctgta gaaaaactgg
     9361 cgcaacagga agggcaaact ggattaccgc atccgaaaat tcccctgccg cgcgatcttt
     9421 acggtgacgg gcgcgacaac tcggcagggc tggatctcgc caatgaacac cggctggcct
     9481 cgctctcttc tgccctgctc aatagtgcac tgcaaaaatg gcaggccttg ccaatgctgg
     9541 aacaatcggt cgcggctggt gagatgtcgc ccgttattaa ccctgcggaa ccgaaagata
     9601 ttgtgggctt tgtgcgtgaa gccacgccgc gtgaagtaga acaggcgctg gaaagtgcgg
     9661 ttaataacgc gccaatctgg tttgccactc ctccggctga acgcgcggcg attttgcatc
     9721 gcgctgctgt gctgatggaa agccagatgc agcaactgat tggtattctg gtgcgtgagg
     9781 ccggaaaaac cttcagtaac gccattgccg aagtgcgcga agcggtcgat tttctccact
     9841 actacgccgg acaggtgcgg gatgatttcg ctaacgaaac ccaccgtcca ttagggcctg
     9901 tggtatgtat cagtccgtgg aacttcccgc tggcgatttt caccgggcag atcgctgccg
     9961 cactggcggc aggtaacagc gtactggcaa aaccggcaga acaaacgccg ctgattgccg
    10021 cgcaagggat cgccattttg ctggaagcgg gtgtaccgcc aggcgtggtg caattgctgc
    10081 caggtcgggg tgaaaccgtg ggcgcgcaac tgacgggtga tgaacgcgtg cgcggggtga
    10141 tgtttaccgg ttcaaccgaa gtcgctacgt tactgcagcg caatatcgcc agccgcctgg
    10201 acgctcaggg tcgccctatt ccgctcatcg ctgaaaccgg cggtatgaac gcgatgattg
    10261 tcgattcttc agcactgacc gaacaggtcg tcgtggatgt actggcctcg gcgttcgaca
    10321 gtgcaggcca acgttgttcg gcgctgcgcg tgctgtgttt acaagatgaa attgccgacc
    10381 acacgttgaa aatgctgcgc ggcgcaatgg ccgaatgccg gatgggtaat ccgggtcgcc
    10441 tgaccaccga tatcggtccg gtgattgata gcgaagcgaa agccaatatt gagcgtcata
    10501 ttcagactat gcgtagtaaa ggccgtccgg tgttccaggc ggtgcgggaa aacagtgaag
    10561 atgcccgtga atggcaaagc ggcacctttg tcgccccgac gctgatcgaa ctggataact
    10621 ttgccgaatt gcaaaaggag gtctttggtc cggtgctgca tgtggtgcgt tataaccgta
    10681 accagctgcc ggaactgatc gagcagatta acgcttccgg ctatggtctg acgcttggcg
    10741 ttcatacgcg tattgatgaa accatcgccc aggtcactgg ctcggcccat gtcggtaacc
    10801 tgtacgttaa ccgtaatatg gtgggcgcag tggtcggggt gcagccattc ggcggcgaag
    10861 ggttgtccgg caccgggccg aaagcaggcg gtccgctcta tctctaccgt ctgctggcga
    10921 atcgcccgga aagtgcactg gcagtgacgc tcgcgcgtca ggatgcagag tatccggtgg
    10981 atgcgcagtt gaaagccgca ttgatccagc cgctaaatgc actgcgggaa tgggcggcaa
    11041 accgtccaga attgcaggcg ttatgtacgc aatatggcga gctggcgcag gcaggaacac
    11101 agcgattgct gccagggccg acgggtgaac gcaacacctg gacgctgctg ccgcgtgagc
    11161 gcgtgttgtg tattgcggat gatgaacagg atgcgctgac tcagctcgcc gccgtgctgg
    11221 cggtgggcag ccaggtactg tggccggatg acgcgctgca tcgtcagtta gtgaaggcat
    11281 tgccatcggc agtcagcgaa cgtattcaac tggcgaaagc ggaaaatata accgctcaac
    11341 cgtttgatgc ggtgatcttc cacggtgatt cggatcagct tcgcgcattg tgtgaagcag
    11401 ttgccgcgcg ggatggcgca attgtttcgg tgcagggttt tgcccgtggc gaaagcaata
    11461 tccttctgga acggctgtat atcgagcgtt cgctgagtgt gaataccgct gctgctggcg
    11521 gtaacgccag tttaatgacg atcggttaaa caggtagccg gaggatgtta caacctcctc
    11581 cggcatcttt aacgtggtcg aatcccctca ataattatcc gctgcacgtt ttcaaccgtt
    11641 tgattgaaaa acacctcatc gcgcaacgtc gcgcctgtca ccgcctccac ctgaggggca
    11701 aaatcggcgt aatgttgagt ggaagcccaa atcataaaaa tcaaatgctg cgggtcaatc
    11761 ggcgcgagtt tgccgctttt gacccaaccg gcaatcagcg ccgatttctc atcaattaat
    11821 gccttcaaat cgcccgtcag ttcatccatt aacagcggtg cacctgccag catctccata
    11881 cagaacaggc gcgaagcctg cggataatcg cgtgagactt ccagcttcag acggatgtac
    11941 tctttgatcg ccgccagcgg ggcgaaatct tcacgaaacg cttttaacgg tgccagccag
    12001 atatcgagaa tctgccgcag tacggctata tacagcgcct cttttgacgg aaagtaatac
    12061 agcagattgg tttttgaaac acccgccaac tccgcgatct gctccagcct tgtgccgtga
    12121 aaaccgaatt gtgaaaaagt gtccagtgct gcgctaagaa tcgctttttt cttcgcgctt
    12181 actgcgcgcg aacgcttacc cgttgttttc actgcgcctt gcgtcatgcg ctctcccctc
    12241 ttgttgattg gcaacaggat agcaaaaccc ccgcccggcc tcgaccttct gcactctcat
    12301 cgcgcacgaa tgcatgtttt atgtgcaact gttttgaccg tttagtccac tttttttcag
    12361 gcattttaga caacttaaca ttaacacatt aataacattc actttttaaa aactggcatc
    12421 cgctttgcaa acaagccatt acgcggctga aaaggatatg caggatgcag cgccccgtct
    12481 gactttcact ttacgagacg aagagaggtt gatgatgaaa attggcgtat tcgtacctat
    12541 tggcaacaac ggctggctca tttcgaccca cgcgccgcaa tacatgccga cctttgaact
    12601 gaataaagcc attgtgcaaa aagcggagca ctaccatttc gatttcgccc tgtcgatgat
    12661 caaactgcgt ggctttggcg gcaaaactga gttctgggat cacaaccttg agtcgttcac
    12721 cttgatggcg gggctggcag ccgtcacctc acgcattcag atatacgcga cagctgccac
    12781 cttaacgtta cctccggcaa tcgtcgcccg tatggccgca accatcgact ccatctctgg
    12841 cgggcgtttt ggcgtcaacc tcgtgactgg ctggcagaag cccgagtatg agcagatggg
    12901 gatctggcct ggcgatgact atttctcccg tcgttacgac tatctcaccg aatatattca
    12961 ggtgctgcgc gacctgtggg gctcggggaa aagcgatttt aaaggcgatt ttttcactat
    13021 ggatgattgt cgcgtcagtc cgcaaccgag tgtccccatg aaagtgatct gcgccgggca
    13081 aagcgacgct ggcatggcgt tctccgcccg gtatgccgat tttaacttct gtttcggcaa
    13141 aggcgtaaat acacccacgg ctttcgcccc gaccgctgcg cggatgaaac aggccgcaga
    13201 gcaaaccgga cgcgacgttg gctcttatgt gttgtttatg gtgattgccg acgaaaccga
    13261 cgatgccgct cgcgccaaat gggaacacta caaagcgggc gcggatgaag aggcgttaag
    13321 ctggctaacc gaacaaagtc agaaagatac ccgctccggt acagacacca acgtccgtca
    13381 gatggccgat cccacttcgg cggtaaacat caatatgggg acgttagtcg gttcttacgc
    13441 cagtgtcgcg cgcatgttag atgaagtcgc aagcgtgcct ggtgccgaag gcgtgctgtt
    13501 gaccttcgat gattttctgt cgggaatcga aaacttcggc gagcgcattc aaccactgat
    13561 gcagtgccgc gcccatctcc ctgcgctgac tcaggaggtg gcatgatgac gaccttaacc
    13621 gcacgaccgg aagccattac cttcgatccg cagcaaactg cgctgatcgt ggtggatatg
    13681 caaaacgctt atgccacgcc aggcggctac ttagatctcg ccgggtttga tgtctcaacc
    13741 actcgcccgg tcattgccaa cattcaaacc gccgtgaccg cagcgcgagc ggcagggatg
    13801 ttgatcatct ggtttcaaaa tggctgggat gaacagtatg tcgaggctgg cggccccggc
    13861 tcaccgaatt ttcataaatc gaacgccctg aaaaccatgc gtaagcagcc gcagctgcag
    13921 gggaaattgc tggcgaaagg ctcctgggat tatcaactgg tggatgaact ggtgccgcag
    13981 cctggcgata ttgtgctgcc gaagccgcgc tacagcggtt tcttcaatac gccgctggac
    14041 agcattttgc gcagccgtgg aatacgccat ctggttttca ccggtatcgc taccaacgtc
    14101 tgcgtcgaat cgacgctacg cgacggcttt tttctggagt atttcggcgt ggtgctggaa
    14161 gacgcaacac accaggcggg gccggaattt gcacagaaag ccgcgttgtt caatatcgaa
    14221 accttttttg gctgggtcag cgacgtcgaa acgttctgcg acgcgctttc ttccacgtcc
    14281 tttgctcgta tcgcttaagg agtttaacga tgccaaaatc cgtaattatt cctgctggca
    14341 gcagcgcacc gctggccccc ttcgttcccg gcacgctggc tgatggcgtg gtgtatgtct
    14401 ccggtacgct ggcttttgat caacataaca acgtgctgtt tgccgatgac ccaaaggcgc
    14461 aaacccgcca cgttctggaa accatccgca aggtcatcga gacggcgggt ggcacgatgg
    14521 cggatgtgac cttcaacagc atctttatta ccgactggaa aaattacgcc gcgattaacg
    14581 aaatctacgc cgagtttttc ccgggtgaca aaccggcgcg attctgcatt cagtgcggac
    14641 tggtaaaacc tgacgcgctg gtggaaatcg ccacaattgc gcatatcgcc aagtgaggcc
    14701 gcgatgaaac tttcactctc acctccccct tatgctgatg cgcccgtagt ggtgttgatt
    14761 tcgggtcttg gcggtagcgg cagttactgg ttaccgcaac tggcggtgct ggatcaggag
    14821 tatcaggtag tctgttacga ccagcgcggc accggcaata atcccgacac gctggcagaa
    14881 gattacagta tcgcccagat ggcagcggaa ctgcatcagg cgctggtagc cgcagggatt
    14941 gagcgttacg cggtggtcgg ccatgcgctc ggtgcgctgg tgggaatgca gctggcgctg
    15001 gattatcccg cgtcggtaac tgtgctggtc agcgttaacg gctggctacg aataaacgcc
    15061 catacgcgcc gctgttttca ggttcgcgaa cagttactac atagcggcgg cgcgcaggca
    15121 tgggtggaag cgcagccgtt gttcctctat cccgccgact ggatggcggc ccgcgcacct
    15181 cgccttgagg cagaagacgc gctggcactg gcgcattttc aaggcaaaaa taatttactg
    15241 cgtcgactta acgccctcaa acgcgctgac tttagtcacc atgcggatcg tatccgctgc
    15301 ccggtgcaaa tcatctgcgc cagtgatgat ctgctggtgc caacagcatg ttccagtgaa
    15361 cttcatgccg ccctgcccga tagccagaaa atggtgatgc gctatggcgg acacgcctgc
    15421 aacgtgaccg atcccgaaac gtttaatgct ctgttactca acgggcttgc cagcctgtta
    15481 catcaccgtg aagccgcctg taaggaattg ctatgaacga agccattagc ccaggtgcgc
    15541 ttagcacctt gttcaccgat gcccgcactc acaacggctg gcgggagaca cccgtcagcg
    15601 atgagacgtt acgggagatt tatgccctga tgaaatgggg gccgacatca gctaactgtt
    15661 ctccggcacg gatcgtgttt atccgcactg cagaaggaaa agaacgtctg cgcccggcgc
    15721 tttccagcgg caatctgcaa aaaaccctga ccgcgcccgt caccgctatc gtcgcctggg
    15781 acagtgaatt ttatgaacgg ttaccactac tgtttcccca cggtgatgcc cgcagttggt
    15841 ttacctccag cccacaactt gccgaagaaa cagcgtttcg caacagttcc atgcaggcgg
    15901 cttatctgat cgtcgcctgc cgctcgctgg ggctggatac cggcccgatg tcgggctttg
    15961 accgtcaaca cgtggacgac gcctttttta cgggcagcac gctgaagagc aatctgctga
    16021 ttaatattgg ctatggcgat agcagcaagc tttatgcgcg cctgccacgt ctgtcctttg
    16081 aggaaacctg cgggctgatg taaggagcaa actatgaaca ttgtcgatca acaaactttt
    16141 cgcgatgcga tgtcctgcat gggcgcggcg gtcaatatca tcaccacgga cggtccagcc
    16201 gggcgcgccg ggttcaccgc cagcgccgtc tgcagcgtga ccgatacgcc gcccacatta
    16261 ctggtgtgcc tgaatcgtgg ggcgtccgtc tggccggtat tcaatgaaaa tcgaacgctg
    16321 tgtgtaaata cacttagcgc cggacaggag ccgctttcaa acctttttgg cggcaaaacg
    16381 cccatggaac accgctttgc cgccgcccgc tggcagactg gtgtgaccgg atgcctgcaa
    16441 ctggaagaag cactggtttc gtttgactgc cgtatcagcc aggtggtgag cgtcggcacc
    16501 cacgacattc tgttttgcgc catcgaagcg attcatcgtc acgccacccc ctacgggctg
    16561 gtgtggtttg atcgcagtta tcacgcgctg atgcgccctg cttgttaacc tccttaagga
    16621 gacagctgat ggcaatgttc ggttttcctc actggcagtt aaaatcgacc tctacagaga
    16681 gcggcgtggt cgcgccggat gaacgattgc cgtttgctca gacggcagta atgggcgttc
    16741 aacacgcggt ggcgatgttt ggcgcgacgg tgttaatgcc gattctgatg gggctggatc
    16801 ccaatctttc catttttatg tcggggatcg gcacgttact gtttttcttt attaccggcg
    16861 ggcgcgtgcc cagttatctc ggttccagcg cggcttttgt cggcgtggtg attgccgcaa
    16921 cgggctttaa tggtcaggga attaatccga atatcagcat cgcgctgggc gggattatcg
    16981 cctgtgggct ggtttatacc gtcattggcc tggtggtgat gaaaatcggt acgcgctgga
    17041 ttgaacgact aatgccgccg gttgtgacgg gtgcggtagt gatggcgatt ggcctgaact
    17101 tagcgcctat cgcagtgaaa aatgtatcgg cttcggcctt tgatagctgg atggcggtaa
    17161 tgacggtgct gtgtattggg ctggtggccg tatttacccg tggcatgatc cagcgattat
    17221 tgattctcgt aggattaatt gtcgcctgtc tgctgtatgg cgtgatgacc aacgttttag
    17281 ggctgggaaa agccgtcgat tttactctcg tcagccatgc tgcctggttc ggtctgccac
    17341 atttttccac tcctgctttt aatggtcagg caatgatgct gattgcgccc gtggcggtga
    17401 ttctggtggc ggaaaactta ggtcatctca aggcggtcgc cgggatgacc ggacgcaata
    17461 tggacccgta tatggggcgg gcattcgtcg gtgatggtct ggcgacgatg ctttctggct
    17521 ctgtcggcgg tagcggcgta accacctacg ccgagaatat cggtgtgatg gccgtgacaa
    17581 aagtctactc gacgctggtg tttgttgccg ctgccgtcat cgccatgctg ttgggctttt
    17641 cgccgaaatt tggcgcgctg attcatacca ttccagcacc ggtgattggt ggtgcatcaa
    17701 ttgttgtgtt cgggctgatt gccgtcgcag gcgcaaggat ctgggtacaa aaccgtgtcg
    17761 atttaagcca gaacggtaat ttgattatgg tcgcagtaac cttagtgctg ggcgcgggtg
    17821 attttgctct cacgctgggc ggttttacgt tgggagggat cggtacagcc acctttggcg
    17881 cgattttact caatacgttg ttaagcagaa ggttggttga cgttccaccg ccagaagttg
    17941 ttcatcagga gccataaata gaaaaccgcc ggggtaaccg gcggtgatga ctttttgacg
    18001 agcttcagac aaaaatacat tcgtcattga ttagcccggc tagttgtcgc ttttgccgtg
    18061 actgctcttg ccaccttttt tacctgcttc agatgcgcgc tgcgggtcat ttttgaaatt
    18121 acccccgctg tgctgtccac cttttttacc tgcttctgat gctctttcgc ggtcttctgc
    18181 aaaattgccg gaaccgcctc gatggtttgc cattactgac ctccgtctgg gtatagacat
    18241 catattgcat agaattaacg cggcgtatgg cggctgccat attttcgcta cgtgggacta
    18301 agcttagtga actgacgcgg gcagacccgc gtttggtaat atcctgcaac agcttcttag
    18361 cgagaataga tatcatctcc aataattatc cataagccgc tcttatgatt aaaaaaacgc
    18421 gatgagataa aaatgtgtca ttttgcgaca aaattacgtg cttgtgaaag ttgttataaa
    18481 tcaaataagt ggttgtgaaa tttgcactct gaaaaggacg tcttatcttt aaataagtgg
    18541 tagcgaatcg ctacggaata gagataacac gaggagtggt tagaaatggc taaagttctg
    18601 gtgctttatt attccatgta cggacatatt gaaacgatgg cacgcgcagt tgctgagggt
    18661 gcaagcaaag tggatggcgc agaagttgtc gttaagcgtg taccggaaac catgccgccg
    18721 caattatttg aaaaagcagg cggtaaaacg caaactgcac cggttgcaac cccgcaagaa
    18781 ctggccgatt acgacgccat tatttttggt acacctaccc gctttggtaa catgtccggt
    18841 caaatgcgta ccttcctcga ccagacgggc ggcctgtggg cttccggcgc actatacgga
    18901 aaactggcga gcgtctttag ttccaccggt actggcggcg gtcaggaaca aaccatcaca
    18961 tccacctgga cgacccttgc gcatcacggc atggtgattg tccccattgg ctacgcagcg
    19021 caggaattat ttgacgtttc acaggttcgc ggcggtacgc cgtacggcgc aaccaccatc
    19081 gcaggcggtg acggttcacg ccagcccagc caggaagaac tgtctattgc tcgttatcaa
    19141 ggggaatatg tcgcaggtct ggcagttaaa cttaacggct aatcttcaac aggaggatac
    19201 gcatgccaac tcaagaagcg aaagctcatc acgtcggtga atgggcatct ctgcgcaata
    19261 cgtcgccgga aatagccgag gccattttcg aagtcgccgg gtacgacgaa aaaatggcgg
    19321 aaaagatttg ggaagaaggt agcgatgaag tgttagtcaa agcctttgct aagaccgata
    19381 aagattcgct tttttggggc gaacagacca tcgaacgtaa aaacgtttaa ccagcgactc
    19441 ccccgcttca cgcgggggag ttttctgtta tttcaccgct tcattcaata cgcgatcaaa
    19501 cttatccatt gggcaaaaac cgttggcatc tatcgggcaa ccgcttaatt ccagcgtcac
    19561 gcgctgcgca ggtgcctgca gggttaacgc atcggcatta cgtaactgtt ccgcactctg
    19621 gtacacatat tcaattttca tcaaatcgcg attggctttg ctgtcatgcc aacgctggaa
    19681 aacgattttg ccgccaatcg gcgtgcgttc gttctggtca tgcaactgat acggtttgaa
    19741 atccagcgcc gttaacagag aggcaatgtt ggagtcgtgc ccaactaata ccgtgatttt
    19801 cggtgcactg gtgcgatcgg tgaccagggc tttgtcgata taactgacca gcggtttcgc
    19861 aacattgcgc gccacttccg gtgaggtaaa caggctgtcc tggtaaccgt ttttcagctt
    19921 cgacaacacc ttccactgct ggtcagattt gatttctccc caggccacct gatccatcgg
    19981 aaaaccttcg tagtattgca aagtaaacgc atctaccagc gagttgccga ctttcagcgg
    20041 cccggaaacg cccggttctt gttgatactt cgcgctaaag gtatttttgc catctacaag
    20101 cgaacattgt tgcttctctt tacacgcagg agaatcttta tagttaacga tttgttccag
    20161 tagcttgtag ctgtcggtaa gctggagttt gctgagctct ttctccattg ccgccaccgc
    20221 ctgttcactg aatgcggcgg aatcatcggt gatcactggg ttaaaggtgg ggtccatagt
    20281 gcccattttt tcctggtgat gaacaggaat atcacaccca gggaatgcgc cagtaataaa
    20341 gaactgcgcg gtcgcgacgg tgcgttgcag actattggca taggcataaa cggtgtccgg
    20401 tggcgggcat tcccctgatt tcaccatccc ctgttctgcc agccattcac gcatataatg
    20461 gcccatatac acttcgagca cgcccccttt agtggtgagc tgcccaccgg ggacgtccca
    20521 ttcaggccat ttattcggcg tcgactgctc cagcacactg ccattgttcg ccagcggcgc
    20581 acgtaagtta tgacggctca tcataaggac ttgctgtagc tgatagcctt ccggtacggt
    20641 ttgtgcctga gcatttgaag cgagtaaaac tatccctgcc acagttgcgg cgattagcgt
    20701 tttgttcatt cctcatacct cttttgttat caataattaa agagtgtgac agaaatatgt
    20761 catttttccg ggaacaggtt cgcaaaatgg ctcgttgaac gcctgttttt ctatattccc
    20821 ctttaaaaac aggcaagttt tgacgcaaac tatgcctgat tagccccggc ctcgtttacg
    20881 ccgctaatga ttacatcagt agctataaag aaaatcagcg gcgttaccat taccaataaa
    20941 aaaagaatgt gagactaata gaaatggttt ttagcacatg tctttatcat taaattgcat
    21001 aacgatttaa atagtcgagt tgtgcattcc tttccttatt gtttaacttg taagcatgag
    21061 ccataagttc aggagggagg taaataaaat atgttcctcc caccttgagg cgacctcctc
    21121 tccgcgatga tttcaataac atattctgtg ttggcatatg aaattttgag gattacccta
    21181 cacttatagg agttacctta caggggttcc ttcaatttga gttgatttac gcgagataac
    21241 gctatggaat taaaggatta ttacgccatc atgggcgtga aaccgacgga cgatctcaag
    21301 acaatcaaga ccgcctatcg tcgactggcc cgcaaatacc atcctgatgt cagcaaagaa
    21361 ccggatgccg aagcccgctt caaagaggtc gctgaagcct gggaagtgtt aagtgatgaa
    21421 caacgtcgcg ctgagtatga tcagatgtgg caacatcgca acgatccgca atttagccgt
    21481 cagttccagc atggcgacgg tcagagtttt aacgccgaag attttgacga tatcttctcg
    21541 tcaattttcg gtcagcatgc ccgccagagc cgtcaacgcc ccgccacacg cggccacgat
    21601 attgaaatcg aagtggcggt tttcctcgaa gaaacgctta ctgagcataa gcgtaccatc
    21661 agctataacc tgccggttta taacgccttt ggcatgatcg aacaggaaat cccgaaaacg
    21721 ctgaatgtga agatcccggc gggcgtcggc aatggtcaac gcatccgcct gaaaggccag
    21781 gggacgccgg gcgaaaacgg cggtccaaat ggcgacctgt ggctggtgat tcacattgcg
    21841 ccacatccgc tgtttgatat tgtcggccag gatctggaaa tagtggtgcc ggttagcccg
    21901 tgggaagcgg cgctgggtac taaagtcacc gttccaacac tgaaagaaag cattttgctg
    21961 actatcccgc ctggcagcca ggccgggcaa cgattgcgcg ttaaaggcaa aggtctggtg
    22021 agcaaaaaac agaccggcga tctgtatgcg gtactgaaaa tcgtgatgcc gccgaaaccg
    22081 gatgaaaaca ctgccgcgct gtggcagcaa ctggcagacg cccagtcgtc ttttgatcca
    22141 cgtaaagatt gggggaaagc ataatggcta atgttacggt gacttttact attaccgaat
    22201 tttgcctgca taccggcatc tctgaagagg agttgaatga aattgtcggt ttgggggtgg
    22261 ttgaaccgag tgagatccag gagacaacct gggtatttga cgaccatgcc gccattgtgg
    22321 tgcaacgcgc ggtacgcctg cgtcatgaac tggctctgga ctggccgggg atcgcggtgg
    22381 cgctgacgtt aatggatgat attgcgcacc tgaagcagga aaaccgcctg ctgcgccagc
    22441 ggctttcccg gtttgtggct cacccgtgag tttgttgcct gatgcgacgc ttaacgcgtc
    22501 ttatcaggcc cacaaaaagc actgttcccg taggccggat aaggcgttca
//
LOCUS       DAPHCV010000082        22041 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.082, whole genome shotgun sequence.
ACCESSION   DAPHCV010000082 DAPHCV010000000
VERSION     DAPHCV010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.082"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..2739
                     /locus_tag="RH033_003186"
     CDS             <1..2739
                     /locus_tag="RH033_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDV2992335.1"
                     /translation="DSLLWNATAGAFSAKHGTNGTDSKITNLLAGTVSSDSTDAINGS
                     QLYGLADSFTSYLGGGADISDAGVLTGPTYTIGGTDYNNVGDALAAINTSFSTSLGDA
                     LLWDATAKGGDGAFSAGRGKDNTASIITNVADGAISSTSSDAINGSQLYDTSKYIADT
                     LGGDAEVNADGTITAPTYAIAGGSYSNVGDALEAIDTTLDDALLWDATANDGNGAFSA
                     AHGKDKTASVITNVANGAISATSSDAINGSQLYTTNKYIADALGGDAEVNADGSITAP
                     TYTIANAEYNNVGDALDALDDNALLWDATANDGAGAYNASHDGKASIITNVADGNIGE
                     GSTDAINGSQLFNTNMMIQQNSEIINQLAGNTSETYIEDNGAGINYVRTNDNGLAFND
                     ASASGIGATAVGYNSVAEGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIVKGSRDTSV
                     SEEGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVAT
                     TPTKYFHANSTAEDSLAVGEDSLAMGAKTIVNGNAGIGIGLNTLVLADAINGIAIGSN
                     ARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQITNVAAGS
                     ADTDAVNVGQLKVTDAQVSQNTQSITNLNTQVTNLDTRVTNIENGIGDIVSTGSTKYF
                     KTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVANEENTISVGSSTNQRRITN
                     VAAGKNATDAVNVAQLKSSEAGGVRYDTKADGSVDYSNITLGGGNGGTTRISNVSAGV
                     NNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYT
                     PGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            3151..4806
                     /gene="lldP"
                     /locus_tag="RH033_003187"
     CDS             3151..4806
                     /gene="lldP"
                     /locus_tag="RH033_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDV2992336.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            4806..5582
                     /gene="lldR"
                     /locus_tag="RH033_003188"
     CDS             4806..5582
                     /gene="lldR"
                     /locus_tag="RH033_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HDV2992337.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGEKEKIQLCFEATLSEDPDLASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDRARHARITRLPGDHNEHSREKNA"
     gene            5579..6769
                     /gene="lldD"
                     /locus_tag="RH033_003189"
     CDS             5579..6769
                     /gene="lldD"
                     /locus_tag="RH033_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HDV2992338.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLDKELPAALAPMAK
                     GNAA"
     gene            6842..7315
                     /gene="trmL"
                     /locus_tag="RH033_003190"
     CDS             6842..7315
                     /gene="trmL"
                     /locus_tag="RH033_003190"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDV2992339.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(7368..8189)
                     /gene="cysE"
                     /locus_tag="RH033_003191"
     CDS             complement(7368..8189)
                     /gene="cysE"
                     /locus_tag="RH033_003191"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDV2992340.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFENGDGI"
     gene            complement(8269..9288)
                     /gene="gpsA"
                     /locus_tag="RH033_003192"
     CDS             complement(8269..9288)
                     /gene="gpsA"
                     /locus_tag="RH033_003192"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDV2992341.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(9288..9755)
                     /gene="secB"
                     /locus_tag="RH033_003193"
     CDS             complement(9288..9755)
                     /gene="secB"
                     /locus_tag="RH033_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HDV2992342.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASTQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(9819..10070)
                     /gene="grxC"
                     /locus_tag="RH033_003194"
     CDS             complement(9819..10070)
                     /gene="grxC"
                     /locus_tag="RH033_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HDV2992343.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(10211..10642)
                     /locus_tag="RH033_003195"
     CDS             complement(10211..10642)
                     /locus_tag="RH033_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDV2992344.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            10887..12431
                     /gene="gpmM"
                     /locus_tag="RH033_003196"
                     /gene_synonym="pgmI"
     CDS             10887..12431
                     /gene="gpmM"
                     /locus_tag="RH033_003196"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDV2992345.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            12441..13724
                     /gene="envC"
                     /locus_tag="RH033_003197"
     CDS             12441..13724
                     /gene="envC"
                     /locus_tag="RH033_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HDV2992346.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRSSLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            13728..14687
                     /locus_tag="RH033_003198"
     CDS             13728..14687
                     /locus_tag="RH033_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HDV2992347.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQQLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQIDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
     gene            complement(14674..15708)
                     /gene="waaH"
                     /locus_tag="RH033_003199"
     CDS             complement(14674..15708)
                     /gene="waaH"
                     /locus_tag="RH033_003199"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HDV2992348.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPTDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLNRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITHQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(15947..16972)
                     /gene="tdh"
                     /locus_tag="RH033_003200"
     CDS             complement(15947..16972)
                     /gene="tdh"
                     /locus_tag="RH033_003200"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDV2992349.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(16982..18178)
                     /gene="kbl"
                     /locus_tag="RH033_003201"
     CDS             complement(16982..18178)
                     /gene="kbl"
                     /locus_tag="RH033_003201"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDV2992350.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            18392..19324
                     /gene="rfaD"
                     /locus_tag="RH033_003202"
     CDS             18392..19324
                     /gene="rfaD"
                     /locus_tag="RH033_003202"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HDV2992351.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            19334..20380
                     /gene="rfaF"
                     /locus_tag="RH033_003203"
     CDS             19334..20380
                     /gene="rfaF"
                     /locus_tag="RH033_003203"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HDV2992352.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            20384..21343
                     /gene="rfaC"
                     /locus_tag="RH033_003204"
     CDS             20384..21343
                     /gene="rfaC"
                     /locus_tag="RH033_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HDV2992353.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEQWRDLIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPLKSLNNLEAAAVYKEITLI"
     gene            complement(21391..>22041)
                     /locus_tag="RH033_003205"
     CDS             complement(21391..>22041)
                     /locus_tag="RH033_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDV2992354.1"
                     /translation="ALSFAAVMMTGTRAAIFTFPLMIMVILFLQHRDQKVFLFKGLSG
                     VFILLLACGLIFNKEIDRRINSLKADVISYATKNNSQSSVGARFAMVNAGIKGSPDGF
                     NWQSLEQRAEKIKALSAENNIYSGALLFLDVHMHNEIVEALSTKGKIGLLVLIMFYVA
                     MIYYCIREKKYILLVFPASIMLFGISDVITHAKPIPASWIVCLFLSISFLSKKEPQ"
BASE COUNT         5436 a         5768 c         5699 g         5138 t
ORIGIN      
        1 gattccctgc tgtggaacgc tacggcgggt gcattcagtg ccaaacacgg caccaacggt
       61 actgacagca aaatcaccaa cttactggca ggcactgtat cctctgatag cactgacgcc
      121 attaatggct cacaacttta tggcttagct gattcattta cgtcatacct tggcggtggt
      181 gctgatatca gcgatgcggg tgtattaacc gggccaacct acactattgg tggtactgac
      241 tacaataacg tcggtgatgc tctggcagcc attaacacgt catttagcac atcactcggt
      301 gacgccctac tctgggatgc gaccgccaag ggcggtgacg gtgcgtttag cgccggtcgc
      361 ggaaaagata acacggccag catcattact aatgttgcag acggtgcaat ttcctctacc
      421 agcagcgacg ctattaacgg ctcacagctc tatgacacca gtaagtacat tgccgatact
      481 ctgggtggtg acgcagaagt caatgctgac ggcacaatca ccgcaccgac ttatgccatc
      541 gctggcggca gttacagcaa cgtcggtgac gcgctggaag caatcgatac cacgctcgat
      601 gacgctctgt tgtgggatgc cacagccaat gatggtaatg gtgcatttag cgccgctcac
      661 ggaaaagata aaacagccag tgtaatcact aacgtcgcta acggtgcaat ctctgccacc
      721 agcagcgatg ccattaacgg ttcacaactg tataccacca acaagtacat tgccgatgcc
      781 ctgggtggtg acgcagaagt taacgctgat ggttctatca ctgcgccgac ttacaccatt
      841 gcaaatgccg agtacaacaa cgtcggtgac gccctggatg cgctcgacga taacgctctg
      901 ctgtgggatg caacggccaa tgacggcgca ggtgcctaca acgccagcca tgacggcaag
      961 gccagcatca tcacaaatgt tgctgatggt aacattggcg aaggcagtac cgacgccatc
     1021 aacggttctc agctgtttaa caccaatatg atgatccagc agaacagcga aatcatcaat
     1081 cagcttgccg gtaacaccag cgaaacctac atcgaagata acggtgcggg tattaactat
     1141 gtacgtacca acgacaacgg cttagcgttc aacgatgcca gcgcttcagg tattggcgct
     1201 acggctgtgg gttataactc tgttgccgaa ggcgatagca gcgtagccat cggtcagggc
     1261 agctacagcg acgttgatac cggtattgcc ctgggtagca gctccgtttc cagccgtgta
     1321 atagttaaag gttctcgtga caccagcgtg tcggaagaag gtgttgtgat tggttatgac
     1381 accacggatg gcgaactact tggtgcgctg tctattggtg atgacggtaa atatcgtcaa
     1441 atcatcaacg tagccgatgg ttccgaagcc catgacgccg ttacggttcg ccagttgcaa
     1501 aatgccattg gtgcagtcgc taccacgccg accaaatact tccacgccaa ctcaacggct
     1561 gaagactcac tggctgtcgg ggaagactcg ctggcgatgg gcgcgaaaac tatcgtcaat
     1621 ggtaatgcgg gtattggtat cggcctgaat actctggttc tggcagatgc catcaacggt
     1681 attgctatcg gttctaacgc acgcgccaac catgcaaaca gtattgcaat gggtaatggt
     1741 tctcagacca ctcgcggcgc tcagactggc tacaccgcct acaacatgga tgcaccgcag
     1801 aactctgtgg gtgaattctc cgtaggtagc gaagacggtc aacgtcagat caccaacgtc
     1861 gcagcggggt cggcggatac cgatgcggtt aacgtgggtc agttgaaagt aacggacgcg
     1921 caggtttccc agaataccca gagcattact aacctgaata ctcaggtaac gaatctggat
     1981 acccgcgtca ccaatatcga aaacggtatt ggcgatatcg taagcaccgg tagcaccaag
     2041 tacttcaaga ccaacaccga tggcgtagat gctaacgcgc agggtaaaga cagtgttgcg
     2101 attggctctg gctctattgc tgccgctgat aacagcgttg cgctgggtac tggctctgtg
     2161 gcaaacgaag aaaatacgat ctccgtaggt tcttccacta accaacgccg tatcaccaac
     2221 gttgccgctg gtaaaaatgc tactgacgcg gttaacgttg cgcagttgaa gtcttccgaa
     2281 gcaggcggcg tgcggtatga caccaaagct gacggttctg ttgattacag caacatcact
     2341 ctcggcggtg gtaatggcgg aacgactcgc atcagcaacg tttccgctgg cgttaacaac
     2401 aacgacgcgg tgaactacgc gcagttgaag caaagcgtgc aggaaacgaa gcaatacacc
     2461 gatcagcgca tggttgagat ggataacaaa ctgtccaaaa ccgaaagcaa gttgagcggt
     2521 ggtatcgctt ctgcaatggc gatgaccggt ctgccgcagg cttacacgcc aggtgccagc
     2581 atggcctcta ttggtggcgg tacttacaac ggtgaatcgg ctgttgcttt aggtgtgtcg
     2641 atggtgagcg ccaatggtcg ttgggtctac aaattacaag gtagtaccaa tagccagggt
     2701 gaatactccg ccgcgctcgg tgccggtatt cagtggtaat catccattaa cgaatgggta
     2761 aacgccacat tgcctgatgc gctacgctta tcaggcctac ggggtgattc agcactaccc
     2821 tgtaggccgg ataatgcgtt cacgccgcat ccggcaagat aaggcgcacc agatcaacaa
     2881 tctgagggca attctctgat gggaattgcc cttttcttca ttacctctct cccagaattg
     2941 gccctaccaa ttcttcattg atctgacctc tggttcacaa ttccccaatt aaaactcaca
     3001 tcaacgttgc caatacataa catttagtta actattcatt gtcattatcc ctacacaaca
     3061 caattggcag tgccactttt acacaacgtg tgacaaggag atgagcaaca gactcattac
     3121 acggcgtgtg tggactgcag gagacctgca atgaatctct ggcaacaaaa ctacgatccc
     3181 gccgggaata tctggctttc cagcctgata gcatcgcttc ccatcctgtt tttcttcttt
     3241 gcgctgatta agctcaaact gaaaggatac gtcgccgcct catggacggt ggcaatcgcc
     3301 cttgccgtgg ctttgctgtt ctataaaatg ccggtcgcta acgcgctggc ctcggtggtt
     3361 tatggcttct tctacgggtt gtggcccatc gcgtggatca ttattgcagc ggtgttcgtc
     3421 tataagatct cggtgaaaac cgggcagttt gacatcatcc gctcgtctat tctttcgata
     3481 acccctgacc agcgcctgca aatgctgatc gtcggtttct gtttcggcgc tttccttgaa
     3541 ggagccgcag gctttggcgc accggtagca attaccgccg cattgctggt cggcctgggc
     3601 tttaaaccgc tgtacgccgc cgggctgtgc ctgattgtta acaccgcgcc agtggcattt
     3661 ggtgcgatgg gtattccgat tctggtcgcc gggcaggtaa caggcatcga cagctttgag
     3721 attggtcaga tggtggggcg tcagctgccg tttatgacca ttatcgtgct gttctggatc
     3781 atggcgatta tggacggttg gcgcggaatc aaagaaacgt ggcctgcggt cgtggtggca
     3841 ggtggttcgt ttgccattgc tcaatacctc agctctaact tcattgggcc ggaactgcca
     3901 gacattatct cttcgctggt atcactgctc tgcctgacgc tgttcctcaa acgctggcaa
     3961 ccagtgcgcg tattccgttt cggtgacttg ggggcgtcac aggttgatat gacgctggct
     4021 cacaccggtt acaccgcagg tcaggtgctt cgcgcctgga caccgttcct gttcctgacc
     4081 gccaccgtaa cgctgtggag tattccaccg tttaaagccc tgttcgcttc aggcggtgcg
     4141 ctgtatgagt gggtgatcaa cattccggtg ccgtacctcg ataaactggt tgcccgtatg
     4201 ccgccagtgg tcagcgaagc gacagcgtat gccgccgtgt ttaagtttga ctggttctct
     4261 gccaccggta ccgccattct ttttgctgcc ctgctctcga ttgtctggct gaagatgaaa
     4321 ccatctgacg ctatcagcac cttcggcagc acgctgaaag aactggctct gcctatctac
     4381 tccatcggta tggtgctggc gttcgccttt atctcgaact attccggttt gtcatcaaca
     4441 ctggcgctgg cactggcgca caccggtcat gcattcacct tcttctcgcc gttcctcggc
     4501 tggcttgggg tattcctgac cgggtcggat acgtcatcta atgctttgtt tgccgctttg
     4561 caggctaccg ccgcacaaca aattggcgtt tctgacctgt tgctggttgc tgccaacacc
     4621 accggtggtg tcaccggcaa aatgatctct ccgcaatcta tcgctatcgc ctgtgcggcg
     4681 gtaggcctgg tgggcaaaga gtctgatttg ttccgcttta ctgtcaaaca cagcctgatc
     4741 ttcacctgta tggtgggcgt gatcaccacg cttcaggcct atgtcttaac gtggatgatt
     4801 ccttaatgat tgttttaccc agacgcctgt cagacgaggt tgccgatcgt gtgcgggcgc
     4861 tgattgatga aaaaaacctg gaagcgggca tgaagttgcc cgctgaacgt caactggcga
     4921 tgcaactcgg cgtgtcgaga aattcactgc gcgaggcgct ggcgaaactg gtaagcgaag
     4981 gtgtgctgct cagtcgacgt ggcggcggga cgtttattcg ctggcgtcat gacacatggt
     5041 cggagcaaaa catcgtccag ccgctgaaaa cgctgatggc cgatgatccg gattacagtt
     5101 tcgatattct ggaagcccgc tacgccattg aagccagcac cgcgtggcat gcggcaatgc
     5161 gcgccacacc tggcgaaaaa gaaaagattc agctttgctt tgaagcaacg ctaagtgaag
     5221 acccggatct cgcctcacaa gcggacgttc gttttcatct ggcgattgcc gaagcctcac
     5281 ataacatcgt gctgctgcaa accatgcgcg gtttcttcga tgtcctgcaa tcctccgtga
     5341 agcatagccg ccagcggatg tatctggtac ccccggtttt ttcacagctg accgaacaac
     5401 atcaggcagt cattgacgcc atttttgccg gtgatgctga cggggcgcgt aaagcaatga
     5461 tggcgcacct tagctttgtt cacaccacca tgaaacgatt cgatgaagat cgggctcgcc
     5521 atgcacgcat tacccgcctg cctggtgacc ataatgagca ttcgagggag aaaaacgcat
     5581 gattatttcc gcagccagcg attatcgcgc cgcagcgcaa cgcattctgc cgccgttcct
     5641 gttccactat atggatgggg gtgcatattc tgaatacacg ctgcgccgca acgtggaaga
     5701 tttgtcagaa gtggcgctgc gccagcgtat tctgaaaaac atgtctgact taagcctgga
     5761 aacgacgctg tttaatgaga aattgtcgat gccggtggcg ctaggtccgg taggtttgtg
     5821 tggcatgtat gcgcgacgag gcgaagttca ggctgccaaa gcagcggatg cgcatggcat
     5881 tccgtttact ctctcgacgg tttccgtttg cccgattgaa gaagtggctc cggctatcaa
     5941 acgtccgatg tggttccagc tttatgtgct gcgcgatcgc ggctttatgc gtaacgcact
     6001 ggagcgagca aaagcagcgg gttgttcgac actggttttc accgtggata tgccaacacc
     6061 gggagcgcgt tatcgtgatg cgcattctgg gatgagcggc ccgaacgcgg caatgcgccg
     6121 ctatttgcag gcggtgacgc atccgcaatg ggcgtgggat gtgggcctga acggtcgtcc
     6181 gcatgattta ggtaatatct cagcttatct cggcaaaccg accggactgg aagattacat
     6241 cggctggctg gggaataact tcgatccgtc catctcatgg aaagaccttg agtggatccg
     6301 cgatttctgg gatggcccga tggtgatcaa agggatcctc gatccggaag atgcgcgcga
     6361 tgcagtacgt tttggtgctg atgggattgt ggtttctaac cacggtggcc gccagctgga
     6421 cggtgtactc tcttccgctc gtgcgttgcc cgctattgca gatgcggtga aaggtgatat
     6481 cgccattctg gcggatagcg gaatacgtaa cgggcttgat gtcgtgcgta tgattgcgct
     6541 cggtgccgac accgtactgc tgggtcgtgc tttcctgtat gcgctggcaa cagcgggcca
     6601 ggcgggtgta gctaacctgc taaatctgat cgaaaaagag atgaaagtgg cgatgacgct
     6661 gactggcgcg aaatcgatta gcgaaattac gcaagattcg ctggtgcaag ggctggataa
     6721 agaattgcct gcggcactag ctccgatggc gaaagggaat gcggcatagt tgggggtgag
     6781 gggaaaaggc cgtaccaatc accaattttc tgctatcctg cccccgcact aagggggcag
     6841 tatgctaaac atcgtacttt acgaaccaga aattccgcca aatactggca acatcatccg
     6901 tctttgcgct aataccggct ttcgtctgca tatcatcgaa ccgatgggat ttgcctggga
     6961 cgataagcgc ctgcgccgcg cggggctgga ctatcacgag tttaccgccg ttacgcgtca
     7021 tcatgactat cgcgcgtttc tcgaagcaga aaatccccag cgtctgtttg ccctcaccac
     7081 gaaaggtacg cctgctcaca gcgccgtaag ctatcaggat ggcgattatc tgatgttcgg
     7141 cccggaaaca cgcggcctgc cagcgagcat tcttgatgcc ctgcccgctg aacaaaaaat
     7201 tcgcattcca atggtgccgg acagtcgcag catgaacctg tccaatgcgg tgtcggtcgt
     7261 ggtgtatgaa gcctggcggc agctgggata tccgggagcg gtgttgagag attagttact
     7321 gtatgccgga tagatgatta catcgcatcc ggcacaatca caggacgtca gatcccatcc
     7381 ccattttcaa acgtatggtt aataccgttg aaatgctgat ccatatccat tgacggttta
     7441 tcgctatccg gcttgccgac aatgcgtgcc ggaacgccag cggcggtggt atgcggcggc
     7501 accggttgca gcaccacgga acctgcgcca atcttcgcgc cgcgcccaac ttcaatattg
     7561 ccgagaattt tcgcgcccgc gccaatcatc acaccttcac gaattttcgg atgacggtcg
     7621 ccaccagatt tacccgtacc gccaagcgtc acagattgca gaatcgatac gtcgttttca
     7681 atcaccgccg tttcacccac aacgatgccc gtggcgtggt cgagcatgat accgcgacca
     7741 atttttgctg ccgggtgtat atcaacctgg aacgttacag agacctggtt ttgtagaaaa
     7801 atagccagcg cgcgacggcc ctgattccac aaccagtgac cgatgcgata cgcctgcaaa
     7861 gcatgaaaac ccttcagata tagcaacggg gttgagtatt tatccaccgc cgggtcgcgg
     7921 gtacgcactg cctgaatatc acaggccgca gaagcgatca tttccgggtc ggcggcgtag
     7981 gcttcttcca ccacttcacg gatagcaata gccggcataa tgggcgatga cagcttgttc
     8041 gccagcatgt agctcagtgc actgccaagg ttttcgtgct tgagtagcgt cgcgtggtaa
     8101 aaactggcca gcattggctc acagtccgcc agcgttctgg cttcagcttt aatattgttc
     8161 cagacaattt ccagttcttc acacgacatt gcttactcca cacgataaga taatgaccga
     8221 cccgttctgc gcgggccggg tcatagtggt aacaaaggtt ccctgggttt agtggctgct
     8281 gcgctcgtcc ttgcgtgcac gacctagtaa agtcaatgct gcctcgcgcg cgttttttcc
     8341 gcaatataat acttgataaa tttcctcggt tattggcatt tcaacgccga agcgatgcgc
     8401 cagttcgcgg acttctttcg tattgcggta gccttccacc acctgaccaa ttttctcctg
     8461 cgcgctttgt acatccatgc cctgaccgag catcatgcca aaacggcggt tacgtgactg
     8521 gttatcagta caggtaagca ccagatcgcc cagccccgcc atgcccataa aggtggcagg
     8581 atcggcaccc agcgccgcgc caagacgcga catctcagcc agcccacggg tgatcagcgc
     8641 cgtacgcgca ttcgcgccaa aaccgatacc gtcggacatc cccgcgccaa tggcaatgac
     8701 gtttttcacc gcgccgccaa gctgcacacc gatgaaatcc gggttgctgt aaacgcggaa
     8761 acttttgcca cagtgcagca attgctggag gtcatcggca aaggtctggt ccgtcgaggc
     8821 cagcgaaatg gccgtcggta aacctgccgc cagttctttc gcaaacgttg ggccagagat
     8881 aaccgccagc ggaatttgat cgcctaaggc ctcacgcgcc acgtcctgta acagacgtcc
     8941 ggtttctgct tccagccctt tggtcgccca caccagacgc gcatcaggac gcatcagcgg
     9001 tttaatctgg cgcagcactt caccaaagac atggctgggt acgacgacga gaatattacg
     9061 gctggctgcc agcgcagtgg cgagatcgct ttcaagatgg agcgtatcgg gaaaaggcac
     9121 atcggggaga aacgcggcgt tacagcggtc gcgttcaagc gttgcgatat gttcagggtc
     9181 atggccccag aggacaacct cgtggccatt tcttgccagg gtgatggcaa gagcggtgcc
     9241 gtacgagccg gcaccgatca cagtcattga agcattacgt tggttcatca ggcatcctga
     9301 tgttcttcag taccttcgcc agcctgctgc tgcaaatagt tcatgaacag cgcatcgaag
     9361 ttaaccggcg caaggttcag ttgcgggaat gtaccgcggg ataccatgct ggtgatgcac
     9421 tcacgagcat acgggaacag aatgttcggg cagtatgcgc ccaggcaatg tgccatctgg
     9481 gtgccttcga tacccgcgat ggagaaaata ccgccctgct gaacttcaca caggaacgcg
     9541 gtttcttcgc ccagggaggc cgttacggta acacgcagta ccacttcgta aacgtcatct
     9601 gccagttgag tagatgccgt atccagatca agtttaactt ctggttgcca atctttctgg
     9661 aaaacgtgcg gcgcgttcgg cgcttcgaaa gaaatatcct tggtataaat acgttggatc
     9721 tggaaagtca tttcggtgtt gttttgttct gacatgtgta gaaaaccctt aagtgttgtc
     9781 cttaaaatac tgcgcaatgt aatgccaacg ttcacacgtt atttcagcag gggatccagt
     9841 ccaccacgtg catccaatgc atacaagtca tcacagccac caatgtgctg tgcgtcaata
     9901 aaaatctgtg gaaccgtggt gcgaccgctg cgtttgatca tctcttcacg cttagcggca
     9961 ttgccatcga tcggcaactc ctgaaaactc acgcccttgc tgctcagcag tgcttttgca
    10021 cgatggcaat acgggcaggt ttctttggta tagatttcaa cattggccat aaattagctc
    10081 ctgtttttta ccctgaaatt tcatgttgca gggcggcagc aaaatggagt gtccccagaa
    10141 gcttatatca ataagtcgct gggggcctaa ttgcagctaa cggcctgcat catgaaagac
    10201 gacaggtaaa ttatttgccg cgcaccaaag gcaagttttc gccagcccag ccagcgacgc
    10261 cttctttcag tacgaatact tgcgcaaaac cagctttcgt cagcgcgttt gcaggctcct
    10321 ggcactgcat gccagaacca tctaccacga taaccggttt gtctttgtgc ttctcaagct
    10381 caccaacatt gttggctttg atttcgctcg gcaacaggtt gatagaacct gcgatatggc
    10441 ctttacggaa gtcatcacgc tgacgtaaat ccacaaccac agcgtcttct ttgttgatca
    10501 gacgcgtagc ttcaccacga gtaatcacct tcactttcga ggtcaggctc ttaaacgtag
    10561 tcacaagaac cgccaccagt aacgcgatcc aggcgatact cagtatggga tgacggccaa
    10621 caaattgcat aatttcttgc atggggggta acaactcccg acgtagtgat taaaaaaacc
    10681 aggaaaggag tatacctgcg cgttgcggca aatacagcca gcgcgttaac tggaatgcaa
    10741 ttttgcgggg cgctacgaaa aaaaagcgta aatctggcat ctgcttgccg cgagcggtcg
    10801 tatattttga tcttcagagg ctattttatc gattcagctg tagtaaaatt acgcaaattt
    10861 tgactcttga gtatgaggtt gtcgcaatgt cggtttctaa aaaacctatg gtactggtga
    10921 ttctggatgg ctatggctat cgtgaagaac agcaggataa cgccattttt agtgctaaaa
    10981 ccccggtaat ggatgcactg tgggccaatc gtccgcatac cctgatcgac gcttccggtc
    11041 tggaagtcgg tctgcctgac cgtcagatgg gtaactccga agtaggtcac gttaacctgg
    11101 gtgccggccg catcgtgtat caggacctga ctcgtctgga cgttgaaatc aaagatcgcg
    11161 ctttcttcgc taacccggta ctgactggcg cggtagataa agcgaaaaac gcaggcaaag
    11221 cagttcacat tatgggtctg ctctccgcag gtggtgtaca cagccacgaa gatcacatca
    11281 tggcgatggt agaactggca gctgaacgcg gcgcagaaaa aatctacctg cacgcattcc
    11341 ttgacggtcg cgacactccg ccgcgcagtg ctgaatcctc gctgaaaaaa ttcgaagaga
    11401 aatttgccgc actgggcaaa ggccgcgtag cgtccatcat tggtcgttac tacgccatgg
    11461 accgcgataa ccgttgggat cgcgttgaaa aagcttatga cctgctgact ctggcgcagg
    11521 gcgagttcca ggccgatacc gccgttgctg gtttgcaggc tgcttatgct cgcgacgaaa
    11581 acgacgaatt cgtgaaagcg accgttattc gtgcagaagg tcagccagac gcggcaatgg
    11641 aagacggcga tgcgctgatt ttcatgaact tccgtgctga ccgcgcacgt gaaatcactc
    11701 gtgcattcgt gaacgctgat ttcgatggct tcgcgcgtaa gaaagtggtt aacgtcgatt
    11761 tcgtgatgct gaccgaatac gccgctgaca tcaaaactgc ggttgcttac ccacccgctt
    11821 ccctggttaa caccttcggc gagtggatgg cgaaaaacga caaaactcag ttgcgtattt
    11881 ccgaaaccga aaaatatgcc cacgttactt tcttcttcaa cggtggcgta gaagaatcgt
    11941 tcaaaggcga agatcgcatt ctgatcaact caccgaaagt ggctacctac gatctgcaac
    12001 cggaaatgag ctccgcagag ctgaccgaaa aactggttgc ggcaatcaag agcggcaaat
    12061 acgacaccat tatctgtaac tatccgaacg gcgacatggt aggtcacacc ggggtgatgg
    12121 aagcggcggt taaagcggtt gaagcgctgg atcactgcgt ggaagaagtc gcgaaagcgg
    12181 ttgagtccgt gggtggacaa ctgctgatca ccgctgacca cggtaacgct gaacagatgc
    12241 gcgatccggc aacgggtcag gcacacacgg cacacaccaa cctgccagtt ccgctgattt
    12301 acgttggtga taagaacgtg aaagcggttg aaggcggcaa actttctgac atcgcgccga
    12361 ccatgttgtc gctgatgggt atggaaatcc cgcaagagat gactggtaag ccgctgttca
    12421 tcgtggaata atccctcccc atgaggggaa aggcgattaa taccatgaca cgggccgtga
    12481 aaccgcgcag gtttgcaatc aggcccatca tctacgccag cgttcttagc gctggcgtat
    12541 tgttgtgcgc cttttccgcc cacgcggatg agcgtgacca actcaaatct attcaggccg
    12601 atatcgccgc gaaagagcgc gcggtacgcc aaaagcaaca acaacgctcc agcctgcttg
    12661 ctcagttgaa aaagcaggaa gaagcgatct cggaagccac ccgtaagctg cgcgaaacgc
    12721 aaaacacgct caatcaactg aataaacaga ttgatgagat gaacgcgtcg attgccaaac
    12781 tggagcagca aaaagccgcc caggagcgca gcctcgccgc acaactggat gccgcgtttc
    12841 gtcagggtga acataccggt attcagctca ttcttagcgg tgaagaaagc cagcgtggtc
    12901 agcgtttaca ggcttatttc ggctatctca accaggcgcg acaagaaacc attgctcagt
    12961 tgaaacaaac gcgtgaagaa gtcgctatgc aacgcgccga gctggaagag aaacagagcg
    13021 agcaacaaac gctgttgtat gagcagcgcg cccaacaggc gaagctgacc caggcgctga
    13081 acgagcgtaa aaagacgctg gcagggctgg agtcttccat ccagcaaggt cagcaacagt
    13141 tgagcgagct gcgcgccaac gaatcccgtc tgcgtaacag cattgcccgt gcggaagccg
    13201 cggcgaaagc gcgtgctgaa cgagaagcgc gtgaggccca ggcggttcgc gaccgccaga
    13261 aagaagcgac gcgcaaaggc accacctaca aaccgaccga aagcgaaaaa tcgctgatgt
    13321 cccgaactgg tggcctgggt gcgccgcgtg gtcaggcatt ctggccagtt cgcgggccga
    13381 cgctgcatcg ctatggcgag cagctacagg gtgaattacg ctggaaaggt atggttatcg
    13441 gtgcctctga aggtactgaa gttaaagcca ttgccgacgg tcgggtgatt ctggctgact
    13501 ggctgcaagg ctacggtctg gtggtggtgg ttgagcatgg taaaggcgac atgagtcttt
    13561 acggctataa tcagagcgca ctggtgagcg ttggttcgca ggttcgcgcg ggccagccaa
    13621 ttgcactggt gggcagcagt ggcggtcagg gtcggccttc actctatttc gaaattcgcc
    13681 gccagggtca ggcggtcaat ccacagccgt ggttgggaag ataagttttg tttccatttc
    13741 gtcgtaacgt tcttgcattt gccgctctgt tggcgctctc ctcccccgta attgctggca
    13801 agcttgccat cgtcattgat gattttgggt atcgcccgca caacgaaaac caggtgctgg
    13861 cgatgccttc cgctatctcc gtcgctgtat tacccgattc accgcacgcc agagagatgg
    13921 caaccaaagc gcataacagc ggtcacgaag tgttgattca tctaccgatg gcaccactga
    13981 gtaaacagca gctggagaaa aatacgctac gcccggagat gagcagcgac gaaattgagc
    14041 gcattattcg tagtgcggtc aataacgtgc cctatgccgt ggggatcaac aaccacatgg
    14101 gcagcaagat gacctctaac ctgtttggta tgcagaaagt gatgcaggcg ctggagcgtt
    14161 acaatcttta cttcctcgac agcgtaacca tcggtaatac ccaggcgatg cgcgcagcgc
    14221 aaggcactgg cgtgaaggtg atcaaacgga aagtgttcct cgacgattcg caaaatgaag
    14281 cggacatccg tgtgcagttc aatcgcgcaa ttgatctggc gcgtcgcaac ggttcaacca
    14341 ttgccattgg gcatcctcac ccttcaacgg tacgcgtgct acaacagatg gtttataacc
    14401 tgccgccaga cattacgctg gtgaaagcca gcagcttgct gaatgaaccg cagattgata
    14461 cgtctacacc gccgaaaaac actgtgcccg acacaccgcg taatccgttc cgtggcgtga
    14521 agctctgcaa accgaagaaa ccgatagaac ctgtttatgc taatcgcttc tttgaagtat
    14581 taagcgaaag catcagccag agcacgctga tcgtttactt ccagcatcag tggcaaggct
    14641 ggggcaaaca gcctgaagcg gcgaagctta acgctagcgt aaattaaagg cgctacgcgt
    14701 aatgcgatgg tgcgaccgcg ttttgtcgcg ccattgccat aagcggaaag accacagtaa
    14761 cgcctgataa cccaccttca cgctgcgcac attggtaatc aggcgcttat acataccgga
    14821 agtaaatatc tcggcaatca tccgttggtg ggtaataata tccggctctt tgcgcaccgc
    14881 atggcaaaca cgcaatgctt cgtaagttat ttgctgatga aattccggat aaatcgtaat
    14941 tttgtcggca taatttcgat ttaatttctc tagcaggcgg gtaatcttaa tatagtgacg
    15001 ttgataatta aggtttttat tcccttgtct attcaaccga ctcactgacg tattatgcag
    15061 ataatattta tataacgatt gctcggtata tcgcgcacgc agcgcattaa acataaattc
    15121 tgttgtccag acaatatcct gatgatgtaa tccggcaata aatttaatgt tatttttaac
    15181 aataacatcg cggcgataaa cccccatcca gacaacgtga gtccaacggc gggaagaaag
    15241 ccccatccgc aaccaatccg ggccggttaa tacgccggtt gagcgaaggc gatcggtggg
    15301 gatggattgc caggtttctc ccgtttcacg aaaacaccag tcagcgttgc actgcgccac
    15361 gtcgaggtcg tcctctaacg ccatggtcat cagcgtttcg tacatggtgg gatacacctc
    15421 atcgtcagca tcgacaaaag cgacatattt gcccgtcgcc acctcgatcc cacgattacg
    15481 cgccaccgat gcgccagcat tcgcctgatg caacaaacga acgtgcggat agttttctgc
    15541 gtaatgcttt gctatttcaa cagaattatc cgttgaacca tcattaataa taatgatttc
    15601 cagagcagtc caggtttgcg taattaaaga ttccatacaa gtgcggaaat catcgcctgc
    15661 attatataac ggaataataa cactaagttt attggtgctg ttcatcataa attaccgacg
    15721 acctctgaag ttatcctgac gttttacagg ctttgtatta agaaactatt aagcctgtga
    15781 gatattattt ttacaggtga gagagtcact tcagggtaga gagataatga gaacagctat
    15841 ataagagatc agcgcaatta cccattcttt attttactga cagcatttta catatttaac
    15901 agattatcct caggaaaaag ggctggaata ccagcccttg tttgtgttaa tcccagctca
    15961 ggataacttt cccggactgg cccgaacgca tggcgtcaaa gcccttctgg aaatcatcga
    16021 tagagaaacg atgggtaatg atcggcgaaa gatcgaggcc agactgaatc agcgccgcca
    16081 tcttgtacca ggtttcaaac atctcacgac cgtaaatacc tttaatgaac aagcctttaa
    16141 agatcacttt ggtccagtcg atagacatat cagacggtgg aatacccagc atcgcaatac
    16201 gaccgccgtg gttcatggtg tcgagcatgg tacgaaacgc tggcggcgca ccggacattt
    16261 ccagaccgac atcaaagcct tcggtcatgc ccagttctgc catcacatca ttgaggttct
    16321 ctttagcgac gttaaccgca cgggtgatac ccattttacg cgccaactca aggcggtatt
    16381 cgttaacatc agtaatcacc acgttacgtg caccaacgtg tttcgccacc gccgccgcca
    16441 taataccaat cgggcctgca ccagagacca gcacatcctc gcccaccaga tcgaacgaca
    16501 gcgcggtatg cacggcgtta ccgaagggat caaaaatgga agccagatca tcggagatat
    16561 tgtcggggat tttgaaagcg ttgaacgccg gaatcaccag atactcggcg aagcagcccg
    16621 gacggttaac gccgacgccg atcgtgttgc ggcacagatg ggtacgaccg ccacgacagt
    16681 tgcgacagtg accgcaggtg atatggcctt cgccagaaac gcgatcgcca atcttgaagc
    16741 ctttcacttc ctgaccaata cctaccactt caccgacata ttcatggccc acgaccatcg
    16801 gcaccgggat ggttttttgc gaccactcat cccagttata gatgtgaacg tcagtcccgc
    16861 agatggctgt tttacggatt ttaatcagca gatcgttatg cccgagttcc ggtacaggaa
    16921 cgtcggtcat ccagatgccc tcttccgctt tcagtttgga taacgctttc atctcacatc
    16981 ctcaggcgat aacgcccagt tgtttaccaa tacgcgtgaa tgcttctact gcacgcgtaa
    17041 tttgctcagg ggtatgcgcc gcagacatct gggtacgaat acgcgcctga cctttcggaa
    17101 cgaccggata gaagaaaccg gtgacgtaaa tcccctcttt ttgcagctca cgagcaaatt
    17161 tctgcgctac taccgcatcg ccaagcatga ccggaataat ggcgtgatcg gctcccgcca
    17221 gggtaaagcc cgccgccgac atttgctcac ggaactgacg cgcgttcgcc cacagacggt
    17281 cacgcagctc gctacccgct tcaaccatct ccagcacttt gatggaagcg gcaacaatgg
    17341 ccggtgccag cgagttggag aacaggtacg gacgagaacg ctggcgcagc cactcaacca
    17401 cttctttgcg cgccgcggta taaccaccag aagccccgcc cagcgcttta ccaagcgtac
    17461 cggtgataat atcgacccgg cccatcacat cgcagtattc atgggaacca cgaccatttt
    17521 caccgacaaa accgaccgcg tgtgaatcat ctaccatcac cagggcatca tatttatctg
    17581 ccagatcgca aacgcctttc aggttggcaa tcacgccgtc catcgagaac acaccatcgg
    17641 tagcaatcag cacatgacgc gcaccggctt cacgcgcttc tttcagacga gcttcaagct
    17701 cctgcatatc gttgttggca tagcgatagc gtttagcttt acacagacgc acaccatcaa
    17761 taatagacgc gtggttcagt gcgtcggaga taatggcgtc ttccgcgccc aacagcgttt
    17821 caaacaggcc accgttagca tcaaagcagg aagagtagag aatcgcatct tccatcccca
    17881 ggaatgccgc cagtttctgt tcaagctctt tatggctgtc ctgagtgccg caaataaaac
    17941 gcaccgaagc catgccgaaa ccgtgagaat ccattcccgc ctttgccgcc gcaatcagat
    18001 ccggatgatt cgccagcccg agatagttgt tggcacaaaa gttaatgacg tggcttccat
    18061 cagccacagt gatatctgct tgctgcgcag acgtaataat gcgctcttct ttaaacaacc
    18121 cttccgcccg tgcggtttcc agatcgttgg ttaactgctg ataaaattct ccacgcattg
    18181 cgattctcca gacagggcaa attccagcac atattaccca aacttatagg tcgcgacgag
    18241 atgacgcgtt aacacttctg caaaattcag gataaataac gtccagcccc ctcacttttg
    18301 gtgaatggct gaagccaacg tcatgtagtg atatgataga agtattcgtg tctgagattg
    18361 tctctgactc cataattcga aggttacagt tatgatcatc gttaccggcg gcgcgggctt
    18421 tatcggaagc aacatcgtta aagccctgaa tgataaaggc atcaccgata ttctggtggt
    18481 ggacaacctg aaagacggca ccaagtttgt gaacctggtg gatctggata tcgcggacta
    18541 tatggataag gaagacttcc tgatccagat tatggctggc gaagagttcg gcgatgtcga
    18601 agcgattttc cacgaaggcg cgtgctcttc caccaccgag tgggacggca agtatatgat
    18661 ggataacaac tatcaatact ccaaagagct gctgcactac tgtctggagc gcgaaatccc
    18721 gttcctgtat gcctcttccg cagccactta cggtggacgc acctccgact ttattgaatc
    18781 ccgcgaatat gaaaaaccgt tgaatgtcta cggttactca aaattcctgt ttgatgaata
    18841 tgttcgccaa atcctgcctg aagcgaactc acagattgtt ggcttccgtt atttcaacgt
    18901 ttatggaccg cgtgaaggcc ataaaggcag catggcgagc gtcgctttcc atctcaacac
    18961 ccagcttaat aacggtgaat caccgaagct gttcgaaggt agcgagaact tcaaacgtga
    19021 cttcgtctat gtgggcgatg tggcagatgt aaacctgtgg ttcctggaaa atggcgtttc
    19081 cggcatcttc aacctcggca ccggtcgtgc ggaatccttc caggcagtag cagatgctac
    19141 gcttgcttat cacaagaaag gccaaatcga atacattccg ttcccggata aactgaaagg
    19201 ccgctaccag gcgttcactc aggcagatct gacgaatctg cgcgcggcgg gttacgataa
    19261 accgttcaaa accgttgccg aaggcgtaac ggaatacatg gcctggctga atcgtgacgc
    19321 ataagagctc tgcatgaaaa tactggtgat cggcccgtca tgggtgggcg acatgatgat
    19381 gtcgcaaagt ctctatcgca cgctccaggc gcgctatccc caggcgataa tcgacgtgat
    19441 ggcaccggca tggtgccgtc cattattatc gcggatgccg gaagttaacg aagctattcc
    19501 tatgcctctc ggtcacggag cgctggaaat cggcgaacgc cgcaaactgg gtcatagtct
    19561 gcgtgaaaag cgctacgacc gcgcctacgt cttacctaac tccttcaaat ccgcattagt
    19621 gcctttcttc gcgggtattc ctcatcgcac cggctggcgc ggcgagatgc gctacggttt
    19681 actcaacgat gtacgcgtgc tcgataaaga agcctggccg ctaatggtgg aacgctatgt
    19741 ggcgctggcc tatgacaaag gcattatgcg taccgcacaa gatctgccgc agccattgtt
    19801 atggccgcag ttgcaggtga gcgaaggtga aaaatcatat acctgtaatc aattttcact
    19861 ttcatcagaa cgtccgatga ttggcttttg cccgggtgcg gagtttggtc cggcaaaacg
    19921 ctggccacac taccactatg cggagctggc aaagcagctg attgatgaag gttatcaggt
    19981 ggttctgttt ggctcggcga aagatcatga agcgggcaat gagattcttg ccgctttgaa
    20041 taccgagcag caggcatggt gtcgtaacct ggcgggggaa acacagcttg atcaagcggt
    20101 tatcctgatt gcagcctgta aagccattgt cactaacgat tctggcctga tgcacgttgc
    20161 ggcggcgctc aatcgtccgc tggttgccct gtatggtccg agtagcccgg acttcacacc
    20221 gccgctatcc cataaagcgc gcgtgatccg cctgattacc ggctatcaca aagtgcgtaa
    20281 aggtgacgct gcggagggtt atcaccagag cttgatcgac attactcccc agcgcgtact
    20341 ggaagaactt aacgcgctat tgttacaaga ggaagcctga cggatgcggg ttctgatcgt
    20401 taaaacatcg tcgatgggcg atgttctcca tacgttgccc gcactcactg atgcccagca
    20461 ggcaatcccg gggattaagt ttgactgggt ggtggaagaa gggttcgcac aaattccttc
    20521 ctggcacgcc gctgttgagc gagttattcc tgtggcaata cgtcgctggc gtaaagcctg
    20581 gttctcggcc cccataaaag ctgaacggaa agcgtttcgt gaagcgctac aagcagagaa
    20641 ctatgacgca gttatcgacg ctcaggggct ggtaaaaagc gcggcgctgg tgacgcgtct
    20701 ggcgcatggc gtaaagcatg gaatggactg gcaaaccgct cgcgaacctt tagccagcct
    20761 gttttacaat cgtaagcatc atattgcaaa acagcagcac gccgtagaac gcacccgcga
    20821 actgtttgcc aaaagtctgg gctatagcaa accgcaaacc cagggcgatt atgctattgc
    20881 acagcatttc ttgacgaacc tgcctacaga tgctggcgaa tatgccgtat ttcttcatgc
    20941 gacgacccgt gatgataaac actggccgga agaacaatgg cgagatttga ttggtttact
    21001 ggcagattca ggaatacgga ttaaacttcc gtggggcgcg ccgcatgagg aagaacgggc
    21061 gaaacgactg gcggaaggat ttgcttatgt tgaagtattg ccgaagatga gtctggaagg
    21121 cgttgcccgc gtgctggccg gggctaaatt tgtagtgtcg gtggatacgg ggttaagcca
    21181 tttaacggcg gcactggata gacccaatat cacggtttat ggaccaaccg atccgggatt
    21241 aattggtggg tatgggaaga atcagatggt ttgtagggct ccgctaaaga gtttaaataa
    21301 tttagaagca gcggctgttt ataaagaaat tacattgatt taataaatag taagtgggca
    21361 ttatacccac ttacttaatc aacattttta ttattgtggt tcttttttac tcaaaaacga
    21421 tatagacagg aagaggcaca caatccacga tgccggtatt ggtttagcat gcgtaatgac
    21481 atcactaatg ccaaataaca taatagaagc agggaaaacc aaaagtatat attttttctc
    21541 ccggatacag tagtagatca ttgcaacata gaacataata agaacaagaa gaccaatttt
    21601 ccctttcgtg gaaagagctt caacaatttc attatgcata tgtacatcaa ggaataataa
    21661 agctccacta taaatattat tttctgcact taatgctttt atcttttcag cacgctgctc
    21721 aagagattgc caattaaaac catcaggact tcctttaatt cctgcattaa ccattgcaaa
    21781 acgtgctccg accgaggatt gactgttatt ttttgtagca tatgaaataa catcagcttt
    21841 taatgaatta attcgcctat caatttcctt attaaatatc aaaccacaag caagtagcaa
    21901 tataaaaaca ccactcaatc ctttaaataa aaacactttt tgatcacgat gctgaagaaa
    21961 taatatgacc ataatcataa gtgggaatgt gaaaattgcc gcacgcgttc ctgtcatcat
    22021 tactgctgca aagcttaaag c
//
LOCUS       DAPHCV010000083        22028 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.083, whole genome shotgun sequence.
ACCESSION   DAPHCV010000083 DAPHCV010000000
VERSION     DAPHCV010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22028
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.083"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(313..465)
                     /gene="hokE"
                     /locus_tag="RH033_003206"
     CDS             complement(313..465)
                     /gene="hokE"
                     /locus_tag="RH033_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HDV2992355.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            907..2025
                     /locus_tag="RH033_003207"
     CDS             907..2025
                     /locus_tag="RH033_003207"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HDV2992356.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKMGASSAIEAL
                     HHQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            2091..2339
                     /locus_tag="RH033_003208"
     CDS             2091..2339
                     /locus_tag="RH033_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HDV2992357.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            2404..2787
                     /locus_tag="RH033_003209"
     CDS             2404..2787
                     /locus_tag="RH033_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDV2992358.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRLRPDSTVNQ"
     gene            2866..3519
                     /gene="nfsB"
                     /locus_tag="RH033_003210"
     CDS             2866..3519
                     /gene="nfsB"
                     /locus_tag="RH033_003210"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HDV2992359.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAADNYVFNERKMLDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDEEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            3627..4874
                     /gene="ybdG"
                     /locus_tag="RH033_003211"
     CDS             3627..4874
                     /gene="ybdG"
                     /locus_tag="RH033_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HDV2992360.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIFLHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4942..6318)
                     /gene="pheP"
                     /locus_tag="RH033_003212"
     CDS             complement(4942..6318)
                     /gene="pheP"
                     /locus_tag="RH033_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HDV2992361.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(6420..9563)
                     /gene="cusA"
                     /locus_tag="RH033_003213"
     CDS             complement(6420..9563)
                     /gene="cusA"
                     /locus_tag="RH033_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HDV2992362.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDTMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(9575..10798)
                     /gene="cusB"
                     /locus_tag="RH033_003214"
     CDS             complement(9575..10798)
                     /gene="cusB"
                     /locus_tag="RH033_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HDV2992363.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPTEKTPTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKSPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWITAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNTDEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(10814..11146)
                     /gene="cusF"
                     /locus_tag="RH033_003215"
     CDS             complement(10814..11146)
                     /gene="cusF"
                     /locus_tag="RH033_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HDV2992364.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KTIDLESKKITIHHDPITAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(11170..12552)
                     /gene="cusC"
                     /locus_tag="RH033_003216"
     CDS             complement(11170..12552)
                     /gene="cusC"
                     /locus_tag="RH033_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HDV2992365.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLNQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSNNLKGDSASTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLPSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARTLYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            12709..13392
                     /gene="cusR"
                     /locus_tag="RH033_003217"
     CDS             12709..13392
                     /gene="cusR"
                     /locus_tag="RH033_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HDV2992366.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            13382..14824
                     /gene="cusS"
                     /locus_tag="RH033_003218"
     CDS             13382..14824
                     /gene="cusS"
                     /locus_tag="RH033_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HDV2992367.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLANSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTMMMPGHGHGHMEHSNWRMISLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLVMTASIISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVHYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     TGETIVVRCQTVDHLVQVTVENPGTPIAPEHLPRLFDRFYRIDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIILPA"
     gene            14974..17211
                     /gene="nrfB"
                     /locus_tag="RH033_003219"
     CDS             14974..17211
                     /gene="nrfB"
                     /locus_tag="RH033_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HDV2992368.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMINRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAVEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNPEQVAQLES
                     ENEGE"
     gene            17198..20170
                     /gene="nfrA"
                     /locus_tag="RH033_003220"
                     /pseudo
     CDS             17198..20170
                     /gene="nfrA"
                     /locus_tag="RH033_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            20171..21061
                     /locus_tag="RH033_003221"
     CDS             20171..21061
                     /locus_tag="RH033_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HDV2992369.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFIH
                     QKQSSAALESYLNRLLAADLQQARLWSAVPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAQSILEY"
     gene            21719..>22028
                     /gene="ompT"
                     /locus_tag="RH033_003222"
     CDS             21719..>22028
                     /gene="ompT"
                     /locus_tag="RH033_003222"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HDV2992370.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     D"
BASE COUNT         5389 a         5858 c         5496 g         5285 t
ORIGIN      
        1 aattgttcag gtttgggtag gtagccccag ggttgataga aatatcgtcc tggggcttca
       61 tctttccaga cctcagagtt aacctgaaac cggaaagctt caggcacccg ccgttatctt
      121 acgcttcctc agcaaaataa ctgtttattt atacagtgaa tcctgtttcg gacttctgtt
      181 ggcaaaactg atcattgccc ttaaaacgac atcgcccgca gccatcactg accgcgggcg
      241 aaagtgtaaa gcaggtgcct taccatcctg acctgacaac cggatatgcg gggattactc
      301 cccgcacaac ggctacttct tcggttcgta agcgagaaca gccttaaact caatattacg
      361 ttccttcacc gtaaactcac acagcgagtc tccgaccaga agcgtaaatc ccagcaccgt
      421 caaacacagc actatgactg ccacaagggc atatttcgtc agcatgttta tattgcctcc
      481 atgcgaagaa gggactacca tccaaattgt ttgggtttgg agtggcagcc ccaggattga
      541 tagaaatatc tcctggggct tcatctttcc agacctcaga gtaaacctga aaccggaaag
      601 cttcaggcac ccgccgttat cttacccttc cttagcaaaa cagctgttta tttatacagt
      661 aagtcctgtt tcggacttct caatcctcat ttgatcaact gaagctgaac ccgttttatt
      721 ctgcatgcct caatcgtcaa aattcttcgc caccagtccg cactgccata acttgtcata
      781 ttgcaaaatg tagccaattt gcttcagtca ccatcatcaa aaaaagaaac attccagaac
      841 ttccgcatct cgcccagccg ctccgacttc tatactgaat agaaaacgcc aacataagag
      901 aaacctatgc cattacccga ttttcatgtt tctgaacctt ttaccctcgg tattgaactg
      961 gaaatgcagg tggttaatcc gccgggctat gacttaagcc aggactcttc aatgctgatt
     1021 gacgcggtta aaaataagat cacggccgga gaggtaaagc acgatatcac cgaaagtatg
     1081 ctggagctgg cgacggatgt ttgccgtgat atcaaccagg ctgccgggca attttcagcg
     1141 atgcagaaag tcgtattgca ggcagccgca gaccatcatc tggaaatttg cggcggtggc
     1201 acgcacccgt ttcagaaatg gcagcgtcag gaggtatgcg ataacgaacg ctatcaacga
     1261 acgctggaaa actttggtta tctcattcaa caggcgaccg tttttggtca gcatgtccat
     1321 gttggttgcg ccagtggcga tgatgccatt tatttgctgc acggcttgtc acggtttgtg
     1381 ccgcacttta tcgccctttc cgccgcgtcg ccatatatgc aaggaacgga tacgcgtttt
     1441 gcctcctcac gaccgaatat tttttccgcc tttcccgata atggcccgat gccgtgggtc
     1501 agtaactggc aacaatttga agccctgttt cgctgcctga gttacaccac gatgatcgac
     1561 agcattaaag atctgcactg ggatattcgc cccagtcctc attttggcac ggtggaggtt
     1621 cgggtaatgg ataccccgtt aactcttagc catgcggtaa atatggcagg attgattcag
     1681 gctaccgccc actggttact gacggaacgc ccgtttaaac atcaggagaa agattacctg
     1741 ctgtataaat tcaaccgttt ccaggcctgc cgctatgggc tggaaggcgt cattaccgat
     1801 ccgcacactg gcgatcgtcg accattgacg gaagacacct tgcgattgct ggaaaaaatc
     1861 gccccttcgg cacataaaat gggtgcatcg agtgcgattg aggccctgca tcaccaggtc
     1921 gtcagcggtc tgaatgaagc gcagctgatg cgcgatttcg tcgccgatgg cggctcgctg
     1981 attgggctgg tgaaaaagca ttgtgagatc tgggctggtg actaagccgg gaattgcctt
     2041 acagaagaat atccctgaca atggtgtttt aacttacatt taacaatgtt atgaaacacc
     2101 ctttagaaac cttgaccacc gcagcaggca ttttgctgat ggctttcctc tcttgcctgc
     2161 tgctgcccgc ccccgcactg gggctgacgc tggcacaaaa actggtgacc atgttccatc
     2221 tgatggatct tagccagctt tacactttat tgttttgtct gtggttttta gtgctgggcg
     2281 ctattgagta ttttgttctg cgctttatct ggcgacgctg gttctcgctg gcggattaaa
     2341 cgtcgattat cgccgaactt cgcggcatac tttgcttatt ctccttcgcc taaaggaacg
     2401 tttatggata agcaatcact gcacgaaacg gcgaaacgcc tggctcttga gttacccttt
     2461 gtcgagcttt gctggccttt tggcccggag ttcgatgttt ttaaaattgg cggcaagatt
     2521 tttatgctgt cgtcggagct gcgcggcgtc ccctttatca atctgaagtc cgatccacaa
     2581 aaatccctgc taaatcagca aatataccca agcatcaagc cagggtatca catgaataaa
     2641 aagcactgga tttcggtgta tcccggcgag gaaatctccg aagcgttact tcgcgatctg
     2701 atcaacgatt cgtggaatct ggtggttgat ggtctggcta aacgcgatca aaaacgactg
     2761 cgtcctgact caaccgtaaa tcaatagcgc atttttctcg cttaccattt catctcgatc
     2821 cttgtaatct gctggcacgc aaaattactt tcacatggag tctttatgga tatcatttct
     2881 gtcgccttaa agcgtcattc cactaaggca tttgatgcca gcaaaaaact taccccggaa
     2941 caggccgagc agataaaaac gctactgcaa tacagcccat ccagcaccaa ctcccagcca
     3001 tggcatttta ttgttgccag cacggaagaa ggtaaagcgc gtgttgccaa atccgctgcc
     3061 gataattacg tattcaacga gcgtaaaatg cttgatgcct cgcacgtcgt ggtgttctgt
     3121 gcaaaaaccg cgatggacga tgcctggctg aagctggttg ttgaccagga agatgccgat
     3181 ggccgctttg ccacgccgga agcgaaagcc gcgaacgata aaggtcgcaa gttcttcgcc
     3241 gatatgcacc gtaaagatct gcatgatgat gcagagtgga tggcaaaaca ggtttatctc
     3301 aacgtcggca acttcctgct cggcgtggcg gcgctgggtc tggacgcggt acccatcgaa
     3361 ggttttgacg ccgccatcct cgacgaagaa tttggtctga aagagaaagg ctacaccagt
     3421 ctggtagttg ttccggtggg tcatcacagc gttgaagatt ttaacgctac gctgccgaaa
     3481 tctcgtctgc cgcaaaacat caccttaacc gaagtgtaat tctctcttgc cgggcatctg
     3541 cccggctatt tcccctcaga ttctcctgat ttgcataacc ctgtttcagc cgtcatcata
     3601 gtctgctgtt gtataaagga gacgttatgc aggatttaat atcccaggtt gaagatttag
     3661 cgggtattga gatcgatcac accacctcaa tggtgatgat tttcggtatt atttttctga
     3721 ccgccgtcgt ggtgcatatt tttttgcatt gggtggtact gcggaccttc gaaaaacgcg
     3781 ccatcgccag ttcacggctt tggttgcaaa tcattaccca gaataaactc ttccaccgtt
     3841 tagcttttac cctgcagggg attatcgtca atattcaggc ggtattctgg ctacaaaaag
     3901 gcaccgaagc ggcagatatt ctgactacct gcgcgcagtt gtggatcatg atgtatgcgc
     3961 tgctttcagt cttctcgttg ctggatgtga ttttgaatct ggcgcagaaa ttcccggcgg
     4021 catctcagtt accgctgaaa gggatatttc aggggattaa actgatcggc gcgattctgg
     4081 tcggcatttt gatgatctcg ctgctgattg gtcagtcgcc agcgattctg atcagcggtc
     4141 ttggtgcaat ggctgccgtg ctgatgttgg tatttaaaga tccgattctt ggtctggtgg
     4201 caggtattca gctttccgcg aacgatatgc tgaaactggg cgactggctg gagatgccga
     4261 aatacggcgc ggatggcgcg gtgatcgata ttgggttaac caccgtcaaa gtacgtaact
     4321 gggacaatac cattaccact attcccacct ggtctctggt ttctgactcc tttaaaaact
     4381 ggagcgggat gtcagcatct ggcgggcgac gtattaagcg cagtatcagt attgatgtca
     4441 ccagtattcg ttttcttgat gaagacgaaa tgcaacgtct gaataaagcg catttgttaa
     4501 agccttattt aaccagccgc catcaggaaa ttaatgagtg gaatcgccag caaggttcta
     4561 cggagtcggt attaaacctg cgccgaatga ccaatattgg aacctttcgt gcctatctga
     4621 acgaatatct gcggaatcat ccgcggattc gcaaagatat gaccttaatg gtgcgccaat
     4681 tagctccggg cgataacggt ttaccgcttg agatctacgc gtttactaat accgtggtgt
     4741 ggctggaata tgaaagcatt caggctgata tattcgatca catatttgcg attgtcgaag
     4801 agtttggtct gcgacttcat cagtcgccaa ccggcaatga tattcgctct ctggcgggtg
     4861 catttaagca gtaattaaaa aaaacgctct catcgaatgg atgagagcgg acatcgtttt
     4921 gtcggatgta gcgtgaatgc cttatttccg acgcagcgtt ttaaatgcca caaacaggaa
     4981 tacaatccac accggcagca ggatcgctga caagcgcata tcatccatcg tgcacatcag
     5041 cagcaaaatc atgccgagga aggcaatgca aagatagttg ccgaacggat aaagcagcgc
     5101 cttaaactgt gtttcacgtc cctgacgtcg catcgccgcg cgaaaacgca gatgcgccag
     5161 gcagatcata atccagttca acagcagcgt tgctaccacc agcgccatca gcagaccaaa
     5221 cgctttttgc ggcagcagat agttgattaa caccaccagc gaagtgatgg ctccggaaag
     5281 catcagcgag ttaatcggca cgccgcgacg gctgacgcga gtcaaaaact tcggtgcatt
     5341 accctgcaca gaaaggccaa acagcatgcg gctgttagaa taaaccccgc tgttatacac
     5401 cgataacgat gccaccagaa tgacgaagtt cagcgcagaa gccaccacgt tgctgtcgag
     5461 attatggaaa atcatcacaa atgggctact gttggatttc acttccaccc acggatagag
     5521 tgccagtaaa accaccagtg aaccgatata aaacagcaag atgcgataca ccacctgatt
     5581 cactgcttta gggatgcttt tttccggatc gcgcgcttca gcggcagtaa tcccaatcag
     5641 ctccagaccg ccgaaagaga acataattac cgccagcgac aaaatcagcc cattccagcc
     5701 ggtggcaaaa aaaccaccgt aacgccagag gttatcgata ctggctttct cgccgccgtg
     5761 accagaaaac agcagccaca ggccaaagcc gatcataccg atgatcgcca gcactttaat
     5821 cagcgcaaac cagaattcgg tttcgccata taagcgcacg ttcaccaggt taacggcgtt
     5881 gatgataata aagaaggcgg cagcccaaat ccacgttgga acatccggga accagtactg
     5941 catatagatg cccgcagcgg tcagctctgc cattcccacc agcacgaaca ttacccagta
     6001 gttccagcca gagaggaagc ccgcaaacgg tccccagtat ttataggcaa agtgggcaaa
     6061 tgaaccagat accggctcct caaccaccat ttcgccaagc tggcgcataa tcaggaaagc
     6121 gatgatcccg gcgacgccgt agcccagcaa tacagccgga cccgccatct gaatcgccgg
     6181 accaatgcca agaaacagac cagtaccaat tgcgccaccc agcgcaatca gttgaatatg
     6241 acggttatgt aatccgcgat gaagcgtcgg ctcttgattc gacgcagtat cttccgatac
     6301 ggttgacgcg tttttcacgc ctttcccctg tgtgtctttt ttgttgaggg gcacctttta
     6361 acatttagtg cccatcgtcg caagacacaa tccacacggt taaaccgggt atcctgcttt
     6421 tatttccgta cccgatgtcg gtgcagccac atcagcttat acgccgccgg gataataaac
     6481 agcgatagca aaggcgcggt gatcatgccg ccaatcatcg gcgcggcaat ccggctcatc
     6541 acctctgaac ctgctcccgt tccccacaga atcggcagca gaccagcgat aatcaccgcc
     6601 accgtcatcg ctttcgggcg cacgcgcagg accgcgccgt gatacaacgc ctcatccagc
     6661 ttctgctcgc tgaatgtttg cggattattc aacgacggct cggcctctat ggcgtgacgt
     6721 aaatacatca gcatcaccac gccaaattcg gcagcaaccc cggcaagggc aataaaaccg
     6781 gtacccgtcg ccacggaaag atgaaagccc atccaccaca gcaaccagat gccacccacc
     6841 agcgcaaacg gtacactgct gataatcagc aacgcttcgc ccacccgacg gaacgccaga
     6901 tacaacagca cgaagatgat catcaacgtc atcggcacca tgagtttcag cttatggttg
     6961 gcgcgctcca gtagctcgaa ctgcccggag aatgccacgc tggtgcccgg ttttaactgc
     7021 actttttcag ctatcgcttt ttgcagatcg tgaaccaccg acaccatgtc acgatcgcgg
     7081 gcatcgatat aaatccagct cgtcgggcgc gcattctcgg ttttcagcat cgacggtccg
     7141 gtagagactt taacgtcagc cacgtctgcc agggtgattt gctgcttcat cggcgtgagg
     7201 atcggcaact ggcgcagtgc ctgcgggcta tcgcgccagc tttgcggata acgcagatta
     7261 attggataac gagcaatccc ttccaccgtt tcgccaacca tcgccccgcc caccgcagaa
     7321 gtcacaaaca actgcacatc cgccaccgtc ataccgtaac gcgcggcttt ttcacggtta
     7381 atctcaacgt tgatataacg cccaccttcc agacgctcag caagcgcaga agccacgcct
     7441 ggcaccgttc gcgccacttc ttcaatttgc tccgccatcg tgtcgatatc cgccagcaca
     7501 gtgccggaaa ctttaatgcc aatagggctt ttaatgccag tcgagagcat atcgatacgg
     7561 ttacgaattg gcggcaccca cagattcgcc agccccggca gacgcacggt gttatccagt
     7621 tcctcaatga ttttgtccat cgtcatgcca ggccgccact gatcctgcgg cttaagctgg
     7681 atagtcgttt ctaccatctc cagtggagct gaatcggtgg cggtttccgc tttcccggtt
     7741 ttgccaaata cccgcgccac ttccggtacg ctcataatca gcttgtcggt tttttgcagc
     7801 atgctcgccg cctctgctgc ggaaatcccc ggcagcgtcg atggcatata caacaagtcg
     7861 ccttcattga tctgcggtaa aaattccccg ccaactttat tgagcggcca gagaaccgtc
     7921 agcaccgaaa gcgctgccac caacagcgtg gttttcggcc agtgcagtac tttcagcaac
     7981 agcggatgat aaacacgaat caaaaagcga ttgagcgggt tactgctttc cggcggaatt
     8041 ttgccacgga tccagtagcc catcaggatc gggatcacta cgatcgccag tagcgccgca
     8101 cccgccatcg cataggtttt ggtgaacgcc agcgggccaa acagacgccc ttcctgccct
     8161 tccagggtga agatcgggat aaacgacagc gtgataatca gcagactgat aaacagcgcc
     8221 ggccccactt caacagacgc atcggtaatc acctgccagc gcgttttatt atccagcgtg
     8281 gcgtcaggat gctggtgctg ccactcttcc agccgtttat gcgcattctc gatcatgacg
     8341 atagcggcat cgaccatcgc cccgacggca atcgcaatgc cgcccagcga cataatattg
     8401 gcgttcagtc cctgaaagtg catgacaata aaagcaatac acaaccccag cggcaacgaa
     8461 ataatcgcca ccagcgccga gcgcacatgc cagagaaaca gcgcacagac taccgccacc
     8521 acaataaact cttccagcaa cttgccgctg aggttgtcga tggcgcggtc aatgagctgg
     8581 ctgcgatcgt atgttgtaac tatctccaca ccttccggca gactactttt cagcgtttcc
     8641 agtttgtcct tcacggcggc gattacttct cgggcgtttt tgccggagcg caggatcacc
     8701 accccgcccg ccacttcgcc ttcgccgttc agttcggcaa tgccccggcg catctccggg
     8761 ccaatctgga ccttcgcaac atcacgcaga taaacgggca cgccattttc actggctttt
     8821 aaaacgatgt gattaaagtc gtcgagcgtt tgcagatagc cgctggctcg caccatatat
     8881 tccgcttccg ccagttcgat cgacgaacca cccgcttcct ggtttgaagc atccagcgcg
     8941 ctttttactt cggcgagact gatgccatat tgcgccaggc gctgaggatc gataaccacc
     9001 tgatactctt tcaccacacc gcctaccgac gccacttccg caacgtcagg aatggttttc
     9061 agctcatatt tgagaaacca gtcctgcaat gagcgtaaat cggcaagatc gtgcttaccg
     9121 ctgcgatcca ccagtgcata ttcatagatc cagccaacac ccgtggcatc tggccccagc
     9181 tcggcgctga ctcccgcagg cagcttaccc tgtacctggt tgaggtactc cagcacccgc
     9241 gagcgcgccc agtacggatc ggtgccatct tcgaaaatga catacacata agagtcgcca
     9301 aactgcgaga aaccgcgcac agtcttcgcg ccaggcaccg acaacatggt ggtggttagc
     9361 ggataagtca cctgattttc aacgatttgc ggtgcctgac cgggatagct ggttttaata
     9421 atcacctgca catcagagag atccggcagt gcatccactg gcgtgttaat gatggtccag
     9481 gtgccccaga tactcagaaa caacgcgccc atcagcacca gaaaacggtt cgccaccgag
     9541 cgacgaataa tccattcaat cattggttat tccctcaatg cgcatgggta gcactttcag
     9601 agcgcatccg ctccagtgcg ccagaaatat tggcttcgga atcaatcagg aacaggccgc
     9661 tggaaaccac cttttcacct tccgccagac cagagcgtaa tgcggtgacg ccttgcgatg
     9721 cctggaaaac ggcaacgcgt ttcggtacaa agcgcccgtc ggcatcaacg gtaatcaccc
     9781 gctgttcgct gccggtatca atcagcgctt gtgacggaat gagcagcatc ggttcgctgg
     9841 cggtgttgag ttgcaaccag gcgttcattc ccggctttag cgcctcgtcg gtgttgtcga
     9901 cttccaggcg cagctgcagc gtgcgggttg cggcatccac gccgggtagc agcgtccatt
     9961 tgcggatggt gagtgtttta tcaggtcgtg ccggaacggt gagcgtaaac tgcgaggcat
    10021 ctttcaccag ccaggcgatg gactccggga tcgcggcagt gatccacacc gggtccatac
    10081 cctgaatttt cgcgaccacg ttatctttgg cgatattcat tcccgcgcgc agatcaaacg
    10141 cggtgatcac gccatcaatg ggcgatttga gcgtaaagcg agtctggatt ttttgcgtgg
    10201 cgatcaggcg gcgaatatcc gcctccggca ttcccgccag tcgcagtcgc tcaagaatgc
    10261 cttcagtctg ggtcgccgta ccgccggttt cgcgcagcag taaatactca ctctgcgctt
    10321 ccacccagtc gggaatggtc aggtcgagaa gcggtgtgcc cttttgtact ttatcgccca
    10381 cggtaagcgg atacacctta tcgataaacc cggcagcgcg agcctgcaca atggcatact
    10441 gatactcgtt gtaactgaca ttcgccggga aactttgggc aaaagtcagc ggcccgcgcg
    10501 tgacggtcgc cgttttcacc cccagattct gcgtctgggt cgggtcaatg cgtacaccag
    10561 acgcagaact ctcttcatcg gcatatttcg gcaccagatc catatccata aacggcgatt
    10621 tacctggttt atcgaaccgc gtattgggat acatcgggtc gtaccagaat aagattttac
    10681 gttctgcggt cggcgttttt tctgtgggcg gttcagcctt tgcaaaccag gtaaaacctg
    10741 ccgcagaaat aataccgccc gcgatcatgc tgccgataat aagcgcgatt tttttcatct
    10801 cattaaacct gggttactgg ctgactttaa tatcctgtaa taaagaaagg ttgccctgct
    10861 ggacaaaatt aaacgccact ttgtcgccgg ttttaatttc actcattttc gtctgcgggg
    10921 tgatggtaaa gcgcatggtc atctccggcc agttcaccgc ggtaattgga tcgtggtgga
    10981 tggtgatttt tttgctttcc agatcaatgg tttttaccac accggtggcg ctaatgacct
    11041 gtggttgtgc ctcgctcatg gtttcatgat gatgttcgtt agcctgggca ttaaagccaa
    11101 taacggtaaa cagactaaac attgcgactt gcagtgcttt tttcatttat tctctcctgg
    11161 agttaaaaat tattgctgcc aaccgccccc taaggcggta tataaagaaa tttcgttaac
    11221 ctgacgggca taattcaggt caagtaaagt ttgtcgggtt gcaaataaag aacgctcggc
    11281 atccagcact tccagataac tgactgcgcc gtgctgatat aatgtccgcg cccgttgcaa
    11341 agtaatttgc agcgacgcca gataacgctg ctgggcgctg atttgatcgt tcaggctttg
    11401 acgtaatgcc agcgcatctg ccacttcttt aaaggcgttc tggattttct gttcataatt
    11461 caccaccgac tgctgctggc gaatttcggc gatatccaga ttggcctggt tgcgtccggc
    11521 attaaaaatg gggatctcaa ttttaggaat aaaattccac atcccgctgc tggcgttaaa
    11581 taatgacgac agatcgctgc tggcggttga tattccgctg gtcaggctga tagacgggaa
    11641 aaatgccgca cgcgcagcac caatattggc attagccgcc attaacgcgt gttcagcttc
    11701 cataatatca gggcgctgca ataagatttg cgacggcaag cccgccggta atttaacgct
    11761 ttgcaggctg tcgctgttta ctgtctgcgc ttgcggcagc ttgccgtagc ttcccaataa
    11821 cagttgcaat gcattattcg cctgcgccag ttccccctga cgtttagcga tgtcgctgcg
    11881 ggtactttca atcaccccgc gagcctgttc cagcgccaga acattactgc tacctgtcaa
    11941 cagttgtttt tcgacaaacg catatgactg ctgataatta cgcagcgttt cttcggctat
    12001 ttgcaattgc gcatacgcca gctgctgatt gaaatagctt tgcgcgacat tagaaaccag
    12061 cagaatatgc accgcgcgct gggcttcctc agtggctaaa taattttggc gctcggcttc
    12121 gctcatgttc tttaagcgac cgaaaaaatc gagatcaaag ctggcgttaa ggccagtcga
    12181 gaactcccgc gtcgaggctg aatcgccttt aagattgttg ctccagctgc cgctgccctc
    12241 gccattgagc tgtgggtagc ggtcggcatc ggtcagacga tattgcgccc gcgcttcctg
    12301 cactttcagc gtcgccatgc gcaaatcccg gttattcacc agcgcctcgc taatcagcgt
    12361 cttcacctga ttatcaacaa aaaaggtgcg ccagcccgcg ttctggtagt tatctgctgc
    12421 gttaaccagg ccgttctggt tgagtgagaa ctgctgcggc acgggcattg ccgggcgctg
    12481 ataatccggt gccagtgaac aaccggttag cgcaagggcc acacaaaatg gcagaagttt
    12541 acaaggagac ataggctcat aatttctggt gattttatgc cgccaacttt actcgccagg
    12601 ctctgatttt ccggtgacag gaaaatgaca aaattgtcat tttgccaata agcgattgcc
    12661 atctgatccc gctactctag aattgcccgg gcaacatgcg gaggaaatat gaaactgttg
    12721 attgtcgaag atgaaaagaa aaccggagaa tacttgacca aagggttaac cgaagccggt
    12781 tttgtggtcg atttggccga caacgggctg aatggctacc atctggcgat gaccggtgat
    12841 tatgatctga taatcctcga tattatgctg ccggacgtga acggctggga tatcgtgcgc
    12901 atgttgcgtt ccgccaataa ggggatgccg attctgttgc ttaccgcgct tggcaccatt
    12961 gaacatcgcg tcaaggggct ggagttgggg gcggatgact atctggtgaa gccgttcgct
    13021 tttgctgaac tgctggcgcg ggtgcgcact ctactgcggc gcggggcggc ggtgattatc
    13081 gaaagtcagt ttcaggttgc cgatctgatg gtcgatctcg tcagccgcaa agtcacccgc
    13141 agtggcacgc gcatcactct gaccagtaaa gaatttactc tgctggagtt tttccttcgc
    13201 catcagggcg aagtactgcc ccgctcactt attgcctcac aggtgtggga catgaatttc
    13261 gacagcgaca ctaacgccat tgatgtggcg gtgaagcggc tgcgtggcaa aatcgacaac
    13321 gactttgagc cgaagctgat tcaaaccgtg cgcggcgtgg gttacatgct agaggtgccg
    13381 gatggtcagt aagccatttc agcgcccgtt ttcgctggca acccgcctga ccttttttat
    13441 cagcctcgcc accatcgcgg cgtttttcgc ctttgcatgg atcatgatcc actcggtaaa
    13501 agtgcatttt gccgagcagg atattaatga tttaaaggag attagcgcca cccttgaacg
    13561 ggtacttaat caccctgacg aaacgcaagc ccgacgctta atgacgctgg aagatatcgt
    13621 cagtggttat tccaatgtgt tgatctccct ggcaaatagc cacggtaaaa cggtgtatca
    13681 ctcccccgga gcgccggata tccgcgagtt tacgcgtgac gccatacccg ataaagacgc
    13741 gcagggcggc gaggtgtatc tcctttccgg cccgacgatg atgatgccgg gccacggtca
    13801 cgggcatatg gaacacagca actggcggat gattagcttg ccggttggcc cactggtgga
    13861 cggcaaaccg atttatacgc tctacattgc gctttcgatc gattttcatc ttcattacat
    13921 aaatgatttg atgaataaac ttgtaatgac cgcatcgatc attagcatcc tgatcgtctt
    13981 tatcgtgctg ttggcggtac ataaaggtca cgcgccgatc cgcagcgtca gccgtcaaat
    14041 ccagaacatt acctcgaaag atctcgacgt tcgccttgac ccgcagaccg tgcccattga
    14101 gctggaacag ctggtactgt cgttcaacca tatgatcgag cgtattgagg atgtctttac
    14161 ccgccagtcc aatttctcag cggatatcgc ccacgaaatt cgcacaccta ttactaatct
    14221 cattacgcaa acggaaatcg ccctcagcca gtcgcgcagc cagaaggagc tggaagatgt
    14281 gcactactct aatctcgaag agctgacgcg aatggcgaaa atggtcagcg atatgctgtt
    14341 tctcgctcag gccgataaca accagctaat ccccgaaaag aaaatgctca acctggcgga
    14401 tgaagtcggc aaagtgttcg attttttcga ggcgttagca gaagatcgcg gcgtggagct
    14461 gcggtttgtt ggcgacgagt gtcaggttgc gggcgatccg ctgatgctgc gtcgggcgtt
    14521 aagcaacctg ctttctaatg ccctgcgtta tacgccaacc ggagagacaa ttgtcgtgcg
    14581 ctgccagacg gtcgatcacc tggtgcaagt taccgtcgaa aaccccggta cgcccattgc
    14641 gcccgaacac ttaccgcgat tgtttgaccg tttctaccgc attgatcctt cccgccagcg
    14701 aaaaggcgaa ggtagcggca ttggtctggc gatagtgaaa tcgattgttg tcgcgcataa
    14761 aggcactgtt gcagtaacgt cagatgcacg ggggacaagg tttgttatca tattacccgc
    14821 ttaatccttc agcaaacggc aacttttata accagtgtaa aaataacatg ccgcaatagt
    14881 aatacctatt aatgataaaa agctgtcaca attcataaaa aaccttaata tacgccaccc
    14941 taaacataac cagcgttaat gtaaggtttt tgtgtggact ggcttcttga tgtttttgct
    15001 acctggctct acggcttaaa agtaatcgcg ataacgttag cggtcattat gttcatcagc
    15061 gggctggacg atttttttat tgatgtcgtc tactgggtac gccgcattaa acgcaagttg
    15121 agtgtttatc gccgctaccc gcgaatgagt taccgcgaac tgtataaacc agatgaaaaa
    15181 ccgttagcga ttatggttcc ggcgtggaat gaaacgggcg tcatcggcaa tatggccgag
    15241 ctggcggcga ccacgctcga ctacgaaaac tatcatatct ttgttggcac ctaccccaac
    15301 gaccccgata ctcagcgtga tgttgacgaa gtgtgcgctc gcttcccgaa cgtgcataag
    15361 gtagtctgcg cgcgtcctgg ccccaccagc aaagccgact gtctgaacaa cgtgctggac
    15421 gccatcaccc aatttgaacg tagcgccaat ttcgcttttg ctggttttat tctgcatgac
    15481 gccgaagatg tgatttcacc gatggaattg cgtctgttca actatctggt cgagcgtaaa
    15541 gatctgattc agatcccagt gtatccgttc gaacgcgaat ggacgcactt caccagcatg
    15601 acctacattg atgagttttc agaactgcat ggcaaagatg ttccggtgcg tgaagccctc
    15661 gccggacagg tgcccagcgc aggcgtcggc acctgtttca gccgccgcgc cgtgaccgca
    15721 ctgttagctg acggtgacgg tattgctttc gacgtgcaga gtctgactga agattacgac
    15781 attggcttcc gcctgaaaga gaaaggtatg acggaaattt ttgtccgttt tccggtggtg
    15841 gacgaagcca aagaacgcga gcagcgtaaa tttttacagc atgcgcggac gtcaaacatg
    15901 atctgcgtgc gcgaatattt ccccgatacc ttttcgactg cggttcggca aaaatctcgc
    15961 tggatcatcg gcattgtttt ccagggcttt aaaacccaca aatggacctc cagcctgacg
    16021 ctgaactact ttctctggcg cgaccgcaaa ggggcaatca gtaactttgt cagcttcctc
    16081 gcgatgctgg tgatgataca gcttttgctg ttgctggcgt atgaaagttt gtggcccgat
    16141 gcctggcatt tcctttctat tttcagcggc agcgcatggt taatgactct gctgtggcta
    16201 aactttggct tgatgattaa ccgcatcgtg cagcgggtga ttttcgttac tggctactac
    16261 ggcctgacgc aggggctgct atctgtcctg cgtcttttct ggggcaacct gattaacttc
    16321 atggccaact ggcgcgcgtt aaaacaggta cttcaacacg gcgatccacg tcgcgtcgcg
    16381 tgggataaaa caacgcatga cttccccagc gtgactggcg atacccgctc gttgcgcccg
    16441 ttaggccaaa ttctgctgga aaatcaggtc atcactgaag aacaactcga tacagcactg
    16501 cgtaatcgcg tcgaaggtct acgcctgggc ggttcaatgc tgatgcaggg gctgattagc
    16561 gccgaacagc tggcacaggc gctggcagag caaaacggcg tggcgtggga gtcgatcgac
    16621 gcctggcaga tcccttcatc actgattgcc gaaatgccgg cttccgtggc gctgcattat
    16681 gcagtgctgc cgctgcgtct ggaaaatgat gagttaattg tcggcagtga agatggtatt
    16741 gacccggttt cgctggcagc cctgacgcgt aaagtcggac gcaaagtgcg ttacgtcatt
    16801 gttctgcgcg gacaaattgt cacggggtta cgtcactggt atgcacgccg acgcggtcac
    16861 gatccgcggg caatgttgta caatgcggtt cagcatcagt ggctcacgga acagcaggcc
    16921 gttgaaatct ggcggcaata tgtgccgcat cagttcctgt tcgccgaaat actgaccacg
    16981 ctcggtcata ttaatcgttc agcaattaac gtgttgttat tgcgccatga acgcagttct
    17041 ctgccgctcg gcaagttttt ggtcaccgaa ggcgttatca gccaggaaac gttggatcgc
    17101 gtcctgacaa ttcaacgcga attacaagtt tcgatgcaat cactattact caaagcaggt
    17161 ttaaacccag aacaggttgc gcaactggag tccgaaaatg aaggagaata accttaatcg
    17221 cgtcatcgga tggtctggtt tactgctgac gtctttattg agtaccagcg cactcgcaga
    17281 caatatcggc accagcgcag aagagctggg gctgagcgat tatcgccatt ttgttattta
    17341 tccccgtctc gataaggcgc tgaaggcaca gaaaaataac gacgaagcaa ccgccatccg
    17401 cgaatttgaa tatatacacc aacaggtgcc ggataatatt ccgctaactt tataccttgc
    17461 ggaagcctat cgccattttg gtcatgatga ccgggcgcgg ctgttgcttg aggatcaact
    17521 gaaacgtcac ccaggagatg cccgacttga gcgcagtctg gcggctattc cagttgaagt
    17581 gaaaagcgtt acgactgttg aagaactgct tgcccagcaa aaagcgtgcg atgctgcgcc
    17641 gaccctgcgt tgtcgcagtg aagtcgggca gaatgccctg cggctggcac aattacctgt
    17701 cgccagagcg caactgaacg atgcgacgtt tgctgcatcg ccggaaggaa aaacgctgcg
    17761 aaccgatcta ctgcaacggg caatctacct gaaacaatgg tcccaggcag atacgctata
    17821 caatgaagca cgccagcaga acacattaag tgcggcagaa cgccgtcagt ggtttgacgt
    17881 gcttcttgcc gggcagctgg acgatcggat cctggcgctg caatcacagg ggatcttcac
    17941 cgatcctcag tcatatatta cttacgcgac cgcgctggct tatcgtggcg aaaaagcacg
    18001 cctccagcat tatctcattg aaaatacgcc gctatttacc acggacgcac aagagaaaag
    18061 ttggctctat ctgttatcta aatacagcgg caaccccgtt caggcgttgg cgaattatac
    18121 ggtacagttt gccgacaacc gccagtatgt tgttggcgcg acgctaccgg tgctgttaaa
    18181 agaaggtcag tacgacgcag cgcaaaaact gctcgccacc ctccccgcca atgaaatgct
    18241 tgaggagcgt tataccgtta gcgtggcgac ccgtaacaag gctgaagctc tgcgtctggc
    18301 acgattgctg tatcagcaag aaccggcaaa tcttacccgc ctggatcaac tgacctggca
    18361 actgatgcag aacgagcagt cacgtgaagc tgccgattta ttgctgcaac gctatccttt
    18421 ccagggcgat gcgcgtgtca gccagacttt aatggcgcga ctggcgtctc tgctggaaag
    18481 tcatccttac ctggcaactc cggcgaaggt ggcgatttta tcgaaaccct taccgctggc
    18541 ggagcaacgt cagtggcaaa gtcagttgcc gggtattgca gataattgcc cggcaatagt
    18601 tcgcttgctg ggcgatatgt cgccttccta cgatgctgcc gcctggaacc gtctggcaaa
    18661 gtgttatcgg gacacgctac ccggtgtggc gttgtatgca tggcttcagg ccgaacaacg
    18721 acaaccgaac gcctggcaac atcgtgcggt agcctatcag gcgtatcagg ttgaggacta
    18781 cgccaccgca ctggcggcct ggcagaaaat cagtcttcac ggcatgagca atgaggatct
    18841 gcttgctgct gccaataccg cccaggcggc aggaaatggt gcggctcgtg atcgctggct
    18901 tcagcaggca gaacaacgtg ggctgggaaa caatgccctc tactggtggc tgcatgcgca
    18961 acgttacatt cctggtcagc cggaactcgc actgagcgat ctcacgcgct aaatcaatat
    19021 tgcgccttct gccaacgctt acgttgcgcg ggcgacaatt tatcgccaac gtcataatgt
    19081 cccggcggcg gtgagtgatt tgcgcgccgc tctggaactg gaaccgaata atagcaacac
    19141 ccaggcagcg ctcggttacg ccttgtggga tagcggtgat atcgcacagt cgcgggaaat
    19201 gctcgaacag gcgcataaag ggctaccgga cgatccggca cttatccgac aactggccta
    19261 cgtgaaccag cgtctggatg acatgcctgc gacgcagcac tacgcccgac tggtgattga
    19321 tgacattgat aatcaggcgc tgataacccc actgacccca gagcaaaatc agcaacgctt
    19381 caatttccgc cgtctgcatg aggaggtcgg tcgccactgg acgttcagtt tcgattcttc
    19441 tatcggcttg cgttccggcg caatgagtac cgctaacaat aatgtcggcg gcgcagcgcc
    19501 agggaaaagc tatcgtagct acgggcaact ggaagccgag taccgcatcg gacgcaatat
    19561 gctgctggaa ggcgacctgc tctcagttta tagccgcgtc tttgccgata ccggtgaaaa
    19621 cggggtgatg atgccggtga aaaatccgat gtccggcacc ggtctgcgct ggaagccgct
    19681 gcgcgatcag atctttttcc tcgccgtcga acagcagttg ccgctgaacg gacaaaatgg
    19741 cgcatccgat accatgctgc gcgccagcgc ctcattcttt aatggcggca aatacagcga
    19801 cgaatggcac ccgaacggtt caggctggtt tgcccaaaac ctgtacctcg atgcggcgca
    19861 atatatccgc caggatattc aggcgtggac ggcagattat cgagtcagct ggcatcagaa
    19921 ggtggcaaac gggcagacta ttgagcctta tgctcacgtt caggacaacg gttatcgtga
    19981 taaaggcact cagggcgcgc agcttggcgg tgtcggggtc cgctggaata tctggaccgg
    20041 cgagacgcac tacgacgcct ggccgcacaa agtcagtctc ggcatcgaat atcaacatac
    20101 ctttaaggcg attaatcaac gtaacggaga gcgcaacaac gcgtttctca ccattggagt
    20161 gcactggtaa atgcgtaagt tcattttcgt attgttgaca ctgcttttgg tcagcccttt
    20221 ttcctttgcg atgaaaggta ttatctggca accacaaaac cgtgatagtc aggttaccga
    20281 tacccagtgg caggggctga tgagtcagtt acgtttgcaa ggcttcgata ccctggtttt
    20341 gcaatggacc cgttacggcg atgcattcac ccagccagaa cagcgcgcgt tattgtttaa
    20401 gcgtgccgca gctgcgcaac aggctggcct gaagctgatt gtcgggctga acgccgatcc
    20461 ggaatttttt atacaccaga aacagtcctc cgcggcgctg gaaagctatc ttaatcgcct
    20521 gctggctgcc gatctccagc aagccagatt atggagcgcc gtgcctggcg taacgccgga
    20581 tggctggtac atcagcgcgg aaattgacga cctgaactgg cgcagcgaag ccgcccgtca
    20641 acctttgcta acatggttaa acaacgcgca gcggttgatt agcgatgttt cagcaaaacc
    20701 ggtttatatc agtagttttt tcgccggaaa catgtcgcca gatggctatc gccaactgct
    20761 ggaacaagtt aaagcaaccg gtgttaatgt ctgggtacag gatggcagcg gcgtggataa
    20821 actgaccgct gaacagcgtg aacgttatct acaggccagc gccgattgcc aaagttccgc
    20881 cccagcaagc ggcattgttt atgaactgtt tgtcgctggt aaaggtaaaa cgtttacagc
    20941 gaaaccgaaa ccggatgcag agatcgcctc gctgttagcg aaacgttcct cttgcggtaa
    21001 agacacgctc tatttctctc tgcgctattt gcccgtcgcg cagagtatcc ttgagtatta
    21061 atgattaaag ggagcaactt agcttgctcc tttccttttc actttcctaa tcaaaatcct
    21121 ggcaagatga attgtttagc agaatttctg ttttcatttt attcgtctga cgtgttccaa
    21181 ttatttagca aaactgcgac aacaaagtga tggtgtaatt tgtataaggg aacaaataaa
    21241 tggcgaggaa atggctcgcc ctgcaggatt cgaacctgta gcccacgact tagaaactcc
    21301 aggaacgaca ttttaaatca acaaattacc gcgccatctc tgcgttcaca cgttccacca
    21361 cctcaaaata tgtaaaacct tgcaagccat tgcgaggcct tatgtgtctc agttttgttc
    21421 ctctttttta tactaaaaaa cacagcaatt gaggataaac ctcatgctat tatcgctaat
    21481 atgcatctaa aggcatggca cttgaataga taaaaactcc acaaagaaaa aataattagc
    21541 ccataaaacc acaatgcaaa acacaaacag acaattaaac taaaaaactg acagtgtatt
    21601 gtgacaaaaa ttcaatgtta aacaaaatat aaacagtggg gcaatataca attgactcat
    21661 taagttagat ataaaaaata catactcaat cattaaaacg attgaatgga gaccttttat
    21721 gcgggcgaaa cttctgggaa tagtcctgac aacccctatt gcgatcagct cttttgcttc
    21781 taccgagact ttatcgttta ctcctgacaa cataaatgcg gacattagtc ttggaactct
    21841 gagcggaaaa acaaaagagc gtgtttatct agccgaagaa ggaggccgaa aagtcagtca
    21901 acttgactgg aaattcaata acgctgcaat tattaaaggg gcaattaatt gggatttgat
    21961 gccccagata tctatcgggg ctgctggctg gacaactctc ggtagccgag gtggcaatat
    22021 ggtcgatc
//
LOCUS       DAPHCV010000084        21836 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.084, whole genome shotgun sequence.
ACCESSION   DAPHCV010000084 DAPHCV010000000
VERSION     DAPHCV010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.084"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            243..1142
                     /gene="yegS"
                     /locus_tag="RH033_003223"
     CDS             243..1142
                     /gene="yegS"
                     /locus_tag="RH033_003223"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HDV2992371.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(1224..1997)
                     /locus_tag="RH033_003224"
     CDS             complement(1224..1997)
                     /locus_tag="RH033_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDV2992372.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(2103..3143)
                     /gene="gatD"
                     /locus_tag="RH033_003225"
     CDS             complement(2103..3143)
                     /gene="gatD"
                     /locus_tag="RH033_003225"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDV2992373.1"
                     /translation="MKSVVNDTDGVVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVNRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQGVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVDTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(3191..4546)
                     /locus_tag="RH033_003226"
     CDS             complement(3191..4546)
                     /locus_tag="RH033_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDV2992374.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFYKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDTIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQEKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
     gene            complement(4550..4834)
                     /gene="gatB"
                     /locus_tag="RH033_003227"
     CDS             complement(4550..4834)
                     /gene="gatB"
                     /locus_tag="RH033_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HDV2992375.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(4865..5317)
                     /gene="gatA"
                     /locus_tag="RH033_003228"
     CDS             complement(4865..5317)
                     /gene="gatA"
                     /locus_tag="RH033_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HDV2992376.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVYDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(5327..6589)
                     /gene="gatZ"
                     /locus_tag="RH033_003229"
                     /pseudo
     CDS             complement(5327..6589)
                     /gene="gatZ"
                     /locus_tag="RH033_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            complement(6618..7472)
                     /gene="gatY"
                     /locus_tag="RH033_003230"
     CDS             complement(6618..7472)
                     /gene="gatY"
                     /locus_tag="RH033_003230"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDV2992377.1"
                     /translation="MCVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(7781..8833)
                     /gene="fbaB"
                     /locus_tag="RH033_003231"
     CDS             complement(7781..8833)
                     /gene="fbaB"
                     /locus_tag="RH033_003231"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HDV2992378.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            9090..10367
                     /locus_tag="RH033_003232"
     CDS             9090..10367
                     /locus_tag="RH033_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2992379.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYPEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            10364..11368
                     /locus_tag="RH033_003233"
     CDS             10364..11368
                     /locus_tag="RH033_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HDV2992380.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            11365..12330
                     /locus_tag="RH033_003234"
     CDS             11365..12330
                     /locus_tag="RH033_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDV2992381.1"
                     /translation="MSGARLHTLLPELTSRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGNQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(12304..13050)
                     /locus_tag="RH033_003235"
     CDS             complement(12304..13050)
                     /locus_tag="RH033_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDV2992382.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(13102..13920)
                     /locus_tag="RH033_003236"
     CDS             complement(13102..13920)
                     /locus_tag="RH033_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HDV2992383.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAIQIKD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTEEELQAFVDGIKETP"
     gene            complement(13985..14785)
                     /gene="thiD"
                     /locus_tag="RH033_003237"
     CDS             complement(13985..14785)
                     /gene="thiD"
                     /locus_tag="RH033_003237"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDV2992384.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(14782..15570)
                     /gene="thiM"
                     /locus_tag="RH033_003238"
     CDS             complement(14782..15570)
                     /gene="thiM"
                     /locus_tag="RH033_003238"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDV2992385.1"
                     /translation="MQVDLLSSAQSAHTLHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRTQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDML
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(15605..15701)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15793..16065)
                     /gene="rcnR"
                     /locus_tag="RH033_003239"
     CDS             complement(15793..16065)
                     /gene="rcnR"
                     /locus_tag="RH033_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HDV2992386.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            16186..17010
                     /gene="rcnA"
                     /locus_tag="RH033_003240"
     CDS             16186..17010
                     /gene="rcnA"
                     /locus_tag="RH033_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HDV2992387.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHGHDYEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            17229..17567
                     /gene="rcnB"
                     /locus_tag="RH033_003241"
     CDS             17229..17567
                     /gene="rcnB"
                     /locus_tag="RH033_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HDV2992388.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(17649..18683)
                     /locus_tag="RH033_003242"
     CDS             complement(17649..18683)
                     /locus_tag="RH033_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HDV2992389.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTNGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNSSPVTLLNITNSSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(18699..21179)
                     /locus_tag="RH033_003243"
     CDS             complement(18699..21179)
                     /locus_tag="RH033_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV2992390.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYL
                     SQYYSDYKASGNNKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDLAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGAGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     STPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVIYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQSLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(21195..21836)
                     /locus_tag="RH033_003244"
     CDS             complement(21195..21836)
                     /locus_tag="RH033_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDV2992391.1"
                     /translation="MVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQGNRSSLLQAWID
                     DGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKESIFYLNVLDIPP
                     NSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNGLVIKNDSANWV
                     TISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGNYISDKI"
BASE COUNT         5485 a         5576 c         5065 g         5710 t
ORIGIN      
        1 atttggaata gtatatttaa gatgccactg tttggttgaa tattaggcat atgctctttt
       61 ttgaaattta tcggtggcag ccgttagtat tcgctgtccc cattgcaagc tcctggtggt
      121 aaccactgaa tcctccatac ttgaactgac tttttatcct ccgactttca tcctgttctg
      181 actccacctt ttgttttctg ctctacacta tctacagacc aatcataaag gcacatacga
      241 tcatggcaga atttcccgcc agcttactga ttcttaatgg caaaagtact gacaatctac
      301 ccttgcgcga agcaattatg ctgttgcgtg aggaaggaat gacgatccat gtgcgggtca
      361 cctgggagaa aggcgatgcc gcacgatatg tagaggaggc ccggaagttg ggcgtcgcaa
      421 cggtgattgc cggtggtggc gatggcacca ttaatgaagt ttctacggcg ttgattcagt
      481 gtgaggggga tgacataccc gcgctgggaa ttttgccatt aggaaccgcc aatgattttg
      541 ccaccagtgt agggattcct gaggcactgg ataaggcgct gaaactggca attgccggta
      601 acgccattgc gatagatatg gcgcaggtca acaaacaaac ctgttttatt aatatggcga
      661 caggcggatt tgggacgcgt attaccacag aaacgccgga aaaattaaaa gccgcgctgg
      721 gtggcgtctc ttacatcatt catggcttaa tgcgcatgga tactctgcaa ccggaccgtt
      781 gtgaaatccg cggtgaaaac tttcactggc aaggtgacgc cctggtcatt ggtattggta
      841 acgggcgtca ggccggtggc ggtcagcaat tgtgtccgaa cgcgttaatt aacgatggct
      901 tgctgcaact gcgcattttt accggcgatg aaattcttcc ggctctcgta tcaaccttaa
      961 aatctgacga agataacccg aatattatcg aaggcgcttc gtcgtggttt gatatacaag
     1021 ccccacacga aatcactttt aatcttgatg gcgaaccgtt gagcggacaa aactttcata
     1081 ttgaaatact tccggcggcg ttgcgttgtc gattaccacc agattgtcca ttattgcgtt
     1141 aatcaaacta ttctctcttg aaccagggag aatccgaaat gccggatgcg gtgtaaatac
     1201 cttatccggc atacagaaca atactacacc gtaattaatt ttacccgtgt cttctgcaat
     1261 gccagtttat ccacctctga tacagcatca tcggtaataa taatgtcaat tttttcgatt
     1321 ggcagtacct gattaaaacc gcgacggtta aatttcgatg aatcgagaac ggcaatcact
     1381 ttattagcgg cggtgaccat cgcaccgcta atcgaataac cttcattaaa ggtcgtaata
     1441 ccattaaccg catcaatacc atcagcaccg acaaacatta aatcggcatt aatatcctgc
     1501 agggaacgtt cagcaataga tccatgcata gatcgcgttt tatggcggac cgtgccaccg
     1561 cagacgacca gagtaatgtc tttattttcg gaaagggcaa acgcagccgg gagactgttg
     1621 gtaatcaccg tgatattttt ggcggtcatt aatccttcgg cgataagcat tgtggtactt
     1681 ccgctgtcga gaataacggt catcccttca tggatcattt tgactgccgc ctgcgctatt
     1741 ctgtttttgg gcgcgctggc gagctgaaat cgctctttaa acccgacttc ctgggtctcg
     1801 gtttcactat taccggacat tattttcgcc gcaccgccat gaaagcgcgt aaccacgcct
     1861 ttttgttcga gaaagcgcaa atcggcacgg attgtcgctt ccgaggcagc aaatactccc
     1921 gccagatcct gaacaagcac ggttccctgt tcattcacta attggatgat cttattcctt
     1981 cgctcgaatg aattcatcgt catttaccca tattcaattg tggctagtgt aaacgaaggg
     2041 ggaccattgg tgaatgatct gttttgcaca ataccgggcc agcatcacgc tggcccgcgg
     2101 tttcagggaa tgagcaacac tttgcccggc atggcattac gagcgatgtc acgcaccgcc
     2161 tgggcgaagc tttcaaagct tccacggtga gcgattaatg gctccaggct taacttacgt
     2221 tctgtcagca accggctcgc cgtttcccac tcctgccccg gccaagggct ggagtagttc
     2281 atccaactgc cgataaccgt cagctcttta cgcaatattt tgccaaacgt tgccgatgtt
     2341 aaatgcagat cctgatgcaa cgtgtccacc agcgccagtt gggcatgagg accggcaatc
     2401 tctaccgcca gttcgacagt ttgcggtacg ccagccgtct cgaggataag ctgattaaag
     2461 cgcagctcgc gtaaaacgcc ctgcatttgc ggcgcgctca tttcgctact gttaaatgtt
     2521 tgcatcgcac cgaaagattt tgccagtgcc agtttttctg agctaatgtc gatagccgtc
     2581 acactctttg ctcccagcgc gacagcgcac tgaatagcca gcaggccaat ggttccggca
     2641 ccaataataa taacgttttt attctcacaa ccttgcgcta aatgaaaagc atgcagacca
     2701 acggtaatcg gctcaataaa agccccatcc tcaataggca tatccgtggg tagagcaaag
     2761 acattttttc ggttaacgac aatatattcg gcaaatccac catcacgccg cgagccaata
     2821 aaatcatatt ttgcgcactg ggaataaaac cctttcaaac actctggaca agtaaaacag
     2881 ggtaataacg gcacacaggc aaccgcatcg ccagggtgta aatcatcaac accggatcct
     2941 accgcatcaa tatagccgct aaattcatgg cctaacgtta ttggataata atgtgcacca
     3001 tttttaaata tcctgggtaa atcggaacca cataagcccg agctggcaat ttttacccgc
     3061 acctcatcct gatgtttaat ttcaggaatg acgctttttg caacgcgcac gacaccatca
     3121 gtatcattca ctactgattt cataaaaact cctgattgtt aagggggata acctccccct
     3181 caggtaaaaa ttattctgcg agaacggctt tctcttgttt agtaaaacca cgcgctctac
     3241 gccaggtcat gaaaataccg gtcagataaa ttgcgccgat aataatgaaa ccgggaatat
     3301 tttgcgggga gaaaacctga atcagtaacc aggtaatggg agaaccgccc tgatccattg
     3361 aagccaccat acccccggct tttaacgccc cagcattagc cgccagttgg gtgtgcaaac
     3421 caatagtttg cgtcgcgatc cacagggtga tgctcataat gatgacaccc gagattaagg
     3481 tgcggaacag atttccacga tgcaccgcga ccgccatcgc cacgaagaag ccgatagtgg
     3541 caagatcgcc aaacggcagt acctgattac ccggaacaca aacagcaatt aaaatggtga
     3601 gcggaataaa aatcaggctt gccgatacca ccgccgtatg ccccagcagt aatgcgggat
     3661 ccaggccaat caggaactcc tgaccgccga acttctcctg taaacgacta cgggcctgct
     3721 tagcgatggg cgttaaacca tccatgatgg gtttaatcac ccgtggcatc agcagcatca
     3781 ccgccgccgt ttttaccgcc agctgcaata cacctttgac atcgtaaccc gcgaggatgc
     3841 cgataatcag ccccatcaca aaacccacgg tgacaggctc gccaaacgga ccaaatttac
     3901 gctgaatatc gtcggcgcta aatttaattc ggttaacgcc tgggattttc tcgatgatag
     3961 tatcgaccag caccgcaatc ggccccatat acgccgacgt accgtgcgga atagcaatgc
     4021 cttccagctc aaagaaattt cgggtatcgc gggcaaacca gtcgccgagc ttataaacaa
     4081 acgccgcgtg aattaccaca cccgccatcc ctatcatcca tgaaccggtt gccagatgca
     4141 gcaacgcgcc ggtgaaggtc atatgccaga tattccagat atcaacattt accacccgcg
     4201 tcatacgggt cagtaacatc gccacgttaa ccagaatcgc aatcggaatc gccaccagcg
     4261 caatttgcga cgcccaggtc attggtgaag agcccggcca gccgacatcg accacatgca
     4321 gattcaggtc gaaattttcc gccatcgctt tcgccgccgg accaatggaa tccagcatta
     4381 agccaatcac aaggccaatg ccaacaaagc caatcccgat atgcagaccc gctttaaagc
     4441 aatcgcctgc cttcatgcct aatattttat aaaaaataat gatgacaatc ggcagcatca
     4501 ccgtagggcc gaggtcgaga atataacgca tgacttctga aaacataggt cacccctgta
     4561 agatagtcag aattttattt tgtaatgctt cgataccgac accagaaaca aaaggcatgc
     4621 cgtgaactaa cggaatatcg ccaaaactac gatccacttt ggcagtggtg catatcaaat
     4681 gcacaccatc catataggtt tctatttcat taacccgaca ctggattaat tcaacaggaa
     4741 tattatgact ctgacacaac tctttaattt cttccgccgc catcgtagag gtcgcaaccg
     4801 cgcctccgca agcgacaata atcttgcgtt tcataaatag tcctttttat tgttacagag
     4861 ggatttattc gtctgaatct aaaacatact ttgtgaagta ttcctttaac tgggtttcag
     4921 gaagagtgat tagtgtctcg acgatatcgg gctgttgtaa cttgccaaat aaacagcgta
     4981 aaagtttcaa ttgctgctgc ggattttcca caatcaacgc aataaccaac gataccgcca
     5041 cgtcgttatc atcatccgct tgctgaaaat gaactttatt tgttggcctt aacagataaa
     5101 tggctgacga cttagcatga atcgcctcac aatgcggtat agcaatggcg tgctgctcaa
     5161 gcattatccc ggtagggaat tctgcttctc tggcaattaa tgcctgtggc caggtatcat
     5221 aaaccacacc tttcgcgagc atctcattac cgatatgggt taaaacttcg ctacgatcga
     5281 caaaagaaat tccgctacga acaaacaggt tagtcatata ccgtccttat tccgcacagc
     5341 cgtagcgata ggcgcgcaaa acatcataaa ttttatccat aatcagctga tgcggtattg
     5401 ctgataattc cccggactga atgcgttcaa attgtttggg aagatactga ctaatcatgc
     5461 ccagtgggat gtccacgcct tcaagattca ccatcatcgt ttcgacgcta tttttaatcc
     5521 gactatgcgg ccagtaataa cgaatacgat ccgacaggct gtaacgaata tccagtaatg
     5581 aatcgttaaa acccgtacga taatattttt tccagtattg cggttcgtcg agcatcactt
     5641 cttcaattac cgccaggcaa ccgctgcgat tttcaggggc gataagttcc tgctcaattt
     5701 gtgccagtgc aaatatcgcc tcgcgtaaag caaaggttaa tgcgggaccg actttcaata
     5761 ttgcaaagtg atcgcggact aattcccaat aagccgtccg ggtctggtaa tcggtagaat
     5821 gtgcttcata aaccattcgg gtgctttcta tccattgcgc cagcggctgc gcttcctgcg
     5881 gctgataatg gataatattg ctgtgatcaa attccacgcc cggctgcacc acgatggcaa
     5941 tcacgcgtgt taacgcctct gtcagcccac gggcaataaa ggccttttga tgcgtacgta
     6001 aagtattggc ggcatcttca acatgggtga tgtgtactga ctgaatggcg ctggcctcac
     6061 cgcccggaac cggaacttcg gtgccaatga catagctcag ttgctcacgc tggcaatctg
     6121 tcgccacact ttccgcagca aagcaaagca cagcagctcg ttccgcaacc gtttctggtg
     6181 ctaacggtat gggatccccc gcgcaggaca ttgacgcatc aagatgaatt ttactgaaac
     6241 cggcacgaac atattccttt accagctcga cggatttttc catcgccaca tccgcatttt
     6301 cttgctgcca gcagtttggc cccagatgat cgccgccgag aataatgcgt tcgcgtgcaa
     6361 acccaacttt atcggcaatc gtaaaaacaa attcgcgaaa gtctgccggt gtcattccgg
     6421 tataaccgcc aaattgattg acctagtttg acgttgcttc aatcagcact ttgcgcgtgc
     6481 tgttgcgatc aaatgccagc gccgcttcga taaccaacgg atgggcagaa cagactgaac
     6541 atatgccgat atgttcacca gctttatgcc gggcaattaa cgttttcatg ttttttcctt
     6601 attaaatggc aggtgcgtta tgccctgccc tcgcagccac aatcggcaat cactttgctc
     6661 accacatcgc gcattgcgga tttagccgac tgcaaataat cccggggatc ggtcgcttca
     6721 gggtgctcgg tcaggtaatt ttttaacgcc tgcgagaagg catttttcag ctccgttgca
     6781 acgttgattt tgcatatccc cagtttgatg gtttgctgaa tatccttagt cgataaccct
     6841 gacgcgccat gcagcaccag cggtaagttc acccactggc gaatgttctc cagtcgagaa
     6901 aaatcaagcg ccggtgcgct ggcatacatc ccatgagccg tgccgatggc gaccgccagg
     6961 gaatcaattc cggttgcctc ggcaaattca cgcgcctgag cggggttggt ataaaaagca
     7021 tcggcttcat tgacttgcac atcatcttcc tggccgccaa gttgccccag ctccgcttcg
     7081 acgctgacat caaagcgatg gcaaaaatcc accacctctt tgacccgtga aatattttgc
     7141 gcaaaaggca aatgcgaggc gtcaatcatg actgagcgca cgccagaacg aaccttctga
     7201 gcgatatcgt caaatttcgt gtgatggtcg agatgaattg ccagtggatg gtgatattgc
     7261 ttcgccatcg cgctgaccag cgccaacaga ttttctgtac cagcatgagt aaatgtgcca
     7321 ggcgttccgg cgatgatgac cggcgcatgc aggttggcag cggtttccac caccacttgc
     7381 atcgtttcga gattgtgaat attgaatgcc ggaaccgcat aaccgccgcg ctgtgcgttg
     7441 ttcagcatct gctttgtcga taccacgcac attttcatat cctgtcgttt gctttcgatt
     7501 tcaaaatata atgaaatgat ttgtttttaa atgtcgagat aacgatcaca aaaacgacaa
     7561 tgtgaaaatt gttcgaggag taaaaggcaa aaaaaacggc ctcccgatag ggaagccgta
     7621 gcaaagtgcg cgtgttttta tgccggatgc ggtgtaaaca tacgtggcag tgctatgtag
     7681 gcatgataag acgcttccgc gtcgcatcag gcataggttg ccggatgcag tgtaaacgcc
     7741 ttatccgtcc tacggggtgg tgctgttcgt atgaggcgta tcaggcaata gtaattttgc
     7801 tatcgagata aacgtcctgc acggcattaa tcagtttcac gccgtcagcc atcgatttct
     7861 tgaacgcctt acgtccaaga atcagcccca ttccgccagc gcgtttgttg ataaccgcag
     7921 tacgcactgc atcgctgagg tcagtttcac cgcccgcagc accgccggag tttatcaacc
     7981 cagcccgacc catatagcag ttagctaact gataacgcac cagatcaatc gggttttcgc
     8041 tggtcaattt gctgtaaaca cgatcgtcgg tgtaaccgta attaattgct ttatagccgc
     8101 cgttattttc cgccattttt tgtttgacga tatctgcacc gatggttgcc gccagatggt
     8161 ttgcctgacc ggtcaggtcg gcggaaacat ggtaatcaac gccatctttc ttaaaggcgg
     8221 agttacgcaa ataggcccac agcactgtca ccatacccag ctcgtgcgca cgttcaaaag
     8281 ccgcagaaat ttcttcaatc tggcgacgtg actcttccga gccaaaatag atagtcgcac
     8341 caaccgcaac cgcgcccatg ttgaacgcct gctccacgct ggcatacagc gtttgatcgt
     8401 aggtattcgg gtaacttagc gtctcgttgt gattaagttt gacgaggaat ggaatgcgat
     8461 gcgcataacg ccgcgatacc gacgccagca cgccgtaagt tgacgccaca cagttacagc
     8521 ccgcttcgat cgccagttca acaatgtttt tcgggtcaaa gtagagcggg ttagcagcaa
     8581 atgaagctcc ggcagagtgc tcaacgccct ggtcaaccgg cagaatagaa agatatcctg
     8641 tgccagccag acgcccggtg ttgtacaacg tctgcatatt acgtaacacc gctggcgggc
     8701 gattattgtc aatcattacg cggtctacgt agtcatgtcc ggggagataa agctggtcag
     8761 aaggaattgt catacaacgg tgctgtaaaa ggttgtcggc gtctttgcca agcaactgcg
     8821 caatatctgt cattactatg ctcccgtaaa ttccgattgg atatcggcta tggattgtcc
     8881 tggcccgcct tttgcgggca atcataatcc tggtcgttac gggcgagttt ttccatcttt
     8941 tggatgtttt ttcagcgttt tctgttggct cgattcatca gaaaaaatgt tagcgcggtc
     9001 aagttttcac cgcaaaggta tttaaaaggt attattaagt ggtattgtca tcgcgtacct
     9061 tacattacct gtcatgaagg aattaaaaga tgaaaacaac agcaaagctg tcgttcatga
     9121 tgtttgttga atggtttatc tggggcgcgt ggtttgtgcc attgtggttg tggttaagta
     9181 aaagcggttt tagtgccgga gaaattggct ggtcgtatgc ctgcacagcc attgcggcga
     9241 tcctgtcgcc aattctggtt ggctccatca ctgaccgctt tttctcggcg caaaaagtgc
     9301 tggcggtatt gatgttcgca ggcgcgctgc tgatgtattt cgctgcgcaa cagaccactt
     9361 ttgccgggtt cttcccgtta ctgctggcct actcgctaac ctatatgccg accattgcgc
     9421 tgactaacag tatcgctttt gccaacgtgc cggatgtgga gcgtgatttc ccgcgcattc
     9481 gtgtgatggg cactatcggc tggattgcct ctggtctggc atgtggtttc ttgccgcaaa
     9541 tgctggggta tgccgatatc tcaccgacta acatcccgct gctgattacc gccggaagtt
     9601 ctgctctgct cggtgtgttt gcgtttttcc tgcccgacac gccgccaaaa agcaccggca
     9661 aaatggacat taaagtcatg ctcggcctgg atgcgctgat cctgctgcgc gataaaaact
     9721 tcctcgtctt tttcttctgt tcattcctgt ttgcgatgcc actggcgttc tattacatct
     9781 ttgccaacgg ttatctgacc gaagttggca tgaaaaacgc caccggctgg atgacgctcg
     9841 gccagttctc tgaaatcttc tttatgctgg cattgccgtt tttcactaaa cgctttggta
     9901 tcaaaaaggt attattgctt ggtctggtca ccgctgcgat ccgctatggc ttctttattt
     9961 acggtagtgc ggatgaatat ttcacttacg cgttactgtt cctcggtatt ttgcttcacg
    10021 gcgtaagtta cgatttttac tacgttaccg cttacatcta tgtcgataaa aaagcccccg
    10081 tgcatatgcg taccgccgcg caggggctga tcacgctctg ctgccagggc ttcggcagtt
    10141 tgctcggcta tcgtcttggc ggtgtgatga tggaaaagat gttcgcttat ccggaaccgg
    10201 taaacgggct gactttcaac tggtccggga tgtggacttt cggcgcggtg atgattgcca
    10261 ttatcgccgt gctgttcatg atttttttcc gcgaatccga caacgaaatt acggctatca
    10321 aggtcgatga tcgcgatatt gcgttgacac aaggggaagt taaatgaaaa cagaacgtat
    10381 tctcggtgct ctttatgggc aggcgttagg ggatgcgatg gggatgccct ccgagctttg
    10441 gccacgcagc cgcgttaaag cacactttgg ctggattgac cgttttctgc ctggaccaaa
    10501 ggagaataac gcggcctgtt attttaaccg cgccgaattc accgacgata cctcgatggc
    10561 gctgtgtctg gcggatgcgt tactggaacg tgaaggcaag atcgatccgg atctgattgg
    10621 gcgtaatatt ctcgactggg cgctgcgttt cgacgccttt aacaaaaacg tactaggtcc
    10681 gacctcgaag attgcgctta acgccattcg cgacggtaaa cccgttgctg aactggaaaa
    10741 caacggcgtg accaacggcg cagcgatgcg cgtctcgcca ttaggttgtt tgcttccggc
    10801 gcgtgatgtt gattccttta ttgatgatgt ggcgctggcg tcgagcccga cccataaatc
    10861 cgatctggca gttgcaggtg cggtagtcat cgcatgggcg atttctcgtg ccattgacgg
    10921 agaaagctgg tcagcgattg ttgattccct gccttcaatt gcgcgacatg cacagcaaaa
    10981 acgcatcact accttcagcg cctcactggc agcacgtctg gagattgcgc tgaaaattgt
    11041 gcgcaatgcc gacggcaccg aatccgccag cgaacagctt taccaggtcg ttggcgcagg
    11101 taccagcact attgagtccg ttccgtgcgc cattgcgctg gttgaactgg cacaaaccga
    11161 cccgaatcgc tgcgccgtcc tgtgcgctaa ccttggcggc gacacagaca ccatcggcgc
    11221 tatggcgacg gcgatttgcg gcgcgttgca tggcgttaac gctatcgatc ctgcattaaa
    11281 ggcggaactg gatgcggtaa atcagcttga tttcaaccgc tatgccacag cgctggcgaa
    11341 atatcgtcaa caacgggagg cggtatgagc ggcgctcgat tacacacgct gctgcctgaa
    11401 ttaacctcgc gtcagccggt gatggtcgtc ggcgcggcgg tcattgatgt gatcgccgac
    11461 gcttatgccc tcccctggcg tgggtgcgat atcgaactga agcagcagag cgttaacgtt
    11521 ggcggctgcg cactgaatat tgccgtggcg ttaaagcgcc tcggcatcga agcgggtaat
    11581 gccttgccgc tcggtcaggg cgtgtgggcg gagattattc gcaaccggat ggcaaaagag
    11641 ggcttaatca gcctgatcga taacgccgaa ggtgataacg gctggtgtct ggcactggtt
    11701 gagccggatg gcgaacgcac ttttatgtca ttcagcggtg tggaaaatca atggaatcgc
    11761 caatggttgg cacgattaac cgttgcgcct ggcaacctgc tctatttttc cggttatcaa
    11821 ctggcctcgc cctgcggcga attgttagtg gaatggctgg aaaagctgca agacgtgacg
    11881 ccgtttatcg attttggccc acgtatcggc gatatcccgg acgcattact ggcgcggatc
    11941 atggcctgtc gacctttagt atcactcaat cgtcaagagg ctgagattgc cgccgaacgt
    12001 tttgctttat ccgcagagat aacaacactt ggcaatcaat ggcaggagaa atttgccgcg
    12061 ccgttgatcg ttcgtctcga taaagaaggc gcatggtatt tcagcaacga cgcttctggc
    12121 tgcattccgg catttccaac gcaagttgtc gacaccattg gggcgggcga cagtcatgcc
    12181 ggtggcgtac ttgccgggct ggcctctggt ctgccactgg cggatgccgt attactgggc
    12241 aatgcagtgg cgtcgtgggt tgtcgggcat cggggcggtg attgtgcgcc aacgcgcgag
    12301 gaactactcc tcgcacacaa aaacgtatag atcgctgcga cagtggctaa tgctgtactc
    12361 aataggccgt tgctgttggt caagcgccac ttgcttgatc accagcaccg gtattttgct
    12421 atctaactga atatgtgact gaaactcggc atccggcatc cgggcgctaa cgcgggaacg
    12481 cgtacgttgt gggtaaatgt gctggctgcg gaaatagtca taaagtgaaa tcccgatggc
    12541 atcaacatca tgaattaaat gcgccggaac ccacgattcc tcaatcgata ccgcttcttc
    12601 atcgacatag cgaatacgct taagcaaaaa gacatcactt cctgcttcga ccgccagctg
    12661 ctgcgcgact tcttcgggac atttcacgac acgcttgtta acccatagcg tatcgggctt
    12721 tttcccgcgc aataccacct gctgagaaaa acctcgcgct tctttcaacg agtattcgaa
    12781 gatgttgttg atctgcgtgc cgtaaccgcg cgaacgcgtc accacaccct cttcttccag
    12841 cgcctgcatc gccttgcgca ccgtaatgcg cgaaacaccg gttaactgac ttaaatcacg
    12901 ctcaccgggc aaaatattgc catgctccag caccccgctg cgcacggcat tttttaccgt
    12961 ttcggcaaac ttaatataaa gcggcgtgtt atccgccgct aaaatacgct cgttcaactg
    13021 agcgataagc tgggtatgcg cttgttccat ttgtcttttt cctgccgggg agtctggtgg
    13081 gagtatatca ctgcttctta tttaaggcgt ttctttaatc ccatcaacga atgcctgcaa
    13141 ctcctcctct gtgccattaa acacattaaa atccaccgga ccattaatgc catctacctg
    13201 cccacggtcg gaatgctgcc agaaacgcca ggccatgccg tcattgtccg gacgacgttg
    13261 atagtagtga gccacccacc acggatattc attgaaatag cccgccagat tggtgtgata
    13321 aaaaacggct cctgagtaaa taatcggctt ttttcccgta cttttttcga ccatttttag
    13381 ccactgactt acccgcttgc gtaattcttt tgccgataat tttccccgtt cttctacgtc
    13441 cagcacggca gggaaatcgc cttgtgagaa atccaccgtt tgcagaaata atcgtgcctg
    13501 aaccggcgct gctaccgacg gggagaaata atgatacgcc ccgcgcagca ggccattttc
    13561 gcggcttagt tgccagttac gcgaaaaata ggggtccacc agcttttcgc cttccgtcgc
    13621 cttaataaaa gcaaactgta agcggatacc gttgtcgcgc atttttgcca cccgctgcca
    13681 gtcgatccgc tcctgccagc gagaaacatc tattccgtga atggtgtaac tggcaggaat
    13741 gcgaatagcg aaagatttaa ccgggcgata accgtagaaa tggatgtagt ctttaatctg
    13801 aattgctgtc gaatagaaaa aattcaccgt ctgacgcgga taaattgcaa cgatggaaat
    13861 tagcccaaga gcgcataata gagaggtgag tttttttcga ctggtaattc ttagctgcat
    13921 tggtttcatc cctgaatgtc agtgcaaaag actgacaata accaaagcag ctatagtacg
    13981 gtgctcacca ccaggcgtgg aagtggtgaa ccggaccaat accgtgacca acttccagcg
    14041 tgtcggcctg ggctaacgcc gatgaaagcc agctttttgc ctcctgtacg gtgtcagccc
    14101 agtttgtatg gcgcgggcgt agtgcagcca acgccgcaga gagtgtacaa ccagtgccgt
    14161 gggtgttttt ggtcataatg cgcggtgcgg taaaccgttg ttcaccctcg cgggtaaaca
    14221 gccagtccgg gctttgctca tcatccagat gaccaccttt cattagcact gcgccacagc
    14281 ccatcgccaa cagcgatcgc ccttgttcca gcatttcctg ttcggtgcgc gcgtgtggcg
    14341 cgtcgagcaa ggcggcagct tcgggcaagt ttggcgttat taatgaaacc tgtggcaata
    14401 atcgactgcg cagcgtagca accgccgaag gtgaaagcag cgggtcgccg ctttttgcca
    14461 gcataacggt gtcgagtacc acgttttgga tctgataacg ttgcaaccgt tctgccaccg
    14521 cttcaacaat atcggtttcc gccaacatac cgattttagt ggtatcgatt cgcacatcgc
    14581 tgaacaccga atcgagctgg gcggcgacaa aatcaggctc aatgcgatac accgactgta
    14641 cgccacgggt attttgcgcc accagtgcag taataactga gcaaccataa gcgccaagtg
    14701 ccgagaaggt tttaagatcg gcctgaatcc ccgcaccacc actcggatca gtaccggcaa
    14761 tcgtcagagc gttaattcgt ttcatgcctg cacctcctgc gtcaattgcc agagcgcatc
    14821 aaggaaatgt ggaacaaaac tgcctggccc ctcgcttctg gcgactgcgc gttctccggc
    14881 ttgtttcatc cagtgacagg cagatgcgac attttccagc atatcgcctg gtaacgcaca
    14941 acaggcagcg acaaccgccg ataatgcaca gccagttcct accactttgg tcattaacgg
    15001 atcgccaccg tgaatgccaa tgatacgatg tccatcggta acataatcca cctcgccagt
    15061 gaccacgacg attgcgccag tttcccgtgc cagtgtttgt gcagcgggta tcgcgttagc
    15121 tgcggcgtca gtggtatcca ctccccgccc accattagca atgccagcta atgccatgat
    15181 ttccgaagca ttaccacgta tcgctgccgg tttaaaagat aaaagttcat gacaaaaatg
    15241 gcggcgataa tcgagcgcac ccaccgctac tggatcaagc gtccagggtg tttgagagct
    15301 ttttgcttgc tcaacggcag cacgcatcgc ctgtgtgcgt ggctgcgtca gtgtgccaac
    15361 gttaatcaac aaggcactgg cgatagccgc aaactgactg gcctcttcgg tttcgataac
    15421 catcgctggc gatgcaccga gcgccagcaa ggtattggca gtaaaggttt gcaccacatc
    15481 gttggtcatg cagtgcacaa gaggggaatg ttggtgaaaa aggtgtaacg tgtgcgcaga
    15541 ttgcgctgaa ctcagcaggt cgacttgcat agtttgctcc tgccataacg tgaagaagca
    15601 atgacctggt ggtccgtgac ttccctacgc tggcattatc cagatcaggt gatacgggta
    15661 tttctcagcc ttcacgcaga agggcacccc gagtcgtttg gttgcgatga taaatcgcag
    15721 aggaggatgg taatgtccag cgcacgcgtt gtaaacgagg aaaaacggca gttttacaat
    15781 cgcgttaata aattatttga tatatgaatc cagcaccttc agaacgacat ccagatcttc
    15841 ttcacgtttt agctcatccc cctggtgaac gatatgttcc gttagatgac ctttaatcac
    15901 ttcccgcatc agaccgttta ccgcgccacg gatagcagca atctgttgta aaactgcagc
    15961 gcattcgtgc ggctcgtcga gcattttctt gagcgccacg acctggccct gaatcttact
    16021 ggcacgcgct ttcagtttct gtttatcacg gattgtatga gacatggcaa cacctggtta
    16081 acaagaacat gaaaaatcat agcactatta atctactggg gggtagtatc aggtactggg
    16141 ggggagtaga atcagattgc cgaattaata ctaagaatta ttatcatgac cgaatttaca
    16201 actcttcttc agcaaggaaa cgcctggttc ttcatcccca gcgccatctt acttggtgcg
    16261 cttcatggcc tggaaccagg gcactcaaaa acgatgatgg cggcgtttat catcgccatc
    16321 aaaggcacca ttaaacaggc ggtgatgctc ggactggcag caaccatttc gcataccgcg
    16381 gtggtctggt tgattgcctt tggcgggatg gtgatcagta agcgctttac tgctcaatca
    16441 gcagaaccgt ggctccagct gatttccgca gtgatcatta ttggcaccgc gttctggatg
    16501 ttctggcgta cctggcgcgg cgaacgcaac tggctggaga atatgcacgg gcatgattat
    16561 gagcatcatc accacgatca cgaagatcac cacgaccatg gacatcatca ccatcacgaa
    16621 catggcgagt atcaggatgc ccatgcacga gcccatgcca atgacattaa acgacgcttt
    16681 gatggtagag aggtcaccaa ctggcaaatt ttgctatttg gcttaaccgg tggccttatc
    16741 ccctgcccgg cagcaattac cgtgctgttg atatgcattc agttgaaagc cctgacactg
    16801 ggcgcaacac tggtcgtcag tttcagcatt ggcctggcgt taacgcttgt caccgtaggc
    16861 gttggcgcag caatcagcgt tcagcaggtc gcaaaacgct ggagcggatt taacactctc
    16921 gctaaacgcg ccccctattt ttccagtctg ttgattggct tagttggtgt gtatatgggc
    16981 gtacatggct tcatgggcat aatgcgataa ctcaatcctt taggcttgca tgttatcttc
    17041 agcccaggat gattcttaaa tcagctattt ccgctgactc tctgctcgct aaggatgctt
    17101 ttagggcatc ctttcattta cactttttac gaaatcatgg gatcactaac aaaatatcgc
    17161 ttgtcagtta tattgtatgg caggaaagat atgcgactga tattacagat ccccaaagtg
    17221 gagagtttat gaccattaaa aataagatgt tgctgggtgc gcttttgctg gttaccagtg
    17281 ccgcctgggc cgcaccagcc accgcgggtt cgaccaatac ctcgggaatt tctaagtatg
    17341 agttaagcag tttcattgct gactttaagc atttcaaacc aggggacacc gtaccagaaa
    17401 tgtaccgtac cgatgagtac aacattaagc agtggcagtt gcgtaacctg cccgcacctg
    17461 acgccggaac ccactggacc tatatgggtg gcgcgtacgt gttgatcagc gacaccgacg
    17521 gaaaaatcat taaagcctac gacggtgaga ttttttatca tcgctaaaaa aagccccctc
    17581 ggagagaggg ggaaatgcag acaccttatt atttcttatt attagccact tgctcgtctt
    17641 gcttgttatt agtcgtattt cacgttgatt aatgcggttg cttccagtgc gccagattta
    17701 actttgtttg tatcgtagac gtagtaactg gctgttatcg gaattgaaac cgttgtatct
    17761 gtaagttttg actttgccgc aatttctttt aaatttgtgt atcctttact gctgttagtg
    17821 atatttaaaa gtgtgactgg cgaactatta ctttcaagaa taaatcctac gccattatct
    17881 tccgcacctt tcagtattgt gttgctgttg gttaacaagt cagaacttga taaatacacc
    17941 tgcattttcc gtgatgtttg attttgttga cctaataaat ttttacaacg cagaataata
    18001 tccccttgtt tactatttat tgttgctttg ttacctgtgg ctggtaattg tgaaacggga
    18061 atactgtcta aagtaattaa taaatcgtcc ggtttgcagg ttgtggttat cgggttgtat
    18121 gacaatgtca gattaaagga atagacacct gcattttgtg ccaatgcatt tctgtagcta
    18181 tttacacatg catcccatga cttgccacct aataaacaca gtagaaattg tactgcggca
    18241 ctcccgcgtg tcgatgatga agcactggat aatgttgcaa caccattaat catcactgaa
    18301 ccgccgcccc ccgcagaaac attcactttt gcattactgg ccgcaggtgt caccgttacc
    18361 gaataaccat tggtgaaact ggtctgatta tttgacaacg tgatgttatt tttattggtc
    18421 cattcaaaat gtaaatttaa tttaatggta tcgttataaa gtccaacaat aatatccgac
    18481 gggctcatag gggtgatagt atttgtactt acgcatgacg aatcattaaa attatgctca
    18541 tatgtcgcca tttgtgtttg agtaagtgca aaatccccct gaacaaccaa gttgtgctga
    18601 acagataact cactataaca tgccgcatag ctcacaggca taaccatcat cattaatata
    18661 aatagcatta tgcgaatttc catagaacca cctgactatt actggcaaat ataatttcta
    18721 ctttcatcaa tctctttacc gaaggtgatt gtgcatgaaa gtccttgttg cttatcaatt
    18781 gccacattaa ccgatggcgg tacttcattg gtgcgaataa ataactgact tccctggccg
    18841 acaacgccaa tattatgacc atggatatca ttgacttcat aaccaaacgt taatgattgc
    18901 ccatctgctc ttaacgcttt tataaaccat ggcttgcgct gatcggtatc aaaattaaca
    18961 agtacaaccg cgccgcgata aggggcggca attttccggt tgccacgtaa ttctgcttcg
    19021 ctatcgcttt gcgacacatc cagcatcagg tgattttccc gataaggtgt cattccgtcg
    19081 tatatcacca ctccattacg gtttgttgtg cgatattttt gcccattgac ataagcatct
    19141 ttaattcctg gcgcattcat cacagcaaac gtttcggaaa gacggttcgc cagattaacg
    19201 ccacccgacc aggcgacaat gccccctgaa acactggctc cagcctgtcg ataagtactc
    19261 gactgactat aactgccatt cactgtcgca accggcgcgt tccaggtcaa attcgcccca
    19321 gctgtcgttt catttccctg atgttgatga ctcaggttga caccataatt gaactgatcc
    19381 cgactcccta ctgttcctga taatcccgta ttatttgagg caaacccctg atcatcaaac
    19441 gtcgttgagt tagacatata tatttgccga cgaggcgtcg aaacgtcatc accccaatca
    19501 aagggaatcg atataaaaat attaaaacgt ttctcttcat gatgattctc gtcataagcc
    19561 tggcttgccg cgagggtata gcttatccgt cgcaggttgt tggaataact caactgataa
    19621 tccttactac tgccgctacg cccccagtaa tctcgccata acgtacttaa tgacacagac
    19681 ccccaacctt ctggcaatga ctggctcata ttggcggaaa agctattttt gcggccaaaa
    19741 tcgttctggt aataatcggc aatgtcatag acatcgtttt catcacggcg ataattatct
    19801 ttattgtttg cccaaacgtg atcgttaaat gtccggtaat cacgcgacga ataacgccag
    19861 gccgccagac caaaacgcgt cgacgtttgg ctcacaaatt tgttgtaggc aatttgataa
    19921 ctttgcccgt caaacacatc gccgttgtct tgtttactat gcgacttagt ggcatcgacg
    19981 gaaatggcac caatgcgtgt attccagcca gcccccaaag taaacgcgta ataattattc
    20041 gcgaccatcg agccaccata cagcgtcaat aaattattaa aaccatactg ataacccgcc
    20101 tggacaaaat cactttgttt gctcgcccct tcaatatggc tacgacccgc cgctaaatca
    20161 tatttcgaca cgccgggttg cagcatattt ggcaccgctg cataaggcac cagataggtg
    20221 gttaccgagc cgtccgcctc tttcacgctg acatcaagat ctgctccacc accggccaac
    20281 tgcaaatctg taatcgcgaa cgggccagga ggaacctctt tctgataaac cacaaaacca
    20341 ttctgttcaa tagttaccag cgcgttactc tgagcaatcc cctgcacccg tggcgtaaaa
    20401 ttttgtttcg agttaggcaa catctgcata tcacgaaaca accgcacacc tctgaagcga
    20461 acagaatcaa aaatatcgct tgatgtgtac atatcaccca cgcgaagcgt gccgagaagt
    20521 tgggcaaatc cacgttccag atacagggta ttgcttttcc acacccctgg attgttattt
    20581 gttttactga aactggcatc agaatgcagt tgccacccca gtaaatttaa cccgctgtta
    20641 aaacgtacat atgtactctt gttattaccc gacgctttat agtcgctgta atactgactc
    20701 agataataag aggtataaaa cgcattaata ccccgctccc agttttccgg tggaacatag
    20761 ccactttcca gttcttccac ccaggcctgc gggacactga aatcgagacg aaaaaccccg
    20821 atatcccagg tatagctccc accctgaaca agttgctcaa atgttaaaca ttgcttaccg
    20881 ctggcgaagt tatcgctatt aatgcctaac cgcttgataa cttctcttga taaacatgtt
    20941 tcttgcgggt tgtctttaac aataatctca tatttcccgc gccattgctt attgacataa
    21001 atatcgatgt catactgccc cggtaagggt tgattatcat caagacgaat atttgcaacc
    21061 tgctggtctt tcattccacc tatcataaaa tgggtatcaa aggtttcttc agctgcataa
    21121 gcttcaatgc cgagcaataa cgctacgatt gctgatgcaa gtggggtcat tctcaacata
    21181 ggcagctcct gcaattaaat tttgtcacta atatagttgc catggtcatc gataatggtc
    21241 agatgccagt tatttgcatt attacttttg acattaacac tctgactttc taagggggca
    21301 atcataatag tttcataatt gactttgaca ttattagctt tgacatccga aatcgtcacc
    21361 caattagctg agtcattttt tatcaccaaa ccattgccac tgcgatttac cagcaatttt
    21421 ttaaatgtcg ctttatttac cggagcaata cccgctggcc ggtaaaacaa cttaattctg
    21481 ttttgcatcg caaacttcag tgcattctta ccttcttgct ctggactatt tggtggaata
    21541 tccagaacat tcagataaaa aatgctttct ttattagtgg gcagtttatt cggcataatt
    21601 ttgattttta cttgctgccc ggaatttgcc cctatttttg ccactggtgg cgttaacatg
    21661 aaaggaacct gaattttttc tggtggtaat gacgtatcgc catcatcaat ccacgcctgc
    21721 agcagcgaag aacggtttcc ctggttcatc aactgcacca tcacttcttt attttctgcc
    21781 gggtaaataa tgcgcgtccc gtagataaca attccggcat gtgcaggaag gaccat
//
LOCUS       DAPHCV010000085        21761 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.085, whole genome shotgun sequence.
ACCESSION   DAPHCV010000085 DAPHCV010000000
VERSION     DAPHCV010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.085"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            234..569
                     /locus_tag="RH033_003245"
     CDS             234..569
                     /locus_tag="RH033_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HDV2992392.1"
                     /translation="MSLPRCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            729..1172
                     /gene="yjdN"
                     /locus_tag="RH033_003246"
     CDS             729..1172
                     /gene="yjdN"
                     /locus_tag="RH033_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HDV2992393.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1305..2093
                     /gene="phnC"
                     /locus_tag="RH033_003247"
     CDS             1305..2093
                     /gene="phnC"
                     /locus_tag="RH033_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HDV2992394.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLAHDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSYFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            2118..3134
                     /gene="phnD"
                     /locus_tag="RH033_003248"
     CDS             2118..3134
                     /gene="phnD"
                     /locus_tag="RH033_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV2992395.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKGNKGLNEQDKLAKTSAIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3240..4019
                     /gene="phnE"
                     /locus_tag="RH033_003249"
     CDS             3240..4019
                     /gene="phnE"
                     /locus_tag="RH033_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDV2992396.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4040..4765
                     /gene="phnF"
                     /locus_tag="RH033_003250"
     CDS             4040..4765
                     /gene="phnF"
                     /locus_tag="RH033_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HDV2992397.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGIAEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQMGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4766..5218
                     /gene="phnG"
                     /locus_tag="RH033_003251"
     CDS             4766..5218
                     /gene="phnG"
                     /locus_tag="RH033_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HDV2992398.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQTRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5215..5799
                     /gene="phnH"
                     /locus_tag="RH033_003252"
     CDS             5215..5799
                     /gene="phnH"
                     /locus_tag="RH033_003252"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HDV2992399.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSAPLSNDIVSQSLRFHTNAPLVNQPELATFAVTDEAIS
                     SEQLNALSSGTAVAPEAGATLILQVVSLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5799..6863
                     /gene="phnI"
                     /locus_tag="RH033_003253"
     CDS             5799..6863
                     /gene="phnI"
                     /locus_tag="RH033_003253"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HDV2992400.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGNTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSAQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6856..7701
                     /gene="phnJ"
                     /locus_tag="RH033_003254"
     CDS             6856..7701
                     /gene="phnJ"
                     /locus_tag="RH033_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HDV2992401.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMNMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWNEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            7698..8456
                     /gene="phnK"
                     /locus_tag="RH033_003255"
     CDS             7698..8456
                     /gene="phnK"
                     /locus_tag="RH033_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HDV2992402.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYGMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8567..9247
                     /gene="phnL"
                     /locus_tag="RH033_003256"
     CDS             8567..9247
                     /gene="phnL"
                     /locus_tag="RH033_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HDV2992403.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGEEWVDLVTAPARKVVEIRKTTIGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGVSS"
     gene            9244..10380
                     /gene="phnM"
                     /locus_tag="RH033_003257"
     CDS             9244..10380
                     /gene="phnM"
                     /locus_tag="RH033_003257"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HDV2992404.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGKIRAFAESQSRQPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTEAQMQQYEEEQLALAARWSQPNRETIAAM
                     CRARHIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10380..10937
                     /gene="phnN"
                     /locus_tag="RH033_003258"
     CDS             10380..10937
                     /gene="phnN"
                     /locus_tag="RH033_003258"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HDV2992405.1"
                     /translation="MTGKLIWLMGASGSGKDSLLTELRQREQTQLLVAHRYITRAASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10924..11358
                     /gene="phnO"
                     /locus_tag="RH033_003259"
     CDS             10924..11358
                     /gene="phnO"
                     /locus_tag="RH033_003259"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HDV2992406.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMHYHLALLDGEVVGMIGLHLQFHLHQVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKVL"
     gene            11360..12118
                     /gene="phnP"
                     /locus_tag="RH033_003260"
     CDS             11360..12118
                     /gene="phnP"
                     /locus_tag="RH033_003260"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HDV2992407.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECVACVRARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDQIPVYGPP
                     DEQGCDDLFKHSGLLDFSHTVEPFVMFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFDGMEIEVA"
     gene            complement(12146..12475)
                     /gene="yjdP"
                     /locus_tag="RH033_003261"
     CDS             complement(12146..12475)
                     /gene="yjdP"
                     /locus_tag="RH033_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HDV2992408.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12544..12993)
                     /gene="rpiB"
                     /locus_tag="RH033_003262"
     CDS             complement(12544..12993)
                     /gene="rpiB"
                     /locus_tag="RH033_003262"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HDV2992409.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERGVEVIDKGTWSSERTDYP
                     HYASQVALAVAGGEADGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQRRN"
     gene            13352..14242
                     /gene="alsR"
                     /locus_tag="RH033_003263"
                     /gene_synonym="rpiR"
     CDS             13352..14242
                     /gene="alsR"
                     /locus_tag="RH033_003263"
                     /gene_synonym="rpiR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="als operon DNA-binding transcriptional repressor
                     AlsR"
                     /protein_id="HDV2992410.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRSALEYYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
     gene            14301..15236
                     /gene="alsB"
                     /locus_tag="RH033_003264"
     CDS             14301..15236
                     /gene="alsB"
                     /locus_tag="RH033_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HDV2992411.1"
                     /translation="MNKYLKYFSGTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEGFVTTDNVAVGAKGASFIIDKLGAEGGEVA
                     IIEGKAGNASGEARRNGATEAFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANAGKTGKVLVVGTDGIPEARKMVEAGQMTATVAQNPADIG
                     ATGLKLMVDAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            15363..16895
                     /gene="alsA"
                     /locus_tag="RH033_003265"
     CDS             15363..16895
                     /gene="alsA"
                     /locus_tag="RH033_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
                     /protein_id="HDV2992412.1"
                     /translation="MATPYISMAGIGKSFGPVHALKSVNLTVYPGEIHSLLGENGAGK
                     STLMKVLSGIHEPTKGTITINNINYNKLDHKLAAQLGIGIIYQELSVIDELTVLENLY
                     IGRHLTKKICGVNIIDWREMRVRAAMMLLRVGLKVDLDEKVANLSISHKQMLEIAKTL
                     MLDAKVIIMDEPTSSLTNKEVDYLFLIMNQLRKEGTAIVYISHKLAEIRRICDRYTVM
                     KDGSSVCSGMVSDVSNDDIVRLMVGRELQNRFNAMKENVSNLAHDTVFEVRNVTSRDR
                     KKVRDISFSVCRGEILGFAGLVGSGRTELMNCLFGVDKRAGGEIRLNGKDISPRSPLD
                     AVKKGMAYITESRRDNGFFPNFSIAQNIAISRSLKDGGYKGAMGLFHEVDEQRTAEAQ
                     RELLALKCHSVNQNITELSGGNQQKVLISKWLCCHPEVIIFDEPTRGIDVGAKAEIYK
                     VMRQLADDGKVILMVSSELPEIITVCDRIAVFCEGRLTKILTNRDDMSEEEIMAWALP
                     QE"
     gene            16874..17854
                     /gene="alsC"
                     /locus_tag="RH033_003266"
     CDS             16874..17854
                     /gene="alsC"
                     /locus_tag="RH033_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HDV2992413.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSP
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGI
                     DPFLAALIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            17865..18560
                     /gene="alsE"
                     /locus_tag="RH033_003267"
     CDS             17865..18560
                     /gene="alsE"
                     /locus_tag="RH033_003267"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose-6-phosphate 3-epimerase"
                     /protein_id="HDV2992414.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDNHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLASKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RRHGMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKVAELKAW
                     REREGLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTAQIL
                     AAKSEVQPHAKTA"
     gene            18544..19473
                     /gene="alsK"
                     /locus_tag="RH033_003268"
     CDS             18544..19473
                     /gene="alsK"
                     /locus_tag="RH033_003268"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HDV2992415.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPD
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNPGCLETNCSGMALRRWYEQQPRNYPLSDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPAFPRETLIAMTQKYLRRPLPYQVV
                     RFIAASSSDFNGAQGAAILAHQRFLPQSCAKVP"
     gene            19746..21731
                     /gene="yjcS"
                     /locus_tag="RH033_003269"
     CDS             19746..21731
                     /gene="yjcS"
                     /locus_tag="RH033_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HDV2992416.1"
                     /translation="MNNSRLFRLSRIVIAFTAASGMMINTAYATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKIYYRANDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQMRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIIAVIYTHSHADHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVKALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQADNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMAVRLDSTKA
                     AGKNISLNFNMSNGDNLNLMLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         5182 a         6021 c         5838 g         4720 t
ORIGIN      
        1 aaaacatcat gtgaattaaa aaaagaacga gtagagcatt aaccttatct taaataataa
       61 atggaggcaa aaagattatt ttctttttgc gtttcctttc aaatgaaaac gatcgtcgtc
      121 taaaatcagc agtacccccg acaaactcag ggattttgtg tataattgcg gcctttttcg
      181 gcaatctgcc gttttttggc gcttttgccc tgctgacttt tgaggaaatc cacatgtcat
      241 taccacgctg cccaaaatgc aactccgaat acacctacga agataacggc atgtacatct
      301 gcccggaatg tgcctacgaa tggaacgacg cagaacctgc acaggaaagc gacgagctga
      361 tcgttaaaga tgctaacggc aatctgctgg ctgacggcga cagcgttacc atcattaaag
      421 atctgaaagt gaaaggtagc tcttcgatgc tgaaaattgg caccaaagtg aaaaacatcc
      481 gcctggttga aggcgaccat aacatcgatt gcaaaatcga cggttttggt ccgatgaaac
      541 tgaagtctga gtttgtgaaa aagaactgat tgtattgtga tcggtaggcc ggataaggcg
      601 tttacgccgc atccggcaac ggtggcgact gcctgatgcg acgctgaccg cgtcttatca
      661 ggcttccctc tcatctgtat aaatttcgaa ctacacttaa cgggcttctc ttaactgagg
      721 tcaccatcat gccgttaagt ccctacctct cttttgccgg taactgtgcc gacgcaattg
      781 cttattatca acgtacgttg ggcgctgaac tgctctataa aatcagcttc ggcgaaatgc
      841 caaaatcagc gcaggacagc gccgagaact gcccttccgg aatgcaattt cccgataccg
      901 ccatcgccca cgccaatgtg cgcattgccg gaagcgatat catgatgagc gatgctatac
      961 cgtcaggaaa agccagctac tccggcttta cgctggtgct cgattcgcaa caggtagaag
     1021 aaggaaaacg ctggtttgac aaccttgccg ctaacggaaa aatcgaaatg gcctggcagg
     1081 aaactttctg ggcgcatggc tttggcaaag tcaccgataa atttggcgta ccgtggatga
     1141 ttaatgtcgt caaacaacaa ccaacgcaat aatccgccgg gaggcccgcc ctcccgcact
     1201 gtcatcgaat tcccgttaac tcttcatctg ttagtcactt ttaattaacc aaatcgtcac
     1261 aataatccgc cacgatggag ccactttttt agggaggctg catcatgcaa acgattatcc
     1321 gtgtcgagaa gctcgccaaa accttcaatc agcatcaggc gctgcacgcg gttgatctga
     1381 acattcatca cggtgaaatg gtggctctgc ttgggccgtc gggttccggc aaatccaccc
     1441 ttttacgtca cttaagcggt ttgattaccg gcgataaatc tgccggtagc catatcgagc
     1501 tgctgggccg cacagtccag cgcgaaggcc gcctggccca cgatatccgc aaaagccgcg
     1561 ccaacaccgg ctacatcttc caacaattca acctggtgaa tcgcctgagc gtactggaga
     1621 acgtgctgat tggcgcgctc ggcagcacgc cattctggcg aacctgtttt agctacttca
     1681 cccgcgagca gaaacagcgc gcgttacagg cgctgacccg cgttggcatg gtgcactttg
     1741 cccatcaacg cgtttccaca ctctccggcg gccagcaaca gcgtgtggcg attgcccgcg
     1801 cgctgatgca gcaggcgaag gtgattctgg ccgatgagcc catcgcctcg ctggacccgg
     1861 aatcggcgcg catcgtgatg gacaccctgc gcgacatcaa ccagaacgac ggcatcaccg
     1921 tggtcgtcac gctacatcag gtggattacg ccctgcgcta ctgcgaacgc atcgtcgccc
     1981 tgcgtcaggg gcacgtcttt tacgacggca gcagccaact gtttgataac gaacgttttg
     2041 accatctcta ccgcagcatt aatcgcattg aagagaacgc gaaagctgcc tgacatcccc
     2101 atcattgagg aaaacgaatg aacgctaaga taattgcctc gctggccttc accagcatgt
     2161 tcagcctcag caccctgtta agcccggcgc acgccgaaga gcaggaaaag gcgctgaatt
     2221 tcggcattat ttcaacggaa tcacagcaaa acctgaaacc gcaatggacg ccgttcttac
     2281 aggatatgga gaagaagctg ggcgtaaagg tcaacgcctt ctttgccccg gactacgcgg
     2341 gcattatcca gggaatgcgc ttcaacaaag tggatatcgc ctggtacggc aatctgtcgg
     2401 cgatggaagc ggtggatcgc gccaacggtc aggtcttcgc ccagacggtc gcggcggatg
     2461 gatcgccagg ttactggagc gtgttgatcg tcaacaaaga cagtccgatc aacaacctga
     2521 acgatctgct ggcgaagcgt aaagatctca cctttggcaa tggcgatcct aactccacct
     2581 ctggcttcct cgtccccggc tactacgtct tcgccaaaaa caatatctcc gccagcgact
     2641 tcaagcgcac cgtcaacgcc gggcatgaaa ccaacgcgct ggccgtcgcc aacaaacagg
     2701 tggatgtggc gaccaacaac accgaaaacc tcgacaagct gaaaacctcc gcaccggaga
     2761 agctcaaaga actgaaggtg atctggaaat cgccgctgat cccaggcgat ccgatcgtct
     2821 ggcgcaaaaa tctgtccgaa accaccaaag acaagatcta cgacttcttt atgaactacg
     2881 gcaaaacgcc ggaagagaaa gcggtgctgg aacgcctggg ctgggccccg ttccgcgcct
     2941 ccagcgacct gcaactggtg ccgattcgcc agctcgcgct gtttaaagag atgcagggcg
     3001 tgaaaggcaa taaagggctg aatgagcagg acaagctggc aaaaaccagc gccattcagg
     3061 cgcaactgga tgacctggac cgcctgaaca acgcgttaag cgcgatgagt tcggtgagta
     3121 aagcggtgca gtaaatcgta ggtcggataa gacgccccgg cgtcgcatcc gacaatgtgc
     3181 agacgttgat gccggatgcg gtgcaagcac cttatccggc ctacagaccg gagccaaaca
     3241 tgcaaaccat caccatcgcc ccacccaagc gcagctggtt ctcgcttctg agctgggcca
     3301 ttgttctcgc cgtgttggtc gtctcgtggc agggcgcgga aatggccccg cttacgctga
     3361 tcaaagacgg cggcaacatg gcaaccttcg ccgccgactt cttcccgccc gatttcagcc
     3421 aatggcagga ttacctcacc gaaatggccg tcacgctgca aatcgccgtc tggggcaccg
     3481 cgctggcagt cgttctctcc atccccttcg gcctgatgag cgctgaaaac ctggtgccgt
     3541 ggtgggtgta tcagcccgtt cgccgcctga tggacgcctg ccgcgccatt aacgagatgg
     3601 tcttcgccat gctgttcgtg gtcgccgtcg gtctcggtcc gttcgctggc gtactggcgc
     3661 tgtttatcca caccaccggc gtgctctcca aactgctttc cgaagcggta gaagcaattg
     3721 aacccggccc ggtggaaggt attcgcgcca ctggtgccaa caagctcgaa gagatcctct
     3781 acggcgtgct gccgcaggtg atgccgctgc tgatctccta ctccctctat cgcttcgaat
     3841 ccaacgtccg ttcggccacc gttgttggca tggtcggcgc tggcgggatc ggcgtcaccc
     3901 tgtgggaagc gattcgcggt ttccagttcc agcaaacctg cgccctgatg gtgctgatta
     3961 tcgtcacggt cagcctgctg gatttcctct ctcaacggtt gcgtaagcac tttatctgat
     4021 aagcgaggca ttgatatcta tgcacttgtc tacacatccg accagctacc caacacgcta
     4081 tcaggagata gccgcaaaac ttgagcagga gcttcgtcaa cactaccgct gcggcgacta
     4141 tcttcccgcc gagcagcaac tggcggcgcg ctttgaggtg aaccgccaca ccctgcgccg
     4201 cgccattgac caactggtgg aaaaaggctg ggtacagcgc cgtcagggcg tcggcgtgct
     4261 ggtgctgatg cgcccgttcg attacccgct caacgcccag gcgcgtttta gccagaacct
     4321 gctggatcag ggcagccatc ccaccagcga aaaactgctg tctgtgctgc gcccggcctc
     4381 cggtcacgtc gctgacgcgc tggggatcgc cgagggcgag aacgttatcc acctgcgcac
     4441 cctgcgccgg gtcaacggcg tcgcgctctg tttaatcgac cactactttg cggacctcac
     4501 cctctggccg acgctgcaac gcttcgacag cggctcgctg cacgattttc tgcgcgagca
     4561 aatggggatt gcgctgcgcc gcagccagac gcggatcagc gcccgccgcg cccaggccaa
     4621 agagtgccag cgtcttgaaa tcccgaatat gtcgccgctg ctgtgcgtgc gcacccttaa
     4681 ccaccgtgac ggtgaaagca gcccggcgga gtactccgtc agcctgacgc gcgccgacat
     4741 gattgaattc actatggagc actgaatgca cgcagatacc gcgacccgcc agcactggat
     4801 gtccgtactg gcacacagcc aaccggctga actggcagcc cgcctgaacg cgctgaacat
     4861 caccgccgac tatgaggtga tccgcgccgc tgaaaccggc ctggtgcaga ttcaggcgcg
     4921 gatgggtggc accggcgaac gcttttttgc tggcgacgcc acgctcaccc gcgccgccgt
     4981 acgcctgact gacggcacgc tcggctacag ctgggtgctg gggcgcgaca aacagcacgc
     5041 cgaacgctgc gcgctgattg acgcgctgat gcagcaaacc cgccattttc aaaacttatc
     5101 agaaaccctt attgccccgc tggacgctga ccgtatggca cgcattgccg cacgccaggc
     5161 cgaagtgaac gccagccggg tcgacttctt tacgatggtt cgcggagaca acgcatgacc
     5221 ctggaaaccg cttttatgct tcccgtgcag gatgcccagc acagttttcg tcgcctgtta
     5281 aaggccatga gcgagccggg cgtgattgtc gccctgcatc agctcaaacg cggctggcag
     5341 ccgctgaata tcgccaccac cagcgtgctg ctgacgctgg ccgataacga cacgccggtg
     5401 tggctttctg cgccattaag taacgatatc gtcagccaga gcctgcgttt tcataccaac
     5461 gcgccgctgg tcaatcagcc ggaactggcg accttcgcgg taacggatga ggcgatttcc
     5521 agcgaacagc tcaacgctct ttccagcggc accgctgttg cgccggaagc gggcgcgacg
     5581 ctgattttac aggtcgtcag cctgagcggc gggcgcatgc tgcgccttac tggcgcgggt
     5641 attgccgaag aacgaatgat cgccccgcag ctgccggagt gcattctgca cgaacttacc
     5701 gagcgcccgc acccgttccc gctcggcatc gacctgatcc tgacctgcgg cgagcggctg
     5761 ctggctatcc cgcgaaccac tcatgtggag gtgtgctgat gtacgttgcc gtgaaagggg
     5821 gcgagaaggc gatcgacgcc gcccacgccc tgcaagagag ccgacgccgg ggcaataccg
     5881 atttgcctga actgagcgtc gcccagattg aacagcagct taatcttgca gtagatcgcg
     5941 tgatgaccga aggcggcatt gccgaccgcg aactggcggc gctggcgctg aaacaggcca
     6001 gcggcgataa cgtcgaagcg attttcctgc tacgcgccta ccgcaccacg ctggcgaagc
     6061 tggcggtaag cgagccgctc gacaccacgg agatgcgtct cgaacggcgt atttcagcgg
     6121 tttataaaga tattcccggc ggccagctgc ttggcccaac ctacgactac acccatcgcc
     6181 tgctcgattt caccctgctg gcaaacggcg aagcgccgac gctgaccacc gccgacagcg
     6241 cacaacagcc gtcgccgcac gttttcagcc tgctggcgcg tcaggggctg gcgaagtttg
     6301 aagaagatag cggcgcacag ccggatgaca tcacccgcac gccgccggtt tacccctgct
     6361 cacgctcctc ccgcctgcaa caattaatgc gcggggacga aggctatttg ctggcgctgg
     6421 cctactccac ccagcgtggt tacgggcgca atcacccgtt cgcgggcgag atccgcagtg
     6481 gctatatcga cgtgtctatt gtgccggaag agctgggatt tgcggtgaac gtcggcgaac
     6541 tgctgatgac cgaatgtgaa atggtcaacg gttttatcga cccgccggat gagccgccgc
     6601 acttcacgcg cggctacgga ctggtgttcg gcatgagcga gcgcaaagcg atggcaatgg
     6661 cgctggttga ccgtgcgttg caggctccgg agtacggcga gcacgcgaca ggcccggcgc
     6721 aggatgaaga gttcgtgctg gcgcatgccg acaacgtcga agccgcaggc tttgtctcgc
     6781 accttaaact cccccactac gtcgatttcc aggccgaact ggagctactc aaacgtctgc
     6841 aacaggagca gaaccatggc taatctgagc ggctacaact ttgcctacct cgacgagcag
     6901 accaaacgca tgatccgccg tgccatctta aaagcggtgg cgatccccgg ttatcaggtg
     6961 ccgtttggcg ggcgcgagat gccgatgccg tacggctggg gaaccggcgg catacagctt
     7021 accgccagcg tgattggcga aagcgatgtg ctgaaggtga ttgaccaggg cgcggacgac
     7081 accaccaacg cggtgtcgat ccgtaacttc tttaagcgcg tgaccggggt aaacaccact
     7141 gaacgtacgg acgatgcgac gcttatccag acgcgccacc gcatccccga aacgccgctg
     7201 accgaagatc agatcattat tttccaggtg ccgatccccg aaccgctgcg ctttatcgag
     7261 ccgcgcgaaa cggaaacccg caccatgcac gcgctggagg agtacggcgt gatgcaggtg
     7321 aagctgtatg aagatatcgc ccgcttcggt catatcgcca ccacctacgc ctatccggtg
     7381 aaggtaaacg ggcgctacgt aatggacccg tcgccgatcc cgaaatttga taacccgaaa
     7441 atgaacatga tgcccgccct gcaactgttc ggcgcggggc gcgagaagcg catctatgcg
     7501 gtgccgccgt ttacccgcgt ggaaagcctc gatttcgacg atcacccgtt caccgttcag
     7561 cagtggaatg agccatgcgc catctgcgga tcgacccaca gctatcttga tgaagtggtg
     7621 ctggatgacg ccggaaaccg catgtttgtc tgctccgata ccgattattg ccgccaacag
     7681 agcgaggcga aaagccaatg aatcaaccgt tactttcggt caataacctg acccaccttt
     7741 acgcgccggg caaaggcttt agcgatgtct cttttgattt atggccgggg gaagtgctgg
     7801 gcatcgtcgg ggaatccggc tccgggaaga ccacgctgct gaagtcgatc tccgcgcgcc
     7861 tgacgccgca gcaaggggaa attcgctatg aaaaccgttc cctctatggc atgagcgagg
     7921 ccgaccgccg tcgcctgctg cgcaccgaat ggggcgtggt gcatcagcat ccactcgacg
     7981 gcctgcgccg ccaggtgtcg gcaggcggca atatcggcga gcggctgatg gcgaccgggg
     8041 cgcgtcatta cggcgatatt cgtgccaccg cgcagaagtg gctggaagag gtggaaattc
     8101 ccgccaaccg gatcgacgac ctgccgacca ccttttccgg cggtatgcag cagcgtttgc
     8161 agattgcccg caacctggtg acgcatccga agctggtatt tatggacgag ccaaccggcg
     8221 ggctggatgt atctgtgcag gcgcgcctgc tcgacctgct gcgcggcctg gtggtggagc
     8281 tgaacctggc ggtggtgatt gtcacccacg atttaggcgt cgcccgcctg ctggcggacc
     8341 gtttgctggt gatgaagcag gggcaagtgg tggagagtgg gttaaccgac cgcgtgctcg
     8401 acgacccgca ccatccgtat acacaactgc tggtgtcatc ggttttgcag aattgagccg
     8461 gtgccggatg cggcgtaaac gccttatccg gcctacaaat gcgctccccg taggtcggat
     8521 aagacgcgtc agcgtcgcat ccgacacccg aaccacgagg cgaaaaatga ttaacgtaca
     8581 aaacgtcagt aaaaccttca tcctgcatca gcaaaacggc gtgcgcctgc ccgtcttaca
     8641 acgtgcctcg ctcaccgtca acgcaggcga atgcgtggtg cttcacggcc actccggcag
     8701 cggaaaatca accctgctgc gctcgctgta cgccaactat ctgcccgacg aagggcaaat
     8761 ccagatcaaa cacggtgagg aatgggtaga cctggtcacc gcgccagccc gcaaagtggt
     8821 ggagatccgc aaaaccacca tcggctgggt gagccagttt ctgcgcgtca tcccgcgtat
     8881 ttcggcactg gaagtggtga tgcagcctct gctcgatacc ggcgtaccgc gtgaagcctg
     8941 cgccgctaaa gccgcccgat tactgacgcg cctgaacgtg cctgaacgcc tgtggcacct
     9001 ggcaccatcg accttctccg gcggcgaaca gcagcgcgtc aacatcgccc gtggctttat
     9061 cgtcgactat ccgattctgc tgctggatga acccaccgcc tcgctggacg ctaaaaacag
     9121 cgccgcggtg gtggaattaa ttcgcgaagc caaagcccgt ggcgcggcca tcgtcggtat
     9181 cttccatgac gaagccgtac gcaatgacgt cgccgaccgc ctgcacccaa tgggagtctc
     9241 ttcatgatta tcaataacgt caaactggtg ctggaaaacg aagtggtgca cggttcactg
     9301 gaagtacagg atggcaaaat tcgcgccttt gccgaaagcc agagccgcca gccggaagcg
     9361 atggacggcg aaggtggctg gctgctgccg gggctgattg agctgcatac cgataatctg
     9421 gataaattct tcaccccgcg tccaaaagtg gactggcccg cccactcggc aatgagtagc
     9481 cacgacgcgc tgatggtggc gagcggcatc accaccgtgc tggacgcggt ggcgattggc
     9541 gacgtgcgcg acggcggcga tcggctggag aatctggaga agatgatcaa cgccatcgaa
     9601 gagacgcaga aacgcggcgt caaccgcgcc gagcaccgtc tgcatctgcg ctgcgaactg
     9661 ccgcatcaca ccacgctgcc gctgtttgaa aaactggtgc agcgcgaacc ggtgacgctg
     9721 gtgtcgctga tggatcactc gccgggccag cgccagttcg ctaaccgcga gaagtatcgc
     9781 gaatattatc agggcaaata ctcgctcacc gaagcgcaga tgcagcagta tgaagaagag
     9841 cagctggcgc tcgccgcacg ctggtcgcag ccaaatcgcg aaaccatcgc cgcaatgtgc
     9901 cgcgcgcgac acattgcgct cgccagccac gatgacgcca cgcacgccca cgttgctgaa
     9961 tctcaccaac ttggcagcgt aatcgccgaa tttcccacca cgttcgaagc ggcagaagcc
    10021 tcgcgcaagc atggcatgaa cgtgctgatg ggcgcaccga atatcgtgcg cggcggttcg
    10081 cactccggca acgtggcggc cagtgaactg gcgcagcttg gcctgctgga tatcctctct
    10141 tccgactact accccgccag cctgctcgat gcggcatttc gcgtcgccga tgacgagagc
    10201 aaccgcttta cgctgccgca ggcggtgaag ctggtgacta aaaatccggc gcaggcgctt
    10261 aatcttcagg atcgcggggt gattggcgag ggcaaacgcg ccgacctggt gctggcgcat
    10321 cgcaagggca atcacattca tatcgaccac gtctggcgtc agggtaaaag ggtgttctga
    10381 tgacgggaaa actgatttgg ttaatggggg cctctggctc cggaaaagac agtctgttga
    10441 cggaactccg ccagcgggaa caaactcagc tactggtagc gcatcgctac atcacgcgcg
    10501 ccgccagcgc cggaagtgaa aaccatatcg ccctgagcga gcaggagttt tttacccgcg
    10561 cggggcagaa ccttctggcc ttaagctggc acgctaacgg tctgtattat ggcgtcggcg
    10621 tcgagattga cctctggctg cacgctggat ttgacgtggt ggtcaacggc tcacgcgccc
    10681 atctgccgca ggcgcgggcg cgctatcaat cggcgctgct gcccgtttgt ttacaggttt
    10741 cgccagagat cctgcgccag cgcctggaaa accgtggtcg tgaaaatgcc agtgaaatta
    10801 acgcccggct ggcgcgcgct gcccgctata ctcctcagga ttgtcttacg ctcaataatg
    10861 acggcagcct gcgccagtcg gtcgacacgc tgctgacgct gattcatcag aaggagaaac
    10921 accatgcctg cttgtgagct tcgcccggcc acgcagtacg ataccgacgc agtttacgcg
    10981 ctgatttgtg agctaaaaca ggcggagttt gaccaccacg cgtttcgcgt gggctttaac
    11041 gccaacctgc gcgacccgaa catgcactac catctggcgc tgctggatgg cgaagttgtc
    11101 ggcatgatcg gcctgcattt gcagtttcat ctgcatcaag tcaactggat cggcgaaatt
    11161 caggaactgg tggtgatgcc gcaggcgcgc ggtctgaatg tcggcagtaa attactggca
    11221 tgggcagaag aagaagcccg ccaggccggg gccgaaatga ccgagctttc caccaacgtc
    11281 aagcgccacg acgcgcaccg tttctatctg cgcgaaggct acgagcagag ccacttccgc
    11341 ttcaccaagg tgctgtaaca tgagcctgac cctcacactt actggcactg gcggcgcaca
    11401 gggcgttccc gcatggggct gtgagtgcgt ggcctgcgtc agagcgcggc gctcaccgca
    11461 gtatcgccgc cagccatgca gcggcgtcgt gaagtttaac gacgcgatca ccctgatcga
    11521 cgccgggcgg cacgatctcg ccgatcgctg gtcgcccgga tcgttccagc agtttttgct
    11581 gacgcactac catatggatc acgtgcaggg gctgtttccg ctgcgctggg gcgtgggcga
    11641 tcagatcccg gtctacggcc cgccggatga acagggctgc gacgatctat tcaaacattc
    11701 gggcctgctt gatttcagcc acacggtgga gccatttgtg atgtttgatt tgcagggatt
    11761 acaggtcacg ccgctgccgc tcaaccattc gaaactcact ttcggctatc tactggaaac
    11821 ggcacatagc cgggtggcgt ggctgtctga caccgcaggc ctgccggaaa aaacgctgaa
    11881 atttttacgc aataatcagc cacaggtaat ggtgattgat tgcagccacc cgccgcgcga
    11941 ggatgcaccg cgtaatcact gtgatttaaa taccgtgctt gcgctgaatc aggttatccg
    12001 ctcgccacgg gtgatactga cccatatcag ccaccagttt gatgcgtggc tgatggaaaa
    12061 cgcactgccg tcggggtttg aggcggggtt tgatgggatg gagattgagg tggcgtgagg
    12121 gaaaataccc gcgccggccc ctatatcaac gcccatactc atcttccatt ctccgctctt
    12181 catcctccag ttgccgacgc tcctgatcaa gctggcgctg gcgatcgtcc agctgcctgc
    12241 ggcgatcttc aaactggcgg cggcggtcgt catattgtct gcgccgatcg tcgctcactt
    12301 cacgctgcca gccgtcgtcg cgcgaatctt catagtctcg cccacggtca gggttataag
    12361 cgtcgttaat cgcctgctga atattgccaa tggtgtcgtc gataatatcg gcctgggccg
    12421 gaacggtgga cagcgtgagc agggtgaata aaagaaatag cggaaagcgt ttcattagcc
    12481 aacctcaaaa agaaactcta tccacattag tcattactca tccatgcaag tagtggacga
    12541 atctcaattt ctccgctgct ctattgccgt aatcgcctcc acgcgttgtt gatgacgacc
    12601 gccttcgtac tgtgcgccca gccacgcatc gacaatcatt tttgccagtt cgaggccaac
    12661 cactcgtgaa ccaaaagcca gcacgttggt gtcgttatgc tgccgcgaaa gttgcgcgga
    12721 ataaggttcg ctacaaacga ccgcgcgaat tccggcaaac ttgttcgccg ctatcgaaat
    12781 accgacgcca gtgccacaaa tcaaaatccc gccatcagcc tctccgccag caactgccag
    12841 tgcgacttga ctggcgtaat gcggataatc agtacgctct gacgaccagg ttcctttatc
    12901 aatcacttca acgccacgct caactaaatg tgccactatt tcatgtttta aaatgaaacc
    12961 gacatgatca cagccaaatg caatcttttt cattcatctt ccccgcccgc gcaaaacttc
    13021 acaatcaaaa aacatttgtg atgtaaatga attaaaaaca cccaaaatcg atgaaatccg
    13081 ccttcctgat ctgttttaca gagttacatc tggcagcgca atagcgttaa agacactcac
    13141 tatccagcaa catctatcat ccaataaacc agaaaaacaa ataacatcat gtttttaaag
    13201 tgattaaatg aaatgaaatt ttaagccact ctccattatt cacaacaaaa caaactttat
    13261 aaattttatt tttttgtgaa gttgccagca tcttttctgt tcttgctgtg gtgatatagt
    13321 ggcgtcttca attcaaggac aagagaacgt gatgagccag tcagagtttg attcagcgct
    13381 tccgaacggt atcgggttag cgccttacct gcgaatgaag caggaaggaa tgacagaaaa
    13441 cgaaagccgc atcgtggagt ggttactcaa acccggtaac ctgagttgtg cacccgcaat
    13501 taaagatgtc gcagaagctc tggcggtatc tgaagcgatg atcgttaagg tatcaaagct
    13561 gctggggttt agcggctttc gtaacttacg cagtgcgctg gaatattatt tttctcagtc
    13621 agaacaagta ttgccttccg agttggcttt tgatgaagcg ccgcaggatg tggtgaataa
    13681 agtatttaac atcactttac gcaccattat ggaaggtcag tcgatcgtca acgttgatga
    13741 gatccaccgt gccgcccgct ttttctatca ggccagacag cgggatttgt acggtgccgg
    13801 aggatcaaat gctatctgtg ctgatgtaca gcacaagttt ttacgcattg gcgtacgctg
    13861 ccaggcctat ccggacgctc acatcatgat gatgtctgct tcgttgttac aggaaggaga
    13921 tgtggtgctg gtggtgaccc attccgggcg aaccagtgat gtaaaagcgg ctgtagaact
    13981 ggcaaaaaag aacggggcaa agattatttg tataacgcat agctaccatt caccgatagc
    14041 gaagctggcc gattatatta tttgctcacc ggccccggaa acaccgttat taggtcgtaa
    14101 tgcctcggca agaatattac aactaacttt gctggacgcg ttttttgtct ctgtcgccca
    14161 gctcaacatt gaacaagcta atattaatat gcaaaaaacc ggcgcaattg ttgatttctt
    14221 ctcaccaggc gcgctgaaat aagaaaaagg taattccgcc atcgcggagt tacctctccc
    14281 cttaccgtga gaacatgatt atgaataaat atctgaaata tttcagcggc acactcgtgg
    14341 gcttaatgtt gtcaaccagc gcttttgctg ccgccgaata tgccgtcgta ttgaaaacac
    14401 tctccaatcc attttgggta gatatgaaaa aaggcattga agatgaagca aaaacgctgg
    14461 gcgtcagcgt tgatattttt gcctctcctt cagaaggcga ttttcaatct caattgcagt
    14521 tatttgaaga tctcagtaat aaaaattaca aaggtatcgc cttcgcacca ttatcctcag
    14581 tgaacctggt catgcctgtc gcccgcgcat ggaaaaaagg catttatctg gtcaatctcg
    14641 atgaaaaaat cgacatggat aatctgaaaa aagcaggcgg taatgtggaa ggttttgtca
    14701 ccaccgataa tgttgccgtc ggggcgaaag gcgcgtcatt cattattgac aagctgggtg
    14761 ccgaaggggg tgaagtcgca atcattgagg gtaaagccgg taacgcctcc ggtgaagcgc
    14821 gtcgtaatgg tgccaccgaa gccttcaaaa aagcaagcca gatcaagctt gtcgccagcc
    14881 agcctgccga ctgggaccgt attaaagcac tggatgtcgc cactaacgtg ttgcaacgta
    14941 atccgaatat taaagcgatc tattgcgcga atgacacgat ggcaatgggt gttgctcagg
    15001 cagtcgcaaa cgccggaaaa acgggcaaag tgttggtcgt cggtacagat ggcattccgg
    15061 aagcccgcaa aatggtggaa gccggacaaa tgaccgcgac ggttgcccag aacccggcgg
    15121 atatcggtgc aacgggtctg aagctgatgg ttgacgctga gaaatccggc aaggttatcc
    15181 cgctggataa agcaccggaa tttaaactgg tcgattcaat cctggtcact caataaaact
    15241 gaatgatgcc ggatgatgat gctaatgaag tgtcttatcc ggccaacaaa ataacctgaa
    15301 cgtagacctg ataagtgtag ccatcaggca ataatacgat atttcgtgca gagaggttat
    15361 ttatggccac gccatatata tcgatggcgg ggatcggcaa gtcctttggt ccggttcacg
    15421 cattaaagtc ggttaattta acggtttatc ctggtgaaat acattcatta ctaggagaaa
    15481 atggcgcggg taaatccacg ctaatgaaag ttttatccgg aatacatgag ccgaccaaag
    15541 gcaccattac cattaataac attaactata acaaactgga tcataaatta gcggcacaac
    15601 tcggcatcgg gattatttat caagaactca gcgttattga tgaattaacc gtactggaaa
    15661 atttatatat tggtcgtcat ctgacgaaaa aaatctgtgg cgtcaatatt atcgactggc
    15721 gagaaatgcg tgtccgcgcc gccatgatgt tattacgcgt gggcttgaaa gttgatttag
    15781 atgagaaagt ggcgaattta tctatcagcc acaagcagat gctggaaatt gccaaaacgc
    15841 tgatgctcga cgccaaagtc atcatcatgg atgaacccac ctcctcactc accaataaag
    15901 aggtggacta tttgtttctg atcatgaatc agttgcgtaa ggagggtacg gccatcgtct
    15961 atatctcgca taaattggcg gaaattcgcc gtatttgcga ccgctatacg gtgatgaaag
    16021 acggcagcag cgtttgcagc ggcatggtga gcgatgtgtc aaatgacgat atcgtccgtc
    16081 tgatggtagg tcgcgaactg caaaaccgtt ttaacgcgat gaaggagaat gtcagcaacc
    16141 ttgcgcacga tacggttttt gaggtgcgca acgtcaccag ccgtgacaga aaaaaggtcc
    16201 gggatatctc atttagcgtc tgccggggag aaatattagg ctttgccgga ctggtcggtt
    16261 ccggacgtac tgaactgatg aactgcctgt ttggcgttga taaacgcgct ggcggagaaa
    16321 tccgtcttaa tggcaaagat atctctccac gttctcccct ggatgccgtg aaaaaaggga
    16381 tggcttacat cactgaaagc cgccgggata acggcttttt ccccaacttt tctatcgctc
    16441 agaacatagc tatcagccgc agtctgaaag acggcggcta taaaggcgcg atggggttgt
    16501 ttcatgaagt tgacgagcaa cgtaccgctg aagcccagcg cgaactgctg gcgctgaaat
    16561 gtcattcggt aaaccagaac atcactgaac tctccggcgg taaccagcag aaagtcctga
    16621 tctccaaatg gctgtgctgt cacccggaag tgatcatttt cgatgaacct acccgtggca
    16681 tcgacgttgg cgcgaaagcc gaaatttaca aagtgatgcg ccaactggcg gacgacggaa
    16741 aagtcatcct gatggtgtca tctgaattac ctgaaattat caccgtctgc gaccgcatcg
    16801 ccgtgttctg cgaaggacga ctgacgaaaa tcctgacgaa tcgcgatgac atgagcgaag
    16861 aggagattat ggcatgggct ttaccacaag agtaaaaagc gaagcgagcg agaagaaacc
    16921 gttcaacttt gcgctgttct gggataaata cggcaccttt tttatcctgg cgatcatcgt
    16981 cgccattttt ggttcgctgt cgccagaata ttttctgacc accaataata ttacccagat
    17041 ttttgtgcaa agctccgtga cggtactgat cggcatgggg gaattcttcg ctatcctggt
    17101 cgccggtatc gacctttcgg ttggcgcgat tctggcgctt tccggtatgg tgaccgctaa
    17161 actgatgttg gcaggtattg atccgtttct cgcggcgcta attggcggtg tactggttgg
    17221 cggcgcactg ggggcgatca acggctgcct ggtcaactgg acgggactac acccgttcat
    17281 tatcaccctt ggcaccaacg ctattttccg tgggatcacg ctggtgatct ccgatgccaa
    17341 ctcggtatac ggcttctcat ttgacttcgt gaacttcttt gccgccagcg taattgggat
    17401 acctgtcccc gttatcttct cgctaattgt cgcgctcatc ctttggtttc tgacaacgcg
    17461 tatgcggctc ggacgcaaca tctacgcact gggcggcaac aaaaactcgg cgttctattc
    17521 cgggattgac gtgaaattcc acatcctggt ggtgtttatc atctccggtg tttgtgcagg
    17581 tctggcaggc gtcgtctcaa ctgcacgact cggtgccgca gaaccgcttg ccggtatggg
    17641 gtttgaaacc tatgccattg ccagcgccat cattggcggc accagtttct tcggcggcaa
    17701 ggggcgcatt ttctctgtgg tgattggcgg gttgatcatc ggcactatca acaacggtct
    17761 gaatattttg caggtacaaa cctattacca actggtggtg atgggcggat taattatcgc
    17821 ggctgtcgcc cttgaccgtc ttatcagtaa gtaaggaatt gaacatgaaa atctccccct
    17881 cgttaatgtg tatggatctg ctgaaattta aagaacagat cgaatttatc gacaaccatg
    17941 ccgattactt tcacatcgat atcatggacg gccactttgt gccgaatctg acgctctcac
    18001 cgttcttcgt aagccaggta aaaaaactgg cgagtaaacc gctcgactgc catctgatgg
    18061 taacgcgtcc gcaggattac attgctcaac tggcacgtgc aggagctgat ttcatcactt
    18121 tgcatccgga aactatcaac ggtcaggcat tccgcctgat tgatgaaatc cgccgtcatg
    18181 gcatgaaagt ggggctgatt cttaatccgg aaacgccggt tgaggccatg aaatactata
    18241 tccataaggc tgataaaatt acggtcatga ctgtcgatcc cggttttgcc ggacaaccct
    18301 tcattcctga aatgctggat aaagttgccg aactgaaggc atggcgtgaa cgagaaggtc
    18361 tggagtacga aattgaggtg gacggttcct gcaaccaggc aacttacgaa aaactaatgg
    18421 cggcaggggc ggatgtcttt atcgtcggca cttccggcct gtttaatcat gcggaaaata
    18481 tcgacgaagc atggagaatt atgaccgcgc agattctggc tgcaaaaagc gaggtacagc
    18541 ctcatgcaaa aacagcataa cgtcgtagcg ggcgtggata tgggggcaac gcatatccgc
    18601 ttttgtctgc ggacagcaga aggtgaaacg ctacactgcg aaaaaaagcg gaccgcagaa
    18661 gttattgctc ccgacctggt gtcgggtatc ggcgaaatga ttgacgagca actcaggcgc
    18721 tttaacgctc gctgtcatgg tctggtgatg ggatttccgg cgctggtcag taaagataaa
    18781 cgcaccatta tttctacgcc taacctgccg ttaacagcgg cagatttata tgatctcgcc
    18841 gataagctcg aaaatacgct gaattgtccg gttgagtttt cccgcgacgt taacctgcaa
    18901 ctctcctggg acgtagtaga aaatcgcctt acgcaacaac tggttctggc agcctatctc
    18961 ggtacgggga tggggttcgc agtgtggatg aacggtgcgc cgtggacggg tgcgcacggt
    19021 gtggcaggcg aactgggcca tatccccctg ggagatatga cccaacactg cgcgtgtggt
    19081 aatcctgggt gcctggaaac caattgctct ggaatggcgc taagacgctg gtacgaacaa
    19141 cagccccgaa attacccatt gagcgatctt ttcgtccatg cggaaaacgc ccctttcgtc
    19201 cagagtctgc ttgaaaacgc ggcacgagcc attgccacca gcattaatct gttcgatccc
    19261 gatgcggtga ttctgggcgg tggcgtgatg gatatgcccg ccttcccacg cgagactctc
    19321 attgccatga cccaaaagta cctgcgccgt ccactgccgt atcaggttgt gcgctttatt
    19381 gccgcctcat cttctgactt taatggcgct cagggtgcag caatattggc gcatcaacgt
    19441 tttttgccac agtcctgtgc taaagtccca tgagtaaagc gttggcacag tagcgcaatg
    19501 tcccgctgtg ccaacgacag gtacaatata tataataatt acataaaaca gcccatatta
    19561 ttcccagcaa tgatagttct actgtaattt tccctcttca gcacaaatgc ctaataaaat
    19621 ggctcgttcc gctcacactt ccacttatta aagtcgcaat ttcagaatta tgataacgcc
    19681 agctttaaat gacgtatttt gcatggatta catatttacg acataaatga taaatggaga
    19741 tttatatgaa taactctcgg ttattccgtt tgagcaggat tgttattgcg ttcactgccg
    19801 ccagcggcat gatgataaat accgcttacg caacagatga agcgaaagcc gccactcaat
    19861 atacccaaca ggttaatcag aattacgcca aatcattacc gtttagcgat cgtcaggatt
    19921 ttgacgatgc ccagcgtgga tttatcgctc cgctgctgga tgaaggtatt ctgcgtgatg
    19981 cgaacggtaa aatttactac cgcgcgaatg attacaaatt tgatattaat gccgctgcgc
    20041 cggaaaccgt aaaccccagc ctgtggcgtc agtcgcagat caacggcatt tcaggcctgt
    20101 tcaaagtcac cgataaaatg tatcagatgc gcggccagga tatctctaac attacgttcg
    20161 ttgagggcga gaaaggcatt attgttattg acccactggt gacgccacct gccgcaaaag
    20221 ccgcacttga cctttacttc cagcatcgtc cgcaaaaacc cattattgcc gttatctata
    20281 cccacagcca cgccgaccac tatggcggcg tgaaaggcat catctctgaa gccgatgtta
    20341 aatccggcaa agttcaggtg attgctccag ctggctttat ggacgaagcc atcagcgaaa
    20401 acgtgctggc gggtaacatc atgagccgcc gtgcgctcta ctcttacggt ctgttactgc
    20461 cgcacaacgc gcaaggcaac gtgggcaacg gcctgggcgt gacgctggca acgggcgacc
    20521 cgagcattat tgcaccgact aaaactatcg tcagaactgg cgagaagatg attatcgacg
    20581 gcctggaatt tgacttcctt atgacccctg gtagcgaagc gccagccgaa atgcacttct
    20641 atattccggc cctgaaagcc ctgtgtaccg ccgagaacgc cacgcatacc ctgcacaact
    20701 tctacactct gcgcggcgcg aaaacccgcg ataccagcaa gtggaccgag tacctgaacg
    20761 aaacgctgga tatgtggggt aacgacgcgg aagtactgtt tatgccgcac acctggccgg
    20821 tctggggcaa taagcatatc aatgattata ttggtaaata tcgcgatact atcaagtaca
    20881 ttcacgacca gaccctgcac ctggcgaacc agggctacac catgaatgaa atcggcgaca
    20941 tgattaagct gccgcctgcg cttgccaata actgggccag ccgcggctat tacggttctg
    21001 tcagccacaa cgcccgcgcg gtgtataact tctatcttgg ctattacgac ggtaacccgg
    21061 ctaacctgca tccgtatggt caggtggaga tgggtaaacg ttacgtgaaa gcgctgggcg
    21121 gttctgcccg cgtcatcaac ctggcgcaag aagcgaacaa gcaaggtgat taccgctggt
    21181 cggcagaact gctgaaacag gtgattgccg ccaacccggg tgaccaggtc gcgaagaatc
    21241 tgcaagcgga taactttgaa cagctgggct atcaggccga gtccgccaca tggcgcggtt
    21301 tctacctgac cggcgcgaaa gagctgcgcg aaggggtaca taagttcagc cacggcacca
    21361 ccggttcccc ggacaccatt cgtgggatgt cggtcgaaat gctgttcgac tttatggccg
    21421 ttcgcctcga tagcacgaaa gccgcaggta aaaatatcag cctgaacttc aatatgagca
    21481 acggcgataa cctcaacctg atgctgaacg atagcgtgct taactaccgt aaaacgctgc
    21541 aatcgcaagc cgacgcctct ttctacatta gccgtgaaga tctgcacgcc gtgctgaccg
    21601 gacaggctaa aatggccgat ctggtgaaag cgaagaaagc caaaattatt ggcaatggcg
    21661 cgaaactgga agaaattatc gcctgtctgg ataatttcga tttgtgggtg aatatcgtaa
    21721 ccccaaatta atgtcactcg cttttggggg cgctgaagcc c
//
LOCUS       DAPHCV010000086        21631 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.086, whole genome shotgun sequence.
ACCESSION   DAPHCV010000086 DAPHCV010000000
VERSION     DAPHCV010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.086"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            75..566
                     /locus_tag="RH033_003270"
     CDS             75..566
                     /locus_tag="RH033_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV2992417.1"
                     /translation="MGKKTAPLLPYTNQMLSEFGERLKLARLRRRLTAKQVAERAGMS
                     PITLRSLESGSAGVTIGAYLSVMQVLGLETDLNKLAAEDDLGRQLQDSRLIKKRGLSR
                     ASSKNEEGSQTASLQMRHEKEGHLTTDLTALAEGSTPSITGETSTSLAALLKPIKKPL
                     KGN"
     gene            569..1836
                     /locus_tag="RH033_003271"
                     /pseudo
     CDS             569..1836
                     /locus_tag="RH033_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606689.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
     gene            complement(1974..3251)
                     /locus_tag="RH033_003272"
     CDS             complement(1974..3251)
                     /locus_tag="RH033_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2992418.1"
                     /translation="MNKIKNYRWYMIALVCFITVINYLDRTALGIAAPTIMETTGITK
                     EQYSWIVSAFQLAYTLGQPVMGFFIDTVGLKLSFAICAAIWGLATMGHALTGTWSGLA
                     FMRALMGFSEASAIPAGVKTASTWFPAKERGVATGVFNMGTSLGAMLAPPLIAWCIMF
                     HSWQFAFIVSGSLALLAALFWFFCYKDPKDAKRLSDEERHYIESGQEQHLKTDKKEKT
                     SIKHILSQRNFWGIGIARFLADPAWGTINFWVPIFFVETLHFSLKEIAMFVWLPFLLG
                     DLGCLASGFVAKFFHDRGVSLINSRRITFTIAAVIMMTIGLVSIVENPYIAVLLISIG
                     AFSHQCLSTVAATLGGDLFKKDEVATAVGMAGACAWSGQLIFNLFIGAFVHIIGFAPF
                     FIALAFFDIIGAIALWMLIKVKDEELQVQLATS"
     gene            complement(3344..5416)
                     /locus_tag="RH033_003273"
     CDS             complement(3344..5416)
                     /locus_tag="RH033_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDV2992419.1"
                     /translation="MSQLDEAILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWKTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFA
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPMLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQHGRQVFMDFNRNPEVVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLAAPA
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLSEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARMVIDPQGKCLP
                     QTRRGAKEEWRFRSELAEDKNHKLTIQYSQGSFITEVKSLRIQPCINGIYFEKNWPDF
                     LKGDIYTQ"
     gene            complement(5413..6954)
                     /locus_tag="RH033_003274"
     CDS             complement(5413..6954)
                     /locus_tag="RH033_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HDV2992420.1"
                     /translation="MRKLSTAETLAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDSKGTNRFAHAEMLKRIIAGHFTWSPKMQELVKSNAIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRKVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELAAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DALFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDRYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHIAQSGQIPKLVTQVEKITFSAAYAQQ
                     SSQEVLYITERAVFQLTAEGVELIEIAPGVEIDRDILPYMAFRPIIRHPRLMESSLFT
                     PMEDA"
     gene            complement(6964..7740)
                     /locus_tag="RH033_003275"
     CDS             complement(6964..7740)
                     /locus_tag="RH033_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDV2992421.1"
                     /translation="MSDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNEVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQVANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYSHQLLAGLMSATQDCQQATHAFAEKAGVSFHNQ"
     gene            complement(7750..8592)
                     /locus_tag="RH033_003276"
     CDS             complement(7750..8592)
                     /locus_tag="RH033_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDV2992422.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQIARQLAASNINVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPMGTKDLYEAREQVKQGIRQLLPHSKEVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            complement(8602..9393)
                     /locus_tag="RH033_003277"
     CDS             complement(8602..9393)
                     /locus_tag="RH033_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HDV2992423.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDRPDTDSVE
                     KLHATQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            9606..10253
                     /locus_tag="RH033_003278"
     CDS             9606..10253
                     /locus_tag="RH033_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDV2992424.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYISNP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKHNIFKPDIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            complement(10237..10875)
                     /gene="ytfB"
                     /locus_tag="RH033_003279"
     CDS             complement(10237..10875)
                     /gene="ytfB"
                     /locus_tag="RH033_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HDV2992425.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQSDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GADKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            11093..11713
                     /gene="fklB"
                     /locus_tag="RH033_003280"
     CDS             11093..11713
                     /gene="fklB"
                     /locus_tag="RH033_003280"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDV2992426.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            12022..13425
                     /gene="cycA"
                     /locus_tag="RH033_003281"
                     /gene_synonym="dagA"
     CDS             12022..13425
                     /gene="cycA"
                     /locus_tag="RH033_003281"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HDV2992427.1"
                     /translation="MVDQVKVVAEEQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPGLSDWVASLSVIILLLVLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLIMVMMHFQSPTGVEASFTHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VAEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVIVLLTLEDDTRQALIVTPLWF
                     ILLGLGWLFIGKKRMAK"
     gene            13560..13994
                     /locus_tag="RH033_003282"
                     /pseudo
     CDS             13560..13994
                     /locus_tag="RH033_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14093..15160
                     /locus_tag="RH033_003283"
     CDS             14093..15160
                     /locus_tag="RH033_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992428.1"
                     /translation="MAILHPQECWLLERIMSPEYYRRRFEGWQEFVELCERQVAEWSK
                     AMPLDVRRRPLCEQIDVVWGGRVLPNIRSTLKSVQYDFIQLQQGDLRVLQSGGNISSD
                     MKGLIDYPSDWMSLAAQKQYDRLKWRGAHYNNLIRRTSGGYWYDGELTYYYEESLHGP
                     LALPMQLPLYELDSSVYLREDDPVTVAGLYLPDIPDASAQLLYRSEHIPEAWQGRVRT
                     KYVNEAGIQEYYWESGAWAKCNWKRIRRVANRFINVPPEGFFPQGMPEELYNWPQREA
                     QYVTDRQRIAAFSGEACPHSGEWSIFVEGRQATVTLEQGEQMPEWTDRKMEGEYKRGE
                     KFHVLWSLMNRHDGGSVWVEA"
     gene            complement(15407..16069)
                     /gene="ytfE"
                     /locus_tag="RH033_003284"
     CDS             complement(15407..16069)
                     /gene="ytfE"
                     /locus_tag="RH033_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HDV2992429.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVDVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(16177..17142)
                     /locus_tag="RH033_003285"
     CDS             complement(16177..17142)
                     /locus_tag="RH033_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDV2992430.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPGFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVVGVVIAVRAKPEKPLTESVSES"
     gene            complement(17250..18110)
                     /gene="qorB"
                     /locus_tag="RH033_003286"
     CDS             complement(17250..18110)
                     /gene="qorB"
                     /locus_tag="RH033_003286"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HDV2992431.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADKSPLGLADEHIETEKMLADSSIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            18199..18579
                     /locus_tag="RH033_003287"
     CDS             18199..18579
                     /locus_tag="RH033_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDV2992432.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKMGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(18696..20639)
                     /gene="cpdB"
                     /locus_tag="RH033_003288"
     CDS             complement(18696..20639)
                     /gene="cpdB"
                     /locus_tag="RH033_003288"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HDV2992433.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMVNANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            20829..21569
                     /gene="cysQ"
                     /locus_tag="RH033_003289"
     CDS             20829..21569
                     /gene="cysQ"
                     /locus_tag="RH033_003289"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HDV2992434.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPIIGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
BASE COUNT         5348 a         5299 c         5515 g         5469 t
ORIGIN      
        1 ttgcaggtgc gttcaatcca ataaatgcta atattatggc tatcatagcc ataaaaagat
       61 aaaatattat cactatgggt aaaaaaacag caccgttact cccttatacg aatcagatgc
      121 ttagtgaatt tggtgagcgg ctgaaacttg ctcgtttacg acgcaggctt accgcaaagc
      181 aagttgctga acgtgctggg atgtcgccaa taacattgcg ctctctggag tcaggaagtg
      241 caggggtcac gataggggca tatttgtctg tgatgcaggt tttgggcctg gaaacggatt
      301 taaataagct tgcagctgaa gacgacttag ggcgtcagct ccaggattcc cgtttgatta
      361 aaaaaagagg gctttctcgt gcatcaagta agaatgaaga aggttctcag acagcctcat
      421 tgcagatgag gcacgagaaa gaaggtcatc tcaccacgga tttaacagca ctggcagaag
      481 gctcgacgcc atctattaca ggagaaacat caacctctct tgcagcacta ttaaaaccga
      541 taaaaaaacc gttaaaagga aattaattat ggcattaatc ggagtgtatg cggattggga
      601 aggcctggat ggtccggagc ggattggtta tttacacagt cgccgcactc ggacgcgtga
      661 gatatttgag tttgagtacg ataaaaaggc gcttgcagat ccttcactta atttcattca
      721 gttagatcct gaaataatgt tgtatgaggg agcacagtac ccaattcctc caaaggataa
      781 atttggtgca tttagtgatt cctgcccgga tcgttggggg cgtatgctaa tgaagcgtcg
      841 ttttgagagg gatattcgag acgggttatg tgataaagac tcccaccttt atgagtctga
      901 ttatctacta ggtgttcatg atctttatcg ggttggggct cttcgataca aacgtgaaga
      961 cgctggagag tttcttgata atcgcattga tgttgcagcc ccaccattta ccgaaatagc
     1021 gagtctggaa agggctagcc gggcaattga agaagatcct gataataaag agctaatggg
     1081 gcaaaaatgg ttaagaatgc taatcgctcc ggggggatca ttaggggggg cacgtccgaa
     1141 agccagtgtt gttgatgaag ctgggcatct ctacatcgct aaatttccca gtgtaaaaga
     1201 tgagtatgat gttggtggat gggaaatggt cgttaatgca cttgctgttg gctgtggact
     1261 gaatgttgca cctgctcagg cgcataaatt tgccagcaat taccattgtt ttatggttcg
     1321 tcgttttgac cgcactaatg cgggaaggcg tctgcatttt gcttcggcta tgacactaac
     1381 caggcatcag gatggtgaag atgcctctac aggtgtgagt tatctggaat tagcagatgt
     1441 tcttatcaga catggagcgc agactaatac tgaccttaag gaactctggt ccaggattgt
     1501 ttttaatatt cttgtttcta ataccgatga ccatttacgt aatcatggat atatcttgat
     1561 accgggaaaa ggttggcggc tttctgaggc atatgatttg aatcctgttg ccaggtctga
     1621 cggtctaaaa ctcaatatta cagaaatgat aatgcacttg atttggaatt agctcgagaa
     1681 gttgcggagt attttcgact tggtttgaca gaagcagatg acattattgc taattttaga
     1741 gggattgtca gtcagtggcg aatcattgcc gagcgtcttc gattacctgg tcgtgagcaa
     1801 gagttaatgg cagaggcatt caggggggca atttagaata gttattttga tatctaaatc
     1861 ataaatttgc gaagcaatgc cagtagatac tgaacgtttt ggaagagacg tttaatttat
     1921 gagaaaaata gaaacgctac aaagctaaat tatagcgttt ctatgtaaaa taattagctt
     1981 gtcgctaact gtacttgtag ttcttcgtct ttaactttga taagcatcca cagcgcaatg
     2041 gcgccaataa tgtcaaagaa agccagggca ataaagaacg gcgcgaagcc gataatgtga
     2101 acgaatgccc cgatgaacag gttgaaaatc aactgaccgc tccacgcaca ggctcctgcc
     2161 atacccactg cggtagctac ttcgtctttt ttgaacagat cgccacccag agttgctgct
     2221 acagtagaaa ggcattgatg cgagaacgcg ccaatactaa tcagtaatac ggcaatgtag
     2281 ggattttcga caatactcac caggccaatc gtcatcataa tgacggctgc aatagtgaag
     2341 gtaattcttc gtgagttaat taaactcacg ccgcgatcgt ggaagaactt cgcgacaaaa
     2401 ccactggcta aacagccgag atcgcccagc aggaaaggca gccagacgaa catggcaatt
     2461 tctttcaggc taaaatgcag cgtttcgacg aagaaaatcg gcacccagaa gttaatggtt
     2521 ccccatgccg ggtctgcgag aaaacgcgcg atgccaatcc cccagaaatt acgttggctg
     2581 aggatatgct tgattgacgt tttttctttc ttatctgttt taagatgctg ttcttgtcct
     2641 gattcaatat agtggcgctc ttcatcagaa aggcgtttgg catctttcgg atctttataa
     2701 caaaagaacc aaaataaagc cgcgagcaaa gcaaggctac ctgaaacaat aaacgcaaat
     2761 tgccagctat gaaacataat gcaccaggca atcaacggtg gagcaagcat cgcgccgagt
     2821 gaggtgccca tattgaaaac acctgtcgcc acgccacgct cttttgccgg gaaccatgtt
     2881 gatgcggttt ttacacccgc cggaattgcc gacgcttcgc tgaaacccat cagggcgcgc
     2941 ataaatgcca gaccagacca cgttccggtt agtgcatggc ccattgtcgc caggccccaa
     3001 attgcggcac atatcgcaaa acttaacttc agacccacgg tatcaataaa gaagcccatt
     3061 accggttgcc ctaatgtata ggccaactgg aatgcactga caatccatga atattgttct
     3121 ttggttattc cggttgtctc cataatcgtt ggagccgcaa tacctaacgc cgttctgtcc
     3181 agataattga ttacagtgat aaagcatacg agggcaatca tataccatcg ataattcttt
     3241 attttattca tttttaaacc ttttaattat attaataaac cctggttgtt ggtatttcca
     3301 tgtaataggc aataaaaatc aggtacagag gaaatattgg aaattattgt gtgtaaatgt
     3361 ctccctttaa aaagtctggc cagttttttt caaagtaaat accgttaata cacggttgaa
     3421 tacgtaaaga ctttacttcg gtaataaaag aaccttgcga atattgaatt gttaatttgt
     3481 gatttttgtc ttcagcgagt tcagagcgaa aacgccattc ttcttttgcg ccgcgacgag
     3541 tttgtggtag gcatttgcct tgtggatcta taaccatacg cgccccacgg ctaccaccac
     3601 cagcatcaat ataatgcgtt aattgtgtta ataccgcggc agaagtcagc gccatatgcc
     3661 gccacataaa cagctcggca acttcaccca catgtttgat tgccaatcca tgccgttgca
     3721 caaattcact caatagcagg gcatcacgag tggcgcgtcg gactttatcg gcatgacaaa
     3781 taaatcccgc atggtcagac attcgtgcct gaattttttc cctcacaacc gacaacggca
     3841 tcccggttcc gttatcgtgc gcctgagtaa tgatctctct tatagaagcc agtgccggag
     3901 ctgccagctg tgctatatct ccatcaatat tccgcttttg cgtgcaacca ataaaacgtg
     3961 ccagacgaac agcaaaaacc tgcccggcat tcaatgccgc accgccaggt cgggtgacgc
     4021 cgtgtgtgcc agcaacttcc cccacagcaa aacaaccggg cagagatgtt tgtccccaga
     4081 tatccacttc aatgccgcca ttcatatgct gattattcat ggcaaactgc aatggctgcg
     4141 tggtgagatc gtaaccgtgc atcttataca gcgagataga cagcggattc attcgttgca
     4201 gtcgttcgat gggcgatggt gccagagcgt cgttattttc cagatacgcg cgaacgtcgt
     4261 cgtccagtcg atctaatgag aagggcaggt cacccggcac cacttcagga ttgcgattga
     4321 aatccataaa cacctggcga ccatgttgct gctcttgcgc caccgccata tctaacaagc
     4381 tggagccaaa atccattacc cgcgtggcgt ggaacggcca ctgatagcct ttacggaaaa
     4441 tgtttgaagc cagctcttgg gtggtgcgat agtaatccgc gaggaagtta tactcgttgc
     4501 cctcggcatc cacggaatag atatacggga tcacctgcac ataggtgccg gacaaattcc
     4561 acggaaacgt gctgcgcggt gtgccgatac caaactggct ttcagtcaga ttggttagcg
     4621 tcagaccttc ctccagcgcc agccccagcg agccgaaaca tttgtgtgga tacacactgt
     4681 cgcgataaag ctcgccaggc cctcctgttg ccagtaccac attgggcgca gtcacaattg
     4741 ccagccccca tgggttatgg gcgcgatgac cggtcgcgag gattgccccc gccacacggt
     4801 cttcaccgtt ttcgtcacgc tgatgcagca gtttaatcac tgttgcactg gtcaacattg
     4861 ggatagcaag acgctgtact tcttccagca gcactttcac cattaacctt gaggtacgcg
     4921 gcccacacga ggttgcacgc ccggcttcgt catggtcggt ttgataacga agaatcgcgc
     4981 catagcgatc ttccggtaat tccagaccaa gatattgcaa cccgccaaga gtgtgcagag
     5041 aacctaccgc ttcgacataa gcggtgtcgt gatccatcgc cccaccgctc gccagtgcct
     5101 ctgccagttt ggcgaagttg tcgccgttgc ccgcggtagc ggcggtaaac agtgtttgtt
     5161 tatcagagcc agaacatgcc gatgtcccca tatataaccc ggcggtggcg attaacacat
     5221 tttgctggcg gcgtttaagt tccacagctg cacgtagccc ggctgcacca ctgccgacta
     5281 ccagggcatc tgttttccag agtgggtaat caacaccgtc taccgtgaca acccatgcgg
     5341 gttctgcctg tgcaaaaccc tttgggaaag taacgtgtgt cagtgcatca aggatggcct
     5401 catcaagctg actcatgcat cctccattgg cgtaaacagg ctactttcca tcaggcgtgg
     5461 gtgcctgata atcggacgga aggccatata cggcagaatg tcgcggtcaa tctctacgcc
     5521 aggggcgatc tcaattaatt caacgccttc tgccgttaac tggaataccg cacgttcggt
     5581 gatatacagc acttcctgac tgctttgctg tgcgtatgcg gcgctaaaag taattttttc
     5641 cacctgggta accagtttgg ggatttgacc tgactgggcg atatgcaggc catcaggcgt
     5701 tacatcaatc ttgctgcctt tagcgtcgaa cgtaccgcag aacaccactt tacgggcgtt
     5761 ttgcgcaatt tcgaggaagc cgccggggcc aatgagattg ccattgagat gggatacgtt
     5821 gacgttaccg taccgatcca tctctcccat gccgaggaag gtgatatcga taccgccgcc
     5881 actgtagaat tcgaattgat cgagtgatgg aataatggca tcggcgttac gggcgcagcc
     5941 gaacagagca tcatccagca tcatgccgtt atgtacaccc tgttctacac tcagccacaa
     6001 actttgataa ggcacgcctt cgcgcagtgc aaccccagga ataccgccgg gaataccaaa
     6061 gccaaagttg gttgaagcac ctgccgccag ttcccgcgct gcccgacggg caatcaggcg
     6121 acgcaccgga tgactaacca gttcaatagc gtcgttagaa cttagacggc gatgctgacc
     6181 gctgatggtc aggtcgtaac cgccaagata ggtttgcggt tgttcgcggt gttcaacgat
     6241 accatcgacc agaatccccg gtagcttgac cttgcgaggt tcaatggcgc cagcttccag
     6301 tacatcgcgc acctgtacaa acactttgcc gccactgttg tgtgctgcca gcgccatcga
     6361 atagctatcc atatcaatcg cttcttcttc gaggctaacg ttgccacgcg gatcggcata
     6421 ggtgccgcgc aaaatcgcgt agtccacttt gaaagggcga tagcggagtt tggtttcacc
     6481 gtcgatttca atcagttcta ccaggtcgtc ggtcgtgcat tcattcgact taccgccacc
     6541 attgcgtgga tcaacaaacg atcccagccc aacgtgggta aaaagccccg gacgtccggc
     6601 accgatctcc cgcagcagtg cctgaattac gccaccaggg aaacagtagg cttcaatagc
     6661 attgcttttc accagttcct gcatcttggg cgaccaggta aaatgtccgg caataatgcg
     6721 tttgagcatt tcggcgtgag caaaacggtt agtgcctttg ctgtcgcgat cgccaatacc
     6781 cagcgagtgg attaatgtca gatcgcgcgg gtgtccggtt tgcagaaagc gcgcttcaat
     6841 ggcggccaga atatggtcag cttccaccat gccgccgccg ttgccgctaa tggcaatggt
     6901 tgcgccgtcc tgaatttgcg ccgccagtgt ctcggcggtt gataacttac gcatacaatt
     6961 tccttattga ttgtggaagc tcacgccggc tttttcggca aatgcgtgcg tcgcctgttg
     7021 gcaatcctga gtggcggaca ttaatccagc cagtaactgg tgggagtaat cggcgtgttt
     7081 attttccagc gccagaatca gttgcttaat atgtgccagt gccaccggac cacatttttc
     7141 cagctgcgct aactggttcg ccatccagct ttccacctgt tctttttcta ccacttcatc
     7201 aattaaattc gccacctgag cttcctgcgc cgtcaggcgt ttacccagca tcagcatctg
     7261 gcgaccgacc accgggccaa cctgattaag cacgcgttca atgcccatcc agccaggcac
     7321 catgccgagc atcacttccg ggttagagaa tttcgcgccg gggcgagcga tacgaatatc
     7381 gcagcacaag gcaagttcaa taccgccacc gatggtatga ccgttgagat tcgcaacggt
     7441 taattggggt aagttgcgca ggcgattaaa aacctcgtta ccgcgtttaa tccattttct
     7501 gcccatatct aatggcgaat aggccgacca cgatttaata tcgccgcccg cacaaaagaa
     7561 tttatcgccg gttgccgtta attcgactaa tcgtaatgtg gtgtcgtttt caatttcatt
     7621 taaataatgg tcgagagatt cgatcatttc ttcattaata gcatgacatt tatcttcacg
     7681 gttcaatgtc aggcggcaag aagccgccgc tctgctgaac agaacgggtt ggtcactcat
     7741 gattattcct taacaataat gtgcgatgcg atcgacgcta atatccagcg tgctgttaat
     7801 atctttctgc caccagtacg gtgagaatat ttccagttca atagcgccat taaatccggc
     7861 gttttcaacc agccgacgaa ttgacggaat attgatgaca ccatctcccg gcattccacg
     7921 gtcattgacc agatcggtgg tcggcactaa ccagtcggaa acatgaaacg cgaggatgcg
     7981 ttttccggca cgcaggatct gactggcgag atccgggtcc caccagacat ggtagacatc
     8041 taccgccacg ccgaggccaa attcgccatc gggatcgagt tcatcacacc agtccagcgc
     8101 ctgacgtaac gtgcacaaac aggaacgatc ggctgcggtc atcgggtgta gcggctccag
     8161 cgcaatcggc acgccgacct ctttcgaatg tggcagtaac tggcgaatcc cttgtttcac
     8221 ttgttcccgc gcttcataca gatcttttgt tcccattggc agaccgccaa ccacctgcat
     8281 ataacaggcg gcgttcagta cagcggcgtc gtccagcgcc cggcggttat cgtcaatcgc
     8341 cagtttgcgc tccgccagcg tcggtgcggt gtagtaggtg ctacggcaca aaccggaaac
     8401 gtttatgtta ctggcagcaa gctggcgggc gatttgctgg agatcttcac cctgcaactc
     8461 cctgcgccag ggggcaatgg cgccaatacc gcgtgcggcg caggcatcaa taatggcggg
     8521 cagcggcgtt ttatagccca gtgtcgcggt attgatactg aataaatccg ggcgttgtaa
     8581 cagatctctc atgattcagc ctcagaagcg cggcatactt aagccgccgt cgatggcgac
     8641 cgcctgacca caggtgtaaa tcagtttacc ttccgccatc gcgcggacag tggaagcaat
     8701 atcagccgga taaccccaac gtccccacgg caccagacct ttggcaatca gttcgtcgta
     8761 ataggccgtt gccggaatag tcatatcggt tttgatgaga ccaggacgca cttcataaac
     8821 gccaatttgt tcattacaaa ggcgagcagc aaacagacga gcagcggcag agacggcagt
     8881 tttggcaatg gtatattcgc cgcgattcat cgccagcatg atggcgttga tggaactgac
     8941 gaaaataatc gagcggtgcg gcagttctgc ttccggtttc ggctgtgcca gtaagcgctt
     9001 actgaatgcc tgagcgagga agaacggtgc gcgggtgtta atggcaatat tttggtcgaa
     9061 gctgtctggt tcgaggtcga gaagatcacc gcgttttttc actgaaatgc ctgcgttgtt
     9121 gagcaggcag tccagacgtc cccactggtt ttgcgctgcg tcgagcatct cttcatgcag
     9181 ggagagatcg ccaacatctg cagggaagca aatcacttcc acgccttccg ccagacattc
     9241 ttgttgtgtg gcatgtaatt tttctacgct atcggtatcc ggacgatcat taatcagcag
     9301 attaaatccg ccacgcgcaa gctccagcgc acagcccttt ccaataccac gtgcggctcc
     9361 ggtaatcacg gcaacaggac gagtggattt catacaatag ctctcttttt ttgaattttg
     9421 tttccttagt gatgattagc ttgcgactgg aggataaaaa gatcaaccat ctagttggtt
     9481 gatatgcgat ggcgatcaca ggacatttga taacctgatg aaagtacaag tttttgccct
     9541 tttttctgaa gctgaaatca aatcaggtta tgatagtcac cacgaagagg tcgtggggaa
     9601 gcgttatgta tcttgatacc agcaacgtac agagcctgaa agaaaagctg ctgttatgcg
     9661 cggttaatga atttgccgaa tatggttacg aaggggccag agtcgataat atcgtcaagg
     9721 ccgcggggtg cagtaaacag acggtttatc accactttgg taataaagaa aatttattta
     9781 tcgaagtact ggaatatacc tggaacgata ttcgacaaaa agaaaaggcc cttgatttct
     9841 cagatcttcc gccacagaaa gccattgaga aaataatcga ttttacatgg gattattata
     9901 tttctaatcc ctggttttta aaaattgtgc atagtgaaaa tcaaagtaaa ggtgtgcatt
     9961 atgcaaaatc acagcgtctc ttagaaatta accatgcgca cttgcagtta atggaatcct
    10021 tattggatga agggaagaaa cacaatattt ttaagccaga tatcgaccca ttgcaggtga
    10081 atattaatat tgctgcgctt gggggatatt atttgatcaa ccagcatacg cttggcctgg
    10141 tttatcacat cagtatggtt tcaccccagg cactggaagc cagacgtaag gtcatcaaag
    10201 agacaatcct tagctggctt ttggttgacc cttcatctac cgcgcacgaa taaaactgcc
    10261 atccggctgg cgggtaaaca ggacctgttg attattcccc gtatcaatgg ttaagcccgt
    10321 caccacgccg ctggcgttct ggcggatttt caccatctgc ccgttttgca aattactcag
    10381 tggcttatcc gcgccttcta cctgcgccat ggcatagaca tcggtagcgg gcagtccgtg
    10441 gtcgcggaac agttgcgcca tcgttttacc aggttcgaca cgataggagc gccattgatt
    10501 atcaattccg ctatctgact ggaatggttg cgtttgggtg gtttgcggct gttcttctgg
    10561 ctggccttcc tgtattggtt ctggagcgac cggagccacc tgatcaggat cattttgtgg
    10621 cgtaactaat tgcgcccgca gctgttcttc tgtcggtggt tgtgattgcg actgaatatc
    10681 taactgtgct tcacgcgtca ccaccggcgc gttgggcgta tcatcagatg gaagcagaaa
    10741 tccgaccacc aatacaatgg cggcaataat gatcccccga cgatgcatcg gcggcagcgg
    10801 gtccatgaag cgaaaattgt ccggcgcgtg ccagactttc tccagggttg gttttagttc
    10861 aaagcgcccg ggcatggttt cctcctgctc cgcgtcttgt tcctcaatca tagcctatga
    10921 ataagctaac gctatgatgt ccgtggtaaa cccgccttta ttatattcat acgggatatt
    10981 gctattgttt ctttttcccc gggatttgtc atcattcccg cgacaaagtt ttacccaaag
    11041 aagtgtggct gatatgctgc ccgctacttt ataccctaag aaaggaaata cgatgaccac
    11101 cccaactttt gacaccatcg aagcgcaagc aagctacggc attggtttgc aggtagggca
    11161 acaactgagt gaatctggcc tggaagggct gctgccagaa gcactggttg caggcattgc
    11221 cgatgcgctg gaaggcaaac atccggctgt tccggttgat gtggtgcacc gcgcgctgcg
    11281 tgaaatccac gaacgcgccg atgccgttcg tcgtcagcgt ttccaggcga tggctgctga
    11341 aggtgtgaaa tacctggaag aaaacgccaa aaaagaaggt gtaaatagca ccgaatctgg
    11401 cctgcaattc cgcgtgatca accagggtga aggcgcaatt ccggcacgta ccgaccgcgt
    11461 tcgtgttcat tacaccggaa aactgatcga cggcaccgtg tttgacagct ccgttgctcg
    11521 cggcgagcca gctgaattcc cggttaatgg cgtgatccct ggctggattg aagcactgac
    11581 tctgatgccg gtaggttcta aatgggaact gactatcccg caggaactgg catatggcga
    11641 gcgtggcgca ggcgcgtcca tccctccgtt cagcaccctg gtgtttgaag tcgaactgct
    11701 ggaaatcctc taagcagcgc attctgttcc cctcagattt gaggggaatg gacaacagag
    11761 cgtaaaaact agaatttgct tactgtcgat attttatgtt gcaaatggct ctgttgcggt
    11821 tatttttgtg agctgtttcg cgttaccaga gtgatatgac cctcaaattc aacatgtaat
    11881 taacataata tttaaatctt agtattcatc ccgcttattc ttacctaata tcgatgagtc
    11941 ccgatacaga ttcgtcgtat catagacgta caaaaggccg cagagcctga acaacacaga
    12001 caggtacagg aagaaaaaaa catggtagat caggtaaaag tcgttgccga agaacaggct
    12061 ccggctgaac agtcgctacg gcgcaatctc acaaaccgac atatccagct tattgccatt
    12121 ggtggggcca ttggtacggg gctgtttatg ggttccggta aaacaatcag tcttgccgga
    12181 ccgtcgatca tcttcgttta tatgatcatc ggttttatgc tctttttcgt gatgcgagca
    12241 atgggggaat tgctgctttc gaatctggaa tacaaatctt ttagtgactt cgcttccgat
    12301 ctactggggc cgtgggcagg gtattttact ggttggacct actggttctg ttgggtagta
    12361 accgggatgg cagatgtcgt tgccattacc gcatatgcgc aattctggtt ccctgggctt
    12421 tctgactggg ttgcttcgtt atccgtgatc attctgttac tggttctaaa cctcgccacg
    12481 gtaaaaatgt tcggtgagat ggagttctgg tttgcgatga tcaaaattgt cgccatcgtg
    12541 tcactgattg tcgttggttt gatcatggtg atgatgcact tccagtcacc gactggcgtg
    12601 gaagcgtcat tcacgcattt gtggaatgat ggcggctggt tcccgaaagg tttaagtggc
    12661 ttctttgccg gattccagat agcggttttc gctttcgtgg ggattgagct ggtaggtaca
    12721 acagctgcgg aaaccaaaga tccggagaaa tcactgccac gcgcgattaa ctccattccg
    12781 atccgtatca ttatgttcta cgtcttcgcg ctgattgtga ttatgtctgt gacgccgtgg
    12841 agttcggtgg tcgcggagaa aagcccgttt gttgaactgt ttgtattggt aggtctgcct
    12901 gctgccgcaa gcgtgattaa ctttgtggtg ctgacgtctg cggcgtcttc cgctaacagt
    12961 ggcgtcttct ctaccagccg tatgctgttt ggtctggcgc aggaaggtgt ggcaccgaaa
    13021 gcgttcgcta aactctctaa gcgcgcagta cccgcgaaag ggctgacctt ctcctgcatc
    13081 tgtctgctcg gcggcgtggt gatgttgtat gtgaatccca gcgtgattgg tgcgttcacg
    13141 atgatcacga cggtttccgc gatcctgttt atgttcgtct ggacgattat cctttgctcg
    13201 tacctggtgt atcgcaaaca gcgtccgcac ctgcatgaga aatcaatcta caagatgccg
    13261 ctcggtaagc tgatgtgctg ggtatgtatg gcgttctttg tgttcgtgat cgtgctgctg
    13321 acactggaag atgacactcg ccaggcgctg atagttaccc cgttatggtt tatcttgctg
    13381 gggctgggct ggttgtttat tggtaaaaaa cgcatggcga agtaatgccg cgtttatcac
    13441 tctgaaaggc acctgcgggt gcctttgtct ttatacattc ctaaaaaaac ttaccgtcat
    13501 cgacatcccc ccaggcgcgg gcttaccgtg aatctttgcc actcattgca gagttcatta
    13561 tggttaagcg cagttggtta ttaatcgccg cattacctct ctccatttca ccttcctggg
    13621 gcgcggattt ttattaccgc cagcaggaga aaggcactgt ttacgttgcc gaacagaagg
    13681 ggaaaaaaga tgagatcctc tccgaactgc cggacgttaa tttgtcccgc ctctggcgca
    13741 ttgccaattt agccaataaa caagattccc ggttactgtc cgattttaat cccgataagt
    13801 tcgattgcga tgatgagggg gattgccagc atacctgact caccgatgga cgttctgtcc
    13861 tttgggcagg caaagtcctg aaaaatccat ccggtgaacc taatgttgat gccgccagtt
    13921 ttcaggcatt cggcgctttc gctgccgata aacgcagcgt ctattttgac ggtcagcgta
    13981 ccgatgatag atgaagtgga tatgtatata gctgtgtctc agttgcaaac aaatatttat
    14041 gtaccgaaat aaaagtatca atagacaatt aattaataaa tcaggagata aagtggctat
    14101 attacatcca caggagtgct ggttgctgga aagaataatg tcgccggaat attatcggcg
    14161 tcgttttgag gggtggcagg agtttgtcga gctgtgcgag cgacaggtgg cagagtggag
    14221 caaggccatg ccgctggatg tgcgccgtcg tccgctgtgc gagcagattg acgtcgtctg
    14281 gggcgggcga gtgttgccca atatccgcag tactttaaag tcggtacagt atgactttat
    14341 tcagctacag cagggggacc tgcgggtact gcaatccggg ggcaatatta gcagtgatat
    14401 gaaaggacta attgattatc cgtcggactg gatgtcactc gccgcgcaaa aacagtatga
    14461 cagactgaaa tggcgaggtg cacattataa taatttaatc agacgtacct cgggcggata
    14521 ctggtatgac ggcgaactga cttattatta tgaggaaagt ctgcacggcc cgctggccct
    14581 gccgatgcaa ttaccgttgt atgagctgga tagtagcgta tacctgcgtg aggatgaccc
    14641 ggtaacggtg gctggcctgt atctgccgga tatcccagat gccagcgccc aactgttgta
    14701 ccggagtgaa catattcccg aggcttggca ggggcgggta cgaacgaagt atgtcaacga
    14761 ggctgggata caagaatatt actgggaaag cggggcgtgg gcaaaatgca attggaaacg
    14821 tatacggcgg gtggcaaacc gatttatcaa tgtgccgccg gagggatttt tcccacaggg
    14881 catgccggag gagttgtaca actggccaca gcgcgaagcg cagtatgtta cagaccggca
    14941 gcggatagcg gcctttagcg gtgaagcttg tcctcatagc ggggagtggt cgatttttgt
    15001 agaaggtaga caagcgacgg tgacactgga gcagggagag cagatgccag aatggacgga
    15061 caggaaaatg gagggggagt ataaaagagg cgagaaattc catgtgctgt ggtcgctgat
    15121 gaaccgccat gatggcgggt cggtgtgggt ggaagcgtag cggtagctgg atgtacacgt
    15181 aaaaactagg gaatgggtga ttttattctg gcttatacag gagcgatgaa taagtaatta
    15241 tattaatgaa aaaaataagt tagtaaccgt tgaaggaact tattattggc tgaataatga
    15301 attttcccag ttcagtagat acaaaagatt gtcccggagt ggtgaataac tcaaagcatt
    15361 tgaaaacgca cctgattgtg taaaccaggt gcgttgatcg aacaaatcac tcacccgcca
    15421 gcgcgcgcgg gaacagtaca ttattttcca gactaatgtg atccatcagg tcatcaatca
    15481 attcattgat accgttatac atcgctttcc aggtggtgca ggcttctggc ggcggtgtga
    15541 cgttattggt ggtgtgtttg atcacttcca gcagttcacc cgcttcatcg tgctcgcttt
    15601 ccattacgct gattggcccc attgcctggc tgcccatgcc ttgtttgatc atcgggaaga
    15661 ggatctgctc ttctttcatc atgtggctgg aaagctcttc atgcagcata gtcaggtatt
    15721 ttgtcagccc ttttggcacg ctcggtttgt cggcgtgaac gcgctcgact ttagtcgctt
    15781 gcaggatcag ctccggcagc tgctcgcgat ggcgatcgtg gtagcgcacg atgatatggt
    15841 cgatgatttc tgccagcggg gcgctacgcc agtctttctc aatcggttgt tcagcaagct
    15901 ttgccagttc ggcttcaatg acgtcaacat ccagttcttt acgtgccgcc gcgcgcgcca
    15961 gcgtctgctt accgccacag cagtaatcca tatcatattt acgaaacaga gccgaagcgc
    16021 gaggaataga gagcgccagt tcacctaaag gttggtcgcg ataagccata gctgatacct
    16081 cattcttaat aagataagat gcattttaaa tgcatcttta aggcaaaagc tataaccctt
    16141 acgtagcaga ggcttattta actcactgcc agcgtgtcaa ctttctgaga cagattcagt
    16201 taaaggtttt tctggttttg ctctgactgc aatgaccacg ccaaccacta acagcgcgat
    16261 accgctcagc gtcattagcg gcggcatttg ctggcgtaac aaaaaggtgt acagcaagcc
    16321 tgccagcgtt tcgaaaacaa tcagcggccc gagaatcact gtcggtaatc gctggctggc
    16381 gacgttccag cagagtgcgc caacccatga gcaaagcacg gctatcgcaa ccatcagact
    16441 aataaacacc agcggacggg ggccaaaggg tagggagaag cccggcgttt gcgtattcag
    16501 ccagtaacag gcgacgagat agccgatgag agaaaccggc agcgtgacca gcgcctgcgc
    16561 cgtcgcccac atcatcggat gtttgtcggg attctcccgc agccagcggg cgttgcgcag
    16621 ggcataccat gcccagcaga ctacggaaac taacgccagc acgatgcctg aggtataacg
    16681 tgcccagtca aaatcgggga gtccgtggtt taactcagca atattcacac acgccaggcc
    16741 gatgccaata caaaccagtg ccggggcgag ttttccccat gcgagtttgc cgtcgcgctg
    16801 gctataaagc agattggcaa aaacgggaat caccaccggc agggtgccga taatcatcgt
    16861 ggaaacaggc gcgccagtac gttgaatggc acttgcaagg cagaagtaat agataaggtt
    16921 gcccatcatg gtgagcatca aggcggtaag ccagtcgcga cgcgccaact gacgcagacg
    16981 cacgcgtccc agccaggcaa tgggcagcgc aattaaccct agcgccaggt aacgccccat
    17041 cgactgcaac atcgccgggt attccggtac gatcaacggc ccgacaaaaa taagccccca
    17101 catcaaccct gctaacaggg cgtacagcac gccgctaatc attactggca tccattggtc
    17161 tgtcagaaga aagttcagga agcagacatg attgctcccg gcatcccgtc attatcagag
    17221 aggatgcggg aggatgccac tttaattaac tagttattaa cattaaaaag atggcttacg
    17281 ctttcggcta acgttgtcgt tgggcggcca atcaatttgc taagcgtttt gctgtcatca
    17341 aacagaccgc ctttcgatgc gccaacgtca gaatccgcca gcatatccgc cagtccatca
    17401 ggcagtccga cgcttttcag tgccgcagcg aaatcagctt cgctcaggtt ttgataggta
    17461 acctgtttgc cgctctgttt ggtgagttct gccgctaact gtgtcagcgt ccaggcacta
    17521 tcgcccgcca gttcgtaaac cttgccttcg tgaccggctt cgctaatcac gcgtgccgca
    17581 gctgccgcat aatctgcccg cgttgctgag gcgattttgc catcgcccgc cgcaccgata
    17641 aatacgccgt gttccagtgc tgccggggcg ctggcgaggt agttttcgct gtaccagccg
    17701 ttgcgcagca gggtgtaaac gatgctagaa tcagccaaca ttttctccgt ctcaatgtgc
    17761 tcatcggcga ggccgagcgg agatttatcc gcgtgaagca gactggtata agcgataaat
    17821 ttcacaccag ccgcctttgc ggcattaata acattacgat gctgcggggc acgttgaccc
    17881 acttcgctgg aagagatcag cagtagtttt tccactccct gaagtgcaga tgtcagtgcg
    17941 gcttcatcgc cgtagtcagc ctgacgcacg gtaatgcctt gtgctgtcag tgcctgggct
    18001 tttgccggat tacgaacgat agccactatt tggctggcag gaaccgtttt catcaaggat
    18061 tcaataacat agtgaccaag ttggccagtg gcaccagtaa ttgcgatcat aggaagtctc
    18121 catcgttttg tcttgtgttt aaatacgcta acacctaaac ttacttttag taagtacgta
    18181 caaaaaggta agtatgagat gagtcaggtt agcctgtcgc aacaactgaa agagggaaat
    18241 ctcttcgctg aacagtgtcc gtcgcgcgag gttttgaaac acgtcaccag ccgttggggg
    18301 gtgttgattc tggtggcgct acgcgaaggt actcatcgct ttagcgacct gcggcgcaaa
    18361 atgggcgggg tgagtgaaaa gatgcttgcg cagtcgttac aggcgctaga gcaggatggt
    18421 tttcttaacc gtatcgcgta tccggtggtg ccgccgcatg tggaatatag cctcacgccg
    18481 ctgggcgagc aggtgagcga aaaggttgct gcactggcgg actggattga gttgaatttg
    18541 cccgaggtgt tggcggtgcg ggatgaacgt gcggcgtaac tgcaggatgc gcggcctacg
    18601 gtgtatgact tgtaggacgg ataagatgcg ccagcatcgc atccgacaaa agtgtcggat
    18661 gcggttgatg atttgtaggc cgaaaaagaa gtgtattact tactcaaatc cacctgataa
    18721 atcgcaaacc cgatatcatc ggtcgcgact ttattcatcg gatattgccc tttctcttta
    18781 ataaacgccg cggctttatc tgatggagag gtttcgaaac ggatatccag tttcttatcg
    18841 ccagctattg gtgctaaacg ccagttgtta tctgccgccg ggtgaatttc ccccgcacgt
    18901 ttcgactcat cagcaatcca cgctgccagc accgagcggt tctcatccgg tgaagcaaaa
    18961 gcgatatggc tgtcgcccgt accggcaaat ttgccgccgt aagcgcgata gttattggtg
    19021 gcaacgagga acatggcgtt cggatcaatt ggcttgccat taaaggtcag gttcttaatc
    19081 ctttccgcat tggcattaac catctggcac tcgccgtcat aacgggcagg ctgggtaaca
    19141 tcaatctgat aattcacacc atcaatcaca tcaaagttat aggtgcggaa accatcccag
    19201 ttgatgagtg actgtggttt cgtgctgtta ggatcaatct ggttaaactg tccggcggag
    19261 cactccagcc actctttcac ctctttaccg ctggctttca ccacaatcag cgtattggga
    19321 tagagataaa gatcggcggc attacggaag gtcaactgac ctttttccac ctccacatag
    19381 cttgccgggt catttttgcg accaccaact ttaaacggtg cggcagctga aagcactggc
    19441 agttttgcca gatccggatc gccctgaatg taatgctcga catacgcttt ttgcgcgttg
    19501 ttcaccacct gcacggtcgg atcgtcctgc accagtgcca gatagctata catattgtcg
    19561 gcagatttac cgattggctt gctgacgaac tggcgtgtgg catcgtgatc ggctttgagt
    19621 gtttctacca gcttgctgtc ttccgccgcg agggattttt tattagcgat gtcgtaaatc
    19681 ggtcgtgctt ccgctttcgc ctgtgttacc tgccatttac cgctgtcatt actgagttgt
    19741 aagtcgacca caccaagatg atcgccccac atgcctggca ttaccgccgg aacaccattc
    19801 agcgtgcctt tcgcgatatc agctccttcg atatcagcaa aatctttacc tgggaaaacg
    19861 gcgtgagcat ggccaaacat aatggcgtta acgcccggaa tttcactgag gtaataaact
    19921 gagttttccg ccatcacttt atacggatcg gcagacagcc cggaatgtgc cagaacgaca
    19981 acgacatcgg cacctttctc gcgcatttca ggcacgtatt tgcgcacggt ttcggtaata
    20041 tcattcaccg tcactttccc ggataaatta gctttatccc agcccatgat ttgcggcggt
    20101 acgacgccaa tatagccaat cttcagcgtc tgtttttttc cgtctttatc gaccacttcg
    20161 gtgtctttaa ttaaatacgg tgtaaacatt ggctgtttgg ttctggcgtc aatgacgttg
    20221 gcatttacat aagggaattt cgctcccgcc agcgcatttt tcaggtaatc cagaccgtag
    20281 ttaaactcat ggttgccaag cgtgccgacg gtatagtcca gcgtatttaa tgccttatac
    20341 actgggtgaa tatcacctgc ttttaatcct ttcgccgaca tgtaatctgc cagcggactc
    20401 ccctgaatca aatcgccgtt atcgaccagt acgctgtttt tcacttcatt gcgggcatcg
    20461 ttaatcaggc ttgccgtacg taccagtccg aatttttccg tggcggtgtc tttgtaataa
    20521 tcgaaatcca tcatgttgct atgcagatca gtggtttcca tgatacgcag atcgaccgtc
    20581 gctgcattca cactggcggc aatcagcgtg gccaggagcg ttgcgctaaa cttaatcatc
    20641 agggacatcc ttttatcatc gggaatacga aagaaaaggg agaataaacg tcttacttat
    20701 agaacagtga agaatgccac aattttacgc tttgaaaatg atgacactat cacagttggc
    20761 gcattcatta acgatagggt ataagtaaaa caataagtta acaccgctca cagagacgag
    20821 gtggagaaat gttagatcaa gtatgccagc ttgcacggaa tgcaggcgat gccattatgc
    20881 aggtctacga cgggacgaaa ccgatggacg tcgtcagcaa agcggacaat tctccggtaa
    20941 cggcagcgga tattgccgca cacaccgtta tcatggacgg tttacgtacg ctgacaccgg
    21001 atattccggt cctttctgaa gaagatcctc ccggttggga agtccgtcag cactggcagc
    21061 gttactggct ggtagacccg ctggatggta ctaaagagtt tattaaacgc aatggcgaat
    21121 tcaccgttaa cattgcgctc atcgaccacg gcaaaccgat tataggcgtg gtgtatgcgc
    21181 cggtaatgaa cgtaatgtac agcgcggcag aaggcaaagc gtggaaagaa gagtgcggcg
    21241 tgcgcaagca gattcaggtc cgtgatgcgc gcccgccact ggtcgttatt agccgatccc
    21301 atgcagatgc ggagctgaaa gagtatctgc aacagcttgg cgaacatcag accacgtcca
    21361 tcggctcttc gctgaaattc tgccttgtgg cggaagggca ggcgcagctg tacccgcgct
    21421 tcggaccaac gaatatttgg gacaccgccg ctggacatgc tgtggccgca gctgccggag
    21481 cgcacgttca cgactggcag ggtaaaccgc tggattacac tccgcgcgag tcgttcctga
    21541 atccggggtt cagagtgtct atttactaaa ttcagatggc agaaacagtg tatttcctga
    21601 ttctgccatc ctgatttctc ccaacctaaa a
//
LOCUS       DAPHCV010000087        21243 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.087, whole genome shotgun sequence.
ACCESSION   DAPHCV010000087 DAPHCV010000000
VERSION     DAPHCV010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.087"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(373..1569)
                     /gene="hemY"
                     /locus_tag="RH033_003290"
     CDS             complement(373..1569)
                     /gene="hemY"
                     /locus_tag="RH033_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HDV2992435.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1572..2765)
                     /gene="hemX"
                     /locus_tag="RH033_003291"
     CDS             complement(1572..2765)
                     /gene="hemX"
                     /locus_tag="RH033_003291"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDV2992436.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAVQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPTPQADAPAA
                     APQGE"
     gene            complement(2787..3527)
                     /gene="hemD"
                     /locus_tag="RH033_003292"
     CDS             complement(2787..3527)
                     /gene="hemD"
                     /locus_tag="RH033_003292"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HDV2992437.1"
                     /translation="MTILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDSLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3524..4465)
                     /gene="hemC"
                     /locus_tag="RH033_003293"
     CDS             complement(3524..4465)
                     /gene="hemC"
                     /locus_tag="RH033_003293"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDV2992438.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSLIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4852..7398
                     /gene="cyaA"
                     /locus_tag="RH033_003294"
     CDS             4852..7398
                     /gene="cyaA"
                     /locus_tag="RH033_003294"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HDV2992439.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRREVLTQLVKEWEWDDARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7438..7758)
                     /gene="cyaY"
                     /locus_tag="RH033_003295"
     CDS             complement(7438..7758)
                     /gene="cyaY"
                     /locus_tag="RH033_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HDV2992440.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7806..9863)
                     /locus_tag="RH033_003296"
     CDS             complement(7806..9863)
                     /locus_tag="RH033_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992441.1"
                     /translation="MINTSRQDGFMHFRNVVCLLSCSLFLSSAWGCRLSAPEHNIYQK
                     QGKGVVYLRPYKETNLSLPDVNYKSLRRLPNLLIDPTTLDKWDKVPPLTDLTTDYLYE
                     GAQAWYPHYSWHSDGRYILYAGEVVQNPPDKPPVDVASFKAWGDFAADKHSLYFEGTR
                     TDDNGGENSLDIKTLHQVKFRSPWKPDFLGLILRDANFLYVDGHRLADPESFRVLAQK
                     SWDQRGKFSTTFNPCVAVPFGPWDTLARTRTKIIINGEQLKADPETFTVVRWMPGSLL
                     TWRDKSGLHRKVLNQGNLDKDRANSCAAFTLQEHSVSWRKGPECQQEVLPGLDPEQFH
                     PLSNTVAQYQDKLYTIKKTGFGENRLNIVTLDNPGLVIDKRFNAGRTHGYLLTKLPAD
                     GEEEDGLQVFDSSGPLILMDYRVPDEHEAHLSDSPHYKKWYAHDDRYVYAFDGSQLWR
                     YPTPNTKAVRVKWKTKDSGYGYWANYRSGELDGRLLEDGAFIPTGIPYNSSVNIAQYS
                     DGEGSFFVGETDVSWRKLRSTDQQWSQWEKLPDIDPKAFHPITDRVAQYKNHLYIAKL
                     SPFGEDTLEIITLDSSAPFLKQRINAGKGHGYFMRSSRLRDDIQIFAIDGPLKTTERF
                     AYDNRYVYTWIGSQLYRTASPCPARTRNLDQNMFSSNDDIIILTTEEECHKTPDIEQT
                     LKL"
     gene            complement(10205..10759)
                     /locus_tag="RH033_003297"
     CDS             complement(10205..10759)
                     /locus_tag="RH033_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HDV2992442.1"
                     /translation="MGLFTQLEKFDQKLTRGYARWGRWVWRTLIVVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSITCCGDVSGDTAEVIWT
                     LSTTRTQYNAGMRLEKRNMTLPLPKREWGENYLHVHFMPSDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKLYGTGKMN"
     gene            11101..11304
                     /locus_tag="RH033_003298"
     CDS             11101..11304
                     /locus_tag="RH033_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV2992443.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVEM
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            11341..12165
                     /gene="dapF"
                     /locus_tag="RH033_003299"
     CDS             11341..12165
                     /gene="dapF"
                     /locus_tag="RH033_003299"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDV2992444.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            12162..12869
                     /locus_tag="RH033_003300"
     CDS             12162..12869
                     /locus_tag="RH033_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HDV2992445.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDCWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            12866..13762
                     /gene="xerC"
                     /locus_tag="RH033_003301"
     CDS             12866..13762
                     /gene="xerC"
                     /locus_tag="RH033_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDV2992446.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            13762..14478
                     /gene="yigB"
                     /locus_tag="RH033_003302"
     CDS             13762..14478
                     /gene="yigB"
                     /locus_tag="RH033_003302"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HDV2992447.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            14562..16724
                     /gene="uvrD"
                     /locus_tag="RH033_003303"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             14562..16724
                     /gene="uvrD"
                     /locus_tag="RH033_003303"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HDV2992448.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(16769..17671)
                     /locus_tag="RH033_003304"
     CDS             complement(16769..17671)
                     /locus_tag="RH033_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HDV2992449.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAQTLGIDSVREIDEKANRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LKQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(17754..18518)
                     /locus_tag="RH033_003305"
     CDS             complement(17754..18518)
                     /locus_tag="RH033_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HDV2992450.1"
                     /translation="MAHRLFIGKGMITLNLKRIFLTLTLLPLFAVAADDCAPSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     LLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVG
                     QLLYLDGTISHMYMKGEAIPWQRYPFVTMISVEQKG"
     gene            18888..19838
                     /gene="corA"
                     /locus_tag="RH033_003306"
     CDS             18888..19838
                     /gene="corA"
                     /locus_tag="RH033_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HDV2992451.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(19874..20371)
                     /locus_tag="RH033_003307"
     CDS             complement(19874..20371)
                     /locus_tag="RH033_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HDV2992452.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHFFIADNNEYQLTQENKIERVFNPHDFEK
                     DNFQQ"
     gene            complement(20368..21216)
                     /locus_tag="RH033_003308"
     CDS             complement(20368..21216)
                     /locus_tag="RH033_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HDV2992453.1"
                     /translation="MNNILGSSINMPCTLFETITLFDDFSADDMQYGDMEEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPNSTYGAFSVPTSGTKISQSECIDILFAEMKDLAKMFS
                     FFGQYKTLIQDLIDHFRYGNGNSFHSQELNLSFHERINKYDYNSPIRVIKECIENDIS
                     STPTIGYRPLLLQKIKTELLSSRLNKFNDFEDSFNGLGISVHDISAQKISLLSFQKYA
                     IGWSATIHFVAQDHFGLDVTDIKNKTYSKYRFFRIWFFLQRHKDFAFKPFFTNFNTIE
                     RIENYL"
BASE COUNT         5054 a         5394 c         5439 g         5356 t
ORIGIN      
        1 cgcaggagaa atattcactc aggaagttat tactcaggaa gcaaagagga ttacagaatt
       61 atctcataac aagtgttaag ggatgttatt tcccggttct ctgtggcata ataaacgagt
      121 agatgctcat tccatctctt atgttcgcct tagtgcctca taaactccgg aatgacgcag
      181 agccgtttac ggtgcttatc gtccactgac agatgtcgct tatgcctcat cagacaccat
      241 ggacacaacg ttgagtgaag cacccacttg ttgtcataca gacctgtttt aacgcctgct
      301 ccgtaataag agcaggcgtt tttttatgta tcagaaaggt cccggaggag cttgcctccg
      361 ggtgagaaag agctactgtg gcgggttgtt ctgcaacgtt aacatcaaac cgtcgcgacg
      421 catcgctgcg gcttcttccg gcttgtgcag tctgtccagc gcgtcggcaa gccatgcgta
      481 atcgtaggcg tccggacgtt gtttcagcgc tgcgcggaag gcgagcgatg cttcctgcca
      541 ttctccgtgt ttcatcagcg actgacccag tgtgctccac aacagcgggc gatcgcccac
      601 gtttttgatc tgttggcgca gcactttttc cagctgttcc gggttattgg ttttcagacg
      661 cgggatcggc agcagcaggc gatcgtcata ctggcgtttc aggccatcga tgataatttg
      721 ctgggcagta tcatgatcgt cacactcaat aagatgttcc gccattgcca cctgcaaggc
      781 cacctgatga cgcgttttcc ggctttggtt tttccaccag ttacgcaaac cttcactacc
      841 gttatcggca cgcgcctgat ccatcaggcc aatccatgcc tgttgttcca gcattgcacg
      901 atgttcttca tcaccaacat gggctttcgc cattgatggg atgatatcca gcagcgaact
      961 ccatgcacca gtgcggatat acgcctgttc cgccagacgt aatacttccg gatggcgtgg
     1021 cgtaacttcc agcagcttat ccacgccgtg acgtgctgca tggttttcat tacgggccag
     1081 ttgcagacgt acacgggtga tttctaccgg aatggtgtca ttgccggcca gctccgctgc
     1141 gcgttccaga tgttggttgg cgcgtgcttc atcaccacgt tgttgcgcgg cttcggctgc
     1201 cagtagatag ttcaccaccg gttgttcagc gtgatcggca tttttcgcca tcagcttttc
     1261 aacttgctga taatcgcctt ccgccagttt cagcagcgcc tgttcggtct gcttacgtgc
     1321 acggcgacgc ttacgtccga caaaccaccc acgggtgtgc gcgcctgtgc ggaagattcg
     1381 ccgcagtagc cactcaatgg caaacagcac taccatcgcc agaatcaaaa taatcgcgag
     1441 gcccgtgacg ctagtttcaa tattgtagtt gtcggtctgg atcagcacgt aaccctgatg
     1501 gccggcaatc atcgggccaa ccacgatccc cgcaatcagc aacacaaaga gcaataacac
     1561 ttttagcatg gttattctcc ttgcggcgcg gctgccggag catcagcttg cggtgtaggt
     1621 gcaggtgcag gtgcaggttt agcttccgtt gtccctgctg ccggttgtgc cagcaggtta
     1681 cgcacgcggg tctgcatcaa tttttccagc atcgcctggc tttgcagggt ttccggaaga
     1741 tccatcgaga tattttgctg gcttaactgg tccacctcgt cgaggaacgc tttggtggtg
     1801 gcatcatcag tatcgtagta agcacgtacc caggtggaga cgttctccag cgcctggcga
     1861 taagtctctt cctggtgacg cggtacagct tgtgctgcga ccagcaggcg agagcgaata
     1921 ttttcgcgca gatagatatc ctgatttggc gctaacagcg gtacggcggt gtcatcacga
     1981 cggcgaatcg taatgaagtt gtccataaag ttctgccagc ttttttgcag attgatacgc
     2041 cattcgctga tggaactgga aagctcttca ccgtcggaat ccatcggcga accatcgctg
     2101 tcattatcag ccagacgcag attatctacc tgatttgaaa gctgattaag cttaaggatg
     2161 ataccgtcat aatccacctg cgataccgca gaaaggctgg cgatatcatc ggtaattgcc
     2221 cgacgaacgg taatcagact cgggtcattc atatccgcca ggctggcatc tgcacttttc
     2281 agcaacgctg cagcggtcgt gacgtcttga tcgctccaca gcttccgtcc tgcgagtttc
     2341 accagaaaat cggcctgagc cagcagccag gttttagcat cgctgccgga aatggtggcg
     2401 accttttgtt ggacttcatc caactgtttt gccagcgttt cttgctgacg attcgcctgc
     2461 tcaagctgta cagcttgttg cttgataatg ccttccagct cggctttttg gctctcctgg
     2521 gctttttgca atgccgtcag ttggttagcc agggcatcgc tggtggcggt ctgattgacg
     2581 gcctgttgtt taccccagcc atacaaaccg acgcccgccg ccagagcaat agcgatagcc
     2641 accgcgctga gaatcaatgc ggtattgttc ttactctttt tttctgttgc gacaggttgt
     2701 gacgtggtgt ccacggcctc cctggtctct tcaaccacgg cggaggtttt ttcttgttcc
     2761 gtcattatgg cttcctgtta tgagagttat tgtaatgccc gtaaaagcgc atcgttgtca
     2821 gcgttatcgg cgaccttaat gtcttgccag cccagttccc gggcgagttt cgccaaacgc
     2881 tcactgacga ccaatagtcg acagcgtagt aaccagtgct cacgatacca ttgtgggatc
     2941 agcgaccaga gttgctgcaa catttcaccg ctggtaacaa cgaccgtcgt cacctcgcgg
     3001 gattgccagc gcatcgcttc ttctgcacca tcgtaatgga ttgcgcatcg ttgataacat
     3061 tcacaaaaag tgacctcagc accgcgcgcc gtcagggaat ccccaattag ctcacgaccg
     3121 ccattgccac gtaatatcag cgcacgtttg cccgcaatat tttgtaattc aggtaattgt
     3181 agcaagactt cgctgatttc ccgatcctgc gggtagagaa tcttctgtcc gcttacggta
     3241 tgtagtgcca gtgcggtggt gcgtccaatg gcgaaataat caggtagtcg gggccattta
     3301 cgatcttgct gatgcagctg tgattgggca aaagcaaccg cgtgttgcga gagggcaaac
     3361 aacagatcgc tctctcccag cgctgccagt tgatcagcaa gttgcggtaa ttgtcgaccc
     3421 ggagaaaact caatcagcgg aaaatgccag gccacctgcc ccagtgtgcg cagacggctc
     3481 actaactctt ctcctgcggg agacgggcgg gtgactagga tagtcatgcc ggggcgtctc
     3541 cgttatagac ttcagcgagg atctcgcgcg cgccgttatt cagtagctct tctgccagcg
     3601 aaatccccat ttgttcggca tcttgcggcg caccgcggcg ttcaccgcga ataatcagcg
     3661 aaccgtccgg cgcgccgacc aacgcacgca gccagatttc gccatcaata agctcggcgt
     3721 agctaccaat tggtacctga catccgcctt cgagacgggt attcatggcg cgttctgcgg
     3781 taacgcgcag tgcagtttcg tggtgattca gcgcggcaag cagctcgcga gtgcgtgtat
     3841 catcaaggcg gcattcaatg cccaccgcac cttgtcctac cgccggaaga gaaatctcgg
     3901 gtggcaacgc ggcgcgaatg cgtgactcca gacctaaacg ttttagtccg gctacggcaa
     3961 gaatgatggc atcgtattcg ccgttatcca gtttgctcag gcgagtgccg acgttgccgc
     4021 gcagggagcg gataatcaga tccgggcggc gttcagccag ttggcactgg cgacgtaaac
     4081 tggacgtccc gacgatactg cctgccggta acgcatccag actgtcatag ttattggaca
     4141 caaaggcatc gcgaggatct tcacgctcac aaatagtgac cagtcccaga ccttgcggga
     4201 attcaaccgg cacatctttc attgaatgta cggcgatatc ggcgcgattt tcgaggagcg
     4261 cgacttccag ctcttttaca aataagcctt ttccgcctac tttcgccagc ggcgtatcaa
     4321 gaatcacatc gccgcgcgtc accatcggta ccagttcaac gaccaggccc ggatggctcg
     4381 ccatcaactt gtctttgaca tagtgtgcct gccagagtgc aagtgggctt tggcgtgtgg
     4441 caattcttaa aacattgtct aacatgcttg ttaccgtcat tatcatccgt ggtccatcct
     4501 aacatccttg ccagagcgat gtcagtgttg tggtgaaacg tagacgcctg cgcaaaccgt
     4561 aaaatgaggt ctggcagtgg atcctgacag gcgtttcacg ccgtcgtaat aaggaattta
     4621 cagagaataa acggtgctac acttgtatgt agcgcatctt tctttacggt caatcagcaa
     4681 ggtgttaaat tgatcacgtt ttagaccatt ttttcgtcgt gaaactaaaa aaaccaggcg
     4741 cgaaaagtgg taacggttac ctttgacata cgaaatatcc cgaatgccgc gtgttaccgt
     4801 tgatgttggc ggaatcacag tcatgacggg tagcaaatca ggcgatacgt cttgtacctc
     4861 tatattgaga ctctgaaaca gagactggat gccataaatc aattgcgtgt ggatcgcgcg
     4921 cttgctgcta tggggcctgc attccaacag gtctacagtc tactgccgac attgttgcac
     4981 tatcaccatc cgctaatgcc gggttacctt gatggtaacg ttcccaaagg catttgcctt
     5041 tacacgcctg atgaaactca acgccactac ctgaacgagc ttgaactgta tcgtggaatg
     5101 tcagtacagg acccaccgaa aggtgagctt ccaattactg gtgtatacac catgggcagc
     5161 acctcgtccg tagggcaaag ttgttcctct gacctggata tctgggtctg tcatcaatcc
     5221 tggctcgata gcgaagagcg ccagttgcta caacgtaaat gtagtctgct ggaaagctgg
     5281 gccgcctcgc tgggtgtgga agtcagcttc ttcctgattg atgaaaaccg tttccgtcat
     5341 aatgaaagcg gcagcctggg gggcgaagat tgtggctcca cccagcatat actgctgctt
     5401 gacgaatttt atcgtaccgc cgtgcgtctc gccggtaagc gtattctgtg gaatatggtg
     5461 ccgtgcgacg aagaagagca ttacgacgac tatgtgatga cgctttacgc gcagggcgtg
     5521 ctgacgccaa atgaatggct ggatcttggt ggcttaagct cactttctgc tgaagagtac
     5581 tttggtgcca gcctttggca gctctacaag agtatcgatt ccccgtacaa agcggtactg
     5641 aaaacactgc tgctggaagc ctattcctgg gaatacccga acccacgact gctggcgaaa
     5701 gatatcaaac agcgtttgca cgatggcgag attgtatcgt ttggcctcga tccatactgc
     5761 atgatgctgg agcgtgttac ggaatacctg acggcgattg aagatttcac ccgtctggat
     5821 ttagtacgcc gctgcttcta tttaaaagtg tgcgaaaaac tcagccgcga acgcgcctgc
     5881 gtaggctggc gtcgtgaagt gctaacccag ttagtgaaag agtgggaatg ggacgatgcg
     5941 cgtctggcaa tgctcgataa ccgcgctaac tggaagattg atcaggtgcg tgaggcgcac
     6001 aacgagctgc tcgatgcaat gatgcagagc taccgtaatt tgatccgctt tgcgcgtcgc
     6061 aataacctta gcgtctccgc cagtccgcag gatatcggcg tactgacacg taagctgtac
     6121 gccgcgtttg aagcattgcc aggtaaagtg acgctggtaa acccgcagat ttcgcccgat
     6181 ctctcggaac cgaatctgac ctttatttat gtgccgccgg gccgggctaa ccgttcaggt
     6241 tggtatctgt ataaccgcgc gccaaatatt gagtcgatca tcagccatca gccgctggaa
     6301 tataaccgtt acctgaataa actggtggcg tgggcatggt ttaacggcct gctgacctcg
     6361 cgcacccgct tgtatattaa aggcaacggc attgtcgatt tgcctaagtt gcaggagatg
     6421 gtcgccgacg tgtcgcacca tttcccgctg cgcctgcctg caccgacgcc aaaagcgctc
     6481 tacagcccat gtgagattcg ccatctggcg attatcgtta acctggaata tgacccgaca
     6541 gctgcgttcc gcaatcaggt ggtgcatttt gatttccgta agctggacgt cttcagcttt
     6601 ggcgagaatc aaaattgcct ggtgggtagc gttgacctgc tgtatcgcaa ctcgtggaac
     6661 gaagtgcgta cgctgcactt caacggtgag cagtcgatga ttgaagccct gaaaaccatt
     6721 ctcggtaaaa tgcatcagga cgccgcaccg ccagatagcg tggaagtctt ctgttatagc
     6781 cagcatctgc gcgggttaat ccgtactcgc gtgcagcaac tggtttctga gtgtattgaa
     6841 ctgcgtcttt ccagcacccg ccaggaaacc ggacgtttca aggcgctgcg cgtttctggt
     6901 caaacctggg gcttattctt cgaacgcctg aatgtatcgg tacagaaact agaaaatgcc
     6961 atcgagtttt atggcgctat ttcgcataac aaactgcacg gcctgtcagt gcaggttgaa
     7021 accaatcacg tcaaattacc ggcggtggtg gacggctttg ccagcgaagg gatcatccag
     7081 ttcttcttcg aagaaacgca agacgagaat ggctttaata tctacattct cgacgaaagc
     7141 aaccgggttg aggtgtatca ccactgcgaa ggcagcaaag aggagctggt gcgtgacgtc
     7201 agtcgcttct actcgtcatc gcacgaccgt tttacctacg gctcaagctt catcaacttc
     7261 aacctgccgc agttctatca gattgtgaag gttgatggtc gtgaacaggt gattccgttc
     7321 cgcaccaaat cgattggcaa catgccgcct gccaatcagg atcacgacac gccgctatta
     7381 cagcagtatt tttcgtgatg agcgtgccgg aaagcgaggc ttatccggca tgcaatctta
     7441 gcggaaactg actgtttcac ccgcttgctg cgttgccgcc tgttccagca aatcccagaa
     7501 ggtttcgccg ctacgatcgc aaatccactc atcgcctttc agatcaaaat ggtagccgcc
     7561 ctgtttggtt gccagccata cctggtgcag cggttcctgg cggttgataa tgattttgct
     7621 cccgttttca aaggtaatgg tcagtacacc gccgttgatt tcgcagtcga tatcgctatc
     7681 gccatcccag tcgtccagac gctcttcaat ggtcagccac agttgatcag ccaggcgatg
     7741 aaattcactg tcgttcattg ttgtatcctg tttttaagtg atggcggcag tatagcggca
     7801 tggggtcaga gcttcaaagt ttgctcaatg tctggtgttt tgtggcactc ttcctccgtc
     7861 gtcaagatga tgatgtcatc attggatgaa aacatgttct gatccagatt acgtgtcctg
     7921 gccggacagg gcgatgcggt tcgataaagt tggctaccga tccaggtata aacatagcga
     7981 ttgtcataag cgaaacgttc ggtggttttc aatggtccat caatagcaaa aatttgaata
     8041 tcatcgcgta aacgggaaga gcgcataaag taaccatggc ctttcccggc gttaatccgt
     8101 tgtttcagga aaggtgcaga agagtcgagc gttattattt ccagggtgtc ttcaccaaaa
     8161 ggagacaatt tcgcaatgta cagatgattt ttgtactgcg cgacacgatc ggtgatggga
     8221 tgaaatgctt tcgggtcaat atcggggagt ttctcccatt gactccattg ctggtctgta
     8281 gagcgaagct tacgccagct aacatcggtt tcgccgacga aaaaagagcc ttcgccatca
     8341 gaatattgag caatgttaac ggatgagtta taggggattc ccgttggaat aaacgcacca
     8401 tcctcaagaa gtctgccgtc aagctcacca ctacggtagt ttgcccaata gccatatcct
     8461 gaatctttgg ttttccattt cacccgcacg gctttggtat tgggagttgg gtagcgccaa
     8521 agctgtgagc catcgaatgc gtaaacataa cgatcgtcgt gtgcatacca tttcttgtaa
     8581 tgggggctat cgctaaggtg agcctcatgc tcgtcaggaa cacgataatc catcaggata
     8641 agcggcccgg aagaatcgaa tacctggagg ccatcttctt cttcaccatc agctggtaat
     8701 ttggtcaata aatagccgtg agttcttccc gcgttaaagc gtttatctat caccaaacca
     8761 ggattatcca gtgtgacgat atttagccta ttttcgccaa atcccgtttt tttgatggtg
     8821 tacagcttgt cctgatactg agcaacggta ttactaagag gatgaaattg ttctggatcg
     8881 aggccgggta ggacttcttg ctggcattct ggccctttgc gccatgaaac agagtgttcc
     8941 tgcaaagtga acgccgcgca gctatttgcc ctatctttgt ccagatttcc ctgattcagc
     9001 accttccggt gcaggccgct tttatctcgc caggttaata acgaaccggg catccagcgt
     9061 acgacagtaa atgtctctgg gtcggcttta agctgttcgc cattgattat gattttggtc
     9121 cgtgtacggg ctaacgtatc ccaggggcca aatggtacgg caacgcaggg gttaaacgtg
     9181 gtggaaaact ttcctcgctg atcccaggac ttctgcgcca gcacacggaa actctcgggg
     9241 tcagcgaggc gatgtccatc gacatataag aaattagcgt cacgtaatat cagccctaga
     9301 aagtcaggtt tccaggggga acggaatttc acctgatgca gcgttttaat atctagacta
     9361 ttttctccgc cattgtcatc ggtgcgcgtg ccttcaaaat agagactgtg tttatctgcg
     9421 gcaaaatcac cccatgcctt gaacgaggcg acatcgacgg gaggtttgtc tggcgggttt
     9481 tgcacaactt cacccgcgta gaggatatag cgaccatcgc tatgccagga gtaatgagga
     9541 taccaggcct gtgcgccttc atagaggtag tcggttgtca gatcagtcag aggaggtact
     9601 ttatcccatt tgtctagtgt tgtcgggtct attaataaat tgggtaaacg gcgcaaactt
     9661 ttgtaattca cgtcaggaag agaaagattt gtttctttat aagggcgcag gtataccacg
     9721 ccttttccct gtttctggta aatattatgt tcaggcgcgg aaagccgaca tccccatgcc
     9781 gacgataaaa acaacgaaca agacaacaaa caaaccacat tgcgaaagtg cataaagcca
     9841 tcctggcgcg aggtgttgat cacgaaactg ctagcagaac ataaatcctc acaagtaagc
     9901 gtaactctcg cagcaaatcg tcacttttca gaatccataa aggtcatcgc tattttctga
     9961 tagttgctgg aataaacgtg atattttcat tcacgatata aacacaatcc cggaactgcc
    10021 ccatagtacg gctaatagca taaaaatatt ccggattgac ccctggaagg gaggcaatgt
    10081 gggattccgc cccccgttgc cttctttatt gtctttcacg agttgtaccc aggttccacc
    10141 acgggtatct aacattcgcc acattattac gctttctgcc gaacgtgatt tttatagaaa
    10201 tctatcaatt cattttccct gtaccataaa gtttatcctt cacatcctgc cgaacgcggg
    10261 tcttgtaccc cccgttgtga agatcttcat acggagaaaa agaatcttta ctccacccca
    10321 gcaacacttt atcgctaggc ataaaatgca cgtgcaggta attttctccc cattctcttt
    10381 ttgggagagg taacgtcata ttgcgtttct ccagacgcat tccagcatta tactgtgtac
    10441 gagtagtact taatgtccat ataacctctg ccgtatcccc actgacatca ccgcaacagg
    10501 tgattgaacc gccaccattc gcaaaagcat tcgcgcccgc cacgccgtta acactgaatc
    10561 ccagtatcgg acggtcaatt tcactgtgga tttccagtat cactccacct cttggtcctc
    10621 cccatactgc ctgtcctata ttccacaaca cgaccagtac tggaacgaca attaacgtcc
    10681 gccataccca gcgcccccag cgggcataac cgcgcgtaag cttttggtca aatttttcca
    10741 gttgggtaaa tagccccatc gattttattc cctcttctca cctggttggc aaagttgcac
    10801 gtcactggcg actaaggtca gacaacgaaa tttcgtggta cgccactctc ttaaaagtcc
    10861 catgcaggcg ataagaacaa gacaacaaac aaaccacatt gcgatagtgc ataaagccat
    10921 cctggcgcga ggtgccgatc acgaaactac cagcaaaaca taaatcgcca cgtgtaagcg
    10981 ttatactcgc agcatttcct cacttttcag acttcataaa gagtcgctaa acgcttgctt
    11041 ttacgccttc tcctgcgatg atagaaagca gaaagcgatg aactttacag gcaatccata
    11101 atgaaaaacg tgtttaaggc actcactgta ttacttactc tcttcagcct gacgggctgc
    11161 ggtctgaaag gtccgctcta tttcccgcct gcagataaaa acgcaccgcc gccgaccaaa
    11221 ccggtagaga tgcaaacgca atccacggtg ccggataaaa acgaccgcgc cactggcgat
    11281 ggtccatccc aggtgaatta ctaaaagtca gtttctgtac ccgcgtgatt ggagtaaatg
    11341 atgcagttct cgaaaatgca tggccttggc aacgatttta tggtcgtcga cgcggtaacg
    11401 cagaatgtct ttttttcacc ggagctgatt cgtcgcctgg cggatcggca cctgggggta
    11461 gggtttgacc agctgctggt ggttgagccg ccgtatgatc ctgaactgga ttttcactat
    11521 cgcattttca atgctgatgg cagtgaagtg gcgcagtgcg gcaacggtgc gcgctgcttt
    11581 gcccgttttg tgcgcctgaa agggctgact aataagcgcg atatccgcgt cagcaccgcc
    11641 aacgggcgga tggttctgac cgtcaccgat gatgatctgg tccgcgtaaa tatgggcgaa
    11701 cccaacttcg aaccttccgc cgtgccgttt cgcgctaaca aagcggaaaa gacctatatt
    11761 atgcgcgccg ccgagcagac aatcttatgc ggcgtggtgt cgatgggaaa tccgcattgc
    11821 gtgattcagg tcgatgatgt cgataccgcg gcggtagaaa cgcttggtcc tgttctggaa
    11881 agccacgaac gtttcccgga gcgcgccaat atcggtttta tgcaagtggt taagcgcgag
    11941 catattcgtt tacgcgttta tgagcgtggg gcaggagaaa cccaggcctg cggcagcggc
    12001 gcgtgtgcgg cggttgcagt agggattcag caaggtttgc tggccgaaga agtacgcgtg
    12061 gaactccccg gcggtcgtct tgatatcgcc tggaaaggtc cgggtcaccc gttatatatg
    12121 actggcccgg cggtacatgt ctacgacgga tttattcatc tatgaagcaa ccaggggaag
    12181 aactgcagga aacactcacg gagcttgatg accgggcggt tgtcgattat ctgattaaaa
    12241 atcctgagtt ttttatccgc aatgcgcgcg cagtagaagc gatacgtgtg ccgcatccgg
    12301 tacgcggcac cgtttcgttg gtcgagtggc acatggcccg cgcacgtaat cacattcatg
    12361 ttctggaaga gaacatggcg ttgttgatgg agcaggctat cgccaacgaa ggcctgtttt
    12421 atcgcttact ctacttgcag cgtagtctca ccgccgccag cagtctcgac gatatgctga
    12481 tgcgctttca ccgctgggcg cgcgatctcg gcctggcggg tgcgagcctg cgcctgttcc
    12541 cggattgctg gcgtttaggt gcgccgtcga accacactca tctggcatta agccgtcagt
    12601 ctttcgaacc gctgcgtatt cagcgtttgg ggcaggaaca gcactatctt gggccgctta
    12661 acggaccgga gctgctggtg gtgctaccgg aagcgaaagc ggtgggatcg gtggcgatgt
    12721 cgatgctggg aagcgatgct gatttgggtg tcgtgctgtt taccagtcgc gatgccagtc
    12781 actatcaaca agggcaggga acgcagttac ttcatgaaat tgcgctgatg ttgccggagc
    12841 ttctggagcg ttggattgaa cgcgtatgac cgatttacac accgatgtag aacgctacct
    12901 acgttatctg agcgtggagc gtcagcttag cccgataacc ctacttaact atcagcgtca
    12961 gcttgaggcg atcatcaatt ttgccagtga aaacggcctg caaagctggc agcaatgtga
    13021 tgtgacgatg gtgcgcaatt tcgctgtacg cagccgccgt aaagggctgg gagctgcaag
    13081 tctggcgtta cggctttctg cgctacgtag cttttttgac tggctggtca gccagaacga
    13141 actcaaagcc aacccggcga aaggtgtttc ggcaccgaaa gcgccgcgtc atctgccgaa
    13201 aaacatcgac gtcgatgata tgaatcggct gctggatatt gatatcaacg atcccctcgc
    13261 cgtgcgcgat cgggcaatgc tggaagtgat gtacggcgcg ggtctgcgtc tttccgaact
    13321 ggtggggctc gatatcaaac acctcgacct ggagtccggc gaagtgtggg tgatggggaa
    13381 aggcagcaaa gagcgccgcc tgccgattgg tcgcaacgct gtggcgtgga ttgagcactg
    13441 gcttgatttg cgcgacctgt ttggtagcga agacgacgcg ctttttctgt cgaaactggg
    13501 caagcgtatc tccgcgcgta atgtgcagaa acgctttgcc gaatggggca tcaaacaagg
    13561 gctgaataat cacgttcacc cgcataaatt acgtcactcg ttcgctacgc atatgctgga
    13621 gtcgagcggc gatcttcgtg gtgtgcagga gctgctgggt catgccaacc tctccaccac
    13681 gcaaatctat actcatcttg attttcaaca ccttgcctcg gtgtacgatg cggcgcatcc
    13741 acgcgccaaa cgggggaaat aatgcgtttt taccggcctt tggggcgcat ctcggcgctc
    13801 acctttgacc tggatgatac cctttacgat aaccgtccgg tgattttgcg caccgagcga
    13861 gaggcgctta cctttgtgca aaattatcat ccggcgctgc gcagcttcca gaatgaagat
    13921 ctgcaacgcc tgcgccaggc ggtacgggaa gcggaacccg agatttatca cgacgtgacg
    13981 cgctggcgtt ttcgttcgat tgaacaagcc atgctcgacg ccgggctgag tgccgaagaa
    14041 gccagtgcag gcgcacacgc agcaatgatc aactttgcca aatggcgcag ccgaatcgat
    14101 gtcccgcagc aaacccacga caccttaaaa cagctggcga agaaatggcc gctggtggcg
    14161 atcaccaacg gtaacgccca gccggagctg tttggtttag gggattattt tgagtttgtg
    14221 ttgcgcgctg gcccgcacgg gcgctcaaaa ccgttcagcg atatgtactt cctggcggcg
    14281 gaaaaactca acgtgccgat cggcgagatc ttacatgtcg gggacgatct caccactgac
    14341 gtgggtgggg caattcgcag cggaatgcag gcctgttgga tcagaccgga aaatggcgat
    14401 ctgatgcaaa cctgggacag ccgtttactc ccgcatctgg aaatttcccg gttggcatct
    14461 ctgacctcgc tgatataatc agcaaatctg tatatatacc cagctttttg gcggagggcg
    14521 ttgcgcttct ccgcccaacc tatttttacg cggcggtgcc aatggacgtt tcttacctgc
    14581 tcgacagcct taatgacaaa cagcgcgaag cggtggccgc gccacgcagc aaccttctgg
    14641 tgctggcggg cgcgggcagt ggtaagacgc gcgtactggt gcatcgtatc gcctggctga
    14701 tgagcgtgga aaactgctcg ccatactcga ttatggcggt aacgtttacc aacaaagcgg
    14761 cggcggagat gcgtcatcgt atcgggcagc tgatgggcac cagccagggc ggcatgtggg
    14821 tcggcacctt ccacgggctg gcgcaccgtc tgctgcgtgc gcaccatatg gacgccaatc
    14881 tgccgcagga ttttcagatc ctcgacagcg aggaccagct acgcctgctc aaacgcctaa
    14941 tcaaggcgat gaacctcgac gagaagcagt ggccgccgcg ccaggcaatg tggtacatca
    15001 acagccagaa agatgaaggc ctgcgtccac atcatattca aagctacggt aatccggtgg
    15061 agcagacctg gcagaaggtg tatcaggcgt atcaggaagc gtgtgaccgc gcgggcctgg
    15121 tggacttcgc cgagctgctg ctacgtgctc acgagttgtg gcttaacaag ccgcatatcc
    15181 ttcagcacta tcgcgaacgg tttaccaata tcctggtgga tgaattccag gataccaaca
    15241 acattcagta tgcgtggatc cgcctgctgg cgggcgatac cggcaaagtg atgatcgttg
    15301 gcgatgacga ccagtcaatc tacggctggc gtggggcgca ggtcgagaat attcagcgtt
    15361 tcctcaatga tttccccggt gctgaaacta tccgtctgga gcagaactac cgctctacca
    15421 gcaatattct gagcgccgct aacgccctga ttgaaaacaa taacgggcgt ctgggtaaaa
    15481 aactgtggac cgatggcgcg gacggtgagc ctatttccct ctattgcgct tttaacgaac
    15541 tcgacgaagc gcgttttgtg gttaaccgta tcaagacctg gcaggacaat ggcggggcgc
    15601 ttgccgagtg cgccattctc taccgcagca acgcccagtc gcgtgtactg gaagaagcac
    15661 tgttgcaggc gagtatgccg taccgtattt acggcgggat gcgcttcttc gaacgccagg
    15721 aaatcaaaga tgcgctctcg tatctgcgcc tgattgccaa ccgtaatgac gacgcggcgt
    15781 ttgagcgcgt ggtgaataca ccaacgcggg gtattggtga ccggacgctg gacgtggtac
    15841 gtcagacatc gcgcgatcgc cagttaacac tctggcaggc atgtcgtgaa ctgttgcagg
    15901 aaaaagccct cgccggacgt gctgccagtg ccttacagcg gtttatggaa ctgatcgacg
    15961 ccttagcgca ggaaactgcc gatatgccgc tgcatgtaca gactgaccgg gtaattaaag
    16021 actccggcct gcgcacaatg tacgagcagg agaaaggcga aaaaggccag acgcgtatcg
    16081 aaaacttaga ggaactggta acggcgacgc gccagttcag ctacaacgaa gaagacgaag
    16141 atttaatgcc gctgcaggcg ttcctctccc atgcggcact ggaagcgggc gaggggcagg
    16201 cggatacctg gcaggatgcg gtgcagttga tgacgctaca ctcggcgaaa ggtctggaat
    16261 tcccgcaggt gtttatcgtt ggtatggaag agggcatgtt cccaagccag atgtcgctgg
    16321 atgaaggcgg gcgtctggaa gaagaacgcc gtctggccta cgttggcgta acccgcgcga
    16381 tgcagaaact gacgctgacc tacgcggaaa cccgccgtct atatggcaaa gaggtttacc
    16441 atcgcccgtc gcgctttatc ggtgagttgc cggaagagtg tgtggaagag gtgcgcctgc
    16501 gcgccacggt aagccgcccg gtcagccatc aacggatggg gacgccgatg gtcgagaacg
    16561 acagcggcta caagctcggc cagcgcgtac gccacgctaa gtttggtgaa ggcaccatcg
    16621 tcaatatgga aggcagcggt gagcatagcc gtttgcaggt ggcgttccag ggccagggta
    16681 ttaaatggct ggtggcggct tacgcccggt tggagacggt gtgacgttgc gggatgcggt
    16741 gcgccacacc gcattccgca tcagcacctt acttagcgca cttcttcaca tcaatattcc
    16801 agtcagcacc agacgcaacc tgatgtttct gcgcaaaatt atccatattc agcgccacgg
    16861 aaacattcaa taagctgttc agatacacca tcggctggcc ttccggcaca tcgccgaagc
    16921 ttgcgacata cggcattttc cctgcgtatt tttgctggga accttcggaa atggtcacac
    16981 acaacgtgtc gttgagtttg attcctgcct gtttcagcaa ctcgtcgctg atattgctcc
    17041 agacgttgcc gtactgaata tcgagaatcg ggatattgcc cttcacttca ccttttgttg
    17101 cttttgcttt ctgataagaa agttcgacaa cttttgcagg aagctctggc cccacctgtt
    17161 cgaacgtaat cgctccggaa gccagacgcg caccagtgta ggcatataca tcgcgaccat
    17221 ggaaggtata tgacttttca gaacctttca ggcggttagc tttttcgtcg atttcccgga
    17281 cgctatcaat acccagagtt tgtgccacca aagtgagcgt accgttatct ggtgaaacga
    17341 agtattgacc gtttttggtt ttcagcacta ccgatttacg gtcagtgcct acgcccggat
    17401 cgaccacgct aacaaacacc gaaccttgcg gccagtaact ggcagtctga tacaggcgat
    17461 aagcgccttc ccagatgtta tacggtggaa tttcgtgtgt taaatcaaag attttaagat
    17521 tatggtcaac gccgaatgcg acccctttca tcgccgagac ggcaccatct ttcaggctaa
    17581 aatccgtttg taaaatcaaa gcgttatccg cataagcgga tgatgacccc agacaggtta
    17641 ataaaaataa tgttgcgagc gtttttttca tttttgtttc ccttcattta tctgatgatt
    17701 ttccagctgt gtagccagcc ccagtttgtc tggcctcctc gagtgtacaa tgtttaacct
    17761 ttctgctcga cggaaatcat ggtgacaaaa ggatagcgtt gccacgggat tgcctcgcct
    17821 ttcatataca tatgtgaaat tgtgccatca agataaagca gctgcccaac gttcagtttc
    17881 gctttggcat aacaggcaaa atcataaaaa tttgtcgcct gctggctcaa caaaaacacg
    17941 gcgttcccat gtttattaat cccaacaccg ttacgaattt tacgtgaggc gacgttggga
    18001 tgaatacgcg gattaattac accgttttcc atcaacaatg gccctgactg caccgcaaac
    18061 tgaatctctt tactggtttt aaaggcatcc agacgaacga tgccgacttt atctcccgcg
    18121 acataaaaca ctccgccagg acgaataaag aaattccctt cacctgaagc gagatttaac
    18181 gccaccttct gctgaccgtt ttcgatgtac aaaccgagcg gcgcatagct ttcatcatag
    18241 atgccgccgt tcatcgccat ctgcacctga ccctgactat taatatccgc cagcagagca
    18301 tgtaacgttc cccacgcttc gccattggct ttttgccagt acattttcac ccgctctgtt
    18361 tgaggattaa cggtatacgc ctgtacggtc agcgtcggat ctgagggtgc gcaatcatca
    18421 gcggcaacag caaacaaggg aagtaaggtg agggtgagaa aaatccgttt gagattcaag
    18481 gtgatcattc ctttaccaat gaatagccga tgcgccatta taggccctgg atgtgggatt
    18541 tttttatcct gttagcgacc ttgacgagta ccaaaaagcg cgaagttcaa ctattgttct
    18601 gtggtgttct gttgcgtgtt gacggcaaaa ttttgctggc gtaacatgcg cgcacgatca
    18661 ctctaagagg acattcgcct tggacacacc cagtagatac tggctcacta tcctgtcatc
    18721 caggatcaac tcctaaggct atcccttttt gctgatagcc ttagcggttg tcagcgacct
    18781 caatttttcc cgtcgcgctg agtcaggctg tttaatggtc tgaaacccaa tttgtttctg
    18841 tgtgcccacc gaactgtccg atattttaag cattgggagt cccggtcatg ctgagcgcat
    18901 ttcaactgga aaataaccga ctgacccggc tggaagtcga agagtcacaa ccccttgtaa
    18961 atgcagtatg gattgatctt gtcgaaccgg acgacgacga gcgactgcgc gtacaatctg
    19021 aacttggtca gagcctggca acccgcccgg aactggaaga catcgaagca tcggcacgtt
    19081 tctttgaaga cgatgacggc ctgcatattc actccttctt cttctttgaa gatgcggaag
    19141 atcacgccgg taactctact gtggcattta ccatccgtga tggtcgtctg tttactctgc
    19201 gtgagcgtga actgcctgca ttccgtctgt atcgtatgcg cgcccgtagc cagtcaatgg
    19261 tggacggtaa cgcctacgag ctgctgttgg atctgttcga aaccaaaatc gaacagttgg
    19321 cggatgaaat tgaaaacatc tacagcgatc tggagcagtt gagccgggtg attatggaag
    19381 ggcatcaggg cgatgagtac gacgaggcgc tctccacgct ggcggaactg gaagatatcg
    19441 gctggaaagt tcgcctgtgt ctgatggata cccagcgtgc gctcaacttc ctggtacgta
    19501 aagcacgctt accgggtggg caactggagc aggcgcgtga aatcctgcga gatatcgaat
    19561 ccctgctgcc gcataacgaa tccctgttcc agaaggtgaa cttcctgatg caggcggcga
    19621 tgggctttat caatatcgag cagaaccgca tcatcaaaat cttctcggtg gtatccgtgg
    19681 tgttcctgcc accgacgctg gtcgcttcca gctacgggat gaactttgag tttatgccgg
    19741 aactgaagtg gagcttcggc tacccaggcg cgattatctt tatgatcctc gcgggcctgg
    19801 caccgtatct gtactttaag cggaagaact ggttgtaata taaatgccgg gtaaagattg
    19861 ttctttatcc ggcttattgc tgaaaattgt ctttttcaaa gtcatgcggg ttgaacactc
    19921 gctctatctt attttcctgg gttagttgat actcattgtt atcagcaata aaaaaatgag
    19981 tattacggta aggatattca ttaataacca ttaaatagtt ttttacgatg caattatcat
    20041 tgctcttaat cgtcgggaaa cagtagagat cttcatacgg cccttcgata tattttttat
    20101 agtcagtgaa gccattaccg atatcaataa cataccaggt atgccaacta ccttctggta
    20161 ggggatgttg atttttaatt tcattctgat gcttcagata ccaatttatt ttatcgctgt
    20221 cagtccatgg cagatgatca acgaccagaa agactgaatt gccagcatca gagtatgtat
    20281 acaaaatctg tacaggacgc aatgtccata aattatagat gcagcataga agaaataaac
    20341 ataatataat tttctggagt ttgcttttca tagataattt tcgattcttt ctatagtgtt
    20401 aaaattggtg aaaaaaggtt tgaaagcaaa gtctttatgc cgttgtaaaa agaaccatat
    20461 gcgaaaaaat ctatatttgc tataagtctt gtttttgata tcagttacat caagaccgaa
    20521 gtggtcctgt gctacaaaat gaattgttgc actccatcct atagcgtatt tttgaaaact
    20581 taaaagagat attttttgag ctgaaatatc atgaacactg attcctaagc cattaaaact
    20641 gtcctcgaag tcattaaatt tatttagtct tgatgaaagt aattcggtct taatcttttg
    20701 taggagaaga ggccggtatc ctatcgtagg tgttgaagaa atatcatttt cgatacattc
    20761 tttgattaca cgtattgggc tattataatc atatttgttt attcgttcat gaaaagataa
    20821 gttcaattcc tggctatgga aactgttgcc atttccatat ctaaaatgat caatcagatc
    20881 ctggatgagt gttttgtatt gtccaaaaaa ggaaaacatt tttgccagat ctttcatttc
    20941 tgcaaataaa atatcaatac attcactttg tgatattttt gttccagaag ttggaacact
    21001 aaaagcacca taagtagaat tcggcccagg aaaatggtat ttaatgaggc ggtaaggatc
    21061 aacttttgca gagatgtcgc tcaagccgag tgagagaaaa tcttgttctt ccatatcccc
    21121 atattgcata tcatcagcag aaaaatcatc gaataaagta atagtttcga aaagtgtaca
    21181 gggcatatta attgatgagc ctaatatgtt gttcatttat attctccttt cttataatta
    21241 atg
//
LOCUS       DAPHCV010000088        21237 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.088, whole genome shotgun sequence.
ACCESSION   DAPHCV010000088 DAPHCV010000000
VERSION     DAPHCV010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.088"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            123..1820
                     /gene="ypdA"
                     /locus_tag="RH033_003309"
     CDS             123..1820
                     /gene="ypdA"
                     /locus_tag="RH033_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HDV2992454.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            1835..2569
                     /gene="ypdB"
                     /locus_tag="RH033_003310"
     CDS             1835..2569
                     /gene="ypdB"
                     /locus_tag="RH033_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HDV2992455.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            2582..3439
                     /locus_tag="RH033_003311"
     CDS             2582..3439
                     /locus_tag="RH033_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV2992456.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPVNNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPSGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(3442..5937)
                     /gene="ptsP"
                     /locus_tag="RH033_003312"
     CDS             complement(3442..5937)
                     /gene="ptsP"
                     /locus_tag="RH033_003312"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDV2992457.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTLAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRVIPASAQDSTLLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRHLMAEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDKKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSVPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGELQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQAFSNKEQAIQFLCGNLGVNGRTERPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLVKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(5962..6999)
                     /gene="ypdE"
                     /locus_tag="RH033_003313"
     CDS             complement(5962..6999)
                     /gene="ypdE"
                     /locus_tag="RH033_003313"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDV2992458.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQSVRITTR
                     EECKIPGLLDGDRQGNDVSTMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(6999..8084)
                     /gene="ypdF"
                     /locus_tag="RH033_003314"
     CDS             complement(6999..8084)
                     /gene="ypdF"
                     /locus_tag="RH033_003314"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDV2992459.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVLISR
                     ESAHILVDSRYYADVEARTQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWQFELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     CEIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARRV
                     ITEAGYGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(8099..9346)
                     /locus_tag="RH033_003315"
     CDS             complement(8099..9346)
                     /locus_tag="RH033_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDV2992460.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKTSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPADIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFSAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(9368..9694)
                     /locus_tag="RH033_003316"
     CDS             complement(9368..9694)
                     /locus_tag="RH033_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HDV2992461.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9913..10878)
                     /gene="glk"
                     /locus_tag="RH033_003317"
     CDS             complement(9913..10878)
                     /gene="glk"
                     /locus_tag="RH033_003317"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HDV2992462.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEGIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            11082..12338
                     /locus_tag="RH033_003318"
     CDS             11082..12338
                     /locus_tag="RH033_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HDV2992463.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWLR
                     LPGTLGIAQDSPFWIIAILTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     QMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVMGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNI"
     gene            12453..12779
                     /locus_tag="RH033_003319"
     CDS             12453..12779
                     /locus_tag="RH033_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HDV2992464.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12920..14158)
                     /locus_tag="RH033_003320"
     CDS             complement(12920..14158)
                     /locus_tag="RH033_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDV2992465.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKMMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            14494..15696
                     /gene="nupC"
                     /locus_tag="RH033_003321"
     CDS             14494..15696
                     /gene="nupC"
                     /locus_tag="RH033_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HDV2992466.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPVAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(15745..17934)
                     /locus_tag="RH033_003322"
     CDS             complement(15745..17934)
                     /locus_tag="RH033_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HDV2992467.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLSKEKRAFTLS
                     WLAALSVLLLLMCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVLTEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(18142..18217)
                     /locus_tag="RH033_003323"
     tRNA            complement(18142..18217)
                     /locus_tag="RH033_003323"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18182..18184),aa:Ala,seq:ggc)
     gene            complement(18257..18332)
                     /locus_tag="RH033_003324"
     tRNA            complement(18257..18332)
                     /locus_tag="RH033_003324"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18297..18299),aa:Ala,seq:ggc)
     gene            18553..18912
                     /locus_tag="RH033_003325"
     CDS             18553..18912
                     /locus_tag="RH033_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HDV2992468.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPESQGETPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18914..19306
                     /locus_tag="RH033_003326"
     CDS             18914..19306
                     /locus_tag="RH033_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HDV2992469.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRHFLARLDIDESE"
     gene            complement(19357..20772)
                     /gene="gltX"
                     /locus_tag="RH033_003327"
     CDS             complement(19357..20772)
                     /gene="gltX"
                     /locus_tag="RH033_003327"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDV2992470.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLTAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            21031..21106
                     /locus_tag="RH033_003328"
     tRNA            21031..21106
                     /locus_tag="RH033_003328"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21064..21066,aa:Val,seq:tac)
     gene            21151..21226
                     /locus_tag="RH033_003329"
     tRNA            21151..21226
                     /locus_tag="RH033_003329"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21184..21186,aa:Val,seq:tac)
BASE COUNT         5244 a         5679 c         5272 g         5042 t
ORIGIN      
        1 ctaatacacc atttatctat caatcccctt ttaaaattgt ggtttttcct caaaaacggc
       61 ctgctggtca tcagccttca ggttacctat catagaggtt taatccttat tcagagtcac
      121 ccgtgcacga aatattcaac atgctgctgg cggtcttcga tcgggcagcg ttaatgctta
      181 tctgcctgtt ctttctcatc cgtatccgtc tgtttcgcga actgttgcac aagtcggcgc
      241 actccccaaa agagttgctc gccgttaccg ccattttctc gctgttcgcc ctgttcagca
      301 cctggtccgg cgttcctgta gaaggttcgc tggtgaacgt acgtattatc gcggtgatgt
      361 caggcgggat tctgtttggc ccgtgggtgg gcatcattac cggcgtgatt gcgggtattc
      421 accgttattt aattgatatc ggtggcgtga cggcgatccc ctgctttatc accagcattc
      481 tggcgggttg tatatcgggc tggatcaacc tgaaaatccc caaagcacag cgctggcgcg
      541 tcggtattct cggcggcatg ttgtgtgaaa cgttgaccat gattctggtg attgtctggg
      601 caccaactac cgcattgggg atcgatatcg tctctaaaat cgggattcca atgatcctcg
      661 gcagcgtctg tatcggcttt attgtgttgc tggtacaaag tgttgagggc gaaaaagagg
      721 ccagcgccgc gcggcaggcc aagctggcgc tggatatcgc caacaaaacg ctgccgctgt
      781 ttcgccatgt caatagcgag tcattacgca aggtctgcga aattatccgc gatgacattc
      841 acgctgacgc ggtggcaatc accaataccg atcatgtgct ggcctacgtt ggcgtgggtg
      901 aacataacta tcagaatggc gatgacttca ttagcccgac tacccgccag gcgatgaatt
      961 atggaaaaat catcattaaa aacaatgatg aagcccaccg cacaccagag attcattcca
     1021 tgctggtgat cccattgtgg gagaaaggcg ttgtgaccgg aacgctgaaa atttactact
     1081 gccacgcgca tcagataacc tcgtcattac aggaaatggc ggtcggtctg tcacaaatca
     1141 tctccacgca actggaagtt tcgcgcgctg agcagctgcg tgaaatggca aataaggcag
     1201 agcttcgcgc cctgcaaagc aaaattaatc cccatttcct gtttaacgct ttgaacgcta
     1261 tttcatcgtc aatccgtctg aatccggata ccgctcgcca gctgatcttt aatctgtcgc
     1321 gttatctgcg ctataacatt gaattaaaag acgatgagca aatcgatatc aaaaaagagc
     1381 tgtatcaaat taaagactat attgccattg agcaggcccg ctttggcgac aagctgacgg
     1441 ttatctatga tattgatgaa gaggtgaatt gctgcattcc cagcctgctg atccagccgt
     1501 tggtggaaaa cgccattgtc cacggtatcc agccttgcaa aggtaaaggc gttgtcacca
     1561 tcagcgttgc agagtgcggt aatcgggtac gcattgcggt gcgggatacc gggcacggca
     1621 tcgatccaaa ggtgattgag cgggtcgaag cgaacgaaat gccgggcaat aaaattggcc
     1681 tgctgaatgt ccatcatcgc gtgaagttat tgtatggcga ggggctgcat atccgccgcc
     1741 tggaaccagg aacggaaatt gcgttttaca ttcctaacca acgcacccct gtcgcctcac
     1801 aggctacgtt attgctttga gccggagtga tattgtgaaa gtcatcattg ttgaagacga
     1861 attcctggca caacaggaac tgagctggct aattaaagag cacagccaga tggagattgt
     1921 cggcaccttt gacgacggtc tggacgtgtt gaagtttttg cagcataacc gcgtcgacgc
     1981 catttttctg gatatcaata ttccgtcgct ggatggtgtg ttactggcac aaaacatcag
     2041 ccagttcgcc cataaaccgt ttattgtgtt tatcaccgcg tggaaagaac atgcggtaga
     2101 agcgtttgaa ctggaggcgt ttgactacat tctcaaaccg tatcaggagt cacgcattac
     2161 cgggatgctg caaaaactgg aagcagcctg gcagcaacag cagaccagca gtacgcctgc
     2221 cgcgacggta acgcgtgaga atgacaccat taatctggtg aaagatgagc gaatcatcgt
     2281 cacgccaatt aacgatatct attacgccga agcgcacgag aaaatgacct ttgtctatac
     2341 gcggcgtgaa tcctacgtaa tgccgatgaa cattaccgaa ttttgcagca aactgccgcc
     2401 atcgcatttt ttccgctgcc atcgctcatt ttgtgtcaat ctgaacaaaa tccgcgagat
     2461 cgaaccgtgg tttaataaca cctatattct gcggctgaaa gatctggatt ttgaagtgcc
     2521 ggtcagccgc agcaaagtta aagaattccg ccagttaatg catctttaat cgggaacttt
     2581 catgaaagca ccaggcttgc ccgccgatca acaatttttc gccgatctgt tcagcggcct
     2641 ggtgcttaac ccgcaactac tggggcgcgt ctggtttgcc agccagcctg cctcattgcc
     2701 agtgggcagt ttatgtattg attttccccg tctggatatc gtgctacgcg gcgaatacgg
     2761 caatctgctg gaagcaaagc agcaacgtat ggtggaagga gaaatgctgt ttattccggc
     2821 acgcgcggct aatttaccgg tcaacaacaa accggtgatg ctgttaagcc tggtgttcgc
     2881 accgacctgg cttggcttat cattttacga tagccgcacc acgtcgttgt tgcatcctgc
     2941 ccgccagatc cagcttccca gcctgcaacg cggtgaaggt gaagcgatgc ttacagccct
     3001 cacccatctt agccgttcgc cgctggagca gaatatcatt cagccgctgg tgttaagttt
     3061 gctgcatctt tgccgtaacg tggtgaatat gccgtcgggc aattcgcagc cgcgcggcga
     3121 ttttctctat cacagcattt gtaactgggt tcaggataat tatgcccagc cgctcacccg
     3181 cgagagcgtg gcgcagtttt ttaatatcac gcccaatcat ctgtcaaaac tgtttgctca
     3241 gcatggaacg atgcgtttta tcgagtatgt gcgttgggtg cgaatggcga aggcgaggat
     3301 gattttgcag aaatatcatc tgtcaattca tgaagtggcg cagcgttgcg gctttccgga
     3361 cagcgactat ttttgtcgcg ttttccggcg tcagtttggc ctgacgccgg gagagtacag
     3421 cgcccgtttt cagggctaac gtcagaaggt taattccgtt tccagcagcg tcagaatact
     3481 ttgcgcatct tgcgcggcaa acaacgactg gcggaagttt ttattcacca gtttgcgcgc
     3541 cagctgcgag aagactttca catggttcat cccttcgtta gcacccagcg tcagcatgat
     3601 caccagttcg acttcgccca tctctgactg ccagtcaacc ggtttcacca gccgggcaat
     3661 gctgatactg gaatgacgga tccactgaga tttggtgtgc gggatcgcca cgccaaaacc
     3721 aacgccagta gtgacaatct cttcccgctg ccagacatct tcttccagct caaacggacg
     3781 ttcagtacgc ccgttaacgc ccaggttgcc gcacagaaac tggatcgcct gctctttatt
     3841 gctaaaggcc tgatcaacaa agatattctc cagcgccagc agcgggcgaa cgtcttcttc
     3901 cggcgtaaac gcggtgagta acgcttcaat ctcctgcgcg ctgcggcatt cacatgcctg
     3961 acgcgccagt tcccgacacg cctcgctatc cagttgacga agctggcttt tcaccgccgg
     4021 aatgcgcggg ctactcatac tcagctcatc caggcccagc ccaagcagta gcggaagata
     4081 acggctttca ccgcccagtt caccgcaaat acctacccat ttaccccgct gatgcgcagt
     4141 ggtaacaatc tgctgcaaca tgcgcaggaa cgatggcgta atcgggttat acagcggcga
     4201 tacgcgcggg ttattacgat cgaccgcata cagatactgg gtcatatcgt tagaaccgat
     4261 actgaagaaa tcaacctcat cgcagaagtg gtcgatgatg tagcacaccg acggcacttc
     4321 caccatgata cccagcgtaa tcgtctctgc atgacgcaga ccatcgcgct taagctcaac
     4381 gatcgctttt tggagctcac ccttcaccca taagatctga tcgagactgt ggaccattgg
     4441 gatcatcaac tgggcgttgc cgaaactggc agcgcgcaaa atcgcccgca attgagtacg
     4501 gaacaggcca gcaaattccg ggtaaatacg taccgcgcga tagccgagga acgggttctc
     4561 ttcctgggga atgttgaggt aaggaatgct tttatcgcca ccgatgtcca tcgtgcggaa
     4621 gataatcggc ttgtcgcccg ccgccagtag cacctgctgg tacgcttcaa actgttcctg
     4681 ctcgtccggc acgctgtcgc gatccatata gagcatttcg gtacgaaaca gcccaacgcc
     4741 ttccgctccg ttggcaaacg cgcctggggc ttccagagct gtgccaatat tcgccgcaat
     4801 atcaatacgc tttttatcac gggaataggc cagctgcgcg gcagcttgcg cctgttgttt
     4861 ttggcgttta tccgccagcg tctgcgccac ctggtaataa ccgctcaccg catcgttggg
     4921 attaattgcc agtacgccgc actgggcatc aagcactgca ggttgcccgg cataacgggc
     4981 aatcgcatcc agcggcaggc cgctcagtac cgggatcgcc gaagcgcggg ccagaatcag
     5041 ggtatgcgag gtgcgcccgg ttttctccag aatcatgccc gcaagatttt ttaaatcgag
     5101 gctcaaaaac tggcttgggg ttaaatcttc agccaccaga atggtcggtt tttcaagcac
     5161 cagattattg cgcggtttta gttccggcca ggtgatatgc agcaactgtt cgctgatatc
     5221 gcgaatgtcg ctgacccgct cgcgcagata atcgctagca gaagcagaaa gtttggcaca
     5281 aacctgctcc atattgctga tgatcgccgc ccccagcccc tgatgctgtt ctgccatcag
     5341 gtgacggata ttgcctgcaa attcatcatc ctgaatcagc gacaaatggg cgctgaggat
     5401 agttttgctt tcgccgtcac gctcacgcaa ttgctggttc agttgctcgg caagcgttgc
     5461 cagactgtgc tccagtaggg tggaatcttg cgcactcgca gggatcaccc gataactgtc
     5521 gaggctgtcg ctctgtaaca gggtcagtgt acccacgcca acgccgcttg ccagcacgtt
     5581 gccgtacagt aaatccgggt tcaggcggct taatgaacgc ggcagcggat gcgccgtcag
     5641 ttctgccagc gtcggctgga cgctatcgct gtcgataaag cgcacctgga tatactcttc
     5701 cagcactcgc cgcgcctgct cttcatcgct accgctaatg ttcagactgc aactgtcatt
     5761 aaatagggtg ccagtgccaa tcagcgccag cgagcttttc gcatcggcct ttgcgttctg
     5821 gcgatggtta ataaaagtga tttcgctttg ccactggctg cactgctctt taagttccca
     5881 cgccggacga gcatgtagac cgttaggcaa aggacagaga aattgaatcg ttaacataac
     5941 cgctccgggt taatcaggag atcatctgaa atccgtcagt tgaacaaccg tctcacgcgt
     6001 aagacgttga ataagggcag ataaaagctg ctgcatctgc aaaatgtcac ggcaatcggc
     6061 aatcgatgcg gcgcaatgtc cgtggcgggt ggcaggcccc atcaccactg tgggtacgcc
     6121 agtgccggtt aagtgcaccg ccccaccgtc cgtgccaccg ttgctgaaca tatccgcctg
     6181 caacggcacg ccaatttctg ccgccacggt ttcaatccac gcggtaagtt ttggcggcgc
     6241 aatcagcgat ttatcgctta acaccagcat cggcccgtta ccaatctgac gatggttagc
     6301 cgcgccataa tcaaagtttt tcgcccagca ggcggtatca agcacaatgg cgacgtccgg
     6361 cgacaccgcg cgggtggcag tttgcccgcc gcgtaatccc acctcttcgc tggaacttgc
     6421 caccagccac acctccgcag gcagttcggc gtcgtgcagc tcacgtagta acgtcaccag
     6481 cagatagcaa ccgaggcgat catcaaaggc tttccccatc acccgctggt gagggagaac
     6541 ctgaaaagtt gtatcaaagg tgacgcgatc gcctggacga attcccgcct gcatcacttc
     6601 gtcatacgag cgcgcaccaa tgtccacgcg catggtgctg acgtcattcc cctgccggtc
     6661 gccgtcaagc aggcctggaa ttttgcactc ttcacgggta gtgatacgca ccgactgaag
     6721 ctggcgggca gccatgcgta cattgccaac cggcaacaca tcgatcgccc cttcgcggga
     6781 gatgctgcgc accataaatc ccacttcgtc catatgcgca cagatcatca ccttcggacc
     6841 tgtcgactca ttgagtcgga tcagcaccga tcccagccca tcaaatcgca cttctttttg
     6901 caggcgatcc gcttcttcca gcaggatctg ccgtacttcc tgttccgagg aggcgatcgc
     6961 atctgcctcg ctcaacgctt ttaataacga taaatccatt atgcctctcc cgtgagcaac
     7021 actgttttcg gcatggcgta gagcacttct gcaccttgcg gggtgaccag cacaacatct
     7081 tcgatgcgca cgcccccttg ccctggcaaa taaatccccg gctccacggt cagtaacatg
     7141 cctggctgta gcgtcgtggt gtcccgcggt gaaaaacgcg gatcctcatg gacttcaata
     7201 ccgatagcat gaccggtgtt atgaccgaaa tagtcgccat aaccggcttc tgtaatcacc
     7261 cggcgcgcgg cgtcgtcaac ctgctggcag cgtacgccgg ggcgaatcgc ggagattgct
     7321 gcgagctgtg cctgcaggac aatctgatag acgttaaaca gcgggtgaga ttcggcgctc
     7381 accccttcgc cattcaccag caaggtgcgc gtcatatcag agcagtaccc ctgatagagc
     7441 gcaccaaaat caagagtgac aaactcgccc gctgcaacaa tcttgtcgct ggctttaccg
     7501 tgcggcagcg cgccacgcca gccgctggcg acaatggtgt caaaagaagc tttttctgcg
     7561 ccctgctggc gcataaacca ctccagttca gcggctatct cgcattcgct catacccgcc
     7621 tgaataaagc ggcgaatatg ctctgcgcca cgatcggcaa tcccgcaggc aaggcggatt
     7681 ttctccacct cttctggcgt tttgatttgc cgcagcacat ccggcgtggc gctcaccagt
     7741 ttcgcattga gttcgaactg ccagcggtgc gcggtttccc aactcacctg ctggccttca
     7801 aaaccgagcg tttgcaactg ctcatcggca atgatttgat tgacgatagt ggtaagcgtg
     7861 ttcgtcgcgt caagcaaatg cagctggtag ccttgcgtgc gggcttctac atccgcgtaa
     7921 tagcgcgagt ccaccagaat gtgcgcactt tcacggctaa tcagcacata gcctgatccg
     7981 gtggagatcc ccagatgcgg ctgtttgttc tgccgtgagg agagaagcac cgcatccagt
     8041 tgttgcgcct taagccagtc gcgcagcgaa gcgagtaatg tcatcaggat tgtccttctt
     8101 acaggctatc gattaacaat ttgcctttac ggaacatcat cagacgcagg aacaccacca
     8161 tcagcgcagt tatgactgcc cccagcgcga tccccgccat gtagacgccg aggttagtga
     8221 ccagcggcca tgcccagata gcggactccg ggaaccattg cactgcgccc agccagacag
     8281 cggcggttga gccgacaatc gcgccgacca tataggacgg aatggcggtg atggggcttt
     8341 ccagcgcaaa cggaatcgca ccctcactga tccccataaa cgccaggaac atggcggttt
     8401 taccctgcgg ataaagctga gcactgaaca gacgtttgcc ggttaaacgg cggtcgataa
     8461 tggtcgccag acccagacca atcggcggaa taacaatagc gatggaacgc gcggtaaccg
     8521 gtaatacgtg gtcagtggta aagctgaagg caacgaaacc agctgctttg ttgatcgggc
     8581 caccaaggtc gattgctgtc gccgcggcga tccccatcgc gtacattagc gcacccttct
     8641 cacctgcggc agtcagcacg gtacggatac cgccgttgat ccagccacca aagggggtga
     8701 tgacgtagta catcgccagc atgacaaaaa tggcggaaag aatcggtagc aaaaatgtgg
     8761 ttttgaacgc taacaggaaa tccggtaact ggattttctg gttcatccac ttcacaaggt
     8821 aaccagcaac gatagaaata atcagggcac caatgaaggt ggacggcacc ggcgaagagg
     8881 tcgcccattg catcgtgctg ggatcgaagt tcagcagttg cgtcggctgg gtagacatca
     8941 acccaccgat aaaaccagcc gggaacgcca gtttgccacc gatagagttc gccacaaatg
     9001 cagcaaacat cggaatggca aaaccaaaca acacgccgcc gaaggactgc gacaaccagg
     9061 caaatttcag taaggagagg tcgaaaccgg agaatttccc gctattaaga gcatccatga
     9121 tgccgatatc agcaggaatt ttaagccagc tataagcaat cagctggctg aaagcgagga
     9181 tcacaccgcc cataattaag gtcggcacca tgcgtgaaat ccctgacatc acatgctgcg
     9241 ggagttcacc ccagaagctg gttttagagg cctgcggatt cttaactgcc gctgccgcac
     9301 cggatgcgcc aggcacaacg gttgcactgc gttttttaat ggccataaca attatccctg
     9361 tcgaatatta ctgttgttca gaggcaatca tctcttcgat ttctttgatg atgcccgcag
     9421 cgtttttaat tgcgtcctgc aaagtgattt cataaacgtc gcgtgattcg aaacgttcgt
     9481 tatcttccgg ggtaaccgcc acggagtgga tgatgatggt cgcttcggcg atatcctgcg
     9541 cggtcaggcg attctggatg ccgtccgcgc cctgagtttc aattttcact tcatagcctg
     9601 cttccaccgc cgcttcttcc agcgcctggg ctgccataaa ggtgtgagcc agacccatcg
     9661 ggcaggcaca taaagcaatc agtttcttac tcatcgttaa ttcctcgttc agtgatagtt
     9721 gacgagtgaa attatgaacg tgcaaaacaa atcgccgtta ccagacaaat aatgcattta
     9781 ctggagaatt aatcccgctg tacggaagtg tgacgcaggt cggccttgtg aggggacaca
     9841 aaggaaaatt gaattaactg tatgatttaa aagattatcg ggagagttac ctcccgatat
     9901 aacaggaagg atttagagaa tgtgacctaa ggtctggcgt aaatgtgcac cggaaccaag
     9961 aaggcccgga ttgtcatgga cgatgagata caccggaata tcatggacat attctttaaa
    10021 gcgcccttta tcttcaaatg cggcacggaa accagaggct ttgaagaact caaggaagcg
    10081 cggcacgata ccacccgcaa taaacacgcc gccaaatgtc ccgagattga gcgccagatt
    10141 gccgccaaaa cggcccataa tgacgcaaaa cagcgacaac gcgcggcggc aatcggtgca
    10201 gctgtcagcc agcgcgcgtt cggtaatatc ttttggcttg agattttctg gcaggcggtt
    10261 gtcggcttta acaattgcgc gatacaaatt caccagccca gggccagaaa gcacgcgctc
    10321 tgccgaaaca tgaccaattt ctgcacgcag tatttcgagg ataatgccct cttcttcact
    10381 attcggcgca aaatcaacgt gaccgccttc gcctggcaag cttacccaac gcttatctac
    10441 atggaccaga tgcgcaaccc ctagccccgt tccggcaccg taaaccgcaa taggcttacc
    10501 ttctaccggt tctgcgccac caaactgaat cagatgctct tttttcagca tcgggatcgc
    10561 catcgataca gcggtaaaat cgttgataat ttccagatgg ctaaaaccga gattcttttt
    10621 catttcggca attgagaacg cccaggtatg gttggtcatt gccacccaat caccggtaat
    10681 cgggcaagcg atggcaatac agccgtcttt cacctcgacc ttatgttctt caagataaac
    10741 gcgaatgacc gcttcgaggc tgggataatc aagccctgaa taggtcttag cctgcgagat
    10801 ttcaccactg gcaatatcac acagagcaag acgcgcgttg gtgccgccca catcaccgac
    10861 taatgcatac tttgtcattc ttcaactgct ccgctaaagt caaaataatt ctttctcaca
    10921 ctgtaaatac ctgggggcat aacaacaacg ccggaaaggc aggctccctg taaatatcga
    10981 tctgggtcac acaattactt tatcgtttca gcaccaattg cagcgatgcc tttttgcaag
    11041 ctgggcaaac taagtatctg accccgcata aggaatagaa catgctccat ccgcgagcca
    11101 gaaccatgtt gttattatcg ctccccgccg tggcaattgg gattgcgtcc agtcttattc
    11161 tgattgtggt gatgaaaatc gcctcggtat tacagaattt gctctggcta cgactgccgg
    11221 gaactctggg gatagcccag gattcacctt tctggatcat cgccatatta acgctaacag
    11281 gtattgcggt ggggttggtt atccgtttca gccaggggca tgctggacca gaccccgcct
    11341 gtgaaccgct gatcggcgca ccggttccgc cctctgcgct accaggactt atcgtagcat
    11401 taattctcgg tcttgctggc ggcgtcagcc tggggccgga acatccgatc atgaccgtca
    11461 atatcgccct tgcggttgcg ataggtgctc gtctgttacc gcgcgtcaac cggatggagt
    11521 ggactatttt agcctctgct ggaactatcg gcgcgctgtt tggcacacct gttgcggcgg
    11581 cattgatatt ttcgcaaacc ttaaatggca gtagtgaagt tccgctatgg gatcgtctct
    11641 ttgcgccgtt aatggcggca gcagctggtg cactaactac cggattattt ttccatcctc
    11701 atttttcact gcccattgcg cattacggac agatgcaaat gaccgatatt ctcagtggtg
    11761 caattgtggc ggcgattgcc atcgcagcag ggatggtcgc cgtatggtgc ttaccacggt
    11821 tgcacgcgat gatgcatcaa atgaaaaatc cggtgctcgt gctgggtatt ggcggattta
    11881 ttctcggtat tctgggggta atgggtggac cagtttcgct gtttaaaggg ctggatgaga
    11941 tgcagcagat ggtggcaaat caggctttca gcaccagcga ttactttttg ctggcggtga
    12001 ttaaacttgc cgctctcgtg gttgctgccg ccagtggctt tcgcggtggg cgaatcttcc
    12061 cggcagtgtt tgtcggggtg gcattagggt tgatgctgca tgagcacgtt cccgccgttc
    12121 cagcggcaat aaccgtttcc tgcgctattc tcggcatcgt gctggtggta acacgcgatg
    12181 gctggttaag tctttttatg gcggcagtcg ttgtacccaa taccacattg ctaccgctgc
    12241 tctgtatcgt catgcttccg gcatggctgt tattagcagg taagccgatg atgatggtca
    12301 atcgtccgaa gcaacagcca ccccacgata acatttagca aaaaatgctc tcctttatga
    12361 tttaagagtt atggcgatga tacggggctt tacctccccg taatattgcg ttaacaggcc
    12421 gctgacggct tcgatcgtga aggagaataa caatgttcag gtcactgttt ctggcggccg
    12481 ccctgatggc atttaccccg cttgcggcaa acgcaggtga aatcacccta ctgccatcaa
    12541 tcaaattaca aattggcgat cgcgatcatt acggtaatta ctgggacggt ggtcactggc
    12601 gcgaccgtga ctactggcat cgcaattatg aatggcgtaa aaaccgctgg tggcgtcatg
    12661 ataatggcta ccaccgtggc tgggataagc gtaaagcgta tgagcgtggc tatcgtgaag
    12721 gctggcgcga tcgtgacgat catcgcggaa aaggccgcgg acatggacac cgccattaaa
    12781 cacattcgcc aatggagcac aatgcctgat gcgacgctag tgcgtcttat caggcctaca
    12841 aaccggctct ttaacgcgta gggcggataa ggcgtttacg ccgcatccgc caatagtgcc
    12901 agatgcgacg ctcattcaac tacaatccca gcgccgtccc caccaacaac cagatattca
    12961 gcgccacgac cagcactaca atcacccagc ctgtctgttt tacgcgtttg ctgttcacca
    13021 gatcgcccat catcttgctg tcactggtga aaatcagcag tggaaccagc gccagggcga
    13081 taccaaaact taacagtacc tgactcataa ccagaatccg tgtcggatct aatcccatca
    13141 gaatcacaat aaatgacggc aacatggtga ctgtacgacg cacccacagc gggatatgga
    13201 agcgaatgaa gccctgcatc accacctgcc ccgccagtgt ccccaccacc gttgatgaca
    13261 gtccggcagc aaccaggctt aatccaaaga ccgttgccgc agcatggctt aacaacggtt
    13321 gcagcgtcag gtaagcctca tcaagatcgg caacgccagt atgaccggaa aagtggaacg
    13381 ccgccgcagc cgtagccatc atcgccagat tgacaaaacc ggcaatagtc atagcgatag
    13441 ccacatccca tttggtggca gaataacgtt gttgacgcga accaccatgt aaatgctgag
    13501 tcagcgaaga gtgtaaataa atcacgtgtg gcataatcgt cgcccccaac acacctgccg
    13561 ccaggaagac tgcctccgag gtgggtaaac tcgggattac cattccttta cccagttgcg
    13621 ccaggttagg ctgggagaaa atcaactcga caatgtaagc cgcagcaaca aacagcagta
    13681 acccgccaat cactttctcc agcggttttt gccctcgacg ttgcagcatt aaaatcagga
    13741 aagtcgcgat ccccgtcagc accgcgccct gcaacagcga aacacccaga atgagtttaa
    13801 aaccgatcgc cgcaccaata aattccgcca ggtcggttgc catcgcaatg atttctgcct
    13861 gaacccaata gaaccacact acgggacgcg gatagtgatc gcgaatctgc tccgccaggt
    13921 ttttaccggt ggcaatcccc agtttggcag acagtatctg aatcagcatc gccatcaggt
    13981 tggcccaaac gacaacccac agtagctgat agccgaagct ggcacccgcc tgaatattgg
    14041 tcgcaaagtt accaggatcg atataaccaa tcgccgcaat gaacgcaggt cccattaatg
    14101 cgagcctcat cttgcgcgcc gcccgtccgc tgctactctc aacgcgatag ttagtcatct
    14161 tgtgcctcta aaacatagcc tttgctatgt ttcatgctat gtcaaacgag aatgattatc
    14221 aaattcattt aagtggattg tgatgatttc tttgatagac cagaattatg acgacgaaaa
    14281 gattgatgac ttgaatgttg tgattatgtt taatgttagc acatttacat aactttcagc
    14341 ttccatacac aacatagcag aaatgtatga cagctcacta tttttgaagc ttgtcacagg
    14401 acgtcattat agtgtgtgtc agatctcgtt ttccttaacc atgttacata gaatgtgcac
    14461 ggaaatttaa cctgcctcat atttggagca aatatggacc gcgtccttca ttttgtactg
    14521 gcacttgccg ttgttgcgat tctcgcactg ctggtaagca gcgaccgcaa aaaaattcgt
    14581 atccgttatg ttattcaact gcttgttatc gaagtgttac tggcgtggtt cttcctgaac
    14641 tccgacgttg gtttgggctt cgtgaaaggc ttctccgaaa tgtttgagaa actgctcgga
    14701 tttgccaacg aagggactaa cttcgtcttt ggtagcatga atgatcaagg cctggcattc
    14761 ttcttcctga aagtgctgtg cccaatcgtc tttatctctg cgctgatcgg tattctccag
    14821 cacattcgcg tgttgccggt gattatccgc gcaattggtt tcctgctctc caaagtcaac
    14881 ggtatgggca aactggaatc cttcaacgcc gtcagctctc tgattctggg tcagtctgaa
    14941 aactttattg cctataaaga tatcctcggc aaaatctccc gcaatcgtat gtacaccatg
    15001 gcagctacgg cgatgtccac cgtgtcgatg tccatcgttg gtgcatacat gaccatgctg
    15061 gagccgaaat atgtcgttgc ggcgctggta ctgaacatgt tcagcacctt tatcgtgctg
    15121 tcgctgatca acccttaccg tgttgatgcc agtgaagaaa atattcagat gtccaacctg
    15181 cacgaaggtc agagcttctt cgaaatgttg ggtgaataca ttctggcagg tttcaaagtt
    15241 gccattatcg ttgccgcgat gctgatcggc tttatcgccc tgatcgccgc gctgaacgca
    15301 ctgttcgcca ccgtgactgg ctggtttggc tacagcatct ccttccaggg catcctgggc
    15361 tacatcttct atccggttgc ttgggtgatg ggtgttcctt ccagtgaagc actgcaagtg
    15421 ggcagtatta tggcgaccaa actggtttcc aacgagttcg ttgcgatgat ggatctgcag
    15481 aaaattgctt ccacgctctc tccgcgtgct gaaggcatca tctctgtgtt cctggtttcc
    15541 ttcgctaact tctcttcaat cgggattatc gcaggtgcag ttaaaggcct gaatgaagag
    15601 caaggtaacg tggtttctcg cttcggtctg aagctggttt acggctctac cctggtgagt
    15661 gtgctgtctg cgtcaatcgc agcactggtg ctgtaagacc atacataaaa aagccgggga
    15721 taattccccg gttttttacg cctgttaatc agctaatggc tgcgggcgac caatcaaata
    15781 cccttgcaga tattgcaccc cgagcttatg caatagcgcc tgctgctgtt gcgtctcgac
    15841 aaactccgcg accacactca atgacttcgc tttcgccaga tcggtaattg atctcacaat
    15901 catcgcatcc agcgtattcg tgacgatatc tttcacaaag acgccatcaa ttttgatgat
    15961 atcagcctgc aaacgtttta accgttcgta gttggcatag ccggtgccaa agtcatcaat
    16021 cgcaatccgg aaaccaaact tatgcagttg ctcgatgttg tacatgctgc tttctgcgtt
    16081 agaaaacgcc tgctcctcgg tgatctcaag aatgaccgcc tgcggggaga tgtgataacg
    16141 cttaaacaga cgaataatcc gcccggcaat attcttttgc agcagcgtga gcggcattaa
    16201 attgactgaa aagcgcgggc cttttttgtc gcaaggatgt gttgccagcc acttcaacag
    16261 ggattccagc acttgcaaat caaaacgcgc gctaaggtta aactgagcaa taaggggcag
    16321 aaacttatcc ggggtcataa tgccgccgtc atatttcagt cgcgcgagga tctcatcata
    16381 accttcaccc tctttgttgc gaattggctg ggcgtagagc agcaaatcac cctgatctaa
    16441 cgccgtacga atggtattca gcaatagcac ctgtttagtg gtctgcccgg aaaccatctc
    16501 ctctctgcta tccagcgcca gcacatggtg atgtgcgcag gattgctccg ccagccagct
    16561 taactgcccc aacaagggtt gcaaggtttc ctgatttcca tcaaaacgcc cccaggcagc
    16621 accatagccc atatccagcc cggtattgtt ccagtgaatt tgccgactat tcaggatgtt
    16681 aaccatatgc tggagtcgcc cttccgtttc cggtccactc agcactaaca gcagttcact
    16741 acccggcaat tgatacaact tttcgttttc ctgcatcaac ggcagcagcg tacggtaaat
    16801 tgatcggata cagtgaacgc gcatcattaa gccgtaatga cgactcataa actcaagatt
    16861 atcaatgcgc aggcagcaaa aactcttgcc cgcctcttgc tccggcgctt gctccaacgc
    16921 acgaaagttg ggtagaagcg ttaacggatc ggtcagcgcc tgcaaatgcc agcggcggtt
    16981 aagccattca ctacgatgat aaatacgcac catatagagc aggcaaacac taaaggaaat
    17041 cagcaccgcg agaataaatg ccagcgaata ttcggttagt accccttgca aaaagttctg
    17101 gttgtaattc agcaggcaaa gcgttgaaac agcccaggtg agatttaaaa acggatagcg
    17161 aagcttaccg acaccgaggg taaagatgat aaagaaaaca ggtaccaggt aaccggcaat
    17221 aaagtcattc tcatacggtg tgcacatcag aagtagcagc acgctaagag ctgccagcca
    17281 gcttaaggta aatgcgcgtt tctctttgct caacgacgga gcaatatccc tgcgccacaa
    17341 tatctgcgca aagtggggac ttacaatcat gcgagtgaga tagtagaaaa gcatgttgta
    17401 aatcagcaca gcggtgaaaa ggcttagtaa atcaacgact gtgaaaatgg catccgcatc
    17461 gccgaaaaag gtggatatct ttagcgggaa atcaaaaaag ctccccacaa gatacatgct
    17521 gcatttgatg ccgatcggcg tcaccaaacc aagccagaaa agacgttgcc agacatatcg
    17581 gctggtcaat ccataacgcc aacgcgtccc cagctgccag cgtaatattg cgcaggcaca
    17641 caacaccaca aacgtctggc tgccgagcat gaccgcggtc tgtaaaaaag agagatgaaa
    17701 gttccacaga ttggtgcaaa acatcccgca taaaacgggg acgacaccgc gccagccaaa
    17761 gataaacaag attgacagca ttacgcacag tggcatccac gccagaaaga tataactgga
    17821 atggataatg gcaagtggcg aaataaaacg ggatagctga ataagtacca cggtgagcgt
    17881 aaacgctagt gtgaatatct tgatattttt tatcaggtta tgctccacaa acatgagtac
    17941 actactctca ctgatcgggg ttttcaggca cacaaataat aagtttattc gcatcgcgaa
    18001 gcaaacaggg gccagcctgt tgccgatttt tcatccaatg atgctttcac caggcggcgc
    18061 taacccggac gtagactgta ttacaaaagc ggcaaaaagc agagacaaaa aacccccgct
    18121 ttacagcgag ggtttgaaat ttggtggagc taagcgggat cgaaccgctg acctcttgca
    18181 tgccatgcaa gcgctctccc agctgagcta tagccccacg atgcgtttac gtaccaaatt
    18241 tgctgggtgc aaaatttggt ggagctaagc gggatcgaac cgctgacctc ttgcatgcca
    18301 tgcaagcgct ctcccagctg agctatagcc ccgtcacgta aagcttgtcg agttgacggg
    18361 cggcatcata tgaattccgc ccgaatgtgt caacggcaaa ttgcaacgtg tagtttcaat
    18421 cgctgaaaaa tcaggcaaat gaacaatttt ggaaaaccgc tcgcattcag tagttattca
    18481 tgtcacggtt tcatgtaaag tggtgttata aaatgaacta ctaatagacc cacatacatt
    18541 cagggaattg ttatgttcaa ggagaggatg acgccagatg aacttgccag actgaccggc
    18601 tatagccgcc agaccattaa taaatgggta cgcaaggaag gctggacaac gtcaccaaaa
    18661 cctggcgtcc agggtggcaa agcccgactg gttcacgtca atgaacaggt tcgtgaatat
    18721 attcgcaatg ctgaacgtcc ggaaagtcag ggagaaacgc ctgccctttc tggtgatgcg
    18781 ccgcttgaag tattgctggt gacactggcg aaagagatga cgccagtcga acaaaaacag
    18841 tttacatcct tgcttctgcg ggaagggatt atcggattgt tacaacgctt agggattcgc
    18901 gatagcaaat aatatgaaaa gattacgcaa taaaatgacc accgaagaac tggctgaatg
    18961 cctcggtgtg gctaaacaaa ctgttaaccg ttggatcaga gaaaaaggct ggaaaacgga
    19021 aaaatttcct ggcgtgaaag gcggtcgtgc cagacttatt ctggtcgata cgcaagtttg
    19081 cgagtttatt cagaacacgc cagccttcca taacacgccg atgcttctgg aagccgaaga
    19141 accccttgcc gaatacgcac cggggattcg cactcctgct tatcggcaaa tcatcagcgc
    19201 aatagacaac atgactcaca gtgagcagga aaaagttgcg caatttttgt cacgcgaagg
    19261 aattcgtcat ttccttgctc gtctcgacat agatgaatca gaataaataa aaaacggcag
    19321 gggtatctct cctgccgttt atcttttatg cgctaattat tgctgatttt cgcgttcagc
    19381 aataaaagcc agcgctttgt tgatacgctc gatactgcgg gtcttaccga ttgcgtgaac
    19441 ggtaacatcc agcgccggag actgccccgc accggttacg gcgacacgca gcggcatacc
    19501 aactttaccc atacccactt ccagctcatc cgccgtcgcc tgaatagcgt gatgaacgtt
    19561 ttcagcggtc cagtcagtaa tcgcagtcag tttgtcacga accacttcca gcggctgacg
    19621 cgctaccgga cgcagatgtt ttttcgcggc gtcggcatcg aactcagcaa aatcttcgta
    19681 gaaataacgg cagctctgtg ccatctcttt cagcgtcttg cagcgttcgc ccagcagttt
    19741 caccagatca gccagctgcg ggccgttacg ggtatcgata ttttcctgct caatgtgcca
    19801 ttgtaagtga gtagcaacat actccggcgg cagcgcgtta atgtagtgat ggttcagcca
    19861 cagcagcttg tcggtgttga acgcactggc agatttgctg acggcattca gagtgaagta
    19921 tttgatcatc tcttcacgag tgaagatttc ctgatcgccg tgggaccagc ccagacgcac
    19981 cagatagttc agcaatgctt ctggcaaata accgtcatca cgatactgca ttacgctgac
    20041 cgccccgtga cgtttggaca gttttttgcc gtcatcgccg ttgatcatag aaacgtgcgc
    20101 gtaaaccggt accggcgctt tcagtgcttt aagaatgttg atctggcgcg gcgtgttgtt
    20161 gatatggtct tcgccacgga taacgtgggt gatttccata tcccagtcgt caaccaccac
    20221 acagaagtta taggttggag aaccatcggt acgacggata atcagatcat ccagctcctg
    20281 gttgctgaac tcaatcgggc cacggatctg atcgtcaaaa acaacagaac cttcctgcgg
    20341 gttagcaaaa cgtacaacgc acggttcatc atcagcatga tgctcatggc tgtggcggca
    20401 gcgaccgtca taacgcggct tctcaccttt cgccatttgc tcttcgcgca gcgcttccag
    20461 gcgctcttta gagcaatagc atttataagc agtgccctct tccagcatct gatcgatcac
    20521 cgcgttgtag cgatcaaaac gtttggtctg gtagtacgga ccttcatccc actccaggct
    20581 cagccagttc atgccatcca taatggcttc gatagcttcc ggcgtggaac gctcaagatc
    20641 ggtgtcttca atacgcagca cgaactcacc gccgtggtta cgtgcaaaaa gccaggagta
    20701 aagagcagta cgcgcgccgc caacgtgcag atagcctgtt gggcttggcg cgaagcgagt
    20761 tttgattttc atgaaatggc cttacgttta gaaagatgcc gacaaccggc aaatcctgga
    20821 aaaattgagt gggcgatatt ctatcactcc cgcctgattc ctcaatgtag ttcgggttgc
    20881 aaccttacgc attattgttt tcgttgacaa attgcgcaac acggtctgtt ttgcgatcat
    20941 cttgcttaaa tttacgacga acgaacaatt tctttagaaa atgcgttgac tcattttgaa
    21001 ctctccctat aatgcgactc cacacagcgg gggtgattag ctcagctggg agagcacctc
    21061 ccttacaagg agggggtcgg cggttcgatc ccgtcatcac ccaccaacta ctttatgtag
    21121 tctccgccgt gtagcaagaa attgagaagt gggtgattag ctcagctggg agagcacctc
    21181 ccttacaagg agggggtcgg cggttcgatc ccgtcatcac ccaccacttt ctcgcca
//
LOCUS       DAPHCV010000089        21050 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.089, whole genome shotgun sequence.
ACCESSION   DAPHCV010000089 DAPHCV010000000
VERSION     DAPHCV010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.089"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(46..699)
                     /gene="fdnI"
                     /locus_tag="RH033_003330"
     CDS             complement(46..699)
                     /gene="fdnI"
                     /locus_tag="RH033_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HDV2992471.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPIQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(692..1576)
                     /gene="fdnH"
                     /locus_tag="RH033_003331"
     CDS             complement(692..1576)
                     /gene="fdnH"
                     /locus_tag="RH033_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HDV2992472.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(1589..4636)
                     /gene="fdnG"
                     /locus_tag="RH033_003332"
     CDS             complement(1589..4636)
                     /gene="fdnG"
                     /locus_tag="RH033_003332"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(4049..4051),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDV2992473.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGIANGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            4868..5749
                     /gene="yddG"
                     /locus_tag="RH033_003333"
     CDS             4868..5749
                     /gene="yddG"
                     /locus_tag="RH033_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HDV2992474.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            6022..7410
                     /gene="narU"
                     /locus_tag="RH033_003334"
     CDS             6022..7410
                     /gene="narU"
                     /locus_tag="RH033_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HDV2992475.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            7505..11245
                     /gene="narZ"
                     /locus_tag="RH033_003335"
     CDS             7505..11245
                     /gene="narZ"
                     /locus_tag="RH033_003335"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HDV2992476.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLADGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGCNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     QELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKREGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            11242..12786
                     /gene="narH"
                     /locus_tag="RH033_003336"
     CDS             11242..12786
                     /gene="narH"
                     /locus_tag="RH033_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDV2992477.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHNAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            12786..13481
                     /gene="narW"
                     /locus_tag="RH033_003337"
     CDS             12786..13481
                     /gene="narW"
                     /locus_tag="RH033_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDV2992478.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFALDLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLNCRE
                     LPDYLPLYLEYLSILPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     PLSSDSVTKQIRSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            13478..14158
                     /gene="narI"
                     /locus_tag="RH033_003338"
     CDS             13478..14158
                     /gene="narI"
                     /locus_tag="RH033_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDV2992479.1"
                     /translation="MIQYLNVFFYDIYPYLCGTVFILGSWLRYDYGQYTWRAASSQML
                     DKRGMVLWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAVKQQMAMILGGICGVLT
                     LIGGAGLLVRRLTNPRIRATSSGADILILAILLIQCILGLTTIPFSAQHPDGSEMMKL
                     VGWAQSIVTFQGGASTHLDGVAFIFRVHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            14238..15131
                     /locus_tag="RH033_003339"
     CDS             14238..15131
                     /locus_tag="RH033_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HDV2992480.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGETCAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPDKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLSHDGNVLRVKGHQGRALGRDGVIDVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIDF"
     gene            complement(15227..16072)
                     /gene="nhoA"
                     /locus_tag="RH033_003340"
     CDS             complement(15227..16072)
                     /gene="nhoA"
                     /locus_tag="RH033_003340"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HDV2992481.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVTAHRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVPDLVQITPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQNDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            16247..16816
                     /locus_tag="RH033_003341"
     CDS             16247..16816
                     /locus_tag="RH033_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDV2992482.1"
                     /translation="MSQFIPVELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFYCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQEKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(16820..17047)
                     /gene="pptA"
                     /locus_tag="RH033_003342"
     CDS             complement(16820..17047)
                     /gene="pptA"
                     /locus_tag="RH033_003342"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HDV2992483.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISISL
                     QQIQPESWQAIWDAEIEPQMEALIKKPGYSM"
     gene            17353..19329
                     /locus_tag="RH033_003343"
     CDS             17353..19329
                     /locus_tag="RH033_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDV2992484.1"
                     /translation="MQLNHIVKSLLITGLFTTSSLPLLAAEAPKDATAATQQANNLLY
                     NQLPFADNTDFTDAHKGFIAPIPQDVIKGEQGNVIWDPQHYAFIKEGDKAPDTVNPSL
                     WRQAQLINISGLFEVTEGVYQIRNLDLSNMTIIEGKEGITVVDPLVSAETAKVGMDLY
                     YKNRGKKPVVAVIYTHSHVDHYGGVRGVIDEADVKSGKVKIYAPAGFLEEAVSENIMA
                     GNVMSRRASYMYGNLLKPDVKGQVGAGLGTTTSAGTVTLIAPTNYITKTGQIETIDGL
                     TYDFLMAPGSEAPSEMLWFIEEKKLIETAEDVTHTLHNTYSLRGAKIRQPLPWSKYIN
                     EALNLWGDKAEIILAQHHWPTWGNDNVVKLLKSQRDLYRYINDQTLRMANQGMTRDEI
                     AANFKLPSSLANTWANRGYYGSVSHDVKATYVLYLGWFDGNPATLDELPPEEGAKKFV
                     EYMGGADAILQKAKQDYDQGNFRWVAQVVSKVVFADPNNQAARDLEADALEQLGYQAE
                     SGPWRNFYLTGAQELRNGVQKLPTPNTASPDTVRAMTPEMFFDYLAVHINGEKAADAK
                     TVLNFDFGEDGGTYKVELENGVLNHTAGVEASDADATITLSRDVLNKIVLKEETLKEA
                     TAKGDVKITGNAEKLNELLGYMDNFEFWFNIVTP"
     gene            19424..20473
                     /locus_tag="RH033_003344"
     CDS             19424..20473
                     /locus_tag="RH033_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-associated sensor domain-containing
                     protein"
                     /protein_id="HDV2992485.1"
                     /translation="MVIRGMTISLPWFAFINVSFALIILLRRVLFNDLTPPWLNEKSL
                     IHSIDISATGILLICSGLLLIPRQKTLPIQVLLVALSLLWSWCSYHFIAYWTLQFAYP
                     LCVLLMLSGVVALYFHTPSLLAFVIPLWFTTPIASLMLNQQINIHFAVVWCIFSLALY
                     GGRLILLRWFEEAWVQNSYNNQLINRLDALAHRDPLTGIANRRAMNSILHDAIDNGGS
                     FALIMLDVDFFKRYNDTYGHPAGDRCLIQVADALQRSTRQSEDVVVRYGGEEFAIILF
                     NATLPEAETVAARVKQELKLAAIPHQASAVNAFVTVSQGITCSAPAKTAEQIISDADA
                     ALYRAKESGRNRWAL"
     gene            complement(20578..21050)
                     /locus_tag="RH033_003345"
                     /pseudo
     CDS             complement(20578..21050)
                     /locus_tag="RH033_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         5071 a         5406 c         5278 g         5295 t
ORIGIN      
        1 tggcgcttct taaagcgcca ttttcagctt aaagttcaaa agagattata tcccttcttc
       61 actctctttt ttcgcttctg ccttctcgat ttcacgatac cagcgcggat ggtgtttctt
      121 cgcccagcga cggctcacct tcccttcgat catcccttta atcgatcctt tcacccagaa
      181 tgccatatac atatggatca ggatggcgtg gatcaggatg atacccgcag ccgcatggat
      241 cagcaggcta taacggacaa cctgtatcgg gaagtactgc gcaaagtacg gacgccagat
      301 aatcaccccg gtcaccagca gcacgaaaat catgctcatg atcgaccaga acatcatctt
      361 ttgcccggcg ttgtacttac cgacatccgc cactttatgc tcattgcctt tcaatacttc
      421 gacaatgttc aacagccacg gaatatcttt cttatccggg atgttgtgat gcacaaaacg
      481 cacaaacata aacatcagtg cgacgaaaat cgcaatgccg aagaacgggt gcaaaatgcg
      541 tcccatctgc ggcgtaccga aggtttgcgt cagccattgc agcgtcggga agaagaacga
      601 aatcccggac agcgccacca ggaagaagca aatcaccacg gtccagtgac aggcgcgatc
      661 aataaatttg gtgcgcacaa tcattttcga cttactcatg atgatcctcc tcgtcatcgt
      721 ccacttcctt attcgggcca ataccgatgt agtggaaaat caacccggca aaagtggcaa
      781 taaagccagc cgctgccagc ggtttcaacg cgcctttcca caggcttacc gaggtgtcga
      841 tcttcggatc tttcggcaga ccgtgataca gctccggctg atcggcgtga tgcagcacgt
      901 acataacgtg cgtaccaccg accccttccg ggttgtagac gccagcatgt tcgtaaccac
      961 gcgctttcag tttcgccacg cgctgttccg ccagctccag catctccttc ttggtgccga
     1021 agtggatagc cccggtcgga caggttttca cacaagccgg ttcctggccg acgctgacgc
     1081 gatcgacgca gagcgtgcat ttatataccc ggttatcctc tttgttgagg cgcggaatat
     1141 taaacggaca cccggcaatg cagtaaccac agccgatgca gttttccgac tggaaatcga
     1201 caatcccgtt agcgtactga atgattgcac cagcagacgg gcacgccttc aggcagcccg
     1261 gatcttcaca gtgcatacag ccgtctttac ggatcagcca ctccagcttg ccgttctgtt
     1321 cggtttcgct aaagcgcatc accgtccagg acttggcgct cagatcggcg gggttatcgt
     1381 aaaccccgac gcagtgcccc acttcatcac ggatgtcgtt ccactctgaa cacgccacct
     1441 gacaggcttt acagccgata caggtggaaa cgtcgataag tttcgcgact tctgctttgt
     1501 aatcacgcac ctgagaaggc ggcgtgatgg agttagttgc ggaccttttg ataatgtcct
     1561 gcgtttccat agccatctgt tcgccccctt acgccttctc gatgttgact aagaacgctt
     1621 tatattccgg cgtttgcgag tttgcatcac cgacattcgg cgtcagagtg ttagcgatat
     1681 aacctttacg cgcgacaccc tcaaagcccc agtggattgg aatacccacc gtttcaacct
     1741 gctgaccgtt tacattcagc ggtttcagac ggcgcgttac cacagccacc gcgcggataa
     1801 agccacgctt gctggagaca gtgacacgat cgccattggc aatgcctttc gccgccgcca
     1861 gcgtttcgct gatttccaca aactgttccg gctgagcaat tgcgttgagc aatgcgtgct
     1921 tggtccaggt gtggaagtgc tcggtcagac gataggtcgt acccacatac gggaactgct
     1981 cttttttacc catccgcagc gcgtcttgtt catacagacg aacaaccggg ttagacacca
     2041 cgttcggatg cagcgggtta gtgcccagcg gcgtttcgat tggctcgtag tgttccggga
     2101 acggaccttc cgccattttg ttgatggcaa acaggcgtcc catcccttcc ggctgcatga
     2161 taaacggccc ggttggcgta cccggtgcgg cattgccgaa gtccggaata tcgttacccg
     2221 tccacttgct gccgttccac tggatcagca tccgtttcgg atcccacggt ttaccgttga
     2281 tatccgccga agcacggttg tacagcacgc gacggttgag cggccacgcc caggcccatc
     2341 ccagcgtatt ccccagaccg gacgggtcgg agttatcgcg gttagccatc tggttgccct
     2401 gctctgtcca gctaccggtg tagatccagc aagaagatgc ggttgtacca tcatcacgca
     2461 gatgcgcaaa gctactcagc aactgacctt tcttcgcaat cagcacgcca ttagcgtcat
     2521 agagatcttc cagcgcatag ccgttgttct ctttcgccac ttcgtcagat tgcggttcgt
     2581 gcggctgctt gtagttccag ctcatcttca tcagcggttc tacgccttta ccaccctcgg
     2641 cctggtacag ctcgcgcaga tgatggtaga tacccgccag aatttcgccg tcgttacgtg
     2701 cttcgcccgg cgcgtcctga cctttccagt gccactgcaa ccagcgaccg gagttagcaa
     2761 tagaaccatc ttcttcagca aagcaggtcg aaggcagacg gaatacttca gtctgaatgg
     2821 acgccggatc gacatcgttc gactcgccgt ggttctgcca gaaggtagag gtttcagtca
     2881 ccagcggatc gataaccacc atgtacttca gcttgctcag gcagctcacc actttgtttt
     2941 tgtccgggaa ggacgcaacc gggttaaagc cctggcagaa ataaccggtg actttgcctt
     3001 cgtccatcat gttgaaatac ttgatgacgt cgtaggtctg gtcccacttc ggcagccagt
     3061 cgtagcccca gttgttctct ttttgcgcgg catcgccgta gaaagatttc atcaggctaa
     3121 cgaagaactt cggatagttg ctccagtagt tcacctgatc agccagcgtc gctttcggcg
     3181 tattcgcttc cagatacgac tgcaaatcaa cctgtttttc tgacggcagc gtcagataac
     3241 ccggcaggct ggttgagagc agacctaagt cggtcaggcc ctgaatgttg gagtgaccac
     3301 gcaatgcgtt cacgccgcca ccggccatac ccatgttacc gagcagcaac tggatcatcg
     3361 ccatagtacg gatgttctgc gcacccacgg tgtgctgcgt ccagcccagc gcgtacagga
     3421 aggtggttgt gcgatccggt gcgctggtgg aggccagcac ttcacacact ttcaggaagt
     3481 cggcttttgg cgtaccgcag atgttttcta ctacgtccgg cgtgtagcgg gaaacgtgcg
     3541 ctttcagcag gttccacaca cagcgcggat gagtcagtgt ttcatcgcgt ttcgcatagc
     3601 cgttttcatc gaactgatag ttccaggacg atttatcgta ttgccgtttt tcagcgtcgt
     3661 agccgctgaa cagaccgtct tcgaaagcaa aatcatcacg caccagcagg ctggcgttgg
     3721 tgtaatgctt aacgtattcg gcgttgattt tgttgttttc gatcaggtag cgcaaaacgc
     3781 cagacaggaa cgtaatgtcc gtaccggaac gaataggtgc gtaaatatcc gccacagaag
     3841 cggtacgcgt aaaacgggga tcgacaacga tcaaggttgc atcgttgttg tttttcgctt
     3901 ccatcgccca gcggaaaccg acgggatgcg cttcagcagc gttaccgccc atcaccatta
     3961 cgacgttagc gtttttgata tccacccagt ggttggtcat cgcaccgcga ccaaatgttg
     4021 gagcaagact tgctaccgtt ggtccgtgtc agacgcgcgc ctggttgtct accgccagca
     4081 tcccgaggga gcgggcaaat ttttgagtca gcatcccggt ttcgttgctg gcaccggagg
     4141 cacacagcat accggtagaa agccaacggt ttaccgttac gccctgctcg ttcttttcaa
     4201 taaagttagc gtcacggtca gctttcatca gcttcgcaat acgggagaat gcttcttccc
     4261 agctaatgcg ctgccatttg tcagaacctg gcgcacgata ttccgggtag cgcagacggt
     4321 tttcgctgtt gacgtaatcc agcaaaccgg cccctttcgg gcacagcgca ccacggctta
     4381 ccggatgatc cgggtcacct tcaatgtgat aaatcgcttc tctggcgttt ttcgcgccat
     4441 cacccaggct atacatcaat agcccgcaac ctacggaaca gtatgtgcag gtgttacgga
     4501 tctctttcgc gcgtaataat ttgtagtttc gcgcctgagc cagtgcttgc ttcggggcaa
     4561 agcccaatgc tgctactgtt gttcccgcca taccgcccgc gcagatttta aaaaattgtc
     4621 tgcgactgac gtccattgct ttcctctttt ttcaggggta ttactgcgcg aggaaactaa
     4681 cagtaaaatt ctgaatcttt ttgcgcgaaa tcaaaaagaa cacgtgtttg ccacaaaata
     4741 atccgcctgc gcaaattgac ctacctcaat agcggtagaa aaacgcacca ctgcctgaca
     4801 ggccagttaa aaaaatgcta taaaattcag cttaattttt aacggcaaga gagacaaaac
     4861 agcgagcatg acacgacaaa aagcaacgct catagggcta atagcgatcg tcctgtggag
     4921 cacgatggta ggattgattc gcggtgtcag tgagggactc ggcccggtcg gcggcgcagc
     4981 tgctatctat tcattaagcg ggctgctgtt aatcttcacg gttggatttc cgcgtattcg
     5041 gcagatcccg aaaggctatt tactcgccgg gagtctgtta ttcgtcagct atgaaatctg
     5101 tctggcgctt tccttagggt atgcggcgac ccgtcatcag gcgattgaag tggggatggt
     5161 gaactatcta tggcccagcc tgacaattct ctttgccatt ctgtttaatg gtcagaaaac
     5221 caactggctg attgtaccag gattattatt agccctggtc ggcgtctgct gggtgttagg
     5281 cggtgacaat gggttacatt atgatgaaat tatcaataat atcaccacca gcccattgag
     5341 ttatttcctg gcgttcattg gggcgtttat ctgggcagcc tactgcacag taacgaataa
     5401 atacgcacgc ggatttaatg gaattaccgt ttttgtcctg ctaacgggag caagtctgtg
     5461 ggtttactat tttcttacgc cacaaccaga aatggtattt agcacgcccg tcatgattaa
     5521 actcatctct gcggcattta ccttaggatt tgcttatgct gcatggaatg tcggtatatt
     5581 acatggcaac gtcaccataa tggcggtagg ttcgtatttt acgcccgtcc tttcctcagc
     5641 gcttgcagcc gtgctgctca gcgccccgct gtcgttctcg ttctggcaag gcgcgctaat
     5701 ggtctgcggc ggttccctgc tctgctggct ggcgacacgt cgtggctaaa ataattgtcg
     5761 ggtctacggc ccggcagtta atcagataaa ctctgacaat attaaaaaat aaatttcaaa
     5821 taccagaaag tcattttagt tacatttagt aacacatatc tgtctgttta atccacattc
     5881 tgcccccgca gttacgcttt cttatctatt cttctaatca gcacttcacc tgattcatcc
     5941 ttaattctaa taactggcta ttgcgcctta cctcctgttt tgtctttttt tcattaccaa
     6001 tgtgtgcatg tgaggaacaa tatggcactg caaaatgaga aaaatagtcg ttatctttta
     6061 cgcgactgga aaccagaaaa tccggccttc tgggaaaata aaggaaagca cattgcacga
     6121 agaaacctct ggatatcagt cagttgtcta cttcttgcct tctgtgtctg gatgctattt
     6181 agcgcagtta ctgttaatct caataaaatc ggttttaatt tcactaccga tcaactcttt
     6241 ttattaaccg cattaccctc cgtttctggc gcattattgc gtgtccccta ctcctttatg
     6301 gtgcctatat tcggtggacg ccgatggacg gtttttagta ctgcaatcct gattattcct
     6361 tgcgtctggc tcggaattgc cgtgcaaaat ccgaataccc cttttgggat atttatcgtt
     6421 atcgctttgc tatgcggttt tgcaggtgcg aactttgctt cgagcatggg caatatcagt
     6481 ttcttctttc caaaagccaa acaagggagc gctcttggga ttaatggcgg attaggaaac
     6541 ttaggtgtaa gtgtgatgca gctggttgca ccgctggtca tttttgtccc cgtatttgcc
     6601 tttctcggcg tcaatggcgt accgcaggcc gacggttcgg tgatgtcgct ggcgaatgcc
     6661 gcatggattt gggtgccatt actggcgatt gccacgatcg ccgcctggtc agggatgaat
     6721 gatatcgcca gttcacgcgc ctcaattgcc gaccagctgc cagtgttaca acgcctgcat
     6781 ctctggctgc tgagcctgct ttaccttgcc accttcggtt cgtttatcgg tttttctgcg
     6841 ggttttgcca tgctggcgaa aactcagttc cctgatgtga atattctgcg cctggcgttc
     6901 tttggcccat ttattggtgc catcgcgcgg tcggttggtg gtgctatttc cgataaattc
     6961 ggcggcgtgc gggtgacgtt gatcaacttt atttttatgg cgattttcag cgccctgctg
     7021 ttccttacct taccgggcac aggctccggt aatttcatcg cattttacgc cgtatttatg
     7081 gggttgtttc tgaccgcagg tctgggaagt ggttctactt tccagatgat cgccgtcatc
     7141 tttcgccaga taaccattta tcgggtgaag atgaaaggcg gtagtgatga gcaagctcag
     7201 agagaagccg tcaccgaaac ggcggcggct ctgggcttta tctcagccat tggcgcagtg
     7261 ggcggctttt tcattccgca ggcgtttggc atgtcgctca atatgaccgg ctctccggtg
     7321 ggcgcgatga aagtgttttt aatcttctac atcgtttgtg tgctgctgac ctggctggtt
     7381 tatggtcggc ggaagttcag ccaaaaataa gagatcgatg cctgacacgt cttatcaggc
     7441 tacaaccgtg tcgcatccga caaggcatca gatcccaatt cattcctgga gcaggagtta
     7501 tgtcatgagt aaacttttgg atcgctttcg ctactttaaa caaaagggcg aaacctttgc
     7561 cgatggtcac ggacaggtga tgcacagcaa ccgcgactgg gaggacagct atcgccagcg
     7621 ttggcagttc gacaaaatcg tgcgttccac ccacggtgtt aactgtacag gctcctgtag
     7681 ctggaaaatc tacgttaaaa atggtctggt gacctgggaa atccaacaga ccgactaccc
     7741 gcgcactcgc cctgacctgc caaatcatga acctcgcggc tgcccgcgtg gcgcaagtta
     7801 ctcctggtat ctctacagcg ctaaccgcct gaaatacccg ctcattcgta aacgactgat
     7861 tgaactgtgg cgcgaagccc tcaagcaaca cagcgatccg gtactggcgt gggcatcgat
     7921 tatgaacgat ccgcaaaagt gcctgagcta taaacaagtg cgtggacgcg gtggctttat
     7981 ccgctccaac tggcaggagc taaatcagct gattgccgcc gctaacgtct ggaccatcaa
     8041 aacttacggc ccggatcgcg ttgccgggtt ctcgccgatt ccggcgatgt cgatggtttc
     8101 ttacgccgcc ggaacacgct atctgtcgct gttaggtggc acctgtttaa gcttctacga
     8161 ctggtattgc gacctgccgc ccgcctcgcc gatgacctgg ggtgagcaaa ccgacgtgcc
     8221 agaatctgcc gactggtata actccagtta catcatcgcc tgggggtcta acgtaccgca
     8281 gacacgtacg ccggacgccc atttctttac cgaagtacgc tacaaaggca ctaaaaccat
     8341 cgcaattacc cctgactact ctgaagtggc caaattgtgc gaccagtggc tggcaccaaa
     8401 acaaggcact gatagcgccc tggcgatggc aatgggccat gtgattttaa aagagtttca
     8461 tctcgataat cccagcgact actttatcaa ctactgccgc cgctacagcg acatgccgat
     8521 gctggtaatg ctggaacctc gcgacgatgg cagctacgta cccgggcgca tgatccgcgc
     8581 gtctgacctg gtagatggac tgggcgaaag caacaatccg cagtggaaaa ccgtagcagt
     8641 taatacagca ggcgaactgg tggtgccgaa cggttcgatt ggttttcgct ggggagaaaa
     8701 aggcaaatgg aatctggaat ccattgccgc cggtacggaa accgaattgt cgttaactct
     8761 gctcggtcaa catgatgccg ttgcaggcgt ggccttcccc tactttggcg gcattgaaaa
     8821 tccgcatttt cgcagcgtaa aacacaatcc ggtcctggtg cgccaattgc ccgttaaaaa
     8881 cctgacatta gccgatggca acacctgtcc ggtggtcagc gtttatgatt tggtactggc
     8941 gaattacggc ctcgatcgcg ggctggaaga tgaaaacagc gcgaaagatt acgctgaaat
     9001 caaaccgtac accccagcct ggggtgagca aattaccggt gtgccgcgcc agtatattga
     9061 aaccatcgct cgtgaatttg ccgatactgc ccataaaacg catgggcgct cgatgattat
     9121 cctcggcgca ggtgttaacc actggtacca catggacatg aactaccggg gaatgatcaa
     9181 tatgctgatc ttctgcggtt gtgtcgggca aagcggtggc ggatgggcac actatgtcgg
     9241 tcaggaaaaa ctgcgcccac aaactggctg gttgccgctg gcctttgcgc tcgactggaa
     9301 ccgcccaccg cgccagatga acagcacctc gtttttctac aatcattcca gtcaatggcg
     9361 ctatgaaaaa gtctctgctc aggagttact ttcaccgctc gccgatgcca gtaagtacag
     9421 cggtcatctg attgatttca acgttcgtgc cgaacgtatg ggctggctac cttctgcgcc
     9481 gcagctgggg tgtaacccgc tcgggattaa agctgaagcc gacaaggccg gattatcccc
     9541 cacagaattt accgcccagg cgctgaaatc gggcgattta cgcatggcct gcgaacaacc
     9601 agatagcggc agcaatcatc cgcgtaattt gtttgtctgg cgttctaacc tgcttggctc
     9661 ctccggcaaa ggccacgagt atatgcagaa gtatctgctg ggaaccgaaa gtgggattca
     9721 gggtcaggaa ctcggtgcca gcgacgggat caaaccagaa gaagtcgagt ggcaaactgc
     9781 ggcgattgaa ggcaagctcg acctgctggt gacgctcgac ttccgtatgt ccagtacctg
     9841 cctgttctcc gatatcgttc tgcccaccgc cacctggtac gaaaaagacg atatgaacac
     9901 ctcggatatg catccgttta ttcatcccct atctgcggcg gtcgatccgg cgtgggaatc
     9961 acgcagcgac tgggaaatct acaaaggtat tgccaaagcc ttttcgcaag tgtgcgtggg
    10021 ccatcttggc aaagaaaccg acgtggtatt acaaccactg ctgcatgact ctccggcaga
    10081 actctcacag ccgtgtgaag tgctcgactg gcgcaaaggc gaatgcgatc tgattccagg
    10141 taaaaccgcg cccaatattg tggcggtgga acgcgactac cctgctacct atgaacgctt
    10201 tacctcgctc gggccattga tggacaaact tggcaacggc ggtaaaggga tttcgtggaa
    10261 tacccaggat gaaatcgatt tcctcggcaa actcaactac accaagcgtg aaggcccggc
    10321 acaggggcgt ccgctgattg acaccgccat tgacgcatca gaagtgattc tggctcttgc
    10381 accggaaacc aatggtcatg ttgcagtcaa agcgtggcag gcgctgggcg agatcaccgg
    10441 acgcgaacat acccatctgg cgctgcacaa agaggacgag aagattcgct ttcgcgatat
    10501 tcaggcgcag ccacgtaaaa ttatctccag ccccacatgg tctggtctgg aaagcgatca
    10561 cgtctcctat aacgcgggat acaccaacgt tcatgagtta attccgtggc gcacgctgtc
    10621 gggacgccag cagctctatc aggatcatcc gtggatgcgt gcttttggtg aaagcctggt
    10681 ggcttatcgc ccgcctattg acacccgtag cgtcagtgaa atgcgccaga taccgccaaa
    10741 cggcttcccg gaaaaagcac ttaacttcct gacgccgcac cagaaatggg gcattcactc
    10801 aacctacagt gaaaacctgc taatgctgac gctctctcgc ggtggaccaa ttgtctggat
    10861 tagcgaaacc gatgcccgtg aactgaccat tgtcgataac gactgggtgg aagtgtttaa
    10921 cgccaacggc gcgctgacgg cccgcgcggt ggtcagccaa cgtgtaccgc cgggtatgac
    10981 catgatgtat cacgcccagg aacgcattat gaatattcct ggttcggaag taactggcat
    11041 gcgcggcggc attcataact cggtcacccg cgtttgtccg aaaccaacgc atatgattgg
    11101 cggttacgcg cagctggcct ggggctttaa ttactacggc accgtcggct cgaaccgtga
    11161 cgaattcatc atgatccgca agatgaagaa cgttaactgg ctggatgatg aaggtcgcga
    11221 tcaggtacag gaggcgaaaa aatgaaaata cgttcgcaag tcggcatggt gcttaacctc
    11281 gataaatgta tcggctgcca tacctgttcg gtgacctgta aaaacgtctg gaccggacgc
    11341 gaaggcatgg agtacgcatg gtttaacaac gtcgaaacca aaccgggcat tggttatccg
    11401 aaaaactggg aagatcagga agagtggcaa ggcggctggg tgcgtgatgt gaatggcaag
    11461 atccgcccgc gtctgggcag caagatgggc gtaataacca aaatcttcgc caacccggtg
    11521 gtgccgcaga ttgatgatta ctacgaacct ttcaccttcg actacgaaca tttgcataac
    11581 gcaccggaag gcaaacatat ccctactgct cgcccgcgtt cactgattga cggcaaacgg
    11641 atggacaaag tgatctgggg gccaaactgg gaagaattgt tgggcggcga attcgaaaaa
    11701 cgcgcccgtg accgcaactt cgaggccatg caaaaggaga tgtacgggca gtttgaaaac
    11761 accttcatga tgtacctgcc gcgcctgtgc gaacactgcc tcaatcccag ttgcgtggcg
    11821 acctgcccaa gcggcgctat ctacaaacgc gaagaagacg gcattgtgct gatcgatcag
    11881 gataaatgcc gtggctggcg tttgtgcatt agcggttgtc cgtacaaaaa aatctacttc
    11941 aactggaaaa gcggcaagtc agaaaaatgt atcttctgtt acccacgaat tgagtcaggc
    12001 caacccaccg tgtgctcaga aacctgcgtg ggtcgcatcc ggtatctggg cgtgctgctt
    12061 tacgacgccg accgcatcga ggaagcggcg agcaccgagc gcgaagttga cctctatgaa
    12121 cgccagtgcg aagtgttcct cgatccgcac gatccctcag tgatcgaaga agccctgaaa
    12181 caaggtattc cacaaaacgt gattgaagct gcccagcgtt cgccagtcta caaaatggcg
    12241 atggactgga aactggcgtt accgctgcac cctgaatacc gcaccctgcc gatggtctgg
    12301 tacgttcctc cgctgtcacc gattcagtct tacgctgatg cgggcggttt gccgaaaagc
    12361 gaaggcgtgc tgcccgccat cgaaagcctg cgtattccgg tgcaatatct cgccaatatg
    12421 ttgagtgctg gcgataccgg tccggtactg cgggcgctga aacggatgat ggcgatgcgc
    12481 cactatatgc gttcacaaac cgtggaaggc gttaccgata cccgcgccat tgacgaagta
    12541 ggcctgagcg tcgcccaggt cgaagagatg tatcgctacc tcgccattgc caactatgaa
    12601 gaccgttttg tcattccaac cagtcatcgg gaaatggcgg gcgatgcctt cgcagaacgc
    12661 aatggctgcg gttttacctt tggcgacggt tgccacggct cggacagcaa attcaacctg
    12721 ttcaacagta gccgtatcga tgccatcaac atcaccgaag tgcgcgacaa agcggagggc
    12781 gaataatgca gatcctcaaa gtgatcggcc tgttgatgga gtatccggac gagctgttgt
    12841 gggaatgtaa ggaggacgcg ctggcgttga tccgccgcga cgcaccgatg ctcaccgatt
    12901 ttgcactcga cctgctcaac gcgccgctgc tggataaaca ggccgaatgg tgtgaagtgt
    12961 ttgaccgcgg gcgcaccacg tcgctgctgc tgtttgaaca tgttcatgcc gagtcccgcg
    13021 atcgcggcca ggcaatggtg gacctgctgg cggagtatga aaaggtcggt ctgcaactga
    13081 attgtaggga actgcccgat tatctaccgc tgtatctgga gtatttaagc atacttccgg
    13141 acgatcaggc gaaagaagga ttactcaacg ttgcgccgat cctcgccctg cttggcggac
    13201 gcttaaaaca acgcgaggca ccgtggtacg cgttatttga cgccctgttg caactggcag
    13261 gaagccctct atcaagtgac agtgtcacaa aacagattcg cagtgaagag cgtgacgaca
    13321 cccgccaggc gctggatgcg gtgtgggaag aagaacaggt gaagtttatt gaagataacg
    13381 ccacggcgtg tgacagctcg ccgcttaatc aatatcagcg acgctttagc caggatgtcg
    13441 cgccgcagta tgtcgacatc agtgcgggag gtgggaaatg attcagtatc tgaacgtctt
    13501 tttttacgat atctacccgt acttgtgtgg cacggttttt atccttggaa gctggctgcg
    13561 ctacgattac gggcagtata cctggcgtgc cgcctccagc cagatgctgg ataaacgcgg
    13621 gatggtgcta tggtcgaact tgttccatat cggcattctg gggatcttct tcggtcatct
    13681 gttcggcatg ttaacgccgc actggatgta cgcgtggttt ctgccggtgg cggtaaaaca
    13741 gcagatggcg atgatcctcg gcggaatatg cggggtattg acgctgattg gcggtgcggg
    13801 gctactggtg cgtcgtttaa caaatccgcg tatcagggcg acgtcttctg gtgcggatat
    13861 cctgatcctc gccattttgt taattcagtg cattcttggg ttaaccacca ttcctttttc
    13921 agcacaacat ccggacggca gtgaaatgat gaagctggtg ggctgggcgc aaagcattgt
    13981 cactttccag ggcggtgcat ctacgcatct tgatggtgtc gcctttatat tccgcgttca
    14041 tctggtgctt ggaatgacca tcttcctgct cttcccgttc acccgactgg tgcatgtctg
    14101 gagcgcgccg tttgagtact ttactcgtcg gtatcagatt gtgcgttcgc ggcgttaatt
    14161 ctcacgcggg ccggacagcc cttcgctctc cggccacttg caggctatga tagttctcca
    14221 gccacaggag aacattaatg aaaccgcagg tttaccacgt cgatgctttt acctctcaac
    14281 cgtttcgcgg caattctgcc ggtgttgttt tccccgccga taatctcagt gaagcgcaaa
    14341 tgcagcttat cgcccgcgag ttaggtcatt cggaaactgc ttttctgctg cacagcgacg
    14401 acagcgatgt gcgcatccgc tactttacgc caacggttga agttccgatt tgtggtcacg
    14461 cgacagtagc tgcgcactat gtacgtgcaa aggtgttagg tttaggaaat tgcacggtct
    14521 ggcaaacatc gctggcagga aaacaccgcg tgactatcga aaagcataac gatgattatc
    14581 gtatttcgct ggaacaaggt acgccgggct ttgagccacc gctggaaggt gaaacatgtg
    14641 cggcgattat caacgcgctt catctcactg aagacgatat tctgccaggc ttaccaattc
    14701 aggtggcaac cacaggacac tcaaaagtga tgatcccact gaaaccggaa gtggatatcg
    14761 acgccctttc gcctgacctt aatgcgctga ccgctatcag caaacagatt ggctgcaatg
    14821 gtttcttccc gttccagatc cgcccagaca aaaacgaaac cgacggtcgt atgttctcgc
    14881 ctgcgattgg tattgtggaa gatccggtga ccggcaatgc caatggcccg atgggtgcat
    14941 ggttggtaca tcacaacgta ttatcccacg atggcaacgt gttgcgtgtt aaaggccatc
    15001 aggggcgcgc attggggcgt gatggtgtaa ttgatgtgac ggtgacaatt cgagataacc
    15061 aaccggagaa agttaccatt tccggcacgg cggtgattct attccatgct gaatgggcca
    15121 ttgatttttg atgccgttgc ctgatgcgct tcgcttatca ggcctacaac cggactaatg
    15181 taggccggat aaggcgttta cgccacatcc ggcaacctga cataaattat tttcccgcct
    15241 ccgggtgcgt atcaaacgcc gccatcacca gcgctaactc atccacagta aagccatgtt
    15301 tcgcatcatc tacgcccaga ccaaactgtt cctgcatcac ggcatataac gacgccacat
    15361 ctggtagatt tcgctgctca actgcgtgcc cattttcata atgggtaaaa tgaaaattgg
    15421 tcagcgttag cttgccaccg tccggcaaat ggcgacacat cagcaaatga tggcgaaaat
    15481 gcgactgcgg ccagtgcgcc gaccagaaat tgcccatcac ataatcgttt tgttgctgct
    15541 cgcagagatc aaaacggtac atcgactgcc agtgctgatg atgattaaac tgcaacaccc
    15601 aatcatcacc ctcctgtaac aaccgatact ctccgtgtgg cgtgatctgc acgagatcgg
    15661 gaactaaacg aatcggcgcg gttagcgtct gtccaccgaa accgacatca gcaatccatt
    15721 tttccccttc cagttccacc aacaacaaac gatgggtgcg cggcggtaat gctggcggat
    15781 ttgataacac tacgcgccct aacaagctgc gaacgttaaa ccccagctcg cgtaacaccc
    15841 gctcaaacac gccattctgc tcaaaacagt aaccgccacg atgggctgtc accagtttct
    15901 cttccagcga ttgatcatca agctgtattt ccctcggcag caaaacgtcg aggttttcaa
    15961 acggaatagt gcaattgtgt ttcaggtgca atgcacgcag cgtatcaata ttgaccgcag
    16021 cagctcccga ccagttaata cgggcaaaat aatgattcag aatgggcgtc atcaggcgtt
    16081 tcccttagtg gttttctcaa cttataacct ggaatagacg ccgcaccggc tattttgtgc
    16141 gatcgcggac atgcatcacg ttcgcaaatc gccaatcccg ttaatttcgt tacttattgc
    16201 gttacgagct acgcttttaa aacacccttc atagcgaggc gatatcgtga gccaattcat
    16261 tcccgtcgaa ttacaccatg ccagccgtct gttaaatcat ggcccaactg tcttgatcac
    16321 cagtttcgat gagcaatccc agcggcgtaa cattatggct gcagcctggt caatgccggt
    16381 ggagtttgaa cctccacgcg tggcgattgt ggtagataag tcgacatgga ccagggagtt
    16441 gattgagcgt aacggtaaat ttggcatcgt tatcccaggc gttgcagcga ctaactggac
    16501 gtgggcagtg ggaagtgtat ctgggcgtga agaggataaa ttttattgct atggcattcc
    16561 ggtggtgaaa ggtccggtgc ttggtttgcc acttgttgaa gagaaatgtc tggcgtggat
    16621 ggagtgccga ttgctaccag cgacttccgc gcaagagaaa tacgacacgc tgtttggcga
    16681 agtggtatca gcagcggcag acgcacgggt atttgtcgaa ggccgctggc agtttgatga
    16741 tgataagctc aatacgttgc atcatttagg cgctggcacg tttgttacca gcggcaagcg
    16801 tgttacggca ggttaagcat tacatgctgt aaccgggttt ctttatcaaa gcctccattt
    16861 ggggctcgat ttcggcatcc cagatagctt gccaagattc tggttgaatc tgctgtagag
    16921 aaatgcttat cgagctatct ttactgttca gatggcgaat aataacgtcg gtaatatctg
    16981 cagcaagtgc tgctttttgt tgttcgtcca gttcacgcgg aaaacattta atgtctatgt
    17041 gcggcatgat tgtgctcctt ttgaaagatg tcagtaaaat taacacacgt tgtgaatgtc
    17101 gtgtgtccgg taatgcgttt ttacggcaat aatgcctact tgaaattgta catatttgta
    17161 ctacacactt ttaaataaag cgccaatgaa aacgttaact tgcagaatat attcttcgcc
    17221 aacatgccat taagaaaaca tcaagttccc ggcttgccaa tttgttaacg cccattatag
    17281 ttattcttgc cctcactaaa aattttcatc cgtaattacc cccaataaaa atccattctg
    17341 gagacgctta agatgcagct taatcatatc gttaaaagtc tgctaataac gggattattc
    17401 accaccagtt ctttacctct cctcgcggcg gaagctccta aagacgccac cgcagccaca
    17461 caacaagcaa acaatttact ctacaaccag ttgccgtttg ccgataacac tgactttact
    17521 gatgcccata aaggttttat tgcccctatc cctcaagacg tgattaaggg cgaacaaggg
    17581 aatgttatct gggatccaca acattacgct tttatcaaag aaggcgataa agcccctgat
    17641 acggtgaatc cgagtttatg gcgtcaggcg caactgataa atatcagcgg gttattcgaa
    17701 gtaactgaag gtgtctatca gattcgcaat ctcgatctat caaatatgac catcattgaa
    17761 ggcaaagagg gtattaccgt cgttgatccc cttgtttcag cagaaacagc caaagtgggt
    17821 atggatctct attacaaaaa tcgcggcaaa aaaccggtag tggcggttat ctatacacac
    17881 agtcatgtcg atcactacgg tggtgttcgt ggcgtaattg atgaagctga cgttaaatca
    17941 ggcaaagtta aaatctatgc gccagccgga tttttggagg aagccgtttc cgaaaacatc
    18001 atggctggca atgttatgag ccgacgagcc agttatatgt atggcaactt attgaaacct
    18061 gatgttaaag gccaggtggg ggctggactg ggaacgacta cttcagcagg aacagtcacg
    18121 ttgattgctc ctactaacta catcaccaaa accggacaaa tagaaactat tgacgggttg
    18181 acttatgatt tcctgatggc gccaggttct gaagcgcctt ctgaaatgct gtggtttatt
    18241 gaagaaaaga aactgatcga aaccgctgaa gatgtcacgc acacccttca taacacctat
    18301 tcgctgcgtg gggctaaaat tcgccaaccg ctgccgtggt caaaatatat taatgaagct
    18361 ctcaatttgt ggggtgataa agccgagatt attctcgctc aacatcactg gccaacctgg
    18421 ggcaacgata acgtggtcaa actgcttaaa agccagcgtg atttgtatcg ttacatcaat
    18481 gatcaaactc tgcgcatggc aaatcaaggg atgacccgtg atgaaattgc cgcaaacttt
    18541 aaacttccct cttcactggc taacacctgg gcgaatcgcg gctattacgg ttcggtaagt
    18601 catgacgtga aagcaactta cgtgctttat ctcggctggt ttgatggtaa ccccgcgacg
    18661 cttgatgaac tgccgccaga agaaggcgca aaaaaattcg tcgagtatat gggcggtgct
    18721 gatgccattt tacagaaagc caaacaggat tatgaccagg gtaatttccg ctgggttgcc
    18781 caagttgtta gtaaggtggt gtttgccgat ccaaacaacc aggcagcgag agatctggaa
    18841 gctgatgcgc ttgagcagtt aggctatcag gctgaatctg ggccgtggcg taatttctat
    18901 ctcactggcg cacaggaatt gcgtaatggt gtacaaaaat taccaacacc aaatactgcc
    18961 agcccagaca ccgtgcgtgc gatgacgccg gagatgttct ttgattatct tgccgtccat
    19021 attaatggtg agaaagcggc agatgccaaa accgtgttga atttcgattt tggtgaagat
    19081 ggcggcacct ataaagtgga gcttgaaaat ggtgttctca atcatacagc tggtgtagag
    19141 gcttcggatg ctgatgccac tatcactctg tctcgtgatg tattgaacaa aattgtactg
    19201 aaagaagaga cgctgaaaga agccacggcc aaaggagatg tcaaaattac tggcaatgcg
    19261 gaaaaactca acgagctgtt aggttatatg gataattttg aattttggtt caatatagtg
    19321 acaccataaa tttactgcat accgggcagg tatcctgccc ttttttggtt aaaaaatggc
    19381 tgaacgaaga aaaacgcatc actcatggaa aacccgccgc gatatggtta ttcgcggcat
    19441 gacgattagt ttgccatggt ttgcttttat caatgtcagt tttgcattga taattttgct
    19501 gcgccgtgtt ctgtttaatg accttactcc accctggtta aatgaaaaaa gccttataca
    19561 ttctattgat atatctgcaa cgggtattct gctaatttgc agtggtttac tactcatccc
    19621 tcggcaaaaa acattaccta tccaggtgct attagttgcc ctgagcctgc tatggtcatg
    19681 gtgtagttat cactttattg cgtactggac gctgcagttt gcttatccac tatgtgtatt
    19741 attaatgctt agtggcgttg tcgcacttta tttccatacc ccatcattgc tcgcatttgt
    19801 cattccctta tggttcacca ctccaatagc gagtcttatg cttaatcaac agatcaatat
    19861 tcattttgct gttgtatggt gtattttttc actggcgcta tatggtgggc gactcatttt
    19921 gttacgttgg tttgaagaag catgggtaca aaacagttat aacaaccagt taattaatcg
    19981 ccttgatgca ttagcacatc gtgatccttt aactgggata gcaaaccgac gtgcaatgaa
    20041 cagtatctta cacgacgcga tagataacgg cgggtcattt gctcttatta tgctggatgt
    20101 cgattttttt aaacgttaca acgacactta cgggcatcct gctggggatc gttgtctaat
    20161 acaagttgcc gacgctctac agcgttcaac tcgccagtcg gaggatgttg ttgtccgtta
    20221 tggtggcgaa gagtttgcca tcatactttt taatgcaaca ttgccggaag cagagacggt
    20281 agcagccaga gtgaagcaag aacttaaact ggcagctatc ccacatcagg catctgctgt
    20341 taatgcattc gttacggtta gtcaggggat tacctgttct gctcccgcaa aaacggctga
    20401 acaaattatt tcagatgccg acgctgcgtt ataccgggcg aaagaaagtg ggcgaaatcg
    20461 atgggcttta taagcattag gggactggac agattacgtc tctcctcgct taacaagggc
    20521 tgacggaggg taataattgt ctatagctat cttcttagcg atgtctttct ggtatcacat
    20581 tggatcggtg caattatttt tcctttaata cccattatgt taagaaattc agaaacagct
    20641 gtaatctcat tggttttttc attcgtcttg atcttccaag gaatctgact gtgcattatt
    20701 gagaacacac taataacgtg aatcgctccc ttgcgacgac tcttgtcata agagtaccgg
    20761 agcgtttttc catcaattgc aatgacgtct ttatcatctg aagaatggca gtcaagcatc
    20821 cagttaataa agcactcatg aaattttgca ggactgatac aggatacaac tctggcaatg
    20881 gtatcgtgaa caggaatacc attatcaaaa tcaccatatt gcttcaaaaa atcgagatgt
    20941 gtttccccaa aatcctctat atcttcccag ccttctgcac ccgaaataac ggcacaaata
    21001 gtcaacagta gaatatccga taatttatgt tccactttcc aggcttgtct
//
LOCUS       DAPHCV010000090        20652 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.090, whole genome shotgun sequence.
ACCESSION   DAPHCV010000090 DAPHCV010000000
VERSION     DAPHCV010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.090"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..261
                     /locus_tag="RH033_003346"
     CDS             <1..261
                     /locus_tag="RH033_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HDV2992486.1"
                     /translation="LTYRYGDEHQPVTTADILTPRRREDYGKDLWSAYQTIQENMLKG
                     GISGRSARGKRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            343..822
                     /locus_tag="RH033_003347"
     CDS             343..822
                     /locus_tag="RH033_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDV2992487.1"
                     /translation="MKTVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPQWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFSAMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            834..1310
                     /locus_tag="RH033_003348"
     CDS             834..1310
                     /locus_tag="RH033_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HDV2992488.1"
                     /translation="MQQLSFLPGAMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            1373..1594
                     /locus_tag="RH033_003349"
     CDS             1373..1594
                     /locus_tag="RH033_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HDV2992489.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAETYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            1757..2125
                     /gene="cbtA"
                     /locus_tag="RH033_003350"
     CDS             1757..2125
                     /gene="cbtA"
                     /locus_tag="RH033_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HDV2992490.1"
                     /translation="MSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLGSGGYVYLAVYPTPETKK"
     gene            2215..2589
                     /locus_tag="RH033_003351"
     CDS             2215..2589
                     /locus_tag="RH033_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDV2992491.1"
                     /translation="MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEKR"
     gene            2586..3074
                     /locus_tag="RH033_003352"
     CDS             2586..3074
                     /locus_tag="RH033_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992492.1"
                     /translation="MKLSLMLEADRINVQALNMGQIVVDVDGVNLTELINKVAENGYS
                     LRVVEESDQQSTCTLPPFATLAGIRCSTAHITEKDNAWLYSLSHQTSDVGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVITLTRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            3086..3283
                     /locus_tag="RH033_003353"
     CDS             3086..3283
                     /locus_tag="RH033_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992493.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNNEDKTDSAALLPC
                     IEQAREDIRTLRQLQLQHQNR"
     gene            3380..3949
                     /locus_tag="RH033_003354"
     CDS             3380..3949
                     /locus_tag="RH033_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDV2992494.1"
                     /translation="MSEITVSRPEVVNENTDVICSTSVRSRSLEYDNFPEISEANILS
                     TFEQLHQNKDEVFERGVINVFKGLSWDYKTNSPCKFGSKIIVNNLVRWDQWGFHLISG
                     MQADRLADLERMLHLLSGKPIPDNRGNITINLDDHIQSVQGKGRYEDEMFIIKYFKKG
                     SAHITFKRLELIDRINDIIARHFPSVLSA"
     gene            complement(4229..4765)
                     /gene="yghD"
                     /locus_tag="RH033_003355"
     CDS             complement(4229..4765)
                     /gene="yghD"
                     /locus_tag="RH033_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HDV2992495.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLTQHLAGRSPREKGMLLAAA
                     VFLFSAGYYVLIWQPLSERIEQQETMLQQMVAMNARLKSAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIDPVVFNDLLNWLKALDEKYALRVTQIDVS
                     AGEKPGMVNVQRLEFGRG"
     gene            complement(4767..5945)
                     /gene="gspL"
                     /locus_tag="RH033_003356"
     CDS             complement(4767..5945)
                     /gene="gspL"
                     /locus_tag="RH033_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HDV2992496.1"
                     /translation="MSSMLEIFFPLCAADPIRWQRHTPDVEHGIWPDVADERLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVPRKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQANGIARATVAPDWMSIPCGFMAGDAQRVICRIDECRGWS
                     AGRALAPVMFRAQLNEQNLPISLTVVGIAPEELSAWAGADAERLTVTALPAITTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALVVERGVTLWSVSEQVAQSRTQA
                     EKQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTDLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALAVDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(5942..6919)
                     /gene="gspK"
                     /locus_tag="RH033_003357"
     CDS             complement(5942..6919)
                     /gene="gspK"
                     /locus_tag="RH033_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HDV2992497.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPIAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTILSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(6916..7521)
                     /gene="gspJ"
                     /locus_tag="RH033_003358"
     CDS             complement(6916..7521)
                     /gene="gspJ"
                     /locus_tag="RH033_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HDV2992498.1"
                     /translation="MRRTRAGFTLLEILVAIAIFASLALMAQQVTNGVARVNSAVADH
                     DQKLNLMQQTMSFLTHDLTQIMPRPVRSDQGQREPALLAGPGVLASESGGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQLS"
     gene            complement(7518..7889)
                     /gene="gspI"
                     /locus_tag="RH033_003359"
     CDS             complement(7518..7889)
                     /gene="gspI"
                     /locus_tag="RH033_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HDV2992499.1"
                     /translation="MKRGFTLLEVMLALAIFALAAMAVLQIASGALSNQQILEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTTPLSTGNALLQAVDIEVSLH
                     EDFSPVIQSRRAWFSAVGGQQ"
     gene            complement(7886..8449)
                     /gene="gspH"
                     /locus_tag="RH033_003360"
     CDS             complement(7886..8449)
                     /gene="gspH"
                     /locus_tag="RH033_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HDV2992500.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSESPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDPPGYQFMQRRQGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLAHNGVLSLNQCDERMP"
     gene            complement(8453..8908)
                     /gene="gspG"
                     /locus_tag="RH033_003361"
     CDS             complement(8453..8908)
                     /gene="gspG"
                     /locus_tag="RH033_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HDV2992501.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(8925..10148)
                     /gene="gspF"
                     /locus_tag="RH033_003362"
     CDS             complement(8925..10148)
                     /gene="gspF"
                     /locus_tag="RH033_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HDV2992502.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKDLIPVHIEA
                     RMNASAGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRMLIAM
                     SDTLQTSGVYWLAGLLGLLVLGQRLLKNPAMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(10148..11641)
                     /gene="gspE"
                     /locus_tag="RH033_003363"
     CDS             complement(10148..11641)
                     /gene="gspE"
                     /locus_tag="RH033_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HDV2992503.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFGLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSLELTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQSVPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(11641..13701)
                     /gene="gspD"
                     /locus_tag="RH033_003364"
     CDS             complement(11641..13701)
                     /gene="gspD"
                     /locus_tag="RH033_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HDV2992504.1"
                     /translation="MFWRDMTLSIWRKKTTGLKTKKRLLPLMLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWGSKDAGLM
                     QFANGTQIPIGTLGAAISAAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLTLMPHTAQPILPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(13731..14690)
                     /gene="gspC"
                     /locus_tag="RH033_003365"
     CDS             complement(13731..14690)
                     /gene="gspC"
                     /locus_tag="RH033_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HDV2992505.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHILSQRQSLNTDKEHLRKMVRGL
                     FWLMLLIISAKMAYSLWRYFSFSAEYTVVSSSANKPLRADAKPFDKNDVQLVSQQNWF
                     GKYQPVAAPVKQPESAPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGERLGSH
                     NAVIEEINRDHVMLRYQGKMERLSLAEEERPPVAVTSKKAVSDEAKQAVAEPAASAPV
                     EIPAAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFREGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARYDISIALR"
     gene            complement(14708..15118)
                     /gene="gspS2"
                     /locus_tag="RH033_003366"
     CDS             complement(14708..15118)
                     /gene="gspS2"
                     /locus_tag="RH033_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HDV2992506.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISESGTQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(15184..15993)
                     /gene="pppA"
                     /locus_tag="RH033_003367"
     CDS             complement(15184..15993)
                     /gene="pppA"
                     /locus_tag="RH033_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HDV2992507.1"
                     /translation="MLFDVFQQYPAAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGETPSTQSKISLALPRSHCPHCQQTIRVRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASVIDLDNQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGW
                     VGALSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATLYLQALF"
     gene            complement(16157..>20652)
                     /gene="sslE"
                     /locus_tag="RH033_003368"
     CDS             complement(16157..>20652)
                     /gene="sslE"
                     /locus_tag="RH033_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HDV2992508.1"
                     /translation="AGCDGGGSGSSSDTPPVDSGTGSLPEVKPDPTPNPEPTPEPTPD
                     PEPTPEPTPDPEPEPEPEPVPTKTGYLTLGGSQRVTGATCNGESSDGFTFTPGEDVTC
                     VAGNTTIATFNTQSEAARSLRAVEKVSFSLEDAQELAASDDKKSNALSLVTSMNSCPA
                     DTEQVCLEFSSVIESKRFDSLYKQIDLAPEEFKKLVNEEVENNAATDKAPSTHTSPVV
                     PVTTPGTKPDLNASFVSANAEQFYQYQPTEIILSEGRLVDSMGNGVVGVNYYTSSGRG
                     VTGENGKFNFSWGETISFGIDTFELGSVRGNKSTIALTELGDEVRGANIDQLIHRYST
                     TGQNNTRVVPDDVRKVFAEYPNVINEIINLSLSNGATLDEGEQVVNLPNEFIEQFKTG
                     QAKEIDTAICAKTDGCNEARWFSLTTRNVNDGQIQGVINKLWGVDTSYKSVSKFHVFH
                     DSTNFYGSTGNARGQAVVNISNSAFPILMARNDKNYWLAFGEKRAWDKNELAYITEAP
                     SIVQPENVTRDTATFNLPFISLGQVGEGKLMVIGNPHYNSILRCPNGYSWGGGVNSKG
                     ECTLSGDSDDMKHFMQNVLRYLSNDIWQPNTKSIMTVGTNLENVYFKKAGQVLGNSAP
                     FAFHEDFTGITVKQLTSYGDLNPEEIPLLILNGFEYVTQWSGDPYAVPLRADTSKPKL
                     TQQDVTDLIAYLNKGGSVLIMENVMSNLKEESASSFVRLLDAAGLSMALNKSVVNNDP
                     QGYPDRVRQRRATGIWVYERYPAADGAQPPYTIDPNTGEVTWKYQQDNKPDDKPKLEV
                     ASWQEEVEGKQVTRYAFIDEAEYTTEESLEAAKAKIFEKFPGLQECKDSTYHYEINCL
                     ERRPGTDVPVTGGMYVPRYTQLNLDADTAKAMVQAADLGTNIQRLYQHELYFRTKGSK
                     GERLNSVDLERLYQNMSVWLWNDTKYRYEEGKEDELGFKTFTEFLNCYANDAYAGGTK
                     CSADLKKSLVDNNMIYGDGSSKAGMMNPSYPLNYMEKPLTRLMLGRSWWDLNIKVDVE
                     KYPGSVSAKGESVTENISLYSNPTKWFAGNMQSTGLWAPAQQDVTIKSSASVPVTVTV
                     ALADDLTGREKHEVALNRPPRVTKTYTLEANGEVTFKVPYGGLIYIKGDSKDDVSANF
                     TFTGVVKAPFYKDGEWKNDLDSPAPLGELESASFVYTTPKKNLEASNFTGGVAEFAKD
                     LDTFASSMNDFYGRNDEDGKHRMFTYKNLTGHKHRFTNDVQISIGDAHSGYPVMNSSF
                     STNSTTLPTTPLNDWLIWHEVGHNAAETPLTVPGATEVANNVLALYMQDRYLGKMNRV
                     ADDITVAPEYLEESNGQAWARGGAGDRLLMYAQLKEWAEENFDITKWYPDGKLPAFYS
                     EREGMKGWNLFQLMHRKARGDDVGNSTFGGKNYCAESNGNAADTLMLCASWVAQTDLS
                     EFFKKWNPGASAYQLPGATEMSFQGGVSSSAYSTLASLKLPKPEKGPETINKVTEHKM
                     SAE"
BASE COUNT         4666 a         5880 c         5188 g         4918 t
ORIGIN      
        1 ctgacttacc gttatggtga cgaacatcag cccgtcacca ccgccgacat tctgacacca
       61 cgacgccggg aggattacgg taaggacctg tggagtgctt atcagaccat ccaggagaat
      121 atgctgaaag gcgggatttc cgggcgcagt gccagaggaa aacgtatcca tacccgggcc
      181 attcacagca ttgataccga cattaagctc aaccgcgcat tgtgggtgat ggcagaaacg
      241 atgctggaga gcctgcgctg atgccgtttc cctgagtgag ggaatcattc agctttcgtt
      301 cctgaaaaac acacatttat ttgttattta aggagttatc tcatgaagac cgtttctcag
      361 aataccccca caatttattc agctacaaca ccagagaata atccgcctca gctggttgcc
      421 agcctcgtcc ctgatgaaca gcgcatcagc ttctggccgc agcattttgg cctcattcca
      481 cagtgggtga ccctggagcc ccgtatcttc ggctggatgg accgtctgtg tgaagactac
      541 tgcgggggta tctggaatct gtacaccctg aacaacggtg gcgcatttat ggcacccgaa
      601 ccggatgacg atgatgacga aacatgggta ctgttcagtg ccatgaacgg taaccgcgct
      661 gaaatgagcc cggaagccgc cggcattgct gcctgtctga tgacgtacag tcatcacgcc
      721 tgtcgtacgg agtgttatgc catgacggtc cattattacc ggctgcggga ttacgccctg
      781 cagcatccgg aatgcagcgc cattatgcgc atcattgact gaggagagag gccatgcaac
      841 agctttcctt tctgcccgga gcgatgacgc ccggcgagcg cagccttatt cagcgggccc
      901 tgaaaaccct ggaccgtcat cttcatgaac ccggcgtggc cttcacctcc acccgtgcgg
      961 cacgggaatg gctgattctg aacatggcgg gactggagcg tgaagaattc cgggtactgt
     1021 atctgaataa ccagaatcag ctgattgccg gtgaaaccct cttcaccggc accatcaacc
     1081 gcacggaagt ccatccccgg gaagtgatta aacgcgccct gtaccacaat gccgctgccg
     1141 tggtgctggc gcacaatcac ccgtccggtg aagtcacacc cagtaaggca gaccggctta
     1201 tcaccgaacg tctggtacag gcactgggcc tggtggatat ccgggtgccg gaccatctga
     1261 tagtcggtgg caaccaggtt ttctcctttg ccgaacatgg tctgctttaa cccgtcacaa
     1321 ccacatcaca cctgttttca cttttatctt ctgtcttcag aggtatccca ttatgaaaat
     1381 tatcactcgt ggtgaagcca tgcgtattca ccaacaacat cctgcatccc gtctttttcc
     1441 gttctgcacc ggtaagtacc gctggcacgg cagtgctgag acgtataccg gtcgtgaagt
     1501 acaggatatt cccggtgtgc tggccgtgtt tgctgaacgt cgtaaggaca gttttggccc
     1561 gtatgtccgg ctgatgagcg tcaccctgaa ctgatgttcc gtcacccgga aaacgagtac
     1621 gactggctga gctcagacgc cgctgtggat gaggccctgc tcataaacga atacaccttt
     1681 accgaaggcg gtctgcgcgc cggctgacct gtcatcgtta aaccattctt aaccgatttt
     1741 ctgtgaggat tttatcgtgt cagacgcact ctccgggaca atgcttcccg acgacaatca
     1801 cgaccgcccc tggtgggggc tgccctgcac cgtgacgccc tgtttcgggg cacgtctggt
     1861 gcaggagggt aaccgcctgc attaccttgc cgaccgcgcc ggtatcagag gcctgttcag
     1921 cgatgcagat gcgtaccacc tggaccaggc ctttccgctg ctgatgaaac aactggaact
     1981 catgctcacc agcggtgaac tgaatccccg ccatcagcat accgtcacgc tgtatgcaaa
     2041 aggtctgacc tgcaaagctg acactctcgg tagcggtggt tacgtttatc tggctgttta
     2101 tccgacgccc gaaacgaaaa agtaaaccac cggaataacc ttctgctccg gcctggtgct
     2161 ttcaccacgc cacttttcca tttttcatct ctgcatatca ggaaaacctt cagtatgaaa
     2221 acattacccg atacacacgt acgggaggca tcgagctgcc cgtctcccgt caccatctgg
     2281 cagactctgc tctcccgact actgggccag cattacggcc ttacactgaa tgacacaccg
     2341 ttcgccgatg aacgtgtgat tgagcagcat attgaagctg gcatttcact gtgtgatgcg
     2401 gtgaattttc tcgtggaaaa atacgcgctg gtgcgtaccg accagccggg attcagcact
     2461 tgtccccgct ctcagttaat aaacagtatt gatatcctcc gggctcgccg ggcgaccggc
     2521 ctgatgaccc gcgacaatta cagaacggta aataacatta ccctgggtaa gcatccggag
     2581 aaacgatgaa actttccctg atgctggaag ccgacagaat taatgtgcag gcactgaaca
     2641 tggggcaaat tgtcgttgac gtcgatggtg ttaatctcac tgaactgatt aacaaggtcg
     2701 ctgaaaacgg ttattcactc cgcgtggtgg aggaatccga ccaacagtca acctgcacac
     2761 taccaccgtt tgcaaccctt gccggcatac gctgcagtac cgcacatatc acggaaaagg
     2821 ataacgcctg gctgtactcg ctgtcgcatc agaccagtga cgttggtgaa tcagaatgga
     2881 ttcatttcac aggtagcgga tatctgttac gtaccgatgc gtggtcatat ccggttctgc
     2941 ggcttaaacg cctggggctg tcaaaaacgt tccgtcgtct ggttatcaca cttacccgac
     3001 gttatggcgt cagtctcatt catctggatg ccagcgctga atgcctgccg ggtttaccca
     3061 ctttcaactg gtaaccagga acaacatgaa atcattaacc acggaaaccg cactggatat
     3121 tctgattgcg tggctgcagg acaatatcga ctgcgaatcg ggaattatct ttgacaacaa
     3181 tgaggataaa acggattcag cagcactgtt gccctgtatc gaacaggcca gagaggatat
     3241 ccgtaccctg cgccaactgc agcttcagca ccagaaccgg tgagtctcac tcatcatctc
     3301 actcaccaga cttcattcca ctgacgccag cctgaacacg gctggcgttt tcatttatct
     3361 gcaaaaagga atatcgatta tgtctgaaat cacagtctcc cgtccggaag tggtcaacga
     3421 gaatacggac gttatctgct ccacctcagt caggtccagg tcactggaat atgataattt
     3481 tccggaaatc agcgaagcga acattctgag cacatttgaa caactgcacc agaacaaaga
     3541 tgaagtgttt gaacggggag tgatcaacgt cttcaaaggg ctgagctggg attacaaaac
     3601 caactcaccc tgtaaatttg gcagtaaaat tatcgtcaac aatctggtga gatgggacca
     3661 gtggggattt catcttatca gtggaatgca ggcagatcgc ctggctgacc tggaaagaat
     3721 gttgcatctg ctcagcggta aaccgatccc cgacaaccga gggaatatca ccattaatct
     3781 ggatgaccac atacagtccg ttcagggtaa aggacgctat gaagatgaga tgttcatcat
     3841 taaatacttt aagaagggat ctgcacacat cactttcaaa aggctggagc tgattgacag
     3901 aattaacgat ataatagcca ggcactttcc ttctgtgctc tcagcctgac cccgagtttg
     3961 attccctttc gatatcaaaa gggactgcgg gtacaaaaga gggtacatat ttcaccaaac
     4021 caaacaaaat aaactaatat caacatgata gaagcactct tcgattccga gtccggcacc
     4081 actaattctt aagaacccgc cacaaggcgg gtttttgctt ttggctctga caataacctt
     4141 cacgaaaaaa attagcttat aaagtctggg ggaattactc tcgccacgtt aacgagagta
     4201 attttattga tattaatctc ctgatacttt atccccgtcc aaattccagc cgctgcacat
     4261 tcaccattcc aggcttctca cctgcgctga catcaatttg tgtcacccgc agcgcatact
     4321 tttcatccag tgcttttaac cagttcagca ggtcattaaa caccaccgga tctatccaga
     4381 cctgaatatt ctccccgcgc tcggctatcc gcttgataac cactgagtgc gcagaagcac
     4441 tatcgctgat gacccgcgat acctgtgcag gcgttgttgt ggcggatttt cgcgccgcaa
     4501 taatatccgg cgcggcgctc ttcagtcgcg cgttcatcgc caccatctgt tgcaacatcg
     4561 tctcctgttg ctcaatccgt tcgctcaaag gctgccagat gaggacgtaa tatccggcgc
     4621 taaacagaaa caccgccgct gccagtaaca tgcccttttc acgcggcgaa cgccccgcca
     4681 ggtgttgtgt cagccagtgt tcgccacggc ttaactggcg ttcacgccat tgctgaaaat
     4741 agtgaataaa tttatcgcgt aacatgttat ttcctccgca acgttacgcc gccggaaacc
     4801 gcatcaccct ctttctgtaa cgcgtcctgt tgcacaacat aatctaccgc cagtgcgcta
     4861 cgcagtttgt cgaagctggc aaagttcgca gcccgtagct ggaggtgaag cgtctggcgt
     4921 ttttgatcaa aggtgaaacc acgcatctca atgtcggaaa gtgatgcgga tttcagggta
     4981 ctggcgatcg cagacaactc ggcaagcaga tcggtatcat cagcctgtgg gcgatatttt
     5041 ttcagcgcca tcgtcacctg cgagcgtaaa ttcacaatcc gcttctgctc cgggaatagc
     5101 gttaagaact gtttctccgc ctgggtgcgg ctttgcgcca cctgttcgct gacgctccat
     5161 aacgtcacgc cccgttccac taccagcgca accagaatca gcaatatcgg cagaatcatc
     5221 acccgccagc gcgcccactg ttttcggtag ctaacacgag gctgccacgg ccctgttagc
     5281 aggttccctt ccggttcgcc ataagtggta atggcgggca gagccgtaac ggtcaggcgt
     5341 tcggcgtccg caccagccca tgcagacagt tcttccggtg caatgccgac cacagttagt
     5401 gaaatcggta aattctgctc attgagctgt gcgcggaaca tgaccggagc cagcgcccgc
     5461 ccggcgctcc atccccggca ttcatcgata cggcagataa cccgttgcgc atcgccagcc
     5521 ataaacccac aaggaatgga catccagtcc ggcgcgacgg tagcgcgggc gatgccgttt
     5581 gcctgcaacc actgcgcaat gttgcgcata tgctgctggt gaatcacagc tacggttgcc
     5641 agttgctggt cgattttcaa cggggcgaaa tgcagttcat cgatatcctg gttcagctct
     5701 tcttccagca aggcgggcag aatcgtcggt atctgcttgc ggggcacatc aggcagttca
     5761 acctgccaga cgctgatcca ttcgccggga atgtagagtc gaatcgcatc agtttgcagc
     5821 cattgctgga gacgttcgtc agcaacgtca ggccagatac cgtgctccac gtcgggtgta
     5881 tggcgctgcc aacggatggg atcagcggcg caaagcggga aaaaaatctc aagcatggaa
     5941 ctcactcact ttctcctgtc tgatgccaga gaacagaaaa gtgttgtggg cccatgcgga
     6001 caattaacga attcatcgtc agttcaatct cattcacggt gatatctgaa cgcagccaga
     6061 agtaattgct gtccacgctc aggatggttt ttagctgttt tttagtacgc tcatcgacgt
     6121 ctgcaagtag cggctgagca agaaactgat cgacatcttc ccagcccttc gccggacgtt
     6181 gttgtaataa cgcccgcgcc tgaacagggc ttaaccacgg gtcaaacagc gcctcaagaa
     6241 tcacactttg cgtgacgtct aaggtattga tgttgatttg ctggcgggtc atcggcagcg
     6301 cacagaccag cggtttcagt ttttgataaa gtccggcgtc cattccctgt accacgcgca
     6361 tctcactgat atcggccagc ggttgattgg cggcgtagaa cggcaccgag cgggcgagat
     6421 actcgctgtc ttcacgcccc agacgcgtct gcacgctgcg gtcctcgtca ataaactccc
     6481 acaggctttc ggctatcagt tcggcccgat aagcaggcac atccaggcgc gagatcaggg
     6541 caatcagttg ttgtaccgcg ataggacgcg acgccgttgt cggctgagcg agggcattca
     6601 ggttaaagca ggcctgggcg tcacgcaggg tgacggcaat ttgcccctgc ggcagtggga
     6661 aaaaacgagg gccggaagcc cacggctgcg ccagatgcac gcgcttttca tttttcaggc
     6721 tcagactgag tgcgctcaac gccaggcttt ccgcactggc gctgtaccac agcgcctgct
     6781 ggtactcctg ctggctgcgc gttcgcccaa gttgttgctg cattcgcccg gaaagcgtga
     6841 tggtcaccag catcataacc gccagcaata ccagcaccac gaccagtgcc attccgcgtt
     6901 ttggtggtga ggtgatcatg ataattgcgg cccgcgtaac aaccagatgc gttcaatctc
     6961 gccccattgc ggcgaatgca gggtcatgcg cactgccaca gggatcgcct gtactgacga
     7021 ccagctctcc tgccagcgcg tgccgtcgta gaactgcaag cggagcgaat ccgccggaat
     7081 caacttttgc attgtcggct tcacgctgcc tgctgcgtcg gtcagcggcc aggctaaccg
     7141 ttcgagatag ccgtcatgaa tgcggtaacc gacggtgagc agattgctgc gtggcagacg
     7201 catcaacggg ttaaccacac cgccgcgcac aaaacgcatt cctccactct cagacgccag
     7261 cacgccaggg ccagccagta acgctggttc acgctggccc tgatcgcttc ttaccggacg
     7321 cggcattatt tgggtcagat cgtgggtcag aaaactcatc gtttgctgca tgaggtttag
     7381 tttttgatcg tgatcggcga cggcgctatt cacgcgtgcg acgccgttag tcacctgctg
     7441 cgccatcagt gccagcgagg caaaaatggc tatcgccacc agtatttcca gtaacgtgaa
     7501 accagcgcga gtccttctca ctgttggcct cccacggcgc taaaccaggc gcgtcgtgac
     7561 tgaatcaccg gcgaaaagtc ttcgtgaagg ctgacttcaa tatccaccgc ctggagcagc
     7621 gcgttgcctg tactcagcgg tgtggttcgc cagtaccagc ggcttcctgc catatcactc
     7681 tcgccctggt gcctgaccgc tcgttgttca cgggtcatca ggtaaagcag cgcggtctgg
     7741 ttttcagcta cccagcccgc taccgttttc tcctcaagaa tctgctgatt actcagcgca
     7801 ccgctggcaa tctgtaacac cgccatggcg gccaacgcaa aaatcgccag cgcgagcatc
     7861 acttccagca aggtaaatcc gcgcttcatg gcatcctctc atcacattga ttgagggata
     7921 acacgccatt gtgcgccagc tttaccgccc aacatgcgtt ttgcgccgcc gagtagaagc
     7981 gcagcgtaaa cggcgtggcg ggttcaaaag gcgaaaaacg gatctgcggt gtcttctttt
     8041 tcgcctcttt ttgcagctcc agttcaatat cgtgcagcgt caggcggcga cgctgcagct
     8101 ccagcgcata ttccttctgc cagatatcac ttccgggttg taacagcatc tgcacctgtt
     8161 tcggcaccgt aacctgtgcc gataaccggg tcgcagaaac gggcaaccac tgcccctgac
     8221 gacgctgcat aaactggtat cccggcgggt cgattagtac accgagtgtc tgcccttcga
     8281 tcactgccct gtctttaaac tgcgcaaagc gagtcagaaa atcctgcgcc gcttttttcg
     8341 cgggcgattc agaatcggtc gcaaacgtct gtaccacgcc cgcactggca aggccgataa
     8401 ggaaaatcac cagcatgatt tccagaagag tgaatccgcg ttcaggcact gcttactgaa
     8461 actcctgcaa attccagtta ccgatatctg cgccagcgcc ttccccgttt tcctgaccat
     8521 ccgcccccag ggtgtaaaca tcaaacagtc ctttttcacc cggactgaga tactgataat
     8581 cattgcccca cggatctttt ggcagtcgtt taatgtatcc accggtacgg tagttacggg
     8641 aatccgccat attggccggt tgctggatca gcgcctcaag cccctgttct gtggtcggat
     8701 aacgcccgtt atccagtcgg tacatatcca gcgcattctc cagcgccacg atatcgctga
     8761 tggctttttg ccgatccgct ttctctttgt tgcccaacag gttaggcacc accagacttg
     8821 ccaggacgcc aagaataaca atcaccacca tcacttccag cagggtaaaa cctgcccgtg
     8881 gtttttgtgt gcgggataac gaattcatgt gataactccg taaattacat tccaaccata
     8941 ttattcagtt gcagcatcgg ctcgaggatg gcgatgacaa taaacagcac cacgcccgcc
     9001 atcatcacca ccagcgcagg ctcaaacagc cccaacgcca gccccacctg ggtatcaaat
     9061 tctcgttcct ggttaactgc ggcttgctca agcatggttt ccagttcgcc gctctgttcg
     9121 ccggaagcga tcatgtacag catcattggc gggaacagcc gcaactccgc cagcgcggcg
     9181 cgcagactgc ttccttcgcg gacgcgatct gccgccagca gcagttgttg ttcgacatag
     9241 cgatttgccg acacggcggc agcggtctga atgccttcca gtagcggaac actgctggcg
     9301 gtgaggatgc ttaacgtgcg ggaaaaacgc gccgtattca gcccgcgcgc aacacgcccc
     9361 gtcacgggca aacgcagcaa ggttttatcc cagcgcaggc gcatcgcagg atttttgagt
     9421 agccgttgcc ccagcaccag aagccccagc aaacccgcca gccagtacac gccgctggtc
     9481 tgtaacgtgt cgctcatggc gatgagcatt cgggtggagg cgggcagcgc gtgtccgaga
     9541 tgatcaaact gttcgataat tttcggcact actgccgtca gcaaaatagt gaccacgccc
     9601 gttgccacca ccagcagtac cagcgggtag agcatggcct gcagcaggcg tgatttcaga
     9661 cgctgccgct gttcggtgta atccgccagg cgattgagca ccacgtcgag atgtccggat
     9721 ttttctccgg cagcgaccat cgaacaaaac agggagtcaa agacgcgggg atgttcgcgc
     9781 aggctgtccg acagggtata accttcctga atccggctgc gcagcgccat tccgaggctt
     9841 tttacatgca gtttttcgct ttgctcactg accgcctgta agcaggtttc cagcggcatt
     9901 gctgcctgca ccagcgttgc cagttgacga gtgaacagcg caagatctgc cgccgccacg
     9961 cgacgatgtg cgtgccgccg acgctgcaac attccccctg ccgatgcatt catccgggct
    10021 tcaatgtgca cgggaatgag gtctttacca cgcaacaatt gacgggcatg acgcgcggaa
    10081 tccgcctcaa tcatgccttt ggttttgcga ccattacgct ccagcgcctg atagtaaaac
    10141 agtgccatta cgcctccatg gttacccgca gaacttcatc gagagaggtt tctccggcga
    10201 gcactttctc aatgccgttg ctgcggatac ccgcagagtg ttgccggaca taacgttcca
    10261 gctccagttc cccggcctga cggtggatca aatcacgcaa tgtggcgtcc accacaatca
    10321 gttcatggat agcggtgcgt ccgcgaaaac ctttgtgatt acaggcgggg cagccctgcg
    10381 gatggtacag cgtgacggta cgcgcatcgg taatccccag caggcgtttt tcttcgtcag
    10441 tggcaggcac ggactggcgg caatcgggac acagcgtgcg aaccagtcgc tgcgccatca
    10501 cgcccgtcag actggaagag agcaggaaag gctccacgcc catatcctgc aaacgcgtga
    10561 tcgcccccac cgctgtgttg gtatgcagcg tggaaagtac caggtgtccg gtcagtgaag
    10621 cctgaacagc gatttctgcg gtttcggtat cgcggatttc accgaccatc accacatccg
    10681 ggtcctgacg caaaatcgcg cgcaggccac gggcgaatgt catgccgacg cgggtgttaa
    10741 cctgcgtctg accgatccct tcaatcatgt attcgatagg gtcttcaacc gtgagaatgt
    10801 tacgcgagtg gttgttcagc tcctgcaatc cagcgtacag cgtggtgctt ttgccggaac
    10861 cggtcggccc cgtcaccaga aaaatgccgt gcggtttgtg taacagctgg cgcaactgcg
    10921 cagtcagttc aagacttaaa cccagacgct ccagcgtcag gcgagcctgg tttttgtcca
    10981 gcagtcgcag caccacccgt tccccccagg cggaaggcat ggtagataca cggacgtcaa
    11041 tcgcccgacc gcccagcagc agcgcaatac ggccatcctg cggtacgcgc ttttcggcga
    11101 tatccagccg cgccatcacc ttgatacgcg agaccagcag cgaggccagt ttacgaccgg
    11161 ggcgcaacat ttcatgtaat gtgccgtcaa cacgaaaacg gatcaccaga ctcttttcaa
    11221 acgtctcgat gtggatatcc gaagcgcctt ctttgattgc ctctgccagc atggcgttga
    11281 tcagtttgat gatcggcgca tcgtcgtcac tttccagcag atcttccgtt tccggcagtt
    11341 cttcagcgag ggtaaaaaag tcctcggcgg aaccgagatc ttccatcagc tggcgagctt
    11401 cggaagagtc ccgctgccag accgcattca gccgctgttc aaattcagcc tcgtcgattt
    11461 gccgcagtgt aaagggcgca ttcagccccc gctgtagctc ctgtaaaaca gagagcgaca
    11521 acggatgaac atggaggatc tccagcgacg cttcgcacca tgccaccagg ccaaaccgac
    11581 ggctgaaact gtagggcaga cgcacggtgt tagcggtggt ttcctgtgct acaggcacca
    11641 ttaacgcgtt ctcccggcat tgaggaacgc gcgtacttcc ggcggtaagg cctgattttg
    11701 cgctggcaat atcggctgcg cggtgtgcgg catcagggtt aagccttgct catcgcggta
    11761 gatttgctca gcgcgcatgt agttatattt gcgctgcgac acgccgtctg ccgccatacc
    11821 gtcacgcaaa atggtcgggc ggataaacac catcaggtta cgtttttctt ttttatccgc
    11881 cgtcgattta aacaggttac caatcaacgg gatatcaccc agcagcggca ctttcgccac
    11941 gctttctccc gcctggtcgt ccatcagacc gccaagcacg atcagctcac catcgttagc
    12001 cagcacggtg gttttcagtt tgcgctcgcc aaacacgaca tcgaggctgg tctgcccttc
    12061 cactttcgag acttcctgct caatcaccat ctgtaccgcg ttaccttcgt taatctgcgg
    12121 cgtgactttc agcatgatgc cgactttttt cctctctacc gtattgaaag gattgctgtt
    12181 attggaacca acggtagagc cggttaatac cggaacgtcc tggcccacca tgaagaaggc
    12241 ttcctggttg tccagcgtgg tgatactcgg cgtggagagc acgttcgagc tggagtcgtt
    12301 tttgaccgcc tgtaccagcg ccatccagtc gcctttcacc acgccaaccg ccgtaccact
    12361 aaagccagaa aggagctgag cgagcgtgga gagatcgccg ttagtatccg gatttatggt
    12421 ggtagcaccg ttttcgctga tcaccgtgga acctttctgc ggcttggctg cagaaatcgc
    12481 tgcccccagc gtaccaatag ggatttgcgt accgttggca aactgcatta atccagcatc
    12541 ttttgacccc cactgcacgc cgaaattgat attgctacct tcggcaactt ccacgattaa
    12601 cgcctcgaca tgcacctgag cacggcgaat atccagttgt tcaatcacgc tttgcagcga
    12661 ctgcataatg tcctgcggcg cagtaacaat cagggcatta ctgtgtttgc tggcagcgat
    12721 ggagacaacc tcacgcccgc taccgaccgt gccttccgcc tcttctttag ccgccgtgag
    12781 cgtaccgctg acctgcttca gcacatcgac cagatcttcc gctttgctgt atttgagata
    12841 gaacacctgg ctgttgccgc tgcgctccat ttctgaatcc agccgacgga tcaggcggcg
    12901 cattttgtct cgcgtggcgg ggtcaccact gacaatcacg ctgttggtgc gttcatcggc
    12961 gacaatttga gacttcagcg ttgctggctg gttctcgccg ctgtttttgg tcaggctttc
    13021 cagcacgcgg gcaatttccg aggctgaggc gttatccagc gggatcacct cttcggtgcg
    13081 attacccgcg tgatccacgc gctggatcac ttccgtcagg cgctccacaa cggaggcgcg
    13141 tccggtaagc ataatcacgt tggagggatc gtaattaaca acgttgcctg agcctgcgct
    13201 gtcaatcatc tggcgcagaa tcggtgccag ctcgcgtacc gacacattgc gtaccggcac
    13261 gactttggta accatttcat cgcccgcgta gttgtcgctg ccttcaccga ccagcggcag
    13321 cggctcgact ttcgcggcgc ttgatttcac caccttcagc acgtcgtttt ccatcggtac
    13381 gaccgcatac ccctgtgctt ccagcaggtt aaggaataac tggtaatact ggcgttcatt
    13441 gagcggagtc atagtgcgaa tactcacttt cccctgaacg cccggcccca taatgatggt
    13501 tttattaagg ttagcgccga cggtttcgat gaacgatttc aggtcagtat ctttaaaatt
    13561 agcggtgaaa gtggcttctt ccgcccagac cggtgaacta cacaatgccg ctgccagcat
    13621 cagcggcagt aaacgctttt ttgttttgag gccagttgtc ttcttacgcc agatagacaa
    13681 cgtcatatca cgccaaaaca cgatgaatga ttctcctgga ttaaatgcgg ttagcgcagt
    13741 gcgatggaaa tgtcgtagcg cgcacccttg cgtaaaaccg taagttgaat ggaatccatt
    13801 gaaggtaact gccgcatcag agcaatcatt gctcgtggat cagtgaaatc ctgctgattt
    13861 agtgcaatgg cgatatcgcc ttccctgaaa ccgctggcat cgaacagaga acgatctgcc
    13921 cccggcttca ctgcataacc gacaattccc tccttacgca caggcgtaag ctggatatag
    13981 ttaaaaattt tctgcggatc tttcgccagt gcctgacgca cggcagccgg gatctcaact
    14041 ggcgcactgg cagcaggctc agcaacagct tgctttgctt cgtcgctgac ggcttttttg
    14101 ctggtgaccg ccacaggggg gcgttcctcc tctgccagac tcagacgttc cattttcccc
    14161 tgatagcgca gcatcacatg gtcgcggttg atttcctcaa tcactgcgtt gtgagagcca
    14221 agccgttcac cctgcaaata gacctgctgt ttgccgcctt cttcaatcac cgcgccgggt
    14281 ctggcaccaa aggcgatccc acgcagcacc acattaagac gcgtttctgc cacaggcgca
    14341 gattcaggtt gttttaccgg tgcggcgacg ggttgatatt tgccaaacca gttttgttgg
    14401 ctgactaatt gcacgtcatt tttatcgaac ggttttgcat ccgcacggag cggtttattc
    14461 gccgatgagg aaaccaccgt atattccgca gaaaaggaga aatagcgcca gagtgaatac
    14521 gccatttttg cagaaataat aagcagcatg agccagaaca gcccgcgcac catttttcgc
    14581 agatgctctt tgtctgtatt aagagattgt ctctggctaa gaatgtgacg aattttagtc
    14641 agccattgaa tgagataaat tcgtgcgtca cgaaaaacaa cccgcgccaa tttatatcta
    14701 cccgacgtta tgctttgact attccacagg tggtacgatc cagtttccgc tgatactggt
    14761 tacctgtacg tgtatcatta atcgttatgc tgtaattaat tccctcggtg atcattaatt
    14821 tcaccgttgg gtcagcgcac atttgtcgtt gatagctggt taaaaaggcg tcaggtgtct
    14881 gcgtggtctg agtacccgat tcgctgataa tggtcatttt taccgtcgtg ccactacttt
    14941 gcgccagcac taaggtatat cccgcagatt taatcggcag gttttggctg atattttgcg
    15001 cctgtttttt cgccagtaaa ctggcgtttt cattatggct ggcgcagcca cttaataatc
    15061 ctgtaacgct caacaatagc gatattaata ccctccctgg catttgtttt atcgacatgg
    15121 taaataatct ctaaggttat taataagagt taaaatgtca ctttgataat gacgttgtta
    15181 tcattaaaac aatgcctgta gataaagtgt tgctataccg cccagactta aacacggtcc
    15241 aaaaggcagt gtggttgatc ctctttttgt aataacggca tatatcaggc cgcagcatga
    15301 ggcgattaaa gcaacgttgg gcagtgataa cgcccccacc cagccaccta aagcggcgaa
    15361 gagtaataca tcgcccatgc ctaatgcttc tttacgcaga actattccgg ctatccagcg
    15421 cagggagtaa aaagtgataa atcccaccag tacgccggtg actgcgtctt gtagcgtgag
    15481 cggactctgc tgcgcccatg ccgcaattag tcccgtccac aatacgccct gagtaaaaac
    15541 atcgggcagc cattggttat cgaggtcaat gacgctcgcg gcaatcagcc aggcggataa
    15601 tatcatcacc gccagcgccc atccactttc cggccacacc aggctcgcca gcaaaaaagc
    15661 gagtgccgtc aataactcca ccagcggata acgcttgctg attttcgcct gacagtcgcg
    15721 gcagcgccct ttgagcatca accaggagag cagcggaata ttgtcacgaa cgcggatagt
    15781 ctgctggcaa tgcggacagt gcgagcgtgg cagcgccagg cttatttttg actgcgtact
    15841 cggcgtttca ccatgaaact ccgccatttg ttggcgcagc atgatggggt aacgccaaat
    15901 caccacattc aaaaaactgc cgatgatcaa tcctccgacg gttgccagta tgggcatcgc
    15961 cgcggggtat tgctgaaaaa catcaaaaag catggttaaa ggttgtttgt tgtaacttgc
    16021 cggatgcggg cctgcgggtg tatgccatat ggctttcctc caggcccgat aagctggcgc
    16081 atcgggcata tgtgttgacc cgatgcgcct tatatcatgc cggatgcggc gtgaacgcct
    16141 tatccggcct acaggcttac tcggcagaca tcttatgctc ggtaaccttg ttaatggttt
    16201 ccggcccttt ttccggtttc ggcagcttga gtgacgccag cgtgctgtaa gccgaagagc
    16261 tcacaccgcc ctggaaactc atctccgttg ctcccggcaa ctggtaagca cttgcacccg
    16321 gattccattt cttaaagaac tccgaaagat ccgtctgggc gacccaggag gcacacagca
    16381 tcagcgtgtc ggcagcgtta ccattggatt cagcacagta attcttgcca ccaaaggtgc
    16441 tgttaccaac atcatcgccg cgtgctttac gatgcatcaa ctggaacagg ttccagcctt
    16501 tcatcccttc acgctcgctg tagaacgctg gcagcttgcc atctggatac cacttcgtga
    16561 tatcaaagtt ttcctctgcc cactccttca actgtgcgta catcagcaga cggtcacccg
    16621 caccgccgcg cgcccatgcc tgaccgttgc tctcctccag atattccggc gcgacagtaa
    16681 tatcgtcagc gacacggttc atcttgccga gatagcgatc ctgcatgtac agcgccagca
    16741 cgttgttcgc tacttcagtt gcgccaggaa cagtcagcgg cgtttcggcg gcgttgtgac
    16801 caacttcgtg ccagatcagc cagtcgttca gcggcgtcgt cggcagcgtg gtgctgttcg
    16861 tcgagaagct gctgttcatt accggataac ccgaatgcgc atcaccgatg gagatctgca
    16921 catcgttggt gaaacgatgc ttgtggcccg tcaagttttt ataggtaaac atccggtgct
    16981 taccgtcttc atcattacga ccgtagaaat cattcatcga gctggcaaag gtatccagat
    17041 ctttagcgaa ttctgctaca ccaccagtga aattgctggc ctcaaggttc ttcttcggcg
    17101 tggtatagac gaacgacgca gactccagct cgcccagcgg cgccggtgag tccagatcgt
    17161 ttttccattc gccgtcttta tagaacggcg cttttactac accggtaaag gtgaagttag
    17221 cagaaacatc atccttactg tcgcccttga tataaatcag accaccataa ggcaccttga
    17281 aggtcacttc accgttagcc tccagagtat acgttttagt cactcttggc ggacggttca
    17341 gcgcaacttc atgcttctca cgtccagtca ggtcgtcagc cagcgccacg gtaacagtca
    17401 ctgggactga cgccgaagac ttaatggtga cgtcctgctg ggccggtgcc cacaggccgg
    17461 ttgactgcat gttacccgca aaccatttgg tcggattcga gtacaggctg atgttttccg
    17521 taacgctctc accctttgcc gatacggatc ctgggtactt ctccacatca accttaatgt
    17581 tcagatccca ccaggaacgg cccagcatca gacgcgtcag cggtttttcc atatagttga
    17641 gcggatagct tgggttcatc atgcccgctt tgctgctacc gtcaccgtag atcatgttgt
    17701 tatcgaccag cgattttttc agatctgcag agcacttggt gccgcctgca taggcatcat
    17761 tggcgtagca gttcaggaac tcggtgaacg ttttaaagcc cagctcatct tccttgccct
    17821 cttcgtaacg atatttcgta tcgttccaca gccagaccga catgttctgg tacagacgtt
    17881 ccagatcaac actgttcaga cgctcacctt tactgccttt ggtacggaaa taaagctcat
    17941 gctgatacag gcgctgaatg ttggtgccta aatccgccgc ctgcaccatc gctttcgcgg
    18001 tgtcggcgtc aagattcagt tgcgtatagc gcggaacata catgccacct gttaccggaa
    18061 catccgtgcc tgggcggcgc tccaaacagt taatctcgta atggtaagtc gagtccttac
    18121 actcctgtaa cccaggaaac ttctcaaaga tttttgcctt tgccgcttcc agagattctt
    18181 ctgttgtgta ttccgcttca tcaataaagg cataacgcgt tacctgtttg ccctcaactt
    18241 cctcctgcca gctcgcaact tccagtttcg gcttgtcatc aggcttgttg tcttgctggt
    18301 atttccaggt cacttcccct gtatttgggt cgatggtgta cggcggttgc gcgccgtctg
    18361 cagcaggata acgttcataa acccaaatgc cagtcgcgcg acgctgacga acgcgatccg
    18421 gatacccttg cggatcgttg ttcaccaccg atttgttcag agccattgac agacccgcgg
    18481 catccagcag acgcacaaaa ctggacgcgc tctcttcctt aagattgctc atcacgtttt
    18541 ccatgatcag caccgagcca cctttgttca gataagcgat cagatcggtc acatcctgct
    18601 gagtcagctt cggtttgctg gtatctgcac gcagaggcac agcataggga tcgccagacc
    18661 actgagtcac atattcaaag ccgttgagga tcagcaacgg aatctcttcc ggattcagat
    18721 cgccatagct ggtcaactgt ttaaccgtga taccagtgaa atcctcatgg aaagcaaatg
    18781 gtgcactatt tcccaatacc tggcccgctt ttttgaaata aacgttctcc aggttggtgc
    18841 cgacagtcat gatgctcttg gtatttggct gccagatgtc atttgacaag tagcgcagga
    18901 cgttctgcat aaagtgcttc atgtcatcag aatcaccgct gagcgtacac tcacctttac
    18961 tattaacacc accgccccaa ctgtaaccgt tcgggcaacg caggatgctg ttgtagtgcg
    19021 ggttaccgat aaccatcagt ttgccttcac cgacttgccc cagcgaaata aacggcaggt
    19081 tgaaagtcgc agtatcgcgc gtaacgttct ctggctgcac aatggaaggc gcttccgtaa
    19141 tgtacgccag ctcattttta tcccaggcgc gtttttcgcc aaacgccagc cagtagtttt
    19201 tatcattacg cgccatcaga atcgggaatg ccgagttgga gatatttacc accgcctgac
    19261 cgcgcgcgtt accggtgctg ccatagaagt tggtagagtc atggaagacg tggaacttgc
    19321 tgacagattt gtaacttgta tccacacccc acagcttgtt gataacgccc tgaatctggc
    19381 cgtcattaac attgcgcgtc gtcagcgaga accagcgagc ctcgttacaa ccgtcggttt
    19441 tcgcacaaat cgcggtatcg atctctttgg cttgacccgt cttaaactgc tcaataaatt
    19501 cgtttggcag attaacaact tgctcacctt catccagcgt cgcaccgttg gataacgaga
    19561 gattgataat ctcgttgatc acgttgggat attcggcaaa gaccttgcgt acatcgtccg
    19621 gcacaacacg ggtattattt tgcccggtcg tcgaatagcg atgaataagc tgatcaatat
    19681 tcgcgccgcg aacttcgtcg cccagctcag tcaacgcaat ggtcgactta ttgccgcgta
    19741 ctgagcccag ttcaaaggtg tcgataccaa aggagatggt ttcgccccag ctgaagttga
    19801 atttgccgtt ttcgccagtt acgccacggc ctgagctggt gtagtaattt acgccaacca
    19861 caccattgcc catgctatct accagtcggc cttcagagag aatgatttcc gtaggttgat
    19921 actgataaaa ctgttccgcg ttagccgaca cgaaggaagc gttcagatcc ggttttgttc
    19981 ccggagtggt gacgggcacg accggtgaag tatgagtgga tggcgcttta tcggtcgcgg
    20041 cattgttttc cacctcttca ttgaccagct ttttgaactc ttccggtgcc agatcgattt
    20101 gcttatacag cgagtcgaag cgtttactct cgatcactga ggagaactcc aggcacacct
    20161 gttctgtatc cgccgggcaa ctgttcatgg aggtgactag cgagagcgca ttgcttttct
    20221 tgtcatcgga agccgccagt tcttgcgcgt cctcaagact aaacgacact ttttcaaccg
    20281 cacgcaggct acgcgcagct tctgactgag tgttgaaggt ggcaattgtc gtgttacccg
    20341 ccacgcaagt aacgtcctca ccaggtgtaa atgtaaagcc atcgctggat tcaccattac
    20401 aggtagcacc agttacccgc tggcttccgc ccagggtcag ataacccgtt ttcgtaggaa
    20461 caggttctgg ctccggttct ggctctggat caggtgtcgg ttccggcgtt ggttctgggt
    20521 ccggcgttgg ctcaggcgtc ggctccgggt ttggtgttgg atcaggtttc acttccggca
    20581 aagaccctgt tccagaatct acaggcggcg tatcggagga agatccggag ccaccgccat
    20641 cacaaccggc ta
//
LOCUS       DAPHCV010000091        20163 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.091, whole genome shotgun sequence.
ACCESSION   DAPHCV010000091 DAPHCV010000000
VERSION     DAPHCV010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20163
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.091"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..271)
                     /locus_tag="RH033_003369"
     CDS             complement(<1..271)
                     /locus_tag="RH033_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992509.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVL"
     gene            complement(332..979)
                     /locus_tag="RH033_003370"
     CDS             complement(332..979)
                     /locus_tag="RH033_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDV2992510.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGAEAIRAL
                     ATQLPVFRQKLSDGWYQIRIAGRDTGENELSARLNEPLEHGAVIHIVPRLAGAKSGGI
                     FQAVLGAALIAVAWWNPVGWLGAAVVSGMYAAGASMILGGVAQMLAPKPRTPRTQTTD
                     NGKQNTYFSSLDNMVAQGNPLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(877..1620)
                     /locus_tag="RH033_003371"
     CDS             complement(877..1620)
                     /locus_tag="RH033_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV2992511.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     DYFRMAPEDWLQAKMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMSDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHLAWHASAFTGICNDLAAASTFG"
     gene            complement(1626..2324)
                     /locus_tag="RH033_003372"
     CDS             complement(1626..2324)
                     /locus_tag="RH033_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDV2992512.1"
                     /translation="MQDIRQETLNECIRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRKYQAYPIQGSGFEMNGKGSSARPTLKVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSEADPEQEVISRWRIEQCSELNAASAAFVLSTPTETDG
                     AVFPGRIMLANTCTWIYRGDECGYNGPAVADEYDQPTSDISKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            complement(2324..2662)
                     /locus_tag="RH033_003373"
     CDS             complement(2324..2662)
                     /locus_tag="RH033_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV2992513.1"
                     /translation="MANTEEFYWLPEDGMKTENKPSIKTVRFGDGYEQRSPNGLNHSL
                     RVFTCDFRVEANERNSFELFLARHGGYKSFFWRPPGINRKIRVVCRTWSATEHITYTD
                     FSCQFDEVVI"
     gene            complement(2655..5888)
                     /locus_tag="RH033_003374"
     CDS             complement(2655..5888)
                     /locus_tag="RH033_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDV2992514.1"
                     /translation="MAEQTSRLAIIIDSTGAKNNADNLASSLVKMTQAGETAANSAGK
                     VTKATEDEKNALAKLKAAIDPVGAAIDTVGRRYSELKKFFDKGLIDKEEYEFLVRKLN
                     ETTEELSGVAQAQREAEKAGKLAAAQQEAQAQAFQRMLDKIDPLAAALRNLEQQQDEL
                     NAAFASGKINGSQFENYSRKIQETRRELTGEAQAEREAAKAHDEQVAALQRLIAQLDP
                     VGTAFNRLVEQQKQLNEAKAKGMLSPEMYEELSGKLRAMRSELEVTRSQLSKTGMSAK
                     QTAFAMRMLPAQMTDIVVGLSTGQSPFMVLMQQGGQLKDMFGGIGPAIKGVGSYVLGL
                     INPFTLAAAAVGVLGLAYYKGSQEQDEFNKSLILTGNQLGTTSGQLADIAQRAGNAAD
                     STTGAAAAVLNQLVRSGKVASSSLEQVTTAIVKTSEVTGISTEQLVNDFNEIAKDPVS
                     AISKLNDQYHFLTLATYNQIKALQDEGNQQEVARIATEAYSSSMIQRTNQIKENLGYL
                     ETAWKAVADSAKWAWDSMLDIGREASLDQKISDVLRQIDEIEKNTRPGVFGLGGIGDG
                     GAQNKKLARLKQQLGVLQAEKIAQDVLNSSINDYNKRQQEGIELRQRADAFSKQYQTR
                     EQQRASELAKLEKLKNQYSKEEYSNLIAQINERYKDPKQPKAKGYSDDAAQRMIDHLN
                     QQNALLSSQAELTVKLSSSEQELVKWRQQIADLESRPSSKLTQDQKSLLLHREEITAL
                     MEKNVAIEKNNRLIKESAEIAAWRDSLQASIDNRQQGYDIQIAGYGVGDKNQQRQQEL
                     LRIEREYNNQRLQLERDYADKSRGMSNHVFQEKMQALNDALEREKEIVSQINEQLDIQ
                     AGDWISGASQGFNNWLDDTKDIGAQIKSTTTQMFDGMTDALGDFVTTGKANFRSFATS
                     VISDLSRIALKASITGIFDSISNSSSGGILGTIGSAISKFIPNAKGGVYESPSLSTYS
                     NGIYDSPQFFAFAKGAGVFGEAGPEAIMPLTRTSDGSLGVRAINSKSGNGGRDITYAP
                     VYQITIQNDGQNGEIGPQAIKALMGMVDQRVQGALLNMRRDGGMLSG"
     gene            complement(6360..6719)
                     /locus_tag="RH033_003375"
     CDS             complement(6360..6719)
                     /locus_tag="RH033_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992515.1"
                     /translation="MVASSDNDQYRSRNALIRRHIEKMDASLHVGTKEFDIAKVSEVD
                     SVDDLLIDNAARYLLKDWKGVGELVDGVEVALEYTPERGIALLKQNPELYWQILAEAV
                     SIAQGKEQQKQDTIKKP"
     gene            complement(6870..7832)
                     /locus_tag="RH033_003376"
     CDS             complement(6870..7832)
                     /locus_tag="RH033_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992516.1"
                     /translation="MSSGAKVISAFIRETVAGTTPASGDWSLLKRTSWGVKPTQNKGE
                     NNEIGGSRMAQGATPGTVDVGGDVGTKFRWGQHDDFLASCFGAEWSGDSLTMGNERIT
                     FSLATYASDVGIASVVRGAQVGSWKMQIPNDGDITATVTFAGLDWESKADDTNFIKGE
                     PVDSAGKLRYSFKEVSAVSLNGVAGGNGFCIDSFDIQFDNKLQTQRCIGTGSPYAGAN
                     IPTTFTPSGTVTLSWSKAAWEIWSKTLTGETVPFSFTLSNGEGAYTFSFPKVQVSGEW
                     PDGGNTDIIQVQLSITAADEAPTITRKKASPAAVIAKASAEAIS"
     gene            complement(7859..8251)
                     /locus_tag="RH033_003377"
     CDS             complement(7859..8251)
                     /locus_tag="RH033_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HDV2992517.1"
                     /translation="MTFTEIRNTVISRMTAQTIIDGKDVLYPNAPTFDPSGKLIWARL
                     SNIPGQAGVNEIGAGPIVYRTGIIIIQLFVPAGSGSKLITETADKLRELFEFQDDDRL
                     SYQAVSSIAVGEKNGWFQLNLQIPYRAL"
     gene            complement(8248..8628)
                     /locus_tag="RH033_003378"
     CDS             complement(8248..8628)
                     /locus_tag="RH033_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDV2992518.1"
                     /translation="MAKAWDIEPSIFAGMIEEDVGLKIRYIAIQILTAIDIAAPVDTG
                     RFRNNNLVSLQHPDFGISDNVDPNGTIAVQRGIGVISKAANYGVIYIQNNLPYAEALE
                     NGHSQQAPTGVYANAFHGVLQAYK"
     gene            complement(8629..9012)
                     /locus_tag="RH033_003379"
     CDS             complement(8629..9012)
                     /locus_tag="RH033_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDV2992519.1"
                     /translation="MGLREEIQSEVAAAFDEDLADAVSDFSGSYVTHRHWDPVTETGG
                     ESTAIYTGRGVLTRYKLGRIDGINILHGDLKLTALVCEVTDKPAVDHIIEIYDPVSRQ
                     LQRYEVITASVDPSASVYSIQLRRA"
     gene            complement(9012..9407)
                     /locus_tag="RH033_003380"
     CDS             complement(9012..9407)
                     /locus_tag="RH033_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein singed"
                     /protein_id="HDV2992520.1"
                     /translation="MITYVTCDDVDNAFGNAWTSENAKNKAVLMANAWLNGFNLKINP
                     SRIPEEVKLAGAYAARIASFGKLFQQKNDSGVVISKAVSADGVSVSKSFSELPANSTA
                     LLEPDLQLAIALLKPYGLSRSQVRVVRGG"
     gene            complement(9411..9587)
                     /locus_tag="RH033_003381"
     CDS             complement(9411..9587)
                     /locus_tag="RH033_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004121546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992521.1"
                     /translation="MAGLTKEQRAQREAAQKTQIELVVMVTDYQMFPGAPTIANVHPD
                     EVDNWKAMGWKTQE"
     gene            complement(9630..10769)
                     /locus_tag="RH033_003382"
     CDS             complement(9630..10769)
                     /locus_tag="RH033_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 coat - protein 5 family protein"
                     /protein_id="HDV2992522.1"
                     /translation="MAGNTLTGLIPTIYTALDVVSREQVGFIPAVAKNAKADAAAKDQ
                     TVTAPVAPEAKTEDIVPGPSAPNTGDQNIGTVDVKITKSKMAPVKWNGEEQLALGPSG
                     TYNTILADQFKQAFRALANEVEADLGALYFGASRAVGTAGTTPFGVKDDLSDAALARQ
                     VLEDNGAPTTDLQMVLGSTAIANLRGKQSVLFKVNESGTEQLLREGVLGRLEGFNIHS
                     SAGVKRAPKVAATGYLVNGEKKEGDVLISIDTGSGSISAGQIVTFAGDPNQYVVAAAT
                     SNLITLAAPGLRQDLADDTAITVVGAFTANMAFDRNAFLLASRTPAMPEGGDNADDVM
                     NVTDPISGITFQIALYRQYRQVRYEVGLAWGVSSVKPAHGCLILG"
     gene            complement(10868..11632)
                     /locus_tag="RH033_003383"
     CDS             complement(10868..11632)
                     /locus_tag="RH033_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992523.1"
                     /translation="MKLKTVEINGKQYAEIDTAGLPVYVHDDGKEIGFDAPLATKKIT
                     ELNGEAKNHRLAKEAAEEKLAKFAAIEDPKKAIEALEMLSKIDQKKLLDAGQVDQVKA
                     EITKNFQQQLDEEKQRSQMLEKQLYDSMIGGSFAGSKYIADKIAIPADLLQARFGQAF
                     KVEEGRIVAYDASGNKIYSRAKPGELAQFDEALEFLVENYPQKDYILKASGNNGGGSR
                     PTQHDIGQKTMKRSAFDALDVAGKQNALKDGITIVD"
     gene            complement(11737..12849)
                     /locus_tag="RH033_003384"
     CDS             complement(11737..12849)
                     /locus_tag="RH033_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001645719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992524.1"
                     /translation="MQTVNERLRDESIAHAVWISRYSTGVAARMVKILNDSDAELTAR
                     LLIALDSLDPGNFTVKRLESLLASVRKVNRTAINSMFTSLSGELNELAIYEAGYQLSL
                     FDSMLPDFVADVHPLVGISPDALYAAAMARPFQGRLLSEWASDLEADRLRRITNTVRQ
                     GFLLGDTNEQIARKIRGHVSKGFQDGALQMSRANAASIAKTAVGHLAATARESFASAN
                     NDLIKGKQWLSTLDNRTTPQCRIRDRLKYTLNNKPVGHSVPYLQGPGKIHFCCRSTET
                     FILKSAKELGIDVRDISPAERASMDGVVAGDTTYREWFLRQPYTRQKQIVGETRAKLI
                     RDGGMSPDEFYTDKGEWLTLKQLRERDAQVFRKAGI"
     gene            complement(12833..14239)
                     /locus_tag="RH033_003385"
     CDS             complement(12833..14239)
                     /locus_tag="RH033_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4055 domain-containing protein"
                     /protein_id="HDV2992525.1"
                     /translation="MADISTPNLDYNDMLEAWDINDALMGGTLEMRRQGENYLPKWPN
                     EDEDAYKKRLSVATLLPVYEESIKQNIGRIFAEPTVLSEETPAKIREYAENIDMEGSR
                     LDVWAQQFFSLAFQYGVAHALVDYPRTDMKEIRTKADENATGGRPYVTMLNPRQVIGW
                     KSKVEKGKVVLTDLRIKEVIIIDGDDFGQKKVEQIRHIMPRRVEIYRRSEGTNGESVW
                     TLHESWNTSRDDIPLVTLYTKKTGFMRGTPPLLNLGLLNIKHWQSQSEQDNILHVTRV
                     PLLVAYGLDRNEELTVGASTATIFEDRTKNGLEYVEHSGAAIESGETSLEKLENQMRH
                     AGAKLLRAENTSTKSVDQTNEERMQENSPLYTMANSLEDALDNILQIMAEWIGESCGG
                     NVDVRTELDVSAQVFDSSSALAVQSLRQGGDIRQIDAVRVLQALKFIDQDSRPEEVID
                     ELKNQSVMLMEINDANRE"
     gene            complement(14242..15543)
                     /locus_tag="RH033_003386"
     CDS             complement(14242..15543)
                     /locus_tag="RH033_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HDV2992526.1"
                     /translation="MISPTLNVPQARFLSMPHKFKAYIAGFGSGKTWVGCGGICKGIW
                     EHPGINQGYFAPTYPQIRDIFYPTVEEVAADWGLNVKINEGNKEVHFYYGRQYRGTTI
                     CRSMEKPQTIVGFKIGNALVDELDILPKEKARTAWRKIIARMRYKIDGLRNGIDVTTT
                     PEGFKFVYEQFVKAVREKTELASLYGLVQASTFDNEKNLPADYIPSLLESYPPELIKA
                     YLRGQFTNLTSGTVYHQFDRKLNNCEEVEQPGEPIYIGMDFNVGKMAGIVHVLRLGLP
                     CAVTEIINAYDTPDMIRIIKERFWLYDGNDYRKVREIYIYPDASGDSRKSSNASTTDI
                     AQLKQAGFNVVVNSSNPPVKDRVNSMNAMFCNANGERRYKVNVKRCPLYAESLEQQVW
                     DEKGEPDKKSGNDHPNDAGGYFIVKQFPIVKPTGRVTSLRI"
     gene            complement(15524..16618)
                     /locus_tag="RH033_003387"
     CDS             complement(15524..16618)
                     /locus_tag="RH033_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDV2992527.1"
                     /translation="MSRPDWGALQQEYIAEYTRSGISPVAWCEARGLNYATARRYIKK
                     TPKNAQTEMCKTAQKSAQKKSAQTAQKRNEKSQEKKPVFDAGLNEGDAEEFSFCPDEF
                     GISDQQAKFAMLVAQGKKPTEAYRLAGYEGQGATANSNASRMLRNARVFRAINYFRNQ
                     YQKRYTADLDLLVSQLMAIVLADPNQLAQFRRVNCRYCWGENHLYQWRDIAEFDKAAA
                     QASRDGKPEPEYGGLGFVDNAIPNPDCPKCCGEGTGQLYMADTTLLDGDARQLYAGAK
                     FGKFGVEILLEDKAAARRELLRLFSAGGALCADKRLRELEIERRRMENQKLRKEIETV
                     EDNEHPQPVAININVVDARVRSDEDDLSDA"
     gene            complement(16622..16873)
                     /locus_tag="RH033_003388"
     CDS             complement(16622..16873)
                     /locus_tag="RH033_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992528.1"
                     /translation="MIPYSKVESLAACRMTAQQIADVLDVDLNRLKENREAMTDFYAA
                     IRKGRAKGEAELRAALFKLARKGDAFALRELLRVDKNQD"
     gene            complement(16809..17741)
                     /locus_tag="RH033_003389"
     CDS             complement(16809..17741)
                     /locus_tag="RH033_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992529.1"
                     /translation="MRDDFCAFILTHGRPDKVLTYRTLRRAGYTGKIFIVVDDEDKTR
                     NQYMAEFGEQVLVFSKADIASRFDEADNFCDRRSIFYARNACFDLAKLVGCKYFIQLD
                     DDYHEFQFRVDRNYDQAYFPIRKLDAILSEMLAYYESIPALSIAMSQGGDFLGDNGGH
                     ASWVKRKAMNSFICSVDRPFSFMGRINEDVNTYTNLGRCGELFMTIGAVQLGQKQTQK
                     NSGGMTELYLDSGTYVKSFYSVMYAPSCVKISLMGASHKRIHHQVTWNNAAVKILHEK
                     YRKKTPCISMGVTNDSVFESRVSGSVPDDCTTNR"
     gene            complement(17734..18525)
                     /locus_tag="RH033_003390"
     CDS             complement(17734..18525)
                     /locus_tag="RH033_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDV2992530.1"
                     /translation="MSEKLKIVYRPLQELSPYAHNARTHSPEQVAQLVESIKQFGWTN
                     PVLIDEKGEIIAGHGRVMAAEMLKMDSVPVIVLSGLTDEQKKAYRLADNRLPMNAGWD
                     EDLLRMELSDLINADFDVSLTGFIPTEIDELLTDVLPGTGNEEEPYTTKIDTPVYEPS
                     GGKPDISELYDDTKTQELISRIRSASLEPDIEKFLLCAAERHTVFNFSRIADYYAHAP
                     SEIQCFFEESALVIIDYQQAIENGFVRMTQRMVEIMHGGEEEEYA"
     gene            complement(18663..20120)
                     /gene="trkG"
                     /locus_tag="RH033_003391"
     CDS             complement(18663..20120)
                     /gene="trkG"
                     /locus_tag="RH033_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkG"
                     /protein_id="HDV2992531.1"
                     /translation="MNTSHVRVVTHMCGFLVWLYSLSMLPPMVVALFYKEKSLFVFFI
                     TFVIFFCIGGGAWYTTKKSGIQLRTRDGFIIIVMFWILFSVISAFPLWIDSELNLTFI
                     DALFEGVSGITTTGATVIDDVSSLPRAYLYYRSQLNFIGGLGVIVLAVAVLPLLGIGG
                     AKLYQSEMPGPFKDDKLTPRLADTSRTLWITYSLLGIACIVCYRLAGMPLFDAICHGI
                     STVSLGGFSTHSESIGYFNNYLVELVAGSFSLLSAFNFTLWYIVISRKTIKPLIRDIE
                     LRFFLLIALGVIIVTSFQVWHIGMYDLHGSFIHSFFLASSMLTDNGLATQDYASWPTH
                     TIVFLLLSSFFGGCIGSTCGGIKSLRFLILFKQSKHEINQLSHPRALLSVNVGGKIVT
                     DRVMRSVWSFFFLYTLFTVFFILVLNGMGYDFLTSFATVAACINNMGLGFGATASSFG
                     VLNDIAKCLMCIAMILGRLEIYPVIILFSGFFWRS"
BASE COUNT         5145 a         5458 c         4163 g         5397 t
ORIGIN      
        1 cgagcaccgt ctcggagccg gaggattcaa atccctccgg cggtgtctgc tcctgctcac
       61 cggcccggaa caccaccgtg acgccggata tattggtatt cccctcactg tccagcaccg
      121 gcgtactgtt cagcagcacg ctttttaatc catccaccgg accttcaacc ggcccttcgc
      181 tgatggcatc gatcacactc agcagctgcg tggacttcag gttgtccttc gcttcgcgcg
      241 gggtatgccc cttactgctg cctttaccca ttcgtcatgc tccataaacg ataaaaccgc
      301 ccgcaggcgg tttcacataa aacgtttttc atcagcgacc aatcaccaca acctgaccac
      361 cgtccccttc gtctgccgtg ctgatttcct gagataccac acgtgacccc acgcgcattt
      421 caccgtacag caccggtagc ggattaccct gggcaaccat gttatccagt gaggagaaat
      481 acgtgttctg ctttccatta tccgttgtct gtgtacgggg ggttctgggt ttgggggcca
      541 gcatctgcgc cacaccgccc aggatcatac tggcccctgc cgcatacata cccgatacaa
      601 ccgcggcccc cagccagccc acagggttcc accatgcaac cgcaatcagc gccgccccca
      661 gcactgcctg aaaaataccg ccacttttag cacccgccag acgcggtacg atatggatca
      721 cggcaccatg ttccagcggc tcattcagac gggctgataa ttcgttttca cctgtatcac
      781 gcccggcaat ccgtatctga taccagccgt cgctcagttt ctgacgaaac accgggagct
      841 gtgtggccag tgcccggatg gcttcggctc ccgttttcac ccgaaggtcg atgcggcggc
      901 caaatcgttg caaatccccg taaaggcaga tgcgtgccat gccaggtgac gccagaggga
      961 gtgtgtgcgt cgctgccatt tgtcggtgta cctctctcgt ttgctcagtt gttcaggaat
     1021 atggtgcagc agctcgccgt caccacagta aatggcggca tgattcggca ccgatgaacc
     1081 aaagcagcac agcagcacat cgcccggctg cgccgctgac aacggcacct gatacagccc
     1141 tgtggcctcc agattatcca gatagagatt ctgaccgtga cgccaccagt catccccgcg
     1201 atgaaaatcc gacatctcaa ttcccgccag atggtaagcg tcccggaaca gcgtgtaaca
     1261 gtccgtcacc ccgtgctcaa agcgccgccc ggtgagatgc ggcacacagc ggaacttgtg
     1321 aatcgccccg cggcagacca gccaccacgg caaatcactc tgcacctgca gccgcctgtc
     1381 ggcttcactc agccagggca gaccaccggg atgactgtgg accagcgcca caatctcacc
     1441 ctgcattttg gcctgcagcc agtcctccgg agccatccgg aaataatcct ccggctcacc
     1501 ggagatattc acgcagggaa aatatctttc cccctccggc gttctcacca cgaagccgca
     1561 cgactccgct ggcgcacatc gtcgggcgtg cgccagaatc gctgattctg tctctgtcat
     1621 gggatttact gcgaaagttt gttaatggaa aggaagccgc caaagttgcc gacgttattg
     1681 cggaacttac agccgctcag gcatttactg catttatcct tcgagatatc agatgtcggc
     1741 tggtcatatt catcagcgac agctgggcca ttataaccgc actcatcacc gcgatagatc
     1801 caggtgcagg tgttagccag catgatgcgc cccggaaaaa cagcaccatc cgtttccgtc
     1861 ggagtagaca gcacaaaggc cgcgctagcc gcgttcagtt cgctgcactg ctcgatgcgc
     1921 cagcggctga tcacctcctg ctccggatcg gcttcgctgt ttccgttgac gaagttcacc
     1981 gcatccagaa aacgggcgta caccttacgc cggaccaccg ttccgccgac cagactctgc
     2041 agatcttccg ccatcccggt gaccatgccg tgcaggtttg ataccttaag cgtgggacgt
     2101 gcgctgctgc ccttaccgtt catctcaaag ccgcttccct gaatggggta cgcctgatac
     2161 tttcgcccct gccaggtaac cggttcacct ttttcgttct gctcattgca gaaaaagtaa
     2221 cgctccccac cgacctctgt cagatcaatt tcccagagca ccacgctggc cgactgctcc
     2281 gcacggatac attcattcag tgtttcctgt cgtatgtcct gcatcagatc accacttcgt
     2341 caaactgaca cgaaaaatcg gtataggtga tatgttctgt cgctgaccac gttcgacaca
     2401 ccactctgat ttttctgtta atacccggcg ggcgccaaaa aaaagattta tagcctccat
     2461 gccgagccag aaacagttca aatgaattac gttcattcgc ctcaaccctg aaatcacagg
     2521 taaaaacacg cagagaatga ttaagtccgt ttgggcttcg ttgttcataa ccatcgccaa
     2581 atcttacagt ttttatcgat ggcttatttt ctgttttcat tccatcctct ggtaaccagt
     2641 aaaattcttc cgtattagcc acttaacatt cctccatcac gtcgcatatt taacagagcg
     2701 ccctgcaccc gctgatcaac catccccata agtgctttta ttgcctgagg accaatctct
     2761 ccattctggc cgtcattttg aatagtgatt tggtatacag gggcataagt aatatctctg
     2821 cctccattac cacttttact attaatagct ctgacaccaa gagaaccatc ggaagttcgc
     2881 gttaatggca taatagcttc cggtccagcc tcaccaaaaa caccagctcc ttttgcaaaa
     2941 gcaaaaaatt gcggggagtc ataaataccg ttcgaatatg tactcaatga cggagactca
     3001 taaacaccgc cctttgcatt cggaataaat ttactaatag cactcccgat agttcctaaa
     3061 atacccccag aagaactgtt actaatgctg tcgaaaatcc cagtaattga agcctttaat
     3121 gcaattcgac taagatccga aatcacggaa gtagcgaaag aacgaaaatt tgccttgcct
     3181 gtcgtgacaa aatcacctag cgcatcggtc atcccatcaa acatctgagt cgtggttgat
     3241 tttatctgcg caccgatatc cttagtgtca tccagccagt tattgaatcc ctgggaggca
     3301 ccactaatcc agtctcctgc ctgaatatcg agctgctcgt ttatctggct gacaatttct
     3361 ttttctcgtt ccagagcatc attcagagcc tgcattttct cctgaaaaac atgatttgac
     3421 attccacggg atttatctgc atagtcacgt tcaagttgca gacgctgatt gttatattca
     3481 cgttcaatcc gcagtaattc ctgctggcgt tgctgatttt tatcgccaac cccataacca
     3541 gcaatctgaa tgtcataccc ctgttgacga ttatcaatcg aagcctgcaa tgaatcacgc
     3601 catgcagcta tttcggcgga ttccttgatt agcctgttat ttttttcaat cgcaacattt
     3661 ttctccatca acgcggttat ttcttcccgg tgtaagagaa gcgatttctg atcctgggtt
     3721 aatttcgatg acggtcgtga ctccaggtcg gcaatctgct gacgccactt aaccagttcc
     3781 tgttcagagg aacttaattt aacggtcaat tcagcttgtg aacttagtaa cgcattctgt
     3841 tgattcagat gatcaatcat tcgttgggca gcatcatccg aataaccttt cgcctttggt
     3901 tgctttggat ctttataacg ctcatttatt tgagcgataa gattactata ttcttccttt
     3961 gaatactgat tctttagctt ttccagtttt gcaagttcac tagctctctg ctgctcccgg
     4021 gtctgatatt gttttgaaaa agcatctgct ctctgtctga gttcaattcc ttcctgttgt
     4081 cgcttgttgt aatcgtttat tgatgaattt agtacgtcct gagcaatttt ttctgcttga
     4141 agtacgccca attgctgctt taatcgtgcc agctttttat tttgagctcc gccatctcca
     4201 atgccaccta acccgaaaac tccgggtcgg gtattttttt ctatttcatc aatttgacgg
     4261 agaacatctg agattttttg atcaagggag gcctcacggc caatatccag catggaatcc
     4321 catgcccatt ttgcggagtc tgcgacagct ttccatgcag tctcaagata accaagattt
     4381 tctttaatct ggttggtgcg ctggatcatt gaggatgagt atgcttctgt cgcaatgcgg
     4441 gcgacctcct gctggttccc ttcatcctgt agcgccttaa tctggttata agtcgcaagt
     4501 gtcagaaaat ggtactgatc attaagtttt gatatagcac tgacaggatc ctttgcaatt
     4561 tcattgaagt cattaaccag ttgttcggtt gatattcctg ttacttcgct cgtttttact
     4621 atcgctgtcg tcacttgctc cagcgaactg ctcgctacct ttcccgaacg cacaagctgg
     4681 tttaatactg ccgccgcagc gccagttgtc gaatcagccg cattcccggc acgttgagct
     4741 atatcggcca attgcccgct ggttgtcccc aactgatttc cggtaagaat aagagattta
     4801 ttaaattcgt cctgctcctg agagccttta tagtaagcca gccctaagac gccaacggct
     4861 gctgcggcca gggtaaaagg attaattaat cccagcacat aagaacctac acctttgatc
     4921 gccgggccaa tcccaccgaa catatctttt agctggccgc cctgctgcat taacaccata
     4981 aatggcgact gaccagtgga caacccaacc acaatatccg tcatttgtgc aggcaacatg
     5041 cgcatagcaa aagccgtttg ttttgccgac attccggttt tgcttaattg cgatcgagta
     5101 acctcaagct cactccgcat agcacgaagt tttccagaaa gctcctcata catttcagga
     5161 gaaagcatcc ccttagcttt tgcttcattg agctgtttct gttgttctac cagacgatta
     5221 aaagcagttc cgacaggatc aagttgagca atcagacgtt gcaaagcagc aacctgttca
     5281 tcatgtgctt ttgctgcttc tcgctctgcc tgagcctctc cggtaagctc tcgccgtgtt
     5341 tcctgtattt ttcggctata attctcaaac tgagaaccat ttattttccc ggatgcaaac
     5401 gcagcattaa gttcatcctg ctgttgttca agatttctta gcgccgcagc cagagggtcg
     5461 atcttgtcca gcattctttg aaaggcctga gcctgcgctt cctgctgggc ggcagcaagt
     5521 tttccggcct tctcggcttc tctctgcgct tgcgcaaccc cgctcaattc ctctgtggtt
     5581 tcattaagtt tacggacaag aaattcatat tcttctttat caataagccc tttatcaaaa
     5641 aatttcttta attcagaata gcgtcgaccg acagtatcaa ttgcggcacc aactggatca
     5701 atagctgctt ttaattttgc gagcgcgttc ttctcatctt ctgttgcctt agtcactttc
     5761 cctgcgctat ttgcagcagt ttccccagcc tgcgtcattt tgactaatga ggaggccaga
     5821 ttgtcagcat tatttttcgc tccagtgcta tcaataatta ttgcgagacg cgaggtttgc
     5881 tctgccattc attaaaactc ctgacaacaa aaaacccacc gaagtgggtt tcaggcgaca
     5941 taatagtaga tatagcgatt acgaggccac gcaatgcttt tctccaggag catcatcgat
     6001 ttaattaaag acaccatcac atctctgtaa cagagtgtac gtaattaaca actacacaca
     6061 ctgctcctga aaatactggt catccagtgc aaagatcact gcttcaaatt catcgcgctc
     6121 aatcaatacc ggatgagtgg ctaaatattc atttatctct gtcagagata aaggcaaagg
     6181 caccccagtc attccagcat aacgtcgggc acgggatatt accgaatagg cgtacaacaa
     6241 ttccttaagc accgggtcta tttctggttc cggtatcggt ggcaacctga gtttttctcg
     6301 cttccatctt gccttttcac ccttttctcc cccgaactcc gataaccacc gctgggcggc
     6361 tatggctttt ttatcgtatc ctgcttctgc tgctctttac cctgggcgat gctgactgct
     6421 tctgcaagga tctgccagta caactccgga ttctgcttaa gcagcgcgat ccctcgttct
     6481 ggcgtatatt ccagtgcaac ctcaacacca tcaaccagtt caccaacccc tttccagtct
     6541 ttcagcagat aacgagcagc attatcaatg agtaaatcat caacagaatc cacctcggaa
     6601 acctttgcaa tatcaaactc cttcgttccg acgtgcaaac tggcatccat tttctcaatg
     6661 tggcgacgga ttaatgcatt acgggagcga tactgatcgt tatcgctgct tgccaccaac
     6721 agttttaacc cgtctacagg ttttaagtcc ttcattgggg taaaccagcg ttcaccaccg
     6781 atgataattt tctgattaag aataaacatc cataacctca ttcaatgcgc ccccctgtag
     6841 tgcagtacca cagggggaat aacggaaaat caactaatcg cctcagcact ggcttttgcg
     6901 atcacggcag ccggggaggc tttttttctg gttatcgtag gtgcttcatc tgctgcggta
     6961 atgctcaatt gaacctggat aatgtcggta ttacccccat caggccattc acctgacacc
     7021 tgaaccttcg ggaaactgaa agtgtatgcc ccctctccat tcgaaagcgt gaagctgaac
     7081 ggaactgttt ctccagtcag tgttttactc cagatttccc acgcggcttt agaccatgaa
     7141 agcgtcaccg taccggacgg tgtaaaagta gtcggaatat ttgctcctgc ataaggcgag
     7201 ccagtcccga tacaacgctg tgtctggagt ttgttatcga actgaatatc aaaactgtcg
     7261 atacaaaaac cgttacctcc ggcaacacca ttcaggctta ctgctgaaac ctccttaaac
     7321 gaataacgta gctttcctgc actatccaca ggttcgcctt tgataaaatt cgtatcatcg
     7381 gcctttgatt cccagtccag tccggcaaag gttacggtcg ctgtaatatc gccgtcgtta
     7441 gggatctgca ttttccatga gccaacctgc gctcctctga cgacagaggc aattccgaca
     7501 tcggacgcat aggtcgccag agaaaatgtt attcgctcat tccccattgt cagagaatcg
     7561 cctgaccatt ccgcgccgaa acaggatgca agaaaatcat catgttgacc ccagcgaaat
     7621 ttggtaccaa catcaccgcc gacatccaca gtgccaggcg tcgccccctg agccatccgg
     7681 gagccaccaa tctcattatt ttcgccttta ttctgggtgg gttttactcc ccagcttgtg
     7741 cgttttaata aactccagtc accacttgct ggcgtagtgc ctgcaaccgt ctcccggata
     7801 aatgccgaga taacctttgc acctgaactc acaggagcct cctatgtcat taattgcgct
     7861 agagcgcgcg atatggaatt tgaagattaa gctggaacca gccattcttt tcgccaacgg
     7921 ctattgagga aacagcctgg taactgagac gatcgtcatc ctgaaactca aacagttccc
     7981 gcaatttatc ggctgtctca gtaatgagtt ttgaaccaga acctgcgggg acaaataact
     8041 gaataatgat tatccccgtg cgataaacaa tcggccccgc gccaatttca ttaactccag
     8101 cctgcccggg aatattactt aaccgcgccc agattaactt accggaagga tcgaacgttg
     8161 gcgcgttcgg atacaatacg tcttttccat caataatcgt ctgtgccgtc attctggaaa
     8221 taaccgtatt tctgatttca gtaaacgtca tttgtaagcc tgtaaaacac catgaaaagc
     8281 gttggcatac acgccagttg gcgcttgttg tgaatgaccg ttttcaagag cctctgcata
     8341 aggaaggtta ttctggatat aaataactcc gtaatttgca gctttcgaaa taaccccgat
     8401 cccacgctga acagcaatcg taccgttcgg atccacgtta tcagatatac caaaatcggg
     8461 atgctgtaac gacaccaggt tgttatttct gaaacgtccg gtatcaaccg gagcagcaat
     8521 atcaatagca gtaagaatct gaatagcgat gtagcgaatc ttcagcccta catcttcctc
     8581 aatcatcccg gcaaatattg acggttctat atcccatgcc tttgccattt acgctctcct
     8641 taactggata gagtaaactg atgcggaagg atctacactt gctgtaatta cctcgtatcg
     8701 ttgtaactgc cttgatacag gatcataaat ctcaataata tggtcgacag ctggtttatc
     8761 cgtaacctcg cataccagag cggttaattt aaggtcacca tgcaaaatat taatcccatc
     8821 aattctgccc agcttataac gtgtcaacac gcctcgcccg gtatagattg cggtggattc
     8881 gccgccagtt tccgtcacag gatcccagtg ccggtgcgta acgtaagaac cagaaaaatc
     8941 actcacggcg tccgctaaat cctcatcaaa agcagcggca acctctgact gaatctcttc
     9001 acgaagcccc attatccccc cctcacaacc ctgacttgtg agcgactaag tccgtacggt
     9061 ttcagcagtg ctatcgcaag ctgtaaatcg ggttcaagca atgcagtgct gtttgctggc
     9121 aactcagaaa atgatttcga tacactgacc ccgtcagccg acacggcttt actgataaca
     9181 acgccagaat catttttctg ctgaaacaac ttaccgaatg aggcaattct ggctgcatat
     9241 gctcccgcaa gttttacctc ttccggaata cgggatgggt taattttcag gttgaagcca
     9301 ttaagccagg cattagccat taaaacagct ttatttttag cgttctcact cgtccaggcg
     9361 ttcccaaacg cattatcaac gtcatcacag gtcacgtaag tgatcatgtg ttactcctga
     9421 gttttccagc ccatagcctt ccagttgtca acttcatcag gatgaacatt agcgatagtt
     9481 ggtgcgccgg gaaacatctg ataatcggtc accataacca ctaactcaat ttgcgttttt
     9541 tgtgcggctt cacgctgtgc tctttgctct ttagttaatc cggccatatg ccccccatta
     9601 aaaatggggc cgaagccccg tttgaatgtt taaccaagaa tcagacaacc atgtgccggc
     9661 ttcactgatg aaacacccca tgccagtcca acttcatagc gcacctggcg atactgacga
     9721 tacagtgcaa tctggaacgt aatccctgaa atcgggtccg taacattcat tacatcatca
     9781 gcattatcgc caccttccgg cattgccgga gtacgggatg ccagcagaaa cgcgttgcga
     9841 tcaaacgcca tatttgccgt aaaggcccca acaactgtga tggcagtatc atcggccaga
     9901 tcctgacgca gtccgggcgc tgcaagagta atcagattgc tggtcgctgc tgccacaaca
     9961 tactgattcg gatcaccagc gaacgtaaca atctgacctg cagaaatact ccccgaacca
    10021 gtatcaatgg aaataagaac atcgccttct tttttctctc cattcacgag ataaccagtt
    10081 gcagcaacct ttggcgctcg tttcacacct gccgaactgt gaatattgaa tccctccaga
    10141 cgccccaaca cgccctcacg cagtagctgt tcagtgccgg attcattcac tttaaacaat
    10201 acagactgtt ttccgcgcaa attagcaatg gcagtggagc caagcaccat ctgcagatcg
    10261 gttgtcggtg cgccgttatc ctccagaacc tgacgagcca gagcagcatc agaaagatca
    10321 tctttaacac caaacggcgt tgtccctgcg gttcccacgg cgcgggaagc accgaaatac
    10381 aacgcaccaa gatcagcctc aacctcgttt gcaagggcgc gaaaagcctg cttgaactga
    10441 tcagccagaa tggtgttgta agtccctgaa gggccaagag ccagttgttc ttcaccattc
    10501 catttaaccg gtgccatttt ggatttagta atttttacat caacagtgcc aatattttga
    10561 tcaccggtat tcggagctga cggccccggt acgatatctt cggttttcgc ctcaggcgca
    10621 actggcgcgg ttaccgtctg atcttttgct gcggcgtcag cttttgcgtt ttttgctacc
    10681 gcagggataa aacctacctg ctcacgggat acaacatcca gggcggtata aatagtcggg
    10741 atcaacccgg tcagggtatt tccagccata attaaatatt ccttaaaaat ttgcgtaatt
    10801 gtgaatagat ggagtaatga gctatccagc cctgacacca gctcccatcc ggaagctgac
    10861 aaatgtgtta atcaacgatt gtgataccgt ctttcaatgc gttttgctta cctgcaacat
    10921 ccagtgcatc aaaagcagag cgtttcatcg ttttctgacc aatatcatgc tgtgtcggac
    10981 gggagccgcc gccattgttg ccactggctt tcaggatgta gtctttctga gggtaatttt
    11041 cgacaaggaa ctccagcgcc tcatcaaact gcgccagttc gccaggcttc gcgcgggaat
    11101 aaattttgtt gccggaagcg tcataagcaa cgatcctccc ttcttccact ttgaatgcct
    11161 gcccgaagcg ggcttgtaat aaatctgccg ggatcgcaat tttatcggca atatattttg
    11221 aacccgcaaa actaccgcca atcatagaat cgtaaagctg cttctccagc atctgagagc
    11281 gttgcttttc ttcatctaat tgctgctgaa aatttttcgt aatttctgcc ttaacctggt
    11341 caacctgtcc cgcatcgagc agctttttct ggtcgatttt tgacagcatt tccagtgcct
    11401 cgatcgcctt cttcgggtct tcgatagcgg caaacttagc cagtttttcc tctgcagctt
    11461 ctttagccag gcgatgattt tttgcctcgc cattaagctc tgtaattttt tttgtcgcca
    11521 gcggtgcatc gaagccaatt tctttaccat cgtcgtgcac ataaactggc aggccagcag
    11581 tatcaatttc tgcgtattgt tttccgttaa tctcgaccgt tttcagtttc atattagtac
    11641 ctggtttaag tcttccgaca gttacgctgc tcaccatccg gatcgcagca ataaaaaagg
    11701 ccacccgaag gtagcctgtt gtaataaatg atttatttaa atccctgctt ttctgaatac
    11761 ctgtgcatca cgctcacgga gctgcttcag cgtcagccat tcgcctttat cggtgtaaaa
    11821 ttcatctggc gacataccac catcccgaat cagctttgct cgggtttccc ccacaatctg
    11881 tttttgtctg gtgtaaggct gacgcaaaaa ccattccctg taggttgtat ctccggccac
    11941 cacgccatcc atgctggccc gctcagccgg ggaaatatcg cgaacatcaa tacccagttc
    12001 cttcgctgat ttcagaatga acgtttccgt tgagcggcag cagaaatgaa tttttcccgg
    12061 cccctgcaaa taaggcacgc tgtgacctac aggtttatta ttcagcgtat atttgaggcg
    12121 atccctgatt cgacattgtg gcgtagtacg attatcaagc gttgataacc attgcttacc
    12181 cttaatcaaa tcattattcg cgctggcaaa actctcacgg gcagtagcag caagatgtcc
    12241 aaccgctgtt tttgcaatgc tggccgcatt agcccggctc atctgcaatg caccatcctg
    12301 aaatccctta ctgacatgtc cccgaatttt tcttgcgatc tgctcattgg tatcccccag
    12361 caaaaaaccc tgacgcactg tatttgttat gcgtctgagc cgatccgcct caagatctga
    12421 ggcccactca ctgagcagtc gtccctggaa tggtcgcgcc attgcagcgg cataaagtgc
    12481 atcaggagag ataccaacca gagggtgaac atcagcaaca aaatcaggta gcatagaatc
    12541 aaacagactt aactgataac cagcctcata aattgccagc tcgttcagct ctccggagag
    12601 actggtaaac atgctgttaa tagcagtgcg gttaaccttt ctgacgctcg ccagaagtga
    12661 ctccaggcgc ttaacggtaa aattacctgg atcgaggctg tccagtgcta tcagcaggcg
    12721 agcagtaagt tccgcatcgc tgtcattcag tattttcacc attctggcag ccacgccagt
    12781 gctatagcgg gatatccaga ctgcatgagc aattgattca tcacgcagcc gttcattcac
    12841 ggtttgcatc attgatttcc atcagcatta cactctgatt ttttaattca tcgatcactt
    12901 cctctggacg ggaatcctga tcgataaatt tcaacgcctg caacacccga accgcatcaa
    12961 tctgacgtat atcaccgccc tgacgcaatg actgaacagc cagcgcggag gatgagtcaa
    13021 acacctgggc agatacatcc agttcagtgc gcacatccac attgccaccg caactctctc
    13081 cgatccattc cgccattatc tggagaatat tatcgagggc atcttcgagg gagttcgcca
    13141 ttgtataaag cggcgagttt tcctgcattc gctcttcatt agtctgatca acagatttgg
    13201 tggatgtatt ttcagcacgc agaagtttag cgccggcatg acgcatctga ttttccagct
    13261 tctcaagtga tgtttcgcca gattctatcg ctgcgccact atgttcaaca tattcgaggc
    13321 cattttttgt tctgtcctca aaaatcgtag cggtggatgc accaaccgtc agttcttcat
    13381 tcctgtccag cccgtaggcc accagcaatg gaacgcgggt aacatgaaga atattgtcct
    13441 gctcgctctg gctttgccag tgcttgatat tcagcaagcc aagattaagc aatggcggtg
    13501 taccacgcat aaatcctgtt ttcttcgtgt acagtgttac cagaggaata tcatcacggc
    13561 tggtattcca tgactcatga agcgtccaga cagattcgcc attagtgcct tcgctacgtc
    13621 gataaatttc aactcgacgg ggcataatat ggcggatctg ctccaccttc ttctgcccga
    13681 aatcatcacc atcaataatg atgacctctt ttatacgcaa atcagtgaga acaactttcc
    13741 ctttttcaac tttcgatttc catccaataa cctggcgtgg attcagcatc gtaacgtacg
    13801 gacgaccacc ggtcgcgttt tcatcggctt ttgtacgaat ctctttcata tccgttcgtg
    13861 gatagtccac cagcgcatgt gccacaccat actgaaatgc gaggctgaaa aattgctgcg
    13921 cccacacatc cagtcggctc ccctccatgt cgatattttc tgcatattcc ctgatttttg
    13981 ccggcgtttc ctcactcaat actgtcggct ctgcaaatat gcgcccaata ttttgcttga
    14041 tgctttcttc atacacagga agtagcgtag ccacagacag gcgtttttta taagcgtctt
    14101 catcttcgtt aggccatttt gggagataat tttccccctg cctgcgcatt tcaagcgtac
    14161 cgcccatcag tgcgtcgtta atatcccacg cctccagcat atcgttatag tcgaggttgg
    14221 gtgttgatat atcagccata attaaatccg aagtgatgtg actcttccgg tcggtttgac
    14281 aatagggaat tgcttaacga tgaaataacc tccggcatca ttcgggtgat cattgccaga
    14341 ttttttatca ggctccccct tctcatccca gacctgttgc tccagagatt cggcatataa
    14401 cgggcaacgc ttcacattaa ctttatagcg acgctcacca ttggcattgc agaacattgc
    14461 attcattgag ttaacgcgat cttttactgg cgggttcgag ctgttcacca caacgttaaa
    14521 accagcctgc ttaagctggg ctatatccgt cgtacttgcg ttacttgatt ttctggaatc
    14581 cccggaagca tctggataaa tataaatttc cctcactttc cggtaatcat tcccatcata
    14641 cagccagaag cgttctttaa tgatgcggat catatccggc gtatcgtagg cattgatgat
    14701 ttcagttacc gcacatggaa gccccaaacg cagcacatgg acgattcccg ccatctttcc
    14761 aacgttaaaa tccatcccaa tataaatcgg ctcccctggc tgctccactt cttcgcaatt
    14821 attcagtttc cggtcaaact gatggtaaac agtaccactt gtcaggttag taaactgtcc
    14881 tcgaagatag gctttaatca gctctggagg gtatgattca agaagcgaag gaatgtaatc
    14941 tgctggcagg ttcttttcat tatcgaaagt agatgcctgc accagaccat acagtgaggc
    15001 cagctctgtt ttttcacgca cggctttaac aaactgctcg tagacaaatt tgaatccttc
    15061 cggcgtggtt gtaacgtcaa taccgttgcg aagtccatca atcttataac gcatacgtgc
    15121 aattatcttg cgccacgccg ttctggcttt ttccttcggc aaaatgtcca gttcatccac
    15181 caacgcatta ccaattttga aaccgacgat cgtttgtggc ttctccatcg atctgcagat
    15241 agtggttccc cgatactggc gtccgtaata aaagtgaacc tctttatttc cctcattaat
    15301 ttttacgttc aatccccagt cagcagcaac ttcttccact gtagggtaaa aaatatcgcg
    15361 aatttgggga tacgttggcg caaaatatcc ctgatttata cctggatgct cccaaatccc
    15421 cttgcatatg ccgccacacc caacccatgt tttgcccgag ccaaaaccag caatataggc
    15481 tttaaattta tggggcattg aaagaaatcg cgcctgaggc acattaagcg tcggagagat
    15541 catcttcatc actccttact ctggcatcaa ccacattaat attgatcgcc acaggctggg
    15601 gatgttcatt atcttccacc gtttcgatct ctttgcgcag cttctggttt tccattctgc
    15661 gccgttcaat ttccagttcc cgtagccgct tatctgcaca taaagccccg ccagcagaaa
    15721 acaaacgcaa caattcacgc cgggcggcag ccttatcctc cagcaggatc tcaacgccga
    15781 attttccgaa ttttgcccct gcatataatt gccgcgcatc cccatcaagc agagtggtat
    15841 cagccatata aagctgtccc gttccctcac cgcagcactt cgggcagtcc ggattgggta
    15901 tggcgttatc aacaaagccg aggcctccat attccggttc gggtttgcca tctctggaag
    15961 cctgcgccgc tgccttgtcg aattctgcta tatcgcgcca ctggtagaga tgattctcgc
    16021 cccagcaata acggcagtta acacggcgaa attgtgcaag ctgattgggg tcggccagga
    16081 caatagccat caactgactc actagtaaat ccaggtctgc ggtatagcgt ttctggtact
    16141 gattgcggaa gtagttgata gcgcgaaaaa ccctggcatt tctaagcata cgactggcgt
    16201 tgctgttagc tgtcgcacct tgcccctcat aaccggctag tcggtacgcc tctgtcggct
    16261 ttttaccctg agcaacaagc atcgcaaact tagcctgctg gtcagaaatg ccgaattcat
    16321 cgggacagaa cgaaaattcc tccgcgtcgc cctcattcag gcccgcatcg aatactggct
    16381 ttttttcctg agatttttcg ttccgctttt gtgcagtctg cgcagatttt ttctgcgcac
    16441 ttttttgcgc agttttgcac atttctgtct gcgcattttt cggagttttt ttgatgtaac
    16501 gacgggctgt tgcgtaattc agtccccttg cttcacacca tgccaccgga gatataccgg
    16561 agcgggtgta ttcagcaata tactcctgct gcaacgcccc ccagtccggt ctgctcatca
    16621 gttagtcctg atttttatcc accctgagta gttcgcgcag agcaaaggca tccccttttc
    16681 tggcaagctt aaacaatgcc gctcgtaact cggcttcacc tttcgctctg cccttacgga
    16741 tggccgcata aaaatctgtc attgcttccc gattttcttt cagtcggttc agatcaacat
    16801 ccagaacgtc agcgatttgt tgtgcagtca tccggcacgc tgccagagac tcgactttcg
    16861 aatacggaat catttgtcac ccccattgat atgcagggtg tcttcttcct gtatttttcg
    16921 tgaaggattt ttactgcagc gttgttccag gtgacctgat ggtgaatgcg tttatgactg
    16981 gcacccataa gtgagatttt tacgcacgac ggcgcataca tgacggagta aaaactttta
    17041 acgtaggttc cggaatccag atacagctcg gtcattccgc cgctgttttt ctgcgtctgt
    17101 ttctgcccta actggacagc accgatcgtc ataaacaatt caccacagcg accgagattc
    17161 gtgtacgtat tcacatcctc gttaatgcgc cccatgaatg agaacggtcg atcaaccgaa
    17221 cagataaagc tgttcattgc cttgcgtttc acccacgaag catggccgcc attgtcacca
    17281 agaaaatccc cgccctgcga catagcgatg gaaagagcag gtattgattc gtagtacgcc
    17341 agcatttcag aaaggatcgc atccagtttc cttatcggaa aataggcctg gtcatagttg
    17401 cgatccaccc gaaactggaa ctcgtgataa tcatcatcga gctgaatgaa gtatttacac
    17461 ccgaccagtt ttgccaggtc gaaacaggca ttacgggcgt aaaaaattga gcggcggtca
    17521 cagaaattat cggcttcgtc aaaacgactg gcgatatcgg ctttggaaaa caccagcacc
    17581 tgttcaccaa attcagccat gtactgattc cgtgtcttat cttcatcatc aacaacgata
    17641 aaaattttcc cggtatagcc agcacgacgc aacgtccggt aagtcagaac tttgtccggt
    17701 cgcccgtgag tcagaataaa ggcgcaaaaa tcatcacgca tattcctcct cctcaccacc
    17761 atgcatgatc tccaccatgc gctgcgtcat ccggacaaat ccattttcaa tagcctgctg
    17821 ataatcaatg atcaccagcg ccgactcctc gaaaaaacac tgaatttcag agggggcgtg
    17881 agcgtaatag tccgcaattc tgctgaaatt aaacaccgtg tgacgttctg ccgcacacag
    17941 gaggaatttc tcaatatcag gctcaaggga cgccgaacgt atccggctga tcagctcctg
    18001 agttttcgta tcgtcgtaca gttcactgat atccggttta ccgcccgacg gctcataaac
    18061 aggcgtatca attttcgtcg tatacggctc ctcctcattt cctgtaccgg gcaaaacatc
    18121 cgtcaacagt tcatcaattt ctgtcgggat gaagcctgtc agggagacat caaaatcagc
    18181 attgattagg tccgacagct ccatccgcaa cagatcttca tcccagccag cattcatcgg
    18241 caggcgatta tctgccaggc ggtacgcctt tttctgctca tccgtcaggc cagacagaac
    18301 aatgaccgga acagaatcca ttttgagcat ttcagccgcc ataacacgac cgtgacccgc
    18361 aataatttcg cccttttcgt caatcagcac cggattagtc cagccgaatt gcttaatact
    18421 ttctaccagt tgtgccacct gctcagggct gtgcgtcctg gcgttgtgcg catacggtga
    18481 caattcttgt aatgggcgat agactatctt taatttctcg ctcatacagc cttgctttat
    18541 gaataaaacg caccccagca gccagtgcta ctggggacgg aggggttgct ggtaaagtta
    18601 ggtattggat caatgagtga gtcaacataa tattaaactc acaattataa atcagccata
    18661 tattaggagc gccaaaaaaa acctgaaaac aatataataa caggataaat ttcaaggcga
    18721 ccaagaatca tagctatgca cattaaacat tttgcaatgt cattaagcac tccgaatgac
    18781 gatgcagtag ccccaaaacc taatcccata ttattaatac atgcagccac tgttgcaaat
    18841 gatgtaagaa aatcatatcc cataccattt aacaccagta taaaaaacac cgtgaagaga
    18901 gtataaagaa aaaagaaact ccatacagac ctcattacac gatctgtaac tatcttccct
    18961 cctacattta cactcaacaa cgctctggga tgagaaagct gatttatctc gtgtttgctt
    19021 tgtttgaaaa gtataagaaa tcgaagtgac ttaattccac cacaagttga acctatacat
    19081 cccccaaaga aacttgacaa cagcaaaaac actatcgtgt gcgtgggcca acttgcataa
    19141 tcctgcgtag ctaaaccatt atcagtgagc atggagctgg caagaaaaaa cgaatgaata
    19201 aaacttccat gcaagtcata catacctata tgccagacct ggaaagaggt aacaatgatc
    19261 acccctaagg ctattaacag aaagaaacga agttcaatat ctctgattaa aggttttatc
    19321 gttttcctgc taataacaat ataccaaaga gtgaagttga aagccgatag cagggaaaaa
    19381 gaaccagcca ccagctcaac caaatagtta ttaaaatatc cgatactctc gctatgagtt
    19441 gagaaaccac caagcgaaac tgtggaaatc ccgtgacaaa tagcatcaaa caaaggcatt
    19501 cctgcaagtc tataacagac aatacaagca atacctaata aagaataagt tatccacagt
    19561 gtccgtgacg tatcggccag gcggggagtg agtttgtcat ccttaaatgg ccccggcatt
    19621 tctgactgat aaagctttgc accaccaata cccaataatg gcaatacagc aaccgccaga
    19681 acaataactc ctaaaccacc tataaaattt aactgtgacc gatagtacaa atatgcccga
    19741 ggtaatgaac taacatcatc aattacagtt gctcctgttg ttgttattcc agaaacccct
    19801 tcaaacagag catcaatgaa cgttaaatta agttctgagt caatccataa agggaatgca
    19861 ctaataacag aaaacaaaat ccaaaacatt acaattataa taaacccatc acgggtacgt
    19921 aattgaatgc cagatttctt agttgtatac cacgctccgc caccaatgca aaaaaatata
    19981 acgaaagtta taaagaaaac gaacaggctt ttttctttat aaaacaatgc tacaaccatt
    20041 ggtggcaaca ttgaaagact atagagccaa accaggaacc cacacatatg agtaacaact
    20101 cttacatgag atgtattcat atctaaatat tctttcaatt ataaccacct tgctgcaata
    20161 tta
//
LOCUS       DAPHCV010000092        19756 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.092, whole genome shotgun sequence.
ACCESSION   DAPHCV010000092 DAPHCV010000000
VERSION     DAPHCV010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.092"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..94)
                     /locus_tag="RH033_003392"
     CDS             complement(<1..94)
                     /locus_tag="RH033_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992532.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQT"
     gene            148..2133
                     /gene="pdeR"
                     /locus_tag="RH033_003393"
     CDS             148..2133
                     /gene="pdeR"
                     /locus_tag="RH033_003393"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HDV2992533.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPERTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGISLSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            2281..2454
                     /locus_tag="RH033_003394"
     CDS             2281..2454
                     /locus_tag="RH033_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992534.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            2544..3293
                     /locus_tag="RH033_003395"
     CDS             2544..3293
                     /locus_tag="RH033_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDV2992535.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            3562..3780
                     /gene="osmB"
                     /locus_tag="RH033_003396"
     CDS             3562..3780
                     /gene="osmB"
                     /locus_tag="RH033_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HDV2992536.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(3906..4232)
                     /gene="yciH"
                     /locus_tag="RH033_003397"
     CDS             complement(3906..4232)
                     /gene="yciH"
                     /locus_tag="RH033_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HDV2992537.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGIIEIQGDKRDLLKSLLEEKGMK
                     VKLAGG"
     gene            complement(4232..4969)
                     /gene="pyrF"
                     /locus_tag="RH033_003398"
     CDS             complement(4232..4969)
                     /gene="pyrF"
                     /locus_tag="RH033_003398"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDV2992538.1"
                     /translation="MTLTASSSSRAVTKSPVVVALDYHNRDAALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAARFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(5160..6329)
                     /gene="lapB"
                     /locus_tag="RH033_003399"
     CDS             complement(5160..6329)
                     /gene="lapB"
                     /locus_tag="RH033_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HDV2992539.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMALLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESMQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(6336..6644)
                     /gene="lapA"
                     /locus_tag="RH033_003400"
     CDS             complement(6336..6644)
                     /gene="lapA"
                     /locus_tag="RH033_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HDV2992540.1"
                     /translation="MKYLLIFSLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVAPHSSAAKE
                     "
     gene            complement(6793..7557)
                     /gene="pgpB"
                     /locus_tag="RH033_003401"
     CDS             complement(6793..7557)
                     /gene="pgpB"
                     /locus_tag="RH033_003401"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HDV2992541.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAIMLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            7727..8317
                     /gene="ribA"
                     /locus_tag="RH033_003402"
     CDS             7727..8317
                     /gene="ribA"
                     /locus_tag="RH033_003402"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HDV2992542.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(8381..11056)
                     /gene="acnA"
                     /locus_tag="RH033_003403"
     CDS             complement(8381..11056)
                     /gene="acnA"
                     /locus_tag="RH033_003403"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HDV2992543.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIYALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(11206..11301)
                     /locus_tag="RH033_003404"
     CDS             complement(11206..11301)
                     /locus_tag="RH033_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992544.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(11415..11582)
                     /locus_tag="RH033_003405"
     CDS             complement(11415..11582)
                     /locus_tag="RH033_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992545.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQQKQ"
     gene            complement(11585..11749)
                     /locus_tag="RH033_003406"
     CDS             complement(11585..11749)
                     /locus_tag="RH033_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HDV2992546.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            complement(12044..13018)
                     /gene="cysB"
                     /locus_tag="RH033_003407"
     CDS             complement(12044..13018)
                     /gene="cysB"
                     /locus_tag="RH033_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HDV2992547.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(13228..15825)
                     /gene="topA"
                     /locus_tag="RH033_003408"
     CDS             complement(13228..15825)
                     /gene="topA"
                     /locus_tag="RH033_003408"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDV2992548.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGDALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            16205..16456
                     /locus_tag="RH033_003409"
     CDS             16205..16456
                     /locus_tag="RH033_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HDV2992549.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(16492..17541)
                     /gene="sohB"
                     /locus_tag="RH033_003410"
     CDS             complement(16492..17541)
                     /gene="sohB"
                     /locus_tag="RH033_003410"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HDV2992550.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDTHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLNIDQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            17761..18519
                     /locus_tag="RH033_003411"
     CDS             17761..18519
                     /locus_tag="RH033_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HDV2992551.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSNVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            18516..19106
                     /gene="cobO"
                     /locus_tag="RH033_003412"
     CDS             18516..19106
                     /gene="cobO"
                     /locus_tag="RH033_003412"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HDV2992552.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGIVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADPSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(19158..19466)
                     /locus_tag="RH033_003413"
     CDS             complement(19158..19466)
                     /locus_tag="RH033_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992553.1"
                     /translation="MNDVQNRRILALLDMLYNTLGNKTTCNVCHKYFILILTVMNNLT
                     INYGTETSPELSRISYEDANALITELILFIEDVDAENHEAYQGHLNEITRILNFAIAA
                     "
     gene            complement(19477..>19756)
                     /locus_tag="RH033_003414"
     CDS             complement(19477..>19756)
                     /locus_tag="RH033_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992554.1"
                     /translation="EFNTLKNRKEFLSSVINVNETRLQASAPAMPAIEALNAFIDKGD
                     YLKSLYVNKTRTAAEQKRLNEILRAQVISGLKASEGLAKLLIASGIML"
BASE COUNT         5001 a         5056 c         4621 g         5078 t
ORIGIN      
        1 tcgtttgaaa actggcgttt ttccaaaact ggattacaaa taaatatata aaaaaaacaa
       61 tgcgtttagc cgccgttaat ttcatgtaag tcatgttgtt ccttttattt aaccgcggac
      121 tccgctgtta accggaggat atgcatcatg aaaaccgtta gggagtccac aacgttgtac
      181 aactttctcg gatcgcacaa tccatactgg cggttaacgg aaagcagcga tgttttgcgc
      241 ttttctacca ccgaaaccac agaacctgaa cgtacgttgc agttatctgc cgaacaggct
      301 gctcgcatca gggaaatgac ggtcatcacc tccagcctga tgatgagtct gaccgtcgat
      361 gaaagcgatc tttctgtgca tctggtagga cgaaaaatca ataaacggga atgggctggc
      421 aacgcgtctg catggcatga cacaccagca gttgctcgtg atttatcaca cggactttcc
      481 tttgctgagc aggtagtttc tgaagcacat tccgcaatag tgattctcga cagccggggg
      541 aatatccaac gcttcaatcg gttatgtgaa gattacactg ggttgaaaga acacgacgtc
      601 attggccaaa gcgtgtttaa actgtttatg agccgtcgtg aagctgcggc atccaggcgc
      661 aataaccgtg tattttttcg aagcggcaat gcatatgaag tcgaactgtg gataccaaca
      721 cgtaaaggcc agcggctgtt tctgtttcgc aataaatttg tccacagcgg cagtggcaag
      781 aacgaaattt ttttaatctg ttccggcacc gacattaccg aagagcgccg cgctcaggag
      841 cgactgcgta ttctggcaaa taccgacagt atcaccggac tgccgaatcg taacgcaatg
      901 caggagttaa tcgatcacgc cattaatcag gcagataaca ataaagttgg ggttgtgtat
      961 cttgatttgg ataattttaa aaaggtcaac gacgcttatg ggcatttgtt tggtgaccag
     1021 ctattacgcg acgtgtcatt ggccatttta agctgtctcg aacatgatca ggtgttggcg
     1081 cgtccaggtg gcgatgagtt tctggtactg gcatccaata cctcacaaag cgcgctggaa
     1141 gcaatggcat cacgaatttt gacccgctta cgactcccct tccgcattgg tttaattgaa
     1201 gtttatacca gctgttcagt gggtatttca ctctctcccg aacatggttc ggacagcgcg
     1261 gctattattc gtcacgccga cacagcaatg tacacagcga aggaaggcgg acgaggacaa
     1321 ttttgcgttt ttactccaga aatgaatcag cgggtatttg aatatctctg gctggatacc
     1381 aacttgcgta aagcactgga aaacgatcag ttggttattc actatcaacc gaaaatcacc
     1441 tggcgtggcg aagtacgcag tctggaagct ttagtacgtt ggcagtcacc tgaacgtggg
     1501 ttaattccac cgttggactt tatttcctac gccgaagagt cagggctaat tgtgccttta
     1561 ggccgttggg tgattctcga tgtcgtacgc caggtggcga agtggcggga taaaggcatt
     1621 aacctgcgag tggcggtaaa catttctgca cgtcagctcg ccgatcaaac cattttcacc
     1681 gccctgaaac aggttctcca ggaactcaat tttgaatact gccctattga tgttgaactg
     1741 acggagagct gcctgattga gaatgatgaa ctggcacttt ctgttattca acaatttagc
     1801 caactaggtg cgcaagtgca tctggacgat tttggtaccg gctactcttc actttcgcaa
     1861 ctggcgcgct ttccgatcga tgccatcaaa cttgaccagg tttttgttcg agatattcac
     1921 aaacaacctg tctcgcagtc actggtccgg gcgatcgtcg ctgtggccca ggcattgaat
     1981 cttcaggtga tcgccgaagg tgtagagagt gcaaaggaag atgctttttt aaccaagaac
     2041 gggatcaatg agcggcaagg atttttgttt gccaaaccga tgcccgccgt cgccttcgaa
     2101 cgttggtata aacgctatct gaagcgcgca taagtagatg aatatatata cttagcgata
     2161 tattccgtaa ttatatatcg tattcccgga tgataattag ccgttgctgt taaacacttc
     2221 tcttttagcg gcaatttttg cattattcat tttccccctt ttgctataag gatgtccttg
     2281 atgtctgaat ttgatgccca gcgcgttgcc gaacgtattg atattgtgtt agatatcctt
     2341 gtggcaggcg attaccactc tgcgatacat aatctcgaga tacttaaagc agaactcttg
     2401 cgccaggttg ctgaatctac gccagacatc ccgaaggcac cgtgggagat ttaacaatcg
     2461 tgccttcagg ttttcctatt tgaggatgcc cggcgcgtca gtattttttt atttagtatt
     2521 ataacgttat aagaattaca gcgatgaatt cccgacaaca aactattcta cagatggtca
     2581 ttgaccaggg tcaggttagc gtaaccgatc tggcaaaagc cactggagtt tctgaagtta
     2641 ccattcgcca ggatctcaac accctcgaaa aactgagtta cctccgccgt gcacatggct
     2701 ttgcagtatc gcttgatagt gatgacgtcg aaactcgtat gatgagcaac tatacgctga
     2761 agcgtgaact cgccgagttt gccgcgtcac tggttcaacc gggcgaaacc atctttatcg
     2821 aaaatggcag cagcaatgcc ctgctcgccc ggactctggg cgagcagaag aaaaatgtca
     2881 ctatcatcac ggtcagcagc tacatcgcgc atttgctgaa agacgcgcct tgtgaagtta
     2941 ttttgctcgg tggcgtgtac cagaaaaaaa gcgaaagtat ggttggcccg ttgacacgcc
     3001 agtgcatcca acaggtacat ttcagcaaag catttattgg tattgatggc tggcaacctg
     3061 aaactggatt caccggtcgc gatatgatgc gtaccgatgt ggtcaatgcc gtgctggaaa
     3121 aagagtgcga agcaatagtc ctgactgaca gctcgaaatt tggtgctgta cattcatact
     3181 ccatcggtcc cgttgagcga ttcaatcgcg tgattaccga ttcgaaaata cgcgccagcg
     3241 atctgatgca tcttgagcac agtaaactca ccgttcacgt cgttgacatt taaccatttc
     3301 ccttctctac ggatgatttg cagtttggca aatcatccgc tctaagatga ttcctggttg
     3361 ataattaaga ctatttacct gttattaaca ctctcaagat ataaaattat tatcagcgat
     3421 ataacaggaa gtcattatca cctgcgtgat ataaccctgc gcgcgagcgg atttcacgga
     3481 ataatttcac caggcttatt cttagctatt atagttatag agagcttact tccgtgaatc
     3541 ataaattcag gagagagtat tatgtttgta acgagcaaaa aaatgaccgc ggctgttctg
     3601 gcaattaccc tggcaatgtc tctgagtgcc tgttctaact ggtctaaacg agaccgtaac
     3661 accgcaattg gcgcgggtgc aggtgcatta ggcggtgcag tactaaccga tggcagtacg
     3721 ttgggtacat taggtggcgc agctgtcggt ggtgttattg gtcaccaggt cggtaaataa
     3781 tcgtaaataa atgtcgatta tcgactcgca attataatac gcttgattct gttcagtacg
     3841 aaataactga ataatacaca cagtaaaata ttcgaaagcc acggatatat ccgtggcttt
     3901 ttatgttaac cgccagcgag ttttaccttc atccctttct cttcaagcag tgattttaat
     3961 aaatcgcgct tatcaccctg gatttcaata attccatctt taactgcccc gccgcagccg
     4021 cattttttct tcagttccgc tgccagtttt gtcagttcgg catcatcgag atcgacaccg
     4081 gtaatcaggc aaacgccctt acctttacgt ccactggtct gacgctgaat acgcaccaca
     4141 ccgtcgcctt tagggcgtac aggggccgct tttggctcat caatacgtcc ggtttccgtt
     4201 gagtagacca gacggctgtt ggaatcactc atcatgcact ccgctgtaaa gaggcgttga
     4261 tcgctttcag cgtctgcgct ggatctaccg attgcgttac cgggcgacca atcaccatat
     4321 aatcaacacc agccgccaac gcctgttctg gcgtcataat gcggcgctgg tcaccagctt
     4381 cactcccctg cggacgaatg cccggcgtaa ccagtttgaa ctcctgacca aatacctgtt
     4441 taaagcgcgc agcttcctga gcagaacaca ccacaccatc aaggccacat ttttgcgtca
     4501 gtgccgccag acgttctgca taatctgcag gtgacagtgt cacgccaaga tcggccaggt
     4561 cgctggcttc catgctggtc aatactgtca cagcaatcaa aagcggtgca tctttgccaa
     4621 acggcaccag tgcctcacgc gctgcggtca tcatacgcgc cccaccggag gcgtgaacat
     4681 tcaccatcca cacgcctaag tcagctgcag cagcgacagc gtgcgctgca gtgttgggaa
     4741 tatcgtgaaa tttcaggtcg aggaagatat caaaaccacg ctgttgaagt tcgcgtacaa
     4801 attgaggtcc aaacagcgta aacatctctt tgccgacttt cagacgacaa tcgcgtgggt
     4861 cgatcttgtc gacaaaggcc agcgcggcat cacgattatg ataatcaagg gcaacaacca
     4921 caggagattt cgtaacagcg cgggaagaag atgaagcagt taacgtcatg accagacctt
     4981 cttgatgatg ggcaccggaa gtggcgcgaa acaggtaaac ggcgagcatt ctacccgtgg
     5041 aaataaaaaa ttaacagttg cgatttctcc cctggcaggc ttttctgcgc cggagttccc
     5101 ggagcacaat gaacatcaat tattagcatg ttgtaactaa agtcggattt ttttaaaaat
     5161 tacaggccat caagaccgcg aatcggttta atggttgacc aggcccgaca agacggacaa
     5221 tgccagtaga gggtgtatgc ggtaaaacca catttctggc agcgataacg aggcttacta
     5281 cgcaccttct cgccaaccat gtcacgcagc accatcagac tctctttggc acgcccttct
     5341 tccgcttcat ttaagtggta atccattagc ttatggaaca cacgcatggt cggatgacgc
     5401 tgaagctgac gcgtaatata gacctgtgcg gcctcactac cgtcgcgcgc ttcgataata
     5461 tccgcaagca tcaattcagc atcggcaccg gtgttctctt ccaccgcgcg ctgcaggaat
     5521 tctgcccatt cggcagtttt acccaactgc tgatagcagg tttgcaacat ttccagcgtt
     5581 tcgctgacca gttctctgtc ctgagatatc acgcgttgca tactttcgac ggctttggcg
     5641 tattctcctt tcgccataaa tacgcgtccc atcatgatgg atacgcgggc gctgttttta
     5701 tctgccgccg cacctttttt taacaaggcc atggcgcgat cgagatcgtc gctggccata
     5761 tgctgcaggg ctaactcaca gtagaaatgg gcaatttcga cgcgctgctt atctttaccc
     5821 aacttcacca gacgttcggc aacatcaatt gctttctgcc actcgctggt agcctggtag
     5881 atttgtagca actgttgcag cgcgccaatg cggaagtcag tttcatcggt cagctgattg
     5941 aacatgtctt ccgcgcggtc gtataacccg gcggccatgt aatcacgccc cagttgttga
     6001 atcgccaaca gacgctgttc ataggtcagc gaggcgcttt ccattagggt ctgatggatg
     6061 cgaatagcgc gatcaacttc gccacgcgaa cggaacaggt ttcctagcgt aaggtgggct
     6121 tcaacggtac ccgtatcctc tttaagcata tcgagaaaca ggtctaccgc tttatcctgt
     6181 tgattactaa gcaggaagtt aacccccgct acgtaatcac gcgacaagcg gttggcttca
     6241 tcttgcttgt tttgttgcgc acttctgcgg cccatatacc agccataggc agcggctaca
     6301 ggcaaaagca gaaacaacaa ctccagcata gaaagttatt ccttcgccgc tgacgaatgc
     6361 ggcgctacag ccacgtcagt cgcgggtgaa agctggtttt ccagtcgctt tattttacgt
     6421 tcagcgcgcg ccagggaaac acgaactcgc agccagaaca gaccgcaaat cagccaaccg
     6481 atagcaaacc ccgcagcaaa caataccgcc agcaatgtgg aaatacggta ctccccttgc
     6541 gctaacagat aattaaacgt cacctgttga tcgttctgcg cacctaacgt gaccgaaatc
     6601 acgaagatcg ctaacaccag tgagaaaatg agtaaatatt tcacattact tcccgttatg
     6661 tggttagagc gaataaatta tggtcaactt accgcaatag ccctttaaag taccattttc
     6721 gcgccgtctg cgaaatggca tccacgcgca ttgatgatga tttaagggct aaattcggaa
     6781 aatcaaccag cgttaacttt cttgttctcg ttgtgcaatt tcgcgatttt cttccgcagg
     6841 tggtgttaat ggcccacaaa ttcgttgcgc aagccacgtt gccaccgcca ccagcgccca
     6901 cgaaatcaac gtagctacca ccagatcgcg tggccaatgc atcccgagca gcaggcggct
     6961 tcccatgact cccgttgccc agaccaacaa gatagcaatg gttaacgttc gccgacgcgg
     7021 ccacagcaaa ccaacggcca gcagcgccca actggcagca aacatcgtgt gaccggaagg
     7081 aaaggcaaac cccgtctctt tctgccagtg tgaacgcaaa tattgtggga tatttttctc
     7141 ttcagccaac tgttctttca ccagatttcc gcgttctgct cgctttaaag tgtagaactc
     7201 atcaaccgga atatgatgtg ttttttccag ccagataaca aaaggtcgtg gttcctggac
     7261 tttgtctttg atccatgatt taacgccttg tcccacaaga attgccgccg ccagaatggc
     7321 aaataacata atggcagcct taatgcgaaa acgcagacac cagagaaacc aaccgaataa
     7381 aatcaaatgt gtaatgacgc cccagggctg ggtgacagtt tcagtaaccc aaaaagccgc
     7441 ttttagtagc caactttgtt ctccaggttg ccaacgccag ccagaaatcc atacggctac
     7501 tggcataaca agcaatagtg cagctcccac tgcggtacgt ctggcaatcg aacgcatggc
     7561 ctctcctttt tgataagtcc cacaatcata actgaaaatg ccagttccag gaaaaattga
     7621 cagatttgtg ccattccgtg aacgatcgac gcgtcgtgat taggtgaacc ccttctcgtt
     7681 atggcaaaat aagccaatac agaaccagca ttatctggag aatttcatgc agcttaaacg
     7741 tgtggcagaa gccaaactgc caaccccatg gggcgatttc ctgatggtgg gatttgaaga
     7801 actggcaacc ggacacgatc atgtcgcgct agtctatggc gatatctccg ggcatacccc
     7861 ggtacttgcg cgcgtccatt ccgaatgtct gaccggtgac gccctgttca gcttgcgctg
     7921 cgattgtggc ttccagctcg aagcggcact gacgcaaatt gccgaagaag gccgtggtat
     7981 tttgctgtat caccgtcagg aaggtcgtaa cattggtctg ctgaataaaa tccgcgctta
     8041 tgcactgcag gatcaaggtt acgatactgt agaggctaac caccagttag gcttcgccgc
     8101 tgatgagcgc gacttcactc tttgcgctga tatgttcaaa ctccttggcg tcaatgaagt
     8161 ccgcttgtta accaataacc cgaaaaaagt cgaaattctg accgaagcag ggattaatat
     8221 tgttgaacgc gtaccattga ttgtaggtcg taaccccaat aacgaacatt atctcgatac
     8281 caaagccgag aaaatgggcc atttgctgaa caaataaccc tcttgcattg tgtaattcat
     8341 ttgcttgccg gaagcaaaat aaccggcaag caaatacttg ttacttcaac atattacgaa
     8401 tgacataatg caaaatgccg tcgttctggt agtaggtcaa ctccgtcgcg gtgtcgatac
     8461 gacaacggca gggtacgact tcctggctac catccgcgcg cgtaagcgtc accggaaccg
     8521 tcgcgccggg ttgtaggttt tgcagatcgc caatatcaat cttctcttcc ccggttagcc
     8581 ctaacgtttt acgcgttacg ccttgcggaa attccagcgg caggatgccc atgccaatta
     8641 aattcgaacg gtgaattcgt tcaaacgatt cggcaatcac cacacgaata ccaagcagac
     8701 gcggaccttt tgccgcccag tcacggctgg agcctgatcc atactctttc ccggcaatca
     8761 ccgccagcgg cgtttgctcc tgcttatagc gcatcgcggc atcatagata gagacaacat
     8821 cgctgtcagg taaatgccgc gtcatccccc cttcaacgcc aggcaccatt tcattacgga
     8881 tgcgaatatt ggcgaaggtg ccgcgcatca tcacttcatg gttaccacgc cgcgaaccgt
     8941 aggagttaaa gtcttttcgc tcaacacccc gaccttgtag atatcgaccc gccgggctgt
     9001 cgggcttaat actgcccgcc ggagagatat ggtcagtggt gactgaatcc cccagcattg
     9061 cgaggatccg cgcaccgtga atatcttcca caggtgctgg tgttgcctgc atttcatcaa
     9121 agaatggcga taagcgaata taggttgaat cttcctgcca accgtaggta tcggatcgtg
     9181 tgacgttaat ttccttccac tctgctgtgc cttcaaaaac ttctgcgtac tctttgcgga
     9241 acatttctgt ggagacttgt tctaccgcac gggcaatttc ttgtgccgag ggccagatat
     9301 ctttcagata taccgggtcg cctttacgat cgtgaccaat aggctcagaa gcgaggttaa
     9361 tattcatatt tcccgccagc gcataggcaa ccaccagcgg cggcgaggcc agccagttag
     9421 ttttaaccag cggatggata cggccttcaa agttacggtt gccggacagc accgcaccga
     9481 cggttaaatc gccttttttg attgccgttt cgataggatc gggcagcggc ccagagttac
     9541 caatacaggt ggtacaaccg taccccacaa ggttaaaccc cagttcgtcg agatacggtg
     9601 tcaatttcgc ttttgccaga tagtcagaaa caactttcga gcctggtgcc agcgacgctt
     9661 tgacccacgg ttgccgcttg aggcccagag ttacagcttt tttcgccagc aagcctgcgg
     9721 ccatcagcac acttgggtta gaggtgttgg tgcacgaggt tatcgcagca atgaccaccg
     9781 cgccatcagg taactgatac tgatgtccgt tcataacata atcgaccggc tggcgatctt
     9841 tatgcgtggc attcacttcc agttcgttac tggcggcaaa tgcttttggt acatcgggca
     9901 gtgcaacgcg atcctgtggg cgtttaggcc ctgccaggct cgcttcaacg tcattcatat
     9961 ccagttctaa cgtactggta aaaattggtt catcgcccgg gttacgccac atgccctgcg
    10021 ctttggcata tttttcgacc aactcgacct gatcttcgct gcgcccgctt aaacgcatgt
    10081 aatcgagggt gacagcatcg attgggaaga agccacaggt ggcaccatat tctggcgaca
    10141 tattggcaat ggtggcgcga tccgccagcg gtagtgaatc cagcccatca ccataaaatt
    10201 cgacgaattt ccccaccacg ccatgtttgc gcagcatttg ggtaacggtg agaaccaggt
    10261 ctgtggcggt aataccttca cgtaattttc cggtgagttt aaagcccact acgtccggga
    10321 taagcatgga aaccggctgg cctaacattg cggcttcggc ttcgatccca ccaacgcccc
    10381 accccagcac gccaaggccg ttgatcatgg tggtgtgcga gtcagtacca acgagtgtat
    10441 ccggataagc aatccattca ccgtcctgca attcgctcca cactgctttg ccgagatatt
    10501 cgaggttaac ctgatggcaa atgcctgtgc ctggcggcac gacgctaaaa cgactgaacg
    10561 cctgctttcc ccatttcagg aacacataac gttcgtggtt gcgctccatt tccaggcgca
    10621 cgttttcttc aaatgcctca tcatcaccaa aacgatcgac ggtcaccgag tggtcaatga
    10681 ccaggtcgac cggtgagagc gggttaacct ttgcagtatc gccgccgagg cgtttaaccg
    10741 cttcgcgcat tgccgccaga tcaacaacgg caggtacgcc ggtaaagtcc tgcatcagca
    10801 cccttgccgg gcggtaggca atttcacggt cagcatgggc atttttcagc catcccgcca
    10861 gcgcgtagat atcctcttcg gtaaccgagt taccatcctg ccagcgcagc aggttttcga
    10921 gcaaaacttt gagtgacttg ggcagacggg tgatatcgcc tagtgatttg gcagcaagcg
    10981 gcaagctgta gtagtggtaa gttttatctt tggcctgcaa cgtgtcctta ctggcttctc
    11041 gtagggttga cgacatagct cctccttaat gacagggttg cgaagccctg attctcttca
    11101 gggtgaatat taaagataac acaaacatcg cgtaacgatt tgataacaac ccaaattgat
    11161 aaaagaggag aaaccttgag acaaagcaaa cagcggggtc agggatcagt gagtaatgat
    11221 ccagaggagt tcggcccaga agagcatcga cagagaaaat gcgcccatcc aggaccaata
    11281 tttcatattt gtgttgatca taggctacac ctttaacggt tattactttg tgttgagata
    11341 actcaacctg ttattgctgc ggtgtattaa aagcccttta attcattaaa aatgggtaat
    11401 aagaatgatg ataattattg tttctgctga tcatcttcag caaacagttc tataaaggct
    11461 tgctgctgcg atgttagttg ccagtcgtct ggaataacga cggaacattc ccgtttcgtt
    11521 tctgtttcat tgtcactatg ctgacataat ggtgacgact ccagttgctc ctgaccgacc
    11581 atagttatcc ccacactttc caaatcagca gtgcgacaac cacccagaaa agagcagaac
    11641 caagaaatac tgccagccag gctttgcgtt ttaattcagg gtcccggcgg ggttcctgat
    11701 ttccagaagg cattgctaac ctcatataat cgccatcact tatcagcaag acgttgaacg
    11761 attggtgtaa ttaatcatac gatgagacaa atcctaaaga aactttagtc aaaaatccag
    11821 cgaatttacg aatcgtctcc agtgaataat tcaatctttt gacctgaaat agatatttga
    11881 aaactcgagt ttgcgaggcg ggtaattaga cactacttta caggtaattt accctataaa
    11941 tatgagggat aaaaatattg tgcacggtat acaaataagc aacaaaagag aatatattcc
    12001 ggcaccttcg ctacataaaa ggtgccgaaa ataacgcaag aaattatttt tccggcagtt
    12061 ttatatcttt aaacatgacc tcaatttctt cattagagcg caatgcgaca gccgcatcaa
    12121 cgacatcacg cgttaaatgc ggtgcaaaac gctgaatgaa atcatacata taactgcgca
    12181 aaaaagtact acggcgaaaa ccaattttgg ttgtactgtg gctgaagata tcgtgagcat
    12241 caacgcgcac caggtcggga tcggcgaccg gatccaccgc catgctggca atgaccccta
    12301 cccccaaccc taaccggacg taagttttaa tgacgtcagc atccgttgcc gtgaagacga
    12361 tacgtggcgt taaccctgcg cggttaaagg cagtatccag ttctgagcgt ccggtaaagc
    12421 cgaaggtata tgtcaccaac ggatattgcg ccagttcttc aatggtaatg gcttttttgc
    12481 ctgccagcgg gtgatccgga gtgactacaa tagcccgatt ccagtggtag cacggtaaca
    12541 tcactaaatc ttcatacaga tgcagcgctt ctgtggcgat agcgaaatcg gcattgcctt
    12601 tagagacagc atcagcaatt tgtgtcggcg agccctggtg catatgcaaa gaaacgcgag
    12661 gataacgctc aataaagcct ttgatgacgt ttggtaacgc atagcgtgcc tgggtatgcg
    12721 tggtggcgat atacagcgag cctttatccg gccaggtgtg ctctccggcg accgatttta
    12781 tggcatcgac tttcgacagg acttcacgag cgatacgaat tatttcttgt cctgctggcg
    12841 ttacctgcgt caggtgcttg ccgcttcggg aaaaaatttg aatgcctagc tcgtcttcca
    12901 gcattctgac ttgtttactg atcccgggtt gtgatgtgta aagtccttcc gctgttgatg
    12961 agacattcag gttatgattg accacctcaa caatatagcg aagttgttgt aatttcatgt
    13021 taaaccatcc acttagacgc catcagaaca tcgttttttc gtcacgccag gcgggtgact
    13081 aatacgattt aataataaaa aaggggctaa ctataaccac tatatcattt atagccttag
    13141 tgtataggtg tgaagaaaaa ataatatgca ataaaaaagg gccgctttcg cgaccctttg
    13201 tttataaaaa cctgacaaaa ttaaaggtta ttttttccct tcaacccatt tgccatcaac
    13261 ataaaatgct gaccagccag tcgcctttcc gtctttttcc gaagagacat attgctgttt
    13321 ggttttacgg ctaaagcgaa ccatggtctt attaccttcc ggatcctgct gtggcgcatc
    13381 ggccagataa cgcagttttt ccggcagacg gtcgcggaag cgataaagct cttccaccag
    13441 tggcgcacgt gtttcacgcg atttcgggaa agtgttggca gccaggaaca caccggcagc
    13501 accgtcacgc agcacgaaat aggcatctga tttttcgcac ggcagctccg gtaatggcac
    13561 cggatcttcc ttcggtggtg ccacttcgcc gttacgtaaa atcttacgtg tgtttttaca
    13621 ctcttcgtta gtgcaggcca tgtatttacc gaaacgcccc attttcaggt gcatttcaga
    13681 gccacatttt tcacactcaa cgatcgggcc gtcataacct ttaatgcgga attcgccctc
    13741 ttcgatctcg taaccgtcgc aggttgggtt attaccacag acatgcaact tacgtttcgg
    13801 atcgatgaga tagctgtcca tcgccgtgcc gcatttcggg caacgacgtt ttgcgcgcag
    13861 cgcgttggtt tcagcgtctt cgccttccag cacgttcagc acttcgtttt ccggcaccag
    13921 gttaatggtg gttttgcaac gctctttcgg cggcagcgca tagccagaac agccaaggaa
    13981 taccccggtg ctcgctgtgc gaatccccat tttgcgacca caagtcgggc agtcaatgct
    14041 ggtcagaacc atctggttcg ggcgcatacc accctcttcc ggatcttttt cagctttatc
    14101 taactgctgg gtgaaatccg agaagaagtg atccagtaca gctttccact ctgcttcgtg
    14161 atttgccacc tggtcgaggc tgttttccat ctgcgcggtg aagtcgtagt tcattaactc
    14221 gcggaaattc tcttccaggc gatcggtgac gatttcgccc attttttccg catagaaacg
    14281 acgattttct actcgcacat agccacgatc ctgaatggtc gaaatgatcg acgcataggt
    14341 agacggacga ccgataccac gtttttccag ttctttaacc agcgatgctt cactgaaacg
    14401 ggctggcggc ttagtaaagt gctgggctgg tgtcagttca acgagcgtca gagcatcgcc
    14461 tttatcgact gccggtaaga tacgatcttc atcgccttta cgcagtgcag gcatcacttt
    14521 tgtccagcca tcaaagcgca aaatacgacc gcgtgctttc aggcggaaat cgcccgcacc
    14581 aacggtcagc gtcgtggagt catatttcgc tggggtcatc tggcaggcaa cgaactgacg
    14641 ccagattaac tggtacagtt tctgcgcatc tgcttccata tccttcagcg attccgccat
    14701 cacattgacg tcagaaggac gaatcgcttc gtgcgcttcc tgtgagtttt ctttgctggc
    14761 gtactgattc ggactttccg gcagatattt cttaccaaaa ttatcgctga tataaccgcg
    14821 aaccatattt accgcgtcct gactcaggtt agtggagtcg gtacgcatgt aagtgatata
    14881 gcctgcttca tacaaacgct gagccatcat catggttttt ttcacgccaa atccaagacg
    14941 ggtgctggca gcttgttgca acgtagaggt aataaaagga gcgccaggtt tactggttgt
    15001 cggtttgtct tcacgttcca gcacgctgta gcgcgctttt tccagcagac ttaccgcagc
    15061 ctgagtttgt tctttgttga ccgggcggaa cggtttgtcg ttctgatgag tcacctgcaa
    15121 cgccaacgca tcaccagatg gcgtggtcgt gctggcatcg acttcccaga actcttccgg
    15181 tacgaacgct ttaatttcac gctcacgctc gaccaccagg cggaccgcca ccgactgcac
    15241 acgaccggca gacagaccac gagcgatctt tttccacagc agcggcgaaa ccatatatcc
    15301 caccacgcgg tccataaagc gacgcgcctg ctgggcatta acacgatcaa tattcagctc
    15361 acctggtttg ttaaatgcct ggcggatcgc gtttttagta atttcgttaa acaccactcg
    15421 gctatagcgc gcatcatcac caccaatcac ttcccgcagg tgccatgcaa tggcttcccc
    15481 ttcgcggtca aggtcggttg cgagatagat gtggtcggct ttttcagcca gttgtttcag
    15541 ttcagagacg accttctctt taccaggcaa cacttcatag tgcgcctccc aattgtgcca
    15601 cgggtcaacc cccatacggt tgacgagagc gccacgttca tcctttttag gctttttagc
    15661 cgtcttggtg gaggtagagt cggcactctt tttggcagct gagccactgg tcggcaaatc
    15721 gcggatgtga ccgacgctgg atttcaccac gtagtcacta cccagatact tgttgatcgt
    15781 tttggctttt gccggggact caacgatgac aagagcttta cccatattca ccttacctaa
    15841 tttgattctt ccaggaatgc gtcgcacgtt gataaacctt ccactggcaa cgagttaccg
    15901 atatggactc gctctctgcg gatatcaacc ccttgtcgtg actctgttca cgaaaattca
    15961 ggtgactgag ttaacaggtc ttggcctaca ggcgctatag cacgacggaa ttttggtcga
    16021 atgtcaagca aatctattgc cagattgacg aaagcgtcac actgtacctg ataaaatccg
    16081 atacgcaact ttattagcat gcgaaatcca aagttgccag taaggcgctc ttgccaccag
    16141 cgtggtatat ttgtctgcaa gccatgcctg gcgtagacta atgtccttgt ttaaggagta
    16201 aataatgaac aaagagacac aacccatcga ccgcgagacg ctgttgaaag aggccaacaa
    16261 aatcattcgt gagcatgagg atacgctcgc agggattgaa gccaccggcg tcacgcagcg
    16321 taatggcgtg ctggtattta ccggcgacta ctttttagat gaacaaggat tgccaacagc
    16381 caaaagcacc gcggtgttta atatgttcaa acacctggcc catgtgcttt cagaaaaata
    16441 tcacctggtt gattaaagca aaacgcgagg tcttaccctc gcgtttgtct tttacatcaa
    16501 tggcttttgc ccccgctgcc accagcgtaa caacaatcga tcggcgctct ctgccgcgct
    16561 gccggtgaat cggtcaatga gtcgtttacg ctgcatatag cgtacattga ccacttcacg
    16621 gccttccatc aggctaagaa taacttcatc actggtgttg atttcatcaa ccaggccttt
    16681 ctctaccgcc tgttgtccgt accagtgttc acccgttgcc acctgatcaa tattcagaga
    16741 cggacgcata cgcttcacaa aatctttaaa taactgatgc gtttcgttca gctcttcgcg
    16801 gaatttctcc cgcccttctt cggtgttttc acccagcaag gtcagcgtgc gcttatactg
    16861 cccagcggtg tgcagttcga tatcaatatc tttgcttttc aggaagcggt taaagttggg
    16921 catttgcgcc accacgccaa tggaacccac aatagcaaac ggtgcggaaa caattttgtc
    16981 cgccacacag gccatcatgt aaccgccgct ggcagcgact ttgtctaccg taacagttaa
    17041 aggaatgttt ttatcacgca gacgctgcag ctgcgaagcc gccaacccgt aaccatgcac
    17101 catgccacca gggctttcca gacgtagcac aacctgatcc tgcggtttga atgctgcgag
    17161 taccgccgtt atctcttcac gtagcgagtt cacttcatgg gcgtccatgc tgcctttaaa
    17221 atccagcacc cagacgcggg gtttactgtc agttgccacc tcgcccagtt tggctttcgc
    17281 ttttgctgct ttcgcttctt gcttgtgctt tttcttctgc gctttatgcc actgtttttg
    17341 ctgatgagtg tccatcagcg ccgcggccag ttcttctttc atctccttat actgttcgct
    17401 gagattgttg acccgtaact cgccacgctg gcgtttatta cgttgagcaa cattgacaat
    17461 aatggcggca atcgccgcaa tcgctagcac aacggtaacg attttcgcca aaaacaaacc
    17521 atattcagac aacaattcca cgagtcccca ccttggttaa acaacgcttt ggctcacagt
    17581 ttacaacagg catgttaagc cgtctcgcag agatgttatt gcagcgaaag tatgacaaag
    17641 tatccatttt gcggtgttat cgccccaggc tgattgaaat gcccttctgt ttcaggcata
    17701 aagccccaaa gtcataaagt acactggcag cgcgacagac aacgtcgttg aggagtagcc
    17761 atgcattacc agccaaaaca agatttactc aatgatcgca ttatcctggt gacgggagcc
    17821 agcgatggta ttggccgtga agccgcgatg acgtatgcac gctatggtgc gacagtgatt
    17881 ctgttgggcc gtaatgaaga aaaattacgt caggtagcca gccacataaa cgaagaaact
    17941 ggacgtcagc cacagtggtt tattctcgat ttgctgacct gcacgtccga agattgccaa
    18001 caactggcac agcgcattgc cgttaattat ccgcgtctgg atggtgtttt gcataatgcc
    18061 ggattgctcg gcgatgtttg ccctatgagc gaacaaaatc cgcaggtctg gcaggacgtc
    18121 atgcaggtca acgttaatgc cacctttatg ctcacccagg cactgcttcc tttattactc
    18181 aaatcggacg ccggttcact ggtctttact tcatcaaacg ttggacgtca gggacgagcc
    18241 aactggggcg catatgcagc gtcgaaattt gccaccgaag ggatgatgca ggtactggcc
    18301 gatgaatatc agcagcgcct gcgcgtcaac tgcattaacc caggcggtac gcgcaccgca
    18361 atgcgtgcca gcgccttccc aaccgaagat ccacagaaac ttaaaacacc cgctgatatc
    18421 atgccgctct acctctggct gatgggcgat gacagccgcc gtaaaaccgg catgaccttt
    18481 gacgcccaac cgggccgtaa accaggaatt tcccaatgag tgatgaacgc taccaacagc
    18541 gtcagcagcg agtgaaagaa aaagtcgatg ctcgtgtggc ccaggcccag gatgagcgcg
    18601 gtattatcat cgtctttacc ggcaatggaa aaggcaaaac caccgcggca tttggtacgg
    18661 caactcgtgc agtaggccat ggcaaaaaag tgggtatcgt gcagtttatt aaaggcacct
    18721 ggcctaatgg cgagcgcaat ctactggagc cacatggcgt tgagtttcag gtgatggcaa
    18781 cgggctttac ctgggataca caaaatcgcg aatctgatac cgcggcctgc cgtgaagtct
    18841 ggcaacatgc caaacgaatg ctggccgatc cttcactgga tatggttttg ctcgatgagc
    18901 tgacttatat ggtggcgtat gactatttgc cactggaaga agtggtgcag gcgttaaatg
    18961 aacgtccaca tcaacagacg gtgattatca cgggtcgagg ctgtcatcgg gatattcttg
    19021 aactggcgga tacggtgagt gaattacgcc ccgtgaaaca tgcgtttgat gccggtgtaa
    19081 aagcgcagat agggattgac tactaagtga aagcacctca aaagaagcct cttgaggtgc
    19141 tttaatcatg attatattca agcggcaata gcgaaattca gaatacgtgt aatctcattc
    19201 aaatgtccct gatatgcttc atgattctca gcgtcaacat cttcaataaa taatatcagt
    19261 tcagtaatta acgcattggc atcttcataa ctgattctgc ttaactctgg cgaagtttca
    19321 gtaccgtagt taatcgtcag attgttcatc accgtgagga taaggataaa atatttatga
    19381 caaacattac atgtggtttt attacccaaa gtattataca gcatatcaag taatgccagt
    19441 attctacggt tttgcacatc gttcataaaa agcctcctat aacattattc cgctggcaat
    19501 aagtaatttt gccagcccct cagacgcctt taacccacta atgacctgtg cgcgtaatat
    19561 ttcatttaat cttttttgtt cagctgccgt gcgtgtttta ttaacgtata agcttttcag
    19621 ataatcaccc ttatctataa aggcattcaa agcctcaatc gcaggcatgg caggtgccga
    19681 tgcctgtagt ctggtttcat tcacattgat tactgaactt agaaactcct tcctgttttt
    19741 cagagtgtta aactct
//
LOCUS       DAPHCV010000093        19295 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.093, whole genome shotgun sequence.
ACCESSION   DAPHCV010000093 DAPHCV010000000
VERSION     DAPHCV010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.093"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            376..525
                     /locus_tag="RH033_003415"
     CDS             376..525
                     /locus_tag="RH033_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
                     /protein_id="HDV2992555.1"
                     /translation="MEKVISHTRYKLTSAELEAFNSVVDHWLAELTMNKLYDRVPASV
                     WKYVI"
     gene            1340..1909
                     /locus_tag="RH033_003416"
     CDS             1340..1909
                     /locus_tag="RH033_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HDV2992556.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLERDDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            2169..2570
                     /locus_tag="RH033_003417"
     CDS             2169..2570
                     /locus_tag="RH033_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992557.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGERYCTHCQTGGFSIPRTTQEEICEF"
     gene            2558..2968
                     /locus_tag="RH033_003418"
     CDS             2558..2968
                     /locus_tag="RH033_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HDV2992558.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSREGGLQEGNWKDRN"
     gene            3252..4871
                     /locus_tag="RH033_003419"
                     /pseudo
     CDS             3252..4871
                     /locus_tag="RH033_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            4951..5243
                     /locus_tag="RH033_003420"
                     /pseudo
     CDS             4951..5243
                     /locus_tag="RH033_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(5283..6317)
                     /locus_tag="RH033_003421"
     CDS             complement(5283..6317)
                     /locus_tag="RH033_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase"
                     /protein_id="HDV2992559.1"
                     /translation="MKDYLQTVTGPVAREDMGLTLPHEHLFNDLSSVVDAPCYPFSQR
                     LVDKKVTAEIQWALKHDPYCCADNMDRKPIEDVIFEINNFISLGGRTIVDATGSESIG
                     RDAQALREVALKTGLNIVASSGPYLEKFESQRIHKTVDELATTIDKELNQGIGDTDIR
                     AGMIGEIGVSPTFTEAEHNSLRAASLAQINNPHVAMNIHMPGWLRRGDEVLDIVLGEM
                     GVSPNKVSLAHSDPSGKDVAYQRKMLDKGVWLEFDMIGLDITFPKEGIAPGVQETADA
                     VAHLIELGYADQLVLSHDVFLKQMWAKNGGNGWGFVPDVFLAYLAERGVDKTILKKLC
                     IDNPGRLLTA"
     gene            complement(6320..7285)
                     /locus_tag="RH033_003422"
                     /pseudo
     CDS             complement(6320..7285)
                     /locus_tag="RH033_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462450.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            complement(7342..8100)
                     /locus_tag="RH033_003423"
     CDS             complement(7342..8100)
                     /locus_tag="RH033_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HDV2992560.1"
                     /translation="MNNTKKSLKVLFIGESWHIHMIHSKGYDSFTSSKYEEGATWLLQ
                     CLKNSQVDVTYMPAHTVQIAFPEDVAQLEQYDAIVISDIGSNTFLLQNDTFYQLRIKP
                     NALELIKEYVNNGGGLLMIGGYLSFMGIEAKANYKNTVLADVLPVTMLDGDDRVEKPE
                     GVIAQPSQPEHPVIKGFSEYPFFLGYNRAIAKENAEVVLTINNAPLLVFGNYHNGKIA
                     CFMSDCSPHWGTQQFMSWPFYTALWVNILTHIAR"
     gene            complement(8114..9328)
                     /locus_tag="RH033_003424"
     CDS             complement(8114..9328)
                     /locus_tag="RH033_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462448.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDV2992561.1"
                     /translation="MKFERHHEILKRLSKFGSVKVSDLSNSLNVTKETIRSDLNELAR
                     LGYLTRCHGGAFIVLDSLDTIAKNEIAYALENYDTAQGIKKGHSTMKSQVCVIGSFNV
                     DIISYLPRLPTIGESLLASNFIFSPGGKGCNQALAASFADTDVYFITKVGTDHFSDYA
                     INFMNSSKIYKSIIYQTKETQTGTATILVNEGTGDNVIAIYPGANMTMSSDEITIQKE
                     AIINSDVILLQLETNYTALQQAITLAQKNSIPVIINPAPYNDIVNELIQDVDYITPNE
                     TEAGLLSGIDVHDLESAKRAAEAIHNKGVKNTVITLGSKGSLAFDGKKFIHSPAFPAV
                     VKNTAGAGDAFNGSLASGLAKGKSLESALCYASAFASLAVETSNASDMPEHESVIHRI
                     QSIHYQQTIFTH"
     gene            complement(9524..9862)
                     /locus_tag="RH033_003425"
                     /pseudo
     CDS             complement(9524..9862)
                     /locus_tag="RH033_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            11379..11924
                     /gene="cobU"
                     /locus_tag="RH033_003426"
     CDS             11379..11924
                     /gene="cobU"
                     /locus_tag="RH033_003426"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HDV2992562.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            11921..12664
                     /gene="cobS"
                     /locus_tag="RH033_003427"
     CDS             11921..12664
                     /gene="cobS"
                     /locus_tag="RH033_003427"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HDV2992563.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            12676..13755
                     /gene="cobT"
                     /locus_tag="RH033_003428"
     CDS             12676..13755
                     /gene="cobT"
                     /locus_tag="RH033_003428"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HDV2992564.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVVDVGIDSAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            13817..14752
                     /gene="ldtA"
                     /locus_tag="RH033_003429"
     CDS             13817..14752
                     /gene="ldtA"
                     /locus_tag="RH033_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDV2992565.1"
                     /translation="MMRRVNIFCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(14808..14883)
                     /locus_tag="RH033_003430"
     tRNA            complement(14808..14883)
                     /locus_tag="RH033_003430"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14848..14850),aa:Asn,seq:gtt)
     gene            15210..16127
                     /gene="nac"
                     /locus_tag="RH033_003431"
     CDS             15210..16127
                     /gene="nac"
                     /locus_tag="RH033_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HDV2992566.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCELAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            16229..17179
                     /gene="cbl"
                     /locus_tag="RH033_003432"
     CDS             16229..17179
                     /gene="cbl"
                     /locus_tag="RH033_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HDV2992567.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKSFRELFPEVRLELIQGTPQEIATLLHNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGIVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(17217..17292)
                     /locus_tag="RH033_003433"
     tRNA            complement(17217..17292)
                     /locus_tag="RH033_003433"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17257..17259),aa:Asn,seq:gtt)
     gene            17297..18940
                     /gene="yeeO"
                     /locus_tag="RH033_003434"
     CDS             17297..18940
                     /gene="yeeO"
                     /locus_tag="RH033_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HDV2992568.1"
                     /translation="MLRHILTAKNLLSNPIFNFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPAVSE"
     gene            19041..19116
                     /locus_tag="RH033_003435"
     tRNA            19041..19116
                     /locus_tag="RH033_003435"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19074..19076,aa:Asn,seq:gtt)
BASE COUNT         4922 a         4287 c         4920 g         5166 t
ORIGIN      
        1 gagatcaaat acatgagttc atatatgtga aataaacacg gtgagtgtga tcagtgggca
       61 tgatgagaac gcagtagcaa cagactgcgc tatccctgat agctggcgta taacccgcgt
      121 atataaaacg tatctgcaat gtacgtgaac agagtgcatt aagcatccgt ctgagcggta
      181 aacatatccg acctggtgag tgacagtctg agagaccggt taagtggtgg ctcgggagta
      241 atgagtttgg ggtgggtacc agaatgccag tctggcggta tgctgacagt atcacgttaa
      301 ttgcagcata aagtgatgag aggctaatgg aagttaaaac caaagcagtt ccgtcgttgt
      361 tcatcccggg agacgctgga aaaagtgatt tcccacacgc gttataaact tacctctgcg
      421 gaactggaag ccttcaactc tgtggttgat cactggctgg cagaactgac aatgaacaag
      481 ctttacgatc gtgttcccgc ttcagtctgg aaatatgtca tctgagcgtt cggtaatcac
      541 aactgcatcg caccaggtgt tatccgcttt gtgattctta ttggctcttc tcaggttatt
      601 aaaagctgaa tatgttcctg ttgtcgggaa ccaggataat accgggaacg ttaagcggta
      661 tagcgacaac aaagaattcg aaatcaaaag ggaatggtgc cggaggcgtg ggcggtaatg
      721 ggctgatttt caggtattac cggcttacag cgtgtggttg atattgaggg gctgtgagtt
      781 tcgcgtattt gtcagaaacc cagacgagtt ttcctgtatg ggacgaaata aggcagcggc
      841 aatacctgga aaaatttaat atggtatgaa ggagaacctg tcggttacgc ttaatatacc
      901 cggtacactg tcagctcagg ggatacatga accgttatct caccagctac acagtatata
      961 cacactgact gacgctgaca gtagtcagca ggaccacaat aatcagtgcc agcagtatgg
     1021 taacgatacg gtctgtattc cggtaatagc cgaaggggtc gacagaacca caatacgggc
     1081 aacatgtatt atgagtaatc gcgtttccac agtccctgca cttctttttg cgtatcacca
     1141 taactccctc ctcacagcct tacccgtaac cggtttttta cattaaaaaa acaacgctga
     1201 aaattcaacc gtcagtccgg agacgatagt tggggtatca cagagtgcct gagacagtgt
     1261 cctgccggag gtcacttcag ggactctgcg cattttttac gacgtggcta ttccgtatga
     1321 accatacgga gatttaacca tgacctacaa atacaacccc ttctggcagc aacgtattcg
     1381 tgagacggtg cggcacgcac tgaatgttca tccccgcctg acggcattgc gggttgacct
     1441 gcgtttcccg gatgtaccgg cagcaacgga cgcagctgtg atatcccgct tcatcaatgc
     1501 cctgaaagcc cgaatcgacg cttaccagaa acgtaagcat cgggaaggta aacgcgtgca
     1561 tcccaccacc ctgcattacg tctgggcccg ggagtttggg gagtgcaaag gtaaaaaaca
     1621 ctatcacctg atgctgctgg ttaaccggga tacctggtgt cgtgccggtg attaccgtgc
     1681 tccgggatca ctggccggga tgattaaaca ggcgtggtgc agtgccctgg gagtggatgt
     1741 cgggtgccat gccacgctgg tgcattttcc ggcctggccg gcggtgtggc tggaacgcga
     1801 tgatgacact ggctttcagc aggtgctgga acgtgctgac tatctggcga aggaacatac
     1861 caaagctcac tgcaccggtg agcgcaattt tggctgtagc cggagttgag cacgactggc
     1921 gcgccaatat ccggcaaacc aaaagtatgg gttgttgtct ggcggcaacg atgtatccac
     1981 tcgcccactg actgtttttt acgtcagtgt tccattgtgt gacaaccaca gtgctgtaac
     2041 agcaaccagc ctgctttacc caaactccag tccagatttt aactgaaccg ccattcacac
     2101 cctgaccacg ctgcccgtac cgggacggct gttgcctgcg tgtcgtctgg cggctgagga
     2161 atatgactat gtacgcaaaa tcctttatcg ctcttgatgg caacggacgt ctgacgggcg
     2221 cccgtactgc acaggccgca ccttatgcta actacacctg ccacttgtgt ggcagtgcac
     2281 tcagatacca tccgcaatac gacactgaac ttccctggtt tgaacacact gacgacaggc
     2341 tgacagagca cggtcaacag tgcccttatg tcaggccgga gcgcagagaa atacagttga
     2401 ttaaacgtct gcagcaattc gtaccggatg ccttacccgt ggtgcgtaaa gccagctggc
     2461 actgcagaca atgtcaccac gattattatg gggagcggta ctgcacacac tgccagaccg
     2521 gaggattcag tattccccgg acaactcagg aggaaatatg cgaattctga actgttatat
     2581 ggcgaatgac agcaaaggcc attttgttac ggcgaaagaa gcagcgaagc acaaccgcca
     2641 ggacgttttg tgctgtgtgt cctgtggatg cccgttaaca cttcagcggg gcaatgacgg
     2701 acaaccaccg tggtttgaac atgaccagat gactgtcgct gagaaaatcc tgctgcgatg
     2761 cacctggctt gacccggcag agaaagaggc ccgtcgtttg catctgcagg gtatgacggt
     2821 tccggattat acggtgaagg tgagaaagtg gttttgtgtg atgtgtgacg aagattatga
     2881 aggagaaaag tgctgcccgc gttgtggtac cggggtatac agcagggagg gggggctgca
     2941 ggaaggcaac tggaaggata ggaactgagc ggttcagtcg cggaaatcag ttgcagagct
     3001 gtaatgatat ctccgggagt gctgcgttgg taggggcatc ccgggataga gaaattttag
     3061 cactttgccc tgaatgattg tagacgttcc gagtactcgt accggtctga gttattacag
     3121 ttagatattc actttttctc ccgcatactt cacatctgtc aacgacggat gaaaagtggt
     3181 tcttccgcaa cctgggtgga agaaaatgga tgaaactttc tggcgaacaa ggatctaagc
     3241 tgttttaagt tatgggcaac gcaatgcact ctcttaagac acttctacag ttaccttgcg
     3301 gatggcgatg cagtcgacaa attattagct ctgacggtat aaccctccat ctccacggaa
     3361 aacgcaaaac agcacaatgt cctgaatgct ttaagcgtag cgactctgtt catagttgtc
     3421 gtcggcgccg gatacagcat ctaccctgct cagggcagac gctatggctt gtattttccg
     3481 tccgccactg gtactgccgt aacccggctt gttcacgtaa aatttttgcc gagtcgcttg
     3541 ctcccttcgc cggttcacac cagcagtctt cacaggcgtt acaaaattta caacgtcaac
     3601 tgggattaat agctggaggt gaggctggaa aacgggctgc aacggcagcg ggtctccgtt
     3661 gcagtgctga tactcttctt cgcagggtta tcaatacccc ggagacgaaa cagtcaggcg
     3721 cgcctcatgt cggtattgat gagtgggcgt ggcatcgggg ccactgctgc ggtatgttaa
     3781 tagtcaatct tgatactcac cgtcccctcg tcctgcttcc cggccgtgat cagcgtacgc
     3841 tggcgacctg gttcagaaaa tatccggaaa tacaggttgt ctcgcgtgat cgcagtggag
     3901 tctatgcgac agcagcacgt gaaggtgcac ctcaggccag acaggtggcc gatcgatggc
     3961 acctgctaaa aaatattggt gatgagcctg aacgaatgat gtacagacat atgcctctga
     4021 tacgtcttgt tgtcagagag ttatcactga agaaatcacc tgagccagaa atatctgtgc
     4081 ctgtagcatc gctccgtcgt ctggaacgcc ttaaacagca catccgcaaa aaacggcatc
     4141 agcgttggac agaggttatg gccctgcata acaagggatg tagtttcagg gaaatatccc
     4201 gtattacagg cctgtcgcga gtgacagtca gtcgctgggt gggttcagga acattccctg
     4261 aaatgtcaac taggcctcca aagcgagggc ttctggaccc atggagggag tggttaaaag
     4321 agcaacgaga atgtggtaat tataactccg gccggatatg gcgggaaatg gtggccaggg
     4381 gggttacagg cagtgaaacc atcgtcaggg atgctgttgc caaatggcgt aaaggctgga
     4441 tcccaccggt tactactgcc gcaagacttc cttcagtgtc ccgggtaagc cgctggttga
     4501 tgccctggag aataatcagg ggtgaagaaa attatgcttt ccgatttatt agtctgatgt
     4561 gtgaaaaaga accggagttg aaaatagcgc agcaactggt actcgagttc tatcgtattc
     4621 tgaaaaccta aaataaatca cagctaagca gctggttcac tcgggtccac gaaagcggct
     4681 cagcagaact tcggtgcgta gctgcgggga tggaagctga tgctgcggct atatgtgagg
     4741 caatcagcag tcgctggagt actggtgttg tcgaaggtca tgtaaatcgc ctgaaggtgc
     4801 tgatacgcca gatgtatggt cgagccggac ttgaactact taggcggagg gtaatgagtc
     4861 cactggcatg aaaatcttca cccaggttgc ggaagagcca cttttgcact gttcgtgaca
     4921 gtatggcctt tatgtactca ctttgttacc ggtagctgga tcatcactac cgtgctgcaa
     4981 atggtggtga acagcatcaa ttgtggaatg aatgttgccg aagcgaccaa tgcgcctcgt
     5041 ttccactatc agtggttgcc ggacgagctg cgtgttgaaa agggtttagt ccggatacgc
     5101 tcaagctgct ggatgcaaaa ggtcagaaag tggcgctgaa tgaggcgatg ggcagcacgc
     5161 aaagcattat ggttgggtcg gatggtgagt tgtacggcgc atccgactcg cgcttggtgg
     5221 atgatttaac ggcggggtat taattgacat cccccggcgc aatgtgccgg gggataatgg
     5281 ttttatgcgg tcaataatcg tccgggatta tcgatacaga gttttttgag gatcgtttta
     5341 tcgacgccgc gctccgccag ataggccaga aaaacatctg gaacaaatcc ccagccatta
     5401 ccgccatttt tagcccacat ttgtttaagg aagacatcgt ggctgagaac aagctgatcg
     5461 gcgtatccca actcaatgag atgagcgacg gcatcggcag tctcctgcac ccctggcgct
     5521 attccctctt tcgggaaggt aatgtccagg ccaatcatgt cgaattccag ccagacacct
     5581 ttatcaagca ttttccgctg atacgccacg tcttttcctg acggatccga gtgtgcgaga
     5641 gagactttat ttggcgagac gcccatttca cctaacacaa tgtcgagtac ttcatcaccg
     5701 cgacgaagcc agcccggcat gtgaatattc atcgccacat gaggattgtt aatctgtgcc
     5761 agcgaggcag cccgcaagct gttatgctcg gcttcggtaa atgtcggtga aacacctatt
     5821 tcaccgatca ttccggcacg aatatccgta tcgccaatcc cttgattcaa ttctttatcg
     5881 atcgttgtcg ccagttcatc aaccgtttta tgaattctct ggctttcaaa tttttccagg
     5941 tagggaccgg aggaagcaac aatattcaga cctgttttta atgcgacttc tcgtaatgcc
     6001 tgcgcatctc gtccgataga ctcagaccct gtggcatcaa caatggtgcg accaccgagc
     6061 gagataaagt tattaatttc gaagatcaca tcttcaatag gtttgcggtc catattatca
     6121 gcgcagcaat aaggatcgtg ttttaatgcc cactggattt ctgccgtgac ttttttatca
     6181 acaagccgtt gtgaaaaagg atagcaagga gcatccacaa cagaggaaag atcgttgaac
     6241 agatgttcat gaggaagtgt tagccccata tcttcacggg cgacggggcc cgtcacagtt
     6301 tgtagataat ctttcatgat tatacctcca ttttcttcat tgttttcgag gttctgtatt
     6361 ccggaatagt ggtaacaatt gcaccgacca gtgcaaacag tgttccgatg atcgtgacca
     6421 gataaacgct attgcccagc gatggaatta atacgtcaat caatactgat cccagtaact
     6481 ggcccgccgt tgaagcaacc cccagcatca gtaaccccag acctctgacc aaaatggcca
     6541 ttaaaccgat agacattagt ccaagcgggc cgccaagata catccaccag acatctggta
     6601 attgaacagt gacgtgccct aatgcgacac gaatagctag cgctgtgcct aaaacacaaa
     6661 atccaacgat gaaattctag gtgattgaaa ccagcatgga tcccgtagct tcagctactt
     6721 ttgcatttcc agcgggctgc catcctgcaa gcaaaccagc taagaacgga agaatggcaa
     6781 gtaagatgaa agaggtagaa tgccactgag gtgacacaac aaaaatcgta gcaataaccg
     6841 caaataatgc accgatgatg cgataagggg taaagtattt tttctcgtcg acaccgatac
     6901 caaagcgatc gcaaagtaaa ccagacagca atagcgcgga gatcagtgcg gtttgaaacg
     6961 ttgcgacccc gagtgcgctg gctgatgcgc cttcagaaaa taccaccatc gctccacaga
     7021 gaccggcaaa ccagttccag agaggaactt ttcggttttt aatcagagaa ggaattgccg
     7081 caaattgttg ccgggtttct tttcgcgcca taataataaa aaacatcacg actaaaccac
     7141 tggcaaagga gataactgcg catgcattac catcctgtaa ccagtgacct aattgaccat
     7201 tcaccgctga ttgcataggg gagagcatac ccgcaagaat cgtggcaagc atccataagg
     7261 gagttgagta cttattcttt ttcatacatc aatccttcta ttatagtaaa tggagtaact
     7321 ggagttaccc cattattatt attatcttgc aatatgagtc agtatattta cccacaacgc
     7381 ggtatagaac ggccaggaca taaactgctg tgtaccccag tgcggtgaac agtcactcat
     7441 aaagcaggct attttcccgt tgtgatagtt accaaagacc agtaatggag cgttgtttat
     7501 tgttaatacc acctcagcat tttctttagc gatcgcccgg ttataaccta ggaaaaaagg
     7561 atattcagag aaccctttaa taactggatg ttcaggttgt gatggctgtg caataacacc
     7621 ctctggtttt tcgacacgat cgtcaccatc taacatcgta accgggagca catcagccag
     7681 aactgtattt ttataattgg cttttgcttc aattcccatg aatgagagat agccgccaat
     7741 catcaataac ccaccaccgt tattcacata ttctttaatc agttcgagag cgtttggttt
     7801 tatgcgaagc tgataaaaag tatcattttg tagcaggaaa gtattactac caatatcact
     7861 aataacgatg gcatcatatt gttctaactg tgccacatct tcagggaagg caatttgtac
     7921 cgtatgtgct ggcatatagg tgacatctac ctgactattt ttcagacact ggagtaacca
     7981 ggtcgcacct tcttcatatt tacttgaggt aaaactgtca tatccttttg aatgaatcat
     8041 atgaatatgc caggattccc caataaagag cacttttagt gattttttgg tgttattcat
     8101 tctctgtccc ttcttaatgc gtaaaaatag tttgctgata atgtatactc tgaattcgat
     8161 gtatcacaga ttcatgttca ggcatgtcgg aagcattgct tgtctccacc gctagggatg
     8221 caaaggcgct ggcataacac agtgctgatt ccagtgattt tcctttcgca agtcctgatg
     8281 ccagagagcc gttaaacgca tccccggctc ctgccgtatt tttaacgacc gctggaaacg
     8341 ctggcgaatg aatgaatttt ttgccatcaa atgcgagcga gcccttgcta ccgagcgtga
     8401 taaccgtgtt ttttacccct ttattgtgga tagcttctgc agctcgttta gctgattcaa
     8461 gatcgtgtac gtctatgccg gacagtaaac cagcttctgt ttcattgggg gtgatataat
     8521 caacatcctg aataagttcg ttgacgatat cattataggg tgccgggtta ataatgaccg
     8581 ggatactgtt tttttgcgcc aatgtgatcg cttgttgcag agcggtataa tttgtttcga
     8641 gctgcagaag tatgacatca gaattaataa tcgcttcttt ctgaatagtt atttcatcgg
     8701 acgacattgt catgttggca ccgggataga tcgcaataac attatcgcca gtaccttcat
     8761 taacaaggat cgtggcagtt ccggtttgcg tttctttcgt ttgataaatt atacttttat
     8821 aaatcttaga cgaattcata aagttgatcg cataatcact aaagtgatct gtacctactt
     8881 ttgttatgaa ataaacatca gtatcggcaa agcttgctgc cagcgcctgg ttacagcctt
     8941 ttccccccgg tgaaaatata aaattacttg ccagaagcga ttctccgata gtgggaagcc
     9001 tcggcaaata actgatgata tcaacattaa acgaaccaat aacgcatact tgacttttca
     9061 tagttgagtg gccttttttt attccctggg ctgtatcata attttcaagc gcataagcga
     9121 tttcattttt cgcaatagta tcaagcgagt ctaggacgat aaaagcaccg ccatgacatc
     9181 gagtcagata gccaagcctg gcaagttcat taagatctga acgaatagtt tctttcgtta
     9241 cattgaggct attggacaga tcggatactt ttacagaacc gaatttactg agtcgtttca
     9301 atatttcatg atggcgttca aatttcatgc ttcacctcac ttcaattacg ttaatatcta
     9361 tcgatgtata ttgatgtgat aatcaccata aagtggcggg gaaaagcaaa aaaaagcaaa
     9421 aatagcctga gaaaaggtaa gcgttaatgg agaatcatat tgatgcttat ttattggtga
     9481 gaactacgtt ccatggcagg agttcatcaa tacggttgga aggttattcc ggcagtacgc
     9541 tcaggatatg gcgcagatac gcttccggat caatgtcgtt cagaaggcaa gtgccgataa
     9601 gcccatacaa cagtgcactg cgctcgccgc cgaagaacat aaagttttta tttccgagac
     9661 agatggcaca aagggctttt tccgcggcat tatgtctgcc tccgcctgac cgtcaacact
     9721 gtaataacag agtgcgcccc actgattcag tacacagctg aaccattctc ccagtctgga
     9781 tttttggata gcgtggcatt tttctccacc atgcatgcag tgagtccagt aacagtttgc
     9841 tcttcttctg cctgaccaca agatgtgctg gactggtaaa cgatggggat acctgcttac
     9901 gcgagagcaa taagtggctg gccacaattc tcggtacaaa actctgctga atagcagcga
     9961 ttttcccagc gtggtgcgtc gtttgcattt tactggttct aagttaacaa ttatctgttc
    10021 actaatgact actctaaaaa cttgtatcag gactatcact tatttaagtg atattggttg
    10081 tctggagatt cagggggcca gtctataaac tttttacaaa aggatgaatg cagaaggtta
    10141 ctctaatggc cagagtgatg acaatgatgg tcagacgttg acctgcagaa actgcatttt
    10201 aattggtcta taaagtctgc aagaggaatc agggtcagat cagtgagtct tatcgcggcc
    10261 cgaagctatg actgaaagaa ggctggatac agaattgcaa caatttggca atgtcagaac
    10321 acagaagctt tgcaaacagg tgaaaggtta tgtctagcaa aaggagtata tcagaataca
    10381 tctactgatg tacaaaacac aacaatagct tcatgatata taaatacgtg atgacgagtg
    10441 gtactccgca tggttattta acttctcagc accactaatg atcctgagca acttcgtatt
    10501 tgggccattg ccatggagta gaaagctgtg tctgagaatg cagaatttga gaaaagaatt
    10561 cgctttcttg gagagcttct gaaaatggca cgctaacaac gttttgttaa aaaatgtgaa
    10621 tcacttgccg gggctgcatt ttctattcga ggagaatgtt gacgtagata ttacagaaat
    10681 ctcagcgcat caggataaac tgctacctta ggtcaaccac caaaagaaaa tacaatacgt
    10741 ccagtttgta tgcctttaat aacacatctt ttaaatgttg aagaattatc ccgcctgctg
    10801 aagaacgttg ctctgagtgt gataaacctt tttattcttg atgatgtcag cgagaagatg
    10861 gaatatatcc ccaactgggt cgtggttacc cggttaaaca tctggtggcg ctaataaatc
    10921 tggcaagtca cattctgttg aatgctgtga cagtgcctta ccgagagagc gaaattgtgg
    10981 tgttctagtg catagtcgcc accattccgg ataattctat tgcgttgttt tacaagttct
    11041 tttacagcgc agatctgtcg ctgacgttga atcaatagcc tggatttcag aacacaatgc
    11101 ccgtttgctg gttggtccgg cagtagcatt aattcattat ctgaatgaaa tgtgtcaatc
    11161 cacagaaacc gctgtcaatg acacattttg tcggacgtta acacgagaaa gtctggctat
    11221 tccgtaatgc catcttattt cgtaatggat tgagaatgct ggatttttaa ttatattacg
    11281 cgcagtatta atcgcgtaaa tgttttgcgg tcagcaacgg tgggggatcc attgttctgt
    11341 tcaatgaata ccatcataca tttgaatcag gagtcggtat gatgattttg gtgacggggg
    11401 gcgcacggag cgggaagagt cgccacgcag aggcgcttat tggggactct tcacaggttc
    11461 tgtatatcgc tacctcgcaa atccttgatg atgagatggc tgcacggata gaacatcatc
    11521 ggcaaggccg cccggagcac tggcgcacag tggagcgctg gcaacatctt gatgaattaa
    11581 ttcatgcaga cattaacccg aatgaggttg tgttgctgga atgcgttacc acaatggtga
    11641 ctaatctgtt gtttgattat ggcggcgata aagaccctga tgaatgggat tatcaggcga
    11701 tggaacaggc gattaatgct gagattcagt cgttgattgc tgcctgccaa cgttgccccg
    11761 caaaggttgt attagtgact aacgaagtgg gaatggggat tgtgccggag agtcgtctgg
    11821 cacgacattt tcgtgatatt gccgggcggg taaatcagca gttggccgct gcggcaaatg
    11881 aagtatggct ggtggtttcg ggtattggag taaaaatcaa atgagtaaat tattttgggc
    11941 gatgctctca tttattacgc gtctgcctgt accgcgtcgc tggtcccagg gactggattt
    12001 cgagcattat tctcgcggta ttattacttt tcctttaatt ggattattgc ttggcgcgat
    12061 tagcgggctg gtcttcatgg tgctgcaggc atggtgtggc gtaccactgg cggcactgtt
    12121 tagcgtactt gtgctggcgc tgatgaccgg gggattccac ctggacggcc tcgccgatac
    12181 ctgtgatggc gtattttctg cacgtagccg cgatcgcatg ttggaaatca tgcgtgatag
    12241 ccgtttaggc acccatggcg gtctggcatt gatttttgtt gtactggcaa agattctggt
    12301 attaagcgag ttggctctgc gtggcgaacc gatccttgca tcgctggcgg cagcatgtgc
    12361 ggtcagccgc gggactgccg cattattgat gtatcgtcat cgttatgcgc gggaagaagg
    12421 gcttggcaat gtgttcattg gcaaaattga tgggcgacaa acctgcgtca cactcggctt
    12481 agccgctatt tttgctgccg tattattgcc gggtatgcat ggtgtggctg ctatggtggt
    12541 gacgatggtg gcgattttca tcctcggtca gcttctcaaa cgtacgctgg gcgggcaaac
    12601 cggcgatacg ctgggagctg ctatagagct tggtgaactg gtcttcctgc tggcactgct
    12661 ataaagagac gtgacatgca aacacttgcc gatttactga atacgatccc tgctatcgat
    12721 cctgccgcta tgtcgcgtgc acaacggcat attgacgggt tactcaaacc tgttggtagc
    12781 ctgggaaggc tggaggcgct tgccatacaa ctggcgggaa tgccggggtt gaatggcata
    12841 ccgcatgtgg gcaaaaaagc ggtactggtt atgtgtgccg atcacggcgt ctgggaggaa
    12901 ggggtcgcta tttccccaaa agaagtgaca gccattcagg ctgaaaatat gacccgcgga
    12961 acaaccggcg tgtgtgtgct ggcagcacaa gcgggcgcta acgtccacgt agttgatgtt
    13021 ggtattgata gtgctgagcc tatccccggg cttatcaaca tgcgtgtcgc acgaggtagc
    13081 ggcaatattg cttcagctcc ggcaatgagt cgccgtcagg ctgaaaagtt gcttttggac
    13141 gtcatatgtt atacgcggga gctggcaaaa aacggtgtca cgctgtttgg tgtaggtgaa
    13201 ctggggatgg caaacacgac accggcagcg gcaatagtca gcacaatcac tggccgggat
    13261 cctgaagaag tggttgggat tggcgcaaac ctgccgacag ataaactggc taataaaatt
    13321 gatgttgtgc gccgggcgat tacgttgaat caaccaaatc cgcaggatgg cgttgatgtc
    13381 ctggcaaaag tgggtggatt tgatttggtc ggaatggctg gcgtgatgtt aggcgctgct
    13441 tcctgcggtt tacccgtgtt gctggatgga tttctttctt atgctgccgc gctcgcagcc
    13501 tgccagatgt ctcctgcaat caaaccgtat ctcattcctt ctcacctgtc ggcagaaaaa
    13561 ggggcgcgta ttgcgctgtc gcatttgggg ctggagcctt atctcaatat ggagatgcgt
    13621 ttaggtgagg ggagtggcgc agctctggcg atgcccatca tcgaagctgc ctgtgcgata
    13681 tataacaaca tgggcgaact tgctgccagt aatattgttc taccggggaa tacgacttct
    13741 gatttgaaca gttaaaaacg aaaagtctga taaaagctat acttaacgga tagctttcgc
    13801 gacttaggaa agggacatga tgcgtcgtgt aaatattttt tgctcatttg ctctgctttt
    13861 tgccagccaa aatagcctgg cggtaactta tccattacct ccagagggta gccgtttagt
    13921 ggggcagtcg cttactgtaa ctgttcctga tcacaatact cagccgctgg agacttttgc
    13981 cgcacaatac gggcaagggt taagtaatat gctggaagcg aatccgggcg ctgatgtttt
    14041 tttgccgaag cctggctcac aactcaccat tccgcagcaa ctgattttgc ccgccactgt
    14101 tcgtaaaggg attgtggtta acgtcgctga aatgcgtctt tattactatc cgccagacag
    14161 taatacagtg gaagtctttc ctattggtat cggccaggct gggcgagaaa ccccgcgtaa
    14221 ctgggtgact accgttgaac gtaaacaaga agcaccaacc tggacgccaa cgccgaacac
    14281 ccggcgcgaa tatgcgaaac gaggggagag tttgcccgca tttgttcctg cggggcccga
    14341 taaccccatg gggctgtacg cgatttatat tggcaggttg tatgccatcc acggtaccaa
    14401 tgccaatttt ggtattgggc tccgtgtaag tcagggctgt attcgtctgc gtaatgacga
    14461 tatcaaatat ctgtttgata atgttcctgt aggcactcgt gtacaaatta ttgatcagcc
    14521 agtgaaatac acaacggaac cggatggttc aaagtggctg gaagttcatg agccgctgtc
    14581 gcgcaatcgt gcagaatatg agtctgaccg aaaagtgcca ttgccggtaa ccccatcttt
    14641 gcgggcgttt atcaacgggc aagaagttga tgtaaatcgc gcaaatgctg cgttgcaacg
    14701 tcgatcggga atgcctgtgc aaattagttc tggttcaaga cagatgtttt aagagcgttg
    14761 ataattagaa agtaaaaagc ctgctcgaaa gcaggctttt ttgaatttgg ctcctctgac
    14821 tggactcgaa ccagtgacat acggattaac agtccgccgt tctaccgact gaactacaga
    14881 ggaatcgtgt gaacggggcg catattaatg gcagtgcatg gtgatgtcaa agggtgaaat
    14941 gtattttttt gtttgtttgc tgccaatttc aacaaagtgg tgagctttca atcttattgt
    15001 gtaatgaacc atcgtggtgc ataccctcct tttatagggc aggtgaatgc gactgctgat
    15061 aaaaagggcc aatgacgaat ctatcataat cttttatttg acagttggtt agcttgcgca
    15121 tcaacgccaa aatagaactg gcaagcatct tgctttctgg ttgtaagtaa tggcggcact
    15181 tgggccgatt cttaaaaacc ggaggcaaca tgaacttcag acgcctgaaa tacttcgtaa
    15241 aaattgtaga tattggtagc ctgacccagg ctgctgaagt attgcatatc gcacaaccag
    15301 cgctcagcca gcaggttgcc acactggaag gtgagttaaa tcaacagctt ttgattcgca
    15361 caaaacgggg cgttacgcca acagacgccg gaaaaattct ctatacccat gcacgggcca
    15421 ttttacgtca gtgtgagctg gcccaactgg cggtgcataa cgttggtcag tcattatcgg
    15481 ggcaagtctc gattggcttt gcgccaggaa ccgctgcgtc atccatcacc atgcccttat
    15541 tacaggcggt tcgcgctgaa ttcccggagg tcgttatcta tcttcatgaa aatagtggtg
    15601 cagtgcttaa cgaaaaactg ataaatcacc aactcgatat ggcggtgatt tatgagcatt
    15661 ccccagtggc tggggtatcc agtcaggctt tgctgaaaga agatcttttc ctagtaggaa
    15721 ctcaagattg cccggggcaa agcgttgatg ttaatgctat tgcgcaaatg aacctttttc
    15781 tccccagtga ttacagcgct gttagacttc gtgttgatga ggctttttcc ctacggcggc
    15841 tcacggcaaa agtcattggt gaaattgagt ctattgccac gcttaccgca gcgattgcca
    15901 gcggcatggg cgttgcagta ttacctgaat cggctgcgcg ttcgttatgt ggcgcagtaa
    15961 atgggtggat gtcacgcatt accactcctt caatgagtct ctctttgtcc ttaaatttac
    16021 ccgccagagc aaacttgtca ccacaggcac aggcagtgaa agagttgtta atgtcagtga
    16081 ttagttctcc agtgatggaa aaaaggcagt ggcaattggt gagctaagcg ttatatcggg
    16141 atggaataag atgcgggttt ttattatttg ttatgccggg cattagactt taacaataac
    16201 ggaaaatctg aactgcccgg agtttaccgt gaatttccaa caactaaaga taatccgcga
    16261 ggctgcacgt caggattaca acctgacaga ggttgcgaat atgcttttta cctcacagtc
    16321 cggcgtcagc cgtcatattc gggaactgga ggatgaactt ggcatcgaaa tatttgttcg
    16381 acgaggtaag cgactgctgg gcatgactga accgggcaaa gcattactgg tcattgcaga
    16441 acgtattctg aatgaagcca gtaatgttcg tcggcttgca gacctgttta ccaacgatac
    16501 gtctggcgtt ctcactattg caacgacgca tactcaggca cgttatagtt tgccagaggt
    16561 cattaaatct tttcgcgaac ttttcccgga ggttcggctc gagctaatcc aggggacgcc
    16621 acaggaaatt gcgacgttgt tgcataatgg tgaagctgat attggtatcg ccagcgagcg
    16681 tttgagtaat gacccgcagc tcgtcgcctt cccgtggttt cgttggcacc atagtttgct
    16741 tgttccactc gatcatccct tgacgcaaat tacaccattg acgctggaat caatagcgaa
    16801 gtggccgtta atcacttacc gacaggggat tacggggcgc tcacgtattg atgacgcatt
    16861 tgcccgcaaa ggtttgctgg cagatattgt attaagtgcg caggattctg atgtcattaa
    16921 aacctatgtt gctcttgggc ttgggatcgg aatagttgcc gaacaatcca gcggtgaaca
    16981 agaggaaaag aatttaatcc gcctggatac gcggcatctt tttgatgcta atactgtctg
    17041 gttgggactg aagcgaggac aacttcagcg taactatgtc tggcgctttc tggaactttg
    17101 taatgcagga ctttcagttg aggatatcaa gcggcaggtg atggaaaaca gtgaagagga
    17161 aattgattat cagatatagc aaaaagcccg ctataaaagc gggcttttca gaaatttggc
    17221 tcctctgact ggactcgaac cagtgacata cggattaaca gtccgccgtt ctaccgactg
    17281 aactacagag gaatcgctgt tgaggcacat cttaacggcg aaaaatcttt tgtcaaaccc
    17341 gatttttaat tttcccaact gtttgccgtt tctatcaaca gtttgttgca tttgcagaca
    17401 atttgttggc gaaaatcttt gcagctttgc tgattctccc tcattatttg aaatgtggtt
    17461 tcactttctg caattaaggt cggctttgaa tatctcctct gctttacgcc aggttgttca
    17521 cggcactcgc tggcacgcta aacgcaagag ctacaaagtg ttgttctggc gcgagataac
    17581 cccgcttgct gttcctatct tcatggagaa tgcctgtgtc ctgttgatgg gggttctgag
    17641 cacttttctg gtcagctggc tgggaaaaga tgcgatggcc ggcgtgggat tggcggacag
    17701 cttcaatatg gtcattatgg ctttttttgc tgctatcgat cttggtacta ctgtcgttgt
    17761 ggcatttagt ctcggtaagc gggatcgacg acgagcgagg gtggcgacgc ggcagtcatt
    17821 ggtgatcatg acgttgtttg ccgtactgtt ggcaacgctt attcatcatt ttggcgagca
    17881 aattattgat ttcgtcgcgg gtgatgccac gacagaagtt aaagcactgg cgttgactta
    17941 tctggagctg acggtactca gttatccagc agctgccatc actctaattg gtagcggggc
    18001 acttcgtggt gcagggaata cgaaaatacc gctactgatt aacggtagcc tgaatattct
    18061 taatattatt attagcggca tattgattta cggccttttc tcctggccgg gactgggatt
    18121 tgtcggggct gggttgggtt taaccatttc tcgttatatt ggcgcagttg caattttgtg
    18181 ggtgttggcg attggtttta atcctgcgct aaggatttcg ttaaagagct attttaaacc
    18241 gctgaatttt agcattattt gggaagtcat ggggattggt attcccgcga gtgtcgaatc
    18301 agtgttattt accagtggtc ggttattaac ccaaatgttc gttgccggga tggggaccag
    18361 tgttattgcc ggaaatttta tcgcgttttc aattgcggct cttatcaact tacccggaag
    18421 tgcgctcggc tctgcttcta cgatcattac aggccgaagg ttgggtgtag gacagatagc
    18481 gcaagcagag attcagttgc ggcatgtgtt ctggctttcc actcttggat taacggccat
    18541 cgcctggcta acggctccct ttgccggggt tatggcatcg ttttacaccc aggatccaca
    18601 ggttaaacat gtcgttgtga ttctgatttg gctaaatgct ttatttatgc ctatttggtc
    18661 cgcctcatgg gtgctgcccg ctggatttaa gggggctcgt gacgcccgtt acgctatgtg
    18721 ggtttctatg ttgagcatgt ggggttgccg ggttgtcgtc ggttatgtgc tgggcatcat
    18781 gcttggctgg ggtgtggttg gtgtctggat gggaatgttt gccgactggg ctgtgcgggc
    18841 cgtgctgttt tactggcgaa tggttactgg acgttggcta tggaaatacc ctcgacccga
    18901 accgcaaaag tgtgaaaaaa agccagctgt gtcggaataa acgacaaaat gcagattatt
    18961 tcagcaaacg atttcaaatt taaaaaacag gctttgacat tgtggttggg catcgctaat
    19021 attcgcctcg ttctcacgat tcctctgtag ttcagtcggt agaacggcgg actgttaatc
    19081 cgtatgtcac tggttcgagt ccagtcagag gagccaaatt ttagttttga gacatctcta
    19141 tgaatctcaa aacagttaaa aaataaaaat tatttttacc cggttacctt gtaaaaaaag
    19201 tgaccgggtt ttttgatttc tgtgtttcgt gcgtttaatc gagtctcacg cttcagtgag
    19261 caaattgccc ccaacagaca tcacgattcg caagg
//
LOCUS       DAPHCV010000094        19166 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.094, whole genome shotgun sequence.
ACCESSION   DAPHCV010000094 DAPHCV010000000
VERSION     DAPHCV010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19166
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.094"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            405..1544
                     /gene="wza"
                     /locus_tag="RH033_003436"
     CDS             405..1544
                     /gene="wza"
                     /locus_tag="RH033_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HDV2992569.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1550..1993
                     /gene="wzb"
                     /locus_tag="RH033_003437"
     CDS             1550..1993
                     /gene="wzb"
                     /locus_tag="RH033_003437"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HDV2992570.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            1996..4158
                     /gene="wzc"
                     /locus_tag="RH033_003438"
     CDS             1996..4158
                     /gene="wzc"
                     /locus_tag="RH033_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HDV2992571.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHASPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAYRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4251..5090
                     /gene="wcaA"
                     /locus_tag="RH033_003439"
     CDS             4251..5090
                     /gene="wcaA"
                     /locus_tag="RH033_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HDV2992572.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTTLNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5093..5581
                     /gene="wcaB"
                     /locus_tag="RH033_003440"
     CDS             5093..5581
                     /gene="wcaB"
                     /locus_tag="RH033_003440"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HDV2992573.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5578..6795
                     /gene="wcaC"
                     /locus_tag="RH033_003441"
     CDS             5578..6795
                     /gene="wcaC"
                     /locus_tag="RH033_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HDV2992574.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYCTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHLLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6770..7987
                     /gene="wcaD"
                     /locus_tag="RH033_003442"
     CDS             6770..7987
                     /gene="wcaD"
                     /locus_tag="RH033_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HDV2992575.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFIFRKALNITR"
     gene            7998..8744
                     /gene="wcaE"
                     /locus_tag="RH033_003443"
     CDS             7998..8744
                     /gene="wcaE"
                     /locus_tag="RH033_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HDV2992576.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQADDISLEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            8760..9308
                     /gene="wcaF"
                     /locus_tag="RH033_003444"
     CDS             8760..9308
                     /gene="wcaF"
                     /locus_tag="RH033_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HDV2992577.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATLFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLNLGDYAWVGDEVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDYASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            9335..10456
                     /gene="gmd"
                     /locus_tag="RH033_003445"
     CDS             9335..10456
                     /gene="gmd"
                     /locus_tag="RH033_003445"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HDV2992578.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10459..11424
                     /gene="fcl"
                     /locus_tag="RH033_003446"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10459..11424
                     /gene="fcl"
                     /locus_tag="RH033_003446"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HDV2992579.1"
                     /translation="MSRQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMIIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11427..11906
                     /gene="gmm"
                     /locus_tag="RH033_003447"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11427..11906
                     /gene="gmm"
                     /locus_tag="RH033_003447"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HDV2992580.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            11903..13126
                     /gene="wcaI"
                     /locus_tag="RH033_003448"
     CDS             11903..13126
                     /gene="wcaI"
                     /locus_tag="RH033_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HDV2992581.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAESVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLHNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13129..14565
                     /gene="cpsB"
                     /locus_tag="RH033_003449"
     CDS             13129..14565
                     /gene="cpsB"
                     /locus_tag="RH033_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HDV2992582.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLNFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14758..16128
                     /gene="cpsG"
                     /locus_tag="RH033_003450"
                     /gene_synonym="manB"
     CDS             14758..16128
                     /gene="cpsG"
                     /locus_tag="RH033_003450"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HDV2992583.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAHPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16184..17578
                     /gene="wcaJ"
                     /locus_tag="RH033_003451"
     CDS             16184..17578
                     /gene="wcaJ"
                     /locus_tag="RH033_003451"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HDV2992584.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGIYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17580..19058
                     /gene="wzxC"
                     /locus_tag="RH033_003452"
     CDS             17580..19058
                     /gene="wzxC"
                     /locus_tag="RH033_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HDV2992585.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVVCVAVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPVIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHSLVVEVKRQFCRSEKMKMLLRAG"
BASE COUNT         4595 a         4769 c         5309 g         4493 t
ORIGIN      
        1 aaaaatagcc aattgccgaa ttgttacctt gcctgctatt ccgttagctg taacacttcc
       61 tcctgcatta ttggagagct aatattcaat taccataatg tcaccttata ttcggaagtt
      121 aatttccgca ataacccttt ctcatcaacg attgtatgca gagagggttt taaaaattaa
      181 gctaaatcag tgtattggta gctaaaaagc caggggcggt agcgtgtctg gatgcctgaa
      241 agaccagtct gaattattct gtcaatagcc tgcggatgaa atcaattttt ttaggactga
      301 tgccagttaa atttttattc aattaactgc aaggtaaatg tgcatggcga cattgctatt
      361 aatagtgcac aggataatta ctctgccaaa gtgataaata aacaatgatg aaatccaaaa
      421 tgaaattgat gccattattg gtgtcagtaa ccttgataag cggttgcaca gtacttccgg
      481 gcagcaatat gtcgacgatg ggcaaagacg tcatcaaaca gcaggacgct gatttcgatc
      541 tcgacaaaat ggtgaatgtt tatccgctga ccccgcgcct gattgatcaa ttacgtccac
      601 gacccaacgt agcgcgcccc aatatgacgc tggaaagtga gatcgcgaat taccagtatc
      661 gcgtcgggcc gggggacgtt cttaatgtca ccgtctggga tcacccggag ctcaccacgc
      721 cagcaggtca gtaccgcagc tccagcgaca ccggcaactg ggtacagcct gacggcacca
      781 tgttctaccc gtatatcggc aaggttcacg tggtggggaa aacgctcgct gaaatccgca
      841 gtgatattac cgggcgctta gcgacgtaca tcgctgaccc gcaggtggac gttaatatcg
      901 ccgccttccg ctcacaaaag gcctatatct caggtcaggt gaataaatct ggtcaacagg
      961 cgatcaccaa cgtaccactg accattctcg acgccatcaa cgccgcaggt ggcctgaccg
     1021 acaccgctga ctggcgcaac gtggtgctaa cacacaatgg tcgtgaagag cgcatttctt
     1081 tgcaggcgct gatgcaaaac ggcgacctca accagaatcg cctgctttac cccggcgata
     1141 ttctctacgt gccgcgtaat gatgatctga aagtatttgt gatgggtgaa gtgaagaaac
     1201 agagcaccct gaaaatggac tttagcggca tgaccctgac tgaagctctg ggcaatgctg
     1261 aaggcattga catgaccacc tccaacgcca gcggcatctt tgtcattcgt ccgctgaaag
     1321 gtgagggcgg gcgtaacggc aagattgcca atatctacca gctggatatg tccgatgcca
     1381 cgtcgctggt gatggcgaca gaattccgcc tgcaacctta tgacgtggtg tatgtcacca
     1441 ccgccccggt ttcccgctgg aaccgtctga tcaatcagtt gctgccaact attagcggtg
     1501 tccgttacat gacggataca gccagcgaca ttcataactg gtaatcgtca tgtttaacaa
     1561 catcttagtt gtctgtgtcg gcaatatttg ccgttccccg acggcggaac gcttactgca
     1621 acgttatcac ccggagctaa aagtggagtc cgctggactc ggcgcgctgg tcggtaaggg
     1681 cgctgatcct accgctatca gcgtcgccgc agaacatcaa ctgtctctgg aaggccactg
     1741 tgcacgacaa atcagccgcc gcctgtgtcg caactatgac ctgattttga ccatggaaaa
     1801 gcgccatatc gaacgcttat gcgagatggc accggagatg cgcggcaaag tgatgctgtt
     1861 tggtcactgg gataacgaat gtgaaatccc cgatccgtat cgcaaaagcc gggaaacgtt
     1921 tgcagcggtg tacacattac ttgaacggtc tgcccgccag tgggcgcagg cattgaacgc
     1981 agagcaggta taagaatgac agaaaaagta aaacaacatg ccgctccggt aacgggcagt
     2041 gatgaaatcg atattggtcg cctggtcggc accgtcattg aagcgcgctg gtgggtgatt
     2101 ggcatcaccg ctgtattcgc cctttgtgcc gtggtttaca ccttcttcgc cacgccgatt
     2161 tatagtgccg acgcactggt acaaatcgag caaagcagcg gcaattcgtt agtgcaggat
     2221 atcggatcgg cgttagccaa caaaccgcct gcatcggacg ccgagatcca gttgattcgt
     2281 tcgcgcctgg tgcttggtaa aacggtggac gatctcgacc tcgatattgc ggtgagcaaa
     2341 aacacgttcc cgattttcgg tgcgggctgg gatcgcctga tgggacgtca gaacgagacg
     2401 gtgaaagtga ctacctttaa ccgtccgaaa gagatggcgg atcaggtgtt tacgcttaat
     2461 gtgctggatg acaaaaacta caccctgagc agtgatggcg gctttagcgc ccgtggacaa
     2521 gcgggccaga tgctgaaaaa agaaggcgtc acgctgatgg ttgaagccat tcacgccagc
     2581 ccgggcagtg agtttaccgt caccaaatac tccacgctgg ggatgatcaa ccaactgcaa
     2641 aacagcctga cggtaacgga gaacggcaaa gacgcaggcg tactgagcct gacttatacc
     2701 ggtgaagatc gcgaacagat ccgcgacatt cttaacagca tcgcccgtaa ctatcaggaa
     2761 caaaatattg agcgcaaatc ggcggaagcg tcgaaaagcc tcgctttcct cgcgcaacag
     2821 ttaccggaag tacgtagccg ccttgatgtt gccgaaaaca aactgaatgc ctaccgtcag
     2881 gataaagatt ctgttgatct gccgctggaa gcgaaagcgg tgctcgattc gatggtgaac
     2941 atcgacgcgc agttgaacga actgaccttt aaagaggcgg aaatctccaa gctgtacacc
     3001 aaagttcacc ccgcgtaccg cacgctgctg gagaaacgtc aggcgctgga agacgaaaaa
     3061 gccaaactta acggtcgcgt aacggcgatg ccgaaaaccc agcaggagat tgtccgtctg
     3121 acccgcgatg tcgagtctgg tcagcaggtc tatatgcaac tgctgaataa agagcaggag
     3181 ctgaaaatca ccgaagccag caccgtcggc gatgtgcgca ttgttgaccc ggcaatcact
     3241 cagcctggtg tgctaaaacc gaagaaaggg ctgattatcc ttggggcgat tattcttggc
     3301 ctgatgctct ctatcgtggg tgtgctgctg cgctcgttgt ttaatcgtgg catcgaaagc
     3361 ccgcaggtgc tggaagaaca cggtatcagc gtctatgcca gcatcccgtt gtcggaatgg
     3421 cagaaagcgc gcgatagcgt caaaaccatc aaaggagtta aacgctataa acagagccag
     3481 ctactggcgg tggggaatcc aaccgatctg gcgattgaag ccatccgcag tcttcgtacc
     3541 agtttgcact tcgcgatgat gcaggcgcag aacaatgtgt tgatgatgac cggggttagc
     3601 ccgtcaatcg gtaaaacctt tgtctgcgcc aacctggcgg cggtgatcag ccagaccaat
     3661 aaacgggtgt tgttgatcga ctgcgatatg cgcaaaggct acacccacga gctgttgggc
     3721 accaataacg tcaatggcct gtcggaaatt ctgattggtc agggcgatat tactaccgct
     3781 gctaaaccca cttcgattgc caaatttgac ctgatcccgc gcggtcaggt accgccaaat
     3841 ccttctgaac tgttgatgag cgaacgcttt gccgaactgg tgaactgggc gagtaaaaac
     3901 tacgacctgg tgttgattga tacgccgccg attctggcag tgaccgatgc ggcaattgtt
     3961 ggtcgtcatg tcggaaccac gttaatggtg gcgcgttatg cggtcaacac attgaaagaa
     4021 gtggaaacca gtctgagccg ctttgagcaa aatggtattc cggtgaaagg ggtgattctg
     4081 aactccatct tccgccgcgc cagcgcgtat caggattatg gctattacga atacgaatat
     4141 aagtcggatg cgaaataacg acgcctgcat tcgcaccgcc ccgtaggccg gataaggcgc
     4201 tcacgccgca tccggcaagt aaaccagctc ataagccggg agtacaacct atgaaagaca
     4261 atccgctgat ctcaatctat atgccaacct ggaaccgcca acaactggcg attcgggcaa
     4321 taaaatcggt tttgcgccag gactacagca actgggagat gatcatcgtg gatgattgct
     4381 ccacctcctg ggaacaactg caacagtacg tcaccaccct caacgatccg cgtatcactt
     4441 acattcacaa cgacattaac agcggggcgt gcgcggtacg taaccaggcg attatgctgg
     4501 cgcaagggga atacatcacc gggatcgatg atgatgatga atggacaccc aaccgtctga
     4561 gcgtcttcct cgcccataaa cagcaactgg tcactcacgc ctttttgtac gctaacgact
     4621 acgtttgcca gggcgaagtc tattcccaac cagcaagttt gccgctgtat cccaaatcgc
     4681 cgtattcacg ccgcctgttc tataaacgca atatcattgg caatcaggtc tttacctggg
     4741 cgtggcgttt caaagagtgt ttgttcgata ccgaactgaa agcggcacag gattacgaca
     4801 tcttcctgcg gatggtggtg gagtatggcg aaccgtggaa agtggaagag gcgacgcaga
     4861 tcctgcatat caatcacggt gaaatgcaga tcacctcgtc accgaaaaaa ttctccggat
     4921 acttccactt ttaccgcaag cacaaagaca aattcgaccg cgccagcaaa aaatatcagc
     4981 tgtttaccct ctaccagatc cgcaataaac gcatgacctg gcgcactttg ctgacgctgc
     5041 tgtcggtgcg taacggcaaa cgtctggctg atggtattcg ggggcgctaa agatgctgga
     5101 agatctgcgc gccaacagct ggagtttacg cccgtgctgc atggttcttg cctatcgcgt
     5161 cgctcatttt tgttcggtgt ggcgcaaaaa gaacgtcctc aacaatctgt gggcggcccc
     5221 gctgctggtg ctgtatcgca ttatcaccga atgctttttc ggttatgaaa tccaggctgc
     5281 cgcgaccatt ggccgccgct ttactatcca tcacggttac gccgtagtga tcaataaaaa
     5341 cgtggtagcg ggggatgatt ttaccattcg ccacggcgtc actatcggca atcgtggtgc
     5401 cgataacatg gcatgtccac acattggcaa cggcgtcgaa ctcggtgcca acgtcattat
     5461 tcttggtgat atcacgcttg gtaacaacgt caccgtgggc gcgggtagcg tggtgctcga
     5521 ctctgtcccg gacaacgcgc tggtggtggg agaaaaagcg cgagtgaagg taattaaatg
     5581 aatattttgc aatttaatgt gcgactggcg gaaggcgggg cagcaggtgt ggcgttagat
     5641 ctccaccagc gtgcgctgca acaggggctg gcgtcacatt ttgtctacgg ttacggcaaa
     5701 ggcggcaaag agagtgtcag ccatcaaaac tatccgcagg tcatcaaaca tacgccgcgg
     5761 atgactgcaa tggcgaatat tgccctgttt cgtctgttta atcgcgatct gtttggcaat
     5821 ttcaatgagt tatattgcac cattactcgt acaccgggtc cggtggtcct gcattttcat
     5881 gtgctgcaca gctactggct aaatcttaag agcgtggtgc gcttttgcga aaaggtgaaa
     5941 aaccacaaac cggatgtcac tctggtctgg acgctgcacg accactggag tgtaaccgga
     6001 cgctgcgcct ttaccgacgg ttgcgaaggc tggaaaacgg gctgccagaa atgcccgacc
     6061 ttaaataact atccgccggt gaagattgat cgggcgcacc tgctggtggc gggcaaacgc
     6121 cagttattcc gtgagatgct ggcgctgggc tgtcagttta tttcccccag ccagcatgtg
     6181 gctgacgctt tcaatagcct gtacggtcca gggcgttgcc ggattatcaa taacggtatt
     6241 gatatggcaa ccgaagcgat tctggcggat ctgcctccgg tgcgcgaaac tcagggcaag
     6301 ccgaaaatcg cggtggtggc gcatgacctg cgttacgacg gcaagactaa ccagcaactg
     6361 gtgcgtgaga tgatggcgct gggcgacaaa attgaactgc atacctttgg taagttctcg
     6421 ccgttcaccg ctggcaacgt ggttaatcac ggctttgaaa ctgacaagcg caagttgatg
     6481 agcgcgctca atcagatgga tgcgctggtg ttcagttctc gcgtcgataa ctacccgctg
     6541 attttgtgtg aggcgttatc aattggcgtg ccggtgattg ccacccatag cgacgcggcg
     6601 cgtgaagtgt tgcaaaaatc cggcggtaaa accgtcagcg aagaagacgt gctgcaactg
     6661 gtccagttaa gcaaaccgga aatcgcgcag gcgatatttg gtaccacgct ggctgagttc
     6721 agccaacgca gccgcgcggc ctacagtgga caacagatgc tggaggagta tgtcaacttc
     6781 tatcagaatc tgtagctacc tgctgctgcc gctgatctac ctgctggtta acgtcaaaat
     6841 cgcccagctt ggcgaaagtt ttcccatcac catcgtcact tttttacctg tcttgttact
     6901 gctgttttta gaacgcatca gcgttaaaaa attgatgatt gccttaggga ttggcgcggg
     6961 actcacggcg tttaactacc tgtttggtca gtcgttggat gccagtaaat acgtcacttc
     7021 aaccatgctg tttgtctata ttgtgatcat tattggcatg gtgtggagta ttcgctttaa
     7081 aacgatttcg ccacacaacc atcgtaagat attacgtttc ttttatctgg tggtcgggct
     7141 ggtggtggcg ctggcggcgg tggagatggc gcaaattatc ctcaccggtg gcagcagtat
     7201 tatggagtcg atttcgaaat atctgattta cagcaatagc tatgtgctga atttcatcaa
     7261 attcggcggc aagcgcacga cagcacttta tttcgaaccg gcatttttcg ctctggcatt
     7321 aatctcaatt tggctcagca tcaaacagtt tggtatcaaa acgcctaaaa ccgatgctat
     7381 gattctcgca gggataatat tatccggatc gttttcaggg gttatgacct ttatcctgtt
     7441 ttatttgctg gagtgggcat ttcaatatct gaataaagag gcgattaaga aaaagttacc
     7501 gttagcattg atttctctgg ctgtattcct ggttggtgtg gtaatcgcgt ttccttatat
     7561 ttccacccgt ctgggcgatt taggtacgga aggatcgtca tcgtattatc gtattgtcgg
     7621 tccgctggtg atggtcggtt attctttgac ccatattgac ggtgtagtca gatttggctc
     7681 actttatgaa tatgttgcat cattcggaat atttaacggt gcggatgtcg gaaaaaccat
     7741 agacaatggt ttgtatctgc tgattattta tttttcctgg ttcgcggtgt ttttatcgct
     7801 gtggtacatg gggaaagtga taaaaatgat gatcaacgct tttggtgata accgcaattt
     7861 tcgcgtgcaa ttatatcttt ttactccggt atcgctgttt tttaccggtt cgatatttag
     7921 cccggaatat gcatttttaa tcgtctgtcc gtttattttt cgaaaagcgt taaatattac
     7981 gaggtaagaa taagaacatg ttgcttagca taatcactgt cgcgtttcgt aacctcgaag
     8041 ggatagtcaa aacacatgcc tcgctggcgc atctggcgca ggcggacgat atcagcctcg
     8101 aatggattgt tgtcgatggc ggttccaacg acggtacccg tgagtatctg gaaaatctca
     8161 atggtatcta taatctacgc tttgtcagcg agccagataa cggtatctac gacgccatga
     8221 acaaaggcat tgcgatggca caaggcaagt tcgcgttgtt tctcaactcg ggcgatattt
     8281 ttcatcagga tgccgcaaat tttgtccgta agttaaaaat gcaaaaagat aacgtgatga
     8341 tcaccggcga tgcgctgctg gattttggcg acgggcataa aattaagcgt agcgccaaac
     8401 cgggttggta tatttatcac agcctgcccg ccagtcatca ggcgatattt ttcccggtat
     8461 ccggcttgaa gaaatggcgt tatgacctgg aatataaagt ttcttccgac tacgcgctgg
     8521 cggccaaaat gtataaagcc ggttatgcat ttaaaaaact caatggcctg gtgtctgaat
     8581 tttccatggg tggggtatct accaccaata atatggaatt gtgtgctgac gcgaaaaaag
     8641 tccaacggca aatattacat gtgcctggct tttgggctga attatcctgg catttacgcc
     8701 aacgtactac ctcaaagacg aaagccttat ataacaaaag ctgaatataa gggaaaacga
     8761 tgcaagattt aagcggtttc tcggtgccga aagggttccg gggtggcaac gctattaaag
     8821 tgcaattgtg gtgggcagta caggcaacat tatttgcctg gtcgccacaa gtattgtatc
     8881 gctggcgggc atttttatta cgtttattcg gggcaaaaat aggaaaaaac gtagttattc
     8941 gtccgtcagt aaaaattacc tatccgtgga aattaaactt aggtgattac gcgtgggtcg
     9001 gcgatgaggt caatttatat accctcggtg aaataaccat tggcgcacat tcggtgatat
     9061 cgcaaaaaag ttatttatgc accgggagcc acgactatgc aagtcaacat ttcaccatta
     9121 acgccacgcc tattgtgatt ggcgagaaat gctggctggc aaccgatgtc tttgttgccc
     9181 caggcgtcac aatcggcgac ggcaccgtcg tgggtgcacg aagcagtgtt tttaaatcgc
     9241 ttccggcaaa tgtggtttgc cgggggaatc ccgcagtggt gatacgcgaa cgcgttgaaa
     9301 ctgaataaat tcaaaaaata cagaggaata agacatgtca aaagtcgctc tcatcaccgg
     9361 tgtaaccgga caagacggtt cttacctggc agagtttctg ctggaaaaag gttacgaggt
     9421 gcatggtatt aagcgtcgcg catcgtcatt caacaccgaa cgcgtggatc acatttatca
     9481 ggatccgcac acctgcaacc cgaaattcca tctgcattat ggcgacctga gtgatacctc
     9541 caacctgacg cgcattttgc gtgaagtgca gccggatgaa gtctacaacc tgggcgcaat
     9601 gagccacgtt gcggtttctt ttgagtcacc ggaatatacc gctgacgtcg acgcgatggg
     9661 tacgctgcgc ctgctcgagg cgatccgctt cctcggtctg gaaaagaaaa cccgtttcta
     9721 tcaggcttcc acctctgaac tgtatggtct ggtgcaggaa attccgcaga aagaaaccac
     9781 gccgttctac ccgcgatctc cgtatgcggt cgccaaactg tacgcctact ggatcaccgt
     9841 taactaccgc gaatcctacg gcatgtacgc ctgcaacggt attctcttca accatgaatc
     9901 cccgcgccgt ggcgaaacct tcgttacccg caaaattacc cgcgcaatcg ccaacatcgc
     9961 ccaggggctg gagtcgtgcc tgtacctcgg caatatggat tccctgcgtg actggggcca
    10021 tgccaaagac tacgtaaaaa tgcagtggat gatgctgcaa caggaacagc cagaagattt
    10081 cgttatcgcg accggcgttc agtactccgt acgtcagttc gtggaaatgg cggcggcaca
    10141 gctgggcatc aaactgcgct ttgaaggcac gggcgttgaa gagaagggca ttgtggtttc
    10201 cgtcaccgga catgacgcgc cgggcgttaa accgggtgat gtgattatcg ccgttgaccc
    10261 gcgttacttc cgtccggctg aagttgaaac gctgctcggc gacccgacca aagcgcacga
    10321 aaaactgggc tggaaaccag aaatcaccct cagagagatg gtgtctgaaa tggtggctaa
    10381 tgacctcgaa gcggcgaaaa aacactctct gctgaaatct cacggctacg acgtggcgat
    10441 cgcgctggag tcataagcat gagtagacaa cgcattttta ttgctggcca tcgcgggatg
    10501 gtcggttctg ccatcaggcg gcagctcgaa cagcgcggtg atgtggaact ggtattacgc
    10561 acccgcgacg agctgaacct gctggacagc cgcgccgtgc atgatttctt tgccagcgaa
    10621 cgtattgacc aggtctatct ggcggcggcg aaagtgggcg gcattgttgc caacaacacc
    10681 tatccggcgg atttcatcta tcagaacatg ataattgaga gcaacatcat tcacgccgcg
    10741 catcagaacg acgtgaacaa actgctgttt ctcggatcgt cctgcatcta cccgaaactg
    10801 gcaaaacagc cgatggcaga aagcgagtta ttgcagggca cgctggagcc gaccaacgag
    10861 ccttacgcca ttgccaaaat cgccgggatc aaactgtgcg aatcatacaa ccgccagtac
    10921 ggacgcgatt accgctcagt catgccgacc aacctgtatg gcccgcatga caacttccac
    10981 ccgagtaatt cgcatgtgat ccctgcattg ctgcgtcgct tccacgaggc gacggcacag
    11041 aatgcaccgg acgtggtggt atggggcagc ggtacaccga tgcgtgaatt cctgcacgtc
    11101 gatgatatgg cggcggcgag cattcatgtc atggagctgg cgcatgaagt ctggctggag
    11161 aacacccagc cgatgctgtc gcacattaac gtcggcacgg gcgttgactg cactatccgc
    11221 gagctggcgc aaaccatcgc caaagtggtg ggttacaaag gtcgggtggt ttttgatgcc
    11281 agcaagccgg atggtacgcc gcgcaaactg ctggatgtga cgcgcctgca tcagcttggc
    11341 tggtatcacg aaatctcact ggaagcgggg cttgccagca cttaccagtg gttccttgag
    11401 aatcaagacc gctttcgggg gtaatgatgt ttttacgtca ggaagacttt gccacggtag
    11461 tgcgctccac tccgcttgtc tctctcgact ttatagtcga gaacagtcgc ggcgagtttc
    11521 tgcttggcaa aagaaccaac cgcccggcgc agggttactg gtttgtgccg ggagggcgcg
    11581 tgcagaaaga cgaaacgctg gaagccgcat ttgagcggct gacgatggcg gaactggggc
    11641 tgcgtctgcc gataacagca ggccagtttt acggtgtctg gcagcacttt tatgacgata
    11701 acttctctgg cacggatttc accactcact atgtggtgct cggttttcgc ttcagagtag
    11761 cggaagaaga actgttactg ccggatgagc agcatgacga ttaccgctgg ctgacgccgg
    11821 acgcactgct cgccagtgac aatgttcacg ctaacagccg cgcctatttt ctcgctgaga
    11881 agcgtgccgg agtacccgga ttatgaaaat actggtctac ggcattaact actcgccgga
    11941 gttaaccggc atcggcaaat acaccggcga gatggtggaa tggctggcgg cacaaggtca
    12001 tgaggtgcgg gtcattaccg caccgcctta ctacccgcag tggcaggtgg gcgagaacta
    12061 ttccgcctgg cgctacaaac gagaagaggg ggccgccacg gtgtggcgct gcccgctgta
    12121 tgtgccaaaa cagccgagca ccctgaaacg cctgttacat ctcggcagtt ttgccgtcag
    12181 cagttttttc ccgttgatgg cgcaacgtcg ctggaagccg gatcgcatta tcggcgtggt
    12241 gccaacgctg ttttgcacgc cgggaatgcg cctgctagcg aaactctctg gcgcgcgtac
    12301 cgtgctgcat attcaggatt acgaagtaga tgccatgctg gggctgggcc ttgccggaaa
    12361 aggcaaaggc ggcaaagtgg cacagctggc aacggcgttc gaacgtagcg gactgcataa
    12421 cgtcgataac gtctccacga tttcgcgttc gatgatgaat aaagccatcg aaaaaggcgt
    12481 ggcggcggaa agcgtcatct tcttccccaa ctggtcggaa atcgcccgtt ttcagcatgt
    12541 tgcagacgcc gatgttgacg cccttcgtaa ccagcttggc ctgccggata acaaaaaaat
    12601 cattctttac tccggcaata ttggtgaaaa gcaggggctg gaaaacgtta ttgaagcagc
    12661 cgatcgcctg cgcgatgaac cgctgatttt tgccattgtc gggcagggag gcggcaaagc
    12721 gcggctggaa aaaatggcgc agcagcgtgg actgcacaac atgcaattct tcccgctgca
    12781 atcgtatgac gctttacccg cactgctgaa gatgggcgat tgccatctgg tggtgcaaaa
    12841 acgcggcgcg gcagatgccg tattgccgtc gaaactgacc aatattctgg cggtaggcgg
    12901 taacgcagtg attaccgcag aggcccacac agaactggga cagctttgcg aaacctttcc
    12961 gggcattgcg gtttgcgtag aaccggaatc ggtcgaggcg ctggtggcgg ggattcgtca
    13021 ggcgctcctg ctgcccaaac acaacacggt ggcacgtgaa tatgccgaac gcacgctcga
    13081 taaagagaac gtgttacgtc aatttataaa tgatattcgg ggataattat ggcgcagtcg
    13141 aaactctatc cagttgtgat ggcaggtggc tccggtagcc gcttatggcc gctttcccgc
    13201 gtactttatc ccaagcagtt tttatgcctg aaaggcgatc tcaccatgct gcaaaccacc
    13261 atctgccgcc tgaacggcgt ggagtgcgaa agccctgtgg tgatttgcaa tgagcagcac
    13321 cgctttattg tcgcggaaca gctgcgtcaa ctgaacaaac tcaccgagaa cattattctc
    13381 gaaccggcag ggcgtaacac tgcacctgcc attgcactgg cggcgctggc ggcaaaacgt
    13441 catagcccgg agagcgaccc gttaatgctg gtattggcgg cggatcatgt gattgccgat
    13501 gaagacgcgt tccgtgccgc cgtgcgtaat gccatgccat atgccgaagc gggcaagctg
    13561 gtgaccttcg gcattgtgcc ggatctacca gaaaccggtt atggctatat tcgtcgcggt
    13621 gaagtgtctg cgggtgagca ggatacggtg gcctttgaag tggcgcagtt tgtcgaaaaa
    13681 ccgaatctgg aaaccgcgca ggcctatgtg gcaagcggcg aatattactg gaacagcggc
    13741 atgttcctgt tccgcgccgg acgctatctc gaagaactga aaaaatatcg cccggatatc
    13801 ctcgatgcct gcgaaaaagc gatgagcgcc gtcgatccgg atctcaattt tattcgcgtg
    13861 gatgaagaag cgtttctcgc ctgcccggaa gagtcggtgg attacgcggt catggaacgt
    13921 acggcagatg ccgttgtggt gccgatggat gcgggctgga gcgatgtcgg ttcctggtcc
    13981 tctttatggg agatcagtgc ccacaccgcc gagggcaacg tttgccacgg cgatgtgatt
    14041 aatcataaaa ctgaaaacag ctatgtgtac gccgaatctg gcctggtcac caccgtcggg
    14101 gtgaaagatt tggtagtggt gcagaccaaa gatgcggtgc tgattgccga ccgtaacgcg
    14161 gtgcaggatg tgaaaaaagt ggtcgagcag atcaaagccg atggtcgcca tgagcatcgg
    14221 gtgcatcgcg aagtgtatcg tccgtggggc aaatatgact ctatcgacgc gggcgaccgc
    14281 taccaggtga aacgcatcac cgtgaaaccg ggtgagggct tgtcggtaca gatgcaccat
    14341 caccgcgcgg aacactgggt agtggtcgcg ggaacggcaa aagtcaccat tgatggtgat
    14401 atcaaactgc ttggtgaaaa cgagtccatt tatattccgc tgggggcgac gcactgcctg
    14461 gaaaacccgg ggaaaattcc gctcgattta attgaagtgc gctccggctc ttatctcgaa
    14521 gaggatgatg tggtgcgctt cgcggatcgc tacggacggg tgtaaacgtc gcatcaggag
    14581 ctgtttgtcg gatgcggcgt gaacgcctta tccgacctac ggttcgggtt tgtagtcctg
    14641 ataagacgcg gcagcgtcgc atcaggcaat gaatgcgaaa ccgagatgta aataacgaca
    14701 aaaacaaaat tggccgtttc ggtcagggcc aactattgcc tgaaaaaggg taacgacatg
    14761 aaaaaattaa cctgctttaa agcctatgat attcgcggga aattaggcga agaactgaat
    14821 gaagatatcg cctggcgcat tggtcgcgcc tatggcgaat ttctcaaacc gaaaaccatt
    14881 gtgttaggcg gtgatgtccg tctcaccagc gaaaccttaa aactggcgct ggcaaaaggt
    14941 ttacaggatg cgggcgttga cgtgctggat attggtatgt ccggcaccga agagatctat
    15001 ttcgccacgt tccatctcgg cgtggatggc ggcattgaag ttaccgccag ccataatccg
    15061 atggattata acggcatgaa gctggttcgc gagggggctc gcccgatcag cggagatacc
    15121 ggactgcgcg acgtccagcg tctggctgaa gccaacgact tccctcccgt cgatgaaacc
    15181 aaacgcggtc gctatcagca aattaacctg cgtgacgcat atgttgatca cctgttcggt
    15241 tatatcaatg tcaaaaacct cacgccgctc aagctggtga tcaactccgg gaatggcgcg
    15301 gcgggtccgg tggtggacgc cattgaagcc cgctttaaag ccctcggcgc acccgtggaa
    15361 ttgatcaaag tacacaacac gccggacggc aatttcccca acggtattcc taatccattg
    15421 ctgccggaat gccgcgacga tacccgcaat gcggtcatca aacacggcgc ggatatgggc
    15481 attgcttttg atggcgattt tgaccgctgt ttcctgtttg acgaaaaagg gcagtttatc
    15541 gagggctact acattgtcgg cctgctggca gaagcgttcc tcgaaaaaaa tcccggcgcg
    15601 aagatcatcc acgatccgcg tctctcctgg aacaccgttg atgtggtgac tgccgcaggt
    15661 ggcacgccgg taatgtcgaa aaccggacac gcctttatta aagaacgtat gcgcaaggaa
    15721 gacgccatct acggtggcga aatgagcgct caccattact tccgtgattt cgcttactgc
    15781 gacagcggca tgatcccgtg gctgctggtc gccgaactgg tgtgcctgaa agggaaaacg
    15841 ctgggcgaac tggtgcgcga ccggatggct gcgtttccgg caagcggtga gatcaacagc
    15901 aaactggcgc accccgttga ggcgattaac cgcgtcgaac agcattttag ccgcgaggca
    15961 ctggcggtgg atcgtaccga tggcatcagc atgacctttg ccgactggcg ctttaacctg
    16021 cgctcctcca acaccgagcc ggtggtgcgg ttgaatgtgg aatcacgcgg tgatgtgccg
    16081 ctgatggaag cgcgaacgcg aactctgctg acgttgctga acgagtaatg tcggatcttc
    16141 ccttacccca cttgcgggta aggggctaaa aacaggaaca acgatgacaa atctaaaaaa
    16201 gcgcgagcga gcgaaaacca atgcatcgtt aatctctatg gtgcaacgct tttcagatat
    16261 caccatcatg tttgccggac tatggctggt ttgcgaagtc agcggactgt cattcctcta
    16321 catgcacctg ttggtggcgc tgattacgct ggtggtgttc cagatgctgg gcggcatcac
    16381 cgatttttat cgctcatggc gcggtgttcg ggcagcgaca gaatttgctc tgttgctaca
    16441 aaactggacg ttaagcgtga ttttcagcgc cggactggtg gcgtttaaca atgatttcga
    16501 cacgcaactg aaaatctggc tggcgtggta tgggctgacc agcatcggac tggtggtttg
    16561 ccgttcgtgt attcgcattg gggcgggctg gctgcgtaat catggctata acaaacgcat
    16621 ggtcgccgta gcgggggatt tagccgccgg acaaatgctg atggagagct tccgtaatca
    16681 gccgtggtta gggtttgaag tggtgggtat ataccacgac ccgaaaccgg gcggcgtttc
    16741 taacgactgg gcgggtaact tgcaacagct ggtcgaggat gcaaaagcgg gaaagattca
    16801 taacgtctat atcgcgatgc aaatgtgcga cggcgcgcga gtgaaaaaac tggtccatca
    16861 attggcggac accacctgtt cggtgctgct gatccccgat gtctttacct tcaacattct
    16921 ccattcacgt ctcgaagaga tgaacggcgt tccggtggtg ccgctttacg atacgccgct
    16981 ttccggggtt aaccgcctgc taaaacgtgc ggaagacatt gtgctggcga cgcttattct
    17041 gctgctgatc tccccggtgc tgtgctgcat tgcgctggcg gtgaaactca gttcacctgg
    17101 gccggttatt ttccgccaga ctcgctacgg catggatggc aagccgatca aagtgtggaa
    17161 gtttcgttcc atgaaagtga tggagaacga caaagtggtg acccaggcga cgcagaacga
    17221 tccgcgcgtc actaaagtgg ggaactttct gcgccgcacc tcgctggatg aactgccgca
    17281 gtttatcaat gtgctgaccg gcgggatgtc gattgtgggg ccacgtccac acgcggtggc
    17341 gcataacgaa cagtatcgac agctcattga aggctacatg ctgcgccata aggtgaaacc
    17401 gggcattacc ggctgggcgc agattaacgg ctggcgcggc gaaaccgaca cactggagaa
    17461 aatggaaaaa cgcgtcgagt tcgaccttga gtacatccgc gaatggagcg tctggttcga
    17521 tatcaaaatc gttttcctga cggtattcaa aggtttcgtt aacaaagcgg catattgata
    17581 tgagcttacg tgaaaaaacc atcagcggcg cgaagtggtc ggcgattgct acggtgatca
    17641 tcatcggcct cgggctggta cagatgaccg tgctggcgcg gattattgac aaccaccagt
    17701 tcggcctgct taccgtgtcg ctggtgatca tcgcgctggc agatacgctt tctgacttcg
    17761 gtatcgctaa ctcgattatt cagcgaaaag aaatcagcca ccttgaactc accacgctgt
    17821 actggctgaa cgtcgggctg gggatcgtgg tgtgcgtggc ggtgtttttg ttgagtgatc
    17881 tcatcggcga cgtgctgaat aacccggacc tggcaccgtt gattaaaaca ttgtcgctgg
    17941 cgtttgtggt gatcccccac gggcaacagt tccgcgcgtt gatgcaaaag gagctggagt
    18001 tcaacaaaat cggcatgatc gaaaccagcg cggtactggc gggctttact tttacggtgg
    18061 ttagcgccca tttctggccg ctggcgatga ccgcgattct cggttatctg gtcaatagtg
    18121 cggtgagaac gctgctgttt ggctactttg gccgcaaaat ttatcgcccc ggtctgcatt
    18181 tctcgctggc gtcggtggca ccgaacttac gctttggtgc ctggctgacg gcggacagca
    18241 tcatcaacta tctcaacacc aacctttcaa cgctggtgct ggcgcgtatt ctcggcgcgg
    18301 gcgtggcagg gggatacaac ctggcgtaca acgtggcagt tgtgccgccg atgaagctga
    18361 acccaatcat cacccgcgtg ttgtttccgg catttgccaa aattcaggac gacaccgaaa
    18421 agctgcgcgt taacttctac aagctgctgt cggtggtggg gattatcaac tttccggcgc
    18481 tgctcgggct gatggtggtg tcgaataact ttgtaccgct ggtatttggc gaaaagtgga
    18541 acagcattat tccggtgctg caattgctgt gtgtggtggg tctgctgcgt tctgtgggta
    18601 acccgattgg ttcgctgctg atggcgaaag cgcgggtcga tatcagcttt aaattcaacg
    18661 tattcaaaac ctttctgttt attccggtga ttgttattgg tgggcagatg gcgggcgcaa
    18721 tcggcgtcac gcttggcttc ctgctggtgc agattatcaa caccattctg agttacttcg
    18781 tgatgattaa accggtgctc ggttccagtt atcgtcagta catcctgagt ttgtggctgc
    18841 cgttttatct ctcgctgccg acactggtgg tcagttatgc gctgggcatt gtgctgaaag
    18901 ggcaactggc gttgggcatg ctgctggcgg tgcaaatagc cgcgggcgtg ctggcgtttg
    18961 tggtgatgat tgtgctgtcg cgccattcgc tggtggtgga agtgaagcgt cagttttgtc
    19021 gcagcgaaaa aatgaaaatg cttttacggg cggggtgaat ggttgttttc ggttccgaca
    19081 acggttgccg gatgcggcgt aaaacgcctt atccgtttgg gggggctggg cttggatctt
    19141 gtaggcctga taagacgcgt tagcgt
//
LOCUS       DAPHCV010000095        19047 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.095, whole genome shotgun sequence.
ACCESSION   DAPHCV010000095 DAPHCV010000000
VERSION     DAPHCV010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.095"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            24..1369
                     /locus_tag="RH033_003453"
                     /pseudo
     CDS             24..1369
                     /locus_tag="RH033_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310036.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            1538..2143
                     /locus_tag="RH033_003454"
     CDS             1538..2143
                     /locus_tag="RH033_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDV2992586.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLHKRQVSANQITLAALALSLLTGL
                     LLALVPYHGMFLLLPIVLFVRMALNALDGMMARECNQQTRHGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGIRSYAGPFGKSDRALIFGLWVL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMNAEK"
     gene            2143..3039
                     /locus_tag="RH033_003455"
     CDS             2143..3039
                     /locus_tag="RH033_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDV2992587.1"
                     /translation="MLEKSLATLFALLILAMLINRFLVWRLPERKGDEVTLRIRTWWG
                     IVICFSLVISGPRWVTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLLIGLNC
                     FELFLLFIPLFGFLILATWRVFVGEPSGFLHTVSAIFCGWIMTVFALSHTAWLLMLPT
                     TNIQGEALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     VSLIIGPLLTPLDTLQALLAGLLIGISGFCGDVVMSAIKRDVGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            3055..4812
                     /locus_tag="RH033_003456"
     CDS             3055..4812
                     /locus_tag="RH033_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HDV2992588.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGQTSGPRGYSPSLASSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVMVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIISDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQYEDRTGPSADRWRLLSGGAVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLVDKIYLNSVGWRGIRQRKTHLQ
                     MLIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDIFLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIDPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            4802..6118
                     /locus_tag="RH033_003457"
     CDS             4802..6118
                     /locus_tag="RH033_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HDV2992589.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFIPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATIMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMMPVDRRWNYQKPDQRRIK
                     IALPYVVGACSCIVLMELMIMLQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPCRIGMWLSMRWFCLRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREKQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYLRARRPQIVLTDEHKAMLRLWENR"
     gene            complement(6172..6777)
                     /gene="azoR"
                     /locus_tag="RH033_003458"
     CDS             complement(6172..6777)
                     /gene="azoR"
                     /locus_tag="RH033_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDV2992590.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPNDASLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            6978..10880
                     /gene="hrpA"
                     /locus_tag="RH033_003459"
     CDS             6978..10880
                     /gene="hrpA"
                     /locus_tag="RH033_003459"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HDV2992591.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDHAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESSGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELIIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            11152..11952
                     /locus_tag="RH033_003460"
     CDS             11152..11952
                     /locus_tag="RH033_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDV2992592.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEMTYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFVNPLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIQAWQTLKHDAVLIEAMESRSLR"
     gene            12149..13588
                     /gene="aldA"
                     /locus_tag="RH033_003461"
     CDS             12149..13588
                     /gene="aldA"
                     /locus_tag="RH033_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDV2992593.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISTLIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(13629..14630)
                     /gene="gap"
                     /locus_tag="RH033_003462"
     CDS             complement(13629..14630)
                     /gene="gap"
                     /locus_tag="RH033_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDV2992594.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILTYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILAKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            14819..15349
                     /gene="cybB"
                     /locus_tag="RH033_003463"
     CDS             14819..15349
                     /gene="cybB"
                     /locus_tag="RH033_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HDV2992595.1"
                     /translation="MGNKYSRLQISIHWLVFLLVIVAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGMTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            15594..15767
                     /locus_tag="RH033_003464"
     CDS             15594..15767
                     /locus_tag="RH033_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992596.1"
                     /translation="MKKLALILVIGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCIN"
     gene            complement(15839..15988)
                     /gene="hokB"
                     /locus_tag="RH033_003465"
     CDS             complement(15839..15988)
                     /gene="hokB"
                     /locus_tag="RH033_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HDV2992597.1"
                     /translation="MKRNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            16387..18027
                     /gene="trg"
                     /locus_tag="RH033_003466"
     CDS             16387..18027
                     /gene="trg"
                     /locus_tag="RH033_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HDV2992598.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMESMKRNIAQAESE
                     IKQSQQGYRAYQNRSVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILSKAVKILSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHVAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(18065..18988)
                     /locus_tag="RH033_003467"
     CDS             complement(18065..18988)
                     /locus_tag="RH033_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2992599.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPDHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
BASE COUNT         4759 a         4362 c         4978 g         4948 t
ORIGIN      
        1 ttttttaatg gcatggagtg catatgttta agcccaggat ttgcagctgg ataggtttac
       61 ttcccctgtt tatgctgagt cttccagtac aggcggaact tcgatgtgtt gtaaatgcag
      121 ttgatattga accatttttt tctgcggcta ccgctgaaga taaacaacaa gttgaacaag
      181 ctatcaacag tagcgtgaat cttgtccccg tcggtttatc tgcatcggac tggaaagtgc
      241 atcgtggcga tttaatggta gaaggtaata tagagagtaa tcaaaaattg attgttcttg
      301 gcaatttgac agtcaaaggt aatatttcca ctttctctct ttctaatcca tgggttattc
      361 tcggtaacgt aacgtaacgt aacgtaacgt aacgtaacgt aacgtaacgt aacggcaacg
      421 aatatagtta cggattcacc actgttaatt accggttcga taaatgcaag tggactggta
      481 tttatcgact catattacga taatccgtct acgattaagg gaagtattaa tgcgcgcggg
      541 atatttatca atgacataat tgcacctgtt gttgcatctt cgacaaatag tgaattcatg
      601 gtccgtgcga gtgacaaaaa tgacactgaa aatgtcaaaa aagcgctgat gataataaat
      661 cctgatgcat attattgggg cctaataaat gatgaagatg ctctgaaaga aatttttaag
      721 cgaagcaata ttcgcatagc agggaatgtc tgtaatcaga tgaaaaaaga agcgctgttt
      781 cgccctaagt cttctcctga attattgcag gaattgcaaa tgctggatga aggcaacgtt
      841 gctgcatttg aaggacgaga cattgcgaca tttgatcttg ccataatgcg aactctccca
      901 aggcttaaag gaattacagc aaaacacgcc acgaaatgat atttgggcga agttttttct
      961 gaggcaggaa aacagttccc gtgcgcaggt taacgaagcg ttacgtgttt attatgcact
     1021 agaacccgat gcgttagcac aactggatgt actggcaaaa caaccggatc gtatatggtg
     1081 gagtacactg gcgaaaagca atctcacatt tttcaagttc ggcgcactta acaaccgcca
     1141 cacaccgcct gcagtactgg cggcagaaat tgatcccgag tggtggattg tggcgatgaa
     1201 taatccccgt tttccagttg atgtattaaa ggcgaggctg aaacgtgatc ctttgctggc
     1261 gttagagctt gttaaccctg aactggattt agtccgccag ctggcgctta acggtaagac
     1321 gcgcgcaata cgggaacaag cgatgagaaa acttgatgag ttgcattgaa taacagcgta
     1381 atatttttaa ctatttgatt aattggggat aatcattcct gacagtgagc cccaatacct
     1441 tgatatattc tgaattttta atgaagcggc gtgttgcgat atctctgcca gggcaattga
     1501 tgcaccatag cgcaaacaga attatcaagg attgataatg acgctctacc agataaaacc
     1561 gctctttcag tcactgttaa ggccgacgat gttttggctt cataagcggc aagtgtctgc
     1621 aaatcagatc acccttgccg cgttggcact ttctcttctc accggtttgt tgttggcgtt
     1681 ggtgccatat catggcatgt ttctgctatt gcccatcgtg ctttttgtcc gtatggcact
     1741 caatgcgctg gatggcatga tggcgcgtga gtgcaatcag caaacacgtc atggggcgat
     1801 tttgaatgaa actggcgatg ttatttccga tattgcgctt tatttaccgt ttttattttt
     1861 accggaaagt aacgcatcac tcgtaatact catgttattt tgcaccatat tgactgagtt
     1921 ttgcggttta ctcgcgcaga cgattaacgg tattcgcagt tatgccggtc catttggcaa
     1981 aagcgatcgc gcgttaatat ttgggttgtg ggtgctggcc gttgccattt atccacagtg
     2041 gatgcaatgg aataatcttt tatggagcat tgcttcaatt ctgcttctct ggactgcgat
     2101 taatcgttgt cggagtgtgc tcctgatgaa cgctgaaaaa taatgctgga aaaatctctg
     2161 gcaacacttt tcgcgttgtt aattttagca atgctgataa accgttttct ggtatggcgg
     2221 ttaccggaga gaaaagggga tgaagttaca ttacgtattc gtacctggtg gggcatcgtc
     2281 atttgttttt cactggtgat ttcaggtcca cgctgggtga cgttgacgtt ttttgcgctg
     2341 ataagttttc tggcattgaa agaatattgt acgcttatat ccgtacattt tccgcgttgg
     2401 ttatattggg ttattcctct taactatttg cttatcggtt taaattgctt tgagctgttt
     2461 ctgttattta tacctttgtt tgggtttctg atattagcca cctggcgagt ttttgtgggt
     2521 gaaccctccg gttttctgca taccgtgagc gccatttttt gtggctggat aatgaccgtt
     2581 ttcgccttga gtcataccgc ctggttatta atgttgccaa ccacaaatat ccagggcgag
     2641 gcgttactgg tgttatttct tttggcatta accgagtcaa acgatattgc acagtattta
     2701 tgggggaaat cctgcggcag aagaaaagtg gtccccaaag tcagcccggg aaaaacgctg
     2761 gaaggtctgg tggggggcgt aatcactacc atgattgtgt cactgattat tggaccgtta
     2821 ctgacaccgc tagatacatt acaggcatta ttagcgggtt tgttaattgg tattagtggt
     2881 ttttgcggcg atgtagtgat gtcagccatc aaacgagatg ttggtgttaa agatagtgga
     2941 aaactattac caggacatgg cggactcctt gacaggattg actcattaat tttcaccgct
     3001 ccggtatttt tttattttat tcgctattgc tgttactgaa ggaaaataga gagaatggaa
     3061 aattcacgca tccctgggga acattttttt accaccagtg ataatacagc gttgttttat
     3121 cggcactggc ccactttaca gccaggggcg aaaaaggtca tcgtcttatt tcatcgcggt
     3181 catgaacatt ctggtcgtct acaacatctc gttgatgaac tggcgatgcc agatactgct
     3241 ttttatgcat gggatgcccg agggcatgga caaacttcgg ggccgcgtgg ttatagtccg
     3301 tctcttgcga gttcagtgca agatgtcgat gaatttgtcc gttttgctgc cagcgacagc
     3361 caggtcggac tggaagaggt ggttgtgatc gcgcaaagcg tcggtgcagt gatggttgct
     3421 acatgggttc atgattatgc gcctgctatt cgcgggctgg tgctggcttc tccggccttt
     3481 aaggttaaat tgtatgtgcc gctggcacgt cctgcgctgg cgttatggca tcgtctgcgt
     3541 ggtctgtttt ttattaattc ctatgtgaaa ggacgctatt tgacccacga tcggcaacgg
     3601 gtggctagtt tcaataatga tccgctgatc acacgggcga ttgccgtcaa tatcttgcta
     3661 gatctctata aaacgtctga acgtattatt agcgatgcgg cggcgattac gctccccacg
     3721 caacttctga tatcaggcga tgactatgtg gtgcaccgcc aaccgcagat tgatttttat
     3781 cagcgtttac gtagccctct gaaagagctg catctgctgc caggctttta tcacgacaca
     3841 ttgggtgaag agaacagggc gcaggcattt gaaaaaatgc aaagctttat ctgccgctta
     3901 tatgctaaca aatcgcaaaa atttgattat cagtatgaag accgcacagg accatcagcg
     3961 gatcgatggc ggctcctttc tggtggagcc gtgccgttat cgccggttga tttggcgtat
     4021 cgctttatgc gtaaagcgat gaaattgttc ggggcgcact ctgcgggcct gcatctcgga
     4081 atgagcaccg gctttgattc aggcagttcg ctggattatg tctatcaaaa tcaaccgcaa
     4141 ggtagtaacg cattcgggcg tttagtcgac aaaatctacc tgaacagtgt tggctggcgc
     4201 ggtattcgcc agcgcaaaac ccatttacaa atgctgatta aacaagccgt tgccgatctc
     4261 cacgctaaag gtttagccgt ccgcgtggtt gacattgccg cagggcatgg acgctatgta
     4321 ctggatgcgc tggcgaacga gcctgccgta agcgatattt tcttacgtga ttacagtgag
     4381 ttaaatgttg cacaggggca agagatgatt gcccaacggg gaatgtctgg gcgggtgcgt
     4441 tttgaacagg gcgatgcgtt taacccggag gagctcagcg cgttaactcc gcggcctacg
     4501 ctggcgattg tctctggact gtatgagctt tttcccgaaa atgagcaggt aaaaaactca
     4561 ctcgcaggtc ttgccaatgc catcgatccg ggtggcattc tcatctacac cgggcagccg
     4621 tggcacccac aactggagct gattgccggg gtgttaacca gtcataaaga tggtaaaccg
     4681 tgggtaatgc gcgtgcgttc tcaaggggag atggattcgc tcgtgcatga tgccggattt
     4741 gataaatgca cacaacggat tgatgagtgg ggtattttta cggtttcgat ggcggtgcgt
     4801 cgtgataact gaacgtcgta acgtattgct acaaggcgct ggctggttat tgttgctggc
     4861 cccgtttttc ttcttcacct atggatctct taatcaattc accgcggttc aggaccttaa
     4921 cagccatgat atccccagtc aggtattcgg ttgggaaacg gcgatccctt tcattccctg
     4981 gactattgtt ccttactgga gtctggatct tttatatggg ttttcgttgt tcgtttgtag
     5041 ttcgacattc gaacagcgcc gactggttca ccggcttatt ctggcaacaa taatggcctg
     5101 ctgcggtttt ttcctttatc cgctgaagtt tagttttatc cgtcctgaag tgagtggggt
     5161 gacaggatgg ctattttcgc aacttgaact gtttgatctg ccttataacc agtctccttc
     5221 gctgcatatt attctctgct ggctactttg gcgtcacttt cgtcagcatc tggctgtgag
     5281 gtggcgtaaa gtctgcggcg gatggttttt actcatcgcc atttcgacgc tgacgacctg
     5341 gcagcatcat tttattgatg tcatcacagg gttggcggta ggtatgttga ttgactggat
     5401 gatgcccgtc gaccgtcgtt ggaattatca gaaacctgat caacgtcgaa tcaaaatagc
     5461 actgccatat gtcgtaggcg cgtgctcgtg cattgtattg atggagctaa tgataatgct
     5521 tcagttatgg tggtcagtct ggttatgttg gccggtatta tcgctactca ttattggccg
     5581 tgggtacggt gggcttggcg cgataacaac agggaaagat agtcagggga aactaccgcc
     5641 cgccgtttac tggctgacat tgccctgccg catcgggatg tggctgtcta tgcgttggtt
     5701 ttgccttcgc ctggagccgg taagcaaaat tacggcaggt gtttatttag gggcgtttcc
     5761 acgacatatt ccggcacaga atgcggttct ggacgtcacc tttgaattcc ctcgcggacg
     5821 agcgacaaaa gatcgactct atttttgtgt accgatgttg gatctggtgg ttccggaaga
     5881 gggggagctc cgacaggccg tggcgatgtt ggaaacatta cgcgaaaagc aaggcagcgt
     5941 tctggtccat tgcgcgttgg gattatcgcg cagtgcgctg gtggtggcgg catggctgtt
     6001 atgttacgga cattgtaaaa ccgttgatga agcgattagt taccttcgag ccagacgccc
     6061 gcagattgtg ctgacagacg agcacaaagc gatgctgaga ttatgggaaa acaggtaagt
     6121 ggattgagat gtggactgaa tatctacagt ccacatcaag accgtgtccg gttatgcagc
     6181 aacaatactg tcgatggctg ctttcgcgtc agactgcgct ttcgctgcca tttccggacc
     6241 gtatgcgatc ccttcggcga agacaaattt cacatcggta atgccgataa agccgaggaa
     6301 cgtggacaga tacggcgtca ccaggtccgt tggtccatct ttatggatcc caccgcggct
     6361 ggtgataacg atggcttttt taccggtgac caggccttcc ggaccgttct ccgtatagcg
     6421 gaaagtaacg cctgcgcgtg caaccaggtc aaaatagttt ttcaactgag tcgagatgtt
     6481 gaagttatac atcggtgccg caataacgat aacgtcgtgg gctttcaact cggcaatcag
     6541 ctcatcggaa agtgccagag cttcctgctg acgcggagtc agcgacgcat cgttcggacg
     6601 cagcgcacca accagttcgc catccagcac cggaatcgga tttgcagcca ggtcgcgaac
     6661 ggtgatttca tcagcggagt gcttttcgcg ccattgttca acaaaataat cggacaactg
     6721 attagactga gagtaccctg ccaggatgct ggatttaaga actaatacct tgctcatggt
     6781 gtttccttat agatgtttga atgggcgatg ccccgttgct tgttgacact ttattcacaa
     6841 tcctgccaca gagatagcgc aataaatcga agcctatgtt cgaatttatt gaacaacgca
     6901 tagaaagccg cgatgtggta ctctatatct atcatttaaa agaaaattaa tcaggcagcc
     6961 tactgcccac taacgttatg acagaacaac aaaaattgac ctttacggcc ttgcagcagc
     7021 ggctggattc gctgatgctg cgcgacagac tgcgtttttc tcgccgtctg cacggcgtga
     7081 agaaggttaa aaatcctgat gcacaacagg ccattttcca ggagatggcg aaagagattg
     7141 accatgcggc agggaaagtc ctgctgcgtg aagcggcacg accggaaatc acttatccgg
     7201 acaatttgcc ggttagtcag aaaaagcagg acatactcga agcgattcgt gatcaccagg
     7261 tggtgatcgt cgccggggaa accggttccg gtaaaacgac tcagctaccg aaaatctgta
     7321 tggaactggg gcgcgggatt aaagggctga tcggtcatac ccaaccgcgt cgcctggcgg
     7381 cacgaacggt ggcgaaccgt attgcggaag agctgaaaac ggagccgggc ggttgcatcg
     7441 gttacaaagt gcgtttcagt gatcacgtta gtgataacac gatggtcaag ctgatgaccg
     7501 acggtatcct gctggcggag atccagcaag accgcctgct gatgcagtac gacactatca
     7561 ttattgacga agcgcacgaa cgtagcctga atatcgattt tctgctcggc tatttgaaag
     7621 agttgctacc gcggcgtcct gacctaaaaa tcattatcac ttccgcgact atcgacccgg
     7681 aacgcttttc gcgccacttt aataatgcgc cgattatcga agtctccggt cggacctatc
     7741 cggtggaagt gcgctatcga ccgattgttg aagaagccga tgacaccgag cgcgatcagc
     7801 tgcaggcgat ttttgacgcc gtagacgaac tgagtcagga aagctccggc gacattctga
     7861 tctttatgag cggtgagcgg gaaattcgcg ataccgcaga tgcgctgaac aagctaaatc
     7921 tgcggcatac cgaaatcttg ccgctttatg cgcggctttc gaacagcgaa cagaaccgcg
     7981 tgttccagtc gcacagcgga cggcgcattg tgctggcgac caacgtcgcg gaaacgtcgc
     8041 tgaccgtacc ggggattaaa tacgttatcg acccaggtac ggcgcgtatc agccgctaca
     8101 gctatcgcac caaagtgcag cgtttgccga ttgagcccat ttcgcaggcg tctgccaacc
     8161 agcgtaaagg ccgctgtggt cgtgtgtccg aagggatctg tattcgtctc tattccgaag
     8221 acgatttcct ttcgcgcccg gaatttaccg atccggagat tctgcgtacc aacctggcct
     8281 cggttatttt acagatgacc gcgctggggt tgggcgatat cgccgcgttc ccatttgtcg
     8341 aagcaccgga taaacgcaat atccaggatg gcgtgcgtct gctcgaagaa ctgggcgcga
     8401 tcaccactga tgaacaggcc agcgcctata aactgacgcc gctcggtcgc cagctctcgc
     8461 agttgcctgt cgacccacgt ctggcgcgta tggtgctgga agcgcaaaaa catggctgcg
     8521 tgcgtgaggc gatgattatc acgtccgcgc tctccatcca ggatccgcgc gaacgaccga
     8581 tggacaaaca gcaggcatcg gacgaaaaac atcgccgctt ccacgacaaa gaatccgact
     8641 tcctcgcgtt tgtaaatctg tggaattatc tcggcgagca gcaaaaggcg ctttcttcta
     8701 acgccttccg tcgcctgtgt cgtaccgatt atctcaacta tctgcgcgtg cgcgaatggc
     8761 aggatatcta cacccagttg cgccaggtgg tgaaagaact tggcattccg gttaacagcg
     8821 aaccggcgga gtatcgcgaa attcacatcg ccttactgac cggtttgctt tcccatatcg
     8881 gcatgaaaga tgccgataaa caagaatata ccggcgcacg taacgcgcgt ttttccatct
     8941 ttcccggttc tggtttattc aaaaagccgc cgaaatgggt aatggtggcg gaactggtag
     9001 aaaccagccg cctgtggggg cgcattgctg cgcgtatcga ccctgaatgg gtagaacccg
     9061 ttgctcagca tttgattaaa cgcacctaca gcgaaccgca ctgggaacgg gcgcagggcg
     9121 cggtgatggc aacggaaaaa gtcactgttt atggtttgcc gattgttgct gcgcgtaagg
     9181 tcaactacag ccagatcgat ccggcgttat gtcgtgaact ctttattcgc cacgctctgg
     9241 tggaaggtga ctggcagacg cgtcacgcat tcttccgtga aaacctgaaa ctgcgggccg
     9301 aagtggaaga gctggaacac aaatcacgtc gccgcgatat tctggttgat gacgaaacgc
     9361 tgtttgagtt ctacgaccag cgcatcagcc acgatgtaat ctccgctcgt cacttcgaca
     9421 gctggtggaa aaaagtcagc cgcgaaacgc ctgatttgct caactttgaa aagagcatgt
     9481 tgatcaaaga aggggcggaa aaaatcagca agctggatta cccgaacttc tggcatcagg
     9541 gcaatctcaa gctgcgtttg agctatcagt ttgagccagg cgcggacgcg gacggtgtga
     9601 ccgtgcatat tccgctgccg ctgcttaatc aggtagaaga gagcgggttt gaatggcaaa
     9661 tccccggcct gcgtcgtgaa ctgatcatcg cactgattaa atcgttgccg aaaccggtac
     9721 gccgtaattt tgtgcctgca ccaaactacg ccgaagcgtt tttaggccgc gtcacaccgc
     9781 tggagttacc gttgctcgac agccttgagc gcgagttacg gcggatgacc ggtgttaccg
     9841 ttgaccgcga agactggcac tgggatcagg tgcccgatca cctgaaaatc accttccgcg
     9901 tggtggatga caaaaacaag aagctaaaag aagggcgctc tctacaagat ctgaaagatg
     9961 cgctgaaagg caaagtgcag gaaacgctgt ctgcggtggc ggatgacggt atcgagcaga
    10021 gcggcttaca tatctggagc tttgggcaac tgccggaaag ctacgaacag aagcgtggca
    10081 actacaaagt gaaggcgtgg ccagcgctag tggatgaacg cgacagcgtg gcgatcaaac
    10141 tgtttgataa tccgctggaa caaaagcagg caatgtggaa cggtcttcgc cgtctactgc
    10201 tactgaatat tccatcgccg atcaagtatt tgcatgaaaa gttaccgaac aaagccaagc
    10261 tggggctgta ctttaacccg tatggcaaag tgctggagct gatcgacgac tgtatctcct
    10321 gcggggtgga tcaactgatc gacgccaatg gtggcccggt ctggacggaa gaaggctttg
    10381 ctgcgctgca tgagaaagtc cgcgctgaac tgaacgatac ggtggtggat attgcgaagc
    10441 aggtcgagca aatccttacg gcagtgttca atatcaacaa acgcctgaaa gggcgggtgg
    10501 atatgactat ggcgctgggg ctttctgaca tcaaagcgca gatgggcggg ctggtgtatc
    10561 gcggttttgt gactggtaac ggcttcaaac ggttgggcga cacgctgcgt tatttgcagg
    10621 cgattgaaaa acggctggaa aaactggcgg ttgacccaca tcgcgaccgt gctcagatgc
    10681 tgaaagtcga aaatgtccag caggcgtggc agcaatggat caacaaactg ccgcccgcac
    10741 gtcgtgagga tgaagacgtg aaagagatcc gttggatgat agaagagttg cgcgttagtt
    10801 acttcgctca acaacttggt acgccttatc cgatttcaga taagcgtatt ttgcaggcga
    10861 tggagcagat tagcggttaa cactgctatt tgcctgataa atctaaaacc cggtaagcat
    10921 ttagcgccgg gttttttatt aattctaaaa cggtaagggc aaaaattcag gaattcagaa
    10981 aaatacaatt ctcagctgca agatgaataa tgtttatcta cagcatttcc ttaaaagata
    11041 tgtcaggctt gcggagtggc ggttaaggaa ataggacttc ctcctttcag agtgctccgc
    11101 ttctcactat tatctcacgc agtattctta agggaacgat aaggaggaac catgaacatt
    11161 accccgtttc cgacgctttc gccggcaacc atagatgcca taaatgttat cggtcagtgg
    11221 ctggcgcagg atgatttctc cggtgagatg acgtatcagg ccgattgcgt gatccttgca
    11281 ggcaatgcgg ttatgccgac tatcgacgcg gcatgtaaga ttgcccgcga tcagcaaatt
    11341 cctttactga tcagtggtgg tatcggtcac tcgacaactt ttttgtatag cgccatcgca
    11401 cagcatccgc actacaacac tatccgcacc actggcagag cagaagcgac catcctggcg
    11461 gatatcgctc atcagttctg gcacattccg catgaaaaaa tctggattga agaccagtca
    11521 acaaactgcg gtgaaaacgc acgctttagc atcgcgctat tgaatcaggc cgtagaacga
    11581 gttcatacgg ctatcgttgt tcaggacccc accatgcagc ggcgcacgat ggcgacgttc
    11641 cgccgtatga ctggggacaa tcccgatgca ccacgctggt taagttatcc cggattcgtt
    11701 cctcagttag gaaataacgc agacagtgta atctttgtta atccgttgca aggattatgg
    11761 ccggttgagc gttatctctc actactcact ggcgagctgc cacgtttacg cgatgatagc
    11821 gatggctacg gtccacgcgg gcgagatttt atcgttcacg ttgattttcc ggcagaagtc
    11881 atccaagcat ggcaaacgct gaaacacgat gcggtgctca tcgaggcgat ggaaagtcgc
    11941 tcgttacgtt aaaaattgcc cgtttgtgaa ccacttgttt gcaaacgggc ataactcctg
    12001 ccttttattt ctgcctctta ttccttttat acttgttttt atgaagccct tcacagaatt
    12061 gtcctttcat gatgccgtct ctatgatgat tgatgttaat taacaatgca tttaccaaaa
    12121 acaaacatat aaatcacagg agtcgctcat gtcagtaccc gttcaacatc ctatgtatat
    12181 tgatggacag tttgttacct ggcgtggaga cgcatggatt gatgtggtaa accctgctac
    12241 agaggctgtc atttcccgca ttcccgatgg tcaggccgag gatgcccgta aggcaatcga
    12301 tgcagcagaa cgtgcacaac cagaatggga agcgttgcct gctattgaac gcgccagttg
    12361 gttgcgcaaa atctccgccg ggatccgcga acgcgccagt gaaatcagta cgctgattgt
    12421 tgaagaaggg ggtaagatcc agcagctggc tgaagtcgaa gtggctttta ctgcggacta
    12481 tatcgattac atggcggagt gggcacggcg ttatgaaggc gagattattc aaagcgatcg
    12541 tccaggagaa aatattcttc tgtttaaacg tgcgcttggc gtgactaccg gcattctgcc
    12601 gtggaacttc ccgttcttcc tcattgcccg caaaatggct cccgctcttt tgaccggtaa
    12661 taccatcgtc attaaaccca gtgaatttac gccaaacaat gcgattgcat tcgccaaaat
    12721 tgtcgatgaa ataggccttc cgcgcggcgt gtttaacctt gttctggggc gtggtgaaac
    12781 cgttgggcaa gaactggcgg gtaacccaaa ggtcgcaatg gtcagtatga caggcagcgt
    12841 ctctgcaggt gaaaagatta tggcgactgc ggcgaaaaac atcaccaaag tgtgcctgga
    12901 attggggggt aaagcaccgg ctatcgtaat ggacgatgcc gatcttgaac tggcagtcaa
    12961 agccatcgtt gattcacgcg tcattaatag tgggcaagtg tgtaactgtg cagaacgtgt
    13021 ttatgtacag aaaggcattt atgatcagtt tgtcaatcgg ctgggtgaag cgatgcaggc
    13081 ggttcaattt ggtaaccccg ctgaacgcaa cgacattgcg atggggccgt tgattaacgc
    13141 cgcggcgctg gaaagggtcg agcaaaaagt ggcgcgcgca gtagaagaag gggcgagagt
    13201 ggcgttgggt ggcaaagcgg tagaggggaa aggatattat tatccgccga cattgctgct
    13261 ggatgttcgc caggaaatgt cgattatgca tgaggaaacc tttggcccgg ttctgccggt
    13321 ggtcgcattt gacacgctgg aagaggctat ctcaatggcg aatgatagtg attacggcct
    13381 gacctcatca atctataccc aaaatctgaa cgtcgcgatg aaagccatta aagggctgaa
    13441 gtttggtgaa acttacatca accgtgaaaa cttcgaagct atgcaaggat tccacgccgg
    13501 atggcgtaaa tctggtattg gcggcgcaga tggcaaacat ggcttgcatg aatatctgca
    13561 gacccaggtg gtttatttac agtcttaatg agtggaagag gcggaggttt ttcctccgcc
    13621 tgtgtgcgtc agagtttagc gaatttttcg agggtgcgaa tgagttgtgt gacgaagcca
    13681 tattcgttat cgtaccaggc gaccgttttc accagttgta aatcgcccac ggcggtaatt
    13741 tccgtttgcg tggcatcaaa caccgacccg aaatggctgc caatgatatc ggaagagact
    13801 atttcttcat cggtataacc aaatgactcg ttattggtgg ttgcttgttt aagtgcgtta
    13861 ttcacctctt cggcagtcac tttttttgcg agaatggaca ccagctcagt gaccgaacct
    13921 gttttcaccg gcacgcgttg cgcatgacct ttcagtttgc cgctcagttc cgggatcacc
    13981 agaccaatgg cttttgccgc acctgtagtg tggggaatga tattttctgc cgctgcgcgt
    14041 gaagctcgta aatccttccc acgcggacca tccaccagtg actgggtgcc ggtataggca
    14101 tgaatggtcg tcatcgtgcc gacttctatc ccgaaactgt catgcaaggc tttggccatc
    14161 ggcgcaaggc agttagtggt gcatgacgcc acggaaacaa tggtgtcgtt gccatccaga
    14221 gtgtcgtcat tgacgttata aacgatagtt ttcatttcac cggcaggggc ggaaatcaac
    14281 accttcttcg caccagcatc aaggtgcgcc tgcgatttct ctgcggaggt ataaaagcca
    14341 gtacattcga caatgatttc tgcgcctttc gctttccatg gaatattttt agcctctttt
    14401 tcggcgtaaa ccgcgatact ttttccatca acgataagtg aatcttccgt aaaatcaacg
    14461 ctccagggga acggtccgta gtttgaatca tgtttcagca ggtaggtgag aatttttggg
    14521 gaagtgagat cattaatagc gacaacgtct atgttgcttt tgacttcaag taatcgacgc
    14581 aacaccagtc gaccgatacg accaaaacca ttaataccaa ctttactcat ggtcttctcc
    14641 tgtcaggaac gttcggatga aaattgatcc tttccaggct tagaccagga tggcgggatg
    14701 ggcaatctac attcttgcag tgaaacgtaa cgtactgaaa acgggtgaac aatatttaat
    14761 gaaattttaa gaaaagcccg ttatgttaac ggcaaattat gttaaagcag gaaatgttat
    14821 gggaaataaa tactcaaggt tacaaatcag cattcactgg ctggtctttt tgctggttat
    14881 cgtagcgtat tgcgcaatgg agtttcgtgg tttcttccca cgtagcgatc ggcccctcat
    14941 caacatgatt catgtttcct gtggcatctc aattctcgta ctgatggtgg ttcgtctttt
    15001 gttaagactg aaatacccaa ccccgccgat tatacctaag ccaaaaccga tgatgacggg
    15061 actggcgcat ttgggccatt tggtgattta tctcctgttt attgcgctgc cagtgattgg
    15121 tttggtgatg atgtataacc ggggtaaccc gtggtttgcg tttggtatga cgatgcctta
    15181 tgcatcagag gccaatttcg aacgggttga tagcttaaag tcgtggcatg aaacgctggc
    15241 gaatctggga tattttgtca tcgggttgca cgctgcggct gcactggcac accactattt
    15301 ctggaaggac aacacacttc tacgcatgat gccgcgtaaa cgttcctgaa ggatatttaa
    15361 agaaaacgcc tgtactaaaa ccgacccgtg gtacaggcga agaatacggg tctacatcgg
    15421 aagcgcctat tatatttatt tgtatgacaa ataaaaccat aatccttgcc catacgtcca
    15481 tctggcttat ttttaatcaa ttcacccgat ctttgatctc atcaacggta tcaaaataaa
    15541 ccagcgtata acgttaaaat cgaacataag attcgataaa tcatggaggt tatatgaaaa
    15601 aactggcact tatcttggtt ataggaacgc ttgtttcctt ttatgccgat gccgggcgca
    15661 aaccctgttc tggttcgaaa ggggggatct cacactgtac ggcaggcggc aaatttgttt
    15721 gtaatgatgg ttctattagt gcatcgaaaa aaacatgcat taactgaagt gtaaaagggg
    15781 tgccatgaga aaatgaatct gctagtcaga tgcgcgggga aaatccccgc gcttgccctt
    15841 acctggacgt gcaggccatg agcgcagcaa cctccttatc accgtcccgg aaccgcagtt
    15901 cgtagagcgt ttgtcgggtc aggagtgtga atgtcagaat cgtaatgcag ataatgagca
    15961 gacacaccac cagagggttg cgcttcatag cctttctcct tgccggatgg tgggtaagag
    16021 gctaagatct gaattgctgt gttcattcgt tggcctcggt tgatagaaat atcggtcggg
    16081 gccttcgtct ttctgattcc cggttagcct gaaaacagaa agtctcaggc acccgcaggc
    16141 atcctatgag gtttccttag ggacgaaaat aatcacttta cgaaattgcg tgctgttttc
    16201 cagaattttt cgtcattcgg gttagccagt ttagccattc gttactctct tcattccaat
    16261 agcattaatt ttctctgcaa taattgttgt aaaaatgtga cgcaaagagg tttttggtca
    16321 taagtaatta ccgtcaagtg ccgatgactt tctatcagga gtaaacctgg acgagagaca
    16381 acggtaatga atacaactcc ctcacagcga ttaggttttt tgcatcacat caggctggtt
    16441 ccgttatttg cctgcattct tggcggtatc ttagttctat tcgcattaag ttctgctctg
    16501 gctggctatt tcctctggca ggccgatcgc gatcagcgtg atgttactgc ggagattgag
    16561 attcggaccg ggttagcgaa cagttcagat tttttgcgtt cagcccggat caatatgatt
    16621 caggccgggg cagcgagtcg tattgcggaa atggaatcaa tgaagcgaaa tattgcgcaa
    16681 gccgaatcgg agattaaaca gtcgcagcaa ggttatcgtg cttatcagaa tcggtcggtg
    16741 aaaacacctg ctgatgaagc cctcgacact gaattaaatc aacgcttcca ggcttatatc
    16801 acgggtatgc aacctatgtt gaaatatgcc aaaaatggca tgtttgaagc gattatcaat
    16861 catgaaagtg agcagatccg accgctggat aatgcttata ccgatatttt gagcaaagcc
    16921 gttaagatac ttagcaccag agccaaccaa ctggcggaac tggcccatca gcgcacccgc
    16981 ctgggtggga tgttcatgat tggcgcgttt gtgcttgccc tggtcatgac gctgataaca
    17041 tttatggtgc tacgtcggat cgtcattcgt ccactgcaac atgtcgcaca acggattgaa
    17101 aaaatcgcca gtggcgatct gacgatgaat gatgaaccgg cgggtcgtaa tgaaatcggt
    17161 cgcttaagtc gtcatttaca gcaaatgcag cattcactgg ggatgacagt agggactgtt
    17221 cgacagggtg cggaagagat ttatcgtggc accagcgaaa tttccgctgg caatgcggac
    17281 ctttcatctc gcaccgaaga acaagcggcg gcaatcgaac aaactgccgc cagcatggag
    17341 caactcactg cgacggtgaa acagaatgcg gataacgcgc atcatgccag caaactggcg
    17401 caagaggctt ctattaaagc cagcgatggc gggcagacgg tttccggtgt agtaaaaacg
    17461 atgggtgcta tctccacgag ttcgaagaaa atttctgaga tcaccgccgt catcaacagt
    17521 attgctttcc agacgaatat tctggcactg aatgctgccg ttgaagccgc gcgagcgggt
    17581 gagcaagggc gtggatttgc cgttgtcgcc agcgaagtac ggacactcgc aagccgcagc
    17641 gctcaggcgg cgaaagagat tgaaggcttg atcagtgaat cagtcaggtt aattgacctg
    17701 ggatcggatg aggtggccac ggccgggaaa accatgagca ctattgttga tgccgtcgcg
    17761 agtgtcacac atatcatgca ggaaattgcc gccgcctcgg atgagcaaag taggggtata
    17821 acgcaggtta gccaggcgat atctgaaatg gataaggtga cgcaacagaa tgcttctctg
    17881 gtagaagagg cctcagcagc ggcagtgtcc cttgaagaac aggcggcacg attaactgag
    17941 gcggtggacg tattccgtct gaacaaacat tctgtgtcgg cagaacctcg cggagctggt
    18001 gaaccagtta gtttcgctac agtgtgaaaa tgttaaagga gggatcgaca gatcccttca
    18061 cctttcagaa cggcattgat tttcgaatag cgttaatcat caactggcaa ccagaagaga
    18121 acgtcgcatc tacgcgggtc agtattccaa tcggttcgcc tgcaccatga cccggaacag
    18181 gcagggccac cagcgtggca tgacgcaggt cgtcttttac agcgccagaa gggacaaacc
    18241 acacgtaatc gtattcaacc gtaagttgac gagatagcga agcagacagc gtttcgatac
    18301 aacccgaagg aattttacat ccctggctct gcactaatgc atctgaatgc tggcgtggcg
    18361 cagtgccttc tggtgataca acgaccggcc attccagcac ccggcttagc gttacgttct
    18421 cctgaagtag cggatgatca gggcggacaa ccagcttcag cgattcaaga aacagcagtt
    18481 cgtaattaag tccggtcatc agttcaggat ctgacatccg accaatgccg atatcgattt
    18541 ccccggtttt caaacccgcc agaatcatag ggttactcat tgtcgcaact tgcaaggtcg
    18601 tttctttttg ttgctgatga aactggccta taaccgaagg taatatcccc agtgccgcag
    18661 taggtagtgc accaaccctg acgacatcat tattaagacc ttctttacga tgaagcgact
    18721 gtccggcagt gttgatggcg tcaagaactc tgactgcatg cgttaaaaat tgttcgccgg
    18781 gtaaggtaag ttgcgccccc tgacgaccac gctcaaacaa gcgagcgccc gtcagctgct
    18841 ccagttcatt caatgtctta gagagcgcag gttgactcaa attaagggtt tcagccgcgc
    18901 gccccaaagt tccttgttgt gcgacagcta cgaatgtatg aaggtggcgc aaacggatgc
    18961 gctgactaaa cagactattt ttttccataa gcgatgttaa aaacgaagcg gtgtcgctga
    19021 caagtgaagt tgtttgatta tgataac
//
LOCUS       DAPHCV010000096        18687 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.096, whole genome shotgun sequence.
ACCESSION   DAPHCV010000096 DAPHCV010000000
VERSION     DAPHCV010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.096"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..919
                     /locus_tag="RH033_003468"
     CDS             <1..919
                     /locus_tag="RH033_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HDV2992600.1"
                     /translation="IIFYQPWVRALPPTPRHASPEQLEKTVRYLTQTVHPRSADNIDN
                     LNRSAEYIKEVFISSGARVTAQDVPITGGPYKNIVADYGPADGPLIIIGAHYDSVSSY
                     ENDQLTYTPGADDNASGVAGLLELARLLQLQTPGIGVQLVAYASEEPPFFRSDEMGSA
                     VHAASLERPVKLMIALEMIGYYDSTPGSQDYPSPAMSWLYPDRGDFIAVVGRMQDINA
                     VRQVKAALLSSRDLSVYSMNAPGFIPGIDFSDHLNYWQHDIPAVMITDTAFYRNKQYH
                     LPGDIADRLNYQKMAQVVDGVTTLLYNSK"
     gene            1022..1525
                     /locus_tag="RH033_003469"
     CDS             1022..1525
                     /locus_tag="RH033_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HDV2992601.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTKKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLEA
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1592..3049)
                     /gene="nuoN"
                     /locus_tag="RH033_003470"
     CDS             complement(1592..3049)
                     /gene="nuoN"
                     /locus_tag="RH033_003470"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HDV2992602.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3056..4585)
                     /gene="nuoM"
                     /locus_tag="RH033_003471"
     CDS             complement(3056..4585)
                     /gene="nuoM"
                     /locus_tag="RH033_003471"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HDV2992603.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4816..6657)
                     /gene="nuoL"
                     /locus_tag="RH033_003472"
     CDS             complement(4816..6657)
                     /gene="nuoL"
                     /locus_tag="RH033_003472"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HDV2992604.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVFSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6654..6956)
                     /gene="nuoK"
                     /locus_tag="RH033_003473"
     CDS             complement(6654..6956)
                     /gene="nuoK"
                     /locus_tag="RH033_003473"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HDV2992605.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6953..7507)
                     /gene="nuoJ"
                     /locus_tag="RH033_003474"
     CDS             complement(6953..7507)
                     /gene="nuoJ"
                     /locus_tag="RH033_003474"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HDV2992606.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7519..8061)
                     /gene="nuoI"
                     /locus_tag="RH033_003475"
     CDS             complement(7519..8061)
                     /gene="nuoI"
                     /locus_tag="RH033_003475"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HDV2992607.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8076..9053)
                     /gene="nuoH"
                     /locus_tag="RH033_003476"
     CDS             complement(8076..9053)
                     /gene="nuoH"
                     /locus_tag="RH033_003476"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HDV2992608.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9050..11776)
                     /gene="nuoG"
                     /locus_tag="RH033_003477"
     CDS             complement(9050..11776)
                     /gene="nuoG"
                     /locus_tag="RH033_003477"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HDV2992609.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSAE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11829..13166)
                     /gene="nuoF"
                     /locus_tag="RH033_003478"
     CDS             complement(11829..13166)
                     /gene="nuoF"
                     /locus_tag="RH033_003478"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HDV2992610.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13163..13663)
                     /gene="nuoE"
                     /locus_tag="RH033_003479"
     CDS             complement(13163..13663)
                     /gene="nuoE"
                     /locus_tag="RH033_003479"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HDV2992611.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13666..15468)
                     /gene="nuoC"
                     /locus_tag="RH033_003480"
     CDS             complement(13666..15468)
                     /gene="nuoC"
                     /locus_tag="RH033_003480"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HDV2992612.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15562..16224)
                     /gene="nuoB"
                     /locus_tag="RH033_003481"
     CDS             complement(15562..16224)
                     /gene="nuoB"
                     /locus_tag="RH033_003481"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HDV2992613.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16240..16683)
                     /gene="nuoA"
                     /locus_tag="RH033_003482"
     CDS             complement(16240..16683)
                     /gene="nuoA"
                     /locus_tag="RH033_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HDV2992614.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17313..18251)
                     /gene="lrhA"
                     /locus_tag="RH033_003483"
     CDS             complement(17313..18251)
                     /gene="lrhA"
                     /locus_tag="RH033_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HDV2992615.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPST
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4648 a         5193 c         4828 g         4018 t
ORIGIN      
        1 tattattttt taccaaccct gggtcagggc attaccacca acgccccgac acgctagtcc
       61 tgagcaatta gaaaaaacag ttcgttatct tacacaaact gtgcatccac gtagcgccga
      121 caatattgat aatctgaata ggtccgcaga gtatataaaa gaggtcttta tcagtagcgg
      181 tgccagagtt accgcgcagg atgtccccat tacgggtggc ccctacaaaa acattgttgc
      241 tgattatggt cctgccgatg gaccgctgat tattattggt gcgcactatg actctgtcag
      301 tagttatgaa aacgatcaat tgacctatac gccgggcgcg gatgataacg ccagcggcgt
      361 ggcaggatta ctcgaactgg cgcgtttgtt acagctgcag actccaggga ttggcgtcca
      421 actggtcgcc tatgcgtcgg aagaaccgcc cttctttcgt agcgatgaaa tggggagcgc
      481 ggtacatgca gcttcgcttg agcgtccagt gaaattaatg atagcgctgg agatgattgg
      541 ttattacgac tcaacgcctg gtagccagga ttacccatcc ccggcaatgt cctggcttta
      601 tcccgatcgg ggagacttta ttgccgtggt cggcagaatg caggatatca acgccgttcg
      661 tcaggtaaaa gcggcattgt tgtcatcccg ggatttatct gtttattcta tgaatgcccc
      721 aggttttatt cccggtattg atttctctga tcaccttaat tattggcaac acgatattcc
      781 cgccgtaatg attactgaca ccgcttttta tcgtaataaa caataccact tgcccggtga
      841 tatcgcagac agattaaatt atcagaaaat ggctcaggta gtagatggag ttacaacctt
      901 gttatacaac agtaaataat aaaaatttct cgaggcggat ccagaaaaag caacatgcgc
      961 attttatttt ctttcgtctg ttttaagatg cgaaacatga aattcaacgg aggaaaaata
     1021 aatgggaatg attggctatt ttgcagaaat tgactcagaa aagattaacc aactgcttga
     1081 gtctactaaa aaacctttga tggataatat acatgacacg ctttcaggat tacgccgact
     1141 ggatattgat aaacgctggg attttttaca ctttggcctg acgggtacat ctgcatttga
     1201 ccccgcgaag aatgacccgc taagtcgcgc agtgcttggt gaacacagcc tagaagatgg
     1261 catagatggc ttcctgggat tgacctggaa tcaggagtta gccgcgacaa tcgaccgact
     1321 ggaagctctg gatcgaagtg agctgcgtaa acagttttcc attaagcgac tcaatgaaat
     1381 ggagatttat ccgggagtaa ccttttcaga agagttagag gggcaactct ttgcttcgat
     1441 catgcttgat atggaaaagc tgattagcgc gtatcgacgt atgctgcgac aagggaatca
     1501 tgcgctgacg gttattgttg ggtgatggtg tcagggcacc aaaaaaaacc gccgaacatg
     1561 tcggcggctt tctgacttac aaactgactg cttacatcag cggcattgct aaccgcacaa
     1621 tgctaatcag cggttgtggc catacaccca gcaccagtac caacagtgca gagatcagca
     1681 ccacgatacc gcctgcgctg tactgccagt ttgatggtgc atcgcgaccc ggttgttccg
     1741 gagcgtgaag gtataagctc accgccacgc gcaggtagta gtagaggccg attgccgaac
     1801 cgacaactac ggcacccacc agccaccaca agtgtgcctg gacaccgacc gccagcacgt
     1861 agaacttacc gataaagccc agcgtcattg ggataccagc cagtgaaagc atcatcaccg
     1921 tcatcactgc cgcgaggatc ggacgatgcc agaacagacc gcggtaagag aacagggaat
     1981 cagcatccgg gccacgatac gggctggaca tcaggctgac cacgccgaac gcgccgaggc
     2041 tgctgaacag ataacctgcc aggtaaaccc ctaccgcttc catcgacatc tcgccagttt
     2101 gcagcgcaat cagcgccacc agcagatagc cgaggtgaga gatagatgag taaccgagca
     2161 gacgtttgat attggtctgg ctcagcgcca tcaggttacc gaagatgatg gaggcaaagg
     2221 cgataatcgc cagcaccacg cgaatcgctt cgctgtcacc caccggtgcg tagaggaaca
     2281 gacgcatcac cacaccgaag atagcgattt tgctcgccgt cgccaggaaa gtggaaaccg
     2341 gcgcaggcgc gccctggtat acgtctggcg tccacaggtg gaacggcacc agagagagtt
     2401 tgaagccgag gccgacaatc atcaggccga aacctgccag caacagcggc tcgttgagca
     2461 taccgtcgcc gaggttttta cccagcgcga caaacgacag gtcgccagac tgcgcataca
     2521 ccagcgccat accgaacagc aggaaagaag acgctgcggc agagagaatg gtgtatttga
     2581 tactggcttc cagcgaacgt ttctggcgga aagcgtaacc gaccaggcca aacagcggca
     2641 aagagatcag ttcgataccg aggaacagag acgccagatg gttggcattc gccagcagga
     2701 tcccgcccag ggcggcaatt aacaccaaca ggtagaactc gtccttgttg tcgttatagc
     2761 cttcaagcca cgggtaggcg aaagtacagg tggcgaggct cgccaacaat accagcccgg
     2821 tgtaaagcat ggcgaaacca tcaacgcgca tcagtggcgt aacgtccata gcgcccgcct
     2881 ggccgacaaa ccagagcgaa accagcgccg cgttaagccc aataaccgag agcgtagcgt
     2941 tgaggaaatg attgcgtcgc cacgcaatgg agagcatcac caccaccacc gtcaagccga
     3001 cgatcagcaa cggtagcagt gcgatcaggt tttgtggagt tattgtcatg gcgatttacg
     3061 gccttgtagt agtaacggaa ttaacaaacc actgctggat attgccaatc gcggagtgcg
     3121 aggtatccag aatcggctgc ggatagaagc ccagcagtac cagcagcacc accagcaaca
     3181 ggatcataaa cagctcacgc agcgacatcc ctggcagttc ctggctggca atctggcttt
     3241 tcgctttacc gaagtaagcg cgatgtaaca tcgccagcga ataaacagat gcaaagacca
     3301 gcccaaaggt agagataacg gtaatcaccg ggacaacctg gaagctgccg aacagaatca
     3361 taaattcgcc gacgaagtta ccggtgccag gcatcccaag cgttgccacc gcaaagaaca
     3421 gcgacagtgc tggcagccat ttcatcttgc tccacagacc gcccatcatg cgcatgtcgc
     3481 gggtatggat acgttcataa agctgaccgc agagaataaa cagacccgcc gccgacaagc
     3541 cgtgcgcaat catctggatt accgcgccct ggtaggccaa ctggctgcca gtgtagatag
     3601 caatcagcac gaagcccatg tgggaaaccg aggtgtaggc gatcagacgt ttgatatcgg
     3661 tctgggcgaa ggccatccac gcaccgtaga agatgccgat aacacccagc cacatagcga
     3721 ttggcgcgaa ctctgccgac gcgttcggga acaacggtag ggagaaacgc agcagaccgt
     3781 aagcggcggt tttcagcaag atccccgcga ggtcaacgga accggcggtt ggagcctgtg
     3841 agtgcgcatc cggcagccag ccatgcagcg gaaccaccgg cattttcacc gcaaatgcga
     3901 tgaagaagcc cagcatcaac agatattcca caccactgga cattggcgta ttcaacagct
     3961 cttcatagtt gaaggtccag acgccggtcg cattgtagtg aacaaaaacc agcgccagga
     4021 tggcgatcaa catcaccaga ccactcgcct gggtgtaaat gaagaacttg gttgccgccg
     4081 tgatacgcgt tttaccgtca gaggctttat gcccccacag tgcgatcagg aagtacatcg
     4141 gcaccagcat catttcccag aagaagaaga acaggaacat gtcgatggca aggaacacgc
     4201 cgataacgcc gcccaggatc cacatcaggt tgaggtggaa gaagccctga tatttttcga
     4261 tctctttcca cgaacagagt accgccagca caccgagcag accggtcagc acgaccatca
     4321 gcagcgacag cccgtcaatg gcgagatgga tagagatacc aaaacgcggg atccacggca
     4381 tgtcgaattc agactgccac tgcggaattc cggcggattg cgtcagtgaa taaccgccct
     4441 gcaaccacag ttgcagcgac agcgccagcg tcaatcccat ggtgatcagc gcgatccagc
     4501 gcggcacctt gacgccaaag cgttcggtct gccagcacag gaagccgccg ataaagggga
     4561 ttagtattaa ccagggtaat aacatagcta agtatgtccc ttatatttac tcctgctcag
     4621 gctatctcac tcgccagccc gaatcccggc agtgctcgca atcctcacgt acttaagtac
     4681 gctccggttg ctgtgcgctg gcggtatccg gtctgacttc gccgatgacg cctgtacttc
     4741 aggaacagtt acctaaaatt tattcttagt tcacagaccc ggcgcaacgc cgggtatatt
     4801 cactcagcca acaactcaac gcagtaccat caacagtgcc agcaccacga ccgcaccgat
     4861 gctcatggat gccacatacc agcgcagata accgttctcg cttaacagca gacctttacc
     4921 tgcaaagcgg gaaaagacag ccgggatgtt catcattgag ttcagcggat cgcgtttcag
     4981 caaccaggca atacccagga acggcttgac gaacactttg tcgtacagcc agtcaaagcc
     5041 ccaggcgttg taccaccagg tgcccagcag acggcccggc gcactgttgg cgatggaggt
     5101 caccagagta cgtttaccca gccacagcca ggctgccagc agaatgccga ccaccgcgac
     5161 tacgccagag gtaatttcca gggtcaacat gctgccgtgc gccagttccg tcgtttgcgg
     5221 aagcacgccc tgcagcggcg gtacaatcag tgcgccaacg aaggtggaaa ggatcagcag
     5281 cacaatcagc ggcaggctgt gagttacccc tttcacggcg tgagcgtgaa tttgttcttt
     5341 tccgtggaag acgatgaaaa tcatacggaa ggtgtagagc gaggtcataa acgcaccgac
     5401 cagacctgcc accatcagat tgatatgacc attcgccatc gcacccgcga ggatctcatc
     5461 cttactgaag aagcccgcag tgaccagcgg cagtgccgac agtgctgcgc cgcccaccag
     5521 gaagcagaga taaaccagcg gaatagattt acgcagaccg cccatcttga agatgttctg
     5581 ttcgtgatgg caggccagaa tgacggaacc ggatgccagg aacagcagcg ctttaaagaa
     5641 cgcgtgggtc atcaagtgga aaatcgccgc atcccatgcc tgcacgccaa gcgcgaggaa
     5701 catgtagcca atctggctca tggtagagta agcgagaaca cgtttgatat cggtctgtac
     5761 cagcgcggca aaaccggcca gcagcagcgt aaccgccccg acaatgccca ccagatgcag
     5821 aacttccggc gtcatcagga acaggccgtg ggtacgagcg atcaggtaga cacccgcggt
     5881 taccatcgtt gcggcgtgga tcagcgcgga gacaggcgtc gggcccgcca tcgcgtcggc
     5941 aagccatgtc tgcaacggca actgcgcaga tttaccgacc gcaccgccca gcagcatcag
     6001 cgtcgcccac atcagcatgt tattgccgtc agcaaagtgc gctggtgcca gttccaccat
     6061 ttcgcggaag ttcagggtgc ccagttcgtt gtaaagaatg aacagcgcga aagcgaggaa
     6121 cacgtcaccc acacgggtca cgacgaacgc tttcattgcc gctgcgccat tcttcggatc
     6181 ggtgtaatag aaaccgatca gcagatagga gcacaggccc acgccttccc agccgaggta
     6241 catcaacagt aggttatcgg cgagaaccag aactaccatg ctggcgatga acaggttggt
     6301 gtaagcgaag aagcgagagt agccctcttc tccgcgcata taccaggagg catacatgtg
     6361 aataaggaaa cccacaccag tgaccactga gagcatggtc agcgacaggc cgtccagcac
     6421 cagattgaaa ccgatgttaa agtcgcctac cgacatccac gtccacagcg gctggctgta
     6481 tgcctgctca ccgttagcga agaagtcaac gccgataaag gcggttacca gcgccgccag
     6541 gcccacagag ccaacgccga cgatcgccga gacgttttca gaccagcgtc cacgggagaa
     6601 cgccagcagg acgaagccaa tcaatggcaa aataatggtt aaggcaagca tgttcatccg
     6661 cgcatctcac ttactgaatc gatgttcagg ttctggcgac gacggtgaag ttgcagcagc
     6721 agcgcaaggc cgatactcgc ttccgccgcc gcgaggctga tggcgagaat gtacatcacc
     6781 tgaccgtcgg tctggcccca gtagcttccg gcgaccacaa aggccagcgc agaggcgtta
     6841 atcatgattt ccagaccaat cagcataaac agcagattgc gacggataac cagaccggtt
     6901 aagccaagaa cgaataagat tgccgcgagg atcagtccat gttgtaaggg gatcatgcgt
     6961 gctcctccgt ttttcttttc gcgctgtcgt ctttacgatt gctcagcact tcacccgcac
     7021 gctcttcacg accaacgtgg aaggccacaa ccagaccagc cagcagcagc atagaagcca
     7081 gttccaccgc cagtacgtaa ggcccgaaca gcgtaatacc cactgcttta gcactgattg
     7141 gcgtaccgtc gataccctga tcattaacac caaggatggc gtaaacaatc accaccagca
     7201 tgatggccga caaaatcgcc ggaccaatcc acacctgcgg tttcagccac tggcgttcct
     7261 gttcgatttc tgaaccgccc aggttgagca tcatcaccac gaacacgaac agcaccataa
     7321 tggcacccgc gtagacgata atttccagcg caccggcgaa gtaagcgccc agtgagaaga
     7381 acaccccaga aatcgccagc agcgaaataa tcaggtacag cagcgcgtgt accggattgg
     7441 tatgggtgat cactcgcaag gtcgcaagta tggctatcag gccacagata taaaaagcga
     7501 actccattgc ccctctcctt acggtaacag gctcttgacg tcgataggct tggcttcgtt
     7561 ctctgcttcg cccttatctt tgccgtcgat tgccatacct gccatccggt agaagttata
     7621 ttccgggtat ttgcccggac cggagatcag cagatcctct ttctcgtaaa ccagatcctg
     7681 gcgcttgtat tcccccattt cgaaatccgg ggttaactga atcgccgtgg tcggacaggc
     7741 ttcttcgcac agaccacaga aaatgcagcg tgagaagttg atgcggaaaa attccggata
     7801 ccagcgaccg tctttggttt ctgctttttg cagcgagata cagccgaccg ggcaggctac
     7861 cgcgcagagg ttacaggcta cgcaacgctc ttcgccgtcc ggatcgcggg tcagaacgat
     7921 acgaccacga taacggggcg gcagatagac cggctcttcc gggtacattc gcgtttcgcg
     7981 tttggcgaac gcgtgcaggc cgatcatcca gatactacga acctgggtgc cgaaacctac
     8041 taacaattct tttaaggtca tggtcttatt gccccttatt gcgcctgcca gagaatgaca
     8101 gccgccgtta ccagcaagtt gatcagcgtc agcggcaggc agatcttcca gccgaaggac
     8161 attacctggt cataacgcgg acgcggtaac gacgcacgaa tcaaaatgaa catcatcata
     8221 aagaacgcgg ttttcagcgc gaaccagatg aatggcggta acaacgggcc ttgccagcca
     8281 ccgaagaaca gcgtcaccat cagtgcagag atggtcacaa tcccgatgta ttcgcccacg
     8341 aagaacagac cgaacttcat gccggaatat tcaatgtggt aaccatccgc cagttcctgc
     8401 tcggcttccg gctggtcaaa cgggtgacgg tgacacaccg ccacgcccgc gatggcaaag
     8461 gtaataaaac caaagaattg cgggatgacg ttccacacat gcgcctggct gttgacgatg
     8521 tcggtcatgt tgaatgaacc ggcctgcgcc accacgccca tcagggaaag cccgaggaac
     8581 acttcgtagc tcagggtctg cgcagaagca cgcatcgcac ccagcagcga gtatttgttg
     8641 ttacttgacc agcccgcaaa cagcaccgcg taaaccgcca gaccggccat catcaggaag
     8701 aacaaaatcc cgatgttcag gtcggcaacc acccaacccg gactgactgg cacaatcgca
     8761 aaggccagca gcagcgaggt aaaggcaatc atcggtgcca gggtaaagat gacgcgatcc
     8821 gagaatttcg ggatccagtc ttctttaaag aacatcttga tcatgtccgc gaccagctgg
     8881 agcgaaccgc cccagccaac acggttaggt ccgtaacggt tctggaacag acccagcagg
     8941 cgacgttcgc caaagctcat gaatgcccca caggtgacaa ccaccagcag gatcaccacc
     9001 gctttgagga tggtcagcag gatctcaatc agttccggtg atatccaact cattgttgtg
     9061 cctccttgag atcctcaaga tgcgcgccag ccagcaccgg agcaatgccg gacataccca
     9121 tcggcaagcc cacctgccct gccgtcagtc cttcggcgat ttcaaccggc agcgtgaccg
     9181 tgttgccatc gtaactaaag gagacgcgtg tacccgcgtt cacacccaac ttcgcggcat
     9241 ccgctgggtt gagtttgatg tacggctgcg gcatacggct ctggaagacc ggagcacgct
     9301 gtgacaattc atcgctacca aacaggtggt agtacggcgc gatacgccat ttcccgtcct
     9361 gcggctggaa gcgtgccggt acgctggtga agtaatccag accattttcg ctggtttcaa
     9421 acagacgcac gcccggatcg ccaaagcgca gtttgccgcc cacttcgtcc tggaatttgt
     9481 tccacgcctg cggggagttc cagcctggcg cccaggcaaa cggcacttgc gaacggtgcg
     9541 cggtcggctg gttgttacct tccatcgaga aggtgaacat ggtgtcaata tcctgcggct
     9601 gacgcggctc atgaacgctg atattggcgc gcatggcggt acgaccgctg taacggtgcg
     9661 gttcacgggc cagtttctgc ccacgaatac ggaatgtcgc atccggcgca gcatctttga
     9721 tacccgccag ttccgggatt ttcgccacaa cagcgtcaat cacatggtcg agctgcgtcc
     9781 agtccacttc acggctcagc aaggtgctgt gcagcgagtg taaccaacgc cagctttcca
     9841 gcatgacagt tttgctgtcg taataagccg gatcgtaaac ctggaagaaa cgttgggcac
     9901 ggccttcgtt gttaatcacc gtaccgtcgc tttcagcaaa gctggcagca gaaagtacca
     9961 gatgggcgtt ttccataatc gctgtgcgtt gatggtcaac caccatcacc agcggcgctt
    10021 tagccagcgc agcattcacg cgggtagcgg aagcgtgacg atgcagatcg ttttccagca
    10081 ccaccaccgc gtcggcgcgt ccggtttcca gttcggttaa cgcttcttca agcgatccac
    10141 cgcccataat gcccagcccc atgctgttga cggaacgggc aatcatggtg ataccgacgt
    10201 cagcgccgcg acctttcagg gctttcgcca cgttagccgc agcctgaatc acttcagcgc
    10261 taccggcgtt cgtcccggag ataatcaacg gtttcttcgc acctgccagt gcctgcacga
    10321 tgacgtcgat tttgctttgc agctcaggtt caataccgtc aaccgctggc gcggagttat
    10381 ccagcgcatg ggcgatggca aaacctaaac gcgcctgatc ttcaaccggt gcgcggtaag
    10441 tccacgccgc gatatcatcc agacgggtgt catcaacgtt ggtaacaaac agcggatgct
    10501 tcgcacgttg gccgatgttg aggattgccg caatctgcca gtcagccact ttctgtgctg
    10561 ccgccatttc gcgcgcttta cctttcaccg cctgacgaac tgccagcgcg acgcgcgcgc
    10621 cggtctgggt aacgtcttcg cccagcacca gtaccgcatc gtaagattcg atttcgcgca
    10681 gagccggagt ataaatgccg ccttcacgca gcactttcag cgccagttgc agacgttcct
    10741 gctcaccgtg agcgataccg gtgtagaagt tttcttcgcc caccagctca cgcagcgcaa
    10801 agttgctttc cacgctggcg cgcggagaac caatgccgat cactttcttc gactgacgca
    10861 gaatatctgc tgcgccctgc attgcctgtt cggcgttgag ggtaatgaaa tcatcgccac
    10921 gacgctgtac tggctgacgc ggacgatctt tcaggttgac gtagccgtaa ccgaaacgac
    10981 cacggtcgca gaggaagtag tggtttaccg taccgttgta acggttttcg atacgacgca
    11041 gttcgccata acgctcgccg gggctgatgt tacagccgat ggaacattgc tggcagatgc
    11101 tcggcgcaaa ctgcatatcc catttacggt tgtaacgctc ggagtgcgtt ttgtcggtaa
    11161 atacgccggt cgggcaaatt tcgaccaggt taccggaaaa ttcgctttcc agcgtgccgt
    11221 cttccgggcg accgaagtag acgttatcgt gcgcaccata aacgcccaga tctgtaccgt
    11281 cagcgtaatc tttgtagtaa cgcacgcagc ggtagcaggc gatgcagcgg ttcatttcgt
    11341 gagagatgaa tggccccaaa tcctgattac ggtgggtacg tttggtgaaa cggtagcgac
    11401 ggaagctgtg tccggtcatc acggtcatat cctgaagatg acagttaccg ccttcttcac
    11461 ataccggaca gtcgtgcggg tggttggtca ttaaccactc gaccacactt tcacggaact
    11521 gtttcgcttc ttcgtcgtca atggaaataa aggtgccatc ggaagccggt gtcatacagg
    11581 acatcaccag gcgaccacgc gtgtcttccg cgttttggta ttgcttcacc gcacactggc
    11641 ggcaagcacc gacgcttccc agcgccggat gccagcaaaa gtaaggaata tcaaggccca
    11701 gagacagaca agcttccagc aagttgtccg ctccattgac ctcgtattct ttgccgtcta
    11761 catgaattgt agccattagc atgcttccag ttttctcggt cagggaccga gcgttaatcg
    11821 aaattcggtt accagcgctc tttcagcagg ttcggctgaa tcccattaat caaatgggta
    11881 ttgctgaacg gctgtttgat tcccgcctca aattcttcgc ggaaatattt gatggcgctc
    11941 tgtaacggct ccactgcacc aggtgcgtgg gcacagaaag ttttacccgg gcctaagaat
    12001 cgacacagtt gctcaagtgt ttcgatatct cccggctgac cttcgccacg ctccagcgca
    12061 cgcagaattt tcacgctcca cggcagaccg tcgcggcacg gcgtacacca gccgcaagac
    12121 tcacgggcga aaaactcttc caggttacgc accagcgaca ccatgttgat ctcatggtca
    12181 accgccatcg ccagcgccgt acccagacgg cttcccgctt taccgatact ttcgaattcc
    12241 atcggcaggt caaggtgcgc ttcagtcagg aagtcagtac ccgcgccgcc tggctgccag
    12301 gctttgaatt tcagaccgtc acgcatacca ccggcgtaat cttcgaggat ctcgcgtgcg
    12361 gtggtgccga atggcagttc ccacaggccc ggatttttca cccgaccgga gaagcccatc
    12421 agcttggtgc cagcatcttt acttttcgag atgttctgat accactccac gccgttagcg
    12481 aggatcgccg gaacgttaca cagggtttcg acgttgttga cacaggtcgg tttgccccat
    12541 acgccggagg ttgccgggaa ggggggtttc gagcgtgggt tagcacgacg cccttccagg
    12601 gagttgatta acgctgtttc ttccccgcag atgtagcgcc ctgcaccggt atggacgaac
    12661 agttcgaagt cgaaacctgt tcccataatg tttttgccaa gcaagcccgc ttcagtcgct
    12721 tcggcaatgg cgcggcgcag attcactgcc gcttcaatat attcgccacg caggaagatg
    12781 tagccacggt aagctttcag cgcaaacgcg gagataagca taccttccac cagcaggtgc
    12841 ggcagttgct ccatcaacag gcggtcttta taggtgcctg gctccatttc atcggcgtta
    12901 cacagcaggt aacggatgtt catggattcg tctttcggca tcaggctcca tttcaggcca
    12961 gtcgagaagc ccgcgccgcc gcgccctttc agaccagcgt cttttacctg attaacaatt
    13021 tcgtccggag acagcccggt cagcgcctta cgcgcccctt cgtaaccgtt tttgctgcgg
    13081 tattcgtcca gccacactgg ctgtttgtca tcgcgcagac gccaggtcag cggatgcgtt
    13141 tcgggagtac ggataatgtt tttcatttat accgctccag cagttcaggg atcgcttccg
    13201 gggtcagatg cgcgtgagtg tcctcatcga tcatcatgtt tggcccttta tcacagtttc
    13261 ccaggcagca agttggcagc agcgtaaagc ggccatcaaa tgtcgtttgc cctggtttga
    13321 tgttcagctt tttctcgagc gccgcctgaa taccctgata accgttgata tgacagacca
    13381 cgctgtcgca ataacggatc acatggcggc caaccggctg gcggaagatc tggctgtaga
    13441 acgttgccac gccttcgacg tcgctcgccg gaatacccag cacatcggcg atcgcgtgga
    13501 tcgcgccatc cggcacccag ccacgctgct tctgaacgat tttcagcgct tcaatggacg
    13561 ccgcacgcgg gtcttcgtag tggtgcatct cgtgctcaat cgcttcacgc tctgccgcac
    13621 tcagctcaaa agcctcggtt tgtggttgtt gattctcgtg cataattagc ggtccacatc
    13681 tgacataaca aaatcgatac tgcccagata aacaatcagg tcagacacca ggctgccgcg
    13741 gatcgccgcc ggaatttgct gcaaatgcgc ataactcggc gtgcggatac gggtgcgata
    13801 actcattgtg ctgccgtcgc tggtcaggta gtaactgttg atccccttgg tcgcctcaat
    13861 catctggaaa gattcattgg ctggcatcac cggcccccac gacacttgca ggaagtgggt
    13921 gatcagggtt tcgatatgtt gcaacgtgcg ctctttcggc ggcggcgtag tcagcggatg
    13981 atccgctttg aacgggcctt ccggcatgtt gttgaggcac tgctcaagaa tgcgcagact
    14041 ctggcgcagc tcttccactt tcagcattac gcgggtgtag cagtcagaaa caccaccacc
    14101 caccgggatt tcaaagtcga agttttcata gccagaataa ggacgcgcct tacgcacgtc
    14161 gaagtcgatc ccggtggcgc gcaggcccgc gccagtggtg ccccactcca gcgcctcttt
    14221 cgcgccatag gcggcaacgc cctgggaacg acctttcaga atggtgtttt gcagcgccgc
    14281 tttctcgtaa gacgccagac gtttcggcat ccagtcgagg aactcacgca gcaggcgatc
    14341 ccagccgcgc ggcaggtcgt gcgctacgcc gccaatacgg aaccacgccg ggtgcatacg
    14401 aaaaccagtg attgcttcca ccagatcgta aattttctga cgatcggtaa aggcgaagaa
    14461 cactggcgtc atcgcgccga cgtcctgaat aaaggtggag atatacagca gatggctgtt
    14521 gatgcggaac agctcagaaa gcattacgcg aatgacgtta acgcgatccg gcacggtgat
    14581 cccggccagt ttctctacag ccagcacgta aggcatttcg ttaacgcagc cgccgaggta
    14641 ttcgatacgg tcggtatacg gaatgtagct atgccaggac tggcgctcgc ccattttctc
    14701 cgcaccacgg tggtggtagc cgatgtctgg tacgcagtcg acaatctctt cgccatcgag
    14761 ctgcaaaacg atacggaaag ccccgtgcgc cgacgggtgg ttcgggccga ggttgaggaa
    14821 catgaagtcc tcgttttcgg tgccgcgctt catcccccac tcttccggtt tgaaggtcag
    14881 ggcttccatc tccagatcct gtttggcttt ggtcagctca aacggcgaga attcggtagc
    14941 gcgcgccggg taatctttac gcagcgggtg acctttccag gtttgcggca tcatgatgcg
    15001 gcgcaggttc gggtgaccgt cgaaagtaat gccaaacaga tcccaggtct cacgctcata
    15061 ccagttagcg ttcgggaaca gtttggtgaa ggtcggtacg tgcaggtcgt tttctgccag
    15121 cgccaccttc agcatgatgt cgcggttacg atcgatagaa atcagatggt agaaaacgga
    15181 aaaatccgcg gcaggtaatc cttcgcggtg tgtgcgcaga cgttcgtcca tgccgtgtaa
    15241 gtcaaacagc atgacgtaag gtttcggcag tttctttaag aaatcgccaa cttccagtaa
    15301 ttgttcacgc ttgatccaca caacgggaac cccggtgcga gtcgcctgaa cagtaaaggc
    15361 atccggccca aaacggttgc gcagttcgcc aatcaccgga tcatcaagat gatcgcgggt
    15421 ctgccaggcg ggttcttgcg cggttaagtc ggtcatattg ttcaccattg caaatggtcc
    15481 gtggtgactg tcggcgcagt aagcttcgcg aaatagattt aggaataagc gaagaaaaat
    15541 ccctttgccg acaggcgcaa attaaatctc gtcaggtgta cgcaggttgg ttacggcaat
    15601 gcgttcaccg cgcttgcgtt cgcgctctga ttgcatattg gcgcgataaa cgccctgatc
    15661 gccaaccacc caggagagcg gacgacgttc tttaccgata gattcctgca acagcatcag
    15721 tgcctgcatg tacgcttcag gacgcggcgg gcagcccggg atatacacat caaccgggat
    15781 gaatttatcg acgccctgca caacggaata aatatcgtac ataccgccag agttggcaca
    15841 ggcacccatt gagataaccc attttggctc cagcatctgg tcatacagac gctgaataac
    15901 cggtgccatt ttggtaaagc aggttcctgc caccaccatc aggtcagcct gacgcggcga
    15961 agctcgcaat acttctgcgc caaaacgcgc cacgtcatgc accgcggtaa acgaagtcac
    16021 catctcaacg taacagcagg aaagaccgaa gttatacggc caaattgagt ttttacgacc
    16081 ccagttaacc atgtcattga gcttgcccat aaacacgttt ttgttaactt cttgctccag
    16141 agggtcggtt acgatctcct gcttttgcag ggggtaacgg tcgttctcac cgttgggatc
    16201 tatgcgggtg agcgtataat ccatcttaat gcctcgcggt tagcgttgac gattagcgat
    16261 actgttcgtt tccgggttca tacgctcgcg gcgtgaacgc gcgggcgtcc agtccagcgc
    16321 gccaatacgc accagataaa ccagacctgc cagtaacaca aaaataaaaa ttgcagcttc
    16381 cacaaagcct acccagccgc tttcgcggat agaggttgac catgcgaaca gatacagcgc
    16441 ttcaacgtcg aagataacga agaacatggc caccagataa aacttggcgg acaggcgtaa
    16501 gcgggcggag ccgaccgagt cgataccgga ttcaaacggc acgtttttcg acctcgcgcg
    16561 tgcgcgaccg cctaaaaacc aaccgcctac cagcatcagg caacacaggc caatggcaac
    16621 gataagaaag atagcgaatg cccagtgatg agcgatgact tcagtggatg ttgacatact
    16681 cattgcttac tcatcaaaag tagcgccaga ttcactgctc ttcacggcag atggacgcca
    16741 catcgattca tggggaggaa taaaaaaaac cttacaatca ctgtagaaat tcttttatac
    16801 agctaattga tgtggtcttt tactcctttc tataaccttt tgtcaacttt aacaaaagtt
    16861 tcttcacatt agtttacata atatcaacac cattagcatt taatgccctt tcaccccaga
    16921 tccttgacga ctccaggata attagatgtt gttgaatcgt gtccgttgtg aagcaatgga
    16981 aaaaatacgg gtctattttg acaggaattt gtgtcgattc ctccccccaa aagagagtat
    17041 tttcttgatc tgtgacacgc ttttgtcatt ccataacaaa aacgcagcaa caaatttacg
    17101 tattttttaa catcattgta gcaggtgatt tttttcaggc gattatttgt gcgttcggga
    17161 cgtgaatctc tggtgattga aaaatgaaca gttttgtacg ttctgcacga tacgaggaag
    17221 gctttttact aaaaaaaagc cgctggggtt taaaacaccc ccagcggctc gtttttacac
    17281 tattgtctca gaaattatct atggtccgtc gattactcga tatccctttc aatcaacaat
    17341 gagtcatcgc cttccggagc agacattggg ctgtattgcc acggattatg gtagctctcc
    17401 atcgcctgat agatcacctg ggccagctca ttattactgg acgggtcata gcacagcaaa
    17461 tattcggtat cgggtagcgg cggtaagcca tctactccac tcagcacacg caggtctgga
    17521 ctcatcatct cgaccggcct tgccgtcacg ccaagaccgg cttttactgc cgcacgaact
    17581 gccggaagcg tcgaggcgac ataagccagt cgccatggaa tatctgcttt attaagcgtc
    17641 gccagcacca tatcgcgaaa cgggctagga tcatccagca atacaagagg gatcggctcg
    17701 cctttttgca atacgtattc cgctgcgcag taccagtgtg ttggcgaggt acgcaggttc
    17761 aatgccttaa acgttgaagg acgatgggtg gttaccatca aatccacttc ctgggattcc
    17821 agcatttctg ccatataggc attacgttta acgcggacat ccagcgctag tttcggataa
    17881 accgaactca cgcgatttaa caggaaaggt aagatcgtat cggcagattc atctgaagca
    17941 ccgatagtta acacgccctg aagattactg aacattaatg atgagcaggc ctcatcatta
    18001 aaacgcagaa tttttctggc gtaaccaaga agttgaatgc catgttcagt taacagtttg
    18061 ttgcgaccgt gacgagcgaa cagttctttc ccaacgagtt gttccagacg ctgcatttgc
    18121 tgacttacgg cggactgagt acgacacaca gcggcagctg cggcagcaaa agtgttcaga
    18181 tcggcaacag caacaaatgt tctcagcaga tcgaggtcga ggttaattat cggacgattt
    18241 gcacttatca tatattatca cttactggcg gctcatactg agctggttaa tgctgtgcac
    18301 acacaaacaa gcaattccat ttgtaatgtg cctccctggc agtttcatcc ggaacccgga
    18361 cgaaagtaaa aatgcatatg agttgcacta aaaaagcgac tcacattgtt ccgttataat
    18421 gcctgaagta gatcacagaa tatatcttca ggaatcacat atctattaag ttactcactc
    18481 ttttgtattt atgacatgcg cgtgtttgta taaatgtaaa tgtgagtcct tgttccactc
    18541 tcgtgcagca tcgctggtca tacgcgaaca cgtacaaaca gcaatggtga ggcggcatca
    18601 agagcaggga tccgtcattt atgcgagcat tttaccccaa aaacctttta tttataaggg
    18661 tcattgcgaa ttatctgatg caaagtt
//
LOCUS       DAPHCV010000097        18403 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.097, whole genome shotgun sequence.
ACCESSION   DAPHCV010000097 DAPHCV010000000
VERSION     DAPHCV010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.097"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..903)
                     /locus_tag="RH033_003484"
     CDS             complement(<1..903)
                     /locus_tag="RH033_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992616.1"
                     /translation="MSIYFTPCMINTYHGDIYNRTTDTDNVKTPDTPMWPCDNQEEQQ
                     PINFSLSEEGYNAEQFDLAQQQLQQINACHTNTTYTNADYSKVVAQLVKLINNIETIS
                     STPLTQQTQSILNQINNIRYEKNKNSECRIIVVANPKPDKAIITKISVEEGMPVRFSV
                     QTVSSENKNFSGQRTDLPTDIKDIQSLYQKMTRLYIEHSENENRMKVFAGTNFIDFNV
                     TGQDLSGFVLTLSRFYFEDLLNINFTAANLGDTIFSHKEHPTPKLYKDEQYLDKQIEG
                     LFSTLLTINDNFLRAKAEIASTIIK"
     gene            complement(1484..2485)
                     /locus_tag="RH033_003485"
     CDS             complement(1484..2485)
                     /locus_tag="RH033_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDV2992617.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGKGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWVTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIVSKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIEKPVVNCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSEWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRIPMIVAFLTVILGMAILMGYLTQMLF"
     gene            2593..2889
                     /locus_tag="RH033_003486"
     CDS             2593..2889
                     /locus_tag="RH033_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV2992618.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(3023..3448)
                     /gene="arsC"
                     /locus_tag="RH033_003487"
     CDS             complement(3023..3448)
                     /gene="arsC"
                     /locus_tag="RH033_003487"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HDV2992619.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLADDKFTDDRLIDFMLQHPILINRPIVVTTLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(3461..4750)
                     /gene="arsB"
                     /locus_tag="RH033_003488"
     CDS             complement(3461..4750)
                     /gene="arsB"
                     /locus_tag="RH033_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HDV2992620.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGGTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLTALALRLSFTL"
     gene            complement(4804..5157)
                     /gene="arsR"
                     /locus_tag="RH033_003489"
     CDS             complement(4804..5157)
                     /gene="arsR"
                     /locus_tag="RH033_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HDV2992621.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPSWAAKIIEQAWRCEQEKVQVIVRN
                     LARQNCSGDSKNICS"
     gene            complement(5405..5620)
                     /locus_tag="RH033_003490"
     CDS             complement(5405..5620)
                     /locus_tag="RH033_003490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992622.1"
                     /translation="MMAISLQSEPPTQGWKVKNRRKVGFLHPDSDKAWYGSDPMKVYK
                     SSFTNMYSKLSLRQLKKSLTLAMLATG"
     gene            5899..5982
                     /locus_tag="RH033_003491"
     CDS             5899..5982
                     /locus_tag="RH033_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HDV2992623.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(6036..7388)
                     /gene="gorA"
                     /locus_tag="RH033_003492"
     CDS             complement(6036..7388)
                     /gene="gorA"
                     /locus_tag="RH033_003492"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDV2992624.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(7460..8302)
                     /gene="rlmJ"
                     /locus_tag="RH033_003493"
     CDS             complement(7460..8302)
                     /gene="rlmJ"
                     /locus_tag="RH033_003493"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HDV2992625.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLSSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            8505..10547
                     /gene="prlC"
                     /locus_tag="RH033_003494"
     CDS             8505..10547
                     /gene="prlC"
                     /locus_tag="RH033_003494"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HDV2992626.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMELFKRFRGREPQLDAMLEHYGIKG"
     gene            10555..11307
                     /gene="rsmJ"
                     /locus_tag="RH033_003495"
     CDS             10555..11307
                     /gene="rsmJ"
                     /locus_tag="RH033_003495"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HDV2992627.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(11356..12825)
                     /gene="dtpB"
                     /locus_tag="RH033_003496"
     CDS             complement(11356..12825)
                     /gene="dtpB"
                     /locus_tag="RH033_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HDV2992628.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGLSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(13140..13574)
                     /gene="uspA"
                     /locus_tag="RH033_003497"
     CDS             complement(13140..13574)
                     /gene="uspA"
                     /locus_tag="RH033_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HDV2992629.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            13965..14300
                     /gene="uspB"
                     /locus_tag="RH033_003498"
     CDS             13965..14300
                     /gene="uspB"
                     /locus_tag="RH033_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HDV2992630.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(14371..15870)
                     /gene="pitA"
                     /locus_tag="RH033_003499"
     CDS             complement(14371..15870)
                     /gene="pitA"
                     /locus_tag="RH033_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HDV2992631.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            16102..17295
                     /locus_tag="RH033_003500"
     CDS             16102..17295
                     /locus_tag="RH033_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDV2992632.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGITWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLVAE"
     gene            complement(17382..>18403)
                     /locus_tag="RH033_003501"
     CDS             complement(17382..>18403)
                     /locus_tag="RH033_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2776 family protein"
                     /protein_id="HDV2992633.1"
                     /translation="IPLLMGIICIALGEFVLTGSGQSEYFVAGHVLISLSAICLALFT
                     TAFIIISQLTHGMNKFYNRLFPVIGYAGSATTMIWGWSLLASNNVMADEFVAGHVIFG
                     VGMIAACVSTVAASSGHFLLIPKNASGSKSDGTPLQAYSSTIGNCLIAVPVLLTLFGF
                     IWSVILLRSADITPHYVAGHVLMGLTAICACLIGLVATIVHQTRNTFSVKEHWLWCYW
                     VILLGSLTIIFGIYVLISSDASARLAPGIILICLGMICYSIFSKVWLLALVWRRTCSL
                     ANRIPMIPVFTCLFCLFLAAFLAEIAQTDMAYFIPSRVLVGLGAVCFTLFSIVSILEA
                     GSAKK"
BASE COUNT         4818 a         4527 c         4662 g         4396 t
ORIGIN      
        1 tttaattatt gtcgaagcaa tttccgcttt tgctcgtaaa aagttgtcat taatggttaa
       61 taatgttgaa aataaacctt cgatttgttt gtcaagatat tgttcgtctt tatataattt
      121 tggggtaggg tgttctttat gtgaaaaaat agtgtcacct aaatttgcgg cagtgaaatt
      181 tatattaagt agatcttcga aataaaacct tgacaaggtt agaacgaacc cggaaaggtc
      241 ctgtcctgtc acattgaaat caataaagtt ggttccagca aagactttca tcctgttttc
      301 attttcactg tgctcaatat acaaccttgt cattttttgg tacaagcttt gaatatcttt
      361 tatatcagtt ggtaaatcag ttcgttgccc agaaaaattt ttattttcgg aagaaacggt
      421 ttggacactg aatcttactg gcattccctc ttctacgcta attttagtta tgattgcctt
      481 gtcgggttta ggattagcga caacaatgat tcgacattca gagtttttat tcttttcgta
      541 tctaatgtta ttaatctgat taagtattga ttgtgtctgt tgcgtaagtg gtgtcgaact
      601 tatcgtttca atgttattaa taagttttac aagttgagcc acaacttttg agtagtctgc
      661 atttgtatat gtggtgttcg tgtggcacgc attaatttgt tggagttgtt gttgcgctaa
      721 atcaaattgt tcagcgttat atccttcttc agacaatgaa aaattaatgg gttgctgttc
      781 ttcctgatta tcacaaggcc acatcggtgt gtctggtgtt ttaacattgt cggtatctgt
      841 tgtgcggtta taaatatcgc cgtgatatgt atttatcata catggggtaa agtaaattga
      901 cataacattc cttattgttc atctatttcg ctaaattttt gtaaatcgga gaattagagc
      961 aggaatggat tttcttctac gcggaaataa acacaatgcg tcatatcttc ctgatataat
     1021 tacctttaac ttcactttca tttccacccg gtaatatttg tgtataaaaa aagggcagtg
     1081 atagtatcgt aaaggaaaac gttaacaact atcaaaaagc gctcatttta aaaagttatg
     1141 atgttcaccg atagtgtgtg aagtgatatt tttgtgatgt tttagatgtg taataatgaa
     1201 ccaggttaaa gtgtatttaa aaatgtgttg tattggcgac tgaaatctgc cctttaaaag
     1261 gggggatagc actgaaacaa agagacattg cttctccgat aaaatgtcag agaagcatga
     1321 ttgcgtggtg attgtcgatt gatgacttac tgacggatcc gtcatcattc tcaaagcatt
     1381 agttaagtta tttgtgattt ttaaggatta tcctatctat gattttctac tcagaaggat
     1441 aaggcactca caatgctcct tatagtttac tgctggcatc tatttaaaat agcatttgag
     1501 tcaaatagcc catcaagata gccataccta atataaccgt caggaatgca actatcatcg
     1561 gtattctgaa cattgattta agtaatatca cttccgtcag gcttgcgccg gcactgccga
     1621 tgattaatgc cattaatgct cccagaccca ttccttttgt catcagaaca gatgccagag
     1681 ggataaccgc ctctgcccgg atatatagcg gaataccgac aacggcgctc aatgggatgg
     1741 cgaagggatt atctgcccct gcatgagcgg caatccactc tgagggaata aagccataaa
     1801 taaaagagcc tattaatacc cctaacaaaa gatatggcag tacatcttta aactgttgca
     1861 acgcatcttt ggtggctagt tttatgggat taatacttac cacagctttg gtactgcaac
     1921 agtttactac aggtttctca atggctttag ccgctggact acagcaactc acttttattg
     1981 gcgtatatgt cgtccccggc gaagttttgg ctggagcaca acaatttgct gatgagcttt
     2041 tactgacaat gatatgacgt tcaaatccca gagaatccag gataatactg gcaagtacgg
     2101 agacgccggc ggcgataatc gcgtacaaca aggtaacttt ccagccaaag gtcacccaca
     2161 ttagtccgac gataatggga ttaagtaatg gtgaaacaaa taaaaaagtg agagtcggac
     2221 cgaacccggc tttcgctgac aacaatccac gtaacattgg gattgtcgag caactacaga
     2281 acggggtgac ggctcccaac agagcagcca ggagatagcc tttccctttt cttgccccca
     2341 tcatctgctg gattttatgg tctggcacct tttgtcttat caggctgaca ccggcactaa
     2401 tcactataaa cagcaaagaa agctcgacgg cgagaaacac aaacatctct gcggcatctt
     2461 gcagcatcgc aagccaacta ctcatacata acctcattga ataaatctat atttccagaa
     2521 taatcgaaat gaaattcaga tcaagatatt tctggtatta tcgaaatatc aaccagacaa
     2581 agggggtgtc atatgcagtt ggaagaagtt gctaaagcgc tgaaagagct aggtcacccg
     2641 acccgtttgt tcatattcaa acacctggtc aaagcggggg aacaaggttt gccggtcggt
     2701 gagttgcaaa agcagcttgg gatacctggc tccacactca gtcaccatat atcagcactg
     2761 gtatcggtag ggctggtcaa acaaaaccgt gagagtcgga cgctgatgtg tgtttcgcaa
     2821 tacgcaatgc tggaagcgat aattgaattc ttacgcgagg agtgctgcgt aaatagcaaa
     2881 attgcctgat gcgttacgct ctacatattt catgccatat attgaattag cacggttttg
     2941 taggtcggat aagacgcgtt agcgtcgcat ccgacataaa caaagcgcac tttaccaact
     3001 atcggcagtg gatatcgccg ctttatttca ggcgcttacc cgcttcatca accactttct
     3061 cgccatcttc cttggagaat gcgccttttt gcgcatctgg cagaatttcc agcaccactt
     3121 ctgaagggcg gcacagacga gttcccagcg tcgtcaccac aatcggacgg ttaatcagga
     3181 taggatgttg cagcataaaa tcaattaacc gatcgtcggt aaatttatcg tccgcaaggc
     3241 ccagttcctc atacggttcg acgtttttac gcagcagcgc gcgtacggaa atccccatat
     3301 cggcaatgag tttgaccagt tcatcgcgcg ttggcggagt ttccagataa tggataatgg
     3361 tcggttctgt gccgctgttg cggatcatct ccagcgtatt acgcgacgtg ccgcaggccg
     3421 ggttgtgata aatggtaatg ttgctcatat caatatctca ttacaaagtg aaagagagac
     3481 gtagcgccag cgcagtcagc gtcacaaaca gcacaggcag ggtcatgata atccctgtac
     3541 ggaagtaata tccccagctg atagtcatat tcttctgcga aagtacgtgc agccagagta
     3601 gcgtagccag gctaccaatt ggggtaattt tcggtcccaa atcgcagcca atcacgttgg
     3661 cataaaccat cgcttctttg ataacgccag atgccgtgcc gccatcaatg gacaacgcgc
     3721 caaccagtac cgtcggcata ttgttcataa tagaagagag gaaggcggtg aggaatccgg
     3781 tgccgagcgt cgcggcccac aggccgttat ccgccagcac gttgagtacg ccagaaagat
     3841 attccgttaa tccggcattg cgcaggccat aaaccaccag atacatgccg agcgagaaga
     3901 tgacaatctg ccagggtgca ccgcgcagga ctttacccgt attaatcgca tgaccgcgtt
     3961 tagcgacgac aaataatatc agtgcgccca ctgccgcaat ggcgcttacc ggaatgccga
     4021 gtggttccag gacgaaaaat cccaccaaca gaagcagtaa aacaacccag ccagttttga
     4081 acgtagcagg atctttgatc gcttctgcgg gagatttcag cagcgccata tcgtagttct
     4141 gcggaatatc tttgcgaaaa tagagatgta gcatcaccag cgtggcaaca atcgcggcga
     4201 tatccaccgg caccatcacc gaggcgtatt cgcgaaagcc gaggccaaag aaatcagcgg
     4261 aaacgatatt caccaggttg gagacaataa gcggcaggct ggcggtatcg gcaatgaatc
     4321 cggccgccat cacgaacgca agcgtcgtgc ctttactgaa tcctaaagcc agcagcatgg
     4381 cgatgacaat tggcgttaaa atcagcgccg cgccatcatt ggcaaacaga gctgcaacgg
     4441 cagcaccgag caggacaatc caggtaaaca gcaagcgacc gcgtccattg ccccagcgtg
     4501 agacgtgcag cgccgcccat tcaaaaaagc cggactcatc cagcagcagg ctgatgataa
     4561 tgacggcaat aaacgcagcc gtcgcgttcc agacgatatt ccacaccacc ggaatatcac
     4621 ccggatgtac cacgcccgta actaacgcca gtaccgcgcc gagcgttgca ctccagccga
     4681 tgcctaaacc tttcggctgc cagataacca atacaatggt caggacaaag atagcgcctg
     4741 ccagtaacat aatgcctcct ggataaaaca catgcgaaaa tacatatgtg tttagctaaa
     4801 tttttaactg caaatgttct tactgtctcc ggaacagttt tgtcgagcca ggttgcggac
     4861 aatcacctga accttttcct gttcgcatcg ccaggcctgc tcaataattt tcgcggccca
     4921 tgatggaata tgcggtgata agcggtaatg aacccactta ccctgcttgc gatccagcaa
     4981 tagcccactt tcacgcagca atgccaggtg gcgggatatc ttgggctgcg actgatcgag
     5041 ggcagtacag agatcgcaga cgcataattc tcccagctcg ctgagcagta aaacgatgcc
     5101 cagacgggtt tcatcagcaa gaattttgaa cagttggatg ggtaacagaa atgacatatt
     5161 gcgctcctga ttgttgcagg tagtgtctct cttcgaagcg gataagtcaa aaacatatat
     5221 ggtttaacga atgtgtatgt gcagaggaag gtaataggtg tgaattttga gttggctatt
     5281 catttgaaag gaggtctgaa tcaggacgta aaaaaaccga ctttgcgtcg gttttttact
     5341 ttccagccct gagttggtgg ctctggctgg agtgagaatg cccacatcta acggttcaat
     5401 actattagcc agtggctaac attgcaagcg ttagcgattt cttcagctgg cggagggaca
     5461 gtttgctata catgttggtg aaagaggatt tatatacttt catcgggtcg ctgccgtacc
     5521 aggccttgtc cgaatccgga tgtaaaaaac cgactttgcg tcggtttttt actttccagc
     5581 cctgagttgg tggctctgac tggagtgaga tagccatcat ctaacgcatc aatgctaata
     5641 ccatactgaa actattgcaa ggacatgctg gttttataac ccgcaagtac tgtatgatta
     5701 tccagttagc tctgagacat tttcactctg gcaatgcgca taaacgcttt caaagtcctg
     5761 gtcagaagta cgggtggtgc cgttaactga tgctctggcc ggagtgagaa tgcccacatc
     5821 taacaatggg gcatgcgccg tgacaggcag tggatgagta agcggatgca ttctcactcc
     5881 atcgcatgga gaaaacgggt gattgataaa gcaatcatcg ttcttggggc gttaattgcg
     5941 ctgctggaac tgatccgctt tctgcttcag cttctgaact gatagcggaa acgtagctaa
     6001 gggctaagag cacactactc ttagcccttt aacatttaac gcattgtcac gaactcttct
     6061 gccgctgtcg ggtgaatggc aaccgtgttg tcgaagtctt ttttggttgc gcccatcttc
     6121 agcgccaccg cgaagccctg caacatttca tccataccaa aaccaatgcc gtgaattccg
     6181 acaatcttct cttccggtcc aacgcatacc agtttcatgc ggcacggctg gcggtgagtg
     6241 gtgacggcgg tatacatcgc ggtgaaagag gatttataca ctttcacctg atcgtcgccg
     6301 tactgctcgc gcgcctgcgg ttccgttaaa ccaacagtcc caatcggcgg atggctgaag
     6361 accacggtcg gaatgttgct gtaatccaga tgctcatccg gcttgttatt aaacaggcgt
     6421 tcagagagac ggcgacccgc tgcaactgcc accggtgtca gttccactgc ccccgtgtta
     6481 tcgcccaccg cgtaaatacc ttcaacgttg gtattttgat atttatcgac gacgatatag
     6541 cctttttcgt tagttttaac gccagcggct tccaggttga tgttgtcatt ggcaggctcg
     6601 cgaccaatcg cccaaatcag gcaatccacc gtttcactgc gcccatcttc cagctccagc
     6661 gtcaggctac catcagcatt tttcactact gctttcggga tggcgttggt atgcaactgc
     6721 gggccttcgg cgttcatcac ttcgaccagc gtttcagaga tcatcgggtc gaagctgcgc
     6781 agcggcgcat gtttacgcac aaacagatgc gttttcgcgc cgaggccgtt aatcacgccc
     6841 gccagttcca cggcgatgta acctgcgcca acaaccgcca cgcgctctgg caaagcagga
     6901 agggcgaaga agccatcaga atcaataccg tattccacgc ctggaatatc cgggtggctc
     6961 ggacgaccgc cggtggcgat cagaatatga tcggccgtga tggtttcgcc gtttacctcc
     7021 agcgttttgg catcaacgaa gcgggcaaag cctttgatta catcaacgtt atttttaccg
     7081 agcacgtttt cataggaagt atgaatacgg tcgatatagg cggtacggct ggcgatcaac
     7141 gtttcccagt tgaatttatt gatagtggta tcgaaaccat aatccggacc gtacatgtgg
     7201 atcgcttcac ggatttgcgc cgcgtgccac atcacttttt tcggcacgca gccgacgttc
     7261 acgcaggtgc cgcccagttc tttggcttca atcagcgcgc atttctgacc gtacatagcc
     7321 gcgcggttga tggaggcgat accgccgctg ccgccgccga tggcgatgta atcatagtgt
     7381 ttagtcatga catattgtcc ttatcgttga ttaccgcgat tgtagcgcga gacgtaatag
     7441 gtgccagcga tggctgcaat tactccggca cgatccagct tacggtggcg tgcccagtgc
     7501 cagctggaac cagtttgctg tgcagccacg gcagcacgtt attcatctgc tgttccagct
     7561 tccacggcgg attaatcaca atcatgccgg aagcggtcat gccacggcga tcgctgtctg
     7621 gcagcaccgc cagttcaatt tgcagaattt tgcgaatgcc ggtcgcttcc aggtcgtgga
     7681 tcatgcgctt aatttgctgg cgcagcacca ctggatacca caatgcgtaa gtgccagtgg
     7741 cgaaacgttt gtaaccttct gctatcccgc tgaccaccgc ctgatagtcg gttttcattt
     7801 catacggcgg gtcgataagg attaaaccac ggcgggaaac cggcggcagc ttggccttaa
     7861 gctgctggaa accgtcggct ttttcgacgc gcgcacggct gtctttctga aattcagaac
     7921 gcagcaacgg gtaatcgctc gggtgcagct cggtcagttg caggctgtcc tgttcacgca
     7981 gtagctggcg agcaatcaac ggcgaaccgg ggtaataacg caactgcccg ctacggttga
     8041 agtgttttac cacattgatg tacgcctcca gctccgcagg caaatcgtcc tgctgccaga
     8101 tacgggcgat accttcgaga tattcgccgg tacgctcggc atgttcgctg cttaactgat
     8161 aacgcccggc ccctgcgtgg gtgtcgagat agagaaacgg tttatctttc tctttcagcg
     8221 actcgatgat caggctctga acggtatgtt taaggacgtc ggcgtggttg ccagcgtgaa
     8281 agctgtggcg ataactgagc atgggtaaag gtgttccggt aagtaaaaat cggcccgtat
     8341 tcggggcgca caaaagccta tcaggacagt ataccgaaaa gaggccgccg ccgcgaaagc
     8401 gtaacgtttc tcattgaaat tcactacact taaccccatg ctacacacat tatgtaaagc
     8461 gcctgttgag cgcttcctta acctctttaa ccaggactgc gctaatgacg aatccgttac
     8521 tgactccctt tgaattgcct ccgttttcta aaattctccc ggaacatgtc gttccagccg
     8581 tgactaaggc gctgaatgac tgccgcgaaa acgtggagcg cgtagtagcg caaggggcac
     8641 cgtacacctg ggaaaatctc tgccagccgt tggcagaagt agacgatgtg ctggggcgta
     8701 tcttctcccc ggtcagccac ctgaactcgg tgaaaaatag cccggaactg cgtgaagcgt
     8761 acgaacaaac cctgccgctg ctgtcagaat acagcacctg ggtagggcaa catgaagggc
     8821 tgtataaagc gtatcgcgac ctgcgcgatg gcgatcatta cgccacgctg aacacggcgc
     8881 agaaaaaagc ggttgataac gcactgcgcg acttcgaact ctctggcatc ggtctgccaa
     8941 aagagaaaca acaacgctac ggtgagattg ccacccgtct ttccgagctg ggcaaccagt
     9001 acagcaacaa cgtcctcgat gcgacaatgg gctggaccaa actcgttacc gacgaagcgg
     9061 aactggcagg aatgccagaa agcgcactgg ctgcggcaaa agcccaggct gaagcgaaag
     9121 agctggaagg ttatttgctg acgctggata tcccaagcta cttgccggta atgacttact
     9181 gcgacaacca ggccttgcgt gaagagatgt atcgtgctta cagcacccgc gcttccgatc
     9241 aaggcccgaa cgccggtaag tgggacaaca gcaaggtgat ggaagagatc ctcgcgctgc
     9301 gtcacgaact ggcgcaactg ctgggttttg aaaactacgc ctttaaatcc cttgccacta
     9361 aaatggcaga aaatccgcag caggtgcttg atttcttaac cgatctggca aaacgcgcgc
     9421 gtccgcaagg tgaaaaagag ctggcgcaac tgcgtgcctt cgctaaagcc gaatttggcg
     9481 tcgatgagtt gcagccgtgg gatatcgctt actacagcga aaaacaaaaa cagcatctct
     9541 acagcatcag tgacgaacag ctgcgtccgt acttcccgga aaacaaagcg gttaacggct
     9601 tgtttgaagt ggtgaaacgt atttacggca tcaccgctaa agagcgtaaa gatgttgatg
     9661 tctggcatcc ggatgtacgt ttcttcgaac tgtatgacga gaacaacgaa ctgcgcggca
     9721 gcttctacct cgacctgtat gcccgtgaaa acaagcgcgg cggggcgtgg atggatgact
     9781 gcgtaggcca gatgcgtaaa gccgacggtt cgctgcaaaa accggtcgcg tatctgacct
     9841 gtaacttcaa ccgcccggta aatggtaaac cggcactgtt tacccatgac gaagtgatca
     9901 ccctgttcca tgaattcggt cacggcctgc accatatgct gacccgcatt gaaactgctg
     9961 gtgtatctgg tatcagcggg gtgccgtggg atgcggtcga actgccgagt cagtttatgg
    10021 aaaactggtg ctgggagcct gaggcgctgg cgtttatctc cggtcactat gaaaccggcg
    10081 aaccgctgcc gaaggagctg ctggataaaa tgctggcggc taagaactac caggcggcgc
    10141 tgtttattct gcgccaactg gagttcggcc tgtttgattt ccgcctccat gccgagttcc
    10201 gcccggatca gggagcgaaa atcctcgaaa ctctggcaga aatcaagaaa ctggttgccg
    10261 tagtaccgtc tccatcctgg ggccgtttcc cgcacgcttt cagccatatt ttcgccggtg
    10321 gttatgccgc aggttactac agctacctgt gggccgacgt gctggcggca gatgccttct
    10381 cgcgttttga ggaagagggc attttcaacc gtgaaactgg gcagtcgttc ctcgacaaca
    10441 ttctgagccg tggcggttca gaagagccga tggagctgtt caaacgcttc cgtggtcgtg
    10501 aaccacagct ggatgcgatg ctggagcatt acggcattaa gggctgaaca ttcagtgaaa
    10561 atctgcttaa ttgatgaaac aggcgccgga gacggcgcct tatctgttct ggcggcccgc
    10621 tgggggctgg agcacgatga agacaacctg atggcgctgg tgttaacacc ggagcacttg
    10681 gaactgcgca agcgtgatga gccgaagctg ggcggcatct ttgttgattt tgttggcggg
    10741 gcgatggcgc accgacgcaa attcggcggt ggtcgcggag aggcggtggc gaaagcggtg
    10801 ggcattaaag gcgattattt gccggatgtg gtggacgcca ccgcagggct ggggcgcgat
    10861 gcctttgtgc tggcttcggt cggctgccgc gtgcggatgc tggagcgtaa tccggtggtt
    10921 gctgcattgc tcgacgacgg cctggcgcgt ggttatgcgg atgcggaaat cggcggctgg
    10981 ttgcaggagc ggttgcagtt aattcacgct tccagcctga cggcgctgac cgatattact
    11041 ccgcgcccgc aggtggtcta tctcgatccg atgttcccgc ataagcagaa aagcgcgttg
    11101 gtgaaaaaag agatgcgtgt ctttcagtcg ctggtggggc cggatcttga tgccgatgga
    11161 ttgctggagc ctgcacgctt gctggcgacc aaacgcgtgg tggtaaagcg cccggactac
    11221 gcgccgccgc tggcgaatgt cgccacgcca aacgcggtag tgaccaaagg gcatcgcttt
    11281 gatatttatg ccggaacgcc ggtgtaaaag taaagcaggg tagcgttatc gctaccctgc
    11341 tttctgcaag aataattaat ggctttcggg cgtcgcaatc atgcgtttca gccacggcac
    11401 catcagcagc atcaccaccg caacgcccag cgtaaccagg ccaatcttac caaacacgtt
    11461 ggtatagacg ggcaacgtct caagcggatc ggtaatgttg tctggtactg cggtaaatgt
    11521 tgccacatag ccgcccagca agaacgcggc agcctgcgtc aggaaccaca tcccgagaat
    11581 aaagcccatc aaatgctgcg gcaccagggc agcaatcatc gccagaccca gggcgctaat
    11641 aaacaattca cccaggctct ggaacaagta aaccagcacg ataaaccacg gcgatgtcag
    11701 tccttgtgca tccgcaaacc acattcccgc agcggcggct gtaagaaagc ccagcgagca
    11761 cataaacatg ccgagagtaa atttcatcgg catcgagagg tctttgcctt tgttacccag
    11821 atgcgtgtaa atgcctgcca gtattgggct ggcgagtacc acccagaacg ggttaagcgc
    11881 ctggaagctg accgggttga tggaaaagcc gagaatttca tgatgcacgt tgttgatggc
    11941 aaagaagttc agcgacgttg gcatctgggc gtagagaatg taaaacacca ccgcttcgag
    12001 catcaggaca aaggcgacaa acattttatt gcgcccggtt ttatccagct tgaatgcctg
    12061 acgaaagaaa atgatggtga cgacgatgga gagaacaatc agcaccagat tggcgacttc
    12121 tacgttgtgc atcagccatg cacagacgaa gatcatcacc acgctgccaa gtaacacgta
    12181 caacagtttg ctgaagctca tcggccggaa gtcgggttca gaaccaatgt ctttcaccat
    12241 tccgcgacag gcgatgtaaa ccaatagcgc gataattaat cccgcaccgc acaggttgta
    12301 ggtgactgaa tagccgaatc tatcagcgat cacaggggcc agcgataacg ctatcaacga
    12361 gccgatgttg atcgacatat agaacagggt aaatgcgcca tcaagccgcg gatctttcgg
    12421 cggatagcac ttcgaaagca agctggctgg gttagcttta aacaggccgt taccgacagc
    12481 gatagtcccc agggcgatga aaatcaggtc aggcttaagt agcgacaggc cggtcatgaa
    12541 gtagccaatc gccagcacaa gtgctccgag gacaatggtg cgtttggtcc ccagcaggtg
    12601 gtcgccgaca tagccgccaa tggaaatgag gccatagacc agcgcagcaa aagcaccaaa
    12661 agtgacaaaa gcctgttctt gcgagaatcc aagctgctta acgaagaaaa ccgccagtac
    12721 gccctgcacg ccgtagtagc cgaatcgctc ccataactcg acaaaaaaga tcatgaaaaa
    12781 tgggcgaggt tgctgcagca tccccatggg tgttgttgta ttcatattta ttaaccttcc
    12841 aataccatcc tgaaaagtat gaacgcgtaa gggataacgc gcgaaaaagt ttacatctgt
    12901 cagagattct taatattgat gcggtgaata gattaataca ctattacaac agaaaataac
    12961 cagctgatca cactaatgat tgaaaatatg atggtgttaa atgttgtatt ggcaggggat
    13021 tttgattgcg tacgcagacc gtggccagat aaggtgttta cgctgcaagg taacaaggcg
    13081 cgccaaaaag caaaacgccc gcatatgcgg gcgttcagga acacgtcgta gagggaagat
    13141 tattcttctt cgtcgcgcag cggaacaatc agcatatcaa cgtgaacggt gttgatcagc
    13201 tgacgtgcgg aagacatcag tttgctccag aagtcctggt ggtgaccaca aaccaccaaa
    13261 tccatatcgt atttcttgat tgcatcgacc agaacctggc ccaggtcgcc gctgccgctc
    13321 agggtttcag tgattgggta gcctgcatta gtggaaagct cggtcagcgc atgatgtgtc
    13381 tcttcagaga tgcgtttctg catatcaccc agattcacat caataagccc ggtgtatagg
    13441 tcagagtagt ttacatctac gtggatcaga gaaactttcg cattgtaggg gcgagccata
    13501 gagactgctt tctctaccag aactttgctt tccggggaga gatcgaccgc gatgagaatg
    13561 tgtttataag ccatagtgtt actccttcca taaagttgtc gatgactggc cagctagcgt
    13621 ttcttgtgcc gttacgtcac ccgcgtcctg cgaactacgt gcgcggggct aacccagccg
    13681 taaagattca gtgttaagac catccttacc ttatagcgac ccggatgatc cgtcaatccg
    13741 ccttgcttac ccgttagata aacaaatttt cgacatgaat atattgatag tggttaacct
    13801 tctggaaaaa aaacaacctg atctcctaca ctatctatag agccgctcgg atgttggtca
    13861 ccacaaaagc ggtttctcca ggacctggtg ttaactgatt ggcagcgtat cgggagcggt
    13921 agccccgggg tggaactccg cgggcaggtc gccggggagg aggtatgata agcaccgtcg
    13981 cattattttg ggctttatgt gtcgtttgca ttgttaacat ggcgcgctat ttctcatcac
    14041 tacgcgcgtt gttagtggta ctgcgtaact gcgatccatt gctctatcaa tatgttgatg
    14101 gagggggctt ttttacctca catggccaac ccaacaaaca ggtgcgtctc gtttggtata
    14161 tctatgccca acgttatcgc gatcatcacg atgatgagtt tattcgccgc tgtgagcggg
    14221 tgcgtcggca gtttattctg accagcgcat tgtgtggtct ggtggtggtc agtctgattg
    14281 cattgatgat ttggcattaa gcacttcagc aaaaataacg cataaaaaaa gcgggccagt
    14341 aaactgaccc gctcgttttg gtgcgtgcga ttacaggaac tgcaaggaga gccagtacag
    14401 cccgccggaa agcagtaccg cagccggaag ggtaaacacc caggccatca gaatactggt
    14461 cacggtttta cgctgtaagc cgccgccatc taccaccatc gtccccgcga cagaagagga
    14521 gagtacgtgg gtagtggaaa ccggcatccc ggtataactc gccaggccga tagacactgc
    14581 cgcagtcatc tgggcagaca ttccctgagc gtaggtcatg cctttcttac cgattttctc
    14641 accgatagtc gttgccacac gacgccagcc aatcatcgta ccgataccta acgccagcgc
    14701 gaccgccatg atgatccaca ccggtgcata ctcgatggtg ctaagcatgt cagacttcag
    14761 tttcttcaac aggcgctgat cgtcagcact cacgccaggc atcttcacca ctttgtcgat
    14821 agtgtcagaa acgcacagca taatgcggcg catctggcta cgttgatcaa gcgacagctt
    14881 atcgtagctt tccacatcgg tagtcaacat gcctttcagg cggttgagcg cgttaatggt
    14941 attcgacgga tggcaatgga actccgcagg ttgcgttgcg ccagcttccg gagccggtac
    15001 taactgatca gcaccggtcg cttgtttcag cagcgcagga tgctgctcaa agtaagcttc
    15061 gacgttgttg atggcatcac gggtacgggt gatttcgtag ccagtggcat tcatgttcac
    15121 cacgaagcct gctggtgcga caccaatcaa taccaacata accagaccaa tgcctttctg
    15181 accatcgttc gcgccatgcg aaaacgccac gccgatagcg gaaaggatca gcgcaatacg
    15241 cgtccagaac ggcggctttt tcttgccgtc tttcttttca cgctccgctg gggtcaggtg
    15301 gatacgggcg cgtttcttgg tgccgctcca gtagcgacgc agcaagaaaa tcagaccgcc
    15361 agcaaacacc aggccgacaa taggggaaac gatcagagaa ccgaaaatac ttaatacttt
    15421 cgggatattg agtgcatcca ccactgacgt cccggtcatc aacgcattgg ttaaaccaat
    15481 cccgatgatc gcgccaatca gcgtatgaga gctggatgca ggtaaaccaa agtaccaggt
    15541 acccaggttc cagataatcg ccgccagcaa catagagaac accatggcaa ggccatgaga
    15601 cgatcccata ttaagcagca gatccgtcgg cagcatatgc acaatggcat aggcaacact
    15661 cagaccaccc agcaaaacac ccaaaaagtt gaacaccgcc gccataacca cggcgagctg
    15721 agaacgcatc gcgcgggtat agataacggt tgccacggcg ttggctgtgt catggaaacc
    15781 attgatggct tcgtagaaca gcacaaaagc cagtgcaagc aataataaca gcccggtatg
    15841 caaatccagg ccagcaaaca aatgtagcat aggacgttac gccattttga ggacatgaac
    15901 gcggcgcatt atcagtgact ttcgcggcgc gggcaaagtg aaatatagac tttttttgat
    15961 ttgcctcctg tatggatttc actcaaaaaa taattatctt atataattca ggtaaatact
    16021 tccttttagt aatattgatg ctggtgcgac cactgaggaa tctttacaat tcacgcccgt
    16081 tttttcttag aggagcgcaa cgtggaaagg tttgatgcca ttattatagg cgctggtgcg
    16141 gcgggtatgt tctgttctgc gctggcaggt caggcaggac gccgggttct gctgatcgat
    16201 aatggtaaaa aaccagggcg caaaatcctc atgtctggcg gtgggcgctg caactttacc
    16261 aacctttatg tcgaaccggg cgcttatctg agccagaatc cgcatttttg taagtctgca
    16321 ctcgcgcgtt ttacccagtg ggatttcatt gatctggtca ataaacacgg catcacctgg
    16381 cacgagaaaa cgttagggca actcttctgc gatgactccg cgcagcagat tgtcgacatg
    16441 ctggtggatg agtgcgagaa gggcaatgtg accttcagat tgcgtagcga agtgctgagt
    16501 gtggcgaagg atgaaacagg cttcacgctt gaactgaacg gcatgactgt cggttgcgaa
    16561 aagctggtca tcgcgaccgg tggactgtca atgccagggc tgggcgcgtc gccgtttggt
    16621 tataagattg ccgaacaatt tggcctcaac gtgctgccga cccgcgcggg cctggtgcca
    16681 ttcactctgc ataaaccgtt gctcgaagag ttacaggtgc tggcgggcgt ggcggtgcct
    16741 tccgtgatta ccgctgaaaa cggcatcgtt ttccgtgaga acttactctt cacccaccgc
    16801 ggcttatctg gaccggcggt gttgcaaatt tccagctact ggcaaccggg tgaatttgtc
    16861 agtatcaatc tgctaccgga tgtggacctc gaaaccttcc tgaatgagca gcgtaatgca
    16921 catccgaatc agagcctgaa aaacacactg gcggttcatc taccgaagcg gttggttgaa
    16981 cgtttacaac aactcgggca aatcccggat gtttcgctaa aacagctcaa cgtgcgtgac
    17041 caacaggcac tgattagcac attgaccgac tggcgcgtac aacccaacgg cactgaaggc
    17101 tatcgcactg ccgaagtgac gctcggcggc gtggacacca acgaactctc ttcacggacg
    17161 atggaagcgc gcaaagtgcc tgggctgtac ttcatcggcg aagtgatgga cgtcaccggc
    17221 tggctggggg gctataactt ccagtgggcg tggtcgagtg catgggcttg tgcgcaggat
    17281 ttggtggcag aataagaagc aaaatgtgct ctcgatacca gagggattaa cctgaataaa
    17341 accagataag gcgtccaacg ccttatctga agcctttttt attatttttt cgctgaaccc
    17401 gcttctaata tagaaacgat ggaaaacagt gtaaaacaaa ctgcacctaa gccaaccaga
    17461 actcgcgaag gaataaagta agccatatcg gtttgcgcaa tttcagccag gaacgcagca
    17521 aggaacagac aaaacagaca ggtaaatacc ggaatcatgg gtattctgtt ggctaacgaa
    17581 caggtgcgtc gccataccag cgctaacaac cacactttgg agaaaatact gtagcagatc
    17641 atccccaggc aaatgagaat aatgccgggg gccaggcggg cgcttgcatc tgaactgatt
    17701 aatacgtaga taccaaaaat aatggtaagt gagccaagca aaataaccca atagcaccac
    17761 agccaatgtt ctttcactga gaacgtattc cgcgtttgat ggacaatagt ggcgacaagg
    17821 ccaataagac aagcgcaaat tgccgttaac cccattagta cgtgacctgc aacataatgt
    17881 ggtgtgatat ctgcactacg taacagtatg actgaccaaa tgaaaccaaa gagagtaagc
    17941 agaacgggaa cggcaattaa gcaattaccg attgttgaag aataggcctg caatggcgta
    18001 ccgtcactct tactgcccga ggcgtttttg gggatcaaca gaaaatgtcc ggaagatgcc
    18061 gctacggttg atacacaagc ggcaatcatg ccaacgccga aaataacatg accggcgaca
    18121 aattcatctg ccatcacatt attgcttgcc aacagtgacc atccccaaat catagttgta
    18181 gcagaaccgg cataaccaat aacgggaaat aacctgttgt agaatttatt catgccgtga
    18241 gttaactgag atataataat aaatgcggta gtgaacaatg ccagacatat cgcagaaaga
    18301 gaaatcagaa catgccctgc aacgaaatat tcactttgcc cagaaccggt taatacaaac
    18361 tcacctaatg cgatgcatat tatacccatc agtaaaggaa tta
//
LOCUS       DAPHCV010000098        18243 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.098, whole genome shotgun sequence.
ACCESSION   DAPHCV010000098 DAPHCV010000000
VERSION     DAPHCV010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.098"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(224..1252)
                     /gene="epmB"
                     /locus_tag="RH033_003502"
     CDS             complement(224..1252)
                     /gene="epmB"
                     /locus_tag="RH033_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HDV2992634.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIERMEKGNPDDPLLRQVLTSQEEYIVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFAHSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTQLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFVVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            1294..1860
                     /gene="efp"
                     /locus_tag="RH033_003503"
     CDS             1294..1860
                     /gene="efp"
                     /locus_tag="RH033_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDV2992635.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            1912..2037
                     /gene="ecnA"
                     /locus_tag="RH033_003504"
     CDS             1912..2037
                     /gene="ecnA"
                     /locus_tag="RH033_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HDV2992636.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            2148..2294
                     /gene="ecnB"
                     /locus_tag="RH033_003505"
     CDS             2148..2294
                     /gene="ecnB"
                     /locus_tag="RH033_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HDV2992637.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            2469..2786
                     /gene="sugE"
                     /locus_tag="RH033_003506"
     CDS             2469..2786
                     /gene="sugE"
                     /locus_tag="RH033_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDV2992638.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2783..3316)
                     /gene="blc"
                     /locus_tag="RH033_003507"
     CDS             complement(2783..3316)
                     /gene="blc"
                     /locus_tag="RH033_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HDV2992639.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3405..4538)
                     /locus_tag="RH033_003508"
     CDS             complement(3405..4538)
                     /locus_tag="RH033_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HDV2992640.1"
                     /translation="MFKMTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGI
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDDVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANASIGLFGALAVKPSGLSFEQAMQTRVFQPLKLTHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDITEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            complement(4601..4960)
                     /gene="frdD"
                     /locus_tag="RH033_003509"
     CDS             complement(4601..4960)
                     /gene="frdD"
                     /locus_tag="RH033_003509"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDV2992641.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(4971..5366)
                     /gene="frdC"
                     /locus_tag="RH033_003510"
     CDS             complement(4971..5366)
                     /gene="frdC"
                     /locus_tag="RH033_003510"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HDV2992642.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(5377..6111)
                     /gene="frdB"
                     /locus_tag="RH033_003511"
     CDS             complement(5377..6111)
                     /gene="frdB"
                     /locus_tag="RH033_003511"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HDV2992643.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(6104..7912)
                     /gene="frdA"
                     /locus_tag="RH033_003512"
     CDS             complement(6104..7912)
                     /gene="frdA"
                     /locus_tag="RH033_003512"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HDV2992644.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQAMERASTAGNGNDAAINAQVAGVEQRLKDLVNQDGGENWA
                     KIRDEMGMAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            8237..9214
                     /gene="epmA"
                     /locus_tag="RH033_003513"
     CDS             8237..9214
                     /gene="epmA"
                     /locus_tag="RH033_003513"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HDV2992645.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9433..10935
                     /gene="yjeM"
                     /locus_tag="RH033_003514"
     CDS             9433..10935
                     /gene="yjeM"
                     /locus_tag="RH033_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HDV2992646.1"
                     /translation="MPHSIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            10998..11312
                     /locus_tag="RH033_003515"
     CDS             10998..11312
                     /locus_tag="RH033_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992647.1"
                     /translation="MDDASRDPVITEDEIRELQFSAGDVAEIEQTVLSFVDTRHTRKV
                     AMVVGNTINTLKERDGPRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPI
                     IS"
     gene            11309..11623
                     /locus_tag="RH033_003516"
     CDS             11309..11623
                     /locus_tag="RH033_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HDV2992648.1"
                     /translation="MSARMFILCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEDDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(11654..14977)
                     /gene="mscM"
                     /locus_tag="RH033_003517"
     CDS             complement(11654..14977)
                     /gene="mscM"
                     /locus_tag="RH033_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HDV2992649.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQISQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNSPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(14999..15967)
                     /gene="psd"
                     /locus_tag="RH033_003518"
     CDS             complement(14999..15967)
                     /gene="psd"
                     /locus_tag="RH033_003518"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDV2992650.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLDALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTEIFVTPDAEPSPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(16064..17116)
                     /gene="rsgA"
                     /locus_tag="RH033_003519"
     CDS             complement(16064..17116)
                     /gene="rsgA"
                     /locus_tag="RH033_003519"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HDV2992651.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMEFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            17211..17756
                     /gene="orn"
                     /locus_tag="RH033_003520"
     CDS             17211..17756
                     /gene="orn"
                     /locus_tag="RH033_003520"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HDV2992652.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            17967..18042
                     /locus_tag="RH033_003521"
     tRNA            17967..18042
                     /locus_tag="RH033_003521"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18000..18002,aa:Gly,seq:gcc)
BASE COUNT         4211 a         4677 c         4800 g         4555 t
ORIGIN      
        1 acatatcaaa cgaatattta aggctaatgc aacgggcaaa gattaaactt tccagtttaa
       61 tgcaggttgc tgtactcgtg ttcgttgctc ttgccggatg cggcgtaaac gccttatctg
      121 gcctacagtt catagaaaat tcattcaatt tcaaagcacg atgtaggccg gataagcgta
      181 gcgcatcagg catttttaat gtttaacttc cctgtttaat cagttactgc tggcgtagct
      241 ggagatccag cggagtcttg ctgggttcgc caccaatttc gcgcgccagt tttggcacca
      301 gatatcccga tactaacgtc aacaactcac gcataatctg ccgtgcttca tcatcgctca
      361 ccacgaaatg cgccgcgccc tgcactttat cgagcacgtg caggtaatag ggcatcacac
      421 cggcgtcaaa cagtgcatta ctcaaatttg ccagcgtttg cgcgttatcg ttcacgccac
      481 gtaacagaac gctctggtta agctgggtga cgccgacacg gcgcaattta gccatcgcct
      541 ggcggaatgt ttcatctacc tcattggcat ggttgatgtg attcaccagc aagatttgca
      601 gcgtagaatg agcaaaacgt tcaaccagcg catctgtgat gcgtgccgga atcacaatcg
      661 gcagacggct gtgaatccgc agacgtttga tatgcgggat ggcttccagt tgtgtgagca
      721 accagtccag ctcgtgatct ttcgccatca gcggatcgcc gccggagaaa atcatctcgt
      781 ccagttccgg atgcgcagca acatactcaa gtgccgtctg ccagttacgt ttattgccct
      841 gattttcggc atacgggaag tggcggcgga agcaatagcg gcaatttacc gcacagccgc
      901 cttttaccag caatagcgct cggttgtggt atttatgcaa caaaccaggc accacgctgt
      961 gctgttcttc cagcggatcg gtggagaatc ccggcgctac aatatactcc tcttgcgatg
     1021 taagcacctg acgcaaaaga gggtcatcag gattaccttt ctccatgcgt tcaataaatg
     1081 atcgaggcac acgcagggca aacagctttt tagcactgcg tccggccaac agtttttcgt
     1141 ccgcgtctat attcaaaaga cgcagaagtt catcaggatc ggtcacaaca tcggcaagtt
     1201 gcgttaacca atcttctctg gatggggtat ttagggttac aatatgcgcc attttgtggc
     1261 ttagctacca attaacaaat ttcagagggc cttatggcaa cgtactatag caacgatttt
     1321 cgtgctggtc ttaaaatcat gttagacggc gaaccttacg cggttgaagc gagtgaattc
     1381 gtaaaaccgg gtaaaggcca ggcatttgct cgcgttaaac tgcgtcgtct gctgaccggt
     1441 actcgcgtag aaaaaacctt caaatctact gattccgctg aaggcgctga tgttgtcgat
     1501 atgaacctga cttacctgta caacgacggt gagttctggc acttcatgaa caacgaaact
     1561 ttcgagcaac tgtctgctga tgcaaaagca attggcgaca acgctaaatg gttgctggat
     1621 caggcagagt gtatcgtaac tctgtggaat ggtcagccga tctccgttac tccgccgaac
     1681 ttcgttgaac tggaaatcgt tgataccgat ccgggcctga aaggtgatac cgcaggtact
     1741 ggcggcaaac cggctaccct gtctactggc gctgtggtta aagttccgct gttcgtgcag
     1801 atcggcgaag tcatcaaagt ggatacccgc tctggtgaat atgtctctcg cgtgaagtaa
     1861 tacggttgtg gtgcggcctg caggctgcac catcacttat tctggtcaga gatgatgaaa
     1921 cgccttatcg ttcttgtttt gcttgccagc acgctgctca cgggctgtaa caccgctcgc
     1981 ggtttcggcg aagacatcaa acatctcggc aactccattt cccgcgctgc cagctaattt
     2041 ttcttctctt ccgaaaaatc atcagatttc cgtcattttt ggcgatgttg tctattatta
     2101 atttgctata ggcaaacata aataacatta cctaaaagga agacgttatg gtgaagaaga
     2161 caattgcagc gatcttttct gttctggtgc tttcaacagt attaactgcc tgcaacacca
     2221 cgcgtggcgt tggtgaagat atctctgatg gcggtaacgc gatttctggt gcagcaacaa
     2281 aagcacagca ataagcaata acggtacgac agctgtgtcg tgccgtttgt ttttttgcga
     2341 tagtcactaa ggtaatagtt gaaattcccc tgccacctgg caaaataccc gttcaaccat
     2401 cagctttgca ggacgacctg caaacgcctc ttttcaccgg ggacggcccc aattctccgg
     2461 agcctgatat gtcctggatt atcttagtta ttgctggcct gctggaagtg gtatgggccg
     2521 ttggcctgaa atatacccac ggttttagtc gtttgacgcc aagtgttatt actgtgacgg
     2581 cgatgattgt cagtatggcg ctacttgcct gggcgatgaa atcgttacca gtagggacgg
     2641 cttatgccgt gtggacgggt attggcgcag tcggcgcggc catcaccggt attgtgctgc
     2701 tcggtgagtc cgctaatccg atgcgcctgg cgagtctggc gttaatcgta ttggggatta
     2761 ttggtctgaa actcagcact cactaactac cgggctgctg tacccaaata aacttactga
     2821 catcaaaccc ttcccgggtc gcgactgcca gcatctcctg tttcacttcg tcagaaatgg
     2881 ttggcgtgcg ggagagtatc cacaggtagt cgcggtccgg gccgcaaacc agcgcatggc
     2941 ggtattcccg atcgagtgca ataacgttat aaccgccata gaaaggacca aagaatgaca
     3001 ctttcagcgc agcgcgagtt ggtgctccgg taaagtacgc tttcccttca ctctgctgcc
     3061 acatttctct gtcagggtta tagcctttat taatgacatt caggccgccg tcatcacgca
     3121 ggctgtatgt tgcggtgact ttttccagtc cacgttcaaa gcggtgatca aaacgggcaa
     3181 tctcatacca ggtaccgaga taacgtttag cgtcgaaatt attcactacg gtcacgccac
     3241 gcggcggcgt aggggaactg caggcaacga ccagaaatgc agctgtcgct gcggcaacga
     3301 gagggagcag gcgcataaat gtttccttac tggttttttt ctaagtgtag atgacagcaa
     3361 ggaaaagcgg agaaaaggtc caaaaattcg gacccgatga aattttactg tagagcgttg
     3421 agaatctgac aggcggcggc gactctcgct ggattgggat agtttttgtt agccagcatc
     3481 acgataccca gctctttttc tggaataaac gcgacatagc taccaaatcc gccggtcgcc
     3541 cccgttttat gtacccatga tgcgcgtact gcaggagttg ggggcgtaat cgcttttacg
     3601 gggcgtgctg ccagtgcaat tttattgtca ctgccgttaa tgatgatgtc aggattgacc
     3661 ggccagtcca gcatttccca gcccaggccc tgatacatat cgccggtttg ccagtagcga
     3721 gattgtgcca gttgtatccc ttgttgaagc gttttctcag tgatatcaag gggttttaaa
     3781 ttgctttgca cccagcgggc catatcttca atggtcgact tcacaccata agcctcagca
     3841 tctaacgccc ctggcgaaac atgcactgcc ttaccttcgc gatatcccca ggcgtaattc
     3901 ttttcttctg cggacggcac attaatccac gtatgagtga gtttgagtgg ctggaaaaca
     3961 cgagtttgca tcgcctgttc aaagctcaaa ccggatggtt tcaccgccag tgcaccgaac
     4021 aacccaatac tggcgttggc atacagacgt tgtgttcctg gagcccatgc aggttgccag
     4081 ttttgataga agcgcagcaa gtcgcttgag gatttaacgt catccggcac ctgcaatggc
     4141 aggccgccag cggtgtaggt cgcgagatgt aatagtgtga tcccattcca ttgtttagcg
     4201 gtaagttcag gccagtattt tgtcgcggga tcgcttaact tgatttcccc tcgagcaata
     4261 gcgtcgccac caagcacgcc cgtaaatgtt ttgctgaccg aacctaactc aaacaacgtt
     4321 tgctgtgtga cgggctgctt tttggcgatg tccgcatagc cccaggtaaa gtaataaggt
     4381 ttaccctgat aaattaccgc caccgctata ccggggatct tttgttgctc tataagcggg
     4441 gtaattgtgc gatgcacaat atcgttgatt tgttgagggg cagcaaatgt ggagcaagag
     4501 gcggtaatta ataaggcgca gagcgtcatt ttgaacatag ggtctggttt ccatacaaaa
     4561 cggcccgcca taggcgggcc ggatttacat tggcgatgcg ttagattgta acgacaccaa
     4621 tcagcgtgac aactgtcagg atagcagcca gaccgtagaa aacccatttg cccgcaggta
     4681 cgtggatttt cagatcgtgc atcgcgtggt gcatacggtg taaaccacac cacagcggca
     4741 gaacgatcat caggaacagg aatacgcgac caatgaagct ctgcgcgaac gccagaacgc
     4801 gctcgtagct cagtgcgtca cccggaaaca gccccagtgg cagcagaata ccaaccagca
     4861 ggatcatcac cggcgcaata attgcgctcc acataccacc ggccccgaag aggccccaga
     4921 ataccggttc gtcagaacgc tttggatttg gattaatcat ctcaggctcc ttaccagtac
     4981 agggcaacaa acaggattac gatggtggca accacagtta ccgcccagag gcttttgata
     5041 attggctctg gtcccatttt ttcgtctttt acaatgatat tggccgcttt tggtgccagc
     5101 tcaaaccagg ttttggtgtg cagcagggct gccgccagag tgatcaggtt aatgatcacg
     5161 ataaccgggt tttgtaaaaa gtcgacgaat cccgcccagg cttccgggcc atttttcagg
     5221 gcaaacagcc cgaaaatcag ttcaatgctg aaccacacag ccggaaccgc cgtgccttcg
     5281 cgcagcatgt agaagcgata aaacggcaat tttttccacc aggtggacgt cattggccgt
     5341 acatacggtt tacgtttagt cgtcatgttg cactccttag cgtggtttca gggtcgcgat
     5401 aagaaagtct ttcgaacttt ctactttgcc ctgctgaatg gccgcagccg gatcgacgtg
     5461 tttcgggcag acttcggagc agtagcccac gaaagtacag ctccatacgc cgttctggct
     5521 gttcaactgc gccatacgct ccttcttacc gtggtcgcgg ctatcttcgt tataacgatg
     5581 cgccagcgta atggcagccg gaccgatgaa ctctgggttc aggccaaact gcgggcacgc
     5641 ggcgtagcac agaccacagt tgatgcaacc ggagaactgg tgatacttcg ccatctgcgc
     5701 cggggtctgg atgttagtac cctgatccgc ggtgcgggag ttgccgatga tgtacggttt
     5761 gatcgcttcc agactttcga tgaagtgggt catatcgacc accagatcgc gttcaatcgg
     5821 gaagttagct aacgcttcaa ccttcatacc gtcggtgtaa tcacgcagga aggttttaca
     5881 tgccagtttt ggcacgttgt taaccatcat gccgcaggag ccacaaatcg ccatacggca
     5941 ggaccagcgg tagctcaggt ccggtgccag gttgtctttg atgtagccca gcgcatccag
     6001 taatgaggta gttgcgtcat aaggcacttc atagaatgcg ctatgcggtg cggtatcgac
     6061 ttccgggtta tagcgcacca cctcaatttt caggtttttc atctcagcca ttcgccttct
     6121 ccttcttatt ggctgcttcc gccttatcgg ctgcatccgc ttcgccaccg taaacacgtt
     6181 tagctggcgg cagcgtagta atcttcacgt cgctgtactc caggcgagtt gtgccatcag
     6241 catcgcggaa ggcgagggtg tgtttgagga agttgacgtc gtcacgctcg gtgcaacctt
     6301 cgtccagacg ctggtgcgcg ccgcgggact ctttacgtgc cattgcggag tgcgccatac
     6361 attcagcaac gttcagaccg tggcccagct caatggtgta gagcaggtcg gtgttgaaca
     6421 cgctggaagt gtcggtgatg cgcacgcgct tgaagcgttc ctgcagctct gccagcttgt
     6481 cgatggtttt ctgcatcagt tccggcgtac ggtagatacc gcagccttct tccatggcca
     6541 tgcccatttc gtcgcggatc ttagcccagt tttcgccgcc atcctggtta accagatctt
     6601 tcaggcgctg ttcaacgcca gcaacctgag cattgattgc tgcatcgttg ccattaccgg
     6661 cagtagatgc gcgttccata gcttgctcac cggccagacg accgaagacc accagttccg
     6721 ccagcgagtt ggagcccaga cggttcgcac cgtgcagacc aacagaggaa cattcaccca
     6781 cggcgaacag acctttaatg cgggtttcac agttctgatc ggtttcgata ccgcccatgg
     6841 tgtagtgtgc ggtcggacgt accggaatcg gttctttaac cggatcgacg ccaacgtacg
     6901 ctttcgccag ttcgcagatg aacggcagac gttcatgcag ttttttctcg ccgaggtgac
     6961 gcaggtcgag ataaaccaca tcgccacgtg gcgtggagat ggtgttgcct ttacgccatt
     7021 cgtgccagaa ggcctgagaa actttgtcgc gtggacccag ttccatatat ttgtttttcg
     7081 gctcgcccag cggagtttcc gggcccatgc cgtaatcttg cagataacgg tagccatttt
     7141 tgttgaccag aataccacct tcaccgcggc agccttctgt catcaggata ccggaaccgg
     7201 gcagaccggt tgggtgatac tgaacgaatt ccatgtcacg cagcggaacg ccgtggctta
     7261 gcgccatacc cataccgtca ccggtaacga tgccgccgtt ggtgttgtaa cgataaacgc
     7321 gacccgcacc gccggtagcc ataacgaccg cgttagcacg gatctgcacc agcgtgcctt
     7381 ccatcatgtt cattgctacc aggccgcgaa catgaccatc atcaaccaga atatccagca
     7441 cgaaatgttc gtcaaaacgc tggatctgcg ggaattgcag agaggtctgg aacagcgtgt
     7501 gcagcatatg gaagccggtc ttatcggcgg cgaaccaggt gcgctcgatt ttcatgccgc
     7561 cgaagcgacg tacgttgacg ctaccatccg ggcgacggct ccatgggcac ccccacagtt
     7621 ccagttgggt catttcggtt gggcagtggt ggacgaaata atccacgaca tcctgctcac
     7681 acaaccagtc gccacccgct actgtatcgt gaaagtgata ttcgaagctg tcatgatcct
     7741 gcgcgacagc ggcggagccc ccttctgcag caacggtatg gctacgcatc gggtatactt
     7801 ttgagattag tgcgattttt gcatttggat ttgcctgcgc ggcagcaatt gcagcacgta
     7861 atcccgcgcc accggcgcct acaatggcaa gatcggcttg aaaggtttgc acgacattcc
     7921 tccagattgt ttttatccca cagccccgta cttcagggta agtgcctgaa agttacggtc
     7981 tgcgaacgct attccactgc tcctttatag gtacaacagt atagtctgat ggataagtct
     8041 gaaatttgac gagatcgatt tttttagtgc gcaagagggc taaattatca ctgaagatga
     8101 ttaatttaat tactaaacca tcagatcgtg ctttttttag tcactgccca cccttgtttg
     8161 attcctgcgt aaaatttgtc tcgccgtcgc gttgcgagta gacttcgtgc ccttgtcaaa
     8221 aactggagat ttaactatga gcgaaacggc atcctggcag ccgagcgcat ccattcctaa
     8281 cttattaaaa cgcgcggcga ttatggcgga gatccgtcgt ttctttgccg atcgtggagt
     8341 gctggaggtg gagacgcctt gtatgagcca ggcgacggta accgatattc atttggtccc
     8401 gtttgagaca cgtttcgtag gccccgggca ttcgcagggg atgaatctct ggttaatgac
     8461 cagcccggaa taccatatga aacgcctgct ggttgctggt tgtgggccgg tattccagct
     8521 gtgccgcagc ttccgtaatg aagagatggg gcgttatcac aaccctgagt tcactatgct
     8581 tgagtggtat cgaccgcact atgatatgta ccggttgatg aacgaggtgg acgatctctt
     8641 gcaacaggtg ctggactgtc cggcagcaga aagcctttct tatcaacaag ctttcttgcg
     8701 ttatctggaa attgacccac tctctgccga caaaacgcaa ctgcgggaag tggcagcgaa
     8761 actggatttg agcaacgttg ccgatactga agaagaccgc gacacgcttc tacaattgct
     8821 gtttaccttt ggcgtagagc caaatattgg caaagaaaaa ccgacctttg tgtaccactt
     8881 tccagccagc caggcatcac tggcgcaaat cagtaccgaa gatcatcggg tcgctgaacg
     8941 ctttgaggtt tattataaag gtattgagct ggcgaatggt ttccatgaat tgacggatgc
     9001 ccgtgagcag caacaacgct ttgaacaaga taaccgtaag cgcgcggcgc gcggtttgcc
     9061 gcagcacccc attgaccaga atctgattga agccttgaaa gtcggtatgc ctgactgttc
     9121 cggcgtggca ttaggcgttg atcgtctggt gatgttggcg ctgggcgcgg agacactggc
     9181 tgaagtcatc gcctttagcg ttgaccgggc ataattctga aaaattactg aattaaccgc
     9241 gaagaatggc gtgataagcg ccattctgta gcatatgttt tcatttttcc gctaaaggcc
     9301 acaatttaag actggatatt tgatatcatc caggtatcaa ttctgtattg gtttttacgc
     9361 tgcccgctca atgttgggca gtgcagttca gtgggcgcgt tttatacacg cgctgaaatg
     9421 aaggatggtt tcatgcctca ctcgataaaa aagatgagtc tgataggact catattgatg
     9481 atctttactt ccgtatttgg atttgccaat agcccatcgg cttattactt aatgggttat
     9541 agtgcgattc ccttttatat attttctgca ttgttattct ttattccatt cgccttaatg
     9601 atggctgaaa tgggagctgc ttatcgcaaa gaagaaggcg gtatctattc ctggatgaat
     9661 aatagtgtcg gaccacgttt tgccttcatt ggtacgttta tgtggttttc ctcttatatc
     9721 atctggatgg tgagtacctc cgcgaaagtt tgggtaccgt tctcaacatt cctctatggt
     9781 agcgacatga cccagcactg gcgtattgcc ggactggagc ctacgcaggt ggttggtctg
     9841 ctggcagtgg catggatgat tctggtcacc gtcgttgctt caaaagggat taataaaatt
     9901 gcccgcatta ctgcggtggg cggtattgca gtaatgtgtc tgaatttagt attgctgtta
     9961 gtaagcatta ctattttgtt attaaatggt gggcatttcg cgcaggatat taatttcctt
    10021 gcatcaccga acccaggtta tcagtccggt ctggcaatgc tatcgtttgt ggtatttgct
    10081 atttttgcct atggtggaat tgaagcggtt ggtggtctgg tcgataaaac ggaaaatcca
    10141 gaaaagaact ttgccaaagg tattgttttt gccgctattg ttatttcaat cggttattcg
    10201 ctggcaatat ttttatgggg cgtcagcaca aactggcagc aggtattaag taatggttcc
    10261 gttaacctcg gcaatattac ctatgtgctg atgaagagcc tcggggtgac gctgggtaac
    10321 gcactgcatt tgtcacctga agcgtcattg tcgctgggcg tatggtttgc gcgtattacc
    10381 ggactttcga tgttcctcgc ttataccggt gcgttcttta cgctttgcta ttcaccgctg
    10441 aaagccatca tccaggggac gccgaaagcg ttgtggccgg aaccgatgac gcgcctgaat
    10501 gcgatgggga tgccgtctat cgccatgtgg atgcagtgcg ggttggttac tgtcttcatc
    10561 ctgctggttt cgtttggtgg cggtaccgca tcggcgttct ttaacaagct gacgctgatg
    10621 gcgaacgtgt ctatgacgct tccttacctg ttcctcgcgc tggctttccc attctttaaa
    10681 gcacgtcagg atctcgacag accgtttgtg attttcaaaa cgcatatgtc ggcaatgatt
    10741 gcaacagtgg ttgtcgtact ggtggtgaca tttgcgaacg tcttcaccat cattcaacct
    10801 gtggttgaag ctggagactg ggacagcaca ttgtggatga ttggcggccc tgtcttcttc
    10861 tcgctgttag cgatggcgat ttaccagaac tattgcagtc gcatggcgaa caaacctgag
    10921 ttagctctcg actgatcata tgaaatcctg caactcatcg tctctccggc gatgagttgt
    10981 ctggaaagga agacgttatg gacgacgcta gtcgggatcc agtgataacc gaagatgaaa
    11041 tcagggagct gcaattttct gccggggatg tggcggaaat agagcaaacg gtcctctctt
    11101 ttgtcgatac ccgccacacc cgcaaagtag cgatggtggt gggtaacact attaataccc
    11161 tcaaagagcg cgatggtccg cggtggggaa acctgccgga tatctattgc gcttatctta
    11221 ttagatgtct ggtttttcgc ggagaattag tgggatacgg cgatttgttt cgtatgcgct
    11281 atagcgaaat taagcgaccg attatctcat gagtgcgcgc atgtttatct tgtgctgcat
    11341 ctggtttatt gtggcgttcc tctggataac catcacttcc gcgctggaca aagagtggat
    11401 gattgatgga cgcgggatta ataacgtctg cgatgtgctg atgtatctcg aagatgacga
    11461 tacccgtgat gtcggtgtga taatgacgtt accgctcttt ttcccttttc tctggttcgc
    11521 tttgtggcga aaaaaacgcg gctggtttat gtacgccacc gcgctggcta ttttcggcta
    11581 ttggctgtgg cagttttttc tgcgctatca gttttgctta taagccggat gggttcatcc
    11641 ggccacaaag accttacaaa cttcccgcct gacgaccttt gcccgcagaa gttaatgttc
    11701 tccccgtttg tttaccattc aggctttcca gacgcatctg gaagggcggg aatggcatat
    11761 cgataccgtg ggcatggaat cctgccagaa ttagctggtg aatctcatgg cgtagtggca
    11821 tacggtgacc catctcagcg gcgtaaatac gcagctcgaa aatttgaatc ccctgttgca
    11881 gatccaccag gaagacttcc ggcgccgggt tgtcgatcac cagcgagcag cgacgcgctg
    11941 cggtgagcag gatttccgtc acttcttcac tgttggcatc ggcaggggcc ggaatcgtca
    12001 acaccacgcg cgtgaccgag tcagagagcg accagttgat aaactgctcg gtaataaacg
    12061 ccttgttcgg cacgataatt tctttgcggt cccagtcgct gatggtggtg gcgcgggtgt
    12121 taattttcgt cacactaccg gtaagatcgc ggatcgttac cgtatcgcca atgcgaatcg
    12181 gtttttcgaa caggataatc aggccagaga taaagttggc gaaaatttcc tgcaaaccaa
    12241 aaccgagacc aacaccgagc gcggcgacca gccactgcaa tttcgaccac tcaataccaa
    12301 tcatcgagaa gccgaccagc ccgccaatca gcatcagcag atatttggtg atggtggtga
    12361 tggcgtagcc cgtacccggc gttaaatcca ggtgctgcag aatcgccagt tccagcagcg
    12421 cgggcaggtt gcgcaccagc tgcgtggtga tgataaacac cagaatggca atcagcaccg
    12481 caccgagggt aatcggctcc aggctttcta cgccctgtac cgtggaagtg acatcccaca
    12541 gcgaaatatt ttcgaggaag ccgaaagcgg aatggatttc tgaccacagc acaatgaccg
    12601 aaagcagggc gatcaacatc aaaattgagc gcaccagccg caaggattgc gcactgatgg
    12661 catcgagatc gacttcgctt tcatcgactt caattgctcc ttccgggcta ctgtgatgat
    12721 gcgcttcctc ttcgccacgc gcacgctgcg ctaacatctc tgcccggcga tgcttcgccc
    12781 ggtcaaacgc cagcctgcgg cgctggatca gcatccagcg gcggataacg tgataaacca
    12841 ccagtagcaa gaaccagatg gcaaccgagg tttcaagcct cgctaacagc gcctgcgccg
    12901 ttgccagata acccaccgcc gacgccagaa tggcaaccag cggtgcgcca atcatcatgt
    12961 tccacagcat atggttggta atgttgtcgc cgctgccttc tttgttgaga tacagcggga
    13021 tcccggcctt tttcagactg agagtgacca ccgccagcgc accgcaaatg aggataaagc
    13081 aaagccgtcc cagcgaaccg gagaattcac ggtcgtcgag gttatcgaac atcatcagcg
    13141 ccataatcag cggcacaata agcccgatgc tcatcaggta gtagcgcatc ccacgggaaa
    13201 cccgttcgcg cggccagcca aaatgagcga taaacaagcc gttcgggcgg gcaaaggtgg
    13261 cgcaaatcat cactacccac agcagcggca cggtggctgt tacgccatca ccaatcgcga
    13321 ccgccagcgg ataaggccac gcctcgcgca agccgtaacc cagcgtcatc cacagcacag
    13381 gcagcggtga cgcgacgaga atcgaccaga aaagagtgcg taacgtcagc cagaagtgat
    13441 cctgagtcac tttgccgact ttcgccgccg aacgttcaag aaaacgggtg aaatagcggc
    13501 gcgagtaaat actgcaaccg accagaatca acgcgccaaa cagcggcaaa atcgtctctt
    13561 tactggtcag catcattaca ctggctttgc ccaactgact gaaggtgtcc agcgaaatga
    13621 gacgacgcag atcctgggcg atttccagcg gccaggcgat ggtcatcggg cgcacgtcag
    13681 aggtccagaa cagatagcgg tgcgtcgctt cattcacctc tttcagcgca tcctccagtt
    13741 gcccgttgga gactttcagc ttggtcagtt ccagtagtag cgtgtctcca ccctgcaaca
    13801 atgagttcag caactcacgc tgagtgcgta gctgtgcttc cagaatacgg ttttgctcgg
    13861 cggtcagcgg ctgaccgtcg gcctgatgga tttgccgtag cagcggctgt ttatttagca
    13921 gatcctcata acgtaaccgt tgcacacgca actgcgccat ttcggtatca agctgttgtg
    13981 gttttggcat ttccggtagc cgtgccacct gcgcccgcaa cgcttcaccg agcaggttgg
    14041 acgatcccag ccattgcgac tgttcacgca gcgtattcaa cgcctgccgg acctgtaacg
    14101 tctggctggc agcctgacgc tgttgcgagg caacgagatc catccgctgc gcctgttgat
    14161 tcagcgctgc cgatagttcg cggttaattt tgaattgcgc gacgatatct ttcggcaaat
    14221 cggcactgtt ttctgccagt aattcggtac tttccagcgc ccgctccgcc tcaagctgac
    14281 gttggctgtt caactgatta cgtaaggcct gcaaatacgc atccagttgc tggctctctt
    14341 tttccgccag ctctgagcgt aagcgcgcta attcctggcg gttattggcg gacagctgcg
    14401 cgagttccag ttcatcgacg agcgctttca ggcgtgcgga gtcagactgc aacgcgaaat
    14461 tttgtgcctg attgagcggg gaattgccgg taagcgtccc caggcggcgc tcgatttcat
    14521 ttaactgacg gcgggcatcg gtttgctgtt gcggcagttg attcagcgaa tcggcaatct
    14581 cgcgggcgcg ttcctgctct tgctgggcct gacggctttt atccagcaac tggctactga
    14641 cctggagaat ttcctgattc agcgcgtcgg tagacattcc cggtgacacg ctgcgcggct
    14701 cgtcacgcat gttgtttaat tgcgcgcgca gagtagcgga gagtttcgga taattatcaa
    14761 tgacttcttg atattgcttg atgcgctcaa gggaaccttt ccgttcctca agcgcattta
    14821 aggcagactg gagcgcctct acgacttccg gttgcgcggg tttcgccgct tttgcctgct
    14881 ccagttcttg tgagatttgt ttgctatcgg gggccgtcgc ggcgtacgcc ccccaactga
    14941 ggcaccaggc catcagaaaa gtgataatca ggcgcacgtc agcgtttcct ttgatggatt
    15001 agacctggtc ttttttgtcg tcaaccaatg ggctggcgtc gtgttctgct tcgatctctt
    15061 cagcaggaag cggggagggt tcagcgtctg gcgtaacaaa gatttcggta gatactgcca
    15121 gcggctggcc aattttcgta acagacaggc tttccagttg ctcaaccaga ttcactttac
    15181 ccggtgcaaa caggttgata acggtggaac cgagtttaaa gcgacccatt tcctggcctt
    15241 tcagcagtgc cacagaaccg tcgttttccc cggcaggcca ggtccagcgc ttgatgatac
    15301 cttcgcgcgg cggcgtaatg gtgcccgccc agaccgtctc aatgctgcct acaatcgtcg
    15361 ccccgaccag aatctgcgcc attgggccaa attcggtatc gaacaggcag atcacgcgtt
    15421 cgttacgggc aaacagattc ggcacgttct gagcggtgag atggttaacg gaaaagagat
    15481 cgcccggcac gtagatcatc tcacgcagaa taccgttgca cggcatgtgt acgcggtggt
    15541 agtcacgcgg ggagaggtaa gtggtcacaa acgtaccgtt gcggaacagg tccgccatca
    15601 gatagttgcc tgccagcagg gcgtcgaggc tgtagttgtg gcctttggct tgcaggattt
    15661 tatcttcttc gattttaccc aactggctga taacgccatc ggcaggcata accagtacat
    15721 tcggatcggt atcgattggg cgtacttcgt cacgcagcgg acggacaaag aattcgttaa
    15781 aggtgcggta gctggcggtg tccggctttt gcgcctcttt catatcgacc ttgtagtatt
    15841 taacgaacag atcgataacc agttttgtca gccatcctgc ccgcttgctt gcgccccaac
    15901 ccgccaggcg agtaagccat agtttcggca gaatgtactg tagcgaaagt ttaaatgaat
    15961 ttaacaaggt agcctccagg ccattgtttt gtcgttcctg atccggccta catgccggat
    16021 cctgaaaaaa aggggacgat tctaacgacg gttagcttaa ttgtcagtca tccgtatcag
    16081 aaaagttttt acgcgttttt acctgcgcca tgctttccag aatacggtga tagttttcga
    16141 aacgggtttc cgcgattttt ccttcctcaa ccgcttcccg gatagcgcag cccggatcgg
    16201 tatcgtgttt gcaatcgcga tatttacaca gacctaagta gtcatggaat tcgacaaagc
    16261 cctgagtgat ttgttccggc tccaggtgcc agaggccgaa ctcacgcact cctggggagt
    16321 caatcacatc accgccgtgc gggaagtgat acagccgagc ggcggtcgtg gtgtgctgac
    16381 cgagacccga gttgtcagag acatcgttgg tcaggatctc tttttgcaga cccagcaatg
    16441 cgttcagcag gctggatttg ccaacgccag actgcccggc aaaaatgctg atgcgcccgg
    16501 tcaacgcctc ttccagcggt ttcagcccat cctgagtatg actggaaacc atcaatacgc
    16561 gataaccgat attgcggtag atatccatct gctcgttgac gaactccatg ccttcgtcgt
    16621 ccagcaggtc tatcttgttg agcacaataa tcggctcaat ctgcaaggtt tcgcaggcca
    16681 ccaggtaacg gtcgataata ttgagcgaca gctccggcaa aatggcggag acaatgacaa
    16741 tctggtcgat gttggcagca ataggtttca caccgtcgta aaaatccggg cgcgtcaaca
    16801 ctgaggtgcg ctcatgcacc gcttccacga tccctttgac atttacgcct tccgccgctg
    16861 gtttacccgg acgccagact acgcggtcgc cggtcaccaa cgaacggatg gtacggcgaa
    16921 tattgcagcg gtgaacgtcg ccatcggcgg attccacatc agcgtgcata ccaaagcggc
    16981 tgatgacgat accttcatca ggctcgccaa acagattgtc gtcgtagtcg ggcttctcct
    17041 tagacgtttt aagacgacgc tggtgattgg cgttcacgcg gcgctgctgg cctttggaga
    17101 gtttattttt actcaatcgt acagactcct ggtcgcccct gatgggcaaa acatctatga
    17161 tacacgcaat tgtggatcaa tatagtcact gtgaatgggt ggaaaatagc atgagtgcca
    17221 atgaaaacaa cctgatttgg atcgatcttg agatgaccgg tctggatccc gagcgcgatc
    17281 gcattattga gattgccacg ctggtgaccg atgccaacct gaatattctg gcagaagggc
    17341 cgaccattgc agtacaccag tctgatgaac agctggcgct gatggatgac tggaacgtgc
    17401 gcacccatac cgccagcggg ctggtggagc gcgtgaaagc gagcacgatg ggcgatcgtg
    17461 aagctgaact ggcaacgctc gaattcttaa aacagtgggt gcctgcggga aaatcgccga
    17521 tttgcggtaa cagcatcggc caggatcgtc gcttcctgtt taaatatatg ccggagctgg
    17581 aagcctactt ccactaccgt tatctcgatg tcagcaccct gaaagagctg gcgcgccgct
    17641 ggaagccgga aattcttgat ggttttacca agcaggggac gcatcaggcg atggatgata
    17701 tccgtgagtc ggtggcagaa ctggcttact accgcgagca ttttatcaag ctgtaaaatt
    17761 tttcctggtc acgtaagcgc cggaatccac ttgcggcggc gtgattttga cgctaaattg
    17821 aacgtcttgc tgataatttc agcacttgag ataaaaactc aaaaaaaatt ttttaggggg
    17881 gttgcagagg gaaagatttc tcgtataatg cgcctcccgt aacgacgcag aaatgcgaaa
    17941 attacgaaag caaaattaag tagtacgcgg gaatagctca gttggtagag cacgaccttg
    18001 ccaaggtcgg ggtcgcgagt tcgagtctcg tttcccgctc caaaatttga aaagtgctgc
    18061 aaagcacaga ccacccaatc gcagtatttc gaattgcgac aaggcggcaa ctgaacgaat
    18121 ccttgggagc ttacaaaagt aagtgactaa ggtgagtgaa ggcagccaag gcagtagcgg
    18181 ttcgaaagac gaagattaag cgggaatagc tcagttggta gagcacgacc ttgccaaggt
    18241 cgg
//
LOCUS       DAPHCV010000099        17928 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.099, whole genome shotgun sequence.
ACCESSION   DAPHCV010000099 DAPHCV010000000
VERSION     DAPHCV010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.099"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            86..160
                     /gene="tnaC"
                     /locus_tag="RH033_003522"
     CDS             86..160
                     /gene="tnaC"
                     /locus_tag="RH033_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HDV2992653.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            381..1796
                     /gene="tnaA"
                     /locus_tag="RH033_003523"
     CDS             381..1796
                     /gene="tnaA"
                     /locus_tag="RH033_003523"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HDV2992654.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1888..3135
                     /gene="tnaB"
                     /locus_tag="RH033_003524"
     CDS             1888..3135
                     /gene="tnaB"
                     /locus_tag="RH033_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HDV2992655.1"
                     /translation="MTDQSEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHAGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3394..4569
                     /gene="mdtL"
                     /locus_tag="RH033_003525"
     CDS             3394..4569
                     /gene="mdtL"
                     /locus_tag="RH033_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HDV2992656.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMAIMGIAVLMLSLFILKETRPAAPTTSDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLATGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPIAAHEEIHHHA"
     gene            4544..5503
                     /gene="yidZ"
                     /locus_tag="RH033_003526"
     CDS             4544..5503
                     /gene="yidZ"
                     /locus_tag="RH033_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HDV2992657.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHEAWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSRNPKIVWLRETIKNLYASMA"
     gene            5660..6409
                     /locus_tag="RH033_003527"
     CDS             5660..6409
                     /locus_tag="RH033_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HDV2992658.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            6431..6997
                     /gene="yieF"
                     /locus_tag="RH033_003528"
                     /gene_synonym="chrR"
     CDS             6431..6997
                     /gene="yieF"
                     /locus_tag="RH033_003528"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HDV2992659.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEINALPSIA
                     DIPLYDADVQQEDGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(7051..8388)
                     /gene="adeP"
                     /locus_tag="RH033_003529"
     CDS             complement(7051..8388)
                     /gene="adeP"
                     /locus_tag="RH033_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HDV2992660.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMSVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDISPCVIIVALLFILKIVFIDAH"
     gene            8555..9220
                     /gene="yieH"
                     /locus_tag="RH033_003530"
     CDS             8555..9220
                     /gene="yieH"
                     /locus_tag="RH033_003530"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HDV2992661.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLEEIFT
                     RFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVARLFDTELADIAGANELLASINV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKQIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            9287..9760
                     /locus_tag="RH033_003531"
     CDS             9287..9760
                     /locus_tag="RH033_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HDV2992662.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLILLVIIQ
                     VLPLFLLMSITTGAIPAMLTGVMVACLPEKIGSQKRYRCLVGGIGGVVITEIYCAVIV
                     HIKDMASSALFENILSGENLVVRIIPALLAGVVMSRIITHLPGLDISCPETDSLS"
     gene            complement(9842..10564)
                     /locus_tag="RH033_003532"
     CDS             complement(9842..10564)
                     /locus_tag="RH033_003532"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDV2992663.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKMRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEESIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLIVIADKAAAAELALG"
     gene            complement(10579..11748)
                     /locus_tag="RH033_003533"
     CDS             complement(10579..11748)
                     /locus_tag="RH033_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDV2992664.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     TDNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPALKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVVHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGIGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSPGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNEPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(11775..13391)
                     /gene="bglH"
                     /locus_tag="RH033_003534"
     CDS             complement(11775..13391)
                     /gene="bglH"
                     /locus_tag="RH033_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HDV2992665.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYATNPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGCYVSANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKYNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(13477..14871)
                     /gene="bglB"
                     /locus_tag="RH033_003535"
     CDS             complement(13477..14871)
                     /gene="bglB"
                     /locus_tag="RH033_003535"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HDV2992666.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQVGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     GQIDPVGLRILLNTLWDRYQKPLFIVENGLGAKDSVEVDGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(14890..16764)
                     /gene="bglF"
                     /locus_tag="RH033_003536"
     CDS             complement(14890..16764)
                     /gene="bglF"
                     /locus_tag="RH033_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HDV2992667.1"
                     /translation="MTELARTIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDDKDNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWATEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKVDALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPITFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLIPLIINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPGIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMFH
                     FITAKSQSAQVAQQEKTPEVITPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIAI
                     LPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNVG
                     DKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQINAGEPLLSIIR
                     "
     gene            complement(16898..17734)
                     /gene="bglG"
                     /locus_tag="RH033_003537"
     CDS             complement(16898..17734)
                     /gene="bglG"
                     /locus_tag="RH033_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HDV2992668.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRPGERINSS
                     GIEKEYALSSHELNGRLSELLSHMPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT         4584 a         4495 c         4160 g         4689 t
ORIGIN      
        1 tcgattcaca tttaaacaat ttcagaatag acaaattcct tgagtattat actgtagcct
       61 cgtgtctttc gaggataagt gcattatgaa tatcttacat atatgtgtga cctcaaaatg
      121 gtttaatatt gacaacaaga ttgtcgatca ccgcccttga tttgcccttc tgtagccatc
      181 accagagcca aaccgattag attcaatgtg atctatttgt ttgctatatc ttaattttgc
      241 cttttacaaa ggccatctct cgtttattta cttgttttag taaatgatgg tgcttgcata
      301 tatatctggc gaattaatag atattgtaaa tgtaatattc acagggatca ctgtaattaa
      361 aataaatgaa ggattatgta atggaaaact ttaaacatct ccctgaaccg ttccgcattc
      421 gtgttattga gccagtaaaa cgtaccactc gcgcttatcg tgaagaggca attattaaat
      481 ccggtatgaa cccgttcctg ctggatagcg aagatgtgtt tatcgattta ctgaccgaca
      541 gcggcaccgg ggcggtgacg cagagtatgc aggccgcgat gatgcgtggc gacgaagcct
      601 acagcggcag ccgcagctac tacgcgttag ccgagtcagt gaaaaatatc tttggttatc
      661 aatacactat tccgactcac cagggccgtg gtgcagagca aatctatatt ccggtactga
      721 ttaaaaaacg cgagcaggaa aaaggcctgg atcgcagcaa aatggtggcg ttctctaact
      781 atttctttga taccactcag ggccatagcc agattaacgg ctgtaccgtg cgtaacgtct
      841 atatcaaaga agccttcgat actggcgtgc gttacgactt taaaggcaac tttgaccttg
      901 agggattaga acgcggtatt gaagaagttg gcccgaataa cgtgccgtat atcgttgcaa
      961 ccatcaccag taactccgca ggtggtcagc cggtttcact ggcaaactta aaagcgatgt
     1021 acagcatcgc gaagaaatac gatattccgg tggtcatgga ctccgcacgc tttgccgaaa
     1081 acgcctattt catcaagcag cgtgaagcag aatacaaaga ctggaccatc gagcagatca
     1141 cccgcgaaac ctacaaatat gccgatatgc tggcgatgtc cgccaagaaa gatgcgatgg
     1201 tgccgatggg cggcctgctg tgcatgaaag acgacagctt ctttgatgtg tataccgaat
     1261 gcagaaccct ttgcgtggtg caggaaggct tcccgacata tggcggcctg gaaggcggcg
     1321 ctatggagcg tctggcggta ggtctgtatg acggcatgaa tctggactgg ctggcttatc
     1381 gtatcgcgca ggtgcagtat ctggtcgatg gtctggaaga gattggcgtt gtctgtcagc
     1441 aggcgggcgg tcacgcggca ttcgttgatg caggtaaact gttgccgcat atcccggcag
     1501 atcagttccc ggcacaggcg ctggcctgcg agctgtataa agtcgccggt atccgtgcgg
     1561 tagaaattgg ctctttcctg ttaggccgcg atccgaaaac cggtaaacaa ctgccatgcc
     1621 cggctgaact gctgcgttta accattccgc gcgcaacata tactcaaaca catatggact
     1681 tcattattga agcctttaaa catgtgaaag agaacgcggc gaatattaaa gggttaacct
     1741 ttacctacga accgaaagta ttgcgtcact tcaccgcaaa actgaaagaa gtttaattaa
     1801 aacactcaga gtggctataa ggatgttagc cactctatta ccctacatcc tcaataacaa
     1861 aaatagcctt cctctaaagg tggcatcatg actgatcaat cagaaaaaaa acactctgca
     1921 ttttggggtg ttatggttat agccggtaca gtaattggcg gtgggatgtt tgctttacct
     1981 gttgaccttg ccggagcctg gtttttttgg ggcgctttta ttcttattat cgcctggttt
     2041 tcaatgttac attcagggct attattatta gaagcaaact taaattatcc cgtcggatcc
     2101 agttttaaca ccatcaccaa agatttaatc ggtaacacct ggaacattat cagcggtatt
     2161 accgttgcct tcgttctcta catcctcact tatgcctata tctctgctaa tggtgcgatc
     2221 attagtgaaa cgatatcaat gaatttgggt tatcatgcta atccacgtat tgtcgggatc
     2281 tgcactgcca ttttcgttgc cagcgtattg tggataagtt cgttagccgc cagtcgtatt
     2341 acctcattgt tccttgggct gaagattatc tcctttgtaa ttgtttttgg ttctttcttc
     2401 ttccaggtcg attactccat tctgcgcgat gccaccagca ccacggcggg aacatcttat
     2461 ttcccgtata tctttatggc tttgccggtg tgtctggcgt catttggttt ccacggcaat
     2521 attcccagcc tgattatttg ctatggcaaa cgcaaagata agctaatcaa aagcgtggtc
     2581 ttcggttcgc tgctggcgct ggtgatttat ctcttctggc tctattgcac gatggggaat
     2641 atcccgcgag aaagctttaa ggcgataatc tcctcaggcg gcaacgttga ttcgctggtg
     2701 aaatcgttcc tcggcaccaa acagcatggc attatcgagt tttgcctgct ggtgttctcc
     2761 aacttagctg ttgccagttc gttctttggt gtcacgctgg gattgttcga ttatctggcg
     2821 gatctgttta agattgataa ctcccacgcc gggcgtttca aaaccgtgct gttaaccttc
     2881 ctgccacctg cgttgttgta tctgatcttc ccgaacggct tcatttacgg gatcggcggt
     2941 gccggactgt gtgccaccat ctgggcggtc attatccccg cggtgctggc aatcaaagct
     3001 cgcaagaagt ttcccaatca gatgttcacg gtctggggcg gcaatcttat tccggcgatt
     3061 gtcattctct ttggtataac cgtaatttta tgctggttcg gtaacgtctt taacgtgtta
     3121 cctaaatttg gctaaacagc cacttatact ctaaataatt cgagttgcag gaaggcgaca
     3181 agtgagtgaa tccccaggag cttactaaag taagtgactg gggtgaacga acgcagacgc
     3241 agcacatgca acttgaagta tgacgagtat agaagccagc cattcgctgg cttcttgcct
     3301 ctcaggaaat cacttatgtc caaatggcaa ctcgcctgat cctccttcac cacgtatgct
     3361 ttgcgtcacc ttactatcag gacgctttag cccatgtccc gctttttgat ttgtagtttt
     3421 gccctggttt tactttatcc cgccgggatt gatatgtacc tcgttggttt accgcgcatc
     3481 gccgccgatc tcaatgccag cgaagcgcag ttgcatattg cgttctccgt ttatctggcg
     3541 ggcatggcag ctgcgatgtt atttgccggt aaagtggccg atcgttcagg gagaaagcct
     3601 gtcgccatac ccggcgcggc actatttatt atcgcctcgg tgttctgttc actggctgaa
     3661 accagcacgt tatttcttgc agggcgcttt ctacaggggc tgggcgcggg ctgttgttac
     3721 gtggttgcgt tcgccatttt acgcgacaca ctggatgatc gacgtcgggc taaagtgctg
     3781 tcgttactca acggcattac ctgcatcatt ccggtgttag cgccggtgct cggacacctg
     3841 attatgctta aattcccatg gcagagtctg ttctggacaa tggcaataat gggcatcgca
     3901 gtactgatgt tgtctttgtt tattttaaaa gaaacgcgcc ctgcggcccc cacaacttcg
     3961 gacaaaccac gagaaaatag cgagtcactg cttaaccggt ttttcctcag ccgtgttgtt
     4021 atcaccaccc tcagcgtttc ggtgatcctc accttcgtca acacgtcgcc agtattgctg
     4081 atggaaatca tgggttttga gcgcggagaa tacgccacca ttatggcgtt gaccgctggc
     4141 gtcagcatga ccgtttcatt ttccacacca tttgcgctgg gaatttttaa gccacgtacg
     4201 ttgatgatca cctcgcaggt gttattcctg gcgacgggga tcacccttgc cgtttcacct
     4261 tcccatgcgg tttcgctgtt tggtatcacg ctgatttgcg ccggtttctc ggtaggtttt
     4321 ggcgtagcga tgagtcaggc gttagggccg ttttcattac gcgcgggcgt agccagctcg
     4381 accttaggta ttgcgcaagt ttgcggttcg tcactgtgga tttggctggc ggcggtggtt
     4441 ggtatcggcg catggaatat gctgatcggg attctgattg cctgtagcat agtgagcctg
     4501 ttgctgatta tgttcgtcgc gcctggacgc ccgattgccg ctcatgaaga aatccatcac
     4561 cacgcttgat ctcaatctgc tgctctgtct gcaactgctg atgcaggagc gcagcgtgac
     4621 caaagcggcg aagcggatga acgtgacccc ttcggcggtg agtaagtcgc tggcaaagtt
     4681 aagagcgtgg tttgacgacc cgctctttgt gaactcaccg ctggggctgt cgcccacacc
     4741 gctaatggtc agcatggagc aaaatctggc ggagtggatg caaatgagca acctgctgct
     4801 ggataaaccg caccaccaga caccgcgcgg cctgaagttt gaactggcag cagaatcacc
     4861 gctaatgatg atcatgctta atgcgctgtc gaaacggatc taccaacgtt acccgcaggc
     4921 gaccatcaaa ttacgtaact gggattacga ttccttagat gccattactc gtggtgaagt
     4981 ggatattggt ttttccggtc gcgaaagtca tccccgttcg cgggagctgt taagctcgct
     5041 accgttagcc attgattatg aagtgctgtt tagtgatgtg ccctgcgtct ggttacgcaa
     5101 agatcatccg gcactgcatg aagcatggaa tctggacacc tttttacgtt atccgcatat
     5161 cagcatttgc tgggaacaga gcgatacctg ggcgctggac aatgtgttac aggagctggg
     5221 gcgcgaacgc acgattgcta tgagcctgcc ggaattcgag cagtcactgt ttatggcagc
     5281 acagcccgac aatctgctac tggcgaccgc gccgcgctac tgccagtact acaatcaact
     5341 ccaccaactg ccgttggttg ctcttcctct cccgtttgat gaaagccagc aaaaaaagct
     5401 ggaagtccct tttacccttc tgtggcataa acggaacagc cgtaatccga agatcgtctg
     5461 gttacgggaa accattaaaa atctttacgc gtcgatggca taaccgaatc gtatgaaacg
     5521 cgacccaatt tcacaataaa atgtaaaaaa gttgtaataa gcttgtctga atcgaacttt
     5581 tagccgcttt agtctgtcca tcattccagt aaatgattac tcttgtattc ataatggacc
     5641 attaagcatg gagcgaaaaa tggcgactca ctttgcccga gggattttaa cggaaggaca
     5701 tctgatttct gttcgtctcc cctcccagtg tcatcaagaa gcccgaaaca ttccccctca
     5761 tcgtcaaagc cgttttctgg cgtccagagg tttactcgca gaactgatgt tcatgctgta
     5821 tggcattggc gaattgccgg aaatcgtcac cctgccgaaa ggaaaaccgg ttttcagcga
     5881 taaaaatttg ccttcgtttt ctatttccta tgccgggaat atggtcggcg tggcgttaac
     5941 aaccgaaggt gaatgtggcc tcgatatgga actacagcgt gcgacacgcg ggtttcatag
     6001 cccacacgcg cccgataacc acaccttttc cagcaatgaa tcgctatgga tcagtaaaca
     6061 aaacgatcct aacgaagcgc gggcgcagct catcacgctg cgccgaagcg tgctaaaact
     6121 aaccggtgat gttttgaatg acgatccgcg cgatctgcaa ctactgccca ttgccggacg
     6181 cctgaaatgt gctcatgtaa atcatgtaga agcgttatgc gacgcggagg acgtgctggt
     6241 gtggtccgtg gcggtcacgc ccacgattga aaagctcagt gtctgggagt tagatggcaa
     6301 acatggctgg aaaagcctgc ccgatattca cagccgcgcc aacaatccta ccagccggat
     6361 gatgcgtttt gcccagctct ctaccgtgaa ggctttttcg ccaaattgat agacaaccac
     6421 aggagtcatc atgtctgaaa aattgcaggt ggttacgtta ctggggagcc tgcgcaaagg
     6481 ctcatttaat ggcatggttg cacgtaccct gccgaaaatt gctccggcga gcatggaaat
     6541 caatgcgtta ccttccattg ccgacattcc cctgtatgac gctgacgtac agcaggaaga
     6601 cggttttcca gcaacggttg aagctctggc ggaacagatc cgtcaggctg acggtgtggt
     6661 gatcgtcacg ccggaatata actactcggt accgggtggg ctgaaaaatg ccatcgactg
     6721 gctttcccgc ctgccggatc aaccgctggc aggtaaaccg gtattgattc agaccagctc
     6781 aatgggcgtg attggcggcg cgcgctgtca gtatcacctg cgccagattc tggttttcct
     6841 cgatgcaatg gtgatgaaca agccggaatt tatgggcggc gtgattcaga acaaagttga
     6901 tccgcaaacc ggggaagtga ttgatcaggg tacgctggac cacctgaccg ggcaattgac
     6961 cgcatttggt gagtttattc agcgagttaa gatctaaata aaaaacccgc cagcaatcat
     7021 gcatggcggg tttttaacgc gctatcgatt ttagtgagcg tcgataaaca caatcttcag
     7081 gataaacagc agcgcaacga tgattacgca cgggctaatg tcacgcagac gtccggtacc
     7141 aatcttcatc acgcagtagg agataaagcc cagcgcaata ccttcggtaa tcgaaaagct
     7201 gaacggcatc atcacagcgg taataaacgc cggaacagat tcagtaagat cctgccagtt
     7261 cacgcgagca agacttgagg tcatcagtac gcccacatag atcagcgcgc cagctgcggc
     7321 atatcccggc accatccccg ccagcggcga caggaagata accagcagga acagcagacc
     7381 gacaaccact gccgtcaggc cggtacgccc cccaacggaa acgccggaag aagactcgat
     7441 atacgccgta acagaagaag tcccgataaa cgaaccggtc acggaagaga tgctgtcgac
     7501 atacagtgcc tgcttcatgc gcgggaattt acctttttca tccgccagtc ctgctttatc
     7561 ggtcacgcca atcagtgtac cggaggagtc aaacaggttg accagcatga aagagaaaat
     7621 cacccctgcc agcccgaggt taaacgaccc ggctaaatct acatgaccca cgacactcat
     7681 tacgctcggc ggcgcagaaa cgatgccatt gtagtggaca tcacccagca tccagcccag
     7741 cagcgtcgtc accacgatgg aaaccagcac cgccgcgtga atgttgcgtg aggccagaat
     7801 agcaatgatg aagaagccga ggatccccag cagtacgctg tgagaagtca gattaccgat
     7861 gctcaccagc gtttccgggt tagcgacaat cacacctgca ttcttcagtc ccatcatgcc
     7921 aataaacagg ccgataccgc tggtaatgcc cacacgcaga ctcaccggaa tgttggctat
     7981 catccagtag cgaacgcgga aaatcgtcag taacagcaga ccaatcgcgc cccagaagat
     8041 tgcgcccatc ccgacctgcc acggcaagcc catcgcctgc acaaccacaa aagcgaagaa
     8101 cgcattcagg cccatagcgg gtgccagtgc aactggcagg ttagcaaaca gtcccatcat
     8161 aatactgccg aatgcagcaa tcagacaggt agtaacgaag acggcgctgg tatccatgcc
     8221 agcaacgcca agaatttgcg ggttaacaaa aacgatgtaa accatcgtca ggaaggtggt
     8281 aaaaccggcg atcacttcgg tccgtgccgt cgtgccatgt tcgcgcagtt taaacacgcg
     8341 ttccagcatc ccctggccag aagcctgggt tgtgtgttgt tgactcatta tctatttccg
     8401 aacaaggagg gaaaatccgt cgctatcgta taccaaaatg agacaatatg gacggctgaa
     8461 gataactttt tttcattgta tttgcttata cggcaacgat tgcgtgatgc gaattggcat
     8521 gacgaaaacg ttaaactaac gataaaggaa agacatgtcc cagatagaag cggtattttt
     8581 cgactgcgac ggtacgctgg tcgatagcga ggtcatttgc tctcgcgcct atgtcacgat
     8641 gttcagggaa tttggcattc acgttgacct ggaagagatt tttacccgtt ttaaaggcgt
     8701 aaaactctac gaaatcatcg atatcatcag taaagaacag ggcgtgacga tggtccaggc
     8761 cgatgccgag cacgtttacc gcgcagaagt agcacgtctg tttgataccg aacttgcgga
     8821 catcgcagga gcgaacgaat tgctggcgag cattaacgtg ccgatgtgcg tcgtctctaa
     8881 cggtccggtc agcaaaatgc agcattcgct tggtaagctc aacatgctgc actacttccc
     8941 ggaaaaactg ttcagcggct atgatatcca gcgttggaag cccgatccgg cattaatgtt
     9001 ccatgcggca aaagcgatga atgtgaacgt tgaaaactgc attctggtcg atgactccag
     9061 cgcaggtgcg caatcaggga tagatgccgg aatggaagtg ttttacttct gcgctgaccc
     9121 gcacaataaa cagatcgttc acccgaaagt caccaccttt acccatcttt cgcaattacc
     9181 ggaactgtgg aaggcgcgtg gttgggatat tacggcatag ttattcacac tcccttcact
     9241 tactccactt aaattggcac gcaaaggtaa gtaaagggag tttgatatgt ctgtttcacg
     9301 tcgggtaatt catcacggac tgtattttgc ggttttaggg ccgttaattg gtgttctgtt
     9361 tcttgtcctc tacatattct tcgcaaaaga accgctgatt cttttggtaa taatacaggt
     9421 acttccattg tttctcttaa tgtcgataac tacgggagct attcctgcaa tgttaactgg
     9481 cgtaatggtc gcctgtctgc cggaaaagat cgggtcacag aaacgttatc gttgtctggt
     9541 tggcggcata ggtggagtcg ttatcaccga gatctattgc gctgttatcg tacatattaa
     9601 ggacatggct tcctcagcgt tgtttgaaaa cattctttct ggtgaaaatc tcgttgtccg
     9661 catcatccca gcattgctgg caggcgtagt gatgagcaga atcattacac atctacccgg
     9721 attggatatt tcatgtcctg aaacagactc tttaagctaa gcggaatgct ttatctattt
     9781 gtcattaaat gttggagaac aggccggagc gtaattcaca catccggcct tatttttaag
     9841 cttaaccgag cgctaattct gcggcagcgg ctttatcagc gatgactatc agcgaagggt
     9901 gcaactgcaa tacggaagct ggaacatctt cggtaaccgg accttgcaga acatttttca
     9961 gtgcctgcgc tttacctgcg ccactgacaa ttatcagcaa gtttttcgcc gccatgatgc
    10021 ttttcggccc catcgtcacg tagctgtcag gcactaatga aaagtcgccg cccaactcgc
    10081 catgcgccac aatatcaacc atctctccct ggatcgggaa ctccacggtt tgctcatgga
    10141 aatgggtcgt atttggcaga ttgccacaaa aatgaccatc tgcacctaat cccaacacca
    10201 ccaaatccag gccgccttca cgcgccagtt tctgatcatg ctcgcggtag ttatcaatag
    10261 tgagcttctg gatactctct tctttgatcc ccgcaggggt gaaaaacaga ttacgcagat
    10321 tggtaatcgt tacgccttct ccctctttgc cgcgaaatgg aatttcatcg aaattgtaga
    10381 aatagcagtt atcgtaccag ggcttacctt ttaccagagt ggtgaggtat tcgtacatgc
    10441 ctttgggcgt gctcccggcg gtaattgcca ggttaacacg gcgcatcttc gacatataac
    10501 caagcagatg atgtgccgcg acacggctca tttcctggta atcttcggta atgatcaatt
    10561 tcatcttaat gccttatttt atttaaataa tttctgtaca aaggctgcat aggctggtcg
    10621 ccagacatcc atttcatggt tcagaccggg atattcctgg taatcaaact taattttttt
    10681 ctgctcaagc tcagttttca gcccggcgat atccttgccg gttacgacat ctttatcgcc
    10741 cacgaccaca gtaaaattac gtagttgctg gttaatagct gccggctcgt tcagccgggc
    10801 agcgacccct tcatctggta cggtcgtcgt ggtaacacca ctgaatgtgg ccagccagcc
    10861 aaagctttcc agatgattca ttcccgatac cagtgcctga tacccacctg gtgaaagccc
    10921 tgccagtgcg cgaccatcgg catctttacg gacattaaaa cgcttgctaa tcagcgggat
    10981 aatatcgttc atcaattcgc gatctgccgc tttagcattt aacggataaa agactttacg
    11041 tctctcctga ggaacgaaat cttcgggaat aatgcccttc gcatcggttt cggtatccgg
    11101 gatcaccacc agcatcggtt taattttccc ttcagccagc aggttatcca tgatttgcgg
    11161 gatacgcccc tgatcaatag cggaacgtcc ggtatcacca aagccgtgat agaaatagag
    11221 cactggcaaa ggctcgccta tgccggtgta tcctggcggg gtccagacat acatctgacg
    11281 ttcggattgc aatgcgttgg agtggtaagt tatggcgatc aaatcaccat gcacaacaga
    11341 acgtgtatcc agataactgc ccggtaccag aatcatactg gagttaacct ggcgctgagg
    11401 cttagtcatt gctgtacctg tatcaatgct gcgtacacca tcaacattga aaaaatactc
    11461 gtacaggttg cctttcagag caggcgtgcg ccacgaccag actcccgctt cgtctttggt
    11521 catcgggtga atattgtccg gaaccggaac acccaccact acagagacat tttttgcccc
    11581 aggggcaaag tagcgaaagg tcacgctgtt atcggtattg acctgagtca catactgttt
    11641 caccggaata gtgggtgccg ggctggctgg catatccgct gctatggccc attgagcaga
    11701 aataccgctg gcaatagcca gcgttaaagc agcaattttt atattcatcg taatattcct
    11761 tttattaagt tccattacca ccagatttca gcctgggcac cgacagcaaa ctggtcgtct
    11821 ttattgtctt caaaggtgaa gccatccagt tcgttttcca gagctttgat atatgtagcg
    11881 tagaaacgaa tttccgggcg cgaggtcaac atactggtat tgactttaaa ggtatggaag
    11941 agcgtggttt tataaccaga ctcattatat ttattgctat tcgcatcttt gttttgctgg
    12001 gtgaaatagc ccagttcaac acctgtctgg ttatattggt cccaaatata ggccgggcga
    12061 acaaccgcac gaatagattc gaaatcagaa tgtgcgcctg tttcgtagct ataaatatcg
    12121 ttaccgaagg agtaaacaat cgcgttagcc acaatgaaat gatcgccgat ataagcttcg
    12181 ccctgcgaag tcagacgaac tgcagttccg cctgtgtgat caccataata acgaccatta
    12241 aaggtagtaa atggacttgc accagcataa cgaccaaagt tactggcgat agagttattc
    12301 gcaaccagga aggagaattc gttgaagcca cccttatcaa atttctgcgt taaagatgta
    12361 ccaaacatcc aggtatcttt ccagtcataa tacccgttat tatcctgatt atctttttcc
    12421 gatgcgcttt catttgctga gacataacaa ccgctcacca ttaaggtggc tttatcccat
    12481 aacgggatat ctttatagcg taaatcaatg gtattggtat taatctgctg tttgttttgc
    12541 aggctgcgat cgtaatcatc gacatcttca cgaaccagcg cgatatcaat tttacccgga
    12601 ccgactttcc agttttccag acctacacct gcggcagcat cggtacgctg cgttttccag
    12661 tcaagcatct ggatttcaat tttcggcgca ccgtgtttac ccacccagaa atcagcctct
    12721 ggcgcaaagg gcaggaaacc tttggtggta acgtacatat cagagaactg cataaagttc
    12781 tcaccaccgg cgttatcgcc aaaccagccg gtagaatact gctgaccaac gttaccatcc
    12841 atcatcacaa cggcatcaac ccgtttgccg ttttcgttgt agactcgttg ttttaactgc
    12901 aaatcaaacc aaccggagta ttcgttacca aagcggccaa gagaaccaat cgcccatgat
    12961 ttaggtgaac catgagaggc tgtaccccaa ccagaacgat aatagccgtt gtaactaaag
    13021 ccaatttcat ctttcacaaa tttactgaaa tctttcagtg tcatggaaga ataaatcgag
    13081 cccgtctcac tggcattttt ctcttcattt ttgaccagta cggcatcagg ttttgcggca
    13141 ctactggtcg ggaacggatt ggtggcatat ccttgatcat tcgtacttac gctacgattc
    13201 accgtcgctg gcgtatactt tttcttctct tcatctttat attttttcag ttcgctttgc
    13261 gtttctctta acgccttttc taatagctca aggcgttgtt ccactgttaa tgattcagcc
    13321 aatgattgtg cggaaaaggc taacggtgcc attaataaga tggcagaggt aataagattt
    13381 cgtctaaaca taatatccct ttatggtgca aagaaagaat taacgcatcg catccagact
    13441 gttctggatg cgacgataat taaggtactt tattggttat ttttttaatg atagcccccg
    13501 agttttgatc acttctgcat accatccgaa gcttttctta cgcgttcttg tgaggcttcc
    13561 ttcaccatta tcatcacgat ccacataaat aaagccgtag cgcttagaca tttgtgaatg
    13621 agatgcactg actaaatcaa ttggccccca actggtgtac cccataatat ccacaccatc
    13681 ggcaattgct tcatttacct gtaccaggtg atcgtttaaa taggcaattc gataatcgtc
    13741 ctgtatcgaa ccatccactt caacgctgtc ttttgcgcct aatccgttct cgacaataaa
    13801 taacggtttt tgataacgat cccaaagcgt atttaacaga atccgtaatc caaccggatc
    13861 aatttgcccc ccccactctg aacttttcag atgcggattt gggatcatat tcagtatgtt
    13921 gccctgcgca tttttattaa tgctttcgtc gtgggaaaca caaccggtca tgtaataact
    13981 aaaagagatg aaatctacgg tatgttttaa atcttctgcg tcactttcag tcatctcaat
    14041 ggtgatattg tggtcgcgga agaaacgctg catatagccg ggatactgac cacgcgcctg
    14101 aacatcacca aagaacatcc agcgccgatt ctcctccatg gcctgcaaca tatcctgtgg
    14161 ctggcaggtg agggggtaaa ccagcccacc gagaagcata ttgccgattt tcgcttcggg
    14221 gatcaggcta tgacaggctt taactgcccg cgcactggca accagttgat gatggatcgc
    14281 ctgataaact tccgcctcgc cactctcttc tgccagcccc acgcccgtga atggcgcgtg
    14341 taatgacatg ttgatttcat taaacgtcag ccataacgct actttatgtt ggtagcgggt
    14401 aaagaccgtg cgtgcgtaat gctcgaagtg atcgatgacc gcccgattag cccaaccgcc
    14461 gtagtttttc accagcccat atggcatttc gtaatgggat aacgttacca gcggcttgat
    14521 ccccacctgc gccatttcat caaacagccg atcgtaaaac gctaaccccg cttcattcgg
    14581 ttcggcttcg tcgccctgag gaaaaattcg cgcccaggca atggaaatac gcagacaggt
    14641 gaagcccatc tcggcaaata acgcgatatc ttccgggtaa cggtgataaa aatcgatggc
    14701 gacatctttg atattctctt tccccaggat gcgcggttcc atttttccca ttacgccatg
    14761 aggctgtaaa tctgaggtcg tgatcccttt gccatcttcc tgccaggcgc cttccacctg
    14821 attggcagct gttgcaccac cccaaagaaa tgtttctgga aatgctttca taattaactc
    14881 cttttatcgt tagcgaatga tggataacag cggctcacca gcgtttatct gcgccgtgcc
    14941 gtggggtaat acgtccgtaa aatcatcgct attactgatt aataccggcg tcgtcagatc
    15001 aaatccggcc tcgcgaatag cagggatatc aaaagaaatc agccgatcgc ctgtattgac
    15061 cttgtcaccc acgttgacgt gagcggaaaa gaatttgccg tccagtttta cggtgtcgat
    15121 accgacatga atcaggatct ccacgccatc atctgactca atgccgatgg catgtaatgt
    15181 ggcgaacaac gaagcaattc gacccgccac cggagaacgc acttcaccaa cagagggcag
    15241 aatggcaata cctttaccca acaggccgct ggcaaacgtg gtatcagcga cgtgaatgag
    15301 cggcacaatc tctcccgtca tcggtgaaca gataccgccc tgctcaggtg taataacctc
    15361 tggtgttttc tcttgctggg caacttgcgc tgactgactc ttagcggtga tgaaatgaaa
    15421 catcaccgta ccgacaaatg cgcaaccgat ggcaatgaca ccgccaataa cgctggccca
    15481 tacggtgaaa tcaatccccg ttgacgggat ggtttgcatg aaggtgaaaa tacctggcaa
    15541 accaaaggag taaactttcg tttgcgcgta gccaatgatg gtggccccca atgccccact
    15601 tatgcaggcg ataacgaagg ggtacttacg cggcagattg acgccatata ccgctggttc
    15661 ggtgatacca aacaaacctg tcaacgccgc tgatcccgcc accacttttt tctgtgcatc
    15721 gcgttcgcag aggaagacgc cgagcgccgc cccgacttgc gccataatgg cgggcattaa
    15781 cagcgggatc atggtgtcgt agcccagcac ggtgaagtta ttgataatta aaggtatcaa
    15841 accccaatgc agaccgaaca tcacaaagat ttgccagaag ccgcccatta ccgcgcctgc
    15901 aaatgcagga accgcctgat aaagccagag ataaccggca gcaatcagtt cacttatcca
    15961 ggttgatagc ggccccacca gcagaaatgt gatgggcgtg atgaccatca gacatagcaa
    16021 gggtgtgaag aaatttttga ttgccgaagg taaccacgca ttaagtcggc gttccagaat
    16081 gctgcacaac caggcagaaa aaataatggg aataaccgat gacgagtaat tcaacaatgt
    16141 gaccggaata cccaggaaat ccagccccag cgcatccact ttttgcccgt tctcgaaggc
    16201 agtcagaatt aatggatgca ctaacgctcc gccaatcacc atggcagtaa atggattgcc
    16261 gccgaagcgt ttccctgcgg tgtagcccag gattatcggg aagaaccaaa acaaggcatc
    16321 actggcgcta aataaaatta aataagtacc actttgttcg gtcgcccact gaaaagtgag
    16381 tgccagagcc aacatacctt tcaggatccc ggttgccgcc atcaaaccga tcagaggcgt
    16441 aaaaatacct gaaataacat aaacaaagcg gtttaacaga ttatctttat catcattttc
    16501 cggtgcctgt tgcgcttttt cgccaaggcc tgccacgctg ttaaccgcca ggaagacatc
    16561 ggccacatgg ttacctatga caacctgaaa ctggccaccg ctttccacca ccataataat
    16621 accgggggtc tttttcagta cctcagcttg cgctttgctt tcatccttta acttaaaacg
    16681 taatcgcgtt gcacaatgca tcagactcac aatgttatct gcgcccccga ctcctgcgac
    16741 tattgttctg gctaactccg tcataacttg ccctctaccg ctttgcggca aaactccaaa
    16801 aaaaaacctg aaaaaaacgg cctgacgtga atcaagcaat ttttttcagg ttttgcccgc
    16861 ttagtgcggt aacaatcctt tattcagtaa taatatttca gtgttctttg cgcacgcgct
    16921 ctatatttat ggctaaaaac ataatctctg caggtgagat tttacgttga tactgcaaac
    16981 caataaaaat ggcgatccgc tccgcacatt gccatgcttg cgggtaattt tgctttactg
    17041 cttgttgtaa tgattcatca ctatcgttaa tcgatgcatg ttcaagaata cgccaggata
    17101 aaaatttcag atgcgtaacc agtcgctgat aactcaagct ttcttcctgg taattaaggc
    17161 tgaactgaaa ttttattaat tgcagcattt ctcgcattaa ttgcgtgaca cctgcaacat
    17221 cctccatatt tccgctcatt tgggcactga ccagatgcat ggcaataaag cccacttcat
    17281 ctttcggtaa ctgcacgccc aaccgtttat caataatggt taacgcttct tcccctagct
    17341 ggaactcttt cgggtaaagt cgctggatat cccacagcag cgggttgggc agtagcacgt
    17401 tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt tagcgagata taaatactgt
    17461 cctgcaactt tcccagacgc tcctgcgcca gagagataat acgatcacag gttgccatca
    17521 cctcaagagg catatgactt aagagttcgc ttaatcgccc gttcagttca tgactgctca
    17581 aggcatactc tttttctatt ccacttgagt taattctttc gcctgggcgt ttttgaaagc
    17641 caattccgcg ccccatgacg actttttccc gctgttgatc atcaataacc accacaacat
    17701 tattgttgag aattttggtg atttgcatgt tcatagcaag gacctttttt ataaacaaaa
    17761 aaacccgact tcaccagtat tctctggtta tgtcaggttt tgcctgcgaa tgcagtaaca
    17821 atccagtcat taatcaatgg tttttataac gaacatccag gttcgaaaat taatttagtt
    17881 gcgtgcagag aaaatagcaa tgctctattt ataaaagtat gaccatgc
//
LOCUS       DAPHCV010000100        17483 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.100, whole genome shotgun sequence.
ACCESSION   DAPHCV010000100 DAPHCV010000000
VERSION     DAPHCV010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.100"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1330
                     /locus_tag="RH033_003538"
     CDS             <1..1330
                     /locus_tag="RH033_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HDV2992669.1"
                     /translation="DDDETLYDLLGTKEYRKELDKQFKNAKSNFKIAIVVDMWLTGFD
                     VPELDTIYIDKPLQKHNLIQTISRVNRKLEGKSKGLVVDYIGIKSQMNQALAMYSRID
                     ATNFEDIQQSVIEVKNHLDLLGQVFYEFDSRDYFSGEPQAQLSCLNRAAEFVLRTQKV
                     ERRFMGLVKRMKAAYDVCCGSEALSQTERDYIHYYLAVRSIVFKLTKGDAPDVTQMNA
                     RVREMIAEALKADGVEEIYFLGDKKAESIDIFDEDYLARINKIKLPATKIQLLQKLLE
                     KAISDFRKVNQLQGINFTRRFQAIIDRYNERREDDVLNGEEFDTFSQEMTDIIYDIKT
                     EMGTWADLGIDIEEKAFFDILAHMRDKYQFTYDDEKMLSLAKEMKSVVDNTSKYPDWS
                     KRDDIKAKLKVELILLLHKHKFPPVANDDVYMGVLAQAENFKEHHMSALN"
     gene            1545..1883
                     /gene="symE"
                     /locus_tag="RH033_003539"
     CDS             1545..1883
                     /gene="symE"
                     /locus_tag="RH033_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HDV2992670.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLETAGFATGTAVDVKVIEGCIVLTAQPPAAEESELMQSLRQVCKLSARKQRQVQDF
                     IGVISSKTPR"
     gene            complement(1925..2089)
                     /locus_tag="RH033_003540"
     CDS             complement(1925..2089)
                     /locus_tag="RH033_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HDV2992671.1"
                     /translation="MEFHENKAKTPFIRLVQLWQAVRRWRRQMQARRVLQQMSDERLR
                     DIGLRREDVE"
     gene            2266..3678
                     /locus_tag="RH033_003541"
     CDS             2266..3678
                     /locus_tag="RH033_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDV2992672.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLVAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQVARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQIYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(3920..4863)
                     /locus_tag="RH033_003542"
                     /pseudo
     CDS             complement(3920..4863)
                     /locus_tag="RH033_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            5330..6562
                     /gene="mdtM"
                     /locus_tag="RH033_003543"
     CDS             5330..6562
                     /gene="mdtM"
                     /locus_tag="RH033_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HDV2992673.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADISLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGIIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            6603..7883
                     /locus_tag="RH033_003544"
     CDS             6603..7883
                     /locus_tag="RH033_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HDV2992674.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKVDINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDAQDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            7999..9150
                     /locus_tag="RH033_003545"
     CDS             7999..9150
                     /locus_tag="RH033_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
                     /protein_id="HDV2992675.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVEDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETSDVYDALADKYLAIGCSCVSPNDQRLKMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            9160..9927
                     /locus_tag="RH033_003546"
     CDS             9160..9927
                     /locus_tag="RH033_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992676.1"
                     /translation="MAYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVRPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQRF
                     THMLESHLGMPVQTHPDAQFAGAIGAAVIGQRQRKRA"
     gene            9924..10181
                     /locus_tag="RH033_003547"
     CDS             9924..10181
                     /locus_tag="RH033_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HDV2992677.1"
                     /translation="MKEYLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGHTESVYCQQDGGWRCIAHYGISPR"
     gene            10246..11106
                     /locus_tag="RH033_003548"
     CDS             10246..11106
                     /locus_tag="RH033_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HDV2992678.1"
                     /translation="MTKSISLSKRIFVIVILFVIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDLIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQESPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDKALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLAGEVIGEMSLTKGSRGLSHNIKQAEGVAMDASGNIYIVSEPNR
                     FYRFTPQSSH"
     gene            11174..12352
                     /locus_tag="RH033_003549"
     CDS             11174..12352
                     /locus_tag="RH033_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2992679.1"
                     /translation="MPSPSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GSFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLASALASGLLILAMAWL
                     LPFILVLLIRVLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFDLSSQTLWQGAGALSGMMLIALTLLMPSKKHAITPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGSTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(12365..12919)
                     /gene="kptA"
                     /locus_tag="RH033_003550"
     CDS             complement(12365..12919)
                     /gene="kptA"
                     /locus_tag="RH033_003550"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HDV2992680.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKALEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            13169..13852
                     /locus_tag="RH033_003551"
     CDS             13169..13852
                     /locus_tag="RH033_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HDV2992681.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGTGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            13849..14310
                     /locus_tag="RH033_003552"
     CDS             13849..14310
                     /locus_tag="RH033_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HDV2992682.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            14323..15495
                     /gene="iadA"
                     /locus_tag="RH033_003553"
     CDS             14323..15495
                     /gene="iadA"
                     /locus_tag="RH033_003553"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDV2992683.1"
                     /translation="MIDYSAAGFTLLQGAHLYAPEDRGICDVLIANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQMLVKDYSFSLSDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            15560..16471
                     /gene="hypT"
                     /locus_tag="RH033_003554"
                     /gene_synonym="qseD"
     CDS             15560..16471
                     /gene="hypT"
                     /locus_tag="RH033_003554"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HDV2992684.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIEPG"
     gene            16478..16849
                     /locus_tag="RH033_003555"
                     /pseudo
     CDS             16478..16849
                     /locus_tag="RH033_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519422.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(16897..17280)
                     /gene="iraD"
                     /locus_tag="RH033_003556"
     CDS             complement(16897..17280)
                     /gene="iraD"
                     /locus_tag="RH033_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HDV2992685.1"
                     /translation="MMRQSVQTVLPESTGHNTLSLRDSVCRDLFQLFSSPHSPLPILL
                     VSGMPEWQGHNQSDKLLQSWYCRQLRSALLFHEPRIAALQVNLKAVYCHELVISLEMM
                     LYHDDEPLTFDLVWQKGSWHRTMPQ"
BASE COUNT         4096 a         4391 c         4449 g         4547 t
ORIGIN      
        1 agatgacgac gagacgcttt atgatttact gggcacaaaa gaatatcgca aagagctgga
       61 taagcagttc aaaaacgcta aatcgaattt caaaattgcc attgttgttg atatgtggct
      121 gaccggtttt gatgttcctg aactggacac catctatatt gataagccct tacaaaaaca
      181 taaccttatc cagactattt ctcgcgttaa ccgtaaactg gaaggcaaaa gcaaagggtt
      241 agtagtggac tacatcggca ttaagagtca gatgaaccag gcactggcaa tgtattcccg
      301 cattgatgcc accaactttg aagatattca gcaatcggta atagaagtta aaaaccatct
      361 cgatttgttg gggcaagtct tttacgaatt tgacagtcgg gattatttta gtggtgagcc
      421 acaagcgcaa ttatcctgcc tcaaccgcgc ggcggaattt gttctgcgta cccagaaagt
      481 tgaacgtcgg tttatgggac tggttaaacg catgaaagcc gcctacgacg tctgctgcgg
      541 cagcgaagca ttatcacaga cagaacgtga ctatattcac tattatcttg ctgttcgttc
      601 aattgttttc aaactgacga aaggtgacgc accggatgtc acccagatga atgctcgcgt
      661 tcgtgaaatg attgcagaag cgttaaaagc tgatggcgta gaagaaattt attttcttgg
      721 cgacaaaaaa gcggaatcca tcgatatttt tgacgaagat tatctggcgc gaattaacaa
      781 gatcaaactt ccggcaacga agatccagtt attacaaaaa ttactggaaa aagcgatcag
      841 cgacttcagg aaagtgaacc agttgcaagg gattaacttc acccgccgct tccaggcgat
      901 tatagatcgt tataatgagc ggcgagaaga tgatgtactc aacggtgaag aattcgatac
      961 attcagtcag gaaatgaccg atattatcta tgatattaaa accgaaatgg gcacatgggc
     1021 cgatttaggt attgatattg aagaaaaagc gtttttcgac attcttgctc atatgcgcga
     1081 taaatatcag ttcacctatg acgatgaaaa aatgctgtca ctggcaaaag agatgaaaag
     1141 cgtggttgac aacacatcga aatatcctga ctggagtaaa cgcgatgata ttaaagcgaa
     1201 actgaaagtt gaacttattc tgcttctaca caagcataag ttcccgccag tagcgaatga
     1261 tgatgtttat atgggtgtgc tggcacaggc agagaatttt aaagaacatc atatgagtgc
     1321 tttaaattaa ttatgaaggc cggatacact ttgttatccg gccattctga attaacgtta
     1381 atttctcttc cagatctttc cagtaacgct tttattattt tctattttta gctggacttt
     1441 ccccatattt actgatgata tgtccagata tttagcgcgg tgcggatgtg cgccaacaca
     1501 cccgcaccgc taatcacaat ccctatttca aaggaatagt aatcatgact gacacgcatt
     1561 ctattgcaca accgttcgaa gcagaagtct ctccggcaaa taaccgtcaa ttaaccgtga
     1621 gttatgcgag tcgctacccg gattacagcc gtattcccgc catcaccctg aaaggtcagt
     1681 ggctggaaac cgccggtttt gccaccggca cggcggtaga tgtcaaagtg atagaaggct
     1741 gtattgtcct caccgcccaa ccacccgccg ccgaagagag cgaactgatg cagtcactgc
     1801 gccaggtgtg caagctgtcg gcgcgtaaac aaaggcaggt gcaggatttt attggggtga
     1861 ttagtagcaa aacgccacgc taagtttctc tccccttccc catacgggaa gggaaattgc
     1921 cccctcactc cacatcctcc ctgcgtaacc caatatccct caatcgctca tcactcatct
     1981 gctgtaacac ccgtcgggcc tgcatttgcc gccgccaacg cctcaccgcc tgccagagtt
     2041 gcacaagtct gataaacggc gttttggctt tgttttcgtg aaattccatc tgctgcctcc
     2101 tcatctttca gaggcttcat catccgcctt ctggcagttc acaatacaga tgcaaatatt
     2161 agttttattt aacatacaga caggttaaaa caacatctgc atccggtttt ttagcctcat
     2221 ctgtaccggt ttttctctct gtatggtcca aataaggaat acagcatgac gcgttatcaa
     2281 catctggcga ctctgcttgc cgagcggatt gagcaagggc tgtatcgtca cggggagaaa
     2341 ttgccgtcgg tgcgcagctt aagtcaggag cacggcgtca gtattagcac cgtgcagcag
     2401 gcgtaccaga cgctggagac gatgaagctc atcactccgc agccgcgttc gggttatttt
     2461 gtcgcacaac gtaaagccca gccgccagta ccgccgatga cgcgtccggt gcagcgcccg
     2521 gtagagatta cccagtggga ccaggtactg gatatgctgg tagcgcacag cgacagttcc
     2581 attgttccgt taagcaaaag cacgccggat gtcgaaacgc ccagcctgaa accgctgtgg
     2641 cgggagctta gccgggtagt gcagcataat ctgcaaaccg ttctcggtta tgacttgtta
     2701 gccggtcagc gagtgttgcg agagcaggtt gcccgcctga tgctcgacag cggctcagtg
     2761 gtcaccgccg atgacatcat tatcaccagc ggctgccata actcgatgtc gctggcgtta
     2821 atggcggtat gtaaaccggg cgatattgtc gcagtcgaat ccccctgtta ttacggttca
     2881 atgcagatgc tgcgcggtat gggcgtgaaa gtgattgaaa tcccaaccga tccagaaact
     2941 ggcatcagcg ttgaagcgct ggaactggcg ctggaacagt ggccgattaa aggcatcatt
     3001 ctggtgccaa actgtaataa tccgctggga tttattatgc cggacgcgcg caaacgggcc
     3061 gttctctctc tcgctcagcg tcatgatatt gtgatttttg aagatgatgt ctacggcgaa
     3121 ctggcgacgg agtatccgcg cccgcggacc attcattcct gggatatcga cgggcgagtg
     3181 ctgctgtgca gctcgttcag taaaagtatt gcaccaggcc tgcgcgtggg ttgggtcgca
     3241 ccggggcgtt atcacgataa actgatgcat atgaaatacg ccatcagcag ctttaatgtg
     3301 ccgtccacgc aaatggcggc ggcaacgttt gtgctggaag gtcactatca tcgccatatc
     3361 cggcggatgc ggcagatcta tcagcgcaat ttggcgcttt atacctgctg gatacgggaa
     3421 tattttccct gcgaaatctg tattacgcgc ccgaaaggcg gatttttact gtggatagaa
     3481 ttgcctgaac aggtcgatat ggtctgcgtc gcgcggcagc tgtgccgcat gaaaatccag
     3541 gtggcagcag gctcgatttt ctcggcttcc ggcaaatacc gtaattgtct gcgcatcaac
     3601 tgcgctttgc cgctcagcga aacctatcgc gaagcactaa agcaaattgg cgaagccgtg
     3661 tatcgggcaa tggaatgaat tcgaaatagt ggcgagcccc ctgatttcct ctcacgaatc
     3721 gcgtgtgact cgttttatct ttgcagggtc atcgccacgt tccagcatgg cttaggcaaa
     3781 gtgtgctttg ttcataccgg atgcggcgtg aacgccttat ccggcttaca aatcgcgcaa
     3841 attcaataga ttgcagaggt aatgtaggtc tgataagcgt agcacatcag gcagctttgc
     3901 gtttgtcatc agtctctgat tagtactcga tccatggatc gcgcttgctt tcaggcaagg
     3961 gttgctctaa tgcctgcatt tgctctgccg aaagtccagt atatcgttgt atccattcag
     4021 gatccgcacc gttctgcaaa agcaatctaa ggaggcgttg ttccccttct tctttcccct
     4081 tttccatccc taacagccat ccatcattat gaattcgctc tgcaatcgtc attattcgct
     4141 ccctttgttg tggcattcga cgggtaagtt cactgataaa ctcattaaaa cgcgcttcat
     4201 cgccagtcag taaaatgtaa tttaacagcg cagttatctg gctgtcatta gcacactctg
     4261 taaccagtaa tattaccagt tgatcgataa gccccatcag atcgcgctgg cgaatatgct
     4321 tttggatcaa ctccaacagg gcgactctgc ggtgctgcac aatctcgtcg tctggcacga
     4381 cagtgacatc caccagcggg aacgctgcgg tataaagctt ccgtgcggtg gtcgggtcag
     4441 caaattcgtc cagccagcac agggacaggg ataaggacta cggctaccgt gataaaacag
     4501 catcgggatg accagcggta gcggtcggcg tttatcatgc tcgatatggc gctgcatcac
     4561 cgccatggaa tatcgcatca ggcgaaaggc catatggata tcctcgcggc tctgatgttc
     4621 aatcactacg taaatataac catcaccttc acgggtcttt accgaccaca gaatatcgga
     4681 gtgtagcgcc cgcaattttt catcgacgaa gctggcggat tccagtttta agctgtcgag
     4741 atcgcacagt tcacgtaaat ctttgggtaa gtggatctcc ataaaatccc gcgcggtgtc
     4801 agggtgcgtg agaaaggatt taaataatgc gtcatgcggc gtgctggttg tgaagtttgt
     4861 catggcgatc cgtcacctgt agataagatg gcccgactgt agcgactaac aaaaccattc
     4921 tcacccggca atttctcgtt ccccgaggcg ctttccaggc tatttttgtt cctctgcaaa
     4981 cgatacattt ttccggaaac aggctcgcgt tttacatttc ctgtacccgg cagatattcc
     5041 cctttccacc gcactctttt gattaaccgc aaattttaat tacacttaag gtgtatattt
     5101 tctatgcaac ccatcaattc aagaggtgta atgtgctgat gactatttgt aatcgttata
     5161 cattctgacc tggagtcaga aggtatttct ctgtctgtgt gttcacaggc agtgtggttg
     5221 attacatgaa attcagtaca tttgtccttc tcgcctgcct ttcgtcatta ccgacggtat
     5281 tgaatttcgt tttccccatt ggggttctcc ggacaaggag ttgtttgtta tgccacgttt
     5341 ttttgcccgc catgccgcca cgctgttttt cccgatggcg ttgattttgt atgactttgc
     5401 tgcgtatctg tcgacggacc tgatccagcc ggggatcatt aatgtggtcc gtgattttaa
     5461 tgccgatatc agtctggccc ctgctgccgt cagtctctat cttgctggcg gtatggcgtt
     5521 acagtggctg ctggggccgc tttccgacag aattggccgc aggccggtgc tgattaccgg
     5581 ggcgttaatt tttacccttg cctgcgccgc gacaatgttc accacgtcaa tgacacaatt
     5641 tcttatcgcg cgtgcaattc agggcaccag tatctgtttt attgccaccg ttggttatgt
     5701 cacggtgcag gaggcgtttg gacagacaaa agggatcaag ttgatggcga ttatcacctc
     5761 catcgtactg attgcgccga ttatcggccc gctttccggc gcagctctga tgcactttgt
     5821 gcactggaaa gtcctttttg ccatcattgc ggttatgggt tttatctcat ttgttggctt
     5881 actgctggcg atgccagaga cggtgaagcg cggcgcggtt ccatttagcg ccaaaagcgt
     5941 cttgcgcgat tttcgtaatg tcttttgcaa tcggctgttc ctctttggcg cagcaaccat
     6001 ctctttaagc tatatcccaa tgatgagttg ggtggctgtc tcgccggtga tcctgatcga
     6061 cgcgggcggc ttaacaactt cgcagttcgc ctggacacag gttccggtgt tcggcgcggt
     6121 gattgtcgcg aatgccatcg tggcgcgttt cgttaaagat ccgaccgaac cgcgctttat
     6181 ctggcgtgcc gtacccattc aactggtcgg cctcgcgctg ttgattatcg gcaatctgct
     6241 gtcaccgcac gtctggctgt ggtcggtgct gggcaccagt ctgtatgctt tcgggattgg
     6301 tttgatcttc ccgaccttgt tccgctttac gctgttttcc aataacttac cgaaaggaac
     6361 cgtctccgca tcgctgaata tggtgatcct gatggtgatg tctgtctcag tcgaaatcgg
     6421 ccgctggctg tggtttaacg gcggtcgctt gccgtttcat ctgttagccg tcgtggcggg
     6481 cattatcgtc gttttcaccc tggcaggatt gcttaatcgc gtgcgccaac atcaggctgc
     6541 cgagctggcg gaggagcggt gatttttgcg cgatcctgcc gtcaggctct attcttaacg
     6601 ttatgaataa actcattgaa ctcagacgcg ccaaaatgtt ggcgctctct ttactgctta
     6661 tcgccgctgc tacctttgtc gttacgctgt ttttgccgcc caatttttgg gtgagcggcg
     6721 tgaaggcgat tgctgaagcg gcgatggtcg gcgcgctggc ggactggttt gcggtggtgg
     6781 cgctgtttcg ccgcgtgccg attccgatca tttctcgtca tacggcgatt atcccgcgta
     6841 ataaagaccg gattggcgaa aatctcggtc agttcgtgca ggaaaaattt ctcgataccc
     6901 agtcgctggt ggcattgatt cgacgtcacg aaccggcgtt gttgattggc aactggttta
     6961 gtcagccgga gaacgcccgc cgcgttggtc agcatctgtt gcagatcatg agcggttttc
     7021 ttgaactgac cgatgatgcg cgtattcagc gcctgcttaa gcgcgcggtc caccgggcga
     7081 ttgataaggt tgatctttct ggcaccagtg cgttgatgct ggagagtatg accaaaaacg
     7141 atcgtcatca ggtgctgctc gatacgctga tcgcacagtt gatcgccctt ctccagcgcg
     7201 ataaatcgcg caagtttatc gcccagcaaa ttgttcgctg gctggagagc gagcatccac
     7261 tgaaagccaa aattttgccc actgaatggc tgggcgaaca tagcgcggag ttggtttctg
     7321 acgcggtgaa ttctttgctt gatgatatca gccgcgatcg tgcgcatcag atccgccatg
     7381 cgtttgatcg cgccaccttt gccctgatcg acaagctgaa aaacgatcct gaaatggcag
     7441 cgcgagccga tgccgtaaaa agctatctga aagaagacga agcttttaac cgctatctca
     7501 gtgaattgtg gggggattta cgggaatggc tgaaagtgga tatcaacagc gaagattctc
     7561 gtgtgaaaga acgtatcgca cgagcgggtc aatggtttgg cgaaacgtta attgccgatg
     7621 atgccttgcg ggcgtcgtta aatggtcatc tcgaacaagc cgcgcaccgc gtcgcgcctg
     7681 agttttccgc attcctgacg cgccacatca gcgatacggt aaaaagctgg gatgcgcagg
     7741 atatgtcgcg gcaaatcgag ttaaatatcg gcaaagatct gcagtttatc cgtgtcaacg
     7801 gtacgctggt tggcggttgt attgggctaa ttttgtattt gctgtcgcag ctcccggcct
     7861 tgtttcccct cggcaatttt tagaaatcca tgaaaaacta agtggctaac cgcgagagag
     7921 atcaaataaa ccatactaaa agaatggtaa taatgtggcc tcttttcatt atctcccgtg
     7981 gtacggggaa ggaaaatcat gtcacttgtc accgatctac ccgccatttt cgatcagttc
     8041 tctgaagctc gccagaaagg ctttctcacc gtcatggatc tcaaggagcg cggcattccg
     8101 ctggttggca cttactgcac ctttatgccg caagagatcc cgatggcagc cggtgcggtt
     8161 gtggtttcgc tctgttccac ctctgatgaa accattgaag aagccgagaa agatctgccg
     8221 cgcaacctct gcccgctgat taaaagcagc tacggctttg gcaaaaccga taaatgcccc
     8281 tacttctact tttctgatct ggtggtcggt gaaaccacct gcgatggcaa aaagaaaatg
     8341 tatgaataca tggcggagtt taagcccgtt catgtgatgc agttgccaaa cagtgttgag
     8401 gacgatgcct cacgcgcgtt atggaaagca gaaatgctgc gcttgcaaaa aacggtagaa
     8461 gaacgttttg ggcacgagat tagcgaagat gctctgcgcg atgccattgc gctgaaaaac
     8521 cgcgaacgtc gcgcactggc taatttttat catcttgggc agttaaatcc tccggcgctt
     8581 agcggcagcg acattctgaa agtggtttac ggcgcaacct tccggttcga taaagaggcg
     8641 ttgatcaatg aactggatgc aatgaccgcc cgcgttcgtc agcagtggga agaaggccag
     8701 cgactggacc cgcgtccacg cattttaatc accggctgcc cgattggcgg cgcagcagaa
     8761 aaagtggtgc gcgcgattga agagaatggc ggctgggttg tcggttatga aaactgcacc
     8821 ggggcgaaag cgaccgagca atgcgtggca gaaacgagcg atgtctacga cgcgctggcg
     8881 gataaatatc tggcgattgg ctgctcctgt gtttcgccga acgatcagcg cctgaaaatg
     8941 ctcagccaga tggtggagga atatcaggtc gatggcgtag ttgatgtgat tttgcaggcg
     9001 tgccatacct acgcggtgga atcgctggcg attaaacgtc atgtgcgcca gcagcacaac
     9061 attccttata tcgctattga aacagactac tccacctcgg atgtcgggca gctcagtacc
     9121 cgtgtcgcgg cctttattga gatgctgtaa ggagtggcag tggcatattc gattggcatt
     9181 gattccggct caaccgccac caaagggatc ttactggcag acggcgtgat tacgcgccgt
     9241 ttcctcgttc caacaccctt tcgcccggca acagcaatta ctgaagcctg ggaaactctg
     9301 cgcgaagggt tagaaacaac gccgtttctg acgctcaccg gctacggacg gcaactggtg
     9361 gattttgccg ataaacaggt gacggaaatc tcctgtcacg ggctgggcgc acggtttctt
     9421 gcgccagcaa cgcgtgcagt aatcgacatc ggtggtcagg acagcaaagt gattcagctt
     9481 gatgatgacg gtaacctgtg cgatttcctg atgaatgaca aatgcgcggc gggcaccggg
     9541 cgtttcctgg aagtgatctc gcgcacgctt ggcaccagcg tcgagcaact cgacagcatt
     9601 actgaaaatg tcagaccgca cgccatcacg agtatgtgca cagtgtttgc cgaatcagaa
     9661 gtgatcagcc tgcgctcagc gggcgtcgcg ccagaagcga ttctcgccgg agtgattaac
     9721 gcgatggcgc ggcggagtgc caatttcatt gctcgtctct cctgtgaagc gccgattctg
     9781 tttactggtg gcgttagcca ctgtcaaagg tttacccaca tgctggaaag ccatctggga
     9841 atgccggtac aaactcaccc tgatgcgcaa ttcgctggtg caattggcgc ggcggtgatc
     9901 ggtcagcggc agagaaaacg cgcatgaaag agtatctatt tttatttcac tccacggtcg
     9961 gcgtcataca aacccgcaag gcgttgcagg cagcgggcat gacttttcgc gtcagcgata
    10021 ttccccgtga tttacgcggc ggctgcgggt tatgcatttg gctgacctgt ccgcctggcg
    10081 aggaaattca atgggtgatc cctgggcata cggaatcagt ttattgtcag caggatggtg
    10141 gctggcgctg cattgcacat tacgggattt ctccgcgtta aggattcatt aagatcgcta
    10201 cgtatacttc tcgatatctt attgatttgc cttaaaggga ttaccgtgac aaaaagtatt
    10261 tcgttaagta aacgcatctt cgttattgtg attctttttg tcattgtggc agtgtgtact
    10321 ttcttcgtgc aatcttgcgc ccgtaaaagc aatcatgcag catcatttca gaattaccac
    10381 gccaccattg acggcaaaga aattgcaggc ataacgaata atatctcttc gctgacctgg
    10441 tcggcacaaa gcaatactct ttttagcact atcaacaaac cggccgccat tgttgaaatg
    10501 accaccaatg gcgatttgat ccgcacaatt cctctggatt ttgttaagga tctcgagacg
    10561 atcgaataca ttggcgataa tcagtttgtc attagcgatg agcgcgatta tgccatttat
    10621 gtgatttcac tgaccccaaa ctcggaagta aaaatcctca aaaaaataaa aatcccgctg
    10681 caggaatccc caaccaattg cggttttgaa ggattggcct attctcgcca ggatcacact
    10741 ttctggtttt ttaaagagaa aaacccgatt gaggtctaca aagttaacgg gctgttaagt
    10801 agcaatgaat tgcacatcag caaagacaag gcactacaac ggcaatttac tttagatgat
    10861 gtgtccggcg cagaatttaa ccaacaaaaa aatacgcttc tggtgctgtc tcatgaatct
    10921 cgcgcactcc aggaagtaac gctggctggc gaggttatcg gtgaaatgtc actaaccaaa
    10981 ggcagtcgag gtttgtcgca taacatcaaa caggccgaag gggttgcgat ggatgcctca
    11041 ggtaatattt atatcgttag cgaaccaaat cgtttttatc gctttacccc tcaatcatcg
    11101 cattaacagg ctggcatgtt tcaccttttt gttattaact tgttaacaac aaaaatgaca
    11161 aacaaggtta aacatgcctt cgccctcgca tcccgtagaa cgcttttctt tcagcaccgc
    11221 gcttttcggg atgctggttc tgaccttagg tatgggttta ggccgcttcc tttatacgcc
    11281 tatgttgccc gtcatgatgg cggaaggatc gttttcattt agccagctct cgtggattgc
    11341 cagcggcaac tatgcggggt atctggctgg cagtctgcta ttttcgtttg gcgcatttca
    11401 ccagccatcg cgcctgcgcc catttctgtt ggcttctgcc ctggcgagcg ggttgttgat
    11461 cctcgcaatg gcatggttgc tgccatttat tctggtgtta ttgattcgcg tcctggcggg
    11521 tgtcgccagc gccggaatgc tgatttttgg ttcgacgctg attatgcagc acacgcgcca
    11581 tccttttgtg ctggcagctt tgttttctgg cgttggcatt ggcatcgcac tgggcaatga
    11641 atatgttctg gcaggcctgc attttgacct ctcctcgcaa acgttatggc aaggcgccgg
    11701 ggcgctttct ggcatgatgc tgattgcact tacgctttta atgccctcga aaaaacacgc
    11761 catcacacca atgccattgg caaaaacgga gcaacagata atgagctggt ggttactggc
    11821 tattctgtac ggcctggcgg gatttggtta tatcatcgtc gcgacttacc tgccgctcat
    11881 ggcgaaagat gcaggctcac cattgttaac cgcccatctc tggacgttag tcggcttatc
    11941 aattgtgcct ggttgctttg gctggctatg ggcagcaaaa cgttgggggg ctttgccctg
    12001 cctgacggcg aatttgctgg tgcaggctat tagtgtgttg cttactctcg ccagcgactc
    12061 gcctctcttg cttatcatca gcagtattgg ttttggcagt acctttatgg gcacgacatc
    12121 tctggtgatg actatcgccc gccaactgag cgtaccggga aatctcaatc ttttaggctt
    12181 tgtgacgctc atttacggca ttgggcaaat cctcggcccg gcgctgacca gtatgctcgg
    12241 caatggaaca tcggcgcttg ccagcgccac tctctgcggc gcggcggcgt tgtttattgc
    12301 tgcgcttatc agcacagtgc aattatttaa gctacaagtg gtcacttcat gaggaaagtt
    12361 tcccttacca ctcaagaaac tcaacagcaa ccgttgatgt cagccagacc ccgttttccg
    12421 cttgccagaa agggatgcct cgtttcgcca tttccagtgc tttgacggtt aagataaccg
    12481 gcgatccgtg tcttgctccc actttgcgtg ccgttgcttc atcagcagag agatgtacgt
    12541 agtgacgctc acctgcaatc agcccctgtt tttttatctc gtcaaggaaa cggcttgccg
    12601 taccgtggta gagaaactgt ggcggtgttt tttcggcaaa ggaaatagcg acctgcgaag
    12661 tcgaatgccc ctgaaccgcg cggatgcacc taccatcact ggaataacta aaacgctttt
    12721 tatcgctggt ggcaaccaca gtatccagca atgcacgggt taaccgcttg cccgcttttt
    12781 gggcacaaag tatgagctta tcgatatccg cccatccttc acggtccagt acgataccaa
    12841 tcgcctccgg tttgtgtcgg aggacaaaac ttaaaaactt gctcgtatcc gccagctctt
    12901 tttcgttgta ctttgccatc tcgttctcgt ccctggattt tctgttttta tttacttatg
    12961 tcattgtcac tgggtttatg aatttactgc aactcaatcc agcgcagaac cttttcccta
    13021 tagcgtaatt tttccgctat gcaaaaaacg catagccaga aacatacttg cattttcccc
    13081 atacccacat ggctgataag gtttttcagc tccaggaatt ttcctggctc aagcaacaca
    13141 caaacacaaa aaaacaatgg ggattgttat gacacaacaa ggggatgctg tagccgggga
    13201 gctggcgaca gaaaaagtgg gcattaaagg gtatctggct ttttttctca ccataatctt
    13261 tttttccggt gttttttccg gaacagacag ctggtggcgc gtttttgatt tttccgttct
    13321 gaacggatcg tttgggcaac tgcctggtgc gaatggcgct accacctcgt ttcgcggcgc
    13381 aggtggcaca ggagctaaag atggctttct cttcgcactg gaactggctc cgtcagtgat
    13441 tctctctctg ggaattatct cgattaccga tggtcttggc ggattgcgcg ccgcgcagca
    13501 gttaatgacg cccgtgctaa aaccgttgtt gggcattcca ggcatctgtt cactggcgct
    13561 gattgctaac ctgcaaaaca ccgatgccgc cgcaggtatg accaaagaac tggcgcagga
    13621 aggggaaatt accgaacgtg ataaagtgat attcgccgcc tatcaaacca gcggtagcgc
    13681 catcatcacc aactacttct cttccggcgt cgcggtgttt gcctttctgg gcacgtccgt
    13741 aatcgtacct ttagccgtca tcctggtgtt taaatttgtc ggagccaaca ttttgcgcgt
    13801 ctggcttaac ttcgaagaac gtcgcaaccc aacgcaagga gcgcaagcat gacaactcag
    13861 gtccgcaaaa acgtcatgga tatgtttatt gatggcgcgc gccgtggctt tactatcgct
    13921 accaccaacc tgctgccaaa cgtggtgatg gcctttgtca tcatccaggc gctgaaaatt
    13981 actggcctgc tcgactgggt gggacatatc tgtgaacccg taatggcgct gtggggattg
    14041 ccaggtgaag cggcaaccgt ccttttagcc gcattgatga gcatgggcgg cgcagtcggt
    14101 gttgccgcca gtctggcaac cgctggcgca ttaaccggcc acgatgtcac cgttttactg
    14161 cccgcgatgt atttaatggg caacccggtg caaaatgtcg gtcgctgcct ggggacagcc
    14221 gaagtgaatg ccaaatatta tccgcatatc attacggtct gcgtgattaa cgcattgctg
    14281 tcgatctggg ttatgcagct tattgtttaa caaggagtta ccatgattga ttattccgca
    14341 gccggtttta ccctgctgca gggagcgcat ttgtatgcgc cggaagatcg gggaatttgc
    14401 gatgtcctca tcgctaacgg caaaattatt gccgttgcca gcaacatccc ttctgacatt
    14461 gtaccggact gcacggttgt cgatctcagc gggcagatcc tctgcccagg ttttattgat
    14521 caacacgtcc atttgattgg tggtggcggc gaagcaggtc ccacgacacg cacgccagaa
    14581 gtggcgttaa gtcgtctgac ggaagcgggc gtcacgtcag tggttggtct gctgggtacc
    14641 gactctatct ctcgccaccc ggaatccctg ctcgccaaga cccgtgcgct caatgaagaa
    14701 ggcatcagcg cctggatgct gaccggcgct tatcatgtcc cttcccgcac cattacaggt
    14761 tccgtggaaa aagacgtggc gattatcgat cgcgtgattg gtgtgaaatg cgccatctct
    14821 gatcaccgtt ctgccgcacc tgacgtttat cacctggcta atatggcggc ggaatcccgc
    14881 gttggcggtt tgcttggcgg taaacctggc gtcaccgtgt tccacatggg cgacagcaaa
    14941 aaggcgttac agcctgtcta tgacctgctg gaaaactgcg atgtgccgat cagcaagctg
    15001 ctgccgaccc acgttaaccg caacgttccg ttgtttgagc aggcgctgga gttcgcgcgc
    15061 aaaggcggca ccatcgacat caccagtagc attgacgaac cggtcgcccc tgccgaaggt
    15121 attgcccgcg ccgttcaggc gggtattccg ctggcacgcg tcaccctcag ctccgacggc
    15181 aacggtagcc agccgttctt tgatgacgaa ggaaatttaa cccatatcgg tgttgcaggt
    15241 tttgaaacat tgctggaaac cgtgcaaatg ttggtcaaag actatagctt cagccttagc
    15301 gatgccctgc gcccgctcac cagtagcgta gccggtttcc ttaacctgac cgggaaaggc
    15361 gaaattctgc caggcaatga cgcagactta ctggtcatga cgcctgaatt gcgcattgag
    15421 caggtatacg ctcgcggcaa actgatggtc aaagacggca aagcctgcgt gaaaggaacg
    15481 tttgaaacgg cataaccggt tgtcagccgc ccgaatggac gtagaatgcc cgcaagggcg
    15541 gctgacagag tataacgtaa tggatgactg tggtgcggtt ttgcataaca ttgaaaccaa
    15601 atggctttat gattttctga ccctggaaaa atgtcgcaat ttttcccagg cggcagtcag
    15661 tcgcaacgtc tcgcaaccgg cattcagccg ccgcatccgt gcactggaac aggcgattgg
    15721 cgttgaattg tttaaccgcc aggtgacgcc gctgcaactc tcggaacaag ggaaaatctt
    15781 ccattcgcag atccgccatc tgttgcaaca gttagaaagc aacctagcag agctgcgtgg
    15841 cggcagcgat tacgcgcaac gtaaaatcaa gatagccgct gcacactctc tttccctcgg
    15901 tctgttaccg tccattatca gccagatgcc gccgctcttt acctgggcaa ttgaagccat
    15961 tgatgtcgat gaagcggtcg ataaactgcg cgaagggcaa agtgactgta ttttttcctt
    16021 tcacgacgaa gatttgctgg aagcgccgtt tgaccacatc cgcttatttg aatctcaatt
    16081 gttccccgtc tgcgccagtg acgaacacgg agaagcactt tttaacctcg ctcagccaca
    16141 ctttccgtta ctgaattaca gccgcaactc ctacatgggg cgattgatta atcgcactct
    16201 gacgcgccac agtgagttga gtttcagcac cttttttgtc tcttcgatga gcgagctttt
    16261 aaagcaggtt gccctcgacg gatgtggaat tgcctggttg ccggagtatg ccatccaaca
    16321 agaaattcgc agcgggcagc tcgttgtact gaatcgggac gaactggtga tcccgattca
    16381 ggcctatgca taccggatga atacccgcat gaatccagtt gccgaacgct tctggcgtga
    16441 actgcgcgag ctggagattg aacctggttg aaagttttct gcagaaaatc ttccggcgct
    16501 gggaattatg attgaggttc ctgccgcagt catgattgca gacaaattag ccagtgaagt
    16561 cgatttcttt tccatcggca ccaacgatct gacgcaatat attatggcgg cagacagagg
    16621 aaatagcacc gtcgcgaaac tggtcgatta ttgtaatgac gcggtgatta acgctattgc
    16681 tatggtgtgt caggccggac gtaataatga aattccggtc agtatgtgcg gcgaaatggc
    16741 tggagatatc cagcaaacag cacgactgct caccatgggg attgataagc tcagtgccag
    16801 tccatcccgg cttccggcct tgaaagcagc aatcagaacc tcccactaat tgtgacctaa
    16861 cgcgatacgt cttgcgcgta tcgcgtttcc cgtcatttac tgcggcatcg tgcgatgcca
    16921 gctccccttc tgccagacca ggtcaaacgt tagcggctca tcatcgtggt acagcatcat
    16981 ctccagacta atgaccaact cgtggcaata gactgctttc agattgacct gcaatgccgc
    17041 aatacgtggc tcatgaaata aaagcgcgga acgtaactgg cggcaatacc aactctggag
    17101 aagcttgtcg ctctgattat gcccttgcca ctcaggcatg cctgaaacaa gtaatattgg
    17161 tagcggtgaa tggggagaac taaaaagttg aaagagatcc cggcatacgg aatcacgcag
    17221 cgataacgta ttgtgtcctg tactttcagg taataccgtc tgaactgatt ggcgcatcat
    17281 tttgcgcact cctgacgttt agtggcatct gtatcctgcc ctggctgccg gacaccactg
    17341 ggttgatgct catattccat aatcattcct ttatgctcat tgcgtccctg gcactttcat
    17401 aagggattta taagatccgc caaagataaa acactgtaca gaaaagaata tccactgact
    17461 ttttatttaa aaaggtatca ttt
//
LOCUS       DAPHCV010000101        17396 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.101, whole genome shotgun sequence.
ACCESSION   DAPHCV010000101 DAPHCV010000000
VERSION     DAPHCV010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.101"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            71..589
                     /locus_tag="RH033_003557"
     CDS             71..589
                     /locus_tag="RH033_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDV2992686.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNAQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            605..1144
                     /locus_tag="RH033_003558"
     CDS             605..1144
                     /locus_tag="RH033_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HDV2992687.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1237..2814)
                     /gene="guaA"
                     /locus_tag="RH033_003559"
     CDS             complement(1237..2814)
                     /gene="guaA"
                     /locus_tag="RH033_003559"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDV2992688.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2883..4349)
                     /gene="guaB"
                     /locus_tag="RH033_003560"
     CDS             complement(2883..4349)
                     /gene="guaB"
                     /locus_tag="RH033_003560"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDV2992689.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4511..5887
                     /gene="xseA"
                     /locus_tag="RH033_003561"
     CDS             4511..5887
                     /gene="xseA"
                     /locus_tag="RH033_003561"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDV2992690.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDNAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQLKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANLRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPIVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     THQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPRIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTSAADGAVLKQVKQVKVGETLTTRLGDGVVISEVSAVTKTRK
                     SRKKTSNP"
     gene            complement(5956..14007)
                     /locus_tag="RH033_003562"
     CDS             complement(5956..14007)
                     /locus_tag="RH033_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RatA-like protein"
                     /protein_id="HDV2992691.1"
                     /translation="MSLTLNKILVLCALLISAMLPGWSWAESAWQDSSDTVGEFNGTV
                     PTADSASIPVYQGSVFLDPAKTHEVAFTAKPSEFSADVSVSKLLVTNPQDREGDIIAT
                     PRWENQTPPTVSLVWADAATPDTLLDPQPVADRSFCAQGLAGRSLIAWAQPDPQQTMP
                     LLYLLTSTGYPYESVLTLADQKVTLKIAPAQGDLISVSAAGYDESSGAAKTTVGGSIT
                     LTVTTKDCVGNVVGNIPFVIKRKDAENRQGVVNNTAPVKLGTTELTTTATEYRGTTDA
                     NGVATVTVTQANGPGVKTPLVASLAGIAQTSETAVIFTVLTSPDVPQATMWGHMPDTL
                     KARDYTFSRPKLAAEVDNEDGTVNDHNETWSTFTWSGADKHCDILPGMRQFGALATVV
                     PTSVQDVAGWPMQGNYYWSSLAGMSGQHYAADVSNRSEAQKPDDTTFIVSCVDKEAPD
                     VEPKLVLTPGSYDSTIKAMKVKVGEEASLRLTITDSKNNDQPLAYYYFSLHLDDGVNR
                     KNQTDAAWETHPVQIDGGSNVRKVDAHTYEGITDANGEASLTLTQPGGVGVKTHITAK
                     MRSDFTASDEKDVIFTVITSPDSDKARMWGHMLGIIEANNIFKRPRLADETDNELGAV
                     RENNEDWALFDQNSSMQAECGLGHIPSQSSLHSLFAAHPANVIGTEYGWPTLQKAYLS
                     AVEETSHASVNLATGNIDTYSGFKQNYLSCSGNEMVAQIAATTDHDVSTSSRAQAKVG
                     ETITMTVRTFNALNNAPVPYTAFTITKDMGKNRQGQTTGFDDPTRGAIEMNGTLYGTS
                     QPSLVYAGTTDAQGFATVEIKQPQGVGLATPLNIAPVNSYIPNTVNYNVIFTTLTSPD
                     AVGAQMWGHMDETITVDALTFARPRLAAEVSSPDGTLTENNEVWSRVSQANTSSTSKG
                     GCGANMLPRRSQLNALYDANSGNAVQTVHGWPTQRQPYWSSSPADQVPHYYTIALNDG
                     ARTVGGSTAVYVSCLTTANNPASSITLEVVDPAQWNAAANAAKLKKGETLQVKVTVKD
                     AQGNPLGDMPFTLKRGDGYTRSNEKHIAGGGDALVAPVVINSGLADETSLNDTAAAYS
                     AMTGSDGTKILTITRPDTHGTKTSLTAALYSDTTKKATLDTIFTVVTSPDSDKAKMWG
                     HMPETVTAADGTVFKRPLLLKELSSTSGRTAIAEENEDWAQFTQTQAISTSSNGCGSE
                     YVPSQAGLESLYEANRGNAMKTVQGWPVASSYLSSTTGSSSLEQRDFKAVNLSSGTSS
                     IIPSATKELLTCQTTPIVKASQIVLEAADPTKFDSTNNVVKAKKGEEAVLRVTTKDAQ
                     GNPVGNTAFTLKRNTSVNRANVSTTTSIASLAVTDAWGNTQDDFLSTTLVIYGVTGAD
                     GITTFTLKQDQTTGLKTELTAALDSSSSTKSTLPVVFTVLTSPDSPKAKFWGHMAETA
                     TGDDGLIYRRPLLRDENSATTSIGTLVEEGEAWSTFPSGQANDTSINGCGAEYVPTDN
                     ELRAIYAHQGSSALHDAIGWPVSRFYISNTVADTFTQTFTYDVVSLKTGDETQMPSSG
                     GALLSCRTTPVAVASQIIVEANDTAQFVKVDDTLSALKVKKGEDAVIRVVTKNAQGNS
                     VPNVPFILRREGSKNRQNAEMINKSITVINAAGASARMNSSSSLLYGVTGADGTTSFT
                     VKQDDSMGLVTNMYAQLYQPTIESNKLPVMFTVITSPDTPLASYWGHMPETFTTRSGI
                     AFKRPLLTAEHPAGQSTMANNESWLSLNTAAKNDVSKSDCGEPYQPLLSEFQELYSEH
                     PNGAIGTDLGLPLTNTWWAYDKIAYANVWYDQSINLSNGSSSRALSNTVAFVSCLVNP
                     HAVAASIEMTSTALDAEKTASNDGRPSATAAKGTAIRMTVIVRDSAGNLLPGANFNLI
                     RGTALDRAKNRLDSTYDDLTIVPVTPAGVNMSLYNNGAQALLTTGSDGKATFDVTQNE
                     TYGLATPLTATLMRDTTKSATMDVIFTVITSPNSPKAKYWGHMPDTFTSRAGVTFKRP
                     LLAAEATLGSSVSNNNESWSYLFYTNKVTPDCPVEYQPRLNELQGLYNDHPSGTILTD
                     LGLPITAGSGNWWTYEMSTTDALTWYYGVINLKTGQSTTTINGYALMLCLTQPHSAPA
                     SLTLSSTAYDEGRTASNGGTPTSSVKKGEMLPIVVTIKDANGNPVGGEGVTLKRVQAK
                     SRSGISVSSNTVDDLILDEVTPTSARISFNQNTSAWSGFTGSDGTITFNVTQNNTVGL
                     VTPFTASLARNPQVTANQDLIFTVVTSPDSAKANYWGHMPATLTAVNGAVFERPKLWS
                     ELTSTSGVGKINNNNEDWPYFTPTQKSDASVSPCEVARQPLFNDLSSLSARYPNNTFV
                     TETGWPAYYTWWAEDKSADGKDQSVDLRNGTLYTGSTKSFQPCLANARSTVSSVTLTS
                     TAFDAATQAAKVKKGEAMPVTVTVKDSAGNTVPNVEFTLKRGEASPRNAGATLYGDVV
                     AMDDLIVQPLSGSAVTLSESGNTISGMTGADGTASFTLRQDNTPGYKTPLTVTLANYA
                     SATDTLDAIFTVPTSPNVSSAHFWGHMADTVVVNGKSLHRPLLTTELPSGANPVSSPI
                     INYENWASAHIIDASKWDIARQCGSIENAPTYNELELLHTVFNSLGWPSSPSFPYLSS
                     QQCGMDEGTGAQDCSITLINKPGLVTCFQ"
     gene            complement(14128..15105)
                     /locus_tag="RH033_003563"
     CDS             complement(14128..15105)
                     /locus_tag="RH033_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992692.1"
                     /translation="MKQDKRRGLTRIALALALAGYCVAPVALAEESAWVDSGETNIFQ
                     GTIPWLYSEGGSATTDADHVTLTSDLKGARPTGSETDKRLYSGDKLTVSWEIGDTEGD
                     VDLGGLGDNAKTIDTIRWMSYKDAQGGDPKELATKVTSYTLTDDDRGRYIGIEITPTT
                     QTGTPNVGTALHLYDISTASGGGSDSDNVAPGPVVNQNLKVAIFVDGTSINLINGSTP
                     IELGKTYVAKLYSDENKNGKFDAGTDADVTANYDFRWVLSGSSQQLGTSGGIVNSSFD
                     NNNLAIPATNDEARTNLNGPARDGKEALTIPTNGDGVQGYKLHIIYKHK"
     gene            complement(15160..17337)
                     /gene="sinH"
                     /locus_tag="RH033_003564"
     CDS             complement(15160..17337)
                     /gene="sinH"
                     /locus_tag="RH033_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like inverse autotransporter SinH"
                     /protein_id="HDV2992693.1"
                     /translation="MLRWKRCIILTFISGAAFAAPEINVKQNESLPDLGSQAAQQDEQ
                     TNKGKSLKERGADYIINSATQGFENLTPEALESQARSYLQSQITSTAQSYIEDTLSPY
                     GKVRSNLSIGQGGDLDGSSIDYFVPWYDNQTTVYFSQFSAQRKEDRTIGNIGLGVRYN
                     FDEWLLGGNIFYDYDFTRGHRRLGLGGEAWTDYLKFSGNYYHPLSDWKDSEDFDFYEE
                     RPARGWDIRAEAWLPSYPQLGGKVVFEQYYGDEVALFGTDNLEKDPYAVTLGLNYQPV
                     PLLTVGADYKAGTGDNSDVSINATLNYQFGVPLKDQLDSDKVKAAHSLMGSRHDFVER
                     NNFIVLEYKEKDPLDVTLWLKADATNEHPECVIKDTPEAAVGLEKCKWTINALINHHY
                     KIVAASWQAKNNAARTLVMPVIKENTLTEGNNNHWNLVLPAWQYSSDKAEQEKLNTWR
                     VRLALEDEKGNRQNSGVVEITVQQDRKIELIVNNIANVPEENNHSHEASAQADGVDGV
                     VMDLDVTDSFGDNTDRNGDALPEDNLTPRLYDAQDKRVTLTNKPCSTDNPCVFIAKQD
                     KEKGTVTLSSTLPGTYRWKAKAAPYDDSNYVDVTFLGAEIGGLNAFIYRVGAAKPSNL
                     IGKDKEPLPLNNTYRFVLWRDSNKDGVFQQVEKLTDEEMAQYDYKWEFTGKSINGEVG
                     AQANTSNEDIVIPATNREAAQTYGAQAGDGLQGYGLRVLYTKK"
BASE COUNT         4095 a         4620 c         4308 g         4373 t
ORIGIN      
        1 caatacattt actttcctct tttgatgatc ttaaatgtct tatttttcgt aatgtgtata
       61 acaaggaata gtgatgaaat ttaaaaaatg tcttctgcct gtggcaatgt tagcgtcatt
      121 cactctggca ggatgccagt caaatgctga tgatcatgcc gccgatgttt atcaaaccga
      181 tcaactgaat accaaacaag aaactaaaac cgttaatatt atttccattc ttcccgcaaa
      241 agttgccgta gacaacgccc aaaataaacg gaacgcacaa gccttcggcg cgcttattgg
      301 cgctgtcgct ggcggtgtta tcggccacaa cgtcgggtct ggcagcaatt ccggaacgac
      361 ggcaggtgca gttggcggcg gagctgtagg cgcggcagcg ggttctatgg tgaatgataa
      421 aaccttagtg gaaggtgttt ctttaacata taaggaaggc accaaagtgt atacctccac
      481 ccaggtgggt aaagagtgcc agtttacgac aggtttagcc gttgttatta ccacgacgta
      541 taacgaaaca cgtattcagc caaataccaa atgtcctgaa aagagctaat aatcaggagg
      601 agtcatgaag aaagtttttc tttgcgccat cttagcctcc ttaagctatc cggctatcgc
      661 ctcatcattg caggatcaac tctcggctgt cgcagaagcg gaacagcaag gtaaaaatga
      721 agagcaaagg cagcatgacg aatgggtcgc ggagcgcaac agggaaatcc agcaagagaa
      781 gcaacgtcgc gcaaacgccc aggccgcggc taataaaaga gcggcaacgg cagcggcgaa
      841 taagaaagct cgtcaggata aactggacgc cgaagccact gcggacaaaa aacgcgatca
      901 aagttatgaa gatgagctac gcagcttaga gattcagaaa caaaaactgg cgctggcgaa
      961 agaagaagcc cgcgttaagc gagaaaacga atttatcgat caggaactga agcacaaagc
     1021 tgcgcaaacc gatgtggtgc aatctgaagc tgacgcaaac aggaatatga ctgaaggcgg
     1081 tcgcgatctg atgaaaagcg tgggcaaagc agaagagaat aaatcagata gctggtttaa
     1141 ctaaacgatg ttagtaactt caaacctata attctttagg ataaaaaacc ctctgtagta
     1201 acagagggtt ttgttcattc atagtgcagg gtcaaatcat tcccactcaa tagttgctgg
     1261 tggcttgccg ctgatgtcat acaccacgcg ggaaatacca ttcacttcat tgataatgcg
     1321 gttggaaacg cgaccgagga aatcgtacgg cagatgtgcc cagtgtgcgg tcataaagtc
     1381 gatggtttcg acagcacgca gagagacaac ccagtcatac ttacgaccat cgcccattac
     1441 gccaacggaa cgtactggca ggaagacggt gaatgcctgg ctgactttgt cgtacaggtc
     1501 cgctttacgc agttcttcaa tgaagatggc gtcagcacgg cgcagcaggt cacaatactc
     1561 tttcttcact tctcccagaa cacgaacgcc aaggcctggt cccgggaacg ggtgacggta
     1621 caacatgtca tacggtaggc ccagctccag accaatctta cgcacttcgt ctttgaacag
     1681 ctctttcagc ggttcaacca agcccatctt catctctttc ggcaggccac ccacgttgtg
     1741 gtgagatttg atgacgtgtg ctttaccggt tgcagaagca gcagattcga taacgtcagg
     1801 gtagatagtg ccctgcgcca gccacttcac gtcttccagt ttcagcgctt cttcatcgaa
     1861 tacttcaacg aaaacgcgcc cgatgatttt acgttttgct tccggatcat tttcgccagc
     1921 cagcgctgac aagaagcgat cttctgccgg tacgtgaaca atattcagac caaagtgatc
     1981 gccaaacata tccagaacct gctctgcttc gttgagacgc agcaggccgt tgtcgacgaa
     2041 tacgcaagtc aggtttttac cgatagcgcg gtgcagcagc attgcagtca cggaggaatc
     2101 cacgccacca gagaggccga ggatcacttt atcgtcgcct acctgctcgc ggatacgggc
     2161 tacagcatcg tcgataattt tcgctggcgt ccacagggct tcacactggc agatatcacg
     2221 cacaaaacgc tccagcatgc gcataccctg gcgggtgtga gtcacttccg ggtggaactg
     2281 tacgccatag aagcgttttt cttcgttagc cataatggca aacgggcagc tttcggtgct
     2341 ggctacggtg acgaagtcgg acgggatagc ggtaacttta tcgccgtggc tcatccagac
     2401 atcgagcaga ggtttaccgt ctgcggtcag cgcatcttcg ataccgcgaa ccagtgcgct
     2461 gtcgtttacg acttcaacct gcgcgtagcc aaattcacgt tcgttagagg cttcaacgtg
     2521 accgcccaac tgcatcgcca tggtctgcat gccatagcaa acgccgaata ccggtacgcc
     2581 tgcttcaaaa acatactgcg gcgcacgcgg gctgttttct tcagtggtac tttccgggcc
     2641 gccggaaaga ataatgccgc ttgggttgaa gtcacgaatt tgtgcttctg tcacatccca
     2701 cgcccacagt tcgcagtaaa cacccagttc acgcacgcgg cgcgcaacca gttgagtgta
     2761 ctgagaaccg aagtccagaa tgaggatgcg atgcttatga atgttttccg tcattgacgc
     2821 ttattccgag gcaagtgaaa cagataatat aaatcgcccg acatgaagtc gggcgaagag
     2881 aatcaggagc ccagacggta gttcggggac tctttagtaa tggtcacgtc gtgaacgtgg
     2941 ctttcctgaa tgcccgcacc gctgatacgt acaaactccg ctttagtacg cagttcgtcg
     3001 atagtaccac agccggtcag gcccatacag gagcgcaggc cgcccatctg ctggtgaatg
     3061 atctctttca ggcgaccttt ataggccacg cgaccttcga taccttccgg caccagtttg
     3121 tcggcagcgt tgtcactctg gaagtaacgg tcagaagaac ctttggacat cgcgcccagg
     3181 gaacccatac cacggtaaga tttgtaagaa cggccctggt agagttcaat ttcacccgga
     3241 gattcttcag tacctgccag catggaaccc accattaccg cgcttgcgcc agcggcgata
     3301 gctttggcga tgtcgccaga gaaacggata ccgccgtcag caataacagg aataccggtg
     3361 ccttccaggg cttctactgc atcagcaacg gcagtgatct gcggaacacc gacgccagtc
     3421 acgatacgag tcgtacagat agagcctggg ccgataccca ctttaaccgc gctgcaacct
     3481 gcttctgcca gagcgcgggc gcccgcagct gttgccacat taccgccgat gatttgcaga
     3541 tccggatatt tagcacgagt ttcacggata cgctgcagaa cgccttcaga gtgaccgtga
     3601 gaggagtcga tcagcagaac gtcaacgcct gcggcaacca gcgcgtcgac acgctcttcg
     3661 ttaccagcgc cagcgccaac cgctgcacca acacgcagac ggccttgctc gtctttacag
     3721 gcgttcggtt tacgttccgc tttctggaag tctttcacag tgatcatgcc gatcaggtgg
     3781 aattcgtcat caaccaccag cgccttttca acgcgttttt cgtgcatttt tgccagcacc
     3841 acttcacggg cttcgccttc acgcacggtt accagacgct ctttcggcgt catgtaaacg
     3901 ctaaccggtt gattcagatc ggtcacaaaa cgcacgtcac gaccggtgat gatgccgacc
     3961 agttcgtttt cttcggtaac caccgggtaa cccgcaaagc cgttacgttc ggtcagttct
     4021 ttcacttcac gcagggtggt tgtcggcagc acggtctgcg gatcagttac cacgccagat
     4081 tcgtgttttt tcacacggcg aacttcttct gcctggcgtt caatggacat gtttttgtgg
     4141 ataaagccga taccgccttc ctgagccaga gcaatagcca ggcgcgcttc cgttacggta
     4201 tccattgctg cggaaagcat agggatattc agacgaatag ttttcgtcag ctgggtgctg
     4261 aggtcagcag tattcggcag aacggtagaa tgtgcaggaa cgaggagaac gtcgtcaaac
     4321 gtcagagctt ctttagcgat acgtagcatg ggcaatatct cgaccagagt ggttaataaa
     4381 tattgccgcg gcattataca gagcgtaacc gattgcatct accccttttt gcaaaaaatg
     4441 cttgctatcc ccggagggcg ggttactatc gactgaataa cctgctgatt tagaatttga
     4501 tctcgctcac atgttacctt ctcaatcccc tgcaattttt accgttagcc gcctgaatca
     4561 aacggttcgt ctgctgcttg agcatgagat ggggcaggtg tggatcagcg gcgaaatctc
     4621 taatttcaca caaccggctt ccggtcactg gtactttacg cttaaagacg ataacgccca
     4681 ggtgcgctgc gcgatgttcc gcaacagcaa tcgccgggtc accttccgcc cgcagcatgg
     4741 gcaacaggtt ttagttcgcg ccaacattac gctgtatgaa ccgcgcggcg attatcaaat
     4801 tattgtcgaa agtatgcagc ccgccggtga aggtttactg caacagaagt acgaacagct
     4861 taaagcgaag ttgcaggctg aaggtttgtt cgatcagcaa cttaaaaaac cactcccctc
     4921 ccctgcacac tgcgttgggg tgattacctc aaaaacaggt gccgcactgc acgatatttt
     4981 gcatgtgttg aaacgccgcg atccgtctct gccagtgata atctatccca ctgccgttca
     5041 gggcgatgac gcaccagggc aaatcgttcg cgccattgag ctggcgaatc ttcgcaacga
     5101 gtgcgacgta ttaatcgtcg ggcgtggcgg tggctcgctg gaagatttat ggagttttaa
     5161 tgatgagcgt gtggcgcggg cgatttttgc cagtcgcatt ccgatagtca gcgccgtcgg
     5221 gcatgaaacc gatgtgacaa ttgctgactt tgttgctgat ctacgtgcgc caacgccgtc
     5281 agccgccgct gaagtggtga gccgaaatca gcaagaattg ctacgtcagg tgcaatcgac
     5341 ccatcaacgg ctggaaatgg cgatggatta ttatctcgcc aaccgcacgc gtcgttttac
     5401 gcagattcat catcgcttgc agcaacagca tccgcagctc cggctggcac gtcagcaaac
     5461 tatgctcgaa cgtctgcaaa agcggatgag ctttgcgctg gaaaatcagc ttaagcgtgc
     5521 cgggcaacag cagcagcgat taacacagag gctaaaccag caaaatccgc agccgcgtat
     5581 tcaccgcgca cagacgcgaa ttcaacaact ggaatatcgt ttagcagaaa ccctgcgcgc
     5641 acagcttagc gccacgcgtg aacgtttcgg taatgcagta acacacctcg aagccgtaag
     5701 cccactgtca acgctggcgc gcggttatag cgtcaccagc gccgctgatg gcgcggtgtt
     5761 aaaacaggtt aagcaagtga aagtgggtga gacactgacc acacgactgg gagatggtgt
     5821 ggtgattagt gaagtcagcg ccgtaacaaa aacccgcaaa tcacgtaaaa aaacgtctaa
     5881 tccgtagacc ggataagagg cgccagcatc gcatccggca ataactccca ggccacgcca
     5941 gatccgccat gctggttact ggaagcaggt caccagaccc ggtttattta ttagcgtgat
     6001 actacaatcc tgtgctccgg taccttcgtc catcccacat tgttgagagg acaaataagg
     6061 aaaactgggt gaagacggcc atcccagtga attaaaaaca gtatgcaaca actctaattc
     6121 attgtatgtc ggtgcatttt ctatgctgcc acactgcctt gcgatatccc atttactggc
     6181 atcaatgata tgtgcagaag cccagttttc gtagttaata atcggtgagg atacgggatt
     6241 agccccggac ggcaattctg tcgttaacaa cggtcgatgt agcgatttac cattcaccac
     6301 taccgtgtct gccatatgtc cccaaaagtg cgccgaggaa acatttgggc tggttggtac
     6361 cgtaaaaata gcgtccagcg tgtctgtcgc ggaagcgtaa ttagccagag taaccgtcag
     6421 tggcgtttta taaccagggg tattatcctg gcggagcgta aaacttgccg tcccatccgc
     6481 gcctgtcatg ccactaatgg tattaccgct ttcactcagc gtaacagccg aaccagataa
     6541 cggctgaacg atgagatcgt ccatcgccac cacatcacca tacaacgttg ccccagcgtt
     6601 acgcggcgat gcctcaccgc gtttcaaagt aaattccaca ttcggcaccg tattcccggc
     6661 gctatctttc accgtcacgg taacgggcat ggcttcgcct tttttcactt tcgccgcctg
     6721 agtggctgca tcaaacgctg ttgaggtaag tgtgacgctg gaaaccgttg aacgcgcgtt
     6781 agccaggcaa ggctgaaatg attttgttga acccgtatac agcgtgccat ttctcaaatc
     6841 aaccgactgg tctttaccat cggcggattt atcctctgcc caccaagtgt aatacgcagg
     6901 ccagccagtt tcagtgacaa acgtgttatt cggataacgc gcagacaacg aacttaaatc
     6961 attgaatagt ggctgccttg ctacttcaca agggctcaca ctcgcatctg atttctgtgt
     7021 tggggtaaag tacggccagt cttcattatt gttattgatc ttgccgacgc cagaagtcga
     7081 tgtcagttca ctccacaatt tcgggcgttc aaataccgca ccattcactg ccgtcaaggt
     7141 tgctggcata tgcccccagt agttagcctt cgcagaatcc gggctggtca ctaccgtgaa
     7201 tatcaggtcc tgattagccg taacctgcgg atttcgcgcc aatgaagctg taaacggcgt
     7261 taccagaccg acggtgttat tttgggtaac gttgaacgtt atcgtgccat cactgccggt
     7321 gaagccgctc catgccgacg tattctggtt gaaggagata cgcgccgatg tcggggtgac
     7381 ttcatctaaa atgagatcat ctacggtatt ggaggataca gagatccctg aacggctttt
     7441 cgcctgcacg cgcttgagcg taacaccttc gccgccaaca ggattaccat tggcatcttt
     7501 gatcgtcacc acgatgggaa gcatctcacc ttttttcaca gatgacgtgg gggtaccacc
     7561 attgctggca gttcggccct catcataggc agtcgatgac agcgtaaggc ttgctggtgc
     7621 cgaatgtggc tgcgtcagac agagcattaa agcgtatcca ttaatagtcg tcgtcgattg
     7681 tcccgttttc aaattaatga cgccgtaata ccacgtcaac gcgtcagtgg tgctcatttc
     7741 ataagtccac caatttccac ttccggcagt tataggcaac cccaaatctg tcagtattgt
     7801 cccgcttgga tgatcattat atagcccctg taactcattg agacgcggct gatactcaac
     7861 cgggcaatcc ggcgtaactt tattggtata aaaaaggtat gaccacgact cattattatt
     7921 acttaccgag gaccccaagg tcgcttctgc tgccagtagc ggtcgtttaa aggtcacccc
     7981 cgccctactg gtaaacgtgt cgggcatatg accccagtat tttgctttcg gtgagttcgg
     8041 actggttatc acagtgaaaa taacatccat cgtggcactt tttgtggtgt cgcgcatcaa
     8101 tgttgcagtg agcggagttg ccagcccata agtctcgttt tgcgtcacat caaaagtcgc
     8161 tttaccatca cttcctgtcg tcagaagtgc ctgtgcccca ttattataga gtgacatgtt
     8221 tactccggca ggggtgaccg gcacgatagt aagatcatca tacgtagaat ctagcctgtt
     8281 ttttgcacga tccaacgcag tcccgcgtat gagattaaag tttgcgcctg gcaacagatt
     8341 tcctgcgcta tcccgcacaa tcaccgtcat ccggatcgcc gtacctttcg ccgccgttgc
     8401 actagggcga ccatcgttgc tggccgtttt ctccgcatcc agtgccgtgg aggtcatctc
     8461 tatagaagct gccactgcat gagggtttac cagacaggac acaaatgcca cggtgtttga
     8521 taatgccctg gagcttgatc cattcgataa attaatagac tgatcgtacc agacgttcgc
     8581 gtaggcaatt ttatcgtaag cccaccaggt gttagtcagc ggaaggccta aatcggtccc
     8641 tatcgcacca ttaggatgtt cactgtataa ctcttgaaat tcgcttaata acggttgata
     8701 aggttcgccg cagtctgatt tgctgacatc attttttgct gccgtattca aagaaagcca
     8761 tgattcatta ttagccatgg ttgactgccc cgctggatgc tcggcagtaa gcaatggccg
     8821 cttaaaggca attcccgacc gggtagtaaa cgtttcaggc atatgccccc agtaacttgc
     8881 cagtggcgta tccgggctgg ttatgacggt aaacatgacc ggtaatttgt tggattctat
     8941 cgtcggctga tacaactggg cgtacatatt ggttaccagg cccatagaat catcttgttt
     9001 taccgtaaag gaggtagtcc catccgcgcc agtcacccca taaagcaggc ttgaagaact
     9061 attcatcctc gcagaagcgc ctgccgcgtt aattaccgta atcgacttat ttatcatctc
     9121 ggcattttga cggttcttcg agccttcacg tctgagaata aaggggacat tgggcacgct
     9181 atttccctgc gcatttttag tcaccacgcg aatcaccgcg tcttcgcctt ttttcacttt
     9241 caacgcactc aaggtgtcgt caactttgac aaattgcgcc gtatcattcg cctcaacgat
     9301 gatctgactg gcaacggcga caggcgtggt ccggcagctc agtaatgcac caccagatga
     9361 gggcatctgc gtttcatctc ctgtcttcag cgaaacgaca tcatacgtaa aggtttgagt
     9421 gaatgtatct gcaacggtat tcgagatata gaacctcgac actggccagc caatagcatc
     9481 atgtaatgcg ctactcccct ggtgcgcata aatggcccta agttcattgt cagttggaac
     9541 atactccgcg ccacaaccat ttatcgaggt atcgttagcc tggcccgacg ggaacgtaga
     9601 ccaggcctcc ccctcttcca ccagggtacc gatactggtg gtagcactat tctcgtccct
     9661 cagtaaaggc cttcgataaa taagaccgtc atcgcctgtc gccgtttcag ccatatgccc
     9721 ccagaatttg gcttttggcg aatccgggct ggtaagcacc gtaaagacaa cgggaagcgt
     9781 cgatttagtg ctgctggagg aatccaacgc tgccgtgagt tctgttttta gcccggtagt
     9841 ctggtcttgc ttcaatgtga aagtggtgat gccatcagcc cccgtcacgc cataaataac
     9901 cagggtcgtt gaaagaaaat catcttgagt atttccccag gcatccgtta ccgccagcga
     9961 agcaatcgac gtcgtagttg acacattggc cctgttcacg ctggtgtttc gtttgagggt
    10021 aaatgcggta tttcccacag ggttaccctg cgcatcttta gtggtcactc gtaatacggc
    10081 ttcttcgcct tttttcgctt tgacaacatt attcgtactg tcaaattttg tggggtctgc
    10141 agcttcaaga acaatttggc ttgccttaac aatcggcgtc gtctggcagg taagcagttc
    10201 ttttgtggca gatggaatta tcgaactggt gcctgaactg agattgacgg ccttgaagtc
    10261 acgctgttcc aggctactac tacctgtcgt gctggaaagg taacttgagg cgacgggcca
    10321 gccctgaacc gttttcatcg cattgccgcg gttggcttca tacaatgact ccagcccggc
    10381 ctgggaggga acatattcac taccacaacc attagaggat gtgcttatag cctgggtttg
    10441 ggtaaattgc gcccagtctt cattctcttc tgcaatcgct gttcgacccg acgtactgga
    10501 taactctttc aacaataatg ggcgtttaaa gaccgttcca tctgcagccg tcactgtttc
    10561 aggcatatgc ccccacattt tcgccttatc gctgtccggg ctggtgacaa cggtaaaaat
    10621 ggtatcgagg gtcgcttttt tcgttgtgtc tgaatagagc gcggcggtca gcgacgtttt
    10681 tgtgccatgg gtatccgggc gggtaatagt gagaatttta gtgccatcgc tgccggtcat
    10741 cgcgctgtat gctgcggcgg tatcattgag cgaagtttca tccgccaggc cactattgat
    10801 cacaaccggc gcaaccagcg catcaccgcc accggcaatg tgtttttcgt ttgatcgggt
    10861 ataaccatcg ccacgcttca gcgtaaaagg catatcaccc aatggattgc cctgagcatc
    10921 ttttaccgtc accttcacct gtagcgtttc gccttttttc agcttcgccg catttgctgc
    10981 cgcgttccac tgcgccgggt ccaccacttc cagcgtaatg ctgctggcag gattgttagc
    11041 cgtcgtcagg caactgacat aaaccgccgt gctaccgcca acggtccgcg cgccatcgtt
    11101 gagggctatg gtgtagtaat gcggcacctg atccgcgggc gaactgctcc agtagggctg
    11161 acgctgcgtt ggccaaccgt gtaccgtttg aacggcgtta ccgctgttag catcatagag
    11221 cgcgttaagc tgggaacggc gtgggagcat attggcacca cagccacctt tactggtgct
    11281 ggaagtgttg gcctggctaa cgcgcgacca cacttcgtta ttttccgtta atgtgccgtc
    11341 cgggctggac acttccgcag ccagccgggg tcgggcaaag gttaatgcat cgacggtaat
    11401 cgtctcatcc atatgacccc acatctgcgc gccaaccgca tccggactgg tgagcgtagt
    11461 gaaaatcaca ttgtaattaa cagtattggg aatataggag tttacaggcg caatattcag
    11521 cggcgtcgct aagcccacac cttgcggttg cttaatttcc acagtagcaa aaccctgcgc
    11581 atccgtagta ccggcataga ctaaagacgg ttgcgatgtt ccgtagagtg tgccattcat
    11641 ctcgattgcg ccgcgggttg gatcatcaaa acctgtagtc tgcccctgac gatttttccc
    11701 catatcttta gtgatggtga acgcggtata cggcacgggc gcattattca aggcattaaa
    11761 ggtgcgtacc gtcatggtaa tcgtttcacc cactttagcc tgcgctctgc tgcttgtgga
    11821 aacatcatga tcggtggtcg cagcaatctg ggcgaccatc tcattgcccg aacaagaaag
    11881 gtagttttgc ttaaagccgg aataggtgtc gatgttgccc gtggccaggt tcaccgacgc
    11941 gtgcgacgtc tcttccaccg cactaagata cgctttttgc aacgttggcc agccatattc
    12001 ggtgccgatg acattggctg gatgtgcggc aaacaaactg tgcaatgagc tttggctcgg
    12061 aatatgacct aaaccacact cagcctgcat actgctattc tgatcaaaca gcgcccagtc
    12121 ttcgttattt tcccgcactg ctcccagttc gttgtcggtt tcatccgcca gccgtgggcg
    12181 tttaaagata ttattcgctt caataatgcc taacatatgt ccccacatcc gcgctttgtc
    12241 ggaatcaggg ctggtgatca ccgtaaaaat aacgtctttc tcatccgagg cggtaaaatc
    12301 actgcgcatt ttcgcggtga tgtgtgtttt cacccccacg ccgcctggct gggtcagcgt
    12361 cagagaggcc tctccgttag catcagtgat cccttcatag gtatgcgcgt caactttgcg
    12421 aacgttactg ccaccatcaa tttgtaccgg gtgcgtctcc cacgcagcgt cagtttgatt
    12481 cttacggtta acaccgtcat ccagatgcag cgaaaagtag tagtaagcca gcggctgatc
    12541 gttgttcttg ctatcggtaa tcgtcaggcg taaagaagcc tcttccccca cctttacttt
    12601 catcgctttt atcgtcgaat cataactgcc cggcgtcagt accagcttcg gttcaacatc
    12661 gggcgcttct ttatcgacac agctcacaat aaacgtcgta tcgtccggtt tttgcgcttc
    12721 gctgcggttc gaaacatccg ccgcatagtg ctgcccgctc atccccgcca gtgatgacca
    12781 gtagtagtta ccctgcatcg gccaaccagc cacatcctgc actgacgttg gcaccaccgt
    12841 cgccagcgca ccgaactgac gcattccggg caaaatgtcg caatgtttat ccgcaccact
    12901 ccaggtaaag gtggaccagg tttcattgtg atcgttaacc gtaccgtctt cattatcgac
    12961 ttccgccgcc agttttggtc gactaaaagt gtaatcacgg gccttcagcg tatcgggcat
    13021 atgaccccac attgtggcct gcgggacatc cgggctggtg aggacggtaa agatcactgc
    13081 cgtttcactg gtctgagcaa tacccgcgag gctcgcgacc aacggcgttt ttacaccagg
    13141 gccgttggcc tgggtgaccg taaccgtggc aacaccattc gcatcggtag taccacgata
    13201 ttccgtcgcg gtggtggtta attcggtcgt gcccagtttt accggcgcag tattgttcac
    13261 tacaccctgg cggttttcag catctttgcg tttgataaca aaaggaatat tccccacaac
    13321 attaccgacg caatctttgg tggtgaccgt taacgtgata ctgccgccaa ccgtcgtttt
    13381 cgccgcaccg gaggattcgt catagcccgc ggcgctgacg gaaataaggt caccctgcgc
    13441 gggcgcaatc ttcagcgtca ctttctgatc ggcgagggtc agaacacttt catagggata
    13501 gcccgtcgag gtaagtagat ataacagcgg catggtctgc tgggggtctg gctgcgccca
    13561 ggcgatcagc gaacgcccgg caaggccctg tgcgcaaaat gagcgatctg cgactggctg
    13621 tgggtccagt aaggtgtccg gcgtcgcggc atccgcccaa actaaagata ctgtcggtgg
    13681 cgtttggttt tcccaacgtg gtgtggcaat gatatcgcct tctctgtctt gcgggttggt
    13741 gacgagcaat ttagagacgc taacatccgc gctgaattca ctcggtttag cggtaaacgc
    13801 tacctcatgc gtctttgcag gatccagaaa gacactgccc tggtagacgg gtatgctggc
    13861 actgtcggcg gtgggcacgg taccgttaaa ttcgccgacc gtatctgaag agtcttgcca
    13921 tgcagattct gcccatgacc acccaggcag cattgcgctt atcagcaatg cgcataacac
    13981 aaggatttta ttcagggtca gggacatgac agaaactcca ccgattgtta aacggacgct
    14041 tgtcgcatca acatattttg cttaatccgg gattaagcaa aatatgcgga aacagcattc
    14101 ccttgcggga atgctgctga gtacgcgtta tttgtgtttg taaataatgt gaagtttgta
    14161 accctgtacc ccgtcgccgt ttgtcggaat ggtaagagcc tctttaccat cgcgcgcagg
    14221 accgttaagg ttggttctgg cttcgtcgtt ggtcgcaggg atggccagat tgttattatc
    14281 gaagcttgag ttaacgatac cacctgaagt gcccagctgc tggctgctgc cagaaagtac
    14341 ccaacggaag tcataattgg cggtaacgtc agcatcggta cccgcatcaa acttgccgtt
    14401 tttgttctca tccgagtaca gtttggccac gtaggttttg ccaagttcga ttggcgtgct
    14461 gccgttgata aggttgatac tggtaccatc aacaaagatg gcgactttca ggttctggtt
    14521 aaccaccggc cctggcgcaa cgttatcgct gtcgcttccg ccgccgctgg cagtggaaat
    14581 gtcataaaga tgcagcgcag tcccgacgtt tggcgtaccg gtctgcgtgg tcggcgtaat
    14641 ttcaataccg atataacgac cacggtcgtc atcggtaaga gtgtaactgg tcaccttcgt
    14701 tgccagctct ttcggatcgc ccccctgagc gtccttgtag ctcatccagc ggatagtatc
    14761 gatagtcttt gcgttatcgc ccaatccccc aagatccacg tcgccttcgg tatcaccaat
    14821 ttcccaactt acggttaatt tatcacccga gtaaagacgc ttgtccgttt cactgcctgt
    14881 cgggcgagcg ccttttagat cagaagtcaa cgttacatgg tcggcatctg tcgtagcgct
    14941 tcccccttcc gaatagagcc acggaatggt tccctggaaa atattggttt cgccgctgtc
    15001 gacccaggcg ctttcttccg ccagcgccac aggtgccaca caataacctg ccagtgccag
    15061 cgccaatgcg atccgggtca gaccgcgtct tttatcctgc ttcattgatg ctctccttaa
    15121 tgaaccttca gcgactggcc gactggcctt acgccaatct tattttttgg tatacagcac
    15181 gcgtaaaccg tatccctgca agccatctcc cgcttgtgcg ccataggttt gcgctgcttc
    15241 gcggttagtg gccggaatca caatgtcctc attggaagtg ttcgcctgtg cacccacttc
    15301 accattgatg ctcttgccgg taaattccca cttgtagtcg tactgcgcca tctcttcgtc
    15361 agtcagtttt tcgacctgct ggaagacacc gtctttattg ctgtcacgcc acaaaacaaa
    15421 tcgataagtg ttgttgagcg gcagcggttc tttatcttta cctatcaggt tgctgggttt
    15481 agccgccccc acacgataga taaatgcatt tagcccacca atttctgccc cgaggaaagt
    15541 gacatccaca tagttactgt cgtcgtaggg cgcagctttt gctttccagc gataggtgcc
    15601 gggtaaggta ctggagaggg tgacagtacc cttttcttta tcttgtttag cgataaaaac
    15661 gcaggggttg tcggtcgagc aaggcttgtt ggttaacgtc actcgtttgt cctgcgcgtc
    15721 gtaaagccgc ggcgtgaggt tatcttccgg cagcgcatcg ccgttgcggt cggtgttatc
    15781 gccaaagctg tcggttacat cgagatccat caccacacca tcaacgccat ctgcctgggc
    15841 gctggcttcg tggctgtggt tgttctcttc cggcacgttc gcgatgttat taacaatcaa
    15901 ctcaatttta cggtcctgct gaacggtgat ttccaccaca ccagagttct gtcggttgcc
    15961 cttttcatct tccagtgcca gacgtactcg ccaggtattg agtttttctt gttcggcttt
    16021 gtcagagctg tactgccagg cgggcagcac caggttccag tggttattgt taccctctgt
    16081 cagggtattc tctttgataa ccggcataac cagcgtgcgg gcagcattgt ttttcgcctg
    16141 ccatgacgca gcgacaattt tgtagtggtg gttgataagc gcgttaatgg tccacttaca
    16201 tttttccagc ccgaccgccg cttcaggcgt gtctttaatg acacactcgg ggtgttcgtt
    16261 ggtggcatcc gcttttaacc acagggtgac atcaagcgga tctttttctt tgtattccag
    16321 aacaataaag ttattacgct caacaaaatc atgacgactg cccatcaacg agtgagccgc
    16381 tttcacttta tcgctatcca attgatcttt tagcggaacg ccgaactgat aattaagagt
    16441 ggcattaatg ctgacatcgc tgttatctcc ggtccccgct ttatagtccg ccccaacggt
    16501 tagtaatggc accggttgat aattcagtcc aagtgttacc gcgtagggat ctttctccaa
    16561 attatccgta ccaaaaaggg caacttcatc gccgtaatat tgctcgaaga caactttgcc
    16621 ccccagttgc ggataagaag gtaaccaggc ttcggcgcga atatcccagc cgcgcgcagg
    16681 acgctcctca taaaaatcga aatcttcgga gtctttccag tcagaaagcg gatgataata
    16741 gttgccagag aattttaaat aatcagtcca cgcttcgccg cctaaaccta aacggcgatg
    16801 tcctcgggta aaatcataat cataaaatat atttccgccc aataaccatt catcaaaatt
    16861 atatcttacc ccaagaccaa tattcccgat tgtacgatct tcttttcgct gcgcagaaaa
    16921 ttgactgaaa taaacagtgg tttgattatc gtaccaggga acaaaataat cgatggaact
    16981 gccatccaga tcgccgccct ggccaatgga gaggttcgaa cggaccttac cgtagggaga
    17041 gagagtgtct tcaatataag attgtgcggt tgaggtgatt tgactttgca gatagcttct
    17101 ggcctgagat tccagcgcct caggggtcaa gttttcaaac ccttgcgtgg cggagttgat
    17161 gatgtaatcg gctccgcgct ctttcagcga tttacccttg ttggtttgtt catcctgctg
    17221 tgctgcctgg ctacctaaat caggtaacga ttcgttttgc ttaacattta tctctggtgc
    17281 cgcgaaagca gcaccagaga taaatgttag aataatacag cgtttccatc gcagcatgca
    17341 ctttccctgt gacaatgata cagtcaaaaa taagctttaa tattgtatcc ctgatt
//
LOCUS       DAPHCV010000102        17304 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.102, whole genome shotgun sequence.
ACCESSION   DAPHCV010000102 DAPHCV010000000
VERSION     DAPHCV010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.102"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(48..779)
                     /gene="rlmB"
                     /locus_tag="RH033_003565"
     CDS             complement(48..779)
                     /gene="rlmB"
                     /locus_tag="RH033_003565"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDV2992694.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(870..3311)
                     /gene="rnr"
                     /locus_tag="RH033_003566"
     CDS             complement(870..3311)
                     /gene="rnr"
                     /locus_tag="RH033_003566"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDV2992695.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTISSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKPKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3350..3775)
                     /gene="nsrR"
                     /locus_tag="RH033_003567"
     CDS             complement(3350..3775)
                     /gene="nsrR"
                     /locus_tag="RH033_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HDV2992696.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(3980..5278)
                     /gene="purA"
                     /locus_tag="RH033_003568"
     CDS             complement(3980..5278)
                     /gene="purA"
                     /locus_tag="RH033_003568"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDV2992697.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5382..5579)
                     /locus_tag="RH033_003569"
     CDS             complement(5382..5579)
                     /locus_tag="RH033_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HDV2992698.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5661..6665)
                     /gene="hflC"
                     /locus_tag="RH033_003570"
     CDS             complement(5661..6665)
                     /gene="hflC"
                     /locus_tag="RH033_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HDV2992699.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETMGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6668..7927)
                     /gene="hflK"
                     /locus_tag="RH033_003571"
     CDS             complement(6668..7927)
                     /gene="hflK"
                     /locus_tag="RH033_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HDV2992700.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTDPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8013..9293)
                     /gene="hflX"
                     /locus_tag="RH033_003572"
     CDS             complement(8013..9293)
                     /gene="hflX"
                     /locus_tag="RH033_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDV2992701.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9370..9678)
                     /gene="hfq"
                     /locus_tag="RH033_003573"
     CDS             complement(9370..9678)
                     /gene="hfq"
                     /locus_tag="RH033_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDV2992702.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9764..10714)
                     /gene="miaA"
                     /locus_tag="RH033_003574"
     CDS             complement(9764..10714)
                     /gene="miaA"
                     /locus_tag="RH033_003574"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDV2992703.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEIDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10707..12554)
                     /gene="mutL"
                     /locus_tag="RH033_003575"
     CDS             complement(10707..12554)
                     /gene="mutL"
                     /locus_tag="RH033_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDV2992704.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRIAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAVREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12564..13901)
                     /gene="amiB"
                     /locus_tag="RH033_003576"
     CDS             complement(12564..13901)
                     /gene="amiB"
                     /locus_tag="RH033_003576"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HDV2992705.1"
                     /translation="MMYRIRNWLVATLLLLCAPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPSPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13920..14381)
                     /gene="tsaE"
                     /locus_tag="RH033_003577"
     CDS             complement(13920..14381)
                     /gene="tsaE"
                     /locus_tag="RH033_003577"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDV2992706.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14353..15900)
                     /gene="nnr"
                     /locus_tag="RH033_003578"
     CDS             complement(14353..15900)
                     /gene="nnr"
                     /locus_tag="RH033_003578"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HDV2992707.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAIGIDVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANTH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSQWLIPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLKWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15899..17038
                     /gene="queG"
                     /locus_tag="RH033_003579"
     CDS             15899..17038
                     /gene="queG"
                     /locus_tag="RH033_003579"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDV2992708.1"
                     /translation="MSEPLDLNQLAQNIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17021..17074)
                     /locus_tag="RH033_003580"
     CDS             complement(17021..17074)
                     /locus_tag="RH033_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992709.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3931 a         4937 c         4342 g         4094 t
ORIGIN      
        1 aaaaaaaaac catccaaatc tggatggctt ttcataattc agaggaacta gctgcgctga
       61 cgaaccgctt caaataagca aattccggtt gcaaccgaaa cgttcaggga agaaacactt
      121 cctgccattg ggatgctgat caactcatcg caatgttcac gggtcaggcg acgcatacct
      181 tcaccttccg cgcccatcac cagcgccagg cgtccggtca ttttgctctg atagagtgta
      241 tgatccgcct cgcctgccgt accgacgatc cagatattct cttcctgcaa catacgcatg
      301 gtgcgcgcca gattagtcac ccgaatcagc ggaacgcttt ctgccgcacc acaggccact
      361 tttttcgccg tggcgttgag ctgtgcggag cgatctttcg gcacaatcac cgcatgaaca
      421 ccagcggcgt ccgcgctacg caggcacgcg ccgaggttgt gcggatcggt tacgccgtcg
      481 aggatcagca ggaacggttg atcgagcgaa gcgatcagat ccggcagatc gttttcctga
      541 tactgccgtc ctggcttcac acgagcgata atgccctgat gcacggcacc gtcgcttttc
      601 tcgtcgagat attggcggtt tgccaactgg ataaccacgc cctgagactc aagggcgtga
      661 atcagcggta acagacgttt atcttcacgg ccttttaaaa taaagacttc ctgaaaacgt
      721 tcaggggcgc gctccagcag ggcctgcact gcgtggatgc cgtaaatcat ttcgctcatt
      781 aatgtactcg ttatttacgt tttgccggga gcgccacgct tgcccggcct acggataatt
      841 caccctcttc tgtttgaaga gggtaatgat cactctgcca cttttttctt cgccgcacgc
      901 ttcgctttgg tcgctgcggc tattttctgc gttttcgccg atggcttttt cgcttttctc
      961 gcgtctttct tcgctgcttt cggcttcggt tttttctcgc cgcggaaggc gctgtctggc
     1021 tcaaagttga cctttttgcc gacctgacgc cgcttgccgc cttttttgcc tgcatcgcct
     1081 tttttcgctt tctcgcgcgc cgttttaccg acgttgcgcg gtgcgcgttc gctggagatc
     1141 aggctaaagt cgattttgcg ctcgtccata ttaaccgctt cgacgcgaac ttccacgcga
     1201 tcgcccaggc gataagtctg gccgctggat tcgcccatca ggcgttgccc cacctggtca
     1261 aagcgatagt agtcattgtc cagcgaagag acatggacca gaccatcaat gaacaagtca
     1321 tccaggcgaa caaagaagcc aaaaccagtg acgctggaaa ttacgccttt aaagacgtta
     1381 cctacctggt cgagcatgaa gtcacacttc agccagtcag ccacatcgcg cgttgcttcg
     1441 tcggcacgac gttccgccat cgaacagtgc tgacccagtt gcagcatctc ttccatcgaa
     1501 taatggtagc cgccggtttc agtggtattc ccctgatgcc cctgctcttt cgccaacaga
     1561 tacttaatgg cgcggtgcag cgtcaggtca ggataacgac gaatcggcga agtaaagtgc
     1621 gcataggact gcaatgccag accgaagtga ccacggtttt ccggatcgta aatcgcttgt
     1681 ttcatcgagc gcaacagcat ggtttgcagc atttctgcgt caggacggtc agcaaccgat
     1741 tccagcagtt ccgcgtagtc acgcggttcc ggcttgttac cgccaggcaa ttccagcccc
     1801 agttccgcca gcactgaacg gaaagaggtt attgcttcag tgctcggctt gtcgtgaata
     1861 cggaacagtg ccggttcttt ggccttctca acgaaacgcg ccgccgagat attcgccaga
     1921 atcatgcact cttcaattaa cttgtgtgcg tcgttacgct gggtctgttc gatacgctca
     1981 atacggcgtt cagcgttgaa aatgaacttc gcttcttcgc tctcaaacga gatcccgccg
     2041 cgttcttcac gggctttatc cagcacttta tagaggttat gcagctcttc gaggtgctta
     2101 accagcgggg cgtactgctc gcgcagatcc tgatcgccct gcagaatatg ccagactttg
     2161 gtgtaggtca gacgcgcgtg agagctcatc accgcttcat agaatttgta gcccgtcagg
     2221 cgacctttcg acgaaatggt catctcacac accatacaca ggcggtctac ctgcgggttg
     2281 agcgagcaca ggccgttaga gagcacttcc ggcagcatcg ggataacctg cgaagggaag
     2341 tacaccgacg taccacggtt acgcgcttct ctgtccagcg gtgttggcgg acgcacatag
     2401 taactgacgt cggcaattgc gacccataaa cgccagccgc cgccgcgttt tttctcacag
     2461 taaactgcat cgtcaaagtc acgggcgtct tcgccatcaa tggtgaccag cggtaaatcg
     2521 cgcagatcga cacggcccgc ttttgcttct tccggcactt cttctttcag cccggcaacc
     2581 tgttgctcaa cagcctgcgg ccagatgtac ggaatttcat gggtacgcag agcaatatca
     2641 accgccatgc cggtgcccat attatcgccc agcacttcga cgattttacc caccgctttg
     2701 gtacggcgag tcgggcgttg agtcagttca accaccacca caaagcccat ccgcgcgccc
     2761 atgatctgat cgggagggat taagatatcg aagctcagac ggctatcgtc aggaaccaca
     2821 aagccgacgc ccgcttcggt gaagtagcga ccaacaatct ggctggtttt tggcaccagt
     2881 acgcggacaa tacgcgcttc acgacgacct ttacggtcag cgcccagcgg ctgagccagc
     2941 acctgatcgc catgaatgca ggttttcatc tgctcgctgg agagatacaa atcatcttta
     3001 cgcccttcaa ctcgcagaaa gccgtagcca tcacggtggc caataacggt acctttcacc
     3061 aggtcgaggc gttccggcag cgcatagcac tgacggcgag tgaagaccag ttgaccatcg
     3121 cgctccatcg cgcgcaggcg gcgacgcagg ccttcaagct gctcttcgcc ttcaatgtgc
     3181 agttctaccg ccagctcatc acggctggcc ggtttttcac gtttggttaa atgttcgagg
     3241 ataaattccc gactagggat gggattcgcg tatttttcag cttcgcgttc ctggaaagga
     3301 tcttgtgaca tctcggttcc tccgttgtca tctctgatga agattttcgt cactccacca
     3361 gcaataattt ataaagcggt tgattctctt caaccaaatc ggcaagcgtg tagttatcca
     3421 gttccgtaag aaaactttgc acggccttag aaagcgcctg tttcaaccga caggcaggtg
     3481 taatgtggca aaactcactg ctgcaattca ccagcgataa gggctccagc tcgcgcacca
     3541 catcaccaat acgtattgca ctcgccggtt tacccaagcg aatgccgcca ttttttccac
     3601 gaacagcagt cacgtagccg gcacgactaa gttgattgat tattttgacc atatgattac
     3661 gggagacgcc atagacgtca gtcacttcag aaatactggt catccgccct tctggcaatg
     3721 acgccatgta gatcagcgca cgtaatccgt aatcagtgaa actcgttaac tgcacatcaa
     3781 cctcaaaagg gaaatcgggg aaaaatacat ttatattgat gataaaccag ccacaagctg
     3841 tgtcgctaat ttatttcagt tcggggaagg aaaaaagcga ggatttaaca ctgtgtcgga
     3901 tagcgtaatg gcttaaccga cacagcaatg tatcacactg ttagcccggc aggcaaaata
     3961 tctgccaggc gtaccagaat tacgcgtcga acgggtcgcg cagaatcatg gtttcagtac
     4021 gatccggacc agtagagatg atatcgatcg gcacaccggt cagctcttca atgcgcttga
     4081 tgtagttcag tgccgcctgc ggcaggccgc tacgatcttt cacgccgaag gtggattcag
     4141 accagcccgg catggtttcg taaatcggct ctacaccttt ccagtcgtca gctgccagcg
     4201 gagtggtagt cacttcacga ccatccggca tacggtaagc cacgcagagt ttaacctctt
     4261 tcaggccatc cagaacgtcc agtttagtca ggcagaagcc agacagagag ttcagctgta
     4321 ccgcacgacg aacggcaacg gtgtccagcc agccggtacg acgacgacga cccgtagttg
     4381 cgccgaattc gttaccctgc ttgcagagga actcgccagt ttcatcaaac agttcggtcg
     4441 ggaacggacc tgcacccaca cgagtggagt aagctttgag gatacccaga acgtaatcaa
     4501 cataacgcgg gcctaggccg gaaccggtcg ccacgccacc agcagtggtg ttggaagaag
     4561 ttacgtacgg atatgtaccg tggtcgatat ccagcagcgt accctgcgca ccttcaaaca
     4621 tgacgaaatc gccacgctga cgcgcctggt caagcaggtc agaaacgtca acaaccatag
     4681 aagtcaggat gtcggcaaca gccatcgtat catccagaac tttctggtaa tcaaccgctt
     4741 cagctttgta gtagttaacc aactggaagt tgtgatattc catcacttct ttcagttttt
     4801 cagcgaaggt ttctttgtcg aaaaggtcgc caacacgcag accgcgacgt gccactttat
     4861 cttcataagc aggcccgata cctcgaccgg tggtgccgat cgctttcgcg ccacgcgctt
     4921 tctcacgcgc gttatccagc gcaacgtgat aatcaaggat tagcggacat gcttcagaca
     4981 gcagcagacg ctcacgaacg gggatgccac ggtcttccag ttctttcatc tctttcatca
     5041 gcgcggccgg agacagcaca acaccgttac cgatgatgct ggttacattc tcgcggagaa
     5101 tacctgatgg aataagatgg agaacggttt tttcaccgtt gattacgaga gtatggcctg
     5161 cgttgtgacc gccctggtag cgtacaacat atttagcccg ttcagtcaga agatcgacga
     5221 tcttaccttt accttcgtca ccccattggg tgcccagtac gacgacgttg ttacccattt
     5281 ttcaaaatca ccgtttgctt aaaaatggat tctaccatcg ctttttcaga gttacagcac
     5341 tttttgcact caaaaatgac ctcgatcagt ctgttttttg ttcagccaat cgttttcctc
     5401 aacatgtagt agaccacaac gcccgcaacc acaagtccac cgccaaaacg acgtaaaata
     5461 ttatcgggca aattggtcat cgcagagatc atcttcttcc atgccttcgg gtaaagcatc
     5521 ggccctaaac cttccagtac caaaaccagg gcaagcgcca gccagattgt cgaattcatt
     5581 cattatcctt atagaaaaag aaaaccaccg acatccctga ggatgcggtg gctttattga
     5641 cctgtaccgc agtcgttata ttaacgcgtt gcggaagtcg gcgtcttcat gtagcggaag
     5701 aaatcgctat ccgggctcat gaccatcacg tcctgattgc cagagaagct gttctcataa
     5761 gcacgcaggc tacggataaa tgcgtagaag tccggatctt tactgaatgc atcagcaaac
     5821 agtttggctg cttctgcatc gccttcacca cgcatgatgc ggccctgacg ctcagcttca
     5881 gccagcgttc tggtcacttc atagtcggca gtcgcgcgca gtttttccgc ttcttcctga
     5941 ccttgtgaac ggtgacgacg cgctaccgct tcacgctcgg cgcgcatacg gttgtagatc
     6001 gcttcagaca cttcggtcgg caggttgatc tgcttaatac gcacatcgac aacttcaata
     6061 cccagcgccg ccatactgtt cgggttgatg accggaactt tgcccatcgt ctcagccgtt
     6121 acgcgctctg ccgcttcggc aatggcgtta tctgctgccg gggtagtaac ttcatcttct
     6181 gtacccgcag aaccggagtt cagcgcgtca cgtacttcga gggtcagacg accacgggaa
     6241 tcggtgacga tgtctttcac gtccaggcga ccaatttcag aacgcagacg gtcagagaac
     6301 ttacgtttca acagcacttc cgcttgcgaa atgtcgccac cgcccgttgc caggtagtaa
     6361 cggctgaaat cgctgatgcg ccatttgatg taagagtcga cgatcaggtc tttcttctct
     6421 ttggtcacaa agcggtcggc ctggttgtcc atggtctgaa tacgtgcgtc gagcattttc
     6481 accgtttcaa tgaacggtat cttgaaatgc agacccggct cataaaccag aggtttgttg
     6541 tcatcgtcac gcagtacctt accaaaacgc agcgtaatac cgcgctcacc ttctttgacg
     6601 acaaagacag acatgtaaag cactaccagc acgatgatga taatcgcgat aactgactta
     6661 cgcatcgtta ttccccctga cgctggtagt cgttacgctg cgcgttggcg cggcgttggt
     6721 ccataatatc gccctgactg gtggacgacg tgttgcttgc tccactggtt gtggaggaag
     6781 aggctggcgg cagacgcagc agattgctgg caccgttatc gctcttcgcc gcaggggcgt
     6841 taccaccttt cagcatctgg tctaacggca gaaccatcaa gttgccacct ttatcgttaa
     6901 ccagcacttt gcgggtgttg cccaacactt tttccatcgt ctcgatatac agacgctcgc
     6961 gagtaatttc cggcgcggct ttatattccg gcagaagttt agcaaagcgc gccacttcac
     7021 cctgagcttc caggatggtc tgggccttgt acgcacgcgc ctcttcgagg atacgttgcg
     7081 cctgaccgtt cgcacgcggc tgaacttcgt tggtatacgc ttctgcttca cgaatgtatt
     7141 gctgttcgtt ttcacgcgcg gcaatcgcat cgtcaaacgc cgcttttact tcttccggcg
     7201 gacgagctgc ctggaagttg acgtccagca gcgtgatacc catgtcatac ggacgaatcg
     7261 tctcttccag ttcgcgctga gtatcgctac gaatcacggt acgaccttcc gtcagaatgc
     7321 ggtccatggt gtatttaccg ataaccccac gcagggcgct gtcggtagcc tgacgcaggc
     7381 tgtcatccgg gctggtcacg ctatacagat atttttccgg atcggtgacg cggtactgca
     7441 cgttcatctc aacgcgcact acgttctcgt ccgacgtcag catcacacca gaagcggcca
     7501 gttcacgcac ggcttccacg ttcaccggtt tgacttcgtc gataaacgtc ggtttccagt
     7561 tcagacccgg ctcaaccaga tggctgaatt taccaaagcg tgttaccacg ccacgttcgg
     7621 cttctttaat ggtatagaaa ccactggccg cccagataat gacaatcgct gccgctgcga
     7681 tggtaacgac acgaccgcca agctgcgggc gcgggccttg cgatgaactg ccaccgccag
     7741 atccggtgcc tttaccgccg cccagaccac cgagcttttt gctcagtttg cggaagatat
     7801 catctaaatc aggtggccct tgatcgcgac cgcctttgtt tccatttccc tcagagttgc
     7861 cgccaggttt gctgcttccc cacgggtcgc ggtcttgtcc gttattaccg ggctgattcc
     7921 acgccatgtt tgtgctccat atttgttatg cggtgatccc tgttgtcttt cgacgccaga
     7981 ggatatgact ccacgcttca gacgctacgc cgttagatca ggtaatcgat caacgccggt
     8041 tcttgtttac agaggcgacg ccagtcaacg atcggcatac gaacttgcag acttacgctg
     8101 ccgtcctcct ccatccactc tttttctatt gcctgaagct gataaaaacg acttctcaga
     8161 cgcccttcct gcggtggcag acgcaatgta tgctgcgcca cctcgccgga aagccgctcc
     8221 gtcaaagcct gaaaaagctg tggtatcccc gctccggtct gtgcggaaag ccagacacgg
     8281 atcggtttgt tctcttcgtc ccgatcaata cgcggttcga aatcttccag catatcgatc
     8341 ttgttcatca ccagcagggt tgggatctcg tgagcgtcga tctcttcaag aaccgtattc
     8401 accgcttcga tgttttcttg tacacgcaca tccgccgcat caatgacgtg cagcagcaat
     8461 gtggcttgcc gcgtctcttg taacgtggct ttaaatgccg ccaccagatc gtgcggcagg
     8521 tggcgaataa accctacggt atctgccaac acggtttcac cgacatctgc gacgtcaata
     8581 cgccgcaacg tcgggtcgag ggtggcaaac aactggtctg ccgcgtagac ccgcgcttcg
     8641 gtgatgcgat tgaaaagggt agatttaccg gcgttggtat atcccaccag cgaaacagta
     8701 ggaacgtcgg ctttgatacg cgattgccgc ccctgctcac gctgcttttc aactctttcc
     8761 aggcgcgact gtatctgcac gatgcgatta cgcaacaaac gacggtcggt ttcgagctgg
     8821 gtttcacccg gaccacgcaa accaatcccg cctttctgtc tttcaaggtg ggtccagcca
     8881 cgcaccaggc gcgtagccag atggcgcagc tgcgccagct caacctgcaa cttaccctca
     8941 tgggtacgcg cacgttgggc gaaaatatct aaaataaggc cggtgcggtc gatgacacga
     9001 cactcgcaca aacgctccag gttacgctct tgcgccgggc tcagggcatg gtcaaaaaga
     9061 acgaccgaag cacccgtcgc tttgacagct tccgcaattt caactgcttt accttcacct
     9121 acaaaatact ttgggtgcgg cgctttacgg ctaccggtaa tcacctgcaa tgcttcgaca
     9181 ccggcggaag agaccagaga ttcaaactcc tggagatctt ccatatcttt gtcttgcgta
     9241 aaatagatgt gtaccagtac cgcctgctca ccagcatcat aacggtcaaa caagcgtata
     9301 accctctaaa tagatcagcg gggaacgcag gatcgctggc tccccgtgta aaaaaaacag
     9361 cccgaaacct tattcggttt cttcgctgtc ctgttgcgcg gaagtattct gcgcgctgct
     9421 accatgatgg tagttactgc tggtaccgcc accggcgttg ttactgtgat gagaaaccgg
     9481 gcgagacggg acaacagtag aaatcgcgtg cttgtaaacc atctggctga ccgtgttttt
     9541 caacaggatc acgaactgat caaaagactc gatttgccct tgcagcttaa taccattcac
     9601 caaataaata gaaactggaa cacgttcccg acgcagtgcg ttcaggaacg gatcttgtaa
     9661 agattgcccc ttagccattc tctcttttcc ttatatgctt atttgtactt tgaacctttc
     9721 gattctgaaa aaattgcgca cgatacgtct caattgtaca cattcagcct gcgatagcac
     9781 caacaacctg taatacttcg tcacgcgctt gttctggttt ttcactgtca agccagtgaa
     9841 ccccttccca accacgcagc caggttatct gccgcttcgc caactgtctc gtggcgcaaa
     9901 cacctcgata aaccatttca tcgtatgaga tttcgccttc aaggtaagac cacatctggc
     9961 gataacccac gcaacgaatg gaaggcaagt ccgtatgcaa atctcctcgg gcaaaaagcg
    10021 cccggacttc tgcttcaaaa cctgaagcca acatctgatg aaaacgctgc tcaattcgtt
    10081 gatggagcag ttcacggctc gccggggcga tggcgaactg atgcacctga tacggtagag
    10141 cgtctcctga cgtttgcgtc agttccgtta aagttttacc cgaaatgaaa aaaacttcca
    10201 gtgcccggga aagcctttgt ggatcatttg gatgaatcct tgctgccgca accggatcga
    10261 tctcctgaag ttgacgatgc aatgactccc aaccttgctc tgccgcctgt tgctcaattc
    10321 tggcccgcac ttccgggtcc gccgacggta gcggcgacaa cccttccagc aatgccttga
    10381 aatacaacat cgtaccgccc actaacagtg ggatccgccc cgccgcggtg atatcggcca
    10441 tttccgccag cgcatcgcgg cgaaaatcag cagccgagta agcctgcgac ggatcgcgaa
    10501 tatccagcaa tcggtgcggc gcggcgagta actcttcagc gttcggcttc gccgtcccga
    10561 tatccatccc tttgtaaata agggcagaat caacgcttat caactctact ggtaaaattt
    10621 tacgcagctc aatggctaac gccgttttac cggaggccgt cggccccatc aaaaaaatcg
    10681 ccttaggcag gctcgcctta ctgatatcac tcatctttca gggcttttat cgccggatgt
    10741 aaatcaacag attgtaacag accacccggc ggcgttttca caagttgcgg acataaccgt
    10801 tccacgtccg ccagcagggt tatggcctgc gccattgacc actgcgcatg ttcgctcatc
    10861 agatttcgtg caatccactg cgcaatattg ccaggttcga atacggactg cttcgccagg
    10921 tagcctatca gttcaggaat caagatttgt aaattttgtt ggcgtaaggg taaaggcacg
    10981 gccctgatgg tcacatgctg cgcatcggac tggaaatcaa tacccaattc cgccagggca
    11041 gactgcgctt tttctaatgc cgatttttct tcaccagaaa cttttagccg cagcggaatc
    11101 agcaacggct gggcgcaaac tggcgcttca cccggcgtca gttgcgcctg acgcagccaa
    11161 cgttctgcca ctggcaaggc taaaagtgaa atgttgccgt cgcgctccag caacgcacag
    11221 tcggaatgga cgatagtcag tacccgacca aaactctgac tgttcgccgc aagtgcaggt
    11281 tcctgcggtt ctggcgcttt tggtttttgc atcggcgcgg gcgtttgcaa aagctggcga
    11341 tacacttcac cttgctgttt ctggtaacct ggctgcgcat tcggccaagg ggcagccgga
    11401 cgactgcctg aggcgggcgc aggagtgtaa cgcggagcta ctggctcacg aactgccggt
    11461 tcagcaaaat gattgcgccc cgccgccacg cggttttccg ggatggcgcg cggtgcaggt
    11521 tgtggttcat cgtccagcgg tagcggcgtt tccagttgct gttgtagcac gctcagcacg
    11581 ccctgataga taaaatcatg caccagacgc gactgatgga aacgcacttc gtgtttggca
    11641 gggtgcacgt tgacgtccac ctgatgtggg tcgatctcca gatacagcac aaatgccggt
    11701 tgctgatcgg cccccagttt gtcttcacag gcctggcgga tcgcgtgatt gatcaggcga
    11761 tcgcgcatca tgcgaccatt cacatagcaa tactgaattt ccgccagtgc gggcgtggtg
    11821 tgattcggat ctgccaccca gccgcgcagc gtaagatcgc cgtgttgcca ttcaatcgcc
    11881 agcgcttgtt cgagaaaagc ggtgccgcaa atcgcgccta agcgccgttc tttttgcccg
    11941 ccttccggca ctgcgcggta ctgacgcaca attttaccgt tatgcgacag gttgatcgtg
    12001 acgtcgaaac gcgccagcgc aatgcggcga ataatttcat cgatatggtt aaattcggtt
    12061 ttctcggtgc gcaggaattt gcgccgcgcc ggggtgttgt agaacagatc cagcacctcc
    12121 agcgtcgttc ccaccggatg cgccgccggt ttaaccgtta cgtccatatc gcgcccttcg
    12181 gcataggcct gccaggcttc ctgctgttct gcgatgcgtg aagtgagcgt caggcgcgaa
    12241 accgaactga tactcgccag cgcctcaccg cgaaagccaa ggctgataat ggcttcgaga
    12301 tcgtccagag aggcgatttt actggtggca tgacgagcca gcgccagcgc cagctcatct
    12361 tttttgatac cgcagccgtt atcacgaatg cggataagtt tcgccccacc gcgttcgata
    12421 tcaatatcga tacgcgtcgc acctgcatcg aggctgtttt ccaccagttc tttgactacc
    12481 gacgcaggtc gctcgaccac ctcacctgcg gcaatctggt tcgccagttg tggcggtaag
    12541 acctgaattg gcatcaatcg tccttagttt ggcagcgtgc gatctggcgt cgtcaccgta
    12601 ctggcagttt gtgccgttgc accctgcggc gcagattgca tcggatgcgc aaggaaataa
    12661 ttacgcaggc ctttgtaaat ggcttctgcc agctgttgtt ggtaatcgtc gctcgccagc
    12721 aaacgttctt cgctgttgtt gctgataaaa ccggtttcga ccagtactga tgggatatcc
    12781 ggcgaacgca gaacgccaag gctggcgtgt tctggtcgac gtttatgtat ttcgccaatg
    12841 cgttgcaact gactgatcat actggtcgct acatcatacc ctacacgctg ggaatgaccg
    12901 aactgtaaat ccagcaccgc ctggcttaaa taggggtcag actggctgtt cgccagcaca
    12961 tcgcccgctc cgcccaatag ctccgactgt ttctcatgct gttccagcca gcttgccatc
    13021 tcgctgtttg cacgacggtt agagagcacc catacggaag cgccagtcgc actgcggttt
    13081 ggtgcggcat cagcgtgaat cgacacgagg aaattggcgt tttgcttacg tgccacatcg
    13141 ctgcgcccca tcaccgagat aaagtaatcc ccgtcacggg ttaaaacgcc tttaaacatt
    13201 ggatcgtcat tgagtaaagt acgcaattta cgcgcgatgg cgatggtgac atttttctcc
    13261 cgcgtgccac cagggccgat agcgccagga tcctgaccgc cgtgtccggc atcaatagcg
    13321 atgataattt tatcgccagt gttagccgtc gcgcgcgctg ccggacgcgt taccgtatta
    13381 ctgctgataa cacctgtagt gcggttactt tccgttttaa acggattgcg cgccggttcg
    13441 ctgacgcgcg gtgcgacaac cgcaggcgtt tcaacgcgtt tagcaaccac aggcggcgaa
    13501 ggaggcggtg gcggcacatc ggcgttaatc gtaaagacga cagtgtaatt gctgccattc
    13561 tgccgcttca ccgcttcggt tttaccgttt tcggttagat cgaccaccag ccgtagcgtt
    13621 tgtgcatctt taggcgttcc ggagcgaatc gccttcacca gattattgcc gctaaacaac
    13681 aacggcagtc cctgaatcac gcccgtttgt ttgatatcga gcgccacggt gcgtttgctt
    13741 tgatggctaa acgcataatc aggatcgcca ataaaactca acgttatccg cgcctgctga
    13801 ttgccgttag aaacctgaat atcagagagc gtcgcggcac ccaccggcgc gcacagcagc
    13861 agcaacgtcg ctaccaacca atttctgatg cgatacatca tcccgccacc tttcaaaggt
    13921 taaccggcta aacgcgccag caacaattca cccgcagagg aaaccgcact cacgcgcgcc
    13981 tcacggcctt gtgcctgata atcaatgtgt atttcgacat ccgggtcagg aagaacacct
    14041 gtaccttgtt gtggccactc caccaggcag atggcatcgt tggcaaaata atcgcggatc
    14101 cccataaact ccagctcctc gggatcggca aggcggtaca aatcaaagtg ataaaccatt
    14161 aaattgtcga gcgtataggg ttcgaccagc gtataagtgg ggcttttgac attaccctga
    14221 tggcccaaag cctgcaaaaa gccccggcta aaggtggttt tacctgcgcc taaatcgcca
    14281 tacagataga ttacggttgc gccatcgcag gctttcgcta cccgctcgcc caggtctaat
    14341 gttgcctgct catcagggag cggaattact cgattcatca tggtttttat cagtcacttc
    14401 cgggttaaca atacgctgta gcgtggaaaa gagatcggtt gccagcatcc cgcgcgttcc
    14461 aaaacgcgcc gccagtacgt cagctgccgc accgtgcgcg acacagcccg cacaggctgc
    14521 atcatacggc gacagttttt gcccaagcaa tgcgccaata ataccagaga gcacatcgcc
    14581 catgccgccg ctcgccatgc ctgcattccc ggcatcaata atgcctaaag cgtcaggatg
    14641 ggcggcgacc acggttccgg cacctttcag caccgctacg ccgccataac gttgtaccag
    14701 acgtttggcg caatgtaagc ggtcactttc aatttcagcg acggaacagc ctaacaaccg
    14761 tgcggcctcg ccaggatgtg gcgtgatcac gcgattgtga cgcttatcgg gattgattgc
    14821 cagcaggttc aatgcatcgg catcccacaa catcggtttg cgaaaattct caactttttg
    14881 cagggctttt ttcccccact cttgctggcc cagaccggga ccaatcacca ccacatcggc
    14941 ccatttcagg ctttcggtaa gagagtccat agtcagttca tgcaccatca attctggtcg
    15001 tgcagtcagc agcggcgcaa tgttctcact gcgggtcagt actcggacta aaccagcacc
    15061 agcacgtagc gccgcttccc ccgtcatacg aatagccccc gccgtgccgt gatcgccacc
    15121 gataatcacc agccgcccgt gatcgccttt atgcgaagtc gggcgacgcg gtatcagcca
    15181 ctgagaaagt tgttctgccg aaaaccgctg aattttcgtc tcctgacctg ccagccagct
    15241 atccagcccc agtgagtcaa aatgcagttg tccggtaaca tcccgcgctt ttccagtgag
    15301 caagcctggt ttcagcgcaa taaaagtgat ggtgtgatca gcgttgatta ccgcgcctgg
    15361 cgtagcgccg gtttcagcca gcaggccgga agggatatca accgccacaa tcggcgcagg
    15421 gtgagtatta gcgtggtcga ttaactggct aatggattcg cggggcgctt gctgcaagcc
    15481 ggtgccgagc agcgcatcaa caatcagatc taccgattcg ggccagacaa tattcgaagc
    15541 atggatctcg cctcccgcgt ttaaccatgc ttcgcgtgcc agcgcggcct cttccggcaa
    15601 cggtttgtca ctttcctggg ccagcagcgt gacatcaata ccgatcgctt tggccagccg
    15661 cgcgaccacg tagccatcgc cgccgttatt accatgaccg cacagcacaa gccagtggcg
    15721 ggcgtcagga taagccgaac gacacacctg gaatgcggct tcgccagcgc gaagcatcag
    15781 ctcatagagt gtgagcccca gcgcatctgc cgcctcgcgt tctccgcggc ggatatcgtc
    15841 ggcgtaccag acggtgtgtg gtatacttac ggggtttttc ttcattgtat ggtccgtcat
    15901 gtcagagccc ctcgatctca atcagttagc gcaaaatatt aaacagtggg ggctggaact
    15961 gggctttcag caggtaggta ttaccgatac cgatctcagc gagtccgagc ccaaactgca
    16021 agcatggctg gacaaacaat accacggcga aatggactgg atggcgcgtc acggtatgct
    16081 gcgcgctcgc ccccatgagt tattgcccgg tacgctgcgc gtgatcagcg tgcggatgaa
    16141 ttaccttcct gctaacgccg catttgccag cacgctgaaa aaccctaagc tcggctatgt
    16201 gagccgttat gcgctgggcc gtgactacca caaacttctg cgcaaccgac tcaaaaagct
    16261 gggcgagatg attcagcaac attgtgtttc gctgaatttt agaccgtttg tcgattctgc
    16321 gcctattctc gagcgcccgt tagctgaaaa agcaggactt ggctggacag gtaagcactc
    16381 acttatcctc aatcgcgagg ccggttcgtt cttcttttta ggcgaattgc tggtcgatat
    16441 tcctctgccc gtggatcaac cagtcgagga aggatgcggt aaatgcgtgg cctgtatgac
    16501 gatttgcccg accggagcca tcgtcgagcc atataccgtc gatgctcgcc gctgtatctc
    16561 ttatctcacc atcgaactgg agggggcgat cccggaagag ttgcgaccgt taatgggaaa
    16621 tcgtatttat ggttgcgatg actgccagct tatctgcccg tggaatcgct attcgcaact
    16681 cactacagaa gacgatttca gcccgcgtaa gccgctacac gcaccggaac tcattgagtt
    16741 attcgcctgg agtgaagaga agtttttaaa agtcacggaa ggttcggcga ttcgccgtat
    16801 aggtcacctg cgttggctgc gtaatattgc cgtagcctta ggcaatgcgc cctgggatga
    16861 aacgattctg gcggcgctgg agagtcgtaa aggtgagcac ccacttctcg atgagcacat
    16921 agcgtgggcg attgcgcagc aaatcgagag gcgaaatgcg tgcatagtcg aagtgcaatt
    16981 accgaaaaaa cagcgtctgg ttcgggtgat tgaaaaaggg ttaccgcgtg acgcctgatt
    17041 catccacagc ttgtgaataa aaataaaaac gcattgcaat tcaagacgaa taaaatcgtc
    17101 aagtgatcga ctcgacaaaa cgaaatctta atttttaact tatctatcaa taggttaaaa
    17161 aaatactatt caccgtgcga agtttctttg aaattaagat tacggcctga ggctgtggat
    17221 aactctgttt acaagagttt gtcagagaca ataaaaacca gcaataacgc atcgcgtcgc
    17281 tgtggatatt ttattgagag aaga
//
LOCUS       DAPHCV010000103        16640 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.103, whole genome shotgun sequence.
ACCESSION   DAPHCV010000103 DAPHCV010000000
VERSION     DAPHCV010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.103"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(66..218)
                     /locus_tag="RH033_003581"
     CDS             complement(66..218)
                     /locus_tag="RH033_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HDV2992710.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(482..1216)
                     /gene="cysH"
                     /locus_tag="RH033_003582"
     CDS             complement(482..1216)
                     /gene="cysH"
                     /locus_tag="RH033_003582"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDV2992711.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNEINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(1290..3002)
                     /gene="cysI"
                     /locus_tag="RH033_003583"
     CDS             complement(1290..3002)
                     /gene="cysI"
                     /locus_tag="RH033_003583"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HDV2992712.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKVAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3002..4801)
                     /gene="cysJ"
                     /locus_tag="RH033_003584"
     CDS             complement(3002..4801)
                     /gene="cysJ"
                     /locus_tag="RH033_003584"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HDV2992713.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAAIEWRARVVDALKSRAPVTAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVDGKTLTLSEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKIYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5120..5482
                     /gene="queD"
                     /locus_tag="RH033_003585"
     CDS             5120..5482
                     /gene="queD"
                     /locus_tag="RH033_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HDV2992714.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEILAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5560..6831
                     /locus_tag="RH033_003586"
     CDS             5560..6831
                     /locus_tag="RH033_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDV2992715.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLVERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLLGDALRSCVNTGISVRGMDMALTGTQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6822..7082
                     /locus_tag="RH033_003587"
     CDS             6822..7082
                     /locus_tag="RH033_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HDV2992716.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQTVQRLINACPAGLFSLTPE
                     GDLRVDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7099..7674
                     /locus_tag="RH033_003588"
     CDS             7099..7674
                     /locus_tag="RH033_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HDV2992717.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     QIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7821..8681)
                     /locus_tag="RH033_003589"
     CDS             complement(7821..8681)
                     /locus_tag="RH033_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDV2992718.1"
                     /translation="MNITIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGALKNATLPSGMQQLNIVPGAPPDWLISVENLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLVEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKAVLEA
                     LVTTIHADCQ"
     gene            complement(8678..9457)
                     /locus_tag="RH033_003590"
     CDS             complement(8678..9457)
                     /locus_tag="RH033_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDV2992719.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTSMSLTALSMGDERALHWLRYLMALGFEEAVVLETAADLRFASE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDAPFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAELPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQNYLRQRMQP"
     gene            complement(9435..10844)
                     /locus_tag="RH033_003591"
     CDS             complement(9435..10844)
                     /locus_tag="RH033_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2992720.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFIGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGVLLGAFSVVWTVGYVLASIAGHHFISESPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVATATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLAAVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10866..12320)
                     /locus_tag="RH033_003592"
     CDS             complement(10866..12320)
                     /locus_tag="RH033_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDV2992721.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYVLEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNHLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12390..13175)
                     /locus_tag="RH033_003593"
     CDS             complement(12390..13175)
                     /locus_tag="RH033_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV2992722.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASQASNYVNGHLLVVDGGYLVR"
     gene            13494..14771
                     /locus_tag="RH033_003594"
     CDS             13494..14771
                     /locus_tag="RH033_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2992723.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSTNNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGYWQDTLPAAEAYRNMWL
                     MGMAALAMVIIFTFLLFQKIRTADSAPAMASSK"
     gene            14798..16276
                     /locus_tag="RH033_003595"
     CDS             14798..16276
                     /locus_tag="RH033_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDV2992724.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFASHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKTWAWSED
                     TAVMEKFNIPRQMLFDVQMPGTVLGHITRQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDAKAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFMPVESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         3937 a         4383 c         4212 g         4108 t
ORIGIN      
        1 aaaaaagggt gcaggagacc aaaccatcac aattcagtac gcggggtttt acccccgcgt
       61 ggtggctact tcttcgattc gtaagcgaga actgccttaa actccatacc acgttctctg
      121 atactcaact cacacaacga gtcgcctacc atcagcgtga atcccagtac tgtacaacac
      181 agtacgatga ttgccacaag ggcatatttt gtcagcatgt cttgcctccc tgcaaagaag
      241 agactaacat tcgaattgct taggttcgaa cgctggcctc aggttgatag aaatatcgcc
      301 tggggctttt gtccatctgg aacctcgcga atgcttaacg ccagacagcc tcaagcaccc
      361 aactccattc tatacctgtt aattctccct gcgatgttca cctttaaatg ccggaatgct
      421 gctcgcaaaa taaaaacgcc ccatcatttc tgacaggggc gtttaattta tccggcaata
      481 tttacccttc gtgtaaccca cattctcttt ttaagccaaa gaaacgcgtt tcttcttccg
      541 ccatgccggg ttcccattta cgggttgtat gggtatcacc gaccgataaa tatccttcgt
      601 cccataatgg gtgatatttc aggccatgtt tttgcaggta ctgataaata gttcggttat
      661 cccagtcgat aatcggcagc actttaaata cgccacgctg aattgccagc accggtaaat
      721 tggcgcggct gccggattgt tcacggcgca ggccagcaaa ccaggtttgc gcattcagtt
      781 ctttcagcgc ccggttcatc ggttcgactt tgttgatctc gttgtacttc tcaatcccct
      841 caacaccctg ctcccacagt ttaccgtagc gcgcttcctg ccaggcggcg ctttcggtag
      901 cgcggtacac tttcaggttg agcttgagtt tgtccgttaa ctcgtcaata aagcggtagg
      961 tttctgggaa cagatagccg gtatcggtga ggatcaccgg aatatccggg cgaatctgat
     1021 tcaccagatg caggctcacc gccgcctgaa tgccaaagct ggaagaaagt acatattcac
     1081 cgggcagatt atccagcgcc caggcaacgc ggccttcagc gtccagtttt tccagttcgg
     1141 cgttagtttc cgccagcgcc agaatgcgat ctaccttcgg cagttcgttc agggcgttta
     1201 gatcgagttt ggacatagat tcctcactgt ttgccttgcc tgatgcgacg ctcacgcgtc
     1261 ttatcaggcc tacaagaccg ggctaatggt taatcccaca aatcgcgcgc cggatcgagc
     1321 accgggcgaa tgatgcccgc acgcaccgta aagtcgccga agccttcacc cgcttcgcgc
     1381 tctttcgccc agcgccctat cagttcatca agcgacgcca ggatttccgg ctcggtgatg
     1441 ttttctttat acatccgtgg gatacgtgtc ccaatgcggt tgccgccaag atgcaggttg
     1501 tagcgacccg gcgctttccc caccagaccc acttccgcca gcatcgcgcg accacaaccg
     1561 ttcgggcagc ctgttacacg catcacgata tgctcatcgc tgacaccatg tttcgccatt
     1621 aaattatcga tgttgtcgat aaaagacggt aggaaacgct ccgcttccgc catcgccagc
     1681 gggcaagtcg ggaatgacac gcaggccatt gagttttcac gctgcggcgt gacggcattc
     1741 attaacccgc tttctttggc gaccttctcg atcttcgctt tctcgctttc cggcacgccg
     1801 gcgatgatca gattctggtt cgccgtaatg cggaaatcac ctttgtggat cttcgcgatc
     1861 tccagcaagc cggttttcag cggacgtccc ggataatcaa gaatgcgacc attttcgata
     1921 aacagcgtca ggtgccagtt atcatcaatg cccttaaccc agccaatacg atcgccgcgt
     1981 ccggtaaact catacgggcg aatcggctcg aatttgatcc ccgcgcgacg ttcaacttcc
     2041 gctttaaacg tctcaacccc tacgcgctcc agcgtgtatt tggttttggc atttttacga
     2101 tcggttcggt taccccagtc acgctgagtt gtcacgacgg cttccgccac tgccaacgta
     2161 tgctccagcg gcagatagcc aaactcactc gccgtgcggg cgtaggtttt cttgttgcca
     2221 tgttcgatgg aaagcccacc gcccaccagc aggttaaagc ccaccagctt gccgttttcg
     2281 gcgattgcca cgaagttcat gtcgttggcg tgcagatcga tatcgttctg cggcgggatc
     2341 actaccgtgg ttttgaactt acgcggcagg taggtctgac caaggatcgg ctcttcatca
     2401 gtagtggcga ctttttcctg atcgagccag atctccgcat acgcgcgggt acgaggcagc
     2461 agatgttcag agattttctt cgcccattca tatgcttccg cgtgcagttg cgactcgtaa
     2521 gggttcgagg tgcagagtac gttacggttc atgtcgttag cagtcgccag cgcatcaagg
     2581 ccgaccgagt gcagcatctg gtgcaccggt ttgacgttct ttttcagaat gccgtggaac
     2641 tgaaacgtct ggcggttggt caggcgaatg ctgccataga tggtgttttc accggcaaat
     2701 ttgtctatcg cctgccactg tttggtggtg atcacgccac ccggcagacg gcagcgcaac
     2761 agcatcgcgt ggcgcggctc cagcttctgt tcagcacgtt cggcgcggat gtcgcggtca
     2821 tcctgctgat acatgccgtg gaagcggatc agcaggaagt tgtcgccctt aaagccgccg
     2881 gtcagaccgt cgtttaaatc ttccgcaatg gtgccgcgca ggtagttgct ttcaagcttc
     2941 atgcgctcgg cgtctgtcag ttttccttcg accactaaag gccctggatg tttttcgctc
     3001 attagtagac atctcgctga taacggcgct ctacgcgcag ctcacttaaa aattcatccg
     3061 ccgcttcggt gtccatgcca ccaaattcgg caatcacttc cagaagtgcc tgctcaacgt
     3121 ctttcgccat gcgattagcg tcgccgcaga cataaatatg ggcaccgtca ttgatccagc
     3181 gccacagctc cgcgccctgt tcgcgcagtt tgtcttgtac gtaaattttt tctttttgat
     3241 cgcgcgacca ggcaagatcg atacgcgtca gcacgccctc tttgacgtag cgctgccatt
     3301 ccacctggta gaggaaatct tccgtaaagt gcgggttgcc aaagaacagc cagtttttcc
     3361 ccggcgcttc gtcggcggcg cgttgctgca taaaggcgcg gaacggcgcg atgccggtgc
     3421 ctgggccaat cataatcacc ggggtttctg ggttggctgg caggcggaag ttgtcgttat
     3481 gttcgataaa tacgcggact tcgccctctt cttccacgcg gtccgcaagg aagctggagg
     3541 caccaccggc acgggcgcgg ccttccacgt cgtaacgcac cacgccaacg gtgacgtgta
     3601 cttcgttctc gacttccgcc tgcgaggagg cgatggaata caggcgcggg gtcagcgggc
     3661 gcagcaggtt aatcagcgct tcggcatcca gttgcgccgg agagaaacgc accatgtcaa
     3721 caatcggcgt ggtcgcggcg taatgctgta acttcgcttt atcgcccacc agcggcagca
     3781 gcgtttcgct gcgggtaagc gtggcgtagt tctcaacaat attcgcggtg ttgacggtca
     3841 gttcaaagtg ccactgtagc gcttcgctca gggtcagcgt tttgccgtca acggtgacag
     3901 gttcatcgcc tttcagccac agcagttcga caagttcttt caccagtgcc ggatcgttct
     3961 gataccagac gcccagcgcg tcacccggct ggtaacgcag gccagagtca cctaagtcaa
     4021 tttcgatatg gcgaacgtct ttttcagagt tacgcccggt aattttctgg ttaaccgaaa
     4081 ggctcgccac cagcggcgcg tctttgctgt acgggctggt gtggatttca tttaccgcgc
     4141 cagtagcgac ggattgcgaa ggtgccgtga caggcgcacg cgatttaagc gcatcaacca
     4201 cgcgggcgcg ccactcgatg gcagcagcct ggtattcaac gtcggcatcg acacggtcga
     4261 gcaggcgttc accgcccagt tccgccagct tgctgtcaaa atctttcccg gactggcaga
     4321 aaaattcata agagctatcg ccgaggctaa acacggcaaa tgcagtgttt tccagctttg
     4381 gcgctttttt ggagaacagg aacttatgca gcgcgacggc ttcttccggc ggttcccctt
     4441 ccccttgcgt tgacgtcacc acgatgagca gtttttcgct ggcgatttgt ttgaatttat
     4501 agtcgcccgc gttcaccagc ttaacgttca gttttgccgc taataaatca tcgcgtaatg
     4561 cttcagcaac ccggcgcgca ttgccggttt gcgaagcgga gataatagtt atacccggca
     4621 tttctgcggc gggcgctggc gtcgctgcaa gcgcagcagg ctgctggttg agcacgcccc
     4681 agaaatagcc agaaacccag gcaagctggg taggagttaa atcggtcgtg gccgcctgaa
     4741 ggcgtgccag ttgctccggg ttcaacggaa gcaacgcgga aggtgggacc tgtgtcgtca
     4801 tgcgtcgtta tgttccagta agcaaagctg tttctgcgcc ctgtcagcgc ccataaaaca
     4861 gaagagaagg taaggttaac ggggtgaacg gtgtggatta aagacgggat agcgataact
     4921 aataaccaaa tcgactaacc tgttttagca atagtcttta acaacaaaac agattaacca
     4981 acccaatgaa aaacaaatga atttagccaa tcattaagat aaatcagcga ttttgcgcaa
     5041 caaagtcgtt ttagataatg cgaaaaaaca gcctttccgg tactctacgg cggttttatc
     5101 gtcatgtaga gaaattatga tgtccaccac gttatttaaa gatttcacct tcgaagccgc
     5161 tcaccgctta ccacacgtcc cggaagggca taaatgtggt cgcctgcacg ggcattcctt
     5221 tatggtgcga ctggaaatta ccggggaagt cgatccgcat acgggctgga ttatcgattt
     5281 cgctgaactg aaagcggcgt ttaagccaac ctacgagcgt ctcgatcacc attatctcaa
     5341 tgatattcca ggtctggaaa acccaaccag cgaaatttta gcaaaatgga tttgggatca
     5401 agttaaaccg gttgtgccgc tgttaagtgc ggtgatggta aaagaaacct gcaccgcagg
     5461 ttgtatctat cgcggcgaat gataagagtg tgtcggcggt caatttccct taagtaacgc
     5521 tatgttaggg tgttgtgttc tggatatctg gggcatgaca tggaagacga ctgcgacatt
     5581 attattatcg gtgccggaat tgctggcacc gcttgcgcgt tacgctgcgc gcgagcgggt
     5641 ttatccgttt tgttagtgga acgagcggaa attcccggca gcaaaaatct ttccggcggg
     5701 cggctataca cccatgcgct cgcggaactc ctcccacaat ttcacctgac cgcgcccctt
     5761 gaacgacgca tcactcacga aagtctttct ctgttaacgc cagatggcgc aacgacgttt
     5821 tccagcttac agcccggcgg tgaatcctgg agtgtattac gtgcacgatt cgatccgtgg
     5881 ctggttgccg aagccgaaaa agaaggtgtc gaatgcatcc ccggtgcgac ggtagatgca
     5941 ctgtatgaag aaaacggcag ggtgtgtggt gtcatttgtg gtgacgatat tcttcgcgcc
     6001 cgttatgtgg tgctggcaga aggtgccaac agcgtcctgg ctgaacgtca cgggttagtg
     6061 actcgtcctg ctggcgaagc gatggcgttg gggatcaaag aagtgctgtc gctggaaaca
     6121 tccgctattg aagaacgttt tcatctggag aataacgaag gcgcagcgtt gctgttcagc
     6181 ggcgggatct gtgatgactt acccggcggc gcatttcttt atactaatca acaaacgctc
     6241 tcgttaggga ttgtttgccc gctctcttcc cttacgcaaa gtcgtgttcc ggcaagcgag
     6301 ctgctggctc gctttaaaac gcatccggca gtgcgcccgc ttatcaaaaa cacggaatca
     6361 ctggagtatg gtgcgcatct ggtgccagaa ggtggcttgc acagtatgcc ggtgcaatac
     6421 gccggtaacg gctggctgct gctgggcgat gcgttgcgca gttgcgtcaa taccggaatt
     6481 tccgtgcgcg gcatggatat ggcgctgact ggcacgcagg cggcggcaca aacgctgata
     6541 agcgcctgcc agcaccgcga gccgcaaaat ctgtttgcgc tttatcatca caacgtcgag
     6601 cgcagcctgc tatgggatgt tctacaacgt tatcagcatg ttccggcgct tttgcaacgc
     6661 cctggatggt accgtgcgtg gcctgcgtta atgcaggata tttcccgcga tttatgggat
     6721 cagggtgata aacctgttcc accgctgcgc cagttattct ggcgtcattt acgtcgtcac
     6781 ggcctgtggc atctggcggg cgatgttatc aggagtctgc gatgtctgta gcccgtaatc
     6841 tctggcgcgt tgccgatgcg ccgcacattg taccggctga ctccgttgag cgtcagacgg
     6901 tacaacggtt gattaacgct tgtccggctg gtcttttttc gctcacaccg gaaggtgact
     6961 tacgcgttga ttatcgcggc tgcctggagt gtggcacctg ccgtttgctg tgcgacgaat
     7021 caacactaca acagtggcgc tatccgcctt ccggattcgg catcacctac cgttttggat
     7081 aaataataag gataatttat gcccctctta cacctgctcc gccagaatcc ggtgattgct
     7141 gccgttaaag ataatgccag cctgcaactg gcaatcgact ctgaatgcca gtttatttcc
     7201 gtgttgtacg gcaatatctg caccattagt caaatcgtta aaaagattaa aaatgccgga
     7261 aaatatgctt ttattcatgt tgatttgctg gaaggcgcgt caaataaaga agtggtgatt
     7321 cagtttttga aactggtgac tgaggcggac ggcattatca gtaccaaagc ctcaatgttg
     7381 aaagcggcaa gagcggaagg ttttttctgt attcatcgcc tgtttattgt tgattcgatt
     7441 tcgtttcaca acattgataa gcaagttgcg caatcgaatc cggattgtat tgagatcctg
     7501 ccaggctgta tgcctaaagt gctgggctgg gtgacggaga aaatccgcca accgctgatt
     7561 gccggcgggc tggtgtgcga tgaagaagat gcgcgtaatg cgattaacgc gggtgttgtg
     7621 gcgctttcca ccacgaatac cggggtctgg acgttagcga aaaaattgct ttgacgggat
     7681 actcgtaacg agttgaattt gtgtgatttg taggctgcac gccacatccg acattcagcg
     7741 cctgatacga cgcttgacgc gtcttatcag gcttacaagt ccccagccca gaaccgtagg
     7801 caggatcatc cggcagcacg ttattggcaa tctgcgtgaa tggttgtcac cagtgcctcc
     7861 agcaccgctt tccagtcatc aaccacgcca acatctgcct gtgaaaatac cgccgcgctg
     7921 gcgtcatgat taatcgccac cacaaacttg ctattgcgta cgcccgccat taacgctgct
     7981 gcgccggaag cgccaacaac aatacacacc tcaggcgcca gcagatgtcc ggaaatacca
     8041 atcacttttt cagcatcaac gccaccgttc atcactcgcg cccgactgta gcccacttcg
     8101 gctcccagct tttctaccag catagcgatt tcctggttat ctgcctctcc gccctgcccc
     8161 accaccagca cccgtctggc ttcagctaat ggatcgcggg taacattttt caggttttcg
     8221 acgctgataa gccagtctgg aggcgcgccg gggacaatgt tcagttgctg cataccagag
     8281 ggcaacgtgg cgtttttcag tgctcccgcc tggcgggcaa gggaaaggca tagcgggcgt
     8341 ttttctgttt gcagcgtcgc cgttagcgca tttccccagt gggatttgcg catgcttaca
     8401 gttggaatat ctaacgaagt cacctggcag atgcttgcac catgtaaacg ccaggccagc
     8461 cgggtcgaca gttcatcgcc aaacgttccc ggcggaaaga gaacaacgtc cgctggcgtt
     8521 cgctgccact gttccaccag cgcatccagc acctgttccg ccactacggg ctgaggttca
     8581 atctgccagt gcgccagggt acaaccgctg aaatcctgtg ccgccagcca gcttgctatt
     8641 gcggcattct cctgatttat tgtcacaatt gtgatattca tggctgcatc ctttggcgta
     8701 aatagttctg ccatagcttt tgcgcttttt ctgctaccgt ttgcccatca atcagcgtcg
     8761 ctccgcgtcg ttgctctgct cttgccagtt gcagacactg catcgctggc aattcggcag
     8821 caacagtttt acggataatt tctgctttcc ccgcagccat tcgctggcgc attcccggta
     8881 ctggcaaggc cacttcacca cactggcgca cggcaatcac cgcaggcaag cgaacccggc
     8941 aacagcgcaa cccatgttca gtacgctgtt cgagggtgat aaacggcgcg tcgagagtga
     9001 aacgctccac ctgggtgaaa cagggccagc ccagcatttc cgccagcaaa aatggcgttt
     9061 gtccattctg cccttcgctg ctttggcagc cggtaattat cagatccagc ggattctgac
     9121 gctgccattc ggcaatatgg cgagcgacaa attccgacgc aaagcgcaga tctgccgccg
     9181 tctccagcac tacagcttcc tcaaacccaa gagccatgag atagcgcagc cagtgcaacg
     9241 cccgttcatc ccccatgctc aacgcggtta aagacatcga cgtaccgttt tttctctgcg
     9301 ccaacagcag cgcggcggca gcctgttcat cagcaccgag taaacttcgc agaagcgaaa
     9361 catccggtcc gctattaccc tgagccgccg cctgccactc tttttccgcc agcattccgg
     9421 catccggttc ggctttaaac gctaacagaa tgttcattcg cacctcctac atttcccgcc
     9481 gccaccagcg ggagtgcttt ggtttctggt gcccatagcc aggtcacaac aaaaccaacc
     9541 aacaacactg ccgccaggag caataaggtg acttgcattc cccactgcgc cagcacccac
     9601 ggcagcaggc cagtacttac cgccgcgcca agtcgactca tggcggtggc aaaaccgacg
     9661 cccagcgagc gaatgtcagt aggaaaactt tccgcaggca aaatgcccac cagattactg
     9721 actgccgaaa tggtggtgct gaagagaaca aaaagcagca gcgttaatga actgccggaa
     9781 ggcaaacagg ccatcacgac cagcgttgcc gccagcagca aaaaacttcc cagcaaaaat
     9841 ttgcgatgtg ccagcaggtg cgtcagaact aatcccagca gcgcgcccac aattaacaac
     9901 gcattaagca tcaggctggc tgtcagcgca tcttccagac caatagtttg ggcgatggtc
     9961 ggcagccagg tataaatcac aaaccatggg attacgaggc agacaaagaa gacgctgtta
    10021 aacgctgtgc gccgccagta gcgcgaagag aacaaggttt tgatgtgttt atgggtcgcc
    10081 gttgccactt catcgcccag taaaacatgg ggaccaaaat agcgatgcac gatagcgtga
    10141 gcttctgcaa aacgcccctg gcgcagtagc cagcgtggcg attctggcgt tccccagcgt
    10201 aataacgtaa tcaacaatgc gggcagagct gccgatgcca gtagccagcg ccaggcctcc
    10261 gggctttcgc taataaagtg atgtccggca atacttgcca gcacatagcc tacggtccac
    10321 accacgctga atgcgcccag caaaacaccg cgatggcggc gcggagaaaa ttcagccagc
    10381 aaagtgtgac cgactgagta atcgcctccc agaccaatgc cgatcaaaat gcgcagtcca
    10441 ataagatgct ctggcgtggt ggcaaaaaat tgtaagaacg aagcgagcgt aatcagcaaa
    10501 aagctgaagg tgaagatttt ttgccgacca atatggtcgg agatccaccc caaaaccagg
    10561 ctaccgataa acaggccaag tagcgccgag ccgccgatca ttcccgccat aaacggcgtc
    10621 agttgcatgg cgggtgtaag ctgaataatg gcgtaaccaa tgacgccgag gacataaccg
    10681 tcggtcaggt gtgcgccgaa agtgagcgca gcaatgcggc agtgaaaacg gttaagtggt
    10741 aaatcatcca ttcgcaccgg tgaagtgttc atctttaccc cttcattgtc cggtaataag
    10801 aacggcttct cgcctgagaa gccgaaaagg caacgccccg tcatcgcgga acgctgcctg
    10861 acacattatt tttcaatcgg atagatagta cccgtattca taatgccgtt aggatcgaac
    10921 tgctttttca gcccttccag caacgcccac gcgctgccgt gttccagttt gctccagtga
    10981 acgcgatgtt taccgatacc gtggtggtgc accatcgaac caccgaggcg aatggtttct
    11041 tcgcagatga ttttgttgag cggattgtgg tacttgtcga tttcctcttc cggcttacaa
    11101 tcaacgacgt tgtagtcgta gacgaagtac atgttggtgc cgttctgata gctatgagag
    11161 gagtgaccgc ccagcatggt gatgtcgtcg gcatgcggga actcggtacg aatacggtta
    11221 ataacgcttt cgtagatttc gtggatgcag ctccagcagc cggacacttc ggtggtaaag
    11281 cccatgttgc cggttttgag gatctgcaca cgttcagcgg ccactttatc cggcccccag
    11341 ttcaggtgat taaaccaggt ttcgatcagc ttgctgtcca ctcgctggca ttgcgggtaa
    11401 cgtgccacga tttcagcaat cccttcgcct gtcgccttcg caatgcgagg gttaccttca
    11461 gccataaaga tcagcacaca tttaccatcg gcaaaatggg tgaagtgttg ggtgccatct
    11521 tcagcgtcat acaaacgagc gatcgacgga cgataccctt ccaccatgat ttcacgcagg
    11581 atgttgaagc cggttttcat gtcttccagc acatagccat agaagagatt gttttccggc
    11641 gtaaatttga agattttcac tgtgacttca gtgatatagc acagtgcacc ttcgttgcca
    11701 atgatgatat gacgaatgtc cgggccagcc gcgcgacgtg gcacgttttt aatgcgtgtg
    11761 acggtgccat ctgccagcac cgcttccaga ccaacgacca tatcctcgat tgcgccgtag
    11821 agtgtggaaa actgcccgat actgcgggtc gccaccaggc cgcccatctg cgccagcggc
    11881 tttgactgcg gagaatgccc cgtggtgtaa cctttttcac gcaacgcgtt ttccagcacc
    11941 tccagcggaa cgccacattg cgccgtggcc tgcatattct caatatcaat attaatgatt
    12001 tgattcatgg cggagccgtc gagcaccacc gagttttcca caacggtttc cagcccacct
    12061 tcggtggcgg aagcaccggt acgtggcaca ccgttaattt tgtgcgcatt cataaaattc
    12121 agcacacggg atacttgttc tgtagaaccg agttttacga ccgctgccgg aatcggcaaa
    12181 gtataaattc catgaatatc cggaaattta cgaaaacggt caatactgtt tttctttaat
    12241 accgtttcat cggtaattac gcgatctgca ccaacaattt cctttagctg gtcgacaatc
    12301 gctgcgcgag ataaagacat agtaattcct tcctgataat aaaaatggaa taatcattaa
    12361 taagagaaaa cagaaaccac cgttatggat tagcgcacta aataaccgcc atcgaccact
    12421 aataaatgcc cgttgacata attcgatgcc tgacttgcga gaaacacggc tgcgcccatt
    12481 aaatcctgag tatcgcccca acggtttgcc ggaatatgat caagaacgcg ctgattggtt
    12541 tctggattac tgcgtgtcgc cagcgtaata tctgttgcat aatagccagg ggcaatacca
    12601 tttacctgaa tattatattg acctagttca tcacaataag ctttggtgaa cccggcaaga
    12661 gcatgtttag tggcagaata tgcaggtgac cattgtccac ctaagtaaga gaacaatgaa
    12721 cagatattaa tgattttacc gcttttctgc gggatcataa tttttgctgc ttcgtagctt
    12781 aactcgaatg cggcggtcag gttcacgtca atcatcgggt cccagtcggc acgaccgaag
    12841 tccagcacct tattcagttt acaaataccg gcattgttaa ccagaatatc gacggtaccg
    12901 aagcgctcac agcaggcagc gataatcttc tgcggtgcgc cttctgcggt gatatccacc
    12961 tgcatgaagt ccacttcaac accctgtttt tcaatcattt cctttgtttc gccgttatct
    13021 ttaacgaagc ttggaataaa gatatttgcg cctgctttgg ccagcgccat ggcaaatgcc
    13081 tggcctaaac cgctattccc gccggtaaca attgcggttt tacctttcag ggagaaaaaa
    13141 tccattgaga acgcattgag agattcgatt gacatagtta gctccaattt ccgtaaggca
    13201 aaaaaaaaga aaggtaactt cccccgcgag agggaagtta cctttctcat tatctctggt
    13261 aactggagat aatttttagc ataagggcat aatcgctaat gtgatcgtaa tcacagtgtg
    13321 atattcgaat tgtcaaataa ttttaaaaag cagaagcgtg attactctca cacaacacct
    13381 ctgctttcca tgttatttct tattgcaatt actagagacc tgagaaaagt aatccacctg
    13441 aaagggcggc ttacttttct cttttttttg tctattacaa atgggaatac gatatgcaac
    13501 acaactcata tcgccgttgg ataaccctcg cgataattag ttttagcggc ggcgttagtt
    13561 tcgacctggc ttatttacgt tatatttatc aaattcccat ggcgaaattt atgggattca
    13621 gcaataccga gataggttta ataatgagta cctttggtat tgcagctatt attctttatg
    13681 cccccagcgg cgttattgcc gataaatttt cacaccgcaa aatgattact tccgcgatga
    13741 tcattaccgg attactgggt ctgttaatgg caacgtatcc accgctgtgg gtaatgctct
    13801 gtattcaggt cgcctttgcg ataacgacga ttttaatgct gtggtcggtg tcgattaaag
    13861 ccgcctcatt gctgggcgat catagtgagc aagggaaaat tatgggctgg atggaagggc
    13921 tgcgcggcgt cggtgtaatg tcgctggcgg tgtttaccat gtgggtcttt tctcgctttg
    13981 cgccggatga cagcgccagc ctgaaaacag tcattattat ctacagtgtg gtttacatct
    14041 tgttggggat tctgtgctgg ttttttgtta gcgataacaa caacctgcgc agtaccaata
    14101 acgaagaaaa acagtcattc cagcttagcg atatcctggc cgttttgcgt atcagtacca
    14161 cctggtattg cagcatggtg atttttggtg tcttcactat ctacgccatt ctgagttact
    14221 ccaccaacta tctgaccgaa atgtacggca tgtcgctggt ggcggcgagc tacatgggga
    14281 ttgtgatcaa caaaatcttc cgcgcactgt gcggcccact tggtggcatt atcaccacct
    14341 acagcaaagt gaaatcccct acccgcgtga tccaaatcct ttccgtactc ggcttgctgg
    14401 cgttaactgc cctgctcgtc acgaactcta acccgcaatc ggtcgcgatg gggattggcc
    14461 tgattttact gctgggattc acctgttacg cctcacgcgg gctgtactgg gcctgccctg
    14521 gcgaagcgag aacaccgtct tacattatgg gcaccacggt aggtatttgt tcggtgattg
    14581 gattcctgcc ggatgtcttc gtttacccga ttatcggcta ctggcaagac accctgcccg
    14641 ctgcagaagc ctaccgcaac atgtggctaa tgggtatggc ggcgcttgcc atggtgatca
    14701 tctttacctt tttgctgttc caaaaaattc gtactgctga tagcgccccc gcaatggcta
    14761 gcagcaagta agccaggaaa attacaggag aaaaatcatg tcgaaaaaat acatcatagg
    14821 gattgatggc ggaagtcaga gcacaaaagt ggtgatgtac gatctggaag gtaatgtggt
    14881 ttgcgaaggc aaaggcttat tgcagccgat gcacacgcca gatgccgata ccgcagaaca
    14941 tcctgacgat gatttatggg catcactatg ttttgccagt cacgatttga tgagccagtt
    15001 tgccgggaat aaagaagata ttgtcggcat tggcctgggc tcaatccgtt gctgccgtgc
    15061 gctattgaaa gccgatggta cgccagctgc gccgttgatt agctggcagg atgcacgcgt
    15121 tacacgccct tacgaacata cgaatcctga cgtggcatat gtcacctctt tttcgggtta
    15181 tctgacgcat cgcttaaccg gcgagtttaa agacaatatc gccaactatt ttggtcagtg
    15241 gccggtggat tataagacct gggcatggag cgaagatacc gcggtaatgg agaagtttaa
    15301 tattccccga cagatgcttt ttgatgtgca aatgccgggc actgttcttg ggcatatcac
    15361 tcgtcaagcc gcgctggcga cacatttccc ggctgggctg ccggttgttt gtaccaccag
    15421 tgataaaccg gtagaagctc tgggagccgg gttactggat gatgaaacgg cggtaatttc
    15481 tcttggcacc tatatcgcgt tgatgatgaa cggcaaagca ctaccgaaag atccggtggc
    15541 gtactggccg attatgtctt ctattccgca aacattgctg tatgaaggtt atggtattcg
    15601 caaaggcatg tggacggtga gctggctgcg cgacatgtta ggcgagtcgt taattcagga
    15661 tgccaaagca caggatcttt caccggaaga tttgctcaac aaaaaagcgt cttgcgtgcc
    15721 acctggttgt aatgggctga tgacggtgct ggactggctg accaatccgt gggaaccata
    15781 caaacgcggg attatgatcg gctttgattc cagcatggat tacgcatgga tatatcgttc
    15841 gatactggaa agcgtggcgc tgacgctgaa gaacaattac gacaatatgt gtaatgaaat
    15901 gaatcacttt gcaaagcatg tgatcattac tggcggcggt tcgaacagcg atctgtttat
    15961 gcagattttt gccgacgtgt tcaaccttcc ggcacgccgt aacgccatta atggttgtgc
    16021 aagtctgggg gcagcgatca atacggcggt aggtctgggg ctatacccgg attacgcaac
    16081 ggctgtcgac aaaatggttc gcgtgaaaga tatctttatg cctgttgaga gcaacgccaa
    16141 acgctacgac gcgatgaata aaggcatttt caaagaccta accaagcaca ctgatgtgat
    16201 cctgaaaaaa tcgtatgaag tgatgcatgg ggaattgggg aatgcggatt cgatccagag
    16261 ctggtcgaat gcgtaagtga ggaaggccgg gcgggaaaat gcccggcctg aacatacctg
    16321 aatggttatc cccgctgacg cggggaacat aagttcgaca acttaaaaaa tgataacaag
    16381 tccggtttat ccccgctgat gcagggaaca taataaaaac tttaactagt tcggtttatc
    16441 cctgttttta caaggaatat taactacact cgatgtcttt agaataaaag acaatacgaa
    16501 tcgcacttta tcaattttca cttaaagtat gaactggata tctttattca taagaactat
    16561 tcatcaacag cctatagagg cggttatgta aaaccactca ttagcctcaa aacaaagaca
    16621 aatttccaga tttattactt
//
LOCUS       DAPHCV010000104        16246 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.104, whole genome shotgun sequence.
ACCESSION   DAPHCV010000104 DAPHCV010000000
VERSION     DAPHCV010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.104"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            838..1137
                     /locus_tag="RH033_003596"
     CDS             838..1137
                     /locus_tag="RH033_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992725.1"
                     /translation="MTVVITYLADDNARNRRRARRQAQREQAMQEQRLARKIALKLSG
                     CVRADKAASLGSLRCKKAEEVESKQNRIYYRKPRSEMGVTCVGRQKMKLSSKPLI"
     gene            1146..1616
                     /locus_tag="RH033_003597"
     CDS             1146..1616
                     /locus_tag="RH033_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992726.1"
                     /translation="MTKSWSVPFPESETEHDGMPVFWRFQATVEEDGIKIFALQYIAF
                     HQTEHYAWLVPAHWIVNFKPAPNQWLQEWKQRRNRYAIKKVAKNAERSFAFPTKKLAI
                     ESLLRRKKYHLMRIKQDLAVVSTLVDGMKNIDTSTPDIEYNFGHNQETENWVFY"
     gene            1663..1941
                     /locus_tag="RH033_003598"
     CDS             1663..1941
                     /locus_tag="RH033_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992727.1"
                     /translation="MKEFKGTPGKWKYTVRNVNEMMTTFHGVTIGDTYIEAATRNERE
                     DALLIAAAPDLLEALQELVFLYEHDEGCRELTEYKRAKAAISKALGEE"
     gene            1938..2099
                     /locus_tag="RH033_003599"
     CDS             1938..2099
                     /locus_tag="RH033_003599"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992728.1"
                     /translation="MMNKKYIVEVIERETKEVIKHFEFDNYRKADRVEEGLLRQSNLE
                     KFDVVMRCE"
     gene            2279..2404
                     /locus_tag="RH033_003600"
     CDS             2279..2404
                     /locus_tag="RH033_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HDV2992729.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRVEVLLCAMK"
     gene            2389..2541
                     /gene="kil"
                     /locus_tag="RH033_003601"
     CDS             2389..2541
                     /gene="kil"
                     /locus_tag="RH033_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HDV2992730.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINL
                     PWYRRK"
     gene            2798..3403
                     /locus_tag="RH033_003602"
     CDS             2798..3403
                     /locus_tag="RH033_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HDV2992731.1"
                     /translation="MSKEFYARLAAIQENLNAPKNQYNSFGKYKYRSCEDILEGVKPL
                     LNGLFLSISDEVVLIGDRYYVKATATITDGENSHTATALAREEESKKGMDSAQVTGAT
                     SSYARKYCLNGLFGIDDAKDADTDEHKHQQNAAAKQSKPSPTPEQVLKAFTDAAMQKN
                     TVEELKQAFAKAWKMLEGTPEQHKAQDVYNIRRDELEGAAA"
     gene            3403..3786
                     /locus_tag="RH033_003603"
     CDS             3403..3786
                     /locus_tag="RH033_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992732.1"
                     /translation="MAHSITVRLNKPAREFQAGENIGFNIRAGVQYYDRQTKKKEWTN
                     YSAVVFAKPGAQEDYYRSVLVEGGIVEITGENIRVDVYQGQNGQSITLELLNAKIGFA
                     TSGNSQQQQSSNHQNNPEYDDSIPF"
     gene            3810..4103
                     /locus_tag="RH033_003604"
     CDS             3810..4103
                     /locus_tag="RH033_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2856 family protein"
                     /protein_id="HDV2992733.1"
                     /translation="MPAPLYGADDVRRCSGNSVSEVLDKFRKNYDRIMSLPQETKEEK
                     EFRHCIWLAEKEERERIYQTSIRPFRKATYTHFTEIDPRLRNYRSRYGAISND"
     gene            4114..4281
                     /locus_tag="RH033_003605"
     CDS             4114..4281
                     /locus_tag="RH033_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HDV2992734.1"
                     /translation="MRGLAYNPGILPAEMIIRQRVKPMPSREELLKRNSFPSVNQNKY
                     LNAMWRKGGKQ"
     gene            4278..4577
                     /locus_tag="RH033_003606"
     CDS             4278..4577
                     /locus_tag="RH033_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992735.1"
                     /translation="MSVCLIDKRRRGQQIPSVGMPNHTWFCVLDIDGMDALVDTRHYC
                     DTATATPAKAKKMAALIENWTPPDGWCNGNDRDWHEKMKGYICDFLRKCNGFRVM"
     gene            4579..5145
                     /locus_tag="RH033_003607"
     CDS             4579..5145
                     /locus_tag="RH033_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HDV2992736.1"
                     /translation="MNKIDYQALRAKAEKATCGEWSLEYGEERFNADDALIHREVAGY
                     IPICRIEGAHPESGFDEDFQTEQQANAEFIAAANPATVLALLGELEAAKKRIAELEAE
                     LVSQTYKLNELSGNSPVTPDGWISCSERMPDTKTAVLVAVEFDRKGDWRMKWATYIPG
                     HPDANDGWIIPGASWKPSHWMPLPEPPL"
     gene            5242..5421
                     /locus_tag="RH033_003608"
     CDS             5242..5421
                     /locus_tag="RH033_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eag protein"
                     /protein_id="HDV2992737.1"
                     /translation="MSCPKCGSGNIAKEKTMRGWSDDYVCCDCGYNDSKDAFGERGKN
                     DFVKINKEREGNEKS"
     gene            5522..5866
                     /locus_tag="RH033_003609"
     CDS             5522..5866
                     /locus_tag="RH033_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992738.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKNNIYGHHFGAPH
                     DAADFRRCVSLVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            5990..6190
                     /gene="torI"
                     /locus_tag="RH033_003610"
     CDS             5990..6190
                     /gene="torI"
                     /locus_tag="RH033_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HDV2992739.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            6487..6639
                     /locus_tag="RH033_003611"
     CDS             6487..6639
                     /locus_tag="RH033_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992740.1"
                     /translation="MHSLMNSQARDTRDTSLNDDNVLEISEPRIRSTFERLYQNMNEA
                     FGLGDY"
     gene            complement(6719..7966)
                     /locus_tag="RH033_003612"
     CDS             complement(6719..7966)
                     /locus_tag="RH033_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2992741.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMRFKDKDHQCIAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(8038..8952)
                     /locus_tag="RH033_003613"
     CDS             complement(8038..8952)
                     /locus_tag="RH033_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HDV2992742.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDWDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            9168..10601
                     /gene="cscA"
                     /locus_tag="RH033_003614"
     CDS             9168..10601
                     /gene="cscA"
                     /locus_tag="RH033_003614"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDV2992743.1"
                     /translation="MTQSRLHAAQNALAKLHERRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDENDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDSGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSISPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQGDMLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWQLG"
     gene            complement(10609..11667)
                     /locus_tag="RH033_003615"
     CDS             complement(10609..11667)
                     /locus_tag="RH033_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV2992744.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEDR
                     EGGRVTRIPCPLLIRCST"
     gene            11847..12065
                     /locus_tag="RH033_003616"
                     /pseudo
     CDS             11847..12065
                     /locus_tag="RH033_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            12083..13411
                     /gene="dsdA"
                     /locus_tag="RH033_003617"
     CDS             12083..13411
                     /gene="dsdA"
                     /locus_tag="RH033_003617"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HDV2992745.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GSQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(13519..15057)
                     /gene="emrY"
                     /locus_tag="RH033_003618"
     CDS             complement(13519..15057)
                     /gene="emrY"
                     /locus_tag="RH033_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HDV2992746.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLVAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(15057..16220)
                     /gene="emrK"
                     /locus_tag="RH033_003619"
     CDS             complement(15057..16220)
                     /gene="emrK"
                     /locus_tag="RH033_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HDV2992747.1"
                     /translation="MEQINSNKKYSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPELASTVTSMPAYTSKALVIDTTPIEKEISNIISHNGQL"
BASE COUNT         4838 a         3715 c         3710 g         3983 t
ORIGIN      
        1 acagatctaa ttgaacagta taaaatacct accccaaacg agagaaaagc aatagatcaa
       61 tatcgtattg acttagaaat cgacgaaatg ctaagcaaaa gaaaagacta aaaacacccg
      121 gcctcagcgc cgggttttct tttcctgccg ttcaccaccc aatcaaccat cctcatcata
      181 gacaagcatc aagccaaagg taacaccttc accccgcacg taagccctca aacaccaatc
      241 aatcagcaac atttacaaaa ataaaatacc tttgttatcc atcacttata acttatttac
      301 cataaaatat aaattaggtt attgaccaca cctataacct aagttatctt taagccatca
      361 gcaggacgct ggaagccaaa cggaacagct tggcaggctc tttaacattg atgggattgt
      421 cccgccgaaa tgcgggaacc aaagagtagt tggctttggg attggatgaa tgagcaggct
      481 gatgctcgac caatgtataa acagcgctca tggcaagcag taaccaatct gcgcctcaag
      541 acagcgtcac tggtagtgcg ggcgctctaa ccagtaagcc ggagttcagc accggccatc
      601 caatcgccaa agtcaattcc ataggcgtta tgcagccgcc accatattca agaaagctgc
      661 acaagaggta ggaggattta tgtgaatact tacattcaat tgagagatta attaaataga
      721 actgatcgag cagagtccat aaaggctcat aaatgctctt ccatccccgt ctaattggcg
      781 gggatggaaa ccactttgta ataaaaaaat tccaaagttg tttcatcgga ggtcaacatg
      841 acagtagtca ttacatatct ggctgacgat aacgccagaa atcgccgcag agcacgcaga
      901 caggctcaac gtgaacaggc aatgcaagag cagcgactgg cgcgaaaaat tgcgctaaag
      961 ctctctggtt gcgtcagagc agataaagca gcatcactcg gaagccttcg ctgcaagaag
     1021 gcagaagaag tcgagagtaa acagaatcga atttactacc gcaagccacg cagtgaaatg
     1081 ggtgtgactt gtgttggtcg ccagaaaatg aaattaagca gcaaaccact tatttgaggt
     1141 gagatatgac aaaatcatgg agcgtacctt ttcctgaatc agaaactgaa catgatggaa
     1201 tgcctgtttt ctggagattc caggcgacag ttgaagaaga tgggataaaa atattcgcac
     1261 ttcaatatat agcttttcat cagacagagc attatgcatg gttggttcct gcgcattgga
     1321 ttgttaattt taaaccagca ccaaatcagt ggttacagga atggaaacaa aggagaaata
     1381 gatatgcaat taagaaagta gcaaaaaatg cagaaagatc ttttgcattc ccaacgaaga
     1441 aacttgccat tgagagttta ttgcgccgga agaaatacca tttaatgaga atcaaacaag
     1501 atttggctgt tgtatcaact cttgttgatg ggatgaagaa tattgataca tcaacaccag
     1561 atattgaata taactttgga cacaaccaag aaacagaaaa ttgggtattc tactaggccg
     1621 catagtcggc ctttattttt ggcataaaca acagaggtgg atatgaaaga gtttaagggt
     1681 acgcctggta aatggaagta cacggttaga aacgtcaacg agatgatgac tacgttccat
     1741 ggtgtgacga ttggtgacac atacattgaa gcagcaacaa gaaatgaaag ggaggatgcg
     1801 ctactgatag cagcagctcc tgatttgctt gaagcactgc aagagcttgt cttcctttac
     1861 gagcatgacg aagggtgcag agagttaacc gaatacaaac gagccaaggc agccatcagc
     1921 aaggccctgg gggaagaatg atgaataaga aatacatcgt tgaagttata gagcgagaaa
     1981 cgaaagaagt aattaaacat ttcgaatttg ataattatag aaaagctgac cgcgtagaag
     2041 aaggattgtt gcgacaaagt aatctcgaaa aatttgatgt tgtcatgcga tgcgaataag
     2101 cgcctatagc agatttacga gtctgctatg tgagcaatgt cgctcgtaac taaacaggag
     2161 ccgacttgtt ctgattattg gaaatcttct ttgccctcca gtgtgagggt ctttttatat
     2221 gcataccaat aacgcttcac tcgaggcgtt ttcgttatgt ataaataagg agcacaccat
     2281 gcaatatgcc attgcagggt ggcctgttgc tggctgccct tccgaatctt tacttgaacg
     2341 aatcacccgc aaattacgtg acggatggaa aagagttgag gtcttattat gcgcaatgaa
     2401 atagccatta atcaccagat gcttcgtgct gcacaaaaca aagcagtaat agccagattt
     2461 attggtgatt caaaaatgtg gcttgaagca aataaagcga tgaaatcagc tatcaacctt
     2521 ccgtggtatc gcaggaaatg agttttacag ataactggtc agacgaagaa ttcattcgtc
     2581 agatgaaaga attaatcggt aacgaaggag atattcatgt cacttgcaac cacagtgaag
     2641 gagagcaagt tacagagacg catgtacacg cagaaagctc tctggtatcg ccataatggc
     2701 gaccgcgaag gaatgcgggt atgccttaat ttgtcccgag tagaagtatt aaatcagcgt
     2761 tatttccttg ggccatgtcc attctgagaa caatcatatg agcaaagaat tttacgcaag
     2821 actggcagct attcaggaga atctgaacgc gccaaagaat cagtacaact cattcggcaa
     2881 atataaatac agaagctgcg aagatattct tgaaggcgtt aagccgttac tgaatggtct
     2941 gtttttatca atcagcgatg aagttgtgtt gattggtgat cggtattacg tgaaagccac
     3001 ggcaactatt accgatggcg aaaacagtca tacggcaacc gctcttgcac gagaggaaga
     3061 aagcaagaaa ggaatggatt ctgcacaagt tacgggagct acaagctctt atgcacgcaa
     3121 gtattgcctc aatggtttgt tcggcattga tgatgcgaaa gatgcagata ccgacgagca
     3181 taaacatcag cagaacgcag cagcaaagca gtcaaaacca tcacctacac ctgaacaggt
     3241 tctaaaagca ttcactgacg cagcaatgca gaaaaacacc gtagaagagc ttaaacaggc
     3301 gttcgccaaa gcatggaaga tgctcgaagg cacaccggag cagcacaaag cgcaggacgt
     3361 ttacaacatc agacgagacg aattagaagg agcggctgct taatggcaca ttcgattact
     3421 gtaagactaa acaagcccgc aagagagttt caggccgggg aaaatatcgg attcaacatc
     3481 cgtgctggcg ttcagtatta cgatcgccag acaaaaaaga aagaatggac aaactacagc
     3541 gccgttgtat ttgccaagcc gggagcgcaa gaggattact accgtagtgt tcttgttgaa
     3601 ggtggaattg tggaaattac cggagaaaac atcagggttg atgtttatca ggggcaaaat
     3661 ggtcaatcaa tcactcttga attactgaat gcaaagattg gatttgcaac ttcaggaaac
     3721 agccaacagc aacaaagtag caatcatcaa aataatcctg aatacgacga ttccatccca
     3781 ttctgattta gaaaaataag gatttaatca tgccagcgcc tctgtatggt gcggatgacg
     3841 tgcgccgctg ttccggcaat tccgtatcgg aggtgctgga taaattcaga aaaaactacg
     3901 accggataat gtcgctaccg caggaaacaa aagaggaaaa ggaatttcgc cactgtatat
     3961 ggcttgcaga gaaagaagaa cgcgagcgaa tttaccagac atcaatccga ccattccgca
     4021 aagccacata tacccacttc actgaaattg acccgcgcct gcgtaattac cgctcacgct
     4081 atggcgctat cagtaatgac tgaggaattt accatgagag gacttgcata caatcccggc
     4141 attcttccag cagaaatgat tattcgccaa cgcgtaaagc caatgccatc gagagaggaa
     4201 ttgcttaaga gaaattcttt tccgtcagta aatcaaaaca aatatctgaa tgcgatgtgg
     4261 cgtaaaggag gcaagcagtg agtgtatgtc ttattgataa acgtcgacgt gggcaacaaa
     4321 taccatctgt tggaatgccg aatcacacat ggttttgcgt acttgatatc gatggtatgg
     4381 atgcgttggt tgacactcgt cattactgcg ataccgcaac agctactccg gcgaaagcaa
     4441 agaaaatggc tgctctgata gaaaactgga ctccacctga tggttggtgc aatgggaatg
     4501 atcgagattg gcatgaaaaa atgaagggct atatctgcga tttcttacgt aaatgcaacg
     4561 gattcagggt gatgtgacat gaacaagatt gactatcagg cactgcgtgc caaggcagaa
     4621 aaagcaacgt gtggtgagtg gtcgctcgaa tatggagagg agagatttaa tgctgatgat
     4681 gcgctaattc atcgtgaagt tgctggatat attcccattt gcagaattga aggagcgcat
     4741 cctgaaagcg gtttcgatga agatttccaa actgaacagc aggccaatgc tgaattcatc
     4801 gccgcagcca atccggctac cgtcttggcg ctgcttggcg agctggaagc agcaaaaaag
     4861 cgcatagcag aactggaagc cgaactcgta agccaaactt acaagttgaa cgagctatcg
     4921 ggcaactctc cggtaactcc ggatggttgg ataagctgta gtgagcgaat gccggatacc
     4981 aaaacagccg ttcttgttgc cgtggagttt gacaggaaag gtgactggcg aatgaaatgg
     5041 gcgacttaca tcccggggca tcctgacgct aatgatgggt ggataattcc tggtgcgtcg
     5101 tggaaaccgt cacactggat gccgctacca gagcctccac tttgaaagcg aagcttatac
     5161 atatctttta catcagcaat ctattgttaa tctccaatca atgttacgtt gtcatctctc
     5221 tcatgctttg gaggtagtga tatgtcttgt ccaaaatgcg gttctggaaa tattgcaaaa
     5281 gaaaaaacaa tgcgtggatg gtctgatgat tatgtgtgct gcgattgcgg atacaacgac
     5341 tctaaagacg catttggaga gcgtggtaaa aacgattttg tcaaaattaa taaagaacgc
     5401 gaaggcaacg aaaaaagcta atttatttat tcatatatga aaacaatgta accaatattc
     5461 gaattgaaga actgaaagaa caccaagccg cctgatggcg gttttttatt ggagacaaga
     5521 aatgtcagat ttggctatga aggttttgaa atggcaatcg actggcgatg tcggcatcag
     5581 tagcgcaact cttgcctcaa tcgcatgtgg actgaaaaat aatatctatg gtcatcactt
     5641 cggcgctcca catgacgcag cagatttccg acgatgcgtt tcacttgttg agcagattcc
     5701 agaaatcaga gattcattcg acaaggttgc aaagcgcgtt ccggcattca aaggaatcct
     5761 caacgaatgg gattcactcg ttgcgctgtt gaagtctgaa atgaagacgt acgggaacaa
     5821 agcaccagag acttacagaa gaatcagcga gctacgcaag gactaaccca cctcacactc
     5881 gatgaggcct gttcattgct caatgatatc cagacctacc atcgccgcat caatgcggct
     5941 ttttcttgcc tgatttgcag gttcgattcc ctattcggag atagcactca tgcaacacga
     6001 actacaacct gattcactgg ttgatttgaa attcatcatg gccgatactg gctttggtaa
     6061 aaccttcatc tatgaccgga ttaagtccgg cgacctgcca aaagccaaag ttatccacgg
     6121 gcgagcaaga tggttatatc gtgaccattg tgaattcaaa aataagctct taagccgcgc
     6181 caatgggtaa aatagcgggt aaaatatttc tcacatctaa aaaataccat tccaatcaat
     6241 cccctgccgc ttcaagtaga tgtctgcagg ggacaccaga tacccttcaa accaaatcta
     6301 ccttcacccc gtaaaagatg ggtttggcag cacacttgcc ctatatctac tcatttttac
     6361 tgcaacaggt tgaaatctca gcactgtcag aaagcgctga tgactaaaca gccctggccg
     6421 ggcgatgtaa ccatcataca gaatcctgat agcgaaatat ggcgtgactc gatacttcac
     6481 tctgcaatgc attccttgat gaattcgcag gcccgtgata cacgggacac gtcgctgaat
     6541 gacgacaatg tcctggaaat cagcgaaccg cgtatccgga gtacatttga gcgactgtac
     6601 cagaacatga atgaggcgtt tggattaggt gattattagc aggggtaagc atttttgtat
     6661 tattattttc cggttgaggg atatggagat atcgacaaca accggaaaaa gtttacgtct
     6721 atattgctga aggtacaggc gtttccataa ctatttgctc gcgtttttta ctcaggaaga
     6781 aaatgccaaa tagcaacatc aggcagacaa tacccgaaat tgcgaagaaa actgtctggt
     6841 agcctgcgtg gtcaaagagt atcccagtcg gcgttgaaag cagcacaatc ccaagcgaac
     6901 tggcaatttg aaaaccaatc agaaagatcg tcgacgacag gcgcttatca aagtttgcca
     6961 cgctgtattt gaagacggat atgacacaaa gtggaacctc aatggcatgt aacagcttca
     7021 ctaatgaaat aatccagggg ttaacgaaca gcgcgcagga aaggatacgc aacgccataa
     7081 tcacaacacc gataagtaat gcattttttg gccctacccg attcacaaag aaaggaataa
     7141 tcgccatgca cagcgcttcg agtaccacct ggaatgagtt gagataacca tacaggcgcg
     7201 ttcctacatc gtgtgattcg aataaacctg cataaaagac aggaaagagt tgttgatcaa
     7261 aaatgttata gaaagaccac gtccccacaa taaatatgac aaaaacccag aagtttcgat
     7321 ccttgaaaac tgcgataaaa tcctcttttt ttacccctcc cgcatccgcc gctatgcact
     7381 ggtgatcctt atctttaaaa cgcatgttga tcatcataaa tacagcgcca aatagcgaga
     7441 ccaaccagaa gttgatatgg ggactgatac taaaaaatat accagcaaag aacgcgccaa
     7501 tagcatagcc aaaagatccc caggcgcgcg ctgttccata ttcgaaatga aaatttcgcg
     7561 ccattttttc ggtgaagctg tcaagcaaac cgcatcccgc cagataccct aggccaaaaa
     7621 agagcgcccc cagaattaga cctacagaaa aattgctttg cagtaacggt tcataaacgt
     7681 aaatcataaa cggtccggtc aagaccagaa tgaaactcat acaccagatg agcggtttct
     7741 tcagaccgag tttatcctga acgatgccgt agaacatcat aaatagaatg ctggtaaact
     7801 ggttgaccga ataaagtgta cctaattccg tccctgttaa tcctagatgt cctttcagcc
     7861 aaatagcgta taacgaccac cacagcgacc aggaaataaa aaagagaaat gagtaactgg
     7921 atgcaaaacg atagtacgca tttctgaatg gaatattcag tgccataatt acctgcctgt
     7981 cgttaaaaaa ttcatgtcct atttagagat aagagcggcc tcgccgttta cttctcacta
     8041 ttccagttct tgtcgacatg gcagcgctgt catcgcccct ttcgccgtta ctgcaagcgc
     8101 tccgcaacgt tgggcgagat cgataattcg tcgcatttct ctttcatctg tagataatcc
     8161 cgtagaggac agacctgtga gtaacccggc aacgaacgca tctcccgccc ccgtgctatc
     8221 gacacaattc acagacattc cagcaaaatg gtgaacttgt cctcgataac agaccaccac
     8281 cccttctgca cctttagtca ccaacagcat ggcgatctca tactcttttg ccagggcgca
     8341 tatatcccaa tcgttctgtg tttttccact gataagtcgc cattcttctt ccgagagctt
     8401 gacgacatcc gccagttgta gcgcctgccg caaacacaag cggagcaaat gctcgtcttg
     8461 ccatagatct tcacgaatat tgggatcgaa gctgacaaaa cctccggcat gccggatcgc
     8521 cgtcatcgca gtaaatgcgc tggtacgcga aggctcggca gacaacgcaa ttgaacagag
     8581 atgtaaccat tcgccatgtc gccagcaggg caagtctgtc gtctctaaaa aaagatcggc
     8641 actggggcgg accataaacg taaatgaacg ttctccttga tcgttcagat cgacaagcac
     8701 cgtggatgtc cggtgccatt catcttgctt cagatacgtg atatcgacac cctcagttag
     8761 cagcgttctt tgcattaacg caccaaaagg atcatcaccg acccgaccta taaacccact
     8821 tgttccgcct aatctggcga ttcccaccgc aacgttagct ggcgcgccgc caggacaagg
     8881 cagtagccgc ccgtctgatt ctggcaagag atctacgacc gcatccccta aaacccatac
     8941 tttggctgac atttttttcc cttaaattca tctgacttac gcatagtgat aaacctcttt
     9001 ttcgcaaaat cgtcatggat ttactaaaac atgcatattc gatcacaaaa cgtcatagtt
     9061 aacgttaaca tttgtgatat tcatcgcatt tatgaaagta agggacttta tttttataaa
     9121 agttaacgtt aacaattcac ccaatttgct taaccaggat gataaaaatg acgcaatctc
     9181 gattacatgc ggcgcaaaac gcactagcaa aacttcacga gcgccgaggt aacactttct
     9241 atccccattt tcacctcgcg cctcccgccg ggtggatgaa cgatccaaac ggcctaatct
     9301 ggtttaacga tcgttatcac gcgttttatc aacatcaccc gatgagcgaa cactgggggc
     9361 caatgcactg gggacatgcc accagcgacg atatgatcca ctggcagcat gagcctattg
     9421 cgctagcgcc aggagacgag aatgacaaag acgggtgttt ttcaggtagt gctgtcgatg
     9481 acaatggtgt cctctcactt atctacaccg gacacgtctg gctcgatggt gcaggtaatg
     9541 acgatgcaat tcgcgaagta caatgtctgg ctaccagtcg ggatggtatt catttcgaga
     9601 aacagggtgt gatcctcact ccaccagaag gcatcatgca cttccgcgat cctaaagtgt
     9661 ggcgtgaagc cgacacatgg tggatggtag tcggggcgaa agatccaggc aacacggggc
     9721 agatcctgct ttatcgcggc agttcattgc gtgaatggac tttcgatcgc gtactggccc
     9781 acgctgatgc gggtgaaagc tatatgtggg aatgtccgga ctttttcagc cttggcgatc
     9841 agcattatct gatgttttcc ccgcagggaa tgaatgccga gggatacagt taccgaaatc
     9901 gctttcaaag tggcgtaata cccggaatgt ggtcgccagg acgacttttt gcacaatccg
     9961 ggcattttac tgaacttgat agcgggcatg acttttatgc accacaaagc tttttagcga
    10021 aggatggtcg gcgtattgtt atcggctgga tggatatgtg ggaatcgcca atgccctcaa
    10081 aacgtgaagg ctgggcaggc tgcatgacgc tggcgcgcga gctatcagag agcaatggca
    10141 aacttctaca acgcccggta cacgaagctg agtcgttacg ccagcagcat caatctatct
    10201 ctccccgcac aatcagcaat aaatatgttt tgcaggaaaa cgcgcaagca gttgagattc
    10261 agctgcagtg ggcgttgaag aacagtgatg ccgaacatta cggattacag ctcggcactg
    10321 gaatgcggct gtatattgat aaccaatctg agcgacttgt tttgtggcgg tattacccac
    10381 acgagaattt agatggctac cgtagtattc ccctcccgca gggtgacatg ctcgccctaa
    10441 ggatatttat cgatacatca tccgtggaag tatttattaa cgacggggag gcggtgatga
    10501 gtagccgaat atatccgcag ccagaagaac gggaactgtc gctctatgcc tcccacggag
    10561 tggctgtgct gcaacatgga gcactctggc aactgggtta acataatatc aggtggaaca
    10621 acggatcaac agcgggcaag ggatccgcgt cactcttccc ccttcacgat cttcaataat
    10681 atgcaatgca gcttcccgcc cgataatgtc atgtggaagc tgaattgtgg tcagcggcgg
    10741 taaaaacaga tgcccgacgc caaccagatt atcaaagccc attacggcga catcctgcgg
    10801 gatacgtacc cccttcgcca ggagaacctg ataagcgaca aaggctgcgc gatcgttacc
    10861 acatatcaga acatcaaaat ctggtttgcc agatttgaag tgggcattga gtaaacttgc
    10921 gagatcggtg tagtgatcat cacctgttgc catgtgaaat tgtttcacct cagccagatc
    10981 tcgtccagca tcacgccagg cctgctcaaa tccctgccga cgataccctg ttgccaacgc
    11041 actttccggt agccagaagc ataacggttg acgatagccc gccgcgagca aatgctgtgt
    11101 tgattcatat tgtgcagtgt aatcatcagg gatataactg ggtaacgctg ggtcatccgc
    11161 cacacagttc gccaatacaa tattttcacc atacagagac tcaggcagcg tgatatgtcg
    11221 cagccccatt gtagtataga taatgccatc cggacggtgg gcaagcagct gacgtgcagc
    11281 acgggcagcg tcatcttcag aaaaaatatt gattaaaaaa ctattccagc cgaactcgct
    11341 ggcggtttgc tcaatggcaa gcagaatatc aacagagaaa ggagtggtgg ccgtgtcctg
    11401 cgccagcacg gcgagagtcg acggcttacg tccttgagcg cgcatcttac gggcggaaag
    11461 atcaggaaca taattcaggg tctggattgc ctgcaatacg cggtcacgcg ttgcaggacg
    11521 cacagattct gcattatgca tcacccggga gactgtcatc atcgacactc ccgccaggcg
    11581 tgcgacatcc tttaatgaag ccatacccaa gccgtttgcc gtaaaacggg cactgtagca
    11641 gaaacagacg tcactggcga gatccaacga tattctcaac gccctatcac ccgacacggc
    11701 aatacaataa aaaataacaa taattcccgg acaattgtac ccaattccgc ctctgttctc
    11761 gccaacgagt aaccatgtag gcaagtaggt aaaacatgcc aactggcatc gccaacctgg
    11821 agaagaaaat ataaatatgg atcattgtgc tgccgctgta tcccgacatc agtccggaaa
    11881 ttattgcgat tgctatcggt tcaggtgcga ttggctgcac gatcgttacg gactcgcttt
    11941 tctggctggt gaagcaatat tgcggcgcta cgctcaatga aacatttaaa tactatacga
    12001 cagcgacatt tatcgcttca gtcgtcgctc tggcgggcac attcctgctg tcatttatca
    12061 tctaagcgca aagagacgta ctatggaaaa cgctaaaatg aactcgctca tcgcccagta
    12121 tccgttggta aaggatctgg ttgctcttaa agaaaccacc tggtttaatc ctggcacgac
    12181 ctcattggct gaaggtttac cttatgttgg cctgaccgaa caggatgttc aggacgccca
    12241 tgcgcgctta tcccgttttg caccctatct ggcaaaagca tttcctgaaa ctgctgctac
    12301 tggggggatt attgaatcag aactggttgc cattccggct atgcaaaaac ggctggaaaa
    12361 ggaatatcaa caaccgatca gcgggcaact gctactgaaa aaagatagcc atttgcccat
    12421 ttccggctcc ataaaagcac gcggcgggat ttatgaagtc ctggcacatg cagaaaaact
    12481 ggctctggaa gcggggttgc tgacgcttga agatgactac agcaaactgc tttctccaga
    12541 gtttaaacag ttctttagcc aatacagcat tgctgtgggc tcaaccggaa atctggggtt
    12601 atcaatcggc attatgagcg cccgcattgg ctttaaggtg acagtacata tgtctgctga
    12661 tgcccgggca tggaaaaaag cgaaactgcg cagccatggc gttacggtcg tggaatatga
    12721 gcaagattat ggtgttgcag tcgaggaagg acgtaaagca gcgcaatctg acccgaactg
    12781 tttctttatt gatgacgaga attcccgcac gttgttcctt gggtattccg tcgcaggcca
    12841 gcgtcttaaa gcgcaatttg cccagcaagg ccgtatcgtc gatgctgata accctctgtt
    12901 tgtctatctg ccgtgtggtg ttggtggtgg tcctggtggc gtcgcattcg gacttaaact
    12961 ggcgtttggc gatcatgttc actgcttttt tgccgaacca acacactccc cgtgtatgtt
    13021 gttaggcgtc cataccggat tacacgatca gatttctgtt caggatattg gtatcgacaa
    13081 ccttaccgca gcggatggcc ttgcagttgg ccgcgcatca ggctttgtcg ggcgggcgat
    13141 ggagcgtctg ctggatggct tctataccct tagcgatcaa accatgtatg acatgcttgg
    13201 ctggctggcg caggaagaag gtattcgtct tgaaccatcg gcactggcgg gtatggccgg
    13261 gtctcagcgc gtgtgcgcat cagtaagtta ccaacagatg cacggtttca gcgcagaaca
    13321 actgcgtaat gccactcatc tagtgtgggc gacgggaggt ggaatggtgc cggaagaaga
    13381 gatgaatcaa tatctggcaa aaggccgtta ataacgtttc aacgcagcat cgccatcctt
    13441 tccctgggtg agcgatgctg ccgatgacgc agacttaaga ttctgccgtt ttacctgcta
    13501 tagcctcctc tttttccatc acccaacgcc tttcgctgta aacggcggtt tcgcaaacca
    13561 aaccaaaacc gtaagtaaga taaaagcaat agccgccata cggaaaatct catttgcaga
    13621 aatagaaagt gactgctggg ttatttcatt attaatttca ttaagaactc ctgaaagcga
    13681 accatagtat ttatccataa tttgtgacga tgaattaaac acggggttaa attgatcgat
    13741 ggttgctgtc aactgactat ggtgtaacga ttcgcggcgt ccccacagtg tcattgtcaa
    13801 cgacgtacca actgatcctg acaaggtgcg aaaaaaatta ctcatactcg aggcattggc
    13861 aaatttatta tctggcaagc ctgaaaacga aatcgttgtt aagggtaaaa agaaacaggc
    13921 aacggcgaat ccctgaaaaa actgtggcaa aatgatgcct gtaaaatcaa tcgttggcat
    13981 aaatgtcaca gaacgccagt aatagcaaac cgcatacatc aaaaaactaa atgtcaccaa
    14041 caaccgcata tcaattttgt tgccataacg tcctatcaaa ggtgatatta atagcggcat
    14101 gataccgatg ggcgcatagg caagtccggc ccatatcgca ttatacccca tcgtttcctg
    14161 gagtaactgc ggcataagaa ctattgcccc agagtaaaat aaatacgcgc atgtgatact
    14221 cacaatacca atggtgaaat tacgggactt aaacaaactg aggtcaagaa tcggattctc
    14281 tgaggtcgac tcccaaatga ctaaagaaat cagagaaata actgatacta ctgttagtat
    14341 tattattgta ctcgagttga accaatctag atcgcgccct ttatcaagca taatttgcaa
    14401 accaccaaca ccgagcacta acagggtcag tcctggtaga ttcattttga ccggtgaagt
    14461 ctcagtttct cgtcctttaa gtaaggttaa gcataatgtc aggacgataa tccccatagg
    14521 gacattgatt aaaaatatcc aaccccagct aaagttatcg caaatatagc cgcccaatat
    14581 tggcccacat atcggagcga taattacggt cattgaccat aatgccaaag caaatgttct
    14641 tttttctggc ggataattcc ttaataataa actctgtgac agtggaatta acggccccgc
    14701 cactaagccc tgaacgactc taaaaaatat cagcacatca agattggtcg ataggctaca
    14761 cattaatgaa gatagtgaaa aaaaggttac tgaaagtaaa aataatctta attcgcctat
    14821 tctttgtgcc agcctgccgg taacagggat cgcaatggca tttgctacgc caaacgaagt
    14881 gataacccag gtgccttcgt ctgttgatgc tcccagaaag ccagatattg tcggtattgc
    14941 gacgttagaa atagtggaat ccaacatttg cataaacgtc gctaaggaca atgcaatagt
    15001 aacgcaccat aacgtcccac cggttaatgg tgccggagtt gatttagtga ttgccattaa
    15061 agttgtccat tatgcgaaat aatgttgcta atttcttttt ctatcggagt agtatcgata
    15121 actaaagcct tactggtata agcaggcatg gaggtcacgg ttgaagccag ctcaggcatc
    15181 tcagcaatgt cttcgtcctt cgtatcaata gttgcagtca tcgataaacc aatacgtaag
    15241 ggatgttcca tgagttcttt tggatccaga gacacctcaa ccggtacacg ctgaacgatt
    15301 ttgatccagt tccctgtcgc attttgtgca ggtaataagg agaacgcatt gccggttccc
    15361 atattgattc ctgtcacccg accatgaaac acaacatttt caccataaag atcgctgata
    15421 atattgaccg attgaccaat ccgtacatcc gtgagttgtg tttctttaaa gttggcatta
    15481 acccacattt gacgtgccgg tacgacagcc attaacgatt gtccgggact cactgtttcg
    15541 ccgacctgaa cacttcgctg ggcaatatag ccggtaacag gactcttaat atccgtacgt
    15601 ttaagcgcta accaggcttc tttggttgca tccgccgctt caacgacttg tggctgacgg
    15661 tttaatggtg tgttcattac taaagcttta ttcgctttat aagcctggat agcggcattc
    15721 aatgccgctt tgctacttat taacgtatct ttggtatgct ccagcgtttc ttttgaaata
    15781 accccctgct tcgctaaagg cacacgacgg ttataatctt ctaaagattg ttgatactga
    15841 atacgtgctg aagcgacttc ggcactgtat tgtttatcct gtaagtacag tttattcgtt
    15901 tgccgaacaa tatttgccag attattttta gctttattga gtgcgatagt ggcatcagtc
    15961 ttatccagtg agactaaaat gtcaccctgt cgaacgtagt tcgtatcttt atgattaacg
    16021 acagtgacgc tgcctgaaac ttgtgcagaa attggatctg cattccccgt gacataggcg
    16081 tcatctgtac taatcatgtc ttctaattcc attgaccaat aggcataggc acctgaaaac
    16141 gcaataaata aaactaccgc caataaagaa aagtattttc ttctgttaga atatttttta
    16201 tttgaattaa tctgttccac ttttatctct catttcgcat agatga
//
LOCUS       DAPHCV010000105        16033 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.105, whole genome shotgun sequence.
ACCESSION   DAPHCV010000105 DAPHCV010000000
VERSION     DAPHCV010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.105"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            82..1524
                     /locus_tag="RH033_003620"
     CDS             82..1524
                     /locus_tag="RH033_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004100781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HDV2992748.1"
                     /translation="MQVDWLIKNVTVIDGSGGPEFRGDVAITGDRIVDIAPALNVTAQ
                     QVIDGEGRVLAPGFIDVHTHDDINVIRIPEYLPKISQGITTVIVGNCGISAASAKMKG
                     EVPDPMNLLGEAEHFIYPTVESYAQAVEAARPSLNVGTLIGHTALRNNHMDDLFRPAT
                     VDEIAAMRADLRLALSQGALGLSSGLAYATAFQATTEEVMALAEELAGEKGVYTTHLR
                     SEFEPILDALDEAFRIGRHGKVPVVVSHHKCAGAKNWGRTKETLAFFDQMRQHQEIGC
                     DVYPYSASSSTLDLKQVTDEFDIVITWSQTHPQQAGKTLAQIAIDWQMSMLEAAKLLM
                     PAGAIYYNMDERDVRRVLSYPVSMIGSDGLPNDPMPHPRLWGAFPRVLGHYCRDEGLF
                     PLTTAIHKMTGLSASRFRLPQRGLVKVGYFADLVLFDPQTIRDVASFSDPKRPADGIE
                     AVMVNGVMSYGPDKHITGRAGRFLRRQTSH"
     gene            1534..1923
                     /locus_tag="RH033_003621"
     CDS             1534..1923
                     /locus_tag="RH033_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDV2992749.1"
                     /translation="MSIKRYGTEGGTGTGGQHLPFARAVEAGGWLYVSGQTPMKNGEV
                     VEGGIVEQSRLAIQNCVDIMTEAGYSLADVVHVKVILTDARYFQSFNKVFREFFGENP
                     PARICCVADLVVDCKVEVDVTCYNATR"
     gene            2026..3495
                     /locus_tag="RH033_003622"
     CDS             2026..3495
                     /locus_tag="RH033_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HDV2992750.1"
                     /translation="MNSHIFLVGFIIYAIAMIWLGWFVSRNQKSGEDFLLGGRSLPLF
                     LTLGSTVATMVGTGSSMGAVGFGYSNGWAGMLYGVGGAVGILLVAWLFAPVRKLRFMT
                     MSEELSYYTGGSHLIKNIVGLMIFIASIGWLGAHILGGSMYLAWATGIDLTVAKLIIA
                     LAFAIYVIIGGYSAVVWTDTIQALILFFGFILMAILAVVHVGGWSAIEQAMDPKAMSL
                     FAIDKMGVLPALSLALVIGVGVLATPSYRQRIYSGKDVPSVRRSFVITGVLYLFFSIL
                     PAIIGMAAFTMNPNLENSNYAFLFATSFLPAILGLVVLIAGLSATMSSASSDAIAAVA
                     IMMRDVYTMITGRMPPEDKAITYSRWMLTFVIGLALVFALTSNDIISYITKMISMLMS
                     GLFICSILGRFWLRFNWQGALAALVSGMVMSIVVLMNADWLAYWGNPCIPSVLGSLVA
                     GVLVTLVTPASQVSREEALAIITNERENQSVVITKPEEA"
     gene            complement(3505..4368)
                     /locus_tag="RH033_003623"
     CDS             complement(3505..4368)
                     /locus_tag="RH033_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDV2992751.1"
                     /translation="MAYSIDIISCITERFMALTATEKRIAQFILDDVSAAVELPIAEL
                     ARLTQTSQASVTRFARALGCKDVRELKVKLAQSLAVGQRFILDVPDLDGVQGIYEAII
                     SVLETHRRSLDMNAISQAVSWLSQARQILALGTGGGSTICSQEIQYRLFRLGLPVVSQ
                     NDALMMRMMCSAVMPQDVVIVLSLGGYTPEIIESAAIARQYGARVIAITPAQTPLAEQ
                     VDLVLPLLVQESDYIFKPTPSRYAMLAMVDVLATELAMANKAQAKDRLRRIKLVLDSH
                     RGGANRQPLGD"
     gene            4550..6214
                     /gene="tsr"
                     /locus_tag="RH033_003624"
     CDS             4550..6214
                     /gene="tsr"
                     /locus_tag="RH033_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HDV2992752.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQHQRETSAVVKTVTPAAPRKMAVADSGENWETF"
     gene            complement(6263..7624)
                     /locus_tag="RH033_003625"
                     /pseudo
     CDS             complement(6263..7624)
                     /locus_tag="RH033_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(7839..8753)
                     /locus_tag="RH033_003626"
     CDS             complement(7839..8753)
                     /locus_tag="RH033_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDV2992753.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPEETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8892..9914
                     /gene="lgoD"
                     /locus_tag="RH033_003627"
     CDS             8892..9914
                     /gene="lgoD"
                     /locus_tag="RH033_003627"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HDV2992754.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVENILPADGIDPQAAALIEPFAISAHAVRRAAM
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            complement(10054..12345)
                     /gene="opgB"
                     /locus_tag="RH033_003628"
     CDS             complement(10054..12345)
                     /gene="opgB"
                     /locus_tag="RH033_003628"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HDV2992755.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLAAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIHLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKAIWKGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(12599..13093)
                     /locus_tag="RH033_003629"
     CDS             complement(12599..13093)
                     /locus_tag="RH033_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HDV2992756.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSNGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            complement(13142..13879)
                     /gene="dnaC"
                     /locus_tag="RH033_003630"
     CDS             complement(13142..13879)
                     /gene="dnaC"
                     /locus_tag="RH033_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HDV2992757.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            complement(13882..14421)
                     /gene="dnaT"
                     /locus_tag="RH033_003631"
     CDS             complement(13882..14421)
                     /gene="dnaT"
                     /locus_tag="RH033_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HDV2992758.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     ITPARLAELLALEEKLARPGSDVALDDQLYQEPQTAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTAEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            complement(14529..15002)
                     /locus_tag="RH033_003632"
     CDS             complement(14529..15002)
                     /locus_tag="RH033_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HDV2992759.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            complement(14993..15763)
                     /locus_tag="RH033_003633"
     CDS             complement(14993..15763)
                     /locus_tag="RH033_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HDV2992760.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFLASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
BASE COUNT         3895 a         3900 c         4263 g         3975 t
ORIGIN      
        1 ctgggttttg cgtagttttc cactgttttt cgtccttgta aatggttaac ttgtcaccat
       61 tgcagaaatg gagtgacgaa catgcaggtt gactggttaa tcaaaaatgt gaccgttatc
      121 gacggtagcg gcggccctga atttcgcggt gacgtggcaa taacaggtga tcggattgtc
      181 gatattgccc ctgcgcttaa cgttacggcg cagcaggtca ttgacgggga agggcgggtg
      241 ctggcacccg ggtttatcga cgttcatacc catgacgata tcaacgttat tcgcatcccg
      301 gaatatctgc caaagatcag ccaggggatc actacggtga ttgtcggtaa ctgcgggatt
      361 agtgcggcgt cggcgaaaat gaaaggtgaa gttcctgacc cgatgaatct gttgggggaa
      421 gcagagcact ttatttatcc taccgttgaa agctatgccc aggcagtgga agcagccagg
      481 ccgtcactga acgttggcac gctgattggt catacggcgc tgcgtaataa ccatatggac
      541 gacctgtttc gcccggcgac ggtggatgaa attgccgcta tgcgtgccga cttacgtctg
      601 gcgttgagtc agggggcgct ggggttaagt tccgggctgg cctatgcaac ggcatttcag
      661 gcgactaccg aagaagtcat ggcgctggcg gaagaattag ccggtgagaa gggcgtttat
      721 accacccatc tgcgttctga atttgaaccg attctcgatg ccctggatga ggcgtttcgt
      781 attgggcgtc atggcaaagt gcctgtcgtg gtttcccacc ataaatgcgc cggggcgaaa
      841 aactgggggc ggacaaaaga aacgttggcc ttttttgacc aaatgcgtca acaccaggaa
      901 attggctgcg atgtctaccc ttactcagcc agctcttcaa cgctggatct caaacaagtc
      961 accgacgaat tcgatattgt gatcacctgg tcacaaacac atccgcagca ggccgggaaa
     1021 acactggcgc aaatcgccat cgactggcag atgagtatgc tggaggccgc gaagctgcta
     1081 atgcctgccg gggctatcta ttacaacatg gacgagcgcg acgtccgccg agtgttgagt
     1141 tatccggtca gtatgattgg ctctgacggc ctgcccaatg atcccatgcc gcatccacgt
     1201 ttatggggcg ccttccctcg cgtgctgggc cactattgcc gtgatgaagg cttattccca
     1261 ctgaccacgg ctatccacaa aatgaccggg ctttctgcca gccgttttcg cctgccccag
     1321 cgtggactgg tgaaagtcgg ttattttgcg gacctggtgt tgttcgatcc acaaaccatt
     1381 cgtgatgttg ccagcttttc tgatcccaaa cgcccggcag atggcattga ggctgtgatg
     1441 gtgaacggcg ttatgagcta tggtcccgat aaacatatta caggccgtgc gggacgcttc
     1501 ctgcgccggc agacctcaca ttaaggagtg aatatgagca ttaaacgtta cggtacggaa
     1561 ggtggaacag gcactggcgg gcagcatttg ccttttgcca gggcggttga ggcgggcggc
     1621 tggctgtacg tctccgggca aacacccatg aaaaacggtg aagtggtaga aggtggaatc
     1681 gttgaacagt cacgactggc gatccaaaac tgtgtcgaca tcatgactga agccggttat
     1741 agcctggcgg atgtggttca cgtgaaagtg atcctgaccg acgcccgcta tttccagtca
     1801 tttaataaag tattcagaga attttttggc gagaatccgc ctgcacgtat ttgttgtgtg
     1861 gctgatctgg ttgtcgattg taaagtcgaa gtcgatgtga cctgttataa cgcgacccgc
     1921 taataaattt aagaattaaa aaaaaagacc gcaattgagt gctgaataca ttcagcactc
     1981 cgggaactct tacattaatt tctctctact ggcaggatgt gaattatgaa cagtcatatc
     2041 tttttagtcg gctttattat ttatgccatc gccatgattt ggcttggttg gtttgtttct
     2101 cgtaatcaga aaagcggaga agattttctg ctgggcgggc gttcgctgcc gctgtttctg
     2161 acgctggggt caaccgttgc cacgatggtt ggcacgggtt ccagtatggg ggcagtgggc
     2221 tttggctaca gcaatggctg ggccggaatg ctttatggcg tcggtggagc cgttggtata
     2281 ttgctggtgg cgtggctgtt tgcaccggta cgtaaattac gttttatgac catgagtgaa
     2341 gaactctctt attatactgg cggtagtcat ttaattaaaa atattgtcgg cctgatgata
     2401 tttattgcct ctattggctg gctgggggcg catatattag gtggaagtat gtatcttgcc
     2461 tgggcaacag gtattgatct tacggtggca aaattaatta ttgccttagc ttttgcgatt
     2521 tacgttatta tcggcggtta ttcggcggta gtgtggacag atactattca ggccctaatt
     2581 ctgttctttg gctttatatt aatggctatt cttgccgttg ttcacgttgg cggctggagt
     2641 gcgattgagc aggcgatgga cccaaaagcg atgagcctgt ttgcgattga taaaatgggc
     2701 gtgctgcctg cgctttcgct ggcactggtt attggcgtag gtgtgctggc aacaccgtct
     2761 tatcgccagc gtatttactc gggtaaagat gttccctctg tacgccgttc gtttgttatt
     2821 actggggtgt tatatctgtt cttctcgatt ttgcctgcca ttatcggcat ggcggcgttc
     2881 accatgaacc caaatctgga aaacagcaac tacgccttct tgttcgcgac cagtttctta
     2941 cctgccattc ttgggctggt ggtgctgatt gccgggcttt cagccaccat gtcttctgcc
     3001 agctccgatg ccatcgcggc ggtggcgatt atgatgcgcg acgtctacac catgattacc
     3061 ggcagaatgc cgccggagga caaggccatt acttactcac gctggatgtt gacctttgtt
     3121 attggcctgg cgctggtctt tgctctgacc tctaatgaca tcatcagcta cattaccaaa
     3181 atgatttcga tgctgatgtc cgggctgttt atctgctcga ttctcggtcg cttctggcta
     3241 cgctttaact ggcagggcgc gctggcggcg ttagtgagcg gtatggtgat gtcaattgtg
     3301 gtgctgatga atgccgactg gctggcttac tggggtaacc cgtgtattcc gtcagtgctg
     3361 ggcagcctgg tcgctggcgt actggtgacg ttggtaacgc cagcaagtca ggtcagccgg
     3421 gaagaagctt tggctattat caccaatgag cgtgaaaacc agagtgtcgt gatcaccaaa
     3481 cctgaagaag cataaccgac aatgctaatc gccaagtggc tggcggttag cgcccccacg
     3541 gtggctgtct aacacgagtt ttatccgccg caggcgatct ttcgcctgcg ctttatttgc
     3601 cattgccagt tctgttgcca gtacatcgac catggctaac atcgcgtagc gggaaggggt
     3661 gggcttaaag atgtaatcac tttcctgaac cagtaacggc aataccagat ccacctgttc
     3721 cgccagcggt gtttgtgctg gcgtaatggc aataacacgc gcgccatact gacgggcgat
     3781 tgccgcactt tcgatgattt ccggtgtata accgcccagc gaaagcacaa tcaccacatc
     3841 ctgcggcatg actgccgagc acatcattct catcatcagg gcatcattct gactgaccac
     3901 cggtagcccc agacggaata aacggtactg aatctcctgt gaacagatag tggaaccgcc
     3961 gccagttcct aacgcaagaa tttgccgcgc ctgacttaac cagcttacag cctggctgat
     4021 ggcattcata tccagcgaac gacgatgcgt ctccagcacg ctaataatcg cttcgtagat
     4081 cccctgaaca ccgtcaagat ccggtacatc gagaataaac cgctgcccga cagccagtga
     4141 ctgcgccagt ttgactttca actcgcggac atctttgcac cccagtgcac gggcaaaacg
     4201 cgtcaccgac gcctgactgg tttgcgttaa ccgtgccagc tcagcaattg gcaactctac
     4261 tgcggcagag acgtcatcaa gaataaattg tgcgatacgc ttttcagtgg ctgttaacgc
     4321 cataaaccgt tcggtaatgc aggagatgat atcgatgcta taagccatag gagtatcact
     4381 ggttcgaaga agattgatac tttgtaccat aaactgccgc tatagagtgt gaccctcaca
     4441 aaaaacgcaa gtttgctgca acaaaagcag tgccgacgct tttccacagc ataaagtttt
     4501 ccaccaccag gccgacaacc agtgtatctg tctagtggaa agagaaacca tgttaaaacg
     4561 tatcaagatc gtgaccagct tactgctggt tttggccgtt tttggccttt tacaactgac
     4621 atcaggcggt ctgttcttta atgccttaaa gaatgacaaa gaaaatttca ctgttttaca
     4681 aaccattcgc cagcagcaat ccacgctgaa tggcagctgg gtcgcgttgt tgcagacgcg
     4741 taacaccctc aaccgcgcgg gtatccgcta catgatggat cagaataata ttggtagcgg
     4801 ttcaaccgtt gctgagctga tgcagagtgc cagtatttcg ctgaaacagg cggaaaaaaa
     4861 ctgggcggat tacgaagcgt tgccgcgtga cccgcgtcag agcaccgccg cagcggcaga
     4921 gatcaaacgt aattacgata tttatcacaa tgcgctggcg gagctgatcc aactgttagg
     4981 tgcaggcaaa atcaacgagt tctttgatca gccgacccag ggatatcagg acggtttcga
     5041 gaagcagtat gtggcttaca tggagcaaaa cgatcggctc tatgatatcg ccgtcagcga
     5101 taataatgcc tcctacagcc aggcgatgtg gattctggtg ggcgtgatga tcgtcgtact
     5161 ggcggtcatc ttcgccgtct ggttcggtat taaagcctcg ttggtagcgc caatgaatcg
     5221 cctgattgac agcattcgtc atattgcagg cggcgatctg gtgaaaccga ttgaggtgga
     5281 tggctctaat gagatggggc aactggctga gagtttgcgc catatgcagg gagagctgat
     5341 gcgtaccgtc ggtgatgtgc gcaacggggc caatgccatc tatagcggtg ccagcgaaat
     5401 cgctaccggc aataacgatc tctcttcgcg caccgagcaa caggccgctt cgctggaaga
     5461 gacggcagcc agcatggagc aactgaccgc aacggtgaag cagaacgccg aaaatgcgcg
     5521 ccaggccagc cacctggcgt taagtgcttc tgaaacggcg caacgcggcg gcaaagtggt
     5581 agataacgtg gtgcagacta tgcgcgatat ctccaccagt tcgcagaaaa tcgccgatat
     5641 tatcagcgta attgacggca ttgccttcca gaccaatatt ctcgctttga acgcggcggt
     5701 tgaagcagcg cgcgcgggtg agcaagggcg cggttttgcg gtggtcgcgg gtgaagtgcg
     5761 taatctggcc cagcgcagcg ctcaggcggc tcgtgaaatt aaaagcctga ttgaagactc
     5821 ggtggggaaa gtagatgttg gctctacgct ggtcgaaagc gccggggaaa ccatggcaga
     5881 gattgtcagt gccgtgaccc gcgtgacgga cattatgggc gaaattgctt ctgcttctga
     5941 tgagcagagc cgtggtatcg atcaggttgg attagcggtt gctgagatgg accgggtaac
     6001 tcaacagaac gccgcgctgg tggaagaatc tgccgccgcc gctgccgcgc tggaagagca
     6061 ggccagtcgc ctgaccgaag ctgtggcagt gttccggatt cagcaacagc agcaacatca
     6121 gcgtgaaaca tcggctgtgg taaaaaccgt gacgccagct gcgccgcgta aaatggccgt
     6181 ggcagatagc ggggagaact gggaaacgtt ttaatcgcca taaaaatgcc cgataagcaa
     6241 aatgttatcg ggcataggga gattaatctt tacgcgggtc gttgatcggc tgacgaacca
     6301 ggaagatgta cgccagtgca cccgccgcgg tgacgcaacc gcagatgatt agtgccagac
     6361 ggaatgagtg ggtggtatca acaataaaac cagtaatgat cggcgcaaaa gaggcgcaga
     6421 tgaagctggc aaagttctgg atactgccca ccgacgccgt catgcgagaa gcaactgcga
     6481 cgtggatcaa gccccagcag gatgttccgg caaagtgaat acagaacagt gccatgccaa
     6541 tcagcagaac cgccgtcatg gatgttgtcg cttgcggtac gaccagcgta aaggcggcag
     6601 aacagaacat cccggcaata atgcagatct tacggctttt aatcggagcc attccccctt
     6661 tgaccagtca gtcagtaacg taaccgttga ccagcatccc ggcagcccca aacaggaaag
     6721 ggatagccgc catcaaccct gtgcttttta aatccaggtt ataggctgtt tgcaggtaac
     6781 caggaagcca ggccagatac agccacgcag tgtagttgat gccactgaat ccgagcatca
     6841 ttccccacat tgtgcggtta cggaacaagc tgcgccattc ggcaaaactg agcggatctc
     6901 ggcgggcatt gacgctacct gcattgagat aagcttgttc aacggcagtc agttctacgt
     6961 gctcgcggtt gcgatagagc atataccagc cgatggcgag aaaaatcccc agtacaccaa
     7021 tagtaataaa catcccgcgc cagcccatca ccagcatcat cgccgccaga atcggtgggc
     7081 taacggcaac gccaatggta gaagctgcgt tgaagaaacc catcgggcgt ccgcgctctt
     7141 tgatgttgaa ccagtcgtta atgactttta cgccacatgg gttcatcggc gcttcaccaa
     7201 tccccatacc gatacgcacc aacacgaact gcgtaaagct gtgaaccatg ccagacatgg
     7261 cctggaacag tgaccagaag aacatcccca gtcccagcat caggcgcggg cctttacgat
     7321 ccaatagtgg gccgcaagga agttgcgcaa tcccgtaagc gagtgaaaac acggagagca
     7381 aagcgccgat ttcggtggca cttaatccca attcttcacg aatcgttaaa tttgctaccg
     7441 acagcgaact gcggtcgaga taattgatta ccgccgcaaa aaataataac aacatggcgg
     7501 tggtttgaat gcgtttaatt cgggtactgc gttgaactaa tccatctggt ggcacgggaa
     7561 tatctgccga cgaagatgga gtaaatgaag aacgcggatc gagggtgata ttttcttttt
     7621 ccacgccgga ctcctgtatt tataatttta attgtttacg taattgtgca ctacgcaagg
     7681 ttgacattaa cggcaggtta aagttttagg actttactac cgctcacaaa aacattttta
     7741 gtcgactgat tgtgagcata ggtaagcggg aatttcccgg tatttatctt ttgataaaac
     7801 cgtgcacccg ctaatagttt gctgttttta atccgtactt aatgagcata acgcgtgttc
     7861 tcattaatgg atcgaatcat tgattgttta gctgaattca aatggttacg gagcgcaagg
     7921 gtggcatcaa ggtcactgcg gcaaatcaga gcgctgagaa tggtcatatg ctcatcgacg
     7981 gcaatgatat tgcgctgttt aagatcgctt tcgtcccatt gatagtgaaa atgaaagatg
     8041 actgagatga tctcaagcga ttgatcaaaa aagatattgt cggcagctga aagcagtaag
     8101 gagtgaaaat ctctgtcaag ttgcgagaac atgcgaaagc tattgccgat gttgtcacgc
     8161 agtagccgat gacgttcgag catggttttc gcctgcagcc agcggggatc atgatcaggc
     8221 aggttgagaa agtgctgaag tgaatgagtt tcgagcattt cgcgtagctc aaacagctgc
     8281 tcagcgtagg attgatcgaa ttgcttcatg ctccactggc cgcgcttttc gctttgaata
     8341 agattataac ggccgaattt taaaagatat tctcttacta caaccgggct gacacccgcg
     8401 gctcgagcca gttgcagttc agaaaaagtt tcctctgggc gtaactggcg ctggtttatc
     8461 atcgtgaaaa aagcctgctc aaacactttg ttttgttcac tcattgaagc ggtagtacag
     8521 gcgaaaccgt catcatggtc aggtttacgc gcaataacat agtcgtttcc aacctgcgtt
     8581 aagacgccgc attcgcgtaa gtggctgaga atatgacgca ctgtagtgcg gctgatgttg
     8641 tacatttccg ccagcgcact ttgcgatgga agtggggaag ggatatgacc ccgcgccata
     8701 tcatcaatga cctggttaat cacattgtgg cgtaaatttt gtgaacgact catattttcc
     8761 tccttgtgag tccattaaaa cccgatttaa aacattttta tgcgtgtgtt ttcacaaaac
     8821 agattttccg ttccgctgcc atttttcatt ttcattgata tctaaggcaa caaaaaatgg
     8881 agagccgaat catgtctacg atgaatgttt taatttgcca gcagccgaaa gaattagtct
     8941 ggaaacaacg cgagatacct attccgggtg acaatgaagc attaataaaa attaagtctg
     9001 tcgggatttg cggtaccgat attcatgcct ggggtggaaa tcaaccattt tttagttatc
     9061 cacgggtttt aggccatgaa atatgtgggg agattgttgg gctgggtaaa aatattgctg
     9121 atcttaaaaa tggtcagcaa gttgctgtga tcccttatgt tgcctgtcag cagtgccctg
     9181 catgtaaaag cggtcgtacc aattgctgtg aaaaaatttc agttattggc gtgcatcagg
     9241 atggtggttt tagtgagtat ttgagcgtgc cggtggagaa cattttgccc gcagacggta
     9301 ttgacccgca ggcggcagca ttgattgaac ctttcgctat tagcgctcat gcggtgcgtc
     9361 gcgcagccat ggctcccggc gagcaggtgc tggtggtcgg ggcggggcca atcggtctgg
     9421 gcgcggcggc aatcgctaaa gccgatggcg cacaggtagt ggtggcggat accagtccgg
     9481 cgcgccgtga acatgtggca acgcgtctgg agttgcccgt gctggaccca tcagccgaag
     9541 attttgacgc gcagctgcgg gcgcagtttg gtggttcgct ggcgcagaaa gtgatcgacg
     9601 cgacaggtaa tcaacatgcg atgaataaca ccgtaaatct gattcgtcac ggcggcacgg
     9661 tggtatttgt cggtctgttt aaaggtgagt tgcagttctc cgatccggaa ttccataaaa
     9721 aagaaacgac gatgatgggt agccgcaacg ccacgccgga agatttcgct aaagtcggtc
     9781 gactgatggc ggaaggaaaa atcactgccg acatgatgtt aacccatcgc tacccgttcg
     9841 ccacgctggc agaaacctac gagcgcgatg tgattaacaa tcgtgagttg attaagggcg
     9901 taattacttt ctgataaggc gagattatta aagttgccat gcagcgtccg gggaagtgtt
     9961 gggcgctgtt ttttttctgt ttctcaataa tgttttgtag gccggagaag gtgtgtaggt
    10021 cgcactttat gcgacctaca catcagtcag gaattaccct tcacgttcta ccactttaat
    10081 ttccaccatg ccgatcccga gcttacgcgg cgagtgcccg aggatattcc cttcgttggt
    10141 tgagacaggt tccggtggca caattaccag tgtgtcggca tcggttgggt tatcgaaatg
    10201 cagtgtggtg gtggtcactt cattgcccag cacaagggtt tgttcttcat tgcctacgcg
    10261 taccggaata ggacggctgg cgttattgcc gtatgctttg gcggtaatca ccaggtcaaa
    10321 tttcttcggc agcggatgct tgtactcgat ttttacttca tcgcccagct gtgcgttgga
    10381 ccagcggccc cacgactctg gacgagaaat cccgctaaac tgtttcacct cttctggcgc
    10441 accagcgacg ttaaagatga aactgtcggc tttgtagcga atatcgttat caacaatctt
    10501 tagcgtatcg acattgcctt tgtaacgcgc catgtcgatg accgtatctt taaatgccgt
    10561 tttgcccttc catattgctt tgtcaacatg ctgaacaatt tgctgaccgc caagctgccc
    10621 ttgcgagaca caccagtcgg tggaaagtgc cagttccgga gcccagagtt gtgccatctt
    10681 atagcaacgg tcaacccaga cgaaattgtc gcgtggagcg aaatcagcca gctggaaacg
    10741 cagcggtgct gagtattcgc tttccggcag cggttccacg cgtttgtctg aaacccgcaa
    10801 cagcagcggc aaacggaaat ggctacctga gaaggcaatc atgtttttct gctggtcgat
    10861 ggtgaactct ttcatctctt tagggaattt ccacaggtgg atgatatccg gcttccacgc
    10921 caatgttttc tctttgatat tgaggaagat ttccgacatc gactgcccgg ataaactgct
    10981 acggccaagt ccgagatagt tatcgccacc gagaatgtcc agcaccgtcg cgccgttatc
    11041 catcgtatta cgcttcactg ccagcgtctc ttgctgcggc ttgtcgccac ggatgacaaa
    11101 aaacaggtta ttgcgatcct gcttattaag gtatttccac gccgtattgt tcatcgctaa
    11161 atggtcagaa gagacgacga tgacggtatc tttaaaccac ggtgacgctt tgattttgtt
    11221 gataaacgtc gcaatattct cctggctgca acttaccgcg ctgaatgact gattcggctt
    11281 accgtcaaaa tcatattttt tgcggttaca ggtgcgcgag ataaaaccat ccgggtgatg
    11341 ggtatcgact gtcagggtaa acagtgagaa tcgctgacct gagcgggaaa gctcttcaaa
    11401 ttttttccac gcttcatcga gaacggtatc gtcgtagaat ccccagtcgt tgcgatagtg
    11461 cgggtcggcc accacgcttt tcagctcttc tgagccgtat aagtggtcga agccgtgcga
    11521 tttcaggaac acatctttac cggcaaaacg cagattcgcg ccctgcacga aatagttctg
    11581 ataacccgag tttttcagaa tatcgcccag acagatgttc tgcgggaaga agctggaaac
    11641 agaagcggag gcgttgcctt caaatggggc aaacagcggt atgccgcact gagaagccac
    11701 catgcccgca atcgtgtaat ccgttcctgg cagctgctgc gtgtggctga aatccaggcc
    11761 ttcatttttc aacgcgccca gttcaggcgt gagatccggg aaagcctcgt tatcaaaata
    11821 ggtccgctcg agactttcgc cgtagatata taccaggttg agtttcgggt cagggatagt
    11881 tttcgacggc tctttataat aagccgcaaa gtccgggtcg ccgtcgcgtg actgggattt
    11941 caccagttcc gttatctgac gaaatgccgg gctggcatcc actgaaccca gcgccagtaa
    12001 gagtgccagc aggctgtaac caaaatggtg cggatgatga cgacgacggc gcaggatcca
    12061 gcccagcgca ccgaacaccg ccgccagccc cagcacaatg ccgataccag gcaggatgta
    12121 tttgctgaca ccagcaccgg tcaggctgtt ggttaaggta tagagaaccg cgtcgttaat
    12181 accatcgcca gtaaaatagt cgctggcaaa cagggtgata tttaaaacga caaatagccc
    12241 cagtaccgtt aatgtggctg caaaccacca ggtgttacgt cccgctttcc atgcgtaaat
    12301 cagcacagag gcgagaaaaa gggcgaaaga gagtagttct gacaacgggc gatcctcact
    12361 aaaccgggcc gtttagccaa cgtttaagct ttttgagaag cacaatgtaa tggcgtcgcc
    12421 atattgctgc aattctagtg tcaaaaaaat gcgatgttgt taggaattag tttataaaca
    12481 gacttttttg atcgcggtcg cttcccgtgt aacaggggta aaaccgtcca ggagcaggga
    12541 aaagggcagg agagagccgc gtcagccggg taacagctaa aacgcggcgt ttcgcggttc
    12601 aggaaatgaa gttcagcccc tgctttaaca ccagatcgca ggctttggtt ttcacctttt
    12661 ctgccagcgg agtcgttcca atattatcca gattgagttg ctgaccatct ttggttttca
    12721 gcaaaccctg aatgccatcc agatagttgg tgtcttcttt ttgctcttca ctgttcaggc
    12781 ccagtttttc cagtacctga ttcttgatgt tttcggcatc ggttaccgaa gccagctttt
    12841 gcttcgcgca gtattgcaga atgcctgcgg cgttgttcat gttatctgcg cttaaggcct
    12901 ggttcccgtt gctaagcaag ttagttaatg acgcgagcga ccaaccgcct tcctgtgtcg
    12961 tgctgttttg gttgccaagt tcgctggcgg cgctggagag cgcatctttc caggacgcag
    13021 catgcacccc ggtggaaatt aatgcgctgg cggcaatggc acagcacaga agatgtttaa
    13081 tagttttcat catcattact caaggttgaa ttgtgtcgca gtataccgcc agaaacgggg
    13141 attaatactc tttacctgtt acacggctac ggtagctatc ccagttgaag atcacccaca
    13201 aactgttacc caggcgcata cggtccatca cgcgttcgcc cagcaacttg gtcatctctt
    13261 ccatattact gttggtcagc atcccggttg ggcgtttgga agaagagcgg cgatcgacga
    13321 tctggttgat gatcactttt tcgtatttcg attcggtctg cacgccgatc tcatcgatca
    13381 ccagcagatc aacgttactc agatcgttaa gcagttgttc ttcgctggta ccgctattcc
    13441 tgaaggtatc tttcatcgcc gacataatat cggccacggt gatgatcaat acggatttac
    13501 cgcgtagcag cagctcgttg cagattgccg ccgccagatg gtttttgccg gttcctggct
    13561 taccagaaaa gatgaagctg gcgatgttgc cgtcaaactc ttcgacatac tggcgcgctt
    13621 tgcttaacgc attcatttgc ccttcacact caacgcgata gttctcaaag gagcagttct
    13681 gatgcagtgg acgaataccg gagcggttaa aggtgcgctg cattttcatc gcccgatttt
    13741 cacgttcgag agcggcggag cggatcgccc cttgttcttt ctgccaggcc agtagttctt
    13801 cacccgtttt gaacgcaggt ttgatatggg caggcatcat tttttgcagg cgttgcatca
    13861 agtcgccaac gtttttcatc gttaccctct gaatcctggc ggaatttggc tgtcaggttc
    13921 actgaccgta ttcacatctc gtttcggcag tccgccgtta ctggcacgac cgatttgcag
    13981 gctgcgcgcc agtttttgtt gccactgcac atggtgaaag actttgcctt ccgcctgcca
    14041 gtaggcaatg aatgacgcca gttcttcggc ggtcaccggc tctcttagtg ccacgcccca
    14101 tagcgccgcc aggcggataa agtcggcatc gggttgccag tccggataca tggcaaattt
    14161 ccccatgggt acggcaacgg gagcggtttg cggttcctga tagagttgat cgtccagggc
    14221 cacgtcactt cccggacgcg ccagcttttc ttccagcgcc agcagttcag ccaggcgtgc
    14281 aggagtgatg gcataaaacg ccggagcatt gttagcaaat acggcaacca caccgccttc
    14341 agcttttgcc agaacggttt ggtgatcgtg taccagggcg tcaataccaa cgacgtccgg
    14401 ggtcaaaact ctggaagaca tactgtttct caaatatgga acgatgaacg agaagggaga
    14461 taacttttat agtaacacac ggaggagagg aggctgtctc tcatccgcgt gctttaagtg
    14521 aggcaatttt atacgcgagg gcgcttgcgg tacaaccata atccaggaat ggaaagaccg
    14581 atggataacg caccaacaat cgatgaagct gtaagaaagt tggttaacag ggtaatcatc
    14641 aacggttcgc tgtaacctag ctggctgatt tttaccgccg aaatcattgc ggtataagct
    14701 gatatgccag ggaacatagg gataacagcc gccacggtaa acactttcgg atgcgccaga
    14761 taccagcgcg accattgaat accaatggtg ccgaccagca tagaagccat aaaagttgac
    14821 cactcaatat tcaacccgct ggtcattaag atcattcggg aaccatgacc tatcgcacca
    14881 agcagcgcac accagcgtaa cgcccgcacg ggaacgttga acaccatcgc aaagccgacc
    14941 gcaggaatgg cggcgaggat catatcctgc gccaacgcta acagaaattc gatcacaccc
    15001 atccgcgtag cccccaaatc gtcagtgcca tcacgacgcc gacgcaggta gccagtgtca
    15061 gcagactggc gatcgcccag cgtgccagtc cggtattgat gtggccttta aacatatcgg
    15121 cgacggcatt aatcaacgga aaacccggca ccagcagcag aacgctggcg gccatcgcaa
    15181 tggtgggggt attgctgaaa gtgggaagtt gcaaaagcaa tccggaaatg gtggtggcgg
    15241 cgaaagcggt gaggcaaaag ttgatctgtg gatgaagatg acgttgtgcc agcagctggc
    15301 ggatatacat cgcggccgta ctggccagga aggtgatgac ggcaccatcc cagccacctt
    15361 tattgagttt acagaaacag gcgcaggaaa ggccaaccat taaggctacc agccatcttg
    15421 ggtaacgtaa tggctgaatt tggctaaatc gtttctctac gcctttgtaa tccagcagat
    15481 gatgctccgc cagaattaca atgtgctgga cttcagtcac cacatgcata ttaatgccgc
    15541 gatcgtgatt tttacgtgtc gatgtcaggc attgcccatc tttaatggtt gtcagcacga
    15601 tggcgttcga agagatagaa ctttcgacgc tgtccattcc caacgcccga cccagtcgtg
    15661 aggaaagctc atcaaccaac gcgctttccg caccatgttg caaaagaaat aatccacact
    15721 ggatacaaag ccgtgttacg gctcgctgtt gctcagtttg cataactcgc cctttgtcat
    15781 agacgtgctg cgtattgttc agttaatgag aatccatttg tatcatgaag cacggttgtt
    15841 ttgcggtagg ttaagatcaa aattgcgctg attaatcctg gttatcggga gatgctcggt
    15901 gataaaaagc gaattatctc tcgccgtaaa caaactatcg catgaatatt atcactataa
    15961 tgaatttgtt atttggcctt tacgagtcag gataatagat agctgggcgc gttttttaat
    16021 gttaacaagt taa
//
LOCUS       DAPHCV010000106        15888 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.106, whole genome shotgun sequence.
ACCESSION   DAPHCV010000106 DAPHCV010000000
VERSION     DAPHCV010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.106"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1350)
                     /gene="uidB"
                     /locus_tag="RH033_003634"
     CDS             complement(<1..1350)
                     /gene="uidB"
                     /locus_tag="RH033_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HDV2992761.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQAQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     "
     gene            complement(1347..3158)
                     /gene="uidA"
                     /locus_tag="RH033_003635"
     CDS             complement(1347..3158)
                     /gene="uidA"
                     /locus_tag="RH033_003635"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HDV2992762.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYLLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(3554..4144)
                     /gene="uidR"
                     /locus_tag="RH033_003636"
     CDS             complement(3554..4144)
                     /gene="uidR"
                     /locus_tag="RH033_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HDV2992763.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(4365..5132)
                     /gene="hdhA"
                     /locus_tag="RH033_003637"
     CDS             complement(4365..5132)
                     /gene="hdhA"
                     /locus_tag="RH033_003637"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HDV2992764.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFAWRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(5244..6272)
                     /gene="malI"
                     /locus_tag="RH033_003638"
     CDS             complement(5244..6272)
                     /gene="malI"
                     /locus_tag="RH033_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HDV2992765.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECSSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            6447..8039
                     /gene="malX"
                     /locus_tag="RH033_003639"
     CDS             6447..8039
                     /gene="malX"
                     /locus_tag="RH033_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HDV2992766.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            8049..9221
                     /gene="malY"
                     /locus_tag="RH033_003640"
     CDS             8049..9221
                     /gene="malY"
                     /locus_tag="RH033_003640"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HDV2992767.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGKQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDSYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNSALPELNWQIPQSTYLAWIDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            9325..10326
                     /gene="add"
                     /locus_tag="RH033_003641"
     CDS             9325..10326
                     /gene="add"
                     /locus_tag="RH033_003641"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDV2992768.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(10362..11402)
                     /locus_tag="RH033_003642"
     CDS             complement(10362..11402)
                     /locus_tag="RH033_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDV2992769.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELEALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            11645..11770
                     /gene="blr"
                     /locus_tag="RH033_003643"
     CDS             11645..11770
                     /gene="blr"
                     /locus_tag="RH033_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HDV2992770.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            12043..12258
                     /gene="ydgT"
                     /locus_tag="RH033_003644"
     CDS             12043..12258
                     /gene="ydgT"
                     /locus_tag="RH033_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HDV2992771.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            12344..12784
                     /locus_tag="RH033_003645"
     CDS             12344..12784
                     /locus_tag="RH033_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HDV2992772.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGEQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            12861..13442
                     /gene="rsxA"
                     /locus_tag="RH033_003646"
     CDS             12861..13442
                     /gene="rsxA"
                     /locus_tag="RH033_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDV2992773.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            13442..14020
                     /gene="rsxB"
                     /locus_tag="RH033_003647"
     CDS             13442..14020
                     /gene="rsxB"
                     /locus_tag="RH033_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HDV2992774.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            14013..>15888
                     /gene="rsxC"
                     /locus_tag="RH033_003648"
     CDS             14013..>15888
                     /gene="rsxC"
                     /locus_tag="RH033_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDV2992775.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQIPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQNCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKAAVE
                     A"
BASE COUNT         3778 a         4159 c         3965 g         3986 t
ORIGIN      
        1 ttgctgctga acttttttac gattatcaat ttcaacaacg atttctttga attttttatc
       61 cgtgagcgga taaaaccaga taatgacgaa tgccagtagc ataaatccgc aaggtactaa
      121 ggcaattgat gtgcggatgc ccataataac ttccggcgtt tgcgcctgat tggcgatata
      181 tccgcttaat cccaaaataa aggcaggaat tgaacctccg attgcctgac cgcatttacg
      241 ggtaaatgag aatagtgaat aggtgagccc ttcaattcgc acgccggtca ggtattcacc
      301 gtattctacg gtatcagctt ccagtgccca catcacggtc atggtaacgc cctggccaat
      361 tgatgcgatg gccaacgcaa ccaacgccac cggcagtgac cagacggaaa cccagaagaa
      421 cagcagataa ccgcaggttc ccagcaaagc gccaatcagg aaggtattct ttttaccgat
      481 cctcgcgacc atccccggca ccagcggtgc cgatgccaca gtaccgacca ggttttgcac
      541 cagtaccagc acagtgaaca gcccggtatc atttaacaca tagcgcacgt agaacaacga
      601 cgaggcgctg accgcgaagg tcgaaatcag cacacacagc gcaccgatgc acaacataaa
      661 cagcgggcga ttccgtttca gggtttgcag actgatcttc aatgacggct gcgccacgat
      721 acgtaccaca ttctcacgcg tcgatttgaa gcagatgaag taaagcacca ttccggcaat
      781 cgccagcaca attgtccaga aatgatatac cgacaccatc tcttccgggc tggagttctt
      841 aatgctcggt cctatcagaa atgccaggca gacaaaggtc aatgaagcgg caatcccgcg
      901 agccgcgccc agacgggcgc gggattgtgg ttgttgggtc atcgcggtag caagtgaacc
      961 ataaggaata ttcaccaggc tgtagcaaag cccgagtccc atgtaggtca aatatgcata
     1021 cacaactttg ctgctatggc tccagtcggt cggcacccag aataccagca cgctgaagat
     1081 cattaacggc gcagtaccga agagtaaaaa cgggcggaat tttccccatc gggtattcac
     1141 actgtccacc actcgtccgg caaagacatc ggcgaaggca tcgaataccc gcaccagtaa
     1201 cagcatggtg cccgccgcag cggcaccgac gccagcgacg tcggtgtagt aactcaacag
     1261 gaagagcgcc cccattgcga aggcgaagtt attggcgacg tcaccgaggc tgtagccgac
     1321 gatggtgcgc caggagagtt gttgattcat tgtttgcctc cctgctgcgg tttttcaccg
     1381 aagttcatgc cggtccagcg tttttgcagc agaaaagccg ccgacttcgg tttgcggtcg
     1441 cgggtgaaga tccctttctt gttaccgcca acgcgcaata tgccttgcga ggtcgcaaaa
     1501 tcggcgaaat tccatacctg ctcaccgacg acagcgctga cgcgatcaaa gacgcggtga
     1561 tacatatcca gccaggcgca ctgatactct tcactccaca tgtcggtgta cattgagtgc
     1621 aggccggcta acgtatccac gccgtattcg gtgatgataa tcggctgatg cagtttctcc
     1681 tgccaggcca gaagttcttt ttccagtacc ttctctgccg tttccaaatc gccgctttgg
     1741 acataccaac cgtaataacg gttcaggcac agcacatcaa agagatcgct gatggtatcg
     1801 gtgtgagcgt cgcagaacat tacattgacg caggtgatcg gacgcgtcgg atcgagttta
     1861 cgcgttgctt ccgccagtgg cgcgaaatat tcccgtgcac cttgcggacg ggtatccggt
     1921 tcgttggcaa tactccacat caccacgctt gggtggtttt tgtcacgcgc aatcagctct
     1981 ttaatcgcct gtaagtgcgc ctgctgggtt tccccgttga ctgcctcttc gctgtacagt
     2041 tctttcggct tgttgcccgc ttcgaaacca atgcctaaag agaggttaaa gccgacagct
     2101 gcagtttcat caatcaccac gatgccatgt tcatctgccc agtcgagcat ctcttcagcg
     2161 taagggtaat gcgaggtacg gtaggagttg gccccaatcc agtccattag tgcgtgatcg
     2221 tgcaccatca gcacgttatc gaatcctttg ccgcgcaaat ccgcatcttc atgacgacca
     2281 aagccagtaa agtagaacgg tttgtggttg atcaggaact gttcgccctt cactgccact
     2341 gaccggatgc cgacgcgcag caggtagata tcacactctg tctggctttt ggctgtgacg
     2401 cacagttcat agagataacc ttcacccggt tgccagaggt gcggattcac cacttgcaaa
     2461 gtcccgctgg taccttgtcc agttgcaacc acctgctgat ccgcatcacg cagttcaacg
     2521 ctgacatcac catttgctac cacctgccag tcaacagacg cgtggttaca gtcttgcgcg
     2581 acatgcgtca ccacggtgat atcgtccacc caggtgttcg gcgtggtgta gagcattacg
     2641 ctgcgatgga tcccggcata gttaaagaaa tcatggaagt aagactgctt tttcttgccg
     2701 ttttcatcgg taatcaccat tcccggcggg atagtctgcc agttcagttc attgttcaca
     2761 caaacggtga tacgtacact tttcccggca ataacatacg gcgtgacatc ggcttcaaat
     2821 ggcgtatagc cgccctgatg ctccatcact tcctgattat ttacccacac tttgccgtaa
     2881 tgagtgaccg catcgaaacg cagcacgata cgctggcctg cccaaccttt cggtataaag
     2941 acttcgcgct gataccagac gttgcccaca taattacgaa tatctgcatc ggcgaactga
     3001 tcgttaaaac tgcccggcac agcaattgcc cggctttctt gtaacgcgct ttcccaccaa
     3061 cgctgatcaa ttccacagtt ttcgcgatcc agactgaatg cccacaggcc gtcgagtttt
     3121 ttgatttcac gggttggggt ttctacagga cgtaacataa gggactcctc attaagataa
     3181 aaatactggt caacctttaa tctgattagt taaccaattc tgcgttgcac acagttatct
     3241 gtgagaaccc catggaagag aaatggaaat aaaaagaact gacaccggga tcaaaaaacc
     3301 aaaaactgca atacccacag cgtgatatag atcgcattaa tctttaaaac agcgttgaca
     3361 taagccataa aataagcaat cgaacgttcg ttcgaacgta ttgggaacga taaactctca
     3421 tgacatatga ctacggtgaa tattttgggg gggggaattc tccgctcccg gggaggatga
     3481 tttttgcaca gagaaattac gatatgaaac gcgggctcag ttgtcatcag cccgcggaaa
     3541 gagagagaga ggatcaggat gcggttaaga taccgccaat catcgcgcgt aatccctgag
     3601 caaacgacgc ttcacgtgcc agatcttccg cttcgatatc ggccagtaca ccgtaggtca
     3661 gatccagcag taaacgtgaa gtcattgccg tgttgatgtc tgggcttatc tcgccttttt
     3721 gctgggcatc acgcatccgc tgggcaacaa attccgtgat cgtcatatgc ttatttttaa
     3781 gcatggcggc gacctgtggg ttacgcatcc cttccgccat aatttcgacc accagcgccc
     3841 gttgtccaaa ggcttcatgg gtgagagata caatagactc gaccatatag tcaacgaaat
     3901 gaatcccttc aatcggttcc cggaaacggg ccagcgccct ctcctggtcc tgtaagataa
     3961 tcgcctgaat caaggcttct ttggagatga aatggtgata gagcgtccct ggactaatgg
     4021 cgcaagattt acagatggct ttcatcgagg cactgtgaaa tccattttct gaaaaaatct
     4081 ctctggcagc attgaggatc cgggtccgtg tcggttgtgc ttcagtctgc atgttatcca
     4141 tcataaaacc attatagtta gcgtgaataa ctagtttacc gcgttgtcag gtgagcgcct
     4201 atcattgctg ttgaatagtg cgaaggcaca ctctattcgt atacttttca tccagatatt
     4261 ggttaaccaa tagccgtgat ttggctgcca gttgtttagc agaaacgttg atgagtttat
     4321 cccggtaaac aacacgcacg gttgtttacc ggtccgttag tgtattaatt gagctcctgt
     4381 accccaccac cggagacggt gagaatttgt ccgcttaccc agctcgcagc tggcgagcaa
     4441 aggaacagcg ccgcgttagc aatatcttgc ggttggccca gacgtctgat cggcgtgtgc
     4501 tgtaacattt tttgttcaat ttctggtgta ataacggatt tcagggcatc ggttaatatt
     4561 gcccccggcg caatgccatt tacccgaata tttttttccc ccaggtcaaa cgccatattt
     4621 ctgaccagat gactggccgc agctttagat gatgcataag aagtcatgtt tatattttta
     4681 ttttctgccg ccatagaagt gatggtcaga ataacgccac cgccattttt ttccatttct
     4741 ggcgcaacaa gttgtgacag atggaaaaaa gaaaacacat tcagttcata agcacggcga
     4801 aaatctgcca ttggcatatc aaacggttta ggaccaccgc caccggcgtt gttaaccagg
     4861 atatcaactt tacccagctt actgacggca aagtctgcca gtgcagagag ttcctgttcg
     4921 gaagtaatat cacaacgcca ggcaaatgcc tgaccaccca gttgttgaat ttcatctaca
     4981 acatggttag ctgcgtcggc gttaatatca ctgaccacca cagatgcgcc agctgtcgcg
     5041 aatgtaatgg caatttcttt accaatacct gcaccggcac ctgtgatgat ggcacatttt
     5101 ccgtcgagtc tcaggttgtc agaattaaac acataaacct cctgtgaact tcagtaaaaa
     5161 taaaaggcac cgcctgtgtg ccacattttt tatcattaac cttgaggaaa ggacactacc
     5221 aggtatagca gataaagaga ggattatttc gccgcaatga gccgggcggg aataataaga
     5281 ttgcgtgaat gcgtctcttc atgggtgatt ttttgcatca tgcgatccgc aagtgtggta
     5341 ccaagttccc gcgcaggcgt gctggcccag gtaacaggta tatcatcaag tgtggttggc
     5401 gttgcatcgg taaatgccgc caacgaaacc tgttgttcaa agtaacgatc gactccgctt
     5461 tccccgcttt gccgccctgc tttcagcaaa ccaaaccatg cccccatcgc aatagtttcg
     5521 ttatagcaaa ccacagcact gatggtcggg ttatgacgta aaagcgccgt gatagcttcc
     5581 gcggcttgct tctggctgga agagcactcc aacacccaat cgctgtgaaa cggcaggcca
     5641 aattttagta gagttgcaca atagcccccc acccgttctg cccgggttaa tgaggaactt
     5701 tgccctccca gccaggcgat acgctgatgc ccattgcgaa tgagatgctc cgtcaacaac
     5761 tgtgcagcct gcatgttatc cgggcgaacc gtatcaacat catcaagata actggcacgg
     5821 gaagcgaaaa tcaccgggat agctttttct tctgccatcc gtcgcaggtc atcgctgctt
     5881 cctgcagccc cggcaatgac cacaccatcg accccctgat tcagtaacag tgaaaaccgc
     5941 tgtgccagct gctcgccgtc tttaccgccg tgaagcaaaa aaaccatccg tccctgcgct
     6001 tccagagctt ccgtcaatcc ggccgttaat tcggcgtaaa acggcgcaga taaatcacgg
     6061 acgatcaaac caatgacgcc gctttgcccg ccgcgcagcg ccgacgcctg acgattgcgc
     6121 acaaatccca gctcttcaat ggcggcgtta acgcgttctc ctgtggcggt agagattcgc
     6181 cctttgccac ttagcaccag cgaaacggta cttaccgaca cgcccgcagc cagcgcaaca
     6241 tcatgaatgg ttattttttt ggcggtagcc atgcagatga cctactccct gattatgtga
     6301 cagataaaac gttttacctt ttatttatct tatacccgct attatcgttg cgtaatgtga
     6361 tttatgcctc actaaaattt gataaaacgt tttatcttct cgcgcaattt actgaatcca
     6421 gattgttctc tacgaggagt cgttttatga cggcgaaaac agcaccgaaa gtcacgctgt
     6481 gggagttctt ccagcagtta ggcaaaacct tcatgttacc cgtggcatta ttgtcgttct
     6541 gcggcattat gctcggcatt ggtagttccc ttagtagcca cgatgtcatc accctaatcc
     6601 cggtcctggg caaccccgtg ttgcaggcta tctttacctg gatgagtaag attggctcgt
     6661 ttgcttttag tttcctgcca gtcatgttct gtatcgccat cccgctgggc ctggcacgcg
     6721 aaaacaaagg cgtagcggcg ttcgctggct tcgtcggtta tgcggtaatg aacctcgcgg
     6781 taaacttctg gttaaccaat aaaggcattc tgccaaccac agacgccgcc gttctgaaag
     6841 ccaataacat acagagcatt cttgggatcc agtcgatcga taccgggatc ctcggtgcgg
     6901 tgatcgccgg tattatcgtc tggatgctgc atgagcgatt ccataatatc cgcctgccgg
     6961 atgcgctggc attcttcggc ggtacgcgct tcgtaccaat catctcctcg ctggtgatgg
     7021 gccttgtcgg tctggtgatt ccattagtct ggccgatttt cgccatgggt attagcggct
     7081 taggccatat gatcaacagc gcgggtgatt tcggaccgat gctgtttggt accggtgaac
     7141 gtctgctgtt gccgtttggt ctgcatcaca ttctggtggc attaattcgc tttaccgacg
     7201 caggcggcac gcaggaagtc tgcggtcaaa ccgtcagcgg cgcactgacc atcttccagg
     7261 cgcaattgag ttgcccgacc actcacggtt tttctgaaag cgccacgcgt ttcctttcgc
     7321 aaggtaaaat gcctgcgttt ctcggcggcc tgccaggtgc agcgttagct atgtatcact
     7381 gcgcgcgccc ggaaaatcgc cataaaatta aaggtctgct tatttctggc ctgatcgcct
     7441 gtgtcgttgg cggcacgacc gaaccgctgg aattcctgtt ccttttcgta gcgccagttc
     7501 tgtatgtcat ccacgcgctg ttaaccggcc tcggcttcac cgtcatgtct gtgctcggcg
     7561 tcaccatcgg taataccgac ggcaatatca tcgacttcgt ggtgttcggt attttgcatg
     7621 gtctgtcaac caagtggtac atggtgccag tggtggcggc aatctggttt gtggtttact
     7681 acgtcatctt ccgtttcgct atcacccgct tcaatctgaa aaccccgggg cgcgatagcg
     7741 aagttgccag ctcaatcgaa aaagccgttg ccggtgcgcc gggtaaatca ggttacaacg
     7801 ttccggcaat cctcgaagcc ttaggcggtg ccgacaatat tgtcagcctc gataactgca
     7861 ttacccgtct gcgtttgtct gtgaaagata tgtcgcttgt taatgtgcag gcactgaagg
     7921 acaatcgggc aattggcgta gtacaactta atcaacataa cctgcaggtt gttatcgggc
     7981 cacaagttca gtcagtaaaa gatgaaatgg ccggtctgat gcatactgtt caggcataag
     8041 gataagatat gttcgatttt tcaaaggtcg tggatcgtca tggcacatgg tgtacgcagt
     8101 gggattatgt cgctgaccgt ttcggcactg ctgacctgtt accgttcacg atttcagaca
     8161 tggattttgc cactgccccc tgcattatcg aggcgctgaa tcagcgcctg atgcacggcg
     8221 tgtttggcta cagccgctgg aaaaacgatg agtttctcgc ggctattgcc cactggtttt
     8281 ccacccagca ttacaccgcc attgatacgc agtcggtggt gtatggccct tctgttatct
     8341 atatggtttc agaactgatt cgtcagtggt ctgaaacagg tgaaggcgtg gtgatccaca
     8401 cacccgccta tgacgcattt tacaaggcca ttgaaggtaa ccagcgcaca gtaatgcccg
     8461 ttgctttaga gaagcaggct gacggctggt tttgcgatat gggcaagctg gaagccgtgt
     8521 tggcgaaacc agaatgtaaa attatgctcc tgtgtagccc acagaatcct accgggaaag
     8581 tgtggacgtg cgatgagctg gagatcatgg ctgacctgtg cgagcgtcat ggtgtgcggg
     8641 ttatttccga tgaaatccat atggatatgg tttggggcaa gcagccgcat attccctgga
     8701 gtaatgtggc gcgcggagac tgggcattgc taacgtcggg atcgaaaagt ttcaatattc
     8761 ccgccctgac cggtgcttac gggattatag aaaatagcag cagccgtgat tcctatttat
     8821 cggcactgaa aggacgtgat ggcctttctt ccccttcagt actggcgtta actgcccata
     8881 tcgccgccta tcagcaaggc gcgccgtggc tggatgcctt acgcgtctat ctgaaagata
     8941 acctgacgta tatcgcagat aaaatgaact ccgcgctccc tgaactcaac tggcagatcc
     9001 cgcaatccac ttatctggcc tggattgatc tacgtccgtt gaatattgac gacaacgcgt
     9061 tgcaaaaagc gcttatcgag caagagaagg tcgcgatcat gccggggtat acctacggtg
     9121 aagaaggtcg tggttttgtc cgtctcaatg ccgggtgccc gcgttcgaaa ctggaaaaag
     9181 gtgttgctgg attaattaac gccatccgcg ctgttcgtta accccaattg cgcaacgtaa
     9241 aaaatcgttg cgcaatcgtg gatttttacc ctgctttgtt tttataatgg tgcgcacttt
     9301 tatatccaga aaaagagtgc gaccatgatt gataccaccc tgccattaac tgatatccat
     9361 cgccaccttg atggcaacat tcgtccccag accattcttg aacttggccg ccagtataat
     9421 atctcgcttc ctgcacaatc cctggaaaca ctgattcccc acgttcaggt cattgccaac
     9481 gaacccgatc ttgtgagctt tctgaccaaa cttgactggg gcgttaaagt tctcgcctct
     9541 cttgatgcct gtcgccgcgt ggcatttgaa aacattgaag atgcagcccg taacggcctg
     9601 cactatgtcg agctgcgttt ttcaccgggc tacatggcaa tggcacatca gctgcctgta
     9661 gcgggtgttg tcgaagcggt gatcgatggc gtacgtgagg gttgccgcac ctttggtgtg
     9721 caggcgaagc ttatcggcat tatgagccgg accttcggcg aagccgcctg tcagcaagag
     9781 ctggaggcct ttttagccca ccgtgaccag attaccgcac ttgatttagc cggtgatgaa
     9841 cttggtttcc cgggaagtct gttcctttct cacttcaacc gcgcgcgtga tgcaggctgg
     9901 catattaccg tccatgcagg cgaagctgcc gggccggaaa gcatctggca ggcgattcgt
     9961 gaactgggtg cggagcgtat tggacatggc gtaaaagcca ttgaagatcg ggcgctgatg
    10021 gattttctcg ccgagcaaca aattggtatt gaatcctgtc tgacctccaa tattcagacc
    10081 agcaccgtgg cagagctggc ggcacatccg ctgaaaacgt tccttgagca tggcattcgt
    10141 gccagcatta acactgacga tcccggcgta cagggagtgg atatcattca cgaatatacc
    10201 gttgccgcgc cggctgctgg gttatcccgc gagcaaatcc gccaggcgca gattaatggt
    10261 ctggaaatgg ctttcctcag cgcagaggaa aaacgcgcac tgcgagaaaa agtcgctgcg
    10321 aagtaacaaa aatggatggt gctaattgca ccatcctctt ttcacgcaag gcacagcgtc
    10381 gcgcgatgtt tggcggattc gatgcccagc tcaatcaact ccattacctg gattgcctga
    10441 cttgccggaa ccggattttc accatcgcca tttaacgcat cacgaatagc cgcatagtaa
    10501 gccggataat tccctggtac tgtcaacagc gtttcttcga cacgttcctc accttccacg
    10561 cgggtaagta cgccatcacg catatcgtag ccccagtctt cctgcggcag acgctcgcca
    10621 tttttcagac gttcttcctg tggatcgagg ccatatttca cataactgcc tcgggatcca
    10681 tgcacgatat aacgtgctga ctcagcggct gccagcatgg tgccgtgtaa aatgactcgc
    10741 cgctgcggat aggacaagat ggcgtggaaa taatcggtcg actgcgctcc gggccgtaac
    10801 tgtgccaaat ctaccgtcat gctaaccggt aaaccaaata gcgtaatggc ctgatcaaga
    10861 aggtgcggtg ctaaatcgta ccagataccg ctgccagggc cgccctgttc acgccaacga
    10921 tcgcgcacct gcggacggaa gcggtcaaaa tgagactcaa agtaagcaac ttcacccagt
    10981 acgccttccg cgagcaaacc ttttagcgtc aggaaatcgc tatcccagcg acggttatgg
    11041 aatacagaca gcacacgccc caggcttttt gccagcgctt ccagctctcg cgcttgtgac
    11101 agtgtcacgg taaagggttt atcaacgacc acatgtttac ccgcctcaag cgccgctttg
    11161 gctaacggga aatgggtatc gttgggtgta gggatgacaa tcaggtctat gttgggatcg
    11221 ttaaacagat gcttcggctc agagacaacc gtaaccgttg gccagtcggc ttttactttt
    11281 gtttcatcgc tgctggagat taccgccagt tccagcccgg gcgtgcccgc aatcaggggc
    11341 gcatggaagg ttttgctcgc ataaccatac ccaatcaacc caacacggat gttgtcgctc
    11401 atgtcattgc ctctcattga agtatgatgg ctatttgaca ctatccttta ccctcgctca
    11461 acagtttaat aacctgccag caataaggga tgttgtttaa cttaagtcaa aaaaatagcg
    11521 aattttccaa cgacaaaagc taaatatcgc aaaaacctca gtaaaaatct tgctggagct
    11581 attattgcta agtaacattt accccctgaa gttaatggat caatcaacag agatgtgggc
    11641 tgtaatgaat cgtcttattg aattaacagg ttggatcgtt cttgtcgttt cagtcattct
    11701 tcttggcgtg gcgagtcaca ttgacaacta tcagccacct gaacagagta ctacggtaca
    11761 acacaagtaa gctctgcgct tgtagagcga catgctgccc gtccgggtgc atcttttcac
    11821 ttgtcggata ttaaaccagg aatttattat cttgttcgat gttgttggtg attgtcagag
    11881 atagtaaagc gcgttactct tttcagagca gtttttcatc cctgaatatt tgccatcagc
    11941 gcattcttat tattgtaata acacacgcgt tcacttttgg tgaaatttta tcatcactaa
    12001 taatacctac ccctttggta ttaattattt tatggacctt ttatgactgt tcaggactac
    12061 ttattaaaat ttcgcaaaat cagttcactc gaaagtttgg aaaaactcta cgaccatctt
    12121 aattacaccc tgacggacga tcaggaactg atcaatatgt atcgtgctgc cgatcaccgt
    12181 cgcgcagagc tggtttctgg cgggcgtttg tttgacctcg gccaggtacc gaagtccgtc
    12241 tggcactatg tccaataaag aaagtagcga tcggttataa aagccttata ataacgcccc
    12301 taaaattaga tttgcctgtt tgcgtcgcta ttctggagaa cctatgacca ccacaacgcc
    12361 acaacgtatt ggaggctggt tgcttggccc attggcctgg cttttagtcg ctctgttaag
    12421 tacgacgctg gcgttgttgc tgtataccgc tgcgttatct tctccgcaaa catttcaaac
    12481 gctcggcgaa caagcactga cgacacaaat cttatggggc gtttctttta ttaccgctat
    12541 cgccatgtgg tattacacac tgtggttgac catcgctttc tttaaacgtc ggcgttgtgt
    12601 tcctaaacac tatattatct ggctgcttat ttccgtctta ctggcagtaa aagcgttcgc
    12661 tttttcaccc gtggaagatg gcatagccgt tcgccagtta ctgtttactt tgttggcaac
    12721 cgcattgatc gtcccctatt ttaaacgctc gtcgcgggtg aaagcgacgt ttgtgaatcc
    12781 gtaataacct tacagttaac ctgttgtcgc ctgctctgga ttaacggata ataggcggct
    12841 tttttatttc aggccgaaaa atgactgact acctgttact gtttgtcgga actgtactgg
    12901 tcaataactt tgtactggtc aagtttctcg gtctctgtcc gtttatggga gtttccaaaa
    12961 agctggaaac cgcgatgggc atggggctgg caacaacgtt tgtgatgacg ctggcgtcta
    13021 tttgcgcctg gcttatcgat acgtggattt tgatcccgct taatctgatt tacctgcgca
    13081 ccctggcgtt tattctggtg attgctgtgg ttgtgcagtt caccgagatg gtggtgcgca
    13141 aaaccagccc ggtgctttac cgtctgctgg ggattttttt gccgcttatc accaccaact
    13201 gtgcagtgct cggcgtggcg ttgctgaata tcaatctcgg gcacaatttc ttgcagtcgg
    13261 cgctgtacgg tttttccgcc gctgtcggtt tctcgctggt gatggtgctc ttcgccgcca
    13321 tccgcgaacg ccttgctgtg gctgatgtcc cggcaccttt tcgcggtaat gccattgcgt
    13381 taattaccgc aggtcttatg tctctggcct ttatgggctt tagtggtttg gtgaagttgt
    13441 aatgaatgct atctggattg ccgttgccgc cgtgagcctg ctgggcctgg cgtttggcgc
    13501 cattctgggt tatgcctccc gccgttttgc ggtggaagac gatccggtcg ttgagaaaat
    13561 tgacgaaatc ttaccgcaga gccagtgtgg tcagtgcggt tatcccggct gtcgccccta
    13621 cgcggaagct atcagctgta acggtgaaaa aattaaccgc tgcgccccag ggggcgaagc
    13681 tgtgatgcta aaaattgccg agttgcttaa tgtcgagcca cagccgctgg atggcgaagc
    13741 gcaagagcta acacctgcgc ggatggtggc ggttattgat gaaaataact gtattggctg
    13801 cactaaatgt attcaggcgt gtccggtaga cgccatcgtt ggcgctaccc gcgccatgca
    13861 tacggtaatg agtgatctct gtacgggctg caatttatgc gttgatccgt gcccgacgca
    13921 ctgcatctcg ttgcaaccgg ttgcagaaac acctgactcc tggaaatggg atctgaacac
    13981 cattcccgtg cgtatcattc ccgtggaaca ccatgcttaa gttattctct gcattcagaa
    14041 aaaataaaat ctgggatttc aacggcggca tccatccacc ggagatgaaa acccagtcca
    14101 acggtacacc cctgcgccag atacccctgg cgcagcgttt tgtcattcca ctgaaacagc
    14161 atattggtgc tgaaggtgag ttgtgcgtta gcgtcggcga taaagtattg cgcggccagc
    14221 cgcttacccg tggtcgcggc aaaatgctgc ctgttcacgc gcccacctcg ggtaccgtta
    14281 cggctattgc gccccactct acggctcatc cttcagcgtt agctgaatta agcgtgatta
    14341 ttgatgccga tggtgaagac tgctggatcc cgcgagacgg ctgggccgat tatcgctctc
    14401 gcagtcgcga agagttaatc gagcgcattc atcagtttgg tgttgccggg ctgggcggtg
    14461 cgggattccc gacaggcgtt aaattgcagg gtggcggaga taagattgaa acgttgatta
    14521 tcaacgctgc tgagtgcgag ccgtacatta ccgccgatga ccgtttgatg caggattgcg
    14581 cggctcaggt cgtagagggt attcgcattc ttgcgcatat tctgcagcca cgcgaaattc
    14641 ttatcggcat tgaagataac aaaccgcagg cgatttccat gctgcgcgcg gtgctggcgg
    14701 actctcacga tatttctctg cgggtgattc caaccaaata tccttctggc ggcgctaaac
    14761 aattaaccta cattctgacc gggaagcagg ttccacatgg cgggcgttca tccgatatcg
    14821 gcgtattaat gcaaaacgtc ggcactgctt atgcagtgaa acgtgccgtt attgatggcg
    14881 agccgattac cgagcgtgtc gtaaccctga ctggcgaagc aatcgcacgc ccgggcaacg
    14941 tctgggcacg gctgggtacg ccagtgcgtc atttattgaa tgatgccgga ttctgcccct
    15001 ctgccgatca aatggtgatt atgggcggcc cgctaatggg ctttaccttg ccatggctgg
    15061 atgtcccggt cgtaaagatt accaactgtc tgttggctcc ctctgccaat gagcttggcg
    15121 aaccacagga agaacaaaat tgcatccgtt gtagtgcctg tgctgacgcc tgccctgcgg
    15181 atcttttgcc gcaacagttg tactggttca gcaaaggtca gcaacacgat aaagccacca
    15241 cgcataacat tgccgactgc attgaatgtg gggcttgcgc gtgggtttgc ccgagcaata
    15301 ttcccctggt gcaatatttc cgtcaggaaa aagctgaaat tgcggctatt cgtcaggaag
    15361 aaaagcgcgc cgcagaagcc aaagcgcgtt tcgaagcgcg ccaggctcgt ctggaacgcg
    15421 aaaaagcggc tcgccttgaa cgacataaga gcgcagccgt tcaacctgca gccaaagata
    15481 aagatgcgat tgctgccgct ctggcgcggg tgaaagagaa acaggcccag gctacacagc
    15541 cgattgtgat taaagcgggc gaacgcccgg ataacagtgc aattattgca gcacgtgaag
    15601 cccgtaaagc gcaagccaga gcgaaacagg cagaactgca gcaaactaac gacgcagcaa
    15661 ccgttgctga tccacgtaaa accgcagtcg aagccgctat cgcccgcgcc aaagcgcgca
    15721 agctggagca gcaacaggct aatgcggagc cagaagaaca ggttgacccg cgcaaagccg
    15781 ccgtcgaagc cgctattgcc cgagccaaag cgcgcaagct ggagcagcaa caggctaatg
    15841 ccgagccaga acaacaggtc gatccgcgca aagccgccgt cgaagccg
//
LOCUS       DAPHCV010000107        15619 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.107, whole genome shotgun sequence.
ACCESSION   DAPHCV010000107 DAPHCV010000000
VERSION     DAPHCV010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.107"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            72..704
                     /gene="comR"
                     /locus_tag="RH033_003649"
     CDS             72..704
                     /gene="comR"
                     /locus_tag="RH033_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HDV2992776.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIARTTLRLWPELLK"
     gene            complement(766..1305)
                     /locus_tag="RH033_003650"
     CDS             complement(766..1305)
                     /locus_tag="RH033_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDV2992777.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMERD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1532..2836)
                     /gene="ndh"
                     /locus_tag="RH033_003651"
     CDS             complement(1532..2836)
                     /gene="ndh"
                     /locus_tag="RH033_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HDV2992778.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHSELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3167..3709)
                     /locus_tag="RH033_003652"
     CDS             complement(3167..3709)
                     /locus_tag="RH033_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV2992779.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3732..4757)
                     /gene="nagZ"
                     /locus_tag="RH033_003653"
     CDS             complement(3732..4757)
                     /gene="nagZ"
                     /locus_tag="RH033_003653"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HDV2992780.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAREAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHSDPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(4768..5592)
                     /gene="thiK"
                     /locus_tag="RH033_003654"
     CDS             complement(4768..5592)
                     /gene="thiK"
                     /locus_tag="RH033_003654"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HDV2992781.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPRFAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRRLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTDQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5573..6214)
                     /gene="lpoB"
                     /locus_tag="RH033_003655"
     CDS             complement(5573..6214)
                     /gene="lpoB"
                     /locus_tag="RH033_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HDV2992782.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQRESAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6228..6605)
                     /locus_tag="RH033_003656"
     CDS             complement(6228..6605)
                     /locus_tag="RH033_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HDV2992783.1"
                     /translation="MRKGCFGLVSLALLVLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTLPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6608..6967)
                     /gene="hinT"
                     /locus_tag="RH033_003657"
     CDS             complement(6608..6967)
                     /gene="hinT"
                     /locus_tag="RH033_003657"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HDV2992784.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7301..9490
                     /gene="fhuE"
                     /locus_tag="RH033_003658"
     CDS             7301..9490
                     /gene="fhuE"
                     /locus_tag="RH033_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HDV2992785.1"
                     /translation="MLSTQFNRDNQYQTTSKPSLLAACIALALLPSAAFAAPATEETV
                     IVEGSATTPVDDENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWAYIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(9550..10983)
                     /gene="ptsG"
                     /locus_tag="RH033_003659"
     CDS             complement(9550..10983)
                     /gene="ptsG"
                     /locus_tag="RH033_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HDV2992786.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11278..12075)
                     /locus_tag="RH033_003660"
     CDS             complement(11278..12075)
                     /locus_tag="RH033_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDV2992787.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12086..13090)
                     /gene="holB"
                     /locus_tag="RH033_003661"
     CDS             complement(12086..13090)
                     /gene="holB"
                     /locus_tag="RH033_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDV2992788.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDIWQARETLCQ
                     ALAYSVQSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13087..13728)
                     /locus_tag="RH033_003662"
     CDS             complement(13087..13728)
                     /locus_tag="RH033_003662"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDV2992789.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTNWVKELDA"
     gene            complement(13718..14740)
                     /gene="yceG"
                     /locus_tag="RH033_003663"
     CDS             complement(13718..14740)
                     /gene="yceG"
                     /locus_tag="RH033_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HDV2992790.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVSSERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14743..15552)
                     /gene="pabC"
                     /locus_tag="RH033_003664"
     CDS             complement(14743..15552)
                     /gene="pabC"
                     /locus_tag="RH033_003664"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HDV2992791.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLELEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
BASE COUNT         3895 a         4187 c         3805 g         3732 t
ORIGIN      
        1 gtggactggt gaacggaggt gattattttt ataataacct ttcattcaca gtcaccaggt
       61 acaacggcat catggcaact gactcaacac aatgtgtaaa aaaaagccgc ggccgcccaa
      121 aagtgttcga cagggatgcc gcgcttgata aggccatgaa attgttctgg cagcacggtt
      181 atgaagcgac ttctcttgcg gacctcgtcg aagcgaccgg agccaaagcg cccacgctat
      241 acgcggaatt taccaacaaa gaggggttat ttcgcgccgt actcgaccgt tatatcgatc
      301 gttttgccgc taagcatgaa gcacagctgt tttgtgaaga gaaaagcgtg gagtctgcgc
      361 tggctgacta ttttgctgcc atcgccaact gctttaccag caaagacacc ccggcaggct
      421 gcttcatgat caacaactgc accaccctct ccccggattc aggagatatc gccaatacgc
      481 tgaaatcacg ccatgcgatg caagagcgca ctttgcagca gtttttatgt caacgacaag
      541 cgcgcgggga aatcccgacc cactgtgacg tgacgcatct ggcagaattc cttaattgta
      601 ttattcaggg gatgtcgatc agcgcacgcg aaggtgcatc gctggaaaaa ctgatgcaaa
      661 ttgcccgaac gactttgcgt ttatggccgg aactgctgaa ataagtcgca aaaaaagccc
      721 ctcagcctga gcgaatgagg ggccaaattg cagagtacag ccttgttata ctttgttatt
      781 caaaatcagt tgcccgttgc tatctagcgg gatctgcgta cccggatcgc gttccatgcg
      841 gattttgccc tgctgatcgc caatcttata ggtcacatca taaccgagca ttttttctga
      901 cttgtcatac accgttttac aacgctgttg cgtagtcgtg taagtatcgc tttcctggag
      961 agagccctgg atctggttac cggcatatcc accacccagc gcccccacaa cagtagcgac
     1021 atctttaccg cgaccaccac caaactgatg ccctatcacg ccaccagcaa cagcgccgag
     1081 caccgacccg gtaatgcgat tttcatcctg caccggtcga cgatgggtca ctgtgacgtt
     1141 gcgacactcc tgacgcggtg ttttaaccgt ttccttgatt ggggttgcag aaacaacctg
     1201 agcgtattgc gggccacgtt caaacacgtt cagactggcc actgccgcta cgcccaacgc
     1261 agctgcgaca ccaatcccga tacccgccaa cattgattta ttcacgggaa tacctccttc
     1321 tttgcagtta tgccaatcat gcaagcgaag gagaaaagca gctatcagac agaagatgaa
     1381 aagcgcggga atggctggaa atacttaggg attcggagga gtctgaagga atgcctgatg
     1441 cgacacttaa cgcgtcttat caggcctacc gctcgtacca tttgtccagg gcggataagg
     1501 cgtttatgcc ccatccgcca gtacaagccg attaatgcaa tttcaaacgt ggacggataa
     1561 cgcggttaat actccccacc agcatcatta atccggtttt aaagtaacca tgcagcgcaa
     1621 tctggtgcat tcggtacagc gagatgtata caaagcgcgc aattcgtcct tcaatcatca
     1681 ttgagccgcg tgtcaggtta cccatcaggc taccgacggt ggaaaagttc gacagcgata
     1741 ccagcgaacc gtgatcttta tactgataat ttttcagcgg cttaccgttc atctgcgcca
     1801 gaatgttgtt cattgcgcag gttgccatct gatgtgcggc ctgagcgcgc ggcggaacaa
     1861 agcccccttc cggacgcggg catgacgcgc agtcgccaat agcgtatatg tctggatcgc
     1921 gagtggtttg cagcgtcggt tccaccacca gctggttgat acggttggtt tcaagaccac
     1981 cgatatcttt caggaagtct ggcgctttga tcccggctgc ccacaccatc agatcagcct
     2041 caatatattc gccatcttta gtgtgcaggc cgccttcatc agcactggtg accatggttt
     2101 gcgtcagcac gcgaacgcca agtttcgtta actcactatg ggccgcagca gagatacgtg
     2161 gcggcaatgc aggcaaaata cgttcgcccg cttctaccag cgttacgttc agggcttcgt
     2221 tggtcagacc tttataaccg taactgtgca gttgcttgac cgcgttgtgc aattcagcgg
     2281 agagttctac acccgtcgcg ccgccgccga caatcgcaat gtttactttg ccattcgcgc
     2341 ccaggttggc ggagtatttc aggaacagat tcagcatctc ttggtggaag cgacgcgcct
     2401 ggtgcgggtt atcgaggaaa atgcagttct ctttgacacc tggcgtattg aaatcgttag
     2461 aggtgctacc cagcgccatt accagggtgt cataggcgat tttacgttcc ggaaccagca
     2521 gttcaccttt ctcgtcgcgc agttctgcaa tagtgattgt tttcgcttca cgatcaatat
     2581 caatgacgga acccagctgg aactggaaac catgattgcg cgcatgggcc agatagctca
     2641 acgcatcgac gccttcatca agcgagccag tcgccacttc gtgcagcagc ggtttccaca
     2701 ggtggctgtg gttacgatcg accagcgtga ttttggcttt tttcttgcgt cccagcttat
     2761 gccccagctg tgttgccatt tccagcccac cagcaccgcc gccgacaatc acaatttttt
     2821 tcaatggcgt agtcaacgtg acccccttaa atttattaac caattgttaa ttaatggtta
     2881 ttaacatagc tcttaattaa caacaggtta caacaatgaa aatgttcttc actattgaga
     2941 ataacatgaa tggtgcattg gtcataccaa aattgatgtt aatcaagttt tgttgctgaa
     3001 aagataggcg actttaccgg gcgagatcaa aaaacagagc ttattgttta ttatttgagc
     3061 gaatttatga gagtggtata gcgtgagggg tgtatggagg acgtttggta ggcctgataa
     3121 aatgcgcatc aggtaccggc aaggttacct gatgcgacga gggcatttac ccgagggttt
     3181 tgaacgcttt aatacgctgt aaatgcgggg agatattctt gaatttgtgc gtctgctctt
     3241 cgtcccagac aatctcgtaa taatgatgca gctcttcaga tgtccgctgg ctgttaagcg
     3301 cttcatcatt acgcgacaaa atcaccaggc aacgatcgcg attcttttcg cggaagttgg
     3361 tcacacactt agtggcaata tcggcatact cttccgggcg atcaatcttg ccttccatgt
     3421 tctcataagg gaacaaatta gggttgaaga tcacctggcg gatatcgcaa agaaaaccaa
     3481 tccgttccgc ccagtatccg cccagaccaa cgccgcaaat cagcggacgc tcgtcgacgt
     3541 tcagttgcaa cattttgtcc acttctttga gcagatgctg catatcatgt ttcggatgcc
     3601 gcgtactgta gcttatcaag cgtacatccg ggtcaataaa ctgcaattgt aagactttct
     3661 cgtggttacc cggactgtta gagtcaaaac cgtgtaaata gataatcatc gcttcctcac
     3721 ataagccagg gttagtgacc tgctttctct tcctgccagc gttcatgtaa ctgattcaga
     3781 cgggcgctga tcgctttcca gcgagccgag tccatcagtt cctgtcgcga aaatgaacct
     3841 ttatgataca aacgtgtaac acgttctgcc ttgatcggtg acagattatc taacacgctg
     3901 acggcccctt tacgattatt gcagaccagg atcatatcgc aacccgcgtc cagtgatgcc
     3961 tgcccgcgtt cggcataact gcccataatc gcggctcctt ccatcgataa atcgtcagag
     4021 aaaatcacgc catcaaaacc cagttcctga cgcaaaacgg ttttcagcca atagggagaa
     4081 ccgctcgccg gacgcggatc gacatcactg tagatcacat gcgcaggcat aatggcgtcg
     4141 agtttatttt cgcggattaa ggaactgaag accgacatat ctttagcgcg aatctccgct
     4201 tgcggacgtg ggtcgcacgg tgtttctttg tgtgagtctg ccgttactgc accgtgtcct
     4261 ggaaagtgtt tcccggtcgt tttcattccg gcttcatgca taccatcaat aaaccggctg
     4321 gcaattgcca gggctttttc gggatcggaa tgataagaac gctcgccaat cgccgcgctg
     4381 atatgcccga cgtccagcac tggcgcaaag ctgatatcaa tatccatagc aatcatttcg
     4441 ctggccatca accaacctgc ctctcgcgcc agttttccgc cttcttccat tcccaacagc
     4501 gcagcaaatg attgtgccgc tggcaagcgg gtaaaacctt cacgaaaacg ctgcacgcgt
     4561 ccaccttcct gatcaaccgc caccaccaga tgattgcgcg aagctgcgcg gatctggcgc
     4621 accagttcac gtaactgggc aggatcatga tagttacgcg taaagagaat cagccctccc
     4681 accagcggat gcgccagtat ttcacgctct tccgcgtcca gttcgtaacc ttcgacatcc
     4741 aacattactg gacccacact gctctcctta ttgttttatt aatagctgcc gccaggtgtc
     4801 atcggccagc ctgataaatt gttgatcgcc ggtttgtcgc cagcggtact caaaccaccc
     4861 tgctttgagc atcagcagcc agggaaacca tcgcctgacc tgacgccata attgcgccgg
     4921 ataaatcttc gcgcgagtgg catagtcatt gaccaattgc cggtgctggt cagtattttc
     4981 cacccacacc gccgccagtt ccagcgcgat atcaccatct ccggcatact cccagtcgat
     5041 gagttttaac cctgacgcgc tatgcactaa atttccggcg tggacatcca tatgcaatgg
     5101 acttaagcgt aaaggccgtg gttcccgcgc tttgcgcaga cgttttaacc ttcgcagcca
     5161 acccactgtc cgccgcgccg gatcgctttg ctgccagtac agttccagta acggcaacag
     5221 cgttattcgc cagccaaaac gcggttgttg atgtagataa tacagcaagc ctgccagttc
     5281 gttggtatcc ggcaaatacg tttttacctc gccgggcaga tagtcgacca ccatccagtc
     5341 acggagatat aaatgcggct tcggtgcaat gcatgcgggt agttgtgata aagcccgata
     5401 ctggcgcaag aacgcgaacc gcggcgcatc aggatcgtgc ggctgacgca caacaaaacg
     5461 ctggccctga tgttcaatga gaaaactccc gccactaagc ccactattaa acgtcgtgac
     5521 gggatgaaac tgcgggaaaa agcgcgacag caattcgtcg cgcgtgatgg gattattgct
     5581 gcgaaacggc acctttacct gaccagataa tttcgcccgt ctgcaccagc atcagctgca
     5641 tttgaagggt cggagcgtta acgttgccag aggcgctgga gtacagcacg taatgagcgc
     5701 cgacattgcg ggcaatgcct atggctttac tacgggtgcc taaactgtcc tgcggcgaca
     5761 aacctaactg ttgctttgcc atcgacagct gctgggcgga aaccagggta aatttaccgt
     5821 tattagccag cgcatttcgc agcgtttcgg tcgcttctgc ggcattcagc gaaccgttag
     5881 tacggttgtt aacgctatca accagcagga cgctacccgc agtcacgccg tcagccccaa
     5941 gcatcttact gaccatcggc tgcattgcgc cattccaatc ataatggcga atatgcggcg
     6001 caggcggtgc agtttgatct tcgtgctcaa ttgggcctgg ctgctgcggg attgtcggca
     6061 ccgatggcac tgtggggaca ggctgttgcg gctcggctgg ttgttccggc gctggtttca
     6121 cttcttctac cggcgcagat tcacgttgcc ccacacaccc ggcaagaaac atcgccagcg
     6181 cggtaatcaa ggcgtagcga ctcatttttg tcatcaagat tcacccctta caaatataga
     6241 taaagtctga ctttgtgcgc ccccagaaaa ttggcgctgc cgtacagcgt taccgccgaa
     6301 tgtgcgggaa gggtaacgct gcgtggcctt tccagaggat gcatctccag ccctctggcg
     6361 tcataccagt aaaaacgata atgaacggta acgggttctt gcctttcgtt ataaagcgtt
     6421 gaggatgctg aaggttgaat atcagacgtc gaaaggacgg gcttttctgc actgatgccc
     6481 gcagccaata aagatgactc catcaccagc gattgctcat cattcaccgg aatttccgga
     6541 tgtgaacgac agcccaccag cactaacaac gccagagaca ccagcccaaa gcatcctttt
     6601 ctcattgtta cagaccttta tgcgccagca ttggtcccag cggacggcca cccaacaagt
     6661 gcatatggat gtggtaaacc tcttgtccgc catggcggtt ggtgttcatg atcagacgat
     6721 agccatcttc ggcaatacct tcttgctcag caatttttgc cgctacggtg atcatgcgtc
     6781 ccagcgcctg ctcatgctca gctgacacgt cgttcacagt cggaatgagg atattcggaa
     6841 tgatcagaat atgcgttggc gcttgcggcg aaatatcgcg aaacgccgtt accagatcat
     6901 cctggtagac gatatcggag gggatctcac gacgaataat tttgctgaat atagtttctt
     6961 ctgccacgac attttccttt ttcaaatagc catgcgttgc gccatgctac gccgcgccac
     7021 actgcgagta tagagtatgg tcgagttaaa cctgctcttt caaccttaac tcacgatttc
     7081 ttaagcaaaa aatcgttttg ctgccgtata tatcgccatt attcccattt ctgcctgcga
     7141 atgcgctgaa aagacattct ctgtttggtt tacacgtcaa atatgaatgc gtatatttct
     7201 catttgcatt tacatgcaaa attattcgca cactaaatat aaccgctcat caccctcctc
     7261 cggcatgagc ctgacgacaa cataaaccaa gagatttcag atgctttcaa cacaatttaa
     7321 cagggataat caatatcaaa ccacctccaa accgtcactg cttgccgcct gcatagcact
     7381 ggcactctta ccttccgccg cttttgctgc accagccact gaagaaacgg tgattgttga
     7441 gggttcagcc actactccag ttgatgacga aaatgattac agcgtaacgt ctacctctgc
     7501 gggtaccaaa atgcagatga ctcaacgtga tattcctcag tcggtcacta ttgttagtca
     7561 gcagcggatg gaagatcagc agttacaaac gctgggcgaa gtgatggaaa acacgctggg
     7621 gatcagcaaa agccaagcgg attccgatcg tgctctttat tattcccgcg gattccagat
     7681 cgataactat atggttgatg gtatccccac ctattttgaa tcgcgctgga atctgggcga
     7741 cgcactttct gatatggcac tgtttgaacg cgtagaagta gtgcgtggcg cgacaggact
     7801 catgaccggg acgggtaatc catctgcggc aattaatatg gttcgaaaac acgcgaccag
     7861 tcgtgaattt aaaggcgatg tctcggcgga atacggtagc tggaacaaag aacggtatgt
     7921 ggcggattta caaagcccac tcaccgaaga tggtaaaatc cgcgcacgaa ttgtcggcgg
     7981 ctaccagaat aacgactcat ggctggaccg ctacaacagt gaaaagacct tcttctccgg
     8041 cattgtcgat gctgatttag gcgatcttac gacgctttca gccggttacg aatatcagcg
     8101 cattgatgtt aatagcccaa cctggggcgg tttaccgcgc tggaatactg atggcagcag
     8161 caacagttac gatcgcgcac gcagtaccgc acctgactgg gcgtataacg ataaagagat
     8221 caacaaggtc tttatgaccc tcaagcagcg gtttgctgat acctggcaag cgacactgaa
     8281 tgccacccac tccgaagtcg aatttgacag taaaatgatg tatgtcgatg cctatgtaaa
     8341 caaagcggat ggtatgctgg ttgggccata cagtaattat ggacctggct ttgattatgt
     8401 cggcggtacc ggttggaaca gcggcaaacg taaagttgat gcgctggatt tgtttgctga
     8461 cggtagttat gaattgtttg gtcgtcagca caatctaatg tttggtggca gttacagcaa
     8521 acaaaacaat cgttacttca gttcatgggc ctacatcttc ccggatgaaa ttggcagttt
     8581 ctacaacttt aatggcaatt tcccacaaac cgactggtca ccacagagcc tggcgcagga
     8641 cgataccaca catatgaaat cgttatatgc tgccactcgt gtcacccttg ccgatccgct
     8701 gcatctgatc ctcggcgcac gttataccaa ctggcgggtt gatacgctga cttacagcat
     8761 ggagaaaaac cacaccacgc cttacgctgg tctggtgttt gacatcaatg acaactggtc
     8821 gacctacgcc agctatacct ctattttcca gccgcaaaat gatcgtgaca gttcaggcaa
     8881 atatctggct ccaatcaccg gtaacaacta cgagctgggt ctgaaatcgg actggatgaa
     8941 tagccgtctg accaccacgt tagccatctt ccgtattgag caggataatg tcgctcagtc
     9001 caccggtaca cctatccccg gcagcaacgg cgaaaccgcc tataaagcgg tggatgggac
     9061 agtcagtaaa ggtgtggaat ttgaactcaa cggcgcaatt accgacaact ggcagctgac
     9121 gtttggcgca acgcgctata ttgcagagga taacgaagga aacgccgtta atcctaatct
     9181 gccacgcacc acggttaaaa tgttcaccag ctaccggttg cctgtcatgc cagagttgac
     9241 ggtcggcggt ggtgttaact ggcaaaatcg cgtgtatacc gacaccgtga caccgtatgg
     9301 caccttccgc gccgagcaag gtagctacgc gctggtggat ctcttcaccc gctaccaggt
     9361 gacgaaaaac ttctcgttac aggggaatgt caataacctg ttcgacaaaa cctacgatac
     9421 caacgtggaa ggttctattg tctacggcgc accgcgtaat ttcagcatta ccggcacgta
     9481 tcaattctga ataacacctg taaaaaaggc agccatctgg ctgccttagt ctccccaacg
     9541 tcttacggat tagtggttac ggatgtactc atccatctcg gttttcaggt tatcggattt
     9601 agtaccgaaa atcgcctgaa caccagaacc agcaaccact acgcccgctg cgcccagttt
     9661 cttcaggcca gcctgatcca ctttagacac atcagcaacg ctgacgcgca gacgggtaat
     9721 acatgcgtcg aggttagtaa tgttttcttt accaccaaat gcagcaacca gagccggtgc
     9781 catttcgctg gtacctgtcg cttttgcgtc ttcagtcgcg tcttcacgac ccggcgtttt
     9841 cagatccagt gctttaatca gcacgcggaa gatggtgtag taaacaatgg cataaccgat
     9901 accgacgatc gggaacagcc acagtttgct gctgttacca gacagaacga tgaagtcgat
     9961 cagaccgtgc gagaacgacg taccgtcacg catccccaga agaatacaga ttgggaatgc
    10021 caggcctgcc agaatcgcgt ggatgatgta caggatcggc gcaacgaaca tgaaggagaa
    10081 ctcgatcggc tcggtgatac cggtcaggaa cgaggtcagc gccgcggaga tcataatacc
    10141 gcccactttc gcgcggtttt ctggtttagc agagtgccag atagcaattg cggcagctgg
    10201 cagaccgtac attttgaaca ggaagccacc agacagttta cccgcagtcg ggtcacccgc
    10261 catataacgc ggaatgtcgc cgtggaaaac ctgacctgct gcgttggtgt attcaccaat
    10321 ctgcatctgg aaaggtacgt tccagatgtg gtgcagacca aacggtacca ggcaacgttc
    10381 gatgaaaccg taaatgccaa acgcaactac cgggttctgg taagcagccc actgagagaa
    10441 ggtctggatt gcagaaccaa tcggcggcca aatgaaggac agcacaacgc cagtaaagat
    10501 ggcagccagg cctgaaatga tcggcacaaa gcgtttaccg gcaaagaagc caagatactc
    10561 aggcagctta atacggtaga aacggttaaa catgtacgct gcgatcgcac cggagataat
    10621 ccccccgagt acgccagtat ccgccaggtg tttagaggcg atttcttcag caggtaaatg
    10681 cagtaccagt ggcgcaacca cggccatggt tttaaccatg atgccatagg caacaactgc
    10741 ggccagcgcg gatacgccat cgttattggt aaagccgagg gcgacaccga tcgcaaaaat
    10801 cagtggcatg tttgcaaaga cggaaccgcc tgcttctgcc ataacatgcg atacaacggc
    10861 gggcagccag ctgaaattcg cggaaccgac gcccagcaga atacctgcga taggcagtac
    10921 ggataccggc agcatcagcg atttaccgac cttttgcagg ttagcaaatg cattcttaaa
    10981 cataattgag agtgctcctg agtatgggtg ctttttttac gttctcacgc gtggcaaggg
    11041 gggagtgcct cgccgtgtac agggcatcta agcgcccttt atttattaca cagagtaaaa
    11101 taattcagtg ccgatttgtt tgacggctat cacgtttcaa cttcagatca ttggagaagt
    11161 tgcacatttt gacaaaacat atgtcacaat atttcaaacg tggttattca ccgcccgatt
    11221 ttcggcggtg aactttactc gttttagcca ttaattacga gctttaaaaa aactcattca
    11281 acggatggat tgaaggcggg aagcgtcgat gtgaaacaga cgggcgaagt tatcggtggt
    11341 tacctgcgcc agttcttcaa cggcaacacc tttcaacaca gccatgtatt ctgcaacgtc
    11401 acgaaccatc gcaggttgat tctcttttcc tcgatgcggt accggcgcaa ggtaaggtga
    11461 gtcagtttcc accagtaacc gatccagggg gacataacgc gcagcatcgc gcagttgctc
    11521 cgcattacgg aaggtcacaa tgccggaaaa ggagatgtaa aatccgagat ccagtaattt
    11581 gcccgccgtt tctctgtcct ctgtaaaaca gtgtagtacg ccaccgcaat ccgtcacttt
    11641 ttcttcgcgc agaattgcca gcgtatcggc gcgggcgtca cgggtgtgga cgataaccgg
    11701 cttgttcagt tcacgaccaa tctggatatg atggatgaag gactcttgct gacgtacttt
    11761 agtttccggc gtgtaataat aatccagccc ggtttcaccc agcgcaacaa cgccctcttc
    11821 tgccgccaga cggcgtaaat cttctacatc gtagggatca ttctggttga gtggatgcac
    11881 gccacaagaa aatacgacgt tgtcacgttc gcctaccaga tcccgcatgt gtaaataacc
    11941 tggtaatgtt gtggcgactg ccagacaaaa tttcacatcg cgtgcggcgg ctttcgccag
    12001 aacgtcatcc acgtccttat gcaaagattc ataatccaga ccatcgagat ggcagtgtga
    12061 gtcgactaaa aacatgatgt ctctcttaaa gatgaggaac cggtagcaca acgcccggtt
    12121 gcaggtaatg ctcaatacgc agtaaaagat cggtgatgag aagctcgcgg ttaatacctg
    12181 tgacagacat taactgttca cgaatgtggc aaacatcccc cagtatagcc tgcagacgcg
    12241 agggagaaag atggtttgcc agttcgacga ccaggcccgg cacatcaaca ttggtaacct
    12301 gcgcagcgcc atgatggcgt tttagcgcat ccatcagcaa cgttgccagc cagtgtaaac
    12361 gcgccggagc ttgttcatga ttaagggctg ctagcagcga ataccagtcg cccgattgca
    12421 cgctatatgc cagcgcctga cacaatgttt cacgagcctg ccagatatct ccctgaaaca
    12481 acgccagtgc cgcaccaggc gaaccggcgc ttaagcgcaa tgcggcaagt aatgcatcct
    12541 gtgacattgt cacttcgcgt gaaagccagg tcacggcgta ctgttccggt ggcggcgcaa
    12601 ggtaatgtaa ccgacaacga ctacgtaatg ttgccagtaa acgttctggc tcgcgggtag
    12661 caaggaaaaa ccacgtttct gctggtggct cttcaagcgt tttcagcaat gcgttagccg
    12721 cggcgtcggt tagtaaggca gcatcggtta cccagacaac tttcgcacca cctaagcgtg
    12781 cgtgctcatt cagcttttcg gtgacctcac gtaccgcatc aatgcccagc gtatttttcc
    12841 ctttttcggg agccagggtg tagtaatcgg gatgcgtgcc agcctgcatc aactgacatc
    12901 cacgacagtg accgcaactt ttgtggccct gcggttgttg gcagagtaaa taacggctca
    12961 gggcgtagat taaagcatca tcgcccatgc ccggtaacgc ctgaatgagc agcgcatggt
    13021 gacctcttcc ggcctgatag ctggctacca gtttttcgaa atcaggtcgt aaccatggat
    13081 accatctcat gcgtccaact ccttcaccca gttggtcacg gtagtgcgga ttgcatccat
    13141 cacagcctcc agcggctggg tggcatcaat ggtatgaatg cttttatctt gtgctgccag
    13201 ttccagatag cgggcgcggg tgcgattaaa aaaatcgaaa gactcttgct caatacgatc
    13261 cagctcgccg cgtgcacgcg cgcgttttaa gccaacttcc ggggtaacat cgagatagag
    13321 cgttaagtcg gggcgaaaat ccccaagaac agcatcacgc agtgttgcca gcatatgttg
    13381 gtcaatacca cgtccgccgc cctgatacgc ctgagtggag agatcgtggc gatcgccaat
    13441 cacccaggtg ccgttagcca gcgcaggttt gatgaccgtt tctaccagtt gaacgcgcgc
    13501 ggcataaaac atcagaactt cggctttatc ggtaatgact tcatcgccta ccgatttgat
    13561 atccagcacc aggcttctta acttttcggc aagttgcgta ccgccaggtt cccgagtgaa
    13621 aaccatgtcg cggataccca gttgctcgag cgtctcaacc accacgttac gcgcggtagt
    13681 tttgcctgcg ccttccagcc cctcaatgac gatatactta ctgcgcattt ttttccttaa
    13741 gcactttcag ataatcctgc acagacttgt tatgactggc aagattggta ttaaacgtgt
    13801 gaccaccttt accatcggcc acaaaataga gatacggcgt ttttgccgga tgcgcagcag
    13861 ccttcagcga atccgccccc ggcgtcgcta tcgcacctgg cggcaaaccg gtaatggtat
    13921 aggtgttata cgctgtcggc gtttccaggt ctgcacgaga aagtttgcca ttataacgct
    13981 ctcccatccc gtaaatcacg gttgggtcgg tctgcaggcg cataccaatg cgtaaacggt
    14041 tgataaatac cgaggcaacc tgatcgcgtt cgctggaaac ggcggtttct ttttcgataa
    14101 ttgatgccat cgtcaccaac tggtttttat ctttataagg cagaccgtcc gcacgccctt
    14161 cccaggcgct atcgaccgct ttcaccattt tcttgtgcgc tcgcttgagt aacgcgacat
    14221 cggtggtatt ggcggtatac atccaggtgt ctggccagaa ccaaccttca atccactccg
    14281 ggttttccag ttcaagtgcc tgcgctacgg tggcgtactt atcgtcgctc agcgtgtgct
    14341 tgatataggg ggcctcacgc aattgcttga ggtaatcgct cagacgcatc ccttctacca
    14401 gtcgcagagg gaactgtgct tctttaccgc tttccagcaa tttcagcatc tcgcgcacgg
    14461 tcatctgcgg tgtaaagcgg taagtcccgg ctttaaagtg agaaagatcc ggttcgatac
    14521 gcagcagcca ttggaaaacc cgtgggcgat tgatgatctt atcggcataa agctgttcac
    14581 cgagcgccag acgtccggtc cctggcttca gggtaaatat cgtctcttct ttgataagta
    14641 atttgctgtc ggcaagatgg cgaaccttcc agacgcccac accagcggcg atacccagta
    14701 ccaccagcaa taacaagata atcaataaca cttttttcat gactaattcg ggcgctcaca
    14761 aagtggggct aaatattcat ataacgttgc tgacgaaaag gagacatcgc cacaggcacg
    14821 tacgggcatc actggcatta acgcattaca aataaccatc tcatctgcct gcaacgcctc
    14881 ctccagcgag gcttgcactt cgacaagctg ataagaggat tgtgccagca aacggataca
    14941 gaattgtcgc ataatgccgt taacacctgc ctgatccagt cgcggcgtat aaactacgtt
    15001 gcccttccgc cagaacaaat tagccgcaca gcattccgta acccaccctt cgctgtcaag
    15061 gaccagcgcc tcatcagcgt ttgtctgctc aagatgagag cggatcaata cttgctcaag
    15121 ccgattaaga tgtttaatac ctgcaagatg aggattgcgc cccagccgca ccgggcttag
    15181 cgccaacgtc atcccctcgt tacgcaaacg gtcgtaatgt gcgggataaa ccgtaacgga
    15241 gagaatccgc gttgccggtc cgctgttcaa tgtgctgtat cctcgcccgc cactaccgcg
    15301 actgatcacc actttcagca caccattttg ctgttctgct gccagcgttt tcatctctag
    15361 ttcaagctga ggccagaagt cacaggaaat catcaaccgc tgacaagcat cctgtagtcg
    15421 ctggatatgc gccgataaca aactgacttt accgtcgata actctggcgg tggtaaaaca
    15481 accatcacca aactgcgttg cccgatcgct tactgccagc gattcctgct tatgaccgtt
    15541 aattaagaac ataccggctc cttatcagag aattgttagt gtggcagcat gttcactacg
    15601 gaacaagtcg gaataaaag
//
LOCUS       DAPHCV010000108        15401 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.108, whole genome shotgun sequence.
ACCESSION   DAPHCV010000108 DAPHCV010000000
VERSION     DAPHCV010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.108"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1062..1286
                     /locus_tag="RH033_003665"
     CDS             1062..1286
                     /locus_tag="RH033_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001470144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992792.1"
                     /translation="MSENTGKSHENKMVIIDCGEYDEYLRNAWFRGFREGYDMAMKFG
                     LGCQQCYETGEKIACARAEECRKKIKVIKH"
     gene            complement(1415..1939)
                     /locus_tag="RH033_003666"
     CDS             complement(1415..1939)
                     /locus_tag="RH033_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HDV2992793.1"
                     /translation="MKRKLIAFVILATSSCGYAATKDVTMNLVDSDGKERNIGKITIQ
                     KTPYGLLFTPDLHSLPAGIHGFHVHENASCAPALKDGKTVAALSAGGHFDPEKTGKHL
                     GPWSPDGHLGDLPALYVTHDGKATYPVLAPRLSELKELDGRSLMLHAGGDNHDDHPEP
                     LGGGGARIACGIIP"
     gene            complement(2035..3096)
                     /locus_tag="RH033_003667"
     CDS             complement(2035..3096)
                     /locus_tag="RH033_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004919977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDV2992794.1"
                     /translation="MILAGGLLCSSLATAADSPDLIAQAKKEGSVTFNVWYLQPQWRS
                     FVKDFEQQYGVKVRIPEGNIDGNVNKLLAEAGKKRGKIDVIALSVAQLPVAMGAKALA
                     KVDDLPGYGDAIHQIQNVDTQGYAVAFWGNQTGFAYDPQQMGDRALPQTLEQLQSFID
                     ANPKKFGYNDPNNGGAGEAFIQRIVTTQGDTFNSEAESVDPAVVKNWQKGWQWFAANK
                     DKITRTASGADSLTRLNDGELMLTPSWEDHLVGLQKTGAINSRLKFYVPEFGMPGGGN
                     IAAIAANSPHPAASRLFLNWLIQPETQHALSKVFGSTPMNKQLRPDLNEIPEPTVEFY
                     GKAYSTQLKKDFVREVMMQ"
     gene            3103..4449
                     /locus_tag="RH033_003668"
                     /pseudo
     CDS             3103..4449
                     /locus_tag="RH033_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229742.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
     gene            4442..5674
                     /locus_tag="RH033_003669"
     CDS             4442..5674
                     /locus_tag="RH033_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001486368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDV2992795.1"
                     /translation="MSNAVSFLKYGDVAEFRNGLNFSKDSHGKGCKFIGVADFKDNFT
                     PQWKLLGEINPFGVAKREDYLEPGDIIFVRSNGNKALVGRSLYIDLNEKSLYSGFCIR
                     ARLKTDDFLPLFLAYYTRTNFFKSAITSVAGTNINNLNQDILGNIMIPHYSIGIQKSI
                     VAVLTSIDEKIAINNRINAELEAMAKTLYDYWFVQFDFPDANGKPYKTSGGRMEYNAT
                     LKREIPAGWAVNTLSQIANITMGQSPAGESYNEDGIGTLFFQGSTDFGWLFPTPRQYT
                     TSPTRMAKKGDILLSVRAPVGDMNIANADCCIGRGLAALNSKSRSDGFLFYVMKYFKQ
                     VFERRNAEGTTFGSMTKDDLHSLQVVCPEPGLLKRYDDIVSEYNKMIFTRSLENQDLI
                     KLRDWLLPILMNGQVKIK"
     gene            complement(5921..6067)
                     /locus_tag="RH033_003670"
     CDS             complement(5921..6067)
                     /locus_tag="RH033_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001486734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HDV2992796.1"
                     /translation="MFSKSATELRPKLPALLEDAAMLYLRIGTCRLNNMAPEKWLRYV
                     IEHI"
     gene            6736..7656
                     /locus_tag="RH033_003671"
     CDS             6736..7656
                     /locus_tag="RH033_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HDV2992797.1"
                     /translation="MSTRVWVLGDAVVDLLPESQGRLLQCPGGAPANVAVGIARLGGK
                     SAFIGKVGDDPFGRFMYQTLSTENVDTRYMSLDPQHRTSIVAVGLDEQGERNFTFMVR
                     PSADLFLQPDGLPAFGPGEWLHLCSIALSAEPSRSTAFLAMEKIRQAGGNISFDPNIR
                     SDLWQSEALLRKYLDRALSLANIAKLSEEELLFISGESQVQQGAYSLVQRYSLTLLLI
                     TQGKNGVLVYFQGQFIHYPAKPVSVVDTTGAGDAFVAGLLAGLADSGIPTNTRQLERI
                     IVQAQICGALATTAKGAMTALPRQHDLPSQ"
     gene            7824..9341
                     /locus_tag="RH033_003672"
     CDS             7824..9341
                     /locus_tag="RH033_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HDV2992798.1"
                     /translation="MYKKTTLAVLVALLTDATTVHAQTDISSIESRLAALEQRLKNAE
                     SRAQAAEARAKTAELQVQKLAETQQQNQLTTQEVAQRTVQLEQKSAENSGFEFHGYAR
                     SGLLMNDAASSSKSGPYLTPAGETGGAVGRLGNEADTYVELNVEHKQTLDNGATTRFK
                     AMLADGQRDYNDWTGGSSNLNIRQAFAELGALPSFTGAFKDSTVWAGKRFDRDNFDIH
                     WLDSDVVFLAGTGGGIYDVKWNDTFRSNFSLYGRNFGDLDDIDNNVQNYILTMNHYAG
                     PFQLMVSGLRAKDNDDRKDANGDLIQTDAANTGVHALVGLHNDTFYGLREGTAKTALL
                     YGHGLGAEVKGIGSDGALLSEANTWRFASYGTTPLGSGWYVAPAILAQSSKDRYVKGD
                     SYEWVTFNTRLIKEVTQNFALAFEGSYQYMDLKPKGYQNHNAVNGSFYKLTFAPTLKA
                     NDINNFFSRPELRLFATWMDWSSKLDDFASNDAFGSSGFNTGGEWNFGVQMETWF"
     gene            9402..10772
                     /locus_tag="RH033_003673"
     CDS             9402..10772
                     /locus_tag="RH033_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
                     /protein_id="HDV2992799.1"
                     /translation="MDFDKIAQSLLPLLGGKENIASAAHCATRLRLVLVDDTLADQHA
                     IGQIDGVKGCFRNSGQMQIIFGTGVVNKVYAAFIQVAGISESSKADIARLAAQKLNPF
                     QRIARLLSNIFVPIIPAIVASGLLMGLLGMVKTYGWVNTDNAIYILLDMCSSAAFIIL
                     PILIGFTAAREFGGNPYLGATLGGILTHPALTNAWGVAAGFQTMNFFGFEIAMIGYQG
                     TVFPVLLAVWFMSIVEKQLRRFIPDALDLILTPFLTVVISGFIALLIIGPAGRALGDG
                     ISFVLSTLIAHAGWLAGLLFGGLYSAIVITGIHHSFHAIEAGLLGNPAIGVNFLLPIW
                     AMANVAQGGACLAVWFKTKDTKIKAITLPSAFSAMLGITEAAIFGINLRFVKPFIAAL
                     IGGAAGGAWVVSVHVYMTAVGLTAIPGMAIVQPTSLVNYIIGMVIAFAVAFSLSLLFK
                     YKTDEE"
     gene            10772..12181
                     /locus_tag="RH033_003674"
     CDS             10772..12181
                     /locus_tag="RH033_003674"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDV2992800.1"
                     /translation="MSMPSQMPAILQAVMKGQSKALADAHYPCWHLAPVTGLMNDPNG
                     FCWSGGRYHLFYQWNPLACDHKYKCWGHWSSTDLLHWQHEPLALMPDKEYDRNGCYSG
                     SAVNNQGVLTLCYTGNVKFDDGSRTAWQCLATENNQGGFDKLGPVISLPDGYTGHVRD
                     PKVWKHNSQWYMVLGAQDKEKRGKVLLYSSVDLNTWSFHGEIAGNGLNEIDNAGYMWE
                     CPDLFALDGEYILLCCPQGMVREHERYLNTYPCAWLHGQFDYETGKFMHGAFSELDAG
                     FEFYAPQTMEAPDGRRLLVGWMGGPDGEEMLQPTRKHHWQHQMTCFRELSFQKGKLFQ
                     MPIRELAQLREAEHFWQGKADHAPHVEIERLEMDIIPSGELYLNFGNALALHLNDDGI
                     QLQRRSLAGQEKLTRYWRGSVTSLKILCDSSSVEIFINNGEGVMSNRYFPHHPASLIL
                     QGESDVTLRYWSLRACMVE"
     gene            12207..13214
                     /locus_tag="RH033_003675"
     CDS             12207..13214
                     /locus_tag="RH033_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV2992801.1"
                     /translation="MRKTKRVTIKDIAELAGVSKATASLVLNGRGKELRVAQETRERV
                     LSIAQQQHYQPSIHARSLRDNRSHTIGLVVPEITNYGFAVFSHELETLCREAGVQLLI
                     SCTDENPSQETMVVNNMIARQVDGLIVASSMLHDNDYQKLSEQLPVVLFDRHMNGSTL
                     PLVITDSVSPTAALVADIARSHPDEFYFLGGQPRLSPTRDRLEGFTQGLQQAGVTLQP
                     EWIIHGNYHPSSGYEMFAALCARLGRPPKALFTAACGLLEGVLRYMSQYNLLDSTIHL
                     ASFDDHYLYDSLSVRIDTIQQDNRQLAFHCFELISQLIEGETPSPLQRYLPASLQKRY
                     R"
     gene            complement(13766..14240)
                     /locus_tag="RH033_003676"
                     /pseudo
     CDS             complement(13766..14240)
                     /locus_tag="RH033_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
     gene            14559..15296
                     /locus_tag="RH033_003677"
     CDS             14559..15296
                     /locus_tag="RH033_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HDV2992802.1"
                     /translation="MKKVIAVSALAMAGMFSAQTLADESKTGFYVTGKAGASVMSLSE
                     QRFVDGEGAWADKYKGGDKSDTVFGAGLAVGYDFYQHYNVPVRTEVEFYGRGNAESKY
                     RLSYWESVGGAEFDDAQNKLSVNTLMLNAYYDFRNSSAFTPWISAGLGYARVHHKTSY
                     IYTDNSPAGSEVYSASASKYENNLAWSLGVGVKYDVTQDFSLDLSYRYLDAGDSTLTY
                     KDEDGAKYKSSVDVRSNEFMLGATYNF"
BASE COUNT         4009 a         3447 c         3670 g         4275 t
ORIGIN      
        1 ttaagtgata ttggttgtct ggagattcag ggggccagtc tagtacccat gcccccagtg
       61 tataggaacc gacattcata gcttcactgg attcccggta tgagtaactt ttattaacga
      121 taagctaggc tatttgtgca ttggtgttga ggtgagaata gcaccagtca ggtgagcaga
      181 ttataatgtt acgagactta agcacctctg tagctcccat ggtctgatag tttctcccgt
      241 ataccgctca tgcaactccc gcattaaaat atactgttct ttccggataa ttacgttaaa
      301 atatacacct gcgtgcatct gtgtcagact ccgttagata ccagcgtaca ttgtaaacat
      361 gccggatgca cgcaatacaa taatcggtct tttcggagtt atagtcgcta ggctggcatc
      421 ccgtaaatat gtatcaggta gcaattcagg aagcgaacaa aagcgcctgg agaaggttat
      481 ctctgataat aatttacatg aaatgtctcc tttattcttc gcgaataagg ccttcagtgt
      541 tctccccttt tcagcaattc ttaccgtagt aatcactcct gcctgtgtat aacacgagaa
      601 ttgccagagt ttaacgtttc ggtatcgtta tcctgcatga tgttgaacag aaccgggcgt
      661 gaattctgcg ccagaatttt ctttcgagga acattccggg ttatctgtag tacaggagga
      721 gattttctcc tatttcaaat aaaattcaat gacaggtgaa tatttaaata taaatgcatc
      781 gcttcaacag agaaatcgga attacgcccg gaaccaggcc tgaatgaata atggaggagg
      841 taaaataata ttattgatga aactatcaaa tcaagtcccc cttctccttc cccagcttaa
      901 atatacttac tttttcactg gataatatgt tattgaatta caaagttgat cctgatatac
      961 aggatttccg gctcttgcat ccagtgataa ctttatatag tcctgcaaag gcgaacttcg
     1021 taaaagtata cttttctgtt gtaagcattg aggtatcgaa aatgagtgag aataccggaa
     1081 aatcccatga aaataaaatg gttattattg actgcgggga atatgatgaa taccttcgta
     1141 acgcgtggtt ccgaggtttt cgggaaggtt atgatatggc gatgaaattt ggattaggct
     1201 gccagcaatg ttatgaaaca ggtgaaaaaa tagcatgcgc aagagctgaa gaatgccgta
     1261 aaaagattaa ggtaataaaa cactgaaacg ggctgtctga tatttctgtg agctaaaaag
     1321 acaccgtaat acaggtatct cacagtaact gaaagatatg cacatgcagc cagggaagat
     1381 taagggtaag ggaggatatc ccttacctga ttgattatgg gatgatacca caggcaattc
     1441 ttgcaccacc gccacctaga ggttcaggat ggtcatcatg attatcacct cctgcgtgga
     1501 gcattagcga acggccatcg agctccttta attcagatag tcttggcgca agcaccgggt
     1561 aggttgcttt tccgtcatga gtgacataaa gcgcagggag atcacctagg tggccatcag
     1621 gagaccatgg accgagatgc ttccctgttt tttccgggtc aaagtgacca cctgcagata
     1681 aagcagcgac agtttttcca tctttcaggg caggagcaca acttgcgttt tcatgtacat
     1741 gaaaaccatg aatgccggcg ggtaaggagt gaagatcagg ggtgaacagc agcccgtagg
     1801 gtgtcttctg gatggttatt ttaccaatat ttcgctcttt tccgtcacta tcaacaagat
     1861 tcattgttac atctttggtg gctgcatatc cacaggatga tgtagccaga atcacaaagg
     1921 caataagttt acgtttcata aaccctctgt taacaggtta aagatttact caataactgt
     1981 tgcgaaataa cagtcagcca gatttcaggg ggatggctgt gcgcgaaatg aagcttactg
     2041 catcatgacc tcacgaacga aatctttttt gagctgtgta ctgtacgctt tgccataaaa
     2101 ctccaccgtg ggctccggga tctcattcag atccggacgg agctgtttgt tcatcggcgt
     2161 ggagccgaag actttactca gtgcatgttg cgtttcaggc tgaatcagcc agttgaggaa
     2221 taagcgtgat gccgccggat gtgggctgtt ggcggcaatg gcagcgatgt taccgccgcc
     2281 aggcatgcca aactccggta cgtaaaattt cagccgcgaa ttgatagccc cggttttttg
     2341 tagtccgaca agatggtctt cccaggatgg tgtcagcatc agctcgccgt cattcaggcg
     2401 tgtcagacta tcagcaccgg aggcagtgcg ggtaattttg tccttattag cagcaaacca
     2461 ctgccagcct ttctgccagt ttttcaccac tgccgggtca acgctctccg cttcgctgtt
     2521 aaacgtgtcg ccctgagtag tgacgatgcg ctggataaag gcttcccccg cgccgccgtt
     2581 gttcgggtcg ttatagccga actttttcgg gttggcgtcg ataaagctct gcaactgctc
     2641 cagcgtttgt ggcagcgccc ggtcacccat ctgctgcggg tcgtaagcaa atccggtctg
     2701 gttgccccag aacgccacgg cataaccctg ggtgtcaacg ttctgaatct ggtgaatggc
     2761 gtcgccatag cccggcaaat cgtccacctt cgccagcgct ttcgctccca ttgccactgg
     2821 cagttgcgca acagacaagg cgataacatc tattttaccc ctttttttcc ccgcttctgc
     2881 cagcaacttg ttcacgtttc cgtcgatatt cccttccggg atgcgcacct taacgccgta
     2941 ctgctgctca aaatccttca caaagctgcg ccactgcggc tgtaagtacc agacgttaaa
     3001 ggtcacgctg ccctcttttt tcgcttgggc aatcaggtcg gggctatccg ccgccgttgc
     3061 cagcgagctg cacagcaggc cgcccgccag aatcatcact gttttattcg ttacttgtat
     3121 aaccgacaaa acgcagcgga ctttgccaga acttttgacg acaccctgat ggacatcgcc
     3181 gccaccaaca acgacgtctt cgcggtcaaa accgacggcg gcgcgaaagt ggtgctgttt
     3241 gaacgcctca gccagtacat cgccgacgaa tcaaagcgcg atgacttctg ccgcgccatt
     3301 atcaacaaac tggccgattt cagcttcgaa cgcatcttca cgcagaaatt cgacttctac
     3361 gccactatct ttgaatacct gatcaaagac tacaacagca actccggcgg caaatacgcg
     3421 gaatactaca cgccccacgc cgtggcgcgc atcatggcgg aaatcctggt gccgaaagca
     3481 caacagggcg tagtgcgcaa cgtaagctgt tacgacccgt ccgccggttc tggcacgctg
     3541 ctgatgaacg tggcgcacgc catcggggaa gatcgttgca gcatctttgc gcaggacatc
     3601 tcgcagaaat cctccagcct gctgcgcctg aacctgatcc tcaacaattt ggtgcattcg
     3661 atccctaacg ttatccaggg caacaccatt ctgcatccgt tccataagga cggcagcgcg
     3721 ctgaaacgct ttgactacat cgtctccaac ccgccgttta agatggattt cagcgacttt
     3781 cgcgacgatc tcgacagcaa ggaaaaccag ctacgtttct ttgccgggat accgaaagtc
     3841 aaagccaaag ccagggagaa aatggagatt taccagttgt tcctgcaaca catcattttc
     3901 tcgctgaaac cggatgggaa ggcggcggtc gtggtgccga ctggctttat cactgcgcaa
     3961 tcgggtattg ataaaggcat tcgtgaacat ctggtgaaaa ataagatgct ggcgggcgtg
     4021 gtttccatgc cgtcaaatat cttcgccacc accggcacca acgtgtcgat cctgtttatt
     4081 gacgccagta acaaagagaa agtggtgctg atcgacgcct cgaatttagg tgcaaaagtc
     4141 aaagacggca aaaaccagaa aacggtactg accgaagaag aagagcagcg catctgcgaa
     4201 gtgttcaaca acaagtggag cgaagaggat ttctcggtgg tggtcagcta tgacgacatc
     4261 gccgggaaaa attactcgtt cagcgccggg cagtattttg atgtaaaaat tgaatacgcc
     4321 gatatgacgc cggaacagtt tgccgagaag atgaaaggat ttactgacaa tctggagagt
     4381 ttgtttagcc agtcgctcga gctagaagcg gagattaaaa tgcagatggc ggggctgaaa
     4441 tatgagtaat gctgtttctt ttctgaaata tggtgatgta gctgaatttc gtaatggttt
     4501 aaatttcagc aaagatagcc acggtaaagg gtgcaaattc attggtgtag ctgattttaa
     4561 agataatttc actcctcaat ggaaattatt gggtgaaatt aatccatttg gagttgccaa
     4621 aagggaagac taccttgagc caggcgatat tatttttgtc cgttctaacg gcaacaaggc
     4681 cttagttggg cgaagccttt atatagatct taatgaaaaa tcgttgtact ctggtttttg
     4741 tatccgagcc agattaaaaa cagatgattt tctccctctg tttttggctt actatacacg
     4801 aactaatttt tttaaatctg ctatcacgag tgtggcgggg acaaatatta ataatttgaa
     4861 tcaagatatt cttggtaata tcatgattcc acactattca ataggcattc agaaatcaat
     4921 agtcgctgta ttgacatcta ttgacgaaaa aatcgctatc aacaatcgca tcaacgccga
     4981 actggaagcg atggcgaaaa cgctgtatga ctactggttt gtacagtttg atttcccgga
     5041 tgccaacgga aaaccgtata aaacttctgg cgggaggatg gagtataacg ccacgctgaa
     5101 acgggaaatt ccggcggggt gggcggttaa cacgctgtct caaatagcga atatcacaat
     5161 ggggcagtct ccagccgggg agtcttataa tgaagatgga atcggaacac tattttttca
     5221 gggttcgacg gattttggtt ggcttttccc tactcctcgc cagtacacaa catcacctac
     5281 gagaatggcg aaaaaaggtg atattttact cagtgttcgt gcgcctgttg gggatatgaa
     5341 tattgccaat gccgattgtt gtattgggcg agggcttgcg gcattaaata gcaaaagccg
     5401 ctcagatgga tttttattct acgtgatgaa gtatttcaaa caggtatttg agcgtcgaaa
     5461 tgcggaagga acaacatttg gctccatgac gaaagatgat ttacattcac tgcaagtcgt
     5521 ttgcccagaa cccggattac tgaaacgata tgacgatatt gtttcagaat acaataaaat
     5581 gatttttacg cgtagccttg aaaatcagga tctaatcaaa ctgcgtgact ggctcctccc
     5641 tattcttatg aacggacagg tcaagattaa ataaccccca acgtattttt cgaagcgcag
     5701 tccaagtttg aactgcgctt cacttccccc aacaaaaaac ctgccatcca ttactggtag
     5761 tgattcctct cacattttcc atgtattaat tattattgtt actactacac ccaaacgacc
     5821 tataatcgta agcggctcgc cagaaccgta ttgatattta ctgagcgctc agatcaactt
     5881 tccagggtaa cagatcgcgt atccagtttg cctgtcattc ctagatatgt tcaatgacgt
     5941 aacgcagcca tttttctgga gccatattgt tcagacggca tgtaccaatc cgcaggtaca
     6001 gcatcgccgc atcctcaagt aatgccggta atttggggcg taattctgtt gcgcttttac
     6061 tgaacacaat gccgtattca gtaagaagtc caagaacatg atctatcagt tgtgtccttc
     6121 catcgagatc aaccgatgca ttttcaaaaa agcctggacg ttctgtaggt ccagactttt
     6181 tattggtacc agtcgtatag ttctatattg ataggtttca gctattgctg tgcgtcgtta
     6241 taatcatttt tctgacgcca ttgataagat ttaacatgtt gtgctggatg caatattaca
     6301 tcatagttga gttcccttat ttcctgtggg cagtgatggg atgtcccgca agccttcatc
     6361 gcaacggtca ttttggctgt tataccagcc agttaagcag ttggttgtga gaaagtttga
     6421 tctttcccgc gtgattgcca cgagcattga tactgaaaag ataaaagttc gttttagcct
     6481 gagcgatgtc ggtaattttc tgttcagtca tggcctttct cttacttttg tggttaaaat
     6541 ttattcgcta tggcgcaatt gaaagcggtc gtgaaggtgg gggaagacta tctcatcact
     6601 taccatattt ccgacatatt ctcacaacaa aataccacgt aactattgat gaatattgtt
     6661 ctctcctgag cagcgtttat gctttaattg ctaaatcgat ttaattagtt tagcaattag
     6721 tatattggag cctttatgag cacaagagta tgggtacttg gtgatgcggt tgttgattta
     6781 ttacccgaaa gccaggggag actactacag tgtcctggcg gggcgcctgc taatgttgca
     6841 gtcggtatcg caaggctggg ggggaaaagt gcctttattg gcaaagttgg cgatgatcct
     6901 ttcggtcgct ttatgtatca gacactgagt acagaaaatg ttgatacacg ttatatgtct
     6961 cttgatcctc aacatcgcac ctcaattgtg gctgtaggac ttgatgagca aggagaaaga
     7021 aactttacct ttatggtacg cccaagtgcc gatctttttt tacaacctga tggccttcct
     7081 gcatttgggc cgggtgaatg gctccatctt tgttccattg cgctcagtgc agaaccttcc
     7141 cgaagtaccg catttctggc tatggagaaa atacgtcagg ctggcggaaa catcagtttt
     7201 gatcccaata tccgcagcga tctctggcag agtgaagcgc tattaaggaa ataccttgat
     7261 cgcgcacttt cgctggcgaa tatcgctaaa ttgtccgaag aagagttgct attcatcagt
     7321 ggcgaaagcc aggttcagca aggcgcatat tcattagtac aacgttattc gttgacttta
     7381 ttgcttatca cacaaggaaa aaatggcgta cttgtgtatt ttcaggggca gtttatccac
     7441 tatcccgcca aacctgtttc tgtcgtcgat acgaccgggg caggagatgc ttttgtcgct
     7501 ggattacttg ccggtctggc tgattctgga ataccaacaa ataccagaca gcttgaacga
     7561 atcattgtac aagctcagat ttgtggtgct ctggcgacca cggctaaagg cgcgatgacc
     7621 gccttacccc gacaacacga tctcccttca caatagtgat cctgctgttg tatctgtaac
     7681 ccgatttgtc gtgcctgtca catttactaa atcggtttag cgaaaataat tgctttggct
     7741 aaaaagtcag ctttattatc aacacctaaa ccggtttagc attttagcaa tttttataat
     7801 tctctgatgg gacgcgacaa attatgtata aaaaaacaac tttggcagtg ttagttgctt
     7861 tgctgaccga tgctacaacg gtacatgcgc aaacggatat tagcagtatc gaatctcgac
     7921 tggcggcgtt ggaacaacgt ttaaaaaatg cggaatcccg cgcccaggcg gcagaagcaa
     7981 gggccaaaac agctgaatta caggttcaga aactggctga aacacaacaa caaaatcagc
     8041 taacaactca agaagtagca cagagaacag ttcagctcga acagaaatcc gcagaaaaca
     8101 gtggttttga gtttcatggc tatgcccgtt ccgggttact gatgaatgat gccgcttcca
     8161 gtagcaaaag tgggccgtat ctgactcccg caggtgaaac tggtggagct gttggccgtc
     8221 tgggaaatga agccgatacc tatgtcgagt taaatgtaga acataaacaa acactggata
     8281 acggtgcgac cacacgcttt aaagcaatgt tggctgacgg acaaagagat tacaacgact
     8341 ggactggcgg ctccagtaac ctgaatatcc gacaggcttt tgccgaactg ggcgcattac
     8401 caagttttac cggagcattc aaagacagta ctgtctgggc tggtaaacgc tttgatcgcg
     8461 acaattttga tattcactgg ttagactccg atgtcgtatt tttagcggga acgggcggcg
     8521 gtatctatga cgtaaaatgg aacgatacat ttcgcagtaa cttttctctc tacggacgta
     8581 atttcggcga tcttgatgat atcgacaata acgttcagaa ttacatcctc accatgaatc
     8641 attatgcagg ccccttccag ttgatggtta gcggattacg ggcaaaagat aatgatgatc
     8701 gaaaagatgc caatggtgat ctcattcaaa ctgatgctgc aaatactggc gtacatgcgt
     8761 tagttggtct gcacaatgac actttctatg gcctgcgtga agggacggca aaaacagcac
     8821 tgctatatgg ccatggcctg ggtgcggaag tcaaagggat tggctccgat ggcgccctgc
     8881 tgtctgaggc gaatacttgg cgcttcgcat cttacggcac aacacctctg ggaagcggtt
     8941 ggtatgtcgc accagcaatt ctcgcacaaa gcagtaaaga tcgttacgtc aaaggcgata
     9001 gctacgaatg ggtgaccttc aatacacgtc tgatcaaaga ggtaacacag aattttgctc
     9061 tggcctttga gggtagctat caatatatgg atctgaaacc aaaggggtat caaaaccaca
     9121 acgccgtaaa cggcagcttc tataaactca cctttgctcc aactctaaaa gctaacgata
     9181 tcaataattt ctttagccgt ccggagcttc gcctgtttgc cacctggatg gactggagca
     9241 gcaaacttga tgattttgcc agcaatgacg ctttcggcag cagtggtttc aatactggcg
     9301 gagagtggaa ttttggtgtc caaatggaaa cctggtttta ataccagaca gcctcacaga
     9361 tgtgaggttt ttactgaaat aacaatgaat tagagggtta tatggatttc gataaaatcg
     9421 cccaatcgtt gcttccttta ttaggtggta aggagaacat cgccagtgca gcacattgcg
     9481 ccactcgcct acggctggta ctggttgacg acacacttgc cgatcaacat gccattggcc
     9541 agattgatgg agtgaaaggt tgttttcgta attcagggca aatgcaaatt atctttggca
     9601 ccggcgtggt taataaagtc tatgctgcct ttattcaggt tgcaggtatt agcgaatcca
     9661 gtaaagcaga tatcgctcga ctcgccgctc aaaaactcaa tccttttcag cgaatagcac
     9721 ggctactttc taatattttt gtccccatca ttccagccat tgttgcttct ggtttattaa
     9781 tggggcttct gggaatggtg aaaacatatg gctgggtgaa taccgataat gcgatttata
     9841 tcctgctgga tatgtgcagt tcagccgcat ttattattct ccccatcctt attggcttta
     9901 ctgctgctcg tgaatttggt ggaaatcctt atcttggcgc gacattagga gggattctga
     9961 ctcacccggc actcacaaat gcctggggcg ttgctgcggg atttcagaca atgaacttct
    10021 tcggttttga aatcgccatg attggctatc agggaacggt tttccccgtg cttctggcag
    10081 tatggtttat gagcatagtg gaaaaacagt tacgcaggtt tatccctgat gctctggatc
    10141 tcattctgac gccatttctg actgtcgtca tttctggctt tatcgctctt ttgattattg
    10201 gtccggcagg acgagcgtta ggtgacggta tctctttcgt tctcagcacg ctgattgcac
    10261 acgcaggctg gttagcaggg ttgttattcg gcgggctata ttcagcgatt gttattacgg
    10321 gtattcatca tagcttccac gcaattgaag caggactttt aggtaaccct gcaatcggtg
    10381 ttaatttcct gctgcctatt tgggcaatgg caaacgttgc gcaaggcggt gcatgtctgg
    10441 cggtatggtt taaaaccaaa gatacaaaaa ttaaagcaat caccctaccc tcggcctttt
    10501 ccgcaatgtt agggattact gaagccgcta tttttggtat caaccttcgt tttgttaaac
    10561 cttttattgc ggccttaatt ggtggcgctg ccggcggtgc ctgggttgtc tctgttcacg
    10621 tttatatgac tgccgttggt ctgaccgcaa tacccggcat ggcaatcgtt cagccaacat
    10681 cgttggttaa ttatattatt ggaatggtga ttgctttcgc tgtcgctttt agcctctctt
    10741 tattattcaa atacaaaacg gacgaggagt aatgtcgatg ccttcacaaa tgcccgcgat
    10801 tttacaggca gtgatgaaag gtcagtccaa agcactggca gatgcccact atccttgttg
    10861 gcatctcgct cccgtcaccg gactaatgaa cgatccaaat ggcttttgct ggtctggagg
    10921 ccgctatcat ctgttttatc agtggaatcc actggcttgt gatcataagt acaaatgttg
    10981 ggggcactgg agttcgacgg atttattgca ctggcagcat gaaccattag cgctaatgcc
    11041 cgataaagag tacgaccgta atggttgtta ctcaggaagc gcggttaata atcagggcgt
    11101 tctcacactt tgctataccg gtaatgttaa gtttgatgat ggcagcagaa cggcatggca
    11161 atgtctggcc accgaaaata accagggcgg gtttgataaa ctaggccctg tcatatcgtt
    11221 acctgatggt tacaccggac atgttcgtga tccaaaagtc tggaagcaca acagccaatg
    11281 gtacatggtg cttggcgcgc aagataaaga gaaacggggg aaagtgctgc tgtattcctc
    11341 tgtcgatctg aatacctggt cttttcatgg agaaattgct ggtaacggtc tgaatgaaat
    11401 agataacgca ggatacatgt gggaatgtcc tgatcttttc gcccttgatg gtgaatacat
    11461 tctcctctgt tgcccccagg gaatggtgcg cgaacatgag cgctatctga atacttatcc
    11521 ctgcgcctgg ctacatggac agtttgatta cgagacaggc aaatttatgc atggcgcttt
    11581 ctcagagctg gatgcgggat ttgaattcta tgcaccacag acgatggaag ccccggacgg
    11641 gcgacgattg ctggtcggct ggatgggggg tcctgatggc gaagagatgt tgcagccaac
    11701 cagaaagcat cattggcagc atcaaatgac ctgttttcgc gaactctcat tccagaaggg
    11761 aaaattgttt cagatgccaa tcagggaact cgcgcaactg cgtgaagctg aacacttctg
    11821 gcaaggaaaa gcggatcatg cgccgcatgt cgaaattgaa cgcctggaaa tggacatcat
    11881 tccgtcgggt gagctgtatc tgaattttgg caatgcgctg gcactgcatc ttaatgacga
    11941 tggtattcaa ctacagagga gaagccttgc agggcaagag aaactaactc gttactggcg
    12001 aggaagcgta acgtcactga agattctctg tgacagttcc agcgtagaga tattcatcaa
    12061 taatggtgaa ggtgtaatga gtaatcgtta ttttccccat catccagctt cgctgatatt
    12121 acagggtgag tctgacgtca cgttacgcta ctggtcgcta cgcgcctgca tggtagaatg
    12181 agggttttga attacggatc ctcgtcgtga gaaaaaccaa acgcgttact attaaagaca
    12241 ttgctgaact ggcgggagtg tcgaaagcaa ccgccagtct ggttctcaat ggtcgtggca
    12301 aagagttacg tgttgcgcag gaaacgcgtg agcgtgtgct gagtattgcc caacagcagc
    12361 attatcagcc cagcattcat gcccgctcat tacgggacaa ccgcagtcat accattggtc
    12421 tcgtcgttcc tgaaatcact aactacggat ttgcagtatt ttcccatgaa ctggaaacat
    12481 tgtgtcggga agcgggcgtc caactcctca tatcctgtac tgatgaaaat ccttcacagg
    12541 aaacaatggt ggtgaacaat atgattgccc gccaggttga tgggttaatc gtcgcatcca
    12601 gtatgctgca tgataatgac tatcaaaaac ttagcgagca attacctgtt gtgttgttcg
    12661 atcgccacat gaacggcagc acattgccat tggtcattac tgactctgtt tctcccaccg
    12721 ccgctctggt ggcagatatt gcacggagtc acccggatga attctatttt ctggggggac
    12781 aaccccgttt gtcaccgact cgcgatcgac tggaaggatt tacacaaggc ttgcagcaag
    12841 ccggagtaac gctacaacct gaatggatta ttcacggtaa ttatcatccg agctcaggct
    12901 atgaaatgtt tgccgcgcta tgcgctcgtt taggacgtcc tcctaaagcc ttattcactg
    12961 ccgcttgtgg attactggaa ggcgttctgc gctatatgag ccaatataat ttactcgaca
    13021 gtacaatcca cctggcgagt tttgatgatc attatttgta tgattcactt tcggtaagaa
    13081 tcgacacaat acaacaggat aatcgccaac ttgcttttca ctgctttgag cttatttcgc
    13141 aactgattga aggtgaaacg ccatccccat tacagcgtta tcttcctgcc agtttacaaa
    13201 aacgctatag ataatttatt aattatctta tgtagctgag tattaggtga caaatgatca
    13261 gtctttccca cgacaattgt atcgttctga tgaactcata ttgacgcatt tttcgggcgt
    13321 atcagtcccc ggtgttacga atggtgcata cgaacgatcg cagtttgacg atggttgatt
    13381 tattttgttg cgcaggggca atcgcctgct aggtaccgaa ggctggctct gaagtactga
    13441 gtcgggtgat cgcgtctggc cgctggctgc gaattactga cacgggattg gtggcaggtg
    13501 atgaaatcct gtcccggaaa tggctccgga ttgattcaag ttctgacccg ctgtcagccc
    13561 gcgtggttgc tgctctggct catttcctgt cgtcagtttt ctcatgcccg gctgcgggtt
    13621 ttcggactga atgataatat ctgacgacag gagtcgctca tctatcgaag gtaaatctcc
    13681 ctgaaagtaa tagttctggc agggggaaca gaactgtaat cattgtaccg taatttgagt
    13741 tggctacctt tgctacatga aaactttatt cactttgttc atgttgattg gggttttcaa
    13801 tgaggaaagt ttccagggta caatctccat taacctgttt ccgcaaagca agtggcattt
    13861 taccgttacc agacccacgt cctggtttcg ccgttctcat cctcaaagat atatttcgct
    13921 tttctcacac cgcctttggg ttttcgtttg gtttccgttg tccctgcaat agcagaaact
    13981 ggaccctgcc attcttccgg gtcaagcccc acagaaacga tgtactcaaa tacctgttga
    14041 catttagttt ccagttcctt acgttcagcc gcttttcgtt cagcatcttc acggcgttct
    14101 tcaataacag tgttgagctt ttcctgcatg tcgagtaatt gctcaaaatc aagttcgcgt
    14161 acatacgtgc gtagtgaacg aatattcgtc attgtccgac tgatgatgct gtattcttct
    14221 tctttagtca gtttgcacat aattcccctc tcttttgcaa taaacgtgtt ttctctggtg
    14281 ggttgattac ccagtctttt ttactgtgtc tgacattttg tactgtggtg cagttgtaat
    14341 gcaaaccttc cgggcttttg ggacacagca tttcagtccg ggccccatca ttaccatttt
    14401 gttgattcga aacgaaaacc ctcaccaata tcgatcgatt ttaccgatcg atatgatcac
    14461 atcgatcggt tgtaaccatt ttaccctgtc ttttgcatgg ttttaaaata ggcaaccctc
    14521 tttgtcgggt agggcaagaa ccaacggaga tgattgaaat gaaaaaggtt attgcggttt
    14581 cagcgcttgc aatggcaggc atgttttcgg cccagactct ggctgatgag agcaaaacag
    14641 gcttttatgt gaccggtaaa gcaggtgctt ctgttatgtc actttcagag caacgtttcg
    14701 ttgatggtga aggggcatgg gcggataaat ataaaggcgg tgacaaaagt gatacggttt
    14761 ttggcgcagg ccttgcggtg ggatacgatt tttatcagca ctacaatgtg ccggtgcgta
    14821 ctgaagtgga gttttatggc cgcggtaacg ctgaatcaaa atatcgtctg agttattggg
    14881 aaagtgtcgg tggtgccgag tttgatgatg cacagaacaa gctcagtgta aacacgctga
    14941 tgcttaatgc gtattatgat ttcagaaaca gcagtgcatt cacgccatgg atatctgctg
    15001 gtctgggtta tgctcgggtt catcataaaa cttcgtacat atataccgat aacagtccgg
    15061 caggcagtga agtatattca gcttcagcgt caaaatacga aaataacctg gcatggagcc
    15121 ttggtgtcgg tgttaaatac gatgtgacgc aggatttcag ccttgacctc agttaccggt
    15181 atctggatgc aggggattcc actctgactt ataaagatga ggatggcgct aaatataaat
    15241 cctctgttga tgtcagaagt aatgagttta tgttaggtgc aacatataac ttctgatggc
    15301 catttctgcc tgcgctttgg agcacagtaa agtgcaggta tgacaaatca ataacagata
    15361 tcctgatgat attctccctc tggcaactaa taatttcaga t
//
LOCUS       DAPHCV010000109        14988 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.109, whole genome shotgun sequence.
ACCESSION   DAPHCV010000109 DAPHCV010000000
VERSION     DAPHCV010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.109"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(92..2371)
                     /gene="maeB"
                     /locus_tag="RH033_003678"
     CDS             complement(92..2371)
                     /gene="maeB"
                     /locus_tag="RH033_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HDV2992803.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQSDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2660..3610
                     /gene="tal"
                     /locus_tag="RH033_003679"
     CDS             2660..3610
                     /gene="tal"
                     /locus_tag="RH033_003679"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDV2992804.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3630..5633
                     /gene="tkt"
                     /locus_tag="RH033_003680"
     CDS             3630..5633
                     /gene="tkt"
                     /locus_tag="RH033_003680"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDV2992805.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKYAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5710..6753)
                     /locus_tag="RH033_003681"
     CDS             complement(5710..6753)
                     /locus_tag="RH033_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HDV2992806.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRVFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSREERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(6879..7454)
                     /gene="nudK"
                     /locus_tag="RH033_003682"
     CDS             complement(6879..7454)
                     /gene="nudK"
                     /locus_tag="RH033_003682"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HDV2992807.1"
                     /translation="MTQQITLVKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDSQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(7522..9501)
                     /gene="aegA"
                     /locus_tag="RH033_003683"
     CDS             complement(7522..9501)
                     /gene="aegA"
                     /locus_tag="RH033_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HDV2992808.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVEVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9707..11407
                     /gene="narQ"
                     /locus_tag="RH033_003684"
     CDS             9707..11407
                     /gene="narQ"
                     /locus_tag="RH033_003684"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HDV2992809.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGDNWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            11571..14684
                     /gene="acrD"
                     /locus_tag="RH033_003685"
     CDS             11571..14684
                     /gene="acrD"
                     /locus_tag="RH033_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HDV2992810.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGMAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14783..14842)
                     /gene="ypfM"
                     /locus_tag="RH033_003686"
     CDS             complement(14783..14842)
                     /gene="ypfM"
                     /locus_tag="RH033_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HDV2992811.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         3516 a         3962 c         3911 g         3599 t
ORIGIN      
        1 attcgcaaac tcagtaataa actttactaa tatattgcag ctaaaccgct taccctgaat
       61 attcagggta agcgtgagag ttaaaaaaga attacagcgg ttgggtttgc gcttcaacca
      121 cggccagcgc caccatgttg acgatacgac gtaccgatgc gatcggcgtt aacacgtgaa
      181 ccggtttcgc cacgcccatc agcaccggac cgacagtcac accttccgag ctggaaacgc
      241 gcagtaagtt gtaactaatg cgggcagctt ccatgttcgg catcaccaga atattagcgg
      301 aacctttcaa agggctgtcc ggcatacggt cgttgcgaat cgcttccacc agcgctgcat
      361 cgccgtgcat ttcaccatca atcatcagtt ctggcgcacg ttccctgacc agttccagcg
      421 cctgacgcat tttgctcgac gacgggcagt cagaagaacc aaagttggag tgcgacaaca
      481 gagccacacg cggctcaata ccaaaacgac gaacagtttc tgccgccatc aaggtgatct
      541 ccgccagctc ttctgcatca ggctcatcat tgacgtaggt atcggcaata aaggtgttac
      601 cactcggcaa cagcagcgcg ttcattgcac ctgcggtgtg aacgccatcg cgataaccaa
      661 agacattttt caccacgcta aagtgttcat gataatcgcc caccgtaccg caaatcattg
      721 catcggcttc gccacgctga accatgatcg cgccgatcac tgtcgggtta ctgatcagcg
      781 cacgctgcgc ctgctcctga gtgacaccgc gacgcttcat gatctggaag tattcggtcc
      841 agtactcttt aaagcgcgga tcggattcgt tattgacgat ctcaaaatca acgcccgcct
      901 tgatctgcaa gcccagtttc tgaatgcgca tttcgatcac gttcggacga ccaataagga
      961 tcggtttcgc cagtcccagc gttaccagtt cctgagtggc atgcagaaca cgcgcctctt
     1021 ccccttccgg cagaactacg cgcttcggcg ctttgcgagc ctgggagaaa atcggcttca
     1081 taaacaggtt agttttgtaa acgaactctg tcagcttgtc gatgtagacg tcgaaatcag
     1141 caatcggacg cgtcgccacg cccgactcca tcgccgcttt cgccaccgca ggagcgatct
     1201 taacgatcaa gcgcggatca aacggttttg gaatgatgta ttccggacca aagctcagat
     1261 cctgatcgcc atacgctgaa gccaccactt cactctgttc cgcatgggcc agttctgcaa
     1321 tcgcgcgtac cgccgccagt ttcatctctt cgttgatggc ggttgcgcca acgtccagcg
     1381 cgccacggaa gataaacggg aagcaaagga cgttgttcac ctggttcggg tagtcggaac
     1441 ggccggtaca gatgatggca tccggacgca cttctttcgc cagcggcggc agaatttccg
     1501 gttccgggtt cgccagcgcc aggatcattg gcgcacgggc cattttcttc accatttcct
     1561 gggtcagcac tttcgggccg gaacagccga ggaaaatatc cgcgccttca atcacatcat
     1621 cgagggtacg tttaccgtca tccaccaccg catatgcggc tttggtttcc gccatgtttg
     1681 gttcacggcc ctgatagata acgccttttg aatcgcaaac cacgatgtta tgtttttgca
     1741 gacccagcgc taccagcagg ttcatacagg cgattgctgc ggcacccgcg ccagaaacca
     1801 ccatccgcac gtcggagatg tttttctcca ccacgcgcaa gccgttgagg atggcggcag
     1861 tgctgataat tgccgtgccg tgctgatcgt cgtggaatac cggaatattc atccgctcgc
     1921 gcagtttctg ttcaatatag aaacattctg gcgctttaat gtcttcgagg ttgatgccgc
     1981 caaaggtcgg ttcgagcgca gcgacgactt caataaattt gtccgggtcg agttcatcaa
     2041 cttctatgtc aaatacatca atcccggcga atttcttaaa cagaacgccc ttgccttcca
     2101 tcaccggttt gcctgccagc gcgccaatgt tgcctaaccc cagcaccgcc gtaccgttag
     2161 agatcaccgc taccaggtta ccgcgcgcgg tatatttgta ggcttttaac gggtcttttt
     2221 cgatttcaag acaaggtgcg gcaacgcctg gtgagtaggc cagcgccaga tcgctctgcg
     2281 ttgccagagg cttggttgga gaaacctgga ttttccctgg aactggaaat tcatggaaat
     2341 caagtgcact ttgttttaac tggtcatcca tttggttgtt cctttcacgt aacgttcaca
     2401 aataaagtgt gtgggcaaca gcccctgtcc acaacgtggc gcacattatt accctgccgg
     2461 agtctacaga ctttgagcaa gtccaaactc tcaccattaa tataatgttt tggtaataat
     2521 cctataacac tgatgttacc tgcttaatcc agcaatacca tgcctgtctg ctatgctttt
     2581 ttgatgcgtt tagcgaaatt tctcagaagt gtgaattaac gcactcatct aacactttac
     2641 ttttcaagga gtatttccta tgaacgagtt agacggcatc aaacagttca ccactgtcgt
     2701 ggcagacagc ggcgatattg agtccattcg ccattatcat ccccaggatg ccaccaccaa
     2761 tccttcgctg ttactcaagg ctgccggatt atcacaatat gagcatttaa tagacgatgc
     2821 tatcgcctgg ggtaaaaaaa atggcaagac ccaggaacaa caggtggtcg cagcgtgtga
     2881 caaactggcg gtcaatttcg gtgctgaaat tctcaaaatc gtacccggtc gcgtgtcaac
     2941 agaagttgat gcacgcctct cttttgataa agaaaagagt attgagaagg cgcgccatct
     3001 ggtggacttg tatcagcaac aaggcgttga gaaatcacgc attttgatca agctggcttc
     3061 aacctgggaa ggaattcgcg cggcagaaga gctggaaaaa gaaggtatta actgcaacct
     3121 gacgctgctg ttttcttttg cccaggcgcg ggcctgtgca gaagcgggcg tttttctgat
     3181 ttcgccgttt gtcgggcgta tttatgactg gtatcaggca cgcaagccga tggacccgta
     3241 tgtggtggaa gaagatccag gcgttaaatc agtgcgcaat atctacgact actataagca
     3301 acaccactat gaaaccattg tgatgggcgc gagcttccgt cgcaccgaac aaatcctcgc
     3361 cttaaccggc tgcgaccgac tgactatcgc accgaatttg ctgaaggagc tacaggaaaa
     3421 agtttcgcca gtggtacgta aattaatccc accttctcag acgttcccac gcccagctcc
     3481 catgagcgaa gcggagttcc gttgggagca caatcaggat gcgatggcgg tagaaaaact
     3541 gtctgaaggc attcgtctgt tcgccgttga tcaacgcaaa ctggaagatc ttcttgccgc
     3601 caaactataa accagccacg gagtgttata tgtcccgaaa agaccttgcc aatgcgattc
     3661 gcgcactcag tatggatgcg gtacaaaaag ccaattctgg tcatcccggc gcgccgatgg
     3721 gcatggctga tattgccgaa gtgctgtgga acgattttct taaacataac cctaccgacc
     3781 caacctggta tgatcgcgac cgctttattc tttccaacgg tcacgcgtcg atgctgctct
     3841 acagtttgct gcatctgacc ggttacgacc tgccgctgga agagctgaag aacttccgtc
     3901 agttgcattc aaaaacccca ggtcacccgg agattggtta tacgccaggt gttgaaacca
     3961 ccaccggtcc acttggacaa ggtttggcga acgccgtcgg gctggcgata gcagagcgta
     4021 cactggcggc gcagtttaac cagccggatc atgaaattgt cgatcacttc acctatgtgt
     4081 ttatgggcga cggttgcctg atggaaggta tttcccacga agtctgttcg ctggcgggca
     4141 cgctgggact gggcaagctg attggttttt acgatcacaa cggtatttcc atcgacggtg
     4201 aaaccgaagg ctggtttacc gacgatacgg caaaacgttt tgaagcctat cactggcatg
     4261 tgatccacga gatcgacggt cacgatccgc aggcggtgaa ggaagcgatc cttgaagcgc
     4321 aaagcgtgaa agataagccg tcgctgatta tctgccgtac ggtgattggc tttggatcgc
     4381 cgaataaagc aggtaaagaa gaggcgcacg gcgcaccgct gggtgaagaa gaagtggcat
     4441 tggcacggca aaaactgggc tggcaccatc cgccatttga gatccctaaa gagatttatc
     4501 acgcctggga tgcccgcgaa aaaggcgaaa aagcgcagca gagctggaat gagaagtatg
     4561 cagcctacaa aaaggcccat ccgcaactgg cagaagagtt tacccgtcgg atgagcggtg
     4621 gtttaccgaa ggactgggag aaaacgactc agaaatatat caatgaatta caggcaaatc
     4681 cggcgaaaat cgctacccgt aaggcttcgc aaaatacgct taacgcttac gggccgatgc
     4741 tgcctgagtt gctcggcggt tcggcggatc tggctcccag caacctgacc atctggaaag
     4801 gttctgtttc gctgaaggaa gatccggcgg gcaactacat tcactacggg gtgcgtgaat
     4861 ttggcatgac cgctatcgcc aacggcattg cgcaccacgg tggatttgta ccgtataccg
     4921 cgacgttcct gatgtttgtt gaatacgccc gtaacgccgc gcggatggcg gcactgatga
     4981 aagcgcggca gattatggtt tatacccacg actcaattgg cctgggcgaa gatggtccta
     5041 cgcaccaggc tgttgagcaa ctggccagtt tgcgcttaac gccaaatttc agcacctggc
     5101 gaccgtgcga tcaggtggaa gcagcggtgg gctggaagct ggccgttgag cgccacaacg
     5161 gaccgacggc actgatcctc tccaggcaga atctggccca ggtggaacgt acgccggatc
     5221 aggttaaaga gattgctcgt ggcggttatg tactgaaaga cagcggcggt aagccagata
     5281 ttattttgat tgccaccggt tcagagatgg aaatcaccct gcaagcggca gagaaattag
     5341 cgggagaagg tcgcaatgta cgcgtagttt ccctgccctc gaccgatatt ttcgacgccc
     5401 aggatgagga atatcgggag tcggtgttgc cttctaacgt tgcggctcgc gtggcggtgg
     5461 aagcaggtat tgccgattac tggtacaagt atgttggtct gaaaggggca attgtcggga
     5521 tgacaggtta tggggaatct gctccggcgg ataagctgtt cccgttcttt ggctttaccg
     5581 ccgagaatat tgtggcaaaa gcgcataagg tactgggagt gaaaggtgcc tgatggtgat
     5641 cgccggatgc tgattgtcgg atgtgacgct ggtgcgtctt atccggtcta catgtccccg
     5701 ccattttgtt taacgggtga tccacaacgt gggccaggcg tctggcccat gccagttatc
     5761 gcaggtgggt tctgcggcgt aacgcaccag gcgaaaacgc tgaccgtcaa agcgccagcg
     5821 cgcctgaatg ccacaatcgc ttaatccgcg cccttttgct aaggtcacca gttcacgcga
     5881 tttttcatcg aatgttgcgt tcatcagttc cagttcattg gtctcctgac cgttgttgaa
     5941 cggcaaacgc aaccgtaccg ggcgcgaggc cagcggcttt ttacgcgaca caatccatgc
     6001 caaatcaatg gtgttatagg cccctgcctc acagctaatc atcatcagcg ctttatcatc
     6061 ggtcagcgca gtgacattca cctcacgacg taacggatca agcgaacagc gcagaccatt
     6121 catccgccag tttccataat ccagcaaatc gttacgttct tcgcgtgaga gtggcgtcgg
     6181 cgttgggtta accaccgcga cctctttcag cgcaggcgct ggcggtacgc tgagcggcgg
     6241 ttcgtcccct ttctttatcc acgcggtttc actgccaacg cgcttttgct gagcatcaat
     6301 aaacaacaac gccgctttta agccactcag agaaatggtc tgattgccgt cgcgtaaggt
     6361 gatcgccttc ccttcctgaa tcatctgcaa aaacgcggtg atggttgccg tatcatcggt
     6421 tactaataac catggtgaaa tccgccactt gtcaccactt aacgccaacg gctcgccatc
     6481 taacagcagc cgtggcgcta tctccccttc tgacgcatcc ggcgacttca atccgccgcg
     6541 ctcaatacgt aaaacggcat cggtatgcgc tccggcgctg cggctcaggg tcatcaccag
     6601 tccattatga tcacccgtat tgcgcgccac acaaaaattt tgattattac aggtgacctg
     6661 ccagtcggaa aacacccgtt gcgctggtgc cgcccacacc aaagacgtcg gcaacaaagc
     6721 gaaaaaaaag agaaggaaaa tgcgatagcg catggacggt acgaccccag aagttcaaac
     6781 aaacagcaca agtcgtatct tcgttgcgca ggtagcgcgg ctcaatcgga tttatcggat
     6841 tgagccaata ataaacaacg aaatattatt gttatttttc agtccattaa atgtgacatt
     6901 tgcaaatagt taagcaataa caccgtctta ccgtcacgta tctcgccggt tttaatcatc
     6961 tccagcgcct ggctgaacgg cagctcgagc acttcaatat cttcatcttc gacaccaccg
     7021 cccgcgttag cgcgctggct gtcactgtat tcggcgataa agaagtggat tagctcagtc
     7081 acaccgcctg gcgacatata cagttcaaat aatttgcgca cttcgccaac ttcataaccc
     7141 gtctcttcaa tcgcttcttt acgaatacac acttccggtt cgtcgttatc cagcagcccg
     7201 gcgcaggttt caatcagttg cccgctttca ttgccattaa cccaggtagc gacgcggaac
     7261 tggcgaatca gaaccacggt ctttttcttc gcgttgtaca ggaggatcgt tgcaccatta
     7321 ccgcgatcgt aaacttcacg tttatgacgg ataacttcgc catctttgcg cgtgagatcg
     7381 taagtaatgt tgtgcagggt gaaatagtta tcggagagaa ttttgtcttt aacgagggtg
     7441 atttgttgcg tcataccgac tccacagcgc gaaatgaaca attatcttac gctgtgaagt
     7501 cgggtttgtc tgcgcaggct atcagtgaga tttgactgat tttacgccca gccagtcaat
     7561 gatcccctgt gccgcatgac gtccttctgc cattgcggta accaccagat ccgcaccacg
     7621 cacggcgtca ccaccggcga agattttcgg attcgtggtc tggtaacggt actggctttc
     7681 cacatccgcg atgatgcggc cccatttgtc taccgttaca ccgtgcgact ccagccacgg
     7741 catcccgtgc ggattgaagc caaacgccat aatcaccgca tcggctggca tgacaaattc
     7801 actgccttcc accggcactg gacgccgacg tccctgggcg tccggctcgc caagacgcgt
     7861 acgcaggaaa cgaattccgc aaacgtgacc ttgctcattc agctcaagcg ccaccggctg
     7921 gacgttaaat tcaaagttgg ccccctcttc gcgggcgttc ttcacctctt tcttcgagcc
     7981 tggcatgtta gcttcatcac gacgataagc gcaggtgacg ttactcgcgc cgtggcgcag
     8041 cgcggtacgc acacagtcca tcgcggtgtc gccgccgccc agtaccacga cgttgagtcc
     8101 ggcggtattg ataaacggct cttccggtag ctcttcgagt cccatcacct gtttagtgtt
     8161 ggcgatgagg aacggcagcg cgtcataaac gcccggcgca tcttcattgg gtaaacccgc
     8221 tttcatggaa cggtaagtgc ctacgccaac gaagaccgcg tcgtattgtt ccaaaagcga
     8281 atccaaagag acatctttac ccacttcaca attgagttcg aagtgaatcc ccatcgcgct
     8341 gaagatttcc cgacggcgtg ccagcaggga tttatccagt ttgaaagaag gaatgccgaa
     8401 agtgagcaag ccaccgattt ctggatggcg atcgtacacc gtcacctcta caccattgcg
     8461 ggtcaaaaca tccgcacagg ccagccctgc cggacccgca ccgataatcg ccacccgctt
     8521 gtccactttg gtgacatgac ttaagtcagg acgccaacct ttcgccaacg cctgatctga
     8581 aatgtagcgt tcaatgttgc cgatagttac tgcgccgtgc tcatcgcgaa tagtacaggc
     8641 accttcacac aaacgatctt gcggacaaac gcgtccggta atttccggca gggtgttagt
     8701 ctggtgagaa agctcgacgg cggcgtcgat gtttccggct ttcaccagtt caatccactg
     8761 tggaatatgg ttatgcagcg ggcaggtcca ttcacaaacg ctgtgctcgc cgcacttaag
     8821 gcagcgcgag gcttcccgtt gtgcctgatc ggcacggaat ggcagataaa tttcatcaaa
     8881 accggttttg cgcgcttcaa tcgccagttt gtccggctcg ccacgcgcgg gcgttgcctg
     8941 catttgctcg actttactca ttaccggcat ttcttgcgcc gcggtactgg catgccacgg
     9001 ttgatgttcc tgacgtgcgg tacgcaagcg gcgggatttc gccatgcctg agagtgcggc
     9061 gtcagtgacc agttgcagcg cgtctgccgg gcaattctcg acacaagcag gaccgttttc
     9121 gcgccctgca caaaggtcgc atttatgcgc cgtggctttt acttttcctg ccgcgacggg
     9181 ggtcaggacg atttgcatcg taccaaatgg acaggccacc acgcaggatt tacagccaat
     9241 acacttttgc tgattgacct gaatgctgtc atcaacgtgg ctgattgcgc cattagggca
     9301 gctacgggcg cagggcgcat cttcacaatg gtgacaggtc acggcactac gttgctgctg
     9361 atgtttgata accgtaattc ggggatgaaa atggtgttgg ctcaggacat gttgctcatc
     9421 attgtgagcc atgacacagg cgatttcaca agcatgacaa cccagacatt gctgactgtt
     9481 ggccataata aaacgattca taacgacctt cttttttggt tgtaaaaacc ttattcttta
     9541 tatgagtgtt gttattaccc gacttacagg ggaatcggca atgttcatgt gcccagaata
     9601 agtaactatt tcgctataaa ctgtggcaga tcaaataatc ccatcactga ctaaatttcg
     9661 tttcagcgaa ctggaacatt aatgattttt tgtggagaag acgcgtgtga ttgttaaacg
     9721 acccgtctcg gccagtctgg cccgggcctt tttttacatt gtgctgctgt cgattctttc
     9781 cacgggtatc gctctgctaa cgctggcgag cagtttacgc gacgctgagg ctatcaatat
     9841 tgccggatcg ctgcgtatgc agagttaccg cctgggctac gacttgcaaa gtggcagtcc
     9901 acaactcaat gcacatcgcc agttatttca gcaggcactg cattcaccgg tattaaccaa
     9961 tctcaacgtc tggtatgtgc cagaggcagt aaaaacccgc tatgcgcatc tgaatgccaa
    10021 ttggctggag atgaataatc ggctcagcaa gggcgacttg ccgtggtatc aggctaatat
    10081 taataattat gttaatcaga tagacctgtt cgtgctggct ttacagcact acgctgaacg
    10141 caaaatgctg ctggtggtgg caatttccct ggctggcggc atcggtattt tcacgctggt
    10201 cttttttact ttgcgccgca tacgccatca ggtggttgcc ccgctgaatc agctggttac
    10261 cgccagtcag cgtattgaac acgggcagtt cgactcgccg ccgctggata ccagcctgcc
    10321 gaatgagctt ggcctgcttg caaaaacctt taaccagatg tcgagcgagc tgcataaatt
    10381 gtaccgttcg ctggaggcgt cagtagaaga aaagacccgc gatctccacg aggccaagcg
    10441 tcgtctggag gtgttgtatc agtgttcgca ggcgctaaac accagccaga ttgatgtgca
    10501 ttgtttccgc catattttgc agattgttcg cgacaatgaa gcggctgaat atctggagtt
    10561 aaatgtcggt gacaactggc ggattagcga agggcagcca aacccggaat tgccgatgca
    10621 gattttaccg gtgacaatgc aagagacggt ttacggcgaa ctgcactggc aaaatagtca
    10681 cgtttcatca tcagaaccgc tgcttaacag cgtttcgtcg atgctgggac gcggtttgta
    10741 ctttaatcag gcgcagaagc attttcagca gttattgttg atggaagaac gtgcgaccat
    10801 cgcccgcgaa ttgcacgact cgctggctca ggtactttct tacttacgta tccagttgac
    10861 gttactgaag cgttcgatac cggaagataa cgccaccgca caaagtatca tggccgattt
    10921 ttcccaggcg ttgaatgatg cttatcggca gttacgcgag ctgttgacta ctttccgcct
    10981 gacgctgcag caggcggatc tcccctccgc gttgagggaa atgctggata cgttacaaaa
    11041 tcaaaccagc gccaaactga ccctcgactg ccgtctgcca accctggcgc tggatgcgca
    11101 aatgcaggtg catttgttgc aaattattcg cgaagcggtg ctgaatgcga tgaagcacgc
    11161 caacgccagc gaaatcgccg ttagctgcgt caccgcgccg gatggcaatc acacagtcta
    11221 tattcgcgac aacgggattg gcatcggtga accgaaagaa cccgaaggcc attatggtct
    11281 gaatatcatg cgcgaacgcg cggaaagatt gggtgggacg ctgacttttt cacaaccttc
    11341 cggcggcggc acgttagtga gtattagctt tcgctctgcg gagggtgagg aaagtcagct
    11401 gatgtaatgc ctcttattga ccaaagaata gtggcactta aggttcagta taaaagggca
    11461 tgataattta cattaactcc tttttttctc cacgattggc tcgtaccttg ccgctacagt
    11521 gaagcaagcc aagcctacaa cgatacgcag aaacacgagg tcctctttta atggcgaatt
    11581 tctttattga tcgccccatt tttgcctggg tgctggcaat cctgttgtgt ctgacaggta
    11641 ccctggcgat tttttcattg cccgttgaac aataccccga tctcgcgcca ccgaatgtgc
    11701 gagtgaccgc taactatccc ggcgcatcgg cccagacgct ggaaaacacc gtgacccagg
    11761 ttatcgagca aaatatgacg ggcctcgata atctgatgta tatgtcatct cagagcagcg
    11821 gcaccggtca ggcatctgtc acgttaagtt ttaaagcagg caccgatccg gacgaagccg
    11881 tgcagcaagt acaaaaccag ctgcaatcag ccatgcgaaa gttaccgcag gcggtgcaaa
    11941 atcagggcgt gacggtgcgt aaaaccggcg ataccaatat tctgaccatt gccttcgtct
    12001 ctaccgatgg ttcgatggat aaacaggata ttgccgatta cgttgccagt aatattcagg
    12061 atccgttaag ccgcgtaaat ggcgtcgggg atatcgatgc ctatgggtcg caatattcca
    12121 tgcgtatctg gctggacccg gcgaaactca acagtttcca gatgacggct aaagatgtca
    12181 ctgatgccat tgagtcacag aacgcgcaga ttgcggttgg gcaacttggt ggtacgcctt
    12241 ccgtcgataa gcaggcgctc aacgccacca ttaacgccca gtcactgctg caaacaccag
    12301 aacagttccg cgatatcacc ttgcgggtga atcaggacgg ctcagaggta aggctgggcg
    12361 atgtcgccac cgtcgaaatg ggggcggaga aatacgatta tcttagccgc ttcaatggta
    12421 agccagcctc cgggttgggg gtaaaactgg cctccggcgc gaacgaaatg gcgacagcgg
    12481 agctggtgct caatcgtctt gacgagctgg cgcagtattt cccgcatgga ctggaataca
    12541 aggtggcgta tgaaaccacc tcgtttgtta aagcctccat tgaagacgtg gtgaaaacgc
    12601 tgctggaagc tatcgctctg gttttcctcg ttatgtatct gttcctgcaa aacttccgcg
    12661 ccacgctgat acccactatc gccgtgccgg tggtgttgat gggaaccttc tccgtacttt
    12721 atgccttcgg ttacagcgtc aacaccttaa ccatgttcgc gatggtgctg gcgatcggtc
    12781 tgctggtgga tgatgccatc gtggtggtgg aaaacgtcga acgtattatg agtgaggaag
    12841 gactcactcc tcgcgaagcc acacgtaaat cgatggggca gatccagggg gcactggtcg
    12901 ggattgcgat ggtgctttcg gcggtatttg tgccaatggc cttcttcggc ggcaccaccg
    12961 gtgccatcta tcgccagttc tctattacca ttgttgcggc gatggtgctg tcagtactgg
    13021 tagcgatgat cctcactccg gctctgtgtg ccacgctact taagccactg aaaaaaggtg
    13081 agcatcacgg gcaaaaaggc ttttttgcct ggtttaacca gatgtttaac cgcaacgccg
    13141 aacgctacga aaaaggggtg gcgaaaattc tccaccgtag cctgcgctgg attgtgattt
    13201 atgtcctgct gcttggcggc atggtgttcc tgttcctgcg tttgccgacg tcgttcttac
    13261 cgctggaaga ccgtggcatg tttactacct cggtacagtt gcccagcggt tcaacccagc
    13321 aacagaccct gaaagtcgtt gagcaaatcg agaaatatta cttcacccat gaaaaagaca
    13381 acatcatgtc ggtgtttgcc accgtgggtt ctggccctgg gggtaacggg caaaacgtgg
    13441 cgcgaatgtt tatccgcctg aaagactgga gcgaacgcga cagtaagacc ggcacctcgt
    13501 ttgccattat cgagcgagca acgaaggcgt ttaacaagat taaagaagct cgcgttatcg
    13561 ccagcagccc gccagcaatt agcggtcttg gtagttctgc gggttttgat atggagttgc
    13621 aggaccacgc tggggcaggt cacgatgcgc tgatggcagc acgaaaccag ttgctggcgc
    13681 tggcggcgga aaatccggag ttaacccgtg tacgccataa cggcctcgac gacagtccgc
    13741 agttgcagat tgatatcgat cagcgtaaag ctcaggcgct gggcgttgct atcgacgata
    13801 ttaacgacac gctgcaaacc gcgtggggtt cgagctatgt gaatgacttt atggatcgcg
    13861 gtcgcgtgaa gaaagtctat gtgcaggcag ctgcgccgta tcgcatgctg ccagatgaca
    13921 tcaatctctg gtatgtccga aataaagatg gcggcatggt gcccttctct gctttcgcga
    13981 cctcacgctg ggagacaggc tcaccgcgtc tggaacgcta taatggttat tctgcggttg
    14041 agattgttgg ggaagccgca ccgggggtca gtaccggtat ggcgatggat attatggaat
    14101 cgttagtgaa gcagttgcca aacggctttg gtctggagtg gacggcgatg tcgtatcagg
    14161 agcggctttc cggcgcgcag gctccggcgc tgtacgccat ttccttgctg gtggtattcc
    14221 tgtgtctggc tgcgctgtat gaaagctggt cggtgccgtt ctcggtaatg ctggtcgtgc
    14281 cgctgggggt tatcggcgcg ctgctggcaa cctggatgcg cgggctggaa aacgatgttt
    14341 acttccaggt gggcctgtta acggtcattg gtttatcggc gaaaaacgcc atcctgatcg
    14401 tcgagtttgc taacgagatg aaccaaaaag gccacgacct gtttgaagcg acgctccacg
    14461 cctgccgtca gcgtttacgc ccgattctga tgacctcgct ggcatttatc ttcggcgtat
    14521 tgccaatggc aaccagcaca ggtgccggtt ccggtggtca gcatgcggtg ggtactggcg
    14581 taatgggcgg gatgatttcg gccactattc tggctattta cttcgtgccg ctgttctttg
    14641 tgctggtgcg ccgccgcttc ccgctgaagc cgcgcccgga ataagcaata aaaaaggcga
    14701 catgccaatg tgtcgccctt ttcaactttc cgattaagaa cctgctcagc ggattcttgc
    14761 tgtttgtact ttgtctcagg aattacttac gaagcataac ttcgataaag tctttccagt
    14821 tccccagttc acgttcaatc ataacaacct ctcttataat tatgggtatt ctacggaaac
    14881 aatataccgt ggtgaagcta atttactcga ttgctgtgat gactacctcc gggggacaaa
    14941 ccttatgtaa atactatggt cctgcggtga tgatttgtat gtgatgca
//
LOCUS       DAPHCV010000110        14942 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.110, whole genome shotgun sequence.
ACCESSION   DAPHCV010000110 DAPHCV010000000
VERSION     DAPHCV010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.110"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(658..742)
                     /locus_tag="RH033_003687"
     tRNA            complement(658..742)
                     /locus_tag="RH033_003687"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(706..708),aa:Leu,seq:caa)
     gene            938..1957
                     /gene="ahr"
                     /locus_tag="RH033_003688"
     CDS             938..1957
                     /gene="ahr"
                     /locus_tag="RH033_003688"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HDV2992812.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLPVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2087..3589
                     /locus_tag="RH033_003689"
     CDS             2087..3589
                     /locus_tag="RH033_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HDV2992813.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGVAEEGTSSEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGIGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGFQASTEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(3750..4832)
                     /gene="lptG"
                     /locus_tag="RH033_003690"
     CDS             complement(3750..4832)
                     /gene="lptG"
                     /locus_tag="RH033_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDV2992814.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(4832..5932)
                     /gene="lptF"
                     /locus_tag="RH033_003691"
     CDS             complement(4832..5932)
                     /gene="lptF"
                     /locus_tag="RH033_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDV2992815.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6199..7710
                     /gene="pepA"
                     /locus_tag="RH033_003692"
     CDS             6199..7710
                     /gene="pepA"
                     /locus_tag="RH033_003692"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDV2992816.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFEQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            7844..8287
                     /gene="holC"
                     /locus_tag="RH033_003693"
     CDS             7844..8287
                     /gene="holC"
                     /locus_tag="RH033_003693"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HDV2992817.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8287..11142
                     /gene="valS"
                     /locus_tag="RH033_003694"
     CDS             8287..11142
                     /gene="valS"
                     /locus_tag="RH033_003694"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDV2992818.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIKPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEDQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11196..12392)
                     /locus_tag="RH033_003695"
     CDS             complement(11196..12392)
                     /locus_tag="RH033_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HDV2992819.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLTVAGYIIFDQILNEYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            12585..13088
                     /locus_tag="RH033_003696"
     CDS             12585..13088
                     /locus_tag="RH033_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV2992820.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13134..13550)
                     /gene="rraB"
                     /locus_tag="RH033_003697"
     CDS             complement(13134..13550)
                     /gene="rraB"
                     /locus_tag="RH033_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HDV2992821.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            13712..14725
                     /gene="argF"
                     /locus_tag="RH033_003698"
     CDS             13712..14725
                     /gene="argF"
                     /locus_tag="RH033_003698"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDV2992822.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTKCRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3919 a         3713 c         3827 g         3483 t
ORIGIN      
        1 aaaactagcc acttaactaa taattataaa tttcatcttt tacaccttgc atttttataa
       61 ccaatgaaat ctacaacaag caactgatgg ataatccgat attggtcgca tttaatggca
      121 tggtagcaaa gttttcatca catacctcct ttgcaccaga ataaacgcca gagatctgaa
      181 attgcgagta cactacagaa attgtactgc cggtcccatc agcataaaac catagttaca
      241 caggtaatca tcattattcg ccaagataat aaatattatt cggtagaata gcgttctcag
      301 gtggtcagta aaattgtctt caacaagatg tctgcgcatc tcgtctgcta gccctacata
      361 actgctctcg accgaaacta tttattgaat gttcaccaag gtgggcgtgg agacaaaatg
      421 acgtcttttg atattagcta cctgagccac atggtcaaaa cgttcaagta gcataccgaa
      481 accaccctgc ccacgtaatg tggacgatgc agcttccagc cagagcaaac ttgggaccat
      541 cgactacaaa atgaagaata ctgaactgct tttctggcag gcctatatac tgcacggata
      601 tgggtgattt cagacacaaa aaaagccgct cttgagcgac tcgatttgca tacggtgtgg
      661 tgccgaaggc cggactcgaa ccggcacgta tttctacggt tgattttgaa tcaactgcgt
      721 ctaccgattt cgccacttcg gcactgaaga ggtatgcgga aaacgttgtg gattatacct
      781 gtcgcacgcc accatgcaag cgccagcttg ctcgcacctc gctaagtgct gaaattttca
      841 gcattgcata cagcgatgtg taacctttgt cacactccag gcaccccgcc ctgccatgct
      901 ctacacttcc cagacaacac cagagaagga ccaaaaaatg tcgatgataa aaagctatgc
      961 cgcaaaagaa gcgggcggcg aactggaagt ttatgagtac gatcccggtg agctgaagcc
     1021 acaagatgtt gaagtgcagg tggattactg cgggatttgt cattccgatc tgtcgatgat
     1081 cgataacgaa tggggatttt cacaatatcc actggttgcc gggcatgagg tgattggtcg
     1141 cgtggtggcg ctcgggagtg ccgcacagga taaaggtttg caggtcggtc agcgtgtcgg
     1201 gattggctgg acggcacgca gctgcgggca ttgcgatgcc tgtattagcg gtaatcagat
     1261 caactgcgag caaggtgcgg tgccgacgat tatgaatcgc ggtggctttg ccgagaagtt
     1321 gcgtgcggac tggcaatggg tgattccact gccagaaaat atcgacattg aatctgccgg
     1381 accgctgttg tgcggcggta tcacggtctt taaaccgctg ttgatgcacc atatcactgc
     1441 caccagccgc gttggggtaa ttggtattgg cgggctgggg catatcgcta taaaacttct
     1501 gcacgcaatg ggatgcgagg tgacagcctt tagttctaat ccagcgaaag agcaagaagt
     1561 gctggcgatg ggtgccgata aagtggtgaa tagccgcgat ccacaggcac tgaaagcact
     1621 ggcggggcag tttgatctca ttatcaacac cgtcaacgtc agcctcgact ggcagcctta
     1681 ttttgaggcg ctgacgtatg gcggtaattt ccatacggtc ggtgcagttc tcacgccctt
     1741 gccggttccg gcctttacgc tgattgcggg cgaccgcagc gtctctggct ctgctactgg
     1801 cacgccttat gagttgcgta agctgatgcg ctttgccgcc cgcagcaagg ttgcgccgac
     1861 caccgaactg ttcccgatgt cgaaaattaa cgacgccatc cagcatgtgc gggatggtaa
     1921 ggcgcgttat cgcgtggttc tgaaagccga cttctgatcc ctttgccggg tgcctgtcgc
     1981 ccggcactta aatctctttg gaaggcgtct cgcaatctgc tgaatttagc ggtgaagagt
     2041 gcgcagccgt tgcctatact ccaggcagga aactggagga aatctcatga gcgaacccct
     2101 gttaattgcc cgcacgccgg acacagaact ttttttactg ccgggaatgg ctaaccgtca
     2161 cgggctgatt actggcgcaa cggggacggg taaaaccgtt acgctgcaaa aactggcaga
     2221 gtctctatct gaaatcggcg tgccggtgtt tatggccgat gtgaaaggtg atttaacggg
     2281 agtagcggag gaaggtactt cttcggaaaa actgctcgca aggcttaaaa atatcggcgt
     2341 aaatgactgg caacctcatg ctaatccggt ggtggtgtgg gatatctttg gcgagaaagg
     2401 ccatccggtg cgggcgacgg tttcggatct tgggccgctg ttgctggcac gactgttgaa
     2461 tctcaacgat gtgcaatctg gcgtgctgaa tatcattttc cgcattgctg acgatcaggg
     2521 attgttgctg ctcgacttta aagatttgcg ggcgattacc cagtacatcg gcgataacgc
     2581 caaatccttc cagaatcagt acggaaatat cagtagcgca tcggttggtg ccatccagcg
     2641 cggattactg tcgctggaac agcaaggcgc agcacacttc tttggtgagc cgatgctgga
     2701 tatcaaagac tggatgcgca ccgatgccaa cggtaaaggc gttatcaata tcctcagcgc
     2761 cgagaaactt tatcagatgc cgaaactgta cgccgccagc ctgctgtgga tgctttcaga
     2821 gttatatgaa caattgcctg aagcgggcga tctggagaaa ccaaaactgg tgtttttctt
     2881 cgacgaagca catctgctgt ttaacgatgc accgcaggta ctgctggata agattgagca
     2941 ggtgataagg cttatccgct cgaaaggcgt gggcgtctgg ttcgtttcgc aaaacccgtc
     3001 agatattccg gataacgtgc tcgggcagct cggtaatcgc gttcaacacg ctttgcgggc
     3061 ttttacgccc aaagatcaga aagcagtaaa agctgcggcg caaaccatgc gggccaatcc
     3121 ggcgtttgat accgaaaagg caatccagga actggggatc ggcgaggcgt taatctcgtt
     3181 tctcgatgcg aaaggaagcc cttcagtggt ggaacgggcg atggtgattg ccccctgttc
     3241 acgaatgggg ccggtgacgg aagatgagcg taatggcttg attaatcact ccccggtgta
     3301 tggcaagtac gaggatgacg tggatcggga atccgcctat gagatgttgc aaaaaggctt
     3361 tcaggccagt accgagcagc aaaataatcc tcccgcgaaa gggaaagagg tggcggtgga
     3421 tgacggtatt cttggtggat tgaaggatat tttgtttggc actaccggac cacgcggcgg
     3481 gaagaaagat ggtgtggtgc aaacaatggc gaaaagcgcc gcccgccagg tgacgaatca
     3541 gattgtgcgt gggatgttgg ggagtttgct gggggggaga aaaaggtaag tcaggtgcga
     3601 tttgtctttg cctgatgcga cgctggcgcg ctttatcatg cctaaactac tgcaatatat
     3661 tgaattttca tgatttttca ggcatgaacg aagcacactt tgctaacaat aataaaggga
     3721 gctttcgctc cctttattcg ttcatcaggt tacgattttc tcattaacag ccacaggctg
     3781 attaagaaga agctggcgct tggcagcaac gcgccgataa tcggcgggat gccgtaaacc
     3841 aatgtcagcg ggccgaagat ctggtccagt acgtagaaga caaaaccgaa gctgataccg
     3901 gtgaccacac gcacgcccat cggtacgcta cgcagtgggc caaagataaa cgacagcgcc
     3961 atcagcatca tcaccgccac ggatagaggc tggaagattt tgctccacat gttgagttga
     4021 taacgtccgg catcctgacc gctcgacttt agatacttca catagttgtg caaaccactg
     4081 atggagagtg catccgggtc cagcgccacc acgcccagtt tgtctggcgt gaggttggtt
     4141 ttccaggtgc cgctcaccgt ctgcgaaccg gtgatctgtt tcggattggt cagatcagat
     4201 tcatcaacct gcgacagacg ccagacttta tgttccgggt caaacttcgc ggtagcggca
     4261 tagcgtacgg attgcagacg acgattctcg ttaaaggcat aaatactgat gccacctaac
     4321 tcttcgtcac ctttaacccg ctcaatgtag acgaagttgt tgccatcttt cgcccataag
     4381 ccttgctgag tggagagtaa cgagccgccg tacatcgcct gggcacggta gttacgcgcc
     4441 atctgctcgc cctgcggcgc gacccattca ccaatcgcca tcgtcagtaa gaccagcgga
     4501 atggcggttt tcatcaccga cagcgccacc tgcatacggg taaaaccaga agcctgcatc
     4561 accaccagtt cgctgcgctg cgccagcatc ccaagaccaa gcaacgcccc aagcagagcc
     4621 gccatcggga agaagatctg cacatctttc ggcacgctca gcaaggtata cattcctgcg
     4681 cctaacgcgt cgtaactccc ctgcccggct tttttcagct gatcgacaaa tttgataatg
     4741 cccgacagcg acaccagcat gaacagcgtc atcatgatgg tggtaaaaat agttttaccg
     4801 atatagcggt caagtacgcc aaaaggttgc atcacaccgc tcctttacgc gaaaaactgg
     4861 cacgcaggcg gcggaccggc acggtgtccc aaaggttgag aacaatcgcc aaagccagat
     4921 aaatcaggtt aacggtccac atccacagtg tcgggtccag cttaccttta ccgccgttcg
     4981 atttcaggga ggtctggatc aggaagaaaa gtagatacag cagcatggct ggcagcatcg
     5041 acagtacgcg tccctgacgt gggttaacca cgctcagcgg tacgaccata agtgccatca
     5101 taaacacggt gaataccaac gtgatacgcc agttcagttc tgcgcgagca cgatcggtgt
     5161 cagtgttcca caatgtgcgc atgtccatct ggtcggtatc gttcgggtcg agcgccaccg
     5221 cctggtgacc aatgatcgcc tgataatcct ggaagtccgt aatgcggaaa tcgcgtaaca
     5281 gtgcagtgcc ttcgaagcgc gttccctggt tgagagtgac gacctgggag ccgtcgcgca
     5341 gctgggttaa atgtccggaa tcggccacca ccacagaagg acgtgcatta ccttttggtc
     5401 gaatttgtgc gaggaacaca tctttgaaat cgctgccgtc aacgctttcg atgaacagca
     5461 ccgagctgcc attagtcgct tgctggaatt gcccttgcgc cagcgccgcc atgccagggt
     5521 tcgctttcgc ttctgctaac acttcatcct gatgacgcga tgaccacggt cccgcccaca
     5581 tcacgttaac cgccgcgacg attgccgtga ataccgcaag gatcattgcc gctttcacca
     5641 gaaccgcttt gctcaggccg caggcatgca ttaccgtaat ttcactttcg gtatacagtt
     5701 tgcccagcgt catcagcagc ccgaggaaca ggcttaatgg caggataagc tgcgccattt
     5761 ccggcacgcc caacccgaga agggagagca ccagattcgc cggaatatcg ccgtcaaccg
     5821 ctgcgccgag gatcctcact aacttttgac agaagaagat caaaagcaag atgaagagta
     5881 tcgccagctg gcttttgagc gtctcccgca ccagatatct tatgattatc actttaaata
     5941 cgcccgtaaa aactcgtctt ttgcaggatt ttagcttgtt tcatggctta aacgtcattt
     6001 attctcttga gtcgtcgaaa tcgtcgctaa gataattata ctcaacggat tcacctctca
     6061 gattttgttc tgacgtgcca atgccgtaat aacgttaaga ttaacacgaa gtcatcgcaa
     6121 cagcggacat gagttacgaa agcttgcaat tctatctgta gccaccgccg ttgtctttaa
     6181 gattcaggag cgtagtgcat ggagtttagt gtaaaaagcg gtagcccgga gaaacagcgg
     6241 agtgcctgca tcgtcgtggg cgtcttcgaa ccacgtcgcc tttctccgat tgcagaacag
     6301 ctcgataaaa tcagcgatgg gtacatcagc gccctgctac gtcggggcga actggaagga
     6361 aaaccggggc agacactgtt gctgcaccat gttccgaatg tactttccga gcgaattctc
     6421 cttattggtt gcggcaaaga acgtgagctg gatgagcgtc agtacaagca ggttattcag
     6481 aaaaccatta atacgctgaa tgatactggc tcgatggaag cggtctgctt tctgactgaa
     6541 ctgcacgtta aaggccgtaa caactactgg aaagtgcgtc aggctgtcga gacagcaaaa
     6601 gagacgctct acagtttcga gcagttgaaa acgaataaga gcgaaccgcg tcgcccgctg
     6661 cgtaagatgg tgttcaacgt gccgacccgc cgtgaactga ccagcggtga gcgcgcgatc
     6721 cagcacggtc tggcgattgc cgccgggata aaagcagcga aagatctcgg taatatgccg
     6781 ccgaatatct gtaacgccgc ttatctcgcc tctcaggcgc gccagctggc tgacagctac
     6841 agcaagaatg tcatcacccg cgttatcggc gaacagcaga tgaaagagct gggaatgcac
     6901 tcttatctgg cggtcggtca gggttcacaa aacgaatcgc tgatgtcggt gattgagtac
     6961 aaaggcaacg cgtcggaaga tgcacgccca atcgtgctgg tgggtaaagg tttaaccttc
     7021 gactccggcg gtatctccat caagccttca gaaggcatgg atgagatgaa gtacgatatg
     7081 tgcggcgcgg cggcggttta cggcgtgatg cgtatggtcg cggagctgca actgccgatt
     7141 aacgttatcg gcgtgctggc aggctgcgaa aacatgcctg gcgggcgagc ctatcgtccg
     7201 ggcgatgtgt taaccaccat gtccggtcaa accgttgaag tgctgaacac cgatgccgaa
     7261 ggccgcctgg tactgtgcga cgtgttaact tacgttgaac gttttgagcc ggaagcagtg
     7321 attgacgtgg cgacgctgac cggtgcctgc gtgatcgcgc tgggtcatca tattactggt
     7381 ctgatggcga accataatcc gctggcccat gaactgattg ccgcgtctga acaatccggt
     7441 gaccgcgcat ggcgcttacc gctgggtgac gagtatcagg aacaactgga gtccaatttt
     7501 gccgatatgg cgaacattgg cggtcgtcct ggtggggcga ttaccgcagg ttgcttcctg
     7561 tcacgcttta cccgtaagta taactgggct cacctggata ttgcaggaac cgcctggcgt
     7621 tctggtaaag caaaaggcgc aaccggtcgt ccggtagcgt tgctggcaca gttcctgtta
     7681 aaccgcgctg ggtttaacgg cgaagagtaa gtttgcgttt taccctcacc ctaaccctct
     7741 ccctaaggga gagggaaccg atcgagcgca atagaaattc attgctcatc tttcagggtg
     7801 aggaatgcag caatatttaa atccaccaca agaagcccca tatatgaaaa acgcaacgtt
     7861 ctaccttctg gacaatgaca ccaccgtcga tggcttaagc gccgttgagc agttggtgtg
     7921 tgaaattgcc gcagaacgtt ggcgcagtgg taagcgcgta ctcatcgcct gtgaagatga
     7981 aaagcaggct taccgactgg atgaagccct gtgggcacgt ccggcagaaa gctttgttcc
     8041 gcataattta gcgggagaag gaccgcgcgg cggtgcgccg gtggagatcg cctggccgca
     8101 aaaacgcagc agcagcccgc gcgatatatt gattagtctg cgaacaagct ttgcagattt
     8161 tgccaccgct ttcacagaag tggtagactt cgttccttat gaagattctc tgaaacaact
     8221 ggcgcgcgaa cgctataaag cctaccgcgt ggctggtttc aacctgaata cggcaacctg
     8281 gaaataatgg aaaagacata caacccacaa gatatcgaac agccgcttta cgagcactgg
     8341 gaaaagcagg gctactttaa gcctaatggc gatgaaagcc aggaaagttt ctgcatcatg
     8401 atcccgccgc cgaatgtcac cggcagtttg catatgggtc acgccttcca gcaaaccatc
     8461 atggatacca tgatccgcta tcagcgcatg cagggcaaaa acaccctgtg gcaggtcggt
     8521 actgaccacg ccgggatcgc tacccagatg gtcgttgagc gcaagattgc cgcagaagaa
     8581 ggtaaaaccc gtcacgacta cggccgcgaa gctttcattg acaaaatctg ggaatggaaa
     8641 gcggaatccg gcggcaccat tacccgtcag atgcgccgtc tcggcaactc cgtcgactgg
     8701 gagcgtgaac gcttcaccat ggacgaaggc ctgtccaatg cggtgaaaga agttttcgtt
     8761 cgtctgtata aagaagacct gatttaccgt ggcaaacgcc tggtaaactg ggacccgaaa
     8821 ctgcgtaccg ctatctctga cctggaagtg gaaaaccgcg aatcgaaagg ttcgatgtgg
     8881 cacatccgct atccgctggc tgacggcgcg aaaaccgcag acggtaaaga ttatctggtg
     8941 gttgcgacca ctcgcccgga aaccctgctg ggcgataccg gcgtggccgt taacccggaa
     9001 gatccgcgtt acaaagatct gattggcaaa tatgtcattc taccgctggt taaccgtcgt
     9061 attccgatcg ttggcgacga acacgccgac atggaaaaag gcaccggctg cgtgaagatc
     9121 accccagcgc acgactttaa cgactatgaa gtcggtaaac gtcacgccct gccgatgatc
     9181 aacatcctga cctttgacgg cgatatccgt gaaagcgccc aggtgttcga taccaaaggt
     9241 aacgaatctg acgtttattc cagcgaaatc ccggcagagt tccagaaact ggagcgtttt
     9301 gctgcacgta aagccgtcgt tgctgcggtt gacgcgctcg gcctgctgga agaaattaaa
     9361 ccgcacgacc tgaccgttcc ttatggcgac cgtggtggcg tggttatcga accgatgctg
     9421 accgaccagt ggtacgtacg tgccgatgta ctggcgaaac cggcagttga agcggttgag
     9481 aatggcgaca ttcagttcgt accgaagcag tacgaaaaca tgtatttctc ctggatgcgc
     9541 gatattcagg actggtgtat ctctcgtcag ctatggtggg gtcaccgtat cccggcatgg
     9601 tatgacgaag cgggtaatgt ttatgttggc cgcaacgaag acgaagtgcg caaggaaaat
     9661 aacctcggtg ccgacgttgc cctgcgtcag gacgaagacg ttctcgacac ctggttctct
     9721 tctgcgctgt ggaccttctc tacccttggc tggccggaaa ataccgacgc actgcgccag
     9781 ttccacccaa ccagcgtgat ggtatctggt ttcgacatca tcttcttctg gattgcccgc
     9841 atgatcatga tgactatgca cttcatcaaa gatgaaaatg gcaaaccgca ggtgccgttc
     9901 cacactgttt acatgaccgg tctgattcgt gacgacgaag gccagaagat gtccaaatcc
     9961 aagggtaacg ttatcgaccc gctggatatg gttgacggta tttcgctgcc agaactgctg
    10021 gaaaaacgta ccggcaatat gatgcagccg cagctggcgg acaaaatccg taagcgcacc
    10081 gagaaacagt tcccgaacgg cattaagccg cacggcaccg atgccctgcg cttcaccctg
    10141 gcggcgctgg cgtctaccgg tcgtgacatc aactgggata tgaagcgcct ggaaggttac
    10201 cgtaacttct gtaacaagct gtggaacgcc agccgctttg tgctgatgaa cacagaagat
    10261 caggattgcg gcttcaacgg cggcgaaatg acgctatcgc tggcggaccg ctggattctg
    10321 gcggagttca accagaccat caaagcgtac cgcgaagcgc tggacagctt ccgcttcgat
    10381 atcgccgcag gcattttgta tgagttcacc tggaaccagt tctgtgactg gtatctggag
    10441 ctgaccaagc cggtaatgaa cggtggaaca gaagccgaac tgcgcggtac tcgccatacg
    10501 ctggtgactg tactggaagg tctgctgcgc ctcgcgcatc cgatcattcc gttcatcacc
    10561 gaaaccatct ggcagcgtgt gaaagtactt tgcggtatca ctgccgacac catcatgctg
    10621 caaccgttcc cgcagtacga tgcatctcag gttgatgaag ccgcactggc tgataccgag
    10681 tggctgaagc aggcgatcgt tgcggtacgt aacatccgtg cagaaatgaa catcgcgccg
    10741 ggcaaaccgc tggagctgtt gcttcgtggt tgcagcgcgg atgcagagcg tcgcgtaaat
    10801 gaaaaccgtg gcttcctgca aacgctggcg cgtctggaaa gcatcaccgt gctgcctgcc
    10861 gatgacaaag gtccggtttc cgttaccaag atcatcgacg gcgcagagct gctgatcccg
    10921 atggctggcc tcatcaacaa agaagatgag ctggcgcgcc tggcgaaaga agtggcgaag
    10981 atcgaaggtg aaatcagccg catcgagaac aagctggcaa atgaaggctt tgtcgcccgc
    11041 gcaccggaag cggttatcgc gaaagagcgt gagaagctgg aaggttatgc agaagcgaaa
    11101 gccaagctga ttgaacagca ggctgttatc gccgcactgt aatcaacaca gtgatgaaaa
    11161 cgaaggccgg agcatgctcc ggcctttttt atctcttaca gaaatggtaa agaaggcata
    11221 actccccgag aaatactggc taagaagcct ttatctggtt gttcttcttt attgattaaa
    11281 ggaaggtcat ccaaatcgcc cagcaaatac gtattttttg cctgccagtc aatcatccat
    11341 attttcagca gtggataagc cagaccgccc gtaatcccgg atatcaccac caacgcacac
    11401 atgcgataaa gcataccgtg gtacgttaaa gttgagcgaa aacgaatacg cccatcattc
    11461 agtgacaggt tgctcataaa atggtttcgc aacgacaccg ttaaataact ggtgctaaca
    11521 gcaatcccaa aataatagat taactgcgcg attatcattt tacgctgcat ttccataaat
    11581 tgctgtaaat tctcaatatc atcatttgca tacacagaac tatcatactc atttaatatt
    11641 tgatcgaaga taatataacc agcaacagtg agaaaaggca ataaagctaa aaatgctaga
    11701 atggcatatt ttatacaata cgtagtatct aatttcacct tgaaacgatg tataccaaaa
    11761 ctggtattgc tccagagaaa actcattacc agactatata aagtaccatt aaaaatacca
    11821 atggaaacaa taccaactat tgccatcaga accacagata taataacact acttgaacta
    11881 ttggcatgta gcatctttgt cgagataaag aaaacagtcc ccatcccaat ggccattaaa
    11941 atcggcaaga aaaaggttac ccaccagaac cctttcaaag agcaattaaa actaaatctt
    12001 acgccgttga gactggtcat taaggcctga taacgcagtc ctttggctgc cataaatata
    12061 agcaaaacca acagtaacaa agtcaaaaca cagccaacaa gcggcatatc tgctgacact
    12121 gtcattaaga ttgcgaaata gaaaaaaaca aaaacaagac aactaacaaa aacattccca
    12181 ccggtaattc cataagaaaa tcgttgtccg ttaacttcca tattagcata aagataacgc
    12241 ttacatttca ttaatgccca tggtaaatag atacctagcg ttataatcgt taacaacaca
    12301 ttcaccacac aaataagaaa atatctctca cctgtaccat gaaaaacaaa agaatgggac
    12361 ggaacatcca tttcattaat aacttgagcc ataaaaattc cctgaattaa aaaatccatt
    12421 gatataattt atagatacag acagtaaaat gttctgccac gttacaatac tcttatcagt
    12481 aataggctgg caaaaacttt attgctttat aagacgcttg cgcacgtaag aagagagtgg
    12541 tattaaacaa atgtataatt actttatttt tattgggtta aactatgaat aacattgcgc
    12601 cgcaatcacc tgtaatgcgt cgcctgacgc tgcaggataa tcctgctatc gcccgcgtca
    12661 ttcgtcaggt atccgccgaa tacggtctta ccgctgataa aggctacacc gtcgccgatc
    12721 cgaatcttga cgagctgtat caagtgtata gtcagcctgg ccatgcatat tgggtggttg
    12781 agtacgaggg tgaagtggtc ggcggcggtg ggatagcgcc attaacaggt agtgaagcgg
    12841 acatttgcga actgcagaaa atgtactttc ttcccgccat tcgcggtaaa gggctggcaa
    12901 aaaaactggc cttaatggcg atggagcagg cgcgagaaat gggtttcaaa cgctgctatc
    12961 tggaaacgac cgcttttttg aaagaagcga ttggtttata tgaacattta ggttttcaac
    13021 atatcgacta tgcacttggc tgcacgggcc atgttgattg tgaagtgcgg atgttgcgcg
    13081 aactctgaaa aaatgcccgc tagtatgtgc tatcgggcat tttaatcaat taattagtgg
    13141 cgaaccccgt catcgtcttc atcgacaaaa tcttcatcgt cgccatcttc gccgttagga
    13201 tcttcaaagt aagtgcccca accgtcgtac tcaacgtcaa atttctccgc cagcgtcatc
    13261 agttgttcaa cctgggcatc gatcagatcg gcattcaacg cgcactcgct gaggatgtcg
    13321 cagcaaatca cgatatcacc atcttcaact tccagctctt ctggatcggt cacttcgtaa
    13381 ccgagtttaa acgcttcaac tgccgctttt tccagagttt ccagatcgtc tgcggaaaga
    13441 tggtgttcga tggtgtacag cgcgtccgga tcgctgccat cttccagtaa ttcttcaata
    13501 atcaaacgtg tttcttcacg ctgttcttcc agttgttccg ggtttgccat ggctcattcc
    13561 tcttaatgtc ctgccgatac tcttattgtc acacaccact gacattgcct ccacctttgt
    13621 gataaagatt tatgctttag acttgcaaat gaataatcat ccatataaat tgaattttaa
    13681 ttcattgagg cgttagccac aggagggatc tatgtccggg ttttatcata agcatttcct
    13741 gaaattactc gatttcacgc cagctgaact taacagcctg ctgcagttag ccgcgaagct
    13801 gaaagctgat aagaaaagcg gtaaagaaga agccaaactc actggtaaaa acatcgcgct
    13861 catcttcgaa aaagactcga cccgtacccg atgctctttc gaagttgccg catatgacca
    13921 gggtgctcgc gttacttatc tcggcccaag cggtagtcag attggtcata aagagtcgat
    13981 taaagacact gcccgcgtgc ttggtcgcat gtatgacggt attcagtatc gcggctatgg
    14041 tcaggagatt gtcgaaacac tggcggaata cgctggcgtg ccggtatgga acggcctgac
    14101 caatgagttt caccccacgc agttgctggc ggatctcctc accatgcagg agcatttacc
    14161 cggtaaagca ttcaacgaaa tgacgctggt ctatgcaggt gacgcacgta acaacatggg
    14221 caattcgatg ctcgaagctg cggcgcttac cggtctggat ttgcgtctgg tcgcgccaca
    14281 ggcatgctgg ccagaagctg cgctggttac gaaatgccgc gccctggcac aacaaaatgg
    14341 cgggaatatt acgctgacgg aagatgtagc taaaggagtt gaaggtgctg actttatcta
    14401 taccgatgta tgggtgtcga tgggtgaagc aaaagaaaaa tgggctgaac gcattgcatt
    14461 actgcgtgat tatcaggtga acagcaagat gatgcagttg accggtaacc cggaggtcaa
    14521 attcctccac tgcctgcccg cgtttcatga cgaccagacg acgcttggta agaaaatggc
    14581 ggaagagttt ggcctacatg gcggaatgga agtgactgat gaggtcttcg aatctgccgc
    14641 cagcattgtt tttgatcagg cggaaaaccg catgcatacc atcaaagcgg tgatggtcgc
    14701 gacgctcagt aaattaaata attaatatct ctataaagat gccgggaaat acaaaaacat
    14761 cccggcatca cattatttta tgcagtgata gttttttctt ccagccaaat ttttctattg
    14821 ctatacgtaa attttccgga ggatttagtg tatttaaacg ctgttggtca atatatggcg
    14881 atacattaac cttcgattta aagatagttg catcattcta aatctaagtt tgaattcatt
    14941 at
//
LOCUS       DAPHCV010000111        14299 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.111, whole genome shotgun sequence.
ACCESSION   DAPHCV010000111 DAPHCV010000000
VERSION     DAPHCV010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.111"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..706
                     /gene="gspC"
                     /locus_tag="RH033_003699"
     CDS             <1..706
                     /gene="gspC"
                     /locus_tag="RH033_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HDV2992823.1"
                     /translation="FHTTIVKNGQIINQPTNAFQSEFSLAALWRNENHAGVKDANPVA
                     VNQETPKLSIALNGIVLTSNDETSFVLINEGSEQNRYSLNEALESAPGTFIRKINKTS
                     VVFETHGHYEKVTLHPGLPDIIKQPDSESQNVLADYIIATPIRDGEQIYGLRLNPRKG
                     LNAFTTSLLQPGDIALRINNLSLTHPDEVSQALSLLLTQQSAQFTIRRNGVPRLINVS
                     VAELTGMNGQRHERTQ"
     gene            690..2642
                     /gene="gspD"
                     /locus_tag="RH033_003700"
     CDS             690..2642
                     /gene="gspD"
                     /locus_tag="RH033_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HDV2992824.1"
                     /translation="MKGLNKITCCLLAALLMPCAGHAENEQYGANFNNADIRQFVEIV
                     GQHLGKTILIDPSVQGTISVRSNDTFSQQEYYQFFLSILDLYGYSVITLDNGFLKVVR
                     SANVKTSPGMIADSSRPGVGDELVTRIVPLENVPARDLAPLLRQMMDAGSVGNVVHYE
                     PSNVLILTGRASTINKLIEVIKRVDVIGTEKQQIIHLEYASAEDLAEILNQLISESHG
                     KSQMPALLSAKIVADKRTNSLIISGPEKARQRITSLLKSLDVEESEEGNTRVYYLKYA
                     KATNLVEVLTGVSEKLKDEKGNERKPSSSGAMDNVAITADEQTNSLVITADQSVQEKL
                     ATVIARLDIRRAQVLVEAIIVEVQDGNGLNLGVQWANKNVGAQQFTNTGLPIFNAAQG
                     VADYKKNGGITSANPAWDMFSAYNGMAAGFFNGDWGVLLTALASNNKNDILATPSIVT
                     LDNKLASFNVGQDVPVLSGSQTTSGDNVFNTVERKTVGTKLKVTPQVNEGDAVLLEIE
                     QEVSSVDSSSNSTLGPTFNTRTIQNAVLVKTGETVVLGGLLDDFSKEQVSKVPLLGDI
                     PLVGQLFRYTSTERAKRNLMVFIRPTIIRDDDVYRSLSKEKYTRYRQEQQQRIDGKSK
                     ALVGSEDLPVLDENTFNSHTPAPSAR"
     gene            2652..4133
                     /gene="gspE"
                     /locus_tag="RH033_003701"
     CDS             2652..4133
                     /gene="gspE"
                     /locus_tag="RH033_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HDV2992825.1"
                     /translation="MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQV
                     GQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDIDQSVDLLSLSEEMPANEDLLNE
                     DSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
                     ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQ
                     LSLNNLGMTAADKQDLENLIQLPHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTV
                     EDPVEYELEGIGQTQVNTRVDMSFTRGLRAILRQDPDVVMVGEIRDTETAQIAVQASL
                     TGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVS
                     PQQAQMFKYHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQ
                     ALERLVRQQHNALIKNGLQKVISGDTSWDEVMRVASATLENEA"
     gene            4130..5326
                     /gene="gspF"
                     /locus_tag="RH033_003702"
     CDS             4130..5326
                     /gene="gspF"
                     /locus_tag="RH033_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HDV2992826.1"
                     /translation="MNYRYRAMTQDGQKLQGIIDANDERQARLRLREEGLFLLDIRPQ
                     KSSGVKTRRPRISHSELTLFTRQLATLSAAALPLEESLAVIGQQSSNKRLADVLNQVR
                     SAILEGHPLSDALQHFPTLFDSLYRTLVKAGEKSGLLAPVLEKLADYNENRQKIRSKL
                     IQSLIYPCMLTAVAIVVVIILLTAVVPKITEQFVHMKQQLPLSTRILLGLSDTLQHTG
                     PTLLATVFIVAVGFWLWLKRGNNRHRFHAMLLRVALIGPLICAINSARYLRTLSILQS
                     SGVPLLDGMNLSTESLSNLEIRKRLANAAENVRQGNSIHLSLEQTAIFPPMMLYMVAS
                     GEKSGQLGTLMVRAADNQETLQQNRIALTLSIFEPALIITMALIVLFIVVSVLQPLLQ
                     LNSMIN"
     gene            5336..5773
                     /gene="gspG"
                     /locus_tag="RH033_003703"
     CDS             5336..5773
                     /gene="gspG"
                     /locus_tag="RH033_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HDV2992827.1"
                     /translation="MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQK
                     AVSDIVALENALDMYKLDNHRYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADP
                     WGNDYVLVNPGEHGAYDLLSAGPDGEMGTEDDITNWGLSKKKK"
     gene            5781..6290
                     /gene="gspH"
                     /locus_tag="RH033_003704"
     CDS             5781..6290
                     /gene="gspH"
                     /locus_tag="RH033_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HDV2992828.1"
                     /translation="MNQQRGFTLLEMMLVLALVAITASVVLFTYGREDAANTRARETA
                     ARFTAALELAIDRATLSGQPVGIHFSDSAWRIMVPGKTPSAWRWVPLQEDAADESKND
                     WDEELSIHLQPFKPDDSNQPQVVILADGQITPFSLLMANAGTGEPLLTLVCSGSWPLD
                     QTLARDTRP"
     gene            6287..6664
                     /gene="gspI"
                     /locus_tag="RH033_003705"
     CDS             6287..6664
                     /gene="gspI"
                     /locus_tag="RH033_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HDV2992829.1"
                     /translation="MNKQSGMTLLEVLLAMSIFATVALALMSSMQGQRNAIERMRNET
                     LALWIADNQLKSQDTFNDENTSSSGKEIINGEEWNWRSDVHSSKDGTLLERTITVTLP
                     NGQKTALTRYQSIHDRSGQAQDD"
     gene            6657..7244
                     /gene="gspJ"
                     /locus_tag="RH033_003706"
     CDS             6657..7244
                     /gene="gspJ"
                     /locus_tag="RH033_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HDV2992830.1"
                     /translation="MINRQQGFTLLEVMAALAIFSMLGVLAFMVFSQASTLHQRSQKE
                     IQQFNQLQRTITILDNDLLQLVARRNRSTDKIMVLGEEAIFTTQSRDPQAPLNEAQTL
                     QTVHWYLRNHTLYRAVRTSVDGRKDQPAQAMLEHVESFLLESNSGESKEHPLSVTLHL
                     KTQQYGALQRRFALPEQLAREESPAQTQAGNNNHE"
     gene            7237..8220
                     /gene="gspK"
                     /locus_tag="RH033_003707"
     CDS             7237..8220
                     /gene="gspK"
                     /locus_tag="RH033_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HDV2992831.1"
                     /translation="MNNEQRGVALLIVLMLLALMAALAADMTSSFHGQLQRTRQVNHH
                     LQRQYDIELAEKLALASLTQDVKDNDRQTTLQQYWAQPQQLQLEDGNTVKWQLRDAQH
                     CFNLNALAKISDDPLASPDFPAQVFSALLINAGIDRGNTDEIVQSIADYIDVDDSPRF
                     HGAEDNFYQSQTPPRHSANQMLFLTGELRQIKGITENIYQRLIPYVCVLPTTELSINL
                     NMLTENDIPLFRALFLNNITDADARVLLQKRPREGWLTTDAFLYWAQQDFSGVKPLVA
                     QVKGHLFPYSRYFTLSTESISDEQSQGWQSHIFFNRKQQSAQIYRRTLQLY"
     gene            8235..9398
                     /gene="gspL"
                     /locus_tag="RH033_003708"
     CDS             8235..9398
                     /gene="gspL"
                     /locus_tag="RH033_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HDV2992832.1"
                     /translation="MPESLMVIRSSSTLRKHWEWMTFSADSVSSVHTPTDDLPLESLA
                     DQPGAGNVHLLIPPEGLLYRSLTLPNAKYKLTAQTLQWLAEETLPDNTQDWHWTVVDK
                     QNESVEVIGIQSEKLSRYLERLHTAGLNVTRVLPDGCYLPWEVDSWTLVNQQTSWLIR
                     SAAHAFNELDEHWLQHLAAQFPPENMLCYGVAPHGVAATNPLIQHPEIPSLSLYSADI
                     AFQRYDMLHGVFRKQKTVSKSGKWLARLAVSCLVLAILSFVGSRGIALWQTLKIEDQL
                     QHQQQETWQRYFPQIKRTHNFRFYFKQQLAQQYPEAVPLLYHLQTLLLEHPELQLMEA
                     NYSQKQKSLTLKMSAKSEANIDRFCELTQSWLPMQKTEKDPVSGVWTVRSSGK"
     gene            9395..9856
                     /gene="gspM"
                     /locus_tag="RH033_003709"
     CDS             9395..9856
                     /gene="gspM"
                     /locus_tag="RH033_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HDV2992833.1"
                     /translation="MIKSWWAEKSTSEKQLVAALAVLSLGVFCWLGVIKPIDTYIAEH
                     QSHAQKIKKDIKWMQDQASTHGLLGHPALTQPIKNILLEEAKRENLAITLENGPDNTL
                     TIHPVTAPLENVSRWLTTAQITYGIVIEDLQFTLAGNEQITLKHLSFREQP"
     gene            9856..10533
                     /gene="gspO"
                     /locus_tag="RH033_003710"
     CDS             9856..10533
                     /gene="gspO"
                     /locus_tag="RH033_003710"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase GspO"
                     /protein_id="HDV2992834.1"
                     /translation="MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLVHF
                     RQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGALFLYFCFALTLSVIDFRTQLLPD
                     KLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
                     LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLG
                     YLAWISY"
     gene            complement(10562..11038)
                     /gene="bfr"
                     /locus_tag="RH033_003711"
     CDS             complement(10562..11038)
                     /gene="bfr"
                     /locus_tag="RH033_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HDV2992835.1"
                     /translation="MKGDTKVINYLNKLLGNELVAINQYFLHARMFKNWGLKRLNDVE
                     YHESIDEMKHADRYIERILFLEGLPNLQDLGKLNIGEDVEEMLRSDLALELDGAKNLR
                     EAIGYADSVHDYVSRDMMIEILRDEEGHIDWLETELDLIQKMGLQNYLQAQIREES"
     gene            complement(11110..11304)
                     /gene="bfd"
                     /locus_tag="RH033_003712"
     CDS             complement(11110..11304)
                     /gene="bfd"
                     /locus_tag="RH033_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HDV2992836.1"
                     /translation="MYVCLCNGISDKKIRQAVRQFSPHSFQQLKKFIPVGNQCGKCVR
                     AAREVMEDELMQLPEFKESA"
     gene            complement(11473..14175)
                     /gene="chiA"
                     /locus_tag="RH033_003713"
     CDS             complement(11473..14175)
                     /gene="chiA"
                     /locus_tag="RH033_003713"
                     /EC_number="3.2.1.14"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chitinase/lysozyme"
                     /protein_id="HDV2992837.1"
                     /translation="MKLNIFTKSMIGMGLVCSALPALAMEVWNNQQGGNKYQVIFDGK
                     IYENAWWVSSTNCPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNC
                     PGDAKGNDASNPWRYVRAATATEISETSNPQSCTSAPQPAPDVKPAPDVKPAPDVQPA
                     PADKSNDNYAVVAWKGQEGSSTWYVIYNGGIYKNAWWVGAANCPGDAKENDASNPWRY
                     VRAATATEISQYGNPGSCSVKPDNNSGAVTPVDPTPETPVTPVTPTPDNNEPSTPADS
                     SNDYSLQAWSGQEGSEIYHVIFNGNVYQNAWWVGSEDCPRGTSAENSNNPWRLVRTAT
                     AAEMSQYGNPTTCEIDNGGVIVADGFQASKAYSANSIVDYNDAHYKTSVDQDAWGFVP
                     GGDNPWKKYEPAKAWSASTVYVKGDRVVVDGQAYEALFWTQSDNPALVANQNATGSNS
                     RPWKPLGKAQSYSNEELNNAPQFNPETLYASDTLIRFNGANYISQSKVQKVSPSDSNP
                     WRVFVDWTGTKERVGTPKKAWPKHVYAPYVDFTLNTIPDLAALAKNHNVNHFTLAFVV
                     SKDANTCLPTWGTAYGMQNYAQYSKIKALREAGGDVMLSIGGANNAPLAASCKNVDDL
                     MQHYYDIVDNLNLRVLDFDIEGTWVADQASIERRNLAVKKVQDKWKSEGKDIAIWYTL
                     PILPTGLTPEGMNVLSDAKAKGVELAGVNVMTMDYGNAICQSANTEGQNIHGKCATSA
                     IANLHSQLKGLHPNKSDAEIDAMMGTTPMVGVNDVQGEVFYLSDARLVMQDAQKRNLG
                     MVGIWSIARDLPGGTNLSPEFHGLTKEQAPKYAFSEIFAPFTKQ"
BASE COUNT         3704 a         3680 c         3399 g         3516 t
ORIGIN      
        1 tttccataca actatagtta aaaatggaca gatcattaat caaccaacaa acgcatttca
       61 gtctgagttc agccttgccg ctttatggcg taacgaaaat catgctgggg taaaagatgc
      121 aaatcccgtg gcagttaatc aggaaacacc taagctctct atagcgctta atggcattgt
      181 actcacatcg aatgatgaga cttcttttgt cttaattaat gaggggagcg aacaaaaccg
      241 atactccctc aacgaagccc ttgagtcagc gccgggaacg tttattagaa aaataaataa
      301 aaccagcgtt gtgtttgaaa ctcacgggca ttacgaaaaa gtaactctcc atccagggct
      361 acctgacatc atcaagcagc ctgattcaga aagtcaaaat gtactcgctg attatattat
      421 cgccacgccg atacgtgatg gagaacagat atacgggctt cgattgaacc ctcgtaaggg
      481 cctcaatgca tttactacca gcttgctaca accaggggat atcgccctca ggatcaataa
      541 tctttcgctg acccaccctg atgaggtttc acaggcatta agtttactgc taactcaaca
      601 aagtgctcag tttacaattc gtcgcaacgg cgtaccccgc ttgataaatg tttccgtcgc
      661 ggaacttaca ggaatgaatg gacagcgtca tgaaaggact caataaaatc acctgctgct
      721 tgctggcagc actactcatg ccttgtgcag ggcacgctga gaacgaacaa tacggcgcta
      781 acttcaataa cgccgatatc cgccagttcg tggaaatagt gggtcagcat cttggcaaaa
      841 cgatcctgat cgacccttcg gtacagggaa ccatttccgt acgcagtaat gatacgttta
      901 gccaacagga gtactaccag ttctttttaa gtattcttga tctttacggt tattccgtga
      961 tcacgctgga caatggtttt ctgaaagtgg tgcgctcagc taatgtaaaa acatcgccag
     1021 ggatgattgc tgacagttct cgtccaggcg taggtgatga gttggtcacc cgaatcgtac
     1081 cgcttgagaa cgttcctgct cgtgacctgg ctcccctgct ccgccagatg atggatgcgg
     1141 gtagcgtcgg taatgttgtg cattatgaac cctccaacgt tcttattctg accggtcgtg
     1201 cctccaccat taataaactg attgaagtca taaagcgcgt tgatgtcatc ggcacagaga
     1261 agcagcaaat tattcatctg gaatatgcgt cagcggaaga tctcgccgaa attttgaatc
     1321 aattaatcag cgaaagccac ggtaaaagcc agatgcctgc cctcctttcc gcgaagattg
     1381 tggcggataa gcgaaccaac tctcttatca tcagcgggcc ggaaaaagca cgccagcgca
     1441 tcacttcatt actgaaaagc cttgatgtag aagagagcga ggaaggaaat actcgggttt
     1501 attacctgaa atatgctaaa gccacgaatc tggtggaagt gctaaccggt gtttccgaaa
     1561 agctgaaaga tgaaaaaggg aatgagcgta agccctcctc ttctggcgcg atggataacg
     1621 tcgccattac cgccgatgaa cagactaact ctctggtcat taccgctgac cagtccgtcc
     1681 aggaaaaact cgccacggta attgcgcgtc tggacattcg ccgtgcacag gtgctggttg
     1741 aggcaatcat cgttgaagtt caggatggaa atggacttaa cctcggcgtg caatgggcga
     1801 ataaaaacgt tggagcacag caatttacca ataccggatt accgattttt aacgctgcgc
     1861 aaggtgtggc tgattataaa aagaatggtg ggatcaccag cgcgaatcct gcctgggata
     1921 tgtttagcgc ctacaatggc atggccgcag gctttttcaa tggcgactgg ggcgtattgt
     1981 tgactgcgct ggccagtaac aataaaaatg acatcctcgc caccccgagc atcgtaacgc
     2041 tggataataa actcgcgtcc ttcaacgttg gtcaggatgt gccggtgcta tctggctctc
     2101 agaccacttc tggggataac gtctttaata ctgtcgaacg caaaacggtg ggcacaaaac
     2161 tcaaagtcac tccgcaggtc aatgaaggcg acgcggtact gctcgaaata gagcaggagg
     2221 tttccagcgt tgactcttcg tctaattcga cgctcggccc gacatttaac acccgaacta
     2281 ttcaaaatgc cgtgctggtc aaaaccggtg aaacggtggt cctgggcgga ttgctggatg
     2341 atttttctaa agagcaagtg tcaaaggttc ctctgcttgg cgatattcct ttagtggggc
     2401 aactcttccg ctatacttcg accgagcgcg ctaaacgcaa cctgatggta tttatccgcc
     2461 caacgattat ccgtgacgat gatgtttatc gctcactgtc aaaagagaaa tacacccgct
     2521 accgtcagga gcaacaacag cgaatcgacg ggaaatcaaa agcgctggtt ggctcggaag
     2581 atttgccggt gctggatgag aacacgttca acagccatac ccctgcgccc tcggcacggt
     2641 aaggcattct tatgagaatt cactcaccgt accccgccag ttgggcgctg gcacaacgaa
     2701 ttggttatct ctattcagag ggcgagatta tttatctcgc cgatacgcca ttcgaacggt
     2761 tactcgatat tcaacgtcag gttggccagt gccagaccat gaccagcttg tcacaggctg
     2821 attttgaagc ccggctggaa gcggtgttcc atcagaatac cggtgagtcg caacagattg
     2881 cgcaggatat cgatcaatcc gtcgatcttc tctcgctttc ggaagagatg cccgcaaatg
     2941 aagatctcct gaatgaagat tcagcggcac cggttatccg cttgatcaat gcgattttga
     3001 gtgaggccat caaagaaacc gcctccgata tccacattga aacctatgaa aaaacaatgt
     3061 cgatccgttt tcgcatcgac ggcgttttgc ggacaatttt acagccaaac aaaaaactgg
     3121 cggcactgct tatctcccga attaaggtca tggctcgtct tgatatcgcc gaaaaacgta
     3181 ttccacagga tggacgtatt agtttgcgta tcgggcgacg taacatagat gtccgcgtat
     3241 ccacactgcc gtccatctat ggtgaacgcg ccgtgctccg tctgctggat aaaaacagcc
     3301 tccagctttc attgaacaac ctggggatga cggcagcgga taaacaggat ttagaaaatc
     3361 tcatccagct tccacacggt attatcctgg tgaccggacc gacaggctcc ggtaaaagca
     3421 ccacgctcta cgccatcctt tcggcgctga atactcccgg ccgcaatatt ctgacggtag
     3481 aagatcccgt ggaatatgag ctggaaggca ttgggcaaac gcaggtgaat acccgtgtgg
     3541 atatgtcttt cactcgcggc ctgcgcgcca tacttcgcca ggacccggat gtcgtcatgg
     3601 tgggggaaat tcgtgataca gaaaccgcgc agattgcggt tcaggcctcg ctcaccggcc
     3661 atctggtact ctcaacactc cacactaaca gtgcatcagg cgcagtgacc cggctccgcg
     3721 acatgggcgt cgaatcattc ctgctttcgt cttccctggc agggattatc gcgcaacgtc
     3781 tggttcgtcg cctgtgtccg caatgccgac aattcacgcc cgtatcaccc caacaagcgc
     3841 agatgtttaa atatcatcag ctcgcggtga caacaattgg cactcccgta ggctgccctc
     3901 attgccatca atccggctat caggggcgca tggcgatcca cgaaatgatg gtggtgacgc
     3961 cggaattacg ggccgctatt catgaaaatg tggatgaaca agcactggag cgactggtcc
     4021 ggcaacaaca caatgcctta atcaaaaatg gcctgcaaaa agtgataagc ggtgacacct
     4081 cctgggatga ggttatgcgc gtcgccagtg ccacgctgga gaacgaagca tgaattatcg
     4141 ctatcgcgcc atgacccagg atggtcaaaa attgcagggg atcattgatg ctaacgatga
     4201 acgtcaggca cgactgcggc tgcgtgaaga agggcttttc ctgctggata ttcgccccca
     4261 aaaaagttcg ggagtaaaaa cacgtcgccc gaggatcagc catagtgaac tgacgctttt
     4321 cacccggcag ttggcaacct taagcgcagc ggcattacct ctggaagaga gccttgccgt
     4381 aatcggtcaa caaagcagta ataaacgact ggctgatgtg ttaaatcagg tacgcagcgc
     4441 catccttgaa gggcatcccc tttccgatgc gttacaacat tttcccacgc ttttcgattc
     4501 gctctaccgt accctggtta aagcgggcga aaagagcggg ctgctggccc cggtgttgga
     4561 aaaactggct gattacaatg aaaatcggca gaaaatccgc agcaaactca ttcagtcgct
     4621 gatctacccc tgcatgctga ctgcggtggc gattgtggtc gtgattattc tcctcactgc
     4681 tgtcgtgccc aaaattaccg aacagttcgt gcatatgaag cagcaactgc cgctgagtac
     4741 acgcattctt ttaggtctga gcgacacgtt gcaacatacc ggcccgacat tactagcgac
     4801 agtgtttatt gtcgctgtag gtttctggct ctggttaaaa cgcggcaata accgccaccg
     4861 ttttcatgcc atgttgctgc gcgttgcact catcggcccg ctgatttgcg ccattaacag
     4921 cgcacgctat ctccgcactt taagtatttt gcaatccagc ggcgtccctc tgctggatgg
     4981 gatgaatttg tccaccgaaa gtctcagcaa cctcgaaatc cgcaagcgtc tggcaaatgc
     5041 ggcagaaaac gttcggcagg gtaacagcat tcatctttcg ctggaacaaa cggcaatctt
     5101 cccgccgatg atgctctaca tggtggcctc tggcgaaaaa agcgggcagc tcggcacgtt
     5161 aatggtcaga gcagcagata accaggagac actccaacaa aatcggatcg ccttaacgct
     5221 ctccatcttc gagccagcac tcattattac gatggcactg atcgtcctgt ttattgtcgt
     5281 ctcggtactc caacctcttc ttcaacttaa ctcaatgatt aattaaggaa agtatatgcg
     5341 cgcaacggat aagcaacgcg gttttacatt actggaaatt atggtggtga tcgtcatcat
     5401 tggcgtactt gccagcctgg tggttcccaa cctgatgggc aataaagaaa aggcggataa
     5461 acaaaaagcc gtcagcgata ttgtggcgct ggaaaacgcc ctggatatgt acaagctcga
     5521 caatcatcgc tatccaacca caaatcaggg acttgaatcc ttagtcgaag cgccgacact
     5581 gccaccgctg gccgcaaact ataacaagga aggttatatc aagcgtctgc ctgccgatcc
     5641 ctggggcaat gattatgtcc tcgttaatcc tggtgaacat ggtgcatacg atctgctttc
     5701 agcagggccc gatggtgaaa tgggaaccga ggacgacatc accaactggg gtttgagcaa
     5761 gaagaaaaag taagtcagcg atgaatcagc aacgcgggtt taccctgctg gagatgatgc
     5821 tggtgctggc gctggtcgct atcacggcaa gtgtggtgct cttcacatat ggtcgggagg
     5881 atgccgccaa cactcgtgcg cgcgaaacag cggcgcggtt taccgctgca cttgaattag
     5941 ccatcgaccg cgctacgctc agtggtcagc ctgtcggtat ccatttctct gattcagcat
     6001 ggcgcattat ggtaccgggc aaaacaccat cagcctggcg ctgggttcca ttacaagaag
     6061 atgccgcaga cgagagcaaa aatgactggg atgaagaact ctcaattcat cttcagccat
     6121 ttaaacctga tgacagcaac caaccgcagg tggtgattct ggccgacgga caaatcacgc
     6181 ccttctcttt gttgatggct aacgcaggga ccggtgagcc gctcctgacg ttagtctgtt
     6241 caggatcatg gcccctggat caaacgctcg ccagagatac ccgcccatga acaaacaatc
     6301 agggatgaca ctgcttgaag tcttactggc gatgagtatt ttcgccactg ttgcgctggc
     6361 tttaatgagc agcatgcaag ggcaacgaaa tgccatcgag cggatgcgta atgagacgct
     6421 ggcgctgtgg atcgcagata accagcttaa gtcgcaagat acattcaacg atgaaaacac
     6481 gtccagttca ggcaaagaga taattaatgg cgaagagtgg aactggcgca gcgatgtcca
     6541 ttcaagcaaa gacggcacgc ttctggaacg caccataacg gtgacgttac ccaacggtca
     6601 gaaaaccgct ctgactcgtt atcagagcat ccatgataga tccgggcagg cgcaagatga
     6661 ttaatcgcca acagggtttc acattgctgg aagtcatggc ggcactggcg atcttctcaa
     6721 tgcttggcgt gctggcgttt atggtttttt cgcaagcgtc tacgctacat caacgctcac
     6781 agaaagaaat ccagcagttc aatcaactgc aacgcaccat caccattctt gataacgatc
     6841 tcctgcaact ggtcgctcgc cgaaatcgga gtacggataa aatcatggtg ttgggtgaag
     6901 aggctatttt taccacgcaa agtcgcgatc cgcaggcccc gctcaatgaa gcgcaaacct
     6961 tacaaacggt tcactggtat ctgcgaaatc atacgcttta ccgtgctgtt cgtacttctg
     7021 tggatggcag gaaggatcaa cctgcccagg cgatgctgga acatgtcgag agctttcttc
     7081 tggaaagtaa cagcggggaa agcaaagaac atcctctctc agtcacgcta catctgaaga
     7141 ctcagcaata tggggcacta cagcgtcgtt ttgcattacc tgagcaatta gcaagagaag
     7201 agtcgccagc ccagactcag gccgggaata ataaccatga ataatgaaca acgcggcgtc
     7261 gcgctgctca ttgtattgat gctactggcg ctcatggctg cactggcagc agacatgacg
     7321 agcagttttc acggccagct tcagcgaacc cgccaggtaa accatcatct gcaacggcaa
     7381 tatgatattg aactggcgga gaaacttgcg cttgccagcc tcacccagga cgttaaagat
     7441 aacgacagac agaccacgtt gcagcagtac tgggcacagc cgcaacagtt gcagctggaa
     7501 gatggcaata cagtgaagtg gcagttacgc gatgcccagc actgttttaa tctcaatgcg
     7561 ttagcaaaaa tctctgacga tccgctggct tcccccgatt tccccgccca ggtattcagt
     7621 gcgctgctga ttaacgccgg tatcgatcgc ggaaacaccg atgagatcgt gcaatcaatt
     7681 gctgactata ttgatgtcga tgactcacca cgctttcacg gcgcagaaga taacttttac
     7741 caaagccaga cgccgccccg gcatagtgcc aatcagatgc tttttctgac cggcgaattg
     7801 cgtcagataa aaggcataac cgaaaacatc tatcagcggc ttatcccgta tgtctgcgtt
     7861 ctcccaacca cggaacttag catcaatctt aatatgctta cggagaacga tattccgctc
     7921 ttcagagcgt tattcctcaa taacatcacg gatgctgacg ctcgggtctt attacaaaaa
     7981 aggcccaggg aaggctggct aaccacggat gcatttcttt actgggcgca gcaggatttc
     8041 tccggcgtga agcctctggt agctcaggtg aaagggcatc tctttcccta tagccgctat
     8101 tttacgctga gtacggaaag tatcagcgac gaacaatccc aaggatggca aagccatatt
     8161 ttcttcaacc gtaaacagca gagcgcccaa atctatcggc gtacgctgca actctactaa
     8221 tcataaggat cgtgatgcct gaatcgttga tggtcatacg ttcttcctcg acgctgcgta
     8281 agcattggga atggatgacg ttctccgcag atagcgtatc ttcggtacat acgcccacgg
     8341 acgatcttcc gctggagtcg ctggccgatc agcccggtgc cggaaatgtt catcttttga
     8401 tcccaccaga gggcctttta tatcgttccc tcacgctgcc caacgccaaa tacaaactca
     8461 ccgcacaaac attgcaatgg ctggcagagg agacgttacc cgacaacacc caggactggc
     8521 actggacggt tgtcgataaa caaaacgaaa gtgtcgaggt gataggcatt cagtcggaga
     8581 aactcagtcg ctatcttgag cgtttacata ctgccggact caacgtgacg cgggttttgc
     8641 cagatggctg ctatcttccc tgggaagtag atagctggac tctggttaac cagcaaacaa
     8701 gctggcttat tcgcagtgcc gcacatgcct tcaatgaact ggatgaacac tggttacagc
     8761 atcttgctgc ccagtttccg cctgagaata tgctttgtta tggtgtggca cctcacggcg
     8821 ttgcagcgac caatccactt atccagcatc ctgaaatacc atcactgagt ttgtattctg
     8881 ccgatattgc attccagcgc tatgacatgc tgcatggtgt ttttcgtaaa cagaaaactg
     8941 taagcaaatc gggaaaatgg ctcgcccgcc tggcagtaag ctgtctcgtt ctcgccattc
     9001 tttcttttgt tggcagccgg ggcatcgcgc tctggcaaac gcttaagatt gaagatcaac
     9061 ttcagcatca acagcaagaa acctggcaac gttatttccc gcagatcaaa cgcacccaca
     9121 actttcgttt ttactttaag cagcaactcg ctcagcaata tcctgaagct gtgcctttgc
     9181 tctatcattt acaaacactt ctgctggaac accctgaact gcagttaatg gaggcgaact
     9241 atagccagaa gcaaaagtcg ctgaccttaa aaatgagtgc taagagtgaa gccaatatcg
     9301 atcgtttctg tgaattaaca cagtcctggc ttccgatgca aaaaacagaa aaagatccgg
     9361 tcagtggtgt atggacagta aggagctcag ggaaatgatt aaatcatggt gggcagaaaa
     9421 atccacatcg gaaaaacagc tcgttgcggc gctggcggtt ctctcgctcg gcgttttttg
     9481 ttggcttggg gtgataaaac cgatagatac ctacattgca gagcatcagt cccacgcgca
     9541 aaaaattaag aaggacatca aatggatgca ggatcaagcg agcacccatg gtttactcgg
     9601 tcatcctgcg cttacgcagc ccatcaaaaa tatccttctc gaggaagcaa aacgtgagaa
     9661 tttggctatc acgctggaaa acggtccgga caatactctg acaattcatc ctgtcacagc
     9721 tcccctcgaa aacgtctccc gctggctcac tacggcacag attacatacg gtattgttat
     9781 tgaagacctt cagtttactc ttgccggaaa cgaacagatc accctcaagc atttgtcctt
     9841 cagggagcaa ccataatgac aatgctactg ccgctcttca ttctggttgg ttttattgca
     9901 ggttattttg ttaatgccat ggcctatcac ctctcgccgt tggaggataa aacggcgtta
     9961 acgtttcgcc aggtattggt tcatttcagg caaaaaaaat atgcctggca tgatacagtg
    10021 cccctgatac tttgcgttgc tgctgctatc gcctgcgccc tggcaccttt cacgcccatc
    10081 gtgactggcg cactctttct ctatttctgt ttcgcactca cgctcagtgt tattgatttt
    10141 cgcactcagc tcctgcccga caaactcacc ttaccgctgc tctggcttgg cttggtattt
    10201 aatgcgcagt ctggattaat tgatttacat gatgcggttt acggcgcggt agctggctat
    10261 ggggtgctgt ggtgtgttta ctggggcgtc tggttagttt gtcacaaaga gggattgggc
    10321 tacggtgatt tcaagctact ggccgctgca ggcgcgtggt gtggctggca aacgttgcca
    10381 atgatactgc taattgcctc actgggtggc attggttacg ccatcgtttc acaacttctg
    10441 caacgccgga ctataaccac tatcgcattc ggcccgtggc ttgcgctcgg cagcatgata
    10501 aacctggggt atctggcctg gatctcgtat taaccgggag ggttctccct cccgacacgg
    10561 ctcaactttc ttcgcggatc tgtgcttgca gataattttg caggcccatc ttctgaatca
    10621 gatcaagttc cgtttccagc cagtcgatat ggccttcttc atcacgcaaa atttctatca
    10681 tcatatcgcg gctgacgtaa tcatgaacgc tatcggcata accaattgcc tcacgcaaat
    10741 tcttcgcgcc atccagctca agtgccagat cagaacgcag catttcctca acatcctcac
    10801 caatgttcag tttgcccagg tcctgtaaat ttggaagacc ttccagaaaa agaatgcgct
    10861 caatataacg atcggcgtgt ttcatctcat caatggattc atgatactcc acatcattga
    10921 gacgtttgag accccagttt ttaaacatcc gggcatggag aaagtactga ttgattgcga
    10981 caagctcatt tcccaacagt ttgttgagat aatttataac cttagtatca cctttcattt
    11041 tttgactcct tcgcttccac tctttgaagc gtagaacggc ttacgagtaa gtcaaaaaac
    11101 acactccgtt tatgcggact ccttaaactc cggcaactgc attaattcat cctccatcac
    11161 ttcacgcgcg gcccggacac atttaccgca ctgattgccg accggaataa atttttttaa
    11221 ttgctggaac gagtgagggg aaaactggcg cacagcctga cgaatttttt tatcgctgat
    11281 accattacaa agacaaacgt acatagagac tcccgtatac tttcttcaaa aataaatgaa
    11341 aatagttctt atttcaatta cggaaagact cttttttaag caaagaatag aaatatgaat
    11401 aaaaataatg ggctaccaaa agtagcccat tgacaaaaaa tgcggcgata ccggaaggta
    11461 tcgccaacac atttattgct tagtaaacgg cgcgaagatt tcgctaaatg cgtacttcgg
    11521 tgcctgttct ttagtcaggc cgtggaattc cggagacagg ttagtgccgc ctggcaggtc
    11581 gcgcgcgatt gaccagatac caaccatacc gagattacgc ttctgcgcat cctgcatgac
    11641 cagacgagca tcagagagat agaacacctc gccctgaacg tcgttcacgc caaccatcgg
    11701 cgtggtaccc atcatagcgt caatttctgc atcgctctta ttgggatgga ggcctttcaa
    11761 ttgtgaatgc aggttggcaa tcgcagacgt tgcacactta ccgtgaatgt tctggccttc
    11821 ggtatttgca gactgacaaa tcgcgttacc gtagtccatt gtcatcacgt tcacacccgc
    11881 cagctcaaca cctttcgctt tggcatcgct caggacattc atcccttccg gcgtcaggcc
    11941 agtcggcaga attggcaagg tgtaccagat agcaatatct ttgccctctg acttccattt
    12001 atcctgcact ttcttcacag caaggttacg acgttcaata gatgcctgat ccgcaaccca
    12061 ggtgccttcg atatcgaagt ccaggactct gaggttcagg ttatcaacga tgtcataata
    12121 atgctgcatc agatcgtcta cgttcttaca ggaagcagcc agcggagcgt tgttagcacc
    12181 accgatagac agcatcacat cgccgccagc ctcacgcaga gctttgattt tgctgtactg
    12241 agcgtaattc tgcataccgt aagcggtacc ccatgtcggc agacaggtgt tcgcatcttt
    12301 actcaccaca aacgccagcg tgaagtggtt gacgttatga ttcttagcca gcgcagccag
    12361 atccgggatc gtattcagcg taaagtcaac atacggtgca taaacgtgtt tcggccacgc
    12421 tttcttcggc gtacctacac gttctttggt tccggtccag tcaacaaaaa cacgccacgg
    12481 gttgctgtca gaaggagaaa ctttctgcac tttactctga gaaatgtagt tcgcaccgtt
    12541 aaagcgaatc agcgtatcgc tggcataaag cgtttctgga ttaaactgcg gcgcattatt
    12601 cagctcttcg ttgctatagc tctgagcctt acctaacggc ttccacgggc ggctattgct
    12661 accggtggcg ttttggttcg caaccagagc agggttgtcg ctttgcgtcc agaacagcgc
    12721 ttcataagcc tgcccatcaa caacaacgcg atcacctttc acgtacacgg tggaagcgga
    12781 ccatgctttc gccggttcgt atttcttcca cgggttatcg ccgcccggga caaagcccca
    12841 tgcgtcttga tcgacagagg ttttataatg tgcatcgtta taatctacga tgctgttcgc
    12901 gctgtacgct ttgctggcct ggaaaccatc cgcaacaatg acgccgccat tatcaatttc
    12961 acaggtagtt gggttaccgt actgactcat ttccgcagcg gtagctgtac gcacgagacg
    13021 ccatgggtta ttggagtttt ctgcactagt gccacgtggg caatcttcag aaccaaccca
    13081 ccaggcattc tgataaacat taccattgaa gataacatgg taaatttcgc taccttcctg
    13141 gccactccac gcctgcagtg agtaatcgtt actgctatcc gctggtgttg atggctcgtt
    13201 attatccgga gtaggagtaa ctggagttac cggtgtttcc ggagttggat caaccggagt
    13261 cacagcaccg ctattattat ccggcttaac ggaacaggaa cccggattac catactggct
    13321 gatttctgtt gccgttgccg cgcgaacata acgccatggg ttgctggcat cgttttcttt
    13381 cgcatcgcct gggcaatttg ccgcgcctac ccaccaggcg ttcttataaa tgccgccgtt
    13441 gtagataacg taccatgtag aagaaccttc ctgacctttc caggctacga cagcatagtt
    13501 gtcgtttgac ttatcagctg gagctggctg aacatcagga gccggtttaa cgtccggtgc
    13561 cggtttcaca tccggcgcag gctgtggtgc tgaagtacag gactgtggat tactggtttc
    13621 tgagatttcc gttgccgtag cggcacgaac ataacgccat gggttgctgg catcattgcc
    13681 tttcgcatca cccgggcagt tagaagaggc tacccaccag gcatttttat ataccgcacc
    13741 attaaagacg acataccagg tctgaccgcc ctgctgttta ttccaggcaa ctacagaaga
    13801 attagacgga acagtgccct gtgctggtgt agccgaaccg ccattagccg gcgtattgga
    13861 tgcaggcgta tttgaatttg aagaagatga gctgccgctc ttttcgcagg aaagtgtatt
    13921 gccaaactga ctaatttcag cagctgtcgc ggtacgcttt aaacgccatg ggttagttgc
    13981 atcatttgct tttgcttttc ccgggcaatt tgtagaagaa acccaccagg cattttcata
    14041 aattttgcca tcgaaaataa cctgatattt attaccacct tgttggttat tccatacttc
    14101 cattgccaat gctggcagag cggaacacac cagccccata ccaatcatag atttagtaaa
    14161 tatatttaat ttcattataa tcccttgtga cgtaaaaact gcaaaacaaa agtcctacgc
    14221 aggaattatt tacgttttac gagaaccctt caatatgaat taaattacgg cattaaaaat
    14281 aagaaaaaag cctgacaaa
//
LOCUS       DAPHCV010000112        14251 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.112, whole genome shotgun sequence.
ACCESSION   DAPHCV010000112 DAPHCV010000000
VERSION     DAPHCV010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.112"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(86..754)
                     /gene="artM"
                     /locus_tag="RH033_003714"
     CDS             complement(86..754)
                     /gene="artM"
                     /locus_tag="RH033_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HDV2992838.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLIVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(754..1470)
                     /gene="artQ"
                     /locus_tag="RH033_003715"
     CDS             complement(754..1470)
                     /gene="artQ"
                     /locus_tag="RH033_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HDV2992839.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1477..2208)
                     /gene="artJ"
                     /locus_tag="RH033_003716"
     CDS             complement(1477..2208)
                     /gene="artJ"
                     /locus_tag="RH033_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV2992840.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2226..2954)
                     /gene="artP"
                     /locus_tag="RH033_003717"
     CDS             complement(2226..2954)
                     /gene="artP"
                     /locus_tag="RH033_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HDV2992841.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3172..3687)
                     /locus_tag="RH033_003718"
     CDS             complement(3172..3687)
                     /locus_tag="RH033_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV2992842.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRGNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            3813..4136
                     /locus_tag="RH033_003719"
     CDS             3813..4136
                     /locus_tag="RH033_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDV2992843.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGDQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            4133..4963
                     /gene="amiD"
                     /locus_tag="RH033_003720"
     CDS             4133..4963
                     /gene="amiD"
                     /locus_tag="RH033_003720"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HDV2992844.1"
                     /translation="MRRVFWLVAVALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTLVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(4960..5973)
                     /locus_tag="RH033_003721"
     CDS             complement(4960..5973)
                     /locus_tag="RH033_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDV2992845.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(6072..7502)
                     /locus_tag="RH033_003722"
     CDS             complement(6072..7502)
                     /locus_tag="RH033_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HDV2992846.1"
                     /translation="MPQRILVLGASGYIGQHLVHTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPAHEQSDHLRARQATADILREAGVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPFPTRWISVWFLNVITSVPPTTAKALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRRTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKRIARLAEQSTD"
     gene            complement(7513..8514)
                     /gene="ltaE"
                     /locus_tag="RH033_003723"
     CDS             complement(7513..8514)
                     /gene="ltaE"
                     /locus_tag="RH033_003723"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HDV2992847.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSRAQLAEVAAHWRAFLAR"
     gene            complement(8551..10269)
                     /gene="poxB"
                     /locus_tag="RH033_003724"
     CDS             complement(8551..10269)
                     /gene="poxB"
                     /locus_tag="RH033_003724"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HDV2992848.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVMVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(10402..11370)
                     /gene="hcr"
                     /locus_tag="RH033_003725"
     CDS             complement(10402..11370)
                     /gene="hcr"
                     /locus_tag="RH033_003725"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HDV2992849.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(11382..13034)
                     /gene="hcp"
                     /locus_tag="RH033_003726"
                     /gene_synonym="priS"
     CDS             complement(11382..13034)
                     /gene="hcp"
                     /locus_tag="RH033_003726"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HDV2992850.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(13178..14077)
                     /gene="lysO"
                     /locus_tag="RH033_003727"
     CDS             complement(13178..14077)
                     /gene="lysO"
                     /locus_tag="RH033_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HDV2992851.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQKAALRVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         3456 a         3806 c         3754 g         3235 t
ORIGIN      
        1 ttgtccgtct tcattataag aatttatgca ctatgtaggc cggataaggc gtccacgccg
       61 catccggcat cagcacgcag ccaaattaat tccttcgctc aaacgccagt gctttacgct
      121 cgatcagacg catcatcagc gtcagcagac cgttaacaat caggtaaata atccctgccg
      181 caccgaacac catgacatcg taggtgcgtc cgtacaacaa ctggctgtat cccatcactt
      241 ccatcagcgt aatggtgtaa gccagagaag tacttttgaa caccagcacc acttcgttgg
      301 aataagaaga gagcgagcgt ttaaacgcgt acggcagcag gatcgccagc gtatcttttt
      361 tgctcattcc cagggcgcta caggactgcc actgaccttc cgggatcgcg cgaatcgcac
      421 cgtaaaacag ctgcgtggtg tacgccgcac tgttcagcga caacgcaatc agcgcacata
      481 accacggttc tgacaacaaa tgccacagtg ccggatactc ctgcaacgtc gggaactggc
      541 ccggtccgta ataaatcagg aagatctgca ccagcagcgg cgtaccggta aacagcgtga
      601 tatagccccg taccagccac actagcaccg gcgttttcag cgtcaggatg atggtaaaaa
      661 tcaatgccag aatcagtgcc acaatcagcg aggcaacggt tagcgtcagg ctggtgtgca
      721 gccctttcat cagttcgggt aaatactcaa acattagctg ggcctccgct caaaacgtgt
      781 cgcgcgcagg tcaatgcgtt tgagaatgta ctgactgagc agggtgatca ccaggtaaat
      841 cgccgccgcc acaatgtacc aggtaaatgg ttcctgggta cgggtagcga tgcttttggt
      901 ttgtagcatt aaatcattca cactaatcaa actgaccagc gcggtatctt tcagcagcac
      961 cagccactgg ttaccgagac caggcagcgc atgacgccac atctgcggca tcaccagacg
     1021 gaaaaagata gccgatttcg acagccccag cgcctgacca gattcccact ggcccaccgg
     1081 caccgctttc aacgcgccgc gcagcgtttg cgaggcatag gcggcataca gcagcgacag
     1141 agcgatgaca ccacaaagga acgggctaac gtcgaagttc tcaatgtcca tctgcactgg
     1201 gatctgcacg aacccaagat tgatagtgaa gccatccgaa agcgtcagca gcagctgcga
     1261 ggagccaaaa tagataaaca gcaccaccag aatttctggc aggccacgca gaatggttac
     1321 cagcgctgaa cctgcccacg cgacaggacg ccattttgca gactcccata ccgcaaagaa
     1381 catcgccagc gccagcccga caatcaatgc acaaacggca aggccgacgg tcatcccggc
     1441 ggcgcttgct aaaggaaaaa attcattcat caggaattac ttctggaacc atttgttata
     1501 gatggtttcg taagtgccat ctttcttcac tttttccagc gcagtgttga gtttctgctg
     1561 cagttcagtg ttgccctggc gtactgcgat gccgaggcca gtaccgaagt aatctttatc
     1621 ggtcacttta tcgcccactg ccgccagttt cgggttgtct ttcagccact cggtgaccac
     1681 tgcggtgtca ccgaatacgc tgtcgatacg accgttttgc agatccagtt ttgcgttctg
     1741 gtagctgtca tacggaacgg tggtgatttc cgggtgctta tccataatga acttctggtg
     1801 tgtcgtcccg ttctgaacgc cgactttttt gcctttcagc tgatcaacac tagtgtattt
     1861 accctgctga cccacaaaca gggcagagtt gtcatagtaa ggggtggtaa acagcacctg
     1921 cttttcacgt tctggggtga tatccatccc ggccatcact gcttctacgc gacggaattt
     1981 caggcttggg atcaggctgt caaacgcctg gttagagaaa gtacaggttg catcaatctc
     2041 tttacacagc gcctgtgcca ggtcgacgtc aaaaccaacg atctggttgt ttgcatcaat
     2101 cgattcaaac ggaggatagg aggcttcggt agcaaaacga atggtttcgg cagctgtggc
     2161 ggaaagacta aaacctgcaa ttaacgcggc aatcagaact tttttcattg ttgttatccc
     2221 gaatcttagt gagagagata gtttttaaat gcttcggttt gcggctcggt aaagcagctc
     2281 gcgtcgcctt gttctacgat atgaccgttt tccatataca ccactcggct ggcggtttta
     2341 cgcgccactt caacttcgtg ggtgacgatc acctgggtaa tattcgtttc tgccaactca
     2401 cgaatgatgc tgacgatttg tgccgtaatt tccgggtcca gtgcggcggt cggttcatcg
     2461 aacagcagta cctgcggttc catcatcaac gcacgggcaa tagcaacacg ctgctgctga
     2521 ccaccagaaa gatgcagcgg gtaacgatcg ctataaggtt tgagacgcag acgttccagc
     2581 agtttttctg cacgggccag cgcctgatct ttgctcaacc ccagtacacg gcagggcgct
     2641 tcaatcaggt tttgctgcac ggtcagatgc ggccacaggt tgtattgctg aaacaccatg
     2701 ccaacgttac gacgcaaatc gcgaatcgct ttgtcagagg gtgttttggt gaaatcgaaa
     2761 tggttgcctg caatgttgag cgtaccggag cgcggcatct caagcagatt gagtacacgc
     2821 agcagcgagc ttttacccgc gccgctgggg ccaagtaaca ccagcgtttc gccctgtggg
     2881 caatccagcg tgatatcgaa cagcgcctga tgcgcgccgt agaagcaatt aatgccgttt
     2941 aattgaatac tcattgacac tcgtatactg gcagtctgat agctattgag gtcgaagata
     3001 gtacctttga cagaataatt atgcaatatt tctgctttaa aagttaaagg caaagcgcat
     3061 tattcaataa acatagcaca aaataacggg ggcggtggtc ggcgagcata aatgtcggca
     3121 ttcctcacga aatgccgtac aatttacggg gtttattggt tgatcaagac gttagcgatt
     3181 ctcgatggac tgacggagcg tacccgctgt ggcatgaacg ctaccgccta agtagcgcac
     3241 atcgtcgatg acccaacact ggccttcctg aatcattaac acttcatcct gccagccctg
     3301 gtcaccctgc ttgagatcca cgcgcaatgg aatgttacgg gcatcacgat tagggatagt
     3361 cgatgcactg gcaacgtggg cgctgtctgg caaggtggtt cgactggaga acggatcgct
     3421 gcttaacaat tcacgatgac tattatcgcg gctggcatcg ctaagtaggg ttgccagttt
     3481 gtcactcaaa taggggcgca gagcggtaat gtcattgccg cgatgttgaa tgcggtagtc
     3541 atagaactgt tgagccacgc tatcaggccc cccctggaca caggaaccgg tgcgggggcc
     3601 gttatcttta taagctggcg tgacggtggt acaggcactg agtagcagtg cgcaagggat
     3661 aagcatggtc aatttgctat agcgcatgat gatttcctta taagcgaaca ccttgaaagc
     3721 gttctacgat aataatgata tcctttcaat aatagcgtat cagtccgata atgctttgaa
     3781 gatcgaaggc ttagcaaaca aggagatcga tcatgcaatt ttctacaacc ccaactctgg
     3841 aaggccagac catcgtcgaa tattgcggtg tggtgaccgg cgaagcgatt ttaggtgcca
     3901 atattttccg tgatttcttt gccggtatcc gcgatatcgt tggcggacgc tccggggctt
     3961 atgaaaaaga attgcgtaaa gcgcgggaga tcgcctttga ggagctcggc gatcaggccc
     4021 gggcgctggg ggctgatgcc gtagtcggta ttgatatcga ctacgaaacg gtcgggcaaa
     4081 atggcagtat gctgatggtt agcgtcagcg gtacggcggt gaaaacgcgt cgatgagaag
     4141 agtcttttgg ctggtcgctg tcgctctgtt actggcaggg tgtgcaggcg aaaaaggcat
     4201 tgtcgagaaa gagggatatc agcttgatac ccgacgccag gcgcaggcgg cgtatccgcg
     4261 cattaaagtg ctggttatcc actacaccgc agatgatttt gatagctcgc tggcgacact
     4321 gaccgataag caggtcagct cgcattatct ggtccctgcg gtaccaccgc gatacaacgg
     4381 taaaccgcgc atctggcaac tggtgccgga acaggaactg gcctggcatg cggggattag
     4441 cgcctggcgc ggggcaacgc gtcttaacga cacctctatt ggcattgagc tggaaaaccg
     4501 tggctggcaa aaatcatccg gagtgaaata ttttgccccg tttgaacagg cacagattca
     4561 ggcgcttatt ccgctagcga aagatattat tgcccgttat cacatcaagc cggaaaacgt
     4621 agtggcgcat gcggatattg cgccgcagcg caaagacgat ccggggccgt tattcccctg
     4681 gcaacaactg gcgcagcagg ggattggtgc ctggccggat gcgcagcggg tgaactttta
     4741 ccttgccggg cgcgcgccgc acacactggt agataccgca tcattactgg agcttttggc
     4801 gcgctacggt tatgacgtta aacctgatat gacaccgcgc gagcagcggc gcgtgattat
     4861 ggcgttccag atgcatttcc gcccgacgtt atacaacggc gaagcggatg cagaaactca
     4921 ggcgattgcc gaagcgttgc tggagaaata cgggcaggat tagcgcggca gttttccgtg
     4981 gtcgcgtagc caggcggcag ttttctcgat accttcatcc agggtgatga ccggctgata
     5041 acctaactct tcctgcgcgc gcgtaatatc cagcgtaaag tcaaaattca acttggagac
     5101 gccgtagtgg gtcagcggcg gctcttttgc tgacttgcgg cctaaacgct ccatgctgcg
     5161 ggcgatcata tccagcatcg ggtaggggac ggaacgaata cgacagtcaa tattcaactc
     5221 gtcgatcagc ttctgcacga tgctgcgcag tgtgcgatgc tcgccgttgg tgatgttgta
     5281 cacacgcccg gaaggtagct tatcgcaggc ttcctggctt gccagccaca tggcgtgcac
     5341 ggcattttca tagtaggtca tatcgaccag cgcactgccg ccatgcggta acagaatgct
     5401 gccgtagtgg tgcatcatat gcgccagacg gggaataaag actttatcgt gcggtccgaa
     5461 cagactttgt gggcgcagaa tagtaaagcg cgtttgtgga ttcgcctgcg aaagcatatt
     5521 gatcacctct tcgctggctg ctttgctgcg ggcaaactcg ttggcgaagc ggtgagggcg
     5581 aaaatcttct ttaatatcgc gatggtggtg ataatcgaag tacagggagg gggaagagat
     5641 atgaataaag ttacgcacac cccaggcgac agcccattca cccaggcggc gagtggcgcg
     5701 aacgttagcc agatcgaaag cctgttgtgt cccccagggc gaggtaaagc tggagcagtg
     5761 ccacagcgta tcaatgcccg cgagcatcac tttagcttgt gatgagacca gctcggtcag
     5821 atccgccgga acaaactctg cgcccatttt ttccagcaat ttacccattg cctcgttgcg
     5881 accggtcgct cgcacgctga tgcctttctg gcataaaaac tctaccgcgt ttcgacctaa
     5941 gccgctggtg gcgccggtaa ccagtacctt catatcaatc cactgttgtt gagaaaataa
     6001 cgtgcgcatt cttccgtgat ttcccccatg atgcaatggg aaacatgaaa gaataacgca
     6061 ggttttgtcg attaatctgt gctttgttct gccagtctgg cgattcgttt tgccattccg
     6121 cgaaaaataa acagatgcgc ggggatcatc aataaccagt aaaacagccc cggcataccg
     6181 tgcggatgcc agaatgcgcg aacatcgata gtacgatagt cacctttatc ttccagggta
     6241 aaacacagtc gtcccagccc cggcgctttc atgccaaata acagcgtaag ttgtttttcc
     6301 ggttcaacga caatcacttt ccagctatcc accgcatcgc cggtctgcaa atattcacgc
     6361 tccgggcggc ctttcgccag cttatgaccg atcgcgcggt ccatcaacgc ccgggtttgc
     6421 cacaaaatat tgccaaagaa ataacgctct ttaccgccga tttgattcac cacctgccat
     6481 aaagccgcaa ggctggcgga cgttttaacg gtaaaccccg cctgtttggc gaaataaccg
     6541 tactccggtc gccagcgggc aaaggcctga gcgtcgtatc cccagtcgct ggagttgacc
     6601 agcttttctt cctctttcag ggtgcgacgt accgcgtcat cgaaagcaat cagccgttgt
     6661 gggatgagtg cacgtaacgc ggtgtcatcc gccagcagat catgtttcag cccctgaatc
     6721 aacgctttgg cggtggtggg cggtacggaa gtaatcacat tgagaaacca caccgaaatc
     6781 cagcgggtcg gaaaggggat ggggatcaac cagcggcgct taccgctcac cgccataaaa
     6841 tgttcaaact gttgctgata actgagcact tctggtccgg cggcttcgaa gatgcgatgt
     6901 tcgctggccg gatgatctaa cagcgccacc agatagtgca gcaagttttc cagcgcgatg
     6961 ggcgtggtgc gtgaacgaac ccagcgtggc ggcgttaaca ctggcaggtt gtagaccata
     7021 tcgcgcatga cttcgaacgc cgctgaacct gcaccaacga ttattccggc acgcaattcg
     7081 gtcaccggga cacccgcttc acgaagaata tccgccgtag cctgacgggc gcgcagatgg
     7141 tcagactgct catgtgccgg ggcctgcaac gaactgagaa agattaattg cttaactggg
     7201 acttcacgta gcgcatcgcg gacgttgaga gccacctggc gctcctgagc gataaaatcg
     7261 ccgccttcgc ccatgctgtg caccagaaaa tagaccgtat cgatatcctg caacagggcc
     7321 ggaaggttat ccggccagct gagatcgact ttatggcaac tgacgtttgc cagttgcagt
     7381 tttgcaagcc tgtcgacatg acgtgccgcc gccaggatct gatgcccctg ctggctgagt
     7441 gtgtgcacca gatgctgacc aatatagcca ctggcaccga gaactaaaat gcgttgcggc
     7501 acgtctccct ccttaacgcg ccaggaatgc gcgccagtgg gcggcgactt ccgccagttg
     7561 tgcacgcgag acgtcaagat gggtcaccag gcggacaatc ggcgaggcgt taatcagcac
     7621 gtttctcgct ttcatgtatt cgcctaacgc ggcagcattt tcttccccga cgcgaacaaa
     7681 cagcatattg gtgtcctgac gcatcacatc cgcgcctgct tcacgcaact gctccgccat
     7741 ccaggcggcg ttgtcgtggt cttcctgtaa gcgcgcgacg ttatttttca gggcatacat
     7801 cccggcggca gccagaatgc cggactggcg catcccgccg ccggtcattt tccgccagcg
     7861 gatggcgcgt ttaatgtaat cacgattacc gacgagtaat gaaccgactg gcgtcccaag
     7921 acctttcgac aggcaaatgg tgaacgaatc acaatattgc gtgatctctt tcagttcgca
     7981 gccgtaagcc accacggcat taaagatgcg cgcaccgtca acatgcagcg ccagattgcg
     8041 ctcgcgagta aattcccatg cttctttcag gtattcccgc ggcaacactt tgccgttgtg
     8101 ggtgttttcc agactgagta atttggtgcg ggcgaaatgg atatcgtcgg gtttgatctt
     8161 catcgccact ttatccagag gtagcgtgcc gtcggcagcc gcgtcgatgg gttgcggctg
     8221 aatactgccc agcactgctg cgccaccggc ttcaaacaga tagttatgcg cggcctgacc
     8281 gacgatatac tcttcgccgc gttcgcagtg actgagcaga gcgaccaggt tggcctgagt
     8341 gccggtaggc agaaaaatgg cggcttcttt accggaaagc tctgctgcat agtcctgcag
     8401 agcattaacg gtagggtcgt ctccgtaaac gtcgtcccca accggggcgg ccatcatcgc
     8461 ttcgagcatg gcgcggctcg gtcgggtaac ggtatcactg cgtaaatcaa tcatggcatg
     8521 tccttattat gacgggaaat gccacccttt ttaccttagc cagtttgttt tcgccagttc
     8581 gatcacttca tcaccgcgtc cgctgatgat tgcgcgcaac atatacaggc tgaaaccttt
     8641 ggcctgttcg agtttgatct gcggtggaat ggctaactct tctttggcga ccaccacatc
     8701 caccatcacc ggaccgtcga tggagaaggc gcgttgcagg gcttcatcga cttcagaggc
     8761 tttttctaca cgaatacccg taatgccgca cgcttcggca atgcgggcaa agttggtgtc
     8821 gtgcagctca gtaccgtctg tcaggtagcc tccggctttc atctccatcg ccacaaagcc
     8881 cagcacgctg ttattaaaga cgataatttt caccggcagt ttcatctgca ctactgagag
     8941 gaaatcgccc atcaacatgc taaaaccgcc atcgccgcac atggcgacca cctgacgttc
     9001 cggctcggtt gcctgcgcac ccagcgcctg cggcatggcg ttagccatcg aaccgtggtt
     9061 aaacgaacct aacaggcgac gcttgccgtt catttttaga taacgtgccg cccacaccgt
     9121 tggcgtacca acgtcacagg tgaaaatagc gtcatcggcg gcaaaatgac taatttgctg
     9181 cgccagatat tgcgggtgaa tggctttctc gctcggttta gctaaatcgt ccagcccttt
     9241 gcgggcgtcg cggtaatctt ccagcgcttt atccagaaac ttgcgatcgg ctttttcttc
     9301 caccaatggc agcaatgcac gcagagtcga cttgatatcg ccgaccagtg ccatatccac
     9361 cttgctgtga gcgccgatgc tggctgggtt gatatcaatc tgaataattt tggcatcggt
     9421 cgggtagaac gcacggtagg gaaactgagt accgagcagc actaacgtgt cggcgttcat
     9481 catggtatgg aaacctgacg aaaagccgat taatcccgtc attccaacat catatggatt
     9541 atcgtattcg acatgttctt taccgcgcag ggcatggact ataggcgctt taattttccc
     9601 ggcaaactca actaactctt tatgcgcccc cgcacagccg ctgccacaca tcagggcgat
     9661 attgctggaa taacgcagca gctgcgccag tttgcgtaac tcttcttctt ccggtgttac
     9721 taccggctgt ggcgcatgat accagtgggt agttgcccct tctggcgcag gttttaacgc
     9781 cacgtcgccc ggtaacacaa caacggaaac gccacggtta agcaccgctt tgcgcatagc
     9841 aattgccagc acttgtggga tctgctctgg gctggaaacc agctcgcaat agtgactaca
     9901 ttcgcggaat agctcttgtg ggtgggtttc ctggaaatag ccgctgccaa tttcgctgga
     9961 gggaatatga gcggcaatcg ccagtaccgg aacgtgattg cggtggcaat cgaacaggcc
    10021 gttgattaag tgcaggttgc cgggaccgca cgatccggca cagaccgcca gttctccgct
    10081 aagttgtgct tcagcgccag cggcaaatgc cgccacttct tcatgccggg tggacatcca
    10141 ctcgatggtg cccatgcgat taagactgtc actaagaccg ttcagagagt cgcccgtgac
    10201 tccccagatg cgtttcacgc ctgccgattc gagtgttttg gcgatataag ctgcaaccgt
    10261 ttgtttcatg gttctccatc tcctgaatgt gataacggta acaagtttag ttcatctgac
    10321 ggagggggaa gggatgggag aaaaaggagg cactaacggt taaatagccc gatgaaagga
    10381 atatcatcgg gcatcaggcg attatgcgag aaccaaatcc ccctgcggat ggcaggagca
    10441 ggccagtaca taaccttcag cgatttcggc gtcggtcagc gtcattgtgc tgctcaccgt
    10501 atattcaccg gaaaccactt tcgtcttaca gcagccgcaa acacctgcgc ggcaggcggc
    10561 gacaaccgga acgttattgc tttccagcgc ctccagtagc gtggtgccaa ccggggcgta
    10621 aaattctcgt gccggttgca gtttggtgaa tttcagaccg ctggtcgccg cttccgctac
    10681 tggggtgaag aatttctctt taaagaaacg cgtcacgccg agcgctttca cttcctgctc
    10741 tacccaatcc atatatggag cagggccaca ggtcatcacg gtacgtgagg ctaaatcagg
    10801 gacacctgcc agcagttcgc gagtgagacg accagcgata aagccttcgg taacgttatt
    10861 ttccgccacc agcgttaccg gatagttacg ccactcatcg gcgaaaatca catcctgcgg
    10921 cgtacgcacg ttgtagatca cctgcacatc ggcctgtgga cggttcttcg ccagccagcg
    10981 acgcatcgac ataatcggcg taacgccgca gcctgccgcc agcaacagga atttatcttc
    11041 tgctttatca tcgcaggtaa attcccccat cgcgtccgaa agccagagat aatcaccgcg
    11101 ttttacatcg cgcgtcagcc actgggagcc gacaccgtca tcaatccgcc gcacagtcag
    11161 ggtgatgtat tcactcacgc ctggcgtgga ggaaatggtg taagcacgca gcgtttccgc
    11221 tgagttacgc acgctgacca gtgcatattg cccggcgcga tatgggtagt aatcgtggca
    11281 aatcagggaa atcgtccaca catccggcgt ttcttgcgta atgtgatgaa cctgcatccg
    11341 ccacgggcat tgattcgttg gcatcgtcat cgacaaactc cttacgcgct caacagttgc
    11401 ttcatgtctt cttcaacagt ggtgatagaa cgcaggccga atttctcgtt cagcaccgcc
    11461 agcaggtctg gtgtcaggaa accaggtgca gtcgggccgg tgacgatatt tttcacaccc
    11521 agagaaagca gcgtcagcag aatgacaatc gctttctgtt caaaccagga gagcaccagc
    11581 gacagcggca gatcgttgac tccgcagccc agtttctctg ccagcgttac cgccagaata
    11641 attgctgagt aagcatcgtt acattgacca gcatctacca gacgcggcag accttcgata
    11701 tcgccaaact caagtttatt aaagcgatat ttaccgcaag cgagggtcag gatcaggcag
    11761 tcatccggca cgctggtggc gaaatcggtg aaatagtgac gctcgccgcg tgcgccgtca
    11821 cagccgccaa gcaggaagat atgacgcagt ttttcacggc tcaccagatc aatcagcgta
    11881 tcggctgcgc caagcagtgt ctggcgacca aagcccacgg tgatcaggtg cggaatttcg
    11941 ctgtacggga agcccgccat ctgttgcgcc tgggcaatga ctgcagagaa atcttcgcca
    12001 tccagatgac gcacacccgg ccagccaacg atgctgcggg tccagatacg atcgtcataa
    12061 gcgcctacgg ttgggtcgat gatgcagttc gaggtcatca cgatggggcc tgggaaacga
    12121 gcgaactcca cttgctgatt ctgccagccg ctgccgtagt taccgaccag atgcttgaat
    12181 ttacgcaact ccgggtagcc atgcgcaggc agcatttcgc cgtgggtgta aacattaacg
    12241 cccgtgcctt cggtctgttc cagcaggttg tagagatctt tgagatcgtg accggaaatc
    12301 agaatgcatt tacccgccgt cgctttgacg ttgacctgag ttggcgtcgg gtgaccgtat
    12361 ttaccggttt cgcctgcgtc cagaatgctc atcactttga agttcatctg gccgatttcc
    12421 attgaacact caagaagcgc gttcatatcg gcaggccagg tccccagcca cgccatgatt
    12481 ttatggtact gggcataaat atcgttgtcg tattgaccga gaacatgcgc gtgttccata
    12541 taggccgccg cacctttcag gccatacagg cacagcagac gcaggccgag aatgttttcg
    12601 ccaatcgccg ctttatcttt gttaggggta aattctgctg cctgacgttg cagctcgccg
    12661 agatcgtcgc tcaccagttg caggtcagcc atcgggttat cgacgcgggc gttggcatct
    12721 acagccaggc attgtgcttt cagcgcctcg cgcagggcaa tcgcttcacg agcgtagccg
    12781 acaatacgcg gagaatcgaa gttaacgttg gtcagggttg agaaaaaggc acgtggcgca
    12841 aagctgtcta catcgtggtt gatgatgccg tattcacgtg ctttcactgc ccaggccgaa
    12901 agcccttgca gcgccgcgat gagtaaatcc tgaaggtcag aggtttctgc cgttttacca
    12961 cacatcccct gcgcgtatga gcagccgttt cctgccggag tacggatagt ttgttcacat
    13021 tgcacacaaa acatgatcac accttttaaa gttatattta atatacatgt ttaaggttaa
    13081 gacgcttaac gcggggataa aagagatttt taatgcaact ttaagggaga ttgatttagc
    13141 gcaattttgg cggcagagat ctaccgccag agaggtatta tgcagagaaa aaggcgatga
    13201 gaatcggcac taacagacta agaataaaac cgtgaacaat agccgccggg accatatcca
    13261 gcccgcctgt acgttgaaga acgggcaggg tgaaatccat cgacgtcgca ccgcataagc
    13321 ccagtgcagt agagcggcta cggcgaatca gcccagggat taacataata gcaatcagct
    13381 cacgggccag atcattaaaa aacgccgcgc tcccgattac cggaccaaaa gattcggtca
    13441 ataaaatacc ggaaagagaa taccagccga aaccggaggc cattgccagc gcggtattga
    13501 tgggaagatc aagaataaag gcgttaatta aaccaccaat taatgaactg gcaaccacca
    13561 ccacggcgac aatcattccc cggcgattaa ggacaatctg ctttaaggtc atgccattat
    13621 tgcgcaactg aataccgacg aggaaaagta gcagaattaa ggtgtattca ctggcttcgg
    13681 tcgcgtgttg taagaaagcc agtccactca ggccaatggc aaaaccaatc actactacgc
    13741 cgcacagttt tagcgactcc agcgccatcg caatacgcga cgggagtttt tcttgctgat
    13801 ggtgattgcg ccatggcagg ccgcgctcca gccacatcag ggcggcaata ttacacagta
    13861 aaataacggt aatactgaca gcagaataat gcagaatcgc caacaggtta ctggcgaggt
    13921 tatcgagaaa cgccagactg atacccataa aaaagagaat aaggtaaacc atccagctta
    13981 atagctgatt aataactctt aatgcagctt tttggcgaag cggaatgagg taacccacaa
    14041 tcaggggaac cagaatgatt aacagcccag aaaacatgaa aacccagtcc ttgcaaagat
    14101 gaagtcgaaa tgcgcgatga cacactactg aaagcggaag gacgagtaaa gttgcaatta
    14161 aaaggaaatg ttatgcatca gagggagatt tttaattcta tatgagtgtt tagcattgtt
    14221 gcgctccttt catgtgcaat gccatataaa g
//
LOCUS       DAPHCV010000113        14249 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.113, whole genome shotgun sequence.
ACCESSION   DAPHCV010000113 DAPHCV010000000
VERSION     DAPHCV010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.113"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..550
                     /locus_tag="RH033_003728"
     CDS             <1..550
                     /locus_tag="RH033_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992852.1"
                     /translation="YINKDNIKVSYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQ
                     RITTFFQTSPDVKNREIKLEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLD
                     EIQTPSAINSELYTKMIYLTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSI
                     VISVMFCLVKKMVDEYPQNSGQ"
     gene            complement(791..1486)
                     /locus_tag="RH033_003729"
     CDS             complement(791..1486)
                     /locus_tag="RH033_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HDV2992853.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLTQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGIENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1479..2906)
                     /locus_tag="RH033_003730"
     CDS             complement(1479..2906)
                     /locus_tag="RH033_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HDV2992854.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQENKNG"
     gene            complement(2917..3636)
                     /locus_tag="RH033_003731"
     CDS             complement(2917..3636)
                     /locus_tag="RH033_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HDV2992855.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(4164..5018)
                     /gene="rclR"
                     /locus_tag="RH033_003732"
     CDS             complement(4164..5018)
                     /gene="rclR"
                     /locus_tag="RH033_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HDV2992856.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFVAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            5244..6569
                     /locus_tag="RH033_003733"
     CDS             5244..6569
                     /locus_tag="RH033_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HDV2992857.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADIPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPAH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            6678..6914
                     /locus_tag="RH033_003734"
     CDS             6678..6914
                     /locus_tag="RH033_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV2992858.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     NAIADMARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            6926..7519
                     /gene="rclC"
                     /locus_tag="RH033_003735"
     CDS             6926..7519
                     /gene="rclC"
                     /locus_tag="RH033_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HDV2992859.1"
                     /translation="MERYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQHLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSARDILKQRSNESSSTLKTEY"
     gene            complement(7679..8548)
                     /locus_tag="RH033_003736"
     CDS             complement(7679..8548)
                     /locus_tag="RH033_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDV2992860.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            8797..9654
                     /locus_tag="RH033_003737"
     CDS             8797..9654
                     /locus_tag="RH033_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV2992861.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKSFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMSKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
     gene            complement(9779..14026)
                     /gene="fdeC"
                     /locus_tag="RH033_003738"
     CDS             complement(9779..14026)
                     /gene="fdeC"
                     /locus_tag="RH033_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
                     /protein_id="HDV2992862.1"
                     /translation="MSRYKTGHKQPRFRYSVLARCVAWTNISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKNFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSENDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKTSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAITAEVNYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTVSLGLVVSKATHGLK
                     NVQWEAPSLLAAGGKITGQGNQWQVTLPAYQAGKDNYYAISAIAYDNKGNASKRVQTE
                     VVISGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDDMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     LVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDANDNPVTGLNPDAPSLSGAAAAGSTASGW
                     TDNGDGTWTAQISLGTTAGELEVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLALSASLTGAASEGATVSCWTEKGDGSYVATLTT
                     GGKTGELLVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTELTFTV
                     KDAYGNTVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKVDIELMSTVAGELEIEASVKNSQKTVKVKF
                     KADFSTGQASLEVDAAAQKVANGKDAFTLTATVKDQYGNLLPGAVVVFNLPRGVKPLA
                     DGNIMVNADKEGKAELKVVSVTAGTYEITASAGNDQPSNAQSVTFVADNTTATISSIE
                     VIGNRAVADGKTKQTYKVTVTDANNNLLKDSEVTLTASPENLVLTPNGTATTNEQGQA
                     IFTATTTVAATYTLTAKVEQADGQESTKTAESKFVADDKNAELAATSDVHSLVADGVT
                     TATLTVTLFSANNPVGGTMWVDIEAPEGVTEADYQFLPSKNDHFASGKITRTFSTNKP
                     GTYTFTFNSLTYGGYEMKPVTVTINAVPADTEGAEEK"
BASE COUNT         3702 a         3600 c         3123 g         3824 t
ORIGIN      
        1 gtatatcaat aaagataata taaaagtgag ttacagccta aaaataaacc agacaacacc
       61 aggcatactt gttagctgtg atagcaataa taactttgcc tgtcagacca caatgactga
      121 agatgttatt cagcgaatta ctacattttt tcaaaccagc ccagatgtca agaacagaga
      181 aataaagctg gaatggtcag gagataagag agatttacca actgctgaag cggaaatatc
      241 tcgcgtgcag gcgtctatca tcaaatggta tgcgtcagaa taccataatg gcaggcaagt
      301 tctcgatgag atacaaacgc cttcagcaat taacagtgag ctttatacaa aaatgatata
      361 cctgaccagg aactggtcac tgtatccaaa cggtgatggc tgtgtcacta taagctcacc
      421 agaaataaaa aataaatacc ctgctgccat ttgcctggct ctgggatttt ttctaagcat
      481 tgtgatttct gtaatgtttt gccttgtcaa aaaaatggta gatgaatacc cacaaaactc
      541 tgggcagtaa taaacagcat tctgacagag agtgcctgta taaaactata gtgacaatga
      601 ccgggaaggg agcccgtctt caggaatgga ttcctgtctt tttcactgta aatatcctgg
      661 cattattaat gtcaggatat ttacagaaga gcagcataac catgacggaa cggggttaag
      721 tcaggttgtc cttaacgcaa ttcacaacct cgtgccgcct tcaggtttat gtccagactt
      781 catatctctc tcagcaatcc tcaataatca gatacaccgc tttcaccgga ccatgaaccc
      841 cgacgacttt gataagctca atgtccgccg ttgaactggg accactgatg atgttaatac
      901 atgacggcat tcgttcaccg gcctgcgctt tctgatgcaa tttttctgcg agttgcgcta
      961 cacgcggcag gattgtgctt ttacgcagga taaaaagaga agattccggg agcaggctca
     1021 atgaacgccc gcgcccggcg gcggaaaaaa gaaccacgcc tcccgattcg gttaaaccat
     1081 attcagcata tacgacgccc actttagcct gctctgcttg tgatatgttc tcaatacctt
     1141 tcgccggatc ccaaacaacg gcattgcatt cctgctgcaa acgttcgcta atccccaatt
     1201 cctccagcct cgtgtcacca ctaatcacga ccgactgatc tcccagctct ttacacagac
     1261 gtattgcagc ttctgccgcc ttcgcctcgc tggtcagttc acagcgcgtc aacataacat
     1321 cgctggcaaa ctggataaac gcgtcacagc gctgctgttg ggtaagttgg gtaagccgct
     1381 cgttagcata gttgttaagc ggcgcatctt ctgcttgcgg ttcaagtcgc agcgggcgac
     1441 ccagtgcctg agcaacgtta ttcaaaaatt cgctccgatt atccattctt attctcctgc
     1501 gcctgatgtt tcttaaacca actacggaaa ctctctccgt cagcttcagg aagatcgcgt
     1561 gcttccatcc agtcgctaat cgcgccaaat ttgagtggtg ttttgccgcc attgataaac
     1621 cagcttgccg catgagcacc agccatcatc ccgactttcc acaatcctgg atgactattg
     1681 gcataagcga acatttttat cgcccgttgc tctgcttttg cggtgatccc tttttcagcc
     1741 atcacccgac gatgacgcaa aatcagtttt gacagcggaa tacgcaccgg acacacgctg
     1801 tcacaagcgg tgcataaaga gcaggcgtag ggtaaatctt taaaatcttt atagccgcca
     1861 agtaacgggg aaatcaccgc accaattggc cctggataaa tagagccata tccatgaccg
     1921 ccaatatggc gatatgccgg acaagtattc atacaagccc cgcaacgaat acagcgcagc
     1981 acatcccgaa attcagaggc cagcacctca gaacgcccgt tatcgacaat aaccagatga
     2041 aactcttcag gaccatcaac gtgccccgct tcgcgcggtc ctgtcaacca ggtgttgtat
     2101 cccgtcaaac gtgcaccaac ggcactgcgc gccagcatgg tgatcaatac atctacctcg
     2161 gcaaacgtgg gggcaatacg ctccattccc atcactgcaa tatgcgtttt aggcagcgtg
     2221 gtacacattc gcgcattacc ttcattggtc accaggcata ccgaaccggt ctctgccacc
     2281 gcgaaattac agccggtaat acctatttca gcactgagga aatcttcgcg gattttttgc
     2341 cgaatgaata aggtcatcgc ttcaggcgtt tccggcccct catagcccag acgttcgtgt
     2401 agcactcgac ggatctgatg gcgatcttta tgaattgccg ggaccacaac atgagagggc
     2461 ggatcttgat ccagctggag aatgtattca cccagatcgg tttcaatcac ctgaatgcca
     2521 gcatcctgca acacatgatt gacaccaatc tcttcggtca ccatcgattt agatttcacc
     2581 accttccggg catttttgcg ttgggcaacc tgtaaaatgt agcgggtagc gtcttctttg
     2641 gtttttgcaa aatagacgtg accgccgttt tgcgtcactt tttctgagag ctggtacaga
     2701 taagcgtcga gattactcag aacatgatca cgtatctggg cggcccgatc gcgccactcc
     2761 tcccagtgcc ccaattcatc gaccattttt tgccgatttg ccccaatacg ctgctgcgcg
     2821 tttgccaccg ctttgcgcat gatcggatct tcaatttgct gacggatgcg tgtcttaaaa
     2881 tctgtattac tggttttgat cgacatcttt atatcctcag cggctcatca gcacttcagc
     2941 aatatgcatc actttgactt tctgcccttc ccgttgtaat cgcccactga tgttcagcag
     3001 gcaactcacg tcagcaccta ttaaatactc agggcggact tccatcaggt gcgcaacctt
     3061 ttctttcacc atctcgccgg atatttcggc cattttgacc gagaacgtgc cgccaaatcc
     3121 gcagcaggta tcctgttcag caaaggtcaa caactccagt ccacgcacat ttttcagtaa
     3181 cgtaagtggc tcgtccttca cccccagctt acgggccagg ctacaagatg ggtgatacac
     3241 cgctctccct tgcaaactgg cacctacatc gactacccct aatttattaa caataaaaga
     3301 ggtgagatcc tgcatacgcg cggcaacctt ttcggcacgt gatgcccatt caggttcatc
     3361 cgccagatac gtcgggtaac tttttacggc ataggtgcaa gagccagccg gtgaaataat
     3421 gggatcgtcg ttatcctcca gtgcggcgat cagatttttc atccctggaa tcgcttcttt
     3481 gatataacca ctattgatcg caggctgacc gcagcatccc tgtttctccg ggaaatttac
     3541 gcgacagccg agtttttcca gtagcagcac ggagtctcgt gccattcttg atttcagggc
     3601 gtcaccaata caggtgacaa agaaattgac attcacaact aatactccat tacttcatgc
     3661 ccatttatgc ccgatggttc cccccaccgg gggatcaatc atcaataaat atggcgttct
     3721 tttttaattg gctagcaaca taccacccct catttgtatg acaaatgata atctttcatc
     3781 caaacttcaa atgcaataac taattttatt tctttcggca tcgctcacat tttatgggga
     3841 aatcgacgat catttttggc ataagaaatc catttaaaaa tggctacatc ccttatacct
     3901 attcagagct acttattcag gggttattga gatatacact gatggtgtac aaaaaaatca
     3961 ccgacaataa tcacgtatca agtaatagtc ggcattttat ttaattgaag caatttagtt
     4021 tgcattttta tttaaaaact gagctagagt ccatcagaaa aaaaatatat aaagcccaaa
     4081 ccaccgttgg aaatagaaat agttgaaagg ctcacaactc ccggaatgcg gattatcttt
     4141 gccctgattt ctggcggttt tattcagggt gcaagctgtc tgaccctttc ccgatattct
     4201 cccggagtac aaccaaactc gcggacaaac gccttgtgaa aagatgattc actggcatag
     4261 cctactgact cagcgatcac cacaacaggg agcatttccc gggaaaacat ctgggccgct
     4321 atttgtagac gcaactttgt taatacagct agcggcgtgg ttccggaaac atcacggaaa
     4381 agctgggcaa aacttgcccg ggacatgtgg gcgatgctgg ccagcgattc gacggtccag
     4441 gcgtgtcctg gcatttccag catttgctgt attaccgcac caagacgtgg atgcagaagc
     4501 aaactgagaa tgtttttctc tgtattaacc tgtgcaatcc actcacgcac cgcgagggta
     4561 aagaatgtag cgcagatctg gctacacaga gcatccactc ccggcattgc cgatctggat
     4621 tcctgttgta aaaacgggat cgcctccctc agccagttat attccacgct gtggttaacc
     4681 ggtgctacaa acagcgtttc cggcaaagac gttaaaaaat aacgcgccga atgttgcagc
     4741 cgaagagtgc cacagacaat acaggtcgat tcattatcga catgactcag gcgatgagct
     4801 gaattttgtg gtagcagaac cacatttccc gggcgtaatg taaaaatctc ccccgtcggc
     4861 atttccagct tcgccgctcc ttgcgttaac gcatgccaac gaataaccga taattccccg
     4921 gcaccatgtg gaagctgcca gtcacttcct aacacgcaat tcttatcgat cgttccttgt
     4981 ggagcgttaa gcatcaacaa acggctaagg gcatccatat cagactcctg agtggttttt
     5041 gattacaaac accaacaatc ttagtaaata actaatctaa taccatcaaa tagttaatca
     5101 tgatttttgt tttgtgattc tgattaagaa aaagaaaata ccagacgata agacaaaata
     5161 tcaagcgttg tgaagaaatg gtatttgctc ttttgcgtct atagtcatga tgtcaaatga
     5221 acgcgtttcg acaggaaatc atcatgaata aatatcaggc agtgattatt ggttttggca
     5281 aggctggaaa aacattagcc gtcacgctgg caaaagcagg ttggcgagtg gctctcatcg
     5341 aacaatcaaa tgcaatgtat ggcgggacct gtattaatat cggctgcatc ccaaccaaaa
     5401 cattggttca tgacgcacag cagcacacag attttgtccg tgccatacag cgtaaaaatg
     5461 aagtggttaa ttttttacgt aataagaatt ttcataatct tgcggatata cccaatatcg
     5521 acgtgatcga cggccaggcg gagtttatca ataatcatag cctgcgtgtt catcggcctg
     5581 agggaaatct ggagattcat ggcgagaaaa tttttattaa taccggtgca caaaccgtgg
     5641 ttccgccaat tcctggaatt accaccacgc caggtgtata tgacagcacc ggattactta
     5701 atctaaaaga attgcctgca catttaggta ttttgggcgg cggatatatt ggcgttgagt
     5761 tcgcctctat gttcgctaat tttggcagca aggtaaccat tttagaagca gcttcgctgt
     5821 ttttgcctcg ggaagatcgg gatattgctg ataacatcgc gacgatttta cgcgatcagg
     5881 gcgtcgatat tatcctcaat gcccatgtgg agcgaatcag tcaccatgaa aatcaagtgc
     5941 aagtgcatag cgagcacgcc caactggcgg tggatgcact gttaatagct tccggtcgtc
     6001 aaccggctac cgcttcgtta catccagaaa atgcaggtat cgcagtaaac gagcgcgggg
     6061 caattgtcgt tgacaagcga ttacatacca ccgcagacaa tatttgggcg atgggagatg
     6121 ttaccggcgg gctgcaattt acttacatat cactagatga ttaccgcatt gtacgtgatg
     6181 agttactggg tgaaggcaaa cgtagtactg atgatcggaa aaatgtgcct tattccgtat
     6241 ttatgacacc gcccctgtcc agggttggta tgacagaaga acaagccaga gagagtggtg
     6301 ctgatattca ggtggtgaca ttgcctgtag ctgcaattcc gcgcgccaga gtgatgaacg
     6361 atacccgtgg ggtattaaaa gcgattgttg ataataaaac ccaacgtata ttaggggcat
     6421 cactgctgtg tgttgactcc cacgagatga tcaatatagt gaaaatggtg atggatgccg
     6481 ggctgcctta tagcatatta cgcgatcaga tatttactca tccgtcgatg agcgaatcac
     6541 tcaatgatct attttcatta gtcaaataaa ctcaaaatca gacgccagaa caaatattct
     6601 ggcgtctcag agaaaagaat cttattaatt ccctgttact ttataacttc ttaatcactt
     6661 cattgatggt attttatatg tttaaaaaat ctgttttatt tgcaacacta ttatctggcg
     6721 ttatggcatt ttccaccaat gcagatgata aaataattct gaaacatatc agcgtctcat
     6781 cagtatcagc atcaccgaca gttcttgaga atgccattgc tgatatggcc agaaaatata
     6841 atgcttcatc ctggaaagtt acatcgatgc gaattgataa taattcaacc gcaacagcag
     6901 tattgtataa ataaggatgt ttacaatgga aagatacctg cacctgttaa gtcggggaga
     6961 taaaattggc ctgacattga ttcgtctgag tattgcgatt gtttttatgt ggattgggtt
     7021 attaaagttt gtcccttacg aggcagacag cattacacca ttcgtcgcaa acagtccact
     7081 aatgtcgttc ttttatgaac acccggaaga ctataaacag catctgactc acgaaggcga
     7141 atacaaacca gaagcaagag catggcaaac ggctaataat acctatggtt tttccaacgg
     7201 tcttggcgtc gtggaggtga ttattgctct gctggttttg gctaatcctg tcaatcgctg
     7261 gttaggttta ttgggtgggc tgatggcatt taccacgccg ctggtaacac tctcattttt
     7321 aatcaccacc ccggaggcat gggtacccgc attgggtgac gctcatcatg gtttccctta
     7381 tttatccggt gctggtcgtc tggtattgaa agatactctg atgctggcag gtgctgtcat
     7441 gataatggca gattcggcgc gggatattct taaacaacgc agtaatgaat ccagttcaac
     7501 gttaaaaact gaatattgat aatccgaaca ctgtctgttg ttcacttttt caggcgtcag
     7561 gccccctttc tgatgatgaa gcctgacgcc atattaccgg agatatgaca gagtgaagat
     7621 caatatcact cccctgttaa aagattcttg tcgtctcagg taaaatatat gattaatatc
     7681 aatcatgtat ttttacagca agacaaccac gaacaaattc aggattaaaa tgttttaccg
     7741 actccccaac ataacccaaa tcaagcgaac taatttgtgc catttctttc tctgtcagtg
     7801 agaaatccca gatagcaaaa ttttcttcga tacgctgttg atgtgtcgat ttcggaataa
     7861 cgaccactcc ccgttgaata ttccaacgca gaatgacttg agaaatcgat ttttgatggg
     7921 catctgcaat actctgaagc agattatttt caaagggttt atgtcgtcca ccacctaacg
     7981 gagcccatgc ttcaggctgc acgttataat acttcatggt tgccagcgcc tctggttggg
     8041 caaaataggg atgcaactcg acctggttga ccatcggttt aactcttacc gtttcacaaa
     8101 agttcgccag aacatgagga tagaaattgg aaaccccaat tgcttttaat ttgccctctt
     8161 cataggcatc ttcgagtgca cgccacgcac tgaaataatc gcgcatagcc tggtgcaata
     8221 aatagaggtc gaagtactct agtcccgatt tttttaatga tgcttcaata cctgctgctg
     8281 ccgtatcctg attcaacata tcctgcaccc acaatttaga cgttataaat aactcatctc
     8341 gggtacataa accttcagat atagcttcac gaacggcttc accgacggca tcttcattac
     8401 cgtataccgc tgcagtgtcg atgagtcgat aacccgtgcg gatagcgttc aacactgact
     8461 gtttgcacac gtttttatcg gtaacctgaa aaacaccaaa tcccatcatc ggcattttca
     8521 gattgttact taatacggaa aattccactg tcagtccctc cattaggcat gaacaatgag
     8581 tctacgttaa aacgtaacct caaattagta tgtggatttt gatatcactt atgcaaaaaa
     8641 ttcattaata atgtaggact gaaacctctc tattttcggg gacaacgaag cagacgctac
     8701 cagtgctttt gccttcggcc ttgctgtttt tgatacactt agggcccagg gcataacgaa
     8761 aatgtgcgat atgacgaaga attaatggag aatgtgatga tcaggcagaa gattctacag
     8821 cagctcctgg agtggattga gtgcaatctc gaacacccta tttcaatcga agatatcgca
     8881 cagaaatctg gctacagcag acgcaacatc cagcttctgt tccgaaattt catgcatgtg
     8941 cctttgggag aatacattcg caaacgaagg ctttgtcgtg ccgccattct tgtccggctc
     9001 accgcgaaat ctatgcttga tattgcactc tctttgcatt ttgactcaca gcagtcattc
     9061 agccgcgaat ttaaaaagtt attcggctgc tctccccgtg aataccgcca ccgtgattat
     9121 tgggatctcg caaatatctt cccttctttt ttaatacgtc aacagcaaaa gacggagtgt
     9181 agattagtca actttcctga gacacctatt tttggtaatt catttaaata tgatattgaa
     9241 gtgtcgaata aatcaccgga tgaagaagtc aaactgcgac gtcatcattt agccagatgt
     9301 atgaagagtt ttaagacgga tatctatttc gtctccacgt tcgaaccatc aacaaaatcg
     9361 gtcgatttgc tcacagttga aacttttgcc ggtacggtat gcaaacaaac cgattcagag
     9421 atgtcgaaag agtggacaat taaccgagga ctgtatgcct ctttccgcta tgaaggtgag
     9481 tgggagcact accctgaatg ggcgcgaaac ctctatctga tggaattacc tgccaggggg
     9541 ttagccagag tgaacggcag tgatattgag cgattttatt acaatgaaaa cttcgtcgaa
     9601 attgatagca ataacattgt ttgcgaaatt tttatccccg ttcgcccggt ttagcctgta
     9661 actccatcac ataagattta ccctaccctg ccaccgtcta atcggtgcca gggtaggtat
     9721 tcagtaaata atggcgctgc gcatgtcaac gccattatta tttattacat cgtcgttttt
     9781 atttctcctc agcgccttca gtatctgcag gaacggcgtt aattgtcaca gtcactggtt
     9841 tcatttcata ccctccatat gtcaaagagt tgaatgtgaa tgtgtatgta cctggcttgt
     9901 tggtactaaa tgtacgcgtg attttcccgc tcgcgaaatg gtcatttttc gacggcagga
     9961 actgataatc cgcctctgtc accccttccg gagcctcaat gtcgacccac atggttcctc
    10021 ctaccgggtt gtttgccgag aacagggtta ccgtcagtgt tgctgtcgtt accccatccg
    10081 ccaccagaga atgcacatca gatgttgcgg cgagctccgc gtttttatca tccgcgacga
    10141 atttagattc ggcagttttc gtcgattcct gaccgtcggc ctgttccact ttcgccgtga
    10201 gtgtatatgt cgctgcgaca gtggtcgtgg cggtgaaaat agcctgccct tgctcattcg
    10261 ttgtcgccgt cccattggga gtcagaacta aattttccgg gctggcagtc agcgtcactt
    10321 cgctatcttt cagcaggttg ttattggcat cagtcaccgt aactttatac gtctgtttgg
    10381 ttttgccgtc cgccactgca cggttgccaa tcacctcaat actggagatg gtcgccgtag
    10441 tgttatcggc cacaaacgtt acagactgcg cattcgaagg ctggtcattt cctgctgacg
    10501 ccgtgatctc ataggttccg gcagtcacgg aaaccacttt cagttccgct ttaccctcct
    10561 tgtcggcgtt caccatgata ttaccgtctg caagcggttt gacgccccga ggcagattaa
    10621 agacgaccac agcgccagga agaaggttgc cgtattgatc cttaaccgtt gccgtcagcg
    10681 taaaggcatc tttgccgttt gccacttttt gagcagcggc gtctacctcc aggctcgcct
    10741 gaccggtact gaaatccgcc ttgaatttca ccttgaccgt cttctgagag tttttcaccg
    10801 aggcctcgat ctcaagttcc cctgcaaccg ttgacataag ctcaatgtcc actttccctg
    10861 ccgcattggt tgttactgta ttccccgtct tgctggtcac accctgcggt aaagtcagcg
    10921 taacttcttg cccctgcaac gggttaccat agctgtccac gacggtcagg gttatctggt
    10981 tagcagactg tccattagcc agttggttat taaccttcac tgtcatatca cgaatctcag
    11041 ccttagatgc gtcacctgca acattcagca ccagtggctg agcaacggca ttttggccgt
    11101 tcactcgcgg catcacagac aactgacccg cggcagatcc cagcgttaag gtcgacacgt
    11161 agaccccatt acctttctct gtccagtttc ctgctgaagg acgctcactc cccgtgctgg
    11221 cggcaccgct aaacactggt gcatctggct tcagcccggt gaccgtgttc ccgtacgcat
    11281 ccttcacggt gaaggtgagt tccgtcgtcg ttttgacggt cggagcctta ttgtccgcaa
    11341 caagcgtaga gttcgctgat gacatctctc cggcgatgac cgtcaactgc gcggcttcgg
    11401 tggctgctgg ctggccgttg aatagcggca tgacgagaag ctcacccgtc tttccacctg
    11461 ttgtcagcgt agcgacatag gaaccatcac ctttttcggt ccaacaagaa acagtcgccc
    11521 cttcagacgc agcacccgtc aggcttgccg acaacgcaag accactgata gcgttgccat
    11581 gcgcatcttt cgccaccagc gtcacggttg tgctttcgcc ggcttttacg tgatcttctg
    11641 cgacagagac tttcgactgg tttgaagata acgcatcagc caccacggtc actttagccg
    11701 cattcgctgc cgcatcctgt ccatttagct tcggcataac ctctaattca cccgccgtag
    11761 tgccgagaga aatctgcgca gtccaggtcc cgtcgccatt atccgtccag cctgatgccg
    11821 tagaaccagc agcagctgcg cccgataatg acggtgcatc cggattgaga ccagttacag
    11881 ggttgtcatt agcatctttt gccgtccaga ttgccgtaac tgtaccgcca accaccggtt
    11941 tatcaggatt cagtgtgatg gacgaatgtg ctgcatcaag cggcccggca acaaacttca
    12001 gcgtttgttg taatgtgccc agctgatact gctcgctgaa ggcacgcaca acaacgtttc
    12061 cggcacgggt cgaagaaacc gtggcgctgt aaacccctgg ttttatttcc gaaatggcac
    12121 caacggttac accattagtg tcttgcggaa caagtcgcag gcggctggct tctcccgtca
    12181 ccggattacc ctcggagtcc accgctgtca gtgtcagcgt gtaggcttgc tgaccatcag
    12241 caaccacatc acctgacggc tcgttagcgc tcagggtgga gtttgccaca tccatcatcg
    12301 tggcccgcag ttctgcagtg acagttttgc tcatgtcatc aacgctaaca gtaatcgttg
    12361 cctcacctga ctgcgttccg gtagtaaaga cagactgata cacccctgct tcagtttcgg
    12421 tgaactcacc cagtgttggc tttgcctgtg atttagtggc cttcagggta cgagtcacaa
    12481 tatttccagc cggtttgaag gttagttcag tcttgatctg atctttcatg cccgtgactg
    12541 gctgcccctc ggcgtcgcgc agagacagca ccaacggctt ttgctcatta ccgttagcaa
    12601 gcatttgaat acggctctga ccgtcaagcg ttaacgccgt acgatcggcg ctcataccag
    12661 ctccgctaat aaccacttct gtctgcacac gtttcgaggc attgcctttg ttatcgtagg
    12721 caatcgctga aatcgcataa taattgtctt tgcctgcctg ataagccggg agcgtcactt
    12781 gccactgatt gccctgcccc gtaatttttc cgcctgcggc cagcaaagac ggcgcttccc
    12841 attgcacatt tttcagaccg tgagttgctt tgctgaccac aagccccagg gaaaccgtct
    12901 ggccgccctt gccttcaata cgctcaggca gagcaatacg gatcacttca gatttgcgat
    12961 actcaagaac gatattgtta ttacgctcaa ccaggtcata gcggctgcct gccagcattc
    13021 gacgctcgcg aatgctgtct gtatcgagtt gtttttccag aggttcgcca atccgataat
    13081 taacttccag gccaaagcga gtgtcattct caccactctt gccctgctta tgcccggcac
    13141 tcagggtcag aagaggcact ggcgtgtaat tcacttcagc ggtaatcgca tgtggatctt
    13201 tctggcgttt atctttacca aacagcccga cttcatcgcc ataatactgt tcatacatca
    13261 ggcttgcgcc aagctgcggc caggcaggta aatagccctc agcacgaata tcccagccat
    13321 tcgccgggcg ttcctgataa tcctcaacat ccggcgatgt tttccagcca gaagcccgga
    13381 tataaccatt ggcgctcagt ttcaaataat cacgccagta ttccgcacca acaccaatgc
    13441 gggtatgact acgggataaa tcatgatcga taaaagtatt cacccccgcc atccagtcat
    13501 tttctgaaaa atgacgccag ccaaaaccaa tatttgactg agtacgatcg tcggtacgat
    13561 gtattgcccc ctgagtgaac aacatatttg tcggtgtatc ataaatcgga taaagcattt
    13621 ccagcgaaga gtccttcagc gagaaatttt tatcgacatt cagtttgacg cgcgcagtac
    13681 cgtatttccc gagccactcc tgaatttcct ggttagcttt agcggtggcc attccggtaa
    13741 taaagttacg tgtcgcatcg ctatctggct gactgcttaa aaatgtcccg gcatttgcgg
    13801 caaacgacgc gacatttttc tccacgttat tatcagcagt taccgtagta tttcccatgc
    13861 tcaaccgtgg ctgaaccgca tgctgcgcac gtgccgccat tactggggta aaggtgacag
    13921 cgagtggaaa aagaacctga acagagatat ttgtccacgc cacgcagcgg gccagaactg
    13981 aataacgaaa tcgtggttgt ttatgacctg ttttataacg tgacattgag ataatttcct
    14041 gatgaacgaa ttaacgaaca taggcagaca caaataatgg cgtctgcctg tcagaaaaac
    14101 cgggaaatcc ccgatttcct tttccgcaga aaaggaaata cctttatgta agaaagtaca
    14161 ccgccaccta atatatccag tgtctgtagc cactcatcag gtggttgggc gcgggattat
    14221 atttttattt atgggtaaaa atttagctt
//
LOCUS       DAPHCV010000114        14186 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.114, whole genome shotgun sequence.
ACCESSION   DAPHCV010000114 DAPHCV010000000
VERSION     DAPHCV010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.114"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            28..594
                     /locus_tag="RH033_003739"
     CDS             28..594
                     /locus_tag="RH033_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV2992863.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSNNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            645..3104
                     /locus_tag="RH033_003740"
     CDS             645..3104
                     /locus_tag="RH033_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV2992864.1"
                     /translation="MDTVNIYRLSFISCLVMAIPSALAVEFNLNVLDKSMRDRIDISL
                     LKEKGGIAPGEYFVSVTVNNNQISNGQKIDWKKNGDQTIPCINDLLVDKFGLKPEVRQ
                     SLPRLNQCVDFSSRPEILFIFDQASQQLNITIPQAWLAWHSDNWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSNSTNLNAYGTAGINAGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTVSHSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRANNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRTSMSHQTENETFFSNEVSWGMLSNTSLYGGLLISGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSQFDTQQDERGLSYRFNYSKQVDATDSTISLA
                     AYRFSDRHFHSYANYLDHKYNNSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGNWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSTGLQSDRPDNGVQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDNSGISVGMVGEEGHAWLSGVAENQMLTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3119..3850
                     /locus_tag="RH033_003741"
     CDS             3119..3850
                     /locus_tag="RH033_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDV2992865.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPSPEKNSGHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVITNTGSYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCRLQAVEAKK"
     gene            3847..4908
                     /locus_tag="RH033_003742"
     CDS             3847..4908
                     /locus_tag="RH033_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2992866.1"
                     /translation="MSAGKGVLLVICLVFLPLKSALALNCYFGTSGGTVEKSEAIQPF
                     AVPGNAKPGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDASLGKSRIDTNQCIDSKNINIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLIEEDKALLIGNGLKLRLLDENSSPYTFNKYSEYADFTSDMLIYEKTYTAELSSIPG
                     TPIEAGPFDTVVLFKINYN"
     gene            5061..6107
                     /locus_tag="RH033_003743"
     CDS             5061..6107
                     /locus_tag="RH033_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDV2992867.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLGLHLPAALLLGPMLAGIIFSMRG
                     VTLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6104..6895)
                     /gene="nei"
                     /locus_tag="RH033_003744"
     CDS             complement(6104..6895)
                     /gene="nei"
                     /locus_tag="RH033_003744"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HDV2992868.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKPYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTMRVLRVKLQTVDKTIL
                     LYSASDIEMLTPEQLITHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6931..7665)
                     /gene="pxpA"
                     /locus_tag="RH033_003745"
     CDS             complement(6931..7665)
                     /gene="pxpA"
                     /locus_tag="RH033_003745"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HDV2992869.1"
                     /translation="MKIDLNADLGEGYASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSVMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKGIVVAA"
     gene            complement(7655..8587)
                     /gene="pxpC"
                     /locus_tag="RH033_003746"
     CDS             complement(7655..8587)
                     /gene="pxpC"
                     /locus_tag="RH033_003746"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HDV2992870.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8581..9237)
                     /gene="pxpB"
                     /locus_tag="RH033_003747"
     CDS             complement(8581..9237)
                     /gene="pxpB"
                     /locus_tag="RH033_003747"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDV2992871.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9260..10003)
                     /gene="ybgI"
                     /locus_tag="RH033_003748"
     CDS             complement(9260..10003)
                     /gene="ybgI"
                     /locus_tag="RH033_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HDV2992872.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10274..11755
                     /gene="dtpD"
                     /locus_tag="RH033_003749"
     CDS             10274..11755
                     /gene="dtpD"
                     /locus_tag="RH033_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HDV2992873.1"
                     /translation="MNKYASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGVVLMIWLYQALKFRNRALALES"
     gene            complement(11798..13216)
                     /gene="phrB"
                     /locus_tag="RH033_003750"
     CDS             complement(11798..13216)
                     /gene="phrB"
                     /locus_tag="RH033_003750"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HDV2992874.1"
                     /translation="MTTHLVWFRQDLRQHDNLALAAACRNSSASVLALYIATPRQWAA
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFAASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVQVERTLRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFQNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRDVPGKVVHEPWKWAQKAGMTLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13213..13722)
                     /locus_tag="RH033_003751"
     CDS             complement(13213..13722)
                     /locus_tag="RH033_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HDV2992875.1"
                     /translation="MNQQRFDESTLIRIFAIYELYRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHRWQSLDDYYNQYRQRVIVLLSHPANRRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSVQRYFELWPCV
                     INLRHSGVL"
     gene            complement(13873..14186)
                     /locus_tag="RH033_003752"
                     /pseudo
     CDS             complement(13873..14186)
                     /locus_tag="RH033_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         3419 a         3780 c         3466 g         3521 t
ORIGIN      
        1 ctatctcatt atttaaagga ctcagctatg tttaaaggac aaaaaacatt ggccgcactg
       61 gccgtatctc tgctgttcac tgcacctgtt tatgcggctg atgaaggatc cggtgaaatt
      121 cactttaaag gtgaagttat tgaagcaccg tgtgaaatac atcaggatga tattgataaa
      181 gaagttgaac tcggtcaggt gaccaccagc cacattaatc agtcgcatca cagcgatgcc
      241 gttgctgtcg acctgcgctt agtcaactgc gatctggaaa actccaacaa cggttccggc
      301 ggcaagattt ctaaagttgc agtcaccttt gacagctcag cgaaaaccac cggcgcagat
      361 ccgattctca acaacaccag cacgggtgaa gcgaccggtg tcggcgtgcg tttaatgaat
      421 aaagatcaaa gcaatatcgt tttaggcacc gctactccag atatcgacct agcaccaacc
      481 tccagcgaac aaacgttaaa tttctttgcg tggatggaac aaattgatca ggctacgccc
      541 gtaacaccag gcgcagttac ggcgaatgcg acctacgtac tggattataa ataagtatca
      601 ctttggcggc gtcatcgccg cctttcttca aatatttatc aggtatggac accgtgaata
      661 tttatcgact ctcttttata tcctgcctgg tcatggcgat accttccgca ttggcggtcg
      721 aattcaacct gaatgtcctc gataaatcga tgcgagatcg cattgatatt tcattattaa
      781 aggaaaaagg gggcattgct cccggtgaat attttgttag cgttacagta aataacaacc
      841 aaatcagtaa cggacaaaaa attgactgga aaaagaacgg tgaccaaacc attccgtgta
      901 ttaatgattt actggtagat aaatttggtt taaaacctga agttcgtcag tcgttaccac
      961 ggttaaacca gtgcgtcgat tttagctccc ggcctgaaat tctcttcatt tttgatcagg
     1021 ctagccagca actgaatatc actattccac aagcctggct ggcgtggcac tcagataact
     1081 ggactccccc ctcaacatgg aaagaaggtg tcgccggcgt cctgatggac tacaacctgt
     1141 ttgcaagcag ctaccgccca caggatggta gcaacagcac taacctgaac gcctatggaa
     1201 cagccggaat taacgcggga gcatggcgtt tacgcagtga ttaccaattg aatcagaccg
     1261 atagcgatga taaccatgaa cagtcaggcg aaatatcacg cacctatctt tttcgtccat
     1321 taccgcaatt aggttcaaaa ttaacccttg gtgaaacgga tttcagttcc aatattttcg
     1381 acggcttttc ttataccggc gcggcactgg caagtgacga tcggatgtta ccgtgggagc
     1441 tacgcggcta cgccccacaa atcagcggca ttgcacaaac caacgccacc gtcaccgtga
     1501 gtcactcagg gcgagttatt taccagaaga aagtcccacc aggtccgttt atcattgatg
     1561 accttaatca gtccgtccag ggtacgttag atgtcaaagt gacggaagaa gatggtcggg
     1621 cgaacaattt ccaggtttcg gcagcatcga cgcctttcct tacgcgccag gggcaggttc
     1681 gctataaact tgctgccggt cagccgcgaa catctatgtc tcatcaaact gaaaatgaaa
     1741 ccttttttag caatgaagtt tcctggggga tgctgtcaaa tacctcactg tacggcggcc
     1801 tgctgatttc gggtgacgac taccattctg ccgcaatggg tatcgggcaa aatatgctgt
     1861 ggcttggtgc attgtcgttt gatgtcacgt gggccagtag tcagtttgat actcagcagg
     1921 acgagcgggg cttaagttac cgctttaatt acagcaaaca agtggatgcc accgacagca
     1981 cgatttcgct cgccgcttat cgtttctccg atcgtcattt tcacagctac gccaactatc
     2041 tggatcacaa atacaacaac agcgatgcgc aggacgaaaa acagacgatc agcttatccg
     2101 tgggccaacc gattacccca ctgaacctca atctgtacgc caacctgctg catcaaacct
     2161 ggtggaatgc agacgcctcc acgaccgcca acatcacggc gggttttaat gttgatattg
     2221 gtaactggag agatatctcg atctcgacgt cattcaatac aacccattac gaagataaag
     2281 atcgcgacaa tcagatttac ctgtcgattt cgctcccctt cggtaacggg gggcgggtcg
     2341 gttatgacat gcaaaacagt agtcacagca ccacacaccg catgtcgtgg aatgatacgt
     2401 tagatgaacg taatagctgg ggcatgtcta ccggactgca atccgaccgt ccggataatg
     2461 gagtccaggt gagcggtaac tatcagcacc tgagttcagc gggtgagtgg gatatttctg
     2521 gtacctatgc cgccaatgat tacagttccg tcagcagcag ctggagcggt tctttcacgg
     2581 caactcaata tggtgcggcg ttccatcgtc gcagctccac caatgaacca cgtctgatgg
     2641 tcagtaccga tggcgtggca gatattccgg ttcagggcaa tctcgactac accaaccatt
     2701 ttggcattgc ggtggtgccg ttgatttcca gttatcagcc ttccaccgtg gcggtgaaca
     2761 tgaatgactt acccgacggc gtaacagtgg cagaaaacgt tatcaaagaa acgtggattg
     2821 aaggcgcgat aggttacaaa tcgctggctt cccgttccgg taaagacgtt aacgtcatca
     2881 ttcgcaacgc cagcggtcag ttccctcccc tcggtgcgga tatccgccag gataacagcg
     2941 gcattagcgt ggggatggtt ggcgaggaag gacatgcctg gttaagcgga gtcgctgaaa
     3001 atcaaatgct taccgtggtc tggggtgata gtcagcattg ctcgctccat cttcctgaac
     3061 atatggaaga caccgcaaat cgactgattt taccttgtca ttaattaagg aaatgaaaat
     3121 gacatttatg aaaggactac cccttttgtt gttggttgcc agtctgtgca gccacgctgc
     3181 actacaaccc gatcgcactc gtattgtgtt taacgctaat gataaagcta ccagtctgcg
     3241 cgtggataat cgtagcgata aactccccta cctcgcttat tcctggctcg aaaatgaaaa
     3301 aggggagaaa agcgatgatc ttctggttgc cctgccaccg atacaacgtc tggaaccgaa
     3361 agcaacaacg caggtacgga tagtaaagca agcctcgacc acaaaattac cgggcgatcg
     3421 cgaaacgcta tttttctaca atatgcgtga aattcctccc tccccagaaa aaaacagcgg
     3481 ccatgccgtt ctgcaagtcg ccattcagag tcgtatcaag gtattctggc gaccggctgc
     3541 attacgcaag aaagcaggag aaaaggtcga gctacagtta caagtcagcc agcagggcaa
     3601 ccaactgaca ctgaaaaatc ctaccgcgta ttatctgacc attgcctatc tggggagaaa
     3661 tgaaaaaggc gttcttcccg gcttcaaaac cgtgatggtt gcgcctttta gtacagtaat
     3721 tacaaacacc ggaagttata gcggcagtca gttttatctc ggttatatgg atgattacgg
     3781 cgcgttacgc atgacgacgc tcaactgcag cggacaatgc cgtttacaag cagtggaggc
     3841 gaagaaatga gtgctggcaa gggagtgttg ctcgtcatct gtctggtatt tctgccattg
     3901 aagtctgcgc tagcgctgaa ctgctatttt ggtacttccg gcggaacggt agaaaaatcg
     3961 gaagcgattc aaccctttgc tgtgccaggt aatgccaaac ccggcgataa gatctgggaa
     4021 tctgacgata ttaaaattcc cgtctattgt gacaacaata ccaacggcaa ctttgaaagc
     4081 gaacatgtct acgcatgggt aaacccctac cccggggtac aggatcgtta ttatcaactc
     4141 ggcgtgacat acaatggtgt cgattacgat gccagtctgg ggaaaagccg catcgacacc
     4201 aaccagtgta tcgacagtaa gaacattaac atttataccc ctgagcagat tatcgcgatg
     4261 ggctggcaga acaaaatatg ctcgggtgac cccgctaata ttcacatgtc gcgaaccttt
     4321 ctcgctcgta tgcggttata cgttaagatt cgcgaaatgc cgccgcatga ttatcaaagt
     4381 acgcttagcg actatatcgt cgtgcaattt gacggtgccg gtagcgttaa cgaagaccct
     4441 actgcccaaa acctgaaata tcatattact ggtctggaaa acattcgcgt gctggattgc
     4501 agcgtcaatt tttccatttc accggaaaca caggtgattg attttggtaa atttaatttg
     4561 ctggatattc gccgccacac aatgtcgaaa acattcagta ttaagacgac aaaatcacaa
     4621 aatgaccagt gcaccgacgg atttaaggtc agctcttcgt tttataccga agaaacattg
     4681 attgaagagg ataaagcact gctgattggt aatggactga agctacgttt gctggatgaa
     4741 aattcttcac cctatacttt caacaaatac agcgaatatg ctgatttcac cagcgatatg
     4801 ctgatctacg aaaaaaccta tacggctgaa ctttcgtcca ttcccggcac ccccatcgaa
     4861 gcgggtccct tcgatacagt ggtgcttttt aagattaact ataactaatt tatcatgatg
     4921 ccctttgcag tcagtaaagg gcctctcttt cagaaaattg ctgattttcc ccttttcctt
     4981 gattgctgtt attttttcct ggcccggctt tctttatgct gtatcgatac accaaagaga
     5041 aaataatgag ggagcgaagg atgccagttt tgcagtgggg aatgttatgt gtgctctcac
     5101 ttctactttc tattggcttc ctcggattac atctcccggc ggcgctatta cttgggccga
     5161 tgctcgccgg gatcatcttc agtatgcgcg gggttaccct gcaactcccc cgctccgctt
     5221 ttctcgccgc gcaggccatt cttggctgca tgattgcgca aaacctgacc ggttcgatcc
     5281 tgaccacgct agcggtgaac tggccgattg tgttagctat tttactggtg acgttacttt
     5341 ccagcgccat cgtgggttgg ttattggtgc gctatagctc gctgccagga aataccgggg
     5401 cctggggttc ttctcctggc ggcgcggcgg caatggtcgc aatggcgcaa gattacggcg
     5461 cagatattcg cctggtggcg tttatgcaat atctgcgagt gctgttcgtc gcaggagccg
     5521 ctgttctggt cacccgcatg atgctgggcg ataacgctga agcggttaac cagcagatcg
     5581 tctggttccc gccagtgagc attaatctcc tgcttacgat tttgctcgcg gtcgttgccg
     5641 gtacggcggg atgcttgcta cgtcttcctt ccggcacgat gctcatcccg atgctggcgg
     5701 gcgcagtgct ccagtctggt cagctcatca cgattgaact cccggaatgg ctgctggcga
     5761 tggcatatat ggcaattggc tggcggattg gtcttggttt cgataagcaa atattactgc
     5821 gggcattgcg cccactgcca caaatcctgc tgtcgatttt tgctctgctg gctatttgtg
     5881 cgggtatggc gtgggggctg acccggttta tgcatattga ttttatgacc gcctacctcg
     5941 ccaccagccc cggcgggctt gataccgtgg cggtcatcgc cgcagggagc aatgccgata
     6001 tggcgctcat catggcgatg caaaccctgc gcctgttcag tattttgctg acgggacctg
     6061 ccattgcgcg gtttatttca acctatgcgc cgaagcgttc tgcctaatgc tggcagccag
     6121 ggcaccagta aaacgggcga gatgacagcg tggttttctc aatgatgccg ccacagcgtt
     6181 cgcacagttc gccatcacga tgaaaaacct taaagcgaaa cagcgcccca tgatgcttat
     6241 tctcatccac ctgcccccgc gtagcgtagg aaaatcgagg aatatccagt aacgcgtgtg
     6301 cgagtgcatc cagttgcgcc gcattgagat ctttcgcttt atgatttcca gtcaacccaa
     6361 cctgccagag gatctccacc cgcaaataat tgccaagccc agccagaaac gcctgatcga
     6421 gcagtaatcc agcaaactga cggttacgaa agcgcggcga caataaccgt tcctttacca
     6481 cctccggcgt cagattcgga tccagcacat cggggccgac tcgttgtaaa aacggatgcg
     6541 tgatcagttg ttccggcgtc aacatctcaa tatccgaggc gctataaagc agaatagttt
     6601 tgtcaaccgt ttgcagtttt acccgcaata ctcgcatagt ctgcggctct tcgccggtat
     6661 caaccacgcg ccagacgccg taaagctgat tatggctgta gagcgttaag tcgttggaaa
     6721 aatgagttaa caacgcctta ccacgcgttt ccacatgggt aacgtgttga ccgataagtt
     6781 gtgattgata aggttttaac tgcgggaagg caaaccagac atcagttagt ggtttgcctt
     6841 tgatcgccgc ctccaggtta tccgctgcac ggcggatctc cgggccttca ggcatggtga
     6901 tatccttttt tcattcttta ttaaacgggg ttatgctgcg acaacaatcc ccttttcggc
     6961 aaatgtagag cgtagtcggc gggcgaatgc cagtgcgtgc tcgccgtcgc catgcaggca
     7021 gaccgtttgc gccgtgaccg ttgcccattc gccggtgatg cttttgactc tgccgtgttg
     7081 caccatctcc agcgtttgcg ccagcgcctg ttcttcgttt tcaatcaacg cgcctggctg
     7141 gcttcgcggc accagcgaac cgtctgcctg ataaccgcga tcggcaaaca cttcctcgcg
     7201 cgttgtcagg ccatattgct tgcctgcacg aatcagctcg cttcctgcca gcccgacgag
     7261 aatcaatgcc ggatcgcaag cgtataccgc tctggcgatg gcgtctgcca gttgtgcctc
     7321 tttcgctgcc tggttgtata acatgccgtg aggtttgaca tgacgcataa caccgccttg
     7381 cgcacgggca atcgttgcca gcgcgccaat ttgatacagc gtctgggcgt aaacggtttc
     7441 tggcggcagc tgcatgacgc tgcgcccaaa attttccctg tcgggaaaac tcgggtgagc
     7501 gccaatcgca acaccatttt ttattgcttc acgcacgcaa gcctgcatgg tttgcgcatc
     7561 gcctgcatga aatccacagg caatattggc agaggaaacc agcgttaata gctctgcgtc
     7621 gctggcatag ccttcgccca gatcggcgtt caggtcaatt ttcattgtgc agccgccacg
     7681 ctaattgttc gaaataacgt tgctgatctt gccgtgcttt cagtgcttct tccagtgaac
     7741 actggacaaa atgaatcggc tgaccgagcg gaatttgcgc cagatggtac atatcagcct
     7801 cgatgataca ggcaatacgc ggataaccac cggtggtctg tgcgtcgttc atcaacacaa
     7861 tcggctgccc gttatgtggc acctgcacca cgcccggtaa caaaccgtga gataacagtt
     7921 cgcgatcggt ggtgcgtttt aaaatttgcc cctgtaagcg atagcccatt cggttacttt
     7981 gcgagctaag ctgccagggc gaacgccaga atgcatcctg cgaggcgcga tcgaactcat
     8041 gatattccgg ccccggcaag gcgcgaatgc ggttgcccca cagcagctgt ttaacgccct
     8101 gcgcttccat aaaatcacgc ttcgatttgc caatcgggag tcggtcacca tcctttagta
     8161 aacggccttc cagcccgcca atccccactt tgagatcggt gctgcatgac cccattaccg
     8221 gcggaacatc aataccaccc gcgaccgcca gataactgcg catcccgtgc tgcgggcgtt
     8281 taagcgttaa acgctgacct gctttcatcg gcaatcgcca gccggtccag acggcgttat
     8341 catccagccg cgcttcgcaa ccggcccccg tcagagcaaa ccacccatca gtttcgaact
     8401 caaccgttaa ctgaccgagc gtgatctcca gcgcgggggc attggcgtca ttacccacca
     8461 gtaggttagc aatgcgtaac gcgggcatat ccagtgcgcc gcagtggctg atacccgact
     8521 ggcgaaaacc gtgacgaccg ccatcctgca cagtggtata catgcccgca cgaataatct
     8581 tcagcatact ccctccttct gcggtacaaa gcgcacgctg tctcccggac gtaataagat
     8641 gggttcgtca cgcgccggat caaacaggct gagtgaggta tgaccaatca actgccagcc
     8701 acccggcgtt gccagcggat aaacaccggt ctgcggcccg ccgatcccga cagaacctgc
     8761 cggaacgagc aagcgcggtt cagcgcgccg tggcgtgtgt agttgttccg gcaaactccc
     8821 gagatacggg aagcccggtt gaaaacctaa aaaccagacc acgtattcca cggaggagtg
     8881 caattcaaca acctgttttt cgctcaaccc acaatgcgcc gcgaccaccg ccaaatccgg
     8941 tccgcctgcg ccaccgtaaa ccaccggaat ttcaataaaa cgagactccg gctccagcgc
     9001 ctcactctcc tcccaccagc gttgcaaacg ctctatggca tccagcgcca gcgactcagg
     9061 attacgtaaa atcaccgtga tattgttcat gccaggaatg gcttcaacca cattcggcat
     9121 atccaccaga cgctgcgcca gtcgccagat ccgtttctgg ctagccagcg tcaccggcgg
     9181 ttccagctcc agcactaccg ccgtttcacc tatcagataa caacgcgctc gttgcacttt
     9241 cgatccctct gattattcgt tatgcaggat taggaatatc aataaaggtc acatcaagat
     9301 cggtattttc attcagccac tcgctcaatg cgcgaatacc accgcgttca gtggcatggt
     9361 gacccgcagc ataaaaatgc aatccctgct cgcgggctga atgaatggtc tgttcagaaa
     9421 cttcgccagt aataaaagca tccacgccaa aacgcgcggc gctatcgata aaactttgcc
     9481 cgccgcccgt gcaccaggcg acgcgctgga ccacctcagg tccggtatcg ccacaccata
     9541 acggcttacg tcccagacgc gcttcaatcc aggaggccag ttccagtccc ggcacaggca
     9601 tggtcagttc gccccacggc accaacggct caatttcgcc catgaccgtg atccccagta
     9661 acgccgccag ctgtgcgtta ttgcccagct caggatgtgc gtcaagcggc aaatgccagc
     9721 catacaggtt gatatcattc gccagcaacg tttttaaacg gttacgcttc atgccgcgaa
     9781 tgaccgggga ttcgcctttc cagaagtagc catgatgcac aatgaccgca tccgcgccca
     9841 gacgcaccgc ttcatcgagc aaagcctggc tggcagtgac accggtaaca attttttgca
     9901 ccgtctcttt gccttccacc tgcaaaccat tcggcgcgta atcactaatc gccgcgctgt
     9961 tcagtttttc gttaatcagt tgttccagtt cggtgttttt catcttcgcg ccttccgtat
    10021 tctgatagct acaccgttaa catagcgtta cctcgcctgt tcgtcgatag ccaatctttt
    10081 gacatattaa gaaacaaacc cgacgccaaa agacgcaaat tgtttatatc atcacgcttc
    10141 agctaatgta tatttatgca atcagacagc atattgcata aataatcaga tctgtatcaa
    10201 agttttaact ttaaggaaac acaagcacgc cgcgcaacta ttacgtgacg ttgccatcaa
    10261 ttgtggaatt attatgaata aatacgcatc acaaccgcgc gctatttact atgtcgttgc
    10321 actgcaaatc tgggaatatt ttagctttta cggcatgcgt gccctgctga ttctctatct
    10381 caccaatcaa ctaaaataca acgatactca cgcctacgag ttatttagcg cctactgttc
    10441 gctggtgtat gtcacgccaa tcctcggtgg ctttttggcg gataaagttc tcggtaatcg
    10501 catggcggtg atgctggggg cgttgttgat ggcgatcggt catgtggtgc tgggtgccag
    10561 tgagatccat ccgtcattcc tctatctgtc cctggcgatt atcgtttgtg gctatggtct
    10621 gtttaaatcc aacgtcagtt gcctgctcgg cgagctgtat gagccaaccg atccgcgtcg
    10681 tgatggcggc ttctcgctga tgtatgcggc gggtaacgtg ggatctatta tcgcacctat
    10741 cgcctgtggc tacgcccagg aagagtacag ttgggcgatg ggctttggcc tggcggcggt
    10801 tggcatgatc gcgggtctgg tcattttctt atgtggcaat cgtcatttca cacacacccg
    10861 cggcgttaac aaaaaggtac tgcgtgcgac aaactttctc ctgcccaact ggggatggct
    10921 actggttctg ctggtggcaa cgcctgcgct gattaccgtg ttgttctgga aagagtggtc
    10981 ggtatacgcc ttgattgtcg cgacgatcat tggccttggg gttctggcta aaatttatcg
    11041 caaagcagaa aaccaaaaac agcgtaagga gctggggctg attgtgacgc tcaccttctt
    11101 cagtatgttg ttctgggcct tcgcacaaca gggcggtagc tcgattagcc tttatatcga
    11161 ccgcttcgtt aaccgcgata tgtttggtta taccgttccg accgcgatgt tccagtcaat
    11221 taatgccttc gcggttatgc tgtgcggtgt gttcctggca tgggtggtta aagaaagcgt
    11281 cgcaggtaat cgtaccgtgc gcatctgggg gaaatttgct cttggtctcg gcctgatgag
    11341 cgccggattc tgcattctga ccttaagcgc ccgctggtct gcaatgtatg gtcattcttc
    11401 tctgccactg atggtgttag gcctggcggt gatggggttt gccgaactgt ttatcgaccc
    11461 ggttgctatg tcgcagatta cgcgcattga aatccctggc gtgactggcg tattaactgg
    11521 catttatatg ctgctttccg gcgcgattgc gaactatctg gctggcgtga ttgccgatca
    11581 gacatcgcag gcttcgtttg atgcttccgg ggcgatcaac tactccatca atgcatatat
    11641 tgaagtattt gatcaaatta cctggggcgc actggcgtgt gtaggagtgg tgttgatgat
    11701 ttggctgtat caggcgctga aattcagaaa ccgcgcgctg gcgctggaat cttaatagac
    11761 cagcaccgat gcctgataag acgctcactg cgttttatca ggctcctttc ctcgcttctt
    11821 cgtacgctgc cagcgttcgc agccttgcct ctttgtgatc aactatcggt tgcggataat
    11881 ccagcgtcat acctgctttc tgcgcccact tccacggctc atgcaccact ttccctggca
    11941 catcgcgcag ttccggtagc cactggcgga taaactcgcc ttcgcggtca aatttctcgc
    12001 cctgggttgt cgggttaaaa atacgaaaat acggcgctgc atcggttccg gttgaagcgg
    12061 cccactgcca gccaccgtta ttagctgcca aatcaccatc aatcagctgc gacatgaaat
    12121 atcgctcgcc ttcgcgccag tcgatcaaca aatctttcac cagaaaactg gctgtaatca
    12181 tccgtagcct gttatgcatc cagccagtgc tgttaagctg acgcatggca gcatcgacaa
    12241 tcgggtatcc cgttttgcct tcctgccagg cctgtaaatg tgcgggattg ctctgccact
    12301 gcacacgatc cgtccaggca ataaacggac aatgtttaca caacgagggg taatacgtca
    12361 tcagatgacg gtagaactcg cgccagatca gctcactaag ccagacacta ccggccccac
    12421 cgtccagcgc ctgcggctgt tccgccaaca agcgatgcaa acactggcga ggcgataacc
    12481 cgcccgttgc caggctggcg gacaaacgac tggtgccttc cactgccgga aaatctcgtt
    12541 gttgctcata ttctccggca ccgttctggc aaaactggcg taattgcgca atcgcagctt
    12601 tttcttccac cgggaaatgc gcagtatcga aagactgacg aggataattc agcgtaatgg
    12661 atggcgcggg ctttatcgat ccgctactac gaacttttgg tgcagcgacg cactccggca
    12721 tcccttcccg cagccgtttc agccaggcat tttgaaaagg cgtaaagact ttgtacatct
    12781 catgattgcc agtcatcacc gcgccaggcg gcaggatcac gctgtcatca aatccttcac
    12841 acaccacgtt acgcagcgtt ctctcaacct gcacatcccg cgcccgctca ttcacctcat
    12901 actgatagtt ataaaaaaga tgagtaacgc tgttttccgc gcacacctgt ttaactattt
    12961 cgacgctggc ggcaaagtca tccacttcac ggaacaataa aggaatgcct ttttccgcca
    13021 gcgcgatttg tagcccgttc agttgagcat tgataagttc agcctgacgc ggcgacatgt
    13081 tatgcgccgc ccactggcgt ggtgtagcga tatacaacgc cagcacgctt gcagacgaat
    13141 tgcggcaggc ggcagccagc gcgagattat cgtgctgacg taaatcctgg cgaaaccaga
    13201 ccagatgggt agtcataaaa ctcctgaatg gcgcaagtta atcacacacg gccaaagttc
    13261 gaaataacgc tgcactgaaa gccaggcgtc aggataaagc gccatatagt gtttgatacg
    13321 catcagcggc gcaagaagtg gttgctcgcc acggcgataa ctgtcgataa gcgccgccag
    13381 ctgctggcgt tctgtggaat caatatgtgg gcgaaaatat ccctgaacgt gcatcaaaac
    13441 gttagtatga tcacgccggt tggcagggtg agaaagcaaa acaatcacgc gttggcggta
    13501 ctggttgtaa tagtcatcca gactttgcca ccggtgaata tcagccacaa acgggccaag
    13561 tttgcggtat tccggctggg aatgcgccag aaagacgagt ttataacggc tgtgataatc
    13621 gagaagcgcc ccgcgcgtta agccatgttc tttcagtcga tatagctcat aaatcgcgaa
    13681 gatgcggatt agggtgcttt catcaaatcg ttgctgattc attactgctc ctgaacggtt
    13741 tacaggagta agtgtagacg ttgggaaatg aaatttgtga agttaatcct gttgtagcag
    13801 ggcaagatta cgaaagcccg ctccccgtaa ggactgacgc caggtagttt ctgtccatag
    13861 ctgccctttc tattcaaacg tgaaatcaat aagttcatca gggacatcac aaacaatatg
    13921 aagacgggtt tcttctctgc cgtgactctt ttcactaatt gagtaactgt cgtgctctgg
    13981 attatttaat tctttcagcg gaaatttttc ctcaaaggct ttattaagcc gccacctgat
    14041 ttccttttac agcgaataaa taatcacctc cctgtttttg tatcttctct gcaatatctt
    14101 tctggcaact catcgcatca gttgtgatga tttttccttt aatatccagc atgttaagaa
    14161 gttcagggat agctgtaatt tcattg
//
LOCUS       DAPHCV010000115        14168 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.115, whole genome shotgun sequence.
ACCESSION   DAPHCV010000115 DAPHCV010000000
VERSION     DAPHCV010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.115"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(130..1488)
                     /gene="plaP"
                     /locus_tag="RH033_003753"
     CDS             complement(130..1488)
                     /gene="plaP"
                     /locus_tag="RH033_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HDV2992876.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1478..1540)
                     /gene="yoeI"
                     /locus_tag="RH033_003754"
     CDS             complement(1478..1540)
                     /gene="yoeI"
                     /locus_tag="RH033_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HDV2992877.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(1755..2684)
                     /locus_tag="RH033_003755"
     CDS             complement(1755..2684)
                     /locus_tag="RH033_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2992878.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(2730..3554)
                     /locus_tag="RH033_003756"
     CDS             complement(2730..3554)
                     /locus_tag="RH033_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV2992879.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDVDALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGMVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(3637..3891)
                     /gene="yoeB"
                     /locus_tag="RH033_003757"
     CDS             complement(3637..3891)
                     /gene="yoeB"
                     /locus_tag="RH033_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HDV2992880.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(3888..4139)
                     /gene="yefM"
                     /locus_tag="RH033_003758"
     CDS             complement(3888..4139)
                     /gene="yefM"
                     /locus_tag="RH033_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HDV2992881.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            4422..4472
                     /gene="hisL"
                     /locus_tag="RH033_003759"
     CDS             4422..4472
                     /gene="hisL"
                     /locus_tag="RH033_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HDV2992882.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    4448..4571
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            4618..5517
                     /gene="hisG"
                     /locus_tag="RH033_003760"
     CDS             4618..5517
                     /gene="hisG"
                     /locus_tag="RH033_003760"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDV2992883.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGICFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            5523..6827
                     /gene="hisD"
                     /locus_tag="RH033_003761"
     CDS             5523..6827
                     /gene="hisD"
                     /locus_tag="RH033_003761"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDV2992884.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETARQALSASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            6824..7894
                     /gene="hisC"
                     /locus_tag="RH033_003762"
     CDS             6824..7894
                     /gene="hisC"
                     /locus_tag="RH033_003762"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDV2992885.1"
                     /translation="MSTVTITDLARENVRNLMPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            7894..8961
                     /gene="hisB"
                     /locus_tag="RH033_003763"
     CDS             7894..8961
                     /gene="hisB"
                     /locus_tag="RH033_003763"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HDV2992886.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKLDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKVFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            8961..9551
                     /gene="hisH"
                     /locus_tag="RH033_003764"
     CDS             8961..9551
                     /gene="hisH"
                     /locus_tag="RH033_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDV2992887.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            9551..10288
                     /gene="hisA"
                     /locus_tag="RH033_003765"
     CDS             9551..10288
                     /gene="hisA"
                     /locus_tag="RH033_003765"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDV2992888.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGANALVLALDVRIDEQGNKQVAVSGWQENSGISLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDIDDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            10270..11046
                     /gene="hisF"
                     /locus_tag="RH033_003766"
     CDS             10270..11046
                     /gene="hisF"
                     /locus_tag="RH033_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDV2992889.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            11040..11651
                     /locus_tag="RH033_003767"
     CDS             11040..11651
                     /locus_tag="RH033_003767"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDV2992890.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPETLDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(11748..12725)
                     /gene="wzzB"
                     /locus_tag="RH033_003768"
     CDS             complement(11748..12725)
                     /gene="wzzB"
                     /locus_tag="RH033_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HDV2992891.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLIQLWRGKMTIIISVIVAIAL
                     AIGYLVVAKEKWTSTAIVTQPDVGQIAGYNNAINVIYGSAAPKVSEIQSMLIGRFSTT
                     FSALAETLDNQEEPEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAQYIQQVDDQVN
                     EELEQDLKDNIALQMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQIQ
                     QTQDVTQDTMFLLGSEALESMIKHEATRPLVFSSNYYQTRQNLLDIDNLDVDKLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(12872..14038)
                     /gene="ugd"
                     /locus_tag="RH033_003769"
     CDS             complement(12872..14038)
                     /gene="ugd"
                     /locus_tag="RH033_003769"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HDV2992892.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYVVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
BASE COUNT         3612 a         3640 c         3579 g         3337 t
ORIGIN      
        1 ggaatgcaga ccaataacca aattctctaa attagaacaa atggttatta atgaggatgt
       61 cgataaaaaa gaataaaaaa accggagccc aatattcatt cgactccggt ttattattac
      121 aaaagatgat tacgctacgt cttcgtactg cggaaccgga ttacggaaac tcttggtgac
      181 gcaagccagg taaatcagac cgatagccgc ccagatcaga cccagaacca ttgagctttc
      241 ttccaggtta acccacagcg caccaacggt cagcgcacca cacatcggca ggaacagata
      301 ctggaagtga tctttcagcg tcttgttacg cttctcacgg atccagaact gcgagatgac
      361 cgacaggtta acgaaggtga acgccaccaa cgcaccaaag ttgatcagcg ctgtagccat
      421 taccaggtcg aagttgattg ccagcaaggc aatcgcgcca accaggatga tgttcatcgc
      481 cggagtacgc catttcgggt gtacataacc gaagaagctt ttcgggaata cgccgtcacg
      541 ccccattacg tacatcagac gcgcaacgcc tgcatgcgcc gccataccgg acgccagtac
      601 ggtaatggtg gagaagatca gcgcaccaac ctggaatgct ttacctgcaa catacagcat
      661 gatttcaggc tgtgacgcat ccggatcttt aaagcgagag atatccggga agtacagctg
      721 caggaagtaa gttgcaaaga tgaagatcat gccgccaatc agcgcggtca ggaaaatcgc
      781 acgcgggatc acgcgctctg catctttggt ttcttccgac aggttgctga tgccgtcaaa
      841 gccggtaaag gagaagcaca ggattgtcgc cccggtaatc atcgggataa catgcgcatc
      901 gccagaccag aatggacgag tgctcgccag cgtaccagcg ccttcacctt caaatacgcc
      961 ataaacaacc atgcccagaa tcaccgcgat cagcactacc tgcaacacga cgattacagt
     1021 gttgaagttc gctacggatt tcagactacg caggttaaag gcggtcataa aggccaccag
     1081 cgccaccaca aacatccacg atgggatgga aggcaccaga gcttcaaaat aaattttcgc
     1141 cagcagaatg ttgatcatcg gcgcgaacag atagtcgagc agagaagacc aacccaccat
     1201 aaagccgaca gtcgggctaa tggatttctg ggcgtaagtg tatgcagagc cagcagaagg
     1261 atagcggcga accagcttcc cgtagctcag agccgtaaac aggatcgcaa tcaatgcgaa
     1321 cgcataggct gtcggcacat gaccatccgt gaggcctgaa acgataccaa aagtatcaaa
     1381 cagcgtcatc ggctgcatat aggcaagacc catcattaca accggaacta acgtaagcgt
     1441 tttacgcaat tccacgcgag aggtgtttgg agtaacgtta tgcgacatgg tcattctcct
     1501 ttacggtgat aaccgtcgcg taagcaaaaa attgccccat ttttttggat tcctcagcga
     1561 caacaactgt cgatttttag taaatatcta tccggtacga agcccggcct cttggtatga
     1621 atgattggtt tgaagcaaaa aataaccgac gctgatgaaa cgtcggtttt tagtcatttt
     1681 ttgacagcgg cgcattgtgc ctaaatgggg gggaaatgac aagagaatga gaggcttgtc
     1741 ataataattt ttctttaaat ggctgatttt ccgtcatcag attttgcata aacaccggcg
     1801 atcgcactat ttgctaaaat ttcatctcgc caccagccgc tggcaaggcc tgcggtctgt
     1861 tcgttccagc caatccagac gggttcaaag agagtttggg cgaccacttt cttctcgatt
     1921 aacgcgccac tatcgagaaa ccgttgcgcc agataacggg gtaaatagcc gcagccgagg
     1981 ccgctaattt gcagctctag cttggtttta aaatcgaaga cggtaatggc ttcttgttcg
     2041 tcgagaagtt gtgaggccgt agaagcgccg gcctgcgcag tgtcccccac cacaatcgcc
     2101 cgatatcgct taatgatacg acggtttaac ggttcttctt ccagggctaa cggatgatga
     2161 ggcgctaccg caaagacttg ttccagatcg cccaaccgcg agaaaccaaa ctcactggaa
     2221 gatggtggct catgcatcgc gccaacaata atatccgctc gcccctgagt cagggcatcc
     2281 catgaaccac cgagtacgcc attgataaac ttcaggcgcg tcacgctatg atgttgataa
     2341 aaagcttcaa tgagtggcgc aagcagagaa aaaggaaacg tgtcgtccac gccaatcacc
     2401 agttcgtttt cccagccttc atgcagtttg atcgcctgct tttccagttc tcgcacggta
     2461 tgcagaactt cccgcccttt ctctaataac atttttccgg tgcgggtgaa tttagcgcgg
     2521 tgtccgctac gatcaagcaa ttgaatatta aggtcgcttt ccagcttgtg aacggtgtaa
     2581 ctcagagcgg acggcgtctt atagagtttg gccgacgccg ccgcaaaact gccttctttt
     2641 tctaaggcat cgaggatcat caacacatcc agcagtggtt tcatacacgc ccccttgcag
     2701 tgcatgcggc gtcctgctgg cgtggtcgtt tactccagcg gcattaccag cggatcggga
     2761 tactggtatt caaatcccag ttcattacaa atccgactgc catcaataat cttacctttg
     2821 ccgctgtcca gactatttct gaactgcgga ggctccagcc ccagtaaacg ggccatctgc
     2881 ggatagaaaa cattacgcgc agggtgagct ggcgcacata tattatagat gtgtccgcct
     2941 ttaggtgcct gtaacaacag agtgatagcg ccaataacat cttctaaatg gactaaatta
     3001 acaccatgtt caccatcagg cgcggttttt ccggcaaaga agcgtccggg atgacgcccc
     3061 ggtccgacca ggcccgcaag acgcagaata tcgaccgaag taccgggtaa attgtgcagc
     3121 cagtcttcga gttcttctaa tactcgtcca ctgttggtta ccggattacg cggggtagtt
     3181 tctttcacca tgccttgcgc atcgccatag acagatgttg agctggtaaa aataattcgg
     3241 ggaatacgat gggccagcgc gctatccact aactcttgta ccgcttgtaa atagaactca
     3301 tcgccggggc cgctacgacg tgccggaagc gtaatgacca gcgcatcgac atccatcagg
     3361 gcatccagat cgtcagaatc gcaaactaac tcaggctcca tgcgaagcag atagctatca
     3421 atgccactca ttcgggccgc ttcgacacca tcttgtgtgg ttttactccc ggtgacttgc
     3481 cagcctcgcg ctgaaagtga catcgccagc ggcatgccta accaccctaa cccgacaatt
     3541 gcgacctttt tcatccgtta tctcctgata caccttagat ctataaggct acgctagcgt
     3601 atcaaaactg acaattcatt ctatgaatga atctgttcaa taatgataac gacatgctgc
     3661 aatgagcagt gaatcgtcgg taaccgcgta taccagacgg tgctcctctg taatgcgtcg
     3721 ggaccagaaa cctgacaaat tatgtttcag gggttctggc ttccccttac cttcaaatgg
     3781 cgttctgcgg gtatctttga taagttcatt gatcttttta acaattcgct tatctgtttc
     3841 ctgccagtac agataatcat cccatgattc ctcagaccag attagtttca ctcaataatg
     3901 tccttttctg ttcctttgcc tgatttcagg ctatcgattg agtccatcaa tctccgggcg
     3961 ttagcggggg aacgcagtag ataagccgtc tcttccagcg agttgtattc ttcgagtgac
     4021 atcagaacac aagcctctcc attctgacga gtaataagga tcggggcatg atcttcaacg
     4081 gctttcatca ttgttgccga caaattctga cgcgcttcgc tgtagctaat tgtacgcatg
     4141 tcaatctcct cttttgtaca gttcattgta caatgatggg cgttaattaa ctatttatta
     4201 attagtttgt agatcaaggt attgtcagtg agacgaaaat ccaggcttcg ctatttttgg
     4261 tgccgtcagc ttagaggaca gtcctcttag cgccctcctt tccccgctca ttcattaaac
     4321 aaatccattg ccataaaata tataaaaaag cccttgcttt ctaacgtgaa agtggtttag
     4381 gttaaaagac atcagttgaa taaacattca cagagacttt tatgacacgc gttcaattta
     4441 aacaccacca tcatcaccat catcctgact agtctttcag gcgatgtgtg ctggaagaca
     4501 ttcagatctt ccagtggtgc atgaacgcat gagaaagccc ccggaagatc accttccggg
     4561 ggctttttta ttgcgcggtt gctaacggtt cagacaggtt taaagaggaa taagaaaatg
     4621 acagacaaca ctcgtttacg catagctatg cagaaatccg gccgtttaag tgatgactca
     4681 cgtgaattgc tggcgcgctg tggcattaaa atcaatcttc acacccagcg cctgatcgcg
     4741 atggcagaaa acatgccgat tgatattctg cgcgtgcgtg acgacgacat tcccggtctg
     4801 gtaatggacg gcgtggtaga ccttggaatt atcggcgaaa acgtgctgga agaagaactg
     4861 cttaaccgcc gcgcccaggg tgaagatccg cgctacttta ccctgcgtcg actggatttc
     4921 ggcggctgcc gactttcact ggcaacgccg gttgatgaag cctgggatgg cccgctctcc
     4981 ttaaacggta aacgtatcgc cacctcttat cctcacctgc tcaagcgtta tctcgaccag
     5041 aaaggtatct gttttaaatc ctgcttactg aacggttctg ttgaagtcgc cccgcgtgcc
     5101 ggactggcgg atgcgatttg cgatctggtt tccaccggtg ccacgctgga agctaacggc
     5161 ctgcgcgaag tcgaagttat ctatcgctcg aaagcctgcc tgatccagcg cgatggcgaa
     5221 atggaagaat ccaaacagca actgatcgac aaactgctga cccgtattca gggtgtgatt
     5281 caggcgcgcg aatcaaaata catcatgatg cacgcaccga ccgaacgtct ggatgaagtc
     5341 atcgccctgc tgccaggtgc cgaacgccca accattctgc cgctggcggg tgaccaacaa
     5401 cgcgtagcga tgcacatggt cagtagcgaa accctgttct gggaaacgat ggaaaaactg
     5461 aaagcgctgg gtgccagttc aatcctggtc ctgccgattg agaagatgat ggagtgatcg
     5521 ccatgagctt taacacaatc attgactgga atagctgtac tgcggagcaa caacgccagc
     5581 tgttaatgcg cccggcgatc tccgcttctg aaagcattac ccgcactgtt aacgatattc
     5641 tcgataacgt gaaagcacgc ggcgatgagg ccctgcggga atacagcgcg aagtttgata
     5701 aaaccacggt taccgcactg aaggtgtctg ctgaggaaat tgccgccgcc agcgaacgcc
     5761 tgagcgacga gctaaaacag gcgatggcgg tggcagtaaa gaatattgaa accttccaca
     5821 ctgcgcaaaa actgccgccg gtagatgtag aaactcagcc aggcgtacgt tgtcagcaag
     5881 tcacgcgccc ggtggcttcg gtaggtttgt atattcctgg cggctccgcc ccgctcttct
     5941 caacggtatt aatgttggca actccggcgc gtattgcggg ctgtaaaaaa gtggtgctgt
     6001 gctcaccgcc gccgattgcc gatgagatcc tttatgcggc gcagctgtgc ggtgtacagg
     6061 acgtgtttaa cgtcggcggc gcacaggcca ttgccgcact ggcgtttggt actgaatctg
     6121 tgccaaaagt ggacaaaatc ttcgggccag gcaacgcctt tgtcaccgag gcaaaacgcc
     6181 aggtaagcca gcgtctggac ggtgcggcga tcgatatgcc cgcaggcccg tcggaagtgc
     6241 tggtgattgc cgacagcggc gctacgccag atttcgtggc ttctgatttg ctttctcagg
     6301 ctgaacacgg cccggactct caggtgattt tactgacgcc cgatgctgat atggcgcgtc
     6361 gcgttgccga agctgtcgaa cgccagctgg cagaactgcc gcgtgccgaa accgcacgtc
     6421 aggcactgag cgccagccgc ctgatcgtga ctaaagattt agcgcagtgc gtagagattt
     6481 ccaaccagta cggcccggag cacctgatca ttcagacccg caacgcccgc gaactggtcg
     6541 atggcattac cagtgccggt tcggtatttc ttggtgactg gtcaccggaa tcggcaggtg
     6601 attacgcctc cggcaccaac cacgttctgc cgacttacgg ttacaccgcc acctgttcca
     6661 gcctcgggct ggcggatttc cagaagcgca tgaccgtgca ggaactgtcg aaagaaggtt
     6721 tctccgcgct ggcttcgacc attgaaacac tggccgccgc cgagcgcctg accgcccaca
     6781 aaaatgccgt tactttgcgt gttaacgccc ttaaggagca agcatgagca ccgtgactat
     6841 taccgattta gcgcgtgaaa acgtccgcaa cctgatgccg tatcagtcgg cgcgtcgtct
     6901 gggcggtaac ggcgacgtct ggctgaacgc caacgaatat cccacagccg tggagtttca
     6961 gctcactcaa caaacgctca accgctatcc ggaatgtcag ccaaaagcgg tgattgaaaa
     7021 ttacgcgcaa tatgcaggcg taaaaccgga gcaggtgctg gtcagccgtg gcgcggacga
     7081 aggtattgaa ctgctgattc gcgctttttg cgaaccaggt aaagacgcca tcctctactg
     7141 cccgccaacg tacggcatgt acagcgtcag cgccgaaacg attggcgtcg agtgccgcac
     7201 agtgccgacg ctggacaact ggcaactgga cttacagggc atttccgaca agctggacgg
     7261 cgtaaaagtg gtctatgttt gcagccccaa caacccgacc gggcaactga tcaacccgca
     7321 ggattttcgc accctgctgg agttaacccg cggtaaggcg attgtggttg ccgatgaagc
     7381 ctatatagag ttttgcccgc aggcatcgct ggctggctgg ctggcggaat atccgcacct
     7441 ggctattttg cgcacactgt cgaaagcttt tgctctggcg ggccttcgtt gcgggtttac
     7501 gctggcaaac gaagaagtca tcaacctgct gatgaaagtg atcgccccct acccgctctc
     7561 gacgccggtt gccgacattg cggcccaggc gttaagcccg cagggaatcg tcgccatgcg
     7621 cgaacgggta gcgcaaatta ttgcagaacg cgaatacctg attgccgcac tgaaagagat
     7681 cccctgcgtg gagcaggttt ttgactctga aaccaactac attctggcgc gctttaaagc
     7741 ctccagtgca gtgtttaaat ctttgtggga tcagggcatt atcttacgtg atcagaataa
     7801 acaaccctct ttaagcggct gcctgcgaat taccgtcgga acccgtgaag aaagccagcg
     7861 cgtcattgac gccttacgtg cggagcaagt ttgatgagtc agaagtatct ttttatcgat
     7921 cgcgatggaa ccctgattag cgaaccgccg agtgattttc aggtggaccg ttttgataaa
     7981 ctcgcctttg aaccgggcgt gatcccggaa ctgctgaagc tgcaaaaagc gggctacaag
     8041 ctggtgatga tcactaatca ggatggtctg ggaacacaaa gtttcccaca ggcggatttc
     8101 gatggcccgc acaacctgat gatgcagatc ttcacctcgc aaggcgtaca gtttgatgaa
     8161 gtgctgattt gtccgcacct gcccgccgat gagtgcgact gccgtaagcc gaaagtaaaa
     8221 ctggtggaac gttatctggc tgagcaagcg atggatcgcg ctaacagtta tgtgattggc
     8281 gatcgcgcga ccgacattca actggcggaa aacatgggca ttaatggttt acgctacgac
     8341 cgcgaaaccc tgaactggcc gatgattggc gagcaactca ctaaactaga ccgttacgcc
     8401 catgtagtgc gcaacaccaa agagacgcaa attgacgtcc aggtgtggct ggatcgcgaa
     8461 ggtggcagca agattaacac cggcgttggc ttctttgatc acatgctgga tcagattgcc
     8521 acccacggcg gcttccggat ggaaatcaac gtcaaaggcg acctctatat cgacgatcat
     8581 cacaccgtcg aagataccgg cctggcgctg ggcgaagcgt taaaaattgc cctcggcgac
     8641 aaacgcggta tttgccgctt tggttttgtg ctgccgatgg acgaatgcct tgcccgctgc
     8701 gcgctggata tctctggtcg cccgcacctg gaatataaag ctgaatttac ctaccagcgc
     8761 gtaggcgatc tcagcactga aatgatcgag cacttcttcc gttcgctgtc ttacaccatg
     8821 ggcgtgacgc tacacctgaa aaccaaaggt aaaaacgatc atcaccgtgt agagagcctg
     8881 ttcaaagtct ttggtcgcac cctgcgccag gccatccgcg tggaaggcga caccctgccc
     8941 tcgtcgaaag gagtgctgta atgaacgtgg tgatccttga tactggctgc gccaacctga
     9001 actcggtgaa gtcagccatt gcgcgtcacg gttatgaacc caaagtcagc cgtgacccgg
     9061 acgtcgtgtt gctggccgat aaactatttt tacccggcgt tggcaccgca caagcagcga
     9121 tggaccaggt gcgtgagcgc gagctgtttg atctcatcaa agcctgtacc caaccggtgc
     9181 tgggcatctg cttagggatg caactgctgg ggcggcgcag cgaagagagc aacggcgtcg
     9241 atttgctggg catcatcgac gaagacgtgc cgaaaatgac cgactttggt ctgcccctgc
     9301 cgcatatggg ctggaaccgc gtttacccgc aggcaggcaa ccgcctgttt caggggattg
     9361 aagacggcgc gtacttttac tttgttcaca gctacgcaat gccggtcaat ccgtggacca
     9421 tcgcccagtg taattacggc gaaccgttca ccgcggcggt acaaaaagat aacttctacg
     9481 gcgtgcagtt tcacccggag cgttctggtg ccgctggcgc taagttgctg aaaaacttcc
     9541 tggagatgtg atgattattc cggcattaga tttaatcgac ggcaccgtgg tacgtctcca
     9601 tcagggcgat tacggcaaac agcgcgatta cggtaatgac ccgctgccgc gcttacagga
     9661 ttacgccgcg cagggtgccg aagtgctgca cctggtggat ctgaccgggg caaaagatcc
     9721 ggcaaaacgt caaatcccgc tgattaaaac cctggtggcg ggcgttaacg taccggtgca
     9781 ggttggcggc ggtgtgcgta gcgaagaaga cgtggcagca ttactggaag caggcgttgc
     9841 gcgcgtggtg gttggctcca ccgcggtgaa atcaccagaa atggtgaaag gctggtttga
     9901 acgtttcggt gccaatgcct tagtgctggc gctggatgtc cgtatagacg agcaaggcaa
     9961 caagcaggtt gcagtcagcg gctggcaaga gaactcgggc atttcactgg aacaactggt
    10021 ggaaacctat ctgcccgtcg gcctgaaaca tgtgctgtgt accgatatct cgcgcgacgg
    10081 cacgctggca ggttctaacg tctctttata tgaagaagtg tgcgccagat atccgcaagt
    10141 ggcatttcag tcttccggcg gcattggcga cattgatgat gtcgcggccc tgcgtggcac
    10201 tggtgtgcgc ggcgtaatag ttggtcgtgc attactggaa ggtaaattca ccgtgaagga
    10261 ggccatcgca tgctggcaaa acgcataatc ccatgtctcg acgttcgtga tggtcaggtg
    10321 gtgaaaggcg tacagtttcg caaccatgaa atcattggcg atatcgtgcc gctggcaaaa
    10381 cgctacgctg aagaaggtgc agacgaactg gtgttctacg atatcaccgc ttccagcgat
    10441 ggccgtgtgg tagataaaag ctgggtatct cgcgtggcgg aagtgatcga cattccgttt
    10501 tgtgtggcgg gtgggattaa gtctctggaa gatgccgcga aaattctttc ctttggcgcg
    10561 gataaaattt ccatcaactc ccctgcgctg gcggacccga cgttaattac tcgcctggcg
    10621 gatcgatttg gcgtgcagtg tattgtggtc ggtattgata cctggtacga cgctgaaacc
    10681 ggtaaatatc atgtgaatca atataccggc gatgaaagcc gcacccgcgt cactcagtgg
    10741 gaaacgcttg actgggtaca ggaagtgcaa aaacgcggtg ccggagaaat cgtcctcaat
    10801 atgatgaatc aggacggcgt gcgtaacggt tacgacctcg aacagctgaa aaaagtgcgt
    10861 gaagtttgcc acgtcccgct gattgcctcc ggtggcgcgg gcaccatgga acacttcctc
    10921 gaagccttcc gcgatgccga cgttgacggc gcgctggcag cttccgtatt ccacaaacaa
    10981 ataatcaata ttggtgaatt aaaagcgtac ctggcaacac agggcgtgga gatcaggata
    11041 tgttaacaga acaacaacgt cgcgaactgg actgggaaaa aaccgacgga cttatgccgg
    11101 tgattgtgca acacgcggta tccggtgaag tgttaatgct gggctatatg aacccggaaa
    11161 ccttagacaa aaccatcgaa agcggcaaag tcaccttctt ctcgcgcact aaacagcgac
    11221 tgtggaccaa aggcgaaacg tcgggcaatt tcctcaacgt agtgagtatt gccccggact
    11281 gcgacaacga cacgctactg gtactggcga atcctatcgg accaacctgc cacaaaggca
    11341 ccagcagctg cttcggcgac accgctcacc agtggttgtt cctgtatcaa ctggaacaac
    11401 tgctcgccga acgcaaatct gccgacccgg aaacctctta cactgccaaa ctgtatgcca
    11461 gcggcaccaa acgcattgcg cagaaagtgg gcgaagaagg cgtggaaacc gcactggccg
    11521 cgactgtaca tgaccgcttt gagctgacca acgaggcatc tgacttgatg tatcacctgc
    11581 tggtgctgtt gcaggatcag gatctggact taacgacggt gattgagaac ctgcgtaaac
    11641 gtcatcagtg agttgcgggg taagcggatg cgatgttgtt gtcgcatccg gcaaaaaaaa
    11701 cgggcaaggt gtcaccaccc tgcccgtttt ctttaaagct gcaaagatta cttcgcgttg
    11761 taattacgca gagcattacg ccccaacaca atccccgcgc caaccatgcc gcccagcagc
    11821 accgccagaa tcaaagtaat tgcctttttc gggctatcgc gacgaatagg taacgtcggt
    11881 ttcatcacat agcggtaagc atgaatatca agtttatcaa cgtcaagatt gtcgatatcc
    11941 agcaggtttt gacgagtctg atagtagttt gatgagaaca ccaacggacg agtcgcctcg
    12001 tgcttaatca tcgactccag cgcttcgctc cccaaaagga acatagtatc ttgggttacg
    12061 tcctgtgtct gctgaatctg cggctttgtc acctgcgcct gattcgcata ctgcaacgct
    12121 tcctgaatct gacggatacg cagatctttc tgctcctgcg caaccacttc ctgggtcttc
    12181 agagaatcct gcaagttttt catttgcagc gcgatgttgt ctttcaggtc ttgttccagt
    12241 tcttcattta cctggtcgtc aacttgctgg atatactgcg ccagctgttt ctgcgcacct
    12301 tcaggtgttt cacccacata agaaaccgct aaaggtaatg actgattttt aacggtaggc
    12361 tcaatggtca gtttttcagg ttcttcctga ttatccagcg tttctgctaa tgcagaaaat
    12421 gtcgtgctga aacgaccaat taacatcgac tggatctcag acactttcgg cgctgccgaa
    12481 ccataaatca catttatggc attgttataa ccagcgattt gccccacatc aggttgagta
    12541 acgatagctg tagaagtcca cttttctttg gcaacgacca gataccctat agccagggca
    12601 atagccacaa tgacagaaat gatgatagtc atcttgccac gccatagttg gatcagtaaa
    12661 tcaatcaaat caatctgttc cggatcatgg ttttgcccag aaacattatt attttctact
    12721 ctcatcatta ccctaacgga cgaaataatt ttaagtcgcc atagtttagc gataatcgcc
    12781 atgaatgcta taggataaat gataaaaaca atgaattatt acttagagaa cataagagaa
    12841 tcagggcgaa aatcgccctg ataacaagat gttagtcact gccaaagaga tcgcgggtgt
    12901 agactttatc tgccacatcc ttaagctctt ctgccatacg gttggaaata atgacgtcgg
    12961 cttgttgttt gaaggtggcg agatcacgtt ccagacgaga gttgaagaat gagtcttctt
    13021 tcatcaccgg ctcgtagatg atcacttcaa cgcctttcgc cttaatacgt ttcataatcc
    13081 cctgaatgga agacgcgcgg aagttatctg aaccgctctt cataatcaga cgataaatac
    13141 ccaccacttg cggcttgcgt gacaaaatgg catcggcaat aaagtcttta cgcgtgcggt
    13201 ttgcatcgac aattgccgag atcaggttat tcggcacaga ctggtagttc gccagtaact
    13261 gcttggtatc tttcggcaga caataaccac cataaccaaa cgacggattg ttgtaatggt
    13321 tgccaatacg cggatcgaga caaacgcctt cgataatctg gcgagtattc agacctaaac
    13381 tttctgcata gctatccagc tcattaaagt acgctacgcg catcgccaga taggtattag
    13441 caaaaagttt aatcgcttct gcttcagtgg agtcggtaaa cagggtcggg atattttgct
    13501 taatcgcgcc ttcctgtaac aacgcagcaa aacgttctgc gcgttctgaa cgctcaccaa
    13561 tgacaatacg tgacggatgg agattatcgt aaagagcttt accttcacgg agaaattcgg
    13621 gtgagaaaat aatattttca gtacgatatt tcttatgcat cgctgcggta aaaccaacgg
    13681 gaaccgttga tttgatgacc ataaccacat aaggatttat ctcaactacg tctttaatta
    13741 ctgattctac actggatgta ttgaaataat tagttttagg atcatagtcg gttggagtgg
    13801 cgatgatgac gtaatcagca tcccggtagg cttcattttt atctaatgtg gcattaaagt
    13861 gtattttatc tgattgcaaa aactgctgaa tttccttatc aacaatagga gatatccgat
    13921 cattcagcat agcaacgcgt gacggtaaaa tatctaatgc cacaacctca tgattttgtg
    13981 cgattagaag cccgtttgac aagcctacat agccagtacc ggaaatggtg attttcattt
    14041 catgctctca gaattaactt aacagtgaat catgatgttt ttagcatcct gataagagct
    14101 aaaagtttta acgctacgtt gtcagatggt caacgcaacg aaaaacaatt cagattttct
    14161 ttataaga
//
LOCUS       DAPHCV010000116        13891 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.116, whole genome shotgun sequence.
ACCESSION   DAPHCV010000116 DAPHCV010000000
VERSION     DAPHCV010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13891
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.116"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(36..896)
                     /gene="pdxI"
                     /locus_tag="RH033_003770"
     CDS             complement(36..896)
                     /gene="pdxI"
                     /locus_tag="RH033_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HDV2992893.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLGALDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKITEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(1127..1717)
                     /gene="paaY"
                     /locus_tag="RH033_003771"
     CDS             complement(1127..1717)
                     /gene="paaY"
                     /locus_tag="RH033_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HDV2992894.1"
                     /translation="MPIYQIDGLTPFVPEESFVHPTAVLIGDVILGKGVYVGPNTSLR
                     GDFGRIVVKDGANIQDNCVMHGFPDQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRSKQTLHQVEPLREVEPGRQRLVFDENLRPKQ"
     gene            complement(1699..1881)
                     /locus_tag="RH033_003772"
     CDS             complement(1699..1881)
                     /locus_tag="RH033_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaX"
                     /protein_id="HDV2992895.1"
                     /translation="MRDYQTFIGKLYQRVAPAALAFVSEKGETSVGELPAPGSLYFQR
                     FGGLNIEQEALCQFIR"
     gene            1846..2058
                     /locus_tag="RH033_003773"
     CDS             1846..2058
                     /locus_tag="RH033_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV2992896.1"
                     /translation="MKFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGF
                     SDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(2230..2556)
                     /locus_tag="RH033_003774"
     CDS             complement(2230..2556)
                     /locus_tag="RH033_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HDV2992897.1"
                     /translation="MKRTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVAL
                     KTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVERAKPGEYVQGING
                     KWVRKF"
     gene            complement(2564..2749)
                     /locus_tag="RH033_003775"
     CDS             complement(2564..2749)
                     /locus_tag="RH033_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HDV2992898.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(2746..5385)
                     /locus_tag="RH033_003776"
     CDS             complement(2746..5385)
                     /locus_tag="RH033_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HDV2992899.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQAMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVAAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPMQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWSE
                     DEPLLLTDVSVDVLGGNVLMKQLRMPQHDSALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNAHLPGNDCPQGKECEDKQ"
     gene            5593..6582
                     /gene="ldhA"
                     /locus_tag="RH033_003777"
     CDS             5593..6582
                     /gene="ldhA"
                     /locus_tag="RH033_003777"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDV2992900.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            6693..7115
                     /gene="hslJ"
                     /locus_tag="RH033_003778"
     CDS             6693..7115
                     /gene="hslJ"
                     /locus_tag="RH033_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HDV2992901.1"
                     /translation="MKKVAALVALSLLMAGCVSSDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(7112..7378)
                     /locus_tag="RH033_003779"
     CDS             complement(7112..7378)
                     /locus_tag="RH033_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HDV2992902.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            7652..11176
                     /gene="nifJ"
                     /locus_tag="RH033_003780"
     CDS             7652..11176
                     /gene="nifJ"
                     /locus_tag="RH033_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDV2992903.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAASVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILELMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRNVAVLDRTKESGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSAAEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDAKPEVRREQVAALRQQLKDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            11542..12675
                     /gene="ompN"
                     /locus_tag="RH033_003781"
     CDS             11542..12675
                     /gene="ompN"
                     /locus_tag="RH033_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HDV2992904.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSTNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            12816..13250
                     /gene="uspF"
                     /locus_tag="RH033_003782"
     CDS             12816..13250
                     /gene="uspF"
                     /locus_tag="RH033_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HDV2992905.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIRKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
BASE COUNT         3560 a         3551 c         3288 g         3492 t
ORIGIN      
        1 aaaagggaaa tgcatctttc ccttttgtat attcgttatt ctcgcgaaat accgtccaac
       61 gtagacaaca cttcctcaga aagatgcaat ttttcagcag ccatattctc ccgtaaatgc
      121 gcaacagaag acgtccctgg gatcagcaaa atattcggtg aacgctgtaa cagccacgcc
      181 agcgccacct gcattggtgt tgcgcccagg ctcgctgcta catccgaaag tgtggaggat
      241 tgcagcggcg taaagccccc aagcggaaag aacggcacgt aggcaatgcc gtcacgagcc
      301 aaagcatcaa tcattgcgtc atcagcacga tgagcaatgt tgtattcgtt ctgcacacag
      361 acgatttcgg taatcttacg cgcctctgca acctgcgtcg gtgtaacgtt gctcagacca
      421 atatgtttta ccaggccttg ttgttgcatc tctgccagta cggtcaggct ggcttcaatc
      481 gatccttccg ctgggccatg accatccccc atcataacgc gcaggttaac cacatccagt
      541 gcgcccagcc cgagattacg tagattatcg tgcaccgctt tttgcagctc tgccggagaa
      601 aatgcgggca accaggatgc gtcctctcca cgccgcgcac caattttagt gacaattgtc
      661 aggtcgtcag agtaaggata aagcgcttcg cggataatct gattggtgac gtgcggacca
      721 taaaagtcgc tggtatcaat atgattgacg cccaatgcca gcgcctcacg cagcacggtt
      781 atagcgacgt ggcgatctct tggggggcca aaaaccccag gacctgccag ttgcatcgcg
      841 ccataaccaa gacggttaac ggattttgta ccgagagtaa atgtattgct gctcatcgca
      901 ccctgccctg taataaaacg gattaagaaa aatagtatag aaacgaaaag agataaataa
      961 tgcgaaggcg taaacaagat tactggatga gttgctctat tgccctgaaa gacaccaaaa
     1021 caaaacgcgt caagtaccgc atccggcaat aaaaaaaccg caacctgtat cagcgttgcg
     1081 gtttaggtat cacatgctaa aaattagaac gtccagcatc aggacgttac tgtttcggtc
     1141 gcagattctc atcaaatacc aggcgctgcc tgccaggttc aacttcccgc aatggctcaa
     1201 cttgatgcaa cgtctgctta ctgcgtgtca ccagcacctg atactcatgc gtgccctgct
     1261 ttttccatgc cagttcctgc tcactaagtt cacgaatcgc ttttgccggg ctgccgacaa
     1321 tcaggtaatt agcaggcatt tctgctttgg ctttcacaaa tgcggaagcg ccaacaatgc
     1381 tgttctcgcc aatcaccgca ccgtccatca ctaccgcgtt catacccact aatgcattgc
     1441 ggcggataat gcagccgtga aggatagcgc tatgaccaat atgcccatct tcttccacga
     1501 cagtatcctg atcgggaaaa ccgtgcataa cgcaattatc ctgaatgttc gcgccatctt
     1561 tcaccacgat acgaccgaaa tcgccacgca ggctggtatt tggcccaacg tacacgccct
     1621 tgccgagaat aacatcgccg atcaataccg ctgtcggatg gacaaaactc tcttctggca
     1681 caaacggagt cagaccgtct atctgataaa ttggcataac gcctcctgtt caatattcaa
     1741 gccgccaaaa cgttgaaaat acaggcttcc cggcgcaggc aattcaccga ccgaggtttc
     1801 acctttttca ctaacgaacg ctaaagcagc aggcgctacg cgctgataaa gtttgccgat
     1861 aaaggtttgg tagtcgcgca atatatccgt aaccgtcgtc tcgatttttg tgcagatgcg
     1921 attcgccatg ccgcagatga tgaaaaactg gcaggcattg gctttcattg gggattttcc
     1981 gaccagagtc atttttcaac ggtatttaag caacgctttg ggatgacgcc aggcgagtat
     2041 cgacgtaaat tccgctaact aattcgcgtt gcatgtacag caacgcgaat tagttaaaac
     2101 agatatttga ctatgcattt ttcagggcga aaggcaattt gacaggaatt tttgaatatt
     2161 cacgttgttt ccggaaaata gattaatcgt cttctcctgt agcgttatcc cgatattgcg
     2221 gttttgctat taaaattttc gcacccattt accgtttatc ccttgtacat attccccggg
     2281 tttggcccgt tctacaagct tttgcccggc gagtttcgcg atatcatcca ccgacacatt
     2341 attctgcttt gccagttgtt gatagctcgc tttacgttcg gcattgatgt cggttactaa
     2401 tttctcagtt tcagcatctg ttttcagcgc aaccagataa ccataaaatg tttcgcctac
     2461 ccgcccctga gttctggctt catccagagt caatgccatt acattgctgc ttaccaagcc
     2521 aacaagaaat gcacaaagaa gtaatgttct tttcatcatc acctcaaaaa agatcgctac
     2581 gggtctcaag cagctcttcg acatctttgt ctgccttgat gatgatctca tgctcaattt
     2641 taacgttcat attgatagtg atcggttcct taggtgcagc gacttcaatg cgaggcgtac
     2701 agccaaccag cataaaagat gacgtcaacg cagccagtaa aattttcatt gtttatcctc
     2761 acactctttt ccttgcggac agtcatttcc cggtagatgc gcgttctgct ccagccatgc
     2821 ctggagatta tcgccaaagc gtaaactgcg ccacagcgta aaaatattct cttcatgatg
     2881 gtaattaaga tttaccgtac cgcttttacc atcaacgcga ctggtacctg taatattggc
     2941 ctgcatggtt aataaaccga gattatctaa attaattttt gtcgatgaac ggctaatttc
     3001 catatagcgc aaccagttaa ttgctgaacc cgcggtcata ttgtctttca ccaccgcatc
     3061 tgcggtgtct ttatccagcc gcaaggtcat tggcccgcta tttgccagcc agccgtcttt
     3121 cactatccat ttttcgttgt ttagccataa cggcaatgca ccactaaatg ccccggacat
     3181 ggcgaattgt ttcggattga cggcgctaac cagttcgctg gatgagagat tattcagccg
     3241 caacagcgcc gagtcatgtt gcggcatgcg taattgtttc atcagcacgt taccgcctaa
     3301 cacatcgacg ctaacatcag tcaacagcaa tggttcgtct tcactccacg gataacgccc
     3361 ttgcaaatca gccgtaatat ttttcgctgt caccagatta atcacttcgg caattcgcaa
     3421 cgtaacgggg ccgcgagtcc ccagatgcca ggctccatcg gcaaaacgga aaggcaggac
     3481 aaaatcgaca ccgttaacct gattatctgg catccaggca ctaccgcctt tcaacacacc
     3541 gtgccctccc gcgcggaatc cttgttcagg cgcagctgaa aatgcaacct gagcatacag
     3601 ttcgccatcg cgtaagttca tcttccagtc gggtggcacc agcggctgga atacagtcag
     3661 tgattgttta ggccaccagg cgttaccgcg cagacgaata ccgtcccagc gaccatttac
     3721 ccgaaccggg ccaatctcac cagcatgtaa atcgccttta aagaggaaat acgtcggatc
     3781 gcgcccatcg acgctaaatt ttaaggttga tggcggtaac acactgccgc cagtgaacag
     3841 tgtttgcccg gcgtccagtg acagcgcgcc gctgaaggag ggatgctgtg cgtcacgtac
     3901 ccagacgaca ggcttgtcga gaattaatcg cggcttttct accgtcatcg taccgtattg
     3961 taactggtcg aaaccggtgg agagatcggt cagcgtaatc gtgctgtcat gccactcacc
     4021 ctttcctgcg acatcccagg tggcattcat cggtgtaaaa ctgccttttc cccagtagcg
     4081 ccactgccag cggccagcgt cagggagaaa atcattcgcc agcccatcca tatgcagcac
     4141 gaaatcgccc agttcatttt catgcgcctg caaaatggcc tgcaaacgtc cgtcaacacc
     4201 acgttgggtg acttttacac ccgctaaagg ccagcggatt tcatcgatgt ccagcgaatc
     4261 gatgactctt cccttcgaac gtaataacgc gcctggctca aaggccagcg ttgggtcagt
     4321 cagacttccg cttagctgcg caggtaaacg ggcatataaa atgaggtccg cctgtttcgc
     4381 ttcaccggtc agttgcatag gcagctggct gttatccatg cttaattttc ctgggccaaa
     4441 atttagcacc gcgttgcctt tacccgcttg cccctgggtc agcacactca gtcgtccgct
     4501 gaccagagcg ttctcgagcc ctgcctgcca gttgtcgact ttgacaccca gtcggccact
     4561 caaaggaaaa cctgcatacg gccagctcca gcgaccatcg cttacggtca attgttgacg
     4621 agtaatttgc cacggcaaat cgagcaacgg atcgccgtta tcccgtgcca gcacaatcaa
     4681 ttgcccgcta ttttcctgcc agtccagctc ggcatccacc agtgacggtt cctgcggcaa
     4741 gtttaacgtc gcagtagcat ggccacttac aggaagtcca tccggcacta gcggcatagt
     4801 aaattccccc accagtttta ccggcggctg attttcaaac gcggcgacat ccagttcgct
     4861 gactgtaagt tgttgccctt taagctggcc ttgaaattta actttttcgc cctgataacg
     4921 cagttgctgg atatcagagg ttaacgcgag agagagtttt ccctgccact cctgccaggg
     4981 agaaaaaatc agtttatcga tattgatcca ggtgttaggc aacatggcct gccactgcgc
     5041 gagggttttt ggagcggcag gcgattgctc cgtctgcggc aatttcgcca gacaagcaga
     5101 atcaagttct accgtgccga cgttcaataa ccagcggctc ggatgtgaaa ggctggcgtt
     5161 ggtgatatgc gcaagctgac aatctcccac cagataacgg agatcgggaa tgattaaacc
     5221 tttacgcgta atgcgtgggc tttcatctaa tgcaatacgt gtcccgagcg gtagccagat
     5281 gcccgccagc gtgggaaccc acaagccgag cgtcatcagc agcgtcaacg gcacaagaat
     5341 aatcagtaat aacagcgcga gaacggcttt atatttaccc agcatgggta gttaatatcc
     5401 tgatttagcg aaaaattaag cattcaatac gggtattgtg gcatgtttaa ccgttcagtt
     5461 gaaggttgcg cctacactaa gcatagttgt tgatgaattt ttcaatatcg ccatagcttt
     5521 caattatatt tgaaattttg taaaatattt ttagtagctt aaatgtgatt caacatcact
     5581 ggagaaagtc ttatgaaact cgccgtttat agcacaaaac agtacgacaa gaagtacctg
     5641 caacaggtga acgagtcctt tggctttgag ctggaatttt ttgactttct gctgacggaa
     5701 aaaaccgcta aaactgccaa tggctgcgaa gcggtatgta ttttcgtaaa cgatgacggc
     5761 agccgcccgg tgctggaaga gctgaaaaag cacggcgtta aatatatcgc cctgcgctgt
     5821 gccggtttca ataacgtcga ccttgacgcg gcaaaagaac tggggctgaa agtagtccgt
     5881 gttccagcct atgatccaga agccgttgct gaacacgcca tcggtatgat gatgacgctg
     5941 aaccgtcgta ttcatcgcgc gtatcagcgt actcgtgacg ccaacttctc tctggaaggt
     6001 ctgaccggct ttactatgta tggaaaaacg gcaggcgtta tcggtaccgg taaaatcggt
     6061 gtggcgatgc tgcgcattct gaaaggtttt ggtatgcgtc tgctggcgtt cgatccgtat
     6121 ccaagtgcgg cggcactgga actcggtgtg gagtatgtcg atctgccaac cctgttctct
     6181 gaatcagacg ttatctctct gcactgcccg ctgacaccgg aaaactacca tctgttgaac
     6241 gaagccgcct tcgatcagat gaaaaatggc gtgatgatcg tcaataccag tcgcggtgca
     6301 ttgattgatt ctcaggcggc aattgaagcg ctgaaaaatc agaaaattgg ttcgttgggt
     6361 atggacgtgt atgagaacga acgcgatctg ttctttgaag ataaatccaa cgacgtaatt
     6421 caggatgacg tattccgtcg cctgtctgcc tgccacaacg tgctgtttac cgggcatcag
     6481 gcattcctga cagcagaagc gctgaccagt atttctcaga ctacgctgca aaacttaagc
     6541 aatctggaaa aaggcgaaac ctgcccgaac gaactggttt aatcttgccg ctcccctgca
     6601 ttccagggga gcttattcag ataatcccca aagacctttc atcctctatt cttaaaatag
     6661 tcctgaatca gaaacagtaa ttgagaacca caatgaagaa agtagccgcg cttgttgcgc
     6721 taagcctgct gatggcggga tgtgtaagta gtgacaaaat tgctgtaacg ccagaacagc
     6781 tacagcatca tcgctttgtg ctggaaagcg taaacggtaa gcccgtgacc agcgataaaa
     6841 atccgccaga aatcagcttt ggtgaaaaga tgatgatttc cggcagcatg tgtaaccgct
     6901 ttagcggtga aggcaaactg tctaatggtg aactgaccgc caaagggctg gcaatgaccc
     6961 gtatgatgtg cgctaacccg cagcttaatg aactcgataa caccattagc gaaatgctga
     7021 aagaaggtgc acaagtggat ctgaccgcga accagttaac gctggcgacc gcgaaacaga
     7081 cattaactta taagctggcg gatttaatga attaatagct gccacagctc ccggcggcaa
     7141 gtgactgttc gctacagcgt ttgccgttgg gtaatgcaca catcccaatc gccgtaccat
     7201 ccagttggcg ggcaacagaa agcgaaccgc cgatcattgc acaatttgct tctccactac
     7261 tggacatcga cgcttttaaa cctggcgcta cgtgcgccgc agtcgcctgc tgaacaggtt
     7321 cactactaca cgccgacaac aataaagcgg cacaccctac ccaaaacgct gctcgcatct
     7381 tttcttcctc tgatcttcaa gccaaacgac accgccataa ataataggca gcacagaggg
     7441 cggcgtcgag agctgtcctg cgcgttgccc cgcattttta cttttttatg gctatttttt
     7501 tgccctctgt ttgatcaaaa cattcattac gctaatgtgg gggacacaaa agcgaaaatg
     7561 cagaagaaag ccatttgcta aaattgaacg attactactg ggcgcgcagc aatttcgtgc
     7621 gcccctcatt tgcgcaatgt aagggtgtca tatgattact attgacggta atggcgcggt
     7681 tgcttcggtg gcgtttcgca ccagtgaagt tatcgccatc taccctatta cccccagttc
     7741 cacgatggca gaacaggctg atgcatgggc cggaaacggc ttgaaaaacg tttggggaga
     7801 cacaccacgc gtggttgaaa tgcagtcgga agcgggtgct atcgctaccg tgcatggtgc
     7861 tttgcaaact ggtgccctct caacatcgtt tacgtcatcg cagggtttgc tgctgatgat
     7921 cccaacgctg tacaaactgg caggcgaact gacgccgttt gtcctgcatg ttgcggcacg
     7981 taccgttgcc acacatgcac tctctatttt tggcgatcat tccgacgtta tggcggtgcg
     8041 ccagacgggt tgcgcgatgt tgtgtgcagc aagcgtccag gaagcgcaag actttgctct
     8101 catttcgcac atcgcgacgc tgaaaagccg cgtgccattt attcatttct ttgatggttt
     8161 ccgcacatct cacgaaatca ataaaattgt cccgctggcc gatgacacga ttcttgaact
     8221 gatgccgcag gcagaaattg atgctcatcg cgcccgggca ctcaacccgg aacatccggt
     8281 gatccgcggt acgtccgcca atcctgacac ttatttccag tctcgcgaag ccaccaaccc
     8341 atggtacaac gcagtctatg accatgttga acaggcgatg aatgatttcg ctgccgcgac
     8401 aggtcgtcag tatcagccgt ttgagtatta cgggcatccg caagcggaac gggtgattat
     8461 cctgatgggc tcagccattg gcacatgtga agaagtggtt gatgaattgc tgacccgtgg
     8521 cgaaaaagtc ggcgtgctga aagttcgcct gtaccgcccc ttctccgcca aacacttgct
     8581 gcaagctctg ccgggatccg tgcgcaacgt ggcggtactg gacagaacca aagagtctgg
     8641 tgcccaggca gaaccgctct atctggatgt gatgaccgca ctggcagaag cctttaataa
     8701 tggcgagcgt gaaaccctgc cccgtgtcat tggtgggcgc tatggtcttt catccaaaga
     8761 atttggcccg gactgtgtac tggcggtatt tgccgagctc aacgcggcta aaccgaaagc
     8821 gcgctttacg gttggtattt acgatgatgt aaccaatctg tcactgccgt tgccggaaaa
     8881 caccctacca aactcggcga aactggaagc cttgttttat ggccttggta gtgatggcag
     8941 cgtttccgcg accaaaaaca atatcaagat tatcggaaat tccacgccgt ggtacgcaca
     9001 gggctatttt gtttacgact ccaaaaaggc gggcggcctg acggtttctc accttcgcgt
     9061 gagcgaacag ccgattcgtt ccgcttatct catttcccag gctgattttg ttggctgcca
     9121 ccagttgcag tttatcgata aatatcagat ggctgagcgt ttaaaacctg gcggcatttt
     9181 cctgcttaac acgccgtaca gcgcagctga agtgtggtcg cgcttgccgc aggaagttca
     9241 ggccgtgcta aaccagaaaa aagcgcgctt ctatgtgatt aacgcggcga aaatcgcccg
     9301 cgaatgtggc ctggcggccc gtattaatac cgtcatgcag atggcttttt tccatctgac
     9361 gcagattctg cctggcgata gcgccctggc agaattgcag ggtgcgattg ccaaaagtta
     9421 cagtagcaaa ggccaggatc tggtggaacg caactggcag gctctggcgc tggcacgtga
     9481 atccgtagaa gaagttccgt tgcagccagt taatccgcat agcgccaatc gaccgccagt
     9541 ggtttccgat gccgctcctg atttcgtgaa aaccgtaacc gctgcgatgc tcgccgggct
     9601 tggtgatgcc ctccccgttt cggcgctgcc gccagacggc acctggccga tgggcaccac
     9661 gcgctgggaa aaacgcaata tcgccgaaga gatccccatc tggaaagagg aactctgtac
     9721 ccaatgtaac cactgcgtcg ctgcttgccc acactcagct attcgcgcaa aagtggtacc
     9781 gcctgaagcg atggaaaacg cccctgccag cctgcattcg ctggatgtga aatcgcgtga
     9841 tatgcgcggg cagaaatatg tcttgcaggt ggctccggaa gattgcaccg gttgtaacct
     9901 gtgcgtcgaa gtttgcccgg cgaaagaccg tcagaatcca gagattaaag ccatcaatat
     9961 gatgtctcgc ctggaacatg tcgaagaaga gaaaatcaat tacgatttct tcctcaacct
    10021 cccagaaatc gaccgtagca aactggaacg tattgatatt cgtacatcgc agctgattac
    10081 accgctgttt gaatattcag gtgcttgctc cggttgtggc gagacgccgt atattaaatt
    10141 gctgactcag ctctatggtg accggatgtt gatcgctaac gccaccggct gttcttcaat
    10201 ttatggcggt aacctgccct ctacaccgta taccaccgat gccaacggtc gtggaccggc
    10261 atgggcgaac tcgctatttg aagataacgc cgaatttggc cttggtttcc gcctgacggt
    10321 cgatcaacac cgtgtccgcg tgctgcgtct gctggatcaa tttgccgata aaatcccggc
    10381 ggaattacta actgcgttga aatcagacgc caagccagag gttcgtcgtg aacaggttgc
    10441 agctttacgc cagcaactca aagatgttgc cgaagcacat gaactgctac gtgacgcaga
    10501 tgcactggta gaaaaatcaa tctggctgat tggtggtgat ggctgggctt acgatatcgg
    10561 ctttggcggt ctggatcatg tattgagttt gacggaaaac gtcaacattc tggtgctgga
    10621 tacgcaatgc tattccaaca ccggtggtca ggcgtcgaaa gcgacaccgc tgggtgcagt
    10681 aactaagttc ggcgagcacg gcaaacgtaa agcgcgtaaa gatcttggcg tcagcatgat
    10741 gatgtacggt catgtttatg tggcgcagat ttctctcggc gcgcagctca accagacggt
    10801 gaaagcgatt caggaagcgg aagcgtatcc ggggccatcg ctgatcatcg cttatagccc
    10861 ctgtgaagag catggttacg atctggcact cagtcacgac cagatgcgcc aactcactgc
    10921 caccggcttc tggccgctat atcgcttcga tccacgtcga gccgatgaag gcaaactgcc
    10981 gctggcactg gattcacgcc cgccgtcaga agcactggaa gaaacgttac ttcacgagca
    11041 gcgtttccgt cgtctgaatt cgcagcagcc agaagtggca gaacagttat ggaaagatgc
    11101 tgcagctgat ttgcaaaaac gctatgactt cctggcacaa atggccggaa aagcggaaaa
    11161 aagcaacacc gattaaatgc actggataag gattatccaa ttctaaaaaa aagcccggac
    11221 gactgttcgg gcttgtcttt ttatatgttg gaaaatcagt agcaatgcaa tggcccaaca
    11281 gaaaataaaa tctgtgacaa taaaggcata taacccgcgc agaataacgt atagcaaatt
    11341 aaaagtataa tttttatttt gtatgtatta tcttcaatac attcaccaga cttattatca
    11401 tttccgtaga attatttgat taaggtttta cttaaggcgt aacaaatgat atttatcggc
    11461 taactgaact tctcctttag gatgttttca ctccccgaaa tggggataaa aggcaaataa
    11521 aataactaaa ggatttattc aatgaaaagc aaagtactgg cacttttaat tcctgccctg
    11581 ctcgccgcag gtgctgcaca tgcagccgaa gtttataata aagacggcaa caaattagat
    11641 ctgtatggca aagttgatgg cctgcattat ttttctgata attcagcgaa agatggcgac
    11701 cagagctatg cgcgtctggg ttttaaaggc gaaacccaaa ttaacgatca actcactggc
    11761 tacggtcaat gggaatacaa tattcaggca aacaacactg aatcttcaac aaaccagtca
    11821 tggacccgtc tggcatttgc cggactgaaa tttgcagatt acggttcttt cgattacgga
    11881 cgtaattatg gcgtaatgta cgacatcgaa ggctggaccg atatgctgcc tgaatttggc
    11941 ggtgactctt ataccaatgc agacaacttt atgactggtc gtgccaacgg cgtcgcgact
    12001 tatcgtaata ctgatttctt cggtctggta aatggtctga acttcgcggt gcagtatcaa
    12061 ggtaacaacg aaggagccag taatggtcag gaaggcacca acaacggacg tgatgttcgc
    12121 catgaaaacg gtgacggctg gggtctttcc acaacatatg atttaggcat gggctttagc
    12181 gctggtgcgg catacacctc ttctgaccgc accaatgacc aggttaacca tactgcggcg
    12241 ggtggtgata aagcagacgc gtggactgct gggctaaaat acgatgctaa caatatttac
    12301 ctggcaacca tgtattcaga aacgcgtaac atgaccccgt ttggcgacag cgattatgca
    12361 gttgcgaaca aaacccagaa ctttgaagtt actgcacagt accagtttga ttttggtctg
    12421 cgtccggcag tctctttcct gatgtctaaa ggccgtgacc tgcacgctgc cggtggtgca
    12481 gacaacccgg caggtgttga tgataaagat ctggttaaat acgccgatgt tggcgcgact
    12541 tactatttca ataaaaacat gtccacttat gttgactata aaatcaacct gttggatgaa
    12601 gatgacagct tctacgctgc caacggcatc tctaccgatg atattgtcgc tttaggtctg
    12661 gtttatcagt tctaaatcct cctgcccgct gttatggcgg gctttttctg cttattcttc
    12721 ctcctttgat ctaaattaaa aatgtgaact ccgtcattac acaaaaagtg tcatctggcg
    12781 ttacacttta tgcggatact aaaacaggag gttttatgaa cagaacgatt cttgtcccta
    12841 tcgatatttc cgattcagaa ttaactcaac gcgtgattag ccacgttgag gcagaggcaa
    12901 agattgatga tgcagaggtt catttcctga cggtaatacc ttcactgccc tactatgcct
    12961 ctctgggttt agcgtattcc gcagaattac cggcaatgga tgacctgaaa gcggaagcca
    13021 aatcgcaact ggaagagatc attagaaaat ttaaactgcc aaccgacaga gtgcatgtcc
    13081 atgttgagga aggttcgccc aaagaccgca ttctggaatt ggcgaagaag atccccgctc
    13141 atatgatcat cattgcttcc catcgaccgg atatcaccac ttatctgctc ggttccaacg
    13201 ccgcagctgt agtgcgtcat gcagagtgct ccgtgctggt tgtgcgctga cactaacgcc
    13261 cgcacattgc tgcgggcttt ttgattcatt tcgcaaatgt gctgacattt tcccctctaa
    13321 tccgtaccat acacgccaca gtttttatat cagatttctt atgctgggcg ttccggcatg
    13381 acgcatactc ttctgatgcc atataacgaa ttgagtcgct tttaaatgtc gcaaaatcaa
    13441 gaaattagta agaaagaaca atacaacctg aacaagtaag ggcaaaaatc acaactatct
    13501 gacccataag tattttattt atttttcaat atgttaaaca ttatcggcaa ctacaataag
    13561 ccacgataac ccactctttt tttgccccat ttttgcccct tttgcagtgt tttgccccat
    13621 ttttgccacc gaaaatgcga gaatttctca atctgtagcc tgcaaattgc gcccatgagt
    13681 tctttccaag agaatctgtt tcggctggaa atcattcaat actcacgcta tcgaaagtta
    13741 tccagcctgt tgtggaacgt ccttgcatgt gacgtatcgc gggtctcttt acgattttta
    13801 tctgtattgg tgaattataa gcaatctgaa tctatgcatg acgtgtggtg cctgttttgc
    13861 ttttttccat ctatttttgc tgggtcgaag c
//
LOCUS       DAPHCV010000117        13832 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.117, whole genome shotgun sequence.
ACCESSION   DAPHCV010000117 DAPHCV010000000
VERSION     DAPHCV010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.117"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..881)
                     /gene="yhaJ"
                     /locus_tag="RH033_003783"
     CDS             complement(<1..881)
                     /gene="yhaJ"
                     /locus_tag="RH033_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HDV2992906.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKL"
     gene            986..1687
                     /locus_tag="RH033_003784"
     CDS             986..1687
                     /locus_tag="RH033_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HDV2992907.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGETLLLSTQPGISYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTVGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1710..1874
                     /locus_tag="RH033_003785"
     CDS             1710..1874
                     /locus_tag="RH033_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992908.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2006..3315)
                     /locus_tag="RH033_003786"
                     /pseudo
     CDS             complement(2006..3315)
                     /locus_tag="RH033_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026202.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
     gene            complement(3343..4674)
                     /locus_tag="RH033_003787"
     CDS             complement(3343..4674)
                     /locus_tag="RH033_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDV2992909.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGVLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(4949..6313)
                     /gene="tdcG"
                     /locus_tag="RH033_003788"
     CDS             complement(4949..6313)
                     /gene="tdcG"
                     /locus_tag="RH033_003788"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDV2992910.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETPVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6385..6774)
                     /locus_tag="RH033_003789"
     CDS             complement(6385..6774)
                     /locus_tag="RH033_003789"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HDV2992911.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(6788..9082)
                     /gene="tdcE"
                     /locus_tag="RH033_003790"
     CDS             complement(6788..9082)
                     /gene="tdcE"
                     /locus_tag="RH033_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HDV2992912.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPLDFDTNIATTITAHDAGYIDQPLEKIV
                     GLQTDAPLKRALHPFGGINMIRSSFHAYGREMDSEFEYIFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRRALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEALPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGMDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9116..10324)
                     /gene="tdcD"
                     /locus_tag="RH033_003791"
     CDS             complement(9116..10324)
                     /gene="tdcD"
                     /locus_tag="RH033_003791"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HDV2992913.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLNASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLHRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10350..11681)
                     /gene="tdcC"
                     /locus_tag="RH033_003792"
     CDS             complement(10350..11681)
                     /gene="tdcC"
                     /locus_tag="RH033_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HDV2992914.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11703..12692)
                     /gene="tdcB"
                     /locus_tag="RH033_003793"
     CDS             complement(11703..12692)
                     /gene="tdcB"
                     /locus_tag="RH033_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HDV2992915.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(12791..13729)
                     /gene="tdcA"
                     /locus_tag="RH033_003794"
     CDS             complement(12791..13729)
                     /gene="tdcA"
                     /locus_tag="RH033_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HDV2992916.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVD"
BASE COUNT         3591 a         3739 c         3240 g         3262 t
ORIGIN      
        1 agtttgggaa tttcccgcag acaccaggat ttcgcctccc ccatactgtc acgacgccag
       61 gccataataa tatcgatctc gctggtcgat tccgggctga cgacacgcaa ccgcccttcc
      121 gcgatgtctt tttcgaccat cggatacggc atcgtcgcca cgccaagccc cgccagtaac
      181 gcctgacgtt tatcttcaat cgtgctcacc gttaaacgcg gctgtttgtc cagtagctgt
      241 acggtcaata ccgggcgctc acgagcggta tccgccaccg caattccacg atatttcacg
      301 cgcgtcactt cagataacgg ttccggctcc tgatgaatcg ggtgatcagg cgcggcaacg
      361 tagacgttca ttaacgtata gagcttgcgc gagttgatct ccgacgagga gcgaaaatgc
      421 atatccggcg cgataacaat atccgcccgc ccctgctcca gccgttccca tgcccccgcc
      481 agcacttcgg tgatgattgc cagttgggta ttcgcttttg ccgccagttt gtcaattaac
      541 gggaaaaagg caggtgtcgg taccagcgcc tcggtcacaa tggtgagatg cgtttcccaa
      601 ccgcgcgcga gagcttccgc atcggtagtc agtttatctg cggcttccag caaaacgcgt
      661 cctcgctcca gcaacatcct cccgacattg gtgaatttgg tgcgatggcc cgagcggtca
      721 aacaacacca catccagctc ttcttccagt ttttgcatgg tatagctaag tgcggaaggc
      781 acgcgtccca gctcatccgc cgccgccgca aaactgcccc ggcgatcgat cgcatccata
      841 acccgtagtg cttccagcgt taatgccctt tctttggcca tttcgttctc attcaggaaa
      901 tttgaacata ccgggcagaa tatctggcta acaatgtagc gtccagcccc ttaccataaa
      961 aggaagtaaa gagaggtcaa gaattatgat tactacccga actgccaggc agtgtggaca
     1021 agcagactac ggatggttgc aggcccggta tactttttcc tttggacact acttcgaccc
     1081 gaaattgtta ggctatgcct ccctgcgcgt gcttaaccag gaagtgctgg ccccaggtgc
     1141 cgcctttcag ccgcgaacct atcccaaagt cgatatttta aatgtgattc tggatggaga
     1201 agctgagtat cgcgacagtg aaggcaatca tgttcaggcc agcgccggag agacattgct
     1261 gctctctacc cagccgggta tcagctatag cgaacacaat ctcagcaaag acaaaccgtt
     1321 aacgcgaatg cagctttggc tggatgcctg cccgcagcga gagaatccgc tgattcaaaa
     1381 actggcgctt aatatgggca agcagcaatt aatcgcctcg ccagagggca cagtgggtag
     1441 cctgcaactg cgccagcaag tgtggctgca ccatatcgtg ctcgacaaag gcgaaagtgc
     1501 gaatttccag ttgcatggac cacgcgcgta tttgcaatcg attcacggga aatttcatgc
     1561 gcttacgcat catgaagaga aagcggcgct aacctgcggt gatggggcgt ttattcgtga
     1621 cgaggctaac attacgctgg ttgccgattc cccactgcgc gctttgctga tagatttgcc
     1681 tgtctagttg tttttacagg gagatgatga tgagtaaaaa atcggccaaa aagcgccagc
     1741 cagtgaagcc cgtggtggcg aaagaacctg ctcgtaccgc caaaaatttt ggctatgaag
     1801 agatgttgag cgaactggaa gccatcgtcg cggatgctga aacgcgttta gccgaggatg
     1861 aagctaccgc gtaaagacag gtgccggatg cggcgtaaac gccttatccg gcctacaaaa
     1921 cccaacaaca tcaacaaatt gtgcaattcg taggccagat aagacgcgcc agcatcgcat
     1981 ccggcacgat acccaaaact ggcgtttatc tggccttgct cgccataatc tcgataatct
     2041 gccgatccgt ttgctgcatc gaatggcttg ctaacgcaca caggttggca atcgactgct
     2101 caacatcatg cgccacaatc ccttcattac cggttacggc ggtatcatcc agcgccatta
     2161 acaccgcttt ccacgcagcc gaagcactgg tcgaaacctt catcgcgcag ctgttcgatg
     2221 caccatcgca aatcatgcca ctgacatcgc cgatcatact gctgatcgcc atcgaaatgg
     2281 tttcataacg cccatccacc agccatgcca tcccggcggc ggcccccatt gctgcggtcg
     2341 ttgcggcaca cagcgcagac aaacgcggta actggttatg gatgtaaatt gcgctcaaat
     2401 gcgaaagcat cagcgcacgc gccagccgtt catcatcggc cccgaagtgt tctgctacca
     2461 ccaccactgg cattgttgca gtgatcccct ggttacccga gccggagtta ctcattgccg
     2521 gaagcgtagc gccgcccata cgcgcatcgg atgccgcgct ggtacgaatc acaatggatg
     2581 aagagagatc tttcgccagc aaaccgcgtg cacactgttt ttccagcgtc gcgccaatat
     2641 gcagccccca cttaccgctc aaaccttcct gcgataacgc acaatttagc ttcgcggaat
     2701 cgagaataaa gcggatcgcc gcaaacggga cttcattgac gaacttcagg atctcagcca
     2761 gcgtcgttct tgaaagcacc gtcagcggcg actcttgctc gccctctgtc acacacgcct
     2821 gctgggtaaa caccacacca ttgtgcgtct caatatgcac aatgttggta tgcccgccga
     2881 cgatggtgac acacgcccac ttctcaccgt tccagacttt ggcgcgtgag aagaggattt
     2941 catcgcaagg ttcctggatc ttaactgaga ctttccccgc cgccagcagt gctttggcat
     3001 cggcaattgc ctgcgcagtt gcgtctttca gcacttccag cccggcgtta gcatttccac
     3061 ctaacgctcc cagcgccgcc gcaattggca gccccaccat tcccgtgccg ggaacggtga
     3121 cgcccagacc gttcttcatc agatttggcg aaacccaggc ttctacacgt tcaaccggac
     3181 cttccagttc tgccgcagca accgccgccg ccagcgccag tgaaatcggt tcagtacatc
     3241 ccagcgccgg ttttacttcc tcctgaacgg cgaggatgta acgctgccaa acggatttaa
     3301 agtcgaatca aacatattaa aaaccttaaa atttcaggta aatcaagaaa atgccaggaa
     3361 cggagaaaca caaagcaaca aaccagtgac gataatcagg tacagagaca tccctttgta
     3421 tttgtgcaat gccggtactt tgtaaaccag ccacgccggg atcaggcacc ctaccatgcc
     3481 gaaaatcggg ctacagatag aggtgaagct caacaccggt gcgttcagta cgatggcgct
     3541 ccaggccagc aaaatggcga aaatcatgat gccgcgctga acgagatttt cgttaatctt
     3601 ctcggcaggc atcttgcgac gcaggatgtt cattacgatc ccttgcgttg cttcgcgaaa
     3661 gcctaaatag acgccaaaga atgcagtcat tacagcaaag atattgagaa tgacgctgac
     3721 cactttcacc cacgctgcgc catcaccgct aataaactgt gcggcaatcg ccagcgcgga
     3781 aatattctgc tcgtaggctt ttaccgcttc gtcatgtccc atcgccagcg tgaacgacac
     3841 ggcgtagaaa aagacggtga caaacaaaat accaaacgcg atattcattg cccgcaatgc
     3901 tttatgccgc gccacttcaa ttgatttttc ccgcgagcga taagagatca ccatcgggct
     3961 taacgtctgg ataaacagaa tcgacgtcag ggtaaacggc agcgtaataa tggcgttttt
     4021 caccagcagc cccagcggcg gtagcgaacc gacgttatac agatgccaca ttcccaccat
     4081 cgacacgccc agcgccgcga ccaccagcag cttggtcagc accatgccgg tcgaaatttt
     4141 aaataacaat ttctcgccgc gtgaggagat cgccaccaga atgcaaatca gtaccagacc
     4201 ataaaaggga ctgtctgaca gcaccccttc cgtcacgccg aaggtatgca ggtaggaagc
     4261 actatcgttg gtgatggcgg tggaatagac gaacatccaa atcaccagca ttacgaaata
     4321 gagcgcacct aacaggatgc cccagttttt acctaaataa ccgctgatga cgctcgggta
     4381 atctttacac tccggtgatt ctgccagcgt attaataaac aaccgctgaa acagatacat
     4441 tgccgggtaa ccaatcaccg atgagagcaa aaatacccac aatcccatca aaccgacctg
     4501 caccgggaga aaaacaatcc ccgcgccaat cgccatcccg atactcataa tcacccagcc
     4561 ggtgtcagta ctgtcgaatt tgatcgcttc tcgccactcg ctctcactca ttccagcacg
     4621 acccgccggg gtcgaagcgt ctgcaatgac gcctttattc gatgcaattt ccataatttc
     4681 tcgctcaata ttttgtaggg cttattattt ttttccgagc cgcatcaagg cgatatgcgg
     4741 tctacgtttt tgcaggcgag ttattagaat aaagaaatga tacgcgcgag gtcagagaaa
     4801 atcttcacaa tcaagacttt gaaataacgg agatggataa gaattttcta ctaaattaat
     4861 cgcaagagaa tattcattct ctatttgcgg cagatcacaa aaaaaaggtg cacatttgtg
     4921 cacccaagga tgaaagctga cagcaatgtc agccacagac cactttaatg gccagtcctc
     4981 cgcgtgatgt ttcgcggtat ttatcgttca tatctttgcc ggtttcatac atcgtctcga
     5041 tcactttatc gagtgaaaca cgcggtgccg aggtgcggcg cattgccatc cgcgcggcgt
     5101 ttactgcttt cacggcatta atggcattac gttcaatgca cgggatttgt acctgtccgg
     5161 caaccggatc gcaggtcagc ccaaggttat gctccatcgc gatttccgcc gcattgcata
     5221 cctgcgccgg actgccgccc aggagttcag ttaaccctgc cgccgccatt gaacaggcca
     5281 cgccaatctc cccctgacag ccgacctccg caccagagat ggaggcgttc attttataca
     5341 gcgcgccaat agccccggct gcaagaaaat agcgggcaat tgaccgctcg tttaccggac
     5401 gacggaactt atcgtaataa gccagcactg ccggaataat gccgcacgca ccgttagtcg
     5461 gtgccgttac cacgcgcccg ccagctgcgt tttcttcact aaccgccagc gcgtacatat
     5521 tgatccagtc gatgacattc atcggatcgt tagagatgtt atcgctggaa accagctgac
     5581 gacgcagcgc tacggcgcgg cgcggcacat tgagcggacc aggcagcacg ccttcagtgt
     5641 tcatcccgcg ttcaataccg tcatgcatca cttgccagat acgggcaaac cctgcgtcaa
     5701 tttccgcttt gctgcgcagc gccagctcgt tgtgcatcat caggccagat atagacaggc
     5761 cgttgtaatc acacattttc agcagttcac ctgctgagtg gaaatcgtaa ggtacgggtg
     5821 tttcgacatc gtgcgacagg ccgaagtgtt cttcttcgac aataaacccg ccgccgacag
     5881 agtaataggt tttgcttaat agcgcttcct gtcccttcca ggcagtgatc cgcattccgt
     5941 tctcatggcg aggcaacatt tcgggatgga agataatgtt ctttgctaca ggaaaatcaa
     6001 caatatgcgc gccagaagcc accggcaacc gcccgctgcg cgtcactaac tctataaatg
     6061 cagggatctc atcaatgaca acatcctgcg gactgtttcc tgccagtccc atgatgatgg
     6121 cgacatccgt ggcatggcct ttgcccgtca gtgacaacga cccgtacaga tcgaccacaa
     6181 tatggctcgt cgcggttaat aagccgctac tttccagccg atcaataaaa ctttttccgg
     6241 cattcattgg ccccacagta tgcgaactgg agggaccaat cccaattttg aaaatatcga
     6301 atgcactaat catatccaca ccctcggatt gccgttcagt aaagtggagc ggaacgacct
     6361 tgcgaccgtc ccgctcacga ggccttacgc actacgtact gcgatggctt caatttccaa
     6421 cttcacatct ttcggcaaac gcgcgacctg cacacagctc cgggtcgggt aggtcgcctg
     6481 atgttcatcg aagaactgct tatagacttc attgatggtg gcaaaatcat tcagatcggt
     6541 gatgaacacg gtcatcttga tgatatcgcc cacactcagc ccggcagcaa ccacgatcgc
     6601 tttgacgttt tcgaggctta aacgcgcctg atcttgcaca tcagccggga tctcaccggt
     6661 ctgtgggcaa accggaattt gcccggaggt gaagaccatg ctgcctaaat caacgccctg
     6721 aacataaggg ccgattgcgc ctggggcacg ttgcgtttcg ataatctttt tcatacatcc
     6781 tccggcgtca gagcgcctgg gtaaaggtac gtgaaataac atcctgttgc tgttcacggg
     6841 tcagtgcgtt aaagcgcacg gcgtagccag agacacggat tgtcaggtta ggatattttt
     6901 ccgggtgctc gatggcatcc agcagcattt cccgattcat tacgttgacg ttgaggtgtt
     6961 gaccgccttc gacatccgct tcgtggtgga aatacccatc cagcaggccg acaaggttgg
     7021 ttttacgtac tggatcttct ttgcccagcg ccgcagggac gatggagaag gtgtacgaga
     7081 tcccatcttt ggcgtaggtg aacggcagtt tcgccaccga cgtcaatgag gccacggcgc
     7141 ctttgcgatc acgaccgtgc atcgggttag cgcccggcgc gaacggtgtt ccggcgcggc
     7201 gaccgtccgg cgtgttcccg gttttctgac cgtacaccac gttagaggtg atagtcagaa
     7261 tcgattgggt cggcacagca ttgcgatagg ttggcaaagc tttaattttc ttcataaagc
     7321 gttcaaccag atcgcaggca atgctgtcta cgcgctcgtc gttgttgcca tactgcggat
     7381 attcgccgtc gatttcgaaa tccaccgcca ggccgttttc gtcacggatt ggtttcacac
     7441 gagcatattt gatggcagac agggagtccg ttgccaccga caggcccgcg atgccgcacg
     7501 ccatggtgcg atagacatca cgatcgtgta gcgccatcag cgaggcttca tagctgtact
     7561 tgtcgtgcat gtagtggatg atattcagcg cgctgatgta ctgtaccgcc agccagtcca
     7621 tgaagtggtc gaggctatcc atcactttgt cgtagtccag cacgtcatcc atcaacggag
     7681 ccgttttcgg gccaacctga atcttcagct tctcgtccat tccgccgttg attgcgtaca
     7741 gtagcgtttt cgccaggtta gcgcgcgcac cgaagaactg catctgctta ccaatcacca
     7801 ttgggctgac gcaacaggca atcgcgtaat cgtcgctatt gaagtcagta cgcatcagat
     7861 cgtcgttttc atactgcaaa gaggatgtga cgatagacac ctgcgcggca tattttttga
     7921 aggcaatcgg caaggcttcc gaccacagaa tagtcaggtt aggttccggt gcaggcccca
     7981 tagtgtgcag ggtgtgtaaa tagcggaagg agtttttggt caccagggtt cgaccgtcca
     8041 gccccatccc gccgatcact tccgtcgccc agatcgggtc gccggagaac agcgaatcaa
     8101 attccggcgt acgcaagaag cgcaccatac ggatcttcat gatgaagtgg tcgatcagtt
     8161 cctgtgcctg ctgctcattg agtacgcctg ctttaaagtc gcgctcaatg tagatatcga
     8221 ggaacgatgc cgtgcgcccc agcgacatcg cgccgccatt ttgcgatttc actgccgcca
     8281 ggtaagcgaa gtagagccac tgcaccgctt cctgtgcatt ctgcgccgga cgggagatgt
     8341 caaaaccgta tttcgccgcc atttcttgaa tctgcaacag cgcacgacga tgttccgcca
     8401 gctcctcacg cagacggatc gtagcttcga gatcttcgcc tttttccaga cgggactgga
     8461 gatcggcaaa ctgcagttcg cgttcacgta ccagataact gatgccgtaa agcgccacgc
     8521 ggcggtagtc accgatgata cgcccacgac cataaccatc tggtaaaccg gtcagtacac
     8581 cagatttacg gcagcgcagc atatccggtg agtaaacatc aaatacgccc tggttatggg
     8641 ttttacgcag atcggtaaag atgtattcaa attcgctatc catttcacga ccatacgcgt
     8701 ggaatgaact tctaatcata ttaatgccgc cgaacggatg cagcgcacgc tttaatggcg
     8761 catccgtttg cagacccact attttttcca gcggctggtc aatatatcct gcatcatgag
     8821 cggtaattgt ggtggcaata ttggtatcga aatccaatgg cgcgtgagtc gcattttcga
     8881 tacggatgcc ttccatcact ttttcccaca attccgtggt ggcaggcgtc gcttcggcga
     8941 gaaaagattc atcaccttca tacggtgtat agttatgttg aataaaatcg cggacattaa
     9001 tttcgttttt ccagtccgta cctttaaagc caagccatgc gtcggcgtac agcttatcgc
     9061 tggtatcaat atctaccttc atgaaaaata atctctctac aatacttcaa ctaaattatg
     9121 caaattctgc gggcgcgttc actttgccta aatgaatggc atccaaagca atcatttttt
     9181 cttcgttagt cggaattacg gcgcaaatga cacgcgcatt ttcactggaa acaattcgct
     9241 ctccaaaaga gttagagcga ttattcattt ctgtatcaat ctctacgcct aatacagcta
     9301 aatgttccat gaccagacga cgaattaaac ttgaattctc tcctattccg ccggtgaata
     9361 taattccatc caggcgatgt aatgaagcag cgtgtccggc aatatgacgg gcaattcgat
     9421 gaacaaaggt tttaattgcc aattgcgcgc gttcgtgacc ttcatgccag gctttttcca
     9481 gaacacgtaa atccgaagaa aggccggaaa tacctaataa tcccgactct ttattcacga
     9541 cgcgttccag gtcacccagg ctctggttgg tttggctggc gacccaggac atcgccccga
     9601 agtcgacatc accactgcgg gtacccatca tcaagccttc cagcggcgtc attcccattg
     9661 aggtatcaac actctgaccg ttgcgaactg cgcagattga cgcgccattg ccaagatgcg
     9721 ccacaaccag gccggagtca tcttccgcca gattcagcaa agaatgcgcg cgctgggaaa
     9781 catagcggtg cgacgtgccg tggaaaccat aacggcgtac acctaactct tcataatatt
     9841 tccacggcag gccgtataaa taagcttccg gagccatcgt ctggtggaaa ctggtatcaa
     9901 ataccgctac ctgagttacg cccggaaata attgctgcgc cgattcaata ccacttaaat
     9961 tggcgtaatt atgcaggggt gccagtggag aaacgcgacg gatattatca atgacttcat
    10021 cggtaataat ggcggactcg gtaaaaatac tgccgccgtg agcgatgcgg tggccaatta
    10081 aggccacact gtcatttaaa ttccgttttt ccagttcaaa tgcaattgcc ttcaatgcac
    10141 cttcgtagct gtggtgagcc agcggtgctg gctctccccc atttacggat aagaatgcat
    10201 tttccgagtt aataccgtcg gcaatacctg acattaatac ttcacagtcg ctggcattga
    10261 gtacggaaaa cttaatcgaa gacgaaccac agttaataac caaaacaacc ggaaattcat
    10321 tcatctcttt tctcatcctg agttacggat taaaacagtt tgtatacgat gttcaggata
    10381 gtcagcagac caatcacggt aacaaacacg ttatccagac gaccacggta tttcgccaga
    10441 gacggcgctt tacgaatggc atacatcggc aacaggcata gcagtgatgc gataatcggt
    10501 gcgcccatgg cttcaatcag gtcgaggatg ttcgggttgg cgtaggcaac aacccaggtg
    10561 gagcccatga tgaagatcat gctgagagta ttcagtttac ccagcgacac tttggttttg
    10621 tcacctttat aaccgaactt cagaatcagg ccattcaagc cttccagcgt ccccagatag
    10681 tgaccgaaga aagatttgaa gatagccacg agtgcgatga tggaagccgc atattccagt
    10741 gtaatcgcga acgttgtttt ggtaccggtc atggacgcaa agtggttagc cagataagaa
    10801 agcactggaa tattctgcgc tttggcttcc gccatgttgg ccggagacag cgtaaacagg
    10861 cagctaaagg caaagaacat caccactgca accatcagca tgctggcacg agaaatgatt
    10921 tgggaacatt tacgttcggt gaagtcgcga ccgaagtctt tctcatactc ttcacgttta
    10981 gaaaccacga aggaagagac gattggcgag aagttaaagg agaaaaccat gatggaaatc
    11041 cccagccaga cagtgatcag gataccgtca tgaccggtta acgacagcga accgaggtca
    11101 acctggtcga taactgcaga gttccagtaa gggatcagcg acagagaaat cagcaccagg
    11161 ctggcgataa acggccatac caggtagctc atcactttaa ccatcagatc cttaccaaac
    11221 cagatgacga aagccatcag cagcaacagg aacagcgcca caaagccgcg attcagcggt
    11281 gcaaagccga gctggttttc ccagaacgtc ataaaggtat tggtaatagt aacgccataa
    11341 atccacagca gtgggcaaat cgcgaagaag tacaggaacg tgataaccac gccgccagtt
    11401 ttaccaaaat gctcttccac cgtttccgta atgttgccgg aagggttaga gccagaaaga
    11461 cacagacgcg ccagcgcccg gtggcaataa aatgcgatgg ggtatgccaa taccaacatc
    11521 agaagaatcg ggatcagtcc gccaaaacct gcgcggatag ggaagaacag caccccggcg
    11581 ccgattgccg taccaaacaa gcctaacgtc catgtggtat ctgatttacg ccaggacgat
    11641 tgttttgtct ggctggatac aatgctatct gaagtactca tatcctatcc tcaacgaatt
    11701 aattaagcgt caacgaaacc ggtgatttga gagacgcgag aaagatcgat attgccgccg
    11761 gaaataatac tgacggtttt tctgttttga atatattggt ctaatttacc gcttaataat
    11821 gcagcacatg ccagagcgcc tgcgccttcg gtgacgactt tattgcgctg aattaaggca
    11881 atcatactgt ttctgatttc atcttcgctg accagcacga tgtcatcgac taattcacga
    11941 acgatttcgt aagttaaatt acccgggcgg gagacatcac aaccatccgc cagggtgccg
    12001 gtagttcggt gcgtggttat ttctccggag tggaaagaag ccgccatgcc gtgaacgttt
    12061 tcagactgta cgccaataac acgaatggtc gggttaatag atttaattgc caccgcaata
    12121 ccagcaatta aaccgccacc gccaattggc acaatcacat tatcgacatc atagagatct
    12181 tccataattt ccagaccaat cgttccctgg ccagcaatca ctttcggatc atcgtaaggt
    12241 gggataaaaa tacggccttc catttcgaca atttcgctca ctttagcaat agtgtcgttg
    12301 aagttatcac catgcaaaac gacttctgcg gagtagtcgc acgttgccgc gactttggat
    12361 ttcggcgcac cttttggcat caccacttta ccgtcaatac ccagcatcgc acaggagagc
    12421 gaaacccctt gcgcatggtt gcccgcagag caggccacta cacctttacg tttttccgca
    12481 tcagtcagtg aacttaattt attaaatgcg ccacgaattt taaatgaacc ggtacgctgc
    12541 atgttttcga atttgaggaa tatttcacct ttgcaacgtt cactaaaata gttggagcga
    12601 ggcatacctg ttttataaat tcgcccagcc agtcgttgtt ttgcttcaat gatgtcatca
    12661 atagcaaccg ggagatcgta tgtaatatgc attataaaac ctcttagcct gaattaaata
    12721 tgtaggtaac cgtaaccgac acctgcaaaa cagacaggtg gattatttat gtttaaatta
    12781 aaacaaataa ctaatcaact tcaattaatt gccttcgtct acacccatta taagatgaat
    12841 actctttggc taattccacc aaaaccgatg ctgctttttt aatacgataa tttttcgacc
    12901 ataccgcggc atattgtgcc acaggtaatg tttcttcaac cggaatagta ataaattgat
    12961 tagaaccaaa aggtgatgtc atatcacaag gaattacagt taagaaatca gcattgagaa
    13021 caagattata aattgtcacg actgagtcgg ttttaacgat gttttcaata ctgatgccat
    13081 ttctttgtaa cgtagtaagt agttcgctgt agtaccccat attggtttgt ggcaacaccc
    13141 actgttcgtt cttcaacgac tccagcgtgg tggtgccggt gcatgttcgg gacttactgg
    13201 ctaccagcac aaactcggac tcgaacagcg gctcaacatg taaatcctga agcttcattt
    13261 ctgcgcttaa cgtaccaatc gcaaaatcca gtcgaccgtc gcgtattgcc ggtaagaacg
    13321 aagacagttg cgcttcatac atagaaacct gcgctttcgg gaacacctct ttgaacttgt
    13381 tgatcatccc tgacataaaa gtaaaaccaa tcaatgaagg aaaaccaaat gagacttcca
    13441 ccaccgcctc agaagacata ccgcttatct cattaaccat atttttcatt tcacgggtaa
    13501 tggattcgga acgggagagt aacaattgac cagcaggtgt taatgttaca ccggtatttt
    13561 tccgcaccac taactccaca ccaaaataat cttcaatatc gttaatgatt ttactgacgg
    13621 ccggttgagt taaccctaac tcttttgcag ccgagccgat agaaccactt ctaataactt
    13681 cctgaaagac taccaggtgc tgcgttttcg gaagaagaat agtgctcata acgacctacg
    13741 ttaattacct cattgacggc atgaagtgta tcaaaatgaa atgaacagga tatgtgcgac
    13801 cactcacaaa ttaactttca atactttcca gg
//
LOCUS       DAPHCV010000118        13419 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.118, whole genome shotgun sequence.
ACCESSION   DAPHCV010000118 DAPHCV010000000
VERSION     DAPHCV010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.118"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(266..1549)
                     /gene="gltA"
                     /locus_tag="RH033_003795"
     CDS             complement(266..1549)
                     /gene="gltA"
                     /locus_tag="RH033_003795"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HDV2992917.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1755..1862)
                     /locus_tag="RH033_003796"
     CDS             complement(1755..1862)
                     /locus_tag="RH033_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992918.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2243..2647
                     /gene="sdhC"
                     /locus_tag="RH033_003797"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2243..2647
                     /gene="sdhC"
                     /locus_tag="RH033_003797"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HDV2992919.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2641..2988
                     /gene="sdhD"
                     /locus_tag="RH033_003798"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2641..2988
                     /gene="sdhD"
                     /locus_tag="RH033_003798"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HDV2992920.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2988..4754
                     /gene="sdhA"
                     /locus_tag="RH033_003799"
     CDS             2988..4754
                     /gene="sdhA"
                     /locus_tag="RH033_003799"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HDV2992921.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4770..5486
                     /gene="sdhB"
                     /locus_tag="RH033_003800"
     CDS             4770..5486
                     /gene="sdhB"
                     /locus_tag="RH033_003800"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HDV2992922.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5847..8648
                     /gene="sucA"
                     /locus_tag="RH033_003801"
     CDS             5847..8648
                     /gene="sucA"
                     /locus_tag="RH033_003801"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HDV2992923.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8663..9880
                     /gene="odhB"
                     /locus_tag="RH033_003802"
     CDS             8663..9880
                     /gene="odhB"
                     /locus_tag="RH033_003802"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HDV2992924.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9974..11140
                     /gene="sucC"
                     /locus_tag="RH033_003803"
     CDS             9974..11140
                     /gene="sucC"
                     /locus_tag="RH033_003803"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HDV2992925.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            11140..12009
                     /gene="sucD"
                     /locus_tag="RH033_003804"
     CDS             11140..12009
                     /gene="sucD"
                     /locus_tag="RH033_003804"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HDV2992926.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            12249..13364
                     /locus_tag="RH033_003805"
     CDS             12249..13364
                     /locus_tag="RH033_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDV2992927.1"
                     /translation="MGVNSDVYAADVNIDILSATVKDKRIEGVSVTLQRNGAQSVSGT
                     TNASGSVNLGSTFADDQDALLIVKKEGYSNLVVKCSCAGMTYAISPAMTSLDGMRVVL
                     SWGEKPFDLDSHLIFPGGHIYFDSKEGTDANLDVDDTDSYGSETVTISKKHFGESYIY
                     AVQDYSNKGLPNSNYLSASKAKVFVYVGSSLVRSYSVPAGKRGNIWTVFKLNPNGEFE
                     DINSVTSANFNDTTLGVRDLATVIMPATGSSTPASPAMQNSGDTQLARKYNREGEAVY
                     KTGQLEQAIQLFQQATELDGNYGQAFSNLGLAYQKNGNIAEAIWANRKAISLASGANA
                     ATMRANSYYNIAKIYETAGQDADALQYYQLALHDAIL"
BASE COUNT         3302 a         3337 c         3624 g         3156 t
ORIGIN      
        1 agtgcataaa tttattgttt aatgagatga aagatggggt gggatggata tgcataattt
       61 tcattgggtt aacaatgcta ccggcaggaa agtaaaaaaa taaacttatc attctgctca
      121 gtatgtgact tgtcacattt tttcacctta ttaatgggta tttgcatctg gtttaatttt
      181 tacaatttat tatgttcagc catataaaaa agaacccgct ctattaaagg cgggtccgga
      241 aagtaaacgg cttagcaatc aatcattaac gcttgatatc gcttttaaag tcgcgttttt
      301 catatcctgt atacagttga cgcggacggg caatcttcat accgtcactg tgcatttcgc
      361 tccagtgggc gatccagcca acggtacgtg ccattgcgaa aatgacggtg aacatggaag
      421 acggaatacc catcgctttc aggatgatac cagagtagaa atcgacgttc gggtacagtt
      481 ttttctcgat aaagtacggg tcgttcagcg cgatgttttc cagctccata gccacttcca
      541 gcaggtcatc tttggtaccc agctctttca gaacttcatg gcaggtttca cgcattacgg
      601 tggcgcgcgg gtcgtaattt ttgtacacgc ggtgaccgaa gcccatcagg cggaaagaat
      661 catttttgtc tttcgcacga cgaacaaatt ccggaatgtg tttaacggag ctgatttctt
      721 ccagcatttt cagcgccgct tcgttagcac caccgtgcgc aggtccccac agtgaagcaa
      781 tacctgcagc gatacaggca aacgggttcg cacccgaaga gccagcggta cgcacggtgg
      841 aggtggaggc gttctgttca tggtcagcgt gcaggatcag aatacggtcc atagcacgtt
      901 ccagaatcgg attaacttca tacggttcgc acggcgtgga gaacatcata ttcaggaagt
      961 taccagcgta ggagagatca ttgcgcgggt aaacaaatgg ctgaccaatg gaatacttgt
     1021 aacacatcgc ggccatggtc ggcatttttg acagcaggcg gaacgcggca atttcacgat
     1081 gacgaggatt gttaacatcc agcgagtcgt ggtagaacgc cgccagcgcg ccggtaatac
     1141 cacacatgac tgccattgga tgcgagtcgc gacggaaagc atggaacaga cgggtaatct
     1201 gctcgtggat catggtatga cgggtcaccg tagttttaaa ttcgtcatac tgttcctgag
     1261 tcggtttttc accattcagc aggatgtaac aaacttccag gtagttagaa tcggtcgcca
     1321 gctgatcgat cgggaaaccg cggtgcagca aaataccttc atcaccatca ataaaagtaa
     1381 ttttagattc gcaggatgcg gttgaagtga agcctgggtc aaaggtgaat acaccttttg
     1441 aaccgagagt acggatatca ataacatctt gacccagcgt gcctttcagc acatccagtt
     1501 caacagctgt atccccgttg agggtgagtt ttgcttttgt atcagccatt taaggtctcc
     1561 ttagcgcctt attgcgtaag actgccggaa cttaaatttg ccttcgcaca tcaacctggc
     1621 tttacccgtt tgttatttgg ctcgccgctc tgtgaaagag gggaaaacct gggtacagag
     1681 ctctgggcgc ttgcaggtaa aggatccatt gatgacgaat aaatggcgaa tcaagtactt
     1741 agcaatccga attattaaac ttgtctacca ctaataactg tcccgaatga attggtcaat
     1801 acttccacac tgttacataa gttaatctta ggtgaaatac cgacttcata acttttacgc
     1861 attatatgct tttcctggta atgtttgtaa caactttgtt gaatgattgt caaattagat
     1921 gattaaaaat taaataaatg ttgttatcgt gacgcagatc actgttcagg ataaaacccg
     1981 acaaactata tgtaggttaa ttgtaatgat tttgtgaacg gcctatactg ccgccaggtc
     2041 tccggaacac cctgcaatcc cgagccaccc agcgttgtaa cgtgtcgttt tcgcatctgg
     2101 aagcagtgtt ttgcatgacg cgcagttata gaaaggacgc tgtctgaccc gcaagcagac
     2161 cggaggaagg aaatcccgac gtctccaggt aacagaaagt taacctctgt gcccgtagtc
     2221 cccagggaat aataagaaca gcatgtgggc gttattcatg ataagaaatg tgaaaaaaca
     2281 aagacctgtt aatctggacc tacagaccat ccggttcccc atcacggcga tagcgtccat
     2341 tctccatcgc gtttccggtg tgatcacctt tgttgcagtg ggcatcctgc tgtggcttct
     2401 gggtaccagc ctctcttccc ctgaaggttt cgagcaagct tccgcgatta tgggcagctt
     2461 cttcgtcaaa tttatcatgt ggggcatcct taccgctctg gcgtatcacg tcgtcgtagg
     2521 tattcgccac atgatgatgg attttggcta tctggaagaa acattcgaag cgggtaaacg
     2581 ctccgccaaa atctcctttg ttattactgt cgtgctttca cttctcgcag gagtcctcgt
     2641 atggtaagca acgcctccgc attaggacgc aatggcgtac atgatttcat cctcgttcgc
     2701 gctaccgcta tcgtcctgac gctctacatc atttatatgg tcggtttttt cgccaccagt
     2761 ggcgaactga catatgaagt ctggatcggt ttcttcgcct ctgcgttcac caaagtgttc
     2821 accctgctgg cgctgttttc tatcttgatc catgcctgga tcggcatgtg gcaggtgttg
     2881 accgactacg ttaaaccgct ggccttgcgc ctgatgctgc aactggtgat tgtcgttgct
     2941 ctggtggttt acgtgattta tggattcgtt gtggtgtggg gtgtgtgatg aaattgccag
     3001 tcagagaatt tgatgcagtt gtgattggtg ccggtggcgc aggtatgcgc gcggcgctgc
     3061 aaatttccca gagcggtcag acctgtgcgc tgctctctaa agtcttcccg acccgttccc
     3121 ataccgtttc tgcgcaaggc ggcattaccg ttgcgctggg taatacccat gaagataact
     3181 gggaatggca tatgtacgac accgtaaaag gttcggacta tatcggtgac caggacgcga
     3241 ttgaatatat gtgtaaaacc gggccggaag cgattctgga actggaacat atgggcctgc
     3301 cgttctcgcg tcttgatgat ggtcgtatct atcaacgtcc gtttggcggt cagtcgaaaa
     3361 acttcggcgg cgagcaggcg gcacgtactg cggcggcagc tgaccgtacc ggtcacgcac
     3421 tgttgcacac cctttatcag cagaacctga aaaaccacac caccattttc tccgagtggt
     3481 atgcgctgga tctggtgaaa aaccaggatg gcgcggtggt cggttgtacc gcactgtgca
     3541 tcgaaactgg tgaagtggtt tacttcaaag ctcgcgctac cgtgctggcg actggcgggg
     3601 cagggcgtat ttatcagtcc actaccaacg cccacattaa cactggcgac ggcgtcggca
     3661 tggctatccg tgcaggcgta ccagtgcagg atatggaaat gtggcagttc cacccgaccg
     3721 gtattgccgg tgcgggcgta ctggtcaccg aagggtgccg tggtgaaggc ggttatctgc
     3781 tgaacaaaca tggcgaacgc tttatggagc gttatgcgcc gaacgccaaa gacctggcgg
     3841 gccgtgacgt ggtggcgcgt tccatcatga ttgaaatccg tgaaggtcgc ggctgtgatg
     3901 gtccgtgggg gccacacgcg aaactgaaac ttgaccactt gggtaaagaa gttctcgaat
     3961 cccgtctgcc gggtattctg gaactctccc gtaccttcgc tcacgttgat ccggtgaaag
     4021 agccgattcc ggttatccca acctgtcact acatgatggg cggtattccg accaaagtga
     4081 ccggtcaggc gctgacggtg aacgagaaag gcgaagatgt ggttgttccg gggctgtttg
     4141 ccgttggtga aatcgcttgt gtatcggtac acggcgctaa ccgtctgggc ggcaactcgc
     4201 tgctggacct ggtggtcttt ggtcgcgcag cgggtttgca tctgcaagag tctatcgccg
     4261 agcagggcgc attgcgcgat gccagcgagt ctgatgttga agcatctctg gatcgtctga
     4321 accgctggaa caataatcgt aacggtgaag atccggtggc gattcgtaaa gcgctgcaag
     4381 aatgtatgca gcataacttc tcggtcttcc gtgaaggtga tgcgatggcg aaagggcttg
     4441 agcagttgaa agttatccgc gagcgtctga aaaatgcccg tctggatgac acttccagcg
     4501 agttcaacac ccagcgcgtt gagtgcctgg aactggataa cctgatggaa acggcgtatg
     4561 caacggctgt ttctgccaac ttccgtaccg aaagccgtgg cgcacatagc cgcttcgact
     4621 tcccggatcg tgatgatgaa aactggctgt gccactccct gtatctgcct gagtcggaat
     4681 ccatgacgcg ccgaagcgtc aacatggaac cgaaactgcg cccggcattc ccgccgaaga
     4741 ttcgtactta ctaatgcgga gacaggaaaa tgagactcga gttttcaatt tatcgctata
     4801 acccggatgt tgatgatgct ccgcgtatgc aggattacac cctggaagcg gaagaaggtc
     4861 gtgacatgat gctgctggat gcgcttatcc agctgaaaga gaaagatccc agcctgtcgt
     4921 tccgtcgctc ctgccgtgaa ggtgtgtgcg gttccgacgg tctgaacatg aacggcaaga
     4981 atggtctggc ctgtattacc ccgatttcgg cactcaacca gccgggcaag aagattgtga
     5041 ttcgcccgct gccaggttta ccggtgatcc gcgatttggt ggtagacatg ggacaattct
     5101 atgcgcaata tgagaaaatt aagccttacc tgttgaataa tggacaaaat ccgccagctc
     5161 gcgagcattt acagatgcca gagcagcgcg aaaaactcga cgggctgtat gaatgtattc
     5221 tctgcgcatg ttgttcaacc tcttgtccgt ctttctggtg gaatcccgat aagtttatcg
     5281 gcccggcagg cttgttagcg gcatatcgtt tcctgatcga tagccgtgat accgagactg
     5341 acagccgcct cgacggtttg agcgatgcat tcagcgtatt ccgctgtcac agcatcatga
     5401 actgcgtcag tgtatgtccg aaggggctga acccgacgcg cgccatcggc catatcaagt
     5461 cgatgttgtt gcaacgtaat gcgtaaaccc aggcctgata agacgcacaa gcgtcgcatc
     5521 aggcaaccag tgccggatgc ggcgtgaacg ccttatccgg cctacaaaag aaatgcagaa
     5581 accgtaggcc tgataagacg cgcaagcgtc gcatcaggca aacagcgccg gatgcggcgt
     5641 gaatgcctta tccggcctac aaaagaaatg caggaaatct ttaaaaactg cccctgacac
     5701 taagacagtt tttaaaggtt ccttagcgga cgcagaaaac acgacaagtc cgcaacaagt
     5761 gaaccccggc acgcacatca gatatgaaat gtgcgtggta gtatccacgg cgaagtaagc
     5821 ataaaaaaga tgcttaaggg atcacgatgc agaacagcgc tttgaaagcc tggttggact
     5881 cttcttacct ctctggcgca aaccagagct ggatagaaca gctctatgaa gacttcttaa
     5941 ccgatcctga ctcggttgac gctaactggc gttcgacgtt ccagcagtta cctggtacgg
     6001 gagtcaaacc ggatcaattc cactctcaaa cgcgtgaata tttccgccgc ctggcgaaag
     6061 acgcttcacg ttactcttca acgatctccg accctgacac caatgtgaag caggttaaag
     6121 tcctgcagct cattaacgca taccgcttcc gtggtcacca gcatgcgaat ctcgatccgc
     6181 tgggactgtg gcagcaagat aaagtggccg atctggatcc atctttccac gatctgaccg
     6241 aagcagactt ccaggagacc ttcaacgtcg gttcatttgc cagcggcaaa gaaaccatga
     6301 aactcggcga gctgctggaa gccctcaagc aaacctactg cggcccgatt ggtgccgagt
     6361 atatgcacat caccagcacc gaagaaaaac gctggatcca acagcgtatc gagtctggtc
     6421 gcgcgacttt caatagcgaa gagaaaaaac gcttcttaag cgaactgacc gccgctgaag
     6481 gccttgaacg ttacctcggc gcaaaattcc ctggcgcaaa acgcttctcg ctggaaggcg
     6541 gtgacgcgtt aatcccgatg cttaaagaga tgatccgcca cgctggcaac agcggcaccc
     6601 gcgaagtggt tctcgggatg gcgcaccgtg gtcgtctgaa cgtgctggtg aacgtgctgg
     6661 gtaaaaaacc gcaagacttg ttcgacgagt tcgccggtaa acataaagaa cacctcggca
     6721 cgggtgacgt gaaataccac atgggcttct cgtctgactt ccagaccgat ggcggcctgg
     6781 tgcacctggc gctggcgttt aacccgtctc accttgagat tgtcagcccg gtagttatcg
     6841 gttctgttcg tgcccgtctg gacagacttg atgagccgag cagcaacaaa gtgctgccaa
     6901 tcaccatcca cggtgacgcc gcagtgaccg gacagggcgt ggttcaggaa accctgaaca
     6961 tgtcgaaagc gcgtggctat gaagttggcg gtacggtacg tatcgttatc aacaaccagg
     7021 ttggcttcac cacctctaac ccgctggatg cccgttcgac gccgtactgt actgatatcg
     7081 gtaagatggt tcaggcaccg attttccacg taaacgcgga tgatccggaa gccgttgcct
     7141 ttgtgacccg tctggcgctc gatttccgta acacctttaa acgtgatgtc ttcatcgacc
     7201 tggtgtgcta ccgccgtcat ggccacaacg aagccgacga gccgagcgca acccagccgc
     7261 tgatgtatca gaaaatcaaa aaacatccga cgccgcgcaa aatctacgct gacaagctgg
     7321 agcaggaaaa agtggcgacg ctggaagatg ccaccgagat ggttaacctg taccgcgatg
     7381 cgctggatgc tggcgattgc gtggtagcag agtggcgtcc gatgaacatg cactctttca
     7441 cctggtcgcc atacctcaac cacgaatggg acgaagagta cccgaacaaa gttgagatga
     7501 agcgcctgca ggaactggct aaacgcatca gtacggtgcc ggaagcagtt gaaatgcagt
     7561 ctcgcgttgc caagatttat ggcgatcgcc aggcaatggc agccggtgag aaactgttcg
     7621 actggggcgg tgcggaaaac ctcgcttacg ccacgctggt tgatgaaggc attccggttc
     7681 gcctgtcggg tgaagactcc ggtcgcggta ccttcttcca ccgccacgcg gtgatccaca
     7741 accagtctaa cggttccact tacacgccgc tgcaacacat ccataacggt cagggcgcgt
     7801 tccgtgtctg ggactccgta ctgtcagaag aagccgtgct ggcgtttgaa tacggttatg
     7861 ccaccgcaga accacgcact ctgaccatct gggaagcgca gttcggtgac ttcgccaacg
     7921 gtgcgcaggt ggttatcgac cagttcatct cctctggcga acagaaatgg ggccggatgt
     7981 gtggtctggt gatgttgctg ccgcacggtt acgaagggca ggggccggag cactcctccg
     8041 cgcgtctgga acgttatctg caactttgtg ctgagcaaaa catgcaggtt tgcgtacctt
     8101 ctaccccggc acaggtttac cacatgctgc gtcgtcaggc gctgcgcggg atgcgtcgtc
     8161 cgctggtcgt gatgtcgccg aaatccctgc tgcgtcatcc gctggcggtt tccagcctcg
     8221 aagaactggc gaacggcacc ttcctgccag ccatcggtga aatcgacgag cttgatccga
     8281 agggcgttaa gcgtgtagtg atgtgttctg gtaaggttta ttacgacctg ctggaacaac
     8341 gtcgtaagaa caatcaacac gatgtcgcca ttgtgcgtat cgagcaactc tacccgttcc
     8401 cgcataaagc gatgcaggaa gtgttgcagc agtttgctca cgtcaaggat tttgtctggt
     8461 gccaggaaga gccgctcaac cagggcgcat ggtactgcag ccagcatcat ttccgtgaag
     8521 tgattccgtt tggggcttct ctgcgttatg caggccgccc ggcctccgcc tctccggcgg
     8581 tagggtatat gtccgttcac cagaaacagc aacaagatct ggttaatgac gcgctgaacg
     8641 tcgaataaat aaaggataca caatgagtag cgtagatatt ctggtccctg acctgcctga
     8701 atccgtagcc gatgccaccg tcgcaacctg gcataaaaaa cccggcgacg cagtcgtacg
     8761 tgatgaagtg ctggtagaaa tcgaaactga caaagtggta ctggaagtac cggcatcagc
     8821 agacggcatt ctggatgcgg ttctggaaga tgaaggtaca acggtaacgt ctcgtcagat
     8881 ccttggtcgc ctgcgtgaag gcaacagcgc gggtaaagaa accagcgcga aatctgaaga
     8941 gaaagcgtcc actccggcgc aacgccagca ggcgtctctg gaagagcaaa acaacgatgc
     9001 gttaagcccg gcgatccgtc gcctgctggc tgaacataac ctcgacgcca gcgccattaa
     9061 aggcaccggt gtgggtggtc gtctgacccg tgaagatgtg gaaaaacatc tggcgaaagc
     9121 cccggcgaaa gagtctgctc cggcagcggc tgctccggcg gcgcaaccgg ccctggctgc
     9181 acgtagcgaa aaacgtgtcc cgatgacccg cctgcgtaag cgtgtggcag agcgtctgct
     9241 ggaagcgaaa aactccaccg ccatgctgac cacgttcaac gaagtcaaca tgaagccgat
     9301 tatggatctg cgtaagcagt acggtgaagc gtttgaaaaa cgccacggca ttcgtctggg
     9361 ctttatgtcc ttctacgtga aagcggtggt tgaagccctg aaacgttacc cggaagtgaa
     9421 tgcgtctatc gacggcgatg atgtggttta ccacaactat ttcgacgtca gtatggcggt
     9481 ttctacgccg cgcggcctgg tgacgccggt tctgcgtgat gtcgataccc tcggcatggc
     9541 agacatcgag aagaaaatca aagagctggc agttaaaggc cgtgacggca agctgacggt
     9601 tgaagatctg accggtggta acttcaccat caccaacggt ggtgtgttcg gttccctgat
     9661 gtcaacgcct atcatcaacc cgccgcagag cgcaattctg ggtatgcacg ctatcaaaga
     9721 tcgtccgatg gcggtgaatg gtcaggttga gatcctgccg atgatgtacc tggcgctgtc
     9781 ctacgatcac cgtctgatcg atggtcgcga atccgtgggc ttcctggtaa cgatcaaaga
     9841 gttgctggaa gatccgactc gtctgctgct ggacgtgtag tagtttaagc ttcacctgca
     9901 ctgtagaccg acgtgaatag ggtgtcggtc tacggtttaa aagataacga ttactgaagg
     9961 atggacagaa cacatgaact tacatgaata tcaggcaaaa caactttttg cccgctatgg
    10021 cttaccagca ccggtgggtt atgcctgtac tactccgcgc gaagcagaag aagccgcttc
    10081 aaaaatcggt gccggtccgt gggtagtgaa atgtcaggtt cacgctggtg gccgcggtaa
    10141 agcgggcggt gtgaaagtgg taaacagcaa agaagacatc cgtgcttttg cagaaaactg
    10201 gctgggcaag cgtctggtaa cgtatcaaac agatgccaat ggccaaccgg ttaaccagat
    10261 tctggttgaa gcagcgaccg atatcgctaa agagctgtat ctcggtgccg ttgttgaccg
    10321 tagttcccgt cgtgtggtct ttatggcctc caccgaaggc ggcgtggaaa ttgaaaaagt
    10381 ggcggaagaa actccgcacc tgatccacaa agttgcgctt gatccgctga ctggcccgat
    10441 gccgtatcag ggacgcgagc tggcgttcaa actgggtctg gaaggtaaac tggttcagca
    10501 gttcaccaaa atcttcatgg gcctggcgac cattttcctg gagcgcgacc tggcgctgat
    10561 cgaaatcaac ccgctggtca ttaccaaaca gggcgatctg atttgcctcg acggcaaact
    10621 gggcgctgat ggcaacgcac tgttccgcca gcctgatctg cgcgaaatgc gtgaccagtc
    10681 gcaggaagat ccgcgtgaag cacaggctgc acagtgggaa ctgaactacg ttgcgctgga
    10741 cggtaacatc ggttgtatgg ttaacggcgc aggcctggcg atgggcacca tggacatcgt
    10801 taaactgcac ggcggcgaac cagctaactt cctggacgtt ggcggcggcg caaccaaaga
    10861 acgtgtaacc gaagcgttca aaatcatcct ctctgacgac aaagtgaaag ccgttctggt
    10921 taacatcttc ggcggtatcg ttcgttgcga cctgatcgct gacggtatca tcggcgcggt
    10981 agcggaagtg ggtgttaacg taccggtcgt ggtacgtctg gaaggtaaca acgccgaact
    11041 cggcgcgaag aaactggctg acagcggcct gaatattatt gcagcaaaag gtctgacgga
    11101 tgcagctcag caggttgttg ccgcagtgga ggggaaataa tgtccatttt aattgataaa
    11161 aacaccaagg ttatctgcca gggctttacc ggtagccagg ggactttcca ctcagaacag
    11221 gccattgcat acggcactaa aatggttggc ggcgtaaccc caggtaaagg cggtactacc
    11281 cacctcggcc tgccagtgtt caacaccgtg cgtgaagctg ttgctgctac tggcgctacc
    11341 gcctctgtta tctacgtacc agcaccgttc tgcaaagact ccattctgga agctatcgat
    11401 gcaggcatca aactgattat caccatcact gaaggcatcc cgacgctgga tatgctgacg
    11461 gtgaaagtga aactggatga agcaggcgtt cgtatgatcg gcccgaactg cccaggcgtt
    11521 atcaccccgg gggaatgcaa aatcggtatc cagccgggtc acattcacaa accgggtaaa
    11581 gtgggtatcg tttcccgttc cggtacgctg acctatgaag cggttaaaca gaccacggat
    11641 tacggtttcg gtcagtcgac ctgcgttggt atcggcggcg acccgatccc tggctctaac
    11701 tttatcgaca tcctcgaaat gttcgaaaaa gatccgcaga ccgaagcgat cgtgatgatc
    11761 ggtgagatcg gcggtagcgc tgaagaagaa gcagcggcgt acatcaagga gcacgttacc
    11821 aagccagttg tgggttacat cgctggtgtg actgcgccga aaggcaaacg tatgggtcac
    11881 gcaggtgcga tcattgccgg tggtaaaggg actgcggatg agaaattcgc tgctctggaa
    11941 gccgcaggcg tgaaaaccgt tcgcagcctg gcggatatcg gtgaagcact gaaaactgtt
    12001 ctgaaataaa tatctgtaat aagaaatagc cctcgccgct tccctctaca ggaggggcga
    12061 agggctgtcg gtttcgacat ggttggccat cttatgatgg ccttttttat tcgggcacta
    12121 atctgaaaag tttgcccact gcttttctcc catcttctgc gcaataatgc ccggcaaaat
    12181 atcccctctc cgttctgtaa aagcatttat acaagaagaa aattgttctg ccggttgctc
    12241 tggccctgat gggtgtaaat tcggacgttt atgcggccga tgtaaatatc gatattttaa
    12301 gtgcgacagt gaaagataaa cgcattgagg gagtatcggt gacgctgcaa cgcaatggcg
    12361 cacaatctgt ttctggaacc acaaatgcat cgggcagcgt taatctgggt tctacatttg
    12421 ctgatgatca agatgcttta ttgatcgtca aaaaagaggg ctattctaat ctggtggtca
    12481 aatgttcgtg tgctggcatg acttatgcga ttagtccggc aatgactagt ctggatggca
    12541 tgcgtgtagt tttgagctgg ggcgaaaagc cttttgatct tgattcacat ttgatcttcc
    12601 ctggcggaca tatctatttt gatagtaaag aggggactga tgctaatctg gatgttgatg
    12661 atacggatag ctatggatca gaaactgtca ctatcagcaa aaagcatttt ggcgaaagtt
    12721 acatctatgc cgtgcaggac tatagcaaca aagggttgcc caacagtaat tatctctctg
    12781 ccagtaaggc aaaagtgttt gtttatgtag ggagctccct ggtgcgtagt tatagtgtcc
    12841 ctgctggaaa acgagggaac atctggaccg tgtttaaact caatccaaac ggcgagtttg
    12901 aagatatcaa ctctgttacc agcgccaatt ttaacgacac cacattaggc gtcagggatt
    12961 tagccactgt gataatgcct gcgacgggtt cttcaactcc ggcttcacct gcgatgcaaa
    13021 acagtggaga tactcaactg gcaagaaaat ataatcgcga aggtgaagct gtttataaaa
    13081 ccggtcaact tgagcaagcc attcaactct tccaacaggc aacagaatta gacggcaatt
    13141 acggacaggc atttagtaac ctggggctgg cgtatcagaa aaatggcaac atcgcggaag
    13201 caatttgggc aaatcgtaaa gcgatctcgt tagccagtgg agcaaatgcg gcgacaatgc
    13261 gcgctaactc ctattataac attgccaaaa tttatgaaac tgctggacag gacgctgatg
    13321 cattgcagta ttatcaactc gctcttcacg atgccatttt ataacaatga aagtggaatc
    13381 atatttttaa attttatatt cttgcttgtt tttctggtg
//
LOCUS       DAPHCV010000119        13414 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.119, whole genome shotgun sequence.
ACCESSION   DAPHCV010000119 DAPHCV010000000
VERSION     DAPHCV010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.119"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(58..831)
                     /gene="uxuR"
                     /locus_tag="RH033_003806"
     CDS             complement(58..831)
                     /gene="uxuR"
                     /locus_tag="RH033_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HDV2992928.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPEANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1045..2505)
                     /locus_tag="RH033_003807"
     CDS             complement(1045..2505)
                     /locus_tag="RH033_003807"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HDV2992929.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGKAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(2586..3770)
                     /gene="uxuA"
                     /locus_tag="RH033_003808"
     CDS             complement(2586..3770)
                     /gene="uxuA"
                     /locus_tag="RH033_003808"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDV2992930.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            4110..5453
                     /gene="gntP"
                     /locus_tag="RH033_003809"
     CDS             4110..5453
                     /gene="gntP"
                     /locus_tag="RH033_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HDV2992931.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQI
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(5627..6529)
                     /gene="fimH"
                     /locus_tag="RH033_003810"
     CDS             complement(5627..6529)
                     /gene="fimH"
                     /locus_tag="RH033_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HDV2992932.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(6549..7052)
                     /gene="fimG"
                     /locus_tag="RH033_003811"
     CDS             complement(6549..7052)
                     /gene="fimG"
                     /locus_tag="RH033_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HDV2992933.1"
                     /translation="MKWRKRGYLLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNSGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(7065..7595)
                     /gene="fimF"
                     /locus_tag="RH033_003812"
     CDS             complement(7065..7595)
                     /gene="fimF"
                     /locus_tag="RH033_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HDV2992934.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(7605..10241)
                     /gene="fimD"
                     /locus_tag="RH033_003813"
     CDS             complement(7605..10241)
                     /gene="fimD"
                     /locus_tag="RH033_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HDV2992935.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAVQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSMIHDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGSNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTNNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(10308..11033)
                     /gene="fimC"
                     /locus_tag="RH033_003814"
     CDS             complement(10308..11033)
                     /gene="fimC"
                     /locus_tag="RH033_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HDV2992936.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(11070..11609)
                     /locus_tag="RH033_003815"
     CDS             complement(11070..11609)
                     /locus_tag="RH033_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV2992937.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(11674..12222)
                     /gene="fimA"
                     /locus_tag="RH033_003816"
     CDS             complement(11674..12222)
                     /gene="fimA"
                     /locus_tag="RH033_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDV2992938.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATHTDVLALQSSAAGSATNVGVQILDRTGAALALDGATFSSETTLNNGTNTIPFQARY
                     FATGAATPGAANADATFKVQYQ"
     gene            complement(12704..13300)
                     /gene="fimE"
                     /locus_tag="RH033_003817"
     CDS             complement(12704..13300)
                     /gene="fimE"
                     /locus_tag="RH033_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HDV2992939.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEI"
BASE COUNT         3222 a         3484 c         3251 g         3457 t
ORIGIN      
        1 ctattcaggg aacatacata gtgcaaagcg gttcaacttt gcaccagaaa tgagggatca
       61 ggcgtcgact ttatccagcg gccatgaatc aaacagatag ccatcgaaat agatgtcatc
      121 aacattagag aactccagca agcgctgctt cacattctcc agatgctgcc acattgccag
      181 cttcgccgct cgggcatctt ttttgattaa cgcggcgagg atctgtttgt gatcgcccaa
      241 ccactctttg cgatacaggc tgtcatcgag atggctgtgc aattggatcc acatcggatt
      301 gttttcccgc cactgccagg actgacggaa tagctccacc agcatgctgt tatgcgtcgc
      361 ttcggcaatg gcgagatgga actgcatgtc accgctttcg ctgctgcccg gcgcgctgga
      421 agccagttca cgctcttcca gttgcaatgc ctgacgcatt ttgacgatat cttcgcgggt
      481 agcctgcagg gcggcaaact cggcgatgtt actttccagt aactgacgcg cctgtaacag
      541 ctcaaaagga cccgcatcat tgcaaacgtt ggcctccgga ctgtctgtgt tgtggctgcc
      601 tgagctatca agaacataaa taccggcacc ccggcgcact tccaccagcc ctttgatctc
      661 cagcatgatc agcgcttcac gtaccaccgt ccgcgtgaca tcaagcattt ctgcaatttc
      721 acgctccggc ggcagccgtt cgccaggatt gtacggcgtc tttatgatca gatcgcggat
      781 catcgcccca acttcctggt aaggtctttg cgcagaggtg gcagatttca tggtgttgtg
      841 tccgttaggt gcggttaatc aaattttgcc ggatgcggcg tgaatgcctt atccggccta
      901 caacgggcaa ttttacggca cccgttaatc tcaaactata ccattagcac tggaagttgg
      961 cacgtacagg tgggcaaaaa aacgaaaact ggtcaaccaa atatgcgttt gattgaccag
     1021 taagtctgta gggtaatcag ttagttacag cgcagccaca cactcgcgcg caccgcgttc
     1081 gcacagctgc tgatatgccg ccgtcaccgc gccaacaaag tcggcattct gcggcagatc
     1141 atcggcaaag ataccgctca ggcccagcag cgctttcacg cggtctgcgc cctgatactg
     1201 cgcgttgatc ttctggaact ccgccagcat cgggtcgacc acgtcaatag ctttaccctg
     1261 ctcatccacg ccctgggtgt aacgcatcca gccagccacg cccagcgcca ggtgacgcca
     1321 gctaccgccg ttttgcagat gcagacgcac cggatccagc agacgctgcg gtaacttctg
     1381 gctgccgtcc atcgcaatct gccaggtacg gtgacgcaga gacgggttac tgaaacgctc
     1441 gatcagcaga gtcgcatagg cgttcaggtc tgtaccttcc ggcatcgaca gcgttggcgc
     1501 ttgttcctgc atcatcaggg caaaggccgc tttgcgataa tccggattag tcatggtgtc
     1561 ggcaatagtt tcatagccgc cgaggtaacc gaggtacgcc aggaaagagt ggctgccgtt
     1621 cagcatacgc agtttcatca tttcgaacgg cacaacgtct gcaacgaact gtgcgcccac
     1681 tttatcccag tccggacgac cattaacgaa gttatcttca atcacccact gacggaacgg
     1741 ttcgcaggca atggcgcacg ggtcgtaaac acccagctgg tcagcaattt cctgtaaggt
     1801 ttctggcgtc gcagccggaa cgatgcggtc aaccatggtg cacgggaagg tgacgttttc
     1861 ttcaatccat gccgccagtt gcgggtcacg cgcctgagcc agcccaagaa cggcaacctt
     1921 cgccacatga ccgttttcac gcacgttgtc gcaggacatc accgtaaatg ctttcagccc
     1981 tttttcacga cgcagacgca gggcttctac gatgtaacca atcgcagact tcggcgcagt
     2041 cgggttttcg agatcgtgct tgatcagcgg gttattgaga tccaactgac cgcttgccgc
     2101 atcggcacag tagccttttt ccgtgaccgt cagagagaca atcgccgtct gcggacgcgc
     2161 catcgcgttg agaatacctt cgcaaccgtc gatttccgga tgcagcgctt ctttcatcga
     2221 accgataatt ttcagctcgg tgctttccgc gcctttttcc gccacggtgt acagcagttg
     2281 ctgttttttc aggttttcga tcagcacgcg gtcgttgcct ggcatcaggt taacttcgca
     2341 gataccccag tcgctgtcgg tactttccag cagatgatgg gtatacagcg cctggtgcgc
     2401 gcggtgaaac gccccgcaac cgagatgcac aatgcgtgat tccagacgag aatgatccca
     2461 tgacgggcgg gcaaccggca gattgctgtc aacaatagta gtcatcgtaa actccagaat
     2521 ccgtcttcag agagtgacgg caaggaagaa ggaaccggca cggcgcgcag ccatgccggt
     2581 ggatattaac ggctaaagaa agcgcgctgg atcgccagtt cgacaccgcg aacttcggcc
     2641 aggcctttca gacgaccaat tgcggagtaa ccaggattgg ttttcttctt caggtcgtcc
     2701 agcatctgat gaccgtggtc cggacgcatc gggatcaggt cttctttgcc ttccgcttta
     2761 cgacggtgtt cttcttcaac aatcgctttc accacttcgt acatatcaac gtcaccgttc
     2821 aggtgcgccg cttcgtggaa ggttttcggg ttatcttcac gcatggtgga gcgcagatgg
     2881 gtgaagtaaa tacgcggacc gaactgcttg atcatatcaa ccagatcgtt gtcagcacgc
     2941 acgccgtagg aaccggtgca catggtgaaa ccgttcgcca tgctgtttac ggtatcaacc
     3001 atccactgca tatcttcaat ggtggaaaca atgcgcggca ggccgaggat cgggcgcggc
     3061 ggatcgtccg ggtgaaccgc catacgcacg ccaacttctt cagcaactgg aataatcgct
     3121 ttcaggaaga cagcaaagtt ttcgcgcagt ttggctttgt cgatatcttt gtacagctcc
     3181 aggtgcttac ggaactggtc gagggtatac ccttcttccg cacctggcag accggcaatg
     3241 atgttacggg tcagacgcgc tttgtcttcg tcgctcatgg tggcgaagcg aacagctgcc
     3301 tgagcaattt cttcttcggt gtaatccgct tccgcgcctg gacgcttcag gatatgcatt
     3361 tcgaatgcag cgaattcgat ctggtcgaag cgcagagctt tggagccgtc tggcagcacg
     3421 tattcgaggt cagtacgggt ccagtcgagc accggcatga agttgtagca cacggtgcga
     3481 atgccgcact gcgccaggtt gcgcagggtc tgctgatagt tagcaatcca ctgctcatag
     3541 ttgccagtgt gggttttgat atcttcgtga attggtacgc tttcaacgac agaccacacc
     3601 aggcctgcgt cttcaacgat cgccttgcgt ttgaggatct cttctacgga ccatacttcg
     3661 ccgttcggga tatggtgcag cgcggtaacc acgccagttg cgcccgcctg acggacatca
     3721 gctaaagaaa ccggatcgtt tgggccgtac cagcgccagg tctgttccat gatttttcct
     3781 ctttcacata gtgggttcca cagcaaagag acgtggagcc ccggaattgt ttaggtatcc
     3841 ggatgaacaa ccagaccgca gccgtggcgt tgcatcggat gagatggcgt ataagttcta
     3901 ccttttggta agacatgtca acccgaaatc tgaaattggt taaccacatc acaagaattt
     3961 cactctcctg gttgttgctg caaattgcag caaagggtaa tcgatggtta ttgatcagag
     4021 gatgtgacat tcatcgcaac aatggttgac caatttacat aacatgtcgc ccaaaataac
     4081 aacggttcaa ccaggtaagg taaaacgtta tgcatgtgct taacattctc tgggtggtat
     4141 tcggcattgg cctgatgctg gtactgaatt tgaagttcaa aatcaattcg atggtggctt
     4201 tgttggtggc ggcgctgtcc gtcgggatgc tggccggtat ggatttgatg tcgctgctgc
     4261 acaccatgaa agcaggtttc ggcaacacgc tgggggaact ggcgatcatc gtggtgtttg
     4321 gtgcggtcat cggtaaattg atggtcgact ccggcgctgc tcaccagata gcgcataccc
     4381 tgctggcacg tctcggcctg cgctatgttc agatatcagt gattatcatc ggacttattt
     4441 ttggtctggc gatgttctat gaagtggcct ttattatgct ggcgccgctg gttattgtta
     4501 ttgccgccga agctaaaatt ccgttcctga aactggcgat cccggcagta gcagctgcca
     4561 ctaccgcaca ttcactgttc ccaccgcagc cgggtccggt ggcgctggtg aatgcttatg
     4621 gcgcggatat ggggatggtt tatatctatg gcgtactggt gacgatccca agtgtcatct
     4681 gcgcaggtct gatcctgccg aagttcctcg gcaatcttga gcgcccaacg ccatcattcc
     4741 tgaaagcaga tcaaccggta gatatgaata atctgccctc tttcggcgtt tcgattctgg
     4801 tgccgctgat cccggcgatc attatgatct ctaccaccat cgccaatatc tggctggtaa
     4861 aagatacccc tgcctgggaa gtggttaact ttatcggttc ctcgccgatt gcaatgttta
     4921 ttgcgatggt ggttgcattc gtactctttg gcaccgcgcg tggtcatgac atgcagtggg
     4981 tgatgaacgc ttttgaaagc gcggtgaaaa gcattgcaat ggtgattctg atcatcggtg
     5041 cgggtggcgt gctgaagcag accatcatcg acaccggcat tggcgacacc atcggcatgt
     5101 tgatgtccca cggcaatatc tcgccctaca tcatggcatg gctgatcacc gtactgattc
     5161 gtctggcgac gggtcagggt gtcgtttcgg cgatgaccgc tgccgggatt atcagtgctg
     5221 caatccttga tccagcaacc ggtcagctgg ttggcgtgaa tccggcgctg ctggtgctgg
     5281 cgacggctgc gggttccaac accctcacgc acattaacga tgcatctttc tggttgttca
     5341 aaggttactt tgacctgtca gtaaaagaca cgttgaaaac ctggggtctg ctggagctgg
     5401 tcaactccgt ggttgggctg attattgtgt tgattattag catggtagcg taaaagaaca
     5461 ccggagcctg ccaatggcag gctcagttcg gattgtcggc aaagtgcgct ttgtttatgc
     5521 cagatgcgac gctgacgcgt cttatctggc ctacaaaggg ctaacgtgca ggtttttagc
     5581 ttcaggtaat attgcgtaac tgcattagca atgtcctgtg atttctttat tgataaacaa
     5641 aagtcacgcc aataatcgat tgcacattcc ctgcagtcac ctgcccaccg gtacgtgcgt
     5701 aatttgccgt taatcccaga cttaccgccg aagtccctac tgctcctaac gataccgtgt
     5761 tattcgctgg aataatcgta ccgttgcgcg tcaactgtac gccgacgccc tgtgctggtg
     5821 aaaacgaggc ggtattggtg aaaatcgagt tgcccgcatc tgcggttgtg ccggagaggt
     5881 aataccccag gttttggctt ttcgcacaat aaacggtaag aggaatcggc accgaaccgg
     5941 gatagtccgg cagagtaacg gtgacatcac gagcagaaac atcacagccg ccagtaggca
     6001 ccaccacatc attattggcg taaatattcc acacaaactg gaaatcatcg ctgttatagt
     6061 tgttggtctg tcgcaaaatt agcacggcaa ttaatgagcc agctttaatc gccaccccgc
     6121 ccgcactgct cacaggcgtc aaataaagcg ccaccggcca cggcttatcc gttctcgaat
     6181 tataaacaac gcgcggcgtt tcgctggtgg tcggaaatgg atagctactg ccactatatt
     6241 ttacggtccc ggaaaaatta gataacacgc cgccataagc cgagcctcgt tgcagtgtga
     6301 catagtctgt aatggtttcc ggataatcgt tatggcaaaa gatttgcgtc gaaagatcca
     6361 cgaccaggtt ttgccccaca ttcacggcgg gcgcaaggtt tacataaaca ttggcgctgc
     6421 caccgccaat agggatagcg gtgccattgg cggttttaca agcgaatgac caggcattta
     6481 ccgaccagcc catcagcagt acagcaaaca gggtaataac tcgtttcatt acaatcatct
     6541 cttcggcttc agctgtaggt ataggtgatg ctaattactg cctgaatggt tccctgagtg
     6601 gctccgccat ttaccgtcaa tgctctgacc tgtaacggga agtgcgctga ttgtgaggaa
     6661 tcatccacct gaactgtttt ggttgcgcca ctattcaatg tgttgccact gtcatcctgt
     6721 agctctaact ggatgttttg cgcggtcccc tggtttttat aatatccggt actgtcggct
     6781 gccccgctga agctggcagt gaccctcgac gttcccaccg gacaattagt caactcaagc
     6841 gcaacatcat gccaggccga tgccgccccg gcagacatca gactgaaaga ataaagatcg
     6901 ccgagatcta ccgtggcatt ggtggtggaa accgtgcacg gtttggcgac gaccttaccg
     6961 ttcaccgtga tggtgacatc ggctgcctgt atcgtcgcac ttgcgagcgc caacattgcc
     7021 gccaataaat acccacgttt gcgccatttc atgagcatct ccagttactg atattcaaga
     7081 gtgaaggtag ccgtggcatt gatatgcccc gcagtgacag gcacctgtgt cgccattagc
     7141 cgggcgtaaa aattcagcgt atttggttta cccggcgtca gggtcgtcca cgaaatcgcg
     7201 gacgatgggg cattaagggg tatctgattt tgttgctcat tcagaagctg tattcccagt
     7261 cccgcagccg ctgacaccgt attttcaagt gcaagcaggt tggcattgtg gctatctgca
     7321 acaccggtaa acccaacttt tacggcagaa acggcattac cacagggtga cagcaaaata
     7381 cgaaatggaa cgacaggagt cgtcgcgcca atgttgttaa attgcttcgc cgcgttttcc
     7441 atcagatcaa cagtaaaatt ggttgattca gcggccacac tacagccgtt atctctgaca
     7501 tagccgcgga tagtaatcgt gctatccgca gccaaagcgt gactcaccgc cagccacaaa
     7561 aaagcgcaca gaagataaaa aggtttgttt ctcatcacgc ccccttaacg acattcagct
     7621 gatagctggg ttaataactg ctgctgactc tctggtggca gttgataatt ggcgacacag
     7681 tgagcatttt cctcttctcc ccatttcacc tgaacttttc ccgctaaagg cattccgctg
     7741 aggtaaacct gaccattatc cgcaacaatg ccgctactct ggctactctc tgatgtcacc
     7801 atcgccccaa acggcagcgg cttattattg tgggttagcg tcatgagcag ttttatccca
     7861 acgcgcgctt taaactctgc tcgcacgatc gccccacggg tgggaacaac gttagcgacc
     7921 gcgttatcta aatcgacgtt atcagccagg gtattggtat ccagcgctac tctgttttcc
     7981 cgatattcag tggcataagg cagcacggca taaccgcgcc agtcggtacg cacccccgtc
     8041 tggttttcga cttttgcatc ttttgcgcca ggcgctttaa caagcaccac cgtatcgttt
     8101 aacggctgcc ccagcgttac gccattggca tgagccagta ccccaccgct gactccgtaa
     8161 tagagctgct taatatcatc gctatggctg taaccgatgt tggcattgcc gtaaccaccg
     8221 cgataattca gcgtggcgta gcctgtactt ccgctattac catcacctcc cccggcgtaa
     8281 ccggtttgca cgctatagct gaggttgttg tcttccagca acgtaccgta tacaccggcc
     8341 agattggtca tccgaccgtt gagatcgtgt gacatgctgt aactggcact ggcatgtcgc
     8401 cactgagatt tactgtcaga acgcagccag tggctgaaag gaatattgac gttaagcgct
     8461 aacatctgat cccgtccttt ttgccaggcg tttttcgtca ggctatagct gagcgtccag
     8521 ttgatatctt cgaatgcagt atttaaacca gcctggaatt gctcatcgac attattcgtt
     8581 ccccaataag tttgatggct accactcaaa tacagtgttg atgtgcgccc gagttgctga
     8641 gtaacggtga gctgtaattt cccgcgtttg ttataagcga gattgtaata gtcggtgaat
     8701 ttcggcttaa cctgaataac tccatcctgt gtttcgatgt tgtagccatt cattcgactg
     8761 tatgttgtgt cagcgaaatt aaaataccca ctggtcgaat aacggtaacc cactaactga
     8821 atattcgtgc ctgactcatt gagcgatttg ttataaagaa aacgcaccga ttgtccgtca
     8881 tgctgactgt catcgggaag tgtggaatta gcctgcgtca tatccacaga cagagcgccc
     8941 agtgccccca tgtttttccc gataccgaaa ttaaacgcac gataacgatc cgccagttgc
     9001 gttccaccat atattgtcca gccagccgga aggccgtgga gtaatgtact ctggaaaaag
     9061 cggggttttt cctgctgcgc atttccacta cggtattctc ctgccgtaat ggaataacgt
     9121 gtatgccctt cacgttgcaa aagcgggact gacgaatagg gtacggtaaa gatctgcgtg
     9181 ctgccgtcag cctctttaat tgttacctgc aagtcaccac tattacctgc ggcatagata
     9241 tcgttgatgg taaaaggccc cggcggcacc gtactattat aaatgtcata cccattttgt
     9301 ttaatagtga cctgtgcagt accacgagca ataccgtgga tcaccggggc aaatcctctt
     9361 tggctatcgg gtaacatatt gtcatctgag gccaattgtg cgccgcgaaa gttaatacca
     9421 tcgaaaatat cgccctgggt ataaccatca cccagcgtca gccgggaacg taacggaatt
     9481 atgtctcgct caagccaggt attgatatgt tgccatttat ttttgctacc tgatgaactg
     9541 tcgctactgt tataactcca ggtggtattg tcgcgtaaac gccacgcacc aatatttagc
     9601 ccactctgta ggtttaaata tgcataatgg ctgttactac caatccgatt ctgtacacta
     9661 tttccgctga aattataatt gagtaagccg gcattaatac cgggatccca caactcagga
     9721 ggaatatatc cacgcgcgcg attactcata aatgcctgag ggatcgtcag gttgagtcgc
     9781 tgctgaccaa catctaaatg cgcagtagcg tcatggatca ttgaggttaa tggcacgcag
     9841 gcatcatccg ccagcagatt cataccggag acagaagccg tattcagccc catactggca
     9901 agttgcgcgc gtgtcaggca gggaacaatc ccttgttcac tgtcgcccgt attaaatgtg
     9961 acatcacgcg ttgccatata accattattc aaatagatat cgacacgata cgtccctggc
    10021 ggtaattctt gcccattctc aaaacgcgat aaatcagcca cagcctgggg atcatccgct
    10081 aaaaaacgcg ggttaaaata gagttcggca gatgacaaag gtgcctgtac ggcaaaagca
    10141 caggcaacaa agagccggac aaaaaaacca gccaaacgat gcttacgaat atgcaagcat
    10201 tgtgtgtttc gctggtaaag tcttaaattc agatatgaca ttattcctcc ggtttaaaat
    10261 cacccggcag tcaattcttt ttattcaggc gaaaaatccc ccctgcgtta ttccattacg
    10321 cccgtcattt tgggggtaag tgcgccataa tcatttattg ttcggtaagt aatattgctt
    10381 cctgcatcag aaggcaattt aaccgtgctt tcgcccattg gaggcaccaa tgcattttca
    10441 agaacccggg ttccggcatt caactctgtt accgtcaggt aatagggtgt cgggttaatc
    10501 agcgtcagag aattcgcgct acgacgaaat cttaattttt ctgcggcctg atcgggtggc
    10561 aacgctaatt tagccgggcg atagtacagt ttaatgcggc tgataattgc gagctgtagc
    10621 gtattctcag tcaattttga tttatccatt gacggaatcg ctttaacgtt catccagaat
    10681 aaactttccc gatcctgtgg taattggtta tttgttgcat caagaatacg taaggtattc
    10741 tctttttttc ccttcatcgc aaacagaggt ggcgtcacga taaaacgacc atcctttaca
    10801 ccatcggcat tttccaccca tgattgaatt aaataggtgc tattttcatc attatttgtc
    10861 acggcaagtt gcacttgttt ttgccccgcc ggataaatta cgcgggtcgc acctaaggcc
    10921 actcccgctt cagcgcgtcc ggcaaccatc attgccatga acatcaggat acctgccagc
    10981 aagcagaatg ttatttcctg cgatttcctt acattgacgt ttttattact cactgtcctg
    11041 ttcctgttat cacattatct gctgaacaat tactgatagg ttaaagagaa ccaggcctgg
    11101 gcattggcga tgccgccagt aacccgacgc ccggtagcac gatatttggc gatgaaatgt
    11161 agcgaagtag agcctgagta aagccgtttc cagtttgctg gaggacgatt aatcggtacg
    11221 aggtttcctt catcatcaaa taacgctacg ccaatattgg tggctatccc tggcccctct
    11281 cccacggaaa gcacatccgg atttttacca tccgcgacac cgtgaaacgc cacacccaca
    11341 cgttcactca ccaccgtgct acattcccgt aaatgaataa caaaaggcac cggtgcgcta
    11401 tcttccccta ccgcatgaaa ccggttactg ctgatttgcc ccatattgac cgtcatttgt
    11461 ttatcgccag cttcaatccg gcaagtttcc gcaataatga cgccctgaaa ttgcatattt
    11521 ccgccgggca gcgtggtatt ccatttattt ccggccagag caaacattgg cagcaacgac
    11581 gctaacagaa ataatctctt ccttttcatc gttattttta tcgcacaagg gtgggcatcc
    11641 ctgcccgtaa tgacgtccct gaacctgggt aggttattga tactgaacct tgaaggtcgc
    11701 atccgcatta gcagcacctg gggttgcggc accggttgca aaataacgcg cctggaacgg
    11761 aatggtgttg gttccgttat tcagggttgt ttctgaacta aatgtcgcac cgtccagcgc
    11821 cagcgcagca cccgttctgt ccaggatctg cacaccaacg tttgttgcgc tacccgcagc
    11881 tgaactctgc agagccagta catcagtatg cgtagcatca attgccgtac ctaagaaggc
    11941 aacagcggct tttgtggcaa cagtggtatc gcaatcattc agctgaatgt taaaaccaac
    12001 ggcagagctg gttgctccag cctgcttcag gctagcggta cgaacctggc ccaactgaac
    12061 ggtttgatca acagagcctg catcaactgc gcaagcggcg ttaacaactt cccctttaaa
    12121 gtgaacggtc ccaccattta ccgtcgtagt atcggccaga gccgctgcgg aactgaggga
    12181 cagagccgac agaacaacga ttgccagagt tttaattttc atgctgcttt cctttaaaaa
    12241 aactatttct aaatcgacat gggcagtcgt tctgtacact ttgttttgtc aacgagtttg
    12301 actgccaaca ctgcacagtt ttcccccaaa agatgaaaca tttggggcca ttttgactca
    12361 tagaggaaag catcgcgcac aaactttttc agtttatttg ttggcttaat attctattgt
    12421 tatctttatt tatagatttt tatattgcat gaggtggttt ttggagagaa gaatgaggaa
    12481 gatgcgtcga gccacagaaa cgttagcttt acatatagcg gaggtgatgt gaaattaatt
    12541 tgcaagagaa ataatttaca tatcaaacag ttagatgctt tttgtcgttt tttaatattt
    12601 ttatgcttga gaaaaaatac gtaacttatt tatgatatgg acagttttgg ccccaattgt
    12661 cttgtattta tttgtttttt aactttatta tcaatgagtt aaatcaaatc tcttctcttt
    12721 ttaatttttc gtttatgaga ttatttcttt cccataatcc ggcaaaacga gcagcattac
    12781 tggcggtata acgtacagta tggcgaatat ttcgatgccc gagataatcc tgaattaaac
    12841 gggtatcggc accacgctcc gccagttcat aaccgcaagc atgccttaac atatgaggat
    12901 gagtctgcgt tacggttcca gcttcaatac ctgcatcgcg aataatgcga taggcctgct
    12961 ggcgagaaag ccgactcccg cggcgagaaa taaatatagc gtcggtccgg tcagcgcctt
    13021 tccagttagc acgttcctgg gtccagcgtt ccacggcttc acgctcatca aaacgtaacg
    13081 ggtgaacggt agaaaatccg ttcttcagtc ggcgaatatt tattcgacct tcattaaggt
    13141 caagatcctg ataatgcaga tcaagcagtt cactaatacg catcccatgc cgatatgcca
    13201 acagaataag acaataatct ctggctcccg ttgccccgta acaaaccgcc tgcatcatgg
    13261 cctgaacttc tttaccggta agataacgac gtttactcac aatagtagta ctcctgactg
    13321 agatatattt gaatgaatac ctataggaaa cctcaatcgg tcaaaattag cccaatagat
    13381 aaaaagataa ccacacacga agtgatgtgg ctat
//
LOCUS       DAPHCV010000120        13192 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.121, whole genome shotgun sequence.
ACCESSION   DAPHCV010000120 DAPHCV010000000
VERSION     DAPHCV010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.121"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1936)
                     /locus_tag="RH033_003818"
     CDS             complement(<1..1936)
                     /locus_tag="RH033_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992940.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALTSTASALAGFVLLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRSIQQNGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKRLTSAIRQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLTLISQRISRGELREFELADGNWLLDNAWYERNMAGFNEKLRES
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQDIYERSYTGFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNHGPMQLEVPRDQYAGAVETMKNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLTVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKVM"
     gene            complement(2057..5179)
                     /gene="ag43"
                     /locus_tag="RH033_003819"
     CDS             complement(2057..5179)
                     /gene="ag43"
                     /locus_tag="RH033_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HDV2992941.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARARGKRTGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTGGQ
                     WIKAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWVH
                     GGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTIN
                     KNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHKYGLALNTVINEGGW
                     QVIKEGGTAAHTTINQKGKLQVNAGGEASDVTQNTGGALVTSTAATVTGTNRLGAFSV
                     VAGKADNVVLENGGRLDVLSGHTATNTRVDDGGTLDVRNGGAATTVSMGNGGVLLADS
                     GAAVSGTRSDGTAFRIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGSLA
                     GTTTLNNGATLTLSGKTVNNDTLTIREGDALLQGGALTGNGRVEKSGSGTLTVSNTTL
                     TQKTVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLTSGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSARTGKFVPATLQVKNLNGQNGTISLRVRPDMAQNN
                     ADRLVIDGGRATGKTILNLVNAGNSGTGLATTGKGIQVVEAINGATTEEGAFVQGNML
                     QAGAFNYTLNRDSDESWYLRSEERYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVNG
                     ENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSLTTGVY
                     GAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYMNLTHTSSGLWADIVAQGTRHSMKASS
                     GNNDFRARGWGWLGSLETGLPFSITDNLMLEPRLQYTWQGLSLDDGKDNAGYVKFGHG
                     SAQHVRAGFRLGSHNDMTFGEGTSSRAPLRDSAKHSVRELPVNWWVQPSVIRTFSSRG
                     DMRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYAHSINGSSAEGY
                     NSQATLNVTF"
     gene            complement(5513..6385)
                     /locus_tag="RH033_003820"
     CDS             complement(5513..6385)
                     /locus_tag="RH033_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HDV2992942.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILLCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
     gene            7726..9216
                     /locus_tag="RH033_003821"
     CDS             7726..9216
                     /locus_tag="RH033_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HDV2992943.1"
                     /translation="MPYFQYPDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFLLTLAESGERKTAVDKLLMEPLYQQEMLLYSRHK
                     NELTSWKNKEEILKAQKKALLSKLNKELRKGADESETLRQLEALQKNREEKPVRYKFI
                     FNDATTAAIKDQLCGQWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VTPGGSLTAFHERLMELARGSIEKSSKDERYCLHFSPEAQKIFIDHYNVLEQDLSPSG
                     PLSQFRGHVSKKTENIARIAALLQYFSQGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQLQDARELLDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNVLLNILAS
                     QLRLSVVQEGKTTYVLLSEINNITLDKLNACYSQKLIRWHEQSFGL"
     gene            complement(9338..9910)
                     /locus_tag="RH033_003822"
     CDS             complement(9338..9910)
                     /locus_tag="RH033_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992944.1"
                     /translation="MQKLENRCELLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGTIISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(9996..10202)
                     /locus_tag="RH033_003823"
     CDS             complement(9996..10202)
                     /locus_tag="RH033_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDV2992945.1"
                     /translation="MNTPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVETWLQARIAQSRP"
     gene            11059..11283
                     /gene="hha"
                     /locus_tag="RH033_003824"
     CDS             11059..11283
                     /gene="hha"
                     /locus_tag="RH033_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HDV2992946.1"
                     /translation="MQHKVTRDEWKLKPEQFRRCSCRETLEKVSSHTRYKLTPAELEA
                     FNSAVDHRLAELTMNKLYDRVPASVWKYVT"
     gene            11989..12558
                     /locus_tag="RH033_003825"
     CDS             11989..12558
                     /locus_tag="RH033_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HDV2992947.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGELKGKKHYHLLLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDAGRYDTLAHFPDKPAVWLERDDETGFQ
                     QVLERAGYLAKEHTKARGTGERNFGCSRG"
     gene            12724..13107
                     /locus_tag="RH033_003826"
     CDS             12724..13107
                     /locus_tag="RH033_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992948.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGERYCTHCQTGRFSEEGVAE"
BASE COUNT         3113 a         3365 c         3478 g         3236 t
ORIGIN      
        1 tcatcacttt gttcagggca ctgatatttc ctgcaccggt ccccttctga agcgcctgcc
       61 ggacagtcgg gctggccgtc gaaaaatcaa tatgcttcag cataccctga acaacagtga
      121 gccggtgaag ttgctgggtt gtgacgtaga cagccagtgt gcgggtgaag gtttttcctg
      181 cctggacagc agctgtctgc agggcagcat cgcgatcgcc ggtgctgagc cagaagaccg
      241 atgccgtcag ggcaaaactg atccctccgg ccgccagact gacaaccgac ccctcggcaa
      301 tatcataagt gaccgattcg atggtcccga accgggtgat attacgggcc tgggtataag
      361 tcagatgtcc ccgacgaatc agccgggacg cttctgcggg atcggttaca ccgggtactt
      421 taccttcgcg gatcttattc ttcatggttt ccacagcgcc ggcatactga tcacggggga
      481 cttccagttg catgggacca tgatttccca tgtaacgata ctgtccgttt tgtccgtcga
      541 atgccgcacc gacgctacgg gctgcagttg aacaatattt cgtctggatt tctgttccgc
      601 tgaccagtct gtctgctcca tttctggcat tattatcacc gaggatccgt gcgtttttca
      661 gacggactct gtcgacaata tggtttcccc gttcagcggc aaacccatgt ccctgccctc
      721 cggaagcaaa cattttctca tttgtactcc aggtttccgg cagtttcatg agaatcagac
      781 cattcgccgt tgcatcggaa ataaaaccgg tgtaagaacg ttcataaatg tcctgacaca
      841 gtgtttctgt ttcgcgacaa agagaggagc agtaccattc cggatccggc ttatgatttt
      901 ccggggcatg atgtacttct gtcagcccgc aggaggcaca gatacgaagc tcatccacgt
      961 ggtggaatga tacaaaacgg cggccttctg aaaaggggta ccagtcaccg ccgtccaggc
     1021 aacgatcgag aaaatcgtca ttcgcttccg gtgataaatt caggcggttc cggaagaggg
     1081 ttttcagttc atcaggggta aatgacaggc tctctctcag cttttcgttg aaacccgcca
     1141 tgtttctttc ataccaggca ttgtccagta gccagtttcc gtctgccagt tcaaattccc
     1201 tcagttctcc ccgggaaatc cgttgtgaaa tgagtgtcag ccagatgcgc tccgtcagcg
     1261 tttcaggaag gctgtaatcg ttttgcaaac tgctcagcca cgatttactg ttgagcaccg
     1321 gttgttcttc cagtgtctgg cggatggcac tggtcagtct tttcagcgcc tggatctggc
     1381 tggccgccca tagcagcgtc actaatgcag ccagccagta caacaacccg ccccaccaca
     1441 tcagtgaggg tgaaatatcg aactgcaatg cgatcaatgg tgcgtatgca gaacacgcca
     1501 taaggaaaaa agcagtataa aaacagcagt aatacccgtt aatttccttg ctgatatctt
     1561 caggtgtgag cccgttctgc tgaatgcttc ttatctgcac gggaagagcc agcagaccag
     1621 cggcgagagc ggtggtcaga ataaatttta cagagtcaaa agtatgagcc tccagtgcga
     1681 tatgtttacc atcaagtccc cacatgaagc tgatgatcat tccggcaatc gtcagaaata
     1741 cgcctgccag ccagcgaata tggaataacc gggttatgcg ttcagtgata tagccagcca
     1801 gtaaagccgg gccgaacaga cacagcaata cgaatcctgc cagcgcagac gccgtgctgg
     1861 ttaatgctct gaacaaaagg cgaagaatgg caaatcctgc gatcagcagg atcagcgcgc
     1921 cgactatctg taacatagtc actccgtgat gaatatcatg ttattgtgaa tgccagtgaa
     1981 tgtggcactg aaacgttttc ccccgtccgg gtcccggtca tgatgaccgg gaccacagag
     2041 aggcaatgca gaattatcag aaggtcacat tcagcgtggc ctgactgtta taaccttcag
     2101 cgctgctgcc gttgatgctg tgggcataac cggcctgaac gcccagggtg atattttccc
     2161 ggacacgggc ttccagtccg gcctgcagat ccagtgaagt gccattctgt gagggagaga
     2221 acgtcatccc gctgcctgcg gtggaggtcc ccacacgcat atcaccccgg gagctgaagg
     2281 tgcggataac ggaaggctgt acccaccagt tcaccggcag ttcacgcaca ctgtgttttg
     2341 cactgtcacg cagtggggca cgggatgagg tgccttcacc aaaggtcata tcgttgtggc
     2401 tgcccagacg gaaaccggca cgcacatgct gtgcactgcc atgcccgaac ttcacataac
     2461 cggcgttgtc cttaccgtca tccagcgaaa gcccctgcca ggtgtactgc agccgtggct
     2521 ccagcatcag gttgtcagtg atactgaagg gcagaccggt ttccagtgag cccagccacc
     2581 cccagccccg tgcgcggaag tcgttattgc ccgatgacgc tttcatactg tggcgggttc
     2641 cctgtgccac aatgtcagcc cacaggccgg aggaggtgtg tgtcaggttc atatatccgc
     2701 ccaggctgcc ggcatcatcc cggaccgtgc cggcgcggga gccgtcatca tccttaacat
     2761 caacggaaga atggcctgca gcaccataca ctccagtcgt cagtgacata ccggcgacct
     2821 ctgttctgag caggtcaccc tccagacgga cgaagccata gctgccgctg ctttccggcg
     2881 tggccccacg ggcaataccg ccgttgttat cgtgaccgag atggccgccc tgaatgctga
     2941 gacggacgct gttattttca ccgtttacac cggtctgatg gctgcgggag cctgccagaa
     3001 tccggtcata gtccattgcc tgtgtcagca tggaggcgta cagggggact tcagcacgat
     3061 aacgttcttc actgcgcaga taccagctct catcactgtc ccggttgagg gtgtagttaa
     3121 aggccccggc ctgcagcata ttcccctgga caaaagcccc ttcctccgtg gtggcaccgt
     3181 taatggcctc aaccacctga atacccttac cggtggtcgc cagccccgta ccactgttgc
     3241 cggcgttcac cagattcagg atggtttttc cggttgccct gccaccgtca atgaccagtc
     3301 tgtcagcatt gttctgcgcc atatccgggc gtacacgcag gctgatggtg ccattctgcc
     3361 cgttcaggtt tttcacctgc agggttgccg gtacgaactt ccctgtgcgg gcggaggtga
     3421 aatgaatctg tccggcatgg ctgaggtcat ccaccaccga ctgaaccgtg gcgttatcgg
     3481 ggatattcca ggtggcaccg gatgtcaggg tgacattcgt ggggtcaatg gcaccgttca
     3541 gcactgtgct gccggtcagc ttcagggccg tgccgcgctg agcgatgaca tccgtggtga
     3601 cggtactgtc gtttagcgtc agcgtgcctt cattcaggtt gacggttttc tgggtgagtg
     3661 tggtgttgct gacagtgagt gtgccacttc cggatttttc caccctgccg ttaccggtaa
     3721 gagcgcctcc ctgcaggagt gcatcacctt cacggatggt cagggtatcg ttattcaccg
     3781 ttttcccgga aagggtaagc gtggcaccgt tattcagcgt ggtggtgccc gccagcgagc
     3841 cccctctggc ggtgaacagt ccgccattta ccgtggtatc cgtggccgta tcaccggcgt
     3901 tcagtgtgaa ggaactgcct ttttccagca tcagggcatc cgcctgaccg cctccgatac
     3961 ggaatgccgt tccgtcgctc cgggtaccac tgacagcggc accggaatcg gccagcagta
     4021 ccccgccatt ccccatggat acggtggtgg cggcaccacc gttgcggaca tccagcgttc
     4081 cgccatcatc cacacgggta ttagtggctg tgtgtccgct cagcacatcc agacgtccgc
     4141 cattttccag tacgacatta tcagccttac ccgccacaac ggagaatgct cccaggcggt
     4201 ttgtaccggt gacggttgca gctgtactgg taaccagtgc accgcccgtg ttctgggtga
     4261 catcagacgc ttccccgccg gcattcacct gcagctttcc tttctggttg atggtggtat
     4321 gcgcggcagt tcctccttcc ttaatcacct gccagccccc ctcgttaatt accgtgttca
     4381 gcgcaagtcc gtatttgtgc acatactggt ttcctccctc cagccgggtg ttatgtgcct
     4441 ctccgtgtac cgtctggtca ccgccggcgt aaatgagggt gtcccgtgcc atccccgaag
     4501 accagataac ctgccggcca tttttgttga tggtggtgga ttcagccgtc cctccgacca
     4561 tctgaccgct ggacacattt tcagactccg gaccaccttc tgcaccggtg ttgacgatgg
     4621 ttccggttgc cagtccgcca tgttttatgg tctggtaacc atcctggtta ataattgtac
     4681 cggcagcttt ccctccaccg tgtacccact gctcacctct gttatccagc gtggtgttca
     4741 ccgccagtcc gttaaggctc tgccctccgc catcacgaat aaccgtatca ctggcagttc
     4801 ctcctgcctg cacaatctga cgaccatttg cggtgacagt ggtgtttctg cctgtgccac
     4861 ccgcttttat ccattgcccg ccggtgtttt cgtcactgtc cggccccagc tcaagcccgg
     4921 tactgacagt cacgccatca gctgttccgg atacaaactg gttgtcatgg tttaccagtg
     4981 ttccgccatt cactgtttca cccgggtgca caacgatgtc agcagccagc accgggagtg
     5041 acgtgaccgc ggcaagagac agtgcaaccg ccacaccggt gcgtttcccc cgtgcgcggg
     5101 ccagttcgga ggcaaccacg aaagcgcccg taatgtgatt ccataccagc ctgtagcagg
     5161 tattcagatg tcgtttcatc agcatttcct taatcagagt gagggtgaat aaaaaagccg
     5221 gtatccacag atgtggatac cggcaggtaa agggttacag tgccggaggt ccggctgacg
     5281 gcagtttctg acgtgtggct ctgttgttcc ctgaatctca tgtctgtgtg tatctgcgca
     5341 cgttgctaat aggagttatt attagcataa cgatcgatat taacgatcgg tgaaataggt
     5401 atcgatcgtt ttattctatt atccggctat tgcacggaca agagagacag taacgaaaac
     5461 agcgcttttg tgagcagact cagaggcagg gaggagatca tccacaacag gttcagaaga
     5521 agatccagtt ccataccgcg cgggcaacag agaccacggt gtcacgaaca gcgcgcaggg
     5581 ccgtacggac aacagaggca acacagacgc tctccgctgt gtcaaatatc cggtccaccg
     5641 caccggtaaa ctgttcacgg gcctgcgagc ggacagactc cgtgcgcagc tcgtcctgca
     5701 ggcgggtcat caggggactg gcggcatggt cgggaagtgc tgtcatgagc gcactgacca
     5761 gcgtatccag ttcccagccg gtgcgggccg atacggccac aaccggatgt acgggccgga
     5821 acagccggaa taccgcctct gttttctcac gaatgttctg ttcctgtgcg ggagaaggct
     5881 gaataccggc catatcccat tcatggcagg gttccgtttt gtcggcctgc gtcaccacaa
     5941 acagcacctg ctgatgtccg catagcagga tgtgtcgcca gaaatactca tccacagaca
     6001 gggcacggtc atcggcttta atcagccaca gtaccaggtc cagttcaggc agaatgtcac
     6061 ggtacagggc ttcatactct gcatccctgt cccggctctc gcccaccccg ggcaggtcag
     6121 tgataaccat gctgtgacca tggccactca gacggaagcg ctgcacttcc cgggtgccgg
     6181 cgtgaacatc actgaccggg gtgacctccc cctgaaacag tgcattacag agtgaggatt
     6241 taccggcccc gcttttaccc ataatgccaa tcacgggttc gtgacgggtg agtttacgca
     6301 gatgttccag gatgtgacgg gaaagtgagt aaggcaggga ggaaagcggt ttttcaattg
     6361 cctcaatggc atcagacgga ttcatacatg tattcccggt gaaaaaagac aaaaccccgc
     6421 gcaccggaaa ggtggcgggg gatccacgga gataatatct gtctgaaatt gttttgaatc
     6481 gatgggattg ttgcgtatta acatgaagga gatcaggact tatccgttac tgggtgatct
     6541 ccttccatga aaatacgcgg ttattgttaa acagcaggct gattactccg taaaggagaa
     6601 aaatattcat cttgcagcct gattatcagg tgtgtttatg tttactgagg tgagtatttt
     6661 tcagtatatt gtcggggcag tcagtgggag atattaaatt tacgtttaga gttatcttgc
     6721 ttctgctgtg tatttttata agagtgcgca cgtaatgata tctgttgata ccatgttgtt
     6781 aagtgtttcc cttccgggtc atgcgtactt gcaaaatggc atctgaatcg atcacggttt
     6841 ggccaaaccg tgatcgaata gagaggaata cgcacgtaat gattaaatgc ttttgttaca
     6901 tcattcttcc ggcgtgaata tttatccagt tccctttttt atttgtgtag tgatactgtc
     6961 ggaatagcgt tcagtaatgt aaagtgtcct gttaaaaatc tttaataaat gtgtcaccct
     7021 ggtcaggaaa tatctgctga gatattatta gttggtggtg tttttactga gtaatagctg
     7081 gctttttatt cctgaactga aatactgtcg ctcgtcaggg tttaattaaa actggatgat
     7141 gatgtgaaaa tgaaggctgg tttttgtgtt tgtcgaggaa gtgttgtttt tatccattct
     7201 catcattggc tggctgcgat tatacagaag tatttgaaga aaaaatggtg cattttatct
     7261 gtgccggcag ggagggggat tttcctgata tgtcagtatt aaatggtttt tgttatttat
     7321 aaaaatgtca tcctgtccat agaatattgt actgagtact cagcattcag cgcaggaact
     7381 gtcatcttct ttattttatt ctccttcagg agggcagttt tctgaaagcg ttactccggg
     7441 gaaactgttt atgtttattc ccggcaggaa acccgtacct gcaataccct ggatgatgct
     7501 ttttgctggt acgttgttgt gtgaaaatta atcaacatgt gtgctccgga gagcgcccgt
     7561 agacgcacag attcattctg acattcttta gcgacaaaat accgagtggt tattatttaa
     7621 cctgaaaatc gacagaaatt tttatcctgt attgattctg ctgctatttt tgctattagt
     7681 agcaatagtt gcggtacttt atgttctgtg tgttgtgagg tcattatgcc atactttcag
     7741 tatcctgatg agtttcccct gagctcgctg cctccactca ttcgtgatgc cgttattgaa
     7801 gctcagcaaa tcacgcaggc tcccctgggg cttgtggcag catctgccct gggcgctgtt
     7861 tcacttgtct gccagaatct gattgatgtc tgcagactga atacactacg ggggccggtt
     7921 tctcttttct tactgacgct tgctgagtcc ggagaacgaa aaaccgcagt ggacaaatta
     7981 ttaatggagc ccctgtacca gcaggagatg ctgttatatt ccagacacaa aaatgaactg
     8041 acgtcatgga aaaataagga agagatttta aaggcgcaga aaaaagcatt gttgtcaaaa
     8101 ctgaataaag aactccgcaa aggcgcggat gagtcagaaa cgctcagaca actggaagcg
     8161 cttcagaaaa acagagagga aaaacctgta agatataaat tcatttttaa tgatgccacg
     8221 acagcagcaa ttaaagatca gctttgtggt cagtggcgtt cagtgggtat catgtctgat
     8281 gaagccggta taatatttga tgggtatacc ctgtctgagc tgccttttat taacaaaatg
     8341 tgggatggct ccgtattgtc ggtggacaga aagaatgaac ccgaacagat gattgaaaat
     8401 gccagaatga cattatccct catggtacaa cccggtcttt ttgaccgata tatggagcga
     8461 aaaggttctg ttgccaggga cagtggtttt cttgcccgtt gtctaatcag taaacctgcc
     8521 accacgcagg ggaaacgctt tattaacgga gctgtgaccc cgggaggatc attaacggca
     8581 tttcatgaac gattaatgga actggccaga ggcagtattg aaaaatccag taaggatgaa
     8641 cgttattgtc tgcatttttc tcctgaagcg caaaaaatat ttattgatca ctataatgtt
     8701 ctggagcagg atttgtctcc ttccggaccc ttaagccagt ttcgggggca tgtttcaaaa
     8761 aaaacagaaa acattgcgag aattgctgcg ttattgcagt atttcagtca gggtgaaggg
     8821 aaaatctctg ccgatatcat gacgtctgct gtcgtaataa gttcgtggta cacagatgaa
     8881 tataaaaaat tatttgcgtt acctgatgaa tctgaactgc agctgcagga cgcccgggag
     8941 ctgctcgact ggctcattga ggagtgccgc ggagaatgcc cgccccgtgt caggaaaaat
     9001 tatattctcc agtgtggccc gggccggttc aggaacagga aaaagctgaa cgtcctgctg
     9061 aacattcttg catctcaact caggctctct gtcgtgcagg aaggaaaaac cacctatgtc
     9121 ctgctttccg aaataaacaa tatcacgctg gataaactga atgcgtgcta cagtcagaaa
     9181 ctgataagat ggcatgagca atcctttggc ttataatgtt ccctgttgct gagacggatt
     9241 attcagtggt ggtatcgcag tatgcccgtc accccccgct tctgcatgag ggtgacggag
     9301 gaaaagtaag tgtgcggagg cccgggagcg ttatgaatca acaggcgtat aacaggtact
     9361 cgtcaatcat cagttccagc tcgcgctttt cccgtttgcg cgccacagcc cagttttcaa
     9421 gctctgcatg tgatttatgc tttaactctt cagtccaggg ctgcatggag tgggcacaca
     9481 tcagatcata ccagcacagc catatcagcg tgtggtagag atcacgatcg tcgaaacgcc
     9541 ggagcaggct gttcagcctg tttgccagcc ggttttcacc cagacgatgt agctcactga
     9601 gtaaaacgcc gcccagatta aaatgtgtaa gcagcatgat ttcatgggtc tgaatgctgg
     9661 tcattaatcc ttcctgaatg gcaggaatga acggtggtgt tcccgggttg acgacctgat
     9721 agagtccctg gggggccacc gaaatgatgg tgcctttttc cggcagaaaa aagtgtgcca
     9781 gggtcagacg gtgataacca tgaagatgcg gagaccccat tccatgagcg acgataagcc
     9841 tcgtcacgct tgtcatccat ttctgatgct ggatgagcag cagctcacac cggttttcaa
     9901 gtttttgcat aatattctct cctttagcgc gcagcaaaag gcctccgggg gccggatcat
     9961 gcctttgctg cggttgaacg gataacggtc aggaattacg gacgggactg tgcaatacgc
    10021 gcctgcagcc aggtttccac ttcacttttc agccagcggg aactgcggcc cagtttgatg
    10081 ggggcaggaa aggcaccatc cttgatgagc ttgtaaaacc atttatcggt caggccggtc
    10141 agttgagtga taaatgccat gtcgaccatc tggtcatcca tcagtgaaac tggggtattc
    10201 atgaaaatgt tcctccggat gttgttaatc tcaaccggcg ttggtgatga aaacaacgca
    10261 ccaccttcac cggcataaca accggggaga aacgtaaaaa ataatcggga atgtgaccgt
    10321 caggagataa acggggggat tgcggtggct gagccgtacc ggtatgtcgg gtgattgttg
    10381 cgaaacgatg cttccgcacg cgcacgggag gactttcgtg agacaagggg aaaactggcg
    10441 ttctgataat tgcagagttg tttttgaagg cggttgtggt gcatcagcct gtgacctgtg
    10501 ggcaggcgac aggtatcact ggtgggataa gactgtctga ttggtattat aaatatataa
    10561 gagagctcaa acacatgagt tcatatatgt gaaataaatc tgtgagtgtg atcagcagac
    10621 cagtctaccc agtagcaaca gattgcgttg tactcacagg gttatccctg ataactggcg
    10681 tataacctgc gtacataaaa cgtacctgca atgcacctga acagagtgca ttaagcatcc
    10741 gcctgagcgg taaacgtatc cgatctggtg agtgacagtc tgagagcatt ctcagtaatc
    10801 gcccccttgt atcctggaaa ttggttaaga gcgattagat ttggcaaggt gtatgcagcc
    10861 agcagcttaa cacctcagct aactggctgg ttaccttcag gttgtcgcaa tggcatcgga
    10921 tatccggtgt cggataacta aacgtagcgt taaggcgata ttgctgagag accggttaag
    10981 tggtggcacg ggagtaatga gtttggggag ggtaccagaa tgccagtcag gtgcggtatg
    11041 ctggcagtat cacgttgatt gcagcataaa gtaacgagag acgaatggaa gttaaaacca
    11101 gagcagttcc gtcgttgttc atgccgggag acgctggaaa aagtgagttc ccacacacgt
    11161 tataaactta cccctgcgga gctggaagcc tttaactctg cggtcgatca ccggctggca
    11221 gaactgacaa tgaacaaact ttacgatcgt gtgccggctt ccgtctggaa atatgtcacc
    11281 tgaatcttga atccgcgcta cgatgactcc ggtgttatct gtgcaccgga gaaccgggaa
    11341 cattaagcgg tatagcgaca acaaagaatt cgaaatcaaa agggaatggt gtacagaggg
    11401 gcaggcgttc aggcattacc ggcctgtatc ttctgaaggg gactcgtatt tgtcggggct
    11461 gagtattccg ctcgattcga tcacggattg gcagaatacc tgactgtgat tgattcgggt
    11521 accattttgc agggatccgg tgcgttactg tctggcacaa caaacgcagc gggtcgctct
    11581 gccccgccga ttcctgataa tccctcccca gtaacctact gcaacagtac aaatacactg
    11641 acgcctgaaa cggtaaccag aatcagcacc agcagtaacg tcagtaaaca gagcagacga
    11701 tcggtcttcc tgtaataacc gaaggggtcg acagcgccac aatacgggca acatactgta
    11761 ttatgagtaa ttgcgttacc acagtccctg cacttctttt tgcgtatcac gatagctccc
    11821 tcgccacgcc ttatccgtaa ccggtttttt acattaaaaa aaacaacgcc gggaattcaa
    11881 tcgtcagtcc ggagacgacc gttcaggtta tcacagagag cctgagcagg gactctgcgt
    11941 attttttacg acgtggctat tccgtatgaa ccatacggag atttaaccat gacctacaaa
    12001 tacaacccct tctggcagca acgtattcgt gagacggtgc ggcacgcact gaatgttcat
    12061 ccccgcctga cggcattgcg ggttgacctg cgtttcccgg atgtaccggc agcaacggac
    12121 gcagctgtga tatcccgctt catcaatgcc ctgaaagccc gaatcgacgc ttaccagaaa
    12181 cgtaagcatc gggaaggtaa acgcgtgcat cccacaaccc tgcattacgt ctgggcccgg
    12241 gagtttgggg agcttaaagg caaaaaacac tatcacctgc tgctgctggt caaccgggat
    12301 acctggtgtc gtgccggcga ttaccgcgct ccggggtcac tggccgggat gattaaacag
    12361 gcctggtgca gtgccctggg agtggatgcc ggacgttatg acacgctggc acattttcct
    12421 gacaagccag cggtgtggct ggaacgcgat gatgaaaccg gctttcagca ggtgctggaa
    12481 cgtgctggct atctggcgaa ggagcatacc aaagctcgcg gcaccggtga gcgcaacttt
    12541 ggctgcagtc gtggctgagc cagacagacg agttatctga cttcagtgtg atgacgctct
    12601 cacggccacc acactgattt tcccttctga gactgaactg aaaccgccat tcacacctga
    12661 tcacgctgcc cgtaccggga cggctgttgc ctgcgtgtcg tctgacggta aggaacatat
    12721 actatgtacg caaaatcctt tctcgctctt gatggcaacg gacgtctgac gggggcccgt
    12781 accgcacaga ctgcgcctta cgaccgctac acctgccacc tttgcggcag tgcactcaga
    12841 taccacccgc aatacgacac tgaacggccc tggtttgaac acgctgacga agggctgacg
    12901 gagcacggtc atgagtgtcc ttatgtcagg ccggaacgca gagaaatacg gttgattaaa
    12961 cgtctgcagc aattcgtccc ggatgcctta cccgtggtgc gtaaagccag ctggcactgc
    13021 agacaatgtc accacgatta ttatggggag cggtactgca cacactgcca gaccggacgc
    13081 ttcagtgagg agggggtagc agaatgaacg gtataaaaac ggggctgggg atcacgccgg
    13141 gtgaacacat tatcagtgcc gactctgcgc tcagcagaaa tatccggcat tg
//
LOCUS       DAPHCV010000121        13052 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.122, whole genome shotgun sequence.
ACCESSION   DAPHCV010000121 DAPHCV010000000
VERSION     DAPHCV010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.122"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(37..402)
                     /locus_tag="RH033_003827"
     CDS             complement(37..402)
                     /locus_tag="RH033_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDV2992949.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            complement(693..1679)
                     /gene="yqjG"
                     /locus_tag="RH033_003828"
     CDS             complement(693..1679)
                     /gene="yqjG"
                     /locus_tag="RH033_003828"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HDV2992950.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFNHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            complement(1749..2141)
                     /locus_tag="RH033_003829"
     CDS             complement(1749..2141)
                     /locus_tag="RH033_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDV2992951.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(2328..2627)
                     /locus_tag="RH033_003830"
     CDS             complement(2328..2627)
                     /locus_tag="RH033_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HDV2992952.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRS"
     gene            complement(2617..3021)
                     /locus_tag="RH033_003831"
     CDS             complement(2617..3021)
                     /locus_tag="RH033_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDV2992953.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(3024..3329)
                     /locus_tag="RH033_003832"
     CDS             complement(3024..3329)
                     /locus_tag="RH033_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HDV2992954.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(3367..3735)
                     /locus_tag="RH033_003833"
     CDS             complement(3367..3735)
                     /locus_tag="RH033_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HDV2992955.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(3882..4265)
                     /gene="mzrA"
                     /locus_tag="RH033_003834"
     CDS             complement(3882..4265)
                     /gene="mzrA"
                     /locus_tag="RH033_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HDV2992956.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(4269..4931)
                     /locus_tag="RH033_003835"
     CDS             complement(4269..4931)
                     /locus_tag="RH033_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV2992957.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(5276..6052)
                     /gene="exuR"
                     /locus_tag="RH033_003836"
     CDS             complement(5276..6052)
                     /gene="exuR"
                     /locus_tag="RH033_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HDV2992958.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQATDNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDARTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            complement(6182..7480)
                     /gene="exuT"
                     /locus_tag="RH033_003837"
     CDS             complement(6182..7480)
                     /gene="exuT"
                     /locus_tag="RH033_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HDV2992959.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVDTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQESHNDPAPQH"
     gene            7963..9375
                     /gene="uxaC"
                     /locus_tag="RH033_003838"
     CDS             7963..9375
                     /gene="uxaC"
                     /locus_tag="RH033_003838"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDV2992960.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            9390..10877
                     /gene="uxaA"
                     /locus_tag="RH033_003839"
     CDS             9390..10877
                     /gene="uxaA"
                     /locus_tag="RH033_003839"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDV2992961.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQELPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIAHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALASAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            10960..11514
                     /locus_tag="RH033_003840"
     CDS             10960..11514
                     /locus_tag="RH033_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HDV2992962.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTTPSAVDERG"
     gene            complement(11519..12763)
                     /gene="sstT"
                     /locus_tag="RH033_003841"
     CDS             complement(11519..12763)
                     /gene="sstT"
                     /locus_tag="RH033_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDV2992963.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVIF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
BASE COUNT         3309 a         3378 c         3340 g         3025 t
ORIGIN      
        1 gccggatgag tcagacatcc ggcgagtaga tttgttttaa ggttccagtt ttggatcagg
       61 tccaaagcgg ttttcgcctg gcgtacccgc ctggcagtta aagacgatga taataagcca
      121 gccgataaac gggattaaga acagcagtgc ccaccacgcc gagcggtcgg tgtcgtgcag
      181 gcggcggaac tgaactgccc accatggtaa aaacaccaga ataccgtaga ttgtcgtcag
      241 gatgccttcg ccaccggcac gttgccagcc taacatttta tccagcagcc ccagcacgaa
      301 cgtaaagatg atgttgacca gaataaacat ccagtactct ttgcgccgcg cacgaccccg
      361 gaaaccgaca taatttttta gtactttcag ataccagtcc atttttgcct caattttgct
      421 caatcacttg tttttaaagc agcaaattaa gaatagacgt gaatgaaatg gcagtaaata
      481 ttcgttcaag gaattaataa agtgccgtaa agcgcgctaa gactgatgac aaacgcaaaa
      541 tcgccagatg cgctacgctt atctggccta cgcggcattg taacatattg aatataaacg
      601 ttttgtaggt tggagaagac gttcacgccg catccgacat aaataacacg cacttagcta
      661 ataatcagag gcacctcgta aggtgccttt ttttaaccga agcgaacatc tcgtccatgc
      721 ggttcatcga gatcctgcca cggaccaatt gaaataatcc ccgtagggtt gatggtctta
      781 tggctgcgga agtaatgatt acggatgtga ttgaaattga cagtttcggc gatccccggc
      841 atctggtaga tatcgcgcag aaaaccgtac agattcaggt aatcgctgat gcggtgctta
      901 tcacacttga agtgggtcac atacaccgga tcaaaacgga ccagcgtggt ccacaggcga
      961 atatcggctt cggttagctg attgccggtc aggtaacgat gctggcctaa aatctgctcc
     1021 agtcgcgcca gcgattcaaa cactttcgcc accgcctcgt cgtaagcttg ctggctggtg
     1081 gcaaaaccag ctttatacac gccgttgtta acggtgtcat aaatccagcc gttaagttcg
     1141 tcaattttcg tttgcagggc tggtgggtag taatcacccg ctttcgcgcc cagcgcatca
     1201 aacgcggtat tgaacatgcg gatgatttcc gctgattcgt tgctgacgat ggtgtggttc
     1261 tttttgtccc acagtacggg aacagtaact cgtccgctgt agtgtggatc ggcgtggaga
     1321 taaagctgat acaaaaattc atgttgatag agtgtatcgc cggttgcacc cggaaaactg
     1381 tcatcaaagg tccagccgtt ttccagcatc agcgggttca ctacagaaac ggaaataaac
     1441 ggttccagtc ctttgagttt gcgcatgatc agcgtgcggt gcgcccacgg gcaggcgagt
     1501 gaaacataga gatgataacg atctttctct gcgataaaac cgcctgtgcc agtggggcct
     1561 ggcgcgccat cggcagtgag ccagttacga aatgcggaag ctgaacgttg aaatttaccg
     1621 ccggtagatt tggtatcgta ccaggtgtca tgccagacgc cgtcaatcag ttgacccata
     1681 tttctctcct ccaatagcaa aaacgaggag cttcctcctc gttttttatc gttaagtata
     1741 gcgcgtgctt accacttttt attcagcagg cggtcgatgc tatacgcgcc cggaccggta
     1801 attgccagca gcaggaatcc gccagaaatt gtcaggtttt tcatgaacat cagcgagttg
     1861 acgccttcag caaagttgct gtgaaataaa aatgccgtca gcagcgtaaa gcccgcagta
     1921 aacagggctg tggtgcgagt caggaaaccg aacaggattg ccagaccacc accaaactca
     1981 agcagaatca ccagcggcag cataaaaccc gggacgccca tcgcttccat atattgttgg
     2041 gtacccgcgt aaccagtaat ttttccccag cctgcggtaa taaacagaat cggcattaaa
     2101 atgcgcgcta ccagtacacc aacatcttct aattttttca tcatactctc cactgcgccg
     2161 ctcaggctgc tgattgtttt tttgtgcaag ggcgcgctat tagcgtcgtt gctgtcgatg
     2221 gagagaatca taaacgtggc gaagcgtgat tgttagcaag gaaaactgtc aaatattttc
     2281 aaaaaatttg agggataagg ccggaatttc tccggccaga gaggaagtta actgcgaagc
     2341 tgttgttgct tgagggttgt tttcaccaga cgccaggcgc tccatacacc aaaaccgcgt
     2401 ctggcccagc ggaccagcat attaggatgg cgaatcgtcc agatagccat cacgctactg
     2461 ccaaccagcg cccaggagcg cagactcaac agcatattcc agcgacgatc gtaagcgcct
     2521 gttgcctcca gccattcacg acgactggcg gaaagatcca gccgttgttg ctggatctgg
     2581 ctaagcagtt gcgccttacg tcgttcacgt tcgactttac tgctcacggg actcctcctc
     2641 gagcagctgc cgatcgtttg ccagctcatg gcgcgtgtgg cgcagcaacg tagacttacg
     2701 cgatttacgt aacgtccaga tgccgccaat cagtgccagt agcagcaaca ccacggtggt
     2761 ggcaatcatc gcattcaggc gatattgcgg atcaaccgcc cagataatta gcaccatcag
     2821 gctcataaga ccaaatgcag cgaaaagcat cgtcaggccc agcatcagta aaagttgaaa
     2881 gagattcgct ttttcctctt ccagctccac caccgccagc cgcagacgtg tctccaccat
     2941 ttcaaccatg atggaaacaa ttcgctgccc gatgcccaga acgcttttac cgggcccttg
     3001 cgcgtgatga gtgtccgcca taattaacga cgcgacagca gaacgccgag cactacaccg
     3061 attgctgcgc caatgcccac gcccgtccac ggattttcac gcacatactc atcggcacgc
     3121 gccgccgcga cacgggtttg tttggcaatg gcatcaccgg tttcacccag gcgataacgg
     3181 ctctgtttca gtgcctgctc cgctttgcta cgaatcttac tcaactcttc tttcgacttc
     3241 tcgccagatg agctaagcac ctcttccagc gtatcggaaa gggatttcaa ctcagcacgc
     3301 agatgttccg tagtgtgttc tttcgacata gttttctcca ggtgagtaag tagtgactat
     3361 tgtgagttag tagtcgcgcg cttccagctt tttcagctct tcctgcgctt ctgccagttt
     3421 ccgttcgcgt ttggcaatct tatctgcatc gcctttttgc ttcgcctcgg ctaaatcttg
     3481 ctggcgttcc gccacctcat ctttctgctt tgcgattttc ttctgatgat cggcacgcag
     3541 ttggctatct gaacagttgg cccggacttc actcagggct ttattcagac cgtcaatacg
     3601 attctggttt tggtgttttt cggcatagct gatctcctta aggatatttt gctccttttc
     3661 ctgacacagg gtagtggcat aactaccggc actaagagca aagagagaaa cagctaaagc
     3721 gatgcggtat ttcattcttg aaccttccat tgtgattcgg acctattaca aaggaacgat
     3781 tgtccagaca acgaagcggt agtgttaacc accgcctcat taatcataga cagttacggt
     3841 gaaatcacca aagtgagtga tctgtttcgg ataacaggaa gttatccgaa gcgatgagag
     3901 ttatcccgca accgggtcag ccactgcata gcctgactgt tattgtcctg ctgcgcaatg
     3961 atgtagccat ggggcaatgt tctgtcgagg accgcttttg cggcggcgct ctggtcgctg
     4021 gagtcaaagg taattaacag agtgtcgttt ttgggggtga tacttttgaa aggaatgccg
     4081 tgagcgtcaa ggtgatgcca gattgaaaaa ccgtctggca tcgttgtccc ttgatgaacg
     4141 gcacgaatcg ccagcgtaga ctcttgctgg cgaaccgcgg accaggccag caacagtgtg
     4201 ccgaccagaa gtaaaacagc gccggaccag gctagctggc gaagcgacat gcgaggtatt
     4261 tgcatccctt acccccgatt tccatatttc tttttccata acacgaccag agaacctgcc
     4321 aggccaaaca ccagcagcac caccgggagc agcatcaggc atgacatcag ctggtcctcg
     4381 tactttaaaa ataccggcgt tttgccgagc atgtaaccca gagttgtcag gatcaatacc
     4441 cacaacaaac cactcatcca gttgaagaac tgaaagcgcg cgttattcag ccctgataac
     4501 ccggcaatcg tcggcagcag tgttctgaca aacgcaataa agcgaccaat taacagcgcc
     4561 gataaaccgt gtttatgaaa aagatggtgt gcgcgttgat gataatgcgc gggtaaatga
     4621 gatagccagt tttgtacggt gcgggtattg cccagccatc gcccctgaat atagctgacc
     4681 cagcagccga ggctggcggc aacggtcagc agcagaatcg tttgcggata gcccatcgcg
     4741 cctttcgcaa tcaatacacc gaccaacacc agtaagctgt cgcccggtaa aaaggctgcc
     4801 ggaagcaagc cgttttcaag gaacaaaatg acaaacaaga caaaatacaa catgccaatc
     4861 atcgacggat tggccagggt ttcaaaatcc tgcgcccaca gggcttgcag caattgggtc
     4921 aaaagttcca ttcattattc ctggtgaatc ggttaacgcc acgctggttt taaatgatgc
     4981 actaccgcaa tgttattgct gtcttggtat gttctgcggg tttgatacat caacacagcc
     5041 tgaactattc cctgttaaat cgtgagataa gaaagcattc aaaagcataa attcaaattg
     5101 aatctaattg taacaaaggt caacgttgtt cgttatcaaa attacatgtc cgtggagtct
     5161 gattttgctg aatgcagggg agggaggcga gaggatttac acaggctgac actttatact
     5221 tttcgcttac cgacgagaca aacaggcgca ctgtagcgcc tgtcggacag gaatttcatt
     5281 tactgccgct ggtggctgta tcgagatgca ccaccgggtt ttcggcgaac agatagcggt
     5341 cggcattgaa ctcgaaatcg tcgctggttt cgttaaataa catgatcttg gtgttttcca
     5401 ggtgctgcca cattgccagc ttagcagcat gaggatcttt gcgaatcagc gccttgagga
     5461 tttgatcgtg gtcatcgcac cagttatcga cggtacgggc atcaatgtgt tcgtgcagtt
     5521 ttttccagta cgggttatga ctacgctggg tccacatttt ttccacgata gccgccaggg
     5581 cggagttctg cgtcgccagg gctacctgaa tgtggaactg caaatcccac tcggaatcac
     5641 ggaagcattg ttcgccgcgc gcctgctcct ggatggccat cagtttcatg atgtcctgtt
     5701 tcgtcacctg agttgccgcg aactcggcaa tattactttc gatgagctgg cgagcctgga
     5761 gcaactcaaa cggaccgtaa ttggcgaatt ccatattatt gtcagtcgcc tgctgatggc
     5821 gcggctggtt ggaaaccaca tgaatacctg aacctttacg cacttcgaca tagccttcaa
     5881 cttccagcat gatgatggct tcacgaacca cggtgcggct gacgttcttt tcatcggcaa
     5941 taaagcgttc agcaggcagt ttatcaccca ccagatagac gccctgttcg atgcgctctt
     6001 tcagatcagc ggcaagttgt tgatacaaac ggcgtggttc agtgatttcc atatgcgctc
     6061 caggcatgat acggcagact atatttgtta taccactttt gcgagtttag ctctactcac
     6121 agtgatgaaa aagccgcctg agaaggcggc tttgaaggga aactaaaact tacacgaagc
     6181 cttaatgttg cggtgcggga tcgttatgtg attcctgcgc cacctcaatc gccggtttgt
     6241 tctgcaacac ggtccagata accagcgcac ctaacaggtc gaacactgcc agaactgcga
     6301 acagcgggct gaagccgatg gtgtcagcca gtgcaccgac taccagtgca aacagcgtac
     6361 ttgccagcca tgcggacatc ccggttaaac cgttcgccgt cgccacttcg ttacgaccga
     6421 acacgtcaga cgagagcgta atcagtgcgc cagacagtgc ctggtgagca aaaccaccga
     6481 tacacagcag cataatcgcg acatacgggt tggtgaacag accgatcata cccgggccaa
     6541 tcatcagcac cgcacccagc gttacgacca tcttacggga aacgatcagg ttcacaccaa
     6601 accaacgctg gaacagtggt ggcaggtaac caccgaggat acaaccgagg tcagcaaaca
     6661 gcatcggcat ccaggcaaac atcgcgatct ctttcaggtt aaagccgtaa actttaaaca
     6721 tgaacagcgg gatccacgcg ttaaaagtac cccatgccgg ttctgccagg aagcgcggca
     6781 gcgcgatacc ccagaactga cggttacgca ggatctgacc aacggacatt ttcttcgctg
     6841 tgtcaacctg gtgctgggct tcctgaccat taataatgta gtcgcgttct tcatcggtca
     6901 gatgtttctg gtcgcgcgga tgtttataga aaatcagcca tgccatcgcc cagataaagc
     6961 tcaacgcacc ggagatgata aatgccatct gccagctgtg catcacgatt gcccatacca
     7021 ccagcggcgg cgcaatcatc gcaccaatcg aagaacctac gttaaagtaa cctaccgcga
     7081 tggaacgctc tttcgccggg aaccattcgg agctggcttt cagacccgcc ggaatcatcg
     7141 cggcttccgc ggcaccgacc gcaccacgag caacagccag gccgccccag ctacctgcca
     7201 gcgcggttgc accacagaac acggcccaca gcacagcaaa cattgcataa ccgattttcg
     7261 tacccagcac atccagtaca tagcccgcta ccggttgcat gaccgtataa gcagcagaat
     7321 aggctgcgat gatataggaa tactgttggg tggagatgtt taactcttcc atcagagttg
     7381 gcgcagctgc cgccacagtg ttacgcgtca ggtaaccaag cacggtgccg agcgtcacca
     7441 gtgcgatcat ataccaacgt aaccctttaa ttttacgcat gtaaaacctc atcgttttgt
     7501 tatatccgca ggaagcgcgg ccatcatccc gccagaggct gaagatgtta gttacgaccg
     7561 ggtaaccgaa aaaaaaccgg cacgccccga acgttgccat gaaagtttcg tgctaattcg
     7621 gcttccgtac cggttaaccc gattcatcta acaaatgaat gaatcggcaa tttgcattcc
     7681 gtcgacttat gatttgcgac ggcagaaaga taacttgtca tacaacttta aaaggtgaga
     7741 gccatcacaa atgtgcgaat atttgtaggg acattacctg acgacggcga ggccagtact
     7801 ggcgcggcct gcagcgagat ttaccacttt gagagtaatt tttttaacta cgtttattga
     7861 tctaactcac gaaaatatct tcggactctg gaaattggtg tgataacttt gtcagcatcg
     7921 caccataagc aagctagctc actcgttgag aggaagacga aaatgactcc gtttatgact
     7981 gaagatttcc tgttagatac cgaatttgcc cgccgtctgt atcacgacta cgcaaaagac
     8041 cagccgattt tcgattacca ttgccatttg ccgccgcagc agattgcgga agactatcgt
     8101 tttaaaaacc tgtatgacat ctggttgaag ggcgatcact acaaatggcg cgctatgcgt
     8161 accaacggtg tggccgagcg tctgtgcacc ggtgatgcgt ctgaccgtga aaaatttgac
     8221 gcctgggcgg cgactgttcc gcacactatc ggcaacccgt tataccactg gacgcatctt
     8281 gaactgcgtc gtccattcgg tattaccggt aaactgctgt ctccgtctac cgcggatgaa
     8341 atctggaacg aatgtaacga actgttggcg caggataact tctccgcgcg cggcatcatg
     8401 cagcagatga acgtgaaaat ggtcggcacc actgatgacc cgatcgattc tctggagcat
     8461 cacgcagaga tcgccaaaga cggctctttc accattaaag tgctgccgag ctggcgtccg
     8521 gacaaagcct ttaacatcga acaggcgacc tttaacgact acatggcgaa gctgggtgaa
     8581 gtctctgata ccgacatccg ccgctttgct gatctgcaaa ctgccctgac caaacgtctg
     8641 gatcacttcg ccgctcacgg ctgtaaagtg tctgaccacg cgctggatgt ggtgatgttt
     8701 gctgaagcga acgaagcgga actggacagc attctggcgc gtcgtctggc tggcgaaacc
     8761 ctgagcgagc acgaagtggc acagttcaaa actgcggtgc tggtgttcct tggtgccgaa
     8821 tacgcacgtc gcggctgggt acagcagtac cacattggcg cactgcgtaa taacaacctg
     8881 cgtcagttca aactgctggg gccggatgta ggctttgact ccatcaacga ccgtccgatg
     8941 gcggaagagt tgtctaagct gctgagcaag cagaacgaag aaaacctgct gccgaaaacc
     9001 attctctact gcctgaaccc gcgcgataac gaagtgctgg gcaccatgat cggtaacttc
     9061 cagggcgaag gtatgccggg caaaatgcag ttcggttccg gatggtggtt taacgatcag
     9121 aaagacggta tggaacgtca gatgacccaa ctggcgcagc tcggtctgtt gagccgcttt
     9181 gtcggtatgc tgactgacag ccgtagcttc ctgtcataca cccgtcacga atacttccgc
     9241 cgcattctgt gccagatgat cggtcgctgg gtggaagcgg gcgaagcacc ggcggacatc
     9301 aacctgctgg gcgagatggt gaaaaatatt tgctttaaca atgcgcgtga ctacttcgcc
     9361 attgaactga actaaggtct ggggttgata tgcaatacat caagatccat gcgctggata
     9421 acgtcgcggt tgctttagcg gatttggctg aaggcacaga agtcagtgtc gataaccaga
     9481 ctgttacgct gcgccaggat gttgctcgtg gacataaatt tgcgttaacg gatatcgcaa
     9541 aaggggccaa cgtcattaaa tatggcctgc cgattggtta tgcattggcg gatattgcgg
     9601 cgggggaaca cgtccacgcc cacaatacgc gcacgaatct gagcgatctg gatcagtatc
     9661 gctatcaacc tgattttcag gagctgcctg cgcaagcggc agatcgtgaa gtgcagatct
     9721 atcgtcgcgc taacggcgat gtcggggtgc gtaatgagct gtggatcctg ccaaccgtgg
     9781 gctgtgtcaa cggcatcgcg cggcagatcc aaaatcgttt cctgaaagag accaacaacg
     9841 ccgaaggtac tgacggcgtg ttcctcttca gccacaccta cggctgctca cagctgggcg
     9901 acgatcacat caatacccgc accatgctgc aaaacatggt gcgccacccg aacgcaggcg
     9961 cagtgctggt gattggtctg ggctgtgaaa acaaccaggt tgccgcattc cgtgaaacgc
    10021 tgggcgatat cgatcctgaa cgcgttcatt tcatgatctg ccaacagcag gatgatgaga
    10081 tcgaggccgg gatcgcgcat ttgcatcagc tgtataacgt gatgcgcaat gacaagcgtg
    10141 agccgggcaa actcagcgag ctgaagtttg gtctggagtg cggcggttcc gacggtcttt
    10201 ctggtattac tgctaaccca atgctggggc gtttctctga ttacgtcatc gctaacggcg
    10261 gcactaccgt gctgaccgaa gtaccggaaa tgttcggcgc agagcagttg ctgatggacc
    10321 attgccgcga cgaagcaacg tttgaaaaac tggtcaccat ggtcaatgac ttcaagcagt
    10381 actttattgc ccatgaccag ccgatctacg aaaacccatc accggggaac aaagcaggcg
    10441 gtatcaccac gctggaagat aaatcgcttg gctgtaccca gaaagcgggt tccagtgtcg
    10501 tggttgacgt gctgcgttac ggcgagcgtc tgaaaacgcc ggggctgaac ttgttaagtg
    10561 cgccgggtaa cgatgccgta gcgaccagcg ccctggcgag tgcgggctgc catatggtgc
    10621 tgttcagtac tggtcgtggc acgccgtatg gtggatttgt gccgacggtg aaaatcgcca
    10681 ccaacagtga actggcggcg aagaaaaaac actggatcga ctttgacgcg ggtcagctga
    10741 tccacggtaa agcgatgccg cagttgctgg aagaatttat cgacaccatc gttgagtttg
    10801 ccaacggtaa gcaaacctgc aacgagcgta acgacttccg cgaactggcg atcttcaaaa
    10861 gcggcgtaac gctataaagt cgaaatgagt tgttaataaa aaacggcgtt tcataatgtg
    10921 aagcgccgtt tgcttttcca tacctgctaa ggatttatca tgaccgcgta ttggctggcc
    10981 cagggcgtgg gtgtcatcgc ctttctgatt ggtatcacaa catttttcaa tcgtgacgaa
    11041 cgtcgcttca aaaagcagct ttcggtctat agcgccgtta ttggcgtaca tttttttctt
    11101 ctgggcacct atcccgccgg tgccagtgcc atccttaatg ccattcgtac attgattacc
    11161 ttacgcacgc gcagcttatg ggtaatggcg atttttattg tgctgactgg cggaattggt
    11221 ctcgcgaagt tccatcatcc tgtcgaacta ttgccggtta tcggcacgat tgtcagtacc
    11281 tgggcgctgt tccgctgtaa agggctgacc atgcgctgcg tgatgtggtt ttcaacgtgt
    11341 tgctgggtga ttcacaactt ctgggcgggg tcgataggcg gcacgatgat tgaggggagt
    11401 tttctgctca tgaatggcct gaatatcatt cgtttctggc ggatgcagaa aaggggaatt
    11461 gatccgttta aagtagagaa aacaacccct tccgccgtag acgaaagggg ttaaacaatt
    11521 aattacgcag ggcgctattt gccagacgat cgtcttctgc ctggcaagct gccgcagtga
    11581 acagcacgtc agttgacgag ttcagcgcgg tttcgcaaga gtcctgcaat acgccgatga
    11641 taaagccgac ggcaaccacc tgcatggcga tatcgttcga aataccgaac atattacagg
    11701 ccagtgggat cagcagcaga gacccccccg ccacgccgga tgcgccacag gcacacagag
    11761 aagccactac gctcaacagc agcgctgtgg gcagatccac cggaataccc agcgtattaa
    11821 ccgcagccag cgtcaacacg gtaatggtga ttgctgcgcc cgccatattg atggtggctc
    11881 ccagcggaat agaaacggaa taggtatcgc gatccagatt cagcttttca cacagcgcca
    11941 tattcaccgg aatgttcgct gcagagctgc gggtgaagaa ggcatacaca ccgctttcac
    12001 gcaggcacag cagcaccagc gggaacgggt tacgacgaat tttccaccac accagcaatg
    12061 ggttaaccac cagcgccacc agtaacatac agccaaccag cacgaccagc agttgcgcgt
    12121 agccccacag tgtggagaaa ccggtggttg ccagggtaga agaaaccaga ccaaaaatac
    12181 cgatcggtgc gaagcgaatc accagtttca ccataaaggt gacggcattc gacatatcgt
    12241 tgaccaggtt tttggtggtc tcgttaccgt gacgcagtgc gaaaccgagg ccaattgccc
    12301 acaccaggat cccgatatag ttacctttca gcagcgcgtc aatggggttg gaaaccatgc
    12361 tcattaccag cccgcgcatc acttctacaa tgcctgacgg cggcgaaata tcacccgcgc
    12421 tactggacaa gtgcagggta gaagggaagg caaaactgaa gattactgcg gccagagcag
    12481 cagagaaggt gcccagcaga tagaggaaca aaatagggcg gatattggtt ttctgcccgt
    12541 gctggtggtt agcaatagat gccattacca gcatcaacac caggatgggg gcaacggctt
    12601 tcagtgcgcc gacgaacaaa gtacctaaca gaccaacagc ttccgccgcg ggttttgaga
    12661 tccatgccag aagaatcccc agaacaaggc cgaccaggat ttgttttacc aggctgccat
    12721 gagccagacg ccggaatagc cccggtgaac gttgcgtagt catttttcga tcctttcatt
    12781 gtgttgtcgc acatccctgg tgcgttctgt cgacgcatct ttcaggatgt aacaaagtgt
    12841 ttgcaaggtc gagtataagg aaagttacag cttcgggaag cgaaaaatgc tggattttaa
    12901 agagaggtca tattttgcaa cttaatagtt acatttattt gacataaaca gagggaagga
    12961 ctggctacta aggctgatta gataacatat gggcaaaact atttggaata tgagaacggt
    13021 tgcgctcctt taatgtgcta tgccatataa ag
//
LOCUS       DAPHCV010000122        12967 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.123, whole genome shotgun sequence.
ACCESSION   DAPHCV010000122 DAPHCV010000000
VERSION     DAPHCV010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.123"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            230..1288
                     /gene="citC"
                     /locus_tag="RH033_003842"
     CDS             230..1288
                     /gene="citC"
                     /locus_tag="RH033_003842"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HDV2992964.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DENLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFCHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1303..1599
                     /gene="citD"
                     /locus_tag="RH033_003843"
     CDS             1303..1599
                     /gene="citD"
                     /locus_tag="RH033_003843"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HDV2992965.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1596..2504
                     /gene="citE"
                     /locus_tag="RH033_003844"
     CDS             1596..2504
                     /gene="citE"
                     /locus_tag="RH033_003844"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HDV2992966.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2515..4047
                     /gene="citF"
                     /locus_tag="RH033_003845"
     CDS             2515..4047
                     /gene="citF"
                     /locus_tag="RH033_003845"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HDV2992967.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAISSALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4051..4602
                     /gene="citX"
                     /locus_tag="RH033_003846"
     CDS             4051..4602
                     /gene="citX"
                     /locus_tag="RH033_003846"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HDV2992968.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGDILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4577..5455
                     /gene="citG"
                     /locus_tag="RH033_003847"
     CDS             4577..5455
                     /gene="citG"
                     /locus_tag="RH033_003847"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HDV2992969.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLNGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5506..6969
                     /gene="citT"
                     /locus_tag="RH033_003848"
     CDS             5506..6969
                     /gene="citT"
                     /locus_tag="RH033_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HDV2992970.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFNAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFANTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7083..7889
                     /gene="rna"
                     /locus_tag="RH033_003849"
     CDS             7083..7889
                     /gene="rna"
                     /locus_tag="RH033_003849"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HDV2992971.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8119..8529
                     /gene="rnk"
                     /locus_tag="RH033_003850"
     CDS             8119..8529
                     /gene="rnk"
                     /locus_tag="RH033_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HDV2992972.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8886..9314
                     /gene="uspG"
                     /locus_tag="RH033_003851"
     CDS             8886..9314
                     /gene="uspG"
                     /locus_tag="RH033_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HDV2992973.1"
                     /translation="MYKTIIMPVDVFEMELSDKAIRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAK
                     ELDADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(9435..11000)
                     /gene="ahpF"
                     /locus_tag="RH033_003852"
     CDS             complement(9435..11000)
                     /gene="ahpF"
                     /locus_tag="RH033_003852"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDV2992974.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDRAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(11171..11734)
                     /gene="ahpC"
                     /locus_tag="RH033_003853"
     CDS             complement(11171..11734)
                     /gene="ahpC"
                     /locus_tag="RH033_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HDV2992975.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            12106..12852
                     /gene="dsbG"
                     /locus_tag="RH033_003854"
     CDS             12106..12852
                     /gene="dsbG"
                     /locus_tag="RH033_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HDV2992976.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLSVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKMLNIIMGNK"
BASE COUNT         3092 a         3404 c         3265 g         3206 t
ORIGIN      
        1 taattaaaaa acttaaaacc attaaataaa taaaaaacca ctaactctat gtgaaataaa
       61 tcaaaatttc acgctgcaat actccttagg atgtatagcg aaaggagaaa agatatacct
      121 cgatcgcccc cctttctcct aagtgaaaat aaaaggttat cagtttgcaa catcgaacaa
      181 catttgttgc aaatcgataa caacatgcac cttcaggata ctattcatta tgttcggcaa
      241 tgatattttc acccgtgtaa aacgttcaga aaataaaaaa atggcggaaa tcgcccaatt
      301 cctgcatgaa aatgatttga gcgttgacac cacagtcgaa gtatttatta ccgtaacccg
      361 cgatgaaaat cttatcgcgt gcggtggaat tgccggaaat attattaaat gcgttgctat
      421 cagtgaatcc gttcgcggtg aaggactggc gctgacatta gccactgaat tgataaacct
      481 cgcctatgaa cggcacagca cgcatctgtt tatttatacc aaaaccgaat acgaggcgct
      541 gttccgccag tgcggttttt ccacgctgac cagcgtaccc ggcgtgatgg tgctgatgga
      601 aaacagcgcc acgcgactga aacgctatgc cgaatcgctg aaaaaatttt gtcatccagg
      661 gaacaagatt ggctgcattg tgatgaacgc caatcccttt acgaatggtc accgttatct
      721 gattcaacag gctgcggcac agtgcgactg gttgcatctg tttttagtca aagaagattc
      781 ttcacgcttc ccctatgaag accgactgga tctggtgtta aaaggcaccg ccgatattcc
      841 acgcctgact gtgcatcgcg gctccgaata catcatatcc cgcgctacgt tcccttgcta
      901 cttcattaaa gaacagagcg tcattaacca ttgttacacc gaaattgatc tgaagatttt
      961 ccgtcagtac ctcgctcccg cgctgggtgt aactcaccgc tttgtcggta ctgaaccctt
     1021 ttgtcgcgtt accgcccagt acaaccagga tatgcgctac tggctggaaa cgccgactat
     1081 ctccgcaccg cccatcgaac tggttgaaat tgagcggctg cgttaccagg agatgccgat
     1141 atccgcttcc cgggtacgtc aactgctggc gaaaaacgat ctcacggcta tcgcgccgct
     1201 ggtccctgca gtcacgctgc attatttgca gaacctgctt gagcactccc gccaggacgc
     1261 ggcagctcgt caaaagaccc ccgcatgaga aacaggtgaa aaatgaaaat aaaccagccc
     1321 gccgttgcag gcacccttga gtctggggat gtgatgatac gcatcgcccc actcgatacg
     1381 caggatatcg acctgcaaat caacagcagc gttgagaaac agtttggcga tgccattcgc
     1441 accaccattc tggacgttct cgcccgctac aacgtgcgcg gcgtacagct gaatgtcgat
     1501 gacaaaggcg cactggactg cattttacgt gcacgactgg aagccctgct ggcacgcgcc
     1561 agcggtatcc cggctctgcc atgggaggat tgccaatgat ttccgcttcg ctgcaacaac
     1621 gtaaaactcg cacccgccgc agcatgttgt ttgtgcctgg tgccaatgcc gcgatggtca
     1681 gcaactcatt catctacccg gctgatgccc tgatgtttga cctcgaagac tccgtggcat
     1741 tgcgtgaaaa agacacagcc cgccgcatgg tttaccacgc gctgcaacat ccgctgtatc
     1801 gcgatattga aaccattgtg cgtgtaaacg cgctggattc cgaatggggt gttaacgacc
     1861 tggaagccgt cgttcgcggt ggtgcggacg ttgtgcgtct gccgaaaacc gataccgctc
     1921 aggatgttct ggatatcgaa aaagagatcc tgcgcatcga aaaagcctgt ggtcgtgaac
     1981 ccggcagcac cggcctgctg gcagcgattg aatctccgct gggcattacc cgcgcggtgg
     2041 aaatcgctca cgcttccgag cgtttgatcg gtatcgccct cggtgcagaa gactatgtgc
     2101 gcaacctgcg tacagaacgc tccccggaag gaactgaact gctgttcgca cgctgttcca
     2161 ttttgcaggc cgcgcgctct gcgggtattc aggcgttcga taccgtctat tccgacgcta
     2221 ataacgaagc cggattcctg caagaagccg cccacatcaa acagctgggc tttgacggca
     2281 aatcgctgat caacccgcgt cagattgatc tgctgcacaa cctctacgca ccgactcaga
     2341 aagaagtgga tcacgcccgc cgcgtcgtag aagccgctga ggccgccgct cgcgaaggcc
     2401 tcggcgtggt ttccctgaac ggcaagatgg tggacggtcc ggttatcgat cgcgcccgtc
     2461 tggtgctctc ccgcgcagaa ctttccggca tccgcgaaga ataaggcaat caaaatgacg
     2521 cagaaaattg aacaatctca acgacaagaa cgggtagcgg cctggaatcg tcgcgctgaa
     2581 tgcgatcttg ccgctttcca gaactcgcca aagcaaacct accaggctga aaaagcgcgc
     2641 gatcgcaaac tgtgcgccaa cctggaagaa gcgattcgtc gttctggttt acaggacggc
     2701 atgacggttt ccttccatca cgctttccgt ggcggtgacc tgaccgtcaa tatggtgatg
     2761 gacgtcatcg cgaagatggg ctttaaaaac ctgactctgg cctccagctc cctgagtgat
     2821 tgccatgcgc cgctggtaga acacattcgc cagggcgtgg ttacccgcat ttatacctcc
     2881 ggcctgcgcg gtccgctggc ggaagagatc tcccgtggtc tgctggcgga accggtgcaa
     2941 atccactctc acggcggacg tgtgcatctg gtacagagcg gcgaactgaa tatcgacgtg
     3001 gctttcctcg gcgtcccgtc ctgtgatgaa ttcggtaatg ccaacggcta caccggtaaa
     3061 gcctgctgcg gctccctcgg ctatgcaatg gttgatgccg acaacgcaaa acaggttgtg
     3121 atgctcactg aagaactgct gccttatccg cataatccgg caagcattga gcaagatcag
     3181 gttgatctga tcgtcaaagt tgatcgcgtt ggcgatgctg caaaaatcgg cgctggtgct
     3241 acccgtatga ccactaaccc gcgcgaactg cttattgccc gtagcgctgc ggatgtgatt
     3301 gttaactctg gctacttcaa agaaggtttc tccatgcaga ccggcaccgg cggcgcttcg
     3361 ctggcggtaa cccgtttcct ggaagacaaa atgcgtagcc gcgatattcg cgccgacttc
     3421 gccctcggcg gtattaccgc aacgatggtt gatctgcacg aaaaaggtct gatccgcaaa
     3481 ctgctggatg tgcagagctt tgacagccat gctgcgcaat cgctggcccg taaccccaat
     3541 cacatcgaaa tcagcgccaa ccagtacgct aactgggggt cgaaaggcgc atcggttgat
     3601 cgtctcgacg tggtggtact gagtgcgctg gaaattgaca cccagttcaa cgttaacgtg
     3661 ctgaccggct ctgacggcgt actgcgtggt gcttccggtg gtcactgcga taccgcgatt
     3721 tcttctgcgc tttccatcat cgtcgcgccg ctggtacgcg gtcgtattcc gacgctggtg
     3781 gataacgtgc tgacctgcat caccccgggc tccagtgtcg atattctggt cacagaccac
     3841 ggtatcgcag ttaacccggc acgtccggag ctggcagaac gtctgcagga agcggggatc
     3901 aaagtggttt ctattgagtg gctgcgtgaa cgtgcgcgtc tgctgaccgg tgaaccacag
     3961 ccgattgaat tcaccgaccg cgtcgttgcc gttgtgcgtt accgcgatgg ctcggtgatc
     4021 gatgttgtgc atcaggtgaa ggaataagcc atgcacctgc ttcctgaact cgccagccac
     4081 catgcggtgt caattcccga gctgctcgtc agccgggatg aaaggcaagc acggcaacac
     4141 gcctggctca agcgccatcc tgttccactg gtctccttta ccgtggttgc gcctgggccg
     4201 attaaagaca gcgaggtcac acgccgaatt tttaatcatg gcgtgacagc cttgcgtgcc
     4261 ttagccgcaa aacagggctg gcaaattcag gagcaggctg cactggtttc cgccagcggg
     4321 ccggagggca tgttgagcat tgccgccccg gctcgcgacc tcaagctcgc caccattgag
     4381 cttgaacata gtcatcctct cgggcggtta tgggatatcg atgtcctgac gcccgaaggc
     4441 gacattctct cccgccgcga ttattcactg ccgcctcgcc gctgcctgtt gtgcgagcaa
     4501 agcgcagctg tttgcgcgcg tggaaaaacc catcaactga ccgatttact caaccgcatg
     4561 gaggcgctgc taaacgatgt cgatgcctgc aacgtcaact aaaaccacaa agcttgcgac
     4621 gtcattaatc gatgagtacg ccctgctggg ctggcgcgcc atgctgactg aagtcaacct
     4681 gtcaccgaaa ccaggcctcg tggatcgcat taactgcggt gcgcacaaag atatggcgct
     4741 ggaagatttc caccgcagcg cgctggcgat tcagggctgg ttaccccgtt tcattgaatt
     4801 tggtgcctgt agtgcagaaa tggcaccgga agcggtactc aacggattac gcccaattgg
     4861 tatggcttgc gaaggtgata tgttccgcgc cactgcgggc gtaaacacgc ataaaggaag
     4921 cattttttct ttagggctac tgtgtgcggc aattggtcgt ttgcttcagc tcaaccagcc
     4981 ggtaacgcca acaaccgttt gttctacggc ggcaagtttc tgccgtggcc tgaccgatcg
     5041 cgaactgcgt accaataatt cacgactgac ggcaggtcaa cggttgtacc agcagcttgg
     5101 ccttaccggc gcacgcggtg aagccgaagc gggttatcca ctggtgatca accacgcctt
     5161 gccgcattac ctcactctgc tggatcaggg gttagatcct gagctggcat tgctcgatac
     5221 cttgctccta ctgatggcta ccaacggcga taccaacgtt gcatcgcgcg gtggcgaggg
     5281 gggcctgcgc tggctacagc gcgaggcgca aacattattg caaaaagggg gcattcgaac
     5341 ccccgccgat ctcgattatc tccggcagtt cgacagggag tgtatcgaac gaaatctcag
     5401 tccaggcggc agtgccgacc tgctgatcct tacctggttt ttagcacaga tttaattatt
     5461 taagcacttg ataaatttgg aaatattaat tttcggagaa cccgtatgtc tttagcaaaa
     5521 gataatatat ggaaattatt ggcgcccctg gtggtgatgg gtgtcatgtt tcttatccct
     5581 gtacctgacg gtatgccgcc gcaggcgtgg cattactttg cagtgtttgt ggcaatgatt
     5641 gtcggcatga tcctcgagcc aattccggca acggcgatca gttttattgc ggttactatt
     5701 tgcgttattg gcagtaatta cctgctcttt aatgccaaag aattagctga cccagcgttt
     5761 aatgcgcaaa aacaggcgct gaaatggggt ctggctggtt tttccagcac cacggtatgg
     5821 ctggtatttg gcgcatttat ttttgcatta gggtatgaag tttccgggtt aggccgtcgt
     5881 attgcccttt tcctggtgaa attcatgggc aaacgcacgc tgacgttggg ttatgcgatt
     5941 gtcattatcg acattctgct ggcaccgttt acaccgtcca acaccgcgcg taccgggggt
     6001 acggtttttc cggtcattaa aaacctgccg ccgttgttta aatcattccc gaacgatccg
     6061 tccgcgcgtc gtattggcgg ctatttgatg tggatgatgg tcattagtac cagtctgagt
     6121 tcgtccatgt ttgtcaccgg tgcggcacca aacgtgctgg gtctggagtt cgtcagcaaa
     6181 attgccggta tccagattag ctggttgcag tggttcctct gcttcctgcc ggttggggtt
     6241 atcttgctta tcattgcgcc gtggctttcc tacgtgctgt acaaaccgga aatcacacac
     6301 agtgaagaag tggcaacctg ggcgggtgat gaactaaaaa ccatgggtgc gctgacacgc
     6361 agagagtgga cgctgattgg ccttgtattg ctcagcttag gtttgtgggt atttggcagt
     6421 gaagtcatta atgctactgc ggttggtctg ctggcagttt cgctaatgct ggccctgcac
     6481 gttgtgccgt ggaaagacat tacccgctat aacagcgcat ggaacacgct ggtcaacctg
     6541 gcaactctgg ttgtgatggc taacggcctg actcgttctg gttttattga ctggttcgcc
     6601 aataccatga gtacgcacct ggaaggattc tcaccaaacg caacggtgat tgtactggtt
     6661 ctggtgttct actttgcaca ctacctgttt gccagcctgt ctgcgcacac cgcaaccatg
     6721 ctgccggtta ttctggccgt cggtaaaggt attccgggcg taccaatgga acaactgtgt
     6781 atcctgctgg tgctatctat cggtatcatg ggctgtctga cgccgtatgc aaccggtcct
     6841 ggggtgatta tttacggctg tggctatgtg aaatcaaaag attactggcg tcttggcgca
     6901 atcttcgggg tgatttacat ctctatgttg ctgttggttg gctggccgat tctcgccatg
     6961 tggaactaat acgttcgcca aacgctgaat aaatcatcac gcccgccagg taaacccacc
     7021 tggcgggctt ttttatgatt aaatagataa tattcagtgt atgtatgagt tccacaccca
     7081 ttatgaaagc attctggcgt aacgccgcgt tgctcgcggt ttctctgctt cccttctctt
     7141 ctgccaacgc cgtggcgttg caggcaaaac agtatggcga ttttgatcgc tatgtcctgg
     7201 ccctctcctg gcaaaccgga ttttgccaga gtcaacacga tcgaaatcgt aacgaacgag
     7261 atgaatgtcg cctgcaaacc gaaacgacca acaaagctga ttttctgacc gtacatggtc
     7321 tgtggccagg attgcctaaa tcggttgctg cccgtggtgt tgatgaacgc cgctggatgc
     7381 gcttcggttg cgctactcgc ccaatcccga atctaccaga agcgcgcgcc agccgaatgt
     7441 gttcatcgcc ggaaaccggg ttatcactgg aaacggccgc taaactaagt gaagtcatgc
     7501 caggagctgg cggacgttcc tgcctggaac gctacgaata tgccaaacac ggtgcctgct
     7561 ttggttttga tccagacgca tacttcggca cgatggtacg cctgaatcaa gaaattaaag
     7621 agagcgaagc cggaaaattc cttgcggata actacggtaa aacagtgagc cgccgtgact
     7681 ttgatgccgc ctttgccaaa agctggggaa aagagaacgt gaaagcagtt aagctaacgt
     7741 gccagggtaa ccctgcgtat ttgactgaaa ttcagatctc gatcaaagct gacgccatca
     7801 acgctccgct ttctgccaac tcattcttgc cacaaccaca cccaggcaac tgtggcaaaa
     7861 cctttgtgat tgataaagca ggttattaat cctctgattt acaaaacacg ctaattcgtc
     7921 ttccataaac catgacgatt aagatttata cataatgcgc tacattgagg aattccctga
     7981 atgtgacgca aatcacttca aagagtgagc aacagtcagt atcatcatgt gagttaaacc
     8041 ctcgccgcct gacggtgagg gttttctttt gggtatgttt atctgcgaca ctcgcagtac
     8101 cgacgacatg gagtaaaaat gtccagacca actatcatca ttaacgacct ggatgccgaa
     8161 cgcatcgata ttctgctgga gcaacccgcc tatgctggtt tgccaatcgc cgacgcgtta
     8221 aacgcagagt tggatcgcgc ccaaatgtgt tcgccagaag agatgccaca cgacgtggtg
     8281 acaatgaaca gccgggttaa attccgcaat cttagcgatg gcgaagtgcg tgtgcgcacg
     8341 ctggtgtatc cggcaaaaat gaccgacagc aatactcagc tttccgttat ggctccggta
     8401 ggtgccgcac tgctggggct gcgcgttggc gattccattc actgggaact tccgggcggc
     8461 gttgcaaccc accttgaagt gctggaactc gaataccagc cagaagctgc tggcgactac
     8521 ctgctttaat cccatctgca caagcgataa ctcgctttgc ccagaggatg cactcgcatc
     8581 ctctttccgc tcaaatatca accagattta tcattttgct cttaagcgca gcattctaat
     8641 ctgaactggc tattcatctg cggaacctca tatgagcgaa gctctcggta tcactgcatt
     8701 ttatatattc atagcagcaa ttattgttgc tgcagtgcta taccttgaac aacgctggta
     8761 gcgcctggtt tttatttatt ttccagctca atagcaaatt cagatcaaat cagcctcgca
     8821 aattatagat tcataaccaa taatgtgctg ttatgtatac atgcaaaaac aaggagagaa
     8881 tgcttatgta caaaacaatc atcatgccag tggatgtttt cgaaatggaa ttgagcgaca
     8941 aagcgattcg ccacgctgaa ttcctcgccc aggatgacgg agttattcat ctacttcacg
     9001 tactaccggg gtcagccagc ctgagcctgc accgttttgc cgctgatgta cgtcgttttg
     9061 aagagcatct gcaacatgaa gcagaagaac gtctgcaaac gatggtcagc cacttcacca
     9121 tcgatccttc ccgcattaag caacatgtcc gttttggtag tgtgcgggat gaagttaacg
     9181 agttagcgaa agaacttgat gcagatgtag tagtgattgg ttcgcgtaac ccatcaattt
     9241 caactcatct gttgggttct aacgcctcga gcgtgatccg ccacgccaat ttgccggtgc
     9301 tggttgtgcg ttaattgcca gaataagtct cccgccctgc ccggagccat ccctgcaggg
     9361 ctttttaatg caataaaaaa gccgccaggt ttgaccccgg cggctaagca attgcaggtg
     9421 aatcttactt cttcttatgc agttttggtg cgaatcaggt agtcaaaagc actcagagag
     9481 gctttggcac cttcgccagt ggcgatgatg atctgcttgt acggaaccgt tgtacagtca
     9541 cccgctgcga acacgccttt cacgttggtt tcgcatttcg catcaatgat aatctcgccc
     9601 atgcggttac gttcgactgc gccttccagc cagttggtgt tcggcagcag accaatctgc
     9661 acgaaaatac cggccagttc gatgttgtga atatcgccgc tgacacgatc tcgatattcc
     9721 agacctacga ctttgctacc gtcgcctttc acttccgtgg tttgcgcatt cagaataatg
     9781 tcgacgtttt tcaggctgcg cagtttgtcc tgcagaacct ggtcggcttt catttctggc
     9841 gcaaattcca gcagcgttac gtgctcaacg atacccgcca ggtcaattgc cgcttccacg
     9901 ccggagttac cgccgccgat aaccgctacg cgtttacctt taaacagcgg gccgtcgcag
     9961 tgcgggcagt aggtcacgcc tttggtgcga tactgatctt cgcccggaac gttcatgttg
    10021 cgccattttg caccggtcgc cacgataatg ctgcgtgctt tcagtaccgc gccagaagct
    10081 gtttcaatct gatgcaggcc accttcaacc gctgccggga tcagtttgct ggcgctctgg
    10141 ctgtcgatca catcaacgtc gtattcatca acgtgaactt tcagcgctcc tgccagtttc
    10201 tggccttcgg tcttcggtac agaaatgtag ttttcgatat caacggtatc gaggatctga
    10261 ccaccaaaac gttcgcccat caaaccagta cggatacctt tacgtgcgga gtaaattgct
    10321 gccgctgcac ccgccgggcc ggaaccgacg attaatacgt cataagcatc acgcttgttc
    10381 agctcttctg ccgcacgttt ttccgcgcca gtatcaattt tggcgacgat ttcagtcaac
    10441 gtcatgcgac cctgaccaaa ctctttcccg tttacgaaca ctgccggaac gcccatcacg
    10501 ttgcgatcgg tgatttcgtt ctggaaggtg ccgccgtcaa ttgcagtgtg cttgatgcgc
    10561 gggttcagta cgctcatcag gttcagcgcc tgcaccacat ccgggcagtt gtggcaagag
    10621 agcgagtaat aggtttcgaa ttcaaaatca ccgtcaatat ggcgaatctg ctccagcaga
    10681 gactgcgctt ctttcgacgg atgaccaccg gtccacagca acgccagtac cagcgaggtg
    10741 aactcgtggc ccagcgggga gcctgcaaaa cgtggcccct ggttagaacc tgggttggtg
    10801 atcaggaaag acggcttacg caccggcaag ctgttatctt ctttaaaggt gactttgtct
    10861 gacagttctg cgatttcagc caacagttcc ttgatttctg ccgatttagc tctgtcatcc
    10921 agcgtggcaa ttaactcaac aggcttggtc aatttctcaa ggtaagcctt gagttgagtt
    10981 ttcatatttg tgtcgagcat gtttatctcc tgggctttaa acatcatcat gcaagcggcc
    11041 tctgcggcta cctggatgca gcttgcatca tggtgcgggg aagaaaatcg ggtgccgcag
    11101 cacccgaaga attaggtgaa tttccgaagt atttcgcgcc ggagttagta gcgcgaaaga
    11161 caaaggaaat ttagatttta ccaaccaggt ccagagacgg agccagagtt gcttcacctt
    11221 ctttccattt agccgggcaa acttcacctg ggtgagaagc tacgtactgt gctgctttga
    11281 ttttacgcag caggtcagac gcgtcacggc caatgccttc agcggtaact tcgattgcct
    11341 ggatgatacc ctgcgggtca acaacgaagg tcgcacggtc agccagacct tcatcttcac
    11401 gcatgttgtc gaagttacgg gtcagggcgc cagtcgggtc gccgatcatc gcatatttga
    11461 ttttagcgat ggtttcagag ctgctgtgcc atgctttgtg ggtgaagtga gtatcggtag
    11521 atactgcgta tacgtctacg cccagtttct gcaattcttc gtaatggtca gcaacgtcac
    11581 ccagttcggt cgggcatacg aaagtaaagt cagccgggta gaagaagaag acgctccagc
    11641 ggccttcggt atctttttcg gtgatttcga tgaattcgcc gtttttgaat gcctggtttt
    11701 taaaaggttt aattttggtg ttaatcaagg acatctatac ttcctccgtg ttttcgatga
    11761 gatgtaaggt aaccaatttt tgccgattcg gctaatgcgt ttccattatc aaatcgataa
    11821 gttttacctt acaaccttcg taagacaact ttgtgaactt tgcaccgcca gcggcgataa
    11881 aaagtaaaat ggcccccttt ccaggtgacc gtgtaatctg aactaccctt tggtatcttc
    11941 agcgcctgcc tgcaggctga ggtgttgccc ttacaaatgc aacaacgaca tggattacaa
    12001 caccctcata aacaaagggc aatcacctga tctaagctct tacctatgac agtgataggt
    12061 tatgcctttt actcgacttt tgcactgact gaaaaggaca aattaatgtt aaaaaagata
    12121 cttttactgg ctctgcttcc tgcaatcgcc ttcgcagagg aacttcctgc cccagtaaaa
    12181 gcgattgaaa aacagggtat tacaatcatc aaaacgttcg atgcccctgg aggaatgaaa
    12241 ggttatctcg gtaagtatca ggatatgggc gtcaccatct acctgactcc agatggtaag
    12301 cacgctatct ctggttacat gtacaacgaa aaaggtgaaa acctgagtaa cacgcttatc
    12361 gaaaaagaaa tttacgcacc cgccggacgt gaaatgtggc aacggatgga acaatcccac
    12421 tggctcctcg acggtaaaaa agatgcgccg gtcattgtct acgtcttcgc cgatccgttc
    12481 tgcccatatt gtaaacaatt ctggcagcag gctcgtccgt gggtagattc tggcaaagtg
    12541 caattaagaa cattgttggt tggggttatc aagccagaaa gcccggcgac agcagcggca
    12601 attcttgcct ccaaagatcc cgcaaaaacc tggcaacaat atgaagcctc tggtggcaag
    12661 cttaagctaa gcgtgcctgc aaacgtaagc acagagcaaa tgaaagtgtt aagcgacaac
    12721 gagaaactca tggacgatct gggggcaaat gtcacaccag ctatctatta catgagtaag
    12781 gaaaataccc tgcaacaagc cgtcggatta cccgatcaga aaatgcttaa tatcattatg
    12841 gggaataaat aaaggataat tcatggatgg ttgaaatata tccttgcttt tcaaccatcc
    12901 taaaaacgca tgaattgctc gtaacccacc tgacagctat gctattttag aaaatcttaa
    12961 tccaaag
//
LOCUS       DAPHCV010000123        12921 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.124, whole genome shotgun sequence.
ACCESSION   DAPHCV010000123 DAPHCV010000000
VERSION     DAPHCV010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.124"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(1..438)
                     /locus_tag="RH033_003855"
                     /pseudo
     CDS             complement(1..438)
                     /locus_tag="RH033_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            730..1251
                     /gene="fecI"
                     /locus_tag="RH033_003856"
     CDS             730..1251
                     /gene="fecI"
                     /locus_tag="RH033_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HDV2992977.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            1248..2201
                     /gene="fecR"
                     /locus_tag="RH033_003857"
     CDS             1248..2201
                     /gene="fecR"
                     /locus_tag="RH033_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HDV2992978.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            2288..4612
                     /gene="fecA"
                     /locus_tag="RH033_003858"
     CDS             2288..4612
                     /gene="fecA"
                     /locus_tag="RH033_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HDV2992979.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYLNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWDARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYNDNNKGIYAGQPRTLYMQGSLKF"
     gene            4657..5559
                     /gene="fecB"
                     /locus_tag="RH033_003859"
     CDS             4657..5559
                     /gene="fecB"
                     /locus_tag="RH033_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HDV2992980.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            5556..6554
                     /gene="fecC"
                     /locus_tag="RH033_003860"
     CDS             5556..6554
                     /gene="fecC"
                     /locus_tag="RH033_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HDV2992981.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVGWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVMLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            6551..7507
                     /gene="fecD"
                     /locus_tag="RH033_003861"
     CDS             6551..7507
                     /gene="fecD"
                     /locus_tag="RH033_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HDV2992982.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            7508..8275
                     /gene="fecE"
                     /locus_tag="RH033_003862"
     CDS             7508..8275
                     /gene="fecE"
                     /locus_tag="RH033_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HDV2992983.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(8833..9090)
                     /locus_tag="RH033_003863"
     CDS             complement(8833..9090)
                     /locus_tag="RH033_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992984.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(9173..10078)
                     /locus_tag="RH033_003864"
                     /pseudo
     CDS             complement(9173..10078)
                     /locus_tag="RH033_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(10088..11239)
                     /locus_tag="RH033_003865"
     CDS             complement(10088..11239)
                     /locus_tag="RH033_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
                     /protein_id="HDV2992985.1"
                     /translation="MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDT
                     GGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRR
                     YYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQK
                     TLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPI
                     HERSRNIDNRRSLGHWEGDLVSGTKNSHIATLVDRKSRYTIILRLRGKDSVSVNQALT
                     DKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKVYFCDPQSPWQRGTNENTNGLIR
                     QYFPKKTCLAQYIQHELDLVAAQLNNRPRKTLKFKTPKEIIERGVALTD"
     gene            11304..12209
                     /locus_tag="RH033_003866"
                     /pseudo
     CDS             11304..12209
                     /locus_tag="RH033_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc31 family transposase"
     gene            complement(12223..12921)
                     /locus_tag="RH033_003867"
                     /pseudo
     CDS             complement(12223..12921)
                     /locus_tag="RH033_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT         2847 a         3605 c         3508 g         2961 t
ORIGIN      
        1 tcgccatagt gcgttcaccg aatacgtgcg caacaaccgt cttccggagc ctgtcatacg
       61 cgtaaaacag ccagcgctgg cgcgatttag ccccgacata gccccactgt tcgtccattt
      121 ccgcgcagac gatgacgtca ctgcccggct gtatgcgcga ggttaccgac tgcggcctga
      181 gttttttaag tgacgtaaaa tcgtgttgag gccaacgccc ataatgcggg ctgttgcccg
      241 gcatccaacg ccattcatgg ccatatcaat gattttctgg tgcgtaccgg gttgagaagc
      301 ggtgtaagtg aactgcagtt gccatgtttt acggcagtga gagcagagat agcgctgatg
      361 tccggcagtg cttttgccgt tacgcaccac gccttcagta gcggagcagg aaggacatct
      421 gatggaaatg gaagccacgc aagcacctta aaatcaccat catacactaa atcagtaagt
      481 tggcagcatt accgaaatcc ccatccttta gggcagtgag gatgtcaata atagcagacc
      541 acatcatgag tcattctgac cgacacatgc cagaagcaga ggctccatcc cattttatac
      601 ctaccttata acacttagaa aaacaacatg ttaaaaatgt ctattggaaa caattttatt
      661 tccaattgta atgataacca ttctcatatt aatatgacta cgtgataatt aacttttgat
      721 gcactccgca tgtctgaccg cgccactacc acagcttcct taacgttcga gtcgctttat
      781 ggcacacatc acggctggtt gaaaagctgg ctgacgcgca aactccagtc tgcttttgat
      841 gcagatgaca ttgcccagga cacttttttg cgggtaatgg tcagcgaaac gctctcgacg
      901 atccgcgatc ctcgctcctt cctctgcact atcgccaaac gcgtgatggt ggacctgttt
      961 cgccgaaacg cgctggaaaa agcgtatctg gagatgctgg cgcttatgcc ggagggggga
     1021 gcgccttcac ctgaggaacg cgaaagccaa ctcgagaccc tacaactcct cgacagcatg
     1081 ctggacgggc taaacggcaa aacacgtgaa gcgtttctgc tttcgcaact ggatggtctg
     1141 acatacagcg agattgcgca caaactcggt gtttccatca gctccgtgaa aaaatacgtg
     1201 gcgaaagccg tcgagcactg cctgctgttc cgtctggagt atgggttatg aatcctttgt
     1261 taaccgattc ccgccgtcag gcgctgcgtt cagcttccca ctggtatgcc gtgctaagcg
     1321 gcgagcgcgt cagcccacaa caggaagcgc gctggcaaca gtggtatgaa caggatcagg
     1381 ataaccagtg ggcctggcag caggttgaaa acctgcgcaa ccagcttggc ggtgtgcctg
     1441 gcgacgttgc cagccgggcg ttgcacgata cccgcctcac ccgccgtcac gtgatgaaag
     1501 gattactgct gttgctcggc gctggcggag gctggcagct ctggcagtcg gaaaccggcg
     1561 aaggtctgcg ggcagattac cgcaccgcca aaggcacagt cagccgtcag caactggaag
     1621 atggctccct gctcacgctg aatacccaaa gcgcggcgga tgtgcgtttt gatgcgcatc
     1681 agcgcaccgt ccggctctgg tacggtgaaa tcgccattac taccgcgaaa gatgccctgc
     1741 aacgcccctt ccgtgtcctg acccgtcagg gccagctcac tgctttaggg acagaattta
     1801 ccgtccgcca gcaggataat ttcacgcagc ttgacgttca gcagcacgct gtggaagtgc
     1861 ttctcgccag tgcccccgcg caaaaacgca tcgtgaacgc tggtgaaagc ctgcagttca
     1921 gcgcctctga gtttggcgca gtgaaaccgc tggatgacga gagtacaagc tggacgaagg
     1981 acatcctgag cttcagcgat aaaccgctgg gtgaggtgat agccacgcta acccgttacc
     2041 gcaacggcgt gctgcgctgc gatcccgccg ttgccgggct gcgcctgagc gggacgttcc
     2101 cgctgaaaaa taccgatgcg atcctgaacg ttatcgcgca aacgcttccc gttaaaattc
     2161 agtctattac gcggtactgg ataaacattt caccactgta aggaaaataa ttcttatttt
     2221 gattgtcctt tttacccttc tcgttcgact catagctgaa cacaacaaaa atgatgatgg
     2281 ggaaggtatg acgccgttac gcgtttttcg taaaacaaca cctttggtta acgccattcg
     2341 cctgagcctg ctgccgctgg ccggtctctc gttttccgct tttgctgcac aggttaatat
     2401 cgcaccggga tcgctcgata aagcgctcaa tcagtatgcc gcacacagcg gatttaccct
     2461 ctcggttgac gccagcctga cgcgcggcaa gcagagcaac ggcctgcacg gcgattacga
     2521 cgtcgagagc ggcctgcaac agctgctgga cggcagcgga ctgcaggtaa aaccgctggg
     2581 aaataacagc tggacgctgg agcccgcgcc cgcaccaaaa gaagatgccc tgaccgtggt
     2641 cggcgactgg ctgggtgatg cgcgtgaaaa cgacgtattt gaacatgctg gcgcgcgtga
     2701 cgtgatccgc cgtgaggatt tcgccaaaac cggcgcaacc accatgcgtg aggtacttaa
     2761 ccgcatccct ggcgtcagcg cgccggaaaa caacggcacc ggcagccacg acctggcgat
     2821 gaactttggc atccggggcc tgaacccgcg cctcgccagc cgctcgaccg tcctgatgga
     2881 cggcatcccc gtccccttcg ccccttacgg tcagccgcag ctttcactgg ctcccgtttc
     2941 gctcggcaac atggatgcca ttgacgtggt acgcggtggt ggtgcggtgc gttacggacc
     3001 gcagagcgtg ggcggcgtgg tgaactttgt tacccgtgcc attccgcagg actttggtat
     3061 cgaggcgggc gtggaaggtc agctcagccc aacctcttca caaaacaacc cgaaagagac
     3121 gcacaacctg atggtgggcg gcacagcgga caacggtttt ggcaccgcgc tgctctactc
     3181 cggcacgcgc ggcagtgact ggcgcgagca cagcgccacc cgcatcgacg acctgatgct
     3241 gaaaagcaaa tatgcgccgg atgaggtgca caccttcaac agcctgctgc aatattacga
     3301 cggtgaagcc gacatgcccg gtggcctgtc tcgcgcggat tacgacgccg atcgctggca
     3361 atccacccgc ccgtatgacc gcttctgggg tcgtcgcaag ctggcgagcc tgggctacca
     3421 gttccagcca gacagccagc ataaattcaa cattcagggg ttctacaccc aaaccctgcg
     3481 cagcggctac ctggagcaag gcaaacgcat caccctctcg ccgcgtaact actgggtgcg
     3541 cggtattgag ccacgctaca gccagatctt tatgatcggc ccttccgcgc acgaagtggg
     3601 cgtgggctat cgctatttga atgaatcaac gcatgaaatg cgttactaca ccgccaccag
     3661 cagcgggcag ttgccgtccg gctcaagccc ttacgaccgc gatacgcgtt ccggcaccga
     3721 ggcgcacgcc tggtatctgg atgacaaaat cgacatcggc aactggacca tcacgccggg
     3781 tatgcgtttc gaacatatcg agtcatacca gaacaacgcc atcacaggca cgcacgaaga
     3841 agtgagctat aacgcaccgc ttccggcgtt gaacgtgctc tatcacctga ctgacagctg
     3901 gaatctttat gcaaacactg aaggctcgtt cggcaccgta cagtacagcc agattggcaa
     3961 ggctgtgcaa agcggcaatg tggaaccgga aaaagcgcga acctgggaac tcggtacccg
     4021 ctacgacgac ggcgcgctga cggcggaaat ggggctgttc ctgattaact ttaacaatca
     4081 gtacgactcc aaccagacca acgacaccgt cactgcacgt ggcaaaacgc gccataccgg
     4141 gctggaaacg caggcacgtt acgatctggg tacgctaacg ccaacgcttg ataacgtttc
     4201 catctacgcc agctatgcgt atgtgaacgc ggaaatccgc gagaaaggcg acacctacgg
     4261 caatctggta ccattctccc cgaaacataa aggcacgctg ggcgtggact acaagccagg
     4321 aaactggacg ttcaatctga acagcgattt ccagtccagc cagtttgcgg ataacgccaa
     4381 tacggtgaaa gagagcgccg acggcagtac cggccgcatt cccggcttca tgctctggga
     4441 cgcacgcgtg gcgtatgact ttggcccgca gatggcagat ctgaacctgg cattcggtgt
     4501 gaaaaacatc ttcgaccagg actacttcat ccgctcttat aacgacaaca acaaaggcat
     4561 ctatgcaggc cagccgcgca cgctgtatat gcaggggtcg ttgaagttct gatgtgttaa
     4621 cgcccggctt gccgggcttt tagctggaat gtgattatgt tggcatttat ccgttttctt
     4681 tttgcaggcc tgctgctggt gatcagccac gcctttgccg ccacggttca ggacgaacac
     4741 ggcacgttta cactcgaaaa aacgccacaa cggattgtgg tgctggaact ctcgttcgcc
     4801 gatgcgctgg ccgccgtgga cgtcagcccg atcggtattg ccgacgataa cgatgcaaaa
     4861 cgcatcctgc ccgaagtgcg tgcgcacctg aaaccgtggc agtccgtcgg aacgcgcgcg
     4921 cagccgagcc tggaagccat tgccgctctg aaaccagacc tgatcattgc cgacagcagt
     4981 cgccatgcgg gggtttacat cgccttgcag caaatcgcgc cggtactgct gcttaagtcc
     5041 cgcaacgaaa cctacgctga aaatttgcaa tctgcggcta tcatcggcga aatggtgggt
     5101 aaaaagcgag agatgcaggc acgtctggaa caacataaag agaggatggc gcagtgggcc
     5161 agccagcttc ccaaagggac acgcgtggcc tttggcacat cacgcgaaca gcaattcaac
     5221 ctgcatactc aggagacctg gaccggcagc gtgctggcct ctctggggct gaacgttccc
     5281 gctgcgatgg cgggtgcgtc catgccgtcc atcggcctgg agcaactgct ggcggtcaat
     5341 cctgcctggc tgctggttgc ccactatcgc gaagagagca ttgttaaacg ctggcaacaa
     5401 gatccgctct ggcagatgtt aaccgccgcg cagaagcagc aggttgcttc ggtcgacagt
     5461 aacacctggg cgcggatgcg cggtattttt gctgcagagc gtattgccgc tgacacggta
     5521 aaaatcttcc accatcagcc gcttaccgtt gtgaaatgac cgcgataaaa cacccggtgc
     5581 tgctgtgggg gcttcccgtt gcagcactta ttattatttt ctggctgagt ctgttttgct
     5641 actcggccat tcctgtttcc ggagcagatg caacccgcgc cctgctgcct ggacacacgc
     5701 caacgctacc agaagcgctg gtgcaaaacc ttcgtttgcc acgaagcctg gtcgccgttc
     5761 tgatcggcgc aagcctggcg ctcgcgggca cgctgctgca aaccctgacc cacaacccaa
     5821 tggcctctcc ttcactgctc ggcattaaca gcggcgcggc gctggctatg gcgcttacca
     5881 gcgcgctgag tccgacgccg attgcaggct attctctgtc gttcatcgcg gcatgcgggg
     5941 gcggcgtggg ctggctgctg gtcatgaccg caggaggcgg atttcgtcat acccatgaca
     6001 gaaacaaact gatcctcgcg ggtatcgcgc tgtcggcctt ttgtatgggc cttacccgca
     6061 tcaccctgct gctggccgaa gatcatgctt acggcatctt ttactggctg gcaggcggag
     6121 tgtcccacgc ccgctggcag gatgtctggc agctcttgcc ggtggtggtc actgcagtcc
     6181 ctgtcatgtt gctgctggcg aatcaactga acctgctcaa cctcagcgac agcaccgccc
     6241 atacgctggg agtgaacctg acgaggctac gtttggtcat caatatgtta gtgctgcttc
     6301 tggttggtgc gtgcgtcagc gtggcaggtc cggtggcgtt tatcggtctg ctggtgccac
     6361 atctggcgcg cttctgggca ggcttcgatc agcgcaacgt actgccagtg agcatgctgc
     6421 tgggggccac gctgatgctg ctggcagatg tactcgcacg cgcgctggcc ttccccggag
     6481 atctgcccgc aggcgcagtg ctggcgctga ttggcagccc ttgctttgtc tggcttgtga
     6541 ggaggcgagg atgaaaattg cgctggttat tttcatcacc cttgccctgg cgggctgtgc
     6601 gctgttatca ctccatatgg gagtgatccc cgtgccgtgg cgcgcgctgc tgaccgactg
     6661 gcaggccgga cgcgagcatt attatgtatt gatggagtac cgactgccgc gcttgctgct
     6721 ggcactgttt gtcggtgcag cccttgccgt agcgggcgtg ctgatacagg ggattgtgcg
     6781 caaccctctg gcatcaccgg atattctcgg cgttaaccat gccgccagcc tggcctctgt
     6841 gggggctcta cttcttatgc cgtcactgcc cgtgatggtg ctgccgctgc tggcctttgc
     6901 gggcggcatg gcggggttga tattgctgaa gatgctggca aagacccacc agccgatgaa
     6961 gctggcgctc accggcgtgg cgctttctgc atgctgggcc agcctgacgg attatctgat
     7021 gctctcgcgc ccgcaggatg tgaacaacgc cctgctgtgg ctgaccggca gcttatgggg
     7081 ccgtgactgg agctttgtga agattgccat cccgctgatg attttatttc tgccgctgag
     7141 cctgagtttt tgccgcgatc tcgacctcct tgcactcggc gatgcgcgcg ccaccacgct
     7201 cggtgtgtcg gtgccccata cccgattctg ggctttgtta ctagctgtcg ccatgacatc
     7261 taccggcgtg gccgcctgcg gcccgattag ctttattggt ctcgtggtgc cgcatatgat
     7321 gcgtagcatc accggtggac gtcaccgcag actgctgcct gtttcggccc tgacaggtgc
     7381 gttgctgttg gtggttgccg atctgctggc gagaattatt catcccccac tggagctccc
     7441 ggttggcgtg ctgaccgcca ttatcggtgc gccgtggttt gtctggttgc ttgtgagaat
     7501 gcgataaatg actttacgaa ctgaaaatct gacggtcagt tacgggacag acaaggtact
     7561 taacgacgtt tcactctcac tgccaacggg gaagatcacc gccctgatcg gtcctaacgg
     7621 ttgcgggaaa tcgacgctgt taaactgttt ttcgcggctt ttaatgccgc agtctggcac
     7681 cgtatttctc ggcgataatc ccataaatat gctctcatcg cgccagttgg cccgcaggct
     7741 ttcgctgctg cctcagcacc atttaacgcc agaggggatc acagtccagg agctggtttc
     7801 gtatggtcgt aatccctggc tgtcactctg ggggcgtctc tccgctgaag acaatgcacg
     7861 agttaatgtc gccatgaacc agacccgaat caatcatctt gccgttcgtc ggttaaccga
     7921 gctttccggc ggtcagcgcc agcgcgcatt tctggcgatg gtcctggccc agaatacgcc
     7981 cgttgtatta cttgatgagc caaccaccta tcttgatatc aatcaccagg tggacctgat
     8041 gcggttgatg ggcgaactcc ggactcaggg gaaaacggtg gtcgctgtgc tgcacgacct
     8101 taatcaggct agccggtact gcgatcaact ggtggtaatg gcaaacggac atgttatggc
     8161 gcaaggcaca ccagaagagg tgatgacccc aggattgctg agaacagtat tcagcgtgga
     8221 agcggaaata caccccgagc cggtatctgg cagaccgatg tgcctaatga ggtagattgc
     8281 acaggccgta agaaccaaac cacgactgaa tgaaactgga ctggcgccag caagcctgtt
     8341 cagactgggg ctgaactttt ccggactctg aaagattacc aatactcatc gtccatccgc
     8401 ttgctttagg ctgacaggtt cataatcaac gcaaaccaga gctgtacagg cttgggcgcg
     8461 gctttcaaac cagtcgtgat cacggcaatc aattttgaac tctgcttaac ggacatttct
     8521 gtataaccct tacggcaacg aaaaacgcga agttaaaatt ttagaaaccc aaaaacgtga
     8581 catgactaag tttagatttc agggggggag atcaaaaaat ttcgctctgt gccagagcgg
     8641 acattcacgg agctggttca ttaccaatga ggttgggctt ttgaggataa atcaatgatc
     8701 agacgccaac gtaaatcaaa agcaaccctg gaacggaatt gctaatccag tttctgacca
     8761 tcgatttttc taaaaagtgt gcgtttgcta ctacttaggt aggtgcagct ttcttaatca
     8821 ccggcagcca cgttatacag gccagttgat ggatcgattg ttatcaatga tatcttcatg
     8881 agtcggtgtc tcacccagct taccgaagct ggcataaagt gaaggcagac gggcccgtcc
     8941 ttctcccttt ttcgccagag ggaaagcgcg aagcatggtg gcagcctccc agtcacaaca
     9001 ataggatggt gtgcacggct gctgacgcca tgattcagcg atagagccgg aaaatacggg
     9061 gtcaaagccg gtatcattaa ccagagtcat cgtgacctgt actgcggctg gatgatctcc
     9121 tgctactgca actgctaggc gaccgcggct cccttcaggt agtctgttgt ggtcaactaa
     9181 aactggcctc cgcgttagag tttttccagt atcggttttc tgattcgttt ggtggtaacc
     9241 caccattata ttcgtgcggt cttagtgcgc tgtaatatcc aacgatatag tccgttattg
     9301 cgtgagctgc atcgctgaag cttacatagc ccgtcgctgg cacccattcg ttcttcagac
     9361 tcctgaagaa gcgctccatt gggctgttat cccagcagtt tccgcgccgg ctcatactct
     9421 gtctgatctg gtatcgccac aataactgcc ggaactgcct gctcgtataa tgactgccct
     9481 gatcgctgtg gaacatcacc ccgccgggct taccacgggt ttcccatgcc atttccagcg
     9541 ctttcatggt gagcctgctg tccggcgaga acgacatggc ccagcccact ggttttcttg
     9601 cgaacaggtc gagaacaacg gcgaggtacg cccagcgctt acccgtccag atataggtca
     9661 catcaccgca ccacacctga tttggctcgg tcacggcgaa ctgcctttca aggtagttag
     9721 ggatagcaac atgttcatga ccaccacgtt tataccggtg agtcggctgc tgacagctga
     9781 ccagccccag ctctttcatg agcctgccag caagccagcg tcccatctgg tagcctctcc
     9841 gggttgccat tgtggcgatg cttcttgctc cggcctaacc gtggctgatg ccatgtagct
     9901 caagtacctg actgcgtaat acagcccgtc tgcagtctgg tttttcagga cggtttttcc
     9961 agtatctgta gctgctgcga tgaaccccga acacatggca gagtgtgacc acaggataat
    10021 gcgctctgag tttcccgatt atcgagaact gttcagggaa tctgacatca agagcgcggt
    10081 tgtagattca atctgtcaat gcaacacccc tttcaattat ctctttcggt gttttgaact
    10141 tcagtgtctt tctcggtctg ttgtttagct gagcagcaac cagatctagt tcatgttgaa
    10201 tatattgggc aagacatgtc tttttaggaa agtactgccg aattagccca tttgtgttct
    10261 catttgttcc ccgctgccaa ggactctgag gatcgcagaa gtaaacttta acgccggtgc
    10321 tgacagtaaa ttctagatgt ctggccagtt ccattcctct gtcccatgtc agtgattttc
    10381 tgagttctga cggtaaactc aggaatttgt cggtaagagc ctgatttact gagacagaat
    10441 ctttgcccct gagtctaagg atgatcgtat aacgtgattt tcggtctaca agtgtggcta
    10501 tatgagagtt ttttgtacct gagactaaat cgccctccca atgtcccaga gagcgtctgt
    10561 tatcgatatt tcgggaacgt tcgtgaattg gtgttccgtt cactatgtta atcgtacctc
    10621 tttcgccttt gcgggtatga cgcctgccat ggcgaaggct atgcgaccgt cgcagatgct
    10681 gtatattcag gtggtgtagc gcttcacggc tacgaaagta cagcgtttta taaattgtct
    10741 caggtgatat tcgcagcgtt ttttgacgtg gttttgttcg ccttaaccat cctgatattt
    10801 gctctggaga ccatttcatc tccagctttt ccagaacaag ctttcgcaat ggtaaatttt
    10861 gatccagtaa gcacggtttt ggccttttcg ccattctgtt ggctcggtta ttagcatcaa
    10921 cagctttgta atagcgtctg ccccgattac gctgaacttc acgtgagatc gtcgaaggac
    10981 tgcgattcag cgcagtagct atcgcacgaa tgctcatttt ggctgacaaa ccagctcgta
    11041 tctcctcgcg ctcagacagt gtcaggtgag ctacagcccg cttacgctca tggggtttta
    11101 tgccgccagt atcccttaac atagtgaaga tcgttccggg ttttgaaccc aggatattcg
    11161 ctatttcact gaagcctgtt ccgttcttcc atagttcaaa aacagaggct ttttcctctg
    11221 ctgtaaatgt tcgtctcatt caaaaaacct ccgcaacccc atgttttcac ataactgttg
    11281 cgttgaccaa ttgaatctac aaccgcgctc ttgatgtccg actcgctgaa cggttcacga
    11341 tagttgccag actgagtgac agccactcag ttgtcagcct ctgttctgct ctggaaatac
    11401 accgcagcag ttaccggtac tggcgaaaac gacgcgatac ggttaatccg gcgcgagtca
    11461 ggttgtgcag cgaaatacgc cgggcgtgga accaaagtag gggctctgcg ggggcgcgca
    11521 cgctggctga aatgctgacc caaaacggcg tcccgatgag ccgttaccgt gccgggcgtc
    11581 tgatgaaata tctgaacctg agcagttgtc agcccggaaa acatcagtac aaaaatgctc
    11641 gtcaggaaca tacctgcctg ccgaatctgc ttgagcgcca gttcgctgtg cccgagccag
    11701 atagggtatg gtacggagat attacgtata tctgggcagg aaatcgctgg tgctatctgg
    11761 cggtcgtcat ggatcttttt gcccgcaggg ttatcggctg gagcctgtca gcgaatgccg
    11821 atactgccct gataagcagt gccctgcgga tggcgtatga ggtgcgtggt cagccgcggg
    11881 acgtcatgtt ccatagcgac cagggaagtc aatatacagg actgaaatat caacaacttc
    11941 tctggcgtta caggataaag caaagtgtca gccgacgggg aaactgctgg gataacagtc
    12001 caatggaacg cttcttccgc agtctgaaaa cagaatgggt gccaaccgat ggctacacag
    12061 gcaaagacgt ggcccggcag caaatcagca gttatattct gaattattac aatagcgtca
    12121 ggcctcacca ttataacgga gggctgacgc cggaagagtc agagaacaga taccattttt
    12181 actgtaaaac cgtggccagt attacttgac cactacaaac acccggtaac caccgtaagc
    12241 atcggcctga agcacaccgc tgtaaccggc caggtgattt tgtggatgga tacctttctg
    12301 gtccggactg tacgcgaacc agaccgccgg gggcatctgt gaaccggcgt tacggtcatc
    12361 acggacgtag acccacagcc gggctgtccg ggttttaccg ctgcccggct cctggaccgg
    12421 gacggggata tcatcagcat ggactttacc gggcatcagc acatactggc gcaggacgtc
    12481 atacagcggc tccagcagtt cagcaacagc actggtccag cgccccagcg tggcgcggct
    12541 cacctctaca ccctgacgac ggtatatttc tgactggcgg tataacggca gatggtctgc
    12601 atacttcccg gtgacgacat gggccagaag ccccgctccg gcatagctgc gtgcaatggg
    12661 ttttgaagac accggtgcct gcacgatatg gtcgcaccgg caacaggcca gtttcggacg
    12721 ttgtgtttcg ataaccttaa aggcgctgct gataagctcc agttgctctg acacatcaca
    12781 tcccagagaa ctgagttcgc caccacaggc cgggcagcat tcctcttccg gccggataac
    12841 ccgggtttca cgtggaagtg aggccggtaa cggtttacgg gctgaagact ggcgcagggc
    12901 ggatggcagt accggatcat a
//
LOCUS       DAPHCV010000124        12423 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.125, whole genome shotgun sequence.
ACCESSION   DAPHCV010000124 DAPHCV010000000
VERSION     DAPHCV010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.125"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            267..767
                     /gene="tssB"
                     /locus_tag="RH033_003868"
     CDS             267..767
                     /gene="tssB"
                     /locus_tag="RH033_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HDV2992986.1"
                     /translation="MADSFQNEVPAARVNIKLDLHTGNAKKKVELPLKLLAVGDYSNG
                     KEQRPLSERGKVDINKNNFNSVMAEFSPAVNLTVEDTLNGNGNEQNIALEFKSLKDFE
                     PEQVAKNIPQLRVLLAMRNLLRDLKSNLLDNATFRRELENILKDPTLSSELRDELAKI
                     APQENV"
     gene            826..2364
                     /gene="tssC"
                     /locus_tag="RH033_003869"
     CDS             826..2364
                     /gene="tssC"
                     /locus_tag="RH033_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HDV2992987.1"
                     /translation="MSVQQEHATSETATLTTTESGGVYQSLFDKINLTPVSSIQEIDL
                     WQNSETLADASPDERVTAAIHVLLSCLAKSDEDVVKLDKSLLDFHIDDLDQKISKQLD
                     AVMHHPEFQKVESLWRGTWFVVQRTDFRKNVKIELLDISKEHLRQDFDDSPEIIQSGL
                     YRHTYIQEYDTPGGEPVASLISSYEFDNSPQDIALLRNISRVSAASHMPFIGSVGPKF
                     FLKNSMEEVAAIKDIGNYFDRAEYIKWKSFRDTDDSRYVGLVMPRVLGRLPYGPDTVP
                     VRSFNYVEEVKGPDHEKYLWTNASFAFAANMVKSFVNNGWCVQIRGPQAGGAVADLPI
                     HLYDLGTGNQVKIPSEVMIPETREFEFANLGFIPLSYYKNRDYACFFSANSAQKPALY
                     DTADATANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRVLELELNTWIRTLVT
                     EMTDPGDELQASHPLRDGKVIVEDIEDNPGFFRVRLFAVPHFQIEGMDINLSLVSQMP
                     KAKA"
     gene            2384..3721
                     /gene="tssK"
                     /locus_tag="RH033_003870"
     CDS             2384..3721
                     /gene="tssK"
                     /locus_tag="RH033_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HDV2992988.1"
                     /translation="MKICRPLWAEGTFLSSQQFQQQARWEAFSNDSIAQLCIRHPWGI
                     ANVLFDRDALTPGKLKTQAVRLRFADGTLIDSDVADILPPACDLRTLEGGSAIVLLAL
                     PLAHGNGGNLGQGEQAERPLRYRQEWQKVQDIYGSDSEDMAVERHALSLRFAHDNNQD
                     YITCPLARLVRDVQGNWTQDESYIPPLLAFNAHDGLVQRLDTLLLQLRAKCQRLMAMR
                     RESNQRMADFAVADVSLFWLLNALNSAEPVLSDFLRYPAVHPELVWRELARLAGALLT
                     FSLEHNVSAVPPYVHESPSTVFPPLFSLLSELLEASLPSRVIALDLASLPGNRWKADL
                     HDPRLREEADFYLSVRSSLPAHQVLHQLPLVCKIGAPDDVTLLINVALNGVPLVPLTS
                     VPAALPLRLENQYFALDMHSDAAKSMLESGSCMIYAPGTMGDLKPELFAVLRT"
     gene            3718..4383
                     /gene="tssL"
                     /locus_tag="RH033_003871"
     CDS             3718..4383
                     /gene="tssL"
                     /locus_tag="RH033_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HDV2992989.1"
                     /translation="MKKDMDINIDALLRDTFLTVVELRQGTTVRHGIELYRHCQRQVE
                     LVRERLKDAGFSRENVEHITYAQCALLDETVLSRSGMDDGQAIWMKNPLQSHFFNTLQ
                     AGELLYERMKQVLQEPAPAQAVLTCFHRVLLLGFRGRYQDPAAPERDQLISTLNGQVA
                     PFGVLPETAVLNVPLSTRQHPLLHSPFFWLVTLALLLAGVWWGLHHWLNVLVDELLPQ
                     SLR"
     gene            4396..6048
                     /locus_tag="RH033_003872"
     CDS             4396..6048
                     /locus_tag="RH033_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDV2992990.1"
                     /translation="MRNTLKQAIVLWGMVLLLVLWSVFISPSGVLRWAGAAAIVLAVA
                     ALLIYRRRQAWTEMTGDAGLSSLPPETYRQPVVLVCGDMSAHLFTDSPVRQVSEGLYL
                     HVPDEEQLVAQVERLLTLRPAWASQLAVAYTIMPSIHRDVAVLAGRLRRFAHSMAIVR
                     RRAGVNVPWLLWSGLSGSPLPERANSPWFICTGGEVQVATSAETTMPAQWIAQSGAQE
                     RSQRLSYLLKAESLMQWLDLNVLAELNGPEAKCPPLAMAVGLVPSLPAVDNNLWQLWI
                     TARTGLTPDIADTGTDDALPFPDALLRRLPRQSGFTPLRRACVTMLGVTTVAGIAALC
                     LSATANRQLLRQVGDDLHRFYAVPVEEFITKARHLSVLKDDATMLDGYYREGEPLRLG
                     LGLYPGERIRQPVLRTIRDWRPPEQKMEVTASLQAQTVRLDSMSLFDVGQARLKDGST
                     KVLVDALVNIRAKPGWLILVAGYTDATGDEKSNQQLSLRRAEAVRNWMLQTSDIPATC
                     FAVQGLGESQPAATNDTPQGRAVNRRVEISLVPRSDACQDVK"
     gene            6106..6597
                     /locus_tag="RH033_003873"
     CDS             6106..6597
                     /locus_tag="RH033_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HDV2992991.1"
                     /translation="MAIPAYLWLKDDGGADIKGSVDVQGREGSIEVVALDHDVYIPTD
                     NNTGKLTGTRTHKPFTFTKEIDASSPYLYKAVTTGQTLKTAEFKFYRINDAGQEVEYF
                     NTTLDNVKLVRVAPLMHDIKDPSREKHNHLERIEFRYEKITWTYKDGNIIHSDSWNER
                     PSA"
     gene            6789..9414
                     /gene="tssH"
                     /locus_tag="RH033_003874"
                     /pseudo
     CDS             6789..9414
                     /gene="tssH"
                     /locus_tag="RH033_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520117.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
     gene            9426..11906
                     /gene="vgrG"
                     /locus_tag="RH033_003875"
     CDS             9426..11906
                     /gene="vgrG"
                     /locus_tag="RH033_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDV2992992.1"
                     /translation="MNVQFFDHAHHKLKIRGLQSPVDVLTFEGREQLSTPFRYDIQFT
                     SSDKAIAPESVLMQDGAFSLTAPPVQGMPVQTALRTLHGVITGFKHLSSSQDEARYEV
                     RLEPRMALLARSRQNAIYQNLTVPQIVEKILRERHQMRGQDFVFNLKSEYPAREQVMQ
                     YGEDDLTFISRLLSEVGIWFRFATDARLKIEVVEFYDDQSGYERGLTLPLRHPSGLFD
                     GETEAVWGLNTAYSVVEKSVTTRDYNYRTATAEMMTEQHDATGGDNTTYGEAYHYADN
                     FLQKGDKEAAESGAFYARIRHERYLNEQAILKGQSTSSLLMPGLEIRVQGDDAPAVFR
                     KGVLITGVTASAARDRSYELTFTAIPYSERYGYRPALIPRPVMGGTLPARVTSTVKND
                     IYAHIDKDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSIAF
                     EEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVSTEY
                     GGKSQLNLGHLVDAGKQQRGEGFELRTDMWGVVRAKKGIFISADAQDNAQGQVREMAP
                     AMAILNGAQSQMKSLSTDAQTANADPADLSSQIALLQQSVKDLNQAAILLSAPKGVAI
                     ASGEHLQLAAAKNVIANAGNNADVGVVKNMFIGVGQALSVFVRKAGIKLFANKGAISV
                     QAQNDLMELLAKKEIIITSTEDEIKITAKKKITLNGGGSYIRLDACGIEAGTPGEYNV
                     KAGYYGRKPKAKLTPELMAFPVIEGGDYSRCFKFFDDENIPYRNVKYIANFIDGTKKE
                     GVTDDEGCTETFFSDKEEEIEIEILLGV"
     gene            11939..>12423
                     /locus_tag="RH033_003876"
     CDS             11939..>12423
                     /locus_tag="RH033_003876"
                     /inference="COORDINATES: protein motif:HMM:NF013661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDV2992993.1"
                     /translation="MSKNVILSNGERENPSPLIYDKHKKHCIFRTKTLLSEEKKEDEK
                     CTVSVVFIDHEGYIRNAGIIKHTVPELEEGRMPKINAIILHRTDSSTINGTLASFSKG
                     IGAHFIVEKDGNIYQAASLFVYTSHVGKIKSRCKEEKTWSSEEKKKIEAFGWNAKKIH
                     D"
BASE COUNT         2963 a         3334 c         3546 g         2580 t
ORIGIN      
        1 taaaagtgca aatttgtgaa gcttatcctg ttattatttt aagtttcatg ccaaattctc
       61 tcaccagata atgccgccct cttccgaaaa ataatcaaga ggccaagcaa tatctaaaat
      121 gatacaactg tatctattcc cctgaaaaat acattattta tttgtatatt ttcctcatca
      181 ttgcttttta tttaaatcat ccgataatcc cctgaatata attatgttaa taaccatcag
      241 aaaaagtgga tgatgaggaa aaggatatgg ccgacagttt ccagaatgaa gttcccgccg
      301 ctcgtgtaaa tatcaagctt gatctgcata caggcaatgc aaaaaagaaa gttgaactcc
      361 ccctcaagct tcttgccgta ggcgattaca gtaacggaaa agagcaacgt ccgctgtccg
      421 aacggggcaa ggttgatatc aataaaaaca acttcaacag cgtcatggct gagttttcgc
      481 ctgcggttaa tttaacagta gaagatacgc taaacggaaa cggtaatgaa caaaatatcg
      541 cgcttgaatt taaaagcctg aaggattttg aacccgaaca ggtagcaaaa aatattccgc
      601 aacttcgtgt attactcgct atgcgcaatt tactacgtga cctgaaatcc aatctgctgg
      661 ataacgcaac gtttcgccgt gaactggaaa atatcctgaa agacccgacg ctcagttcag
      721 aactgcgtga tgaactggcg aaaattgcgc cgcaggagaa cgtttaaccc gaacgcgtat
      781 tcaggttaaa ccggagaaga aaggatcact gaaaaggaaa tgctgatgtc tgtacaacaa
      841 gaacatgcca cctctgaaac tgcaacactc accaccactg agtccggcgg cgtttatcag
      901 tccctgttcg ataaaattaa tttaaccccg gtctcttcca ttcaggaaat cgatttatgg
      961 caaaacagcg aaacgctggc tgatgcctca cccgatgagc gcgtgacagc agcgattcac
     1021 gttctgcttt cctgtctggc gaaatcagac gaggacgtgg tcaagctcga caagagcctg
     1081 ctggattttc atatcgacga tctggatcag aaaatcagta aacagcttga tgcggtcatg
     1141 caccaccctg aattccagaa agtcgagtcg ctgtggcgcg gcacatggtt cgtcgtacag
     1201 cgcactgatt ttcgcaaaaa tgtcaaaatt gaactgctgg atatcagtaa agaacatctg
     1261 cggcaggact ttgatgattc tccggaaatc attcaaagtg gtttatatcg tcatacatac
     1321 attcaggagt acgatacgcc gggtggcgaa cctgtagcct cattaatatc cagctatgaa
     1381 tttgataaca gcccgcagga tattgccctg ctgcgtaata tttccagagt gtctgccgct
     1441 tcccatatgc cttttatcgg ttctgtcgga ccgaaattct tccttaaaaa ttcgatggaa
     1501 gaagtcgccg cgattaaaga tatcggcaac tactttgacc gcgcagaata tattaaatgg
     1561 aagtcgttcc gcgatacgga tgacagccgc tatgtgggat tagtgatgcc gcgcgtgctg
     1621 ggccgtctgc cctatgggcc ggacacggtg ccggtacgca gctttaacta tgtggaagaa
     1681 gtcaaaggcc cggatcacga aaaatacctg tggacaaacg cctcgttcgc ctttgccgcc
     1741 aatatggtga agagctttgt gaataatggc tggtgcgtgc agatccgtgg cccacaggcg
     1801 ggcggcgcag tggccgatct gcccatccat ctttacgatc ttggcaccgg caatcaggtc
     1861 aaaattccgt ccgaagtgat gatcccggaa acccgcgagt ttgagttcgc caaccttggc
     1921 tttattccac tctcttatta caaaaaccgc gattacgcct gctttttctc ggcaaactcc
     1981 gcccagaaac cggcgttgta cgataccgct gacgccaccg ccaacagccg tatcaatgcc
     2041 cgtttgccct atatcttcct gctgtcccgc attgctcact acctgaaaat tattcagcgc
     2101 gagaatatcg gcactaccaa agaccgccgc gtgctggaac tggagcttaa cacctggatc
     2161 cgcacgctgg tgacggaaat gaccgatcca ggcgatgaac ttcaggcttc gcatccactg
     2221 cgcgacggga aagttatcgt cgaggacata gaggacaatc cgggcttctt ccgcgtcaga
     2281 ctctttgccg tgccgcattt ccagattgaa gggatggata tcaacctttc tctggtttcc
     2341 cagatgccaa aagcaaaagc ctgatcgggc ggtggtgtga tgtatgaaaa tttgtcgacc
     2401 actgtgggct gaggggacat tcctgtcttc tcagcagttt cagcagcagg cccgctggga
     2461 agcgttcagc aatgacagca ttgcgcagct ttgtattcgc catccgtggg ggatcgcaaa
     2521 cgtgctcttt gaccgggatg ccctgacgcc cggaaagctg aaaacgcagg cagtacgcct
     2581 gcgttttgct gatggcacgc tgatcgattc agacgtggcg gatattctgc cgcctgcctg
     2641 tgacctgcgc acgctggagg gcggcagcgc gattgttttg ctggcgctgc cgcttgcgca
     2701 tggtaacggc ggtaatctcg gtcagggcga gcaggctgaa aggccgctgc gctatcgtca
     2761 ggaatggcaa aaggtgcagg atatctacgg cagtgacagt gaagatatgg ccgttgagcg
     2821 ccatgcactg tccctgcgct ttgctcatga taacaatcag gattacatca cctgcccact
     2881 ggcgcgtctg gtgcgggatg ttcagggcaa ctggacacag gatgagagct atattccgcc
     2941 gttactggcg tttaacgccc atgacgggct ggtacagcgt ctggacacat tgctgttgca
     3001 gctacgggcg aagtgccagc gtctgatggc gatgcgccgg gaaagcaacc agcgtatggc
     3061 ggactttgcc gtcgccgatg tctccctgtt ctggctgctt aacgcgctga attccgccga
     3121 gccggtgctg agcgatttcc tgcgctaccc ggctgtccac cccgaactgg tctggcggga
     3181 gctggcgagg cttgccggtg cgctgctgac gttttccctt gagcataacg tcagcgccgt
     3241 tccgccctat gtccacgaat cgccgtccac cgtctttccg cccctgttct ccctgctcag
     3301 cgagctgctg gaagccagcc tgccgtcacg ggtgatcgcg ctggatctgg cgtccctgcc
     3361 gggcaaccgc tggaaagccg acctgcacga tccgcgcctg cgggaagagg ccgattttta
     3421 tctgtccgtt cgttcctcac tgcctgcgca tcaggtactg catcagctac cgctggtctg
     3481 caaaatcggt gcgcccgatg acgtcacgct gcttatcaac gtggcgctga acggtgttcc
     3541 actggtgccg cttacctcgg ttccggcggc gctgccgctg cggctggaga accagtattt
     3601 tgcgctggat atgcacagtg acgcggctaa atcgatgctg gagtccggga gctgcatgat
     3661 ttatgcgccg ggaaccatgg gcgacctgaa acctgaactg tttgcggtgc tgcgcacatg
     3721 aaaaaagata tggatatcaa tatcgatgcg ctgctgcgcg acacgtttct gacggtggtg
     3781 gaactgcgtc agggaaccac cgtgcgtcac ggtatcgagc tgtatcgcca ctgtcagcgg
     3841 caggtggaac tggtccgcga acgtctgaaa gatgccggat tcagtcggga gaacgtggag
     3901 cacataactt acgcgcagtg cgccctgctg gacgaaacag tgctcagccg tagtggcatg
     3961 gacgacggtc aggccatctg gatgaaaaac ccactccagt cgcatttctt caacaccctg
     4021 caggccggtg agctgctgta cgagcggatg aagcaggtgc tacaggagcc cgcccccgcg
     4081 caggccgtgc tgacctgctt tcaccgtgtc ctgctgctgg gatttcgcgg gcgttatcag
     4141 gatccggcag ccccggagcg cgaccagctt atttcgacgc tcaacgggca ggtcgcgcca
     4201 tttggtgtgc tgccggaaac ggcggtactg aacgtgccgt tatccacccg tcagcaccct
     4261 ttgctgcatt cgccgttttt ctggctggta acactggcgc tgctactggc gggcgtctgg
     4321 tgggggctac accactggct gaatgtactg gtggatgaac tgctgccgca aagcctgcgt
     4381 taaagggagt cgtgagtgag gaacacgctg aaacaggcca tcgtgctgtg gggaatggtg
     4441 ttactgctgg tgctgtggtc ggtgtttatc agtccgtctg gcgtgctgag atgggccggt
     4501 gcggcggcta tcgttctggc ggttgctgcg ttgttgattt accggcgcag gcaggcgtgg
     4561 acggagatga ccggcgatgc cgggttgtca tcgctgccgc cggaaaccta ccgacagccg
     4621 gtagtgctgg tctgtggtga tatgtcggcg cacctgttca ctgacagccc ggtccgccag
     4681 gtttcagaag ggctgtatct gcatgttcct gatgaagaac agcttgtggc gcaggtggag
     4741 cgattgctga cccttcgccc ggcgtgggca tcgcagcttg ccgtggcgta taccatcatg
     4801 cccagcatac accgggatgt ggcggttctg gccggacggc tgcgacggtt cgctcacagt
     4861 atggcgatag tgcgtcgccg ggcgggcgta aacgtaccct ggcttctctg gagcgggctg
     4921 tccggctcgc cgttgccgga gagagcgaat tcaccgtggt ttatctgtac cggcggcgaa
     4981 gttcaggtag caacatccgc agagaccacc atgcccgcgc agtggattgc acaatccggc
     5041 gcacaggaac gcagtcagcg actctcttat ctgctgaaag ccgaaagcct gatgcaatgg
     5101 ctggatctga atgtgctggc ggaactgaac ggcccggagg cgaaatgtcc accactggcg
     5161 atggccgtgg ggctggtccc ctcgttgcct gcggtggata acaacctatg gcagttgtgg
     5221 atcaccgcca gaaccggcct gacgccggat atcgcggaca ccggcacaga cgatgcgctg
     5281 ccattcccgg atgccctgtt acggcggttg ccgcgtcagt cgggctttac cccgctgcga
     5341 cgggcctgcg tgaccatgct gggcgtcacc accgtggcgg gtatcgccgc gctgtgcctg
     5401 tcagccacgg caaatcgcca gttattacgg caggtcggtg acgatctgca ccggttttat
     5461 gccgtcccgg tggaggaatt tatcaccaaa gcccgtcacc tgtcggtgct gaaagacgat
     5521 gcgaccatgc tcgatgggta ttaccgggaa ggagaaccac tgcgcctcgg tctggggtta
     5581 taccccggcg aacgcatccg ccagccggta ttacgcacca ttcgcgactg gcgtccgcct
     5641 gagcaaaaaa tggaagtgac ggcttcgctt caggcgcaga ccgtgcgtct tgacagtatg
     5701 tcgctgtttg acgtcggaca ggcccgcctg aaagacggct cgacaaaagt gctggtggac
     5761 gcactggtga atatccgggc aaaaccgggc tggcttatcc tcgtggccgg atataccgac
     5821 gccaccggcg atgaaaaaag caatcagcag ttatcgctgc gacgcgccga agcggtgcgc
     5881 aactggatgc tgcagaccag cgacatcccg gccacctgtt ttgccgtaca gggactgggc
     5941 gagagccagc ctgcggcgac caacgatacg ccacagggcc gggcagtcaa ccggcgcgtc
     6001 gaaatcagtc ttgttccgcg ttctgacgcc tgtcaggacg tgaaataaaa cataccgccg
     6061 gaagaaggcg gtgcttcaat cacactaaca aggagagtaa ttctcatggc tattcctgct
     6121 tatctctggc tgaaagatga cggcggcgcg gatatcaaag gttccgtgga cgttcagggg
     6181 cgcgaaggca gcatcgaagt ggtggcgctg gatcacgatg tgtacatccc gaccgacaac
     6241 aacaccggca aactgaccgg tacccgtact cacaagccct ttacgtttac caaagaaatc
     6301 gatgcgtcca gcccgtatct ctacaaggcc gtgaccaccg gccagaccct gaaaacggcg
     6361 gagtttaaat tctaccgcat caacgatgcc ggtcaggaag tggagtactt caacaccacg
     6421 ctggataacg tcaagctggt cagagtcgct ccgctgatgc acgacatcaa ggatccttcc
     6481 agagagaagc ataaccacct ggaacgtatt gagttccgct acgagaaaat cacctggact
     6541 tacaaagacg gcaacatcat tcattccgac tcgtggaatg agcgtccttc cgcctgataa
     6601 ctgcggcaga caggaaactg tctgcctttt tgttgtgtgc tttgtccagt gccgcaggca
     6661 ccggtactga gcaaagcata cccacaatct ggtgaaacct gatgggcctg tgtgaggtaa
     6721 cggctatggg cggtagcgtg cctgaacgcg gatcgcagga atacagaaca cgataaggaa
     6781 acccttctat gacaggaaat caccccgccg cgctgctgcg tcgccttaac ccatactgtg
     6841 cacgggcgtt ggacgctgcc gcctcactgt gtcagacccg cgcccatgcg gagataacca
     6901 ttgaacactg gctgctgaaa ctgctggagc agggagaagg cgatatcacg gtgattgccc
     6961 gtcgctatga atgggatatc gacacgctct ggcagtctct gctggcgcat ctggacacct
     7021 taccccgctc ggtccgcgaa cgtcctcaac tttctgaacc gctggcagcg cttatcagac
     7081 aggcgtggct gatagcgtca ctggaaggtg acgatccaca aatccgcagc cagcacctgc
     7141 tgatggcgct gacagaaaaa ccgatgctgc ccgcctgtaa tgacctgtgg gtattgctga
     7201 gtctgagccg cgtgcagctt gagcggctgc atcccctgct ggatgcgcag tcggatgaat
     7261 gtccggcacg tcagccacag gtcaccgaac cgctgacatc cgcgcaacca gagtcggcaa
     7321 cgacggacgt accggcaaaa acgctgacgg ggaaacagga tgacgccctg ctggcggtgc
     7381 ttaaccgctt taccgaagac gtgacggaaa aagcccgtag cgggcgaatc gacccagtat
     7441 tcgggcgcga caccgaaatt cgccagatgg tcgatatcct ctcccgtcgc cgcaaaaaca
     7501 acccgattct ggtgggagaa ccgggggtgg gcaaaaccgc actggtggaa gggctggcgc
     7561 tgcgtatcgc cgaaggcaac gtgcccgaca gcctgaaaac ggtgcatatc cgcacgctgg
     7621 acctcggtct gttacaggct ggcgcgggtg ttaaaggtga atttgagcag cgactgaaaa
     7681 acgtgattga tgcagcacag aaatcgccgg agccggtact gctgtttatt gatgaagccc
     7741 ataccattat cggcgcgggt aaccaggctg gcggcgcgga tgcggcgaac ctgctgaaac
     7801 cggcactggc aaggggcgaa ctgcgcacca tcgcggcgac cacgtggagc gaatacaaac
     7861 agtattttga gcgcgacgcc gcgctggagc gccgcttcca gatggttaag gttgacgagc
     7921 cggatgatga caccgcctgt ctgatgctgc ggggactgaa ggcccgttat gcacagcacc
     7981 acggcgtgca tatgctggac agcgccattc agaccgccgt gcgcctgtcg cgccgctatc
     8041 tgaccggacg ccagctgccg gacaaggcgg ttgatttgct ggataccgcc ggagcaagag
     8101 tccgcatgag cctcgacacc ctgccggagc cgctgacgca gcttcatgcg cgactggcgg
     8161 cgctagatat cgagcgggag gcgattcgtg ttttatcccg aaaccagccc ggagcggctg
     8221 gcagaactga ccgatttgcg tgatgagctg caggcagaag ccgggcatct ggaagcgcag
     8281 tatcagcagg aaaaggcact ggtgcagcag attatgacgt tgcgtcagga aggaacagac
     8341 agctctgaac tgcaacagca actgcggacg catcagggct ttgcaccgct gctggcgctg
     8401 gatgtggacg cccgcgccgt cgccacggtg gtggcggact ggactggcat cccgctctct
     8461 tccctgctca aggacgagca gagctacctg ctcagtatgg aacagagcct tgaaaaccgc
     8521 gttgtcgggc aacgcccggc gctctgcgcc atcgcacagc ggttgcgggc ggctaaaacc
     8581 ggcctcacgc cggagaacgg cccacagggg gtattcctgc tgaccggccc cagcggcacc
     8641 ggtaaaaccg aaactgcgct cacactggcc gacaccctgt ttggcggtga aaaatctctt
     8701 atcaccatca acctctccga atatcaggag ccgcataccg tctcccagct taaggggtct
     8761 cctccgggct acgttggcta cggtcagggc ggcgtactta ccaaagccgt tcgcaaacgc
     8821 ccgtacagcg tggtgttgct cgacgaagtg gaaaaggcgc accgcgacgt aatgaacctg
     8881 ttctatcagg tatttgaccg gggctttatg cgcgacggcg aaggccggga aattgacttc
     8941 cgcaacaccg tgattctgat gacggctaat ctgggcagcg accacatcat gcaactgctg
     9001 gaggaaaaac cggacgccac ggacgcagac ctgcatgaac tgctgtatcc ccttctgcga
     9061 gaccattttc agcctgcact gatggcgcgt tttcagacgg tgatttaccg cccgctggga
     9121 caggaagcga tgcgcgccat tgtggaaatg aaactggcgc aggtggttcg tcgtcttcgt
     9181 cagcactatg ggctggaaac ggaaatcaac gacagcctgt acgacgccct gaccgccgcc
     9241 tgcctgctgc cggacactgg ggcgcgtaat atcgacagcc tgctgaacca gcaaatcctg
     9301 ccggtattaa gccagcagtt gctggcgcag caggccgtgc atcataagcc tgcccgactg
     9361 cggcttggct gggataagga agacggcatt gtgctggaat ttgatgagaa ataatggagg
     9421 ctcttatgaa cgtacagttc ttcgaccacg cacaccacaa gctcaaaatt cgcggcctgc
     9481 aaagtccggt ggatgtgctg acctttgaag gccgcgagca actgagtacc ccgttccgct
     9541 acgacattca gtttaccagc agcgataaag ccatcgcgcc ggagtcggtg ctgatgcagg
     9601 atggcgcatt cagcctgacg gcaccgccgg tacagggaat gccggtacag actgccctgc
     9661 gcaccctgca cggcgtcatt accggcttta aacatctctc atcgtcacag gacgaagccc
     9721 gttacgaagt acggctggag ccgcgtatgg cgctgctggc ccgcagccgc cagaatgcca
     9781 tctaccagaa tctgacggtg ccgcaaatcg tcgaaaaaat cctgcgcgag cggcaccaga
     9841 tgcgcggtca ggacttcgtg tttaacctca aaagcgaata cccggcccgc gaacaggtga
     9901 tgcagtatgg cgaggacgac ctgacgttta tttcacgcct gctgtcggaa gtgggcatct
     9961 ggttccgctt cgccaccgac gcccggctga aaattgaggt tgtcgagttt tatgacgacc
    10021 agagcggcta tgagcgcggg ctgacgctgc cgcttcgcca tccttcaggg ctgtttgatg
    10081 gtgaaacgga agccgtatgg gggctgaaca ccgcgtacag cgtggtggaa aaaagcgtca
    10141 ccacgcggga ctacaactac cgcaccgcca cagcggagat gatgacagag cagcatgacg
    10201 ccaccggggg cgataacacc acctacggcg aagcctatca ctacgccgac aacttcctgc
    10261 aaaaaggcga taaagaggca gcggaaagcg gcgcgtttta tgcccgtatc cgccacgaac
    10321 gctacctgaa tgaacaggcc attctcaaag gccagagcac cagttcactg ctgatgccgg
    10381 ggctggaaat cagggttcag ggcgacgatg cgccagcggt gtttcgcaag ggcgtgctga
    10441 ttaccggcgt gacggccagc gcggcgcgtg accgcagcta tgaactgacg tttaccgcca
    10501 tcccgtactc agaacgctac ggctaccgcc cggcgcttat cccccgcccc gtaatgggcg
    10561 gcacgctccc ggcgcgggtc accagcacgg tgaagaacga tatttatgcc catatcgaca
    10621 aggacgggcg ctaccgggtc aacctcgact ttgaccgcga tacgtggaaa ccgggttatg
    10681 aaagcctgtg ggtacgccag tcgcgcccgt atgcgggaga cacctacggc ctgcacctgc
    10741 cgctgctggc gggaacggaa gtcagtatcg cctttgagga aggcaacccg gaccgacctt
    10801 atattgcagg cgtaaaacac gactcggcac acactgacca tgtgaccatt cagaactata
    10861 agcgtaacgt cctcagaacc ccggcgaaca acaaaatccg cctcgacgat gagcgcggca
    10921 aagaacatat caaggtcagt accgagtacg gcggcaagag ccagctaaac ctcggtcatc
    10981 tggtggatgc cgggaaacag caacgcggcg aaggctttga actgcgtacc gatatgtggg
    11041 gcgtggtgcg ggcaaagaaa ggcatcttta tcagcgccga tgcgcaggat aatgcgcagg
    11101 ggcaggtgcg cgaaatggct cctgcaatgg cgatactgaa tggcgcacag tcacagatga
    11161 agtctctgtc cactgatgcg cagacggcga atgccgatcc ggcagatctg tcatcgcaga
    11221 ttgcgttgtt gcaacagagc gtaaaagatc tgaaccaggc tgcgatcctg ctaagcgcac
    11281 ccaagggcgt ggcgatcgcc agcggcgagc acctgcaact ggcggcggca aagaatgtga
    11341 tagccaacgc aggcaacaac gccgatgtcg gcgtggtgaa aaatatgttt atcggcgtgg
    11401 gacaggcgct gagcgtgttt gtgcgtaaag cgggcatcaa gctgtttgcc aataagggcg
    11461 caatctcggt gcaggcgcag aacgacttga tggaactgtt ggcgaagaag gaaatcatca
    11521 ttaccagtac cgaagatgaa attaaaatca cggcgaagaa aaagatcacc cttaacggcg
    11581 gtggcagcta catccggctg gatgcctgtg ggatagaagc gggaacgccg ggagaatata
    11641 acgttaaggc gggatattat gggcgtaagc cgaaggcgaa gctgacgccg gagctgatgg
    11701 cgtttccggt gattgagggt ggagactatt ctcgctgttt taaatttttt gatgacgaaa
    11761 atatcccata tcgtaatgtt aagtatatag ccaattttat tgatggaaca aaaaaggaag
    11821 gtgtcactga tgatgaggga tgcaccgaaa cattttttag tgataaagaa gaagaaattg
    11881 aaattgagat tttacttggt gtttaaaaat accaccctta attattaaag agaaaaaaat
    11941 gagcaaaaat gtaatattaa gtaatgggga aagagaaaat ccttctccgc tgatctatga
    12001 caagcataaa aagcactgta tttttcgtac aaagacatta cttagtgaag agaaaaaaga
    12061 agacgaaaaa tgtactgtat cagttgtatt tattgatcat gaggggtata taagaaatgc
    12121 tggaattata aaacacacag tccctgaact tgaagaaggt cgcatgccta aaattaatgc
    12181 aataatattg catagaacag attcttcaac aataaatggt acacttgcta gtttttctaa
    12241 aggcattgga gctcatttta ttgttgaaaa agatggtaat atataccagg ctgcaagcct
    12301 atttgtatat accagccatg tggggaagat aaagtctcga tgtaaggagg aaaaaacatg
    12361 gtcgtctgaa gaaaaaaaga aaatagaggc attcggatgg aatgctaaga agatacatga
    12421 tca
//
LOCUS       DAPHCV010000125        12325 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.126, whole genome shotgun sequence.
ACCESSION   DAPHCV010000125 DAPHCV010000000
VERSION     DAPHCV010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.126"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            97..738
                     /locus_tag="RH033_003877"
     CDS             97..738
                     /locus_tag="RH033_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992994.1"
                     /translation="MFDLYQHIADNFLQKKAEDNLHVIYTGASIKYIESIFGPPVKEE
                     HSEDGRVHEYIYSFKKFYLQVVYDNNNTVILYSVTSKDENFHPEVPYLRKTLGHKFKD
                     FGSDIDYLQSSYSSKFYQYEEGHYLGNPGNYRNFYLAYNPAGVDYFELDPLPDLSNDD
                     KSPPNKNDLEKFRNNNAPNTFGVGDIHGGPEGLELSFGLGIDYYDARDIPDKD"
     gene            complement(856..1665)
                     /locus_tag="RH033_003878"
     CDS             complement(856..1665)
                     /locus_tag="RH033_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2992995.1"
                     /translation="MSLPVPFGAIVTVVKKCWSTLTQIMLIKTYQLSHERWAVRHRLG
                     ASWHSLGNYLEYSLRFAQLTDKEPRCSRIAFRSKEERLQQVELVFEAIGSGLRYQETL
                     CIHDVNNSPIILTLSNIPTLDVLILDDGFAFTVEQYQLKYCTIKLMTGESISIDNSPK
                     YSLMQNWCLNDTWKRRWGIIWNCNAIECARRRIAEYWKWGFCLPLVGRPPLYSPSRKG
                     IHLFEAKPLRWFMTRPWCLNIQFWTAIWSGLFILSDENVLQWRWKEPPQQD"
     gene            2148..4313
                     /locus_tag="RH033_003879"
     CDS             2148..4313
                     /locus_tag="RH033_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDV2992996.1"
                     /translation="MKTQITFAALLPALASFIPLHAHASSTSEDEMIVTGNTAADTTD
                     SAAGAGFKTNDIDVGPLGTKSWIETPYSSTTVTKEMIENQQAQSVSEMLKYSPSTQMQ
                     ARGGMDVGRPQSRGMQGSVVANSRLDGLNIVSTTAFPVEMLERMDVLNSLTGALYGPA
                     SPAGQFNFVAKRPTEETLRKVTLGYQSRSAFTGHADLGGHFDENKRFGYRVNLLDQEG
                     EGNVDDSTLRRKLVSVALDWNIQPGTQLQLDASHYEFIQKGYVGSFNYGPNVKLPSAP
                     NPKDKNLALSTAGNDLTTDTISTRLIHYFNDDWSMNAGVGWQQADRAMRSVSSKILNN
                     QGDISRSMKDSTAAGRFRVLSNTAGLNGHIDTGSIGHDLSLSTTGYVWSLYSAKGTGS
                     SYSWGTTNMYHPDAIDEQGDGKIRTGGPRYRSSVNTQQSVTLGDTVTFTPQWSAMFYL
                     SQSWLQTKNYDKHGNQTNQVDENGLSPNAALMYKITPNTMAYVSYADSLEQGGTAPTD
                     ESVKNAGQTLNPYRSKQYEVGLKSDIGEMNLGAALFRLERPFAYLDTDNVYKEQGNQV
                     NNGLELTAAGNVWQGLNIYSGVTFLDPKLKDTANASTSNKQVVGVPKVQANLLAEYSL
                     PSIPEWVYSANVHYTGKRAANDTNTSYASSYTTWDLGTRYTTKVSNVPTTFRVVVNNV
                     FDKHYWASIFPSGTDGDNGSPSAFIGGGREVRASVTFDF"
     gene            4324..5313
                     /locus_tag="RH033_003880"
     CDS             4324..5313
                     /locus_tag="RH033_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV2992997.1"
                     /translation="MKLSRLFPLLALLLAASLHAENTHKEVRIASPWPAQNTIIAMLG
                     YGDNIVGTSMIAKRIPLFRQSLPRIEKVAAVSVNSGHEINPEQIIALGVDMLFVPQNM
                     VVPQQALLKQAGVQVLAFEANSLRALTQRVQQTAAVLGPDAQQKALAYQRYFDRNVAL
                     VTGRLKDLPASQRVSLYHSMGNPLTTTGRPSLNQDWIDLAGGKNIAENWFGEHQQNRS
                     GEVALEKIVTANPAVIIAMNKRDADAILSSPQWASVDAVIHHRVYVNPKGMFWWCRET
                     SEEALQFLWLAKTLYPARFADVDIRKETREFYRQFFGLTLSDAQMSDVLNPPR"
     gene            5332..6390
                     /locus_tag="RH033_003881"
     CDS             5332..6390
                     /locus_tag="RH033_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDV2992998.1"
                     /translation="MTVLESTLSVENRYRQLFRQRLVILAVIFIAIIASLLLDFTLGP
                     SGLPLHSLIKTLMHPSGATNGMRVIVWDIRLPYALMALLVGMALGLAGAEMQTILNNS
                     LASPFTLGVSSAAAFGAALAIVLGIGIPGVPESGFIPANAFLFALLSALLLDSLTRWT
                     RVPTSGVVLFGIALVFTFNALVSIMQFVADEDTLQGLVFWTMGSLARASWEKLAVLAA
                     AMAIVLPWSLRRAWQLTALRLGEERAMSFGIDVRRLRLGSLLRISLLAALSVAFVGPI
                     GFIGLVAPHISRLLLGEDHRFYLPGSILIGGLVLSLASVASKNIIPGVILPVGIVTSL
                     VGVPFFLSIVMRHRGSMS"
     gene            6387..7154
                     /locus_tag="RH033_003882"
     CDS             6387..7154
                     /locus_tag="RH033_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV2992999.1"
                     /translation="MSGLTINALCAGYGKRRIIEHLSISTLPRGEVTVLLGPNGCGKS
                     TLLRALAGLNRASGEAWLNEENLLSLPFARRAEKVVFLPQSLPQGVHLQVLESVVVAQ
                     RASGAGQNQAQAIALLEELGIAHLAMNYLDSLSGGQKQLVGLAQSLIRRPALLLLDEP
                     LSALDLNYQFHVMDVVSRETRRRNMVTLVVLHDINIALRHAAQVIMLKEGKLIDSGDP
                     QTVIHAESLAQVYGVRGRVERCAQGRSMVIVDGAIEK"
     gene            7208..7465
                     /locus_tag="RH033_003883"
     CDS             7208..7465
                     /locus_tag="RH033_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001163787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993000.1"
                     /translation="MQHIDRLNVIKALVLLEDEQIVRFNIAANDNASQIHMLVDGLGV
                     GLTHEPVAAGMISHRTACHRSRQHDRPAHSYFYYLSAYQVA"
     gene            complement(7990..8201)
                     /locus_tag="RH033_003884"
                     /pseudo
     CDS             complement(7990..8201)
                     /locus_tag="RH033_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392653.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9440..9538)
                     /locus_tag="RH033_003885"
                     /pseudo
     CDS             complement(9440..9538)
                     /locus_tag="RH033_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516034.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(9998..10606)
                     /locus_tag="RH033_003886"
     CDS             complement(9998..10606)
                     /locus_tag="RH033_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993001.1"
                     /translation="MKMKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIHYFQN
                     LTPGWVSFNGEKPKIAIVPRSLHRLIYGSDKRATPPLDDDLVVNLCTSEHLLVHHPML
                     EGILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLDDWTENLK
                     RKSEKELGEWIIDRQKQNAALTDLMDQYVLLAYRTTVDGNRN"
     gene            complement(10694..10900)
                     /locus_tag="RH033_003887"
     CDS             complement(10694..10900)
                     /locus_tag="RH033_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDV2993002.1"
                     /translation="MTTPVSLMDDQMVDMTFITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            complement(11284..12075)
                     /locus_tag="RH033_003888"
     CDS             complement(11284..12075)
                     /locus_tag="RH033_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993003.1"
                     /translation="MTTRITFNMTSDETLRIVDEYCHTHKLSRSKVIDALLSATAPVL
                     NDINCYYQLAGKLQSRLLNGVYQRDLPHKRNVVSAEKYCLEIWENKLFTKRILEFDSS
                     NGVLYALKHKRHYRRDKMIGRVESRRIKDICEYQMQLSGEKAKYACFIYIERTIYNHD
                     NPSGGTPVKAAVGNAVILLAKDVIYDEYFFDLRQSFFVSVKDLMASGAKGIPETQKYP
                     DVYCWIPLFFINSGVVITPVYKIDPRKPVTVKKPDQITVVCNYRE"
BASE COUNT         2993 a         2988 c         3093 g         3251 t
ORIGIN      
        1 atgtcacatc cccctaaaaa acttaaaaaa tcattaatta ctgttttttt gagtggtatt
       61 cttgctgttc ttggatattt tgttgcattg aacggagtgt ttgatttata tcaacatatc
      121 gcagataatt ttttgcaaaa aaaagcagag gataatcttc atgtgattta caccggagcg
      181 tcaataaagt acatcgagtc aatttttggc cctcccgtga aggaagaaca ttctgaggat
      241 gggagggttc atgagtatat ttactctttc aaaaagttct atctgcaagt ggtttatgat
      301 aataataaca cagtgatact ctattccgta accagcaagg atgagaattt tcatcctgaa
      361 gtaccatatc tgagaaaaac ccttggacat aagttcaaag attttggaag tgatattgat
      421 tatttacagt cgagctattc ttcgaagttc tatcaatatg aagaagggca ttatttagga
      481 aatcctggca actatagaaa tttttacctt gcctataacc ctgctggagt tgattatttt
      541 gagttagatc cacttccaga tctctctaat gacgacaaat cgcctccaaa taaaaatgat
      601 cttgaaaaat tcagaaataa taatgctcca aatacatttg gggttggcga tatccacgga
      661 ggtcctgagg ggcttgagtt aagctttgga cttgggattg attattacga tgcgagagat
      721 attccagata aagattagtg atcatgtatc ataccatttt tatatcgttt caaattaggt
      781 agtcacaatt agactagtta atctaaccag cactgaggtc cgctcctcgc tcagagcgga
      841 cggccttcgt gaaagttagt cttgttgtgg tggctctttc cagcgccatt gcaagacatt
      901 ctcatcgctg agaataaaca gtcctgacca aattgccgtc caaaactgga tattcagaca
      961 ccatgggcgt gtcataaacc agcgtaacgg ttttgcttca aacagatgaa taccttttcg
     1021 cgatggtgaa tataacgggg ggcgtcccac taacggcaga caaaagcccc atttccaata
     1081 ttcagcaata cgccttcggg cacattcaat tgcattacag ttccagatga taccccaacg
     1141 ccgtttccat gtatcattga gacaccagtt ttgcattagc gaatatttgg gcgaattgtc
     1201 aatagatata ctttcaccag tcattaattt tatcgtgcaa tattttaact ggtactgttc
     1261 aaccgtaaat gcgaatccat catctagaat aagtacatca agtgtaggaa tatttgaaag
     1321 cgttaatatt atcggtgagt tatttacatc atgaatgcat agtgtttctt ggtagcgtaa
     1381 gccactgcca atggcttcaa aaacaagttc aacttgttgc agtcgttcct ctttgcttct
     1441 gaaagcaata cgtgaacaac gaggttcttt atctgtcaac tgagcaaatc gtagactgta
     1501 ctccaaataa ttccctaagg aatgccagct cgcccctagc ctgtgtctca ctgcccagcg
     1561 ctcatgtgag agttgataag tttttatcag cattatctgg gtaagagtag accagcattt
     1621 cttcactact gtaacaattg caccaaaagg aacaggaagt gacattacta aacccctaca
     1681 aatatcagat aattaggttt tatatcattt cagtcagtgt aatcaaatga taatcaggat
     1741 tggggctaat cttgaacgcc actaaagatc ggcaacaatg gggatccaat gtttcgttag
     1801 tgatttttcg cattagcaga tacatgctta cttccgctcc tcgctctcag cggaccttca
     1861 gctcagtgat atcgtccgct ctgtgccaaa ggcggacgct caagtagata ctgtcctgac
     1921 agtatgcggt aatcagagtt gttatatatt acttgcgtcc aaaccagctt tctgacaacc
     1981 cggccgctcc tgctttcaac aacgcgccgc catactttta tgctgcaaag agcggagaca
     2041 gaaggttagc gtaatttgta aataacttac attcacgtgt gatctggatc ataattttgc
     2101 ctatgcaccg atcgtaatat aaatatttac ataacgatca gtgattcatg aaaactcaaa
     2161 taactttcgc tgcgcttttg ccagcattag cgtctttcat accgcttcat gctcatgcct
     2221 cgtctacttc tgaagatgaa atgattgtca cgggcaacac cgccgccgac accaccgatt
     2281 ctgccgccgg tgccggtttc aaaacgaacg atatagatgt cggcccgctg ggaacgaaat
     2341 cctggatcga aacaccatat tccagcacca ctgtaactaa agagatgatt gaaaatcagc
     2401 aggcgcaaag cgtcagcgag atgctgaaat actctcccag tacgcaaatg caggcgcgcg
     2461 gtggaatgga tgtcgggcgt ccgcaaagtc gggggatgca gggcagcgtg gtggccaaca
     2521 gccgtctgga cgggctgaat atcgtttcaa caaccgcgtt tccggtggaa atgcttgagc
     2581 gcatggatgt gcttaacagt ttgaccggcg cgctgtacgg cccggcgagc ccagcagggc
     2641 agtttaattt cgtggcgaag cgcccaaccg aagagacgct gcgtaaagtg acgctgggct
     2701 atcaaagccg cagtgcgttt accggccatg ccgatctggg tggccatttt gatgaaaaca
     2761 aacggtttgg ctatcgcgtg aacctgcttg atcaggaagg ggaaggcaat gtggatgaca
     2821 gcacgctgcg tcgcaaactc gtttccgttg cgctcgactg gaatattcag ccgggcactc
     2881 agctacagct cgacgccagc cattacgaat ttatccagaa aggctatgtc ggtagcttta
     2941 actatgggcc gaacgtcaaa ctgccgtctg cgccgaatcc gaaggacaaa aatctggcgc
     3001 tcagcactgc gggcaacgac ctcactaccg ataccatcag cactcgcctg atccactact
     3061 ttaacgacga ctggtccatg aacgctggcg tgggctggca gcaggctgac cgcgcgatgc
     3121 gtagtgtttc cagtaaaata ctcaacaatc agggcgatat ctctcgttcg atgaaggatt
     3181 ccaccgctgc cggacgtttt cgcgtcctga gcaacaccgc cgggctgaat ggtcatattg
     3241 ataccggctc tatcggccac gatctgtcac tttctaccac gggatatgtc tggtcgcttt
     3301 atagtgccaa aggaacaggt tccagctata gctggggtac aacaaatatg tatcacccgg
     3361 atgcgataga tgagcagggc gatggcaaaa tccgcaccgg cgggccgcga taccgctcca
     3421 gcgtaaatac tcagcagagc gttacgctcg gcgatacggt gacatttacg ccgcagtggt
     3481 cggcaatgtt ctatctcagc cagagctggc tgcagactaa aaactacgat aagcacggta
     3541 atcaaacgaa ccaggttgat gaaaatggtt taagtccgaa cgccgcgctg atgtataaaa
     3601 ttacccctaa cacaatggcc tacgttagct atgccgattc gctggagcag ggcggtaccg
     3661 caccgacgga tgagagcgta aaaaatgccg gtcaaacgct aaacccgtat cgcagcaagc
     3721 agtatgaagt ggggctaaaa tcggacatcg gcgagatgaa tctaggcgcc gcgctgttcc
     3781 gactggaacg tccgtttgcc tatcttgata cggataacgt gtataaagag cagggtaacc
     3841 aggttaacaa cggccttgag ttaaccgctg ccgggaatgt gtggcagggg ctgaatattt
     3901 acagcggcgt gaccttcctc gacccgaaac tgaaagatac ggcgaatgcc tcaaccagca
     3961 ataaacaggt tgtcggcgtg ccgaaagtgc aggccaatct gttggcggaa tacagtttgc
     4021 cgtccatacc ggaatgggtt tacagcgcta acgtccatta tacgggcaaa cgcgcggcga
     4081 acgataccaa cacctcttac gccagcagct ataccacatg ggatttggga acgcgttaca
     4141 ccacgaaagt gagcaacgtc ccaaccactt tccgcgtggt ggtaaacaac gtgtttgata
     4201 aacattactg ggcttctatc ttcccatcgg gtaccgatgg cgataacggt tccccaagtg
     4261 cgtttatcgg cggcggccgc gaagtgcgtg catccgtcac cttcgatttc tgatggataa
     4321 atgatgaaac tctcccgttt atttccgctg ctggcgctgc tgctggcggc cagcctgcac
     4381 gcagaaaata cgcacaagga ggtgcgtatc gcgtcaccgt ggccggcgca aaataccatt
     4441 attgccatgc tggggtacgg cgataacatt gtcggaacct cgatgattgc aaaaaggatc
     4501 cccctgtttc gccagagcct gccgcgcata gagaaagtcg cagcagttag cgtaaacagc
     4561 gggcatgaaa tcaatcccga acagattatc gcgctggggg tcgatatgct ttttgtaccg
     4621 cagaatatgg tagtgcctca acaggcgttg ctgaaacagg ctggggtgca ggtgctggcg
     4681 tttgaggcga attcactgcg ggcgttaacg cagcgcgtac agcaaacggc agcggtgctg
     4741 gggcccgatg cgcagcaaaa agcgctcgcc tatcaacgct acttcgatcg caacgtggcc
     4801 ctggtcaccg ggcgcctgaa agatcttcct gcgtcacagc gcgtgtcgct ctatcacagt
     4861 atgggcaatc cgctgaccac caccggtaga ccctcgctaa accaggactg gattgacctg
     4921 gccggcggca aaaatatcgc tgaaaattgg tttggcgagc atcaacaaaa tcgttcgggt
     4981 gaagtcgcgc tggagaaaat tgtcactgcc aatccggcgg ttatcatcgc gatgaacaag
     5041 cgcgacgcgg atgccattct gagttctccg cagtgggcaa gcgtggacgc ggtcattcat
     5101 catcgcgttt acgttaatcc gaaaggaatg ttctggtggt gccgtgaaac cagcgaagag
     5161 gcgctgcaat ttttgtggct ggcgaaaacg ctttatcctg cgcgatttgc tgatgtggat
     5221 atacgcaaag agacgcgtga gttttatcgg cagttctttg gcctgacgtt aagcgatgcg
     5281 cagatgagcg atgtgctcaa tccgccgcgc taacggaggc tgtgaaaaac catgactgta
     5341 ttggaaagta cgctgtcggt cgagaaccgc tatcgccagc tttttcgcca gcgtctggta
     5401 atactggcgg ttatttttat cgctatcatt gcttcgctgc tgctcgattt caccctcggc
     5461 ccctcagggc tccctttaca ttcgctgata aagactttga tgcatccgtc cggggcgact
     5521 aacggcatgc gggttatcgt ttgggacatc cgtttgccct atgcgctgat ggcgctcctg
     5581 gtaggaatgg cgctgggttt ggccggggca gagatgcaaa ctattctgaa taactccctc
     5641 gccagcccgt ttacgcttgg cgtttcgtcg gcggcggcct ttggcgcagc gctggcgatt
     5701 gttctgggca tcggtattcc cggcgttccc gaaagcgggt ttataccggc aaacgctttt
     5761 ctttttgcgc tcctgtccgc cctgctgctc gatagtctga cccgctggac gcgcgtgccg
     5821 acgtcgggcg ttgtgctgtt tgggattgcg ctggtgttta ccttcaatgc gctggtgtcc
     5881 attatgcagt ttgtcgccga tgaagatact ttgcagggcc tggtgttctg gacgatgggc
     5941 agcctggctc gcgcatcatg ggagaaactg gcggtgctgg cggcggcgat ggcgattgtt
     6001 ttaccctggt cgctgcgtcg tgcctggcag cttaccgcgc tacgcctcgg cgaagagcgg
     6061 gcgatgagtt ttggcattga tgttcgccgt ttgcgcctgg gttccctgct gcgcatcagc
     6121 ctgctggcgg ccctttcggt tgcgtttgtg gggccaattg gctttattgg cctggttgca
     6181 ccgcatattt cccgcctgtt gctgggagaa gatcatcgct tttatttgcc cggaagcatc
     6241 cttatcggtg ggctggtgct atcgctggcc tcggtagcgt cgaagaacat cattcccggt
     6301 gtcattttac cggtaggaat tgttacctcg ctggtaggcg ttccgttctt tctgagcatt
     6361 gtaatgcgtc atcgagggag tatgtcatga gcgggcttac gattaatgcg ctgtgcgccg
     6421 gttacggcaa acggcggatt attgagcatc tgtcgatttc tacgctgccg cgcggcgaag
     6481 tcactgtact gctggggcct aatggctgcg gtaaatcaac gctactgcgc gccctggccg
     6541 ggctaaatcg cgccagtggc gaagcctggc tgaatgaaga gaatctctta tcgctgccgt
     6601 ttgctcgtcg ggctgaaaaa gttgtgttcc tgccgcagtc cctgccgcag ggcgtgcatt
     6661 tacaggtgct ggagtcggtg gttgtcgccc agcgcgcctc cggcgccggg caaaatcagg
     6721 cgcaggctat agcgctgctc gaagagctag gcatcgcaca tctggcaatg aactacctcg
     6781 atagcctgtc cggcggtcag aagcagctgg tggggcttgc acagtcgctt attcgccgcc
     6841 ccgcattatt attgctggat gaaccactga gcgcgctgga tctcaattat cagttccatg
     6901 tgatggatgt tgtatcgcgc gaaacgcgaa gacgcaatat ggttacgctg gtcgtcttac
     6961 acgatatcaa tatcgcactg cggcatgccg cccaggtcat catgctgaaa gaggggaaac
     7021 ttatcgacag cggcgacccg caaacggtga tccatgcaga gagccttgcg caggtatacg
     7081 gcgtacgcgg gagagttgaa cgttgtgctc agggaagatc gatggtgata gtggatggtg
     7141 caatcgaaaa atagtcggat taacgtggtt gcttcagcct caggtaaaga tcaataggaa
     7201 gtctgtaatg caacatatag accgccttaa tgtcattaaa gcacttgtgc ttctagaaga
     7261 tgagcagatt gttcgtttta acatcgctgc gaacgataac gcctcgcaga tccacatgct
     7321 cgttgacggt ctgggcgtcg ggctgacaca tgagcctgtc gctgccggaa tgatttcgca
     7381 ccggacagct tgtcaccgtt ctaggcaaca tgacagacct gcgcattcct atttctatta
     7441 tctatccgcc taccaagttg cttaatgtca ggctaaggcc ctttaatgac tggatttcag
     7501 accgattttc tggacaaccg agcgggtaaa tcgcagtaca ctgacagcta gacaataaag
     7561 ctccggttca tgtttgaacc atgtgtagtg aatgtccgct gttcgctcac aacggacctt
     7621 cagctcagcc gttccggccg cttcatgcca tgaatgtacg cagccagtca gaatattaag
     7681 gtgcggattt cagttcactt gccagcccag tgatacagga cagtaatgac caacaatttg
     7741 gtattgatac aacaagctgc ggccccggtt cgtgggggag atgaaacgaa ggtagctgaa
     7801 gttactgaaa tgaagaattc agagagactg aatgatatgc ctcaccttta gtgaatagtg
     7861 taaaacggga gactctcacc cgggattatc cactcatgct caatgtataa ttttcgtgcc
     7921 cctcacaggt aagagccgga tacattcgtt gtgtatgctg cagaattggt aaccggggcc
     7981 ctgcagtctt taactccgag tcttgtgctg ctcatttgcc acaaaatcct ggtacgccag
     8041 ctcaatcatt tcattcaaca gtttttttgg ggggcaccac gacttcgggc aatactttca
     8101 ggctgtttgt ggatttttcc ggacagtttc agcggaacgg aaccatcagc acgttcatca
     8161 cgcctttttt gttgctcccg ggcacttttc agttcatcca taatatagct gctcttctcg
     8221 catttttgcc gattttaaaa atgcgaacaa aacatccgta catgatgtac agattaaact
     8281 gccagggact ggtgtcagaa agcctccggt tagcctgctc tttccggaat gtgccggtta
     8341 tgaaaaaccg gaaattcagt ctctgttgta agatgtaccc tctgatacta cgttttcgtt
     8401 ttctctgtgt atttaactca ctgtatttat tgtcttttta agcctgactg atattctccg
     8461 gtcatagtgt gctggataga catcccgtaa ccttctcatt tttatataaa aatacctgca
     8521 ctgtcttcag cgcatctgtc tcctttaagt aaaaaatgtt ttcacgtatt gctgtaaatt
     8581 cattttaaat cagtatgtta tcctgatatt gctgtctcct ggtttgatgc gtaatcgttc
     8641 tgcctttctg gatgattctg gtgcttccgg gtgcattctc ctctgcacat gcaacagttc
     8701 gtcatcgcca cgtgcccaaa aagcatataa aaacgaatcg aatctgatcg ttttttcaat
     8761 cgtttttcac aggaatttcg tagggtgttc tccgctatag cctccatatt tgctgtcttt
     8821 ttcttcataa atcgtatgca atataaccag tctgtcaaga catcttccct gctgttgagg
     8881 tgacgatctc atttctggtc ttttccggga gagagacggt cagatgagcg agccggattt
     8941 actgccagat gtactccccg cggcagaaaa tagtgctcct gtttccgcaa tccgggatcg
     9001 gcaacgtccc atggttaatg ccacttgcag cgtcaggcac tgcaggttta cgtcgtgatc
     9061 tgatttcttc tgcacttatc ccgatcacgg aagttccccc tgcgcagaca tcggttcctg
     9121 ccgacatttc acaataccgc caggctgtca tgcagtaaca cccgcaatgc ggtctgcggt
     9181 tttaagcgca ctctcctgtt ttcagtgcac cgataagcgt tatagaaagt ctggggtgag
     9241 ttactgaatg tctgttaagt ggtgccgtgg gtggagactg cttacttttc cacagcaact
     9301 gcagtcattc aggatgctca ggagagaata tgtcaacgcc acgagccggg ccattttcat
     9361 gccagccagt atattttcta cactgatgca gtctgatttt caccattcca tcctcactga
     9421 ttgtcgtgtt gttttgggtc atcaccacag ctttccgttt ctcttttaat cggtaacgct
     9481 ctcctttgat attcagcgtg gttgagtgat gcagcagtca atccaggagc gctgttgtcc
     9541 ggcaaccacc atatcaactt cttcccggcg tgccggcgct ggtatccagg ccgggtttcg
     9601 aagcgaagcg ttcctgacgg actcattgac atccgggccg gatgtagtca tgcagcatgg
     9661 aacgtccgcc cgtatacact gacggcgtcc ggccgtgtac agagatcggt aaaattcgtt
     9721 actacaactg ttcctgaata tctgacgaag aaacattcag ccataacatc cggccgtgta
     9781 catctctttt gcggtgcagg gagtgctgtc gccatgtact tattgtcacc agggtgaggc
     9841 atgtaccgca gcatgccagc tcagcaatga caggatgccc ttaagacatc cgtcatggct
     9901 cagtatgatg acgacgaaca cgctctgcag gtgagcatgc agggccgggg gtatcatcat
     9961 ttccgtattt tgccgcacct tccggaaaca accggcatca gttacggtta ccgtcaaccg
    10021 ttgtgcggta tgccaggagc acgtactgat ccatcagatc cgtcagtgct gcgttctgct
    10081 tctgacggtc gatgatccat tcccccagct ctttttctga tttccgtttc aggttctccg
    10141 tccagtcgtc aaggctgttt ccggtcatta aatcaagcca gcaaagccag accagtttga
    10201 ggcgcaactc cgtgccacca aactgacgga agaggctgat cagtttattt gccagagaac
    10261 gctgcctcag acgttcacat tcagacagca gaatcccttc cagcatcgga tgatgaacca
    10321 gcagatgttc gctggtgcat aagttcacca ccagatcatc atccaggggc ggcgtggccc
    10381 gtttgtcaga gccataaatc agccggtgca gactccgggg tacgatggca atcttcggtt
    10441 tttcaccatt gaatgacacc cacccgggcg tcagattctg gaagtagtgg atattcggat
    10501 gacacacgcc ccaggatacg gcgtgccggg tgaagccggt cagccatttc tggtagcttt
    10561 cgtggagcag ctcgtaacgg gtctgatact gtggtttcat tttcatattc ctcacggggg
    10621 ataacaggca gcagagtgcc gccggcaggc cggagattgc gtctgctgtg ggcgaagggc
    10681 cagtggttaa aaattacgga cgggactgtg caatacgcgc ctgcagccag gcttccactt
    10741 cacttttcag ccagcgggag ctgcggccga gtttgatggg tgccggaaag gccccatcct
    10801 tgatgagctt gtaaaaccac ttatcggtca ggccggtcag ctgagtgata aacgtcatat
    10861 cgaccatctg gtcatccatc agcgaaactg gggtggtcat aaaaatctcc tccggatgtt
    10921 gttaatctca gccggcgttg atgatgaaaa caacgcacca ccttcaccgg cataacaacc
    10981 ggggagaaac gtaaaaaatc agtaggggaa tattcctgcc aggagagaaa ctgttgtgta
    11041 caggcaatac aggttgtctg ttttgtcagt gaagccggta taaacggaag atgattttgt
    11101 gttgccggaa tatgggatgg cgttttgccg tgacgggatc agtattttca gtgatgatta
    11161 ttactgtgca ggggagtacc ggagataaac cacgccctgt acctgccaga gcggaggttt
    11221 gtatttactg aacggaagat aaagcgaggt aaacactgtt gcggtgagcg gagaagactg
    11281 agattattct ctgtaattac aaacaacagt gatctgatcc ggttttttta ccgttacggg
    11341 tttccgggga tcaattttat agaccggggt aatgaccacg ccggaattta tgaagaaaag
    11401 cgggatccag caataaacgt ccggatattt ctgtgtctcc ggaatacctt ttgcgccaga
    11461 ggccatcaga tctttaacgc tcacgaaaaa tgactgccgc agatcaaaaa agtactcgtc
    11521 atatatcacg tcttttgcca gcaggatgac tgcattacca acagccgctt ttacaggtgt
    11581 cccaccggat ggattatcgt gattgtatat tgttctttca atataaataa aacaggcata
    11641 ttttgccttc tccccggaca gttgcatctg gtattcgcaa atgtctttga tacgccggct
    11701 ttcaacccgt ccaatcatct tatcccggcg atagtgtctt ttatgtttca gcgcgtacag
    11761 aacaccattt gaactgtcaa actccaggat tcttttggtg aaaagtttgt tttcccagat
    11821 ctccagacaa tacttctctg cactgacgac attgcgtttg tgaggaagat cccgttgata
    11881 cactccattt aataagcgtg actgcagttt gcctgcaagt tgataataac agttaatatc
    11941 gttaaggaca ggtgctgtgg cgctgagtaa tgcgtcgatt accttagagc gcgaaagttt
    12001 gtgggtatga cagtactcat ccacaattcg cagcgtctca tcactggtca tattgaaagt
    12061 tatacgtgtt gtcatgatga aattttctgt taatcatact ggttgatatg cataacattt
    12121 cataaaaaat acttttaatt catataattg attttttgac tggttttatc ctcgtgtggt
    12181 ggaaagtagt gttactgcca ctgtagtcag cataactcta tcacacagaa ccaccgaaaa
    12241 tgaagcattg ctcagggggg aagctgccac tcgtacgagt tttctgagca atgataacca
    12301 gtatttttcg ttttgggcga tttgt
//
LOCUS       DAPHCV010000126        12094 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.127, whole genome shotgun sequence.
ACCESSION   DAPHCV010000126 DAPHCV010000000
VERSION     DAPHCV010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.127"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            125..457
                     /gene="hdeA"
                     /locus_tag="RH033_003889"
     CDS             125..457
                     /gene="hdeA"
                     /locus_tag="RH033_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HDV2993004.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKKANFKDKVKGE
                     WDKIKKDM"
     gene            573..899
                     /gene="hdeB"
                     /locus_tag="RH033_003890"
     CDS             573..899
                     /gene="hdeB"
                     /locus_tag="RH033_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HDV2993005.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            963..1610
                     /locus_tag="RH033_003891"
     CDS             963..1610
                     /locus_tag="RH033_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDV2993006.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLGHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(1662..2432)
                     /locus_tag="RH033_003892"
     CDS             complement(1662..2432)
                     /locus_tag="RH033_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HDV2993007.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCVLHDLNLAARYADRIVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(2429..3385)
                     /gene="chuU"
                     /locus_tag="RH033_003893"
     CDS             complement(2429..3385)
                     /gene="chuU"
                     /locus_tag="RH033_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ChuU"
                     /protein_id="HDV2993008.1"
                     /translation="MGLSLLLLALVLFGASQGALKISFDALFDEEYRDIWLNIRLPRV
                     LLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIVLPFSFPVVLVLY
                     EQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAAIGILSYIGDEQQ
                     LRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQLGDEEAHYLGVNV
                     KRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRWLIPCSALAGACL
                     LLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            complement(3470..4093)
                     /gene="chuY"
                     /locus_tag="RH033_003894"
     CDS             complement(3470..4093)
                     /gene="chuY"
                     /locus_tag="RH033_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HDV2993009.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVVRHADAATKLAQ
                     QGVQVFTGDACDASVVADACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFVHRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            complement(4093..4587)
                     /gene="hutX"
                     /locus_tag="RH033_003895"
     CDS             complement(4093..4587)
                     /gene="hutX"
                     /locus_tag="RH033_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HDV2993010.1"
                     /translation="MSHVSLQEFLKTEPDGTLEVVAEQYNTTLLEVVKNLPSSTIVSG
                     DKFDTVWDTVCEWGNVTTLVHTADVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHALAASL
                     KEHA"
     gene            complement(4600..5937)
                     /locus_tag="RH033_003896"
     CDS             complement(4600..5937)
                     /locus_tag="RH033_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HDV2993011.1"
                     /translation="MNTNNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQT
                     WQEMINQTVSPRKRLVYLHIPFCATHCTFCGFYQNRFNDDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLPNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMMIMRNAERNAQWRHTLQSGVETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIVREKP"
     gene            complement(5957..6871)
                     /gene="shuT"
                     /locus_tag="RH033_003897"
     CDS             complement(5957..6871)
                     /gene="shuT"
                     /locus_tag="RH033_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ShuT"
                     /protein_id="HDV2993012.1"
                     /translation="MPRIITRPFLFSPLTLCISAVASAAKTAVKRKKLFTAVLALSWA
                     FSVTAAERIVVAGGSLTELIYAMGAGERVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLEQMYANIRQLAKTLQVP
                     EQGEALVTQINQRLERVQQNVAAKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
     gene            7555..9537
                     /gene="chuA"
                     /locus_tag="RH033_003898"
                     /gene_synonym="shuA"
     CDS             7555..9537
                     /gene="chuA"
                     /locus_tag="RH033_003898"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HDV2993013.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQAVEASESSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
     gene            9586..10614
                     /gene="chuS"
                     /locus_tag="RH033_003899"
     CDS             9586..10614
                     /gene="chuS"
                     /locus_tag="RH033_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HDV2993014.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNISEAELAFARVTHDAWR
                     MCGDIRDILAALESVGETKCICRNEYAVHEQVGAFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTTRGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFIADEN
                     MPLELKAVDAPVVQTRADASVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGYVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEVVTA"
     gene            complement(10675..11205)
                     /locus_tag="RH033_003900"
     CDS             complement(10675..11205)
                     /locus_tag="RH033_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV2993015.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQHAFVI
                     IDTLMNNVFHSNLLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEADVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(11358..11924)
                     /gene="slp"
                     /locus_tag="RH033_003901"
     CDS             complement(11358..11924)
                     /gene="slp"
                     /locus_tag="RH033_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HDV2993016.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         3143 a         2832 c         3000 g         3119 t
ORIGIN      
        1 tgaaataaaa atatctgatt ttgatatttt ccatcaacat gacatataca gaaaaccagg
       61 ttataacctc agtgtcgaaa ttgattcgtg acggctcttt cactttatag ttgaggatat
      121 tacgatgaaa aaagtattag gcgttattct tggtggtctg cttcttctgc cagttgtgag
      181 caatgcagcg gatgcgcaaa aagcagctga taacaaaaaa ccggtcaact cctggacctg
      241 tgaagatttc ctggctgtgg acgaatcctt ccagccaact gcagttggtt ttgctgaagc
      301 gctgaacaac aaagataaac cagaagatgc ggttttagat gttcagggta ttgcaaccgt
      361 aaccccagct atcgttcagg cttgtactca ggataaaaaa gccaacttta aagataaagt
      421 taaaggcgaa tgggacaaaa ttaagaaaga tatgtaattc cgggaatgcg ttacatcgta
      481 cttccttgca tattgaacag gccggaatat cttctttaaa agcagctatt cctcctgttc
      541 atatataatc tctatattga atgggttaca aaatgaatat ttcatctctc cgtaaagcgt
      601 ttatttttat gggcgctgta gcggctttgt cactggtgaa cgcacaatct gcgctggcag
      661 ccaatgaatc cgctaaagat atgacctgcc aggaatttat tgatctgaat ccaaaagcaa
      721 tgacaccggt tgcatggtgg atgctgcatg aagaaacagt atataaaggt ggcgatactg
      781 ttactttaaa tgaaaccgat ctcactcaaa ttcctaaagt gatcgaatac tgcaagaaaa
      841 acccgcagaa aaatttgtat accttcaaaa atcaagcatc taatgacttg ccgaattaat
      901 gaggtacaag taaaaaggag tagcaagttg agccatcttg ctgctccttt ttgcattttt
      961 atatgacagc agaatttatt atacgtctta tactcgcagc aattgcctgt ggcgctattg
     1021 gcatggaaag gcaaatgcgc ggcaaaggag cagggttacg cacacatgta ttaattggca
     1081 tgggaagcgc cctgtttatg attgtttcga aatatggttt tgctgacgtg ctgtctttag
     1141 gtcacgtcgg actggatccc agccgtattg ctgctcaggt tgtgacgggc gtcgggttta
     1201 tcggtgcagg taacattctc gttcgtaacc aaaatattgt aggtctgacg acggcagcgg
     1261 atatctgggt gaccgccgcc attggtatgg ttattggcag cggtatgtac gaactgggta
     1321 tttatggttc agtgatgacc ttgctggtgc tggaagtctt ccatcaatta accttccgcc
     1381 tgatgaacaa aaattaccat ttacagctga cattagtgaa cggtaatacg gtatcaatgc
     1441 tcgactggtt taagcagcaa aaaataaaaa cggatttagt ttcgttgcag gaaaatgaag
     1501 atcatgaagt ggtcgccatt gatatccagc ttcatgccac gacgtcgata gaagatctgt
     1561 tgcgattatt aaaaggtatg gcaggggtga aaggcgtttc aattagctaa tctgtatagt
     1621 tatttattcg caggagtacc agtgcggtac tcctgttggt tttaatgatc gaggacgatc
     1681 agtggtgaat ggttggcagg atctttaagt acggtaatat ccgcgccgta aagcatggtc
     1741 agcgcctgct gtgtcaatac gtcctgtggt ttaccatttg cgataacctt tcctttttgc
     1801 attaatacaa tacggtctgc ataacgcgcg gcaagattta gatcgtgcaa tacgcagcaa
     1861 acattgaact gccgttcgtg aactaactgg cgtaataaac ggaacaagtg ctgctgatgg
     1921 tgaatatcca gcgccgaagt tggttcatca agaaataacc atttcggtga gggagttggt
     1981 tcccagagtt gcaccagcag gcgcgctaat tgtacgcgct gttgttcacc accggataat
     2041 tgccgatagt cgcggtttgc taacgcctga cagtcacata acgccattat ttgtgctgtt
     2101 tcattatcct gattaccagt gcgatgcgga tgccgcccca tctggataac ttcttgtacg
     2161 ctgaatggaa acgccatgtg gctattttga cgcatcacgg cccggtgttt cgccagttca
     2221 gtaatcgacc attcatttaa tggcttatta aacaaccgac attcgccgct atcaggttga
     2281 agatagccgg ttaactggcg aagcagcgta gattttcccg caccattagg gccgagaatg
     2341 gcaacaattt ccccaccggg aaaagtcagc gagacattat cagtcagacg ccgcccctga
     2401 aggctataaa ccagattctg ggcgctgatc atgtgatcct ccggttgcgc agaatcaacc
     2461 acataaaata aggaccacca agcaggctgg ttaataatcc cacgggcatt tctgctggct
     2521 gtaccagcgt gcgggcaagc gtgtctgcca tcagcaataa acaggctccg gcgagggcgg
     2581 agcaagggat tagccagcgg tgatttgctc cggtagtcat gcgaatcaga tgcgggatca
     2641 ccaggccaat aaagccgata atgccgctta ccgatacggc ggcaccaacg agcagtgagc
     2701 tcactaacag taattgctgg cgtttacgct taacgttcac gccgaggtaa tgggcttctt
     2761 catcaccgag ctgcagtaaa ttcagcgttc cggcgagaca agttgttgcg ataatggccg
     2821 gtaggatgaa tgaactggca accaataacg tcggccattg cgcctgtcca agattgccca
     2881 tcatccacaa tgtgagttgt cgtagctgct gctcatcgcc gatatagctc aggatgccga
     2941 tcgctgcgcc gcacagggca ttgatggcga taccggcgag taataattgc atcatgctgc
     3001 catcgcgatg gcgctgcgtg atgagaaaaa tgatggtgca gaccactaaa cttccggcaa
     3061 tggcaaacac catttgctca tagagcacca gcaccactgg gaaggagaag ggcagcacaa
     3121 tagcaacgcc aaccattaat gcagaaccgc tactgacgcc aagtaatcca gggtcagcca
     3181 ttgggttgcg aaaaagcccc tgcataatta cccctgcggt tgccaacgct gcacctacca
     3241 gcaccgccag caaaacccgt ggtagacgaa tattgagcca gatatcgcgg tattcctcat
     3301 caaaaagggc atcaaaactg atctttaacg ctccctgact ggcaccgaac agcaccagag
     3361 ctagcaataa tagtgatagc cccataaaga ccctggcgga agagagtgaa tccttgagca
     3421 cgcaaaactc cgttacgaca ttacggcgcg gacatgccgc gccggggtat cacgccggtt
     3481 tcaggtccgg ttcaatcaga ctgtagacct gctggttaag tgctggcgca tttgccagtt
     3541 catggatatg tgcggcgaca tcggcacggt gaacaaaacc atgacactcc tgattttgta
     3601 tccgttgtgc tttgcccgtt gccgccccat ctagcagacc gccagggcgg agaatggcgt
     3661 aatcaagctg gctggtttgc aaccagcttt ccgccagcgt tttttcccgt accgcctgac
     3721 caaacgcagc ctttgctcgc tccgataaaa atggccagct atcgccgcat cccaaagagg
     3781 tcaccagaat catgcgactg atgccggcct tttcggcctc atcaatcact gttctgtgcg
     3841 ccagataatc ctgagctccg cccattgttg agatgataag cgcatccggg ccagctgcgc
     3901 ggcaagcatc cgcgactacg ctggcatcac aggcgtcgcc tgtaaaaacc tgtacgcctt
     3961 gttgcgccag tttcgtggcg gcatcggcat gacgaacgac agcaacgacc ggacgttgct
     4021 ctgccagcgc cagttcaagc gttctggcac ccacaccttt tccgcctgcg ccaaagagta
     4081 gccacggcgt catcaggcgt gttcctttaa cgatgctgcc agagcatgga aggcgctgac
     4141 ttgttcgctc aggagctgac gatggtcatc cctgccgagg aagattttca gcatggcgct
     4201 gccttcctta ttgaaaaaga gaatagaggc ggtgtccatg cccataaatt tacgttcgat
     4261 caaggcaata tgtgtgcagt tttctgcttt gatatgcccg gacatgccgt gctttccgcg
     4321 taggttgaaa tagccgtggc ggtggaaacc cgaaggcagt tcgccgctaa attcgaggat
     4381 tacatcggca gtatgtacta acgtggtgac gttgccccat tcgcagacgg tatcccagac
     4441 agtatcgaat ttatcgcctg acactatggt agaggagggc agatttttca ctacttccag
     4501 cagtgtggtg ttgtattgtt cggctaccac ctccagcgta ccgtccggct ccgttttcaa
     4561 aaattcttgt aacgagacat ggctcataaa tcactcctgt tatggttttt cacgcacaat
     4621 cgctggcgca ttaagtacct gaattagttc attaagagac tgcaaaatat tgcttgccca
     4681 gaaacgacct tcattagtca gccgcaggca agttgaagca tcgcgactta agcctttttg
     4741 gtgccattga gccagtaacg gcgcgagttt ttcggcatgt ggtgtaagtt catccagcgg
     4801 tacgcgcgct gtttcaacac ctgactgcaa ggtatgacgc cattgcgcat tacgttcggc
     4861 gttacgcatg atcatcatca gcggtttttt gcctgccgcg acggattcat gccaggtctg
     4921 aagattgcgt tcgttcatcc aggagtaacc attaatcgaa ccgccggctc cggaaccaaa
     4981 ggccagacaa tcggcacctt gtttaatcag caggttatag agattgcgtt cgcgtgtggt
     5041 acggccccag tggctgttac tgatgcagcg ccaaccggca tcgtccataa aatcacaccc
     5101 ttgcaggtaa agatcgcgac gttctgccgg agagggcacg gtagtacgcc cattttccac
     5161 ggctttgccc agcggtgtat tgggcaggac attgagcgca tagagatcga cgccgtcgag
     5221 accgatatcg cgggcaatag ccagatcttc tccccaggtt tgcgcatcct gacccggcag
     5281 accaaacagc aggtcacaga ccaccgcggc acggtcgcgt ttgaccaggc tttccataaa
     5341 cgcaatagcg gttgggccat ccgaggtgcg ggccattttc ttgcggattt tgctgttaaa
     5401 cgactgaata ccaattgaga agcggtttgc tccagcatca aggcaagcat cgattcgctc
     5461 agcgtcaaaa ttcagtaccc ggccttcaat agtgatttca caatcagggg ccagtggcag
     5521 cttttctcgt aatgtggtga tgattctggc caaatcgtgt gctgaaagcg cggaaggcgt
     5581 accgccaccg aaatagaccg cgtggatggg ggcagactga tgcaatacgc tgtctgcttc
     5641 catttctatt tcacgaatca gggcgtcggt ataatgagca cacgcatcgt cattaaaacg
     5701 gttctgataa aaaccacaaa acgtgcagtg tgtcgcacaa aaaggaatgt gtaggtaaac
     5761 cagtcgctta cgtggtgata ccgtttgatt gatcatctct tgccaggtct gtgccagttg
     5821 ctctttggca accggaatgg ccccacgaaa cggcatcatg gcgcgcctgt ctttaaaggg
     5881 ctgatcaccg tctaaagcaa aatgtggcgt gagatccagg gtgttatttg tgttcattcg
     5941 tctgatttaa atccatttat tgcggccaaa gctgctggtg taaagactca acgacatcag
     6001 caatacgcgg acccattccc agaatcaggt tttgatcaac ggtgataatg cgctggtttt
     6061 tccatgcggc agtatgggta attccggcaa tagaacgtag ccggttaata tcgccatcga
     6121 ccatttgtga ggtgacgaca atcacttctg gattagccgc aatcagtgat tctgcactgt
     6181 agcttttgta ctgctggtgg gtagcgacgt tttctgctcc ggcaagcgaa agaatggcgt
     6241 ccgcgacgct gcctttaccg gcaacctgtg gcgcacttcc gcctgcggag agaataaaca
     6301 tggctttaac cggggctttt ttggccgcca cgttttgctg tactcgctcc aggcgttggt
     6361 tgatctgtgt caccagcgcc tcgccttgtt caggaacctg taacgttttt gccagttggc
     6421 gaatgttggc gtacatctgc tcaagggtgg ctggtacacg cggcagagtg acgacgttga
     6481 ctttttgcgc ccgcagttgg tcgagcacaa tttgcggtcc agcatcctgc caggtaatta
     6541 cgctatccgg gcgaagtgac aaaatgcctt cgctgctgag ttgtttccag tagccaatat
     6601 gcggcagttt ggcggtttct ggtggataag aagtcgtttc atcgacacca accacgcgtt
     6661 cgccagcgcc catcgcgtag attagctccg tcagcgatcc tcctgcgacc acgatacgtt
     6721 cggcagccgt tacgctaaaa gcccagctaa gagccagtac tgccgtaaac aatttttttc
     6781 gttttaccgc ggtcttcgcg gcagaggcca cggctgaaat acataacgtc agtggggaaa
     6841 aaagaaacgg cctggtgatg atccttggca taatatgaga actcttatca aattgagaat
     6901 gatcatgatt atcatttgag ttatatatga aatacaaccg tcgattcaat acgcacggcg
     6961 ttacaagcgt atttattttt gatttttatc aagatgcttc gtcagctaac gttcttgtta
     7021 ccgactaaat ttcagcgcgg gaaaaagctg agaacccaga gatatcgcgg cttgcagagc
     7081 aaagagaaga gaaaattatc actgttaacg acaaagttta gtgatgaggt tatgggggaa
     7141 taaccataaa aacaaagtgg tttccagggc gcaggataag tttcataacc ccctgtttat
     7201 tgtgttaatg gcggttaaaa aaaagcttga tcataaaaaa ttgatcgata ataattctca
     7261 ttatcacgtt tgcatttaac aaaatacggc catatggata acaaagccat atcatttaca
     7321 aggggtaacg cacagcgctc ttcactcctg atggggcata tcggtttgcc tgacaaagag
     7381 ggcgtgcttt tgcgtatttg ccgagatacc aggcaatttc atgggatata aacgcctgta
     7441 aactcacccg gcgaatatta tcgctctgaa ttttaaataa atttattttt cgccagccac
     7501 cccggtatta acccaggcag ccagcatttt ttattttcct catggagaat cgctatgtca
     7561 cgtccgcaat ttacctcgtt gcgtttgagt ttgttggctt tggctgtttc tgccaccttg
     7621 ccaacgtttg cttttgctac tgaaaccatg accgttacgg caacggggaa tgcccgtagt
     7681 tccttcgaag cgcctatgat ggtcagcgtc atcgacactt ccgctcctga aaatcaaacg
     7741 gctacttcag ccaccgatct gctgcgtcat gttcctggaa ttactcttga tggtaccgga
     7801 cgaaccaacg gtcaggatgt aaatatgcgt ggctatgatc atcgcggcgt gctggttctt
     7861 gtcgatggtg ttcgccaggg aacggatacc ggacacctga atggcacttt tctcgatccg
     7921 gcgctgatca agcgtgttga gattgttcgc ggaccttcag cattactgta tggcagtggc
     7981 gcgctgggtg gagtgatctc ctacgatacg gtcgatgcaa aagatttatt gcaggaagga
     8041 caaagcagtg gttttcgtgt ctttggtact ggcggcacgg gggaccatag cctgggatta
     8101 ggcgcgagcg cgtttgggcg aactgaaaat ctggatggta ttgtggcctg gtccagtcgc
     8161 gatcggggtg atttacgcca gagcaatggt gaaaccgcgc cgaatgacga gtccattaat
     8221 aacatgctgg cgaaagggac ctggcaaatt gattcagccc agtctctgag cggtttagtg
     8281 cgttactaca acaacgacgc gcgtgaacca aaaaatccgc aggccgttga ggcttctgaa
     8341 agcagcaacc cgatggttga tcgttcaaca attcaacgcg atgcgcagct ttcttataaa
     8401 ctcgccccgc agggcaacga ctggttaaat gcagatgcaa aaatttactg gtcggaagtc
     8461 cgtattaatg cgcaaaacac ggggagttca ggcgagtatc gtgaacagat aacaaaagga
     8521 gcaaggctgg agaaccgttc cactctattt gccgacagtt tcgcttctca cttactgaca
     8581 tatggcggtg agtattatcg tcaggaacaa catccgggtg gcgcgacgac gggcttcccg
     8641 caagcaaaaa tcgattttag ctctggctgg ctacaagatg agatcacctt acgcgatctg
     8701 ccgattaccc tgcttggcgg aacccgctat gacagttatc gcggtagcag cgacggctac
     8761 aaagatgttg atgccgacaa atggtcatct cgtgcgggga tgactatcaa cccgaccaac
     8821 tggctgatgt tatttggctc atatgcccag gcattccgcg ccccgacgat gggcgaaatg
     8881 tataacgatt ctaagcactt ctcgattggt cgcttctata ccaactattg ggtgccaaac
     8941 ccgaacttac gtccggaaac taacgaaact caggagtacg gttttgggct gcgttttgat
     9001 gacctgatgt tgtccaatga tgccctggaa tttaaagcca gctactttga taccaaagcg
     9061 aaggattaca tctccacgac cgtcgatttc gcggcggcga cgactatgtc gtataacgtc
     9121 ccgaacgcca aaatctgggg ctgggatgtg atgacgaaat ataccactga tctgtttagc
     9181 cttgatgtgg cctataaccg aacccgcggc aaagacaccg ataccggcga atatatctcc
     9241 agtattaacc cggataccgt taccagcacc ctgaatattc cgatcgctca cagtggcttc
     9301 tctgttgggt gggttggtac gtttgccgat cgctcaacac atatcagcag cagttacagc
     9361 aaacaaccag gctatggcgt gaatgatttc tacgtcagtt atcaaggaca acaggcgctc
     9421 aaaggtatga ccactacttt ggtgttgggt aacgctttcg acaaagagta ctggtcgcca
     9481 caaggcatcc cacaggatgg tcgtaatgga aaaattttcg tgagttatca atggtaatca
     9541 tctgccccga tatttcgggg catttatctg gaaggaagag agacaatgaa ccactacaca
     9601 cgctggcttg agttaaaaga acaaaatccc ggaaagtacg cgcgtgacat cgcaggttta
     9661 atgaatatta gcgaagcaga actggcattt gcgcgcgtca cgcacgatgc gtggcggatg
     9721 tgcggcgata tccgtgacat tctggcggcg ctcgaaagtg ttggcgagac caaatgtatt
     9781 tgtcgtaatg aatatgcagt ccatgagcaa gttggtgcgt tcacaaacca gcatttgaac
     9841 ggacatgccg gattgatcct caatccacgc gcgctggatt tgcgtctgtt tctcaatcaa
     9901 tgggccagtg tttttcacat taaagaaaat acgactcgtg gcgaacgcca gagtattcag
     9961 ttctttgatc atcagggcga tgcattacta aaagtttatg ccaccgacaa taccgatatg
    10021 gcggcatgga gtgagcttct ggcacggttt atcgccgatg agaatatgcc gcttgagtta
    10081 aaagccgttg atgcgccagt tgttcaaacg cgagccgatg ccagtgtggt cgagcaagag
    10141 tggcgggcga tgaccgacgt tcatcagttt tttacgttgc tcaagcgcca caacctgacg
    10201 cgccaacagg cgttcaatct ggtggcagat gatttggcct gcaaagtatc caacagtgcg
    10261 ttggcgcaaa ttcttgaatc tgcacagcag gatggcaatg aaatcatggt gtttgttggc
    10321 aaccgtggct gcgtacagat tttcactggt gtggtagaaa aagtggtgcc aatgaaaggt
    10381 tggctgaata ttttcaaccc gacgtttact cttcatctat tagaagagag cattgctgaa
    10441 gcctgggtta cccgtaaacc gaccagcgat ggctacgtaa ccagtctgga attgtttgcc
    10501 catgatggta cgcagatagc gcaactttat ggtcaacgta cagaaggcga acaggagcaa
    10561 gcgcaatggc gtaagcaaat tgcttcgctg ataccggaag tcgttactgc ataacgtata
    10621 attaacggga ggtctggcta tttattgctg ggcctcttta tattaatcaa tcgatcacac
    10681 caaataatca atatgctgat ggcgtaccaa ctcattgatc cgcttaagat tgaggatcat
    10741 catgatattg tgtttatggc agtagaacgt ttttagcgat attttcagtt tctccagaat
    10801 atcttccgtt gtcatttgat tcgcaatgca gctcagaacc tgatgctgct tgcgcgaaag
    10861 ggacacatcc gcttcaggca cagagcagta actgccattg atgagtgcga caaagtcgat
    10921 gatggtgata gttcgcggaa caaaggtcac cggtactttc cccaggcagc gtttaatatt
    10981 aaagggagag aaaataatca catggacagg ttttaatcgt tcaatctgag tgagcaaatt
    11041 agaatgaaat acattattca ttaatgtatc aataatgacg aaggcatgct gatgcaacat
    11101 tacgtcgatc tcttcttcgt tctgtatatg aacgacatta cctttactca gaatgttttt
    11161 catcgcagtg aagaacatcg tatccctggt aattataaga aacataaccg actcctggtg
    11221 cggacattaa ctttataatc agtttgccaa aggtaattaa caggacaggg cttataataa
    11281 tagttagctg gctgtgtatt aatttgatgt aatgcatgat gcagatgctg agaaagcatc
    11341 tgcatctctc ggtggtgtta tttgaccagc tcaggtgtta cctgactcac cgcattggtg
    11401 tagtaaggcg cgccccaccc cggttccggc cagaatgcac catagccgta atcccacagg
    11461 ttataggtgg tattaaccac ttctctcaaa tgccacacct ggatgccctg catattcact
    11521 tccaggaagt tatacgggac tttattgata aagccaggtt gttcaccctg aatggtaccg
    11581 aggatggtaa caaagtgatt acgatagttc actggatcaa ggaagccgct ttgtctggcg
    11641 agcagtcggc cctgatagtt ggcttcaata tcaggcttcg catagctatc caacggtaat
    11701 acagcgattt ctaacaacgt atccgttttg ccattgataa cgttgataac cttcccacca
    11761 aagcgcgctt gttgaccaac atataacccc ggctggttat gaacagcaac aaaacttttt
    11821 tgaatatcag gttggttatt gcctttgata ttttgcggaa ttgaactaca tgcggcaagc
    11881 aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt tcatgttact atccttatca
    11941 acaaatatta aaaccataat agatatttct ttatagtttt catctgattc tgaggcgata
    12001 tgcctgatag taacgtaaac ctgtctgatg tttatatccc ttctgtttag tttaattctg
    12061 ttagttttgg ggcgtgacat gagtatccat cgcc
//
LOCUS       DAPHCV010000127        11778 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.128, whole genome shotgun sequence.
ACCESSION   DAPHCV010000127 DAPHCV010000000
VERSION     DAPHCV010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.128"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(293..505)
                     /gene="cspH"
                     /locus_tag="RH033_003902"
     CDS             complement(293..505)
                     /gene="cspH"
                     /locus_tag="RH033_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HDV2993017.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            791..1003
                     /gene="cspG"
                     /locus_tag="RH033_003903"
     CDS             791..1003
                     /gene="cspG"
                     /locus_tag="RH033_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HDV2993018.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            1014..1202
                     /locus_tag="RH033_003904"
     CDS             1014..1202
                     /locus_tag="RH033_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDV2993019.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            1177..1407
                     /gene="ymcE"
                     /locus_tag="RH033_003905"
     CDS             1177..1407
                     /gene="ymcE"
                     /locus_tag="RH033_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HDV2993020.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            1397..1570
                     /locus_tag="RH033_003906"
     CDS             1397..1570
                     /locus_tag="RH033_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HDV2993021.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1619..2692)
                     /locus_tag="RH033_003907"
     CDS             complement(1619..2692)
                     /locus_tag="RH033_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDV2993022.1"
                     /translation="MTEKRRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVTRPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQFVLPRWLDTPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKSCPSRIPVDKLIQVRSV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYQRLIPQSPMIGH"
     gene            complement(2764..5508)
                     /gene="torS"
                     /locus_tag="RH033_003908"
     CDS             complement(2764..5508)
                     /gene="torS"
                     /locus_tag="RH033_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HDV2993023.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQHQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRSQVATTLTTVSQYNDLLALY
                     QQDNEISNRLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKANQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGHIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLLLRIATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNSSGAQVVAVGNATQALETLQNSEPFAVALVDFDLPDID
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQPLDVSQLNEDAQLMGTEKIHEWLVLFSQHALPLLDEIDIARASQDNEKIK
                     CAAHQLKSSCSSLGMRSASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5591..6619
                     /gene="torT"
                     /locus_tag="RH033_003909"
     CDS             5591..6619
                     /gene="torT"
                     /locus_tag="RH033_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HDV2993024.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNAIDAPHVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVMTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6592..7284)
                     /gene="torR"
                     /locus_tag="RH033_003910"
     CDS             complement(6592..7284)
                     /gene="torR"
                     /locus_tag="RH033_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HDV2993025.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            7414..8586
                     /gene="torC"
                     /locus_tag="RH033_003911"
     CDS             7414..8586
                     /gene="torC"
                     /locus_tag="RH033_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HDV2993026.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            8586..11132
                     /gene="torA"
                     /locus_tag="RH033_003912"
     CDS             8586..11132
                     /gene="torA"
                     /locus_tag="RH033_003912"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HDV2993027.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKEGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKIHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGINAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPTSQVKS"
     gene            11129..11728
                     /gene="torD"
                     /locus_tag="RH033_003913"
     CDS             11129..11728
                     /gene="torD"
                     /locus_tag="RH033_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HDV2993028.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNGLENSIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLDLLSHLHFSLGEGTVPARRIDGLRQKTLTA
                     LREWLPEFAARCRQYDSFGFYAALSQLLLVLVECDYQKR"
BASE COUNT         2902 a         2948 c         3089 g         2839 t
ORIGIN      
        1 agattgttaa ctattcccct ctggaaatac gacttgtgag cataagataa atcttagcaa
       61 aatacaaatt aaagataaga aaattgttaa gaaagctatt aactattgtt ggtaatatag
      121 tttcatgtgg tactataact ggctgttaag tggcatttat aactattagg tgcagagata
      181 ttcgcttaaa tggattagtt tctttgtgaa aaagtaactt gcgttataat gtgtagtgtt
      241 gactattgag ctattccaat aaaaccagtc agctttaaac aagcaactca tattaagaga
      301 gatatacatt tgccgctgtt ggtcctcgca ggccatttac gcggcaaaat tccacgcgta
      361 atcctggtat aagcacttct gcgtcgcggg gagtgaatgc ggaaatatgg acctggactt
      421 ctttacgacc gtcggagggg ataatgaatc ctttgccgct tttgcgatca aaggttttga
      481 caattcctgt cattttacgg gacaaacaaa ttccttactg aaaatactgc gctgcactat
      541 acggggttaa taaaataaag ccagcgatat ttaagaccgc cggacggcta aaataaaatt
      601 tgcttaatct caattatcat gcgttaatag ctgcgtcggt ttgaaagaca gacagcatac
      661 aaagtagttt actaaagcag ttctcattat caggcattat ccccttcttt tgagtctctc
      721 tcctgaacac taagtagttt ctgtattaaa gccctgtttg ccgaaaggcc caaaatgaag
      781 gaagtaaaat atgtctaata aaatgactgg tttagtaaaa tggtttaacg cagataaagg
      841 ttttggcttt atcactcctg atgatggcag caaagacgtt ttcgtccatt tcaccgccat
      901 tcagagcaat gaattccgca cactgaacga aaatcagaaa gttgaatttt ctattgagca
      961 ggggcaacgt ggccccgcgg cagcgaacgt tgttacgctc taaggttgcc gttattactc
     1021 aacatctcca tttccgctgt ccatgttgtc atggttcaca gtaccgcaca tcggcattcg
     1081 atgtgacgga gcgaaaccct ttgggcgcta agtgtatttt ttgtaaatcg acgatgatca
     1141 cctttgataa cgtcgcgctg caaatacgca ctgaccatgc gccgctggat ttcacaaaat
     1201 aatatcaggc tccctcgtgg agcctttttt atatctgcct tatttttctt caacgctgta
     1261 tgtatagtaa gcgataacct gttgattatt gaatctttcg gggagatggc ttataacatt
     1321 tcttacctga ccagggtacc gggaaccaac accttactgg cgtgttgctg tcttttaaga
     1381 ccagaagagg ttaacagtga atattgaaga gttaaaaaaa caagccgaaa cggaaatcgc
     1441 cgactttatc gcgcaaaaaa tcgccgagct gaacaagaat acagggaaag aagtctctga
     1501 aattcgcttc accgcacgag aaaaaatgac cgggcttgaa agttatgatg tcaaaatcaa
     1561 aataatgtga ttttgtgaac atcaccccgt gcgaggtgat gttccgcttg ttgctaattt
     1621 agtgcccaat cattggcgat tgcggaatta agcgttggta caattcctcc ggcaccgggc
     1681 tttgccatac tcccgcatac attgcgtaac caatcaccgc aaacataatc cccagaacca
     1741 gtagcgtcat taaccagcca gacaacgcaa aggctttttt atttgccgca ggtttttgca
     1801 gtgaaaaggt caatgttgag gctaccggac atgactctac gcaagtcata cagccggtac
     1861 attccacgct tcgtacctga attaatttat cgaccgggat ccgtgatggg caactttttg
     1921 cgcatttgcc acagtcgata caactttcgg cattgcgacg aatcttaaac ggcgataata
     1981 gcgaaaccac gcccatcagc gcgccatatg ggcaaagata acgacaccag gcatggcgaa
     2041 taaacaggct ggcaatcagc aaaacggtca cgctgattaa tgtcgcggtc cccatatgac
     2101 gaaagaaatc gagcatttta acgtccatca ccacgctgta gggcgacaac ataaaatagt
     2161 gaatcgcctg agcgggcatc aataacgcaa tataaagaaa aaaactcaac agcaaatact
     2221 tcacgccgcg cagaggagta tccagccagc ggggaaggac aaattgccga ccaaacagtt
     2281 tgttaccgag atcgccgatt aattcagaaa gcgtaccaac cgggcataac catgaacaaa
     2341 aggccttttt taataataga ctgatgacga taaaagcgac caataacagc atcgcggcgg
     2401 cgtggacgga cggtaactgg cctgttgcaa ggctatattt cagattcatc agaccggcaa
     2461 tcggtagcca gccttcgata cctcccggtc tggtgacaaa tgtcgtacta cctgccgttt
     2521 cgtaatagcg cacccaatac cagaacgtga tggcaatata aatattcatt gccaacagta
     2581 ataattgcgt cgctttacgc caggtcgtgg cattgcgcca gtcattccac ggtaatttgc
     2641 cgcccgtcgt gcctggccgc cgctgccagc gggttcttct tttctctgtc atgattttgc
     2701 cagtccgtta agttgcatac caaatgccac tattctagtt gttcttaact ggctgatatt
     2761 gattcaaatc gcgttcaggt ctttcttatg caaccatgct tccagagcgg caacactgcg
     2821 tgtaatttct tcgtgtggaa ggggggcaga taatggctgc tgctccagtt gtgcgcatag
     2881 ctggctggcg ctacgcattc ccagactcga gcaactgctt tttagctgat gtgcggcaca
     2941 ctttattttc tcgttatcct gactggcgcg ggcaatgtcg atttcatcga gaagcggcag
     3001 ggcatgttgt gaaaataata ccagccattc gtggatcttc tccgtcccca ttaactgagc
     3061 atcttcattg agttgcgata catccagcgg ttgatcgtta ttgacttgca gttggagata
     3121 gtgcgccagt aactgaccga gcacttcacg cggcaccggt ttagggataa tcccgcgaaa
     3181 tagcgaactg gtacgctggc gcaacgtttc gtcgatgaca tgggcgctaa agccaatcaa
     3241 aaccagcgac ggatattgct gtgccagttg ccgggcaagc gtaatgccgt cgatatccgg
     3301 cagatcaaaa tccaccagtg cgacagcaaa tggttcacta ttttgcagtg tctctaaagc
     3361 ctgcgtggca tttcctacgg caacgacctg cgccccactg ctgttcaaca tctcaacggt
     3421 aatccgctgg gttagcgggt tatcttcaat taacagcaaa cgtaaaccgt ccagacgcac
     3481 cgcctgattg actgtttttg gcacgggtgc agtggcaata cgtaacagca agcgtaaaca
     3541 aaaacagctt ccaacctccg gcgtgctggt ggcgctcagt tcgccaccca tcgcctgggc
     3601 cagacggcta ctgatagtca gccccagccc ggtgccgccg cgtttgccgc taacctgaac
     3661 aaatggctgg aagatttcag ccagtttcgc gggatcaata ccgcagccgc tgtcttccac
     3721 ttcgaccagc cattgttcgc catcagtgcg actacgcagg ataatgtgcc cttcgtcggt
     3781 gaaacgcagg gcgttgctca acaggttggt aatgacctga cgaatacgcc gcggatcgcc
     3841 cattaacgca gtcggtacat cgtcggcaat tgccgttgcc aggcgaatcg ggcgaccttt
     3901 cacccgtccg ctcattaatt gcagggtact ttccagcaac gggcgcggtt caaagggttc
     3961 atcgctgacc gaaacattct tgccgcctgc ttcgatagcc gaataatcga gaatatcgtt
     4021 gaggatggtc agcaacgatt cgccagagtc ggtaattgcc cgcaaatcat cacgctgagc
     4081 gttaagtgcg gggttatctg ccagtagctg tgcggtaccg agaataccgt acagcggtgt
     4141 gcggatctca tggctcatcg ccgccagaaa cgctgatttt gcctggttgg ctttttctgc
     4201 ttccgcccgt gcctgtcggt gttctatcac cagttcctgc aattcagctg tacgcgcttt
     4261 gacctgcgcc gccaactgtt cacggtggcg attcagtgca tgaacactgc tgcgaaacgc
     4321 atccatcagc cgcccgatgg tatccagctc ccgtacgcca gcggtttccg ggaaagggga
     4381 gtcaatatca ccgtccagca gtcgttgcag tgcctgtgtt tgttcggcaa gtggacgcgt
     4441 gactgagcga taaaccacgc gccagaggat cagtatcagt gcgcaaagtg aaaccatccc
     4501 cagcaataac aggctgtatt gcccgcgtgc actggctttt tccagatgcg ccagtccgtg
     4561 ctgattacgc agctcaatgg tgtcgaccag ctgactgact tcgctactaa actgcgcgaa
     4621 ttgggcgatg ttattttggg cgagagtttg caggcgattg ctgatttcat tgtcctgctg
     4681 atacagcgcc agcaaatcgt tatattggct aacggtagtt aacgttgttg cgacctgcga
     4741 acgaactccc ggatcttcaa tgcgtatttg ccgacgttgc agaattttca ccgcattatt
     4801 gagctgcttt tccagcgttg gcgcattttt ctggatctgt tccaacccca gattcatcac
     4861 catttgctgc acccgcaggg cgctaaggcg cagttcattc atctggttaa catactcaag
     4921 atcgatatca atcagccgat cgagtgcact ttctgcggcc tgacgctgat gttgttcgat
     4981 caaatcgtaa atcccggcct gggtcgctcc ggcggaggtt gccgcattat tcgcctgacc
     5041 ttgcgccagg cgtgcgattt catcagcggc ggcgactatc tgctgactga gttgctgttg
     5101 ttgctggcgc agttgcaaac gctgccccac cagctctcct tgttgacgta acgaacggga
     5161 aatctcctgc tcctgttgtt caatagcggt ggtatcaaaa ccttgttccc gtaacgcttg
     5221 cagcaacgca ttaatcttca ggctttgtgc ggtgagcatt cgcccctgcg cctgccacat
     5281 cttttcgtta tcggcactgg tcagattctg cgcggcgaaa agttcccagg cgctggcttc
     5341 gctcaactgg cgcgccatat tcatggtagg aatcaatgcc tgagtgttgt ctttttccac
     5401 ctggctgata aagcgcaggt tgtaccatcc caccagggta ctggtcaggg ttaacagcgc
     5461 catcagggca aagcccatcc agagtcttcg ggtcagggtt aaattcacgg tcggtgcact
     5521 ttaggtgaaa aaggttgagt cgcaaagcag aatgcatcta gcataaagcc ttattattga
     5581 tgaggctatc atgcgcgtac tgctattttt acttctttcc cttttcatgt tatcggcatt
     5641 ttcggctgat aacctgttgc gctggcatga tgcgcagcat tacacggtgc aagcctctac
     5701 gccgcttaaa gccaaacgcg catggaaact gtgcgcgctt tatcccagcc tgaaagattc
     5761 atactggtta tcgttgaact atggtatgca ggaggctgct cgccgctacg gtgtggattt
     5821 aaaagtgctg gaggcaggcg gctacagcca gttggctacc cagcaagcac aaatcgacca
     5881 gtgtaaacag tggggcgcag aggccatttt gctcggtagt agcacgacgt catttcccga
     5941 tctgcaaaag caggtagcaa atctgccggt gatcgaactg gtaaatgcta ttgatgctcc
     6001 ccacgtgaaa agccgcgttg gtgtgccctg gtttcagatg ggctatcaac cagggcgata
     6061 tcttgtgcaa tgggcgcacg gtaaaccact gaacgtgctg ttgatgcccg gccccgataa
     6121 cgccgggggt agtaaggaga tggtcgaggg ttttcgcgca gccattgccg gaagcccggt
     6181 gcgtattgtc gatattgcgc tcggtgacaa cgatattgaa atccagcgta acctgttgca
     6241 ggagatgctg gagcgccatc cagaaatcga cgtcgttgcc ggaacggcca ttgcggcaga
     6301 ggcggcaatg ggggaagggc gtaacctgaa aacgccgctt accgtggtat cgttttatct
     6361 gtcgcatcag gtataccgag gcctgaagcg agggagagtc attatggctg ccagcgatca
     6421 aatggtctgg cagggggaac tggcggttga gcaggccatc aggcaattac aggggcaatc
     6481 ggtgtctgat aatgtcagcc caccgatttt agtcatgacg ccgaaaaatg ccgaccgtga
     6541 acatatccgc cgctcgctgt caccaggggg atttcgtccg gtctattttt atcagcacac
     6601 atcagcggct aagaaataac cttcgccatg ttgcgtcacc agtaaatccg cgctgagttt
     6661 atggcgtaaa cgacgaatta acacatcgac ggtgcgcaag tcagggtttt ccacccgacg
     6721 cgcagagagc atacgcagca gacgttcacg gctaagaatt tcgcccggat tcgtcacaaa
     6781 tgccaccaac atttcatact ctgcgcgggt cagtttaatc ggctcgccat cccgctccag
     6841 cgtatggcgc gacacattca ggcaataacc ggcaaagcga tagcagttgt cctgtgtgtg
     6901 cggttgagct tgtcgcgcga ggtcgattcg ccagagcaga tttttcaccc gtactaccag
     6961 ttcgcgcagt tccagcggtt tggtgacgta atcgtctgcg cccatttcca gcccaacaat
     7021 acggtcaatc cgatcgctgc gtcccgtaac cagaataatc cccaccgttg agcgttctcg
     7081 cagggcgcgg gttaacatca ggccattttc atcgggcaaa ttaatatcca gcagaattaa
     7141 atccaccggc tgattctgca taatttcccg cagcccggca ccgctcgccg taacggaaac
     7201 ggtatacccc tcctgagtga agtaggattg taatcgcgcc tgggtaaccg gctcatcttc
     7261 aacaataaca atgtgatgtg gcatcagagg gttttactca ttctgttcat atctgttcat
     7321 attctgccgt aagccgttca tcctgaccag tgccgctgtt catatttact cattaagatc
     7381 gcttcactaa accataattt tacaggggct attatgcgga aactctggaa cgcgctacgc
     7441 cgacccagtg ctcgttggtc ggtactggcg ctggtcgcta ttgggattgt gattggcatt
     7501 gcgctgattg tattgccaca cgttgggatc aaagtcacca gcacaaccga attttgtgtc
     7561 agttgccaca gtatgcaacc ggtgtatgaa gaatataaac agtcggtgca cttccagaac
     7621 gcctccggcg tgcgagctga atgccatgac tgtcatatcc cgccggatat tccaggcatg
     7681 gtgaagcgca aactggaagc gagcaacgac atttaccaga cctttattgc ccactccatt
     7741 gatacacctg aaaaattcga agccaaacgc gcggaacttg ccgagcgtga atgggcgcga
     7801 atgaaagaaa acaactcggc aacctgtcgc tcctgccata actacgatgc gatggatcat
     7861 gccaagcagc atcctgaagc ggcgcgtcag atgaaggtgg cagcgaaaga taatcaatcc
     7921 tgcatcgact gtcataaagg tattgcccac cagttaccgg atatgagtag tggcttccgt
     7981 aagcagttcg atgagctgcg cgccagtgct aatgatagtg gtgacacgct gtactccatt
     8041 gatattaagc cgatttatgc ggcgaaaggc gataaagaag cgtctggttc tctgctgcct
     8101 gcttcggaag tgaaagtcct taaacgtgac ggcgactggc tgcaaattga aatcactggt
     8161 tggacggaaa gtgcaggacg tcagcgtgta ctcacccaat tcccaggtaa acgcatcttt
     8221 gttgcctcga ttcgtggtga tgtgcagcag caggtgaaaa cgctggagaa aaccaccgtt
     8281 gccgacacca ataccgagtg gagcaagttg caggctactg cgtggatgaa gaaaggcgac
     8341 atggtgaacg atatcaaacc gatctgggct tatgcggatt cgctgtacaa cggcacctgt
     8401 aaccagtgcc acggcgcacc ggaaatatcc cactttgacg ctaacggttg gatcggcacg
     8461 ctcaacggca tgattggctt taccagcctc gataaacgtg aagaacgcac cttgttgaaa
     8521 tatctgcaaa tgaatgcgtc tgacaccgca ggtaaagctc acggcgataa gaaggaagaa
     8581 aaataatgaa caataacgat ctctttcagg catcacgtcg gcgttttctg gcacaactcg
     8641 gcggcttaac cgtcgccggg atgctggggc cgtcattgtt aacgccgcgc cgtgcgactg
     8701 cggcgcaagc ggcgactgag gctgtcatct cgaaagaggg cattcttacc gggtcgcact
     8761 ggggggctat ccgcgcgacg gtgaaggagg gtcgctttgt ggcagcaaaa ccgttcgaac
     8821 tggataaata tccgtcgaaa atgatcgccg gattgccgga tcatgtacac aacgcggcgc
     8881 gtattcgtta cccgatggta cgcgtggact ggctgcgtaa gcgccatctg agcgacacct
     8941 cccagcgcgg tgataaccgt tttgtgcgcg tgagctggga tgaagccctc gacatgttct
     9001 atgaagaact ggaacgcgta cagaaaattc acgggccgag tgccttgctg accgccagtg
     9061 gttggcaatc gacggggatg ttccataacg cttcggggat gctggcgaaa gcgattgcct
     9121 tgcatggtaa tagcgtgggt acaggcggag attactctac cggggctgcg caggtgatcc
     9181 tgccgcgcgt agtcggttcg atggaagtgt atgaacagca aacctcctgg ccgctggtat
     9241 tgcagaacag caaaaccatt gtgctgtggg gctccgattt gctgaaaaac cagcaagcga
     9301 actggtggtg cccggatcac gatgtttatg aatattacga gcagctaaaa gcgaaagtcg
     9361 ccgccggtga aattgaggtc atcagcatcg atccggttgt cacatccacc catgagtatc
     9421 tggggcgcga gcatgtgaag cacattgcgg ttaacccgca aactgacgtg ccgctgcaac
     9481 tggctctggc gcatacgctg tacagtgaaa acctgtacga caaaaacttc ctcgctaact
     9541 actgtgtagg ttttgagcag ttcctgccgt atctgctggg tgagaaagac ggtcagccga
     9601 aagatgccgc atgggctgaa aaactgacag gtattaatgc cgaaaccatt cgtgggctgg
     9661 cgcggcagat ggcggcgaac agaacgcaga ttattgctgg ctggtgcgtg cagcgtatgc
     9721 agcacggtga acagtgggcg tggatgattg tcgttctggc ggcgatgttg gggcaaattg
     9781 gcctgccagg tggaggtttt ggttttggct ggcactataa cggcgcaggc acgccggggc
     9841 gtaaaggcgt tattctgagt ggtttctccg gctctacgtc gattccgcct gttcacgaca
     9901 acagcgacta caaaggctac agcagcacca ttccgattgc ccgttttatc gatgcgatcc
     9961 tcgaaccggg gaaagtgatc aactggaacg gtaaatcggt aaaactaccg ccgctgaaaa
    10021 tgtgtatttt tgccggaact aacccgttcc atcgccatca gcagatcaac cgcattattg
    10081 aaggctggcg caagctggaa acggttatcg ccatagataa ccagtggacc tcaacctgcc
    10141 gctttgccga tatcgtgctg cctgcgacca cgcagtttga gcgtaacgat ctcgaccagt
    10201 acggcaacca ctccaatcgt ggcattatcg ccatgaaaca ggtcgtgccg ccgcagttcg
    10261 aggcgcgcaa cgacttcgat attttccgcg agctgtgccg tcgctttaat cgcgaagaag
    10321 cctttaccga agggctggac gaaatgggct ggctgaaacg catctggcag gaaggtgtac
    10381 aacagggcaa aggacgcggc gttcatctgc cagcgtttga tgacttctgg aataataaag
    10441 agtacgtcga gtttgaccat ccgcagatgt ttgttcgcca ccaggcattc cgcgaagatc
    10501 cggatctcga accgctgggc acgccgagtg gcctgattga gatctactcg aaaaccatcg
    10561 ctgatatgaa ctacgacgat tgtcaggggc atccgatgtg gtttgagaaa atcgaacgct
    10621 cccacggtgg gcccggctcg cagaagtatc cgttgcatct gcaatctgtg catccggatt
    10681 tccgacttca ctcgcagtta tgtgagtcgg aaacgctgcg tcagcaatat acggtagcgg
    10741 gcaaagagcc agtgttcatt aacccgcagg atgccagcgc gcgcggtatt cgtaacggtg
    10801 atgtggtacg cgtgtttaac gctcgcggtc aggtgttggc aggggcagtg gtttctgacc
    10861 gctatgcacc cggcgtggcg cgaattcatg aaggggcatg gtacgatcca gataaaggcg
    10921 gcgagcctgg tgcgctgtgc aaatacggta accccaacgt gttgaccatc gacatcggta
    10981 cttcgcagtt ggcgcaggcg accagtgcgc ataccacgct ggtggaaatt gagaagtaca
    11041 acggaacagt ggagcaggtg acggcgttta acggccctgt tgagatggtg gcgcagtgcg
    11101 aatatgttcc cacgtcgcag gtgaaatcat gaccacgctg acagcacaac agattgcctg
    11161 tgtttacgcc tggctggcgc agttgttctc ccgtgaactg gacgatgaac aactgacgca
    11221 aatcgccagt gcgcagatgg ctgaatggtt ttcgttgctg aaaagcgaac ctccgctcac
    11281 tgcggcggtg aatgggctgg aaaacagtat tgccactctg acagtacgtg acgatgcccg
    11341 tctggaactg gccgctgact tttgcggcct gtttctgatg accgacaaac aagcggcgct
    11401 gccgtatgca tcggcctaca aacaggacga gcaagagatt aaacgcttgt tagttgaggc
    11461 ggggatggaa accagcggca atttcaacga accggcagat catctggcga tctatctcga
    11521 tttgttgagt catctgcatt tttcgctggg agaggggacc gttcctgcgc gaagaatcga
    11581 cggtttgcgg caaaaaacac tgacggctct gcgggaatgg ttaccagagt ttgctgcgcg
    11641 ttgtcgacag tatgacagct ttggttttta cgcggcacta agccagttat tgctggtgtt
    11701 agtggagtgt gactaccaaa aaagataatg ccgtttgtag gcctgataag acgcggcagg
    11761 cgtcgcatca ggcattag
//
LOCUS       DAPHCV010000128        11558 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.129, whole genome shotgun sequence.
ACCESSION   DAPHCV010000128 DAPHCV010000000
VERSION     DAPHCV010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.129"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            49..918
                     /locus_tag="RH033_003914"
     CDS             49..918
                     /locus_tag="RH033_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HDV2993029.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSAMELR
                     DLLIALESRLYHKHKLDAEARLQYEQARDKAMKKMAENIPEIQIEELKNADINRRVNM
                     LELTDNQGENLSFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFNHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(968..1321)
                     /locus_tag="RH033_003915"
     CDS             complement(968..1321)
                     /locus_tag="RH033_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HDV2993030.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1459..3105)
                     /gene="groL"
                     /locus_tag="RH033_003916"
     CDS             complement(1459..3105)
                     /gene="groL"
                     /locus_tag="RH033_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDV2993031.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3149..3442)
                     /locus_tag="RH033_003917"
     CDS             complement(3149..3442)
                     /locus_tag="RH033_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDV2993032.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3718..4974
                     /gene="yjeH"
                     /locus_tag="RH033_003918"
     CDS             3718..4974
                     /gene="yjeH"
                     /locus_tag="RH033_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HDV2993033.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWNSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVLLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALGINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(4990..5466)
                     /gene="fxsA"
                     /locus_tag="RH033_003919"
     CDS             complement(4990..5466)
                     /gene="fxsA"
                     /locus_tag="RH033_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HDV2993034.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDHDRLDHKDDRQD"
     gene            5803..7239
                     /gene="aspA"
                     /locus_tag="RH033_003920"
     CDS             5803..7239
                     /gene="aspA"
                     /locus_tag="RH033_003920"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HDV2993035.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7357..8658
                     /gene="dcuA"
                     /locus_tag="RH033_003921"
     CDS             7357..8658
                     /gene="dcuA"
                     /locus_tag="RH033_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HDV2993036.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8773..9111
                     /gene="cutA"
                     /locus_tag="RH033_003922"
     CDS             8773..9111
                     /gene="cutA"
                     /locus_tag="RH033_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HDV2993037.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSLQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9087..10784
                     /gene="dsbD"
                     /locus_tag="RH033_003923"
     CDS             9087..10784
                     /gene="dsbD"
                     /locus_tag="RH033_003923"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HDV2993038.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNEASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTVNDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10821..11396
                     /locus_tag="RH033_003924"
     CDS             10821..11396
                     /locus_tag="RH033_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV2993039.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSHADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT         2767 a         2995 c         2862 g         2934 t
ORIGIN      
        1 taaattaaaa ttaattattt aattgagaaa tggttaggga gaacctacat ggccataagc
       61 atcaagggtg ttaatactgg tgttattcgc aagagtaata atttcattgc gctggcgctg
      121 aaaataaaag agcctcgcaa taaagaatcg ttgttcttca tgtctgcgat ggaactgcgc
      181 gaccttttga tagcactgga aagccgtctg tatcacaagc ataaactcga cgctgaggct
      241 cgtcttcagt atgagcaggc gcgcgataaa gccatgaaaa agatggcaga aaacattcca
      301 gaaatacaga ttgaagagct taaaaatgcc gatattaatc gccgagttaa tatgctggaa
      361 cttaccgata atcagggcga aaatcttagc ttcgtattaa cgttacatga cggtagcaaa
      421 tgtgagttag tcgtcaatga gttgcagatt gaaatgctgg cgcgagcaat cattcatgca
      481 attaataatg cggaaatgcg tgaactggca ttacgtatta cctcactgtt agatttcctg
      541 ccgttatacg acgttgactg ccaggacaat ggcaatctgg aatatgacac ctattcccaa
      601 ccggaatgga aacataactt gttcaaccat tatctggcgg tgctttaccg ctttaaagat
      661 gaaagtggca aagaacaatt tagcggcgca gtggtgaaaa cacgtgaagc aacgccgggt
      721 aaagaagtgg aggcgatcac tcgccgaatg cttgatttca gcccacgact gaaaaaactt
      781 gcgggtgtac catgtcaggt ctatgttcgc accgtagcgg caaataacgc ccaaccgcta
      841 acccaggatc aatgcttacg cgcgctgcat cacttacgtg ttcaatccac cagcaaaact
      901 gcaccacagg cgaaataatc ccatctgttt tactacgagg ctggaagtat ccagcctctg
      961 gatcacatca gttcgggcac ttatatacct ggccgataat ctggctatcc gtcgggacaa
     1021 aactggacaa cattccctgc gacgggctgc tgatgccata aatcacgtta ccgcccattg
     1081 cagccgcctg gttgcgcaga tcatttgctg cgccgcgcat agaaccgccc tcttcaccgt
     1141 gttgcccgga aagccagttg ctttgcttac ctgtcgcagt accaatcagc tggcactctg
     1201 cgcctggctg ctcgtccaca atgcgtacac tctgaccggc agcactcaat tcgttgctgg
     1261 agctacaacc cgccatcagt agcgcggcac cgacaatccc tgctaagtat tttacgtgca
     1321 tgttattccc cataatcaat gagctggacg ccactcgcgt cgtccgtgtc tgaatcttat
     1381 actagaaaga tgaccaaaag aaaaaccccc agacatttct gcccgggggt ttgtttattt
     1441 ctgcgaggtg cagggcaatt acatcatgcc gcccatgcca cccatgccgc ccataccgcc
     1501 agcagcgcct aagtcagctg catcattttt cggcaggtcg gtaaccatgc actcggtggt
     1561 gatcatcagg ccagccacag aagccgcgta ctgcagagca gaacgggtta ctttggttgg
     1621 atccaggata cccatgtcga tcatgttgcc gtattcttcg gttgctgcgt tgtaaccata
     1681 gttgccgtcg ccgcctttaa cagtgttagc aacaacagac ggttcttcgc cgcagttcag
     1741 gacgatctga cgcagcggag cctccattgc acgcagtgca actttgatac ccacgttctg
     1801 gtcttcgttc tgaccacgca ggtcagccag tttagacgct acgcggatca gcgcaacacc
     1861 accaccagca accacgcctt cttctaccgc agcacgggtc gcgtgcaggg cgtcttcaac
     1921 gcgtgctttt ttctctttca tttcaacttc ggtagcagca cctactttga taactgcaac
     1981 gccgcctgcc agtttcgcta cgcgctcctg cagtttttca cggtcgtagt cagaagttgc
     2041 ttcttcaatc tgctgacgga tctgagcaac acggccctgg attgcagctt cttcacccac
     2101 gccatcgatg atggtggtgg tgtctttgtt gatcacaaca cgtttagcct gacccaggtc
     2161 ttccagggtt gctttttcca gctccatacc gatctcttca gagattacgg taccgccagt
     2221 cagggttgcg atatcctgca gcatagcttt acgacgatcg ccgaagcccg gagctttaac
     2281 cgcagcaact ttcacgatgc cacgcatggt gttaacaacc agagttgcca gtgcttcgcc
     2341 ttcaacatct tcagcgatga tcagcagcgg tttacctgct ttggcaacag cttccagaac
     2401 cggcagcatt tcgcggatgt tggagatttt cttgtcagcc aacaggatga acgggctttc
     2461 cagttctact gcgccagttt ccggcttgtt gatgaagtaa ggagacaggt agccacggtc
     2521 gaactgcata ccttcaacca cgtccagttc gtcctgcaga ccggtaccgt cttcaacggt
     2581 gataacgcct tctttaccga ctttgtccat cgcttcagcg atcagtttac ctacggtttc
     2641 gtcggagtta gcagagatgg taccaacctg agcaatcgct ttagagtcag agcacggtac
     2701 ggacagcgct ttcagttctt caactgcagc ggtaacagct ttgtcgatac cacgtttcag
     2761 gtccatcggg ttcatgcccg cagcaacagc tttcagacct tcagtgatga tagcctgagc
     2821 cagtacggtt gcagtggtgg taccgtcgcc tgcagcgtcg ttcgctttag aggcaacttc
     2881 tttcaccatc tgcgcaccca tattttcgaa cttgtcttcc agttcgattt cacgagcaac
     2941 ggaaacacca tctttggtga tggtcggtgc accgaaagat ttatccagaa ctacgttacg
     3001 gcctttcgga ccgagggtaa ctttcactgc atctgccagt acgtttacgc cgcgcagcat
     3061 tttcacacga gcgtcgttac cgaattttac gtctttagct gccattatct ttattcctta
     3121 aattcgtatg ttcagtgtcg tgcgcggatt acgcttcaac aattgccaga atgtcgcttt
     3181 cggacatgat caacacttct tcattgtcga tcttctcaga tttcacaccg tagccatcgt
     3241 tgaaaataac gatgtcgcca actttcacat ccagcggctt cacttcgcca ttttcaagga
     3301 tacggccatt gccgacagcc agcacttcgc cgcgggtgga tttagccgct gcagagccgg
     3361 tcagaacgat gccgccagca gatttagttt caacttcttt acgcttgacg atcacgcgat
     3421 catgcaatgg acgaatattc attgataact ctcctttgag aaagtccgta tctgttatgg
     3481 gtgacgccgg agcttacgtg gtttcccggc tggtgaccag agaaatgggg atgaggcttc
     3541 gccccttcaa gggggaaaaa gaaaaaaaat tctgatcagc acaaaatcgg gtgaaaaccc
     3601 tgattcacct cacatttcat cgctatttct tcatcaccgt cataagcgaa tctgattgtg
     3661 ctaccatcga aaatctacgc agttgcccaa aatttgggcg caatcggaca tcaacccatg
     3721 agtggactca aacaagaact ggggctggcc cagggcatcg gcctgctatc gacgtcatta
     3781 ttaggcaccg gcgtgtttgc cgttcctgcg ttagctgcac tggtagcagg caataacagc
     3841 ctgtgggcgt ggcccgtttt gattatctta gtgttcccga ttgcgattgt gtttgcgatt
     3901 ctgggtcgcc actatcccag cgcaggcggt gtcgcgcact tcgtcggtat ggcgtttggt
     3961 tcacggcttg agcgagtcac cggctggctg tttttatcgg tcattcccgt gggtttgcct
     4021 gccgcactac aaattgcagc cggattcggc caggcaatgt ttggctggaa tagctggcaa
     4081 ctgttgttgg cagaactcgg tacgctggcg ttggtgtggt atatcggtac tcgcggtgcc
     4141 agttccagtg ctaatctaca aaccgttatt gccggactta tcgtcgcgct gattgtcgct
     4201 atctggtggg cgggcgatat caaacctgcg aatatcccct tccctgcgcc aggaaatatc
     4261 gaacttaccg ggttatttgc tgcgttatca gtgatgttct ggtgttttgt cggtctggaa
     4321 gcatttgccc atcttgcctc ggaatttaaa aatccagagc gtgattttcc tcgtgctttg
     4381 atgattggtc tgctgctggc aggattagtc tactggggct gtacggtagt cgtcttacac
     4441 ttcgacgcct atggtgaaca aatggccgcg gcagcatcgc ttccaaaaat tgtagtgctg
     4501 ttgttcggtg taggagcgtt atggattgcc tgcgtgattg gctatctggc ctgctttgcc
     4561 agtctcaaca tttatataca gagcttcgcc cgcctggtct ggtcgcaggc gcaacataat
     4621 cctgaccact acctggcacg cctctcttct cgccatatcc cgaataatgc cctcaatgcg
     4681 gtgctcggct gctgcgtggt gagcacgttg gtgattcatg ctttagggat caatctggac
     4741 gcacttatca tctatgccaa tggcatcttt attatgattt atctgttatg catgctggca
     4801 ggctgtaaat tattgcaagg acgttatcga ctactggcgg tggttggcgg gctgttatgc
     4861 gttctgttac tggcaatggt cggctggaaa agtctctatg cgctaatcat gctggcgggg
     4921 ttatggctgt ttctgccaaa acgaaaaacg ccggaaaatg gcataaccac ataatccggc
     4981 gtttcggcat taatcctggc gatcgtcttt atgatcaagg cggtcgtggt catcatcctt
     5041 tcgctggtac tcaccatcaa aagtattacc gccaccggtc ccggcgctaa aaccgccgcc
     5101 tggcatgcga gaaaagcgca aatgcggcat caacttcact gtcagatgct tttgcaccgg
     5161 cggcaataaa agcagaagac cgaggaagtc ggtgaaaaag cccggtaata aaagcagcaa
     5221 accggcaatg atcagcgaaa cacttttaat catctccgcc gctgggtttt cacccgccgc
     5281 cattttttgc tgcatcagca cgaaattctt aaagccctgg ttgcgtacca gtgacatacc
     5341 gataactgac gtgaatataa ccagcacgag ggtcagcaat acccccaata catgggcaac
     5401 ctgaataaag attgaaatct caatatagac ataaaggaaa atggcaataa aaggtaacca
     5461 gcgcaaaggt ttctcctgta atagcagccg gttaaccccg gctacctgaa tgggttgcga
     5521 atcgcgttca gcttatattg tggtcattag caaaatttca agatgtttgc gcaactattt
     5581 ttggtagtaa tcccaaagcg gtgatctatt tcacaaatta ataattaagg ggtaaaaacc
     5641 ggcacttaaa gtgatccaga ttacggtaga aatcctcaag cagcatatga tctcgggtat
     5701 tcggtcgatg caggggataa tcgtcggtcg aaaaacattc gaaaccacat atattctgtg
     5761 tgtttaaagc aaatcattgg cagcttgaaa aagaaggttc acatgtcaaa caacattcgt
     5821 atcgaagaag atctgttggg taccagggaa gttccagctg atgcctacta tggtgttcac
     5881 actctgagag cgattgaaaa cttctatatc agcaacaaca aaatcagtga tattcctgaa
     5941 tttgttcgcg gtatggtaat ggttaaaaaa gccgcagcta tggcaaacaa agagctgcaa
     6001 accattccta aaagtgtagc gaatgccatc attgccgcat gtgatgaagt cctgaacaac
     6061 ggaaaatgca tggatcagtt cccggtagac gtctaccagg gcggcgcagg tacttccgta
     6121 aacatgaaca ccaacgaagt gctggccaat atcggtctgg aactgatggg tcaccagaaa
     6181 ggtgaatatc agtacctgaa cccgaacgac catgttaaca aatgtcagtc cactaacgac
     6241 gcctacccga ccggtttccg tatcgcagtt tactcttccc tgattaagct ggtagatgcg
     6301 attaaccaac tgcgtgaagg ctttgaacgt aaagctgtcg aattccagga catcctgaaa
     6361 atgggtcgta cccagctgca ggacgcagta ccgatgaccc tcggtcagga attccgcgct
     6421 ttcagcatcc tgctgaaaga agaagtgaaa aacatccaac gtaccgctga actgctgctg
     6481 gaagttaacc ttggcgcaac agcaatcggt actggtctga acacgccgaa agagtactct
     6541 ccgctggcag tgaaaaaact ggctgaagtc actggcttcc catgcgtacc ggctgaagac
     6601 ctgatcgaag cgacctctga ctgcggcgct tatgttatgg ttcacggcgc gctgaaacgc
     6661 ctggctgtga agatgtccaa aatctgtaac gacctgcgct tgctctcttc tggtccacgt
     6721 gccggcctga acgagatcaa cctgccggaa ctgcaggcgg gctcttccat catgccagct
     6781 aaagtaaacc cggttgtccc ggaagtggtt aaccaggtat gcttcaaagt catcggtaac
     6841 gacaccactg ttaccatggc agcagaagca ggtcagctgc agttgaacgt tatggagccg
     6901 gtcattggcc aggccatgtt cgaatccgtt cacattctga ccaacgcttg ctacaacctg
     6961 ctggaaaaat gcattaacgg catcactgct aacaaagaag tgtgcgaagg ttacgtttac
     7021 aactctatcg gtatcgttac ttacctgaac ccgttcatcg gtcaccacaa cggtgacatc
     7081 gtgggtaaaa tctgtgccga aaccggtaag agtgtacgtg aagtcgttct ggaacgcggc
     7141 ctgttgactg aagcggaact tgacgatatt ttctccgtac agaatctgat gcacccggct
     7201 tacaaagcaa aacgctatac tgatgaaagc gaacagtaat cgtacagggt agtacaaata
     7261 aagaaggcac gtcagatgac gtgccttttt tcttgtgagc agtaacttaa aaataacaat
     7321 ctaatatcaa cttgttaaaa aacaaggaag gctaatatgc tagttgtaga actcatcata
     7381 gttttgctgg cgatcttctt gggcgccaga ttggggggaa taggtattgg ttttgcaggc
     7441 ggattggggg tgctggttct tgccgctatt ggcgttaaac ccggtaacat cccgttcgat
     7501 gtcatttcca ttatcatggc ggttatcgcc gctatttctg ccatgcaggt tgctggcggt
     7561 ctggactatc tggttcatca gacagaaaag ctgctgcgcc gtaacccgaa atacatcacg
     7621 atcctcgcac cgatcgtgac ctatttcctg actatctttg ctggtactgg caacatctct
     7681 ctggcgacac tgccagttat cgctgaagtt gcgaaggaac aaggcgttaa accttgccgt
     7741 ccgctgtcta ctgcagtggt atccgcgcag attgcgatca ccgcatcgcc aatctcagca
     7801 gcagtggttt acatgtcttc cgtgatggaa ggtcatggca tcagctacct ccatctgctc
     7861 tccgtggtca tcccgtccac cctgctggcg gttctggtga tgtccttcct ggtcactatg
     7921 ctgttcaact ccaaactctc tgacgatccg atttatcgca agcgtctgga agagggcctg
     7981 gttgaactgc gcggtgaaaa gcagattgaa atcaaatccg gtgcaaaaac gtccgtctgg
     8041 ctattcctgc tgggcgtagt tggcgtggtt atctatgcaa tcatcaacag cccaagcatg
     8101 ggtctggttg aaaaaccgct gatgaacacc accaacgcaa tcctgatcat catgctcagc
     8161 gttgcaactc tgaccaccgt tatctgtaaa gtcgataccg acaacatcct caactccagc
     8221 accttcaaag caggtatgag cgcctgtatt tgtatcctgg gtgttgcgtg gctgggcgat
     8281 actttcgttt ccaacaacat cgactggatc aaagataccg ctggtgaagt gattcagggt
     8341 catccgtggc tgctggccgt catcttcttc tttgcttctg ctctgctgta ctctcaggct
     8401 gcaaccgcaa aagcactgat gccgatggct ctggcactga acgtttctcc tctgaccgct
     8461 gttgcttctt tcgctgcggt gtctggtctg ttcattctgc cgacctaccc gacgctggtt
     8521 gctgcggtac agatggatga cacgggtact acccgtatcg gtaaattcgt cttcaaccat
     8581 ccgttcttca tcccgggtac tctgggtgtt gccctggccg tttgcttcgg cttcgtgctg
     8641 ggtagcttca tgctgtaatg accaatcgcg gggcttcagg ccccgctttc tttcccgccg
     8701 actaacatcc ttcccccgtc cgttgtatag tgacctctct cttgcggttc catctgttct
     8761 tgcgaggtgt ttatgcttga tgaaaaaagt tcgaataccg cgtctgtcgt ggtgctatgt
     8821 acggcaccgg atgaagcgac agcccaggat ttagccgcca aagtgctggc ggaaaaactg
     8881 gcggcctgcg cgaccttgat ccccggcgct acctctctct attactggga aggtaagctg
     8941 gagcaagaat acgaagtgca gatgatttta aaaactaccg tatctctcca gcaggcactg
     9001 ctggaatgcc tgaagtctca tcatccatat caaaccccgg aacttctggt tttacctgtt
     9061 acacacggag acacagatta cctctcatgg ctcaacgcat ctttacgctg atcctgctac
     9121 tttgcagcac ttccgttttt gccggattat tcgacgcgcc gggacgttca caatttgtcc
     9181 ccgcggatca agcctttgct tttgattttc agcaaaatca acatgatctg aatctgacct
     9241 ggcagatcaa agacggttac tacctctacc gtaaacagat ccgcattacg ccggaacacg
     9301 cgaaaattgc tgacgtgcag ctgccgcaag gcgtctggca tgaagatgag ttttacggca
     9361 aaagcgagat ttaccgcgat cggctgacgc ttcccgtaac catcaaccag gcgagtgcgg
     9421 gagcaacgtt aactgtcacc taccagggct gtgctgatgc cggtttctgt tatccgccag
     9481 aaaccaaaac cgttccgtta agcgaagtgg tcgccaacaa cgaagcgtca cagcctgtgt
     9541 ctgttccgca gcaagagcag cccaccgcgc aattgccctt ttccgcgctc tgggcgttgt
     9601 tgatcggtat tggtatcgcc tttacgccat gcgtgctgcc aatgtaccca ctgatttctg
     9661 gcatcgtgct gggcggtaaa cagcggcttt ccactgccag agcattgttg ctgaccttta
     9721 tttatgtgca ggggatggcg ctgacttaca cggcgctggg tctggtggtt gccgccgcag
     9781 ggttacagtt ccaggcggcg ctacagcacc catacgtgct cattggcctc gccatcgtct
     9841 ttaccttgct ggcgatgtca atgtttggct tgtttactct gcaactcccc tcttcgctgc
     9901 aaacacgtct cacgctgatg agcaatcgcc aacagggcgg ctcacctggc ggtgtgttta
     9961 ttatgggggc gattgccgga ctgatctgtt caccatgcac cactgcaccg cttagcgcga
    10021 ttctgttgta tatcgcccaa agcgggaaca tgtggctggg gggcggcacg ctttatctct
    10081 atgcgttggg catgggcctg ccgctgatgc taataaccgt ctttggtaac cgcctgctgc
    10141 cgaaaagcgg cccgtggatg gaacaagtca aaaccgcgtt tggttttgtg atcctcgcac
    10201 tgccggtctt cctgctggag cgagtgattg gcgatatatg gggattacgc ttgtggtcgg
    10261 cgcttggtgt cgcattcttt ggctgggcct ttatcaccag cctacaggcc aaacgcggct
    10321 ggatgcgcgt agtgcaaata atcctgctgg cagcggcatt ggttagcgtg cgcccacttc
    10381 aggattgggc atttggtgcg acgcataccg cgcaaactca gacgcatctc aactttacac
    10441 aaatcaaaac ggtagatgag ttaaatcagg cgctcgttga agccaaaggc aaaccagtga
    10501 tgttagatct ctatgccgac tggtgcgtcg cctgtaaaga gtttgagaaa tacaccttca
    10561 gcgacccgca ggtgcaaaaa gcgttagcag acacggtctt acttcaggcc aacgtcactg
    10621 tcaacgacgc acaagatgtg gcgctgttaa agcatcttaa tgtccttggc ctaccgacaa
    10681 ttctcttttt tgacggacaa ggccaggagc atccacaagc acgcgtcacg ggctttatgg
    10741 atgctgaaac cttcagcgca catttgcgcg atcgccaacc gtgaacgaca cttgcagtgg
    10801 gaaagacgga ggaaaatagc gtgcaacgtg aagatgtact gggagaagcc ctgaaattat
    10861 tagaattaca agggattgcc aacaccacgc tggagatggt tgctgaacgt gtggattatc
    10921 cactggacga gctacgccgc ttctggccag acaaagaggc aatcctctac gatgcgctgc
    10981 gttatcttag ccaacagata gatgtctggc gtcgtcagct gatgctggac gaaacgcaaa
    11041 ccgccgaaca aaagttgctg gcacgttatc aggcgttatc ggagtgcgtt aaaaacaacc
    11101 gctatccggg ctgtctgttt atcgctgcct gtacgtttta tcccgatcct ggccacccta
    11161 ttcatcaact ggccgatcag caaaaaagcg cggcctacga tttcacccac gaactgttaa
    11221 ccacgctgga agttgacgat ccggcgatgg tagcaaagca gatggaactg gtgctggaag
    11281 gctgtttaag ccgaatgctg gtgaatcgta gccacgcgga tgtcgacacc gcacatcggc
    11341 tggcagaaga tatcctgcgc ttcgctcgct gccgccaggg tggcgcactg acctgacaga
    11401 aacacagaaa agaagcgatt tgccgcaatc ttaagcagtt gaatcgcttt tactgaaatt
    11461 aggttgacga gatgtgcaga ttacggttta atgcgccccg ttgcccggat agctcagtcg
    11521 gtagagcagg ggattgaaaa tccccgtgtc cttggttc
//
LOCUS       DAPHCV010000129        11517 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.130, whole genome shotgun sequence.
ACCESSION   DAPHCV010000129 DAPHCV010000000
VERSION     DAPHCV010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.130"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(279..1244)
                     /gene="alx"
                     /locus_tag="RH033_003925"
     CDS             complement(279..1244)
                     /gene="alx"
                     /locus_tag="RH033_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HDV2993040.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(1528..2514)
                     /locus_tag="RH033_003926"
     CDS             complement(1528..2514)
                     /locus_tag="RH033_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDV2993041.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTAYLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKNVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFAELVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(2593..3276)
                     /locus_tag="RH033_003927"
     CDS             complement(2593..3276)
                     /locus_tag="RH033_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HDV2993042.1"
                     /translation="MQPRLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNTSKQL
                     TWSDVSAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(3353..3856)
                     /locus_tag="RH033_003928"
     CDS             complement(3353..3856)
                     /locus_tag="RH033_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDV2993043.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            3941..5077
                     /gene="rlmG"
                     /locus_tag="RH033_003929"
     CDS             3941..5077
                     /gene="rlmG"
                     /locus_tag="RH033_003929"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HDV2993044.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            5268..5351
                     /locus_tag="RH033_003930"
                     /pseudo
     CDS             5268..5351
                     /locus_tag="RH033_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            5348..5764
                     /gene="higA"
                     /locus_tag="RH033_003931"
     CDS             5348..5764
                     /gene="higA"
                     /locus_tag="RH033_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HDV2993045.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(5809..7827)
                     /gene="fadH"
                     /locus_tag="RH033_003932"
     CDS             complement(5809..7827)
                     /gene="fadH"
                     /locus_tag="RH033_003932"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HDV2993046.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDANQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSPLQAPINRFIPHELTHEEILQLIDNFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPINGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGEPQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(8053..10404)
                     /gene="ygjK"
                     /locus_tag="RH033_003933"
     CDS             complement(8053..10404)
                     /gene="ygjK"
                     /locus_tag="RH033_003933"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDV2993047.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVLVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSATLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKTWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTENGEMLFTVKKGAKEETLSGLNNYTRVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(10421..11491)
                     /gene="ygjJ"
                     /locus_tag="RH033_003934"
     CDS             complement(10421..11491)
                     /gene="ygjJ"
                     /locus_tag="RH033_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HDV2993048.1"
                     /translation="MKLITAPCRALLALPFCYAFSVTADEARPAEHDDTKTPAMTSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVSGSES
                     GMMVSAMYEALKGIYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHAPSDQLFLMQYFYW"
BASE COUNT         2686 a         3152 c         2970 g         2709 t
ORIGIN      
        1 tgcaccccca ggctctggcg aacaaaatcg ccgctgcgcg gttccctcag cttatccctt
       61 gcggctaccg ggtcgggcgt aagggaacgt ccctgtaaga tgcgccctcg ccgcgcatcc
      121 atgcgcgtcg ccccggcctc cagggaacgc ttcggcgatt ttgacgccac caacacccgt
      181 gcggtctttt tgtaaagagc tataagccga ttacataatt aatagctcac ctttgtctgg
      241 aatgagttat ctctagtaat ggcttaatat acagcgaatc attctccccg ctgcttatca
      301 tgccgataat tcacccaggc gttgataata aacgtcatca ccagaatgcc aaacaccacg
      361 cccagcgaga cggcgatcgg aatatggtag aagtcgacaa tcagcatctt gataccgata
      421 aacaccagaa tcaccgccag accatatttg agcatcgaga aacgctctgc cacacctgcc
      481 agcaggaaat acatcgcacg caggccgagg atcgcaaaca ggtttgaggt cagcacaatg
      541 aacgggtcag tggtcacggc gaagatagcc ggaatgctat ccacggcgaa aatcacgtcg
      601 ctcaattcca ccagaatcag caccagcatc agcggcgtgg catacagcaa gccattctta
      661 cgcacaaaga aatgctcgtt gtcgatggtg tcggtcatgc gcaaatgacc gcgcagccag
      721 cgcaccagcg gcttgtcgcc aatgcctgat tcatcttcat gggccagcgc catcttcacg
      781 ccggtaaaca gcaggaaggc accgaagata tacaggatcc agtcgaactg cgaaatcaac
      841 cagctgccag tgaagatcat gatggtacgc agaacaatcg ccccgagtac gccatacacc
      901 agcacgcgcc gttgtaacgc cgccggaaca gagaaatagc tgaacaacat cagccagaca
      961 aagacgttat cgaccgccag cgatttctca atcagataac ccgtgagaaa ggccagtgcc
     1021 tgtggatcgg caacggcgcg accttcggtt tgcaccagat accaccagaa agcggcgtta
     1081 aacagtaacg acagcgtcac ccagaccagc gaccaggccg cagcctgttt catggtcatg
     1141 gcatgtgccc cacgacgccc ctgcaacaac aggtcgatag ccagcataat ggcgacaaca
     1201 acagcgaatc cgccccatag caacggcgtg ccgacagtat tcatagaagt tccttacaca
     1261 taaaaaaaac ggccaacgtc ggaagacgtc agccgctgct ttttatgcat agacctcgcc
     1321 ttccggcaag gtctcactta caacaaaaaa ggaatacgtc tgcgtattcc tttcgggttg
     1381 cccggtgacc ggatgtggtt tttcacacat cgtaatgacg atcgaccggc aatgaagtta
     1441 ctcccctttg caggtaacaa agtatgacag accattcagt cagtcaatgg tctgttacat
     1501 accttttaca ctgttttact ttggcaatta tagttttacg ctatctgccg gaaaaatcac
     1561 cccagtctgg cggcggatct cggtcagcag tttggcggtg atgcgactga ccgccagccc
     1621 aggatgatcc accagatgct catccaccag ttcagcaaac agctctgctt cgtagagcat
     1681 ggtattaata tgctgcggtt gggtgagatc ctgcatctgg ctgccacgtg gcacaaaaca
     1741 cactttctgg cattcagaca gtttttcaat caccagcgat cctgcttcgc cctgaatctc
     1801 gcttgccagg acagaatcgc tcacttttga gtgctgcaac gtaacgctga aatcaccgta
     1861 atccatcacc accacgccgt gggcatccac accgcttgcc agcaaactgg cggttgcctg
     1921 cacgtttttc ggttcaccga ataatgccac cgccgacgcc agacagtaaa agccgatatc
     1981 cataattgaa ccgttagaga atgctggatt aaaggtgttg ggattctcac catccaggta
     2041 acgttgatac cgtgaggaat actggcaata gttgaaaaag actttacgca gtttgccgac
     2101 tttcggcagt gcctggcgca acagatgaaa gttcggcagg taggccgttt taaatgcctc
     2161 aaacagcacc acctgatttt cccgcgcaca ggcaatggcg gcatccactt ccgccagatt
     2221 cgatgccagc ggcttctcgc aaatcacatg aattttgtga ctaaggaaaa gttgtgtctg
     2281 ggaaaagtgc agggcattcg ggctggcaat atacaccgcg tcaatggcat cgctttccgc
     2341 catcgcttcc agcgacgtaa acagatgctc gacagaaaaa tcattggcaa agtgctgggc
     2401 ctgttcaagg ctgcgggaat atacggcggt taacttgtat ttaccgctct catgggcggc
     2461 ctcgacgaac tggcgagtga tccagttcgt accaatcaca gcgaaacgta tcataacgcg
     2521 tggatactcc ataaagggat tctgtcgtca atgtagcacg cctttttcat cttcgacgtg
     2581 cgcagatacg cattattctt tcgccggaca gccatgctca ctaaacggcc caatcatgac
     2641 cgatggcccg agatatttcg gctggcgatg gaggattttt gcgtcaatca ccgcgctgac
     2701 tctggcgagt ttctcccgta actgagtata aaaaccgtgc ttcatattca ggtctggcgc
     2761 gttaaagccg atagcgtcga tgccgtactg ttttgccagc cagatggcgc gttggttatg
     2821 aaattcttgc gaaataatgg tgatatggtt ttccccaaac acctttttgg cacgcaccac
     2881 cgaatcaagc gttgagaatc cagcatagtc gcagaagatc actttcgccg gtacgccttt
     2941 agcgatcaaa gcctgctgca tacctgaggc ttcatcgtaa tttttacgcc cgttatcgcc
     3001 acttaccagt agccatttca cttttccggc gtggtacaac gctgctgcgg tatcaatacg
     3061 tcgggtaaag tagcgattac ccggcctcgc ccccagcaat aaacccacgt tacgcgccgg
     3121 aactgcgctg acatcgctcc aggtcagctg cttgctggtg ttgaccataa cgcgatcggc
     3181 aataaaaatg gtcgctcctg ctataagcaa caataaacag ccaattttaa gagtccggcg
     3241 agaaaaacaa atacggagaa gaaggcgggg ctgcatggtc gttatcctta acgaaagggg
     3301 ggaggtcgat tttaggcgct ataggaagaa aagataacgc ctgacatcat tttcagattt
     3361 tgtcctgccc aagcgacaac tgcgttagcc acagccgggt gtcgaactca agctggtggt
     3421 actgcggctc catatggcag cacaattgat agaacgcttt gttatgctct ttctccttca
     3481 ggtgcgccaa ctcgtgcacc acgatcatgc gcaaaaacgg ttccggcgcg ttgcgaaaca
     3541 cggtagcgac gcggatctcc gcctttgctt tcagcttgcc gccctgcaca cgcgaaacag
     3601 cggtatgtaa cccgagcgcg tttttcagca catggatctt attgtcatac atcactttag
     3661 tgatcggcgg ggcattgcgc agaaactgat ttttaagatc ctgagtatat tgccagaggg
     3721 ctttatcggt ggcgtagtcg tgtgttcccg gatagcgttt tgccagcaca tcgcccagac
     3781 gctgttcgtt aatcagcgtt cgcacctggg aaagtagctg ctcgggataa ccctgaagat
     3841 aagtaagatt gctcataacc gcccacgaaa aagagaaaaa gggtatactc acgcaccctt
     3901 ttcaggggca acgtcgaaat tttatcattc aggagggccg atgagccact tagacaacgg
     3961 tttccgttca ctgacactac aacgttttcc ggcgacggat gacgttaacc cactacaggc
     4021 gtgggaagcg gcggatgaat atttgctgca acagttggac gacacagaaa tccgcggccc
     4081 ggtgttgatc ctgaatgatg cctttggtgc gttaagttgt gcgctggcgg aacataagcc
     4141 atacagcatt ggcgactcat acatcagcga actggcgacg cgcgagaatt tacgcctcaa
     4201 cgggattgat gagtcgagcg tgaagtttct cgacagcacc gccgactacc cgcaacagcc
     4261 gggcgtggta ctgatcaaag taccgaaaac actggcgttg ctggaacaac aactgcgtgc
     4321 gctgcgcaaa gtggtcacgc cggatacacg tattattgcc ggtgccaaag cccgtgacat
     4381 ccacacttcc acgctggaac tgttcgaaaa agtgctcggc ccgaccacca ccacgctggc
     4441 atggaagaag gcacgcttaa ttaactgtac tttcaacgag ccgccgttgg ccgatgcgcc
     4501 gcagaccgtt agctggaaac tggaaggtac tgactggact atccacaacc atgcgaatgt
     4561 cttctcccgc accgggctgg atattggcgc gcgcttcttt atgcagcatc tgccagagaa
     4621 tctcgaaggt gagattgtcg atctcggttg tggtaatggc gttattggtc tgacgctgct
     4681 tgataaaaac ccgcaggcga aagtggtgtt tgtcgatgaa tcgccgatgg cggttgcttc
     4741 cagccgttta aacgttgaaa ccaacatgcc agaggcgttg gatcgctgcg agtttatgat
     4801 caacaacgcg ctctccggcg tggagccttt ccgttttaat gctgtgctct gcaacccgcc
     4861 gtttcatcag caacatgcgc tgaccgataa cgtcgcctgg gagatgttcc accacgcccg
     4921 ccgctgtctg aaaatcaacg gcgagctgta tatcgttgcc aaccgtcacc tggattactt
     4981 ccataaactg aagaagattt tcggcaactg caccaccatc gccacgaata ataaatttgt
     5041 ggtgctgaaa gcagtgaagc tggggcgtcg tcggtaagat tggcagctac ctgccagata
     5101 aggcgtaaac gccttatctg gcatacgttc agcacattac ccccaggtcg gataagacgc
     5161 ggcaagcgtc gcatccggca atgtctgccc agttctggaa tcgcgcttcc ataaaacaaa
     5221 aaatccctgt caccatcctc tacattctct tgtttagcgt ttttctacgt ttatgctaca
     5281 tacgtgaagt tatgacgcat aaagaatacg atttctttac cgccgttcat cgtactaagg
     5341 ggaaaaaatg attgcgattg ccgacatctt gcaagcagga gaaaagttaa ctgctgtggc
     5401 accttttctg gcgggtattc agaacgagga acaatacacc caggcgctgg aactggtaga
     5461 tcatctgctg ctcaacgatc ctgaaaaccc cttgctggat ctggtgtgtg ccaaaataac
     5521 cgcgtgggaa gaatcagcgc ccgaatttgc tgaatttaat gccatggcgc aagccatgcc
     5581 tggcggtata gctgtgattc gtacccttat ggatcaatat ggtttaaccc tttccgatct
     5641 tccggaaatt ggcagtaaat cgatggtgtc acgcgttttg agcgggaaga ggaaattaac
     5701 gctggaacac gctaaaaaat tggcaacgcg attcggcatt tctcccgcct tgtttattga
     5761 ttaacgcgac gtgccggatg cgttgcatcc ggcgcttcaa ataacgattt aaatctccag
     5821 cgccagccgt gttccctggg caattgcccg tcgtgcgtcc agctccatag ctacatcgca
     5881 gccgccaatt aaatgcacag ttttcccgct atcaatcagc ggttgcgcca atgcgcggtt
     5941 tggctcttgc cctgcgcaga tcaccacatt gtccactgct aatacctgcg gttcgccgtt
     6001 gatcaccaca tgcagcccgt catcgtcgat cttctgataa cttacacctg ggatcatttt
     6061 cacgccgcgc gagagtaggg tggtgcgatg gatccagccg gtggttttgc ctaacccctg
     6121 tcccggtttg ctggctttgc gctggagcat cacaatctgc cgtgggctac gagggatctg
     6181 cattccctgc gggcttaagc caccagcctg ttgtaggctg ctgtcgatcc cccactcatt
     6241 acagaacccg gcgatattct ggctggtgga ttcgcccggc tgacttaaat acatcgccgt
     6301 gtcaaagcca atcccaccac aaccgatgat ggcaactttg ttgcccaccg gcgctttgtc
     6361 gcgcagtaca tcgagataac tcaatacctt cggatgatcg atcccgttga taggcggaac
     6421 gcgcggcacg atcccactgg cgaggatcgt ttcatcgaac gcctgtaact gatccgccgt
     6481 cacggtgtga ttgagtttta gcgtcacgcc cgttacttcg atcatccggc ggtagtagcg
     6541 cagcgtttcg taaaactcct ctttgccggg gatctgtttg gcgatattaa actgcccgcc
     6601 aatctcgcta tgagcgtcaa acaatgttac ctgatgccca cgcgccgccg cgttaatggc
     6661 aaacgccagc ccagcaggtc ccgcaccgac caccgccaga ttttttttct gcacggcagg
     6721 aaggattggc attttggttt catggcaggc gcgaggattc accaggcacg aggtgacttt
     6781 gccaacgaag atctgatcga gacaggcctg gttgcagcca atacaagtgt tgatctcatc
     6841 ggctcgtccc gattgcgctt ttgacagcag ctccgcatca gcgagaaacg gtcgcgccat
     6901 cgataccatg tccgcatcac cgcgcgagag aatgtcgtcg gcaacctgcg gatcgttaat
     6961 ccggttggtg gtcaccagtg gcagcgaaac gtggcctttc agtttgcgcg tgacccagct
     7021 aaatgcgccg cgcggcacgg gcgtggcaat ggtcggaata cgtgcttcat gccagccaat
     7081 gccggtgttg ataatcgttg cgcccgccgc ttcaatggcc tgcgccagct ctaccgtttc
     7141 ggcaaaagta ccgccgcctt cgaccaggtc gagcatcgac aaacggtaga taataatgaa
     7201 gtcgttgccg acacgttcgc gcaccgcacg cactacttct acggcaaacc gcatccggtt
     7261 gcggtaatcg ccgccccact ggtcgctacg ctggttggtg cgcagggtaa gaaactcgtt
     7321 gatcaaatac ccttcggacc ccattacctc tacgccatcg tatcctgctt ctcgcgccag
     7381 ttgcgcacag cgggcgaaat tgtcgatcag ttgcagaatc tcttcatggg tgagttcatg
     7441 gggaatgaaa cggttaattg gtgcctgcaa cggagagggt gcaaccaggt gcggctggta
     7501 gctgtagcgt ccggtatgca aaatttgtag ggcaattttg ccgccttcct gatgtacggc
     7561 ttcggtaatg gtgcgatggt gtgggatctg gttggcgtca ttgagcattg caccgccttc
     7621 catgccaacg cctgttaaat ctggtgcgat accgccgctg acaatcaacg ccacgccgtg
     7681 acgggcgcgt tcggcataaa acgctgccag ccgttcagcg ccgtccgggt attcctccag
     7741 cccggtgtgc attgagccca tcaacacgcg gttttttaac gtggtaaaac ctaaatccag
     7801 cggggcgaac agcgacgggt agctcataaa attgtccagt atgtaaaata attgttatgt
     7861 ggtcggatga gttctaattt agccgtcgcc ggagaaaagg gaaaaggaga gtgcggggtt
     7921 tgtgatggga ttcaaaaaga atacgcccgg tggtaatgcc ggatgcaacg cttgccgcat
     7981 cttatccggc ctgggggatt gcacatgtag ggcggataag gcgttcacgc cgcatccgcc
     8041 agccgttgca aattactgct tacggaaaaa atcgttatac aacatataca aatgcgcggc
     8101 actccaggag aaatttggtg cgccttgctg cgcgccagtg agcgggttgt aattctcctg
     8161 aatcgggcca tcggcggtta accctttggc gtgccggaag aacgtatccg ccagcttcag
     8221 ggcatcatca cgataaccgt aacgctccat tcctttcaga ccaaaccaga actgatccac
     8281 ccatacgcgc ccgcgccagt agatatctgc gccaaaggcc gggttggtta acgctgccgt
     8341 tcccagcggg acaaaggtgt tgaactcttt tggatcgagc atcaccttca ccaccgcgtc
     8401 ggcgttggcc tgcgttgctg caccgttaaa cagcggtgac cagccttccg gccctttacc
     8461 gcgctcaaca atcggtttgc ccgcacagcc gttcgccagc ggtttatctt caatacgcac
     8521 gtcatagtag aactgtgtag tcgggtcgaa catacaggtg ttgatgtagt ccgcgagctg
     8581 ttgtgctaac tgacgatagc gtttggcctc ttccggctta ccgagaatcg tcgccatctc
     8641 cgccagataa tggttatcgc tgtacatata gctggcctga tccaccgact cctgcaatag
     8701 cgagtagccc agcaacgttc cgtcctgact gcggttctcg gcgaatttca ccgtccagtc
     8761 gctacgtttg ccgccgttag cgacatattt atccaactgt tctttgtcga taaacccaaa
     8821 gacggcggca tcatcacgac ctgattccca cgacgccgca acctgtgccg ggatttccag
     8881 actgtcgtac tggcctttct ccaccacgcg ggtgtagttg ttcagcccag acagcgtctc
     8941 ctctttggcg ccttttttca ctgtaaacag catctcgcca ttctcagtat tgtgggcttt
     9001 atcgcgggtc gcgccatatt ccggcacgcc gttgccgttg tgatcgcggt tacgtagcca
     9061 ccagtcgtga taggccacca gtttcgggta catctctgcc agccaggttt tatcctgggt
     9121 gacgttgtac acttccatca ccgaccaggc ggcaaggctg ggtttggtgt tacgttcgtt
     9181 ccagttgccg ccatcgccgc cacgctcggg gctaaggttc cacgctatca ggtcggggac
     9241 aaagcccaca tcctgcggac gcacgctgtc gcctggctgg atctgccagg agaagaccgc
     9301 gcggatattc tctttggcga tatccggatt gaaatgcgcc atcgcaaacg cctgcttcca
     9361 ggtatcccac ggccaggtct gattgccgga gaaccagcgc ccggtcaccg acggcgtgac
     9421 ggtgttatat ttcaccgcac cgcccggcga tcgccagtta ccgttgagcg tttcgatggc
     9481 tttcaccgcg acgcgcgtct gttccggcgt cgcatccgga ttggttaacc ctttcttcag
     9541 atactcttcc cagcgttgct gcgaggcggt gagataaaac gccggacgcg ccagaatatc
     9601 gcggatctgc atttgctctt tgctaacttc ctgagcggtc agcaggtggg agtaggtggt
     9661 atagagcgtg gccgaaccgt tgatatgcgc cttactggta aagcgattac cgttgatttc
     9721 agtctgcact ggcagggatt tatgcacctg atattctgat tcaccggagg tcagcagatc
     9781 ccaggtggcg cgcactttgc caaaggtgac tttcaggcca tcacgggtgg cgctgatttt
     9841 gcgctgatag tcagggtatt cgccagcaat ggttttatcg gaaagcggct tcccttcttt
     9901 cgcttccagt ttttccagca gctcgccgtc ccacaccaga tccagcggtt tattgctgat
     9961 gattttagtt tccagtagtg acgtgcgcgg cgtggcgaag cgaagagtca tttcgaccag
    10021 cacatctttt gctgtcagtt tttgcaccag cgcgccggga atactgtatg cctccagcgt
    10081 gaagtcgact ttcttgccgt cttgccagac ggtcaggcgg tcgaaattgc tggccataaa
    10141 gttgatgtac tcttccgtca acaacgcaac gcctggaaag ccgcccatag tgttcgggcc
    10201 gtctggcaac agatgaccat gccaggctcc gagatcgaaa aacggattaa aacgctggtg
    10261 atcgtcgtaa tcgtaatcct tcatgtactg cggcgcgcca gtacggttaa tcacgttttt
    10321 ataattgtcg gcgttagcgg catgggcgga aaaacttatc agcagagcgc aggttactgg
    10381 cgttaaaata gttttgattt tcatgaaatt acagtcctta ttaccagtag aaatattgca
    10441 tcaggaagag ttggtcgctg ggagcgtgag aggcattttt catgtagaag cggctatcaa
    10501 aagccgttgc gaaacgtccg ccgccgtatt cgtaccagat accaaactgc atgaaagagg
    10561 tggtttcatc gtcagcgtgg tcagggcgat gtttggcgta ggtgtaagcg gtggagagat
    10621 aaatgccctt taacgcttca tacatggcgc tcaccatcat cccgctttca cttcccgata
    10681 cgtccagccc gtcggcgcgt ccggtatggt gccaggcgcg agcggcaaag ttaggggtaa
    10741 gcaggcccgt cagatagagt tggcctgtgc cgtcggtgat ttccagaccg ttcatccagg
    10801 tgatgccttc ggcaaggtca tactggatgt aaccgttcat cattgccgga taggtgtatt
    10861 tgtcgtcgta gcgatcgtac ttgccaaaat gtagccaggc tttgctttcg tcatgatgtc
    10921 cggcaggtgt ggcggtaacg ctataacgaa agttgccgct taagttctgc accttcagcg
    10981 cggtcataat gtcgcgggta ttgggaatga cgtagcccag atcgggcgtg aaatcgcccc
    11041 accattgcag gtcatccagc gaggaatcct gacgcagact gagcatgatt tcggtgccat
    11101 cattggtacg ataaccgcca tagaaacggt taatcccacc ttcgaaaccg ccccagctat
    11161 ggtcaggcac ccaggcatta ccgtcgtggt cggcctgcac ggtccagcct tcgccgtaaa
    11221 tcaggccgaa ccaggatccg tacttcatct ccaggccacc ttcaatatag gtgccgtcgc
    11281 tgtatttatg tttattctca ccctctaaat ccatataccc accaacccct aattcgccgt
    11341 aaaaacgaaa tgaaggagaa gatgtcgagg tcattgcggg ggtttttgtg tcgtcatgtt
    11401 ctgccggacg tgcttcatca gcggtcacag aaaaggcgta gcaaaacggc agcgcaagta
    11461 atgctctgca tggtgcagtg attaatttca ttgtgttaat ccatgagcta aagaaaa
//
LOCUS       DAPHCV010000130        11420 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.131, whole genome shotgun sequence.
ACCESSION   DAPHCV010000130 DAPHCV010000000
VERSION     DAPHCV010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.131"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            194..553
                     /locus_tag="RH033_003935"
     CDS             194..553
                     /locus_tag="RH033_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HDV2993049.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPATAADITARADQEGWTPGFTEKMVGWAKKMESGERIVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(673..852)
                     /locus_tag="RH033_003936"
     CDS             complement(673..852)
                     /locus_tag="RH033_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HDV2993050.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HAGERIVARFEDEDE"
     gene            926..2287
                     /gene="pabB"
                     /locus_tag="RH033_003937"
     CDS             926..2287
                     /gene="pabB"
                     /locus_tag="RH033_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HDV2993051.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPAHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAQQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGVILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLCAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2291..2869
                     /locus_tag="RH033_003938"
     CDS             2291..2869
                     /locus_tag="RH033_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HDV2993052.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            3053..4417
                     /gene="sdaA"
                     /locus_tag="RH033_003939"
     CDS             3053..4417
                     /gene="sdaA"
                     /locus_tag="RH033_003939"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDV2993053.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4548..6146
                     /locus_tag="RH033_003940"
     CDS             4548..6146
                     /locus_tag="RH033_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDV2993054.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDHALIKGSPVLIQWTPAAGSSK
                     AGVMEMINIDLLAAMLLEPQLPQISSASLTVDDRHLLYGNGLVDSLPQPENNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(6150..7706)
                     /gene="yoaE"
                     /locus_tag="RH033_003941"
     CDS             complement(6150..7706)
                     /gene="yoaE"
                     /locus_tag="RH033_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HDV2993055.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCSGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            8169..9140
                     /gene="manX"
                     /locus_tag="RH033_003942"
     CDS             8169..9140
                     /gene="manX"
                     /locus_tag="RH033_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDV2993056.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKATPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9203..10003
                     /gene="manY"
                     /locus_tag="RH033_003943"
     CDS             9203..10003
                     /gene="manY"
                     /locus_tag="RH033_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HDV2993057.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            10016..10867
                     /gene="manZ"
                     /locus_tag="RH033_003944"
     CDS             10016..10867
                     /gene="manZ"
                     /locus_tag="RH033_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HDV2993058.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10922..11380
                     /locus_tag="RH033_003945"
     CDS             10922..11380
                     /locus_tag="RH033_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HDV2993059.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         2796 a         2882 c         2872 g         2870 t
ORIGIN      
        1 aatgccgttg tgttcgccct tcgattagca tctctggttt tctttcttag ctgaacaatt
       61 tggttatagc gtgcggactg accgttaatt ttctccatct tactggataa cacaccatga
      121 gctgctacct ttaaaacacc tgaatgtcaa caggttaact cgtgccatat cgttataatc
      181 cctttgatat tagatgcaaa ttaaggtcat atatagcctt atcgataaca tggttaattt
      241 taaggataag aatatgcctg ctgtaataga taaagccctg gatttcattg gtgccatgga
      301 tgtatcagcg ccaacaccaa gttcgatgaa tgaaagcacg gcgaagggaa tctttaaata
      361 tttgaaagaa ctgggagtac ccgcgactgc cgctgatatt acggcgcgag ctgaccagga
      421 aggctggacc ccggggttca cggaaaaaat ggttggatgg gcaaaaaaaa tggagtctgg
      481 tgaacgcatt gtgattaaaa atcctgaata cttttcaaca tatatgcagg aagaactaaa
      541 agcactggtc taagttaaat ttacatcagc ataaatgggt caggacgctt ttaatcacat
      601 atgaaaagcg tcctttttct cacaatcaaa taaccattgg cttctataac gaaagcgata
      661 cggataagtg tgttactcgt cttcatcctc aaaacgcgcc acgattcgct cgccggcatg
      721 gttggcacgc agctcttctg ccaccaacgc gattgcctgg ccgctgctca tcccttgagc
      781 catcagttcc tggatccgct cgacagcttt ttgctgctgt tcatgggtga gtgaaggtaa
      841 acctgcaaac attgttaact cctgctaaat tgttggcgct aattatttca tgctacccgg
      901 cacatagcca gtagagtcag gactgatgaa gacgttatct cccgctgtga ttactttacc
      961 ctggcgtcag gacgccgctg aattttattt ctcccgctta agccacctgc cgtgggcgat
     1021 gcttttacac tccggctatg ccgatcatcc atatagccgc tttgatattg tggtcgccga
     1081 gccaatttgc actttaacca ctttcggtaa agaaaccgtt gttagtgaaa gcgaaaaacg
     1141 cacaacgacc actgatgacc cgctacaggt gctccagcag gtgctggatc gcgcggacat
     1201 tcgcccagcg cataacgaag atttgccatt tcagggcggc gcgctggggt tgtttggcta
     1261 cgatctgggc cgccgttttg agtcactgcc agaaattgcg cagcaagata tcgttctgcc
     1321 ggatatggca gtgggtatct acgattgggc gctcattgtc gaccaccagc gtcatacagt
     1381 ttctttgctg agtcataatg atgtcaatgc ccgtcgggcc tggctggaaa gccagcaatt
     1441 ctcgccgcag gaagatttca cgctcacttc cgactggcaa tccaatatga cccgcgagca
     1501 gtacggcgaa aaatttcgcc aggtacagga atatctgcac agcggtgatt gctatcaggt
     1561 gaatctcgcc cagcgttttc atgcgaccta ttctggcgat gaatggcagg cattccttca
     1621 gcttaatcag gccaaccgcg cgccatttag cgctttttta cgtcttgaac agggcgtgat
     1681 tttaagcctt tcgccagagc ggtttattct ttgtgataat agtgaaatcc agacccgccc
     1741 gattaaaggc acgctaccac gcctgcccga tcctcaggaa gatagcaaac aagcagagaa
     1801 actggcgaac tcagcgaaag atcgtgccga aaatctgatg attgtcgatt taatgcgtaa
     1861 tgatatcggt cgtgttgccg tagcaggttc ggtaaaagta ccagagctct tcgtggtgga
     1921 acccttccct gccgtgcatc atctggtcag caccataacg gcgcggctac cagaacagtt
     1981 acacgccagc gatctgctgt gcgcagcttt tcctggtggc tcaataaccg gggctccgaa
     2041 agtacgggct atggaaatta tcgacgaact ggaaccgcag cgacgtaatg cctggtgcgg
     2101 cagcattggc tatttgagct tttgcggcaa catggatacc agcattacta tccgcactct
     2161 gactgccatt aacggacaaa tatactgctc tgcgggcggt ggaattgtcg ccgatagcca
     2221 ggaagaagcg gaatatcagg aaacttttga taaagttaat aagatattac gccaactgga
     2281 gaagtaagac gtggaatacc gtagcctgac gcttgatgat tttttatcgc gctttcaact
     2341 tttgcgcccg caaattaacc gggaaaccct aaatcatcgt caggctgccg tgttaatccc
     2401 catcgtccgt cgaccgcaac cggggttgtt gctgactcag cgttcaattc atctgcgtaa
     2461 acacgctgga caagtggcat tccctggagg cgcagtcgat gacacggatg catcagttat
     2521 cgccgccgcg ctgcgcgaag ctgaagaaga ggtcgctata ccgccttccg ccgttgaagt
     2581 tatcggcgta ctgccgcccg tcgatagcgt aactggctac caggtaaccc cggtggtcgg
     2641 cattatcccg cccgatctgc cgtatcgcgc cagtgaagat gaagtctcgg cggtgtttga
     2701 aatgccgctc gcccaggcat tacatctggg tcgttatcac cctttagata tctaccgccg
     2761 tggcgattca catcgggtat ggctgtcctg gtatgaacag tattttgtat ggggaatgac
     2821 cgcaggcata attcgtgagc tggcgctgca aattggtgtg aaaccctgac tatacttatc
     2881 tttacatcta caaaacacta cttgagacaa tcatcgcaat attagttaaa tcgcggtttt
     2941 tgattagttt aattcatgtg aatagttaag ccagtcgccg cgttccctct tacactatgc
     3001 gctgttatta gttcgttact ggaagtccag tcaccttgtc aggagtatta tcgtgattag
     3061 tctattcgac atgtttaagg tggggattgg tccctcatct tcccataccg tagggcctat
     3121 gaaggcgggt aaacagttcg tcgatgatct ggtcgaaaaa ggcttactgg atagcgttac
     3181 tcgcgttgcc gtggacgttt atggttcact gtcgctgacg ggtaaaggcc accacaccga
     3241 tatcgccatt attatgggtc ttgcaggtaa cgaacctgcc accgtggata tcgacagtat
     3301 tcccggtttt attcgcgacg tagaagagcg cgaacgtctg ctgctggcac agggacggca
     3361 tgaagtggat ttcccgcgcg acaacgggat gcgttttcat aacggcaacc tgccgctgca
     3421 tgaaaacggt atgcaaatcc acgcctataa cggcgatgaa gtcgtctaca gcaaaactta
     3481 ttattccatc ggcggcggtt ttatcgtcga tgaagaacac tttggtcagg atgccgccaa
     3541 cgaagtgagc gtgccgtatc cgttcaaatc tgccaccgaa ctgctggcgt actgcaatga
     3601 aaccggctat tcgctttctg gtctcgctat gcagaacgaa ctggcgctgc acagcaagaa
     3661 agagatcgac gagtatttcg ctcatgtctg gcaaaccatg caggcatgta tcgatcgcgg
     3721 gatgaacacc gaaggcgtac tgccaggccc gctgcgcgtg ccgcgtcgtg cgtcggccct
     3781 gcgccggatg ttagtttcca gcgataaact gtctaacgat ccgatgaatg tcattgactg
     3841 ggtaaacatg tttgcgctgg cagttaacga agaaaacgcc gccggtggtc gtgtggtaac
     3901 agcgccaacc aacggtgctt gtggcattgt tccggcagtg ctggcttatt atgaccactt
     3961 tattgaatcg gttagcccgg acatctatac ccgttacttt atggcagcgg gcgcgattgg
     4021 tgcactgtat aaaatgaacg cgtctatttc cggtgcggaa gtcggttgcc agggcgaagt
     4081 gggtgttgcc tgttcaatgg ctgctgcggg tcttgcagaa ctgctgggcg gtagcccgga
     4141 acaggtttgc gtggcggcgg aaattggcat ggaacacaac cttggtttaa cctgcgaccc
     4201 ggttgcaggg caggttcagg tgccgtgcat tgagcgtaat gccattgcct ctgtgaaggc
     4261 gattaacgcc gcacggatgg ctctgcgccg caccagtgca ccgcgcgtct cgctggataa
     4321 ggtcatcgaa acgatgtacg aaaccggtaa ggacatgaac gccaaatacc gcgaaacctc
     4381 acgcggtggt ctggcaatca aagtccagtg tgactaatac ttcttactcg cccatctgca
     4441 acggatgggc gaatttatcc ccgctttctc gtctgctgta atattcccca ctacacttcc
     4501 actgttgcgt caggcgtttg tcgccatacg cttacagggt ggcccgcatg caaaaagcac
     4561 aacggatcat taaaacctat cgccgtaatc gaatgattgt ttgtacgatt tgcgcactcg
     4621 ttacgctcgc ttcgaccctg agcgtgcgat ttatttcaca gcgtaactta aatcaacaac
     4681 gggtagtaca attcgccaat cacgctgtag aggaattaga taaagtactg cttcccctac
     4741 aggcaggtag cgaagtcttg cttccgctga ttggtctgcc ctgctctgtc gcccatttgc
     4801 cattacgtaa acaggcggca aaactccaaa ctgtgcgatc cattggcctg gtgcaagacg
     4861 gcacacttta ttgctccagc atttttggtt atcgcaatgt gcccgtcgtg gacattctgg
     4921 ctgaacttcc tgcaccgcaa ccacttttac gcctgacgac cgaccatgcc ctgattaaag
     4981 gcagtccggt tttgattcaa tggacgccag cagcgggcag tagcaaagct ggggttatgg
     5041 agatgattaa catcgactta ctggcggcaa tgctgcttga gccacaactg ccgcaaatca
     5101 gtagcgccag cctgacggtg gacgatcggc atttgctcta tggtaatggg ctggtagatt
     5161 cccttccgca acctgaaaac aatgaaaact atcaggtttc ttcgcaacgc tttcctttta
     5221 ccattaacgt taatggcccg ggggctacgg cgctggcatg gcactatctt ccaacacaat
     5281 taccgctggc ggtgctgcta agtttactgg tgggctatat cgcctggctg gcgaccgctt
     5341 accggatgag tttttcccgc gaaatcaatc tgggcctggc gcaacatgag ttcgaattgt
     5401 tctgccagcc tttgcttaat gcgcgcagcc agcaatgtat tggcgtagag attttgctgc
     5461 gctggaacaa tccgcgtcag ggctggattt cgccggatgt gtttattcct atcgcggaag
     5521 aacatcattt aattgtgcca ctgacccgct atgtgatggc agaaaccatt cgtcagcgcc
     5581 atgttttccc gatgagtagt cagtttcatg ttggcattaa cgtcgcaccc agccattttc
     5641 gccgtggtgt gctgataaaa gatctcaatc agtactggtt tagcgctcac ccgattcagc
     5701 aactgatcct cgaaatcacc gaacgcgatg ccttactgga tgttgattat cggattgccc
     5761 gcgagctaca tcgtaaaaac gtcaaactgg cgattgatga cttcggcacc ggcaatagtt
     5821 cattttcctg gcttgaaaca ttacgtcctg acgtgctgaa aattgataag tcatttaccg
     5881 cagctatagg ttctgacgcg gttaactcga cggtgaccga tatcatcatc gcactggggc
     5941 aaagactgaa tattgaactg gtggcggagg gtgtggaaac gcaagaacag gcgaaatatt
     6001 tgcgccgtca tggcgtccat attttgcaag ggtatttgta cgcacagccg atgccactac
     6061 gtgattttcc caaatggctg gcgggcagcc aaccgccgcc cgcccggcat aatggacata
     6121 tcacacccat tatgccgtta cgttaacgct tactcatctt catcgtgcgc cggttgctct
     6181 ttaacaatgc gaaccagatc aacacgataa tcattggctt caatgatggt gatatgcagt
     6241 ggccctacat caatcacatc gcccacacgg ggaatgtgac catttgccga gatcacgagg
     6301 cccgcgaccg tcgcgatatc atcgtcatcg gcaaggtgct caacatcaag cgcctgctgc
     6361 aaggcatgca aatctgtacc gccttttacc agccagccgt caccgtcggt aatgatttcc
     6421 ggcgtttcgt cagcgtccgg gaattcaccc gcaatggctt ccagcacatc cagcggcgtg
     6481 accaagcctt gtaccacacc aaactcgttg gtcacgataa caaagctccc gcgagcacga
     6541 cgcagcacgc ccagcaggtt gatcggatcg agggtttccg ggacgataat cgccggagac
     6601 gccgaagcaa tcgccgccac atcaacgccc tcttccagcg ccaccagcag ttctttagca
     6661 cgtacgatac caatgatttc atccagttca ccgctacata ccgggaacag actgtgcggt
     6721 gaagagagca gttgctcgcg gatttcatcg accccgagat tagcatcaac ccagcttatt
     6781 tcaccgcgcg gcgtcatgat cccgcgcaga gaacgcgacg ccagcgtcag tacgccgtta
     6841 atcatgtaac gttcttcttc ggcaaatgca ccttccggga tcggcatcgg catcgggtta
     6901 tcggcatcgt gctggacatt ggcctgacgt ttcccgccca tcaaacgcag gatggcatcg
     6961 gcagtacgcg ctcgcagcgg caaagtcgac tggtgacgaa taaagttgcg acgcgcaatc
     7021 tggttaaaca cttcgatgat gatcgagaag ccaatcgcgg catacaggta acctttcgga
     7081 atgtggaaac cgaaaccttc tgccaccaga ctcagaccaa tcattaacag gaagctcaga
     7141 cagagcacca ccaccgttgg atgctggtta acaaatcgcg tcagcggttt cgatgccagc
     7201 agcataactg ccatcgcaat cactaccgcc gccatcatca ccggcaaatg gttaaccatc
     7261 cctactgcag taattaccgc atccaacgag aagacggcgt caaggatgac aatctgtgtg
     7321 acgaccaccc agaaactggc gtagccttta ccgtggccgg aatcatgatc gcggttttcc
     7381 agccgttcat gcagttcggt tgttgctttg aacagcaaga atatgccccc gaacaacata
     7441 atcaggtcgc gtccggagaa ggagaaatcc atgacggtaa atagcggttt ggtcagcgtg
     7501 accatccatg aaatcagcga cagcagcccc agacgcataa tcagcgccag tgataatccc
     7561 agcaaacgcg ctttatcgcg ttgttttggc ggcagtttgt cagcaagaat ggcaatgaag
     7621 accaggttat cgatacccag cacaatttcg agaacaacaa gcgtgagtag ccccgcccaa
     7681 attgaggggt ccattaagaa ttccatgaca agctcctgct taaggaatag ctattcgacg
     7741 ccagaaataa tgcaggcgta acgacaaaat gcaaacgaaa ggtgcggcat agagtgccag
     7801 aaaggcaggc gttaaaaggc ctgatgctga aatgacgtcg gtgacgatcc atactgcggg
     7861 ctactgccct atactccatg gttgttaaac gggagttaaa catatcagag acgcctctga
     7921 tttggcaaag atttaccttc ctttgcaaac gaatgtgaca aggatatttt acctttcgaa
     7981 atttctgcta atcgaaagtt aaattacgga tcttcatcac ataaaataat tttttcgata
     8041 tctaaaataa atcgcgaaac gcaggggttt ttggttgtag cccttatctg aatcgattcg
     8101 attgtggacg acgattcaaa aatacatctg gcacgttgag gtgttaacga taataaagga
     8161 ggtagcaagt gaccattgct attgttatag gcacacatgg ttgggctgca gagcagttgc
     8221 ttaaaacggc agaaatgctg ttaggcgagc aggaaaacgt cggctggatc gatttcgttc
     8281 caggtgaaaa tgccgaaacg ctgattgaaa agtacaacgc tcagttggca aaactcgaca
     8341 ccactaaagg cgtgctgttt ctcgttgata catggggagg cagcccgttc aatgctgcca
     8401 gccgcattgt cgtcgacaaa gagcattatg aagtcattgc aggcgttaac attccaatgc
     8461 tcgtggaaac gttaatggcc cgtgatgatg acccaagctt tgatgaactg gtggcgctgg
     8521 cagtagaaac aggtcgtgaa ggcgtgaaag cactgaaagc caaaccggtt gaaaaagccg
     8581 cgccagcacc cgctgccgca gcaccaaaag cgactccaac tccggcaaaa ccaatgggac
     8641 caaacgacta catggttatt ggccttgcgc gtatcgacga ccgtctgata cacggtcagg
     8701 tcgccacccg ctggaccaaa gaaaccaatg tctcccgtat tattgttgtt agtgatgaag
     8761 tggctgcgga taccgttcgt aagacactgc tcacccaggt tgcacctccg ggcgtaacag
     8821 cacacgtagt tgatgttgcc aaaatgattc gcgtctacaa caacccgaaa tatgctggcg
     8881 aacgcgtaat gctgttattt accaacccaa cagatgtaga gcgtctcgtt gaaggcggcg
     8941 tgaaaatcac ctctgttaac gtcggtggta tggcattccg tcagggtaaa acccaggtga
     9001 ataacgcggt ttcggttgat gaaaaagata tcgaggcgtt caagaaactg aatgcgcgcg
     9061 gtattgagct ggaagtccgt aaggtttcca ccgatccgaa actgaaaatg atggatctga
     9121 tcagcaaaat cgataagtaa cgtattgtgt tgattatcac tcagttttca cacttaagtc
     9181 ttacgtaaac aggagaagta caatggagat taccactctt caaattgtgc tggtatttat
     9241 cgtagcctgt atcgcaggta tgggatcaat cctcgatgaa tttcagtttc accgtccgct
     9301 aatcgcgtgt accctggtgg gtatcgttct tggcgatatg aaaaccggta ttattatcgg
     9361 tggtacgctg gaaatgatcg cgctgggctg gatgaacatc ggtgctgcag ttgcgcctga
     9421 cgccgctctg gcttccatca tttctactat tctggttatc gcaggtcatc agagcattgg
     9481 tgcaggtatc gcactggcaa tccctctggc cgctgcgggc caggtactga ccatcatcgt
     9541 tcgtactatt accgttgcct tccagcacgc tgcggataag gctgctgata acggcaacct
     9601 gacagcgatt tcctggatcc acgtttcttc tctgttcctg caagcaatgc gtgtggctat
     9661 tccggccgtc atcgttgcgc tgtctgttgg taccagcgaa gtccagaaca tgctgaatgc
     9721 gattccggaa gtggtgacca atggtctgaa tatcgccggt ggcatgatcg tggtggttgg
     9781 ttatgcgatg gttatcaaca tgatgcgtgc tggctacctg atgccgttct tctacctcgg
     9841 cttcgtaacc gcagcattca ccaactttaa cctggttgct ctgggtgtga ttggtactgt
     9901 tatggcagtg ctctacatcc aacttagccc gaaatacaac cgcgtagccg gtgcgcctgc
     9961 tcaggcagct ggtaacaacg atctcgataa cgaactggac taacaggtga gcgaaatggt
    10021 tgatacaact caaactacca ccgagaaaaa actcactcaa agtgatattc gtggcgtctt
    10081 cctgcgttct aacctcttcc agggttcatg gaacttcgaa cgtatgcagg cactgggttt
    10141 ctgcttctct atggtaccgg caattcgtcg cctctaccct gagaacaacg aagctcgtaa
    10201 acaagctatt cgccgtcacc tggagttctt taacacccag ccgttcgtgg ctgcgccgat
    10261 tctcggcgta accctggcgc tggaagaaca gcgtgctaat ggcgcagaga tcgatgacgg
    10321 tgctatcaac ggtatcaaag tcggtttgat ggggccgctg gctggtgtag gcgacccgat
    10381 cttctgggga accgtacgtc cggtatttgc agcactgggg gccggtatcg caatgagcgg
    10441 cagcctgtta ggtccgctgc tgttcttcat cttgtttaac ctggtgcgtc tggcaacccg
    10501 ttactacggc gtagcgtatg gttactccaa aggtatcgat atcgttaaag atatgggtgg
    10561 tggcttcctg caaaaactga cggaaggggc gtctatcctc ggcctgtttg tcatgggggc
    10621 attagttaac aagtggacgc atgtcaacat cccgctggtt gtctctcgca ttactgacca
    10681 gacgggcaaa gaacacgtta ccactgtcca gactattctt gaccagttaa tgccaggcct
    10741 ggtaccactg ctgctgacct ttgcttgtat gtggctgttg cgcaaaaaag ttaacccgct
    10801 gtggatcatc gttggcttct tcgtcatcgg tatcgctggt tacgcttgcg gcctgctggg
    10861 actgtaagac tgttgtacac taccggggcc ttttggcccc gtttttttat ctggaggatt
    10921 aatgacaatc acggacctgg tactgattct tttcatcgcc gcactcctgg ccttcgcgat
    10981 ctacgatcag ttcatcatgc cccgccgtaa cggccccacc ctgctggcaa ttcctttgct
    11041 ccggcgtggt cgcatcgata gcgttatctt cgtcggattg attgtcattc ttatctataa
    11101 caacgtcacg aatcatggcg cgctaataac aacatggtta ttaagtgcac tggctctgat
    11161 gggtttttat atattctgga tccgcgttcc gaagatcatc tttaaacaaa aaggtttttt
    11221 cttcgccaat gtctggattg aatatagccg aatcaaagcg atgaacttgt cggaagatgg
    11281 cgtgttggtg atgcaattag aacagcgccg gctgttaatc cgcgttcgaa atatcgacga
    11341 tctggaaaaa atttataagc ttctcgtttc aactcaataa gttatgaatt tagccaaagc
    11401 tatgtttagt gtatttttaa
//
LOCUS       DAPHCV010000131        11312 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.132, whole genome shotgun sequence.
ACCESSION   DAPHCV010000131 DAPHCV010000000
VERSION     DAPHCV010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.132"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            198..1769
                     /gene="garD"
                     /locus_tag="RH033_003946"
     CDS             198..1769
                     /gene="garD"
                     /locus_tag="RH033_003946"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HDV2993060.1"
                     /translation="MANIKIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVQEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAECHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1918..2253
                     /gene="prlF"
                     /locus_tag="RH033_003947"
     CDS             1918..2253
                     /gene="prlF"
                     /locus_tag="RH033_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HDV2993061.1"
                     /translation="MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFDIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2253..2717
                     /gene="yhaV"
                     /locus_tag="RH033_003948"
     CDS             2253..2717
                     /gene="yhaV"
                     /locus_tag="RH033_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HDV2993062.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(2772..3581)
                     /gene="agaR"
                     /locus_tag="RH033_003949"
     CDS             complement(2772..3581)
                     /gene="agaR"
                     /locus_tag="RH033_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HDV2993063.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3830..5110
                     /gene="kbaZ"
                     /locus_tag="RH033_003950"
     CDS             3830..5110
                     /gene="kbaZ"
                     /locus_tag="RH033_003950"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HDV2993064.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLVYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTLRELIINHIQDILAQYHTACEGQ"
     gene            5133..5606
                     /gene="agaV"
                     /locus_tag="RH033_003951"
     CDS             5133..5606
                     /gene="agaV"
                     /locus_tag="RH033_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HDV2993065.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5617..6396
                     /locus_tag="RH033_003952"
     CDS             5617..6396
                     /locus_tag="RH033_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HDV2993066.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6386..7264
                     /locus_tag="RH033_003953"
     CDS             6386..7264
                     /locus_tag="RH033_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDV2993067.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7282..7716
                     /locus_tag="RH033_003954"
     CDS             7282..7716
                     /locus_tag="RH033_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDV2993068.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKLGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7713..8846
                     /gene="nagA"
                     /locus_tag="RH033_003955"
     CDS             7713..8846
                     /gene="nagA"
                     /locus_tag="RH033_003955"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDV2993069.1"
                     /translation="MTPVLRARRLLTEEGWLDDHQLRIADGVIAEIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDALDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LERIAQRCQRGGPGAQILGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELAGVTPAEAIHMASLH
                     PARMLGVDDVLGSLKPGKRASVVALDSGLHVQQIRIQGQLASF"
     gene            9195..10349
                     /locus_tag="RH033_003956"
     CDS             9195..10349
                     /locus_tag="RH033_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HDV2993070.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLQIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMVSCLAVFAPETINSQSFRDVADRCQAILTSLGDFSEGV
                     FGYSPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQALRVIAIAAESNDVITAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10362..11222
                     /gene="kbaY"
                     /locus_tag="RH033_003957"
     CDS             10362..11222
                     /gene="kbaY"
                     /locus_tag="RH033_003957"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HDV2993071.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFDENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         2717 a         2904 c         2996 g         2695 t
ORIGIN      
        1 tgcacataag gttgcgcgct aaagcacaga tttgcgcttt accttgccgg gaggtactgc
       61 aatccctgaa atgattgaca ttgatcacat ttctgcattt aaactcctga cattcttatt
      121 tcacccaatg aagttattta tttttaaatg agaccaggtc ctcattttaa tagtccctgg
      181 ctggagaata ttgcacaatg gccaacatca aaatcagaca agaaacgcca actgcgtttt
      241 atataaaagt tcacgacaca gataatgtgg caattattgt taatgataat ggcctgaaag
      301 caggaacgcg ttttccggat gggctggaat taattgaaca tattccccag gggcataaag
      361 tcgcattgct ggacattccg gctaatggtg aaattattcg ttatggcgag gtgattggtt
      421 acgccgtgcg cgcaatccca cgcggaagct ggatcgacga atcaatggtt gtactaccgg
      481 aagcgccgcc gttacatacg ctgccactgg caaccaaagt ccaggaaccc ttaccgccgc
      541 tggaaggata cacctttgag ggctatcgca atgccgatgg cagcgtgggc accaaaaacc
      601 tgctcggcat caccaccagc gtccactgtg tggcaggcgt ggtggactac gtagtaaaaa
      661 tcattgaacg cgatctgcta ccgaaatacc cgaacgtcga tggcgtggtg gggctgaatc
      721 atttgtacgg ttgtggcgtg gcgattaacg cacccgcggc agttgtacct attcgtacca
      781 ttcacaatat ttcgctgaac cctaactttg gcggcgaagt aatggtgatt ggtctgggtt
      841 gtgaaaagtt gcagcctgaa cgcctactga ctggaacgga tgatgtgcaa gctattccag
      901 tagaaagcgc cagcattgtc agtttgcagg atgaaaagca tgtcggtttt cagtccatgg
      961 tcgaggatat tttgcaggtc gccgaatgcc atctacaaaa actgaatcaa cgtcagcgag
     1021 aaacctgtcc ggcttcagaa ctggttgtcg gcatgcagtg cggtggcagc gatgcgtttt
     1081 ctggcgtaac ggcaaacccg gcggttgggt atgcgtctga tctattggtg cgctgcggcg
     1141 caacggtgat gttttcagaa gtcacagaag tgcgtgacgc gatacatctg ctgacaccac
     1201 gcgcagtgaa cgaagaggtc ggcaaacggc tgctggagga gatggagtgg tacgataact
     1261 atctcaatat gggaaaaacc gatcgcagcg ccaacccttc gccggggaac aagaaaggcg
     1321 gtctggcaaa tgtggtggag aaggcactcg gctccattgc taaatcgggt aaaagtgcaa
     1381 ttgttgaagt gctgtcgccc ggccaacgcc cgactaaacg cggattaatt tacgccgcga
     1441 cgccagccag cgattttgtc tgtggcacgc aacaggtggc ttcgggtatc accgtgcaag
     1501 tgtttacgac cggtcgtggt acgccgtacg gcctgatggc ggtacccgtc attaaaatgg
     1561 cgacccgcac cgagctggcg aaccgctggt ttgatttaat ggatattaac gcgggcacta
     1621 tcgctaccgg cgaagaaacc attgaagaag tgggctggaa gttgtttcac tttattctcg
     1681 acgtcgccag cgggaagaag aaaactttct cggatcaatg gggactgcat aaccagctgg
     1741 cggtgtttaa cccggcaccg gtgacctgat ttcttttgga acgtccctcg caaaacatgg
     1801 ccttagtgcc atgtttttat tgtttaaagt ccacacatcc attaataatg catttgcatt
     1861 accttagttc aagcttataa tttgagcaga aaacaggctg taaaaggaca gtaaataatg
     1921 cccgctaatg ctcgctctaa cgctgtactg accactgaat caaaggtcac tatacgcgga
     1981 caaacaacta tccccgcgcc agtgcgtgag gccttaaaac tgaagcctgg cctggacagc
     2041 attcattacg aaattctgcc tggtgggcaa gtatttatgt gccgactggg agatgaacag
     2101 gaggatcata ctatgaatgc atttttgcgt tttctggatg cagatatcca gaacaacccg
     2161 caaaaaactc gtccattcga cattcaacaa ggaaagaaac ttgtcgctgg catggacgtc
     2221 aacattgacg atgagattgg cgacgacgaa taatggattt tccacaaagg gttaatggtt
     2281 gggcgctata tgctcatccc tgttttcagg aaacctacga tgctttagtt gccgaagtcg
     2341 aggcattaaa gggaaaagat cctgaaaatt atcagagaaa agctgccaca aagttattgg
     2401 cggtggtcca taaagtgatt gaggagcata tcacggtcaa tccatcatca ccggcattcc
     2461 gtcatggcaa gtcgttaggc tctgggaaaa ataaagactg gtcacgggta aaatttggcg
     2521 ctggtcgcta tcgtctcttc tttcgttaca gtgaaaaaga gaaagtcatc attctgggat
     2581 ggatgaacga tgaaaacacg ctgcgcacct acggtaaaaa aacagatgcc tataccgtat
     2641 tcagtaaaat gttaaaaaga ggacatcctc ctgccgactg ggaaagcctc acccaagaga
     2701 cagaagaaaa ccattgatgg tgttcacaat gcccgccgaa cgcattggtg ttgaccagcg
     2761 ggcatccttt ctcattcccc gaccagaatc acttcaaccc cagcctttct cagtccttcc
     2821 agactatccg caggaatgcc ttcatcaaca atgatcatgt cgatacgttg agtatcaatg
     2881 atcttatgta aactagaacg attgaactta ctggaatcgg tgaccacgat gatccgttcc
     2941 gcaacttcgc acatccgacg gtttaaacgg gcttcatctt cattatgcgt gctgacgcca
     3001 cgctccagat caatagcatc tacaccaaga aacagcatat cgaagtggta attttgcagc
     3061 gattgctcag cctgatcgcc ataaaaagat tgtgactgac ggcgcaaatg cccgccggtc
     3121 atcagcagct caacaccttc cgcttccagc aacgcattag ccacgttcat accgttggtc
     3181 atcgcaatta cgtcggtgtg cttgcgcatc agacgagcaa tctcaaaagt ggtagtcccg
     3241 gaatcgagga ttacccgatg acctggctga atcaactcaa cggcagcttt cgcaacgctg
     3301 cgtttcattg cggtgttcag cgcgctttta tcttccactg atggctcgac tgacggcgtc
     3361 gtgctatcgc agatcaacgc gccgccatag gcacgcacag cgatcccctg cttttccaga
     3421 aacgccagat cgttgcggat cgtcacagta gatacaccat acaacgccga tagatcatta
     3481 acttgcacac ttccttgctg gcgcaggcgt tgaatgatct gctctcgtcg ctcactggtg
     3541 cctgtcactc gcttctcgcc tgaagcgtcg gtattactca tagtaagtcc tttcgtaaaa
     3601 ctttcgtttc atttcgtttt gcctattaac gcctttctat taagcaaatg caagcccacc
     3661 ttacccattg gcgcaagcta ctctcgtttc agtggctttc attatgtttc ttttgtgaat
     3721 cagatcagaa aactattatc tttcgtttta tttttatctc accatgacgc agtatcaact
     3781 gaaacaaaac gaaagattaa tatcgcagta atctgaactg gagaggaaag tgaaacatct
     3841 gacagaaatg gtgagacagc acaaagcggg caaaacaaat gggatttatg ccgtttgttc
     3901 cgcacatccg ctggtgctgg aagctgcaat ccgctacgcc agtgcaaacc aaacgccgtt
     3961 actgattgaa gcaacctcca atcaggtgga ccagttcggc ggttataccg gaatgacacc
     4021 cgccgatttt cgcggctttg tttgtcagct agccgactcg ttgaatttcc cgcaggatgc
     4081 gctgattctg ggtggcgacc atctggggcc aaaccgctgg caaaacctgc ctgccgctca
     4141 ggcaatggcc aatgccgatg atttgattaa aagctacgtg gcggcaggat tcaaaaaaat
     4201 tcaccttgat tgcagcatgt cctgtcagga cgatccgatt cccttaactg atgatatcgt
     4261 ggctgaacgc gccgcccgtc tggcgaaagt ggcggaagaa acctgccgtg aacattttgg
     4321 cgaggccgat ctggtgtatg tcattggcac cgaagttccg gtgcctggcg gcgcgcatga
     4381 aaccttaagc gagctggcgg tcaccacacc ggatgccgcc cgcgccacac tggaagccca
     4441 tcgccacgcc tttgaaaagc agggcctgaa tgccatctgg ccacgcatta ttgccctggt
     4501 ggttcaaccc ggcgtcgaat tcgatcacac caacgttatt gattatcagc ccgccaaagc
     4561 gaccgcctta agccagatgg tcgaaaacta cgaaacgctg attttcgaag cgcactctac
     4621 cgattaccaa acaccgcaat cgctgcgcca gctggtgatt gaccactttg ccattctgaa
     4681 agttggcccg gcgctgacct ttgccctgcg tgaagctctg ttctctctgg cggcgattga
     4741 agaagaactg gtaccagcaa aagcctgttc tggtctgcgt caggtgctgg aaaacgtgat
     4801 gctcgaccgc ccggaatact ggcaaagcca ctaccacggt gacggcaacg cgcgtcgtct
     4861 ggcgcgtggt tacagctact cggaccgcgt gcgctattac tggccagaca gccagattga
     4921 tgacgctttc gctcatctgg ttcgtaatct ggcggattca ccaattccgc tgccgctgat
     4981 cagccagtat ctgccgctgc agtacgtgaa agttcgctcc ggcgagctgc agccaacgct
     5041 acgggaactc attatcaacc atattcagga catcctggcg cagtaccaca cagcctgtga
     5101 aggccaataa gcaaaacaaa gaggaacacg ctatgccaaa tattgtttta agccggattg
     5161 atgaacgctt gattcacggt caggttggcg ttcagtgggt cggatttgcg ggggcaaatc
     5221 tggtgctggt agccaacgat gaggttgccg aagatccggt acaacaaaac ctgatggaaa
     5281 tggtactggc agaagggatc gccgtacgtt tctggacgct gcaaaaagtt atcgacaaca
     5341 ttcatcgcgc cgccgatcga cagaaaatcc tgctggtttg taaaacacct gccgatttcc
     5401 tgacgctggt gaaaggtggc gttccggtga atcgcattaa cgttggcaat atgcactacg
     5461 ccaatggcaa gcaacaaatc gccaaaacgg tttctgtaga tgcgggcgat atcgcagcat
     5521 ttaacgacct gaaagccgct ggggtggaat gcttcgttca gggcgtcccg acagagcctg
     5581 ctgtggacct ctttaaatta ctttgaggga ttcatcatgg aaatcagtct gttgcaggca
     5641 ttcgcgttgg gcattatcgc ctttatcgct ggcctggata tgtttaacgg cctaacccat
     5701 atgcaccgcc cggtggtcct cggcccgttg gtcgggctgg tacttggcga tctgcatacc
     5761 ggtattttaa ccggcggtac gctggaactg gtgtggatgg ggctggcccc gctggcgggc
     5821 gcacagccgc ctaacgtgat tatcggtact atcgtcggca cggcgtttgc cattactact
     5881 ggcgtgaaac ccgatgtcgc ggtcggtgtc gccgtacctt tcgctgtcgc agtacagatg
     5941 gggattacct tcctgttctc ggtgatgtct ggcgtgatgt cccgctgcga ccggatggcg
     6001 gagaacgccg atactcgcgg cattgaacgc gtgaactatc tggcactgct ggcactcggc
     6061 actttctatt ttctctgcgc tttcctgcct atctacttcg gcgcagaaca tgccaaaacc
     6121 atcattgatg tcctgccgca acgattaatc gacggcctcg gcgtcgcggg cggcatcatg
     6181 ccagcaatcg gttttgccgt gctgctgaaa atcatgatga aaaacgtcta catcccctac
     6241 ttcatcctgg gctttgttgc cgcagcctgg ctcaagttac cggtgctggc tattgctgcc
     6301 gccgcgctgg cgatggcgct gatcgacctg ctgcgtaaat ctcctgaacc gactcaacct
     6361 gcggcacaga aagaggaatt tgaagatggc atctaatcaa accaccctgc cgaacgtcag
     6421 tgaaaacgaa gaaacactgc tgacaggcgt caatgaaaac gtgtatgaag atcagagcat
     6481 tggcgcagag ctgacgaaaa aagatatcaa ccgcgtcgcc tggcgttcca tgctgttaca
     6541 agcgtctttt aactacgaac gtatgcaggc ttctggctgg ttgtacggtc tgctgcctgc
     6601 tctgaaaaag atccacacta ataaacgcga cctggcgcgc gccatgaagg ggcatatggg
     6661 tttcttcaat acccatccgt ttctggtgac gtttgttatc ggcattatcc ttgcgatgga
     6721 gcgttctaag caggacgtta acagtattca gagcaccaaa attgccgtcg gtgcgccgct
     6781 cggcgggatt ggcgatgcga tgttctggct aacgctactg ccgatttgtg gcgggatagg
     6841 cgcaagcctc gcgctacaag gctctattct tggcgcagtc gtctttattg tgctgttcaa
     6901 cgtggtacac ctggggctgc gttttggtct ggcgcattat gcttaccgca tgggcgtggc
     6961 ggcgattcca ctcattaaag cgaataccaa aaaagtcggc catgctgcgt caatcgttgg
     7021 gatgacggta atcggcgcgc tggtggcaac ctatgttcgt ttaagcacca cgctggaaat
     7081 caccgctggc gacgcagtgg ttaagttaca ggctgatgtt atcgacaaac tgatgccagc
     7141 cttcttaccg ctggtctaca ccctgaccat gttctggctg gtacgccgcg gctggagtcc
     7201 gctgcgcctg attgcagtga ccgtggttct cggcatcgtc ggtaaattct gccatttcct
     7261 ttaaatacaa tgaggtatta gatgttaagt attattttga cagggcatgg cgggtttgcc
     7321 agtggcatgg aaaaagcaat gaagcaaatc ctcggcgagc aatcgcagtt tattgccatc
     7381 gattttccgg aaacctcaag caccgcgctg cttacatcgc agcttgaaga ggccatcgcc
     7441 caactggatt gtgaagacgg cattgttttt ctgacggatt tgctgggcgg cacgccgttt
     7501 cgcgtagctt caacgctggc gatgcaaaaa ctgggctgtg aagtgattac cggcaccaat
     7561 ttgcaactgc tactggagat ggtgctggag cgcgaagggt taagcggtga agagtttcgt
     7621 gtgcaggcgc tggagtgtgg acatcgtggg ctgaccagtc tggtggacga gttaggccgc
     7681 tgccatgagg aatgtccggt cgaggaagga atatgacacc cgttttgcgc gccagaaggc
     7741 tgctgactga agagggatgg ctcgatgacc atcagttgcg tattgctgac ggtgtaatcg
     7801 cagaaatcga accgattcca gtgggcgtga ctgaacgcga tgcggaactg ctctgccccg
     7861 cttacatcga cacccatgta cacggtggtg cgggcgttga tgtaatggat gacgcgctgg
     7921 atgtgctcga caagctggca atgcacaaag cacgcgaagg tgtcggcagt tggttaccga
     7981 ccaccgtaac cgcgccgctt aataccattc atgcggcgct ggaacgtatt gctcaacgtt
     8041 gccaacgcgg cggacctggt gcacaaatac tggggagtta tctcgaagga ccgtacttca
     8101 cgccgcagaa taaaggcgcg catccgccgg agctgtttcg cgagctggaa attgccgagc
     8161 tggatcaatt gattgccgtt tctcagcaca ccttgcgcgt ggtggcgctg gcaccggaaa
     8221 aagaaggggc attgcaggcc atccgccatc ttaaacagca aaacgtaagg gtgatgctgg
     8281 ggcatagcgc ggcgacctgg caacaaaccc gcgccgcgtt tgatgctggt gccgacggcc
     8341 tggtgcattg ctataacggg atgacaggtt tacatcaccg cgaaccggga atggttggcg
     8401 cgggattaac ggacaagcgc gcctggctgg aactgatagc cgatggtcat catgtgcatc
     8461 cggcggcgat gtcgctgtgt tgttgctgtg cgaaagagag aatcgtgctg atcaccgacg
     8521 cgatgcaggc agccgggatg ccggatggtc gctatacgtt atgtggcgaa gaagtgcaga
     8581 tgcacggtgg cgttgtccgt accgcgtccg gtgggctggc gggcagtacg ctgtctgttg
     8641 atgcggcagt gcgcaacatg gttgagttgg cgggtgtaac gcctgcggaa gccatccata
     8701 tggcatcgct gcatccggcg cgtatgctgg gtgttgatga tgttctggga tcgcttaaac
     8761 cgggcaaacg cgccagcgtc gttgcgctgg atagcgggct gcatgtgcaa caaatcagga
     8821 ttcagggtca attagcttcg ttttgatggt ttgctccttt attgggcctt cacttccccc
     8881 gtaaggcctt tctttttctt tcgttttgat ctgtgcagcg gtgtcggatg cgacgctaat
     8941 gcgccttatc cgacctacgg ttggtgaccg taaggccgga taaagtattt acgccgcatc
     9001 cagcaatccc ttttacttcc cttgtctttt ctttcaacga tcacagattt cgttttattt
     9061 cctttttctc cattgaactt tcagtttctt ttctatagat tttaaccaac gaaagaaatc
     9121 accaagtgaa atgaaacgaa aggcaaacga aagcgacaac gcgacgtcaa gttcatcaga
     9181 ctaaggattg agttatgcca gaaaattaca cccctgctgc tgccgcaacc ggtacatgga
     9241 ctgaagaaga gatccgccat cagcctcgcg catggatccg ttcactcacc aacatcgacg
     9301 cgctacgttc cgcgctcaat aacttccttg aaccgttact gcgcaaagag aatctgcaaa
     9361 tcatcctgac cggagccgga acctcggcat ttatcggtga catcatcgcg ccgtggctcg
     9421 ccagtcatac cggtaaaaac ttcagcgccg taccgaccac cgatctggtc actaatccga
     9481 tggactacct gaatccagct catccgctgc tgttgatctc cttcggtcga tccggcaaca
     9541 gcccggaaag cgtcgccgcc gtggaactgg caaatcaatt tgtacccgag tgctatcacc
     9601 tgccgatcac ctgcaacgaa gcgggcgctc tttaccaaaa cgcgatcaac agcgacaacg
     9661 cgtttgccct actgatgcct gcagaaacgc acgaccgcgg ctttgcgatg accagcagca
     9721 ttaccaccat gatggtcagc tgcctcgcgg ttttcgcacc tgagacgatc aacagccaga
     9781 gcttccgcga cgtagcggat cgttgccagg cgatcctgac ctcactgggc gatttcagcg
     9841 aaggtgtgtt tggttactca ccgtggaaac ggatcgttta tctcggcagc ggtggcttac
     9901 agggcgcagc acgcgagtcg gcgctgaaag tactggaact gacggcgggt aaactggcgg
     9961 ccttttatga ttccccgacc ggattccgtc atggcccgaa atcgctggtc gataacgaaa
    10021 cgctggtggt ggtgtttgtc tccagccacc cttacacccg tcagtatgat cttgatctgc
    10081 tggcagaact ccgccgcgat aaccaggcat tgcgcgtaat cgccatcgcc gcggaaagca
    10141 acgacgttat taccgccggt ccacatatca tcctgccgcc gtcccgtcac tttatcgacg
    10201 ttgagcaggc attttgcttc ctgatgtacg cccagacatt tgcactgatg cagtcgctgc
    10261 acatgggtaa tacgccggat accccatcag ccagtggcac cgttaaccgc gtggtgcaag
    10321 gcgtaatcat tcatccgtgg caggcataag aggatcgcat tatgagcatt atctccacca
    10381 aatatctgtt acaggacgcc caggccaatg gctacgcggt gcctgctttt aacattcata
    10441 acgccgagac gatccaggcg atcctcgaag tgtgcagtga aatgcgatcg ccggtgatcc
    10501 tcgccgggac gccggggacc tttaaacata tcgcgctgga agagatctac gccctgtgta
    10561 gtgcctattc cacaacctac aacatgccac tggcgctgca tctcgaccac cacgaatcgt
    10621 tggatgatat tcgccgcaaa gtccacgcag gcgtgcgcag tgcgatgatc gacggcagcc
    10681 acttcccgtt tgacgagaac gtgaagctgg tgaaatcggt tgtcgacttc tgccactcac
    10741 aggattgcag cgtggaagca gaactgggcc gcctgggcgg tgttgaagat gacatgagcg
    10801 ttgacgccga aagtgcattc ctaaccgatc cacaagaagc taaacgcttt gtcgaactga
    10861 ctggcgtcga cagcctggcg gtagcgattg gtacggcgca cggcttatac agcaaaacgc
    10921 cgaagattga tttccagcgg ctggcggaaa ttcgtgaagt ggtggatgtt cctctggtgc
    10981 tgcatggtgc cagcgatgtc ccggatgaat ttgtccgtcg cactattgaa cttggcgtca
    11041 caaaagtgaa cgttgccaca gaattaaaaa tagccttcgc tggcgcggtt aaagcctggt
    11101 ttgcggaaaa tccacagggt aatgatcctc gttattatat gcgcgtcggt atggatgcga
    11161 tgaaagaagt tgtcagaaat aaaattaatg tctgtggttc agcgaatcga atttcagcat
    11221 aatcattagt tttttatgat ttatcccaat gtacttcccg attaatatcg gggagtgcct
    11281 taatggtaaa ggagataact aaaccttaat aa
//
LOCUS       DAPHCV010000132        11168 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.133, whole genome shotgun sequence.
ACCESSION   DAPHCV010000132 DAPHCV010000000
VERSION     DAPHCV010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.133"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            345..959
                     /gene="evgA"
                     /locus_tag="RH033_003958"
     CDS             345..959
                     /gene="evgA"
                     /locus_tag="RH033_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HDV2993072.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            964..4557
                     /gene="evgS"
                     /locus_tag="RH033_003959"
     CDS             964..4557
                     /gene="evgS"
                     /locus_tag="RH033_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HDV2993073.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADGDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRIRGINADYLSLLKRALNIKLTLRE
                     YADHQKAMDALEDGEVDIVLSHLVASPPLNDDIAATNPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFLLTRKDSIVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDETGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPAAIYSEDRENNVSFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVQNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     ESPFKDVFSNTHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASEHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FAAIAASKSIALSCSSTLPERYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTIFTITIPVEITQQVAAVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLNCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEITVFCQQND"
     gene            complement(4613..5758)
                     /gene="yfdE"
                     /locus_tag="RH033_003960"
     CDS             complement(4613..5758)
                     /gene="yfdE"
                     /locus_tag="RH033_003960"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HDV2993074.1"
                     /translation="MTNNECKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSVLCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5832..6776)
                     /gene="yfdV"
                     /locus_tag="RH033_003961"
     CDS             complement(5832..6776)
                     /gene="yfdV"
                     /locus_tag="RH033_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HDV2993075.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6846..8540)
                     /gene="oxc"
                     /locus_tag="RH033_003962"
     CDS             complement(6846..8540)
                     /gene="oxc"
                     /locus_tag="RH033_003962"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HDV2993076.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNGIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVASAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(8594..9844)
                     /gene="frc"
                     /locus_tag="RH033_003963"
     CDS             complement(8594..9844)
                     /gene="frc"
                     /locus_tag="RH033_003963"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HDV2993077.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(10357..10989)
                     /locus_tag="RH033_003964"
     CDS             complement(10357..10989)
                     /locus_tag="RH033_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HDV2993078.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT         3261 a         2521 c         2301 g         3085 t
ORIGIN      
        1 acagtgcatg tgttgcacat tatcccagga acgttggagg cctaatttaa atgaggtgca
       61 aaaaaaacaa cagaggacta aaccgtggct tttgcaatac aattcttacg cctgtaggat
      121 tagtgagaag acttatagtg ccaatgtgaa actataaatc atcggtacaa tacctgattt
      181 tattgttgac atttcatata tgccgactat ttatatggta tacttgtcga attatcttaa
      241 aggaagctca aattttctta tttttattga gaaaatgaga tgatgcctta tgtctgtatt
      301 actacaggga gaagggagat gcttcattgc aaagggaata atctatgaac gcaataatta
      361 ttgatgacca tcctcttgct atcgcagcaa ttcgtaattt attgatcaaa aacgatattg
      421 aaatcttagc ggagttgact gaagggggaa gtgccgttca gcgggtggaa acacttaaac
      481 ctgatatcgt catcattgat gtcgatatcc ccggagttaa cggtatccag gtgttagaaa
      541 cgctgaggaa gcgccaatat agcggaatta ttattatcgt ctccgctaaa aatgaccatt
      601 tttacgggaa acattgtgct gatgctggcg ctaatggttt cgtgagtaaa aaagaaggca
      661 tgaacaatat cattgcggct attgaagctg caaaaaatgg ctactgctat ttccccttct
      721 ctctcaaccg gtttgttgga agtttaacgt ccgaccagca aaaactcgat tccttatcga
      781 aacaagaaat tagtgtcatg cggtatattc ttgatggcaa ggataataat gacattgctg
      841 aaaaaatgtt catcagcaac aaaactgtca gcacttataa aagtcgcttg atggaaaaat
      901 tagaatgcaa atcactgatg gatctttaca cattcgcaca acgtaacaaa atcggctaac
      961 cacatgaagt ttttacccta tatttttctt ctctgttgtg gtctttggtc gaccataagt
     1021 ttcgcagacg gagattacat cgaatatcgt ggcatcagta gtaacaaccg tgtcacactt
     1081 gatccactac gtctgagcaa caaggaatta cgttggctag cgagcaaaaa aaatcttgtg
     1141 attgcagtac acaagtccca aacagctacg ttgttgcata ccgattcgca gcaacggatt
     1201 cgtggtatta atgctgatta tttaagtctt ttaaaaagag cgttaaatat caaattaaca
     1261 ctccgggaat acgcagatca tcaaaaagca atggacgcgc tggaagacgg tgaagttgat
     1321 atagtgttat cacatttagt tgcttcgccg cctcttaatg atgacattgc tgcaaccaat
     1381 cctctgataa ttacctttcc ggcgctggtc actacccttc acgattcaat gcgaccgctt
     1441 acctcatcaa aaccagtaaa tattgctcgg gtagcaaatt atcccccaga cgaggtaatt
     1501 catcaatcat ttccaaaagc aacaattatc tcttttacaa atttatatca ggcattagca
     1561 tccgtctcag ccggacagaa tgattacttt attggtagta acatcattac cagcagtatg
     1621 atttcccgtt attttactca ctccttaaat gtagtgaagt attataactc accgcgtcaa
     1681 tataactttt tattaaccag aaaagattct atcgttctta atgaagtact caatcgattt
     1741 gttgatgctt taacaaatga agttcgctat gaagtatcac aaaattggct tgatacagga
     1801 aacctggcct ttctgaacaa accattagaa ctcactgaac atgaaaaaca gtggattaag
     1861 cagcatcccg atttaaaggt gctagaaaat ccttactcgc caccctattc tatgacagat
     1921 gaaactggct cagttcgggg cgtgatgggt gatattctta atattattac cttgcaaaca
     1981 ggtttaaatt tttctccgat caccgtttca cacaatattc atgctgggac acagcttaac
     2041 cccggtggat gggatatatt gcccgccgct atttatagtg aagatcgaga aaataatgtt
     2101 tcatttgctg aagccttcat aacaacgcct tacgtttttg tcatgcaaaa agcgcctgac
     2161 agtgaacaaa cattaaaaaa aggaatgaaa gttgccattc cctattatta tgagcttcat
     2221 tcgcaattaa aagagatgta tccggaggta gagtggataa aagtcgataa cgccagcgct
     2281 gcatttcaca aggttaaaga aggtgaactt gatgctctgg tcgcgacaca gttaaattcg
     2341 cgttacatga tcgaccatta ctatcctaat gaactttatc attttcttat tcccggcgtt
     2401 cagaatgcat cactttcgtt cgcttttcct cgcggagaac cggaacttaa ggatattatt
     2461 aataaagcac tgaatgcaat tcccccaagc gaagttctgc gcctgaccga aaaatggatt
     2521 aaaatgccca atgtgaccat tgacacatgg gacctctata gcgagcaatt ttatattgtt
     2581 acgacattat ccgttttatt agttggcagt agccttttat ggggattcta cctgttacgc
     2641 tcagttcgtc gtcgtaaagt tattcagggt gatttagaaa accaaatatc attccggaaa
     2701 gcgctctcgg attccttacc gaatccaact tatgttgtaa actggcaagg taatgtcatt
     2761 agtcacaata gtgcatttga acattatttc actgctgatt actataaaaa tgcaatgtta
     2821 ccattagaaa acagtgaatc cccctttaaa gatgtttttt ctaatacgca tgaagtcaca
     2881 gcagaaacga aagaaaaccg aacaatatac acacaggtat ttgaaattga taatggcatc
     2941 gagaaaagat gcattaatca ctggcatacc ttatgtaatc tgccagcaag cgaacatgct
     3001 gtttatattt gtggttggca agatattacc gagacgcgtg atttaattca tgcactcgaa
     3061 gtagagagaa ataaagcgat caatgcaact gtcgcaaaaa gccagtttct ggcaacaatg
     3121 agtcacgaaa taagaacacc aataagctcc attatgggct tcctggaact tctgtcgggt
     3181 tctggtctta gcaaggaaca acgggtggag gcgatttcac ttgcctacgc caccggacaa
     3241 tcactcctcg gcttaattgg tgaaatcctt gatgtcgaca aaattgaatc gggtaactat
     3301 caacttcaac cacaatgggt cgatatccct actttagtcc agaacacttg tcactctttc
     3361 gctgcgattg ctgcaagcaa atcgatcgca ttaagttgca gcagtacgct tcctgaacgc
     3421 tacctggtta agatcgaccc tcaggcgttt aagcaggtct tatctaattt actgagtaat
     3481 gctctcaaat ttactaccga gggggcagta aaaattacga cctccctggg tcacattgat
     3541 gacaaccacg ctgttatcaa aatgacgatt atggattctg gaagtggatt atctcaggaa
     3601 gaacaacaac aactgtttaa acgctatagc caaacaagtg caggtcgtca gcaaacaggt
     3661 tctggtttag gcttaatgat ctgcaaagaa ttaattaaaa acatgcaggg cgatttgtca
     3721 ttagaaagtc atccaggcat aggaacgata tttacgatca caatcccggt agaaattacc
     3781 caacaagtgg cggctgtcga ggcaaaagca gaacaaccta tcacactacc tgaaaagttg
     3841 agcatattaa tcgcggatga tcatccgacc aacaggctat tactcaaacg ccagctaaat
     3901 ctattaggat atgatgttga tgaagccact gatggtgtgc aagcgctaca caaagtcagt
     3961 atgcaacatt atgatctgct tattactgac gttaatatgc cgaatatgga tggttttgag
     4021 ttgactcgca aactccgtga gcaaaattct tccttaccca tctgggggct tacagccaac
     4081 gcacaggcta acgaacgtga aaaagggtta aattgcggca tgaacttatg tttgttcaaa
     4141 ccgttgactc tggatgtact gaaaacacat ttaagtcagt tacaccaggt tgcgcatatt
     4201 gcacctcagt atcgccacct tgatatcgag gccctgaaga ataatacggc gaatgatcta
     4261 caactgatgc aggagattct catgactttc cagcatgaaa cgcataaaga tctacccgct
     4321 gcgtttcatg cactagaagc tggcgataac agaactttcc atcagtgtat tcatcgcatc
     4381 cacggtgcgg ctaacatcct gaatttgcaa aagttgatta atattagcca ccagttagaa
     4441 ataacacctg tttcagatga cagtaagcct gaaattcttc agttgctgaa ctctgtaaaa
     4501 gaacacattg cagagctgga ccaggagatt actgttttct gtcagcaaaa tgactaaata
     4561 gcggctccca caatgttcaa atgtgggagc tatttaccag cacatcttac atttatgatg
     4621 agaactcctg gcgaatttgt tccccatgct ggtcgagcgt tgccgctccc ggcataacat
     4681 gcgggtccgc gcagccgctg atttttatcg gattacccgg catcattatt cccccggctt
     4741 caatcaacat atttctcgcc tgagtttgtg gtaaattaat ggcctcagcc acacttaata
     4801 acggcgcgac gggcacacca acttcatgta ttctggctaa ccaaacttca gctgcctgcg
     4861 tttttaacgt tcgctcaata tattgtttaa gaatagcctg gttttgtacg cgtaaaatat
     4921 tgctgctaaa tcggggatca ttaaccagtt ctgtaagctc cagtgcctgg cataacacag
     4981 aaaaaagctt gtcattacca caacaaatcg taatcggctt atcctgagta tcgaaaacat
     5041 caaaaggtgc catgtaggga tggcgatttc ccaggcgttg tggtgacttc ccagtcgcga
     5101 tatatgccat cagcccatgc tccagaaaac tcagcgtggc atcaaacatc gctatatcga
     5161 catgcgcccc tctctggctc ttttcgcggc cataaagtgc actcactatt ccactgaata
     5221 aataaacacc gccacagaga tccgcaagag aggtaccaac gcgcactggc ggagcatcag
     5281 ggtatcctgt ttccatcatt atcccgctca ttgcctgaat gatggtatcg taggcaggag
     5341 catcttttag cggaccggta tgtccgaaac ctgacgatga agcatatata aggcgcgggt
     5401 tgatttcttg taacctttcc catgaaaacc cgagtttttc cattgtacct ggacgaaaat
     5461 tctcagctaa tacatcagct tgtttaagca tatttataaa tatactttta tcgtgatcat
     5521 tctttaaatc aagaaccaca ctctctttgc catgattaat aaaactgtaa tagagtgact
     5581 gtccatccac atagggacca aatgtgcggg tatcatcacc atgacccggc ggctcaactt
     5641 taattaccct tgcacccata ttacaaagaa gttgagttcc gaaaggccca ttaaggacat
     5701 gcgtcatatc gataactaat aagccttcaa acggcccttt gcattcatta tttgtcattt
     5761 gcttatgcct tgaaaaaagc cgaaggtgaa aagacacttc ggcatttatg taaaataaat
     5821 aacatgatta attatgaaac cagtcgactg acataaatcc acaaaggagc cgtgacgaca
     5881 aaacccagta cgctcacagc caatgacgct gtaccagtgc gggtgtagac attaaaccgg
     5941 ctggcaataa tgattccgga gaatgccggc ggtaatgcgc ctgccagtac catcatctgc
     6001 aaatgttcgc tattcaaatg acatgccata ccgacgagaa gcagcgccag tggcatcaga
     6061 atcagcttca ggaaagtgtt ataagcaatt tcggcactga actcgaattt atgtgcagcc
     6121 agagtcaacc ccgcagcgaa taccgctacc cctgagttag ccttcgcaat cagattaaag
     6181 gttgggtccc atgctgccgg aatttttacc ccaaccaaca ccaggatcgt tgccagaaca
     6241 ggtgcccata ctactggctc ctttgccgca gaaattaatg cgctcagatt actattcttc
     6301 ttaccatccg ctcctgagga aggattcagc aaatacagac caataggaat agtaattgcg
     6361 ttaacaataa tagaaataat tgctacaact aaacctgttg atacggaatc accataaata
     6421 ggatcgagaa ctgcaaatcc caggaatcca atggtaggtg aacctgcaat taatgcacat
     6481 actgctgctt ctgcatgagt acgtttaaaa aatttgtagc aaccgaacca ggagaaaaag
     6541 aaacatccga caataaccac aagtgataca agggtcagac gagtgtccgc aaaaatcatt
     6601 tccctgtttg cccgggtaat agatacaaat agagctgcag gaagagcgta gtttaatacc
     6661 agtttattaa atgcccgggc ttgatcttct gaaaaagttt ctcgtctgcc gctaaaataa
     6721 cccaacagca tgattacgat aataggcaat aaatcgccaa taaaaaatgt tagcataata
     6781 gtatttccga taagggtccc ctcaccatcg cataataagt tggcaagagg agacgatgtc
     6841 agtatttaat taccagcgac ttgttttggg ttaagtttgg taatatggcc actttcagtt
     6901 cctgctgctg ggtcgatgac cacattaata atggtcggtt tgcgcgactg aattccggtg
     6961 gttaaagcat gacgaagttc atctgtcgtg gtgacgttat agccaacgcc acgaaacgca
     7021 tccattaatt tgtcatacct tgcatggtgc aacagatccg ttggcgatgg tgcaccagcg
     7081 ccgctgagat caacaccgtc tcctctgtag atgccgccat tattaaaaat aacgatcgtc
     7141 accggcaggt tatatcgaca aatcgtttca atttccatcc cactgaaacc aaaagcacta
     7201 tcaccttcaa tggcgacaac cggagaacca gaggtcacgc tagcaccgat ggcatagccc
     7261 ataccgatgc ccatgacacc ccaggtgcca caatccagac gacgacgtgg tttatacata
     7321 tcaataatat ttcgtgcatt atccagggta tttgcccctt cattaactaa ataaatatcc
     7381 tggttctcgc gcaaaacatc gcgcacggca ctcaatgcat taaagtaatt taatggttgg
     7441 gtatctgtac ttaacttttc atgcattttt tgtgcatttt gctgcttgtg gatatttaaa
     7501 atatcgcgcc acaccagtgg agtcgtgaat gtgttttgtt tcaattctgc cagcatacct
     7561 tgcatactgg atgcaatatc gccaacgact ggcacagcaa tggggcggtt gctgtcaatt
     7621 tcctgcggtt caatatccag ttgaataaac tgtgtatctg ccgcccatcc ttttttaccg
     7681 tgtgccagta accaattcag tcgtgcgcca acaagcatga caacgtcagc atttgccagg
     7741 gcaaacgaac gcgcagctgc cgcagaaagt ggatgcgtat cttcgaggat ccctttcgcc
     7801 atagacattg gcaggaatgg aatctgggca ctttcaataa attcacgaag ctgttcatca
     7861 gcttgtgaat acgccgcgcc tttgccaagg ataattaatg gtcgttcagc ttttgctaaa
     7921 agcgaaattg cgctagcgac tgacttcggg catggcaata atgctggcga cggattttca
     7981 acttttacaa tcgtggttaa cgcttcgtct ttttccatcg tcgcggccag gacatttgct
     8041 ggcaaatcaa gataaactcc gccagggcga cccgatactg agacccggat agcgcgtgcc
     8101 aatgcaatgc caagatcctg cggctgatta acgcggaatg ctgctttggc atacggtttt
     8161 gccgcattca tttggtccag ctcttcataa tcaccttgct gtaggtcgac gatcgcgcgg
     8221 tcgctggagc cgctaatcat gatcatcgga aaaccattta ccgttgcgtt ggccaatgcg
     8281 gtcaaaccat tgaggaatcc tggcgcagaa actgtcaggc agatccccgg tttttgggta
     8341 agaaaaccgc ttgccgcagc ggcatagcct gccgactgct catgacgaaa accaatataa
     8401 cgaatgcctt ccgcctgggc atggcgcgcc atatccgtca caggaatacc tacaacacca
     8461 taaatagtgt caataccatt ctgttttaat gcttcaacga tgatatgcat accatctgtc
     8521 atttgaagtt gatctgacat gattaataac ctcgaaattt ttataaattg ggagccagaa
     8581 gcccccgtta atatcagatg gcgtggtttt gcttcattgc agcaatttca tcgtcgctat
     8641 aacccagctc ctgcaataca gcagcggtat gctcacctaa tagcggcgca gctttaatat
     8701 ccggcgtaaa ggcagagaat ttcattggac aaccaacggt cagatatttt ccacgcaacg
     8761 gttgttccac ttcgacaaca ctaccacttt ggcgcaaaga gggatcaagt gaaatttctt
     8821 tcatacttaa aaccggtgca caaggaatat caaactgagt caaataggcc accgcttcat
     8881 gtttatcaat agtgacagtg tatttttcga tttcagcaaa aatatcgaaa atatgtggct
     8941 gtcgggcatg ggctgtactg tatgccggat cggtaatcca ttctggtttg ccgatggctt
     9001 tacaggtgtt ttcccagttt tgctcctgaa tagtgaaata aatataggcg ttaggatcgg
     9061 tttcccagcc tttacatttc aggatccagc caggctgacc gccaccaccc gcattaccac
     9121 cgcggggaac tgcatcacca aatgtgccat tcggatactg cgggtattct tccagatacc
     9181 ccaacttatc gagacgctgc tggtcgcgta atttcacgcg gcaaaggttc aacacggcat
     9241 cctgcattga catggtgact cgttgcccac gccccgtttt ttcgcgatgc agcaaagcag
     9301 caagtaaacc gatcagcaaa tgcattccgg tgttgctgtc acccaacgct gcagcgctta
     9361 ccagcggcgg accgtcccaa aaacctgtag tagatgccgc gccacccgct gcctgagcaa
     9421 cgttttcata ggcttttaca ttcacataag gcgaacactc atcaaaccct ttgatcgaac
     9481 caaaaatcag acgtggattg atttcttgaa tatgctccca ggtgaagccc atatgatcaa
     9541 tggcccctgg atgaaagttc tcgactaaga tatcagcttc gcggatcagc ttttccatta
     9601 cctctttgcc ttccgctgtt ttggtattta attcaataga acgtttgtta ctgttaagca
     9661 tggtgaagta aagcgcatcg atatcaggaa tatctcgcag ctggtggcgc gttacgtcac
     9721 caacgccggg acgttcaatt ttaatgacgt cagcgccaaa ccaggccagc atctgagtac
     9781 aagatgggcc agattgcaca ccagtgaaat cgagaacttt aattccttga agtggagttg
     9841 acatagcaat acctattagt taattaaata tatgttaatt tagcgtttat ttaaggcata
     9901 atgctctcta taaataatcc gcaaaatatc gtcgtagctg cactcgatat ttttaaattc
     9961 ctgtatcaag tgctgataat catttcatcg ccacttccaa ttcctttctg catggtaagt
    10021 agtgaggacg aaaagaagaa tatgataaat attggttcgc ggtaatcaga gagggccgcg
    10081 acatttgtga tgttattctt acaactgtaa gaagcgccgg tgatgtacaa gtaagggtat
    10141 gattacgctt tttaaagcgt accataaaac ataacataat aaatgattta tgatgacacg
    10201 ggattattgc ggtttacttt gaagaatata aaatgcgagt ttatagcacc gcgagcgcca
    10261 aataatttta tggccacaac ctgtcgtatt gtggccataa aaaattgtcg gctggaggga
    10321 gtaaaatcct ttacctcaat atattgcctg tcagctttag ttcacaaaca gtgcttcgct
    10381 atcgacgatg attccatctt ccgcaccttt aagcgccagg tttgcctcgt agtattgctt
    10441 tttatcaagc agtttctgcg catcagcaag tgcattacga gtctgtttga gcggcatcag
    10501 atattggttt tccattacac caacaccggc caggcgaagc tcttccattg cgcccttttt
    10561 atcccctttc gccatttttt cgttggcaat ttttatcgcg gcttcttttt cgggtgtcgc
    10621 tacataactc tcagaaattc cgacagatgc attaatgaca atatattggt catcattgag
    10681 attggtcttt ttacctggct tagcgaactt tgcccattcg gtactgtcat ctgacaacaa
    10741 agctgaagct tcattggtca gttcttttgc cttttccgga tcgccatgaa acagagccag
    10801 gcgcgctacc tgcacgtcac gcatcgcata taaaccttgt tcagagatac gctctgctgc
    10861 tgccgttttc tgggtttgct gaacttgctg agtttgctgt tgtgccgcta ccggtgcatt
    10921 atcagccgcc catacagttg aagatgccag aattgctgtg accatcgtag ccataattaa
    10981 acgtttcata cttacctcat tctttaacag ttttagaaaa cgccgccatc atattgggtg
    11041 agcatgaaat gagaaatcag agagcaacgg tcattttgaa agggttatct tacagttgta
    11101 gttattatcg caagaagtat tttagtctta caattgtagg aatatgcttc ctgtcacaga
    11161 gaactatt
//
LOCUS       DAPHCV010000133        11064 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.134, whole genome shotgun sequence.
ACCESSION   DAPHCV010000133 DAPHCV010000000
VERSION     DAPHCV010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.134"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            227..1369
                     /locus_tag="RH033_003965"
     CDS             227..1369
                     /locus_tag="RH033_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV2993079.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGKDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPSAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEIQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1376..2140)
                     /gene="glcC"
                     /locus_tag="RH033_003966"
     CDS             complement(1376..2140)
                     /gene="glcC"
                     /locus_tag="RH033_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HDV2993080.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDASPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMKQG"
     gene            2391..3890
                     /gene="glcD"
                     /locus_tag="RH033_003967"
     CDS             2391..3890
                     /gene="glcD"
                     /locus_tag="RH033_003967"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HDV2993081.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVSGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVQNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLNAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3890..4942
                     /gene="glcE"
                     /locus_tag="RH033_003968"
     CDS             3890..4942
                     /gene="glcE"
                     /locus_tag="RH033_003968"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HDV2993082.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRATLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGGGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMIDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLPAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4953..6176
                     /gene="glcF"
                     /locus_tag="RH033_003969"
     CDS             4953..6176
                     /gene="glcF"
                     /locus_tag="RH033_003969"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HDV2993083.1"
                     /translation="MQTRLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVCYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMPANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLRKEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLFRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            6181..6585
                     /locus_tag="RH033_003970"
     CDS             6181..6585
                     /locus_tag="RH033_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HDV2993084.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6607..8778
                     /gene="glcB"
                     /locus_tag="RH033_003971"
     CDS             6607..8778
                     /gene="glcB"
                     /locus_tag="RH033_003971"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HDV2993085.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGVELDAAAFWRNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPDHVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDTAALTCILLKNNGLHIELQIDANGRIGKDDSAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFSRVETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKDQVQASLENMAKVVDQQNAGDPAYRPMVE
                     NFANSCAFKAACDLIFLGVKQPNGYTEPLLHAWRLREKENH"
     gene            9134..10816
                     /gene="glcA"
                     /locus_tag="RH033_003972"
     CDS             9134..10816
                     /gene="glcA"
                     /locus_tag="RH033_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HDV2993086.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
BASE COUNT         2586 a         2756 c         3084 g         2638 t
ORIGIN      
        1 tattgtgaat gatatttaat ttatgaatat attaagaaga agccttgtcc ggtcgcctta
       61 aatcgacaaa aggcattgtg gcgcttctgt gtagtctctg accttatcgt aatcattaat
      121 aatactattt attcgtcgtt tgtattcatt gggttgattg ttaaggtgcc gttcgttgct
      181 gttgcaacct gtttgtcacc gttaattatt tgttacccac agaactatgg aatgcgatct
      241 gctgatgatt aaaattgaaa aagtaattaa taaaaatgac cttaaggcat ttatcgcttt
      301 tccgtcatca ctttatcccg acgatccaaa ctggataccc cctttattca ttgaacgcaa
      361 cgagcatttg tctgcgaaaa atccggggac ggaccatatc atctggcagg cgtgggtggc
      421 aaaaaaagcg gggcagatag ttgggcgtat taccgcgcaa atcgatacct tgcaccgcga
      481 gcgttacggc aaagataccg gtcatttcgg catgattgac gccattgatg atccgcaggt
      541 ttttgccgcg ttgtttggcg cagcggaagc gtggttgaag tcacaaggtg caagtaagat
      601 cagcggtcct ttcagcctga atatcaatca ggaaagcgga ttactgattg aaggtttcga
      661 tacaccaccc agtgcgatga tgccacacgg taaaccgtgg tatgccgcgc atattgaaca
      721 actgggttat cacaaaggca ttgatttact ggcgtggtgg atgcagcgaa ccgatctcac
      781 tttctctccg gcgctaaaaa aactgatgga tcaggtgcgc aaaaaagtga ccattcgctg
      841 catcaatcgc cagcggtttg ccgaagagat acagatcctg cgtgagattt tcaattctgg
      901 ctggcagcac aactggggat ttgtgccgtt taccgaacat gaattcgcga ccatgggcga
      961 tcaacttaaa tatctggtgc cggacgatat gatctacatc gctgagattg attctgcacc
     1021 ctgcgcgttt attgttggct tgccgaacat caacgaggcg attgccgatc tgaacggatc
     1081 gctctttccc ttcggctggg caaaattgct gtggcgtttg aaagtcagcg gtgtgcgaac
     1141 tgcgcgagtg ccgctgatgg gcgtgcggga cgagtatcag ttcagccgca tcggcccggt
     1201 aattgcgctg ttattgattg aagccttacg cgatccgttt gcccgtcgga agatcgatgc
     1261 gctggagatg tcatggatcc tcgaaaccaa tacaggcatg aataacatgc tggaacgcat
     1321 tggggcggag ccgtacaagc gttaccgatt gtatgaaaag cagatttgat ggcagtcacc
     1381 cctgcttcat ctccagcggc accgccgagc gaatcaaatg gtggccttcc agctcgatat
     1441 cgtggagatt ctttttcacg gtccgcacat gatcacgtgc tgcgcgctgg gcgacgtgcg
     1501 gcagccgctg caacaccgcg ttgtagatcc gcgcatgctg gcgatcgatc tgcttttttt
     1561 gctgtggtcg atggtagaga ttatttacgc tggcaaacac tgaattaaac atcagatcgg
     1621 tcaatgattg cagcgtaaac accagcacct gattgtgaga agcctgacaa atggcgagat
     1681 ggaaagcgtg atccaactgc gcatgttcga tcagcgaaat ctctttgttg ttctcactgg
     1741 cggcgagcat tttttcataa cagcgggtta tcacaacaaa atcagcctgg gttcccagcg
     1801 ttgccgccag ccttgccgat tcgccctcca gtaatgcgcg aacgtcgagc agatcgtaca
     1861 gcgttcgcgg ctgcgtactg aacagatgga tcagcgggct ggcgtcctgc acccgattaa
     1921 gccgtgcgac acgagaatcg cgaccctgcg ccgtttcaat aatcccgcgc ccgcgcagca
     1981 cggtcagccc ttcacgcagt gcggagcgtg agaagccgag cttttcacac agtcgacgtt
     2041 ccgagggaag cggctgaccg accttcaaca cgccgtcgat aattaaccgt tcgatactct
     2101 ctgcaaccac ttcgcaaata gggcgacgtt catctttcat tcccggacct cgtgcacagg
     2161 taggaccaat ttttctgttc ttaactcgca aaacacgcac atcacgcaag tgattatgtt
     2221 acatcaattt aacattaagt taaccaaggc aaggtcacag agctggaaaa aaaatggtct
     2281 gaccggtagc taaagagata gacgaaaacg aaaagcccgc ttaataactg ttcacagaag
     2341 cagcgcgcaa aaatcagctg ccacacaaca caacaaagcg aagcctattc atgagcatct
     2401 tgtacgaaga gcgtcttgat ggcgctttac ccgatgtcga ccgcacatcg gtactgatgg
     2461 cactgcgtga gcatgtctct ggacttgaga tcctgcatac cgatgaggag atcattcctt
     2521 acgagtgtga cgggttgagc gcgtatcgca cgcgtccatt actggttgtt ttgccaaagc
     2581 agatggaaca ggtgacggcg attctggctg tctgccaccg cctgcgtgta ccggtcgtga
     2641 cccgtggtgc aggcaccggg ctttctggtg gcgcgctgcc tctggaaaaa ggtgtgttgt
     2701 tggtgatggc gcgctttaaa gagatcctcg acattaaccc cgttggtcgc cgcgcgcgcg
     2761 tgcagccggg cgtgcgtaac ctggcgatct cccaggccgt tgcgccgcat aatctctatt
     2821 acgcgccgga tccttcctca caaatcgcct gttcaattgg cggcaatgtg gcggaaaatg
     2881 ccggtggcgt tcactgcctg aaatatggtc tgaccgtaca gaacctgctg aaaattgaag
     2941 tgcaaacgct ggacggcgag gcgctgacgc tgggatcgga tgcgctggat tcgcctggtt
     3001 ttgacctgct ggcgctgttc accggatcgg aaggtatgct cggcgtgacc accgaagtga
     3061 cggtaaaact gctgccgaag ccgcccgtgg cgcgggttct gttagccagc tttgactcgg
     3121 tagaaaaagc cggacttgcg gttggtgaca tcatcgccaa tggcattatc cccggcgggc
     3181 tggagatgat ggataacctg tcgattcgcg cggcggaaga ttttattcat gccggttatc
     3241 ccgtcgatgc cgaagcgatt ttgttatgcg aactggacgg cgtggagtct gacgtacagg
     3301 aagactgcga gcgagttaac gacatcttgt tgaacgcggg tgcgactgac gtccgtctgg
     3361 cacaggacga agcagagcga gtacgtttct gggccggtcg caaaaatgcg ttcccggcgg
     3421 taggacgtat ctccccggat tactactgca tggatggcac catcccgcgc cgcgccctgc
     3481 ctggcgtact ggaaggcatt gcccgtttat cgcagcaata tgatttacgc gttgccaacg
     3541 tctttcatgc cggagacggc aacatgcacc cgttaatcct tttcgatgcc aacgaacccg
     3601 gtgaatttgc ccgcgcggaa gaactgggcg ggaagatcct cgaactctgc gttgaagttg
     3661 gcggcagcat cagtggggaa catggcatcg ggcgcgaaaa aatcaatcaa atgtgcgccc
     3721 agttcaacag cgatgaaatc acgaccttcc atgcggtcaa ggcggcgttt gaccccgatg
     3781 gtttgctgaa cccaggaaaa aacattccca cgctacaccg ctgtgctgaa tttggtgcca
     3841 tgcatgtgca tcacggtcat ttaccttttc ctgaactgga gcgtttctga tgctacgcga
     3901 gtgtgattac agccaggcgc tgctggagca ggtgaatcag gcgattagcg ataaaacgcc
     3961 gctggtgatt cagggcagca atagcaaagc ctttttaggt cgccctgtca ccgggcaaac
     4021 gctggatgtg cgttgtcatc gcggcattgt taattacgac ccgaccgagc tggtgataac
     4081 cgcgcgtgcc ggaacgccgc tggtggcgat tgaagcagcg ctggagagcg ccgggcaaat
     4141 gctcccctgc gagccgccgc attatggtga agaagccacc tggggcggga tggtcgcttg
     4201 cgggctggcg gggccgcgtc gcccgtggag tggttcggtc cgcgattttg tcctcggcac
     4261 gcgcatcatt accggcactg gaaaacatct gcgttttggt ggcgaagtga tgaaaaacgt
     4321 tgccggatac gatctctcac ggttaatggc cggaagctac ggttgtcttg gcgtgctcac
     4381 tgaaatctcg atgaaagtgt taccgcgacc acgcgccacc ctgagtctgc gtcgggaaat
     4441 cagcctgcaa gaagccatga atgaaatcgc ccagtggcaa ctccagccat tacccattag
     4501 tggcttatgt tacttcgaca atgcgttgtg gatccgcctt gagggcggcg gtggatcggt
     4561 aaaagcagcg cgtgaactgc tggggggcga agaggttgcg ggtcagttct ggcagcaatt
     4621 gcgtgaacaa caactgccgt tcttctcgtt accaggtacc ttatggcgca tttccttacc
     4681 cagcgatgcg ccgatgatag atttacccgg cgagcaactg atcgactggg gcggggcgct
     4741 acgctggctg aaatcgacag ccgatgacaa tcaaatccat cgcatcgccc gcaacgctgg
     4801 cggtcatgcg acccgcttta gtgccggaga tggtggtttt gccccgctac cggctccttt
     4861 attccgctat caccagcagc ttaaacagca gctcgaccct tgcggcgtgt ttaaccccgg
     4921 tcgcatgtac gcggaacttt gaggagcagg ctatgcaaac ccgattaaca gaagagatgc
     4981 ggcagaacgc gcgcgcgctg gaagccgaca gcatcctgcg cgcctgtgtt cactgcggat
     5041 tttgtaccgc aacctgccca acctatcagc ttctgggcga tgaactggac gggccgcgcg
     5101 ggcgcatcta tctgattaaa caggtactgg aaggcaacga agtcacgctt aaaacacagg
     5161 agcatctcga tcgctgtctc acttgccgca attgtgaaac cacctgtcct tctggtgtgt
     5221 gttatcacaa tttgctggat atcgggcgtg atattgtcga gcagaaagtg aaacgcccac
     5281 tgccggagcg aatgctgcgc gaaggattgc gccaggtagt gccgcgtccg gcggtcttcc
     5341 gtgcgctgac gcaggtaggg ctggtgctgc gaccgttttt accggaacag gtcagagcaa
     5401 aactgcctgc tgaaaccgtg aaagctaaac cgcgtccacc gctgcgccat aagcgtcggg
     5461 ttttaatgct ggaaggctgc gcccagccta cgctttcgcc caacaccaac gcggcaactg
     5521 cgcgagtgct ggatcgtctg gggatcagcg tcatgccagc taacgaagca ggctgttgtg
     5581 gcgcggtgga ctatcatctt aatgcgcaag agaaagggtt agcgcgggcg cgcaataata
     5641 ttgatgcctg gtggcccgcg attgaagcag gtgccgaggc aattttgcaa accgccagcg
     5701 gttgcggcgc gttcgttaaa gagtatgggc agatgctgaa aaacgatgcg ttgtatgccg
     5761 ataaagcgcg tcaggtcagt gagctggcgg tcgatttagt cgaacttctg cgcaaggaac
     5821 cactggaaaa actggcaatt cgcggcgata aaaaactggc cttccactgc ccgtgtaccc
     5881 tacaacatgc gcaaaagctg aacggcgaag tggaaaaagt gttatttcgt cttggattta
     5941 ccttaacgga cgttcccgac agccatctgt gctgcggttc agcgggaaca tatgcgttaa
     6001 cgcatcccga tctggcacgc cagctgcggg ataacaaaat gaatgcgctg gaaagcggca
     6061 aaccggaaat gatcgtcacc gccaacattg gttgccagac gcatctggcg agcgccggtc
     6121 gtacctctgt gcgtcactgg attgaaattg tagaacaagc ccttgaaaag gaataacaaa
     6181 atgaaaacta aagtcattct tagccagcaa atggcgagtg caattattgc cgcaggtcag
     6241 gaagaggcgc agaaaaacaa ctggtctgtt tccattgctg ttgccgatga cggcggtcat
     6301 ctgctggcgt taagtcgcat ggacgattgc gcgccgattg cggcttacat ctcccaggag
     6361 aaagcgcgta ccgccgcgct ggggcgtcgt gaaactaagg gctatgaaga gatggtgaac
     6421 aacggacgta ccgcgttcgt gactgcgccg ttactgacgt cgctggaagg cggcgtaccg
     6481 gttgttgttg atgggcaaat tattggtgcc gtgggcgttt ctggtttaac cggagcacag
     6541 gatgcgcagg tcgcgaaagc ggcagcagcg gtgttggcga aataagcgaa aacgaggaga
     6601 gaaacaatga gtcaaaccat aacccagggc cgtttacgca ttgacgccaa ttttaaacgt
     6661 tttgtggatg aagaagtttt gccgggcgtg gaactggacg ctgccgcgtt ctggcgcaat
     6721 gttgatgaga tcgttcacga tctggcgcca gagaatcgtc agttgctggc agagcgtgat
     6781 cgcattcagg cggcgcttga tgagtggcat cgcagcaatc cggggccggt aaaagataaa
     6841 gcggcctata aatctttcct gcgtgaactg ggctacctgg tgccacagcc tgaccacgtt
     6901 acggtggaaa ccacgggcat cgacagcgaa atcaccagtc aggcggggcc acagctggtg
     6961 gttccggcga tgaacgcccg ctacgcgctg aacgcggcga acgctcgctg gggctcactg
     7021 tacgatgcgt tatacggcag tgacatcatc ccgcaggaag gggcgatggt cagcggctac
     7081 gatccgcagc gtggtgagca ggttatcgcc tgggttcggc gtttcctcga tgaatcacta
     7141 ccgctggaaa acggcagcta tcaggatgtg gtggcgttta aggtggtcga taaacaatta
     7201 cgcatccagt tgaaaaatgg taaagaaacc acgttacgta ctccagcaca gtttgtcggt
     7261 taccgtggcg atactgctgc gctgacctgc attttgctga aaaataacgg cctgcatatt
     7321 gagctacaaa tcgatgccaa cgggcggatt ggcaaagacg attcggcgca catcaacgat
     7381 gttatcgtcg aagcggccat cagtaccatt ctcgactgcg aagattcggt tgcggcggtt
     7441 gatgcggaag ataaaatcct gctgtaccgc aacctgctgg gcctgatgca ggggactctg
     7501 caagagaaaa tggagaagaa cggtcggcaa atcgtgcgta aactgaatga cgatcgtcag
     7561 tacactgccg ccgatggatc tgaaatttct ctgcacggac gctcgttgct gtttatccgc
     7621 aacgtgggtc atttgatgac cattcctgtg atttgggaca gcgaaggcaa tgaaatcccg
     7681 gaaggcattc ttgatggcgt catgactggc gcgattgccc tctatgattt aaaagtgcag
     7741 aaaaactcgc gcactggcag cgtctatatt gtgaaaccga aaatgcacgg tccgcaggaa
     7801 gtggcgttcg ccaacaaact gttttcccgc gttgagacaa tgctcggtat ggcaccgaat
     7861 accctgaaaa tgggcattat ggatgaagaa cgccggacct cgctgaactt gcgtagctgt
     7921 atcgctcagg cgcgcaaccg cgtggcgttc atcaataccg gtttcctcga ccgtaccggc
     7981 gatgaaatgc attcggtgat ggaagcgggc ccgatgctgc gtaaaaacca gatgaaatcg
     8041 acgccgtgga tcaaagccta cgaacgtaat aacgtgcttt ccggtctgtt ctgtgggcta
     8101 cgtggaaaag cgcaaatcgg taaaggcatg tgggcaatgc cggacctgat ggcagacatg
     8161 tacagccaga agggcgacca attgcgtgcc ggggcaaaca cggcctgggt tccgtcgcca
     8221 actgccgcta cgctccatgc gctacactac caccaaacca acgtacagag cgtgcaagcc
     8281 aacattgccc agaccgagtt caatgctgaa tttgaaccgc tgctggacga tctgctgact
     8341 attccggttg ctgaaaacgc taattggtcg gcgcaagaga tccaacaaga gctggataac
     8401 aacgtgcagg ggattctggg gtacgtggtg cgttgggtgg agcaggggat tggttgttca
     8461 aaagtgccgg atattcacaa cgtggcgttg atggaagacc gcgcaacgct gcgtatttcc
     8521 agccagcata tcgccaactg gttacgtcac ggtattctga ccaaagatca ggtgcaggcg
     8581 tcgctggaga atatggcgaa agtggttgat cagcaaaacg ctggcgatcc ggcttatcgt
     8641 ccaatggtgg agaatttcgc taactcgtgt gcttttaaag ctgcctgcga tttgatcttc
     8701 ctcggagtga aacagccaaa cggctatacc gaaccgttat tacacgcatg gcgtttacgc
     8761 gaaaaagaaa atcattaata gcgtcaaaaa aagccccggc aagatttatt gtcggggcta
     8821 tgatagtaac tgcggtagta catttatccc ggaaataacc ggagcgatta tcaggggtta
     8881 acaatattaa atacccgtag cgcatcacta gcgggattaa aacaacaata tggacatctt
     8941 aacgatactc tgcgcctgcg tgtgcggaac agaaaccctt acccgatata cttgttatgc
     9001 cagaggtagc aattgcgtta gcggcgtact gttgctgtaa agtataacgc ctatatttta
     9061 cagttcagtc ctcttttgct tattacaacc ttaagtatat caagcatata aagataataa
     9121 gagactgaac aatatggtta cctggaccca aatgtatatg ccgatgggag gactggggct
     9181 atccgctctg gtcgccctga tcccaataat attcttcttc gttgcactcg cggtattacg
     9241 tctgaaagga catgtcgctg gagcaataac ccttatatta tctattctga ttgcgatatt
     9301 cgcctttaaa atgccgattg atatggcatt tgctgctgcg ggctatggct ttatttatgg
     9361 tttatggcct attgcgtgga ttattgtcgc ggcggtattc ctgtataaat taaccgttgc
     9421 cagcggccag ttcgatatta tccgcagctc ggttatctcc atcaccgacg atcagcgttt
     9481 gcaggtgtta ctgattggtt tctcctttgg tgcgctgctg gaaggggcgg ctggctttgg
     9541 cgcgccggtg gcgattaccg gtgcgctgct ggtgggcctg ggcttcaaac cgttatacgc
     9601 ggcggggctg tgtctgattg ccaatactgc gccggtggcg tttggtgcgt tgggcgtgcc
     9661 gattctggtc gccggtcagg taacgggaat cgatccgttc cacattggcg caatggcggg
     9721 acgtcagtta ccgttcctgt cggttcttgt accgttctgg ctggtagcaa tgatggacgg
     9781 ctggaaaggg gtgaaagaga cgtggccagc ggcgctggtt gctgggggaa gcttcgctgt
     9841 cacgcagttc tttacctcta actatattgg tccggaactg ccggatatta cttcggcgct
     9901 ggtgagtatc gtctcactcg ctttattcct taaagtctgg cggccgaaaa ataccgaaac
     9961 ggcaatcagc atgggacaat ccgcaggagc gatggtggtg aataaaccat cttctggcgg
    10021 tcccgtgcct tcagaatata gtctggggca aatcattcgt gcgtggtcac cgtttttaat
    10081 cttaacggtg ctggtcacca tctggaccat gaagccgttt aaagcgttat ttgctccggg
    10141 cggcgcgttt tattcactgg tgattaattt ccagatccct catttgcatc aacaagtgtt
    10201 gaaagcggca cccattgtcg cccaaccaac gccaatggat gcggtgttta aattcgaccc
    10261 tctctcggct ggcggcaccg ctatttttat tgcggcgatt atctctatct tcatcctcgg
    10321 cgtgggcatc aagaaaggga ttggcgtctt tgccgaaacg ctaattagtc tgaagtggcc
    10381 gatattgtcg attggcatgg tgctggcgtt cgccttcgtc accaactatt ccggtatgtc
    10441 caccacgctg gcgctggtac tggcaggtac aggcgtgatg ttcccgttct tttcgccatt
    10501 cctcggctgg ctgggggtat tccttaccgg ctcggacacc tcctctaacg ccctgtttgg
    10561 ttcactgcaa tcgaccacgg cgcagcaaat caacgtctct gacacactgc tggtggcagc
    10621 aaataccagc ggcggcgtaa ctggcaagat gatctccccg caatctatcg ccgtggcctg
    10681 cgccgcgacg ggcatggtgg gccgggaatc tgaactgttc cgctacaccg tgaagcacag
    10741 tctgattttt gccagcgtta tcggcattat cacgctgttg caggcgtatg tgtttactgg
    10801 gatgttagtc tcgtaaatca ataatgccgg atgatcgtta atatcatccg gcttcattta
    10861 cattattctg tcttattaat tgaaaattat ttctcgcttt attatccatg cggcaataat
    10921 attttgtgtt tagataacct gctaaataat taagatcgtg agaacatcta ttttttatca
    10981 cgcaaaacaa gtctgtacta attatgttcg cttgatgagc aaacaccgtt ctatctgttg
    11041 cttttgcaat cttaatcgtt aaaa
//
LOCUS       DAPHCV010000134        10888 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.135, whole genome shotgun sequence.
ACCESSION   DAPHCV010000134 DAPHCV010000000
VERSION     DAPHCV010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.135"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            81..842
                     /gene="adiY"
                     /locus_tag="RH033_003973"
     CDS             81..842
                     /gene="adiY"
                     /locus_tag="RH033_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HDV2993087.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            979..2316
                     /gene="adiC"
                     /locus_tag="RH033_003974"
     CDS             979..2316
                     /gene="adiC"
                     /locus_tag="RH033_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HDV2993088.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSCLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2420..4063
                     /gene="eptA"
                     /locus_tag="RH033_003975"
     CDS             2420..4063
                     /gene="eptA"
                     /locus_tag="RH033_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HDV2993089.1"
                     /translation="MLKRLLKRPSLNLFSWLLLATFYISVCLNIAFFKQVLQVLPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLGSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMITNIIDTTPAESYALMTPRMLLTLGLSGVLAAIIACWIKIKPTTSRLRSVLFRGAN
                     ILISVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRSANLPLIR
                     IGEDAHRNPLMQNGKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEDYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4060..4728
                     /gene="basR"
                     /locus_tag="RH033_003976"
     CDS             4060..4728
                     /gene="basR"
                     /locus_tag="RH033_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HDV2993090.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4729..5829
                     /gene="pmrB"
                     /locus_tag="RH033_003977"
     CDS             4729..5829
                     /gene="pmrB"
                     /locus_tag="RH033_003977"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HDV2993091.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVVNQI"
     gene            complement(5805..5894)
                     /gene="pmrR"
                     /locus_tag="RH033_003978"
     CDS             complement(5805..5894)
                     /gene="pmrR"
                     /locus_tag="RH033_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HDV2993092.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFTTY"
     gene            complement(6006..7508)
                     /gene="proP"
                     /locus_tag="RH033_003979"
     CDS             complement(6006..7508)
                     /gene="proP"
                     /locus_tag="RH033_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HDV2993093.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7772..8650)
                     /locus_tag="RH033_003980"
     CDS             complement(7772..8650)
                     /locus_tag="RH033_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HDV2993094.1"
                     /translation="MTKTLLDGPGRVLESIHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLARVQLQAWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTIPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLV"
     gene            complement(8647..10875)
                     /gene="crfC"
                     /locus_tag="RH033_003981"
     CDS             complement(8647..10875)
                     /gene="crfC"
                     /locus_tag="RH033_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HDV2993095.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDHLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGIAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRVRHELANNGKLPPPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQIEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSGSLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQIKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQDAMDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSALQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2737 a         2858 c         2662 g         2631 t
ORIGIN      
        1 catacaatta tataaccatt tcccggtatc gcttcgcttt agcgagaact ggtcttttat
       61 tcgcactcag gagtacatgt atgaggattt gcagcgacca accttgtatt gttttattga
      121 ctgaaaaaga tgtctggata agggtgaatg ggaaagaacc tattagcctt aaagctaacc
      181 acatggcgtt attaaattgt gaaaataata ttatcgacgt ctcctctctt aataacactt
      241 tggttgctca tattagtcac gacatcatca aagattacct ccggtttctg aataaagatc
      301 tctcgcaaat accagtatgg caacgtagcg ctacgcccat cctcaccctt ccatgcctga
      361 cgccagacgt ctttcgcgtt gccgcgcagc atagcatgat gcccgctgaa actgagtcag
      421 aaaaggaacg aacacgtgca ttattattca ctgtgctatc ccgttttctc gacagtaaaa
      481 aattcctttc actaatgatg tatatgttac gtaattgtgt aagtgacagc gtttatcaaa
      541 ttattgaaag cgatatccac aaagactgga atcttagtat ggtagccagt tgtttatgtc
      601 ttagcccaag tctgttaaag aaaaagctga aaagcgaaaa caccagttat agccaaataa
      661 tcaccacctg ccgcatgcgt tatgccgtaa atgaattaat gatggacggt aaaaatatct
      721 ctcaggtatc acagtcctgc ggctacaaca gtacgtcgta ctttatttct gtctttaaag
      781 acttctacgg tatgacgccg ctgcattatg ttagtcagca cagagaacgc actgtcgcct
      841 gatttttaac cttaacgaag agctatatta ataacggcat cagcgataac ccggccgata
      901 ataattcaac tatcgaatgc aggcgtatga tatgacgtaa tttattgtca cgaagctcgc
      961 cttcgcagga gtttaattat gtcttcggat gctgatgctc acaaagtggg cttaatcccc
     1021 gtcaccctga tggtgtcggg gaatattatg gggtcaggtg tttttctgtt acctgcaaac
     1081 ctggcctcca ctggcgggat tgccatttat ggatggttgg tgacgattat cggtgcgctg
     1141 gggctctcga tggtatacgc caaaatgtcg ttcctcgacc caagtcctgg tggttcttac
     1201 gcttacgccc gccgctgctt tggcccgttt ctcggttatc aaaccaacgt cctctactgg
     1261 ctggcctgct ggatcggcaa tatcgccatg gtggtcattg gcgtaggata tttaagttac
     1321 ttcttcccga ttctgaaaga cccattggta ttaaccatca cctgcgtcgt ggtgctgtgg
     1381 atcttcgtcc tgctgaacat tgtcggtccg aaaatgatca cccgcgtaca ggccgttgct
     1441 accgtgctgg cgctgatccc catcgtcggg attgccgtat ttggctggtt ctggttccgt
     1501 ggtgaaacct atatggcggc atggaacgtc agctgtctgg gcaccttcgg tgcgattcaa
     1561 agtaccctta acgttacgct gtggtcgttc atcggtgtgg aaagtgcctc cgttgccgca
     1621 ggtgtggtga aaaacccgaa acgcaacgtc cctatcgcca ccattggcgg ggtattgatt
     1681 gctgccgttt gctatgtact ttctaccacc gcgattatgg ggatgatccc taatgccgca
     1741 ctgcgcgttt ctgcttcgcc attcggtgat gccgcacgga tggcgttggg tgacaccgcc
     1801 ggggccattg tttccttctg cgcagctgcg ggttgcttag gttcactggg cggctggacg
     1861 ttgctggcgg gtcaaacggc gaaagccgct gccgatgacg gactgttccc accgattttt
     1921 gcccgtgtaa ataaagcggg cacgccagtg gcggggctga ttatcgtcgg tattttgatg
     1981 accatcttcc agctcagtag catttcgcca aacgcgacca aagaattcgg tctggtttct
     2041 tccgtgtcgg tcatctttac actggtgcca tatctttaca cctgtgcggc gttactgctg
     2101 ctcggccacg gtcactttgg taaagcacgc ccggcatacc tggcagttac taccattgcc
     2161 ttcctctact gcatctgggc cgtagtaggg tccggcgcga aagaggttat gtggtcattt
     2221 gtcactctga tggtaatcac cgccatgtat gcgctgaatt acaaccggct gcataaaaac
     2281 ccgtatccct tagatgcacc aataagcaaa gattaattcc ccttaatcaa gcaaacataa
     2341 aagccaacct taagaactta aggttggctt aattttgctt tgcgagcata tgcgcacttt
     2401 gttcgatgga aacaccgtga tgttgaagcg cctactaaaa agaccctctt taaatttatt
     2461 ctcctggtta ttacttgcta ctttttatat ctcagtctgc ctgaatatcg ccttttttaa
     2521 acaggtgcta caggtgttac cgctggattc actgcataac gtgctggttt tcttgtcgat
     2581 gccggtcgtc gcattcagcg tgattaatat tgtcctgaca ctaggctctt tcttatggct
     2641 taatcgtcca ctcgcctgcc tgtttattct ggtcggtgcg gctgcacaat atttcatcat
     2701 gacctatggc atcgtcatcg accgttcaat gatcaccaat atcattgata ctacgccggc
     2761 ggaaagttat gcgttgatga cgccacgaat gttattaacg ctggggttaa gtggcgtgtt
     2821 ggccgcgatt attgcctgct ggatcaaaat caaacccacc acctcacgcc tgcgcagcgt
     2881 tcttttccgc ggggccaaca tcctgatttc tgtactgctg attctgctgg ttgccgcact
     2941 gttctacaaa gactatgcct cgctgtttcg caacaacaaa gaactggtga aatccttaag
     3001 cccgtccaac agcattgttg ctagctggtc gtggtactct caccagcggt cagccaacct
     3061 gccactgata cgcattggtg aagatgctca ccgcaaccca ttaatgcaaa atgggaaacg
     3121 caaaaacctg accattttga tcgtcggaga aacgtcgcgc gcagagaatt tctcgctgaa
     3181 tggttatcca cgcgaaacca atcctcgcct ggcaaaagat aacgtggtct atttccctaa
     3241 taccgcatct tgtggcacag caacagccgt ctcagtgccg tgcatgttct cggatatgcc
     3301 gcgtgagcac tacaaagaag agctggcaca gcaccaggaa ggcgtgctgg atatcattca
     3361 gcgagcgggc atcaacgtgc tgtggaatga caacgatggc ggctgtaaag gtgcctgcga
     3421 ccgcgtgcct caccagaacg tcaccgcgct gaatctgcca ggtcagtgca tcaacggcga
     3481 atgctatgat gaagtgctgt tccacgggct ggaagattac atcaataacc tgcagggtga
     3541 tggcgtgatt gtcttacaca ccatcggcag ccacggcccg acctattaca accgctatcc
     3601 gccgcagttc aggaaattta ccccaacctg cgacactaac gagatccaga cctgtaccaa
     3661 agagcaactg gtgaacactt acgacaacac gctggtttac gtcgactata ttgttgataa
     3721 agcgattaat ctgctgaaag aacatcagga taaatttacc accagcctgg tttatctttc
     3781 tgaccacggt gagtcgttag gtgaaaatgg catctatctg cacggtctgc cttatgccat
     3841 cgccccggat agccaaaaac aggtgccaat gctgctgtgg ctgtcggagg attatcaaaa
     3901 acggtatcag gttgaccaga actgcctgca aaaacaggcg caaacgcaac actattcaca
     3961 agataattta ttctccacgc tattgggatt aactggcgtt gagacgaagt attaccaggc
     4021 tgcggatgat attctgcaaa cttgcaggag agtgagtgaa tgaaaattct gattgttgaa
     4081 gacgatacgc tgttattgca gggattaatt ctggcggcgc aaaccgaagg ctacgcgtgc
     4141 gatggcgtga caaccgcgcg gatggcggaa caaagccttg aggccggtca ttacagcctg
     4201 gtggtactgg atttagggtt acccgacgaa gatggactgc attttctcgc ccgtatccgg
     4261 cagaaaaaat acaccctgcc ggtactgatc ctcaccgctc gcgatacgct gaccgacaaa
     4321 atcgccgggc tggatgtcgg tgccgatgac tatctggtga agccttttgc gctggaagag
     4381 ttacacgccc gtatccgcgc cctgctacga cgccataata atcagggcga aagtgagctg
     4441 attgttggca atctgacgct gaatatgggt cgccgtcagg tatggatggg cggtgaagag
     4501 ttgattctga cgcccaaaga atatgctctg ctgtcacggt taatgctcaa agcaggcagt
     4561 ccggtgcatc gggaaattct ctacaacgac atctataact gggacaatga accctcgacc
     4621 aacaccctgg aagtgcatat ccacaatctg cgcgacaaag tgggcaaagc ccgtatccgc
     4681 accgtgcgcg gatttggcta tatgctggtc gcaaatgagg aaaactaatt gaatctgatg
     4741 cgttttctgc gccgaccaat atcgctgcgc caacggctga tattgaccat cggggccatt
     4801 ttgttggtgt ttgagctgat cagcgtcttc tggctatggc atgaaagtac cgagcagatt
     4861 cagctgtttg agcaggcgct acgcgacaat cgcaacaacg atcgccacat catgcgtgag
     4921 atccgcgagg cggtcgccag cctgattgtc cccggcgtct ttatggtcag cctgacgcta
     4981 tttatctgct atcaggcggt acgccgcatc acccgcccgc tggcggagct gcaaaaagag
     5041 ctggaagcgc gcaccgccga caacttaacg cccattgcca ttcacagcgc caccctcgaa
     5101 atcgaagcgg tggtttcagc gttaaacgat ctggtcagtc gcctgaccag cacgctggat
     5161 aacgaaaggt tgtttaccgc tgacgtcgcg cacgaactgc gaacgccact ggcgggggtg
     5221 cgtttgcatc tggaactgct ggcgaaaacg catcacattg atgtagcacc gttagtggct
     5281 cgacttgatc agatgatgga gagtgtctcc cagctgctgc aactggcgcg tgccggacag
     5341 tcattttctt ccgggaatta tcaacatgta aaactgctgg aagatgtcat tctcccctcg
     5401 tatgacgaac tcagtaccat gctcgaccag cgacagcaaa ccctgctatt gccagagagc
     5461 gccgccgaca tcaccgttca gggtgatgca accctgctgc ggatgttatt gcgaaacctg
     5521 gtagaaaacg cccatcgtta cagcccgcaa ggcagcaaca ttatgattaa gctgcaagaa
     5581 gatggcggag cagtcatggc cgttgaagat gaaggaccgg gtattgatga gagtaaatgc
     5641 ggggagttga gtaaagcgtt tgtacgtatg gacagccgtt atggcgggat tggtctgggg
     5701 ttaagtattg tcagccgcat tacacagttg catcacgggc agtttttcct gcaaaaccgg
     5761 caagagactt ccggcacgcg ggcctgggta cggctgaaga aagatcagta cgtggtaaac
     5821 cagatataaa gaaagctgct gaccaccagc acgctgaaca cggtagttaa actttcataa
     5881 acacggtttt tcattgcact ctcctccgtt aacctggagg agagtatgcg cgtcagagat
     5941 taagtgaacc ttaagagttc aattggcctg ataagacagc gtcacatcag gccatccgtt
     6001 tcagcttatt catcaattcg cggatgttgc tgcaccaggc gggtacgttt cgcctgcaaa
     6061 tcggcaatct cgtggtcaat atcatcgatt ttctgctcga tattatcgta atgctcgacg
     6121 agaatttcct tcgcttcctg tatatctgac gccgccggtg tcgcaccttt caacggacga
     6181 tttgccgtct ctttcatggt tacgccggtg attaaaccaa ccaccgccac taccatcagg
     6241 taataggcag gcatcatcag attctgcgag ctttcgacca gccaggccgc cagtgttggc
     6301 gtcagaccgg caaccagcac cgaaatatta aatgccgccg ccagcgcgct gtaacggata
     6361 tgcgtcggga acatcgctgg caaggtagaa gccataacgc ccgtaaagca gttaaggatc
     6421 accgccagca tcagtaaccc ggcaaaaatc aggccgatga cgttactgtt aatcagaata
     6481 aacgccggga tcgccaacac aaacagtgca acactaccaa gtagcacaaa cggacgacgg
     6541 ccaaaacggt cactcagcaa gcccatcacc ggctggacaa acagcatacc gatcataatg
     6601 gcgataataa tcagcacccc gtggtcttcg gagtaatgca ggttatgcga caaataactc
     6661 ggcatatagg tcagcaacat gtagtaagtc acgttggtgg caattaccag accaatacat
     6721 gtcaacaggc tgcgccagta tttagtggca atctctttaa acgagacttt cgggccatcc
     6781 tgcaaacctt cgcggtcgcc ctgttccagt ttatcaacat gctgctggaa cgccggagtc
     6841 tcttccagcg catggcgcag gtaaagcccg ataatcccta acggcagagc aataaagaac
     6901 ggaatacgcc agccccagtc gaggaagttc gcttcgccga caatggtcga aattaacacc
     6961 accacgcccg cacccagcac aaacccggca atagaaccga agtccagcca gctgcccata
     7021 aagccacgtt tacggtcagg ggagtattcc gcaacaaata tcgacgcccc ggtatattca
     7081 ccgccgaccg agaaaccttg tgccatctta cagatcaaca gcagaatcgg tgcccaaata
     7141 ccaatcgtgt cgtaggacgg tattaagcca atacagaacg tactgatcga cataatcaca
     7201 atagtgatag cgaggatctt ctggcgacca tatttatcgc ccaacatacc aaagaagagt
     7261 ccaccaagcg gtcgaatcag aaagggaacg gagaaagtgg caagtgcagc aaccatctgc
     7321 acgctggggt cagcccccgg gaaaaaaact ttacctaatg cgtaagcaac aaaaccataa
     7381 acaccaaaat caaaccattc cattgcatta cccagtgatg ctgcggtaat ggctttacgc
     7441 agtttaccgt catcaataat ggtgacatca cgaagggtaa ttggttttac ttttttcctt
     7501 ttcagcatag ctttcctcgc agagttggcc catttccgca aacgccagaa aaatcagcgc
     7561 gacggccgca aaaggggagc gggaattgca ggatgcaatc tctgtaacaa gcgtagcggg
     7621 tttgcttaca ccctccggtt aaggaatgta caatcaaacc agttgacgcc tgaattgggc
     7681 agttaatgac ctttttaccc taccagtgtt taaatttgtg atcaacttca cacatgtatt
     7741 tcgtttagga ctcattggtg taactgatta tttacaccaa atttacagag aagatttcct
     7801 cgaccattgc ccccgccaga cgctgcgctg aacagagtcg tggcatcccc agcgcgacag
     7861 tttgccaacg ttgggtcact gaccagcaaa cgggttgttt atctgccgtg gtccactttc
     7921 ccagccaact taaaaaatca gcatgatccc cgcccgtcgg aatggtcgaa gaggcaaacc
     7981 actgatggta atagtgacgc gtggtcgggg ccgcattgat actctctgcc aaatgttgtg
     8041 tcgctttctc gcgcaaatca cccagcaaca acgccaccgc ttgcgggctt cccagacggc
     8101 aggcatcgcc aaatgccccc cagcgcaact cctccagaac gataaaacag cgcccaggca
     8161 acgaccaggc gttatacgcc ccagcacgcc agcgggtaaa aatttgctcg ctctgttcac
     8221 ccgcctgcac cagtaggcct cgttgcgtta acgccttttc cttccagcga caacgggctg
     8281 aaaaccactc atacaaggcg gcaatttgct gggggagctc agggaaagca gagtgctggc
     8341 ggtgatccat tttttgcagc agcgccagat gttgcgcgat ctgcgtcagc gccagatgac
     8401 cgggatccag catcgacgcc agtttttcca ccaatgtcag acgcaggcta agcggcgcat
     8461 ttaacatccc gccgttcgtc catgcctgaa gctgcacacg cgcaagaagt tcctgcatca
     8521 gtcgttcacg aaactgctgc tgatgggctt gtggatggcg ggcatcatcg ccctgcgcca
     8581 gatccactaa aaagcgggga tgaatcgact ccagcacgcg accggggccg tccagtaacg
     8641 tcttggtcaa tatcgttctg ccgtgaaaag tgtttgaata tcatcgcgta acagctgagc
     8701 gtcggtgtaa atccagccat gagtcatcac agtctgctgc aattgctgct gcatcagcct
     8761 gaccaccgat tcattttgtt gacgcagagc caggctttcg cgtaaacgcg tctgtaattc
     8821 cgtcaaacat gaagcgaact cagcgaaaaa agtattcatg cctgccgtaa cagattcatc
     8881 gacctgctct gccagaactt tagccatttg ttggcaataa agatcgactt ctgcgcttaa
     8941 tgctcgttgc aacgcactgt aatcaaccgt ctctgtcggg gatttctcat ttccccgtcc
     9001 ccagtcgggc tgattcaacc agcgcgaaaa agtctcacgc acaacgccta aacgcgtgct
     9061 ctgctcgtcc attgcgtcct ggcgcgaaat gacttcactg aacagctggc gagtgttgaa
     9121 gtggggaact acgccgtgaa aaacaggaaa atgaaaccca ggacgaaacc ctgactcgct
     9181 caattccatt ttgatttgtt gctcaatggg gcgaataaca tcggttaaca cccggcaaag
     9241 ggtggattcc agctcggcaa aacgcagcgt aaagtcgcga ctaatggtgt tctgcgccgt
     9301 ctgtaacagt gtctcacagc aggtacgaat ctcgcttaat gaccccgaat catcctgaaa
     9361 caaggcggct aactgcgcat tcagcgcatc ttgctgttga cgcagaaagt ggttggcgga
     9421 ggtcagggcc agctcgattt catgtttaat ctcgccgctc acctgcgcct gattgagttg
     9481 caatagctgc aaactttctt cgatctgatg gatattttgc cgcaattgtt cacaagcgac
     9541 gtttaacccg tgcgcacgaa aatccaggta ttcccgcgcc tgctgcgcgt aattcaacag
     9601 tttatgcgcg gcagatcgca gagcatacaa cgaggcgtta gcgtaagcgg catgaagcaa
     9661 cgcctgaatt ggctgggcga acagcgaatc ttcccacaac tgatcggcag catgacgaat
     9721 atgttcgagg tccgccagat cggcatgacg ccagcgcctg ccgagcgcgg catgggcaaa
     9781 atcttccacc cagcgttgtt gctctggcgg tggtaactta ccgttgttgg ctaactcatg
     9841 gcgcacccga ttcgccaggt agccccacat cgacgacacc ggaaatatct gctgtggcgt
     9901 aatacaacct ttcatcagcg tcccggaaat cagtgcccgc acctggtcgg cgtcgtcact
     9961 gttacgatcc tgttgatcga acttattgac cagcacatac aacggcaccg attgccccac
    10021 cgccaaaatt gcctcacgga cctcttcatc ggagatcgat ttcagttgcg tataatccag
    10081 caccgccagt accgccgagg cgcgtgccag ctgctggtta agcatttttt gcagatgcgg
    10141 ttgcccggct tcatttggcc cgggggtatc cagtaacgtc aactgaccgg gataactctc
    10201 cagccccgcc agatggacaa actccacttc aatcacggga atatgctcaa tggcggcgta
    10261 agcagaaaaa ggaaaatcga cgtccagcgc cttcgccagt cgcaccaaat cattcaaact
    10321 tttcagacaa tgaaaaatag gttgagcacc cagataatat ttttcgaaag cgatgccatt
    10381 ttcaatccgc tgcataagcg cacgcatatc tttatctatt tccagcacat cggtcagatg
    10441 cttaatatcg caatcacgca ggcgctgttg taattgttga attaaatggt cgattggcgc
    10501 gacatgtgaa aaatgcagta ccggttcttt ttgcccgggc gtatggcgaa taagcgttgg
    10561 cagcgcagtc attgggcgat tgcgattagg cagaacctcc gtaccaacaa tggcattaat
    10621 ggtggttgat ttccctgctt tcatagtacc gacaatcgcc agcaccattt ccagtcggga
    10681 aattttacgc aactcattat tcagcatcgc gtgacgttcg gcgatattag gctgactcca
    10741 gggtaaagcc agttgtggcg catcgtctcc gggtacagag agaggcattt tttccagtaa
    10801 ctgcaactgt tggcgagaaa gctgtaacag gcgttcagcc tcctgactta actcatacag
    10861 ggtctgtgtg tacatagaaa atttttcc
//
LOCUS       DAPHCV010000135        10806 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.136, whole genome shotgun sequence.
ACCESSION   DAPHCV010000135 DAPHCV010000000
VERSION     DAPHCV010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.136"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..212)
                     /gene="tnpB"
                     /locus_tag="RH033_003982"
     CDS             complement(<1..212)
                     /gene="tnpB"
                     /locus_tag="RH033_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDV2993096.1"
                     /translation="MISIPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRL"
     gene            complement(212..862)
                     /locus_tag="RH033_003983"
     CDS             complement(212..862)
                     /locus_tag="RH033_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001690050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDV2993097.1"
                     /translation="MNARKAVLADNPELILRVLQLRFDESLSYPRISAQTGISKTAIF
                     SLVRRFHQVFTDWPLSGEYSCVQLARALFPGRYPSAPTVTQTVKTEKTRRNQFSPEFK
                     WRLVQQTLLPGACVAQIARENGINDNLLFNWRRLWRNGGLQSPGEHETSLLPVTLTPE
                     PDNKIPAPVQIPEQTNTLSDSLCCELVLPAGTLRLKGKLTPALLQTLIREIKGSSH"
     gene            complement(1154..2518)
                     /gene="pgtP"
                     /locus_tag="RH033_003984"
     CDS             complement(1154..2518)
                     /gene="pgtP"
                     /locus_tag="RH033_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate transporter PgtP"
                     /protein_id="HDV2993098.1"
                     /translation="MLSIFKTGQAADSVPAEKIQVTYRRYRMQALLSVFLGYLAYYIV
                     RNNFTLSTPYLKEQLDLSATQIGVLSSCMLIAYGISKGVMSSLADKASPKVFMACGLV
                     LCAIVNVGLGFSTAFWIFAVLVILNGLFQGMGVGPSFITIANWFPRRERGRVGAFWNI
                     SHNVGGGIVAPIVGAAFALLGSEHWQSASYIVPACVAIVFAVIVLILGKGSPRQEGLP
                     SLEEMMPEEKVVLNTRQTVKAPENMSAFQIFCTYVLRNKNAWYVSLVDVFVYMVRFGM
                     ISWLPIYLLTVKHFSKEQMSVAFLFFEWAAIPSTLLAGWLSDKLFKGRRMPLAMICMA
                     LIFICLIGYWKSESLFMVTIFAAIVGCLIYVPQFLASVQTMEIVPSFAVGSAVGLRGF
                     MSYIFGASLGTSLFGIMVDHIGWHGGFYLLGCGIICCIIFCWLSHRGAIELERHRAAY
                     IKEH"
     gene            2917..4134
                     /gene="pgtC"
                     /locus_tag="RH033_003985"
     CDS             2917..4134
                     /gene="pgtC"
                     /locus_tag="RH033_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate transport regulator PgtC"
                     /protein_id="HDV2993099.1"
                     /translation="MILLLLLMDTGAVRAQRNELVMATTFSPGATAWIIQRWQTEPES
                     VMIRTLNRTSASLEQLLYTANVENVDLILTSSPMLLQHLQEHQKLAPFDDAPAESQNL
                     VPESIRATSVAVAISGFGLLINRPALSVKHLPAPADWDDLALPIYQDALLMSSPSRSD
                     TNHLMVESLLQQKGWVKGWETLLTSAGNLVTISSRSFGVADKIKSGLGVAGPVIDNYA
                     NLLLNDPHLSFTYFPRSAVSPTYVAILKKSPHADAARRFIHYLLSPKGQRILADANTG
                     KYPVTPLAADNPRATQQQLLMNQPPLNYHLILKRQRLVQRLFDTAISFRLAQLKDAWR
                     ALHSTEARLKKPLPEIRALLTQVPVAAASSEDPVWLAQFDNKSFTEQQMMKWQLWFLN
                     NQRLAIKKLEELK"
     gene            4131..6140
                     /gene="pgtB"
                     /locus_tag="RH033_003986"
     CDS             4131..6140
                     /gene="pgtB"
                     /locus_tag="RH033_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PgtB"
                     /protein_id="HDV2993100.1"
                     /translation="MKNITLWQRLRQVSISTSLRCAFLMGALLTLIVSSVSLYSWHEQ
                     SSQIRYSLDEYFPRIHSAFLIEGNLNLVVDQLNEFLQAPNTTVRLQLRTQIIQHLDTI
                     ERLSRGLSSRERQQLTVILRDSRSLLSELDRALYNMFLLREKVSELSARIDWLHDDFT
                     TELNSLVQDFTWQQGTLLDQIASRQGDTAQYLKRSREVQNEQQQVYTLARIENQIVDD
                     LRDRLNELKSGRDDDTQVETHLRYFENLKKTADENIRMLDDWPGTITLRQTIDELLDM
                     GIVKNKMPDTMREYVAAQKALEDASRTREATLGRFRTLLEAQLGSTHQQMQMFNQRME
                     QIVRVSGGLILVATALALLLAWVFNHYFIRSRLVKRFTLLNQAVVQIGLGGTETTIPV
                     YGNDELGRIAGLLRHTLGQLNVQKQQLEQEITDRKVIEADLRATQDELIQTAKLAVVG
                     QTMTTLAHEINQPLNALSMYLFTARRAIEQTQKEQASMMLGKAEGVISRIDAIIRSLR
                     QFTRRAELETSLHAVDLAQMFSAAWELLAMRHRSLQATLVLPQGTATVSGDEVRTQQV
                     LVNVLANALDVCGQGAVITVNWQMQGKTLNVFIGDNGPGWPEALLPSLLKPFTTSKEV
                     GLGIGLSICVSLMEQMKGELRLASTMTRNACVVLQFRLTDVEDAK"
     gene            6130..7377
                     /gene="pgtA"
                     /locus_tag="RH033_003987"
     CDS             6130..7377
                     /gene="pgtA"
                     /locus_tag="RH033_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001765251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PgtA"
                     /protein_id="HDV2993101.1"
                     /translation="MLSSEYSILLIDDDADVLDAYTQLLEQSGYRVFACNNPFEAQAW
                     IQPDWPGIVLSDVCMPGCSGIDLMMLFHQDDQQLPILLITGHGDVPMAVDAVKKGAWD
                     FLQKPVDPGKLLSLVEEALRQRQSIIARRQYCQQTLQVELIGRSEWINQYRRRLQQLS
                     ETDIAVWLYGAPGTGRMTGARYLHQFGRNAQGEFVYRELTPDNAPQLNDFIALAQGGT
                     LVLSHPEHLTREQQYHLVQLQSQEHRPFRLIGIGDTSLVELAASNHIIAELYYCFAMT
                     QIACLPLTQRPDDIEPLFRHYLCKACQRLNHPVPEVGKEMFKEMMRRMWPNNVRELAN
                     AAELFTVGILPLAETANPLMHVGTPAPLDRRVEDAERQIITEALNIHQGRINEVAEYL
                     QIPRKKLYLRMKKYGLSKEHYKN"
     gene            7506..7997
                     /locus_tag="RH033_003988"
     CDS             7506..7997
                     /locus_tag="RH033_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993102.1"
                     /translation="MTSRVGNIQSFVHKVVEHWPNLLAFHFTLYSAEGIYGQQIQTFC
                     SSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHPRVGVMA
                     DEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSFMSQVIAT
                     IIR"
     gene            8086..8724
                     /locus_tag="RH033_003989"
     CDS             8086..8724
                     /locus_tag="RH033_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDV2993103.1"
                     /translation="MRTRIDYLADKYSFTELNERPHLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLIRTPQQIAALREEWDISQKNVLFNDKRFGCVYSLKS
                     SLSGVPDTYRYHLSHRIRRVVANENTSLPYQQIAREVKAPRERLKYALETGLQVTALD
                     GLFWFGSQRIAADIQRLRKSGMRITTSAISVYDSITATRRLCPVYRIVANNK"
     gene            9061..10680
                     /locus_tag="RH033_003990"
     CDS             9061..10680
                     /locus_tag="RH033_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003845607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDV2993104.1"
                     /translation="MARIPTKKAKAKKGFEETLWDAANQLRGSVESSEYKHVVLSLVF
                     LKFISDKFETRRKKMIADGQADFLEMEVFYQQDNIFYLPEEARWSFIKQNAKQDDIAV
                     RIDTALSTIEKRNQTLKGALPDNYFSRQNLETKKLASLIDTIDNIETLAHETDVETLS
                     KEDLVGRVYEYFLGKFAATEGKGGGEFYTPKCVVTLLTEMLEPFQGKIYDPCCGSAGM
                     FVQSVKFVESHQGKSRDIALYGQELTATTYKLAKMNLAIRGLSANLGERPANTFFSDQ
                     HPDLKADYILANPPFNLKDWRNEAELTKDPRFAGYRMPPTGNANYGWILHMLSKLSAN
                     GTAGFVLANGSMSSNTSGEGEIRAQMIENDLIDCMIALPGQLFYTTQIPVCLWFMTKS
                     KAADPAKGYRDRQGETLFIDARNLGTMISRTTKELTTEDIATIANTYHAWRSTQEELA
                     ARIARGDSKLEKYEDQAGFCKVATLQDIKDNDYVLTPGRYVGAAEQEEDGVAFETKMR
                     ELSKTLFEQMKQAEELDRAIRQNLEALGYGE"
BASE COUNT         2994 a         2545 c         2659 g         2608 t
ORIGIN      
        1 aggcgtttgg tgaagaggca cagtccgtcg ctgtcagccc acaacacttt tatctggtca
       61 ccccggcgtc cgcggaagat gaacaggtgt ccggagaacg ggtcatcctt caggacgttc
      121 tgaacttttg acgccaggcc gttaaagcca tttcgcatat cggtgatacc tgcaaccagc
      181 cagatacgcg aaccggcagg gatagatatc atcagtggct gctccctttt atttcacgga
      241 taagtgtctg taataacgcc ggcgtcagtt tacctttcag cctgagagtt ccggccggca
      301 gaaccagctc acagcacaga ctgtcggaca gtgtatttgt ctgttcaggt atctgcactg
      361 gtgccgggat tttattatcc ggctccggag ttaacgtcac gggaagtagc gatgtttcat
      421 gttcgccggg cgactgcagg ccgccgttac gccagagacg ccgccagtta aagagcaggt
      481 tatcgttgat tccgttctca cgagctattt gtgcgacaca ggcaccgggt aaaagagttt
      541 gctgaacaag tctccattta aactccggtg aaaattggtt ccggcgggtt ttctctgttt
      601 tcacagtctg agttactgtc ggggctgaag ggtatcgccc cgggaacaga gcccgggcca
      661 gttgcacgca ggaatattcc ccggaaagag gccagtcagt gaatacctgg tgaaatcgcc
      721 tcaccagaga aaaaatggcg gttttgctga taccggtctg cgcagaaatg cgcgggtacg
      781 acagtgactc gtcaaatctc agttgtagca cacgcaggat caattccgga ttgtctgcca
      841 gtaccgcctt tcgtgcattc atcttaaatg tccctttact gcgaaaatgg acattagtat
      901 cggaaacagg aaagggaggc gaaagacggt ttaaatgaga cggttactgt tttttagcat
      961 tttcaatgca taacaattgc gaattatatg cgttatctga ttgagacaaa agatatccat
     1021 ttttctcatg acaataccat ctccatttat tgtcatcagt tttataaaat ttccatatta
     1081 atctctgatt gccagatatc ttataaatca taatatgctc cactccccag ggaaacgacc
     1141 ctggggaagg taatcagtgc tcttttatat atgcggctct gtgacgttca agttcaattg
     1201 caccacgatg tgataaccag caaaaaatga tgcaacaaat aataccgcag ccaagaagat
     1261 aaaatccgcc atgccagcca atatgatcga ccataatacc aaacaggctg gtgcccagag
     1321 atgcaccgaa gatatagctc ataaaaccac gtaaacctac cgcggagcca acagcaaagc
     1381 tgggcacgat ctccatagtc tgaacagaag ccagaaattg tggaacgtaa atcagacaac
     1441 caacaatggc agcaaaaatt gtcaccataa acagtgattc acttttccag tagccaatca
     1501 ggcaaatgaa aatcagcgcc atacaaatca tcgccaatgg catacgacgc cctttaaaca
     1561 gtttgtctga caaccaaccg gcaagtagcg tggaagggat tgcggcccat tcaaaaaata
     1621 aaaacgcgac gctcatttgt tctttagaaa aatgtttcac cgtcagcaga taaataggca
     1681 accagctaat catcccgaag cgtaccatgt atacaaatac gtcaaccagt gagacatacc
     1741 aggcattttt attgcgtaat acataagtgc agaaaatctg aaaggcactc atgttttctg
     1801 gtgcttttac cgtctgtcgg gtattcagga cgactttttc ttccggcatc atctcttcca
     1861 gagagggtag accttcctga cgtggggaac ctttaccgag aatcagcaca attaccgcaa
     1921 aaacgatggc cacgcaggcc ggaacgatat agctcgcact ttgccagtgc tcgctgccga
     1981 gtagggcaaa agcggcacca acaatgggtg caacaatacc accaccgacg ttatgagaga
     2041 tattccagaa agcaccaacc cgaccacgct cccggcgagg gaaccagtta gcaatagtga
     2101 tgaaagaagg accaacgccc attccctgga aaagaccatt cagaataacc aataccgcaa
     2161 aaatccagaa tgcggtgctg aatcccaggc caacgttaac gatggcacat aacaccagcc
     2221 cacacgccat aaagactttc ggactggctt tatcggcaag gctactcatc actcctttgc
     2281 tgataccata ggcgataagc atacagctac tcagtacgcc aatttgtgtg gcgctgagat
     2341 ctaattgctc tttaagataa ggcgtcgata aggtgaaatt attacgcacg atatagtatg
     2401 caagataccc cagaaataca ctaagtaacg cctgcatacg atagcgacga tatgtcacct
     2461 gaattttctc cgccggaaca ctatccgccg cttgccccgt tttaaatatt gataacatgt
     2521 cttcacctgt attttatatt gatatgaaca cctggagtat tctgacaatg agagttatat
     2581 aattgagatg aaacatcaaa ctgaaaaaat gtgtcacaat tgacacaaat taataaaatc
     2641 aaagcatgtt tttgacccat tttattgatg catagacagg gtagatttat gctttatctg
     2701 ttttattgtt agcatgtgcg gatattaaca aatctcatgc tgcttatcgg gtaatcactg
     2761 attaatatat taacgctctc tttgagcaga tgtgttatca gacgaagtgt ctgaaacggg
     2821 taatttttgt ctcaatgcga tttttgcggt gtattaatat ttatgacctg gatcacaaac
     2881 cgatttttta gctggtgcga aatcggcatc aaaaccatga ttcttttact gttgttgatg
     2941 gataccgggg ccgtcagggc tcaacgaaat gaactggtga tggccaccac attctcaccc
     3001 ggagctaccg cgtggataat acagcgctgg caaacagagc ctgagtcggt aatgatccgt
     3061 acgcttaacc gcaccagtgc ctcactggaa cagttgcttt atacggccaa cgtagaaaac
     3121 gtcgatctga tcctgacctc atcaccaatg ctgctccagc accttcagga gcaccagaaa
     3181 ctggccccgt ttgatgatgc acccgcagaa agccaaaacc tggtgccgga gtcgatccgt
     3241 gcaacctccg ttgcagtagc aatatcaggt tttggtctgc ttattaatcg tcctgcgctt
     3301 tctgtaaaac accttcctgc ccctgctgac tgggacgatc ttgctttgcc gatctatcag
     3361 gacgctttat tgatgagtag tccgtcgcgt tcagatacta accatttaat ggttgagtca
     3421 ttactacagc aaaaaggctg ggtgaaagga tgggaaacgt tgctgacaag tgcaggaaac
     3481 ctggtgacaa tctcctcccg cagttttggc gtagccgata aaataaaaag cggactcggc
     3541 gttgccggcc ccgttatcga taactatgct aatttactgt taaatgaccc ccatctctct
     3601 tttacctatt tcccccggtc tgcggtatct cccacctacg ttgcaattct caagaaaagc
     3661 ccacacgccg atgcagcccg tcgctttatt cactacctgt taagtccgaa aggacagcgt
     3721 attctggccg atgccaatac aggaaaatat ccggtgacac cacttgccgc agacaatccg
     3781 cgggcaacgc aacaacagct cctgatgaat caacccccgc tgaattatca ccttatcctg
     3841 aaacgtcagc gtctggtgca gcgcttgttt gatacggcaa ttagctttcg gttagcacag
     3901 ctaaaagatg cctggcgagc gctgcacagt accgaggcac gcctgaagaa accattacca
     3961 gaaatacgtg cactgttaac acaagtgccc gttgcagcgg ccagcagtga agatcccgtc
     4021 tggctggcac agttcgataa caaaagtttt accgagcagc aaatgatgaa atggcaactc
     4081 tggtttctga ataaccagcg cctggcgata aaaaaactgg aagagctgaa atgaaaaaca
     4141 taacgctgtg gcagcgttta agacaggtca gtatcagtac cagcttacgt tgcgcatttc
     4201 tgatgggggc actactgacc ctgattgtca gtagtgtcag tctgtattca tggcacgaac
     4261 aaagctcaca aattcgttac tcgctggatg agtattttcc ccgtattcac tctgctttcc
     4321 ttattgaagg gaacctgaat ctggtggtag accagctaaa tgaatttttg caggctccca
     4381 acaccacggt gcgattgcaa cttcgtaccc agattattca gcatctcgac accatagaac
     4441 ggcttagtcg gggactgtca tcccgggaac gccaacaact gacggtcatt ttgcgggaca
     4501 gtcgatcact gttatccgag ttggatcgtg cgctttacaa catgttttta ctacgggaaa
     4561 aggtgagtga gctatcagcg cggattgact ggttacacga tgactttact accgagctta
     4621 attctttagt gcaggatttc acctggcagc agggaacgct gctggatcaa atcgcctccc
     4681 gacagggcga tacggcgcaa tatctgaagc gatctcgtga agtgcaaaat gaacagcagc
     4741 aggtttatac cctggcacgc attgaaaatc agattgttga cgatctgcgt gacagactca
     4801 atgagctcaa atcaggacgt gatgacgaca cacaggtgga aactcatctc cgttattttg
     4861 aaaatctgaa aaaaacggca gatgaaaata tacgtatgct ggatgactgg cctggcacca
     4921 ttaccctgag gcagactatc gatgaattgc tggatatggg aatcgtaaaa aataaaatgc
     4981 cggatacgat gcgtgaatat gtcgccgccc aaaaagcctt agaggatgcc agtcgcacca
     5041 gggaagcgac actgggtcgc ttcagaacgt tactggaagc gcagcttggc agtactcatc
     5101 aacaaatgca gatgtttaat caacgaatgg aacaaattgt tcgcgttagc ggtgggctga
     5161 tcctggtggc gacagcactg gcgttactgc tggcatgggt attcaaccat tattttatcc
     5221 gctcacggtt ggtgaaacgc tttaccctac tgaatcaggc cgttgtgcaa attggtctgg
     5281 gaggcacgga aacgactatt cctgtttatg ggaatgatga actggggaga attgcaggat
     5341 tattacgcca tactctcggg cagctcaatg tgcaaaaaca gcaacttgaa caagaaatta
     5401 ccgatcgaaa ggtgatagaa gccgatctgc gtgccaccca ggatgaactg attcagacag
     5461 caaagttggc ggtagtcggg caaacgatga ccacgctggc ccacgagatc aatcagccgc
     5521 taaatgcgct gtcaatgtat ctgtttacag cccgcagggc cattgaacag acccagaaag
     5581 aacaggccag catgatgctt ggtaaagccg aaggggtgat tagtcgtatt gacgccatta
     5641 tccgttcact acggcagttt acccggcgcg ccgaactgga aacatcactc catgccgttg
     5701 atttagcaca gatgttcagt gcggcctggg aacttctggc catgcgtcat cgctctctgc
     5761 aagctacgct tgttctgccg caaggtacag ccacagtttc aggtgatgag gtcagaaccc
     5821 agcaggtact ggttaacgta ctggcgaatg cgcttgatgt ttgtgggcaa ggcgctgtca
     5881 ttaccgttaa ctggcaaatg cagggtaaaa cgctgaacgt attcattggc gataatggcc
     5941 cgggctggcc tgaggcattg ttgccttcgt tattgaagcc gtttaccacc agtaaagaag
     6001 taggactggg tattggtctt tcaatttgtg tgtcgttgat ggagcaaatg aaaggggaat
     6061 tgcggctggc atcaacgatg accaggaatg cctgtgtggt actgcaattc agactaacgg
     6121 atgtggaaga tgctaagtag tgaatattcg attctgctga ttgatgatga tgctgacgtg
     6181 ctggacgcct acacacagtt acttgaacag tcgggctatc gtgtttttgc ctgtaacaat
     6241 ccttttgagg cgcaggcgtg gatacaacca gactggccag gcattgtgct aagtgatgtg
     6301 tgtatgcctg gctgctctgg tatcgatttg atgatgttat tccaccagga tgatcaacag
     6361 ctccccattc tgcttatcac cgggcacggc gatgtgccaa tggcagttga tgcggtgaaa
     6421 aaaggcgcgt gggatttttt gcaaaaaccg gtcgatcccg gcaaattgct ttctctggtt
     6481 gaagaggcac tacgccagcg tcaatcaatc attgcgcgtc gtcagtattg ccaacagaca
     6541 ttgcaggtgg agttgatagg gagaagtgaa tggataaatc agtatcggcg acgtttacag
     6601 cagttgtctg agacagatat tgccgtctgg ctctacggtg cccctggcac aggacggatg
     6661 accggtgcac gttatctgca ccaatttgga cgtaatgcac aaggcgaatt tgtctaccgc
     6721 gagctgacac cagacaatgc accacagctt aatgatttca ttgcgctggc tcagggggga
     6781 acattagtgc tcagccatcc tgaacacctg acgcgtgaac agcagtacca tctggttcag
     6841 ttacaaagcc aggaacacag accttttcgc ctgattggta ttggagacac ctcgctggtc
     6901 gagctggccg ccagcaacca tatcatcgcg gagctctatt actgctttgc aatgacacaa
     6961 atcgcttgcc tgccattaac tcaacgcccc gacgatattg aaccactgtt tcgccactac
     7021 ctttgtaagg catgtcaacg gcttaaccat cctgttccgg aagtgggaaa agagatgttc
     7081 aaagagatga tgcgccgcat gtggccaaat aatgtgcgag agctggccaa tgcagcagaa
     7141 ctgtttactg taggaatatt accgctggca gaaacagcaa acccgttgat gcacgtcgga
     7201 accccggcac cgcttgacag gcgagtggag gacgctgagc gacagataat cacagaggcg
     7261 cttaatatcc atcaggggcg tatcaatgag gttgctgaat atttacaaat cccgcggaaa
     7321 aaactctatc tcagaatgaa aaaatacggt cttagcaagg agcattataa aaattaaata
     7381 tcagtctatc cagcacagaa attatggagc cccccagtct tggtaccagt cccgggtact
     7441 gctgtaagtt tctgcaccga ttcagtaaat ttcagcaggg ataaaatagt gcagataatc
     7501 gaagcattac ttccagagtg gggaacatcc agagttttgt tcacaaagtg gtggagcact
     7561 ggccaaatct gttggcattt cacttcacgc tctattcggc agaaggaatc tatggtcaac
     7621 agattcagac attttgtagt tcctttcacc gacgggtaca cgaacgtatt acggagcata
     7681 atcacacggt cagcccatca gcgccggtgg tattacgctg gttgcgggaa caacatgaag
     7741 gagcacagat acgatgtctg ttgctgctca gccagacgag tatttgccat ccgcgagtcg
     7801 gcgtcatggc tgatgaagaa tgtgcgcaac tggtggattt actgcaacag acctggtcgg
     7861 tgataagtgc tggcggacaa tgccgggtgg aaagatgttt tcgggttgcc cggcctggtt
     7921 catcagggca atacgtggcg ttaaaaacgg ctgttcagtc ctttatgtcg caggttattg
     7981 caacaatcat ccggtgattg tcgggaggaa tatcaatgct tccgttaatt tttactggct
     8041 gataaagttt tgtctgctga atctttatac ggaattcctt tcagtatgcg tacacgaatt
     8101 gactatcttg cggataaata cagttttact gaactgaatg aacgtccaca ccttcgccgg
     8161 cagtggcagg atgttctgga ggagtgtcgg cagacagagg ccgggccaga agaacggctg
     8221 cggattgccc tgttgaatgt ggattacgtc accagttttg aactgccatt tcggttgctg
     8281 cttatccgta caccacaaca gattgcagcg ttgcgtgaag aatgggacat cagccagaaa
     8341 aatgtgctgt ttaacgataa acggtttggt tgcgtgtaca gcctgaagtc cagtctttct
     8401 ggtgtaccgg atacataccg gtatcatctg tctcaccgca ttcgtcgtgt ggttgcgaat
     8461 gaaaacacat cattgccata tcagcagatt gcccgggaag tgaaagcccc ccgggaacga
     8521 ctgaagtatg cgctggagac gggattgcag gtgaccgcac tggatggcct gttctggttc
     8581 ggtagccagc gcattgctgc agatatacag agactgagaa aatctggtat gaggattacc
     8641 acttctgcca taagcgtgta tgactctatt accgcaaccc ggagattgtg cccggtatac
     8701 cggattgtcg cgaataataa ataaacggta atgctgagtt tctccattac cattgcaaat
     8761 gactcaccag agcagactga acagcgcaga agtgggattg tggatacgtg aagtgagagt
     8821 aaggggaaaa tccacaataa tcatccatcg aacagggagg cgaactttac acgatggttt
     8881 tccgggagtg cttacccggg gttcctcacc tctggctaat ctctggattg agtcgcgata
     8941 ctccaacaaa agcaacaagc taacgcagca agaagttaac gctcatcgag agtaaaatgc
     9001 acacttttat ggcttactcg ttacaataac agccagtctg ttcagaaaac cggattcagt
     9061 atggccagaa taccaaccaa aaaagctaaa gcaaaaaaag ggtttgaaga aacattatgg
     9121 gatgccgcaa atcagcttcg cggcagcgtt gagtcctccg aatacaagca cgtggtgttg
     9181 agcctcgtgt tcctgaaatt catcagcgat aagtttgaaa cacgccgcaa aaaaatgatt
     9241 gccgatgggc aggcagattt ccttgagatg gaagtgttct accagcagga caacattttc
     9301 tacctgccgg aagaggcgcg ttggtcattt atcaaacaaa atgcaaaaca ggacgatatt
     9361 gcggttcgta ttgacaccgc cctctcgacc attgagaaac gtaaccaaac cctgaaaggt
     9421 gcgctgccag acaactactt cagccgtcag aatctggaaa ccaaaaaact ggcatcactg
     9481 attgatacca tcgacaacat cgaaacgctg gcacacgaga ctgacgttga aacgttatcg
     9541 aaagaagacc tggtcggacg cgtttatgaa tacttcctcg gtaagtttgc cgccactgaa
     9601 ggcaaaggcg gtggtgagtt ctacacgcca aaatgtgtgg tcacgctgtt aactgaaatg
     9661 ctcgaaccct tccagggcaa aatttatgac ccgtgctgcg gctcggcagg aatgttcgtg
     9721 cagtcggtga agtttgtcga gagccatcag ggtaaaagcc gtgatatcgc gctgtatggt
     9781 caggagctga cagccacgac gtataaactg gcaaaaatga acctcgctat tcgcggtctt
     9841 tcagctaacc tcggcgaacg cccggcaaac actttcttta gcgaccagca cccggacctg
     9901 aaagctgact atattctggc gaacccgccg ttcaacctga aagactggcg taacgaagcc
     9961 gaattaacca aagacccacg ttttgccggt tatcgtatgc cgccaaccgg taacgccaac
    10021 tacggctgga ttttgcatat gctctccaag ctgtcggcta acggcacagc aggttttgtg
    10081 ctggcaaacg gttcgatgag ttctaacacc agcggtgaag gcgagatccg tgcacagatg
    10141 atcgaaaatg atctgatcga ctgcatgatt gctctgccag gtcagttgtt ttacaccacg
    10201 cagatcccgg tgtgtttatg gtttatgacc aaatcgaagg ctgccgatcc ggccaaaggt
    10261 tatcgtgatc gtcagggcga gacgctgttt attgatgcgc gtaacctcgg caccatgatt
    10321 agccgcacaa ctaaagagtt aacgacggaa gatattgcca caatcgccaa tacctaccat
    10381 gcatggcgca gcacacagga agaactggct gcacggattg cgcgtggtga cagcaagctg
    10441 gaaaaatatg aagaccaggc aggcttctgc aaagttgcaa ccctgcaaga cattaaagat
    10501 aacgactacg ttctgacacc gggccgctat gtaggagcag ccgagcagga agaggacggc
    10561 gtggcatttg agacgaaaat gcgtgaattg tcgaagacgt tgtttgagca gatgaagcag
    10621 gcggaagaac tggatcgtgc gattcgccag aatctggagg cgctgggtta tggggagtaa
    10681 tgctattaca acaataattg gtgaacactt gctgattaga aatggcaaat cttcgccatc
    10741 aagagccata acaggagaat atcctgttta tggttcaaat ggaataatag gttattcaga
    10801 tgaata
//
LOCUS       DAPHCV010000136        10730 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.137, whole genome shotgun sequence.
ACCESSION   DAPHCV010000136 DAPHCV010000000
VERSION     DAPHCV010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.137"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            100..813
                     /locus_tag="RH033_003991"
     CDS             100..813
                     /locus_tag="RH033_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDV2993105.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAATMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEILQASLRAIHAALS"
     gene            845..1603
                     /locus_tag="RH033_003992"
     CDS             845..1603
                     /locus_tag="RH033_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993106.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIDTAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1649..2998
                     /locus_tag="RH033_003993"
     CDS             1649..2998
                     /locus_tag="RH033_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HDV2993107.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYASNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            2998..3822
                     /locus_tag="RH033_003994"
     CDS             2998..3822
                     /locus_tag="RH033_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993108.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVNRQELLRLICKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRVYLSSPVWSLVNYSLRHSQ
                     LESVSGFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPMALDSDIRQRLELLISRFPL"
     gene            3834..4394
                     /locus_tag="RH033_003995"
     CDS             3834..4394
                     /locus_tag="RH033_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDV2993109.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLRNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWHLQEQSSTILVLNKTGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            4424..5494
                     /gene="lptF"
                     /locus_tag="RH033_003996"
     CDS             4424..5494
                     /gene="lptF"
                     /locus_tag="RH033_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDV2993110.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFVAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            5491..6570
                     /gene="lptG"
                     /locus_tag="RH033_003997"
     CDS             5491..6570
                     /gene="lptG"
                     /locus_tag="RH033_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDV2993111.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVDVEIFHYRDDLSLESYIYARSATIEDDKTWILHGVNHKKWLNGKETQ
                     ETSDNLPWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(6605..7777)
                     /locus_tag="RH033_003998"
     CDS             complement(6605..7777)
                     /locus_tag="RH033_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDV2993112.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIREGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDVN
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLVEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTISTQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(7777..8025)
                     /locus_tag="RH033_003999"
     CDS             complement(7777..8025)
                     /locus_tag="RH033_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDV2993113.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(8057..8971)
                     /locus_tag="RH033_004000"
     CDS             complement(8057..8971)
                     /locus_tag="RH033_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDV2993114.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRTLTRTARAHVNDNL
                     IWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLIAMADEITLGIFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLNAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAEARCGSVRMVGIPLPLLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SANNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(8968..10701)
                     /locus_tag="RH033_004001"
     CDS             complement(8968..10701)
                     /locus_tag="RH033_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HDV2993115.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRC
                     QLEDQLEYQTLKARAEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFSCQCAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNNPELHVLSHAW
                     FKALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHHEVMANLRAISHDGIKLRP
                     GDRCVSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSV
                     APPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTF
                     MPCYGLAENALAVSFSDEACGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKAL
                     PEHGIEIRNEADIPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYL
                     LDGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQC
                     RISDEERRGQLIHALVARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAY
                     AASLHVQESLA"
BASE COUNT         2593 a         2671 c         2799 g         2667 t
ORIGIN      
        1 ggttaatgtc acaattaacc attgtcacaa taaggttgaa cggatattct gagcacggac
       61 cacgtaatat tcaatacatt atttgctgcc gggtttttcg tgaatcaacg taatatgtca
      121 ataattaatt ccacgccagt gcgtgttatt gccattgtag gatgtgatgg ttcaggtaaa
      181 tcgaccctca cggcaagcct ggtaaatgaa ctggcagcaa caatgccaac agaacacatt
      241 tatctcgggc aatcgtccgg gcgaattggc gaatggattt cacagctccc tgttattggc
      301 gcaccttttg ggcgttatct gcgaagtaaa gcggcacatg tgcacgaaaa gccctccaca
      361 ccacctggca atattactgc gctggttatc tatctgcttt cctgctggcg ggcgtacaag
      421 tttcgcaaaa tgttgtgtaa aagccagcaa ggctatctgc tcatcaccga ccgctacccg
      481 caggttgaag tgccgggttt tcgctttgat ggcccgcaat tggcaaaaac cacgggcggt
      541 aacggttgga taaaaatgtt aaggcagcgc gagctgaagc tgtaccaatg gatggcgtct
      601 tatttgcccg tattgttgat tcgtcttggc attgatgaac aaaccgcgtt tgcgcgtaaa
      661 cctgaccacc aactggcggc gttacaggaa aaaatcgccg ttacgccgca actgacattt
      721 aatggcgcaa agatccttga actggatggt cggcaacccg ccgatgaaat tctgcaagcg
      781 tcactacgcg caattcacgc cgcgctttcc tgatcaccaa aatggatagg ggatgattta
      841 acgaatggac gcactacaaa cacaaactgt taatagtaca accgcgccgc agcccaacta
      901 cattccgggg ctgattgcgg tggtcgggtg tgatggcacc ggtaaatcca cactgaccac
      961 cgacctggtg aaatcgctgc aacaacactg gcaaaccgag cggcgctatc tggggctgct
     1021 ctcgggcgaa gacggcgaca aaatcaaacg attgccgttg gttggcgtct ggctcgaacg
     1081 gcgactggcg gccaaatcct cgaaaaccca aagcatgaaa accaaatctc cagcgctatg
     1141 ggcggcggtg attatgtact gcttctcgct gcgaagaatg gcgaatctac gcaaggttca
     1201 gcgactggcg caaagtggcg ttctggtggt cagcgatcgc ttcccgcagg ctgaaatttc
     1261 gggcttttat tatgacggac cggggatcgg cgtcgaacgt gcgaccggga aaatcagcat
     1321 gtttctggcg cagcgcgaac ggcgtttata ccaacaaatg gcgcaatacc gtcctgaatt
     1381 aatcattcgt ttggggattg atattgatac tgccatctcc cgcaagcctg accatgacta
     1441 tgccgagctg caggacaaaa tcggcgtcat gtcgacgatt ggctataacg gcacaaaaat
     1501 tcttgagatt gattccagag cgccctacag cgaagtgctg gaacaggcac agaaggcggt
     1561 ttctctggtt gccatcgttt ctgaccgccg aagtttaact taaggctggc gggatacttt
     1621 ttactatcaa cgataaggtc aggctgaatt ggcgggtttt aacatcaaac attggtttgc
     1681 agatggcgcg tttcgcacca ttattcgcaa tagcgcctgg ttaggctcca gtaatgtcgt
     1741 gagcgctttg ctgggtctgt tggcgctctc gtgtgccggt aaagggatga cgcccgccat
     1801 gtttggcgta ctggtgattg tacaatcgta cgccaagtcg atcagcgatt ttattaagtt
     1861 tcagacatgg cagctggtgg ttcagtacgg aacaccagca ttaaccaaca ataatccgca
     1921 gcaattccgc aatgtcgtct cattttcctt ctcgctggat atcgtcagcg gcgcggtggc
     1981 gattgtcggc ggcattgcct tactcccttt tctttcccat tcattaggtc tggatgacca
     2041 aagcttttgg ctggcagcgc tgtattgcac gctcattcct tcaatggctt cctccacgcc
     2101 gaccggcatt ctgcgtgcgg tagatcgctt cgatctaatt gctgtacagc aagcgacgaa
     2161 accttttctg cgcgcagcgg ggagcgtcgt tgcctggtat tttgactttg gttttgcggg
     2221 ttttgttatt gcctggtacg cctcgaatct tgttggcggc accatgtact ggtggtttgc
     2281 cgcacgtgaa ttacgccgca gaaatatcca taacgcgttc aaattgaatc tgtttgagtc
     2341 tgcccgacac atcaaaggcg cgtggagttt tgtctggtca accaacattg cccattccat
     2401 ctggtcggcg cgtaattcgt gcagcacagt gttggtgggg atcgtgttag gacccgctgc
     2461 cgccgggtta tttaaaatcg ccatgacatt cttcgacgcc gccggaacgc cagcgggttt
     2521 actgggcaaa agtttttacc cagaggtgat gcgtttagat ccgcgtacca ccagaccgtg
     2581 gttgttaggc gtgaagtcag gtttactggc gggcggaatc ggtattctgg tggcgctcgc
     2641 ggtgttgatt gtcggcaagc cgctcatttc gctggtgttt ggcgttaagt atctcgaagc
     2701 gtatgacctg attcaggtga tgctgggcgc aattgtgatc tcgatgctgg gcttccctca
     2761 ggagtcattg ctattgatgg cgggaaaaca gcgcgctttt ctcgtggcgc aaaccatcgc
     2821 ctcaatcggc tacatcgtat tgctgttcat gttctgtcat ctgtttggcg tgctgggagc
     2881 agcgtttgct tatttcggtg gtcaatgtct ggatgtggtg ctttcgctaa ttccgacctt
     2941 aaaggcattt tttcagcgcc attccttgct ttataacgca gccggagaga aatcctgatg
     3001 atgagaaaat atttcccgct agaggcgagc gagcgtctat ttgttgcgat tgaagaagat
     3061 gatgtcgttg atgcacaggt cagtttgccg ccgactatcg cactaagttg taccaccgaa
     3121 atcatccatg ataattacgc gttatgcctg caattctggc tcaatggcgt caatcgccag
     3181 gagttactgc gtttaatatg caaacaggcc aaaggcgatg agctgactgc tgatgagcgc
     3241 aaacaattta aatacatgcg agcgcgctac aagcacctgc gttttgcgca acggctgtat
     3301 ttaaagaagc atcaggccgg atttttattc ggcaaaacga ccgttttttt ggggcgtttt
     3361 caggacggct ttcgtaacgg taagaaaaat attgtgtcgt attacggcaa cctgttacgg
     3421 gtttatttaa gctctccggt ctggtcgttg gtgaactatt cattacgcca tagccagtta
     3481 gaaagcgtaa gcggttttat tgcctatcgg caaaagcaaa tgcataccct gaaagagata
     3541 attgccaaac cgcgactaac cggcagggaa tttcatgatg tgcgaaaaat catcagccag
     3601 caggtttctt attacgacac cctaagatcg cttgatcctg aaaataaaga agcgttacag
     3661 atttcccgct tcctcgccgc cattaatggc ctgatggggg ataaacacga cgacatggtg
     3721 gcggatgata tggaaaaccg ccagtcgtat gatgcaccga tggcgctgga tagcgatatt
     3781 cgccagcgct tagaactgct gatttcgcgt tttccgctat aaccggagca atgatgtcat
     3841 ctcgtttgat tattgcgctg attatcatgc tcttagcccc gggtgtgcag gcacacaatt
     3901 tcgtcactgg caaaacggtt acccccgttt atatccagga aggcggcgag ctgcttcgga
     3961 atagcgacga cgaaattcac taccaaaagt ggaacagcac ccaattggcg ggaaaagttc
     4021 gcattattca gtatatcgcc gggcgtaagt cggcgaagaa aaagaactca ctgttaatta
     4081 aagcagttga agcggcaaac tttccgcagg atcgttttca gcccaccacc attgtgaaca
     4141 ctgatgatgc catctttggt accggctatt ttgtcgttgg caaaatcgaa aaaaataaac
     4201 gccgctatcc atgggcgcaa ttcgttattg acggcaatgg acagggacgc gttgcctggc
     4261 acttgcagga gcagagttca accattctgg tgctgaataa aaccgggcaa attcagtggg
     4321 caaaggacgg cagcctgacg ccggaagagg tagatcatgt cattgcgctg gcgcagaagc
     4381 tgatcaatga ataaaaattc aaaggattga gggcgttagt gagatgaagc tggttgaaca
     4441 ctacatcatg cgtggtacgc gccgtctggt gctgattatc gtcggttttc tgatctttat
     4501 tttcgccagc tactccgcac agcgttatct gaccgaagcg gcaaatggga cattagctct
     4561 tgatgttgtg ctggatatcg ttttctacaa agtgctgatt gcactggaga tgttgttacc
     4621 tgttggactg tatgtgtcag ttggcgtgac gctagggcag atgtataccg actcggaaat
     4681 taccgctatc tctgcggcgg ggggcagtcc gggacgcttg tacaaagccg ttctttatct
     4741 ggcgataccg ctaagtattt ttgtcaccct tctgtcgatg tatggtcgtc cgtgggctta
     4801 tgcgcagatt tatcaactgg agcaacagtc acagtcggag ctggatgttc gccagttgcg
     4861 ggcaaagaaa tttaacacta acgataacgg acgaatgatc ctttcgcaga cggttgatca
     4921 ggataataat cgcctgactg atgcgctgat ttatacttct actgctaatc gaacccgcat
     4981 tttccgcgcc cgttcggttg atgtggttga cccatcacct gagaaaccga ccgttatgtt
     5041 gcataacggg accgcctatc ttctcgatca tcaggggcgt gacgacaacg aacagatcta
     5101 ccgtaatctg caattacatc tgaatccgct ggatcaaagc cctaacgtca aacgcaaagc
     5161 aaaatcggtc acggagctgg cgcgctccgc ctttcctgcc gatcatgccg aactgcaatg
     5221 gcgacaaagc cgtggcctga cagcattgtt gatggcgctg ctggccattt cattaagtcg
     5281 ggtaaaaccg cggcaagggc gattttcaac gttattgcca ctgacgttgc tgtttgttgc
     5341 cattttttat ggcggcgacg tctgccgtac gctggtggct aacggcgcga ttcccctcat
     5401 tcctggtttg tggttagtac ccggactcat gctaatgggc ctgctgatgt tggtcgcacg
     5461 cgacttctct ttgctgcaga aattttcccg atgaatgttt tcagtcgcta tttaatccgt
     5521 catctctttc tcggttttgc cgccgccgca gggctattgc tgccgctttt taccaccttc
     5581 aacctgatta acgaactgga tgatgtcagc ccgggtggtt atcgctggac tcaggcggtg
     5641 ctggtggtgc tgatgacctt accgcgcaca ctggtcgaac tttcgccgtt tatcgcctta
     5701 ttgggaggga ttgtcggcct ggggcagtta tcgaaaaaca gtgagcttac cgccattcgc
     5761 agcacggggt tttctatctt ccgtattgca ctggtggcgc tggttgccgg gatattgtgg
     5821 actgtttcat taggcgcgat agatgagtgg gtggcgtcgc cattgcagca acaggcgctg
     5881 caaatcaaat cgactgccac tgcgttgggg gaggacgatg acattaccgg caatatgctg
     5941 tgggccaggc gtggtaatga atttgtgacg gtgaaatcgc tgaacgagca gggccagccg
     6001 gtggacgtgg agatatttca ttaccgcgat gatctctcgc tcgaatccta catttatgcg
     6061 cgcagtgcta ccattgaaga cgacaaaacg tggatcctgc atggtgtgaa tcataaaaaa
     6121 tggctgaatg gcaaagaaac gcaggaaaca tctgataatc ttccctggca atcggccttc
     6181 accagtatga atcttgaaga gttatcgatg ccgggaaata ctttttctgt ccgtcaactt
     6241 aatcattaca tccattattt gcaggaaacc gggcaaccca gcagcgaata ccgccttgca
     6301 ctgtgggaaa aactgggaca acccatcttg accctggcga tgattttgct ggctgtgccg
     6361 tttaccttta gcgccccgcg ttcgcctggg atgggtagcc gtctcgctgt aggcgtcatc
     6421 gttggcttac tcacctggat cagctatcaa atcatggtca atctgggatt gttatttgcg
     6481 ttgagcgcac ctgttaccgc gctcggttta ccggtagcgt ttgtgctggt ggcgttgagc
     6541 ctggtgtatt ggtatgacag acaacattaa acccgccgtg gcgcaacacc acagcgggct
     6601 cacattactg cttcagagta gcaaatgcct gaatgatggc atcgatttgc tcacgactgt
     6661 gggcagcgtt tacgctacag cgcagcagcg tgatgcctgc aggcgcggca ggtggcagga
     6721 taagattcac ataaacgccc agcgaaataa gctcgcgcca gatgcgtaaa ccttcctctt
     6781 tcgaaccgat tattactggc actaccgggc taatatgtga acttaactca taacccagtt
     6841 tttgcagacc gtcatacaga tggttggcgt tatccatcag tttttgccgc agctctggtt
     6901 gtgtggaaat ggttcttaac gacgagcgca cggtcgcaat gcaggacgga gaaggagagg
     6961 cggtaaaaat atacgggcgg ctgccgtagc gcagcacttc catcgcttct gaacccacgg
     7021 caaaaccacc aatggaagcc aaacttttac tgaaggtgcc gacgataata tccacgtcgt
     7081 cttcaacgcc aacggcttca accagtccgc ggcctgttgc gcctaacacg ccaaacgaat
     7141 gtgcttcatc gacaatcaaa taaccaccga gacgacgttt gatatcaaca atttctgcca
     7201 gcggcgcaac gtcaccgagc atactgtaga tgccttcaac gataataatc gcctctttcg
     7261 cccgttcacc gaggcggacc atgcgccgtt ccagatcgtt gacgtcatta tggcggaaac
     7321 gaataatctc tgcgccgccg agagaacagg cgtcataaat actggcgtgg ctgtcggcat
     7381 ccagtaacac cacggcgtta tggtcggcaa gtgcgctgat gacacctaaa ttagcggtat
     7441 aaccggttga aaaaacgata gcggtagggc gattgaaaaa ctcggcaatc tctttctcta
     7501 acgccagatg cggcgcataa ctgccgtttg ccatacgaga accggtggtg ccagtgcctt
     7561 gggccgctaa cgctgcctga ccttcgcgga tagcatcatg attaaaggtt aaaccgagat
     7621 aattattggt cccggccaga atcacttttt tattgccgat ccggccttcg gtggcggaat
     7681 acacttcatc aatacaggtg ccaaatggtg ttaacccgtt gtcactaaat tgcaggcgct
     7741 cgccagcaag gcgcgcatat ttatcgtata gccccattat ttattctcca gccatggaag
     7801 tagagtttcc attaattgcg caggggtttt gacatccagc agaatattaa taggaatgga
     7861 aatatcgaat ctgtcttcca gcatcattaa gagatccatc actttgatcg actccaggcc
     7921 aagatcgttg acgagatcgc tgtcgggctt aatttccaca ccgttttcga ccaaatcctg
     7981 taaacaggaa aggatgtaat ccattactat ttcacgattt accatagaaa cgttaacaca
     8041 cctttaaaca agacaatcaa aatagcccgt tgcgtaatgc gtgttccagg ctaatcccgg
     8101 gaaaccaatt aatatcttca gaaatacggt tattatttgc cgaccagtcg gcgtgggtta
     8161 attcacgaat tttcgagcgg gtcagcatag gttctttacc tgccaggcga ctcaacgcag
     8221 tactgatatc cgcaaggcag gtgagtagcg gcagaggaat gccaaccatc cgcacagaac
     8281 cacaacgggc ttcggcggca agctgtcgta cgcgttgcca gtcatagccg ccagcgacgc
     8341 catcgcataa ttcataggtt tgcgtctgta cagtttcggc gtttaaccac tgacccactg
     8401 cttgcgcaaa atcggtgacg tgcaggaaag agagttgtgt atctggcgca ccaagtcgtg
     8461 gcagcaggcc gcgcagcatc cagtcaaaca gcggttttaa ctctttatcg ccgggaccat
     8521 acacggctgt cgggcgaaaa atgcccagcg taatttcgtc agccattgcg atcagtcgtt
     8581 gttcggcgac gtgtttggaa tttgcgtacc aggagagttc gggatggcgc gccgccagcg
     8641 aagagataaa cagaaaacgt tggcaaaagc cgctctcttt tgccgcttgc atcaggcgca
     8701 ggctgccgtc aacgttacag cgggtaaaaa tctcttcttt gtgtccgcgc acttgtccgg
     8761 cgcaatggac caccgcgctg gctccggcaa ccagttcgct aagcgaatgt gtatcttcca
     8821 gcgaaccgcg cacccagatg agattatcat tgacgtgagc gcgggcagta cgcgtcaatg
     8881 tgcgaacgtg aaagccgcgg gcgagcaggt tatcgataat atatttaccg ataaacccgg
     8941 tagcgcccgt caccgcgaca gtttgattca tgccagggat tcctgcacat gaaggcttgc
     9001 agcataagcc ttctgataac gttttttcgc ttccgcacgg gcaggcttgc cggaggacgt
     9061 tcggggaata ctgtggggcg gcaacagatc gatagccgcg gtcacgccaa attcgctttg
     9121 gatccgtgct accagtgcgt ggataagctg cccacgacgt tcttcgtcgc tgatccgaca
     9181 ctggatctgc aaaatgattt tttcctgggc ggtaacaaaa gcaatcgcat cgccagaatg
     9241 aatttccggt tcctgttccg caatgtattc aatatcctgc ggccagatat tacggccacg
     9301 aataataatc agatctttaa tgcgtccggt gacatacaga taaccgtcca gcagataacc
     9361 gaggtcgccg gtgtctaacc agcccgtcgc ggcaatctcg tcttgcgaaa cctggtcgcc
     9421 aaagtacccg ctcatcaggc tggggccgga gatgcaaata tggcctacca cacgttccgc
     9481 gacaggtata tctgcttcat tgcggatttc aatgccatgt tccggcaacg ctttaccgca
     9541 gttgacgaaa gtcgatacgg cgcgtgtttc agcacccggc gcgacggctt taccctggta
     9601 ttcgaggatg tcgcgatcca cttcgttaac cacaaccccg caggcttcat cagagaagct
     9661 gacagccagc gcattttctg ccagtccgta gcacggcatg aaagttttat cgtcaaagtt
     9721 aacctgacga aaacattcag cgaattgatg gagttgttct gcggagatcg gttctgctcc
     9781 aataccagcg acgcgccagc aggaaagatc cagttcagcg agatcttttt cattcacgcg
     9841 gcgctggcac aattcatagc caaacggcgg tgcaacggaa acggtgccgc gatttttact
     9901 gatcaattta agccattgca gaggacgcat ggcaaaatcc tgagtgcgca aataatctac
     9961 tgaaagctgc gtggcgacgg gggtcaggag aaagccgacc agtcccatat catggtagaa
    10021 aggcagccag gagacgcagc ggtcgccagg gcgtaattta atgccatcgt ggcttatagc
    10081 acgtagatta gccatcactt cgtgatgggt gataatgacg ccacggggaa aacgggtgct
    10141 gccggaggtg tactggaggt aggcgatatc gttcggaact ggacgctgga gcgcaacatc
    10201 ggcttccggt aatgccttaa accaggcgtg gcttaaaaca tgtaattcgg ggttgttgtt
    10261 atgcgtcgcg gcattgacca gcggtaacca ctcatcacca gtgataatgg ctgcgggctg
    10321 gcaacttgcc agtaaaccct gcagtttggc gctccaggaa tcccgctgac cgacgcccat
    10381 tggaatcgcc aacgggacgg cgactaagcc ggcacactgg caggaaaaaa aagcctctac
    10441 aaacccgcta ctcgtttcgg caatcagcgc cacgcgatcg ccttttttca ggttcagcga
    10501 taacaaccgc ttcgcaccag cttcggcacg cgcttttaac gtctgatatt ccagttgatc
    10561 ttcaagttgg caacgtctgt cataaaaatt cattccggcg ctactcagag cggcgtagtc
    10621 caaagcatca accagcgttg gaaaatcggc atagcgcata ggtagggaat gcgtaaagat
    10681 tttattagac atatacacca cgaaaaaatt ggatatcaaa aataaaatga
//
LOCUS       DAPHCV010000137        10611 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.138, whole genome shotgun sequence.
ACCESSION   DAPHCV010000137 DAPHCV010000000
VERSION     DAPHCV010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.138"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            168..1535
                     /gene="dcuC"
                     /locus_tag="RH033_004002"
     CDS             168..1535
                     /gene="dcuC"
                     /locus_tag="RH033_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HDV2993116.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCIIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKALDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTLFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(1579..2076)
                     /gene="sspB"
                     /locus_tag="RH033_004003"
     CDS             complement(1579..2076)
                     /gene="sspB"
                     /locus_tag="RH033_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HDV2993117.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDASIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(2082..2720)
                     /gene="sspA"
                     /locus_tag="RH033_004004"
     CDS             complement(2082..2720)
                     /gene="sspA"
                     /locus_tag="RH033_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HDV2993118.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(3115..3507)
                     /gene="rpsI"
                     /locus_tag="RH033_004005"
     CDS             complement(3115..3507)
                     /gene="rpsI"
                     /locus_tag="RH033_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDV2993119.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3523..3951)
                     /gene="rplM"
                     /locus_tag="RH033_004006"
     CDS             complement(3523..3951)
                     /gene="rplM"
                     /locus_tag="RH033_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDV2993120.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4170..5297)
                     /gene="zapE"
                     /locus_tag="RH033_004007"
     CDS             complement(4170..5297)
                     /gene="zapE"
                     /locus_tag="RH033_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HDV2993121.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYDIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5491..5889
                     /locus_tag="RH033_004008"
     CDS             5491..5889
                     /locus_tag="RH033_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HDV2993122.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            6043..7410
                     /gene="degQ"
                     /locus_tag="RH033_004009"
     CDS             6043..7410
                     /gene="degQ"
                     /locus_tag="RH033_004009"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HDV2993123.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGIKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            7500..8567
                     /gene="degS"
                     /locus_tag="RH033_004010"
     CDS             7500..8567
                     /gene="degS"
                     /locus_tag="RH033_004010"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HDV2993124.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINPRRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGM
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVNNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8630..9568)
                     /gene="mdh"
                     /locus_tag="RH033_004011"
     CDS             complement(8630..9568)
                     /gene="mdh"
                     /locus_tag="RH033_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDV2993125.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            10003..10473
                     /gene="argR"
                     /locus_tag="RH033_004012"
     CDS             10003..10473
                     /gene="argR"
                     /locus_tag="RH033_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HDV2993126.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2607 a         2693 c         2644 g         2667 t
ORIGIN      
        1 ttcttacaaa ataatcataa gcgcatattt tttaatgaaa aatcacctca tctacaatta
       61 aaaacacgac atccgcacca taaatagcct tgcaaaaaat ataacatcat tgttttcaat
      121 ctgccgttta tgggattgac cattttcttt tgacacggag ttcaacaatg ttcggcataa
      181 ttatatctgt catcgtatta attacgatgg gctatttgat cctgaaaaac tacaaacctc
      241 aggtggtgct ggctgccgca ggtatcttcc tgatgatgtg cggtgtctgg ttagggttcg
      301 gtggtgtgct cgatcccgcc aaaagcagcg gctacttgat cgtcgatatt tataatgaaa
      361 tcctgcgcat gctgtccaac cgcattgctg gattggggct gtcgattatg gcagtgggcg
      421 gttatgcccg ctatatggag cgcatagggg caagtcgcgc gatggtgagc ttgttaagcc
      481 gcccgttaaa actcattcgc tcgccgtata ttattctgtc ggcaacttac gtcatcggcc
      541 aaatcatggc gcagtttatt accagcgcct ccggtctggg tatgttgctg atggtcacct
      601 tatttccgac gctggtgagt ctgggagtaa gtcgtctctc tgcggtggcg gttatcgcaa
      661 ccaccatgtc cattgagtgg gggattctgg aaacgaactc catttttgct gcccaggtcg
      721 caggaatgaa aattgccact tacttcttcc actaccagct tccggtcgcc tcttgcatca
      781 ttatctcggt ggcgatctcc cactttttcg tgcaacgcgc tttcgacaaa aaagataaaa
      841 atatcaatca cgaacaggca gagcaaaaag ctctcgataa tgtcccgccg ctctattacg
      901 ccattttacc ggtgatgccg ttaatcctga tgctcggctc gctgttcctt gcccacgtcg
      961 ggctgatgca gtcagaactg catctggtgg tggtgatgtt actgagtttg acggtgacgc
     1021 tgtttgttga gttcttccgc aagcataact tgcgcgaaac aatggacgat gtgcaggcgt
     1081 tttttgacgg catgggtacg cagtttgcca acgtggtaac gctggtggtc gcgggtgaaa
     1141 tatttgcgaa aggcttaacg acgattggca ctgtcgatgc ggttatcagg ggggcggagc
     1201 attctggtct gggcggtatt ggcgtgatga ttattatggc gctggtcatt gccatttgtg
     1261 ccattgtgat gggctcaggc aatgcgccgt ttatgtcatt tgccagtctt attccgaata
     1321 tcgcagccgg actacatgta ccagcggttg taatgattat gccgatgcat tttgccacga
     1381 cgctagcgcg cgctgtttcg ccgattactg cggtggtggt cgttacgtca ggaattgcag
     1441 gcgtttcgcc ttttgcggta gtgaagcgga cagcgatccc catggcagtc ggtttcgtgg
     1501 tgaatatgat tgccactatc acgctatttt attaagcgat taaaaagaca aaacaggccg
     1561 ccagggcctg tttttgtatt acttcacaac gcgtaatgcc ggtcgaccac cgcgtggtgg
     1621 ctgcggtggt tcatcgtcag gatgagtgtc atcatcgtga tctggcttgt cgccatcaat
     1681 aaccgacata acggtttcgt tgtctgccga tgcctcttca tcattcatga tgctggcatc
     1741 ttcatcgtag gcagcttcag gttcaaacat cgtgcctgcg ccattttcac gggcgtagat
     1801 agccagcacg gcagccagcg gcacagaaac ctgacgcgga atgccaccaa agcgcgcgtt
     1861 aaagcgcacc tcatcattcg ccagttccag attgccgaca gctcgcggcg caatgttgag
     1921 tacgatttgc ccgtcacgcg catattccat aggaacctgc acgccaggga gcgtcacatc
     1981 caccaccagg tgcggcgtga gctggttatc cagcaaccac tcatagaatg cacgcagcag
     2041 atagggacga cgtggtgtta gctgtgacaa atccatacag attaactccg gcccagacgc
     2101 atttcacgtt cagcttcagt taaagaagca aggaaagagt cacgctcaaa gacgcgggtc
     2161 atatagcctt tcagctcttt cgcacccggg ccgctgaact cgatgcccag ttgcggcaga
     2221 cgccacagca gaggagcaag gtagcaatcg accaggctga actcatcgct caggaagtac
     2281 ggcttctgac cgaagaccgg cgcaatcgcc agcagttctt cgcgcagttg cttacgtgcg
     2341 gcatcggctt cagaagctga accgttgatg atggtgttca tcagcgtgta ccagtctttt
     2401 tcgatgcgat gcatgtacag acggctttca ccgcgagcta ccgggtaaac aggcatcagt
     2461 ggcggatgcg ggaaacgctc atccagatat tccataatga tgcgagattc ccacagggtc
     2521 agctcacgat ccaccagggt cggaacgctc tgattcgggt tgaggtcaat cagatcctga
     2581 ggcggattgt ccttttccac gtgttcgatc tcgaaactta cacctttctc agccagcaca
     2641 atgcggacct gatggctata gatgtcagta ggaccggaaa acagcgtcat taccgaacgt
     2701 ttgttggcag cgacagccat gaaaacctcc aggtatagtc agaattttta ctgctaccag
     2761 ccaccaggtg gccagtcaga agttgtgtta cccagtaagg aacgactctc tttgttcgaa
     2821 aatcaaacaa aaaatgagca atacccgaca tttgggcaga aaattggatg atagtttacc
     2881 agattttgcg acctttgtgg tgagtcgatg ccggaaatgg ggaaaaagag atgcgcttta
     2941 gtctgaaata gttgacttag tcccttattg gcgatgtggt ttttgtttta cctgtctgtc
     3001 aggtggcagc aaaaagcaac ttcccatttt ttacgctgat gcagatttta gccataaaaa
     3061 aacccgccga agcgggtttt ttcgcaaatt gttttctgcc ggagcagaag ccaattaacg
     3121 tttggagaac tgcggacgac gacgtgcttt acgcagaccg actttcttac gttcaacctg
     3181 acgagcgtca cgagtaacga agccagcttt acgcagttca gaacgcaggg actcgtcgta
     3241 ttccatcaga gcgcgggtga taccgtgacg gatcgcacca gcctgaccag agataccacc
     3301 acctttaacg gtgatgtaca ggtccagttt ctcaaccatg tcgaccagtt ccagcggctg
     3361 acgaactacc atgcgagcag tttcacgacc aaagtactgt tccagagaac gttggttgat
     3421 tacgatttta ccgttgcccg gtttgatgaa aacgcgagct gcggaacttt tgcggcgacc
     3481 agtgccgtag tattgatttt cagccattgc ctataatccc gattagatgt caagaacttg
     3541 cggttgctgt gccgcgtggt tgtgctcgtt acccgcgtaa actttcagtt tacggaacat
     3601 agcacgaccc agcgggcctt ttggcaacat gcctttaacc gcgatttcaa tcacacgctc
     3661 aggacggcga gcaatcatct cttcaaaggt cgcttgtttg ataccaccga tgtggccggt
     3721 gtggtgatag tacactttgt cagtacgctt gttgccggtt acagcaactt tgtcagcgtt
     3781 cagaacgatg atgtaatcac cggtatctac gtgcggagtg tattccgctt tgtgcttacc
     3841 gcgcaggcga cgagccagtt cagtagccag acggcccaga gttttaccgg tcgcgtcaac
     3901 aacataccag tcgcgtttta cggtttctgg tttagctgta aaagttttca ttaaaagctt
     3961 acccaataaa aagttacacg ttggtgaaca cccaaacgtc ttcaattgtt gaggttcaca
     4021 cgacaaagtc cggcaaacct accccttcga atagcctatg ccagcacaca aaaagttttg
     4081 ggaaaaaaac tttcttgtaa cgtggggtcg caggattata gagaagtcgg ggtcaaagat
     4141 cgaccccttt ttgtgatttg tggcaggttt taacccgcca aatgctcgcg cttcagatac
     4201 tcttcgcttt gcatctcttg cagacgtgac aggcaacgct ggaactcaaa tttcagccgc
     4261 tcgccctgat aaatgtcata tagcggcact tctgcactca ccactaattt gacatggcgc
     4321 tcgtaaaact catccaccag cgcaataaaa cgtcgcgctt cgctctccat caaccgcgtc
     4381 ataactggta catcaaacaa catgactgtg tgaaagaggc gtgagagcgc aatatagtca
     4441 tgctgactgc gggcgtcgac gcacagcgta gtaaaagaga ccgccagcgt ctggttctcg
     4501 acgcccattg tcgccagcgg ccgatggttg atttctaatg tcggtgaatt ttctcgtttc
     4561 gcccccgcca gcgccaacca tagtttatcc atttgcgccc gggtttcatc gttgaggggc
     4621 gaaagccaca gatgcgcctg agtgagtgta cgcagtcgat aatcaacacc agcgtccacg
     4681 ttcattacat cacaatgctg tttaatggca tcgattgcag gcaaaaaacg cgcacgttgc
     4741 aggccatttc gataaagttc gtccggcgga atatttgacg tcgctaccag ggtaataccg
     4801 cgggcgaaca gggctttcat cagaccgcca agtagcatgg catcggtaat atcagaaaca
     4861 aaaaattcgt caaaacagag cacgtcagtt tctgctttaa agcgatcggc aataatttcc
     4921 agcggatcgg tctgcccctg caaagcagtt agctcttcat gtacccgcag cataaaacgg
     4981 tgaaagtgca gacgctgttt ccgctctccc ggcaggcttt gatagaaaag gtccatcagc
     5041 caggttttcc cgcgtcctac accgccccac atatataagc cacgcactgg cgtatgcttt
     5101 gtgtcttcgc gtttacccca cagcttaccg acccgcgcca ttagcccact cgtcctgggg
     5161 gctggaggcg tgctattgat gagttcctga taaataattt ccaggcggct gacggcctct
     5221 ttttgaacgt cgtcgggttg atggctgcct tcattgagcg ccttcaggta ttgcgatgtt
     5281 ggggtaacgc tttgcatgat cttattgtta ttccttgaat aatcggtgcg ccgttgttca
     5341 cggttgacga aaaaaaggcc gttctacact acgcgatatg cagtcgggat tccacttctg
     5401 tggaattaac ggttatagtg gcataatcag ccgcaggcat ggagcctgaa gccaacaccc
     5461 tacggaaaca aaagacaacg ggagatgttc atgacctggg aatatgcgct aattgggtta
     5521 gtcgtcggca tcattattgg tgctgtggcc atgcgttttg gtaatcgtaa actacgccag
     5581 caacaggcgt tgcagtacga actggaaaag aataaagctg aactggacga gtatcgcgaa
     5641 gaactggtta gccactttgc ccgcagcgcg gaattactgg ataccatggc gcacgactat
     5701 cgccagctgt atcagcacat ggcgaaaagc tccagtagcc tgctgccgga actgtctgcg
     5761 gaagcaaacc cgttccgtaa tcgtctggca gagtctgaag ccagcaacga tcaggcaccg
     5821 gtgcaaatgc ctcgcgacta ttctgaaggc gcatccggcc tgctgcgtac tggcgcgaag
     5881 cgcgactaat ttatttttcg ggcgcagcca ttgcgccctc ctcttctctc cctccccgac
     5941 tatcatttaa tctggtgtct cattgttagc cgtctgaaaa ttcaataaca tcaaactgtt
     6001 ttgaatctct tttcttatca ttcaggtacg agagcaggaa taatgaaaaa acaaacccag
     6061 ctgttgagtg cattagcgtt aagtgtcggg ttaactctct cggcgtcatt tcaggccgtt
     6121 gcgtcgattc caggccaggt tgccgatcag gcccctctcc ccagtctggc cccaatgctg
     6181 gaaaaagtgc ttccggcagt ggtgagcgta cgggtggaag gaacggccag tcagggacag
     6241 aaaatcccgg aagaattcaa aaagtttttt ggtgatgatt taccggatca acctgcacaa
     6301 cccttcgaag gtttaggctc cggtgtcatc atcaacgcca gtaaaggcta tgtgctgacc
     6361 aacaaccatg tgattaatca ggcacagaaa atcagtattc agctcaatga tgggcgcgag
     6421 tttgatgcaa aactgattgg tagcgatgac cagagcgata tcgccctgtt gcaaattcaa
     6481 aacccgagca aattaacgca aatcgctatt gccgactccg ataaattgcg cgtcggtgat
     6541 tttgccgtgg cggtcggtaa cccatttggc cttgggcaaa ccgctacctc tggcattgtg
     6601 tcagcattag gccgcagtgg gttgaatctt gaaggtctgg aaaactttat ccagacagat
     6661 gcttccatta accgcggtaa ctccggcggt gcactgttaa accttaacgg tgagttaatt
     6721 ggcatcaaca cggcaatcct tgcgcctggc ggtgggagcg tcgggattgg atttgccatc
     6781 cccagtaata tggcgcgaac actggcgcag cagcttatcg atttcggtga aatcaaacgc
     6841 ggtctgttag gcatcaaagg caccgagatg agtgccgata tcgccaaagc cttcaatctt
     6901 gacgtgcagc gtggcgcgtt tgtcagcgaa gtgttgccag gttctggctc ggcaaaagcg
     6961 ggcatcaaag cgggcgatat tattaccagc ctcaacggca aaccgctgaa tagctttgct
     7021 gagttgcgct ctcgtatcgc aaccaccgag ccgggcacga aagtgaagct tggcctgctg
     7081 cgtaacggca aaccactgga agtagaagtg acgctcgata ccagcacctc ttcgtcggcc
     7141 agcgctgaaa tgatcacgcc agcgcttgaa ggtgcaacgt tgagcgatgg tcagctaaaa
     7201 gatggcggca aaggtattaa gatcgatgag gttgtcaaag gaagcccagc tgctcaggca
     7261 ggcttgcaaa aagacgatgt gatcattggc gtcaaccgcg atcgggtgaa ctcgattgct
     7321 gaaatgcgta aagtgctggc ggcaaaaccg gccatcatcg ccctgcaaat tgtacgcggc
     7381 aatgaaagca tctatctgct gatgcgttaa tgtcgtaaac cgggcatcag gctgacgtgt
     7441 gatgtccggt taactcgtgg tatgctgctg ccgttccctt ttttaacgat gcctccatca
     7501 tgtttgtgaa gctcttacgt tccgttgcga ttggattaat tgtcggcgct attttgctgg
     7561 ttgccatgcc ttcgctgcgc agccttaacc cgctttccac tccgcaattt gacagtaccg
     7621 atgagacgcc tgccagctat aatctggcgg ttcgccgcgc cgcgccagcg gtggttaacg
     7681 tttacaaccg tggtttgaac accaactctc acaaccagct tgagatccgc accctgggat
     7741 ccggtgtaat catggatcaa cgcgggtata tcatcaccaa taaacacgtg atcaacgacg
     7801 ccgatcagat catcgtcgcc ttacaggatg gacgtgtttt tgaagcgtta ctggttggct
     7861 ctgactctct aaccgatctg gcggtgctga aaattaacgc cacaggcggt ttaccaacga
     7921 ttccgatcaa tccacgccgc gtaccgcaca ttggcgatgt ggttctggcg atcggtaacc
     7981 cgtacaacct cgggcagacc attacccagg ggattatcag tgccacgggt cgaatcggtc
     8041 tgaacccgac cgggcggcaa aacttcctgc aaaccgatgc ttccattaac cacggtaact
     8101 ctggcggcgc gctggtgaac tcgctgggcg aactgatggg cattaacacg ctgtcgtttg
     8161 ataagagtaa cgatggcgaa acgccggaag gtatcggctt tgcgattcct ttccagttag
     8221 cgaccaaaat tatggataaa ctgatccgtg atggccgtgt gatccgtggc tacattggca
     8281 ttggtggacg tgagatcgcc ccattacatg cgcagagtgg cggcatggat caactgcaag
     8341 ggatcgtggt taacgaagtg tcgcctgatg gcccagcggc aaatgcaggc atccaggtca
     8401 acgatctgat tatctctgtg aataacaaac ccgctatttc cgccctggag acaatggatc
     8461 aggtcgcgga aattcgccct ggttcggtga tcccggttgt cgtgatgcgt gacgataaac
     8521 agttaacatt gcaggtcacc attcaggaat atccggcaac caattaagtc gtgcgctcaa
     8581 aacaaaaaac cggagtctgt gctccggttt tttattatcc gctaatcaat tacttattaa
     8641 cgaactcttc gcccagggcg atatctttct tcagcgtatc cagcatacct tccagcgcgt
     8701 tctgttcaaa tgcgctcagg gtaccgatag atttacgctc ttccacgccg tttttaccca
     8761 gcagcagcgg ttgagagaag aaacgtgcgt actgaccgtc gccttcaacg taggcacatt
     8821 cgacaacgcc ttgttcgccc tgcagtgcgc gaaccagaga cagaccaaaa cgtgcagctg
     8881 cctggcccat agacagggtt gcagacccgc caccggcttt cgcttcaacc acctcagtac
     8941 ccgcgttctg gatacgtttg gtcagatcag ccacttcctg ctcggtaaag ctaacgccag
     9001 gaacctgtga cagcagcggc agaatggtaa caccagagtg accgccaata accggcactt
     9061 caacttcgcc tggctgtttg cctttcagtt ccgcaacaaa ggtgttggaa cgaatgatat
     9121 ccagcgtggt aacgccgaac agtttgtttt tgtcataaac accggctttt ttcagcactt
     9181 cagcagcaat cgcaactgtg gtgttaaccg ggttagtgat aataccaatg cacgctttcg
     9241 ggcaggtttt cgcaacttgc tgtaccaggt ttttcacgat gccggcgtta acgttaaaca
     9301 ggtcggaacg atccataccc ggtttacgcg ctacacctgc agagataaga acgacatctg
     9361 cgccttccag cgccggagtc gcatcttcac cagaaaaacc tttgattttc acagcagtag
     9421 ggatatggct cagatcgaca gccacaccgg gagtcactgg agcgatatca tacagagaga
     9481 gttctgaacc tgaaggcagt tgggttttta acagtagtgc aagcgcctgg ccaataccgc
     9541 cagcagcgcc gaggactgcg actttcatcc taaactcctt attatattga taaactaaga
     9601 tatgttgctc cgctgccgcg accttaatcc acaaaattgc cgtttacaat gaccacatct
     9661 caagaatgtg tagtcacgca agtttagcgt ttatgcattt aattgccgta atcaggaacc
     9721 taacttacgt aattaacagt cgcttatgca ttagcgcaac attccaacag gtggtgacaa
     9781 tataccctac cgttcagcca aaacaacatc aatttgataa caattaattt acttttaagc
     9841 agaatttgca tgccgtgacg caggcatgtt tctcaataac gaaatttgat aaaatcccgc
     9901 tctttcataa cattatttca gccttcttca gggctgactg tttgcataaa aattcatctg
     9961 tatgcacaat aatgttgtat caaccaccat atcgggtgac ttatgcgaag ctcggctaag
    10021 caagaagaac tagttaaagc atttaaagca ttacttaaag aagagaaatt tagctcccag
    10081 ggcgaaatcg tcgccgcatt gcaggagcaa ggctttgaca atattaatca gtctaaagtc
    10141 tcgcggatgt tgaccaagtt tggtgctgta cgtacacgca atgccaaaat ggaaatggtt
    10201 tactgcctgc cagctgaact gggtgtacca accacctcca gtccattgaa gaatctggtg
    10261 ctggatatcg actacaacga tgcagttgtc gtgattcata ccagccctgg cgcggcgcag
    10321 ttaattgctc gcctgctgga ctcactgggc aaagcagaag gtattctggg caccatcgct
    10381 ggcgatgaca ccatctttac tacccctgct aacggtttca ccgtcaaaga cctgtacgaa
    10441 gcgattttag agctgttcga ccaggagctt taatctctgc cccgtcgctt ctggcggcgg
    10501 ggaaaatgtt gcttttccct ctcacccccc ctgctttctc ctgcgattaa tttaacgaat
    10561 agtgcgtttt atcgtgacat attattcaca taatgaatac atgggttaaa a
//
LOCUS       DAPHCV010000138        10414 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.139, whole genome shotgun sequence.
ACCESSION   DAPHCV010000138 DAPHCV010000000
VERSION     DAPHCV010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.139"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1697)
                     /locus_tag="RH033_004013"
     CDS             complement(<1..1697)
                     /locus_tag="RH033_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993127.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQEIYERPYNSFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNNGPMQLEVPRDQYAGAVETMRNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     "
     gene            complement(1818..4937)
                     /gene="ag43"
                     /locus_tag="RH033_004014"
     CDS             complement(1818..4937)
                     /gene="ag43"
                     /locus_tag="RH033_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HDV2993128.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARARGKRGGVAVALSLAA
                     VTSLPVLAAYIVVHPGETVNGGTLANHDNQIVFGTTNGMTISTGLEYGPDNEANTGGQ
                     WVQDGGTANKTTVTSGGLQRVNPGGSVSDTVISAGGGQSLQGRAVNTTLNGGEQWMHE
                     GAIATGTVINDKGWQVVKPGTVATDTVVNTGAEGGPDAENGDTGQFVRGDAVRTTINK
                     NGRQIVRAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASDTVVNSDGWQ
                     IVKNGGVAGNTTVNQKGRLQVDAGGTATNVTLKQGGALVTSTAATVTGINRLGAFSVV
                     EGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADSG
                     AAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLAG
                     TTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGSLTGNGSVEKSGSGTLTVSNTTLT
                     QKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNIP
                     DNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNNA
                     DRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFVQGNRLQ
                     AGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRIVAGSRSHQTGVNGE
                     NNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLMRTEVAGMSVTAGVYG
                     AAGHSSVDVKDDDGSRVGTVRDDAGSLGGYLNLIHNASGLWADIVALGTRHSMKASTD
                     NNDFRARGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGKDNAGYVKFGHGS
                     AQHVRAGFRLGSHNDMTFGEGTSSRAPLRDSAKHSVSELPVNWWVQPSVIRTFSSRGD
                     MRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYAHSVSGSSAEGYN
                     GQATLNVTF"
     gene            complement(5265..6137)
                     /locus_tag="RH033_004015"
     CDS             complement(5265..6137)
                     /locus_tag="RH033_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HDV2993129.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLHKLTRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQRVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
     gene            7368..8852
                     /locus_tag="RH033_004016"
     CDS             7368..8852
                     /locus_tag="RH033_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HDV2993130.1"
                     /translation="MSFGQPCDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFFLTLAESGERKTAVDKLLMKPLYQQEMQLYSRYK
                     SELAVWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEVLQKNSAEEPVRYKFI
                     FNDATTAAIKNQLCGKWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VIPGGSLTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEHYNVLEQDLSPSG
                     PLSPFRGHVSKKTENIARIAALFQYFSYGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQQKDAEELFDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNALLNILES
                     QFRLSVVPEGKTMYVLLPQIASLKLSDVSGIFTSGYHYNKLRAK"
     gene            complement(9174..9776)
                     /locus_tag="RH033_004017"
     CDS             complement(9174..9776)
                     /locus_tag="RH033_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993131.1"
                     /translation="MKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRTTPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLLAYRTTVDDKRT"
     gene            complement(9863..10069)
                     /locus_tag="RH033_004018"
     CDS             complement(9863..10069)
                     /locus_tag="RH033_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDV2993132.1"
                     /translation="MLTPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGDFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
BASE COUNT         2410 a         2635 c         2725 g         2644 t
ORIGIN      
        1 gatgccgtca gggcaaaact gatccctccg gccgccagac tgacaaccga accctcggca
       61 atatcataag tgaccgattc gatggtcccg aaccgggtga tattacgggc ctgagtataa
      121 gtcagatgtc cccgacgaat cagccgggac gcttctgcgg gatcggttac accgggtact
      181 ttaccttcgc ggatcttatt cctcatggtt tcaacagcgc cggcatactg atcacggggg
      241 acttccagtt gcatggggcc attatttccc atgtaacgat actgtccgtt ttgtccgtcg
      301 aatgccgcac cgacgctacg ggctgcagtt gaacaatatt tcgtctggat ttctgttccg
      361 ctgaccagtc tgtctgctcc atttctggca ttattatcac cgaggatccg tgcgtttttc
      421 agacggactc tgtcgacaat atggtttccc cgttcagcgg caaacccatg tccctgccct
      481 ccggaagcaa acattttctc atttgtactc caggtttccg gcagtttcat gagaatcaga
      541 ccattcgccg ttgcatcgga aataaagctg ttgtaagggc gttcataaat ttcctgacac
      601 agtgtttctg tttcgcgaca aagagaggag cagtaccatt ccggatccgg cttatgattt
      661 tccggggcat gatgtacttc tgtcagcccg caggaggcac agatacgaag ctcatccacg
      721 tgatggaatg atacaaaacg gcgaccttct gaaaagggat accagtcacc gccgtccagg
      781 caacgatcga gaaaatcgtc attcgcttcc ggtgataaat tcaggcggtt ccggaagagc
      841 gttttcagtt catcaggtgt gaatgacagg ttctctttca actgttcgtt aaaccctgcc
      901 atgtttcttt cataccaggc attgttcagt aaccagtttc cgtctgccag ttcaaattcc
      961 ctcagctctc cccgggaaat ccgttgagaa atgagcgtca gccagatgcg ctccgtcagt
     1021 gagtcaggaa ggctgtaatc gttttgcaaa ctggtcagcc acgatttact gttgagcacc
     1081 ggttgttctt ccagtgtctg gctgatggca caggtcagtt ttttcagcgc ctggatctgg
     1141 ctggccgccc atagcagcgt cactaatgca gccagccagt acaacaaccc gccccaccac
     1201 atcagtgacg gtgaaatatc gtactgtaac gcgatcaatg gtgcgcatgc agaacacgcc
     1261 ataaggaaaa aggcagtata aaaacagcag taatacccgt taatttcctt gctgatatct
     1321 tctggtgtga tcccgttctg ctgaatgttt ttgatctgca ggggaacagc cagcagacca
     1381 ccggcgagag cggtggtcag aataaatttc acagagtcaa aggtgtgagc ctccagcgcg
     1441 atatgtttac catcaagtcc ccacatgaag ctgatgatca ttccggcaat cgtcagaaat
     1501 acgcctgcca gccagcgaat atggaacaac cgggttatgc gttcggtgat atagccagcc
     1561 agtaaggccg ggccgaacag acacagcaat atgagccctg ccagcgcaga agccgtgctg
     1621 attaatgctc tgaacaaaag gcgaagaatg gcaaatcctg cgatcagcag gatcagcgcg
     1681 ccgactatct gtaacatagt cactccgtga tgaatatcat gttattgtta atgccagcga
     1741 atgtggcgtt gaagatccgt tgcgccggtc cgggtcccgg tcatgatgac cgggaccaca
     1801 gagaggcgat ggttctgtca gaaggtcaca ttcagtgtgg cctgaccgtt atacccttca
     1861 gcgctgctgc cgctgacgct gtgggcataa ccggcctgaa cgcccagggt gatattttcc
     1921 cggacacggg cttccagtcc ggcctgcagg tccagtgatg tgccattctg tgagggagag
     1981 aacgtcatcc cgctgcctgc agtggaagtc cccacacgca tatctccccg ggagctgaag
     2041 gtgcggataa cagaaggctg tacccaccag ttcaccggta attcactcac actgtgtttt
     2101 gcactgtcac gcaggggggc acgggatgag gtgccttcgc caaaggtcat atcgttgtgg
     2161 ctgcccagac ggaaaccggc acgcacatgt tgtgcactgc catgcccgaa cttcacataa
     2221 ccggcgttgt ccttaccgtc atccagggaa agtccctgcc aggtatactg cagttgtggc
     2281 tccagcatca ggttgtcagt gatactgaag ggcagaccgg tttccagtga acccagccag
     2341 ccccaacccc gggcgcggaa gtcgttattg tccgttgacg ctttcatgct gtggcgggtt
     2401 cccagggcca caatgtcagc ccacagtccg gaggcgttgt gtatcagatt caggtatccg
     2461 cccaggctgc cggcatcatc ccggaccgtg ccgacacggg agccgtcatc atccttaaca
     2521 tcaacggaag aatggccagc agcaccatat acccccgcgg tcacagacat accggcaacc
     2581 tctgttctca tcaggtcacc ctccagacgg acgaatccat agctgccgct gctttccggc
     2641 gtggccccac gggcaatacc gccattgtta tcgtgaccga gatgaccgcc ctgaatgctg
     2701 agacggacgc tgttgttttc accatttaca ccggtctgat ggctgcggga gcctgccaca
     2761 atccggtcat agtccattgc ctgtgtcagc atggaggcat acagggggac ttctgcacga
     2821 taagcatttt cactgcgcag ataccagctc tcatcactgt cccggttgag ggagtagtta
     2881 aaggcaccgg cctgcagcct gttcccctgg acaaaggccc cttcctccgt ggtggcaccg
     2941 ttaatggctt caaccacctg aataccctta ccgctggtcg ccagccccga cgcactgttg
     3001 ccggcgttca ccaggttcag gatggttttt ccggttgccc tgccgccgtc aatgaccagt
     3061 ctgtcagcat tgttctgtgc catatccggg cgtacacgca ggctgatggt gccattctgt
     3121 ccgttcaggt ttttcacttt cagggttgcc ggtacgaact tccctgtgcg ggtggaggtg
     3181 aaatgaatct gtccggcatg gctgaggtca tccaccaccg actgcaccgt ggcgttatcg
     3241 gggatattcc aggtggcacc ggaggcgaga gtgacattcg tggggtcaat ggcaccgttc
     3301 agcacagtgc tgccggtcag cttcagggct gtaccgcgct gagcaatgac atccgtggtg
     3361 acggtactgt cgttcagcgt cagcgtgcct tcattcaggt tgacggcttt ctgggtgagt
     3421 gtggtgttgc tgacagtgag tgtgccactt cctgattttt ccacgctgcc gttaccggtg
     3481 agagagcctc cctgcaggag tgcatcgcct tcacggatgg tcagggtatc gttgttcacc
     3541 gtcttcccgg aaagggtaag tatggcgccg ttattcagcg tggtggtgcc cgccagtgtg
     3601 ccgcccctgg cggtgaacag tccgccattt accgtggtat ccgtggccgt atcaccggcg
     3661 ttcagcgtga atgaactgcc tttttccagc atcagggcat ccgcctgacc gcctccgata
     3721 ctgaatgcct ttccgtcgct ccgggtacca ctgacagcgg caccggaatc ggccagcagt
     3781 acaccgccat ttcccatgga tacggtggtg gcggtgccac cgttgcggac atccagcgtt
     3841 ccgccatcat ccacgcgggt attagtggct gtgtgtccgg tcagcacatc caggcgtccg
     3901 ccattttcca gtacgacatt atcagcttta ccctccacaa cagagaatgc tcccaggcgg
     3961 tttatgccgg taacggttgc agccgtactg gtaaccagtg cgccgccctg cttcagggtg
     4021 acattcgtgg ctgtaccacc ggcgtccacc tgcagtctgc ccttctgatt aacggtggta
     4081 ttcccggcca cacccccgtt tttgacaatc tgccagccgt cactgttcac aacagtgtca
     4141 gacgctgtac cgccgttgtg cacatactgg tatcccccat tcagcgtggt atccagtgcg
     4201 tgaccatgta cagtctggtc gccgccggca taaaccacag tggtatttgc cgttccttca
     4261 gctctcacaa tctggcgacc gtttttattg atggttgtgc gtacggcatc cccgcgaaca
     4321 aactgcccgg tatcaccgtt ttctgcatcc ggtccccctt ccgccccggt attaacaacg
     4381 gtatccgttg ccactgtacc gggcttgacg acctgccagc ccttatcatt aatgacggtt
     4441 cctgtggcta tcgccccctc atgcatccac tgttcgccac cattcagcgt ggtgttcact
     4501 gcccgtccct gaaggctctg tccgcctccg gcactgataa ccgtgtctga gacacttcca
     4561 ccggggttca ctctctgaag accaccactg gtgacagtcg ttttgttggc tgttccgcca
     4621 tcctgtaccc attgcccgcc ggtattggcc tcgttatccg gcccatactc cagcccggta
     4681 ctgatggtca ttccgttggt cgtaccgaag acaatctggt tgtcatgatt tgccagtgtt
     4741 ccgccgttca cggtttctcc cgggtgcaca acgatgtaag cagccagcac cgggagtgac
     4801 gtgactgcgg caagagacag tgcaaccgcc acaccgccac gtttaccccg tgcgcgggcc
     4861 agttcggagg caaccacgaa agcgcccgta atgtgattcc ataccagcct gtagcaggta
     4921 ttcagatgtc gtttcatcag cttttcctta gattgagggt gaataaaaaa gccggtaccc
     4981 acaaacgtgg ataccggcag gtaaagggtt acagtgccgg aggtccggct gtgggagttt
     5041 ctgaattgtg gttctgttgt tccctgaatg tctttctgta cgtatctgtg catgttactg
     5101 acaaaggtta ttattagctt atcgatcgat ataaacgatc gataaaatag atatcgatcg
     5161 ttttattcta ttattgagct attgcacgga caagagagac agtaacgaaa acagtgcttt
     5221 tgtgagcaga ctcagagtca gggagggcat cagtcacgac aggttcagaa gaaaatccag
     5281 ttccataccg cacgggcaac agagaccacc gtgtcacgaa cagcgcgcag ggccgtacgg
     5341 acaacagagg caacacagac gctctccgct gtgtcaaata tccggtccac cgcaccggta
     5401 aactgttcac gggcctgaga gcggacagat tccgtgcgca gctcgtcctg cagtcgggtc
     5461 atcaggggac tggcggcatg gtcgggaagc gctgtcatga gcgcactgac cagcgtatcc
     5521 agttcccagc cggtgcgggc cgatacggcc acaaccggat gtacgggccg gaacagacgg
     5581 aataccgcct ccgttttttc gcgaatattc tgtgcctgtg caggagaagg ctgaataccg
     5641 gccatatccc attcatggca gggctccgtt ttgtcggcct gcgtcaccac aaacagtacc
     5701 cgctgatgtc cccggtgcag gatgtgtcgc cagaaatact catccacaga cagggcacgg
     5761 tcatcggctt taatcagcca cagtaccagg tcaagttcag gcagaatgtc acggtacagg
     5821 gcctcatact ctgcatccct gtcccggctc tcgcccaccc cgggcaggtc agtgataacc
     5881 atgctgtgac cgtggccact cagacggaag cgccgtactt cccgggtgcc ggcgtgaaca
     5941 tcactgaccg gggtgacctc cccctgaaac agtgcattac agagtgagga tttaccggcc
     6001 ccgcttttac ccataatgcc aatcacgggt tcgtgacggg tgagtttgtg cagatgttcc
     6061 aggatgtgac gggaaagcga gtaaggcagg gaggagagcg gtttttcaat tgcctcaatg
     6121 gcatcagacg gattcataca tgtattcccg gtgaaaaaag acaaaacccc gcgcaccgga
     6181 aaggtggcgg gggattcacg gagataatat ctgtctgaaa ttgtttttaa tcgatgggat
     6241 tgttgcgtat taacatgaag gagatcagga cttatccgtt actgggtgat ctccttccat
     6301 gaaaatacgc cgttattgtt aaacagcagg ctgatttctc cgtaaagggg aaaatattta
     6361 tcggctgcat gattatcagg tgtgtctgtg tttactgctg tgagtatttt tcagtgtatt
     6421 gtgggggaga tgtcagtggg agatattaaa tttacattta taattatctt acttcttctg
     6481 tgtgtttttt ataagagtgc acacgtaatg atatctgttg ataccatgtg gttaagtgtt
     6541 tcccttcctg gtcatgcgta atgattaaat gcttttgtga caccattctt ccggtctgaa
     6601 tatttatccc gttctttttt atttgtgcag tgatactgtc ggaagagaat tcagtaatgt
     6661 gaggtgtcct ggtcaggata atatctgctg agatattatt agttgagtgt gtttttactg
     6721 agcaaaagct ggcttttaat tcctgtactg aaacactgtc gctcgtccgg ggttaactga
     6781 aactggatga tgatgtaaaa tgaaggctgt ttttgtgttt gtcgaagaag tgttatttta
     6841 atccattctc atcattggct ggcgattata cagaagtatt tgaagaaaaa atggtgcatt
     6901 ttatctgcgt cagcagggag gaagtatttt attgatgtat cagtattaaa tggtttttgt
     6961 tatttataaa tatgtcatcc tgtccataga atattgtact cagcattcag tgcaggaact
     7021 gtagtcttct ttattttatt cttcctcagg agggggggga ttcctgaagg cgttattccc
     7081 ggtgttctgt ttatgattca tcccggcagg aaaccggtat ctgcaatacc cgggatgatg
     7141 ctttttgttc gtacgtggtt gtgtaaaatt tgaatagtgg atgtgctctg aagggaagga
     7201 ggggacacac agattcattt tgtctttctt cagcagcaaa acacactgtg gttattattt
     7261 aaccattaaa tcaacaaaaa tttttacctc atattgattt gactgctatt tttgctatta
     7321 atagccagaa ttgcagtgct tttatatctg tgtatagtga ggttactatg tcattcggtc
     7381 agccttgtga tgagtttccc ctgagttcgc tgcctccact cattcgtgat gccgttattg
     7441 aagctcagca aattacgcag gctccccttg ggcttgtggc agcgtctgcc ctgggcgctg
     7501 tttcacttgt ctgccagaat ctgattgatg tctgcagact gaatacactg cgggggccgg
     7561 tctctctttt tttcctgacg cttgccgagt ccggagaacg aaaaaccgca gtggacaaat
     7621 tattaatgaa gcccctgtac cagcaggaga tgcagttata ttccagatac aaaagtgaac
     7681 tggctgtctg gaaaaacaag gaagagcttt taaaggctca gaaaaaagca ttgttgtcaa
     7741 aactgaataa agaactccgc aaaggcgcgg atgagtcaga aaccctcaga caactggaag
     7801 tacttcagaa aaacagtgcg gaagaacctg tgaggtataa attcattttt aatgatgcca
     7861 cgactgcggc aattaagaat cagctttgtg gtaaatggcg ttcagtgggt attatgtcag
     7921 atgaagccgg tattatattt gatggatata ccctgtctga gttgcctttt attaacaaaa
     7981 tgtgggatgg ctctgtattg tctgtggaca gaaagaatga acccgaacag atgattgaaa
     8041 atgccagaat gacgttatcc ctcatggtgc aacccggtct ttttgaccga tatatggagc
     8101 gaaaaggttc tgttgccagg gacagtggtt ttcttgcccg ttgtcttatc agtaaacctg
     8161 caaccacgca ggggaaacgc tttattaacg gagctgtgat tccgggagga tcattaacgg
     8221 catttcatga acgattaatg gaactggcca gaggcagtat tgaaaaatcc agtgaggatg
     8281 aacggtattg tctgcatttt tcgcctgaag cgcaaaaaat atttattgag cactataatg
     8341 ttctggagca ggatttatcc ccttccggac ccttaagccc gtttcgggga catgtttcaa
     8401 aaaaaacaga aaacattgca agaattgcag cgttatttca gtatttcagc tatggtgaag
     8461 ggaaaatctc tgccgatatc atgacgtctg ctgtcgtaat aagttcgtgg tacacagatg
     8521 aatataaaaa attatttgcg ttacctgatg aatctgaact gcagcagaag gacgccgagg
     8581 agctgttcga ctggctcatt gaggagtgcc gcggagaatg cccaccccgt gtcaggaaaa
     8641 attatattct ccagtgtggc ccgggtcggt tcagaaacag gaaaaaactg aacgcattgc
     8701 tgaacattct tgaatctcag ttcaggcttt ctgtcgtgcc ggaaggaaaa accatgtatg
     8761 ttctgcttcc ccagatagcc agtttaaagt tgtctgatgt ctctggtatt tttacctcgg
     8821 gttatcacta caataagctg agagcgaagt aaccggtgag cagtttttct ccattatgct
     8881 tcagacttag atgattgatt attatgggga tttcatcacc gatattcaga tgtatgaata
     8941 tcggtgatga actaaagctc aggagctgcg tggtggtgat gaatcaacag gcatataact
     9001 gatacccgcc ggtcagaagt tccagcctgc gactttcctg tctgcgtgcc cgcagtccgg
     9061 cgttcaggtg cagcgtgtga cagaacgcac ggagagcgaa acggcaggca gacaggccgt
     9121 tcagtcagcc ctggcaccct gcccggatac cagcttccct gaaacaactg gcatcaggtg
     9181 cgcttatcgt caaccgttgt gcggtatgcc aggagcacgt actgatccat cagatccgtc
     9241 agtgctgcgc tctgcttctg acggttgatg atccattcct ccagctcttt ttctgatttc
     9301 cgtttcagat tctccgtcca gtcgtcaagg cagtttccgg tcattaaatc aagccagcaa
     9361 agccagacca gtttgaggcg caactccgtg ccaccaaact gacggaagag gctgatcagt
     9421 ttatttgcca gcgaacgctg cctcagacgt tcacattcag acaggagaat gccttccagc
     9481 atcggatgat gaaccagcag atgttcactg gtgcataaat tcactatcag atcatcatcc
     9541 aggggcggcg tggtccgttt gtccggacca taaatcagcc ggtgcagact ctggggtacg
     9601 atggcaatct ccggtttttc accattgaat gacacccacc cgggcgtcag attatggaag
     9661 tagtagatat tcggatgaca cacgccccag gatacggcgt gccgggtgaa gccggtcagc
     9721 catttctggt agctttcgtg gagcagctcg taacgggtct gatactgtgg tttcattttc
     9781 atattcctca cgggggataa caggcagcag aacgccgccg gcaggccgga gactgcgtct
     9841 gttgcagatg aacggttaag aattacggac gggactgtgc aatacgcgcc tgcagccagg
     9901 cttccacttc gcttttcagc catcgggagc tgcggcccat tttgatgggc gcaggaaagt
     9961 caccatcctt gatgagtttg taaaaccact tatcggtcag gccggtcagt tgagtgataa
    10021 atgccatgtc gaccatctgg tcatccatca gtgaaactgg ggtgagcatg agaatgttcc
    10081 tccggatgtt gttaaaccag ccggtgtggt catgaaaaca acgcaccacc ttcaccggca
    10141 taacaaccgg ggagaaacgt aaaaaataat cgggaatgtg accgccagga gataaacggg
    10201 gaggttacgg tggctgagcc gtaccggtat gccgggtggt tgttacggaa cgatgcttcc
    10261 gcacgcgcgc gcgataaaga agggatgatt ttcgtgagga agaggggaaa actggcgttc
    10321 tgataattgc agagttattt acggggcggt tacgatgggg cagaccgtgt ccggtgggca
    10381 ggcgacaggt atcaccgatg ggataagact gtct
//
LOCUS       DAPHCV010000139        10367 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.140, whole genome shotgun sequence.
ACCESSION   DAPHCV010000139 DAPHCV010000000
VERSION     DAPHCV010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.140"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            191..1099
                     /locus_tag="RH033_004019"
     CDS             191..1099
                     /locus_tag="RH033_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HDV2993133.1"
                     /translation="MKAPVSITSFRPQKSIAIVIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIESLKERLIVRQLPTQLENVFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIEDRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1145..3136)
                     /gene="tkt"
                     /locus_tag="RH033_004020"
     CDS             complement(1145..3136)
                     /gene="tkt"
                     /locus_tag="RH033_004020"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDV2993134.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRGIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3414..4172
                     /gene="loiP"
                     /locus_tag="RH033_004021"
                     /gene_synonym="yggG"
     CDS             3414..4172
                     /gene="loiP"
                     /locus_tag="RH033_004021"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HDV2993135.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVLGHEMGHVALGHVKKGMQVALGTNAIRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFTQRQESEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(4263..5183)
                     /gene="speB"
                     /locus_tag="RH033_004022"
     CDS             complement(4263..5183)
                     /gene="speB"
                     /locus_tag="RH033_004022"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HDV2993136.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5319..6050)
                     /locus_tag="RH033_004023"
     CDS             complement(5319..6050)
                     /locus_tag="RH033_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993137.1"
                     /translation="MKKGKVRSALIALAVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTTVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(6196..8172)
                     /gene="speA"
                     /locus_tag="RH033_004024"
     CDS             complement(6196..8172)
                     /gene="speA"
                     /locus_tag="RH033_004024"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HDV2993138.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(8181..8312)
                     /gene="yqgB"
                     /locus_tag="RH033_004025"
     CDS             complement(8181..8312)
                     /gene="yqgB"
                     /locus_tag="RH033_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HDV2993139.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            8448..8663
                     /locus_tag="RH033_004026"
     CDS             8448..8663
                     /locus_tag="RH033_004026"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993140.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLFSTRLEVATRKKRRVLFI"
     gene            8967..10121
                     /gene="metK"
                     /locus_tag="RH033_004027"
     CDS             8967..10121
                     /gene="metK"
                     /locus_tag="RH033_004027"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDV2993141.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         2566 a         2773 c         2612 g         2416 t
ORIGIN      
        1 aatcaacatt ttgattgttg ttgtatttgt gatacatatc gccatttatt tattgaatgt
       61 ccgaaaacat acttttttaa tgcaatgttt tttcgctata cccctttata gattctgaat
      121 tctcacctgt aggctgtttt ctcctcacct catgtgacat attcccacac tttactcaag
      181 gaagattgtc atgaaagcac ctgtttcaat aacctcattt cgcccacaaa aatctattgc
      241 tatcgtcatt ggcgtgcttg ccgtggttgt gctgccattt ctctcgtatt acaccgttaa
      301 tgaaggtgaa cgcggcattt tgctgcgcta cggaaaaatc gtaaaagttg ccgagccggg
      361 tctgggtttt aagatccctt ttatggaaag cgtggagaaa atttccacgc gtaatcaggc
      421 tgtggtttat cagggattac aggcctacag ccgcgaccag caaccggcac aaatgacagt
      481 atcagtcagc ttccacatta aaccttcaga agctggcgca gtttatacaa cctacaacac
      541 cattgaatct ttaaaagagc gtctgatcgt ccgccagctg ccaactcaac tggagaacgt
      601 tttcggccaa tataccgcca tcagcgcggt acaggatcgt acaaaactgg ttcaggatct
      661 gcaaaatgca atgcgtaaag ccgttgttgg cccggtggtg attgatggtg tccagattga
      721 aaatattgat ttttctgacg cttatgaaaa atcgattgaa gatcgaatga aagcagaagt
      781 cgccattgcc acacgtaagc aaaatctgga aacagagaaa atccaggcgc aaatagcagt
      841 aactcaggcc caggctgaag cagacagtaa actggcggca gccaaagcag aagctgagac
      901 tattcgcgta agaggcgcgg cagaggctga aactattcgt ttaaaaagtg cggctgaagc
      961 agaagcgatc cgcctgcgcg gtgaagctct gcgggataat cccggacttg ttgccctcac
     1021 cactgctgag cgttgggacg gtaagctacc cgataccatg atccccggca gtacagttcc
     1081 gtttatctct actaagtaaa aaaagggccg ctttcgcggc ccttctcaca aagcatctta
     1141 ccgattacag cagcgctttc gctttcgcaa caacgttgtc aacggtgaag ccgaactctt
     1201 caaacagcag ctctgccgga gcagattcac cgaaggtggt catgccgacg atagcaccgt
     1261 tcaggccaac atacttgtac cagtagtcag caatacccgc ttccaccgca acgcgtgcag
     1321 taaccgcttt cggcagtaca gactcacggt aagccgcatc ctgcttgtcg aacgcgtcgg
     1381 tagacggcat ggaaaccacg cgcgctttca cgccttcagc agtcagtttt tcgtaggcag
     1441 caaccgccag ttcaacttca gaaccggtcg cgatgaagat cagttccggc tgaccggcac
     1501 agtctttcag cacataacca ccgcgcgcga tgtttgccag ctgctcttca gtacgttcct
     1561 gctgcgccag gttctgacgg gagaggataa gcgcagtcgg gccgtcctga cgctcaacgc
     1621 cgtatttcca cgcgaccgcg gattcaacct ggtcacacgg acgccatgtg gacatgttcg
     1681 gggtcacgcg cagtgaagcg acctgctcaa ccggctggtg agtcgggcca tcttcgccca
     1741 gaccgatgga gtcgtgggtg taaaccatca cctgacgctg tttcatcagc gcagccatac
     1801 gtacggcgtt acgtgcgtat tccacgaaca tcaggaaggt ggaggtgtac ggcaggaagc
     1861 caccgtgcag ggagataccg ttagcaatcg cggtcatacc gaactcgcga acaccgtagt
     1921 ggatgtagtt acccgcagca tcttcgttga ttgctttaga accagaccac agggtcaggt
     1981 tagacggtgc caggtcagca gagccgccga ggaattccgg caacagcggg ccgaacgctt
     2041 caatcgcatt ctgagacgct ttacggctgg cgattttcgc cgggttagcc tgcagtttag
     2101 cgatgaactc tttcgctttg gcgtcgaagt cagacggcat ttcgcctttc atacggcggg
     2161 taaattcagc ggcttcctgc ggataagctt tcgcgtaagc agcgaatttc tcgttccatg
     2221 cggattcttt cgcctggcct gcttctttcg catcccactg agcatagatt tcagacggga
     2281 tttcgaacgg tgcatatttc cagcccagct gttcgcgggt cagggcaatt tcagcgtcgc
     2341 ccagcggcgc accgtgggag tcgtgcgtac cggctttgtt cggggaaccg aaaccgatga
     2401 tggttttgca catcagcagg gacggtttgt cagtcactgc gcgcgcttct tctactgcgc
     2461 gtttgatgga tgccgcgtca tgaccgtcga tgccgcgaat aacgtgccag ccgtaagctt
     2521 cgaaacgcat tgcggtgtcg tcggtgaacc agccttcaac gtgaccatcg atagagatac
     2581 cgttgtcatc gtagaacgca atcagtttac ccagcttcag cgtacccgcc agagaacaaa
     2641 cttcgtggga gatgccttcc atcatgcagc cgtcgcccat gaaggcgtag gtgtagtggt
     2701 cgacgatgtc gtggcccgga cggttaaact gcgccgccag cgttttttct gcaatcgcca
     2761 taccgactgc gttggcaata ccctgaccca gcggacctgt agtggtttcc acgccagcgg
     2821 tgtaacccac ttccgggtgg cccggagttt tagagtgcag ctgacggaag tttttcagtt
     2881 cttccatcgg cagatcgtaa ccggtgaggt gcagcaggct gtagatcagc atggagccgt
     2941 ggccgttgga cagcacgaag cggtcacggt cagcccagga cggattctgc gggttgtgtt
     3001 tcaggaaatc acgccacagg acttcggcaa tgtcagccat acccataggg gcacccgggt
     3061 gaccggattt ggctttctgt actgcgtcca tgctcagcgc acgaatagca ttggcaagct
     3121 ctttacgtga ggacattttg actccagatc ggatgatgaa gggcacgccc ttaacgactt
     3181 gacgacagcg cgttttgggc tacgccggaa aatttgccaa caatttaccg caagccgcgc
     3241 gtcatgtaca tggaacatcc ttttgccgct tcagaaatct ctggatcatg ctcgcatgtt
     3301 gcgcaatctg ctcgcccgtc cgctgcgctt ttccttatac tgagactgag cgtcgattca
     3361 cctgcaaacg gcgcattttt agaataatcc tgaccttgtg cggaagagaa aacatgaaaa
     3421 ttcgcgcctt attggtagca atgagcgtgg caacggtact gaccggttgc cagaatatgg
     3481 actccaacgg actgctctca tcaggagcgg aagcttttca ggcttacagt ttgagtgatg
     3541 cgcaggtgaa aaccctgagc gatcaggcat gtcaggagat ggacagcaag gcgacgattg
     3601 cgccagccaa tagcgaatac gctaaacgtc tgacaactat tgccaatgcg ctaggcaaca
     3661 atatcaacgg tcagccggtg aattacaaag tgtatatggc gaaggatgtg aacgcctttg
     3721 caatggctaa cggctgtatc cgcgtctata gcgggctgat ggatatgatg acggataacg
     3781 aagtcgaagc ggtgctcggt cacgaaatgg ggcacgtggc attaggccat gtgaagaaag
     3841 gaatgcaggt agcacttggt acaaacgcaa ttcgcgttgc ggcggcctct gcaggcggaa
     3901 ttgtcggcag tttgtcacaa tcacaactgg gagacctggg cgaaaagcta gtcaattcgc
     3961 aatttaccca acgtcaggaa tcagaggccg atgattactc ctacgatttg ctgcgccaac
     4021 gcggtatcag cccggcaggt cttgccacca gctttgaaaa actggcgaaa ctggaagaag
     4081 gccgccaaag ctcaatgttt gacgaccatc ctgcatccgc cgaacgcgcc cagcatattc
     4141 gcgatcgtat gagcgcggat ggagttaagt aaagccaggt agtgccgaac gtaggtcgga
     4201 taaggcgttc acgccgcatc cgacattaat ggcacatcac ccgtgcgcct cttatctggc
     4261 gcttactcgc cctttttcgc cgcctgaata tacagcatct ccagcgccag cgtcgccgct
     4321 gccagagcgg tgatttccga ctgatcgtat gccggagcca cctccactac gtccatccca
     4381 acgatgttga gatctttcag gccgcgtacc agtttaatgg cgcgatcgga ggtcaggccg
     4441 ccaattaccg gcgtaccggt gcctggtgca aatgcagggt ccaggcaatc gatatcaaag
     4501 gtcaggtaaa ccggcatatc acccacaatc tgtttcacct gggcgataac gtcatccacg
     4561 ctgcgatcgt tcacctggca ggcgtccagc acggtaaagc cgttgtcttt atcaaactcg
     4621 gtacgaatac caatctgcac ggaatgattc gggtcgatca gaccttcttt cggcgcggta
     4681 tagaacatgg tgccgtggtc aaattcacaa ccgttcgcat aggtatcggt gtgggcgtca
     4741 aagtgtacca gcgccatttt gccgaaatgc ttcgcatgag cacgcagcag cggcagcgta
     4801 acaaagtggt caccaccgaa ggagagcata cgcttaccgg cagccagcag cttctcggcg
     4861 tgtgcctgca gcttctcgct catctcacgg gcatcgccga aggcatatac cagatcaccg
     4921 cagtccacca cgttcaggcg ctcacgcatg tcgaaattcc acgggaagcg gttgtgttcc
     4981 caggccagat tcgtcgaaac ctgacggatc gctgccggac cgtggcgccc ccccgcacgg
     5041 ccagaagtgg ccatatcgaa cggcacgcca gtaatcaccc agtcagcatc gctgtcatac
     5101 ggctggaagt tcatcggcag gcgtaaaaaa ccaaaggcgt tggaaaccag tgagttatcg
     5161 tattgatgac ctaaggtgct catggatgga ctcctattac aaagtcgata taaaaaaacc
     5221 cttccgcgtc gttaggcccg acgaggaagg gttggatttg cgaaacacaa ttttgtgcta
     5281 attatcgcct taaatgggcg aggcatcaag cgaggttgct atttccgaca cggtggcggc
     5341 gcatagccat tgttatctgc cggaaaggct tgtccagagc acggcggcag cgcgttttgc
     5401 gccgggcctt gtaattcaaa ggcggcaaag ccgattacac cggtattacg cacatcgcct
     5461 tgcgccgaat tggctgcgta ggaatcagca acgttggcga actgaaaagc cgcttcggta
     5521 tgtttatctt tgcggaatcc tttaatcgct aacgaatctc cggcattcac aatatagccg
     5581 ttgttattca gcgaaccttg cttgccgttc agcacatcca ggccatcaac cgtagcaacg
     5641 atctcgtagt tggtgttacg actgtaattg cggacatata attgataatt cattcctact
     5701 ctggcatgga caatatagcg tggttcatta tttgacgaat cataaacccg cgtaataggt
     5761 agagaattaa aattaccatc gcgaatagca tattcaagtt cgttaatacg aatgctataa
     5821 acgcggtcgt agcccgaagg aacagtcgtc gaataactaa taacgatcat ctctgccgga
     5881 tcgcgccaag ctcgctcagc agatacgccc tgaaccgttg agtgcacatc cccaccccag
     5941 gcggtgccga cattgcctga tgcactgcta ttatactgcg cattactgga acaaccggcc
     6001 agcagcacgg ctaatgctat caacgcgcta cgtactttcc cttttttcat aaccgttccc
     6061 tctgcattta attaatgcag cgattgtagg gagattattt aataagaaaa gcagctaaat
     6121 tatttttaag aatatataaa aagaaagcgg gccgatttac gtaaataaat cgacccgcca
     6181 gcggaataat cttaattatt catcttcaag ataggtataa ccgtacaaac ctgcctcgaa
     6241 ctcttcaagg aactgttgtt gcagttcagc atcaagatcg gttttcttca cttgatcacg
     6301 gaactgggtt aacaacgttt tcggatcgag ctgtacatat tgcagcatat ccgccacggt
     6361 atcgccttcg tcagacagtt ctacttctac gctgccgtca gggaagacga acacgtcaac
     6421 cgcttcggta tcaccgaaca ggttgtgcat gttgccgagg atctcctgat atgcgccgac
     6481 catgaagaaa ccgagcatcg gcggattctc tggatcgtac tccggcattg gcattgtcgt
     6541 ggcaatacca tcaccatcga tgtagtgatc aatagcaccg tcagaatcac aggtaatatc
     6601 cagcagcaca gcgcggcgct ccggcacttg atccaaccct tccagcggca gaaccgggaa
     6661 caactggtcg atcccccatg cgtccggcat cgactggaac agcgagaagt tgacgtacat
     6721 tttgtcagcc atacgttcct gcagctcgtc gataatcgga cgatgcgcac ggttttgcgg
     6781 atccagttgc ttctgcactt catggcacat gctcagatag agttgttctg cccatgcacg
     6841 ttcttgcagg ctaaaggtgc cggaagagta gccgatatga atatcgtgca gatccatctg
     6901 gctgtcgtgt aaccattcac gcagagaacg acgagttccc ggttcgtgca tctcctgcca
     6961 ggtttcccac atgctttgca gggcgcgcgg tgcgtcttct gcaggcgcgg tcggcaccgt
     7021 gtattcgtta cgttccacgc cgatgatatt cgacaccagc acggtgtgat gcgcagtcac
     7081 cgcacgaccc gactcggtga ttaccgtcgg atgtggcaga ccattttctt cacacgcatc
     7141 accaatcgcc cagatgatgt tgttggcgta ttcattgagg ccgtagttca ccgaacagtc
     7201 ggactgcgaa cgggtgcctt catagtccac gcccagaccg ccgccaacgt cgaagcactg
     7261 aatattgacg cccagcttgt gcagttccac atagaaacgc gcggattcac gaacgcctgt
     7321 cgcgatatcg cgaatattag ccatctgcga accgaggtgg aagtgcagca gttgcaggct
     7381 gtcgagacgc ccggcttcac gcagggtttc aaccagttgc agtacctgag tcgccgccag
     7441 gccgaacttc gatttttccc cgccggagga ctgccattta cccgagccct gcgaagccag
     7501 acgtgcacgc acgcccagac gaggaacgac attcagacgt tctgcttcat ccagcacaat
     7561 ggcaatttct gacatcttct caatgaccag atagaccttg tggcccatct tctcgccaat
     7621 taatgccagg cggatatatt cgcggtcctt ataaccgttg cagacgatga cgctacgggt
     7681 catgccagca tgtgccagca ccgccatcaa ctccgctttg gaaccggctt ccaggcccag
     7741 cggttcgccc gaatgaatca gggactcaat cacgcggcgg tgctggttaa ctttgatcgg
     7801 ataaacgagg aagtaatcgc cgttatagcc gtaggattcc cgcgcacgtt tgaacgcggc
     7861 gttaatagaa cgcaaacggt gctgcaggat ctgtgggaaa cagaacagtg caggcagacg
     7921 ctggccctgt gcttcacgag ttttcactaa ctgcgcgaga tcgacgcgag cttccgggac
     7981 gtccgggtcc gggcacacgc taatgtggcc cagctcgtta acgtcatagt agttattgcc
     8041 ccaccaggca atattgtaag tacgcagcat cttgctggct tcctgggagc tcattgcaac
     8101 ctcctgcatg gagcgtagta caccgtgttc gcccgctgac gaaggcaaac ccatagacat
     8161 gtcgtcagac atagcgaacc tcaaattatt ttattaagtg taaaacagtt aacgactatc
     8221 gcagcctgga actgcgataa caacccataa gcacatggat tttccagcag tgaatgctga
     8281 cgctccaact gcgcaaccgg tttctttttc ataacattat taagcacata accgaacgta
     8341 agtgtgacag ttcggcgaaa ccacgagaaa actcttgttt ttacaagagc gcccttgttc
     8401 agtcctcagt agctgtaacc agctcttgaa tcctgagaag cgccgagatg ggtataacat
     8461 cggcaggtat gcaaagcaga gatgcagagt gcggggaacg aatcttcacc agaacggtga
     8521 gacaggttaa gcagcagaca acggttcatt atttcgtatc acctccacgg ccgcctgtta
     8581 agacgaaccc acaagccaaa actctgtttt caacccggct ggaagtggca acacgaaaga
     8641 aacgtcgtgt gctttttatt taagccgcgc gccgcgtttt ataccccaca atggcagaaa
     8701 attgcaaaag ataaatacgc agaatgccag cattgtcagg aaaaatttcc agccacgttt
     8761 ttaacagaat gagacacgat tcaaaaaaaa gtggaaatcg ggtgaagaat tgacctaaaa
     8821 tagccatcca gatgttaatc catccatacc gattaacact cagactgcca gtgtttttaa
     8881 cctgcagagt cgtggtagga tccgctacca cagaaaatcc acacaacagt ttgagctaac
     8941 caaattctct ttaggtgata ttaaatatgg caaaacacct ttttacgtcc gagtccgtct
     9001 ctgaagggca tcctgacaaa attgctgacc aaatctctga tgccgtttta gacgcgatcc
     9061 tcgaacagga tccgaaagca cgcgttgctt gcgaaaccta cgtaaaaacc ggcatggttt
     9121 tagttggcgg cgaaatcacc accagcgcct gggtagacat cgaagagatc acccgtaaca
     9181 ccgttcgcga aattggctat gtgcattccg acatgggctt tgacgctaac tcctgtgcgg
     9241 ttctgagcgc tatcggcaaa cagtctcctg acatcaacca gggcgttgac cgtgccgatc
     9301 cgctggaaca gggcgcgggt gaccagggtc tgatgtttgg ctacgcaacc aatgaaaccg
     9361 acgtgctgat gccagcgcct atcacctatg cacaccgtct ggtacagcgt caggctgaag
     9421 tgcgtaaaaa cggcactctg ccgtggctgc gcccggacgc gaaaagccag gtgactttcc
     9481 agtatgacga cggcaaaatc gttggcattg atgctgtcgt gctttccact cagcactctg
     9541 aagagatcga ccagaaatcg ctgcaagaag cggtaatgga agagatcatc aagccgattc
     9601 tgcccgctga atggctgact tctgccacca aattcttcat caacccgacc ggtcgttttg
     9661 ttatcggtgg cccgatgggt gactgcggtc tgactggtcg taaaattatc gttgatacct
     9721 acggcggcat ggcacgtcac ggtggtggtg cattctctgg taaagatcca tcaaaagtgg
     9781 accgttccgc agcgtacgca gcacgttatg tcgcgaaaaa catcgttgct gctggcctgg
     9841 ccgatcgttg tgaaattcag gtttcctacg caattggcgt ggctgaaccg acttccatca
     9901 tggtggaaac tttcggtacc gagaaagtgc cttctgaaca actgactctg ctggtacgtg
     9961 agttctttga cctgcgtcca tacggtctga ttcagatgct ggatctgctg cacccgatct
    10021 acaaagaaac cgcagcatac ggtcactttg gtcgcgaaca tttcccgtgg gaaaaaaccg
    10081 acaaagcgca gctgctgcgc gatgctgccg gtctgaagta atctttcttc acctgctttc
    10141 aaaggccagc ctcgcgctgg cctttttctt ttacctcacc gcggtggata gacattcacg
    10201 ccgcatccgg caaaaaaacc gcccgcacaa taacatcatt cttcctcaac acgttttacc
    10261 gcaaattatc atcattactg aaaccgatta caccaaccac accagccaaa tatttgtaat
    10321 attttcatat tattattcag ttttcacagt tgttacattt cttttca
//
LOCUS       DAPHCV010000140        10294 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.141, whole genome shotgun sequence.
ACCESSION   DAPHCV010000140 DAPHCV010000000
VERSION     DAPHCV010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.141"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            114..1508
                     /gene="galP"
                     /locus_tag="RH033_004028"
     CDS             114..1508
                     /gene="galP"
                     /locus_tag="RH033_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HDV2993142.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKHELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            1585..2082
                     /locus_tag="RH033_004029"
     CDS             1585..2082
                     /locus_tag="RH033_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HDV2993143.1"
                     /translation="MKTSRLPIALQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            2177..2884
                     /gene="endA"
                     /locus_tag="RH033_004030"
     CDS             2177..2884
                     /gene="endA"
                     /locus_tag="RH033_004030"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HDV2993144.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     VAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWDKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            2964..3695
                     /gene="rsmE"
                     /locus_tag="RH033_004031"
     CDS             2964..3695
                     /gene="rsmE"
                     /locus_tag="RH033_004031"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDV2993145.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQLDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            3708..4658
                     /gene="gshB"
                     /locus_tag="RH033_004032"
     CDS             3708..4658
                     /gene="gshB"
                     /locus_tag="RH033_004032"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HDV2993146.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMADLY
                     LINGEARARTRTLSVEQNYDKWYEFTGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEDKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWEIARRVGPTLKAKGLIFVGLDIIG
                     DRLTEINVTSPTCVREIEAEFPVSITGMLMDAIEARLQKS"
     gene            4767..5330
                     /locus_tag="RH033_004033"
     CDS             4767..5330
                     /locus_tag="RH033_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HDV2993147.1"
                     /translation="MNLQHHFLIAMPALQDPLFRRSVVYICEHNPDGAMGLIINKPLE
                     NLQVEGILEKLKITPEPRDPAIRLDKPVMLGGPLAEDRGFILHTPPSRFASSIRISDN
                     TVVTTSRDVLETIGTPKQPADVLVALGYSSWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            5330..5746
                     /gene="ruvX"
                     /locus_tag="RH033_004034"
     CDS             5330..5746
                     /gene="ruvX"
                     /locus_tag="RH033_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDV2993148.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(5920..6900)
                     /locus_tag="RH033_004035"
     CDS             complement(5920..6900)
                     /locus_tag="RH033_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HDV2993149.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPAVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVKEGRL"
     gene            6918..7622
                     /gene="yggS"
                     /locus_tag="RH033_004036"
     CDS             6918..7622
                     /gene="yggS"
                     /locus_tag="RH033_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HDV2993150.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            7640..8206
                     /gene="yggT"
                     /locus_tag="RH033_004037"
     CDS             7640..8206
                     /gene="yggT"
                     /locus_tag="RH033_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HDV2993151.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            8203..8493
                     /locus_tag="RH033_004038"
     CDS             8203..8493
                     /locus_tag="RH033_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HDV2993152.1"
                     /translation="MSAVTINDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALLN"
     gene            8501..9094
                     /locus_tag="RH033_004039"
     CDS             8501..9094
                     /locus_tag="RH033_004039"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HDV2993153.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            9087..10223
                     /gene="hemW"
                     /locus_tag="RH033_004040"
     CDS             9087..10223
                     /gene="hemW"
                     /locus_tag="RH033_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HDV2993154.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAANLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFSQYTGLS
                     EEVIRSQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         2416 a         2721 c         2698 g         2459 t
ORIGIN      
        1 ccaagataaa tgttagtgta agcgattaca ctgatgtgat ttgcttcaca tctttttacg
       61 tcgtactcac ctatcttaat tcacaataaa aaataaccat attggagggc atcatgcctg
      121 acgctaaaaa acaggggcgg tcaaacaagg caatgacgtt tttcgtctgc ttccttgctg
      181 ctctggcggg attactcttt ggcctggata tcggtgtaat tgctggcgca ctgccgttta
      241 ttgcagatga attccagatt acttcgcaca cgcaagaatg ggtcgtaagc tccatgatgt
      301 tcggtgcggc agtcggtgcg gtgggcagcg gctggctctc ctttaaactc gggcgcaaaa
      361 agagcctgat gatcggcgca atcctgtttg ttgccggttc actgttctct gcggctgcgc
      421 caaacgttga agtactgatt ctttcgcgcg ttctgctggg gctggcggtg ggtgtggcct
      481 cttataccgc accgctgtac ctctctgaaa ttgcgccgga aaaaattcgc ggcagtatga
      541 tctcgatgta tcagttgatg atcactatcg ggatcctcgg cgcttatctt tctgataccg
      601 ccttcagcta caccggtgca tggcgctgga tgctgggtgt gattatcatc ccggcaattt
      661 tgctgctgat tggtgtcttc ttcctgccag acagcccacg ttggtttgcc gccaaacgcc
      721 gttttgttga tgccgaacgc gtgctgctac gcctgcgtga caccagcgcg gaagcgaaac
      781 acgaactgga tgaaatccgt gaaagtttgc aggttaaaca gagtggctgg gcgctgttta
      841 aagagaacag caacttccgc cgcgcggtgt tccttggcgt actgttgcag gtaatgcagc
      901 aattcaccgg gatgaacgtc atcatgtatt acgcgccgaa aatcttcgaa ctggcgggtt
      961 ataccaacac taccgagcaa atgtggggga cagtgattgt cggcctgacc aacgtacttg
     1021 ccacctttat cgcaatcggc cttgttgacc gctggggacg taaaccaacg ctaacgctgg
     1081 gcttcctggt gatggctgct ggcatgggcg tactcggtac aatgatgcat atcggtatcc
     1141 actctccgtc ggcgcagtat ttcgccatcg ccatgctgct gatgtttatt gttggctttg
     1201 ccatgagtgc cggtccgctg atttgggtac tgtgctccga aattcagccg ctgaaaggcc
     1261 gcgattttgg catcacctgc tccaccgcca ccaactggat tgccaacatg atcgttggcg
     1321 caacgttcct gaccatgctc aacacgctgg gtaacgccaa caccttctgg gtgtatgcgg
     1381 ctctgaacgt actgtttatc ctgctgacat tgtggctggt gccggaaacc aaacatgttt
     1441 cgctggaaca tattgaacgt aatctgatga aaggtcgtaa actgcgcgaa atcggcgctc
     1501 acgattaatc tcctcaggct tcctcccgtc gtggaggaag ccacctcttg cagtcatctt
     1561 ttcttcgctc tatcctctgc cgctatgaaa acatcccgtc tccctatcgc cctccaacag
     1621 gccgttatgc gtcgcctgcg ggaaaaactc gcccaggcga acctgaagct agggcgtaac
     1681 tacccggagc caaaactctc ttacacccag cgcggaacct ctgccggaac agcctggctg
     1741 gaaagctatg aaattcgcct caatcccgtt ttgctgttgg aaaacagtga agcctttatt
     1801 gaagaagtgg tgccgcacga actggcacat ttgctggtat ggaaacattt cggccgcgta
     1861 gcaccacatg gcaaagagtg gaagtggatg atggaaagcg tgctgggcgt tcccgcccgt
     1921 cgtacgcatc agtttgaact gcaatccgtg cgccgcaaca ccttccccta ccgctgtaag
     1981 tgccaggaac atcagcttac cgtacgccgc cataatcgcg tagttcgtgg cgaagccgtc
     2041 tatcgctgtg ttcactgcgg cgaacagctg gttgcgaaat aaccacctga actatcagga
     2101 actttcctga tctggctgat tgcataccaa aacaactttc gctacgttgc tggctcgttt
     2161 taacacggag taagtgatgt accgttattt gtctattgct gcggtggtac tgagcgcagc
     2221 attttccggc ccggcgttgg ccgaaggtat caatagtttt tctcaggcga aagccgcagc
     2281 ggtaaaagtc cacgctgacg cgcccggtac gttttattgc ggatgtaaaa ttaactggca
     2341 gggcaaaaaa ggcgttgttg atctgcaatc gtgcggctat caggtgcgaa aaaatgaaaa
     2401 ccgtgccagc cgcgtagagt gggaacacgt cgttcctgcc tggcaattcg gtcaccagcg
     2461 ccagtgctgg caggacggtg gacgtaaaaa ttgcgccaaa gatccggtct atcgcaagat
     2521 ggaaagcgat atgcataacc tgcagccgtc agtcggtgag gtgaatggcg atcgcggcaa
     2581 ctttatgtac agccagtgga atggcggtga aggccagtac ggtcaatgcg ccatgaaggt
     2641 cgatttcaaa gaaaaagttg ccgagccacc agcgcgtgca cgcggtgcca ttgcgcgcac
     2701 ctacttctat atgcgtgacc aatacaacct gacactctct cgtcagcaaa cgcagctgtt
     2761 caacgcatgg gacaagatgt atccagttac cgactgggag tgcgagcgtg atgaacgcat
     2821 cgcgaaggtg cagggcaatc ataacccgta tgtgcaacgc gcttgccagg cgcgaaagag
     2881 ctaacctaca ctagcgggat tctttttgtt aacccctacc ccacgcgtac aaccgcgtgg
     2941 ggagacgacg cggattttta actatgcgta tcccccgcat ttatcatcct gaaccactaa
     3001 ccagccattc tcacatcgcg ctttgcgaag atgccgccaa ccatatcggg cgcgtactgc
     3061 gcatggggcc gggacaggcg ttgcaattgt ttgacggcag caaccaggtc tttgacgccg
     3121 aaattaccag cgccagtaaa aaaagcgtgg aagtgaaagt gctggaaggc caactcgacg
     3181 atcgtgaatc tccgctgcat attcacctcg gtcaggtgat gtcgcgtggt gaaaaaatgg
     3241 aatttactat ccagaaatcg atcgaactcg gtgtaagcct cattacgcca cttttttctg
     3301 agcgctgcgg cgttaaactg gatagtgaac gtctgaacaa gaagctccag cagtggcaga
     3361 agattgcaat tgctgcctgt gagcagtgtg gccgtaaccg ggtgccggaa atccgtccag
     3421 cgatggatct ggaagcctgg tgtgcagagc aggatgaagg actgaaactg aatcttcacc
     3481 cgcgcgccag taacagcatc aatacgttgc cgttaccggt cgaacgcgtc cgcctgctga
     3541 ttggcccgga aggcggttta tcggcagatg aaattgccat gactgcccgc tatcaattta
     3601 ctgatatcct gttgggacct cgcgttttgc gtactgagac aactgcgctc accgccatta
     3661 ccgcgctaca agtgcgtttt ggcgatttgg gctaacggag aagaataatg atcaagctcg
     3721 gcatcgtgat ggaccccatc gcaaacatca acatcaagaa agattccagt ttcgctatgt
     3781 tgctggaagc acagcgtcgt ggttacgaac ttcactatat ggagatggcc gatctctatt
     3841 tgatcaatgg tgaggctcgc gctcgtaccc gtactttgag cgtcgaacag aactacgata
     3901 aatggtacga gttcacgggt gagcaggatc tgccgctggc cgatctcgat gtgatcctga
     3961 tgcgtaaaga cccgccgttt gataccgagt ttatctacgc gacctatatt ctggaacgtg
     4021 ccgaagataa agggacgctg atcgttaaca agccgcagag cctgcgcgat tgtaacgaaa
     4081 aactgttcac tgcctggttc tctgacttaa caccagaaac gctggtcaca cgcaataaag
     4141 cgcagctaaa agctttctgg gaaaaacaca gcgacatcat tcttaagccg ctggacggta
     4201 tgggcggcgc gtcgattttc cgcgtgaaag aaggtgatcc aaacctcggc gtgattgccg
     4261 aaaccctgac tgagcatggc actcgctact gcatggcgca aaattacctg ccagccatta
     4321 aagatggcga caaacgcgtg ctggtagtgg atggcgagcc ggtgccgtat tgcctggcgc
     4381 gtattccgca ggggggcgaa acccgtggta acctggctgc cggtggtcgt ggggaaccac
     4441 gtccactgac ggaaagcgac tgggaaattg cccgccgtgt tggccctacg ctaaaagcca
     4501 aagggctaat ctttgtgggg ctggatatca ttggtgatcg tctgaccgaa attaatgtta
     4561 ccagcccaac ctgcgttcgt gagatcgagg cggaatttcc ggtatcgatc accggaatgt
     4621 taatggatgc catcgaagca cgtttgcaga aatcttaaat aacctccgcg ggacggagcc
     4681 cataaagact aagagtgaca gcgcccatct ttccccgcat actgggcgct gttgcttttt
     4741 tgaaccagga aacagaacct ctgacaatga atttacagca tcactttctt attgccatgc
     4801 ctgctctcca ggatccgctt ttccgccgct cagttgttta catttgcgag cataatcccg
     4861 atggcgcaat ggggctgatt attaataagc cgctggaaaa tctgcaggtc gaaggaattc
     4921 tggaaaagtt aaaaattacg ccagaaccgc gcgatcctgc cattcgtctg gataagcccg
     4981 ttatgcttgg tggcccgctg gcagaagatc gtgggtttat tttgcatacc ccgccgtcac
     5041 gctttgcctc cagcatccgt atttctgata acactgtggt gaccacttcc cgcgatgtcc
     5101 tggaaacgat aggaactcct aagcagccag ccgatgttct ggtcgcgctg ggttactcct
     5161 cctgggagaa aggccaactg gaacaagaaa ttctcgataa cgcatggcta acggccccgg
     5221 cagatctgaa tattctgttc aaaacgccga ttgccgaccg ctggcgcgag gcggcaaaac
     5281 tgattggtgt ggatattctc accatgcctg gtgtggcagg gcacgcctga tgagtggaac
     5341 cttactcgcc ttcgacttcg gcaccaaaag cattggcgta gcggtcggcc aacgcattac
     5401 cggcaccgct cgccctttgc ctgcaattaa agcacaggac ggtacgccgg actggaacct
     5461 catcgaacgt ttactgaaag agtggcagcc ggacgaaatc atcgtcggtt tgccgctgaa
     5521 tatggacggc accgagcaac cattgactgc cagagcgcgt aaatttgcca accgtattca
     5581 tggccgtttc ggtgttgaag taaagctcca tgacgaacgt cttagcaccg tagaagcacg
     5641 ttccggtctg tttgaacagg gcggctatcg ggcgctcaac aaaggcaaag ttgactctgc
     5701 ctctgcggtt atcattctcg aaagttattt cgagcaggga tattaaggcg atttactagc
     5761 gcacagattt ctgacaatgt gctcgttgtt tcatgttgga tgcggcgtaa acgccttatc
     5821 caacctacag tgagtgcgaa ttcaaagtta cgcttccctt gtaggcccga taagcttgcg
     5881 catcgggcat atttacaatt cgtatcagac acagcaacgt cacaagcgcc cttctttcac
     5941 ccgctgctga taactctgct gaaacgttat catccccacc tgctgcccgg tttgaataac
     6001 atgcggtaac tggtgggttt tcccttcacg aatcaaattc cccaccgcgg gtgtgttaat
     6061 cagcaattca aacaacgcca cgcgtccttc ctgtttatcc acttccagct tttgtgacaa
     6121 caccgcccgt aaactacctg ccagttgatt acgcacggga tctttttcct gcgccggaaa
     6181 tgaatctacc agtcgctcaa ctgcctgcgc cgcaccacgc gtatgtaatg ttgccagcac
     6241 caaatgcccg gtttctgccg ccgtcagcgc cagacggatt gtctcgctgt cacgcagctc
     6301 tccgagcaaa atcacatcag gatcttcccg caatgcagcc cgcaatcccg atgcgaacgt
     6361 catacaatgc aaaccgattt cccgctgctg gatcaaacat cgctggcttg catagagata
     6421 ttcgacagga tcttccagcg tcagaatatg cgcatcggca tgttgattga gatagccaac
     6481 catcgccgcc agcgtggtag atttgccact ccccgtcgcc cccgtcacca gaatcaggcc
     6541 attctcgctc ttgagtaatt ccggcaatac cgctggtgcg ccaagctgtt cgagctgcgg
     6601 gcagtgcgaa ggtaacagcc gtaacgccag cgaaatgcct tgccgttgcg cgaacgcact
     6661 gccacgcaat cgctggtttt ccgccagcga cacggcaaaa tccagctgac cattctccag
     6721 caatattgcc cgctgatcgt catccagcca ctcccgcagt agctcttcga cgtccggcgc
     6781 atcaaacggc gcagcttcca ttcgcccgcg aatacgccat cgtgcgggcc aggcgctgca
     6841 caggtgtaga tccgagacgt tatgctttac actaagggcc acaatttctt ccatattcat
     6901 actaagatcc tcggaaaatg aacgatattg cgcataacct ggcacaggtc cgggacaaaa
     6961 tctcagcggc tgcaacgcgt tgcggccgtt ctccagaaga aattacgctg cttgcagtca
     7021 gtaaaacaaa acctgcgagc gccatcgcag aagccattga tgccgggcag cgtcaatttg
     7081 gtgaaaacta cgttcaggaa ggggtagata aaattcgcca ctttcaggaa ctgggcgtaa
     7141 caggattaga atggcatttt attggcccgt tgcagtctaa taaaagccgc ctggtggcag
     7201 agcatttcga ctggtgtcat accatcgacc gtttgcgcat tgctacccgc ctcaacgatc
     7261 agcgcccggc agaacttccc cctcttaacg tactgattca aattaatatt agtgatgaaa
     7321 acagtaagtc cgggattcaa ctggctgaac tggacgagct ggcagctgcg gtcgctgaac
     7381 taccgcgttt acgtctgcgc gggctgatgg caatccctgc acctgagtca gaatatgtaa
     7441 ggcagtttga agttgcacgc caaatggctg tagcatttgc cggactgaaa acacgctacc
     7501 cgcatatcga cacgctctct ctgggaatgt cggacgatat ggaagccgcc attgcggcag
     7561 gtagcacgat ggttcgtatc ggcactgcaa tttttggtgc gcgtgattac tctaaaaaat
     7621 aaggaattaa aggaacgcca tgaatacgtt gactttcctg ctttcaacgg tcattgagct
     7681 gtataccatg gtgctgttat tacgcatctg gatgcagtgg gctcattgtg atttttacaa
     7741 ccccttctca cagtttgtag tgaaggtaac gcagccaatt atcgggccac tgcgccgcgt
     7801 tattccggca atggggccca ttgacagcgc ctcactgctg gttgcgtaca ttctcagttt
     7861 catcaaagcc atcgtgctgt ttaaagtggt gacctttctg ccaatcattt ggattgccgg
     7921 tttactgatt ctgctgaaaa ccatcggcct gctgattttc tgggtcctgc tggtgatggc
     7981 gattatgagc tgggtaagcc aggggcgtag cccgattgaa tacgtgctga ttcagctggc
     8041 cgatccgctg ctgcgcccga ttcgccgcct gctaccggca atgggtggga ttgatttctc
     8101 gccgatgatc ctcgttctgc tgttgtatgt catcaatatg ggtgtcgcag aagtattaca
     8161 ggcaaccgga aacatgctgc tgccggggct gtggatggcg ttatgagtgc cgtaacaatt
     8221 aatgatgacg gtctggtttt acggctctat attcagccga aagccagccg tgattctatt
     8281 gtcggtttac atggcgacga ggtaaaagtc gccataaccg cgccgccggt tgacggccag
     8341 gccaacagcc atctggtgaa gtttctcggt aagcaattcc gggttgccaa aagccaggtg
     8401 gtgattgaaa aaggcgaact tggccgccac aaacaaatta aaatcattaa tccgcaacaa
     8461 atcccgccag aaatcgcggc gttacttaat taggtatcct atgcaaaaag ttgtcctcgc
     8521 aaccggcaat gccggtaaag tgcgtgagct ggcgtcgctg cttagcgact tcggtcttga
     8581 tatcgtggcc caaacagacc tcggcgtaga ttccgctgaa gaaaccggcc tgacctttat
     8641 cgaaaacgcg attctgaaag cgcgccatgc ggcaaaagtg accggtttac cggcaattgc
     8701 cgacgactcc ggtctggcgg tagatgtact tggcggtgcg ccgggaattt actccgcgcg
     8761 ttattccggt gaagacgcga ccgatcaaaa gaatctgcaa aaattgctgg aaacactgaa
     8821 agacgtaccg gacgaccaac gtcaggcacg tttccactgc gtgctggtat atctgcgtca
     8881 cgcggaagat cccactccgc tggtgtgcca cggcagctgg ccgggtgtga ttactcgtga
     8941 accagcgggt actggtggct ttggttatga tccaattttc ttcgttcctt ccgaagggaa
     9001 aaccgctgcc gaactgaccc gcgaagaaaa gagcgccatt tcccaccgtg gtcaggcgtt
     9061 gaaactgctg ctggacgctt tacgtaatgg ttaaattacc gccgctgagt ctctacattc
     9121 acatcccgtg gtgcgtacag aaatgcccgt actgcgattt caactctcac gcgttgaaag
     9181 gagaagtgcc gcacgacgat tatgttcagc atctgcttaa cgatctggac aacgatgtgg
     9241 cttacgctca gggccgtgaa gtaaagacaa tctttattgg cggtggtacg ccgagcctgc
     9301 tttccggccc ggcgatgcaa acgctgctgg acggcgtgcg tgcgcgtttg ccgctggcag
     9361 cggatgcaga aattactatg gaagcgaacc ctggtacggt agaagccgat cgctttgttg
     9421 attatcagcg tgctggtgtg aaccgcatct ccattggcgt gcagagtttt agcgaagaaa
     9481 agctgaaacg acttgggcgc attcatggcc cgcaagaagc gaaacgagcg gcaaatctgg
     9541 cgagcggttt agggttacgt agctttaacc tcgatttgat gcatgggcta ccggatcaat
     9601 cactggaaga ggcgcttggc gatctgcgcc aggccattga actgaatccg ccgcatcttt
     9661 cctggtatca actgaccatc gaacctaata cgctgtttgg ctcgcgccct cctgtactgc
     9721 cggacgatga cgcgctgtgg gatattttcg aacaggggca tcagttatta accgcagcgg
     9781 gctatcagca gtatgaaaca tccgcttacg ccaaaccagg ttatcagtgc cagcacaatc
     9841 tcaactactg gcgatttggc gactacatcg gtattggctg cggcgcgcac ggcaaagtga
     9901 ccttcccgga tgggcgcatt ctgcgtacca ccaaaacgcg tcatccgcgt ggttttatgc
     9961 aaggaaggta tctggaaagc cagcgtgatg tcgaagccgc agataagccg tttgagttct
    10021 ttatgaatcg cttccgtctg ctggaggccg cgccgcgcgt ggagtttagc cagtatactg
    10081 gcctttctga agaagttatt cgctctcagt tagacgaggc catcgctcag ggttatctca
    10141 cagaatgtgc ggattactgg cagataaccg agcatggtaa attattctta aactcacttc
    10201 ttgaattatt cctcgctgag taatcattaa tgcgcctgcg ggcgcattaa tcacttcttt
    10261 tacccccccc ccgttaatta acttcggcaa aaat
//
LOCUS       DAPHCV010000141        10270 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.142, whole genome shotgun sequence.
ACCESSION   DAPHCV010000141 DAPHCV010000000
VERSION     DAPHCV010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.142"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1843)
                     /gene="selB"
                     /locus_tag="RH033_004041"
     CDS             complement(<1..1843)
                     /gene="selB"
                     /locus_tag="RH033_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDV2993155.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEMLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTSLWLADNDRIVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDIHSHHGWLHLPDHKAGFSAEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(1840..3231)
                     /gene="selA"
                     /locus_tag="RH033_004042"
     CDS             complement(1840..3231)
                     /gene="selA"
                     /locus_tag="RH033_004042"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDV2993156.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVTKAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(3329..3937)
                     /locus_tag="RH033_004043"
     CDS             complement(3329..3937)
                     /locus_tag="RH033_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HDV2993157.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(4008..5144)
                     /locus_tag="RH033_004044"
     CDS             complement(4008..5144)
                     /locus_tag="RH033_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDV2993158.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNA
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(5147..5509)
                     /locus_tag="RH033_004045"
     CDS             complement(5147..5509)
                     /locus_tag="RH033_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HDV2993159.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVAPQGDVPDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            6046..7959
                     /gene="mtlA"
                     /locus_tag="RH033_004046"
     CDS             6046..7959
                     /gene="mtlA"
                     /locus_tag="RH033_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDV2993160.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            8188..9336
                     /gene="mtlD"
                     /locus_tag="RH033_004047"
     CDS             8188..9336
                     /gene="mtlD"
                     /locus_tag="RH033_004047"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDV2993161.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVDAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGTLPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            9336..9923
                     /gene="mtlR"
                     /locus_tag="RH033_004048"
     CDS             9336..9923
                     /gene="mtlR"
                     /locus_tag="RH033_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HDV2993162.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(9934..10143)
                     /locus_tag="RH033_004049"
     CDS             complement(9934..10143)
                     /locus_tag="RH033_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993163.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRQLYRPKQVEEK"
BASE COUNT         2426 a         2805 c         2658 g         2381 t
ORIGIN      
        1 atttttccgg aaataataat gcgtcgcgca ataaatgatc atttccacga cgacgcgtaa
       61 agccaatgcg gtcaaaatat tccagaatct gaattgccag ctttcggcct acgcctaagc
      121 gatcgcggaa atccgccgcg caggttgaac cacattcctg atcgagatcg cggatcatat
      181 tggcaaactc gacaatccga tcgttacggt aataacgatc tttgacgatc gcagtaatta
      241 tcccctgttg cgccgcctgg cgtagcgtca ggcgcattgc ctgctcgtcg gttcccgtct
      301 cttttgccag gtcacgcacc caccatggct cgtcaccaaa cagcggctct gctttttgcc
      361 agatggcctg ctgctctgca ctgaagcccg ctttatgatc cggcagatgc agccagccgt
      421 gatggctgtg gatgtcgccg ctttcgcgca tcttttcaat cagcaacagt accagcgctt
      481 catcttccat tggcaacgcc atacgtcgca gacgttcgcg cccaggacca ggttcatcgc
      541 gatgttgctc atgataagtc gctaatgtat cgagaatttt ccgctgccag cgggcggcaa
      601 ctggcgcatt caacaagctg taaccagcct gaatatagcc aggctgttgc agcagttcgc
      661 gcatcccttc gccgttgagc tgccgtgccc aggcgaaatc cgcaaggtta accgcgccgc
      721 gttccagatg aacagataac gcatcggcat cgctctgcgc ccgtgcaaga gacgccagcc
      781 attgcagata ttccggctta cgcttaccgc gacgcggcgg gttaagcatc accacacgcg
      841 cccccgccag cgtgttgcgg gccgagatat cgcgcagcac tatgcgatcg ttatccgcca
      901 gccacagcga cgtgtcgaac accagctcgg caaggttatc ttccagtagc gaaacacgtc
      961 ccgtaacgtg gctggcggcg tggtgaatat gcagcggctg ccactgggtc agcggtgtat
     1021 gggtttgaag ctcgacaatc acccgtgtga acggctccgg cggcacatcg gcaagcagcc
     1081 agtcgccacg gttaatctgc tctttttccg catcacccgc gatgttaagc gcgatacgct
     1141 gcccggcatg ggcagtttct gttggctgat tttgcgcatg cagcgcacgc acgcgcatcg
     1201 gtttatttac accagtcagc cagagtgaat cgcctacttt cacttccccg cttaacgccg
     1261 taccggtgac gaccagcccg gcacctttta cagtaaatgc gcggtcgatc gcgaggcgga
     1321 aactatgttg gctggcgtgt tcacgttctg gtaactgaag cagatgctcg cgcagggcat
     1381 caattccccg accttcggtt gctgcggtga taaacagttt tgcctcagca aaaccgtatt
     1441 cccgcagcat ctcctttacc tggcgttcaa cctcatcaac acgcgcttcg tccacgcgat
     1501 cggctttggt cagcgccact gtcagcatcg ggttaccggt cagctgcaaa atcgccagat
     1561 gctcacgggt ctgtgccatc acaccgtcat cgcacgccac caccaacagc gcgtgatcga
     1621 taccaccaac gcccgccagc atgttggaaa gaaacttttc atgaccgggt acgtcgataa
     1681 aaccaggcac gcgaccatca ggctgcggcc agtaggcata gccgagatcg atggtcatgc
     1741 cgcgcttttt ttcttccggc agacggtcgg catttacgcc cgtaatcgcc tgcaacaagg
     1801 ttgttttgcc gtggtcaacg tgtccggcag tcgcaataat catttcaaca acatctccaa
     1861 aaaccgttgc tcatcttcaa ggcagcgtaa atccagccac aatcgtccgt cataaatacg
     1921 accaatcacc ggcactggca attcacgcca gcgggcggct aatgactcaa ggtggctacc
     1981 gcgtccatca tggggtgtaa acgttaatgc cgcgctcggc aggcgatcaa ccggcagtga
     2041 accactgcca atctgcgaaa gacatggcat aacctggact gcaaactccg cgccgtaatg
     2101 tgcggcaagg ggagcctgta aacgttgtgc ctggatttga atgacctctg cgctgcgggt
     2161 aagcaggcgc agggtcggta atttttcact cagagcttca gggtgtaaat aaagacgcaa
     2221 cgtggcttcc agcgccgcga gggtcatttt atccgcgcgt aatgcacgct tcagcgggtg
     2281 gctttgcagg cgggcgatca tctctttttt accaacgata atccctgcct gcggcccgcc
     2341 taacaacttg tcaccggaga aactcaccag actgacgccc gccgcaatca actcctgcgg
     2401 cattggctct ttcggcaaac cgtactggct aagatcaacc agcgagccac tgcctaaatc
     2461 agtcactacg ggaacatcca gctctttgcc gagcgccacc agttccgctt catctatcgc
     2521 tttggtgaat ccctgaatgc tgtagttact ggtatgtact ttcatcaaca gcgcggtatt
     2581 ttcattcacc gcctgacgat aatcattagc gtgcgtgcgg ttggtggtcc ctacttcgtg
     2641 tagggtgcat cctgcctgac gcataacatc gggaatacga aacgcgccgc caatctccac
     2701 cagttcgccg cgagaaacca ccacctcttt cccgctggca gtggccgcca acatcaataa
     2761 caccgccgcc gcattgttat taacgataca ggcatcttcc gcccccgtaa tacggcacag
     2821 cagctgcgcc agcgcccgat cgcgatgtcc gcgtccggcg tcgtccagat cgtactcgag
     2881 ggtcactggc gaacgcatag ccttcgtaac ggcttccacc gcggcttccg cctgtaaagc
     2941 tcgcccaagg ttggtatgca gcacggttcc cgtcaggttg attaccggac gcagcgcgct
     3001 ttgcgcctct ttcgtcaacc gggcatcgac ttcttgcgcc cagttttcac accacgcggg
     3061 cagcgtctgg ctgtctcgaa tcacttctcg cgcttcgtca agcatctgac gcagcaattc
     3121 caccacgcgg gtgtgaccat aagtatcacg caaagaaagg aaggagctat cgcgcaataa
     3181 gcgatcaata gccggaagtt gactatagag ggaacgcgtt tcggttgtca taggaaacct
     3241 ggctgatcaa ggccctctca cacggagaag ggcgtttaac ataaccacgg attgtaacgt
     3301 gagatgggtc aggaggacat atcgcgcatc aagcctttgg cggttcggtg cgagcaaaac
     3361 tttcgcggct aaacaggttt tcgaccagtt tgactaagtg tgggcggtcg acacaccagc
     3421 ctggcgcaac gcggcggaaa ttgagatatc cgacggcaca ggcaatggcg atagtcgcca
     3481 gattgaccgt atcggttttg agcgtgccat cgaccagata tccttccagc acatccagac
     3541 tgcggttgat tttctcccgc tggcgtaaca attcatcttc agactgctgc gccgccggac
     3601 gcgcctgttc acgcaccgat accagcccgg catccataat gccatccgcc agcgcctcaa
     3661 ttttgcgcac ccgcaacgac tccagcggat cacgcggcaa catcgccgga gcaacattca
     3721 ttaattcaat atattcagcg atgatcggcg aatcaaacca gcattcgccc tcttcggtca
     3781 ccagcaccgg cacttttcct aacgggttaa attgcgccac gccgttatcc gcgttatagg
     3841 gcagttcatt aataaattcg aacgttatgc ccttttctaa caacagaata gaaagtttgc
     3901 gtacaaacgg gctggtgtag ctaccgacga gtttcatgcc gagtcctttg tgcgaggaaa
     3961 aatatcagta tggctcaggc tcgcgcaaaa cacaggtgat acaagtctca atgatccaaa
     4021 taaagataat gcatccaact ggtcattctt agcagcactt tccgcatcac cgaaacatgg
     4081 ctgaaatggt cggagtaaac tgccacctgg gcgtagatgc cgtcgggtaa ggcatcgata
     4141 tcagcgttag ggtccagttc aatggttccc agcacaccgt ccgtgccggg cacgaccgtt
     4201 aatgattgca ataccccctg cgcctgataa gaaccgcctg gcacgacagg taaaatactg
     4261 gtcagtttgc cgtgaaacac ctgcccaggt agcgcgttaa acaccacttc cgcatcatcg
     4321 ccgggtttca gacgtaacag cgagttttgc cgaaattggg cgacaatttg ccgtttttgc
     4381 tcggggatga agaccatcac cggacgcagc ggcaaggcag cggcgtatgt acctgggcgg
     4441 atcagtacct gagtgacgta gccattgctc ggtgcgcgaa tgacggtctg ctcaaggtta
     4501 tattttgctt cagtaagttg cgctcttaag ctcacaatct gcgattgctc gccgttaacc
     4561 atactgtcga gctggctctg gatctgcgcc tgctccgcca ccgagccttt caccagcgca
     4621 tcctgcgcga ggaaattttg ccgcgcatcg tcgatgtcac gttccgagaa cggattcacc
     4681 gccgcctggc tgcctttcag ataacgttga taatttttaa acagacggtc gcgctccgct
     4741 gaaacctggg tggtgttggc ctgcgcttca gtgagctgcg cgcgcagcgt ctttatatta
     4801 tgcgtcgccg tcatcaggtc agcctgaagc ctgtcaactc gcgcctggta acgaaccggg
     4861 tcgagcttaa aaagcacctc gcccttctga ataagctgat tatttttgtc ggtgacttca
     4921 gtaacaattc ccgtcacctg tggcgtgatg gggatcgcta tcactgcctt ttgcgcggta
     4981 aaagtgtaag ggtggttgta gttcatcaac aaaatcaaac cactcaccag aaacacgcct
     5041 cccagcgccg ccgtcgccag cgtccactga ttcaccggaa tgcggaagat tttaaagacc
     5101 gcccacgcca gcgccacgta agttaaaaca atcaatagat ccataattag atctccggaa
     5161 acgtggaata gtcagtcttt ttctcggcgg cgagttggtg ctccagccgg gaaatccggt
     5221 cagaaagtgc ggcgatttcc gggtctggca cgtcgccttg cggcgcaaca tgcgactgca
     5281 tgccccagcc gcgctccggt tgatagagcg tcgcccagat ccacaaaaac ggccagataa
     5341 catgcagagt aaacaggctc acccagcccg ccgtatgaat agcgtcggca tggggatggt
     5401 tgcgcttttt ggcaatcaga taagggatgt catgtatcgc gatgattcca taaaaaatca
     5461 ccagaaagac gaagatcagc actcccaacg cgaaatagtt tagaaacata tctgcctcgg
     5521 attcacgttt atcagtgttg ttttttggct ggcagccaga agggagtcag gctgatgttt
     5581 tgacaataat ccgggttcgc gattctcgcc ataacaccaa agaataattt taagaggtga
     5641 tgagttgctt agttacataa cgatttcatg acgaaggcat aacatgctgt agatcacatc
     5701 aggtgaacgc cgtaagaaaa tatcttgtga ttcagatcac aaagattcaa caaaccatca
     5761 aaataaaaat gtgacataac tcacatttaa atgccatttt tagcgaaaat cgccgccttg
     5821 ttgctttttt acacaagcat tttgtgatga gaatcacgtc aattacctct ctacccccta
     5881 catttatgtg actgatatca cacaaaaggc cgccgactgg acagttaacc gattcagtgc
     5941 cagatttcgc agtatctaca aggtccggct acctctgccg ccacattaac aaaaaacctc
     6001 gggcttccag cctgcgcgac agcaaacata agaaggggtg tttttatgtc atccgatatt
     6061 aagatcaaag tgcaaagctt tggtcgtttc ctcagcaaca tggtgatgcc aaatatcggc
     6121 gcgtttatcg cgtggggtat catcaccgcg ttatttattc caacagggtg gttaccgaac
     6181 gaaacgctgg cgaagctggt cggcccgatg atcacttatc tcctgccgct gctgatcggt
     6241 tataccggtg gtaagctggt aggcggcgaa cgtggcggcg tagtcggtgc catcaccacc
     6301 atgggcgtta tcgtcggcgc agacatgccg atgttcctcg gttcgatgat tgcaggtcca
     6361 ctgggcggct ggtgcattaa gcacttcgac cgctgggtag acggtaagat caaatccggt
     6421 tttgagatgc tggtgaataa cttctccgca ggcatcatcg ggatgatcct cgctattctg
     6481 gcattcctcg gcattggccc gattgttgaa gccctgtcca aaatgctggc tgcgggcgtt
     6541 aacttcatgg ttgtccatga catgctgccg ctggcgtcta tctttgttga accggcgaaa
     6601 atcctgttcc tcaacaacgc cattaaccac ggtatcttct cgccgctggg tattcagcag
     6661 tcccatgaac tgggtaaatc catcttcttc ctgattgaag ctaacccagg tccgggtatg
     6721 ggcgtgctgc tggcgtacat gttctttggt cgtggtagcg ctaaacagtc tgcgggcggt
     6781 gcggcaatca tccacttcct ggggggtatc cacgaaatct acttcccgta tgtgctgatg
     6841 aatccgcgtc tgattctggc cgttatcctc ggcggtatga ctggcgtgtt cacgctgact
     6901 atcctgggcg gtgggctggt ttctccggca tctccgggtt ctatccttgc tgtactggcg
     6961 atgacgccaa aaggtgctta cttcgctaac atcgcgggtg tgtgtgcggc gatggcagtc
     7021 tccttcgttg tctctgctat tttgctgaaa accagcaaag tgaaagaaga agatgatatt
     7081 gaagcagcaa ctcgtcgtat gcaggacatg aaagctgagt ctaaaggtgc gtctccgctg
     7141 tctgcgggcg atgtgactaa cgacctgagc cacgtacgta aaatcatcgt tgcctgtgac
     7201 gccggtatgg gttccagtgc gatgggcgca ggtgtgctgc gtaagaaaat tcaggatgca
     7261 ggtctgtcgc agatttcggt cactaacagc gcgatcaaca acctgccgcc agatgtggac
     7321 ctggtcatca ctcaccgtga cctgaccgaa cgcgctatgc gccaggttcc gcaggcacag
     7381 catatttcgc tgaccaactt cctcgacagc ggcctgtaca ccagcctgac cgaacgtctg
     7441 gttgctgccc agcgccatac tgaaaacgaa gtgaaagtga aagacagtct gaaagacagc
     7501 tttgacgatt ccagtgctaa tctgttcaaa ctgggcgcgg agaacatctt cctcggtcgc
     7561 aaagcggcca ccaaagaaga agcgattcgt tttgctggcg agcagctggt gaaaggcggt
     7621 tacgttgagc cggaatatgt tcaggcgatg ctggatcgtg aaaaactgac cccgacttat
     7681 ctgggtgagt ctatcgctgt gccacacggt acggttgaag caaaagatcg cgtactgaaa
     7741 acgggcgtgg tgttctgcca gtacccggaa ggcgtgcgct tcggtgaaga agaagatgac
     7801 attgcccgtc tggtgattgg tattgctgcc cgtaacaacg agcacattca ggttatcacc
     7861 agcctgacca atgcactgga tgatgaatcc gtcatcgagc gtctggcaca taccaccagc
     7921 gtggatgaag tgctggaact gctggcaggt cgtaagtaat ccaatcccac ctctccacat
     7981 ggagaaggtg gggcttattg cctgatgcgc tacgcttatc aggcctacaa aacaaagcgc
     8041 agtttgttga atttgcacgt tcttgtaggc cggataaggc gtttacgccg catccggcgc
     8101 ggcccctctc cacatggaga gggtttgggt gagggaaaag cctcacccca gccctctcgg
     8161 gtaaaaacat tgatgaaggt taatactatg aaagcattac attttggcgc aggtaatatc
     8221 ggtcgtggct ttatcggtaa actgctggcg gacgcgggta tccaactgac gtttgccgat
     8281 gtaaatcagg tggtacttga tgccctgaat gcccgtcata gctatcaggt tcatgtggtt
     8341 ggtgaaaccg agcaggtaga taccgtttct ggcgtcgatg ccgtcagcag cattggcgat
     8401 gatgtcgttg atctgattgc tcaggtcgat ttagtcacta ccgccgttgg ccctgtcgtg
     8461 ctggaacgta ttgctccggc catcgccaaa gggctggtga aacgtaaaga acaaggtaat
     8521 gaatccccac tgaacatcat cgcctgtgaa aacatggtcc gcggcaccac gcagctgaaa
     8581 ggccatgtga tgaacgccct gccggaagat gccaaagcgt gggtagaaga acacgttggc
     8641 tttgttgatt ccgccgttga ccgcatcgta ccgccttcgg cttcggcaac taacgatccg
     8701 ctggaagtga cggtagaaac cttcagcgaa tggattgtcg ataaaacgca gttcaaaggc
     8761 acgctgccaa acatccctgg catggaatta accgacaatc tgatggcatt tgtcgaacgt
     8821 aaactcttca ccctgaatac aggtcatgct ataaccgcgt acctcggaaa actggcgggt
     8881 catcagacca ttcgcgacgc gattctcgac gagaaaatcc gcgcggtggt taaaggtgcg
     8941 atggaagaaa gcggcgcggt actgatcaag cgctacggct ttgacgcgga caaacatgcg
     9001 gcgtacatcc agaaaatcct cggtcgtttc gagaacccgt atctgaaaga tgatgtagag
     9061 cgcgtaggcc gtcagccgct gcgtaaactg agtgctggcg accgtctgat caagccactg
     9121 ctcggaacgc tggaatatgg tctgccacat aaaaacctga ttgaaggtat tgccgctgca
     9181 atgcacttcc gcagtgaaga tgatccgcag gctcaggaac tggcagcact gatcgctgac
     9241 aaaggtccgc aggcggcgct ggcacagatt tccggtcttg atgccaacag cgaggttgta
     9301 tccgaggcgg taaccgctta taaagcaatg caataatggt ggaccaggcg caggataccc
     9361 tgcgcccgaa taacagattg tcagatatgc aggcaacaat ggaacaaacc caggcctttg
     9421 aaaaccgtgt gcttgagcgt ctgaatgctg gcaaaaccgt gcgaagcttt ctgatcaccg
     9481 ccgtcgagct tttgaccgag gcggtaaatc ttctggtgct tcaggtattc cgcaaagacg
     9541 attacgcggt aaagtatgct gtagaaccgt tactcgacgg cgacggtccg ctgggcgatc
     9601 tttctgtgcg tttaaaactc atttacgggt tgggtgtcat taaccgccag gaatacgaag
     9661 atgcggagct actgatggca ttgcgtgaag agctaaatca cgacggcaac gagtacgcgt
     9721 ttaccgacga cgaaatcctt ggaccctttg gtgaactgca ttgcgtggcg gcgttaccac
     9781 cgccgccaca gtttgaacct gcagactcca gtttgtatgc aatgcaaatt cagcgctatc
     9841 aacaggctgt gcgatcaaca atggtccttt cactgacaga gctgatttcc aaaatcagct
     9901 taaaaaaagc ctttcaaaag taagtacttt cgcttacttc tcctctacct gtttcggacg
     9961 ataaagctga cgataatact ccagacgttg tagatataac ggcacgcttt catcaggtat
    10021 tccggacgga atcgcgttac ggggaggtag ttttttcaga tactcccgga acgcctggct
    10081 cgatgccatg aaatctacgg ctttatcgat aagaagcgga acgttttcac ctaatttgcc
    10141 catgatgata tctccgtatt accccgcgcc gggaatgcgc ggccgccaat tttagtttag
    10201 atcccggtat aaatgacatt aaggaaaacg tgctcaatcc tcaataatca cctgcttctg
    10261 ttgactatct
//
LOCUS       DAPHCV010000142        10224 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.143, whole genome shotgun sequence.
ACCESSION   DAPHCV010000142 DAPHCV010000000
VERSION     DAPHCV010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.143"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            114..656
                     /gene="ecpR"
                     /locus_tag="RH033_004050"
                     /gene_synonym="matA"
     CDS             114..656
                     /gene="ecpR"
                     /locus_tag="RH033_004050"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HDV2993164.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKLYKLVQ"
     gene            731..1318
                     /gene="ecpA"
                     /locus_tag="RH033_004051"
                     /gene_synonym="matB"
     CDS             731..1318
                     /gene="ecpA"
                     /locus_tag="RH033_004051"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HDV2993165.1"
                     /translation="MKKKVLAIALVTVFTGTGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLSNGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1375..2043
                     /gene="ecpB"
                     /locus_tag="RH033_004052"
                     /gene_synonym="yagY"
     CDS             1375..2043
                     /gene="ecpB"
                     /locus_tag="RH033_004052"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HDV2993166.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIQNS
                     TDSGRLINIRLERLSSPLDDGQVIAMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            2069..4594
                     /gene="ecpC"
                     /locus_tag="RH033_004053"
                     /gene_synonym="yagX"
     CDS             2069..4594
                     /gene="ecpC"
                     /locus_tag="RH033_004053"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HDV2993167.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMTLANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQIWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTFSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQSVYSGTFGSLGLRAGIQRYNNGDSSANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFDTGLENDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4584..6227
                     /gene="ecpD"
                     /locus_tag="RH033_004054"
     CDS             4584..6227
                     /gene="ecpD"
                     /locus_tag="RH033_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HDV2993168.1"
                     /translation="MRVNLLIAMIIVALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6196..6905
                     /gene="ecpE"
                     /locus_tag="RH033_004055"
                     /pseudo
     CDS             6196..6905
                     /gene="ecpE"
                     /locus_tag="RH033_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
     gene            7219..7548
                     /locus_tag="RH033_004056"
     CDS             7219..7548
                     /locus_tag="RH033_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDV2993169.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARFTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(7797..8411)
                     /locus_tag="RH033_004057"
     CDS             complement(7797..8411)
                     /locus_tag="RH033_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HDV2993170.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNSAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            complement(8626..8811)
                     /locus_tag="RH033_004058"
     CDS             complement(8626..8811)
                     /locus_tag="RH033_004058"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993171.1"
                     /translation="MASGLICHGGFEPHAWDVANLMDVAVLVVAYLLVWLLVNRENHY
                     ARGIPQDVQVLHKKAHH"
     gene            8860..9018
                     /locus_tag="RH033_004059"
                     /pseudo
     CDS             8860..9018
                     /locus_tag="RH033_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(9235..9928)
                     /locus_tag="RH033_004060"
                     /pseudo
     CDS             complement(9235..9928)
                     /locus_tag="RH033_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         2773 a         2551 c         2496 g         2404 t
ORIGIN      
        1 cttatttact tccgatttac taaagaaact gaatgtacct gtaaaaatta caggtttgga
       61 aagtagtgac atggcaaaat gattacagca gggactatga ggttaaaaac catatggaat
      121 gtcaaaaccg ttctgataaa tacatctggt ctccccatga cgcctacttc tataaaggac
      181 tatctgaact gattgtggat atcgacagat taatttatct atcgctggag aaaatcagaa
      241 aagatttcgt gtttatcaat ctcaatacgg attctttaac tgagtttata aaccgtgata
      301 atgagtggtt atccgcggta aaggggaaac aggtcgtatt gattgcggcc agaaagtcag
      361 aagccttagc aaattattgg tattacaaca gcaatattag gggcgtggta tacgctggac
      421 tgagtcgtga tattagaaaa gaactggcct atgtgattaa tggcaggttc ctgagaaaag
      481 atattaagaa agataaaatc actgaccggg aaatggaaat tatccgcatg acggctcagg
      541 gaatgctgcc taaatcgatt gccagaattg aaaattgtag tgtgaagaca gtgtataccc
      601 atcggcggaa tgcagaggcc aagctgtact caaaattata taagttggtt cagtaaactc
      661 caggcaagtt agttttaaaa aatgactcac cgggacttca tgtcctcaat tcaactcggg
      721 aagaaaagca atgaaaaaaa aggttctggc aatagctctg gtaacggtgt ttaccggtac
      781 aggtgtagcg caggctgctg acgtaacagc tcaggctgta gcgacctggt cagcaacagc
      841 caaaaaagac accaccagta agctggttgt gacgccactc ggtagcctgg cgttccagta
      901 tgccgaaggc attaaaggtt ttaactcaca gaaaggtcta tttgacgtgg ctatcgaggg
      961 tgactcaacg gctaccgcct ttaaactgac ctcacgtctt atcaccaaca ccttaaccca
     1021 gttggatacc tcaggatcca cactgaatgt gggcgtggat tataacggcg cggcagtcga
     1081 aaaaactggc gataccgtga tgatcgatac cgccaacggc gtactgggcg gcaaccttag
     1141 cccactgtct aacggttaca atgccagcaa tcgtaccacc gcacaggatg gtttcacttt
     1201 ctccatcatc agcggcacca ccaatggtac caccgcagta accgattaca gcactctacc
     1261 ggaaggcatc tggagcggcg acgttagcgt acagttcgac gctacctgga ccagttaatc
     1321 tctctgatgt accagcaggg gtacccccct gctttattcc tggacggaat tgttatgaaa
     1381 aagcaccttc tgcctctcgc tctgctgttt tccggaatat caccggccca ggcgctggat
     1441 gtcggcgata tatcatcgtt tatgaacagt gacagcagca cgctaagcaa aacgatccaa
     1501 aacagtaccg acagtggccg cctcatcaat atccgtctcg aacggctctc ttcaccgctt
     1561 gacgacgggc aggttatcgc aatggacaag ccggatgagt tgctactcac tcccgccagc
     1621 ttgctgctac ccgcccaagc cagcgaagtg atccgcttct tctataaggg accagcagat
     1681 gaaaaagagc gctactaccg cattgtctgg tttgatcagg ccctcagtga tgcgcagcgc
     1741 gataatgcca accgcagcgc tgtggccact gcttccgccc gcatcggcac cattctggtc
     1801 gtcgcccccc gccaggcaaa ctaccacttt cagtacgcca acggctccct gacaaataca
     1861 ggtaatgcga cgctgcggat cctcgcctac ggcccctgcc tgaaagccgc caatggtaag
     1921 gagtgtaaag agaattacta cctgatgccg ggcaagtcgc gtcgttttac ccgcgtggac
     1981 acggcggata acaaaggacg ggttgcactt tggcagggtg ataagttcat tcccgtgaaa
     2041 tagatagctg tgcagatgga taacgacaat gcctttacga cggttctccc caggactgaa
     2101 agcccagttt gccttcggca tggtcttttt gttcgttcag cccgatgcca gcgctgctga
     2161 cataagtgcg cagcaaatag gtggggtgat tattccgcag gccttcagtc aggcgcttca
     2221 ggacggcatg agcgtcccgc tctatattca tctcgccggt agccagggtc gccaggacga
     2281 tcagcgaatc ggcagcgctt ttatctggtt ggatgatgga cagttacgca tccgaaaaat
     2341 acagctggaa gagagtgaag ataacgccag tgtcagcgaa caaactcgac agcagctgat
     2401 gactctggcc aacgccccgt tcaatgaggc ccttaccatc cccctgactg acaacgcgca
     2461 gttggatctc agcttgcgcc aactgctgct gcagctggtg gtcaagcgcg aagcgctggg
     2521 caccgtacta cgctcacgta gcgaagacat cgggcagtcc agtgttaata ccctcagcag
     2581 taatctgagc tataacttcg gcgtctataa caaccagttg cgtaacggcg ggagcaacac
     2641 ctccagctat ctgtcgctga ataacgttac tgcgctgcgc gaacatcacg tggtgctcga
     2701 cggctcactg tacgggattg gtagcggtca acaggacagt gaattatata aagcgatgta
     2761 tgaacgcgat tttgctggtc accgatttgc cggtggaatg ctcgacacct ggaacttgca
     2821 gtccttaggg ccgatgaccg ccatttcagc agggaagatt tacggccttt cctggggaaa
     2881 ccaggccagc tccaccatct tcgacagcag ccaatcggcc acgccagtga tcgccttttt
     2941 accggcggcg ggcgaagtac atctcacccg tgatgggcga ctactaagcg ttcagaactt
     3001 caccatgggc aatcatgaag tggatacccg gggtctaccg tacggtattt acgatgtgga
     3061 agttgaggta atcgttaacg gtcgcgtgat cagcaaacgc acccagcggg tcaataagct
     3121 gtttagccgg gggcgtggcg tcggtgcacc gctggcgtgg cagatatggg gcggtagctt
     3181 tcatatggat cgctggtcgg aaaacgggaa aaagacgcga ccagccaaag agagttggct
     3241 ggcaggtgcc tcgacctccg gctcactgag tacgtttagc tgggcggcaa cgggatatgg
     3301 atacgataat caggcggtgg gtgaaacccg tctgacgctg ccgcttgggg gggcgatcaa
     3361 cgttaacctg caaaacatgc tggccagtga cagctcatgg agcaacatcg ccagcatcag
     3421 cgccactcta cctggaggct tcagttcgct gtgggttaac caggaaaaaa cccgcattgg
     3481 caatcaattg cgacgtagcg atgccgacaa ccgtgcaatc ggcggcacac tcaacctgaa
     3541 ctcactgtgg tcgaagctgg gtacgttcag catcagctac aatgatgacc gccgctacaa
     3601 cagccattat tacacggcag attactatca aagtgtctac agcggtacct ttggttcgct
     3661 tggcctgcgg gccggtattc agcgctataa caacggcgac agcagcgcca atacagggaa
     3721 atatatcgct ctcgatctct cgctaccact gggcaactgg tttagcgcag ggatgaccca
     3781 tcaaaacggc tacaccatgg caaacctgtc agcacgcaaa cagtttgatg aaggaaccat
     3841 tcgcactgtt ggtgccaatc tgtcacgagc catctccggc gataccggtg atgacaaaac
     3901 cctcagcggt ggggcgtatg cacagttcga cgctcgttac gccagcggaa cgctgaacgt
     3961 caatagcgcg gcggacggct acatcaatac taatttgacc gccaacggca gcgtcggctg
     4021 gcagggtaaa aacatcgctg ccagcgggcg gaccgatggc aacgctgggg tgatattcga
     4081 cactggcctg gagaacgatg gtcagatcag cgccaaaatc aacgggcgga ttttcccgct
     4141 taacggcaag cgtaactatc tcccgctctc tccctatgga agatatgagg tggagttgca
     4201 gaacagcaaa aactcactcg acagttacga tatcgtcagc ggtcgcaaaa gtcatctgac
     4261 tctctatcca ggcaatgtcg ctgtcattga gccagaggtg aagcagatgg ttaccgtctc
     4321 cggtcgtatc cgtgcggaag acggcacact gctggctaac gcacggatta acaaccatat
     4381 cggccgaacc cgaaccgatg aaaacggcga gtttgtcatg gacgtggaca agaaataccc
     4441 cactatcgat tttcgctaca gtggcaataa aacctgcgaa gtggcactgg aactcaacca
     4501 ggcgcgcggt gccgtctggg tcggtgatgt ggtctgcagc ggcctctcat cgtgggcggc
     4561 ggtgacgcag acaggagaag agaatgagag ttaacctact gatagcgatg ataatcgttg
     4621 cgctaatctg gcctgcaacc gcgctcagag cggcagtgag caaaacaacc tgggcggatg
     4681 caccggcacg cgagtttgtg tttgtcgaaa acaactcaga cgacaacttt ttcgtcactc
     4741 ctggcggggc gctggatccg cgcctgaccg gtgccaaccg ctggaccggt ttaaaataca
     4801 atggttcagg aaccatctat cagcaaagtc tcggctacat tgataacggt tacaacaccg
     4861 gcctttatac caactggaag tttgatatgt ggctggaaaa ttcaccagtt tcatctcctt
     4921 taactggctt gcgctgcatc aactggtacg cagggtgtaa tatgaccacc agtcttatcc
     4981 tgccgcaaac caccgacgcc agtggatttt atggcgcgac ggtgaccagc ggcggcgcga
     5041 agtggatgca cggcatgttg tcagacgcgt tttaccagta tctgcaacaa atgcccgtcg
     5101 gcagcagctt tacaatgacc atcaatgcct gccagacctc tgtgaactat gacgccagta
     5161 gcggcgcacg ctgtaaggat caggcctccg gcaactggta tgttcgcaac gtcacccata
     5221 cgaaagcagc aaatctgcgg ttgataaata cccactcgct ggcggaggtg tttatcaaca
     5281 gcgacggagt accgactctg ggcgaaggga acgccgactg ccggacgcaa accatcggca
     5341 gccgttcagg attaagttgt aagatggtta actataccct gcaaacaaac ggactcagca
     5401 acacctcaat ccatatattc ccggcgatcg ccaactcgtc gttagcctcg gccgtcgggg
     5461 cgtacgatat gcagttcagt ctgaatggca gttcatggaa accggtgagc aatactgcct
     5521 attactacac cttcaacgag atgaagagcg cagactcgat ctatgttttc ttctcgagca
     5581 acttctttaa acagatggtg aacctcggga tcagcgatat caacaccaaa gatctattca
     5641 actttcgctt tcagaacacc acatcaccgg agtctggctg gtatgaattt tctacctcca
     5701 acacgctgat tatcaaaccc cgtgatttca gcatcagtat tatctccgat gaatatactc
     5761 agacaccgtc gcgggaggga tatgtcggca gcggcgagtc ggcactcgat ttcggctata
     5821 tcgtaaccac cagcggtaaa acagctgccg acgaagtgct gatcaaggtg accggacccg
     5881 cgcaggtgat tggcgggcgc tcctattgtg tcttcagctc cgatgacggt aaggcgaaag
     5941 taccgttccc ggcgacgctt tcctttatta cccgcaacgg agctacaaaa acctacgatg
     6001 ccgggtgcga tgatagctgg cgggatatga ccgatgcgct gtggttgacc acaccgtgga
     6061 ctgatatctc tggcgaagtg gggcagatgg ataagaccac agtcaaattt tcgattccaa
     6121 tggataacgc catttctctg cgtacggtag atgataatgg ctggtttggc gaagtcagcg
     6181 cttcaggaga gattcatgtt caggcgacgt ggcgtaacat taactaaggc cctgctgaca
     6241 gtggtctgta tgctggcggc acctttgaca caggcgattt cggtcggcaa tctgactttt
     6301 tcactgccgt ccgagactga ctttgtcagc aaacgtgtcg tgaataacaa caaaagcgca
     6361 cggatatacc gtattgccat cagtgctatt gatagcccgg gcagcagtga attgcgcacc
     6421 cgaccggtgg atggtgaact acttttcgcc ccccgccagc tggcgttgca ggctggtgag
     6481 agtgagtatt ttaaatttta ctatcatggt ccacgggata accgcgagcg ctactaccgg
     6541 gtctcatttc gcgaggtccc cactcgtaac ctgacaaaac gcagcccaac cggcggtgag
     6601 gtcagcacgg agccggtggt ggtgatggat accattctgg tagtacgacc tcgtcaggtt
     6661 cagtttcaat ggtccttcga tcaggtgacc ggagcggtaa gtaacactgg caacacaggt
     6721 ttaagctact gattaagcca ggatgtgatt cgaccgaaga ggaaggcgat gcctggtatc
     6781 tacgtccggg agacgtggtt catcagcctg agttacgtca gccggggaat cattatctgg
     6841 tctataacga caaattcatt aagattagcg attcctgtgc ggctaagccc ccttcggcgg
     6901 actaagactt atccatcgga cgagggaaga actccacctc agagactcaa ataatcgttt
     6961 aaatgatgtg gaagcgatcg tcaatgtcac ctgttatgat gggctttctc atatgtacac
     7021 catagtgttc aggagacacg gaaaggtgcc aggctacctg ctaacgagtg gtttcttcac
     7081 taattaggac gtaaaatcgg taacggctgg aaatcattca atactcacac tatcgaaagt
     7141 acaccagcca accgcggtac gttcttacat acgatgtacc gctgttctct ttacgattta
     7201 tagctgtact ggtgaattat gagcaatctg aatccatgca tgacgtgtgg tgcctgttgt
     7261 gcatttttcc gcgtctcttt ttactgggcc gaagccgacg atgctggcgg taccattccc
     7321 gccaggttca ctgaacaaat atcccctttt caccgatgca tgagcggtac caatcagaaa
     7381 aacccccgat gtattgccct tgcaggaaca ccgggcaaaa atgcctgctg cacgatatat
     7441 aaaaatcgat cgtccacgtg cagagaattc gccatgtctg gtgagaacgg agtcgtcaat
     7501 gaggcttgca atcgtgcaag agctaaatac gggctgacac cgttataaat atacaataat
     7561 taattgcact gccccgccag ctataatcgc ggggcttcgt ttttcaggta taacaaaacc
     7621 cgaaacttct tccatctgcc agcaacaatg cctcttcctc tatagcgttc tccggatcta
     7681 cagcatcctg aagtagcgcg atgtactcac tggctatctg catcagaaag ttaaaaacaa
     7741 tgtttttaac ctataaaaat ggcgctatat ttgcgccatt tttatcattc aaagcattat
     7801 ctatttgagc ctaaagggat ctcaggatct ggctcatggg taattctgtt tcgtaaatct
     7861 ctgcgaataa tttcaataga ccagaaccag actaaatgtc caaaaatttc agagacattc
     7921 tcataccacg gaagatcaaa gagaggtggc gtaagtccca tgagagggaa tgaaatcata
     7981 tgaacaaaaa gttgcgctaa agcacctgcc agtaagccct gccaaagttt aattttcgga
     8041 aatacttcgg cgaccacaca gtaaccaacg gcaaaaacta tcgaaaagat tatgtgcgtt
     8101 acaccaaccc agttaaagac atgcccggcg aaggtataaa cagccgaatt tggatctgtc
     8161 agtcccaacc aatcccgaag aaaaatgtac ggaggattga gaaagttacg ggagcaatca
     8221 atttggcctg cagccctgat taatgattcc ggaccacacg ctgcattaaa catatcaacc
     8281 ggactacgtg gtggcaatgg aacttctgcc ccccacttaa caaatgcgga aacaacgcca
     8341 gcaatcagcc caatgaatgc agcaagacca taacgtctgc ggtttggtgg agtttgttca
     8401 aatatattca tatctaccct gcttgtacca ttatgttata cacctcttta ggagtattca
     8461 taaaacaagg caaatgtaaa gaactgtatt gttttgtata accacatggt tacctaatcg
     8521 ccaattaata taagctccat tatttctcca tatttttatg ctagaagtga caaggagaaa
     8581 tgtaatgttt ctctcagaga atataaatca ttccttttgc ccaccttaat ggtgggcttt
     8641 tttatgcaaa acctgaacgt cctgtggtat tcctctggca taatgatttt cgcgattaac
     8701 caacaaccag accagcaaat acgccaccac cagcacagca acatccatca ggtttgccac
     8761 atcccatgcg tgcggctcaa agccgccatg gcatattaat ccgcttgcca ttttcgaaca
     8821 gctgataggt ttagtccggt gtttccctgc ttgcacttaa aaatgagcgg atgggaaact
     8881 ctcgaaatgg ttcgtcgata tgctcacctc gcacctaatc accttaccga acacgcacgg
     8941 taaatagatt cgatcctgaa cccatcggtc ccaaatctgt cccaatcaaa aaataaggaa
     9001 ggtattaatg atgtgtaact tattgattta aaatggataa tataaacaga ttgtaaaaag
     9061 caagacattg atatgtatat aaaaaaacta ttacgcaatc attagatacc cccagattta
     9121 gttgtaaagc aagcacactt tgcatttcga gacaatccag gtaaagactc ctacctccca
     9181 ttttgtgatt tttatactta tgatgataag ttgaacgttg ggtagtgctt ccaatcagta
     9241 gaacatgtgc ttttcaatga aggaaccgat gactttttcg tggattgcaa cggaacgcga
     9301 gaagcagatt gttttactta ccagccgctt gatgcgggtg cggagggtca ggttgttacg
     9361 ttcaatacgt tgcgtgaaga ttttgccggt caggtatttc tccttcggca cttctctggc
     9421 gtagctgccc cagtcatcgc ggccaccatg ccgatacaga acggagtcaa cagggctaac
     9481 aattcccgac aggcctcatc attccgtggc ccgaaagtgt aagccaacac accgccagtt
     9541 ttagtgttct acgcatacca gagccagtgt tgttgcgcct tgttgccaac gaagctccac
     9601 agctcatcaa gttcacagat aagggcaaca tcagcgtgag ctgactgacg cagatgttat
     9661 ccgctggggc gagagctttt taatgtccgt atgacggtat tgatgctgat tttcagtgtt
     9721 ctggcgatat cacggcccct gtgccattat gcgccatttc aacgatcagc tccttaaagc
     9781 cgggttttct ggcttcataa gagtaagtga gctggaaaac acgtttacat gaacgacaac
     9841 ggaaacgctc atgctgggaa caactacgac catgtcgata aactttatcg gaattacagc
     9901 gcggacaatg aacagtaacg gtagccatgg aaaaccttaa ataccagtag attaccagaa
     9961 acttctatca attgaagaca ttaccgtttt tgattacagc aaatgtttta ttaattaccc
    10021 caccatcgac taaatccgtt agaacgtcga atgatgccat tcgtaaatac attaaaaaga
    10081 ctgttgtaat caacgaagat ggaaaacagt gtgttaaaac taatctgaac ttcagaaaaa
    10141 cagatgatgg tatggtgata tatatatccg ctctgaaccg ccacgcaatt taaaataaaa
    10201 atcaacaaat atcgtcgata tcca
//
LOCUS       DAPHCV010000143        10209 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.144, whole genome shotgun sequence.
ACCESSION   DAPHCV010000143 DAPHCV010000000
VERSION     DAPHCV010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.144"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(61..867)
                     /gene="trpA"
                     /locus_tag="RH033_004061"
     CDS             complement(61..867)
                     /gene="trpA"
                     /locus_tag="RH033_004061"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HDV2993172.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLDHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLVALKVFVQPMKAATRS"
     gene            complement(867..2060)
                     /gene="trpB"
                     /locus_tag="RH033_004062"
     CDS             complement(867..2060)
                     /gene="trpB"
                     /locus_tag="RH033_004062"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HDV2993173.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETDVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMHENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2072..3433)
                     /gene="trpCF"
                     /locus_tag="RH033_004063"
     CDS             complement(2072..3433)
                     /gene="trpCF"
                     /locus_tag="RH033_004063"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HDV2993174.1"
                     /translation="MMQTVLAKIVADKAIWVETRKEQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLS
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEASNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLNAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(3434..5029)
                     /gene="trpD"
                     /locus_tag="RH033_004064"
     CDS             complement(3434..5029)
                     /gene="trpD"
                     /locus_tag="RH033_004064"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HDV2993175.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDSQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5029..6591)
                     /gene="trpE"
                     /locus_tag="RH033_004065"
     CDS             complement(5029..6591)
                     /gene="trpE"
                     /locus_tag="RH033_004065"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HDV2993176.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLAAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTETAPPLPVVSVPHMHCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6613..6706)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6683..6727)
                     /gene="trpL"
                     /locus_tag="RH033_004066"
     CDS             complement(6683..6727)
                     /gene="trpL"
                     /locus_tag="RH033_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HDV2993177.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            6865..7746
                     /gene="yciV"
                     /locus_tag="RH033_004067"
     CDS             6865..7746
                     /gene="yciV"
                     /locus_tag="RH033_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HDV2993178.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAISPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7743..8363
                     /locus_tag="RH033_004068"
     CDS             7743..8363
                     /locus_tag="RH033_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDV2993179.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            8391..>10209
                     /locus_tag="RH033_004069"
     CDS             8391..>10209
                     /locus_tag="RH033_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HDV2993180.1"
                     /translation="MAGIYRCILLLIVGLFFSSLSYPKNTEIPFYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNKWPF
                     EIYKTRFSLKFPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLGNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLACGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEDQYDKNQQILIRV
                     NEGATRNNAVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRRAFLRMQKFYLPRKKSSF
                     YRPDTFLQWGGMAILAVILYGNLSPVGWAGMSLVGDMFIMICWLLPFLFCSLELLFAR
                     DDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVFLTGYLTGI
                     GYIFLPKFTQTGQQRYAHGEAIVNYLAGKEAATHSGRRRKGETRKLDYALLGWAVSAN
                     LGREWAARITPSLTAAVRAPEIARSGVLFSLQMHLSLGAN"
BASE COUNT         2372 a         2651 c         2701 g         2485 t
ORIGIN      
        1 atttttccgg cttcgttaaa gaaagttaaa atgccgccag cggaactggc ggctgtggga
       61 ttaactgcgc gtcgccgctt tcatcggttg tacaaaaact ttcagtgcca ccagcatttt
      121 ctctggctca ttaatatgtt gctcgatgat tttaacaatg gctgaacctg aaatcgcgcc
      181 tgcagctcct gcatcaatcg ctgctttgac ctgatccggg gcggaaatac caaatccctg
      241 caatggaggt gcagcgttgt actctttcag cttcgcaacc agatgatcga ggggtaacgc
      301 ggcgcggttt tctgcgccgg tcacgcccgc acgtgacaat aaataggtgt atccacgacc
      361 gtaggaggct atctggcgca gcaaatcatc gtcggcattc ggcgggcaaa taaagatagg
      421 tgcgacatta tgacgcaacg cggcctggcg gaagggagcg gactcttcca ctggcacatc
      481 ggcaaccagc accgaatcga cgccgacttt ctcgcactgg gcataaaact catcaatgcc
      541 tttgttaaac accaggttgg catacatcaa aaggccgatg ggaatggtcg ggtgcttctg
      601 gcgaatcagt gccagcatct caaagcactg tgccggggtc acacctgccg caaaggcacg
      661 cagtgtggcg ttttgaatcg tcgggccatc cgccagtggg tcggagaagg ggatacctaa
      721 ctccagcgcg tcagcaccgg cttcaattag cgtgtcgata attttcagcg actgctcaat
      781 gcccggatca ccgagcgtga cgaaaggaac gaatgcgcct tctttgcgct ccttcaactg
      841 ggcaaacaga gattcgtagc gttccatcag atttcccctc gtgctttcaa aatatcgtga
      901 acggtgaaga tgtctttatc gccgcgaccg gaaaggttaa ccaccagtag ctgctctttt
      961 tccgggtttt cgtgcatcat tttcagcgca tgggccaggg cgtgggagga ctccagcgcc
     1021 gggataatcc cttcgtgcag gcacagcgtt ttgaaggctt ccagtgcttc atcatcggta
     1081 attgacacgt aatcagcgcg tccagtgctg ttaagatacg cgtgttgtgg gccgacagac
     1141 gggaaatcca gtccggcgga gatggagtaa gattcttcaa tctgtccgtc ttcggtttgc
     1201 atcatcggcg ctttcatacc gaaatagatg cccacgcgac cgtgttttaa cggtgcgccg
     1261 tgctcgccag tttcgatacc gtgaccgcct ggttcgacac caatcaggcc gacgtcggtt
     1321 tcgttgataa aatcagcaaa catgccgatg gcattcgaac cgccgccaac acaggcgata
     1381 accgcatccg gcaggcgacc ttctctttcc agaatctgcg ctttcgtttc ttcgccaatc
     1441 attcgctgaa actcacgcac aatggtcggg taaggatgcg ggccagccgc ggtgcccagc
     1501 atatagtgcg cggtttcata actgccggac cagtcgcgta gcgcctcatt acaggcatct
     1561 ttcagcgtcg cggaaccgct atgcaccggg atcacttccg cacccattaa acgcatacga
     1621 aaaacgttag gcgactggcg ttcaacgtct ttggcaccca tataaatgcg acatttcagg
     1681 ccgagcaggg cgctggcaag ggccgacgcc acgccatgct gaccggctcc ggtttcggcg
     1741 atgatttccg ttttacccat ccgcttcgcc agcaacgcct ggccgagcac ctggttggtt
     1801 ttatgcgcgc cgccgtgcag caaatcttcg cgcttgagat acagcgtggt gttagtgcca
     1861 gccgtaatgt tctggcattt ggtgagtgcg gttggacgcc cggcatagtt tttcagcagg
     1921 tcgttgaact gagcctgaaa ttcaggatct ttttgcgcgc taacaaaagc ttcttccagc
     1981 tggcgcagag caggcatcag gatttgtggc acgtacatgc cgccaaactc accaaaatac
     2041 gggttaagta atgttgtcat tgttcctttc cttaatatgc gcgcagcgtc tggaaaaccg
     2101 aggccaaaag acgtgcgtct ttgatgcccg gttgcgactc tacagcagaa ttaaaatcaa
     2161 gtccggcgca gccggtttgt gccgcttcca cgcagttatc tgcgcctaag ccccccgcca
     2221 gcagaacgtt gccaagcgat tgaccgttta acagtgacca gtcgaaacgt tgaccgcttc
     2281 caccctggcc gttgtctaaa acatatttat caacgtgctg aagctcgcgg gcgggcaggg
     2341 tttcaccgac gcttaatgct ttccagatgg caacgtgtgc tggcagagct tcacgcagcg
     2401 tatcgatata cagctgatct tcattaccat gcagttgcac tgccgccagc gataacacct
     2461 tagctttgtc caccacatcg gcaatatcgt gattgcggaa cacgccaaca tactgcaacg
     2521 gtgccgcagc catcacttcc tgcgcctgtt caacgttgac gcaacgcggt gatgtcgcaa
     2581 caaaaatcaa cccaccgtaa atcgcgcccg cgtcataagc tgctttagca tcttgcccac
     2641 gcgtcaggcc acatacttta ttctcaccca gcaacactcg gcgcacggcg gcgttcaaat
     2701 cgtcatgggc catcaacgcc gagccaatta aaaagccgtt agcgaagtgg cttaactcgc
     2761 gcacctgagc gtaagtattg atgccggatt cgctgattac cgtcacgttg tgccccagtt
     2821 tcggcgcaag ctcgcgggta cggttgagat caatcgacaa atcgcgcaga tcgcggttgt
     2881 tgatgccaac gacctttgcc cccaatgcaa tggcgcgctc cagttcctct tcattactgg
     2941 cttcggtcag cacgcccatc tccagactgt gggcgacggc ggcaagctgg cggtattgtt
     3001 cgtcatccag tactgaaagc attaataagc aggcatcggc ctggtaatag cgcgccagat
     3061 agatctggta agggtcgata ataaagtctt tacataaaat cggctgcggg gcgatttggc
     3121 tgacgatgga gaggaaatca aagctcccct gaaaatattt ctcatcagtc agcaccgaaa
     3181 ttgccgaagc gtaatgttta taaatggcgg caatgcgtgc cggatcgaaa tcatcacgga
     3241 tcacgccttt tgacggcgac gcttttttac actccagaat aaacgccgtg cgtgcaccct
     3301 gaagtgcatc ataaaaatgt cgcgtgctcg gctgaacctc attctgaaaa ctggccagcg
     3361 gttgctgctc tttgcgggtt tctacccaaa tcgccttgtc tgcgacgatt ttcgctaaaa
     3421 cggtttgcat catttaccct cgtgccgcca gtgcggtgac tctgtcgtaa gcggaaccac
     3481 tgcgcagtac ctcaagaacg gtttgcgcat tggcttgcag atcttcatgg ccatgcaggc
     3541 gcattaacat ggcgacgttc gccgcgacgg ctgcttcatg ggcggcgtcg cctttacctt
     3601 gtaacaagcg tgttaaaatg tcacggtttt cttccggtgt tccgcctgcc agttgctcct
     3661 ggtggtaggg tgtcaggcca aaatcttctg cagttagctg atagctctta atttcaccgt
     3721 catgcagttc ggcaacgatt gtcggcgcgt gtaatgaaac ttcatccatc ccgccgctgt
     3781 gcactaccgc agcgcgttga taccccagca cgcgcaaggt ttcggcaatc ggcagcacca
     3841 gttccggact ataaacacca attagcgcca gcggcggatg cgccgggtta atcaatggcc
     3901 ccaacacgtt gaacagggtg cgggttttca gttgctggcg aaccggcatc gcgtggcgga
     3961 atccggtgtg atatttcggc gcaaagagga aacagacgcc taactcatcc agcgcctggc
     4021 gcgatttatc ggcgttcata tcaagattaa taccgaacgc cgccagcaga tcggacgagc
     4081 cggatttact ggagacgctg cggttaccgt gtttcgccac tttcagccca caggccgcgg
     4141 cgacaaacgc actggcggta gaaatattga tgctgttgct gccgtcaccg ccagtaccga
     4201 cgatatcggc aaacagataa tccgggcgcg ggaacggcgc ggcgttttcc agtagcgcgg
     4261 ttgctgctcc ggcgatctcg ttcgggtgct cgccgcgaat tttcatgctc accagcgccg
     4321 ccgccagttg ttccggcttc agctcgccac gcaccaccgc tgaaaacagc tggtgacttt
     4381 cttgttggct aagcgtctgt gcctgataca gtttttccag aatcggttgc agcgtgttgg
     4441 ttggctcaag tttctgctgc gcccaggcca gcgtttgttc cagcaggcga gcgccctggg
     4501 tagtaagaat ggattccgga tggaactgga aaccgcaaac gcgatctgca tcgtgacgta
     4561 ccgccatcac catgccatta aaatgggcgt tgatggttaa acctgccggg atattactgc
     4621 caaccagcga gtgataacgc gccaccggca atgggtttgt taatccggca aacatcgcct
     4681 gactgtcatg ttcaatgctc gacgctttac cgtgaagaat ttcgcccgcc tgaccgacat
     4741 agcccccgta agcttcgaca atcgcctgat gaccgaggca aatgccaata attggcagct
     4801 tgccgcgcag gcgggtgagg agttctggca tacaaccggc ttcgcttggc acaccggggc
     4861 caggagaaag catcagcacc ggattgctca ttgtcgccag gcgttcaatt aaggtctgcg
     4921 ccggaatatg gttgcggtaa atcaccacgt tatgaccatt gctgcgcaac tgatctgcca
     4981 ggttgtaagt aaaagagtcg atattatcga gcagcagaat gtcagccatc agaacgtctc
     5041 ctgtgcatga tgcgcggtgg caatagcgcg cagtacagcg cgggctttat tacgggtttc
     5101 gtcggcttcc gactgcggaa cagaatcaag taccacgcca gcaccggctt gcacggtggc
     5161 gataccgttt tccaccagtg ccgagcggat cacaatgcag gtgtcgagat cgccatgcgc
     5221 ggtgaaataa cctaccgcgc cgccgtagct gccgcggcga cgaccttccg cctcggcaat
     5281 taactgcata gcgcgtactt tcggtgcacc gcttaacgtc cccatattca tacaggcgcg
     5341 gtaagcgtgc agggcgtcga gatcgtggcg cagctcaccg accacgcggg agaccaggtg
     5401 catcacgtaa gagtaacggt caactttggt gagatcggcg acgtagcggc tgccgggggt
     5461 gcaaatgcgt gccagatcat tacgggcgag atccaccagc atcagatgtt cagaaagctc
     5521 tttatgatcg gtacgcattt ccagttcgat gcggctgtcg aggtctctgt ccagcgaacc
     5581 atcggcacga cgaccgcgtg gacgtgttcc ggcaatcggg tagatctcaa tctggcggct
     5641 ggtggcgtca tacttgagcg agctctccgg cgacgcgcca aacagggtga aatcattatc
     5701 ctgcataaaa aacatatacg ggctgggatt actctttttc agcacgtaat aggccgccag
     5761 cggtgacggg cagggcagag agaaacggcg agatggcacc acctggaaaa tttctccggc
     5821 gcgaatcgct ttttgcaaca aacgcactac gccaccgaac tcttcatcgc tctggttaca
     5881 ttcacaatgc atatgcggca cggaaaccac cggcagcggt ggcgcggttt cggtcagctg
     5941 ctggcgaaga tcgttcaggc gagcagtgag acgttgtttt tcttcttcat tcggagcaaa
     6001 aagactggcc tgaatgcggg tgcttttttt ctgatggtca atcaccatca gcgtttcagc
     6061 gagataaaaa cagaaatcag ggcagttatt ttccgctgcc agttgcggta aatcttcaaa
     6121 ccccgccaca aggtcataag agaacaggcc gccgaagaac attgcttctc gttcttcctt
     6181 cggtacattc aacagattct gcaataagcg gaaagcgtca aaaaccgaaa gggagcataa
     6241 gcgggcgtct tcatccagca gtggactgac aggggggaag cgcagcacac ggcagtttgg
     6301 tgattgttca ttttccacac ccgcaggcag ggcgttatcc agtagtgcca acagggcttc
     6361 gccgttgccg gaaagtgcct gaattgtgac agtgtcacct aatgctgtaa tgcgcagcgc
     6421 actgtctacc agcagcaggc tttttaaatc atctttgctg tcgatatctg cggattccag
     6481 cagcagcgtt gctggacgat tcccacacaa ctggtgaaaa agcgcagtcg ggttgtcgcg
     6541 ataagcgcct tcgcaggtta gcagttcaag agtcggtttt tgtgtttgca ttgttattct
     6601 ctaattttgt tcaaaaaaaa gcccgctcat caggcgggct gggtatctga ttgctttacg
     6661 catggtgaat acactgcccg tttcaggaag tgcgccacca acctttcagt acgaaaattg
     6721 ctttcattgt cgataccctt tttacgtgaa cttgcgtact agttaactag ttcgatgatt
     6781 aattgtcaac agctcatttc agaatatttg ccagaaccgt tatgatgtcg gcgcaaaaaa
     6841 cattatccag aacgggagtg cgccttgagc gacacgaatt acgcagtgat ttacgacctg
     6901 cacagccata ccacagcttc cgatggctgc ctgacgccag aagcattggt gcaccgtgca
     6961 gtcgagatgc gcgtcggcac cctggcgatc accgaccatg acaccacagc tgcaatctca
     7021 ccagcaagag aggaaatttc acgttcggga ctggcgctga atcttattcc cggcgtggaa
     7081 atctccacgg tctgggaaaa tcatgaaatt catattgtcg ggctgaatat tgatattact
     7141 catccgctaa tgtgtgagtt cctcgcgcag cagacagaac ggcgcaatca gcgggcgcag
     7201 ctgattgccg aaagacttga aaaagcgcag ataccaggtg cgctggaagg cgcgcaacga
     7261 ctggcgcagg gcggtgcggt gacgcgcggt cattttgctc gtttccttgt ggagtgcggc
     7321 aaagccagtt caatggcgga tgtctttaaa aaatatctgg cgcgcgggaa aaccggatac
     7381 gttccgccac agtggtgtac aatagaacaa gctattgatg tcattcatca ttctggcggt
     7441 aaggcggtgc tagctcatcc tgggcggtac aatcttagtg ctaaatggct gaaaagattg
     7501 gttgcgcatt ttgctgaaca ccacggcgac gcgatggaag tcgcgcagtg ccagcaatcg
     7561 cccaatgaac gcacccagct ggcgacactt gcgcgtcagc atcatttatg ggcatcacaa
     7621 ggatctgatt ttcatcagcc gtgcccgtgg attgaactgg gtcgtaaact ctggctgcct
     7681 gcaggcgttg aaggcgtctg gcagctatgg gaacagccgc agaacaccac agagagggaa
     7741 ttatgagcca gtttttttat attcatcctg ataacccgca gcaacgtctg atcaaccaag
     7801 cggtggagat cgtgcgtaaa ggcggggtga ttgtttatcc aaccgattcc ggctatgcgc
     7861 tcggctgtaa aattgaagat aaaaacgcca tggagcgtat ttgtcgaatt cgccagctgc
     7921 cggacggtca caactttacc ctgatgtgtc gcgatctttc tgagctctcg acctattcat
     7981 tcgtggataa cgttgcgttt cgtttgatga aaaacaacac gccgggcaac tacaccttca
     8041 tcctgaaagg gacgaaggag gtgccacgcc gtctgttgca ggaaaaacgc aaaaccatcg
     8101 gtatgcgtgt gccttctaac ccgattgcgc aggcgttgct tgaagcactg ggcgaaccga
     8161 tgctttccac ttcgctaatg ctgccaggca gcgaatttac cgaatccgat ccggaagaaa
     8221 ttaaagaccg tctggaaaaa caggtggact tgattattca cggcggctat ctcgggcaga
     8281 aaccaacaac ggttattgat cttactgatg atacgccggt cgtggtgcgt gaaggcgtag
     8341 gtgatgtgaa gcctttctta taacaaattg cgttccacgg atggaagact atggcaggga
     8401 tatatcgctg cattttgctg ttgatagttg ggcttttttt ctcttcgttg agttatccga
     8461 aaaacacgga gatccctttt tatgaagaag ggatctcgct ctttgatgtt gaagccactc
     8521 tgcaaccgga tggggtgctc gacatcaaag aaaatattca ttttcaggcg cgaaatcagc
     8581 agattaagca cggattttat cgtgatttac cacgactctg gatgcagcct gatggggacg
     8641 ctgcactgct gaactatcat attgttggcg tcacccgtga tggtattcct gaaccctggc
     8701 atcttgactg gcatatcggg ttaatgagta ttgtcgtggg cgataaacaa cgtttcttgc
     8761 ctcaaggcga ctatcattat caaattcatt atcaggttaa aaatgctttc ctgcgtgagg
     8821 gagattctga tctgttaatc tggaacgtga ccggtaacaa gtggccgttt gaaatctata
     8881 agacccgatt ttcactcaag ttccctgata tcgcgggtaa tccatttagc gaaatcgatc
     8941 tctttactgg agaagagggc gacacatatc gaaatggccg catccttgag gacggaagaa
     9001 ttgaatcccg cgatccgttt tatcgtgaag acttcacggt cctctaccgc tggcctcacg
     9061 ctttacttgg taatgccccg gcaccacaaa cgacgaatat tttcagccat cttcttttac
     9121 cctctacgtc atcgttgtta atttggtttc cgtgtctgtt cctggcgtgt ggatggttat
     9181 atctctggaa gcgcaggcca caatttacgc cggtagatgt gattgaaacc gatgtcattc
     9241 cgccagatta cacgcccggc atgttacgtc tcgatgcgaa actggtttac gacgataaag
     9301 gtttttgtgc cgatatcgta aatctgattg tgaaaggaaa aattcatctg gaagatcagt
     9361 atgataagaa ccagcaaatc ctgattcgtg ttaatgaagg cgcgaccaga aataatgcgg
     9421 tattactgcc cgcagagcag ttattactgg aagcgttatt tcgtaaaggc gataaggtcg
     9481 ttcttacggg gagacgcaac agagtcttac gcagggcatt tttacggatg cagaaatttt
     9541 atctgccgcg taaaaagtct tcgttttacc ggcctgatac gtttttgcaa tggggcggaa
     9601 tggcgatatt ggcggtcatt ctctatggta acctgagtcc cgtaggttgg gcaggaatga
     9661 gtctggttgg cgatatgttt attatgatct gctggcttct tcctttttta ttttgttccc
     9721 ttgagctttt gtttgcccgc gatgatgaca agccttgcgt taatcgtgta atcatcactt
     9781 tgtttttacc gctgatttgt tcaggcgtgg ccttttactc tctctatatc aatgtcggag
     9841 atgtattctt ttactggtat atgccagcgg gttattttag cgctgttttc ctgaccggtt
     9901 atctcactgg catagggtat atttttctgc caaagtttac ccaaactggg cagcaacgtt
     9961 atgcccacgg tgaagctatc gttaactatc ttgcgggtaa agaggcagca acacacagtg
    10021 ggcggcggcg gaaaggggaa acacggaaac tggattacgc gttgttaggt tgggcggtct
    10081 cggcaaacct tggcagagaa tgggcagcac gtatcacccc atcactcaca gcggctgttc
    10141 gcgccccgga aattgcccgt agtggcgttt tattctcatt gcagatgcac ctgagtctgg
    10201 gggccaata
//
LOCUS       DAPHCV010000144        10120 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.145, whole genome shotgun sequence.
ACCESSION   DAPHCV010000144 DAPHCV010000000
VERSION     DAPHCV010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.145"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            186..689
                     /locus_tag="RH033_004070"
     CDS             186..689
                     /locus_tag="RH033_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HDV2993181.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(767..1018)
                     /locus_tag="RH033_004071"
     CDS             complement(767..1018)
                     /locus_tag="RH033_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HDV2993182.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1133..1219)
                     /gene="azuC"
                     /locus_tag="RH033_004072"
     CDS             complement(1133..1219)
                     /gene="azuC"
                     /locus_tag="RH033_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HDV2993183.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1482..1805
                     /locus_tag="RH033_004073"
     CDS             1482..1805
                     /locus_tag="RH033_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HDV2993184.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAYTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            1977..2474
                     /gene="ftnA"
                     /locus_tag="RH033_004074"
     CDS             1977..2474
                     /gene="ftnA"
                     /locus_tag="RH033_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HDV2993185.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(2512..2751)
                     /locus_tag="RH033_004075"
     CDS             complement(2512..2751)
                     /locus_tag="RH033_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HDV2993186.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            2942..4153
                     /gene="tyrP"
                     /locus_tag="RH033_004076"
     CDS             2942..4153
                     /gene="tyrP"
                     /locus_tag="RH033_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HDV2993187.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQLATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(4203..4868)
                     /gene="yecA"
                     /locus_tag="RH033_004077"
     CDS             complement(4203..4868)
                     /gene="yecA"
                     /locus_tag="RH033_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HDV2993188.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALGAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(5064..5150)
                     /locus_tag="RH033_004078"
     tRNA            complement(5064..5150)
                     /locus_tag="RH033_004078"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5114..5116),aa:Leu,seq:taa)
     gene            complement(5163..5236)
                     /locus_tag="RH033_004079"
     tRNA            complement(5163..5236)
                     /locus_tag="RH033_004079"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5202..5204),aa:Cys,seq:gca)
     gene            complement(5291..5366)
                     /locus_tag="RH033_004080"
     tRNA            complement(5291..5366)
                     /locus_tag="RH033_004080"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5331..5333),aa:Gly,seq:gcc)
     gene            complement(5518..6066)
                     /gene="pgsA"
                     /locus_tag="RH033_004081"
     CDS             complement(5518..6066)
                     /gene="pgsA"
                     /locus_tag="RH033_004081"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDV2993189.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(6123..7955)
                     /gene="uvrC"
                     /locus_tag="RH033_004082"
     CDS             complement(6123..7955)
                     /gene="uvrC"
                     /locus_tag="RH033_004082"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDV2993190.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDESKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(7952..8608)
                     /gene="uvrY"
                     /locus_tag="RH033_004083"
     CDS             complement(7952..8608)
                     /gene="uvrY"
                     /locus_tag="RH033_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HDV2993191.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            8904..9080
                     /locus_tag="RH033_004084"
     CDS             8904..9080
                     /locus_tag="RH033_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993192.1"
                     /translation="MIKIFIGHYINAFNSTVDDTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            9067..9291
                     /locus_tag="RH033_004085"
     CDS             9067..9291
                     /locus_tag="RH033_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HDV2993193.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(9358..10080)
                     /gene="sdiA"
                     /locus_tag="RH033_004086"
     CDS             complement(9358..10080)
                     /gene="sdiA"
                     /locus_tag="RH033_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HDV2993194.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRSSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
BASE COUNT         2543 a         2540 c         2330 g         2707 t
ORIGIN      
        1 tataaacttg atgatttaag cattttctta tacccgttca gacgttattc ttatttcaga
       61 tcatcgtcag aattgactcc acgatcacat ttcggaccgg cagaaaggaa ttattctgca
      121 aacagtaatt atggtgtttt gatttatctt gcacctctcc acttctggat ataaggatat
      181 taggtatggc aaccgctgga atgcttctca aactcaactc tcaaatgaac cgcgagtttt
      241 acgcatccaa tctctacctt cacctgagta actggtgttc tgaacagagt ctgaacggca
      301 ccgccacttt ccttcgcgcc caggcacaga gtaatgtgac ccaaatgatg cgcatgttta
      361 actttatgaa gagtgtcggc gctaccccca tcgttaaagc cattgatgtt cccggtgaaa
      421 aactgaactc tctggaagaa ctgttccaaa aaacgatgga agaatacgag caacgttcta
      481 gtacgttggc acagttagcc gatgaagcga aagaactgaa tgatgattca accgtcaatt
      541 tcctgcgcga tctggaaaaa gaacagcagc atgatggcct gttgctgcaa accattcttg
      601 atgaagtgcg cagtgcgaaa cttgcgggta tgtgccctgt gcagaccgac caacatgttc
      661 tgaatgtcgt gtcgcatcag ctacattgat catcatcggc gctaatgcat tgcgccgatg
      721 aagtttttga gaaaccgctg cctcattttt ttgaagcagc ggttttttaa tgggattcgc
      781 cctgtggggt aaacttgagt tcaatcaacg cgatggcttt ttggattgcc cgcatggtga
      841 ccgggtctgc ggcggcggga tggttagtaa agtcgatatt ctttagctgg ctggacattt
      901 tttcgcgaac ttcaacgggt gcgatcacat cgagaacatc cagaatttgt ttgataacca
      961 actggcaagc aaccacatca gaaaccagtt cctgatcggc attcagcggc tgggacatcg
     1021 taaactcctg atagcatttt gaaagccgtt atagtagcgg cttcacatct tcagcgatag
     1081 tcacatccac cgtcatcagg acacaaaaaa acctgccgga gcaggttttt tgttatcgga
     1141 acatattgcc tggcggtacg tctttgaacg tcttgcaata gttattgaac atacttttca
     1201 ggattttgcg cagtttcatc gcggcactcc gaccatttgt tatacatgtg ttattgtctt
     1261 tgcgcttata atatgacaac catcacaaaa atcaatcttt atgtgatgta aatcacataa
     1321 ataccccttt aatgttataa aaatgataat caaaaagcag ctaccccatt tctgacacct
     1381 acagatggcg agaaatagcg cctgccaggc gtcttttccg gccattgtcg cagcgctgta
     1441 acgcgtaaaa tagtgctttc ttttactctt ctggctggac catgagactt ctgattctga
     1501 ctctttcatt aataacgctg gcggggtgta cggttactcg ccaggcccat gtgagcgaag
     1561 ttgacgcggc aacaggtatt gtacggttgg tttatgacca ggcttttttg cagcatgcct
     1621 ataccgatcg ttatgtcagt cgcgggatcg ccgatcgcgc atgccagcag gaaggctata
     1681 cccacgctat cccctttggt cagccagtag gcaactgtag cctctttgcg ggttccttat
     1741 gtttgaatac ggaattcact ttgtcatatc agtgccatca ttccgccttc cctgtctttc
     1801 tctaaatgtt ttaatcagcg agggggatct tcgctgatta aataaatagc ggtaatgcgt
     1861 tttattccca ttcgtatttt taataaatga agtttatatt ttaccttttg caaataataa
     1921 aataacaaat tatagtgacg ccacagcaac aaatataacc tttgtggagc actatcatgc
     1981 tgaaaccaga aatgattgaa aaacttaatg agcagatgaa cctggaactg tactcttcac
     2041 tgctttatca gcaaatgagc gcctggtgca gctatcatac cttcgaaggt gctgccgcgt
     2101 tcctgcgccg tcacgcccag gaagagatga cgcatatgca gcgtctgttt gattatctga
     2161 ctgataccgg caacttaccg cgtattaata ccgttgaatc tccgtttgct gaatattcct
     2221 cacttgatga attattccag gaaacctata aacacgaaca attaatcacg caaaaaatta
     2281 acgaactggc tcatgctgca atgaccaatc aggactaccc aacatttaat ttcctgcaat
     2341 ggtatgtttc tgagcagcat gaagaagaga aactgttcaa atcgattatt gataaattaa
     2401 gtctggcagg caaaagcggc gaaggtttgt attttatcga caaagaactc tctaccctcg
     2461 acacacaaaa ctaatgttct ccggcggcag gtttactgcc gccgtttcaa atcagtgacg
     2521 gcaaatctta ctctgatcgg tcgaaaaacc ttcttccgaa ggtataaatt tgccttttgc
     2581 tgccagaaac gctaccagct ctcccgctgt catcccttct gccgagcagg tgtgaaaacg
     2641 tgcctgttca ccaaaacgcg ctttaattgc tgcttccaga ctggcatgcg tatattgctc
     2701 gcctgattca atcatcatat ttaacacttc atgaccgtga atagagtcca tcgttcctcc
     2761 tcaaaaaaag cctagcgtag cgattgccgc ttatgaagac tttgcgccag cgcagaactg
     2821 aacgcttttt attgtacatt tatatttaca ccatatgtaa cgccggtttg acgaatcagc
     2881 cgttatgcct taacctgcgc cgcagatatc actcataaag atcgtcagga cagaagaaag
     2941 cgtgaaaaac agaactctgg gaagtgtttt tatcgtggcg ggaaccacaa ttggcgcagg
     3001 catgctggca atgccgctgg ctgcggcagg tgttggtttt agcgtcacgt taatcttgtt
     3061 gattgggctt tgggcgttga tgtgctacac ggcgctatta ctactggagg tgtaccagca
     3121 tgtcccggca gataccggtc tgggcacgct ggcaaaacgc tatctgggac gctacggtca
     3181 gtggctgacg ggcttcagta tgatgttctt aatgtatgct ctgactgcgg catacatcag
     3241 cggtgccggt gaattgttgg cctccagcat cagcgactgg acaggtattt caatgtcggc
     3301 aaccgctggc gtgctgttgt tcacttttgt tgccggtggc gtggtttgtg tcgggacatc
     3361 gctggtcgat ttatttaacc gttttctgtt cagcgccaaa ataatttttc tggtggtgat
     3421 gctggtattg ctgctaccgc atattcacaa agtgaatctt ttaaccctgc cgttgcaaca
     3481 gggactggca ctgtctgcaa tcccggtgat ttttacgtcg tttggttttc acggtagcgt
     3541 gccgagtatt gtcagctata tggatggcaa cattcgtaag ctacgctggg tgtttataat
     3601 cggtagtgcg atccccctgg tggcatatat tttctggcag ctggcgaccc ttggcagcat
     3661 tgattcaaca acctttatgg gattgctggc taatcatgcc ggattaaacg ggctgttaca
     3721 ggcgttacgc gaaatggtgg cctctccgca tgttgagctg gcagtgcatt tatttgctga
     3781 tttagccctc gccacgtcct tcctcggcgt agcgttaggc ttatttgatt atctggcaga
     3841 tttgtttcag cgttcaaata ccgttggtgg aagattgcaa actggtgcaa ttacctttct
     3901 gccgccgttg gcgtttgcac tgttttatcc acgaggattt gtgatggcgc tgggttacgc
     3961 cggtgtggcg ctggcggtac tggcattgat tatcccttcg ctgttgacct ggcaaagcag
     4021 aaagcacaat cctcaggcgg gttaccgggt caaaggtggt cgtccggcgc tggtggtggt
     4081 gtttctctgt ggtattgctg tgattggcgt gcaatttttg attgcggcag ggttgttacc
     4141 agaagtgggg tgattagatc ttattgggtg ccagaattaa cgctgacacc caatttgacc
     4201 tcttaatgca ggcagcactg cttaaatttc ttaccactgc cgcacgggca aggatcgtta
     4261 cgacccggtt tctcttctgc tttgatcggt tgctgaacag ctttttcctg cggatgcgcc
     4321 atccagtatg catgtaaatc aagcgccgcc agtcgaatgg catctacgct ctcttcaaac
     4381 gcttctggcg acatcttctc aacccgctcg aagttttcct cagtaccgtg cagcgcaatc
     4441 gcccccagcg ctggttttaa cgaatcgggc aacgttgacc agtcagaaag tgccacgccc
     4501 cgcatatagc caaagcacca ctcctcaaca atcgtcagct cgctgccatc gacttctcgc
     4561 aagccgaata acggctcaaa ctgctccggg aattcgttca gacgctctgc ggtatcggcc
     4621 atatgttgaa aagccaggtt cataaagcgc gtcatctctt tctctgacgc ccagcgcggc
     4681 acatagtcag ccccacccca cacggcaacc agccactgtg ccggttcaat ctcttgcgga
     4741 gaactcaaca ccgccgtcaa taaaccgtcc agctccgcca catcaagaat ggcgtggtca
     4801 gtgttgtatt tggtcagaat atcgtccagc cattccaact cactttcgtt taacggtccc
     4861 gttttcatac gcttttcctt gtggatctca actcgccagc acctatctta catgccggtc
     4921 cgtatcagag atactttttg agtggctttg ccggtgatta aaaattaagg agggtgtaac
     4981 gacaagttgc aggcacaaaa aaaccacccg aaggtggttt cacgacactg cttattgctt
     5041 tgattttatt cttatctttc ccatggtacc cggagcggga cttgaacccg cacagcgcga
     5101 acgccgaggg attttaaatc ccttgtgtct accgattcca ccatccgggc tcgggaagaa
     5161 agtggaggcg cgttccggag tcgaaccgga ctagacggat ttgcaatccg ctacataacc
     5221 gctttgttaa cgcgccaaat tcttcaggcc tttcagccag acatccgctt gacgccgatg
     5281 tcttttaaac tggagcggga aacgagactc gaactcgcga ccccgacctt ggcaaggtcg
     5341 tgctctacca actgagctat tcccgcattc atcaagcaat cagttaatca cttgatttta
     5401 ttatcgtctg gcaatcagtg ccgccgttcg atgcgttgca ttctacttac ctggcgcgat
     5461 gagtcaacga tatttttcac cacttttgat cgtttgctga aaattgcgcc gaaacgatca
     5521 ctgatcaagc aaatctgcac gcgcagcgct caaatattgc aacattgacc acagagtcag
     5581 taccgcagcc acaaagaaaa gtgcaatacc ggcgtactca acccaaatgt tcggacgcca
     5641 cagcagccag gccagcgcca ccatctgggc agtggttttc actttcccaa tccaggagac
     5701 agccacgcta ctgcgtttac ccaactccgc catccattcg cgtaacgcag aaataataat
     5761 ttcacgggcg atcatcgttg ctgccggtaa ggtcacccac cagctgtgat aatgctcggt
     5821 taccagcacc atggcgatag ccacgagaac tttatctgct acagggtcaa ggaaagcacc
     5881 aaaccgggta ctctggttcc agcggcgtgc cagaaaacca tcgaaccagt cagtcaccgc
     5941 cgcgacgcag aaaatgagcg cggcggcaaa cggcgaccag gtgacaggca gataaaagac
     6001 caatacaaag aatgggataa ggatgacacg gaacagtgta agcaacgtag ggatattaaa
     6061 ttgcataatg acgggtaact atctgttgtc agtaagatta cccctatgtt gctacagaga
     6121 catcaatgtt tcaacgacca gaagatcttt tctgccagac cttgcgaaat acccggcact
     6181 tttgcaattt cctcgacgct ggcgttacgt aaaccttgca aaccgcccat atatttcaac
     6241 aacatttgcc gacgttttgg cccgacgcct tcaatggttt ccagggaact ggtatttttg
     6301 accttcgccc gttttttacg gtgcccgcca atcgcgtgat cgtgtgattc atcgcgaata
     6361 tgctggataa catgcagtgc gggagaatct ggcggcaaac tgaatccctc accttccggc
     6421 tcaaagaaca gcgtttccag cccagcctta cgatctgctc ctttggcaac gcctagtagc
     6481 agcggatgat ttttatccca tgagacatcc agttcggcga aaacattttt cgcctgcgca
     6541 agttggcctt tgccgccgtc gataaggatc acatccggga tcttactctc gtcgatggct
     6601 ttaccataac gccgacgcag cacctgattc atcgccgcat aatcatcgcc cggcgtgatg
     6661 ccagtaatgt tatagcgtcg atactccgca cgcagcgggc cgttagcatc aaacaccaca
     6721 caggaagcga cggtttgttc ccccatggta tggctgatgt caaagcattc catccgcttc
     6781 acttccggca atttcaacac gctggcaagc gcggtcagtc gctggtgaac ggtagattgc
     6841 tgcgaaagtt tgctggttaa tgccgtcgcc gcattggtgc gcgcgagttt cagataacgc
     6901 gcccgatcgc cgcgaggttt ggtttgaaca ttaatcttgc gtcccgccag ttctgaaagg
     6961 gaatcggcga gcagcgtttt atcgctaaga ttaaaatcga gcaggatctc acccggtaag
     7021 gtgcgcatct ggctgccttg taaatagaac tgacctacga aggtttccac cacctcgctc
     7081 agttccgtac cgccaggcac tttcgggaaa tagctgcggc tgccgagcac tttaccctga
     7141 cgaatgaaca atacgtggac acaagccatg cccgcatcga acgccacacc aataacgtcg
     7201 aggtcgtcgc cggtattgga cacaaactgt ttttcggtga cgcgtcgcac cgcctgaatt
     7261 tggtcgcgaa tacgtgccgc ttcttcaaac tccagattct ggctggcagt ttccatacga
     7321 ctaatgagtt gcgtaagcac ctgatcatct ttgccagaca aaaacaggcg cacatactcg
     7381 acctgctgag cgtattcttc ttcactcacc agtccttcaa cgcacggtcc cagacagcgc
     7441 cctatctgat attgcagaca cggacgcgag cgattgcgat aaacactatt ttcgcactgg
     7501 cgaatgggga atatcttttg cagtagcgcc agtgtttcac gtacggcata gccattcggg
     7561 aacgggccga aatattcacc tttagcatgc ttcgcaccac gatgcatcgc cagacgcgga
     7621 tgggtatcgc cactcaggaa gataaaggga taagatttat catcgcgtag caaaacgttg
     7681 taacgcggct gatagagttt gatgtagttg tgttccagca acagcgcttc ggtttctgta
     7741 tgagtaaccg ttacatcaat ttgctggatc tgggcgacca gcgcttcggt tttgcgcgaa
     7801 gcgaggttgc tacggaaata gctggaaagc cgttttttca ggtctttcgc tttgccgaca
     7861 tagataaccg taccaccagc atcgtacatg cgataaacgc ctggctggct ggttacggtt
     7921 tttaaaaacg cttttgcgtc aaactgatca ctcactgact tgataatgtc tccgcattac
     7981 acagaccatg gcgaattgcc aggtgagtca gctcaacatc gccatgaatg tttagtttac
     8041 tgaacatacg atagcggtag ctgttcaccg ttttcggact gagattgagc tgttctgaga
     8101 tctcattgac cttctggccc ttggtgatca tcagcataat ctgcaattca cgttcagaca
     8161 aactggcaaa tgggctttct gttttttctg gttcgatctg gcttaacgcc atttgttgag
     8221 cgatgtcaga agcaatgtaa cgctgccctg aatagacaga acgaatcgca ctcacgactt
     8281 cctgcggagc cgcgcctttg ctgaggtagc ccgcagcacc ggcctgcatg actttcgctg
     8341 gtaaagggtt ttctgtatgg acggtaagca tgatgatttt gacatcagct gtggaacgcg
     8401 cgattttacg cgtcgcctca agaccgccaa tgcccggcat actcatgtcc attagcacca
     8461 cgtcaacggc atttgtccgg caccacttaa cggcgtcttc accgcacgat gcctcaccga
     8521 cgacttttat accctttata tcttccagaa tgcgtcgtat ccctgcgcgc accagttcgt
     8581 ggtcatcaac aagtagaacg ttgatcaaag gaatatctcc agaaataggg ataacgctac
     8641 tgatagttag tcattcgtat attagcggtt tttattgcaa ctttgaaacg ttaaaaatgg
     8701 tgggtattcg atttttctct cgtttttgtc aattcgattg tccacagtta tggtcacgcc
     8761 cgtcaccaac ggcttaccgg atcgtttcac agtaagccct gcgacgaaat gtgacaaaaa
     8821 ttacctttat tcagcaaaaa tgaaaatcag cccgctgaat atgtaacatt aattaacccg
     8881 aacaatacgg gcaaaaaaca ttcatgataa aaatatttat tggtcattat attaacgcat
     8941 ttaatagcac cgttgacgat acgctcaaaa aacaaccact gctattttta gcaaagctta
     9001 tggtatactc cgccgcctta acatttttca ccgcaaattt tcattgcaac atgacgagga
     9061 aaataaatga gtacgcctga tttttctact gccgagaata atcaagaact ggcaaatgaa
     9121 gtctcctgcc tgaaagcgat gctgacgctg atgctgcagg cgatgggaca agctgacgcg
     9181 ggccgcgtga tgttaaagat ggaaaaacag cttgcgctga tcgaagacga aacccaggct
     9241 gcagtatttt ccaaaacggt taagcaaatt aaacaagcct accgtcagta atataaaacc
     9301 ggctgatagc gtgcctttca gccggttttt gcatctggca cgcagaaaga aaagagatca
     9361 aattaagcca gtagcggccg cgtaacaggc aacctgggtc ttattcggtg cattgatttt
     9421 tttctgcatg tttttctgat ggaaattgac cgtattctca gagattgaca aaatcatcgc
     9481 tatctctgct gatgtcttcc cttccgccgt ccatttcaaa atttcttttt cgcgcttgct
     9541 gaaattcatc tctggcgtca tcactatttc atcatttaaa cgcatcagag ccatcagact
     9601 ttcgcgcacc agtaactgca tttttaattg cagttcatca ctaagaatgg gtatttcgcg
     9661 cgcgctgcta cgggaaaagg acaaaaagcc cagcgcccgg tttggcagca ttaaatactg
     9721 agtgacaccg cggcgtaaac catgtgcgcg cgcggcttcc cataacggct gtgcttcgct
     9781 gaataagtca tcattccaca ttaaatggcc ctgactaaag ttttcagggt tcagcaccgg
     9841 atcaattgcg agaaagtttt ttgcctgata ataactaacc cacgcctcag ggtaattggt
     9901 gtagaaagcc actttaggtc gcgtgaatgg taccgggtgg cgaacacata acgaatagta
     9961 atcgtactct agctgctgag cctgaagctc tatttcatgg tagacctctt ctgcggcctc
    10021 catcctctga aaacgcaaca gcatcgtgcg acgccagctg aaaaaatccg tatcctgcat
    10081 agtaaaccgc aacgcccctg agagtgagta tcatttataa
//
LOCUS       DAPHCV010000145         9946 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.146, whole genome shotgun sequence.
ACCESSION   DAPHCV010000145 DAPHCV010000000
VERSION     DAPHCV010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.146"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..157)
                     /locus_tag="RH033_004087"
     CDS             complement(<1..157)
                     /locus_tag="RH033_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993195.1"
                     /translation="MLQSSADKACNVLVKKVRYLNDVRKITKMMNGKLIYSWLVWPCS
                     EFFSLNYK"
     gene            235..1329
                     /gene="ddlA"
                     /locus_tag="RH033_004088"
     CDS             235..1329
                     /gene="ddlA"
                     /locus_tag="RH033_004088"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDV2993196.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1353..1565)
                     /locus_tag="RH033_004089"
     CDS             complement(1353..1565)
                     /locus_tag="RH033_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HDV2993197.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1825..2133
                     /locus_tag="RH033_004090"
     CDS             1825..2133
                     /locus_tag="RH033_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HDV2993198.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2191..3285)
                     /gene="yaiW"
                     /locus_tag="RH033_004091"
     CDS             complement(2191..3285)
                     /gene="yaiW"
                     /locus_tag="RH033_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HDV2993199.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKVSGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3298..4518)
                     /gene="sbmA"
                     /locus_tag="RH033_004092"
     CDS             complement(3298..4518)
                     /gene="sbmA"
                     /locus_tag="RH033_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HDV2993200.1"
                     /translation="MFKSFFPKPGPFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIVGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4870..6027
                     /gene="ampH"
                     /locus_tag="RH033_004093"
     CDS             4870..6027
                     /gene="ampH"
                     /locus_tag="RH033_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HDV2993201.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVIRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6028..6651)
                     /gene="iprA"
                     /locus_tag="RH033_004094"
     CDS             complement(6028..6651)
                     /gene="iprA"
                     /locus_tag="RH033_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HDV2993202.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGSCAGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6737..9685)
                     /gene="ehaB"
                     /locus_tag="RH033_004095"
     CDS             complement(6737..9685)
                     /gene="ehaB"
                     /locus_tag="RH033_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
                     /protein_id="HDV2993203.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMIYSECMTTDCADRADDYYHDRLALTVDNSTIDDNYEHYTYNGTYNN
                     AADTHVVNVFNIGTAITLDQEVDLSISNNSHVAGITLTQGYEWEDIDDNTVSTGVNSS
                     EVFNNTITVKDSTVTSGSWTDEGTTGWFGNTGNASDYNGDGWNADDVALAVIAHPYAD
                     NAMQTTATFDNSTLMGDVFFSSNFDENFFPQGRDSYRDADGDVDTNGWDGTDRLDLTL
                     NNGSKWVGAAMSAHQIGVDTDDDGVNDSYTYGINTEATATLIDIAANSLWPSSTVGVE
                     NSDSEYSEFDHIIGNEVYQSGLFNVTLNTGSQWDTTKTSLIDTLSINSGSTVNVADST
                     LISDSISLTGLSALNINEDGHVATDSLTVDNSTVTISDEVSAGWAVGDAALYANNIKV
                     TNDGILDVGNTAANALQVDTLNLTSTTDTSGNIHAGVFNIESNRFVLDADLTNDRTND
                     TTKSNYGYGLIAMNSDGHLTINGNGDNDNTASIEAGQNEVDNNGDHVAAATGNYKVRI
                     DNATGAGSIADYNGNELIYVNDKNSNATFSAANKADLGAYTYQAEQRGNTVVLQQMEL
                     TDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTI
                     NYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAH
                     FANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVT
                     PYGSVSGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLA
                     YVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDW
                     SANVGVKYTW"
BASE COUNT         2594 a         2473 c         2353 g         2526 t
ORIGIN      
        1 acttataatt aagagaaaaa aactcactgc atggccatac aagccaggaa taaattagct
       61 tcccgttcat catttttgtg atcttgcgca catcattgag ataacgcact ttcttcacca
      121 gtacgttgca cgctttgtca gcagaagatt gcaacattct gtcagaaggc cgcgaatccc
      181 ccgttttgtc gtggtaatgt atgcactttg tgttagacgg gataggtttt taagatggaa
      241 aaactgcggg taggaatcgt ttttggtggt aaatcagcgg aacatgaagt gtctctgcaa
      301 tcggcaaaaa acattgtcga tgccattgat aaaagtcgct tcgacgttgt gctgctgggc
      361 attgataaac aagggcaatg gcacgtcagc gatgccagca attatctgct aaatgcagac
      421 gatcctgccc atattgcgtt gcgcccttcg gcgaccagcc ttgcgcaggt gcctggtaaa
      481 catgaacatc agcttatcga cgcgcaaaac ggtcagccgt tgccgacggt ggatgtcatt
      541 ttcccgattg tccacggtac gctgggcgaa gatggttcct tgcagggaat gctgcgggtc
      601 gccaatttac cgtttgtagg ttctgatgtt ctggcttcag cggcctgtat ggataaagat
      661 gtcaccaaac gtctgctgcg cgatgccggg ctgaacattg cgccatttat taccctgacg
      721 cgcgctaatc gtcacaacat cagttttgcc gaagtggagt ctaaactggg gttaccgctg
      781 tttgtaaaac cggctaatca gggctcttct gttggtgtca gcaaagtaac cagtgaagaa
      841 cagtacgcaa ttgccgtcga tctggcgttc gagttcgacc ataaagtgat cgttgagcaa
      901 gggatcaaag gtcgtgagat cgaatgcgca gttctgggca acgacaatcc gcaagccagc
      961 acctgtggcg agatcgtact caccagcgat ttttatgcct acgacaccaa gtacattgac
     1021 gaagatggcg cgaaagtggt tgttccggca gccattgcgc cagaaatcaa cgataagatc
     1081 cgggcgattg ccgttcaggc ctatcaaacg ttgggatgcg caggcatggc gcgtgtagac
     1141 gtgtttttaa ccccagagaa cgaagtggtg atcaacgaga tcaacaccct gcctggcttc
     1201 accaacatca gtatgtatcc gaagttgtgg caagccagcg gtctgggtta caccgacctg
     1261 atcacgcgtt tgattgagct ggcgctggag cgtcacgctg cagataacgc actgaaaacc
     1321 acaatgtaat attcaaacgc ccggtaatat tcttattggg cgttttcagt ttcttcatcg
     1381 cgacgacgaa taatcaaccc cgccagccag aaactaatca cccacgtcac cagaccgacg
     1441 gcataggtct gccaaccttt tgcttcaaat cccagtaacc ccaccacgcc attaagaatg
     1501 aatataaggc caatggcgaa cgcatagtag tgccagtcac ggcggatttt tacaggcaga
     1561 ttcatggcag ctccgttaac gaaaaacaac atccttgcat attctgtaac aactgtctgt
     1621 ggggtagaca acactttcgc atttgttaat aaatttaact taaggtttcc attttgtgac
     1681 tcacaacgaa gattcacgct tcgggaatat tcccaacatc aatttaccat gattctgata
     1741 ttgacaatct aaatgacctg tcagacttgc cataagccgc tggtatcaca ccagaagaga
     1801 agcagatgac ttccggaggt ttttatggct gatttcaccc tgtcaaaatc gctgtttagc
     1861 ggaaaatatc gcaatgcctc ttcaacgcct ggcaatattg cctatgcgtt gtttgtgctg
     1921 ttttgctttt gggctggggc gcaattgctg aacctgctag tgcatgcgcc cggcgtctat
     1981 gagcgcttaa tgcaggtcca ggaaacaggt cgcccacggg tggaaattgg tttaggtgtc
     2041 ggcaccattt tcgggctgat cccgttttta gttggctgcc tcatttttgc agtagtggcg
     2101 ctatggctgc actggcgaca tcgccgccag taagatttac ttgccagata ggacctaaaa
     2161 cgctttatct ggcatgacat tcctgtttga tcactgtttc atacaacgcg cccgccgttc
     2221 gtctacgcgc ttcgcaaacc aggccgtagt caggttgcgg gtgattttcg ggctttccag
     2281 ttgaatgcca ggcaacattt cgcggggtag tgatttaccc gttttcgctt cggcaagttg
     2341 ataaactttc ttgtacagcg ccgtctcctc aaaagagaag ctgtcccctt tctctaactg
     2401 gcggcgaatt tcactgtcgc tcatccccag ttttcccgcc agtttgcgcg ttgccagttc
     2461 ggttttcccg ggctctttac tgtcgtaacg aattaaatca ccgtccagtg ccagcttcac
     2521 gccgctgacc ttactgaccg cgttctgaaa cgcggcatta cggctggcgt accaaccagc
     2581 gttaaaatca gcaaaacggt atatcggcgc gctatagctg gcgggataat tcagtaaatg
     2641 gtaagtacca aaccacaacc cgccgcgacg actgaagact tcctgacgga ctgtaccgtc
     2701 cattttccac ggataccctt tggtatgctg ttcggcaaag gcaatgctga cctgcatcgg
     2761 cccaccggtg cgcaccggat taagcgaacc aaacaacgtc tgccccattg gcaccatgct
     2821 gatcagatcg tcaaaaatcg cgctcaattg cttttcagta cgcaccgaat ctaaccgttc
     2881 gctatagctt ttgccgtttg gcgatttgat ttttagcgcg gtatgcacca gaaaagcagg
     2941 aatgtgcatc cgttcagcac gacggtcaat ctcttgccag gcgatcttgc ttaagcccgg
     3001 aacagccgga tctgcctgat aatttgactc ctgctgcgcc accgcaagca ccgagcagac
     3061 gttttccagc gtcggtgcca gcccctgact ttcaaacgtg gtggcgagat ctttcgccca
     3121 ggcatcgcgg tctttcacgc tcgcaggcat cttctgccgc acgacgctcg ccacatctat
     3181 cgctttttcg ccctttttca acggttgtgg agcctgacta ctacaccccg ctaaaaccag
     3241 aaccgccaac aatgacagcg aagatagatg attgacgcgc gacattactt ctccttttta
     3301 gctcaaggta tgggttactt cctgaatttt gtcaccatcc agctcatgtt caaagctgcg
     3361 cagacgtttg tagatagaca tcaactcaac cagtgtggtc catgagttaa tcaggtactg
     3421 gaaagcaccg cgaacctgac caaaaacgtt agtaatctgc gtcatcaggc cgagcgtaat
     3481 cgtaccggca acaattgacg gaaacagcaa gaacaaaccg aaaacgttat cgacctgcaa
     3541 atagagaatg cgggcgatgt tgaaatacat atagtgaaaa tagaggcgga aatagttttt
     3601 ccgtacggcg ctaaacagct cgcgtaccgt aggcggcgtc gcgcgcgtgg catcgtcttc
     3661 accataaacc agctctttac ggtaggcagc ctctacacgc tggtttttaa actccagccc
     3721 cggcagtttg atccctacca ctgccagtaa tccggtcccc atcagcgacc agacgatagc
     3781 ggcaatcacc agaccatacg gaatgtgccc gacaatcggc agctccggca catgtgcgga
     3841 gagcgttacc agcaccggca ggaaggcgat caacgtcatg atagcgttga taaagctgac
     3901 gcccatattc tccagcgttg aagcaaaacg catggtgtct tcctgcacac gctgcgcggc
     3961 cccttcgata tgacgcagtt gttgccagtt cgccatgtaa tattcgttca tcgccgtacg
     4021 ccagcggaac acgtagtgac tgacaaagaa gttgttcagc acactgatca ccaccgcgat
     4081 cagcgcaatc cccagaaaga ctcccacttc gcggtaaaat tgttcgatgg tgactttatg
     4141 cggcgaactt agcgccgttt gaatcagatc atagaacggc gcataccagg cgttgacggc
     4201 aaccccgact tccaccaaaa accaggtgac gaagataatc agcgcagtac cgagtattga
     4261 ccagtattgc caacggtgcg ggctatagat aaaccagaac aatgcaaaaa gtcctacgca
     4321 aacaatgtag taagcgtaaa aaatcaggaa atccaacgac cagaaacgcg cggcgctaat
     4381 cgggatctgc ccggaagcgc cggtgatacg cgccacccaa tcccccccac cggcttgcca
     4441 gaagataacg gcaatcaatg cccaaacaaa ggccgagaga aaaaacggtc ccggctttgg
     4501 gaaaaaagac ttaaacatat gcactcctgc taacttctta tcgttttaac aactgctgtg
     4561 tattaaccgc atgaccgcta caaagcgtca gccaaatgaa accgcccatt gtgacccata
     4621 gcaaggcgct tagttcgcca gcctgagcat aagaattgtt tcgacggatt tcacaaacca
     4681 aagattgttt acataagcgg attaacacgc gcaggatcac aaatgcaggc attggtgagc
     4741 aggaaaaaga tcgtgtttac gcgccgttac gtttttaaca ctattattgc actaattcgc
     4801 cctttgcaat ccatcaatga gtagtataaa tacgctcagt taccttcatt caatctatgg
     4861 acaccaccgt tgaaacgtag tctgcttttt tctgccgtgc tgtgtgcggc gtcattgacc
     4921 tccgtccatg cggcgcagcc gatcactgaa ccggagtttg cctctgatat tgtcgatcgt
     4981 tatgccgatc atatttttta cggcagcggt gccacgggga tggcgctggt tgttatcgac
     5041 ggtaaccagc gcgtctttcg cagttatggc gaaacgcgac ctggtaataa cgttcgcccg
     5101 cagctggatt ccgtcatacg tattgcttcc ctcaccaagt taatgaccag tgagatgctg
     5161 gtgaaattgc tcgaccaggg gaccgtgaag ttaaacgatc cgctaagtaa atatgctcca
     5221 ccaggcgcac gtgtaccgac ctacaatggg acgcccatta cgctggtgaa tctggcaacc
     5281 cataccagcg ccctgccccg tgaacagccc ggtggcgcgg cacatcgtcc ggtatttgtc
     5341 tggccaacgc gcgagcaacg ctggaaatac ctttctacgg cgaagctgaa agccgcgcca
     5401 ggcagtcagg cggcgtactc caacctcgca ttcgatctgc tggcagatgc actggcgaat
     5461 gcgtccggca agccttatac ccagctgttt gaagagcaga ttacccgtcc gttggggatg
     5521 aaagacacca cctacacccc ctcaccggat cagtgccgcc gtttaatggt tgctgagcgc
     5581 ggtgccagtc cgtgcaataa cacgctggcg gcaattggca gcggcggcgt ctattccacg
     5641 ccaggcgata tgatgcgctg gatgcagcag tatttgtcat ctgattttta tcagcgcagt
     5701 aaccaggctg atcgcatgca gacgttgatt tatcagcgcg cgcagtttac aaaagtgatt
     5761 ggcatggatg tccccggcaa agcggatgcg cttggtttag gctgggtata tatggccccg
     5821 aaagagggtc gtccagggat tattcagaag acaggtggtg gtggcggttt cattacctat
     5881 atggcgatga tcccgcagaa gaacattggc gcatttgtgg tggtcactcg ttcgccattg
     5941 acgcgcttca aaaatatgag cgatggcatc aatgacctgg tgaccgagct aagcgggaat
     6001 aaaccgttgg ttatccccgc atcctgatta atactctgaa ggcaaacggt tgatagcgac
     6061 cagtttgcct ttattcattt cgatatagcc gcctttacgc aaagcagcga gaacttccgc
     6121 aacgacagaa cgcgatatac gtgtacgttg atggatataa ttcatcacac caatacgtga
     6181 tcgcaattct tcattccagt caatcattga taataacgtc gcgcggattt gttcgtagga
     6241 attatgccca atgagctgaa cgtcgcgtaa ttccagaatt ctattttgcc aggctaacca
     6301 gtaaaaagcg tctcgccaga gttgattttg ttcaataaga gtaatggttt gtttggctgg
     6361 taaatgatat cccgcacagc ttccttctga tattaatttg tatggtatat cgtttttcat
     6421 taaaccatca gccagcccca taatataagg agcctgggta ataccgataa gtacgttttc
     6481 ttctctacgc aaagagataa cgccctctaa aataacgaaa gtatcttcgc tattgacatc
     6541 actggaaaat ataacttgct tttcattatt aaactcgaag cgcgtaccgt atctggacaa
     6601 acatttatcg agcttaccaa attcctgaag aggtttaact acagataaca tttgcgcgtc
     6661 ctttgcagta atgcccgtca aatccttgac gggcattatt tagattaaat tacctatttc
     6721 ttcggagtga agaatattac caggtatatt taacacccac attcgcggac cagtcttgat
     6781 ctacgtcacc accaccgagg tagttagcat cggtataggc gctgaagttc ttggtgaagc
     6841 tgaactgagt acccagacca acacgtaccg cagacccttc agtaccgtta tcgatggaat
     6901 caccgttcac atcgttatcg ttgttagagt cgtcgtagac gtaagccagt ttgaagtacg
     6961 gagtcagagc ctggtcttcg ctgtaggtga aggtataacc tgcatctaca cccagttcat
     7021 aacgcatgct gtcgtaagac tgaccatcaa ctttcatgtc gttgctcagc tggtaatcat
     7081 caccagactg gaacagacca gaaacgctgc cgtaaggagt cacgtaacct gcatcaccca
     7141 gtttgaagtc gtaaccggct ttcaaaccaa agccccaagc gtcggagttg gtgctgccgt
     7201 caacgtaagt accgttgctc atggttgcag acaggtcgtt gttgaagtga gagtagctca
     7261 agctaccgtc aacaaagacg ttgttcgcga agtgagcaga agagtagatg taggcagtct
     7321 ggctgtcttg atccacctga ccagaacggt cattcatatc acctttagcg aagcctgcag
     7381 ccgcaccgac gatccactta gcgttgttac cgtcaatttt ggtatcaaca ccgaccatga
     7441 tgccgttaac atcctgatca tagttgatgg tgccgttgtc gccgttgaag ttaccaccga
     7501 agtagcttac ccatgcgccg ccgttatcag ccagaccatg acgagagttg gtcagacgag
     7561 taccaacggt gtcttgttcc aggttccaga tattggtgtt cgcagacggg atgctcagcg
     7621 ccatgttagc gtagtcggtc agctccatct gttgcagaac aacggtgtta ccacgctgct
     7681 cagcctgata ggtgtatgca cccaggtcag ctttgttagc agcagagaag gtcgcgttgc
     7741 tgtttttgtc gttgacgtag atcagctcgt tgccgttgta gtcagcgata gaaccagcac
     7801 cagtagcgtt gtcgatacga actttgtagt taccggttgc agctgcaaca tggtcaccgt
     7861 tgttatcaac ttcgttctga ccagcttcga tagaagcagt gttgtcgtta tcgccgttac
     7921 cgttaatggt caggtgacca tcagagttca ttgcgattaa gccataaccg tagtttgact
     7981 tggtagtatc gttggtacgg tcgttggtca gatcggcatc gagtacgaag cggttgcttt
     8041 cgatgttgaa cacacctgcg tgaatgttac cactggtatc agtagtgctg gtcaggttca
     8101 gagtatcaac ctgcagagca ttcgccgcag tgttacctac atccaggata ccgtcgttag
     8161 tcactttgat gttgttagca tacagagcag catcacctac agcccagcca gcagaaactt
     8221 catcagaaat ggttacggta ctgttgtcga cagtcagtga atcagttgca acatgaccat
     8281 cttcgttgat gttcagcgca gaaagaccag tcagagagat agagtcagaa atcagcgttg
     8341 aatcagcaac attaacagtt gaaccactgt tgatgctcag agtatcaatc agagaagttt
     8401 tggtggtatc ccactgtgaa ccggtgttca gggtcacatt gaacagaccg ctctggtaaa
     8461 cttcgttacc gatgatgtga tcaaattcgc tgtattcaga gtcgctgttt tctacaccaa
     8521 cagttgatga aggccacagg ctgttagctg cgatgtcgat cagagttgca gtggcttcag
     8581 tattgattcc atatgtatag gagtcattta caccatcgtc atcagtatca acaccaatct
     8641 gatgcgcaga cattgccgca ccgacccact tgctgccatt gttcagggtc agatccaggc
     8701 gatcagtacc atcccaaccg ttagtatcta catcaccgtc agcatcacga tagctatcac
     8761 gaccttgtgg gaagaagttc tcatcaaaat tacttgagaa gaatacatcg cccatcagtg
     8821 tggaattgtc gaatgttgca gtggtttgca tggcgttatc agcatatgga tgtgcaataa
     8881 cagctaatgc aacatcatca gcattccacc catcaccatt gtaatcacta gcattaccag
     8941 tattaccaaa ccaaccagta gtaccttcat cagtccatga accagaggtc acggtagaat
     9001 ctttaacagt gatggtgtta ttaaatactt cgctgctgtt tacaccagtg ctgacagtgt
     9061 tgtcgtcgat atcttcccac tcataaccct gagtcagcgt aatacctgct acatgagagt
     9121 tattagagat ggacagatca acttcctgat ccagagtgat ggcagtacca atgttaaata
     9181 catttacaac atgagtgtca gcggcattat tgtaggtgcc gttatatgtg tagtgttcgt
     9241 agttgtcatc gatcgttgaa ttatcaacgg tcagcgccag acgatcatgg tagtagtcat
     9301 ccgcacgatc agcacaatca gttgtcatgc actcggaata aatcatgccg tgaatagtgc
     9361 tgtttttgat tgtcaggcta ttggcgttgg tattagtaga aataccatcg tccagatagt
     9421 aggtagaaat cacgccgtta acggtagcgt tattaatgac cggatagata tcgccgttgt
     9481 aataactatc agcggcatag ttattccagc ctacataacc atcatagtaa acgtcatcag
     9541 cataggtggc gtcgttgtag tgatggaaag tattgtaagt ttgaccagaa atatcgttgg
     9601 tcgctgcatt agcctgagag gtgatagcca gagtgcaagc caatgctaat tgtgatacta
     9661 caagtttctt tttccaggag tgcatttgtc atccctcctc agggacgtaa taagttgatc
     9721 catcaattat caatgcatag aaaacatatt atgtttcgcg tttacgatta tgcggcgtta
     9781 tagaggaatg gttcaattat tctttcccct aagtccgatt gcggacatat ataaagcacg
     9841 tgaacacaca caaagaaaga aataacaaaa acacaatatt tcaatacgtt aacgaaagcg
     9901 ttaattttaa aacatagacc atgaaaatat aaaacaacca aaacag
//
LOCUS       DAPHCV010000146         9652 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.147, whole genome shotgun sequence.
ACCESSION   DAPHCV010000146 DAPHCV010000000
VERSION     DAPHCV010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.147"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(325..432)
                     /locus_tag="RH033_004096"
     CDS             complement(325..432)
                     /locus_tag="RH033_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDV2993204.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASLIVNWLNKRK"
     gene            908..2179
                     /locus_tag="RH033_004097"
     CDS             908..2179
                     /locus_tag="RH033_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HDV2993205.1"
                     /translation="MQHNTLPKHDQKMPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYVTLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(2209..3213)
                     /gene="dppF"
                     /locus_tag="RH033_004098"
     CDS             complement(2209..3213)
                     /gene="dppF"
                     /locus_tag="RH033_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HDV2993206.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(3210..4193)
                     /gene="dppD"
                     /locus_tag="RH033_004099"
     CDS             complement(3210..4193)
                     /gene="dppD"
                     /locus_tag="RH033_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDV2993207.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(4204..5106)
                     /gene="dppC"
                     /locus_tag="RH033_004100"
     CDS             complement(4204..5106)
                     /gene="dppC"
                     /locus_tag="RH033_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HDV2993208.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(5116..6135)
                     /gene="dppB"
                     /locus_tag="RH033_004101"
     CDS             complement(5116..6135)
                     /gene="dppB"
                     /locus_tag="RH033_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HDV2993209.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRLRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(6443..8050)
                     /gene="dppA"
                     /locus_tag="RH033_004102"
     CDS             complement(6443..8050)
                     /gene="dppA"
                     /locus_tag="RH033_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HDV2993210.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVSPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVAA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(8757..>9652)
                     /locus_tag="RH033_004103"
     CDS             complement(8757..>9652)
                     /locus_tag="RH033_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDV2993211.1"
                     /translation="ARQVLAQSPAPKITVTRACLFLGHSRQAWYQDNTRRRKRQEHHA
                     QVLDFVARVRCRQPRIGARKLHYLLNTQADKRLNIGRDRLFNLLDEYRLLVPVKRAYH
                     KTTNSHHRFYRHPNLLKPGPEQVTALEPEQVWVADITYLPLRNGTAYLSLVTDACSRK
                     IMGYHVGENLQTENVVKAFRQALRLRKTTSPLVHHSDRGLQYCSALYQSVHERNGITC
                     SMTDGYDCYQNALAERINGILKNEFLLSRPADLAQAREMVKESVAIYNHERPHLALKY
                     KTPDDVHQAFYRQKSVNVYQD"
BASE COUNT         2210 a         2617 c         2425 g         2400 t
ORIGIN      
        1 taggttcaag aatggccccc gtgatgttgt caggtgcata cctgcaatat gcgggggttt
       61 tctctctcca gcaaccaatg ccaccaggga ttaagccccc gcaacattgc gcctcaccgg
      121 aacctttccg gcttgccgct gattctacga ctgtttttca gctccgaaat ccgtcacttc
      181 ttcactctgt gctgtgcatt ccgcagaata ttatccgctt gcaaccgttg catttttctt
      241 acgaaaaagg ccgcagagtt ttatccctgc ggcctgatcc gggcgcaaat tgccattacg
      301 gcagcctgac gcccgtctga cacgttactt ccgcttgttc agccagttca cgatcagact
      361 ggcaagaatg ccagctatga ccggagccgc taaatcatgc cagaaggcca tgcccagttc
      421 tgcgagcgtc atatagccgc ctgtgttgta atgacaacgt ttcgcggcta ttcttgagtg
      481 gtctaggttc aagattagcc cccgtgatgt tgtcaggtgc atacctgcaa cgtgcggggg
      541 ttttctctct ccagcaacca atgccaccag ggattaagcc cccgcaacat tgcgcctcac
      601 cggaaccttt ccggcttgcc gctgatttta cgattgcttt ttaccgtcag cacccggcaa
      661 tcttcctcat tgcgtgatgc cttccagctt aatttcttta tttgcattag ttgcagttca
      721 tatccgggct gactccagac tggtagtgaa atgcgcaatt ttgtagaatt gcgcctcctc
      781 gcgacctccc cagtgaatct gttgcctcat tttttacata ctgcgtattc gacttctcca
      841 cctgttgcgc aagagaaact gggtttattc atttttgcga ggccgacttc tttctggaca
      901 ggactttatg cagcacaaca cactaccgaa acacgatcag aaaatgccgt ttacacgcta
      961 cgacttcggc tgggttttat tatgcatagg catggcgatt ggtgccggaa ccgtgctgat
     1021 gccagtacaa attggcttga agggaatttg ggtatttatt accgcagcga tcattgctta
     1081 tcctgccacc tgggtagtgc aggacattta tttaaaaacc ctttctgaaa gcgattcctg
     1141 taatgactac accgatatta tcagtcatta cctggggaag aactggggaa ttttcctcgg
     1201 ggttatctac tttttgatga ttatccacgg gatttttatc tactctctct ccgtggtttt
     1261 cgacagcgcc tcgtacctga aaaccttcgg tttaaccgat gccgatcttt cacaatctct
     1321 actttataaa gtcgccattt tcgccgtact ggtggcaatt gcgtctggtg gtgaacgatt
     1381 actgtttaag atttccgggc caatggtggt ggtcaaagta gggattattg tcgtcttcgg
     1441 ttttgcgatg atcccgcact ggaatttcgc caatataacc gccttcccgc aagcctccgt
     1501 cttcttccgc gacgtcctgc ttactattcc tttttgcttc ttttctgcgg tatttattca
     1561 ggtgctaaac ccgatgaata ttgcctaccg caaacgggaa gcggataaag tactggcaac
     1621 ccggctcgcg ctgcgtaccc accgaattag ttatgtcacg ctcatcgcgg tgatcctgtt
     1681 ttttgccttt tcgtttacct tctcaattag ccacgaagaa gccgtttctg cctttgaaca
     1741 aaatatctcg gcactggcac tggccgcgca ggtgatccct gggcatatca ttcatatcac
     1801 ctctacggtg ctgaatatct ttgccgtact gaccgcattc tttggcattt atctcggttt
     1861 ccacgaggca attaaaggca ttattctcaa tctgttaagc cgaattattg ataccaagaa
     1921 aattaactca cgcatgctga ctctggcgat ctgcgctttt atcgtcatta cgttgacgat
     1981 ttgggtctcg tttcgtgtat cggtgctggt gttctttcag ttgggaagcc cgttatatgg
     2041 cattgtgtcg tgcctcattc catttttcct gatctataaa gtcgcacaac tggaaaaact
     2101 tcgcgggttt aaagcctggc tgattctgct gtacggcatt ttgttatgtt tgtcgccact
     2161 gttgaagttg attgagtaaa ccggagcgca tggccccggt tttgtgagtt aacgctgcgg
     2221 attttcatcc tgatcaacag caaaacaagc taccagttga ccgccgtagt cttttagctg
     2281 cggctgcaac tgggtgcagg ggccgaagcg ccgacgacag cgggcgttga aggcgcaacc
     2341 cggcggtgga ttcagtgggc ttggcagttc accggtgagc ttgatgcgct cgcggcgatc
     2401 gtccgggttc aggcgcggcg tcgcggagag tagcgcctga gtgtacggat gacgcgggtt
     2461 attgaagatt tggtccttcg ttcccttctc cacgcagcgg cccaggtaca tcaccatcac
     2521 ttcatcagca atgtgctcta ccaccgacag gtcgtgcgag ataaagacat aagacagccc
     2581 caactcctgc tgcaaatcca tcatcagatt cagcacctgc gcgcgcactg agacgtccag
     2641 cgcggaaacc ggctcatcgg caatcaccac atccgggtcg agcatcagac ctcgggcaat
     2701 ggcgatacgc tgacgctgac cgccggagaa catatgcgga tagcggtcat aatgttcggt
     2761 tttcaggccg actttcgcca tcatcgacag ggctttttcc cgacgctgtt ctttgcttaa
     2821 gctggtatta atcagcagcg gctcctcaag aatttgcccg acttttttac gtggattcag
     2881 cgaaccgtaa gggttctgga agacaatctg gattttctgc cgacgcagct tctgcgcctg
     2941 cggatcgtgc ttgagcagat cctgcccttg gtaatacagt tcaccaccgg tgggcgtttc
     3001 gatcatcgtc agcaaccgac cgagggtcga tttaccgcag ccagattccc ctactaccgc
     3061 cagcgttttg ccgcgttcaa ggttaaacga aacgccgtcc agcgctttaa ccaaacgttc
     3121 cggcgcgaac atacctttct tcaccggata atgttttttc aggtcgatag cctgcaacag
     3181 cggttgttgc gaggtggcct cttgcgtact catagtgtcg gcctcccggc atcatcaagt
     3241 gggtaatggc atttggactg acgcccgtca gcaagcatat tcagcgccgg ttcttcagcg
     3301 cgacatctgt cagtggcata ggggcagcgc gggttaagca ggcagccgtt cgggcggtca
     3361 tacttgccgg gaacaacacc tggcaacgac gccagacgtt ctttatcctg agcaaattcc
     3421 ggcagcgcac gcagcaatgc ctgagtatac gggtgacgcg gcgcatggaa gatggcgtgc
     3481 gcatcgcccg tttccaccac ctgaccggca tacatcacga tgattttatg tgccgcttcc
     3541 gccaccagcg ccaggtcatg ggtaattaac accagcgcca tgttctcttt ctgctgtagc
     3601 tccagcagta gttcaatgat ttgcgcctga atggtcacgt ccagcgcagt ggttggttca
     3661 tcggcaatca gcagttttgg ccgacaggca atcgccatgg caatcatcac gcgctggctc
     3721 atgccgccgg aaagctgatg cgggtaaaca tccagacgcg aagccggatc gggaataccg
     3781 acctgattca acaagtcaat cgcccgctga cgacgagtgc tcttattacc gccctgatgc
     3841 accttaatcg cttccataat ctggaaaccc acggtgtagc acgggttaag gctggtcatc
     3901 gggtcctgga agatcatcgc cacttcggca cccacaaggt tgcggcgctc tttttccgag
     3961 atacgctgca aatcctgacc gttaaactcc agcttttccg ccattacgcg gcccggataa
     4021 tcaatcagcc ccataatcgc cagcgaactg accgatttac cggagccaga ctcccccaca
     4081 atcccgacca cttcgccctg ttttacgctg tagctgatgc ggtctacggc gcggaacggt
     4141 gcgctttcgt cgccgaaatg caccgataat ttatctacat ttaataacgc catctcgaac
     4201 ctcttactgc ttcagtttgg gatcgagcgc gtcacgcaga ccgtcaccca tcaggttaaa
     4261 tgccagcacc gtcagcagga tcgccagacc cgggaaggtc acgacccacc aggcgctttg
     4321 cgcgaactgc aacacgtcgg agagcatggt gccccactcc ggcgttggcg gctgcgcccc
     4381 catgccgagg aaaccaagag cagccatatc gagaatggcg ttagagaaac cgagcgacgc
     4441 ctgaacaatc agcggcgcaa ggcagttcgg gaagatgtta ataaacatct ggcgcattgc
     4501 cccggcacct gccacgcgag acgcggtaac gtaatcgcgg ttaacttcca ccagcacggc
     4561 ggcgcgtgtt aagcgcacat agtgcggcaa ggcaacgaag gtcagcgcca gcgctgcgtt
     4621 accaatcgac gggccgaaaa tcgccaccag caccagtgcc aacagcagac ttggcagcgc
     4681 cagcatgata tcgaccacgc gcatgatgat gttatcgacc aggccgccga agtaaccggc
     4741 gatcaggccg agaataacgc ccataatcag cgataacaca actaccagac agccaaccag
     4801 cagtgacaat cgcgcaccgt acatcagacg cgacagcaca tcacggccta cgtcatcggt
     4861 gcccagcaag tgcgccatgc tgccgccttc ctgccaggct ggcggggcga gcagtgcatc
     4921 gcggaactgt tccgccgggt tatagggtgc aatccagttg gcaaagatcg cgataaacag
     4981 cacgatgacg acgtaaacca gcccgacgac cgcgcctttg ttgcgtttaa aatagtgcca
     5041 gaactcctgt aacggggtca tcggcaccgg tgcgctaatc actttatttt cagtaacctg
     5101 tgacatgatg tccccttact tcttatgacg aatacgcggg ttcaccacgc cgtacagcag
     5161 atcgaccagc aagttgacga ggataatcat cgtcgccacc agcaatacgc cgccctgcac
     5221 taccggatag tcgcggcgtt gcagtgcgtc aatcaaccag cgccccagac cgggccacga
     5281 gaagatggtt tcggtcagaa tcgcccccgc cagcaacgtt cccacctgca ggccgataac
     5341 ggtcaccacc ggcagcatcg cgttacgcag cgcatggacg ataatcaccc taaggcgggt
     5401 caaccctttg gcgcgcgcgg tgcggatgta atcttcaccc agcacttcca gcatcgagga
     5461 gcgtgtcata cgcacaatga ccgccagcgg gatagtaccc agcacaatgg cgggcaagat
     5521 catatgggcg acggcatcga taaagttacc gtcttcaccc cagatggcgg tgtcgatcag
     5581 cataaaaccg gttaacggat tggaatcatc gaggaacacc atgtcgctca cgcgaccgga
     5641 gacgggcgtc aggttccagt gcaccgaaac cagcatgatc agcatcatgc cccaccagaa
     5701 aattggcatc gagtagccgg tcagcgccag gccaaccgct gtgtgatcga aaatggaacc
     5761 acgtttaacc gcagccagca caccaaccgg aatcccgact gcggtcgcaa aaatcatcgc
     5821 gcagacgcca agttccagcg tggcctgaaa gcgcggcacg aactcttccc ataccggaat
     5881 tcggcttttc atcgaaatgc ctaaatcacc gtgcattacg ccccaaatgt aatggagata
     5941 ctgctgccac atcggtttat ctaagccgag ttcagccagc aattgcgcgt gacgctctgg
     6001 ggagatccca cgttcgcccg ccatgatcat caccggatcg cccgggatca tgtggacaaa
     6061 ggcaaatgtg agaagggtaa taccgataaa cgtggggatg acgagtccca aacgtcggag
     6121 aataaactgc aacataaccc ggattctcta taatgacccg cagcctggaa ctgcgtgtct
     6181 gtattgctca caaatcaatt cccgcccttg tggaacaggg aatgaagtat tgctgccgga
     6241 tggcgctgca cgcagaggca cagcgccacc gggtatgcag attgttgaca aagtgcgctt
     6301 tgtttatgcc ggatgcggcg ttaacgcctt atccggccta caaaatcgtg caaattcaat
     6361 acattgcaga attttcgtag gcctgataag cggagcgcat caggcagttt tgcctttgcc
     6421 atcagtcttg tatggctttt aattattcga tagagacgtt ttcgaagtga tgtttgccta
     6481 atggatcaac cacatagcct ttaacttctt tgcgtaccgg ttcaaatacg gtggagtgag
     6541 cgatgatcag tgccggagcc tgatcgtgca tcaccacctg cgcttgtttg tacagttcaa
     6601 cacgtttgtt atggtcgtcg gtggcacgcg ccggttgaat cagatcttca aacggtttgt
     6661 agcaccattt tgagtagttg gagccttgtt cagaggcggc gcagctgaac agggtggcga
     6721 agaagttatc cggatccccg ttgtcgccag tccagcccat cattaccgtc tgatgctcgc
     6781 catctttcgc acgcttgaga tactcacccc attcgtaggt gacaattttg gcctgcacgc
     6841 ccactttcgc ccagtctgcc tgaatcatct ctgccatgcg gcgagcattc gggttatacg
     6901 gacgttgtac cggcattgcc cacaggtcga tggagaagcc tttttccagg cccgcttctt
     6961 tcagcaaggc tttcgctttt tcgggatcgt aggtgtagtc ctgaacgtcg tcgttatagc
     7021 cccacatggt tggcgggatc aggtttttcg ctgccacgcc cgcgccctga taaaccgctt
     7081 tgatgatcgc gtctttgttc accgcgtagg tcagagcctg gcgaactttc acgtcatcaa
     7141 gtggtttttt ctgcacgtta tatgaaagat agccgacgtt aagccccggc atttccatca
     7201 gattgatgga tttatcctgc ttcatgcgag cgatatctgc cgggttcggg tacggcatca
     7261 cctggcattc attcttctgc aatttcgcgt aacgcacgga agcatcaggg gtaatagaga
     7321 aaaccagcgt atcgatctgc ggtttggtgc cccagtagcc atcaaacgct ttgtagcgga
     7381 tacgggaatc tttttggtac tgctgtaact ggaacggacc ggtgccgatt gggttgaggt
     7441 ccagtttttc cggtgtaccg gctttcatca tcgcgtcagc atattctttt gacagaatag
     7501 aggcgaagtc cattgccagg tcagcgagga acggcgcttc cggtcgagtc agcacaaact
     7561 gaacggtgtt gtcgtccacc tttttcactt cgctgatcag ctccggcaag cccatgcctt
     7621 caaagtattc gtagctaccg ccagaaactt tatggtacgg gttttgcgcg tttttctgac
     7681 gatcgaacga gaacactacg tcatcggcat tcagttcgcg cgtcggtttg aattctttat
     7741 tgtcgtgcca cttcacacct ttacgcagat ggaaggtata ggttttaccg tcttcgctga
     7801 cttcccactt ttcagcgagg cccgggctca cttcagtggt gccgatttta aattcaacca
     7861 gacggttata aagcggtacg gaagaggcgt cataggtggt gccggaggta aacagctgcg
     7921 ggttaaaccc ttccggagat ccttctgagc aataaaccag agttttagcc tgaacacttg
     7981 ctgcgacggt catagccacc aggctgagac caagcttcag catccctgac tttttcaagg
     8041 aaatacgcat tattctgctc caattgtgat gtttgttgtt ttaacccttt gcagtgggtt
     8101 gtcgctgcct gacctttttt gttttttgcc cggtcgggtc aacgttatga ggtggggatg
     8161 ccgtactaat taacatcagt gtaacagtgc ggatcctcca taaaatgccc ctcgtgacct
     8221 acaatctgtc aacagaatgt gaaaacgtca atacagccaa ccgggattta caccaacggt
     8281 gagaatccac aaacaaagat taaaaaaact tcaaacagct atttgcagca gagaaatttg
     8341 tgctacgcca acacaaccag aacaatcagc ttaatattta gcaacatttg gtgattaagt
     8401 tttatgcatt taatgaaaaa aatctgaggt aaaggtggat atctgagcga ttaataccat
     8461 gaacgctaaa acgcacagcg gaaaatgcca gcgccagcca taagtaacgc aaaaaaagat
     8521 ttaagcaaat ataaaaaaag acaatggttt ctgtcacaaa tccgttaatg gatagtgaga
     8581 tatggggcgc aaatttggca gggaaatgtg tggtgtgtag atcggataag gcggtcgcgc
     8641 cgaatccgac aatgggccgg tacatttgcc tgatgcgacg ctggcgcgtc ttatcaggcc
     8701 tacgtcgagt cgaacctccc cgcccccgga ggttctcatc ctcataggct acatgactag
     8761 tcctgatata cgttgacact tttctgtctg taaaacgcct gatgaacgtc atcgggtgtt
     8821 ttgtatttca gagccagatg tggccgctca tggttataaa ttgccacgga ctctttgacc
     8881 atttcccggg cctgcgccag gtctgcagga cgcgagagta aaaactcatt tttcaggata
     8941 ccgtttattc gctcagccag tgcgttctgg tagcagtcat aaccatccgt cattgaacag
     9001 gttattccat ttctttcatg aactgactga tagagcgctg aacagtattg cagccccctg
     9061 tcagaatgat gcaccagggg actcgtcgtt tttctcagcc tcagcgcctg tctgaacgct
     9121 tttaccacat tttctgtctg caggttttcc ccaacatggt agcccataat ttttctggag
     9181 caggcatcgg tgaccagact cagataggcc gtaccattgc gcagtggaag gtaagtaata
     9241 tcggcgaccc agacctgttc tggttcaaga gcggtaacct gttcagggcc tggtttaagc
     9301 aggtttggat gtcggtagaa gcgatgatga ctgttggtag ttttgtgata tgcccgtttt
     9361 actggcacca ggagtcggta ttcatcgagc aggttaaaca ggcggtctcg cccgatattc
     9421 agccttttat cggcctgagt gttcagcaga tagtgcagct tacgcgcacc gattcggggc
     9481 tgacgacatc tgacacgggc aacaaaatca agaacctgag catgatgttc ctgccgtttt
     9541 ctgcgccttg tgttgtcctg ataccatgcc tgtctgctgt gtcccaggaa cagacatgca
     9601 cgcgtcacag ttattttggg ggccggactt tgcgcgagga cttgccgggc cg
//
LOCUS       DAPHCV010000147         9620 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.148, whole genome shotgun sequence.
ACCESSION   DAPHCV010000147 DAPHCV010000000
VERSION     DAPHCV010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.148"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            155..1219
                     /gene="ulaG"
                     /locus_tag="RH033_004104"
     CDS             155..1219
                     /gene="ulaG"
                     /locus_tag="RH033_004104"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDV2993212.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPFMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADILRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1327..2082
                     /gene="ulaR"
                     /locus_tag="RH033_004105"
     CDS             1327..2082
                     /gene="ulaR"
                     /locus_tag="RH033_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HDV2993213.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2079..2828)
                     /gene="yjfP"
                     /locus_tag="RH033_004106"
     CDS             complement(2079..2828)
                     /gene="yjfP"
                     /locus_tag="RH033_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDV2993214.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCAIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLISETAA
                     QQNEFNNIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            3010..3339
                     /gene="bsmA"
                     /locus_tag="RH033_004107"
     CDS             3010..3339
                     /gene="bsmA"
                     /locus_tag="RH033_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HDV2993215.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3488..3763
                     /gene="yjfN"
                     /locus_tag="RH033_004108"
     CDS             3488..3763
                     /gene="yjfN"
                     /locus_tag="RH033_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HDV2993216.1"
                     /translation="MKQFLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3871..5496)
                     /locus_tag="RH033_004109"
     CDS             complement(3871..5496)
                     /locus_tag="RH033_004109"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HDV2993217.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGVRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(5580..6743)
                     /locus_tag="RH033_004110"
     CDS             complement(5580..6743)
                     /locus_tag="RH033_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HDV2993218.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGVIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAEQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFDGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(6746..7384)
                     /locus_tag="RH033_004111"
     CDS             complement(6746..7384)
                     /locus_tag="RH033_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HDV2993219.1"
                     /translation="MARKRKSRNNKNIGHGAISRIGRPHNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKVSTVSRGGYGRSSSARGHWGG"
     gene            complement(7394..7792)
                     /locus_tag="RH033_004112"
     CDS             complement(7394..7792)
                     /locus_tag="RH033_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HDV2993220.1"
                     /translation="MHILDSFLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAAALAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(7810..8469)
                     /locus_tag="RH033_004113"
     CDS             complement(7810..8469)
                     /locus_tag="RH033_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HDV2993221.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSRIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKAMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(8519..9217)
                     /locus_tag="RH033_004114"
     CDS             complement(8519..9217)
                     /locus_tag="RH033_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HDV2993222.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(9236..>9620)
                     /locus_tag="RH033_004115"
     CDS             complement(9236..>9620)
                     /locus_tag="RH033_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HDV2993223.1"
                     /translation="ALATALQTVPEQNIDVTNSENALIIKMNDYGDLQINILFTSRQM
                     IIETFICPVSCISNTDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALSLKSSLE
                     DILLEITSLVDNALDLAEITEEYSH"
BASE COUNT         2512 a         2550 c         2273 g         2285 t
ORIGIN      
        1 cattcaattt aatctttcca cactcaaaat ccacaataac tctcctgcta aaaagtcaaa
       61 aaatggtcat tgccttcctg aaaaaatagt gtcaggatta atcaaaacca atcacatatt
      121 gattcgaatt gaactatcac gaaggtaaat ggcgatgagt aaagtgaaaa gtatcacccg
      181 tgagtcctgg atcctgagca ctttcccgga gtggggtagc tggctgaatg aagaaattga
      241 gcaagagcag gtcgcccctg gcactttcgc aatgtggtgg ctcggctgta ccgggatctg
      301 gttgaaatcg gaaggtggca ccaacgtttg cgttgatttc tggtgcggta ctggcaaaca
      361 aagtcacggt aacccgttca tgaaacaggg tcaccagatg cagcgcatgg ctggcgtgaa
      421 aaaactgcag ccaaacctgc gcaccacccc gtttgttctt gatccgtttg cgattcgcca
      481 gatcgacgcg gtactggcaa ctcacgatca caacgatcat atcgacgtca acgtcgctgc
      541 tgccgtgatg cagaactgtg ctgatgacgt accgtttatc ggaccgaaaa cctgcgtgga
      601 tttatggatt ggctggggag taccgaaaga gcgttgcatc gtggtcaaac caggcgatgt
      661 ggtaaaagtg aaagacattg aaattcatgc ccttgacgcg ttcgatcgta ctgcactgat
      721 caccctacca gcggatcaaa aagcggctgg cgtgctgcca gacggcatgg acgatcgcgc
      781 agtaaactat ctgttcaaaa caccgggcgg ttctctgtat cacagcggcg attcccacta
      841 ctctaactac tatgcaaaac atggcaacga gcatcagatc gacgtggctt taggttcata
      901 cggcgaaaac ccacgcggta ttaccgacaa aatgaccagt gctgatatct tgcgtatggg
      961 cgaagcgctg aatgctaaag tagtgatccc gttccaccac gatatctggt caaacttcca
     1021 ggccgatccg caagagatcc gcgtgctgtg ggagatgaaa aaagatcgcc tgaagtatgg
     1081 cttcaagccg tttatctggc aggtcggcgg caaatttacc tggccgctgg ataaagacaa
     1141 cttcgagtac cactatccgc gtggtttcga tgattgcttc actattgaac cagatctgcc
     1201 gttcaagtcg ttcctgtaat ctgtcgcttg taccggatag cgtggagatg ctcgcgctat
     1261 ccggcatagt atttcctggc tatttcaaat atcatctaaa aaaatcaaat tttatcggaa
     1321 tagctcatga ctgaagcaca aagacatcaa atcctcctgg aaatgctcgc acaattgggc
     1381 tttgtgaccg ttgagaaagt cgttgagcgt ctgggaattt cgcctgccac tgcacgacgc
     1441 gatatcaata aacttgacga aagcggcaaa ctgaaaaaag tgcgcaatgg cgcagaagct
     1501 attacccaac agcgcccgcg ctggacgccg atgaatctgc atcaagcgca gaatcacgat
     1561 gaaaaagtac gtattgctaa agcggcctcg cagctggtta atccgggcga aagcgtagtc
     1621 atcaactgcg gctccaccgc gtttctgctt gggcgggaaa tgtgtggcaa gccagtgcaa
     1681 atcatcacta attatctacc gctggcaaat tacctgatcg atcaggagca tgacagcgtg
     1741 atcattatgg gcggacagta caacaaaagt cagtccatca ctttaagccc gcaaggcagc
     1801 gaaaacagtc tctatgccgg gcactggatg tttaccagcg gcaaagggct gacagcagaa
     1861 gggttgtata aaaccgatat gctgacggca atggcagagc agaagatgct aagcgtggta
     1921 gggaaactgg tggtactggt tgatagcagt aagatcggcg aacgcgcggg aatgcttttt
     1981 agccgtgccg atcaaatcga tatgctgatc accggcaaaa atgctaaccc ggaaatcctg
     2041 caacaactgg aagcgcaagg ggtcagcatt ctgcgtgttt aaagatgctg gcggaaaaat
     2101 gtcacggcag catctaacgc ctcaggcgta atgcggtgac gcacgcctgg ctgccatgaa
     2161 caggttagca gtttatcccg tcccgtctcg cttaaggcct gctgcaaacg tagtgattcg
     2221 tcggcaggca cgacgtcatc gtcaaggcca tgccacagca gtagaggtct gtcaccaagt
     2281 tgttccaggt ggtttgtcgc ttcccactct gccagcggcg cgacaatgtt attgaattca
     2341 ttctgctgtg ctgccgtttc agatatcagc ggtggaaaca gtgaacgggc gagcgatgta
     2401 aaatagcccg atcccatcat gctggcggta catttcaccg tggggtggcg agcggtaatt
     2461 cccagtgccg tcatcgcgcc catcgacgcg ccaccgactg ccagacggtc atcaagcagc
     2521 cagttttctt cggctattgc cgcacgtaaa gtagtgaatt cctgcatact ttgtagcaag
     2581 atttgccaga attgatttaa ccgccgcgct gcgtcgccac taaaacgact accgtgatcg
     2641 ggcgcatccg gcatgatcac ccgcaaacca gcctgcgcca gcgcaacggc aaaatagcta
     2701 tacaccagac tggacgaagt aaagccgtga taaaaaatcg cgcacggtaa cggggtatct
     2761 ttttgcccga caggataagc atgaagaacg ggaatatctg ccagctcgcg tgattctatt
     2821 tcaatcatct gtctctcctt tttgtctggt atgacatgaa gaaatcctta gcacaagttt
     2881 ctgctggcgg tattagttca taatgttgag aagtgggtta cgctttcatc acgttttcat
     2941 tcgaaaatcg cgtaccaggt aacaattcgg gaacattccc ccaaagttaa attaataagg
     3001 cactacacta tggttagcag gaaacgtaat agcgttattt accggtttgc cagtttatta
     3061 ttggtgttga tgttaagtgc ctgtagcgca ctgcaaggta cgccacagcc agcaccgcca
     3121 gtaaccgacc atccgcaaga gattcgccgc gaccagacgc aagggttaca gcgaataggt
     3181 agcgtaagca cgatggttcg gggttctccg gatgacgcat tagcagaaat cagagcgaaa
     3241 gctgtcgctg caaaagctga ttattacgtt gtcgtcatgg tggacgaaac catcgtcaca
     3301 ggacagtggt attcacaagc cattttgtac cgtaaataaa tcagacaact tacatagtca
     3361 gctttacatt gctttgcgtc cctatgcgtt tacctgtgca caataaatta ccggctggaa
     3421 gcctgggcgt ttattaccgc gatggaatgc cctgcaacat gtggggaaac gaaaaatgga
     3481 gctgacgatg aaacaattcc ttgcctcacc ctcgctgcaa ttagtgactt atcctgcgag
     3541 cgccacggca cagtctgcag aattcgccag tgctgactgc gtgacaggcc tgaatgaaat
     3601 aggtcagatc tcagttagca atatttcagg ggatccgcag gacgtggaac gtatcgtggc
     3661 gttaaaggct gatgaacaag gtgcgtcatg gtaccgcatt attaccatgt acgaagacca
     3721 gcagcctgat aactggcgcg tacaggcgat cctgtatgcg tgagtgtcat ctacagcaaa
     3781 tgaacaatgc cggatgcggc gcgagcgcct tgtccggcta acaattttca catatttcat
     3841 tgagttaatc gaaaccagcg tcgtatacac ttacaaacac actcctcccg tcgcccgcag
     3901 caataaatca ttctggatct gctctgacaa ccgtacgccg ccgcgcgtat ctaacatcac
     3961 ctgacaccac gcctgcgcca ttggcggaga ggcatatttc aacatttgcg caccgcagcc
     4021 cagcaggaat agctgatgag taatctctcg ccccagttct tcagcaggct tacgcagctg
     4081 ctgctgtaaa cgacgaaccg cgcgatcaaa atagcgatcc tgccctttca cttccacaaa
     4141 tgcttccgac aataagtcgt atacacccgc ttgcttattg agcacgcgca gtacatccag
     4201 gcacataata ttaccggaac cttcccaaat actgtttacc ggcatctccc ggtaaagccg
     4261 cggcagctcg ctctcctcgc aataaccaat gccacccagc acctccatcg cttcggccac
     4321 aaacggcata cctcgtttgc agatcacaaa tttcgccgca ggcgtaaata aacgcgccca
     4381 cagggcttct ttggcatcgg cacgccggtc ccaggctcgc gcaagacgaa acagcaacgc
     4441 cgtttgccct tcaagctgaa gtgccatgcg acttaagaca tgacgcataa ggggctgttg
     4501 gatcaatgga ttaccaaaaa catggcgttg atgtgcatga taaatcgcta gcgaaaatgc
     4561 acggcgcatc atggcatggc tacccagggc gcaatcaaaa cgcgtcatcc cacccatttt
     4621 caggatcaga cgaactcctt ccccttcctg ccccaacaac cagccaatgg catcctgaaa
     4681 ctccacttcg cagctggcgt tagagcgatt acccagctta tctttcagcc gctcgaggcg
     4741 aatcgcgttg cgttgcccgt caggcaaaaa gcgcggcaca aaaaagcagg acaaaccgcc
     4801 cgtagtctgc gccagcacca gatgcgcatc gctttgcggc accgagaaaa accatttatg
     4861 ccccaccagc cgataagagc catcttccag gcgctctgcg cgggtggtgt tgctcatgac
     4921 atcggaaccg ccctgctttt ccgtcattcc catgccaatc aacaaaccgc gtttttgccc
     4981 acctggcaat aagtgagaat cgtagcgatc gctcaacagc ggcgtggtcc agtcctgaaa
     5041 cggcgcgggt aacatctgta acagcaatgg cgtggcggca aaggtcatcg ttatcggaca
     5101 taacgaccct gcctcaacct gtgcgtgcaa cataaaacgt gccgcgcgcg ccacaaatgc
     5161 gccggagcga gcgtcttctt cccaggcaag attgtgcacc cgattggtac atagcgcctg
     5221 catcagcagg tgccaggcgg ggtgaaaacg cacatcgtcc agacgccgcc cctgggcatc
     5281 gtagcgcaat aattcaggcg gattcacatt cgccagccgc cccagttcaa gggattcagc
     5341 cgtccctaac tgctgaccaa tactggctag aaaatcgcta tcccagccag caccttcacg
     5401 cgttaccgct tcgcagagcg cgccatcaga caggtataag ttgctgttat ttaatggtat
     5461 aggttgatta aaaacggtgt gagtttgcca gtgcactgtg tctccctcca tcaatggcag
     5521 tcaccattag tatggtcact gccatggatt catgacagaa agagattgct aaaacattct
     5581 tatccagcaa tgtaatgcgg aacgaaacgt gaggtgtctt tggtgatcag cgtgttatct
     5641 tcacggatcc ccattccgca cgcttcatca tcgacaatcc agctaccgat gagtgtgtag
     5701 ctatcgccaa atcgcggcag aggttgaaac gcctggtaga tcatcggttc atcggcgtaa
     5761 tcaccatcag cgtggtcaac aacgttattc tgaccgtcga aaatggtgac gtttccgcct
     5821 tcgcgcgagt aaatcggttt acgcacatag ctttcgccag cggcaatctg cggtttttca
     5881 ccatcgaacc aggatgcaag aaggttagga tgaccaggga agaagcgcca aagcagcggc
     5941 attagccctt tattactcaa gatacttttc cataacggct ccacccattg ctcgcgacgc
     6001 ttgcgcagca gcggaccgtt atcgtcacgc atcatccact ccagcggata cagcttaaat
     6061 gcacgctgga tgacattatc atcaagatcg gtcagcacac cgccgacgcc caaaccgaga
     6121 tcttcaatgt agataaaccg cgactcctgc cctgcctgct cggcgcagtc ctgcaagtac
     6181 agcacggtac tcctgtcttc gtcggtgtcc tgacagcagc aaaaataaaa cggttcccga
     6241 ctgtaaagct cgctaaagcg tgaaatcagg cgctcctgaa tggcattgta ctgatcggca
     6301 tcgcgcggaa taacgccgct gcgccgggca tcttccagcc acaaccactg gaaataagcc
     6361 gactcgtaca atgaagttgg cgtatcggcg ttgtactcca gcaacttaac cggtgcatta
     6421 ccacaccagg caaaatccat acggccatac aacgaaggat cgcgtgcgcg ccagctttcg
     6481 gcgatcacat cccagtacaa cggcgggatc gccagttgcg tcaggatctc ttcgtcttta
     6541 accgcgcgat ccaccacctc aaggcacatc tgatgcagtt ccgcagtcgg tttttcgatc
     6601 tgctcttcaa tctggcgcag agtaaaacgg taagctcgac tctcgtccca atagatttca
     6661 ttgtcgatga tatgaaagtc gaaaccgttg tcagcggcga tctggtccag atcccgccgt
     6721 acaggaacgt tgtgtctcag catgattagc ctccccaatg cccacgggcg ctggaagaac
     6781 gaccatagcc gccgcgggaa acggttgaca cttttttggt ggtaaagccg cccgaagagt
     6841 aagactcttt tttgccagaa ccggagcgcc aggagtaatc accagaagtg ttgcgccaga
     6901 cggggcgcga agcaaatgag gatccgccgc tgttatagac gaacggctca tcgcgatctt
     6961 tacgaataac ccggcttaac aaaaatccag aaacgaccgg gatccaactc tgttcaacat
     7021 tgtcgtagta gcaattttcg tacttagact gacaattctg ctgagtcata ttcttcggaa
     7081 catcggcata aaacgccgct ttggcgttgt tccagccacg cgcgcaaatg tctgcattat
     7141 taccgtcatc aatacaatcc tgcacagtcg cgtaaaacgt tccgtcgccg tcgttatcga
     7201 catcgccact atcgccacaa cctttcaata cgaaaaaagc ggcaccgccc attagcgcca
     7261 acgttaagta tttttgtgcg tagcgattgc ggcgcggctc aaaaggatta tgcggtctac
     7321 caatgcgact gattgcccca tgtccgatat ttttgttatt tctggatttg cgttttctgg
     7381 ccatgatcgt ccattaccat gtcatacaag ctgcgttaaa aataccgcca gccagcgcgg
     7441 cggttcccat aaacattcct gctgcggtat tgtgattaat aattttttcg cttaatgcgg
     7501 gcatataaag cctgacgcca gcaaaaacga gtaattgaat caccagagca atcccgcccc
     7561 atgcgaaata gtctggaata ctcaccgcat tgattgccgc actggataag gggatcacgt
     7621 accccaacaa tgttccgctg aacgccagtg ccgccgcggt gttattattt ttgattaact
     7681 gccattcgtt gtgcggtgta attttggagt agataaacag aaaaataatc accatagcca
     7741 cgccaataaa aaaataagcg ctaaaggcaa gaaatgaatc cagtatatgc atcgtaaacg
     7801 tccttgtaat taaccaataa tatgaagtga tgtcagcggg atatcgaccc ccagcgcgcg
     7861 cgaaaataac cactccggct cgccgagatc attaaaagat tcctcaccat ttaacagcag
     7921 atattcgtaa gtatcttcgg tcacttgccg ttgatagccc atggtaaaat tatggacctc
     7981 ccatttggca tggttttgat tttctacttt ttccagcatg tagacgggtt cgatatttcc
     8041 cggttcttcg ctattaaaaa atcgctgcca gcgtttttcc tgccagttta aggtcattgc
     8101 ccccatcgct ttggcattga tcgcctcgcg ccagtgactc tctttactga taccgtagct
     8161 ctcttcatag acaaagagct tgatatcatc aatgtcatca atatcttcgc cgccagtggt
     8221 attgatttgc agaaactcat cgcctgaagt gtagtagcgg aaaatctggc taccgccgcc
     8281 cagatcgatg cggctgacag cggctaccgt aaattcctca cccggcagcg caatcagcaa
     8341 ttcatcttcc agcaaacgaa acgctaacgt gtcgagcgta aagccactat tgagatgaag
     8401 tcccagagga ccgcgagcga tagcaggctt attatccttg ccaaacagac gctggaaaaa
     8461 accagacata ttttatcctt aaaatagttg ccagccttgc agtgaggctg gcaaaaaatt
     8521 actcgccctg ttggcgttgc agtcttgcta atacatcctg ggcgctactt ttattgctac
     8581 cgccaattcc ggcttccgcc agcttttcat caagatcgcg accgtctgcg actttttcca
     8641 actgtgcggc agcatccagg cgagcctgac gttcggcctg acgcgtttgc aggcgtttta
     8701 aggattctgc cgctgtcgaa acgctggaag atgcgccaac ggtagaggtc gttacagcct
     8761 gttgtgcacg ctgcatggct tcagtagctt taacgacttc catttgctgc tcaaactgag
     8821 cgatacgctg cgctgtcgct gtaactgctt tttccacgcc atcacgagag acttccagat
     8881 tcgacaacac ttgctcttca gcggtaatga gattctcaag gcgcgcgatt tcttcagcaa
     8941 cttcgttaat caacgacgga ttaacgttct tgcttaacgc ttccagcgca cgagcttcca
     9001 gactggcttt gcgctcacgt aaatctttca gcttatcgtg acttaatttc acccgcgcca
     9061 gcagatcaac gcgagatttt ccggctttat cgagttcagc tttagcgtca cgaatatgct
     9121 gttccagcat gcgcacgccc tgggtttctt caatggattc ttccgcctgg gagataaacg
     9181 atttccccag cgtaaataaa ctttttaaaa tccccataaa tacccgtcct tgtaattagt
     9241 gtgaatattc ttctgtaatt tcagccagat ccaatgcgtt atctaccagc gaggttatct
     9301 caagcaggat atcttcaaga gaagatttaa gcgataacgc accgaaaaca atgtaatact
     9361 cttcctgttg cacgctggag atccctaccg atgacaatgg catcatcttc tgatttctta
     9421 ataagaaggt attaaattca tctgtattgc tgatacaact taccggacaa ataaaggttt
     9481 cgataatcat ttggcgggaa gtaaatagaa tattgatttg caaatcgcca taatcattca
     9541 ttttaataat taatgcgttc tcgctatttg ttacatcaat attttgttca ggtacagttt
     9601 gcagcgccgt cgccagcgcc
//
LOCUS       DAPHCV010000148         9416 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.149, whole genome shotgun sequence.
ACCESSION   DAPHCV010000148 DAPHCV010000000
VERSION     DAPHCV010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.149"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..77)
                     /locus_tag="RH033_004116"
     CDS             complement(<1..77)
                     /locus_tag="RH033_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV2993224.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVV"
     gene            complement(83..781)
                     /locus_tag="RH033_004117"
     CDS             complement(83..781)
                     /locus_tag="RH033_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDV2993225.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDMQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            complement(781..1110)
                     /locus_tag="RH033_004118"
     CDS             complement(781..1110)
                     /locus_tag="RH033_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV2993226.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            complement(1107..3668)
                     /locus_tag="RH033_004119"
     CDS             complement(1107..3668)
                     /locus_tag="RH033_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDV2993227.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSMNRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAINLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQS
                     GLTADRMLVLSRAGQVAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWSLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQSQQDTNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLERHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQQADKFA
                     QQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            complement(3661..4095)
                     /locus_tag="RH033_004120"
     CDS             complement(3661..4095)
                     /locus_tag="RH033_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HDV2993228.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPEMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            complement(4077..4499)
                     /locus_tag="RH033_004121"
     CDS             complement(4077..4499)
                     /locus_tag="RH033_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HDV2993229.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLDLMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPQKTKLPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAVPVSAGKCSTVS"
     gene            complement(4515..5255)
                     /locus_tag="RH033_004122"
     CDS             complement(4515..5255)
                     /locus_tag="RH033_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV2993230.1"
                     /translation="MPVPNPAIPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPDGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            complement(5263..5658)
                     /locus_tag="RH033_004123"
     CDS             complement(5263..5658)
                     /locus_tag="RH033_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV2993231.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            complement(5655..6233)
                     /locus_tag="RH033_004124"
     CDS             complement(5655..6233)
                     /locus_tag="RH033_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV2993232.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERAWLKRATVKNPQARIKVNRGDLPVIKLGNARIVLSRRRRRKKGQ
                     RSALKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            complement(6245..6598)
                     /locus_tag="RH033_004125"
     CDS             complement(6245..6598)
                     /locus_tag="RH033_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HDV2993233.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            complement(6610..7005)
                     /locus_tag="RH033_004126"
     CDS             complement(6610..7005)
                     /locus_tag="RH033_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HDV2993234.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVTDTADLVTVVALVTLHTDALHATRDEA
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(7047..7835)
                     /locus_tag="RH033_004127"
                     /pseudo
     CDS             complement(7047..7835)
                     /locus_tag="RH033_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
     gene            8190..>9416
                     /locus_tag="RH033_004128"
     CDS             8190..>9416
                     /locus_tag="RH033_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993235.1"
                     /translation="MKGTEISSHLVQPTPLDSTEVTLLQTALSTVRHDVVDCPYISEA
                     IRVLPVKGYRSAITAYWNAVVDDLRQKIIHRSLDLFNKECKQKKEIKTYEDFQDYVSD
                     FDLIDGAFKIGVIDREGQKLLQQARETRNLFGGHPQSSDPDLIKVVNLINDCNKYVLS
                     KEFPPAIIDISTYLSEMDSATFNKNSLAVKQAFSDLPAIYISELSSRFFGTYCSSNIS
                     SELRNNIEFCAPILWTLLSKDDRRKVGQQFDKYLVSGNNNKIEKAQQFILLVDGLMYI
                     SPASRKVIIEPLINKLTESLDNWSEEEKIVTKLLPFSSFVPEDNIVEFVSAITKTYVG
                     HRGFSYQYGRKNFYSNGAAPLILEMFEHFDINMVSAFVDVIKNDSTLRHRIQEDGQFS
                     RLQKLGELLLNKSTCTP"
BASE COUNT         2107 a         2808 c         2228 g         2273 t
ORIGIN      
        1 cttaccacga agccgcacga ctccgctggc gcacatcgcc gggcgtgcgc cagaatcgct
       61 gattctgtct gtgtcatggg atttactgcg aaagtttgtt aatggaaagg aagccgccaa
      121 agttgccgac gttattgcga aacttacagc cactcaggca tttgctgcat ttatccttcg
      181 tgatatcgga cgttggctgg tcatattcat ccgcgaccgc cggaccgtga taaccgcact
      241 catcgccgcg ataggtccag gtgcaggtgt tggccagcat gatacgtccc ggaaaaacag
      301 cgccatccgt ttccgtcggc gtggacagta caaaggaggc actgaccgcg ctcagttcgc
      361 tgcactgctc gatgcgccag cggctgatca cctcctgctc cggatcggcg tcgctgtttc
      421 cgttgacgaa gttcaccgca tccagaaaac gggcgtaaac cttacgccgg accaccgttc
      481 cgccgaccag actctgcata tcttccgcca tcccggtgac cataccgtac aggttagaaa
      541 ccgtcagcgt ggggcgtgta ctggtgcctt tgccattcag ttcaaaaccg ctcccctgaa
      601 tgggatacgg ctgatactgt cgcccctgcc aggtgaccgg ctcacctttt tcgttctgct
      661 cattacagaa aaaataacgt tctccaccga cctctgtcag gtcgatttcc cagagcacca
      721 cgctggccga ctgctccgca cgggtgcatt cattcagtgt ttcctgccgg atatcctgca
      781 tcagttcacc acctgttcaa actctgcgct gaactcaaca cgcagcatac tgacccgcga
      841 cgaccatttt gcgcaggtca cctttatctg ccgccactca taaggcggcg tccacagaaa
      901 ggctttccag cccccgtgct cttccagaaa cgactccagt accgtggcct cctcacgggg
      961 gacagaaagc gtcacgctgt acgttttcag gttggcattc agcccggcag gcgctcgctg
     1021 ggaatagcca tcaccaaagc gcacctttct tacagaaggg gccgaagcca catccatacc
     1081 gggtttcact ttccagcgga aggttttcat cgtccacctc cggagaacag gccaccatca
     1141 cgcatctgtg tctgaatttc atcacgggca cccttgcggg ccatgtcata cactgccttc
     1201 atcatctgtg gacctggcag accattcgta ccgtcgttct gaatcaccac gtgattgttc
     1261 tgattaaaat taatgccttc agcccgccgc atctgcgccg gacttccggc accgcccaca
     1321 taaccacctt ccgcatagcc ccgcatcagg cggtacaggt tgccgacacc aatccggctg
     1381 gttgcctcct tcgtgaagac aaattcacca cggtgaacaa tccccgctgg ctcatatttg
     1441 ccgccggttc ccgtaaatcc cccggtcgca aaatggaatt tcgccgcagc tgcctgaatg
     1501 gctgtaccgc ctgacgcgga tgcgccgcca ccaacagccc cgccaatggc gctgccgata
     1561 ctcccgacaa tccccaccat tgcctgctta agcagaattt ctgtcatcat ggacagcacg
     1621 gaacgggtga agctgcgcca gttctgctca ctgccggtca gcatcgccgc catattctgt
     1681 gcaataccat caaaggtctg cgtggcagca cttttaacct gcgacatact gtccgtggcg
     1741 ctctcttccc actcactcca gccggacttc aggcctgcca tccagctccc gcgaagctgg
     1801 tcttcagccg cccaggtctt tttctgctct gacatgacgt tattcagcgc cagcggatta
     1861 tcgccatact gttccttcag gcgctgttcc gtggcttccc gttctgcctg ccggtcagtc
     1921 agcccccggc ttttcgcatc aatggcggcc cgttttgccc gttgctgctg tgcgaattta
     1981 tccgcctgct gcgccagcgc gttcaggcgc tcctgatacg taaccttgtc gccaagtgca
     2041 gccagctggc gtttgtactc cagcgtctca tctttatgcg ccagcaggga tttctcctgt
     2101 gcagacagct ggcgacgttg cgccgcctcc tccagtaccg cgaactgact ctccgccttc
     2161 cacaaatccc ggcgctgctg gctgattttc tcatttgctc cggcatgcct ctccagcgtc
     2221 cggagttcag cctgaagcgt cagcagggca gcatgagcac tgtcttcctg acgatcgccc
     2281 gcagacacct tcacgccgga ctgtttcggc tttttcagcg tcgcttcata gtcctttttc
     2341 gccgccgcca tcagcgtgtt gtaatctgcc tgcagaattt tcccgtcctt cagtgccttg
     2401 ttcagttctt cctgacgggc ggtatatttc tccagcggcg tctgcagccg ttcgtaagcc
     2461 ttctgcgcct cttcggtata tttcagccgt gacgcttcag tatcgctctg ctgctgcgca
     2521 tttgtgtcct gttgactctg ctgttcagcc ttctttctcg cggcttcaag cgcaagacgg
     2581 gccttttcac gatcatccca gtaacgcgcc cgcgcttcat cgttaacaaa ataatcatcc
     2641 ttgcgcagac tccagatgtc gtccgctttc ttaaacgcag cctctgcctt aatcagcatc
     2701 tcctgcgcgg tatcaggacg accaatatcc agcaccgcat cccacatgga tttgaatgcc
     2761 cgcgctgtcc tgtctgccca ggtctccagc gtacccatgt tctctttcag gcggcgggtc
     2821 tggtcatcaa accctttcgt cgcggcctcg ttcgccgcct gcaatgcccc ggcttcatcg
     2881 ccggaacgct gcaactgagc aacatacgca atctgctccg ccgtcacgtt atggaactgg
     2941 cgtgccatcg ccgtcaaccc cgacgtcggg tctgtggtca gcttcccgaa ggcttcagcg
     3001 accttgtcca cctccacacc ggatgcagag gagaaacgcg ccacactctg gctgatggac
     3061 gcaatctgag cctcaccgct tactcccgcc ttaaccagtg cgctgagtga ctcgctggtc
     3121 tggttaaacg tcagccctgc cacctgcccg gctctggaca ggaccagcat gcgatctgcc
     3181 gtcagacccg actgattgcc ggaaaggacc agcgttttgt tgaaatcgga cagggttgag
     3241 ttgccctgat accaggcata cgccagcgca ccggtcgcca ccgccagcga ggtggccccc
     3301 accatcggca ggttgatcgc accggcaagc cccctgaaca tggggatcat cccgccgaag
     3361 gagtccttaa cctgcccccc ctgttgcagc aggatcagcc acggactttg cccgcctgca
     3421 agctgcgtgg ccacgtcggt gaactgtgca ggcagcatac gcatggcggc tttatactgt
     3481 ccgacggaaa tccccgcttt ctgtgcagcc agcgcctgcc ggttcattga ctgttcaacg
     3541 actgccgctg tttttttcgc atcactttcc gtaccggaaa aatgacgcct gactctggcc
     3601 atctgctcgt caaatctggc cgcatccaga cttaaatcaa cgaccagatc gcctaccggt
     3661 tcagccatac cggactcctc ctgcgatccc ttctgatact gtcatcagca ttacgtcatc
     3721 ctccgtcatg tccgccacat ccggggaagc ggggataact tcattcccgt ccgggccaaa
     3781 acgaacgcct ccggcaagcc ctgccgcttt ctgcatcagc acatcatctt caggctcttc
     3841 gtcagcctcg cgccggttca gcagactgaa atccagcgga tgcatctccg gatcgctgaa
     3901 aaacaggctg agcacggtgt acgtcagccc ggaaaagtgc atatccagca gaacatcatg
     3961 aaaataatgg gtactgtaaa agcggtgcca gtcggcatac tccgtggatg acatcccggc
     4021 aagcatggcg cgccagtcgg gtcgccccat ctcacgcgcc agtttcaggg caaaactcag
     4081 ctcaccgtcg aacactttcc cgcagaaaca ggcactgcag gcccggcgtc ctctgcctgt
     4141 tcaggagcat cattcaccac aaactcatac ataccggaca gccggtacac cacgttttca
     4201 gcatgagaaa ttgcctctgt gggccaggtg gtaagcactt cctgctcaat ctgtttaacg
     4261 gcttcattca tggaaggcag ctttgtcttc tgcggatggt tatgccacag ggacatcgct
     4321 accacaaaag caccggttct gatggcgtct tccacagtaa acttccggtt gctgtctgac
     4381 tccgcctgtt ctgcctgccg tttcatcagg tcgagatgct caatacgctg cagggctgac
     4441 agttcagaaa gcgtgacggt cacaccgtta tgttcaaatg attcggtttt caggaacatc
     4501 gctgactctc cggattaact ggcggtgacg ttgatttctg caaccgcagc aaactcacca
     4561 ttaccagata cgaccggaat gttgaccttg cctgcagcaa cacctttcac ggtgatggtc
     4621 ataccactga ccgacacggt ggcttttgtt ttatccgcag acaccgcacg gaagctcttg
     4681 tcggttgcgc catccggctg gaatgccacg gtcagcgtgg tgctctgccc tttcactacg
     4741 gaagcactgg cgggtgtcac cgtcatgccg gtcgtcgccg tcaccgtgct gcgatcttct
     4801 gccatcgacg gacggcccac attggtgacc ttcaccgtgc gggtaatcac ttccttcgcc
     4861 gtcaccgcct taccgatact gctgacccag ccacggaaca catcgaccgt gccgttcggg
     4921 aagcggattt tataggcacg ggtatcacct tcattaaacc acgccagcag cgcctgctgc
     4981 ccctgctctc cgggcatcca cgccagcgtg aagctggtat ctccggcaga tttctgcccc
     5041 tgcccggtcg cagtccagtc tgcatcttca tcatcgagat agctgtcgtc ataggactca
     5101 gcggtcagtt cgccgggcgt caggtcttta acttttgcca gacgcgacca gtcaacgtct
     5161 gaaagcgggt tcgcataagg gtcaccgctc cccttataaa cccacagggt ggttccggca
     5221 cctttcaccg gtattgctgg atttggtaca ggcatatcgt cctcacattt cataggtaat
     5281 gacatacgtc agatcggcag aactccacaa gcccgcatca tcgtcgcgcc ggtagtcata
     5341 gccactggcc accatactgg tgatcaaatc tgacagtgcc gggatatcgc tcatcaccgg
     5401 ataaatccgg gactccatcc acgcatccag ctctgaatcc ggcacctgag caggcaggaa
     5461 aacttcaata tgcagctccg cctgccaggt atcgctgtcc agctcttcgc ccgtgtattc
     5521 agcgccggtg agataaacgg caattgccgg aaaatcttcc tcatcaaaaa cagcagggcg
     5581 accatcaaaa aacgtcgccc cggtgtcatg cttctccagt gcatccagta cggctgcacg
     5641 gagttcagta tgtttcatcg ctttattacc atcctcagtt gatgctgcag cgcatagccc
     5701 agctctttcg gaagacgctc acgccgtatc cgctcaatat tctgtttaaa cgccgtggtc
     5761 agcggcaccg ccatcgggat tttcaccaca tcaatggggt aacggttttt cccggccaca
     5821 cgctgcatga catgccagcg gccatttttc agttgctgaa taaacgcgcc gggaatacga
     5881 cggtttccca ccacaagcac gctgccgcca cctttcaggg ctgaacgctg ccccttttta
     5941 cgacgcctgc gtcgggacag gacaatccgc gcgttaccca gctttattac gggcaaatcc
     6001 ccccggttaa ccttgattct ggcctgcgga tttttgaccg tggccctttt cagccaggcc
     6061 ctttccttta ccagtttccg gcgtaccttt gtctcacggg caacctgtga cgccgactgc
     6121 gatatcgcgg atgaagcaac gcggttaatg gccattgcgg cggcaccggg caccgccgtt
     6181 ctgctgatac ggctgaggtt ttcaacggcc tgctcaagac cttttatggc catacatccc
     6241 cctttcagcg gcgacggtta acggcaggcg gtacgccccg cccaagccag agatgacagc
     6301 ttccgccatc atccggcgaa atccggtcta tccagaagtt ttcctcaccg atggtcagcg
     6361 tgtcgccgcg ccgcagctgc cgcacatcat cagtccggac aaacagggac gggctggagc
     6421 cttcaacgcg tacgccctgt ccggcatagc tgatattttc agggtcatca aaaacaccac
     6481 gtattactgc gccggactgc tcaccggatg tcatggtggc tgacgttccc atgtacccgc
     6541 gtatcgtttc atcggcgcgg gcaatggcag catcgaacag gttatcgaaa tcagccacag
     6601 cgcctcccgt tattgcattc tggccaggcc gcgctctgtc atttcagctg ccacaccggc
     6661 agagacacgg aacgccgttc ccggcagcac aaatgccaca gcctcatccc gcgtggcgtg
     6721 aagtgcatca gtatgcagcg tcaccagtgc cacaaccgtg accagatcag ccgtatcagt
     6781 cacggtatcc ggctgcgctg ataccacctc attttcatgt ccggtcagcg cattttccgg
     6841 gctgacagat gtgtcctgac cggcagcgtc atccgtgtca tcaagctcct cttccagctc
     6901 tgccacacgg agcgccagtt cttctttcgt ccccgtcagg ctgacatcac ggttcagttg
     6961 ttcacccagc gagcggagac gggcaatcag ttcatctttc gtcatggact cctccacaga
     7021 gaaacaatgg ccccgaaggg ccatgattac gccagttgta cggacacgaa ctcatcaggg
     7081 tcagccagca gcatcagcgg tgctgactga atcatggtga actcacgcgc cggatcgccg
     7141 gtggtcaccc agtttttcgg gtaacgggca gaggcgttaa tgccttcgcg ctgtgcgtcc
     7201 gcatcctgaa tgcagccata ggtgcgcaga ccgcgtgcat gagtgttacc cagcaccatc
     7261 gtgttgtccg gcaggaagtt ctttttgacg ccgttttcca cgtactgtcc ggaatacacg
     7321 acgatggcca catcgccata catcccctta taggacaccg ctttacccag gtctttcacc
     7381 gctgtctcca gctcggaatg agagccgcga cgggtatcca gcttctcctt gacggcgttg
     7441 aaggaacgga acagcgccca gcctttcgga tcaaacacga tgatattcac cacgccgctg
     7501 gcgttcagcg cgtaggcttc gatatcgtcg gtcgggtcat acgtggactt gtcacgcttg
     7561 ctccactccg tgccgccgga ctgcgtgatg ttgttcgccg cactgcggcc catatccacc
     7621 tcaaccggat cgaaggcttc accggtcatg gtgtatttgc ccttgagcac ggcagaaact
     7681 gcctgcatct cttcgacctg agcaatggcc agctcttcgt cacgcatgtt ctgcaggatg
     7741 atgcgacggc ggcggtaagc cgggtccgcc agattctgcg gatcttcatc cggcaggcga
     7801 cgcagggtca tctgcggatt cacttcatgc ttgggttata tgagtcaata tcgaaaggat
     7861 atgattgaat tttcaatata aaattgattg ttgtagcttt cgaaagaagg ttctgcaata
     7921 cattgtgcaa tacaaagtac gctgtcttaa ccttttgctc ctcggtgagg aactagaagc
     7981 agttttgctg tgaactctca cttaacttct tagccctaaa catctgaatt ggaaaaaata
     8041 aaaataatta ataacaatag gttatagaga actgccttat ctcaataagg taacatcaat
     8101 ttttccactt aaaactggca agaacgaact tgatatcttt ttcaaattca atagagtaga
     8161 aaaatggaaa agaataggaa aatcctgaaa tgaagggtac agagataagt tctcatttag
     8221 tgcagccaac tccactagac tctacagaag ttacattgtt acaaactgcg ctctcaactg
     8281 tgaggcatga tgtagttgac tgcccatata tttcagaagc aataagagtt cttcctgtaa
     8341 aaggttatag aagtgccatc actgcttact ggaatgctgt agttgatgat ttacgtcaaa
     8401 agataattca tcgtagccta gatctattca acaaagagtg taaacaaaaa aaagaaatta
     8461 aaacgtatga agattttcag gattatgtga gcgactttga tttaatagat ggagcattta
     8521 aaataggagt tatcgatcga gaaggtcaga aactattaca acaagctaga gaaaccagga
     8581 atctatttgg tggacatcca caaagtagcg accctgattt aatcaaagta gtgaacctga
     8641 ttaatgattg taataaatac gttctttcta aagagtttcc accagccatc atagatatat
     8701 ctacatatct ttcagaaatg gattcggcaa catttaataa aaatagccta gcagttaagc
     8761 aagccttttc agatctacct gcaatttata tcagtgaatt atcctctcgt ttttttggca
     8821 catactgctc atccaatata tcaagtgaac tcagaaacaa cattgaattt tgcgcaccta
     8881 ttttatggac attactttca aaagacgacc gacgtaaagt cggccaacaa tttgacaagt
     8941 atttagtttc aggaaacaac aataagatag agaaggcaca acaattcata ctattggtag
     9001 atggactgat gtatattagc cctgcatccc gaaaagttat tattgagcct ctcattaata
     9061 aactcaccga gtctttagat aattggtcag aagaggaaaa aatcgtaacc aaattattac
     9121 cattctcgag tttcgtccct gaagataata ttgttgaatt tgtttcagcc attacaaaaa
     9181 cttatgttgg ccatcgtggt ttcagctatc agtatggaag gaagaatttt tattcaaatg
     9241 gggctgcacc tttaatattg gaaatgtttg aacactttga tattaacatg gtttctgcat
     9301 ttgttgatgt tattaaaaat gatagtacac ttcgtcacag aatccaagag gatgggcaat
     9361 tctctcgatt acagaaactt ggtgaattac tgttaaacaa gagcacatgc actcct
//
LOCUS       DAPHCV010000149         9347 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.150, whole genome shotgun sequence.
ACCESSION   DAPHCV010000149 DAPHCV010000000
VERSION     DAPHCV010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.150"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..278)
                     /locus_tag="RH033_004129"
     CDS             complement(<1..278)
                     /locus_tag="RH033_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993236.1"
                     /translation="MHILSVSKKEVGRAGDRLVSQFGTGEKYKEEDVQILHEWRMLHL
                     YPLSKIQFYMEREAISLNKNALLSSRIKRMPSIVTKLSRFPDMKLNKM"
     gene            complement(812..1213)
                     /locus_tag="RH033_004130"
     CDS             complement(812..1213)
                     /locus_tag="RH033_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HDV2993237.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRRSGRETLRQLEVKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSAVDPDLIWSLPDG
                     EIRRYDRRQNIICRECRKSEVMQRILAFYQG"
     gene            complement(1254..2219)
                     /locus_tag="RH033_004131"
     CDS             complement(1254..2219)
                     /locus_tag="RH033_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993238.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLLRCEKLNKSKRDMTNFYTINY
                     GSELLDGGKLNESIGSKCAAPSGQNDTMEEVKMKRSIGSKRPNVIGSKWPDDLTENTT
                     EITTENKNTFRPEASQPDPQTTEQDFLTRNPDAVVFSVKKRQWGSREDLACAQWIWGR
                     IVNLYEQAASDDGEIMRPKEPNWTAWANDVRTMRMLDGRSHRQICEMFGRVQRDPFWV
                     KNIMSPSKLREKWDELVIRLGRSPVQRCVNHISEPDTEIPPGFRG"
     gene            complement(2200..2721)
                     /locus_tag="RH033_004132"
     CDS             complement(2200..2721)
                     /locus_tag="RH033_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993239.1"
                     /translation="MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNID
                     VQRVTYEGGAVDQRALSVNRVKIFERWKAIDTRDKREKFTALVPAIMEAIRISDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPAVVDRETDELIEKAIAFKLAYRQQY
                     QQKAGWNYESSFC"
     gene            complement(2705..2932)
                     /locus_tag="RH033_004133"
     CDS             complement(2705..2932)
                     /locus_tag="RH033_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV2993240.1"
                     /translation="MLKVDAITFFGSKTKLANAAGVRLASVAAWGILVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKTKRAGRLNNENHS"
     gene            3013..3420
                     /locus_tag="RH033_004134"
     CDS             3013..3420
                     /locus_tag="RH033_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV2993241.1"
                     /translation="MDTRTLGQRVLARRKELRLTQREAARLAGVAHVTISQWERDETQ
                     PVGKRLFALADALKCSPTWLMFGDEDKAPVPAQELHVETELTPNHKELIELFDALPSS
                     EQEALLSEMRARVENFNKLFEEMLKARKNKSIK"
     gene            3589..3741
                     /locus_tag="RH033_004135"
     CDS             3589..3741
                     /locus_tag="RH033_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HDV2993242.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARCW
                     LARNTD"
     gene            3741..4118
                     /locus_tag="RH033_004136"
     CDS             3741..4118
                     /locus_tag="RH033_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993243.1"
                     /translation="MRLTMEFKDLPSDVQKTAAHTLHSVLREIGKDIASEPAKDLARK
                     IKTAFVELYNVGTDSETVETKTVSSPIFSLEPGVLSGEICTEISSELLPVIREAICRR
                     GLDGSYDHDVLQVLRTMVTSLGI"
     gene            complement(4087..4374)
                     /locus_tag="RH033_004137"
     CDS             complement(4087..4374)
                     /locus_tag="RH033_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993244.1"
                     /translation="MRFRVVCLLLTTSGEVVSFLFRITKKEIYMTKEEFVSYIFDKTV
                     EMYAATYGSCNPLNKPEGKDDFDKIYRFLEDRYIKRLEDAGIKSPVKSPLS"
     gene            4790..4978
                     /gene="dicB"
                     /locus_tag="RH033_004138"
                     /gene_synonym="ftsT"
     CDS             4790..4978
                     /gene="dicB"
                     /locus_tag="RH033_004138"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HDV2993245.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            4975..5166
                     /gene="ydfD"
                     /locus_tag="RH033_004139"
     CDS             4975..5166
                     /gene="ydfD"
                     /locus_tag="RH033_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein YdfD"
                     /protein_id="HDV2993246.1"
                     /translation="MNSAFALVLTVFLVSGEPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVIHQDNIEIPAGL"
     gene            5260..7731
                     /locus_tag="RH033_004140"
     CDS             5260..7731
                     /locus_tag="RH033_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HDV2993247.1"
                     /translation="MSKVFICAAIPDELATREEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KKEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPIALNKYICEY
                     LTTKGVFENEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVEREGPFYF
                     LFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     PEPIKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQTRDSV
                     NQNQHESERNYQKAEQNSTNALQNEPETKQPEPVAQQEVEKVCTACGQTGGGNCPDCG
                     AVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTGGNQHHNSDNETGETADHS
                     IKVNGHHEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSSEFFVQVWG
                     NGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNA
                     LDDARYQAKYVSVIWQKLIPSQADS"
     gene            7804..8055
                     /locus_tag="RH033_004141"
     CDS             7804..8055
                     /locus_tag="RH033_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HDV2993248.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDSMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            8090..>9347
                     /locus_tag="RH033_004142"
     CDS             8090..>9347
                     /locus_tag="RH033_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDV2993249.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTAKNRRVAGEL
                     RSSVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLPDNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKQKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLSEFAPTMP"
BASE COUNT         2698 a         2179 c         2102 g         2368 t
ORIGIN      
        1 tgcatcttat ttaacttcat atcaggaaat ctagaaagct tagtaacaat ggaaggcatc
       61 ctcttaattc tagaggatag taacgcattt ttgtttaagg aaatagcctc tctttccata
      121 taaaattgta ttttacttaa aggatataaa tgcaacatcc tccactcatg aagaatttgt
      181 acatcttcct ctttatattt ttcacctgta ccaaactgag aaactaatct atccccagct
      241 cttccaactt ccttttttga aacagataaa atgtgcatag taagaggcct tataataaaa
      301 ttatgctagt ttaggaatta caacttacaa agacccgaca gcggacaagg ttaacaaacc
      361 agcgccaatt aagagagcta gtccgtgcca gtatggcatc agggtaactg aaatgaattg
      421 gatgcaccag acgtgcgaca aggagcctgc ttgaaaaagg gaattaaagc tgttttttaa
      481 ccaattggct gatcgctact cctcaacttg cggatttatg acgttttagt gcccgaaact
      541 gaatgtaatc attcgttgga gtggctcgaa aaaatccacg aaaatgctca catcagacta
      601 gaagtaccgt acatgagggc atttttgaat tccatttcgc gggatggagg tgaccagctc
      661 ccgtttttaa ctcacagttt cgttagtaac gtccgctgtt ggcacaaagc ggacaaccac
      721 gccagatcta ccctgtgcca tgaaaaatga gaatgtcaat tcactcctga actaatgctt
      781 tttaatctag taacgtctaa aatatctaac attatccctg ataaaatgcc agtatgcgct
      841 gcataacttc actcttccgg cactcgcggc agattatgtt ctgacgcctg tcgtagcgac
      901 gtatttctcc gtcaggtaat gaccagataa ggtccggatc aaccgcagat ggtttcttca
      961 gctttgccct tgagagcttt ttacgggcat tttgccagtc cttacgcgcc tgttcagacg
     1021 ggaataaccc gtaaccagag ttgtatacat cgccgctggc aaccagctct cttgcgagaa
     1081 cgctcatcag atatcttgtc gcacctgtct tgacttccag ttgccgtaac gtctcacgcc
     1141 cactccggcg tacgagttca acaacctgcc ctttaatttt ttcccgctct tcttgtgtaa
     1201 aaacttttgc cacaagtccc ccttaaaatt atctcatgac ctgaaatcaa cacttatccc
     1261 ctgaaaccag gcggaatttc tgtatccggt tcagaaatat gattcacaca acgctgtaca
     1321 ggtgaacgtc ccaggcggat gaccagttcg tcccattttt cgcggagttt tgacgggctc
     1381 atgatgtttt ttacccagaa tggatcccgc tgtacccgac caaacatttc gcaaatttgt
     1441 ctgtggcttc tgccatccag catccgcatt gtgcgcacgt cattggccca ggctgtccag
     1501 ttaggctctt ttggtcgcat gatctctcca tcgtcgctgg cagcctgttc gtaaaggttc
     1561 acgatccgcc cccaaatcca ctgcgcacac gccagatcct ccctgctacc ccactggcgt
     1621 tttttcacac taaacacaac cgcgtcgggg ttccgggtta aaaaatcctg ttcagtcgtc
     1681 tgcgggtccg gttgcgaagc ttccggacga aaagtgtttt tattctctgt agtaatctct
     1741 gttgtattct ctgtaagatc atcaggccat tttgacccga tgacattggg tcgttttgaa
     1801 ccaatggagc gtttcatttt gacctcttcc atcgtgtcat tttgacctga tggagcggcg
     1861 cattttgaac cgatggattc gttcaatttg ccaccatcta aaagctcgct cccatagttg
     1921 atcgtgtaga aattggtcat atcgcgcttt gatttattga gcttttcaca acgcaaaagc
     1981 cccagcgttt tcagacttgc aaacgcgcgc tttaacgttg actctgacca gaatgggaac
     2041 tgttccagcc attgttccgt tgtgttataa atccagcgaa caccatcaca ttccataccg
     2101 gagttggtat ctctcaacca gtagtgcagt tgttgcaaaa caatggcttc gtttaagccg
     2161 attttcattg ccagctgcgt gtttataacc agtgggcgtt cagcaaaaag aagactcata
     2221 attccatcca gctttttgtt ggtattgctg tcgatacgca agcttgaaag caattgcttt
     2281 ttctataagt tcgtcagttt cacgatctac aacggcagga tctgcaaaaa gcagtccgga
     2341 ttccaccaca tcgccatatt ctttatttaa cccggcgatc atgtacgtaa tactttttcc
     2401 atcactgatc tcacgataca acctgaaatc actaatccgg atagcctcca taattgcagg
     2461 cactagcgct gtgaactttt cacgcttatc cctggtgtcg atagccttcc agcgttcgaa
     2521 tatcttcact cgattaacgc taagcgctcg ctgatcaacc gcgccacctt catatgtgac
     2581 acgctgaaca tcgatgttcg ggcgctcttt caaagcccag aatgcttcag tgattaatat
     2641 cgtcgcctgc tcctgtgtca ttcctggtcg acatatccag gcatccagag cctcacgagc
     2701 ctgttcagga gtgattttca ttgttcaacc gccccgcccg cttcgtctta cgatattcgt
     2761 cataaacttt gggatcatac tgaagctccc cgccagatgc ctcctgtaga cgcatcgcgc
     2821 gaccttcagg aaccagtatc ccccaagcag caacacttgc cagtctcact cctgcggcat
     2881 tggcaagctt tgttttgctg ccaaaaaaag taattgcgtc aactttaagc atcaaagccc
     2941 cctcttgtta gacttttcta acattattgt gcgcgggata cctaagtcaa gaaaaattag
     3001 aattacctaa ctatggatac aagaacccta ggccagcgag ttctggcgcg acgaaaagaa
     3061 ttacgcttaa cacaacgaga agctgcgcgc ctcgctggag ttgctcacgt cacaatttca
     3121 caatgggaaa gagacgaaac ccagccagtc ggaaaacgat tgtttgcttt agcggatgct
     3181 ctgaagtgct cacctacatg gctaatgttt ggtgacgaag acaaggcacc agtgcctgca
     3241 caagaacttc atgtggaaac agagctaact cccaaccaca aagaattgat cgaattattc
     3301 gatgctcttc catcttccga gcaggaagcc ttgctgtctg aaatgcgcgc aagagtagaa
     3361 aacttcaaca aactcttcga agaaatgctt aaagcgcgta aaaataaatc aataaaataa
     3421 cattcttttc aagtgattag ttgcgcccac tctttttgtt agatcaatct aacaaaaaac
     3481 acttgcctct catgttaggt tattctaaat tactttccat caagacaccg cacggtgttc
     3541 tcagcaaaca gttccgcgac cccggcgtta aggggaaatg aggtcagcat ggatactatc
     3601 gatcttggca acagcgaatc tctggtatgt ggcgtgttcc ccaaccagga cggtacgttc
     3661 accgcgatga catataccaa aagcaaaacg tttaaaaccg aatctggcgc gcgttgctgg
     3721 ttagccagaa acactgactg atgaggctga cgatggaatt taaagattta ccgtctgatg
     3781 tacagaaaac agcagctcat acattgcatt ctgtgctgcg agaaatcggg aaagatattg
     3841 caagcgagcc agcaaaagat ctggcccgga aaatcaagac cgctttcgtt gagctttata
     3901 atgttggcac tgactctgaa actgtcgaga ccaagaccgt aagttcacca atattctcac
     3961 ttgagccagg cgtattatca ggtgaaatat gcaccgagat ttccagcgaa ctgcttccgg
     4021 taatacgaga agcaatttgc cgtcgcggtt tggatggaag ttacgatcat gacgtcctgc
     4081 aagttctcag gacaatggtg acttcactgg ggatttgatc cctgcgtcct ctaacctttt
     4141 gatatagcgg tcctccaaga agcggtaaat tttgtcgaaa tcatctttcc cctctggttt
     4201 attcagagga ttacaggacc cgtaagtagc ggcatacatt tcaaccgttt tatcaaaaat
     4261 ataagagaca aattcttctt tagtcatata gatttccttc ttggttattc ggaataagaa
     4321 ggataccacc tcgcctgacg tggttaaaag caggcacaca acacgaaagc gcacggcgaa
     4381 gttcgtctca ctgtacggtg tcgttaaatt taattcgacc gtgcgcttcc ggttgtggca
     4441 acccgcgaaa tggcgcggcg gtaagtatgg cagggttatt ccttccccgt tgaggacacc
     4501 gagttgtcag gttgaccata cgcttaagtg acaaccccgc tgcaacgccc tctgttatca
     4561 attttctggt gacgtttggc ggtatcagtt ttactccgtg actgctctgc cgccgttttt
     4621 aaagtgaatt ttgtgatgcg gtgaatgcgg ctaagcgcac gcggaacagt taaaaccaaa
     4681 aacagtgtta tgggtggatt ctctgtatcc ggcgttaatt gttaactggt taacgtcacc
     4741 tggaggcacc aggcactgca tcacaaaatt cattgttgag gacgcgataa tgaaaacgtt
     4801 attaccaaac gttaatacgt ctgaaggttg ttttgaaatt ggtgtcacta tcagtaaccc
     4861 tgtatttact gaagatgcca ttaacaagag aaaacaagaa cgggagctat taaataaaat
     4921 atgcattgtt tcaatgctgg ctcgtttacg tctgatgcca aaaggatgtg cacaatgaat
     4981 tcagcatttg cgcttgttct gacagttttt cttgtttccg gagagccagt tgatattgca
     5041 gtcagtgttc acaggacaat gcaggagtgt atgacagcag caaccgaaca gaaaattccc
     5101 ggtaactgtt acccggtcga taaagttatt caccaggata atatcgaaat cccggcaggt
     5161 ctttaaaaca gttccgtaat aaatatccgg tttcattctt atatgccagc aatggcaggg
     5221 atttgttcat ccttaaatct gtcatgaggt taaaacaaaa tgagtaaagt ctttatttgc
     5281 gccgctattc ctgacgaact ggcaacaagg gaagaaggcg ctgtggctgt agccacagcc
     5341 attgaagctg gcgacgaacg ccgtgctcga gcaaaatttc actggcagtt cctggaacat
     5401 tatccggctg ctcaggactg cgcttataaa tttattgtct gcgaggataa acctggcata
     5461 ccccgccctg ccctcgattc atgggatgct gaatatatgc aggaaaaccg ctgggatgag
     5521 gagtctgctt cttttgtccc ggttgagact gaatccgatc cgatgaacgt cacttttgac
     5581 aagctggccc ctgaagtaca gaacgctgtc atggttaagt tcgacacatg tgaaaacatc
     5641 accgttgata tggttattag cgcacaggaa ttgttgcagg aagacatggc aacattcgac
     5701 ggacatatcg ttgaagcgtt gatgaaaatg ccagaagtta acgccatgta tccggagctt
     5761 aagttgcacg ccattgggtg ggttaagcat aaatgtattc ctggtgctaa atggcccgaa
     5821 attcaggcag agatgcgcat ctggaaaaaa cgtcgcgaag gtgaacgcaa ggaaaccgga
     5881 aaatacacgt ctgttgttga tctcgcccgc gccagagcca atcaacagta cactgaaaat
     5941 tcaacaggaa aaatcagccc ggtcattgct gccattcatc gcgaatacaa gcagacatgg
     6001 aaaacactgg atgacgaact ggcctacgct ctctggcctg gtgatgtgga tgccggaaac
     6061 attgacggca gcatccatcg ctgggcaaaa aaagaagtta tcgacaacga ccgcgaagac
     6121 tggaagcgta tctcggcatc aatgcgcaaa cagcctgatg cccttcgcta cgaccgccag
     6181 actatttttg gccttgtccg tgagcgtccg atcgacattc acaaagatcc catagcactg
     6241 aacaaatata tctgcgaata cctgacgaca aagggcgtgt ttgagaatga agaaacagac
     6301 ctgggcactg ttgatgttct ccagtcatca gaaacacaaa ctgatgcagt ggaaactgag
     6361 gtatctgata tcccaaaaaa tgaaaccgcg ccggaagctg aaccatctgt agagcgtgag
     6421 gggccgttct atttcctctt cgcagataag gacggagaaa aatacggtcg cgcaaacaaa
     6481 ctttctggtc tggataaggc actggctgct ggcgccactg aaatcacaaa agaagaatat
     6541 tttgcccgaa aaaatggcac atacacgggc ttaccgcaaa atgtagatac cgctgaagat
     6601 tcagaacaac cagagccgat aaaagttacc gctgacgaag taaacaaaat tatgcaggca
     6661 gccaatatca gccagcctga cgccgataag ttgcttgctg catcacgtgg tgaatttgtt
     6721 gaagggatta gtgacccgaa tgatccgaaa tgggttaagg ggatccagac ccgcgattct
     6781 gtgaaccaga accagcatga atcggaacgg aactaccaaa aagcggaaca aaacagcaca
     6841 aatgcgttac aaaacgagcc agaaacgaaa cagcctgaac cagtggcgca acaggaagtg
     6901 gaaaaagtct gcaccgcctg cggtcagacc ggcggcggca actgccctga ttgtggcgcg
     6961 gtgatgggcg acgcaacata ccaagaaaca ttcgatgaag agtatcaggt tgaagttcag
     7021 gaagatgatc cggaggaaat ggaaggcgct gaacatccac acaaggagaa cactggcggc
     7081 aatcagcatc acaatagcga taatgaaact ggcgagacgg cagatcactc aattaaggtg
     7141 aacggtcatc acgaaatcac atccaccagc aggacgtgtg accatctaat gatcgacctt
     7201 gaaaccatgg gaaaaaatcc tgatgccccg atcatctcaa taggtgcaat atttttcgat
     7261 ccgcaaaccg gagatatggg accggaattt agtaagacta tcgatctgga aactgctggc
     7321 ggagtcattg atcgggacac cattaaatgg tggcttaagc aatcacgcga agcgcaatct
     7381 gccattatga ccgatgaaat cccgttagat gatgcactgt tacaattgcg ggaatttatc
     7441 gacgaaaact ccagtgaatt ttttgttcag gtttggggaa atggagccaa cttcgacaac
     7501 acgattttgc gccgttcata cgaacggcag gggatcccct gcccgtggcg ttactacaac
     7561 gatcgcgatg tacgcacaat cgttgagctg gggaaagcca tagacttcga tgccagaacg
     7621 gctattccat tcgaaggtga gcgccataat gcacttgatg acgcccgtta ccaggcaaaa
     7681 tacgtttcag ttatctggca aaaactgatc ccgagtcagg ctgattctta atgttcaact
     7741 gtcgccggtt gtgactggta ttctgcaacc ggcgctcgtc tgatgtaaga gataaagaaa
     7801 tcgatgagcg aagtaatcat gattgtctct cccggcaaat gggtatccga agagcagtta
     7861 attgcgctga aaggaataaa aaaaggtacg ttaaaaaagg cccgggaaaa atcgtttatg
     7921 gaaggaaggg aatataagca tgtcgctcat gacagtatgc catgggataa cagtccatgc
     7981 ttttacaacc tggaagaaat tgatcgctgg attgagcgcc aggcatcagc gagaccaaga
     8041 cgtcatctta cttgactaaa agccacacta actaatgaga gaagttgaaa tgaaatatcc
     8101 gacaggcgtg gaaaaccatg gagggaaatt acgtatctgg tttgtttata aaggcgtaag
     8161 agtcagggaa aatctggggg ttcctgacac agcaaaaaac aggcgcgttg caggtgaact
     8221 acgctcctct gtttgttacg caataaaaac tggtgttttc gactatgcaa aacagtttcc
     8281 ctcctcacgc aatctggaaa aatttggtga ggcccgacaa gatttaacca taaaagaact
     8341 ggctgaaaaa tttctggcac tgaaagaaac tgaagtcgcc aaaacatcac tcaacacata
     8401 ccgtgccgtc atcaaaaata tcctgagcat aatcggtgaa aaaaatcttg cctcatcgat
     8461 taataaagaa aaattactgg aggttcgtaa agagctactg actggatacc agatccccaa
     8521 aagtaactat attgttacac aaccagggag atcggctgta actgtaaata attacatgac
     8581 aaatcttaac gccgtgttcc agtttggtgt tgataacggt tacctgccag ataatccgtt
     8641 taaggggatc tcgccattaa aggaatcaag aaccattccg gatcctcttt cgcgggagga
     8701 atttatccgt cttatcgatg cgtgcagaaa tcagcaagca aaaaatttat ggtgtgtttc
     8761 tgtttatact ggcgttcgcc ctggtgagct gtgtgcactt ggatgggagg acatagatct
     8821 gaaaaatgga acaatgatga tcaggagaaa tttagcaaaa gaccgtttca cggtaccaaa
     8881 aacacaggcg ggaaccaatc gggtcattca tcttattaag ccagcaatcg acgctctccg
     8941 gagtcagatg acattaacga gactgagcaa agagcatatc attgatgttc acctcagaga
     9001 gtatggcaga acagaaaaac aaaaatgcac ctttgttttt caacctgaag tgtcagcgag
     9061 agtaaaaaat tatggtgacc attttaccgt tgactcaata aggcagatgt gggacgcagc
     9121 gataaaacgt gccggactcc gccatcgaaa atcatatcag tcgagacata cttatgcctg
     9181 ctggtcgctg acagctggtg ctaacccggc atttatagca aaccagatgg gccatgcaga
     9241 tgcgcaaatg gtatttcagg tatacggaaa atggatgtct gaaaacaata atgcacaggt
     9301 agctttgtta aatacacagt taagcgagtt tgccccaacc atgcccc
//
LOCUS       DAPHCV010000150         9307 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.151, whole genome shotgun sequence.
ACCESSION   DAPHCV010000150 DAPHCV010000000
VERSION     DAPHCV010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.151"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..220
                     /gene="rzpD"
                     /locus_tag="RH033_004143"
     CDS             <1..220
                     /gene="rzpD"
                     /locus_tag="RH033_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prophage endopeptidase RzpD"
                     /protein_id="HDV2993250.1"
                     /translation="LRDDVAAGRRRLHIKAVCQSVREATTASGVDNAASPRLADTAER
                     DYFTLRERLITMQKQLEGTQKYINEQCR"
     gene            complement(252..545)
                     /gene="iss"
                     /locus_tag="RH033_004144"
     CDS             complement(252..545)
                     /gene="iss"
                     /locus_tag="RH033_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HDV2993251.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGFLGFITLGIYTPLEARVYCSQ"
     gene            complement(907..1101)
                     /locus_tag="RH033_004145"
     CDS             complement(907..1101)
                     /locus_tag="RH033_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HDV2993252.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDE"
     gene            1490..2035
                     /locus_tag="RH033_004146"
     CDS             1490..2035
                     /locus_tag="RH033_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HDV2993253.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            2010..3935
                     /locus_tag="RH033_004147"
     CDS             2010..3935
                     /locus_tag="RH033_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HDV2993254.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDAPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            3932..4138
                     /locus_tag="RH033_004148"
     CDS             3932..4138
                     /locus_tag="RH033_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HDV2993255.1"
                     /translation="MTRQEELAAARAALHDLMTGKQVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            4135..5736
                     /locus_tag="RH033_004149"
     CDS             4135..5736
                     /locus_tag="RH033_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDV2993256.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSKEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAHFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            5717..7036
                     /locus_tag="RH033_004150"
     CDS             5717..7036
                     /locus_tag="RH033_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HDV2993257.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSVDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            7046..7378
                     /locus_tag="RH033_004151"
     CDS             7046..7378
                     /locus_tag="RH033_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HDV2993258.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVDADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            7434..7679
                     /locus_tag="RH033_004152"
                     /pseudo
     CDS             7434..7679
                     /locus_tag="RH033_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
     gene            7842..9077
                     /locus_tag="RH033_004153"
     CDS             7842..9077
                     /locus_tag="RH033_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDV2993259.1"
                     /translation="MAGENKLSDKALKGYLGKPREKQITIADGKGLSIRVSTKGAVSF
                     VFFYRLAGGRAAPVWLTLGKYPDMSLKQAREKRDECRGWLADKRDPRIQIKIQAEERL
                     KPVTVEDALNYWYENYCKVRRKTHAVTLGRFRKHIFPYIGHLPVNDTHLYEWLDCFDR
                     IKRNAPVMAAYVFSDTKLALRFCRVRQYATCDALKDLRMSDVGQIAGKRDRVLDEAEL
                     GQLWKAIFVEPDLKLMSEYTRKMFVLCTVFGCRMSEARLSEWSEWDLESWVWTVPKDH
                     SKTGVEIVRPVPEILRQWVTDVHEETKHTGYVLGSLRIRESVSKIGGKIGKRLGHEKQ
                     WSLHDLRRTLSTHLSDLGVEFYVVEQLLGHALPGVAGVYNRSKFMAKKLDALELWTTY
                     LNSIAGADSKVTILKQKVG"
     gene            9079..9306
                     /locus_tag="RH033_004154"
     CDS             9079..9306
                     /locus_tag="RH033_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HDV2993260.1"
                     /translation="MKKMAIVDKKGLEYIPNIDRMIREKECRELTTLANSTRWKLEKE
                     GKFPKRIKIGSTAVAYRLSEVQAWIRGEWVV"
BASE COUNT         2243 a         2235 c         2669 g         2160 t
ORIGIN      
        1 tctgcgtgat gatgttgccg ctggtcgtcg tcggttgcac atcaaagcag tctgtcagtc
       61 agtgcgtgaa gccaccaccg cctccggcgt ggataatgca gcctcccccc gactggcaga
      121 caccgctgaa cgggattatt tcaccctcag agagaggctg attactatgc aaaaacaact
      181 ggaaggaacc cagaagtata ttaatgagca gtgcagatag agctgcccat atctatgggc
      241 aactcatgca attattgtga gcaatacaca cgcgcttcca gcggagtata aatgcctaaa
      301 gtaataaaac cgagaaatcc atttacgaat gtttgctggg tttctgtttt aacaacattt
      361 tctgcgccgc cacaaatttt ggctgcatca acagttttct cctgtccaat tcccgaaacg
      421 aagaaatgat gggtgatggt ttcctttggt gttactgctg tcggtttgtt tccaacagta
      481 aacgtctgtt gagcacatcc tgtaataagc attgccagag cggcagaaaa caacattttt
      541 ttcatcttat tatcctgcat tgttaaaaac ggcagaatcc tatgtgacaa caattaaacg
      601 atagttaaat ggattgatga aaattaaaac tatataggtg gatgctcagc ctattggagg
      661 aggggggggc actcagaatc ctgtggaatg aaataaaccg ctctttctgt ccattaccct
      721 tttagctgcg ctgtatcgtc gccgtattcc cgcattaacc atgaccgtag cccgacgggg
      781 aattccttct gcgtgagtgt gcgggaataa tcaaaaacga tgcacaccgg gttttactgt
      841 gctgacagac gcagggttac cctcatagtc gcttttccgg tgcgatggtg gaagaaaccg
      901 ggatgtttat tcatcatcac tctggattga tgtatatgct ctcttttctg acgttagtct
      961 ccgacggcag gcttcaatga cccaggctga gaaattcccg gacccttttt gctcaagagc
     1021 gatgttaatt tgttcaatca tttggttagg aaagcggatg ttgcgggttg ttgttctgcg
     1081 ggttctgttc ttcgttgaca tgaggttgcc ccgtattcag tgtcgctgat ttgtattgtc
     1141 tgaagttgtt tttacgttaa gttgatgcag atcaattaat acgatacctg cgtcataatt
     1201 gattatttga cgtggtttga tggcgtagat gcacgttgtg acatatagat gataattatt
     1261 atcattttgc gggtcctttc cggcgatccg acaggttacg gggcggcgac ctcgcgggtt
     1321 ttcgctattt atgaaaattt tccggtttaa ggcgtttccg ttcttcttcg tcgtaactta
     1381 atgtttttat ttaaaatacc ccctgaaaag aaaggaaacg acaggtgctg aaaacgagct
     1441 tttgggcctc tgtcgtttcc tttctctgtt tttggccgtg gaatgaacaa tggaagtcaa
     1501 caaaaagcag ctggctgaca ttttcggtgc gagtatccgt accattcaga actggcagga
     1561 acagggaatg cccgttctgc gaggcggtgg caagggtaat gaggtgcttt atgactctgc
     1621 cgccgtcata aaatggtatg ccgaaaggga tgctgaaatt gagaacgaaa agctgcgccg
     1681 ggaagttgaa gaactgcggc aggccagcga gacagatctc cagccaggga ctattgagta
     1741 cgaacgccat cgacttacgc gtgcgcaggc cgacgcacag gagctgaaaa atgccagaga
     1801 ctccgctgaa gtggtggaaa ccgcattctg tactttcgtg ctgtcgcgga tcgcaggtga
     1861 aattgccagt attctcgacg ggatccccct gtcggtgcag cggcgttttc cggaactgga
     1921 aaaccgacat gttgatttcc tgaaacggga tatcatcaaa gccatgaaca aagcagccgc
     1981 gctggatgaa ctgataccgg ggttgctgag tgaatatatc gaacagtcag gttaacaggc
     2041 tgcggcattt tgtccgcgcc gggcttcgct cactgttcag gccggagcca cagaccgccg
     2101 ttgaatgggc ggatgctaat tactatctcc cgaaagaatc cgcataccag gaagggcgct
     2161 gggaaacact gccctttcag cgggccatca tgaatgcgat gggcagcgac tacatccgtg
     2221 aggtgaatgt ggtgaagtct gcccgtgtcg gttattccaa aatgctgctg ggtgtttatg
     2281 cctactttat agagcataag cagcgcaaca cacttatctg gttgccgacg gatggtgatg
     2341 ccgagaactt tatgaaaacc cacgttgagc cgaccatccg cgatattccg tcgctgctgg
     2401 cgctggctcc gtggtatggc aaaaagcacc gggataacac gctcactatg aagcgttttt
     2461 ccaatggtcg tggcttctgg tgcctgggcg gtaaagcggc aaaaaactac cgtgaaaagt
     2521 cggtggatgt ggcgggttat gatgaacttg ctgcctttga tgaggatatt gaacaggaag
     2581 gctctccgac gttccttggc gacaaacgta ttgaaggctc ggtctggcca aagtccatcc
     2641 gtggctccac gcccaaagtg agaggcacct gccagattga gcgtgcagcc agtgaatccc
     2701 cgcattttat gcgttttcat gttgcctgcc cgcactgcgg ggaggagcag tatcttaaat
     2761 ttggcgacaa agagacgccg tttggcctca aatggacgcc ggatgccccc tccagcgtgt
     2821 tttatctctg cgagcataat gcctgcgtca tccgccagca ggagctggac ttcactgatg
     2881 cccgttatat ctgcgaaaag accgggatct ggacccgtga tggcattctc tggttttcgt
     2941 catccggtga agagattgag ccgccggaca gcgtgacctt tcacatctgg acggcgtaca
     3001 gcccgttcac cacctgggtg cagattgtca aagactggat gaaaacgaaa ggggatacgg
     3061 gaaaacgtaa aaccttcgta aacaccacgc tcggtgagac gtgggaggcg aaaattggcg
     3121 aacgtccgga tgctgaagtg atggcagagc ggaaagagca ttattcagcg cccgttcctg
     3181 accgtgtggc ttacctgacc gccggtatcg actcccagct ggaccgctac gaaatgcgtg
     3241 tatggggatg ggggccgggt gaggaaagct ggctgattga ccggcagatt attatgggtc
     3301 gccacgacga tgaacagacg ctgctgcgtg tggatgaggc catcaataaa acctataccc
     3361 gccggaatgg tgcagaaatg tcggtatccc gtatctgctg ggatactggc gggattgacc
     3421 cgaccattgt gtatgaacgc tcgaaaaaac atgggctgtt ccgggtgatc cccattaaag
     3481 gggcatccgt ctacggaaag ccggtggcca gcatgccacg taagcgaaac aaaaacgggg
     3541 tttaccttac cgaaatcggt acggataccg cgaaagagca gatttataac cgcttcacac
     3601 tgacgccgga aggggatgaa ccgcttcccg gtgccgttca cttcccgaat aacccggata
     3661 tttttgatct gaccgaagcg cagcagctga ctgctgaaga gcaggtcgaa aaatgggtgg
     3721 atggcaggaa aaaaatactg tgggacagca aaaagcgacg caatgaggcg ctcgactgct
     3781 tcgtttatgc gctggcggcg ctgcgcatca gtatttcccg ctggcagctg gatctcagtg
     3841 cactgctggc gagcctgcag gaagaggatg gtgcagcaac caataagaaa acactggcag
     3901 attacgcccg tgccttatcc ggagaggatg aatgacgcga caggaagaac ttgccgctgc
     3961 ccgtgcggca ctgcatgacc tgatgacagg taaacaggtg gcaacggtac agaaagacgg
     4021 acggcgagtg gagtttacgg ccacgtccgt gtctgacctg aaaaaataca ttgcggagct
     4081 ggaggtgcag accggcatga cacagcgacg caggggacct gcaggatttt atgtatgaaa
     4141 acgcccacca ttcccaccct tctgggaccg gacggcatga catcgctgcg tgaatatgcc
     4201 ggttatcacg gcggtggcag cggatttggt gggcagttgc gggcgtggaa cccaccgggt
     4261 gaaagtgtgg atgcagccct gctgcccaac tttacccgtg gcaatgcccg cgcagacgat
     4321 ctggtacgca ataacggcta tgctgccaac gccatccagc tgcatcagga tcatatcgtc
     4381 gggtcttttt tccggctcag tcatcgccca agctggcgct atctgggcat cggggaggaa
     4441 gacgcccgtg ccttttcccg cgaggttgaa gcggcatgga aagagtttgc cgaggatgac
     4501 tgctgctgca ttgacgttga gcgaaaacgc acgtttacca tgatgattcg ggaaggtgtg
     4561 gccatgcacg cctttaacgg tgaactgttc gttcaggcca cctgggatac cagttcgtcg
     4621 cggcttttcc ggacacagtt ccggatggtc agcccgaagc gcatcagcaa cccgaacaat
     4681 accggcgaca gccggaactg ccgtgccggt gtgcagatta atgacagcgg tgcggcgctg
     4741 ggatattacg tcagcgagga cggctatcct ggctggatgc cgcagaaatg gacatggata
     4801 ccccgtgagt tacccggcgg gcgcgcctcg ttcattcacg tttttgaacc cgtggaggac
     4861 gggcagaccc gcggtgcaaa tgtgttttac agcgtaatgg agcagatgaa gatgctcgac
     4921 acgctgcaga acacgcagct gcagagcgcc attgtgaagg cgatgtatgc cgccaccatt
     4981 gaaagtgagc tggatacgca gtcagcgatg gattttattc tgggcgcgaa cagtaaggag
     5041 cagcgggaaa ggctgacggg ctggattggt gaaattgccg cgtattacgc cgcagcaccg
     5101 gtccgtctgg gaggcgcaaa agtgccgcac ctgatgccgg gggactcact gaacctgcag
     5161 acggctcagg acacggataa cggctactcc gtgtttgagc agtcactgtt gcggtatatc
     5221 gctgccgggc tgggtgtctc gtatgagcaa ctttcccgga attacgccca gatgagctac
     5281 tccacggcac gggccagcgc gaacgagtcg tgggcgcact ttatggggcg gcgaaaattc
     5341 gtcgcatccc gtcaggcgag ccagatgttt ctgtgctggc tggaagaggc catcgttcgc
     5401 cgcgtggtga cgttaccttc aaaagcgcgc ttcagctttc aggaagcccg cagtgcctgg
     5461 gggaactgcg actggatagg ctccggtcgt atggccatcg atggtctgaa agaagttcag
     5521 gaagcggtga tgctgataga agccggactg agcacctacg agaaagagtg cgcgaaacgc
     5581 ggtgacgact atcaggaaat ttttgcccag caggtccgtg aaacgatgga gcgccgtgca
     5641 gccggtctta aaccgcccgc ctgggcggct gcggcatttg aatccgggct gcgacaatca
     5701 acagaggagg agaagagtga cagcagagct gcgtaatctc ccgcatattg ccagtatggc
     5761 ctttaatgag ccgctgatgc ttgaacccgc ctatgcgcgg gttttctttt gtgcgcttgc
     5821 aggccagctt gggatcagcc gcctgacgga tgcggtatcc ggcgacagcc tgactgccgg
     5881 agaggcaccc gcggcgctgg cgttatccgt tgatgatgac ggaccacgac aggcccgcag
     5941 ttatcaggtc atgaacggca tcgccgtgct gccggtgtcc ggcacgctgg tcagtcggac
     6001 gcgagcgctg cagccgtatt cggggatgac cggttacaac ggcattatcg cccgtctgca
     6061 acaggctgcc agcgacccga tggtggacgg cattctgctc gatatggata cgccaggcgg
     6121 gatggtggca ggggcatttg actgcgctga catcatcgcc cgtgtgcgtg acataaagcc
     6181 ggtatgggcg ctggccaacg acatgaactg cagtgcaggt cagttgcttg ccagcgccgc
     6241 ctcccgacgt ctggtcacgc agaccgcccg gacaggctcc atcggcgtca tgatggctca
     6301 cagtaattac ggtgctgcgc tggagaaaca gggcgtggaa atcacgctga tttacagcgg
     6361 cagccataag gtggatggca acccctacag ccatctaccg gatgatgtcc gggaaacact
     6421 gcagtcccgg atggatgcaa cccgccggat gtttgcacag aaggtgtcgg catataccgg
     6481 cctgtccgtg caggctgtgc tggataccga ggctgcagtg tacagcggtc aggaggccat
     6541 tgatgccgga ctggctgatg aacttgttaa cagtaccgat gcgatcaccg tcatgcgtga
     6601 tgcactggat gcacgtaaat cccgtctctc aggagggcga atgaccaaag agactcaatc
     6661 aacaactgtt tcagccactg cttcgcaggc tgacgttact ggcgtggtgc aagcgacgga
     6721 gggcgagaac gccagcgcgg cgcagccgga cgtgaacgcg cagatcaccg cagcggttgc
     6781 ggcagaaaac agtcgcatta tggggatcct caactgtgag gaggctcacg gacgcgaaga
     6841 acaggcacgc gtgctggccg aaacccccgg tatgaccgtg gaaacggccc gccgcattct
     6901 ggcagctgca ccacagagtg cacaggcgcg cagtgacact gcgctggatc gtctgatgca
     6961 gggggcaccg gcaccgctgg ctgcaggtaa cccggcatct gatgccgtta acgatttgct
     7021 gaacacacca gtgtaaggga tgtttatgac gagcaaagaa acctttaccc attaccagcc
     7081 gctgggcaac agtgacccgg cacatacggc aaccgcgccc ggcggattga gtgcgaaagc
     7141 gcctgcaatg accccgctga tgctggatac ctccacccgt aagctggttg cgtgggatgg
     7201 caccaccgac ggtgctgccg ttggcattct ggcggttgat gctgaccaga ccagcaccac
     7261 gctgacgttc tacaagtccg gcacgttccg ttatgaggat gtgctctggc cggaggctgc
     7321 cagcgacgag acgaaaaaac ggaccgcgtt tgccggaacg gcaatcagca tcgtttaact
     7381 ttacccttca tcactaaagg ccgcctgtgc ggcttttttt acgggatttt tttatgtcga
     7441 tgtacacaac cgcccagctg ctggcggcaa atgagcagaa atttaagttt gatccgctgt
     7501 ttctgcgtct ctttttccgt gagagctatc ccttcactac ggagaaagtc tatctctcac
     7561 aaattccggg actggtaaac atggcgctgt acgtttcgcc gattgtttcc ggtgaggtta
     7621 tccgttcccg tggcggctcc acctctgaat ttacgccggg atatgtcaaa cccaagcact
     7681 tagcatggct ttctgaggct ttcgtgtagt tgctggtttt tacacttaat cttttgataa
     7741 taaagaataa gtttatctgg tgctttcact gaattttcct cgttatctgt gtgttgcaat
     7801 catctctgta ttgcagcttg tattgctttt tggggctaaa aatggctggc gagaacaaac
     7861 tgagcgacaa agcgcttaaa ggatatctgg ggaaacccag agaaaagcag atcaccattg
     7921 ctgatggaaa ggggctttct attcgtgtga gtactaaagg ggctgtgagc tttgttttct
     7981 tctacaggtt agcaggtggc cgggctgctc cggtctggct aacgttgggt aaatatcctg
     8041 atatgtcact caaacaggca agggaaaagc gcgacgagtg ccgtggttgg ttggctgaca
     8101 aacgtgatcc gcgtatccag attaagattc aggctgaaga acgcttaaag ccggttacag
     8161 tggaggatgc actaaattac tggtatgaaa attactgtaa ggtgcgtcgt aaaactcatg
     8221 ctgtaacgct tggcagattt cgaaagcata tctttcccta tatcggtcat ttgcccgtaa
     8281 atgacactca cctatatgaa tggctggact gttttgaccg aattaaacgt aatgcaccag
     8341 ttatggcggc gtatgttttt tctgacacta aattagctct tcgtttttgt cgggtacgcc
     8401 agtacgcgac gtgtgatgct ttaaaggatt tgcgcatgag tgatgtgggg cagattgcag
     8461 gtaagcggga tcgggttctg gatgaagccg aactcggcca gctctggaag gcaatttttg
     8521 tcgagcctga tttaaaacta atgtctgaat acacgcgaaa aatgtttgtg ctttgtacag
     8581 tatttggatg tcgaatgagt gaagcccgat tatcagaatg gagcgaatgg gatctcgaaa
     8641 gttgggtttg gactgtacca aaagatcact caaaaactgg tgttgaaatc gtcagaccag
     8701 tacctgaaat tctacgacag tgggtaacgg atgttcacga agagacaaaa catactggtt
     8761 atgtgctggg aagtctgcga attagagaaa gcgtaagcaa gattgggggg aaaatcggta
     8821 aacgtttggg ccatgaaaaa caatggtcac tacacgacct tagaagaacg ctatctactc
     8881 atctaagtga tctcggtgtt gaattttatg tagtagaaca actgttaggc catgcgctac
     8941 ctggcgtggc aggtgtttac aatcggagta agtttatggc taaaaaactg gatgctctgg
     9001 aactctggac tacatatctc aatagcatcg caggtgctga ttcaaaagtg acaatcctca
     9061 aacaaaaggt tggttaacat gaaaaaaatg gcaattgttg ataaaaaggg tctggagtac
     9121 attcctaaca ttgaccgaat gatccgtgag aaagaatgtc gggagctaac cactcttgcg
     9181 aacagcacac gctggaagct ggagaaggaa ggaaaatttc ctaagcggat caagattggt
     9241 tccactgctg ttgcatatcg tctttcagaa gtgcaggcat ggattcgagg tgagtgggta
     9301 gtttaaa
//
LOCUS       DAPHCV010000151         9149 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.152, whole genome shotgun sequence.
ACCESSION   DAPHCV010000151 DAPHCV010000000
VERSION     DAPHCV010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.152"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..425
                     /locus_tag="RH033_004155"
     CDS             <1..425
                     /locus_tag="RH033_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HDV2993261.1"
                     /translation="ALADWFYLNPEWLNGRVRYPLSRAGEWPATQDAFCQLISDSDNV
                     DIILWHSFPFAGVHSQEYCGVLFRKKKEINKAIIYPVLSLSPVRMNKEKENWFHTARN
                     LYAKTSVRTVTLRPAQAEYLMTGKILPAELFSIPLLPW"
     gene            514..1716
                     /locus_tag="RH033_004156"
     CDS             514..1716
                     /locus_tag="RH033_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HDV2993262.1"
                     /translation="MMMNMHITRTLIASAVLFSFNSTAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKAGPEDDFSLLIPSVGGQLSDPGNISDNADKISDDWKAFDRAID
                     SQSNVSQTAARLKTRLQDFRHTHADAQVGTSAVATLPGDTLAAALMLKSYGTVSVDGK
                     VSDADLNYLESIANSNGQDVDKDRLTSQAFARAALVTDVGIALATELETAGQKWSLGF
                     TPKFQRVDLFNYNVLVKNYDSSAFKGDRYHNTRNGINADIGASMDLDDNWTLGLVAQN
                     LLPRSIETKEVNGVTETFRIRPQVTAGVSWHNDMFTTALDVDLTPASGFTSDSKRQFA
                     AIGAEFNAWKWAQLRAGYRQNLASNDGSAFTAGLGISPFDVVHIDVAGIAGTDNNYGA
                     VAQLQFTF"
     gene            1856..2026
                     /locus_tag="RH033_004157"
     CDS             1856..2026
                     /locus_tag="RH033_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993263.1"
                     /translation="MFNKNKTHIENKAFMVEPFRDPTANECNVVIANDICIRVALSPS
                     EVLQPFSKNTNQ"
     gene            2075..2527
                     /locus_tag="RH033_004158"
     CDS             2075..2527
                     /locus_tag="RH033_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079808.1"
                     /note="Members of this family include DotD of type IVB
                     secretion systems and TraH of plasmid conjugative plasmid
                     systems, both lipoproteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotD/TraH family lipoprotein"
                     /protein_id="HDV2993264.1"
                     /translation="MNRMIPAGCLAVLCLNGCHLPPDNTAPTRKYGAASDVAVARHAQ
                     YQLWGQGIYSAALPERQSGGSLKAHSDRVSFDWEGDALELLNELARLRGQQFSYSGVR
                     LPLPVSLHVRDMTFADVLRITEAQTAWRATLSQSAGRLHLSFMTPEKK"
     gene            2524..3360
                     /locus_tag="RH033_004159"
     CDS             2524..3360
                     /locus_tag="RH033_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HDV2993265.1"
                     /translation="MKTHLCLLLSALLLTGCAGQHHRSSAVMTENAPPPDIRAWLTPS
                     GNQDDGISDTRRQMLTDAGRTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISK
                     EGWLPPVIDEAQDVAHIRPDQIRTASRVWTILRPERFVSNPPGWRDWLLKGLSTTATP
                     GTEGRVVPEDRAQRRLWENALRQGWQEGRLNADLTLEANQKRLTRDYRGMMLYALLWR
                     QGMITRPDVTEQMQTVTGNGQKLITGDRVRRLKTHAEFTLQKSRWRPLVSTEGEKNDI
                     TR"
     gene            3344..4492
                     /gene="traJ"
                     /locus_tag="RH033_004160"
     CDS             3344..4492
                     /gene="traJ"
                     /locus_tag="RH033_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HDV2993266.1"
                     /translation="MISPDDFGTFPFSRFTAEEFRHFFAWCARHRVSDIDLTGGSPVS
                     VSRFGRRVRSSVNPLPSSIMMALSDELFGREVLPRVLSGHPVDRTIQVSGDASGRYGL
                     QRGQRVRLRCHLIQGTSATEEKAISVTMRVIPSDIPDILTMNIEPDLLDAMLRKTGLG
                     FICGETGSGKSTLAAALYRHILKNFPDRKIVTYEDPVEYILGGENDLLPPHQAEIGRD
                     VESFAAGLRSAVRRNPDIIGIGEIRDYETADAAVQAGNTGHFCIGTMHTKSPGETFAR
                     LLGLFPPEIRDSMAAATLSLVQFILVQVLVRTNDGGRQAVREYIVITDELRNTLSGQS
                     HATWGHYIDEIIRQEKRRIRDQVLTMYQNDRIEKSEAALFIPAGEFPR"
     gene            4489..4779
                     /locus_tag="RH033_004161"
     CDS             4489..4779
                     /locus_tag="RH033_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDV2993267.1"
                     /translation="MKREGFWRDVTPPLNIYGVPLPFTMIYLLLFPYPSKTTFWVCSG
                     ILLFFMLLGYSGWTVRTLCIRLYSLLQGKQASGRPWWYRHRTELPREWHGPE"
     gene            4843..8859
                     /locus_tag="RH033_004162"
     CDS             4843..8859
                     /locus_tag="RH033_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDV2993268.1"
                     /translation="MKSRYRTWLAVPPEETEAVKNAVPPLNGRKAVAWDPEKKLWYAR
                     AGTELSLLERWLPRPQELSMDAGDPVTEFAQVLENAGLVIQGLPQMDGAIHRVATRDD
                     KKGAKSGAYKAYLDGRPAGWYRDYRSADDSPTNWVFSGGEQHDPLARLHLRAFAQQQR
                     DDNARKLQQQYNKQARYARSYINQLPQATEHEYLTRKGILAAPGVRLNNKNELVIPFS
                     NSRGEIRSYQRIPVTGGKDARILKDSEKTGNWFTFGTPENGRPLLFAEGYATAASLHE
                     ATGLPVLMTVDAGNMIDVAENARQIWTDSPFVFCADNDHQRKINKGVSSAQKAAASTH
                     GEVIIPAFTEAEKAQGLTDFNDLDASRGRDAFQRAMNAQLEYIGIPTPNNDTTDRREA
                     IVIGNLIFTPVKDEKPQVYPENRQSTAPEPAVSEQNHPYDIPAEETDNQAYEDDYAAY
                     ASLQSVTAGTASPNEPQVNAVSAIDSNAKNTSAATTDDRNVLAQEPNADATVTTTAHD
                     TGTAETDSPSEPAEIQPVQQASPEPSAKDKPQENVVSATDNDAENTSSATTALRSYDT
                     PETSVSDATGTQHTATTQPEEPQQVSMGSADDWSAFAEDLNADAPGNATVHSTDNAET
                     KTLSEPAGMQPVQQATPEPTEENKPQEEGVMSATDTGEESAAAAIDNDLNTVAEDLNA
                     DVTVMTTAHHTDTAETASPAEPAEIQPVQQATPEPTAEDKPKEERPVSATETSTANET
                     ARPETPSLFSRLRRFLTGGKSRTVEPPVIQENTLTEAETATEAAAPPSPPETSPEDTS
                     SAEPDTIIFAPRRPNSPQRHNLDEIIKNLEHEELPDNTALYRVDGEPAFLDKLNRLEM
                     VDGASADDKKVLAALAVATNFYGGVIELTGSDAFKQKAMQLIVEYDIKVRMKLPAQRA
                     ALEKLRKEKEMAHDAIVTHRPTPELNRQSPEETTMAQPAPPVSPKAEAEQTAGSTAET
                     PHPEAPVTAKAEAPRADRPEQPAAPSSAPEKAPVSAQPQQPDTPEEEPPGKLRPGESV
                     TAVLTNYGEKEYAPGKGRCFFVELTNRSGKREYWGNGLEELVKNHQKGDPVTLTLQAR
                     ETFRLSGETRDRIRNIWAITPVSNGITVNNDQPHAGQHTLEFPMATFSQVMSQMQQHW
                     PQIMQEVRSPKNPPNNLYIGEDRQPASAPQDQSMRLPLAGNAPNELIPAIASADKDNR
                     QLNLLLVHSADDHLQGVVRLNGTLYPALATPSADNRQLVINALTDNGLQFAGYGEAVN
                     HDENTHQRPSPQIMQFHLKQQDSPLFAAIHKPEEQPDKLFRSLGFEQTWKEWSDSQKA
                     EDRQEKTLQQAQSHSPGL"
     gene            8876..>9149
                     /locus_tag="RH033_004163"
     CDS             8876..>9149
                     /locus_tag="RH033_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDV2993269.1"
                     /translation="MNMKKTLLVILTSISLASPAAVAATCRAGAAAEAGSKAAWERAR
                     TASQAWSQRENDVASSLQSCLSRIKDVSITLPQFPSLDKILRDLENQ"
BASE COUNT         2361 a         2650 c         2300 g         1838 t
ORIGIN      
        1 cggctctggc tgactggttt tatctcaacc cggaatggct gaacggcagg gtacgttatc
       61 ctttaagccg tgccggtgag tggccagcca cacaggatgc gttctgccag cttatttcag
      121 acagcgataa tgtggatatt attctctggc acagttttcc ttttgccggt gttcattcac
      181 aggaatattg cggtgtactg ttccgcaaga aaaaagaaat taataaagcc atcatttacc
      241 ctgtactttc tttatcgcca gtaagaatga ataaggaaaa agaaaactgg tttcacactg
      301 cccgcaattt atatgcaaag acatccgtcc ggaccgtaac gcttcgtccg gcacaggcag
      361 aatatctgat gacaggcaaa atattacctg ctgagttatt cagtataccg ttattaccct
      421 ggtaataacg cagagaaaaa ccaataaatt tttaatggca ccgtcgtgtc ataccgttgc
      481 tgtacccaaa acacctcaaa ccaggaaact gaaatgatga tgaatatgca cattacccga
      541 actctcatcg catctgctgt tttattttcc tttaattcaa cagccgcaac ttcatatttt
      601 gaagcccgca atgatgcaat ggggggaacc ggggtagcct cctctcacta cggtgtggca
      661 cctctggcaa acccggccct gctgacaaaa gccggtccgg aagatgactt cagtcttctg
      721 attccgtccg ttggtgggca attgtccgat ccgggtaata tcagtgacaa cgcagacaaa
      781 atcagtgatg actggaaagc ctttgatcgt gccatcgaca gtcagagcaa cgtgtcgcag
      841 actgctgcac gtctgaagac gcgacttcag gacttccgtc acacgcatgc tgacgcacag
      901 gtgggaacat ctgctgtggc gactcttccc ggtgacaccc tcgctgccgc tctgatgctg
      961 aaaagctacg gtacggtcag tgtcgacggt aaagtcagtg atgccgacct gaactatctg
     1021 gaaagcattg caaacagtaa cggtcaggat gtggataaag acagactgac ctctcaggct
     1081 tttgcccgtg ccgcactggt caccgatgtg ggtattgctc tggctactga actggaaacg
     1141 gccggacaaa agtggtctct gggcttcaca ccaaaattcc agcgtgttga cctgtttaac
     1201 tacaacgtgc tggttaaaaa ttacgacagt agcgctttca aaggcgaccg ttaccacaac
     1261 accagaaacg gcattaacgc tgatatcggg gccagcatgg acctggatga caactggaca
     1321 ctgggtctgg tcgcacaaaa cctgctcccc cgcagtattg agacaaaaga agtgaacggt
     1381 gtcactgaaa ctttcaggat ccgcccgcag gtgactgccg gtgtctcctg gcacaacgac
     1441 atgttcacta cagcactgga tgttgacctg accccggcca gtggcttcac atcagacagt
     1501 aaacgtcagt ttgctgccat tggtgctgaa tttaatgcct ggaaatgggc acagttacgt
     1561 gcaggctacc gccagaatct ggccagcaat gatggcagtg cgttcaccgc cgggctgggt
     1621 atctcaccat ttgatgtcgt ccatattgat gtggcgggta tagcaggcac agacaacaac
     1681 tacggtgccg ttgcacaact gcagttcaca ttctgatcat ctctgccccg gtactgaccg
     1741 gggtatccag acctgcatcc tcgcgttttc cgaccatcaa acgacacagc cagacctgta
     1801 gtgctgcctc tgatctggct gttatctgac atatcatttt cgcatgagga aatgcatgtt
     1861 taacaaaaat aaaacgcata tcgaaaacaa agcctttatg gtggaaccgt tccgggatcc
     1921 gacagctaac gaatgtaatg tcgtgatagc aaacgatatt tgtatccggg tagcactgtc
     1981 accctctgag gtgctccagc cattcagcaa aaacacgaac cagtaaatcc tccccacaac
     2041 gctttcttct ttcactgctc aggaaaaata caccatgaac cgtatgatac ctgcgggatg
     2101 tctcgccgta ctctgcctga acggctgcca tctccccccg gataatacag ctccgaccag
     2161 aaagtatggt gctgcctctg atgtcgccgt cgcccggcat gcgcagtatc agctatgggg
     2221 gcagggaatt tactctgcag cccttccgga gcgtcagtcc ggcggcagtc tgaaggctca
     2281 cagcgaccgt gtttcctttg actgggaagg cgatgccctg gaattgctca acgaactggc
     2341 ccgtctgcgc ggccagcagt tcagctacag cggcgtccgt ctccccttac ccgtctcact
     2401 gcacgtccgc gacatgacct ttgctgatgt ccttcgtatc actgaagccc agactgcctg
     2461 gcgggcaaca ctctcgcagt cagccgggcg tttacatctg tcttttatga cgccggagaa
     2521 aaaatgaaaa cacacctgtg tctcctgctg tctgctctcc tgcttacagg ctgtgccgga
     2581 cagcatcacc gttcttctgc cgtcatgaca gaaaacgcac cgcccccgga tatccgtgca
     2641 tggctgacgc cctccggcaa ccaggatgat ggtatttcag atacccgtcg tcagatgctc
     2701 acggatgccg gcagaacgct gggattccgt ggtggaaaag cacagcgctc atgggaactg
     2761 attcaggcac tgaatgcccg ggaaagtacc ctgaatgccc tgtatgattt ccggcctctc
     2821 atcagcaagg aaggctggct gcctcctgtt atcgatgaag ctcaggatgt tgcacacatc
     2881 agaccggacc agataagaac agcctcccgt gtctggacca tcctccgccc ggaacgcttt
     2941 gtcagtaatc cacctggatg gcgtgactgg ctgctgaaag gactctcaac caccgccaca
     3001 ccaggcactg aaggccgggt tgtgccggaa gaccgggcac aacgcagact atgggaaaac
     3061 gccttacgtc agggatggca ggaagggcgc ctcaacgcag atctcacact ggaagctaac
     3121 cagaagcgcc tgacccgtga ttaccggggc atgatgcttt acgcccttct gtggcgtcag
     3181 ggcatgatca cacgtccaga tgtcacagag cagatgcaga cggttaccgg caacgggcaa
     3241 aaactcatca ctggcgatcg cgtccgccgc ctgaaaacac atgctgaatt tacgctgcag
     3301 aaatcgcgct ggcgccctct tgtcagcacc gaaggagaga aaaatgatat caccagatga
     3361 cttcggtacc ttccccttct cccgttttac ggcagaagag tttcgccatt tttttgcctg
     3421 gtgtgcccgc caccgcgtca gtgatattga tctgaccggc ggttcaccgg tatccgtcag
     3481 tcgctttgga cggcgggttc gctcatccgt aaatcccctt ccgtcttcca tcatgatggc
     3541 actgagtgat gagctgtttg ggcgggaggt tcttccccgg gttctgtcag gtcatcccgt
     3601 ggatcgtacc attcaggtca gcggagatgc ctccggccgc tatggcctgc agcgaggtca
     3661 gcgtgttcgc ctgcgctgcc acctgataca gggaacatcg gcaacagaag aaaaagccat
     3721 ttccgtcacc atgcgggtga tcccgtcaga tattccggac atcctcacta tgaatattga
     3781 accggatctg ctggatgcca tgctcagaaa aaccggcctg ggcttcatct gcggagaaac
     3841 cggctccggg aaatccacac tggcggcggc actgtatcgc cacattctga aaaatttccc
     3901 cgatcgcaaa atagtgacct atgaagatcc ggtggaatac attctgggcg gagagaatga
     3961 ccttctgcct ccccatcagg ccgaaatcgg gcgcgatgtg gaaagttttg ctgcaggact
     4021 tcgctctgcc gttcgccgta atccggacat cattggtatc ggtgaaatca gggattatga
     4081 aacagcagat gccgcagtcc aggcgggtaa taccggacac ttttgtatcg gcaccatgca
     4141 cacaaaatca cccggagaaa cctttgcccg cctgctggga ttgtttcctc ctgaaatcag
     4201 ggattcgatg gctgcagcca cgctttccct cgtacagttc attctggtgc aggtactggt
     4261 cagaacaaat gacggcggca ggcaggctgt ccgggaatac atcgtcataa ctgacgaact
     4321 gcgcaatacg ctctccggac aatcccatgc aacatgggga cattacattg acgaaatcat
     4381 tcgtcaggaa aaacggcgga taagggatca ggttctgacc atgtatcaga acgaccgcat
     4441 tgagaaatca gaagcagcgc tcttcatccc ggccggagag ttcccccgat gaaacgcgaa
     4501 ggcttctggc gggatgtaac accaccgctc aacatttatg gtgtccccct gccgtttacc
     4561 atgatttacc tgctgttgtt tccgtatccg tcaaaaacga cattctgggt gtgcagcggc
     4621 attctgctgt tttttatgct cctgggatat tccggctgga cagtccgcac cctctgtata
     4681 cgtctgtaca gcctgcttca gggtaagcag gcatcgggac gtccctggtg gtaccgacac
     4741 cgcacagaat taccccgtga atggcacgga ccagaatagc cctcttttgt ccatcatccc
     4801 ggcgtatcac aacgccctca tatttaatcc ccggagaacg acatgaagtc cagatatcgt
     4861 acctggcttg ctgtcccgcc tgaggagaca gaagccgtaa aaaatgcagt ccccccgtta
     4921 aatggccgca aagctgtcgc atgggatcca gaaaaaaaac tctggtatgc ccgggcgggt
     4981 accgaacttt cactgctgga gcgctggtta ccccggccgc aggaactgtc gatggatgcc
     5041 ggcgatccgg tcaccgaatt tgctcaggtc ctggaaaacg ccggactggt tattcaggga
     5101 ttgccacaga tggatggcgc catacacaga gtcgccaccc gggatgacaa aaaaggggcc
     5161 aaaagtggcg catacaaggc ctatcttgat gggcgccccg ccggctggta ccgcgactac
     5221 cggagcgccg atgacagtcc cacaaactgg gtattttccg gcggagaaca acacgatcct
     5281 ctggccagac tgcatctgcg ggcattcgct cagcaacagc gcgatgataa cgcacgtaaa
     5341 ctgcagcaac agtacaacaa acaggcccgg tatgcccgct cctatattaa ccagttacca
     5401 caggcaacag agcatgaata cctgacccga aaagggatcc ttgccgcacc gggcgtccgt
     5461 ctgaataaca agaatgaact cgtcatccct ttcagtaaca gccggggaga aatacgttca
     5521 taccagcgta ttcctgtcac cggcgggaag gatgcccgca ttctgaaaga ctcagagaaa
     5581 accggtaact ggttcacctt cggaaccccg gaaaatggcc gtcccctgtt atttgcggaa
     5641 ggttacgcaa ctgcagcctc cctgcacgaa gccaccggcc ttccggttct gatgacagtt
     5701 gatgccggca atatgattga cgtggcagaa aatgcccgcc aaatctggac tgacagcccc
     5761 tttgtcttct gtgccgacaa cgaccatcaa cggaaaatta acaaaggtgt ttcaagtgcc
     5821 caaaaagctg ccgcaagcac gcatggcgaa gtcattattc cggcctttac ggaggcggaa
     5881 aaagcacagg gactgaccga tttcaatgac ctggacgcca gcaggggacg agacgccttt
     5941 cagcgagcca tgaacgcaca gttagaatat atcggtatcc ccacgcccaa caatgacact
     6001 actgaccgcc gtgaagctat cgtgatcggc aacctgatat tcacaccggt aaaagatgag
     6061 aagccacaag tgtaccctga aaacagacag agcacagccc ctgaaccggc agtgtcggaa
     6121 cagaatcatc cttatgacat ccctgcagaa gagacagaca atcaggcata tgaagatgac
     6181 tatgcagcat atgcatccct gcagtcggta actgcaggga cagcctcccc gaatgaaccg
     6241 caggtgaatg ctgtttctgc cattgacagc aacgcaaaga atacctctgc cgccaccact
     6301 gacgaccgaa atgtattagc acaagaaccc aatgctgacg ccacagttac gaccacggct
     6361 cacgacaccg gcacagcaga aacagattca ccttctgaac ctgcagagat tcagccagtg
     6421 cagcaggcca gtcctgaacc atcggctaaa gataaaccac aggagaacgt agtgtctgct
     6481 actgataatg acgcagagaa cacctcttcc gccaccactg ccctccggag ttacgacaca
     6541 ccggaaacat ccgtctccga cgcaaccggg acacagcaca ccgccacaac gcaacctgaa
     6601 gaaccacagc aggtctccat gggcagcgct gacgactgga gcgcatttgc ggaagacctc
     6661 aatgctgatg ctccggggaa tgccactgtt cacagcaccg acaacgcgga gacaaagaca
     6721 ctttctgaac ctgccgggat gcagcctgtt caacaggcaa cccctgaacc aacggaagaa
     6781 aataaacctc aggaagaggg cgttatgtct gccactgata caggtgaaga gagtgccgct
     6841 gccgctatcg ataacgacct gaacacagtt gcggaagacc tcaatgctga tgttactgtg
     6901 atgaccacag ctcaccacac cgatacagca gaaacagcct cacctgctga acctgcagaa
     6961 attcagcccg tacagcaggc aacccctgaa ccaacggcag aagataaacc gaaggaagag
     7021 cgtcctgtgt ctgccactga gaccagcaca gcgaatgaaa cagcccgccc tgaaacacca
     7081 tcgcttttca gccgcctgcg ccgttttctg accggtggaa aatcacgtac cgtcgaacct
     7141 cccgttattc aggagaacac gcttacggag gccgaaactg cgacagaggc cgcagcacca
     7201 ccgtcaccac ctgaaaccag cccggaagac acatcgtcag cagaaccgga cacgattatc
     7261 tttgcgcccc gccggcccaa cagtccgcag cggcacaacc tggacgagat tattaaaaat
     7321 cttgagcacg aagagttacc cgacaatact gcgctctacc gcgtcgacgg cgaaccggca
     7381 ttcctggata aactcaaccg tctggaaatg gtggatggtg ccagtgctga tgacaaaaag
     7441 gtcctggctg cccttgctgt ggccaccaac ttctatggcg gcgttatcga gctgaccggc
     7501 agtgatgcct ttaaacaaaa agccatgcag ctgattgtcg aatatgacat caaggttcgc
     7561 atgaaactcc cggcccagcg ggcagcactg gaaaaactcc gcaaggaaaa agagatggcg
     7621 catgatgcca ttgtcaccca cagacccacg ccggaactca accgccagtc accggaagag
     7681 acaacaatgg cccaaccggc tccccctgtg tcaccgaagg cagaggctga acaaacagca
     7741 ggcagtacgg ctgaaacacc gcatccggaa gcgccggtca cggcaaaagc tgaagccccc
     7801 cgggccgaca gaccggaaca acccgccgcc ccgtcgtctg cgccagaaaa agcccctgtc
     7861 agtgcacagc cgcagcaacc cgacacaccg gaggaagagc cgcccggcaa gctcagaccc
     7921 ggtgaaagtg tgactgccgt cctgacaaac tacggagaaa aagagtatgc gcccggaaaa
     7981 gggcgctgct tttttgtgga gctcaccaac cgcagtggca aacgggaata ctggggaaac
     8041 ggcctggaag aactggtcaa aaaccatcag aagggtgacc ccgtcacact gacactgcag
     8101 gcgagagaaa cattccggct gtccggtgaa acgcgggatc gtatccgtaa tatctgggcc
     8161 ataaccccgg tcagtaacgg cattaccgtt aataatgacc aacctcatgc gggtcagcat
     8221 accctggagt ttccgatggc aacattcagc caggtgatgt cacaaatgca acaacactgg
     8281 ccacagataa tgcaggaagt tcgttccccc aaaaatcctc ccaataatct gtacattggt
     8341 gaagacaggc aacctgcatc agcacctcag gatcaatcaa tgcgactccc cctcgctggt
     8401 aatgccccca atgaactgat accagccatc gcctctgccg ataaggacaa tcgtcagttg
     8461 aatcttctgc tggtccacag cgcagatgat catctgcagg gcgtggtccg cctgaacggc
     8521 accctttacc cggcactggc caccccgtca gctgacaacc gtcagctggt catcaatgcc
     8581 ctgacggata acggtctgca gtttgccggt tacggtgaag ctgttaacca tgatgagaat
     8641 actcatcaac gtccgtcccc acaaataatg cagtttcacc tgaagcaaca ggactccccg
     8701 ttgtttgccg ccatccacaa accggaagag caaccggaca agcttttccg ctctctgggt
     8761 tttgagcaga cctggaaaga gtggtctgac agccaaaagg cggaggacag gcaggaaaaa
     8821 actctgcaac aggcacaatc ccacagcccc ggcctgtgag ccggtaagga cgaccatgaa
     8881 catgaaaaaa acgctgctgg tcatactgac cagcatctcg ctggcttctc cggctgccgt
     8941 ggcagcaacc tgtcgggcag gtgctgccgc agaagccggc agcaaagccg cctgggagcg
     9001 ggcgcgcaca gccagccagg catggtcaca gcgagaaaac gacgtcgcct cctccctgca
     9061 gtcctgcctg tcccgcatta aggacgtcag catcactctt ccacaattcc ccagcctgga
     9121 caaaatcctt cgcgatctgg aaaaccagg
//
LOCUS       DAPHCV010000152         9079 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.153, whole genome shotgun sequence.
ACCESSION   DAPHCV010000152 DAPHCV010000000
VERSION     DAPHCV010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.153"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            203..1027
                     /gene="gadX"
                     /locus_tag="RH033_004164"
     CDS             203..1027
                     /gene="gadX"
                     /locus_tag="RH033_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HDV2993270.1"
                     /translation="MQPLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIQV
                     DKCVENFVLVSRDTLSLFLPMLKEEALKLHAHKKVPSLLVHHCTRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDTQFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEGTSYSQLLTECRMQRALQLIVIYGVSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQELPNCGPAASMAAQGNFYGTDRSAEGIRL"
     gene            1396..2124
                     /gene="gadW"
                     /locus_tag="RH033_004165"
     CDS             1396..2124
                     /gene="gadW"
                     /locus_tag="RH033_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HDV2993271.1"
                     /translation="MTHVCSVILVRRSFDIHHEQQKISLHNESILLLDKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQIPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNDSG
                     VNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2487..5600)
                     /gene="mdtF"
                     /locus_tag="RH033_004166"
     CDS             complement(2487..5600)
                     /gene="mdtF"
                     /locus_tag="RH033_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HDV2993272.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIRLAAGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIK
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVIGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALMAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5625..6782)
                     /gene="mdtE"
                     /locus_tag="RH033_004167"
     CDS             complement(5625..6782)
                     /gene="mdtE"
                     /locus_tag="RH033_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HDV2993273.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGTLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            6842..7120
                     /locus_tag="RH033_004168"
     CDS             6842..7120
                     /locus_tag="RH033_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993274.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYIYQGNKVVFASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7121..7648)
                     /gene="gadE"
                     /locus_tag="RH033_004169"
     CDS             complement(7121..7648)
                     /gene="gadE"
                     /locus_tag="RH033_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HDV2993275.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8447..9019)
                     /gene="hdeD"
                     /locus_tag="RH033_004170"
     CDS             complement(8447..9019)
                     /gene="hdeD"
                     /locus_tag="RH033_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HDV2993276.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKKQ"
BASE COUNT         2426 a         2112 c         2100 g         2441 t
ORIGIN      
        1 ggattatttg cttactatta atttccctgc gttcttaaac acgtttttgt attataaatc
       61 cagtcatcct gcccgtagaa catcacacat tatcatcctg ttctcccgct tcgtttaaat
      121 ttatttatca atcaatttga cttaagaggg cggcgtgcta cattaataaa cagtaatatg
      181 tttatgtaat attaagtcaa ctatgcaacc actacatggt aattgtctaa ttgcgtacgc
      241 aagacataaa tatattctca ccatggttaa tggtgaatat cgctatttta atggcggtga
      301 tctggttttt gcggatgcaa gccaaattca agtggataag tgtgttgaga atttcgtatt
      361 agtttcaaga gacacgcttt cattatttct cccgatgctc aaggaggagg cattaaaact
      421 tcatgcacat aaaaaagttc cttcattact cgttcatcac tgtaccagag atattcctgt
      481 ttttcaggaa gttgcgcaac tgtcgcagaa taagaatctt cgctatgcag aaatgctacg
      541 taaaagggca ttaatctttg cgttgttatc tgtttttctt gaggatacgc agtttatacc
      601 gttacttctg aacgtgttgc aaccgaatat gcgaacacga gtttgtacgg tcatcaataa
      661 taatatcgcc catgagtgga cattagcccg aatcgctagc gaattgttga tgagtccaag
      721 cctgttaaag aaaaaattgc gcgaagaagg tacgtcatat tcacagttgc ttactgagtg
      781 taggatgcag cgtgctttac aacttattgt tatatatggg gtttcaatta agcgagttgc
      841 agtatcctgt ggatatcaca gcgtgtcgta tttcatttac gtttttcgaa attattatgg
      901 gatgacgccc acagagtatc aggagcgatc ggcgcaggaa ttaccgaact gtggcccggc
      961 ggcaagtatg gctgcgcaag ggaattttta cggcactgac cgttctgcgg aaggaataag
     1021 attatagagt tttactcaga cataaaaaaa acccggcata ggggaccggg aagaggatag
     1081 tctgccgtct ccagactaat aaaccgttat aacactccct gttggcacgg gaaactttgt
     1141 gctctcagta agttaaatat aacttttact ggaaataaga tcagccattt ttttataaac
     1201 ataagctata cgctgtgcga aaatatattc gtgttgcatt tacttattat caattaactg
     1261 ttatgcaaaa ctactttgtg gataaatttt ggtcctacca aatctggcag tttttgcgct
     1321 aagaaacagt caggcatcat ttcattagta tactgaaatt gaaataatcg cagcaatgaa
     1381 atataaggga tagtcatgac tcatgtctgc tcggtgatcc tcgttcgtcg ttcattcgat
     1441 attcatcatg aacagcaaaa aatatcgttg cataacgaga gtatcctgtt actggataaa
     1501 aatttggcag acgattttgc gttttgttca ccggatacac gacgactgga tatcgatgag
     1561 ctgacagttt gccattactt acaaaatatt cgtcagatac cacgcaattt agggttacat
     1621 agcaaagacc gtctgttaat taaccagtca ccccccatgc cgctggtgac ggcgattttt
     1681 gatagtttca atgactccgg ggtaaattcg ccgatattga gcaaaatgct ctatctttcc
     1741 tgtttatcga tgttttctca taagaaagaa ctgatcccct tacttttcaa tagtatcagt
     1801 actgtttcag gaaaagttga acgccttatt agctttgata tcgccaaacg ttggtatctg
     1861 cgcgatattg cggaaagaat gtataccagc gagagtctca tcaaaaaaaa gttgcaggat
     1921 gaaaatacct gtttcagtaa aatattactc gcctccagga tgtcgatggc cagacgatta
     1981 ctcgagttac gtcaaattcc tctgcatact attgcggaaa aatgtggcta tagcagtacg
     2041 tcgtacttta taaacacatt tcgacaatat tatggtgtaa cgccacatca gtttgcgcaa
     2101 cattcgccag gtaccttttc ctgacatatt ttgcatttga atattggtca ggatctcaca
     2161 cctgcttcat gtgaaactct tccctgatga tttctgccgg gctaccggct agttctcttt
     2221 cgcagtttgc caggcggtat aggtattttc aggaggaaaa gactcggcat gtttgggatt
     2281 attaagctga caattcatac gattacggga atgtgggtca gtattgtgtt gtttaaattg
     2341 atgacgaatg gctggagtgg tttctacttt cagtgctgtg tactaagcct ggtattcctg
     2401 acggttagct ggttgttgag tggtgaatgg ttagcaggaa agagtaaggc tgaaccctca
     2461 cgttcaacct tactctcatt tacacgttac gcttttttaa agcgggcaaa gagatgttcc
     2521 actacaacga aaaagaccgg aacgaagtaa attgccagca ctgttgcggc aaacatcccg
     2581 cccatcacgc cggtacctac cgcgttttgc gcgccagaac cggcaccatg actgataacc
     2641 agcggcagca cgccgagaat aaaggccaga gaggtcatca ggattgggcg taaacgcatc
     2701 cgcgccgctt cgatgattgc ctctatcggc gttttccctt ctttctgcat catctcaacg
     2761 gcaaattcga caatcaggat ggcgtttttg gcggaaagcc cgatggtggt cagcaaacca
     2821 acctggaagt agacgtcatt acttaagccg cgtagatcgg tggccattaa tgcgccaacg
     2881 acgcctaacg gtacaaccaa catcaccgag aacggaattg accagctctc ataaagtgcg
     2941 gcgagggcga ggaacaccac gaccagtgaa atggcataca gcgcgggagc ctgatttgag
     3001 gataacgctt cctgatacga cagtccggtc catgagtagc cgacgcctgc cggaagttta
     3061 gcgaccaggt ctgccataaa tttcatggcg tcaccggtac ttttcccggc cgccgcttca
     3121 cctaaaatct ccattgacgg gatgccgttg tagcgttcca gtcgcggtga accataggtc
     3181 cattcggtag acgaataggc agaaagcggt gccatcgtgc cagaggcgtt gcgtacatac
     3241 cattggttga tgttatccgg caacatacgg aacggcgtgc ctgcctggac atacactttt
     3301 ttcacccgcc cctggttgag gaagtcgttc acgtagctgc tgccgaaggc ggtggaaatt
     3361 gtctggttga tatcagacag cgccaccccc atagcctcgg ctttcgcagc gttgacgttc
     3421 actttgaaca tcggcgtatc ttccaggccg ttcgggcgta ccccgatgac ctgattcggt
     3481 gattgcgctg ccagtgataa cagctcgttt cgcgcctggg tcagtttttc gtgccccaga
     3541 ttgccgttgt ccagcagttc catatcaaaa cctgacgcgg tacccagttc agccaccgcg
     3601 ggtaagttga acgggaagac gacggcttta ttgatactgc ttaacgcaat cattgcccgc
     3661 tgaatgatcg cggtaaccga gttttcctca ccgacacgtt cagaccacgg cttgagactg
     3721 ataaacgcca ggccgttgtt ttgcccctgg ccgctgaagc caaagccgcc aacggtaaac
     3781 accgactgaa cattatcttt ctctttagtc agataataat ccgtcacctg ttgcagcact
     3841 ttcgtggtgt taaccatggt ggcaccggaa ggtaactgcg cggtggtcat aaataccccc
     3901 tgatcctctt ctggtaagaa agaggtcggc gtacgcagga acagcaccgc catcccggcg
     3961 caaatcagca ggtagaccac catgtagcga ccggtacaac gcaacagcga gcgggtgcta
     4021 tcggtatagt gctgagttga tttttcaaac agcgtgttga agcgtgcgaa cagggcgtta
     4081 ggtttgtgac cgccttccgg cgcggctttc agaatggtgg cgcacagggc aggggtcagg
     4141 ctcattgcca caaatactga aagcagcatg gaggagatca gcgtgatgga gaactggcgg
     4201 tagatctccc cggttgcacc gctcataaag gccatcggca taaacactgc ggaaagaaca
     4261 acggcaatcc cgaccagcgc acgttggatc tgccccatcg atttatgcgt cgcttccttc
     4321 ggcggtagct tatcttccgc aatgacacgc tcgacgttct ccaccaccac gatggcgtca
     4381 tccaccagta accctatcgc cagcaccatc ccgaacatag ttaacgtgtt gatggtgaaa
     4441 ccgaccgccg acaagatcgc aaacgtcccg agaataacca ccggtacggc aatcgtcggg
     4501 atgattgtgg cgcggaaatt ctgcaaaaac agatacatga ccaggaagac gaggatgata
     4561 gcctcaacca gcgttttgaa aacttcctga atagaaattt tgataaacgg tgtggtgtcg
     4621 taaggataaa ccgtcttcag gcttgcaggg aaataagccg ataaacggtt cagttcctct
     4681 ttgactgccc gcgaggtatc cagggcgttt gctccggcag ccagtctgat ggcgatcccg
     4741 gcagcaggtt tgccgttata gcgcgccacg gtggaataat cttccgcccc aagttcgacg
     4801 cgggcgacat cacgcagcag cacttgcgag ccatcttgct gaactttcaa caggattttg
     4861 ccaaattctt ccggcgtttg cagacgcgtc tgcacaatga tcgaggcgtt cagttgctgg
     4921 tctgccgcct gtggcatgcc acccagttga ccaccggaaa tctggttgtt ttgcacctta
     4981 atctgggaaa taacatcgga aggtaccagg ttatatttat tgagtttttg cgggtccagc
     5041 cagatacgca tggcgtactc ggaaccaaag agctgtacgc taccaacgcc cgcggtacgg
     5101 cttagcgggt ctttgatatt agacgctaca tagtccgcga tatcgtactg gttgaggctg
     5161 ccgttatcag aaataaatgc cgctaccatc aggatattac tgctcgactt atcgacgcta
     5221 atcccctgct gctgcaccgc ttcaggtaat gaaggcatag cgagttgcag tttattttgc
     5281 acctgaacct gtgcgatatc aggcgatgtc cccgtctcga aggtcagagt gatggaggca
     5341 ttacctgccg catcgctggt tgaagacatg tacatcaggc catcaagccc attcatattt
     5401 tgctcaatca cctgagtgac cgagtcttct accgtttgcg catcggcacc tggataggta
     5461 gcgctgacgg taatggtcgg tggcgcaatc tgcggatact gcgcaaccgg taagttcatg
     5521 atcgccagac cgcctgcaag catcataata atggcaagta cccaggcaaa aaccgggcga
     5581 tcaataaaat agttagccat gaaagtcccc ttaagcctgc aacgttattg tttcgattcg
     5641 gtgctggcgt tttcctggct ggaggaaatt gctcgtgctt tgatacccgg acgaatgcgt
     5701 tgcaaaccgg aaacgatcac ccgatcgcca gcctgcaagc cagaggtgac aacccactgg
     5761 tcgccgatgg ctttgctggc ttcgatttcg cgtagctgca cgacatcgtc tttatccaga
     5821 atgagcgccg ttgctttacc ctgggcgttg tgggtgacgc cttcctgcgg caccagtaat
     5881 acattctggc ggctaccttc atccactaat gccgtgacat acatgccagg cagcaagtca
     5941 ccatttgggt tggggaaaat cgcccgtaac gtcacggagc ccgtggtttc atccaccgtc
     6001 gggtcggaga atttcagcgt gccggtctgg ctgtaacgtt taccattttc cagattgagc
     6061 tgtactggcg tactgccctg aacctgtttg atttgcccac tggcgacctc ttctttcatg
     6121 cgcaagaaat cctgcaccga ctgtgtgaga tcgacataaa tcgggtccag acgttgtacg
     6181 gtaaccagcg aatctgcctg attagcggta acgagtgcgc cgacggtcac cgacgatttc
     6241 ccgctgacgc ccgtaatcgg cgaggtgaca ttcgcgtatt gcaggttgat cgtcgcctgt
     6301 tcaacagccg ctttggcgac ggtgacattg gcttctgctt cattcaactg ggtgcgcgcg
     6361 gtgtcgtaat cctgacggct aacgtagttg gtcttcagca acgatgcctg gcggttaaag
     6421 gtgatgcggg cattgctggc ggtagagagc gctttcgcca gcgtgccttt ggcggagttc
     6481 agctcggcct gtaaaggtgc aggatcaatc tgatacagcg aatcgccctg gttcacttta
     6541 tcgccttcga taaagttgcg tttaatgata ataccgccca cctggggacg tatctcggca
     6601 acttcataag gaacggttct accgggcaat tcgctcaaca cattgaccga accgggggag
     6661 agtgtgacga caccgacctc aggcgtcatg gctgcggcgt tttccgccga tttgtcatcg
     6721 caggcggtga gcatcgcgcc gcagaataac aacggtatta acagctttct tcttctgttc
     6781 attttagtcc ctgaaaattc ttgagaaata acggttcttt aaattgcgcg cgaggagaga
     6841 tatgagggat ttacagacca gcggcatagt ggggttgtca gccagtaagg tgggatacag
     6901 gcacagtgat cgacatggtg aggtcaacga caaaaaaaat gttgccgttc tgccaacagt
     6961 tcctgccagc attcgggcaa cgaagggttc tactggtgga tacatatacc aggggaataa
     7021 agtcgttttc gcctctcctt tagtaatcac cgggggtaat gaaatcagca tctgcatccc
     7081 tcgtcatgcc agccatcaat ttcagttgct tatgtcctga ctaaaaataa gatgtgatac
     7141 ccagggtgac gatgtcgctc atacgtctta gtttcagttt gtacataatg ttgaatttct
     7201 gaatctttaa tgtttgttgc gaaatattga gcgtcgacgt gatattgctt tcatttttat
     7261 ttttcagaat cagttgcata attttgaact gattatgtga aaaacataat tgtgaattct
     7321 tatggggcaa gtgtttacca taagtaatct caagaagatt tttgatactt tctttgcggc
     7381 tgacaaaata aataggcgct ttagctttta gtttactgat gtgataaggt gccaaaacaa
     7441 taattctctc ggcatctaat ttctccagaa atttaatcgc ttcttcatca aggatatgat
     7501 tgtgataggt atcaatgata accttgaagg gtttattgcc tacttttttg atctctgaca
     7561 gggaattgat ttttatcatt tcgtgattat ctttcaactg ccaaaagccc tgtaaaagaa
     7621 aagaatcttt cgtcatgaga aaaatcataa cttgctcctt agccgttatc gtttcgaaca
     7681 tgtcatcctt ttcattattt acatccttgt ccgaatcgtt gttcaatata gtaaacgcct
     7741 ataagaactt tgcccttcgt gaactaatac gtctattaac actaatcaat acttcagatt
     7801 tatccatcac gcactccata tattggtcct aagtaagatt cctggttgtt atcagtttgt
     7861 aactagtaaa caaaaggtta acttttgcgg attactgccc aagaataagt tatcaataac
     7921 cagtttgtga tctctgaaga atattactaa agttaaaatg taatccgatt taaatatcga
     7981 gtctccttgt ttcgacttag gctggcaatt ggattgccag ctttctttta acgcattgaa
     8041 attacctgat taaataactc attcatgtct tcaggttttt atatagcgtc tggagacacg
     8101 gatatttatg caatgtaatc aacgccagtt gcaaaaaaat gaacccggat tacttattta
     8161 accgcccata gcatcaacgc ttacggaact ctttgttcac tttcccacag cacttttaaa
     8221 acattaaacc tgaccacgga aaatatcagc catgataatg tttgaaatgg ctaatttgcc
     8281 atggaacgaa aaaaatcaga ttaaattcag taggttgaaa taatcatcag caggcaattg
     8341 tttcaatgat agtgcgtaat tgaactgaaa cactgtatag ctaagagagt attaactgga
     8401 tcatagtcac ttaatgtgac tatgatccag gtggaaatag ggataattat tgctttttaa
     8461 cgaacaaact ggcgaagctg aacagactgg cggcgctaaa tatcagttca attcccacca
     8521 gtgtggaaac cagcgttacc gacaccatcg gcgttgcacc aaggaatatc catgcaatga
     8581 cgatatccag cacaccaata acgagctgta gccagctgcc tttcattgaa cgctgacgat
     8641 accaactcat caggcgaata acccctgcaa cacagaacaa accggcaata aatgccgcaa
     8701 tggcaaaaat gcccagctcc ggtgcgcgga tgaagaaata gccgatcaat aaataggcga
     8761 ctgcgacgag gaaaccggat aataccggcc agaaattatg actgcggttg ctgaataacc
     8821 cgacaataag cgcaatgccc gagcagatta ataatgcacc cactactgtg cttaaaatgt
     8881 cgccagagac gaacggaaaa ctgatacaca gcaacccgac gataaacagc agcacggcaa
     8941 taaactggat tgctctgcga tgttttttaa gcatctccag atcaaacttc aaaattgttg
     9001 ccttatctat atataacata gaaccaccct ataaaattaa gaagaaaatc ccctgctatc
     9061 aatctatgcc aaaaacgcg
//
LOCUS       DAPHCV010000153         9054 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.154, whole genome shotgun sequence.
ACCESSION   DAPHCV010000153 DAPHCV010000000
VERSION     DAPHCV010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.154"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..315
                     /gene="ompT"
                     /locus_tag="RH033_004171"
     CDS             <1..315
                     /gene="ompT"
                     /locus_tag="RH033_004171"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HDV2993277.1"
                     /translation="LGGTFKYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNA
                     GYYVTPNAKVYIEGAWNRVTNKKGDTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKY
                     TF"
     gene            559..1215
                     /locus_tag="RH033_004172"
     CDS             559..1215
                     /locus_tag="RH033_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDV2993278.1"
                     /translation="MKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEHLLPH
                     FLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHKISHD
                     VFDPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATILGDG
                     VVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVKGTVVMFSASGKK
                     "
     gene            complement(1270..1639)
                     /locus_tag="RH033_004173"
                     /pseudo
     CDS             complement(1270..1639)
                     /locus_tag="RH033_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            1646..2255
                     /locus_tag="RH033_004174"
                     /pseudo
     CDS             1646..2255
                     /locus_tag="RH033_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448851.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(2263..2339)
                     /locus_tag="RH033_004175"
     tRNA            complement(2263..2339)
                     /locus_tag="RH033_004175"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2303..2305),aa:Arg,seq:tct)
     gene            2610..3476
                     /gene="folD"
                     /locus_tag="RH033_004176"
     CDS             2610..3476
                     /gene="folD"
                     /locus_tag="RH033_004176"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HDV2993279.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKESAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3478..3690
                     /gene="ybcJ"
                     /locus_tag="RH033_004177"
     CDS             3478..3690
                     /gene="ybcJ"
                     /locus_tag="RH033_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HDV2993280.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3798..4319
                     /locus_tag="RH033_004178"
     CDS             3798..4319
                     /locus_tag="RH033_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDV2993281.1"
                     /translation="MPTVITHAAVPLCIALGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(4355..5740)
                     /gene="cysS"
                     /locus_tag="RH033_004179"
     CDS             complement(4355..5740)
                     /gene="cysS"
                     /locus_tag="RH033_004179"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDV2993282.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5914..6408
                     /gene="ppiB"
                     /locus_tag="RH033_004180"
     CDS             5914..6408
                     /gene="ppiB"
                     /locus_tag="RH033_004180"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HDV2993283.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            6411..7133
                     /gene="lpxH"
                     /locus_tag="RH033_004181"
     CDS             6411..7133
                     /gene="lpxH"
                     /locus_tag="RH033_004181"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HDV2993284.1"
                     /translation="MATLFIADLHLCAEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHHQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7250..7759
                     /gene="purE"
                     /locus_tag="RH033_004182"
     CDS             7250..7759
                     /gene="purE"
                     /locus_tag="RH033_004182"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDV2993285.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            7756..8823
                     /gene="purK"
                     /locus_tag="RH033_004183"
     CDS             7756..8823
                     /gene="purK"
                     /locus_tag="RH033_004183"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDV2993286.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRADETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDLNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
BASE COUNT         2204 a         2338 c         2330 g         2182 t
ORIGIN      
        1 ctaggtggca catttaaata cagcggctgg gtggaagcat ctgataacga tgaacactat
       61 gacccaggaa aaagaatcac ttatcgcagt aaagtcaaag accaaaatta ctattctgtt
      121 gcagtcaatg caggttatta cgtaacgcct aacgcaaaag tttatattga aggcgcatgg
      181 aatcgggtta cgaataaaaa aggtgatact tcactttatg atcacaatga taacacttct
      241 gactacagca aaaatggtgc aggcatagaa aactataact tcatcactac tgctggtctt
      301 aagtacacct tttaacaacg ttaaatagat tttctccccg gtggtattgt atagccccgg
      361 ggaatttgtt taccgttaaa agcaaacaat atcccctaat attgtaagca tttaacctgc
      421 gtcctggcgt gtaacctctt accgcaacac aagacccaat atattcaaat tcgttatgcc
      481 gtggcatcat ttaacccctt gtaattcatc gctataattg atttaattca caaataaaac
      541 tataacacgg tgaaactaat gaaaaaaaac acagatgatg gggctaaaat ttacacacca
      601 cttaccctaa agctttatga ctggtgggtt ttgggagtat caaatcggct tgcatgggga
      661 tgtcctacaa aggaacacct tcttccacac tttctggaac atttaggtaa caaccatctg
      721 gatattggtg ttggaactgg gttttacctt actcacgtac ctgagagtag tctgatatct
      781 ttaatggatt tgaacgaagc tagcctgaac gcggcatcga caagggctgg ggaatcaaaa
      841 attaaacata aaattagcca tgatgttttt gatccttatc ccgcggcgtt acatggtcaa
      901 tttgattcca tttccatgtt ttaccttctt cactgcctgc ctggaaatat atctacaaaa
      961 agctgtgtaa tacgcaatgc ggcgcaggcc ttaactgacg atggaactct atacggagcc
     1021 acaattcttg gtgacggcgt tgtgcacaat agcttcggtc aaaaactgat gcgcatttac
     1081 aatcagaaag gcatcttttc aaacacaaaa gattccgaag aaggcttaac acatatactc
     1141 tcagagcatt tcgagaatgt taaaaccaag gttaaaggta ctgtagtaat gttttccgct
     1201 tcagggaaaa aatagcatcc agccgcagca cgttcttgcg cacgacgtgc tgcggcataa
     1261 tcccaatgat tactctctga cagggttcac aggccactca atatcagggg cagttgatgt
     1321 atcaactcgg ttcagcaaca cctgatacat cttacaggct tccagcaacg aggtttcttc
     1381 ctccgtatcc tttatccatg atatgccgtt ccacttctga aactcccctg ccggggatac
     1441 caggtgacat tttccggtaa cggaccgagt tcagaaataa ataatgcgtc ccctgatgcc
     1501 acgtcataaa ccgttttacc ccgatggtct tcaacgagat gccacgatgc ctcatcactg
     1561 ttgaaaacaa ccacgaagcc agccggaata tctgggcaat atcagtactg tttgcaggca
     1621 aacctgtatg aggtggaatg gcacaagaga cttacaaaag aatcaggcaa aacgcctgat
     1681 tgatgagagc cccgaactac tgaaatctgt cgtcaaattt gcgctggtaa ctgttctgag
     1741 aaagtcgaac atcataaatc tggagtggca acagattgac atgcagcgac aagttgccag
     1801 ggtgaatcca gaagatagca aatcaaaccg cgccattggt gtggcgctga acgataccgc
     1861 ctctaaagtg ttgcgtgctc aaacaggcaa gcatcacaaa tgtgggtgta cataccaggg
     1921 cggctaagcg agcagatgga acatcaatgc ctgcgctcag gaagatgcgc attgacagca
     1981 agacagcatg gctatcagct tgtcgtcgtg caggagttga aaatttccgt ttctatgacc
     2041 tctggcatac ctgggcaagc tggttgattc cgtcaggcat cccattatca gtgcttcagg
     2101 aaatgggcgg atgggagtcc agagaaatgg ttcgtagata tgctcacctt gcgcctaatc
     2161 atttgactga gcacgcgagg aaaatagacg acatttttgg ttataatgtc ccactctgga
     2221 attatagaag aaataaagaa ggcgtaactg attgaatttt aatggcgcgc cctgcaggat
     2281 tcgaacctgc ggcccacgac ttagaaggtc gttgctctat ccaactgagc taagggcgca
     2341 ttgataccac ggtgtgctgt aatcgcgtga attatacggt caacccttgc tgagtcaatg
     2401 gcttttgaac cagttgctga acaagtgtac gaccgcatca gatgtagctc actatggcgg
     2461 caaaccaacg acggttatca tgataggtgt gaatcagcca ggtacagaag gttttacttt
     2521 tcagaaaacg aaaactgaca gcgcgccacg cttctgacaa aataggcgca tccccttcga
     2581 cctacgtaac agatggaatc ctctctctga tggcagcaaa gattattgac ggtaaaacga
     2641 ttgcgcagca ggtgcgctct gaagttgctc aaaaagttca ggcgcgtatt gcagccggac
     2701 tgcgggcacc aggactggcc gttgtgctgg tgggtagtaa ccctgcatcg caaatttatg
     2761 tcgcaagcaa acgcaaggct tgtgaagaag tcgggttcgt ctcccgctct tatgacctcc
     2821 cggaaaccac cagcgaagcg gagctgctgg agcttatcga tgcgctgaat gccgacaaca
     2881 ccatcgatgg cattctggtt caactgccgt taccggcggg tattgataac gtcaaagtgc
     2941 tggaacgtat tcatccggac aaagacgtgg acggtttcca tccttacaac gtcggtcgtc
     3001 tgtgccagcg cgcgccgcgt ctgcgtccct gcaccccgcg cggtatcgtc acgctgcttg
     3061 agcgttacaa cattgatacc ttcggcctca acgccgtggt gattggcgca tcgaatatcg
     3121 tcggtcgccc aatgagcatg gaactgctgc tggcaggttg caccaccacc gtgactcacc
     3181 gtttcactaa aaatctgcgt caccacgtag aaaatgccga tctactgatc gttgccgtcg
     3241 gcaagccggg ctttattcct ggtgactgga tcaaagaaag cgcaattgtg attgatgtcg
     3301 gcatcaaccg tctggaaaat ggcaaagttg tgggcgacgt cgtgtttgaa gacgcggcta
     3361 aacgcgcctc atacattacg cctgttccgg gcggcgttgg cccgatgacg gttgccacgc
     3421 tgattgaaaa cacgctacag gcgtgcgttg aatatcatga tccacagggt gagtaacatg
     3481 gcgacatttt ctttaggtaa acatccgcac gttgagctgt gcgacttgct gaaactggaa
     3541 ggctggagcg aaagcggcgc gcaggcgaaa atcgcgatag cagaaggcca ggtgaaagtc
     3601 gacggtgcgg ttgaaacgcg caaacgctgc aaaatcgttg ccgggcagac agtgagtttt
     3661 gccggtcaca gcgtacaggt tgttgcctga tccctttccc ggcctgtaac aacaggccgg
     3721 ttctcttttc agacttttgc cctctacgcc ccgttcatcc atgctgatag catcgacatt
     3781 ttgccgaagc gaccatcatg ccaaccgtta ttacgcacgc cgccgttccc ctttgcattg
     3841 ctttagggtt gggttcgaaa gtcatccctc cgcgcctgtt atttgccgga atcatcctgg
     3901 cgatgctgcc cgatgccgac gtcttgtcgt ttaaatttgg cgttgcttac ggcaatgttt
     3961 ttggtcatcg cgggtttacc cattcgctgg tgtttgcgtt tgttgtcccg ctgttatgtg
     4021 tgctgattgg acgccgatgg ttcagggctg ggctgattcg ctgctggctg tttttaaccg
     4081 tctcgttgct atcgcacagc ttgctggatt cggtaaccac tggcggtaaa ggggttggct
     4141 ggctgtggcc gtggtcagat gaacgctttt tcgccccctg gcaggtgatt aaagtcgcgc
     4201 cgtttgcatt gtctcgttac accacgccgt acgggcatca ggtgattatt tctgaattaa
     4261 tgtgggtgtg gttgccgggg atgctactga tgggaatgtt gtggtggcgc aggcgataac
     4321 cggatgcgaa aactcgcatc cggcaatagc gcaattactt acgacgccaa gtggtccctt
     4381 gcgggccatc ttccagcacg atccccatct cattcagacg gtcacgcgcc gcatctgccg
     4441 ccgcccagtc tttcgcttta cgggcatcca gacgctgttg aattaacgct tcaatctcag
     4501 ccacttcgct gtcgtctgcc tgcgcgccgc tttgcaggaa cgcttccggt tcttgctcca
     4561 gcaggcccaa tacggcggaa agtttacgca gatgagacgc catcgcgttt gccgctgcca
     4621 tatcttctgc tttcagacgg tttacttcac gcgccatatc aaacagcacg gaataggctt
     4681 ccggggtgtt gaaatcgtcg tccatcgctt caataaagcg cgcttcaaac gcttcgccac
     4741 cggcaggcgc aacggtttta tctgtgccgc gcagcgcagt gtagagacgc tccagcgccg
     4801 cacgcgcctg cttcaggttc tcttcgctgt agttcagctg gctgcgatag tggcccgaca
     4861 tcaggaagta acgcacggtt tccgcgtcgt agtatttcag cacatcgcgc acggtaaaga
     4921 agttacccag cgatttggac atcttctcgc ggtcaaccat taccatcccg gagtgcatcc
     4981 agtagttcac atactgacca tcatgcgcac aggtggactg agcgatttcg ttctcgtggt
     5041 gcgggaacat caggtctgaa ccgccgccgt gaatatcaaa gtggttaccc agctgcttgc
     5101 agttcattgc cgaacattca atgtgccagc ctggacggcc cgcgccccac ggcgacggcc
     5161 agctcggttc gccctctttc gacatcttcc acagaacgaa atccatcggg ttgcgtttgt
     5221 cgtcgaccac gtcaacgcgc gcgcctgcct gcagctggtc gagatcctga cgcgacagca
     5281 cgccatatgt cggatcggtc ggtacgtcaa acatcacgtc gccgttgtcc gccacataag
     5341 cgtgaccttt ggcgatcagt tgttcagtga gttcaataat ttctgcgata tggtgcgtcg
     5401 cgcgcggctc catatccggg cgcagaatgt tcaaagcatc aaaatctttg tgcatttcgg
     5461 caatcatgcg atccaccagc gccacaaagc tttcgccatt ttcattggcg cgtttgatga
     5521 ttttgtcgtc gatatcggta atgttgcgca catacttcag cttatagccg aggaaacgca
     5581 gatagcgcgc aaccacgtca aaagcaacaa aggtacgccc gtgaccgata tgacagagat
     5641 cgtaaacggt gattccacac acgtacatgc cgacttcccc ggcgtgaata ggcttaaatt
     5701 cctctttttg gcgtgtcaga gtattgaaga tttttagcat cgaagattcc gtttagacat
     5761 gtgtgggtaa ttgagttgcg tataataccc atatttcccg cgcgaatcag catacattgc
     5821 gtgatgatcc gatcgtgcgg ttatgctata acaccaccct atatatgacc cgaaccgggt
     5881 tgaagcacca atcaaacgga acaggatgca aaaatggtta ctttccacac caatcacggc
     5941 gatattgtca tcaaaacttt tgacgataaa gcacctgaaa cagttaaaaa cttcctggac
     6001 tactgccgcg aaggttttta caacaacacc attttccacc gtgttatcaa cggctttatg
     6061 attcagggcg gcggttttga accgggcatg aaacaaaaag ccaccaaaga accgatcaaa
     6121 aacgaagcca acaacggtct gaaaaatacc cgtggtacac tggcaatggc ccgtactcag
     6181 gcaccgcact ctgcaactgc acagttcttc atcaacgtgg ttgataacga cttcctgaac
     6241 ttctctggcg aaagcctgca aggttggggt tactgcgtgt ttgctgaagt ggttgaaggc
     6301 atggacgtgg tagacaaaat caaaggtgtc gcaaccggtc gtagcggtat gcaccaggac
     6361 gtgccaaaag aagacgttat cattgaaagc gtgaccgtta gcgagtaatc gtggcgacac
     6421 tctttattgc agatcttcat ctctgcgcgg aagaaccggc gatcaccgcc ggttttctgc
     6481 gttttttagc gggggaagcc cgcaaggccg acgcgctgta tattcttggc gatctgtttg
     6541 aagcgtggat tggcgacgac gatcccaacc cactccatca ccagatggcg gcagcgatca
     6601 aagcggtgtc cgattccggt gttccctgtt atttcattca tggcaaccgt gattttctgc
     6661 tcggcaaacg ctttgcccgt gaaagcggca tgacgttatt gccggaagaa aaggtgctcg
     6721 aactgtatgg tcgccgggta ttgattatgc atggcgacac gctgtgcacc gatgacgccg
     6781 gttatcaggc ttttcgcgcc aaagtccaca aaccctggct gcaaacgcta ttcctcgccc
     6841 tgccgttgtt tgtgcgcaaa cgcattgccg cgcgaatgcg agcgaacagc aaagaagcca
     6901 acagcagtaa atcgctggcg atcatggacg ttaaccaaaa cgcggtggtc agtgcgatgg
     6961 aaaaacatca ggtgcaatgg ctgattcacg ggcataccca tcgcccggca gtacatgaac
     7021 ttatcgccaa tcagcaacct gctttccgcg tcgtgctggg tgcctggcat actgaaggtt
     7081 caatggtgaa agtcacggca gatgacgttg agctgattca ttttccgttt taaatacccg
     7141 caactttgct gatttcacag ccacgcaacc gttttccttg ctctctttcc gtgctattct
     7201 ctgtgccctc taaagccgag agttgtgcac cacaggagtt ttaagacgca tgtcttcccg
     7261 caataatccg gcgcgtgtcg ccatcgtgat ggggtccaaa agcgactggg ctaccatgca
     7321 gttcgccgcc gaaatcttcg aaatcctgaa tgtcccacac cacgttgaag tggtttctgc
     7381 tcaccgcacc cccgataaac tgttcagctt cgccgaaagt gccgaagaga acggttatca
     7441 ggtgattatt gcgggcgcag gcggcgcagc gcacctgcca ggcatgattg ccgccaaaac
     7501 gctggtgcca gtgctgggcg tgccagtaca gagcgccgca ctgagcggtg tcgatagcct
     7561 ctactctatc gtacaaatgc cgcgcggcat tccggtgggt acactggcga ttggtaaagc
     7621 tggcgcggca aacgcggcat tactggcagc acaaattctt gcgactcatg ataaagaatt
     7681 gcaccagcgt ctgaatgact ggcgcaaagc acaaactgat gaagtgctgg aaaacccgga
     7741 cccgcgaggt gcggcatgaa acaggtttgc gtcctcggta acgggcagtt aggccgtatg
     7801 ctgcgtcagg caggcgaacc gttaggcatt gctgtctggc cggtcgggct ggacgctgaa
     7861 ccggcggcgg tgccttttca acaaagcgtg attaccgctg agatcgaacg ctggccggaa
     7921 accgcattaa cccgcgagct ggcgcgccat ccggcctttg tgaaccgcga tgtgttcccg
     7981 attattgctg accgtctgac tcagaagcag cttttcgaca agcttcacct accgaccgca
     8041 ccgtggcagt tacttgccga tcgcagcgag tggcctgcgg tgtttgatcg tttaggtgag
     8101 ctggcgattg ttaagcgtcg cactggtggc tatgacggac gcggtcaatg gcgtttacgc
     8161 gccgatgaaa ccgaacagtt accggctgaa tgctacggcg aatgtattgt cgagcagggc
     8221 ataaacttct ctggcgaagt gtcgctggtt ggcgcgcgcg gctttgatgg tagcaccgtg
     8281 ttttatccgc tgacgcataa cctgcatcag gacggtattt tgcgcaccag cgtcgctttc
     8341 ccgcaggcca acgcgcagca gcaagcgcaa gccgaagaga tgctgtcggc gattatgcag
     8401 gagctgggct atgtgggcgt gatggcgatg gagtgttttg tcaccccgca aggtctgctg
     8461 atcaacgaac tggctccgcg tgtgcataac agcggtcact ggacacaaaa cggtgccagc
     8521 atcagccagt ttgagctaca tctgcgggcg attaccgatc tgccgttacc gcaaccagta
     8581 gtgaataatc cgtcggtgat gatcaatctg attggtagcg atctgaatta tgactggctg
     8641 aaactgccgc ttgtgcatct gcactggtac gacaaagaag tccgtccggg ccgtaaagtg
     8701 gggcatttaa atttgaccga cagcgacaca tcgcgtctga ccgcgacgct ggaagcctta
     8761 atcccactgc tgccgccgga gtatgccagc ggcgtgatgt gggcgcagag caagttcagt
     8821 taattgatgc tcattttctt gccggatgcg gcgtgaacgc cttatccggc ctaccgttcc
     8881 gacaccgcct gtagacctga taagacgcgc cagcatcgca tcaggcatgg tgtaccgatt
     8941 gccggatgcg gcgtaaacgc cttatccggc ccaccgctcc aacaccgact gtaggcctga
     9001 taagacgcgc cagcatcgca tcaggcatgg tgtgccgatt gcaggatgcg gcgt
//
LOCUS       DAPHCV010000154         9048 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.155, whole genome shotgun sequence.
ACCESSION   DAPHCV010000154 DAPHCV010000000
VERSION     DAPHCV010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9048
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.155"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(102..1262)
                     /locus_tag="RH033_004184"
     CDS             complement(102..1262)
                     /locus_tag="RH033_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HDV2993287.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(1268..1939)
                     /locus_tag="RH033_004185"
     CDS             complement(1268..1939)
                     /locus_tag="RH033_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HDV2993288.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            complement(2087..3568)
                     /gene="tolC"
                     /locus_tag="RH033_004186"
     CDS             complement(2087..3568)
                     /gene="tolC"
                     /locus_tag="RH033_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HDV2993289.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNTLLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            3773..4402
                     /gene="nudF"
                     /locus_tag="RH033_004187"
     CDS             3773..4402
                     /gene="nudF"
                     /locus_tag="RH033_004187"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HDV2993290.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            4403..4825
                     /locus_tag="RH033_004188"
     CDS             4403..4825
                     /locus_tag="RH033_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HDV2993291.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            4850..5677
                     /gene="cpdA"
                     /locus_tag="RH033_004189"
     CDS             4850..5677
                     /gene="cpdA"
                     /locus_tag="RH033_004189"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HDV2993292.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            5677..6258
                     /gene="yqiA"
                     /locus_tag="RH033_004190"
     CDS             5677..6258
                     /gene="yqiA"
                     /locus_tag="RH033_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HDV2993293.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            6287..8179
                     /gene="parE"
                     /locus_tag="RH033_004191"
     CDS             6287..8179
                     /gene="parE"
                     /locus_tag="RH033_004191"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDV2993294.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(8224..>9048)
                     /locus_tag="RH033_004192"
     CDS             complement(8224..>9048)
                     /locus_tag="RH033_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2608 domain-containing protein"
                     /protein_id="HDV2993295.1"
                     /translation="LPALSYAQVKNIVTGEYSAVSKIITEKETLYPSDKILLVFDIDN
                     TLLTSGTRIGGDIWYQWQTDKLPLKPDDSQKVPCLYENTISMLYELSPMQLTEPQLPT
                     MLKQWQQKHTAFALTSRAPDTLFPTLRELKRNGIDFSSSALRKKEDTLPPFEKGKLKR
                     SWLYSQGVFFSSGQDKGVILDYMLDKMGNKYDAIVFIDDGQANIHAMTKMFSKDKWSS
                     TDFTIIHYTRVEDELIKQQGAVITTAQAEKMATDWKTLAGSLATLFPDRNKLCPIK"
BASE COUNT         2114 a         2213 c         2423 g         2298 t
ORIGIN      
        1 ttaaataaag gttggatatg tatgtgttta tatattgatg ggaattatga atggtggatt
       61 ataagagatt gccccgctta tcggggcaat attaagctgt attactcaac aaaaatatgc
      121 ggataaaacc gagacatatc ctgggtgatc aatgcacggt cttcacgaat gccaattccg
      181 gcaggttgat cgttcaccag ccagctacca atcagcatat agctgtcgcc gaatttcggt
      241 aacgggtgga attgctgaac aatcatccct tcttcgccat acggaccttc cgctgcttca
      301 atggttttgc cgttctcaat gatcgacacg tttgcgcctt cacgggagaa gatcggttta
      361 accacatatt tttccatttg cggatgatca tcttccgcaa aataagcagg cagcaggttc
      421 gggtgattcg ggaacatctc ccacagtagc ggcagaagcg ctttgttgga gataatgctc
      481 ttccacgccg gttccagcca gcgtacgcct gcatcctcca gcttggtgga gaacatctcg
      541 cgcagcataa attcccacgg atacagcttg aacaggttgg aaattacctg atcctgtaaa
      601 tccgtgaact gacctttttc gcctaaaccg atatcgtcga tgtagaggaa ttcggtagcg
      661 atttcagctt ccgtcgcgca gtcctgcaaa tactgaatgg ttccgcgatc ttccaccgtg
      721 tcgcgacagc aggtgagatg cagcaactgg aagccatact gttcacgcaa ctcgacaaag
      781 cgatcgatta gcttttcttg cagactgtta aactggtcgc tgccctccgg caagttaccg
      841 gcgttaagct gatcttccag ccagatccac tgaaagaacg ccgcctcgta tagtgacgtt
      901 ggtgtatcgg cgttattttc cagcagttta ggttcgccag tgccatccca cgccagatca
      961 agacgcgaat aaagcgatgg ctggtgagtc agccatgact ggcgcacaaa actccaggtg
     1021 tgttttggta tgcggaattt ggtcatcagc tcatcgctgg cgatcacttt ttccaccact
     1081 ttcaggcaca tctggtgcag ttcggcggtg acttcttcca gcttttcaac ctgggcgagg
     1141 gtcaacttgt agtaagcatc ttcacaccag tacggctcgc cgtacatggt gtgaaaattg
     1201 aaaccgtatt cgtgggcttt ttcgcgccag tccgggcgct cggtaatact gactctttcc
     1261 atcggtatca gccacccatt gaacgagaag aggtgccggt tgcgctacgc tgcatagtgc
     1321 tttgtttggc aacagattca ccaaaaccgc cacgggtaac agtagtagtg gtcgccggtt
     1381 ttggtgccat tgccgtcttc ggtacggtca tggtgcggcc tggctgggct gcgccatagt
     1441 ttttaccggt cgcgtcggta tatttaccgt aagccgggct ggccgggttt ttcgaggaga
     1501 acagcggctg ctgtgcaaat cccgcgccgc cgcccatcag acgtcccatc atgtaaccgg
     1561 ccatcagcgg catccagaaa ctcccgctgg attgctgggc ctgcgcctgg ttttctggtg
     1621 ccatgccagc ctgggctggt gcctgctggc actgaccttc accaaattca gcaacacagt
     1681 cttcacgggt ggcgtatttc ggcgcagtac gttcggcttc tttcagcgca ttgttgtacg
     1741 cggtggtaca ttcggcgctt ttgcctgggt ttgcagctga acagtcgtca gcattttgat
     1801 agagagacac tgtttcatca ctcttttcac agccagccag cataaaaaca gtggcaaccg
     1861 cgagagcgac tggtgtcaga tggcgtgcgc tccagttttt gcggaacgat gcgtggcgta
     1921 tggattttgt ccgtttcatt tttgtcttcc gggaccagtg gtaaataccc atcagaatag
     1981 aggatggctg gtcgaaattg aagcgagaag ggggaagaat gcggcagata acccgtatct
     2041 ttacgttgcc ttacgttcag acggggccga agccccgtcg tcgtcatcag ttacggaaag
     2101 ggttatgacc gttactggtg gtagtgcgtg cggatgtttg ctgaacgacg ggtgccgggc
     2161 tatcaggcgc ataaccatca gcaatagcat tctgttccgg cgtttgcggg gcaacgtttt
     2221 ccggattagt ggaaaccggt ttgctcagcg cattgttcag tgccagaaga tcctgctcgt
     2281 tcaacgtacc cagagctgat ttaatattca actggttaat caggtagtta taacgcgcat
     2341 tcgccagctc ttgcttggcg ttgtacagcg tggtggtcgc atccaacaca tcaacaatgg
     2401 tacgcgtacc gaccgagtaa cccgcttcca tcgcgtctaa tgagctttga gcggaaacta
     2461 cggcttgttt gtaggcgtta atgctactga tagaagcatt aatgttgttg aaggaggaac
     2521 gtacggtttg cacgacgcta cgatgcgcgc tttccagttg ctcgctggca ccaacaaagt
     2581 tgtactgggc ctgtttcacc tgcgagttaa ccattccgcc ctgataaatc ggcagcgaga
     2641 agctcaggcc aactttgttc tggcccatat tgctatcgtc atactgggta ccagcggcac
     2701 cacgggtttt cgaaccgcta taagaggtgt cagaaatccc ggtagaagcc gttaaatcca
     2761 gagtcggtaa gtgaccatcc tgcgcctggc gaatttgctc gcgcgccagg tcctggctca
     2821 agcgcgcctg taacagcgac aggttgcgtt tttcggcttc tttcagcagc gtgttaaccg
     2881 gctgtggttt gtcggtttta aagttttcga cattcagcgc cgccagttct ggatagtagt
     2941 taccggtgat ctggcgcagc tgctctaccg cgttatcaag gttattacgt gcggtcactt
     3001 cgttcgccag cacggtatcg tactgtgcgc gggcgttctg cacgtcagtg atcgctacca
     3061 ggcccacgtt aaaacgttgg gtggtttgat ctaattgacg gtagatcgct tctttttgcg
     3121 cctgtgtata ggaaagaacg tcaatagcat tcaacacgtt gaaataagcg gtcgcggtgt
     3181 tgaggatcaa ggtttgttga tcggtctgat acgtgacgtc ctgaatccca gctgcttttt
     3241 cctgcagcgt taacgcacgc catttcgaca tatcaaaaat ggattgagtt aactgcaggg
     3301 acgcactggt cgcgttagag ttgatgccgt tcgcgtcgcg gtagccgttg ctataggtgt
     3361 aatctgcacc taaacctagc tgtggcagta atggactgcg cgcttcatta attttttcaa
     3421 aggcagcatc acgatcggcg gcagacttac gcaattccgg gttactaagg cgtgcttgct
     3481 gataaacttg catcaggttc tcggcctggc tcaacgaact gaaaccagaa aggctcaggc
     3541 cgataagaat ggggagcaat ttcttcattt gcattccttg tggtgaagca gtatttagcg
     3601 cgatcaaact gtaaaattat tgcggattct agcagaagcc gctaccgcaa aaggttggcg
     3661 ttacgtgcca tagggcgtta atgtgcctca atttaacaca tgactgctca aacggcagtc
     3721 aaacgtcgct gaaattcaca tttaattcac tattagtgcc aggacattaa caatgcttaa
     3781 gccagacaac ctgcccgtta catttggcaa aaacgatgta gaaattattg cacgagaaac
     3841 actttatcgc ggcttttttt cattagatct ttatagattt cgtcatcgtc tattcaacgg
     3901 gcaaatgagt catgaggtac ggcgggaaat ttttgagcgc ggtcacgccg cagtcttgct
     3961 accctttgac ccagtgcgtg atgaagttgt gctgattgag cagattcgaa ttgccgcgta
     4021 cgacactagc gaaaccccct ggctactgga gatggttgcc gggatgattg aagagggtga
     4081 aagtgtggaa gatgttgccc gtcgcgaagc gattgaagag gcggggctga tagtcaaacg
     4141 gaccaaaccg gtgttaagtt tcctggcaag cccggggggc accagtgagc gttcgtcaat
     4201 tatggtgggc gaagtggacg ccacgaccgc aagcggtatt cacggtctgg ctgatgaaaa
     4261 cgaagatatt cgcgttcatg tggtaagccg ggaacaggca taccagtggg tagaagaggg
     4321 gaaaatcgac aacgcagcgt cggtcatcgc tttgcaatgg ctgcagctgc atcatcaagc
     4381 gttaaaaaat gagtgggcat aaatgaagcg ttacacacct gactttcctg aaatgatgcg
     4441 cctgtgcgag atgaactttt cacaattgcg ccgtttgtta ccgcgcaatg acgcacccgg
     4501 cgaaactgta agctatcagg tggcaaacgc acaatatcgg ctgacgattg tggaatcgac
     4561 ccgatacact accctggtga caatagaaca gactgcgccc gcgatcagtt actggagcct
     4621 tccgtcaatg acggtgcgtc tgtatcatga cgcgatggtg gctgaagtgt gttcaagcca
     4681 gcagattttt cgcttcaaag cgcggtatga ttatcctaat aaaaagttgc atcaacgcga
     4741 cgaaaagcat caaattaatc agtttttagc ggactggttg cgatactgtt tagcacatgg
     4801 agcgatggcg attccggttt attagcgtcg tgaaacctaa ggacaccatt tggaaagcct
     4861 gttaaccctt cctctggctg gtgaggccag agtcaggatt ttacaaatta ccgacactca
     4921 cctgtttgcg caaaagcacg aagccctgtt aggggtaaac acctgggaaa gctaccaggc
     4981 ggtgctggag gcgattcggc cacaccagca cgaattcgac ctgattgtcg cgacaggtga
     5041 tttagcgcag gatcaatcct ctgcggccta tcagcatttc gctgaaggca tcgcaagttt
     5101 tcgtgcgccc tgcgtctggc tgccgggcaa ccacgatttc cagcccgcga tgtacagcgc
     5161 gttacaggat gcgggtatct ccccggcgaa gcgggtgttt attggtgagc aatggcaaat
     5221 cctgttgctg gatagccagg tgtttggcgt gccgcacggt gagctgagcg agtttcagct
     5281 tgagtggctg gaacgtaaac tggccgatgc gccagaacgc catacgttgc tgctgctgca
     5341 tcatcatccg ctacctgcgg gttgtagttg gcttgatcaa cacagcctgc gtaacgcggg
     5401 cgaactggat accgtgctgg cgaagtttcc gcacgtcaaa tacttgctgt gcggtcatat
     5461 tcatcaggag ctggatctcg actggaacgg tcgccgcctg ctggcaacgc cgtcgacctg
     5521 tgtgcagttt aagccgcact gttccaactt tacgctggac accatcgcgc ccggctggcg
     5581 tactctcgag ttacatgctg atggcacgct gaccaccgag gtgcatcgcc tggcggacac
     5641 acgtttccaa cctgataccg cttcagaagg ctactgatgt ctacgcttct ttatttacac
     5701 ggtttcaaca gctcgccgcg ctctgcaaaa gcgagcttgt taaaaaactg gcttgcggaa
     5761 catcaccctg acgttgagat gatcatcccg cagttgccgc cgtatccttc cgatgcggca
     5821 gaactgctgg aatccattgt cctggaacat ggtggcgatt cgctgggtat tgtcggttcg
     5881 tcactggggg gatattacgc cacctggttg tcgcaatgtt ttatgctgcc cgcagtggtg
     5941 gtcaacccgg cggtgcgccc gtttgaactg ctgacggact atctcggtca gaacgagaac
     6001 ccctacaccg ggcagcaata tgtgctagag tcacgccata tttacgatct caaagtcatg
     6061 cagattgacc cgctggaagc gccggatttg atctggctgc tgcaacagac gggagatgaa
     6121 gtgctggatt accgccaggc ggtggcgtac tacgcttcct gccgccagac tgtcatagaa
     6181 ggcggcaacc acgcattcac gggcttcgaa gattatttca acccgatcgt cgattttctt
     6241 ggtctgcacc atctctgacg atcaactcga attgctaact taaaccatga cgcaaactta
     6301 taacgctgat gccattgagg tactcactgg gcttgagccg gttcgccgcc gtccggggat
     6361 gtataccgat accactcgcc ctaaccattt ggggcaagaa gttattgata acagtgtcga
     6421 tgaagcactg gcgggtcacg caaaacgcgt ggacgtaatc ttacatgctg accagtcgtt
     6481 agaagtgatt gacgatgggc gcgggatgcc ggtggatatt cacccggaag agggggtacc
     6541 ggcggttgaa ctgattcttt gccgtctgca tgcaggcggt aaattctcta acaaaaatta
     6601 ccagttctct ggcggcctgc atggcgtggg gatttcggtg gttaacgccc tttcgaagcg
     6661 cgtagaagtt aacgtacgcc gcgatggtca ggtttataac atcgcctttg aaaatggcga
     6721 aaaggtgcag gatttacagg ttgtcggcac ttgcggtaag cgcaataccg gtaccagcgt
     6781 gcacttctgg ccggatgaaa ccttctttga cagcccgcgt ttttctgttt cacgcctgac
     6841 gcatgtgctg aaggccaaag cagtactgtg ccctggtgtt gagatcacct ttaaagatga
     6901 gatcaacaac accgaacagc gctggtgcta tcaggacggt ctgaatgatt acctggcgga
     6961 agcggtaaac ggtttaccga cgctgccaga aaaaccgttt atcggtaatt tcgctggcga
     7021 tactgaagcg gtggactggg cgctactgtg gctaccggaa ggcggtgaac tgctgaccga
     7081 aagctacgtc aacctgatcc caacgatgca gggcggtacc catgttaatg gcctgcgtca
     7141 gggcctgctg gacgcgatgc gtgagttctg tgaataccgc aacattctgc cgcgcggtgt
     7201 aaagctgtcg gcggaagata tctgggatcg ctgcgcctat gtgctgtcag taaaaatgca
     7261 ggatccgcag tttgccgggc agaccaaaga acgtctctct tcgcgtcagt gtgcggcatt
     7321 cgtttcgggc gtggtgaaag atgccttcat cctgtggctg aaccagaacg ttcaggcggc
     7381 ggagctgttg gcagagatgg cgatttccag cgcccagcgt cgtatgcgtg cggctaaaaa
     7441 agtggtgcgt aaaaagctaa ccagcggccc ggcgctgcct ggtaaattgg ctgactgtac
     7501 cgcgcaggat cttaaccgta ccgaactatt ccttgtggaa ggtgactccg caggcggatc
     7561 tgccaagcag gcgcgcgatc gcgaatatca ggcgatcatg ccactgaaag gtaagatcct
     7621 taacacctgg gaagtctctt ccgacgaagt gctggcttcg caggaagtgc acgatatttc
     7681 ggtagctatc ggtatcgatc ctgacagcga cgatttgagc cagcttcgtt acggcaagat
     7741 ctgtatcctg gcggatgctg actccgatgg tctgcacatt gccacgctgc tctgcgcttt
     7801 gtttgtaaaa cacttccgcg cattggtgaa acacggtcac gtttacgttg cactgccacc
     7861 gctctaccgt attgacctcg ggaaagaggt ttattacgcg ctgacggaag aagagaaaga
     7921 gggcgtactt gagcaattaa aacgcaagaa aggcaagcca aacgttcagc gttttaaagg
     7981 tctcggggaa atgaacccga tgcaattgcg cgaaaccacg cttgatccga acactcgccg
     8041 tctggtgcag ttgactatcg atgatgaaga cgatcagcgt actgacgcga tgatggatat
     8101 gctgctggcg aagaaacgct cggaagatcg ccgcaactgg ttgcaagaga aaggcgacat
     8161 ggcagagatt gaggtctgat aatcttaacc cggcaaacat gtgttttgcc gggttgtcgt
     8221 tgattatttt attgggcaca gtttattacg atctggaaat agtgttgcca gcgaaccagc
     8281 cagagttttc cagtccgtag ccattttttc tgcttgtgca gttgttatta ctgcaccttg
     8341 ttgtttaata agctcatcct ccactctggt atagtgaata atggtaaaat ccgtagagga
     8401 ccatttatcc ttagagaaca tcttcgtcat cgcatgaatg tttgcttgcc cgtcatcgat
     8461 aaaaacaatc gcgtcatatt tgttccccat tttatccagc atataatcga gaattacgcc
     8521 tttatcttgc ccggaagaaa aaaatacccc ctggctgtac agccatgacc gtttgagttt
     8581 ccctttttca aagggtggca aagtatcctc tttcttcctg agtgcggaag acgaaaaatc
     8641 aatgccgttt cgctttaatt cccgcaatgt cggaaagaga gtatccggtg cacgggatgt
     8701 cagagcaaaa gcggtatgct tttgttgcca ctgttttaac attgtcggta actgtggctc
     8761 ggtaagctgc atagggctaa gttcgtaaag catactgatt gtattttcat acaggcaagg
     8821 aactttttgc gagtcatccg gctttaaagg caatttatcg gtttgccatt gataccagat
     8881 atcaccgcca atgcgagtgc cactcgttaa taacgtatta tcaatgtcaa aaaccaataa
     8941 tatcttatcg gatggataga gagtttcctt ttctgtgata attttactga ccgcactata
     9001 ttcgccagta actatatttt ttacctgtgc gtaagataaa gcaggcag
//
LOCUS       DAPHCV010000155         8863 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.156, whole genome shotgun sequence.
ACCESSION   DAPHCV010000155 DAPHCV010000000
VERSION     DAPHCV010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.156"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(557..1087)
                     /locus_tag="RH033_004193"
     CDS             complement(557..1087)
                     /locus_tag="RH033_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDV2993296.1"
                     /translation="MKLTEESLNLVIDILNDLICEGKMLNDVERRSLLNAVMAITAVK
                     ERSVVSVSSRKEQSKKEKKEREIDPRFPNAGVAWNEADESLLRDVLEDVPDDEIGKHL
                     FWLAEKLGRTPYSVACKVRQIKKLPAEWKDQFRKISDRIRSSGMSVSEYLQRQGLLSP
                     ESGEENNPVSHNNNVA"
     gene            1757..2488
                     /locus_tag="RH033_004194"
     CDS             1757..2488
                     /locus_tag="RH033_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HDV2993297.1"
                     /translation="MNQPIHNDYWLSRFESILNSALVQHRAVSLIRVDLRFPEHMPVT
                     IMDPDPDSAVISRFFESLKAKIQAYQRKKRRTNKRVRATTLHYFWCREFGKEKGRKHY
                     HVILLLNKDTWCSPGDFTVPSSLATLIQLAWCSALHLEPWQGNGLVHFSRRTPFRKPA
                     SSDARPSSDDTPLSGGCSETRKASDKKPGEAAVLWIKRGDVEAMQKAMERARYLVKYE
                     TKQHDGSGQRNYGCSRGAGRLLDGR"
     gene            complement(2599..3827)
                     /locus_tag="RH033_004195"
     CDS             complement(join(2599..3512,3512..3827))
                     /locus_tag="RH033_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDV2993298.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRGKKVDSTRALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWKDGRRSRHTDDTDV
                     LLRIHHVIGELPTYGYRRVWVLLRRQAELDGMPAINAKRVYRIMRQNALLLERKPAVP
                     PSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGFN
                     SETVQDVMLGAVERRFGNDLPSSSVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRS
                     PESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREY
                     LRQRACNGLSDNRCLEI"
     gene            4020..6068
                     /gene="ireA"
                     /locus_tag="RH033_004196"
     CDS             4020..6068
                     /gene="ireA"
                     /locus_tag="RH033_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor IreA"
                     /protein_id="HDV2993299.1"
                     /translation="MKNKYIIAPGIAVMCSAVISSGYASSDKKEDTLVVTASGFTQQL
                     RNAPASVSVITSEQLQKKPVSDLVDAVKDVEGISITGGNEKPDISIRGLSGDYTLILV
                     DGRRQSGRESRPNGSGGFEAGFIPPVEAIERIEVIRGPMSSLYGSDAIGGVINIITKP
                     VNNQTWDGVLGLGGIIQEHGKFGNSTTNDFYLSGPLIKDKLGLQLYGGMNYRKEDSIS
                     QGTPAKDNKNITATLQFTPTESQKFVFEYGKNNQVHTLTPGESLDAWTMRGNLKQPNS
                     KRETHNSRSHWVAAWNAQGEILHPEIAVYQEKVIREIKSGKKDKYNHWDLNYESRKPE
                     ITNTIIDAKVTAFLPENVLTIGGQFQHAELRDDSATGKKTTETQSVSIKQKAVFIENE
                     YAATDSLALTGGLRLDNHEIYGSYWNPRLYAVYNLTDNLTLKGGIAKAFRAPSIREVS
                     PGFGTLTQGGASIMYGNRDLKPETSVTEEIGIIYSNDSGFSASATLFNTDFKNKLTSY
                     DIGTKDPVTGLNTFIYDNVGEANIRGVELATQIPVYDKWHVSANYTFTDSRRKSDDES
                     LNGKSLKGEPLERTPRHAANAKLEWDYTQDITFYSSLNYTGKQIWAAQRNGAKVPRVR
                     NGFTSMDIGLNYQILPDTLINFAVLNVTDRKSEDIDTIDGNWQVDEGRRYWANVRVSF
                     "
     gene            complement(6248..7412)
                     /locus_tag="RH033_004197"
                     /pseudo
     CDS             complement(6248..7412)
                     /locus_tag="RH033_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            7450..8662
                     /locus_tag="RH033_004198"
                     /pseudo
     CDS             7450..8662
                     /locus_tag="RH033_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    7731..7847
                     /locus_tag="RH033_004198"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT         2312 a         2015 c         2111 g         2425 t
ORIGIN      
        1 cacttctaca agtgttttcg ttaagcattg ctatacacat ccagtttagt aaatctttac
       61 gttaactaaa tcagtttaat gctgctgtaa aacctttctg tttaagcaat caatctgcta
      121 ttcgatttta ctgatgttac gtttaacctt tccttagatt tcgagtttcg acgttagctt
      181 gagaaaagta tctttacgta aagcccagtg aattaactca aatccgattg aggtaacctg
      241 atgcgattta ctgtgagttt gcgataacac tgttaaatgg cttatggatg attttgtaac
      301 ggcgagattg ataaggtgat taactggccc tggtgttttt tctcagcgta aggtgattat
      361 cttcatcata agggtctttt cagaaaaagt gtgatgattg gttgtggtgt tttgattcag
      421 atgaggatct gccaatgatt atgatgtatt gtaagaaata acgccttcag aatgttggag
      481 catgcactta cacaaactgt ttttctaaaa ttaagggaac agtttaaaga gtataaactg
      541 ttcatcttct tcaggattaa gctacattgt tgttatgact gacaggattg ttttcttccc
      601 ctgactctgg gcttaacagt ccctggcgtt gtagatattc actgactgac attccagaac
      661 tacggatcct gtcagagatt ttacgaaatt gatccttcca ttctgcaggt aatttcttaa
      721 tttgtctgac tttacaggct acactgtacg gggttcgccc tagtttctct gccagccaga
      781 aaaggtgttt accaatttca tcgtccggta catcctccag aacatctcgc aataaactct
      841 cgtctgcctc attccatgcc acaccagcat ttgggaaacg aggatcgatc tctctttcct
      901 tcttctcctt cttactttgc tcttttcggg atgatacaga tacgacagaa cgttctttta
      961 ccgctgtaat tgccataact gcattgagca gagatctgcg ctccacatcg ttcagcattt
     1021 ttccttcaca gatcaaatca ttcaggatgt caatgactag attcagactt tcttctgtta
     1081 gcttcatatt tcagaccttg aagtatgtag ataatcagca caattactaa tgtgataaat
     1141 atcagaagat aatttacagg taaaccggaa aatacatctg aagaataaag gcctcagctt
     1201 aacgtttcag ccagtttgtg agctgattga ggtacggcga tgacattaac gggaattact
     1261 cccctatagc tctgagctta tttttcaccc tggcaacata tggtggctac tgcgcatggt
     1321 tttggagtag atatcttact actcgtagaa ttgtgcttac tggtcaggcc agcgcacagg
     1381 cattccgtgc aatcaataga acactggttt tttagtcttc cgttacccat caggatgtta
     1441 gtgcagattc cggtgtattc gatcagttgt tcggcgaatc agcgatcgat cacgatgcga
     1501 tttcgtatgt tagggatgct ggtatgatta ctcgctgaaa aataatgtga aaaggcagtt
     1561 tttctttaga catttagctc attcatgctg ttgttttacg ttttgctgtc gtgtgcagga
     1621 ttatcttttc gttacgggac gattcattcc gttttaatca ggagctattg gcgttgctca
     1681 ttggtgggat gccgtaaagt tttaccgcgg cgattaatga tgtgaagtca atccaaatca
     1741 acggagatct ctcatcatga atcaaccaat acacaatgat tactggttat cccgttttga
     1801 aagtattctc aacagtgccc tggtgcaaca ccgtgccgtc tcgttaatcc gggtggattt
     1861 acgtttccct gagcatatgc ctgtcaccat catggatccc gatccggatt cagcggtgat
     1921 ttctcgtttt ttcgaatccc tgaaagccaa aattcaggct taccagcgga aaaaacgacg
     1981 taccaacaag cgtgtgcgtg caaccaccct gcattatttc tggtgtcgag agtttggcaa
     2041 ggaaaaaggc aggaaacatt atcacgtgat attactgctc aacaaagata cctggtgctc
     2101 gccaggggat ttcaccgttc cttcttcgct ggcgacgctg atccaactgg catggtgtag
     2161 cgctctgcat cttgagccct ggcagggtaa tggactggtt catttttcca ggcggacgcc
     2221 tttccgtaaa ccggcatcat ctgatgctcg cccttcttcc gatgatacgc ctttgtcggg
     2281 tggatgttct gaaaccagga aggcttcaga caaaaagccg ggtgaagccg ctgttctctg
     2341 gatcaagcgt ggtgatgtgg aagcgatgca gaaagccatg gagagagccc gttatctcgt
     2401 gaagtatgag acgaagcagc atgacggttc tggtcaacgt aattatggtt gcagccgtgg
     2461 agcggggcgt ctactggatg gcaggtgaac cctgtaaaac ggcatccggt gccagagtat
     2521 atgtcacagt aaggtcgttg gctgacgccc ttagctcgtt ttctgaaaaa gtttttctga
     2581 agtaatgtgg atttgcccct atatttccag acatctgtta tcacttaacc cattacaagc
     2641 ccgctgccgc agatattccc gtggcgagcg ataacccagc gcactatgcg gatgccattc
     2701 gttataatgc tcgaacgcct ctgcaaggtt ctttgctgcc gttaacccgt ctggtttggg
     2761 catgatactg atgtagtcac gctttatcgt tttcacgaag ctctctgcga ttccgttact
     2821 ctccggactc cgcaccgccg tgttcttcgg ttcaagtccc aacatccggg cgaactggcg
     2881 tgtttcatta gcccggtagc atgaaccatt atccgtcagc cactccactg aagacgacgg
     2941 aagatcgttg ccgaagcggc gttccaccgc tcccagcatg acgtcctgta ctgtttcact
     3001 gttgaagccg ccggtagtga ccgcccagtg cagtgcctca cgatcacagc agtccagcgc
     3061 gaacgtgaca cgcagtctct ctccgttatc acagcagaac tcgaacccgt cagagcacca
     3121 tcgctgattg ctttctttca cggccactct gcctgtatgt gcccgtttcg atggcggtac
     3181 agcaggtttt cgctcaagca acagcgcatt ctggcgcatg atccggtaaa cacgtttggc
     3241 attgatcgca ggcataccat caagttcggc ctgtctgcga agcagcaccc atacccgacg
     3301 ataaccatat gtgggcagct ctccgataac atggtgtata cggagaagca catccgtatc
     3361 atcagtgtga cggctgcggc gaccatcttt ccagtcatcg gttcgtctga gaatgacgtg
     3421 caactgcgca cgcgacaccc ggagacaacg gctgactaag cttactcccc atccccgggc
     3481 aataagggcg cgtgtgctat ccactttttt gcacgcccat attcaacggc ttctttaagg
     3541 agttcatttt ccatcgtttt tttgccgagc aggcgctgga gttctttaat ctgcttcatg
     3601 gcagcagcaa gttcagaggc aggaacgacc tgttctcctg cggccacagc agtaagactt
     3661 ccctcctggt attgcttgcg ccagagaaat aactggctgg ctgccacacc gtgttgccgg
     3721 gcaacaaggg agaccgtcat tcccggttca aaactctgct gaacaatagc gatcttttcc
     3781 tgtgtagtac gccgtctgcg tttctccggt cctaagacat caatcatctg ctctccaatg
     3841 actagtctaa aaactagtat taagactatc acttaaataa gtgatattgg ttgtctggag
     3901 attcaggggg ccagtctact gaatgagcga aatgtgattt accttctttt ctgttcattg
     3961 taaattccct ctttgctaac gcaaatcatt atcattacgc ctttggcaaa gggaaattta
     4021 tgaagaacaa atatatcatt gctccgggca ttgccgtgat gtgttctgca gttatatcat
     4081 caggttatgc cagttctgat aaaaaagaag atacgcttgt tgttactgcc tccgggttca
     4141 ctcagcagct cagaaatgcc ccggccagtg tctcagtcat tacttcagaa caactgcaaa
     4201 aaaaaccggt ttcagatctg gtcgatgcag taaaagatgt tgaagggatt agtatcactg
     4261 gtgggaatga aaaaccggat atcagtatac gtggtctaag tggcgattac acgctgattc
     4321 tggtcgatgg acgacgtcag agcggtcggg aatccagacc aaacggcagc ggcggttttg
     4381 aagccggatt tatccctcct gtggaagcaa ttgaacgcat tgaagtgatc cgtggcccta
     4441 tgtcttccct gtatggttct gatgccatcg gaggggtcat taatatcata accaaaccag
     4501 ttaataacca aacatgggat ggcgtacttg gacttggggg gattattcag gaacatggga
     4561 aatttggtaa ctcaaccaca aatgacttct atctgtcagg cccattgatt aaggataaac
     4621 ttggtcttca gctatatgga ggaatgaact atcgcaagga agatagtatc tctcagggaa
     4681 caccggcaaa agataataag aatataacgg caacgctcca gtttactccg actgaaagcc
     4741 agaagtttgt ttttgaatat ggaaaaaata accaggtgca tacattaaca cctggtgagt
     4801 ctctcgatgc ctggactatg cggggaaatc ttaaacaacc aaacagtaaa agagaaacgc
     4861 ataattcacg tagtcactgg gtagcagcat ggaatgccca gggcgaaata ctgcatcctg
     4921 aaattgctgt ttatcaggag aaagttattc gtgagattaa atcaggtaaa aaagataaat
     4981 ataatcattg ggatcttaat tacgagtcaa gaaaaccgga aataaccaac acaatcatag
     5041 atgcaaaagt gacggcattt ctgccggaaa atgtactgac catcggaggt caatttcagc
     5101 atgcagagct ccgtgatgac tcagccacgg gtaaaaaaac gacagaaaca cagtctgttt
     5161 caattaaaca gaaagctgtt tttatagaaa atgaatatgc agcaacggat tctctcgccc
     5221 tgactggagg actgcgtctc gataatcatg aaatctatgg cagttactgg aatccaagat
     5281 tgtacgctgt ttataacctg accgataatc tcacactcaa aggggggatc gcaaaagcat
     5341 ttcgggctcc ttcaattcgt gaggtgagtc ctggatttgg aacactgacg cagggtggtg
     5401 cctctattat gtatggaaac agggacctga aaccggagac cagtgtaacc gaagagatcg
     5461 gtattattta tagtaatgat agtggttttt cggcgagcgc gacgctgttt aatactgatt
     5521 ttaaaaataa gttgaccagt tacgatatag gtacaaaaga tccagtcacc gggttaaaca
     5581 cttttattta tgataatgta ggtgaggcaa atatcagagg ggtggagctt gcaactcaga
     5641 ttcctgtgta tgataaatgg catgtatctg caaactatac atttactgac tctcgtcgaa
     5701 aaagtgatga cgaaagtctc aatggcaagt cgctgaaagg ggaacctctg gaaagaactc
     5761 ccagacatgc agccaatgca aaactggaat gggattacac tcaggatatt acattttatt
     5821 catctctgaa ttatacggga aaacaaatct gggcagcaca aagaaatggt gctaaggttc
     5881 cccgcgttcg taatggattc acatctatgg atattggtct aaattaccag attctgccag
     5941 acacgctgat taattttgcc gttcttaacg tcacagacag aaagagcgag gatatcgata
     6001 ccattgatgg taactggcag gtcgatgaag gacgccgtta ttgggctaat gtaagagtat
     6061 ccttctgaga gtatataaag acaacctgag gccgtgagcc tcaggtacca gtgtagtaat
     6121 cagtaatgtc agtttccagg ttgtaatgct tttaatcttg attaaaataa tcttaataat
     6181 ttaaacttgt taaatatcga tgattttaat ttgagagcgt tcagaattct gtgtcacgtt
     6241 aaaaattcta cagcgtcatc acattattca gccgcccttc aaaataaatg gctaactgcg
     6301 acaatgtcaa attccagctt tggatgggca ttgtccattt ctcctgtgcg ttcatcagtc
     6361 ccagataaag tagcttcaac aggctgtttt cattcgggaa agcaccttta ggttttggtc
     6421 agtttcctga actggcggtg gactgattct atcgcgttgg ttgtgtagat caccttgcgg
     6481 atattcgccg gatagcggaa gtacgctgac agattttccc tcttgcggcg ccaggactgg
     6541 agcaccaccg gatattgctg cccccgtttc gcttccagct catccagcgc cgtttccgac
     6601 accgcacgat acactggctt caggtcggcc atgaacgctt tatggtgttt tgacgccaca
     6661 tatttaatcg agttacgtat ctggtgaatg acgcacaact gtacctctgt ctgcggataa
     6721 atgctgttta tcgcttcagg gaagccggtc agcccatcca cgcaggcaat cagaatgtct
     6781 tcaacgccac ggttttgcag atcacttaat actgacagcc agaagttagc gccttcgctt
     6841 tccgacaaat acaggcccag gacttctttt ttgccttcca gattcagcgc cagtacggta
     6901 taaactgcct tgctctgata acacccatct tcgcggattt tatagtgaat ggcgtccagc
     6961 cagacgaagg gataaacctt ttccagcggg cgctgctgcc actgttttaa ctcaggaatg
     7021 actttatcgg taaccgcact gatggtagcc gtggaaacac tgaaggcata taaatcttcg
     7081 atctcccggc tgatatcctg gtagctcatc cccagcgcga acaggcgaat gatcttgcgc
     7141 tcgagctcat cagacagcgt ggtctggtgc ttcttcacca gttggggttc aaaataacca
     7201 ttacgatcac gtggagttgc cagctcaaag ctgccagtcg gggctttaat ggtctttttt
     7261 ccggaaccat ttttacgatt gacttcaaaa tcctgagcca gatgggagtc gagttcagcg
     7321 gccagagcgg tttctgttaa ctgtttgatt aatggcgtta ggataccatc cttgccggtt
     7381 aatgcttggc cggactgaac cgccccggaa atcctggaga ctaaactccc tgagaaagag
     7441 gtaaacagga tgactaaaaa tactcgtttt tcccccgaag tccgtcaacg ggcagttcgt
     7501 atggttctgg aaagtcaggg cgaatatgac tcacaatggg cggcaatttg ttccattgct
     7561 ccaaagactg gctgtacgcc ggagactctg cgtgtctggg ttcgccagta tgagcgggat
     7621 accgggggcg gagatggagg gctcaccagt gctgaacgtc agcgtctgaa agagctggaa
     7681 cgtgaaaatc gtgaactgcg ccgcagcaac aatatccttc gccaggcttc cgcttatttt
     7741 gcgaaggcgg agttcgaccg cctctggaaa aagtgatggc actgctggat aagctgcgtg
     7801 agcagtacgg ggtcagaccg gtatgcagtg aactgcatat tgccccgtca acgtattacc
     7861 actgtcagca acagcgacat catcctgata aacgcagtgc acatgcacag cgcgatgact
     7921 ggctgaagag agagatacag cgcgtatacg atgaaaatca tcagtgtacc gtgtgcgtaa
     7981 agtctggcgc cagttgttac gggaaggaat caggatggcc agatgtacag tggtgcgcct
     8041 catggcggtt atgggacttg ccggtgttct ccggggtaaa aaggtccgca ctaccgtcag
     8101 ccggaaaacc gttgccgcag gtgaccgcgt aaaccgtcag ttcgtggcag aacgtcctga
     8161 ccagctgtgg gtgctgattt tacttacgtc agcacatggc agggcttcgt ctatagtggc
     8221 gttcatcatt gatgtatttg ccggatatat cgtggggtgg tgggtctcat cgtctatgga
     8281 aacgacattc gtgctggatg cgctggagca ggcgttgtgg gcccgccgtc cgtccggcac
     8341 catccatcac agcgataaag gctctcagta tgtgtcactg gcctatatgg agcgattaaa
     8401 agaagcaaaa ctgctggcat caacagggag tacaggcgac tcgtatgaca acgcgatggc
     8461 ggagagcatc aatggtcttt acaaagcgga ggtaatacac cgtaagagct ggaaaaaccg
     8521 tgcagaagtg gaactggcca cactcacgtg ggtggactgg tataacaatc gacgattgct
     8581 gggaaggctg ggccatactc ctccggcaga agcagaaaaa gcttattatg cttccatcgg
     8641 aaacaatgat ctggcagcct gagttcacag ataaaacact ctccaggaaa cccggggcgg
     8701 ttcaggactg gagtgcctta agcgctttat cgaaatcgaa gggttgggac atgtgtcatt
     8761 cctttttggt tgtatattac tggaatgaca cagaatttct aacactccct ttaatttagg
     8821 tttattacta ttattatact tactctcttt tttacatgac ctc
//
LOCUS       DAPHCV010000156         8801 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.157, whole genome shotgun sequence.
ACCESSION   DAPHCV010000156 DAPHCV010000000
VERSION     DAPHCV010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.157"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(424..1113)
                     /locus_tag="RH033_004199"
     CDS             complement(424..1113)
                     /locus_tag="RH033_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003825705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDV2993300.1"
                     /translation="MKTVLDSLKGKLIVSCQALENEPLHSPFIMSRMALAAAQGGAAG
                     IRANSVADISAIKEIVSLPMIGIIKRDYPGSEVFITATLREVDELMTVEPEIIALDAT
                     ARPRPGGQTLEELVTQIRARYPSILLMADIATVEEAVTAERLGFDCVGTTLYGYTAET
                     ARHVLAENDCGFLREVLKAVSVPVVAEGNVDTPECAARCLTLGAHTVVVGGAITRPQQ
                     ITTRFIAAIAS"
     gene            complement(1113..2669)
                     /locus_tag="RH033_004200"
     CDS             complement(1113..2669)
                     /locus_tag="RH033_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDV2993301.1"
                     /translation="MMQMFSGASSGGWFEKAQRFGKSFMLPIAVLPAAGLLLGIGGAL
                     SNPNTLTAYPFLDADWLQAIFTIMTSAGSIVFANLSVLFAVGVAVGLAKSDKGTAGLA
                     SLLAFLVMNATINALLTLNGTLAHKNPASVGQGMTLGIQTLETGVFGGVVIGLVTCTL
                     HSRFNKVSLPQFLGFFSGSRFVPIISSLAAIVVGAVMTVVWPHFQKLIFGLGDLVDAT
                     GYLGTLLYGFILRMLGPLGLHHIFYLPFWTTALGGSEIVNGQLVEGTQRIFFAQLADP
                     NTQQFYAGTARFMSGRFITMMFGLLGACLAMYHTAKPENRKVVAGLLLSAALTSFLTG
                     ITEPVEFSFLFVAPVLYVIHAFFDGLAFMVAHILHITIGQTFSGGLIDFLLFGVLQGE
                     AKTNWMYVPIVGIPWFFLYYFTFRYLINRFGWLTPGRENVTQVESGQPQSERAAAVIA
                     GLGGKENLEEVDCCATRLRVTVKESSKVDEAALKVAGARGVIIRGNGVQVIYGPHVTI
                     IKNEVEEILS"
     gene            2835..3659
                     /locus_tag="RH033_004201"
     CDS             2835..3659
                     /locus_tag="RH033_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDV2993302.1"
                     /translation="MSENENLLLRLRQSVDGYSRTQQKLGEFVLSDPAKVVYLTITEL
                     ARESGTSEASVTRLCRTLGCKGYNEFKMALALDLRQVQFVEHGGDEIDNVVNESVQAI
                     QDTAKLLDRALLEAAALALHQAQSVQIYGVAASAILGEYLHYKLLRLGKPAQLFSDMH
                     RAAMNATTLSKNTFVVAISSSGSTRDLLHVVKLARKQGVRVLALSNTPRSPLASLSDI
                     QLVAAKPEGPLSAGALTAKVGVMLLVELLTTSLIALDDQYSEVSQKTASATLPLLL"
     gene            complement(3816..5159)
                     /locus_tag="RH033_004202"
     CDS             complement(3816..5159)
                     /locus_tag="RH033_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016929550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HDV2993303.1"
                     /translation="MHNSKIRLAILVGCMALSANLWAKAQPVQATLAGHAILPAKSAV
                     STPKDAPGDLQQSGKYTSGKRVTELGSVAGKSAERLTGLSLPINGQPLQGHSGIKHMP
                     DGTYWVLTDNGFGSKANSPDAMLYLNQYKIDFKDGSVVPLKTLFLHDPDKKVPFHIIN
                     ESTELRYLTGSDFDPESFQFSDDALWIGDEFGPYLIKTDLNGKVLAVFDTEVDGKVVK
                     SPDNPTLTLPSAPDGKLNFQVARSKGFEGMAISPDGSKLYPLLEGALWDGEQFENVDG
                     KRYLRVLEFDAKQQAWTGRSWQYVLEDNQNAIGDFNMIDDTHGLVIERDNGEGTADKA
                     CVAGAPTNNCFSQVARFKRVYKIAFSDTNVGKPVEKLGYIDLMKIQDPNKLARKPLND
                     GVLTFPFFTIENVDVVDTNHIIVGNDNNFPFSSSRWPNMADDNEFILLDVKDFLK"
     gene            complement(5518..6515)
                     /locus_tag="RH033_004203"
                     /pseudo
     CDS             complement(5518..6515)
                     /locus_tag="RH033_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612074.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(6654..7970)
                     /gene="ugpB"
                     /locus_tag="RH033_004204"
     CDS             complement(6654..7970)
                     /gene="ugpB"
                     /locus_tag="RH033_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HDV2993304.1"
                     /translation="MKPLHYTASALVLGLTLMGNAQAVTTIPFWHSMEGELGKEVNSL
                     VQRFNAENPDYKIIPTYKGNYEESLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGINFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYSSGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAS
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADIATRQMLNKPSLPFTKGLRLGN
                     MPQIRAIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            8141..8326
                     /locus_tag="RH033_004205"
     CDS             8141..8326
                     /locus_tag="RH033_004205"
                     /inference="COORDINATES: protein motif:HMM:NF014505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDV2993305.1"
                     /translation="MEFDPAVDSEIRKTCPAVIVSNDAANRNLARVVVVPLTSNTERV
                     YPSEAVVTVGVRAAKRL"
     gene            8335..8442
                     /locus_tag="RH033_004206"
     CDS             8335..8442
                     /locus_tag="RH033_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance protein"
                     /protein_id="HDV2993306.1"
                     /translation="MAADKSCLKTHIGSLSKADMLSVEDAIKIHLGLLR"
BASE COUNT         2173 a         2309 c         2155 g         2164 t
ORIGIN      
        1 tgttatagca ataataatga gcacaattgc ttgtgaaact ggtgccattt tacggttgct
       61 taggagtgct gaaactgtgc ctgttagggc acagctggtg tggtggtctc agtacggaac
      121 aatttgaaaa ccagaacatc acttaggctt gtgtccacat tacatggggt aggatcagta
      181 ttcctctatc aggggtaact cgaccaggct ggctgagctc tcgttttact gcgagtatct
      241 atgcccattc agccagtgtc agcgcgagag tgttaaattc cgagcgatta tccatgcgca
      301 gcatgaccgg ataaccatga tttagcccct gccgagctaa tcgggaactg catagcactc
      361 tcaatatgga ataagcgata attatcgctt attcccagca aaatatcatg cctcatcggt
      421 gctttacgag gcgatcgctg caataaagcg tgtcgttatc tgctgcggac gagtgattgc
      481 ccccccaacc accacggtat gagcgcctaa tgtcaggcaa cgtgcggcgc actccggggt
      541 gtcgacattc ccttccgcca cgaccggaac gctgaccgct ttcaggacct cacgtaagaa
      601 tccacagtca ttttccgcca gcacatgcct ggcagtttct gcggtatagc cgtagagcgt
      661 agtgcccaca cagtcaaaac ccagacgttc agctgtcact gcctcttcta ctgttgctat
      721 atctgccatc agaagtatgg acgggtagcg agcgcggatc tgcgtgacca gttcttccag
      781 cgtctgacca ccagggcgcg ggcgcgcagt agcatccagg gcaataattt ccggctcaac
      841 ggtcatcagc tcatccactt cccggagagt ggccgtgata aacacctcac tgccagggta
      901 atcgcgtttg ataatcccta tcatcggaag ggagacaatc tctttgatag cactaatatc
      961 cgccacgctg ttggcacgga tacctgcagc accaccttgt gctgccgcca gcgccatacg
     1021 tgacataata aacggactat gtagaggctc attttccaga gcctgacagg agacgattaa
     1081 tttacctttc agggaatcca gtacagtttt cattacgata aaatctcttc gacttcgttt
     1141 ttaatgatgg tgacatgagg gccataaatc acctgaacgc cgttgccgcg aataatcacg
     1201 ccacgcgctc cggccacttt cagcgcagct tcatctacct tactgctctc tttaaccgtc
     1261 acgcgcaggc gcgtggcgca acagtccacc tcttccagat tctctttgcc tcccagcccg
     1321 gcaatgactg ccgctgcgcg ttcactctga ggctggccgg attcaacctg ggttacgttc
     1381 tcacgccccg gcgtcagcca gccaaagcgg ttaatcaaat agcggaaagt aaagtagtaa
     1441 aggaagaacc acggtattcc cactatgggg acatacatcc agtttgtttt agcttccccc
     1501 tgcaacacgc cgaagagaag gaaatcgata agcccgcccg aaaaggtttg cccaatagtg
     1561 atatgtaaaa tatgggcgac cataaacgcc agaccatcga agaaggcgtg aattacgtat
     1621 aatacgggcg cgacaaacag gaaggagaac tcaacaggct cagtgattcc cgtcaggaaa
     1681 gaggtcaggg ctgccgacaa aagtagtccg gcgacaacct ttctattctc cggctttgca
     1741 gtatggtaca ttgccagaca tgcgccaagc aggccaaaca tcatggtgat aaagcgcccg
     1801 gacataaagc gagcggtacc cgcgtagaac tgctgcgtat tcggatcggc aagttgggca
     1861 aagaaaatcc gctgcgtacc ttcaaccaac tggccgttga cgatttcact gccgcccagc
     1921 gccgtcgtcc agaacggcaa ataaaagata tggtgtaaac ccagtggacc gagcatacgc
     1981 aggatgaagc cgtacaacag cgtccccaga tagcccgtcg catccaccag gtcgcccagt
     2041 ccaaaaatga gtttctggaa atgcggccac accaccgtca taaccgcacc cactactatc
     2101 gcggcaaggg agctgataat gggtacaaaa cgagaaccac taaaaaaacc gagaaactga
     2161 ggtagtgaga ctttattgaa acggctatgt aaagtgcagg tgacgagacc aatcaccacc
     2221 ccaccaaaca caccagtttc cagtgtctga ataccaagcg tcatcccctg gccaactgaa
     2281 gcggggttct tgtgcgccag cgttccgttg agcgttaata aggcgttgat ggtggcattc
     2341 atcaccagaa acgccagcaa tgacgcaagc ccggcagtgc ctttatccga tttcgccagc
     2401 cccacggcga cgccgactgc aaagagtacc gacaggttgg caaacacaat agaacctgcg
     2461 ctggtcatga ttgtgaatat tgcctgcaac cagtcggcat ctaaaaacgg ataagccgtc
     2521 aacgtgtttg gatttgacaa cgcgccacca atccctaata acagccctgc tgcgggcaat
     2581 accgcaatcg gcagcatgaa ggattttccg aaccgctgcg ctttttcaaa ccatcccccc
     2641 gatgaagcgc cgctaaacat ttgcatcatt ttatcatctc ctcgattttt gatgaaaatt
     2701 tttaccataa ttattcttat aaataaaaat caccatcaaa atctaagaag tcgatcatac
     2761 ttttttaaaa tgtctggcat caatcccctc ctttccgtca cactatcaac agataaacgc
     2821 attaaggatt ttacatgtca gaaaatgaaa acctgctgct gagattgcgc cagagcgttg
     2881 atggatatag ccgaacacaa cagaagctgg gagagtttgt acttagcgat ccggcaaaag
     2941 ttgtctacct gacgattacc gaacttgcgc gtgaaagcgg caccagcgag gctagcgtca
     3001 cacgtctgtg ccgaacgctt gggtgtaagg gctataacga attcaaaatg gcgctggcac
     3061 tcgatctccg acaagtacag tttgtagagc acggcggtga tgagattgac aatgtggtca
     3121 atgaatccgt gcaagcaata caggacacag caaaattact cgacagagcc ttgctggaag
     3181 cagccgcgct tgcactgcat caggctcagt cagtgcagat ttatggcgta gcagccagtg
     3241 ccattctggg ggagtattta cattataaac tcctgcgtct ggggaaaccc gcacaattgt
     3301 tcagcgatat gcatcgtgct gccatgaacg ccacaacact tagtaaaaac acgttcgtgg
     3361 tggcgatttc cagttctgga tccacgcgcg atctgctgca tgtcgtaaaa ctagcgcgta
     3421 agcaaggtgt gcgtgtactg gcgctgagta atacaccgcg cagtccgtta gcttcgctga
     3481 gtgatattca actggtcgcg gccaaaccgg aaggaccctt gagtgccggc gcgctgactg
     3541 ccaaagtggg agtgatgctg ttggtagagc tactcaccac atcgctgatt gcgctggatg
     3601 accagtacag cgaggtgagc cagaaaacag ccagcgcgac cctaccgcta ctgctttaaa
     3661 gacataaaaa accgccgaag cgctgggtgc tccccatgaa tcagtgataa taaaccaaca
     3721 tcatattctg gtaggttctg gcgaggtgat taaaaacggt gctcagcacc gttcgttttt
     3781 aaattagcga ggagtatcgt aagatgctct tccccctact tcagaaaatc cttcacatcc
     3841 agtagaataa actcgttgtc atccgccata ttaggccagc ggctggagga aaacgggaag
     3901 ttattatcgt tgcctacgat gatgtggtta gtgtcaacca catccacgtt ctcgatggtg
     3961 aagaacggga aggtcagcac accgtcgttc agcggcttgc gtgccagctt gttcggatcc
     4021 tgaatcttca tcaggtcaat atagcccagt ttttcgacag gttttccgac gttagtgtcc
     4081 gagaaagcaa ttttatacac ccgtttgaac ctggctactt gactgaagca gttgttggtc
     4141 ggcgcgccgg cgacgcaggc tttatccgcc gtgccttcgc cgttatcacg ctcgatcacc
     4201 agtccgtggg tatcatctat catattgaag tcgccgatgg cgttctggtt gtcttccagc
     4261 acgtactgcc agctgcgacc agtccaggcc tgctgtttgg catcgaactc cagcacacgc
     4321 aggtagcgtt ttccgtcaac gttctcgaac tgctcaccgt cccacaatgc cccttccagc
     4381 agcggataca gcttactgcc gtccggcgag atggccatgc cttcaaagcc tttggaacgc
     4441 gccacctgga agttcagctt accgtccgga gcgctaggta gagtcagcgt cgggttgtcc
     4501 ggcgatttaa ccaccttacc gtccacttct gtgtcgaaca ccgccagcac cttgccgttc
     4561 aggtcggtct tgatcaggta aggaccgaac tcatcaccga tccataaggc atcgtcggaa
     4621 aactggaagc tttccggatc aaaatcgctg ccggtcagat agcgtagttc ggtgctctcg
     4681 ttgatgatgt ggaacggcac ctttttatcc ggatcatgca ggaacagcgt cttcagcggt
     4741 actaccgagc cgtctttgaa atctatcttg tattggttga ggtacagcat ggcgtccggg
     4801 gagttggctt tgctgccgaa gccattgtca gtcagcaccc aataggtacc gtcgggcatg
     4861 tgtttgatac ctgagtggcc ctgcaacggc tgaccattga tcggcaggct gagtcctgtc
     4921 aggcgttctg cggatttgcc cgccacgctg cccagctcgg tgacgcgttt gccgctggtg
     4981 tatttgccgc tctgttgcaa atcgcccggc gcatccttcg gtgttgaaac agcggatttt
     5041 gccggcagaa ttgcatgccc ggccagcgtt gcctgcaccg gctgcgcttt ggcccacagg
     5101 ttggcgctca gagccatgca gccaaccagt atagccagcc ggattttgga gttgtgcatg
     5161 tcatactctc ctttcgttgt tatcgcccag aagtaaaact aacgtcattg tagggagcag
     5221 aaatgacaga aatattatga ttacggtagg gatagccgtt gccggcttcc ccgccatgtc
     5281 aacgccggta tttatggttt ctgtttgctt ttctgtaaaa ataaagggga gagacgacgg
     5341 tactataaac aaccatgatg aatctcatct attcggccca gatgaaagac gcgcgtgagg
     5401 ctcttgttcg ttaagcggat acgtatatcc tgtacaataa tcagatatac ctcacagggt
     5461 ctaacacttt atcatcaacg agttgcagat cagatagtca tacggttgct gcgtgcttta
     5521 gctggctgaa tacagcctga aatcatcacc gtaatgacat gatgcccagc acagccttac
     5581 ctttctggcg gcgatggcca caacagcgcg tcagtattct ctgcgttcaa ccagtgaaca
     5641 gacccaacga ctgaatgagt cagtcctttt ctcagcacca atcagtactg aacgagcccc
     5701 ctgaaccaga agcattcgca gatacgaatc accggctttt gttatcctgc ccagcttcga
     5761 cattcagccg ctgctgtatt gtgatagcgt cagcccagcc aggctgccag ttgccgtccc
     5821 tttttaaaat catgtgcatt accgatacag gcaaccagcg cacaggctgt tgtgggacca
     5881 actcctttta gctccatcag ctgtttaata cggatatcat cccttgcagc ctcatgaaga
     5941 cggtggtcat gagacgatat gagttcattt tactctgaca gttcgcacaa caaacggatg
     6001 aagggtcatt ccactgcccc tgggcagtat tccctgctgg attttacgca cgtgccttat
     6061 ccccacaggc agcagcagac caaattcact caacagcccc cggatttcat tacgcaacgc
     6121 cgttcgctcg cggatgattc tggcccggac ccggtattct tcctgtaacg tctgctggga
     6181 ttccgatatg aaaacaacaa atcgcatgcc gggcctgctg acggcctcac agatggcagc
     6241 tgcattatta gcgtcgtttt tgttaccttt gagataagat ttgacaagtt tctacggaat
     6301 aatgcgcacg gtaggcccca tacggaatga gttcccgtgc ccagaatggg aagagccaca
     6361 gacttcgatg ctgatgaggc agggagaaag cccggagaaa aacgtcatca tgtggggagg
     6421 gctcagcgtt tttctccgta ccactattca ttgtgatcaa cgccgtggat ttgaaaaaca
     6481 tttttcggca agatcgaaac caatacgatt aatattcatg actgacttct ccaccaggga
     6541 actgggtaca tctcagtctg gaacgttgat gccgtaagga ggaagatgtc aatcacatcg
     6601 gcctacacgc gatgttagct cagataggcg aaacgtatcc gacattacac tggttaagac
     6661 ttcgtcgatt tctcaaagcg gcgtagtaac tgattcccac gctcaacggc ggtatccagc
     6721 gcctgctgtg gcgtcttctt accggtccac acgctctcaa gctcttcatc cacaatcgct
     6781 ctgatctgcg gcatgttgcc cagacgtagt cctttggtga acggtaacga cggtttgttc
     6841 agcatctgac gtgtcgcgat atccgcccct gggtttttct cgtaaaagcc ctgctcacgg
     6901 gtcaggtcat acgccgcttt ggtgattggc agataaccgg ttttctggtg ccactcggca
     6961 gcattttctg gcttcgcgag gaagtcgagg aacttcgcca cgccggtata cgtttcttta
     7021 tctttgccct gcatcaccca caggctggct ccgccgataa tggcgttttg tggtgcatct
     7081 ttcgcgtcag cgtcgtaagg catcatgcct acgccgtagt tgaatttggc gtactcgcga
     7141 atgctggcaa gagaaccaga agaggcagtc gtcattgcgc aatcaccgtt atagaacttc
     7201 tcggtggatt catccttacg cccgacgtag ctgaaatcgc ctttcttgtt catctcttcg
     7261 agcatggcga tgtgtttcac ctgctccggc ttgttgaact ccagcaccgc atctgtgccg
     7321 tcaaagccgt tgtttttgct ggcaaacggc agaccgttcc aggcgctaaa gttttccagt
     7381 tggatccagc cctgccagcc gctggagtag ccgcacttca taccggaggc tttcagtttc
     7441 gcggcatagt ccgccagatc ctgccaggtt ttcggcggct gttccgggtc taatcctgct
     7501 ttcttgaaag catctttgtt gtaatagaga acaggagtcg agctgttgaa cggctgggag
     7561 agtaagtgtc cggttttact gtcggagtag tagcctgaaa ccgtcggcac aaactgcgac
     7621 tcatcaaagt taatccccgc ctctttaaac acgtcataca ccggtttaat ggctttcgac
     7681 gccatcatgg tggcggtgcc aacttcataa acctgcaaaa tagccggagc gttaccggta
     7741 cgaaatgctg cgatccccgc acttaaactc tcttcgtagt tgcctttata agtcggtata
     7801 attttgtaat ccgggttttc ggcgttaaaa cgttggacca gagaattcac ctctttaccc
     7861 agttcccctt ccatagagtg ccagaacgga atggtcgtca ctgcctgtgc attccccatt
     7921 aacgtcagtc cgagcactag tgctgaagct gtataatgta acggtttcat cgtttatctc
     7981 tcttgcttgt tgtacctgat gagcgaattc acacgtttca tgctcgtggg attaaatatc
     8041 atgcccgaat tgcagaaaaa taactttttt attacatgtg ttagacagta aagtgttaga
     8101 aagatgacag agcagtgacg gcacaggtga gacgtggtgg attgaatttg atcctgctgt
     8161 tgacagtgaa atccgcaaga catgtccggc agtgatcgtg agcaatgacg cagcaaacag
     8221 gaacctcgct cgagtagtcg tcgttcctct gaccagtaac acagagcgag tgtatccaag
     8281 tgaggctgtt gtgaccgtag gtgtcagagc agcaaagcga ttgtagagca gattatggcg
     8341 gcggataagt cctgtctcaa aacacatatt ggctcgttgt ccaaagcaga tatgttgtct
     8401 gttgaggacg caatcaagat ccatcttggt ttactgcggt aacgccatgt gaatctgtcg
     8461 gctaccgcca ttagtgacgg aattttctgt ggggcagcgt accaatggca ttcggttaag
     8521 ttcttgctgg agcatcaaaa gagttccagc gcgttctggg ccgtacatat tttcgtgggt
     8581 aacacctccc tggccttact cgctgagtca tacaactgcg atcgattcag atgccatttt
     8641 gcaatcagcc tctcagaaag agcgcaacag aatgatttta aatgttttcc tgttacccga
     8701 atcagtaaag acaagtacct cactacgaga aacaagcagt ttttgaaatt aaaattttgt
     8761 ttatttctac tactaccgat gaaaacgtta ttatcttaat a
//
LOCUS       DAPHCV010000157         8729 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.159, whole genome shotgun sequence.
ACCESSION   DAPHCV010000157 DAPHCV010000000
VERSION     DAPHCV010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.159"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            356..1066
                     /locus_tag="RH033_004207"
     CDS             356..1066
                     /locus_tag="RH033_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2993307.1"
                     /translation="MTDKTVPFENYSTNYPADRSKADYWSSGTEGYIKIRLDKKIISD
                     VSLNNVLLVSLYASQRQTEHGPVPLFNAWISNLDIQVPQGCTINEGTSFTVNMPDVWA
                     SELSRAGAGAKPAGVTPVATTIPINCTNKDTDAVMTLVFDGNISATRDTNGKQSIIQA
                     QDNPDVGIMIMDSQQNSVDLNALATSVGVPFRLVENQAASAQTADVTFLALPVSTTGK
                     VPAAGRYNALAVLRVEYQ"
     gene            1240..1950
                     /locus_tag="RH033_004208"
     CDS             1240..1950
                     /locus_tag="RH033_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993308.1"
                     /translation="MELVDTLFASLSGTDPFTGVDITIANCKSTYWDEGIIQQLINQV
                     LDEGEKFAGTVGLEGLSRYDVTLNIGLTSSNVWPGFSLDTATIGRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDFTVHFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKEAW
                     KFHTDNSLRGFRDKQAELKLYARHYSVENCTEESCRDCNYCIRPSFSLSRSSIIRLNA
                     ANARFVYQPFTRDQRKRG"
     gene            1953..2728
                     /locus_tag="RH033_004209"
                     /pseudo
     CDS             1953..2728
                     /locus_tag="RH033_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2731..3336
                     /locus_tag="RH033_004210"
     CDS             2731..3336
                     /locus_tag="RH033_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993309.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIALAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMGGIGWQFSRRTPPELKNRLHCWLVFSPAY
                     FWLILSNFILYITPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCQWRSKNRSQGQKWVEWGTCLNLAILAQGPLFNYLQ"
     gene            complement(3390..4895)
                     /gene="glpD"
                     /locus_tag="RH033_004211"
     CDS             complement(3390..4895)
                     /gene="glpD"
                     /locus_tag="RH033_004211"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HDV2993310.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNAEQQSRVSQWLVEYTQQKLSLAS"
     gene            5085..5411
                     /gene="glpE"
                     /locus_tag="RH033_004212"
     CDS             5085..5411
                     /gene="glpE"
                     /locus_tag="RH033_004212"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HDV2993311.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            5456..6286
                     /gene="glpG"
                     /locus_tag="RH033_004213"
     CDS             5456..6286
                     /gene="glpG"
                     /locus_tag="RH033_004213"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HDV2993312.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQNNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            6303..7061
                     /locus_tag="RH033_004214"
     CDS             6303..7061
                     /locus_tag="RH033_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HDV2993313.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(7043..8641)
                     /gene="rtcR"
                     /locus_tag="RH033_004215"
     CDS             complement(7043..8641)
                     /gene="rtcR"
                     /locus_tag="RH033_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HDV2993314.1"
                     /translation="MRKTVAFGFVGTVLDYVGRGSQRWSKWRPSLCLCQQESLVINRQ
                     ELLHDARSRSLFETLKCDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYAFQ
                     PDKEDYLIHITTGTHVAQIFWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRTPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEMNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLSQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTQLLGAEAENIDLFDRLQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFNLTWEAVQDQHSSS"
BASE COUNT         2124 a         2109 c         2221 g         2275 t
ORIGIN      
        1 agggtaccat gtcgtagcct atgctgaaat gggccgggat cgtgagtttt gctatcctgg
       61 tactccagga aacaatacta cgccagcgac ctttttttat gattttggca cagttattat
      121 ttcagatgtt aacaacaatt cagccggtac tgttttacca tctcaaaact ggaaaatagg
      181 tcgatataaa gcatactgta attctcttga tgattatgaa gtttattttt caggtatttc
      241 aggaatagat ccttcaggtg cttcaggtaa tccacaggga gcagaaatat ttattcctgt
      301 tacgcatgaa atatctattt caacacatat aaaactctac aatcaaaatg ggacgatgac
      361 agataaaaca gtaccattcg agaactatag tactaattat cctgctgaca gaagtaaagc
      421 agattactgg tcttcaggaa cagaaggcta tattaaaatt aggttggata aaaaaatcat
      481 ctctgatgtc tctctgaata atgtactttt agtttcattg tatgccagtc agcggcaaac
      541 ggaacatggg ccagtaccat tatttaatgc ctggatcagt aacctggata ttcaggttcc
      601 gcaaggttgc actattaatg agggtactag ttttactgtt aatatgccgg atgtgtgggc
      661 cagtgaattg agccgggctg gtgctggagc gaagcctgct ggtgttactc ctgtagcaac
      721 aactattccg attaattgta cgaataaaga tacagatgcg gtaatgacgt tggtattcga
      781 cggtaacatt tcagctacac gtgataccaa tgggaaacaa agtattattc aggcacaaga
      841 taatcctgat gttggtatta tgattatgga tagtcagcaa aactccgtag atttaaatgc
      901 tctggcaaca tcagtaggcg ttccgttcag attggtggaa aaccaggctg cttctgcgca
      961 aactgctgat gttactttcc tggctttacc tgttagtaca actggaaaag tgcctgcggc
     1021 aggtcgttat aatgcactgg ctgtattgcg agtggaatat cagtaatttt tgagctagta
     1081 cagaatcggt ttagccatca ccgcaaaata aaatgcaata atagggatga acagaatgta
     1141 aaacaaaaaa atatttttaa ttctacaggg acaaaatctg gtggaaaatg taggttatgt
     1201 gccgatggat aaaatgttgc attaagccgg ataataaaaa tggaattagt tgatacgtta
     1261 tttgcaagcc tctccggtac tgatccattc actggcgtgg atattacgat tgccaactgc
     1321 aaatcaacat actgggatga aggcattatt cagcaactca ttaaccaggt cctggatgaa
     1381 ggggaaaagt tcgccggtac tgtcggtctt gagggattat ctcgatatga tgtcacatta
     1441 aatattggct taacctccag caatgtatgg ccgggatttt ccctcgatac cgccacgatc
     1501 ggtcgtcttt gtgcctgtgg tgctgatttt ggatttgacc cctatatcag tgatgtacca
     1561 gatgttcaat gtgacctgaa tacgacaaat gactttaccg ttcattttac ggcaatgctc
     1621 aatccggatg agcgggtgat catagcaaag cgcccgctta aaaaatgtga ctcctggatt
     1681 gaggatgttt atatctttca ggtgtttaaa gaagcatgga aattccatac cgataacagc
     1741 ttacgcggtt ttcgggataa gcaggctgaa ctgaaactct acgcgcgtca ttactcagtg
     1801 gaaaattgca cagaggaaag ctgtcgggat tgtaattatt gcattcggcc cagcttttcc
     1861 ctctctcgta gttccattat tcgcctgaat gcggcgaatg ctcgttttgt ttatcaaccg
     1921 tttacacgcg accagcggaa gagggggtaa ttatgcaacc cagccccgcc atgcaggcat
     1981 taattgagca ggtttatcat atttttcgtc gttatccggt gccgcagcaa tttgtggttt
     2041 gttgtgaata ttgcctgagt caacaggaac aaaaagcatt acggaataca tcgttgcgag
     2101 cgattcctta ttccttaatc aatgctggaa tagtagtcca ggacctgatc cgcaaaacag
     2161 cgatgaggtt cgttattttc ttcctcgttt actggagttt gttgcgcagg ggcaatttga
     2221 caatattcac gaagtatttt ctctacggcg tatcaacctt gccagtaaag aaaactggcg
     2281 tgaggatgaa cgggcaatat tgcagcgatt tgcctgccag tatatggctg attgggtatc
     2341 tggcgatgag gctgtcgaat tacagtatat gctggaaatg ttttcccgtg ccgatatcgc
     2401 tctttcgcca ttgcttgatg ccatcatctc tgtacccggt taccaaccca cagtatcgat
     2461 ggcatgtttg ctgtggcata acagggaaga gaatattcag aatagtcgtt ttgagcagga
     2521 tgatgacgat aaagccatta ccgcccaaat caatgcctgg gccttcaaca atcaatccat
     2581 cctgaaagaa cgcgcgcggc aggcgattga gaacctgctt aatcagcccg aacagggcac
     2641 acattatcag gtctgggaag acgactggat gattgatgag tgtttatgcg cgatgtatga
     2701 cgcatcatcc gaatcaccag gaaaataaaa atgattaaat tccggcttta tattccccct
     2761 gtaattctcg gttttgttat cgtgccatta ttggtatggc cgacggttat tgccttagcc
     2821 gtacttatat tcacgttaac ttttctggct gaaataatat tctcctttcc gctcctggtt
     2881 gtgcgtattt ctcttcagga attacaactt gagttaatgg ttgaatatgc actttttttc
     2941 agtgtaatgg gtggcatcgg ttggcaattc tcccgcagaa cgcctcctga attaaaaaac
     3001 agactgcatt gctggctggt cttttctccg gcctatttct ggttaattct ctcgaatttc
     3061 attctttata ttactccaga gaaatcagcg ttgctggaaa atatccgaaa cttctttctg
     3121 acgtttgtct ggcttcccct gaatttttcc cctttttggc cgcagccgtg gactgatttt
     3181 gtcggcccga ttagtgcaca gcttggtttt gcgttgggat attactgcca gtggcgtagc
     3241 aaaaatagaa gccaggggca gaagtgggtt gagtggggaa cgtgcttaaa tttggcgatt
     3301 ttagctcagg ggccgttatt caattattta caatgaatag tctaaggaat ccaaatagcc
     3361 cggttgcact tcacaaccgg gcatcatcct tacgacgcca gcgacaactt ctgctgcgta
     3421 tactccacca gccactgact cacacgagat tgttgttccg cattcagcca catgccttgt
     3481 tttgtgcgac gccacagggc atcgtcggcg cggcggaccc attcgtgatc caccaggtat
     3541 ttcagctccg cttcgtagaa ctcatgaccg aaatcttcac cgaggtcgct tatcgcccct
     3601 gcattgccga gcagcagttc gctgttgctg ccgtaagtgc gcgcgtagtg acgtgccagc
     3661 gattcggtca ggaacgggta acggcggcgc aggcgggcgg cgtaatcgtc acggtcgcct
     3721 tcaatggcac cacccggcag cacgctctct ttcgtccacg ccggaccaat gccctgataa
     3781 tatggcgtca gtttttccag cgcgtgctcc gccagtttgc ggtaggtggt cagcttaccg
     3841 ccgaataccg acagcagcgg cgctttgccg ttttcatcat ggatatcaag ggtgtaatca
     3901 cgagtgatag cctgcggcga atcggactca tcatcacaca gcggacgcac gccggagtaa
     3961 gtccagacga tatcgtcccg gcttaactgc tttttaaagt gcgtgttata cactttcagc
     4021 aggtaattga tttcactctc ttcaatcttc actgcttttg gatcaccttt gtattccaca
     4081 tcggtagtgc cgatgatgga aaactcgtcc atccacggga tcacgaagac aatacgttta
     4141 tcttcgtttt gcagaatgta ggcctgcttt tgtgtatgaa cgcgcggcac gacaatatgg
     4201 ctgcctttga tcaggcgaat gccataaggc gaaggcagat gcatcccgtc gtcgaagaac
     4261 tgctttaccc acgggccggt ggcgttcacg aggccgcgcg cttgccagct atattttttg
     4321 ccggtatcga tatcttccgc ttccacaatc cacaggccgt tttcgcggcg agcagaagtg
     4381 gcacgagtac gagtaagcac ttcaccgcct ttacgcacca ccatctgggc gttggcgagt
     4441 accagacggg cgtcgtctac ccaacagtcg gaatattcaa atccgcgctt aatttccggt
     4501 tttaatactg aatttgcgcc aaaacgcaaa ccggttgatc ccggcaggct ggtgcgttta
     4561 cccagatgat cgtacataaa cagaccaatc cgaatcatcc acgccgggcg cagatgcgga
     4621 cgatgcggca ggcgaaaacg catcgggaag gcgatatgcg gggccatttt cagtagcact
     4681 tcacgttcag ccagcgcctc gctgaccagg cggaattcat agtgctcaag gtagcgcagg
     4741 ccaccgtgaa tgagttttga actggcggaa gaggtcgcgc aagcgagatc ctgcgcctcc
     4801 agcatcagca cggataaacc gcgtccagcg gcgtctgccg cgataccagc accattgatg
     4861 ccgccaccta tcacaatcag atctttggtt tccatgctgc cctcattcac tttcgttaaa
     4921 gctcacaaat gttcgttatc gaacatatta gcaaagaatc gcgctttggg taacattgaa
     4981 aaaacatttt agagtgatat gtataacatt atggcgttta tcttccgctt cgacgtaaac
     5041 tgtgcggtaa atttgcccac ttgtttgtaa agaaagagag acgcatggat cagttcgaat
     5101 gtattaacgt tgccgacgcg caccagaagt tgcaggaaaa agaggcggtg ctggtcgata
     5161 ttcgcgatcc acagagtttc gcaatggggc atgcggtgca ggctttccat ttaaccaacg
     5221 acacactggg cgcgtttatg cgtgataacg actttgacac tccggtgatg gtgatgtgtt
     5281 accacggcaa tagcagcaaa ggcgcggcgc agtatctgct gcaacagggc tacgatgtgg
     5341 tctatagcat tgacggcggc tttgaagcct ggcagcgtca gtttcctgca gaggtggcgt
     5401 acggcgcgta acgctttata ctgtcccctt ttgtgtggaa taagcgacag caacaatgtt
     5461 gatgattacc tcttttgcta acccccgcgt ggcgcaggcg tttgttgatt acatggcgac
     5521 gcagggtgtt atcctcacga ttcaacaaaa taaccaaagc gatgtctggc tggcggatga
     5581 gtcccaggcc gagcgcgtgc gggcggagct ggcgcgtttt ctcgaaaacc cggcagatcc
     5641 gcgttatctg gcggccagct ggcaggcggg ccataccggc agtggcctgc attatcgccg
     5701 ttatcctttc tttgctgcct tgcgtgaacg cgcaggtccg gtaacctggg tgatgatgat
     5761 cgcctgcgtg gtggtgttta tcgccatgca aattctcggc gatcaggaag tgatgttatg
     5821 gctggcctgg ccattcgatc cgacgctgaa atttgagttc tggcgttact tcacccacgc
     5881 gttaatgcac ttctcgctga tgcatatcct ctttaacctg ctctggtggt ggtatctcgg
     5941 cggtgcggtg gaaaaacgcc tcggtagcgg taagctaatt gtcattactc tcattagcgc
     6001 cctgttaagc ggctatgtgc agcaaaaatt cagcgggccg tggtttggcg ggctttctgg
     6061 cgtggtgtat gcgctgatgg gctacgtctg gctacgtggc gaacgcgatc cgcaaagtgg
     6121 catttacctg caacgtgggt taattatctt tgcgttgatc tggattgtcg ccggatggtt
     6181 tgatttgttt gggatgtcga tggcgaacgg agcacacatc gccgggttag ccgtgggttt
     6241 agcgatggct tttgttgatt cgcttaatgc gcgaaaacga aaataattcc agggatttat
     6301 aaatgaaaca aacacaacgt cacaacggta ttatcgaact ggttaaacag cagggttatg
     6361 tcagtaccga ggaactggtg gagcacttct ccgtcagccc gcagactatt cgtcgcgacc
     6421 tcaacgagct ggcagagcaa aacctgatcc tgcgccatca tggcggtgcg gcgctgcctt
     6481 ccagttcggt taacaccccg tggcacgatc gcaaggccac ccagaccgaa gaaaaagagc
     6541 gcatcgcccg caaagtggcg gagcaaatcc ccaacggctc gacgctgttt atcgatatcg
     6601 gcaccacccc ggaagcggtg gcgcacgcgc tgcttaatca cagcaatttg cgcattgtca
     6661 ccaacaatct caacgtcgct aacactttga tggtaaaaga agattttcgc attattctcg
     6721 ccggtggcga attacgcagc cgcgatggcg ggatcattgg cgaagcgaca ctcgatttta
     6781 tctcccagtt ccgccttgat ttcggcattc tggggatcag cggcattgac agtgacggct
     6841 cgctgctgga gttcgattac cacgaagttc gcaccaaacg cgccattatt gagaactcgc
     6901 gccacgttat gctggttgtc gatcactcga aatttggccg taacgcgatg gtcaatatgg
     6961 gcagtatcag catggtagat gccgtctaca ccgacgcccc gccgccagta agcgtgatgc
     7021 aggtgctgac ggaccaccat attcaactgg agctgtgctg atcctgcacg gcttcccacg
     7081 tcagattaaa acgcgccagg tatttgcgta gccgatccgc gtcgttgacg ctggctttcc
     7141 cctggcgcga aacatcaaaa agctggcgac cggcagcaga aagtgacttt gcctggcggc
     7201 agatggctat tacgtgttcc agttgcagtc gatcaaagag gtcgatgttt tctgcctcag
     7261 cgcccagcag ttgtgtaagc gcggagggac ggctctcctg ccagttatag cgcagacggt
     7321 ttatctcatc ttcaaccacg tccagagtga tgcgtccgct agtggcaaag gtggccatcc
     7381 gcgtgacgct ggcagaaagt tcgcgaaagt taccgcgcca tgttgcctgg ggagaggtcg
     7441 caaaagccag ccaggcgcgc cgcgcttcgg tgttaaaacg cacgctgtcg ccagtgagtg
     7501 aggcgtggcg ctccacttca taatcaaggt tcggttcaat atcttcctgg cgctggctca
     7561 gccccggcaa ggtgaaggtc cagagattga tccgcgcgta cagatcttcg cggaatttgc
     7621 cttcggcaac cagctgacgc aaatcgcgca ccgttccggc gataagctga aagtcgctac
     7681 tcacctggcg atcgctgcca aatgggtaaa aggttttctc ttcaatggct ttcagcaaca
     7741 tcgcctgttc gtctgcgccc agttcgccaa tctcatcaag aaacaacata ccaccgttgg
     7801 cgctgcgtaa taatccttca cgagattccc gcgccccggt aaacgcgcct ttcacatgac
     7861 caaacagcgt cgacatggcg gtatcgccac gcagagtggc gcagttcatt tctacaaatg
     7921 cgccgctaaa ctgatgccgt gcctgtttta attcgaagat gcgtcgtgcc agaaatgact
     7981 tgcccgcacc ggtcgggccg ttaagcagga tcggtgcgcg ggatttaatc gccacttttt
     8041 caatctgctc aatcatgcgg ttgaagtggg gggtacgcgt ggcaatgccg gacttaagaa
     8101 aatcgagcgt ttgctgccgt tcctcggcaa agcggctggc gatggcgtta taacggctta
     8161 aatcgagatc gataatcgtc acttcacctg cgccgcgcgg ctgttctttt ttgcgcggag
     8221 gggaagattg tatcagccgg gcgggcaggt aacgcgcttc cgccagcaga aaccagaaaa
     8281 tctgtgcgac gtgggtgcca gtggtgatgt gaattaaata gtcttcttta tcaggttgaa
     8341 acgcgtagcc acgggcgaaa tcatgcaggc aggcgtaaac ctcttcgaaa tcccacgggt
     8401 tatgcagttc aatctcaacg ctcaccactt cagtttctgg cgaaacgctg gcgatatcgc
     8461 atttaagcgt ttcaaacagc gagcgcgagc gggcgtcgtg caacaattcc tgtcgattga
     8521 tgactaacga ttcttgctgg catagacaga gtgacggacg ccatttcgac cagcgctgac
     8581 tgccgcgccc gacataatcc agcaccgttc cgacaaagcc aaaagccact gttttacgca
     8641 tcttagatat ccttataaaa gacgagattt cattatcggt aaatcggctt tgcgggaaat
     8701 ctccggttac ggacgtaaag atataatct
//
LOCUS       DAPHCV010000158         8637 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.160, whole genome shotgun sequence.
ACCESSION   DAPHCV010000158 DAPHCV010000000
VERSION     DAPHCV010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.160"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..330)
                     /locus_tag="RH033_004216"
     CDS             complement(<1..330)
                     /locus_tag="RH033_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HDV2993315.1"
                     /translation="MKLNARQIDTAKPKEKAYKLADGGGLYLLVKPGGGEYWRLKYRV
                     AGKEKLLALGVYPEVTLADAPAKLEEAKRGISGGIDLMEVKREEKIARETQLNNTFKD
                     IALEWHSN"
     gene            923..2062
                     /locus_tag="RH033_004217"
     CDS             923..2062
                     /locus_tag="RH033_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020219271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993316.1"
                     /translation="MKMTKADFGVTIDDTLITDFRDVVNEGEFAYSYFINRNGKNQFS
                     PICSCMDWISVSVRNLMNFPELSEDIDVKAMQVYSLISSIDIAVEALQQLHRIIESDD
                     KLARWPFKKSTNVFKNKTPYLSHEDDDAYFKSIRAIFGAHPTNLKNSDGERLFASWPH
                     FHAFNENDFTISLYNNTPGKDDVIFGIKFDELITYIEDRYKYLTSLKGSVIAIRIKHY
                     STLSKQIIPSTDNIHAELKLLLSEVAVRGDNDYYRMEVEELIKLFEGNVKEAHLKDEA
                     DVFLSKLHPIAVEIRYNLQEMNIEDLATKHGVLISTLPKNALHYVLQKMFTWLHSDRY
                     DPMGGFYLDKLNEYSGGRYNFCSEDDDSTTLLKLRMMLHWYHQQK"
     gene            complement(2111..2808)
                     /locus_tag="RH033_004218"
                     /pseudo
     CDS             complement(2111..2808)
                     /locus_tag="RH033_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(3111..3473)
                     /locus_tag="RH033_004219"
     CDS             complement(3111..3473)
                     /locus_tag="RH033_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RnlB family
                     antitoxin"
                     /protein_id="HDV2993317.1"
                     /translation="MFEITGVNVSGALIAVVMATGYENPLTSVHEIEKRLSVILGNSQ
                     ATGEVLFDLLCSNGPEWNRFVSLEIRCGSILLDTAKIVDEQEIPAQILRHQRDVLRNH
                     PEYVEDSVLTPDDVLCIL"
     gene            complement(3466..4536)
                     /locus_tag="RH033_004220"
     CDS             complement(3466..4536)
                     /locus_tag="RH033_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RnlA family
                     toxin"
                     /protein_id="HDV2993318.1"
                     /translation="MTVRNYTNLNLDRTTIEATSRAFIENKNYSVHSIGPMPGARAGL
                     RVVFAKPGEALATVNIFYNNGGTSTVQYQTGANHNLGKELADDLYETINPAEFEQVNM
                     VLQGFVEANVLSVLQLSAEQPHIQFYEYLRNTHTTVWKIHSPEFQDELTVSLHHRNGT
                     LQIQGRPLSCYRVFIFNLSELLDLQGLEKVLIRQDDGKAYIVQKEVARSHLECEMGDA
                     YPLLHKNVEKLLVSGLCVKLAAPDLPDYCMLLYPELRSVEGVLKSTMYRYGMPITQDG
                     FGRYFDKIGSEFILKQQFGCNLSSAKVKTINDAYTFFNRERHGLFHMEIVVDTSRMVS
                     DMARLMTKARQAWGIIKDLYIV"
     gene            complement(4596..4751)
                     /locus_tag="RH033_004221"
     CDS             complement(4596..4751)
                     /locus_tag="RH033_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993319.1"
                     /translation="MRHLVVLVEELRERGINFCNLTDSIDTQYLVNTNTWLQEGYYRY
                     PISGYSA"
     gene            complement(4937..5521)
                     /locus_tag="RH033_004222"
     CDS             complement(4937..5521)
                     /locus_tag="RH033_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDV2993320.1"
                     /translation="MAFRMSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNNLMQVASEHIAPLQDAADLEIATEGE
                     TSLLEAWKKYRVLLNRVDTSIAPDIEWPASPVME"
     gene            complement(5521..>8637)
                     /locus_tag="RH033_004223"
     CDS             complement(5521..>8637)
                     /locus_tag="RH033_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HDV2993321.1"
                     /translation="EDDARPEALRRFELMVEEVARNASVVAQNTAAAKKSASDASTSA
                     REAATHATDAAGSARAASTSAGQAATSAQSASSSAGTASTKATEASKSAAAAESSKSA
                     AATSAGAAKTSETNAAASQKSAATSASAATTKASEAATSARDAAASKEAAKSSETNAS
                     SSASSAASSATAAGNSAKAAKTSETNARSSETAAGQSASAAAGSKTAAASSASAASTS
                     AGQASASATAAGKSAESAASSASTATTKAGEATEQASAAARSASAAKTSETNAKASET
                     RAESSKTAAASSASSAASSASSASASKDEATRQASAAKGSATTASTKATEAAGSATAA
                     AQSKSTAESAATRAETAAKRAEDIASAVALEDASTTKKGVVQLSSATNSTSETLAATP
                     KAVKAAYDNAEKRLQKDQNGADIPDKDRFLSNINVYSKGEVDQKKGMRYVVVNAPAGV
                     QEGKYYPLVIKRNDSHRASRVVISTPSRTANHRMNNCEFNGFVCAGGWTDRGSYACGM
                     FWAYSSSERAIHSILMSNKGDTVDSVFYIEGGAFPVEVFLEEGLSVTAPASDYVVAET
                     TYKFGATDPYSESVAVNLILDFKQGNGFYSSYPVLSKSDISGNKIYANDEVIVRSQNA
                     LRMIAGDYGVIWRNDGANTYLLMTDKGDQYGGWNGLRPFAVNNATGEVTINTPLNSPK
                     GIKGNSDTATKLQTAIKISGVSFDGSTDITLTAAHVAAFARRATDTYADADGGVPWNA
                     ESGAYNVTRSGDSYILVNFYTGVGSCRTLQMKAHYRNGGLFYRSSRDGYGFEEDWAEV
                     YTSKNLPPESYPVGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRG
                     WTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTH
                     SISGTANSAGAHQHKSSGAFGGTNTSIFPNGYTAISNPSAGIMSTTSGSGQTRNAGKT
                     SSDGAHTHSLSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIA
                     FNYIVRLA"
BASE COUNT         2153 a         2150 c         1927 g         2407 t
ORIGIN      
        1 gttactgtgc cactcaaggg caatatcttt gaaggtgttg tttaactgcg tttcccgggc
       61 aatcttttcc tctcgcttca cttccatcag atcgattccc ccagagatac ctcttttagc
      121 ttcttcaagt tttgcaggag catcagctaa ggtgacctca ggatacacac ctagtgctaa
      181 cagcttctct ttaccagcta cacgatactt gagacgccaa tattctcctc caccaggttt
      241 taccaggagg tacagaccac caccatcagc caacttgtaa gccttctctt ttggcttggc
      301 agtgtctatt tgacgggcat tgagtttcac ttgggggtat ctccactaaa ctgaacagca
      361 aatcccctca attgcttgta gagtttgggg tacttaaata gacgtcaaaa gactaaaagg
      421 ggcattaata tactgattat aagaggtttt taaatacttg agtagacttg gggagacgtt
      481 agaatggtgc cgataatagg agtcgaacct acgaccttcg cattacgaat tataagaatc
      541 cgcttctaat tcaaagcatt accccatcaa cactgcgctc acaagtccca ccacatcaaa
      601 acatgtaaag ccttgcaagc cattgctagg ccttatgtgt ctcagttttg tcccactatg
      661 acccgcataa ccaataagaa gattgcagtg atgtcaaacg acacagcagc cttcttttcc
      721 ttcatacttt ccccacccag catgcatacc ttctaccata actgtagtga atgtctgtta
      781 tgagcgagga gcggaagttg gaatgcatac ttaaatcgag caattttgag ttatgggggg
      841 gctggtcaac ctactgaatt gtttttgcct atgataacgg tcacaatcaa cctctaagtg
      901 tgctgatttg aaagtgattt ccatgaagat gaccaaggca gattttggtg taactattga
      961 tgatacttta ataactgatt ttcgagatgt ggttaatgaa ggggagtttg cttacagcta
     1021 cttcataaac agaaatggta aaaaccagtt cagccctatc tgttcgtgca tggattggat
     1081 aagcgtttct gttcgcaatc tgatgaactt ccctgagctg tcggaagata ttgatgtcaa
     1141 ggccatgcag gtttactccc ttatatcatc tattgatatt gctgtggagg cgttgcagca
     1201 gcttcaccgc atcattgaga gtgatgataa gttagcgagg tggcctttca agaaatcaac
     1261 gaatgtcttt aagaataaaa cgccctatct ttcacatgag gatgatgacg catactttaa
     1321 gagtattcga gctatctttg gtgctcaccc tacaaatctt aaaaatagtg atggtgagcg
     1381 actgtttgca agctggcctc attttcatgc tttcaatgaa aatgatttca ctataagcct
     1441 ttacaacaac acgccaggga aagatgatgt tattttcggc attaaattcg atgagctaat
     1501 tacatatatt gaagataggt ataaatatct aacaagccta aagggttccg taatagcaat
     1561 ccgtataaaa cattacagca ccctatccaa acagattata ccgtctactg ataacattca
     1621 tgctgagtta aagttgcttc tttcagaagt tgcggtgcga ggtgataacg attattaccg
     1681 aatggaagtg gaagagttaa taaagttgtt cgaggggaat gttaaagagg cacatctcaa
     1741 ggatgaagct gacgtatttt taagtaaact acatccaatt gcagtagaga ttagatataa
     1801 tcttcaggaa atgaatattg aggatttagc aactaagcat ggcgtgttga tttcaacact
     1861 tccgaaaaat gccctgcatt acgttcttca gaaaatgttt acttggctcc actctgatag
     1921 atatgaccca atgggtggct tttacttgga caagttaaat gaatattcag gcgggcgata
     1981 caatttttgt tctgaggatg atgacagtac aactctattg aagctccgga tgatgttgca
     2041 ttggtatcat caacaaaaat aggcttagcc acgaaaacaa gcaatacagt tggaaggtaa
     2101 tgactccaac ttattgatag tgttttatgt tcagataatg cccgatgact ttgtcatgca
     2161 gctccaccga ttttgagaac gacagcgact tccgtcccag ccgtgccagg tactgcctca
     2221 gattcaggtt atgccgctca attcgccgcg tatatcgctt gcttattacg tgcagctttc
     2281 ccttcaggcg ggattcatac agcggccagc catccgtcat ccatatcacc acgtcaaagg
     2341 gtgacagcag gctcataaga cgccccagcg tcgccatagt gcgttcaccg aatacgtgcg
     2401 caacaaccgt cttccggagc ctgtcatatg cgtaaaacag ccagcgctgg cgcgatttag
     2461 ccccgacata gccccactgt tcgtccattt ccgcgcagac gatgacgtca ctgcccggct
     2521 gtatgcgcga ggttaccgac tgcggcctga gttttttaaa tggcggaaaa tcgtgttgag
     2581 gccaacgccc ataatgcggg cggttgcccg gcatccaacg ccattcatgg ccatatcaat
     2641 gattttctgg tgcgtaccgg gttgagaagc ggtgtaagtg aactgcagtt gccatgtttt
     2701 acggcagtga gagcagagat agcgctgatg tccggcggtg cttttgccgt tacgcaccac
     2761 cccgtcagta gctgaacagg agggacagct gatagaaaca gaagccactg gagcacctca
     2821 aaaacaccat catacactaa atcagtaagt tggcagcatc accacgtgaa tctccatgat
     2881 ttcttcgcca ctgcgcggct tcaatctaag tacaattcat ttgcagcatt gtctgcagca
     2941 gcaaccgcgc tattccagat tattggtttg taatgcttac cagccccaaa ttaatcaata
     3001 cccagtggta ttagcaatgt ccatttcaag tacatacgtt cttaagacat ttgggcactg
     3061 tcatgtctgt cggggaggag gttaaaatcc tctcgtgcaa aatttcttag ctagagaata
     3121 caaagaacat catctggtgt taacacagag tcttctacgt actcagggtg gttacgtaat
     3181 acatctctct gatgtctcaa gatctgcgct ggaatttctt gctcatccac aattttggca
     3241 gtatccagca aaatactgcc acaccttatt tccagcgaaa caaagcgatt ccactctgga
     3301 ccattagaac acaaaaggtc aaacaaaacc tcacctgtag cctgtgaatt gcccagtata
     3361 acagaaagcc tcttctcgat ttcgtgaact gaagtcaagg ggttctcgta gccagtcgcc
     3421 ataacgacgg caatgagagc ccctgagacg ttaacacctg ttatttcaaa caatatacaa
     3481 atccttaata attccccatg cctgtctggc cttagtcatc agtcgtgcca tgtcagaaac
     3541 catacggctt gtgtcgacaa ctatctccat atgaaacagg ccatgacgtt ctctgttaaa
     3601 gaaagtatag gcatcattaa tggtcttaac cttagctgac gataaattgc atccaaattg
     3661 ttgtttcaga ataaattcac tcccaatttt atcaaaataa cgcccgaaac catcctgcgt
     3721 gattggcatg ccatatcggt acattgttga tttaagtact ccctcaactg aacgtaattc
     3781 aggatacaac aacatacagt aatcgggtaa atcgggggcg gcgagtttaa cacacagccc
     3841 agaaacgagc aatttttcaa cattcttatg caatagtggg tacgcatccc ccatttcaca
     3901 ctctagatgt gagcgagcaa cttctttctg aactatatac gctttgccat cgtcctgacg
     3961 aatgagaact ttttccagtc cttgtaaatc gagtagctct gacaagttga atataaacac
     4021 tctataacat gacaatggac gcccttgtat ttgtagtgtt ccatttctgt gatgcagact
     4081 aactgtcaac tcatcctgaa attctggcga atgaattttc cacaccgttg tatgagtgtt
     4141 ccgtaagtat tcgtaaaatt gtatatgtgg ctgctctgct gagagctgaa gtacagataa
     4201 aacgttcgct tcaacgaacc cttgtaacac catatttacc tgttcaaatt ctgcaggatt
     4261 gatggtttca tagagatcat cagccaactc ttttccaaga ttatgattag ctcctgtttg
     4321 atactggact gtagacgtcc ctccgttatt atagaaaatg ttaacagttg ccaaagcttc
     4381 tcctggttta gcaaacacaa cccgaagtcc tgctcttgca ccaggcattg gtccaattga
     4441 atggacggaa tagtttttat tttcaataaa tgccctagac gtagcctcaa tagttgtccg
     4501 atctaaattt aaattagtgt agttcctgac tgtcatttgc ttaaccctat aaaaaaattt
     4561 agcaaactct acaatatagc ccctctttag tctaatcagg cgctgtatcc tgaaatcggg
     4621 taccgataat atccctcctg cagccatgtg ttggtgttaa caagatactg tgtatcaatt
     4681 gaatccgtca gattgcaaaa gttgatgcct ctttcgcgca gttcctccac cagcacgaca
     4741 agatgccgca tactgcgcct cagtcgatcc agtttccaga ccaccagagt gtcacctgcc
     4801 gataatgtcc tgagaaattt tttagcccgg gtctttcggg cgttgtaacg ctgattttat
     4861 cttcaaaatc agcgcgcatt cttcttaggc cagcaagttc ttatatgtga cgtgactttg
     4921 gctacaacag atacgattat tccataacag ggcttgcagg ccactcaata tccggtgcaa
     4981 ttgatgtatc aacacggttc agcaacaccc gatacttttt ccaggcttcc agcaacgagg
     5041 tttctccctc cgttgcgatt tccagatctg cagcatcctg aagcggcgca atatgctcac
     5101 tggctacctg catcaggttg ttttttgttt cttccgcctc ccggatccgg aacagttttt
     5161 ctgcttccgt atccttcacc caggctgtgc cgttccactt ctgaaactcc ccttccgggg
     5221 ataaccaggt gacattttcc ggtaatgagc cgagttcaga aataaataac gcgtcgccgg
     5281 aagccacgtc atagaccgtt ttaccccgat gatcttcaac gagatgccac gatgcctcat
     5341 cactgttgaa aacagccacg aagcctgccg gaatatctgg tggtgcaata tcggtactgt
     5401 ttgctggcag acctgtatga ggcggaatat atgcgtcacc ttcaccaata aattcattag
     5461 ttccggccag cagattataa atttttatgg tccgtgcttg ttcactcatt ctgaatgcca
     5521 ttatgcaagc ctcacaatat agttaaatgc gatgtttttg acggtgtttt ccgcgttacc
     5581 cgcagcgtta acggtgatgg tgtgtccatg tgagccaatc gcaacggagt gcgtatgagc
     5641 accaataccg acagtatgtg catgtgcgcc tgcgcttgca gcagtgccgg acagcgagtg
     5701 ggtatgagca ccatctgatg atgtcttccc tgcattacga gtctggccac taccgcttgt
     5761 tgtgctcata atccccgcgc ttggatttga aatcgcggta taaccattag ggaaaatgct
     5821 cgtgttcgtg ccaccaaatg caccggaact cttgtgttgg tgcgcgccgg cactatttgc
     5881 ggtcccgcta atactatggg tatgcgcccc ggtgttattc gtggatttag tgccgtaatc
     5941 aaacgacgat gtggttttcg tccccaaatc agtactggat gcgctggcgc tgtgggtatg
     6001 cgattttatg ccgtcctgtt cctgagacaa tacggcacga ccactggcgg gcttgccctt
     6061 aatcgtccag ccacgcatat cagggatcac gcctgacgga taagcggctg caagtttcgg
     6121 gtaggcagat ttgtcaaaag tctgcccctg catcagggca taaccagacg gaacggtatc
     6181 tgatggccac gggattggtg cgccgactgg gtagctttct ggtggaagat ttttcgaggt
     6241 ataaacttct gcccagtctt cctcaaaacc ataaccgtct cttgaagaac ggtagaacag
     6301 accaccattt ctgtaatgcg ccttcatctg caaggtccgg caacttccga ctccggtata
     6361 gaagttaacc agaatatagc tgtcgccaga gcgggtgaca ttataagcgc ctgattcggc
     6421 attccaggga acgcccccat ccgcatcggc atacgtatcc gttgctcttc tggcaaaagc
     6481 agcaacatgc gcagcggtta aagtgatatc agtagaacca tcaaacgaaa caccagatat
     6541 ttttattgct gtctgaagtt tcgtagctgt atcagaattt cccttaatcc ccttaggcga
     6601 attaagtggt gtattgatcg tcacttcacc tgttgcgtta tttacagcaa atggtcgaag
     6661 accgttccag ccaccgtact ggtcgccttt atcagtcatg agcaagtaag tatttgctcc
     6721 atcatttcgc catatgacac cgtaatcgcc tgcaatcatc cttaacgcgt tctgactacg
     6781 gacaataact tcatcattag cgtaaatctt attcccgcta atatctgact tactcaaaac
     6841 aggatacgaa ctataaaatc cattaccttg tttaaaatct aaaattaaat ttacagcaac
     6901 tgattcagaa taaggatcag ttgcaccaaa tttataagtt gtttcagcaa caacataatc
     6961 agaagcaggt gcagtaacgg agagtccttc ttctaaaaaa acctctacgg gaaatgcccc
     7021 accttctata tagaatacgc tgtctacggt atcgccttta ttactcatca gaatggaatg
     7081 aatagcgcgt tccgatgatg aataagccca gaacatacca caggcatagc taccccggtc
     7141 cgtccagccg cctgcgcaaa caaaaccatt aaactcacag ttattcattc tgtgattagc
     7201 tgtgcgactt ggcgttgaaa tgacaacgcg ggatgctcga tggctgtcat tcctttttat
     7261 cactaacgga taatatttac cttcctggac acccgccggg gcatttacca caacgtatcg
     7321 catccccttt ttctgatcca cttcaccttt gctgtaaaca ttaatgttac tcaggaagcg
     7381 gtccttgtcg ggaatatcag caccgttctg gtctttctgc agacgtttct ctgcattgtc
     7441 ataggctgct tttactgcct ttggcgttgc cgccagcgtt tcagacgtgc tgttggtcgc
     7501 actgctgagc tgtactaccc cctttttcgt cgtgctcgca tcctcaagcg ccacggcgga
     7561 tgcaatatcc tctgcacgtt ttgccgctgt ctcagcgcgc gttgccgcgg attccgccgt
     7621 acttttgctc tgtgctgccg ccgtcgcact gccagctgcc tctgtcgcct tcgtggatgc
     7681 tgtcgtggcg ctgcccttcg ctgctgacgc ctgtctggtc gcctcatctt ttgaagcaga
     7741 cgccgatgat gccgatgacg ccgccgaact ggctgacgat gcggcagccg tttttgagga
     7801 ttctgcacgg gtttccgacg ctttcgcgtt cgtttcggat gtcttcgctg cggaagctga
     7861 cctcgctgct gcgctggcct gttcagtggc ttcgccagcc ttcgttgtgg ctgttgaagc
     7921 agacgatgcg gcactttctg ccgactttcc ggcagcggtg gcactggctg aggcctgccc
     7981 ggcacttgtt gatgcggcac tggcagatga tgcagccgct gtttttgagc ctgccgcagc
     8041 tgaggcactc tgtcccgctg ccgtttcaga agacctggcg ttcgtctcgg acgtttttgc
     8101 cgccttcgcg gaatttcctg ccgccgttgc cgaggaagcg gcactactgg cgcttgatga
     8161 tgcgttcgtt tctgatgatt tcgctgcctc ttttgaggcc gccgcatccc gggctgaggt
     8221 ggcagcttct gacgcctttg tggtcgctgc ggatgcagaa gtggctgcag atttttgtga
     8281 cgctgccgca ttcgtttctg acgttttcgc cgcaccggca ctggtagctg ccgcgctttt
     8341 tgaggactct gcagcggcag cactttttga ggcttcagtg gcctttgttg atgccgttcc
     8401 tgcgctggaa gacgctgact gagccgacgt cgcggcctgc ccggctgatg tgctggctgc
     8461 gcgtgctgag cctgcagcat cagtcgcatg ggttgccgcc tcacgggctg atgtgctggc
     8521 atcactggct gacttcttcg cggctgccgt gttctgtgcc accacggacg cgttacgcgc
     8581 cacctcttcc accatcagct caaaacggcg cagtgcctcc ggacgggcat catcctc
//
LOCUS       DAPHCV010000159         8603 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.161, whole genome shotgun sequence.
ACCESSION   DAPHCV010000159 DAPHCV010000000
VERSION     DAPHCV010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.161"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            24..1244
                     /locus_tag="RH033_004224"
     CDS             24..1244
                     /locus_tag="RH033_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid deaminase"
                     /protein_id="HDV2993322.1"
                     /translation="MKYHSEPLVPHKSALMQMPANLLAEDVCLPAAIIKKQALENNIT
                     WMQRYADARGVSLAPHGKTTMTPWIFQAQQAAGAWAIGVGSAWQAGAAMASGIQRVLM
                     VNQLVGKANMQLIAQLQRHYPTVDFICCIDSIENARALSAFFASQQQTLNVMIELCVP
                     GGRCGCRSRDAALTLAKQAAQLPGLRLRGLELYEGVLHGDDPQPQVEALLRDAAQLAC
                     DMASLVDGEFILTGAGTVWYDVVCNIWLAAEKPDNCRIVIRPGCYITHDTGIYDAAQQ
                     QLLARDPVACDLAGDLTSALELVAMVQSVPEADRAVVNFGKRDCAFDAGLPQPVAHYR
                     NGKSLAFDPQAIRSTGIMDQHCMLQLSANSDVQVGDILVFGTSHPCLTFDKWKTLLLT
                     DDDYNVLAELDTLF"
     gene            1512..3662
                     /gene="btsT"
                     /locus_tag="RH033_004225"
     CDS             1512..3662
                     /gene="btsT"
                     /locus_tag="RH033_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HDV2993323.1"
                     /translation="MDTKKIFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            3712..3915
                     /locus_tag="RH033_004226"
     CDS             3712..3915
                     /locus_tag="RH033_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HDV2993324.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            3926..4882
                     /gene="yjiA"
                     /locus_tag="RH033_004227"
     CDS             3926..4882
                     /gene="yjiA"
                     /locus_tag="RH033_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HDV2993325.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLRK"
     gene            complement(4916..5218)
                     /locus_tag="RH033_004228"
     CDS             complement(4916..5218)
                     /locus_tag="RH033_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HDV2993326.1"
                     /translation="MSMVKHKRGTSSTLDVQHEAELKVLANKSDEEIDYSDIPPSSDE
                     QWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKGYQTRLNAILREAMLRDQNKK"
     gene            complement(5215..5435)
                     /locus_tag="RH033_004229"
                     /pseudo
     CDS             complement(5215..5435)
                     /locus_tag="RH033_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
     gene            5531..6697
                     /locus_tag="RH033_004230"
     CDS             5531..6697
                     /locus_tag="RH033_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HDV2993327.1"
                     /translation="MKNYKVITPLFPTYAQVKAMMKAVSGYSLKAVRNMITAIHEQTG
                     TPQKPVDWSEPDLWISERLTGEDADIARRIWDTDNHILNPRHSYGCYLFLNYPQFELM
                     ESTPDDTWQPTSRGQKFLQDDEKTLRSLDDQEGILQLLELLAGREMSRRADLLPEWQA
                     FLHQHSKFASASSVKSTLYSRLYNLIDRDMVNREGMSYRITDTGRAWLGKALPARKSD
                     PRKELLEAVKRYNEQQKELLREQISTMNPYKFEQLVGQLLEAMGYEQVEVTKASGDKG
                     VDVIGQVQVGITTITEVVQVKRMQNTITRPYIDQLRGALPYHKAIRGTLITTGKFAAK
                     CAEAALFPGAAPITLIDGDRLLELLIENNVGIRRSNAVELLDVDLQLFDELEMD"
     gene            6761..8380
                     /locus_tag="RH033_004231"
     CDS             6761..8380
                     /locus_tag="RH033_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDV2993328.1"
                     /translation="MAKAPTKKAKAKKGFEETLWDTANQLRGSVESSEYKHVVLSLVF
                     LKFISDKFEARRKKMIADGQADFLEMEVFYQQDNIFYLPEEARWSFIKQNAKQDDIAV
                     RIDTALSTIEKRNPTLKGALPDNYFSRQNLETKKLASLIDTIDNIETLAHETDVETLS
                     KEDLVGRVYEYFLGKFAATEGKGGGEFYTPKCVVTLLTEMLEPFQGKIYDPCCGSAGM
                     FVQSVKFVESHQGKSRDIALYGQELTATTYKLAKMNLAIRGLSANLGERPANTFFSDQ
                     HPDLKADYILANPPFNLKDWRNEAELTKDPRFAGYRMPPTGNANYGWILHMLSKLSAN
                     GTAGFVLANGSMSSNTSGEGEIRAQMIENDLIDCMIALPGQLFYTTQIPVCLWFMTKS
                     KAADPAKGYRDRQGETLFIDARNLGTMISRTTKELTTEDIATIADTYHAWRSTPEELA
                     ARIARGDSKLEKYEDQAGFCKVATLQDIKDNDYVLTPGRYVGAAEQEEDGVAFETKMR
                     ELSKTLFEQMKQAEELDRAIRQNLEALGYGL"
     gene            8370..>8603
                     /locus_tag="RH033_004232"
     CDS             8370..>8603
                     /locus_tag="RH033_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDV2993329.1"
                     /translation="MGFDFQLLENAGIQLIDGDRGKNYPKHNDFMENGYCLFLSAKNV
                     TKSGFQFQETLFINETKDRELRAGKLKYGDIVLT"
BASE COUNT         2081 a         2232 c         2251 g         2039 t
ORIGIN      
        1 gaaaaactta aaaggtaaat gttatgaaat accactcaga acctctggtt ccccataaat
       61 ctgccctgat gcaaatgccc gcaaatctcc ttgccgaaga tgtctgttta cctgccgcga
      121 tcattaaaaa gcaggccctg gagaacaaca ttacgtggat gcaacgctac gctgacgcgc
      181 gcggcgtttc actggcaccg cacggtaaaa ccaccatgac gccgtggatt tttcaggcgc
      241 agcaggcggc tggtgcctgg gcaataggtg tcggtagcgc atggcaggct ggtgcggcaa
      301 tggcgagcgg tattcagcga gtgctgatgg ttaaccagct ggtcggcaag gcgaatatgc
      361 agttgattgc ccagttgcaa cgacattacc cgacggtcga ttttatctgc tgtatcgaca
      421 gcattgagaa cgcccgcgct ttgtctgcct tttttgccag tcagcaacag acactgaacg
      481 tgatgattga gttgtgtgtc cccggcgggc gctgtggttg ccgctcaagg gatgccgcac
      541 tgacactggc taaacaggca gcccaattac cggggttacg cctgcggggg cttgagttat
      601 atgaaggcgt tttacacggt gacgatccac aaccgcaggt agaagctctg ctgcgggatg
      661 ccgcacaact ggcctgtgat atggcaagtc tggttgatgg cgagtttatt ctcaccggag
      721 caggcacggt ctggtatgac gtggtgtgca atatctggct ggcggcagaa aagcctgata
      781 actgccgcat tgttattcgc cctggctgct acatcactca cgacaccggg atctacgacg
      841 cggcacaaca acagttgctt gcccgtgatc ctgtggcctg cgatctggca ggcgatctca
      901 catctgcgct ggaactggtc gccatggtgc aatcggtgcc ggaagctgat cgtgcggtgg
      961 taaattttgg taagcgtgat tgcgccttcg acgccggact gccgcaacca gtcgcccact
     1021 accgcaacgg caaatcactg gcatttgatc cacaggcgat tcgcagtaca ggtattatgg
     1081 atcagcactg catgttgcaa ctgtcagcta atagcgacgt gcaagtgggg gatattctgg
     1141 tgtttggcac atcgcatccg tgcctgacct tcgacaaatg gaaaacgttg ttattgactg
     1201 atgacgacta caacgtactg gcagaattag acactctctt ctaagaacgc cccttcggtt
     1261 aattatcgaa ggggcatttt tattgtgatc tcaatcacat atccacccga cgaataattc
     1321 ttgcccaacc gctcaccgcc attcctgacc gcttacgtcc ctaaaataac cactcagtta
     1381 tttaccttac tttacgcgcg cgtaactctg gcaacatcac tgcaggatag cggtcaattt
     1441 acctcctcaa acgcaacgca aacctagaac ggcttcggcc aactattaat caatacatgc
     1501 caggttttac tatggatact aaaaagatat tcaagcacat accctgggtg attctcggaa
     1561 tcatcggtgc attctgcctc gcggtagttg cattacgtcg gggggagcac gtcagcgccc
     1621 tgtggatcgt ggtcgcctct gtgtcggtgt atctggtggc ttatcgctac tacagtctgt
     1681 acatcgccca gaaggtgatg aaactcgacc ccacgcgcgc gacgcctgcg gttattaaca
     1741 acgacggtct gaactacgtt ccgaccaacc gttacgtgtt gtttggtcac cacttcgccg
     1801 ctatcgccgg tgcaggtccg ctggtgggtc cggttctcgc cgcgcagatg ggctacctgc
     1861 ctggcacgct gtggctgctg gcgggggtcg tgctggccgg tgcggttcag gactttatgg
     1921 tgctgtttat ctcctctcgc cgtaatggcg catctcttgg tgagatgatc aaagaagaga
     1981 tggggccagt accggggact atcgcgctgt ttggctgttt cttaatcatg atcatcatcc
     2041 tcgccgtcct ggcgctgatt gtggttaaag ccctggccga aagtccgtgg ggtgtcttca
     2101 ccgtttgctc aaccgtaccg attgcgctgt ttatgggtat ctacatgcgc tttattcgtc
     2161 cggggcgtgt gggtgaagtc tctgtcattg gtatcgtgct gctggttgcc tctatctact
     2221 tcggtggcgt gattgctcac gatccgtact ggggtccggc actgaccttt aaagacacca
     2281 ccattacctt cgcgctgatt ggctatgcgt ttgtttccgc actgctgcca gtgtggctga
     2341 tcctcgcacc acgcgactat ctggcaacct tcctgaaaat cggcgttatc gtcggcctgg
     2401 cgctgggtat cgtggtgttg aacccggaac tgaaaatgcc tgcgatgacc cagtacattg
     2461 acggtactgg cccgctgtgg aaaggcgctc tgttcccgtt cctgttcatc accatcgcct
     2521 gtggtgcggt atctggcttc cacgcgctga tctcttccgg tacgacgcca aaactgctgg
     2581 ctaacgaaac cgacgcgcgt ttcattggct acggcgcaat gctgatggag tccttcgtgg
     2641 cgattatggc gctggttgct gcatccatca tcgaaccggg tctttacttc gcgatgaaca
     2701 ccccgcctgc tggccttggc atcaccatgc ctaacctgca tgaaatgggt ggcgagaacg
     2761 cgccgatcat catggcacag ctgaaagacg ttaccgcaca cgcggcagcg accgtcagct
     2821 cctggggctt cgtgatttcg ccagagcaga tcctgcaaac cgcgaaagac attggtgagc
     2881 cttctgtcct gaaccgtgca ggtggtgcgc caacgctggc ggtaggtatc gctcacgtgt
     2941 tccacaaagt gctgccgatg gctgacatgg gcttctggta tcacttcggt attctgttcg
     3001 aagccctgtt catcctgacc gcgctggatg ccggtacccg ttctggccgc tttatgctgc
     3061 aagacctgct gggtaacttc atcccgttcc tgaaaaaaac cgactctctg gttgccggta
     3121 tcatcggtac tgcgggttgt gtggggctgt ggggctacct gctgtatcag ggcgtggtcg
     3181 atccgctggg cggcgttaag agcctgtggc cgctgttcgg tatctccaac cagatgctgg
     3241 cagccgtagc actggtacta ggcaccgttg tgctgattaa gatgaagcgc acccaataca
     3301 tctgggtaac tgttgttccg gctgtatggc tgcttatctg caccacctgg gcgctgggcc
     3361 tgaaactgtt cagcaccaac ccgcagatgg aaggcttctt ctacatggca agccagtaca
     3421 aagagaagat tgctaacggt actgacctga cggcgcagca gattgccaac atgaaccaca
     3481 tcgttgtgaa caactacacc aacgcaggtc tgagtattct gttcctgatt gtggtgtaca
     3541 gcatcatctt ctacggtttc aaaacctggc ttgcggtgcg taacagcgac aaacgtactg
     3601 acaaagaaac accgtacgtt ccaatcccgg aaggcggcgt gaagatctct tcgcaccact
     3661 aaccgtgttt agccccgctt cggcggggct ttgttcttat cagagtgaac tatgtttggt
     3721 aacttaggac aggcaaaaaa atatctcggc caggcggcga agatgttgat tggcattcca
     3781 gactacgaca actacgtcga gcatatgaag accaaccatc ccgacaagcc gtacatgagc
     3841 tatgaagaat tcttccgcga acgccagaac gcgcgctacg gcggcgatgg taaaggcggt
     3901 atgcgctgtt gttaatggag agagcatgaa cccgattgca gttaccctac tcaccggttt
     3961 tttaggcgcg ggcaaaacca ccctgctgcg ccatattctt aacgaacaac acggctacaa
     4021 gattgccgtg attgaaaacg aattcggcga agtctccgtt gatgatcaac tgattggcga
     4081 ccgcgccacg cagatcaaaa cgctgaccaa cggctgcatc tgctgttcgc gctccaacga
     4141 gctggaggac gcgctactcg acctgctgga taatctcgac aagggcaata ttcagttcga
     4201 ccgtctggtg attgaatgca ccggcatggc cgatcccggc ccgattattc agaccttttt
     4261 ctcccatgag attttatgcc agcgttacct gctggatggc gtgattgcgc tggtggatgc
     4321 ggtacatgcc gatgagcaga tgaaccagtt caccatcgcc cagtcccagg ttggctacgc
     4381 cgaccgtatt ctgctgacta aaaccgacgt cgcaggcgaa gcagaaaaac tgcgtgaacg
     4441 cctggcgcgc atcaacgccc gcgcaccggt ctacaccgtc acccacggcg acatcgacct
     4501 gggcctgctg ttcaacacca acggttttat gctggaagaa aacgtcgtca gcaccaaacc
     4561 gcgtttccac tttatcgcgg ataaacaaaa cgatatttcg tcgattgtgg tggaactgga
     4621 ttacccggta gatatcagcg aagtttcccg cgtgatggaa aacctgctgc tggagtcggc
     4681 ggataaacta ctgcgttaca aagggatgct atggattgac ggcgaaccta atcgcctgct
     4741 gttccagggc gtccaacgcc tctacagcgc cgactgggac aggccgtggg gcgatgaaaa
     4801 accgcatagc acgatggtgt ttatcgggat tcagctgccg gaggatgaga ttcgggcggc
     4861 gtttgcgggg ttgaggaagt aatttgggat gatggccgcc atgtggcggc ctgaattatt
     4921 ttttattctg atcgcgcagc atcgcttcac gtaaaattgc gttcaggcga gtttgatagc
     4981 ctttacctgg tcgcttcaac cattccatca catcagcatc aatacgcacc gaagcctgag
     5041 ttttcaatgg ccgataaaac ttaccgcgtt ctgcgttaga ccattgctca tcgctggaag
     5101 gtgggatatc gctgtaatca atctcttcgt cggatttatt cgccagcacc ttcaattcgg
     5161 cctcatgctg cacatcgagt gtagaggagg tccctcgttt atgtttaacc atgctcatag
     5221 cggctccgtt cgttgtggat catcaaacac taacaccgca tcttcaaagc ggatgccatg
     5281 tttcacccga ttacttttgg ccttgtttgc atcccattca aactccattg gcatagttgc
     5341 catccctaat tgtatataca aaaatgtatc tacaattatt ctgcaattat agcgttgatg
     5401 ttggaaatag catcacgttt cgaataatca accatgaaat tgacaatgtg tgagttggac
     5461 ttgattcatc caatagaatt tctcgcctga catggcacac taaacacctc gctgtcgtta
     5521 agggattacc gtgaaaaact acaaagtcat taccccgctt tttcctacct atgcgcaagt
     5581 taaggctatg atgaaagccg tctcaggcta ttccctgaaa gcggtacgca atatgattac
     5641 cgccattcac gagcaaaccg gaacgccgca aaaacccgtt gactggtcgg aacctgacct
     5701 gtggatcagc gaacgcttaa cgggtgaaga tgccgacatc gcccgtcgta tttgggatac
     5761 cgataatcac atattgaacc cgcgccacag ctacggctgc tacttattcc tcaactatcc
     5821 ccagtttgag ctgatggaaa gcacgccaga tgatacctgg cagcccacct cgcgcgggca
     5881 aaaattcctg caagatgacg aaaaaacgct gcgttcactg gacgaccagg aaggcattct
     5941 gcaactgctt gagctgctgg ctggccggga aatgtcgcgc cgcgccgatt tattgccgga
     6001 atggcaggcc tttttgcatc agcattcgaa attcgcgtca gcaagttcag tgaaaagcac
     6061 gctctactcc cgtctctata accttatcga ccgtgacatg gttaaccgtg aaggcatgag
     6121 ctatcgcatc accgataccg ggcgcgcctg gcttggaaaa gcgttgccag cacgaaaaag
     6181 cgatccgcgt aaagagctgc tggaagcagt gaaacgctac aacgagcagc aaaaagagct
     6241 gctgcgcgaa caaatcagca cgatgaaccc gtacaaattt gagcaactgg ttggtcaatt
     6301 actggaagca atgggctacg agcaggtcga agtgaccaaa gcctcaggcg ataaaggtgt
     6361 ggacgtgatt ggtcaggtgc aggtcggcat caccactatt accgaagtgg tgcaggtcaa
     6421 aagaatgcag aacactatca ctcgcccgta tatcgaccag ttgcgcgggg cgttgcctta
     6481 ccacaaagcc attcgcggta cgctcatcac caccgggaaa tttgcggcga aatgtgcaga
     6541 agcggcgctc tttccgggtg ccgcgcccat tactcttatc gacggcgatc gtttactgga
     6601 actgttaatc gaaaataacg tcggcattcg ccgcagcaat gcagtggaat tgctggatgt
     6661 agatttgcag ttgtttgatg agctggagat ggattaaagg gcgaaatccg ccgcctcaac
     6721 cattacaata gcgacccaat ttatctgaac agagttcatc atggccaaag caccaactaa
     6781 aaaagccaaa gcaaagaaag gctttgaaga aacattatgg gataccgcaa accagcttcg
     6841 cggtagcgtt gagtcctccg aatacaagca cgtggtgttg agcctcgtgt tcctgaaatt
     6901 catcagcgat aagtttgaag cacgccgcaa aaaaatgatt gccgatgggc aagcagactt
     6961 ccttgagatg gaagtgttct accagcagga caacattttc tacctgccgg aagaggcgcg
     7021 ctggtcattt atcaaacaaa atgccaaaca ggacgatatt gcggtccgta ttgacaccgc
     7081 cctctcgacc attgagaaac gtaacccaac gctgaaaggc gcgctgccag acaactactt
     7141 cagccgtcag aatctggaaa ccaaaaaact ggcgtcactg attgatacca tcgacaacat
     7201 cgaaacgctg gcacacgaaa ctgacgttga aacgttatcg aaagaagacc tggtcggacg
     7261 cgtttacgaa tacttcctcg gtaagtttgc cgcaactgaa ggcaaaggcg gtggtgagtt
     7321 ctacacgcct aaatgtgtgg ttacgctgtt aactgaaatg ctcgaaccct tccagggcaa
     7381 aatttatgat ccgtgctgcg gctcggcggg gatgtttgtg cagtcggtga agtttgtcga
     7441 gagccatcag ggtaaaagtc gtgatatcgc gctgtatggt caggaactga cagccacgac
     7501 gtataaactg gcaaaaatga acctcgccat tcgcggtctt tcagctaacc tcggcgaacg
     7561 cccggcaaac actttcttta gcgaccagca cccggacctg aaagccgact acattctggc
     7621 gaacccgccg ttcaacctga aagactggcg taacgaagct gaattaacca aagacccacg
     7681 ttttgccggt tatcgtatgc cgccaaccgg taacgccaac tacggctgga ttttgcatat
     7741 gctctccaag ctgtcggcta acggtacagc aggttttgtg ctggcaaacg gttcgatgag
     7801 ttctaacacc agcggtgaag gcgagatccg tgcgcagatg atcgaaaatg atctgatcga
     7861 ctgcatgatc gccctacctg gacagttgtt ctacaccacg cagatcccgg tgtgtttatg
     7921 gtttatgacc aaatcgaagg ctgccgaccc agccaaaggt tatcgtgatc gtcagggcga
     7981 gacgctgttt attgatgcgc gtaacctcgg caccatgatt agccgcacaa ctaaagagtt
     8041 aacgacggaa gatattgcca caatcgccga tacctaccat gcctggcgca gcacgccgga
     8101 agaactggct gcacggattg cgcgtggtga cagcaagttg gaaaaatatg aagaccaggc
     8161 aggtttctgc aaagttgcaa ccctgcaaga tattaaagat aacgactacg tactgacacc
     8221 gggccgctat gtgggtgcag ccgagcagga agaagacggc gtggcatttg agacgaaaat
     8281 gcgcgaattg tcgaagacgt tgtttgagca gatgaagcag gcggaagaac tggatcgtgc
     8341 gattcgccag aatctggagg cgctgggtta tgggctttga ttttcagttg ttagaaaatg
     8401 cagggatcca gctaattgat ggtgacagag gcaaaaatta tcctaaacat aatgatttta
     8461 tggagaatgg ctattgtctt ttcttatccg ctaaaaatgt cactaaatct ggttttcaat
     8521 ttcaagaaac attatttatc aatgaaacta aagatcgtga actaagagca ggaaagctta
     8581 agtacggtga cattgttctt act
//
LOCUS       DAPHCV010000160         8592 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.162, whole genome shotgun sequence.
ACCESSION   DAPHCV010000160 DAPHCV010000000
VERSION     DAPHCV010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.162"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(323..529)
                     /locus_tag="RH033_004233"
     CDS             complement(323..529)
                     /locus_tag="RH033_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HDV2993330.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSEKPVWMDQAEKATGDFY"
     gene            842..931
                     /gene="kdpF"
                     /locus_tag="RH033_004234"
     CDS             842..931
                     /gene="kdpF"
                     /locus_tag="RH033_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDV2993331.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            931..2604
                     /gene="kdpA"
                     /locus_tag="RH033_004235"
     CDS             931..2604
                     /gene="kdpA"
                     /locus_tag="RH033_004235"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HDV2993332.1"
                     /translation="MAAQGFLLIATFLLVFMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFSALGVSDREMNWKQYLSAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVAGD
                     RRQGRMLLWAMSVIFVICTSVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLALCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2627..4675
                     /gene="kdpB"
                     /locus_tag="RH033_004236"
     CDS             2627..4675
                     /gene="kdpB"
                     /locus_tag="RH033_004236"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HDV2993333.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTFICIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSHVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIAFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4684..5256
                     /gene="kdpC"
                     /locus_tag="RH033_004237"
     CDS             4684..5256
                     /gene="kdpC"
                     /locus_tag="RH033_004237"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDV2993334.1"
                     /translation="MRGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5249..7933
                     /gene="kdpD"
                     /locus_tag="RH033_004238"
     CDS             5249..7933
                     /gene="kdpD"
                     /locus_tag="RH033_004238"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HDV2993335.1"
                     /translation="MNNEPLRPDPDRLLEQAAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVAGVVETHGRKDTAAMLEGLAVLPLKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSSQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGEKLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7930..>8592
                     /gene="kdpE"
                     /locus_tag="RH033_004239"
     CDS             7930..>8592
                     /gene="kdpE"
                     /locus_tag="RH033_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HDV2993336.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGY"
BASE COUNT         1826 a         2215 c         2496 g         2055 t
ORIGIN      
        1 tttttattgc ggtagcgata ttaatagcaa aattcggaat ttatttagat gctggaataa
       61 gaaaaagtga tggcggcttt ctcaccttac cctgaagttt caggaaagtg tgtaaggaag
      121 gatgcaacct aactggagat ttctgaggat ctagttgctt agagcgacaa tgaaggtgaa
      181 cgcatccaga gtagtcctat gaaaggatac tattgaaaat tgatgctaaa acagtgatta
      241 aatgaacaac accatggaac ccgttgattc aaaacgccta aaccagcccc ctgatacgag
      301 gctggcttaa acgcgcaggc aatcagtaaa aatcacccgt tgccttttcg gcctgatcca
      361 tccacaccgg tttctcgctg gttttcgacc agacgcggtg caggtagctg taaaagcggg
      421 cgcggtcttt ccagaaaagc atgaaaggca gcgccagaac gcccgctgca accgcataag
      481 tacggcgtaa aaagataagc catgcaggat attctctgta gagttccata cttttctccc
      541 ccttttgtat ctacccggtg aatggcaccg gaaaaatgaa tttgtttatc tgatgaaaat
      601 agtaccgcct tttgtgtaat tttactactc atccgaccac ttatttttgc ttattgatgg
      661 tttatttaca ttcacactgt aattaagtta cacaaaagtt aaattaatac taaccatcag
      721 ttaaatcatg gcttttgcca tttttatact ttttttacac cacgcccgaa gatttttgcg
      781 aaatctttgc agccagaatt ctacccttcc ggtatcactt ttaggccact ggaggtgcac
      841 tgtgagtgca ggcgtgatag ccggcgtgtt gctggtgttt ttattactgg gttatctggt
      901 ttatgccctg atcaatgcgg aggcgttctg atggctgcgc aagggttctt actgatcgcc
      961 acgtttttac tggtgttcat ggtgctggca cgtcctttag gcagcgggct ggcgcggctg
     1021 attaatgata ttcctcttcc tggtacgacg ggcgttgaac gcgtactctt tagcgcgctt
     1081 ggcgtctctg accgcgagat gaactggaag cagtatctct cagccattct tggcctgaat
     1141 atgctggggc tggcggtgct gttttttatg ttgctcggtc agcactatct gccgcttaat
     1201 ccacagcagt tgccagggct gtcatgggat ctggcgctga ataccgccgt cagctttgtc
     1261 accaatacca actggcaatc ttatagcggt gaaaccacgt tgagctattt cagccagatg
     1321 gcgggcttaa cggtgcaaaa ctttctttct gccgccagcg ggattgcggt gatttttgcc
     1381 ctcatccgtg cgtttacccg ccagagcatg agcacactcg ggaatgcctg ggtcgatctg
     1441 ctacgcatca cgttatgggt gctagtccct gtggcgttgt tgatcgcact gttttttatt
     1501 caacaaggtg cgctgcaaaa ctttctgccc tatcaggcgg tgaataccgt tgaaggagcg
     1561 caacagctgt tacccatggg gcctgtagct tctcaggaag cgatcaagat gctcggtact
     1621 aacggcggtg gcttctttaa tgccaactcg tcgcatccgt ttgaaaaccc aaccgcactg
     1681 accaacttcg tgcagatgct agcgatcttc ttgatcccaa cagcgctgtg ctttgccttt
     1741 ggtgaagtgg cgggcgatcg tcgtcagggg cgcatgttgc tgtgggcgat gtcagtgatt
     1801 tttgttatct gcacaagcgt ggtgatgtgg gcagaagttc agggtaatcc tcatctgctg
     1861 gcgctgggtg cggacagcag catcaatatg gaaggtaaag agagccgttt cggcgtgctg
     1921 gtcagtagcc tgtttgcggt cgtgactacg gcggcttcct gtggcgcggt gattgcgatg
     1981 catgattcgt ttaccgctct tggtggcatg gtgccgatgt ggttgatgca aattggtgaa
     2041 gtggtattcg gcggcgtcgg ttcaggcctt tacggcatga tgctgtttgt cctgctggcg
     2101 gtgtttattg ccgggctgat gattggtcgt acaccggaat atctgggtaa aaaaatcgac
     2161 gtacgcgaga tgaaactgac tgcactggcg attctggtca ccccgacgct ggtgctgatg
     2221 ggcgcggcgc tggcgatgat gaccgacgcc ggacgtagcg ccatgctcaa ccctggcccg
     2281 catggtttta gcgaagtgct gtacgccgtg tcatccgccg ctaacaacaa cggcagcgcc
     2341 tttgccggat taagcgccaa ctctccgttc tggaactgtt tactggcgtt gtgcatgttt
     2401 gtcgggcgct tcggggtgat tatcccggtg atggcaattg ccggttcgct ggtgagtaaa
     2461 aagagccaac ccgccagctc cggcacgctg ccaacgcacg gcccgctgtt tgttggcctg
     2521 ttaatcggca ccgtgttgct ggttggcgca ctgaccttta tccctgccct ggcgcttggt
     2581 ccggtggcgg aatatctctc ctgatgatat tgagtgagca ctgaatatga gtcgtaaaca
     2641 actggcgcta ttcgaaccaa cacttgtcgt tcaggcgctg aaagaagcgg tgaaaaaatt
     2701 aaacccgcag gcgcaatggc gtaatccggt gatgtttatc gtctggatcg gcagtctgtt
     2761 aaccaccttt atttgcatcg caatggcaag cggtgcgatg cctggcaatg cgctgtttag
     2821 cgcggcaatt agcggctggc tgtgggtcac cgtactgttc gctaatttcg ccgaggcgct
     2881 ggcagagggc cgcagtaaag cgcaggccaa cagtctgaaa ggggtgaaaa aaactgcctt
     2941 tgcccgcaag cttcgtgagc cgaaatatgg cgctgcggca gacaaagttc ctgccgatca
     3001 actgcgtaaa ggcgatatcg tactggtaga agctggcgat attatcccct gcgatggtga
     3061 agttattgaa gggggtgcat cggtcgatga aagcgccatc accggggaat cagcaccggt
     3121 tatccgtgaa tccggcggcg attttgcctc cgtcaccggc ggcacgcgta ttctttctga
     3181 ctggctggta attgagtgca gcgttaaccc cggtgagaca tttctggatc ggatgatcgc
     3241 gatggtggaa ggcgcacagc gacgcaaaac gccgaacgag attgccctga ccattctcct
     3301 gattgccctg actatcgtct ttttactggc aaccgccacg ctgtggccgt tttccgcgtg
     3361 gggcggtaat gcagtgagtg taacggtact ggtggcgctg ctggtctgtc tgatcccaac
     3421 cactattggc ggcctgttgt cagcgatcgg cgtcgccggg atgagtcgga tgctgggcgc
     3481 gaatgtgatt gccaccagcg gacgtgcagt tgaagcggca ggtgacgttg acgttctgct
     3541 actggataaa accggcacca tcacactcgg taaccgtcag gcgtcggagt ttatccccgc
     3601 gcagggcgtg gatgaaaaaa cgctggctga cgccgcacaa ctggcttcgc tggctgatga
     3661 aacgccggaa ggtcgcagta ttgtgatcct cgccaagcaa cgttttaacc tgcgcgagcg
     3721 cgatgtgcag tcgctccatg ccacctttgt accgtttact gcgcaaagcc ggatgagcgg
     3781 gatcaacatc gacaaccgca tgatccgtaa aggttctgtc gatgccattc gccgccatgt
     3841 tgaggctaat ggtggtcact tccctgccga tgtcgatcaa aaagtcgatc aggttgcgcg
     3901 tcagggagcc acgccgctgg tagtagtaga aggctcccat gtgcttggcg ttattgcgct
     3961 gaaagatatc gtcaaaggcg gtattaaaga gcgcttcgcg cagttgcgta aaatgggcat
     4021 taaaacggtg atgattaccg gcgataaccg tctgactgcc gccgcgatag ctgcggaagc
     4081 gggcgtcgat gattttctcg ccgaagcaac accggaggcc aagctggcac tgattcgtca
     4141 gtatcaggcg gaaggccgtt tagtggcgat gaccggcgat ggcaccaacg atgctccggc
     4201 gctggcacag gccgatgttg cggtggcgat gaactccggc acccaggcgg cgaaagaagc
     4261 gggcaatatg gtggatctcg actccaaccc gaccaagttg atcgaggtag tgcacattgg
     4321 caaacagatg ctgatgaccc gtggctcgtt gaccaccttc agcattgcca acgatgtggc
     4381 gaaatacttc gccattattc cagcggcgtt cgcggcaacg tatccgcagt taaatgcgct
     4441 gaacatcatg cgcctgcatt cgcccgactc cgccattctc agtgcggtca ttttcaacgc
     4501 cttgattatc gcctttttga ttcccctggc gttaaaaggc gtgagttata aaccgcttac
     4561 cgcttctgcc atgttgcgcc gtaacttatg gatttacggt ctgggtgggc tgctggtgcc
     4621 gtttattggt atcaaagtca ttgatttact gctaaccgtt tgcggtctgg tgtgaggttt
     4681 accatgcgtg gattacgtcc ggcattatcg acatttctct ttctgttatt gattactggc
     4741 ggcgtttacc cgctgctgac caccgcactg gggcaatggt ggtttccctg gcaggccaac
     4801 ggttcattga ttcgtgaagg tgatacggtg cgcggttcgg cattaatcgg gcaaaatttt
     4861 accggcaacg gctattttca tggtcgcccg tcggcaacag cagaaatgcc ctataatcca
     4921 caggcttctg gcgggagcaa tctggcagtc agtaacccgg agctggataa acagatagcc
     4981 gcacgcgttg ccgcattacg ggctgctaac ccggatgcca gcacgaacgt tccggttgaa
     5041 ctggtgacgg cgtcagcaag cgggctggac aataacatca ccccgcaagc ggcggcctgg
     5101 cagatcccac gcgtggcgaa agcgcgtaat ctcagcgttg aacagctcac gcaactgatc
     5161 gcaaaataca gccaacaacc gctggtgaaa tatatcggcc agccggtcgt caacattgtc
     5221 gaactcaatc tggcactgga taagcttgat gaataacgaa cccttacgtc ccgaccccga
     5281 tcgtctgctg gaacaagctg ccgcgccgca tcgggggaag ctgaaagtat tcttcggtgc
     5341 ctgcgcaggc gtcgggaaga cctgggcgat gctggcggaa gcccagcgac tgcgggcgca
     5401 agggctggat attgtggctg gcgtggtaga aacccacggt cggaaagata ccgccgcaat
     5461 gctggaaggg ctggctgttc tgccgttaaa acgccaggcg taccgtgggc ggcacatcag
     5521 cgagtttgat ctcgatgccg ccctcgcccg ccgtccggca ctgatcttaa tggacgaact
     5581 ggcgcacagt aatgcgccag gttctcgtca tcccaaacgc tggcaggaca tcgaagagct
     5641 gctggaagct ggcattgatg ttttcactac cgtcaacgtt caacatctgg aaagcctgaa
     5701 tgatgtggtc agcggcgtca ccggaattca ggttcgggaa accgtgcccg atcctttttt
     5761 tgatgccgcc gacgacgtag tgctggtgga cttgcccccg gacgatctgc gccagcgact
     5821 gaaagaaggc aaagtctata ttgccgggca ggcggagcgc gccattgaac attttttccg
     5881 caaaggtaat ctgatcgccc tgcgcgaact ggcactacgc cgtactgccg atcgcgtaga
     5941 tgagcaaatg cgcgcctggc gggggcatcc tggcgaagag aaagtctggc acacacgcga
     6001 cgcgatccta ttatgcatcg ggcataacac cggcagtgag aaactggtcc gcgcggcggc
     6061 gcggttggcc tcgcgcctgg gcagtgtctg gcacgcggtg tatgttgaaa cccctgccct
     6121 gcatcgctta ccggagaaaa aacgtcgggc aattctcagc gccttacgtc tggcgcagga
     6181 gctgggcgcg gagacggcaa cactttctga tccagcggaa gagaaagcgg tagtgcgtta
     6241 tgcccgtgaa cataatctcg gcaagattat tctcggtcgc ccggcttcgc gccgctggtg
     6301 gcgtcgggaa acatttgctg accggctggc acgcatcgcc cccgatctcg atcaggtgct
     6361 ggtcgcgctt gatgaaccac ccgcccgcac gattaacaac gcgccggata gccgctcttt
     6421 taaagacaag tggcgggtac agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt
     6481 tatcacctta attgccatgc agtggctgat ggcgtttgat gccgccaacc tggtgatgct
     6541 gtatctactt ggcgtggtgg tggtggcgct attttatgga cgctggcctt cggtggttgc
     6601 taccgtcatt aatgtggtga gtttcgatct cttttttatc gccccacgcg gcacgctcgc
     6661 cgtctctgat gtgcaatatc tgctgacctt cgcggtgatg ttaaccgtcg ggctggtgat
     6721 cgggaacctt actgctggcg tgcgttatca ggcgcgggta gcccgttacc gcgaacaacg
     6781 cacacggcac ttatatgaaa tgtccaaagc tctggctgtg ggccgtagtt cgcaggatat
     6841 cgccgccacc agcgaacagt ttattgcctc cacgtttcat gcccgtagcc aggtgttgct
     6901 gcctgatgac aacggtaaat tgcagccgtt aacacatccg caaggaatga cgccgtggga
     6961 cgatgccatc gcgcagtgga gttttgataa aggtctgcct gcgggcgcgg gcaccgacac
     7021 gttacccggt gtaccgtacc agattttgcc gctaaaaagc ggcgagaaaa cctacggact
     7081 ggtggtggtg gaaccgggga atctgcgcca gttgatgatc ccggaacagc agcgcctgct
     7141 ggagacgttt acgctgttag tcgccaatgc gcttgagcgg ctgacgctga ccgccagcga
     7201 agaacaggcg cggatggcaa gcgaacgtga acagatccgc aacgctctgc tggcggcgct
     7261 ttcgcatgat ttacgcacgc cgctcacggt gctgttcggt caggcagaaa tcttaacgct
     7321 cgatctggca agcgaaggat cgccccacgc ccgccaggcc agcgagattc gtcagcatgt
     7381 gctgaacacg acccgactgg tgaataatct gctcgatatg gcgcgaattc agtccggcgg
     7441 ctttaatttg aagaaagagt ggttaacgct ggaagaagtg gtcggcagcg cgctgcaaat
     7501 gctggaaccg ggtttatcgt cgcctatcaa tctttctctg ccagaaccgc tgaccttaat
     7561 ccacgttgac gggccgctct ttgaacgggt gctgattaat ctgctggaga acgcggtgaa
     7621 atacgcgggt gcgcaggccg aaattggtat cgatgcccac gttgagggcg agaagctaca
     7681 actggatgtc tgggataacg gccccggtct tccgccaggc caggagcaga cgatatttga
     7741 taagtttgct cgcgggaata aagagtcggc ggtaccgggt gtagggcttg ggctggcaat
     7801 ttgtcgggcg attgtggatg tacacggcgg caccattacc gcgttcaacc gaccggaagg
     7861 tggtgcctgt tttcgtgtta cacttcccca gcaaaccgct cctgaacttg aagaatttca
     7921 tgaggatatg tgacaaacgt tctgattgtt gaagatgaac aggctattcg tcgctttctg
     7981 cgcacggcgc tggaaggcga cggtatgcgc gtctttgagg ccgaaacgct gcaacgcggc
     8041 ttgctggaag cggctacccg taagccagat ttgattattc tcgatctcgg cctgcccgat
     8101 ggtgatggga ttgagtttat ccgcgacctg cgccagtgga gcgcggtgcc ggtgattgtg
     8161 ctttccgcac gtagcgaaga gagcgacaaa atcgccgcgc tggatgccgg agcggatgat
     8221 tatctgagta agccgtttgg cattggcgaa ttgcaggccc gtctgcgcgt cgctttacgt
     8281 cgccactccg ccaccgccgc gcccgatccg ctggtgaaat tttccgatgt taccgtcgat
     8341 ttagcagctc gtgtgatcca tcggggcgag gaagaggtgc atctcacccc gattgagttc
     8401 cgcctgctgg cagtgttgct taataatgcg ggaaaagtgc tcacccagcg ccagctcctt
     8461 aaccaggtgt gggggccaaa cgcggtcgaa cacagtcatt atttgcgtat ttatatgggg
     8521 catctgcgac aaaaactgga acaggacccg gcccggcctc gccattttat tactgaaacc
     8581 ggtattgggt at
//
LOCUS       DAPHCV010000161         8434 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.163, whole genome shotgun sequence.
ACCESSION   DAPHCV010000161 DAPHCV010000000
VERSION     DAPHCV010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.163"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..351
                     /locus_tag="RH033_004240"
     CDS             <1..351
                     /locus_tag="RH033_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDV2993337.1"
                     /translation="AYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYD
                     LSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHP
                     KLQGIAQQNSFKHT"
     gene            507..2042
                     /gene="gadC"
                     /locus_tag="RH033_004241"
     CDS             507..2042
                     /gene="gadC"
                     /locus_tag="RH033_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HDV2993338.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSTALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKSKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2173..3492
                     /locus_tag="RH033_004242"
     CDS             2173..3492
                     /locus_tag="RH033_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HDV2993339.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPTGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMVDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPEVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3789..3830
                     /locus_tag="RH033_004243"
     CDS             3789..3830
                     /locus_tag="RH033_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993340.1"
                     /translation="MVQLVDLAHCVSF"
     gene            3868..5250
                     /gene="dosC"
                     /locus_tag="RH033_004244"
                     /pseudo
     CDS             3868..5250
                     /gene="dosC"
                     /locus_tag="RH033_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
     gene            5275..7674
                     /gene="dosP"
                     /locus_tag="RH033_004245"
                     /gene_synonym="pdeO"
     CDS             5275..7674
                     /gene="dosP"
                     /locus_tag="RH033_004245"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HDV2993341.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPQYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCINEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGTPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWTDQALLEVVNRFREKLKPDQYLCRI
                     EGASFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDEGKNRDYL
                     LSTAHNAMDFIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWYDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGIGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGIETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            complement(7864..8295)
                     /gene="osmC"
                     /locus_tag="RH033_004246"
     CDS             complement(7864..8295)
                     /gene="osmC"
                     /locus_tag="RH033_004246"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HDV2993342.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2174 a         2040 c         2033 g         2187 t
ORIGIN      
        1 gcttatctgg cggatgaaat cgccaaactg gggccgtatg agttcatctg tacgggtcgc
       61 ccggacgaag gcatcccggc ggtttgcttc aaactgaaag atggtgaaga tccgggatac
      121 acactctacg acctctctga acgtctgcgt ctgcgcggct ggcaggttcc ggccttcact
      181 ctcggcggtg aagccaccga catcgtggtg atgcgcatta tgtgccgtcg cggcttcgaa
      241 atggactttg ctgaactgtt gctggaagac tacaaagcct ccctgaaata tctcagcgac
      301 cacccgaaac tgcagggtat tgcccagcag aacagcttta aacacacctg ataacgttta
      361 acggtaacgg tgtcccgaaa cgaagccgtt tcgggacaat ttccaaagtc tgttcactgg
      421 cattagcaac ggaaaatatt gttctgaata cgcttcagaa caaaacaggt gcggttccga
      481 caggaatacc gttttagggg gataatatgg ctacatcagt acagacaggt aaagctaagc
      541 agctcacatt actcggattt tttgccataa cggcatcgat ggtaatggct gtttatgaat
      601 accctacctt cgcaacatcg ggcttttcat tagtcttctt cctgctatta ggcgggattt
      661 tatggtttat tcccgtggga ctttgtgctg cggaaatggc taccgtcgac ggctgggaag
      721 aaggtggcgt cttcgcctgg gtatcaaata ctctggggcc gagatgggga tttgcagcga
      781 tctcatttgg ctatctgcaa atcgccattg gttttattcc gatgctctat ttcgtgttag
      841 gggcactctc ctacatcctg aaatggccag cgctgaatga agaccccatt accaaaacta
      901 ttgcagcact catcattctt tgggcgctgg cattaacgca gtttggtggc acgaaataca
      961 cggcgcgaat tgctaaagtt ggcttcttcg ccggtatcct gttacctgca tttattttga
     1021 tcgcattagc ggctatttat ctgcactccg gtgcccccgt tgctatcgaa atggattcga
     1081 agaccttctt ccctgacttc tctaaagtgg gcaccctggt tgtatttgtt gccttcattt
     1141 tgagttatat gggcgttgaa gcttccgcaa cccacgtcaa tgaaatgagc aatccagggc
     1201 gcgactatcc actggctatg ttactgctga tggtggcggc aatctgctta agctctgttg
     1261 gcggtttgtc tattgcgatg gtcattccgg gtaatgaaat caacctctcc gcaggggtaa
     1321 tgcaaacctt taccgttctg atgtcacatg tggcaccgga aattgagtgg acggttcgcg
     1381 tgatctccgc actgctgttg ctgggtgttc tggcggaaat cgcttcctgg attgttggtc
     1441 cttctcgcgg gatgtatgtc acagcgcaga aaaacctgct gccagcggca ttcgctaaaa
     1501 tgaacaaaaa tggcgtaccg gtaacgctgg tcatttcgca gctggtgatt acttctaccg
     1561 cgttgatcat cctcaccaat accggtggcg gtaacaacat gtccttcctg atcgcactgg
     1621 cgctgacggt ggtgatttat ctgtgtgctt atttcatgct gtttattggc tacattgtgt
     1681 tggttcttaa acatcctgac ttaaaacgca catttaatat ccctggtggt aaaggggtga
     1741 aactggtcgt ggctattgtc ggtctgctga cttcaattat ggcgtttatt gtttccttcc
     1801 tgccgccgga taacatccag ggtgactcta ccgatatgta tgttgaatta ctggtcgtta
     1861 gtttcctggt ggtacttgcc ctgcccttta ttctctatgc tgttcatgat cgtaaaagca
     1921 aagcgaatac cggcgtcact ctggagccaa tcaacagtca gaacgcacca aaaggtcact
     1981 tcttcctgca cccgcgtgca cgttcaccac actatattgt gatgaatgac aagaaacact
     2041 aaccatgtct gaatggttat aagataaggg agcgataatt catcgctccc tttttcgtgc
     2101 ttgctgtcgt attgacttaa ccgggataag tacgagaatg agcgcacatc tgtttaccgg
     2161 aaaccagcac atatggatat ctgctcccga aacgagaaat tagcgattag aagaccagcg
     2221 atactggttg cactggcact tttactgtgt agttgcaaaa gcacgcctcc agagtcaatg
     2281 gtgacaccac caacaggttc gaagccacca gctacgacgc aacaatcgtc acaaccgatg
     2341 cgtggcatct ggctggccac agtgtctcgc ctcgactggc caccggtttc ctcggttaac
     2401 attagtaacc ccaccagccg ggcccgtgta caacaacagg cgatggtcga caaactggat
     2461 catctgcaac gtctcggcat caacacggtc tttttccagg tcaagccgga cggtaccgcc
     2521 ctgtggccat cgaaaatttt gccgtggtcc gatcttatga ccggaaagat tggtgaaaat
     2581 ccaggttacg atccgctgca atttatgctc gacgaagccc acaagcgtgg gatgaaagta
     2641 cacgcctggt ttaaccccta tcgcgtatcg gttaatacga agcccggtac tatcagggaa
     2701 ctgaatagca ctctgtctca acaaccagcg agcgtctatg tgcaacaccg cgactggatc
     2761 agaacgtccg gcgatcgctt tgtcctcgac ccgggcatac ctgaggttca ggactggatc
     2821 acatcaatag tcgcggaagt ggtttcccgc tatccggtag atggcgtgca gtttgacgac
     2881 tatttctata ctgagtcacc aggttcacgg ctaaatgata acgaaacgta ccgcaaatac
     2941 ggaggcgcat ttgcgtcaaa agcagactgg cggcgcaaca atactcagca gttaattgca
     3001 aaggtatcgc acactattaa aagcattaag ccggaagtcg aatttggcgt tagcccggca
     3061 ggcgtgtggc gtaaccgatc acacgatccg ctcggttccg atacccgagg cgcggcagcc
     3121 tatgacgaat cctacgctga cacccgtcga tgggtggaac aaggattgct ggattacatt
     3181 gctccccaaa tttactggcc gttctcacgg agtgccgcac gttatgacgt tttggcaaaa
     3241 tggtgggcgg atgtcgttaa accgaccagg acccgcctgt atatcggtat cgccttctat
     3301 aaagtaggag aaccttcaaa gatagagcca gactggatga ttaacggcgg cgtaccggaa
     3361 ctgaaaaagc agctcgatct taacgatgcc gtgccagaaa ttagcggcac aatcttgttc
     3421 cgtgaggact atctgaataa gccacagact caacaagcgg tcagctatct gcaaagtcgt
     3481 tggggcagtt aaactcccat tcttactttg taacaagccg gtgctttccc cccggcttgt
     3541 taccctcttg aaatatccca ctagtaatca tgcttactta agtcaaatta actacgcaat
     3601 taaccacact tagttaaaca gtcataaatc ccctaataaa aatgtatgaa tatgaaaaaa
     3661 catacgatat tcgctgtcac cgaaaaataa ttacgatggg ctatactctt ctgaagaaca
     3721 gtagcgtaga ttgaaactgt cctaagtcgc ttatttgttt tcagtatatc ttcatatttc
     3781 aggagaatat ggtgcagtta gtcgatctcg cacattgcgt ttcgttttga ttccgtctgc
     3841 tttcccagcc ttataagggt gtgaattatg gagatgtatt ttaaaagaat gaaagatgag
     3901 tggaccgggc ttgtcgaaca agcagatccg ctcattcgtg ctaaagccgc ggaaattgcc
     3961 gttgcgcatg cccattatct gagtattgag ttttaccgaa ttgtccgtat cgacccgcat
     4021 gccgaagaat tcttgagtaa tgaacaagtt gagcggcagt tgaaaagtgc gatggaacgc
     4081 tggattatta acgtgctttc tgcccaggtt gacgatgtcg aaaggctaat acaaatccag
     4141 cataccgttg cggaagtgca tgcccgcata ggaattccgg tagaaattgt cgagatgggg
     4201 tttcgggtgc tgaaaaaaat cctctatcca gtcatcttct cttcggatta ttccgccgca
     4261 gaaaaacttc aggtctacca tttctcgatt aacagtattg atatcgctat ggaagtgatg
     4321 acccgcgcgt ttacctttag tgacaatagt gcctcaaagg aagatgaaaa ctatcgtatc
     4381 ttctcgttac tggaaaatgc cgaagaagaa aaagaacggc aaatagcctc attactttca
     4441 tgggaaatag atattatcta taaagtcctg ctggattctg atttaggcag tagtttgcct
     4501 ttaagccagg ctgattttgg cctgtgattt aaccataaag gtcgacatta ttttagcggc
     4561 atagccgaag tgggccatat ctcccgtctg attcaggatt tcgacggtat tttcaatcaa
     4621 accatgcgta ataccaggaa tttgaataac agaagtttgc gggtgaaatt tttattacag
     4681 ataagaaata ccgtatcgca aattattacc ttgctgcgtg aattgtttga agaagtatcg
     4741 cgccacgaag tcggtatgga tgtcctgacg aaattactta accgccgttt cctgccgacg
     4801 atcttcaaac gcgaaattgc ccatgccaac cggaccggta caccgctgtc agtgctgatt
     4861 attgacgttg ataaattcaa agagatcaac gatacgtggg gccataacac tggcgatgaa
     4921 attctgcgta aagtctctca ggccttttat gacaacgtcc gcagtagtga ttatgttttc
     4981 cgctacgggg gcgatgaatt tatcattgtt ttgactgaag cttcggaaaa cgaaacgtta
     5041 cgtaccgcag aacgtattcg cagtcgggtg gagaaaacca aactgaaagc cgcaaacggc
     5101 gaggatattg ccctctcact ttccatcggt gccgccatgt ttaatggtca tcctgactat
     5161 gagcgcctca ttcaaatagc cgatgaagct ctgtatatcg ccaaaagacg aggtagaaac
     5221 cgtgttgaac tctggaaagc cagtctttag atgcgccagg atgcagaggt aatcatgaag
     5281 ctaaccgatg cggataatgc cgccgatggc atttttttcc ccgcccttga gcaaaatatg
     5341 atgggcgcgg tgttaattaa cgaaaatgat gaagtgatgt ttttcaaccc cgccgcagag
     5401 aagctctggg ggtacaaacg tgaagaagtc attggcaata acattgatat gctgattccg
     5461 cgggatttgc gtcctgcgca tcctcaatac attcgtcaca accgtgaagg tggtaaagct
     5521 cgcgtcgagg ggatgagtcg ggagctgcag ctggagaaaa aagacggcag taaaatctgg
     5581 acccgttttg cgctatcgaa agtgagcgcc gaggggaaag tttattacct ggcgctggta
     5641 cgggatgcca gcgtagaaat ggcgcaaaaa gaacagaccc gacaattgat tattgccgtt
     5701 gaccatctcg accgaccggt gattgtcctc gatccggaac gccatattgt gcagtgcaat
     5761 cgcgcattta ccgaaatgtt tggttactgc attaacgaag ccagcggtat gcagcccgat
     5821 acactcctga acattccaga attccctgct gataaccgta ttcgtttaca gcagttgcta
     5881 tggaaaaccg cccgcgatca ggacgaattt ctgctgttga cgcgcaccgg tgaaaaaatc
     5941 tggattaaag cctctatcag cccggtttat gacgtgctcg cgcatctgca gaacctggta
     6001 atgacattct cggatatcac cgaagaaagg caaatccgcc agcttgaagg caatattctc
     6061 gccgccatgt gcagcagccc cccatttcat gaaatggggg aaatcatttg tcgtaacatc
     6121 gaatctgttc tcaacgaatc gcatgtttcg ctgttcgcgc tgcgcaacgg gatgccgata
     6181 cactgggcgt catcttcgca cggtgcagaa gttcaaaatg cgcaaagctg gtcagcgacc
     6241 attcgtcagc gcgatggcac gcctgcgggg atcctgcaaa ttaaaacctc gtcaggagca
     6301 gaaaccagcg cctttatcga acgcgtggca gatatcagcc agcatatggc cgcgctggcg
     6361 ctggaacagg aaaaaagccg tcagcatatt gaacaactca tccaatttga tccgatgacc
     6421 ggtctgccaa atcgcaataa cctgcacaat tacctcgatg acctggtcga caaagccgtc
     6481 tctccggtgg tgtatctcat cggtgttgac catattcagg atgtgattga tagccttggc
     6541 tatgcgtgga ccgatcaggc attgctggaa gtggtcaatc gctttcgtga aaaactcaaa
     6601 ccggatcagt atctctgtcg tattgaaggt gcgtcttttg tcctcgtgag cctggaaaac
     6661 gatgtcagta acattaccca gatcgccgat gagctacgga atgtggtcag caagccgata
     6721 atgattgacg ataaaccatt cccgctcacc ttgagcattg gcatcagcta cgacgagggt
     6781 aaaaaccgcg attacctgct ctccactgct cacaatgcaa tggattttat tcgcaaaaat
     6841 ggcggtaacg gctggcagtt cttcagcccg gcgatgaacg aaatggtaaa agagcgtttg
     6901 gttttaggcg cagcgctaaa agaagcgatt agtaataacc aacttaaact ggtttatcag
     6961 ccgcaaatct tcgcagaaac gggtgaactg tacggcatcg aagcccttgc tcgctggtac
     7021 gatcccctgc atggtcatgt gcccccttca cggtttattc ctctcgcaga agagattggt
     7081 gaaatcgaaa atattgggcg ctgggtcatc gcggaagctt gccgtcagtt agcagaatgg
     7141 cgtagccaga atattcatat cccggcgtta tccgttaact tgtctgcgct gcactttcgc
     7201 agtaatcagc tgcctaatca ggtatctgat gcaatgcacg cctggggtat tgacggccac
     7261 cagctgacgg tggaaatcac ggaaagcatg atgatggaac acgataccga aatctttaag
     7321 cgcattcaga tcctgcgcga tatgggcatc ggtttatcgg tagatgattt tggtacaggc
     7381 ttttccggat tatcccgctt agtcagtctt ccggtaacgg aaatcaaaat tgataaaagt
     7441 tttgtcgatc gttgtctgac cgaaaaacgc atccttgcct tactcgaagc cattaccagc
     7501 attgggcaaa gcctcaattt aaccgtcgtg gcggaaggca tcgaaaccaa agagcaattt
     7561 gagatgctac gcaagatcca ctgtcgcgtt attcagggat atttcttttc ccgccccctg
     7621 cccgccgaag aaattccagg ctggatgagc agcgtgttac cgctgaaaat ctgataaatt
     7681 cctctcgccc gcactcgcgg gtttttattt aacgtgacac tgtcacttaa atactgtgat
     7741 ttccgctacg tttagttgga caacgacaac gcatagccat gtcacaacaa tcccttctca
     7801 gcctgatgtg attgtgctga cgcatcaggc aatcacgcgc ctgacgcgtc atccggcaac
     7861 actttacgat ttcaactggt aatcaagcgt aatttcagct ttcagtacct gagaaaccgg
     7921 gcatcctgct tttgctttct ggataatgcc gtcaaaagta gaggcatcaa tacccggcac
     7981 cgcaacttcg cttttcagtg cgattttagt aatcgcaaaa ccggcatcca ctttatccag
     8041 cgacacatct gcggtggtat caatcgatgt tggcgtgaat cccgcttccc ccagcattaa
     8101 tgaaagcgcc attgagaaac atgcggcatg cgctgcgcca atcagttctt cagggttggt
     8161 tcctttttcg ccttcaaaac gcgtgttaaa tccatacggc tgttggttca gcacgccgct
     8221 ctcggtggat acagttccct tcccgcgttt gatatcgcct tcccagtgtg cctgaccttt
     8281 cttatggatt gtcattgttg ctctcctgtg ggcttaaaaa ggaaattata gactacgtga
     8341 gaaacagcac gaggataagc aggatattcc gaataaaatc cggcgaaaac attatctctg
     8401 tacttaaatt aaattcccac aatagataac agga
//
LOCUS       DAPHCV010000162         8307 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.164, whole genome shotgun sequence.
ACCESSION   DAPHCV010000162 DAPHCV010000000
VERSION     DAPHCV010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.164"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            95..1375
                     /gene="wcaK"
                     /locus_tag="RH033_004247"
     CDS             95..1375
                     /gene="wcaK"
                     /locus_tag="RH033_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HDV2993343.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAVQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFSTPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLNEAVSRERQTGMQMVKSVLERIGEAK"
     gene            1372..2592
                     /gene="wcaL"
                     /locus_tag="RH033_004248"
     CDS             1372..2592
                     /gene="wcaL"
                     /locus_tag="RH033_004248"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HDV2993344.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLAARTRWLQDEPTGKGAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFYADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDSDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            2603..3997
                     /gene="wcaM"
                     /locus_tag="RH033_004249"
     CDS             2603..3997
                     /gene="wcaM"
                     /locus_tag="RH033_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HDV2993345.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPAQQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGLICDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGKEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKATLLSLANVHAVNEKGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            4172..5065
                     /gene="galF"
                     /locus_tag="RH033_004250"
     CDS             4172..5065
                     /gene="galF"
                     /locus_tag="RH033_004250"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HDV2993346.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLELRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGCS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIIEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGYSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            5438..6523
                     /gene="rfbB"
                     /locus_tag="RH033_004251"
     CDS             5438..6523
                     /gene="rfbB"
                     /locus_tag="RH033_004251"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDV2993347.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFVHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNKEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KKLPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            6523..7422
                     /gene="rfbD"
                     /locus_tag="RH033_004252"
     CDS             6523..7422
                     /gene="rfbD"
                     /locus_tag="RH033_004252"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDV2993348.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPD
                     GVAETIRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGSGDRPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPDVAGLYHLVAS
                     GTTTWYDYAALVFEEARKADIPLALNKLNAVPTTAYPTPACRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            7480..>8307
                     /gene="rfbA"
                     /locus_tag="RH033_004253"
     CDS             7480..>8307
                     /gene="rfbA"
                     /locus_tag="RH033_004253"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDV2993349.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPTPDGLAQAFIIGEEFIGG
                     DDCALILGDNIFYGHDLPKLMDAAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIETRQGLKVSCPEEIAYRKGFISDEQMQIIASKL
                     "
BASE COUNT         2240 a         1919 c         2095 g         2053 t
ORIGIN      
        1 ggcctgataa gacgcgtcag cgtcgcatca ggcaaaaaac ggcaacgaaa acaacaaaca
       61 cagcctcacc tggcatcaaa tttatgagga aaaaatgaaa ttacttattc tgggcaacca
      121 cacttgcggc aaccgcggcg acagcgccat cttgcgcggc ttacttgatg ccatcaacat
      181 cctcaatcca cacgctgaag tggacgtgat gagccgctat ccggtcagtt cttcctggct
      241 gctcaaccgc ccggtaatgg gcgatccgct gttcctgcaa atgaaacaac acaacagcgc
      301 ggcgggcgtt gtcgggcgcg taaaaaaagt cctccgtcgc cgctaccagc accaggtatt
      361 gctctcacgc gttaccgaca ctggcaagct gcgtaatatc gccattgccc agggtttcac
      421 tgactttgtg cgcctgctgt caggttacga cgccattatt caggtcggcg gatcgttttt
      481 tgtcgatctc tacggcgtgc cgcagtttga acacgcgctt tgcacgttta tggcgaaaaa
      541 gccgttgttt atgattggtc acagcgtcgg cccgttccag gatgagcaat ttaaccaact
      601 ggcgaactac gtattcggtc actgcgacgc gctgatcctg cgcgaatcgg tcagcctgga
      661 tctaatgaaa cgcagcaata tcactaccgc aaaagttgaa catggcgtcg ataccgcgtg
      721 gttggtcgat caccacacag aagacttcac cgccagctat gccgttcaac actggctgga
      781 cgttgccgta caacagaaaa cggtagcaat taccctgcgc gaactggcac cgttcgacaa
      841 acgtctcggc accactcagc aagcgtatga aaaagccttt gccggggtgg tcaatcgcat
      901 tctcgacgaa gggtatcagg tgattgcgct ttccacctgt acgggcatcg acagctataa
      961 caaagacgac cgcatggtgg cgctcaacct gcgccagcac atcagcgatc ctgcccgtta
     1021 tcacgtagtg atggatgaac tcaacgatct ggaaatgggc aaaattctcg gtgcctgtga
     1081 actcaccgtc ggtacgcgcc tgcactcagc cattatctcg atgaatttct ccactccggc
     1141 aattgccatc aactacgaac ataaatccgc cgggatcatg caacaactgg ggctaccaga
     1201 gatggcaatt gatatccgtc atttattaga cggcagcctg caagcgatgg ttgcggatac
     1261 cttaggtcag cttccggcgc tgaacgcacg acttaacgaa gctgtcagtc gcgaacgtca
     1321 gacgggaatg cagatggtga aatctgtgct tgaacgcatc ggggaggcaa aatgaaggtc
     1381 ggcttctttt tactgaaatt tccgctgtcg tcggaaacct tcgttctcaa ccaaatcact
     1441 gcgtttattg atatgggctt tgaggtggag attgtcgcgt tgcaaaaagg cgatacagag
     1501 aacacccacg cggcatggac gaaatacaac cttgccgcca gaacccgctg gttacaggac
     1561 gaacctacgg gcaaaggggc gaagctgcgc caccgcgcca gccagacctt acgcggcatt
     1621 catcgtaaaa atacctggca ggcgcttaac ctcaaacgct atggtgccga gtcgcggaac
     1681 ctgattttgt ctgccatttg cggccaggtc gcaacaccat tttatgccga tgtctttatc
     1741 gctcattttg gccctgcggg ggtaaccgca gcaaaactac gcgaactggg tgtcattcgc
     1801 ggcaaaatag ccaccatttt ccacggcatt gatatttcca gtcgggaagt gctcaaccac
     1861 tacactcctg aatatcagca actgttccgc cgtggcgacc tgatgctacc gatcagcgat
     1921 ctgtgggccg gaaggctgca aaaaatgggc tgcccgaggg aaaaaattgc cgtatcgcgc
     1981 atgggcgtgg acatgacgcg ctttagcccg cgtccggtga aagcgcccgc aacgccgctg
     2041 gaaatcattt ccgtcgcacg tttaaccgag aaaaaaggcc tgcatgtggc gattgaagcc
     2101 tgccgtcagt tgaaagagca gggcgtggca tttcgctatc gcattctcgg cattggcccg
     2161 tgggaacgac gcctgcgcac gctcatcgaa caatatcaac tggaagatgt ggtagagatg
     2221 ccgggcttta aaccgagtca tgaagtgaaa gagatgctcg atgacgcgga tgtcttcctg
     2281 ttgccatcgg ttacgggtgc ggatggcgat atggaaggca tcccggtggc gctgatggaa
     2341 gcgatggcgg tcggcattcc ggttgtttct actctgcata gcggaatacc ggaactggtg
     2401 gaggccgata aatccggctg gctggtgcct gagaacgatg ctcgcgcact ggcgcaacga
     2461 ctggcggcgt ttagccaact ggacagcgac gaactggctc ctgtcgtcaa acgtgctcgc
     2521 gaaaaagtcg aacacgattt taaccagcag gtgatcaatc gagaactcgc cagcttgctg
     2581 caagctttat agaggagaac gcatgccatt taaaaaactc tcccgacgca ccttcctgac
     2641 ggcaagctcg gcgcttgcct tcctccatac gcctttcgcc cgcgcacttc ccgctcaaca
     2701 aagcgttaac attaacgact acaaccctca cgactggatc gcctcattta aacaagcctt
     2761 cagcgaaggg caaacggtcg tcgtgcccgc cggattgatt tgtgacaata tcaacaccgg
     2821 catcttcatt cctcctggaa aaacgttaca catccttgga agcctgcgcg gcaacggcag
     2881 agggcgtttt gtcttacagg atggcagcca ggtgacaggg aaggagggcg gcagcatgca
     2941 taatatcacc ctggatgtgc gcggttctga ctgcaccatt aaagggctgg caatgagcgg
     3001 ctttggcccg gtaacgcaga tttatatcgg cggcaaaaac aaacgggtca tgcgcaacct
     3061 gaccatcgat aacctcactg tcagccacgc taattacgcc atcttacgcc agggatttca
     3121 taaccagatt atcggtgcca acatcaccaa ctgtaagttc agcgacttac agggcgacgc
     3181 cattgaatgg aacgtggcaa ttaacgacag tgatattttg atatctgacc atgtcatcga
     3241 gcgcatcaac tgtaccaacg gcaaaatcaa ctggggcatt ggcataggcc ttgcggggag
     3301 tacttatgat aataactacc cggaagatca ggcagtgaaa aactttgtcg tggcgaatat
     3361 cacgggatcg gattgtcggc agttgatcca tgttgaaaat ggtaaacatt ttgttattcg
     3421 taatatcaaa gcccgcaata tcacaccgga cttcagtaag aaagcaggta ttgataacgc
     3481 cacggttgct atttacggtt gtgacaattt cgtgattgat aatattgaaa tgattaatag
     3541 tgccgggatg ttaatcggct atggggtaat taaaggcaaa tatctctcga taccgcaaaa
     3601 tttccgagtg aataatattc aactggataa cacccatctt gcttacaaat tgcgcggcat
     3661 ccaaatctct gccgggaatg ctgtctcctt tgtggcgctg actaacattg agatgaagcg
     3721 tgcgtcgctg gagttacaca acaaaccgca acaccttttt atgcgaaata tcaaggtgat
     3781 gcaggaatcc tcagttggac cagcattgag tatgaacttt gacatgcgca aagacgttcg
     3841 aggcgtgttt atggcgaaaa aagcaacact gctgtctctt gcaaatgttc atgcggtgaa
     3901 tgaaaaaggg caaagctccg tcgatattga caggattaat caccatattg ttaatgtgga
     3961 aaagattaac tttagattgc cggaacggag ggagtagatt tgcgaccatt cctggaaaaa
     4021 tggagtcata cttaggaaca atgctactgc aatccacaac gaagcggcgt aacatcacaa
     4081 gtaattcagt aatcaattca gggtaattga tgctggcgaa aaaaatcgaa caagctataa
     4141 ttcagcaacc attttacagg tggatgaaat aatgacgaat ttaaaagcag ttattcctgt
     4201 agcgggtcta gggatgcata tgttgcctgc cactaaggcg attcccaaag agatgctacc
     4261 gatcgtcgac aagccaatga ttcagtacat tgttgacgag attgtggctg cagggatcaa
     4321 agaaatcctc ctggtaactc acgcgtccaa gaacgcggtc gaaaaccact tcgacacctc
     4381 ttatgaatta gaatctctcc ttgaactgcg cgtgaagcgt caactgctgg cggaagtgca
     4441 atctatctgc ccgccgggcg tgaccattat gaacgtgcgt cagggcgaac ctttaggttt
     4501 aggccactcc attttgtgtg cacgccccgc cattggtgac aacccgtttg tcgtggtact
     4561 gccagacgtt gtgatcgacg atgccagcgc cgacccgcta cgttacaacc ttgctgccat
     4621 gattgcgcgc ttcaacgaaa cgggctgcag ccaggtgctg gcaaaacgta tgccgggtga
     4681 cctctctgaa tactccgtca ttcagaccaa agaaccactg gatcgtgaag gtaaagtcag
     4741 ccgcattatt gaatttatcg aaaaaccgga tcagccgcaa acgctggact cagacatcat
     4801 ggccgttggt cgctatgtgc tttctgccga tatttggccg gaacttgaac gcacgcagcc
     4861 tggtgcatgg ggacgtattc agttgactga tgccattgcc gaactggcga aaaaacagtc
     4921 cgttgatgcc atgctgatga ccggctacag ctacgactgc ggtaaaaaaa tgggttatat
     4981 gcaggcgttt gtgaagtatg gactgcgtaa cctaaaagaa ggggcgaagt tccgtaaagg
     5041 gattgagaag ctgttaagcg aataatgaaa atctgaccgg atgtaacggt tgataagaaa
     5101 atcataacgg cagtgaagat tcgcggggaa agtaatttgt tgcgaatttt cctgccgttg
     5161 ttttatataa acaatcagaa taacagagag ttagcaatag gattttcgtc aaagttttcc
     5221 aggattttcc ttgtttccag agcggattgg taagacaatt agcgtatgaa tttttcgggt
     5281 ttggcgcgag tgggtaacgc tcgccacatc gtaggcatgc atgcagtgct ctggtagctg
     5341 taaagccagg ggcggtagcg tgtgttgata cctctattaa ttaaactgag ggccgcttat
     5401 ttcacagcat gctctgaagt aatatggaat aaattaagtg aaaatacttg ttactggtgg
     5461 tgcaggattt attggttctg cggtagttcg tcacattata aataatacgc aggatagtgt
     5521 tgttaatgtc gataaattaa cgtacgccgg aaacctggaa tcacttgctg atgtttctga
     5581 ttctgaacgc tatgtttttg tacatgcgga tatttgcgat gctgctgcaa tggcgcggat
     5641 ttttgctcag catcagccgg atgcagtgat gcacctggct gctgaaagcc atgtggatcg
     5701 ttccatcact ggccctgcgg catttattga aaccaatatt gttggtacat atgtcctttt
     5761 ggaagccgct cgcaattatt ggtctgctct tgatagcgac aagaaaaaca gcttccgttt
     5821 tcatcatatt tctactgacg aagtctatgg tgatttgcct catcctgacg aggtaaataa
     5881 taaagaagaa ttacccttat ttactgagac gacagcctac gcgccaagca gcccttattc
     5941 cgcatccaaa gcatccagcg atcatttagt ccgcgcgtgg aaacgtacct atggtttacc
     6001 gaccattgtg actaattgct ctaacaatta tggtccttat catttcccgg aaaaattaat
     6061 tccattggtt attctcaatg ctctggaagg taaaaaatta cctatttatg gtaaagggga
     6121 tcaaattcgc gactggttgt atgttgaaga tcatgcgcgt gcgttatata ccgtcgtaac
     6181 cgaaggtaaa gcgggtgaaa cttataacat tggtggacac aacgaaaaga aaaacataga
     6241 tgtagtgctc actatttgtg atttgctgga tgagattgta ccgaaagaga aatcttatcg
     6301 tgagcaaatc acttatgttg ccgatcgtcc gggacacgat cgccgttatg ccattgatgc
     6361 tgagaagatt ggtcgcgaat tgggatggaa accacaggaa acgtttgaga gcgggattcg
     6421 gaagacagtg gaatggtacc tgtccaatac aaaatgggtt gataatgtga aaagtggtgc
     6481 ctatcaatcg tggattgaac agaactatga gggccgccag taatgaatat cctccttttt
     6541 ggcaaaacag ggcaggtagg ttgggaacta cagcgtgctc tggcaccttt gggtaatttg
     6601 attgctcttg atgttcactc tactgattat tgcggtgatt ttagtaatcc tgacggtgta
     6661 gctgaaacca taagaagcat tcggcctgat attattgtca acgcagccgc tcacaccgca
     6721 gtagacaaag cagaatcaga accggagttt gcacaattac ttaacgcaac aagtgtcgaa
     6781 gcgattgcga aagcagcaaa tgaagttgga gcttgggtta tacactactc caccgattat
     6841 gttttccctg gcagtggtga caggccatgg ctggaaacgg atgcaacagc gccgctaaat
     6901 gtttacggtg aaactaagct agctggggaa aaagcgttac aagaacattg cgcaaagcat
     6961 cttattttcc gtaccagctg ggtatacgct ggtaaaggaa ataactttgc caaaacgatg
     7021 ttgcgtctgg caaaagaacg cgaagagttg gctgtgataa acgatcaatt tggcgcacca
     7081 acgggtgctg agctgctggc cgattgtaca gcacatgcca ttcgtgtcgc actgaataaa
     7141 ccggatgtcg caggcttgta ccatctggta gccagtggta ccacgacctg gtacgattat
     7201 gctgcgctgg tttttgaaga ggcgcgcaaa gcagatattc ccctcgcact caacaaactc
     7261 aatgcggtac caacgacagc ctatcctaca ccagcttgtc gtccacataa ctctcgactt
     7321 aatacagaaa aatttcagca gaattttgcg cttgtcttgc ctgactggca ggttggtgtg
     7381 aaacgcatgc tcaacgaatt atttacgact acagcaattt aatagttttt acatcttgtt
     7441 cgtgatgatg gagcaagatg aattaaaagg aatgatgaaa tgaaaacgcg taagggtatt
     7501 attttagcgg gtggttctgg tactcgtctt tatcctgtga ctatggcagt cagtaaacag
     7561 ctgctaccga tttatgataa accgatgatc tattacccgc tctctacact gatgttggcg
     7621 ggtattcgcg atattttgat tattagtacg ccacaggata ctcctcgttt tcaacaactg
     7681 ctgggtgacg gtagccagtg ggggctaaat cttcagtaca aagtgcaacc gactccagat
     7741 gggcttgcgc aggcatttat tatcggtgaa gagtttattg gtggtgatga ttgtgctttg
     7801 attcttggtg ataatatctt ttacggtcac gatctgccga agttaatgga tgccgctgtt
     7861 aacaaagaaa gtggtgcaac ggtatttgcc tatcacgtaa atgatcctga acgctatggt
     7921 gtcgttgagt ttgataaaaa cggtactgca atcagcctgg aagaaaaacc gttacaacca
     7981 aaaagtaatt atgcggtaac cgggctttat ttctatgata acgatgttgt cgaaatggcg
     8041 aaaaatctta agccttctgc ccgcggtgaa ctggaaatta ccgatattaa ccgtatatat
     8101 atggaacaag ggcgtttatc tgttgccatg atgggccgtg gttatgcgtg gttagacacg
     8161 gggacacatc agagcctgat tgaggcaagc aacttcattg ccacaattga aacacgtcag
     8221 gggctaaaag tttcttgtcc agaagagatt gcgtatcgga aagggtttat tagtgatgag
     8281 caaatgcaga taatagcctc taaatta
//
LOCUS       DAPHCV010000163         8066 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.165, whole genome shotgun sequence.
ACCESSION   DAPHCV010000163 DAPHCV010000000
VERSION     DAPHCV010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.165"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            2..1723
                     /gene="iucA"
                     /locus_tag="RH033_004254"
     CDS             2..1723
                     /gene="iucA"
                     /locus_tag="RH033_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HDV2993350.1"
                     /translation="MLPSEKSATDVAAQCFLNALIRETKDWQLAEYPPDELIIPLDEQ
                     KSLHFRVAYFSPTQHHRFAFPAHLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLCE
                     TFHQRVLESYAHTQQTIDARHDWAILREKALNFGEAEQALLTGHAFHPAPKSHEPFNR
                     QEAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDNQ
                     WLFPLHPWQGEYLLQQVWCQALFAKGLIRDLGEAGTSWLPTTSSRSLYCATSRDMIKF
                     SLSVRLTNSVRTLSVKEVERGMRLARLAQTDGWQMLQARFPTFRVMQEDGWAGLRDLN
                     GNIMQESLFSLRENLLLEQPQSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITVQ
                     QAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSAF
                     MPHATEWLDTIDEAQAENIFTREQLLRYFPYYLLVNSTFAVTAALGAAGLDSEANLMA
                     RVRTLLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFAN
                     PLQAQEV"
     gene            1724..2671
                     /gene="iucB"
                     /locus_tag="RH033_004255"
     CDS             1724..2671
                     /gene="iucB"
                     /locus_tag="RH033_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HDV2993351.1"
                     /translation="MSGANIVHSGYGLRCEKLDKPLNLGWGLDNSAVLHWPGELPTGW
                     LCDALDQIFIAAPQLSAVVLPWSEWCEEPQALTLFGQVQSDIIHRSAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQIAYLERQLTSKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGVTHYLLLNEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            2671..4413
                     /gene="iucC"
                     /locus_tag="RH033_004256"
     CDS             2671..4413
                     /gene="iucC"
                     /locus_tag="RH033_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HDV2993352.1"
                     /translation="MNHKDWDLVNRRLVAKMLSELEYEQVFHAESQGDDRYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCADEPVLAQTLLMQLKQVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLNADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     ANTFRLHWLAVKREHMIWRCDNEMDIHQLLTAAMDPQEFARFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEEFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMKKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLED
                     LQNPLWLVTQEYES"
     gene            4410..5687
                     /gene="iucD"
                     /locus_tag="RH033_004257"
     CDS             4410..5687
                     /gene="iucD"
                     /locus_tag="RH033_004257"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HDV2993353.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELDCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKRRLFLVQTSQGEYFARNICLGTGKQPYLPPCVK
                     HMTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLEEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWKLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFVVNASMQTHGIAEPQLSL
                     MAWRSARILNRVMGRDLFDLSMPPALIQWRSGT"
     gene            5769..7970
                     /gene="iutA"
                     /locus_tag="RH033_004258"
     CDS             5769..7970
                     /gene="iutA"
                     /locus_tag="RH033_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HDV2993354.1"
                     /translation="MMISKKYTLWALNPLLLTMMAPAVAQQTDDETFVVSANRSNRTV
                     AEMAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSRSRTNYGMNVRGRPLVVL
                     VDGVRLNSSRTDSRQLDSIDPFNIDHIEVISGATSLYGGGSTGGLINIVTKKGQPETM
                     MEFEAGTKSGFSSSKDHDERIAGAVSGGNEHISGRLSVAYQKFGGWFDGNGDATLLDN
                     TQTGLQYSDRLDIMGTGTLNIDESRQLQLITQYYKSQGDDDYGLNLGKGFSAIRGTST
                     PFVSNGLNSDRIPGTERHLISLQYSDSAFLGQELVGQVYYRDESLRFYPFPTVNANKQ
                     VTAFSSSQQDTDQYGMKLTLNSKPMDGWQITWGLDADHERFTSNQMFFDLAQASASGG
                     LNNKKIYTTGRYPSYDITNLAAFLQSGYDINNLFTLNGGVRYQYTENKIDDFIGYAQQ
                     RQIAAGKATSADAIPGGSVDYDNFLFNAGLLMHITERQQAWLNFSQGVELPDPGKYYG
                     RGIYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGNNLRTQIAAYYSISDKS
                     VVANKDLTISVVDDKRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGTWQKYDV
                     KTASPSKATAYIGWAPDPWSLRVQSTTSFDVSDAQGYKVDGYTTVDLLGSYQLPVGTL
                     SFSIENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         1869 a         2136 c         2162 g         1899 t
ORIGIN      
        1 gatcctgccc tctgaaaaat ccgccacaga tgtggctgcg cagtgcttcc tgaatgcctt
       61 gattcgtgaa accaaagact ggcaactggc ggaatacccg ccagacgaat tgattatccc
      121 gctggacgaa cagaagtcgc tccatttcag agtggcttat ttctccccaa cccagcatca
      181 ccgctttgca tttcctgctc atctggtcac ggcatcaggc agttatcctg tcgactttac
      241 cactctctcc cggcttatta ttgataagct ccggcatcaa ctttttctgc cagttcccct
      301 ctgcgaaact ttccaccaac gcgtgctgga aagctacgcc catacgcaac agacaattga
      361 tgcccgccat gactgggcca tcctgcgtga aaaagcgttg aattttggcg aggctgagca
      421 ggcactgctg acaggacacg ctttccaccc tgcacctaag tctcatgaac cgtttaaccg
      481 ccaggaagct gaacgatacc tgcctgacat ggcacctcac ttcccgctgc ggtggttttc
      541 ggtggataaa acgcaaatcg ctggtgaaag tctgcatctt aaccttcaac agcggttgac
      601 gcgatttgcc gcagaaaatg cgccccagtt actcaacgaa ttaagtgaca atcaatggct
      661 gttcccgctg cacccgtggc agggagaata tcttttgcag caagtgtggt gccaggcact
      721 ttttgctaaa ggacttatca gagacttagg cgaggccggc acgtcgtggc tgccgaccac
      781 ctcttcccgc tccctctact gtgctaccag ccgcgatatg atcaagttct ccctgagcgt
      841 tcggctgacc aactccgtcc gtactctgtc tgtgaaagag gtggagcgag gaatgcgcct
      901 ggcacgtctg gcgcaaaccg acggctggca gatgctacag gcccgcttcc ctactttccg
      961 ggtaatgcag gaggacggct gggccgggct gcgcgatctt aacggcaaca tcatgcagga
     1021 aagtttgttt tccctgcgtg aaaacctgct gctggagcag ccgcaaagcc agaccaacgt
     1081 tctggtttcc ctgactcagg ccgcaccgga tggcggtgat tcgctgctgg tttcggcggt
     1141 aaaacgcctg agcgatcgcc tcggtatcac tgtgcaacag gccgcccacg catgggtcga
     1201 tgcttactgt cagcaggtgc tgaagcctct gtttacggct gaagcggatt acggcctggt
     1261 gctgctggcg catcagcaaa atattcttgt gcagatgctt ggggatctgc cggtcggatt
     1321 tatttaccgt gactgccagg gcagcgcgtt tatgcctcac gcgacagagt ggctcgatac
     1381 cattgacgag gcgcaggcgg aaaatatttt cacccgtgag cagttgctgc gctatttccc
     1441 ttattacctg ctggttaact ccacttttgc cgtaactgct gcgctgggtg ctgccgggct
     1501 ggacagcgaa gcgaatctga tggctcgtgt acgaacattg ctggctgaag tgcgtgacca
     1561 ggtgactcat aaaacctgtc tcaactatgt tctggaaagt ccgtactgga acgtaaaagg
     1621 taactttttc tgttatctga acgatcataa cgagaacacc atcgttgacc cttcggtgat
     1681 ctattttgat ttcgctaacc cgctgcaggc tcaggaggtc tgaatgtctg gggcaaacat
     1741 tgttcacagc ggatatggac tgcgctgtga aaaactcgac aagcctctga atcttggctg
     1801 ggggctggac aatagcgcgg tgttgcactg gcccggggag ctgccaacag ggtggctgtg
     1861 cgacgcgctg gatcagatat ttatcgccgc accacaactt tcagcagtgg ttcttccctg
     1921 gtccgaatgg tgtgaggagc cacaggcgct gacgcttttc ggacaggtac aaagcgacat
     1981 tatccatcgt tccgctttct ggcagttacc gttatggctg agttctccgg caaaccgggc
     2041 ctccggtgaa atggtttttg atgcagagcg tgagatttat ttcccgcagc gccccccccg
     2101 tccgcagggt gaagtttatc gtcgttacga tccacgcatt cgcaggatgc tgagtttccg
     2161 cattgccgat cccgtttctg atgcagaacg tttcactcgc tggatgaacg atccgcgcgt
     2221 tgagtatttc tgggagcaaa gtggctcact ggaggtacag atcgcctatc tggaacgcca
     2281 gctcaccagt aaacatgcat tcccgctgat tggttgtttc gacgatcggc cgttcagtta
     2341 tttcgaaatc tactgggcgg cggaagaccg cattggtcgc cactattcgt ggcagccctt
     2401 tgaccgtggc ctgcatctgc tggttggtga acagcaatgg cgcggagccc actatgtgca
     2461 aagctggctg cgcggggtga cacattacct gctgctgaat gagccacgta cgcagcgcac
     2521 agtactggag ccacgcaccg ataaccagcg cctgttccgc catcttgagc ctgcgggata
     2581 ccggacaatt aaagagttcg acttcccaca taaacgctcg cgcatggtga tggcggatcg
     2641 tcatcacttc ttcacggagg tcggtctgta atgaatcaca aagactggga tttggtcaac
     2701 cgccgcctgg tggcaaaaat gttgtctgag ctggagtatg agcaggtttt ccacgcggaa
     2761 tctcagggcg atgaccgcta ctgcattaac ctgccgggag cacaatggcg cttcatcgct
     2821 gaacgtggta tctggggctg gctctggatt gatgctcaaa ctctgcgctg cgcggacgag
     2881 ccagtactgg ctcagacgct gctgatgcag ctaaagcagg tactgtcaat gagcgatgca
     2941 accgttgctg agcatatgca ggatttgtat gccacgctgc tgggcgacct gcaactactg
     3001 aaagcccgtc gcgggctgag cgccagtgac ctgattaatc ttaatgccga ccgcctgcaa
     3061 tgcctgctga gcggtcatcc taaattcgtt tttaataaag gtcgccgtgg ctggggtaaa
     3121 gaggcgctgg aacgatatgc gccagagtat gccaacacct tcagactgca ctggctggcg
     3181 gtaaaacgtg aacatatgat ctggcgctgt gataacgaga tggatattca tcagttgttg
     3241 acggccgcaa tggatccgca ggagtttgcc cgcttcagtc aggtctggca ggaaaacgga
     3301 ctggatcata actggctgcc gctgccggta catccgtggc agtggcagca aaaaatcgct
     3361 accgacttca tcgctgattt tgccgaaggc aggatggtgt ctctcggcga gtttggcgac
     3421 cagtggctcg cccagcagtc gctgcgtacc ctgaccaacg ccagtcgccg gggcgggctg
     3481 gatatcaagc tgccgctgac catctacaac acctcatgct accgggggat ccctggcaga
     3541 tacatcgctg ccggaccact ggcttcacgc tggctacaac aggtttttgc gaccgacgcc
     3601 accctagtgc aaagcggcgc agtgatactt ggtgaaccgg ctgcaggcta tgtctcccat
     3661 gaaggctatg ccgcgcttgc ccgggctccc tatcgctacc aggaaatgct tggtgttatc
     3721 tggcgggaga atccgtgccg ctggctgaaa ccggatgaaa gtccggttct gatggcaaca
     3781 ctgatggagt gcgacgaaaa caatcagccg ctggcaggcg catatataga ccgctccggg
     3841 ctggacgctg aaacctggct tacgcaactg ttccgggtgg tcgtggttcc tctgtatcac
     3901 ctgctttgcc gctacggtgt cgcgcttatt gcacatggac aaaatataac tctcgccatg
     3961 aaagaggggg ttccacagcg tgttctgctg aaagacttcc agggcgatat gcgactggtg
     4021 aaagaagagt tccccgaaat ggactctttg cctcaggagg ttcgtgatgt tacatcccgc
     4081 ctgagtgcgg actacttaat ccatgatttg cagacgggtc acttcgtgac agtactgcgt
     4141 tttatttcgc cactgatggt tcgtcttggc gtacctgaaa ggcgatttta tcaactgctg
     4201 gcagcagtgt tgagtgatta catgaaaaaa catccacaaa tgtcagagcg ttttgcgctt
     4261 ttctcacttt tcaggccaca aatcattcgc gtggtgctga acccggtaaa actgacctgg
     4321 cctgatctgg atggcggcag ccgcatgctg ccgaattacc ttgaggatct gcaaaatccg
     4381 ctgtggctgg taactcagga atatgaatca tgaaaaaaag tgtcgatttt attggtgtag
     4441 ggacagggcc atttaatctc agcattgctg cgttgtcaca tcagatcgaa gaactggact
     4501 gtctcttctt tgatgaacat cctcattttt cctggcatcc gggtatgctg gtaccggatt
     4561 gtcatatgca gaccgtcttt ctgaaagatc tggtcagtgc tgttgcacct acaaatccct
     4621 acagttttgt taactatctg gtgaagcaca aaaagttcta tcgcttcctt acaagcagac
     4681 tacgtacagt atcccgtgaa gagttttctg actacctccg ctgggctgct gaagatatga
     4741 ataacctgta tttcagtcat accgttgaaa acattgattt cgataaaaaa cgtcgattgt
     4801 ttctggtgca aaccagccag ggagaatatt ttgcccgcaa tatctgcctt ggtacaggaa
     4861 aacaacctta tttaccaccc tgtgtgaagc atatgacaca atcctgtttc catgccagtg
     4921 aaatgaatct tcgtcggccg gaccttagtg gaaaacggat aaccgtggtt ggtggaggac
     4981 agagtggtgc agacctgttc cttaatgcat tacgcgggga atggggagaa gcggcggaaa
     5041 taaactgggt ctcccggcgt aataatttta acgcactgga tgaggctgct tttgctgatg
     5101 agtattttac acctgaatat atttcaggct tctccggact ggaggaagat attcgccatc
     5161 agttactgga tgagcagaaa atgacatcgg atggcatcac tgccgattct ttactgacca
     5221 tttatcgtga gttgtaccac cgttttgaag ttctgagaaa accaagaaat atccgtctgc
     5281 tacccagccg ctcggtaaca actctggaaa gtagtggtcc tggctggaag ttgctgatgg
     5341 agcatcatct ggatcagggc agggagagcc tggaaagtga tgtggtgatt ttcgccacag
     5401 gttaccgttc tgcgttgcca caaatacttc cctcactgat gcccctgatc accatgcacg
     5461 ataagaacac ctttaaagtg cgtgatgact tcactctgga atggagtggc ccgaaagaga
     5521 acaacatctt cgtggtcaac gccagtatgc aaacccatgg catcgccgaa ccccagctca
     5581 gcctgatggc atggagatct gcacgtattc ttaatcgcgt aatgggacgt gatttattcg
     5641 atctcagtat gccgcccgcc ctgattcagt ggcgcagcgg cacctaggga aaacgcagcc
     5701 ggaggctgct tctttaactc gctacacagc atctttgggc tgattttttc cgcccgtatg
     5761 gaggaataat gatgataagc aaaaagtata cgctttgggc tctcaaccca ctgcttctta
     5821 ccatgatggc gccagcagtc gctcaacaaa ccgatgatga aacgttcgtg gtgtctgcca
     5881 accgcagcaa tcgcaccgta gcggagatgg cgcaaaccac ctgggttatc gaaaacgccg
     5941 aactggaaca gcagattcag ggcggcaaag agcttaaaga cgcactggct cagctgatcc
     6001 ctggccttga cgtcagcagc cggagccgca ccaactacgg tatgaatgtg cgtggccgcc
     6061 cgctggtcgt gctggttgac ggcgtgcgtc tcaactcttc acgtaccgac agccgacaac
     6121 tggactctat agatcctttt aatatcgacc atattgaagt gatctccggt gcgacgtccc
     6181 tgtacggcgg cggcagtacc ggtggcctga tcaacatcgt gaccaaaaaa ggccagccgg
     6241 aaaccatgat ggagtttgag gctggcacca aaagtggctt tagcagcagt aaagatcacg
     6301 atgaacgcat tgccggagct gtctccggcg gaaatgagca tatctccgga cgtctttccg
     6361 tggcatatca gaaatttggc ggctggtttg acggtaacgg cgatgccacc ttgcttgata
     6421 acacccagac cggcctgcag tactccgatc ggctggacat catgggaact ggtacgctga
     6481 acatcgatga atcccggcag cttcagttga tcacacagta ctataaaagc cagggcgacg
     6541 acgattacgg gcttaatctc gggaaaggct tctctgccat cagagggacc agcacgccat
     6601 tcgtcagtaa cgggctgaat tccgaccgta ttcccggcac tgagcggcat ttgatcagcc
     6661 tgcagtactc tgacagcgct tttctgggac aggagctggt cggtcaggtt tactaccgcg
     6721 atgagtcgtt gcgattctac ccgttcccga cggtaaatgc gaacaaacag gtgacggctt
     6781 tctcttcgtc acagcaggac accgaccagt acggcatgaa actgactctg aacagcaaac
     6841 cgatggacgg ctggcaaatc acctgggggc tggatgctga tcatgagcgc tttacctcca
     6901 accagatgtt cttcgacctg gctcaggcaa gcgcttccgg agggctgaac aacaagaaga
     6961 tttacaccac cgggcgctat ccgtcgtatg acatcaccaa cctggcggcc ttcctgcaat
     7021 caggctatga catcaataat ctctttaccc tcaacggtgg cgtacgctat cagtacactg
     7081 aaaacaagat tgatgatttc atcggctacg cgcagcaacg gcagattgcc gccgggaagg
     7141 ctacatccgc cgacgccatt cctggcggct cagtcgatta cgacaacttc ctgttcaacg
     7201 ccggtctgct gatgcacatc accgaacgcc agcaggcatg gctcaacttc tcccagggcg
     7261 tggagctgcc ggacccgggt aaatactatg gtcgcggcat ctatggtgct gcagtgaacg
     7321 gccatcttcc tctaacaaag agtgtgaacg tcagcgacag caagctggaa ggcgtgaaag
     7381 tcgattctta tgagctgggc tggcgcttta ctggcaataa tctgcgtacc caaatcgcgg
     7441 cctactattc gatttctgat aagagcgtgg tggcgaataa agatctgacc atcagcgtgg
     7501 tggacgacaa acgccgtatt tacggcgtgg aaggtgcggt ggactacctg attcctgata
     7561 ctgactggag taccggagtg aacttcaacg tgctgaaaac tgagtcgaaa gtgaacggta
     7621 cctggcagaa atacgatgtg aagacagcaa gcccatcaaa agcgacagcc tacattggct
     7681 gggcaccgga cccgtggagt ctgcgcgtgc agagcaccac ctcctttgac gtgagcgacg
     7741 cgcagggcta caaggtcgat ggctatacca ccgtggatct gctcggcagt tatcagcttc
     7801 cggtgggtac actcagcttc agcattgaaa acctcttcga ccgtgactac accactgtct
     7861 gggggcagcg tgcaccactg tactacagcc cgggttacgg cccagcgtca ctgtacgact
     7921 acaaaggcag gggccgaacc tttggtctga actactctgt gctgttctga ccggtattcc
     7981 tttacaacaa aggtacgctg atatcaacat ggccgctgac agccaagttg atatcatata
     8041 atacacgaca taatctgtag tcaggg
//
LOCUS       DAPHCV010000164         7987 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.166, whole genome shotgun sequence.
ACCESSION   DAPHCV010000164 DAPHCV010000000
VERSION     DAPHCV010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.166"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1092
                     /locus_tag="RH033_004259"
     CDS             <1..1092
                     /locus_tag="RH033_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDV2993355.1"
                     /translation="EVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHI
                     ARRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYE
                     IASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRID
                     GYKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRG
                     SKMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYD
                     TTQDVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLM
                     LQWWADFLDANREQCISPFEYAKINNPLK"
     gene            complement(1454..1618)
                     /locus_tag="RH033_004260"
     CDS             complement(1454..1618)
                     /locus_tag="RH033_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV2993356.1"
                     /translation="MSNITRNSLVVTGVLAALMAGNVALASDVPTEVTLIDKQTLVRN
                     NGAEVQSADK"
     gene            1955..3511
                     /locus_tag="RH033_004261"
     CDS             1955..3511
                     /locus_tag="RH033_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012884439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDV2993357.1"
                     /translation="MHDYLLFLLGLTPLLAMIILILKVKMPIHYAVLSTLLITLILGG
                     VFWQTPVMDMGSAVVYGAIKGLWPIVIVILAAIYSYNLMNATKSMDVLRNVLASVSDD
                     KRIQVLLISWCFGGFLEAAAGYGTAVAIPIGILIALGFNPLKAAIASLVANTVPTAFG
                     AVGIPVSVLAEQVNLPVTTLSGTIILQLALFNILLPFVIVAIIGGGMKAIRGVGLITL
                     MCGISTLIPQYLVAVNLGAELPAFAGSLVSLIVIVFMAKNRKEKTEPEFLIEASADSA
                     SALSYSRGELFRACSIYILIFLFILLCSPLFPTIKSAVSQIASSLSFSLSNGSNLSMK
                     IEWVATPGVLIIIASVIGGLIQKASLSCLLNVFVSTVKQLKNSIIAISAIVAMATVMD
                     VCGLIGTLAQSLVDLTGGAYLFIAPVIGALGTFVTGSDTNSNILFGKLQTTAAEKLHV
                     DPLWLAAANTSGATGGKMISPQSIAIAVSATRMDGQGSVIMSGTLKYCFAYIVILGLK
                     VGLVYHLFMA"
     gene            3561..4280
                     /locus_tag="RH033_004262"
     CDS             3561..4280
                     /locus_tag="RH033_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HDV2993358.1"
                     /translation="MNVNFFVTCIGDAFKSRMAQKSVLLLEQLGCNVHFPEKQGCCGQ
                     PAINGGYVRDAIPGMKNIISSLEENDWPIVSPAGSCTYTIKNYARYLSEEPGWAARAE
                     KVAERLHDLTSFIVNNLGITDVGASLNGRAVYHPSCSLSRKLGVTREPLLLLENVRGL
                     ELLPFHAQETCCGFGGTFSVKMAEISGEMVKEKVSHIMDVKPDFLIGADASCLLNIGG
                     RLSREGHPVKVMHIAEVLMHR"
     gene            4292..5707
                     /locus_tag="RH033_004263"
     CDS             4292..5707
                     /locus_tag="RH033_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HDV2993359.1"
                     /translation="MSLKTSDIRFKERIRIQIQDPIMRKAVANAQERIGTNRQKMVDE
                     LGHWEEWRSRAEQIRRHVLENLDAYLYQLSEKVTANGGHVYFARTREDATSYILKVAK
                     EKNARKVVKSKSMVTEEIGMNAVLQKAGIPVIETDLGEYILQLAHEPPSHVVVPAIHK
                     DRHQIRRVLHEHLGYDGPETPEAMTLFIREKIREDFLSAEVGVTGCNFAVAETGSVCL
                     VTNEGNARMCTTIPKTHIAVMGMERIAPTFQEVDVLITMLARSAVGARLTGYNTWLTG
                     PREEGHVDGPEEFHLVIVDNGRSEVLGSEFSEVLRCIRCGACINTCPAYRQIGGHGYG
                     SIYPGPIGAVISPLLGGYEDFKDLPYACSLCTACNSVCPVKIPLAKLIQKHRQAMAES
                     EITPLTERRITRVFNYVNSHPTLWKVGMVAGAKTASMFIKKGKALISVGAIKEWTEAR
                     DLPSPDGECFRSWFKKHKKEG"
     gene            5712..6410
                     /locus_tag="RH033_004264"
     CDS             5712..6410
                     /locus_tag="RH033_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HDV2993360.1"
                     /translation="MENREQFLVAIARKLGRPLRQTPEAMPEPVSTLATTRLTELTVD
                     QRCDAFIKFASEVMLAECVLVSPDEAASKALDICGKFGYGPVIISNDQRLVDTGIAPL
                     LQKEMGAVLWDPEKGEENIHIAEKAKTGVVFAEYGLTESGGVVLFSAPKRGRSVSLLP
                     EASLFVLRKSTILPRVAQLAQKLHEMAQEGQRMPSCINIIGGPSSTADIELIKVVGVH
                     GPVHATYLIIDDCD"
     gene            complement(6815..>7987)
                     /locus_tag="RH033_004265"
     CDS             complement(6815..>7987)
                     /locus_tag="RH033_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HDV2993361.1"
                     /translation="TIYIDKPLQKHNLIQTISRVNRKLEGKSKGLVVDYIGIKSQMNQ
                     ALAMYSRIDATNFEDIQQSVTEVKNHLDLLGQVFYDFDSRDYFSSEPQAQLSCLNRAA
                     EFVLRTQKVERRFMGLVKRMKAAYDVCCGSEALSQTERDHIHYYLAVRSIVFKLTKGD
                     APDVTQMNARVREMIAEALKADGVEEIYFLGDKKAESIDIFDEDYLARINKIKLPATK
                     IQLLQKLLEKAISDFRKVNQLQGINFTRRFQAIIDRYNERREDDVLNGEEFDTFSQEM
                     TDIIYDIKTEMGTWADLGIDIEEKAFFDILAHMRDKYQFTYDDEKMLSLAKEMKSVVD
                     NTSKYPDWSKRDDIKAKLKVELILLLHKHKFPPVANDDVYMGVLAQAENFKKNHMS"
BASE COUNT         2150 a         1739 c         1859 g         2239 t
ORIGIN      
        1 gaagtttctc tctccgaagc tcgtacaaaa cgggatgagg cccgaaaact gatttcagag
       61 gggattgacc cttgcgaaca gaaaagagtt aaaaaagttg tccctgattt acagctctct
      121 tttgaacata ttgcacgacg ctggcatgcc agtaataaac aatgggcaca atcacacagc
      181 gataaagtac tcaaaagcct cgagacacac gttttcccct ttatcggcaa ccgggatatc
      241 acaacactca ataccccgga cctgcttatc cctgttcgtg ctgcagaagc aaaacaaatt
      301 tatgaaatcg ccagtcgtct gcagcaaaga atatctgctg taatgcgtta cgccgtacag
      361 tctggcatca tcagatataa cccggctctg gatatggctg gtgcattgac cacggtaaaa
      421 cgccagcatc gccctgctct ggagctttca cgcctgcctg aacttctgtc gcgtattgac
      481 ggttataaag gccagcctgt cacccggctt gccgttatgc tgaatttact ggtttttatt
      541 cgttccagtg aactcagata tgcccgctgg tctgaaattg atattgacaa ttccatgtgg
      601 actattccag ccgaacgcga acctctgcca ggcgtaaaat tctcacaccg gggctccaag
      661 atgcgaacac cacatcttgt gccactcagc aaacaggctg tagccatact gacagaacta
      721 cagacatggg ctggtgaaaa tggtctgatc tttacgggag cacatgaccc gcgtaaacca
      781 atcagtgaaa atactgtaaa taaagccctg agggtgatgg ggtatgacac aacccaggat
      841 gtctgtggcc atggattccg ggcgatggcg tgcagtgcat tgattgaatc aggtttgtgg
      901 tcccgcgatg ctgtggaacg tcagatgagc catcaggagc gtaatggtgt acgtgctgcg
      961 tatatccata aagcagaaca tctggaagaa cggcgactga tgctacagtg gtgggcagat
     1021 tttctggatg cgaacagaga acaatgtatc agcccgtttg aatatgcaaa gattaacaat
     1081 ccattaaaat agtaatcatc ccgggctgcc agcccgggaa ttattttcaa tacttttttc
     1141 tttgagatta atgaataaac aaggcaattg acagaaccac tttcattacg tcatgtaatc
     1201 aaaattttta tagccgacat cagggatatc tgaacctcct ggtagcgggt aatttctctt
     1261 caggtatttt cgggtgctca actctggtta ttagcaaaat aatcgatata aacagtggaa
     1321 aattcatcgt acagaacatg gtgctcaaca ttactcttat tttcccttgc ccggaagaat
     1381 cacaggctgt gacttaaggc cgtccgcagg aactattttc tctccgttaa aaaccataac
     1441 agtttcatta tcttcattta tcagcagact gaacctctgc accgttgtta cgtaccagcg
     1501 tttgtttatc tattagagtc acttcagtag gcacatcaga tgccaacgca acattccctg
     1561 ccataagcgc agccagaacg cctgttacaa ctaaactatt cctggtgata ttgctcattc
     1621 tctttctcca tttgtcgagg atttgggcgt tgattttcga cgagctccag ttgcaactcc
     1681 agtcttaagg ttatatcggg atagcacaca agtgttacac gcataaacaa aaaataccca
     1741 aaaagtatta ttccataaca tttattcatg gaagataaca atgaaacgat tcagctatat
     1801 tttttaaact aaaaaatact ttataagaac atcccaattt tggaggcgat actcattctc
     1861 cccctgataa ctaaacgctc tcgtccgaca gggcgtggaa actatcaata accacagtaa
     1921 tgatgctgac tttgttttcg tttaaggaaa agacatgcac gattacctgc tttttttact
     1981 gggacttaca ccgcttctgg caatgatcat tttgattctg aaagtcaaaa tgcccataca
     2041 ttatgccgtg cttagcactc tcctgattac actaatcctt gggggagttt tctggcagac
     2101 gccagttatg gatatgggat ctgctgtggt atacggcgca atcaaaggtt tgtggccgat
     2161 tgtgattgtc atccttgccg ccatctacag ttataacctg atgaacgcga caaaaagcat
     2221 ggatgttctg agaaatgttc tggctagcgt gagcgatgat aaacgtatcc aggtgcttct
     2281 tatatcatgg tgctttggcg gatttcttga agcggcagct ggatacggta cagctgtggc
     2341 gatccccatt ggtatactga ttgctctggg ctttaaccca ctaaaagcag cgattgcgtc
     2401 actggttgct aatacagtcc ctacagcgtt tggggctgta ggcatacctg tctctgtgct
     2461 tgctgaacaa gttaatttgc ctgtaaccac actcagtggg acgattattc ttcagctggc
     2521 actgtttaac attctgctcc ccttcgtcat tgtcgctatt atcggtggtg gcatgaaggc
     2581 catacgaggc gttgggctaa ttaccctgat gtgcggtatt agcacattga tacctcagta
     2641 tctggtggct gttaatcttg gcgcagaact cccggctttt gcgggtagtc ttgtcagtct
     2701 gatcgttata gtctttatgg cgaaaaaccg taaagaaaaa acagagcctg aatttcttat
     2761 tgaggcttca gcagatagtg ccagcgcgct ttcatactct cgtggtgagt tatttcgtgc
     2821 gtgctcaatc tacatcctga tttttctgtt cattctgctc tgctctccgt tgttcccgac
     2881 gataaaaagt gcggtgtctc agattgcctc gtcactcagt ttctctctga gtaacggcag
     2941 caatctgtcg atgaagattg aatgggtagc aacccccggt gttctgatca tcattgccag
     3001 cgtgattggc gggttgattc agaaagcatc tctgtcatgc ctgttaaatg tgtttgtcag
     3061 cacggtaaaa cagcttaaaa actccattat tgccatttct gcgattgttg ccatggcaac
     3121 ggtaatggat gtttgtgggt tgattggcac tctggcacag agccttgtgg atctgactgg
     3181 tggggcctac cttttcattg ctccggttat tggcgctctt ggtacatttg ttactggcag
     3241 tgatacaaac tccaacattt tgttcggtaa attgcagacc actgcggctg aaaaattgca
     3301 tgtggacccg ctctggctgg ctgccgcaaa cacatctggc gcaacaggcg gcaaaatgat
     3361 ttctcctcag agtattgcta tcgctgtttc tgccacccgt atggatggtc agggcagtgt
     3421 aatcatgtca gggacactga agtactgctt tgcttatatt gttattctag gactgaaagt
     3481 aggtctggtt taccacctgt tcatggcctg ataaaaaaca acaaaaggac gtggaatatt
     3541 aatcaacagg agaaatttct gtgaatgtta actttttcgt cacctgcatt ggtgacgcct
     3601 ttaaatcccg gatggcacaa aaaagtgtgc tgttactgga gcaattgggt tgcaatgtcc
     3661 atttccccga aaaacaggga tgttgcgggc agccagcgat aaatggaggt tacgttcgtg
     3721 acgccattcc tggcatgaaa aacatcatat cttcgctgga agagaatgac tggcctattg
     3781 tatctcctgc tggttcatgc acctacacga taaaaaatta cgctcgctat ctcagtgaag
     3841 agcctggctg ggcggcacgc gctgaaaaag tggctgaacg actgcatgat ttgacatcct
     3901 tcattgttaa caatcttggg atcactgatg tgggggcaag tcttaatggt cgtgccgtgt
     3961 accacccttc ttgcagtctt tcccgtaagc tgggggtaac cagagaaccg cttttgctgc
     4021 ttgagaatgt acgaggactt gaactgcttc cgtttcacgc tcaggaaacg tgttgcggat
     4081 ttggcggcac gttctctgta aaaatggcgg agatctctgg tgaaatggtg aaggagaagg
     4141 ttagccatat tatggatgtt aagcctgatt tccttatcgg ggctgacgcc agttgtctgc
     4201 tgaacatcgg cggtcgactt agccgtgaag ggcatcctgt aaaagtgatg catattgctg
     4261 aagtcctgat gcacagatga ggtaaacaga aatgtctttg aaaacaagtg atatcaggtt
     4321 taaagagcgc atccgtattc agattcagga cccgatcatg cgaaaagctg tcgctaacgc
     4381 acaggagcgc atagggacta atcgccagaa aatggttgat gagctggggc actgggagga
     4441 gtggcgttca cgtgccgagc aaattcgtcg gcatgtgctg gaaaatcttg atgcctattt
     4501 gtatcaatta tccgaaaaag ttaccgcgaa tggcgggcat gtctattttg ctcgcaccag
     4561 agaggatgca accagttaca tactgaaggt ggcgaaagaa aaaaacgccc ggaaggttgt
     4621 taaatccaaa tcaatggtga ctgaggaaat cggcatgaac gccgttcttc agaaagccgg
     4681 gataccggtg attgaaacag accttgggga atatattctt cagcttgcac acgaaccgcc
     4741 atcacatgtg gttgtacctg caatacataa agatcgccat caaatccgca gagttttgca
     4801 tgaacatctc gggtatgacg gtccagaaac ccctgaagca atgacattgt tcattcgtga
     4861 gaaaataaga gaagattttc tgagtgctga agttggcgta acggggtgta actttgccgt
     4921 tgcagaaaca gggtcagttt gtcttgtgac aaacgaaggt aatgcacgta tgtgcacaac
     4981 tatacctaaa acacatattg ctgttatggg tatggagcga attgcgccaa cattccagga
     5041 ggtggatgtt cttatcacca tgctggcaag gagtgcagtc ggggcgagac tgacaggtta
     5101 taacacctgg cttactggcc caagagaaga agggcatgtt gacggccctg aagagttcca
     5161 tctcgtcatt gttgacaatg gacgttcaga agttctcggt tcagaattca gcgaggtatt
     5221 gcgctgtatc cgttgcggcg cttgcattaa tacctgcccg gcttatcgtc agatcggcgg
     5281 acatggttat ggttcaatat atccgggacc aatcggtgct gttatttcac cgttacttgg
     5341 tggatatgaa gactttaagg acctgcctta tgcctgctca ctgtgcactg cctgcaatag
     5401 tgtatgccct gtaaaaatac ctctggcgaa gctaatacaa aaacatcgtc aggctatggc
     5461 tgagtctgaa ataacaccat taacagaacg ccgtataacc agagtcttca attacgtaaa
     5521 cagccatcca acactctgga aggtgggtat ggtggctggc gcgaaaacag ccagtatgtt
     5581 tatcaaaaaa ggtaaagcgc taattagtgt tggggcaata aaagaatgga ctgaagccag
     5641 agaccttcca tcacctgatg gagagtgctt ccgtagttgg ttcaagaaac ataagaagga
     5701 ggggtaattt tatggaaaac agagagcaat ttctcgttgc gattgccaga aagcttggca
     5761 gaccactaag acaaacccca gaagctatgc ctgaacctgt aagcacactg gctacaacac
     5821 gtttgaccga gttaacagta gatcagcgct gtgatgcttt tattaaattt gcctctgaag
     5881 tgatgcttgc agagtgtgtt ctcgtgtcgc cagatgaagc tgcgtcaaaa gccttagaca
     5941 tctgtgggaa gttcggttac gggccagtga ttatcagcaa cgatcaacga ctggttgata
     6001 cgggaattgc gccattacta caaaaagaga tgggcgctgt tctgtgggat ccggaaaagg
     6061 gcgaagaaaa cattcatatt gctgaaaagg caaaaacagg tgtagttttc gctgagtacg
     6121 gcctgacaga gtcaggaggc gttgtcttat tctccgcacc aaaacgtggg cgctcagtaa
     6181 gtcttttgcc tgaagcttca ctgtttgttt tacgcaaaag taccattctc cccagagtgg
     6241 cgcagcttgc tcaaaaactt catgaaatgg ctcaggaagg acagcgaatg ccttcttgca
     6301 tcaatataat tggtgggcct agttcaaccg ctgatatcga gttgattaaa gttgttggag
     6361 tacatggtcc cgtacatgca acctatttaa ttattgatga ttgcgattaa cgattgctaa
     6421 taaaaaaatg gccttaaggt gatatttcgt cttgtgaaaa ggcaacaata gactaaaggt
     6481 taggcataat tgtgtttctt tatgcctccg cagcaatttg tggaggcttt tttgcttact
     6541 ccaaacagtt tggcttcttg aaaaagtcat atcccaaacc tattgacgct tataaaaaag
     6601 taaagaatgg tgagtaagcg taaactgacc accgtatgta gccatgatgc cagtattggt
     6661 aacgtaggtg cacccctatc tatttttgta gaaccggagg aaacagacca gatagtttaa
     6721 atgagctggt tatgcgtaac ataatttatc tattttggct cttacaaaat agacaaagaa
     6781 ggagtatctg atgagatact ccattatgac agactcaact catgtgattt ttcttaaagt
     6841 tctctgcttg cgccagtacc cccatataaa catcatcatt cgctactggc gggaacttat
     6901 gcttgtgtag aagcagaata agttcaactt tcagtttcgc tttaatatca tcgcgtttac
     6961 tccagtcagg atatttcgat gtgttgtcaa ccacgctttt catctctttt gccagcgaca
     7021 gcattttttc atcgtcatag gtgaactgat atttatcgcg catatgagca agaatgtcga
     7081 agaacgcttt ttcttcaata tcaataccta aatcggccca ggtgcccatt tcggttttaa
     7141 tatcatagat aatatcggtc atttcctgac tgaatgtatc gaattcttca ccgttgagta
     7201 catcatcttc tcgccgctca ttataacgat ctataatagc ctggaagcgg cgggtgaagt
     7261 taatcccttg caactggttc actttcctga agtcgctgat cgctttttcc agtaattttt
     7321 gtaatagctg gatcttcgtt gccggaagtt tgatcttgtt aattcgcgcc agataatctt
     7381 cgtcaaaaat atcgatggat tccgcttttt tatcgccaag aaaataaatt tcttctacgc
     7441 catcagcttt tagcgcttct gcaatcattt cacgaacgcg tgcattcatc tgggtaacat
     7501 ccggtgcgtc acctttcgtc agtttgaaaa caattgaacg aacagcaaga taatagtgaa
     7561 tatgatcacg ttctgtctgt gatagtgctt cactgccgca gcagacgtcg taggcggctt
     7621 tcatgcgttt aaccagtccc ataaaacgac gttcaacttt ctgggtacgc agaacgaatt
     7681 ccgccgcgcg gttgaggcag gataattgcg cttgtggctc actactaaaa taatcccgac
     7741 tgtcaaagtc gtaaaagact tgccccaaca aatcgagatg gtttttaact tctgtcaccg
     7801 attgctgaat atcttcaaag ttggtggcat caatgcggga atacattgcc agtgcctggt
     7861 tcatctgact tttaatgccg atgtagtcca ccactaaccc tttgcttttg ccttccagtt
     7921 tacggttaac gcgagaaata gtctggataa ggttatgttt ttgtaagggc ttatcaatat
     7981 agatggt
//
LOCUS       DAPHCV010000165         7951 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.167, whole genome shotgun sequence.
ACCESSION   DAPHCV010000165 DAPHCV010000000
VERSION     DAPHCV010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.167"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..74)
                     /locus_tag="RH033_004266"
     CDS             complement(<1..74)
                     /locus_tag="RH033_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDV2993362.1"
                     /translation="MELKWTSKALSDLARLYDFLVLAS"
     gene            complement(74..358)
                     /locus_tag="RH033_004267"
     CDS             complement(74..358)
                     /locus_tag="RH033_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HDV2993363.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWADSLSTDNPLPVPR"
     gene            complement(1263..2135)
                     /gene="repA"
                     /locus_tag="RH033_004268"
     CDS             complement(1263..2135)
                     /gene="repA"
                     /locus_tag="RH033_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HDV2993364.1"
                     /translation="MAALNQTSFTESTYRQVKNPNPVFTPREDAGTLKFCEKLMEKAV
                     GFTSRFDFAAHVAYARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTL
                     AIECGLATESEAGKLSITRATRALKFLSELGLITYQTEYDPTIGCNIPTDITFTPALF
                     SSLDISEEAVASARRSRVEWENKLRKKQGLDALGMDELIARAWRFVRERFRSYQAELK
                     SHGMKRARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIM
                     SRNNNYTRLATASP"
     gene            2838..3479
                     /locus_tag="RH033_004269"
     CDS             2838..3479
                     /locus_tag="RH033_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusG"
                     /protein_id="HDV2993365.1"
                     /translation="MCLSELQAGLNEGQPLFPTHALCIRRSQQHRSPSAGIDFRGAFR
                     ILNISELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILKKVRRTDKVCGYRR
                     HIHAVFPGYFFLKADPERHSFTHLRRHSAFLDFVKMAGEIKTVREDIVQSLMKVYPDP
                     ALNPAAREELDAASTLWLTKARYQYLLRLDAQPLPESRIALLLHLVSDDGALT"
     gene            3620..4285
                     /locus_tag="RH033_004270"
     CDS             3620..4285
                     /locus_tag="RH033_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HDV2993366.1"
                     /translation="MTILSQENPVILLLENMLEALSTAPDNINNERRRRRYLLNWLDT
                     ARQMREFRGMAEEFTTLRKLLATNSDIPVANTLKSLLESGNNAALYDLYRFRSALSAC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTRDTFSTTGRMLRPVAFSLIHP
                     ASLESSEVEEIFRDEGFILAHGRECSLNGSGRNMLSRILHVGHSGLPKAEWGIDHSNH
                     LLH"
     gene            4552..5172
                     /locus_tag="RH033_004271"
     CDS             4552..5172
                     /locus_tag="RH033_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HDV2993367.1"
                     /translation="MTLTLLTAYNAPYLAALTFVLLTGIAELIALLCGHSLSSAMDTP
                     DLPEGLTGEALDWLNIGRIPLLIVLCMLAGFFGISGILLQGLIIHLLQAPAPNILLAP
                     LCLLLTCPLVHRTGRLIAPWLPRDETTALTEEAFIGCMATITGYHAVAGSPCEGRFTD
                     RFGQVHYLLLEPEAGKEFRRGDKVIIVCRLSGSRWLGELNPWPDVL"
     gene            5199..6893
                     /locus_tag="RH033_004272"
     CDS             5199..6893
                     /locus_tag="RH033_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDV2993368.1"
                     /translation="MFNLPGWLFTVISLVLTLFVIGLIFARLYRRASAEQAFVRTGLG
                     GQKVVMSGGAIVMPIFHETIPVNMNTLKLEVSRAAAESLITRDRMRVDVAVAFFLRVK
                     PSAEGISTAAQTLGQRTLTPEDLRSLVEDKFVDALRATAARMSMQDLQDARENFVQGV
                     QNTVAEDLSKNGLELESVSLTSFNQTAREHFNPDNAFDAEGLTLLTQETERRRRERNE
                     VEQDVEVAIREKNRDALSRRLEIEQQEAFMTLEQQQRVKTRTAEQSASIAAIEAERRR
                     EAESARILAERKIEEAEIERQQIVRTRQVEAEREVAIREIEQQQATEIASQARAIAVA
                     AKSEEQSQAEARASKALAEAVQAQQDVETTRQTAEADRTKQVALIAAAQEAETKAVEL
                     TVRAQAEKEAAELQAAAIIELAEATRQKGLAEAEAQRALNDAINVLSDEQTSLRFRLA
                     LLDALPAVIEKSTEPMKSIDGIKIIQVDGLNGGVTRGKTATTDISGGNLAEQAVTAAL
                     AYRTQAPLIDSLLKEVGLSGEGLTALTRPLVMPAPEKVPDVSEPVTAPDNSEPQQV"
     gene            6899..7216
                     /locus_tag="RH033_004273"
     CDS             6899..7216
                     /locus_tag="RH033_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HDV2993369.1"
                     /translation="MPEFFRHLFHKECPHGIFWFKRGLKMSAEKFRVVIYTNTCEKKV
                     SYIPATEDINIAIMPFQTADNGLIIVCQNSRIIRRKERIPGSSCEWKDVTLAPKSRNR
                     VQL"
     gene            7333..7668
                     /locus_tag="RH033_004274"
     CDS             7333..7668
                     /locus_tag="RH033_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993370.1"
                     /translation="MRTITTHEQLLINGRVHERIATHIVTGAHGYETLCTNGYNVRYN
                     KAQQLVESCEKIAEGKLPVTCPVCFAIWQDVHRFTHVDFDTQSGKSDFIDTCHSEIIT
                     GNVSITGKK"
BASE COUNT         1950 a         2105 c         1983 g         1913 t
ORIGIN      
        1 ttactggcca gcaccagaaa atcatataac cgcgccaaat cagaaagcgc cttactggtc
       61 cacttcagtt ccatcagcgt ggcaccggta acggattgtc agtactgagg ctgtccgccc
      121 aggcctgtac agcctgatgg tcgataacct gtccggatgt cacatcgtcc agggcttcca
      181 gcgtcaggcg attacgctcc tcctcctggg caagccatgc agaaagcgcc tgtttgataa
      241 tccagccccg ggagcgttcc agtctggcgg ccatctggtc gactttatca gccataggta
      301 atggcacatg cgcggtaatt acttttgttg cctgtgcggt attgtttttc atttgcatat
      361 ccgattagct gttaatcata tttaatcata gtgattactt tcagacgaaa caaggcaaat
      421 aatgcgcgtc agcgcatgta aggcggcact ccgttgttac atatggcaca gaaacaaaac
      481 cgccgaagcc cggcgtcagc cggtactgat tgatagattt caccttaccc atccccagcc
      541 ctgctaagcc atacccgttt tcagccatga cagagcttct gtgcgcggtc ggagtggtcc
      601 cgacgagggt ttacccgaag tcggggcgta tctccgcgtt agcgggccgt cagggccgct
      661 tacgagcgtg tactgaggac ttccagccag aagactgaca gcgatgagga ttcttagtta
      721 caacattcat aattaaaagc gatgttgttc ccgcccttcg ggcgggggcg gcctcgcttt
      781 tcagttatga gaggtgagca cggtgctgcg tttgtgtgat gtttttattc ctgatgtctt
      841 tttgctgtgg tttcagaacg gtgaggttcg gggcccgcgc gttagcgtgg gaggtgcata
      901 tttgcttgtc cacaggacag gcactgaata ggttttcttt tcaaatggaa gtaattaagt
      961 agtttaaagg aaatacagta ctggggttta aaagaaacat tgcaccctgt aacgcctgcg
     1021 gctggcgctt tatagcatta acggttgtaa ccttacggag aagtcccttg cagttaaatg
     1081 tggataaaca gaattccccg tctgtgagac gtgttttgcg tgaaaaacag ggggagcaag
     1141 gcgagccgga aggcagatga tgaggctgtt ttttgtgtgc tgtgctgctt ttttgtgcgt
     1201 cgccgggctt cagacgtgcg gaagcctccg gcgcaggccg gattattcag atgaggtcaa
     1261 actcagggtg aggcagtggc cagccgtgtg tagttgttgt tccgcgacat tatcatgcgt
     1321 tccttcacgc gacggtcgat ttcgcgcttc acggcctcca ggcttccgcg gaaacgccct
     1381 tccgcgattt cacgtgtcag ctgccgtttc accagggtga caatgtcctg acgggtcctg
     1441 cctgcatcgc ggcgcgcacg ggcacgtttc atgccgtggg acttaagctc tgcctgataa
     1501 ctacggaaac gctcgcgcac aaaacgccag gctctcgcta tcagttcatc catacccagc
     1561 gcatccagcc cctgcttttt gcgcagcttg ttttcccatt ccacgcggct gcggcgtgcg
     1621 gaagccaccg cctcttctga tatatcgagt gaggaaaaca gtgccggcgt gaacgtgatg
     1681 tcggtcggga tgttacagcc aattgtcggg tcgtattccg tctggtaggt tatcagcccc
     1741 agctcagaca ggaacttcag ggcacgggtg gcacgggtga tggagagttt cccggcctca
     1801 gactccgtcg ccagtccgca ctcaatggcc agcgtggtga tggagcactg gacgcggttg
     1861 gccagcggat catagtggaa acacagcccc tgcagcagcg catcaatcgc ccggcggcgc
     1921 agcaccggcg gcatacgccg gcgcaggccg cgcgaacggg catacgccac atgcgcggca
     1981 aaatcaaaac gggaggtgaa gcccaccgcc ttttccatca gtttttcgca gaacttcagc
     2041 gttccggcat cttcacgggg tgtgaacacc ggattcgggt tctttacctg acggtatgtg
     2101 ctttcagtaa atgaggtctg gttcagggcc gccacaagct gtcgcttatg gcggaaacag
     2161 aatgccgtgc gttaatacac tgcgaagggt tgaatctctg acacggtttc atatatactt
     2221 atcccgtatt ctgtgaggcc cccataatct tctgtgcgtt ccgccaagtt cgacaggaag
     2281 attatggggg ttttccttta tggccttctg aaactttttt cagccttccg gccccggcaa
     2341 actgccgtcg tttctgccat acatcctgaa tgccttatgg cgcattctgc cgttttctgg
     2401 ctttccggac cgcgaggatc ccaaaagcca ctgaataacg atccgcaata tacccctttt
     2461 ccaccgcttt gaaaagccga cacgatttct ctgatttacc aggcctctca ttaaccacct
     2521 aaaaaacgtc gtttttttga actatgtcaa aaaaatagac aattccacaa acgatctttt
     2581 atgctgtgtt tttcatcatt tttagtagaa gagatcgcct aaaattttta aacgatctaa
     2641 tcctccagac accgcacaca aatcagcata atatgctgct ttatcaaata taacgatcta
     2701 tttaactgct tatcttcact cttgccacag atacatcctt atgtaatata tttgccagcc
     2761 attaatcctg tttgcaggag ttgtcggttt tcgcccgcct cgaaaccgtc cacaaggctt
     2821 acaagagtgc gtaaatcatg tgcctgtcag aactgcaggc cgggctgaac gagggtcagc
     2881 cgctgtttcc gacacatgct ttatgtattc gccgttcaca acaacatcga agcccctctg
     2941 cggggatcga ttttcgcgga gcgttccgga ttttgaatat cagtgaatta catcaacgca
     3001 actggtatct ggctcagtac atccctgccg gcaaaaaccg cgaacatctc ttttcctggc
     3061 tttccgaaca acacgtcctt ccctggacac ccctgatact gaaaaaggtc cgccgcacgg
     3121 ataaagtctg tggttaccgt cgacatatac acgccgtgtt tcccggttac tttttcctga
     3181 aagcagaccc cgaacgccac agcttcactc accttcgccg tcacagtgcc tttctggact
     3241 tcgtgaaaat ggccggtgaa atcaaaaccg tcagggagga tatcgttcag tctttgatga
     3301 aagtgtaccc ggatcctgcg ctcaatccgg cagctaggga ggagctggat gctgcctcca
     3361 ccctctggct gacaaaagcg cgttaccagt acctcctccg gcttgatgca cagcctctgc
     3421 cggagtcgag gatcgccctg ctcctgcacc tggtatctga tgacggagcg ttgacatgaa
     3481 aattgctgtc ttaatttgac gacttttccc ggcctttatg gccgggattt ttttcccttc
     3541 tgtttccaca atgcccggca ccggtgacac ctgagggtgt acggagagcg gctgtcgggc
     3601 attttttcag gacgccttta tgaccattct atcccaggaa aaccctgtca ttctgttact
     3661 ggagaatatg ctcgaagcac tctccaccgc cccggacaac ataaacaacg aacggcgtcg
     3721 ccggcgttac ctgctgaact ggcttgacac tgcccgtcag atgcgcgagt tcagaggaat
     3781 ggctgaagag ttcactaccc tgcgaaaact gctggccacc aattcagaca taccggtagc
     3841 aaacaccctg aaatcactgc tggaaagtgg caataacgca gcattatacg acctgtaccg
     3901 cttccgctcg gctctttccg cgtgcagaag tctgggatgg cagattggca cgtgcgcatg
     3961 gaacgaccag ctgctgagtg agacactttc ccgtgcgcaa acaggtaaac gtcatatcct
     4021 gcagctgaac agtaccagag acaccttttc caccacgggc agaatgctca gaccggtggc
     4081 attttcatta attcatccgg catccctcga atcttcagaa gtggaagaga tattccggga
     4141 tgaaggtttt atactcgcgc acggacgcga atgcagcctg aacggcagcg gcagaaacat
     4201 gctgtcacgc attcttcatg tggggcattc aggtttaccg aaagccgaat ggggcataga
     4261 tcacagcaat cacctgttac actgaaatac cgggacagcc acaacggcag ccactctccg
     4321 ctgacttttt ttcgcaaatc gcggtttgct gatgtggatg tcccattttt tattatccgt
     4381 cctctccggt tgtcataaag cgcattccgg cgtgaggctt ctgttgttat catcccctcc
     4441 tgcagacatc cccttgtctt ctgatgactg acgatatgct tcttctgaca attaaagaag
     4501 cggaagcttc acgccataat gttgtttgac tgacctgcca caggaggaaa aatgacactg
     4561 acgttactta ccgcgtataa cgcaccctat ctggcagccc tgacttttgt tctgcttact
     4621 ggtatcgctg aacttatcgc cctgctctgc ggacactccc tctcttcagc tatggataca
     4681 cctgatttac cggaggggct gacaggagag gcgctggact ggctgaacat cggacggatc
     4741 cccctgctga tcgtcctctg tatgctggca ggtttttttg gtatatccgg cattctcctg
     4801 cagggtctta ttattcatct gctgcaggct cctgccccga acattctcct tgcaccactt
     4861 tgcctgctgc tgacatgtcc tctcgtacac cgcaccggca gactgattgc cccctggtta
     4921 ccccgtgatg aaacaacggc gctcaccgag gaggcattca tcgggtgtat ggccaccata
     4981 accggttatc acgcagtggc gggttccccc tgtgaaggtc gcttcacaga ccgctttggg
     5041 caggttcatt acctgttgct ggagccggag gccgggaaag aattcagacg aggagacaaa
     5101 gtgattattg tctgccgtct ttccggttcc cgctggctgg gcgaactgaa tccctggccg
     5161 gatgttcttt aatcccgaca tatacagagg ttctcactat gtttaatctt ccgggatggt
     5221 tgtttaccgt tatttcgctg gtcctcaccc tgttcgttat cgggctgata tttgcccgcc
     5281 tgtaccgtcg ggcatcggca gagcaggcct ttgtccgcac aggtctggga ggccaaaaag
     5341 tggtaatgag tggcggtgca attgttatgc ccatttttca cgaaacaatt cccgtcaaca
     5401 tgaacaccct gaagctggaa gtcagtcgtg cagcggctga aagtctcatt acccgcgacc
     5461 gcatgcgtgt tgatgttgcc gttgcctttt ttcttcgggt aaaaccatca gccgaaggaa
     5521 tatccactgc agcccagacg ctgggacaac gcacgctcac acctgaagat ctgcgtagtc
     5581 tggtcgagga taaatttgtt gatgccctgc gggccacggc ggccagaatg agcatgcagg
     5641 atttacagga tgcccgcgag aactttgttc agggtgtgca gaacaccgtt gcggaagact
     5701 tatccaaaaa cggtctggag cttgaaagtg tgtcgctgac aagtttcaac cagactgcac
     5761 gggaacattt taatcctgac aatgcctttg atgctgaagg cctgaccctt ctgacccagg
     5821 aaacagaacg tcgtcgccgc gaacgtaacg aagttgagca ggatgttgaa gtggccatca
     5881 gggaaaaaaa ccgggatgca ctctcccgac gtctggagat tgaacagcag gaagccttca
     5941 tgactctgga gcaacaacaa cgggtaaaaa cccgtacggc agagcagagt gccagtattg
     6001 ctgctattga agctgaacga cgtcgtgaag cagaatccgc gagaattctg gctgaacgta
     6061 agatagagga agccgaaatc gaacgacagc agatagtgcg tacccgccag gtcgaagccg
     6121 aacgggaagt ggccatacgg gaaattgagc aacagcaggc aaccgaaatc gccagccagg
     6181 ccagagccat tgccgtggct gcaaaatcgg aagagcagtc acaggcagaa gcccgggcaa
     6241 gtaaagcact ggctgaagcc gtacaggccc agcaggatgt ggaaaccacc cgccagactg
     6301 cggaggccga ccgtaccaaa caggttgccc tgattgctgc tgctcaggaa gctgaaacga
     6361 aagcggttga actgacggtt cgcgcacagg ctgaaaaaga agcagccgaa ctccaggcag
     6421 cagcaattat tgaactggcg gaggccaccc gtcagaaagg tctggcagaa gcagaagcac
     6481 agcgcgcact gaacgatgcc attaacgtgc tttcggatga gcagaccagc ctccgcttcc
     6541 gtcttgccct gctggatgca ttacctgccg tcatagagaa gtccacagaa ccaatgaaat
     6601 ccattgacgg cattaaaatc attcaggtgg atggcctgaa tggcggtgtt acccgtggta
     6661 aaacagcaac aaccgacata agtggcggaa atctcgctga acaggcggta accgccgctc
     6721 tggcttaccg tacacaggct ccgctcatcg actccctgct gaaggaggtg gggttatccg
     6781 gtgaaggact tacagccctg acacgtccac tggtcatgcc agccccggaa aaggtcccgg
     6841 atgtcagcga accggtaacc gctccggata actcagaacc acaacaggtt taatccccgt
     6901 gccggaattt ttccggcatc tttttcacaa agaatgccct cacgggatat tctggtttaa
     6961 aagaggattg aaaatgtccg cagaaaaatt cagagttgtc atatacacca atacatgtga
     7021 aaagaaagtc agttatattc ctgcaacaga agatataaac atcgctataa tgccatttca
     7081 gacggcagac aatggcttaa ttattgtgtg tcagaactcc cgtataatac gacgcaaaga
     7141 gcgtataccc ggaagcagct gtgaatggaa agatgtgaca ttagcaccaa aatccagaaa
     7201 ccgcgttcag ttatagcctg acagcctgct ttttatccct cagaaaaggg ggataaaaat
     7261 taaatatttc acaaccgaga tatttttcag gaatgcagat tattttatgt tatgaccaac
     7321 agagaaacag gcatgagaac aatcacaacg catgaacaat tattaattaa tgggagagtc
     7381 cacgaaagaa tcgccacaca tatagttaca ggagctcatg ggtatgaaac gctttgcaca
     7441 aatggctata acgtcagata caacaaggcg cagcaactgg ttgaaagctg cgaaaaaata
     7501 gctgaaggca aactacctgt tacctgtcct gtctgtttcg ccatatggca ggatgtacac
     7561 aggttcaccc acgttgactt tgacacacaa tcggggaaaa gtgattttat tgatacctgc
     7621 cattcggaga tcataaccgg aaatgtttca ataacaggca agaaataaaa taatgctcca
     7681 gttctgaaat gcaattcact cttataatcc ccccacgaac atgaacgttc attatcaata
     7741 acccgatgaa atgatattca tctgaaggcc agttacccgc cacctgtctt ataccgtaac
     7801 ccgtaatata ccggatttta ttctatgcaa aaaaaatcac ttacactcct tctgccgtca
     7861 ttactcagcg gctgtacggc atttaatgcc acgcctgttc ctgacaccac acctccggct
     7921 ctggtgttcg tcgacggaca aattcgcgaa a
//
LOCUS       DAPHCV010000166         7656 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.168, whole genome shotgun sequence.
ACCESSION   DAPHCV010000166 DAPHCV010000000
VERSION     DAPHCV010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.168"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            314..1528
                     /locus_tag="RH033_004275"
     CDS             314..1528
                     /locus_tag="RH033_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993371.1"
                     /translation="MPIRLNTIPDAEPYPAPPARIKWLSALLLMLSVGVAITSLFSSD
                     ELAKNGPHFWGLACGVPAFIWSLVASVRWLFFITQYIRADAWNKRREEVILQETRRGR
                     RALQILSFSVQTALNGDSVAETTTAFLARQQVLNTYADLRGEDTVRRSVIPVLTNKPV
                     TGRLSEIISRLFIDIRPQLFLLPPDFHINVLLEIDAPLSGASVRTIWQDEWQKAGLPE
                     ARLFSAPELGLAAVDDWLDNFIQEKAVLLVISVRLEPKTPERTAESSTALLLANRLTQ
                     TALTPLALLHRPERITDTEMMASGIAQALDWMPVQPDAISGIWTAELDREQRAALLSL
                     NQPFIQEALMYELDAFLGRSGPAAPWLSVAAATLAAIQSQHPQLTLSGVQGGHYSWAT
                     VVSPFVSPQEAS"
     gene            1585..4911
                     /locus_tag="RH033_004276"
     CDS             1585..4911
                     /locus_tag="RH033_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein VasK"
                     /protein_id="HDV2993372.1"
                     /translation="MTVAVVVSLCTFIHRKPALLGVEPGSSQQMYWMAGVIAAGVVFL
                     IIMGVYHLLNRHAAVQSFELHRQLAAGDDYRAVTTKKADSRHPAMVELAAFLRDRYGP
                     FWRRKIRLLLVTGEPEQAEAIAPGLTGQHWLEGDHTVLIYGGRPSTEPDVALLTALKK
                     LRRSRPLDGIIWPLTEEQSRQTAQLDKGWRELINGGKRLGFQAPLYLWQVCDNGDYQT
                     GRPLQSVGCLLSERCTPEQLAAMLEAQTLPLTEQGMSQLLTDNRHDFLLRLAHTLAER
                     GIAHWQSILKPLLAGGAFSSLRLRGLMFSPPLTAVPEAAPHAWLPSPVWAGVTGDNAR
                     GRTVGFPWLRITLMSAVCVLAIWGAGMTTSFFANRALIQETSIQTARALDTRLPVSEQ
                     LVALHTLQGELERLQYRIREGAPWYQRFGPERNQQLLAAAFPGYAQAANRLVRDVAVD
                     HLQQQLNAFVALPPNSPQRTATGEQRYKQLKALLMTSRPEKADAAFFSTTLMADGLRY
                     ENIPEGVRQSVLPSLLTFWMANLPEHPQWKTSPPPELTGAVRKILLRQIGVRNAENTL
                     YQNVLQQVSRNYADMTLADMTGDTLTESLFSTEQTVPGMFTRQAWEGQVREAIEQVVT
                     ARREEIDWVLSDRQQDTSADISPDTLRNRLTSRYFTDFAGSWLAFLNSIHWKKEDSLS
                     GILDQLTLMADARQSPLIALTDTLAWQAAAGRENRGLSDSLAKSAQELFNGKEKTPQQ
                     SREGDVPVGPLDKTFAPLLRLLGDKAGGGDSQLSLQTYLTRVTRVRLKLQQVTNAPDP
                     QEMTQQLAQTVLQGKTVDLTDTRDYGRLIAASLGEEWSGFGQALFVRPVEQSWRQVLT
                     PAADSLNRQWQRAIVSHWNQDFAGRYPFKASQNDASLPLLAQYLRDDGRINQFIAANL
                     SGVLKREGRYWVADAMNTQGLTVNPDFIRALNRLRDVADTAFASGDAGIHFELRAKPA
                     RDVMKTHLVIDGQELEYFNQKERWQRFNWPDEQWQPGASLSWTSTQAMERILADYRGS
                     WSLIRLLEQAQVTPVDSSTFKVEWKAQDGLPLNYLLRVEQGKGPMALLELKNFRLPGQ
                     VFLTGRSMKDAEEYGEDADE"
     gene            4904..6514
                     /gene="tssA"
                     /locus_tag="RH033_004277"
     CDS             4904..6514
                     /gene="tssA"
                     /locus_tag="RH033_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HDV2993373.1"
                     /translation="MSELLQKLTRSCFADRDALDSARTQAALWQTWLLPVTADTPVGE
                     DPGYHDDFLRIRDEMNKLSGADTDLICQLAESLLLTQAKDVRIATYYIWARLHRDGER
                     GLAEGLALLAGLVERFGAQLLPSRPASRKMALEWLAGEKMLDSLARYPEVAKEDFANI
                     VAALNQLTVSFTAWPEDQHSPSLMPLINALESRLAQSGGMNAVVPQNSSGVPAPSSPV
                     DAPQVQTITSGRDLLDQAKVLARYLNEQPQGWLSAHRLMKTLRWDTVHELPPDVDGKT
                     RLAPPRTESRNQLKRLYAQQNWTELLEQADLMFSSGVSHFWLDIQWYLHQALAKAGAP
                     WDRWTAVIRQDLALLLERLPGLENLAWNDGTPFADEVTRNWIAQQVMMREDGAWLAGK
                     AAVPTDDATNDVLALEPEALEIADSQGVEAALGWIQTRPGITTARQRLLLRLLMARVA
                     EQYGKNEMALLLLEELDTAAQGITLTQWEPELLFEVKARQLKLLRLRAHRYADKALLN
                     RKMDALLGTLVAINPVRAAVLCDTQHKD"
     gene            6520..7656
                     /locus_tag="RH033_004278"
                     /pseudo
     CDS             6520..7656
                     /locus_tag="RH033_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402492.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
BASE COUNT         1788 a         2022 c         2270 g         1576 t
ORIGIN      
        1 tgatgattat ggcgaaataa acagaatgta ttattggtta ccttgtgaaa ttagagggag
       61 actaatgtat gacggaatag aatggagttt tgttatcaat gctgctgcaa cagcaatgtg
      121 gatgagcaat gataaagaga tttactgggg atgcaataaa aaagcatgcg ataatttctt
      181 tttattacca tatgatggga tgaggtaaat cacagttaat aaagcaaaaa cattatggac
      241 aagtgaatta atcagatagt tcaagcacct tgagattcat tgtattcata tttcagaata
      301 acggattatt acaatgccca tccgacttaa taccattccc gacgcagaac cctatcccgc
      361 accgccagcc cgcattaaat ggctgtcagc cttattactg atgctgtctg ttggtgtggc
      421 aataacctca ttattctcca gcgatgaact ggcaaaaaac ggcccgcatt tctggggact
      481 ggcctgtggt gtgcctgcct ttatctggtc gctggttgct tcagttcgct ggctgttttt
      541 tatcacgcag tacattcggg ctgatgcctg gaataagcgc cgggaagagg tgattttgca
      601 ggaaacgcga cggggacgac gggcgttgca gatactcagt ttttccgtac aaaccgcgct
      661 gaatggtgat tccgttgcag aaactacaac agcatttctg gcgcgtcagc aggtgttgaa
      721 tacttacgct gatttgcgcg gagaagatac tgtgcgtcgc agtgtcattc ccgtattaac
      781 caataaaccg gttaccgggc gtttatccga aattatttcc cgccttttta ttgatatcag
      841 accgcaattg tttctgctgc cccctgattt tcatatcaac gtactactgg aaattgatgc
      901 gccgctttca ggggcctccg tgagaactat ctggcaggac gaatggcaaa aagccggact
      961 cccggaagcc cgtctgtttt ctgccccgga gctggggctg gccgccgtgg acgactggct
     1021 ggacaacttt attcaggaaa aggccgtact gttggtgatt tccgtacgac ttgaaccgaa
     1081 gactccggaa cggacggcag aatcctccac cgcgctgttg ctggcaaatc ggttgacgca
     1141 aaccgcgctg acgccgctgg ccctgctgca tcgtcctgaa cgtatcacag atacagagat
     1201 gatggcgtca ggcattgcac aggcgctgga ctggatgcca gtgcagccag atgccatatc
     1261 cggcatatgg actgccgaac tggacaggga acagcgtgcc gcgttgttaa gccttaacca
     1321 gccgtttatt caggaagccc tgatgtacga gctggatgcc tttctggggc gctccggacc
     1381 ggcagcgccg tggctgtccg tcgctgcggc aacgctggcg gcaatacaat cacaacaccc
     1441 ccaactgacg ctcagcggcg ttcagggcgg gcattattca tgggcgaccg tggtgtcgcc
     1501 attcgtttcc ccacaggagg catcatagtg gttccgggat taaaccgtct ccggccccgt
     1561 tctgtttcag ggcctgccat cgtcatgacc gtggctgttg tggtttcgct ttgcacgttt
     1621 attcaccgta aaccggcatt gctgggcgtt gaaccgggta gttcacagca aatgtactgg
     1681 atggctggcg tgattgccgc aggtgtggtc tttctgatta tcatgggggt ttatcatctg
     1741 ctgaaccgcc acgctgccgt acagagcttt gaactccaca gacagcttgc cgccggagat
     1801 gactatcggg cggtgacgac aaagaaagcc gacagccgtc atccggcaat ggtggaactc
     1861 gccgccttcc tgcgcgaccg ctacggccca ttctggcggc gtaaaatccg cctgctgctg
     1921 gtgaccggcg agcctgaaca ggcagaggcc atcgcgccgg ggctgaccgg gcaacactgg
     1981 ctggagggcg accacacggt gctgatatat ggcggcaggc catctacaga gcctgatgtc
     2041 gcgctactga ccgccttaaa aaaactgcgc cgcagccgtc cgctggacgg catcatctgg
     2101 ccgctgacag aagaacagag ccgccagacg gcgcaactcg acaaaggctg gcgcgaactg
     2161 ataaacggcg gtaagcgact cggttttcag gctccactct atttgtggca ggtctgtgac
     2221 aacggtgatt atcagaccgg acgccccctg caaagcgtcg gctgcctgct gtcggaacgc
     2281 tgtaccccgg aacaactggc tgcaatgctg gaagcacaaa ccctgccgct gacggaacag
     2341 ggcatgtcgc agctgcttac cgataaccgc catgatttcc tgctgcgtct ggcccatacc
     2401 cttgcagagc ggggtatcgc tcactggcaa agtatcctga aaccgctgct ggcaggcggc
     2461 gcattttcct ccctgcgcct gcgtggcctg atgttcagcc cgccgcttac cgccgtaccg
     2521 gaagccgcac ctcatgcgtg gctgccgtca ccggtctggg cgggcgtgac cggcgataac
     2581 gcgcggggtc gcacggtggg ctttccgtgg ctgcgtatca cgctgatgtc cgctgtctgc
     2641 gtactggcga tatggggggc cggaatgacg acctcgttct tcgccaaccg cgccctgatt
     2701 caggaaacca gcatccagac ggcacgggcg ctcgataccc gtctgccggt ttcagagcaa
     2761 ctggtagcgc tgcatacctt acagggtgaa ctggagcgcc tgcaataccg tatccgggag
     2821 ggtgcgccgt ggtatcagcg cttcggccct gaacgtaacc agcaactgct cgctgccgct
     2881 tttcccggct atgcgcaggc ggcaaaccgg ctggtgcgcg acgtggccgt tgaccatctg
     2941 caacagcaac ttaatgcctt tgtcgcgctg ccgcccaaca gtcctcagcg taccgccacc
     3001 ggtgaacaac gctataagca gcttaaggca ttgctgatga cttctcgccc ggaaaaggcc
     3061 gacgctgcct ttttcagtac cacgctgatg gcagacggcc tgcgctatga aaatatcccg
     3121 gaaggcgtgc ggcagagcgt gttgccgtca ctgctgacct tctggatggc gaacctgccg
     3181 gaacacccgc agtggaaaac ctcaccgcca ccggaactga ccggcgcggt gcgtaaaatc
     3241 ctgctgcgcc agattggcgt gcgcaatgcc gaaaacaccc tctaccagaa cgtgctgcaa
     3301 caggtgtccc gcaactacgc cgatatgacg ctggcggata tgaccgggga tacgctcacc
     3361 gaatctcttt tcagcacgga acagacggtg ccggggatgt tcacccgtca ggcgtgggaa
     3421 ggacaggtca gggaagccat cgagcaggta gtgacggcgc ggcgcgagga aatcgactgg
     3481 gtactcagcg accgccagca ggatacctct gcggatatct cgccggatac gctgcgtaac
     3541 cgtctcacct cacgctactt taccgacttt gccggaagct ggctggcgtt tctcaacagc
     3601 attcactgga aaaaggaaga ctcgctctcc ggcattctcg accagttgac actgatggcc
     3661 gatgcccgtc agtcgccact gattgcgctg acggacaccc tcgcgtggca ggcggcggca
     3721 ggcagggaaa accgtggtct gtcagactcg ctggcgaaat cggcacagga actgtttaac
     3781 ggcaaggaga aaacgccgca gcaatcccgt gaaggtgacg tgcctgtcgg gccgctggat
     3841 aaaaccttcg ctccgctgct gcggttgctg ggcgataagg ccggaggcgg cgacagccaa
     3901 ctgagcctgc agacctacct cacccgcgtc acccgtgtgc gtctcaaact gcaacaggtg
     3961 accaacgccc ccgacccaca ggagatgacc cagcaactgg cgcagacggt tttacagggc
     4021 aaaaccgttg acctcaccga cacccgcgac tacggacggt taatcgccgc cagtctgggc
     4081 gaggaatgga gtggcttcgg tcaggcgctg ttcgtccgcc cggtggaaca gtcgtggcgg
     4141 caggtgctga cgcctgcggc ggacagcctg aaccgccagt ggcagcgggc gattgtcagc
     4201 cactggaatc aggacttcgc cggtcgctat ccgttcaaag cctcacaaaa cgatgcctct
     4261 cttcccctgc tggcgcagta tctgcgcgat gacgggcgca tcaaccagtt tatagccgcc
     4321 aacctttccg gcgtactgaa acgtgaaggc cgctactggg tggctgacgc catgaacacg
     4381 caggggctga cggttaaccc ggactttatc cgcgccctga accgcctgcg cgacgtggcc
     4441 gataccgcct ttgccagcgg cgatgccgga atacattttg aactgcgggc aaaaccggcg
     4501 cgtgacgtga tgaaaacgca tctggtgatt gacggacagg agctggaata tttcaaccag
     4561 aaagaacgct ggcagcgttt taactggccg gatgaacagt ggcaacccgg cgcatcgctg
     4621 agctggacca gcactcaggc aatggaacga atactggcgg attaccgggg aagctggagc
     4681 cttattcgtc tgctggaaca ggcgcaggtg acaccggtgg acagcagcac ctttaaagtg
     4741 gagtggaaag cgcaggacgg cctgccgctg aattacctgc tgcgggttga acagggcaaa
     4801 gggccgatgg cgctgctgga actgaaaaac ttccgcctgc cgggacaggt gttcctgacc
     4861 ggcaggagta tgaaggatgc ggaagaatac ggggaagacg ccgatgagtg aactgttgca
     4921 aaagctgacc cgtagctgtt ttgccgaccg ggacgcactg gactcggcgc gaacacaggc
     4981 ggcgctgtgg cagacatggc tgctgccggt gacggcagat acgccggtcg gggaagaccc
     5041 cggctaccac gacgatttcc tgcgtatccg cgacgagatg aacaaacttt ccggcgcgga
     5101 tacggacctt atctgccagc ttgccgaatc cctgctgctg acgcaggcaa aagacgtgcg
     5161 catcgccact tactatatct gggcgcgact gcaccgcgat ggcgaacgcg gactggctga
     5221 ggggctggcg ctgttagccg gactggtgga acgcttcggt gcgcaactgc tgccctcgcg
     5281 cccggcaagc cgaaaaatgg cgctggagtg gctggcaggg gaaaagatgc tcgacagcct
     5341 tgcccgctac ccggaagtgg caaaagagga ttttgcgaac attgttgccg ctcttaatca
     5401 actgacggtt tccttcactg catggcccga agaccagcat tccccctccc tgatgccgct
     5461 gattaacgcg ctggaatcac gtctggcgca gtcgggtggg atgaacgcgg tggtgccgca
     5521 gaacagcagc ggcgttccgg caccgtcttc cccggtggat gcgccacagg tacagaccat
     5581 cacttccggg cgcgacctgc tcgaccaggc gaaagtgctg gcccgttatc tgaacgaaca
     5641 gccgcagggc tggctttcag cacaccggct gatgaaaacc ctgcgctggg acacggtgca
     5701 tgaactgccg ccggatgtgg acggaaaaac ccggctggcc ccgccgcgca cagaatcacg
     5761 caaccagctt aagcggctgt atgctcagca gaactggacg gaactgctgg agcaggccga
     5821 cctgatgttc agctccggcg tcagccattt ctggctggat atccagtggt atctgcatca
     5881 ggcgctggcc aaggcgggcg caccgtggga cagatggaca gcggttatcc gtcaggacct
     5941 ggcgctgctg ctggagcgcc tgccggggct ggaaaatctg gcatggaacg acggcacgcc
     6001 gttcgccgac gaggtgacgc gcaactggat agcgcagcag gtcatgatgc gggaagacgg
     6061 cgcatggctt gcggggaaag ccgctgtacc gacagacgac gccacaaacg acgtactggc
     6121 gctggagccg gaagcgctgg agatagcaga cagtcagggc gtggaagcgg cgctgggctg
     6181 gatacagacc cgtccgggca tcaccacggc acgacagcgc ctgctattgc gactgctgat
     6241 ggcgcgggtg gcggaacagt acggtaaaaa cgagatggcg ctcctgctgc tggaagaact
     6301 ggatacagcc gcacagggaa taacgctgac gcagtgggag ccagaactgc tgtttgaagt
     6361 aaaagcccga cagctgaaac tgctgcgtct gcgggcgcac cgctatgcgg ataaagccct
     6421 gctgaaccga aaaatggatg ccctgctggg tacactggtg gcaatcaatc cggtgcgggc
     6481 agcggtattg tgtgatacgc aacataagga ctgatggcaa tgggaacagg ctacttcctg
     6541 gtcaggggcg ataagactac ctgcggcggc aaaattattg aaggcgcgga cgaccatacg
     6601 atcatgggta tcccgcaggc acgggatatg gaccgggtca cctgcggcag atatccggga
     6661 atgtttatta ttgtgggcgg cgttcctgaa acagatattc acggcaggct gatggcgggg
     6721 acgctggata gccagagcag ttgcccgtgt aaggcgcggt ttattgcgtc gatgatggat
     6781 gatacctatg aggtggatga cgggggaagt gaagcggaac aacatgcgca gtcggcgaaa
     6841 aaggatctga cttctggttc tgattcatcc tctgatgatg taaaactgga ttacagaatt
     6901 aaattatcag gtaataagat actgacgcca ctgaatatac ctgattataa agaaatgata
     6961 agcggtggta gtacgaagaa tacagaaaaa aatagatttt actatcacaa ataaaggcga
     7021 tgaggctgaa gcgctttctc tggaagtgct tgatggtaat gaagtgattt atagtgaacg
     7081 gcagaccggt aaatattgtg ataagggaga gcatgcctgg caatgggatg gttacagtaa
     7141 tcaaggcata ctggatacaa ctaagataaa aagcaaatca ctactcgtaa gactgattgc
     7201 tttatgtggt gatatgatga taaaagttga ttatccattg aaaaatgctg cgaaagaaga
     7261 agagtgggtt gatgtaaaag ttgacagaaa taataaagtg gtcgatataa catggagatt
     7321 atctgttaag gatagcggta caaaaggcag taaccctgag ttatcgccaa taccttatag
     7381 cgatttagtt agtcttacta aaaatggcgt ggagttttac tggtcacgaa atggaagtcg
     7441 cggcggggga attggtggta atatcgttac agcgattggg gtttttaaag taaatgtaaa
     7501 ggctgagata aatattactc catctatgcg aacttttgat ttgatatcat cgttagatcc
     7561 agatatgcag tcgtcggtaa gtcttttggg gtttgaaaaa atataccata actatggtgg
     7621 ctattataat aacagcaagg atgaaataaa ggctct
//
LOCUS       DAPHCV010000167         7628 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.169, whole genome shotgun sequence.
ACCESSION   DAPHCV010000167 DAPHCV010000000
VERSION     DAPHCV010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.169"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            328..1038
                     /locus_tag="RH033_004279"
     CDS             328..1038
                     /locus_tag="RH033_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta-barrel
                     domain-containing protein"
                     /protein_id="HDV2993374.1"
                     /translation="MSTWVTITEAVEITTKAIKQKITPSDIYRHALSGNILLSVYFQS
                     PVILKKIQTFNGKIKFRQFVGDLLDKLCMLDRDGFIYGQNLRLCTEARYICPVQQIID
                     TPLLRKLNQFRTFVRNVRPGDELDVPAQVSEKNLTPPPGNSSGNLEQQIASTSQLIGS
                     LLAEDMNSEQAANIARGWASSQASGVMTDWLSRFGTARITLGVDEDFSLKNSRDGNPR
                     KWRFDATNSELCAERHEC"
     gene            965..3086
                     /locus_tag="RH033_004280"
                     /pseudo
     CDS             965..3086
                     /locus_tag="RH033_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(3256..4308)
                     /locus_tag="RH033_004281"
     CDS             complement(3256..4308)
                     /locus_tag="RH033_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDV2993375.1"
                     /translation="MFLVSLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNVLKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCKNASRMDQ
                     NLRAPVPMTWAFMMREAGFPAVRSIYELPLSDDVLDEVREEMRSLGSYIALNLEGSSQ
                     ERIFSFSIAENIIAKIQSETDIPIVIVYGPKGEDKARALVDCYNNVYRLSLSPSIKHS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVLESVIVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            4623..5939
                     /gene="shiA"
                     /locus_tag="RH033_004282"
     CDS             4623..5939
                     /gene="shiA"
                     /locus_tag="RH033_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HDV2993376.1"
                     /translation="MDSTLISTRPDERTVSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAMYLLAGCLISAITALLMKDSQRA"
     gene            6041..7495
                     /gene="amn"
                     /locus_tag="RH033_004283"
     CDS             6041..7495
                     /gene="amn"
                     /locus_tag="RH033_004283"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HDV2993377.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT         2047 a         1793 c         1738 g         2050 t
ORIGIN      
        1 agcctgaact atatttattc tgctgcaatc aatgcataca taacacaaat atcactcagg
       61 tacactactc aaaccacgct gggattttac ctcaagttat aatcatcacc ccgaaaatca
      121 ttcggcattt actcattaaa tagtcacccc ataggcctgt acatgtttac tcagaaatat
      181 acatcctttt ctctgtcata aaccctctga ttaatcataa ataaatactt gtgacaccaa
      241 tctttttcct taacggaacg aattgttgtg tagaaggaga taatattatg tcaaaaaaaa
      301 tatcagccat tgcttataac tcattatatg tcaacatggg tcactataac ggaagcagtt
      361 gagatcacta ccaaagccat aaaacaaaaa attactccta gcgatattta tcgtcatgcc
      421 ttgagtggca acatcctact atcggtctat ttccagtctc ctgtgatact taagaagata
      481 caaactttta atggaaaaat aaaattcagg cagtttgtgg gagacctact tgataaacta
      541 tgcatgcttg acagggacgg ttttatttat ggtcaaaatt taagactctg tacagaagct
      601 agatatattt gtcctgtgca acaaattata gatacccccc tgttacgcaa actcaaccag
      661 tttcgtacgt ttgttcgtaa tgtccgcccg ggtgatgaac tggatgtccc ggcacaagtt
      721 agtgaaaaaa atttaacccc gccgccgggt aacagcagtg gcaaccttga gcaacagata
      781 gccagtactt cacagctaat tggatctctg ctcgccgaag atatgaacag cgagcaagcg
      841 gcaaacatag cacgtggatg ggcctcttct caggcttcag gggtaatgac agactggtta
      901 agccgcttcg gtaccgcaag aatcacgctg ggcgtggatg aagatttcag cctgaagaac
      961 tcccgtgacg gcaaccctcg aaaatggcga ttcgatgcaa caaacagtga actatgtgcc
     1021 gaacgtcacg aatgctgaaa tcacgctggc agcctcgaag gacccggtga ttgccgacaa
     1081 taacgatctc acgacactaa cagcaccgtc gctgatacag agggcaatgc gatagccaac
     1141 actgaggtaa catttactct accggaagat gtgaaggcga acttcacgct gagcgatggc
     1201 gacaaagcgg ttactgatgc cgatggcaaa gcgaaagtca cgctgtaagg tacaaaagca
     1261 ggcgctcata ccgtgacagc atcgatggtt ggcggtaaga gtgagcagtt ggtggtgaac
     1321 tttactgcgg atacgctcac tgcgcaggtt aatcttaacg ttaccgagga caatttcatc
     1381 gccaataaca ttggaatgac caggctacag gcaacagtga ctgatggaaa cggcaaccca
     1441 gtagaaggta taaaagttaa tttccgtgga acctccgtca cgctaagcag caccagcgtt
     1501 gaaacggatg atcaggtttt cgctgaaatt cttgtgacaa gcaccgaggt cggactaaaa
     1561 acagtttcag cctctctggc agataaacct actgaagtca tctcgcgatt actgaatgcc
     1621 aaggtagatg ttaattctgc gacgattacc agtcaggaga tacctgaagg tcaggtcatg
     1681 gtcgcacaag acatagcagt taaagctcac gttaacgacc agtttggcaa cccggtgacg
     1741 catcaacccg cgacattcag tgcagcgcca tcctcgcaaa tgatcatcag ccagaatacg
     1801 gtctctacta atacgcaggg agtagccgag gtcaccatga cgcctgaaag aaacggctcg
     1861 tatactgtaa aagcatccct agcgaatgga gcttcacttg agaaacaact ggaggctatt
     1921 gatgaaaaac tgacactcac gtccagtccg cttatcggtg tcaatgctcc taaaggcgct
     1981 actctgacgg caatgctaac ctctgcaaat ggcactccag tggagggtca ggtcatcaac
     2041 tttagcgtaa cgctagaagg tgcgacgtta agtggcggaa aagtgagaac caactcttca
     2101 ggtcaggctc cagtcgttct gaccagcaat aaagtcggta catatacggt gactgcatcg
     2161 ttccataacg gcgtcacaat acagacacag acaaccgtga aggttactgg caacccaagc
     2221 accgcacatg ttgctagctt tatcgctgaa ccgtcgacta tcgccgccac caacagtgat
     2281 ttaagtacct taaaggcaac ggttgaggat ggcagtggta acctgatcga aggtctcact
     2341 gtgtacttcg ccttaaaaaa cggctccacc acattaacgt cattaacagc ggtgaccgat
     2401 caaaacggaa tcgcgacaac aagcgtgaaa ggagcgataa cggggagcgt cactgtaagc
     2461 acagtcacaa gcgcaggtgg aatgcaaaca gtagatatat cgctagtggc aggcccagca
     2521 gacgcctcgc agtccatcct taagaacaac cagtcatcac tgaaagggga ctttaccgat
     2581 agtgctgagc tacatcttgt tctgcacgat atatcaggca atccgatcaa agtttctgaa
     2641 gggatggaat ttgtgcaatc aggtacaaac gtgccctata tgaaaattag cgcaattgat
     2701 tacactcaaa atatcaacgg tgattacaaa gctactgtta caggaggcgg agagggtatc
     2761 gcaacgctga tccccgtatt gaatggtgtt catcaggcgg gcctgagtac cacgatagaa
     2821 ttcattagcg cggaaaccag accaatgacc ggtacagtgt cagtgaatgg tgcaaaccta
     2881 ccgacagctt cattcccttc gcagggattt accggggcgt attatcagtt gaataatgac
     2941 aactttgcac caggaaaaac ggcggctgat tattcgtttc aaactcggcc tcttgggtag
     3001 gcgttgatgc tactggtaaa gtgacattca aaaatgacgg tgatagcaat actgtaataa
     3061 tcacagcgcc cccaagatcc ggtggtgcta tttaccaaac agttccgcat gaatcgcgtt
     3121 cggtgtgact atgatcattt tcggtgagta ttgctttgat ccaaaggctg gttttctgtg
     3181 atgattgacc agcctcagag tattgatgac accaccgttc ataacagatc ggcattacta
     3241 tgcataagta ctgcctcaaa ttctcgccaa aacacttttt aatgcctttg cgaattcatc
     3301 cagacttata ttgtcaattt tttgcccaac gatgactgac tccaaaacat cggccattgc
     3361 gggccatcgc gttttgtaat cagcataaat tgcaacaaca ggggtattat aggcacttgc
     3421 catatgtaat attgaggtgt caggagttat tgcaatataa gcatctttta tgattgctgc
     3481 tgaatgttta atagagggtg ataaggataa acggtagaca ttattataac aatccactaa
     3541 tgccctggct ttatcttccc ctttgggtcc ataaacgatc actattggaa tatctgtttc
     3601 actttgaatt tttgctatta tattttctgc aatcgaaaat gaaaatatac gttcctgcga
     3661 gctaccttct aaattgagcg caatgtaaga tcctaacgat cgcatttcct cgcgtacctc
     3721 atcgagtaca tcatcgctta atggcaattc atatattgac ctgactgccg gaaaacccgc
     3781 ctcacgcatc ataaatgccc atgtcatggg tacaggagcc cggagattct gatccatccg
     3841 ggacgcgttc ttacacaaag gggaatagca cttcatggtt aaaccgacga cctggaaatt
     3901 cgtttttgct ttcaactgac tgataaatat catcgttttc aggttctttt tacgcatcgc
     3961 ctctatacaa agatccggcg taccgtattg ctgtgttatg ttcttcgcta aatcttttat
     4021 ttcttttaat gttgcgtgat cctgcatagt cattgtgact aatgttaatt tagtctgttc
     4081 aagtttgagc acattaaaga cttctaaatt aattgtcgac gtaacaatta atagatgctt
     4141 aattttatgc aattcaagcg cccgaataac aggaaagatg gccatagcat cgccaatctg
     4201 atcgggaata tggatgacaa caaagtctgt tttttcaata ttgaaattat aagctttata
     4261 atcgtagtag ctaaatgcaa tacgtctcaa caatgatact aaaaacataa ctaacctcgc
     4321 ctccctactg gttataatgc aatgcagtct atcagaatca tcagggtgcc attttgtgca
     4381 tatgcggact tttatgtttc atatctcgaa cctgtgggtc ctctgcttaa tccttaaaca
     4441 acaccagcaa ctcccgcgct ttcatcttcc agcgaatttt tcatgttgcc gctaatcagc
     4501 cataaaatca tttgcagatg cgctctgtcg aggtagtctc ataaggttcg tttatagatc
     4561 gacggcaatg tgagttacct tttccatact aattataaaa agacagtaca aacaggatca
     4621 ttatggactc cacgctcatc tccactcgtc ccgatgaaag gacggtttcg ttaagtcgcg
     4681 cccgacgagc tgcgttaggc agtttcgctg gtgccgtcgt cgactggtat gattttttac
     4741 tctatggcat caccgccgca ctggtgttta atcgcgagtt tttcccgcaa gtaagcccgg
     4801 cgatgggaac gctcgccgca tttgctacct ttggcgtcgg attccttttc cgtccgctcg
     4861 gcggtgtcat tttcggtcac tttggcgacc gactgggacg taagcgcatg ttaatgctga
     4921 ctgtctggat gatgggcatc gcgacagcct tgattggtat tcttccttca ttctcgacca
     4981 ttgggtggtg ggcacctatt ttactggtga cgctgcgtgc cattcaggga tttgccgtcg
     5041 gcggcgaatg gggaggcgcg gcgttgcttt ccgttgaaag tgcaccgaaa aataaaaaag
     5101 ccttttacag tagcggtgta caagttggct acggtgtagg tttactgctt tcaaccggac
     5161 tggtttcatt gatcagtatg atgacgactg acgaacagtt tttaagttgg ggctggcgta
     5221 ttcctttcct gtttagcatc gtactggtac tgggagcatt gtgggtgcgc aatggcatgg
     5281 aggagtccgc ggaattcgaa caacagcaac ataatcaagc tgccgcgaaa aaacgcatcc
     5341 cggttatcga agcgctgtta cgacatccag gtgctttcct gaagattatt gcgctacggc
     5401 tgtgcgagtt gctgacgatg tacatcgtta ccgcctttgc acttaattat tcaacccaga
     5461 atatggggtt accgcgcgaa cttttcctta atattggttt gctggtaggt ggattaagct
     5521 gcctgacaat tccctgtttt gcctggcttg ccgatcgttt tggtcgccgc agggtttata
     5581 tcacaggtgc gttaatcgga acgttgagcg catttccttt ctttatggcg cttgaagcac
     5641 aatctatttt ctggatagtt ttcttctcca taatgctggc aaacattgcg catgacatgg
     5701 tggtgtgtgt gcaacaaccg atgtttaccg aaatgtttgg tgccagttat cgctatagtg
     5761 gcgctggagt cggttatcag gttgccagtg tggttggcgg aggatttaca ccttttattg
     5821 ccgctgcact catcacttac tttgccggga actggcatag cgtcgccatg tatttgctgg
     5881 ctggatgtct gatttctgca ataaccgctt tgttgatgaa agacagtcaa cgcgcttgat
     5941 agcctggcga agatcatccg atcttcgcct tacacttttg tttcacattt ctgtgacata
     6001 ctatcggatg tgcggtaatt gtatggaaca ggagacacac atgaataata agggctccgg
     6061 tctgacccca gctcaggcac tggataaact cgacgcgctg tatgagcaat ctgtagtcgc
     6121 attacgcaac gccattggca actatattac aagtggcgaa ttacctgatg aaaacgcccg
     6181 caaacaaggt ctttttgtct atccatcact gaccgtaacc tgggacggta gcacaaccaa
     6241 tccccccaaa acgcgcgcat ttggtcgttt tactcacgca ggcagctaca ccaccacgat
     6301 tactcgtcct actctctttc gttcgtatct taatgaacaa cttacgttgc tgtatcagga
     6361 ttatggtgcg catatctcgg tgcaaccctc gcagcatgaa atcccttatc cttatgtcat
     6421 cgatggctct gaattgacac ttgatcgctc aatgagcgct gggttaactc gctatttccc
     6481 tacaacagaa ctggcgcaaa ttggcgatga aactgcagac ggcatttatc acccaactga
     6541 attctccccg ctatcgcatt ttgatgcgcg ccgcgtcgat ttttccctcg cgcggttgcg
     6601 ccattatacc ggtacgccag ttgaacattt tcagccgttc gtcttgttta ccaactacac
     6661 acgttatgtg gatgaattcg ttcgttgggg atgcagccag atcctcgatc ctgatagtcc
     6721 ctacattgcc ctttcttgtg ctggcgggaa ctggatcacc gccgaaaccg aagcgccaga
     6781 agaagccatt tccgaccttg catggaaaaa acaccagatg cccgcgtggc atttaattac
     6841 cgccgatggt cagggtatta ctctggtgaa tattggcgtg ggaccgtcaa atgctaaaac
     6901 catctgcgat catctggcag tactacgccc ggatgtctgg ttgatgattg gtcactgtgg
     6961 cggattacgt gaaagtcagg ccattggcga ttatgtactt gcacacgctt atttacgcga
     7021 tgaccacgtt cttgatgcgg ttctgccgcc cgatattcct attccgagca ttgctgaagt
     7081 gcaacgtgcg ctttatgacg ccaccaagct ggtgagcggc aggcccggtg aggaagttaa
     7141 acagcggcta cgtactggca ctgtagtaac cactgatgac aggaactggg aattacgtta
     7201 ctcagcttct gcacttcgtt ttaacttaag ccgggccgta gcaattgata tggaaagtgc
     7261 aaccattgcc gcgcaaggat atcgtttccg cgtgccatac gggacactac tgtgtgtttc
     7321 agataaaccg ttgcatggcg agattaaact tcccggccag gctaaccgtt tttatgaagg
     7381 tgctatttcc gaacacctgc aaattggcat tcgggcgatc gatttgctgc gcgcagaagg
     7441 cgaccgactg cattcacgta aattacgaac ctttaatgag ccgccgttcc gataataagt
     7501 tctgaacctt cttatcaaaa atagatgtga aggattaagt aagacatctg gcaaatagcc
     7561 cgcaataaac gcgggctgtt tgcttccttg ggcggatacg agttttatta tcgtcttaat
     7621 gatttcca
//
LOCUS       DAPHCV010000168         7448 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.170, whole genome shotgun sequence.
ACCESSION   DAPHCV010000168 DAPHCV010000000
VERSION     DAPHCV010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.170"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(120..2165)
                     /gene="dcp"
                     /locus_tag="RH033_004284"
     CDS             complement(120..2165)
                     /gene="dcp"
                     /locus_tag="RH033_004284"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HDV2993378.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQAPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDFAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAELRDRATREKLFTAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELVSIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDESQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGRRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNV"
     gene            2302..3048
                     /gene="ydfG"
                     /locus_tag="RH033_004285"
     CDS             2302..3048
                     /gene="ydfG"
                     /locus_tag="RH033_004285"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HDV2993379.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDSKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3137..3823
                     /gene="ydfH"
                     /locus_tag="RH033_004286"
     CDS             3137..3823
                     /gene="ydfH"
                     /locus_tag="RH033_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HDV2993380.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLEIFSALQKRDGDAVERAMTHHLQEISESVRQIR
                     QENSDWFSEE"
     gene            4000..4203
                     /gene="ydfZ"
                     /locus_tag="RH033_004287"
     CDS             4000..4203
                     /gene="ydfZ"
                     /locus_tag="RH033_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HDV2993381.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(4239..5699)
                     /locus_tag="RH033_004288"
     CDS             complement(4239..5699)
                     /locus_tag="RH033_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDV2993382.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAVMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVVADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEENVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYALMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(5788..7071)
                     /gene="ydfJ"
                     /locus_tag="RH033_004289"
                     /pseudo
     CDS             complement(5788..7071)
                     /gene="ydfJ"
                     /locus_tag="RH033_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
BASE COUNT         1948 a         1920 c         1711 g         1869 t
ORIGIN      
        1 gctggcgact gcgaaacggc attcgcatta gcggtgaagt ttgccctcac ccagctgctg
       61 caattactgc tataacgagg ctggtctggc gataaatcca ggccagccat tggtggtgtt
      121 tatacgttca agccacgatg ttgcagcatc ggcataatct gaggtgcctt accgcgccat
      181 tgtcgataca ggcgttccag atcttcgctg ttacctctgg aaagaatcgc ctcgcgaaaa
      241 cgccgcccat tttcacgcgt taatccgccc tgctcaacaa accactggta accatcatcg
      301 gccaacattt gcgtccacag ataagcgtaa taacctgcgg catatccgcc accaaaaata
      361 tgggcgaaat aactgctgcg atagcgtggc ggtatagcag gaagatccat attttccgcc
      421 accagcgccc gcaattcaaa atcatcgaca tcctgcattg cttcgttttc ttccaggcaa
      481 tgccagcgca tatcgagaag tgcggcgcta agcagttcgc tcatctcata ccctttattg
      541 aacaggctgg cattacgcat tttctgttgc agttcatcag gcattgctgc cccgctctga
      601 taatgccggg cgtagcgagc gaatacctgc ggatgcgttg cccagtgttc gttgatttgc
      661 gacggaaatt cgacaaaatc acgcggcgtg ttggtgccgg aaagcgtggc ataacgctgg
      721 cgggcaaaaa ggccgtgcag cgtatgacca aattcatgga ataaggttat gacatcatcc
      781 cagagtaaca acgcaggctc cccagcggcg ggtttctgat aattgcagac gttataaatt
      841 accggatgcg tttcattcag cgttgattgc tcaacaaaat tgcccatcca tgcaccgccg
      901 ctttttgaat cacgggcgaa gaaatcaccg taaaataacg ccagccccac gccattatga
      961 tcaaaaattt cccacacacg aacgtcagga tggtagacag gaatatcaaa acgttcgaca
     1021 aacttaatac cgaagagctg attcgcggtc cagaatacac cttcattcaa caccgtgttt
     1081 aattcaaaat atggcttgag ctgcgattca tcgagatcga atttctcacg tcttacctgc
     1141 tcggcataaa atgcccagtc ccacggctgc gcgctaaacc cgccctgctg cttatcaata
     1201 accgcctgta tggagactaa ttcatcgctc gcacgttgac gcgccgctgg aacaatttcc
     1261 cgcataaagt tgagtgctgc ttctggcgtt tttgccatct gatcggcgat tttccatgcg
     1321 gcataatgag ggaaaccaag cagtttcgcc tgctgcgcgc ggatctccac cagacgttga
     1381 atgatagcgc gggtatcatt ggcatcattt ttttccgctc gcgtccagcc cgcagtgaac
     1441 agtttttcac gcgtcgcgcg atcgcgcagt tcagcaagcg ctggttgctg ggtggtattc
     1501 agcaacggaa tcagccattt gttatccaga cctttctcgc gagccgcctc tgccgccagc
     1561 gcaatctctt gctcactcat tcctgccagc tgcgcgaaat cgttcacaac cagaccgccg
     1621 gatttatttg ctgccagtaa ccgctggtta aactggctgg tcagggtcgc agcttctgta
     1681 ttcagtactt ttaattttgc tttatcagct tgtgcaagtt tggctccggc aaggacaaaa
     1741 cgttgatgaa tgacctccac caggcggatg gattcactat caagtcccag ggattcacgg
     1801 cgttgccaga cagcatctac ccgcgcgaat aattcaccgt tcagatagat atcattagcc
     1861 agttccgcca gttcagcgga aaactgttca tcaagacgct gtaattcatc attagtatgc
     1921 gccgcagtca tcgcaaaaaa gacgctggta acgcgggtaa gtaattctcc gctttgttcc
     1981 agtgccagaa tagtattgtt gaaatcaggc gcttgcgggt taagcgcgat ggcagcaatt
     2041 tcagcccgct tttgctgcat tccctcatcg aatgccgggc gatagtgatg attggcaatt
     2101 tgatcaaaat ggggagccag atacggcagt gtgctttgca caaggaaagg attcattgtt
     2161 gtcattttct tctcctgaac gcgaggtgtt ccatagcgta ggcttactga taacggagtg
     2221 caatcttgca aaccagtatt acccgctctt aagcatcacg tgctatgtta ttgacacaca
     2281 aaagcgttga ggaacagtga gatgatcgtt ttagtaactg gagcaacggc aggttttggt
     2341 gaatgcatta ctcgtcgttt tattcaacaa gggcataaag ttatcgccac tggccgtcgc
     2401 caggagcggt tgcaggagtt aaaagacgaa ctgggagata atctgtatat cgcccaactg
     2461 gacgttcgca accgcgccgc tattgaagag atgctggcat cgcttcctgc cgagtggtgc
     2521 aatattgata tcctggtaaa taatgccggc ctggcgttgg gcatggagcc tgcgcataaa
     2581 gccagcgttg aagactggga aacgatgatt gataccaaca acaaaggcct ggtatatatg
     2641 acgcgcgccg tcttaccggg tatggttgaa cgtaatcatg gtcatattat taacattggc
     2701 tcaacggcag gcagctggcc gtatgccggt ggtaacgttt acggtgcgac gaaagcgttt
     2761 gttcgtcagt ttagcctgaa tctgcgtacg gacctgcatg gtacggcggt gcgcgtcacc
     2821 gacatcgaac cgggtctggt gggtggcacc gagttttcca atgtccgctt taaaggcgat
     2881 gacagtaaag cggaaaaaac ctatcaaaat accgttgcat tgacgccgga agatgtcagc
     2941 gaagccgtct ggtgggtgtc aacgctgcct gctcacgtca atatcaatac cctggaaatg
     3001 atgccggtta cccaaagcta tgccggactg aatgtccacc gtcagtaatt tttatacccg
     3061 gcgtaactgc cgggttattg cttgtcacaa aaaagtggta gactcatgca gttaactcac
     3121 tcacaagcaa gaacgaatga ccgtcgaaac gcaacttaat cccacacagc ctgtcaatca
     3181 gcagatttat cgtattcttc gtcgcgacat tgtccattgc ctgattgcac caggcacacc
     3241 gttgtcggaa aaagaagttt ctgttcgttt caatgtgtca cgccagccgg ttcgtgaagc
     3301 ctttattaaa ctggcggaaa acggcctgat tcaaattcgt ccgcagcgtg gcagctacgt
     3361 caacaaaatt tccatggccc aggtgcgcaa cggcagtttt atccgtcagg ccattgagtg
     3421 cgcggtggcg cgtcgggcgg cgagcatgat taccgaaagc cagtgctacc aactggaaca
     3481 aaatcttcac cagcaacgca ttgccataga gcgcaagcaa ctggatgatt tttttgaact
     3541 tgatgacaac ttccatcaac tcctgacgca gattgccgac tgtcaactgg cgtgggatac
     3601 cattgagaac ctgaaagcga ccgttgatcg cgtgcgctat atgagtttcg accacgtttc
     3661 tccaccagaa atgctgttac gccagcatct tgagattttc tctgccctgc aaaaacgtga
     3721 tggcgatgcg gtagaacgtg caatgacgca ccatttgcag gaaatcagcg aatccgtgcg
     3781 ccagatccgt caggaaaaca gcgactggtt tagcgaagag taattcattt cctctcatcc
     3841 catccggggt gagagttttt ccctccgtca tatggctcat gcatgcatca aaaaagatgt
     3901 gagcttgatc aaaaacaaaa aatatttcac tcgacaggag tatttatagt gcgcccgtta
     3961 cgtgggcttc gactgtaaat cagaaaggag aaaacaccta tgacgaccta cgatcgtaac
     4021 cgtaacgcaa tcaccactgg cagccgtgtt atggttagcg gcaccggtca cactggcaag
     4081 atcctgtcga ttgatactga aggtctgacc gctgagcaaa tccgccgcgg aaaaaccgta
     4141 gttgttgaag gttgtgaaga gaaactggca cctctggacc tgattcgtct cggcatgaac
     4201 taagcgtgtg aatgccgccg atggcggcat tgcttttttt acttcacgga atatttcgcc
     4261 acggtcgctt tcgcaccatg cgctaataaa gacaagtacg cttccgtcac ttttgcagta
     4321 aacaagctat tgtctggcaa atcatcacca aagatcgcct taatcgccag caatgactgg
     4381 acgcgcgctt tcccttcggc actactttgt acagccttct gaataacagg taacagtggg
     4441 tcactgattt ctatcggatt tccctgttca tcaacaccac cgacataacg catccaacct
     4501 gcgacgccca gcgccagcag atcgaacttg ctgtcatgcg ccagatgcca gcgaacagaa
     4561 tccaacatcc gctgtggcaa tttctggcta ccatccatcg caatctgcca ggttcgatga
     4621 cgtagcgccg ggttgctata gcgttcaatt aatcggttag cgtaatcttg caaatcaacg
     4681 ccctgcactt tcaacgtcgg cgcttgttcc tgcaacatca aggcatacgc tgcatgacga
     4741 taatgttcat cttccatgca gtcattaatg tgctgatatc cggcaagata ccccagatac
     4801 gccaggaatg aatgactgcc gttgagcatg cgcaacttca tctcttcata aggcagcaca
     4861 tcactaacca gttcggctcc cgctttttcc cattccggac gtccggcaac aaagttatct
     4921 tctattaccc actggcggaa aggttcacag gcaacgcccg caggatcgcg cacaccggta
     4981 agttgttcga ttttcgccag cgtatcctcc gtcactgcgg gcacaatacg gtccaccatt
     5041 gttgatggga aagtcacgtt ttcttcgatc cattgtgcca gttttacatc aacagcttgc
     5101 gcgtaggaag tgacaacgtc acgcataaca tgaccgtttt ctggcatgtt gtcacatgac
     5161 atgacggtaa atgcgggaag tcctgccgct ttacggcgag ccagcgcctc gacaatcacc
     5221 cctgttgcag ttttcggctg gtggggattt tgtacgtcgg caacgaccat cgggtgatcg
     5281 agcattaact gtccggtcgc cggagagtgg aaataccctt tttcggtgat tgtcagagag
     5341 acaatcgcga tttgcggttc acacatcaca gccaacacgg tttctaagcc atcaatctgt
     5401 acgtgcaagg cttttttaac gacgccaacg acgcgagccg tccacgcatc ggccgacatt
     5461 tccgcaacgg tataaagatt atcttgctgt tgtaaatcgg caatttgctg ttcgccgccg
     5521 attaaattga cctcataata tccccagtca ctgaaatgtt ccgtagcaag aatatcggca
     5581 tacacaccct gatgcgcacg gtgaaatgca ccaaagccta aatgaacaat tcttggggcc
     5641 aggttattac gatcataaac agggagtgtc gcttttgctg ataacaaatt atttcccata
     5701 acaattcctt aaatataaat atggcaagct acatgttttg ttatatgaat aaaaatcccc
     5761 tctccggtaa gagaagggat tgaggattta aagacttctg gaaggttgcg cagctcttac
     5821 aacacgcggt tgatcttccg cagcgtcttc cagcgcactt aaatcacggt ctttcacttc
     5881 tggcattttc agcgcagaga ttaaaccaat cactgaatat gccatgatca taatggcgat
     5941 cggataccag gattccgtca tggtgcagaa aatacccgcc aggataggac caaaaccgga
     6001 agcgataaga ccaccaattt cttttgaaat agccatccgg gtaaagcggt ttttacagcc
     6061 gaacatttct gccatggtaa tgttttccag agcaaataat cccagcaccg cacagttatg
     6121 aatcacaatc agtgcaacca taatggtgct cggggcatag cttttatcta cgatgataga
     6181 aagcattggc cacgccagca caatcgcaga ggtattcata ataatatacg ggatccggcg
     6241 accaatttta tcggataacc aaccaaggaa cggaatggtc ataaagccga gaatcgaact
     6301 gatcatcaat gcatctgttg gaattgcttt gttaaacaat aacgtctgta ctaaatagcc
     6361 tgcaaggaaa gtctgaatta acccggagtt acctgcctga ccaaaacgca gtcctgttgc
     6421 cagccagaag gatttgctct ggaacatgct accagcaggt gcaggttttg ctgtcggttg
     6481 gttgctgtcg ttaaccttct caaagaccgg gctttctttc agattcatac gcaaccagat
     6541 agcaaagacc atcacgacaa cgctcgccag gaacggtata cgccatcccc acgccagcag
     6601 ttcctcttta ctgagaatga agaacataaa ggcccagatt gccgttgcgc ttaaggttcc
     6661 gcagttagtt cccatagcca caaatgagga gataattccg cgcttacctt ttggcgcata
     6721 ttccgccagc atcgtaccgg caccggaaat ttccgcacct gcacccaatc cctgaataat
     6781 acgcagcgtc accagcaaga tgggtgcaaa aacaccaatc tgtgcatagg tcggtaacac
     6841 accaattaag gtggtacaga tccccatcat ggtgatggta ataaagagca cttttttacg
     6901 ccctattctg tcgcccattt tgccgaaaat aaatgctccg acaatacgcg ccacataacc
     6961 tgcaccgtag gttcccattg ccagaattaa cgccattgcc gttgatgatt caggaaaaaa
     7021 tatttcatga aacactaacg ctgcaccgag cgaatataac tggaaatcca taattcacag
     7081 gtgttttttc ccatcctgtg gtttccttgg cgttttctag gttttttcag atagttgcat
     7141 tttttaaaaa gcatcctaag ttcgatctca gtgtctatct ggggcctatt cctgtcccat
     7201 atatgcccca aaaaaaactc cccaacagat aagtagtttt ttcatggatt tacgcgtaaa
     7261 atcaataacg gctggaaatc attcaatact cgcactatcg aaaaatttac cagccaaccg
     7321 cagcacgttc ttgcataagg cgtgtctgcg gtttttcaac tattcagata catcactccc
     7381 atcacattca ttcctccgca tcaaaggcat ataggctata tcaccttgat atttttcttc
     7441 ttcagata
//
LOCUS       DAPHCV010000169         7440 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.171, whole genome shotgun sequence.
ACCESSION   DAPHCV010000169 DAPHCV010000000
VERSION     DAPHCV010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.171"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(531..914)
                     /locus_tag="RH033_004290"
     CDS             complement(531..914)
                     /locus_tag="RH033_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HDV2993383.1"
                     /translation="MQNDYNDLKPIAEMMYPNPAVEELKAIADKMCLSERLVDMNQVM
                     EITTLSRRTLLNLEASGEFPERVQVTEGRKAWYLSEVIDWINNIPRASEYCRVPVPKK
                     PDAALCLKIERVRRNARDGRYKLIG"
     gene            complement(926..1267)
                     /locus_tag="RH033_004291"
     CDS             complement(926..1267)
                     /locus_tag="RH033_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HDV2993384.1"
                     /translation="MATHYTELMAGTEALVTTLGIFSANKGVIPAFTPLMQEDATGAL
                     VVWDGSSVGKAVYVSAVQIDTAKKTQAQVYKTGVLNVDALNWPESVKELSVKVAAFVG
                     SGISVQPLARV"
     gene            complement(1277..2317)
                     /locus_tag="RH033_004292"
     CDS             complement(1277..2317)
                     /locus_tag="RH033_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HDV2993385.1"
                     /translation="MVDLYSPTQLVQVVNAVDVQKQLNALFTSLFFTRSVMFESRDII
                     LDTIDDPNIPIAAFCSPMVGSKVSRDEGYESKTIRPGYMKPKSSIDPNKLAVRPAGVS
                     PEQYNAFGARNIKVKQAIVNQAKAIRARIEWLAVQAITTGKNIIEGDGIERYELDWNI
                     KSQNIITQSGGTEWSGKDKETFDPNDDIESYAEFSEGVTNIIIMGGNVWKKYRSFRAI
                     KEALDTRRGSNSELETALKDLGDSVSFKGYMGDVAIVVYSGRYTDEDGTEKHFLDPDL
                     MVLGNTALQGIVAYGGIQDPELIRMGLTKAELAPKNYIVPGDPAIEYVQTHSAPQPIP
                     ARINRFVTVRIG"
     gene            complement(2534..2983)
                     /locus_tag="RH033_004293"
     CDS             complement(2534..2983)
                     /locus_tag="RH033_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDV2993386.1"
                     /translation="MTAQISVYGRLVDDPQTKQTSKGTPMTLARMAVSLPCSQSDDGL
                     ATLWLSVLAFGRQADALAKHHKGELLSVAGNMQMSQWTGQNGETRQGWQVIADSVISA
                     RSVRPGGKKGQQGQATDALNRAKQQADQQGSQPPEGDNEQWGDDIPF"
     gene            complement(2980..3237)
                     /locus_tag="RH033_004294"
     CDS             complement(2980..3237)
                     /locus_tag="RH033_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993387.1"
                     /translation="MHTSGKLNKHIKPHYRALDMAEHWLRVAIKAIDRNAGEGYAKAH
                     PELISAFMATAAANFATLTEREIAEAEEVTTINIKSGEQAA"
     gene            complement(3527..5278)
                     /locus_tag="RH033_004295"
     CDS             complement(3527..5278)
                     /locus_tag="RH033_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDV2993388.1"
                     /translation="MKLAPNLKKQPRDRLTEVIIFAGSDAWSHAKEWREWAGKHIAAD
                     DVPPVVLADEQLKNITDYRIIDEDRQCVRVYRAGHITEHSMTQIVTLLAVAGVKTVHE
                     YAGITDTSPVDLSEQLPRLKEECERGESLVLNLPTKQKAQLSQMADSERAQLLADRFD
                     GVCVHAESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIA
                     DPMGEPSGDLLPFTNGVLNLKTGEFSPHSPEHWSTTHNGIEYTPPVAGENIRDNAPNF
                     HKWLEHAARKDPRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIATLLAG
                     KQNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYE
                     KRFTTIIRAVVLATNNDPMIFTERAGGVSRRRVIFRFDNIVREDEKDKELPEKIAAEI
                     PVIIRRLLANFADPEKARALLLEQRDGDEALAIKQQTDPVVELCAALEFLEEARGLMM
                     GGGGDTVKYTTRNSLYRVYMAFMAYTGKGKCLSVNEFGKAMRSAAKVYGYEYITRKVK
                     GVTQTNATTTDDCDAFL"
     gene            complement(5275..5574)
                     /locus_tag="RH033_004296"
     CDS             complement(5275..5574)
                     /locus_tag="RH033_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HDV2993389.1"
                     /translation="MEMKNSGFIASGPARPEFMNGDIYRDKYGGTVTIKGVAERRITY
                     RREGYSYDCVMPVYQFRRDFSLVYAAPRSKPISREKARGNIQKMKSMINAFRGKK"
     gene            complement(5592..5813)
                     /locus_tag="RH033_004297"
     CDS             complement(5592..5813)
                     /locus_tag="RH033_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993390.1"
                     /translation="MNTEREVFFKLLACAERSLTLNNSAKAILNMWLDCINDNEDANI
                     AYGLLSLIDESAEKLNDAINSALLSNKSS"
     gene            complement(5814..6005)
                     /locus_tag="RH033_004298"
     CDS             complement(5814..6005)
                     /locus_tag="RH033_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993391.1"
                     /translation="MQEITLHEAAERAHQTEIICRLLEVYPNKITDADISALASLLAR
                     LSGSVASFLIEEESKLVGD"
     gene            complement(6005..6190)
                     /locus_tag="RH033_004299"
     CDS             complement(6005..6190)
                     /locus_tag="RH033_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993392.1"
                     /translation="MLKTFRVFARAVNPLGHTIGITQNVKAVNVQTAIAAVRSESSEY
                     GLSQVIISAVYELKEVH"
     gene            complement(6183..6548)
                     /locus_tag="RH033_004300"
     CDS             complement(6183..6548)
                     /locus_tag="RH033_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001588497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HDV2993393.1"
                     /translation="MAGTQHTQTHPKFIYTFLALHHDRMADGATTVHVAADTLVDARK
                     MVKEMGYTAAFWKGREENTLFIQKCENNFIWRFIALSTAQPRMITIEAISEQEARQQS
                     PTGCVMVFAARIRQEVCRA"
     gene            6757..7059
                     /locus_tag="RH033_004301"
     CDS             6757..7059
                     /locus_tag="RH033_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993394.1"
                     /translation="MSVYKNAKSQMTTIRVPHDVMEGMESVKLDGESNAGFIVTAMRG
                     EIARRQAEGSGENPLVSSLDALAKVEQIGIKAAEEIGQLVAVAREELQRRKAKESE"
BASE COUNT         1891 a         1902 c         1548 g         2099 t
ORIGIN      
        1 taaagattta tttttaaaaa gcctgcctaa aatttcttca agataaacaa taggcgtgat
       61 cgattcttat cacgcctaat tttcatccct caataattca ttgaaggagc aacaaaattt
      121 aaagtcaacc ataacagaat atatcagtaa cttgttacat tatctgttat agcagaacct
      181 cacatcattc gccaaaactg caagcaacta gatgtcttgc tactttaaat cctgaacagt
      241 acgcatagtg acacaataac ctctataagc atttggggtg gcctcgatgc ttacttttag
      301 ataaaaatga gaaaggaaat gacggagacc caaaagcctg ttttcagcac ctgtcatttc
      361 ctttcttttt agagggtatt tctagtaaaa acactaagtt acgaggaaga agaacggaaa
      421 cggcaaatcg cagaaaattg tcataaatag caagaatctg cgcgcctgac gccccgtaac
      481 gttctggatc accggaaagg acccgccagc cagagcgggc cctaatttca tcaaccaatc
      541 agcttatagc gaccatcccg tgcattgcgg cgtacacgct caatcttgag gcatagcgcc
      601 gcatctggct tttttgggac aggtacgcgg caatattcag aagcgcgagg aatattattt
      661 atccagtcga tcacttcact taaataccag gccttacgcc cttccgtaac ctgcacacgc
      721 tccgggaact ctccactagc ctcaaggttt agcagtgtac gacgactcag ggttgtaatt
      781 tccatcacct gattcatatc aacaaggcgc tcgcttaaac acattttgtc agcgatagct
      841 tttaattcct ctacagctgg attcgggtac atcatttcgg caattggctt aaggtcattg
      901 taatcattct gcattgtatc cccctttaca cacgagccag cggctgaaca gaaatacctg
      961 agccaacaaa cgctgcaacc tttactgaca gttctttaac agactcaggc cagttcagag
     1021 catcaacatt taagacacct gtcttataga cctgagcctg tgtttttttc gcggtgtcga
     1081 tttgtacagc ggaaacataa accgctttac ctacgctcga accatcccac accaccagtg
     1141 cacctgttgc atcttcctgc atcagtggcg taaatgcagg aattacccct ttattagctg
     1201 aaaatatccc cagcgtagtc accagtgctt cagtgccagc catgagttca gtgtaatgag
     1261 tagccattgc tcccccttag ccaatgcgaa cggtaacaaa acgattgatg cgggccggta
     1321 ttggctgtgg tgctgaatgt gtctgcacat attcaatagc cggatcacca ggcacaatat
     1381 agtttttcgg tgcaagttcg gctttagtca gccccattcg gattagctcc ggatcctgaa
     1441 taccgccata ggcgacaatc ccctgaagag ccgtattgcc aagcaccatc aaatcaggat
     1501 caaggaaatg tttttctgtt ccgtcctcgt cggtataacg cccgctgtaa acaacaatcg
     1561 caacatcgcc catataccct ttaaaactca ccgaatcacc aaggtcttta agggccgttt
     1621 ccagttcgga attagaacca cgacgggtat ccaaagcctc ttttatcgct ctgaatgaac
     1681 ggtatttctt ccatacatta ccacccataa tgatgatatt agtgacgccc tcactaaatt
     1741 ctgcgtagct ctcaatatca tcatttggat caaaagtttc tttatcctta cctgaccact
     1801 cagtaccgcc agactgagtg atgatatttt gtgattttat attccagtcc agctcataac
     1861 gttcaatacc atcgccctca atgatatttt tccccgttgt gattgcctga acagcaagcc
     1921 attcaatacg tgcacgaata gctttagcct gatttacaat cgcctgttta actttaatat
     1981 tacgcgcccc aaaagcattg tattgctcag gtgacacacc agcagggcgc acagctaact
     2041 tatttggatc aatgctgctt ttcggcttca tatagcctgg acgaattgtt tttgattcgt
     2101 acccttcgtc acgtgaaact ttactaccca ccataggaga acaaaacgct gcaattggga
     2161 tatttggatc gtcgattgta tcaagaataa tatcgcgcga ttcaaacatt accgagcgag
     2221 taaaaaacaa actggtaaac aacgcattta gttgtttttg tacatctaca gcattaacta
     2281 cctgtacaag ctgagtaggc gaatataaat caaccatacg catcctcttt gcattcatta
     2341 aaaataattg tggatatatg ctatcaccga tatttgtcat gcgaacacat gcaaccgagt
     2401 gcaatgttgt ataaagtttt ggggtgacaa cttcagtgcg gacaattagt gttaatatct
     2461 tcactccctt tggtcgggat ttatgtagca tgccggaaaa tttatttttt ccggcttttt
     2521 ttattggcaa tatttaaaac gggatatcat ctccccattg ctcattatct ccctctggtg
     2581 gctggcttcc ttgctgatct gcctgttgtt ttgctctgtt cagtgcgtca gtagcctgcc
     2641 cctgttggcc ttttttgccg cccggtcgca ccgatcgcgc actgattacg ctgtctgcga
     2701 taacctgcca gccctgccgc gtttcgccgt tctgtccagt ccactggctc atctgcatgt
     2761 tacccgccac gctcaggagt tcgcctttgt gatgctttgc cagcgcgtcg gcttgtctgc
     2821 caaacgccag gacagataac cacaacgtcg ccagaccgtc atccgactga ctgcagggca
     2881 gtgataccgc catacgcgcc agcgtcatgg gggtgccctt gctggtctgt tttgtctgcg
     2941 ggtcgtccac caaccgcccg taaactgata tttgcgccgt catgctgcct gctctccgga
     3001 cttaatattg attgttgtca cttcctccgc ttcagcaatc tcccgttcgg tcagcgtggc
     3061 aaagtttgca gccgccgttg ccatgaatgc gcttatcagt tcgggatgtg ctttcgcgta
     3121 tccttccccg gcgttgcggt ctattgcctt aatcgccacc cttagccagt gttcagccat
     3181 atcaagggcg cggtaatgtg gctttatatg tttgttcagt tttcctgatg tgtgcatttt
     3241 tatttttacc ccctcgttta aaaagttttt tgtgcaccac caccttgtct accttgtcta
     3301 cctgattagt tatcaggcca gtaatggcgc gggtttcagg gaggtagaca gccccaaata
     3361 gctgtctacc tcatctctac ccgtctcctt acctgtctac aaaaatgggt agataaggta
     3421 gataacaggt agacagtgaa aaatagttat ctacctgcat taatacattg aaataaaagt
     3481 attttctttc agtcaggtag acaaggtaga taaccattgc cattttttat aaaaacgcat
     3541 cgcaatcatc agtagtcgtt gcgttggtct gcgtgactcc cttaactttt cgcgtaatat
     3601 attcatatcc gtaaactttc gccgctgacc tcatagcctt tccgaactca ttcacgctca
     3661 aacatttccc ctttcctgtg tatgccatga aggccatata gacacggtaa aggctgtttc
     3721 tggtcgtgta cttcacggtg tcaccaccac cacccatcat tagcccacga gcttcctcca
     3781 gaaactccag cgccgcgcaa agctcaacaa ccggatccgt ttgctgcttt attgccagag
     3841 cttcatcacc gtcacgctgt tccagtaata aagcccgtgc cttttcaggg tcagcaaaat
     3901 tagccagcaa gcggcggata attacgggga tttctgccgc tatcttttcc ggtaattcct
     3961 tgtctttttc gtcctccctt acaatgttgt cgaaccggaa aatcacccga cggcgtgaca
     4021 cacctccggc ccgttcggta aagatcatcg ggtcgttatt ggttgccagt accaccgccc
     4081 ttattatcgt cgtgaatcgc ttctcatatt tcgggttaat ttcaacggga tcgcctcccg
     4141 tgattttctt gatgcccgtg ccttcccccg tatatttcgg ctgatcggca aggacgataa
     4201 gacgactccc gacaacctgc gcgcgccctc ctgcatcatc gagtgatgtc atctccgcgc
     4261 ttacggtgtt ctgtttgcca gcaagcaggg tggcaatatg ggtaaatgta ctcttaccgc
     4321 ttcccccgtc tccggtggcc tcaatgaaca tctgccagtc gtagcggttc gccataatca
     4381 tgtataacgc ggcacatata cgcatcatct tacgcgggtc ttttcttgca gcatgttcaa
     4441 gccatttatg gaaatttggc gcattatcgc ggatgttttc ccctgctact ggtggcgtgt
     4501 actcaatgcc attgtgcgtg gtgctccagt gctccggcga gtgcggagaa aattcccccg
     4561 ttttcaggtt aagcacacca ttagtgaacg gtagcaaatc accggacggc tcccccatgg
     4621 ggtcggcaat aacttttaac gcttccacgg cgttattgat tacacgtttg ctgaacgtgg
     4681 ccctgtgctc tgaatagatc gccaccattt cgcggctcag ctccattgtg ctgaccggac
     4741 accatacccc gccgcgccat acgtggacga tttcactttc tgcatgaaca cacacaccat
     4801 caaagcgatc ggcaagtagt tgtgcacgtt cactgtctgc catctgtgaa agttgcgcct
     4861 tttgcttcgt cggaagatta agcaccagac tttccccacg ctcgcattcc tctttgagtc
     4921 gcggcaactg ctcggataaa tccactgggc tggtgtcagt aatccccgcg tattcgtgta
     4981 cggtcttcac tccagccaca gccagtaacg taacaatctg cgtcatgctg tgctctgtga
     5041 tatgtcctgc gcggtaaaca cgcacacact gacgatcttc atcaatgatc cggtaatcgg
     5101 tgatattttt cagttgctca tcagccagca cgacaggcgg cacatcgtcg gcggcaatat
     5161 gtttacccgc ccattcccgc cactctttcg catggctcca cgcatcacta cctgcaaaga
     5221 tgattacctc tgtcagtctg tcgcgtggct gtttttttaa gttcggtgcc agtttcattt
     5281 tttgcccctg aatgcgttaa tcatgctttt cattttctgg atgtttcccc gcgctttttc
     5341 cctgctgatg ggcttactgc ggggtgcggc atataccagg gaaaaatcac gccggaactg
     5401 ataaacaggc atcacgcagt catagctata cccctcacgg cggtaagtga tgcgccgttc
     5461 tgccacgcct ttaatcgtta ccgtgccgcc gtatttatcg cggtaaatat cgccgttcat
     5521 aaattcaggc cgagcggggc cgctggcaat aaagccagaa tttttcattt ccatattatt
     5581 tattcctcga cttaactcga cttatttgat agcagggcac tatttattgc gtcattgagt
     5641 ttttctgctg attcatcaat aagtgacaac aggccataag caatatttgc atcttcattg
     5701 tcatttatgc aatcaagcca catatttaat attgcttttg ctgaattatt taaagttaat
     5761 gatctttctg cacatgctaa caatttaaaa aagacttccc gttctgtatt catttaatcc
     5821 cccaccagct tactttcttc ctcaatcagg aaactagcga cacttcccga aagacgcgcc
     5881 agtaggctcg ccagcgcgga tatatcagca tctgtaattt tgttcgggta tacctcaaga
     5941 aggcggcaaa taatttctgt ctggtgcgca cgttcagcgg cttcgtgtaa tgtgatttcc
     6001 tgcattaatg cacctctttt aattcataca ctgctgaaat aatgacttgt gataagccat
     6061 attctgatga ttcgcttctc accgcagcaa tagccgtctg aacattaaca gccttcacgt
     6121 tctgagtgat accaattgtg tggcccagtg ggttaacagc tcgggcaaat acacggaagg
     6181 ttttaagcac ggcacacctc ctgacgaata cgggcggcga ataccatcac gcagccagtt
     6241 ggggattgct ggcgggcttc ctgttcgctg atggcctcga tggtaatcat gcgcggttgt
     6301 gccgtactca gggcgataaa acgccagatg aaattgtttt cgcatttctg aataaacagc
     6361 gtgttttctt cacgcccttt ccagaaagcc gcagtatagc ccatctcctt aaccatcttg
     6421 cgggcatcaa ccagcgtgtc agcggctaca tgtaccgtag ttgctccatc tgccatgcgg
     6481 tcgtggtgta gtgccagaaa ggtgtatata aatttagggt gagtttgggt atgctgtgtt
     6541 ccagccataa tcgttacctc gtttaacggt ttggttagaa gcccggttag tgttcgcgca
     6601 ctgccgggtt tcgtcgtttt tatgaatcga tcattgtgag atacatagcg accataatgt
     6661 gagatataca ttatattgtg gtgatataca ttgcaagtgt ttttatatct cacttttgtg
     6721 tatagtgata tacacataac aaattggtga ttaactatgt ctgtatacaa aaatgcaaaa
     6781 tcgcaaatga caacgatcag ggttccccac gatgttatgg agggcatgga atccgtaaaa
     6841 ctggacggcg agagcaacgc cggattcata gtaaccgcca tgcgcggtga gatcgcccgc
     6901 cgccaggcag aaggaagcgg agaaaatccc ctcgtgtctt cactggatgc cttagctaag
     6961 gtcgaacaaa tcggcatcaa ggcagcggag gaaatcgggc aactcgtagc cgtcgctcgt
     7021 gaagaactcc agcggcgtaa agccaaagaa tctgaataat tagtatcagc gccgtgatgt
     7081 gagtaactac ggcgcattgc tatgtaaata ctggcaataa acagaaaagg tagttctact
     7141 ccgaataatt ttatctgaca ctactcctga actaacatgc gcttatctta caggatataa
     7201 atataaatcc ataaaatcac gattaaataa agtcgctcca aacataaacc acacccaacg
     7261 cttaacaaga tagcaacaaa cagataaata acttgcagaa atatttatcg caaggattat
     7321 cattattaat gacaaatcac tttaccaagt cattcccctc tcttatcata aagagaaagt
     7381 aataaataag ttaagggagt tagaatgcta tgaatctaaa aaaaatagcc acaaacacaa
//
LOCUS       DAPHCV010000170         7176 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.172, whole genome shotgun sequence.
ACCESSION   DAPHCV010000170 DAPHCV010000000
VERSION     DAPHCV010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.172"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..569
                     /locus_tag="RH033_004302"
     CDS             <1..569
                     /locus_tag="RH033_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDV2993395.1"
                     /translation="KHFMRNEHKAVEEVIDSPLSEVANLIKVLQSCQGKVVFIGVGKS
                     GIIARKLAATFASTGTPSFFVHGTEAVHGDLGMVAKDDVVILISNSGETTEILATLPS
                     LKKMGNYLISFTRSHRSSLAISCDLSVEIPVKSEADNLGLAPSCSSTVVLVVGDAVAL
                     ALSELKKFTRADFGLYHPGGALGIKANS"
     gene            640..1587
                     /locus_tag="RH033_004303"
     CDS             640..1587
                     /locus_tag="RH033_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDV2993396.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENHFNLIIKNNVEHLVKSEST
                     VLRESICAVIAGKDCYQADTLSRLPGVKIISRFGTGIDNIDLHTARQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGCELQGKRIGLLGYGNIGKTLA
                     KRLSGFDVELLAFDKQPDFQAAGKDGVQFVSIEDIFMQSHVIIVLLPFSPELENFISH
                     KYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHARN
                     IITTPHIAAATVESYQQTGIYVARSIIDFFAGREIKNVL"
     gene            complement(1886..3262)
                     /locus_tag="RH033_004304"
     CDS             complement(1886..3262)
                     /locus_tag="RH033_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HDV2993397.1"
                     /translation="MASNANFISQFVMGGDPCTYKESGELQAEMSKLTHPARPDVDWR
                     QVEKLSLALFRQNGVELQTLVCYVLAITRRQGLAGMADGLGSLDILLQRWADFWPVQV
                     HSRISLLNWVTEKMQQALRTLDIQYQDLPQIYRCVQHLSAIETTLQQCELWHMTKLDV
                     LSGQFRNAALRLERLAPQGAETIITPPELPRREMNQPKKSEESPQPVFATRSVQQNDK
                     DASPPVPSPEISRQWTWPIFMAGMVVMAGLGGTGLWGWSQLNQPDALIQRIQLSVMPL
                     PQSLESGELAKLDVKDKALLAQDRTIAASQMQLEQLNKLPARWPLEQGYRQLRQLDAL
                     WPDNPQVRALNAQWRKQRELSALSAEALNGYAQAQSQLQRLSAQLDALDERKGRYLTG
                     SELKTAVYGIRQSLKEPPLEELLRQLEEQKQTGEVSPTLLTQIDTRLNQLLNRYVILL
                     DTKVEQSQ"
     gene            complement(3262..3690)
                     /gene="tssE"
                     /locus_tag="RH033_004305"
     CDS             complement(3262..3690)
                     /gene="tssE"
                     /locus_tag="RH033_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HDV2993398.1"
                     /translation="MSTPSLYEMLTFSFSGELPLEQVSERDQLILSVMDNMQRIINCR
                     AGTLAHLPDYGLPDLSLIHQGMAAGIHGLMRQIEETLLRYEPRLSQIQVELLPQPRPG
                     HLNYLIHAQLPDTGWIRFDGVFSPEGRIVLRHLKQQERAY"
     gene            complement(3694..4230)
                     /gene="tssJ"
                     /locus_tag="RH033_004306"
                     /pseudo
     CDS             complement(3694..4230)
                     /gene="tssJ"
                     /locus_tag="RH033_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484007.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
     gene            complement(4211..5290)
                     /gene="tssG"
                     /locus_tag="RH033_004307"
     CDS             complement(4211..5290)
                     /gene="tssG"
                     /locus_tag="RH033_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HDV2993399.1"
                     /translation="MDRASQPARTGLTDRLAPDIARINFYRFCQLLEQSQQNAPLGST
                     DSPAADAVRFRPHPGMGFPVSELKNVERDVGNPDAPPTVRTTFLGLYGVDSPLPTAYL
                     DYITQRHDGHDAVMAFLDIFNHRFITQYYRIWRKYNYPASFEAGAVDDISRCLLGLIG
                     LGIPGSENHIATPVSRFLALLSVMRLPTRTAEGVTALVGLLAPLTKATVVPHDPQPVI
                     LPAPAGLSKNSRISLKTRTLLGRTGTDVNSQLLLKLYTEDAAEARGWLPGGQLHSDLL
                     VLLRVYLGWRCQARLQLTLPVSLLPAARLGKQRVQISRTGILRASFAAPATGTVTVSL
                     GRYQGLIPAFSIRNRESMTHVSYSF"
     gene            complement(5254..7014)
                     /gene="tssF"
                     /locus_tag="RH033_004308"
     CDS             complement(5254..7014)
                     /gene="tssF"
                     /locus_tag="RH033_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HDV2993400.1"
                     /translation="MDDLTQRYYEAEMRYLREAGKEFAQAYPDRAAMLNLDKPGARDP
                     YVERLFEGFAFLMGRLREKLDDDLPELTEGLVSLLWPHYLRTIPSLSVVELSTDHRQM
                     KQSETLSDFQVLSRPVGERRTRCVYSATRDITLHPLALPDVSLQYEPDGRSVIRLRFA
                     CGPLVGDWSQIDLSRLLLYLNADSPVACALHRALTLGTQQFWLRLPGQDRRMLGAHFS
                     PMGFEDDDRLWPKGESAFSGYQLLLEYFTFREKFMFVALNGLEQVAWPEGITGFEIDV
                     VLNENWPHDLPFDSDNIRLHCVPVINLFPLEADPLHLSPLENEFLLRPMRIQDGHTEI
                     YSVDNIISSRHTGSQAYVPFSSFRHRGGMLRHDAPERYYHTRVKRGPSGLHDTWLILG
                     GDAFDTDRMLEDETLSLSLTGTNGQLPRKALQSTLLDTPVHASQNVLRVRNLCAPTQP
                     CYPPARDRFHWRVLSHLGSNFLSMMDNAEILRGTLALYDWTESEMNRRRLEAIVDVQH
                     SLIQRFERGFLLRGVDIQVTLDSNGFAGEGDITLFGELLHRFFALYADIHLFTQLTLI
                     LQPTGKCLQWTEHHSQRVPG"
BASE COUNT         1689 a         1867 c         1884 g         1736 t
ORIGIN      
        1 ttaaacattt tatgcgtaac gaacataaag cagttgaaga agttattgat tccccccttt
       61 cggaggtcgc aaatttaatt aaagtattac agtcttgtca ggggaaagtt gtttttattg
      121 gtgtcggtaa gtccggtatt attgcccgaa aactcgccgc aacatttgcc agcactggaa
      181 ctccctcgtt ttttgttcac ggtactgaag ccgtacacgg cgatcttggg atggtggcga
      241 aagatgatgt tgttattctt atttcaaaca gtggtgagac gacagaaatc ctggcaacac
      301 tacctagtct gaaaaaaatg ggtaattatt tgatctcctt tacccgcagt catcgctcat
      361 cgcttgcgat aagttgtgat ttatccgttg aaattcccgt aaaaagtgag gcggataatt
      421 taggtttagc gccgtcatgc tcatcaacag tcgttctggt tgttggtgat gcggtggcgc
      481 ttgcgctctc cgaattgaaa aagtttactc gtgctgattt cggcttatat catccaggag
      541 gtgcgctcgg tattaaagca aactcataat tttagaatat aaaacagtaa ccggtcacgc
      601 catcttttgg ggtggcgttc attgatgtga gtgtcagcta tgaaaaacgt tctggtcacc
      661 gttcccagct tttcagcgcg ttgtgtttct gccagcaagc tgctaagaga aaatcatttt
      721 aaccttatta tcaagaataa tgtggagcat ctggttaaat cagaatccac agtattacga
      781 gaatctattt gcgcagtcat tgccggaaaa gattgttatc aagcggatac gttatcgcga
      841 ctaccaggag tgaaaataat atcgagattc gggacaggga ttgataatat tgatcttcat
      901 acggctcgac aatcaggtat tgtggtcaat aatgctgttg ggattaattc aaatgcagtc
      961 gcggaattta tcatcggtct tatttttgcg agcatgagaa atatccccgg tagctatcat
     1021 gcaatgcaaa atggttattg gggcgagtcg catggctgcg agttacaagg taaacgtatt
     1081 ggcctgcttg gctacggcaa tattggcaaa acactggcga aaaggttatc tggttttgat
     1141 gttgagcttt tagcttttga caaacaaccc gatttccagg ccgcaggcaa agatggagtt
     1201 cagttcgtat caattgaaga tatctttatg cagtctcatg tcatcatcgt tcttttgcca
     1261 ttttctccgg agctggagaa cttcattagt cataaatatt tgtcgatgat gcgcaatggt
     1321 gctcttatca tcaacgccgc aaggggaaag ttgctggatg agggtgcttt acttcaggta
     1381 attgaagaac gaaatgtttt tgcagcgctg gatgtgttta gctctgaacc gctagcgcaa
     1441 tttagcccat tgctacatgc caggaatatc attacaacgc cgcatattgc ggccgccacc
     1501 gttgagtcat accagcaaac cgggatatac gttgccaggt cgataataga tttctttgca
     1561 ggaagggaga taaaaaacgt gctgtgagtt cagctaagga agatttcagg tacaaaaaaa
     1621 ccgcaatttg tgaaacacat tgcggtttat cagcatcata ggtgttggtt gcgggggcca
     1681 cagtcaagag gttaatccaa ccttttgaaa cttaagacat tcactgttcc cgaacccaag
     1741 ctatctaccg aaatatacac tcattatctg atgtgaatca acgagtagat cgaatggcct
     1801 gttcctctct tttttgtaca cagagactcc ttttaaaaaa tggcaagggc acatatccct
     1861 tattggtaca taaatccccc gatgtttact gactttgttc caccttcgta tccagtaaaa
     1921 tgacatagcg attcaacaac tgatttaacc gggtatcaat ttgcgtcaac agcgttggcg
     1981 aaacctctcc ggtctgtttt tgctcttcca gttgccgaag cagttcttcc agcggcggct
     2041 cctttaacga ctgccggatg ccgtacaccg ccgttttcag ttccgaaccg gtcagatatc
     2101 tccccttacg ttcatccagt gcatccagct gcgccgacag gcgctgtagc tgactctgcg
     2161 cctgagcata gccattcagt gcctcagcag acagggcgct cagctcccgc tgtttgcgcc
     2221 actgcgcgtt cagcgccctg acctgaggat tatccggcca cagggcatca agctggcgta
     2281 gctggcgata tccctgctcc agtggccagc gggcaggcaa tttgtttaac tgctccagtt
     2341 gcatctgact tgccgcaatt gttctgtcct gagccagcag cgccttatcc tttacatcca
     2401 gctttgccag ttcgccgctc tccagcgact gcggcaacgg catgacagac agttgtattc
     2461 gctggattag cgcgtccggc tgattaagct gcgaccagcc ccataatcct gttccgccga
     2521 gaccggccat cacaaccatt ccggccataa atatcggcca tgtccactgc cgggagattt
     2581 caggggatgg cacgggtgga ctggcatcct tatcgttttg ctgaacggat ctggttgcaa
     2641 aaaccggctg tggactttcc tctgactttt tcggttgatt catttcccgg cggggtaatt
     2701 cagggggagt gataatggtt tccgctccct gaggcgccag ccgttccaga cgcagagccg
     2761 cattgcgaaa ctgcccggac aatacgtcca gcttcgtcat atgccacagt tcacactgtt
     2821 gcagcgtggt ttcgatggcg gaaagatgct gtacacaacg gtagatctgc ggcagatcct
     2881 gatactgaat atccagcgtt ctcagtgcct gctgcatttt ttctgtgacc cagttgagca
     2941 gtgatattct ggaatgtacc tgtaccggcc agaagtcagc ccagcgctgg agcagtatat
     3001 ccagtgaacc gagtccgtct gccatccctg ccagcccctg ccgtctggtt atcgccagta
     3061 cgtaacagac cagcgtctgt agttccacgc cattttgccg gaacagcgcg aggctgagtt
     3121 tttccacctg acgccagtcc acatccggtc gggccgggtg agtcagttta ctcatttcag
     3181 cctgcagttc accggattcc ttataagtac aaggatcgcc gcccatgacg aactggctga
     3241 taaaattcgc gttacttgcc atcagtacgc ccgctcctgc tgtttgagat gacgcagaac
     3301 aattcgtcct tccggagaaa atacgccatc aaagcgtatc cagccggtat cgggaagctg
     3361 cgcgtggatc aggtaattaa gatgccccgg acggggctgg gggagtaatt ccacctgtat
     3421 ctgactcagg cgtggttcat aacgcagcag tgtttcttca atctgacgca tcaggccatg
     3481 aatacccgca gccatcccct gatggatcag actgagatcc ggcagaccgt aatccggcaa
     3541 atgcgccagc gtgcctgccc ggcagttgat aatgcgttgc atattgtcca tcacggaaag
     3601 gataagctga tcccgctcgc tgacctgttc cagcggtaac tcgccgctga aactgaacgt
     3661 cagcatttca tataaggaag gtgtcgacat cggttattta tccttgagag gcagcagtcg
     3721 caggtcgctt ctcatcagtt caatcgagcg cggcttgtcg gcctccagtt cgtccctttt
     3781 gatcaccaga cgccagctgt cgcttttttc atcagggtga taaaactgcc caataatggc
     3841 gacaaactgc gtctctttat ccagcggcac attcagggat gccgtgcctt ccggttttac
     3901 ccagacggtg tgttttgcca gtacgtcctt ctccagcgtc ttctcttcct gcataaacag
     3961 ggtgtcgtaa tccgccttgt caaaatcttc gcgggttttc agttgtcaga catgcaccat
     4021 cgtggaaagc ggcgtatctt ccgcatccgt attcagggcg ctgcgggaga agaaatcgag
     4081 atgcagtatt ttgatttgtt tgtagaaaac ggctgaggcc acgcttttcg attcatccac
     4141 cactttctga atcagcccgc agccactcag aacaaccgtc agcatcgcca tacagagcaa
     4201 cgccggacgt ttaaaacgaa taggaaacat gagtcatact ctccctgtta cgaatggaaa
     4261 aagcgggtat taatccctga taacgcccca gactgaccgt cactgttccg gtcgcgggcg
     4321 cggcaaagga ggcgcgaagg atgccggtac ggctaatctg tacacgctgt tttcccagcc
     4381 gggcagcagg caacaggctg acgggcagcg tcagttgcag acgggcctga cagcgccagc
     4441 ccagataaac gcgcagcagc accagcaaat cgctgtgcag ttgcccgccg ggcagccagc
     4501 cacgcgcttc tgcggcatct tcggtataca gtttcagcag caactggctg ttgacgtcgg
     4561 taccggtacg ccccagcagc gttcgcgttt tcaggctgat gcgtgaattt tttgacagac
     4621 ccgcaggggc cgggagaatt acaggctgcg gatcatgagg caccaccgtc gctttcgtca
     4681 gcggggcaag cagtcccacc agtgccgtaa cgccttccgc cgtgcgcgtc ggcaggcgca
     4741 tcacgctgag cagggccaga aaacgggata ccggcgtggc gatatggttc tcgctgccgg
     4801 gaatacccag cccgatgagt cccagcagac aacgggaaat gtcatccacg gcacctgctt
     4861 caaacgacgc cgggtagtta tatttgcgcc agatgcggta atactgggtg ataaagcggt
     4921 ggttaaaaat atcgagaaac gccatcactg cgtcatgtcc gtcatgacgc tgggtaatgt
     4981 agtccagata ggcggttggc agcggcgaat ccacgccgta caggccgaga aaggtggtgc
     5041 gcaccgtggg cggcgcgtcg ggattgccca cgtcacgttc aacgttcttt aactcgctga
     5101 ccggaaagcc cattccggga tggggacgaa agcgcaccgc atcggcggcg gggctgtcgg
     5161 tactgcccag cggcgcgttc tgctggctct gttccagcaa ctggcaaaaa cggtaaaaat
     5221 tgatccgcgc aatatccggg gccagccggt cggtcagccc ggtacgcgct ggctgtgatg
     5281 ctctgtccat tgcaggcact ttcctgtggg ttgcagaatt aaggtcagtt gagtgaataa
     5341 gtggatatcg gcatacagtg cgaaaaagcg gtgcagcagt tcaccaaaca gcgtgatatc
     5401 accttctccg gcaaagccgt tactgtccag cgtcacctga atatccacgc cacgcagcag
     5461 aaagcctctt tcaaaacgct gtatcaggct gtgctgaacg tcgacaatcg cctccagacg
     5521 acggcggttc atctcacttt cggtccagtc gtacagtgcc agcgtgccgc gcagaatttc
     5581 ggcgttatcc atcatcgata aaaagttaga accaaggtga ctgagcaccc gccagtggaa
     5641 gcggtcacgc gcagggggat agcagggctg cgtcggagcg cacaggttgc gcacccgcag
     5701 gacgttctgt gaggcatgaa ctggcgtatc gagcagcgtg ctctgtaaag ccttacgggg
     5761 aagctgcccg ttagtaccgg tcaggcttaa agagagcgtt tcatcctcca gcatccggtc
     5821 agtatcaaac gcatcgccgc ccagaatcag ccaggtgtca tgcaggccgg aagggccgcg
     5881 ctttacccgc gtgtggtaat agcgttccgg cgcatcgtgg cgcagcatcc cgccccggtg
     5941 acgaaagctg gagaagggga catacgcctg actgccggta tggcgcgaag aaataatgtt
     6001 gtcgacggaa taaatctcgg tatgcccgtc ctgaatgcgc atcgggcgca gcagaaattc
     6061 gttttccagt ggcgaaaggt gtaacggatc ggcttcgagc gggaacaggt tgatgaccgg
     6121 cacgcagtgc agacggatat tatcgctgtc aaacggcagg tcgtgaggcc agttctcgtt
     6181 cagcaccacg tcaatctcaa agccggttat cccctccggc catgcaacct gctccagccc
     6241 gttcagcgcc acgaacataa acttctcgcg gaaggtgaaa tactccagca gcaactggta
     6301 accgctgaag gctgactcac cttttggcca gagcctgtca tcatcctcaa agcccatcgg
     6361 ggaaaagtgt gcgccaagca tccggcgatc ctgccccggc agacgcagcc agaactgctg
     6421 cgtgccaagc gttaaagccc ggtgcaacgc acaggctacc gggctgtcgg cattgagata
     6481 gagcagcagg cgggataaat cgatctgcga ccagtcacca accagcgggc cgcacgcaaa
     6541 gcgcagacgg ataaccgagc gtccgtccgg ttcatattgc aatgacacgt ccggcaacgc
     6601 cagcggatgc agcgtgatat cacgcgtggc actgtagacg cagcgggttc tgcgctcgcc
     6661 aacagggcgc gacaatacct gaaaatcgct cagcgtttcg ctctgcttca tctgccggtg
     6721 atctgtcgac agttcaacca cggaaagtga cgggatggtt cgcaggtagt ggggccacag
     6781 caggctgacc agcccttcag tcagttccgg cagatcgtcg tccagctttt cgcgcaggcg
     6841 gcccattaaa aaagcgaagc cttcgaacag gcgctccaca tagggatcgc gtgcgccggg
     6901 tttatcaaga ttgagcatcg cggcgcggtc ggggtaagcc tgcgcaaatt ctttccccgc
     6961 ttcacgcagg tagcgcattt cggcttcgta gtagcgttgg gttagatcgt ccatcggagg
     7021 gattatcttc ctttcattga aagtgaatat catgcaggtg tagaagtaac atcatagtta
     7081 aatttattct atgtgaatat attatggata gttgctgact tattgatttt aataatgatg
     7141 tattcttgtc ttttctgaac caaattaaat ttaccg
//
LOCUS       DAPHCV010000171         7130 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.173, whole genome shotgun sequence.
ACCESSION   DAPHCV010000171 DAPHCV010000000
VERSION     DAPHCV010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.173"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..581
                     /locus_tag="RH033_004309"
     CDS             <1..581
                     /locus_tag="RH033_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TcpQ domain-containing protein"
                     /protein_id="HDV2993401.1"
                     /translation="TLLTGQQLYGHMDWNRKILTVTTTATPPVDLTAPQGSQKTADTP
                     RNPFRSTTGTAADQAQVPPAVSASRVQSTTGTSGTSAPRTTTPAPVVTKPVAPPQKVT
                     PPRQWKMEKGTTLKDGLARWTSSEKCSHGTWTLRWETPVNYSIDAPLVFTGSFRQALE
                     QLFALYTPVEKPLYARTSTAQCLLRVTDRPQE"
     gene            586..1023
                     /gene="pilM"
                     /locus_tag="RH033_004310"
     CDS             586..1023
                     /gene="pilM"
                     /locus_tag="RH033_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HDV2993402.1"
                     /translation="MGWFIVFIMVITTTGNFWFTSQLSRSEHRHQAAENTQQAATFIR
                     YMNAINDYLHQHPERRTAGGRLTSAQLGIPGTDTVSHIISQQRVFVWATETPGLMAAL
                     REQSNDSALLGRVENGRLLDTAGRALSVTLPSVIPDHVILWMN"
     gene            1054..2673
                     /locus_tag="RH033_004311"
     CDS             1054..2673
                     /locus_tag="RH033_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HDV2993403.1"
                     /translation="MNKKILAIMIAAALLQGCASYRQLESTRKEAVAEQQRAREMMSD
                     LRTRTPVVKESSRQWINPRPVMDKNTGKTVPGCPIVIHTRQSITLQQVVQRITESCHI
                     PVRITPDVLTYLNTSTTGSTQQIQGALPPPDASGMVPLAAIGASDTPPSRLPGNTAAL
                     SELNASGLTTLLNAVSSRLGISWRYDGSHIIFYYLETQTFPITYMDSQVAYNATVVSG
                     TMSSNGSSGGSASDAISGDASNTQTTTVDMKSALYQDVKNAVTSMLTPGIGRMFMSTG
                     FITVTDNPQVLETVRAFIEKRNEEMKRQVVLNVEILSIRKTRNEQAGIDWNAVFSDRT
                     LGLSLGSTFTSAASDAITGGVSIVDGKLTGSKAFLKALSSQGDVSVVTQNSAVTKNLT
                     PVPMQIANQQSYIESVTTDTTANVGSSTSLNAATITTGFNMTLLPFILPDSQTLQLLY
                     SMSLSDKPVIENYESGGSKAQLPNVDLKTINQTVDLKSGQTVIISGFQQSGRRSGKQG
                     VGTPGFFGLGGGINSENDDTILVVLITPNII"
     gene            2694..3989
                     /gene="pilO2"
                     /locus_tag="RH033_004312"
     CDS             2694..3989
                     /gene="pilO2"
                     /locus_tag="RH033_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HDV2993404.1"
                     /translation="MTDISPTGRQVFPLTAGRNVFLAGLEWKTLPSQYRHARDFARAQ
                     KADLFLACQYLSNEDADTHTMVATVSRRILPGKPRQCFSLALLILPLLEHGGYAITEL
                     TLPGETPHYSFVSVVDGVLVSDLVGSGEEVREARDTFLSINTEPTQGWIHYEPAAFSA
                     GAQNQHLPLASLTGSGKHPAAARLNPVSRGPQFITVSLIIALLGTAWYGWQYYERWQT
                     EKAAQAAVARQATEERITPPWPGLPETDAFIRGCADIWTSLPLSAAGWRYSLAECSTD
                     GGSGNLRASYTNTAGATVTDFIRRISDMTDSRPFIVMPEGKTGGIGLPVTFRLSENAV
                     TVDALPETAVLQERLTSLAQSMQLKLDWQEVNNSVTDENGQIIQPPWKEYDLQIQTTL
                     PAHHLAERLKEPSVRFISVTRQLENGRFHYQFTGKYYAR"
     gene            3979..4431
                     /gene="pilP"
                     /locus_tag="RH033_004313"
     CDS             3979..4431
                     /gene="pilP"
                     /locus_tag="RH033_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HDV2993405.1"
                     /translation="MPDKAKTLLFSVLLLPATAMATTGNAEQQNTQQIRIEQIQEETA
                     LYEAMLAREKIRHELRTLEQGGPAEAGRQQESISAVTTNTQASLSPRLTAIFGVQPQL
                     TARIELPGGSISDVKAGQNIPGTTYHVEKITPDSVVLEQSGTRKTLRP"
     gene            4516..6024
                     /gene="tadA"
                     /locus_tag="RH033_004314"
     CDS             4516..6024
                     /gene="tadA"
                     /locus_tag="RH033_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HDV2993406.1"
                     /translation="MLDPADLPPDDILDYVAIDTDNAGQLRIRVVEGKKHLRAVQEYL
                     TRLRARYQGRVGDFEFTTLDVIARIREATTIAGQENGMNPVQNKMLGYIRHAATLNAS
                     DLHITPGRDNTDFTYVEARVHGELEILDIIRKDEGVELLGATYSGMTDVIKGTQFDPG
                     VPQDARLAEKFLKLAGLFGARYSHYPCVGGLYAVLRLIKDDSSHIPTFGMLGYHPKQE
                     QTIRRILQRPEGIITLSGPTGSGKSTTLRTASAAYLEQHGFNNTGGILLPRRRLFTIE
                     SPPEGRIPGAIQTAVMDSAQGWVDSIKSALRLDPDGILNGEIRDHDSAITAIKAAMTG
                     HLMLTTIHANDPINILERLEMEGVQARMIADPQLFIGLLSQRLVQLICPHCRHPWNDV
                     ASRRTEDERRLVETFCNADAVYLRNHDGCPHCWRGVTGRTVIAEVISPDARFFQLYRE
                     KGRIEAKTYWHRELGGMTRNQHLLGKINSGLVDPLAAHYISPIDEDSYTLLR"
     gene            6026..7126
                     /locus_tag="RH033_004315"
     CDS             6026..7126
                     /locus_tag="RH033_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDV2993407.1"
                     /translation="MKFLEKLNSTELDLSALNSWTARKTFGLKRAEFYESLGGMIRDG
                     TPSLRALEFICNVETDFGTRKGTSGMYFLARECIASLKASGMLSGALPTWVPPEEAAL
                     IRNGEERGNIAEALFQVAKTVKARREMVSSLITLCLYPLLLLSLCVVNMYNAHTRIIP
                     IVSAFAPEEKWSFQMKVLAAMSELVIGYGLWLSGAIITLAIIIRFSLSRYTGPGRRLL
                     DRLPPWSLYQTFHGVNYLFSISSMLQINIPLSQALLRIEKSAEHNKWLKERIQAIRKN
                     VLSGQNLATAMRNSDYDFPSKICINKLLLNSEREDSVSAMNTYAGQWLEDAKKSVRKS
                     GLIITGISGGFVLFFILNMISAIYSISDMLKQ"
BASE COUNT         1800 a         2018 c         1826 g         1486 t
ORIGIN      
        1 atactctgct gaccgggcag caactgtacg gacatatgga ctggaacagg aaaatactga
       61 ctgtcacgac cactgcaaca ccgcctgttg acctcacggc accgcagggt tcacagaaaa
      121 cagcggacac accacgtaat ccgttcagga gcacaaccgg aacggctgca gaccaggcac
      181 aggtaccacc tgccgtcagc gcttcccggg ttcagagcac aacagggaca tccggtacat
      241 ctgcgccccg cacaaccacg ccagccccgg tggtcacgaa accggtagca ccaccacaga
      301 aggtcacacc gccacggcag tggaaaatgg aaaaagggac cacgctgaag gacggactgg
      361 cccgctggac gtcgtccgaa aaatgctccc acggcacctg gacgctgcgc tgggaaacgc
      421 ccgttaacta cagtatcgat gcccctctgg ttttcaccgg cagtttccgc caggcgctgg
      481 aacagctctt cgcactctac accccggtgg aaaaaccgct gtatgcccgg acaagcacag
      541 cacaatgtct gctgcgggtg acagatcgtc cacaggaata accttatggg ctggttcatc
      601 gtgttcatca tggtcatcac gaccacaggc aatttctggt tcaccagtca gctctcccgc
      661 agcgaacacc gtcatcaggc cgcagaaaac acacaacagg cagcaacgtt cattcgttat
      721 atgaacgcca tcaatgatta cctgcatcag catccggaac gacggacagc cggagggcgg
      781 ctgacatccg ctcagctcgg gattcccggg acggataccg tcagtcatat catcagccag
      841 caacgtgttt ttgtctgggc tacagaaacc ccgggcctga tggccgcgct cagggaacag
      901 agtaatgact ctgctctgct cggcagggtg gaaaacggcc gactgctgga cacagccggc
      961 agagcgcttt ccgtcacact tccgtcagtg atccccgatc acgtcatcct ctggatgaat
     1021 tagtcacgaa tctgaataaa tcacggatct cccatgaata aaaaaattct tgccattatg
     1081 attgccgccg ctctgttaca gggctgtgcc agttacagac agcttgagag cacaagaaag
     1141 gaagccgttg cggaacaaca acgggcacgg gaaatgatgt ctgatctgcg aacccggacg
     1201 ccggttgtta aggaatcctc acgacaatgg atcaatcccc gtccggtcat ggataaaaac
     1261 accggcaaaa ccgttcccgg ttgtcccatt gtcattcata cccgtcagag catcaccctt
     1321 cagcaggtgg ttcagcggat cacggaaagc tgccatattc cggtacggat cacacctgat
     1381 gtactgacat acctgaatac atccacaacc ggcagcacac aacaaatcca gggcgcatta
     1441 cccccaccgg atgcgagcgg catggttccc cttgccgcca ttggcgcatc tgacacgcca
     1501 ccatcccgtc tgccgggaaa taccgcagca ctgtctgaac tgaatgcctc cggcctgacg
     1561 accctgctga atgcggtctc ctcccgtctc ggtatatcct ggcgctatga cggctcgcat
     1621 atcatcttct attatctcga gacacagacc ttccccatca cctatatgga cagtcaggtg
     1681 gcctataacg ccactgtggt cagcggcacc atgtcctcaa acggctcatc aggaggcagt
     1741 gcatcagatg ccatttccgg tgatgccagc aatacccaga ccaccaccgt ggatatgaag
     1801 tctgcgctgt atcaggatgt gaaaaatgcg gtcacatcaa tgctgacccc gggtatcgga
     1861 cgcatgttca tgtccacggg ctttatcacc gtcacagata atccacaggt tctggaaacg
     1921 gtccgggcat tcattgaaaa acgcaatgaa gagatgaaac gtcaggtggt gctgaatgtg
     1981 gaaattctga gtatccggaa aacccgcaat gagcaggccg gtatcgactg gaacgcagtc
     2041 ttcagcgaca gaacgctggg actgagtctc ggcagcacat tcaccagtgc agccagtgat
     2101 gccatcacag gtggtgtttc cattgtggac ggtaaactca ccggttcaaa agccttcctg
     2161 aaagcattgt cttcccaggg ggatgtcagt gtggtgaccc agaactctgc cgtcaccaaa
     2221 aacctgacgc cggtgccaat gcagatcgcc aatcagcaaa gttacatcga gagtgtgacg
     2281 acggatacca cagccaatgt cggctcatcc acctccctga atgcggccac catcaccacc
     2341 ggttttaaca tgacccttct gccgttcatt ctcccggact cacagacact gcagttgctc
     2401 tattccatga gtctctccga caaaccggtt attgagaatt atgagagcgg tggaagtaaa
     2461 gcccagctgc cgaatgtgga tctgaaaacc atcaatcaga ccgtggatct gaaatccggg
     2521 cagacggtca ttatcagtgg cttccagcag tcaggacgtc gcagcggaaa acagggtgta
     2581 ggcacgccgg gattcttcgg tcttggcggt gggatcaaca gtgaaaacga cgacaccatt
     2641 ctcgtggtgc tgatcacgcc caatatcatc tgatttgcac aaggaggacg atcgtgacgg
     2701 atatttcacc gacagggcgg caggtgtttc cgctgacggc agggcgcaat gtgtttctgg
     2761 ccggtctgga atggaaaaca ctgccctcac agtaccggca tgcccgcgac tttgcccggg
     2821 cacagaaagc cgaccttttc ctggcctgtc agtatctcag taatgaagat gccgataccc
     2881 acaccatggt agccactgtc agtcgccgca tactgcccgg aaaaccccgt cagtgcttct
     2941 ccctcgccct gctgatcctg ccactacttg aacatggcgg ctatgccatc acggaactga
     3001 cgttacccgg agaaacaccg cactatagct ttgtttccgt tgtcgacggt gttctcgtca
     3061 gtgacctggt gggaagcggg gaagaggttc gggaagcccg ggataccttt ctttccatca
     3121 acaccgaacc gactcagggc tggatacact atgaaccagc ggcattttca gcaggcgctc
     3181 agaatcagca cctgccgctg gcctccctga ctggctctgg taaacatccg gctgccgcac
     3241 gcctgaatcc ggtatcccgg ggaccgcagt tcatcactgt ctctctgatc atcgcccttc
     3301 tcggcactgc atggtacggc tggcagtatt atgaacgctg gcagacagaa aaagccgcgc
     3361 aggcagcggt tgcccgacag gccacagagg aacgaatcac tcccccctgg cccggactcc
     3421 ctgaaacaga cgcgttcatc cggggatgtg ctgatatatg gacttcactg ccactgtctg
     3481 ctgccggatg gcgttattcg ctggcagaat gttcgactga cggtggcagc ggcaatcttc
     3541 gcgcatcgta caccaatact gcgggcgcga cagtgactga cttcatccgg cgcatcagtg
     3601 atatgacaga cagccgaccg tttattgtga tgccggaagg aaagaccggc ggcattggcc
     3661 tgccggtcac cttccggctg tctgaaaacg ccgtcacagt tgatgcacta ccggaaaccg
     3721 ctgtcctgca ggaacgactg acctcgctgg ctcagtcaat gcaactgaaa ctggactggc
     3781 aggaagttaa taacagcgtc actgatgaga acggacagat cattcagccc ccatggaaag
     3841 agtatgacct tcagatacag accacactcc ctgcacacca tctggctgaa cgcctgaaag
     3901 agccttctgt ccgttttatc tctgtcacac ggcaactgga gaacggtcgt ttccactatc
     3961 aatttacagg taaatattat gcccgataaa gccaaaaccc ttctgttttc agtcctgctg
     4021 ttaccggcca cggcaatggc cacaaccgga aatgcagagc aacagaacac acagcaaata
     4081 aggattgaac agatacagga agagacagcc ctgtatgaag ccatgctggc aagagagaag
     4141 atccgccatg aactgcgaac acttgagcag ggggggcccg cagaggccgg acgtcagcag
     4201 gaaagcattt ccgcagtcac aacaaataca caggcatcac tatccccccg cctgacagca
     4261 atattcggtg ttcagcctca gctgactgcc cggatagaac ttccgggggg aagcatctcc
     4321 gatgtgaagg cgggccagaa catccccgga accacttatc acgtggaaaa aatcacgccg
     4381 gacagtgtgg tgctggaaca gtctggcacc agaaaaaccc tgcgaccatg agaccggccg
     4441 ccgtaactgg cggctgtcaa aaataaaagg atgaaagaca tattttccgg gcaccgctgc
     4501 ccgtaaggag gctttatgct ggatccagca gacctgccgc cggatgacat tctggattat
     4561 gtggccatcg acaccgataa cgccggacaa ctgcggatca gggttgttga gggaaaaaaa
     4621 catctgcgtg ccgtccagga atatctgacc cgcctgcgcg cacgctatca gggacgagtc
     4681 ggagactttg aattcaccac actggatgtg atagcccgga tccgggaagc aacaacaatc
     4741 gccggacagg aaaacggcat gaatcctgtg cagaataaaa tgctcgggta tatccgacat
     4801 gccgccaccc ttaatgcctc cgatcttcat atcactccgg gccgggacaa cacagacttc
     4861 acctatgtgg aggcccgtgt acatggggaa ctggaaatcc tcgacattat ccggaaagat
     4921 gagggagtgg agcttctggg ggccacctac tccgggatga ctgacgtcat caagggaaca
     4981 caatttgatc ctggtgttcc ccaggacgcc cgactggcgg aaaagtttct gaaacttgcg
     5041 gggttgtttg gcgcacgtta ctcccattat ccctgcgtgg ggggccttta tgctgttctg
     5101 aggctgatca aggacgacag cagccatatt ccgaccttcg gtatgctggg ctatcatcca
     5161 aagcaggagc agaccatccg ccgtattctg caacgaccgg aaggtatcat caccctgtca
     5221 gggccgaccg gctccggaaa atccaccacg ctgaggacgg cgtctgcggc ctatcttgaa
     5281 cagcacggat tcaacaacac cggtggtatt cttttgcccc gtcgtcgtct cttcaccatt
     5341 gaatcaccgc cggaaggacg tattcccggc gcaatccaga ccgccgtcat ggacagtgct
     5401 cagggctggg tggattccat aaaatcagcc ctgcggcttg atcctgacgg cattctgaac
     5461 ggtgaaatcc gcgatcacga ttccgccatt acggcaataa aagccgccat gacaggccat
     5521 ctgatgctga ccaccatcca tgcgaacgac ccgattaaca tcctcgaacg ccttgagatg
     5581 gaaggtgttc aggccaggat gattgcagac ccgcagttgt tcatcggact gctcagtcag
     5641 cgtctggttc agctgatatg tccccactgc cgtcatccat ggaatgacgt ggcatcccgt
     5701 cgtactgagg atgaacgacg tctggtggaa accttctgta atgctgatgc ggtatatctg
     5761 agaaaccatg acggctgccc acactgctgg cgtggtgtca cagggcgcac agtgattgca
     5821 gaagtcatct ctcctgatgc cagatttttt cagttgtacc gggaaaaagg ccgcatcgaa
     5881 gccaaaacct actggcacag agagctgggc gggatgacgc gtaatcagca tctgctggga
     5941 aaaatcaaca gcggactggt tgaccctctg gcggcccatt acatctctcc cattgatgag
     6001 gacagctata cgctgctgcg ctgatatgaa atttctggaa aaactcaaca gtactgaact
     6061 ggatctgtct gccctgaaca gctggacagc cagaaaaacc ttcggtctta agcgtgccga
     6121 gttctatgag tccctcggcg gaatgatacg tgacggaacg ccgtcactgc gcgccctgga
     6181 gtttatctgt aatgtcgaaa ctgactttgg cacccggaaa ggaacatccg gcatgtactt
     6241 tctggccaga gaatgtattg cctccctgaa agccagcggt atgctgtcgg gggccctgcc
     6301 cacatgggtt cccccggaag aagcggcact gatcaggaac ggtgaagaac gcggaaacat
     6361 cgccgaagcc ctgttccagg tcgcaaaaac cgtgaaagca cggcgggaaa tggtgagcag
     6421 tctcatcaca ctctgtcttt atccccttct gctgctttca ctctgtgtgg tgaatatgta
     6481 caacgcacat acccgcatca ttcccattgt cagcgctttt gcaccggagg agaaatggtc
     6541 attccagatg aaagtccttg cagccatgtc agagctggtc atcggctacg gtctgtggct
     6601 cagtggtgcc atcatcacgc ttgccatcat catccgcttc tcactcagtc gctataccgg
     6661 cccgggacgc agacttcttg acagactgcc accatggtcg ctctatcaga cttttcatgg
     6721 tgtgaattat ctgttcagca tatcgtcaat gctgcagata aatattccgc tttcacaggc
     6781 gctgctcaga attgaaaaaa gtgcggagca taataaatgg ttaaaggaga gaattcaggc
     6841 aataagaaaa aatgtattat ccggtcagaa tctggccacc gccatgcgca attctgacta
     6901 tgattttcct tcaaaaatat gcatcaataa acttctctta aattctgaaa gggaagacag
     6961 cgtatctgca atgaatacct atgccggaca gtggctggaa gatgcaaaaa agagcgtcag
     7021 aaaaagtgga ttaattatca ccggtatttc aggaggtttt gtattatttt ttattcttaa
     7081 catgattagc gcgatataca gtatcagcga tatgctcaag caataatacc
//
LOCUS       DAPHCV010000172         7029 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.174, whole genome shotgun sequence.
ACCESSION   DAPHCV010000172 DAPHCV010000000
VERSION     DAPHCV010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.174"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(169..876)
                     /locus_tag="RH033_004316"
     CDS             complement(169..876)
                     /locus_tag="RH033_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV2993408.1"
                     /translation="MKTFAERLNAAMSSAGVSQSQLADMVGISQPAIQKMSSGKTNGS
                     RKMVELANALKVRPEWLSSGIGEMRDGAHEEPSNVRESSLKAVVWEDIKRNDDEFVAL
                     PLLNVSLSAGSGSCELEESSEFSLVFRKHYLKKMGVSERSAKLVRVVGQSMEPTLHDG
                     DVVGVNTQDTTIRDGKTYAICQSDLLRVKTLIATPTSVIIRSINREEYPDEVMDRDEF
                     HETVRIIGRVFWSSHSW"
     gene            988..1212
                     /locus_tag="RH033_004317"
     CDS             988..1212
                     /locus_tag="RH033_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV2993409.1"
                     /translation="MNLVIQRALKIVGSQKRLADKCGVTQPAVHKWLKGGLVSPEKVT
                     AIVNATGGQIKAYEIRPDLPHLFPKPNQAA"
     gene            1322..1600
                     /locus_tag="RH033_004318"
     CDS             1322..1600
                     /locus_tag="RH033_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001177654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HDV2993410.1"
                     /translation="MELTSTRKKANAITSNILNRIAVRGQRKVADALGINESQISRWK
                     DSFIPKMGMLLAVLEWGVEDEELAELAKKVARMLTKEKAPKNGEFFEA"
     gene            1783..2673
                     /locus_tag="RH033_004319"
     CDS             1783..2673
                     /locus_tag="RH033_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HDV2993411.1"
                     /translation="MGVVKLADYRHNPVQHQETSSMGYVSIHRQFMDSRLYKDSQAVH
                     LWLHLILKANHESTVVNTDIGPITVDRGQMITGRPSLVRETFIPDNKVRSLLRTFESK
                     GMLNICSMGKKFSLFTIVKYDDFQAKNCPTVVQRLSNANTSNGAALSGDCPTVVQRLS
                     INNNINNISNTDVLESATADKKSDKKKPSVSCQDVVDAYHEILPEAPRIRALNDKRKN
                     QIRTFWRKAGVITRQLDGHGFTMQDWRNYLSYVGENCRWMFEERPNHQRGTVWHKKGF
                     DFLLNDNTYLKVREGEHDDR"
     gene            2663..4099
                     /locus_tag="RH033_004320"
     CDS             2663..4099
                     /locus_tag="RH033_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDV2993412.1"
                     /translation="MTDNFYAPPHSIEAEQAVIGGLLLDDDSSERVQKVLAMLKPDSF
                     YSRPHKILFEEITRMHREQKPVDGLTLFDELERKSLTASVGGFAYIAEIAKNTPSAAN
                     IVAYAMQVRETAMERYAINRMTEATELLYSRNGMTATQKYEAIQAIFTQLTDHAKTGS
                     RRGLRSFGEVMEDWVSDLEKRFDPSGEQRGMSTGIPSLDRMLSPKGLVKGSLFVIGAR
                     PKMGKTTLYSQMAINCAVHEKKPALMFSLEMPGDQILEKLVGQKSGVNPNIFYLPATN
                     DADDGYQGDYDGDFNRAIETANRLSEIDMLYIDDTPGLSLAQIVSESRRIKREKGCVG
                     MILVDYLTLMTAEKADRNDLAYGMITKGLKNLAKELDCVVVLLTQLNRALESRTNKRP
                     LPSDSRDTGQIEQDCDYWVGIHREGAFDDSVPPGETELILRLNRHGNTGTVYCIQANG
                     AIYDTDQQSAEMRRREREEPQSKKKGGF"
     gene            4175..4381
                     /locus_tag="RH033_004321"
     CDS             4175..4381
                     /locus_tag="RH033_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993413.1"
                     /translation="MVEFMLVALKCVGVGWILLTFFIVLHSYIRLVNDGKDPWYTLFG
                     AAFVWVIIGVMPVAVAKMAWRFVS"
     gene            4399..4713
                     /locus_tag="RH033_004322"
     CDS             4399..4713
                     /locus_tag="RH033_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993414.1"
                     /translation="MDESRKQFEEYVAKKLRLPFEMITEARNGDRYFAFSSMDIRHSL
                     NEWWTLWQASRAAIEITAPKFIDSREALAKGFTVDYSNGFGDGMDAYEENIRAAGIKV
                     KE"
     gene            4913..5323
                     /locus_tag="RH033_004323"
     CDS             4913..5323
                     /locus_tag="RH033_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HDV2993415.1"
                     /translation="MKQTIFLRTKQQQQAAINAILATPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMVADIARQVQWRDKWLKPEQWKVLLISGHAVATKQEADVLPGLEGEYVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            5320..5496
                     /locus_tag="RH033_004324"
     CDS             5320..5496
                     /locus_tag="RH033_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HDV2993416.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRYRARK"
     gene            5499..5840
                     /locus_tag="RH033_004325"
     CDS             5499..5840
                     /locus_tag="RH033_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2591 family protein"
                     /protein_id="HDV2993417.1"
                     /translation="MDYSQLSDFEINRMVGDIIFKGLWASKPETSGNNTNKWYYGNAD
                     TTFEPLNHLPDYCNDPSASWPIIEKYRISILDQLTEWCVDAKGVSPIFDARPLRAAMI
                     VFLLMQEANNA"
     gene            5961..6050
                     /locus_tag="RH033_004326"
                     /pseudo
     CDS             5961..6050
                     /locus_tag="RH033_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
     gene            6047..6409
                     /gene="rusA"
                     /locus_tag="RH033_004327"
     CDS             6047..6409
                     /gene="rusA"
                     /locus_tag="RH033_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HDV2993418.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYCDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKASFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            6406..6546
                     /locus_tag="RH033_004328"
     CDS             6406..6546
                     /locus_tag="RH033_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HDV2993419.1"
                     /translation="MMFEFNMAELLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            6632..7015
                     /gene="quuD"
                     /locus_tag="RH033_004329"
     CDS             6632..7015
                     /gene="quuD"
                     /locus_tag="RH033_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HDV2993420.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
BASE COUNT         2026 a         1524 c         1701 g         1778 t
ORIGIN      
        1 attagatgct cttcttcttt tgcgcgatta gagctatatt caatcatttg agatagcttt
       61 ttgctaatgc ttagcaaaac aaaaagaatg atgaccaaaa caacaacaaa aactatcaga
      121 aattccatta tccctccgca cttccgtaag tcttcttctg attatcggtt accaactatg
      181 agacgaccag aatactctgc caataatcct tacggtttca tgaaattcat ctctatccat
      241 tacttcatcc gggtactctt cgcgatttat tgatctgatt atcaccgatg taggggtggc
      301 gattaatgtt tttactcgta acaaatcaga ctggcaaata gcgtaggttt taccatctct
      361 gattgtggta tcttgcgtgt taacaccaac aacatcgcca tcgtgaagag ttggttccat
      421 gctttgccct acaaccctaa ctagcttggc tgatctttct gatactccca tctttttcag
      481 atagtgcttt ctgaaaacca aagagaactc cgatgattcc tctagctcgc agctaccgct
      541 tccagctgaa agcgaaacgt taagaagagg caacgcaaca aactcgtcat cgtttctttt
      601 aatgtcttcc cataccacag cttttaaaga tgactcacgg acattggatg gttcttcatg
      661 tgcaccatcc ctcatttcac caataccaga actaagccat tcaggacgca cttttaaagc
      721 attggctaat tcaaccatct tgcgagatcc gtttgtttta ccggacgaca tcttctgtat
      781 ggctggctga gatattccaa ccatgtcagc aagctgtgat tgtgataccc ctgctgagct
      841 catggctgca tttagtcttt ctgcgaatgt tttcataccc acaaatctat aactacggtt
      901 atccaaagta aaataacaaa ggttattgct attttttata acttgagtta tctttggtta
      961 taagtaatga ccacaagagg tatgctcatg aatttagtaa ttcaacgagc cttgaaaatt
     1021 gtcggtagcc aaaagcgcct tgccgacaag tgtggtgtaa cgcagccagc agtacacaaa
     1081 tggctgaaag gcgggttggt ctctccagag aaagttaccg ccatcgttaa cgccactgga
     1141 gggcagatca aggcttacga aattcgcccc gatttgccac acctgtttcc aaaaccgaat
     1201 caggcagcat aagtaacacc gctctttaac agtcatggtc ctcattcccg ccgaaatgcg
     1261 ggaatacaac gcgcataagt tgatgcgcat aacttcttat ttgttaagga aatacttaca
     1321 tatggaactt acaagtactc gcaagaaagc gaatgcaatt acaagcaaca tcctgaatcg
     1381 aattgctgta cgtggtcagc gaaaggttgc cgacgcgtta gggattaatg aatcgcaaat
     1441 ttcgcgatgg aaagacagct tcatcccaaa aatgggaatg cttctggctg ttcttgaatg
     1501 gggtgttgaa gacgaggagt tggcggaact ggctaagaaa gtagccagaa tgctgacaaa
     1561 agaaaaagcc ccgaagaacg gcgaattctt cgaggcctga tgtagaaaga ctggatcaat
     1621 ccacaggagt aattatgaca aaacgtcgta agaaatacca ggaaaaagaa gagattcgac
     1681 accctgattc acctgagtta ttagtggtag ccgcagcaaa taacagggcg ttcgcatagc
     1741 gccttgttgg tgtttacaga ctagccaaag caggagtgaa acatgggcgt cgttaagtta
     1801 gctgattaca ggcataaccc tgtacaacat caggagacat ccagtatggg gtatgtctct
     1861 atacaccgcc agtttatgga cagcaggctc tataaggact ctcaggcagt acatctttgg
     1921 cttcacttaa tcctcaaggc taatcacgaa tctactgtcg tcaatacgga tatcggtccg
     1981 ataactgttg atcgcggtca gatgataact ggacgcccgt cgctggtcag agaaacattc
     2041 atccccgaca acaaagttcg gagcttatta cggacttttg agtcgaaagg tatgcttaat
     2101 atttgctcga tggggaagaa atttagcctg tttacaatcg ttaaatatga cgattttcag
     2161 gcaaaaaatt gtccaacggt tgtccaacgg ttgtccaacg caaacaccag taatggcgcg
     2221 gctctcagcg gagattgtcc aacggttgtc caacggttgt ccataaacaa taatataaat
     2281 aatatctcta atactgacgt attagagagt gccacagcag acaaaaagtc tgacaagaaa
     2341 aaaccttccg tcagctgtca ggatgttgtc gatgcttacc acgaaatcct tcctgaagcg
     2401 ccaagaatcc gcgcactgaa tgacaagcgt aaaaaccaga tccgaacgtt ctggcgcaaa
     2461 gccggagtaa taacacgcca acttgacggg catgggttca cgatgcagga ctggagaaat
     2521 tatttgagct acgttggcga aaattgccga tggatgttcg aagagcgccc aaaccatcaa
     2581 cgcggaaccg tctggcacaa aaagggattt gatttcctgc ttaacgataa tacctacctg
     2641 aaagttcgtg agggtgaaca cgatgaccga taatttttat gcgccgcccc atagcatcga
     2701 ggcagagcag gcggtgattg gtggattgct tctggatgat gacagcagtg agcgcgtcca
     2761 gaaagttctg gcgatgctga agcctgattc attttacagc cgaccacaca aaatcctttt
     2821 cgaagaaata accagaatgc accgggagca aaagccagta gatggcctga cgcttttcga
     2881 tgaactggag cgtaaatcgt taacggcgtc tgttggcggt tttgcttata tcgctgagat
     2941 cgcaaagaac acgccaagcg cagcaaacat cgttgcctat gcaatgcagg ttcgtgaaac
     3001 cgcaatggaa cgctacgcca tcaaccgcat gactgaagcg acggaattgc tctattcccg
     3061 caacggaatg actgcaacgc agaagtacga agctattcag gcgattttca cgcaactgac
     3121 agaccatgca aaaaccggat cgcgtcgcgg ccttcgctca tttggtgagg tcatggaaga
     3181 ctgggttagc gaccttgaga agcgatttga cccgtcaggc gaacaacgag gaatgagcac
     3241 agggatccca tcgctggaca ggatgctgtc accgaaaggt ctggtgaaag gctctctgtt
     3301 tgtcattggc gctcgcccta agatggggaa aacgacgcta tacagccaga tggcaatcaa
     3361 ctgcgcagtg catgagaaaa agcccgctct gatgttcagc cttgaaatgc caggtgatca
     3421 gatactggaa aaactggtag ggcagaagtc tggtgttaac ccgaatattt tttaccttcc
     3481 ggcgacaaat gacgccgatg acggctatca gggtgattac gatggtgact tcaacagggc
     3541 gatcgaaaca gccaatcgct tgagtgaaat cgacatgctt tacatcgacg acacgccggg
     3601 attatctctg gctcaaatcg tcagcgaaag ccgtcgaatc aagcgagaaa aaggatgtgt
     3661 tggcatgatt ctggtcgatt acctgacact aatgaccgct gagaaggccg atcgcaacga
     3721 ccttgcttac ggcatgatca ccaaaggact gaagaacctt gccaaagagc ttgattgcgt
     3781 tgttgtgctt ctgacacagc ttaaccgcgc actggaaagc cgaaccaata aacgcccatt
     3841 accaagtgac tcacgagata cagggcagat tgaacaggat tgcgattatt gggtggggat
     3901 ccatcgtgaa ggtgcttttg atgacagtgt tccacctggt gaaaccgaac taatccttcg
     3961 tctcaatcgt catggcaata ccggcacggt gtattgcatt caggcaaatg gcgctattta
     4021 tgacacagac caacagtctg ctgaaatgcg ccgacgtgaa cgcgaggaac cgcagtccaa
     4081 gaagaaagga ggattctgat gaccatctac atcactgagc taataacagg cctgctggta
     4141 atcgcaggcc tttttatttg ggggagagta aatcgtggtt gagtttatgc tcgtcgcact
     4201 caaatgcgtt ggcgttggat ggattcttct gacgtttttt attgttctgc atagctacat
     4261 tcgtcttgtg aatgacggta aagacccatg gtatacgttg tttggcgctg catttgtctg
     4321 ggtgattatc ggtgttatgc ctgtcgctgt agcaaaaatg gcgtggcgtt ttgtgagttg
     4381 aactgagggt aagtatcgat ggacgaatca agaaagcagt ttgaggaata cgttgccaaa
     4441 aaattgagat taccattcga gatgataacc gaggcaagaa atggtgatag gtacttcgca
     4501 ttttcaagta tggatattcg tcactcctta aatgagtggt ggactttatg gcaggcatcg
     4561 cgagcagcta ttgaaataac cgcgccaaag tttatcgaca gcagagaagc attagccaaa
     4621 gggtttactg ttgattattc caatggattc ggtgatggaa tggatgctta tgaggaaaac
     4681 atccgcgctg ctggaatcaa agtgaaggag tgagcatgag tcgacgaagt agctttttgg
     4741 ggtttgtaat attcctgtcc tgcactggtt acatcgtaat ctggtcaatt tcgaacattg
     4801 accgtggcgg ggaatatctc attgtaatgt tctttccttt gtttcttggg tggtacgccg
     4861 caaggttgct ggaagaatgg ggttacaggc ataaaaaata aaggagtatt cagtgaagca
     4921 aacaatcttc ctccgaacta agcaacaaca gcaagccgca atcaacgcca tcctcgcaac
     4981 tcctctcgat aaagacaaac cagtcaccat ccgcattact gactacaagc gcaaccttga
     5041 ccagaacgca aaatttcacg cgatggtcgc agatatcgct aggcaagttc agtggcgcga
     5101 taaatggtta aaaccagaac aatggaaggt tttgttgatc agcggtcatg cagtggcaac
     5161 aaaacaggaa gctgatgttt tgcccgggct tgaaggcgaa tacgtcaaca ttcgcgaaag
     5221 tagcgcgcag atgagtgtga agcgtatggc aagtctgatt gagtacacga cagcatgggc
     5281 tattggtcag ggtgtcagat ttaccgacag gaggtacgaa tgagacgaca gcgacgaagt
     5341 ttcaccgaca tcatctgcga aaactgcaaa taccttccaa cgaaacgctc cagaaataaa
     5401 cgcaagccaa tcccaaaaga atctgacgta aaaaccttca attacacagc tcacctgtgg
     5461 gatatccggt ggctaagata tcgtgcgagg aaatgacaat ggattattca cagttaagtg
     5521 attttgaaat taacagaatg gtaggagaca taatttttaa aggcctttgg gcaagtaaac
     5581 cggaaacatc agggaataac accaacaaat ggtattacgg aaatgctgat acaacttttg
     5641 agccattaaa tcatttgcct gactactgca atgatccgag cgcttcatgg ccgattattg
     5701 agaaatacag gatttctatc ttagaccagt taactgaatg gtgtgtggat gcaaaaggcg
     5761 taagcccaat atttgatgcc agacctctcc gcgccgccat gattgtcttt ctcctgatgc
     5821 aggaggccaa taatgcttag cccatctcaa tcccttcaat acctgaaagg aagcatagag
     5881 cgggcttcaa tgtgcacaga gtggattcta tctaggttta gcgcatacag aagattgccg
     5941 gtaaagggca tgccaagcaa gtcgatgctg catatgcaaa agaatgcgcg ctggaaggta
     6001 tggcgagaac acaggttatc tggctgaaag agggggttat taaggcgtga atacctacag
     6061 catcacatta ccctggcctc cgagcaataa tcgctattac cgccataatc gcgggcgcac
     6121 gcacgttagc gcagaggggc aggcatactg cgataacgtc gcccgaatca ttaaaaacgc
     6181 aatgctggat atcggcctgg ctatgcctgt gaaaatccgc attgagtgcc acatgccgga
     6241 tcgccgtcgc cgtgacctgg ataatctgca aaaagcctct tttgacgcac tcaccaaagc
     6301 aggtttctgg ctggatgatg ctcaggtcgt tgattaccgc gttgtgaaga tgcctgttac
     6361 caaaggtggg aggctggaac tgaccatcac cgaaatgggg aatgaatgat gtttgagttt
     6421 aatatggcag aacttcttcg ccaccgctgg gggcgtctgc gcttatatcg tttccccggt
     6481 tctgttttga ccgattaccg aatactgaag aattacgcca aaaccctgac aggagcagga
     6541 gtatgaagtc agagataaca atcaactaat actgttttgt tgatttttgc ttgtaattgg
     6601 cgttctggtc tgatttttgt ggagtaagtt gatgcgtgat attcagatgg ttcttgagcg
     6661 ttggggagcg tgggcggcta ataatcatga agatgtgacc tggtcgtcca ttgccgccgg
     6721 ttttaaggga ttaattactt caaaagtaaa atctcgcccg caatgttgtg acgatgacgc
     6781 gatgatcatt tgcgggtgca tggcccgtct gaaaaagaac aacagcgatt tgcacgattt
     6841 attagtagat tattatgtag tcggtatgac attcatgtca ctggcaggta agcattgctg
     6901 ctctgatggt tatatcggga aaaggttaca gaaggctgag ggtataattg aagggatgtt
     6961 aatggcatta gatatccggt tagagatgga tatcgttgtt aataactcta attaatatgc
     7021 caattgttt
//
LOCUS       DAPHCV010000173         6951 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.175, whole genome shotgun sequence.
ACCESSION   DAPHCV010000173 DAPHCV010000000
VERSION     DAPHCV010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.175"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            487..1476
                     /gene="araF"
                     /locus_tag="RH033_004330"
     CDS             487..1476
                     /gene="araF"
                     /locus_tag="RH033_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HDV2993421.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1546..3060
                     /gene="araG"
                     /locus_tag="RH033_004331"
     CDS             1546..3060
                     /gene="araG"
                     /locus_tag="RH033_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HDV2993422.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRTHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     ADQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3075..4061
                     /gene="araH"
                     /locus_tag="RH033_004332"
     CDS             3075..4061
                     /gene="araH"
                     /locus_tag="RH033_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HDV2993423.1"
                     /translation="MSSVSTSGSGAPKSSFSLGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLINGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4228..5028
                     /gene="otsB"
                     /locus_tag="RH033_004333"
     CDS             4228..5028
                     /gene="otsB"
                     /locus_tag="RH033_004333"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HDV2993424.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDTILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTAIAQFPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            5003..6427
                     /gene="otsA"
                     /locus_tag="RH033_004334"
     CDS             5003..6427
                     /gene="otsA"
                     /locus_tag="RH033_004334"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HDV2993425.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNKFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTACGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6434..6862)
                     /gene="uspC"
                     /locus_tag="RH033_004335"
     CDS             complement(6434..6862)
                     /gene="uspC"
                     /locus_tag="RH033_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HDV2993426.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKYH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1736 a         1646 c         1815 g         1754 t
ORIGIN      
        1 ttttttcatt tttgcgagat ggctctcatt atacgtgttc tgttaattaa tgagcacagt
       61 gataattaat ggcgaaatga atgacgcaca ttttccacat ctttgagttg tggttattac
      121 accattgcaa aaactcaacg ccaggtaatg cggcctattg actggttaaa aagaagacat
      181 cccgcatggg taccaaagac aacaaggact tccaggctaa tcttatggat tattctgctg
      241 tgcactcgat aatttgtctg acaaattcgc ttttccttat gtcttttccc gctaaattta
      301 tgcacgttct cactgtaatt ctgcgatgtg atattgctct cctatggaga attaatttct
      361 cgctaaaact atgtcaacac agtcacttat cttttagtta aaaggtaatg ctttgttttc
      421 cgattaattt aacgaatgtc attcgttttt gccctacaca aaacgacact aaagctggag
      481 agaaccatgc acaaatttac taaagccctg gcagccattg gtctggcagc cgttatgtca
      541 caatccgcta tggcggagaa cctgaagctc ggtttcctgg tgaagcaacc ggaagagccg
      601 tggttccaga ccgaatggaa gtttgccgat aaagccggga aagatttagg gtttgaagtt
      661 attaagattg ccgtgccgga tggcgaaaaa acattgaacg ctatcgacag cctggctgcc
      721 agtggcgcga aaggtttcgt tatttgtact ccggacccga aactcggctc tgccatcgtg
      781 gcgaaagcgc gtggctacga tatgaaagtc attgccgtgg atgaccagtt tgttaacgcc
      841 aaaggtaagc caatggatac cgttccgctg gtgatgatgg ctgcgaccaa aattggcgaa
      901 cgtcagggcc aggaactgta taaagagatg cagaaacgtg gctgggatgt caaagaaagc
      961 gcggtgatgg cgattaccgc caacgaactg gataccgccc gccgccgtac aacgggttct
     1021 atggacgcac tgaaagctgc aggattcccg gaaaaacaaa tttatcaggt acctaccaaa
     1081 tctaacgaca tcccgggggc atttgacgct gccaactcaa tgctggttca acatccggaa
     1141 gttaaacatt ggctgatcgt cggtatgaac gacagcaccg tgctgggcgg cgtacgcgcg
     1201 acggaaggtc agggctttaa agcggccgat atcatcggta tcggtattaa cggtgtggat
     1261 gcggtgagcg aactgtctaa ggcacaggca accggcttct acggttccct gctgccaagc
     1321 ccggacgtac atggttataa gtccagcgaa atgctttaca actgggtagc aaaagacgtt
     1381 gaaccgccaa aatttaccga agttaccgac gtggtgctga tcacgcgtga caactttaaa
     1441 gaagaactgg agaaaaaagg tttaggcggt aagtaatttg ccggaaagat tcccctctgc
     1501 atgatgcaga gggggtgtga acgaccagtg attcacggag acgttatgca acagtctacc
     1561 ccgtatctct catttcgcgg catcggtaaa acgtttcccg gcgttaaggc gctgacggat
     1621 attagttttg actgctatgc cggtcaggtt catgcgttga tgggtgaaaa tggcgcagga
     1681 aaatcaactc tcttaaaaat cctcagcgga aactatgcgc caaccacggg ttctgtagtg
     1741 attaatgggc aggaaatgtc cttttccgac acgaccgcag cacttaacgc gggggtggcg
     1801 attatttacc aggagctgca tctcgtgccg gaaatgaccg tcgcggaaaa catctatctc
     1861 ggccagctgc cgcataaagg cggcattgtc aatcgctcat tgctgaatta tgaggcgggt
     1921 ttacaactta aacatcttgg tatggatatt gacccggaca cgccgctgaa atatctctcc
     1981 attggtcagt ggcagatggt tgaaattgcc aaagccctgg cgcgtaatgc caaaattatc
     2041 gcctttgatg agccaaccag ctccctctct gcccgtgaaa tcgacaatct tttccgcgtt
     2101 attcgtgaac tgcgaaaaga ggggcgggtg atcttatacg tttctcaccg tatggaagaa
     2161 atatttgccc tcagcgatgc catcactgtc tttaaagatg gacgttatgt caaaaccttt
     2221 accgatatgc agcaggttga ccacgacgcc ctggtgcagg cgatggtcgg gcgcgacatc
     2281 ggcgatatct acggctggca accgcgtagt tatggcgagg agcggctgcg tcttgatgct
     2341 gtgaaagcac caggcgtacg tacgccaata agtctggcgg ttcgcagtgg tgaaattgtc
     2401 ggtctgtttg gtctggtcgg ggcagggcgt agcgaattaa tgaaaggcat gtttggcggg
     2461 acgcaaatca ccgccggtca ggtttatatc gaccaacagc cgatcgatat tcgtaaaccg
     2521 agccacgcca ttgccgcagg catgatgctc tgcccggaag atcgcaaagc ggaaggcatt
     2581 attcccgtgc actccgttcg cgacaatatc aacatcagtg ccagacgtac acatgtgctc
     2641 ggcggttgtg taatcaacaa cggttgggaa gaaaacaatg ccgatcacca cattcgttcg
     2701 ctcaacatca aaacgccggg cgctgatcaa ctgatcatga atctctcagg cggaaatcag
     2761 caaaaagcca ttctgggccg ctggttatcg gaagagatga aggtcatttt gctggatgaa
     2821 cctacgcgcg gcattgatgt tggtgctaag cacgaaatat ataacgtaat ttatgcgctg
     2881 gcggcgcagg gtgtggcggt gctgtttgcc tccagcgact tacctgaagt cctcggcgtt
     2941 gccgaccgga ttgtggtgat gcgggaaggc gaaatcgccg gtgaattgtt acacgagcag
     3001 gcagatgagc gtcaggcact gagccttgcg atgcctaaag tcagccaggc agttgcctga
     3061 gtaaggagag tatgatgtct tctgtttcta catcgggttc tggcgcacct aagtcgtcat
     3121 tcagcttagg gcgtatctgg gatcagtacg gcatgctggt ggtgtttgcg gtccttttta
     3181 tcgcctgtgc catttttgtc ccgaactttg ccacctttat taatatgaaa gggttgggtc
     3241 tggcaatttc catgtcgggg atggtggctt gtggcatgtt gttctgcctt gcttccggtg
     3301 actttgacct ttctgtcgcc tccgtaattg cctgtgcggg tgtcaccacg gcggtggtta
     3361 ttaacctgac tgaaagcctg tggattggcg tggcagcggg tttgttgctg ggcgttctct
     3421 gtggcctgat caatggcttt gttattgcca aactgaaaat aaatgccctg atcacgacac
     3481 tggcaacgat gcagattgtt cgaggtctgg cgtacatcat ttcagacggt aaagcggtcg
     3541 gtatcgaaga tgaaagcttc tttgcccttg gttacgccaa ctggttcggt ctgcctgcgc
     3601 caatctggct caccgtcgcg tgtctgatca tctttggctt gctgctgaat aaaaccacct
     3661 ttgggcgtaa taccctggcg attggcggga acgaagaggc cgcgcgtctg gcgggtgtac
     3721 cggttgttcg caccaaaatt attatctttg ttctctctgg cctggtatca gcgatagccg
     3781 gaattattct ggcttcacgt atgaccagtg ggcagccaat gacgtcgatt ggctatgagc
     3841 tgattgtgat ctccgcctgc gttttaggtg gcgtttctct gaaaggcggc atcggaaaaa
     3901 tctcatatgt ggtggcgggt atcttaattt taggcaccgt ggaaaacgcc atgaacctgc
     3961 ttaatatttc tcctttcgca cagtacgtag ttcgcggctt aatcctgctg gcagcggtga
     4021 ttttcgaccg ttacaagcaa aaagcgaaac gcactgtctg atgctttttt ccacaacaat
     4081 ttagcgtttt ttcccaccac agccagccgc cacaacggtt ggctgttctt cgttgcaaat
     4141 ggcgaccccc gtcacactgt ctatacttac atgtctgtaa agcgcgttct gcgcaacaca
     4201 ataagaaaag agaaggagga gaaccgggtg acagaaccgt taaccgaaac ccctgaacta
     4261 tccgcgaaat atgcctggtt ttttgatctc gatggaacgc tggcggaaat caaaccgcat
     4321 cccgatcagg tcgtcgtgcc tgacactatt ctgcaaggac ttcagctact ggcaaccgca
     4381 agtgatggtg cattggcatt gatttcaggg cgctcaatgg tggagcttga cgcactggca
     4441 aaaccttatc gcttcccgtt agcgggcgtg catggggcgg agcgccgtga catcaatggt
     4501 aaaacgcata tcgttcatct gccggatgcg attgcgcgtg atattagcgt gcaactgcat
     4561 acggctatcg cccagtttcc cggtgcggag ctggagacga aagggatggc ctttgcgctg
     4621 cattatcgtc aggctccgca gcatgaagac gcattaatga cattagcgca acgtattact
     4681 cagatctggc cacaaatggc gttacagcag ggaaagtgtg ttgtcgagat caaaccgcga
     4741 ggcaccagta aaggtgaggc gattgctgcc tttatgcagg aagctccctt tatcgggcga
     4801 acgcctgtat ttctgggcga tgatttaacc gatgaatctg gcttcgcagt cgttaaccgg
     4861 ctgggcggaa tgtcagtaaa aattggcaca ggcgcaactc aggcatcatg gcgactggcg
     4921 ggcgtgccgg atgtctggag ctggcttgaa atgataacca ccgcattaca acaaaaaaga
     4981 gaaaataaca ggagtgatga ctatgagtcg tttagtcgta gtatctaacc ggattgcacc
     5041 accagacgag cacgccgcca gtgccggtgg ccttgccgtt ggcatactgg gggcactgaa
     5101 agccgcaggt ggactgtggt ttggctggag cggtgaaaca gggaatgagg atcagccgct
     5161 aaaaaaggtg aaaaagggta acattacgtg ggcctctttt aacctcagcg aacaggacct
     5221 tgacgaatac tacaacaaat tctccaatgc cgttctctgg cccgcttttc attatcggct
     5281 cgatctggtg caatttcagc gtcctgcctg ggatggctat ctacgcgtaa atgcgttgct
     5341 ggcagataaa ttactgccgc tgttgcaaga cgatgacatt atctggatcc acgattatca
     5401 cctgttgccc tttgcgcatg aattacgcaa acggggagtg aataatcgca ttggtttctt
     5461 tctgcatatt cctttcccga cgccggaaat cttcaacgcg ctgccgacat atgacacctt
     5521 gcttgaacag ctttgtgatt atgatttgct gggtttccag acggagaacg atcgtctggc
     5581 gttcctggat tgcctttcta acctgacccg cgtcacgaca cgtagcgcaa aaagccatac
     5641 ggcctgcggc aaagcgtttc gaacggaagt ttacccgatc ggcattgaac cgaaagaaat
     5701 agcgaaacag gctgccggac cactgccgcc aaaactggcg caacttaaag cggaactgaa
     5761 aaacgtacaa aatatctttt ctgtcgaacg gctggattat tccaaaggtt tgccggagcg
     5821 ttttctcgcc tatgaagcgt tgctggaaaa atatccgcag catcatggta aaattcgtta
     5881 tacccagatt gcaccaacgt cgcgtggtga tgtgcaagcc tatcaggata ttcgtcatca
     5941 gctcgaaaat gaagctggac gaattaatgg taaatacggg caattaggct ggacgccgct
     6001 ttattatttg aatcagcatt ttgaccgtaa attactgatg aaaatattcc gctactctga
     6061 cgtgggctta gtgacgccac tgcgtgacgg gatgaacctg gtggcaaaag agtatgttgc
     6121 cgctcaggac ccagccaacc cgggcgtact tgttctttcg caatttgcgg gagcagcaaa
     6181 cgagttaacg tcggcgttaa ttgttaatcc ctacgatcgt gacgaagttg cagctgcgct
     6241 ggatcgtgca ttgactatgt cgctggcgga acgtatttcc cgtcatgcag aaatgctgga
     6301 cgttatcgtg aaaaacgata ttaaccactg gcaggagtgc ttcattagcg acctaaagca
     6361 gatagttccg cgaagcgcgg aaagccagca gcgcgataaa gttgctacct ttcctaagct
     6421 tgcgtaggag cggttaatct cccgtaagtg gaactaacag cacatcgacc tcacttgagg
     6481 caatcactct tttcgccgag caggatgctc gcgaaaagaa actgtgattg tgattaccgc
     6541 aaatcaccaa atcgaaatga tatttgcgac aaacttccag aatatgttcg cttaactcac
     6601 cgtaggcaat aaacgtcttg tcaacgggat accccgcatc ctgaattaac ttatcaagaa
     6661 agctttgcgt ttcttcctgc atcacgctgc gtaaatcttc aagcatcggc gcagctaatt
     6721 gattgtacat ttccgggtcg gaagcgagag taattaaact gatgtgtccc tttactggcc
     6781 tggcgataga taccgctttt gccagcagtt gctgactttc cggtgtaacc gcaacagcga
     6841 caagaatatt gctatagctc ataacctgtt ccttattctg tgaacttcag gtgacattaa
     6901 agctttactc ctaatggaga aacgacgcaa tcccaactca gtcaaacagg t
//
LOCUS       DAPHCV010000174         6947 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.176, whole genome shotgun sequence.
ACCESSION   DAPHCV010000174 DAPHCV010000000
VERSION     DAPHCV010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.176"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..2771)
                     /locus_tag="RH033_004336"
     CDS             complement(<1..2771)
                     /locus_tag="RH033_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDV2993427.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNEVAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIR
                     SKETLAENMTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSKINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGDVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRINIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTTVKQYLS
                     HPVNTYTGV"
     gene            complement(2828..5200)
                     /locus_tag="RH033_004337"
     CDS             complement(2828..5200)
                     /locus_tag="RH033_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDV2993428.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSSVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDFTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDISGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(5238..6923)
                     /locus_tag="RH033_004338"
     CDS             complement(5238..6923)
                     /locus_tag="RH033_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HDV2993429.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLSVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVI"
BASE COUNT         1792 a         1602 c         1559 g         1994 t
ORIGIN      
        1 aatactccgg tataagtatt taccggatga gaaagatatt gtttaacggt agtgttaaca
       61 ttctctaccg tcatttgttt caacaattgc tcctgctcag tccatgctgc aggatcgtca
      121 tattgaataa gactatttac aatggtgttc gctaattgtt gaacgctacg ctgttggata
      181 tcgaggctgc gctgaacgtt ttgctggtat tcgttcagtt cttgctcact gatcccttta
      241 gccagacgct taaccatcac ttcattcgct aacgttaaca gttcatcatg tcgttctggt
      301 tgacaagtaa aagccagcaa atgactgata tctttggcct gaggatcaac cgagaggcga
      361 gaagaaacgc tgtatgcccc agaggcctgt tcacgaatat taatgcgtag atcttttgcc
      421 agtgcgacgt taaaagcatc gagcgccata cgcgtcgcca gattaaccgg tgtccgggaa
      481 tcataacgct tccactgtga aacctgtgcc acaggttcat tttgttcttt taccgtaacc
      541 gatgcattgt ccgtcgcgcg agttaatggt ttacctgcgg ctaatggcga atcagagtgt
      601 ttgattgatc ctaagtaacg cgtaattaac gccacgagtt tgtcttctgc gacatcaccg
      661 acaatgacaa acgtgatatc cgctggagat gaaaacaact ggcgatcggc agccagcgca
      721 tctgcggcag taaactgtgc aatctgattt tcttgcagta atttcgtgcg gtcatcagca
      781 taacgcgtct catacatctg ctgggcgaat ttctccgctg gacgctggtc gagcgttttc
      841 aacgcctgaa tttgagcatt ttgtagcgat gcccaaatat tgtcgttaat cttgctgtgg
      901 gtgattcgct ggttaatcaa ctggaaacca ggttcagggt tattagtccg cgcgctaaca
      961 gagagcaatg tattcatgcc actgacttta ctgctcatgg ttaccgagtt ttccgcactc
     1021 cagcgtttca ggctggaaga ggagagttca ccaacgccgc ttccgcttac tgctttattt
     1081 gccagcgcga taagtgattt ttgctgcgca gggaaactta aatcgccttt attcgatacg
     1141 gcagtaattt gtagcttttg ctcttcaccc gcggattttg ccagaataac cctggcacca
     1201 ttagaaagtg ttaatgatgt catattctca gccagcgttt ctttgcttct aatttccgcc
     1261 tgagggtcag cgtctactgt taacgataaa tttctgcctg ggaagatgta agccgccagc
     1321 tttttgttgg cgtactcttt ttccagcgcc aggatagccg caggagacaa tgcttttttg
     1381 gcggcaactt cattgtttac catttgctcc caaaatgcgt cctggttctt tcttaactgc
     1441 tgccattttt ccgccagact ttgcacggta atttgctgcc acagacgttt cgaaagttga
     1501 tatgtctctt ccggcgacaa gaacggcgta ttatttaatg agctggatgc caggcgactg
     1561 gtcagcatac gtaaatcacg ttcagcttgc tgatcaaccg catttttcag ccaggtgagg
     1621 cgggtagatt tgacatcatc gagttcttca gcagaaaaac catgctgatc aatggttgcc
     1681 aactctgcca ttaatgcatt cgcagcatcc tgcatattat cgtctcgtgc atttacacgg
     1741 aaaaacagcg actgataatc gggtgcaatt ttgacgctgc gcgcagtgcc gccagagata
     1801 gtcttcaact cgcccgactg tatgcgttcc tgcagacgtt ggttgaacag ctgaaccaac
     1861 atgctccatt cagcttgttc gataaagctt tgctcatcgt tcacttgtac cattggcagg
     1921 cgataataga gtgcgatgcc gttcacccgg ttttctttat cattgatgat attaaagcgc
     1981 aggtggtttt cggcttttgt cggccagacg cgattttcag ctgctttgtt agccggaagc
     2041 ttacttaaat tatcctttat cagcgccagc gcttctttac tgtcgatatc gccgaccaca
     2101 ataaaggtca tattatttgg ttgataccag cgttgataaa attggcgcaa ttgtgccggt
     2161 gtgaccgtgg cgacggtatc catcaggccg ataggttcac ggtctaaatt acgggtattt
     2221 gccagcagga aagggcggcg cgcctgagag gtgcgccatt tcgcatcctg atgggcacgc
     2281 cattcctcag taattacgcc gcgttcagcg tctacttcga gtttttcaaa ggttgcggca
     2341 ttactccatt cactgaagat tgccatcact tgttgcagat tttgtttctg cgtagtcggc
     2401 aaactcacct gatacactgt ttcgtcatag ctggtatagg cattaacatc gcgaccaaaa
     2461 cgcaggccca ttgactcaaa tgtttcgatg actttattac ccggccatgt ttttgtgccg
     2521 ttaaacatca tatgttctac aaaatgagcc acgccgcgct cattgtcttc ttcctgcaat
     2581 gaaccggtat gaatttgcag ccataaattt acctgatcct ttggttgagc atgcggataa
     2641 atcatatatc gcaagccatt gtccagttgc ccggtaatta acttttcatc ctgcggtaag
     2701 gcggcggcaa tcagtcgccc ggggagcaac agagttgcca ctaacgtcag taagaaacag
     2761 aggtttctca taattatctc catgcgaaaa ccgggcaaat ttacccggtt aagtaaaatc
     2821 cgaactatta aaatttcatg ctgacatcaa gccagaaagt acggccactg gcgtaggtcg
     2881 ccacaccggt attcgttgta tcaaccgcgg ttttgctatc cagtacattg agaatatcgg
     2941 cactgatcgt caggttttgt tgtttcagaa attgtggcgt ccaggacaag cgggtgtccc
     3001 aggtcaagct gctatccagt ttttcgtcaa catactgctt gtaatctgaa tattcgctga
     3061 tgtattgcgc attcgtctta cccaggataa tgcgagcttt acgcgcttct tgccaggcca
     3121 gcgtattcgc ccacaccaac ccgctcggtt gatgcgtgaa atccatgttt aaggagatct
     3181 ttaatgggtt attaaaatct gccactggaa cgctatcgta agagaccaga ttaccgttat
     3241 aaaccacctg gttatcaccg gtattgcttt cctcataacc attgtttaac gacaagttgc
     3301 ccttgctctt gatatagcta aagacaattt gcgggttaat atctacctgg ctgatatgca
     3361 ggggttcggc cagttcaaaa ctgaggttga acgaatgcgt tttagttttg ccgtcgttgt
     3421 tatattcagt aatggtggtt ttagtcgcgc tgtcggtacg actgctttta ctgatttgat
     3481 catgcgcttc acgataaaca tagtttgcac gcgcaataac gttcttaccg attttctgct
     3541 gcaaccccat tgccagttca tcgttataag gcgttttcaa atcctgataa cgcgtcaggg
     3601 ttttattgcc tgataccgat tccgtccagc tattgcggat atcacgtaat cccatatcaa
     3661 gaatattccc gccatagtaa cggttataac cggcggtaat cattgaggtt tgatcagcaa
     3721 aaatatccca ttccgtcata aatcgcgggg agatattgtg gtttgacaga tagttgtcat
     3781 agtcataccg cacaccgggc attaacgaca cattccgcca gctaatgcga tccgccatat
     3841 acagcgtgta gttgtcaatt cccagactgc ctttaccttt atggtaaatg gtatggttag
     3901 ttttctttcc tgcagcatta atcacatagg attcagactg gttatggcgt tcagtccatg
     3961 catcggaata gatgtattcc gcgccgaagt agggttgatg cgaaacatca cccacggcga
     4021 atttttgcca gtccaggcgt gttttgaagg tgtaattatc tacagcctgg gaaatgtgtc
     4081 ctaatccacc acgggtgcaa cgacctgaaa tatcaccata tgtacatgaa agttcggtgt
     4141 accagatgtc atgatcgtga cgggtataat cactaatatg atcccaacca acggtagtac
     4201 gtagtttggc ccatgcgagc tgcgtatcca tatcccatgc cagaccgtag gatttattac
     4261 ccatttcgcg atcagactgc gggaaggtgc tggtattata atcacgactg gagccggtat
     4321 attttaaggt aaaatcgtgg gtaaagcggt cgctggcaaa ccaagtaaat ttgctcaatg
     4381 cggtatcgat aacgttttta tactgtgccc gaccggcgac aatgccgtca ttcgaaacat
     4441 aatccgcgcg ggtgatatca gactggcggc gcgataaacc ggcggtaacg ccaaagttat
     4501 cagcgagttc ctgattaaac gacaaggtat aaaagttctt tttaaaatct ggagagtaat
     4561 aagtacttcc tgaagaacct tgattaaatg cgctcttgtt attctcatcg atatgcgatg
     4621 ttaaccagtc cgaacgcgta gtgcgataac ccagtttcac gctgctatca tcagcgttga
     4681 agcgtttgat ctttgcatca attaccccgc cattgaagcg accgaattca accggcacaa
     4741 aactgtcata aagcgtcaca ttgtccagta agctgacatc aagataatag ccctgtgaca
     4801 tcccgctaat attggttgca ctactggcat cggactcatt cgctgggttc aggttattag
     4861 ttgcactaat accatcaatc aaataggcat tctggtaggg cgacgcaccg tgaatagaga
     4921 ttttctccgg gcgaatatct ccctggttca atgaggtact ttgcgttgaa tccatgcgca
     4981 ccgcagggtt ggttctcagc agatcgctga tattgccatt cccggtcggt aaacgctcga
     5041 tgctttcact ggtgtaatgc gtgttgccgt ttacgggtac agggacagga gcatagaccg
     5101 tcatctcttc aaaaacatgc atgtacatgt ttttgtcatc gacggcctgc gctacatcag
     5161 cgccacataa aatgacgcca ggaataagaa ctcgcttcat ttatttatat ctccgtacag
     5221 cagggctttt attgttttta tataaccgcg ctaatgtcac aaatatcatc agccaggttc
     5281 cagacaccgg gttgatgtgt aaccataatg acgccgcttg tgggtagttt ttcacgcact
     5341 aaacgcagta agcggatagc ctcttgttcc tcaagatgag aggtagtttc gtcaagaaat
     5401 atccatttcg gacgtcgtaa aattaatcgt gccagggcga tacgttgttt ttcgccgctg
     5461 gaaagaatat cgccccagcg atcgtggtca tgaatacgcg cagccaattt ccccagacca
     5521 acctgatgca gtacttcgct caacgattta tcgtctacgg acaggggaag tgctttacaa
     5581 attatctctt tcagtaagcc ggttttgatt aacggtgttt gtgacacata ccaactgtca
     5641 gcaggagaag aaatatcacc tttaaaccac ggccagcagt gggataatgt tttaagcagt
     5701 gtggtttttc ccgcaccaga gtaacctttc agtaatagcc atttgcctgg cgaaacatga
     5761 aagttcaggt tctctaatat gatcttatta tcaggagtac gaatactcgc gtcagccact
     5821 tgtaccgcat gttggcaatt tttaggctta ttcgtagggc gctgttcggt gagttgatgg
     5881 aactcataca aacgatcgat aaccgcagcc agttcagcaa gttcgtcata tttataaata
     5941 aaccagctta aattgttcga taccagcata aatgcctggc gcgatttcat cagtccgccc
     6001 agattgatct gtccgctaat aaactgcggc aataacagaa agtagggaag aacgctaagc
     6061 gagcgcgaat aaatattctg ccagtaatca agccaccgct gacgattcat taaacgatgc
     6121 cagttctctt taatggtatg aaaattgtcg cttaactcct ggcgttgtag agactcagca
     6181 ttgcttagcg caatcagttc tgcctgctta ttatgctgca caagattagt tcgaaatgtc
     6241 gcttcgctac gttgtttttc cacattaagc ggacgaatac gtttaccaac cttatgggta
     6301 aataaagttc caccgatcac aatgagcaca acggtataga ccatataccc ctggatattc
     6361 cattctgttc caccaacagt gaatgagagc gtacccgcgc tttgccaaag aataacggta
     6421 aaggtgatca acatgctaag tgactggatg aagccaaatg aaaggctgag cgttttactg
     6481 atcagtaaga gaatgtcctc agcgatacgt tggtcagggt tatctgtatt tttatgctca
     6541 ccgtagatct gcgtgaagta gtagttttta tctgcaaacc agcgattaag gtagtaatct
     6601 gttaaccatt cgcgccagcg aatagtcagt agtttaatta gccatgtttt gtttacggat
     6661 attaatacaa agatccccag cagggcaggg aaccaaagga caagctgcca aagcttatcg
     6721 gtttctttct ggcttagcgc attgaaaaaa tcattattcc agtcgtttaa ccagacctga
     6781 attttaacca cgccaaggat catggcgagg ataataatga ttaacaacac tgaagtttta
     6841 ttattcttac gcagccaaaa tggcttcagc agacagagat actttgctat caacatacaa
     6901 aacgtaatgg gaatggttat cattagcgaa aattgattgt gccatcc
//
LOCUS       DAPHCV010000175         6659 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.177, whole genome shotgun sequence.
ACCESSION   DAPHCV010000175 DAPHCV010000000
VERSION     DAPHCV010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.177"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1210
                     /locus_tag="RH033_004339"
     CDS             join(<1..297,297..1210)
                     /locus_tag="RH033_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDV2993430.1"
                     /translation="EKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAASQLFLWRK
                     QYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVEYGRGKKV
                     DSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWKDGRRSRHSDDTDVLLRIHHV
                     IGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKTAVPPSKRAHT
                     GKVAVKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTGGFDSETVQDV
                     MLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGIA
                     ESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQQASN
                     GLSDNRCLEI"
     gene            1455..1775
                     /locus_tag="RH033_004340"
     CDS             1455..1775
                     /locus_tag="RH033_004340"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDV2993431.1"
                     /translation="MQQFRHWQNRLNTIDPARIKLYSETVRVWNQSRGSAGARALADM
                     LIQNGIPMSSYRARRLMKHLNLRSAQPGKYQYKNARQEHTSLPSLLECRFSVLEAEQV
                     WILK"
     gene            complement(1894..2640)
                     /gene="istB"
                     /locus_tag="RH033_004341"
     CDS             complement(1894..2640)
                     /gene="istB"
                     /locus_tag="RH033_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family helper ATPase
                     IstB"
                     /protein_id="HDV2993432.1"
                     /translation="MNHLYEQLTALKLTGFRDALKKQLAQPGTYQELGFEERLSLLTA
                     EELTCRENRKAERLIKHARFRLNAELSKLDYRNNRGLDRALIRSLSQGNWLTLKQNIL
                     LTGATGSGKTFLACALGHNACRQGYKVYYYRLKALMEQCYQGHADGRYSKLLTRLNNS
                     DLLLLDDWGLEPLSSEQRSDLLEIVDLMYQRGSIIVVSQLPVENWYKMIGDSTHADAI
                     LDRLVHGSIKIELKGESMRKIQSPLTEGDQ"
     gene            complement(2655..4196)
                     /gene="istA"
                     /locus_tag="RH033_004342"
     CDS             complement(2655..4196)
                     /gene="istA"
                     /locus_tag="RH033_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family transposase"
                     /protein_id="HDV2993433.1"
                     /translation="MPTVPISMRKLKEILRLKYGVGLSHRQIGRSLAISPSVVSRYAN
                     RAAQLGIKQWPLPTGWDDTKLKHAFLQTQVKMKKHSLPDWATVHRELRNKCVTLQLLW
                     EEYCERNPGGFYSYNHYCRMYREWLKTTSPSMRQVHKAGEKLFVDYCGPTVGVTDPET
                     GEIRTAQVIVAVLGASSYTWAEATWSQQLEDWVMSHVRCFQWLGGVPELVVPDNLKSA
                     TSRACKYDPDVNPTYQQMLEHYNVAVLPARPRKPKDKAKAEVGVQVVERWIMARIRHE
                     IFYSLASLNQRIRELLERLNNKIMQKLGYSRAELFIQLDKPALKPLPEASYSYTLVKK
                     VRVHADYHVEIDKHYYSVPCSLLGQQLEAWISGELVRLFNQGQEVAVHPRKRTYGYST
                     RNEHMPEAHRQHATWTPERLLEWAGHIGSETHSYVLHILNSRPHPEQSYRFCLGLLNL
                     HKKYSKARLNAACARALKTKVWRLSGIKSILEKGLDKQPVQDPKPDLLSTMEHENVRG
                     SEYYH"
     gene            4796..5131
                     /locus_tag="RH033_004343"
                     /pseudo
     CDS             4796..5131
                     /locus_tag="RH033_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5181..5775
                     /locus_tag="RH033_004344"
                     /pseudo
     CDS             5181..5775
                     /locus_tag="RH033_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5972..>6659
                     /locus_tag="RH033_004345"
     CDS             5972..>6659
                     /locus_tag="RH033_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDV2993434.1"
                     /translation="MDEKKLKVLAAELAKGLKTEADLNQFSRMLTKLTVETALNAELT
                     DHLGHEKNAPKSGSNTRNGYSSKTVLCDDGEIELNTPRDRENTFEPQLVKKNQTRITG
                     MDNQILALYARGMTTREITSAFKEMYDADVSPTLISKVTDAVKEQVSEWQNRPLDALY
                     PIVYLDCIVVKVRHSGSVINKAVFLALGINTDGQKELLDMWLAENEGAKFWLNVLTEL
                     KNRGLNDILIA"
BASE COUNT         1735 a         1568 c         1676 g         1680 t
ORIGIN      
        1 cggagaaacg cagacggcgt actacacagg aaaagatcgc tattgttcag cagagttttg
       61 aaccgggaat gacggtctcc cttgttgccc ggcaacacgg tgtggcagcc agccagttat
      121 ttctctggcg caagcaatac caggagggaa gtcttactgc tgtggccgca ggagagcagg
      181 tcgttcctgc ctctgaactt gctgctgcca tgaagcagat taaagaactc cagcgcctgc
      241 tcggcaaaaa aacgatggaa aatgaactcc ttaaagaagc cgttgaatac gggcgtgcaa
      301 aaaagtggat agcgcacgcg cccttattgc ccggggatgg ggagtaagct tagtcagccg
      361 ttgtctccgg gtgtcgcgtg cgcagttgca cgtaattctc agacgaaccg atgactggaa
      421 agatggtcgc cgcagccgtc actcagatga tacggatgtg cttctccgta tacaccatgt
      481 tatcggagag ctgcccacgt atggttatcg tcgggtatgg gcgctgcttc gcagacaggc
      541 cgaacttgat ggtatgcctg cgatcaatgc caaacgtgtt taccggatca tgcgacagaa
      601 tgcgctgttg cttgagcgaa aaaccgctgt accgccatcg aaacgggcac atactggcaa
      661 agtggccgtg aaagaaagca atcaacgatg gtgctctgac gggttcgagt tccgctgtga
      721 taacggagaa aaactgcgag tcacgttcgc gctggactgc tgtgaccgtg aggcactgca
      781 ctgggcagtc actacgggcg gcttcgacag tgaaacagta caggacgtca tgctgggagc
      841 ggtggaacgc cgcttcggca acgagcttcc ggcgtctcca gtagagtggc tgacggataa
      901 tggttcatgc taccgggcta atgaaacacg gcagtttgcc cggatgttgg ggcttgaacc
      961 gaagaacacg gcggtgcgga gtccggagag taacggcata gcagagagct tcgtgaaaac
     1021 gataaagcgt gactacatca gtatcatgcc taaaccagac gggttaacgg cagcaaagaa
     1081 ccttgcagag gcgttcgagc attataacga atggcatccg catagtgcac tgggttatcg
     1141 ctcgccacgg gaatatctgc ggcagcaggc cagtaatggg ttaagtgata acaggtgtct
     1201 ggaaatatag gggcaaatcc aggtacgtca gctcaggcgg gaacgtcagg ataccacgtc
     1261 ttctgcatca ccactcacat cagagtagca acgtattcat gagctggaaa agcaggttcg
     1321 tcgcctggag gaacaaaaaa cgatattaaa aagctacagt gctcttgata tcctccccac
     1381 tgaacggttt acgatagtta ccagactgag cggcgacaca cggttgtcag tctttgcgct
     1441 gctctggatg ctcattgcag cagttccggc actggcagaa tcggctcaat acgattgatc
     1501 cggcgcgaat caaattgtat agcgaaacag tccgggtctg gaaccagagt cggggttccg
     1561 caggtgcgcg tgctctggca gatatgctga ttcaaaatgg tatcccgatg agcagttatc
     1621 gtgccaggag gctgatgaag catctgaacc tgagaagtgc tcagcccgga aaatatcagt
     1681 acaaaaatgc ccgccaggag cataccagcc tgccgagtct gcttgagtgt cggttttctg
     1741 tgctggaggc cgagcaggta tggattctta aataaacctc gagtctgaat cggtaactag
     1801 ctgttatatg cgtattaccg tgaaggagat cggtgagtaa catcgatgga gatcggttcg
     1861 tgtcactttc acagaaccgt ttttaaatta ccttcactga tctccttcgg tcaacggaga
     1921 ttgtattttc cgcattgatt ctcctttaag ttcgatcttg atactgccat gaaccagtcg
     1981 atctaggatg gcatccgcat gtgtggagtc tccgatcatt ttgtaccagt tttccaccgg
     2041 caactggctc actacgatga ttgagcctcg ttggtacatc agatccacta tttccagcag
     2101 gtcgctacgc tgttctgatg agagaggttc cagcccccag tcatccagaa gcagcagatc
     2161 gctattattc agcctggtca aaagtttgct gtatcttcca tcagcatgcc cctgatagca
     2221 ctgttccatc agcgctttaa ggcgataata gtagaccttg tatccctgtc ggcaggcatt
     2281 atgaccaagt gcacatgcca ggaacgtttt accgctgccg gtggccccgg tcagtaaaat
     2341 attttgtttc agggttaacc agtttccctg actgagtgaa cggatgaggg ccctgtccag
     2401 ccctctattg ttacgataat ccagctttga taactcagca ttaagtctga accgtgcatg
     2461 tttgatcaga cgctctgcct tcctgttttc acggcaggtt agttcttctg ctgtcagtaa
     2521 tgacaggcgt tcttcgaagc ccagctcctg gtatgtgccc ggctgagcaa gttgcttttt
     2581 aagcgcatca cggaagccgg tgagtttaag tgcggtcagt tgttcgtaaa gatgattcat
     2641 cattggatcc cgtatcagtg gtaatactca ctgccgcgta cgttttcgtg ttccatcgtg
     2701 gataacagat ctggttttgg atcctgaaca ggttgtttat ccagaccttt ttccaggatc
     2761 gatttaatac ctgacagacg ccataccttt gttttcagag ctcttgcaca tgctgcatta
     2821 agtctggctt tactgtattt tttatgaagg ttcaggagtc caaggcagaa gcgatagctt
     2881 tgttccggat gtggacgaga gttcagtata tgaagcacat aactatgagt ttcactgcct
     2941 atgtgccccg cccattccag aagacgctct ggcgtccagg tggcatgctg tcgatgagct
     3001 tcaggcatgt gctcgttgcg ggtactgtag ccataagtac gcttgcgcgg gtgcacagca
     3061 acctcctgcc cctgattgaa gagtcttacc agttctccgg agatccatgc ttccagttgc
     3121 tggcctaaca gcgaacatgg aaccgagtag taatgtttgt cgatttccac gtggtaatcg
     3181 gcatgaactc tgactttctt caccagggtg taactgtaac tggcttcagg aagaggcttc
     3241 agtgcgggtt tatcaagctg gatgaagagt tctgcacgtg aataacccaa cttctgcatt
     3301 attttgttat tcagtctttc cagcaactcc cgaatgcgct gattaagcga tgcaaggctg
     3361 tagaagatct catgcctgat tcgggccatg atccagcgtt caacaacctg aacgccaact
     3421 tcagctttgg ctttatcttt cggtttacgt ggccgcgcag gcaaaactgc gacattataa
     3481 tgctcaagca tctgctggta ggtagggtta acgtcaggat catacttaca tgccctggat
     3541 gtggcgcttt tcagattgtc cggaacaaca agttcaggaa cgccacccaa ccactggaag
     3601 cagcgaacat gactcatcac ccagtcttca agctgctgag accaggtggc ctctgcccat
     3661 gtgtaacttg atgccccgag aacagctacg atgacctgag cagttcttat ttctccggtc
     3721 tcagggtcgg taacgccaac ggtaggtcca cagtaatcaa cgaaaagttt ttcgccagct
     3781 ttatgtacct gacgcattga tggtgaagtg gttttgagcc attcacggta catccggcag
     3841 taatggttat agctgtaaaa accgcctgga ttacgctcac agtattcttc ccagagtagc
     3901 tgcagcgtca cgcatttatt acgcagttcc cggtgtactg tagcccagtc aggcagagag
     3961 tgcttcttca tcttaacctg ggtctgaagg aacgcatgtt ttagttttgt atcatcccat
     4021 cctgtaggta agggccactg ctttatgcca agttgagccg cccgattagc atatctggat
     4081 acaacggaag gggagattgc aagactacga ccaatttgtc gatggctgag tccaacaccg
     4141 tatttaagcc taagaatttc tttaagtttt ctcatagaaa ttggaactgt tggcataggt
     4201 atcctttacc ggaatggcaa aagatacaga tcaacacacc tgtgaagttc caataacatt
     4261 gatggagatc actgaataac aaaatgagtc aaaagtgatc tccatcgatg ttattcagcg
     4321 atctgttcaa atgttattac ccgatctcga tggaagttat tgagtgatct cctttcatga
     4381 aaatacgcag ttatattctg aattattaca atagcgtcag gcctcataat tacactgtgg
     4441 gttgacgccg gaagaatcag agaacagata ccgttcttac tgtaaaacgt ggctaatatt
     4501 aattgagcac tgcagtgttc agagcacaaa gaagaaaaaa gatgtgtgcg aatgcctgta
     4561 atgtgttaaa agaatgttcc gcgtttccgg tacgcttttc gtaactaaca atgcagggtg
     4621 acgtaacgct ggttgtgccg gaaacgcgtc cctcccccta tctttcctgt ttggatttgc
     4681 ccctatattt ctggattatc cgacaaaacc ggcgtgaagt attactctcg tcgcccagca
     4741 ccataaggat aacgcccata aaatcatgaa acttttcttc cagattggat acaatcaggc
     4801 cgtatcccgt attacaggct tatcacgtgt gacagtcagt ctctgggtgt gttcaggaac
     4861 attccctgaa atgtcaaccc ggcctccaga gcgaggactt ctggccccat ggagggagtg
     4921 gttaaaagag caacgagaaa gtggtaactt cggcgcgtgg ctgcaaggat ggcagctgat
     4981 gctgcggcta tatgtgaggc aatcagcagt cgctggaata atgggggttg tcgaaagtca
     5041 tgtaaatcga ctaaaaatgc tgaaaggcca gatgtatggt cgagccggat ttgaactact
     5101 caggcagagg gtcatgagtc cactggcatg aaaatctcca tccaggttgc gtaagagcca
     5161 cttttcatcc gtcgttgaca tttgtatgcc gtcactgtgg cacttctcgg cccacattgt
     5221 gtaaatggtg gcgcagatat ctggctcagg gcatcgctgg tctggaaagc tacagccgtc
     5281 gctccaagcg ttcaccatca acgaaaacca gcacttgcga agtcgctctg attctggaag
     5341 ttacgttctc agcgaaatct gggtgcaaga cgtattcaaa gcgagttaaa acgacttcat
     5401 tcaatctcac tggcaatagt gcgtattacc gtgaagggga tcggtgagta acggcgtttt
     5461 tcaaggtagt tgtcgcctga tgtaataaag gttggacgtt cccaaaagac agacaaagct
     5521 gagttttacg ctaccgtaga tctttctgct gatgacctga agggattgct agcagaatgg
     5581 tagcattact ataacaggga acgtcctcat agcgctcaca acggtaaaac tccgatggaa
     5641 cgctactttg aacttgctga gaaaacacca tactccgatg ccgttcatgc aaactatcag
     5701 cccaatgaag agcatataca ggaacagaat tacaagcctg aacttgaact gagaaaattg
     5761 aaacgatgtc tatgaatcac acattttatt acaaggcaac aactaccttc aagctctatg
     5821 ttgagcctgt ttaccattct gtgtaatatg cattttcttt aaagtgaccg tcaggacggt
     5881 cactgattac ccattctgaa gagactgtaa ttaaaattgt gtaattgcct gtttttgata
     5941 tgttcactcc aacaacggag acaggcaaac gatggacgaa aagaaactta aagtccttgc
     6001 ggctgaactg gccaaaggcc ttaaaaccga agccgacctc aaccagtttt cacgcatgtt
     6061 gacaaagctt accgtcgaaa cggcgctcaa tgcagaactg actgaccatc taggtcatga
     6121 gaaaaatgct cctaaatcag gctctaatac ccgtaatggc tactcgtcaa aaacggtatt
     6181 gtgcgatgat ggcgagatcg aacttaacac gccgcgtgac cgtgaaaata ccttcgaacc
     6241 acaactggtg aagaaaaatc agacccgcat taccggcatg gacaaccaga tcctggcgct
     6301 gtatgccaga gggatgacta cccgcgaaat tacctcagcc ttcaaagaaa tgtacgacgc
     6361 cgatgtctcg cccacgctga tatccaaagt gaccgatgcg gttaaagaac aggtctctga
     6421 atggcaaaac cgaccgttgg atgcactgta tcccattgtt tatcttgact gtattgtggt
     6481 taaggttcgt cacagcggga gtgtcattaa caaagcggtg ttcctcgcgc tgggcatcaa
     6541 taccgacggt cagaaagagc tgctggatat gtggctggcc gaaaatgaag gtgcaaagtt
     6601 ctggctgaat gtactgacgg aactgaaaaa tcgcggcctg aacgatatcc ttatcgcct
//
LOCUS       DAPHCV010000176         6526 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.178, whole genome shotgun sequence.
ACCESSION   DAPHCV010000176 DAPHCV010000000
VERSION     DAPHCV010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.178"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(114..2147)
                     /gene="betT"
                     /locus_tag="RH033_004346"
     CDS             complement(114..2147)
                     /gene="betT"
                     /locus_tag="RH033_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HDV2993435.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKNLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2276..2863
                     /gene="betI"
                     /locus_tag="RH033_004347"
     CDS             2276..2863
                     /gene="betI"
                     /locus_tag="RH033_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HDV2993436.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAELRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKALDKPLAHSLTRHFITQHLPTD"
     gene            2877..4349
                     /gene="betB"
                     /locus_tag="RH033_004348"
     CDS             2877..4349
                     /gene="betB"
                     /locus_tag="RH033_004348"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HDV2993437.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPSLEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFIPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4363..6051
                     /gene="betA"
                     /locus_tag="RH033_004349"
     CDS             4363..6051
                     /gene="betA"
                     /locus_tag="RH033_004349"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HDV2993438.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTTVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAKEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRAAANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHDLPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GAECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMAVVDAEGRVHRLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKMADMIRGQEALPRSTARYFVANGMPVRAKKMSRDVN"
BASE COUNT         1592 a         1795 c         1793 g         1346 t
ORIGIN      
        1 aggaatttca ggatatgaca tgacaatcat atataatata tgcataattt tttacagcga
       61 gtcaatgatt cccgcgccat atgcagacat ggcgcggttt tatgcaataa caatcacgcg
      121 tccgggaaca tcacgctatg gcccggcgct tcacgatgga gatggataaa gttaaggtgc
      181 cgctcgtact ggtcgagaat atcggtgatc acctgctctt tgctgtagtc catcaggtcg
      241 ttgccctggc tgccttctaa caggaaggtt tccagccggt agtaggtcga tttcccgcta
      301 cgtgcgcggt aggtaaagcc cggcaccgaa tattgctgcg gccaaatctg atagacaaag
      361 ttttgctctt cgcccatatg caccaacaaa tccagatgcc ccaactgttg tccctcttcc
      421 ggcggcaggt tttttagctc cacgtacgcg ccgcgcaacc gcagctcctg cgccacttct
      481 tccattgccg ggtaacagac cgtctccatc atctgtttag tgtaacgcgt gcccggataa
      541 ttcatcaggc gcgagagacg ttttttccag ctcaggcgat cctgaagccc cagcggtcgc
      601 ggcgcggtat cgcggttggc actttcacgg cggtaatctt ctaccttcag agatttatac
      661 aaccccgcca tcacgaagaa aatcacaaag ctgaacggca gccccatgat taccgtggtg
      721 ttttgcagcg cggatatccc gttggtcatc agcatgccga gcgtcagcag gccaatcgcc
      781 accgaccaga agacgcgcag ccagccgggg gcgtcgctgt tgatatcttt aagctgcgag
      841 gtgaaattcc ccagcaccag cgcaccggag tccgccgagg tcacataaaa cagcaggcca
      901 gtaatggtgg cgacggaggc gctaaaggta aacgccggat actgcgccag caggctgtag
      961 aagccgcgct ccggatggac catcgcttcc tcggcaaatg ccgcgccgcc gtggatgatt
     1021 tcatacagcg cgctattgcc gaacaccgag agccataaca gcgtgaaggt aaacggaata
     1081 atcaacgtgc ccagcacgaa ctggcgaatg gtacgcccgc gcgagatacg cgccagaaac
     1141 aggccgacaa acggcgacca tgccacccac catgcccaga agaagagcgt ccagttattc
     1201 atccactcaa ccggacggtc gaaggcaaaa ctgttgagcg tcatgcccat aaagcgattc
     1261 acatagtcgc caacgttcag caccagcgca ttaagcagga acgaggtgtc gcccataaac
     1321 agcacgaaaa ggatcaatcc cagcgccagc gcaacgttaa gttccgataa cacacgaatg
     1381 cccttatcga caccggaggt gacagagatc gtggcgatga tcaccgacaa ggcgatcagt
     1441 gccgctttcg ccgccattga atcgggaata tcaaacagta cgctcaagcc atagttaagc
     1501 tgcaccacac cgataccgag cgtagtggca ataccgaaga tggtgccgat taccgctgca
     1561 atatccactg aatgaccaat cggcccgtta atccgtttac cgaagatcgg gtacagcgcc
     1621 gagcggatgg tgagcggcaa attataacga tagctaaagt atccgagcgc catgcccatc
     1681 agcgcataca tcgaccagcc ggttaagccg tagtgaaaca gcgtccagac catcgcctga
     1741 cgcgcggcct caatcgtctg acccgcgcct tccggcggct gcatatactg cgttaccggt
     1801 tcggctacgg agaagaacat caggtcgata ccgatcccgg cagcaaacag catcgccgcc
     1861 cagctcagca ggctgaattc cggtttggat tgttctggcc cgagcttcac cgaaccaaaa
     1921 cgcgaacaag caatacagac cacaaagaca atatagagcg ttgccgccag cagatagtac
     1981 caaccgaagg ttttagaaac ccagtccagc gtgcggccaa tccacagggc cgagaagtcg
     2041 cgaaacagga tcgttgtcag ggaaaacaac aaaatcagtc cggcggaagt gtaaaacacc
     2101 accggattga ttttgtcctt ttccctgctg tgtgaaaggt ctgtcatcca gtatccccac
     2161 tgttattgtt accatttaaa atcaaattcg taacaattaa gacacatttt atattgaacg
     2221 tccaatcaat aaccgcttta atagataaac aacgctgatg aatggagtgg cgaaaatgcc
     2281 caaattgggg atgcagtcga tccggcgcag acaactgatc gacgccacgc tggaagcaat
     2341 aaatgaagtg ggcatgcacg atgcaacgat cgcgcagatc gcccgccgtg caggcgtttc
     2401 tacggggatc atcagccact atttcaggga caaaaatggt ctgctggaag caaccatgcg
     2461 cgatatcacc agtcagctac gtgacgcggt tttgaatcga ttacatgcac ttccgcaggg
     2521 cagtgcagag ctgcgattac aggcgattgt tggtggaaac ttcgatgaaa cccaggtgag
     2581 cagtgcggcg atgaaagcct ggctggcctt ctgggccagc agtatgcatc agccgatgct
     2641 ctatcgcttg cagcaggtca gtagccgccg cttattgtcg aatctggtga gcgaatttcg
     2701 ccgcgagtta ccgcgccaac aggcacagga agcgggctac ggccttgccg cgctgattga
     2761 cggattatgg ctgcgcgcgg cactgagcgg caaagcgctg gataaacctc tcgcacactc
     2821 gctgacccgt cactttatta ctcagcattt acccaccgat taaccgagga aacgtgatgt
     2881 cccgaatggc agaacagcag ctttatatac atggtggtta tacctccgcc accagcggtc
     2941 gcaccttcga gaccattaac ccggccaacg gtaacgtgct ggcgaccgtg caggccgccg
     3001 ggcgtgagga tgtcgatcgc gccgtgaaga gtgcccaaca agggcaaaaa atctgggcgg
     3061 cgatgaccgc tatggagcgc tcgcgtattc tgcgtcgggc cgttgatatt ctgcgtgaac
     3121 gcaatgacga actcgcaaaa ctggaaaccc tcgacaccgg aaaagcgtat tcggaaacct
     3181 caaccgtcga tatcgttacc ggtgcggacg tgctggagta ctacgccggg ctgatcccgt
     3241 cgctggaagg cagccagatc ccattgcgtg agacgtcatt tgtttatacc cgccgcgaac
     3301 cgctgggcgt agtggcaggg attggcgcat ggaactaccc gatccagatt gccttgtgga
     3361 aatccgcccc ggcgctggcg gcaggcaacg cgatgatttt caaaccgagc gaagtcaccc
     3421 cgcttaccgc gttaaagctg gccgaaattt acagcgaagc gggcctgccg gacggcgtat
     3481 ttaacgtgct gccgggcgta ggtgcggaaa ccgggcagta tctgaccgag catccgggca
     3541 ttgccaaagt gtcatttacc ggcggtgtcg ccagcggcaa aaaagtgatg gctaactcgg
     3601 cggcttcctc cctgaaagaa gtgaccatgg agctgggcgg taaatcaccg ctgatcgttt
     3661 tcgacgatgc ggatctcgat ctcgccgctg atatcgccat gatggcgaac ttcttcagct
     3721 ccggtcaggt gtgtaccaac ggcacccgcg tgtttattcc ggcgaaatgc aaagccgcat
     3781 ttgaacaaaa gattctggct cgcgttgagc gcattcgcgc gggcgacgtt ttcgatccgc
     3841 aaactaactt tggcccgctg gtcagcttcc cgcatcgcga taacgtgctg cgttacatcg
     3901 ccaaaggcaa agaggaaggc gcgcgcgtac tgtgcggcgg cgatgtactg aaaggcgatg
     3961 gcttcgataa cggcgcatgg gttgcaccga ccgtgttcac cgattgccgc gacgaaatga
     4021 ccatcgtgcg cgaagagatc ttcggtccgg tgatgtcgct tctcacttat gaatctgagg
     4081 acgaagtgat tcgccgcgcc aatgataccg actacggcct ggcggcgggc atcgtgacgg
     4141 cggacctgaa ccgcgcgcat cgcgtcattc atcagctgga agcgggtatt tgctggatca
     4201 acacctgggg cgaatcccct gcagagatgc ccgttggcgg ctacaaacac tccggcattg
     4261 gtcgcgagaa cggcgtgatg acgctccaga gttacaccca ggtgaagtcc atccaggttg
     4321 agatggctaa attccagtcc atattctaac caggaggttt atttgcaatt tgactacatc
     4381 attattggtg ccggctcagc tggcaacgtt ctcgctaccc gtctgactga agatccgaac
     4441 acaaccgtgc tgctgcttga agcaggcggc ccggactatc gctttgactt ccgcacccag
     4501 atgcccgccg cgctggcgtt cccgttacag ggtaaacgct ataactgggc ctatgagacg
     4561 gaaccggaac cgtttatgaa caaccgtcgc atggagtgcg gacgcggtaa ggggcttggc
     4621 gggtcatcac tgatcaacgg catgtgttac atccgtggta acgcgctgga tctcgataac
     4681 tgggcgaaag aacccggtct ggagaactgg agctacctcg actgtctgcc ctactatcgc
     4741 aaggccgaga ctcgcgatgt gggcgagaac gactaccacg gcggtgatgg cccggtgagc
     4801 gtcaccacct ccaaacccgg cgtcaatccg ctgtttgaag cgatgattga agcgggcgtg
     4861 caggcgggct acccgcgcac ggacgatctc aacggctatc agcaggaagg ttttggcccg
     4921 atggatcgca ccgtcacgcc gcagggccgt cgcgccagca ccgcgcgtgg ctatctcgat
     4981 caggccaaat cgcgccctaa cctgaccatt cgtactcacg ccatgaccga tcacatcatt
     5041 tttgacggca aacgcgcggt gggcgtcgag tggctggaag gcgacagcac cattccgacc
     5101 cgcgcggcgg cgaataaaga agtgctgtta tgtgcaggcg cgattgcttc accgcagatc
     5161 ctgcaacgct ccggcgtcgg caacgctgaa ctgctggcgg agtttgatat tccgctggtg
     5221 catgatttac ccggcgtcgg cgaaaatctt caggatcacc tggagatgta tctgcaatat
     5281 gagtgcaaag aaccggtttc cctctaccct gccctgcagt ggtggaatca gccgaaaatc
     5341 ggtgcggagt ggctgtttgg cggcaccggc gttggtgcca gcaaccactt tgaagcaggc
     5401 ggatttattc gcagccgtga ggaatttgcg tggccgaata ttcagtacca tttcctgcca
     5461 gtggcgatta actataacgg ctcgaacgca gtgaaagagc acggcttcca gtgccacgtc
     5521 ggatcgatgc gctcgccaag ccgtgggcat gtgcggatta aatcccgcga cccgcaccag
     5581 catccggcga ttctgtttaa ctacatgtcg cacgagcagg actggcagga gttccgcgac
     5641 gcaattcgca tcacccggga gatcatgcat caaccggcgc tggatcagta tcgtggccgc
     5701 gaaatcagcc ccggcgcgga atgtcagacg gatgagcagc tcgatgagtt cgtgcgtaac
     5761 cacgccgaaa ccgcgtttca tccgtgcggt acctgcaaaa tgggttatga cgagatggca
     5821 gtggttgacg ccgaaggccg cgtacacagg ctggaaggcc tgcgcgtggt ggatgcgtca
     5881 attatgccgc aaattatcac cggcaatttg aacgctacga cgattatgat tggcgagaaa
     5941 atggcggata tgattcgcgg gcaagaagcg ttgccgagga gcacggcaag atattttgtg
     6001 gcaaatggga tgccggtgag agcgaaaaaa atgagtcgtg atgtgaacta actcaggaac
     6061 ctatcagatt gagagttacc gttctactat gaggaccgaa tccttatata aaacactgag
     6121 gtaactctca tgcttcatat ccgcagtccc gccatcaaac tttaagccga ttcgctcaat
     6181 ctggcggatg atggtgctat ggatacgctg ataaaccaca agtgtaatgc cactcagctt
     6241 aagaatcacg caaccagtgc tcctggctag ggttacttgt tctttcccac tcccggttag
     6301 cagtggtcag cgatcaactg cgtaagccgg gcgtatttat cccaaagcgt ggagtaatat
     6361 caggtctacg ctaaaaaagt caatttattc ggttatcatg cacgaaaagg ggacttgtgg
     6421 cgagtataaa aataagtcag acccaaaaac tacgtggagc tttaaacacg gttcgtataa
     6481 tttattagca gcgatttatt tttacccttc tttaatcagt catcat
//
LOCUS       DAPHCV010000177         6377 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.179, whole genome shotgun sequence.
ACCESSION   DAPHCV010000177 DAPHCV010000000
VERSION     DAPHCV010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.179"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(115..1134)
                     /locus_tag="RH033_004350"
     CDS             complement(115..1134)
                     /locus_tag="RH033_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV2993439.1"
                     /translation="MAITRKRRSTGKVTIADVAQLAGVGTMTVSRALRTPEQVSDKLR
                     EKIEAAVNELGYMPNLTASALASASSHTIAMVVPNLAEAGCTEMFAGLQHVLQPAGYQ
                     IILAESRHRLEQEEKLLETLLASNIAAAILLSVEHNSTVRHLLKNASIPVIEIGAIRT
                     DPIDINIGIDNVAAMFELTDMLVQRGYQNIGLLCANQEQWIFQQHLQGWYKAMLRHHM
                     SPNRVINAALPPVFSTGASQLPEFLLAWPELDALVCVSDELACGVLYECQRRRIKVPD
                     ELAVVGFGDSDVSKVCQPSLTTMAVPHRKIGLEAGNVLLERIKEGNWSNQIFIEPCLC
                     LRESC"
     gene            1392..1835
                     /locus_tag="RH033_004351"
     CDS             1392..1835
                     /locus_tag="RH033_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDV2993440.1"
                     /translation="MLNIWLSDSTIALRKNAATWQQALDICARPLLDAEIITTDYLSA
                     IKTQHEKLGPYYVLAPGLAMPHARPEEGAKGLGLSLLKLEEGVSFHTEEFDPVDVIIL
                     LAAPDKHSHIEMISALAELFSSEKDLHELHQARNVEEIKKIISRF"
     gene            1914..2186
                     /locus_tag="RH033_004352"
     CDS             1914..2186
                     /locus_tag="RH033_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDV2993441.1"
                     /translation="MKIMAICGSGLGSSFMVEMNIKKVLKKMDIEAEVEHSDLSSATP
                     GAADLFVMAKDIAASASVPESQLVVINNIIDINELETQLRNWLAKQ"
     gene            2209..3597
                     /locus_tag="RH033_004353"
     CDS             2209..3597
                     /locus_tag="RH033_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDV2993442.1"
                     /translation="MFILETLNFVVDILKVPAILVGLIALIGLVAQKKSFSDVVKGTI
                     KTILGFIVLGGGATVLVGSLNPLGSMFEHAFNIQGIIPNNEAIVSIALEKYGASTALI
                     MAFGMVANIIVARFTRLKYIFLTGHHTFYMACMIGVILTVAGFEGISLVFTGSLILGL
                     VMAFFPALAQRYMKRITGNDDIAFGHFGTLGYVLSGWIGSVCGKGSRSTEEMNLPKNL
                     SFLRDSSISISLTMMVIYLIMAISAGREFVESQFSGGQNYLVYAIIMAITFAAGVFII
                     LQGVRLILAEIVPAFTGFSEKLVPNARPALDCPVVYPYAPNAVLVGFLFSFFGGLVGL
                     FLLGQMKLVLILPGVVPHFFTGAAAGVFGNATGGRRGAMIGAFANGLLITFLPVLLLP
                     VLGAIGFANTTFSDADFGAVGIVLGNLARYLSPFAITGLVVALFVLLVAWNIFASNKI
                     AAKAQKKSGAKS"
     gene            3594..4424
                     /locus_tag="RH033_004354"
     CDS             3594..4424
                     /locus_tag="RH033_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDV2993443.1"
                     /translation="MNVKELTQFARDIRIQTLKSLTHLGFGHYGGSMSVVETLAVLYG
                     AVMKIDPAAPDWPERDYFILSKGHAGPALYSTLALKGYFPLEELSTLNQNGTRLPSHP
                     DRLKTCGVDSTTGSLGQGISIAGGMALSHKLAGRKNRVFCIVGDGELNEGQCWEAFQF
                     IAHHRLNNLTVFVDWNKQQLDGQLDEIINPFDLEGKFRAFGFDVVTVKGDDIEGLLNV
                     VKPVLPAEARPRVVILDSIKGQGVPCLEQLSNSHHLRLTEEMKGTLNETIRQLEAMHD
                     "
     gene            4417..5370
                     /locus_tag="RH033_004355"
     CDS             4417..5370
                     /locus_tag="RH033_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HDV2993444.1"
                     /translation="MINLAPAGQKDSIEMRKVYADFIAQQSKNVSSVIALEADLMSSM
                     AMDSVAQDYPDHVINCGIMEANVIGTAAGLALTGRKPFVHTFTAFASRRCFDQLFMSL
                     DYQGANVKVIASDAGVAACHNGGTHMSFEDMGIVRGLAHSVVMEMTDAVMFSDILRQL
                     VALEGFYWIRTIRKQAASIYAPGTTFTIGKGQVLREGTDVTLIANGIMVVEALEAARQ
                     LELAGVSVAVIDMFTLKPVDRMLIKNYAEKTGRIVTCENHSIHNGLGSAVAEVLVETC
                     PVPMRRVGVKERYGQVGTQDFLQREYGLTAQDIVAAARELL"
     gene            5633..5746
                     /locus_tag="RH033_004356"
     CDS             5633..5746
                     /locus_tag="RH033_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV2993445.1"
                     /translation="MVKLVLQPGASVARIAREHDINDNLLFKWLRLWQNVR"
BASE COUNT         1669 a         1327 c         1553 g         1828 t
ORIGIN      
        1 ttcagggtgt catcacgcct tatgatgaca cccttcccct ggttgtggaa aaccgaagga
       61 tatatcaggt tcagcaacaa aatcagtgag ctgaaagata ccagtccccc agcttcagca
      121 gctttcacgt aaacataaac agggctcaat gaatatctga ttgctccagt taccttcttt
      181 aattcgttcc agcaaaacat ttcctgcttc cagaccaatt ttcctgtgag gaacggccat
      241 tgttgttaat gatggttggc aaactttgct gacgtcactg tcaccaaatc ctactacggc
      301 gagctcgtct ggaactttga tccgtcttcg ttgacattca tagagaacac cgcaggctaa
      361 ctcatctgac acacatacca gggcatccag ttccggccat gcgagcaaaa attccggtag
      421 ttgtgatgct ccggtggaaa aaacaggtgg cagagcagcg ttaataacac ggttgggaga
      481 catatgatgg cgtaacatag ctttatacca tccctgtaaa tgctgctgaa agatccactg
      541 ttcctggtta gcgcatagca aaccgatatt ctgataacca cgttgtacca gcatatccgt
      601 cagctcgaac attgctgcaa cattatcaat accaatgttg atatctatcg gatcagtacg
      661 aatggcaccg atttctataa caggaatcga ggcatttttg agcaagtgac gcacggtact
      721 attatgctca acactaagca aaattgctgc agcgatatta gaagccagca gggtttccag
      781 cagtttttct tcttgttcca gacgatggcg tgactcagcc aggattattt gataccccgc
      841 tggttgtagc acatgttgca gcccggcaaa catttccgtg catcccgctt cagccaggtt
      901 tggcacgacc attgcgatcg tgtgtgaaga tgccgatgcc agtgcgcttg cagtgaggtt
      961 gggcatatac cctaattcat taactgcggc ttcaatcttc tccctcaatt tatctgaaac
     1021 ttgttcggga gtgcgtaacg ctctggatac agtcattgtg ccaactcccg cgagttgcgc
     1081 gacatcagca atagtgactt tacctgtact acgacgtttt cgggttattg ccatacaaaa
     1141 tcctgctcag cgactacata tggagatctt ttattactta tcatcaacgg cttcctgcac
     1201 tgccagtagc agagtatacc tcttaatatc tgccataagt ctggatttaa tcagtgaaat
     1261 ctattttgat agcgctatca caaattcgaa caatacctcc tgatagcgct atctttgtga
     1321 tattgatcgc agatataccg tcgttacttg aatacattct ggtcatataa ataagaaatt
     1381 agggggtggg agtgcttaat atctggcttt ctgattcaac aatcgcatta cgaaagaatg
     1441 ccgcaacctg gcagcaggca ttagatatct gtgccagacc gttactggat gcagagataa
     1501 ttacgacgga ttatctcagt gcgataaaga cacaacatga aaaactgggg ccttattatg
     1561 tcctggcacc cgggctggcc atgccgcatg cccgaccaga agaaggggca aaaggattag
     1621 gtttatcatt attaaaactt gaagaaggtg tcagttttca tacagaggaa tttgatcctg
     1681 tagatgttat cattttactt gccgcaccag acaaacacag tcatatcgaa atgatatctg
     1741 cactggctga gttattctcc agcgaaaaag atttacatga attgcatcag gctcgcaatg
     1801 tggaggaaat aaaaaagata atttcacggt tctgattaca ttataaattc agtcttttca
     1861 caacattgcg ccgttgcgtg atgggacttc ttcaacttaa aatggtgaat ataatgaaaa
     1921 ttatggctat ttgtggctca gggcttggta gtagttttat ggtcgaaatg aatattaaaa
     1981 aagttctgaa aaaaatggat atagaggcgg aagtggaaca ttccgatctc tcatcagcaa
     2041 cgccaggggc tgccgattta tttgtgatgg ccaaagatat tgctgccagt gccagcgtac
     2101 cggaaagtca gttggtggtt atcaacaaca tcattgatat taatgaactc gaaacgcaac
     2161 tgcgtaactg gttagcaaaa caataacctt aattgaagcg aggtggatat gtttatcctt
     2221 gaaacgctga attttgttgt tgatatactt aaagtgcccg cgatactggt cggcttgatt
     2281 gccttaattg gtctggttgc ccagaaaaaa tctttttcag atgttgttaa aggtacaatt
     2341 aaaactattc tcggctttat tgtactgggc ggtggtgcca ctgttttggt tggttcgtta
     2401 aatcctcttg ggagtatgtt tgaacatgcc tttaatattc agggcatcat tccgaataat
     2461 gaggctattg tttctattgc gctggaaaaa tatggcgcat cgacggccct tattatggca
     2521 tttggtatgg tggcgaatat tattgttgcc cgctttaccc gccttaaata tatcttcctg
     2581 accggacacc acacatttta catggcctgc atgattggtg tgattctgac agtggctggt
     2641 tttgaaggta tatcactggt ctttaccggt tctcttattc tgggacttgt gatggcattt
     2701 ttcccggcat tagcacaacg ctatatgaag cgtattacgg ggaatgatga cattgctttt
     2761 ggtcactttg gcactctggg atatgtactt tctggctgga ttggcagcgt gtgcggtaaa
     2821 ggttcccgct ccaccgaaga gatgaatttg cccaaaaatc tgagcttcct gcgcgacagt
     2881 tccatctcta tttctcttac gatgatggtg atttatctga tcatggcgat cagtgctggt
     2941 cgtgagttcg ttgaaagcca gttcagtggt ggtcaaaact accttgtcta cgccatcata
     3001 atggccatca ccttcgctgc tggtgtattt attattctgc aaggcgtgcg tttaatcctt
     3061 gctgaaatag ttccggcatt taccgggttt tcagaaaagc tggtgccgaa tgcccgtccg
     3121 gccctcgatt gcccggttgt gtatccgtat gcgccaaacg cagtgttagt tggtttcttg
     3181 tttagctttt ttggcggtct ggtagggcta tttttgttgg gacagatgaa actggtgttg
     3241 attcttccgg gtgtcgttcc tcactttttt accggggcgg ctgctggcgt atttggtaac
     3301 gctacggggg gacgtcgtgg cgcaatgatt ggtgcttttg cgaatggctt attgattacc
     3361 ttcctgccag tactgttatt gccggttctt ggggcgatag gctttgctaa tacgaccttt
     3421 tccgatgcag attttggtgc tgtcggcatc gtcctcggaa acctggcacg ttatctctca
     3481 ccttttgcca tcaccggact tgtagtagcg ctatttgtgc tgctggttgc ctggaacatt
     3541 ttcgccagta acaaaatcgc tgccaaagcg cagaagaaga gtggagccaa atcatgaatg
     3601 ttaaagaatt gactcaattt gcacgggata tacgtatcca gacgcttaaa tcattaactc
     3661 atctcggctt cggtcattat ggtggcagca tgtcggttgt ggaaacgctg gcagtgcttt
     3721 atggcgctgt aatgaaaatt gatcctgctg ctcccgactg gcctgagcgt gactatttta
     3781 tcctgtccaa aggacatgcc ggacctgctc tctacagtac tctggcgctg aaaggctact
     3841 ttccactgga agaattaagc accttaaatc agaacggaac ccgcttacca agtcatccgg
     3901 atcggctaaa aacgtgtggt gttgatagca ccacaggatc gctggggcag gggatatcca
     3961 ttgccggagg gatggcgctg tcgcataaac tggcggggcg caagaatcgg gttttttgta
     4021 ttgtcggcga tggcgaactt aatgaaggcc aatgttggga agctttccag tttatcgcgc
     4081 atcatcgttt gaataacctc actgtgtttg tggactggaa caaacagcag cttgatggtc
     4141 aactggatga aattattaat ccatttgatt tggaaggaaa attccgggct ttcggttttg
     4201 atgtcgtgac agtgaagggc gatgatattg agggcttgtt gaatgtagtt aaacctgtcc
     4261 tgccagctga ggccagaccg agagtggtca ttctcgatag cattaaaggg cagggggtgc
     4321 cgtgtctcga gcaactcagt aattctcatc acttacgcct cacagaagag atgaagggga
     4381 cgctaaacga aacaattcgc caactggagg ctatgcatga ttaatctcgc accggcagga
     4441 caaaaagaca gcattgaaat gcgcaaagtt tatgcagatt ttatcgcgca acagagtaaa
     4501 aacgtcagtt ctgtcattgc tcttgaggct gatttaatga gttccatggc aatggacagc
     4561 gtggcgcagg attatccaga tcacgtcatt aattgcggca ttatggaagc caatgttatc
     4621 ggcacagctg cagggctggc cttaaccggg cgcaagccat tcgtgcatac ctttaccgca
     4681 tttgccagtc gtcgctgttt tgatcaactc tttatgtcgc ttgattacca gggcgctaac
     4741 gtcaaagtta tagcttctga tgcgggtgtt gctgcttgcc acaatggcgg aacacatatg
     4801 tcatttgaag atatgggaat tgtgcgcggg ctggcgcatt cggtggtgat ggagatgaca
     4861 gatgccgtaa tgttcagcga tatcttgcgt caactcgttg cactggaagg attttattgg
     4921 attcgtacca ttcggaagca agcagcgagt atttacgcac caggtacaac ctttacaatt
     4981 ggcaaagggc aggtattacg tgaaggtacg gatgtcacct taattgctaa tggcattatg
     5041 gtggtggaag ccctggaggc cgcacgtcaa cttgagctgg ctggggtaag tgtagcggtg
     5101 attgatatgt ttacgctcaa acccgttgat cggatgttaa ttaaaaatta tgccgaaaaa
     5161 acaggacgga tcgtaacatg tgaaaaccat agtattcata atggattagg ttcagcggtt
     5221 gctgaagtgc ttgtagaaac ttgtcctgtg cccatgcgtc gggtgggggt taaagagcgg
     5281 tatggccagg ttggaactca ggacttttta cagagagagt acgggttaac cgcgcaagat
     5341 attgtggcgg cagccagaga gctattgtaa ttcatggagt ttgtttacca ttctgttttt
     5401 cttaaaagtg agtgtctgtg cggtcactaa actcggtaat aaagcagttc attgcaggat
     5461 aagaagcgtt tggttgtgtc gttttaccgt aagaaccgac cgccgtatgt agccattaag
     5521 ccagtattgg taacgtaggt gcccaccttt tcaaactggt ggacgcctgt tatggagcaa
     5581 aaacattatc cgcagaaccc cgcagatcat tttcaaatga gttaaacttc aaatggttaa
     5641 actggttttg caaccaggag cctctgttgc ccgtattgcc cgggaacacg atatcaatga
     5701 taacctcctg ttcaaatggc tcagactctg gcagaacgta aggtaaaggg agatgaaaaa
     5761 ggcaatagca gaactgagtg acagtgaaac cattctggcg tacagggttg gatggtgaaa
     5821 ctgataaaaa accccctctg aaattctgtc agaggggcta gtcaataagg gaataatatt
     5881 caacatgaag atgtcttctg ggaatgttta tatagcaaat gctttcactc cggtaattaa
     5941 tttccatgat taactgcttt cgatcaataa aggaattgca aaagtgtgat cttgttcacg
     6001 acatcgtgga taataatatg cctgatgtct gaatccggaa acagtgctct ggcgggtttc
     6061 atgtgaagtt gacggataaa aaatccgcac gaccagacag acgtgcggtt tacgaaattc
     6121 gtaacaagag gcttttctac ggatcacgtg gtgcgtgcca cgtgaaattt ttgtagcgca
     6181 aaatgtgtga tgtgtaaata caaggaagga aaaaagttaa atcggtggaa gtccatacct
     6241 gccacgtgta cgtctgcatt cctcgttttg aacagaattc tcaccagaca tcgtgaattt
     6301 ctcattcaat catcagagat attaaccagg atttttattg ttttgtgata gcagtacttt
     6361 aaatgttcag atgctaa
//
LOCUS       DAPHCV010000178         6212 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.180, whole genome shotgun sequence.
ACCESSION   DAPHCV010000178 DAPHCV010000000
VERSION     DAPHCV010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.180"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1671
                     /locus_tag="RH033_004357"
     CDS             <1..1671
                     /locus_tag="RH033_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDV2993446.1"
                     /translation="FPLNRAGSVWRGQGNLMMHTEDGSAWEVALASSQQNKHTELTPG
                     APGLGKSVLINALSEIQIASAQKNLPFIAYIDKGFSAQGLVQLIRDSLPEQRKDEAVG
                     IILSNDPEHTRNLFDVMYGAKKPATPEKNFMVSVLCALCVDTGTGQPCNPGDTRQIIS
                     NLVDLAFREYGENNPRLYRAGTEPLVDLALEESGIAEQHDAGWWNAATWFEIRDMLHM
                     AGNIPAAQRAHYQAMPLLAEMSALLGQPSIRDVFGTVQRDNSAELLLDYIRRALDQGH
                     SDYPMMSGCTRFMLSPDTRVVAVDLNNVAGDKTPAGRLRTGIMYLLAGQIAGGDFVLP
                     QYQEEIRKNLDPRYHEVIFRRIEQLDQEVKTKVYDELHNAKGINFIWEALDTQELEQR
                     KFGIRTVLSTQLLQDYPPAVLKSANTLWLLRYRPDEIPFLRDNFGVPEVTLRRFLKMP
                     EGAAPDGSGVPVLAVFRVKNGTLARILKFTLGPLELWALNSSPKDSALRRALTQELGS
                     LRARQILAENFPRGSATSLIEHRARTHDSENVIHDLAAELIRKQGYHL"
     gene            1671..2291
                     /locus_tag="RH033_004358"
     CDS             1671..2291
                     /locus_tag="RH033_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraV"
                     /protein_id="HDV2993447.1"
                     /translation="MYSIHITPRCRRPARGQVFLKTLRVDGDSFSFTLPSACFTLRPS
                     PNPRVIFASDRRADGEMSGVRLALPGETGTLITTATWQVNVPERPPFDIQHVIHWLLP
                     SPLTALMSDNTWLWPNSPGTPALTAGDWPVMETDTPTLKRVKRRTFTLIDNRNRQGWI
                     TRRFQCFPLAPLPESHSHRVPDILYPLKTEKKECKDNQQPHCLHSW"
     gene            2249..3454
                     /gene="traW"
                     /locus_tag="RH033_004359"
     CDS             2249..3454
                     /gene="traW"
                     /locus_tag="RH033_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HDV2993448.1"
                     /translation="MQRQPATTLLALLVGCAVIPSTHAYSVTVVASQPVTTQIMPELA
                     AIQGVMAEMVRMQTATGTAINQSSEKLASVIAQDGQATRQQMIFSNETRRLEEARKSF
                     SVPDSICSESASGVAAESRRAAASAAARLSQGGGVSSKPIRERLSRAADSPTREAYDS
                     AGIHAGYCTEAEYARFGGTDVCPAVGELPGGDSQVRSLYQGAGTADTPAALTWDQKQI
                     DAATAYMKNTARPSAGRAPGKGEVGTQTGRTYVGLQNEYNGIIDAASHPQLSLIADST
                     PNEATRGALTEALQSPSAAAYFDRTASSEARTRGHMSQREFEAFEAGRRYANTDWQQD
                     LQGMEGDNLLRELLRTTALLNWQMNDLKEQIRQGNVIAGQQLALAARQYYGQRLGELS
                     QAMSQGSVR"
     gene            3451..4020
                     /gene="traX"
                     /locus_tag="RH033_004360"
     CDS             3451..4020
                     /gene="traX"
                     /locus_tag="RH033_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HDV2993449.1"
                     /translation="MKDDNSTDKKKPAPRLRRIGDIGINLFAPLAETRWMLRSCRHML
                     GQHKTRLKRLMPDRQPESPVCMTWEEAVRASGLTVNELDRRLRRRRMFWRTGFWLMMI
                     PVLLLCGMALAATALPAITLIRILACLLLMSAIAALCASKTLIVSYRLWQLNERRVSE
                     DEQGTFPDYLQARHGWKQATWLAITHRQY"
     gene            4093..>6212
                     /locus_tag="RH033_004361"
     CDS             4093..>6212
                     /locus_tag="RH033_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HDV2993450.1"
                     /translation="MKIIPRALSAGLALAAVPAQAFVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLTASSNTIIGNLFMVFNAIIAIMGIVWFLFIGIRHVVRTGHQGNVFNN
                     GRDVVGMLSIVSGFMMIVPTASGWSLAQLVMLWGASIMGIGSANIMVQTAAENIAEGY
                     SMTVQPVHVSTRTAARGILEMALCKHAMNAGLDDFNRTARSSTPPMSESSRTENGRYT
                     VTVSNGSATCGSVSLTVKGNGSNKQSALDYFFNPFIETEYQGVLTAQRSAMDTMLRDL
                     DSAAQEFVSAYRTRRDTGNGNLPDIESRIQKAANRYEETVQKALPTDNAETERREQLK
                     SYLTTYGWVALGAWYQTFATANQRLAELADRAPTVSGVSSQGEAGDTEFLKEVKGAYQ
                     AQLQNSPYTPALGNLTSSDEIYAANTNDPQSAIIKLMSPDFTKLTYKIATVYSSTGTT
                     SYQANPLLKMKDIGDYTMVTTETIWAGYTTVRVLSTAGSNSVFGRLVNAVSGLSDAAK
                     AFLEASAPPLYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCAAGPLWA
                     ATHLGTSQDRGSRAAYGYIYLIDSMIRPLLMVFGFFFASVAIVAGGTILNALFAAALM
                     NVQINSVTGVFSMAGFLMIYARACTTLVAAIFALQAYLPDHVINFLGGRDGVNMLGNM
                     VSSVKDIIAGGNTNIRRTPGVREE"
BASE COUNT         1432 a         1813 c         1704 g         1263 t
ORIGIN      
        1 tttcccctga acagagccgg ctctgtctgg cggggacagg gaaatctgat gatgcacacc
       61 gaggacgggt ctgcatggga ggtcgcgctg gcatcgtcac aacagaacaa gcacacggaa
      121 ctgacgcccg gcgcacccgg tctgggcaaa tcggtactca tcaacgcact gtcggagatc
      181 cagatagcct cagcccagaa gaacctgcct ttcattgcct atatcgacaa gggattcagt
      241 gcacagggac tggtacagct tatccgtgac agcctgccgg agcagagaaa ggacgaagcc
      301 gtggggatca ttctgagcaa tgatccggaa cacacccgta acctgtttga tgtgatgtac
      361 ggtgctaaaa aacccgccac cccggaaaag aacttcatgg tatctgtact ctgcgcactg
      421 tgcgtggata ccggcaccgg tcagccctgt aatcccggtg acacccggca aatcataagc
      481 aacctggtgg atctggcctt cagggagtat ggcgaaaaca accccagact gtaccgtgca
      541 ggaacagagc cccttgtgga tctcgccctg gaagagagtg gcatcgctga acaacatgac
      601 gcaggatggt ggaatgctgc cacctggttt gaaatcaggg acatgctgca tatggctggc
      661 aatatcccag ccgcccagcg ggcacactac caggccatgc ccctgctggc tgaaatgtcc
      721 gccctgctgg gacagccatc catccgggat gtgttcggca cagttcagcg cgataactca
      781 gcagaattgt tactggacta catccgccgt gccctcgatc agggacacag tgactacccg
      841 atgatgtccg gctgtacccg gtttatgctc agccccgaca cccgcgttgt ggccgttgat
      901 ctgaacaatg tggccggtga caagacacct gcagggcgtc tgcgcaccgg catcatgtac
      961 ctgctggcag gacaaatcgc cggcggggat tttgtcctgc cgcagtatca ggaggaaata
     1021 cggaaaaatc tcgacccacg ctaccatgag gtgattttcc gccgcattga acagcttgat
     1081 caggaggtca agaccaaagt ctatgacgaa ctgcacaatg caaaagggat caatttcatc
     1141 tgggaagccc tggacaccca ggagctggag cagcgtaagt tcgggatacg cacagtgctg
     1201 agcacccagc tactgcagga ctatccaccg gcagttctca aatccgccaa caccctctgg
     1261 ttgctgcgtt accgccctga cgaaattccg tttctgcggg ataattttgg tgtccctgaa
     1321 gtcaccctgc ggcgcttcct gaaaatgccg gaaggggctg caccggatgg cagcggtgtc
     1381 ccggtactgg ctgtgttccg tgtaaaaaac ggtacgctgg cccggatcct gaaattcact
     1441 ctcggtccac tggagctgtg ggcactgaat tcctcaccca aagacagcgc cctgagacgt
     1501 gcgctgacgc aggagctcgg gtcactgcgg gcacgccaga tcctggcaga aaactttccc
     1561 cgtggttcag ccacctcact gatagaacac cgcgcccgta cacatgacag tgaaaacgtc
     1621 atccatgatc tggctgcaga actcatccgt aagcagggat atcacctctg atgtacagca
     1681 tacacatcac tccccgctgc cggcgtcctg cacggggaca ggtcttcctg aaaacgctgc
     1741 gcgtggacgg tgacagtttc agcttcaccc tgccgtccgc ctgcttcacg ctgcgtccct
     1801 cccccaatcc ccgggtcatt ttcgccagtg acagacgggc tgacggggaa atgagtggcg
     1861 ttcgcctggc tctgcccgga gagacaggca cactgataac aacggccacc tggcaggtca
     1921 acgtgccgga gcggcctcct tttgatatac agcatgtcat tcactggctc ctgccctcgc
     1981 ctctcacggc gctgatgtcc gacaacacct ggctgtggcc gaatagcccc ggcacaccgg
     2041 cactgacggc gggagactgg ccggtaatgg aaaccgatac cccgacgctg aaacgcgtga
     2101 agcgtcgtac cttcacgctc atcgataacc gcaaccgtca gggatggatc acccgtcgct
     2161 tccagtgctt cccgctggct ccgctgccgg aatcacacag tcaccgcgta ccagacatcc
     2221 tgtatcccct gaaaacggag aaaaaagaat gcaaagacaa ccagcaacca cactgcttgc
     2281 actcctggta ggctgtgcgg ttatcccgtc cacccacgcc tattctgtga ccgtagtggc
     2341 cagccagcct gtcaccacac aaatcatgcc ggagctggcc gccattcagg gcgtgatggc
     2401 agagatggtc cgtatgcaga cagccaccgg cacagccatc aaccagagtt cagagaaact
     2461 ggcctctgtc attgcgcagg atgggcaggc cacccgtcag cagatgatat tcagtaatga
     2521 aacccgccgg cttgaagaag cccggaagag cttcagcgtg ccggactcca tctgcagtga
     2581 atccgcctcc ggagttgcag ctgaaagtcg ccgtgcagca gcgtctgcgg ctgcacgtct
     2641 gagtcaggga ggaggcgtca gcagtaaacc catccgtgaa cgtctctccc gggcagcaga
     2701 ctcaccaacc cgcgaggcat atgacagtgc aggcattcat gccggttact gcacggaagc
     2761 tgaatatgcc cgctttggcg gaaccgatgt ctgccctgcc gtgggcgagc tgccgggcgg
     2821 agacagccag gtgcgatcgc tctatcaggg tgcaggcaca gcagataccc ctgccgccct
     2881 cacctgggat cagaaacaga ttgatgctgc cacggcttac atgaaaaaca ccgccagacc
     2941 gtcagcgggt cgtgccccgg ggaaaggtga agtgggaaca cagaccgggc gcacatatgt
     3001 gggcctgcag aatgaataca acggcatcat cgatgccgct tctcatccgc aactgtcact
     3061 gatagctgac agtaccccta atgaggccac ccgtggagcg ctgactgaag ccctgcagtc
     3121 accatccgct gcggcatatt ttgaccggac cgcatcatca gaggcccgca cccgtggtca
     3181 catgagccag cgggagtttg aggcctttga agctggcaga cgctatgcaa acaccgactg
     3241 gcagcaggat ctgcagggca tggaaggcga taaccttctc cgtgaactgc tgcgcaccac
     3301 cgccctgctg aactggcaga tgaacgacct gaaagagcag atacgccagg gtaatgttat
     3361 cgccgggcag cagctggcac tggcggcacg acagtattac ggacagcgtc tcggtgaact
     3421 gtcacaggcc atgagccagg ggagcgtaag atgaaagacg acaacagcac cgacaaaaaa
     3481 aaaccggccc cccgtctccg ccgcatcggt gacatcggga taaacctgtt cgcaccactg
     3541 gcagaaaccc ggtggatgct gcgcagctgc cgccatatgc tgggccagca taaaacgcgc
     3601 ctgaagcgcc tcatgcccga ccggcaaccg gagagtccgg tctgtatgac ctgggaagaa
     3661 gccgtcaggg ccagcggcct tacggtcaac gaactggatc ggcgtctgcg acgtcgcagg
     3721 atgttctggc ggacaggatt ctggctgatg atgataccgg tacttctgct gtgcggcatg
     3781 gcccttgctg ccacagcgct tccggccatc accctgatac gtatactggc ctgcctgtta
     3841 ctgatgagtg ccattgcagc actgtgtgcc tcaaaaaccc tgattgtgtc ataccgcctc
     3901 tggcaactga atgaacggcg ggtcagcgag gacgaacagg gcaccttccc cgattacctg
     3961 caggcccggc acggctggaa acaggccaca tggctggcca ttacccaccg gcagtactga
     4021 ccgtccggtc tgcaattcct tttcctcatt ccggaggctg atgccctgca tcccttcggt
     4081 tccggagtta tcgtgaaaat catacccaga gcactgagtg cgggtctggc gctggctgcc
     4141 gtacctgcac aggcattcgt cacctatcag gacattgtga gcgccgccac caatccggat
     4201 gacctgtcgc gccaggcact ggtcaccatt tttggcgacg tggtcacaaa tcccctgaca
     4261 gccagcagca acaccattat cggcaacctg ttcatggtgt ttaacgcaat tatcgccatt
     4321 atggggatag tctggttcct gtttattgga atccgccatg ttgtccgcac cggccaccag
     4381 ggcaatgtct ttaacaacgg gcgggacgtc gtcggaatgc tcagtattgt gagcggtttt
     4441 atgatgattg tgcccaccgc ttccggctgg agtctggcac aactggtcat gctctggggc
     4501 gcatccatca tgggtattgg ttccgccaat atcatggttc agacggctgc agaaaacatc
     4561 gctgaaggct attcaatgac cgtacagcca gttcatgtct ccacccgcac ggcagcacgg
     4621 ggaatactgg agatggcgct gtgcaaacac gccatgaatg caggcctcga tgactttaac
     4681 cgtactgccc gttcatcaac gccccccatg agcgaatcct cacggacaga gaacggcagg
     4741 tataccgtca ccgtttcaaa tggcagtgcc acctgcggga gtgtgagtct caccgtgaaa
     4801 gggaatggca gcaacaagca gtccgcgctg gactattttt tcaatccctt cattgaaacg
     4861 gagtatcagg gagtcctgac ggcacaacgg tctgccatgg ataccatgct ccgtgacctg
     4921 gacagcgcag cacaggaatt tgtgtcagcg taccggaccc gacgcgacac gggtaacggc
     4981 aacctgccgg atattgaaag ccgtattcag aaggctgcca accggtatga agaaacggtg
     5041 cagaaggcgt tgccaacaga taacgcagaa actgagcgca gggagcagct gaaatcctac
     5101 ctgaccacct acggctgggt tgccctgggg gcctggtacc agacctttgc caccgccaac
     5161 cagcgactgg cagaactggc cgaccgtgcc cccactgtga gtggagtttc atcgcagggc
     5221 gaagccggag atactgagtt cctgaaggaa gtcaaaggag cttaccaggc acagttacag
     5281 aactcaccat atactcctgc gctgggaaat ctaacatctt ctgatgagat atatgctgcc
     5341 aatacaaatg atccacaaag tgcaattata aagttaatgt caccggactt tacgaaactg
     5401 acatataaaa tcgcaactgt atattctagt acaggaacta catcctatca ggcaaatccc
     5461 ctgctgaaga tgaaggatat cggtgattac accatggtca caacagaaac catctgggcg
     5521 ggttatacca ccgtcagggt gctttctact gccggcagca actccgtttt tggccgactg
     5581 gtcaatgccg tcagcggctt atctgatgca gcaaaggctt ttctggaagc ctccgcacca
     5641 ccactctact tcctgctgtt tttgctgttc tgtgccggtt tttcgttatc tatttacctg
     5701 ccgtttatcc cgtttatctt ctggatgacc ggcatcggca actggatagt cagcgtactg
     5761 attggatgtg ctgctggccc cctgtgggcc gccacccatc tgggaacaag tcaggaccgg
     5821 ggaagccggg ccgcttacgg ttacatctac ctgatagaca gcatgatcag accgctgctg
     5881 atggtgttcg gattcttttt tgcatcggtc gccatcgtgg ccggcggcac aatcctgaat
     5941 gcgctgtttg ctgctgcgct gatgaatgtc cagataaatt ctgtgacggg cgtgttcagc
     6001 atggcgggat ttttgatgat ttatgcccga gcctgtacca cgctggttgc ggccattttt
     6061 gcacttcagg cttatctgcc tgaccatgta attaattttc tgggcgggcg tgacggtgtt
     6121 aatatgctgg ggaatatggt ttcttccgtc aaagatatta ttgccggcgg aaacaccaat
     6181 atacgcagaa cacctggcgt tcgtgaagag aa
//
LOCUS       DAPHCV010000179         6119 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.181, whole genome shotgun sequence.
ACCESSION   DAPHCV010000179 DAPHCV010000000
VERSION     DAPHCV010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.181"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(23..802)
                     /locus_tag="RH033_004362"
     CDS             complement(23..802)
                     /locus_tag="RH033_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDV2993451.1"
                     /translation="MRQYRLLSPMIKVVIVLFSLLFSTAQAGLVIGGTRFAYPENQSS
                     ISVELKNTSDRDMLVKVAVSPDDARQLTGTVESLPSYSETVFIATPPLFVLKPDKNTK
                     IRINRVGGSLPTDRESLFILNVAALPTLENSHSVKTDNQLQIAVRNRMKIFYRPSNLS
                     EDPNMSYQKLRWARKNEIVTVYNPGPRYVTLYNLHVDGKVIDGGMVAPFSHRQQSWCK
                     SQGICEITWQTLDIYNNVLPALKVNMNPLQMDVSGSQFKTD"
     gene            complement(768..1340)
                     /locus_tag="RH033_004363"
     CDS             complement(768..1340)
                     /locus_tag="RH033_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV2993452.1"
                     /translation="MPGKQMLCCILISIISEGDMKIFISLFLFIISTNSFADDITHAG
                     VVRIEGLITEKTCIISDESKNFTVNMPDVPSSSVRSAGDVTEKVYFSITLTRCGSDVG
                     NAYIKFTGNTVSEDASLYKLEDGSVDGLALTIFDKNKGSISNDVKSIGFSLTSSVDNI
                     LHFFAAYKALKNNVQPGDANASVSFIVTYD"
     gene            complement(1291..1854)
                     /locus_tag="RH033_004364"
     CDS             complement(1291..1854)
                     /locus_tag="RH033_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV2993453.1"
                     /translation="MNLIFSKYSVFFILFAFFAFPLSGQANDSVVVTFTGILLAKSCD
                     ITTGSKDQSVKMGTYDANEFLNVGDVSPSQTFTINVQGCPTAKSTVYSSGVSANVRFT
                     GDADAINSALLRLSAGADSATGLGIEILDNNDVAIAINGESGFRDLVLNENGDANLTF
                     KLRYKSTQENVHAGQANALLYFDIDYQ"
     gene            complement(1854..4448)
                     /locus_tag="RH033_004365"
     CDS             complement(1854..4448)
                     /locus_tag="RH033_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HDV2993454.1"
                     /translation="MNLKLKRCEYWMAAQKQMKRVVPLLLVIMPACSIAGMRFNPAFL
                     SGDTEAVADLSRFEKGMTYLPGSYEVEVWVNDSPLLSRTVTFKADDENQLIPCLSLAD
                     LLSLGINKNALPEQALALSENSCLDLRIWFPDVHYMPELDAQRLKLTFPQAIIKRDAR
                     GYIPPEQWDNGITAFLLNYDFSGNNDRGDYSSNNYYLNLRAGINIGAWRFRDYSTWSR
                     GSNSAGKLEHISSTLQRVIIPFRSELTLGDTWSSSDVFDSVSIRGIKLESDENMLPDS
                     QSGFAPTVRGIAKSRAQVTIKQNGYVIYQTYMPPGPFEISDLNPTSSAGDLEVTIKES
                     DNSETVYTVPYAAVPILQREGHSKYSTTVGQYRSNSYNQKSPYVFQGELIWGLPWDIT
                     AYGGAQFSEDYRALALGLGLNLGVFGATSFDVTQANSSLVDGSKHQGQSYRFLYSKSL
                     VQTGTAFHIIGYRYSTQGFYTLSDTTYQQMSGTVVDPKTLDDKDYVYNWNDFYNLRYS
                     KRGKFQASVSQPFGNYGSMYLSASQQTYWNTDKKDSLYQVGYNTSIKGIYLNVAWNYS
                     KSPGTNADKIVSLNVSLPISNWLSSTNDGRSSSNAMTATYGYSQDNHGQVNQYTGVSG
                     SLLEQHNLSYNIQHGFANQDNSSSGSVGVNYRGAYGSLNSAYSYDNEGNQQINYGISG
                     ALVVHENGLTLSQPLGETNVLIKAPGANNVDVQRGTGISTDWRGYAVVPYATEYRRNN
                     ISLDPMSMNMHTELDITSTEVIPGKGALVRAEFAAHIGIRGLFTVRYRNKSVPFGATA
                     SAQIKNSSQITGIVGDNGQLYLSGLPLEGVINIQWGDGVQQKCQANYKLPETELNNPV
                     SYATLECR"
     gene            complement(4485..5237)
                     /locus_tag="RH033_004366"
     CDS             complement(4485..5237)
                     /locus_tag="RH033_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDV2993455.1"
                     /translation="MKCIIGNKKWVYGFRDELKISQNIIRSGILFLLSILFINNASAG
                     GFGLGVTRLIYPAESKQITLSVQNSGDNTSYLIQSWVDDSKTEKKSQDFVITPPLFML
                     PTRKEASLRIMFLGKTNLPTDRETLYWMNVKAIPPTDEKNTQKNTLQLALQNKIKLFY
                     RPENLPVQPGKARNMLRFKYEGKQLKVINPSPYYLTLTGIKVQGSKLPNTFVPPKSDI
                     TVSSAVTLAGEITYQTINDFGATTERQKGIMQ"
     gene            complement(5338..6042)
                     /locus_tag="RH033_004367"
     CDS             complement(5338..6042)
                     /locus_tag="RH033_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV2993456.1"
                     /translation="MKFNLSNLSAVLLASGMLMSTAVTAAPGDAAQFGGADTDWSTVD
                     YPRLTDMDDNVDSMGGKIRFTGRVVKATCKVATDSKQIEVVLPVVPSNLFTGIDIEAQ
                     GASNQTDFNINLTECSNTEDQKIEFRFTGTADSANKTLANEVEGSTDADNSGNAGATG
                     VGIRIYSKGTTNNGLINLNTTAAEGSASTAAYTIPGNATTHDFSAAFTAGYAQNGSTV
                     APGVVKSTASFVVLYE"
BASE COUNT         1807 a         1363 c         1125 g         1824 t
ORIGIN      
        1 gtaatcatac gatttccttt tactaatccg ttttaaattg tgagcctgac acatccattt
       61 gcaacggatt catatttact ttcaatgcag gcaatacatt attataaatg tccaatgtct
      121 gccatgttat ttcacaaatc ccttgcgatt tacaccatga ttgttggcga tggctaaatg
      181 gtgcgaccat accaccatct ataactttac catcaacatg taggttatag agtgtcacat
      241 atctggggcc tggattataa acggtaacaa tttcattttt tcgtgcccag cgtaattttt
      301 gatatgacat attcgggtct tcagacaaat ttgatggccg atagaatatt ttcatacgat
      361 tgcggactgc tatttgcaat tgattatcag ttttgaccga atgactattt tccaacgttg
      421 gtaatgctgc aacattcagt ataaataaac tttcccggtc ggtaggtaaa ctgccaccaa
      481 cacgattaat tcttattttt gtatttttgt caggcttcaa aacaaacagt ggcggagttg
      541 cgataaatac cgtttcagaa tatgagggca aagattcaac cgttcccgtc aactgacgag
      601 catcatcagg ggagactgca accttgacta acatatctct gtctgaagtg tttttgagtt
      661 caacagatat tgaggactga ttttccggat acgcaaaacg cgttccaccg atcactaagc
      721 ctgcttgtgc tgtagagaat aaaagggaaa ataatactat gactacctta atcataggtg
      781 acaataaacg atactgacgc attggcatcc cctggttgga cattattttt taatgctttg
      841 taagccgcaa aaaaatgcaa tatattatca actgatgatg taagtgaaaa accgatgctt
      901 ttaacatcat tactaatact gcctttgttc ttatcaaaaa tcgtgagtgc aagcccatct
      961 accgagccat cttccagctt atataaactg gcatcttcag aaactgtatt gccggtaaac
     1021 tttatatacg cgttgccaac atcactacca cagcgggtta acgttatgga aaaataaacc
     1081 ttttcagtaa catcccctgc actccttacc gaactactgg gtacgtctgg catattaact
     1141 gtaaaatttt ttgactcatc agaaataatg caggtttttt cggtaattag cccttcaata
     1201 cgaaccactc cggcatgagt gatatcatca gcaaaagaat ttgttgatat tataaacaaa
     1261 aataaactaa tgaatatctt catatctccc tcactgataa tcgatatcaa aatacaacag
     1321 agcatttgct tgcccggcat gtacattttc ctgagttgat ttatagcgaa gtttaaacgt
     1381 caaattagca tcaccgtttt catttaacac taaatcacga aaacctgatt caccattaat
     1441 agcaatcgcc acatcattgt tatccaatat ttctattccc agtccagtcg cactatctgc
     1501 accggcggag agacgcagta atgcgctatt aatggcatct gcatcaccag taaaccgcac
     1561 attggcgctc acaccagaac tataaacagt actttttgca gtcgggcatc cttgtacatt
     1621 aatcgtaaac gtttgactcg gagaaacatc acctacattt aaaaattcat tagcatcata
     1681 agtgcccatt tttaccgact gatctttact tccggtagta atgtcacagc tttttgccaa
     1741 caaaatccca gtaaatgtaa caacaactga atcattggcc tgccctgaga gcggaaaagc
     1801 aaaaaaagcg aaaagaataa aaaatacaga atatttagag aatattaaat tcattagcgg
     1861 cactccagag tagcatagct aacaggatta ttcagttctg tttcagggag cttgtaatta
     1921 gcctgacatt tttgctgaac accatctccc cactggatat taataacacc ttctaaaggc
     1981 aatcctgaga gataaagttg tccattatcg ccgacaatcc cggtaatttg actactattt
     2041 ttaatctgtg cgctggctgt agcaccgaat gggactgatt tattacgata acgaactgtg
     2101 aacaaaccac gaataccgat atgagcagca aactctgcac gaactaacgc accttttccc
     2161 ggaataactt cagtggaagt gatatccagt tcagtatgca tattcattga cataggatct
     2221 aatgaaatat tattacgtct atattctgtt gcataaggaa caactgcata tccacgccag
     2281 tcagtggata ttcctgtccc ccgctggaca tccacattat tcgctccagg cgcttttatc
     2341 aaaacattag tttcacctaa tggttgactc aacgtaagac cattttcatg tacaacaaga
     2401 gcaccactga tgccatagtt tatctgttga ttaccttcat tatcgtaact gtaggcggaa
     2461 ttcaaggaac catatgcccc acgataatta acaccaacag aaccactact gctattatcc
     2521 tgattagcaa aaccatgttg tatgttataa ctgagattat gctgctccaa cagagaacca
     2581 gatacccccg tatattggtt tacctgtccg tggttatcct gactataacc atacgttgca
     2641 gtcatggcat tcgatgatga gcgcccatca ttcgtggaag ataaccaatt acttataggt
     2701 aatgagacat ttagcgagac aattttatcc gcatttgtcc ctggtgattt actgtaattc
     2761 cacgcaacat ttagatagat acccttaata ctggtgttat aaccaacttg gtataaagaa
     2821 tcttttttat cagtattcca gtatgtttgc tgactagccg ataaatacat agacccgtag
     2881 ttaccgaaag gttgcgatac actagcctga aattttccac gtttgctata acgcaagtta
     2941 taaaaatcat tccagttata aacgtaatct ttatcatcta acgtttttgg atcaacaaca
     3001 gtccctgaca tttgttggta tgtcgtatca cttaaagtgt aaaagccctg ggttgaataa
     3061 cgatagccaa taatatggaa tgctgttcct gtctgaacta acgatttgga ataaagaaaa
     3121 cgataagatt gcccttgatg tttgctccca tccacaagcg aactgttagc ctgagtaaca
     3181 tcaaacgatg ttgcaccaaa tacacccaga ttcaggccaa ggccgagcgc caacgcccgg
     3241 taatcctcag agaattgtgc cccaccataa gccgtaatat cccagggtaa accccaaatt
     3301 aattccccct gaaatacata aggacttttc tggttatagc tattgcttcg atattggcca
     3361 accgtagtag aatattttga atgaccttct cgttgcagga tggggacagc ggcataaggt
     3421 acggtataga cagtttctga attatcagac tctttgatgg taacttccag atctcccgca
     3481 gatgatgtcg ggttaagatc gctaatctca aacggtcccg gcggcatata ggtttgataa
     3541 atgacataac cattctgttt gattgttacc tgagcgcgac ttttcgcaat tccgcgcacc
     3601 gtgggagcga aaccactttg actatcgggc aacatatttt cgtcagattc cagttttatg
     3661 ccacgaatac taacactgtc gaaaacatct gatgatgacc atgtatctcc tagcgttaat
     3721 tcacttctga aaggaataat aacgcgctgc aacgtactac tgatatgctc cagtttacct
     3781 gctgaattac tcccacgact ccaggttgaa taatcgcgaa aacgccatgc accaatattg
     3841 atcccagcgc gaagatttaa ataatagtta tttgaagagt aatcaccacg atcgttatta
     3901 ccagaaaagt cataattcag caaaaaagct gtaataccgt tatcccactg ttctggtgga
     3961 atatatccgc gggcgtcacg ttttattatc gcctgcggaa aggtcagttt aagtctctgt
     4021 gcatccagct ccggcatgta atgcacatca ggaaaccaga tacgcaaatc aaggcaacta
     4081 ttttcagata aagccaaagc ctgctctggc agcgcattct tgttaattcc aaggcttaat
     4141 aagtcagcaa gtgaaaggca gggaatcagt tgattctcat cgtctgcttt aaaagttaca
     4201 gtacgagaga gtaaagggga atcattgacc caaacttcga cttcatagct accaggaaga
     4261 taagtcatcc ctttctcgaa acgggataag tcagcaacag cttcagtatc acccgacaga
     4321 aaagcagggt taaagcgcat tcccgcgatt gaacatgcag gcataataac cagaagaagc
     4381 ggtacaaccc gtttcatctg cttttgtgcc gccatccaat attcgcatct tttgagcttt
     4441 aggttcatat ccctgacctg tgttgcatct gaactatccc gttgttactg cattattcct
     4501 ttctgccgct ccgtcgtagc gccaaagtca ttaattgttt gataagtaat ttcaccagcc
     4561 aacgtcactg ccgacgagac cgtaatatcg cttttcggtg gtacaaacgt attgggcaat
     4621 ttactccctt gtactttaat tcctgtcagt gtcaggtaat aaggcgaagg attgataact
     4681 tttagttgtt tgccctcata cttaaaccgc aacatatttc gtgcttttcc tggttgcaca
     4741 ggtagatttt ccgggcgata aaatagcttg attttgtttt gtaatgccaa ctgtaaggta
     4801 ttcttctgtg tatttttctc atctgttggc ggaattgctt taacattcat ccaatacaaa
     4861 gtttcacgat cggtcggcaa attagtttta ccaagaaaca tgatacgtaa tgaagcttct
     4921 ttacgcgttg gcaacataaa gaggggagga gtgataacaa aatcctggct ctttttttcc
     4981 gttttactgt catcaaccca tgattgaatt aaataactgg tgttgtcacc gctattttgc
     5041 acactgagtg ttatttgctt actttctgcc ggataaatca atcgcgttac gccaaggcca
     5101 aacccgcccg cggacgcatt attaataaat aatatcgata gtaaaaaaag aatccctgag
     5161 cgtataatat tttgtgatat ttttaattca tccctgaatc cataaaccca ttttttattt
     5221 ccaataatac atttcataca gcatccttgt tgatgaaata acacctgtta ttcatactct
     5281 attaaataat ttcaatctca ggttacaact ttttgtaacc tgaaatcgta tttatattta
     5341 ctcgtacagc accacaaaac ttgctgttga cttaactaca ccaggtgcaa cagtgctacc
     5401 gttttgagca taacctgcag taaaggccgc gctgaaatca tgggtcgtag catttcctgg
     5461 aattgtataa gcggcggtgg aggcgctacc ctctgccgca gtagtattca ggttaatcag
     5521 accattattc gtcgtacctt tggagtaaat tcgaatccct acaccagtcg cccccgcatt
     5581 gccgctgttg tcagcatccg ttgatccttc tacttcgtta gcgagcgttt tattagcgct
     5641 atctgcggta ccggtaaagc ggaactcaat tttctgatcc tctgtattgc tacattcggt
     5701 cagattaata ttgaaatcgg tctggttgct cgccccctgt gcttctatgt cgataccagt
     5761 gaaaaggttg gaaggcacaa ccggcaggac aacttcaatc tgttttgaat cggttgcgac
     5821 cttacaggta gctttcacta cacggccagt aaagcggatt ttccccccca ttgaatcaac
     5881 gttgtcatcc atatcagtga gcctgggata atcaacggtg ctccagtcag tatccgctcc
     5941 accaaattgt gctgcatcgc cgggtgctgc ggttaccgca gtagacatca gcatacctga
     6001 tgccagtaat actgcggata aattagataa attgaatttc atttatacct ttccttttgt
     6061 taaaaaatcc aagcaacatt ggtgttttta cattctgcgt taataagtta tatctcaca
//
LOCUS       DAPHCV010000180         6081 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.182, whole genome shotgun sequence.
ACCESSION   DAPHCV010000180 DAPHCV010000000
VERSION     DAPHCV010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.182"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(91..525)
                     /gene="merR"
                     /locus_tag="RH033_004368"
     CDS             complement(91..525)
                     /gene="merR"
                     /locus_tag="RH033_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hg(II)-responsive transcriptional regulator"
                     /protein_id="HDV2993457.1"
                     /translation="MENNLENLTIGVFAKAAGVNVETIRFYQRKGLLREPDKPYGSIR
                     RYGEADVVRVKFVKSAQRLGFSLDEIAELLRLDDGTHCEEASSLAEHKLKDVREKMAD
                     LARMETVLSELVCACHARKGNVSCPLIASLQGEAGLARSAMP"
     gene            597..947
                     /locus_tag="RH033_004369"
     CDS             597..947
                     /locus_tag="RH033_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric transport protein MerT"
                     /protein_id="HDV2993458.1"
                     /translation="MSEPQNGRGALFAGGLAAILASTCCLGPLVLVALGFSGAWIGNL
                     TVLEPYRPLFIGAALVALFFAWKRIYRPVQACKPGEVCAIPQVRATYKLIFWIVAVLV
                     LVALGFPYVVPFFY"
     gene            961..1236
                     /gene="merP"
                     /locus_tag="RH033_004370"
     CDS             961..1236
                     /gene="merP"
                     /locus_tag="RH033_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance system periplasmic binding
                     protein MerP"
                     /protein_id="HDV2993459.1"
                     /translation="MKKLFASLALAAAVAPVWAATQTVTLAVPGMTCAACPITVKKAL
                     SKVEGVSKVDVGFEKREAVVTFDDTKASVQKLTKATADAGYPSSVKQ"
     gene            1272..1694
                     /gene="merC"
                     /locus_tag="RH033_004371"
     CDS             1272..1694
                     /gene="merC"
                     /locus_tag="RH033_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial transporter MerC"
                     /protein_id="HDV2993460.1"
                     /translation="MGLMTRIADKTGALGSVVSAMGCAACFPALASFGAAIGLGFLSQ
                     YEGLFISRLLPLFAALAFLANALGWFSHRQWLRSLLGMIGPAIVFAATVWLLGNWWTA
                     NLMYVGLALMIGVSIWDFVSPAHRRCGPDGCELPAKRL"
     gene            1746..3440
                     /locus_tag="RH033_004372"
     CDS             1746..3440
                     /locus_tag="RH033_004372"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="HDV2993461.1"
                     /translation="MSTLKITGMTCDSCAVHVKDALEKVPGVQSADVSYAKGSAKLAI
                     EVGTSPDALTAAVAGLGYRATLADAPSVSTPGGLLDKMRDLLGRNDKTGSSGALHIAV
                     IGSGGAAMAAALKAVEQGARVTLIERGTIGGTCVNVGCVPSKIMIRAAHIAHLRRESP
                     FDGGIAATTPTIQRTALLAQQQARVDELRHAKYEGILEGNPAITVLHGSARFKDNRNL
                     IVQLNDGGERVVAFDRCLIATGASPAVPPIPGLKDTPYWTSTEALVSETIPKRLAVIG
                     SSVVALELAQAFARLGAKVTILARSTLFFREDPAIGEAVTAAFRMEGIEVREHTQASQ
                     VAYINGEGDGEFVLTTAHGELRADKLLVATGRAPNTRKLALDATGVTLTPQGAIVIDP
                     GMRTSVEHIYAAGDCTDQPQFVYVAAAAGTRAAINMTGGDAALNLTAMPAVVFTDPQV
                     ATVGYSEAEAHHDGIKTDSRTLTLDNVPRALANFDTRGFIKLVVEEGSGRLIGVQAVA
                     PEAGELIQTAALAIRNRMTVQELADQLFPYLTMVEGLKLAAQTFNKDVKQLSCCAG"
     gene            3458..3820
                     /gene="merD"
                     /locus_tag="RH033_004373"
     CDS             3458..3820
                     /gene="merD"
                     /locus_tag="RH033_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance co-regulator MerD"
                     /protein_id="HDV2993462.1"
                     /translation="MSAYTVSQLAHNAGVSVHIVRDYLVRGLLRPVACTTGGYGVFDD
                     AALQRLCFVRAAFEAGIGLDALARLCRALDAADGAQAAAQLAVLRQLVERRRAALAHL
                     DAQLASMPAERAHEEALP"
     gene            3817..4053
                     /gene="merE"
                     /locus_tag="RH033_004374"
     CDS             3817..4053
                     /gene="merE"
                     /locus_tag="RH033_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum mercury transporter MerE"
                     /protein_id="HDV2993463.1"
                     /translation="MNAPDKLPPETRQPVSGYLWGALAVLTCPCHLPILAAVLAGTTA
                     GAFLGEHWGVAALALTGLFVLAVTRLLRAFRGGS"
     gene            4089..4757
                     /locus_tag="RH033_004375"
     CDS             4089..4757
                     /locus_tag="RH033_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDV2993464.1"
                     /translation="MAQAAARGQLDLHYQPLVDLRDHRIAGAEALMRWRHPRLGLLPP
                     GQFLPLAESFGLMPEIGAWVLGEACRQMHKWQGPAWQPFRLAINVSASQVGPTFDDEV
                     KRVLADMALPAELLEIELTESVAFGNPALFASFDALRAIGVRFAADDFGTGYSCLQHL
                     KCCPITTLKIDQSFVARLPDDARDQTIVRAVIQLAHGLGMDVIFRRRLHQLIGRNGCC
                     AASS"
     gene            4832..>6081
                     /locus_tag="RH033_004376"
     CDS             4832..>6081
                     /locus_tag="RH033_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV2993465.1"
                     /translation="MATDTPRIPEQGVATLPDEAWERARRRAEIISPLAQSETVGHEA
                     ADMAAQALGLSRRQVYVLIRRARQGSGLVTDLVPGQSGGGKGKGRLPEPVERVIHELL
                     QKRFLTKQKRSLAAFHREVTQVCKAQKLRVPARNTVALRIASLDPRKVIRRREGQDAA
                     RDLQGVGGEPPAVTAPLEQVQIDHTVIDLIVVDDRDRQPIGRPYLTLAIDVFTRCVLG
                     MVVTLEAPSAVSVGLCLVHVACDKRPWLEGLNVEMDWQMSGKPLLLYLDNAAEFKSEA
                     LRRGCEQHGIRLDYRPLGQPHYGGIVERIIGTAMQMIHDELPGTTFSNPDQRGDYDSE
                     NKAALTLRELERWLTLAVGTYHGSVHNGLLQPPAARWAEAVARVGVPAVVTRATSFLV
                     DFLPILRRTLTRTGFVIDHIHYYA"
BASE COUNT         1091 a         1951 c         1938 g         1101 t
ORIGIN      
        1 cccggacgag tgggaatcca tggtagggat ttttaccgaa atggaagaac actattaggg
       61 ggcacctcag aaaacggaaa ataaagcacg ctaaggcata gctgaccttg ccaggcctgc
      121 ttcgccctgt agtgacgcga tcaacgggca ggaaacattc ccctttcgtg catggcaggc
      181 gcacacgagt tcagacagca cggtttccat gcgcgccaag tcggccatct tctcgcgcac
      241 gtccttgagc ttgtgttcgg ccaggctgct ggcctcctcg cagtgggtgc catcgtcgag
      301 ccgcaacagc tcggcaatct cgtccagact gaaccccagc cgctgtgccg atttcacgaa
      361 tttcacccga accacgtccg cctccccata gcggcggatg ctgccgtaag gcttgtccgg
      421 ttcccgcaac aggcccttgc gctgatagaa gcggattgtc tccacgttga ccccggccgc
      481 cttggcaaaa acgccaatgg tcaggttttc caaattattt tccatatcgc ttgactccgt
      541 acatgagtac ggaagtaagg ttacgctatc caatccaaat tcaaaagggc caacgtatgt
      601 ctgaaccaca aaacgggcgc ggtgcgctct tcgccggcgg gctggccgcc attcttgcat
      661 cgacctgctg cctggggccg ctagtactgg tcgccctggg cttctccggt gcttggatcg
      721 gcaacctgac ggtgctggaa ccctatcgac cgttgttcat cggcgcggcg ctagtggcgc
      781 tgttcttcgc ctggaagcgg atttaccggc ccgtgcaggc atgcaagcca ggtgaggtct
      841 gcgcgattcc gcaggtgcgc gccacctaca agctgatttt ctggatcgtg gccgtgctgg
      901 tcctggtcgc gcttggattt ccctatgtcg ttccattttt ctattaacca ggagttcatc
      961 atgaagaaac tgtttgcctc ccttgccctc gccgccgctg ttgccccggt gtgggccgct
     1021 acccagaccg tcacgctagc ggttcccggc atgacttgcg ccgcctgccc gatcacagtc
     1081 aagaaagcgc tctccaaggt cgaaggcgtg agcaaggtcg atgtgggctt cgagaagcgc
     1141 gaggccgtcg tcacttttga cgacaccaag gccagcgtac agaagctgac caaggccacc
     1201 gcagacgccg gctatccgtc cagcgtcaag cagtgagcca gcaagccaac gacaacagcg
     1261 agagccgctt catgggactg atgacacgca ttgccgataa aaccggcgcg ctcggcagcg
     1321 tcgtttccgc gatgggctgc gccgcctgct ttccagccct cgccagcttc ggcgcggcca
     1381 tcgggctggg cttcttgagc cagtacgagg gactgttcat cagccgcctg ctgccgctgt
     1441 ttgccgcgct ggccttcctg gcgaacgcgc tgggttggtt cagtcatcgg caatggctgc
     1501 gcagtctgct cggcatgatc ggcccggcca tcgtgtttgc ggccacggtc tggctgctcg
     1561 gcaactggtg gacggcgaac ctgatgtacg tcggcctggc cttgatgatt ggggtgtcga
     1621 tctgggactt cgtgtcgccg gcgcatcgcc gttgcggacc ggacggctgc gaactccccg
     1681 ccaagcgctt gtgaaagacg gctgaccgtg cgacacggcg gcccacacga ataaggaacg
     1741 atggtatgag cactctcaaa atcaccggca tgacttgcga ctcgtgcgca gtgcatgtca
     1801 aggacgccct ggagaaagtg cccggcgtgc aatcagcgga tgtctcctac gccaagggca
     1861 gcgccaagct cgccattgag gtcggcacgt cacccgacgc gctgacggcc gctgtagctg
     1921 gactcggtta tcgggccacg ctggccgatg ccccctcagt ttcgacgccg ggcggattgc
     1981 tcgacaagat gcgcgatctg ctgggcagaa acgacaagac gggtagcagc ggcgcattgc
     2041 atatcgccgt catcggcagc ggcggggccg cgatggcagc ggcgctgaag gccgtcgagc
     2101 aaggcgcacg tgtcacgctg atcgagcgcg gcaccatcgg cggcacctgc gtcaatgtcg
     2161 gttgtgtgcc gtccaagatc atgatccgcg ccgcccatat cgcccatctg cgccgggaaa
     2221 gcccgttcga tggcggcatc gccgctacca cgccgaccat ccagcgcacg gcgctgctgg
     2281 cccagcagca ggcccgcgtc gatgaactgc gccacgccaa gtacgaaggc atcttggagg
     2341 gcaatccggc gatcactgtg ctgcacggct ccgcccgctt taaggacaat cgcaacctga
     2401 tcgtgcaact caacgacggc ggcgagcgcg tggtggcatt cgaccgctgc ctgatcgcca
     2461 ccggcgcgag cccggccgtg ccgccgattc ccggcctgaa agacactccg tactggactt
     2521 ccactgaagc gctggtcagc gagacgattc ctaagcgcct ggccgtgatt ggctcatcag
     2581 tggtggcgct ggagctggcg caggcgttcg cccgactcgg agcgaaggtg acgatcctgg
     2641 ctcgcagcac gctgttcttc cgcgaagacc cagctatagg cgaagccgtc acggccgcat
     2701 tccgcatgga gggcatcgag gtgagggaac acacccaggc cagccaggtc gcgtatatca
     2761 atggtgaagg ggacggcgaa ttcgtgctca ccacggcgca cggcgaactg cgcgccgaca
     2821 agctgctggt cgccaccggc cgcgcgccca acacacgcaa gctggcactg gatgcgacgg
     2881 gcgtcacgct caccccgcaa ggcgctatcg tcatcgaccc cggcatgcgt acaagcgtgg
     2941 aacacatcta cgccgcaggc gactgcaccg accagccgca gttcgtctat gtggcggcag
     3001 cggccggcac tcgcgccgcg atcaacatga ccggcggtga cgcggccctg aacctgaccg
     3061 cgatgccggc cgtggtgttc accgacccgc aagtggcgac cgtaggctac agcgaggcgg
     3121 aagcgcacca tgacggcatc aaaactgata gtcgcacgct aacgctggac aacgtgccgc
     3181 gcgcgctcgc caacttcgac acgcgcggct tcatcaaact ggtggttgaa gaaggcagcg
     3241 gacgactgat cggcgtgcag gcagtggccc cggaagcggg cgaactgatc cagacggccg
     3301 cactggcgat tcgcaaccgg atgacggtgc aggaactggc cgaccagttg ttcccctacc
     3361 tgacgatggt cgaagggttg aagctcgcgg cgcagacctt caacaaggat gtgaagcagc
     3421 tttcctgctg cgccgggtga ggacaaggag gtgtgcgatg agcgcctaca cggtatcgca
     3481 actggcccat aacgctgggg tgagcgtaca tatcgtgcgc gactacctgg tgcgcggctt
     3541 gttacggccg gtggcctgca ccacgggcgg ctacggcgtg ttcgacgatg cggccttgca
     3601 acggctgtgc ttcgtgcgcg cggccttcga ggcgggtatc ggcctggatg ccctggcgcg
     3661 gctgtgccgt gcgctcgacg cagcggacgg cgcacaagcc gcagcgcagc ttgccgtgct
     3721 gcgccagttg gtcgagcggc ggcgcgcggc gttggcccat ctggacgcgc aactggcctc
     3781 catgccagcc gagcgggcgc acgaggaggc attgccgtga acgcccctga caaactgccg
     3841 cccgagacgc gccaacccgt ttccggctac ctgtggggtg cgctggccgt gttgacctgc
     3901 ccctgccatc tgccgattct cgccgccgtg ctggccggga cgaccgccgg tgccttcctt
     3961 ggcgagcatt ggggtgttgc cgcgctcgcg ctgaccggct tgttcgttct ggccgtaacg
     4021 cggctgctgc gcgccttccg gggcggatca tgacgagttc gcagcccgcc ggatggacgg
     4081 cggccgagtt ggcgcaggcg gcggcgcgcg gacagcttga cctgcattac cagccgctgg
     4141 tcgatctgcg cgatcaccgg atcgctggcg cggaagcgtt gatgcgctgg cggcatccga
     4201 ggcttggcct gttgccgccc ggccagttcc tgccgctggc cgagtcgttc ggcctgatgc
     4261 cggaaatagg cgcgtgggtg ctgggcgagg cctgtcgcca gatgcacaag tggcaaggac
     4321 cggcatggca accgttccgt cttgccatca atgtgtccgc cagccaggtt gggccaacgt
     4381 tcgacgacga ggtaaagcgg gtgctggccg atatggccct gcccgccgag cttctggaga
     4441 tcgaactgac cgaatcggtc gcattcggca atccagccct gttcgccagt ttcgacgcct
     4501 tgcgcgccat cggcgtgcgc ttcgccgccg acgacttcgg caccggctat tcctgcctgc
     4561 aacatctgaa atgctgcccc atcaccacat tgaaaatcga ccaatccttt gtcgccaggc
     4621 tcccggatga tgcccgtgac caaactatcg tgcgggcggt gatccagctc gcgcacgggc
     4681 tgggcatgga tgtcattttc agaagacgac tgcaccagtt gattgggcgt aatggctgtt
     4741 gtgcagccag ctcctgacag ttcaatatca gaagtgatct gcaccaatct cgactatgct
     4801 caatactcgt gtgcaccaaa gcgaggtgag catggcgacg gacaccccac ggattccaga
     4861 acaaggcgtg gccactctgc ctgatgaggc ttgggagcgt gcgcgccgtc gtgcggagat
     4921 catcagtccg ttggcgcagt cggagacggt cgggcacgaa gcggccgata tggcggctca
     4981 ggcgctgggc ttgtctcggc gccaggtata cgttctgatc cggcgtgccc ggcaaggcag
     5041 cggcctcgtg acggatctgg tgcccggcca gtccggtgga ggtaaaggta aggggcgctt
     5101 gccggaaccg gtcgagcgcg tcatccacga gctactgcaa aagcggttcc tgaccaagca
     5161 gaagcgcagc ctagcggcct ttcaccgcga agtcactcag gtgtgcaagg ctcaaaaact
     5221 gcgagtgccg gcgcgcaata ccgtggcctt acggatcgct agccttgacc cgcgcaaggt
     5281 catccgccgg cgggaaggcc aggatgccgc tcgtgaccta caaggtgtgg gcggcgagcc
     5341 tcctgccgtg accgcgccgc tggagcaggt gcagatagac catacggtca tcgacctgat
     5401 cgtggtcgat gaccgcgacc ggcaacctat tggccgcccg tacctgaccc tcgccatcga
     5461 cgtgttcacc cgctgcgtgc tcggcatggt cgtcacgctg gaagcgccgt ctgccgtttc
     5521 ggttggcctg tgcctcgtgc atgtcgcctg cgacaagcgc ccttggctgg aaggactgaa
     5581 cgtggaaatg gattggcaga tgagcggcaa gcccttgctg ctctacctag acaacgcggc
     5641 cgagttcaag agcgaggccc tgcgccgggg ttgcgagcag catggcatcc ggctggacta
     5701 tcgcccgctg ggacagccgc actatggcgg catcgtggaa cggatcatcg gcacggcgat
     5761 gcagatgatt cacgacgaac tgccgggaac gaccttctcc aaccctgacc agcgcggcga
     5821 ctacgattcc gaaaacaagg ccgccctgac gctgcgcgag ctagagcgct ggctcacatt
     5881 ggcggtcggc acctaccacg gttcggtgca caacggcctg ctccaaccgc cggccgcgcg
     5941 ctgggccgag gccgtggcgc gtgtcggcgt accggccgtc gtcacacgcg ctacttcgtt
     6001 cctggtcgat tttctgccga tcctccggcg cacgctgacc cgcaccggct ttgtcatcga
     6061 ccacatccac tactacgccg a
//
LOCUS       DAPHCV010000181         6024 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.183, whole genome shotgun sequence.
ACCESSION   DAPHCV010000181 DAPHCV010000000
VERSION     DAPHCV010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.183"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            931..2193
                     /gene="trbA"
                     /locus_tag="RH033_004377"
     CDS             931..2193
                     /gene="trbA"
                     /locus_tag="RH033_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HDV2993466.1"
                     /translation="MSYQQTSAAEDPMAIWYIVGAICLLFAIIIWRFLPEIVFASCLI
                     LHTLWGMIDWGPFHNFAAPRYNLLAITANNAATITFSQWLDVMSRTVGILWLILLPMT
                     FGFLWMWFHHPAQPRFTRRPLNIHTLPHIFSALSPAIAPVLADGDNNRLFHGQKRPER
                     RVALTPEAFVEQNNLIRNMQLDVASTRQCFMAQLGQPLTSWKDMAPHEKALFAIFGLQ
                     FFLGDRKAAVALMNNLNLSCRLKSKRDQGRFSTPVYSLARNAFIRVIKTEDAQQWLRQ
                     HRYVRSGLVWLYAHDLRLTPPNWLWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     ENEACRLGLPCPEPCVEEAIEGLRQDMLGLGLIWDEPQPDRDRRRQIRTRWSLTDDEI
                     PHRHDNDEGSDTGETTETRHPADKEKAQ"
     gene            2190..3314
                     /locus_tag="RH033_004378"
     CDS             2190..3314
                     /locus_tag="RH033_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HDV2993467.1"
                     /translation="MTIRYTATLNNKHLSLTALYPDGSREVIAILPVTELGALINRNN
                     RLMHLSFSSPFREITETVLVDTVDAERPGHHIGRMLSAIDACLDAEYAGKGKHPSRKY
                     GFWRTLMCGFIAGIALPATVAGYHWMKAEPPSTLAYPTSQEWRTEPVTDTETPRPHTE
                     HHVNSIPARPAPTQQEPSSAPAPTAVTEEQHVQAAEARRNLATVLKRNADRGMFTINL
                     STGHERTLYAFLDPACINCRLLEPALKQLAREFSVVIYPVSVIGGEASTNRTAPLLCE
                     PDAQKRATGWHQLYSADGGMAPTGDAPAAPDEKCLTAARAAIDVNNQAFRQFGFGGTP
                     WVLSDTGWHLPSGILKDATSVRLFLKATDTEEYHNEPAPR"
     gene            3295..5598
                     /locus_tag="RH033_004379"
     CDS             3295..5598
                     /locus_tag="RH033_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HDV2993468.1"
                     /translation="MSQHHVNPDLIHRTAWGNPLWNALHNLNITGLCLAGSIITALIW
                     PLALPVCLLFTLVTSVIFMLQRWRCPLRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQY
                     EADETSPARGIFYVGYRRINDIGRELWLSMDDLTRHVMFFATTGGGKTETTFAWLLNP
                     LCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEIIQSGEKSR
                     PQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVRERK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTS
                     AHTTSALGRMFVTQQSMLLARDLGYRLEGTDAQTLEVKKYKGSFPYICILDEVGAYYT
                     DRIAVEATQVRSLEFSLIMTAQDQERIEGQTSATNTATLMQNAGTKFAGRIVSDDKTA
                     RTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLNAGEFFT
                     VFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPE
                     HVSSIIGVLTAKPSRKRRKNRTEPYRIIDTFQRRLATAQTSLDLLPQYDTDIESRANE
                     LWKKAVHTINNTTREERRVCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEMLLPVPD
                     LTASPPHKKNIAQTPSGGKQESRKKRF"
BASE COUNT         1540 a         1720 c         1453 g         1311 t
ORIGIN      
        1 aaccgctaaa atcaacttct gtgctaagcg cattctgatg cgtaatcaaa tgagcaaaat
       61 aatcagacat acagacacag acgaaagact cttccgtctg agtaaacacc atcatttata
      121 tgagaaaaag aaaacaaaag agggatgaca atcccatgat atgggaaaaa cttgccatgc
      181 ttactgcagg tgcatttttg atactggcag tcctgtgggg gtaataatat cctgtttatt
      241 aaagggcaga aggcggtgcc cctctgccct taagtacgtt tccgggatta tcagtcaata
      301 agttcccgga tcagttccag aagagctatc actgcattga ccacagcaac aactgccctg
      361 agaacgatga ttaacaggtt aatcatccgt ttctccgtca atggaggaaa tcactgctgc
      421 ttactcttac attgcctgtc acaacagctc tctcattgtt agctgaaaag agacttcctc
      481 cggccagagc accagactca gcaccacctg tttgtccggc caggtctttg aatagcacag
      541 atccatagtc ccgatggatt gatgcgaagg taagtctcca gacaaggcta acttactgat
      601 tatacttgtt tcaggcgata tcgctactcc cggaataata gattgtaact gacagattgc
      661 gatcaggtga atataacgct attctggcaa cgttagttga aaagagactt cctccggtca
      721 gagccaccaa ctccaccccg gaatcgcaaa aaaaccggcc tcaccagtcg gtttttttgt
      781 acctgtctcc caccaccttc tgccgttcgc tcttctctgt aaaaatgtcc tgttgaattc
      841 atccctgtcc ggttattacc ggcgctgatt cgcattcatg ccacccgact taatcccgaa
      901 atttatccat tcatctatgg aggaatcccc gtgtcttacc aacagacgtc tgccgccgaa
      961 gatccaatgg ctatatggta tatcgtcggt gccatatgcc tgctctttgc gattatcatc
     1021 tggcgctttt tacctgaaat cgtcttcgcc tcctgcctga tcctgcacac actgtggggc
     1081 atgattgact gggggccctt tcacaacttt gccgcccccc gttacaacct gctggccatc
     1141 actgctaaca acgcggcaac catcactttc agccagtggc tggatgttat gtcccggaca
     1201 gtggggatcc tctggttgat tctgcttccc atgacattcg gctttctgtg gatgtggttt
     1261 caccatccgg cccagccccg ttttacccgc aggccactga atatccacac actgccccat
     1321 attttttcag ctctgtcacc ggcgatcgcc cccgttctgg ctgatggaga caacaacaga
     1381 ctcttccacg gacaaaaacg tcccgagcgc cgtgtcgcac taacccccga agcctttgtg
     1441 gagcagaaca accttatccg caacatgcag ctcgatgttg cgtccacacg ccagtgcttc
     1501 atggcgcaac tggggcaacc actgaccagc tggaaagaca tggccccaca tgaaaaagcg
     1561 ctctttgcca tctttggcct gcagttcttc ctcggtgacc ggaaagcagc ggtagccctg
     1621 atgaacaacc tcaatctctc ctgcaggctc aaaagtaaac gggaccaggg gcgtttcagc
     1681 acgccggtgt attcactggc cagaaacgcc tttatccggg tcataaaaac agaagacgca
     1741 caacagtggc tcaggcagca ccgttacgtt cgcagcggtc tggtctggct ttacgcacat
     1801 gatttgcgcc tgacgccccc caactggctg tggctcaagg gcgttgaccg gacgctcttc
     1861 tatgcccttc accgggcaaa caccacaaaa ggtttcatcg aaggggccgg cgtggtggcc
     1921 gttgcccggg cagaaaatga agcatgtcgt ctcggcctcc cctgcccgga accctgcgtg
     1981 gaagaagcca ttgaagggct gcgccaggac atgctgggac tggggctgat atgggatgaa
     2041 cctcagccag accgcgatcg cagacgccaa atcaggaccc gctggtcact cacggatgac
     2101 gagataccgc accgccatga taacgacgaa ggctcagaca ctggtgaaac caccgaaacc
     2161 cggcatcccg ccgacaagga aaaagcacaa tgaccatccg ctacactgca acactgaaca
     2221 ataaacacct gtccctgacc gccctgtacc ctgacggtag tcgggaggtt attgccatcc
     2281 tgccggtaac agaactgggc gcactgatta accgtaataa caggctgatg cacctgtcct
     2341 tcagctcccc ttttcgcgaa ataaccgaaa cggtgcttgt tgataccgtt gatgcagaac
     2401 gtcccggaca tcacatcggc agaatgctct cagcgattga tgcctgcctg gatgccgaat
     2461 atgccggaaa aggaaaacat ccctcccgga aatatggctt ctggcggaca ctgatgtgtg
     2521 gctttatcgc cggaattgct ctgccggcga cagttgcagg ttaccactgg atgaaggcag
     2581 agcccccttc cacgctggct taccctacat ctcaggaatg gagaactgaa ccagtaaccg
     2641 acacagagac accacgccca catacggaac atcatgttaa cagcatcccg gcccgcccgg
     2701 ctcccacaca gcaggaacct tcttctgccc ccgccccgac ggcagtgacg gaagaacaac
     2761 acgttcaggc cgctgaggcc cgccgtaatc tggccacagt acttaaacgc aacgcagacc
     2821 gggggatgtt caccattaac ctgtccactg gtcatgaacg cacgctgtac gccttccttg
     2881 accccgcatg catcaactgc cgtctgctgg aacccgcgct taagcaactg gcccgggaat
     2941 tcagcgtggt gatttatccg gtctccgtaa tcggcggaga ggccagcaca aaccggactg
     3001 cgccactgct gtgtgaaccg gacgcacaaa aacgggcgac cggatggcat cagctgtatt
     3061 ccgcagacgg tggtatggca cccaccggcg atgcaccggc tgcaccggat gaaaagtgtc
     3121 tgaccgctgc tcgtgctgcc atcgatgtca ataaccaggc tttccgccag ttcggttttg
     3181 gcggcacccc atgggtgtta tccgataccg gctggcacct gccgtccggc atcctgaaag
     3241 acgccacttc cgttcgcctc ttcctgaaag ccacagatac cgaggaatat cacaatgagc
     3301 cagcaccacg ttaatcctga cctcatacac cgcacggcct ggggaaaccc gttgtggaat
     3361 gcactgcaca atctgaacat cactggtctg tgtctcgccg gcagtatcat tacggccctg
     3421 atatggccac tggcccttcc tgtctgcctg ctgttcaccc tggtcacctc cgtgatattc
     3481 atgctgcaac gctggcgatg ccccctgcgt atgcccatga cactcagcct tgatgaccct
     3541 tcccaggaca gaaaggtcag gcgaagcctc ttcagcttct ggcccaccct gtttcagtat
     3601 gaagcagatg aaacatcccc cgcacggggg attttttatg tcggttaccg ccgtatcaat
     3661 gatatcggtc gtgagctctg gctgagcatg gatgacctca cccggcatgt catgtttttt
     3721 gccaccaccg gtggcggtaa aacagaaacc acctttgcct ggctgctcaa cccgctatgc
     3781 tggggaagag gctttacctt tgttgacgga aaggcacaga acgataccac gaggacaatc
     3841 tggtatctct cccgacgttt tggcagggag gatgatatcg aggtcatcaa cttcatgaac
     3901 ggcggaaaat cccgcagtga aatcattcag agcggcgaaa aatcccgccc acagtcaaac
     3961 acctggaacc cgttcgcatt cagcaccgaa gccttcaccg ccgaaaccat gcagtccatg
     4021 cttccccaga acgttcaggg gggagaatgg cagtcacggg cgatagccat gaacaaagcc
     4081 ctggtttttg gcacaaaatt ctggtgcgtc cgggaaagaa aaaccatgtc cctgcagatg
     4141 ttacgggagc atatgacgct ggaaggtatg gcaaaactct attgccgtgg gcttgatgac
     4201 cagtggccgg aagaagccat cgcaccactg agaaattacc tgcaggacgt tccgggattc
     4261 gatatgtcac tggtgagaac gccgtcagca tggactgagg agccccgtaa acagcatgct
     4321 tatctctccg gtcagttctc ggaaacattc acaacctttg ccgaaacctt cggggacgtc
     4381 tttgcggcag atgccggcga catcgatatt cgcgacagta ttcacagcga ccgcattctg
     4441 attgtgatga tccctgcact ggacacatcg gctcatacca cgtcggcact cggtcgtatg
     4501 ttcgtcaccc agcagagcat gctcctcgcc cgtgacctgg gctaccggct ggaaggcaca
     4561 gatgcacaga ccctggaagt caaaaaatac aaaggcagct tcccctatat ctgtatcctt
     4621 gacgaagtcg gtgcctacta cacagaccgc attgctgtgg aggccaccca ggtacgttca
     4681 ctggagtttt ccctgataat gacggcgcag gatcaggagc gaattgaagg gcagacctcc
     4741 gccaccaaca ccgccacact gatgcagaac gccggcacca aattcgccgg acgcattgtc
     4801 agtgacgaca aaacggcaag aacggtgaaa aatgccgccg gtgaagaggc acgggccaga
     4861 atgggcagcc tgcagcgcca cgatggtgtg atgggtgagt cctgggtgga cggtaaccag
     4921 atcaccatcc agatggaaag caaaatcgac gtgcaggatt tgatcagact gaatgctggt
     4981 gagttcttta ccgttttcca gggggatgtg gtgccgtcag cctctgtgta tattccggac
     5041 agcgagaaaa gttgcgacag tgatccggtg gtcatcaacc gctatatttc cgttgaagcc
     5101 ccccgcctgg agcgtctccg ccgtctcgtc cccaggacag tgcagcgcag gctccccaca
     5161 ccggagcatg tcagcagcat catcggtgta ctgacagcaa aaccatcccg taaacgtcgt
     5221 aaaaaccgta cagagcctta ccgtatcatt gatacattcc agcgccggtt ggcaacagcg
     5281 cagacatcac tggatctgct tccgcaatat gatacagata tcgaatcccg ggcaaacgaa
     5341 ctgtggaaaa aggctgtgca cactatcaat aacacaacca gggaagaacg ccgggtatgt
     5401 tatatcacac tgaatcgtcc ggaagaggaa cactccggtc ctgaagatat accttcagtg
     5461 aaggcgattc tgaatactct gctccccctg gagatgctgt tacctgtacc ggatctaact
     5521 gcatccccgc cccataaaaa aaatatcgct cagacaccat caggtggaaa acaagagagt
     5581 cgtaaaaaaa gattctgata acacctgtat ggctgatttt aataacaacc ttaataacaa
     5641 cctttacgcg attattaatc tcctccgctc accatagtaa gcggaggatt caggatttca
     5701 atccatcatc agaggtacaa taaatgtgta ttagtagccc ggaaacaaac tcatggtctg
     5761 ttatttatcg taaaaattcc ggagaagata tcaatatcac ttccctgacg tttaaaaata
     5821 gtcttctggc agcccggata ctgatggtgc ccgaaaacta tatgatatgc atactcagaa
     5881 acggggagag ggtcagacga tgggacagag aaatacttgc aggctcaaac agatggtaca
     5941 agtgttctcc cgataatttc gaaatactgg gtaaactccc cataataaat aaagtcacaa
     6001 cactgataaa atcataatct actg
//
LOCUS       DAPHCV010000182         6013 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.184, whole genome shotgun sequence.
ACCESSION   DAPHCV010000182 DAPHCV010000000
VERSION     DAPHCV010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.184"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            27..350
                     /locus_tag="RH033_004380"
     CDS             27..350
                     /locus_tag="RH033_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HDV2993469.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGLTEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSDPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            343..618
                     /locus_tag="RH033_004381"
     CDS             343..618
                     /locus_tag="RH033_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HDV2993470.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMTVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFNGKPMIFIE"
     gene            630..1220
                     /locus_tag="RH033_004382"
     CDS             630..1220
                     /locus_tag="RH033_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV2993471.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFIQQLANGRWHVLRRLPEARFATGHDHQGRPRKNRLPVEVVKIPLSG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRLWLTR"
     gene            1217..1618
                     /locus_tag="RH033_004383"
     CDS             1217..1618
                     /locus_tag="RH033_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV2993472.1"
                     /translation="MNRHTQIRQVVLARLREQCGDSATFFDGLPAFVDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            1629..2372
                     /locus_tag="RH033_004384"
     CDS             1629..2372
                     /locus_tag="RH033_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV2993473.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASASVKVGATTTLTFTVKPDGASDKAISVHSTDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTSDGDFVAVATVAVSAAG"
     gene            2433..2819
                     /locus_tag="RH033_004385"
     CDS             2433..2819
                     /locus_tag="RH033_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HDV2993474.1"
                     /translation="MFLKQGTFNYEKQSVVLSELSGLQRIEYLAFVQQRTAKFDAGEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNADQSKDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGAIDNAGDDEHEALTPEKS"
     gene            2828..3157
                     /locus_tag="RH033_004386"
     CDS             2828..3157
                     /locus_tag="RH033_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HDV2993475.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            3129..>6013
                     /locus_tag="RH033_004387"
     CDS             3129..>6013
                     /locus_tag="RH033_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDV2993476.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTTQAASSAATAASVHAQTVEKNAQVHERMAREVEKTRQRMEALSQK
                     MRDEQAQAMALAEAQDKAAAAFYRQIDSVKQASAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALIAEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRQNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMLGGVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGDQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQIAALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNDLQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINSMQYADQAVRDAAIQRENERYEKALASGKKKTRETRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSAGIATEKMTEAHKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNDLKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEVVQGNWKNGVLRAFQDFTVDADNTAGTAEQVFSSAFSNMGNGLATFVTT
                     GKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQSADLS
                     RYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIG"
BASE COUNT         1502 a         1361 c         1881 g         1269 t
ORIGIN      
        1 ctgaacgggg ctgtgcggag acagttatgg caaagaattt tgtagaagaa ggaaaaacgg
       61 tggcgattgt tgccagtgca gccatcagca gcggagatct ggtgcaggtg ggtgatgttt
      121 ttgcggtagc gctgaccgat attccacagg gtgaaacagg cgacggcctg accgaaggtg
      181 tgtttatgct gcctaagctg aaaacggatg acatgaaaac gggtaagaag gtttatctga
      241 agtccggaaa agttcagttg actaacagcg gctctgatcc gctggtcggg gttgtctggg
      301 cagatgccgg aaccagtgca gaagaagtgc cggtaaaact caatgtctga tcccttttcc
      361 cggctggcag cgcgtatgga tgcgatcacg gtcagaaaga tgggaaagac agcctcgatt
      421 aatgatgtcg atatgactgt gatcccggga gaaacactgg cagagctgaa tgctctgtcc
      481 ggacctgcgg tctctctggt ggtgttttct tcgggatacc gcccacggcg cggggatcgc
      541 gttgtttatg acggacaaca atggacggtc acacggcatg aacgctttaa cggtaagcca
      601 atgatcttta ttgagtaaag aggtgtggga tgaaggggct tgagaatgcc atccgcaatc
      661 tgaacagcct tgatacccgt atggtgccac aggccagcgc atgggcgata aaccgtgtgg
      721 cacagaaagc ggtctcggtt gccacccggc aggttgccgg gaataccgtt gcgggagata
      781 accaggtgaa agggatcccc ctgaaactgg tacgtcagcg tgtccgggtg tttaaagcca
      841 gtccgtcagg aaaaatgacg gccaggattc gcgttaaccg gggcaatctg cccgccatca
      901 aactgaacac aacacggcgg cgtgctggtg aaggattgag agtgggaaaa tactttttcc
      961 ggggggcatt tattcagcaa ctggcgaatg gccgctggca tgttctgagg cgtcttcctg
     1021 aagcgcgttt tgcaacaggg catgaccatc agggcaggcc aagaaaaaat cgtcttcctg
     1081 tggaggtagt gaaaatcccg ctgtccggac cgctgacaca ggcatttgaa gatgcccgcg
     1141 accgcatcat tgctgcggaa atgccgaaac agctggggta tgcactgaaa caacaactga
     1201 ggttatggct gacccgatga atcgacatac acaaatccgc caggtcgtac tggcacgcct
     1261 tcgggaacag tgtggagaca gcgccacgtt ttttgacggg cttccggcat ttgttgatgc
     1321 gcaggaactg cctgccgtgg cggtgtggct gagtgatgct cagtacaccg gaaaaatgac
     1381 ggatgaagat gactggcagg ctgttctgca tattgctgtc ttcatccggg cacaggcacc
     1441 ggattcagag ctggatatgt ggatggagag caccattttc ccggccctga atgatgtacc
     1501 ggcactttcc ggactcatcg acaccctgat cccactcggt tttaactatc aacgtgataa
     1561 tgagatggca acctgggcga tggcggaaat cacgtaccag atcacgtaca cgaattaagg
     1621 aggtggtaat gaccacacca aatccactgg caaaaacgaa aggtgcggga acgacgttct
     1681 ggatgtatac cggcaacggc gatgcgtttg cgaacccttt gtcggacact gactggctgc
     1741 gtcttgcgat ggtgaaggat ctgcaacctg gcgaaatgac cgctgatgca gaagatgaca
     1801 cttatctcga tgatgaagat gcagactgga aaacgacaac ccaggggcag aaatccgtcg
     1861 gtgatacttc ggcgacgctg gcctggcgtc cgggggacag cgggcagaaa aaactggttc
     1921 agttgttcga ctccggtgaa gtctgcgcgt ttcgtatcaa atatcccaac ggtactgttg
     1981 atgttttccg tggctggctg agttcactgg gtaaaaccat tgcctcaaaa gacgtgatga
     2041 cccgcaccgt gaaaatcagc ggtgtggggc gtccgtatct ggcagaggaa ggcactgaaa
     2101 ccgtgggcgt taccgggctg acggtggcac cggcatctgc cagtgtaaaa gtgggagcaa
     2161 ccaccacgct gacctttaca gtaaaacctg acggagccag tgacaaagcg atcagtgtgc
     2221 attcgacaga tccacagact gccacggtga ccctgaacgg gcttgtggcc acggtgaaag
     2281 gcgtgaagca gggcagtgtc agcattgtgg gcatgacttc tgacggcgat tttgtggcag
     2341 tggctacggt ggctgtcagc gccgcaggtt aacaggacga tactcatcat ttgccccggt
     2401 tatccggggc ttttttgcag gtggagaaca tgatgtttct gaaacagggc acgtttaatt
     2461 atgaaaaaca gtccgtggtg ctcagtgagc tgtccgggct gcagagaatt gaatatctgg
     2521 cgtttgttca gcagcgaacg gcaaagtttg atgccgggga gggagaactg ccggaggctg
     2581 aacgacagat tgcttttctg cggatgggga tggatatcaa tgcctggctg gtttcccgct
     2641 cactgtggaa tgcggatcag tctaaggatg tagagacgct ttgcgcatcc gttattacaa
     2701 catggtcgta tgatgccctg ggtgcggggg cggagatggt tctgtcgctg agcggtatgg
     2761 gggccattga taatgccggg gatgatgagc atgaggcgct gacgccggaa aagtcctgac
     2821 gcgggaaatg cagtttgtca tgcggcttgc ccgggagttc cggcgggcag actggcggcg
     2881 gatgctgtcg gaaatgtcgg ccactgagct tggtgagtgg ggcgattatt tccggatgca
     2941 gagcttcagt gatgtgtgga tggatgcgca gtttgcctcg ctgaaggcat tgatcgtgag
     3001 aatggtgtcc ggcagcagtg atgctgcggt ggctgatttc agccttttac cggaagagaa
     3061 cgggataccg gagcgaacgg acgaagaact gatgcatctt ggagaaggta tttccggagg
     3121 tgtgcgttat ggaccagata gccaacctgg tcattgattt ggggattgat gcggcagagt
     3181 ttaaaaatga aattccccgt atcaaaaacc ttctgaatgg tgccgccagc gatgcagaac
     3241 ggtcttctgc ccgtatgcag cgttttatgg agcgtcagac tcaggccgcc cggcagacaa
     3301 cgcaggcggc ttcttcggct gcaacagccg cctccgtcca tgcgcagacg gtggagaaga
     3361 acgcacaggt tcatgaacgc atggcccgcg aggtggagaa aacccgccag cgcatggagg
     3421 cgctgagcca gaaaatgcgc gatgaacagg cgcaggccat ggctctggcg gaggctcagg
     3481 ataaagcggc tgctgcgttt tatcgtcaga ttgacagtgt gaaacaggcc agtgcggggc
     3541 tgcaggaatt acagcgtatt cagcagcaga tccgacaggc cagaaacagt ggcgggattg
     3601 gtcagcagga ttatctggcg ctgattgctg aggttacggc gaaaacccgt gttcttacac
     3661 aggctgagga agaggctacc cgacagaaag tggcgtttat ccgtcagctt aaagagcagg
     3721 caacccgcca gaatctttct tcttctgagt tgcttcgtgc taaggctgcc cagctggggg
     3781 taagcagtgc tgcagaagtg tatatccgca aaatggagca ggcaggaaaa gccacgcatt
     3841 cgctgggtct gaaaagtgca gcagcccgcc aggagatagg cgttctgata ggtgaactgg
     3901 cccgcggcaa tttaggggcg ctgaggggat ccgggataac gctggctaac cgtgccggat
     3961 ggatagacac actgatgtca ccgaaaggca tgatgcttgg cggggttatt ggcggtattg
     4021 ccgcggccgt ctatggtctg ggtaaagcct ggtatgacgg tcagaaggag ggggaagaat
     4081 ttaaccgcca gttgtcgctg acggggcatt atgccggagt cactgccggg cagctgtgga
     4141 cgctcagtcg tgctatttcc gggaatggta tcacgcaaca tgctgcagcc ggtgcgctgg
     4201 cgcaggtggt ggggagtggt gcatttcgtg gaaacgatat cggtatggtg gcgagagctg
     4261 ccgcacagat ggagcgatcg gttggccagt cggtcagcga taccataaat cagtttaagc
     4321 ggctgaagga tgatcctgta aatgccgcga aggctctgga caatgagctg cattttctta
     4381 ctgccactca gcttgagcag atacgtgtcc ttggggatca gggacggtcc agtgatgctg
     4441 cacggatagc catgtctgca ctggcagagg aaaccggtcg gcgtactgcg gatattgata
     4501 ataacctcaa tgcgctgggc agtacgctga agtatctgtc tgatttgtgg agtcgtttct
     4561 gggatgcggc catgaatatt ggtcgtgaag actcgctgga tgaacagatt gccgctttac
     4621 aggagaaagt gtcgcgggcg aaaagactcc cctggacggc atcatcttct caggttgaat
     4681 acgatcagca gcgtcttaac gatcttcagg agaaaaaacg ccagaaggat ttgcaggatg
     4741 caaaagagca ggcagagcgg aattatcagg agcaacagaa acgacgtaat gctgaaaatg
     4801 ctgcactgaa ccggatgaat gaaacggaag cagcacgaca tcagcgtgaa attgcgcgta
     4861 ttaattccat gcagtacgcc gatcaggctg tcagggatgc agcgatacaa cgtgaaaatg
     4921 aacgttacga gaaagccctg gcatccggta agaaaaaaac acgcgaaacc cgtaatgatg
     4981 aggccacccg gttattgctg cagtacagtc agcaacaggc acaggtggaa ggacagattg
     5041 ctgctgcaag acagtcagca ggcattgcca ctgaaaagat gacagaagcg cataaacagc
     5101 ttctggctct gcagcagcgc atcagcgatc tggacgggaa aaaactgacg gcagatgaaa
     5161 agagtgtgct ggcccgtaaa gatgaactga ttcaggcact gacgctgctg gatgtaaaac
     5221 agcaggagct tcagaaacag acggcactca acgatctgaa gaaaaaaaca attcagctga
     5281 ccagtcaact ggctgaagaa gagcgcgctc agcgtcagca acatgacctg gatatcgcca
     5341 cggtgggtat gggtgatcag cagcggcagc gatatcaggt acaactgagt cttcgccaga
     5401 aataccagca acagctggag cagttgaggc gggatagtga gcagaaaggg acatataaca
     5461 cggatgacta cagaaaggcc gagcaggcgc tgacggagag cctgaaccga caactgaatg
     5521 agaatcgccg ttactggcaa cagcttgaag ttgtgcaggg taactggaaa aacggagtcc
     5581 tgcgtgcatt tcaggatttt accgtggatg cagacaatac ggcaggaaca gcagaacagg
     5641 tgttctcgtc agccttcagc aacatgggaa atggcctggc aacttttgtc actaccggca
     5701 aactcaactt caaatccttc acctcttcgg tgctgtcaga tatggcgaaa atcctggcgc
     5761 aggcaaccat gatgaaatcg ataaaaggga ttggcagtgt actgggattt gatctcagca
     5821 gcctttccct gaatgccaat ggggggattt atcagtctgc tgatttgagt cgttacagtg
     5881 gcacggtggt taaccgtccg acgttttttg cttttgcaaa aggcgcgggt gtgatggggg
     5941 aagctggacc tgaagccatt ctgccactgc gtcgtggtgc tgacggtaag ctgggggttg
     6001 tggcggatat tgg
//
LOCUS       DAPHCV010000183         6007 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.185, whole genome shotgun sequence.
ACCESSION   DAPHCV010000183 DAPHCV010000000
VERSION     DAPHCV010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.185"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..362
                     /locus_tag="RH033_004388"
     CDS             <1..362
                     /locus_tag="RH033_004388"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993477.1"
                     /translation="SVTSKINIKNSAKFNIRSTSRLTYDIISQLHLPQNGYFMERSNF
                     LVAIIMLILRSLAIIPAWFVSLGHEIVTFPFILEIWGNAGDAFSSTSVGNYGFGHYDL
                     NLPQSQLFAGRYGKQTK"
     gene            1163..1879
                     /gene="nanC"
                     /locus_tag="RH033_004389"
     CDS             1163..1879
                     /gene="nanC"
                     /locus_tag="RH033_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HDV2993478.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            1899..3005
                     /gene="nanM"
                     /locus_tag="RH033_004390"
     CDS             1899..3005
                     /gene="nanM"
                     /locus_tag="RH033_004390"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HDV2993479.1"
                     /translation="MNKTITALTIIMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGDNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSADIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            3070..4050
                     /locus_tag="RH033_004391"
     CDS             3070..4050
                     /locus_tag="RH033_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HDV2993480.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDGEDALHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPSIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGSDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVEAILRFWRER"
     gene            complement(4058..4708)
                     /locus_tag="RH033_004392"
     CDS             complement(4058..4708)
                     /locus_tag="RH033_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDV2993481.1"
                     /translation="MNKLPDHLPRRYIIEAIHLWDAGETHQFQPARLYEIEYHGRRYP
                     SKAIAGIAATLMTGTQFTPADFTGGIKSKCVKLLIEQGFHIHQDKAAAAPIANVLFPD
                     ELPSQETYVEGAAIKVTVNRYERDKKARDKAVEYHGCQCNVCGVDLVKIYGDIAEGFI
                     HMHHLVPLSAIKEDYQLDPVNDLLPVCPNCHAMLHRRKPPFSPEELKALMDANKSN"
     gene            4845..5717
                     /locus_tag="RH033_004393"
     CDS             4845..5717
                     /locus_tag="RH033_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDV2993482.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGFWQLHNAELCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTIALPQVRKNYPY
                     EVFVEWHRKEVFRG"
     gene            5762..5941
                     /locus_tag="RH033_004394"
     CDS             5762..5941
                     /locus_tag="RH033_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993483.1"
                     /translation="MQDLAASLQLAFDSGAKRVLLPISSAMDIPTVPAELFTKFQVSF
                     YSEPVDAVYKALDVN"
BASE COUNT         1751 a         1299 c         1365 g         1592 t
ORIGIN      
        1 gttctgtaac aagtaaaatc aacataaaaa attcagcaaa atttaatatt cgttcaacta
       61 gccgtctcac atatgacatt attagtcaac tgcatttgcc acaaaatggc tatttcatgg
      121 aacgcagtaa tttcttagtg gctattatca tgctaattct tagatcccta gcgattattc
      181 ctgcctggtt cgtcagctta ggccatgaga ttgttacatt cccttttatc ctggagatat
      241 gggggaacgc aggcgacgct ttttcatcta catccgttgg taactatggc tttggtcact
      301 acgacttaaa ccttcctcaa tcacagctat tcgccgggcg atacggcaaa caaactaagt
      361 gaaacatcat ccttaaaaca ccaacatcaa caagcctctc cagatcgaca tcaggagtga
      421 ccagttacaa gccacaaacc aagaaacata tttcatgatg agaattatgc tcagcctgat
      481 ggcgggtaaa cacttatttt caccaagctc actgagttag cttgcaaagc tttctttgtt
      541 tagattctaa ataaattatt tataaaacag acacttaaaa gaaaatcttt aatttttgaa
      601 gaccaccgca agtgttcgtc tggcttcaca tggcatctgc ctcttagaaa aagatcgaca
      661 tattttgtga cacgaattgc aaatctggtt ttgttgtatg gattgcgtga tttttgatct
      721 ggtataatag gtataaaggt gcaccaagat agtcaatgag acagggcatc tcgcaatcta
      781 tggcaaacat cacttcagtt ctttctcatc gggtgatgaa aacgcacttc agtctgaaag
      841 gaatatgaaa atgagatcaa cagacactct attttttgac tctgggtaaa atggattgag
      901 taagcgatat agcttacgaa cattcaaatc aattaaacat cagaagagat tttatactca
      961 ggtatttaat ctggctctct gtttatttaa ataatgtgaa aagagatttt tcacaggaga
     1021 ccttatacaa aaaaatataa aatacagcta ccggttgcca aagacactat aagcctggca
     1081 aaaaaaatat tacacaacat aaatgctaat tgtttatgcg ggctttgtat tgctttctgt
     1141 atcctacaaa tgagtgaaat ttatgaaaaa ggctaaaata ctttctggcg tattattact
     1201 gtgcttttcg tccccattaa tttctcaggc tgcgacactg gacgtacgtg gtggatatcg
     1261 tagtggaagc cacgcctatg agactcgact caaagtcagt gagggatggc aaaatggatg
     1321 gtgggcaagc atggaaagta atacctggaa taccattcat gataataaaa aggaaaatgc
     1381 tgcactcaat gatgttcagg ttgaagttaa ttacgcgatt aaacttgatg atcaatggac
     1441 ggtgcgcccg ggaatgttaa cgcattttag cagcaatggc acacgctacg gaccctacgt
     1501 aaaactgtcc tgggacgcga caaaagatct taattttggc attcgctatc gttacgactg
     1561 gaaagcttac cgacaacaag acttatccgg tgatatgtct cgtgataacg ttcatcgttg
     1621 ggatggatat gtcacttacc atattaatag tgatttcacc ttcgcatggc aaacgacgct
     1681 atacagcaaa cagaatgatt atcgctatgc aaaccacaag aaatgggcga cggaaaatgc
     1741 ctttgttcta caataccata tgacgcccga tattacgcca tacatagaat atgactacct
     1801 tgaccgtcag ggtgtttaca acggcagaga taatttatcg gaaaacagtt atcgcattgg
     1861 tgtgtcattt aaactgtagt agacaggaga cagtcacaat gaataaaaca ataacggcgc
     1921 ttactatcat aatggcttca tttgccgcaa acgcgtctgt attaccggaa actcctgtgc
     1981 catttaaaag tggtaccgga gcaattgata acgacactgt ctacattggt ttaggtagcg
     2041 caggtacggc atggtacaag ctggatacac aggccaaaga taaaaaatgg acagcgttag
     2101 ctgcattccc tggcggacca agagatcaag caacctcggc atttattgat ggcaatctgt
     2161 atgtgtttgg cggcattggc aaaaacagcg agggcttgac tcaggtattt aatgacgtac
     2221 acaaatacaa ccccaaaacc aatagctggg ttaaattgat gtcgcacgca ccgatgggca
     2281 tggcaggcca tgtgactttt gtacacaacg gcaaggctta tgttactggc ggtgttaacc
     2341 agaatatctt caatggctat tttgaagatc tcaacgaggc tggaaaagat tcaaccgcta
     2401 tagataaaat caacgctcac tattttgaca aaaaagcaga agattatttc ttcaataagt
     2461 ttctgttgtc ttttgatccc tcaacacagc aatggagtta cgctggcgaa tctccctggt
     2521 acggaacggc tggtgcggcg gttgtgaata aaggtgataa aacctggctt attaatggcg
     2581 aagccaaacc aggattgcga acggatgccg tatttgaact tgacttcacc ggtgataatt
     2641 taaaatggaa taagcttgat cccgtctcat caccagatgg cgtcgctggc ggttttgcgg
     2701 ggataagcaa tgattctctt atatttgccg gaggggccgg attcaaaggt tcacgggaaa
     2761 attaccaaaa cggtaagaac tatgcgcatg aaggcctgaa aaaatcatat agcgctgata
     2821 ttcatctttg gcataacggg aaatgggata aatcgggcga attatcgcaa ggtcgggcct
     2881 acggagtatc attgccctgg aataatagtt tattaattat tggcggtgaa actgcaggcg
     2941 gcaaagcggt gacggattca gttttgatct ctgtgaagga taataaagtc acagtacaaa
     3001 actaatgctt cagggcccca gtaaaggggc cctgctatca atttatcatt tcaattacgc
     3061 gaaaattata tgaacgcaat aatatcgccc gattattact atgttcttac cgttgctggt
     3121 cagtctaatg ccatggcgta tggcgaagga ctgccattac cggacgggga agatgcgctt
     3181 catcccagaa ttaaacaatt agcgagattt gcgcatacgc atcccggagg cccgtcatgt
     3241 cactttaacg acattattcc actgactcac tgcccacacg atgttcagga tatgcagggt
     3301 tatcaccatc ctctggcaac gaatcatcaa acacagtacg gcaccgttgg ccaggcactg
     3361 catattgcac ggaaattact gccctctatt cctgataatg caggggttct catcgttccg
     3421 tgttgccgtg gcggatcggc ttttaccgcg ggcagcgaag ggacatattc agaacggcac
     3481 ggagccagcc atgatgcttg tcgttgggga tcggatactc cgctatacca ggatttagtc
     3541 agcagaacgc gagccgcact ggcaaaaaat ccgcagaaca aattcctcgg cgtatgctgg
     3601 atgcaaggcg aatttgactt aatgaccagt gactacgcgt cacaccctca acactttaat
     3661 catatggttg aagcctttcg tagggatcta aaacaatacc attctcagct taataatatt
     3721 actgacgcac cgtggttttg cggcgatacc acctggtact ggaaagaaaa tttccctcat
     3781 gcgtatgaag cgatttatgg caattatcaa aataatgttt tagccaatat tattttcgtc
     3841 gacttccagc aacaaggtga aagaggactg acgaacgcgc ctgatgaaga tccggacgat
     3901 ttaagcacgg gatattacgg ttcagcgtac cggtcaccgg agaactggac gacggcactg
     3961 cgaagcagtc atttcagcgc ggcagcccgt cgggggatta tttctgacag gtttgtagaa
     4021 gcaattttgc ggttttggcg cgaaaggtga gcgttcatta atttgattta tttgcatcca
     4081 tcaacgcttt taactcctcc ggtgaaaacg gtggtttgcg tcggtgcagc atcgcatggc
     4141 aattagggca cacaggcagc aaatcattaa ctggatccag ctggtaatcc tctttaatgg
     4201 ctgaaagcgg caccaggtga tgcatatgga taaagccttc agcgatatca ccatagatct
     4261 tcaccagatc aacgccgcat acgttgcact ggcatccatg atattcgacc gctttatctc
     4321 gggccttttt atcgcgttca tagcggttta ctgtcacctt tatcgccgca ccttcaacgt
     4381 atgtctcctg cgacggcaac tcatccggga acagaacgtt ggctatggga gccgcagcgg
     4441 ctttatcctg atgaatatga aatccctgct ctatgagtag tttgacgcat ttagatttta
     4501 ttccgccggt gaaatccgca ggggtaaatt gcgtccccgt catcaatgtc gctgcgatgc
     4561 cagcaatcgc cttcgagggg taacgtcggc catgatattc aatctcatac aaccgcgcag
     4621 gctggaactg atgagtttct cccgcatccc ataaatggat agcttcgatg atataccgcc
     4681 gcggaagatg gtcgggaagt ttattcatgg ggaatatcct tatacgttct cagaacaggc
     4741 agcaaaaaaa caaatgagat caaggtaata tatcttcact tgcaggcttg aacagcagaa
     4801 aatttgcgag catgatcgca attatttcac tgccagggat atcaatggct tccagtaaat
     4861 cgctacagca ggcaattgcc aatataaaaa tctggcacaa gggtgaacag cgcgcgccgc
     4921 ataagccatt gttattgcta tacgtattag cgggatacct gaatggacat ccgcgccttt
     4981 tcgattatgg atcggaaatc tacgaacctc tgcacagttt actggaacgt tttgggccac
     5041 agcgttcaca gtaccgacct gatatgccgt tctggcgatt acaaggcgac ggattctggc
     5101 aattacacaa tgccgagctt tgctcaacag cagggagcag ccgacaaccg ccagtgaaag
     5161 aactgaacga ataccacgtt gcaggcggtt tcgatgaaca gcactacgcc ctggtaaccg
     5221 gcaataaaaa actcatcaat actctggcgc aacagatact cgaagcgcac ttcaccgaga
     5281 gtattcagga agaaattgct gacgagctgg gttttgatct ccagcaaatc cgtaaacagc
     5341 gcgacccgtt atttcgcaaa aatgtattac gcgcttataa ctaccagtgc gcgatatgtg
     5401 gctttaatat gcgccacgac gacaccaccg tcgcgctgga agcggcgcat attaaatgga
     5461 aacagcacgg cggtccttgt gaaattccta atggcctggc gctttgcgca attcaccata
     5521 aagcctttga taaaggatcg attggcctgg acgaaaatat gcgggtactg gtatcagatg
     5581 cggtaaacgg cggagggatc gttgagagat tattctggga ttttgacggg aaaactattg
     5641 cgttgccgca ggtgcgtaaa aattaccctt atgaggtgtt tgtagagtgg catcggaaag
     5701 aggtttttag ggggtgaggt tgataactat cgcagtatgt tcaatattgc tgacataatc
     5761 agtgcaggat cttgccgcta gtttacaact cgccttcgat agcggcgcaa aacgagttct
     5821 gttgccgata tcctcggcta tggatattcc aacagttccg gcagagttgt ttaccaagtt
     5881 tcaggtaagt ttttactcag aaccggttga tgcggtttat aaggcgctgg atgtgaatta
     5941 acacattcct cagcttattt acaaaaggat tattcccatt tgatataata tcagtgccaa
     6001 tttactt
//
LOCUS       DAPHCV010000184         5977 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.186, whole genome shotgun sequence.
ACCESSION   DAPHCV010000184 DAPHCV010000000
VERSION     DAPHCV010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5977
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.186"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(47..183)
                     /locus_tag="RH033_004395"
     ncRNA           complement(47..183)
                     /ncRNA_class="other"
                     /locus_tag="RH033_004395"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(228..315)
                     /locus_tag="RH033_004396"
     tRNA            complement(228..315)
                     /locus_tag="RH033_004396"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(279..281),aa:Ser,seq:gga)
     gene            548..1486
                     /gene="ghrA"
                     /locus_tag="RH033_004397"
     CDS             548..1486
                     /gene="ghrA"
                     /locus_tag="RH033_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HDV2993484.1"
                     /translation="MDIIFYHPTFDTQWWIEALHKAIPQTRVRAWKSGDNESADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            1541..2278
                     /gene="ycdX"
                     /locus_tag="RH033_004398"
     CDS             1541..2278
                     /gene="ycdX"
                     /locus_tag="RH033_004398"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HDV2993485.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            2302..2856
                     /gene="ycdY"
                     /locus_tag="RH033_004399"
     CDS             2302..2856
                     /gene="ycdY"
                     /locus_tag="RH033_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HDV2993486.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEGATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            2958..3449
                     /locus_tag="RH033_004400"
     CDS             2958..3449
                     /locus_tag="RH033_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDV2993487.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLIFGYAMKNSGLWLAARSTKTAHREQQIK
                     NKA"
     gene            complement(3513..4346)
                     /gene="csgG"
                     /locus_tag="RH033_004401"
     CDS             complement(3513..4346)
                     /gene="csgG"
                     /locus_tag="RH033_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HDV2993488.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(4373..4789)
                     /gene="csgF"
                     /locus_tag="RH033_004402"
     CDS             complement(4373..4789)
                     /gene="csgF"
                     /locus_tag="RH033_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HDV2993489.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(4814..5203)
                     /gene="csgE"
                     /locus_tag="RH033_004403"
     CDS             complement(4814..5203)
                     /gene="csgE"
                     /locus_tag="RH033_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HDV2993490.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(5208..5858)
                     /gene="csgD"
                     /locus_tag="RH033_004404"
     CDS             complement(5208..5858)
                     /gene="csgD"
                     /locus_tag="RH033_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HDV2993491.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
BASE COUNT         1492 a         1396 c         1481 g         1608 t
ORIGIN      
        1 atacgaaaaa aaagcccgta ctttcgtacg agctcttctt taaagatggc ggtgaggggg
       61 ggattgactc gcttcgctcg ccctgcgggc agcccgctca ctgcgttcac ggtctgtcca
      121 actggctgtc gccagttgtc gaaccccggt cggggcttct catccccccg gtgtgtgcaa
      181 tatacgaaaa aaaagctcgt actttcgtac gagctcttct ttaaagatgg cggtgagggg
      241 gggattcgaa cccccgatac gttgccgtat acacactttc caggcgtgct ccttcagcca
      301 ctcggacacc tcaccaaatt gtcgttcctg tcttgctgga acgggcgcta atttagggaa
      361 atcatgacct gaggtcaaca aactttttta aaaaatcgct cgattattca aacttcaatc
      421 aatatgcagt tttaataagc gaaatttgct ttttttgcca ccgaccacgg atttgttatg
      481 ctggtggtct ttgtagatca taacgataag tgcgaataaa tttcgccaac actcttcggg
      541 agtcagtatg gatatcatct tttatcaccc aacgttcgat acccaatggt ggattgaggc
      601 actgcacaaa gctattcctc agacaagagt cagagcatgg aaaagcggag acaatgaatc
      661 agccgattat gctttagtct ggcatcctcc tgttgaaatg ctggcagggc gcgatcttaa
      721 agcggtgttt gcacttgggg caggtgttga ttctattttg agcaagctac aggctcaccc
      781 tgaaatgctg aagccttctg ttccactttt tcgcctggaa gataccggta tgggcgagca
      841 aatgcaggaa tatgctgtca gtcaggtgct gcattggttt cgacgttttg acgattatcg
      901 catccagcaa aatagttcgc attggcaacc gcttcctgaa tatcatcggg aagattttac
      961 catcggcatt ttgggcgcag gcgtactggg cagtaaagtt gcacagagtc tgcaaacctg
     1021 gcgcttcccg ctgcgttgct ggagtcgaac ccgtaaatcg tggcctggcg tgcaaagttt
     1081 tgccggacgg gaagaactgt ctgcatttct gagccaatgt cgggtattga ttaatttgtt
     1141 accgaataca cctgaaaccg tcggcattat taatcaacaa ttactcgaaa aattaccgga
     1201 tggcgcgtat ctcctcaacc tggcgcgtgg tgttcatgtc gtggaagatg acctgctcgc
     1261 ggcgctggat agcggcaaag ttaaaggcgc aatgctggat gtttttaatc gtgaaccctt
     1321 accgcctgaa agcccgctct ggcaacatcc acgcgtgacg ataacaccac atgtcgccgc
     1381 gattacccgt cccgctgaag ctgtggagta catttctcgc actattgccc agctcgaaaa
     1441 aggggagagg gtctgcgggc aagtcgaccg cgcacgcggc tactaataaa gcatcaggtt
     1501 tcctgctatc cttggcggga attgaataca ggagagagtt atgtatcccg tcgaccttca
     1561 tatgcatacc gttgccagca cgcatgcata tagcacattg agtgattaca ttgcccaggc
     1621 caaacaaaag ggcattaaac tttttgcgat caccgatcat ggcccggata tggaagatgc
     1681 gccgcatcac tggcacttca ttaacatgcg tatctggccg cgagtggttg atggggtagg
     1741 gatcctgcgc ggcatcgaag ctaacattaa aaatgttgat ggtgaaattg actgcagcgg
     1801 aaaaatgttt gattcgctgg atctgattat tgccggtttt catgagccgg tgtttgcgcc
     1861 gcatgacaaa gcgaccaata cgcaagcgat gatcgccact atcgccagcg gcaatgtgca
     1921 tataataagc catcccggaa atccgaaata tgaaatagat gttaaagccg ttgctgaagc
     1981 agccgcgaaa catcaggtgg cgctggaaat caataattcc tcatttttac actcacgtaa
     2041 gggcagtgaa gacaactgtc gggcggtagc cgcagcggta cgtgacgctg gaggttgggt
     2101 ggcattaggc tcggattctc acactgcgtt taccatgggg gaatttgaag agtgtcttaa
     2161 aatcctcgac gcggtagatt ttccgccaga gcgcattttg aatgtttctc cgcgccgctt
     2221 acttaacttc cttgaatctc gcggtatggc accgattgcg gaatttgcag acctttaatt
     2281 actagtaatg gaaatatatt aatgaatgag ttttctatcc tctgtcgtgt gctgggttcg
     2341 ctctattacc gccaaccgca agatccttta ctggtgccgt tgtttaccct gattcgtgag
     2401 gggaaactgg ctgcgaactg gccactggag caggatgagt tactgacgcg tttacagaaa
     2461 agctgtgata tgacgcaagt ctctgccgat tacaatgcgt tgtttatcgg cgatgaatgt
     2521 gctgtgccgc catatcgtag cgcatgggtt gagggcgcga cggaagcgga agtgcgcgct
     2581 tttctttccg aacgggggat gccattagcg gatacgccag ccgatcacat cggcacattg
     2641 ctgctcgcgg cttcctggct ggaagatcag tcaacggaag atgagagcga agcactggaa
     2701 acactgttca gtgagtatct gttaccctgg tgtggtgcgt tccttggcaa agtggaggcc
     2761 catgcaacca cgcctttctg gcgcaccatg gcaccgctaa cccgcgatgc cattagtgca
     2821 atgtgggacg agctggaaga agattctgaa gagtaaatgt gatctacatc actcataact
     2881 gcaacagata atttgttgtt gcataaaatg tgtgctcgat ctcattcatg gccgcgtttt
     2941 ctgctatcat gcgcggcatg aacatacttc tctctattgc aatcacaaca ggcattctct
     3001 ccggtatctg gggatgggtg gctgtttccc tgggcttact aagctgggcg ggcttcctgg
     3061 gctgtacggc ctactttgcc tgcccgcaag gtgggctgaa agggctggcg atctccgctg
     3121 caaccctgct tagtggcgtg gtgtgggcga tggtcattat ttacggtagt gcactggcac
     3181 cacatctgga aattctcggt tatgtcataa ccggtattgt cgcgtttctg atgtgtattc
     3241 aggccaaaca gctgctgctt tcatttgttc cgggaacgtt tataggcgca tgcgcgacgt
     3301 ttgccgggca gggtgactgg aaactggtgt taccttcgct ggcgctcggg ttgatatttg
     3361 gttacgcaat gaaaaacagc ggcttgtggc tggcggcgcg tagtacgaag accgctcacc
     3421 gtgagcagca aatcaaaaat aaagcgtgag gggcactcac gctttcgctt aaacagtaaa
     3481 atgccggatg ataattccgg catttttatc tatcaggatt ccggtggaac cgacatatgg
     3541 cggtatttca ccagaatgtc attctgccgt tctgctttat tttgcaaatc ccaaagaccg
     3601 cggtcgatac catcattaat caggaaaatg acgcctgttt cgatagccga catcaggcac
     3661 agcataacgg gttcgttcga ggtgtaacct acttcccctt ccagcaagcg ctggtagtca
     3721 ataaagcgga agacccccgc ctgaacttca taggaaagta tcgtcttact ggtgttcacc
     3781 gaggagagga tctcgccggt actcacattg acgacgcgca ggttcacggc aatctgatca
     3841 agctggtatt gcgtgtctgc accgatccca aaatatcttg ccccaacccc gcccgattta
     3901 acgttgcttt cataaccgat aattgaacct tcaaccatga tatttgccgc cgttaaagat
     3961 tgcagcggga ttcggttatt aatggcaacc gtgccgtttt cttgtgccgc acgaataatc
     4021 ttgcgctcgt taagcaggtt ttgtaagcct tggcgctcca gcggtataaa ccagcgagaa
     4081 tctttcaatg ccgtgaccag cattgccgtg gcgctttgcg gaacagcagt ggagaagtta
     4141 cttgccgggt agggtttaaa ttgcccggtt tcgtcctgaa tgttgtatac cgaaacaaag
     4201 atcttacccg tcggcgctgg cagatgggtc aaatctttgt agctctgagc acgaggcatt
     4261 aatgtcggtc tggcggcttc tttaggcggg gcggttaagc atccgctcag taacatgacg
     4321 gcaaccaaaa gaaataagcg ctgcatgatt attttcctta tgaagctggg gcttaaaaat
     4381 cggttgagtt attttgtaaa cccgaaacct ggatggtcga ggtttgtccg gttttacgat
     4441 ctgtcacgtt caactgcaat tgaccatcgc ggttagcaat atcaacaata taatcgttgg
     4501 tcaccatgcg gcccggttta ccggtattaa tattcgacag tagcccaccc aaaatttgtg
     4561 actggatggc ctgagtaaag ttatctaacg ccgagggcgt ttcaatacca aagtcatcgt
     4621 tatagctcgg atctttataa gagttttggg cctgagcgct atttaacaaa aaagcgccat
     4681 tatttgggtt accaccaaag tttggattac ggaactggaa agtcatggtt ccagcccaac
     4741 ttaatggcga aataagcatg agtagaacta ctgcatgttt gacacgcatt gcagcctccg
     4801 aacaatttat tatttaaaat tcatcatgcg ccaaatcgcc cgtacttaat aacgcttggt
     4861 ttatctggcg acgatttagt gcttcttcag tttgaatcag tgcaaagacg acagttttct
     4921 cgaagtctct tttcaacgga aataaaaaag tctggaaaat aacgtcctga ttcaccgtta
     4981 ttgtgatcca gcttccccat cgtgcactgg gcctttcatt aatcgttaag ttacccgtat
     5041 agtcactttc ccatttatca ctaaaggctc ggtaaaaatc atggccaata gatgaaacag
     5101 tatggtcagt taacaatccc gggacttcta cctcaacggc gtgaagattc cctgcggcga
     5161 acagaaattc tgccgccaca atccagcgta aataacgttt catggcttta tcgcctgagg
     5221 ttatcgtttg cccaggaaac cgcttgtgtc cggtttttta cggctatctt cttgaaaaga
     5281 ttataaagat gcgttttaac cgtattttcg ctgatgaaca acgaacgagc gatctcgtta
     5341 ttagacgcgc cgatacgcag cttattcagg atctcttttt cccgatgagt aaggagggct
     5401 gattccgtgc tgttataacg atagttacct gaatgcgtaa tcaggtaact ggcaagcttt
     5461 tgcgtaaagt agcattcgcc gcgcaggacg ccttgcaacc cattgacaac acgttcttga
     5521 tcctccatgg cataaaaaac gccgttgata tgaggccagt tttcaatgtc gcggtacggg
     5581 taatcttcag gcgtatttag caacaatatt ttgatattgt tgtttttcct gctcaaagta
     5641 tcctgccaat aatggataag ctttttatcc gcttccatca tatccagaag aataatagag
     5701 cctgaagaga tatcgtccag agaacgttga atattatgta attttcctgt aattgccagc
     5761 gattgcttaa ggtgctgcaa gagagctgtc gcctgcaaag aaggtttagt gatcaacaat
     5821 aatgtatgac catgaatact atggacttca ttaaacatga tgaaaccccg ctttttttat
     5881 tgatcgcaca cctgacagct gcctctaaaa tagaagcacc agaagtactg acagatgttg
     5941 cactgctgtg tgtagtaata aatcagccct aaatggg
//
LOCUS       DAPHCV010000185         5786 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.187, whole genome shotgun sequence.
ACCESSION   DAPHCV010000185 DAPHCV010000000
VERSION     DAPHCV010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.187"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            150..1484
                     /gene="adeQ"
                     /locus_tag="RH033_004405"
                     /gene_synonym="yicO"
     CDS             150..1484
                     /gene="adeQ"
                     /locus_tag="RH033_004405"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HDV2993492.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGVFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISSVIGEVDLSGALSLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1537..1989
                     /locus_tag="RH033_004406"
     CDS             1537..1989
                     /locus_tag="RH033_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HDV2993493.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2200..3390
                     /gene="nepI"
                     /locus_tag="RH033_004407"
     CDS             2200..3390
                     /gene="nepI"
                     /locus_tag="RH033_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HDV2993494.1"
                     /translation="MSEFIAENRGANAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGVALGGFWAISASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAAMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFVGTSLSSFIL
                     KRSVKLALAGAPFVLALSALVLTLWGSDKIVATGVAIIWGLTFALIPVGWSTWITRSL
                     TDQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3431..3724)
                     /locus_tag="RH033_004408"
     CDS             complement(3431..3724)
                     /locus_tag="RH033_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993495.1"
                     /translation="MKPTMLLMITVFLIFPAISQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3946..4764
                     /gene="nlpA"
                     /locus_tag="RH033_004409"
     CDS             3946..4764
                     /gene="nlpA"
                     /locus_tag="RH033_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HDV2993496.1"
                     /translation="MKLTTHHLRAGAALLLAGILLAGCDQSSSDEKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSSEVAKAAETIFNGGAVPGW"
     gene            complement(4768..5691)
                     /locus_tag="RH033_004410"
     CDS             complement(4768..5691)
                     /locus_tag="RH033_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDV2993497.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARDE"
BASE COUNT         1372 a         1310 c         1484 g         1620 t
ORIGIN      
        1 tttgattatt atttatcata aagaaacgtc gcgagattat taaagaatat ccattaatgt
       61 gcaattgaaa tgtgataatt atcactgaac cgttgttgtc atttgtttta caaaagcaag
      121 ggattaaatc tcctccaatg gacaaaaaaa tgaataatga caataccgat tacgtgagta
      181 atgaatcagg gacgctttcg cgattattta aactacctca gcatgggacc accgtccgca
      241 cagaattgat tgcggggatg accacttttt taaccatggt gtacatcgtt tttgtgaacc
      301 cgcaaatcct cggtgcggca caaatggacc cgaaagtggt gtttgttacc acctgtttga
      361 ttgccggtat cggcagtatt gcgatggggg tatttgctaa cttacccgtg gcactggctc
      421 cggcaatggg cctgaacgcc ttctttgcct tcgtggtcgt gggggcgatg ggtatctcct
      481 ggcagaccgg gatgggcgcg atattctggg gcgcagttgg gctatttttg ctcacgctgt
      541 ttcgtatccg gtactggatg atctccaaca ttccattaag tttacgtatt ggtatcacca
      601 gcggaatcgg attatttatc gccttaatgg gattaaaaaa tactggcgtt attgtcgcca
      661 ataaagacac cctggtgatg attggcgatt taagttctca cggcgtgttg ttaggtattt
      721 tagggttttt tattataact gtgttgtcat cacgtcattt tcatgccgcg gtgcttgttt
      781 ctattgtggt gacgtcttgc tgtggattat ttttcggtga tgttcatttt agcggcgtct
      841 attccattcc gcctgatatt agcagcgtca ttggtgaagt ggatttgagc ggcgcgttat
      901 cacttgaact cgccggtatc attttctcct ttatgctgat caacctattt gattcatcag
      961 gaacattaat tggtgtaact gataaagctg gcttaataga tagtaacggt aaattcccta
     1021 atatgaataa ggcgctgtat gttgatagcg tcagttcggt ggcgggtgcg tttatcggca
     1081 cctcgtctgt taccgcctat attgaaagta cttctggtgt ggcagtcggt ggtcgcacgg
     1141 ggctgactgc ggttgtggtc ggcgttatgt tcctgttggt tatgttcttc tcaccgctgg
     1201 tggcgatggt tcctccttac gcaaccgccg gagcgttaat ctttgttggc gtgttgatga
     1261 cctcaagtct ggcgcgcgtt aactgggatg attttaccga atcggtacct gcgtttatta
     1321 ccacggtgat gatgcccttt actttctcga tcaccgaagg gattgcactc ggctttatgt
     1381 cgtactgcat catgaaagtg tgcaccgggc gctggcgcga tctgaacctg tgtgtggtgg
     1441 tggtcgcagc tctgtttgca ctgaagatta ttctggtgga ttagtaactg gcgagggttt
     1501 ccagatatca tgagttctga ttacgcagga gaactcatga tctggataat gctcgccacg
     1561 ctggcggtag tgtttgtggt tggttttcgg gtgctgacat ccggggccag aaaagcgatt
     1621 cgccgtctca gcgatcggct gaacatcgat gtcgtacccg tggagtcgat ggtcgatcaa
     1681 atgggaaagt cagccggtga cgaattttta cgttatttgc atcgtccgga tgagtcgcac
     1741 ctgcaaaacg ccgcgcaggt gttgctcatc tggcaaattg tcattgtcga tggtagcgaa
     1801 cagaacttgc tgcaatggca tcggatttta caaaaagccc gccttgccgc tcccattacc
     1861 gatgctcagg tcaggctggc gctgggtttt ctgcgcgaaa ccgaacctga aatgcaggat
     1921 attaatgctt ttcagatgcg ctataacgcg ttctttcagc ctgccgaggg cgttcactgg
     1981 ctgcattaaa accgcttcgt cttatcagcc tacagaccgc gcctgacctg tagggtggat
     2041 aaggcgttca cgccgcatcc ggtaatcttt gccctgttcc gtttcgatct taactccagc
     2101 tcaagcaaca tcgtaaaagt tgcaaaaacg ttaaatgcat cacacatttc aatgttagac
     2161 tggtcggctt ttcccctgaa acatgccacg ggtaacacca tgagtgaatt tattgctgaa
     2221 aaccgcggcg cgaatgccat cacccgaccg aactggtcag ccgttttctc ggtggcgttt
     2281 tgcgtcgcct gtctgattat cgttgagttt ttgcctgtca gtttgttgac gccaatggcc
     2341 caggatttag gcatttcgga aggggttgcc ggccaatcgg tgaccgtgac cgcctttgtg
     2401 gcgatgtttg ccagtttgtt tattacccag accattcagg ccactgaccg ccgctacgtt
     2461 gtcattctgt tcgccgtttt gctgacgctt tcctgtttgc tggtttcctt tgccaactca
     2521 ttcagtctgc tgttaatcgg tcgcgcctgt ctgggggtgg cgctgggcgg gttctgggcg
     2581 atatcggcgt cgctgaccat gcgtctggta ccgccgcgta cggtgccgaa ggcgctgtcg
     2641 gtgatcttcg gcgcggtttc tattgcgctg gtgattgctg cgccgttggg cagtttttta
     2701 ggcgagctta tcggttggcg caatgtcttt aatgcggcgg cggcgatggg tgtgctgtgt
     2761 attttctgga ttatcaaatc gctgccttcg ctgccaggtg aaccttcgca tcagaaacaa
     2821 aatactttcc gcttattaca acgtccggga gtgatggccg ggatgatcgc catctttatg
     2881 tcctttgccg gacagtttgc ttttttcact tatattcgcc cagtgtatat gaatctggcg
     2941 ggattcggtg ttgatggctt aaccctggtg ctgctgagtt ttggtatcgc cagctttgtc
     3001 gggacatcgc tttcgtcgtt tattctcaaa cgttcggtga aactggcctt agcgggcgca
     3061 ccgtttgtgc tcgccctgag cgcgctggta ctgacgctgt ggggaagcga taaaattgtt
     3121 gctactggtg tggcgattat ctgggggctg acctttgctt taattcccgt tggttggtca
     3181 acgtggatca ctcgctcatt gaccgatcag gcagaaaagg ctggatctat tcaggtggcc
     3241 gttattcagc ttgctaacac ctgcggtgcg gcaatcggcg gttatgcgtt ggataacatt
     3301 ggtctgactt cgccgctgat gttgtccggc acattgatgt tgctgactgc attgttggtg
     3361 actgcaaagg tgaaaatgaa gaaatcctga tatcaggtaa agaagcgttg gtcagtggtt
     3421 gttgccgttc ttacatatcc gggcattcta ccttacccaa cgcttcccaa taattgctct
     3481 gattattacg ttccatcgcc acgatggcct cgcgtatatg ttgtttattg ttttcatctg
     3541 acaacattaa cttttcccac gcttcatctg ataatgacgc ctgtggcgtg caaattttac
     3601 gtaaatcttg caataccgtg gttttctctt ttgccgactg tagagaactg aatggcgact
     3661 cggcctgaga aatggctgga aaaatcagga atacggtaat catgagtagc atcgttggct
     3721 tcatagcggc aaccttctgc aaatgtgcat cgaatgaagc ataagtgtag tttgcttttg
     3781 ccgctctcat catgctgtac gaaaggtgtt tgcgtggtca gtaagaagtg ccagacttta
     3841 tattccactt ttattccttt ttattcttat cgatagcgtt tcgtttttta aactgcagcg
     3901 accttaccgc tatagtcagg taatcattaa taaaaggata aaaaaatgaa actgacaaca
     3961 catcatctac gggcgggggc cgcattatta ctggcaggaa ttctgctggc aggttgcgac
     4021 cagagcagca gcgatgaaaa gcacattaaa gtgggcgtta ttaatggcgc agaacaagat
     4081 gtcgcggaag tcgctaaaaa ggtggcaaaa gagaaatatg gcctcgatgt tgaactggta
     4141 ggatttagcg gttcgttgct accgaacgat gcgacaaatc atggcgagct tgatgcgaac
     4201 gtcttccagc atcgaccctt tcttgaacaa gataatcagg cgcatggtta taaactggta
     4261 gcggtgggaa atacgtttgt cttccccatg gcgggttatt ccaaaaaaat caaaaccgtg
     4321 gcgcaaataa aagaaggcgc gacagtggcg atccccaacg accctaccaa ccttggtcgc
     4381 gcgcttttac tgttgcaaaa agagaagcta attactctga aagagggaaa agggttacta
     4441 cctaccgcgc tggatataac cgataatcct cgtcatttgc agattatgga actcgagggg
     4501 gcgcagctcc cgcgcgtact ggatgatccc aaagttgatg tggcgattat cagcaccact
     4561 tacattcagc agaccggact ttctccggtg cacgacagcg tatttattga agataaaaat
     4621 tcgccgtatg tgaacattct ggtagcacgg gaagataata agaatgcaga aaacgtgaag
     4681 gaatttctgc aatcttatca atcatctgaa gtcgctaaag cggcagaaac catctttaac
     4741 ggtggcgcgg tgcctggctg gtaattctca ctcatcccgc gccggacgat ttatttttct
     4801 ggcgcggcga cgggaatcca ttgaaatcaa aatcactgat gacagaatca gaagcgttcc
     4861 cagccagtcc ggtaatgtga atgtgatccc cagcaacagc aaagagagca gcgcgctact
     4921 taatggttct gcacagctca aaatgctggc ttttggaccg ccaattaatt gtgctccttt
     4981 caggtacaga ctaaatgtca gggacgtacc aatgaccacc aaataaaaaa acgccagaat
     5041 caaactgccg ttaacgacaa agtttgtccc ttgtctggca taaaaaggca acagaatcag
     5101 accgccgatc agcatactcc agccgacgac aggcaacgtg ccatagcggg caatcagcgt
     5161 tgagggatag gtggtataga atgccgcagc aaatgccgag gcaatgcccc agaataacgc
     5221 ggcaggagag atcgataatg acgtcggatt accgtgtgtc accagtaaaa aagtcccgac
     5281 cagcgatgtc aaaatagcgc aaaaaaccag aatgcccggg cgggatttac gcaccagtga
     5341 gaaccaggcg acgataatcg tcggtgagag gaattgcagc accgttgccg tggcagcatt
     5401 cgatttttca atggtcagta aaaaggtgag ctgtacggtt agcgcgccaa ccacggaaaa
     5461 aatcagcaga ctaatggcat ctttgtgatt gttaataata gaaaagattt tatcgccatg
     5521 aacaaatgac agcgtcagca gaatcaaacc ggcgaatatc aaacgcgtca tagtcaaaaa
     5581 ctgcgacgac atctggcttt gctccatgat gtattgcgcg cagacccctg aacttcccca
     5641 caacacggcg gcaatcagaa cgttcagcat cccctttctg gtggaaccca tttttccctc
     5701 tgcatactgt tttgtctttt catcaatata cgttaaggat accactaccg atgacggtga
     5761 gggagtagag taatcaatca gtttta
//
LOCUS       DAPHCV010000186         5656 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.188, whole genome shotgun sequence.
ACCESSION   DAPHCV010000186 DAPHCV010000000
VERSION     DAPHCV010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.188"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(450..1256)
                     /locus_tag="RH033_004411"
     CDS             complement(450..1256)
                     /locus_tag="RH033_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HDV2993498.1"
                     /translation="MSGSVIHSQSAVMVPAVYSAGQPASLPVAIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPDLHRKMLLATLWNTGARINEALALTRGDFSLAPPYPF
                     VQLATLKQRTEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNRR
                     TGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIP
                     LKVLQSLMGHKSISSTEVYTKVFALDVAARHRVQFAMPESDAVAMLKQLS"
     gene            complement(1257..1562)
                     /gene="ccdB"
                     /locus_tag="RH033_004412"
     CDS             complement(1257..1562)
                     /gene="ccdB"
                     /locus_tag="RH033_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HDV2993499.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1564..1782)
                     /gene="ccdA"
                     /locus_tag="RH033_004413"
     CDS             complement(1564..1782)
                     /gene="ccdA"
                     /locus_tag="RH033_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HDV2993500.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERW
                     KAENQEGMAEVARFIEMNGSFADENRDW"
     gene            2350..2862
                     /locus_tag="RH033_004414"
     CDS             2350..2862
                     /locus_tag="RH033_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993501.1"
                     /translation="MTIFKENIVDTSLIGPILTALLAAAGFYCKFWFTRYQASRDQAQ
                     KVSRAIEDVLSKMAALFGQKKPARILREEFSAVIAPLHQEMRILDDMTSRLPLKWLQR
                     EQRHVLYHARWLQRYLDSRRGGSDGDFFLLLHDVAMGTDYQVADILASQHDCKQRRGS
                     QLPVNLKTCP"
     gene            complement(2896..4029)
                     /locus_tag="RH033_004415"
                     /pseudo
     CDS             complement(2896..4029)
                     /locus_tag="RH033_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000545985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
     gene            complement(4196..4969)
                     /locus_tag="RH033_004416"
     CDS             complement(4196..4969)
                     /locus_tag="RH033_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993502.1"
                     /translation="MLILDCSSRTQALHTLSAGFGCSPEKLKKVLLSLDLESIYELNP
                     RQLVDAPQYLREYVCAELGEPGPFTRALWFHGTRTFAGNTFPAGLLALNQSESLAMKM
                     LLDLAPNEMVRTHLKEWDVPGGVPDEMFQLRTGDKIHWGPFGHLVRELHFNASENGLH
                     DYLWLPELVEDVCKAYQKKYGHDLKPHYLSVLHPCIVWFEADIVYEKGVLETALSYAY
                     TSVRDLPPDGNATFGIDCDGKSVSRSAIARIEFLQPGQM"
     gene            complement(4982..5482)
                     /locus_tag="RH033_004417"
     CDS             complement(4982..5482)
                     /locus_tag="RH033_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000528931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993503.1"
                     /translation="MGNYSDFETDFVQRTLALIDQYNEMIEVLGKPFREQYNYTLTLN
                     CLLGLIVLPKERALSFLPADRLTRQLKAEMGLHESQLPGPEMNLRELIHKMRNSVAHF
                     CVQVESASDAHLVDWIVFRESQEDGEVYASFSAPELLPFLKYYATLLLDNMARRRAPD
                     VNILDL"
BASE COUNT         1400 a         1487 c         1373 g         1396 t
ORIGIN      
        1 gcatcgtgtg taagcagaat atataagtgc tgttccctgg tgcttcctcg ctcactcgag
       61 ggcttcgccc tgtcgctcga ctgcggcgag cactactggc tgtaaaagga cagaccacat
      121 catggttctg tgttcattag gttgttctgt ccattgctga cataatccgc tccacttcaa
      181 cgtaacaccg cacgaagatt tctattgttc ctgaaggcat attcaaatcg ttttcgttac
      241 cgcttgcagg catcatgaca gaacactact tcctataaac gctacacagg ctcctgagat
      301 taataatgcg gatctctacg ataatgggag attttcccga ctgtttcgtt cgcttctcag
      361 tggataacag ccagcttctc tgtttaacag acaaaaacag catatccact cagttccaca
      421 tttccatata aaggccaagg catttattct caggataatt gtttcagcat cgcaaccgca
      481 tcagactccg gcatcgcaaa ctgcacccgg tgccgggcag ccacatccag cgcaaaaacc
      541 ttcgtgtaga cttccgttga actgatggac ttatgtccca tcaggctttg cagaactttc
      601 agcggtatac cggcatacag catgtgcatc gcataggaat ggcggaacgt atgtggtgtg
      661 accggaacag agaacgtcac accgtcagca gcagcggcgg caaccgcctc cccaatccag
      721 gtcctgaccg ttctgtccgt cacttcccag atccgcgctt tctctgtcct tcctgtgcga
      781 cggttacgcc gctccatggg tattttcagt gttgccacca tcgtctgcag ctggctgacg
      841 taccaggagt cagagagcgg aaccagccgg tgagtctgct gaccggcggg cattctcccc
      901 gccgtcctgg cagctttttc ggtccgttgt ttcagggtcg caagctgcac aaacggatac
      961 ggaggcgcaa gcgaaaaatc cccccgcgtc agcgccagtg cttcattaat gcgtgctccg
     1021 gtgttccaca gtgtggccag cagcatcttg cggtgcagat ccgggacgta atggagcagg
     1081 gcactcactt ccggagccag cagatatttt ggcagttcat catggaccat cgacatctgg
     1141 cgaagtgcca gagctgccgg ataatcaatg gcaacaggca gcgatgcagg ctgcccggca
     1201 gaatacactg ccggtaccat gactgcagac tggctgtgta taacggagcc tgacatttat
     1261 attccccaga acatcaggtt aatggcgttt ttgatgtcat tttcgcggtg gctgagatca
     1321 gccacttctt ccccgataac ggagaccggc acactggcca tatcggtggt catcatgcgc
     1381 cagctttcat ccccgatatg caccaccggg taaagttcac gggagacttt atctgacagc
     1441 agacgtgcac tggccagggg gatcaccatc cgtcgcccgg gcgtgtcaat aatatcactc
     1501 tgtacatcca caaacagacg ataacggctc tctcttttat aggtgtaaac cttaaactgc
     1561 atttcaccag tccctgttct cgtcagcaaa agagccgttc atttcaataa accgggcgac
     1621 ctcagccatc ccttcctgat tttccgcttt ccagcgttcg gcacgcagac gacgggcttc
     1681 attctgcatg gttgtgctta ccagaccgga gatattgaca tcatatgcct tgagcaactg
     1741 atagctgtcg ctgtcaactg tcactgtaat acgctgcttc atagcacacc tctttttgac
     1801 atacttcggg tatacatatc agtatatatt cttatgccgc aaaaatcagc gcgcaaatac
     1861 gcatactgct atctggcttt tagtaagcct tatgtatttt acctttcgtt atgttaacca
     1921 ataaaaatta aaatctgact tataaaaaca aagcgtaatt accgcattcc cgtttcgtat
     1981 ggatccgact ttatttgaat aaacgacttt ccggaagtgt acaacgctga gaaaatcatc
     2041 atatcgctgt tttccgcccc tgcgcggcgc atcacagtcc agagatacaa cacaacatat
     2101 agtgccatgg gcaactgtat gaggcactac atgctgtgct gacgggatgt aacgctgtgc
     2161 ggagggagag gaagatccgt aacttggggt tttcggatct cagtggagaa aactgaaaga
     2221 aaacaaaaaa ctcacacata aattaaaatg tcaacaccaa aaacaacgaa tagcactttt
     2281 tgcattctcg catagcacta attgctaaaa cggagtatat ttgcgtcatc ggctgcttca
     2341 ggtggcctca tgaccatctt taaggagaat attgtggaca caagtctgat tggaccgatc
     2401 cttacggcgc tgcttgccgc tgcggggttc tactgtaagt tttggtttac gcgttatcag
     2461 gcctcccgtg atcaggccca gaaagtcagc cgggcgatcg aggatgtcct gagcaaaatg
     2521 gccgcgctgt tcggacagaa gaaacctgcc cggatactga gagaggaatt ttccgctgtt
     2581 attgcgccgc ttcatcagga aatgcgtatc cttgacgata tgacgtcccg tctgccgctg
     2641 aaatggctgc agcgcgaaca gcgccacgtt ctttatcatg cccgatggct gcagcggtat
     2701 ctggattcgc gcaggggcgg cagtgacggg gactttttcc tgctattgca cgatgtggcg
     2761 atggggaccg attatcaggt tgctgacatt ctggcatcgc agcatgactg taagcagcgt
     2821 cgtggatcac agctgccggt taacctgaaa acctgcccct aagagggggc agttgaaagc
     2881 agataatatc cggcatcagt cgggtgatcc cgacaccaca tatggcatgc gtctggcttc
     2941 atcaaagtcc cgcagctcct gcgcctgcct catgatctct gcgcttggta ccgggagttt
     3001 acccaggagt tccttaaatc gactggcaac cgactggatt gacacgctgt tggtcctggc
     3061 tgttttcaga ttggtataaa caaggcggga aagtggcttt gtcagcgcct tgtcttccat
     3121 gaatcgcttg aatgtccaga gataaatatc taccaactcc agacctgcac tcttggtacc
     3181 ggactggaat acaagtggtt cagccggcat atttttcatg ttcataacag gtaatccggg
     3241 ccccagttcc cagggcatat cccgtatctg gtagtaaaac tcgttaagtt cacgctgggt
     3301 ggtgttgaac tgtgactgct ggtcaacaat gatactggcc tttctttctg gtgcccccag
     3361 tcgggaacag atcccgtgca atacggactg aaagccaatc atattgggca taatccgcag
     3421 cctttccttg ttggttttac agttatagcc caactgttca aaattggctg acgcccagtt
     3481 cagggcatcg gtgatcagct gcttcgaccg gtggtccagc gcgctggctg ccgtcctgct
     3541 gatcagttca tcactgacgg tacaaaaaag ttccgacgca cgctcatcat gcgcctccag
     3601 ccttgccgtc catgcgtttg aagcaagttc atcatcaaac aggctggcca gattcagcag
     3661 cagggggtag cgcagagggg tccagtatgc agaccagggt acggctggat tcattccctg
     3721 atcaaatacc tggtcgaaga acgaaataat ggcatgatcg cgctttacta cctgccagat
     3781 gtcaaaccgt atccggtgct ttttctgcag aaccagcagc gtatctacaa ggtcagaaag
     3841 gcggtgcaag cccagttccg cagcgtgcag gcgctgcacg ccaagcgttt tgcgtattac
     3901 ggctagatcc gcttcagcaa ccttatccag attagcatca gacgacagca ctccgtagga
     3961 gaggactggc taggttttgt caaaaaggtt tcggccagtc tggcctgttt catccacata
     4021 aaagtgcatt cagagatcct ttaccaggca ggaggaaggg aaacggcata atccgggtca
     4081 atgtaaaacc tttctgcagc cagctgcaag tgatccactt aaggctttaa tgacctcaat
     4141 actgggttca cggcaatgaa caaacgggcc ggatcacaga cccgtttttt agggattaca
     4201 tctgcccggg ctgcagaaac tcgattctgg caatggccga cctagacaca gatttcccgt
     4261 cacagtctat gccgaaagtt gcgtttccgt ccggcgggag gtcacggact gatgtatagg
     4321 catatgacag agccgtttca agaactcctt tttcataaac aatatccgcc tcgaaccaga
     4381 caatacaggg atgaagcacg ctcagataat gcggtttaag atcgtggccg tatttcttct
     4441 ggtaggcctt acagacatcc tctaccagct ccggcagcca gaggtaatca tgcagtccat
     4501 tttcactggc gttaaaatgt aattcccgga ctaaatggcc aaaaggcccc cagtggattt
     4561 tatcgccggt tctgagctga aacatctcat ccggaacgcc ccccggaaca tcccattcct
     4621 tcaggtgtgt acggaccatc tcattcgggg ccaggtcaag cagcattttc atggcgaggg
     4681 attctgactg atttagcgcc agtaaccccg ccggaaaagt attaccggcg aaagtccggg
     4741 tgccatgaaa ccacagagcc cgggtgaatg gtcccggttc gcccagttcc gcacagacgt
     4801 attctctcag atactgaggt gcatcaacca actggcgagg attaagctcg tagatacttt
     4861 caagatcgag cgaaagcagc acttttttca gcttttcagg ggaacagcca aacccggcag
     4921 ataatgtatg caacgcctga gttcgtgagg agcaatcgag aatcaacatg gtgtaaatcc
     4981 cttacaaatc caatatgtta acatcggggg ctcgacggcg ggccatattg tcgagcagca
     5041 gggtcgcata atatttcaga aagggtaaaa gctccggagc gctgaagctg gcgtagactt
     5101 ctccatcttc ttgagattcc ctgaaaacaa tccagtctac aaggtgtgca tcgctggctg
     5161 actctacctg gacgcagaaa tgagcaactg aattccgcat cttatggatc aactcccgca
     5221 ggttcatttc cggtccgggc agctgtgact catgtaatcc catttcagct ttaagttgcc
     5281 gcgttaaacg gtcggcaggc aggaaagaga gcgctctctc tttcggtaac acgataagac
     5341 ccaggaggca attcagggtc aacgtgtagt tgtactgctc cctgaatggt ttgcccagca
     5401 cctcaatcat ttcgttatat tgatcgataa gcgccagtgt tcgctgaaca aaatcggtct
     5461 caaaatctga ataatttccc atttaaccct cccgttccgg ttcgagaatc cactatatca
     5521 tccggccagc ctgacggctc aggtacggca tttcctacta tgatattttt cggatcccgg
     5581 tgaaaaaaga gagaaacatg cagataccat gcatatccat atgtttggca tctgcatatg
     5641 acaggcatac gcatat
//
LOCUS       DAPHCV010000187         5639 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.189, whole genome shotgun sequence.
ACCESSION   DAPHCV010000187 DAPHCV010000000
VERSION     DAPHCV010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.189"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            151..630
                     /locus_tag="RH033_004418"
     CDS             151..630
                     /locus_tag="RH033_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDV2993504.1"
                     /translation="MKTVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFSAMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            642..1118
                     /locus_tag="RH033_004419"
     CDS             642..1118
                     /locus_tag="RH033_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HDV2993505.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            1181..1402
                     /locus_tag="RH033_004420"
     CDS             1181..1402
                     /locus_tag="RH033_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HDV2993506.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            1476..1844
                     /gene="cbtA"
                     /locus_tag="RH033_004421"
     CDS             1476..1844
                     /gene="cbtA"
                     /locus_tag="RH033_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HDV2993507.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLSSCGYVYLAVYPTPEMKN"
     gene            1933..2307
                     /gene="cbtA"
                     /locus_tag="RH033_004422"
     CDS             1933..2307
                     /gene="cbtA"
                     /locus_tag="RH033_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HDV2993508.1"
                     /translation="MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            2304..2498
                     /locus_tag="RH033_004423"
     CDS             2304..2498
                     /locus_tag="RH033_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993509.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAENGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            2511..2624
                     /locus_tag="RH033_004424"
     CDS             2511..2624
                     /locus_tag="RH033_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HDV2993510.1"
                     /translation="MQGIVPLTTETALLSCIKQAREDVLTLRHLQLLHQNR"
     gene            complement(2913..3041)
                     /locus_tag="RH033_004425"
                     /pseudo
     CDS             complement(2913..3041)
                     /locus_tag="RH033_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            3113..3295
                     /locus_tag="RH033_004426"
     CDS             3113..3295
                     /locus_tag="RH033_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HDV2993511.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(3396..3725)
                     /locus_tag="RH033_004427"
     CDS             complement(3396..3725)
                     /locus_tag="RH033_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HDV2993512.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(3897..4955)
                     /locus_tag="RH033_004428"
     CDS             complement(3897..4955)
                     /locus_tag="RH033_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDV2993513.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(5154..5627)
                     /gene="sbmC"
                     /locus_tag="RH033_004429"
     CDS             complement(5154..5627)
                     /gene="sbmC"
                     /locus_tag="RH033_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HDV2993514.1"
                     /translation="MNYEIKQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPGYFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
BASE COUNT         1437 a         1472 c         1357 g         1373 t
ORIGIN      
        1 gataccgaca ttaagctcaa tcgcgcattg tgggtgatgg cagaaacgat gctggagagc
       61 ctgcgctgat gccgtttccc tgagtgaggg aatcattcag ctttcgttcc tgaaaaacac
      121 acatttattt gttatttaag gagttatctc atgaaaaccg tttctcagaa tacccccaca
      181 atttattcag ctacaacacc agagaataat ccgcctcagt tggttgccag cctcgtccct
      241 gatgaacagc gcatcagctt ctggccgcag cattttggcc tcattccaca gtgggtgacc
      301 ctggagcccc gtgtcttcgg ctggatggac cgtctgtgcg aagactactg cggtggtatc
      361 tggaatctgt acaccctgaa caacggcggg gcatttatgg cacccgaacc ggatgacgat
      421 gatgacgaaa catgggtact gttcagtgcc atgaacggta accgcgcaga aatgagcccg
      481 gaagccgccg gtattgccgc ctgtctgatg acgtacagtc atcatgcctg tcgtacggag
      541 aattatgcca tgacggtcca ttattaccgg ttgcgggatt acgccctgca gcatccggaa
      601 tgcagcgcca ttatgcgcat cattgactga ggagagaggc catgcaacag ctttcctttc
      661 tgcccggaga gatgacgccc ggcgagcgca gcctcattct gcgggctctg aaaaccctgg
      721 accgccatct tcatgaaccc ggcgtggcct tcacctccac ccgtgcagca cgggaatggc
      781 tgattctgaa catggcggga ctggagcgtg aagaattccg ggtgctgtat ctgaacaacc
      841 agaatcagct gattgccggt gaaaccctct tcaccggcac catcaaccgc acggaagtcc
      901 atccccggga agtgattaaa cgcgccctgt accacaatgc cgctgccgtg gtactggcac
      961 acaatcaccc gtccggtgaa gtcacaccca gtaaggcaga ccggcttatc accgaacgtc
     1021 tggtacaggc actgggcctg gtggatatcc gggtgccgga ccatctgata gtcggtggca
     1081 accaggtttt ctcctttgcc gaacatggtc tgctttaacc cttcacaacc acatcacacc
     1141 tgttttcact tttatcttct gtcttcagag gtatcccatt atgaaaatta tcacccgtgg
     1201 tgaagccatg cgtattcacc aacaacatcc gacatcccgt ctttttccgt tctgtaccgg
     1261 taaataccgc tggcacggca gtgctgaagc gtataccggt cgtgaagtgc aggatattcc
     1321 cggtgtgctg gccgtgtttg ctgaacgccg taaggacagt tttggtccgt atgtccggct
     1381 gatgagcgtc accctgaact gagtgggaat tctgatgagc agaattatcg ccactaccgg
     1441 accattctta gccgattttc tgtaaggatt ttatcgtgtc agacacactc cccgggacaa
     1501 cacttcccga cgacaatcac gaccgcccct ggtgggggct gccctgcacc gtgacgccct
     1561 gtttcggggc acgtctggtg caggagggta accggttgca ttaccttgcc gaccgcgccg
     1621 gtatcagagg cctgttcagc gatgcagatg cgtaccacct ggaccaggcc tttccgctgc
     1681 tgatgaaaca actggaactc atgctcacca gcggtgaact gaatccccgc catcagcata
     1741 ccgtcacgct gtatgcaaaa gggctgacct gcaaagccga taccctcagc agttgtggtt
     1801 acgtttatct ggctgtttat ccgacgcccg aaatgaaaaa ttaactctcc agaatagcct
     1861 tctgctacgg cctggtgttt tcaccacgcc acttttccat ttttatatct gcatatcagg
     1921 aaaatcttca gtatgaaaac attacctgta ttacccgggc aggcggccag ttctcgcccg
     1981 tctcctgttg aaatctggca gatactgctg tcccgactgc tggaccagca ctatggcctc
     2041 acactgaatg acacaccttt tgccgatgaa cgtgtgattg agcagcatat tgaggcaggc
     2101 atttcactgt gtgatgcggt gaactttctc gtggaaaaat acgcgctggt gcgtaccgac
     2161 cagccgggat tcagcgcctg tacccgctct cagttaataa acagtattga tatcctccgg
     2221 gcacgccggg caaccggcct gatgacccgc gacaattaca gaacggtaaa taacattacc
     2281 ctgggtaagt atccggaggc gaaatgatga cactggaagc cgacagcgtt aacgtacagg
     2341 cgctggatat ggggcacatt gtcgttgaca ttgatggtgt taatatcact gaactgatta
     2401 ataaggccgc tgaaaacggt tattcactcc gcgtggtgga tgaccgtgac tctaccgaaa
     2461 caccggcaac ttatgccagc cctcaccagt tgctgtaaga caatgcagtg atacagggta
     2521 tagtgccatt aaccacagaa acagcactgt tgtcctgtat taagcaggca agagaagatg
     2581 tccttaccct gcgccatctg cagcttctgc accagaaccg gtgaatctca ctcaacaggc
     2641 ttcatttctc ctgacgccag cctgaataca gctggcgttt tcatttataa acagaaagga
     2701 aaaccgataa taatggaatg gacgccccga ccatgaagca acggtaggat attactgata
     2761 aaccttccag tggaggtgcg ttatggccgt tttaacgatt agcattgacc tgacaaaaaa
     2821 tgaattccaa atccacggtc tgggtaggaa cagaaaaatc taagctcaga aaacggatta
     2881 aagtaagcgt caacggagca ccgtattgac gcttatttat tggtgagaac tacgttccat
     2941 ggcaggagtt cgtcaacacg gttggagggc cattccggca gtatgctcag aatatggcgc
     3001 agatacgctt ccggatcgat accgttcaga cggcaggtgc ctgcttttct ggcaacaagc
     3061 aggaatctca gcgctgaagc aggaattaga ggtaaaaacg ccctaccggg ccatgaatca
     3121 cccggtcatt ggagtagtaa ccaaagcaga tttagccagc atggaacaaa tatctttggt
     3181 gaaaagttgg ttacgggaag ctggagcgca caacgtgtta gtaaccagtg cagttaataa
     3241 caatggagtt acagaacttt ttgccttgct gcatacagaa gaaggctgtt gttaatttcg
     3301 attggttctc ggatgagaat gatgacggtg atacacagat aagaatcaat gagcaatgag
     3361 ggttgccggg caaccctcat tgaataaaac gggaattact tcgcttcgcc gtttttcatt
     3421 tcgcccatag ctttcagctt tttggagata tcgcggcgtt ctttggagag ctcggcattt
     3481 ttgatgatgt aatcgtcaac gcgatcttca tagtcacctt tcatgctggc gatgatgccc
     3541 tggattgctt caacgctcat ccccggcttg atgtaatcgc tcaggttgtc cagcagcagg
     3601 acgcgcttct ggttgtcacg gatctttttc tcaacgtcct gaatttcacg ttgcagtttg
     3661 ttcttacgac ggaacagacg aacaaattcc agtacgtcct ggaatgaagg cttggtagtt
     3721 tccattttta tacccctgat aatgtgagag tcggattcgt ttaagcaacc gctattcgtt
     3781 agggccaaca ttactcatag ctgccgcaga tgacaatgct tttatccctt ttcactatca
     3841 taccctttat ccttgctgaa tcgaagcagc agcaagatga ttctgaagtt caggaattat
     3901 ttgcgcaagg cccggcaaat cagattcgat agcaactcaa gctgatgtgc cgtctccatg
     3961 ttcagccaca cataaccata gattggtgtt tccacaacct tcaggtcatg gtggttattg
     4021 agcaactgac gcagctcttc cacagcatcg ttcaattttt ccgtattggc aaaaaccggc
     4081 tgcggattac cttcgtacag cgcatgaaca aggctcagca gtatttgctg catcatatgc
     4141 tgggtatcac gaagtttttg cgcgttcaat aacacgaaat ggctggggcg cgtggcccag
     4201 tacgcattga tttgcaactc cagcatacaa accagattgc gattaatggt ctggatacct
     4261 tcatatatcg attttggaat acgagtttct ttgctggcgg gcgcaatcag cccacgcatt
     4321 ttcacggcat cggtcaggag tttttgtaga tggctttcca gacgtgggcg ttcgagtaag
     4381 ttcggtgaga atgcagattg atagacccga ttatactcgg tcagactttt cgccagttga
     4441 atgcgccagt ggatgaacgc ccgttgtggc cagataccgg taaacaacat tgcgagtaaa
     4501 gagccgagga tcacatcgcc gcttcgccat aacgccgtat caatttcacc tgtcggggaa
     4561 cccacaacaa ttgccagcgt caccccaatc aataatcctt gatacggttt cttgcccagt
     4621 gccagccaac cgcaaagaaa catggccgcc gcgcaccaga ctaacatcag cggtaacgaa
     4681 attaactcca gttgcagagc gataagacct aatatcgaac ccaacaccgt accgccaata
     4741 cgttcgaagg ctcgagggac aacgttaccc cagaacgaga tgggccccat aatcaccacc
     4801 atggtgacca gcggccaggt actttccggg atagtaaaca ggcggataat gagaaaagtg
     4861 agcaggaatg ccagcgcgac ccgagtacca tgcacaatgc ggtaatggcg gtatacccgg
     4921 atttcaaacg ggcttaatga cttatcggca cgcacgcaga aactccgtaa tgaagattaa
     4981 aagcgctcat aactcatctg tcggccagaa ggcgcgcctc tgaattatcc gacggacaaa
     5041 ccgggaacag tgtaatttcc cggcaggagc gaaaaaattg aaaggcgcat cttactcttt
     5101 tcgctttcaa aaaaaagagt ggtcatcgcg tcaacacacc gccctgagat gaattagtga
     5161 tgttttggct gcaccgcaac atacatttcg atatcccagt acccatcttc cgcgccattg
     5221 ttcaaataaa cctcgaagca gggctttggt aacatttcat aagcactgtc ctgcaagagg
     5281 ctattaaaga actgatacca gggtttagca aaatcatcac cgactacacg agctaccgcc
     5341 accgcatact gaccacctga aatttctgtc aaaatgacgc cctcactgtt ttcgggaagc
     5401 gtaaagtaac ccggcaccgt cacgacggtg tcgcagcgta atttttcggc gggtgtttca
     5461 tctggattgt cgtaatagac agcaacccac tccttcggca caatattttt gctatctacc
     5521 cacatcatca actgctcaaa gcctttcttt accgtctgtt cccacgggcc aacgagatgg
     5581 aaacctgcaa cggtacgttt ctcttcctgc ttaatctcgt agttcatgac gcctccatt
//
LOCUS       DAPHCV010000188         5631 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.190, whole genome shotgun sequence.
ACCESSION   DAPHCV010000188 DAPHCV010000000
VERSION     DAPHCV010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.190"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..706
                     /locus_tag="RH033_004430"
     CDS             <1..706
                     /locus_tag="RH033_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDV2993515.1"
                     /translation="KKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFS
                     LESVFILVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVA
                     GVLSAAFYVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVF
                     SSGILQYFLAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFI
                     VTVLSVYFLNRKYSEE"
     gene            complement(1909..2100)
                     /locus_tag="RH033_004431"
     CDS             complement(1909..2100)
                     /locus_tag="RH033_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HDV2993516.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENR"
     gene            2165..2488
                     /locus_tag="RH033_004432"
     CDS             2165..2488
                     /locus_tag="RH033_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HDV2993517.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     ARDDS"
     gene            2430..3188
                     /locus_tag="RH033_004433"
                     /pseudo
     CDS             2430..3188
                     /locus_tag="RH033_004433"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3166..3696
                     /locus_tag="RH033_004434"
     CDS             3166..3696
                     /locus_tag="RH033_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HDV2993518.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSN
                     ESLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFAT
                     TGSLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETN
                     WTKDNGKLNAVRLVQE"
     gene            3703..3936
                     /locus_tag="RH033_004435"
     CDS             3703..3936
                     /locus_tag="RH033_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HDV2993519.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDN
                     AMLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
     gene            4417..5469
                     /locus_tag="RH033_004436"
     CDS             4417..5469
                     /locus_tag="RH033_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993520.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLR
                     KWVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARR
                     GEVSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKD
                     GLKIYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALA
                     DIRNMTIQSVGKGGYSHPEGISTTWHSESNLDRFGCPVHTGNSSYYSGFYHTDVLIPG
                     ELTKDERLLHEIENSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGIPVDDASMFFNSF
                     QPRKFENKPLYEKEDD"
     gene            5471..>5631
                     /locus_tag="RH033_004437"
     CDS             5471..>5631
                     /locus_tag="RH033_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDV2993521.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVI
                     LSWIVLFIK"
BASE COUNT         1486 a         1154 c         1514 g         1477 t
ORIGIN      
        1 aaaaaaactc ccattaaact caaaagcgat acttgcttat gcgacaggcg tttttgaacc
       61 attcttagcg tatactttta cactctacgg actttatttt atttcagcag gaagcgcatc
      121 aattattttt tcacttgaat cagtgtttat attggtgttg tctgttctta tttgctcagt
      181 taaaattaat tccccgcaat attttatatt gttccttata ggtgcaattg ttggcagtat
      241 tttggtagcc ttgccagaca tcagtaatgg atcgaataat attgttggat attgtcttgt
      301 tgttgccggt gtgttatcgg cagcatttta tgttgttata tcgtctcgcc ttattaaatc
      361 gtttgagcct atcacattat tgagcggaca gttgacattc tcccttatac tgtcatcgct
      421 ctttattttt atatctaaat catcaattta tcttccgttg gaatccgtga ttattgtttt
      481 ttcatctgga atattacaat attttttagc tttttgtttt tacttgaact ccttgcgttg
      541 gctcccagtt catttggcag ggataatgtt gtactttatt ccgctagttg cattattttt
      601 gtcatggttg tttttggatg aacaattcac attcttacag ggggtaggat ttattgtaac
      661 tgtgctaagt gtatattttc ttaacagaaa atactcagag gagtaacatt ctgttcagga
      721 ttgccccccg gcctccatcg ataccgtgcc aaatgcacgt aaaaaacggg agcgtttctg
      781 gtgtcggaat gtaaccaagg gggtcagagg cgcgctaaaa cgctctcagg ggagtcaaat
      841 agagacgttt gtaacaaaaa gatcaaagga tcctcttgag atcctttttt tctgcgcgta
      901 atctgctgcc tgtaaacgaa aaaaccgcct tgcagggcgg tttgatcgaa ggttaagtca
      961 gcttgcagac tgcttaacca ggtaactgtc ttatgcgaga cgagtcacca aaattgttct
     1021 tctagtgtag ccgcactccg gcagccactt caagtactct ctaaatatct cgcacatgac
     1081 ttttaccaat ggctgctgcc agtggcgctt tgtggtgtct tttccgggtt ggacccaaga
     1141 gaacagttac cggagaaggc gcagcagtcg ggctgaacgg ggggttcctg catacagccc
     1201 agcccggagc gaactgccta agtcgccccg agataccaga agacgtgagc tatgagaaag
     1261 cgccacgtgt cccgtaaggg agaaaggcgg aaaaggtatc cggtaatcga tatggcggga
     1321 acgtgggggc gcaggaggga gcgattcacc ggaaacggtg atgatcttta agccacgtcg
     1381 ggtttcgcca ccgctggcgg atttcatggt tgagccgcaa ggctcccaca gatgcaccga
     1441 aaaagtgtct gttttgtgaa gtccgtcagg ggggcggagc ctatggaaaa agcgcctccg
     1501 gcgcggcctt tcccggggtt tcgttttttg cgcacatgtt ctttcctggt ttatccccgg
     1561 gttctgtgga taacagggtt agcgtttttg agaaaggaga gtccgctcgc cgcagccgaa
     1621 cgaccgagcg tagcgagtca gtgagcgagg aagcggaaga gcgcctgaac tggcattttc
     1681 tccttacgca tctgtgcggc atttcacacc ggacatggcg cacttttcat acaatccgca
     1741 ctgatgccga atggttaagc cagtatacac tccgctatcg ctacgtgact gggtcagggc
     1801 ttcgccccga caccccaaag gtcaacggcc tgacgcgccc tggcgggctt gtctgctccc
     1861 ggcatccgct cacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agcggttttc
     1921 accgtcatcc ccgaagcgtg cgaggcaact gcggtaaagc tcatcggcgt ggtcgtgaag
     1981 cgattcacag atgtctgcct gttcatccgc gtccagttcg ttgagcttct ccagcagcgt
     2041 taatgtctgg cctctgatga agcgggccat gttaagggcg gttttttcct gtttggtcac
     2101 tgatgcctcc gtgtaagggg gggttctgtt catgggggta atgataccga tgaaacgcga
     2161 gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgctgtga
     2221 gggtaaacaa ctggcggtat ggatgcggcg ggtctgcctg ggtgagccgg ttgcccgttc
     2281 cgggaagctg ccgacactgg caccgccgtt actgcgtcag ctggccgcca tcgggaataa
     2341 ccttaaccag acagcgagga aggtgaacag cgggcagtgg tcttccggtg accgggttca
     2401 ggtggtggcc gcactgatgg ccatcgggga tgagctgcgc cggctgcgtc tggcggtcag
     2461 ggaacagggg gcgcgggatg atagttaagt ttcatgccag gggaaaaggt ggtggcagtg
     2521 gtccggttga ttacctgctg ggaagggagc gtaaccggga aggggcaacg gtgcttcagg
     2581 ggaatccgga agaggtccgg gaactcatcg atgccacgcc atttgcgaag aaatacacgt
     2641 caggtgttct gtcgttcgcg gagaaggagc tgccgcctgg agggcgtgaa aaagtgatgg
     2701 cgagctttga gcgtgtactg atgcccggtc tcgagaaaaa tcagtacagc atcctgtggg
     2761 tggagcacca ggacaaggga cggcttgagc tgaattttgt cattccgaac atggagctac
     2821 agaccggaaa acgcctccag ccgtactacg accgggcaga caggccgaga attgatgcct
     2881 ggcagacgct ggtgaatcac cattacggtc tgcatgaccc caacgccccg gagaaccgcc
     2941 ggacgctgac ccttcctgat aacctgccgg aaacgaaaca ggcgcttgct gagggcgtca
     3001 cacgcgggat agacgcgctt taccatgccg gagagataaa aggccgtcag gatgtgattc
     3061 aggcgctcac agaggcgggg cttgaagtgg tcagggtgac gcgaagcagt atcagcattg
     3121 cagacccgaa cggcgggagg aatatcagac tgaaaggagc gttttatgag caatctttta
     3181 cagacgggcg cggagttcga gaaaaagctg aaagagagag ccgaatctac agagaaaatg
     3241 ctgaacgacg agttcaggaa gctcgaagaa tctgtaaaca gggaactgac ctcaaacgaa
     3301 tctttaatca ggaacgctat caacgaccat acaacagccc tgaaagaact gctggagagg
     3361 tatcagaaaa cgaccgtcga tacgatggac gcgcactgga aaaccgtact gaaaatgagc
     3421 gtgaaacgct ggctgtggct gattatcgtc tcagtactga tgtttgccac gaccgggagt
     3481 ctgctgtggt accagggcat gaagataaac gcgaatatga atattctccg cgagcagaaa
     3541 gagagtctgg agaagctgaa cgcgaagaca tggggcgtgc gttatcacga ggacagcaac
     3601 ggacgttttc tggtgttgcc gaaggggatg aaagccgaaa cgaactggac gaaggacaac
     3661 ggcaaactga acgcagtgag actggtgcag gagtgacaga gcatgacgga gctggaaaga
     3721 catttgctga acgcattaga gcaactgcaa caggactata tgcagcggct gaacgaatgg
     3781 gagaacgcct tcgtggaatt gcagaaaatg ttttcgctta cgcaacggga caacgcgatg
     3841 ctgaacgggc gagtcatgca gttgagtcag caggtgcagc acttgagcga gcggacagaa
     3901 cgcttgagcc agttatacag cgagaacagg agataaggga tgaacggctg atgcaggaac
     3961 gcgaacatga gttatctctg gagcgggagc gtcagccgga aatccaggag cggacactgg
     4021 atggcccttc gctgggatgg tgaaaccatg aaaaatgacg tgttcagtgg attaaggcgg
     4081 gctaatgtgg cctgtagcat ctggttgcat aggtattcat gcggttaaaa tttatcaggt
     4141 gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     4201 gcaccgaacg cgttttggcg gtgcgtacaa tgcgacgtta tggtaaatgg agcggatttt
     4261 caggaaggag tgatactaac aactcgaaca ctgtgcaaat aatgattttt ttaaaatatc
     4321 ggtaacgata ataaattatt tcgttttacg ccggtaataa atataatgta gtttcagtat
     4381 ataaaaaaat atatgaggtg tcttgtggga gctttcatga atcttcaaca acgtataaat
     4441 aaactaccac aattatcatc cagtttttca tttggaaaag atattgataa tattcattca
     4501 tttatcttta acgaaacttc taaagataaa attgaagatt tactaagaaa atgggtgtca
     4561 ggcaatcagc cctgtgtatt tggtaaattg gcaagcaaga aaattaaagg gcttgatttc
     4621 catttatcta ttgtaaatag cccccaacta tataatgatg atggtcattt gtttgatttt
     4681 ttgcgtaatg aaagagttcg ttttaaagaa cgcgcaagaa gaggagaggt cagtgcgcat
     4741 ctcatttatt ttattcatcc acaacttgcc tttgctcgtc catcagaaga gttagtggac
     4801 atacaaaaat atatttgttc gctgcatatg ccagagtgct atccaataaa agaggatgtt
     4861 atttatactg aaagcgtacc atttcaggat aaagatgggc taaaaatata taaggctggt
     4921 gttaatgttt tttactcttc tgctcataga acaagaaatc atgatagaag aataccaggg
     4981 ggaatactta tttctgtaaa tgccccgggg cattttatga ggctggcgat agaaaaaggt
     5041 ttctataaag atcaagaaca agcgttagcg gatattcgta acatgactat tcagtctgtt
     5101 ggtaagggtg ggtattcaca tccggaaggg atttctacaa catggcattc agaatctaat
     5161 ttggatcgtt ttggatgccc agtgcacacc ggtaatagtt cttattacag cggtttttat
     5221 catactgatg tattaattcc aggagaatta actaaggatg aaagattgct ccatgaaata
     5281 gaaaatagtg atccaatgat cttcaactgg aatgtattgt tttatgtttc attagaagag
     5341 tttcctatag atgatcctta ttatggagag tttattggta ttcctgtaga tgatgcatct
     5401 atgtttttta actcctttca accaaggaaa tttgaaaaca aaccccttta tgagaaagag
     5461 gatgattaat atgattaaag gaatgctttt tgctttttta gcagcgttct catggggggc
     5521 agctatcgta atgagcaaaa aaggagttga agaactagat gccggagggg tgtttttctg
     5581 gcagatttgt agtgcagtaa tactatcttg gatagttctt tttataaaaa g
//
LOCUS       DAPHCV010000189         5492 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.191, whole genome shotgun sequence.
ACCESSION   DAPHCV010000189 DAPHCV010000000
VERSION     DAPHCV010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.191"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            127..1461
                     /gene="garP"
                     /locus_tag="RH033_004438"
     CDS             127..1461
                     /gene="garP"
                     /locus_tag="RH033_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HDV2993522.1"
                     /translation="MMLDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1477..2247
                     /gene="garL"
                     /locus_tag="RH033_004439"
     CDS             1477..2247
                     /gene="garL"
                     /locus_tag="RH033_004439"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HDV2993523.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            2277..3167
                     /gene="garR"
                     /locus_tag="RH033_004440"
     CDS             2277..3167
                     /gene="garR"
                     /locus_tag="RH033_004440"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDV2993524.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3264..4409
                     /gene="garK"
                     /locus_tag="RH033_004441"
     CDS             3264..4409
                     /gene="garK"
                     /locus_tag="RH033_004441"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HDV2993525.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVDASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTNDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            4442..4818
                     /gene="rnpB"
                     /locus_tag="RH033_004442"
     ncRNA           4442..4818
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="RH033_004442"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(5384..>5492)
                     /locus_tag="RH033_004443"
     CDS             complement(5384..>5492)
                     /locus_tag="RH033_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HDV2993526.1"
                     /translation="YFKISNTGIDHLEQLINVNFFSSDRTSFCECNRFP"
BASE COUNT         1316 a         1281 c         1520 g         1375 t
ORIGIN      
        1 tgattattat tctcgcgctg cttatactaa ttcccgagtt cattgtcgta acggcataaa
       61 tgaaactcat aaataacacc ttccccataa taataaaaat cagcataagt acccgaggta
      121 aataaaatga tgctggacac cgttgacgaa aaaaagaaag gcgtgcatac ccgctattta
      181 atattactga ttatttttat tgttaccgcc gttaactacg ccgatcgtgc aacgctgtct
      241 attgctggta ccgaagtggc aaaagagttg cagttaagtg cggtttcgat gggttacatc
      301 ttctctgctt ttggctgggc ctacttgctg atgcaaattc ccggcggctg gctgcttgat
      361 aagtttggct cgaagaaagt ttacacttac agcctctttt tctggtcgct gttcaccttc
      421 ctgcaaggct ttgtcgatat gtttccgctg gcctgggctg ggatctccat gttctttatg
      481 cgctttatgc tggggttctc ggaagcgcca tcattcccgg cgaacgcccg tattgtcgcc
      541 gcctggttcc cgacgaaaga acgtggtact gcctccgcca tctttaactc ggcgcaatat
      601 ttctcgttgg cgctcttttc gccgctgctt ggctggctga ctttcgcctg gggctgggag
      661 cacgtcttta ccgttatggg ggtgattggt tttgtgctga cggcgctgtg gatcaagttg
      721 attcataatc cgacagatca cccacgtatg tctgcggaag agctgaagtt tatctcggaa
      781 aatggcgcgg tggtcgatat ggaccacaaa aagccgggca gtgcggcagc aagcggaccc
      841 aaactgcatt acatcaagca attgctctct aaccgcatga tgctgggcgt atttttcgga
      901 caatatttta tcaacaccat cacctggttc ttcctcacct ggttcccaat ttatctggtg
      961 caggaaaaag gcatgtcgat tctgaaagtg ggtctggtcg cctcgattcc ggcactgtgt
     1021 ggctttgcgg gcggcgtgct gggaggtgtc ttctcggatt atctgatcaa acgcggtttg
     1081 tccctgaccc tggctcgtaa gctaccaatt gtgctgggaa tgttgctggc ttccaccatc
     1141 atcttatgta actacaccaa caacaccacg ctggtggtca tgctgatggc gctggctttc
     1201 tttggtaaag gatttggtgc gctgggctgg ccagtgattt ctgacaccgc gccgaaagag
     1261 attgttggcc tctgcggcgg cgtctttaac gtctttggca atgttgcctc cattgtcact
     1321 ccactggtga ttggctacct ggtaagtgaa ctgcactcct tcaatgcagc actggttttt
     1381 gtgggatgtt cagcgctgat ggcgatggtc tgctacctct tcgtagtcgg cgacattaaa
     1441 cgtatggaat tgcagaaata agcaaaggta taagcgatga ataacgatgt tttcccgaat
     1501 aaattcaaag ccgcactggc tgcgaaacag gtacaaattg gttgctggtc agctctctct
     1561 aacccgatta gcactgaagt tcttggtttg gctgggtttg actggctggt gctggatggt
     1621 gaacatgcgc caaacgatat ctccacgttt attccgcagt taatggccct gaaaggcagc
     1681 gccagcgcgc cagtagtgcg agtgccgacc aacgagccgg taattattaa gcgtcttctg
     1741 gatatcggtt tctataactt cctgattcct tttgtagaaa caaaagagga agcagagcag
     1801 gcggtggcat caactcgtta cccaccggaa ggcattcgcg gcgtctccgt ttctcaccgc
     1861 gccaatatgt ttggcaccgt ggcggattat ttcgctcagt cgaacaagaa catcactatt
     1921 ctggtccaga tagaaagtca gcagggcgta gataacgtcg atgctattgc cgctaccgaa
     1981 ggtgtagacg gcatcttcgt cggccccagc gatctggctg cggcattagg ccatctcggc
     2041 aatgcctcgc acccggatgt acaaaaagca attcagcaca tttttaaccg tgccagcgcg
     2101 cacggcaaac ccagcggcat cctcgcgccg gtcgaagccg atgcgcgtcg ttatctggaa
     2161 tggggcgcga cgtttgtggc tgtcggcagc gatctcggcg tcttccgctc tgccactcag
     2221 aaactggctg atacctttaa aaaataacca ccaccgcaac aagagagatg attgatatga
     2281 ctatgaaagt tggttttatt ggcctgggga ttatgggtaa accaatgagt aaaaaccttc
     2341 tgaaagcagg ttactcgctg gtggttgctg accgtaaccc agaagctatt gctgacgtga
     2401 ttgctgcagg tgcagaaaca gcctctacgg ctaaagcgat cgctgaacag tgcgacgtca
     2461 tcataaccat gctgccaaac tcccctcatg tgaaagaggt ggcgctgggt gagaatggca
     2521 ttattgaagg cgcgaagcct ggtacggtat tgatcgatat gagttctatc gcaccgctgg
     2581 caagccgtga aatcagcgaa gcgctgaaag cgaaaggcat tgatatgctg gatgctccgg
     2641 tgagcggcgg tgaaccgaaa gctatcgacg gtacgctgtc agtgatggtg ggcggcgaca
     2701 aggctatttt cgacaaatac tatgatttga tgaaagcgat ggcgggttcc gtggtgcata
     2761 ccggggaaat tggtgcaggt aacgttacca aactggcgaa tcaggtcatt gtagcgctga
     2821 atattgccgc gatgtcagaa gcgttaacgc tggcaaccaa agcgggcgtt aacccggatc
     2881 tggtgtatca ggcaattcgc ggtgggctgg cgggcagtac cgtgctggat gccaaagcgc
     2941 cgatggtgat ggaccgcaac ttcaagccag gcttccgtat tgatctgcat attaaggatc
     3001 tggcgaatgc actggatact tcccacggcg tcggcgcgca actgccgctc acagctgcgg
     3061 ttatggagat gatgcaggca ctgcgagcag atggtttagg aacggcggat catagcgctc
     3121 tggcgtgcta ctacgaaaaa ctggcgaaag tcgaagttac tcgttaatga cgtcgtgccc
     3181 ggatggcgta ttgcaatccg ggcctggaat ccaggccgaa taagagaaac gccctccggc
     3241 gtcatgtggt aacaggcata ggtatgaaaa tcgtaatcgc cccagactct tataaagaaa
     3301 gtttatctgc cagcgaggtt gcgcaggcga tagaaaaagg atttcgggaa atttttcctg
     3361 atgcacagta cgtttctgtt ccggttgccg atggtggcga aggaacggtg gaagcgatga
     3421 ttgcagctac ccagggttct gaacgtcacg cctgggttac agggccgctg ggcgagaaag
     3481 tggatgccag ttgggggatc tccggcgatg gcaaaaccgc gtttattgaa atggcggcgg
     3541 ccagtgggct ggagctggta cctgcggaaa aacgcgatcc actcgtgacc acttcacgcg
     3601 gcacaggcga gttaatcctg caggcgctgg agagcggtgc aacaaacatt attatcggca
     3661 ttggcggcag cgctacaaat gatggcggcg caggcatggt acaggcactg ggggcgaaat
     3721 tatgcgacgc caacggcaat gaaattggtt ttggcggcgg tagtcttaat actctgaatg
     3781 atattgatat ttccggcctc gatccgcgct taaaagattg cgtcattcgc gtcgcttgtg
     3841 atgtcaccaa tccgctggtg ggcgataacg gcgcatcgcg catctttggc ccacaaaagg
     3901 gagccagtga agcgatgatt gttgagctgg acaataacct ctctcactat gccgatgtca
     3961 ttaaaaaagc gctgcatgtt gatgtgaaag atgtccccgg cgcaggagct gcgggcggta
     4021 tgggcgcggc gctgatggcg tttcttggtg cagaactgaa aagtggtatt gaaatagtca
     4081 ctacggcgct gaatctggag gaacatattc acgattgtac gctggtgatc accggtgaag
     4141 ggcgtattga cagccagagt attcacggca aggttccgat tggcgtcgca aacgtggcga
     4201 aaaagtacca taaaccggtg attggcattg cgggtagcct gaccaatgat gttggcgttg
     4261 tacatcagca tggcattgat gcggtcttca gcgtattgac cagcattggt acgttggacg
     4321 aagcattccg cggggcttat gacaatatct gccgtgcttc acgtaatatc gccgcgacac
     4381 tggcgattgg aatgcgcaac gcggggtgac aagggcgcgc aaaccctcta tactgcgcgc
     4441 cgaagctgac cagacagtcg ccgcttcgtc gtcgtcctct tcgggggaga cgggcggagg
     4501 ggaggaaagt ccgggctcca tagggcaggg tgccaggtaa cgcctggggg ggaaacccac
     4561 gaccagtgca acagagagca aaccgccgat ggcccgcgta agcgggatca ggtaagggtg
     4621 aaagggtgcg gtaagagcgc accgcgcggc tggtaacagt ccgtggcacg gtaaactcca
     4681 cccggagcaa ggccaaatag gggttcataa ggtacggccc gtactgaacc cgggtaggct
     4741 gcttgagcca gtgagcgatt gctggcctag atgaatgact gtccacgaca gaacccggct
     4801 tatcggtcag tttcacctga tttacgtaaa aacccgcttc ggcgggtttt tgcttttggt
     4861 gcccagggta agatgaatat ctacggtatt aataatagac tttgtaagaa gttattaagt
     4921 ctatattgca tttaaaaggt cgtaattata catatatttc tctcattaat tgtaagttcg
     4981 caggtattgt ccgatcgttg aatcatttgg gcgttataac ctaaattttt accgtccaat
     5041 aatcaatatt tctgttgtga ttgttaacca ctgcgtgcat tggcgggatt gaagggtgaa
     5101 aaatatgtgc aattgggaag cctttcattg atgcctgcct tgtcatcatg acaaacaata
     5161 atagatgctt attttcctcg cagggttctt tcgcactatc aaaaaatgct catttacccg
     5221 gcattacagt caacgaagca acattctatg gcctacgctg atgtaaagca cagtgagaat
     5281 cccccttgtt gtcttcgtct gaccttatcg cggcgaccag caattattcg ttttgtgatc
     5341 ctcaacacaa aatgctattc gtcgcctatg aggggagagg aatttatgga aaacgattgc
     5401 attcacaaaa tgacgtcctg tcagaagaga aaaagttgac gttaatcaat tgttcgagat
     5461 gatcgatacc tgtattcgaa attttaaaat at
//
LOCUS       DAPHCV010000190         5487 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.192, whole genome shotgun sequence.
ACCESSION   DAPHCV010000190 DAPHCV010000000
VERSION     DAPHCV010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.192"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..248)
                     /locus_tag="RH033_004444"
     CDS             complement(<1..248)
                     /locus_tag="RH033_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="catalyzes the formation of (1 R,6
                     R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     in menaquinone biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HDV2993527.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLV"
     gene            complement(245..1915)
                     /gene="menD"
                     /locus_tag="RH033_004445"
     CDS             complement(245..1915)
                     /gene="menD"
                     /locus_tag="RH033_004445"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HDV2993528.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRILQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     EQHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKNERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(2004..3299)
                     /gene="menF"
                     /locus_tag="RH033_004446"
     CDS             complement(2004..3299)
                     /gene="menF"
                     /locus_tag="RH033_004446"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HDV2993529.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQTFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGEIAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTALNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(3378..3683)
                     /gene="elaB"
                     /locus_tag="RH033_004447"
     CDS             complement(3378..3683)
                     /gene="elaB"
                     /locus_tag="RH033_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HDV2993530.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(3738..4199)
                     /locus_tag="RH033_004448"
     CDS             complement(3738..4199)
                     /locus_tag="RH033_004448"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV2993531.1"
                     /translation="MIDWQDLHHSDLSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     EGENRHILGWHNGTLVAYARILKSDDELQPVVIGRVIVSEALRGEKIGQQLMSKALES
                     CTRRWPEKPIYLGAQAHLQNFYGQFGFIPVTDIYEEDGIPHIGMAREVIQA"
     gene            4264..5181
                     /gene="rbn"
                     /locus_tag="RH033_004449"
     CDS             4264..5181
                     /gene="rbn"
                     /locus_tag="RH033_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HDV2993532.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPHGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEIFDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQALKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCNA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPVTELANDFTVFNV"
     gene            5369..>5487
                     /locus_tag="RH033_004450"
     CDS             5369..>5487
                     /locus_tag="RH033_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HDV2993533.1"
                     /translation="MMVTVVSNYCQLSQTQLCQTFAEKFTVTEELLQSLKKHL"
BASE COUNT         1308 a         1506 c         1355 g         1318 t
ORIGIN      
        1 accagccaga agttaaggat gttgtaacta actaaggttt tacgcagtaa gtcggtgaca
       61 tcatcaaatc catcgacgct aatcgccgcc gaaccaccgt gacctgggag atcaacatac
      121 aaccgtgagt agtcggcaaa cgcctcgccc acttcttgcc attcatggca atcgccggaa
      181 aaaccgtgca aaaacaccag ccagggtaaa cctggtttac cgtgttttgc ctgcgcgtgc
      241 aggatcataa atggcttacc tgcgccagca gttgctggag ggtttgcgca ccatcggtgt
      301 cgttaaccac catttcaatt accgtggtgg tcggcgtgcg ccaggcgtcg gcaagtgccg
      361 tttcaagttc ctgccagttt tgcggacgat gatatttcag ctcgaacatc gcggcggcgt
      421 gctcaaaatg gacgttttgc ggcatcagat agaaacgctc acgctcgttt ttcggcgttg
      481 gcaacagcga gaaaatttgc ccgccgttgt tgttcaccac aattaatacc agcggcgcgg
      541 aaacctgacg taataacgcc agcgcgttga gatcgtaaag tgctgagaga tcgcccacaa
      601 tcgccagcgt cggtttgcca ctggcccgct gaacgccagc ggcggtcgag agcagcccgt
      661 cgataccgct ggcaccacgg ttgctgtaca ccgggtaacc tgccggaagt tgcgaaagcg
      721 catcaatcag acgtaccacc aggctgttac caacaaacaa ttgaccctgt tcaggcagat
      781 agtcgctgat gcgatgcgcc agttgcgctt cgccaaacgc atcgcgacgg gcaataaccg
      841 cctgcattgc ctgttccgcc aggcgcggaa tttcaacgca ccagggctgg cgtttttctg
      901 ccggatgctg ctccagccag tcggcaatat tggcaattaa gcgacgtcca cgatggtgtg
      961 ccggatcaag tcgcccttcg atgtcatcaa caatccagta ctcttccggt tcacagcttg
     1021 cctgccattg caggatccgt ttgcccgtca ggctgcttcc cagttgcacc acaatttgtg
     1081 cctgttgcag ttcgctggtc gctttggcat tgcctaacca gagatcggca cacggcagcg
     1141 gctgtccggt ttgcgacagc acgtcgccaa tcagcggcca gccaagagtt tgcgcccaca
     1201 gtgcaacttt tttgccctct tccgcactca tgcgcccggc aaccaccacg ccgcgctttt
     1261 gccgccagaa gaaccagtcg cgctgttttt cactttccag acgaggcgct tcacgcagcc
     1321 acggtttgtc gtcctgccac cagtcgccca gccgctgttg ccagctaagc ccggtgtcgt
     1381 ccatttcgcc atacagcggt tcagcaaacg ggcagttgat atggactccg cccgcatgaa
     1441 gcgtaccgag agcatggtcg atggtagaaa ccagccaacg tgcaggaata tcctgagtcg
     1501 ggcgcggcag tgaaatactg tgcgtggggt gagaggcgaa cattcccggc tgacgaatcg
     1561 cctgattcgc gccgcagtca attagctccg gcgggcgatc ggcggttaac aggatcagtt
     1621 tttctccggt taacccagct tcaatcagtg ccggatagag atttgctacc gccgtgccgg
     1681 aggtcacaat caccgccacc ggctgcttgc tgactttcgc cagccccagc gccaaatgcc
     1741 ccagtccacg ctcatcgaaa tgggtgtggt gaatgaatgc ggaattctcc gccgccgcta
     1801 acgttaacgg tgtagaacgc gagccagggg cgatacagat gtgtctgacg ccgtgacgcg
     1861 ttaatgcttc cagaatgacc gccgcccagc gtcggttaaa tgcgcttact gacatgagtt
     1921 tgtccggtat caatattgcg gctaagtata aggagctaca aaaatagatt attgatatga
     1981 atcggtaatg atgcgactca ttactattcc atttgtaata aagtacgcag ccctgccgct
     2041 ttgttatcga tttcctgcca ctcttgctcg gggtcagaac cacggacaat gcccgcgcca
     2101 gcatatagcc gcacgacatt gccactgatt tttgctgagc gcagggaaac gcagaattcg
     2161 ctttgttgta aagagagata gcccgcagaa ccggcgtacc attctcgggt gaacggttcg
     2221 tgacgggcga taaactgtcg cgccagatcg cgcggtaagc cagcaactgc tgccgttggc
     2281 tgcaactgat gtaaacagat cacatcatcc gctttgttga gtgcagtcca gatacagcgg
     2341 cgaagatgct gcactttacg cagacgcagt acctgcggcg gtaaaacatc caacgtctgg
     2401 gtatcggcct gcaatcgttg gcagatatcc tccaccacca acatgttctc gcgctggttt
     2461 ttatcatccg ccatcagcca ctcacctaac tgctgcgcct gcttatcatc aggatgattt
     2521 gctaccgttc ccgccagcgc ttcagtacgc agcgctttgt cacgccgccg ccataaccgt
     2581 tccggtgaag agccaagaaa agcaatttcg ccatcaaagg ccatgtaaaa atggtagcaa
     2641 ttcagattca gtcgacgact ggcagccatc atcgccgccg cattgaccgg acttgcgaaa
     2701 tgcaggtcag ttgcccgagc aagcaccact ttgtcgagct caccttcggc gatggttttc
     2761 gttgccagtt cgattaattg cgtccagccc gttttgtccg gccagtgttg ttctcgggtg
     2821 gtggttaaat gtaacccagg caagggttta atactcacca gtgtggcgat aaattctttt
     2881 gccttaatcg catcatgctg aagggagctt tcgctgaata gcgtcagccg cagcgtggct
     2941 ttaccgccac agcgtcgcca ttcgaggcgt ggtaaaagta aattgccctg cgacgggtca
     3001 aacgcattta gcccccagat gcgtaagtcg gcgtgttccg ggtgctgacg aagaaacgtt
     3061 tgtgcctggt ccaacgacgt aaaacgggta atcgcgccca ggacggcagc ttcttcatca
     3121 ccattacgtt gttgccagta gaattgcggg tacgtttgct gactggccag ccagctcaag
     3181 gcatcaaaag cgtctttgag agggaaagga atatcaataa cccgaatgcc gggtgtcgcc
     3241 ggaatctctt gcgacaaatg gcgcagtaga ttttccagcg ccgtagtaag tgattgcacg
     3301 cggacctctc cctgttaaaa gcctcacatt atacggggta cgacaaaaaa aatgcagtac
     3361 cccggtgtag ggaggtttta acggcgtgcc agcaacagtc ctagtaccag cccaacggcc
     3421 gcgcccacac caattccttg ccagggtttt tcgtggacgt agtcatcagc acgataaaca
     3481 gcctgcttcg cccgatagta ataactgtct gaagcctggc taacccgttt tttcacatca
     3541 tccagcgctt tttctgcacg cgctttcagc tcaacatatt tctgatcggc gggatcgcca
     3601 gaggagcgga gcacctcttc cagtgtttca ctaagcagcg tcaggtcatc atcaatacgt
     3661 gtatcaccaa actgattaga cattctcgtt ctccatttgt gtaaaacctg tcactaacta
     3721 tagacaaggg ttattgatta cgcctgaatt acctcgcgtg ccatcccaat gtgtgggata
     3781 ccatcttctt cataaatatc ggtcacaggg atgaaaccaa attgcccata aaagttttgt
     3841 aaatgcgcct gcgcccccag ataaatcggc ttctccggcc agcggcgagt acacgattcc
     3901 agcgccttac tcatcaattg ctgaccgatt ttttcaccgc gtaaggcttc gctgacaatc
     3961 acccgaccaa tcacaacggg ttgcagttcg tcgtcacttt tcagaatcct cgcatacgcc
     4021 accagtgtcc cgttatgcca accgagaatg tggcgatttt ctccctccag atcgtcacca
     4081 tcgatatcct ggtaaggaca gttttgttcc actacaaata ccgcacaacg cagttgtaat
     4141 aaggcataaa gctgagaaac ggataaatca gaatgatgca gatcttgcca gtcgatcatg
     4201 gaaggctcct ttgtgctgtt tgctttatac tagccggaat attttcagca gcgtgatgaa
     4261 ctcatggaat taattttttt aggtacttca gccggtgtgc caacccgtac gcgcaatgtc
     4321 acggcaatat tacttaacct gcaacatccg acccagagcg gtctttggtt gtttgactgc
     4381 ggtgaaggca cccagcatca gctactgcat accgccttta accctggaaa actggacaag
     4441 atttttatca gtcaccttca tggcgatcat ctctttggtt tacccggctt gctgtgcagt
     4501 cgttctatgt caggcattat ccaaccctta acgatttatg gtccgcacgg tatccgtgaa
     4561 tttgtggaga ccgcgctgcg gattagcggc tcatggaccg attatccgct ggaaattgtc
     4621 gaaattggcg ctggcgaaat tttcgatgat ggcctgcgca aagtgaccgc ttatccgctg
     4681 gaacacccac tggaatgcta tggctatcgt attgaagaac atgataaacc gggtgcatta
     4741 aatgctcagg cattaaaagc tgctggcgtg ccgcctggcc cactgtttca ggcattaaaa
     4801 gcgggcaaaa caatcacgct ggaagacgga aggcagatta acggcgcaga ttacttagcc
     4861 gctccagtgc caggtaaagc gctcgctatt ttcggcgata ccggcccctg caatgccgca
     4921 cttgacctgg ctaaaggtgt cgatgtcatg gtgcacgaag cgacgctgga tataaccatg
     4981 gaagccaaag ccaatagtcg cggtcatagc tctacacgcc aggctgcgac actggcccgt
     5041 gaggctggag tcggtaagct aatcattacc cacgtcagct cgcgctatga tgacaaaggg
     5101 tgtcagcacc tgttacgtga atgcaggtca attttcccgg tgactgaact agcgaatgat
     5161 ttcaccgtgt ttaacgttta actctgctta ttattaaaaa cagggcgaaa cttgccctgt
     5221 tatcgcaacc cgcgctacat atatccaccc acacgggtat attgagcgat ttttgatatt
     5281 tattcatctc ctcacgcact atattcagag caataagttg cctcttttta tcttcattca
     5341 gcacaacctt tgcaactaaa aggagcctat gatggttaca gttgtcagca attattgtca
     5401 attatctcaa acccaactct gtcagacatt tgcagaaaaa tttactgtga ccgaggaatt
     5461 actgcagtct ttaaaaaaac acttata
//
LOCUS       DAPHCV010000191         5463 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.193, whole genome shotgun sequence.
ACCESSION   DAPHCV010000191 DAPHCV010000000
VERSION     DAPHCV010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.193"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(436..1455)
                     /locus_tag="RH033_004451"
     CDS             complement(436..1455)
                     /locus_tag="RH033_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDV2993534.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASV
                     KALKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREDAYTLDELADYLDLS
                     ASERRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPV
                     PLSLAECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSENQSD
                     KGKKSAAVRWTANDDKRRRALDMYILGASTEDIAVAVGVSSRTIRRWMDNSGEWLTKK
                     QIIKC"
     gene            complement(2256..3380)
                     /locus_tag="RH033_004452"
     CDS             complement(2256..3380)
                     /locus_tag="RH033_004452"
                     /inference="COORDINATES: protein motif:HMM:NF013211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993535.1"
                     /translation="MSGGDGRGPGNTGLGHNGGKPSGNVNGSSGHGGPSSGGGYHYNP
                     GKGPASTVGPDGEVHINITGGMEKDPPGKHHGNGGSGNGHGAGNHNSNSGGNGKTTSI
                     PGWSPDSGKPGRYLPTNKDKLIKGLIQSIDDHSGTYTIHYTGGAVATVTVPNGDVNKM
                     NVHYNIGSGPSSGNKLTRDTIKSFLEFKAENEANAVNDAISKVNDFYKELASKYGDKL
                     EKEAKALADEAKGKKIRNANEAIKAFDRYKDVLNKKFSVSDREAIAKALESLNKDQMA
                     KQLKIFGKAFGVVGEAIQWGGFISGLVKGFRTGDWNEAFISGEKIAVGKVASVMVTVA
                     FSAMAVNPIGILGFAVIMAVTSALITDERLKQLNSFINGI"
     gene            complement(3601..4134)
                     /locus_tag="RH033_004453"
     CDS             complement(3601..4134)
                     /locus_tag="RH033_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993536.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYR
                     DISDGVAKQVRDEVRNEVQNLDVAGLIGERISQDFQRLEESVANMKANADALNRDIAT
                     IHSNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTMLDRNYNATAAVYQKVVI
                     LEDKLAEKPVTGKNRKN"
     gene            complement(4137..5063)
                     /locus_tag="RH033_004454"
     CDS             complement(4137..5063)
                     /locus_tag="RH033_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HDV2993537.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMP
                     EWARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHA
                     WSFAIHNPKASIEGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSG
                     SLTPTQQKEQLRELRKRWEVKHNEHMRKNGFERGFIDCRTLKEQGIERRPEVHLGFEA
                     AGRLDDAQRHDIMQKRSEPDYSRHLEQARTVGHDIMEWLDTPVKQHELVRRLQHDVAR
                     VHGFDMRDRVSRQDRTEPERVKSPSRGFSMGF"
     gene            5240..>5463
                     /locus_tag="RH033_004455"
     CDS             5240..>5463
                     /locus_tag="RH033_004455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993538.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQER
                     KLLHVLATAEKNDLQAQESERKQAVQKRIEG"
BASE COUNT         1396 a         1451 c         1128 g         1488 t
ORIGIN      
        1 aaaaaaacaa cgacgagaac gcaatcaccg actctttctc gcggctgggc tactgatcga
       61 agcaggactg gtagacacaa aaacaggcga gctatgctat aaaaaagaca ggatattaca
      121 ggcacttaag gaaattaagt acgatctgga gacatcccca aacccagacg cgtaagtgcc
      181 tccatccgtg ttccggttcc ctggtgcttc ctcgctcatt cgctcgctac gctcggtcat
      241 gagactgcgg cgagcacgac cacctcaatc atgcggtaag ccgcaccatt gctgggctga
      301 gaggagaaaa ccgccggaaa gcccgctgct caaaaagacg ccgaaaggcg ctgttatctg
      361 atataggctt gttcatgtcc gcgaatttaa catcccatta acaaaaaacg gggggtagtg
      421 ttgcttcttt gttaactaac atttaattat ttgtttcttg gttagccatt ctccagaatt
      481 atccatccag cgtcgaatag tccggctgct aacaccaacc gccactgcaa tatcttccgt
      541 tgacgctccc aagatataca tatcaagcgc cctacgacgt ttgtcgtcgt tagcagtcca
      601 ccgaacagcg gcgctttttt tccctttgtc cgactgattt tctgacgaat tggccttccc
      661 gcccttgcga ccgcgtgctg cctgaatttc tggcgtgtgc gtatcagcca catactgtgc
      721 gaaagtttcc ggcgtgaagt tcctgtgcgt gtatttcgca atgctcttgc caatagcccg
      781 acattcagcc agtgaaagcg gaacgggaag cgatgcattg tacatttcga cacgctggat
      841 caccgcttca agccactgtg agaacgctgg ccagccctga cgaatcgccc tgtaagccca
      901 tttgcgcgtc atttcgaaca ggtggcagtt tcgccccatc ccgtaatgtt tatcgatgct
      961 acgacgctct gaggcgctca aatcgagata atctgccagt tcatcgaggg tataggcgtc
     1021 ctcgcgccac tccatcacct gccattccag gtggaacgga tttttgcaaa tcaggccgct
     1081 gtagttcaca tcagcacaaa gtttctcaca cagcgaacgt tcaaccgctg cggcatattt
     1141 cagcgcctta accgaggcat caggcgcggt tctcacggcg atgttgagtg cgtagagcag
     1201 gtgagcgtgt ccgttcacag gatttttaac ggtgatgttc ggcgcggggg cgttccggtc
     1261 gctccagtca atcgcagccc cctcgcggtc aacgtcaaaa accagccaga actgcgcatg
     1321 aggctggttc tgctggatgt accgcgcaag aagcgcacgc cctttgccgg agatgcgaag
     1381 gccgaaagcg agatcatccg tgtgataggg gcggtggggt aaattttctt cgaagtattg
     1441 aagcgcggcg ctcatgtgtt acgattcctt ccaggttatc gaccgacctc gcccctgtat
     1501 tctcgtgaca atcttggcgg agagacacga atttcagggg cgtttgtttt tcagtcgctt
     1561 acactacccc caacaacaaa acctgtcgag ccttcccgca aaaccgatcc atttaccata
     1621 actcccgttg tacgtacccc ttaaaacgcc ctcagtcgct ttctggcgca aaaaacgcaa
     1681 aaagacaagg aaacaccagg ccaaaccaga aaaatgcgtc agaatcactt tcagggcgtt
     1741 gcataactat gcacgaaagc gattcgcaaa cagtttcgac cgttcaaaag ggcgaacgag
     1801 tcaaaaaagt aacttttgtg ataactactt ctagatttat ggataaagta aggcaatatt
     1861 ataaacattt aactaacgag gattttataa atgaaaaaaa tagatatgga attctattta
     1921 aggaatttta tccttgcctt atccttttac attttatctt ttatcatcgc aagtttgaat
     1981 ctaagcccaa caagtataga attatttata tttatactta tatctctttt tggatcgtcc
     2041 acataccctt tttgtctatt actaataaat aagcaagcac ccttactaac aaaaacaact
     2101 tttaacttcg ttaactgggg ttttataata tgcattatat tatcactacc tccatttgga
     2161 ttgattttta cagtctatta ctttataacc aacaaaaagc gatagaaaaa caaaataaag
     2221 gtcataccat atcaaacggt acgacctttt gtttattaaa tgccgttaat aaagctgttt
     2281 agttgtttca gcctttcatc tgtaattaag gcactagtca ctgccataat tactgcaaac
     2341 cctaaaatac caataggatt tactgccata gcactaaatg ccactgtaac cataacagat
     2401 gctaccttgc cgactgcgat tttttccccg cttataaaag cctcattcca gtcaccagtt
     2461 ctgaatcctt taactaagcc actgataaat cccccccact gaatagcctc acctacgaca
     2521 ccaaaggcct taccaaaaat ttttaactgt ttagccattt gatctttatt aagagactcc
     2581 agtgcttttg caatcgcttc tcggtcggat acgctgaatt tcttattaag cacatccttg
     2641 tacctatcaa aggctttaat cgcctcatta gcattgcgaa tttttttccc tttagcttca
     2701 tcggctagtg ccttggcttc tttctccaac ttatcgccgt attttgatgc taactcttta
     2761 taaaagtcat taactttgct tattgcatca tttacagcat tagcttcatt ttctgccttg
     2821 aattctaaaa agctttttat agtatcacga gtcagtttat tacctgatga cggcccacta
     2881 ccgatattat agtgaacatt cattttattc acatcaccgt ttggtacagt tactgtagct
     2941 actgcaccac cagtataatg gatagtgtac gttccagaat ggtcatctat tgactgaata
     3001 agtcccttaa ttaacttatc tttatttgtc ggtaaatatc tgccaggttt cccagagtca
     3061 gggctccatc caggaataga cgttgttttt ccattgccgc ctgaattgct gttatgattt
     3121 ccagcaccgt gaccattacc tgagccacca ttaccgtgat gttttcctgg aggatctttt
     3181 tccataccac cggtaatgtt gatatgcacc tccccgtcag gacctacagt gcttgcagga
     3241 ccttttcccg gattgtaatg atacccaccg ccagatgatg gtcctccatg tccagatgag
     3301 ccattcacgt ttccactggg tttaccaccg ttatgtccaa gccctgtatt accagggcca
     3361 cgaccatctc caccactcat aaaaattccc tcttttaatc gttaaaacaa ttaattacat
     3421 agttaatact gtacataaaa ccactggttt tatgtacaga tcaaaaagta ctcgccatta
     3481 ttttttctgt caagtcaggg agggatattt tttggcagtt cggaggaatc actcatgaaa
     3541 acggtgaacg gaacgcgaaa tcctttcgct gtccgtgagg cgttggacaa gcgaagcgcg
     3601 tcagtttttc cggttcttac ccgtgacagg tttttccgcc agtttatctt ccagtatcac
     3661 aactttctga tacaccgccg ctgtggcgtt gtagttcctg tcgagcattg ttttcattcc
     3721 ccatccgagc acacagaaca taactagaaa agccactgac accccaccca gccatttcca
     3781 gctataccct ttaagggtgt tgtattcctg cacgatattg ctgtgaattg tcgcaatatc
     3841 cctgttcagg gcatccgcgt ttgccttcat gtttgcgact gactcttcca gccgctgaaa
     3901 atcctggctg atgcgctcac ctatcagccc tgctacgtcc agattctgca cctcatttct
     3961 gacttcatcc ctgacctgtt ttgccacacc gtcagaaata tcccgatatc cgcgaagtgt
     4021 ttcccttgcc acagccagag aatcagcaac agctttctgt tgctccattg cggtatgttg
     4081 caaatgctcc accgataaca ggagcgtgag taatttttct tctgtgctgg ccatgttcag
     4141 aatcccatag aaaatccacg tgacggtgat ttaacccgtt caggttcagt tcggtcctgt
     4201 ctgctcactc tgtctctcat gtcaaagcca tgcacccgcg caacatcatg ttgcagcctt
     4261 ctgaccagtt catgttgctt aaccggagtg tccagccatt ccatgatgtc atgcccgacc
     4321 gttcgcgcct gttcaagatg gcgggaatag tccggttcag accgtttttg catgatgtca
     4381 tgacgctggg catcatcaag ccgtccggca gcttcaaaac ccagatgcac ttcgggacgt
     4441 cgttcaatgc cctgttcttt cagcgtccga cagtcaatga agcccctttc aaagccattt
     4501 tttctcatat gttcgttgtg cttcacctcc cagcgttttc gcaattcccg caactgctct
     4561 ttttgctgag tgggagtcag gcttcctgaa tcctttttcg cacctccccg ctcgggatac
     4621 ctggcgttat agcgccggaa atactgttcc ggtgtacgtt caatgccatc attcacacgc
     4681 tgactcatca tgatatgcgc atgaggctgt tcgcctccct caatgctcgc tttcggattg
     4741 tgaatggcaa aactccatgc atgccgttcg ccagcttcct gacgtacaaa atcctgcacc
     4801 agttcaaggc gctgttccgg tgtcagttca cggggcagcg ctatctccag ttcccgataa
     4861 gtcgaaccgt ttgcacgttc aaactggtca gccgcctgcc agaaatggga gggattatct
     4921 cgcgcccatt ccggcatgtt tccgtgtgcg gaatattcca aatcttgacg gtcgcggtat
     4981 ttctctttgc gctcgatgta atcggcatgg tttgcagcct gtcccttgcc accaaactta
     5041 acgctgaggt gataggtcgc cacggtttca gccctccgca ggagcgcact gtttctcgac
     5101 gaaggagaga aacatataag tgcgcttcct tctaatttta cgaattagca cggaagatac
     5161 cccctgcacc cgccgaccgc aagcgatata ctgcacgacc acaacacacc gcaaaaaaac
     5221 accagggagc accacgaaca tgcgcatcag caacatcgaa tggctgaaga aacggatcgg
     5281 attcatcagg aagctcggag aacagaccgc acgacagaga cagattattg acctgctcga
     5341 caacgaagca ggactcacag aacaggaacg caaactcctc cacgtactgg caacagcgga
     5401 aaaaaacgac ttacaggcac aggagagcga acgaaaacag gcggtccaaa aacgaatcga
     5461 ggg
//
LOCUS       DAPHCV010000192         5402 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.194, whole genome shotgun sequence.
ACCESSION   DAPHCV010000192 DAPHCV010000000
VERSION     DAPHCV010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.194"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..487)
                     /gene="fdoH"
                     /locus_tag="RH033_004456"
     CDS             complement(<1..487)
                     /gene="fdoH"
                     /locus_tag="RH033_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HDV2993539.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TL"
     gene            complement(500..3550)
                     /gene="fdnG"
                     /locus_tag="RH033_004457"
     CDS             complement(500..3550)
                     /gene="fdnG"
                     /locus_tag="RH033_004457"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(2963..2965),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDV2993540.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSIGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLAANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            3744..4577
                     /gene="fdhD"
                     /locus_tag="RH033_004458"
     CDS             3744..4577
                     /gene="fdhD"
                     /locus_tag="RH033_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDV2993541.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRHDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            4606..5292
                     /locus_tag="RH033_004459"
     CDS             4606..5292
                     /locus_tag="RH033_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HDV2993542.1"
                     /translation="MAGLIHFVNFIILFINKTRVVALTPALQPIDGVAVSYIDAAVAL
                     GNTINEMDKYYIQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYLAAIQEINDKRQL
                     TELKNIEEREGKTFHYYYSLAVMISAKQINNLISQDKFDAEAAMKKVSELETLVAQAK
                     EADKSGMNFSFINSAGQYQLEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMAEDSFPE
                     SIMRVQRNGR"
BASE COUNT         1254 a         1350 c         1457 g         1341 t
ORIGIN      
        1 acagcgtaca tttgtagacg cggttgtctt ccgggttgag gcgcggaatg tcgaacggac
       61 agcccgcaat gcaataaccg cagccgatgc actgctcgga ctggaagtcg acgataccgt
      121 tggcatactg aataattgct ccttccgccg ggcacgcttt caggcagcct ggatcggaac
      181 agtgcataca gccgtctttg cggatcagcc attccagttt gtcgttctgc tccacttccg
      241 agaatcgcat caccgtccac gatttggcac ttaaatcatt ggggttgtcg tacaccccaa
      301 tgttattgcc gacggtatcg cgaatgtcgt tccactctga acacgccacc tgacaggctt
      361 tacagccgat acaggtggtg acgtcgatga gtttcgccac ttcttcctgg aagtcccgcg
      421 cctgaggcgc gggggtcaga ccgttagtcg cggaacgacg aatgatatct tgagattgat
      481 aagccatatg tcgtctccgt tacacctttt ccacattcac aaggaaggac ttaaactccg
      541 gcgtctgcgt gttcgcatca cccacgaatg gcgttaacgt attggcaata aagccttttt
      601 tcgcaacgcc ttcatagccc cagtgaatcg gaataccgat ggtatcgata tctttgccgt
      661 tcgctttcag cgtgcgaata cgtttggtca ccaccgcttt ggctttgata tagccacggt
      721 tggaggagac tttcacagta tcgccctggg caatgccaag tttattcgcc agcgactccc
      781 cgatttccac aaactgctct ggttgcaaaa tcgcattcaa cagcgcgtgt ttggtccagt
      841 agtggaagtg ctcggtcaga cgataggtgg ttccgacata cgggaactta tcggctttac
      901 ccaatgcttc ggcgtcgtct ttaaagatac gcgcagccgg gttcgagata acgtttggat
      961 gcagcgggtt agttcccagc ggcgtttcaa acggctcgta gtgttccggg aacggacctt
     1021 ccgccatctt atcgagggca aacagacgtc ccatgccttc ctgctgcatg ataaacggcc
     1081 ctacgccgct gcccggaggc gctgcgctgt agtccggaat atcccagccg gtccacttag
     1141 tgccgtccca tttcagcaac tgacgcttcg gatcccatgg gttaccctgc ggatctgcgg
     1201 aggcgcggtt atacagaatg cggcggttaa gcggccatgc ccatgcccag cccagcgtgt
     1261 taccgaggcc agacggatcg gcgttatcac gacgcgccat ctggttacct tctggcgtcc
     1321 agctaccggc gaaaatccag cagccacagg aggtcgtacc gtcatcgcgc agttgggcga
     1381 acgagctaag ttgttggcct ttcttgacga taaccgcacc ggttgccgga tcggtaatat
     1441 cggccagcgc cttaccgttg ctctccatcg ccacttcttc cgacttcggc tcatgcggaa
     1501 tggcgtagtt ccaggtcata ttcagcacct ggtccgggtt tgcgccaccc tgttcggcat
     1561 acatcttgcg caagcgcagg aagataccgg agaggatctc gccatcagtc agcgcaatcc
     1621 ctggggcgtc cgcacctttc cagtgccact gtaaccagcg accggagtta acgattgaac
     1681 cgttctcttc tgcgaagcaa gtcgatggca gacggaacac ttcggtctgg atcttcgacg
     1741 agtcaacttc gttcagctca ccgtggttct gccagaagtt agaggtttca gtgttcagcg
     1801 ggtcgatggt gacgaggaac ttcagtttcg acagacaacc gatcactttg tttttgttcg
     1861 ggaatgaggc aacagggtta aagccctggc agatatagcc attgaccttg ccttctttca
     1921 tcatctcgaa gtattgcagg acgtcgtagc ctttatccca cttcggcaac cagtcaaagc
     1981 cccagctatt ttccgccgtc gctttatcac caaagaatgc cttcatcatt gagacgaaga
     2041 atttcgggta gttgccccag tagttgacct ggccttccag cagtggtttt ggcgtgttgg
     2101 cagcgaggta agtttgcaga tcggtctgct tctcgcttgg caacgtcatg taacctggca
     2161 gactctgcga cagcagcccc aggtcggtca gcccctgaat attggagtga ccgcgcaggg
     2221 cgttaacgcc gccgccagcc atccccatat tgccgagcag caactggatc atcgccatcg
     2281 tacgaatgtt ttgcgcacca atggagtgtt gcgtccagcc gagcgcgtaa aggaacgagg
     2341 cagttttatc gtgagcactg gtctcagcga tgtattcgca gactttcagg aatgcgtctt
     2401 ttggcgtacc acagatgttt tcaaccacat ctggcgtgta gcgggaaacg tgctgtttca
     2461 gcaagttcca cacgcaacgt ggatgttgca gggtagtatc gcgtttggcg aagccgtttt
     2521 cgtccagttc gtaagtccag gaggatttat cgtacttacg tttttccgcg tcatagccgg
     2581 tgaacaggcc atcttcaaag ccgtaatcct cacgcacgat caggctggcg ttggtatagg
     2641 cttcggtgta ttcgcggttg aatttttcat tgttcagcag gtacagcaat acgcctgaca
     2701 ggaaagcaat gtcagtaccg gaacgaatag gggcatagta gtcagctacc gccgccgtac
     2761 gcgtaaagcg aggatcgatc acaatcagct tcgcgccatt gtgaattttg gcttccatcg
     2821 cccagcggaa cccgaccggg tgagcttcag cggcgttacc gcccatcacc acgacgaggt
     2881 tggcgttctt gatgtcgacc cagtggttgg tcatcgcacc gcgaccaaat gttggagcaa
     2941 gacttgctac cgttggtccg tgtcagacac gcgcctggtt gtcgaccgcg agcataccca
     3001 gcgcgcggga gaatttttgc gttaaatagc cggtttcgtt actcgacgcg gaagcacaca
     3061 gcatcccggt ggagagccag cggttaacgg tcacgccttc ggcgttttgc gcaatgtagt
     3121 tagcatcgcg gtcttctttc atcagtttgg cgatgcgatc aaacgcctct tcccaactga
     3181 tttgctgcca tttatcagaa cctggcgcac ggtattccgg aaacttcaga cggctttcgg
     3241 agtggatgaa atccaccagg ccagcgcctt tcggacaaag tgcaccgcgg ttgaccgggt
     3301 gatccgggtc accttcgata tggaagatag atgctttggc gttttttgct ccgtcaccga
     3361 ggctgtacat caacagccca cagcctacgg aacaataggt gcaggtatta cgggtttcgc
     3421 gggtgcgcag cagtttatac tgccgggttt ccgcgagtgc tacgctgggt gcaaaaccca
     3481 gtgccgctgc cgtggtgcct gccataccgc cagcgcagat cttaaagaac tgccttctgc
     3541 tgacctgcat ggattgctcc ttgtttcgac attgtcacgt cccatttaca ttcgcttgct
     3601 gcgtgtgcag ggagtgggag ttatttttct ttgcggaagg ggccgcaaag gtccagaatt
     3661 ggctcaattt ccctccatcc aggaagggtt tgtaacagaa taccataatg ttggtgtgtg
     3721 tgttcttatc tggttaagag aaagtgaaaa aaacacagca aaaagaaatc gaaaatgtga
     3781 caaatatcac aggtgttcgt caaattgagt tatggcggca tgatgattta caacacccac
     3841 ggttagatga ggtggcggaa gaagttcccg ttgcgctggt ctacaacggt atttcgcatg
     3901 tggtgatgat ggcgtcgccc aaagaccttg agtactttgc gctcggtttt tcgctttcag
     3961 aggggattat cgaaagtccg cgcgatatct tcggcatgga tgtcgttcct tcctgtaatg
     4021 gtcttgaagt acaaattgag ctttccagtc gccgctttat ggggttgaag gagcgtcgcc
     4081 gggcactggc gggacgtacg ggatgtggcg tgtgcggcgt ggagcaactt aatgacatcg
     4141 gaaaaccggt gcagccgcta ccgttcaccc agacgtttga tctcaacaaa ctggatgatg
     4201 cattacgtca tctcaatgat tttcagccag tggggcaact gaccggttgt actcacgccg
     4261 ccgcctggat gttgccatct ggcgaactgg tcggcgggca tgaagacgtg ggtcgccatg
     4321 tggcgctgga caaactgtta ggccgccggt cacaagaagg ggaaagctgg cagcaaggtg
     4381 cggtgctggt ttccagccgt gccagttatg aaatggtgca aaagtcagcg atgtgtggcg
     4441 tagagatttt gtttgcggtg tctgccgcga ccacacttgc tgtagaagtg gctgaacgct
     4501 gtaatttgac gctggtgggc ttttgtaagc cgggtcgggc aacggtttat acccatccgc
     4561 agcgtttgag caattaatta aaataagtcc tgcgatatat attaaattgc aggacttatt
     4621 catttcgtga attttattat tttatttata aacaaaacgc gtgtagtggc gttaacgcct
     4681 gcgttgcaac ctattgatgg cgtggcggtg agttatattg atgctgccgt agcgttgggt
     4741 aataccatta acgaaatgga taaatattac atccaggaaa attataaaga cgatgcgttt
     4801 gcgaaaggga aaacgctgca ccagacattc ttaaaaaatc tggaagcctt tgaacctgta
     4861 gcagaatctt atcttgcggc gattcaggaa attaatgaca agcgccagct tacggaattg
     4921 aaaaatattg aagaaagaga agggaaaaca ttccactact actactctct ggcagtcatg
     4981 atttcagcga aacaaattaa taacctgata tcgcaagata agtttgatgc agaagcggca
     5041 atgaagaaag tgtctgaact ggaaacgctg gtggcgcagg ccaaagaagc ggataaaagc
     5101 ggcatgaatt tctcgtttat taattcggca ggccagtatc aactcgaggc taaaaaatac
     5161 gttcgccgca tcagagataa agtcccgtac tctgactggg ataaagagca acttcaggac
     5221 gcaaactcaa gctggatggc cgaagactca tttcccgaga gcattatgcg agtacaacga
     5281 aatggtcgat gaaatatttc agtcaattgt aatcgcggag cgggtttgtg atgagcgcgg
     5341 atatgtcacg aagtcgtgac aatataaagc agagtgaagg ataaattttg tgaataaagt
     5401 at
//
LOCUS       DAPHCV010000193         5389 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.195, whole genome shotgun sequence.
ACCESSION   DAPHCV010000193 DAPHCV010000000
VERSION     DAPHCV010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.195"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            158..652
                     /locus_tag="RH033_004460"
     CDS             158..652
                     /locus_tag="RH033_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993543.1"
                     /translation="MKTKRSLVVRVTLITATLLLTACAQPEQSSLAGDWLLTPKDKTR
                     GLTGSIAVNIALSRCKTNCRGDNLPDNTRRWQLSGGNEKELTYLHDMSAQEKAGLNPG
                     WQCYTSFFMQVCQGKPGTRPIVNEDYVSESGFIGSMMHVGIIELRRCQSENCQQELKA
                     INTH"
     gene            complement(693..1195)
                     /locus_tag="RH033_004461"
                     /pseudo
     CDS             complement(693..1195)
                     /locus_tag="RH033_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329477.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2625 family protein"
     gene            complement(1435..2754)
                     /gene="potE"
                     /locus_tag="RH033_004462"
     CDS             complement(1435..2754)
                     /gene="potE"
                     /locus_tag="RH033_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HDV2993544.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYANSWNPHHVPFFSAVGSSIAMTLWAFLGLESACANAEVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLTFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQQVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(2751..4949)
                     /gene="speF"
                     /locus_tag="RH033_004463"
     CDS             complement(2751..4949)
                     /gene="speF"
                     /locus_tag="RH033_004463"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HDV2993545.1"
                     /translation="MSKLKIAVSDSCPDCFTTQRECIYINENSNIDVAAIVLSLNDVT
                     RGKLDEIDATGYGIPVFIATENQERVPAEYLPRISGVFENCESRREFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRADLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEFAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLSEVLPSIYKQHEERYTGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            5072..5329
                     /locus_tag="RH033_004464"
     CDS             5072..5329
                     /locus_tag="RH033_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993546.1"
                     /translation="MQKPLSVRWLIIQGLTENEHCGIQAYACNTESQEDLYAEVPLVG
                     RLTGIEEVKVEAIAMSKLHNMSCPPYMGHSAAVIFHLIYLS"
BASE COUNT         1484 a         1314 c         1251 g         1340 t
ORIGIN      
        1 ttatgactgc ctgatgcgct acgcttatca ggcctgggaa gatggtgcaa tatattgaat
       61 ttacgcgatt ttgtaggccg gataaggcgt tcacgccgca tccggcatga acaacgagca
      121 ctttgccaac aaactgaata gctcaaggga attcaaaatg aagaccaaaa ggagtctggt
      181 cgtcagagtg acattaatta ccgccacact actgctgaca gcatgcgccc aacctgaaca
      241 atcatcatta gctggtgact ggttactgac ccctaaagat aaaaccagag gattaacggg
      301 gagcattgcg gtaaatattg cgctttcccg gtgtaagaca aattgccgtg gcgataactt
      361 acctgataat acccgccgct ggcagttgtc aggcgggaac gaaaaagaac ttacttatct
      421 tcacgatatg tcagcacaag aaaaggccgg tttaaatcct ggttggcaat gctacacctc
      481 attttttatg caggtttgcc agggcaagcc cggcactcga ccgatagtta atgaggatta
      541 tgtcagcgaa agcggcttta ttggctcgat gatgcatgtc gggattattg agttgcgacg
      601 ctgccagtca gaaaattgcc agcaagaatt gaaagcgata aatacacatt aaaatagcga
      661 gtccaggaat gaaacccgga cctgaacatg tcttattgcg tgaatgtttc tttcatttga
      721 tactgcattt cccagagttc agtaagcgaa ataacttttc ttgttcttgc ttcttcactt
      781 tgtacccata agaaagggta gaaagtgaag gcttccgttg ccgataattt tattacgtct
      841 tcttgccatt gcggccagcg tacattttca ttgaaggtat ctaaatcacc ggataatgcc
      901 cactgaagaa actcagaata tccaacctcc agactttccc aaatcagcgt atctggcgca
      961 aaataataaa cgcagccagt atcttcccca agatcgccac cattaagaaa attccccctg
     1021 cgacatcatc ggcgatgggt aaagcccgca cgcttgcgaa ttcagggcgt tgtgtccaat
     1081 cacgcatcgc tacttgctcc ttgtatgcta cggtgtcatt tatgatgagg cattgtatgg
     1141 gacatttttt aaatattaag ttccttatat gtgaataaaa aataacttta tccatacaat
     1201 gccacttttt tcaaagcata aaccgatacc caatactggt ttcagtaatg aaatggcggg
     1261 gcgggcgaga tcctgttcca gtttttgtcg caaatgcctc atataattac gcaaatagtt
     1321 gctgtgtccg accgcgtttg gatcctacac ctgccggatg cggcataaat aaagaactct
     1381 ttgtcaacaa acagaaaggg cgaccattca atcgcccttt tttactttgc tttattaacc
     1441 gtgtttattt ttcagttcaa agcgtggtga gaccagacca tacagtgtcc aaccaaggaa
     1501 ggtcacgatg gaaccgtaca gcatggcttc ttccccggat gagtacagcg catagaaact
     1561 gtacatcgca ccaacaaagg caacaaagtt tgctactttc gcttttgatg gtggcacatt
     1621 agcgacctgc tgaataatga ctaatgctgc catcgacaga atatacggaa tgatattggt
     1681 caccacggcc aggttaacca gcacgttgaa ctggctgttc agtgacgggc taatggtcat
     1741 cagtgccaat ccactctgaa taatcacaat ggtcaacatt ccctgaaccg gtgcatccac
     1801 tttggttaca cgggagaaaa ttttagggaa gtagccttca tcagatgaag atttaaacac
     1861 ctgggcaatg gtgaactgcc agccaagcag tgaaccgcag caggacatca ccatcagcgc
     1921 cataatgact ttacccactt ctggcgtgaa catctgtgcg aaagtcaggc caaatggtgc
     1981 cgttgaattt gccagctcca tattaggcac tatccctgca atcacgttgg tggagacgat
     2041 ataaatcacc gccgcaccta aagtaccgcc aagaactgca atcggcacgt tacgttccgg
     2101 gttttcaact acttcggcgt tggcacaggc tgactccaga ccgaggaaag cccacaacgt
     2161 catggcgatg gaagaaccta cagcgctaaa gaacggcaca tggtgcgggt tccatgagtt
     2221 agcgtatagc gtcgggctaa accagaacca gccgataatg cacagaccga cgaccggaat
     2281 aatgaccccc cacacagtaa tgctactgat ttgcccggta atgcgcgcac caccaaagtt
     2341 agccacggta caaatccaca gcactccgat ggtcgcaaga ccaatctgca ctggcgacaa
     2401 actcgcgccg agcagttcgg tgccgtaacc aaccgccgaa atagcaatcg cgacgttagc
     2461 aatcagcagc gagacaccgt aggtgtagtt cgccataaag ttcccggatt taccgaaagc
     2521 atactccgca taaccgccca taccgcctga tttacggctg aacataccgc attttgcgaa
     2581 tgcccatgcc agtgccattg agcctacggc tgtcaccagc caggagataa ttgagattgt
     2641 cccaacttcg gcaagctttg tcggcagcat gatgataccg gagcccatca tgttgaccat
     2701 cgtcagtatg gttaactgaa cgacgcccat cttattcgat ttagcctgac tcataatttt
     2761 tcccctttca acagggtgct ttgcgcatca cgaggcttga tgacatagca ccaaacttgc
     2821 ttacgaccat catgttcttc gatatagaca ccctgcagct ctggtgcaaa acctggcagc
     2881 aggttgatcc cttcttccag agcgctgaag taacgcagaa cagcaccacc ccagatttca
     2941 cccggaacaa cacacagcac acccggagga taaggaagcg caccttcggc agcgatacgg
     3001 ccttctgcat ccggcagacg aaccagctcc acttcaccgc gtaaataggc gtagttggct
     3061 tcttgtggat tcatgcttac gcgtgggaag tgttccttac ggaacatctc tttttgcagt
     3121 tgtttcacgt tgtgacgggc atacaaatca tgcatttcct gacacaactg acgcagggta
     3181 taaccggtgt agcgctcttc atgctgtttg tagatggaag gcagtacttc tgataatggc
     3241 gcgtcggact caagcagttt ttcgaagcgt accagcagag caacaagttg ctgaagtttg
     3301 gccatatctt ctgccggagt cagcagaaag aggatggagt taagatcgca tttttccggc
     3361 actacgccat tttcgcgcag gaagttagca agaatcgtcg cgggtacacc gaacgcttca
     3421 tattcgccgt tacgtgcatc aatacctggg gttgtcagca acagtttgca cggatcgaca
     3481 aagtattgat tctctgcata gccttcaaaa gaatgccagt gctcacctgg tacaaactgg
     3541 aagaagcgca gatcaaccgc aatttgcgcc gtttcatacg actgccaggg tttaccatcc
     3601 accagttcag gtacgaatgg acgaatatgc tgacagttat cgaggatcag tttgcgggca
     3661 ttaatgccat ttaccacaca atccatccac atattacgac cgctgacacc ttcatgcatt
     3721 ttggcgttga tatccagtgc ggcaaacagc ggatagaacg ggctggtgga ggcatgcatc
     3781 ataaaggcgt tgttcatgcg tttgtgcggt acataacgct gttgcccttt gatatggctg
     3841 tcttttttat ggatttgtga agtctgagaa aaaccagcct gctgtttatg cacagattgc
     3901 gtaaccagaa tacccggatc gttctcatta agatccagca acagtggtga acagtccgcc
     3961 atcatcggga taaactgctc atagccgacc catgcggagt caaagaggat gtagtcacac
     4021 agatgaccaa ttttatccac cacctgacgg gcgttataaa tcgtaccgtc gtaagtgcct
     4081 agctgaataa cggcgaggcg gaaaggacga gcctcttttg cccgctgcgg tgcgaattcc
     4141 gcgatcagtt cacgcaggta actttcttca aaacagtgag cgtcaatacc accgataaaa
     4201 ccatacgggt tacgtgcggt ttccagataa accggtgtcg cgccagcctg tagcaacgct
     4261 ccgtggtggt tagatttgtg gttattgcgg tcaaacagca ccagatcacc cggtgtcagc
     4321 agggcgttta aaaccacttt gttagaagat gaagtgccat ttaaaacgaa gtatgtttta
     4381 tcggcattaa acacttttgc cgcatgttgc tgtgcaatgc atggcgcgcc ttcgtgaatc
     4441 agcagatcgc ccatcgctac gtcggcgttg cacaagtcag cacggaacag cgcctcacca
     4501 aagtattcca caaactgatt ccccgcggga tggcgacgga aaaattcgcc gccctgatga
     4561 ccggggcaat caaacgcgct gttaccctga ttgacataat cgaccagtgc gcggaagaag
     4621 ggcgggcgca gttgcgtttc ataatggctg gctgcggttt ctaactgacg accataaaat
     4681 tctcgtcgcg attcgcaatt ctcaaagaca cccgaaatac ggggcaaata ctctgcgggt
     4741 acacgttctt gattttcagt agcaataaat accgggatgc cataacccgt ggcatcgatt
     4801 tcatcgagtt ttccgcgtgt aacatcgttg agcgataaaa ctattgccgc cacatcgata
     4861 ttactgtttt cattaatata gatacattct cgctgcgtgg taaaacagtc cgggcaagaa
     4921 tcactaaccg caatttttaa ttttgacatt ttccatctct ttattttggg taatagcagg
     4981 tcctcaattt ctcgaatgag aaatcgatcg tgccggaaaa aaagcaaagt taccccgctg
     5041 ataaattaat cagaagtttg cttttttcag aatgcagaaa cccttatcag tccggtggct
     5101 gataatacaa gggttaacag agaatgagca ctgcggaata caggcatatg cctgtaatac
     5161 agagagtcag gaagacctgt acgctgaagt gcccttagtc ggtagactaa cgggcattga
     5221 agaagtgaaa gtcgaagcta ttgcgatgag caaacttcat aatatgagtt gtccgcctta
     5281 tatggggcat agtgcggctg ttattttcca tttaatttac ctttcctgat gtgaatgaag
     5341 gtgaaggtat tctatccagg aaatacggtt gaactgtgaa ggtgatcaa
//
LOCUS       DAPHCV010000194         5366 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.196, whole genome shotgun sequence.
ACCESSION   DAPHCV010000194 DAPHCV010000000
VERSION     DAPHCV010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.196"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            114..1457
                     /locus_tag="RH033_004465"
     CDS             114..1457
                     /locus_tag="RH033_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDV2993547.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQRGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGETHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQARSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            1682..3337
                     /gene="opgD"
                     /locus_tag="RH033_004466"
                     /gene_synonym="mdoD"
     CDS             1682..3337
                     /gene="opgD"
                     /locus_tag="RH033_004466"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HDV2993548.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTDFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            3477..3701
                     /locus_tag="RH033_004467"
     CDS             3477..3701
                     /locus_tag="RH033_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HDV2993549.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            3764..4303
                     /gene="rimL"
                     /locus_tag="RH033_004468"
     CDS             3764..4303
                     /gene="rimL"
                     /locus_tag="RH033_004468"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HDV2993550.1"
                     /translation="MTETIKVSESLELHAVAENHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SYQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNAQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDSQ"
     gene            complement(4295..5275)
                     /locus_tag="RH033_004469"
     CDS             complement(4295..5275)
                     /locus_tag="RH033_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HDV2993551.1"
                     /translation="MRKYRLSEEQRAFSYQDDGTKKSVLLRQIIAMSDFNDVIAETAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIEKHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
BASE COUNT         1370 a         1291 c         1332 g         1373 t
ORIGIN      
        1 cgaaatggat aatattgatg ttttcgcggc gagatcacag tttgcaaatt cttcccgcca
       61 gagtgaatgc ggttacctac actccagatt actgaccact ggaggcagac attatggcga
      121 acagcatcac ggcggatgag attcgggaac agttttcgca ggcaatgtca gccatgtacc
      181 agcaagaagt tccgcagtac ggtacgctgc tggaactggt agctgatgtg aatctggctg
      241 tgctggaaaa caatcctcaa ctgcacgaaa aaatggtaaa tgcagacgag ctggcgcgac
      301 tgaatgttga acgtcatggg gcgattcgtg ttgggactgc acaagagctt gctactcttc
      361 ggcggatgtt tgccattatg gggatgtacc cggtgagcta ttacgatctc tcgcaggcgg
      421 gggtgccggt acattcgaca gcatttcggc ccattgatga tgcttctctg gcgcgtaatc
      481 ccttccgcgt ttttacttca ttactccgcc ttgagcttat cgagaacgaa attttgcgcc
      541 agaaagcggc ggagattctg cgtcagcgcg atatcttcac cccacgttgt cgacaactgt
      601 tagaggaata tgagcagcgg ggcggtttta acgaaacaca ggcacaggag tttgtgcagg
      661 aagccctgga aacgtttcgt tggcaccagt cagcaacggt agatgaagaa acctatcgcg
      721 ccttgcacaa cgaacatcgg ttgattgctg atgtggtctg ttttcctgga tgccatatca
      781 accacctgac gccacgcacg ctggatattg accgggtgca gtcgatgatg cctgaatgcg
      841 gaattgaacc caaaattctg atcgaagggc cgccgcgccg cgaggtaccg attttactac
      901 gccagactag ctttaaagca ctggaagaga cggtgttgtt tgcagggcag aaacagggca
      961 cgcataccgc gcgctttggt gaaatagagc aacgtggcgt ggcattaacg ccgaaaggac
     1021 gacaactgta tgatgatcta ctgcggaacg ctggaaccgg gcaggataat ctcactcacc
     1081 aaatgcattt acaggaaacc ttccgcactt ttcctgacag tgagttttta atgcgtcagc
     1141 aagggttggc atggttccgg taccgtctga cgccttcagg tgagacgcat cgtcaggcga
     1201 ttcatcccgg agacgatcca cagcccttaa ttgaacgtgg ttgggtagtg gcgcaaccca
     1261 ttacctatga agatttcttg cctgttagcg cggcggggat cttccagtca aatctgggta
     1321 atgaaacgca ggcacgcagt cacggtaatg ccagtcgcga agcatttgag caggcgttgg
     1381 gctgcccggt tttggatgag ttccagcttt atcaggaagc ggaagaacgc agtaaacgtc
     1441 gctgtggttt gctttaaatt ctgaccatcc gcctttgcaa aaatttgcct gattttacaa
     1501 acgaatcagg ctcatcccat cgacataaaa aaattgccga tttatgcata ttctctcagt
     1561 tcaacaattg gattattaat aaatattgtc tagagtgagc ggtcataaat aagcactttc
     1621 ttgccgctga aaacgaccag cgcgggacca ttcacaacac cagaaggact cactttcagg
     1681 tatggatcgt agacgattta ttaaaggttc aatggctatg gccgccgtgt gcggtaccag
     1741 cggcattgct tctctttttt ctcaggcggc attcgcggca gattctgata ttgccgacgg
     1801 gcaaacccaa cgttttgact tctccattct acagtcaatg gcgcacgact tagcgcaaac
     1861 agcgtggcgt ggtgcgccgc gtccgttacc tgacactctg gcgacaatga cgccgcaggc
     1921 ttataacagt attcaatacg acgccgaaaa atcgctctgg cataacgttg agaaccgtca
     1981 actggacgct cagttcttcc atatgggaat gggattccgt cgccgcgttc gtatgttttc
     2041 tgttgatccc gcaacacatc tggcgcgtga aattcacttt cgcccggagt tgttcaaata
     2101 caacgatgcg ggtgttgata ccaaacaatt agaagggcaa agcgatctcg gttttgccgg
     2161 ttttcgcgtg tttaaagccc ccgaactggc gcgccgtgat gtcgtatcat tcctcggcgc
     2221 gagttatttc cgcgcggttg atgatacata tcaatacggt ttgtcggccc gcggcctggc
     2281 gatcgacact tacaccgaca gtaaagaaga gttccccgac tttactgact tctggtttga
     2341 tacggtaaaa ccgggggcaa ccacctttac cgtttatgcg ttgctcgata gcgccagcat
     2401 tactggtgcc tataagttca ctatccattg tgagaaaagt caggtgatta tggatgtgga
     2461 aaatcacctg tatgcgcgca aagacattaa acagctgggc attgcgccga tgaccagcat
     2521 gttcagctgc ggtactaatg aacgtcggat gtgcgacacc attcatccgc aaatccatga
     2581 ctcagatcgt ctgtccatgt ggcggggcaa tggcgagtgg atttgtcgtc cgctgaataa
     2641 tccgcaaaaa ttgcagttca atgcttacac cgacaacaac ccgaaagggt ttggtttatt
     2701 gcaactggat cgtgatttct cccattatca ggacattatg ggctggtata acaaacgccc
     2761 aagtctgtgg gtggagccgc gtaacaagtg gggtaagggc accatcggcc tgatggaaat
     2821 cccaacaacg ggcgaaacgc tggataacat tgtctgcttc tggcagccag aaaaagctgt
     2881 gaaagcgggt gatgagtttg cattccagta tcgtctgtac tggagtgcgc aaccgcctgt
     2941 tcattgccca ttagcgcgcg ttatggcgac gcgtaccggc atgggcggtt tcccggaagg
     3001 ttgggcgcca ggtgaacact atcccgaaaa atgggcgcgt cgttttgccg tcgatttcgt
     3061 tggtggtgat ctgaaagctg ccgcgccaaa aggcattgag ccggtgatta cgctttccag
     3121 tggggaagcg aagcaaatcg aaattctcta tattgaaccc attgatggtt atcgtattca
     3181 gtttgactgg tatccgactt cggactccac tgatccggtc gatatgcgga tgtatctgcg
     3241 ttgtcagggc gacgctatca gtgaaacatg gctctatcag tatttcccgc cagcgccgga
     3301 taaacgtcag tatgttgacg accgcgtgat gagttagtcg ttttttcttc ggcaccttct
     3361 tcgggaggtg ccgtctggtt aaacacgatc ccgctcgcat ttttccctaa gttaaatgag
     3421 taatctgatg gtgtgtattt cagatacacc ttgtcagcca ctaacaggga gtgcgtatgt
     3481 ttccagaata ccgagattta atatcccgtc tgaaaaacga aaatcctcgc tttatgtcct
     3541 tgttcgataa acacaataaa cttgatcatg aaattgccag aaaggaaggt tccgacggtc
     3601 gagggtacaa tgcggaagtg gtccgcatga aaaaacaaaa gctacagtta aaagatgaga
     3661 tgctcaaaat cctgcagcag gagagcgtca aagaggtgta aactttccta agccgccagc
     3721 caggcggctt ttttaacaac tgcatggatt gactggagat aagatgactg aaacgataaa
     3781 agtaagcgaa tcacttgaat tacatgctgt tgcagaaaat cacgtcacac ctctttatca
     3841 gttaatctgt aaaaataaaa cctggttaca gcagtcgcta aactggccgc agtttgttca
     3901 aagtgaagag gacacgcgaa aaacggtgca gggtaatgtg atgttgcatc aacgcggcta
     3961 tgccaaaatg ttcatgattt tcaaagaaga tgaacttatc ggcgttatct cgtttaatcg
     4021 tattgaacca ctgaataaaa ccgctgaaat tggctactgg ctggacgaat cttatcaggg
     4081 gcaggggatc atttctcagg cgctgcaggc attgattcat cattacgctc agtctggtga
     4141 acttagacgc ttcgtgatca aatgtcgggt ggacaatgcg caaagcaacc aggtcgcttt
     4201 gcgcaatggt tttatccttg aaggttgtct gaaacaggct gagttcctga atgatgccta
     4261 tgatgatgtg aacttatacg cgcgtattat cgattcacaa taaccctgcc agcggcgttc
     4321 gcgtaatgcg ctcctcgccg ttaatgactt ttggaccacg gacatgaacc gtgtcgccat
     4381 caaaggcttc aacgacggcg tggtcggtta actccacatg tttttcaatt atcaccgcac
     4441 ctgtaatacg gctctcacct tgtattacaa cgtgctcgtc gagcagaatc ggtccgccac
     4501 gtaccacggc atttccgcca atcaagacat gatgttttaa cacacaatta ccttccacaa
     4561 tggcatattc cgccacctgc gaactgtaat gaatcgtcgg aatggcatct tcttctatgc
     4621 cagctttcac ctgcgcgtga ccgtagactt tagcgcaatc gcatagccag acgttgttct
     4681 cttcattacc ttcaacgctg gcaaaatcaa aaacttcggc gcgatgttca ataaaagcat
     4741 accggacgac ggcatcgcca taaatttgtg cctggtgaac aatacgcgag gcgctaaccc
     4801 ttgcgcgatc ataaatttgt agcaggagtt gatggtcggg cgttaagcct tgtgcggcga
     4861 cgatcataga atgttgatca attaaggcgt gaccaaatat tctacattga ccacaaacta
     4921 atgagtcacg aatggtgacg ctgtcactta tatatgcacc ttggctgatt tcactattat
     4981 cgatccagac attatccgtt gcgtaaacct ctccccacag cacgctggtg ccggtgatac
     5041 gcgtattccc ggatatcacc gttccgccga acgcaatagc attctggtcg taaatccagc
     5101 aatttccttc ctgtgctaac accgtttcgc gatcgatcca gccgccagcg gtttctgcta
     5161 tcacatcgtt aaaatcgctc atggcgataa tctgccgtaa taacacactt tttttagtgc
     5221 catcatcctg ataactaaag gctcgctgtt cttcacttag acgatatttg cgcatagcgt
     5281 tttcccacag gtgacttact ataaccgtag caaattctgc ggctctggct atgctcgaga
     5341 aaattccata aaatgcattt caaata
//
LOCUS       DAPHCV010000195         5344 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.197, whole genome shotgun sequence.
ACCESSION   DAPHCV010000195 DAPHCV010000000
VERSION     DAPHCV010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.197"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(290..481)
                     /locus_tag="RH033_004470"
     CDS             complement(290..481)
                     /locus_tag="RH033_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HDV2993552.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            545..868
                     /locus_tag="RH033_004471"
     CDS             545..868
                     /locus_tag="RH033_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HDV2993553.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     ARDDS"
     gene            810..1568
                     /locus_tag="RH033_004472"
                     /pseudo
     CDS             810..1568
                     /locus_tag="RH033_004472"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1546..2064
                     /locus_tag="RH033_004473"
     CDS             1546..2064
                     /locus_tag="RH033_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbeB"
                     /protein_id="HDV2993554.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSN
                     ETKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTA
                     SGGTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVI
                     PFQGKEWVHLKE"
     gene            2071..2304
                     /locus_tag="RH033_004474"
     CDS             2071..2304
                     /locus_tag="RH033_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HDV2993555.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
     gene            complement(2622..3041)
                     /locus_tag="RH033_004475"
     CDS             complement(2622..3041)
                     /locus_tag="RH033_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HDV2993556.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGF
                     DPNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGT
                     TQFAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
     gene            complement(3122..3559)
                     /locus_tag="RH033_004476"
     CDS             complement(3122..3559)
                     /locus_tag="RH033_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HDV2993557.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGR
                     REFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDK
                     QAQDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
     gene            3819..>5344
                     /gene="cea"
                     /locus_tag="RH033_004477"
     CDS             3819..>5344
                     /gene="cea"
                     /locus_tag="RH033_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="HDV2993558.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSK
                     SESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEAL
                     RHNASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEI
                     EREKAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSR
                     LSSSIHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRR
                     RVGAGKIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLK
                     KAQNNLLNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAA
                     FEKYKDVLNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSD
                     ILKIKDTGDWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYI
                     "
BASE COUNT         1416 a         1151 c         1496 g         1281 t
ORIGIN      
        1 cgaacgaccg agcgtagcga gtcagtgagc gaggaagcgg aaaagcgcct ggacgtgcat
       61 tttctcctta cgcatctgtg cggcatttca cacccggcat ggcgtacttt tcatacaatc
      121 cgcactgatg ccgcatggtt aagccagtat acactccgct atcgctacgt gactgggtca
      181 gggctgcgcc ccgacacccg ctaaaacctg ctgacgcgcc ctgacgggct tgtcagctcc
      241 cggcatccgc tcacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
      301 caccgtcatc cccgaaacgt gcgaggcagc tgcggtaaag ctcatcggcg tggtcgtgaa
      361 gcgattcaca aatatcggcc tgttcatctg cgtccagttc gttgagcttc tccagcagcg
      421 ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttagtca
      481 ctgatgcctc cgtgtaaggg ggatttctgt tcatggggta atgataccga tgaaacgcga
      541 gaggatgctc acaatacggg ttactgatga tgaacatgcc cggttacttg aacgctgtga
      601 gggtaaacaa ctggcggtat ggatgcggcg ggtctgcctg ggggagccgg ttgcccgttc
      661 cggaaaactg ccgacactgg caccgccgtt actgcgtcag ctggccgcca tcggaaataa
      721 cctgaatcag acagcccgta aggtgaacag cgggcagtgg tcttccggtg accgggttca
      781 ggtggtggcc gcactgatgg ccatcgggga tgagctgcgc cggctgcgtc tggctgtcag
      841 ggaacagggg gcgcgggatg atagttaaat ttcatgccag gggaaaaggt ggtggcagtg
      901 gtccggttga ttacctgctg gggagggagc gtaaccgcga aggcgcaacg gtgcttcagg
      961 gtaatccgga agaagtccgg gaactcatcg atgccacgcc atttgcgaag aaatacacgt
     1021 ccggtgttct gtcgttcgcg gagaaggagc tgccgccggg aggacgtgaa aaagtgatgg
     1081 cgagctttga gcgtgtactg atgcccggtc tcgaaaaaaa tcagtacagc atcctgtggg
     1141 tggagcacca ggacaaggga cggcttgagc tgaattttgt cattccgaac atggagctac
     1201 agaccggaaa acgcctccag ccgtactacg accgcgcaga caggcctaga attgatgcct
     1261 ggcagacgct ggtaaatcac cattacgggc tgcatgaccc gaacgccccg gagaaccgca
     1321 ggacgctgac actccctgat aacctgcctg aaacgaaaca ggcgcttgct gagggcgtca
     1381 cgcgaggtat agatgcactt taccatgccg gagagataaa aggccgtcag gatgtgattc
     1441 aggcgctcac tgaggcgggg ctggaagtgg tcagggtgac gcgaagcagt atcagcattg
     1501 cagatccgaa cggcgggaag aatatcaggc tgaaaggagc attttatgag caatcttttg
     1561 cagacgggcg cggagttcga gaaaaagctg aaagagagag ccgaatctac agagaaaatg
     1621 ctgaacaacg agttcaggag gctcggcgaa tctgtaagcg aggctgtgac atcaaacgag
     1681 acgaaaatca gagacgctat agccctgttc acagcctcga cagaggaatc gctggaaaaa
     1741 caccgggaag gggtgaaaga ggcgatgatg cagcacagga gggacgtgtt aaagctggca
     1801 gggaatacgg gcatgatgtt actggggata gtctttctcc tgtttaccgc gagtggcggg
     1861 acgctctggt atcttggagg gaggatacag gcgaacctgg aagaaatcag gaagcaggaa
     1921 gagacattgc agaaactgaa cgcgaagaca tggggcgtgg agtttgtgca ggacgggaac
     1981 aggaaattcc ttgtccttcc gtacgggaaa tcagcggagg tgattccctt tcaggggaaa
     2041 gagtgggtac atctgaagga gtgacacaga gtgacagagc tggaaacaca tttgctgaac
     2101 gccttagagc agctgcaaca ggactatatg cagcggctga gcgaatggga gagcgccttc
     2161 gtggaattgc agaagatgtt ttcgcttacg caacgggaca acgcgatgct gaacgagcgg
     2221 gtcatgcagt tgagtcagca ggtgcagcac ttgagcgagc agacagaacg cttgagccag
     2281 ttatacagcg agaactggag ataagagagg aacggctgat acaggagcgc gaacatgtgt
     2341 tatccctgga acgggagcgt cagccggaaa tacaggaacg cacgctggat ggcccttcgc
     2401 tgggatggtg aaaccatgaa aaatggcagc ttcagtggat taagtggggg taatgtggcc
     2461 tgtaccctct ggttgcatag gtattcatac ggttaaaatt tatcaggcgc gatcgcggca
     2521 gtttttcggg tggtttgttg ccatttttac ctgtctgctg ccgtgatcgc gctgaacgcg
     2581 ttttagcggt gcgtacaatt aagggattat ggtaaatcca cttactgtct gccctcgtag
     2641 ccatcgagat aaaccgcaga aatcgtgtca gccagcagcc tggatttttt cggggtaagg
     2701 tctttgctgc ggtaatcagt acccgcaaat tgagttgtgc cccggagtga gtttaaaaat
     2761 tcctggctgt gaaactgctg aatgcgttgt tgcacatcac tccgtgattt tcctgtcgct
     2821 ttatctttag ctcccatatc ccagaactgc cgaaaaaaag gcaccatcat tcgtgttgtg
     2881 tcggcttttt gggtggaaaa gttgggatca aaaccgtcat cagaagcata tacgtaatgc
     2941 cgggtatgtt gttccacgct gccctttggg gtacaggctg tgagagctac tgttgctgtt
     3001 atcatggcga taagacaaag tttgtttccg gtacgtctca tgagtgtctc cctaccttaa
     3061 agtatttatt ctcggtgagt tattatccga agactatttt atcatcagtc tctgacagcg
     3121 ttcagcgacc gaacaacctg gaccagaatc cccgtttctt ttccagttcc agtgcctgcc
     3181 tgagctgggc tatctcattt tgtagctgtt cccgttctgc ttcctggcgt ctgcgatcca
     3241 tatcctgtgc ctgtttatcc tcaagcatca gcgtcaggca ttgtttcagc tcattcagtt
     3301 cccggagaat gcgttctgtc tgctgatcat gtggattttc tgcatgtccc tcactgtgcc
     3361 tttctggtgt ctcattctgc tttaattcgc cgtatgcccg gatcagttca ctggtttcaa
     3421 actcccgtcg tccgtccctg ccggtacggt aactgacaag gcctgcgcgc atatcacggt
     3481 aaaactgtga acgcgatctg cctgtcattt ttagtgcgtc ccggagtgtg tgccatgcca
     3541 taaagtgaca gtgtcccata gatgtctcat ctcatagttt cagtaaaaca taatgaggtc
     3601 tgagaacggt aatgtttgtg ctggtttttg tggcatcggg cgagaatagc gcgtggtgtg
     3661 aaagactgtt tttttgatcg ttttcacaaa aatggaagtc cacagtcttg acagggaaaa
     3721 tgcagcggcg tagcttttat gctgtatata aaaccagtgg ttatatgtac agtatttatt
     3781 tttaacttat tgttttaaaa gtcaaagagg attttataat ggaaaccgcg gtagcgtact
     3841 ataaagatgg tgttccttat gatgataagg gacaggtaat tattactctt ttgaatggta
     3901 ctcctgacgg gagtggctct ggcggcggag gtggaaaagg aggcagtaaa agtgaaagtt
     3961 ctgcagctat tcatgcaact gctaaatggt ctactgctca attaaagaaa acacaggcag
     4021 agcaggctgc ccgggcaaaa gctgcagcgg aagcacaggc gaaagcaaag gcaaacaggg
     4081 atgcgctgac tcagcgcctg aaggatatcg tgaatgaggc tcttcgtcac aatgcctcac
     4141 gtacgccttc agcaacagag cttgctcatg ctaataatgc agctatgcag gcggaagcag
     4201 agcgtttgcg ccttgcgaaa gcagaagaaa aagcccgtaa agaagcggaa gcagcagaaa
     4261 aggcttttca ggaagcagaa caacgacgta aagagattga acgggagaag gctgaaacag
     4321 aacgccagtt gaaactggct gaagctgaag agaaacgact ggctgcattg agtgaagaag
     4381 ctaaagctgt tgagatcgcc caaaaaaaac tttctgctgc acaatctgaa gtggtgaaaa
     4441 tggatggaga gattaagact ctcaattctc gtttaagctc cagtatccat gcccgtgatg
     4501 cagaaatgaa aacgctcgct ggaaaacgaa atgaactggc tcaggcatcc gctaaatata
     4561 aagaactgga tgagctggtc aaaaaactat caccaagagc caatgatccg cttcagaacc
     4621 gtcctttttt tgaagcaacc agacgacggg ttggggccgg taagattaga gaagaaaaac
     4681 aaaaacaggt aacagcatca gaaacacgta ttaaccggat aaatgctgat ataactcaga
     4741 tccagaaggc tatttctcag gtcagtaata atcgtaatgc cggtatcgct cgtgttcatg
     4801 aagctgaaga aaatttgaaa aaagcacaga ataatctcct taattcacag attaaggatg
     4861 ctgttgatgc aacagttagc ttttatcaaa cgctgactga aaaatatggt gaaaaatatt
     4921 cgaaaatggc acaggaactt gctgataagt ctaaaggtaa gaaaatcggc aatgtgaatg
     4981 aagctctcgc tgcttttgaa aaatacaagg atgttttaaa taagaaattc agcaaagccg
     5041 atcgtgatgc tatttttaat gcgttggcat cggtgaagta tgatgactgg gctaaacatt
     5101 tagatcagtt tgccaagtac ttgaagatta cggggcatgt ttcttttgga tatgatgtgg
     5161 tatctgatat cctaaaaatt aaggatacag gtgactggaa gccactattt cttacattag
     5221 agaagaaagc tgcagatgca ggggtgagtt atgttgttgc tttacttttt agcttgcttg
     5281 ctggaactac attaggtatt tggggtattg ctattgttac aggaattcta tgctcctata
     5341 ttga
//
LOCUS       DAPHCV010000196         5166 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.198, whole genome shotgun sequence.
ACCESSION   DAPHCV010000196 DAPHCV010000000
VERSION     DAPHCV010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.198"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(237..1259)
                     /locus_tag="RH033_004478"
     CDS             complement(237..1259)
                     /locus_tag="RH033_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HDV2993559.1"
                     /translation="MDKRVIFAVAGSGKTTLLIRRLREDRRTLILTFTVNNEAHLRAQ
                     IIRRFGYIPYGIRVMTWFEFLHGFCFRPFLQEQLSSRGLSFNQPPSRIPRTNIRHYQD
                     PAGRLYHRRLAHLLTARGLLPDIRTRLARYYDELFVDEVQDFAGHDFNFLLELCRAEI
                     SVLCCGDFYQHTFDTSRDGNVNATLHEDITRYEARFRAAGIMVDCETLSRTWRCSATV
                     CEFITGQLNIRISAHGTHTTQIEIVTDEARSAALHADNTMIKLFYREHHRYGCHSMNW
                     GGSKGLDHFQDVCIVMGANHWMRLIQQKLAALPPSSRNRLYVACSRARGNIYFIPESH
                     LRRFRN"
     gene            complement(1244..2809)
                     /locus_tag="RH033_004479"
     CDS             complement(1244..2809)
                     /locus_tag="RH033_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDV2993560.1"
                     /translation="MPLITRLMLQNFKKFPELDLRFSNDRNILVGDNESGKSTILLAL
                     DLVLSDSRHRVEALGVESLLSQSAVRHFQEGERRADQLPVLTADVFLSNGGEPDLNGR
                     QNLAGIDADGLRMRIAPMMEEYGQDIHHVLQQDPDNFPYEYYSVQFSTFSGGHFAGFR
                     RYLRHLFLDSARIDNEHAAQEYTRTVYSVNVPVADRYRLENSYRQQKMHFCARHLSAI
                     NDTLKTYQFGVRSGAKSGLEANLDITEDGISIRHRGKGRQCFIKTEFALQRHQQQGGE
                     IHALLLEEPENHLSHVSMKRLVNQLATERQTQVFIATHSSHISSRLDLRKAILLGATR
                     PVLMNELSAETAAFFMKAPDNNVLEFALARRVLLVEGDAEFILIEAFYHRLYGRAPED
                     DGVHIIAIGGTSFRRYLELARLLENRVAALRDNDGNYQQNCDERYADVLCSRSRVFAD
                     HDNSRSTFEICLYQDNADLCDALFRGTRRTLTVQDYMLANKAEAAFQLLQLHAEKLTV
                     PDYIQEALAWIRE"
     gene            complement(2884..3300)
                     /locus_tag="RH033_004480"
     CDS             complement(2884..3300)
                     /locus_tag="RH033_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HDV2993561.1"
                     /translation="MNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITY
                     SEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRAAVDATTEIKVALRLAGTPIGPN
                     DTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR"
     gene            complement(3297..3527)
                     /locus_tag="RH033_004481"
     CDS             complement(3297..3527)
                     /locus_tag="RH033_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDV2993562.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVR
                     PTWGSFLELEKADPDFMTEREDVVSDEGRVNL"
     gene            3908..>5166
                     /locus_tag="RH033_004482"
     CDS             3908..>5166
                     /locus_tag="RH033_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pcar"
                     /protein_id="HDV2993563.1"
                     /translation="MPVLSANKAQEEQINTSYEPLTPSVITDEKAQSYIEALNFACSR
                     PDIRNIAVTGPYGAGKSSVLLTWERAEDNDFRVMTVSLADFEMQRAYPGDSLSVEGKP
                     DYDDNEKKAGKAEEKTIEYSILQQLLYKEKKSVLPYSRLERISDVSACQIAMMTASLL
                     FILASTATGLLFLFPDYIREKLSLSPELSQFLLELPVLARFGSAGIFLFTALFFALKK
                     LHRTGVFDRRVSIDKVDILKGAITTRPAAPSLLNVYIDEIVYFFEQTQYNVVIFEDLD
                     RHNDGAIFIKLREINQLINNCLPTDNPVRFIYAVRDNLFITPESRTKFFDFVIPVIPV
                     MDSENASEHFLSKFTLDELRQEGFKDCLARLALFIPDMRVMHNIANEFRLYRNIVNNG
                     GDLKRLISLITYKNLYAEDYHRIDQKKG"
BASE COUNT         1256 a         1314 c         1299 g         1297 t
ORIGIN      
        1 gcttcctggg gggttaatgg ctgatgacga taaaatcata tatcgaatga tttatttctc
       61 tgcctgtaaa tatcaggcca ggatttatta tgcgattaat gataaatatc atatcatctg
      121 cgggtttccc tccacgcagg tccggtttcc ggcgaaaccg gagcctcatt tttcggcctg
      181 aagttttgtc gttgactact ttttaccggc ttgtgcccag ttgagaagcc gctgacttag
      241 tttctgaatc tccgaagatg gctttccgga ataaaataga tgttgccccg tgcccgtgag
      301 caggccacgt atagcctgtt acgggatgat ggtggaagtg ctgccagctt ctgttggatg
      361 aggcgcatcc agtggttggc ccccatcacg atgcagacgt cctgaaagtg gtcgagtcct
      421 ttactgcccc cccagttcat tgagtggcat ccgtaacggt gatgttcgcg atagaagagc
      481 ttgatcatcg tgttgtccgc gtgcagggcc gctgaacgcg cttcatccgt gacgatttca
      541 atctgggtgg tatgtgttcc atgagccgaa atgcgaatgt tcagttgtcc ggtaatgaac
      601 tcacacactg tagctgaaca gcgccatgtc cggctgagcg tctcacaatc caccatgata
      661 cccgcagccc tgaagcgagc ctcgtatcgg gttatgtctt catgcagcgt agcattgacg
      721 ttaccgtcgc ggcttgtgtc aaaggtatgc tgataaaaat ccccgcagca cagcactgag
      781 atttccgcgc ggcatagctc cagcagaaaa ttaaagtcat ggccggcaaa gtcctggact
      841 tcatcaacga acaactcatc gtagtaacgg gcaagtcggg tacggatatc tggcagcaac
      901 ccccgggctg tcagcaggtg tgcgagccgc cggtgataca gccgtccggc gggatcctga
      961 taatgccgga tattcgtgcg cggtattctg gaagggggct ggttgaagct cagaccacgc
     1021 gatgacagtt gctcctgcag gaaagggcga aaacagaaac cgtgcagaaa ctcaaaccag
     1081 gtcatcacac ggatcccgta cggtatgtag ccaaagcgtc tgataatctg cgcccgcaga
     1141 tgtgcttcat tatttacggt gaaggtcagg atcagcgtcc gccggtcctc tcttaaccga
     1201 cgtatcagca gcgtggtttt acccgagccg gcaaccgcaa atatcactct cttatccatg
     1261 ccagtgcctc ctggatataa tcaggaacgg tcagcttttc ggcgtgcaac tgtaacagct
     1321 ggaatgctgc ttctgcctta ttggccagca tgtaatcctg aaccgtgagt gtccggcggg
     1381 ttccgcggaa aagcgcatca cacaggtcgg cattatcctg atacaggcag atttcaaatg
     1441 tggagcggct gttgtcgtgg tcagcaaata cccgggagcg ggaacaaagc acgtcagcat
     1501 agcgttcatc gcaattttgc tgatagttgc cgtcattgtc gcgaagggcc gcgacacgat
     1561 tttccagaag acgggccagt tcaagataac gacggaaact cgttccgccg atagcgatga
     1621 tgtgaacccc gtcatcctcc ggtgccctgc catacaggcg gtgataaaag gcctcgatca
     1681 gaataaactc agcatccccc tccacgagca gcacgcgtct ggccagcgca aattccagca
     1741 cgttattatc cggtgctttc atgaagaagg cggcagtctc agcggaaagt tcattcatca
     1801 gtaccggccg ggtagctccg aggagaatag ccttacgtag gtcgaggcgg gaggagatat
     1861 ggctactgtg tgtggcaata aacacctgcg tctgccgttc cgtggcaagc tgattaacca
     1921 gcctcttcat actgacatgg ctgagatgat tctccggctc ctcaagcaac agcgcgtgga
     1981 tctctccccc ctgctgctga tgccgctgca gcgcaaactc cgttttgatg aaacactgtc
     2041 ttccttttcc gcggtgacga atggaaatac cgtcctcagt aatgtccagg ttcgcctcaa
     2101 gacctgattt agcgccggaa cgcacgccaa actgatacgt tttcagcgtg tcgtttatgg
     2161 cggacagatg ccgggcgcag aagtgcattt tctgttgccg gtaactgttt tcaagccggt
     2221 accgatcggc caccggcaca ttgacgctgt aaactgtgcg cgtatactcc tgtgcggcat
     2281 gctcgttatc aatgcgtgca ctgtctaaga acagatggcg cagatagcgt ctgaagcctg
     2341 cgaaatgacc accggagaat gtgctgaact gcacggaata gtattcatag ggaaaattat
     2401 caggatcctg ctgcaaaaca tggtgtatat cctgaccgta ttcctccatc atgggagcaa
     2461 tgcgcattct gagaccatcc gcatcgatac ccgccagatt ttgcctgccg ttcaggtcag
     2521 gctctccccc attactcagg aaaacgtcag cggtcagcac gggtaactga tcggcacggc
     2581 gctcaccttc ctgaaaatgc ctgactgctg actgtgacag gagcgattcc acccccagcg
     2641 cctcaacccg atgcctgctg tcactcagca caaggtcaag agccagcaga atagtgcttt
     2701 tcccggactc attgtcccca accagaatgt tacgatcgtt gctgaagcgc aggtccagtt
     2761 ccggaaactt tttgaagttt tgcagcataa gccgcgtgat cagtggcatt tttttattcc
     2821 tttatatacc gctgcctgaa agagcggccg gccgcgcctg accgcctgtt ttgttttaca
     2881 gcgttatctt acccagtctt ccagcacaag acccggtacc cgctcaaact ctctcacatt
     2941 attcgtcacc agaatggcac aggcagcgat ggcgtgtccg gctatcgccg tgtcgttcgg
     3001 accgatcggc gtgccggcga gacgcagtgc caccttaatc tccgtggtcg catccaccgc
     3061 ggcgcggtcc cacggcagga cggcatcgag cctctcacag aacgcgtcaa ccagttgcac
     3121 gtggcgtggc gaggccttcg gaccggtggc accgaagcgc atctcggagt aggtgatggc
     3181 cgagaccacg atacggtgac cgcgcagcac cgactgctcc aggtgcttaa gcaatgcttc
     3241 aggttgctcg cgcatgatga acgagcagat gttcgtgtcg agcatataga tcttgttcac
     3301 aggttaactc gtccttcgtc gctgacaacg tcctcgcgct ccgtcataaa gtccggatcg
     3361 gccttttcta gctccaggaa tgagccccag gtcggccgga cggggcgcag aatgatgctg
     3421 tccccttccc ggacgatctc cagctcgctc accccctcaa aatccagatc gcgtggaagg
     3481 cggatagcac ggttgttgcc atttttaaaa atggatacgg ttctcatgat tctctcccgg
     3541 gtaagtgtgg atatgtgatg catatccgga atgatgttca ttgcaccggg tgtgtgcata
     3601 tgtttagtat aggcagatgt taggcatatg tatagattca gcatatgcag atggtgagga
     3661 tatgcgtata ccatgaatat gcagatgtca ggcatatgga tatgttcagc ctatgttatt
     3721 ttctctcttt ccccgtgcag atccggtttc tggtgaagtc ggagcccacc atctccgagt
     3781 atttgataag ccaattcttg tatccctttg cctttattat ctttttggtt ttcagaagtt
     3841 ataatgcctg ttaaatcaac aatgttggaa gatcaacatg agaagaccat cccgaactga
     3901 agcactgatg ccagtactga gtgctaacaa agctcaggaa gaacagatta acacctccta
     3961 tgaaccactg actccctcag tcattacaga tgaaaaagca caaagctata tcgaagcttt
     4021 gaattttgcc tgttctcgcc cggatatcag aaatattgca gtaacagggc cgtacggggc
     4081 cggtaaaagt tcggtattac tgacatggga aagggcggag gataatgact ttcgggtgat
     4141 gaccgtatca ctggctgact ttgaaatgca gcgggcatac cccggagatt cgctgtcagt
     4201 tgagggtaag cccgactatg atgataacga aaaaaaagcg ggaaaagccg aagagaaaac
     4261 aatcgagtac agcatcctgc aacaactgct ctataaagag aaaaaaagtg tgttacccta
     4321 ttcccgtctg gagcgcattt ctgacgtaag tgcgtgtcag atagccatga tgacggccag
     4381 tctgttattc atactggctt cgacggcaac tggtctgctt tttcttttcc cggattacat
     4441 ccgggaaaag ctctccctgt cgccagaact gagtcagttt ctgctggagt tgcctgtact
     4501 cgctaggttt ggtagcgccg ggatatttct ttttactgct ttattctttg cactgaagaa
     4561 attgcaccgt acaggggtat ttgatcggcg tgtaagtatt gataaagttg acatactgaa
     4621 aggtgccatt acaacacgcc ctgcggcccc ttctcttctc aatgtgtaca ttgatgaaat
     4681 cgtctacttt ttcgaacaga cacaatacaa tgtagtcata tttgaggatc ttgaccgtca
     4741 taatgacgga gccattttta tcaaactccg ggaaataaat cagcttatca ataactgcct
     4801 ccccacagac aatcctgtcc gatttatcta cgctgtcagg gataatcttt tcataacacc
     4861 agaatcacgg acaaaatttt tcgatttcgt cataccagta atcccagtaa tggacagtga
     4921 gaatgcttct gagcattttc taagtaaatt tacacttgat gaactcaggc aggaaggatt
     4981 caaagactgc cttgccagac tggctctttt tattcctgat atgcgggtta tgcataacat
     5041 tgcgaatgaa tttcgtcttt accggaatat tgtgaataat ggaggagatt taaagcgtct
     5101 tatttcactg atcacctata aaaaccttta tgctgaagac tatcacagga tagatcagaa
     5161 aaaagg
//
LOCUS       DAPHCV010000197         5093 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.199, whole genome shotgun sequence.
ACCESSION   DAPHCV010000197 DAPHCV010000000
VERSION     DAPHCV010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.199"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            73..765
                     /locus_tag="RH033_004483"
     CDS             73..765
                     /locus_tag="RH033_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDV2993564.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQK
                     TMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQYA
                     KGEAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYELLQQFRSTGERI
                     IALNDFRSMLGIENKYKQFRDLNKILIKPCITELNKKSDLVVTVETIKKGRTVAALHF
                     RFKEDKQIKMTI"
     gene            complement(1298..1789)
                     /locus_tag="RH033_004484"
     CDS             complement(1298..1789)
                     /locus_tag="RH033_004484"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993565.1"
                     /translation="MAIGDDAIRDAFYLFTQQVAEMDNESLPKDVWGTPCFTYLMTRK
                     QFNQMKVICQRNGWDVPTSPAIPITWSMFKHVLSARKSKDKLSWQECAEILATAFSVQ
                     SNVYVSRDYSEQTIVLNASCRISVAGAGFFAMAIIDVSENNLAPVTAYHVTEAKCKAI
                     SRG"
     gene            complement(2042..4201)
                     /locus_tag="RH033_004485"
     CDS             complement(2042..4201)
                     /locus_tag="RH033_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HDV2993566.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTR
                     KSGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAAR
                     ETVLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTH
                     AEAWRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETE
                     RGEQNRLINRLNLEIQISRTELRNGGLTEPARRVANLLNIGLPDNATSYTLRDIIEAL
                     PKDSNAAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRI
                     PLMQWAAPEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWAASGL
                     AALRAQLLKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAP
                     MPGNWEMTCYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHP
                     PLQRVYSSATFEQLLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVD
                     FTQGRLPPTLMPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPTADNS
                     QLVINALTDKGLRFAGYGEAVNHDADSPARPAPELMLFHLKTREAPLFAAVNTPEKQP
                     DHLFRSLGFNRTWDEWQREEYARTRVSDRKHERGWLL"
     gene            4380..4634
                     /locus_tag="RH033_004486"
     CDS             4380..4634
                     /locus_tag="RH033_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HDV2993567.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRK
                     PEEVQLRAWLLPFLRREVTRQADLNRIQPLIDELEALPER"
BASE COUNT         1271 a         1208 c         1264 g         1350 t
ORIGIN      
        1 aaaaaaaaat attccacatt tcttatttgt ggggcacatt aaagtatcac cacatttgaa
       61 cgggagcttt gtatgtcgga cttgattgcc tacaagtcga atgctctagt tgaggccagc
      121 tacaaactga ccctacagga acaacgtttt ctgttgctgt gtatcagtcg attgaaatct
      181 ggtgctgatg ctgaattgca aaaaacaatg accattaccg ccgcggaata ttttgattca
      241 ttccctgaca tggggcggaa aaatgccgag gttcagttac aggaagcgat cgatcgttta
      301 tgggacaggt cgatcattct caaggatgac gagaagcgtg aggagttccg ctggatccag
      361 tatcgcgcac agtacgctaa aggtgaggcc aaggctcgaa taaccttctc tgacgcggta
      421 atgccttatc tgacgcaact gaaggggcaa ttcacgcgcg ttgttataaa aaacatatca
      481 ggtttatcaa gcagttacag tataagaata tacgaacttc tccagcaatt ccgcagtacg
      541 ggagagagaa ttattgctct taatgacttt cgttcaatgt taggtattga aaataaatac
      601 aaacaattca gagacttaaa taagatacta ataaaaccct gtatcacgga actgaataaa
      661 aaaagcgatc tggttgtgac ggttgagacg atcaagaagg ggcgcacggt tgcagcactg
      721 cattttcgat tcaaggaaga caagcagatc aagatgacga tctaaaatcg attttaaggc
      781 atctaaaacg cgcgctggcg cattttttta catctggtaa tggtggcgat ggaagagagg
      841 ggaaaactgc gtaaaaataa ctttagagag ctttaaagca tctttagcgt ttgggttcca
      901 gttggaccat agtcattact ctacgctaac aaccagtacc aatcggataa aactaattga
      961 ggcagatgag gcccctaagt cacaaaatgc gacattccac accctatctt aagcccctgt
     1021 tcgcggggag tttagcctta gctgcgctca ataaatattg tactatagcc acaattgatg
     1081 tagattgaat gtactgcaag aacattccat aaacggagaa atcctctaat gttcggcagt
     1141 gggagcattg atgttcctga acaattagcg atttgataga cagcagaaaa ccgccgatgc
     1201 tgctagatcc gaaaggaaac aatgaaggga aatatttagg cgttatggtt cattctcatt
     1261 aaattgagga gagctgctta aatgggcgtc cttcctatca ccctcgcgat atagctttac
     1321 atttggcctc cgtcacatga tatgcagtta cgggggccaa attattttca gagacatcaa
     1381 taatcgccat tgcgaaaaat cctgcccctg caacgctgat gcgacaagaa gcattaagta
     1441 caatcgtctg ctctgaataa tctctgctaa cgtatacatt actctgcaca gaaaaagcag
     1501 ttgccaagat ttctgcgcat tcttgccagc ttaatttatc cttcgatttt cgagcagaga
     1561 gcacatgctt gaacatactc caagtgatag gtatcgccgg tgatgtgggc acatcccaac
     1621 cattcctctg acaaatgacc ttcatttggt taaattgttt tctagtcatc aaataagtga
     1681 aacaaggtgt tccccagacg tctttaggga gtgattcatt atccatttca gcgacttgct
     1741 gtgtaaaaag ataaaaagca tcacggatag catcatcgcc aatagccact gattttcctt
     1801 atttatccgg cattttttag ttatgacggt cttcacgcgt cgttattcca ctgccgacag
     1861 ctcagcataa ttcaatgtct gagcaatcgc tttattatca tacctgaaac ggctattgcg
     1921 cggtctttct cccgtttgcc gaatcacggt cgttgcgcta cctactagag ttgaaatttt
     1981 ctatctcaaa acgaattatt ctgttttcgc ccatataaaa aatatgtatg cggaaagtgc
     2041 tttaaagtaa ccagccgcgc tcatgtttcc tgtcggagac gcgtgttcgt gcatattcct
     2101 cgcgctgcca ttcgtcccag gtgcgattaa aacccagcga gcggaacaaa tgatccggct
     2161 gtttttctgg tgtattgacg gcagcaaaaa gcggtgcctc acgggttttc agatgaaaca
     2221 gcataagttc tggcgcaggg cgtgccggac tgtcagcatc gtggtttacc gcttcaccat
     2281 aaccggcaaa tcgtaaacct ttgtcggtca gagcattgat taccagctgg ctgttgtcgg
     2341 ctgtggggac ggcaaacgca ggatacagcg taccattaag ccggacaaca ccgcccagag
     2401 agtcatccac gcccatgacc agcaaaagct gaagctggcg ggtttcccgg tcgacagaac
     2461 tcattaccgg catcagcgtc ggtggcagac gtccctgcgt aaaatccacc ttccttgctg
     2521 ccagtgatgg catggctggc tggcggtctt ctccaagaca aaaagactcc ggccatgtct
     2581 tcggctggcg gagattgcgg gtatgttgcg gccatgtctg gcaaacctgc gtcagaagtt
     2641 gctcaaaggt cgcagaggaa taaacacgtt gcagtggtgg atgttgaccg atttcgcttc
     2701 ggtcacagat ataaccactc ctgtcacgca ttaaatcaac acgctcaaag atcctggtgc
     2761 ggtatttctc caactcgttc ccccagagcg tatattctcc gttccggttg tgaagcagaa
     2821 ggtaacaggt catttcccag ttaccaggca taggggcctc gccatacccc gtaacgactg
     2881 cgctcagtac ccctgaatcc ataacggcat tgtcattttt cctgagctgt tccgctcgtc
     2941 gctccgcgcg actacgttct gtatcctcct gctccttcct gcgtaattcc tcttcacgtt
     3001 taagcaactg ggctctgaga gctgcaaggc cggatgcggc ccactgctgc cactgttttt
     3061 caaatgccgt ctgactcgcg ggaatgtcct gtttctccac cttccggtaa tgctgtcgca
     3121 atttttccat ttgctggctc agagactgga tttttccctg ctcactacgg tattccggtg
     3181 ccgcccattg catcagggga atacgggaaa aaccggacga aaacggcgat gattttttca
     3241 ggctggcagc atggctcatc gcttcctttc gttcggtgtt cagctcttcc cagcgtctgc
     3301 gcgtcgcctg catatctgcc atcatcgaca tggcattact ggtgagtttc catgccgcat
     3361 tactgtcctt cggtaacgct tcgatgatgt cacgtagtgt gtacgaagtt gcgttgtcag
     3421 gaagcccgat attcagaagg ttagccacac ggcgggctgg ctctgtcagc ccgccgttcc
     3481 tgagttcggt gcgggaaatc tgtatttcca ggttaagcct gttaatcaga cgattctgtt
     3541 cgccgcgttc tgtttcgata ccacgtgttt ccatcgcaca ggcagacttt ccgaggtgaa
     3601 tacccggcgt tttctcaagc ccctgtcgct cgtatgaacg atggtctatc tcttcctgat
     3661 aaccggcgtt cgccagtgcc ctgtttgcat ggtcagccca tgatgcccgc cacgcctcag
     3721 catgtgtccg gtcgttccag tccctgacct ttccgccgaa tccagcaggt ccgacagccc
     3781 tcgtggtcag catgatgtgc gcatggggat ttgttttatc catgtgatgg aacgccacat
     3841 cagcaatcat gccctgactg acaaagtttt ctctgacaaa ggcgagaacg gtttcccgtg
     3901 cggcatcctg cggcagttcg cgggggatgg cgagttcaat ttcccgcgca agttgtgagt
     3961 tcttccggcg ttcggccttc tcaacggcat tccagagttc ggcacggttc gtacaccagg
     4021 caggggctgt cgcgggggtc agaatcacag cttcagccac accgcttttg cgggtgtagt
     4081 catgagtcag tcctgtacgc tcatcaagca tcagtgaacc ggaacgatag gcagcagaag
     4141 cgacggcgct gtagccgctg tttctggaga tgattttcat gctgagatga taaatcgcca
     4201 tttctgccgt aatgcctttt tgtcgttttc tttcagcccc gcagggcgca cacaatcgaa
     4261 acggagtgga gattgtataa gtgcgcactt cgtgatgtgc cgtgcattat gccagttcag
     4321 tttttaaact gccagtgacg cgataaaata cgatttttca tcgcgcacgg agttacggaa
     4381 tgagcagaac actcgaacag aagattgccg aagctgaggc cagattgcag cgcctgaagg
     4441 cgaagagcag gagtctggac acagcgcaga aggtcgttgt gggtgcagca ttgctggcaa
     4501 aggtcagaaa gccggaggaa gtgcagttgc gggcctggtt gctcccgttc ctgcggagag
     4561 aagttacacg gcaggctgac ttgaacagga tacagccact gattgacgag ctggaggcat
     4621 tgccagaacg gtgattacac cgtccgctcg ccgcagctcc agcgaccaag cgttagcgag
     4681 cgagtgagcg aggaagcgga agtgaacccg tagccaggtt gagcacatac gcacggtttt
     4741 tcggcaggtg atacgcgctg aacccgcatg aatactggat ttccacaggg ctggaggggg
     4801 agtattcgga atttgcaatt ctgctttttt gcaacgcatt gtttttactg agtttttctt
     4861 tttggcatcc taagttttcc acaggctcgc cgttaagttt ttagatcttg tttttaagat
     4921 cttgttttta atacttgttt taggggtgaa attcattatt aaaatcaatg gcttacagtt
     4981 gtataagtgg agggatttaa ggtaaaggtg gaggaattta aggtgtttag gtggagggat
     5041 ttaaggtaaa ggtggaggaa tttaaggtgt tattcacaga aatgtgagga tat
//
LOCUS       DAPHCV010000198         5059 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.200, whole genome shotgun sequence.
ACCESSION   DAPHCV010000198 DAPHCV010000000
VERSION     DAPHCV010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.200"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..400
                     /locus_tag="RH033_004487"
     CDS             <1..400
                     /locus_tag="RH033_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009447271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prepilin-type cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDV2993568.1"
                     /translation="SSTMASSDNQRELKNLQTIATKMKQYKFQGRYTGTDYIKTLTES
                     GALPADMIAGGNKAKNAWGGDVTIAATADKYGYIITSNNVPKTNCVELINSLRSSSIF
                     TKIMNTAPNMVDPVTVCSSDKNNIKLETNS"
     gene            454..927
                     /locus_tag="RH033_004488"
     CDS             454..927
                     /locus_tag="RH033_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDV2993569.1"
                     /translation="MKWFYLSSLFLFCLPATAAVNSNTLDSCFIQAGKRYSIDPGLLK
                     VIARKESSLNPRAINHNRNASGKIISTDYGLMQINSTHIPGLKRMGIIRSENELLSNV
                     CLNIQTGAWILARHFQQCGVNWECLGSYNAGFNKNNTHRRMKYARLIYAAYMQGR"
     gene            946..1581
                     /locus_tag="RH033_004489"
     CDS             946..1581
                     /locus_tag="RH033_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDV2993570.1"
                     /translation="MFLIILLVISATLTIQLLAIRLAGHYLMQVHTLRRPRHLSLLCA
                     VLALTATITHIIRGTDIAGLCFLLLMAGCMTLMTLTDAASCRLPRPFTLTFLMLGVAF
                     RLWQGDAAIALACALFWFLLLSLLRRYTSYRKGVETLGLGDVFLIAGVAMWSSANSTP
                     WIIGIAAASTMICMSLFPRSNTGAAIPFGPFICASLYIFTFFPGAIFHTGG"
     gene            1586..2950
                     /gene="pilV"
                     /locus_tag="RH033_004490"
     CDS             1586..2950
                     /gene="pilV"
                     /locus_tag="RH033_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HDV2993571.1"
                     /translation="MQKDDDKGFVLFEILAGLIVIGIATPMIYSEIENWLNEQLYQSA
                     AYHADAYNTAARNYIADNNARLHNGSLPANFTADDLIRQGYLKQGFNHSPFGQSYITG
                     IRRNQTSGRLEALTCSTGGQTIKEEGLRSVAGQLPGLGGFISKNGTATGAFGAWTDKP
                     GDYGLTCSAGHIAVVMSGDDLQESDRLYRFQVAGRPELNQMHTAINMGGNNINNTGNI
                     NGQSATLKGDITSEDGWLITRNNKGWMNITHGGGFTMTDSQWIRAVNGKGITTTGEIK
                     GGKVSGGTVRSDGRLSTGEYLQLDKTATAGTKCAPDGLVGRTSTGAILSCQSGMWAGF
                     ESYPVGSPIPWPSATPPQGYLVMNGQSFSCSRYPQLARAYPGCKLPDLRGVFIRGWDN
                     GRGLDGDRNRHLLSYQADQSGVYHERGGWLKGHHSGMAYWAQGSTTEMRPKNIAFNYI
                     VKAS"
     gene            3565..4734
                     /locus_tag="RH033_004491"
     CDS             3565..4734
                     /locus_tag="RH033_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDV2993572.1"
                     /translation="MFQKNKIRKMSLSRALDKYLKTVSVHKKGHLQEFYRVNVIKRHP
                     MADRYMDEITTIDIAGYRDQRLAQINPRTGRQITGNTVRLELALLSSLFNIARVEWGT
                     CRMNPVELVRKPKISNGRDRRLTSGEERRISRYFRDKNPQLYVIFHLALETAMRQGEI
                     LSLRWEHLDLQHGVAHLPETKNGSPRDVPLSRKARNYLQILPQQINGNVFSYTSSGFK
                     SAWRTALLDLKIENLHFHDLRHEAISRLFELGTLNVMEIAAISGHRSLNMLKRYTHLR
                     AYHLVSKLDARRKQTSKISPYFVPYPATVRNRNGVFIVTLHDFDLESRAETRELAISH
                     ASVLLLRALAQAAQRGERVPTPGELPANIDERVMICPLTNSQFMTKANSIFISSS"
     gene            4776..>5059
                     /locus_tag="RH033_004492"
     CDS             4776..>5059
                     /locus_tag="RH033_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993573.1"
                     /translation="MKHLSSRAATADNNRIVTIEDVYTIAFSQPIHTESALGQRWMLI
                     HTGEYESASDAKRFLDTLVTGHCACMVTPSPVLDHDTWLRIRLLMGEIRDA"
BASE COUNT         1341 a         1321 c         1177 g         1220 t
ORIGIN      
        1 gtccagcaca atggcctcca gtgataacca gcgcgaactg aaaaacctgc agacaattgc
       61 gaccaaaatg aagcaataca agttccaggg aagatatacc ggaacggatt atatcaaaac
      121 cctgacggaa agcggtgcat tgcctgctga tatgattgca ggaggcaaca aagccaaaaa
      181 cgcatggggt ggtgatgtca cgattgctgc cacagcagac aaatatggct acattatcac
      241 cagtaataat gttcctaaaa caaactgtgt tgagctgatt aacagcctgc gcagttcgtc
      301 catatttacc aaaattatga acacagcccc gaacatggtg gatcccgtta ccgtctgcag
      361 tagcgataaa aacaatatca agctggaaac caacagctaa tgccctgcca cacagcactc
      421 cctccggggt gctgtttccc ggagcacata atcatgaaat ggttttatct ctcatctctt
      481 ttcttattct gtctcccggc gactgccgcc gtaaacagta acacactgga tagctgcttt
      541 attcaggcag gaaaacgtta ctccatcgat cccggactac tgaaagtaat tgcccgtaaa
      601 gaaagcagcc tgaatccccg ggccatcaat cataacagaa atgccagcgg aaaaattatc
      661 agcacagatt atggcctgat gcagattaat tccacacata ttcccggact gaaaagaatg
      721 ggaattatcc gaagtgaaaa tgaactgctc agtaatgttt gcctgaatat acagacaggt
      781 gcatggatcc ttgcccggca ttttcagcag tgtggtgtga actgggagtg tctcggtagc
      841 tacaacgccg gatttaataa aaataacact caccgcagaa tgaaatatgc ccggctgatt
      901 tatgccgcat atatgcaggg acgttaataa cggagaatac gagccatgtt cctcatcatc
      961 ttactcgtga tctccgctac gctgaccata cagttacttg ccatcagact ggccggacat
     1021 tatctcatgc aggtgcatac cctccggcgt ccacgtcacc tctccctgct ctgtgctgtt
     1081 ctggcactca ccgccaccat tacgcacatc atacggggga cagacattgc cggactttgc
     1141 tttctgttgc tgatggccgg ctgcatgaca ctgatgacac tgactgatgc agcctcctgc
     1201 cgtcttcccc gtcccttcac cctcacattc ctgatgctgg gtgtggcgtt tcgtttatgg
     1261 cagggcgatg ctgctattgc gcttgcctgt gctctgttct ggtttctcct gttatctctc
     1321 ctgaggcgct acacctcata ccgcaaggga gtggagacgc tgggactggg ggacgtgttt
     1381 ctgattgcag gtgtggccat gtggtcatct gccaacagca ctccgtggat catcggtatt
     1441 gccgccgcca gcacaatgat ctgtatgtcg ctgttcccgc gcagtaatac tggggccgct
     1501 attccgttcg gtccgtttat ctgtgcatca ctctacattt tcactttttt tcctggtgcc
     1561 atttttcaca ccggagggta acaccatgca aaaagacgac gataaaggtt ttgttctgtt
     1621 tgaaattctg gccggactga ttgtgatcgg tatcgccaca ccaatgattt attcagaaat
     1681 tgaaaactgg ctgaatgagc agctttacca gagtgctgca tatcatgcag atgcctacaa
     1741 taccgcagcc agaaactata ttgccgacaa caatgcccgt ctgcataacg gcagccttcc
     1801 ggcaaatttc acggctgatg acctgattcg tcagggatat ctgaaacagg ggtttaacca
     1861 ttctccgttt ggtcagagtt atatcaccgg tatccgccgt aaccagacct ccgggcgtct
     1921 ggaagccctg acctgctcaa ctggagggca gacaatcaaa gaagaagggc tgcgcagcgt
     1981 ggcgggtcag ttgccggggc tgggtggatt tatcagcaaa aacggaacgg caacaggcgc
     2041 attcggagcc tggacagata aaccgggaga ctacggactt acctgttcgg caggtcacat
     2101 cgccgtcgtc atgagcggtg atgacctgca ggaaagtgac cgtctctacc gttttcaggt
     2161 agcaggtcgt cctgaactga atcagatgca taccgccatc aatatgggcg ggaataacat
     2221 aaacaacacc ggcaatatca atggtcagag tgccacactg aaaggtgata ttacgagtga
     2281 agacggctgg ctaatcacca gaaacaataa gggatggatg aatatcacac atggtggtgg
     2341 attcaccatg acagacagcc agtggatacg cgcggttaat ggcaaaggca taacaaccac
     2401 cggggaaatc aaaggcggaa aagtcagtgg tggtacagtc cggtctgacg gtcgtctttc
     2461 aaccggggag tatttacagc ttgataaaac agctacggca ggcacaaagt gtgcaccgga
     2521 cggacttgta ggccgcacca gtacaggggc catactttcg tgccaatccg gtatgtgggc
     2581 aggttttgaa tcctaccctg tcggaagccc tataccgtgg ccatcagcca cccctccaca
     2641 aggctacctg gtgatgaacg ggcagtcatt ttcatgttcc cggtatcctc agcttgcccg
     2701 tgcttatccc gggtgtaagc tgccggattt gcgcggtgtt tttatccgtg gctgggacaa
     2761 cggacgtggg ctggatggag atcggaacag acatctcctg agttatcagg ccgatcaatc
     2821 cggtgtctat catgagcgtg ggggatggct gaaagggcat cacagcggta tggcttactg
     2881 ggcccaggga agcacaacgg aaatgcgccc caaaaacatt gcctttaact acatcgtcaa
     2941 agcaagctga tgcatttaag gcgtattaac ttttaactta cagcaacgta ttttgaccca
     3001 gaccatgctt cccgaatacc ccggtttacc tctgtacgct ctgaatcctg ccgcaccata
     3061 gcctgctttg ccgtcacaga cggcaaagcc gtttgagcca ctaccccagt tcgcataaac
     3121 cggttttctg gagtcttctt ctgcgtcata acaggccgaa taatcaaaaa ccagtgcatc
     3181 gctgatatta ttcttccaga caccggattg gcacgctggc tactgcctga cgcttaatta
     3241 aataccggcc ccaggtatat tgagacacgt aacagatatc gttccacctg actcataatt
     3301 accccacgca taattattga cataccagac attattttta tcggaataaa cctgctgaaa
     3361 accaccgaaa ggagaaccat taagatacgt ccatgcacaa tatgcatgga caccaagtct
     3421 gaaattacga atattttttc cgatgttata ggtctgcgtt gtgaatttaa tttcagttga
     3481 ccgccagaca ccggattggc acgaaagtat tgcatttctg tatccagcct cctaccttcc
     3541 ccgtttttat cgtggaaggc agatatgttt caaaaaaaca aaattcgcaa aatgtccctc
     3601 agccgggcac tggacaaata cctgaagact gtttccgtcc ataaaaaagg tcatctgcag
     3661 gagttttatc gcgtcaatgt gattaaacgt caccctatgg ccgatcgcta tatggatgaa
     3721 atcactacca ttgatatcgc cggttaccgg gatcagcgtt tagctcaaat taacccacga
     3781 acagggcgtc aaatcaccgg aaataccgtc cgtcttgaac ttgccctttt atcatcatta
     3841 ttcaatatcg cccgagtgga atggggaaca tgccgtatga atcctgttga actggtccga
     3901 aaaccgaaaa tcagtaatgg ccgcgaccgc cgactgactt ctggtgaaga acgccgcatt
     3961 tcccgctatt tcagggacaa aaatccgcag ctctacgtta tcttccatct ggctctggaa
     4021 acagcaatgc gccagggaga aattctctca ttacgctggg aacacctcga tttgcagcac
     4081 ggcgtggcgc atcttcctga aacgaaaaat ggctcaccac gcgacgtacc actctcccgg
     4141 aaagcacgta actacctgca aattctgcca caacagataa acggcaatgt gttcagttac
     4201 acctcgtccg gtttcaaaag cgcctggcgt accgctcttc tggatctgaa aatagaaaat
     4261 ctgcattttc acgatctccg ccatgaggcc atcagccgtc tgttcgagct cggcacactg
     4321 aacgttatgg agattgcagc catttccggt caccgctccc tgaacatgtt gaaacggtac
     4381 acacatctcc gggcatatca cctggtcagt aaactcgatg caaggcgtaa acagaccagc
     4441 aaaatttccc cttattttgt tccctaccct gcaactgtca gaaacagaaa cggagtgttc
     4501 attgtgacac tgcacgattt cgaccttgaa agccgtgctg aaacccggga actggcaata
     4561 tctcacgcca gcgtattgtt gctgcgcgca ctggcccagg ctgctcagcg gggtgaacgc
     4621 gtccccacac caggcgagct acccgcaaac attgatgaac gggtcatgat ttgtccgtta
     4681 acaaacagcc agttcatgac aaaagcgaac agtattttca tatcctcctc ctgaacaaat
     4741 aatttttgtt caaaattaac gctatgagac cctttatgaa acacttatcc tcccgtgcag
     4801 ccactgcgga taataatcgt atcgttacaa ttgaagacgt ttacacaatc gcattctcgc
     4861 aaccaattca caccgaatca gcgttggggc aaaggtggat gttgatccat actggtgaat
     4921 atgaaagtgc cagcgatgca aaacgcttcc ttgatacgct cgtcaccggg cactgtgctt
     4981 gcatggtaac accatcacct gttctggatc atgacacctg gctgcgtatc cgcctgctga
     5041 tgggcgagat aagggatgc
//
LOCUS       DAPHCV010000199         5030 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.201, whole genome shotgun sequence.
ACCESSION   DAPHCV010000199 DAPHCV010000000
VERSION     DAPHCV010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5030
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.201"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(64..822)
                     /locus_tag="RH033_004493"
     CDS             complement(64..822)
                     /locus_tag="RH033_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDV2993574.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEDLALHYLERVRIAEHAQKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="RH033_004494"
     CDS             complement(830..1933)
                     /locus_tag="RH033_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDV2993575.1"
                     /translation="MTKVLLSHPPRPASHNSSRAMVWVRKNLFSSLSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..3124)
                     /locus_tag="RH033_004495"
     CDS             complement(1943..3124)
                     /locus_tag="RH033_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDV2993576.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQIFAVVAVVGIVGWLFHNTV
                     TNLSNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPRPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILTVVIAIAISVGLFRFNKTYQIKTGQLRRTWP
                     VAVVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3192..4217)
                     /locus_tag="RH033_004496"
     CDS             complement(3192..4217)
                     /locus_tag="RH033_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV2993577.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGVVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(4648..4869)
                     /locus_tag="RH033_004497"
     CDS             complement(4648..4869)
                     /locus_tag="RH033_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993578.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
BASE COUNT         1405 a         1290 c         1173 g         1162 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg atcacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaactcatcc ggtgcagctt gttccactat ctccccacga tccataaaaa tcaccctgtc
      181 agcgacggtt cgtgcaaacc ccatctcatg tgttacacac agcattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tcaggatcga gcgccgacgt
      301 tggctcatca aataacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccaccagaaa tctgtccggg aaacttctgg gcatgttcgg caattctcac
      421 ccgctcaagg taatgcaacg ccagatcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtagaac ggtcagatga gggaagagat tgaaatgctg
      541 aaagaccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatcc accacgatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgcccatac catttattga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaga
      901 agatccagta gatcagtgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caacccgatg atgatcacca
     1021 ggctggtatc tttgaaaagt gcgatgatgg tatttaccag cccaggaatc accagcttca
     1081 acgcctgtgg cagaataacc agcccctggg ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccgcc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaaaggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa cgccagtaag atcccccacg gtaacgcccc cgcaatccca actgatgcaa
     1441 taattaaagt tagcgtcagc ccaccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat cactgcccag accgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgacaca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagcccatac ataaactgac
     1681 caaatcgttc gtggatgaag acccaacagg caccggcttt tgtgcagtcg gcacgcgttg
     1741 agccaaccca gttagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagtaggc tattgctcaa gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcggggga tgagacagca
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgcccagtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat agcggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aatcactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggcaatcc cagcgaacgt gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagcgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc atcccgccac ggaagttaaa gcctcgtagg gctggaacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacct atgatcaaca
     2461 caacggcgac cggccaggtt cggcgcaatt gtccggtctt tatctggtac gttttattaa
     2521 aacggaatag tccaacagaa atggctatag caataacaac agtcaggata aacgcaataa
     2581 atccgtctcc cagctgcggc gatggaatat aaagcccacg attgctcaaa aaggccagat
     2641 caaacgcact cactgcctgg cgtggtctgg gcaaattgcg caacacggca aagtaccaga
     2701 agaagatttg cagcagcggc ggaatattac ggaagatctc aatataaatt gtggaaagct
     2761 ttcgtagcag ccagttatcc gaaagtcgcg ccagaccgat aaagaagccc agaacagaag
     2821 cgaatacaat acacaatgca gaaaccagta gcgtattgag taagccaaca atgaaaacgc
     2881 gtccgtaggt atcaccctgc tggtaatcga tcaaatgctg gacaataccg aagccagcgc
     2941 cgcgatccag aaaggcaaaa cctgaagtaa tgccacgatt actgagattc gtcacagtgt
     3001 tgtgaaatag ccaaccaaca attccgacaa cagcaacaac ggcaaagatc tggaataacc
     3061 aggcgcgaac cgtagggttg gcaaaggaga gtgagccttt aacggttgag cggcgatgag
     3121 acatagaaac ctcggaaagc ataacgctga agagagcgcc gccgtagcga cgctcgttac
     3181 ctcacaccga cttaacgcac gggcggcgcg tactgaattc cgccgttatt ccagagatta
     3241 ttttgcccac gtttaatttt cagcgggctt tctgaaccta cgttacgctc aaaaatttcc
     3301 gagtagttac ccacctgttt gatgatgttg taagcccatt tattatccag cttcagatcc
     3361 ttgccgtaat cgccttcttt acccagcaga tgtgccatat caggcgttgc tggattagcc
     3421 gctttttcat cgacgttctg ggaattgatg cccatctctt cagcattcag catggcgaaa
     3481 agcgtccagc gtacaatcga gaaccattca tcatcgccac gacgaactac cggcccaagc
     3541 ggttctttgg agataacttc cggtaagaca atccattcag ccgggttgct taatttgatg
     3601 cgcagggcat acagttgtga ttgatccgag gccagcgtat cgcagcgacc agattccagt
     3661 gccttcgctg attcgtcaga gcgatcgaaa gtcactggtg tgtacttcat attgtttgcc
     3721 ttaaagtaat cggcaacgtt gagttcggta tcagtacccg cctgaataca gacggtagcg
     3781 ccatccagtt ctttcgcgct ttttagtccc gctttatcgt gcgtcaggaa gccaatgccg
     3841 tcgtaataag tgacgccggt aaatgccatt cccatcccgg catcgcgaga tgaagtccag
     3901 gtcgtattac gggagagcaa atccacctcc cctgactgta aagcggtgaa gcgttctttt
     3961 gcagtgagcg gggtatattt cactttcgtg tcgtcaccaa atacagctgc cgcaacacca
     4021 cgacaaacat ctacatcaat acctgaaaac ttaccgtcag catcggcata agagaaccca
     4081 ggtaatccat cactgatccc gcattgcaca accccttttt tctgaacggc atcaagcgtc
     4141 gcgccagcat gcgcctgatt tgcaacggca agcagcacgc tggcggcagc cagtgtggct
     4201 atcatcatct ttttcataat gcttcctgtg aggcgaaaat tatcgttata ggacgtttgg
     4261 gaaacgtctt ttacctgtct gtggccatcc tcttataagc aaaagtagtg ccagcttttg
     4321 cgcacgattt cgcgtcgcaa taagctgagt gtaggcagga aataataaaa actgcgcccc
     4381 gcaatggtgc gaaaatgaaa catgcgccac attttggcgc aaaatattta cgcgtttttt
     4441 actaagcaga ataatgattg ttagcgtgag tatatttctg gtgggatgtg tgaaagttaa
     4501 attgatgcgt tatttcgaaa gcagtgaaat aacgtgatat aacatgctaa atcagcaggg
     4561 aattacccag cagaatatag caatatttta tcttatggaa agatactgta tcgttaaaaa
     4621 gagcgcggag ctccgcgccc tgtttagtta tttcgacaga gcgatgatcc ccatcgtcag
     4681 gccagcaaca gctgctgccc cgccggcaat agccccagcc gtttcccagt tatcctgagt
     4741 cgtgcctttc atacaggtat tggtgtgaac cagacgaccc tgatcgtcat aaaccggaac
     4801 gcagggcgaa tcgtgcgcac agcccgccag caatgcggtg agcaatgcca caggaattaa
     4861 tcttttcatg gtgttacctc agtaataaac gctaacgctt aataagttaa ctctcttatt
     4921 aacgagctga atgccatttt atcactggag gttataaatc ttgcggaatg aataatgaca
     4981 aattatgtga attgtaaaaa tagtgaaggg agcgggaact atagaaagtt
//
LOCUS       DAPHCV010000200         5020 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.202, whole genome shotgun sequence.
ACCESSION   DAPHCV010000200 DAPHCV010000000
VERSION     DAPHCV010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.202"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            153..905
                     /locus_tag="RH033_004498"
     CDS             153..905
                     /locus_tag="RH033_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDV2993579.1"
                     /translation="MIKIYKASKGQLKHREIFDEIKKKVDNGEWKEGKAIPTERELSE
                     YYLSSRTTIRKAIESLKQRGYLHSVHGQGTFVLPSRSRENHLLHSFTDDIKARGGIPA
                     QNILEIGYVPVSDVIRKNLDLDIHVQTVFCIKRVRYMGSTPLGIQTSWLALNDRQEIT
                     QEDLLATGSLYILLEEKLAIKPLEATEIISARLPSPLERQLLELTADDVVLSCTRVTL
                     SVERKPMEYVEMVYPASRYSYKARITKDSFSV"
     gene            919..1995
                     /locus_tag="RH033_004499"
     CDS             919..1995
                     /locus_tag="RH033_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HDV2993580.1"
                     /translation="MNKIEQAVIYNKILGSLACAGMGDALGAATELYSIDEIKAQWGG
                     FLNAFVSPPADTFAGSLNGIAGLITDDSSQMYVFSEGLIEAGFDNFTNNDWLACLLRW
                     ADMQPYANYKGPTTEQIVKALKEGRPTNTIGRIGTSSRQAPNVGTTNGAGMRVAPAGL
                     IWPGKKEKACHLALITCLPSHDTNIAIASACAIAAATSQAMLPEASLTSLLDAAIWGA
                     NYGERLAKQYARCVAGPSIAMRIQLAADIARRANDLESCLREMEGLVGNSVAAHESIP
                     AAIGLLLYCKGEPWETIHACANIGNDTDSIATMAGAIAGAWRGFDALPEDKYAFFRAV
                     NNKDFDIEAIASGLTLLALEAQEK"
     gene            1995..2993
                     /locus_tag="RH033_004500"
     CDS             1995..2993
                     /locus_tag="RH033_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDV2993581.1"
                     /translation="MNMPARTPVMPLAQTREEINATAASLRHLAAIWGECFSTFQLDK
                     RAVERVCIVGSGDSWTVALCAAAWLGKYTHLFCYALQTWDFLQTDLTRYQKETLIIIL
                     SASGRPSMTVDALCHAVCSNAQVLGVTNCPGTPFCAITENMLYTWANKQGIPTQSSSV
                     TLYALLRLAQKLCPGLTPLQIEDDLEGKFSQINQHWQQKKRHFYQQKEITFLGSGLSW
                     GLAISGSNLLSCGPQIRATALPLEEFYHSLRLHQAGVGQHYILLPATSCESPFYLATQ
                     KAIVEQGATAELISFIPDASESNNLFLVMQWLYEMCWHLSCDYVDAGGQRVSHREK"
     gene            2998..3912
                     /locus_tag="RH033_004501"
     CDS             2998..3912
                     /locus_tag="RH033_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HDV2993582.1"
                     /translation="MKKFDVAALGSGNIDIFLSIPSLPTRGGKVVGTRLGEQAGGTVA
                     NSACAMGQLGLNVVSVSCVGNDHSASIILDGFKKYHVNCDFVQVIPELIANTAIIFID
                     ELGEKTLVYSPGSDHEWDKEKALQAIAQSRYFYTMPADIEKFRMLAEYSRSQMTKVVV
                     DIEPHIIATPEHLARILHLADIAIFNYDGFIRGYATEPDFTLLHDIQDEYQLDAVVVT
                     LDVRGVIAVKGNEQVKIGSYTIPVIDTTGAGDTFNGAFVYSLIKNMPLIEALKFASAT
                     AAINITALGARGHLPTPSEVNLFLSQHD"
     gene            3940..>5020
                     /locus_tag="RH033_004502"
     CDS             3940..>5020
                     /locus_tag="RH033_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HDV2993583.1"
                     /translation="MKIRYAAIFLALPLLCTTAQANSFLHYAGEFNFRTGEKQNNVTI
                     AGLSAITFDAKKNIFYAINDSRNNNKEGDASLYTLKIQVSSRGIEQVNFLNQRPLLDA
                     KQQPFVTNTVDAEGLALTHNGKSLLWSSELGTPLRLSTLDGVMEKDFTSLFPARFNIS
                     SDKESSNGIRSGNAWEGLTVTPDGKSLFIAVESSLKQDGPIASPINSGTSRLLQFSID
                     ADGRPSKQLHEYLYITDPVPQVSKFGINDNGVSEVMALNDHQLLVIERSGRNVSAGFN
                     DWDYSVRVYMVDLTAASDIKNIDSLQDWSNKSTLQPVSKKLLIDFADYTSSADCIEGV
                     TFGPLIDGHTSLIFVSDNNFQPHQQT"
BASE COUNT         1472 a         1061 c         1132 g         1355 t
ORIGIN      
        1 atcacataaa aaccaatttc acttaatctc atgatttata tatttaattg tggttttttc
       61 tgttttatgt cacagatagt gaacaagcac catgcgtaat aaaagttaaa gaatgtagag
      121 ttggtatcaa ccacttgaaa tgaggctttg tcatgataaa aatttataaa gccagtaaag
      181 gtcagctaaa gcatcgtgaa atctttgatg aaataaagaa aaaagtagat aacggtgaat
      241 ggaaagaagg caaagccata ccgacagaaa gggaattgtc agaatattat ttaagtagca
      301 gaacaactat tcgaaaggca atagaaagtc ttaaacaacg cggctacctg catagtgtcc
      361 acgggcaagg aacatttgta ttgccttctc gtagtagaga aaatcattta ttgcatagtt
      421 ttaccgacga tattaaagct cgaggtggta taccagcaca gaatattctt gagataggct
      481 atgttcctgt cagtgatgtt atcagaaaaa atctcgacct ggatatccat gtccaaaccg
      541 tattctgtat aaaacgcgtt cgctatatgg gaagtacccc cctgggtatt cagacatcat
      601 ggcttgcatt aaatgacagg caagaaataa cccaagaaga tctgctggcg acgggttcgc
      661 tctatatttt actggaagaa aaactcgcta ttaaaccttt agaagcaaca gaaattatct
      721 ctgcccggct tccctcgccc cttgagcgtc aattactgga actgactgcc gatgatgtcg
      781 tattaagttg tacccgcgtc acgttatccg tggaacgcaa accaatggag tatgtagaga
      841 tggtgtatcc cgcaagccgg tactcctaca aagcacgaat tacaaaagac agttttagcg
      901 tgtaacagag agaatattgt gaacaaaata gaacaagctg taatttataa caaaatatta
      961 ggttctttag cctgcgcagg tatgggcgat gcattaggcg cagcgactga actctacagc
     1021 attgatgaaa taaaagctca atggggaggt tttttaaatg cctttgtttc tcctcctgcc
     1081 gatacattcg ctggctcatt aaatggtatt gcgggactta tcaccgacga ttccagccaa
     1141 atgtatgtgt tttcagaagg gttaattgag gctggatttg ataacttcac caataatgac
     1201 tggttagctt gtcttctgcg ttgggccgat atgcagccct acgcaaatta taaaggcccc
     1261 acaacagaac aaatcgtcaa ggcactaaaa gaaggtagac caacaaacac cattgggcgt
     1321 attggaacat cttctcgtca ggcacccaat gtcggcacca cgaatggagc aggaatgcgt
     1381 gtagcccctg caggattgat ctggccaggc aagaaagaaa aagcgtgtca cctggcgcta
     1441 ataacttgcc taccttcgca tgacaccaat attgcgattg ccagcgcttg cgccatcgct
     1501 gcggcgacat cacaagctat gttaccagaa gcttcgctca catcattact ggacgccgca
     1561 atctggggag cgaattacgg cgaacgcctg gccaaacaat atgcacgatg tgtcgcaggg
     1621 ccatctatcg ccatgcgcat ccaacttgca gcggacatcg ccagacgagc caatgacctg
     1681 gagtcttgtc tgcgagaaat ggaaggtttg gttggtaact ctgttgccgc ccatgaatcc
     1741 attcctgcgg caatcggtct attgctgtac tgcaaaggag aaccctggga aaccattcat
     1801 gcctgcgcca atattggcaa tgacaccgat agcatcgcta cgatggcagg cgcaattgca
     1861 ggggcatggc gcggttttga tgcgctccct gaagataaat atgccttttt ccgcgccgta
     1921 aataataaag atttcgatat tgaagcgatc gcatcgggtc tgacattgct tgcattagag
     1981 gcacaggaga aataatgaac atgccagccc gtactcctgt tatgccatta gcgcaaactc
     2041 gtgaggagat aaatgctacg gcggcatcgt tacgccattt ggcagctatc tggggtgaat
     2101 gtttttctac ttttcagctc gataaacgtg ctgttgaacg cgtctgtata gtgggatcgg
     2161 gcgactcctg gacggtggca ctatgtgccg ccgcctggct ggggaaatac acacatctgt
     2221 tttgttacgc attgcaaacg tgggattttc ttcagactga tttaacccgt tatcagaaag
     2281 aaacgttaat cattattctc agtgcaagtg gcagaccatc aatgaccgtt gacgccctct
     2341 gccacgcagt atgctcaaac gcgcaagtgc tgggtgtgac gaattgccct ggaacaccat
     2401 tttgcgcaat aacagaaaac atgctctata catgggcgaa taagcagggt attccaacgc
     2461 aaagtagtag cgtcacactt tatgccctcc tgcgcttagc gcaaaaatta tgtccaggct
     2521 taacaccact gcagattgaa gatgatttgg aagggaaatt tagccagatt aatcagcact
     2581 ggcagcaaaa gaagcgccat ttttaccaac aaaaagagat aacgtttctt gggagtggct
     2641 taagttgggg actggcaata tcggggagca atttactttc ttgcggccca cagattcgtg
     2701 cgacagccct tccactggaa gaattttatc attcattacg cttacatcag gctggtgtcg
     2761 gtcagcatta tattttacta ccagccacat cctgcgagtc cccattttac cttgcaaccc
     2821 aaaaagcaat tgttgaacag ggagcgacag cagaattaat cagttttata cctgacgcca
     2881 gtgagtccaa caatttattt ctggtcatgc agtggttata tgaaatgtgc tggcatttaa
     2941 gttgtgatta tgtcgacgca ggcgggcaac gcgttagcca tagggagaaa taagactatg
     3001 aagaaatttg atgtcgccgc tttaggcagt ggaaatatag atattttttt atctatcccg
     3061 tcactcccca ctcgtggcgg taaagttgtc gggacacgtc tgggtgaaca agctggcggc
     3121 acagtcgcca acagtgcctg tgcgatggga caactgggtc tcaatgtggt atctgtttca
     3181 tgtgtgggca atgatcattc agcctcgatc attcttgatg gatttaaaaa atatcatgta
     3241 aattgtgatt tcgttcaggt aattccggaa ttgattgcca ataccgcgat tatctttatt
     3301 gatgaattag gtgaaaaaac gttggtttac tcgcctggta gtgatcatga gtgggataaa
     3361 gaaaaagccc tgcaggcaat tgcgcaaagc cgttattttt atacgatgcc agcagatatc
     3421 gagaaatttc gtatgctggc cgaatattcc cgtagccaga tgacaaaagt tgtcgttgat
     3481 attgagcctc atatcattgc aacacctgaa caccttgcca gaattttaca ccttgctgat
     3541 attgcgatat ttaattatga cggttttatt cgtggctatg caacagagcc agattttaca
     3601 cttctgcatg atatacaaga cgagtatcaa cttgacgctg tcgtggttac gctggatgtt
     3661 aggggagtaa ttgcagtcaa aggcaatgag caagtaaaaa taggtagtta tacaattccc
     3721 gttattgata ccacaggcgc tggagatact tttaatggtg catttgttta tagcttaata
     3781 aaaaatatgc ccttaattga agcactaaaa tttgcttctg ccacagcagc aataaatatt
     3841 actgcattag gtgccagagg tcatctgccg acaccatcgg aggttaatct ttttctttca
     3901 caacacgact aaaatcatat atatacaaag gtaaatatca tgaaaataag atatgccgct
     3961 atttttctgg cattaccgct gttatgcaca acagcgcagg caaacagttt tcttcattat
     4021 gctggggagt ttaacttccg gacgggagaa aagcagaaca atgtcacaat cgcggggcta
     4081 tcagccatca catttgatgc aaaaaaaaat attttctatg ctattaacga tagcagaaac
     4141 aataataaag aaggagatgc cagtctttat acactaaaaa tacaggtatc atccaggggc
     4201 attgagcaag tgaattttct caaccagcgt ccactgcttg atgcaaaaca gcaacctttc
     4261 gtgaccaata cggtggatgc cgaaggtctg gccctgacac acaatggtaa atcattactc
     4321 tggagttcgg agttaggtac tccactgcgt ctgagcactc tcgatggcgt aatggagaaa
     4381 gattttacgt ctttatttcc tgctcgcttt aatattagtt ctgacaaaga gagcagcaat
     4441 ggtattcgca gcggcaacgc ctgggagggc ctgacagtta caccagatgg aaaatcactt
     4501 tttatcgccg ttgaaagcag ccttaaacag gatgggccta ttgcaagtcc aataaattca
     4561 ggcacatcac gcttactgca attttctata gatgcagacg gtcgaccttc aaagcaattg
     4621 catgaatatc tctatattac tgatcctgtt ccgcaagtga gtaagttcgg tattaatgat
     4681 aatggcgtat ccgaggtcat ggcgctgaat gatcaccaat tgttggtcat cgaacgttcc
     4741 ggcagaaacg tcagtgctgg gttcaatgac tgggattact ctgtcagagt ttatatggtc
     4801 gatttaactg ctgccagcga tatcaagaat attgacagtc tgcaagattg gtccaataaa
     4861 tccacgttac aacccgtcag taaaaaatta ttaattgatt ttgctgatta cacttcctct
     4921 gcggattgta ttgaaggggt aaccttcggt cctctgattg atgggcatac atcgttaata
     4981 tttgtatctg ataataattt ccagccccat caacagacga
//
LOCUS       DAPHCV010000201         4996 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.203, whole genome shotgun sequence.
ACCESSION   DAPHCV010000201 DAPHCV010000000
VERSION     DAPHCV010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.203"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..721
                     /locus_tag="RH033_004503"
     CDS             <1..721
                     /locus_tag="RH033_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="APH(3')-I family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HDV2993584.1"
                     /translation="VGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLT
                     EFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSI
                     PVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDS
                     VVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQK
                     YGIDNPDMNKLQFHLMLDEFF"
     gene            complement(881..1468)
                     /locus_tag="RH033_004504"
                     /pseudo
     CDS             complement(881..1468)
                     /locus_tag="RH033_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743064.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C"
     gene            complement(1455..2282)
                     /locus_tag="RH033_004505"
     CDS             complement(1455..2282)
                     /locus_tag="RH033_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDV2993585.1"
                     /translation="MALDLMAAFTELPPPIDYVLPNMVAGTVGALVSPGGAGKSMLAL
                     QLAAQIAGGPDLLEIGEFPTGQVVYLPAEDPPAAIHHRLHALGAHLSAAERQAVADGL
                     LIEPLIGKCPNIMAASWFDALKRAAEGRRLMILDTLRRFHIEEENASGPMAQVVGHME
                     AIAADTGCSIVFLHHASKSAAMMGSGDQQQASRGSSVLVDNIRWQSYLSGMTQGEAEI
                     LGVDDCQRGYFVRFGVSKANYGAPFQELWFRRHDGGVLKPAVLERQCKVKRRQREEA"
     gene            complement(2287..2508)
                     /locus_tag="RH033_004506"
     CDS             complement(2287..2508)
                     /locus_tag="RH033_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HDV2993586.1"
                     /translation="MQNIKTLINRKKLLEMIPLSTRTIYNLEQRGDFPRRIALTSRNV
                     AWDLSEVEEWIEARKSSGDQAARPGPIEG"
     gene            complement(2656..3858)
                     /locus_tag="RH033_004507"
     CDS             complement(2656..3858)
                     /locus_tag="RH033_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDV2993587.1"
                     /translation="MLTDTKLRNLKPRDKLYKVNDREGLYVAVTPAGSISFRYNYSIN
                     GRQETITFGRYGVGGITLAEARELLGDAKKMVAAGKSPAKEKARDKARVKDAETFGAW
                     AEKWLRGYQMADSTRDMRRSVYERELKPKFSNQKLVEITHEDLRALADAIVERGAPAT
                     AVHVREIVLQVFRWAIERGQKVENPAELVRPTSIARFEPRDRALTPEEIGLMYQYMER
                     VGTSPTNRAAAKLLLLTMVRKSELTNATWSEINFSEALWTIPKERMKRRNPHLVFLSQ
                     QALDIFIAMKTFAGGSDFVLPSRYDSDAPMSAATLNQVLTLTYKAAQKDGKSLTKFGP
                     HDLRRTASTLLHEAGYNTDWIEKCLAHEQKGVRAVYNKAEYREQRAAMLQDWADMIDE
                     WTSGGSKG"
     gene            4167..4996
                     /locus_tag="RH033_004508"
                     /pseudo
     CDS             4167..4996
                     /locus_tag="RH033_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin-binding protein"
BASE COUNT         1208 a         1330 c         1283 g         1175 t
ORIGIN      
        1 tgtcgggcaa tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt
       61 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
      121 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
      181 tgatgcatgg ttactcacca ctgcgatccc cgggaaaaca gcattccagg tattagaaga
      241 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
      301 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc
      361 gcaatcacga atgaataacg gtttggttga tgctagtgat tttgatgacg agcgtaatgg
      421 ctggcctgtt gaacaagtct ggaaagaaat gcataagctt ttgccattct caccggattc
      481 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat
      541 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
      601 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
      661 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttctg
      721 atagttagtc tttgtagaga cacaaacctg aaattccgga caccgcgctt caggacatct
      781 ccctggacgc cgatatctgg cagtatccgg acggtactat cgaacgccgg gccaacggta
      841 ctaccctgcc ctttatgact gacgaccggc tatctcaccc acgcacttcg gccatgtcga
      901 tacgggcata tggacgatgc cccaggatcg cttcggcaat acgcggattc agggcaaccc
      961 atagcctgcc gtccgtctcg tcaaaagcat gactcatcag gtggaacgcg gcttgccgcc
     1021 gtcccttcgt cacaaggatg gtgacgttcg acatgcgcag caggctggct ttgagcgcct
     1081 tgatgttgtc gccgctatcc gtcatgcccg tttctgagag cagtttggtc aggctctcac
     1141 gaaccaccaa gccgtcttgc tcaatggctt cgaaacgggg ttcaaggaat agccgtagct
     1201 gtcgccccgt ctcactggtc ggttccgggg ttagcaagat gccgttcggg ccgccaaggg
     1261 ccacgatgcc ttgcaaaaga cgcatatcat cggccccgag aggttcgaat ccgacgaaac
     1321 gcatggattc gtcctcgcca aaggtgtagg tcacgtccag cttgcagcgt ttgcgatcgc
     1381 cacgcttgag gctgcggaac aggccaggtg ccaaacagtg cgcgggatca tgtcggacgt
     1441 gggtcaggtc atgcttaggc ttcttcacgc tgtctccttt tcaccttgca ctgccgctcc
     1501 agtacagcag gcttcaacac gccgccgtcg tgccgtctaa accagagttc ttgaaaaggt
     1561 gcgccatagt tggccttgct gacgccaaag cggacaaaat acccacgctg acaatcatcg
     1621 acccccagta tctcggcctc gccttgcgtc atgccggata ggtacgattg ccaacggatg
     1681 ttatcaacca gtacggacga tccacgactg gcctgctgtt gatcgcccga ccccatcatg
     1741 gctgcgcttt tactcgcgtg gtgcagaaac acaatggagc agcctgtatc agcggctatg
     1801 gcctccatgt gcccaacaac ctgcgccatc ggcccgctag cgttctcctc ctcaatgtgg
     1861 aagcgccgca aagtgtccaa gatcatcaag cgacgacctt cagcggctcg tttgagagca
     1921 tcgaaccagc tagcagccat gatgtttggg catttaccga tcaagggttc aatgagcaaa
     1981 ccatcagcca cggcttgccg ttccgctgcg ctgaggtgtg ccccaagggc gtgcagacga
     2041 tggtgaatag cggccggtgg atcttcagca ggcagataga ccacttgccc ggtgggaaac
     2101 tcgcctattt caagtaaatc aggcccgcct gcaatctgtg cagccaattg aagggccaac
     2161 atggatttac cagccccacc aggcgacaca agagcaccaa cagtgccagc aaccatatta
     2221 ggcagaacat aatcaatggg tggcggcaat tccgtgaaag ccgccataag atcaagagcc
     2281 atataactag ccctctatag ggccaggtcg cgcagcttga tcacccgatg atttacgtgc
     2341 ctcaatccac tcttcgacct ctgacaaatc ccaggcgaca tttctactgg ttaacgcgat
     2401 acggcgtgga aaatcccctc gctgctccag gttataaatt gttcgtgtcg aaagcgggat
     2461 catctccagg agcttcttcc tgttgatgag ggtctttata ttttgcatgg gtcaatacct
     2521 ctcaaatgaa tagttgctat tcatcgagta gctgcgcgaa gcagctaatc ggtaattgac
     2581 ctgcccgatg ccaatggcgg ccttatttat aaattcacgg catcaacttc aatgctatca
     2641 aagatagcaa aaacatcaac ccttactgcc tcccgaagtc cactcatcaa tcatatcggc
     2701 ccaatcctgc aacatcgccg cccgctgctc gcggtactca gccttgttgt agacagccct
     2761 cacgcccttc tgctcgtgcg ccaggcactt ctcgatccag tcggtgttgt agccggcctc
     2821 atgcaacagc gtgctggctg tgcgccgcaa atcatgcggt ccgaacttgg taagtgactt
     2881 cccatctttc tgcgccgcct tgtaagtcag cgtcagcacc tggttaagtg tggcagcgct
     2941 catcggcgca tccgagtcgt accgtgatgg caaaacgaag tcggaaccac cggcaaaggt
     3001 tttcatggca atgaaaatat ccagagcctg ctgggacaga aataccaggt gcgggttacg
     3061 gcgtttcatc cgctcctttg gaatcgtcca caacgcttcg ctgaaattga tctcgctcca
     3121 ggtcgcattg gtcagctcgc tcttgcgcac catcgtcagc aataacagct tggccgccgc
     3181 acggtttgtt gggcttgtgc ccactcgctc catgtactgg tacatcagcc caatttcttc
     3241 tggcgtcaac gctcggtcac gtggctcgaa tcgagcgatg cttgttgggc gcaccagttc
     3301 tgccgggttt tcgaccttct gcccacgctc gatggcccag cgaaatacct gcaacacgat
     3361 ttcacgcaca tgaacggcgg tggccggtgc gcctcgctca acaatggcat cagccagcgc
     3421 ccgcaagtct tcgtgggtga tctccaccag cttctgattg ctgaatttcg gcttcagctc
     3481 acgctcataa accgagcggc gcatatcgcg ggtggagtcg gccatctggt agccacgcag
     3541 ccacttctcc gcccaggcac cgaacgtctc tgcatctttc acccgcgcct tgtctcgggc
     3601 tttctccttg gccggcgact tgcccgccgc aaccatcttc ttggcgtcac ccaacagctc
     3661 gcgggcttcg gccagggtga tgccaccgac accataacgc ccaaaggtga tggtctcctg
     3721 ccgaccgttg attgagtagt tgtaacggaa cgagatggag ccggctggag tcactgccac
     3781 atagagacct tcccggtcat tcactttgta gagtttgtcc ctgggcttga gattgcgcag
     3841 cttggtatcg gtcagcatgt accttgtcct tcttcgtctc cgataccatg ctgaaaaaat
     3901 ctcgaattta tcgtattttt tcttacgaaa cagtaactta tagaatatta ataccatgaa
     3961 cacacaaaag aacgcagcat ggtatcggca tcaatcatgg catcgccaaa ccataatacc
     4021 agctttaatg ccatgctcaa acggtgcttg gcgcttcctc ccgttgccag atcgtgccaa
     4081 acacaaacaa aaaaaagccc gcaattacgc gggcttagcg gcatttcatg ccatcaggtg
     4141 cctggctgtg ccagacgcgg aatcattccc gccggattat aaatgtgtct gatggtatca
     4201 ataacacaga tgcagcgacg gtagggcaac tgaatgaacg atttgatgat gcacaggtct
     4261 ttttgttgca gacgaatgaa agaattgacg agacagataa acgtctttca acagttcatg
     4321 ctgaactcag cagggatatt attgcaggca catcggcagc ggtgacgtat acagatgtaa
     4381 cagcgcttgc acttcaggat gagataaagg atgggacgaa caaagtacgt gatgagttga
     4441 aatcacaggg agacagtctg cgtggtgaga taggcggtgt atacagagat gcccgtgctc
     4501 atactgacag tcaggtcaca gcagtacgtg atgaactgaa ggcagaggga gacagtctgc
     4561 gcggtgagat aggcggtgta tacagagatg cccgtgctca tactgacagt caggttacag
     4621 cagtacgtga tgaactcagc agggatatta ttgcaggcac atcggcagcg gtggcgtata
     4681 cggatgcatc ttctcttgct cttcaggatg aaataaaaga aaaagcggat gaaacagttc
     4741 aggtttcccg ggcttataca gataaaagtg tacgggatgc ccgtaaagaa gcgaaaagcc
     4801 aggcagaaca tctcagcgat gtgcttgtga agaacagggc gcagacggat gcagcaatag
     4861 cctcaaatac agcagcgata aggaataaca gtcatcgtct ggatttgacg gaagcctggc
     4921 agaagatggc gacagagaga atgaataata tgcaggagca gattaaagag aaccggaagg
     4981 agttaaggga gagtgc
//
LOCUS       DAPHCV010000202         4981 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.204, whole genome shotgun sequence.
ACCESSION   DAPHCV010000202 DAPHCV010000000
VERSION     DAPHCV010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.204"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..235
                     /locus_tag="RH033_004509"
     CDS             <1..235
                     /locus_tag="RH033_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993588.1"
                     /translation="CVVLNPEKSHPYIDDMAMFLSPLPVVLEWHCVMCGQHFSGKKFC
                     EACGTGIYCRAACTKYVYRYQPDLFHYCGGAIT"
     gene            990..3047
                     /locus_tag="RH033_004510"
     CDS             990..3047
                     /locus_tag="RH033_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDV2993589.1"
                     /translation="MDLVDSVEAGKLTIRELIDALVKDKNYTASKWEQRYREFTTLLQ
                     QTSTFAEPETDDLVKRLWYERDNGIASIRQGVPSLAEYQQSLPLLRELTERVRQQPNE
                     ATYQYVGSALQQAKEIGQLKRMYWSLRNRVFAAFSPENYTSTVDENAFNKAAEFLNQR
                     FHLGLVLTGNWLQKNYELKQAIHAQSPDTDPYYVNMAIWHIYELLRERDNEQKQEKVA
                     SSEPIENKTIPHSPTNVIFFGPPGTGKTFTLQQKMKEYTSHAVPADREAWLDSRLESL
                     NWMQVITLVLLDLGKLAKVRQIIEHMWFRRKALLNGRNGNLSNTAWEALQAYTIPESL
                     TVDYKNRREPAVFDKTDNSEWFLVDSQLEQVEDLLALHAELKRGPKSAEAIQRFAVVT
                     FHQSYGYEEFIEGMRARSDESGNISYPIEPGVFMRLCQRANADPAHRYAIFIDEINRG
                     NISKIFGELISLIEVDKRAGMPNAMSLQLSYSGDHFSVPANVDIIGAMNTADRSLALL
                     DTALRRRFDFVEMMPDLSLLSGAKVKGIELESLLEKLNSRIEALYDREHTLGHAFFMP
                     VKNALDTGDEETAFKQLKIAFQKKIIPLLQEYFFDDWNKIRLVLADNQKQDDNQQFVI
                     EKTDDLDTLFGNNHGLRRHNQQSTTYELKDFDQGVWNMPKAYRSIYQPQRTTPDEQVV
                     NHE"
     gene            3040..4326
                     /locus_tag="RH033_004511"
     CDS             3040..4326
                     /locus_tag="RH033_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HDV2993590.1"
                     /translation="MSEVISIFEYDLLGSGKAASVGAKLLPQQVFDYLETLSLTSEQG
                     SQFLKLTSRSGFKLLQVQNYAGMLSTPHGLQLEILPKIGKNLTLASARETFITMLSHL
                     PRFRHIQTQQATIQAQRMPLLEIFISQFLQSVSQLLKQGLRSDYVSEKGNLAFMKGKL
                     MLSAQLRHNAVNRHKFCVDYDEYMPDCAANRLLHSTLDKLLSLKLSSENQRWLYELRF
                     AFDGIPLSRDIESDLNSLRIERGMTHYSEPIAWAQLILRGMSPSALQGNTKAISLLFP
                     MEAVFESFVAQTLPYELPSHLKVFSQAATYSLVKHGLKDCFKLRPDLLIQSRQPIQTK
                     MVMDTKWKQVNSSQQKKSLYGLAQSDFYQMFAYGQKYLGGTGEMYLIYPAHDDFSQPI
                     PQHFAFSETLKLWVVPYRIMAKRGERMMWASDVLAT"
     gene            4524..4733
                     /locus_tag="RH033_004512"
     CDS             4524..4733
                     /locus_tag="RH033_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HDV2993591.1"
                     /translation="MNKQQIAAKIWESANQMRSKIEANEYKDYILGFIFYKYLSDQFV
                     RFVTRQGDTRSWIVVHRTVKTYLKS"
BASE COUNT         1428 a         1104 c         1143 g         1306 t
ORIGIN      
        1 ctgcgtggtg ctgaatccgg aaaaatccca tccttacatt gatgatatgg cgatgttttt
       61 atcgccatta cctgtcgtgc tggagtggca ttgtgtgatg tgcgggcagc atttttccgg
      121 gaaaaagttc tgtgaagcct gtggcacggg gatttattgc agagcggcat gtacgaagta
      181 tgtttacaga taccagccag atttgttcca ctactgcgga ggtgcgatca cttaatccaa
      241 aatccccgct ctgctcaggg cgttcaggct gactatcaat ggcatacatt ttatccggtg
      301 tttcgcacct acaccgtgca acggcgcgac accggatatc cgcagtcctg cttacctgaa
      361 taaaacccgg ggtattgatt gtgagcggtg ggacgaaatc gcacggaggt gtcaatcaac
      421 cacacgcgtg tgtcgtctcc gtatttatca gtgtctcagg acggttaatt ccgagggcaa
      481 catttatgga ggcccagtgc gcgctgatga ccgtcacttc tgacctgctc ccgatatgcc
      541 ggaatataac ggtcagagag gcatatttcg atgcccctat tctggagcag ttggttatgg
      601 atgccatagg ctatgtaagc gctttgtgtc accggacgaa acggcgcagt accagaagca
      661 cccaggatgt ggaacaggtt tattggttac ctgacagcga agatagactc aaagagcagt
      721 cctgaacgtt cagtgatttt tcctgcgttc gtcgtggtgt acgcaatcag gaatcccgtt
      781 atctcattgt gaaatgcaag aagtgcgact gaaggtgaat gcgcccaaaa tccgttcgag
      841 agtgcaaaat catttttatg aaaaacatgc attatacatc gaaaatctta cttatggcgc
      901 ttggctacaa ttgcatgctt ggtaatatag attataaaac accagactag ctgcctagta
      961 agtaatgtaa tagcagagga aatatcacga tggatcttgt agatagtgtc gaagctggta
     1021 agctgacgat cagagaattg attgatgccc tggtgaaaga taaaaattac acagcttcca
     1081 agtgggagca gcgataccgg gaatttacga ccttgctaca acaaacctca acttttgctg
     1141 agcctgaaac ggatgattta gtcaagaggc tctggtatga acgtgataac ggtattgcca
     1201 gtattcgtca gggcgttcca tcattagcag aatatcagca aagcctccca ttgcttagag
     1261 aactcaccga acgtgttcgt caacaaccaa atgaagctac ctaccaatac gtaggcagtg
     1321 cactgcagca ggccaaagag atcggacagc ttaaacgcat gtattggagt ttaagaaatc
     1381 gtgtctttgc cgcgttctcg ccagaaaact acaccagtac tgtggatgag aatgctttca
     1441 ataaagcagc agaattccta aatcaacgct tccatctcgg tttggtactg actggaaatt
     1501 ggttacagaa aaactatgaa ttgaaacaag ccatacacgc ccaatctcct gacacagatc
     1561 cctattacgt gaatatggct atctggcata tctatgaatt gctccgtgag cgcgataatg
     1621 aacagaagca agagaaagta gcaagcagtg agcccatcga gaacaagact attccacatt
     1681 caccaactaa cgtgatcttc tttggccccc caggcactgg caagaccttc acgttgcagc
     1741 aaaaaatgaa agagtatact tctcatgcag ttcctgctga tcgtgaagcc tggctggatt
     1801 ctcgtcttga atcgttgaac tggatgcagg ttataacgct ggtgctgctc gatcttggga
     1861 aactagccaa agttcgccaa attattgaac atatgtggtt tcgacgtaag gcattattaa
     1921 acggtcgtaa tggcaatcta tcgaatactg cctgggaggc tttgcaagcc tatacaattc
     1981 ccgaatcgtt aactgttgat tataaaaatc ggcgtgaacc tgccgtattt gacaaaacag
     2041 ataacagcga atggtttcta gttgattcac agctcgagca agtggaggat ttgttagcgc
     2101 tccacgccga acttaaacgt ggtccaaaat ctgccgaagc catccagcgt tttgcggtgg
     2161 ttacgttcca ccaatcttac ggctatgaag aatttattga aggtatgcgt gcacgctctg
     2221 acgagagtgg caatatatct tatcccattg agccgggtgt ttttatgcgc ctttgccaac
     2281 gtgctaatgc cgatccagca catcgctacg ccattttcat tgatgagatc aatcgcggaa
     2341 acatatccaa gatctttggc gaactaatct cactcattga agtagacaag cgtgctggta
     2401 tgcccaatgc aatgagtctg caactgtctt atagcggtga tcactttagc gtgcccgcca
     2461 atgtcgacat catcggagcc atgaatacag cagaccgttc tttagctctg ctggacacgg
     2521 ctttgcgccg tcgctttgac tttgtcgaaa tgatgccaga tctttcttta ctgagtggag
     2581 ctaaggtgaa aggcatagag cttgagtcgt tgttagaaaa actcaatagc cgcatcgaag
     2641 ctctttacga tcgtgagcat acactaggac atgcattctt tatgccggta aaaaatgctc
     2701 tcgataccgg tgatgaagaa actgcgttta aacaattgaa gatcgcattt cagaaaaaga
     2761 tcattccgct tttacaggaa tactttttcg atgactggaa caagatccgg ttggtgctgg
     2821 cagacaatca aaagcaagac gacaaccagc aattcgtgat tgagaaaact gacgatctcg
     2881 atacgctttt tggtaacaac catggtttac gacgccataa tcagcaatca acaacttacg
     2941 agctcaaaga tttcgatcaa ggggtctgga atatgccaaa ggcttatcgt tcaatttatc
     3001 agccacagcg gaccactcct gatgagcagg tcgtaaacca tgagtgaagt tatctcaatt
     3061 tttgaatatg acctgttggg gagtggaaaa gcggcttcag ttggcgcaaa gttgttgcct
     3121 caacaggtct ttgattattt agaaacactt agcctaacaa gtgagcaagg aagccagttt
     3181 cttaaactca cctctcgttc aggtttcaaa ttgctgcagg tgcaaaacta cgcggggatg
     3241 ctttctacac ctcacggttt gcagcttgag atactgccca agattggtaa aaacctcacg
     3301 cttgcgagtg cgcgagaaac attcattacg atgctaagtc acctgcctag gtttcgacac
     3361 attcaaaccc agcaggccac tattcaggcg caacgcatgc ctttactcga gatttttatc
     3421 agccaatttt tgcaaagcgt cagccaattg cttaaacaag gtttacgctc cgactatgtg
     3481 agcgagaaag gtaatctggc ttttatgaag ggcaaactga tgctctctgc acaactgcga
     3541 cataacgcgg taaatcgcca taagttttgt gtcgattatg atgagtatat gcctgattgt
     3601 gctgccaatc ggctcttaca ctccacactg gataagttac ttagtctgaa gctgtcatca
     3661 gagaatcaac gctggcttta cgaactgcgc tttgcttttg acggtattcc acttagtagg
     3721 gatatagaga gcgatctgaa cagcttacgc attgagcgtg gtatgactca ttacagcgag
     3781 ccaatagctt gggcgcagtt gatcctgaga ggaatgagcc caagtgcatt gcaaggaaac
     3841 accaaagcga tatcactttt gtttcctatg gaagcggtat ttgaatcctt tgtggcacag
     3901 accctaccct acgaattacc ttctcaccta aaagtttttt ctcaagcagc aacgtattct
     3961 ttggtaaagc atggacttaa agattgcttt aagcttcgcc cagacttgct gattcaatct
     4021 cggcaaccga ttcaaaccaa aatggtgatg gatacaaaat ggaagcaggt gaatagcagc
     4081 cagcaaaaaa aatcacttta tgggctagcg caatccgact tctatcaaat gtttgcctac
     4141 ggccaaaaat accttggcgg aacaggcgaa atgtacctga tttaccctgc gcatgatgac
     4201 tttagccaac cgataccgca gcactttgct ttttcagaga ctttaaaatt atgggttgtg
     4261 ccataccgga taatggcaaa acgtggtgag aggatgatgt gggcaagtga tgtgttagct
     4321 acatagtgac taagccattt ggaatgggcc agtccaaaac atcgacgctg tctcaggcaa
     4381 agtggttgtg ttgacaggaa tggagacagt agtccctcat taaccctgac tcgaagccgc
     4441 tgccgctagc aagacgctct tatcaaaagt acaatcaaaa ccctaaaaca gcccttttag
     4501 aattacgttt aacaggattt atagtgaata aacaacagat tgctgcaaaa atctgggaat
     4561 ctgccaacca gatgcggtct aaaattgaag ctaacgaata caaagattac atactcggtt
     4621 ttatcttcta caagtaccta agcgaccagt ttgtgcggtt tgtcaccaga caaggtgaca
     4681 cccgaagctg gatcgtcgtt catcggactg tgaagacata cttaaagagc tgatatgggc
     4741 gttcagtgat ttcgtccgcg atccggaagc cctttatctc attggaatac ttgctatacc
     4801 acactttcct tgcagtcatc atcgaaaagc cacctattgg tagtgttact gtggtttttg
     4861 aggtaaaaat agtgacagca aaatctgagc agcataacaa gagacgtaaa gaatggaact
     4921 gttaggataa cttgactaac aatacttcgg cacaagaatt acaatcatta gaaatctact
     4981 t
//
LOCUS       DAPHCV010000203         4957 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.205, whole genome shotgun sequence.
ACCESSION   DAPHCV010000203 DAPHCV010000000
VERSION     DAPHCV010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4957
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.205"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            96..1247
                     /gene="yiaY"
                     /locus_tag="RH033_004513"
     CDS             96..1247
                     /gene="yiaY"
                     /locus_tag="RH033_004513"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HDV2993592.1"
                     /translation="MAASTFFIPSVNVIGADSLNDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     NIPAGLRDLNVKEEDIPVLATNALKDACGFTNPIQATHEEIMAIYRAAM"
     gene            1448..2986
                     /gene="aldB"
                     /locus_tag="RH033_004514"
     CDS             1448..2986
                     /gene="aldB"
                     /locus_tag="RH033_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HDV2993593.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTIEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3205..3348)
                     /locus_tag="RH033_004515"
     CDS             complement(3205..3348)
                     /locus_tag="RH033_004515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993594.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGTPMVIYGLVRPLTIE
                     TRD"
     gene            3531..3854
                     /locus_tag="RH033_004516"
     CDS             3531..3854
                     /locus_tag="RH033_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HDV2993595.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDVPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            3860..>4957
                     /locus_tag="RH033_004517"
     CDS             3860..>4957
                     /locus_tag="RH033_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDV2993596.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSAAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLR"
BASE COUNT         1244 a         1171 c         1304 g         1238 t
ORIGIN      
        1 taaaaatatt ttaataaacg gtaatgttga tcatattttc aaatgaaaat cctcagtaag
       61 ctgcccgccc ttttttacac tttcaggagt gtgttatggc agcttcaacc ttttttattc
      121 cttccgtgaa tgttattggt gctgattcat tgaatgatgc aatgaatatg atggcagatt
      181 atggatttac ccgtacctta attgtcaccg acaacatgtt aacgaaattg ggtatggcgg
      241 gtgatgtgca aaaagcactg gaagaacgca atatttttag cgttatttat gatggcaccc
      301 aacctaaccc caccacggaa aacgtcgccg caggtttgaa attacttaaa gaaaataatt
      361 gcgatagcgt gatttcctta ggcggtggtt ctccacacga ctgcgcaaaa ggtattgcgc
      421 tggttgcagc caacggcggt gacatccgcg attatgaagg cgttgaccgc tctgcaaaac
      481 cgcagctgcc gatgatcgcc atcaacacca cggcgggtac ggcctctgaa atgacccgtt
      541 tctgcatcat cactgacgaa gcgcgtcaca tcaaaatggc gattgtggat aaacatgtaa
      601 ctccgctgct ttctgtcaat gactcctctc tgatgatcgg tatgccgaag tcactgaccg
      661 ctgcaacggg tatggatgcc ttaacgcacg ctatcgaagc gtatgtttct attgccgcca
      721 cgccgatcac tgacgcttgt gcactgaaag ccgtgaccat gattgccgaa aacctgccgt
      781 tagccgttga agatggcagt aatgcgaaag cgcgtgaagc aatggcttat gcccagttcc
      841 tcgccggtat ggcgttcaat aatgcttctc tgggttatgt tcatgcgatg gcgcaccagc
      901 tgggcggttt ctacaacctg ccacacggtg tatgtaacgc cgttttgctg ccgcatgttc
      961 aggtattcaa cagcaaagtc gccgctgcac gtctgcgtga ttgtgccgct gcaatgggcg
     1021 tgaacgtgac aggcaaaaat gacgcggaag gcgcagaagc gtgcatcaat gccatccgtg
     1081 aactggcgaa gaaagtgaat atcccggcag gcctacgcga cctgaacgtg aaagaagaag
     1141 atattccagt gctggcaacc aatgccctga aagatgcgtg tgggtttact aacccgattc
     1201 aggcaactca cgaagaaatt atggcgattt atcgcgcagc gatgtaatca tcattcccac
     1261 aatggctgtc taattgtcag ccatttttta aactatctca aaatttgcga tttttgagag
     1321 agttttagtt atctctgtaa tccttgcccg taaattcgtg atagccgtcg taaagctgtt
     1381 accgactggc gaagatttcg ccagtcacgt ctacccttgt tatacctcac accgcaagga
     1441 gacgatcatg accaataatc ccccttcagc acagattaag cccggcgagt atggtttccc
     1501 cctcaaatta aaagcccgct acgacaactt tatcggtggc gaatgggtag cgcctgccga
     1561 tggcgagtat taccagaacc tgacgccggt gaccgggcag ctactgtgcg aagtggcgtc
     1621 ttcgggcaaa cgagacatcg atctggcgct ggatgctgcg cataaagtga aagataaatg
     1681 ggcgcacacc tcggtgcagg atcgcgcggc gattctgttt aagattgccg atcgaatgga
     1741 acaaaacctt gaacttttag cgacggcaga aacctgggat aacggcaaac ccattcgcga
     1801 aaccagtgct gccgacgtgc cgctggcgat tgatcatttt cgctatttcg cctcgtgtat
     1861 tcgggcgcag gaaggtggga tcagtgaagt ggatagcgaa accgtggcct atcatttcca
     1921 tgaaccgtta ggcgtggtgg ggcagattat cccgtggaac ttcccgctgc tgatggcgag
     1981 ctggaaaatg gctcccgcgc tggcggctgg caactgtgtg gtgctgaaac ccgcacgtct
     2041 tactccgctt tctgtactgc tgctaatgga aatcgtcggt gatttactgc cgccaggggt
     2101 ggtgaacgtg gtcaacggcg caggtgggga aattggcgaa tatctggcga cctcgaaacg
     2161 catcgccaaa gtggcgttta ccggctcaac ggaagtgggc caacaaatta tgcaatacgc
     2221 cacgcaaaac attattccgg tgacgctgga gctgggcggc aaatcgccaa atatcttctt
     2281 tgctgatgtg atggatgaag aagatgcctt tttcgataaa gcgctggaag gctttgcact
     2341 gtttgccttt aaccagggcg aagtttgcac ctgtccaagc cgtgctttag tgcaggagtc
     2401 tatctacgaa cgctttatgg aacgcgccat ccgccgtgtc gaaagcattc gcagcggtaa
     2461 cccgctcgac agcgtgacgc aaatgggcgc gcaggtttct cacgggcaac tggaaaccat
     2521 cctcaactac attgatatcg gtaaaaaaga gggcgctgat gtgctcaccg gcgggcggcg
     2581 caagctgctg gaaggcgaac tgaaagacgg ctactacctc gaaccgacga ttttgtttgg
     2641 tcagaacaat atgcgcgtgt tccaggagga gatttttggc ccggtgctgg cggtaaccac
     2701 cttcaaaacg atagaagaag cgctggagct ggcgaacgat acgcaatatg gcctgggcgc
     2761 gggcgtctgg agccgcaacg gtaatcttgc ctataagatg gggcgcggca tacaggcagg
     2821 gcgcgtgtgg accaattgtt atcacgctta cccggcacat gcggcgtttg gtggctacaa
     2881 acagtcgggc atcggacgcg aaacccacaa gatgatgctt gagcattacc agcaaaccaa
     2941 gtgcctgctg gtgagctact cggataaacc gttggggctg ttctgatata agaagctggt
     3001 cgcattgggt attcattgcc tgatgcgacg cttacgcgtc ttatcatgcc tacggggacc
     3061 tgaccgtagg ccggataagg cgtacacgcc gcatccggca taaacaaagc gcacgttgtt
     3121 acaatttacg ttttaaacct acgtgcgcaa taacccagac tacgtcaaca gaagtgcgtt
     3181 tctggggcgt gttttgccag gagtttaatc tcgcgtttcg atggtgagcg ggcgaaccag
     3241 gccataaatc accattggcg ttccgaggcc aatcagcaat aaacggtaga taattgaagg
     3301 tttaatacgc ttaatctgta ctgtggatgt tttactggag ttattcatta gtttggccaa
     3361 tagttccatt atttcaatat gataagcctc gcactgttga aaagccaggc tgcacacttc
     3421 agaaagtata taaaaaacaa tatgtcgtta tataaataag gaaattgcac cgcattatgg
     3481 tatttcataa tatgcgttaa ttgttctttt ctatattctc tgagggaaat atgttcctgg
     3541 attattttgc attgggagtg cttatttttg tatttctggt gatattctat gggattatta
     3601 ttttacatga tgttccctac ctgattgcca aaaaacgtaa tcatcctcat gccgacgcca
     3661 ttcatgttgc tggttgggtg agtcttttta cgttacatgt tatctggcca tttttgtgga
     3721 tttgggccac gctttaccgc ccggagcgtg gatggggaat gcaaaaccat gactcctccg
     3781 ttgtgcaact acaacagcgt attgccgggc tggagaaaca gctcgctgac atcaaatcct
     3841 cttctgccga gtaatactta tggatttact gattatttta acctatgtgg ctttcgcatg
     3901 ggcaatgttt aagatcttca aaattcctgt aaataaatgg accattccca cagcggccct
     3961 gggaggcata tttattgtca gcggtctaat tctgttaatg aactataacc atccgtatac
     4021 ctttaaagcg caaaaagcgg ttatttctat tcctgttgtc ccacaggtga caggcgtggt
     4081 gatcgaagtg acggacaaga aaaatacgct gattaaaaaa ggtgaggtgc tatttcgact
     4141 ggacccgacg cgttatcagg ctcgggtgga tcggctgatg gcggatatcg ttaccgcaga
     4201 acataaacag cgggcgttgg gcgcagagtt agatgagatg gcggcgaata ctcagcaggc
     4261 aaaggccacg cgggataagt tcgctaaaga gtatcagcgt tacgcacgtg gcagccaggc
     4321 gaaagtaaac ccgttttcag aacgcgatat cgatgtggcg cggcaaaatt atctggcgca
     4381 ggaagcctcc gtaaaatcag cggcggcgga acaaaaacag atccagagcc agctggatag
     4441 cctggtgttg ggtgaacatt ctcaaatcgc cagcctgaaa gcgcagctcg cggaagcaaa
     4501 atataacctt gagcagacga tagtgcgtgc gccgagcgat ggttatgtga cccaggtgct
     4561 gattcgtccg ggtacctatg ccgcgtcgct gccgctacgt ccggtgatgg tgtttatacc
     4621 cgatcagaaa cgacaaatcg tggcgcagtt ccgtcagaac tccttgctgc gcctggctcc
     4681 cggcgatgat gcggaagtgg tatttaacgc tctgccaggc aaagtgttca gcggtaagct
     4741 ggcagccatt agtccagctg ttcccggtgg agcttatcag tcgaccggca ccttacagac
     4801 gttaaacaca gcgccgggtt cagatggcgt tatcgcgacc attgaactgg atgagcatac
     4861 tgatttgagc gcattaccag acggtattta cgcccaggtg gcggtctatt ctgatcattt
     4921 cagccatgtc tcggtgatgc gcaaagtact gttacgc
//
LOCUS       DAPHCV010000204         4944 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.206, whole genome shotgun sequence.
ACCESSION   DAPHCV010000204 DAPHCV010000000
VERSION     DAPHCV010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.206"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(178..1371)
                     /locus_tag="RH033_004518"
     CDS             complement(178..1371)
                     /locus_tag="RH033_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV2993597.1"
                     /translation="MTKATVNTGTFVSQNGTLIVDASSENTLDISGKASGDLRVYSAG
                     SLDLINEQTAFISTGKDSTLKATGTTEGGLYQYDLTQGADGNFYFVKNTHKASNASSV
                     IQAMAAAPANVANLQADTLSARQDAVRLSENDKGGVWIQYFGGKQKHTTAGNASYDLD
                     VNGVMLGGDTRFITEDGSWLAGVAMSSAKGDMTTMQSKGDTEGYSFHAYLSRQYNNGI
                     FIDTAAQFGHYSNTADVRLMNGGGTIKADFNTNGFGAMVKGGYTWKDGNGLFIQPYAK
                     LSALTLEGVDYQLNGVDVHSDSYNSVLGEAGTRVGYDFAVGNATVKPYLNLAALNEFS
                     DGNKVRLGDESVNASIDGAAFRVGAGVQADITKNMGAYASLDYTKGDDIENPLQGVVG
                     INVTW"
     gene            complement(1393..2469)
                     /locus_tag="RH033_004519"
                     /pseudo
     CDS             complement(1393..2469)
                     /locus_tag="RH033_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308377.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(2511..3272)
                     /gene="hyxA"
                     /locus_tag="RH033_004520"
     CDS             complement(2511..3272)
                     /gene="hyxA"
                     /locus_tag="RH033_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HDV2993598.1"
                     /translation="MRFVLFCPSGIVPAQFAALSTGSVGNVTCIRTEEELRNKLRHRP
                     QNVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VERVISIANASGGFSGLKHAEILPVMDSYSVFMKEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNNFHGDGNA
                     LETTNAMTLRENGNTNRYSVVVNAGTQALSLHKYK"
     gene            complement(3426..4220)
                     /gene="hyxR"
                     /locus_tag="RH033_004521"
     CDS             complement(3426..4220)
                     /gene="hyxR"
                     /locus_tag="RH033_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
                     /protein_id="HDV2993599.1"
                     /translation="MKKPLVIISACQFTRLALESLIPTDRYIVRVYSNVTTEVEFVLK
                     TSCGYLLADIPSLPSGELVLLACLVRLRSDAGQWQVCLTGDSDWFDNAPAASLYNGFP
                     RIGTMLTASRYQNQIIRWLLRPLPGDMSDFWLLTARELSVLKILMQGMSFSEIARYEQ
                     RSSKTLHAIATRALMKLGLGTLSDFRLLYTGCGDSRVKRNIRLHAKYIGQHSAHRLTQ
                     YVQNMVSGILYPEKVAVGESINSDAISGKVLSGQRKNMSKKRPGLL"
     gene            complement(4279..4842)
                     /gene="ipbA"
                     /locus_tag="RH033_004522"
                     /gene_synonym="fimX"
     CDS             complement(4279..4842)
                     /gene="ipbA"
                     /locus_tag="RH033_004522"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060710320.1"
                     /note="IpbA is also known as HbiF and FimX; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
                     /protein_id="HDV2993600.1"
                     /translation="MTRKYLTQDEVYRLMDAAQSMSFPERNRCLIMMAFIHGFRASEL
                     LDLRLSDIDASGKQLNIRRIKNGFSTTHPLLPDEYNLIKLWLKQRKLIENGVEGDWLF
                     LSRKRRPISRQHFFSIIREAGRRAGLAVKAHPHMLRHACGFALADNGVDTRLLQDYLG
                     HRNIQHTVRYTASNAARFKGVWKKKPR"
BASE COUNT         1301 a         1218 c         1061 g         1364 t
ORIGIN      
        1 tattcagaga ataaaatctg ccgcagataa atgaattaat agtctgtatc aggaatgttc
       61 gggttaaata tcagcaaata tccagcatca tgaatactgg atatttatag cctgcaggca
      121 gcatactgct gcctgcaggg ggtataacag tcgacagggg aaccgactgc tttattctta
      181 ccaggtcaca ttgataccaa ctacaccctg tagcggattc tcaatgtcgt cacctttggt
      241 gtagtcaagg cttgcatatg ctcccatgtt tttggtgata tcagcctgta cacctgcacc
      301 cacgcggaat gctgcaccgt caatgctggc attgacagac tcatcaccca gacggacttt
      361 gttgccatca gagaattcgt tcagtgcggc cagattcaga taaggtttaa cggtcgcgtt
      421 gcccacagcg aagtcataac ccacgcgcgt accggcctca cccagcacag agttatagct
      481 gtcagaatga acgtccacgc cgttgagttg ataatccaca ccttccagag tcagagcaga
      541 cagtttggca tatggctgaa taaacaggcc attaccgtct ttccatgtgt aaccgccttt
      601 aaccatcgca ccaaaaccat tggtgttaaa gtcagctttg atggtaccgc caccattcat
      661 caggcgaaca tctgccgtgt tgctgtagtg accaaactgt gcagcagtat caatgaagat
      721 accgttgtta tactggcggc tcaggtaagc gtggaagctg taaccttcag tgtcaccttt
      781 gctctgcatg gtagtcatgt cacctttcgc agaagacatc gccacaccgg ccagccagct
      841 accatcttca gttatgaagc gggtatcacc acccagcatt acaccattta catccaggtc
      901 ataggatgca tttcccgcgg tggtatgttt ctgtttaccg ccaaagtact gaatccatac
      961 gccgcccttg tcattttcgc tcagacggac agcatcctga cgggcggaga gcgtgtcagc
     1021 ctgcagatta gcaacgttag ccggagcagc tgccattgcc tgaatcacgg agctggcgtt
     1081 ggatgcttta tgcgtgtttt ttacgaaata aaagttacca tcagctccct gtgtcaggtc
     1141 atattgatac agaccacctt ccgttgtgcc tgtggctttt agagtgctgt ctttgccggt
     1201 agaaataaat gccgtttgtt cattgataag atccaatgaa cccgcactgt aaacacgcaa
     1261 gtcaccgctc gcttttccgc tgatatccag agtgttttca gaagaggcat caacaatcag
     1321 agtgccgttt tgagaaacaa acgtgccagt attgactgta gccttcgtga tatttaccgt
     1381 actgtctttt actgccagag catcaacagt tgaggaaccc gtaacattcc agacagaccc
     1441 gttttgcaga gatacgttgg catctcctgc ccctgaaact ttgcctttcc agactgaact
     1501 atcggaaaga tttatatcca gattactacc cgctttatcg ccgctattga aggtataagc
     1561 attaccttcc cagtaagagc gggcaacgtt catgtttaca tttgttgtgg taccgactga
     1621 attctcgctt tcagaaaggg ccaggatatc agagtagatg gttgaatcag caacagacaa
     1681 atttaaattt tgcgttgctg aacctgcgcc ttgtgccgaa gcagaggcat aaattgtttg
     1741 tttattatta gccgccccaa ttactgagcc atccttaata ttcaggttga ctgtatgagt
     1801 tcccgtatta ttcccggcaa taactattgc ttcaatccga ccattaataa tatttgagcc
     1861 agcaagggta atgttgtcgg ttgcatcacc gttaaaacta ttcaataagc tacccttttg
     1921 agcaaaaatt gccgtggagg cgttgctgga actcttatta ccttgagcat ttatggttgt
     1981 gccattaagc gtgacattat ttccctgggc cccttccaga gatatccctg tcgcactacc
     2041 tttttcattt tttgcaacga aattaccgcc attaacaaca acatcgccag ttgaatcaat
     2101 gaccattgca gtccgggcat aggtgccttc accttgagct gtgatggtgg agcctgtagc
     2161 attaatcaca acatttttgt caccgctaac ttttacacct ttaacgaatt ctttatctgc
     2221 cgttgattca gcagtaatat catgaccacc aaaagtaaca ttaaggtctt tatcttccgc
     2281 attaattgtt gcggctgtaa tctgtgtagt accactaata acaacatctt gttccagagt
     2341 tgtcttatct ttatccaatg taaccggtgt agttgatacc gcgttatcag cagcgaatgc
     2401 tggtgcagaa agagccataa caatagctgt tgctaatgcg ctgcggtaaa atgccttact
     2461 gtttttcatg aagaaattct ccattctggt aactaatttc aatttcccac ttatttatat
     2521 ttatgcagag ataaagcctg cgtccctgca tttaccacca cggaatacct gttggtattt
     2581 ccgttttcac gcaatgtcat ggcattcgtt gtttccaatg catttccatc accgtgaaaa
     2641 ttattgctgc tcagcagcag ggcttcccgc aatgacatgc tgtccggtat tccccaacgc
     2701 ttttttagcg actgctcggc aagccatatc tgtcttatcc ccgttttgag aaactgcgca
     2761 ctgtattccc agctgtgtcc ggcaagaagc aacgacaaca ccttcttttg cttttccggg
     2821 aagcgttctg acatgacgat tgtctttgtg cggttattca cctctttcat aaagacagag
     2881 taactgtcca taactggcaa aatttctgca tgtttcagcc ctgaaaaccc accagaggca
     2941 tttgcaatac tgatgacacg ctctactgac attcctggtt tcagtaacct caggtttttg
     3001 agaacgccag aaatactcac atctggtggc agaaaaaagg gtgcgacgca caacaccacc
     3061 acgaatgagt gatcccgata aaatcggaac cacatttcag cgcattcggc aggtcgccct
     3121 gccgatatca cgacattctg tggcctgtgc cgcaatttat tgcgtaactc ctcttctgtt
     3181 ctgatgcagg taacattgcc gacagaaccg gttgatagag ctgcgaactg cgctggtaca
     3241 attcctgagg gacagaaaag aacgaaacgc atccttccct cacagatgtc agtacgccag
     3301 cacgctgtac aggctgtaca gcgtgcctcc caatcctgca gcccagagca gtagaaatat
     3361 tttggctcct acactgtagc gagtccgtat gcgccgcagt ggggggtagt aacaacgctg
     3421 ctgtgtcata acaatcctgg tcttttttta gacatatttt ttctttgtcc ggaaagtact
     3481 tttccggata tggcgtcaga atttattgat tcaccaacgg caactttctc tggataaaga
     3541 attccggata ccatgttctg cacatactga gtgagtcgat gagcggaatg ttgtccgata
     3601 tattttgcat gtagccgtat attccgtttc actctgctgt caccgcatcc cgtatagagc
     3661 agcctgaaat cgctcagtgt tcccagtcca agtttcatta aagcccgtgt ggcaatcgca
     3721 tgcagggtct ttgaactgcg ttgttcataa cgggcaatct cactgaaact catgccctgc
     3781 atcagaatct taagcacgct caactcccgt gcagtcagga gccagaaatc gctcatatcg
     3841 ccgggcaaag gacgtagtaa ccagcggata atttgattct gataacgact cgctgtcagc
     3901 atggtgccga tgcgtgggaa gccgttatac agacttgcag caggagcatt atcgaaccag
     3961 tcggagtcac cggtcaggca gacctgccat tgcccggcat cactccgcaa gcggacaaga
     4021 caagccagaa gaaccaactc tcccgaagga agcgatggga tatcagctaa caggtagcca
     4081 caggaggtct ttaaaacgaa ttcaacctct gtagtgacgt tgctgtacac ccgcacgata
     4141 tacctgtccg ttgggatgag agactccagt gccagcctgg tgaactgaca ggcactgata
     4201 ataaccagag gcttcttcat tcagactcgt ccacgccatt gctacaaaac ctgacatttt
     4261 gattcagaag gtactgtttt aacgaggctt ttttttccac acccctttaa atcgagcagc
     4321 attactggct gtgtatctga cagtatgctg aatatttcga tgacccagat aatcctgaag
     4381 tagtcgggta tcgacaccat tgtcagccag agcaaatcca caggcatgac gtaacatatg
     4441 aggatgtgct tttacagcta atcctgcacg tcttcctgcc tcacgaatga tcgaaaaaaa
     4501 atgttgtctg ctgatagggc ggcgtttccg ggagagaaaa agccagtctc cttcaactcc
     4561 attttcaatt aacttcctct gcttaagcca caacttgatc aaattatatt catcaggaag
     4621 aagaggatgt gttgtcgaga agccattttt aatacgccgg atattaagtt gttttcctga
     4681 tgcatcaata tcggataaac gtaaatcaag aagttcactg gccctaaaac cgtgaatgaa
     4741 agccatcata attagacagc gatttctttc aggaaaggac atgctctggg ctgcatccat
     4801 cagcctgtag acttcatcct gtgtgagata ttttctcgtc atacgccacc gtgtacagtt
     4861 gtaaaaaaaa gaggcacacc cagataagtg tgcctgcaat cagaacgtta tcgatgtaat
     4921 ttcattatct cattaataag attt
//
LOCUS       DAPHCV010000205         4773 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.207, whole genome shotgun sequence.
ACCESSION   DAPHCV010000205 DAPHCV010000000
VERSION     DAPHCV010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.207"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            192..902
                     /locus_tag="RH033_004523"
     CDS             192..902
                     /locus_tag="RH033_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDV2993601.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            944..3169
                     /locus_tag="RH033_004524"
                     /pseudo
     CDS             944..3169
                     /locus_tag="RH033_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            3204..3707
                     /locus_tag="RH033_004525"
     CDS             3204..3707
                     /locus_tag="RH033_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV2993602.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            3767..4681
                     /locus_tag="RH033_004526"
     CDS             3767..4681
                     /locus_tag="RH033_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2993603.1"
                     /translation="MGKTISMKLLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVNVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT         1376 a         1036 c         1040 g         1321 t
ORIGIN      
        1 gtagttaaat ttactgttat ttaaaaaatt tactttgtag caaggttttc ctattaaaag
       61 agaaccaaca tttattacaa acgcattaag tctggaaacc cagcgagtcg ccaatgtatt
      121 agcaacattt accgattgtc tgatgtaacg gtttccccgc ttatcctctt aacacatgga
      181 gaatgcatca gatgcaaact acgcgtaccc cctactctat ttcctttatg gcgacagtgt
      241 tacttttatt gctctttgct tgccatagca ccgttgccaa cgcagctgtc gcactgggtg
      301 cgacgcgggt aatttatccg gcaaaccaga aacaggtttt attgcctgtc acgaataatg
      361 atcctgccag tgtctatctc attcagtcat ggatagaaaa tgcgggtgac caaaaagaca
      421 cccagtttgt cattacgcca ccgctttttt ccatgcaagg taaaaaagag aatacgctgc
      481 ggataatcaa cgccactaat catcagttac caggcgaccg tgaaagcctg ttctgggtta
      541 atgtcaaagc tattcctgct atggaaaaag atcagaagaa tgagaataca ttacagctgg
      601 caattattag ccgcataaaa atgttttatc gccccacgaa ccttgcaatg gcacctgaag
      661 aagcgcctgc aatgcttcgg tttcgtcgca gcggtagcaa acttactctt attaatccaa
      721 cgccctactt cattaccgta acgaatatga aagcaggaaa tagcaatctc ccaaacacca
      781 tggtgccgcc aaaaggtgaa gtatcggtgg atatcccgca tgctgcaacc ggggacatca
      841 gttttcagac gatcaatgat tacggtgcat taactccgcg tattaaggca accatgcagt
      901 aagccagtga cctaattaat aataaactca atcggaatta ataatgacag cctttcgtgc
      961 cgcatttaag gcatatcgta tgcatcaggt gttactactg cctcgctttg cacggcttac
     1021 cattgccctt ggcttagcaa ctgcggtatt tcccgttgac gcagagtatt attttaatcc
     1081 acggttttta agcaatgatc ttgctgaatc tgtcgattta tccgccttta ctaagggacg
     1141 cgaggcacca cctggtacat atcgggtcga catttatttg aacgacgaat ttatgactag
     1201 tcgggatatt acttttattg cagatgataa taacgcagat ctcatcccat gcctgagtac
     1261 agatcttctt gtcagtcttg gcataaaaaa atctgcatta ttagacaata aggaacattc
     1321 ggcagaaaaa catgtgtcgg acaacagcgc atgcacacca cttcgggatc gtttggctga
     1381 cgcatcatct gagtttaatg ttggtcagca acatctatca ctctccgttc cgcaaattta
     1441 tgttggcaga atggctcgcg gctatgtttc tccggatctg tgggaagaag gaataaatgc
     1501 tgggctacta aactatagtt ttaatggtaa ttctattaat aatcgtagta accataatgc
     1561 aggaaaatcc aactatgcat atttgaattt acagagtggc atcaacattg gtagttggcg
     1621 actgcgcgat aactcaacgt ggagttataa cagtgggagt agcaattcat ctgacagcaa
     1681 taaatggcag catatcaata cgtcggctga acgtgacatt attcccttac gctcacgctt
     1741 aacggtaggt gatagttata ccgatggcga tatttttgat agtgtaaact ttcgtggcct
     1801 caaaataaat tcaacagaag cgatgttgcc cgatagccaa catggtttcg ctccggtgat
     1861 tcatggtatt gcccgcggca ccgcacaagt gagtgtaaaa cagaatggat acgatgttta
     1921 tcagactact gtcccacccg gcccttttac tattgatgat atcaactctg cggccaatgg
     1981 tggtgatctg caagtaacca taaaagaggc agacggcagt attcagacat tatatgttcc
     2041 ttattcgtct gttccggttc tccaacgtgc tggatatacg cgttatgcgc ttgccatggg
     2101 ggaatatcgt agtggaaata acctgcaaag ctcccccaag ttcatacaag gtagcttgat
     2161 gcatggactg gaaggaaact ggacacctta tggcggaatg caaattgcag aagattatca
     2221 ggccttcaac cttggtattg gtaaagattt aggacttttt ggtgcctttt ctttcgatat
     2281 cacgcaggcc aatacgacac ttgcagatgg cacccgtcac agcgggcaat cgattaaatc
     2341 cgtctacagc aaatccttct accagacggg aaccaatatc caggtcgcag gatatcgcta
     2401 ttctacgcaa ggtttttata acttatccga cagtgcctac agtcgaatga gtggttacac
     2461 cgtcaagcct cctaccggag acagcaatga gaagacacaa tttattgatt attttaatct
     2521 gttctacagt aagcgtggtc aggaacaaat aagcatctct cagcagcttg gaaattacgg
     2581 tacgacattt ttcagtgcca gtcgccaaag ttactggaac acgtcacgca gcgaccagca
     2641 aatatcattt ggattaaatg tgccgtttag tgatattacg acttcgctga attacagcta
     2701 ttccaataat atatggcaaa acgatcggga tcatttactt gcttttacgc ttaatgttcc
     2761 cttcagtcat tggatgcgta cagacagtca gtcggcattt cgtaattcaa acgccagtta
     2821 cagtatgtca aacgatttga aaggcggcat gaccaatcta tcgggagttt atggcactct
     2881 gctgccggat aataacctga attatagtgt tcaggtcggt aacacccacg ggggtaatac
     2941 atcgtctggc accagtggtt acagttctct taattatcgt ggggcttacg gtaatacgaa
     3001 tatcggttac agtcggagtg gtgacagcag ccagatttat tacggaatga gtggtggcat
     3061 tattgctcat gctgatggca tcacctttgg acagccgctg ggcgacacaa tggttctggt
     3121 taaggctcct ggcgctgata atgtcaaaat agagaaccag accggtattc tttacccttg
     3181 aatttcaata atcagagtca gatatgaaaa gacttcacaa gaggttcctg ttagctacgt
     3241 tttgcgcgtt attcacagca actctccagg ccgccgatgt cactatcact gttaatggtc
     3301 gggtagtcgc taaaccctgc actattcaaa ccaaagaagc taacgttaat ctcggggatc
     3361 tttatacacg caatctgcaa caacctggtt ctgcatcagg ctggcacaat attactttgt
     3421 cattaaccga ttgtccggtt gaaacaagtg cagtgacggc aatcgtgaca ggttcaactg
     3481 acaatacggg ttattacaaa aatgaaggta ctgccgaaaa tattcagata gagctaaggg
     3541 atgaccagga tgcgacgtta aaaaatggcg atagcaaaac ggttattgtt gatgagatca
     3601 ctcgtaatgc acagtttcca cttaaggcaa gagctatcac ggtgaatgga aacgcaagcc
     3661 agggaacgat cgaggcgcta atcaatgtga tctacacctg gcaataacgt cgattcgatt
     3721 ttcttagcct cctttttaaa tcaatttttt aaccttagga tatatcatgg gtaaaacaat
     3781 aagtatgaag cttctgttcg gtatttatct tctattaatg gcaggcaaag tttttgcctt
     3841 cagttgtaat gtcgatggcg ggtcaagtat tggagcaggt acaacctctg tttatgttaa
     3901 tctcgaccct gtaatacaac cgggccagaa tctggttgta gacttgtctc agcatataag
     3961 ttgctggaat gattacggcg gctggtacga caccgatcat ataaacctgg tacaaggctc
     4021 agctttcgca ggctccctac agtcatataa aggatcactg tactggaata atgtgaccta
     4081 ccccttcccg ttgacgacaa ataccaacgt attggatatt ggtgataaga caccgatgcc
     4141 attaccgtta aaactctata ttaccccagt cggagcggcc ggtggagttg tcataaaagc
     4201 aggggaagtt attgcccgta ttcatatgta caaaattgcc acgttaggaa gcggaaatcc
     4261 acgtaatttt acgtggaata ttatttcaaa taacagtgtt gttatgccaa caggcggttg
     4321 caccgttgat agtcgtaatg tcaacgtgaa tctcccggac tttcctggaa gcgcagaaat
     4381 cccccttggc gtatattgct caagtgaaca aaagctctca ttttatcttt cgggtgctac
     4441 cactgacagt gccagacaag tgtttgctaa cacagcacca gatgcaacaa aagcatcggg
     4501 agtgggtgtc tctctgatgc gtaatggaaa aatactggct acgggagaaa atgtttccct
     4561 cggaacagta aataagagca aagtacctct gggtttaagc gcaacctatg gacaaacagg
     4621 aaacaaagtt tcagccggaa ccgtccagtc agtcattggt gtcactttta tctatgaata
     4681 attgtgattt tgacattagt ctatgcgttc cattcacgaa aattgtgcgg ataattattt
     4741 tgtgaaggct attatcctac acttgtaaga aaa
//
LOCUS       DAPHCV010000206         4728 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.208, whole genome shotgun sequence.
ACCESSION   DAPHCV010000206 DAPHCV010000000
VERSION     DAPHCV010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.208"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(74..835)
                     /gene="kduD"
                     /locus_tag="RH033_004527"
     CDS             complement(74..835)
                     /gene="kduD"
                     /locus_tag="RH033_004527"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HDV2993604.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(865..1701)
                     /gene="kduI"
                     /locus_tag="RH033_004528"
     CDS             complement(865..1701)
                     /gene="kduI"
                     /locus_tag="RH033_004528"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HDV2993605.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDKLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(1988..3169)
                     /locus_tag="RH033_004529"
     CDS             complement(1988..3169)
                     /locus_tag="RH033_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDV2993606.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVS
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3424..4653
                     /locus_tag="RH033_004530"
     CDS             3424..4653
                     /locus_tag="RH033_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDV2993607.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         1227 a         1205 c         1047 g         1249 t
ORIGIN      
        1 atatggatta tgatgatcta caggtataaa aaaccctgcc atgcggcagg gtcataaaag
       61 taagaggaat gaattaacgc gccagccaac cgccatccac agcaatggta taaccattta
      121 cataatctga agcgctggag gcaagaaaca ctaccggccc catcaggtca ctcggcagtc
      181 cccaacggcc agctggaatg cggtcgagaa tttccgcgct acgttgttca tctgcccgca
      241 gttgttgagt attgttggtc gccatgtaac ccggagctat cgcattaaca ttaatgttgt
      301 gtttagccca ttcattcgcc atcaatcgcg tcacgcccat cacgccgctt tttgatgcgg
      361 tataagaagg cacacggatc ccgccctgga aggagagcat tgacgcgata ttgataatct
      421 tgccgccatt gccttgcgcg ataaagtgtt tcgccgctgc ctgagacatg aagaatacgc
      481 tcttgatatt caggttcatg acatcgtccc agtctttttc gctgaactcg agagcatctt
      541 cgcggcgaat caatccggcg ttattcacca ggatatcaat atgaccaaac tcagctaccg
      601 cgcgatccag cagtgctgga atgccatcaa tctttcgcag atcggcggtc aggcttaaaa
      661 aacgacgccc cagcgccgtg acctgcttga tggtttcagt cggttcaacg atgttaatgc
      721 caacaatgtc acagcccgct tgcgccagcc ccaacgccat cccctggccc agcccagtat
      781 cacaaccagt gacgaccgca actttacctt cgagagaaaa tgcacttaaa atcataacaa
      841 taccttattc gtttatgccc acaactagcg caaatcttta acggccacat ggtccatatc
      901 atcaaaaacc tggttttcac cgaccatgcc ccagataaag gtataagctt tggttccgac
      961 gccggaatgg atcgaccagc tcggggagat cactgcctgc tcgttatgca tcacaatatg
     1021 acgcgtttct tgcggctgcc ccatcatgtg gaaaacgcag gcgtcatcat ccatattgaa
     1081 atagaaataa acttccatcc ggcgctcgtg ggtgtgacac ggcatggtgt tccacaagtt
     1141 acccggagcc agctccgtca gccccatact caattggcag gtttccagta catccgggac
     1201 aaaatatttg ttaatcgtgc gacggttact ggtgagttta tcgcctaacg tgactggaga
     1261 tacttcgtcc ggtgtgactt ttttggtggg atacgtcgta tgcgcaggtg cgcaattgta
     1321 ataaaacttc gccggagtgg cggtatcaat actggcaaag acaacttctt ttgcaccttt
     1381 accaacatac agggcgtcgc ggtgaccgat ttcatagcat tgaccatcga cagtaatcgt
     1441 accggcaccg ccaatattga taacacctaa ctcgcgacgt tccaggaaat agcttacgcc
     1501 gagttgttta ccaacttccc cgccaacgga aaccgtttta gttaccggca taatgccgcc
     1561 aacaattata cggtcaatgt ggctgtaaac catggtgtac tcatcggcga caaatacctt
     1621 ttcaaccaaa aattcattgc gcagcccctg ggtatccagc gtttttgcgt gcgcactgtg
     1681 gatgctctgt cttacgtcca catcaacctc cgataatcag tgaagcgtga aaatagaaaa
     1741 cgatagtgaa acactgtttc gttttctcta taaggggatg atatgcccac gggatagggt
     1801 tttcaattaa aaataaaaca atgtttcatt tttaagttaa ggactgaaaa aagtgcagtg
     1861 tcgatcacga atatgtcatg agaatggaaa aatcagggag ccctgaaaat gtatgtcgac
     1921 tcgccagcca ccattattca acagattaat gacggctgat atttatcggt aaggaggaaa
     1981 tgcagagcta ttcgtcacgt tcaatggtca atgccacgcc ctggcccccg ccgatacaaa
     2041 gcgtagccag tcctttgcgg gcattacgtt tcaccatttc atgaaccaga gaaacgagga
     2101 ttcggcaacc ggaagcgcct attgggtgac cgagtgcgat cgcgccacca ttgacattga
     2161 cacgacgctc atcccattca agcatcttgc caaccgaaag cgcctgtgca gcaaacgctt
     2221 cattagcctc gataagatcg acatcagcca actgccagcc tacacgctcc aggcaacggc
     2281 gggtcgcata caccggcgca attcccatca atgccggatc tacaccaacg ctggcaaatg
     2341 cgcggatgcg ggccagcacg ggtaaattca acgctcgtgc tttggcttcg ctcatcatca
     2401 ttactgcagc tgcgccatcg tttatggatg atgcattacc cgctgtcaca gaaccgagac
     2461 tatcaaatga aggatttaaa cgggctaagc cttctgcact ggcgtcagtg cgtggctgct
     2521 catcggtatc aacaaccaac gtctgaccgt tactttgggt cattaccggg acgatctcat
     2581 ctttaaatcg tccagcgtca atcgccgctc gcgctttttg ttgcgagcta agtgcgtaag
     2641 catcctgcaa ctgacggctg atgccatatt cgcgagccag attttcggcg gtgacaccaa
     2701 tatgataatc attgaaggca tcccacaagc cgtcatgcac cagactatca accaactggc
     2761 tattgccaag ctgtgcaccg gtgcggctat cagtcagaac gtgtggtgcg cggctcatgt
     2821 tttcctgacc accggcgatg acaatatcag cctcgccaca ctgtatcgcc tgagtagcca
     2881 gatgcagtgc tttaagcccg gaaccgcaaa cgtcattaat agtgattgca gaaacgctgt
     2941 taggaagacc acctttaata gccgattgcc ttgccggatt ctgccctgcc cctgcagtca
     3001 acacctgacc aagaatcact tcatccaccg catatgcaga aacaccggta cgttctatta
     3061 atgctttcac gaccaggctg ccaagttcca cggcggaatg acccgctaac gcaccacgga
     3121 agcagccgat aggtgtccgt aacgccccga caatcacaac gtctttcatc acaacctcgc
     3181 accaattaac agtgcaatag taaatgattg ttaattacag ttatcttaat tgtttaaaaa
     3241 aagtgactat tatcacaaag gaaatacgcc tgagtagcag tcagagacat aactggcacg
     3301 taaggtttgc aaccactaac ccaccaatag agaggtagat agggcgttaa tctcccatac
     3361 ttaacctggt ttatggtaaa ttgccctcca ttttgtttaa tttgtagatg atacgttcag
     3421 ataatgtcta atatttggtc aaaagaagaa actctgtgga gtttcgcgct ctacggcaca
     3481 gccgttggtg caggcacgct cttccttcct attcagttag gttcggcggg ggctgtggtc
     3541 ctgtttatta ctgctctggt cgcctggcct ttaacatatt ggccacataa agccttatgc
     3601 cagttcatcc tctcatcaaa aacatcagca ggtgaaggga taacgggcgc ggtaacacac
     3661 tactatggca agaagattgg taatctgatt accacgctgt atttcatcgc cttttttgtc
     3721 gtcgtgttga tatatgccgt ggcaattacc aactcactta cggaacagct ggcaaagcat
     3781 atggttattg atcttcgcat ccgtatgttg gtgagtctgg gggttgtatt aatcctgaat
     3841 ctcatttttc tgatgggacg ccatgccact attcgggtaa tgggattttt ggtattccca
     3901 ttgattgcct atttcttatt tctttctatt tacctggtcg gtagttggca acctgatcta
     3961 ttaacaaccc aggtagagtt caatcagaat acccttcacc agatatggat atcgattccc
     4021 gtgatggttt tcgcctttag ccatacgccc attatttcta cgtttgccat agacagacgt
     4081 gaaaaatatg gcgaacacgc tatggataaa tgcaaaaaaa ttatgaaagt cgcttatctc
     4141 atcatctgca taagtgtact gttctttgtc tttagctgcc tgctttctat tccaccttcg
     4201 tatattgaag cggctaaaga agaaggggtt accattttat cggcgctttc tatgctgccg
     4261 aacgccccag catggttgtc aatttccggg attattgtcg cagtagttgc gatgtcaaaa
     4321 tcattcctgg gtacgtactt tggcgttatt gaaggtgcca cagaggtcgt caaaacaaca
     4381 ttacagcagg ttggtgtaaa gaaaagtcgt gcatttaacc gcgcactatc aattatgttg
     4441 gtatcgctga ttaccttcat tgtttgttgc attaacccga acgcgatttc gatgatttac
     4501 gcgatcagcg gcccgctcat tgccatgata cttttcatca tgcctacgct gtcaacgtat
     4561 ctcatcccgg cgcttaaacc ctggcgttcc atcggaaatc tgattacgct gatcgtgggt
     4621 atcctgtgcg tatcggtaat gttctttagc taatatccgt tgtggatggt tcaaaattgc
     4681 ttcaattttg aaccatccca gttttcataa tgttcatttg ttattatt
//
LOCUS       DAPHCV010000207         4725 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.209, whole genome shotgun sequence.
ACCESSION   DAPHCV010000207 DAPHCV010000000
VERSION     DAPHCV010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.209"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            179..1141
                     /gene="tauA"
                     /locus_tag="RH033_004531"
     CDS             179..1141
                     /gene="tauA"
                     /locus_tag="RH033_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV2993608.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            1154..1921
                     /gene="tauB"
                     /locus_tag="RH033_004532"
     CDS             1154..1921
                     /gene="tauB"
                     /locus_tag="RH033_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HDV2993609.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLENGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKGIQGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGVEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGEPSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1918..2745
                     /gene="tauC"
                     /locus_tag="RH033_004533"
     CDS             1918..2745
                     /gene="tauC"
                     /locus_tag="RH033_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HDV2993610.1"
                     /translation="MSVLINEKLHSHRLKWRWPLSRQVTLSIGTLAVLLTVWWAVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRILLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            2742..3593
                     /gene="tauD"
                     /locus_tag="RH033_004534"
     CDS             2742..3593
                     /gene="tauD"
                     /locus_tag="RH033_004534"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HDV2993611.1"
                     /translation="MSERLSITPLGPYIGAQITGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIQTPPAGAILAAKELPSTGGDTLWASGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEAEHQRWREAVAKNPPLLHPVVRTHPVTGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     IPFYRAG"
     gene            complement(3633..4607)
                     /gene="hemB"
                     /locus_tag="RH033_004535"
     CDS             complement(3633..4607)
                     /gene="hemB"
                     /locus_tag="RH033_004535"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDV2993612.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         1057 a         1224 c         1369 g         1075 t
ORIGIN      
        1 taaaatgaag tgcagtaact tactgaattg tcagagtggg tgtaattgca tatttaatct
       61 ttcctttgcc actttttcgc taagaataaa atcatctgtg cgataacaac taattctttt
      121 aatgaatgtt tttattcctg aatactgctc ccataacaag acaggggagc agacaatcat
      181 ggcaatttca tcgcgtaaca cacttcttgc cgcactggca ttcatcgctt ttcaggcaca
      241 ggcggtgaac gtcactgtgg cgtatcaaac ttcagccgaa ccggcgaaag tggctcaggc
      301 cgacaacacc tttgctaaag agagcggggc gagcgttgac tggcgtaagt ttgacagcgg
      361 agccagcatc gtgcgggcgc tggcttcggg cgacgtgcaa atcggcaacc tcggttccag
      421 cccattagcg gtggcagcca gccaacaagt gccgattgaa gtcttcttac tggcgtcaaa
      481 actgggtaac tccgaagcgc tggtggtaaa gaaaactatc agcaaacccg aagatctgat
      541 tggcaaacgc atcgccgtac cgtttatctc caccacccac tacagcctgc tggcggcgct
      601 gaaacactgg ggtattaaac ctgggcaagt ggagattgtg aacctgcagc cgcccgcgat
      661 tatcgctgcc tggcagcggg gagatattga tggtgcttat gtctgggcac cggcggttaa
      721 cgcgctggaa aaagacggca aggtgctgac cgattctgaa caggtcgggc agtggggtgc
      781 gccgacgctg gatgtttggg tagtgcggaa agattttgcc gagaaacatc cagaggtcgt
      841 gaaagcgttc gccaaaagcg ccatcgatgc tcagcaaccg tacattgcta acccagacgc
      901 gtggctgaaa cagcctgaaa acatcagcaa actggcgcgg ttaagcggcg tgcctgaagg
      961 tgacgttccg gggctggtga aggggaatac ctatctgacg ccgcagcagc aaacggcaga
     1021 actgaccgga ccggtgaaca aagcgatcat cgacaccgcg cagtttttga aagagcaggg
     1081 caaagtcccc gccgtggcga atgattacag ccagtacgtt acctcgcgct tcgtgcaata
     1141 aaaggaggcg cagatgctgc aaatttctca tctttacgcc gattatggcg gcaaaccggc
     1201 actggaagat atcaacctga cgctggaaaa cggcgagctt ctggtggtgc tggggccgtc
     1261 tggctgcggc aaaactacgt tgctgaattt gattgctggt tttgtgcctt atcagcatgg
     1321 cagcattcaa ctggcgggta agggtattca gggaccggga gcagagcgtg gcgtagtttt
     1381 tcagaatgaa gggctgttac cgtggcgcaa tgtacaggac aacgtggcgt tcggcctgca
     1441 actggcaggt gtagagaaaa tgcagcgact ggaaatcgca caccagatgc tgaaaaaagt
     1501 cgggctggaa ggcgcagaaa aacgctacat ctggcagctt tccggtggtc aacgtcagcg
     1561 ggtggggatt gcccgcgcgt tggcggcaaa tccccagctg ttattactcg acgaaccgtt
     1621 tggtgcgctg gacgccttca cccgcgacca gatgcaaacc ctgctgctga aactctggca
     1681 ggagacgggc aagcaggtgc tgttgattac ccacgatata gaagaagcgg tgtttatggc
     1741 gactgaactg gttctgcttt cacccggccc aggccgtgtg ctggagcggc tgccgctcaa
     1801 ctttgctcgc cgctttgttg ccggggaacc gagccgcagc atcaagtccg atccacaatt
     1861 catcgccatg cgcgaatatg ttttaagccg cgtatttgag caacgggagg cgttctcatg
     1921 agtgtgctca ttaatgaaaa actgcattcg catcggctga aatggcgctg gccgctctcg
     1981 cgtcaggtga ccttaagcat tggtacgtta gcggttttgc tcactgtatg gtgggcagtc
     2041 gcggcgctgc aactgattag cccgctgttt ttgccgccac cgcaacaggt acttgcaaaa
     2101 ctgctcacca ttgccgggcc gcagggattt atggacgcca cgctgtggca gcatctggca
     2161 gccagcctga cgcgcatttt gctggcgcta ctcgcggcgg tgttgatcgg tattccggtc
     2221 gggatcgcca tggggctaag cccgacggtg cgcggcattc tggatccgat aatcgagctt
     2281 tatcgtccgg tgccgccgct ggcttatttg ccgctgatgg tgatctggtt tggtattggc
     2341 gaaacctcga agatcttact gatctattta gcgatttttg cgccggtggc gatgtcggcg
     2401 ctggcggggg tgaaaagcgc gcagcaggtt cgcattcgtg ccgcccagtc gctgggtgcc
     2461 agccgtgcgc aggtgctgtg gtttgtcatt ttgcccggcg cgctgccgga aatcctcacc
     2521 ggattacgca ttggtctggg tgtgggctgg tctacgctgg tggcggcgga gctgattgcc
     2581 gcaacgcgcg gtttaggatt tatggttcag tcagcgggtg aatttctcgc gactgacgtg
     2641 gtgctggcgg ggatcgcggt gattgcgatt atcgcctttc ttttagaact gggtctgcgc
     2701 gcgttacagc gccgcctgac gccctggcat ggagaagtac aatgagtgaa cgtctgagca
     2761 ttaccccgct ggggccgtat attggcgcac aaattacggg ggccgactta acgcgcccgt
     2821 taagcgataa tcagtttgaa cagctttacc atgcggtgct gcgccatcag gtggtgtttc
     2881 tgcgcgatca agctattacg ccgcagcagc aacgcgcgct ggcccagcgt tttggcgagc
     2941 tgcatattca ccctgtttac cctcatgctg aaggggttga cgagatcatc gtcctggata
     3001 cccataacga taatccgccg gataacgaca actggcacac cgatgtgaca tttattcaaa
     3061 cgccacccgc aggggcgatt ctggcggcga aagagttacc ctcgaccggc ggcgatacgc
     3121 tctgggccag tggtattgcc gcatatgagg cgctctctgt tcccttccgc cagctgttga
     3181 gcgggttgcg tgcggagcat gatttccgta aatcgttccc ggaatacaag taccgcaaaa
     3241 ccgaggctga gcatcaacgc tggcgtgagg cggtcgcgaa aaatccaccg ttgctacatc
     3301 cggtggtgcg aacgcatccg gtgaccggta aacaggcgct gtttgtgaac gaaggtttta
     3361 ccacacgaat tgttgatgtc agcgagaaag agagcgaagc cttgctgggc tttttgtttg
     3421 cccacatcac caaaccggag tttcaggtgc gctggcgctg gcaaccaaat gatattgcga
     3481 tttgggataa ccgcgtaacc cagcactatg ccaatgcgga ttacctgcca cagcggcgga
     3541 taatgcatcg ggcgacgatt cttggggata ttccgtttta tcgggcgggg taagggggaa
     3601 atgccgggtt ggataacccg gcataatgat cattaacgca gaatcttctt ctcagccaaa
     3661 tccagcgcaa agtagctgaa aatcagatcc gcacccgcac gcttaatcga acctaagctt
     3721 tcgagcacga ctttctcttc atctatagca cccgccagcg cggcgaactt aatcatcgcg
     3781 tactcaccgc tcacctgata cgcgccaatc ggcaattcag tacgttcacg cagctcacgc
     3841 acgatgtcga ggtatgctcc ggcaggttta accatcaggc agtctgcgcc ctgggcttca
     3901 tccagcagcg actcacgaat cgcctcacga cggttcattg ggttcatctg atagcttttg
     3961 cggtcgcctt ttaatgcgct tccggcagct tcacggaacg ggccataaaa ggaggaggcg
     4021 aacttggtcg aatacgacat aatcgccgta tctttaaagc ccgcagcgtc cagcgcctgg
     4081 cgaatcgcct gtacctgacc atccatcgca gcggaagggg cgatgaaatc tgcgcctgca
     4141 gcagctgcaa ccacggcttg cttgcctaaa ttttccagag tcgcgtcgtt gtcgacgcca
     4201 tgctcgcaca gcacaccgca gtgaccgtga gaagtgtatt cacagaaaca agtgtctgac
     4261 atgacgatca tttctggcac agtctgcttg cagatgcgcg acatacgcgc caccagtcca
     4321 tcttcccgcc aggcgtcgct gccggtttca tcggtatggt gagagatgcc gaaagtcatc
     4381 acggaacgaa taccggcgtt ggcgatgcgt tcaatttcgc gtgccagatg tttctctgga
     4441 atgcgcatca cgcctggcat ggcttcaacg gctttgtagt cgtcaatttc ttcttcaaca
     4501 aagatcggca acaccaggtc gttaaggcta agtgttgtct cttcaaacat agcgcgcagc
     4561 gcaggagatt tgcgcaggcg acgagggcgt tggattaaat ctgtcatggt ctgcctgatg
     4621 tttgtggaat cgagggacat agtatacctg aagcaggctg ggtgtgtttt acgaaagttg
     4681 tcgcgatgtt gacaagaaga gaatgaaaaa gaggtcagga catct
//
LOCUS       DAPHCV010000208         4700 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.210, whole genome shotgun sequence.
ACCESSION   DAPHCV010000208 DAPHCV010000000
VERSION     DAPHCV010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.210"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            103..1143
                     /gene="pstS"
                     /locus_tag="RH033_004536"
     CDS             103..1143
                     /gene="pstS"
                     /locus_tag="RH033_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDV2993613.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            1230..2189
                     /gene="pstC"
                     /locus_tag="RH033_004537"
     CDS             1230..2189
                     /gene="pstC"
                     /locus_tag="RH033_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HDV2993614.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            2189..3079
                     /gene="pstA"
                     /locus_tag="RH033_004538"
     CDS             2189..3079
                     /gene="pstA"
                     /locus_tag="RH033_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDV2993615.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMIAAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            3170..3943
                     /gene="pstB"
                     /locus_tag="RH033_004539"
     CDS             3170..3943
                     /gene="pstB"
                     /locus_tag="RH033_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HDV2993616.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            3958..4683
                     /gene="phoU"
                     /locus_tag="RH033_004540"
     CDS             3958..4683
                     /gene="phoU"
                     /locus_tag="RH033_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDV2993617.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAEKDSDK"
BASE COUNT         1085 a         1204 c         1296 g         1115 t
ORIGIN      
        1 cataaaactg tcataaacct tacatataac tgtcacctgt ttgtcctatt ttgcttctcg
       61 tagccaacaa acaatgcttt atgaatcctc ccaggagaca ttatgaaagt tatgcgtacc
      121 accgtcgcaa ctgttgtcgc cgcgacctta tcgatgagtg ctttctctgt gtttgcagaa
      181 gcaagcctga caggtgcagg tgcaaccttc cctgcgccgg tgtatgccaa atgggctgac
      241 acttaccaga aagaaaccgg taataaagtt aactaccagg gtatcggttc ttccggtggc
      301 gtaaaacaga ttatcgctaa taccgttgat ttcggtgcct ctgacgcgcc gctgtctgac
      361 gaaaaactgg ctcaggaagg cctgttccag ttcccgaccg tgattggcgg cgtagtgctg
      421 gcggttaata ttccaggtct gaagtctggc gaactggtgc tggatggtaa aaccctcggc
      481 gacatctacc tgggcaaaat caagaagtgg gatgatgaag ccatcgccaa actgaatccg
      541 ggtctgaaac tgccttcaca gaacattgct gtagtacgcc gcgcagatgg ctccgggact
      601 tccttcgtct tcaccagcta cctggcgaaa gtgaacgaag agtggaaaaa caacgttggt
      661 actggctcta ccgtaaaatg gccgatcggt ctgggcggta aaggtaacga cggtatcgcc
      721 gcgttcgttc agcgtctgcc gggtgcaatt ggttacgttg aatatgctta cgcgaagcag
      781 aacaacctgg cgtacaccaa actgatctcc gctgatggca aaccggtgag tccgaccgaa
      841 gaaaacttcg ctaatgcagc aaaaggcgca gactggagca aaaccttcgc tcaggatctg
      901 accaaccaga aaggcgaaga tgcatggcct attacctcta ccacgttcat tctgatccat
      961 aaagatcaga agaaaccaga acaaggcacc gaagtgctga agttcttcga ctgggcatac
     1021 aaaaccgggg ctaaacaggc gaacgacctg gattatgcca gcctgccgga tagcgtagtt
     1081 gaacaggttc gcgctgcgtg gaagaccaat attaaagaca gtagcggcaa gccgctgtac
     1141 taataaaact ccaggccggg tacggtgttt tacgccgcat ccggcattac aaaatgactt
     1201 tgtaaacgcg tttaactgaa gagtaactta tggctgcaac caagcctgct tttaacccac
     1261 cgggtaaaaa gggcgacata attttcagcg tgctggtaaa actggcggcg ctgattgtgc
     1321 tattgatgtt gggtggcatt attgtctctc tgatcatctc ctcctggccg agcattcaga
     1381 aatttggtct ggctttcctg tggaccaaag agtgggatgc gccgaacgat atctacgggg
     1441 cgctggtgcc gatctacggt acgctggtga cctcgtttat cgcgctgctg atcgccgtcc
     1501 cggtgagttt tggtatcgcc ctgttcctga ccgagcttgc gcctggctgg ctgaaacgcc
     1561 cgctgggtat cgccattgag ctgctggctg ccattccaag tatcgtttac ggcatgtggg
     1621 gcctgtttat ctttgcgccg ctgttcgccg tttacttcca ggagccggtc ggcaatatca
     1681 tgtcgaatat cccgattgtc ggcgcgctgt tctccggccc ggcctttggt atcggtatcc
     1741 tcgcggcagg cgtgatcctc gccatcatga ttattccgta cattgcggcg gtaatgcgcg
     1801 atgtattcga acaaaccccg gtgatgatga aagagtcggc ctacggtatt ggctgcacca
     1861 cctgggaagt tatctggcgt atcgttcttc cgttcaccaa aaatggtgtt atcggcggca
     1921 ttatgttggg gctggggcgc gcgctcggtg aaaccatggc ggtgaccttt atcatcggta
     1981 acacctacca actcgacagc gcctcgctgt atatgccggg caacagtatc acctctgcgc
     2041 tggccaacga atttgcggaa gcggaatccg gtctgcacgt cgctgcactg atggaactgg
     2101 gcctgatcct gtttgtgatt accttcatcg tcctcgccgc atcgaagttt atgattatgc
     2161 gcctggctaa gaatgagggg gcacgctaat ggctatggtt gaaatgcaaa ccactgcggc
     2221 actggctgaa tcccgccgca aaatgcaggc gcgtcgccgc ctcaaaaacc gtattgcgct
     2281 gacgctctca atggcgacga tggccttcgg tctgttctgg ctgatctgga ttttaatgtc
     2341 caccatcact cgcggtatcg acggtatgtc gctggcgctg ttcaccgaaa tgacgccacc
     2401 acccaatacg gaaggcggtg gtctggcgaa cgccctggcg ggtagcggcc tgttaatttt
     2461 gtgggctacc gtattcggta cgccgctggg cattatggcg gggatttacc tggctgaata
     2521 tggtcgtaaa tcctggctgg cagaagtgat tcgcttcatt aacgacattc tgctctctgc
     2581 gccgtcgatt gtggttggtc tgtttgttta caccattgtg gtggcgcaga tggagcactt
     2641 ctccggctgg gcgggcgtga ttgcgctggc gctgttgcag gtgccgattg ttatccgcac
     2701 caccgagaac atgctgaaac tggtgccgga cagcctgcgt gaagcggctt atgcgctggg
     2761 tacgccgaag tggaagatga ttgccgcgat cacgctgaaa gcgtcggtgt ccgggatcat
     2821 gaccggtatc ctgctggcga ttgcccgtat tgctggtgaa accgcgccgc tgctgtttac
     2881 tgcgctctcc aaccagttct ggagcacgga catgatgcag ccgatcgcca acctgccggt
     2941 gacgatcttt aagtttgcga tgagcccgtt tgcggaatgg cagcaactgg cctgggccgg
     3001 ggtgttgatc attaccctgt gcgtactgct gctgaacatt ctggcgcgcg ttgtttttgc
     3061 gaagaataaa cacggttaac actttgctgg atgcggcgta aacgccttat ccggcctacg
     3121 gtaagcctga ttcgcgaagt gcatcaggca cgatgaggaa aagattgcaa tgagtatggt
     3181 tgaaactgcc ccgagtaaaa ttcaggttcg taatttgaac ttctactacg gcaaattcca
     3241 tgccctgaaa aacatcaacc tggatatcgc caaaaaccag gttacggcgt ttatcgggcc
     3301 gtccggctgc ggtaaatcaa cgctgctgcg taccttcaac aaaatgtttg aactgtaccc
     3361 ggaacagcgt gcggaaggtg aaattctgct cgatggcgac aacatcctga ccaactctca
     3421 ggatatcgcc ctgctgcgtg cgaaagtggg catggtgttc cagaaaccga cgccgttccc
     3481 gatgtccatc tacgacaaca tcgcttttgg cgtgcgtctg tttgagaagc tctcccgtgc
     3541 tgacatggac gagcgcgtgc agtgggcatt gaccaaagcc gcattgtgga acgaaaccaa
     3601 agataaattg caccagagcg gttactctct ctctggtggt cagcaacagc gtttgtgtat
     3661 tgcgcgtggt atcgccattc gcccggaagt gctgctgctc gacgaaccat gttcggcgct
     3721 cgaccccatc tccaccgggc gtattgaaga gctgattacc gaactgaagc aggattacac
     3781 cgtggtgatc gtcacccaca acatgcagca ggctgcgcgt tgttccgacc acacggcgtt
     3841 tatgtacctg ggcgaattga ttgagttcag caacacggac gatctgttca ccaagccagc
     3901 gaagaaacaa acagaagact acatcaccgg tcgttacggt tgattcagga gtgcgttatg
     3961 gacagtctca atcttaataa acatatttcc ggccagttca acgccgaact ggaaagcatc
     4021 cgcacgcagg tgatgaccat gggcggcatg gtggagcagc agctttctga tgcgatcact
     4081 gcaatgcaca accaggacag cgatctggcg aagcgcgtca tcgaaggcga caagaacgtc
     4141 aacatgatgg aagtggcgat cgatgaagcc tgcgtgcgca ttattgccaa acgccagccg
     4201 acggcgagcg acctgcgtct ggttatggtg atcagtaaaa ccattgccga gctggagcgt
     4261 attggcgacg tggcggacaa aatctgccgt actgcgctgg agaaattctc ccagcagcat
     4321 cagccgttgc tggtaagtct ggagtcgctg ggccgtcata ccatccagat gctgcacgac
     4381 gtgctggacg cgttcgcgcg gatggacatt gatgaagcgg tacgtattta tcgtgaagat
     4441 aaaaaagtcg atcaggagta cgaaggtatt gttcgtcagc tgatgaccta catgatggaa
     4501 gattcgcgca ccattccaag cgtacttacc gcgctgtttt gtgcgcgttc aatcgaacgt
     4561 atcggcgacc gctgccagaa tatttgtgag tttatcttct actacgtgaa ggggcaggat
     4621 ttccgtcacg tcggtggtga cgagctcgat aaactgctgg cagaaaaaga tagcgataaa
     4681 taattcacca agtaaatccc
//
LOCUS       DAPHCV010000209         4663 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.211, whole genome shotgun sequence.
ACCESSION   DAPHCV010000209 DAPHCV010000000
VERSION     DAPHCV010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.211"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            697..852
                     /gene="hokD"
                     /locus_tag="RH033_004541"
     CDS             697..852
                     /gene="hokD"
                     /locus_tag="RH033_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HDV2993618.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1069..1320
                     /locus_tag="RH033_004542"
     CDS             1069..1320
                     /locus_tag="RH033_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993619.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALIN
                     FVDGRVTDGFILREGEFVTSLQALKEIGIKAGFSAFAEE"
     gene            1387..1665
                     /locus_tag="RH033_004543"
     CDS             1387..1665
                     /locus_tag="RH033_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993620.1"
                     /translation="MARNVKYYNSDNSPVLACTHGRYSHAFKSEWFQHPPCTAEQAEW
                     LIHSYCRRGFEVKKALSLDYRHWIISVRLPYSERPPRASRTFQQRIWR"
     gene            1667..2716
                     /locus_tag="RH033_004544"
     CDS             1667..2716
                     /locus_tag="RH033_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDV2993621.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESIQIFHNPRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKTLLPFFSNERVIRAAGGVGALSDWLLRHITSCQWPNGDYHH
                     TETVIHRYGTGAMVLCWHCDNQLRDQTSESLELLAQQNLTAWVIDVIRHAISGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKICSVYRESDIVPGEQTATSILKQR
                     TKNLAPLPYAHQQQKPPQEKTVVSINVDPESPESFMKLPKRRRWVKEKYTRWVKTQPC
                     ACCGMPADDPHHLIGHGQGGMGTKAHDLFVLPLCRKHHNELHTDTVAFEEKYGSQLEL
                     IFRFIDRALATGVLA"
     gene            2730..3482
                     /locus_tag="RH033_004545"
     CDS             2730..3482
                     /locus_tag="RH033_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HDV2993622.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGTGCLTITDVMAAQGMVQSK
                     APLGLALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEEIRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCSGTGFYNVLREVVKHYRRGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRFHGVPVYKICGRCNGNRFSRLPTTLARRHVQKLVPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAEAQLRKVTR"
     gene            complement(3657..3859)
                     /gene="cspF"
                     /locus_tag="RH033_004546"
                     /pseudo
     CDS             complement(3657..3859)
                     /gene="cspF"
                     /locus_tag="RH033_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
     gene            4158..4373
                     /gene="cspB"
                     /locus_tag="RH033_004547"
     CDS             4158..4373
                     /gene="cspB"
                     /locus_tag="RH033_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="HDV2993623.1"
                     /translation="MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNYRT
                     LFEGQKVTFSIESGAKGPAAVNVIITD"
BASE COUNT         1274 a          975 c         1196 g         1218 t
ORIGIN      
        1 gttttttcag accgcttctg accacaatgg cctgtgaggt tctgctgcta gtcaacataa
       61 aataacaaaa ccggcaggtg gtggttgttg taaagttatt caatgacatt catggaggct
      121 taagcgaatt atttgtccct ggtcggtatg gtggcaacag cttgttgcga gaatttggct
      181 aatttggact gacttccgct ccttgctcaa agcggactag aaggttagct tgtgtcggac
      241 tttgcgtatt aaaagaagtg ctggtggtga ctagtggttg agccccattt ccacagaaaa
      301 aatcagagaa actataccca atagttgtat tgaatcactg acgagacagc ctcatattca
      361 tcaggactgg tgtacgtcca atacaggagg ttgtggtgct ggttctcaaa tgtgcgctgg
      421 ctattacggc tgtaatggcg atttattgtc ttgctattgt tcttatggat cgcctttctg
      481 actgatttca tattggcgag gtaaaggtag ttaagtagaa tggctgcggg tgcttgaggc
      541 tatctgcctc gggcatgaac accaaaggca gatagagaaa agccccagtt aacattacgc
      601 gtcctgcaag acgcttaaca ttaatctgag gccaatttca tgctagacac atgtaggtta
      661 gcctcttacg tgccggaagg caaggagaag caggctatga agcagcaaaa ggcgatgtta
      721 atcgccctga tcgtcatctg tttaaccgtc atagtgacgg cactggtaac gaggaaagac
      781 ctctgcgagg tacgaatccg aaccggccag acggaggtcg ctgtcttcac agcttacgaa
      841 cctgaggagt aagagacccg gcgagggaga aatccctcgc cacctctgat gtggcaggca
      901 tcctcaacgc acccgcactt aacccgcttc ggcgggtttt tgtttttatt ttcaacgcgt
      961 ttgaagttct ggacggtgcc ggaatagaat caaaaatact taagtagcgc gcagggataa
     1021 gagggatggt cccttaaagg ggagagctaa ttatccggaa ggattctgat gatgaacatc
     1081 gaagaactgc gtaaaatttt ttgtgaagat ggcctctatg ctgtgtgcgt tgaaaatgga
     1141 aatcttgtta gtcattaccg cattatgtgt ttgcgaaaga atggggctgc gttaattaat
     1201 tttgtggatg gtcgagtgac agacggattt atcttgcgcg aaggtgagtt tgtcacttca
     1261 ttacaggcac tgaaagagat cggaataaaa gcaggctttt cagcttttgc agaagaataa
     1321 actcatctac aatcttgcgc ggggctgaac tcccgctgag taacaccgtg ccaccggaga
     1381 aaaccgatgg cacgcaacgt aaaatattac aattctgata attcgcccgt tcttgcctgc
     1441 acgcacgggc ggtattctca cgcattcaag tctgaatggt tccagcaccc tccatgcact
     1501 gcagaacagg ccgaatggct gattcattct tactgcaggc gcgggttcga ggttaagaaa
     1561 gctctcagtc tcgactatcg gcactggata atctctgtca ggctgcctta ttccgaacgc
     1621 ccaccacgtg cgtcccgcac tttccagcaa cggatctgga ggtaacgtgc gggtattact
     1681 tagacctgtt ctggtgcctg agcttgggct ggtggtcctt aagccgggcc gtgaatccat
     1741 acagatattt cataatcctc gagtgctggt ggaaccggaa ccaaaaagca tgcgtaatct
     1801 gccatccgga gtcgttcctg ccgttcgcca gccgctggcg gaagacaaaa cattgctgcc
     1861 gttttttagt aacgaacggg tgattcgtgc tgctggcggc gttggcgcat tgtctgactg
     1921 gctattacgt catattacat cctgccagtg gcctaatggc gattaccatc acactgaaac
     1981 agtcattcac cgttatggta ccggcgcaat ggtgttgtgc tggcactgcg acaaccaact
     2041 gcgtgaccag acatcggaat cactggagct gcttgctcaa caaaatctga cagcatgggt
     2101 gattgacgtc atccgtcacg caataagcgg tacgcaggag cgggaattat ctttggctga
     2161 attatcctgg tgggcggtct gcaatcaggt ggtggatgca ctacctgagg ctgtatcgcg
     2221 tcgttcgctg ggattaccag cggaaaaaat ctgctcggtg taccgcgaaa gcgacatcgt
     2281 accgggagag cagaccgcca ccagcatatt gaaacaacgc acaaaaaatc ttgcaccgtt
     2341 gccttacgcc caccagcaac aaaaaccacc acaggaaaag acggtggtaa gcatcaacgt
     2401 tgatccagag tctccggaat ctttcatgaa gctgcctaaa cgtcgccgct gggttaagga
     2461 gaaatacact cgttgggtta agacacagcc gtgtgcttgc tgcggtatgc cagccgacga
     2521 tccgcatcat ctgattggtc acgggcaggg cggaatggga acaaaagcac atgatctctt
     2581 tgtgttgcct ttgtgcagaa agcatcacaa cgagctgcat acggatacag tggcatttga
     2641 agagaagtat ggctcccaac tggagctgat atttcgtttt atcgatcgcg cgctggcaac
     2701 tggcgtactg gcgtaagtgg agaacgagca tgaaccttga agccttacca aaatattact
     2761 ccccaaaatc tccaaaattg agcgatgacg ctccagcgac aggcaccggt tgtttaacaa
     2821 ttacggatgt aatggcagcg caggggatgg tgcagtcgaa agcaccactt gggttggcct
     2881 tatttctggc aaaagttggt gttcaggacc ctcagtttgc gattgaaggc ctgctaaatt
     2941 acgcgatggc actggataac ccgacattga acaaattgag tgaagaaatc cggttacaga
     3001 ttattcctta cctcgtgaat tttgcctttg ctgattactc caggtctgcg gcaagtaagg
     3061 ctcgctgtga gcattgttca ggtacgggat tttataatgt attgcgcgaa gtggtgaaac
     3121 actacagacg cggggaatct gtaatcaagg aagaatgggt gaaggaacta tgtcagcatt
     3181 gccatggtaa gggcgaagtc agcacagcgt gcagagggtg taagggtaaa gggattgttc
     3241 tggatgaaaa aagaacccgg tttcatggcg taccggtata taagatttgt gggcgttgta
     3301 atggaaaccg atttagtcgt ttaccgacca cgctggcacg acgtcatgtc cagaagctgg
     3361 taccagacct gaccgattat cagtggtata aggggtatgc ggacgtcatt gataaactgg
     3421 taacaaagtg ctggcaggaa gaagcatacg cggaagcgca attgaggaag gtgacgagat
     3481 aaatgatttt tgctgaagat ggcgacatga tgtttgcatt tttcaaaaaa catggataag
     3541 attctctcaa cgatgggctt tgtgtatccg acgtttagaa aaaagtagaa aacccgctta
     3601 taagcgggtt tttgtgcttt aaatggggca atagagatat tgaatctcat cccgggataa
     3661 acattggcag ttgaaggtcc acgcgaacca tttatccggc aaaattccac gcgtaatcct
     3721 gtggtaattt cttctgcatc tcgaagattg agagctgaaa cgtgaagctg ggcatcgata
     3781 cgccatcgga tgggaatata agacctttgc tgcttttgta gtcaaagttt ttgacaattc
     3841 ctgtcatttt aggggacaga aaaactcctt aatactgata acctggtgca ccatacacac
     3901 gttcctggag aaaactactt ttttgatagg gttgaaggtg gctggatgtc taaaataaac
     3961 attgcttcat atgttcaact atgagttaat gactgcgtcg gtttgaagaa cagacgatat
     4021 acgaagtagt ttactaaagc agttctcatt tcaggtgtta ttcacttatt ccttctttga
     4081 gtctctccaa ttaagtacga agtcgtttct gttatgcaaa ccatttatgc cgaaaggctc
     4141 aagttaagga atgtagaatg tcaaataaaa tgactggttt agtaaaatgg tttaacgctg
     4201 ataaaggttt cggctttatt tctcctgttg atggtagtaa agatgtgttt gtgcattttt
     4261 ctgcgattca gaatgataat tatcgaacct tatttgaagg tcaaaaggtt accttctcta
     4321 tagagagtgg tgctaaaggt cctgcagcag taaatgtcat cattactgat taaaattcat
     4381 cgctcgtctg tatacgataa cgaagaaggc tgatgcctga gtagagatac ggacagagta
     4441 gtgaatattg gatctcttta ataaaaagta aggaggtcca atacatgaaa caatggccag
     4501 catatttggc aaaatcttaa tcaggaaaag tatgctaacc attgtggtga agtgcaggtt
     4561 tgctgcatga atagttttac agcagaagct aactgctggc atggcaaaac aaagtgcgta
     4621 agtggatgac tcccacaaaa agcaccacaa tctcaaaccc gct
//
LOCUS       DAPHCV010000210         4659 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.212, whole genome shotgun sequence.
ACCESSION   DAPHCV010000210 DAPHCV010000000
VERSION     DAPHCV010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.212"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            15..1175
                     /locus_tag="RH033_004548"
     CDS             15..1175
                     /locus_tag="RH033_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDV2993624.1"
                     /translation="MHDPIVMEARAELTSAQSRIEQASSAHWPVVTATGSKLLSQSHR
                     YSYDYDTEDILPGIRGEVNIFASGAIEADVRRSESEAEYYHYKMEETKEETIHSFVSL
                     YLDALREKQSIAVLEQSLSRHNAILNDLNTISIHDTGRESELVQAEARRLMVRQQINS
                     RSRVLKTTLGKLSTWTKNPVTEADLENPFSRMTEAKLLTDFTQAPQKGNPSWLASQAD
                     VESKKAALKAQELARYPRVDLTGSVTRDDQQIGVNLSWDLFNRNASYGVTEKAAQIVA
                     ATGRLDSVARMIDETGRLSLITVRQSRGEMETLRRQEQASARVVDFYRLQFQVARKTL
                     IELLNAENELYSVGLSRVQTEDQMLHGMLDYLYSQGMLLKWSGVNLSGEEEK"
     gene            1200..3347
                     /locus_tag="RH033_004549"
     CDS             1200..3347
                     /locus_tag="RH033_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HDV2993625.1"
                     /translation="MKSIFEGLSQITALLGTPVSAETLAAGTVRTDVSGIDFRSVGEF
                     LRSEGFDNHLSRRSPEDIPSLAVPALLLLNAQEAIVVVRIEGAGLARQYHVLQPGGLR
                     KTLSHDALMAVYSGYCWFVQPQAKQDRRSELPEYDMSGAWFWRVLWRFRAYYGQVIVA
                     SFIINFLALVSSLYVMNVYDRVIPNRSWETLWMLSIGVITAILFECAAKLIRSYLTDV
                     AGKKADLIISSALLRRVMAVKMSDRPASSGSYASNLREYESVREFMTSATLLAFVDLP
                     FILLFISVIGLVGGWLAVVPLTLVPLVILAGILLQRPLSRHINASMRESSQRQGLLVE
                     ALDGIETLKLNNAVNWVQRRWDGYTASVARSSIRVKNTSNVIISFSTAIQQINTVLLV
                     ILGTYLIHSDDPHNRITMGALIAAVILSGRALAPLGQIASLATRFQQARLAMQGVNEI
                     VRRPVERESQTHYLTVKDIQGEFHFSAVTFFYKPELPAALKGMSVCIRPGEKVGIIGT
                     IGCGKSTLLKMMVGLYRQNKGQITLDGVAMQQLDPCFVRDKVFLLEQNPRLFLGTLRE
                     NLQLSRLPGSLSDVDMINAAGRFGFDRFIRHHPCGLDMQIGEGGQGLSGGQRQMVALT
                     RMVLGSPRVVLLDEPTTGLDLQTENIVLKGLNHWSRGRTMVVVTHRLQVLNIVDRIIV
                     MNEGRVMMDGPRDEILKKLSGKQQSGESRAITS"
     gene            3408..4655
                     /locus_tag="RH033_004550"
     CDS             3408..4655
                     /locus_tag="RH033_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HDV2993626.1"
                     /translation="MTEKTERNKASSEVVRQSDLALLTDVQAALQQEKHHALFLMVIF
                     LLVLVVTFVIWAWNSPLDEVTRGQGSIIPGSREQVIQTLDPGILKTLEVREGDIVEKG
                     QVLLTLDDTRSSAMLRESEARVNNLEAVRARLRAEAYSESLTFPDDVPADLRERESTV
                     YRLRKTELAQSIAGLKQSKALLDKEIAMTRPIVREGAMSEVELLRMQRQSAELQLQMD
                     EKQNKYLTEAGAELVKTEAELAQAKENMAGRADPVERSRIRAPLRGIVKNIRVNTLGG
                     VVSAGQDIMEIIPLEDQLLIEAYINPRDVAYVRTGMPALVKLTAYDYAIYGGLDGVVT
                     LVSPDTLRDQKRPGDLKLDPNEAYYRVLVTTSNNYLTDRNGKILPVIPGMIASVDIKT
                     GQKSVFQYLIKPITRMKQALQER"
BASE COUNT         1222 a          960 c         1288 g         1189 t
ORIGIN      
        1 ttagggaggc agttatgcac gatcccatag taatggaagc ccgggcggag ttaacttcgg
       61 cacaatcccg catagagcag gcaagctctg cacattggcc agttgtcaca gctacaggaa
      121 gtaaactcct ttcacaaagt caccgttatt cctacgatta tgacactgaa gatattttac
      181 ccggtattcg tggtgaagtg aatatatttg cttcaggggc tattgaggcg gatgtgcgtc
      241 ggagtgagtc agaagccgaa tattatcatt ataaaatgga agaaacaaaa gaggaaacaa
      301 ttcactcttt tgtttcatta tatcttgatg cactcaggga aaaacaatcc attgcggtac
      361 ttgaacagag cctttcccgg cataacgcaa ttcttaatga cctgaatacc atcagtattc
      421 atgataccgg gcgggagtct gagcttgttc aggccgaagc cagaaggttg atggttcggc
      481 agcagataaa ttctaggagc agagtactta aaaccacgct gggaaaactg tccacttgga
      541 caaaaaatcc ggtaaccgaa gctgatcttg aaaatccttt ttctaggatg acagaggcca
      601 aattattaac tgattttaca caggctccac agaaaggtaa cccgtcgtgg cttgccagcc
      661 aagctgatgt tgagagtaaa aaagcggcac tgaaagcaca ggagcttgcc cggtaccctc
      721 gggtggattt aacggggtct gtaacccggg atgaccagca gataggtgtc aatctgtctt
      781 gggacctctt taaccgtaat gccagttatg gtgttacaga aaaagctgcg caaatagtgg
      841 cagctaccgg acgactggac tctgtcgccc gaatgattga tgaaaccggg cgattatctc
      901 tgataacagt cagacaaagt cgaggggaaa tggaaacgct cagacgtcag gaacaggctt
      961 cagccagagt tgtggacttt tatcgtcttc agtttcaggt ggcaagaaaa acactgattg
     1021 aattactgaa tgctgaaaac gaactgtaca gtgtcggact ctcccgggtt cagacggagg
     1081 atcagatgct ccacggtatg ctggattatc tgtattccca gggaatgctc ctgaaatgga
     1141 gcggagtgaa tctttctggt gaagaagaaa aataacgggg ttgaagggtt ggtaattaag
     1201 tgaagagtat ttttgaagga ttatcacaga taacggcgtt actgggtaca cctgtatctg
     1261 ctgagactct ggctgccggt actgtccgta cagatgtatc cgggattgat tttcgttctg
     1321 ttggcgagtt tcttcgtagt gaaggatttg ataatcatct ttcccggcga tctccggaag
     1381 atatcccgtc acttgcggtt ccggcgcttc ttttattgaa cgctcaggaa gctattgtgg
     1441 tagtgcgtat tgagggggcc ggtctggcaa gacagtatca tgtgcttcag cctggaggtc
     1501 tccgtaagac actttctcac gatgcattaa tggccgttta ttctggttat tgttggtttg
     1561 ttcagccaca ggcaaagcag gatcgtcgct cggagctgcc agagtatgac atgagcgggg
     1621 catggttctg gcgggtactg tggcgattca gggcatatta cggacaggtt attgttgcga
     1681 gttttatcat taactttctt gctctggtca gttccctgta tgtaatgaac gtctatgacc
     1741 gggttatacc taatcgttcc tgggaaacgt tatggatgct gagtatcggg gtgattacag
     1801 caattctgtt tgagtgtgct gcaaagttaa tccggagtta tctgacggat gttgcgggta
     1861 agaaggcaga cctgattatc agctctgctc tgctacgtcg ggtgatggcg gtaaaaatgt
     1921 ctgacaggcc ggcatcctct ggttcatacg ccagcaattt gcgggaatat gagtctgtca
     1981 gggagtttat gaccagcgct acgttactgg cttttgttga tctgcctttt attctgcttt
     2041 tcatcagtgt tatcgggctc gtgggaggct ggcttgcggt tgtacctctt acgctggtcc
     2101 cgctggttat tctggcagga atattactgc agcgaccgct ttcccgtcat attaatgcct
     2161 ccatgcggga atcatcgcag cgtcagggat tacttgtgga ggctctggac ggaatagaaa
     2221 ccctgaagct gaataatgct gttaactggg ttcagcgacg ctgggatgga tatactgcaa
     2281 gtgtcgcccg ttcgtccatt cgggtaaaaa ataccagtaa tgtcataatc agcttttcca
     2341 cagcaattca gcaaattaat actgttctgc tggtcattct gggaacgtac cttattcaca
     2401 gtgatgaccc acataaccgt atcaccatgg gggcactgat tgcagccgtt attctttccg
     2461 gccgcgcgct tgcaccgctt ggccagatag catcactggc cacccgtttc cagcaggccc
     2521 gtctggctat gcagggggtg aatgaaatag ttcgccgccc ggtggaacgg gaaagtcaga
     2581 cacattatct gaccgtgaag gatatccagg gggaattcca tttcagcgca gtcacgtttt
     2641 tctataagcc tgaattacct gcagccttga aaggtatgtc tgtctgtatc cggcccggag
     2701 aaaaggtggg cattattggc actatcggat gtggtaaatc gacgctactg aaaatgatgg
     2761 tagggctata ccgtcaaaat aaaggacaaa ttacccttga cggggttgct atgcagcaac
     2821 tggatccctg ttttgttcgg gataaggttt tcctgcttga gcagaatccc cggctttttc
     2881 tgggaactct gcgtgaaaat ctgcagcttt cgcgtcttcc gggaagcctg tcggatgtgg
     2941 atatgattaa tgcagccgga cgtttcgggt ttgaccgttt tattcgtcat cacccatgcg
     3001 ggcttgatat gcagataggg gaaggaggtc aggggctgtc aggaggacag cgtcagatgg
     3061 tcgctctgac gagaatggta ctgggttctc cccgggtagt tctgcttgat gaacccacga
     3121 cgggtcttga tctgcagaca gagaacattg ttctgaaagg gctgaatcac tggagccgtg
     3181 gacgcacaat ggtggtggtg acgcatcgtc tgcaggtgct gaatattgtt gaccgtatta
     3241 tcgtgatgaa tgaaggccgg gtaatgatgg atggtccccg cgatgaaata ctgaaaaaac
     3301 tgtcagggaa acagcagagt ggggagagca gagcgataac ttcatgagcg tcaagcggat
     3361 aagaaataaa gagcatatat gtttaacagt ttgtggaccg caggggtatg acagaaaaaa
     3421 cggaacggaa taaagcgtcg tcagaggtgg ttaggcagtc agacctggcc ctgttgacag
     3481 atgtacaggc agcccttcag caggaaaagc atcatgccct tttcctgatg gtgatttttc
     3541 tgctggtact ggtagtgacg tttgttatct gggcatggaa cagtcctctg gatgaagtga
     3601 cccgtggtca ggggagtatt attcccggca gcagggagca ggttatacag acccttgacc
     3661 cgggtatttt gaaaacactt gaggtcaggg aaggagatat tgttgagaaa ggtcaggtac
     3721 tgctgacact ggatgatacc cgaagctctg ccatgctgag ggaaagtgaa gcccgggtga
     3781 ataacctgga agcagtcagg gcaagactcc gtgcagaagc ttattccgaa tcactgacat
     3841 ttccggatga cgtgcctgca gatttgaggg agagggaaag tactgtttat cgactacgaa
     3901 agactgaact ggcacaaagc atcgccggcc ttaagcagag taaggctctg ctggataagg
     3961 aaatagcaat gacccggcca attgtgcgtg aaggggcgat gtcggaagtc gaactgttac
     4021 gtatgcagcg tcagtcagcg gagctgcagt tacagatgga tgagaaacag aataaatacc
     4081 tgacggaagc tggagctgaa ctagtgaaga cagaagcaga gcttgcacag gcaaaagaga
     4141 atatggctgg cagagccgac ccggttgagc gttcacgtat cagggctccc ctgagaggca
     4201 tcgtaaaaaa tattcgggta aatactttgg gaggcgtggt ttctgcagga caggacataa
     4261 tggaaattat tccgctggaa gaccagttgc tgattgaggc ttatataaat ccacgggatg
     4321 ttgcctatgt cagaacaggg atgccggcac ttgtaaaact gacggcttat gactatgcca
     4381 tttatggtgg tcttgacgga gtggtgacac tggtaagtcc ggatacttta cgtgaccaga
     4441 aacggccggg agatttaaaa ctggatccaa atgaagcata ttatcgagta ctggttacta
     4501 ccagtaataa ttatctgaca gacaggaacg gaaaaatatt gccagttatt cctggcatga
     4561 tagccagcgt ggatataaaa acaggacaga aaagtgtatt tcagtacctg attaaaccaa
     4621 tcaccagaat gaaacaagct cttcaggaac gatgattta
//
LOCUS       DAPHCV010000211         4650 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.213, whole genome shotgun sequence.
ACCESSION   DAPHCV010000211 DAPHCV010000000
VERSION     DAPHCV010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.213"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..627
                     /gene="hprR"
                     /locus_tag="RH033_004551"
     CDS             <1..627
                     /gene="hprR"
                     /locus_tag="RH033_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HDV2993627.1"
                     /translation="VTQGLSEAGYVIDAVSDGRDGLYLALKDDYALIILDIMLPGMDG
                     WQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPFSFSELLARVRAQLRQ
                     HHTLNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLASRAGEIIPRTVIASE
                     IWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSFVAVKK"
     gene            627..1985
                     /gene="hprS"
                     /locus_tag="RH033_004552"
     CDS             627..1985
                     /gene="hprS"
                     /locus_tag="RH033_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HDV2993628.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDSINKIVNRTNVSDDMLN
                     NISATETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVALSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLFLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSEGQGLGLSLVKAIAELHGGSATYHYFNKHNVFRITLPQ
                     RN"
     gene            complement(2093..2944)
                     /gene="hchA"
                     /locus_tag="RH033_004553"
     CDS             complement(2093..2944)
                     /gene="hchA"
                     /locus_tag="RH033_004553"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HDV2993629.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVVSLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPSAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(3536..4594)
                     /locus_tag="RH033_004554"
     CDS             complement(3536..4594)
                     /locus_tag="RH033_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDV2993630.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKDGNKLDLYGKMVGERIL
                     TDRENGEKGDNSQDTSYARVGVKGETQINPELTGYGQFELDLEASNRHNPDQTRLAYA
                     GLSYKDFGSFDYGRNVGVAYDAEAFTDMFVEWGGDSWAGTDLFMTNRTNGVATYRNTD
                     FFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSVTYTIDGFSFTGAYANSDRTDFQS
                     SDSKGEKAEVWALSTKYDPNNVYAAVMYGESHNMNSDDGDVVNKTQNFEAVLQYQFDF
                     GLRPSIGYSYSKALDVAGYKDSDRLNYIEIGTWYYFNKNMNVYTAYQINLLDKSDYVL
                     AHGLNTDDQLAFGIVYQF"
BASE COUNT         1305 a          933 c         1079 g         1333 t
ORIGIN      
        1 gtaacgcagg ggctttccga agcgggttat gtcattgatg ccgtttctga tggcagagat
       61 gggctttatc ttgcgcttaa ggatgattat gcattgatca ttctggatat tatgcttccg
      121 ggtatggatg gctggcagat cttacaaacg ttaagaacag caaagcaaac tccggttatt
      181 tgccttactg caagagattc tgtcgatgac agagtcagag ggctggacag tggggcaaat
      241 gattatctgg taaaaccttt ttcattttct gagttgctgg caagggttcg ggcacaatta
      301 aggcaacatc acactctgaa ttcaacatta gaaatcagtg gcttaagaat ggactctgtt
      361 agtcaaagtg tgagcaggga caatatcagt attacactga cgcgcaagga gtttcagtta
      421 ctttggctac tggcctccag agctggcgag attatcccca gaacggttat tgcgagtgaa
      481 atttggggaa tcaactttga tagtgatacc aatacggtgg acgtcgccat tcgcaggctc
      541 cgcgcaaaag ttgatgatcc ttttcctgaa aagctaatcg ccacaatccg ggggatgggc
      601 tattcattcg tagcggtaaa aaaataatga aaagattatc tataaccgtc cgtttaacct
      661 tgctttttat attgctgctg tctgttgctg gcgctggaat tgtatggact ctctataatg
      721 gcctggcaag tgagttgaaa tggcgcgatg atacaacact cattaaccgg acagcgcaga
      781 tcaagcagtt gttaattgat ggggtaaatc cagatacgtt acctgtgtac tttaaccgga
      841 tgatggatgt tagtcaggat atcttgatca ttcatggtga tagcatcaac aaaattgtta
      901 accggacgaa tgtcagtgat gacatgttaa ataacatatc tgctactgag acaatcagcg
      961 cagctggcat ttacagaagc attattaatg atacagagat agatgcttta cgaattaata
     1021 ttgatgaagt ttcgccatca ttaacggtta ctgtggctaa attggcttca gccagacata
     1081 acatgcttga acagtataaa attaatagca ttataatttg cattgtcgcc attgtacttt
     1141 gctcagtatt aagtccgctg ttaatcagaa cgggattacg agagatcaaa aagttgagtg
     1201 gtgtaacgga agcgctgaat tataacgata gccgggagcc tgttgaggtt agcgcattac
     1261 cgagagaact aaaacctctt gggcaggcgt tgaataaaat gcatcatgct ttagtcaaag
     1321 attttgagcg tctaagtcag tttgctgacg atctcgctca tgaacttaga acgcccatta
     1381 atgcattact gggtcagaat caggttgcgc tcagtcaaac cagaagtatc gctgaatatc
     1441 aaaaaacaat tgccggtaac attgaagagc tggaaaatat ttcgcggtta acagagaaca
     1501 tactgtttct tgcccgggca gataaaaaca atgttttggt gaaactggac tcactttttc
     1561 tcaataagga agtcgaaaat ttgttggatt atcttgaata cctttcagac gagaaagaga
     1621 tttgctttaa ggtcgagtgc aatcagcaaa tctttgcgga taaaatttta ctgcaacgaa
     1681 tgttatcgaa tcttattgtt aatgccatta gatattcacc agaaaaatcg cgcattcata
     1741 taaccagttt tcttgatacc aacggctatc ttaatattga tatcgccagt cctggaacga
     1801 aaattaatga gcctgaaaaa ctcttccgta gattttggcg gggagataat tcgcgtcatt
     1861 ccgaaggtca gggacttggt ctttctttag tcaaagcgat tgccgaattg catgggggaa
     1921 gtgctacgta tcactatttt aataagcata atgtgttccg aattacgtta ccgcaaagaa
     1981 attaacatag ggcttcagta cgccataaat caaccatgaa aaaacgcagc cacgtcgtga
     2041 gtattaacgt ggctgcgatt gattatgcgc ttacattcat acgtaacagg gattaacccg
     2101 cataagctgc cagcatttcc tgcgccgcca gtttacccaa cgcattcgct gcgaaaggac
     2161 tgtcgccggt gagaacttta cggtccttat gtactcgccc ggtgatgtcg tcattaataa
     2221 tgttcatgcc cattttcttc agctcttcgc cgaagtacca ggtgagatga cccggcatat
     2281 agcctatctc tggcgtttgt ttgtctgcgg cgtctgggaa tgcgcaaatg gaataaccat
     2341 tcagtgggtt atcgccgtgg cgaagcgcca gaaaagccga cgggccgtgg caaagggaga
     2401 taacaaaacg gtcatttttg attgcccact gcaaagcagc agccacgtcc tggctttctg
     2461 gtaaaccaat aagtgcgcca tgaccaccag gaacaaagat tgctgcatat tcgctgtctg
     2521 cgttgaggct gacgacaaca tccgcgagtt ttttcggatt gcggaacaac gatttatgct
     2581 gctcaaagaa tggcattact ttttcatctt tgtgcggcat agcccagtat tcaaacttgg
     2641 tcatcagacc ggaaatggtc gccacttcga attcgaaacc tgcagcatgg agatgataca
     2701 acggcagcaa cgtttcaatc ggatggttac cggtcgagaa cagttttccg ttatcggtcg
     2761 gcaaataacg ttcgtccgcg gcgatcacca gaattttatg tttaccgcga tacggttttg
     2821 gatagtccac gccatcaaga tcagagacag gactggtata ttggctaagc gaatattctg
     2881 aagggaagaa tgcattatct tcagcaatat cgacctgcgg atttttactt gtttgaacag
     2941 tcatagtgta ttccttattg ttgcttagtt agggtagtca ctatatttgc gactgagctg
     3001 gtcgctattt ttaaccagtc tggaaaattg agtcaatata tcgaaagcgg tagtctgcgg
     3061 gtgtaagtat gtaatgatga tgctgcactg gtgttgaata ttattcacgc caaattttcc
     3121 ttattgagca acgtcgtcgc aagtggcctc aatgatttta gaccattatc agagatgcgg
     3181 ggcggaggga agagatttag tgctgtggaa tattgtggtg ctgatcaata tttatatccc
     3241 ggattggtag agaaaaagct tatttcatgc actatttttg caaatatttc tatatgggag
     3301 gcgtgtttgt tgcggattaa tacataaaga aatgtatttg gagtgttggc tggaagagta
     3361 gctggtagag atattgtgcc aggaggggca gggtaaggtg ctgtatcgat atgctttggc
     3421 aattgcgtgt gccgcagatg gagatgaaat gccaataaaa aagcctgagt tcgatgaggg
     3481 acgaactcag gctagtgtta tgttttacag taacacgccg cggcgcgttg gctgattaga
     3541 actggtagac gataccaaat gcgagttggt cgtcggtgtt cagtccatga gcgagaacat
     3601 aatcactctt atcaagcagg ttgatctgat aagcggtata tacgttcatg ttcttattga
     3661 agtaatacca ggtaccgatt tcaatgtagt tcagacgatc agaatctttg taacctgcta
     3721 cgtccagtgc tttagaatag gagtaaccga tggatggacg cagaccgaag tcgaactgat
     3781 attgcagaac agcttcgaag ttttgtgttt tgttcacaac atcaccgtcg tcggaattca
     3841 tgttgtgaga ttcaccgtac atcacagcag catagacgtt gttgggatca tattttgttg
     3901 acagtgccca aacttctgct ttttcacctt tactatcact agactggaaa tcagtacggt
     3961 cagagtttgc gtaagcacca gtaaatgaga aaccgtcaat agtgtaagtt acagataaac
     4021 catggccatc gccatttgct ttgtagttgc ctgtaccttc atttttaccc tgatattgca
     4081 gagcaaaatt tagaccttca accatgccaa agaaatctgt gttacgatag gttgcaacac
     4141 cgttggtacg gttggtcatg aacaggtcag taccagccca tgaatcaccg ccccactcaa
     4201 caaacatatc agtaaatgct tctgcatcat acgcgacacc gacattacga ccatagtcga
     4261 atgaaccaaa atctttgtaa ctcaaaccag cataagccaa acgagtttgg tcagggttgt
     4321 gtctgtttga tgcttccagg tctagttcaa actgaccgta accagtcaat tcagggttga
     4381 tctgagtttc acctttcacg cctacacggg cataagaggt atcttgagaa ttatcgcctt
     4441 tttcaccatt ttcacggtcg gtcagaatgc gctcaccaac catcttgccg tacagatcca
     4501 gtttattgcc atctttatta taaatttcag ccgcatttgc tgcgccagca actaataacg
     4561 ccgggaccag cattgccaga acttttcttt tcattatgta ttcccttgtg attataatct
     4621 tcatgaatat atcaataagt gccgttatcc
//
LOCUS       DAPHCV010000212         4587 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.214, whole genome shotgun sequence.
ACCESSION   DAPHCV010000212 DAPHCV010000000
VERSION     DAPHCV010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.214"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..585)
                     /locus_tag="RH033_004555"
     CDS             complement(<1..585)
                     /locus_tag="RH033_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDV2993631.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGF"
     gene            complement(700..775)
                     /locus_tag="RH033_004556"
     tRNA            complement(700..775)
                     /locus_tag="RH033_004556"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(740..742),aa:Thr,seq:ggt)
     gene            complement(782..856)
                     /locus_tag="RH033_004557"
     tRNA            complement(782..856)
                     /locus_tag="RH033_004557"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(821..823),aa:Gly,seq:tcc)
     gene            complement(973..1057)
                     /locus_tag="RH033_004558"
     tRNA            complement(973..1057)
                     /locus_tag="RH033_004558"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1021..1023),aa:Tyr,seq:gta)
     gene            complement(1066..1141)
                     /locus_tag="RH033_004559"
     tRNA            complement(1066..1141)
                     /locus_tag="RH033_004559"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1106..1108),aa:Thr,seq:tgt)
     gene            1503..2453
                     /gene="coaA"
                     /locus_tag="RH033_004560"
                     /gene_synonym="panK"
     CDS             1503..2453
                     /gene="coaA"
                     /locus_tag="RH033_004560"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDV2993632.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAINTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2482..3447)
                     /gene="birA"
                     /locus_tag="RH033_004561"
     CDS             complement(2482..3447)
                     /gene="birA"
                     /locus_tag="RH033_004561"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HDV2993633.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVTVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3444..4472)
                     /gene="murB"
                     /locus_tag="RH033_004562"
     CDS             complement(3444..4472)
                     /gene="murB"
                     /locus_tag="RH033_004562"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDV2993634.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKLVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1175 a         1155 c         1040 g         1217 t
ORIGIN      
        1 gaagccagcc agttccagga ttttcgcttc ccactctgcg tcgccttcca gcgctttcag
       61 agcagaacca cgaacgatcg gagtgtcgtc gcccgggaaa tcgtactgag acagaagttc
      121 acgaacttcc atttcaacca gttccagcag ctcttcgtca tcaaccatgt cacatttgtt
      181 caggaacacg atgatgtacg gaacgcctac ctgacgaccc agcaggatgt gctcacgagt
      241 ctgcggcatc gggccgtcag tcgcagcaac taccaggatc gcgccgtcca tctgagcagc
      301 accggtgatc atgtttttaa catagtcggc gtgccccggg cagtctacgt gtgcgtagtg
      361 acgggtcggg gtgtcgtatt caacgtgaga agtgttgatg gtgataccac gagctttttc
      421 ttccggcgcg ttatcgatct ggtcgaatgc gcgagcagca ccgccgtagg ttttagccag
      481 tacggtagtg attgcagcgg tcagcgttgt tttaccatgg tcaacgtggc cgatagtacc
      541 gacgttaacg tgcggttttg tacgttcaaa cttttcttta gacatcgatt gtccctctaa
      601 gacacggata aatcggtgat atcaccacat caaccaggca acatgcccga cttgttgaat
      661 gcaataaaca gaaggaaaaa acagggagga gaaaagaagt ggtgctgata ggcagattcg
      721 aactgccgac ctcaccctta ccaagggtgc gctctaccaa ctgagctata tcagcacatc
      781 ttggagcggg cagcgggaat cgaacccgca tcatcagctt ggaaggctga ggtaatagcc
      841 attatacgat gcccgcatcc tggaactcgg ctacctgatt ttcattctgc actgaatatc
      901 gagagaagct ctctttattc gagccggtaa gcgaacttat cgtctcgggc tacgccatcg
      961 cgtggccgaa attggtggtg ggggaaggat tcgaaccttc gaagtctgtg acggcagatt
     1021 tacagtctgc tccctttggc cgctcgggaa ccccacctga cttgatggtg ccgactaccg
     1081 gaatcgaact ggtgacctac tgattacaag tcagttgctc tacctactga gctaagtcgg
     1141 catcaagtag cgcgcattct atggagacat gcgagttcat gcaactaaaa aattgcataa
     1201 tttgttttat tggtcacatt ttatgcgacg cgatgaagaa acagccgaat ggagggcaga
     1261 ttagcacact ttttcaacat cattgtgctc aacaatgcgc tcctgctaaa ccataattct
     1321 ttttatcaga tggaatatct gtcacattgc ttttcaacga tagcttcctg ggagagattt
     1381 tttcttatta ttcctcccca tctggtgtta ccctcctgcc cattaaccca ttcaacagaa
     1441 ctgtggcgcg ccatggcaaa tatcgctttg ccgatagagc tatgaccgcc agaaacatgc
     1501 ttatgagtat aaaagagcaa acgttaatga cgccttacct acagtttgac cgcaaccagt
     1561 gggcagctct gcgtgattcc gtacctatga cgttatcgga agatgagatc gcccgtctca
     1621 aaggtattaa tgaagatctc tcgttagaag aagttgccga gatctattta cctctatcac
     1681 gtttgctgaa cttctatata agctcgaatc tgcgccgtca ggcagttctg gaacagtttc
     1741 ttggtaccaa cgggcaacgc attccttaca ttatcagtat tgctggtagt gtcgcggtgg
     1801 ggaaaagtac aaccgcccgt gtattgcagg cgctattaag ccgttggccg gaacatcgtc
     1861 gtgttgaact gatcactaca gatggcttcc ttcaccctaa tcaggttctg aaagaacgtg
     1921 gtctgatgaa gaagaaaggc ttcccggaat cgtatgatat gcatcgcttg gtgaagtttg
     1981 tttccgatct caaatccggc gtgccaaacg ttacagcacc tgtttactcg catcttattt
     2041 atgatgtgat cccggatgga gataaaacgg ttgttcagcc tgatatttta attcttgaag
     2101 ggttaaatgt cttacagagc gggatggatt atccacacga tccacatcat gtatttgttt
     2161 ctgattttgt cgatttttcg atatatgttg atgcaccgga agacttactt cagacgtggt
     2221 atatcaaccg tttcctgaaa ttccgcgaag gggcttttac agacccggat tcctattttc
     2281 ataactacgc gaaattaact aaagaagaag cgattaatac tgccatgacg ttgtggaaag
     2341 agatcaactg gctgaactta aagcaaaata ttctacctac tcgcgagcgc gccagtttaa
     2401 tcctaacgaa aagtgctaat catgcggtag aagaggtcag actacgcaaa taattttcag
     2461 gggagctaat gctcccctat cttatttttc cgcactacgc agggatattt caccgcccat
     2521 ccagggtttt attattccat cctgctcaag caataaagcg ccctgtttgt ctattccacg
     2581 tgaaatgcca aatatttctt tatcaccaat gataagtttc actgggcgat taataaaatt
     2641 atccagcttt tcccagcgcg aaagataagg tgccaatcct tcttgctcga agagttccag
     2701 cgccgcgcgt aattcacgta tgagcatggc cgccaacgta ttacgatcga gattgattcc
     2761 cgcttcctgc agcgtgatcc acccttgatt aacgacactc tcttcaacac gacgcattgc
     2821 catgttgatc ccggctccaa tgactatttg cgccgcatcg ccggttttgc cagttagctc
     2881 cacaagaatg cctgccagct tgcgatcctg cagatagagg tcattaggcc atttaacacg
     2941 aactttatct gcacccagct tgcgtaatac ttccgccatc acgataccga taaccagact
     3001 taaaccaatc gccgccgccg ggccttgttc cagacgccag aacatcgaca aatataagtt
     3061 tgcgccaaaa ggcgaaaacc atttccgccc ccggcgacca cggccagcct gctggtattc
     3121 tgcaacacag gcatcgcccg atttaagctc tccgatacga tcaagaaggt actgattcgt
     3181 ggagtcaata acgggcagca cagttacact accgccatcc agctgaccca atatctgttc
     3241 agcattaagt aactggatag gctcaggcag gctgtatcct ttacccggaa cggtaaagac
     3301 atcaacgccc cagtcacgca gtgtctgaat gtgtttatta atagccgccc ggctcattcc
     3361 taacgtttca cccaactgct caccagagtg aaattcgcca ttcgctaaca gcgcgattaa
     3421 tttcagcgga acggtattat ccttcatgaa atcgtctcca ctgcgctaac ttcacctgat
     3481 gcaccaataa agcgaacttc aggctcaagc cagacattaa atttttcacc cactttttgt
     3541 cttacatgat gtgccagttg caccacatct tcgctttttg cattgtcttc attaatgagg
     3601 actaacgcct gttgacggtg caccgcagcc ccacccattt gcatcccttt tagctggcac
     3661 tgatcgataa gccaacctgc tgccagtttt actgaaccat ccgcctgggg ataatttggt
     3721 gctgttggaa attgtgccag taatgcttta gccgtttcgg cagatacaac agggtttttg
     3781 aagaaactac cggcattgcc attcactttt ggatcaggga gtttggtggt gcgcatatga
     3841 cacaccgcat taaatacttg ctgtggcgtt acggttgtag gatccagacg agttaagtca
     3901 ccatacgtta gtacaggttg ccactctttt ggcagacgca gacctacggc tacaatggcg
     3961 aagcggtcct ggtattcatg tttaaaaata ctgtcgcgat agccaaaacg gcactctttg
     4021 gcagttaagc gcactagctt gcctgtcgcc agttcaacac agtcaacata agcgcaaact
     4081 cgctgtaatt ctacgccata agcaccaata ttctggatag gtgatgagcc gacacaacca
     4141 ggaattaatg ccagattttc cagaccaggc ataccttcct gcaaagtgta ttttaccagg
     4201 cgatgccagt tttctccggc tcctacatgt aaataccacg catcaggttc atcatgaatt
     4261 tcgatacctt taatccggtt gatgatcacc gtgccgcgat agtcttccag aaaaagtaca
     4321 ttacttcctt cacccagaat aagaacgggt tgtccttctg cggttgcatg ctgccaggca
     4381 ttgagtagtt gttgttcgtc ttcggcacat acaatgtgct gagcattatg atcaatgcca
     4441 aatgtgttcc agggttttaa ggagtggttc atagctgctt tcctgatgca aaaacgaggc
     4501 tagtttaccg tatctgcggg gggatggctt gtagatatga tgacaggaag agtttgtaga
     4561 aacgcaaaaa ggcccagtct tccgacc
//
LOCUS       DAPHCV010000213         4584 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.215, whole genome shotgun sequence.
ACCESSION   DAPHCV010000213 DAPHCV010000000
VERSION     DAPHCV010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.215"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(240..653)
                     /locus_tag="RH033_004563"
                     /pseudo
     CDS             complement(240..653)
                     /locus_tag="RH033_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015377870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
     gene            651..908
                     /locus_tag="RH033_004564"
                     /pseudo
     CDS             651..908
                     /locus_tag="RH033_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(1142..1999)
                     /gene="sitD"
                     /locus_tag="RH033_004565"
     CDS             complement(1142..1999)
                     /gene="sitD"
                     /locus_tag="RH033_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HDV2993635.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKSWALMG
                     DAMSHAVFPGIVLAWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAVKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQASGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISILVSMTGVWLSFFIDSAPAPTIVVLFAVMFIMTFTVTSINARTKENA
                     EPRDLLSSD"
     gene            complement(1996..2853)
                     /gene="sitC"
                     /locus_tag="RH033_004566"
     CDS             complement(1996..2853)
                     /gene="sitC"
                     /locus_tag="RH033_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HDV2993636.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQLTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLMIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2850..3677)
                     /gene="sitB"
                     /locus_tag="RH033_004567"
     CDS             complement(2850..3677)
                     /gene="sitB"
                     /locus_tag="RH033_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HDV2993637.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPGGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWPFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREER"
     gene            complement(3677..4582)
                     /gene="sitA"
                     /locus_tag="RH033_004568"
     CDS             complement(3677..4582)
                     /gene="sitA"
                     /locus_tag="RH033_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HDV2993638.1"
                     /translation="MKKVTMLLGCLVLTCSIAFQASAAEKFKVITTFTIIADMAKNVA
                     GDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNGVPE
                     VIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDAFIKYDPANAQTYQRNA
                     DTYKAKITQTLAPLRKQIAELPENQRWMVTSEGAFSYLARDLGLKELYLWPINADQQG
                     TPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTENGPV
                     PTYIDLLKVTTSTLVQGIKAGKREK"
BASE COUNT         1238 a         1185 c         1073 g         1088 t
ORIGIN      
        1 gaaggttcta tttattgaca ttaataccct cgacaattgc tgagcagaag cccccacgac
       61 aagtgacaga atgacaacat ctacagtggg gagcgtttta tcgatgaaca agcaaccaag
      121 aagtaacacg gcataggtga cgacaatgcc agtaagcata gaaataacaa aaacgtgagc
      181 tttcatcatt cctccagaat aaccccgacg tgtaagcctg ggggattggc acaataatgt
      241 catttaaagg cagactttcc ggcaaattgc gccgatgaac ccagttcatc ctcaattctc
      301 ataagctggt tatatttttc aatacgctca ccccggcatg gagctcccgt tttgagatga
      361 cctgcgcgca ttgcaactgt catatcggcg ataaagctgt ccactgtctc accactgcgg
      421 tgtgagataa aggtgcccca gttgttgtca tggcaaagtt gtaccgcatc aaaggtttca
      481 ctcagggaac caatctggtt gagtttaatc aatgctgaat tggcaaggtt ttcgtcaatg
      541 ccgcgctgaa tatactttac gttggtcaca aacagatcgt cgccaaccaa ttctatctgc
      601 tctcccaatg caccatgcag gattttccat ccagcccagt catcttcagc taccgttaag
      661 ggcaggaaga tcaactaggt gctctggggc tggtgaccaa tgcggttgtg ctgtgaaaca
      721 cactttacat gcaggaggcg ttgtcacatc ttcgcaaatc gggagaaact cctgaggagt
      781 agcatccggc aagattatcg ctgctgatac atagccatat aaatatgctg gggcattata
      841 cgtcttcgtt accggaagat attcttaagg gtgagttaag atcattaaac ttcaatacaa
      901 acaattaatt aaactcttag cgtggttttt tacatcactg gccctcgccc cccaattttc
      961 tctatgtttt gccactactt ccctcatttt actgcgctgt ttgctacccc tgagaatcga
     1021 cattacttgc tatccagtcc gcagcaactc tctggcaaca aaacttatca gaatcataag
     1081 tctgtgcatt attcgttcaa gcgcatcgcc aataagcgga tgttgctggc cgagtgttct
     1141 gctaatcgga tgataacaaa tcccgtggtt cggcattttc ctttgtgcgt gcgttgatgc
     1201 tggtaacggt aaacgtcatg atgaacatga ccgcgaataa gacaacaatt gtcggagctg
     1261 gcgcgctgtc gataaaaaag gacagccaga caccagtcat tgagaccaat atcgatatgc
     1321 cagtcgccag caggagcgca atatgaaagc gttgtgtcag gagtaccgca atggcaccag
     1381 gcgcaatgag aagagaaata gagagaatga tacctaccgc tttcagcgtc gccacaatgg
     1441 tcagggagac catgcacagc agcccataat gcagccatct cgtatgcaaa ccgcttgcct
     1501 gcgcctgttg ataatcaaag ctgaacagca aaaaatcgcg ccattttacg ctaataacaa
     1561 gtgtaaccag tccggcaata atcaccgtct ggattatatc gccgatggtt atcccgagca
     1621 tgtcgccgaa aagaatatga tcaagatgta cgtctggttt gactgcaata tacaagatca
     1681 gacctgcggc aaacatgcca gaaaaaacaa tccccatcac tgtatcttgc ttgattcggc
     1741 tgttgtcttt caggtatccc gtcgctaccg cacagaatac tccggcgacg aatgcccctg
     1801 tagccagagg taaccccaga atccaggcaa gcacgatgcc aggaaaaacc gcatgactca
     1861 tcgcgtcacc catcagtgcc caacttttca ggaccagaaa gaccgacagc agcgcacagg
     1921 gtatggaaac aaccagtgaa atcagcagtg cgtgactcat aaaggtgaac tgcagtggct
     1981 ccagtaataa tgccatcatg agcgatcctc cagcgcttta tgcgcacgcc gacgatttgc
     2041 caaaagccca tgggtgggcg caaagacaaa tgctaaaaga aacagcagtg tctgcgccac
     2101 cacgatgatt ccacctgtcg cgccgtccag aaagtaactg acccacgcgc cgaggaaact
     2161 ggtcacactg ccaatagtta cagcaatcat gagcaggcgg ggaaaacgat cggttaacaa
     2221 ccatgctgtt gcaccgggag tcaccaccag acagatcacc agaaaggcac caaccgtttg
     2281 cagcgccgca acggtcgaaa ccgacagcag ggtgaagaag aggattttta atctgcccgg
     2341 atgcaatcca atagcacggg cgtgattctc gtcgaaaaag gtcaccataa gatctttcca
     2401 tttgaaaaaa agcacgatga tcgagagaat gccaatgata gtgagttgca ggatatccgc
     2461 cggatcgatt gccagaatat tgcccaggac gatggtctga atgttcacgg atgtcgggtt
     2521 caatgacacc ataaacagcc ccaggccaaa aaaggacgag aagatcaggc cgatgatggc
     2581 atcttctttt aagcgagtga gttggttaag aaacagcatg ctgcccgctg ccagtccgcc
     2641 ggaaaagaag gctcccagag aaaacggaag ccccagcata tatgcccccg caacacccgg
     2701 gacaatagag tgcgacagcg catcgccaat cagcgaccag ccttttagca tcagatagca
     2761 tgacaggaaa gcgcagagac cgccgaccat cgccgagacc cacatcgcat tgagcatata
     2821 ttcatagctg aagggttcca gtagcacgtt catctttctt ccctctgcac cgctgacggt
     2881 cgatgtgcca caaaagggcg ttcatcgtca gtaataatgc tttcttccga gccgttaagt
     2941 gtgacatggc gcagtacgcc gctaaaagcc agctccaggt tctcggctgt aaaagtggtg
     3001 tctgtcggcc cgcttgccag gacggtgcct ttgaccatga ccgtataatc gcaaaatgtc
     3061 gtgacggatc cgagattgtg cgttgagaca agcatcgttt tgccttccgc gcgtaactcc
     3121 ctcaacaggc tgatgatttt cgcttcggtt ttcacatcaa cgccggtaaa aggttcatcc
     3181 agcaggatca catcaccctg ctgtgcaatc gctctcgcca gaaagacgcg tttcttttgt
     3241 ccaccggaaa gctcgccgat ttgtcgatgg cgaaaatcca ccatatcgac acgttccagc
     3301 gcatcggtga caatctgacg atccctcttt ttggcgatac gcaacatacc catatgccca
     3361 tagcggccca tcatcaccac atcttccacc agtacaggaa atggccagtc aacctcttct
     3421 gactgaggga cgtaggcaac caggtttttc tgtagcgcct gtcgtgtggg aatacccaga
     3481 acagatatct tcccgctggt cagacgcaca aatcccataa tcgctttaaa caacgtcgat
     3541 tttccggaac cgtttacccc aaccagagcg gcaatagatc cgcctggtac tgtgaaggat
     3601 gcatcgcgca gtgctgtgtg cccgttacgc caggtgacag taacatcatt aacgacaatg
     3661 cctgcagatt gcatcattat ttctcccttt ttccggcttt aattccctgt accagcgtac
     3721 tggtagtaac cttcagaagg tcgatatacg taggtaccgg gccgttttct gtgctcaggg
     3781 aatcgacata gagcacacca ccgtagtgcg cgccggtttc acgcgcaacc tgacgcgctg
     3841 gtttatcgga aatcgtactc tcgctaaaga ctgccgggat atgatttttc ttaactatat
     3901 caacaacctt acgtacctgc tgcggtgttc cttgttgatc ggcattaatc ggccacagat
     3961 aaagctcttt tagccccaaa tcgcgtgcga gataagaaaa agccccttca ctggtgacca
     4021 tccatcgctg attctcaggg agttccgcaa tctgcttacg caggggggca agggtttggg
     4081 taatcttggc tttataagta tcggcattac gttggtaggt ttgtgcattt gccggatcgt
     4141 attttataaa cgcatcacga atattatcga cgtaaatcag agcattatct ggcgacatcc
     4201 aggcatgggg gttaggtttg ccctcatagg gcccttcggt gatccctact ggcgtcacac
     4261 ccgaagagac aattacttct ggaaccccat tgagatgctg gtaaaagcgt tggaaccaca
     4321 attccagatt cataccattg gcgagaatca gttgtgcccc ctgcgcacgt ttaatatcgc
     4381 caggggtagg ctgatactca tgaatttctg caccaggctt ggttatggat gagacttctg
     4441 cagcatctcc agccacgttt ttggccatat ctgcgatgat ggtaaaagtt gtaatgacct
     4501 tgaatttttc agctgcactc gcctgaaatg ctatcgagca ggtgaggact aggcacccca
     4561 agagcatggt tacttttttt attg
//
LOCUS       DAPHCV010000214         4567 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.216, whole genome shotgun sequence.
ACCESSION   DAPHCV010000214 DAPHCV010000000
VERSION     DAPHCV010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.216"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            239..1396
                     /gene="acrE"
                     /locus_tag="RH033_004569"
     CDS             239..1396
                     /gene="acrE"
                     /locus_tag="RH033_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HDV2993639.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     SYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENAASNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVIPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNNKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1408..4512
                     /gene="acrF"
                     /locus_tag="RH033_004570"
     CDS             1408..4512
                     /gene="acrF"
                     /locus_tag="RH033_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HDV2993640.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERSGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTAHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASQLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
BASE COUNT         1110 a         1103 c         1201 g         1153 t
ORIGIN      
        1 ctatacatat ttcaatttac gagattttaa ttctacctct ttcattccgc gtcaaaacaa
       61 aacagtagaa tattaatctt tttttgtgtt tatgtgcctt gagttgccag tattcataac
      121 tattccttac atcgacgaat gataatttgt aggatagcga actgtatttt tctttctgcg
      181 agttaacgca ttgccttttt gggcaaataa cgcgcttttg gttttttgag gaatagtaat
      241 gacgaaacat gccaggtttt tcctcctgcc ctcctttatt ctgatctccg cggctttaat
      301 cgccggttgt aacgataagg gagaagagaa agctcacgtc ggtgaaccgc aggttaccgt
      361 tcatattgtg aaaacggccc cgttagaagt taagactgaa ttaccaggcc gcaccaatgc
      421 ttaccgtata gccgaagttc gcccacaggt tagcgggatc gtactgaatc gcaatttcac
      481 tgaaggcagc gatgtgcaag caggccagtc cctgtaccag atcgatcccg cgacctatca
      541 ggcaagttat gacagcgcga aaggcgaact ggcgaaaagt gaagccgccg ccgccatcgc
      601 gcatttgacg gtaaaacgtt acgttccgct cgtgggtacg aaatacatca gtcagcagga
      661 gtacgaccag gccattgctg atgctcgcca ggccgatgcc gccgtgattg ccgcaaaagc
      721 cacagtcgaa agcgctcgca tcaatctcgc ttataccaaa gtgactgcgc caattagcgg
      781 acgtatcggc aaatcgactg tgaccgaagg cgctcttgtc actaatgggc aaacgaccga
      841 actggcgact gtccagcagc tcgatcctat ctacgttgat gtgacccaat ccagcaacga
      901 ttttatgagg ctgaagcaat ccgtagagca aggaaatctg cataaggaaa acgccgccag
      961 caacgtagag ttggtcatgg agaacggtca aacctatccc ctgaaaggta cgctgcaatt
     1021 ctccgatgtt accgttgatg aaagcaccgg ctccataacc ctacgtgccg tcatccctaa
     1081 cccgcaacat acgcttttgc cgggtatgtt tgtgcgcgca cggattgatg aaggcgtcca
     1141 acctgacgcc attcttatcc cgcaacaagg cgttagccgc acaccacgtg gtgatgcaac
     1201 cgtgctgatt gttaacaata aaagccaggt tgaagcgcgc cctgtcgttg ccagtcaggc
     1261 gattggcgat aaatggttga ttagcgaagg tctgaaatct ggcgatcaag tcattgtcag
     1321 cggcctgcaa aaagcgcgtc cgggagagca ggttaaagcc actaccgata cccccgcaga
     1381 tactgcatcg aagtaaggca atctgacatg gcaaactttt ttattcgacg accgatattt
     1441 gcatgggtgc tggccattat tctgatgatg gcgggcgcac tggcgatcct acaattgccc
     1501 gtcgcccagt atccaaccat tgcgccgcct gcggtttctg tttcggcaaa ctatccgggt
     1561 gctgatgcgc agaccgtgca ggatacggtg acgcaggtta tcgaacagaa tatgaacggt
     1621 atcgataacc tgatgtatat gtcctccacc agcgattccg ccggtagcgt gacaattacc
     1681 cttactttcc agtccgggac cgatcctgat atcgcgcaag tgcaggtgca gaacaaactc
     1741 cagctcgcca cgccgttgct gccgcaggag gttcagcagc aggggatcag tgttgaaaag
     1801 tccagtagca gctatttgat ggtggcgggc tttgtctctg ataacccagg caccacacag
     1861 gacgatatct cggactatgt ggcctctaac gttaaagata cgcttagccg tctgaatggc
     1921 gtcggtgacg tacagctttt cggcgcacag tatgcgatgc gtatctggtt ggacgccgat
     1981 ctgctaaaca aatataaact gacgccggtt gatgtgatta accagttgaa ggtacagaac
     2041 gatcagatcg ctgccggaca gttgggcgga acgccagcgt taccagggca acagttgaac
     2101 gcctcgatta ttgctcagac gcggttaaaa aatccggaag aattcggcaa agtgaccctg
     2161 cgcgtaaaca gtgacggctc ggtggtgcgt ctgaaagatg tcgcacgggt tgaacttggc
     2221 ggtgaaaact ataacgttat cgctcgcatc aacggaaaac cggcggcggg cctgggtatt
     2281 aagctggcaa ccggcgcaaa tgctctcgat accgcgaaag ccattaaggc aaaactggcg
     2341 gaattacagc cattcttccc gcagggaatg aaggttctct acccttatga caccacgcca
     2401 ttcgtccagc tttctattca cgaagtggta aaaacgctgt tcgaagctat tatgctggtg
     2461 ttcctggtga tgtatctgtt cttgcagaat atgcgagcaa cgctgatccc caccattgcg
     2521 gtacccgtgg tgttgttagg gacgtttgcc atcctcgccg cttttggtta ctccatcaac
     2581 acactaacga tgttcgggat ggtgcttgcc atcgggctgc tcgtcgatga tgcgatagtg
     2641 gtggtggaga acgtcgaacg cgtgatgatg gaggataagc tcccgccaaa agaagcgacg
     2701 gaaaaatcga tgtcgcaaat ccagggcgcg ctggtaggta tcgcgatggt actgtcggcg
     2761 gtatttattc cgatggcatt cttcggtggc tcaacagggg caatttatcg acagttctct
     2821 atcactattg tttcagcaat ggcgctttca gtactggttg cgctgattct cacccccgcg
     2881 ttatgtgcaa cattgctgaa accagtctct gccgagcatc acgaaaataa gggaggattc
     2941 ttcggctggt ttaacaccac tttcgatcat agcgttaatc actacaccaa tagcgttggc
     3001 aaaatcctcg gttccacagg gcgatattta ctgatctatg cgctgattgt tgcaggaatg
     3061 gtggtgttgt ttttacgtct tccgtcttcc ttcttacctg aagaggatca gggtgtcttt
     3121 ctgaccatga ttcagttacc cgctggcgcg acgcaagagc ggacgcaaaa agtgttggat
     3181 caagtgacgg attactatct gaagaacgag aaagcgaacg ttgaaagtgt ctttacagtt
     3241 aacggcttta gcttcagcgg ccaggcacaa aacgccggta tggctttcgt cagtctgaaa
     3301 ccgtgggaag agcgtagtgg tgacgaaaac agtgcggaag cggtaatcca tcgtgccaaa
     3361 atggaattgg gcaagatccg cgacggtttt gtcattccat tcaatatgcc agccattgtt
     3421 gaactgggca cggcaacggg tttcgacttt gagttaattg atcaggctgg gctgggtcat
     3481 gatgccctga cccaggcccg taaccagttg cttggcatgg cggcgcaaca tcctgccagc
     3541 ttagtcagcg tgcgtcctaa tggtctggaa gacaccgcgc agtttaaact ggaagttgac
     3601 caggaaaagg cgcaggcatt aggtgtttca ctttctgaca tcaatcagac catttcaaca
     3661 gcgttgggtg ggacttacgt taacgacttc attgaccgtg gtcgcgtaaa aaaggtgtat
     3721 gttcaggcgg atgccaaatt tcgtatgctg ccagaagatg tcgataaact ttatgtccgt
     3781 agcgccaatg gcgaaatggt gccattctct gcctttacca ctgcacattg ggtgtatggt
     3841 tctccgcgac tggagcgcta caacggtctg ccgtcaatgg agattcaggg ggaagccgcg
     3901 ccaggaacca gttccggcga tgccatggcg ttgatggaaa accttgcatc acaattacca
     3961 gctggtattg gttatgactg gacgggtatg tcatatcagg aacgcttatc gggaaaccag
     4021 gctcccgctc tggtagcaat ttcctttgtg gttgttttcc tgtgccttgc tgcactctat
     4081 gaaagctggt caattcctgt ctcggttatg ttggtcgtgc cgttagggat tgtcggcgtg
     4141 ctactggcgg cgacactctt taatcaaaaa aatgacgtct actttatggt gggcttgcta
     4201 acgacaattg gcttgtcggc taaaaacgct atattgattg ttgagttcgc taaagatctc
     4261 atggagaaag agggtaaagg tgttgttgaa gcgacactaa tggcagtacg tatgcgtctg
     4321 cgtcctatcc tgatgacctc tctcgccttt attctcggcg tattaccgct agctatcagt
     4381 aacggtgccg gcagtggcgc gcagaacgca gtgggtatcg gggtaatggg cgggatggtt
     4441 tctgcaacgt tgctggcaat cttcttcgtg ccggtgttct ttgtggtgat ccgccgttgc
     4501 tttaaaggat aaatcagaaa cataaaggca ctttcgggtg cctttattat ttccagtgaa
     4561 acccata
//
LOCUS       DAPHCV010000215         4499 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.217, whole genome shotgun sequence.
ACCESSION   DAPHCV010000215 DAPHCV010000000
VERSION     DAPHCV010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.217"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..567)
                     /locus_tag="RH033_004571"
     CDS             complement(<1..567)
                     /locus_tag="RH033_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2993641.1"
                     /translation="MNKYIIPWCFIVFIFSFSGATFAAPKGICTSDNGSFHSTLDFSG
                     YLITADENRVGKTFNKTVTNGDSYPAHCHCDTGNVGEFPYIYYTARINESLGYAGVRS
                     NVNYFNLNPNLDVGISIDLLGVGYINAPFEYHANRPTGGSYKCSRTDPLSISSGAKAI
                     IYFYIKKTFAGKLIIPETLVAKLYGTISR"
     gene            complement(578..3100)
                     /locus_tag="RH033_004572"
     CDS             complement(578..3100)
                     /locus_tag="RH033_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV2993642.1"
                     /translation="MMTTRIKVGLTAGTCLIFSQSLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINSKMFERQDVTFVQDNPDADLHACFVAIKKTLTTFGVK
                     VDALKSLNDVDETVCIDPGPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPSRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTTDGS
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPVSQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQVQILHGFPYGFTLYGGMQA
                     AEKYGSAALGVGKELGALGAISFDVTHARAKFSHADTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNNPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGTYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTRVGYRVLFRVAGTKGKPAPFGAIATVQNTSSADSGIVGDQGELYLSG
                     LPEKGQVMLSWGENAATTCTFDYSLSIPESESGLIEQGVTCH"
     gene            complement(3125..3856)
                     /locus_tag="RH033_004573"
     CDS             complement(3125..3856)
                     /locus_tag="RH033_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDV2993643.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLVQPALTYAGVVIGGT
                     RVVYLSNNPDKSISVFSKEEKIPYLIQAWVDPFNKEDKIKAPFTVIPPVSRLEPAQEK
                     VLRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKAANSLEIAVKTRIKLFWRPASIRL
                     IPEDAAPKVKWHREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMIWRFINDYGAVSDPIKMTL"
     gene            complement(3904..4476)
                     /locus_tag="RH033_004574"
     CDS             complement(3904..4476)
                     /locus_tag="RH033_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2993644.1"
                     /translation="MKRNIIGGAFTLASLMLAGNALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQQFSIKLEKCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT         1217 a         1079 c          977 g         1226 t
ORIGIN      
        1 acggcttatg gttccgtaca attttgccac gagagtttca ggaataatta actttccagc
       61 aaaagtcttc ttaatataaa aataaattat tgcttttgca ccactggaaa tacttaatgg
      121 gtccgtgcga ctacatttat atgaacctcc agtaggcctg ttagcatggt attcaaaagg
      181 tgcattaatg tatcctacac cgagaaggtc tatcgatatt ccaacatcca gattaggatt
      241 caaattaaag tagtttacat tagaacgaac acctgcataa cctaaggact cgtttattct
      301 cgctgtataa taaatataag gaaactctcc cacgttccct gtatcacaat ggcagtgagc
      361 aggataagag tccccatttg tcacggtttt attaaaggtc tttcccactc tgttctcgtc
      421 tgccgtaatc agatagccgg aaaaatcaag tgtgctatga aaggacccat tatctgaagt
      481 gcagatacct tttggggccg caaatgttgc gccactaaag gagaagataa aaacaataaa
      541 gcaccatggt ataatatact tgttcataaa aatcctttta atgacatgtc acaccttgtt
      601 caattaagcc gctttcactt tctggtattg aaagtgaata atcgaaggtg catgtagtgg
      661 cggcattttc tccccaggag agcatcacct gccctttttc aggaaggccc gagagataaa
      721 gctccccctg gtcaccgaca attcctgaat cagcggagct tgtattttgt actgtagcaa
      781 tagcgccaaa aggtgctggt ttaccttttg tacccgcaac cctgaaaagc acacgataac
      841 caacacgagt aacaaattcg gccttaacca ctgcaccacg ggtgggaacc atcgtaacga
      901 cggtattatc aagttctata ttcgacttcg agaaatagtt atcgctgatt gctacccggt
      961 tttcatcata agggtttagc tgtgtcttga ccgtatagcc gcgccagtcc gtcgcagcac
     1021 cgcgcatatt gtctatttct aacccggcag cccctggtgc tttaacaaga gctatggttt
     1081 cacccaactc ctggcttaac gttacgcctt cgctgtgcgc cagaattccc cctctgatac
     1141 cataattcag cctctgagaa ttttggctgt agttatagct gccgttcacc gtgccataag
     1201 tgccatcata ggtcagggac atattaccgc caacctcatc atcatggctg ttataggact
     1261 gcattaaatt ccaggaaagg ttatttcttt ccagcgccgt accgctgagt ccaagttgat
     1321 gtgagctacc ggaattatcg ttttgtgaaa cagaataggt ggcgtaacta tttccccacc
     1381 agcccgataa aggaacagat aaagagagca tgtagatctg ctcactggtg ttatcactgg
     1441 catggttgtt ccccgtcccc tgccgggcag tattggaata actccaggag acgttccacg
     1501 agagacgctt ccagctactg ctgtagccaa attgcattaa tcgttcaaca tcatcggtat
     1561 gccagtactg ctggcgactt aatgtcagat ataagttgcc atagtctctg cccagtgatt
     1621 gcgttaacgt tccttccaca cggctgcggc ggttaccggt ttcccagaaa tcttcaggat
     1681 tattacgccg cgacgcccat tcattgagag tatagtaacc ttcggtggag taacgatagc
     1741 caaccaggcg caagctggta tccgtatcgt caaagcgttt cgaatacaga aagcgataag
     1801 actgaccggt ttccgtatca gcatggctaa atttcgcacg ggcatgtgtc acatcgaaag
     1861 aaattgcacc cagtgcgcca agctctttac cgacacccaa agcggcagaa ccatactttt
     1921 cagcagcctg catacctcca tacaaggtga aaccgtaggg aaaaccatga aggatctgca
     1981 cctgaagaac gtcaggggta aagccatctt catcgcgtaa ttctcccaca ctgacatcaa
     2041 catcagtttg accttcacgt ttgaggatgg ctaacgacgc ataaggttga gtaaatcgtt
     2101 gttcgctacc gtcttcttct ttgatggtta cgtccagatc accacctgta ctggcggtgt
     2161 tgaggtcggt aatttcaaaa gcaccttgtg gaacggctga ctgataaatc acgtagccat
     2221 tctggcgaat aatgacagtg gcgttagact ttgcaatccc acgcactacg ggggcaaatc
     2281 cgttttggct gacaggcaac atatcgttat cggtatacaa gcgtgcgcca cgaatttgcg
     2341 tactgtcgaa gatatcgctc gccgtccacg tatcacctat cataatctgg cttcttaacg
     2401 cagctatatc acgttgtatc caggtgctta tagagttgta ttctgcactg ccatcggtcg
     2461 tatttaaggt gctgtaattg cgtaatcgcc acggcccaat attcagtcca ttgcgcagat
     2521 tgagataact ggtatctgtc tcttgtgaac cataatccga acgtagagta tgtgaaccag
     2581 aaaaatcgta gttgatggtg agcgcgttaa tcccctcatc ccaacgcgag gggctgatgt
     2641 aatcataagc catcgcatcc atatagattt ggggaatgga gatattcagt tgcagtttat
     2701 cgctgtcgaa ctgccatgat gagccttcaa tgcgtggccc aggatcaata cagactgttt
     2761 catctacatc attgagcgat ttcagcgcat caacttttac gccaaaggtg gttagcgttt
     2821 ttttaatagc gacaaagcag gcgtgtaaat cagcatcggg attatcctga acaaaggtca
     2881 catcctgacg ttcaaacatt ttactgttga taaaaatatc aacgctataa acacctgggg
     2941 gcatcaggtt tgcccgatta aactggcgaa tatcgactcc ggattgatgg tcgatttcca
     3001 gaagcgccgg attgaataca ctgacctcgg ccatcaggct ttgcgagaaa atcagacacg
     3061 tccctgccgt gaggccaacc tttattctgg tcgtcatcat aatacgccta tccttttccc
     3121 ttgcttacag cgtcattttt atcgggtcac tgactgcgcc gtaatcatta ataaatcgcc
     3181 agatcatttg gcctcccgca gaattagcag gtaaaggtaa cgtgagcgtt tcaaaaggac
     3241 ggatcatgtt gaggggcaca tcatgaccat caacgatgac atccatcaca ctgatatgaa
     3301 tcgggttggg attctctgca atcaggtttc gtccttcccg gtgccacttc accttcggtg
     3361 cagcatcttc gggaatcagg cgaatgcttg ccggtcgcca gaacagcttg atacgcgttt
     3421 taactgcgat ttccagtgag ttcgccgctt tgttgctcgc agagggcgga atatttttaa
     3481 tattcagcca gaagactgac tcgcgatcat caggcaatga cacacctttg gtatgaataa
     3541 tgcgtaatac tttttcttgc gcgggttcta atcgtgatac cggaggaata acggtaaacg
     3601 gtgctttgat tttatcctct ttattaaacg gatctaccca tgcctggatg agataaggaa
     3661 ttttttcttc cttgctaaat acagaaatag atttatctgg gttattactt aagtaaacta
     3721 cccgtgtacc accaatgaca acgcctgcat aggttaatgc tggttggact aaaaaaacac
     3781 tgagtataat cgcggtaaat aacttacatg aaacggttgt tttaaagagc ctgattaaac
     3841 aactttttct ggacataacg actccctggc caaagccaac tgaataaaaa ggcccttgat
     3901 aaattatttc ttatattcga cagtaaattg tgaatcggcg ttcgccgtac ccgccgttac
     3961 ggttgcattg gttgccacat agcgtgcaat aaacttcatt tcagccttgc cttcagcgtc
     4021 gatagcggta taggcagaat cgttataaag tttaacctgc gagttatctg actggttaaa
     4081 aatacgaata ccgacacccg ttgccgcaat cgcttgtcct gtaccggtaa aatcgccagg
     4141 gttgcccgct gtaagcggcg tacccacttt caggtcgccg tcgccgccag gcgcttctgt
     4201 accatcaaaa cgaacggctg cctgagtgta ggttgccggg cacttttcaa gcttaatact
     4261 gaactgctgt ggtgacgcca gactacctac acctgaaaaa gcatttttac tgactttacc
     4321 cagtgggaca atttgatcgg ctgtatcgga ggtaacttca caagtcgtgt cgacaatttc
     4381 accgacgaag tgaacaacac catcttctgc cagtgcatta ccggccagca ttagagatgc
     4441 cagagtgaat gcaccgccta taatattacg cttcataaaa actccattga tataatata
//
LOCUS       DAPHCV010000216         4479 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.218, whole genome shotgun sequence.
ACCESSION   DAPHCV010000216 DAPHCV010000000
VERSION     DAPHCV010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.218"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            265..1410
                     /locus_tag="RH033_004575"
     CDS             265..1410
                     /locus_tag="RH033_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C beta-lactamase CMY-2"
                     /protein_id="HDV2993645.1"
                     /translation="MMKKSLCCALLLTASFSTFAAAKTEQQIADIVNRTITPLMQEQA
                     IPGMAVAVIYQGKPYYFTWGKADIANNHPVTQQTLFELGSVSKTFNGVLGGDAIARGE
                     IKLSDPVTKYWPELTGKQWQGIRLLHLATYTAGGLPLQIPDDVRDKAALLHFYQNWQP
                     QWTPGAKRLYANSSIGLFGALAVKPSGMSYEEAMTRRVLQPLKLAHTWITVPQNEQKD
                     YAWGYREGKPVHVSPGQLDAEAYGVKSSVIDMARWVQANMDASHVQEKTLQQGIALAQ
                     SRYWRIGDMYQGLGWEMLNWPLKADSIINGSDSKVALAALPAVEVNPPAPAVKASWVH
                     KTGSTGGFGSYVAFVPEKNLGIVMLANKSYPNPVRVEAAWRILEKLQ"
     gene            1504..2037
                     /locus_tag="RH033_004576"
     CDS             1504..2037
                     /locus_tag="RH033_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HDV2993646.1"
                     /translation="MRILPVVAAVTAAFLVVACSSPTPPKGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLDKVTATYSLRDDGGINVINKGYNPDREMWQKTEGKAYFTGDPSRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEMKQQMLAIAT
                     REGFEVNKLIWVKQPGA"
     gene            complement(2034..2351)
                     /gene="sugE"
                     /locus_tag="RH033_004577"
     CDS             complement(2034..2351)
                     /gene="sugE"
                     /locus_tag="RH033_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDV2993647.1"
                     /translation="MSWIVLLIAGLLEVVWAIGLKYTHGFTRLTPSIITIAAMIVSIA
                     MLSWAMRTLPVGTAYAVWTGIGAVGAAITGILLLGESASPARLLSLGLIVAGIIGLKL
                     STH"
     gene            3079..4119
                     /locus_tag="RH033_004578"
                     /pseudo
     CDS             3079..4119
                     /locus_tag="RH033_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606836.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4165 domain-containing protein"
     gene            complement(4171..>4479)
                     /locus_tag="RH033_004579"
     CDS             complement(4171..>4479)
                     /locus_tag="RH033_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HDV2993648.1"
                     /translation="RFINTDKAPAYGRALALLKREGRCPSDVEHRQIKYRNNVIECDH
                     GKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPLGEMRLVSRVFEM
                     "
BASE COUNT         1157 a         1123 c         1091 g         1108 t
ORIGIN      
        1 gtatcattgc agcaaagatg aaatcaatga tttatcaaaa atgattgaaa ggtggttgta
       61 aataatgtta caatgtgtga gaagcagtct aaattcttcg tgaaatagtg atttttgaag
      121 ctaataaaaa acacacgtgg aatttaggaa aaacttatat ctgctgctaa atttaaccgt
      181 ttgtcaacac ggtgcaaatc aaacacactg attgcgtctg acgggcccgg acaccttttt
      241 gcttttaatt acggaactga tttcatgatg aaaaaatcgt tatgctgcgc tctgctgctg
      301 acagcctctt tctccacatt tgctgccgca aaaacagaac aacagattgc cgatatcgtt
      361 aatcgcacca tcaccccgtt gatgcaggag caggctattc cgggtatggc cgttgccgtt
      421 atctaccagg gaaaacccta ttatttcacc tggggtaaag ccgatatcgc caataaccac
      481 ccagtcacgc agcaaacgct gtttgagcta ggatcggtta gtaagacgtt taacggcgtg
      541 ttgggcggcg atgctatcgc ccgcggcgaa attaagctca gcgatccggt cacgaaatac
      601 tggccagaac tgacaggcaa acagtggcag ggtatccgcc tgctgcactt agccacctat
      661 acggcaggcg gcctaccgct gcagatcccc gatgacgtta gggataaagc cgcattactg
      721 catttttatc aaaactggca gccgcaatgg actccgggcg ctaagcgact ttacgctaac
      781 tccagcattg gtctgtttgg cgcgctggcg gtgaaaccct caggaatgag ttacgaagag
      841 gcaatgacca gacgcgtcct gcaaccatta aaactggcgc atacctggat tacggttccg
      901 cagaacgaac aaaaagatta tgcctggggc tatcgcgaag ggaagcccgt acacgtttct
      961 ccgggacaac ttgacgccga agcctatggc gtgaaatcca gcgttattga tatggcccgc
     1021 tgggttcagg ccaacatgga tgccagccac gttcaggaga aaacgctcca gcagggcatt
     1081 gcgcttgcgc agtctcgcta ctggcgtatt ggcgatatgt accagggatt aggctgggag
     1141 atgctgaact ggccgctgaa agctgattcg atcatcaacg gcagcgacag caaagtggca
     1201 ttggcagcgc ttcccgccgt tgaggtaaac ccgcccgccc ccgcagtgaa agcctcatgg
     1261 gtgcataaaa cgggctccac tggtggattt ggcagctacg tagccttcgt tccagaaaaa
     1321 aaccttggca tcgtgatgct ggcaaacaaa agctatccta accctgtccg tgtcgaggcg
     1381 gcctggcgca ttcttgaaaa gctgcaataa ctgacgatga ggcccaggat attgggcctc
     1441 ctttctttct ctttttttcc tgttgtcatc tacacttaac aaaaatacag caaggaaaat
     1501 cccatgcgca ttttgcccgt cgttgctgca gttacggctg cattcctggt tgtcgcgtgt
     1561 agctccccga caccgccgaa aggcgttacc gtggtaaata actttgatgc caaacgctat
     1621 ctgggaacct ggtatgaaat tgcgcgcttc gaccatcgtt tcgagcgcgg attggataaa
     1681 gtgaccgcaa catacagctt gcgcgacgac ggcggcatca acgttattaa caagggctat
     1741 aaccctgaca gggagatgtg gcagaaaacg gaagggaaag cctatttcac cggcgaccca
     1801 agcagagccg cgcttaaggt ttcttttttc ggccccttct atggcgggta taacgtaatt
     1861 gcactcgacc gggaatatcg tcacgcgctg gtttgtggtc cggatcgcga ctacctgtgg
     1921 atcctttcac ggacccctac tatttcagat gaaatgaaac agcaaatgtt agccatcgcg
     1981 acccgggaag ggtttgaagt gaataaactg atttgggtga aacagcctgg cgcttagtga
     2041 gtgctcagct tcagaccaat aatgccagca acgatcagcc caaggctcag caaacgtgcc
     2101 gggctggcag actcacccag cagcaaaatc cctgtaatgg ccgccccaac agcgccaata
     2161 ccggtccaga ccgcataagc ggttcctaca ggcaacgtgc gcattgccca agagagcatg
     2221 gcgatactga cgatcatcgc cgcaatagtg ataatgcttg gcgtaagacg cgtaaaaccg
     2281 tgggtgtatt tcaggccaat cgcccagaca acttcgagca aacctgcaat taataaaacg
     2341 atccaggaca tatcaggctc cagaacaatg gggccgtccc cggtgaaaga agcgtttgca
     2401 ggtcgtcctg caaagctaat gtgtgaaatg gcatttttgc ccggaagaaa atgaatttca
     2461 acctttttat tcaccgcctg ctaaaagcaa gaattaagca taattagcgg cgtagttccc
     2521 gcatttggca ctcatcggag ttctctatga tgaagggctt tctgaaacca gaaagtcgat
     2581 tgcccctctt ctgaatgcga aacgtttatg cttaaaattt tagtgattga ccggtgccac
     2641 tttacccgca cagggataga ggccttgctt aatcattctg gcaggttcag ctcctcattt
     2701 ctggtatcag gaatcaataa tctcctgtta gcaaaagagc atattttgca atggaaaccg
     2761 catctggtga tcgcggattt atacagtttt attagcgaga cgcactccag tccgccaatt
     2821 aaaccttttt ttatgagttg tggcgtcatc ccactaattt tactgcaatc agcagacaga
     2881 cagcatgcac ccatcgtgcc ctctcaatcc gtagcccatt cggtcttgac caagcacacc
     2941 acgctgaaca cactctccca cacaattcag gatttcctta tttgctcaaa aagggcactt
     3001 ccacagccca tgtttatgca ctccctcccg taacctccag cattgggttc ttgtaactca
     3061 aaacgaatgg agaatgcgat gatcaatttc aagcctaaaa ttccggcgat gcttggggct
     3121 ctggcggttc ttaccgctgg tgctgctcat gcagagctgc tggaatacac ctttaaggct
     3181 ccagatggta cgcaacggtc tctgactccg aatgccaact atgccaaccc aacgggcaat
     3241 atctcgtttg ccttgagtgc cggtatcgac cgaaaggtaa agatttcggt gcttcggtcg
     3301 gatggaacag tggtttcgac agcgaccagc caccttcttg gggctactga tcgcatcaca
     3361 gtgggtggaa aatcctacta tggtgcggaa cttcaactac cagctccagt tggtggggca
     3421 tacactatca gggcggaaat tcttgcctct gatggttcta cagtacagac ggatgaatac
     3481 cctcttactg tcgatgtgac gccgccaacc tactcttcgc ttgccccggt ttatagtaac
     3541 tatgggcagg ttactagcgg tgatgtttgg aaactgggtc taggtggctc tgaggataat
     3601 gcctttttgc tgtcaggaat ctctgatgag tcacccatca aaggcgtcaa agcaaagctg
     3661 tatcgtcaag acggttcact ctacaaagat gtatccgtga actacgatga cgctaacgga
     3721 caagctagac agtctttcca atctggcttt ttcccagcct ctgacttgga tgaagtgttt
     3781 acacttcagt ttgagataag cgatagcgct ggaaatagct atctttcacc tcgtcaaaaa
     3841 gtgatgtttg atagcataac caatgctccg tctgcacctt ttggtgttta tgacccttca
     3901 agtacaaaca acttaggccc tggattaacc ggatttgttg cttacactga gggcatgaca
     3961 gttaaaacta acccaatcaa gttagcttgg cgtgttccgc gtgataactg gcatgagtat
     4021 agagaaggtg ggataaatat gaccaatgcc ttgggagaga tgtccaaggt aggagaagat
     4081 gccagctatg tttatctcgt cacaaccgca ccttatgggg cactgttgca aagttagcga
     4141 tgaggcagcc ttttgtctta ttcaaaggcc ttacatttca aaaactctgc ttaccaggcg
     4201 catttcgccc aggggatcac cataataaaa tgctgaggcc tggcctttgc gtagtgcacg
     4261 catcacctca atacctttga tggtggcgta agccgtcttc atggatttaa atcccagcgt
     4321 ggcgccgatt atccgtttca gtttgccatg atcgcattca atcacgttgt tccggtactt
     4381 aatctgtcgg tgttcaacgt cagacgggca ccggccttcg cgtttgagca gagcaagcgc
     4441 gcgaccatag gcgggcgctt tatccgtgtt gatgaatcg
//
LOCUS       DAPHCV010000217         4469 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.219, whole genome shotgun sequence.
ACCESSION   DAPHCV010000217 DAPHCV010000000
VERSION     DAPHCV010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.219"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..164)
                     /locus_tag="RH033_004580"
     CDS             complement(<1..164)
                     /locus_tag="RH033_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HDV2993649.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSR"
     gene            complement(232..447)
                     /locus_tag="RH033_004581"
     CDS             complement(232..447)
                     /locus_tag="RH033_004581"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993650.1"
                     /translation="MSGIIRALCHRAHQSSTTRDVALDRHLPDCEDILKEVIWAFSDF
                     VRDHRGVYDPEARYLAGNPWYPVTGQF"
     gene            complement(552..959)
                     /locus_tag="RH033_004582"
     CDS             complement(552..959)
                     /locus_tag="RH033_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HDV2993651.1"
                     /translation="MADFTGELHRLISEYLQCKRRKDQSVEPTPLYYLWSREAVANDT
                     TPYLMVLLLNNDVWCKSREVVVLRNLIEQAWGKVLGEDNSITHLVVYPEVLSLVFDAA
                     NTYNAEPGVLEQTARAYIESHGTIERHLGCSDF"
     gene            complement(1590..2015)
                     /locus_tag="RH033_004583"
     CDS             complement(1590..2015)
                     /locus_tag="RH033_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993652.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLYALSEQQKAACVVLNPEKSHPYIEDMEMFLSPLPVVLEWHCMMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFQDCGGAST"
     gene            complement(2012..2395)
                     /locus_tag="RH033_004584"
     CDS             complement(2012..2395)
                     /locus_tag="RH033_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993653.1"
                     /translation="MYAKSFIAFDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASRHCRQC
                     HHDYYGERYCTHCHTGHFSEEGVAE"
     gene            complement(2767..3366)
                     /locus_tag="RH033_004585"
     CDS             complement(2767..3366)
                     /locus_tag="RH033_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HDV2993654.1"
                     /translation="MKTYNTNPGYEMDPQLLTHFNQHLDSLFGVYSKLLPFRMDFAYR
                     KNTLSYRCACRYAMCAEILRLINEVGEKLVGYAWVMEYTERKGLHIHFVGYLNGQSHR
                     SSYLVSRLMGDIWRRVTGGDGYYHWCRFNKNYPVNINHVIHYSDHKAVNALRYALSYL
                     AKREQKECGIILKCSGLPEKSNRGRPRLGSTLPETYSLV"
     gene            complement(3598..3747)
                     /locus_tag="RH033_004586"
     CDS             complement(3598..3747)
                     /locus_tag="RH033_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
                     /protein_id="HDV2993655.1"
                     /translation="MEKVISHARYKLTPEEMEAFNSAVDHRLAELTMNKLYDRVPASV
                     WKYVT"
BASE COUNT         1223 a         1087 c         1047 g         1112 t
ORIGIN      
        1 cggctgctgc gccccagttt gatgggttta gggaaatggc cttcactgat caacttgtaa
       61 aaccatttgt cagtcatacc ggtgaaactg gtgatatagt tcatgtcgat cagggaatta
      121 tcggcacgca gaaatacgct gtcgtggctt gttgtagtca acacggtcag aatgtcccct
      181 gtgagattta tgaagattgt gcgaatgcgg ggaatctgct gagctgtgct ttcagaactg
      241 gcctgttacg ggataccagg gattaccggc gaggtaacgg gcttccggat catacacacc
      301 tcgatgatcg cggacaaaat cactgaacgc ccatatcacc tctttaagta tgtcttcgca
      361 gtccggtaaa tgacgatcca gcgccacatc ccgggtggta ctgctttgat gcgcccggtg
      421 acacaaagcc cggattattc cagacatcct gaatcaaacg ccccagatta ggggcgtcga
      481 aatatgcctc tctgaccatt atattccggc gtacaggtag caggtcagaa gtgacaatgc
      541 gtcacctgac gttaaaagtc actacaccca agatgacgtt caatggtacc atgtgattca
      601 atgtaagccc gggccgtctg ttccagtaca ccaggctcag cgttgtatgt gttagctgca
      661 tcaaatacca acgacagcac ttcaggatac acaaccagat gtgtaatgga gttatcttca
      721 cccaatactt ttccccacgc ctgctcaatc agatttctga gaaccaccac ctcacgactc
      781 ttacaccaga catcgttatt aagtagcagc accataagat aaggagtggt atcgttagcc
      841 acagcttccc tactccagag ataatataaa ggggtgggct caacagattg atctttacgt
      901 cgcttacact gcaaatattc agaaatgagt ctatgcagtt caccagtaaa atcagccatc
      961 agagagggaa tggccttatt aataccctgc cctggtatta atttaaattg taataattta
     1021 atttcaggat gtgtggctgc agcccgatac agagttgcaa ggacacactt ttgccagagg
     1081 gcgttacagg aaagcttaac atttgattct gtatacataa taaatcacct tacagttaca
     1141 acaggtcaaa aaccgctgta gccagagtta cgctggcctg atgctttagt accgggcatc
     1201 gtctgataat ccagacgctc caataagcgc tgatactgct cagggaaatc aggatcatga
     1261 atatcctgga tgtcacgtcc attagcaggg aaataaacaa tgcagccccc tggattaact
     1321 atgcagaaat cgtcctgagg cactgataaa tacggagagg actctcgcgt atggttggtt
     1381 gacaccacag tgcggattcg gcgaatccgc gatcacggtg cgatttcgca ccacagtaca
     1441 caatcaatac cccgggtttt attcaggtaa gcaggactgc ggatatccgg tgtcgcgcct
     1501 ttgtgtcacg aacggggtgg gtgcgaaaca ccagataaaa tgcaggttcc gatacctctg
     1561 aacgccctga gcaggtcggg catttcggat taagtactcg cacctccgca gtcctgaaac
     1621 aagtctggct ggtagctgta aacagacttc gtacataccg ctctgcaata aatccccgta
     1681 ccacaggcct cacagaattt cttcccatga aaaaactgct cacacatcat acaatgccac
     1741 tccagcacga ccggtaatgg cgataaaaac atctccatat cctcaatgta agggtgggac
     1801 ttttccggat tcagcaccac gcaggctgcc ttctgttgct cgctcagggc atataaatca
     1861 tgctcaaacc atgccccctg agcatctgtc tgcaaaatca accggccatg acaggaaagg
     1921 cagaaacact gccggatatt tctgctgagt gcagaatcag cactgattat gtgttcaccc
     1981 ggcgtgatcc ccagccccgt ttttataccg ttcattctgc caccccctcc tcactgaagt
     2041 gcccggtatg gcagtgcgtg cagtaccgct ccccataata atcgtgatga cattgtctgc
     2101 agtgccggct ggctttacgc accacgggta aggcatccgg tacgaattgc tgcagacgct
     2161 taatcagttg tatctccctg cgttccggtc tgacataagg gcactgctga ccgtgctccg
     2221 tcagcccgtc gtcagtgtgt tcaaaccagg gacgctcagt gtcgtattgc gggtggtatc
     2281 tgagcgcact gccgcaaagg tggcaagtgt agcgatcgta aggtgcagtc tgtgcggtac
     2341 gggcacccgt cagacgccca ttgccatcaa atgcaataaa ggattttgcg tacatcgtca
     2401 tattcctcag ccgccagacg acacgcaggc aacagctgtc ccggtacggg cagtgtggtc
     2461 agggggtgaa tggcggttca gttaaaatct ggactggagt ttgggtaaag caggctggtt
     2521 gctgttacag cactgtggtt gtcacacaat ggcccaccgg cgtaaaaaac tgtcagtagg
     2581 cagggatgga catcggggga tgcgctgtat cggttgccgg aaggaatggt aaagaatgtg
     2641 ccatggttaa atctccgtat ggttcatacg gaatagccat gtcgtaaaaa atgcgcagag
     2701 tccctgaagt ggcctccgga accgaccgta acaattagcg ccagcccgta gttgtcgttc
     2761 agggtcttac accagagaat aagtctccgg cagggtgctg cccagtcgcg gtcgaccccg
     2821 gttacttttt tcaggaagcc cgctgcattt caggatgatg ccacattctt tctgttctcg
     2881 tttggccagg tagctgaggg catagcgtaa ggcattcacc gctttgtgat ctgaatagtg
     2941 aatgacgtgg ttaatgttta ccggataatt tttattaaac cggcaccagt ggtagtaacc
     3001 atcccccccg gtaacccgcc tccagatatc tcccatcaat ctggatacca gatacgaact
     3061 cctgtggctc tgaccgttca gatagcccac aaaatgaatg tgaagccctt tacgttccgt
     3121 atattccatt acccaggcat acccgaccag tttctctccc acttcgttaa tcagtcgcag
     3181 tatctccgca cacatggcgt acctgcaggc acacctgtag ctgagggtgt tttttcggta
     3241 agcaaaatcc attctgaacg gtaacagttt cgaatatacc ccgaataatg aatccagatg
     3301 ctggttaaag tgtgtcagga gctggggatc catttcgtaa ccgggattgg tgttgtaagt
     3361 cttcatgttt cttcctcgtg ttctgtaaca caggaagaag cgctggcgta atttttgaag
     3421 aaaacgttat cacacgaaga cgtgcaggtt cacaactccc cgggcgccac agagaaagtg
     3481 ggaccacacc cgggtatcag aaccatacag agaacggcat acgtgacagt caatcacagc
     3541 gcagataata tctgaagtga tgtcattgtg acgactgtac ttcatcagaa acatttatca
     3601 ggtaacatat ttccagactg aagcgggaac acgatcgtaa agtttgttca tggtcagttc
     3661 tgccagccgg tgatcgaccg cagagttgaa ggcttccatc tcctctggag taagcttata
     3721 acgcgcgtgg gaaatcactt tttccagtga acaacagtgg aactactctg gtttaaaatt
     3781 ccattaccct ctcgttatct tgtactgtga ttaacgtaat atcgtcataa tggcctgacg
     3841 ttatcccgaa agcctttcca cttccctgtt tctccggtaa aagatgtttt taatactgaa
     3901 ggcataacgc cataaccaga tattacaatt aacgtagctt tccgcaacgt actacatgag
     3961 taatagaggt acagggttaa acaacactcc ggaagacttt ttaatgacat gaggcatact
     4021 cttttacgta ttactaccaa aactcatcag gtgagctgca ggaacagatc attaacagta
     4081 attccttaat caccgttatc cacagtatca acactggcga agtcatactc ttagctataa
     4141 aggtgatgac gggttcagaa tagttattac accatacaac aaaatacaga aaacaacagg
     4201 aaaaataaca gaaaaaaata gcagacatta cttaacaaag cacgttacag tcagtgtgtg
     4261 aacattacag atttatattc aggtgtggcg ggactacatt actgaagaga gagttaaggg
     4321 cagagttaca agtactacta cagagaatat cgctgcacag gaatattgtt cgatgagaca
     4381 gacttctgca ggacttggtt acaaggtgta tttacgaggt agaattacag gatagaatta
     4441 caaggtacaa tgacagagtt catttacac
//
LOCUS       DAPHCV010000218         4436 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.220, whole genome shotgun sequence.
ACCESSION   DAPHCV010000218 DAPHCV010000000
VERSION     DAPHCV010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.220"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1753)
                     /locus_tag="RH033_004587"
     CDS             complement(<1..1753)
                     /locus_tag="RH033_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HDV2993656.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMRVADAVAQYMRVPLGAGNSV
                     PWDPLVAPYVIEPMNCLVSREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKRLARTFRVSPEVVSRLSPNKNDNNVYDRTFLAGNYLKIGWPSVNI
                     MSSSDYKCVALTDYDRFPEDIDGEGDAFSLASKRTTTFMSSGMTLVESSPGRDVKDVK
                     WRRTSPHEAPPTTGILSLYNRGDRRRWYWPCPYCGEYFQPCGDVVAGFRDIADPVLAS
                     EAAYIQCPSCSGRIMPEQKRELNGRGVWLRDGESINADGSRYGDPRRSRIASFWMEGP
                     AAAYQTLSQLVYKLLTAEQEYETTGSEETLKTVINTDWGLPYLPRASMEQRKSELLEQ
                     RAEPVPSRSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRG
                     DSDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDN
                     AYKFWRRCRRDGLGKRIYLFKGDSIRRAKLITRTFPDNTGRTGRRAQAAGDVPLWLLQ
                     TDALKDRVNNALWRDSPG"
     gene            complement(1753..2247)
                     /locus_tag="RH033_004588"
     CDS             complement(1753..2247)
                     /locus_tag="RH033_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HDV2993657.1"
                     /translation="MDRELKNLTLNISQLAALSGVHRQTAAARLQNLPVAGGHESNLK
                     LYRVVDIVSAFLALPPPVAESEMDAHERKAWYQSERERLKFEQETAQLIPASDVRREF
                     AIWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLRDQIALRVTEAGADDEEEL
                     QQEE"
     gene            complement(2801..3007)
                     /locus_tag="RH033_004589"
     CDS             complement(2801..3007)
                     /locus_tag="RH033_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993658.1"
                     /translation="MNKEQSADELSLDLIRVKNMLNSTISMSYPDVVIACIEHQVSLE
                     AFRAIEAALVKHDKNSKDYSLVVD"
     gene            3208..3618
                     /locus_tag="RH033_004590"
     CDS             3208..3618
                     /locus_tag="RH033_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HDV2993659.1"
                     /translation="MAQAAIFKEIFDQVRKDLNCELFYSELKRHNVSLYIYYLATDNI
                     HIVLENDNIVLVKGLKKVVNVKFSRNKHLIETSYNKLKSKEITFQQYRENLAKAGVFR
                     WVTNIQEHQRYYYAFDNSLLFTESIQKTTQILPR"
     gene            complement(3770..3943)
                     /locus_tag="RH033_004591"
     CDS             complement(3770..3943)
                     /locus_tag="RH033_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HDV2993660.1"
                     /translation="MNIEDLKTKAEADISEYITKKIIELKKKTGKEVTSIQFTAREKM
                     TGLESYDVKINLI"
     gene            complement(4115..4270)
                     /locus_tag="RH033_004592"
     CDS             complement(4115..4270)
                     /locus_tag="RH033_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993661.1"
                     /translation="MREYPNGEKTHLTVMAAGFPSLTGDHKVIYVAADRHVTSEEILE
                     AAIRLLS"
     gene            complement(4350..4415)
                     /locus_tag="RH033_004593"
     CDS             complement(4350..4415)
                     /locus_tag="RH033_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993662.1"
                     /translation="MKAPSGAFLLGVYSMDTHILR"
BASE COUNT         1217 a         1130 c          926 g         1163 t
ORIGIN      
        1 gacctggcga gtcacgccat aacgcgttat tcacccggtc tttcagtgca tccgtctgaa
       61 gaagccagag cggcacatca cctgcggcct gcgcccgtcg gcccgttcgt ccggtgttat
      121 cagggaatgt acgggtgatc agttttgcgc gccggatgct gtcgccctta aacaggtaaa
      181 tacgtttacc aaggccatca cgacggcaac gacgccagaa tttataggca ttatcagtga
      241 ccccgtcttc accgccggag tccaccgcca ttgccatcag tcgcatttgt tgagaaggat
      301 cggaggccag cggccagctt ttatgaaaaa catccgtcag caggacatcc cagtcttccg
      361 gatagctggc cggatcaatt cgctggctct ccccgtcgct gtcaccgcgc aatgactgcg
      421 tgatgttgta acgatcaata atccagcgtt cgccacggct gccatagccc gttacctgaa
      481 ccacaaaacg gcgatgacgt cccgcctgca catccactgt cgccacaagg aaattaacgc
      541 catccggcac actgcgggaa ggaactggct ctgcccgctg ctcaagcagt tcactttttc
      601 gttgctccat gctggcgcgg ggaagataag gtaatcccca gtcggtattg ataaccgtct
      661 tgagtgtttc ttcacttccg gttgtctcgt attcctgttc tgcagtaagc agtttgtaaa
      721 cgagttgcga gagtgtctgg taagcagctg ccggcccctc catccagaat gacgcaatac
      781 gtgagcgtcg gggatcacca taacgactgc catccgcatt gatggattca ccatcccgca
      841 accagacccc acgtccgttc agctcacgtt tttgttcagg cataatccgt cctgaacagg
      901 aaggacactg aatataagcc gcctcacttg ccagcacggg atcggcaata tcacggaaac
      961 cagcaaccac atcgccgcag ggctgaaaat actcaccaca gtatggacag ggccagtacc
     1021 agcgacggcg atcaccacgg ttatagagcg acagtatccc cgtggttggt ggagcctcat
     1081 gcggtgaagt ccgtcgccat ttcacatcct tcacatccct gccgggggaa ctctccacca
     1141 gcgtcatacc actggacata aatgttgtgg tacgttttga ggcaagagag aaagcatccc
     1201 cctcgccatc aatatcttcc ggaaaacggt cataatccgt cagcgcgacg catttataat
     1261 ctgatgagga catgatattg actgacggcc agccgatttt caggtagtta ccagcaagga
     1321 atgttctgtc ataaacgttg ttgtcatttt tgttcggact caggcgactg accacttccg
     1381 ggctgacgcg aaacgttctg gcgagtcgtt ttttggagtg ttcgcgggct ttttcctccg
     1441 tcatctgaat gatcagcata tcagcaggat cgcaaatcac gttgtaaatc acccagccgt
     1501 caatcaggcc gatagtcttg ccggttcgtg ccgggccaac aaatatcact gcgtcgtatt
     1561 cacgcgagac caggcagttc atcggctcaa taacatacgg tgccaccagc ggatcccacg
     1621 ggactgagtt ccctgccccc aggggcaccc gcatatactg agcaacggca tcagcaaccc
     1681 gcattcgtct cggtgcgcga aggatataac ctgaatcggt tcgtgctgcc tttgcggttt
     1741 cctgattcag cattactcct cctgctgtaa ttcctcctca tcatccgcac ctgcttcagt
     1801 cacccgcagg gctatctgat cgcgcagatc atcaataatg gactgaacac ggctcacagc
     1861 ggcaggctgc agaccgcagt cacgttcaag aatatccggt aatgtctcca gcacctgcac
     1921 gaccgctttt gcccagatgg caaactcccg tctgacatca ctggccggaa tgagttgtgc
     1981 cgtttcctgt tcgaacttaa gacgctcacg ttcagactga taccaggctt tgcgctcatg
     2041 cgcatccatt tcgctttctg caaccggcgg tggtaatgcc agaaatgccg acacaatatc
     2101 aaccacccga taaagcttga ggttgctttc atgcccccct gcaacgggta gattttgcag
     2161 ccttgccgca gcagtctggc gatgtacacc tgacagtgcc gccagttgac tgatattcag
     2221 cgtcagattt tttaactctc gatccatacc cgctccagaa tgttttaaac atgcatcttg
     2281 cgaacaactt taggcaaacg gtgttagtga tgaacaaaaa acaatcaaaa tcgacaccac
     2341 aaaaataaaa acactgtaat atcaatctat tacagtagtg gtgatgacga atgaaatttc
     2401 aaaaactagc ctttttccgc gacgctcccg ccccgtggca ggggccccca ccgggaggac
     2461 ccgacagcct gacagccgtg acgagcatct gatacagcgc tttacataat ggcataggaa
     2521 taattcagaa ggacatcaca gcatgcccac acaaattagt gtgagtgtcc tgtttcttcc
     2581 tacgcacagg actgacgagc atgaggggaa aatatgcgaa ccataaatgc ctcatcttca
     2641 gcaatgcagc cggcatccga acaggacgat atgtaaattc acctaattac gaggacattg
     2701 cagtagttga attgcagctc tgtattagcc tgacagtgac agaatgcgac attgactctg
     2761 tcacaggtta aatagtttga atgattagca gttatggtgc tcagtcaacc accagggaat
     2821 aatccttcga attcttatcg tgcttcacca acgctgcctc aattgccctg aatgcttcca
     2881 gagacacctg atgttctata catgcgatta caacatccgg gtaactcata gaaatggtgc
     2941 tattaagcat attttttaca cgaatcagat ccaacgagag ttcatcagca gattgttctt
     3001 tattcatttt gtcgctccat gcgtttgctc gtcatctagc ggttaaaata ttacttcaaa
     3061 tctttctgca taaaagagga aaagtatgaa agtttgagta catcgacctt acatacatct
     3121 gtcggttgca tatccctcct ggatgccagc aaggctcaac tttgttacgc aaactacgcg
     3181 ccatcgtatc acatggaagg tttaccaatg gctcaggctg ccatttttaa agaaatattc
     3241 gatcaagtgc gtaaagattt aaactgtgaa ttattttatt ctgagctaaa acgtcacaat
     3301 gtctcacttt atatttacta tttagccaca gataatattc acattgtgtt agaaaacgac
     3361 aacatagtgt tagtaaaagg actcaaaaag gttgtaaatg ttaaattctc cagaaacaaa
     3421 catcttatag agacctccta taataagttg aaatcaaaag aaatcacatt tcaacaatac
     3481 agggaaaatc ttgctaaagc aggagttttc cgatgggtta caaatatcca ggaacaccaa
     3541 agatattact atgcctttga taactcatta ctatttactg aaagcatcca gaaaactaca
     3601 cagatcttac cacgctaaac cataacgtcc ggcttctctc actcctgagc cggactgcat
     3661 tggtttaata aaaaccatca acaattgtga tttagatatt cggaaccatt caaatataac
     3721 aaaaccccgt aaaaacgagg tttatggata aactttatta ttgaatacat cagattaaat
     3781 taatcttgac atcatagctt tcaagacccg tcattttttc ccgtgcggta aactgaatac
     3841 tggtaacttc tttcccggtc tttttcttaa gttcaataat tttttttgtt atatattcag
     3901 aaatatctgc ttctgctttt gtttttaagt cttcaatatt catcatttcc tcttttagtc
     3961 tgttatgact ttccagttac acagtaagtc gattatatgg tgcaaacgtg taaaagataa
     4021 gatgaaacat cgcaataatc aacatacgat agtctaaatt ttacacaaac agacaaagag
     4081 aattttcctg aattatcaat gcaatagcat caaatcaact caagagcctt attgctgctt
     4141 ccagaatttc ttctgaagta acatgtcgat ccgcggctac ataaatgact ttatgatctc
     4201 cggtcagaga tggaaaccct gcggccatta cagtaaggtg tgttttttcg ccatttggat
     4261 attcacgcat gatggtgtta actccagtca tcgctgacac taccactgct ggttcagagt
     4321 taaaaaaaac tatgattttt ttcatgatgt taccgtagta tgtgagtatc catcgaatag
     4381 acaccaagca aaaaagctcc cgaaggagcc ttcattttca cttttttaaa tccaac
//
LOCUS       DAPHCV010000219         4434 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.221, whole genome shotgun sequence.
ACCESSION   DAPHCV010000219 DAPHCV010000000
VERSION     DAPHCV010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.221"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            50..355
                     /locus_tag="RH033_004594"
     CDS             50..355
                     /locus_tag="RH033_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993663.1"
                     /translation="MTETGGQPPVSFPVKDVAGLLFLLRRLTRRGRNAACGQCPASGR
                     ARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGIALPSKRRRLRYSTVGLTRYRTR"
     gene            complement(584..2284)
                     /gene="ltrA"
                     /locus_tag="RH033_004595"
     CDS             complement(584..2284)
                     /gene="ltrA"
                     /locus_tag="RH033_004595"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDV2993664.1"
                     /translation="MTAKNINAGAPTDIVTHWHNINWAKCRREVRRLQARIVKAIQEE
                     KAGKVKALQWLLTHSFSARAMAVKRVTDNRGKRTPGVDGKTWSKPGSKMKAIYTLKRR
                     GYKPLPLRRIYIPKSNGKKRPLGIPTMKDRAMQALYLMALEPVAETTADPNSFGFRPC
                     RSTADAIEQCFTTLHRADRAQWILEADIRSCFDEISHEWLIANIPTDTAILKRWLKAG
                     YIDLGKLYPTSAGTPQGGIISPTLANMVLDGLQPLLKKTFYRGGLNPEKINIIRYADD
                     FVITGISHDTLSEKVLPLLENFLAERGLTLSPEKTRITHISDGFDFLGMNIRKYNGKL
                     LIKPSPQKVKEFLKRVRQTIKDNPTVSQSTLIWHLNPVLKGWARYYRHVVSKKIFQKV
                     SHEIWKALWRWACRRHPKKGTGWIRKKYFRSVNFRHWVFGTETGKLLPSGKRELLALY
                     EISDMPVKRHRQIKSTANPFDPSYESYFEARLKATMLESIQGYSRLRNLWLRQGGQCP
                     VCHLPVNATTGWQLRHIVPKSEGGLDNIGNLAMLHPACHHTAKHQGISVMKPALMWSY
                     "
     gene            2864..3517
                     /locus_tag="RH033_004596"
     CDS             2864..3517
                     /locus_tag="RH033_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HDV2993665.1"
                     /translation="MKLKQQTVRLSRRTLTDPPYLVGFRDRQGRTIAGDKTDEWLQPA
                     CNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYTSKAAYVGYRH
                     ECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESFTHPNAIVLDP
                     FAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLTAVQRAMQQGAANDNWFEPEAA"
     gene            3518..3739
                     /locus_tag="RH033_004597"
     CDS             3518..3739
                     /locus_tag="RH033_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993666.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            3753..4187
                     /locus_tag="RH033_004598"
     CDS             3753..4187
                     /locus_tag="RH033_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HDV2993667.1"
                     /translation="MYGTCETLCRALAAKYPGDTPLMLVIWSPEEIQALADGMDISLS
                     DHEIRTVLARLEDIPEDQRTESGISSGAAMEIINNVRENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDNGLAVPECVTRRQAVVNQARALLKNNTREND"
     gene            4232..>4434
                     /locus_tag="RH033_004599"
     CDS             4232..>4434
                     /locus_tag="RH033_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993668.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIYVL
                     LDAPQLATLLINRHGGVLPPLLAS"
BASE COUNT          918 a         1216 c         1176 g         1124 t
ORIGIN      
        1 cgcgccggag tggatagtcg acaaggtggc gcattacggt ggatgaagta tgacggagac
       61 tggcgggcaa ccgcccgttt cttttccggt aaaggatgtc gccgggctcc tctttttact
      121 gcgccggctg acgcggcgcg gcaggaacgc tgcctgtggt cagtgtcccg cgtccggccg
      181 ggcacgggac gggcgctttt accgctcccg gctggcatcg gtgacagtgt acgccagccc
      241 gtcgcccttt tctgatgaac gcccttcaag ccgctttcgc ggcatagcct tgccgtcaaa
      301 aagacgacgg ctgcggtatt ccacggtcgg cctgacccgt taccggacgc ggtgaacagc
      361 ccacaggcag cggggaacgg gcaccacagg ggtgccctcc cggtgccctc tgtaagagaa
      421 ggagtttggt atgtcccgtt ttgtcctcgg taactgcatc gatgttatgg cccgtatccc
      481 tgataacgcc attgatttca tgtcgggtag cagctgcaca ttactgtgca gccgccccct
      541 aagaaccgta cgtgccagtt tcccggcata cggctcaagc ctttcaataa ctccacatta
      601 gagccggctt cataacagat attccctgat gttttgcagt gtgatgacat gccggatgta
      661 acatggccag attacctatg ttatccagtc ccccttcgga tttcggcaca atgtgtcgaa
      721 gttgccagcc tgttgtggcg ttcactggca ggtggcagac ggggcactga cctccctgtc
      781 ggagccacag atttcttagg cgagagtaac cctgtatgga ttccagcatc gtagctttta
      841 gccgtgcttc aaagtacgat tcgtagctgg gatcaaaagg gttagcagtg ctttttattt
      901 gtctgtgtct tttgactggc atatccgaga tttcatatag cgccagtaac tcacgtttgc
      961 ctgatggcag gagcttccct gtttctgtgc cgaataccca gtgtcggaag ttaactgaac
     1021 gaaagtattt ctttcttatc cagcctgtac ctttctttgg gtgtcgtcga catgcccagc
     1081 gccatagtgc tttccatatt tcatgactga ctttctgaaa gatctttttt gaaacaacat
     1141 gacgatagta tctggcccag cctttgagga ctgggttgag atgccatatc agtgtgctct
     1201 ggctgactgt cggattgtct tttatcgtct ggcggaccct tttcaggaat tcctttacct
     1261 tttggggtga aggtttaatc aacagtttcc cgttatattt gcggatgttc atacccagaa
     1321 agtcgaatcc atcactgatg tgtgtgattc gtgtcttttc tggtgagagg gtaagccctc
     1381 tttctgccag aaagttttct aacaagggaa gaaccttttc tgacagcgtg tcgtggctta
     1441 tccccgtgat cacaaaatcg tccgcataac ggattatgtt gatcttttct ggattcagtc
     1501 cgcctcgata gaaggttttc ttcaacagag gctgcagacc atccagaacc atgtttgcca
     1561 gcgttggaga tattattccg ccctgtggag ttcctgcact ggttgggtat aatttcccca
     1621 aatcaatata tccggctttc agccatcgtt tcaggatcgc cgtatccgtt gggatattgg
     1681 cgattagcca ttcatgtgaa atctcatcaa aacaactgcg aatgtcggct tccagaatcc
     1741 attgtgcccg gtcagccctg tgcaacgttg tgaaacattg ctcaatggca tcggctgttg
     1801 agcgacaggg cctgaaccca aacgaatttg ggtcagccgt tgtttctgca acaggttcca
     1861 gcgccatcag atagagtgcc tgcatggctc tgtctttcat tgtcgggatc cctaatggtc
     1921 gctttttgcc gttagatttt gggatatata tacgccgtag cggaagaggt ttgtaacccc
     1981 ttcttttcag cgtgtatata gccttcatct tgctgccggg ttttgaccat gttttgccat
     2041 caacacccgg cgttctcttc cctctgttat ctgtcactcg tttaactgcc attgctctgg
     2101 cgctgaacga gtgggtgagt agccattgca aggcttttac cttgcccgct ttctcctcct
     2161 gtattgcctt cacgatacgc gcctgcagcc ttctgacctc gcgacggcac ttggcccagt
     2221 tgatattgtg ccagtgagtc acgatgtcgg tgggtgcacc cgcgtttatg tttttcgcag
     2281 tcatctgctt accctccttc aaaggtattc aggttatctc gtgttgaaag accatgcgga
     2341 agtctgcccg ctttcgcgtg gaatgatgtt gctgaaacag ctcaactcct gtccatccca
     2401 ttacaggatg gcattcgctt tctccgcact cctgtacccg catcactgac agctttcctc
     2461 gcggttagcc tgccctgagg gcagtgatac gggcttaccg tgttccgcac agtttgcagg
     2521 gatgggttag gttctgccta tccaccggta gctttgatgt ccgtgcctcc ccagtcaaaa
     2581 gaggaaggtc cagctacgtt cccattttgg gtcgggcctg tcagtgcctt tggcccgttc
     2641 agggttgacg atgtttatca gcagttcaca tgtgttaacc ctgccatcca gccttgcgcc
     2701 ccatccgccc tgacactggc agacatgctt cctcctcgcg gattccacat ggtgtgaaaa
     2761 ccgggctacg ttgtcccgaa ggcttcatac cgaacagtta cccgctctgc atgcttcggt
     2821 aggctacagg cgacggaacg cctggtttta ctacttcctt tacatgaagt taaaacaaca
     2881 aactgtgcga ctttcacgtc gcaccctcac cgacccgccg tacctcgtcg gttttcgtga
     2941 ccgtcagggg cgtaccattg ccggcgataa aaccgatgaa tggctgcaac cggcctgtaa
     3001 tgagatgtac cgcgtactca aaaaagacgc gttaatggtg agcttctatg gctggaatcg
     3061 cgtcgatcgt tttatgtctg cctggaaaaa tgcgggattc agcgttgttg gtcacctggt
     3121 cttcaccaaa aactacacat cgaaggccgc atatgtgggc tatcgccacg aatgtgccta
     3181 catcctggca aaaggccgtc cacgtctgcc acaaaacccg ctgccggacg tgctgggctg
     3241 gaaatattcg ggcaatcgcc atcacccgac ggaaaagccc gttaccagcc tgcaaccact
     3301 gattgagagc ttcacacatc cgaacgcgat tgtgctggac ccgtttgcag gcagcggatc
     3361 aacctgcgtg gccgctcttc agtccggacg ccggtatatc ggtatcgagc tgcttgaaca
     3421 gtatcaccgt gccggacagc aacgactgac cgccgtgcaa cgggccatgc agcagggggc
     3481 cgcgaatgat aactggtttg aaccggaggc tgcgtaaatg aactatgccg gacacgaaaa
     3541 acttcgcgcc gacgtggcgg aggtggccaa caccatgtgt gaccttcggg ccaggctgaa
     3601 cgatatggag catcgctgtc gctttgattc cgatgtgctt gttgaacgtc tgacacgcca
     3661 gacgctgtat cgcgccaatc gcctgttcat ggaagcgtat accgaaattc tggaactgga
     3721 ttcctgcttc aaagattgag aaggagacga gaatgtacgg aacatgcgaa acgctctgcc
     3781 gggcgctggc agcaaagtat ccgggagata cgccgctgat gctggttatc tggtccccgg
     3841 aagaaattca ggccctcgct gacggaatgg atatttccct gtccgatcat gaaatcagga
     3901 ctgtcctggc gcgcctggag gacatcccgg aagaccagcg gactgaatcc ggtatttctt
     3961 ccggtgcggc gatggagatc atcaataacg tgagagaaaa ccgccaggtg accgtccctg
     4021 ctgaactgct ggcgtccctg attcagaccg ccgaacaggc actgtggaaa cgtgagtggg
     4081 ccgcccgtga taacggcctc gccgtcccgg aatgcgtcac ccgccgacag gcggtggtta
     4141 atcaggcccg cgccctgctg aaaaacaaca cacgcgaaaa cgactgatgt tatcgccgcc
     4201 tgcgggcggc gattcaggga gaccatgaat catgaacgaa acgctgaatg cactgatttg
     4261 tcgccacgcc cgtaacctgc tgctggcgca gggctggccg gaagagacgg atgttgacca
     4321 gcgtaacccg aagtatccgg gctggatcag catttatgtt ctgctggatg cgccccagct
     4381 ggcgacgtta cttatcaacc gtcacggcgg cgtactgccg ccgctcctgg cctc
//
LOCUS       DAPHCV010000220         4272 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.223, whole genome shotgun sequence.
ACCESSION   DAPHCV010000220 DAPHCV010000000
VERSION     DAPHCV010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.223"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            159..1007
                     /locus_tag="RH033_004600"
     CDS             159..1007
                     /locus_tag="RH033_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDV2993669.1"
                     /translation="MSDITVSRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTALDWAMKQNFHCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNSPCKFGSKIIVNNLVRWDRWGLHLITGQQADRLADQERMLHLFSGKPIPDSR
                     ENIIIRLDDHIRSVQGKVCYEDEMFNIRYFKKGSAHITFRKPELVDRLNDIIAKHYAG
                     KVSSSV"
     gene            complement(1066..1269)
                     /locus_tag="RH033_004601"
     CDS             complement(1066..1269)
                     /locus_tag="RH033_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993670.1"
                     /translation="MLSKQIPLGIYEKALPAGDELNQAILLNNKYIFKHLPINSPQYS
                     PQGVVVIHGGILNSVEIFSVMVQ"
     gene            complement(1279..1929)
                     /gene="ulaD"
                     /locus_tag="RH033_004602"
     CDS             complement(1279..1929)
                     /gene="ulaD"
                     /locus_tag="RH033_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDV2993671.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIGQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(1943..2407)
                     /gene="ulaC"
                     /locus_tag="RH033_004603"
     CDS             complement(1943..2407)
                     /gene="ulaC"
                     /locus_tag="RH033_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HDV2993672.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(2417..2722)
                     /gene="ulaB"
                     /locus_tag="RH033_004604"
     CDS             complement(2417..2722)
                     /gene="ulaB"
                     /locus_tag="RH033_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HDV2993673.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            complement(2738..4135)
                     /gene="ulaA"
                     /locus_tag="RH033_004605"
     CDS             complement(2738..4135)
                     /gene="ulaA"
                     /locus_tag="RH033_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDV2993674.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIERMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
BASE COUNT         1122 a         1129 c         1038 g          983 t
ORIGIN      
        1 tgccctgcat tgaacaggcc agggaagata tccgtacact gcgccagttg cagcttctgc
       61 accagaaccg gtgactctca ctcaccagac ttcattccac tgacgccagc ctgaacaggg
      121 ctggcgtttt catttatctg caaaaaggaa tatcgattat gtctgatatc acagtctccc
      181 gtccggaagt tgtcaacggg catacggacg ttatctgctc aaccagtatc cgccacattc
      241 tggctgtacg aaagagtaca ctgctgcaaa tcgacacact tatccggcaa ctggctgaaa
      301 tctcagcaat gacagaaagc attggcggta aaaccgcact ggactgggcc atgaaacaga
      361 attttcactg cggttgctgg ctgatggaga aacctgaaac cgcaatgaaa gccatcacac
      421 gcaatctcga tcgcgaaatc tggcgtgacc tgatgcaacg ttccgggatg ctttccttaa
      481 tggatgcaca ggcccgtgat acatggtacc ggtcactgga atacgataat tttccggaaa
      541 tcagtgaagc gaacattctg agcacatttg aacaactgca ccagaataag gatgaggtgt
      601 ttgagcgagg agtgatcaac gtcttcagag ggctgagctg gaattacaaa accaattccc
      661 cctgtaaatt tggcagtaaa attatcgtca acaacctggt gaggtgggac agatggggat
      721 tgcatcttat caccgggcaa caggcagatc gactcgccga ccaggaaaga atgctacacc
      781 tgttcagcgg caaaccgatc cccgacagcc gggaaaatat catcattcgg ctggatgatc
      841 acatccgctc cgttcagggc aaagtgtgct atgaagatga gatgttcaac atcagatact
      901 ttaagaaggg ctccgcacac atcacgttca ggaaaccaga actggttgat cggctaaatg
      961 atattattgc gaaacattat gcgggaaaag tatcttcttc agtatagact tatatatcac
     1021 aggtattgtg tctcgtccgg aacaagacag gtaggtatgc tgcattcact gaaccattac
     1081 agagaatatt tcaacagagt tcaatatacc tccatgtata acaactacac cttgggggct
     1141 atattggggg ctatttatag ggaggtgttt aaatatatac ttattattca ataatatagc
     1201 ttgattaagt tcatccccgg cggggagcgc tttttcatag atgccaagcg ggatttgttt
     1261 ggacaacata tccgctcctt agccccacag ttcagcgatg gaacgtttga actgacgtgc
     1321 ggcctccacc ggagaagcgg catcacgaat gctgcgacca gcgataaaca catgaatcgg
     1381 aatgccttta aacaacggca gatcttccag cgccagacca ccggtgacgg tgactttgaa
     1441 gcccatatcg gaaagacgtt tgatcgcggt aatgtccgct tcgccccacg ccacacctgc
     1501 ggcctgtgcg tcacggctgc ggtgataaac cacctgccca atgcctgcgt cgcgccactg
     1561 ttgcgcctgt tcccaggtcc agtaaccggt cagttcgatc tgcacgtcgc cgttaaactc
     1621 ttttgccacg tccagcgcac ctttagctgt gttgatatcc gcacagcaaa tcacggtcac
     1681 ccagtcagcg ttagcttcga agcacatacg agaaaggatt ttgcccgcat cggcaatttt
     1741 ggcgtctgcc agtacgattt tgtgcgggta aagcgctttc agatcgcgaa ccgcacgcac
     1801 gccttcgccc acgcacagaa tggtacccac ttcgataatg tcgacttctt cggcaatcag
     1861 gcgagtggtt tcataggcgc tatccatagt ctggttgtcc agcgcgacct gcaacatcgg
     1921 taatgacatt ttcaattcct tcttaagctg ccgcgttggt gcggtcaatg agatccagta
     1981 cttcctgctc ggtacggcag gcacgtaaac ggtcgaaatt ctcttcatct tcaaacagat
     2041 tgacgatctg catgatgccc acttcctggt gagtgttggc atcgaccgcc gccatggtga
     2101 tgaggatatc caccggatcg ttatcttcgt ggttgaactc cagcggcttt ttcagcgtca
     2161 ccagcgcaaa accggttttc ttaacgccct cttccgggcg accgtgcggc attgccaggc
     2221 cgggtgcgag cacaaagtac ggaccgtgct gttcaaccgc atccagaatc gcctggtagt
     2281 aacgcggctc gacaacatcc gccgccacca gcaggtcaac gccgattttt actgcgtcct
     2341 gccatgtctc agcttcagcc tgcaggcgga tggatttatt ctccgccagc gaatcacgta
     2401 atttcatggc gcgtccttac ttcacgtcct gcggaaaatg ctctttgatc acttccagca
     2461 atttcgggcc aaagtcggca ggagagagca tattgcgcac gccaaccaca tatttgttgc
     2521 cggtcacagt gatttcgccc gcaatatggg tagaagcgat gatgatatcc gcgccgctca
     2581 actcgctttt gtattcgcca accgcgcagc tgtttaccgt gtggtcaatg ttcgactggg
     2641 ttaaaaactg gtccactttc attttcatga tcatggaact gccttgtccg ttgccacaca
     2701 cagccagaat acgtacggtc ataatcaaaa ctccttatta agcagactgt tctgccagtt
     2761 gtttttctgc atcttcttct gcgcgcagtg cgcggccagc gaagaacata taagccagtg
     2821 caattacaat gatgacagcc ataaaggcga taccgatgga gaagaagccc tgcatcatcg
     2881 gcggtgccag aatcgaccag tccgccatgc ccatccaggc gctcataccg gtgagtttca
     2941 ccgcccagac gcaaccaaag atttcgatca tccccatcac caggcaaatc ttcagcgccg
     3001 cgcgccagcc gccgaagtgg ttagcgaaca cgccgatagt ggcgttggag aagaacatcg
     3061 ggataaagcc tggaataatc aggattgaag agccacaggc gaccaggatg ccaaccgcaa
     3121 tcagttgccc gatggtgccc cacataaaac cccagaccac ggcgttcggc gcgaagctat
     3181 aaatagccgc acagtcaatc gccagaaccg cacccgggat caggcgctgg gagataccgt
     3241 tgaatgcttc agagagttcc gccacaaaca tgcgcacgcc ctgagtgatg atgaaaatcg
     3301 ctaccgcgaa ggagaaaccg gtttgcagga tgtagaccgt ccagttcact ttacccgcca
     3361 tcgcttgtac ggtgtcgata ccaaaggaga gcagaatggc accaaagaag atagtcatga
     3421 caatcgccgt ggagacgatg ttgtcgtgga aaatattcag ccagccaggc agtttgaggt
     3481 cttcaacgct ttcctctttt ttgccgagga acggtgcgac tttataagca atccatgatg
     3541 cgaactgctg ctggtgacca atggagaaac cacagccgtc ggtgacttcc tgtgtcggct
     3601 tgtacatcat gttggaggta atgccccagt agagcgaaac cagaatcgcg gtgcagataa
     3661 tggtcgtcca catggagtag ccgaagataa acagcgtaac ggcaatcagc cccgcctgct
     3721 ggaacatgat gtgaccagtc agcatgatgg tacgaatacc ggtaatgcga cgcagcagca
     3781 cgtaacagat gttcagcgcc agcgctaaca acaccgcgta acccacccag ctataggcat
     3841 cgcccatgcg ttcaatggtt gccatcatcg acgcgtaagt atcggaaatt gcgccgttaa
     3901 tgccgtagac ttcggacatt ttcgccacca ccggtttgaa ggtgctggtc aggatgccgg
     3961 accctgcctg caacaacatg aaaccaatta tggttttaat cgtaccttta ataataacgc
     4021 tgacactttt gcgcagtagg atgtaaccca gacaggtcac aatacccagc aacaacgggg
     4081 cattggtcat gacctggtta aaaaacacgg taaagatgtt gtagaggatc tccataacga
     4141 tctcctgaag aagaggtaac cgggtatttc gtacacccgg aatgttgtgc tctcactcta
     4201 attttcaaaa gtaatcacaa caagattatt tatgattaaa tgtgacgtcg cacgcaaata
     4261 attccatata aa
//
LOCUS       DAPHCV010000221         4246 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.224, whole genome shotgun sequence.
ACCESSION   DAPHCV010000221 DAPHCV010000000
VERSION     DAPHCV010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.224"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(164..677)
                     /locus_tag="RH033_004606"
                     /pseudo
     CDS             complement(164..677)
                     /locus_tag="RH033_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
     gene            complement(738..842)
                     /locus_tag="RH033_004607"
                     /pseudo
     CDS             complement(738..842)
                     /locus_tag="RH033_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(844..1167)
                     /locus_tag="RH033_004608"
                     /pseudo
     CDS             complement(844..1167)
                     /locus_tag="RH033_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1913..2890
                     /locus_tag="RH033_004609"
     CDS             1913..2890
                     /locus_tag="RH033_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDV2993675.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKLFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(3169..3909)
                     /locus_tag="RH033_004610"
     CDS             complement(3169..3909)
                     /locus_tag="RH033_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDV2993676.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGVRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLK"
     gene            complement(4030..4218)
                     /locus_tag="RH033_004611"
     CDS             complement(4030..4218)
                     /locus_tag="RH033_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HDV2993677.1"
                     /translation="MASDTFSCRRRLSAGFFSVSACSWHYPNSPKNGKPVLSPHHDWL
                     KTGSLRQNAPLRIWFYPQ"
BASE COUNT         1023 a         1086 c         1046 g         1091 t
ORIGIN      
        1 gctttttcgg ttgaaaagaa attgattgaa ttactggaaa aacaatctgg tctccatacg
       61 tctgtggatt gcagtactcc ccttacagag cagagtctgt cgagaaatga cggtgaagac
      121 cagaccagaa gaaacgctga aaatatgttc ggagatgact gaactaaccc agcctggcca
      181 ggggaatatc tcgccattta gttgtataac actgactcaa catttcccgt ttcatttgcc
      241 attcaggggc ggttcctcgc cccgcaaacc acactttccc ttttcccgac tggttcagtt
      301 catcgagtgt tttcattaac ttttcactgt ttttacgggg ctggatttca tcaaataatc
      361 ccggctgcgc tatacccgat ggtgtgaaat cagccagcat gactcctgcc ttcatatagc
      421 ggtacccttc acgccagaca tggtttaagg ctctgcatgc ggcggcaata atgtcccggc
      481 tgtcctgtgt gggcaatgaa agcttttcca cagcggcatt gctgtaacag ggttcagggg
      541 gatgtccgta caaatgtcgt cacctgccgg caatactgac gctccccacg tagtttctct
      601 gcggcccgct ctgcatactg aacaacagcc tggtgcatgg catctttgtc tgtgattcgt
      661 tcaccaaaac tgcgactatg tcaacgccac gatgtttgac cgttatttgc cattttcatg
      721 cccgctaaat actttcgtta ctgatgctgt ccgctttgca ccatttcatc atcactgatt
      781 ggcgttgtgt ttttggtcag cactccagct ttacgtttct cttttaaccg gtaactctct
      841 ccttgttctt tttgacggac gcattttacg tttgggctgg atgtagtaac gcagcatgga
      901 acgtccgccc gtataaccca ttgccttaat ctccgcgaag ataacctcgc tattccagac
      961 attctctgcc aggcgcatgt cgatgtaatc cataaacggt ttcagcttaa ccattttgtg
     1021 gcgggtcttt ctggctggcg gttcagggta tttgaggtag cgtctgaccg tccgttcaga
     1081 gcaaccaatc tgagccgcaa tatcgacaat gtacgcgccc tgctggcgca tttgctttat
     1141 catgtaaaag tcctctctgc tcagcatgtt gatgtccttt ctggtgtgag aaccttaagg
     1201 aaacaacatg ttgggtggag cggacaatcc aaatggtgaa ttaccgtctt atatcactgg
     1261 cgctgacacc tgtagtttgt atacacaatc cctggcattg aaacacacat atgaagtgta
     1321 cttcggatat gaccgtacgc tcaggaagta cggcagattc cgtcaaaaat tacctgtaaa
     1381 gacagacatc agaaaaaaca gtgctcgccg cagaccggtg accgggcgaa gcccgcgaac
     1441 cggcgaggaa gcgcaatgta tctgtagtta cagggagcag gtacgccctg aagccctttc
     1501 ataaaacggt tgtgtgcgct gtccttctgg aatgatttat ccacatatcc ggaggcccga
     1561 tccagtaaac agatccatga atgatcaaca aaggatccat taaagatccc catatcgctg
     1621 caaaccttgt cactcatggg ccgggaccac gatcacataa gctgtggcat gttactgata
     1681 aactgtaaca tgctaatgat aagctgtatt cagtaatcca tatactgaag taagttaatg
     1741 acataaacta tggtcagtat gccagactca gttgttaaat acaggctgca ggtttttctt
     1801 cagtcagtta gcggagttct gacacacgat tttctgttta ttcttttact gtccacaggc
     1861 tggaggcttt ctggaaaacg aaaattcaga catcaaaaaa ctgttcggcg aggtggataa
     1921 gtcgtccggt gagctggtga cactgacacc aaacaataac aacaccgtac aacctgtggc
     1981 gctgatgcgt ctgggcgtct ttgtaccgac ccttaagtca ctgaagaaca gtaaaaaaaa
     2041 tacactgtca cgcaccgatg ccacggaaga actgacacgt ctttccctgg cccgtgcaga
     2101 aggattcgat aaggttgaga tcaccggccc ccggctggat atggataacg atttcaagac
     2161 ctgggtgggg atcattcatt cctttgcccg ccataacgtg attggtgaca aagttgaact
     2221 gccttttgtt gagtttgcaa aactgtgtgg tataccttca agccagtcat cccgcaggct
     2281 gcgtgagcgc atcagccctt ccctgaagcg cattgccggt accgtgatct cgttttcccg
     2341 taccgatgag aagcacaccc gggaatacat cacccatctg gtacagtcag cctactacga
     2401 tactgagcgg gatattgtgc agttacaggc cgatccccgc ctgtttgaac tgtaccagtt
     2461 tgacagaaaa gtccttctcc agcttaaggc gattaatgcc ctgaagcgac gggagtccgc
     2521 ccaggcactc tacaccttta tagagagcct gccccgggat ccggcaccga tatcgctggc
     2581 gcggctgcgt gcacgcctca atctgaagtc tcctgtattt tcccagaacc agacggtcag
     2641 acgggcaatg gagcagctgc gcgagattgg atatcttgat tacacggaga tccagcgggg
     2701 gcggacaaaa ctcttctgca ttcactaccg gcgtccccgg ttaaaagcac cgaatgatga
     2761 gagtaaggaa aatccgttgc caccttcacc tgcggaaaaa gtcagtccgg agatggcgga
     2821 gaagcttgcc ctgcttgaaa aactgggcat cacgctggat gacctggaaa aactcttcaa
     2881 atcccgctga acataaactg tagtcagtga agagtgctcc tttactgact acagcttata
     2941 ttatcaggtg cagtgagtgg tctgctcact gcagtttata ttcagtttcc tgcagtgctg
     3001 cctgtggctg agctgccatc tgcctgtccc ttacgtgagt caccccgtaa cctgatgctg
     3061 aggcattgct cccttcataa aacatggttt actcactaca gcttatctac ctgctccagc
     3121 ttatgttatg tctgttctgc tgaccactgc ttatataagg aagtgccgtt acttcagggg
     3181 aggcagtgta cgcaggatct ccgcagcatc ccgaccgtca cctgtgaaag gcactgccag
     3241 cgtggcagcc atatccagtg caaacactct ggtataaacc tccatagaac gtggatccct
     3301 gtgaccagcc agtgcctgga tgactttccg gggctggcgg tgatagagca tgtgcatgat
     3361 atagctgtgc cggaaggtat gtggtgtgac cggaatcgaa aagtgtactc cgtcagcttc
     3421 ggcccgtctg acagcctgct tcagccagtt gcgcatggtt tcgtcggtca cggcccataa
     3481 tggttcacga cgacggggcc gggtggtgat catccagctt tccatctgcc tgacatagct
     3541 tatatccgtc agcggaacca ggcggacttc atcttttggc gggcgtccgc gtcgcgcacg
     3601 cactttttcg gacaggatcc gcacaaacgg tcttactcca tccaggtcaa atgattccgg
     3661 tgtcagcatc cgggcttcgc caatacgcat tccggtattc cagagggtgg cgaacagcat
     3721 atggtgacgc tgatccggca tatagaaaag aagggcactc acttccgggg ccagcaggta
     3781 ggccggtgtg gcccctgtgg aagtggccat tcttctcagg gagagcgctg ttgcaaaatc
     3841 cacccccggc gcaatgggta acagggagac gcgttctggt gaattctgca ggggaatgac
     3901 attgttcatg agttattttt cacgtagcac acgcactgtt tatggataca ggtattctga
     3961 cctgaacgcc cggatccttc cacgacaata aatcgcagag cgcggatcct tttgtttgct
     4021 tatcacgcat tattgcgggt aaaaccaaat acgtaaaggc gcgttttgcc gcagggatcc
     4081 tgtcttaagc cagtcatggt gcgggctgag gactggtttg ccgtttttgg gagaattggg
     4141 gtaatgccag gaacaggcag atacggagaa aaatcctgca gataaccttc tacgacaact
     4201 gaaggtgtct gacgccatat tcttagctac ttctcagttt taaggc
//
LOCUS       DAPHCV010000222         4226 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.225, whole genome shotgun sequence.
ACCESSION   DAPHCV010000222 DAPHCV010000000
VERSION     DAPHCV010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.225"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..242
                     /locus_tag="RH033_004612"
     CDS             <1..242
                     /locus_tag="RH033_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDV2993678.1"
                     /translation="LYRAEDRAERLALLACLLAVIPALNLASSDAAAVAGLVMTVLTV
                     GLVSCAGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            301..1161
                     /locus_tag="RH033_004613"
     CDS             301..1161
                     /locus_tag="RH033_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV2993679.1"
                     /translation="MKITDHKLPEGIALTFRVPEGNIKHPLILLCHGFCGIRNVLLPS
                     FANAFTEAGFATITFDYRGFGESEGERGRLVPAMQTEDIISVINWAEKQVCIDNQRIG
                     LWGTSLGGCHVFNAAAQDKRVKSIVSQLAFADGEVLVTGEMNELEKASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDNESKVFFEKVKGQYPEMDIKIPFLTVMETLQYKPAESAA
                     KVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGAFFERVAA
                     VQTQWFKKHL"
     gene            1264..1821
                     /locus_tag="RH033_004614"
     CDS             1264..1821
                     /locus_tag="RH033_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HDV2993680.1"
                     /translation="MTEQKRPVLTLKRKTEGETPIRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLTLPSLDEAVNTLKPWWPGLFDGNTPRLLACGIRE
                     VLLDEVSQRNIPLSHKKLRRALKAITRSESYLGAMKAGACRYDTEGYVTEHITQEEEQ
                     YAQARLEKVRRQNRIKDELRAILAE"
     gene            1976..2179
                     /locus_tag="RH033_004615"
     CDS             1976..2179
                     /locus_tag="RH033_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993681.1"
                     /translation="MAVSSGLSEHSSIYRYQREGRHAVRQEIRGFFREAAEVWQNAAR
                     LAPCEAWRDFALKRASACRCKCR"
     gene            complement(2636..3813)
                     /locus_tag="RH033_004616"
     CDS             complement(join(2636..3555,3555..3813))
                     /locus_tag="RH033_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611881.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDV2993682.1"
                     /translation="MNKKTKRTFTPEFRLECAQLIVDKGYSYRQASEAMNVGSTTLES
                     WVRQLRRERQGIAPSATPITPEQQRIRELEKQVRRLEEQNTIFKKGYRALDVRLTERF
                     SIVSRLSDSHSVVSLCSALGIHRSSYRYWRKRRDTVNSARVRLCSEIRRAWNQSRGSA
                     GARTLAEMLTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNACQEHTCLPNLLERQF
                     AVPEPDRVWCGDITYIWAGNRWCYLAVVMDLFARRVIGWSLSANADTALISSALRMAY
                     EVRGQPRDVMFHSDQGSQYTGLKYQQLIWRYRIKQSVSRRGNCWDNSPMERFFRSLKT
                     EWVPTDGYVGKDEARQQISGYILNYYNSVRPHHYNGGLTPEESENRYRFYCKTVANIT
                     "
BASE COUNT         1103 a          953 c         1103 g         1067 t
ORIGIN      
        1 gactgtaccg ggcggaagac agagcggaac gtctggcgct gcttgcatgc ctgctggccg
       61 ttattccggc acttaacctt gcctccagtg atgcagcggc ggtggccggt cttgtaatga
      121 cggtgctgac cgttggtctg gtgtcgtgtg caggaaaatc attaccccgt ttctggcccg
      181 gggatttttt cccggtattc tacgcctgtc atctggctgt gctgggcgtt ctggcgttgt
      241 gactggtatg atactgacgc tgattctgat tattaataaa caacataaca caggatattt
      301 atgaaaatta cggatcacaa actccctgaa ggtattgccc tgacatttcg tgtcccggaa
      361 ggaaatataa aacatccttt gattctctta tgccatggat tttgcggtat ccggaatgtt
      421 ctgctgcctt cctttgcgaa tgcgtttaca gaagccgggt ttgcaacgat tacttttgat
      481 tatcgtggat tcggtgagag tgaaggggaa cgaggtcgtc tggttccggc aatgcagacc
      541 gaagatatta tttcagttat caactgggca gaaaagcagg tatgtattga taatcaacgt
      601 ataggattat ggggaacatc gctgggtggg tgtcatgtat tcaatgcagc tgcgcaggat
      661 aaaagagtga aaagtattgt cagccagctg gcttttgctg acggtgaggt tctggttacc
      721 ggagaaatga atgaactgga gaaagcttct tttctgtcca cgctgaataa aatggcagaa
      781 aagaagaaaa ataccgggaa agagatgttt gtgggggtta cccgggtact gagtgataat
      841 gaatcaaagg tattcttcga gaaggtaaaa ggtcagtatc ctgaaatgga tataaaaata
      901 cctttcctta cggttatgga gacgttgcaa tataaaccgg cagagagcgc agccaaagta
      961 cagtgcccag ttttggttgt tattgccggc caggatagtg ttaatccacc agaacaggga
     1021 agagccttat atgatgctgt tgcttctggt actaaggaat tatacgagga agctgacgca
     1081 tgccattatg atatttatga aggcgcgttt tttgaacgtg tggcggcagt acagacgcaa
     1141 tggtttaaaa aacatcttta attgtcggta ttaatatgtc agaggtcgtt gaggtcagct
     1201 taatatcata gtgagtagtt tcaacgacct ggaggacata ttttcacaaa ggtcagggca
     1261 atcatgacag agcagaaacg accggtactg acactgaaac ggaaaacgga aggggaaaca
     1321 ccgatccgga gccggaaaac catcatcaat gtcaccacgc caccaaaatg gaaggtgaaa
     1381 aagcagaaac tggcggagaa ggctgcccgg gaagcagagc tggcggcgaa gaaagcacag
     1441 gccagacagg cgctgtccat ttatctgacg ctgccctcgc tggatgaggc ggtgaacact
     1501 ctgaaaccct ggtggccggg attgtttgac ggtaacacgc cccggcttct tgcctgtggt
     1561 atccgggaag ttttactgga tgaggtgtct cagcggaata tcccgctctc tcataaaaaa
     1621 ctgcgcaggg cactgaaggc catcacccgt tcagaaagct atcttggcgc catgaaagcc
     1681 ggtgcctgcc gctatgatac agaaggctat gtgacagaac atatcacaca ggaagaagaa
     1741 cagtatgcgc aggcgcgcct ggaaaaggtg cgcagacaga accggatcaa ggatgagctg
     1801 cgggcgatcc tggcagaata ataaaagcgc cctctgtatg acagagggca acagcgaaaa
     1861 aaatcgggaa aacagacatc cagagttttg atggagaaaa acatccaacc gttcagggat
     1921 gtttcataat gtttctgaca taatccgggt gtcgggtcaa gaggagggat aaaaaatggc
     1981 tgtatccagt ggattatctg aacacagcag tatttatcgc tatcagcgcg aggggcgtca
     2041 tgccgtccgt caggagattc gtggcttttt cagggaagcg gcagaggtct ggcagaatgc
     2101 ggcccggctg gcaccctgtg aagcatggcg ggattttgct ctgaagaggg catctgcatg
     2161 tcggtgtaag tgccgataaa aataaccccc gtcgcggagc tccgggggat cagacttata
     2221 tggcagatcg ttattattat tatagtggtg taagaaggag ttgccctgtc tttcaaacgg
     2281 acatcacaga attaacatat ttcacatccc gggtaagcca gcagggataa aaaatgtgat
     2341 gaatttcaag aagcaggagg tgacagtggc atgcataaca gtagggttat ccggtgaatc
     2401 tgacagacag ccgggctgta ttcgcggcag gataaagaaa aaccgggaga tgactctccc
     2461 ggtccgtctt atcgctaatc ccctgagcag ggcatgtatg acttatctat gaccgttata
     2521 ttcagcgcca catccggtag cctgcgatat aacacagtta tcctgtcagc aagagcaggt
     2581 atgatgaata cacggagatt aaaaatataa tctccgtcac atgttactgt agtggtcaag
     2641 taatattggc cacggtttta cagtaaaaac ggtatctgtt ctctgattct tccggcgtca
     2701 acccaccgtt gtaatggtga ggcctgacgc tattgtaata attcaggata taaccgctaa
     2761 tttgttgccg ggcctcgtcc ttgcctacgt aaccatccgt cggcacccat tctgttttca
     2821 gactgcggaa gaagcgttcc ataggactgt tatcccagca gtttccccgt cggctgacac
     2881 tttgctttat cctgtaacgc cagataagct gttgatattt cagtcctgta tactgacttc
     2941 cctggtcgct atggaacatg acgtcccgcg gctgaccacg cacctcatac gccatccgca
     3001 aggcactgct tatcagtgca gtatcggcat tcgctgacag gctccagccg ataaccctgc
     3061 gggcaaaaag atccatgacg accgccagat agcaccagcg atttcctgcc cagatatacg
     3121 taatatctcc gcaccatacc ctgtctggct ccggtacagc gaactgacgc tcgagcagat
     3181 tcggcaaaca cgtatgctcc tgacaggcat ttttgtattg atgttttccg ggctgacaac
     3241 tgctcaggtt caggtatttc atcagccgtc cggcacgata acggctcatc gggacgccgt
     3301 tttgggtcag catttcagcc agcgtgcgcg cgcctgcaga gccccggctc tggttccatg
     3361 cccggcgtat ttcgctgcac aacctgacgc gagccgaatt aaccgtatcg cgtcgttttc
     3421 gccagtaccg gtaactgctg cggtgtattc ccagtgcgga acagaggctg acaactgagt
     3481 ggctgtcact cagtctggaa actatcgaaa accgttcagt gagtcggaca tcaagagcgc
     3541 ggtagccttt tttaatatcg tattttgttc ctccagtcgg cgaacctgct tttccaactc
     3601 gcgaatacgt tgctgttcag gggtaatggg tgttgcagag ggcgcaatac cttggcgctc
     3661 tcgcctgagc tgacgtaccc agctctcaag cgtggtagaa ccgacattca tcgcctcact
     3721 ggcttgtcga tacgaatagc ccttatcaac aatcagctgt gcacattcca gcctgaactc
     3781 aggggtgaaa gtacgtttgg ttttcttgtt cataagtcac ctgttttgtg ttgaggtgag
     3841 aatatcacct ttaatcaggt ggccaaattt actgtgccac tacataccca cggaaaccgg
     3901 taacgccttc acgggaaata tgtgacagaa aaagcagcct ccgttactcc aggtatcagg
     3961 aggctgaata atgcatgaga cgttgtttct tatgtgtctg gtcaggctgg ctgaccagcg
     4021 tttttagtaa cacatagcca tatgtgtatt aagtcacctc atcttatttt gttgaattga
     4081 tcacaactca acggtttatt ttttgcgtga tacgtgagcc attattcatc ccggcatgga
     4141 attcatgcaa tcacgaaaag taatcagtga cggagagtaa aatgaagaaa tatataccag
     4201 tggtattatt tgttttttca tgtcag
//
LOCUS       DAPHCV010000223         4225 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.226, whole genome shotgun sequence.
ACCESSION   DAPHCV010000223 DAPHCV010000000
VERSION     DAPHCV010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.226"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..836
                     /gene="traO"
                     /locus_tag="RH033_004617"
     CDS             <1..836
                     /gene="traO"
                     /locus_tag="RH033_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HDV2993683.1"
                     /translation="GAPGISSHASPPGTGTSPQPRLQEASLATPAARTGIQLIPALTR
                     VPAFIDTEVDSDNAVSRVVASVPVGPWAGAVLYSQDARLAGKGMDIHFDRMMWRGMFL
                     KVNAYALNEHTGMSSVASDVNHRWFKHIVLPSVLGGVGSVGSLYKDANTQVIQGNYGS
                     ITGRVGMPDGEALAGVIAGGMAERGSQLLTRQAESEPYRQVTVSRGEVVSILFVEPVM
                     SNDISPPSSGTAPSVPPPSGRPLTQEEAERQASDRIQAAVMRKQNEMRQRYGIQQESH
                     P"
     gene            833..1546
                     /gene="traP"
                     /locus_tag="RH033_004618"
     CDS             833..1546
                     /gene="traP"
                     /locus_tag="RH033_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HDV2993684.1"
                     /translation="MKNEHEDPGSFGETTTPASGKSFLKRQYMGYSLPVWAGCVLIIL
                     AAVWYKFLRTPSEPPADAGFAVTPPAAESWAEPMPMTETGSGNTMSAPPVSPQADMQQ
                     LARDLKAELDRRDEEIRRTLTMLKESITTLGDTIRKDEAWAQETRQLLDETRKQVHAL
                     QMQGPLQIPTGNNGQSTKTPPSRLQGMQIMSVEKGMAWIRWQGSAWAVREGDRLGNIT
                     ITRIEPSSRSVITSAGTLR"
     gene            1546..2073
                     /gene="traQ"
                     /locus_tag="RH033_004619"
     CDS             1546..2073
                     /gene="traQ"
                     /locus_tag="RH033_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HDV2993685.1"
                     /translation="MSIDALTAIENFASGIFSAGMEFLFTWGELLGIIGLIGHLMRAR
                     AEGRHSMGPGKFIAGIVICGMLVALPSFINAGGTQMGFRADSFGPIAYVQPTTFGAAA
                     GAANAMLSLVKLAGVGFAMNGISIWRKSLLDGHTALSSSESISKGNVKFVAGVLLVFI
                     DRVIDASLASLGLAF"
     gene            2122..2520
                     /locus_tag="RH033_004620"
     CDS             2122..2520
                     /locus_tag="RH033_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HDV2993686.1"
                     /translation="MSRFRAMAARLCAAFIYVQLCLVACRDRLIRLLLLLPVILLPHT
                     AMANTLFDMIKTVGDGAKGSSSSFVAIGQVIGLVLFIGGLLGLKQIGKKPGAGLACLL
                     SILVGLCLAVVPELMGSGQKTLGLSPVSIS"
     gene            2577..2828
                     /locus_tag="RH033_004621"
     CDS             2577..2828
                     /locus_tag="RH033_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003665769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraS"
                     /protein_id="HDV2993687.1"
                     /translation="MSCELPPFTLLRTEHGNVIARDNRDNQEFVFAECYSTAIAALIL
                     AAVTLYTERKTYEQNTATHRPGPGTLAFPAPATGHGIQR"
     gene            2743..3561
                     /locus_tag="RH033_004622"
     CDS             2743..3561
                     /locus_tag="RH033_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraT"
                     /protein_id="HDV2993688.1"
                     /translation="MNKTPPLIAPVRERWLFPLPQPDTVFSGESLLPPPVLSTPGRCP
                     CCRRTVTHRFILEDSWPLQQMADTCRDTVVLLEKNLTRVMRQKKHPVPENADEKKKHT
                     RTLQDAERSLAQARLSARRLALRHVEKSQIVTTDALSENESELLQPEGPPFHLCAFCH
                     AWHCLNGYAAAQGVMVWLPDLHPASVVALNARALKEIFSDERKRVRQGRAVLNALVQN
                     RLAVEEKFRTWRPADFADALRRWPPAQRKTLREKMDGVALILLPDSFPDKKYVM"
     gene            3620..3979
                     /locus_tag="RH033_004623"
     CDS             3620..3979
                     /locus_tag="RH033_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993689.1"
                     /translation="MDTHSAGYQFTADSFRRFIIRTLTDNIPPDGDEKEIARFILRHP
                     AFNGGWLLFLAAEIHERSRTHKSITVTVSVDNYLSWMDIAVSGLPELPDMPFCDFFIR
                     RQQFIYPGVPPTKSGEE"
     gene            3979..>4225
                     /locus_tag="RH033_004624"
     CDS             3979..>4225
                     /locus_tag="RH033_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993690.1"
                     /translation="MKIHRISPETLITLIHAHLAGKTDSTAKEEHRLLRRFLRDDDGR
                     LAGVLLNIAGILQFNRELSARHNYPATPLTEFSLRKRG"
BASE COUNT          968 a         1230 c         1147 g          880 t
ORIGIN      
        1 ctggtgctcc cgggataagc tcacacgcct caccacccgg taccggcaca tcaccacaac
       61 cacgtctgca ggaggccagt ctggcaaccc cggcagctcg tacagggata cagctgatac
      121 cggcactgac ccgcgtacct gccttcattg atacggaagt ggactctgac aatgctgtct
      181 cccgcgtggt ggcctccgta cctgtcggcc catgggccgg ggccgtcctc tacagccagg
      241 acgccagact cgccggcaag ggaatggata tccactttga ccggatgatg tggcggggca
      301 tgtttctgaa agtcaatgca tatgccctga atgaacatac cggcatgtca tccgtggcca
      361 gcgacgtgaa ccaccgctgg tttaagcata tcgttctgcc gtctgtgctc ggtggggtgg
      421 gcagtgtcgg aagtctttac aaagacgcca acacacaggt cattcaggga aactacggca
      481 gcattaccgg cagggtggga atgcctgatg gcgaggcgct ggctggcgtc attgcaggcg
      541 gcatggccga acggggcagt cagttgctga cccgccaggc ggagagcgaa ccttaccggc
      601 aggtgacagt gtcacgcgga gaggttgtct ccattctgtt tgttgagccg gtcatgagca
      661 acgatatcag tcccccctcc tccggcactg ccccttctgt gccaccgccc tccggacgcc
      721 cgctcaccca ggaagaagca gagcgtcagg cgtctgacag gatccaggca gctgtcatgc
      781 gtaaacagaa cgaaatgcgg caacgttacg gaatacaaca ggaaagtcat ccatgaaaaa
      841 cgaacatgaa gatcccggca gttttggcga aacaacgaca ccggcatccg gcaaatcctt
      901 cctgaaacgc cagtatatgg gatacagcct gccagtctgg gccggctgtg tcctcatcat
      961 cctggccgca gtctggtaca agtttctgcg gacaccctca gagccccctg cagatgcagg
     1021 cttcgctgtc acaccgcccg cagccgaaag ctgggcagag cccatgccca tgacggagac
     1081 cgggagcgga aacaccatgt ccgcgcctcc tgtctcccca caggcagaca tgcagcagct
     1141 tgcccgtgac ctgaaagcag agctggacag gcgggatgag gaaatccgac gtaccctaac
     1201 catgctgaaa gagagcatca caacccttgg cgacaccata cggaaagatg aggcctgggc
     1261 acaggaaacc cgtcagttgc ttgatgaaac ccgtaagcag gtgcatgccc tgcagatgca
     1321 gggaccactg caaatcccga ccgggaataa cggccagtca acgaaaaccc cgccttcccg
     1381 cctgcagggc atgcaaatca tgtcggtgga aaaaggaatg gcctggatcc gctggcaggg
     1441 tagcgcctgg gcagtacggg aaggtgacag gctggggaat atcaccatca cccggattga
     1501 accgtccagc cgctcagtca tcacctctgc cggtacatta cgatgatgag cattgatgcc
     1561 ctgacagcca tagagaactt tgccagcggt atattcagtg caggcatgga gtttctcttc
     1621 acctggggag agctgctggg gattatcggc ctgattggtc atctgatgcg tgcccgggct
     1681 gaagggcgtc acagtatggg accgggaaaa tttatcgccg gtatcgtcat ctgcggcatg
     1741 ctggtcgccc tgccatcctt catcaatgcc gggggcaccc agatgggatt cagggcagat
     1801 tccttcggcc ctatcgctta tgtacaacca accacctttg gtgccgctgc cggtgccgcc
     1861 aatgccatgc tctcactggt aaaactggcc ggagtggggt ttgccatgaa cggcatttcc
     1921 atctggcgta aatccctgct ggacgggcat accgccctga gcagcagtga gtccatcagc
     1981 aaaggaaacg tcaagtttgt tgccggcgtt ctgctggttt ttatcgatcg cgtcatcgat
     2041 gcctctctgg cgagtcttgg tctcgccttc tgatccacat tcattttcag tcattcagtc
     2101 tgttagttaa ggagacacta tatgtcacga ttccgtgcca tggcggcgcg tctgtgcgct
     2161 gcatttattt atgttcagtt atgcctggtg gcctgccgtg atcggctgat acgcctgtta
     2221 cttctgcttc ccgtgatact gctgccccac acagcgatgg caaacaccct attcgatatg
     2281 atcaaaaccg ttggcgatgg tgcaaaaggc tccagcagtt catttgtggc tatcggtcag
     2341 gttattggcc tggtgctctt tatcggcggc ctgcttggcc tcaaacaaat cggtaaaaaa
     2401 cccggtgccg ggctggcctg cctgctgtca attctggtcg gcctctgcct tgccgtcgta
     2461 ccggaactga tgggtagcgg acagaaaacg ctcggtcttt ctccggtttc aatcagttaa
     2521 tccgcaaagg caccgggaaa ccggtgccgt cttcacacaa tgaggaggac cagaccatgt
     2581 cctgtgagct gccccctttc accctgctca gaacagagca cggcaacgtg attgcccgcg
     2641 acaaccggga taatcaggag tttgtgtttg cagaatgtta ttccaccgcc atcgcagcac
     2701 tgattctggc tgccgtcacc ctgtatacag agagaaaaac gtatgaacaa aacaccgcca
     2761 ctcatcgccc cggtccggga acgctggctt ttcccgctcc cgcaaccgga cacggtattc
     2821 agcggtgaat ccctgctgcc accaccagtg ctctcaacac ccggacgctg tccctgctgt
     2881 cgcaggaccg tcactcaccg cttcattctg gaggattcat ggccattaca gcagatggct
     2941 gacacctgcc gggatacggt cgtcctgctg gaaaaaaacc tcacccgggt gatgcgccag
     3001 aaaaaacatc cggtaccgga aaatgctgac gaaaaaaaga aacacacccg aaccctgcag
     3061 gatgcggagc ggtccctggc acaggcccgc ctgtcggcca ggcgactggc cctgcgtcat
     3121 gtggaaaagt cacaaatcgt cacaacagat gcactcagcg aaaatgaaag tgaactgctg
     3181 cagccggaag gcccgccctt tcatctctgt gccttctgcc acgcctggca ctgcctgaac
     3241 ggctacgctg ccgcacaggg ggtgatggtc tggctccctg atttgcatcc ggcctcggta
     3301 gttgctctca atgcacgggc actgaaggaa attttcagcg atgagcgtaa gcgagtcaga
     3361 cagggacggg ccgtactgaa tgccctggta cagaaccgcc tggccgttga ggagaaattt
     3421 cgcacctggc gtccggcgga ttttgctgat gcactgcgcc gctggccgcc ggcacagcgt
     3481 aaaacgttga gggagaaaat ggacggggtg gcgctcattc tgctgccgga ttcgtttcct
     3541 gacaaaaagt acgtcatgtg acacacaccg gtaccgatga cattctgccg gcacacattc
     3601 aacttacaga ggaacaaaaa tggatacaca ctctgccgga tatcagttca ctgcagattc
     3661 attccggcgt tttattatac ggacgctgac tgacaatatt ccgcctgacg gagatgaaaa
     3721 agaaattgcc cggttcattc tccgccaccc ggcatttaac ggcggctggc tgctctttct
     3781 ggctgcagaa attcatgaaa gatcccgtac ccataaaagc atcaccgtca cagtgagcgt
     3841 cgataactat ctctcatgga tggatattgc cgtatccggt ctcccggaat taccggatat
     3901 gcccttctgt gattttttca ttcgccgcca gcagtttatc tatccgggtg tgcccccgac
     3961 aaaatcagga gaagaatgat gaaaattcac cgcatatccc cggagacgct tataacactc
     4021 atccacgcac acctggccgg aaaaactgac agtaccgcca aagaagagca ccggctactc
     4081 cgtcgtttcc tgcgggatga tgacggcagg ctggccggcg tactgctgaa tattgccgga
     4141 atacttcagt tcaacaggga attaagtgcg cggcataatt atccggccac acccctgact
     4201 gaattttcat taaggaaacg gggaa
//
LOCUS       DAPHCV010000224         4220 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.227, whole genome shotgun sequence.
ACCESSION   DAPHCV010000224 DAPHCV010000000
VERSION     DAPHCV010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.227"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            116..4057
                     /gene="vat"
                     /locus_tag="RH033_004625"
                     /pseudo
     CDS             116..4057
                     /gene="vat"
                     /locus_tag="RH033_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021534952.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vacuolating autotransporter toxin Vat"
BASE COUNT         1159 a          963 c         1081 g         1017 t
ORIGIN      
        1 ggtatgtaaa gggagtaccc gcagaggaaa aagactttca gtacttacct ctctggcact
       61 atctgcatta ctcccaaccg ttgctggtgc atcaacggtt ggtggcaaca atccttacca
      121 gacataccgc gactttgcag aaaacaaagg gcagtttcag gctggcgcaa caaacattcc
      181 tatttttaat aataaagggg aattagtagg acatcttgat aaagcgccca tggttgattt
      241 tagcagtgtg aatgtaagct caaatcccgg cgttgcaaca ttaattaacc cgcaatatat
      301 agccagtgta aaacataata aaggatatca gagcgtcagc ttcggtgatg gtcagaacag
      361 ttaccatatt gtggatcgta atgaacacag ttcatctgat ctccacacac caagacttga
      421 taagctcgta actgaggttg ctccggctac cgtaaccagc tcatcaacag ctgatatatt
      481 gaacccttca aaatactcgg cattctacag ggctggttcg ggaagtcagt atattcagga
      541 tagtcagggt aagcgacatt gggtaacagg tgggtatggt tatctgacag gaggaatact
      601 cccgacatca ttcttttatc acggctcaga cggcattcag ctgtatatgg ggggcaacat
      661 acatgatcat agcatcctgc cctcttttgg agaggccggc gacagtggtt ctccattatt
      721 tggctggaat acggccaaag ggcagtggga actggtcggt gtttactcgg gagtaggagg
      781 ggggaccaat ttgatatatt ctcttattcc tcagagtttt ctctcacaga tctattcaga
      841 ggataatgac gctcccgtct tttttaatgc ctcatccggc gcccccctgc aatggaaatt
      901 tgacagcagc accggcactg gctctctgaa acagggttcc gatgaatatg ccatgcacgg
      961 gcaaaaaggt tctgacctga acgcaggtaa aaatctgaca ttcctgggac ataatggtca
     1021 gattgacctg gaaaactctg tcacgcaggg tgccggttca ctgacattta ctgatgacta
     1081 cactgtcacc acttcaaacg gaagtacctg gaccggggcc ggtattattg tggacaagga
     1141 tgcctccgta aactggcagg ttaatggtgt gaaaggtgac aacctgcata aaatcggcga
     1201 aggaaccctg gttgtacagg gaaccggtgt taatgagggc ggcctgaaag tcggggatgg
     1261 gaccgttgtc ctcaatcagc aggctgacag ttcaggacac gttcaggcat tcagtagcgt
     1321 gaatattgcc agcggccgcc cgacagtcgt gctggcagac aaccagcagg ttaatccgga
     1381 caatatatcc tggggctacc gggggggggt tctggatgtt aacgggaatg acctgacatt
     1441 tcataagctg aatgccgccg attatggcgc aactctcggt aacagcagtg ataaaacggc
     1501 taatatcact ctggattatc agacgcgtcc ggcagacgta aaagttaatg aatggtcatc
     1561 atcaaacagg ggaacagtag gttcattata tatttataat aatccctata ctcataccgt
     1621 cgattatttt atcctgaaaa caagtagtta tggctggttc cctaccggtc aggtcagtaa
     1681 cgagcactgg gaatatgtcg gacatgacca gaacagtgca caggcactgc ttgcaaacag
     1741 aattaataat aaagggtatc tgtatcatgg caagttgctg ggaaatatta atttctcaaa
     1801 taaagcaacc ccgggtacaa ccggcgcatt ggttatggac ggctcagcga atatgtccgg
     1861 tacatttact caggaaaacg gtcgtctgac cattcagggc cacccggtta tccatgcttc
     1921 aacgtctcag agtattgcaa atacagtctc gtctctgggc gacaattccg ttctgacaca
     1981 gcccacctca tttacacagg atgactggga gaacaggacg ttcagctttg gttcgctcgt
     2041 gttaaaagat acagactttg gtctgggccg caatgccaca ctgaacacaa ccatccaggc
     2101 agataactcc agcgtcacgc tgggcgacag tcgggtattt atcgacaaaa aagatggcca
     2161 gggaacagca tttacccttg aagaaggcac atctgttgca actaaagatg cagataaaag
     2221 cgtcttcaac ggcaccgtca acctggataa tcagtcagtg ctgaatatca atgagatatt
     2281 caatggcgga atacaggcga acaacagtac cgtgaatatc tcctcagaca gtgccgttct
     2341 ggagaactca acgctgacca gtaccgccct gaatctgaac aagggagcaa atgttctggc
     2401 cagtcagagt tttgtttctg acggtccggt gaatatttct gatgccaccc tgagtctgaa
     2461 cagccgtcct gatgaggtat ctcacacact tttacctgta tacgattatg ccggttcatg
     2521 gaacctgaag ggagacgatg cccgcctgaa cgtggggccg tacagtatgt tgtcaggtaa
     2581 tatcaatgtt caggataaag ggactgtcac cctcggaggg gaaggggaac tgagtcctga
     2641 cctgactctt cagaatcaga tgttgtacag cctgtttaac gggtaccgca atacctggag
     2701 cgggagcctg aatgcaccgg atgccaccgt cagcatgaca gacacccagt ggtcgatgaa
     2761 cggaaactcc acggcaggaa atatgaaact taaccggaca atagtcggtt ttaacggggg
     2821 aacatcatcg ttcacgacac tgacaacaga taatctggac gcggttcagt cagcatttgt
     2881 catgcgtaca gaccttaaca aggcagacaa actggtgata aacaagtcgg caacaggtca
     2941 tgacaacagc atctgggtta acttcctgaa aaaaccctct gacaaggaca cgcttgatat
     3001 tccactggtc agcgcacctg aagcgacagc tgataatctg ttcagggcat caacacgggt
     3061 tgtgggattc agtgatgtca cccccaccct tagtgtcaga aaagaggacg ggaaaaaaga
     3121 gtgggtcctc gatggttacc aggttgcacg taacgacggc cagggtaagg ctgccgccac
     3181 attcatgcac atcagctata acaacttcat cactgaagtt aacaacctga acaaacgcat
     3241 gggcgatttg agggatatta acggcgaagc cggtacgtgg gtgcgtctgc tgaacggttc
     3301 cggctctgct gatggcggtt tcactgacca ctataccctg ctgcagatgg gggctgaccg
     3361 taagcacgaa ctgggaagta tggacctgtt taccggcgtg atggccacct acactgacac
     3421 agatgcgtca gcaggcctgt acagcggtaa aacaaaatca tggggtggtg gtttctatgc
     3481 cagtggtctg ttccggtccg gcgcttactt tgatttgatt gccaaatata ttcacaatga
     3541 aaacaaatat gacctgaact ttgccggagc tggtaaacag aacttccgca gccattcact
     3601 gtatgcaggt gcagaagtcg gataccgtta tcatctgaca gatacgacgt ttgttgaacc
     3661 tcaggcggaa ctggtctggg gaagactgca gggccaaaca tttaactgga acgacagtgg
     3721 aatggatgtc tcaatgcgtc gtaacagcgt taatcctctg gtaggcagaa ccggcgttgt
     3781 ttccggtaaa accttcagtg gtaaggactg gagtctgaca gcccgtgccg gcctgcatta
     3841 tgagttcgat ctgacggaca gtgctgacgt tcacctgaag gatgcagcgg gagaacatca
     3901 gattaatggc agaaaagacg gtcgtatgct ttacggtgtg gggttaaatg cccggtttgg
     3961 cgacaatacg cgtctggggc tggaagttga acgctctgca ttcggtaaat acaacacaga
     4021 tgatgcgata aacgctaata ttcgttattc attctgatgc tgtgcagcaa agggactatt
     4081 gctttgtccc tttgctgcga ttatctgatt acggtacagg ctggaactta gattttccgg
     4141 cgttcacgat acttcatgta acactcaggt tgagtaatct tcatgagttt tcttttgccg
     4201 tgtggtggaa ttatgaataa
//
LOCUS       DAPHCV010000225         4214 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.228, whole genome shotgun sequence.
ACCESSION   DAPHCV010000225 DAPHCV010000000
VERSION     DAPHCV010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.228"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            6..1055
                     /gene="yeeE"
                     /locus_tag="RH033_004626"
     CDS             6..1055
                     /gene="yeeE"
                     /locus_tag="RH033_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001647169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HDV2993691.1"
                     /translation="MILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYALLI
                     AISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYIFGLGIVLEGGCATGTWYRAGEG
                     LIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVTVLLVI
                     TLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLSEAT
                     GRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKGSREFRVRAADA
                     QTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWVGLVFMILGVWTASWLVY
                     VRPQRKARLATAAAN"
     gene            1069..1296
                     /locus_tag="RH033_004627"
     CDS             1069..1296
                     /locus_tag="RH033_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDV2993692.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMTSGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWNITVQKA"
     gene            complement(1339..2766)
                     /gene="sbcB"
                     /locus_tag="RH033_004628"
     CDS             complement(1339..2766)
                     /gene="sbcB"
                     /locus_tag="RH033_004628"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HDV2993693.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            2975..4141
                     /gene="dacD"
                     /locus_tag="RH033_004629"
     CDS             2975..4141
                     /gene="dacD"
                     /locus_tag="RH033_004629"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HDV2993694.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNIDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
BASE COUNT          994 a          974 c         1125 g         1121 t
ORIGIN      
        1 tttcaatgat attaagcggg ctaatttgtg gtgctctgct gggatttgtc atgcagcgtg
       61 ggcgtttttg cctgactggt ggttttcgcg atatgtatat cgcgaaaaat aatcgcatgt
      121 tttatgccct gctgattgca atttcggtac aaagcgtggg ggtttttgcg ttaattcagg
      181 cgggcctact gacttacgaa gccggggcgt tcccgtggct tggtactgtt ataggtgggt
      241 atatcttcgg gctgggaatt gttctggagg gtggatgtgc caccgggacc tggtatcgcg
      301 cgggggaggg attgatcggc agttggatcg cgcttttcac ttatatggtg atgagtgcgg
      361 tgatgcgttc tccacatgcc agtggtttaa atcaaacctt gcagcactac agtactgaac
      421 ataactctat tgctgagact tttaatttgt ctgtgtggcc gttggttacc gttttgctgg
      481 tgataacgct ctgggtggtg atgaaagagt tgaagaagcc aaaactcaaa gtcgcgacct
      541 taccgccgcg ccgaaccggg atcgctcata ttctgtttga aaaacgctgg catccctttg
      601 tcactgctgt actcatcggt ttgattgcgc ttttagcctg gcccctgagt gaagcaaccg
      661 gacgcatgtt tgggttagga atcacttccc caacggccaa tattctgcaa tttctggtcg
      721 cgggtgacgt gaaatacatt aactggggcg ttttcctggt gttagggatc ttcgtggggt
      781 catttattgc agccaaaggg agccgtgagt tccgcgttcg cgcagctgat gcacaaacaa
      841 cattacgtag cgggttaggt ggtgtactga tgggcttcgg tgccagtatt gcaggtggtt
      901 gctctatcgg taatggactg gttatgactg caatgatgac ctggcagggc tgggttggcc
      961 ttgtatttat gattctcggt gtctggactg cgtcctggct tgtgtatgtt cgaccgcagc
     1021 gtaaggcgcg actggcgact gctgcggcaa attaagtaag gtgtaaagat ggcgattaaa
     1081 aagctggatg tggtgacgca ggtctgtccg tttccactca tagaagcaaa agcagcactg
     1141 gcagaaatga ccagcggcga tgagttggta attgagtttg attgcaccca ggcaacagaa
     1201 gcaatcccac aatgggcggc agaagaagga catgccatca ccgattatca gcagattggc
     1261 gatgctgcgt ggaacatcac cgttcaaaaa gcctgaccca acattgtcct ccgccgtatc
     1321 agcggcggag gcttcaaact agacaatctc ttccgcgtac tgccaaagcg cttttaacag
     1381 cgccactttt tctttatcgt cggcatactg ctgcgccagc atctgaatct cttctgcata
     1441 gccttgtaaa aactcgggtg tgaatacctg gcggcggtgc tccagccagc gttgctgctc
     1501 ggcataatcc agcgtccccg ggaagttgcg cgcccgataa ttgaacaaca gcttttcaat
     1561 ccgtttatcg acaaaagtga tatccagtgc cggtaaatta cgcggctcgg tttccagcac
     1621 aattttcatt gctgcacgat cggcgtcact gaaaaagccg ttataaagct gtgcatccac
     1681 gttatctgaa ggcgtaaacg gttcggcttc cgcgaatatc gccaccactt tttcgcgcac
     1741 ttgcggattt tcacgcagaa ttttcaggtt atcgaggcaa tgctgacgat taattcccag
     1801 tcggtcggca tcttccgggc gtagcgtatt cgcctgggcc agcaccggac atttattgat
     1861 gtgcaccagc ttaaccggaa cggcggcgtt atcgccaaga tcggtttttg cggtatataa
     1921 acgctcgcgc aatgtgtcgc tatccagttc cagtaatggc gaaatgtctc ctgccaaatc
     1981 caccataatt acggcattgc gattttcagg atgccacgcc agcggtgcca cccagctggt
     2041 gttgccgcgc catgccccaa acattccgga aacgtgcacc aggggtttca tctgcggaac
     2101 atcaatcaac gccatcagtt tgtgtttatt acgatgggta aagagataat caaacaggcg
     2161 tggctgacgc gtttttacca gctttgccat cgcaatagtg gcgtacacat cagccatcgc
     2221 atcgtgggcg ttgctatgtt caataccatt cgctttggtt aaatgctcaa ggcgaaagct
     2281 cggtagaccg tcatcatttt caggccagtt tattccttcc gggcgcaggg cataacaggc
     2341 acgcataaca tccagtaaat cccagcgcga gttatcatgc tgccagctcc aggcgtaagg
     2401 atcgtagaaa ttacgataaa aaacgttgcg tgtgacttcg tcgtcgaaac gcacattgtt
     2461 gtagcccaga atacaggtct tcggtacggt aaaaagcgag tgaatacggg cggcaaacgc
     2521 ggcttcgttt tctcctttcg cccgtgcttc ctgcggggta ataccggtaa ttaatactgc
     2581 tccaggctgg ggtaaatagt catcagcggg cttgcagtaa aagacttcgg gttcgccgat
     2641 gacattgaat tcgctatcgg tgcgaatggc tgcgaactgt gcagggcgat ctaacgcggg
     2701 gtgcgtgcca aaggtttcgt aatcgtgaaa caaaaaggta gattgttgct taccgtcatt
     2761 catcattagg ttaaatccgt tatttctgct gtctgccaga gtatcaaata tcaccgtgct
     2821 aatcagcttt agcgccacaa tttggcagcg agtggcaaca gatcatgtca gataaaaatg
     2881 agagggtagt cacattttct tgcactttat tccagccagt tcataagtat ttccgtaaaa
     2941 agaacagcta tttgaaactc ctgagggttt gctgttgaaa cgccgtctta ttattgctgc
     3001 ttctttgttc gtttttaact tatcgtctgg ttttgcggcg gaaaacattc ctttttcacc
     3061 tcagcctcca gagattcatg ccgggtcctg ggtactgatg gattacacca ccggacagat
     3121 cctcaccgcg ggtaatgagc atcaacagcg caatcccgcc agcctgacaa agctgatgac
     3181 gggctatgtc gtggatcgcg ctatcgatag tcatcgcatt acgccagacg atattgtcac
     3241 cgtggggcgc gatgcgtggg cgaaagataa tccggtgttt gtcggttctt cactgatgtt
     3301 tttgaaagag ggcgatcgcg tatcggtacg tgatttaagc cgtggtttaa ttgtggattc
     3361 cggaaatgac gcttgtgttg cattggctga ctatattgcc ggtgggcaac ggcagtttgt
     3421 tgaaatgatg aacaactatg ccgagaagct gcatctcaag gatacgcatt ttgaaacagt
     3481 gcatggcctg gatgcgccag gtcagcatag ctcggcttat gatttagctg tgctttctcg
     3541 tgctatcatc cacggcgagc ccgagtttta tcatatgtac agtgagaaaa gcctcacctg
     3601 gaacggtatc acccagcaaa accgtaacgg gttattgtgg gataaaacca tgaatattga
     3661 cggcctgaaa acgggtcata cttctggtgc cgggtttaat ctcattgctt cggctgtaga
     3721 tgggcagcgt cgtctcattg cagtggtaat gggggctgac agcgcaaaag gtcgtgagga
     3781 agaggcaaga aaattactgc gttgggggca acaaaacttt actacggtgc aaattttgca
     3841 ccgtgggaaa aaggtcggta cggaacgcat ctggtatggc gataaagaaa atatcgccct
     3901 gggaacagaa caagagttct ggatggtact accgaaagcc gaaattccac atatcaaagc
     3961 caaatatacc cttgatggta aagaactcac cgcgccaatt agcgcccatc agcgggtagg
     4021 ggaaattgaa ctttacgacc gtgataaaca ggtggcgcac tggccgctgg ttaccctgga
     4081 atctgtcggg gaaggcagca tgttttctcg ccttagtgat tatttccacc ataaggcctg
     4141 acctttcttt tgcagcagac tggcaggagt gcgagtctgc tcgcataatc aacactcatt
     4201 ccttgtggtt ttaa
//
LOCUS       DAPHCV010000226         4204 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.229, whole genome shotgun sequence.
ACCESSION   DAPHCV010000226 DAPHCV010000000
VERSION     DAPHCV010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.229"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            391..582
                     /locus_tag="RH033_004630"
     CDS             391..582
                     /locus_tag="RH033_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transport activator ProQ"
                     /protein_id="HDV2993695.1"
                     /translation="MVTLTINRKPQGIFSKSPATPLQNKTDTAYKMKAGDQIPQLNDK
                     QKPADKTPWRHDKTPEQKP"
     gene            651..899
                     /locus_tag="RH033_004631"
     CDS             651..899
                     /locus_tag="RH033_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transport activator ProQ"
                     /protein_id="HDV2993696.1"
                     /translation="MGIPDDLIQDIAIRELAFGAGTLHAAVASYVQSPCYYRALIAGG
                     ARYNLNGQPCGEVTPQEQKEAETRLMMLNDRRKDRKPR"
     gene            complement(917..1465)
                     /locus_tag="RH033_004632"
     CDS             complement(917..1465)
                     /locus_tag="RH033_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993697.1"
                     /translation="MQITEALLSAPGEIRRFVQQAVDHWPNLLAFHFTLHSAEGNING
                     QQIHAFCTSFYRQVHERITERNHTASPAPPVVLRWLREQHGGATMRCLLLLSQTSICH
                     PRVSATVDEECSQRVDLLQQTWRVISADGQCRVERCFQAARGDTSGQYIALKTVALSL
                     GLPVVTGITHRPVQRCTLITAQ"
     gene            complement(1743..1976)
                     /locus_tag="RH033_004633"
     CDS             complement(1743..1976)
                     /locus_tag="RH033_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDV2993698.1"
                     /translation="MLTTTSHDSVLLRADDPLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWMQQRIEESRGAAA"
     gene            complement(2000..2617)
                     /locus_tag="RH033_004634"
     CDS             complement(2000..2617)
                     /locus_tag="RH033_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993699.1"
                     /translation="MNAIPYFDYSLAPFWPSYQNKIIGVLNRVLREQSCSRVRRILLR
                     LPWEHDNAFSCRKTWLGMDFIETVSALMNATPGRDLCWILTRHPEKPEYHVVLCVRQE
                     YFDGPELDRLILDAWSNVLGFASPGEAAPYQKQITRDVVLDSRSPDCEDILKELIWAF
                     SDFARDRRGVRDPEARYLAGNPWYPVAGQLRKHSSADSPAFAQPS"
     gene            complement(2875..3441)
                     /locus_tag="RH033_004635"
     CDS             complement(2875..3441)
                     /locus_tag="RH033_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HDV2993700.1"
                     /translation="MPKSYTPNWFFTALLDNHINQMMARYSCLRALRMDFFYRKDTPD
                     FLQPDHRWLELQLRMLLEQVEQFENMVGFFWVIEWTVDHGFHAHVVFWLDRQRVKKIY
                     PFAERITECWRSITHNSGSAHRCTYQPHYTYNINIPVRHNDPESIDNIRGALHYLAKE
                     EQKDGLCAYGCNEVPERPAAGRPRKPHF"
BASE COUNT         1169 a         1003 c         1013 g         1019 t
ORIGIN      
        1 tgttcgtttg tttaaagaac ctgctggtcc tgccattaat attacctgct gattgcttca
       61 catccggatt cctcgcactg cgcgcgggtt attgtttcgt tttcggccac ggggtcgcca
      121 gggctctgtt tgcaggccat gatggcctga cctgtactgt ttctcaggca ctgagatgtc
      181 cgtttacgcc atttgttccc ggatatcctt aacctctgac gtttgttccc tgcccctctg
      241 tctgataacg aatacgctca caacgcacag tactgaacaa taccgacaac aacatgtctt
      301 acatgaaatt cgctgtaaca tccaccggaa aacattttca gatatcggat gaggtactac
      361 ttccattgac agcgcgaggt aacaaaattg atggtaactc tgaccattaa ccgtaaaccg
      421 cagggtattt tcagtaaatc accggcaacg ccgctgcaga ataaaacgga cacagcatac
      481 aaaatgaagg ccggtgacca gataccacag ttgaacgata aacagaaacc tgctgataaa
      541 acaccgtggc gacatgacaa aacgccagag caaaaaccgt aagcgcatta ttcgtcttgt
      601 ggaactctgg cctgagtcat tcaaccggga aaaaaacaaa accacttaaa gtggggatac
      661 ctgacgacct gatacaggat atcgccatcc gagaactggc gtttggcgct ggtacattac
      721 atgcagcagt ggcgtcttat gtgcagtctc cgtgctatta ccgtgcgtta atagcaggtg
      781 gcgcccgtta caacctgaac ggtcagccgt gcggggaagt gacaccacag gaacagaaag
      841 aggcagagac ccgtttaatg atgctgaatg accgacgaaa agacagaaaa cctcgttgac
      901 tgcacaacag cgctgttcac tgagctgtaa tcaatgtaca gcgctgtacc gggcgatggg
      961 taataccggt cacaaccggt aaccccagag acaatgcaac tgtttttaac gcaatatact
     1021 ggccggatgt atcaccccgg gcagcctgaa aacacctttc cacccggcat tgtccgtcag
     1081 cacttatcac ccgccatgtc tgttgcaata aatccacccg ttgcgaacat tcttcatcaa
     1141 ccgtggcact gacccgtgga tggcaaatgc tcgtctggct gagcagcaac aggcatcgca
     1201 ttgttgctcc tccatgttgt tcccgcaacc agcgtaatac caccggtggt gctggactgg
     1261 ccgtgtgatt acgttcagta atacgctcgt gcacctgtcg gtaaaaggaa gtacagaatg
     1321 catgaatctg ttgtccgttg atgtttcctt ctgccgaatg gagcgtgaag tgaaatgcca
     1381 acagatttgg ccagtggtcc accgcctgct gaacaaaacg ccggatttcc cccggcgctg
     1441 aaagtaaggc ttcggttatt tgcactattt tatccccgtt gaattttgtt aagccggtgc
     1501 agaccagaca cacggaaagc gcacatcgac accggcacgg tacgatacaa acaccagtga
     1561 ctggcgggac gaagggaatg acgaatcagt caggaaatga tgacaaacag ttggctggcc
     1621 agagggtgcc agcagaaaga tgagcgggag ttgtcagcgc cagcaccagg gacagggctg
     1681 atttccacaa taccagcgcc ggcatttcgc acattgccag cgtactggca ttacaacacg
     1741 tttcatgctg ctgctcctcg tgattcctca attcgctgct gcatccactg ctccacttca
     1801 cttttgtacc agcggctgct gcgccccagt ttgatgggtt tagggaaatg accttcactg
     1861 atcagcttgt aaaaccattt atcggtcata ccggtgaaac tggtgatgta gttcatgtcg
     1921 atcaggggat cgtcggcacg cagcaatacg ctgtcgtggc ttgttgtggt caacatggtc
     1981 agaatgtcct ttgtgagatt tatgaaggtt gtgcaaatgc gggggaatct gctgaactgt
     2041 gctttctgag ctggcctgct acgggatacc agggattacc ggcgaggtaa cgggcttccg
     2101 gatcgcgcac accacgacga tcgcgggcga aatcactgaa cgcccatatc aactctttga
     2161 ggatgtcttc gcagtccggt gaacgactgt ccagcaccac atcccgtgtt atctgtttct
     2221 ggtacggtgc cgcttcaccc ggtgacgcga agcccagtac gttgctccag gcatccagaa
     2281 tcagccggtc cagttcaggg ccatcgaaat actcctgtct gacgcacagc accacgtggt
     2341 attccggctt ttcaggatga cgggttagga tccagcagag atcacgtccg ggggttgcat
     2401 tcatcagcgc actgacggtt tcgataaaat ccatgcctaa ccaggtcttt ctgcaactga
     2461 aggcgttgtc atgttcccac ggcagacgaa gcaggatccg ccgtacccgg gagcaggact
     2521 gttcacgcaa cacacggttg agaacgccga tgattttgtt ctgataagat ggccagaagg
     2581 gggccaggct atagtcgaaa taagggatag cattcataag atatgctccg ttatgcgcag
     2641 cccacaatgc gtcctgcccg tgaataagct gacgtgttat ggtttgcgga tggggttgat
     2701 ggaataagtg aagaaccggg gcagaacggg aagatcaggt caccgacaag ggacagaaca
     2761 ccggatgtgg ttcagagtga atgatctccg ttgacttcag ttcacatcat taagcaaggc
     2821 ggtaaggttt taccgacgga atgtttccag gacaggaagc tcaggattta agcttcagaa
     2881 gtgaggctta cgaggacgcc ctgcagcagg acgttcagga acttcattgc agccgtaggc
     2941 acacagcccg tctttttgct cttcttttgc cagataatgc agggcaccgc gaatattatc
     3001 gatgctttca ggatcgttgt ggcgcacagg aatgttgatg ttgtatgtat aatgcggctg
     3061 atatgtacag cggtgtgccg aaccgctgtt atgcgtaata gaccgccagc attccgtaat
     3121 ccgctccgca aagggatata tttttttaac cctctgacga tcgagccaga agaccacatg
     3181 cgcatgaaaa ccatgatcaa ccgtccattc aatcacccag aagaagccaa ccatattttc
     3241 aaactgttca acctgctcca gcagcatacg cagttgtaat tcaagccaac gatgatcagg
     3301 ttgtaagaaa tcaggcgtat ccttcctgta gaagaaatcc atccgtaagg cccgcaggca
     3361 ggaatagcgt gccatcattt gattgatgtg attgtcaagt aaagcggtaa aaaaccagtt
     3421 tggtgtgtaa gatttaggca tgttgatgac tcctgaaagt taatgatgtt ttaagctctg
     3481 tgagcgaatt aatgctttcg ctcataatca gatctaaaac ggtaataact tacagatggt
     3541 atttatcagg catgctgtaa cagaatggat acgacggatt attaacgaca cacagcgttg
     3601 tgctgaaggg tatgagttat ctgatggcat tgtctataga gatgagataa acgaggtgcg
     3661 aagaaaatac agattcaata caacacagtg aaatacagaa aaatacgaac tgtaatcccg
     3721 ctgccaggcg gttaggaaac atattacaac tataagctca tgatttgtaa atttgctctc
     3781 agtgagtctg aaaaaacgtt tataaggtca ccacaataaa ctcacacgcg cttattcaat
     3841 ctgttctcca ggatatgaac gttaacgaca cacgatagca taagacaaca tacaatggat
     3901 gaataatttt cacccagtac cttaaacaat acaaccggca acaaatataa tcagaaattc
     3961 actctgttaa ttctgttgtt tatcctgcgc tgttaaacat gacggaacca tggatacctt
     4021 atcaaagagt aaacattcag ttaatctttg atgacagaga aattgtttcc aaccagaata
     4081 gttaaaacac ttcagataaa caatcattgt taagcaaata cagttcatga tggataaatt
     4141 aaaatcaatt actgtttcac tgaaaatgag aacagatgga tatcaacaat agcgtagctg
     4201 taga
//
LOCUS       DAPHCV010000227         4171 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.230, whole genome shotgun sequence.
ACCESSION   DAPHCV010000227 DAPHCV010000000
VERSION     DAPHCV010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.230"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..696
                     /locus_tag="RH033_004636"
     CDS             <1..696
                     /locus_tag="RH033_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDV2993701.1"
                     /translation="PAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESGRI
                     TEAACMAHARRKIHDVHARVPTDITTEALQRIGELYAIESEVRGCSAEQHLAARKARA
                     APLMQSLYDWIQQQMKTLSRHSDTAKAFAYLLKQWDALNVYCSNGWVEIDNNIAENAL
                     RGVAVGRKNWMFAGSDSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPANRV
                     RDLLPWKVDLTSQ"
     gene            1501..1701
                     /locus_tag="RH033_004637"
     CDS             1501..1701
                     /locus_tag="RH033_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993702.1"
                     /translation="MGVNMAKGESGRIVLEVEPELKKALYSVLAMEQKTLKDWFVDKA
                     QEHICEKKSELIERFSKVDNEI"
     gene            1691..3316
                     /locus_tag="RH033_004638"
     CDS             1691..3316
                     /locus_tag="RH033_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDV2993703.1"
                     /translation="MKFKADQTSQKLRGGYYTPQNLADYVTKWVLSKNPKTILEPSCG
                     DGVFIQAIANNGYNSNIELFCFELFDTEASKALERCKLNNFSNATITEGDFLVWANEC
                     LKKNKQIFDGALGNPPFIRYQFLERNFQEQAQLVFEHLDLKFTKHTNAWVPFLLSSLA
                     LLKQGGRIGMVIPSEISHVMHAQSLRSYLGHVCSKIVIIDPKEIWFEDTLQGAVILLA
                     EKKQYPDEASQGVGIVSVSGFEFLQEDPNVLFNDTAGINGETVEGKWTKATLSIDELQ
                     LIKRVIAHPDVRKFKDIAKVDVGIVTGANNYFLVDNETVKLYKLERFAHPMFGRSQHC
                     PGIIYDEKQHVENQEKGLPTNFLYIDEEFEYLSKSVKNYIKLGEVEEYHKRYKCRIRK
                     PWFKVPSVYSTEIGMLKRCHDAPRLIHNRVRAYTTDTAYRVSSTVTSTENLVCSFLNP
                     ITVITAELEGRFYGGGVLELVPSEIEKLYIPIVEGLEHNIEELNLLIKDGQIERVIRQ
                     QGSLILDKLGFTQEENEKLVEIWKKLRDRRLRK"
     gene            complement(3386..>4171)
                     /locus_tag="RH033_004639"
     CDS             complement(3386..>4171)
                     /locus_tag="RH033_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine methyltransferase"
                     /protein_id="HDV2993704.1"
                     /translation="LTRPYFETNRSGDDSFYTYKDVEPNSFVVYPYKKVGERIQFIEY
                     DELKRQYPKLFEFLQVVKVHLNDKKRSIKPDPTGPNEWYRYGRSQALENCDVDQKLIV
                     GILSNGYKYSIDNHRTFVSSGGTAGYSIINVPNNVRYSIYYIQAILTSKYLEWFASIY
                     GDIFRGRFVARGTKVQTRMPIPTIDFDDPKQKEIHDTISSKQQYLNKLYSQTQKSADR
                     DKIIFERQFEQEKIQMDYLIKNLFDLGDLDSEIPTVEDLYKNL"
BASE COUNT         1287 a          792 c          896 g         1196 t
ORIGIN      
        1 ccggcggtct ggttcgcgta cagtccggac cggaaaggta tccatccaca gaatcacctg
       61 gccggttaca gcggtgtgct tcaggcagat gcttacggtg gttaccgggc gttatacgaa
      121 tccggcagaa taacggaagc cgcgtgtatg gcccatgccc ggagaaaaat ccacgatgtg
      181 catgcaagag tgccaaccga catcaccacg gaagccctgc agcgtatcgg tgaactgtat
      241 gccattgagt cggaggtccg gggatgttca gcagaacagc atctggcagc aagaaaagcc
      301 agagccgcgc cactgatgca gtcactgtat gactggatac agcaacagat gaaaacactg
      361 tcgcgtcact cagatacggc aaaagcgttc gcatacctgc tgaaacagtg ggatgcactg
      421 aacgtgtact gcagtaatgg ctgggtggaa atcgacaaca acatcgcaga gaacgcctta
      481 cggggagtgg ccgtaggccg gaaaaactgg atgttcgcgg gttccgacag cggtggtgaa
      541 catgcggcgg tgttgtactc gctgatcggc acatgccgtc tgaacaatgt ggagccagaa
      601 aagtggctgc gctacgtcat tgaacatatc caggactggc cggcaaaccg ggtacgcgat
      661 ctgttgccct ggaaagttga tctgacttct cagtaaatat caatacggtt ctggcgagcc
      721 gcttacgcta atactatcgg aatcagcagt tctccctaaa gagacactta gcattcgacg
      781 taatagctga catagaatga ctatctcagc aggagcttaa agtgtggttt taggccgaat
      841 ttcaattgat tcatcatttg aggaatgtaa ctaatcgaca tgcttcccaa acagaaaaat
      901 cgcttctatt cccaacagaa attttgcgaa catgagcgca attatttctc tgctagggat
      961 aattggtttt tagtaaatcg ctacagcagg caattgccaa tataaaaatc tggcacaagg
     1021 gtgaacagcg cgcgccacat aatccattgt tattgctata tattagcgga atacataaat
     1081 gaacagccac gtcttttcga ttatggtcgg aaatctacaa acctctgcac agtttactag
     1141 aacttttttt ggccacagag ttcacaggac cggcctgata tgctggcgct tgaaggaaga
     1201 tagagaaaga ttaaagatgg tggatattga gttatgcaaa actgcaactt ggataatgca
     1261 gtgctactgc ggcccgattt tggcagtatc tttagtatat tcagagcggt atagccttac
     1321 acttttattc cgttcggctt tactgctaag ctctctggcc ctgcagagcc ttccccttac
     1381 catattggct agggattctt ctgacttttc aaacggttga aaagaatgct gaaaggctgc
     1441 cagttttttc tttagttgct ctgaaaaccc atgtattata tgtaacataa catataatac
     1501 atgggtgtta atatggcaaa aggtgaatca ggacgcatcg tcctagaagt tgagccagaa
     1561 ctaaaaaaag ctctctattc agttcttgca atggaacaaa aaactcttaa agactggttt
     1621 gttgataagg ctcaagaaca tatatgcgag aaaaaatcag agctcataga gagattttcg
     1681 aaggtagaca atgaaattta aagcagatca aacctcacaa aaacttagag gtggctatta
     1741 caccccacaa aatttagctg attatgtaac taaatgggtc ttaagtaaga atcctaaaac
     1801 catacttgag ccgagttgtg gtgatggtgt gtttattcag gctattgcta ataatggtta
     1861 taactctaac attgagttat tttgctttga attgttcgac actgaagcat ctaaggctct
     1921 cgaacgctgt aaactgaaca atttttctaa tgcaacgatt actgaggggg attttctagt
     1981 ctgggctaat gaatgtctta agaaaaataa gcagatattt gatggagctt taggtaatcc
     2041 tccattcatc cgttatcagt tccttgaaag aaatttccaa gaacaagctc agttggtctt
     2101 cgaacattta gatctaaaat ttacaaagca cactaatgct tgggttccat tcttactttc
     2161 ttcattagcc cttctcaaac aaggtggaag aatagggatg gtcataccat cggagattag
     2221 tcatgtaatg cacgctcaat cattgagaag ctacttaggt catgtttgct ctaagattgt
     2281 catcattgac cctaaagaga tttggtttga agatacatta cagggggccg ttattctcct
     2341 tgcagaaaaa aagcaatatc cagatgaagc atcgcaaggt gtaggtattg ttagtgttag
     2401 tggttttgaa ttccttcaag aagatccaaa cgttctgttt aatgatacgg caggaataaa
     2461 tggtgaaaca gttgaaggta aatggactaa agcaacactc agtattgacg agcttcagct
     2521 aattaaacgg gtaattgctc atccagatgt tcgtaaattt aaagatatag ctaaagttga
     2581 tgtaggtatt gtgacggggg ccaacaatta ttttcttgtt gataatgaaa cagttaaatt
     2641 atataaatta gaacgttttg ctcaccctat gtttggcaga agccaacatt gccccggcat
     2701 tatttatgat gagaagcagc acgttgaaaa ccaggaaaag ggtttaccaa ccaatttttt
     2761 atatatagat gaagagtttg aatatctttc taaaagtgtc aaaaattaca taaagttagg
     2821 cgaagtagag gaataccaca agcgttataa gtgccgtata cgtaagcctt ggttcaaggt
     2881 cccatctgta tatagcacag agattggaat gttaaagcgt tgtcacgatg cgccacgctt
     2941 aattcataac agagttagag catataccac tgatactgca tatagagttt cttcaacagt
     3001 tactagtact gagaacttag tgtgtagttt tttgaaccct ataacggtca ttacagccga
     3061 actcgaaggg cgcttttacg gcggtggcgt tcttgaactt gtcccatccg agatagaaaa
     3121 actatatatc cctatcgttg aaggattaga acataacatt gaagaactca acctattgat
     3181 taaagatggt caaatagaga gggtaattcg acaacaaggt tcactaatct tagacaagct
     3241 tggtttcact caagaagaaa atgaaaaact tgtagaaata tggaagaagc ttagagatag
     3301 aaggctacgt aagtagggtt taaaaagtag ggggctcata aaaaatcaat gcagctttta
     3361 gctgcattga ttttatctgg taaccttata ggtttttgta tagatcttct actgttggaa
     3421 tctcggaatc taaatccccc aagtcaaaaa gattcttaat caaataatcc atttggattt
     3481 tttcttgttc aaattgcctt tcgaatatga ttttatctct atcagctgat ttttgagttt
     3541 ggctatacag tttattcaaa tactgttgct ttgaactgat cgtatcatgt atttctttct
     3601 gttttggatc atcaaaatca atagtaggaa taggcattct agtttgaact tttgtcccac
     3661 gagcaacaaa tctacctcta aaaatatcac catatattga agcaaaccac tcaaggtact
     3721 ttgatgtaag gattgcttgg atataataga tagagtatct aacgttatta ggaacgttga
     3781 taatgctata gcccgcagtg cctccagaag aaacaaaagt tctatggtta tcaatgctgt
     3841 acttatatcc attagataga ataccaacaa tcagcttttg atcaacatcg cagttttcta
     3901 atgcttggct acgtccgtat ctgtaccatt cattaggccc agttgggtca ggcttaatag
     3961 agcgtttctt atcattcaaa tggactttta caacctgcag gaactcaaat aacttaggat
     4021 attgacgttt tagttcatcg tattcaatga actggattct ttcacccact ttcttgtatg
     4081 ggtaaacaac aaatgaatta ggttcaacgt ctttataagt atagaaacta tcatcacctg
     4141 aacgattcgt ttcgaagtaa gggcgagtta a
//
LOCUS       DAPHCV010000228         4163 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.231, whole genome shotgun sequence.
ACCESSION   DAPHCV010000228 DAPHCV010000000
VERSION     DAPHCV010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.231"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(581..814)
                     /locus_tag="RH033_004640"
     CDS             complement(581..814)
                     /locus_tag="RH033_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDV2993705.1"
                     /translation="MLTTTSHDSVLLRADDPLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWMQQRIEESRGAAA"
     gene            complement(883..1455)
                     /locus_tag="RH033_004641"
     CDS             complement(883..1455)
                     /locus_tag="RH033_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993706.1"
                     /translation="MNAIPYFDYSMAPFWPSYQNKIIGVLNRVLREQSCSRIRRILLR
                     LPGEHGNIFSDRKTWFGMAFIETVSALMNATPGRDLCWLLTRHPEKPEYHVVLCVRQE
                     YFDGPELDRLILDAWSNVLGFASPGEAAPYQKQITRDVVLDSCSPDCEERLKELIWAF
                     SDFARDRRGVRDPEARYLAGNPWYPVAGQL"
     gene            complement(1812..2543)
                     /locus_tag="RH033_004642"
     CDS             complement(1812..2543)
                     /locus_tag="RH033_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HDV2993707.1"
                     /translation="MNQPIHNAYWLSRFESILNSALAQHRAVSLIRVDLRFPEYMPAT
                     IMDTDLDSAVISRFFASLKAKIQAYQRHKRRANKRVRATTLRYFWCREFGKEKGRKHY
                     HVILLLNKDTWCSPGDFTVPSSLATLIKLAWCSALHLEPWQGNGLVHFSRRTPFRKPT
                     SSDARPSSDDTPLSGGCSDTRKASDKKPGEAAVLWIKRGDVEALQKARNRASYLVKYE
                     TKQHDGSGQRNYGCSRGPGRLLDGR"
BASE COUNT         1254 a          974 c          885 g         1050 t
ORIGIN      
        1 aaagtatctt atcttctggt agagatacag gatacagttt gtaggagctt tctttttttg
       61 aacaatgatt tgcaggacag ttatgccaac ctagcagaat cagtatcaca acaataaatg
      121 acagatcata cctgaaattt gaattaacag gcatcatgaa tgtcaatagt ctaagcggtt
      181 actacttaca tcgcttgcat aaatactaaa cactgactag aaaagtattg gataaccccg
      241 tcactaaaag taacacttcg gttatttgct cggaatgtat caacgccaag attgttagcc
      301 gttacgcctt atatcactgg cgctgacaaa tgtttctttt ctaatttact gaaccggcgc
      361 agacttacag aaaaacactc cggcaccggt gtaatgccag ttataatcaa taagaataaa
      421 atgacgaatc agtcaggaat agctataacc tgcaggctga ctggagaatg tctgcaagaa
      481 gatgaacgta aatgtcgcca gcaccagggg cagggccggg ttccacaata ccagcgctgg
      541 cattttgcac attgccagcg taccggcatt acaacacgtt tcatgctgct gctcctcgtg
      601 actcctcgat tcgctgttgc atccactgct ccacttcact tttgtaccag cggctgctgc
      661 gccccagttt gatgggttta gggaaatgac cttcactgat cagcttgtaa aaccatttat
      721 cggtcatacc ggtgaagctg gtgatgtagt tcatgtcgat caggggatcg tcggcacgca
      781 gcaatacgct gtcgtggctt gttgtggtca acatggtcag aatgtccttt gtgagattta
      841 tgaatgttgg gcaaatgcgg gtgaatctgc tgagctgagc tttcagagct ggcctgctac
      901 gggataccag ggattaccgg cgaggtaacg ggcttccgga tcgcgcacac cacgacgatc
      961 gcgggcgaaa tcactgaacg cccatatcag ctctttgagt ctctcttcgc agtccggtga
     1021 acaactgtcc agcaccacat cccgggttat ctgtttctgg tacggtgctg cttcacccgg
     1081 tgacgcgaag cccagtacgt tgctccaggc atccagaatc aaccggtcca gctcagggcc
     1141 gtcgaaatat tcctgtctga cgcacagcac cacgtggtat tccggttttt caggatgacg
     1201 tgtcaggagc cagcagaggt cgcgtccggg ggttgcattc atcagcgcac tgacggtttc
     1261 gataaaggcc ataccgaacc aggtttttct gtcgctgaag atgttgccat gttcccccgg
     1321 cagacgaagc aggatccgcc gtattcggga gcaggactgc tcgcgcaaca cacggttgag
     1381 aacgccgatg attttgttct gataagatgg ccagaagggc gccatgctat agtcgaaata
     1441 agggatagca ttcataagat atgttccgtt atacgcagcc cacaacgcgt cctgcccgtg
     1501 gatgcgctga cgtgttatgg cttgcgtatg gggttgatgg aataagtgaa aaaccgcggc
     1561 agaacgggaa gatcaggtaa ccgacaaggg acagaacacc ggatgcaggt tcagaatgaa
     1621 tgctctccgt tgacttcggt tcacatcatt aagcaggaca gtaaggtttt accgacggag
     1681 tgtttccagg agcgaaagca gagaaaaata ccgttgcatg acttcaggac gactttttca
     1741 gaaaacgagc taagggcgtc agccaacgtc ctgtagcata agctcagaca ccggatgtcg
     1801 ttttacaggg ctcacctgcc atccagcaga cgccccggcc cacgactgca accataatta
     1861 cgttgcccgg aaccgtcatg ctgcttcgtc tcatatttca cgagataact ggctctgttc
     1921 cgggctttct gcagcgcttc cacatcacca cgcttgatcc agagaacagc ggcttcaccc
     1981 ggctttttgt ctgaagcctt cctggtatca gaacatccac ccgacaaagg cgtatcatcg
     2041 gaagaagggc gagcatcaga tgatgtcggt ttacggaaag gcgtccgcct ggaaaaatga
     2101 accagtccat taccctgcca gggctcaaga tgcagagcac tacaccatgc cagcttgatc
     2161 agtgtcgcca gcgaagaagg aacggtgaaa tcccctggcg agcaccaggt atctttgttg
     2221 agcagtaata tcacgtgata atgtttcctg cctttttcct tgccaaactc ccgacaccag
     2281 aaataacgca gagtggttgc acgcacacgc ttgttggcac gtcgtttatg tcgctggtaa
     2341 gcctgaattt ttgctttcag ggatgcaaaa aaacgagaaa tcactgctga atctaaatcg
     2401 gtatccataa tagtggcagg catatactca ggaaaacgta aatccacccg gattaacgag
     2461 acggcacggt gttgcgccag ggcactgttg agaatacttt caaaacggga taaccagtaa
     2521 gcattgtgta ttggttgatt catgatgaga gatctccgtt gatttggatt gacttcacat
     2581 cattaatcac tgcggtaaaa ctttacggca tcccgccatt gagcaacgcc aatggctcct
     2641 gattaaaaca gaatgaatcc cccacgaaga aaagataaac ctacacgcag ggtaaaacac
     2701 acaacaacgg cataaatggt ttttaaaaac tacaaagccc gaagggaaac ggatccacag
     2761 aacgtgcaaa caaaacacca aaaacgcaat cagaacgctt tctgttgctg tgaatcaaca
     2821 acaacgccaa aacctacagc ttaatcacac aactcaatca cggatgcgaa tgacacgttt
     2881 aaaacctgtt tacagataac gccgttacac gatcatcccc atcagaatcg attttccctt
     2941 cctgaaagcg ttacgtttca ctacgcacca taaaaacgat taaccatcga taacgtaaac
     3001 ttcatctgaa tcaaaacacc aaaaccgttc atcacacttt cgcaatgcaa gaagagtcac
     3061 atcatgacga aaacaatgac cttacacgga aaagaaaaac gtcaggaatc gttaatgcct
     3121 aaatctgatt gttatcaaaa catccagaaa gtcgtttaac agaatcgtcg taagcacatc
     3181 gaaaaaaacg tcagattact tcaggaaagt tcgggttgat ttagcgtaaa gatacttttc
     3241 tcatgctaac gtcgaaactc gaaatctcag aaaaggttaa acgcaacatc agtaaaatcg
     3301 aatagcagat tgattgctta aacagaaagg ttttacagca gctttaaact gattttagct
     3361 aacgtaaaga tttactaaac tggatgtgtt tcagacaaag caatgtgtac agcaatgctt
     3421 aatgaaaaca cttatagggc tgtttatgaa aacacgtgta tgtctggtaa aacacttagt
     3481 agaaaatacg tgtataacac ttactgaaat gcttagtaaa aatacttagt aaaatacgtg
     3541 tatatagtat cataccgaca ttagcaacat tctcagacac tcatgcagac tgaatgttga
     3601 ttacccgttt tcacatcagg catggttctt tactgaccaa aaaacaaact ggtttactct
     3661 tgaattattt tctctacctc agaaaacatg gtctcaggaa tctgctatgg tttcatagtt
     3721 tctgttgttc ttttcataaa agttataagt cattgtcata atcatacgat aatcacttta
     3781 tgttagtttt tactgtcaca cgctactgac tttttcagtc attgccagaa aggggctctt
     3841 tttacttcca tttcaccact caacaaatgc aaaacttgtc cagtaaagcg gaccacctct
     3901 tgctcacaag caacgaatca tctccaggaa cagccctcaa acattgcaga aacaaaaaca
     3961 acaaaaccct cattatcact gatgttcgga aaatttcccg aaacaaaaaa aacgaaatca
     4021 aaatgagact cattatcatg gatgtagtca aaaagacgtt atggtggctt tcagttaaat
     4081 ggtactattt ctcataaatc cacttaactt tttttactga ttcatcccag ataaccactt
     4141 tatcagtaaa aaatgaatta tat
//
LOCUS       DAPHCV010000229         4138 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.232, whole genome shotgun sequence.
ACCESSION   DAPHCV010000229 DAPHCV010000000
VERSION     DAPHCV010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.232"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..197
                     /locus_tag="RH033_004643"
                     /pseudo
     CDS             1..197
                     /locus_tag="RH033_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            870..2378
                     /gene="ltrA"
                     /locus_tag="RH033_004644"
     CDS             870..2378
                     /gene="ltrA"
                     /locus_tag="RH033_004644"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDV2993708.1"
                     /translation="MQRKLATWAVTDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFSKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
     gene            2468..2941
                     /locus_tag="RH033_004645"
                     /pseudo
     CDS             2468..2941
                     /locus_tag="RH033_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2982..3703)
                     /locus_tag="RH033_004646"
                     /pseudo
     CDS             complement(2982..3703)
                     /locus_tag="RH033_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3698..4019)
                     /locus_tag="RH033_004647"
                     /pseudo
     CDS             complement(3698..4019)
                     /locus_tag="RH033_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1025 a         1010 c         1139 g          964 t
ORIGIN      
        1 agttgttacg ggaaggaatc agggtagcca gatgtacagt gtcgcgcctc atggcggtta
       61 tgggacttgc cgatgttctc cggggtaaaa aggtccgcac taccgtcagc cggaaagccg
      121 ttgccgcagg cgaccgcgta aaccgtcagt ttgtggcaga acgtcctgac cagttgtggg
      181 tggctgattt tacttacggt gtgcccggag ttcagggcgg gcatggatgc ttaaatgaac
      241 cgcgagtctg tctggaatat tgaaccggta actcacgatg agaaacccaa caatcccacc
      301 gggtgtgacg gtggagaacc tgagcggcag tgacctgcgg catgcccgca gggtgatgta
      361 acccgctgac aacggggatt gaggcgagat cactaagccg agatgatcct caaggttaag
      421 tactgaaagg ctgaagaaca tgaacccgtt aatccgcctc tgtgggttga aaacgtcacc
      481 acggcctacg tgatctgaca ggccgtgcag gaggaactgg cagtgatacg taagcactgc
      541 cggtcgaagg tgttttgaca tgtatgcgaa acactggggc agcagcgtct atcacgcttg
      601 cgttgctgac ttctgccaac ttgcggcaag caaggataaa gagtgcgacg ggcagcctcc
      661 tcagtatgcc tgagtccagg caggtaaacc ggggaaggtc agcgacggat gttaaggggg
      721 catggctccg atgacgcgct ggctggcgga gcttccgtag tagtccgcga tggggaaagc
      781 ccattacatg gcgaagggaa gcagtttgaa tgtgtttgcg acgtgaatta actgacctaa
      841 cgaggtgaag acctttgata atcagcgaaa tgcaacgcaa gcttgccaca tgggcagtca
      901 ccgatccgtc cctacggatt caacggctgc tgcgtctgat aacacaacca gaatggctgg
      961 ctgaagcggc gcggatcacg ctttcatcaa agggggccca tacccccggc gttgatggcg
     1021 tgaacaaaac aatgctacag gccagactgg ctgttgagct gcaaatcctc agggatgaat
     1081 tactctcagg ccactaccag cccttgcccg ccagacgggt ttacatccct aaaagcaacg
     1141 gcaaactgcg accactgggt atccccgcgt tgcgggatcg tattgttcag cgggccatgc
     1201 tgatggcgat ggagccgata tgggagagtg attttcatac gctctcatat ggcttccggc
     1261 ctgagcgcag tgtccaccac gctatccgca cggtgaaatt acagctcaca gactgtggtg
     1321 aaacccgggg acgctgggtg attgaaggcg acctgtccag ttacttcgac accgtacatc
     1381 atcgactgct gatgaaggct gtacgccgca ggatcagtga cgcacgtttc atgactctgc
     1441 tgtggaaaac catcaaggcg ggacatatcg atgtcggtct ctttcgggcg gccagtgaag
     1501 gtgtgccaca gggcggtgtt atatcgccgc tattatcgaa catcatgctg aatgagttcg
     1561 atcaatacct gcatgagcgc tacctgagcg ggaaagccag aaaagatcgg tggtactgga
     1621 ataacagtat ccaacggggc cgaagtacgg cggtcagaga aaactggcag tggaaacccg
     1681 cggtggcgta ctgccgctat gccgatgatt ttgtcctcat cgtcaaaggc accaaagcac
     1741 aggcggaagc catcagggag gagtgtcggg gtgtgctcga aggcagtctg aaactcaggc
     1801 tgaacatgga taagactaaa atcacccatg ttaatgacgg ctttatcttt ctggggcaca
     1861 ggatcattcg caaacgcagt cgttatggcg agatgcgagt ggtctcaacg atcccgcagg
     1921 agaaagccag aaacttcgcc gcatcgctga cagcactgtt atcaggcaac tacagtgaaa
     1981 gcaaagtcga tatggctgaa caactcaacc gaaaactgaa aggctgggcc atgttctatc
     2041 agttcgttga ttttaaggcc aaagtcttca gttatatcga ccgtgtcgtg ttctggaagc
     2101 tggctcactg gctggcccgc aaataccgta caggtatcgc ttccctgatg aggtggtggt
     2161 gtaaatcacc gaaaccgggt cagagcaaaa cgtgggtttt atttagtaaa accaatcacg
     2221 gcaagctcag cggcgaaata ctgtaccggt tggtggggca aggcaagaag ctgttccgct
     2281 ggcggctacc cgaaggtaat ccctatctga ggacggagac cagaaacacg tatacatcgc
     2341 gctttacaga agtggcaatg gcgttcgcca gcatttaaat ggagagccgg atgcgctgaa
     2401 aggtgcacgt ccggttcggg gaggagaggc agggaaatag tccgactacg ccctgcctct
     2461 tactctactc agcacatggc tggggggcgt ctatgtggcg ttcatcattg atgtgtttgc
     2521 cggatacatc gtggggtggc gggtctcatc gtccatggaa acgacattcg tgctggatgc
     2581 actggagcag gcgttatggg cccgtcgacc gtccggcacg gtccatcaca gtgataaagg
     2641 ttctcagtat gtatcgctgg cctacacaca gcggcttaag gaagccggat tactggcatc
     2701 aacaggaagt acaggcgact cgtatgacaa cgcgatggcg gagaacatca atggtcttta
     2761 caaagcggag gtaatacacc gtaagagctg gaaaaaccgt gcagaagtgg aactggccac
     2821 actcacgtgg gtggactggt ataacaatcg acgattgctg gaaaggctgg gccatactcc
     2881 tccggcagaa gcagaaaaag cttattatgc ttccatcgga aacgatgatc tggcagcctg
     2941 agttcacaga taaaacactc tccaggaaac ccggggcggt tcagaacgac ctccccctta
     3001 tttccggtga aaagaaggtt ttttcccgca ttcaggtcat ttcctttttt gccgtgcata
     3061 tcccagcttg tatttccctg ggtcagggtg ccggtaccat tagcagcatc atatttccac
     3121 tgcagtgctt ccgtaccaga tgtgtaggtt atggttttgt caaaatcatt ttgcggctgt
     3181 tgaccgagga agtcctgagt ggtgacgacc cagttattgc ctgaagaggt cattgaagat
     3241 gtaacaccaa tcaacaccat ttttttcccc aggaatcata agcaaatagt ggagagccac
     3301 tatcccccat aattagatag tttgctaaag gcccctgcga tgggttaaaa atattagcgc
     3361 ttggaatggt aatcatctgc ccgttattat aggagtttag tgcaccaact gtaccaccga
     3421 ccaaaaaaac accatttgta taaatctgtg tgaggttacc gtttttgtcc ttgataaact
     3481 gctgtccgcc accaagacga tagaatgccg taaaacggct ttctgcctga taggctccgc
     3541 tcaccgcccc cacatcagac acttcagtcg gagcaacctc tgtcaccagt ttactcaggc
     3601 gacggttttt tatatcaaga ccactattat tgtttgtgcc aactgcagta taagtgtttc
     3661 tggcgtaccc gaagctcatc gtatcggagc cgttaacatg tttttcaata aaaacatact
     3721 tcattacagg cgcttcgcga agtatgtcgc tgccttttgg aggatggcca gctcctctac
     3781 ctgctccgcc agttgccgct tcaggcgggc attttcagcg gccagctcac tttcacgcta
     3841 tggcgaggct atttattgct gcagtttgct gcgccacgca tagagctgag attcatacag
     3901 gctgagttca cggactgcgg cagcaatacc aatgcgttca gcaagcttca gggcttcact
     3961 gcgaaattca ggcgaatgct gtttacgtgt tttttgctgg tttatactgt ttttgtcatg
     4021 tgagtcacct ttgactgaaa gtttactcac ttagccgcgt gtccactatt gcttggtaag
     4081 atcacgtcta gatttttatc aatgagttgc tgtacgattg actgataaga ttaccgca
//
LOCUS       DAPHCV010000230         4097 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.234, whole genome shotgun sequence.
ACCESSION   DAPHCV010000230 DAPHCV010000000
VERSION     DAPHCV010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.234"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            130..345
                     /gene="bdm"
                     /locus_tag="RH033_004648"
     CDS             130..345
                     /gene="bdm"
                     /locus_tag="RH033_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HDV2993709.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            447..584
                     /gene="sra"
                     /locus_tag="RH033_004649"
     CDS             447..584
                     /gene="sra"
                     /locus_tag="RH033_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HDV2993710.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            741..2438
                     /gene="maeA"
                     /locus_tag="RH033_004650"
     CDS             741..2438
                     /gene="maeA"
                     /locus_tag="RH033_004650"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDV2993711.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWHAEYRDYRRTSI"
     gene            2572..3582
                     /gene="adhP"
                     /locus_tag="RH033_004651"
     CDS             2572..3582
                     /gene="adhP"
                     /locus_tag="RH033_004651"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HDV2993712.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPCVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            3728..4012
                     /locus_tag="RH033_004652"
     CDS             3728..4012
                     /locus_tag="RH033_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDV2993713.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
BASE COUNT         1075 a          998 c         1078 g          946 t
ORIGIN      
        1 taaaatataa aactcatcaa ccaggactaa tcttaacaac gaagcggcaa atatttgcca
       61 tgctgaatgt gctttataaa tctgcgatcc gtagcagaca ccataaatac acagacacgg
      121 agaatcacta tgtttactta ttatcaggca gaaaattcaa cagcagaacc cgctctggtt
      181 aatgccattg agcaaggcct tcgggcagag cacggtgttg tcactgaaga tgacattctc
      241 atggagctga ccaaatgggt ggaagcttct gataacgaca tcctcagtga tatctaccag
      301 caaacgatca attatgtggt cagtggccag caccctacgc tttaaggtgc tatgcttgat
      361 cggcaaccta atttaggggt ttagcacgtg tttcttcgct acggcgatgt tgtccttaaa
      421 actagcaaca ggattgagga gttaaaatga aatcgaaccg tcaggcacgt catattcttg
      481 gactggacca taaaatttct aaccagcgca aaatagttac cgaaggtgac aaatccagcg
      541 tggtaaataa cccaaccggc agaaaacgcc ccgctgaaaa gtaattcata accatcagtc
      601 ctcaatgacg attaaacacc attgcctgcg caatggtgtt tttgttttta tctgctttat
      661 acttggggcc gacgccctgg cggtaaagca aagacgataa aagcacccca gggatggata
      721 ttcaaaaaag agtgagtgac atggaaccaa aaacaaaaaa acagcgttcg ctttatattc
      781 cttacgctgg ccctgtactg ctggaatttc cgttgttgaa taaaggcagc gccttcagca
      841 tggaagaacg ccgtaacttc aacctgctgg ggttactgcc ggaagtggtc gaaaccatcg
      901 aagaacaagc ggaacgagca tggatccagt atcagggatt caaaaccgaa atcgacaaac
      961 acatctacct gcgtaacatc caggacacta acgaaaccct cttctaccgt ctggtaaaca
     1021 atcatcttga tgagatgatg cctgttattt ataccccaac cgtcggcgca gcctgtgagc
     1081 gtttttctga gatctaccgc cgttcacgcg gcgtgtttat ctcttaccag aaccgccaca
     1141 atatggacga tattctgcaa aacgtgccga accataatat taaagtgatt gtggtgactg
     1201 acggtgaacg tattctgggg cttggtgacc agggcatcgg cgggatgggc attccgatcg
     1261 gtaaactgtc actctatacc gcctgtggcg gcatcagccc ggcgtatacc cttccggtgg
     1321 tgctggatgt cgggacgaac aaccaacagc tgcttaatga tccgctgtat atgggctggc
     1381 gtaatccgcg tatcactgac gacgaatact atgaattcgt tgatgaattt atccaggctg
     1441 tgaaacaacg ctggccggac gtactgttgc agtttgaaga cttcgcacaa aaaaatgcga
     1501 tgccgttact taaccgctat cgcaatgaaa tttgttcttt taacgatgac attcagggca
     1561 ctgcggcggt aacagtcggc acactgatcg cagccagccg cgcagcaggt ggtcaactga
     1621 gcgagaaaaa gattgtcttc ctcggcgcag gctctgcggg gtgcggcatt gccgaaatga
     1681 tcatcgccca gactcagcgt gaaggattaa gcgaggaagc ggcgcggcag aaagtcttta
     1741 tggttgatcg ctttggcctg ctgaccgaca agatgccgaa cctgctgcct ttccagacca
     1801 aactggtgca gaagcgcgaa aacctcagtg actgggatac cgacagcgat gtgctgtcac
     1861 tgctggatgt ggtgcgtaat gtaaaaccag atattctgat tggcgtctca ggacagaccg
     1921 ggctgtttac ggaagagatt atccgtgaga tgcataaaca ctgtccgcgt ccgatcgtga
     1981 tgccgctgtc taacccgact tcacgcgtgg aagccacacc gcaggacatt atcgcctgga
     2041 ctgaaggtaa cgcgctggtc gccactggca gtccgtttaa cccagtggta tggaaagata
     2101 aaatctaccc tatcgcccag tgtaacaacg cctttatttt cccgggcatc gggctgggcg
     2161 ttattgcttc cggcgcgtca cgtatcaccg atgagatgct gatgtcggca agtgaaacgc
     2221 ttgctcagta ttcgccgctg gtcctgaacg gcgaaggtct ggtactaccg gaactgaaag
     2281 atattcagaa agtctcccgc gcaattgcct ttgcggttgg caaaatggcg cagcagcaag
     2341 gcgtggcggt gaaaacgtct gccgaagctc tgcaacaggc cattgatgat aatttctggc
     2401 acgccgaata ccgcgactac cgccgtacct ccatctaagc ctgcgcccgg tggtgaaagc
     2461 tgccgggcaa ttttcctctc cctttttcag atctcatcca tactgggtag tggcgaataa
     2521 atctcatttg cctcacctgc tatgcagaac atcatccgaa aaggaggaac tatgaaggct
     2581 gcagttgtta cgaaggatca tcatgttgac gttacggata aaacactgcg ctcactgaaa
     2641 catggcgaag ccctgctgaa aatggagtgt tgtggtgtat gtcataccga tcttcatgtt
     2701 aagaatggcg attttggtga caaaaccggc gtaattctgg gccatgaagg catcggtgtg
     2761 gtggcagaag ttggcccatg tgtcacctca ttaaaaccag gcgatcgtgc cagcgtggcg
     2821 tggttttacg aaggatgcgg tcattgcgaa tactgtaaca gtggtaacga aacgctctgc
     2881 cgttcagtta aaaatgccgg atacagcgtt gatggcggga tggcggaaga gtgcatcgtg
     2941 gtcgccgatt acgcggtaaa agtgccagat ggcctggact cggcggcggc cagcagcatt
     3001 acctgtgcgg gggtcaccac ctataaagcg gtgaaactgt caaaaattcg tcccggtcag
     3061 tggattgcta tctacggtct tggcggtctg ggtaacctcg ccctgcaata cgcgaagaat
     3121 gtctttaacg ccaaagtgat cgccattgat gtcaatgatg agcagttaaa actggcaacc
     3181 gaaatgggcg ctgatttagc gattaactca cgcactgaag acgccgccaa aattgtgcaa
     3241 gaaaaaaccg gtggcgctca cgccgcggtg gtgacagcgg tagctaaagc tgcgtttaac
     3301 tcggcggttg atgctgtacg agcaggcggt cgtgttgtgg ctgtcggtct gccgccggag
     3361 tctatgagcc tggacatccc acgccttgtg ctggatggta ttgaagtggt cggttcgctg
     3421 gtcggcacgc gccaggatct aaccgaagcc ttccagtttg ccgccgaagg caaagtggtg
     3481 ccgaaagtcg ccctgcgtcc gttagcggac atcaacacca tctttaccga gatggaagaa
     3541 ggcaaaatcc gtggccgtat ggtgattgat ttccgccgct aagaggcctt tgctgcgact
     3601 gccatgtttg ggtcgcagca tcacacactc tccaacatga aatggctaaa tggattattg
     3661 ttgcgtagga aggaacaata atgatagtgt tcctggtgaa ggaacgctac tgagagtaaa
     3721 gacactaatg aaaatggcca atcatccccg cccgggggac attattcagg aatcactgga
     3781 cgaacttaat gtcagcctgc gcgagtttgc cagagcaatg gaaattgcgc cctcaacggc
     3841 aagccgattg ctgaccggaa aagcagcttt gacgccagaa atggcgatta aactttccgt
     3901 ggtgatcggc agttcgccgc aaatgtggct gaatctgcaa aacgcctgga gcctggcaga
     3961 ggctgaaaaa acggtggatg tgtcgcgact gcgccgtctg gttacgcaat aataaatatc
     4021 ctccggcata gccggaggtt tttcaaatgc gcctataagg ctctcttacc agccgcgccc
     4081 taacaggcgc acacgat
//
LOCUS       DAPHCV010000231         4073 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.235, whole genome shotgun sequence.
ACCESSION   DAPHCV010000231 DAPHCV010000000
VERSION     DAPHCV010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.235"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..591
                     /locus_tag="RH033_004653"
     CDS             <1..591
                     /locus_tag="RH033_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993714.1"
                     /translation="KKNPSMANTKIIAPHDDIIAHTDFYTWQAVLSDAFHTQSRSEAS
                     RALWPRLTYRVLKGLDRSKDEGTARIDFLNELNEIRNYRNRLSHNDCIWIKIHSKNLQ
                     SAVETIREKINKIEGLIKTINPQVHLSLTKWGSFYHAKRICSQKEAELHLGKGIINST
                     TDEMNTILDQLYALTADGKLTGVVKRNTNNIAFHKF"
     gene            1185..1778
                     /locus_tag="RH033_004654"
     CDS             1185..1778
                     /locus_tag="RH033_004654"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDV2993715.1"
                     /translation="MSGRKFGRLPNWWARSSWLVNNVRSNEIGGGIASMKCLLALSVL
                     IDFHSRTASVSFTGMERLTGLSRPMIVKGVAKLEKDGLIKIDREMFVNSYELTVQPND
                     DRWAKVPVDTIRKKLNTISNRGMAPFVALKLYLTIISLRYQSNNTVKVTHETLRSYTG
                     VQPNQIRFGLDVLYCSRLIHLQPKEDRESNIYEIIGL"
     gene            2551..2745
                     /locus_tag="RH033_004655"
     CDS             2551..2745
                     /locus_tag="RH033_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HDV2993716.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHEL
                     AEELQNSIQVRFETESETGT"
BASE COUNT         1331 a          929 c          869 g          944 t
ORIGIN      
        1 aaaaaaaacc catcaatggc aaataccaaa ataattgcac ctcatgatga catcattgca
       61 cacacagatt tttacacatg gcaagcggtt ttgagcgatg cttttcatac acaatcaaga
      121 tctgaagcat caagagcctt atggcccagg ctcacctaca gagtactgaa gggattagat
      181 agaagcaaag atgaaggcac agccagaatt gatttcttaa acgagctgaa tgagatcaga
      241 aactacagaa ataggttatc acataatgat tgcatatgga taaaaataca ctctaaaaat
      301 ttgcaatctg cagtagaaac aataagagaa aaaataaaca aaatagaagg actgataaaa
      361 acgataaacc cacaggtaca cctatcatta acgaagtggg gttcctttta tcacgccaaa
      421 agaatatgtt ctcaaaaaga ggccgaattg catctaggca aaggaataat caatagcaca
      481 acagatgaga tgaacacaat tctagatcaa ctatacgcac taactgctga cggaaagcta
      541 acgggtgttg taaaacgaaa taccaacaat atcgcatttc acaagtttta gctcacaaaa
      601 tagaaaaggc ccgacagttt cctgtcgagc ctcatccaaa tctcttgcca ttatcacctt
      661 tcggtggagc gtggccttaa tgtcattgtg aatctataaa aatttcacaa tgcattaaag
      721 cgaaagctca aggcttcact gccatacatg atacatcatc atcaggaaaa agagcaaact
      781 atttcccatg atcacgccaa agcttaccat ccaccattta ccaatagatc aaaaaacaca
      841 caacaaaaat aactaaaaac gccgattaaa ccagcattat caaatttaca tttaccagtt
      901 aaaaaacgat aaaaacaaga taacatcatg ttttatatgg aaaaacaagc agccaccaaa
      961 tattgcttta tctagattta ttctaataag cgtatataaa gttaaaacac aacaacttag
     1021 tatgcgactg gaggaccaat tggaaaccct caacaatttg ctggtaacgc taacttccgc
     1081 aaagccggag gttttggttt ctgttgtatc tttaggtgca atggttttca tgtatatgat
     1141 aataaagcta tttatatctc tccttgaccg aaaatggggt aaaaatgagc ggtagaaaat
     1201 ttggcaggct tccaaactgg tgggcccgaa gctcatggct tgtaaacaat gttcgttcaa
     1261 atgagatagg gggcggcatt gcatcaatga aatgcttgtt agctctatcc gtcttgattg
     1321 atttccattc aagaacagcc tcagtatcat ttaccggaat ggaaagactg actggactaa
     1381 gccgccccat gatagtgaaa ggagtagcca aacttgagaa agatggtctt ataaagatag
     1441 atagagagat gtttgttaac tcctatgaat taacagtaca gccaaatgat gataggtggg
     1501 caaaagtacc agtagatact atcaggaaaa aactaaacac catatcgaac agggggatgg
     1561 ccccatttgt ggcattaaaa ctatatttaa caattataag ccttcgttat cagtcaaata
     1621 atactgttaa ggtgacacat gaaacactga gaagctatac aggtgtccag ccaaaccaga
     1681 ttaggttcgg cttagatgta ctgtactgta gtcgcctgat acatctgcaa ccaaaagaag
     1741 atagagagtc aaacatctat gaaataatcg gcctatgagg aaatgcattt accataacgt
     1801 cgcattacgc gcaccgctaa aacgcactga acgcgattct gacgcagata ccgcaaaaag
     1861 agatgtttta cccagtgaaa accgaaaaaa tgcgtcagga tcgttttcag cccactgcat
     1921 agctatgcat gaaaatgaat gacgaacggt ttgggcgcat acggtgccca tgagacacat
     1981 tttcggacat tttcagttcg tgaatccctg actcattgct acatatctga acagacaccc
     2041 ctggcagcgc ccccaggggc gtcctgtaaa aaaacacacc gcgccgctag cagcacccct
     2101 gatataaaat gatgtttttt ataaaaatag tcagtaccac ccctacaaag cggtgtcggc
     2161 gcggtgttgt agctgcgccg gcaccgcttt tttaaatatc gcaaagagag caagagaaac
     2221 taatttttca taactctcta tttataaaga aaaatcagca aaaacttgtt tttgcgtggg
     2281 gtgtgggcat ttcaggaatg agaacagact cgcgtttttc cggagtatca gcggaattat
     2341 tgattaagaa acactcgacg cagagcgaaa ttttatgcaa cagcggctgc actgcacagg
     2401 gattatttat cagttgatat gctgtgccat gatacgtcct aaaaaaacag gacaggatgt
     2461 gcaattgtaa taccgtcgcc cgcaacgtct tacaattacc agctgctcag ggtccccccg
     2521 acacccggaa ctgacggaga acaacggaag gtgaacaaac aacaacaaac tgcgctgaat
     2581 atggcgcgat ttatcaaaag ccagagcctg acactgcttg aaaaactgga tgctctggat
     2641 gccgacgaac aggcagccat gtgcgaacga ctgcacgaac tcgcggaaga actccagaac
     2701 agcatccagg ttcgctttga gaccgaaagt gaaacaggaa cataacgaag ctgacggagg
     2761 cgctcacggc ttgtccgcga gcgttttcgg cagcagacaa gcccgtcagg gcgcgtcagg
     2821 gggttttagc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
     2881 tactggctta accatgcggc atcagtgcgg attgtatgaa aagtgcccca tgccgggtgt
     2941 gaaatgccgc acagatgcgt aaggagaaaa tgcacgtcca ggcgcttttc cgcttcctcg
     3001 ctcactgact cgctacgctc ggtcgttcgg ctgcggcgag cggtactgac tcacacaaaa
     3061 acggtaacac agttatccac agaatcaggg gataagaccg gaaagaacat gtgagcaaca
     3121 aggcaaagcc aggacaaaag gccgcgccgc tggcgttttt ccataggctc cgcccccctg
     3181 acgagcatca caaaaaccga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     3241 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     3301 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     3361 gctgttggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     3421 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     3481 taaggcacgc cttaacgcca ctggcagcag ccactggtaa ctgatttaga ggagtttgtc
     3541 ttgaagttat gcacctgtta aggctaaact gaaagaacag attttggtga ctgcgctcct
     3601 ccaagccagt taccttggtt cacagagcgc caactctttg aaccttcgaa aaaccgcccg
     3661 ccaaggcggt tttttcgttt aacactctac aactatccat acgtgtatgg caacatccaa
     3721 tcaccactaa gtgtatgata gagatggata tgccaaatac aattcattac cctcatgtaa
     3781 taccatttat atcccaagga aaaataaatg ctataaaaag cacgtttggt aataacctgt
     3841 ctgataggga gtgctacggg atatatatat ggagccagaa agcctcatct gccatatatc
     3901 cattactgca acaactcgaa gttactttga gaaactcgat tgataaagaa gcgacaaaac
     3961 taataggcca aaaatggtgg gataatgtat acaccgacac atcaaaaagc aaacacggtg
     4021 acttcataca taatataaac aaagcaaaaa gaagatatga gaatgaattc aag
//
LOCUS       DAPHCV010000232         4062 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.236, whole genome shotgun sequence.
ACCESSION   DAPHCV010000232 DAPHCV010000000
VERSION     DAPHCV010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.236"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            48..1706
                     /gene="dpiB"
                     /locus_tag="RH033_004656"
     CDS             48..1706
                     /gene="dpiB"
                     /locus_tag="RH033_004656"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HDV2993717.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSIISAVKTRDYKRLATIADKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKPGALEKGESYFITGKGSIGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1675..2355
                     /gene="dpiA"
                     /locus_tag="RH033_004657"
                     /gene_synonym="citB"
     CDS             1675..2355
                     /gene="dpiA"
                     /locus_tag="RH033_004657"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HDV2993718.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2396..3781)
                     /gene="dcuC"
                     /locus_tag="RH033_004658"
     CDS             complement(2396..3781)
                     /gene="dcuC"
                     /locus_tag="RH033_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HDV2993719.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFIRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1111 a          954 c         1048 g          949 t
ORIGIN      
        1 ttaattacat ctgtcaaaga ggctcgggac aacccgcaag gaaaacaatg ttgcagctta
       61 acgagaataa acagtttgca tttttccaaa gactggcatt tccgctgcgt atctttttgc
      121 tgattctggt gttctcaata tttgtcattg cagccctggc gcaatatttt acagccagtt
      181 ttgaggacta tttaacgctt catgtgcgcg acatggcaat gaatcaggca aaaattattg
      241 cttctaatga cagtatcatc agtgcagtga aaacgcgtga ctacaagagg ctggcgacca
      301 tcgctgacaa attacaaaga gatactgatt tcgattatgt ggtgattggc gatcggcact
      361 cgattcgtct ttaccatcct aatccggaga aaattggtta tcctatgcag ttcaccaaac
      421 cgggcgcgct ggagaaaggg gagagctact tcatcaccgg gaaagggtca attggcatgg
      481 cgatgcgtgc caaaacgcca atctttgatg acgatggaaa agtcatcggc gtggtgtcga
      541 ttggctacct ggtgagtaaa atcgatagct ggcgggctga gtttttatta ccgatggctg
      601 gcgtgtttgt cgtgctgtta gggattctga tgttgctatc gtggttcctg gccgcgcata
      661 tccgtcggca gatgatgggc atggagccaa agcaaatcgc acgcgtggtc cgtcagcaag
      721 aggcgctgtt tagttcggtt tatgaagggc tcattgcggt ggatccgcat ggttacatta
      781 ccgccatcaa tcgtaacgca agaaagatgc tgggtctgag ttcccccgga cggcaatggt
      841 tgggtaaacc cattgctgaa gtggtcaggc ccgccgattt ctttaccgaa cagattgatg
      901 aaaaacgtca ggatgtggtg gcgaacttta acggtctgag cgttattgcc aaccgggaag
      961 ctattcgttc tggtgatgat ttgctggggg ccattatcag ctttcgtagt aaagacgaaa
     1021 tatccaccct caatgcgcaa ctgacgcaaa ttaaacaata cgtcgagagc ctgcgcacat
     1081 tgcgacacga gcatctcaat tggatgtcga cgctcaatgg tctgttgcag atgaaagagt
     1141 atgatcgcgt gctggcgatg gtgcaggggg agtctcaggc ccagcaacag cttattgaca
     1201 gcctgcgtga ggcgtttgcc gatcgccagg tggcggggct gctttttggt aaagtgcagc
     1261 gcgcccggga actggggcta aaaatgatca ttgtccccgg tagccagctt tcgcaactgc
     1321 cgccaggact ggatagcacc gagtttgcag ccattgtggg caatttactt gataacgcct
     1381 tcgaagccag cctgcgtagc gatgaaggaa acaagatcgt tgaattattc ctcagcgatg
     1441 aaggcgatga tgtggtgatt gaagtcgccg atcagggctg cggcgttcca gagtctctac
     1501 gagacaaaat atttgagcag ggggtcagta cgcgtgctga cgagcccggt gaacatggca
     1561 ttgggttgta cttgattgcc agctacgtaa cgcgctgcgg tggtgttatc actctcgaag
     1621 ataatgatcc ctgcggtacc ttgttttcaa tctatattcc gaaagtgaaa cctaatgaca
     1681 gctccattaa ccctattgat cgttgaggac gaaacgccgc tggcagagat gcatgcggaa
     1741 tatattcgtc acattcccgg attcagtcag atattactgg cgggaaacct ggcgcaggcc
     1801 cgaatgatga tcgagcgttt taagccgggg ctaatcttgc tcgataacta tcttcctgac
     1861 ggcagaggga ttaatttatt gcatgaactg gtgcaggcgc attatcccgg cgacgtggtg
     1921 tttaccactg cagccagcga tatggaaacg gtgtctgaag ccgtacgttg tggtgtattt
     1981 gattatctca ttaagcccat tgcctatgaa cggctggggc aaacgctaac ccgtttccgc
     2041 cagcgtaaac atatgctgga aagtattgat agcgccagcc agaagcaaat tgatgagatg
     2101 tttaatgcct atgctcgcgg tgaacctaaa gacgaactgc cgaccggcat tgatcccttg
     2161 acgctaaacg cagtgcgaaa actgtttaaa gagcctggtg tgcaacatac ggcagaaacg
     2221 gtggcgcagg cactgactat cagccgcacc actgccagac gttatcttga atattgcgcc
     2281 agccgccatc tgattattgc tgaaattgtt cacggcaaag ttggcagacc gcaacgcata
     2341 taccacagtg ggtgacatga taaagccgga ggaaacttgc ctccggcatg actatttact
     2401 tgcctgtgac cgcggctgcc gttcctggca ccatcagctc tgtagcaacg ataacaatca
     2461 ccagcccaac aagcaccggc accgaggtgc gttttacgac ttcaaatggt gagatcttcg
     2521 ccatcccggc aaccgcaacg actacgccag aaacgggtga cagggtacgg ccgaggtttg
     2581 acgcctgcag cattgggata gtcaaatacg ccgggttaat gccggaagag tgcgccagtt
     2641 tcgggatcat ctcaacaaac gcataaaacg gcgcattgcc tgaaccggtc gtgactgccg
     2701 ccagcattgt caggatcact aataccagca tcaggatgat actcgccgaa ccaaacgagg
     2761 tggcgataga aatcagactt tgaataaagc cgatggtgct aagcccctga gcgaataccc
     2821 cagcggcaac cagcagcatc accacgttag caaacgcatc ggccatcccg cgataagcca
     2881 cttccagacc agagaaaact ttctgggtat taaagctgcg gatgaactcc agaatggagg
     2941 caatcagcat acaaatcacc agaatagtga tgatgtgtaa ttgcggaccc catttgccgt
     3001 caaaaatcag cactccgatg atcggcgtga acggcaaaat ggcataaaac gcaggagcag
     3061 tggtggtgat ttcactgaca tctaacattt catgagagat gtgctctttt ttatccagat
     3121 aacgttgcca gaagaagtgg gcgatcgcca tgccgataat tgcagcaatt gagataggca
     3181 gcgtcgtttt gaaggcgaag tcaatcagcg acatttcgga agcctgcgcc gccagcacca
     3241 catcccctga agtcggtgcg agaataatcg ccgccgggga ggcacaaata gcagcagctg
     3301 caccacggct gataccaacg tttaccatca ccggaaacag ggttgccatc agcaaaacac
     3361 ccagaccggt cgcggaagag acggccagag acatcagaca ggcgacaaaa taggcggcaa
     3421 tcatcagcag gtaaggggag ttaatatact gcaatggttt tgacgccagc ttgaccacca
     3481 tatcattcgc gccgatatgg gtcatgtaag cggcaaagcc acacagcatc ataatcatca
     3541 tgccgaggtc gccgccgcgg ctcattagca atattttaac gtattcaacg atatccgtgg
     3601 cgctgtagcc tgttgaagcc tggctggacg gtaacacttt gtgccccata atggcactga
     3661 taatcagcaa taacaggcca ccgacaaata acacgccagt ggcggaatac cctttaatga
     3721 tgtagcgagc tacacccaca ataaccacaa ccccaataag gagttcaatg aatgtcagca
     3781 taatttttcc tgtctccagg ccccaaagta aataataaaa aattcctaaa gcttaaggaa
     3841 aaaatatgcc caataaattg gcgataaatg ctgattaaaa tcaagaaaaa ctgccattaa
     3901 gacattgaag ttgctgtttt tatacataga taacaaaacg catatttagc ggttggcaat
     3961 tatcacataa taatgtgtgt aaattgatgg tggattgatg caaatttgtt aattgcaatg
     4021 gtgttagctg cggctgtgcg ctcaaaaaat aatctaatat ga
//
LOCUS       DAPHCV010000233         4026 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.238, whole genome shotgun sequence.
ACCESSION   DAPHCV010000233 DAPHCV010000000
VERSION     DAPHCV010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.238"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(264..842)
                     /locus_tag="RH033_004659"
                     /pseudo
     CDS             complement(264..842)
                     /locus_tag="RH033_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001334685.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4238 domain-containing protein"
     gene            complement(879..1694)
                     /gene="sul2"
                     /locus_tag="RH033_004660"
     CDS             complement(879..1694)
                     /gene="sul2"
                     /locus_tag="RH033_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HDV2993720.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1909..2181
                     /locus_tag="RH033_004661"
     CDS             1909..2181
                     /locus_tag="RH033_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993721.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYQSVFTDFIAGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
     gene            2162..3370
                     /locus_tag="RH033_004662"
     CDS             2162..3370
                     /locus_tag="RH033_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HDV2993722.1"
                     /translation="MTRSGGDFQPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKML
                     ACGTRILGVKEYNCDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDW
                     VHLVFTLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGLEPGIFCAIHTYGRRLN
                     WHPHVHVSVTCGGLNKHGHWKKLSFLKDAMRSRWMWNMRQLLLKAWSEGLAMPESLSH
                     ITTESQWRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGA
                     SLSFRYLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQV
                     YRALGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVSGLKKNAR
                     DISLLRYMPA"
     gene            complement(3543..3800)
                     /locus_tag="RH033_004663"
                     /pseudo
     CDS             complement(3543..3800)
                     /locus_tag="RH033_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4238 domain-containing protein"
BASE COUNT          991 a         1058 c         1151 g          826 t
ORIGIN      
        1 atgggtcacg ccagtaaagc gcggactact ctggggtatc ggtaaagtgg ttaccgccac
       61 ttgccgaaga tttactctgc taaagtaagt agccgcaacg ctacacgaac tgatggtgaa
      121 tgtcaacaga tactcaccat ctccttacgg cggtggtccc tgtgaccact gccgctttcg
      181 cgtgggtgca acacggcaaa actcctctgt acaacagggg ctcccgccgt cattttccgg
      241 cacaggtgag gccggaattc ggactaaaac gtaaaccgcg ggccagtccg gtagcgttca
      301 ctatcggcca gcattctctc aaccagagag aaatcctttt caccgcagaa cacgtactgc
      361 tccgcgaact ccacctgcag ggaattgtaa tgcgtgacat gctccggggt aagcggcata
      421 agaagatagt ccgtatacct ccggatatgt tccagtcttt caaaaggccg aatgtgacgg
      481 gggataagat gcggcgccct ggcgagcaga tgttgaagat gctgcttttg acgaaccatc
      541 tgttcacgga tggaggggca atacatggcc agcatcagcg tcgatgacag ggggaggtaa
      601 atctgtatcc cgcgaacggc cagaccaaga ttaccgtacg gaccgaagtc attgctgttc
      661 ttaagcacta cgggattgtc tgatacgtaa aacggacgtt ctggcctggt ttccagaagg
      721 taccagtcct ttttcagcag gagatcaata tgtgacacct gctggacgag ctgccgcaga
      781 aaaatatcct tggtatcgcc cccggacgac aggcccaggg tttccatggc ctgctcttta
      841 gtgaaccgcg aatatagaga aatatcccga atgtgcagtt aacgaattct tgcggtttct
      901 ttcagcgccg ccaataccgc cagcccgtcg cgcaaggggc gcggctcgtg tgtgcggatg
      961 aagtcagctc cacctgcggc ggcggcaagc tctgcagcga gtgtcgcggc cccgacatcc
     1021 cccggaccac ggcctgtgag cgcgcgcaga aaggatttgc gcgaaacaga cagaagcacc
     1081 ggcaaatcga agcgcagccg caattcatcg aaccgcgcca gcaccgagag cgaggtttcg
     1141 ggagcagccc ccagaaaaaa ccccatgccg ggatcaagga caaggcggtt gcgtttgata
     1201 ccggcacccg tcagcgccgc gatgcgcgcg tcaaagaacg ccgcaatgtg atccatgatg
     1261 tcgccagcgg gtgcctcgcg ccgatctgcc tgcccgtctt gcaccgaatg cataacgacg
     1321 agtttggcag atgatttcgc caattgcgga tagaacgcag cgtctggaaa accgcgaata
     1381 tcattgagat aggccacacc acgcgacaag gcataggctt gcgtcgcggg ttgataactg
     1441 tcgagcgaga cgggaatgcc atctgccttg agcgcgtcca gcaccggcgc gatacgcgcg
     1501 atttctgtgt cggacgaaac aggcgcggcg tcgggattgc tggatgccgg accgaggtcg
     1561 atcacatctg ccccctcggc catcagctta cgcgcctgcg caatggctgc gtctggcgcc
     1621 agataccggc ctccatcgga gaaactgtcc gaggttatgt tgacgatgcc gaaaatgatg
     1681 agcgatttat tcatgggggc ttctctctgt acacgacaaa aaacaaaaaa gctgactaag
     1741 gacaaggcat aatagtaatt tttcttacga accacactat gccaaacctt aatcagctcg
     1801 taaatatact atcgcaaaac ctaaccatga accacggcag cctggcggct gcaaatcgtg
     1861 gtgtggcaga atatgagctt tcagagtaac catccagaat gagttcccat gtcacacaac
     1921 ctggagcacc agaaagtcca caccagaatg gtgaaagagg ttctgaaagc cgtcgccagg
     1981 gccaataatc acccttatca gtctgtattt acagacttta tcgccggtca tccctcctgt
     2041 acggtctgtt tctgggaaac cttccacaaa atgtacccgg attcaccgta cgaatacgtc
     2101 acgttctgcc atacctgccg tcgttttgat ttatacgaaa ctgaagcgga gatgaaggcg
     2161 gatgacccga agtggtggtg attttcagcc ccgaccgctg aaacgcctgt tcacagccaa
     2221 ccagtgctgg acatccttcc tggatgcggg cggcctgcgc gatatcgagg ttgaggctgt
     2281 cacaaaaatg ctggcctgcg gcacgcggat actgggtgtg aaggaataca actgcgataa
     2341 acctgagtgc ccccacgtca gatacgtcac taactcatgt ggcagccgtg cctgcccgtc
     2401 ctgcggaaaa aaggccacag acctgtggat agcgacacag ctgaatcgtc ttcctgactg
     2461 cgactgggtg cacctggtct tcaccctgcc ggacacgctg tggccggtgt tcgaaagcaa
     2521 ccgctggctg ctgaatgacg tgtgccgtct ggcggtggag aatctgctgt atgcagcccg
     2581 gaaacggggt ctggaacccg gtatcttctg cgccatccat acgtatggcc gtcgtctcaa
     2641 ctggcatccg catgtgcatg tgtcagtgac ctgcggaggt ctgaataaac acggacactg
     2701 gaaaaagctg agcttcctga aagacgcgat gcgttcgcgg tggatgtgga atatgcggca
     2761 gctgcttctg aaagcgtggt cagaaggtct ggcgatgccg gaatcgttgt cacatatcac
     2821 gacggaatca cagtggagaa gcctggtgct gaaagccggc ggaaaatact ggcatgtgta
     2881 catgtcgaag aagacagccg gagggcggaa tacggcacgc tacctgggtc gttatctgaa
     2941 gaagccacca atagcggctt cccggctggc gcattacaac ggaggagcga gtctgagctt
     3001 ccgttacctg gaccacaaaa cgggagaaac ggcgacggaa acgctgacac agcgggagct
     3061 ggtcgcgagg ctgaaacagc acatcccgga gaagtttttt aagatggtga gatacttcgg
     3121 gttccttgcc aaccgggtgt gtggagagaa gctgccgcag gtgtaccgtg ctctggggat
     3181 ggataagccg gaaccagtgg cgaaagtgtg ctatgcacaa atggtgaaac agttcctgag
     3241 tcgtgacccg ttcgaatgcg tgctgtgtgg cggccggatg gtataccgcc gggccattgc
     3301 ggggctgaat gtatcagggc tgaagaaaaa cgcgcgggat atcagtctgc tgaggtatat
     3361 gccggcctga cacaggctaa gtgtgcccgg gaatcggtgc aggtataaaa aacagcctgt
     3421 tacggtataa aaatcgacca tgcagagaga gaaaccaccg gagtggaggg aaaagcagaa
     3481 aggctcacgt ggcataaggc gtggacagcg gtatcgtctg gtttttcaat ttcctatacg
     3541 tttaccggtg ccgcgtggtg ctcgtaaatc cccagcagtg gttcaagact cagacggtcc
     3601 gggtgattat cgaggttgta gaacgtgttg cggacggtcg cgtcatccgg cgtggtggca
     3661 taggcgcgcc cggcagcctt gtcaaaggcg tataagcgct gccgcttacc ctttcccggc
     3721 gtactgaaat gcttcagtaa aaaacgggga accgtatgct gcctgaccac atcggtctgc
     3781 agattgaatg gatcggccat aactttatct ccctgaaaag ccacgcaagc gcatcggtgc
     3841 atctgtattg acggcatggg cagctggttt gcagcttagc attagaaaac agaaccgttc
     3901 gataatacct gtgtttaacg ccctgtcagt accgatggaa tttaaatctc cggaaccgcc
     3961 ggaaaccgga tctgcacgac gggaaacggg caactgatat gataatttag caatacacat
     4021 aaaatc
//
LOCUS       DAPHCV010000234         3837 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.239, whole genome shotgun sequence.
ACCESSION   DAPHCV010000234 DAPHCV010000000
VERSION     DAPHCV010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.239"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            227..982
                     /gene="repE"
                     /locus_tag="RH033_004664"
     CDS             227..982
                     /gene="repE"
                     /locus_tag="RH033_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepE"
                     /protein_id="HDV2993723.1"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVD
                     QIRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEED
                     AGDEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNP
                     YAMRLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINS
                     RTPMRLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     gene            1570..2736
                     /gene="sopA"
                     /locus_tag="RH033_004665"
     CDS             1570..2736
                     /gene="sopA"
                     /locus_tag="RH033_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HDV2993724.1"
                     /translation="MKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPT
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            2736..3707
                     /locus_tag="RH033_004666"
     CDS             2736..3707
                     /locus_tag="RH033_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HDV2993725.1"
                     /translation="MKRAPVIPKHTLNTQPVGDTSLSTPAAPMVDSLIARVGVMARGN
                     AITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA
                     RSGDALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLS
                     SRHQFAPGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
BASE COUNT          975 a          834 c          914 g         1114 t
ORIGIN      
        1 taggccttta tccatgctgg ttctagagaa ggtgttgtga caaattgccc tttcagtgtg
       61 acaaattacc ctcaaatgac agtcctgtct gtgacaaatt gcccttaacc ctgtgacaga
      121 ttgccctcag aagaagctgt tttttcacaa agttatccct gcttattgac tcttttttat
      181 ttagtgtgac aatctaaaaa cttgtcacac ttcacatgga tctgtcatgg cggaaacagc
      241 ggttatcaat cacaagaaac gtaaaaatag cccgcgaatc gtccagtcaa acgacctcac
      301 tgaggcggca tatagtctct cccgggatca aaaacgtatg ctgtatctgt tcgttgacca
      361 gatcagaaaa tctgatggca ccctacagga acatgacggt atctgcgaga tccatgttgc
      421 taaatatgct gaaatattcg gattgacctc tgcggaagcc agtaaggata tacggcaggc
      481 attgaagagt ttcgcgggga aggaagtggt tttttatcgc cctgaagagg atgccggcga
      541 tgaaaaaggc tatgaatctt ttccctggtt tatcaaacgt gcgcacagtc catccagagg
      601 gctttacagt gtacatatca acccatatct cattcccttc tttatcgggt tacagaaccg
      661 gtttacgcag tttcggctta gtgaaacaaa agaaatcacc aatccgtatg ccatgcgttt
      721 atacgaatcc ctgtgtcagt atcgtaagcc ggatggctca ggcatcgtct ctctgaaaat
      781 cgactggatc atagagcgtt accagctgcc tcaaagttac cagcgtatgc ctgacttccg
      841 ccgccgcttc ctgcaggtct gtgttaatga gatcaacagc agaactccaa tgcgcctctc
      901 atacattgag aaaaagaaag gccgccagac gactcatatc gtattttcct tccgcgatat
      961 cacttccatg acgacaggat agtctgaggg ttatctgtca cagatttgag ggtggttcgt
     1021 cacatttgtt ctgacctact gagggtaatt tgtcacagtt ttgctgtttc cttcagcctg
     1081 catggatttt ctcatacttt ttgaactgta atttttaagg aagccaaatt tgagggcagt
     1141 ttgtcacagt tgatttcctt ctctttccct tcgtcatgtg acctgatatc gggggttagt
     1201 tcgtcatcat tgatgagggt tgattatcac agtttattac tctgaattgg ctatccgcgt
     1261 gtgtacctct acctggagtt tttcccacgg tggatatttc ttcttgcgct gagcgtaaga
     1321 gctatctgac agaacagttc ttctttgctt cctcgccagt tcgctcgcta tgctcggtta
     1381 cacggctgcg gcgagcgcta gtgataataa gtgactgagg tatgtgctct tcttatctcc
     1441 ttttgtagtg ttgctcttat tttaaacaac tttgcggttt tttgatgact ttgcgatttt
     1501 gttgttgctt tgcagtaaat tgcaagattt aataaaaaaa cgcaaagcaa tgattaaagg
     1561 atgttcagaa tgaaactcat ggaaacactt aaccagtgca taaacgctgg tcatgaaatg
     1621 acgaaggcta tcgccattgc acagtttaat gatgacagcc cggaagcgag gaaaataacc
     1681 cggcgctgga gaataggtga agcagcggat ttagttgggg tttcttctca ggctatcaga
     1741 gatgccgaga aagcagggcg actaccgcac ccggatatgg aaattcgagg acgggttgag
     1801 caacgtgttg gttatacaat tgaacaaatt aatcatatgc gtgatgtgtt tggtacgcga
     1861 ttgcgacgtg ctgaagacgt atttccaccg gtgatcgggg ttgctgccca taaaggtggc
     1921 gtttacaaaa cctcagtttc tgttcatctt gctcaggatc tggctctgaa ggggctacgt
     1981 gttttgctcg tggaaggtaa cgacccccag ggaacagcct caatgtatca cggatgggta
     2041 ccagatcttc atattcatgc agaagacact ctcctgcctt tctatcttgg ggaaaaggac
     2101 gatgtcactt atgcaataaa gcccacttgc tggcctgggc ttgacattat tccttcctgt
     2161 ctggctctgc accgtattga aactgagtta atgggcaaat ttgatgaagg taaactgccc
     2221 accgatccac acctgatgct ccgactggcc attgaaactg ttgctcatga ctatgatgtc
     2281 atagttattg acagcgcgcc taacctgggt atcggcacga ttaatgtcgt atgtgctgct
     2341 gatgtgctga ttgttcccac gcctgctgag ttgtttgact acacctccgc actgcagttt
     2401 ttcgatatgc ttcgtgatct gctcaagaac gttgatctta aagggttcga gcctgatgta
     2461 cgtattttgc ttaccaaata cagcaatagt aatggctctc agtccccgtg gatggaggag
     2521 caaattcggg atgcctgggg aagcatggtt ctaaaaaatg ttgtacgtga aacggatgaa
     2581 gttggtaaag gtcagatccg gatgagaact gtttttgaac aggccattga tcaacgctct
     2641 tcaactggtg cctggagaaa tgctctttct atttgggaac ctgtctgcaa tgaaattttc
     2701 gatcgtctga ttaaaccacg ctgggagatt agataatgaa gcgtgcgcct gttattccaa
     2761 aacatacgct caatactcaa ccggttggag atacttcgtt atcgacacca gctgccccga
     2821 tggtggattc gttaattgcg cgcgtaggag taatggctcg cggtaatgcc attactttgc
     2881 ctgtatgtgg tcgggatgtg aagtttactc ttgaagtgct ccggggtgat agtgttgaga
     2941 agacctctcg ggtatggtca ggtaatgaac gtgaccagga gctgcttact gaggacgcac
     3001 tggatgatct catcccttct tttctactga ctggtcaaca gacaccggcg ttcggtcgaa
     3061 gagtatctgg tgtcatagaa attgccgatg ggagtcgccg tcgtaaagct gctgcactta
     3121 ccgaaagtga ttatcgtgtt ctggttggcg agctggatga tgagcagatg gctgcattat
     3181 ccagattggg taacgattat cgcccaacaa gtgcttatga acgtggtcag cgttatgcaa
     3241 gccgattgca gaatgaattt gctggaaata tttctgcgct ggctgatgcg gaaaatattt
     3301 cacgtaagat tattacccgc tgtatcaaca cagccaaatt gcctaaatca gttgttgctc
     3361 ttttttctca ccccggtgaa ctatctgccc ggtcaggtga tgcacttcaa aaagccttta
     3421 cagataaaga ggaattactt aagcagcagg catctaacct tcatgagcag aaaaaagctg
     3481 gggtgatatt tgaagctgaa gaagttatca ctcttttaac ttctgtgctt aaaacgtcat
     3541 ctgcatcaag aactagttta agctcacgac atcagtttgc tcctggagcg acagtattgt
     3601 ataagggcga taaaatggtg cttaacctgg acaggtctcg tgttccaact gagtgtatag
     3661 agaaaattga ggccattctt aaggaacttg aaaagccagc accctgatgc gaccacgttt
     3721 tagtctacgt ttatctgtct ttacttaatg tcctttgtta caggccagaa agcataactg
     3781 gcctgaatat tctctctggg cccactgttc cacttgtatc gtcggtctga taatcag
//
LOCUS       DAPHCV010000235         3829 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.240, whole genome shotgun sequence.
ACCESSION   DAPHCV010000235 DAPHCV010000000
VERSION     DAPHCV010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.240"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            19..>3829
                     /locus_tag="RH033_004667"
     CDS             19..>3829
                     /locus_tag="RH033_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX toxin"
                     /protein_id="HDV2993726.1"
                     /translation="MKMIFTGKVSGEKTVLTVGGRHTVKAQPGEQYGLIDEVTGLVPD
                     GVEADRSGDDLILRKKEEDTEVRIEGFWEECQPGETQCTAVFNIVGEDGQVTEAVLTQ
                     DGPVLDDITAGQSGTLSDDDRGGFIWLGGLAFGGGLAAMALAAGGGGSKHRHENDDSD
                     TTAPSSPALKAEDDGSVSVELPGDANKGDTVDVTFEDEKGGKHTVTLEKGDNGWTSSD
                     PTLIPDSTGDKATIPADNVKDNSEVTGVAKDPSGNESDPSTVTSKTDGVADAPVLSIP
                     EVTDGYANADELKDGLQAEVTLPAGTAEGAVITLTVTRPDKTTENVTHTVTKDEVAAG
                     KVSMDIPKDAVIDGQNSVSVTLTQGSNPAKPGNVVDFAADTQIPGDTDGDGATDATPV
                     VAIPEAADGVNAEELKDGVQTEVTVPKGSAAGDTLTLTVTKPDGTTDTVEHTLTADEV
                     AAGKADVTIPADKVTADGQYSVTAEITDPAGNTSGQGQPADFAVDTVAPSAPVLKAED
                     DGSVSVELPGDANKGDTVDVTFEDEKGGKHTVTLEKGDNGWTSSDPTLIPDSTGDKAT
                     IPADNVKDNSEVTGVAKDPSGNESDPSTVTSKTDVLPTVSISVETTSTDVNGDGFTGI
                     ASVNGTVMDVPATIEDKDDSTGLVYTVSLNHVTTTDVTVTVTLGSGAGHSDAADYSDI
                     GGAQHNGKIGLHGDTGKVTYDGATTVTIVIPAGSKSVSFIVDPTLEANQDAFNAEGME
                     KVVATITGTSDNVTAATDIVDNAGASATGVIYDGNAISLRNLDGDFTLKYSLSSSIAE
                     KGDFGYTIGANSGENDPMVTTDYNDTVYVGYYQSGKETTSYSNVANSQDNGPDGTKTD
                     GNQSITTVDLGAGDDLMVIRGNMLANTRVYTGEGNDTFTMDGMNTALRVMYAGSYIFT
                     ESGDDIVTIKRTGVTNAGQIYLGSGSDTFIQGDATDNNDTTLSGLLDLGSGTKDISNM
                     PKEYLSVYQDGSNLSLGNDNNIDTATDVNTVTIYGSVSGEILGGYGSDNITVTKNLTG
                     NISVGDNADTLTAGWIYGGSTVSMGDGNDTVTITDGAYNTTISLGAGDDVFDATSGVM
                     GDSAYATVVNGEDGNDTFKLGTIAKNLTIDAGAGDDIVVLTKDYDSTSSGNQGYINGG
                     EGSDTLVLTGTISVNLAAGKNEGIAGFEKVDMTVGSDLKADNAAQLVKLTASDVLGIN
                     DNSTIYISGDANDKVDLGADGAGSLGTFTATATTVKATALDGIEHTYTLYSSVSGANV
                     YIDNNIID"
BASE COUNT         1071 a          738 c         1071 g          949 t
ORIGIN      
        1 ttagcctaag gattttatat gaaaatgatt tttaccggta aggtcagcgg agaaaaaacc
       61 gtgctgactg tcggaggtcg tcatacagtt aaagcacagc ccggggagca gtatggactg
      121 atcgatgagg tgaccggcct agtgcctgac ggtgttgagg cggatcgctc cggtgatgat
      181 ttaattctgc gtaaaaagga agaagatact gaggtccgga tagagggatt ctgggaagaa
      241 tgccagcccg gggaaacaca gtgtacggcg gtatttaata ttgttgggga agatggacag
      301 gtcaccgaag ccgtactgac tcaggatggc ccggtgctgg atgatattac tgccggacaa
      361 tcgggaacct tgtcagatga tgatcgtggc ggttttatat ggttgggcgg tctggcattt
      421 ggtgggggac tggctgctat ggctttagct gcaggtggcg gtggaagcaa acatagacat
      481 gaaaatgatg actctgatac tacagcccca tcgtcaccag cactgaaggc agaggatgac
      541 ggttctgttt ctgttgagct gccgggtgat gccaataaag gcgataccgt agatgtcacc
      601 tttgaagatg aaaaaggtgg taagcacacc gtgacgctgg agaaagggga taacggctgg
      661 acatccagtg atccgacgct gatcccggac agcactggcg ataaagcgac cattccggca
      721 gataacgtga aggataacag tgaagttacc ggggttgcca aagatccgag cggtaatgaa
      781 agtgacccgt caaccgtgac gtcaaaaact gatggtgtgg cagatgcccc ggttctgagc
      841 attcctgaag tgactgacgg ttatgccaat gctgatgagc tgaaagacgg tttgcaggca
      901 gaggtcacgt tgccggcagg cacggcagaa ggtgcagtta ttacacttac cgtgacccgt
      961 ccggacaaaa ccacggagaa cgtgacacat accgtgacta aagatgaagt ggctgccggt
     1021 aaagtatcca tggatattcc gaaggatgcg gtgattgacg ggcagaacag cgtcagtgtc
     1081 actctgactc agggcagtaa cccggcgaaa cccgggaatg tggttgattt tgctgccgat
     1141 acccagatcc cgggggatac cgatggtgat ggtgcaacag atgcaacacc ggtagttgcc
     1201 attccggaag cggcagatgg tgtgaatgcc gaagagctga aggatggtgt acagaccgaa
     1261 gtcacggtac cgaaaggcag cgctgccggt gataccctga cgctgacggt caccaaaccg
     1321 gacggcacga cggacacggt ggagcacacg ctgaccgcag atgaagtggc tgcaggtaaa
     1381 gctgatgtga ccattccggc ggataaagtg acggcagatg gtcagtattc tgtaaccgcg
     1441 gaaatcacag acccggcagg taacaccagt ggccagggcc agccggctga cttcgcagtg
     1501 gatacagtgg cgccgtcggc accggtatta aaagcagagg atgatggttc tgtttctgtt
     1561 gagctgccgg gtgatgccaa taaaggcgat accgtggatg tcacctttga agatgaaaaa
     1621 ggtggtaagc acaccgtgac gctggagaaa ggggataacg gctggacatc cagtgatccg
     1681 acgctgatcc cggacagcac tggcgataaa gcgaccattc cggcagataa cgtgaaggat
     1741 aacagtgaag ttaccggggt tgccaaagat ccgagcggta atgaaagtga cccgtcaacc
     1801 gtaacctcaa aaactgatgt gttaccaaca gtgagtatta gcgttgagac aacatcaaca
     1861 gatgtcaatg gtgatggttt tactggtata gctagtgtca atgggaccgt aatggatgtt
     1921 cccgcaacta ttgaagataa ggatgattcc actggactgg tttatacggt ttctctgaat
     1981 catgtcacta caacagatgt aacagttaca gttacattgg gtagcggagc tgggcattct
     2041 gatgctgctg actattctga tattggtgga gcacagcata acgggaaaat tggccttcat
     2101 ggtgatactg gaaaagtaac ctatgatggt gcaacaaccg taacaattgt tattcctgcc
     2161 ggttcaaaaa gtgtttcatt tatagttgat ccaacgcttg aggctaacca agatgccttt
     2221 aatgccgagg gtatggaaaa agttgttgcg accattacgg gaacatctga taacgttacg
     2281 gcagctactg acatcgttga taatgcggga gcatccgcaa caggtgttat ttatgatggt
     2341 aatgccattt cattacgtaa tctggacggt gattttacac tgaaatattc actatcatct
     2401 tctatcgccg aaaaagggga ctttgggtat accattggcg cgaatagtgg tgaaaatgat
     2461 ccaatggtaa caacagatta caatgatacg gtttatgttg gttattacca gagtggtaaa
     2521 gaaacaacat cgtatagtaa tgttgctaac tcacaggata atgggccaga cggaacaaaa
     2581 accgatggca atcaaagcat tacgactgtt gatttaggtg ctggtgatga tctaatggtc
     2641 attcgtggta acatgctggc taatacaaga gtatatactg gtgagggtaa cgatactttt
     2701 actatggatg gtatgaatac tgcattacgt gttatgtatg cgggtagtta tatctttact
     2761 gagtctggcg atgatattgt tacgatcaaa cgtactggtg taacaaatgc cggacagatt
     2821 tatcttggtt caggtagtga tacatttatc cagggggatg ctactgacaa taacgataca
     2881 acattatctg gtttattgga tcttggtagt ggtacaaaag atatatctaa tatgcctaag
     2941 gaatatcttt ctgtttatca ggatggcagt aatttaagct tgggtaacga caacaatatt
     3001 gacactgcca cagatgttaa taccgtcact atttatggct ctgtgtctgg tgaaatttta
     3061 ggcggatacg gcagtgataa tattacggta actaaaaatc tgaccggtaa tatttctgtc
     3121 ggtgacaatg cggatacctt aacggccgga tggatttatg gtggcagtac tgtctctatg
     3181 ggggacggca atgatactgt taccatcaca gatggtgcat ataacacgac tatttcactg
     3241 ggggcgggag atgatgtatt tgatgcaact tctggtgtaa tgggggattc tgcttatgcc
     3301 actgtcgtta atggtgaaga cggcaatgat acctttaaat taggcacaat tgccaaaaac
     3361 ctgaccattg atgccggtgc cggtgatgat attgttgttc tgacaaaaga ctatgactcc
     3421 acctcctccg gtaatcaggg ctatattaac ggcggggaag gcagtgatac cctggttctg
     3481 acaggtacca tttctgtcaa tctagcggcc ggtaaaaatg aaggtattgc cggcttcgag
     3541 aaagtggata tgactgttgg tagcgatctg aaagctgaca atgcagctca gttagtcaag
     3601 ttaacggcat cggatgtatt gggtataaat gacaattcaa cgatttatat ttccggtgat
     3661 gccaatgaca aagttgattt gggggctgac ggagcgggaa gtctgggtac atttacggca
     3721 acggcaacaa ctgttaaagc aactgcgtta gatggtattg agcatactta tacgttatac
     3781 agcagtgtaa gtggcgcaaa tgtgtatatt gataacaata ttattgatg
//
LOCUS       DAPHCV010000236         3821 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.241, whole genome shotgun sequence.
ACCESSION   DAPHCV010000236 DAPHCV010000000
VERSION     DAPHCV010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.241"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            151..1389
                     /gene="alaC"
                     /locus_tag="RH033_004668"
     CDS             151..1389
                     /gene="alaC"
                     /locus_tag="RH033_004668"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HDV2993727.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYGIEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            complement(1454..1525)
                     /gene="ypdK"
                     /locus_tag="RH033_004669"
     CDS             complement(1454..1525)
                     /gene="ypdK"
                     /locus_tag="RH033_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HDV2993728.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(1881..2801)
                     /gene="lpxP"
                     /locus_tag="RH033_004670"
     CDS             complement(1881..2801)
                     /gene="lpxP"
                     /locus_tag="RH033_004670"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HDV2993729.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLYITPEMEGYPADENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            3154..3396
                     /locus_tag="RH033_004671"
     CDS             3154..3396
                     /locus_tag="RH033_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HDV2993730.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            complement(3473..3748)
                     /locus_tag="RH033_004672"
     CDS             complement(3473..3748)
                     /locus_tag="RH033_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993731.1"
                     /translation="MKVNLMIFSLFLLVSIMACNVFAFSISGGRSETSYKETEKTSVM
                     TTTHSTKLQPSQAILFKLREDAPPLNLTEDITPPFSTKANYLIHPVR"
BASE COUNT          980 a          893 c          960 g          988 t
ORIGIN      
        1 ttttaaagca gagttcctca tttaacgtca ttaataaaaa tgttctaaaa cgctggacag
       61 acagaaatta atcaggctat tttcaaaaca gccaccatcg tggcagcgtc gctcggacgg
      121 tccgggcgct aacgttaatc tgaggaaatt atggctgaca ctcgccctga acgtcgcttt
      181 acgcgcattg atcgtctccc gccctatgtt tttaacatta ccgctgaact gaaaatggct
      241 gcgcgtcggc gcggcgaaga tattattgat ttcagtatgg gtaacccgga cggtgcgact
      301 ccgccgcata tcgtcgaaaa attatgtacg gtggcccagc gcccggacac gcatggttac
      361 tccacgtcac gcggcattcc gcggttacgt cgcgccattt cccgctggta tcaggatcgc
      421 tacggcattg agatcgaccc ggaatcagaa gccatcgtca ctattggttc gaaagaggga
      481 ctggcgcatc tgatgctggc gacgctggat catggtgaca ctgtactggt gcctaatcca
      541 agctacccga ttcatattta cggcgcggtg attgccgggg cgcaggtacg ctcagtgccg
      601 ctggtggaag gtgtcgattt cttcaacgaa ctggaacgcg ccattcgcga aagttatccg
      661 aaaccgaaga tgatgatcct cggcttcccg tcgaatccaa ccgcgcaatg cgtggagctg
      721 gagttcttcg aaaaggttgt ggcgctggcg aaacgctacg atgtgctggt ggtccatgac
      781 ctggcctatg ccgatatcgt ctacgatggc tggaaagcgc cgtcaatcat gcaggtgcca
      841 ggtgcacgcg atgtggcggt cgagttcttt acgctgtcga aaagctacaa catggcgggc
      901 tggcgtatcg gctttatggt tggcaacaaa acgctggtca gcgcgctggc acgtattaaa
      961 agctatcacg attacggcac ctttacgccg ttgcaggtgg cagcgattgc ggcgctggag
     1021 ggcgatcaac agtgcgtgcg cgacattgct gaacagtaca aacgccgccg tgatgtactg
     1081 gtaaaagggc tgcatgaagc gggctggatg gtcgaaatgc cgaaggcttc gatgtatgtc
     1141 tgggcgaaaa tcccggaacc gtatgctgcc atgggctcgc tggaatttgc caagaagctg
     1201 ctgaacgaag cgaaggtctg tgtctctccg gggattggct ttggcgacta cggcgacacg
     1261 catgttcgct ttgcactgat tgaaaaccgc gaccgtattc gtcaggcgat tcgtggaatt
     1321 aaagcgatgt tccgtgccga cggtttacta cccgccagca gcaaacatat tcacgaaaac
     1381 gcggaataaa caccatcgcc agaaatgcaa acaggagcca gatggctcct gtttttcatt
     1441 gcacagtttg cttttagatc atcagggcaa aagtaccggc ccaaacgatg accataaaag
     1501 aaatgcccat aaagaaatat ttcactttac atctctccgc gtaattactg ttacgcctga
     1561 atgacttcat tacacctgat acagtgagtt gacaccgggc aaaacgacct taaaagtcct
     1621 gtattttacc gtgggcatca ccccagaatt ctgagctaaa ctgctccaga cggcgctaat
     1681 gtacgcctaa aactgaaacg agacaagagg cattttctta tttactttta gtaacttaca
     1741 agtgtctcat atcggcgaca gtttaatttc tgacggtaat aattagagat tcagcgaagg
     1801 aaaaaacctt aagaaaaaag ccataaaaac catgaggtta ttatggctga tttgagaagg
     1861 gaaagagtaa gagcagtttg ttaaatgtat agcgacgatt ctcccaccgg gcgcgtttta
     1921 aagcgacggt ggatccagag gtactgctcc ggtgcgcgca tgatctcttt ttcgataatc
     1981 ttgttcatat aggcagcggc ttgattttca tctgccgggt agccttccat ctctggggtg
     2041 atgtacaaac gatatccgct gtaatctgct tttcttacca tcgttacggt taacatggct
     2101 gcgccagaga gacgtgaaag gacatacgta ccattggttg tggcgacatt ttccaccgca
     2161 aagaacggcg cgaaggagct gcctttacga ccataatcct gatcgggagc aaaccatacc
     2221 gcttcacctt tcttcagtgc accgacaatg ccgcgcagat tatttctgcc gatcatcgct
     2281 ttgttagagc gcatgcgccc acgggtctgc acccattcca tcagctgatt attatgtgga
     2341 cgataggtag ccatcattgg ttggcacagt cccatcacgc ggccgcccag ttccagcgac
     2401 ataaaatgga cgccgacaac cattacgccg cgattttgca tttgtgcgcg tttaaggtta
     2461 tccaaccctt caacatcaaa ccatttacgt acgcgactgt cgggccagaa ccacgccatg
     2521 ccggtttcta ccagcgccat gccgagtgaa cgaaagtttt cggcaatcat cttctcgcgt
     2581 tcttccgcag aatgctgcgg gaaacaaagt tccaggtttt tacgggcgat agattcacgc
     2641 cgtttcagga atggtcgcgc cattgcgcca atacgcgtgc cgagaaagca gagaacagga
     2701 taaggaagct gtacccaaag ccagagtaca ccaagcccaa accatgtgag ccagtagcga
     2761 ggatgtagaa actcgcggga aaatttgcat tgtggaaaca tagttacctt ctgtagatac
     2821 gaattgttgt atcgcagaag attccatgtc gacagcggta cactcaattg gttgggccat
     2881 catgtacgac aatggttcct gtaaagagtg taaagactag tcaggacata accacgagga
     2941 gcatgaatgc ggtggctcta ctttatcact ggctgactgc tttcagctac tgattattaa
     3001 tcgtgcaaag taaaaaattt tccgcacgca atagcgttgc atttaacgtg ctgttttcat
     3061 tgatgcttag caaaaattgc cagaggttaa ggattgtctt actattaaag tgactgaagt
     3121 tacaggtttt ttagcgcggc aggaggttga gctatgattt atttatggat gtttctcgcc
     3181 ctgtgtattg tctgcgtaag cggctacatc ggtcaggtac tgaacgtggt atcggcggtc
     3241 tcttctttct tcggcatggt gatcctcgct gcactcattt attacttcac catgtggtta
     3301 acaggcggta atgaactggt gacggggata tttatgtttc ttgccccggc ttgtggcttg
     3361 atgattcgct ttatggtggg gtatggcagg cgctagtggg tcaggatggc tcatttgggc
     3421 aggtgttcac atcagcatca ggcatgattg ttcacctgcc atgtgacaga cgctatcgca
     3481 caggatgaat aagataattc gcctttgtcg aaaaaggggg cgtaatgtct tctgtgagat
     3541 ttaatggtgg cgcatcttct ctcaacttaa acaaaatcgc ctgtgatggc tgaagttttg
     3601 tagaatgagt tgtcgtcatc actgacgttt tttctgtctc tttatagctc gtctcacttc
     3661 taccaccaga aatggaaaat gcaaaaacat tgcatgccat aatagagacc aataaaaata
     3721 agctaaaaat cattaagttt actttcatag aaagttatcc tgaaaatggt tcactgagaa
     3781 cctacgatgt tatggctgta tttacattta attaatcagt a
//
LOCUS       DAPHCV010000237         3817 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.242, whole genome shotgun sequence.
ACCESSION   DAPHCV010000237 DAPHCV010000000
VERSION     DAPHCV010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.242"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(196..627)
                     /locus_tag="RH033_004673"
     CDS             complement(196..627)
                     /locus_tag="RH033_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tolA family protein"
                     /protein_id="HDV2993732.1"
                     /translation="MVLRSIWIEEQYMGVKKIVMLAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(695..3406)
                     /locus_tag="RH033_004674"
     CDS             complement(695..3406)
                     /locus_tag="RH033_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDV2993733.1"
                     /translation="MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDV
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCNSLETAAEEMQY
                     KADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINWLSWSQEKLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYEP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLAKAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNLGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGYTTVQCESLIEDMIAGGNF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAGADIFERVKPECRYNPELAASDEVEPGFRRDPVLRRERREARAAAREDLRARYLVW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAAQGDRAAVSQLRGWDYRDRRKDKRRTTNA
                     DRCVILCEPGGTPLYEDTGVLEARLQKDGSVRFRDRRNGELVCVDYGDRVVFYHHQDR
                     NELVDKLNLIAPVLFDREPGMGFEPEGSYQQFNDVFAEMVAWHNAAGITGNGHFVISR
                     PDVDLHRQRSEQYYHEYIRQQKSISGGHGASYAPVQDNEWTPPSPGM"
     gene            complement(3418..3750)
                     /locus_tag="RH033_004675"
     CDS             complement(3418..3750)
                     /locus_tag="RH033_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HDV2993734.1"
                     /translation="MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVST
                     FIRSSSLDKVVNCRTDDRMIDEIMRLGRLQKHLFVEGKRTGDKEYAEVLVAITEYVNA
                     LRRDLMGR"
BASE COUNT          923 a         1100 c          794 g         1000 t
ORIGIN      
        1 tcacagacag aaagaacgag ggtaccaata ccattgatgg taactggcag gccgatgaag
       61 gacgccgtta ccgggataat gtaagaatct atttttgagt atgtcaacat aacctgaggc
      121 aaagtacctc aggttccagt gtagtaatca gccaacctga cagtcttctg ttagccctac
      181 agatatgtcg tggagctaac cgcgcatgtc aaagccggat ttttgaaaat ctctataaac
      241 ttccggattg ttgaaggccg ggaatttagc tttatgggct gctgacttta tcgcttcgca
      301 ataggcttta tcgccattac tggttgatat ttttaacgcc gtgccatccc gggagaattc
      361 catatgcaac ctgcattttt tccttttcca tttttgcgga tcatcaagtt tagcattaat
      421 tgcatccctg attccccgtg cctgctcccc ccattcatcc tgatcatccc aacgtcctga
      481 actgcaacta cccagggctg tagttttatg gcaatttgaa ggatgtaatg gcgcacatcc
      541 cgccacaaag ctgactaaaa tagccagcat aacgattttc tttaccccca tatattgctc
      601 ctcaatccat atagatctca gtaccatagc tctgtcagag caaagaaaat cttaactaat
      661 acaagttaag atttttaata tccactcctc atccctacat tccaggtgat ggtggcgtcc
      721 actcattatc ctgaactgga gcataagatg cgccatgccc cccggatatg cttttctgtt
      781 gcctgatata ttcgtggtag tactgttcac tacgctgacg atgtaaatcc acatccgggc
      841 ggctgattac aaagtgtcca tttccggtta ttccagctgc attatgccag gccaccattt
      901 ccgcaaatac atcattaaac tgttgatatg agccttccgg ttcaaaaccc attcctggct
      961 cacgatcaaa caatacagga gcaatcagat tcagcttatc caccagttca tttctgtcct
     1021 gatgatggta gaacaccaca cggtccccat aatccacaca gactaactcg ccattcctcc
     1081 gatcacgaaa acgcacactg ccatctttct gcagacgggc ttcgagaacg ccagtgtctt
     1141 cataaagtgg agttcctccc ggttcacaga gaatcacgca gcggtcggca ttcgttgttc
     1201 gtcgcttgtc tttgcgacga tcacgataat cccagccacg taactgcgat actgcagccc
     1261 tgtctccctg agccgcctgt tgttccaccc actgccggta ggacaaagga taccacttac
     1321 cctccgcaat cagtgacaac cgctcttcct tcacactctc cttcagcctg atcagcgcct
     1381 gcatacgctg cacttccgca atatggtaat gcagcttacg caattgtgga tcccggaact
     1441 gcacacggat atacgccttg cgccgacggc aggcagcatg aatttcccgt aaccgttctc
     1501 cgtaacgtaa gtcgggttta cgccagtgct ccttccatac aagataacgc gcgcgcaaat
     1561 cttcccgcgc agctgcacgg gcttcacggc gttcacgacg cagtacagga tcgcgccgga
     1621 agccgggctc cacttcatcg ctggcagcaa gttccggatt gtagcgacat tcaggcttca
     1681 cccgttcaaa tatatctgca cctgcaatct caaatggccc agcctgcggc tcagccctgg
     1741 ataacgtcaa atccgggtgg atactgctgg ccttcactgg tgtgagctca tgattaaacg
     1801 catccacaat cacaagacca tgatgctgtt tctgcaacat aagcccttta cgcgcaaaca
     1861 tctcatggca cgcctgccag ctgaaattcc cgcccgcgat catatcttca atcaacgact
     1921 cacactgaac agtcgtgtac ccccacagac tttccttatc agaaaacagt tcacgcttta
     1981 ttgctccctg tggacctgtc cccatccgca tacgatacca ttcgtaatca ctgacagcct
     2041 gagtaccaag attacgtccc cggaatcgtt ctccgggtat aacctgagta aaaatatcct
     2101 tcggtacagg ctgccatcca ccatccagat cctgcatata atccagcgtc attaacggcc
     2161 acacactgtc tgcacgtact ggtgtctgtt gtttatcaaa tccatcatgc agtaccagat
     2221 gcccgtgctg gatccccatc cataatccag cctttgccag agttctgtgc acatccaggc
     2281 aactttccag ctcaccattt ctcgccattt ccggtaagcg ttcagcaaag tgacggcagg
     2341 cgtactgttt cagactctcc gtttctgcca ctttttccgg ccggatatcc acgttaatgg
     2401 cttccggctc ataacgaccg ggtgtcccca cctgactgaa aatatctgtc ggtacaggct
     2461 gatattcccc cagtttccgc cccagttttt cagctgtcca tgacggtccg aacgaactga
     2521 gtgcaacacc ttcccgcgct ctgtcccagc catccttcac caccagttcc ccttcctgca
     2581 tcgtgatata cagaccatca ctggccagcc gtttatgcaa agataaccag tcctgtgcag
     2641 gctcttcacg cagcccggca atcgacatct gagcaatata ttccctgaac gtctgctttt
     2701 tcccgcgtcg ccaggcatta cggcgctcac gtaccagcgc tgttttgcgc acaatacggt
     2761 tgccgggtgc atgcacaaag cagccattat ccggcgcaaa tccgtgctta agttcaagtt
     2821 cacggcatgc acgactgagt ttttcctggc tccaggaaag ccagttaata taaccagttt
     2881 caggatgtac acgattcacg gcaacatgca catgcagatt atccgtgtcc gtatgaacgg
     2941 cagccacgta ctgatgtttt gagagttcaa gagatttcag tgtgtgacgc acacagtcaa
     3001 aaagctgctc aggacgcggg ctttcatgcg caggccagga aagaatgtag tggaaaacgg
     3061 ggtctgtatc attacgggaa aaaacagctt tgtccgcctt gtactgcatc tcttcagcag
     3121 ctgtttccag tgaattgcag ttatggaaac aggttacgcc atagaaattt acccactgac
     3181 tgccgtcttt catcacatca atcagggaga caaatttttc gttacgcagg cgtgttgcat
     3241 aatcaacaag ccgtctgaaa cggttgcggt gagggacatc actcttgatt tcgctgtctt
     3301 ctgtgagctc attttcctgc acatcatccc gcacagacgt gtacgcaatc aggtcttcaa
     3361 aggaagacct gccgtcacgt cttttgtgcg gaataatggc attcatcagc agttatctca
     3421 gcgtcccatt aagtctcttc gcagagcatt gacatattca gtaatggcaa caagaacctc
     3481 tgcatactcc ttatcaccag tgcgttttcc ctccacaaac agatgcttct gaaggcggcc
     3541 aagacgcata atctcatcaa tcatacggtc gtcagtacgg cagttaacca ccttatccag
     3601 tgaagagctt cgtataaatg ttgaaaccgt ctgtccactt gactccgctt tttctcttac
     3661 gatttcagcc tcatcctccg tgaatctcgc cgtgaatttt acaattctct tcctgttctc
     3721 actgcccgtt cgggttttct tctcactcat ggtctgctcc ttaccggggt gtcggggcaa
     3781 agccctgacc aggtggggaa tgtctgagtg cgcgtgc
//
LOCUS       DAPHCV010000238         3790 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.243, whole genome shotgun sequence.
ACCESSION   DAPHCV010000238 DAPHCV010000000
VERSION     DAPHCV010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.243"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            120..2261
                     /locus_tag="RH033_004676"
     CDS             120..2261
                     /locus_tag="RH033_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HDV2993735.1"
                     /translation="MAMFTPSFSGLKGRALFSLLFAAPMIHATDSVTTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHATRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KKAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESVGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
     gene            complement(2369..3700)
                     /locus_tag="RH033_004677"
     CDS             complement(2369..3700)
                     /locus_tag="RH033_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2993736.1"
                     /translation="MKLTINEKIGFGAGDMAIAIVMMSLAMIITYFYTDVFGLKPVDL
                     GILLFSVRILDAVIDPVVGTMTDRTNTRWGKYRPWLLFMSIPFGISIWLMFTTPNTDY
                     SVKLLWAWATYVLLTLTYTLIAIPYVSLISVITDDPQERLSANGYRFVMTKIAMFAVT
                     IIVPLSAMYLGKNNVQLGYQIAMGAMGILATCLCLYCFFSVRERIYHPKPSLGMAAQF
                     RLLIKNDQWLILGAVIAIIMFGGIIRNSVAAYYAKYYLNGGDALISPFLTSGVIASVL
                     AMIACTWLTRLYDKIKIFRYTQLLAFVVGGAMYFVQPNSIVLAFTFYIVVTFLTDIQL
                     PIYWASIAESVDYGEMKTGQRVSGLAFGGILFFQKFGMGLAGGFIGIALAFMDYQPGV
                     EQTPQALWGICLLMTIIPAILNLITGLIMRFYLINNEYYEQIKVCLQPKNI"
BASE COUNT         1142 a          884 c          809 g          955 t
ORIGIN      
        1 gattgaatga ttctcaatta catatccgat ggcgaaataa caaacggata gtgcttagtg
       61 agcaataaga aacaattttc actcgcgaca atccgcgagt ttttgggata acagactaaa
      121 tggctatgtt cacaccttca ttctcaggac tcaaaggtcg ggcgctcttt tcactgcttt
      181 ttgcggcacc gatgattcat gcaacagact ctgtaacgac caaagatggc gaaacaatca
      241 ctgttacagc agatgcaaat accgcaactg aggcaaccga tggttatcaa cctctgagca
      301 cctccacggc gacattaacc gatatgccga tgctggatat cccgcaggtg gtcaatacgg
      361 ttagcgatca ggttctggaa aaccagaatg cgacaacgct ggatgaggcg ctttataacg
      421 tcagtaacgt ggtacagacc aatacattag gcgggactca ggatgctttt gtacgccgtg
      481 ggtttggcgc aaaccgggat ggctccatca tgaccaacgg tctgcgaacc gtacttcctc
      541 gcagtttcaa cgccgccaca gaacgtgtgg aagtgctaaa aggcccggcc tccacgctgt
      601 atggcattct cgatcctggc ggactgatta acgtcgtgac caagcgcccg gaaaaaacat
      661 tccatggttc ggtttcagcc acctcctcca gttttggtgg cggcactggg caacttgata
      721 tcacaggtcc cattgaaggc actcagctgg cgtatcgcct taccggggaa gtgcaggatg
      781 aagattactg gcgaaacttc ggtaaagagc gcagtacatt tattgccccg tcactcacct
      841 ggtttggtga taatgcaaca gtaaccatgc tctattccca tcgggactat aaaactccgt
      901 tcgatcgtgg aacgattttc gaccttacga cgaaacagcc cgtaaacgtt gatcgaaaaa
      961 tacgttttga cgaaccgttt aatattacag atggtcagtc cgatctggcg caactcaacg
     1021 cagaatatca tctcaatagc cagtggacag cgcgctttga ttacagctac agccaggata
     1081 aatacagcga taatcaggcg cgtgttaccg cgtatgatgc aacgacagga acactgacac
     1141 ggcgtgttga tgcaactcag ggatctaccc agcgtatgca tgctactcgt gcggatctgc
     1201 aagggaatgt tgatattgcc ggattctata atgagattct gggtggggtg tcatatgaat
     1261 attatgatct tctgcgtaca gatatgattc gctgtaaaaa agctaaagat ttcaatatct
     1321 acaaccctgt ttatggtaat accagcaaat gtacaacggt ttcggcgtcg gacagtgatc
     1381 agacgatcaa acaggagagc tactcagcgt atgcacagga cgcactctat ctgaccgata
     1441 actggattgc cgtcgccggg atccgctatc agtattacac gcagtatgcg ggtaaaggcc
     1501 gtccttttaa tgtcaatact gacagccgcg atgaacaatg gactcccaaa ctggggttgg
     1561 tctacaaact gacgccatcg gtatccttat ttgccaatta ttcgcaaaca tttatgccgc
     1621 agtcgtcaat tgccagctac attggcgatc ttccaccaga atcatctaat gcttacgaag
     1681 tcggggcaaa attcgagcta ttcgatggta tcaccgcaga tattgcgctg tttgatatcc
     1741 ataaacgtaa tgtgttgtat accgaaagtg ttggtgatga aaccattgcc aaaacggcag
     1801 gccgtgttcg ttcaagaggg gtagaagtcg accttgcggg agcattaact gaaaacatta
     1861 atatcattgc cagctacggc tataccgatg ctaaggttct ggaagatcct gattatgcag
     1921 gtaaaccatt gccgaatgtt cctcgtcata ccggttcgct attcctgacc tatgacattc
     1981 ataacatgcc tggcaataac acactgacgt ttggcggtgg tggacatggc gtaagccgtc
     2041 gttcggcaac caatggggct gactattatc tgccaggcta ttttgttgcc gatgccttcg
     2101 ccgcatacaa aatgaaattg cagtatccgg tcacactgca attaaacgtc aaaaacctgt
     2161 ttgataaaac gtattacacc tcttccatcg ccacaaataa tctggggaat cagattggcg
     2221 atccgcgtga agtgcaattc acggtgaaaa tggaattttg atcaaaaaag cccggtatgt
     2281 ttaccgggct gagactgatg acaaactcca aattgtctga tgcgctacgc ccatcagatc
     2341 taccatagaa attcaatata ttaaattttc atatattttt aggctgtaaa cacaccttaa
     2401 tctgttcgta atattcatta ttaatcagat aaaatctcat gatgagtccg gtaatcaggt
     2461 taagtatcgc gggaatgatc gtcattaaca ggcagatccc ccacaacgcc tgtggtgttt
     2521 gttctacgcc aggttgatag tccataaacg ctaaagcgat tccgataaac cctcccgcca
     2581 gtcccatacc aaacttttga aaaaagagaa tcccgccaaa tgccagtcca gaaacacgtt
     2641 gccccgtttt catttcacca taatccactg actcagcaat ggatgcccaa taaatgggaa
     2701 gctgaatatc agtcagaaag gtcaccacaa tataaaatgt aaaagctaac actatagaat
     2761 ttggttgcac gaaatacatc gcaccaccca ccacgaacgc cagtaattgg gtataacgaa
     2821 atatttttat tttgtcgtag agtcgagtaa gccaggtgca agcgatcata gccagaacag
     2881 aggcgatcac gcctgaagtt aaaaacggtg aaattaaagc atccccacca ttcaggtaat
     2941 attttgcata atatgcagcg acagagttac gaataatccc gccaaacata atgatcgcaa
     3001 ttacagcgcc caaaatgagc cattgatcat ttttgatcag taaccgaaac tgcgcggcca
     3061 taccgagact gggttttgga tggtagatgc gctctctcac gctaaaaaaa caatacaggc
     3121 ataaacacgt tgccagaatt cccattgccc ccattgctat ttgataaccc agttgtacgt
     3181 tattcttccc aagatacatt gcagataaag gaacaataat tgttacggcg aacatggcaa
     3241 tttttgtcat aacaaaacga taaccattgg cgctcaatcg ctcttgtgga tcatccgtga
     3301 taacgctgat taatgaaaca taaggaatag cgattaacgt ataggtcaag gtaagtaaca
     3361 cataggtagc ccaggcccat aaaagcttaa ctgaataatc ggtattaggt gtggtgaaca
     3421 tcaaccaaat gcttatccca aacggtattg acataaaaag caaccacggc cgatattttc
     3481 cccaacgtgt attcgttctg tctgtcattg ttcccacaac tggatcaatc acagcatcaa
     3541 ggatacgtac agaaaataac agaataccta agtcgacggg tttcagacca aatacatccg
     3601 tgtaaaaata agtgatgatc atggccaaag acatcattac tatcgcgata gccatatcac
     3661 cagcaccaaa acctatcttt tcatttattg taagtttcat acacagcact cattgattac
     3721 gtgaacttat attaaaaaca aatatatctg aactcattca gcacataact ttacgagtga
     3781 gcataaatgc
//
LOCUS       DAPHCV010000239         3786 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.244, whole genome shotgun sequence.
ACCESSION   DAPHCV010000239 DAPHCV010000000
VERSION     DAPHCV010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.244"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..2867)
                     /locus_tag="RH033_004678"
     CDS             complement(<1..2867)
                     /locus_tag="RH033_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV2993737.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDAGTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASAKATEAEKSAAAAESSKSAAATSAGAAKTSETNASASQQSA
                     ATSASTATTKASEAATSARDASASKEAAKSSETNAASSASSAASSATAAANSAKVAKT
                     SETNARSSETAAGQSASAAAGSKTAAALSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQASAAASSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESQAATPKAVKAAYELANGKYTAQDATTA
                     QKGIVQLSNATNSTSEMLAATPKSVKAAYDLANGKYTAQDATTAQKGIVQLSSATNSA
                     SETLAATPKAVKAANDNANGRVPSARKVNGKALSADITLTPKDIGTLNSTTMSFSGGA
                     GWFKLATVTMPQASSVVSITLIGGAGYNVGSPQQAGISELVLRAGNGNPKGITGALWQ
                     RTSAGFTNFAWVNTSGDTYDIYVAIGNYATGVNIQWDYTSNASVTIHTSPAYSANKPE
                     GLTDGTVYSLYTPSEQFYPPGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAVAYPSG
                     VIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTN
                     NTGAHTHSLSGSTNAAGNHSHRDGRRFNPSVFKDTYQYGYTSSGQNTWGVQGSVGMST
                     GWLANTSTDGNHSHSLSGTAASAGAHAHTVGIGAHTHSVAIG"
     gene            complement(2932..3531)
                     /locus_tag="RH033_004679"
     CDS             complement(2932..3531)
                     /locus_tag="RH033_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HDV2993738.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDTLGMVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     WAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            complement(3601..>3786)
                     /locus_tag="RH033_004680"
     CDS             complement(3601..>3786)
                     /locus_tag="RH033_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HDV2993739.1"
                     /translation="ASRETKGAIPGSYSAVIDMPSGRGSVTLEFKVFHKGNQWAGNIT
                     DCTVIVTKKAASGISIR"
BASE COUNT          769 a         1077 c          962 g          978 t
ORIGIN      
        1 ccaatcgcaa cggagtgcgt atgagcacca ataccgacag tatgcgcgtg tgcacctgca
       61 gatgctgctg tgccggacag tgagtggcta tgattaccat ctgtactggt attcgctaac
      121 caccccgtag acatacctac tgagccttgt acaccccagg tattttgacc tgagcttgta
      181 taaccatatt gataagtatc tttaaaaaca ctggggttaa atcgacggcc atctctatgg
      241 ctgtgattac cagccgcatt cgtgctgcca cttaaactat gggtatgcgc accagtgtta
      301 ttcgtggatt tagtgccgta atcaaacgac gatgtggttt tcgtccccaa atccgtactg
      361 gatgcgctgg cgctgtgggt atgcgattta atgccgtcct gttcctgaga caatacggcc
      421 cgaccactgg caggtttgcc cttaatcgtc cagccacgca tatcagggat cacgcctgac
      481 ggataagcga ctgcaagttt cgggtaggca gatttgtcaa aagtctgccc ctgcatcagg
      541 gcataaccag acggaacggt atccgatggc cacgggattg gtgcgccagg cggataaaac
      601 tgctctgatg gcgtatagag tgaataaact gtaccgtccg ttaacccttc cggcttatta
      661 gcagaatatg ctggtgacgt atgaatcgtt acgctggcat tactggtata atcccattga
      721 atatttacac cagtcgcata atttccgatt gcaacgtaaa tatcgtaagt atcaccagat
      781 gtattgaccc aggcaaaatt tgtaaaccct gccgatgtgc gctgccataa agcaccagta
      841 atccccttcg gattaccatt acctgcacgc aaaacaagtt cagatatacc tgcctgttga
      901 ggtgaaccga cgttatatcc agccccacca atcaacgtaa ttgaaacaac agaactcgcc
      961 tgcggcatgg ttaccgttgc taatttgaac caaccagcac caccgctgaa tgacatagtt
     1021 gttgagttaa gcgtaccaat atctttcggt gtcagtgtta tatccgctga aagcgcctta
     1081 ccattcacct tacgggcaga aggtacccga ccattcgcat tatcattagc tgctttcact
     1141 gctttcggtg tcgcggcaag cgtttcagat gcgctgttgg ttgcactact gagctggaca
     1201 attccttttt gtgctgtcgt agcgtcctga gcagtatatt tcccgttagc aaggtcatag
     1261 gctgccttta ccgactttgg cgttgccgcc agcatttcag atgtgctgtt ggtcgcgttg
     1321 ctaagctgaa ctatcccttt ctgtgctgtc gttgcatcct gtgcggtgta tttcccgtta
     1381 gccagctcat acgcggcctt aacggctttt ggcgttgccg cctgtgactc ggaagtgctg
     1441 ttggtcgcgc tgcttagctg gactatcccc tttttcgtcg tgctcgcatc ctcaagcgcc
     1501 acggcggatg caatatcctc tgcccgtttt gccgctgtct cggcgcgcgt tgccgcggat
     1561 tccgccgtac ttttgctctg agctgccgcc gtcgcactgc cagctgcctc tgtcgccttc
     1621 gtggatgccg tcgtggcgct gccctttgct gctgacgcct gtctggtcgc ctcatctttt
     1681 gaagcagacg cagatgatgc cgatgacgcc gccgaactgg ctgacgatgc ggcagccgtt
     1741 tttgaggatt ctgcgctggt ttccgacgct ttcgcgttcg tttcggatgt cttcgctgcg
     1801 gaagcagaac tcgctgctgc gctggcctgt tcagtggctt cgccagcctt cgttgtggct
     1861 gttgaagcag acgatgcagc actttctgcc gattttccgg cggcggtggc actggctgag
     1921 gcctgcccgg cacttgttga cgcggcactg gcagataatg cagccgctgt ttttgagcct
     1981 gccgcagctg aggcgctctg tcccgctgcc gtttcagaag acctggcgtt cgtctcggac
     2041 gtttttgcca ccttcgcaga atttgctgcc gccgttgccg aggaagcggc actactggcg
     2101 ctcgaggctg cgttcgtttc tgatgatttc gctgcctctt ttgaggccga cgcatcccgt
     2161 gctgaagtgg ctgcttctga cgctttcgtg gtcgcggtgg atgcagaagt ggcggctgat
     2221 tgttgtgacg ctgaagcatt cgtttcagac gttttcgcgg caccggcact ggtagctgcc
     2281 gcgctttttg aggactctgc agcggcagca cttttttccg cttcagtggc ctttgctgat
     2341 gccgttcctg cgctggaaga cgctgactga gccgacgacg cggcctgtcc ggctgatgtg
     2401 ctggctgcgc gtgctgagtc tgcagcatca gtcgcatggg ttgccgcctc acgggcagat
     2461 gtgccggcat cgctggctga cttctttgcg gctgccgtgt tctgtgccac tgcggacgcg
     2521 ttacgcgcca cctcttccac catcagctca aaacggcgca gtgcctccgg acgggcatca
     2581 tcctccgtca tggcaccgag aaaatcattc agcgtaccgg gttgagaatc ttcatacacg
     2641 gtgatggtcc cggcatgtga cggcgggaat ccctccacca acagaataac gctgtactga
     2701 ccgtactcaa cgtccatact gtaacgcccg gcttcatccg gattttcaga tgccagcgtg
     2761 ttcaccacca ccgtggtgct gttacgtttt gctttcagct ggattgtgca gttctgtacc
     2821 ggttttcctg tgccgtcttt cagtacacct gaaatcttta ctgccatatt caccccacaa
     2881 aaaagcccgc ctgaaacggc gggctgtcat aacactgtgt tacctggcta atcagaactt
     2941 ataaccgaca cccacgatga aaccatcagt gcgccagtca ccactgccgg aaccttcata
     3001 agcaatatca atggccacgg attcggtcgg gttaaactgc acgccagccc accacgccag
     3061 agacgtgttg ctgtggcgac cgtcatcact tccggtcagc acatcatgcg ttttcccctt
     3121 gttgtcagtt acgcggagat aatccccgga aaaagtcgac acacggctgt aagccacgcc
     3181 cgccatcgca tacgcactga accattcatt cacgcgcaca gagggccccg ccatcacgct
     3241 gaaccagcgg ttacgcacag aatcttcatg ccagcgggta tcgctgtaac gggtcagctg
     3301 gcgattcttg tctcctgcat agctgaatga cgtcaccatc cccagcgtat ccgtaaactc
     3361 ataacggtat ttcacgttaa tcccgttcag atcatcgctg ccgggaacgt tggtcgaggc
     3421 atgaagatac cccgcgctca gcgtggactg atgttcagat gcccatgcag gcgcaccgga
     3481 tacggccaga caaatggctg cggacaaaat tgctgcacaa actttacgca taattacctc
     3541 tcgcttttct gcaataaaaa aggcaccatt tctggtgccc ttatatgggt tataacaatt
     3601 tcaacgaata ctgatgccgg aagcggcttt tttggtcaca atcaccgtac agtcggtgat
     3661 attacctgcc cactgattgc ctttatggaa aaccttaaac tccagagtga cgcttcccct
     3721 gccactcggc atatcaataa ccgcactgta gctaccggga atggcccctt tagtttctct
     3781 ggatgc
//
LOCUS       DAPHCV010000240         3774 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.245, whole genome shotgun sequence.
ACCESSION   DAPHCV010000240 DAPHCV010000000
VERSION     DAPHCV010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.245"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..260
                     /locus_tag="RH033_004681"
     CDS             <1..260
                     /locus_tag="RH033_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDV2993740.1"
                     /translation="YQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLS
                     KRERYTDKWQRRTHSLWRHRAWRASAFTGIYNDLVAASTFV"
     gene            197..799
                     /locus_tag="RH033_004682"
     CDS             197..799
                     /locus_tag="RH033_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDV2993741.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDTGETELSARLNEPLANGAVIHIVPRLAGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKPKTPSTQTTDNGKQNTYFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            860..>3774
                     /locus_tag="RH033_004683"
     CDS             860..>3774
                     /locus_tag="RH033_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HDV2993742.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDLSGTVNGVTPPAVQHL
                     TAEVSADSGEYQVLARWDTPKVVKGVSFMLRLTVTADDGSERLVSTARTTETTYRFRQ
                     LALGNYRLTVRAVNAWGQQGDPASVLFRIAAPATPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRITDIRQVETTARYLGTALYWIAASINIKPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITNVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMVNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     "
BASE COUNT          862 a          950 c         1195 g          767 t
ORIGIN      
        1 tgtatcaggt gccgttgtca tcagcacaac cgggcgatgt gctgctgtgc tgttttggtt
       61 catcggtgcc gaatcatgcc gccatttact gtggtgatgg cgagctgctg caccatattc
      121 ctgaacaact gagcaaacga gagaggtata ccgacaaatg gcagcgacgc acacactccc
      181 tctggcgtca ccgggcatgg cgcgcatctg cctttacggg gatttacaac gatttggtcg
      241 ccgcatcgac cttcgtgtga aaacgggggc tgaagccatc cgggcgctgg ccacacagct
      301 cccggcgttt cgtcagaaac tgagcgacgg ctggtatcag gtacggattg ccgggcgtga
      361 tacaggtgaa acggaattat cagcccgtct taatgaaccg ctggcaaatg gtgctgtgat
      421 ccatatcgtg ccgcgtctgg caggagccaa aagtggcggt gtgtttcagg ctgtgctggg
      481 ggcggctgtt atggcggttg ctatatggat gccgggggta ggaattatgg cgagtaatct
      541 gctgttttct ctcggtgcca gtatgacgct tggcggtgtt gcacagatgc tggcccctaa
      601 acccaaaacc cccagcacac agacaacgga taacggcaaa cagaacacct atttttcttc
      661 actggataat atggttgccc agggcaatgt tctgcccgtt ctgtacggtg aaatgcgcgt
      721 gggatcacgt gtggtatctc aggagatcag cacagcagat gaaggtgatg gtggtcaggt
      781 tgtggtgatt ggtcgctgat gaaaaacgtt ttatgtgaaa ccgcctccgg gcggttttat
      841 cgtttatgga gcatgacgaa tgggtaaagg cagcagtaag gggcataccc cgcgcgaagc
      901 gaaggacaac ctgaagtcca cgcagctgct gagtgtgatc gatgccatca gcgaagggcc
      961 ggttgaaggt ccggtggatg gattaaaaag cgtgctgctg aacagtacgc cggtgctgga
     1021 cagtgagggg aataccaata tatccggcgt cacggtggtg ttccgggccg gtgagcagga
     1081 gcagtcaccg ccggagggat ttgaatcctc cggctccgag acggtgctgg gtacggaagt
     1141 gaaatatgac acgccgatca cccggaccat cacgtcggca aacatcgacc gtctgcgctt
     1201 taccttcggt gtacaggcac tggtggaaac cacctcaaag ggtgacagga atccgtcgga
     1261 agtccgcctg ctggttcaga tacagcgtaa cggtggctgg gtgacggaaa aagacatcac
     1321 cattaagggc aaaaccacct cgcagtatct ggcctcggtg gtggtggata acctgccgcc
     1381 gcgcccgttc agtatacgga tgcgcaggat gacgccggac agcaccacag accagctgca
     1441 gaacaaaacg ctctggtcgt catacaccga aatcatcgat gtgaaacagt gctacccgaa
     1501 cacggcactg gtcggtgtgc aggtggactc ggagcagttc ggcagccagc aggtgagccg
     1561 taattatcat ctgcgcgggc gtattctgca ggtgccgtcg aattataacc cgcagacgcg
     1621 gcaatacagc ggtatctggg acggaacgtt taagccagca tacagcaata acatggcctg
     1681 gtgtctgtgg gatatgctga cccacccacg ctacggcatg gggaaacgtc ttggtgcggc
     1741 ggatgtggac aaatgggcgc tgtatgtcat cggccagtac tgcgaccagt cagtgccgga
     1801 cggctttggc ggcacggagc cgcgcatcac ctgtaatgcg tacctgacca cacagcgcaa
     1861 ggcgtgggat gtgctcagtg atttctgctc ggcgatgcgc tgtatgccgg tatggaacgg
     1921 gcagacgctg acgttcgtgc aggaccgacc gtcggataag gtgtggacct ataaccgcag
     1981 taatgtggtg atgccggatg atggcgcgcc gttccgctac agcttcagcg ccctcaagga
     2041 ccgccataat gccgttgagg tgaactggat tgacccggat aacggctggg agacggcgac
     2101 agagcttgtg gaggacacgc aggccattgc ccgttatggt cgtaacgtca cgaagatgga
     2161 tgcctttggc tgtaccagcc gggggcaggc acaccgagcc gggctgtggc tgattaaaac
     2221 ggagctgctg gaaacgcaga ccgtggactt cagcgtgggt gctgaagggc ttcgccatgt
     2281 accgggcgat gtcattgaaa tctgcgatga tgactatgcc ggtatcagca ccggcgggcg
     2341 cgtgctggcg gtgaacagcc agacccggac gctgacgctc gaccgtgaaa tcacgctgcc
     2401 atcttccggc accacgctga taagcctggt tgacggaagt ggcaatccgg tcagcgtgga
     2461 ggtccagtcc gtcaccgacg gcgtgaaggt aaaagtgagc cgtgttcctg acggcgttgc
     2521 cggatacagc gtatgggggc tgaagttgcc gacgttgcgc cagcgcctgt tccgctgtgt
     2581 gagtatccgt gagaacgatg acggcacgta tgccatcact gccgtgcagc atgtaccgga
     2641 aaaagaggcc atcgtggata acggggcgca ctttgacggc gatttgagcg gcacggtgaa
     2701 tggtgtcacg ccgccagcgg tacagcacct gacagccgaa gtctccgcag acagcgggga
     2761 atatcaggtg ctggcgcgct gggacacacc gaaggtggtg aagggggtga gctttatgct
     2821 tcgcctgacc gtgacagcgg acgatggcag tgagcggctg gtcagcacag cccggacgac
     2881 ggaaaccaca taccgcttca ggcagctggc gctgggaaac tacaggctga cagtccgggc
     2941 ggtaaatgcg tggggacagc agggcgatcc ggcgtcggta ttgttccgga ttgccgcacc
     3001 ggcgacgccg tcgcggattg aactgacgcc gggctatttt cagataaccg caacgccgca
     3061 tcttgccgtt tatgacccga cggtacagtt tgagttctgg ttctcggaaa agcggattac
     3121 cgatatcagg caggttgaaa ctacagcccg ctatcttggc acggcgctgt actggatagc
     3181 tgccagtatc aatatcaagc cgggtcatga ttattatttt tacgttcgca gtgtgaacac
     3241 tgttggcaaa tcggcattcg tggaggctgt cggtcgggcg agcgatgatg cggaaggtta
     3301 cctggatttt ttcaaaggag aaatcgggaa aacacatctg gcccaggagc tgtggacgca
     3361 gattgataac ggtcagcttg cgccggacct ggctgaaatc aggacgtcca ttacgaatgt
     3421 cagcaatgaa atcacgcaga ccgtcaataa aaaactggaa gaccagagtg cggcaatcca
     3481 gcagatacag aaagttcagg ttgatacaaa taataacctg aacagcatgt gggctgtgaa
     3541 gctgcagcag atgcaggacg gacgccttta tatcgcgggt attggtgccg gtattgagaa
     3601 cacccctgac ggcatgcaga gtcaggtgct gctggcggcg gacaggattg cgatggttaa
     3661 tcctgcgaat ggcaacacaa aaccgatgtt tgttggtcag ggcgatcaga tattcatgaa
     3721 cgacgtgttc ctgaaacgcc tgacggcccc caccattacc agcggtggaa atcc
//
LOCUS       DAPHCV010000241         3742 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.246, whole genome shotgun sequence.
ACCESSION   DAPHCV010000241 DAPHCV010000000
VERSION     DAPHCV010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.246"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(269..556)
                     /locus_tag="RH033_004684"
     CDS             complement(269..556)
                     /locus_tag="RH033_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HDV2993743.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLFIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(631..774)
                     /gene="mgrB"
                     /locus_tag="RH033_004685"
     CDS             complement(631..774)
                     /gene="mgrB"
                     /locus_tag="RH033_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HDV2993744.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            933..1172
                     /locus_tag="RH033_004686"
     CDS             933..1172
                     /locus_tag="RH033_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993745.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1316..2107)
                     /gene="kdgR"
                     /locus_tag="RH033_004687"
     CDS             complement(1316..2107)
                     /gene="kdgR"
                     /locus_tag="RH033_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HDV2993746.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2284..3657
                     /locus_tag="RH033_004688"
     CDS             2284..3657
                     /locus_tag="RH033_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2993747.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
BASE COUNT          930 a          932 c          821 g         1059 t
ORIGIN      
        1 aaattgatga atgcgcaaaa aaaatcaaga gagaaacgtt tcgctaataa ataataccac
       61 gcttaatcac aaatccgatg taaacatcct aataaattaa tgggctaaat atctttttct
      121 gatatcccgt tacgcaaata aataatatga taattatttg aagtcagacc agagttttaa
      181 tttgaagcag ttgcaccatg acagtgcgta taatcaggat gaattgagta acccttcaac
      241 gacggggtta ctcaagattg aggaaggatt atcgttcggc gaccagacga ataatatctt
      301 cgtcttcaac cgatttttga ctctcaacgg tctttttatc tgctttctca ggttcgtttg
      361 cttcgcacaa acgacgtaac agagcatttt gacgtttttg ctgatccagc aacgcctcaa
      421 gcagttcaat ctgttcgtta gtccgtgaac tggcacgatt gataaaaaac cacaagataa
      481 gcccgataaa aagaaccacc accgatacaa ccaaagacgc aatgttcatc acgcctgaat
      541 ttacaacttc gttcatttca ccacctctga gtagaggcgc tattctacca ctgctggaga
      601 ggaagaaaat ctagtgctga aaaaatgata tcaccacggg ataaactggt taatggcaca
      661 aattccgctg aaaaattgta catcctgatc gcacatcatg ttgaatacct gcgcccaaag
      721 cagcaagcaa gccaacacca cgacaaccag aacgacccat cgaaactttt tcactccaat
      781 ctccgtttca cctaccttat gtcattaaac tagcatgcgt tagttaaaca gcgcctaact
      841 atgtcgatat tcgtgctttt tcagaatgag tcgcttgctt cagcgcatat tgccgctacg
      901 attaagcgaa cataaaaaga gaagaggttg taatgcgatt catcattcgc acagttatgc
      961 tgattgcact ggtatggata ggattattac tcagcggata tggcgttttg attggaagca
     1021 aagagaatgc cgcgggatta ggtttgcaat gtacctatct gaccgccaga ggcaccagca
     1081 cggtacaata cctgcacact aaaagtggtt ttctggggat aacagattgc ccgttgttac
     1141 gtaaaagcaa tattgtggtc gataatggtt aagggtaata aataaccaga ttctcactca
     1201 tgagtaaaat attcttatcg caagtgcgtt taatataacg ccaccttcaa ctttattttt
     1261 ctcatgctaa ataaagaacc tgtctgtttg atattcagac aggttcttac tctcatcaga
     1321 acggatagtc gtgataaccc atttgggcag aaattttgcg cgccgcggta tgcaacattg
     1381 cgacatattc ctgtaaacgc tcttcagaga aacgcaacgt cgggaaggag atgctaagac
     1441 cggcaatgac cacgccaaag cgatcaaata ccggaaccgc aatgcatcgc agcccttctt
     1501 cctgctcttc gttatcttcg ccatacccct gctcgcgcac ctggtccaga acgggtaata
     1561 acgcttctgt actggtgatg gtccgctcgg tactgcgttt atactccacg ccctcaagaa
     1621 tttgcttcac ttcatcgcga tcgcgccatg ccagcagtac cttaccaatc gcggtgctgt
     1681 acagcggatt acgacgccca atccgtgaat acatgcgcaa attgtacata gagtcaattt
     1741 tgtgaatgta aacaatactg tcttcgtcca gtgcgccgag gtggatagtt tctttggtca
     1801 ggcgggagat ctcacgcatc tggatatctg cgctacgaat taaatcgacg ttttgtaacg
     1861 cgcgagcgcc cagttcaaac aatttcaggg tcagcgaata tttctccgac tccccttcct
     1921 gcgccacata acctaaggtt ttcatggtct gtaaaaagcg ataaacggtg ctttttgaca
     1981 tcatgacgcg ctgcgacaac tcggttatcc ctatttcgcg ctcttcaccc agcgcctgca
     2041 aaatgccaaa aacttttagc acggaagata cagaatcagg ctgtttatcc agatctgcgt
     2101 tagccattta tcacctcatt gcgagtgttt tataaaaatc agaactgttt tttattataa
     2161 tttcgcacca gggtggtcgc aatccatctt ttgccggtta gttacaattc tgcgacatcc
     2221 accgtgaata tcagtgctag aatcataccc ctgttgatta ttcaccaaag atataaaatt
     2281 cctatgccaa aagttcaggc cgacggcctg ccattgcccc agcgatacgg tgcgatatta
     2341 accattgtga ttggtatttc gatggctgtc cttgacggcg caatcgccaa cgtcgccctg
     2401 ccaacaatcg ccacggacct tcatgccacg ccagccagtt ccatctgggt agtgaacgcc
     2461 tatcaaatcg ccattgtcat ctccctgctc tcattttcgt ttctgggcga tatgtttggc
     2521 tatcgacgta tttataaatg cggtctggtc gtttttctgt tgtcttcact gttctgcgcc
     2581 ctttctgatt cgctgcaaat gctcaccctt gcgcgtgtca tacaaggttt cggcggtgca
     2641 gcgttgatga gcgttaatac cgcacttatc cgccttatct atccacaacg ttttctgggt
     2701 agagggatgg gcataaactc gtttattgtt gccgtctctt ctgctgccgg gccgacaatt
     2761 gctgcagcaa tcctctccat cgcatcctgg aaatggttat ttttaatcaa cgtaccgtta
     2821 ggtattatcg ccctgcttct ggcgatgcgt tttctgccac ccaatggttc tcgcgccagt
     2881 aaaccccgtt tcgacctgcc cagcgccgtg atgaacgcgt taaccttcgg cctgcttatc
     2941 actgcgttga gtggtttcgc tcaggggcaa tcgctgacgt taattgctgc ggaactggtg
     3001 gtaatggttg tcgttggtat tttctttatt cgccgccagc tttctcttcc cgtaccgctg
     3061 ctaccggttg atttactgcg tatcccgctg ttttcacttt ctatttgcac atctgtttgc
     3121 tctttctgcg cacaaatgct ggcaatggtt tccctgccct tttacctgca aaccgtgctc
     3181 gggcgtagtg aagtcgaaac aggtttactt ctgacaccgt ggccgttagc aacaatggtg
     3241 atggctccgc tggcaggcta tttgattgaa cgcgtacatg caggattgct gggtgcttta
     3301 gggttgttca tcatggctgc ggggcttttt tccctggttc tgctgcccgc gtcacctgcg
     3361 gatatcaata ttatctggcc gatgatctta tgtggcgctg gatttggctt attccagtca
     3421 cccaataacc acaccattat tacctccgct ccgcgcgaac gtagcggtgg ggccagtggc
     3481 atgttaggga cggctcgtct attgggtcag agtagtggcg cggcgctggt ggcgctgatg
     3541 ctaaatcagt ttggtgataa tggtacgcac gtctcgctga tggctgcggc tattctggca
     3601 gtgattgctg cctgtgtcag tggtttacgt atcactcagc cacgagccag ggcataataa
     3661 atatcctccg gcatagccgg aggtttttca aatgcgccta taaggctctc ttaccagccg
     3721 cgccctaaca ggcgcacacg at
//
LOCUS       DAPHCV010000242         3720 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.247, whole genome shotgun sequence.
ACCESSION   DAPHCV010000242 DAPHCV010000000
VERSION     DAPHCV010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.247"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            98..2762
                     /locus_tag="RH033_004689"
                     /pseudo
     CDS             98..2762
                     /locus_tag="RH033_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768542.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(2820..3155)
                     /locus_tag="RH033_004690"
     CDS             complement(2820..3155)
                     /locus_tag="RH033_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HDV2993748.1"
                     /translation="MMMKKFALLAGLFFFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(3159..3503)
                     /locus_tag="RH033_004691"
     CDS             complement(3159..3503)
                     /locus_tag="RH033_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HDV2993749.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            complement(3505..3678)
                     /locus_tag="RH033_004692"
     CDS             complement(3505..3678)
                     /locus_tag="RH033_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993750.1"
                     /translation="MSYLSFEIILIPVKNIIPIITVTLILDYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
BASE COUNT         1093 a          754 c          858 g         1015 t
ORIGIN      
        1 gcttttgttt ttgtctttac cctccgtttt ggtaatctga actcataggt tatttgacca
       61 ggaatgtttg ttaatccaat gaatggatgg ttaatctatg aaactgaaaa aactccccgg
      121 gtttagttta ggacttattg ctttggcggt gggtaatgca tatgcaacac aattgttgga
      181 tgattatagt atactttcct atataactga tgaagaatcg ccgattgaaa tcaaagataa
      241 taattcgata agtaatggag agtatctaac cactaaagac gaaagccatg ctgtgaaagt
      301 ggatgacggt gtaactggat atataaataa tgccagtgtg atgactagtg gtgatggatc
      361 ttatggtatt tctgttgata gtcataacaa agtattatat ataagtgata gcgatattaa
      421 gacctctgga agcgtatctg acaaaggaaa taaccaagat gttaatggag ggataacagc
      481 cagcgcagta gtcagtgaat ttggtggcac catcgttatg aatggtgata attcagtcga
      541 gacgaggggg gcatattcag cgggactttt aagtcaggtt aatgattctg ggattgtgga
      601 aaataacacc cgtcttgaaa ccacagataa aacgaacatt gttacctatg gggaaaatgc
      661 agtaggtgtt cttgcatgtt caagtcctgg agagtctcga acatgtgtcg atgctgtaga
      721 tgatgaagtt agtgattcta atagttacga agttattagc cgtgctgatt taaaaatgaa
      781 tggtggttcc ataacaacta atggcactaa tagctatggt gcttatgcta atggggaaaa
      841 agcatatatt aatttagatt atgtggcact tgaaaccggg tagcatggaa gttatgcagt
      901 tgctattcga caaggtaaca ttgatataaa aaatagttct attacaacaa aaggcactaa
      961 agcccccatt gcaaaaatat acaatggtgg agagttattt tttttccaat gtcaccgcgg
     1021 tatcagaaca agataaagga atatcaattg atgcatcaaa tatcgattct caagccaaaa
     1081 tagcactatc aagtgtcgaa ctttcaagtg ctttggatag tattgatgtt aacaaaacta
     1141 caacggatgt aagcatcctt aatcgaagta ttatcacgcc tggtaataat attctggtta
     1201 ataatactgg aggtggctta aacataattt cgtccgactc tactctaaat ggagcgacta
     1261 aactcgttag cggcacaacc acactgaagc tttcagaaaa cacaatctgg aatatgaaag
     1321 atgactccgt tgttacccat ctgactaatt cagacagtat tatcaatctt tcgtatgatg
     1381 atggtcaaac atttacccaa ggaaaaacgt taaccgtaaa aggtaattat gtcggtaata
     1441 atggtcagct taatatccgc accgtattag gtgatgataa atcggctacg gacagactta
     1501 ttgttgaggg taatacttcg gggtcaacta ccgtctatgt gaaaaatgct ggaggaagcg
     1561 gcgcggccac gctaaatggg atcgaactca taactgtgaa tggcgatgaa tctccagcag
     1621 atgccttcag acaaggtgat gcaaggattg cagctggagc tttcgaatat caactaaaac
     1681 aacaaggcaa aaactggtat ttgacaagtt atcagtcatt gaatgaggag gataacagct
     1741 cagagggaga ttcagaatca atagaaaatc ctacccccgt cctgcgcccc gaagctggaa
     1801 gttacgtcgc caacctggca gccgctaaca ctctttttgt tatgcgtctg aacgatcgtg
     1861 caggtgaaac gcgttacatc gatcctgtaa cttaacagga gcgttcaagc cggctttggc
     1921 tacgtcaaat tggcgggcat aatgcctggc gtgacagcaa cggacagttg agaacgacct
     1981 cgcatcgcta cgtctcgcag ttaggggcgg atctgttaac cggtggtttt accgacagtg
     2041 acagttggcg tttgggagtg ttggctggtt atgcccgcga ctacaactca actcattcca
     2101 gcgtgtcgga ttatcgttcg aaagggagtg tcagaggcta tagcgcaggg ctgtatgcca
     2161 cctggtttgc cgatgacatc agtaaaaaag gcgcatacat tgacgcctgg gcgcaatata
     2221 gctggtttaa aaactcggtg aaaggggatg aattagccta tgaatcctat agtgcgaaag
     2281 gcgcaaccgt ctcgctggaa gcgggttacg gctttgccct gaataaatcc tttggtctgg
     2341 aagcggcgaa atatacgtgg atcttccagc cacaggcaca ggctatctgg atgggcgtcg
     2401 atcatcatgc gcacaccgaa gccaatggct cacgtattga gaatgacgca aataacaaca
     2461 tccagacccg acttggtttc cgcaccttta ttcgtactca ggagaaaagc agcggtctgc
     2521 atggtgacga ctttgaacct tttgttgaaa tgaactggat ccataacagt aaagattttg
     2581 ctgtctcaat gaacggtgtg aaagttgaac aagacggggc gcgtaatcag ggggaaatta
     2641 aacttggcgt aaatggcaat ctgaatccgg cggccagcgt ctggggtaat gtgggcgtgc
     2701 agctgggtga taatggctac aatgacacca cagtgatggt gggcctgaaa tataagttct
     2761 gatcccgcca ttagctaaaa aaccgcgtcg atttcatcga cgcggtaaca tatgaaatat
     2821 tattttttgc cgatagcacg catggtgtca tcaattgccg tgatcaacag catttgcggg
     2881 tctttagcgc aaacctgatt cagtttttct accactttgg cgctcagttc cggagattgc
     2941 tcaattttta aatcacggct ggcaacgctg gcattaccca ttaccgcaac aatttctgca
     3001 acctgtgcgc tgtcagtttt tgccatttcg ttggcttctg cgcaagtaat ataggtttct
     3061 gacggcaaac cgtttttaat attgtagtcc tgcgcccagg tcattggtgc gaaaaaaaac
     3121 agacccgcca gtaaagcaaa ttttttcatc atcattcctt atttcatttt acccagaatt
     3181 gcaccacccg taccgccaat cacggcacct ttgatcgccc cttcgaggcc attgccggtc
     3241 agaacgccag tgacagcacc aacggcggca cccacttttg cacctttacg cgcattttta
     3301 ccgtcgcggc ctttttctgt tactgcacca acaccagcgc caacagctgc acctttcagt
     3361 acgccattaa caccattgcc agtaagtaaa ccaacgcctg cgcctagcaa tgcacctttc
     3421 gtggtgcggt tcatatccgc catcgctggc gtggagcaga acaatgctga gataagcccg
     3481 aaggcaagta tttttttctt caacttagat gtccggtatt aagtaagttg cacacacaat
     3541 aatttcgtct tcaattaaga tctgcttaac taaagaacgc tcgctattat tcagataatc
     3601 caaaatgagc gtgactgtga tgataggaat tatgtttttt acgggaataa gaataatctc
     3661 aaatgataaa taactcattg attgacaata tttttattca agaagtgtca ttgagtgtta
//
LOCUS       DAPHCV010000243         3641 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.248, whole genome shotgun sequence.
ACCESSION   DAPHCV010000243 DAPHCV010000000
VERSION     DAPHCV010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.248"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..2247
                     /gene="faeD"
                     /locus_tag="RH033_004693"
     CDS             <1..2247
                     /gene="faeD"
                     /locus_tag="RH033_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbrial usher FaeD"
                     /protein_id="HDV2993751.1"
                     /translation="GRYLVDLSLNGKEAGKQILDVTPQDSNELCLTEVWLTKAGIYVS
                     ADYFREGYDATRQCYVLTKAPSVKVDFDVSTQSLALSIPQKGLVKMPENVDWDYGTSA
                     FRVNYNANANTGRNNTSAFGSADLKANIGRWVVSSSATASGGDSGDNSATINMFTATR
                     AIRALSADLAVGKTSTGDSLLGSTGTYGVSLSRNNSMKPGNLGYTPVFSGIANGPSRV
                     TLTQNGRLLHSEMVPTGPFSITDVPLYTSGDVTMKITGEDGREQVQNFPLSVMAGQLS
                     PGQHEFSVAAGLPDDDSDLKGGVFAASYGYGLDGLTLRTGGVFNQDWQGVSAGAVLGL
                     SYLGAVSADGAYATAKYRDGSRSGNKVQLSWSKQLEMTNTGLRVSWSRQSEEYEDMSS
                     FDPTELWSQSNHGRRTKDEWNAGVSQPVGGLFSLSVSGWQRSYYPASMTGSYRYSDDN
                     GKDTGITGTLSTQIKGVSLNLGWSGSRNTRGENNWSASASVSVPFTLFDRRYSSSTSV
                     STSKDGGTGFSTGVSGSLNDRFSYGLGGGRDSGGGVSSYLNASYSGDRAYLNGALNHS
                     QSGGTSGSVSVSGSVLAVPAAKDIMFSRTTGDTVAVVNVKDTPGVKVTSGDGQTDSDG
                     NLVVPLNSYDWNTVTIDAGTLPLSTELTNTSQKVVPTDKAVVWMPFDALKVKRYLLQV
                     KQRDGEFVSGGTWARDSKNTPLGFVANNGVLMINTVDAPGDITLGQCRIPAAKLQDTE
                     KLQEITCE"
     gene            2240..3031
                     /gene="faeE"
                     /locus_tag="RH033_004694"
     CDS             2240..3031
                     /gene="faeE"
                     /locus_tag="RH033_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000044489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbrial chaperone FaeE"
                     /protein_id="HDV2993752.1"
                     /translation="MSKRNAVTTFFTNRVTKALGMTLALMMTCQSAMASLAADQTRYI
                     FRGDKDALTITVTNNDKERTFGGQAWVDNIVEKDTRPTFVVTPSFFKVKPNGQQTLRI
                     IMASDHLPKDKESVYWLNLQDIPPALEGSGIAVALRTKLKLFYRPKALIEGRKGAEEG
                     ISLQSRPDGRTMLVNTTPYIFAIGSLLDGNGKRIATDNETAQKLLMFMPGDEVQVKGN
                     VVKVDSLNDYGELQTWTINQKKTPTSSGQKASDSLVNPSDKADKK"
     gene            3066..3557
                     /gene="faeF"
                     /locus_tag="RH033_004695"
     CDS             3066..3557
                     /gene="faeF"
                     /locus_tag="RH033_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbria minor subunit FaeF"
                     /protein_id="HDV2993753.1"
                     /translation="MKKTMMAVALALSALSIQSAQAAEYREKTQYLGVVNGQVVGNSV
                     MKVTRTPTDPVLYRSGDTTPLPDNLTVRNAESREASGGLAYITVKQVLPDNGEARITL
                     KTALMVDGKKVALSARQQGEDVVISVPEAKKQVELRTDAPAELEVPVSYRGNLQIALQ
                     VED"
BASE COUNT          870 a          865 c         1194 g          712 t
ORIGIN      
        1 gggcgttatc tggttgacct gtccctgaac gggaaggagg ccgggaaaca gatactggat
       61 gtgacaccac aggacagtaa tgaactgtgt ctgacagagg tatggctgac gaaggccggg
      121 atttacgtca gtgcagatta ctttcgtgag ggatatgacg ccacacgaca gtgctatgtg
      181 ctgacaaaag ccccgtcagt gaaggtggat tttgatgttt ccacccagag tctggcgctg
      241 tccattcccc agaaggggct ggtgaagatg ccggagaatg tggactggga ttacgggacc
      301 agtgcctttc gcgtgaacta taacgcgaac gccaacaccg gtcgtaataa cacctcggcc
      361 tttggctcag cggacctgaa agccaatatc gggcgctggg tggtgagctc ttctgccacg
      421 gccagcggcg gtgacagcgg ggataactcc gccacgataa acatgttcac ggccacccgg
      481 gccatccgcg cactgagtgc ggacctagcg gtcgggaaaa catccaccgg ggacagtctg
      541 ctgggcagta cgggaacgta cggggtgtca ctgagccgga acaacagcat gaagccgggc
      601 aatctggggt ataccccggt gttcagtggc attgcgaacg ggccgtcgag ggtgacgctg
      661 acacagaacg ggcggttgct gcattcggag atggtgccga cggggccgtt ctccatcacg
      721 gatgtgccgc tgtacaccag tggtgatgtg accatgaaaa tcaccggtga agacgggcgc
      781 gagcaggtac agaacttccc gttgtcggtg atggccgggc agttaagtcc ggggcagcac
      841 gagttcagtg tggcagccgg tttgcctgac gatgacagtg acctgaaagg tggtgtgttt
      901 gcagcgtcat acggttacgg tctggacggg ctgacgctgc gcaccggggg ggtgtttaac
      961 caggactggc agggcgtcag cgctggggct gttctggggc tgagttacct tggagcggtg
     1021 tctgctgacg gggcttatgc cacggcgaaa taccgtgacg gcagccgcag cggaaataag
     1081 gtgcagttat cctggagtaa acaactggag atgacgaaca ccggactgcg ggtgagctgg
     1141 tcacggcaga gtgaggaata tgaggacatg tcctcctttg acccgacaga gttgtggtcg
     1201 cagtcaaatc atggtcgccg gacgaaggat gaatggaatg ccggtgtcag tcagccggtg
     1261 ggtggtctgt tcagtctgtc ggtgtccggc tggcagcgga gttattaccc cgcatccatg
     1321 accgggagtt accggtacag cgatgacaac ggtaaggaca cggggattac cggcaccctg
     1381 agcacacaga ttaagggtgt cagtcttaac ctgggctggt ccggttcacg gaacacccgg
     1441 ggggaaaaca actggtctgc atcggcgtca gtatcggtac cgttcactct gtttgaccgt
     1501 cgctacagca gcagtacgtc ggtgagtacg agtaaagacg gtggcacagg cttcagcacc
     1561 ggtgtgtccg gctcgctgaa tgaccgtttc agctatggcc tgggcggtgg gcgtgacagc
     1621 ggtggtggtg tgagcagtta tctgaatgcg tcgtacagtg gtgaccgggc ttatctgaat
     1681 ggtgccctga accactcgca gtccggcgga accagtggtt ctgtctcggt cagcggttcg
     1741 gtactggccg ttccggcggc gaaagacatt atgttcagcc gcacgaccgg ggataccgtg
     1801 gctgtggtga acgtgaagga tacccccgga gtgaaggtga cgtccggaga cggacagact
     1861 gacagcgacg gcaacctggt ggtaccgctg aacagctatg actggaacac ggtgacgatt
     1921 gatgcgggca cgctgccgct gagcacagaa ctgacgaaca ccagtcagaa ggtggtgcca
     1981 acggacaagg cggtggtgtg gatgccgttt gatgccctga aagttaagcg ttacctgctg
     2041 caggtgaagc agcgtgacgg tgagtttgtg tcggggggaa cctgggcacg tgacagtaag
     2101 aacacaccgc tgggctttgt agctaacaat ggtgtgctga tgattaacac ggtggatgcg
     2161 ccgggtgata tcaccctggg ccagtgccgg atacctgcgg cgaaactgca ggatactgag
     2221 aaactacagg agattacgtg tgagtaagcg taacgcagta acgacgtttt tcactaaccg
     2281 ggtgacaaaa gcactgggaa tgacgctggc gctgatgatg acctgtcaga gtgctatggc
     2341 ttccctagcg gcagatcaga cccgctatat ctttcgaggg gacaaggatg cgctgaccat
     2401 cacggtcacc aacaatgaca aggagcgtac ctttggtggt caggcctggg tggacaatat
     2461 cgtggagaag gacacccgtc cgacttttgt ggtgacaccg tccttcttca aggtgaagcc
     2521 gaatggtcag cagacactgc gtatcatcat ggcctcggac catctgccga aggataaaga
     2581 gtcggtgtac tggctgaacc tgcaggatat tccgccggct ctggagggca gcggtattgc
     2641 agtggcgctg cgcacgaagc tgaaactgtt ctatcgcccg aaggcactga ttgaggggcg
     2701 caaaggggca gaagaaggca tcagcctgca gagccgcccg gatggcagga ccatgctggt
     2761 gaacaccacg ccgtacattt ttgcgattgg cagcctgctg gacggaaacg ggaagagaat
     2821 tgccacggat aacgagacag cacagaaact gctgatgttc atgccggggg atgaagtaca
     2881 ggtgaaggga aatgtggtga aagtggattc tctgaatgat tacggtgaac tgcagacctg
     2941 gacgattaat cagaaaaaaa cgccaacgtc ttccgggcag aaggcatctg attcgctggt
     3001 taatccgtct gataaggcag ataaaaaata accgccctcc ggaacacgga acagaggggt
     3061 aataaatgaa gaaaacaatg atggcagtcg ccctggccct gagtgcactc agtattcagt
     3121 cagcacaggc cgctgaatac agggagaaaa ctcagtatct gggggtggtg aacggtcagg
     3181 tggtgggtaa cagtgtgatg aaggtgaccc gtacaccaac agacccggtg ctgtaccgga
     3241 gtggagacac gacgcctctt cccgacaacc tgactgtccg caacgcagag tccagggagg
     3301 catcaggcgg tctggcgtat atcacggtga agcaggtgct gccggataac ggggaagccc
     3361 gtatcaccct gaagacagcc ctgatggttg acggaaagaa agtggcactc agtgccagac
     3421 agcagggtga ggatgtggtg ataagcgtgc cggaggcaaa gaaacaggtt gagttaagaa
     3481 cggatgcacc ggcagagctg gaagtcccgg tcagttaccg gggaaatctg cagatagcac
     3541 tgcaggtgga agactgagaa tttatctccg acaacagatg caatacatcc ctgactccgg
     3601 aatgggtcat attggtaaag ggatttaccg tatttttaaa a
//
LOCUS       DAPHCV010000244         3528 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.249, whole genome shotgun sequence.
ACCESSION   DAPHCV010000244 DAPHCV010000000
VERSION     DAPHCV010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.249"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            238..1773
                     /locus_tag="RH033_004696"
     CDS             238..1773
                     /locus_tag="RH033_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDV2993754.1"
                     /translation="MSCIKEKSKYAFERAKQFYFMLWCKESLIYLLFSVLVTWCCGYT
                     VKNTLGNVLILFLMLLLGRQNVISATCVCIFLLLSACYAPVGMIYGKINNSFIASVMQ
                     TNINEAGEFITGIPVLYFIVSLVIAAFTFWFWRKRDKSHRGFIFLLFFIVLSVNAWPK
                     RMVTNVMTGIAETREEMMRYESLKNNQKDSWDIVSVEKKYKTIIIVIGESVRRDYLSV
                     YGYPLPTTPWLNSAPGIFINGYFSAAPNTIGSLSRTLTLDYTETGNPGNNIVTLARKA
                     GYETWWISNQGSLGQHDTLISVIAANADKKHFLKEGSSSSLRIDDEDMLQYVREAVNS
                     SSPKVIFIHMLGSHTNPCDRLFNDAQPFRELFGNKVSCYLSTLLKLDRFLQAIYNMTT
                     EHGDDFAMLYFSDHGQSVSSDSTPVHHAPGLRSGYDVPLMMMASDITEHHLNHVHISA
                     RRFPEIFQWITGIQTKNLPLSFSLLQNDFAESVSVFNGERDVPVMSLPEEPILTGNAE
                     SEK"
     gene            complement(1866..2108)
                     /locus_tag="RH033_004697"
     CDS             complement(1866..2108)
                     /locus_tag="RH033_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993755.1"
                     /translation="MSNEYQSTSINPDVFEQFLTLLNTHCKSSETALAMLSTLCYELM
                     RRSGIHSLDSLMLNGEGILIQLQGADIREKTGQHIH"
     gene            2440..2592
                     /locus_tag="RH033_004698"
     CDS             2440..2592
                     /locus_tag="RH033_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HDV2993756.1"
                     /translation="MPQRTFLMMLIVVCVTILCFVWMVRDSLCGFRVEQGNTVLVATL
                     AYEVKR"
     gene            complement(2907..>3528)
                     /gene="excA"
                     /locus_tag="RH033_004699"
     CDS             complement(2907..>3528)
                     /gene="excA"
                     /locus_tag="RH033_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HDV2993757.1"
                     /translation="PSWWAYYYLVRNAYFLFGVPFLIFCAIAFTALSLDIYSTKYDYI
                     YAYGSWTLLIIPSLWLYLKTRAKKKKILQVVKKIKESSPFAPTSDYEQLSFSKSCYFG
                     IDIKNGTMLYVRIYPNNVMDVIGLDIHNFTRTVAEDSKLEIHTTYVSLPMIPLEISGI
                     SSRTLANTMHTMAARGYEYKERFPQMIRYRVKEWEKVAGVPVAEVF"
BASE COUNT          945 a          682 c          831 g         1070 t
ORIGIN      
        1 gcctttccgg gagggcaaga catatttttg ttaactaaat gtattctgag tgttttatgg
       61 ttatgaggcc ttcatcgggt tatgcgaata atctggttcg gggagcagtt gctgtaaaca
      121 cagaacctga taatatgtca atcttgctgg acagaacagg atgtttttgt gagctgtttc
      181 atataaatat ccatgcgttg aaatgattgt ctgtgtttgt acaggaaaat gactggcgtg
      241 tcttgtatta aagaaaaatc aaaatatgct tttgagagag caaaacagtt ttattttatg
      301 ttgtggtgca aagagtcact gatttatctg ttgttttctg tgcttgttac atggtgctgc
      361 ggatatacag tgaaaaacac cctggggaat gtgcttatat tgttcctgat gttgttgttg
      421 gggcggcaga atgttatttc agcaacgtgt gtctgtatct ttcttttgtt aagtgcatgc
      481 tatgctccgg ttgggatgat ttacgggaaa ataaacaaca gttttattgc gtctgtaatg
      541 cagacaaata taaatgaggc aggtgagttt atcacaggca tacctgttct ttattttatt
      601 gtttcgctgg tcattgctgc ttttacattc tggttctggc gtaagcgtga taaaagccac
      661 cggggattta tctttctgtt gttctttatc gtattgtcag taaatgcatg gccaaaacgt
      721 atggttacga atgtaatgac ggggatagct gaaaccagag aagaaatgat gcgatatgaa
      781 tcgctgaaaa acaatcagaa agactcatgg gatattgtga gtgtggagaa gaaatataaa
      841 accataatta tcgtaattgg tgaaagtgtc agacgggatt atctttctgt ttatggctat
      901 cctttgccga caacaccatg gctgaattct gctccgggta tatttattaa tgggtatttc
      961 tcagcagcgc cgaatacgat aggttcactc agcagaacac tgacgcttga ttacactgaa
     1021 acagggaatc cgggtaataa tattgttacg cttgccagaa aagccgggta tgagacgtgg
     1081 tggatatcaa atcagggctc tttagggcaa catgatacgc tgatttctgt catcgccgcc
     1141 aatgcggata aaaaacattt tcttaaggaa gggagctctt cttcccttcg cattgatgat
     1201 gaggatatgc ttcagtatgt cagggaagct gttaactcgt cttctccaaa agtgattttt
     1261 attcatatgc tggggtctca cactaatccc tgtgaccgac tttttaatga tgcacagcca
     1321 ttcagggaat tattcggaaa taaagtatcc tgttatttat caacattgct taagctggat
     1381 cggtttcttc aggcaattta taacatgacg acagagcatg gtgatgattt tgccatgctg
     1441 tatttttctg atcatgggca atccgtcagt tcagacagca cccctgtgca tcatgcgcca
     1501 ggtttgcgtt cgggatacga tgtacctctc atgatgatgg ccagtgatat tactgagcac
     1561 cacctcaatc atgtgcatat cagtgcacgt cgttttcccg agatttttca atggatcaca
     1621 gggatacaaa ccaaaaatct gccgttaagt ttttctttgt tacagaatga tttcgctgaa
     1681 agtgtttctg tattcaatgg tgaacgtgac gttccggtaa tgtctttgcc agaagagccg
     1741 atactgaccg gaaatgctga atcagaaaaa taataaagat tatttatgac cgtgctcaat
     1801 gcctcatgtg tatgaggcat tgaacgttta tttcaggctg acactgtttt cagcacatcc
     1861 ctggatcagt gaatatgctg acctgttttt tcgcgaatat cagccccctg taattgtatc
     1921 aatatacctt ctccgttgag catcaggctg tccagtgaat ggatcccact cctcctcatc
     1981 agttcgtagc agagtgtact cagcatggca agtgcagttt ctgatgattt acagtgtgta
     2041 ttcaggaggg taagaaattg ttcaaaaaca tcagggttta tactggtgct ttgatattcg
     2101 ttactcatag tatttccttt caggtcatta ttacttccgg atttaacatg atgtctgtaa
     2161 ccatcaaata atgaacgtgt tccgggaatc cttgccgtgt agtttcccgt aaaaatactg
     2221 ttgtgcataa catgtcatta ggtggaaaac gattgtggca gactgaaaat cgattagaat
     2281 agcggcgggc gcttgaggct gtatgccgaa agcgttttgt ggacggcatg tagcagaaag
     2341 ccccaggcga ttttttatca atcaacccag ggcccactgt aatgcctaga caacattata
     2401 gtagcccgat gacggccgaa aggcaatgga ggggctgcga tgccacaacg aacgttttta
     2461 atgatgttaa tcgtcgtctg tgtgacgata ctgtgttttg tctggatggt gagggattcg
     2521 ctttgcggat tccgtgtcga gcagggaaac acagtgcttg tggcaacgtt agcctacgaa
     2581 gttaaacgtt aacgggcaac acggcggcag gaaatctgcc gccggattgt tgtgaaagca
     2641 aacctcaatg cgcccttttt tacctggatt tctgcagcgc gtttcagcac agcatgcagt
     2701 tctgcgctgc tccttgcttc cagtcgggca gtttgtgttg cagatggcat gatttgtacc
     2761 tcctagaata tactgaacgg caaattgccg tattgtgcta attacagtct gatacaggga
     2821 cggcagacag gcattttgcc tgaaaagaaa aagccggttc agagaaaacc ggctgacgga
     2881 ttaccgtcta acagttggtc gctaccttaa aatacttctg caacaggcac accggcaact
     2941 ttttcccatt ctttaacccg ataccgaatc atctgcggga aacgttcctt gtactcatat
     3001 ccacgtgcag ccatggtgtg catggtgttg gccagtgtcc ttgatgagat gccggagatt
     3061 tcaagaggga tcatcggcag actcacatag gtggtgtgga tttccagctt gctgtcttca
     3121 gcaactgttc gggtgaagtt gtgaatatcc aggccaatca catccatgac attgtttggg
     3181 taaatgcgca catacagcat ggtgccgttt ttaatgtcga tcccgaaata acaactcttc
     3241 gagaatgaca gttgctcgta atcgctggtc ggcgcaaagg gactgctttc tttgatcttc
     3301 ttgacaacct gcaggatttt ctttttcttc gctctggttt tcagatatag ccagagtgat
     3361 gggatgatga gtaacgtcca acttccatat gcatatatat aatcatattt agttgaatag
     3421 atatctagac ttaatgctgt aaaagcaatg gcacaaaaaa ttaaaaatgg aactccaaat
     3481 aaaaaataag cgttgcggac aaggtaataa tatgcccacc aggaagga
//
LOCUS       DAPHCV010000245         3442 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.250, whole genome shotgun sequence.
ACCESSION   DAPHCV010000245 DAPHCV010000000
VERSION     DAPHCV010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.250"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            59..1573
                     /locus_tag="RH033_004700"
     CDS             59..1573
                     /locus_tag="RH033_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2993758.1"
                     /translation="MTEENRRIKAITRFSYGSGNLIGSGALAISGAWLLYFYTTFCGL
                     TVFQATVIFSIATYLDVILNPLMGFITDNFYQTKLGRRFGRRRFFILIAIPCMIIYPL
                     LWVNGMSFWYYLSTYIIFEVVYTMIMIPYNTLPVEMTKDFNQRTYLTGSKAMFGKVAN
                     FLAAAIPGIYFYFFNKDSATPFFLTGLTYAIIMITSLIMLYFNSWERPTDDVVDESTH
                     GFMEGFNKLFTDLFSTFKLKTFRTHLGMYLFGFGAEWLFTATFTYFIVFNLEQPRTFV
                     SEMNSLSSICQFFSTAFFMMWCAKRGFKRPFIIAILIVVSSVIGYVGVYYLNIPHLTW
                     LIIGITIWFGLGTGGVYYIPWSVYVFLADVDEVLTGRRREGLYAGAMVMAGKLMRATV
                     VFCLGVVLSLFGFESGIHHQSASAVNAINGVMIFGVAGMALIGIYFSVKMKLDKASHA
                     EITKELERLKKGGRKEDATPEIRKLVKQLTGYEYDQCFGNNNVGFKFENKAGAF"
     gene            1575..>3442
                     /locus_tag="RH033_004701"
     CDS             1575..>3442
                     /locus_tag="RH033_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolygalacturonate lyase"
                     /protein_id="HDV2993759.1"
                     /translation="MNKHTLLLTVLFLNLICTPVFAQNWQVATFGQSTDLNFSSLIDS
                     AKIGRNNAWLAGNNNFLEAGKFYTLPTDFFIESRGGKIANSHDGMTVFYTIVPVTQTF
                     RLEADLTLEQIGPEVNGKSPAGQEGAGLFVRDIIGPQRQEPQSAGTEEYPQASNILMN
                     AFITQNKKNDNLVQITSIVREGVIKTWGNEGITIKKQPIIENINFTQKRNIHMTIERL
                     PEKFILTAFDTDRKENQSWQFSDYSGFMNQLDNNSLAIGFFAARNAKLRVKNASFKPG
                     KPLVDYKQLTSRQFSRVRHKAPELFLASPQSVVRNSTTLQFLANQAGIVSIDNDKQTK
                     QVQAGELVQFPVTLQKKHNDFTVNFNVDGNISKKAIRIEQVKSNLTDPYEIYVCSDCR
                     QGARGSKNDPVDLQTAVKFVAPGGNIYLNDGQYHGITLDRELSGIPGKYKTISAINPH
                     KAIFINKTFNLDASYWHLKSVVFDGNVDNGNNKPAYLRIAGSYNIIEHVIARNNDDTG
                     ISISAKDKNRFFWPAHNLVLNSDSYNNLDLSGINADGFAAKLGVGPGNIFRGCIAHNN
                     ADDGWDLFNKIEDGPNASVTIENSVAYENGLPYNKADILKGSIGNGFKLGGEGQPV"
BASE COUNT         1029 a          604 c          716 g         1093 t
ORIGIN      
        1 atactccatc ttttccagga gatggaaatg gacttttgtt gtggaagagg tatcataaat
       61 gactgaagaa aacagaagaa taaaagccat aactcgtttt tcatatggta gtggaaatct
      121 tatcggcagt ggagctctgg ctattagtgg agcatggtta ctttatttct acactacatt
      181 ttgtggattg acagtttttc aggccactgt aatattttcc attgctactt atctggatgt
      241 catcctgaat ccactaatgg ggtttattac ggataatttt tatcagacaa aactcggtcg
      301 tcgctttggt cgtcgtcgct tcttcatatt aattgccatt ccatgtatga ttatctatcc
      361 attgctttgg gtaaatggta tgagtttttg gtattatctt tctacttata tcatttttga
      421 agtggtatat actatgatta tgataccgta taatacctta cctgttgaaa tgacaaagga
      481 tttcaatcaa aggacctatt tgacaggttc taaagctatg tttggtaaag tagctaattt
      541 cttagcagcc gctattcctg gtatctattt ttacttcttc aataaagatt cagccacccc
      601 atttttcctg accggtttaa cttatgctat tattatgatc acttcattaa taatgctcta
      661 tttcaacagt tgggaacgtc caactgatga tgtagttgat gaatcaacgc atggttttat
      721 ggagggcttt aataaacttt ttaccgatct tttctcaacc tttaagctaa aaaccttcag
      781 aacccatctt ggtatgtatc tatttggttt cggcgctgaa tggctattta cagcgacatt
      841 tacctatttc attgttttca accttgaaca accgagaaca tttgtttctg agatgaactc
      901 attaagcagt atttgtcagt tcttctctac agcattcttt atgatgtggt gtgccaaacg
      961 aggatttaaa cgcccattta tcattgctat cttaattgtt gtatcttcag tgattggtta
     1021 tgttggtgtg tactatctta atattcccca tctgacctgg ctcattattg gcatcactat
     1081 ctggtttggt ctggggacgg ggggggtata ttacattccg tggagtgtat atgtattcct
     1141 tgccgatgtt gatgaggtct taactggtcg tcgtcgtgaa ggtctttatg ctggtgcaat
     1201 ggttatggcc ggtaagttaa tgagagccac tgtagtattt tgtctggggg ttgtcctcag
     1261 tctgtttggt tttgagtcag gcattcatca tcaatcagcc agtgcagtaa atgcaattaa
     1321 tggcgtcatg atatttggtg tggctggtat ggcgttaata ggtatctatt tctctgtaaa
     1381 aatgaaatta gataaagcat ctcacgccga aataactaaa gaattagagc gtctgaaaaa
     1441 aggtggcaga aaagaagatg ctacaccgga aattcgtaag cttgttaagc aactgactgg
     1501 ttatgaatat gaccagtgct tcggtaataa caatgttggc tttaaatttg aaaacaaagc
     1561 aggggcattc tagaatgaat aagcacacac tattactgac tgttcttttt ctgaatttga
     1621 tttgtactcc cgtttttgct caaaactggc aggtggcgac gtttggtcag tctacggatc
     1681 tcaacttttc atcgctgata gattcggcca agatcggacg gaataatgcc tggcttgcag
     1741 gaaacaataa ttttcttgaa gctggaaaat tttacacttt accaacagat ttttttattg
     1801 aaagccgtgg gggaaaaatt gctaactccc atgacggtat gaccgtcttt tatactattg
     1861 ttccggttac tcagacattc cgactggagg ctgatttgac attagaacag attggtccgg
     1921 aggtgaatgg aaaatcacca gcgggacagg agggagctgg attgtttgtc agagatatta
     1981 tcggtcctca gcgacaggaa cctcagtcag ctggaacaga agaatatccc caggcctcta
     2041 atatattgat gaatgccttt attacacaga ataaaaagaa tgataactta gtacagatta
     2101 cttcaattgt tcgtgaagga gtaataaaaa catggggtaa tgaaggtatt acaattaaga
     2161 aacagccgat cattgagaat ataaacttta cgcaaaaaag aaatattcat atgacgatcg
     2221 agcgactacc agagaagttc atcctgaccg cttttgatac cgatcgtaaa gaaaatcagt
     2281 catggcaatt ttctgattac tcaggcttta tgaatcaact ggataataat agtttagcta
     2341 ttggtttttt tgccgcacga aatgcgaaac taagggtgaa aaatgcatca tttaaaccgg
     2401 gcaagccact ggttgattac aaacaattaa cttcacgtca attcagtcgt gtccggcata
     2461 aagcccctga actttttctt gcttcacctc aatccgttgt aagaaactca acaactcttc
     2521 aatttttggc caatcaggct ggaatagtca gtattgataa tgataagcag actaagcagg
     2581 tgcaggcggg tgaactggta cagtttccag ttactttgca aaaaaaacat aatgacttca
     2641 ccgtcaactt taacgttgat gggaatatat caaaaaaagc tatacgcata gagcaggtta
     2701 aatcaaacct gactgatcct tatgagattt acgtatgtag tgattgtcga cagggggcca
     2761 gaggcagcaa aaatgaccct gtagatttac agacagccgt aaaatttgtc gcacccggcg
     2821 gtaatatata ccttaacgat ggtcaatatc atggaattac cttagatcgg gaattaagtg
     2881 gaatacctgg caagtataaa acaatttctg ccattaatcc acataaagcc atttttataa
     2941 acaagacatt caatctggat gcaagttact ggcatctaaa atccgtggtc tttgacggca
     3001 atgtggataa tggaaataat aaaccagcat atttgcgtat agctggtagc tataatatta
     3061 ttgagcatgt gatagccaga aataatgatg atacgggaat ttctatttca gcgaaagata
     3121 aaaaccgttt tttctggcca gctcataact tagttttaaa ctcagattca tataataatc
     3181 ttgatttatc cgggattaat gccgatggtt ttgctgcaaa attaggtgtc ggaccgggaa
     3241 acatttttcg aggatgcatt gcacataata atgcagatga tggttgggac ctatttaaca
     3301 aaattgaaga tggtccaaat gcatctgtta ctattgagaa ttctgtagcc tatgaaaatg
     3361 gcctgccata caataaagcg gatatcctaa aagggagtat tggcaatggc tttaaattag
     3421 gtggtgaagg tcaaccagta aa
//
LOCUS       DAPHCV010000246         3373 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.251, whole genome shotgun sequence.
ACCESSION   DAPHCV010000246 DAPHCV010000000
VERSION     DAPHCV010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.251"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(394..585)
                     /locus_tag="RH033_004702"
     CDS             complement(394..585)
                     /locus_tag="RH033_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HDV2993760.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNDLDADEQADICESLHDH
                     ADELYRSCLARFGDDGESN"
     gene            649..972
                     /locus_tag="RH033_004703"
     CDS             649..972
                     /locus_tag="RH033_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HDV2993761.1"
                     /translation="MLTIRVTDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPT
                     LAPPLLRHLAAIGNNLNQTARKVNSGQWSSIDRVHVVAALMAIEGELRQLRQAVREQG
                     VRDDS"
     gene            962..1681
                     /locus_tag="RH033_004704"
                     /pseudo
     CDS             962..1681
                     /locus_tag="RH033_004704"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1650..2210
                     /locus_tag="RH033_004705"
     CDS             1650..2210
                     /locus_tag="RH033_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HDV2993762.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLN
                     RQKIRDAISEHTTSVKKQLDTLSTTVSMQFSTTEAELSRQQKKLLWRVIKGRILFPAL
                     TALSVTGGIFLGCWGLMEWQESKIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKF
                     LVLPDGMKGENTWTVGDKNAVRLVRE"
     gene            2217..2450
                     /locus_tag="RH033_004706"
     CDS             2217..2450
                     /locus_tag="RH033_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HDV2993763.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHEVTQRNN
                     AILNERVVILSQQVQRLAGQVDRLSRLFIANNS"
BASE COUNT          817 a          814 c         1048 g          694 t
ORIGIN      
        1 aaaaaaaaaa acggatctgc atcacaacga gagtgatccc accgctcgcc gcagccccag
       61 cgaccgaacg tagtgagcga gtgggcgagg aagcggaaga gtcacagagg caagcattgt
      121 gcacttacgc gcctgtgcca gtgtccgcgt gtgtgttgtt tatcgggcgc gctgttttta
      181 cgcatctgtg cggcttttta ctcctgacat ggtgcacttc ccatccactc tgcaccgatg
      241 caaaatgagg taagccagta tacacatcgc tatcgctctg tgaactgggt cagggctgcg
      301 ccccgacacc cgcactgacg cgccctggcg ggcttgtctg ctcccggcat ccgctcacag
      361 gcaagctgtg aacgtctccg ggagctgcat gtttcagttg ctttcaccgt catcgccgaa
      421 gcgtgcgagg caactgcggt agagttcgtc ggcgtggtcg tgaagtgatt cgcagatgtc
      481 tgcctgttcg tccgcgtcca gatcgttgag tttttccagc agggttagcg tctggcttct
      541 gataaagcga gccatgttaa gggctgtttt ttcctgtttg gtcatggtct taaggctcct
      601 tgtatggggg atttgttagg gggaatgata ccgatgaaac gcgagaagat gctcacgata
      661 cgggttaccg atgaggagca cgcccggtta ctggaacgct gcgagggtaa acgactggcg
      721 gaatggatgc ggcgggtctg tctgggggag ccggttgcac gcacaggaaa actgccgaca
      781 ctggcaccgc cattgctgcg gcatctggct gccatcggga acaacctgaa ccagacagcc
      841 cggaaggtga acagcggcca gtggtcttcg attgaccggg ttcatgtggt ggccgcactg
      901 atggccatcg agggtgaatt gcgccagttg cggcaggctg tcagggaaca gggggtacgg
      961 gatgatagtt aaatttcatg ctagggggaa aggcggtgga agtggcccgg tcgattacct
     1021 gctggggcgg gagcgtaacc gcgaaggcgc aacggtgctc cggggtaacc cggaagaagt
     1081 gcgggaactc attgattcca cgccattttc gaagaaatac acgtcaggcg ttctgtcgtt
     1141 cgcggagaag gagctaccac cgggtgaacg cgaaagagtg atgacgagct ttgagcgtgt
     1201 tcttatgccc ggtctcgaaa aagaccagta cagcattctc tgggtggaac accaggataa
     1261 ggggcggctc gaactgaatt ttgtcatccc gaacatggag ctacagagcg gtaagcgcct
     1321 ccagccgtac tacgaccgtg cggacaggcc gaggataaat gcctggcaga cgctggttaa
     1381 tcatcattac gggctgcgtg acccgaacgc accggagaac agacggacgc ttgtcacgcc
     1441 aaataatctg ccaaaagcga aacaggaggc cgcagaagcg attacacggg gtcttgaggc
     1501 tctttaccat gccggagcaa taaaaacgcg tcaggatgtc acagaggcgc ttacagcggc
     1561 aggttttgag gtggtgagga caaccaaaag cagcatcagt attgccgacc cggagggggg
     1621 gcggaatctc aggctaaagg gggcgctgta tgagcaatct tttacaaatg ggaacagact
     1681 tcgagaagaa actgaaagag cgagccgctt ctaccgagaa catgctgaac agcgagttca
     1741 ggaagctcga agagtctgta gacaaggcat tgagcttaaa caggcagaaa atcagagacg
     1801 ctatcagcga acatacaaca tccgtgaaaa agcaactgga tacgctgagc accaccgtga
     1861 gcatgcagtt cagcaccaca gaggccgaac tgtcccggca gcagaaaaaa ctgctctggc
     1921 gggtgatcaa agggcggata ctgtttccgg cgctgaccgc gctcagcgtc acgggcggga
     1981 ttttcctggg gtgctggggg ctgatggagt ggcaggaaag caagattgcg aaaaatatcc
     2041 tgactatcag agagcaggaa aacacgctgg cgaaactcga ggcaaaaacc tggggggtga
     2101 cgttcgtcaa tggcgagaac gggaaatttc tggtgttgcc ggacgggatg aaaggggaaa
     2161 acacctggac ggtgggcgac aagaacgccg tgaggctggt cagggagtaa acgagtatga
     2221 cggaactgga agggcatttg ctgaacgcct tagagcattt gcaacaggac tatatgcggc
     2281 ggctgaacga gtgggagagc gccttcgcgg aattgcagaa gatgcacgag gttacgcaac
     2341 ggaacaacgc gatactgaac gagcgcgtcg tgatattgag tcagcaggtg cagagattag
     2401 ccgggcaagt ggatcgcttg agtcggttat ttatcgcgaa caacagctaa gggatgaaca
     2461 ggagcgtgcc cggtacagac atgaggtaca ggaacgcgcg gcacgggttc ggagtgggcc
     2521 atcaatggag atgtgaccgg ggaagtgacg aaatcttgcc attttgaccc gttcgacggc
     2581 tggggcgagc gaaacaaatc gtgtgccgta tttgtgcata gctatgcaac atcctgaaaa
     2641 cgcatttcac gcgtttttgg ggattcagcc aggggaaaac atggtttttt tactttttga
     2701 gcgtaaagac gtgaaaaatc tcgttattgc acttgcgatc tttcgtcggc gggcgtagaa
     2761 cgaaaacagc tcgccaggaa ggcgagctgt ttcgggttca ttgaggtctc tgacctcctc
     2821 aatgttcctg tggaaactgt tgagatccac aacagaaacc agttcattca gtttgcatga
     2881 gccttctgta ttcttcaaga atttttcaac agcacagtct cccaggaacc ccgaaaaaaa
     2941 cctgtttttg cagatacttg cgtatttgtg agaatcgttt gcggttgggg gagctgtgac
     3001 gacggcgacc gcacgcgtga gagttccgct accggcgcag agagtttcat cgacagcgga
     3061 cacctgatga gttgccacaa aaaccgtatg tagcgcggct agattggttg agttagacct
     3121 tggcggcagt tcccggtatg tggtggcgtg acgggtgaga tggtgcggcg aagacgcata
     3181 cgtcacgcag gcgcgggttc ttccactgac gcgccccgtg atcgatggcc ttggctccgt
     3241 ccggcatatc gagagcggca aactacccgt cccttcactg ggaggggtga gttgttgccg
     3301 ttccctccga ccttccacca tccagcatat cgcacgatcc catgtgcacc gctactcctg
     3361 ataactccag tgc
//
LOCUS       DAPHCV010000247         3334 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.252, whole genome shotgun sequence.
ACCESSION   DAPHCV010000247 DAPHCV010000000
VERSION     DAPHCV010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.252"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            124..579
                     /locus_tag="RH033_004707"
     CDS             124..579
                     /locus_tag="RH033_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993764.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKNVN"
     gene            680..888
                     /locus_tag="RH033_004708"
                     /pseudo
     CDS             680..888
                     /locus_tag="RH033_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001213776.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
     gene            989..1810
                     /locus_tag="RH033_004709"
     CDS             989..1810
                     /locus_tag="RH033_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDV2993765.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHHVPGIFGEDKHTSRSQN
                     YTYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSARGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            1810..2010
                     /locus_tag="RH033_004710"
     CDS             1810..2010
                     /locus_tag="RH033_004710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993766.1"
                     /translation="MKHIPEGMIQVPESPDAPEALPGCRSISDDDLCAWCTRLLYRPG
                     ELSLCRLSTGNGIWPSVCDRNG"
     gene            2148..2621
                     /locus_tag="RH033_004711"
     CDS             2148..2621
                     /locus_tag="RH033_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDV2993767.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDNDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            2637..3113
                     /locus_tag="RH033_004712"
     CDS             2637..3113
                     /locus_tag="RH033_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HDV2993768.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVSPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            3182..>3334
                     /locus_tag="RH033_004713"
     CDS             3182..>3334
                     /locus_tag="RH033_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 family protein"
                     /protein_id="HDV2993769.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGGTEAYTSREVQ
                     DIPGVLA"
BASE COUNT          791 a          920 c          874 g          749 t
ORIGIN      
        1 atggttaacc gactggaaga agcattaccc ccggagttac cggcataacg gctattctgc
       61 gccttttacg ttttatcacc tgatttgtca gtgtcatcgt catgactcag agaggtaaat
      121 accatgattc acctgttcaa aacctgcatg attaccgcct tcattctggg gttaacgtgg
      181 tctgccccac tccgggcaca ggatcaacgt tacatcagta tacgcaatac agatacgata
      241 tggctcccgg gaaatatttg tgcctaccag ttccggctgg ataatggcgg aaacgatgaa
      301 ggatttggcc ccctcaccat cactctgcaa ctcaaagaca aatatggtca gacgctggtg
      361 accagaaaaa tggaaacgga agcctttggt gacagtaatg ccacgcgaac cacagacgca
      421 tttctggaaa cggagtgcgt ggaaaatgtc gccacaaccg aaatcattaa agcaactgaa
      481 gaaagtaacg gccatcgtgt cagtctgccg ttatcggttt tcgatcccca ggactaccat
      541 ccactgctga ttaccgtttc cggaaaaaat gttaactgat ccctgaagtc agtttcatgc
      601 ttcagcctct gccacaacgt atggcagagg ctatttatca ggagaaacca gatatgccgg
      661 gttacacaca gagtatgtcc tggctgaagg atctttctca tacgggcaga cggtggctgt
      721 cacaacagct taccttaatg tgcttattga agatgaccag gtgcgcactt ccggctggtt
      781 atccgcaacg cggaaggtca gcgacgctgg cgctgccgga atactgaacc ctgtgccgga
      841 aagcagctta atgcgtggct cgccagtgaa gggcttctca gacaataaac gtcattgatt
      901 tcgtccatca ggccgcgtct tctccgggag acgcggcttt tttcatttat tacaccagta
      961 aatcttaacc accgataagg agcaaagtat gcgattagcc agtcgttttg gttatgctgc
     1021 aaaccagata cgccgtgacc gtccactgac acatgaagag ctgatgcacc atgtgccggg
     1081 gattttcggg gaagataaac acacgtcccg aagccagaac tatacgtaca tccccaccat
     1141 caccgtgctg gaaagtctgc agcgggaagg cttccagcca ttcttcgcct gccagacccg
     1201 tgtgcgcgac ccgggccgtc ggggatacac aaaacacatg ctgcgtctgc ggcgggccgg
     1261 agagataaac ggagaacatg tccctgaaat tattctgctc aactctcatg acggtacctc
     1321 cagctaccag atgctgccgg gttacttcag gttcgtctgc cagaacgggt gcgtctgtgg
     1381 tcagtctctg ggggaagtgc gtgttccgca ccggggaaat gtagtggaga aagttatcga
     1441 aggggcttac gaggtggtgg gggtttttga ccggatagag gagaagcgtg atgccatgca
     1501 gtcgctggtc ctgccgccac cggcacgcca ggcgctggca caggcggcac tgacttaccg
     1561 ttatggtgac gaacatcagc ccgtcaccac cgccgacatt ctgacgccac gacgccggga
     1621 ggattacgga aaggacctgt ggagtgctta tcagaccatc caggagaata tgctgaaagg
     1681 ggggatttcc ggtcgcagtg ccagaggaaa acgtatccac acccgtgcca ttcacagcat
     1741 cgacaccgat attaagctca accgcgcatt gtgggtaatg gcagaaacgc tgctggagag
     1801 tatgcgctga tgaaacatat ccctgaaggc atgattcagg taccagaatc acctgacgcc
     1861 ccggaggcgc tgccggggtg tcgttccatt tctgatgatg acctgtgcgc ctggtgtacc
     1921 cgtctgcttt accgtcccgg agaactcagt ctgtgcagac tgagtacagg caatggcatc
     1981 tggccatccg tctgtgaccg gaacggttag cgtacggctg tccggaattt cagccgaata
     2041 tcattcatcc ctgacattac cggctctcac ggtcagtgat gaacgtgatg ttgtcagccc
     2101 ccggccttta ctccctctca tttattcaca ttacaaggat ttcatacatg aaaacattgt
     2161 ctcagaacac cacctcttca gcctgtgcac cggagactgg cctgcaacaa ctggttgcca
     2221 ccatagtccc tgatgaacag cgcatcagct tctggccgca gcattttggc ctcattccac
     2281 agtgggtgac cctggagccc cgtgtcttcg gctggatgga ccgtctgtgc gaagactact
     2341 gcgggggcat ctggaatctg tacaccctga acaacggtgg cgcatttatg gcacctgaac
     2401 cggatgacga taatgacgag acatgggtac tgttcaatgc catgaacggt aaccgcgcag
     2461 aaatgagccc ggaagccgcc ggcattgcag cctgtctgat gacgtacagc catcatgcct
     2521 gtcgtacgga gtgttatgcc atgacggtcc attattaccg gctgcgggat tacgccctgc
     2581 agcatccgga atgcagcgcc attatgcgca tcatcgactg aatggaggca gggacaatgc
     2641 agcagctttc ctttctgccc ggagagatga cgcccggcga gcgcagcctc attcagcggg
     2701 ccctgaaaac tctggaccgc catcttcatg aacccggtgt ggccttcacc tccacccgtg
     2761 cggcacggga atggctgatt cttaacatgg cgggactgga gcgtgaagaa ttccgggtgc
     2821 tgtatctgaa caaccagaat cagttgattg ccggtgaaac cctcttcacc ggcaccatca
     2881 accgcacgga agttcatccc cgggaagtga ttaaacgcgc cctgtaccac aatgccgctg
     2941 ctgtggtgct ggcacacaat cacccgtccg gtgaagtctc acccagcaag gcagaccgcc
     3001 ttatcaccga acgtctggta caggcactgg gcctggtgga tatccgggtg ccggaccatc
     3061 tgatagtcgg tggcagccag gttttctcct ttgcggaaca cggactgctt taacccgtca
     3121 ccgtcacaac cacatcacac ctgttttcac ttttatcttc tgtcttcaga ggtatcacac
     3181 tatgaaaatc atcacccgtg gtgaagccat gcgtattcac caacaacatc ctgcatcccg
     3241 tctttttccg ttctgcacag gtaagtaccg ctggcacggt ggcactgaag cgtataccag
     3301 tcgtgaagtg caggatattc ccggtgtgct ggcc
//
LOCUS       DAPHCV010000248         3321 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.253, whole genome shotgun sequence.
ACCESSION   DAPHCV010000248 DAPHCV010000000
VERSION     DAPHCV010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.253"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            105..1655
                     /locus_tag="RH033_004714"
     CDS             105..1655
                     /locus_tag="RH033_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDV2993770.1"
                     /translation="MKTVFTTLSVIFGVIFSHSVLAQDVTIYYTNDIHAHVNPAKILA
                     VDKNRLIGGMANIAGIVNEAKKKSKDVFFFDAGDYFTGPYISTLTKGEAIIDIMNTMP
                     FDAVSVGNHEFDHGVPNMVSQLSKAKFPILLGNIYYTDTNKPVWDHPWTIIEKDGLKI
                     GVIGLHGAFAFYDTVAAKAREGVEARDEIKYLNKALAELKGKVDITVLLIHEGVPARQ
                     SSFGSKDVERLLQADIETAKKVNGVDVLITGHAHVGTPQPIKVNNTLIVSTDAYGTNI
                     GKLVLDYNPKTKKIDSYNGELITIFADQFKPDTIVQNTIDKWSAKLNKITQEVVGHSP
                     VVLTREYGSSSSTGNLILDAMMEKTPDAVAGFQNSGGIRADFPKGDITLGDVISTFPF
                     NNDLIEMDLTGRDLKSLMTHATNLTNGVLQVSKSVAVVYDSKKPLNQRLISFTINGKP
                     VEDNQTYRIATHSFCASGGDGFEAFLNGKNVKTIPGTTSAESIIDYFKNHKPVTPDLT
                     KRVMDVAK"
     gene            1696..3315
                     /locus_tag="RH033_004715"
     CDS             1696..3315
                     /locus_tag="RH033_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDV2993771.1"
                     /translation="MKTAQRTLFAATLLTIFSTSVMAQDVTIIYTNDLHAHVDSYKVP
                     YVADGKRDIGGFANISTLVKQEKAKNKATFYFDAGDYFTGPYISSLTKGEAIIDIMNT
                     MPFDAVSIGNHEFDHGWDNALRQLSKANFPVLLGNVYHKESEKPFWNKPYTILEKDGI
                     RIGIVGLHGVFAFNDTVSELSLQGLDNDNNNRFDKSSATLKNQGIEARDEVKYLQHYI
                     DELRDKVDLTVALVHEGVPARQSSIGNTDVRRALDKDIQTASKVKGLDILITGHAHVG
                     TPEPIKVGNTLILSTDSGGIDIGKLILDVNPTARTHKVKSFELKTVYADEWIPDPTTQ
                     KVINGWNKKLADLVRQPVGESSIALTRAYGESSQLGNLFTDAMLVAAPTAQIALINSG
                     SLRADINAGTITFGDITSTFPFKNELTEMDLSGKDLRNLLEHGASLTNGILQMSKGAE
                     MRYTLQKPVGQRIVSFKINGEEIVDTNIYHVATTTFLALGGDGFLAFKEGKNVQVRAG
                     NNMSNVVIDYFKKGHKITPAQVNEMRVEVSK"
BASE COUNT         1041 a          688 c          706 g          886 t
ORIGIN      
        1 gatcacataa tacttttctg aactatgctt acataaaata agacacgatg tgccttttat
       61 ttaaataatt caacaaatgt aacacaccct atccaggagg gatgatgaaa actgtattta
      121 caacattatc agtcatattc ggcgtgatat tttctcacag tgttctagct caggacgtta
      181 ctatttatta tacgaatgac attcacgcac atgtcaatcc ggcaaaaatc ctcgctgttg
      241 ataagaacag acttataggt ggtatggcta atattgcagg catcgttaat gaagcgaaga
      301 aaaaaagcaa agatgtgttt ttctttgatg caggcgacta ttttaccggg ccgtatatca
      361 gcaccctgac caaaggtgaa gctattattg atatcatgaa cactatgccc tttgatgcgg
      421 tgtctgttgg taatcacgag ttcgatcacg gtgtgcccaa tatggtgtct cagttatcaa
      481 aagcaaaatt tcccattctg ctgggaaata tctactacac cgatacgaat aaaccagttt
      541 gggatcatcc gtggaccatt atcgaaaaag atggtttaaa gattggtgtg attggattgc
      601 atggcgcatt cgctttctat gatacagtcg cggcaaaagc acgtgaaggc gtagaagcca
      661 gagacgaaat taaatattta aataaagcac ttgcagaatt aaaaggcaaa gtcgacatta
      721 ccgttttgct aattcacgaa ggtgtcccgg cgcgtcaatc aagctttggt agtaaagatg
      781 tggagcggct actacaggca gatattgaga cagctaaaaa agtgaatggt gttgatgtct
      841 taattaccgg tcacgcacat gtcggaacac cgcaaccaat aaaagttaat aacacattaa
      901 ttgtatctac cgatgcatac ggcaccaaca tcggaaaact tgtgcttgat tacaatccga
      961 aaacgaagaa aattgatagt tataatggtg agttaatcac catctttgca gatcaattta
     1021 aacctgacac catcgttcaa aatactattg ataaatggag tgcaaagctt aacaaaataa
     1081 cacaggaagt tgtcggccat tctcccgtag ttttaacacg tgagtatggt agttcctctt
     1141 ctaccggcaa ccttattctt gatgcaatga tggaaaaaac acctgatgcc gttgccggat
     1201 ttcaaaatag cggtgggatt cgagctgatt ttcctaaagg cgatatcaca ctgggagatg
     1261 ttattagcac attccccttt aataatgacc tcatcgagat ggatttgacg ggccgcgatc
     1321 tcaaatcatt gatgacgcat gcaaccaatc taactaacgg tgtgttacaa gtttctaaaa
     1381 gtgttgcggt tgtctatgac agcaaaaaac ccctcaacca acggttaatc tctttcacca
     1441 ttaacggcaa acctgtggaa gataatcaaa catatcgtat tgccacgcac tccttttgtg
     1501 ccagtggtgg tgacggtttt gaagcatttt tgaatggaaa aaatgtgaag acgataccgg
     1561 gaacaacctc ggcggaatct atcatcgatt atttcaaaaa tcataaacct gtcaccccag
     1621 acttaactaa acgagtcatg gacgtcgcca aataaaatct catttatcag tcaatttatt
     1681 taacaaggaa tgcgaatgaa aacagcgcag cgtactttat ttgccgcaac attgctgact
     1741 attttttcga cctcagttat ggcgcaggat gtcacgatta tttataccaa tgatcttcac
     1801 gcccatgtgg attcctataa agtaccatat gtcgcagacg gaaaacgcga cattggtggc
     1861 tttgccaata tatctacgtt agtaaaacaa gagaaagcca aaaataaagc cactttttat
     1921 tttgatgctg gagactattt caccgggcca tatatcagta gcctgacaaa gggcgaagca
     1981 ataattgata ttatgaatac catgcctttt gacgcagtat caatcggtaa tcatgagttt
     2041 gaccatggct gggataatgc cctccgacaa ttaagcaaag cgaatttccc tgttttatta
     2101 ggcaatgttt atcataaaga gagtgagaaa cccttctgga acaagcccta caccatcctg
     2161 gaaaaagatg gcatcaggat tggtattgtt gggctacacg gcgtatttgc atttaatgac
     2221 acggtctcgg agctctctct ccagggactc gataatgaca ataataaccg atttgataaa
     2281 tcttccgcga ccctcaagaa tcaggggatt gaagcacgtg atgaagtaaa gtatttgcag
     2341 cattatatcg acgagttacg tgataaggtt gacctcaccg tggcgctggt tcatgaaggc
     2401 gtaccggcgc gtcagtccag cattggcaat acagatgtta gacgtgcgct ggataaagat
     2461 attcaaaccg caagcaaggt gaaaggattg gatattctga ttaccgggca tgcccacgtc
     2521 ggaacgccag aaccaatcaa agtcggcaac accttaatcc tttcgactga cagtggcggt
     2581 atcgatattg gtaaattaat ccttgatgtc aaccctacag ctcgtactca taaagtgaag
     2641 agctttgagc tgaaaacggt ttatgccgat gaatggatac ccgatccaac cacacaaaaa
     2701 gttatcaatg gctggaataa aaaactggcg gatcttgtac gccaaccagt cggtgaatca
     2761 tcgattgcat taactcgtgc ctacggtgag tcatcgcaac ttggcaacct gtttaccgat
     2821 gcaatgcttg tcgctgcacc gactgcacaa attgcgttga taaactccgg cagtttacgt
     2881 gctgatataa atgcaggcac gattaccttt ggtgacatta ccagtacatt ccccttcaag
     2941 aatgaactta ctgaaatgga tctcagcggt aaggatctgc gtaacctact ggaacacggg
     3001 gcatcgctta ccaatggcat cctgcaaatg tcaaaaggcg cagaaatgcg ctataccctg
     3061 cagaaaccag taggtcagcg cattgtatct tttaaaatta atggtgaaga gatcgttgat
     3121 acgaatattt accatgttgc gactacgact ttccttgcac ttgggggcga tggttttctg
     3181 gcattcaaag aagggaaaaa tgtccaggtc cgcgccggaa ataacatgtc caatgtcgta
     3241 atcgattatt ttaagaaagg ccacaagatt actcctgcac aggtaaatga aatgcgggta
     3301 gaagtaagca aataacaaaa a
//
LOCUS       DAPHCV010000249         3270 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.254, whole genome shotgun sequence.
ACCESSION   DAPHCV010000249 DAPHCV010000000
VERSION     DAPHCV010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.254"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            278..943
                     /locus_tag="RH033_004716"
     CDS             278..943
                     /locus_tag="RH033_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993772.1"
                     /translation="METLENSERHWPARRKHMFFQIFMAQHICRDAVEIHWANGNIQV
                     IRPVRGISINGEAQGGIRPPYWVILAFCRSADGRIICSEGYAHALYQLTCPVPVDSKL
                     ERNTLTALLNVASWLKRKPGTPELSLERPLFDTEVYVNGEKKYVLPDFIVTARAPDGK
                     TARVVIETMGYEDSDYCARKSRQHTGMKQIGVLHTDPPKWLDNDHPPFEKHMYGVFMH
                     LRY"
     gene            1222..3102
                     /locus_tag="RH033_004717"
     CDS             1222..3102
                     /locus_tag="RH033_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin"
                     /protein_id="HDV2993773.1"
                     /translation="MSDPVRITNPGAESLGYDSDGHEIMAVDIYVNPPRVDVFHGTPP
                     AWSSFGNKTIWGGNEWVDDSPTRSDIEKRDKEITAYKNTLSAQQKENENKRTEAGKRL
                     SAAIAAREKDENTLKTLRAGNADAADITRQEFRLLQAELREYGFRTEIAGYDALRLHT
                     ESRMLFADADSLRISPREARSLIEQAEKRQKDAQNADKKAADMLAEYERRKGILDTRL
                     SELEKNGGAALAVLDAQQARLLGQQTRNDRAISEARNKLSSVTESLNTARNALTRAEQ
                     QLTQQKNTPDGKTIVSPEKFPGRSSTNHSIVVSGDPRFAGTIKITTSAVIDNRANLNY
                     LLTHSGLDYKRNILNDRNPVVTEDVEGDKKIYNAEVAEWDKLRQRLLDARNKITSAES
                     AVNSARNNVSARTNEQKHANDALNALLKEKENIRSQLADINQKIAEEKRKRDEINMVK
                     DAIKLTSDFYRTIYDEFGKQASELAKELASVSQGKQIKSVDDALNAFDKFRNNLNKKY
                     SIQDRMAISKALEAINQVHMAENFKLFSKAFGFTGKVIDRYDVAVELQKAVKTDNWRP
                     FFVKLESLAAGRAASAVTAWTFSVMLGTPVGILGFAIIMAAVSALVNDKFIEQVNKLI
                     GI"
BASE COUNT          960 a          691 c          801 g          818 t
ORIGIN      
        1 tatcagactg caggcccgct ctgcttacca taatggtgag agctcctccg ttcgtaagaa
       61 acgtccgcgc cttgcccgtt tactcctgtc tcttattgac gatgctgggc tgaataaact
      121 ggattccctg gctaatcccc gtatccggac taattatcgt gagtgtcttg atgccatacg
      181 gcaggttaca cggcagcagg agtatattcg gggaagagcc ttatctgaaa ttatccattt
      241 ccggcctgga atgtctgaaa ggtctcagga gcggttaatg gaaacgctgg agaattcaga
      301 acgacactgg cctgccagaa gaaaacatat gttctttcag atttttatgg cgcagcacat
      361 ctgtcgtgat gctgtggaaa ttcactgggc gaatggtaat attcaggtca tcagacctgt
      421 gcgggggatc agtattaatg gtgaagcgca gggcggaata cgtccccctt actgggttat
      481 tcttgctttt tgccggagtg ccgatggcag aatcatctgc agtgaagggt atgctcatgc
      541 tctttatcaa ctgacatgtc cggtgcctgt ggacagcaaa ctggaacgaa acacgctcac
      601 tgctcttctg aatgtggcca gctggcttaa aagaaagcca ggtacaccgg aattaagtct
      661 ggaaaggccc ctgtttgata cagaagttta tgttaatggt gaaaagaaat atgtcctgcc
      721 ggatttcatt gtcacagcaa gggctcctga cggaaagacg gccagagtgg tcatcgaaac
      781 gatgggatat gaagacagtg attactgcgc gagaaaatcc aggcagcata ccggcatgaa
      841 gcagattggt gttctgcata ccgatccacc gaaatggctg gataacgatc atcccccttt
      901 tgagaaacat atgtacggtg tttttatgca tctcaggtac tgagatattt tgtggctcag
      961 ttctgtaact tttcccgtaa cattgtctgt tgttacggga aagtccggtt tttgtattgc
     1021 accagagaat actcagactg tgatgctgcc acagcgtcag caggctttct gaacggtgta
     1081 acatcacttc atgttaatga taatcactat cattaaatct tgacatgcca ttttctcctt
     1141 aataaattaa tgctgtatat gtatccatat acgtaagcag ttaattcatt tgttttcctc
     1201 agaggatgaa ggagataccg aatgtctgac cctgtacgta ttacaaatcc cggtgcagaa
     1261 tcgctggggt atgattcaga tggccatgaa attatggccg ttgatattta tgtaaaccct
     1321 ccacgtgtcg atgtctttca tggtaccccg cctgcatgga gttccttcgg gaacaaaacc
     1381 atctggggcg gaaacgagtg ggtcgatgat tccccaaccc gaagtgatat cgaaaaaagg
     1441 gacaaggaaa tcacagcgta caaaaacacg ctcagcgcgc agcagaaaga aaatgagaat
     1501 aagcgcactg aagccggaaa acgcctctct gcggcgattg ctgcaaggga aaaagatgaa
     1561 aacacactga aaacactccg tgccggaaac gcagatgccg ctgatattac acgacaggag
     1621 ttcagactcc tgcaggcaga gctgagagaa tacggattcc gtactgaaat cgccggatat
     1681 gacgccctcc ggctgcatac agagagccgg atgctgtttg ctgatgctga ttctcttcgt
     1741 atatctcccc gggaggccag gtcgttaatc gaacaggctg aaaaacggca gaaggatgcg
     1801 cagaacgcag acaagaaggc cgctgatatg cttgctgaat acgagcgcag aaaaggtatt
     1861 ctggacacgc ggttgtcaga gctggaaaaa aatggcgggg cagcccttgc cgttcttgat
     1921 gcacaacagg cccgtctgct cgggcagcag acacggaatg acagggccat ttcagaggcc
     1981 cggaataagc tcagttcggt gacggaatcg cttaacacgg cccgtaatgc attaaccaga
     2041 gctgaacaac agctgacgca acagaaaaac acacctgacg gcaaaacgat agtttcccct
     2101 gaaaaattcc cggggcgttc atcaacaaat cattctattg ttgtgagcgg tgatccgaga
     2161 tttgccggta cgataaaaat cacaaccagc gcagtcatcg ataaccgtgc aaacctgaat
     2221 tatcttctga cccattccgg tctggactat aaacgcaata ttctgaatga ccggaatccg
     2281 gtggtgacag aggatgtgga aggtgacaag aaaatttata atgctgaagt tgctgaatgg
     2341 gataagttac ggcaacgatt gcttgatgcc agaaataaaa tcacctctgc tgaatctgcg
     2401 gtaaattcgg cgagaaataa cgtcagtgcc agaacaaatg aacaaaagca tgcaaatgac
     2461 gctcttaatg ccctgttgaa ggaaaaagag aatatccgta gccagcttgc tgacatcaat
     2521 cagaaaatag cggaagagaa aagaaaaagg gatgaaataa atatggtaaa ggatgccata
     2581 aaactcacct ctgatttcta cagaacgata tatgatgagt tcggtaaaca agcatccgaa
     2641 cttgctaagg agctggcttc tgtatctcaa gggaaacaga ttaagagtgt ggatgatgca
     2701 ctgaacgctt ttgataaatt ccgtaataat ctgaacaaga aatatagcat acaagatcgc
     2761 atggccattt ctaaagccct ggaagctatt aatcaggtcc atatggcgga gaattttaag
     2821 ctgttcagta aggcatttgg ttttaccgga aaagttattg atcgttatga tgttgctgtg
     2881 gagttacaaa aggctgtaaa aacggacaac tggcgtccat tttttgtaaa acttgaatca
     2941 ctggcagcag gaagagctgc ttcagcagtt acagcatgga cgttttccgt catgctggga
     3001 acccctgtag gtattctggg ttttgcaatt attatggcgg ctgtgagtgc gcttgttaat
     3061 gataagttta ttgagcaggt caataaactt attggtatct gacacataat gatgcccttt
     3121 cattttcctg aaagggcttt gcagaatata aaaattaagt aaatggcaac cacaggaact
     3181 gatataacga aacagaataa ttcaaatatt gcagttaagc ttccgccaac tggatttgta
     3241 aagaagtctt tttgccagag ttctttccct
//
LOCUS       DAPHCV010000250         3265 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.255, whole genome shotgun sequence.
ACCESSION   DAPHCV010000250 DAPHCV010000000
VERSION     DAPHCV010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.255"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..349
                     /locus_tag="RH033_004718"
     CDS             <1..349
                     /locus_tag="RH033_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV2993774.1"
                     /translation="AYVGGKNSASGFIASVLGGHNAITSETEWPTVSWESIIAANPDV
                     IVVASLDRNRWALDKAEEKIKFLKSDPAVSQLEAVKKGHIVVMDGQAMNPTIRTLYGA
                     EQVGEQLRKMGLN"
     gene            349..1386
                     /locus_tag="RH033_004719"
     CDS             349..1386
                     /locus_tag="RH033_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDV2993775.1"
                     /translation="MSVAAIETRRSILLTGWCVLAAIVLALVIAVGVSVGELAIPLQN
                     VFYAISNRTGLTAEPLNRIYESVIWDFRLSRALVAACCGAGLAICGVVLQSLLKNALA
                     EPYVLGVSAGASTGAVSIVVLGLGAGAISLSAGAFAGAFAAFAFVALLTNGARGGNER
                     TILAGVAASQLFNAITAYTISTSASAQQARDVMFWLLGSFSGVRWPEFQLVIVVVLAG
                     LAVCLWYARALDAFTFGDDAAASLGIAVPRVRLILFTTAALITATIVSMAGSIGFVGL
                     VVPHVMRFFFGPLHRTLLIASALAGAILMVLADIASRLLIAPQSLPVGVVTALVGVPF
                     FAVIIYRSRNK"
     gene            1386..2147
                     /locus_tag="RH033_004720"
     CDS             1386..2147
                     /locus_tag="RH033_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV2993776.1"
                     /translation="MSICAENITWKAGKKVIVNNVSLRVPRGETVGLLGPNGCGKSSL
                     LRVLAGLRRPDAGRVTLDGQDIARMAKKQLARRVAYVEQHGMTEANMRVRDVVRLGRI
                     PHHSPFSNWSAQDDEAIAAALQRVAMLEKSEQGWLSLSGGERQRVHIARALAQSPSEI
                     LLDEPTNHLDIHHQMQLMQLISELPVTSIVAIHDLNHAAMFCDSLIVMQQGQILASGT
                     PEEILSEALLWDVFRVKTKIEISPYHGKKHIHFIV"
     gene            2204..>3265
                     /locus_tag="RH033_004721"
     CDS             2204..>3265
                     /locus_tag="RH033_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2993777.1"
                     /translation="MLLGSQFVFNIGFYAVVPFLALFLRDDMLLSGGLIGLILGLRTF
                     SQQGMFILGGTLADRYGAKAIILAGCVVRVAGFLLLACGASLWPIILGACLTGVGGAL
                     FSPSIEALLARAGTHSQANGKRSRAEWFALFAVCGELGAVIGPVAGGVLSGIGFRHIA
                     LAGAGIFLLALAVLFFGLPADGHTTTTRRRVPWWTPLRQPRFVAFILAYSSWLLSYNQ
                     LYLALPVEIQRSGGREQDLAPLFMLASLLIITLQLPLARFARRMGAVRILPVGFLLLS
                     ASFASVALFAAAPPAESWLRLMPAAGFVTLLTLGQMLLVPAAKDLIPLFAEESTLGAH
                     YGALATAGGCAVLAGNLLLG"
BASE COUNT          514 a         1015 c         1032 g          704 t
ORIGIN      
        1 tgcctatgtc ggcggcaaaa acagcgcctc cggatttatc gccagcgtgc tgggcggaca
       61 taatgcgatt acctcagaaa ccgagtggcc cacggtgagc tgggagagca ttattgccgc
      121 caacccggat gtgatcgtgg tcgccagcct ggatcgtaac cgctgggcgc tggataaggc
      181 cgaggaaaag atcaaattcc tcaaaagcga tcccgccgtc agccagctgg aggcggtgaa
      241 aaaaggccat atcgtggtca tggacggtca ggcgatgaat ccgaccattc gcacgcttta
      301 cggcgcggag caggtgggcg agcagctgag aaaaatgggg ctgaactgat gagcgtcgcc
      361 gcgattgaga cgcgccgcag catactgctc accggctggt gtgtactggc agcgatcgtg
      421 ctggcgctgg tgatcgctgt gggcgtcagc gttggcgagc tggccatccc gctgcagaat
      481 gtcttttatg ctatcagcaa cagaaccggc ttaaccgctg agccgctcaa ccgtatctat
      541 gagagcgtta tctgggattt ccgcctcagc cgcgcgctgg tcgccgcctg ctgcggcgcc
      601 ggcctcgcta tctgcggtgt tgtgctgcaa agcttgctga agaatgccct cgccgaaccc
      661 tacgtcctcg gcgtctccgc cggggcctcc accggcgcgg tgtcgatcgt ggtgctgggc
      721 cttggtgctg gcgcaatttc cctctcggca ggcgcctttg ccggggcctt cgccgccttc
      781 gcctttgtcg ccttgctgac taacggcgcg cgcggcggca atgagcgtac gattcttgcg
      841 ggcgtcgccg cctcgcagtt atttaacgcc atcacggcct ataccatcag cacctccgcc
      901 agcgcgcagc aggcgcgcga tgtgatgttc tggctgctgg gcagcttcag cggcgtccgc
      961 tggcccgaat tccagttggt catcgtggtg gtgctcgcgg gtctggcggt ctgcctgtgg
     1021 tatgcccggg cgctggacgc gttcaccttc ggcgacgatg ccgccgcctc gctggggatt
     1081 gctgtgccgc gcgtgcgcct gatcctcttc accactgcgg cgctgatcac cgccaccatc
     1141 gtcagcatgg ccggctcaat cggctttgtt ggcctggtgg tgccgcacgt gatgcgtttc
     1201 ttctttggcc cactgcaccg aactttgctg atcgccagcg cgctggcagg ggcgatattg
     1261 atggtactgg cggacattgc gtcgcgcctg ctgattgccc cgcaaagtct gcccgttggg
     1321 gtagtaaccg ccctggttgg ggtgcctttc tttgccgtga ttatctaccg ctcaaggaat
     1381 aagtgatgag tatttgcgct gaaaatatca cctggaaggc aggcaaaaag gtcatcgtca
     1441 ataatgtctc gctgcgggtg ccgcgcggcg aaacggtcgg actgctgggg cccaacggct
     1501 gcggcaaatc ctcgctgctg cgcgttctgg cgggcctgcg ccgcccggat gcaggtcgcg
     1561 tcaccctcga cggccaggat atcgcccgga tggcgaaaaa gcagctcgcc cgccgcgtgg
     1621 cttacgtcga gcaacacggc atgaccgagg ccaatatgcg ggtgcgcgac gtcgtgcgcc
     1681 tgggacgcat tccccaccac tctccgttct caaactggag cgctcaggat gacgaagcga
     1741 ttgccgccgc gctgcagcgg gtagcgatgc tggagaaaag cgaacaggga tggttaagcc
     1801 tctccggcgg cgagcggcag cgggtgcata tcgcccgcgc gctggcgcag agcccgagcg
     1861 aaatcctgct ggatgagccg accaaccatc tggatataca ccatcagatg cagttaatgc
     1921 agttgatcag cgagctgccg gtaaccagca ttgtggccat tcacgatctt aaccatgccg
     1981 cgatgttctg cgattcgctg atcgtgatgc agcaggggca gatcctcgcc agcgggacgc
     2041 cggaggagat tttgtccgaa gcgctgctgt gggacgtttt ccgggtgaaa accaaaatcg
     2101 agatctcccc ttaccacggc aaaaagcaca ttcatttcat cgtttaggtt gggcggatat
     2161 gcctcttttc tccctgcgcc ctgcggcaac cctctggcct ccggtgttac tgggaagcca
     2221 gttcgttttt aatattggct tttacgcggt cgttcctttc ctcgccctct tcctgcgcga
     2281 cgacatgctg ctctcaggcg gacttatcgg cctgatcctc gggctgcgta ccttctccca
     2341 acaggggatg tttatcctcg gcgggacgct ggccgaccgc tatggcgcta aggcgatcat
     2401 tctcgccggc tgcgtcgttc gcgtcgccgg ttttctcctg ctggcgtgcg gggcgtcgct
     2461 gtggcccatt atccttggcg cctgcctgac cggcgtcggc ggcgccctgt tctccccgtc
     2521 gatagaggcc ctgctggcgc gggccggtac ccacagccag gccaacggga aacgcagccg
     2581 cgccgagtgg tttgcgctgt ttgcggtatg cggtgaactg ggagcggtga tcggcccggt
     2641 ggccggaggc gtgctgagcg ggatcggctt tcggcatatc gccctcgccg gcgcgggaat
     2701 atttcttctg gcgctggcgg tgctcttttt cggtctgccc gccgacgggc acacgacaac
     2761 aacgcgcaga cgagtcccat ggtggacgcc tttgcgccag ccgcgctttg tcgcgtttat
     2821 tctcgcctac agctcatggc tgttgagcta caaccaactg tacctcgccc tgccggtgga
     2881 gatccaacgt tccggcggcc gcgagcagga tctggcgccg ctctttatgc tggcctcgct
     2941 gttgattatc accctccagc tccctctcgc ccgcttcgcc cggcgaatgg gcgccgtgcg
     3001 catcctgccg gtgggctttc tgctgctgtc ggcttccttt gccagcgtgg cgttattcgc
     3061 cgccgcccca cccgccgaaa gttggctgcg gttaatgccg gcggctggtt ttgtcacgct
     3121 gctcaccctc ggccagatgc tgttagtgcc cgcagccaag gatctgatcc cgctgttcgc
     3181 cgaggagtcg acgctgggtg cccactacgg agcgctcgcc actgcaggag ggtgcgcggt
     3241 gttagccggg aatttactgc tcggt
//
LOCUS       DAPHCV010000251         3220 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.256, whole genome shotgun sequence.
ACCESSION   DAPHCV010000251 DAPHCV010000000
VERSION     DAPHCV010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.256"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            5..1924
                     /locus_tag="RH033_004722"
     CDS             5..1924
                     /locus_tag="RH033_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000558146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDV2993778.1"
                     /translation="MGLQAATSHSSMAKDTITIVATGNQNTVFETPSMVSVVTNDTPW
                     SQNAVTSAGMLKGVAGLSQTGAGRTNGQTFNLRGYDKSGVLVLVDGVRQLSDMAKSSG
                     TYLDPALVKRIEVVRGPNSSLYGSGGLGGVVDFRTADAADFLPPGETNGLSLWGNIAS
                     GDHSTGSGLTWFGKTGKTDALLSVIMRKRGNIYQSDGERAPNKEKPAALFAKGSVGIT
                     DSNKAGASLRLYRNNTTEPGNPTQTHGDSGLRDRKTVQNDVQFWYQYAPVDNSLINVK
                     STLYLSDITIKTNGHNKTAEWRNNRTSGVNVVNRSHTLIFPGAHQLSYGAEYYRQQQK
                     PEGSATLYPEGNIDFTSLYFQDEMTMKSYPVNIIVGSRYDRYKSFNPRAGELKAERLS
                     PRAAISVSPTDWLMMYGSISSAFRAPTMAEMYRDDVHFYRKGKPNYWVPNLNLKPENN
                     ITREIGAGIQLDGLLTDNDRLQLKGGYFGTDARNYIATRVDMKRMRSYSYNVSRARIW
                     GWDMQGNYQSDYVDWVLSYNRTESMDASSREWLGSGNPDTLISDISIPVGHRGVYAGW
                     RAELSASATHVKKGDSYQAGYAIHSFSLSYKPVSVKGFEASVTLDNAFNKLAMNGKGV
                     PLSGRTVSLYTRYQW"
     gene            1943..2734
                     /locus_tag="RH033_004723"
     CDS             1943..2734
                     /locus_tag="RH033_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HDV2993779.1"
                     /translation="MMKNTGYILALCLTASGHVLAHDVWITGKQAENNVIAEIGYGHN
                     FPSKGTIPDRRDFFETPRLYNGKETITLKPTSTDYVYKIESAGNGNSYVLSTKMKPGY
                     WSRTSSGWKPVSREGRNDVAYCEFVTKYAKSFIPGEQQMPAQLYQYPIGDELEIIPLS
                     DISRFGEDVKLKVLYKTSPLAGATLELDSVSYLKSSRHTHAAEHKHSAHKAELTFVSN
                     EDGIITVPSLHVGQWLAKVKNKKVFQDKNLCDETVDVATLSFSRN"
BASE COUNT          898 a          750 c          771 g          801 t
ORIGIN      
        1 cccggtggga ctacaggcag cgaccagtca ttcttctatg gctaaagata caatcaccat
       61 tgtcgcgaca ggaaatcaga acacggtatt tgaaacgccg tcgatggtca gtgtcgtcac
      121 gaatgacaca ccgtggagtc agaatgcggt tacatcggcc ggcatgctga aaggtgttgc
      181 cggtctcagc cagactggtg caggacggac caatgggcag acctttaatc tacgcggcta
      241 tgacaaaagc ggggtacttg ttcttgttga cggcgtccgc caactcagtg acatggcaaa
      301 aagcagtggc acttatctgg atccggctct tgtcaaacgt atcgaagttg tccgcgggcc
      361 aaactccagt ctgtacggca gtggcgggct gggaggtgta gtggacttca gaactgccga
      421 tgcagcagat ttccttcccc ccggagagac aaacggttta agtctgtggg gaaatattgc
      481 cagtggtgac cacagcacag gctcggggct cacctggttc ggtaaaaccg gaaaaacaga
      541 tgcgctcctt tctgtcatta tgcgtaaaag aggtaatatc tatcaaagtg atggtgagcg
      601 cgcacctaac aaggaaaaac ctgcagccct gtttgcgaaa ggctctgtcg gtataacaga
      661 cagtaacaaa gcaggtgcca gcttgcgtct ctaccggaat aacaccactg aaccgggcaa
      721 tcccactcag acacatggtg acagcggcct gcgtgacaga aaaacagtac aaaatgacgt
      781 acagttctgg taccagtacg ctcctgtgga taacagcctc atcaatgtaa agtcaacgtt
      841 atatctcagt gatatcacta tcaagacaaa cggtcacaac aaaacggcag aatggagaaa
      901 caacagaacc tccggtgtta atgttgtcaa caggagtcat actctgattt ttccgggagc
      961 ccatcagtta agttatggcg ctgaatatta ccgtcagcag cagaagccag aaggctctgc
     1021 cacactatat ccggaaggaa atattgactt tacatcgttg tatttccagg atgaaatgac
     1081 aatgaaaagc taccctgtta acattatcgt cggttcccgc tatgaccggt acaagagctt
     1141 caatccccgt gccggagaac tgaaagccga acgcctgtcc ccaagggcgg cgatttcagt
     1201 ctcaccgaca gactggctga tgatgtacgg ctccatatcc tctgcattcc gagcgcccac
     1261 aatggcagaa atgtacaggg atgatgtaca tttttaccgc aagggtaaac ccaattactg
     1321 ggttcctaac cttaatctga aaccagaaaa taacatcacc cgtgagattg gcgcaggtat
     1381 tcaactggat ggcctgctta cagacaatga ccggctgcag ttaaaaggcg gatatttcgg
     1441 aacggatgcc agaaactata ttgccacacg cgtggatatg aaacggatgc gttcttattc
     1501 ttataatgta tcccgggccc gtatctgggg atgggatatg cagggaaatt accagtctga
     1561 ttatgttgac tgggtgcttt cttataaccg gacggaaagt atggatgcca gcagcaggga
     1621 atggctgggc tccggcaatc ctgacacact tatcagtgac atcagcatac ctgttggtca
     1681 tagaggcgtt tatgccggat ggcgtgctga actttcagca tcagccacgc atgtgaaaaa
     1741 aggcgactcc tatcaggctg gctatgccat acattccttt tcactgtctt ataagcctgt
     1801 aagtgttaaa ggctttgagg cgtcagtaac tctggataat gccttcaaca agcttgccat
     1861 gaatggcaaa ggtgtgccgc tttcaggccg aactgtcagt ctttataccc gttatcagtg
     1921 gtaacctctc aggaaataaa tcatgatgaa aaatacaggc tatatcttag ctctttgtct
     1981 gacagcatcg gggcatgtcc tggcccatga tgtctggatt accggcaaac aggcagagaa
     2041 caacgttatc gcagagattg gttatggtca taatttcccc tcaaagggca cgattcctga
     2101 cagaagggat ttctttgaaa ctccccggct ttataacggg aaagagacaa taacgctgaa
     2161 gccaacatcc acggattatg tctataaaat tgagtctgca ggcaacggta atagctacgt
     2221 tctgtcaacg aaaatgaaac cgggatactg gtcgagaacc tcgtcaggat ggaagcctgt
     2281 cagccgggag ggcagaaatg atgtggcata ctgtgagttt gtgactaaat atgcaaaatc
     2341 ttttattcct ggtgaacagc agatgccagc acaactctat cagtatccaa taggggatga
     2401 gcttgaaatc attccgttat ccgatatcag tcgtttcggt gaagatgtga agctgaaagt
     2461 tctgtataaa acttccccgc ttgccggagc cactctggag cttgactctg tcagttatct
     2521 gaaatcatcc cgccatactc atgcggcaga gcataaacat tcggctcata aagcagaact
     2581 tacctttgta agtaatgagg acggtatcat cactgtaccc tcccttcatg tcgggcaatg
     2641 gctagctaaa gtcaaaaata agaaagtatt tcaggacaaa aacctgtgtg atgaaactgt
     2701 cgatgtggca accttaagct tctcccgaaa ttaataatac tattcctgcc ctgtaattta
     2761 cgggcagggt atatcaaccg gaaatagcct actgacatcc atgtataaag ccacataatt
     2821 attttgttga tgtatcttac ctaatacgta agcgtaaagc caatgccgtc tgtaactcct
     2881 gatccttgca gactaaatgt aagcgctcct tctgtgccgt cttgtggtgg ttgtcctgtc
     2941 tggcaacaat cgcgtccatc tattgatgga cacgaatgat gaattctcag acaacaaaag
     3001 atatcaccgg ctttcgctct tattttccag atgtattgcg actaatattc gaagagcaat
     3061 tatctgttcg ggctatcgca ctggagcagt taggcctcag tcattccacc gttcataata
     3121 tctttcaacg gtttaatgcc tctggcatgt aagcgtaaac cgaccgttgt attgacgctt
     3181 atttataggg gagaactacg ttccatggca ggagttcgtc
//
LOCUS       DAPHCV010000252         3209 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.257, whole genome shotgun sequence.
ACCESSION   DAPHCV010000252 DAPHCV010000000
VERSION     DAPHCV010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.257"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>3209
                     /gene="espP"
                     /locus_tag="RH033_004724"
     CDS             <1..>3209
                     /gene="espP"
                     /locus_tag="RH033_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter EspP"
                     /protein_id="HDV2993780.1"
                     /translation="GGLIAVSELSGRVSSRTTGKKKHKRILALCFLGLLPSSYSFASQ
                     MDISNFYIRDYMDFAQNKGIFQAGATNIEIVKKDGSTLKLPEVPFPDFSPVANKGSTT
                     SIGGAYSITATHNTKNHHSVATQNWGNSTYKQTDWNTSHPDFAVSRLDKFVVETRGAT
                     EGADISLSKQQALERYGVNYKGEKKLIAFRAGSGVVSVKKNGRITPFNEVSYKPEMLN
                     GSFVHIDDWSGWLILTNNQFDEFNNIASQGDSGSALFVYDNQKKKWVVAGTVWGIYNY
                     ANGKNHAAYSKWNQTTIDNLKNKFSYKVDMSGAQVATIENGKLTGTGADTTDIKNKDL
                     IFTGGGDILLKSSFDNGAGGLVFNDKKTYRVNGDDFTFKGAGVDTRNGSIVEWNIRYD
                     NKDNLHKIGDGTLDVRKTQNTNLKTGEGLVILGAEKTFNNIYITSGDGTVRLNAENAL
                     SGGEYNGIFFAKNGGTLDLNGYNQSFNKIAATDSGAVITNTSTKKSVLSLNNTADYIY
                     HGNINGNLDVLQHHETKKENRRLILDGGVDTTNDISLRNTQLSMQGHATEHAIYRDGA
                     FSCSLPAPMRFLCGSDYVAGMQNTEADAVKQNGNAYKTNNAVSDLSQPDWETGTFRFG
                     TLHLENSDFSIGRNANVIGDIQASKSNITIGDTTAYIDLHAGKNITGDGFGFRQNIVR
                     GNSQGETLFTGGITAEDSTIVIKDKAKALFSNYVYLLNTKATIEKGADVTTQSGMFST
                     SDISVSGNLSMTGNPDKDNKFEPSIYLNDASYLLTDDSARLVAKNKASVVGDIHSTKS
                     ASIMFGHDESDLSQLSDRTSKGLALGLLGGFDVSYRGSVNAPSASATMNNTWWQLTGD
                     SALKTLKSTNSMVYFTDSANNKKFHTLTVDELATSNSAYAMRTDLKNSDKLVVNKKLS
                     GKDNILLVDFLNKPSGEKLDIELVSAPGNSSKDVFKGSEQAIGFSNVTPVITTRETDD
                     KITWSLTGYNTVANKEATRNAAALFSVDYKAFLNEVNNLNKRMGDLRDINGEAGAWAR
                     IMSGTGSASGGFSDNYTHVQVGVDKKHELDGLDLFTGFT"
BASE COUNT         1018 a          674 c          718 g          799 t
ORIGIN      
        1 caggagggtt aatcgctgtt tctgaattat ccggcagagt atcatcaaga acaactggta
       61 agaaaaaaca caaacgcata cttgcattat gttttttagg cttattacca tcctcatatt
      121 cttttgcgtc acagatggat atttcaaatt tctacatccg tgactatatg gattttgcac
      181 agaacaaggg catatttcag gctggcgcaa caaatattga aatagtgaag aaagatggct
      241 ccaccctgaa actaccggaa gtaccatttc ctgacttctc accggttgca aacaaagggt
      301 caaccacatc tattggtggt gcatacagta tcacagccac acacaatacg aaaaaccacc
      361 actcagttgc gacgcaaaac tgggggaaca gcacgtacaa acaaactgac tggaatactt
      421 cacatcctga ttttgcagta tcccgacttg acaagtttgt tgttgagacc cgaggtgcga
      481 ctgaaggcgc agatatttcg ttatcaaaac agcaggcact tgaacgttac ggggttaatt
      541 ataaaggaga aaagaaactt atcgcattca gagccggctc tggtgtggta tccgttaaaa
      601 aaaatggacg cataactcca tttaatgagg tttcttataa gccagaaatg ttaaatggct
      661 ctttcgttca cattgatgac tggagtggat ggctgatatt aaccaacaac cagtttgatg
      721 agtttaataa cattgcctct cagggtgaca gcggttcagc actgttcgtc tatgataacc
      781 aaaagaaaaa gtgggttgtc gctggaactg tatgggggat ttataattac gccaatggca
      841 aaaaccacgc agcatacagt aaatggaacc agacaaccat tgacaatctg aagaacaagt
      901 tttcttacaa agtggatatg tcaggagctc aggttgcaac cattgaaaat ggaaaactga
      961 caggcactgg cgcagacacc accgatataa aaaataagga cttaatattt actggcggtg
     1021 gagatatcct cctgaaatcc tcttttgata atggtgctgg cggtcttgtc tttaatgata
     1081 aaaagaccta tcgagtaaac ggggatgatt tcacctttaa aggtgccggt gttgatacaa
     1141 gaaacggcag catcgttgag tggaatatcc ggtatgataa taaagacaac cttcacaaaa
     1201 ttggtgatgg cacattagat gtccgaaaaa cccagaacac caacctgaaa acaggtgagg
     1261 gtcttgtcat tcttggcgct gaaaaaacat tcaataatat ctacataacc agtggtgatg
     1321 gaactgtccg actgaatgca gaaaatgcac tgtctggcgg tgaatacaac ggtattttct
     1381 ttgcgaaaaa tggcggaact cttgacctga acggatataa tcagtctttc aataaaattg
     1441 ctgcaactga ttcaggtgct gtaataacca atacgtcaac caaaaagtcc gttttatccc
     1501 tgaataatac tgctgactat atctatcacg gcaacataaa cgggaatctg gacgtacttc
     1561 agcatcatga gacgaaaaaa gagaaccgtc gtcttattct tgatgggggg gtggacacaa
     1621 caaatgatat aagcctgcgc aatacacaac tgtccatgca gggacatgcc actgaacatg
     1681 ccatttatcg ggatggtgct ttctcttgtt cactaccagc gcctatgcgc tttttgtgtg
     1741 gcagtgatta tgttgcagga atgcaaaata cagaagctga tgctgtaaaa caaaacggaa
     1801 atgcctataa aaccaacaat gctgtctctg atttatcgca gccagactgg gaaaccggaa
     1861 cattcagatt tggaacgcta catcttgaaa attccgattt ttctattggt cgtaatgcaa
     1921 atgtaatcgg ggacattcag gccagtaaat caaacattac tattggtgac actacagcat
     1981 atattgattt gcatgctggt aaaaatatta ccggtgatgg ttttggcttc cgccagaata
     2041 ttgtgcgtgg aaactcacaa ggagaaacgc tgtttacagg agggatcaca gcagaagaca
     2101 gcactatcgt tattaaagat aaagcaaaag cattattttc aaattatgta tacctgctga
     2161 acacaaaagc aaccatagag aaaggtgctg atgtgacaac tcaaagtggt atgttctcca
     2221 cgagcgatat cagcgtctct ggtaatctgt ccatgacagg caatcccgac aaagacaata
     2281 aattcgagcc ctcaatatat ctgaatgatg cttcttatct actgactgac gactccgcca
     2341 gactcgttgc caaaaataaa gcatctgtgg tgggagatat acactccact aaaagtgcat
     2401 ccatcatgtt tggtcatgat gaaagcgacc tctcgcagtt gtctgacaga acctcaaaag
     2461 ggcttgcact tggtctttta ggtggctttg atgtctcata tcgcggttca gtcaatgccc
     2521 cgtcagcatc tgccactatg aacaacacct ggtggcaact aaccggagat tctgcgctga
     2581 aaacactgaa aagtacaaac agcatggtct atttcactga cagcgcaaac aataagaaat
     2641 tccatacgct gacggtcgat gaactggcaa ccagcaacag cgcctatgcg atgcgtacgg
     2701 acctgaaaaa ctcggataag ctggtagtaa acaaaaagct gtcaggtaag gacaatattc
     2761 tgttggttga ttttctgaac aaaccctccg gagaaaaact ggatattgaa ctggtgagtg
     2821 caccggggaa cagcagtaaa gatgtcttta aaggaagtga acaggcgata ggtttcagta
     2881 atgtaacgcc ggtcattaca acccgggaaa ccgatgacaa aataacatgg tcactgacag
     2941 gctataacac ggttgcaaac aaggaagcaa cccggaatgc cgccgccctg ttctctgttg
     3001 actataaagc gtttctgaac gaggtcaaca acctgaacaa acgtatgggt gacctgcgtg
     3061 acatcaacgg cgaagccggt gcatgggcac gcatcatgag cggtactggc tctgccagtg
     3121 gtggtttcag tgacaactac acgcacgttc aggtcggggt cgacaaaaaa catgagctgg
     3181 acggactgga tttgtttacc ggtttcact
//
LOCUS       DAPHCV010000253         3199 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.258, whole genome shotgun sequence.
ACCESSION   DAPHCV010000253 DAPHCV010000000
VERSION     DAPHCV010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.258"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(20..646)
                     /gene="dsbA"
                     /locus_tag="RH033_004725"
     CDS             complement(20..646)
                     /gene="dsbA"
                     /locus_tag="RH033_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HDV2993781.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKELTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(663..1649)
                     /gene="srkA"
                     /locus_tag="RH033_004726"
     CDS             complement(663..1649)
                     /gene="srkA"
                     /locus_tag="RH033_004726"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HDV2993782.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTPDQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1726..1995)
                     /locus_tag="RH033_004727"
     CDS             complement(1726..1995)
                     /locus_tag="RH033_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HDV2993783.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2065..2649
                     /gene="mobA"
                     /locus_tag="RH033_004728"
     CDS             2065..2649
                     /gene="mobA"
                     /locus_tag="RH033_004728"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HDV2993784.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDRKEAFINVNTPEELARWQEKR"
     gene            2631..3158
                     /gene="mobB"
                     /locus_tag="RH033_004729"
     CDS             2631..3158
                     /gene="mobB"
                     /locus_tag="RH033_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDV2993785.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPVLCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALMDINDVE
                     GLADFVVEWMQKQDG"
BASE COUNT          845 a          777 c          772 g          805 t
ORIGIN      
        1 ttatgtaatt cacaatgaat tattttttct cggacagata tttcacagta tcagcatact
       61 gctgaacaaa aacatccata ttgctggtat ccataccctg cggattcagc tgatatttac
      121 cgttaacaaa catcgccgga acaccacgca attgcacgtc agctgcggct ttttcctgct
      181 gagcgaccag agatttcacc acgaagctgt tccacgccgc gtcgtactct tcacctttaa
      241 tacctgcgtt gataaataca tcgcggatat cagatgctga acgaatggtc tgggttttct
      301 gtacgccttc aaacagcgga acagtgactt tgtcttccac gcccagcgcc atcgccacag
      361 cccatgcctg agtcagctct ttgcccaggt caccccccat gaagttgacg tggtatttag
      421 tcatcttcac gccttccggc agttttttct tcacgttatc agaaatatgc agaacttctt
      481 caaactgata gcagtgcggg cagaagaaag agaaaaactc cagcacttgc ggcgcgccag
      541 caactggttt ttccagggta gtgtactgtt taccatcttc atactgcgcc gccgatgcgc
      601 taaacgctaa aactaaacca gccagcgcca gccaaatctt tttcatgatc tactctctcc
      661 gattaataca taggtgttaa ttgcaaaggg ggttcttgta gaacttttgc ctgttctata
      721 aaagtcgccg tctgtcgcag ccagtaatct tccccggtta accacgggaa atttttcggg
      781 aacgcgggat cagcccaacg ccgcattagc caggcaagat aataaaccaa acgcatggcg
      841 cgtaaaggtt caatcagtcc gatttcagcg gtgtcgaact cgctaaattc ttcataagct
      901 tcaataatag tttccagttg catccgctgc tcggctttat cgccattgag caacatccac
      961 aaatcctgaa tagccggacc attacgtgca tcatccagat caacaaacat tggaccatcg
     1021 cgccagagaa tattcccggc gtggcagtct ccatgtagcc gcagaacggt gaaatcttcc
     1081 cgccagtgtg ctgtaacggc ggcgatcagc tcatctgtcg ctttcagaaa tgccgctttc
     1141 aacccggaag gtatcagtgt agcgtcctca aacagcttgc gaggctcaat gagatattca
     1201 ttcagaccga tggtcgggcg atggataaaa agctgtttgc gccccgtctg gtgcatacgc
     1261 cctaaataac gcccaaccgc ttccatctga tcgatattat cagcttcgaa ctggcgacca
     1321 ccgacgcttg gaaaaacagc gaaataaaat ccctgatgat tcaataaagt ctgaccgtta
     1381 aaggccacag gtgctgcgac cggaacttca tcatttacca actgcaacgc aaattgatgt
     1441 tcttcgagga tttgatcggg tgtccaacgt tcaggacgat aaaatttgac gacaaaacgt
     1501 cgacgatctt cgtcctgaaa ttgatagaca cggttttcat agctgttaag cggggtaaga
     1561 ccggaatcca cccggatccc ttgctcaaac agagcatcca tgatggtatc cgggtgtagt
     1621 gtctggaaag taaaagcgct gttgttcatc ccatcatccg gaaaacatta cgaatgatga
     1681 aggatatcat ttcacgatga ttttggtggc accgcttaca agcttttact ctttaattac
     1741 gccgcgagcg cgtaacagcg ccgttttgaa gtcttcttca taatctttct gcaaacccgg
     1801 gatcaccgca tctttcgcgg aatcacgcat tttcaggtga tagatcagaa tgtcatccgt
     1861 caaatccgcc agttcgccgt caaaacctga ctctttcgcc agtttctgca aaaattgcag
     1921 caggttaagg tctggctctt tttgccaggc tggctggagg agttcaataa cttcattcag
     1981 acgtttacat ttcatagtaa tgctccttac tcttgagaca gacacgttag cagggtcaat
     2041 cccacaataa aagaggcgat atcggtgaat ctgatgacga cgataacagg cgttgtgctg
     2101 gcaggcggta aagccagacg aatgggcggc gtagataaag gattgcttga attaaacggc
     2161 aaaccattat ggcaacatgt agctgacgcg cttatgacgc agctctctca cgtcgtggtt
     2221 aatgctaatc gtcatcagga aatctatcag gcaagcggtc tgaaagtgat tgaagattca
     2281 ctggcggatt acccaggccc tctggcagga atgctttcag taatgcagca ggaagcgggt
     2341 gagtggtttt tgttttgccc gtgcgatacg ccttacattc cccatgattt agccgcccgg
     2401 cttaatcatc agcgcaaaga tgcgcctgtc gtgtgggtcc acgacggtga acgcgatcac
     2461 ccgactattg ctctggtaaa ccgcgcaatt gagcctttat tactggaata tctgcaagca
     2521 ggagaacgcc gggttatggc atttatgcgt ctggctggcg gtcatgcggt tgatttcagc
     2581 gatcgtaaag aggcatttat aaacgtgaat acgccagagg agcttgcccg atggcaggaa
     2641 aaacgatgat accgttactc gcctttgccg cgtggagtgg tactggaaaa actacactgc
     2701 tgaaaaaact gatcccggta ttatgcgcca gagggatccg tccgggactg attaagcata
     2761 cgcaccatga tatggatgtt gataaaccgg gcaaagatag ctatgagttg cgcaaggctg
     2821 gcgcggcaca aaccatcgtt gccagccagc aacgatgggc cttgatgacg gaaacaccag
     2881 acgaagaaga gctggatcta cattttctcg caagtcgaat ggatacctca aagctggatt
     2941 tgattctggt cgaagggttt aagcatgaag agatcgcaaa gattgtgctg tttcgcgatg
     3001 gagccggaca tcgaccggaa gaattagtga tagacagaca tgttattgct gtagccagtg
     3061 atgtgccgct taatcttgat gtcgcgttaa tggatattaa tgatgttgag gggctggctg
     3121 attttgtggt ggagtggatg caaaagcagg acggataagc aaaagtaggc tggataagac
     3181 gcgccagcgt cgcatccgg
//
LOCUS       DAPHCV010000254         3157 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.259, whole genome shotgun sequence.
ACCESSION   DAPHCV010000254 DAPHCV010000000
VERSION     DAPHCV010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.259"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..296)
                     /locus_tag="RH033_004730"
     CDS             complement(<1..296)
                     /locus_tag="RH033_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDV2993786.1"
                     /translation="MMPEYQGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSA
                     DAAGIILTSLVINRQLWLYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAAL"
     gene            complement(832..1614)
                     /locus_tag="RH033_004731"
     CDS             complement(832..1614)
                     /locus_tag="RH033_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993787.1"
                     /translation="MNENTTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLVNRHGGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLAAVRDAVRSVSCRVAEDTRRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLAC
                     DVLRIPDEPPRQWFDRGVLREVVREARAEIRSMADALAKIRG"
     gene            complement(1660..2214)
                     /locus_tag="RH033_004732"
     CDS             complement(1660..2214)
                     /locus_tag="RH033_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HDV2993788.1"
                     /translation="MCLLNAWHARRCIAPIACSWRRIPKFWNWMRALTIEKETRMYGT
                     CETLCRELAAKYPGYTPLMLVIWSPEEIQALADGMDISLSDPEIRTVLARLEDIPEDQ
                     RIESGISSAAAMEIISNVSENRQVTVPAELLASLIQTAEQALWKREWAARDYGLAVPE
                     CVTRRQAVVNQARILLKNNTHENG"
     gene            complement(2108..2329)
                     /locus_tag="RH033_004733"
     CDS             complement(2108..2329)
                     /locus_tag="RH033_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993789.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LARQTLYRANRLFMEAYTEILELDACFND"
     gene            complement(2330..3013)
                     /locus_tag="RH033_004734"
     CDS             complement(2330..3013)
                     /locus_tag="RH033_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HDV2993790.1"
                     /translation="MSRFIQGDCVRVMATFPGNAVDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKTYT
                     SKAAYVGYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
BASE COUNT          664 a          953 c          911 g          629 t
ORIGIN      
        1 agtgccgcgt aaatcgcgtt gcattcgggg tgaaattcga tgtgacgcca caactgcgca
       61 tcgcgcatcc ggtaaagctg ggtcagtcct gcatccccgc tgtcgtggta cagccacaac
      121 tggcggttaa tcacaaggga agtcagaatg atgcctgcgg catcggcgct gaccgtgcgg
      181 tcaaaccagt tttcaccgtt gaccagatga aaacggtcgc catccggcat catgtaaccg
      241 ccaccgtccg gcaggcggat gaagtgccag aagcctccct gatattccgg catcattttt
      301 tgcgccagcg cgtacacata cgcctcggca tacagaaaat cattgccaaa caggtaaggc
      361 agaaaggaga gacggtcgca ctcttcaacg ttcagagtga caggtttcgc atattgcatg
      421 atgttttcct ccgttattca catattccgc gacaccgggc ggtgccgtgc ctccataaaa
      481 gccgggcgat aaggcgggat gcacagggcg caaaggaagc cgcagcctga cgcacacggc
      541 tggcgggagt tggagggtga gaccgcgaaa cctgagcggg tgaacgaccg gaaaatcacg
      601 gcggacggga aggaaggacc gggtgaccgc agcggcgaag cgtacccttg catccccgcc
      661 agcccggcta agatggaggc acggcaccgc ccgcacacga cacggcggcc acataactga
      721 aaacccacaa ggccatgcct gcatggccgg ataaagcgga gccggcggcg acttcctttg
      781 tgctctgtcc ggggaccggc agaaaaaaag cggggatttt ccccgccatc atcagcccct
      841 gatttttgcc agggcatccg ccatgctgcg gatttcggcc cgggcttccc ggacaacttc
      901 ccgcagaaca ccacggtcaa accactggcg gggcggttca tccggtatac gcagcacatc
      961 gcaggccagc ccactggaca ggatatgttc aatacaatcg ctgaccagat acatttccac
     1021 cgccgaaaaa cgtgcgcccc ggttcaggat ggcatcgagc accacgggca ggttttcgtc
     1081 gtcctcatcg agccagcagg ggtgggggtg atcgctttcc ttcacgacca ggcgaaagcg
     1141 gcgcgtctgg cgggtcagta acgtctgacc ggtggtggtc agcgccgccc ggatacgccg
     1201 cgtatcttct gccacccggc aactgacact gcgtaccgca tcgcgcaccg ccgcaagtac
     1261 agccctgatt tcgtcttcgg tcagccattc accggcatac ggaaaagaca cctgcgttcc
     1321 gtctgccgga agcaccggca gcgactgcca ctgactgccg gacagaacaa gttccgctcc
     1381 ggttccggtc agtttctgaa tggcggaggc caggtgcggc ggcagtacgc cgccgtggcg
     1441 gttaacgagt aacgtcgcca gccggggcgc atccagcaga acataaatgc tgatccagcc
     1501 cggatacttc gggttacgct ggtcgacatc cgtctcttcc ggccagccct gcgccagcag
     1561 caggttacgg gcgtgacgac aaatcagtgc gttcagcgtt gtgttttcgt tcatgattaa
     1621 tcgttctccc tgtatcgccg cccgcaggcg gcgataacat cagccgtttt cgtgtgtgtt
     1681 gtttttcagc aggatgcggg cctgattaac caccgcctga cggcgggtaa cgcattccgg
     1741 gacggcgagg ccgtaatcac gggcggccca ttcacgtttc cacagtgcct gttcggcggt
     1801 ctgaatcagg gacgccagca gttcagccgg gacggtcacc tggcggtttt cgctcacgtt
     1861 gctgataatc tccatcgccg cggcagaaga aataccggac tcaattcgct ggtcttccgg
     1921 gatgtcctcc aggcgcgcca ggacggtcct gatttcagga tcggacaggg aaatatccat
     1981 tccgtcagcg agggcctgaa tctcttccgg ggaccagata accagcatca gcggtgtata
     2041 tcccggatac tttgctgcca gctcccggca tagcgtttcg catgttccgt acattctcgt
     2101 ctccttctca atcgttaaag cacgcatcca gttccagaat ttcggtatac gcctccatga
     2161 acaggcgatt ggcgcgatac agcgtctggc gtgccaagcg ttcaacaagc acatcggaat
     2221 caaagcgaca gcgatgctcc atatcgttca gcctggcccg aaggtcacac atggtgttgg
     2281 ccacctccgc cacgtcggcg cgaagttttt catgtccggc atagttcatt tacgcagcct
     2341 ccggcataaa ccagtcatca tttgcggccc cctgctgcat ggcccgctgt acggcagcca
     2401 gacgttgctg tccggcacgg tgatactgtt caagcagctc gataccgata taccggcgtc
     2461 cggactgaag ggcggccaca caggttgagc cgctgcctgc aaacgggtcc agcacaatcg
     2521 cgttcgggtg tgtgaagctc tcaatcagtg gttgcaggct ggtaacaggc ttttccgtcg
     2581 ggtgatgacg attgcctgaa tatttccagc ccagcacgtc gggcaatggc ttctgaggca
     2641 gcgccggacg cccttttgcc aggatgtaag cgcattcgtg gcgatagccc acatatgcgg
     2701 ccttcgatgt gtaggttttg gtaaacacca ggtggccaac cacgctgaat cccgcatttt
     2761 tccaggcggc cataaagcga tcgacgcggt tccagccata gaagctcacc atcagcgcat
     2821 cttttttcag cacgcggtac atttcattac aggccggttg cagccattca tcggttttat
     2881 cgccggcgat ggtacgcccc tgacggtcac ggaaaccgac gagatatggc gggtcggtga
     2941 ggatgaaatc gacagcattg cccggaaacg tggccataac acgcacgcag tcaccctgga
     3001 taaaacggga cataacagca ctccttctct tacagagggc accgggaggg cgcccctgtg
     3061 gcgcccgttc cccgcggcct gtgggctgtt caccgcgtcc ggtaacgggt caggccgacc
     3121 gtggaatatc gcagccgtcg tctttttgac ggcgaga
//
LOCUS       DAPHCV010000255         3136 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.260, whole genome shotgun sequence.
ACCESSION   DAPHCV010000255 DAPHCV010000000
VERSION     DAPHCV010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.260"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            176..>3136
                     /locus_tag="RH033_004735"
     CDS             176..>3136
                     /locus_tag="RH033_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV2993791.1"
                     /translation="MNKVYSLKYCPVTGGLIVVSELASRVIKKTCRRLTHILLAGIPA
                     VYLYYPQISQAGIVRSDIAYQIYRDFAENKGLFVPGATDIPVYDKDGKLVGRLDKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYQSVSFGNGKNTYSLVDRNNHSSVDFHAPR
                     LNKLVTEVIPSAITSEGTKANAYKDTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAF
                     KTGGTTGVPLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDEQQKKWVIVA
                     VLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLPPLNWTYDKTSGTGTLS
                     QGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIVLKDSVTQGAGYLEFKDSYTVSAESGK
                     TWTGAGIITDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGTVVLNQ
                     QADTAGNVQAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQ
                     AADYGAVITNNAQQKSRLLLDLKAQDTNVSVPIGSISPFGGTGTPGNLYSMILNGQTR
                     FYILKSASYGNTLWGNSLNDPAQWEFVGTDKNKAVQTVKDRILAGRAKQPVIFHGQLT
                     GNMDVTIPQLPGGRKVILDGSVNLPEGTLSEDSGTLIFQGHPVIHASVSGSAPVSLNQ
                     KDWENRQFIMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRVFVDKNDGTGNYV
                     ILEEGTSVPDTVNDRSQYEGNITLDHNSTLDIGSRFTGGIEAYDSAVSITSPDVLLTA
                     PGAFAGSSLTVHDGGHLTALNGLFSDGHIQAGKNSKITLSGTPVKDTANQYAPAVYLT
                     DGYDLTGDNATLEITRGAHASGDIHASAASTVTIGSDTPAELASAETTASAFAGSLLE
                     GYNAAFNGAITGGRADVSMHNALWTLGGDSAIHTLTVRNSRISSEGDRTFRTLTVNKL
                     DATGSDFVLRTDLKN"
BASE COUNT          868 a          737 c          797 g          734 t
ORIGIN      
        1 aaaacaatac cgcatatgtg aaaaaaattg caggagcggt aattttgtaa gcaaacgaaa
       61 agtatttcac tatgtaacag acatcacaaa aatacattaa tgcagtcact atattttccg
      121 cccgggtgat aacgtctggt tatcattagt acttaaacat catggagagt ccgcagtgaa
      181 taaagtttat tctcttaaat attgccctgt caccggggga cttattgttg tctctgaact
      241 tgccagcagg gtaataaaaa agacatgccg aagattaacg catattcttc tggctggcat
      301 tccagcagtc tatctgtatt accctcagat atcccaggcg ggcattgtcc gttccgatat
      361 tgcctatcag atttatcgtg atttcgctga aaacaaaggg ctttttgtac ctggtgccac
      421 agatattccg gtatatgata aggacggaaa acttgtgggg agactggata aagccccaat
      481 ggccgatttc agcagtgtga gctcaaatgg cgttgctacg cttgtatcac ctcagtatat
      541 cgtcagcgta aagcataacg gaggatatca gagtgtgagc tttggtaatg ggaaaaatac
      601 atattccctt gttgaccgta ataaccactc ttctgttgac ttccatgctc cacgtctgaa
      661 taaactggtt acagaagtta ttccctcagc gataacatca gaaggaacca aagccaatgc
      721 ttataaagac actgaacgtt acaccgcttt ttatcgggtg ggtagtggta cgcagtacac
      781 taaggacaag gacggaaatt tagttaaggt tgccggcgga tatgctttta aaacaggagg
      841 aaccacagga gttcctctga tatctgatgc aacaatagtc tctaatcccg ggcaaaccta
      901 taatcctgta aacggacctt tacctgacta tggtgcccct ggggacagtg gttctccttt
      961 gtttgcttat gatgaacaac aaaaaaaatg ggttattgtt gctgtattaa gagcatatgc
     1021 aggtattaat ggtgctacga actggtggaa tgtcatacca acagattatc tgaaccaggt
     1081 tatgcaggac gatttcgatg cccccgtgga ctttgtttcc ggactgcccc ccctgaactg
     1141 gacatacgac aaaacatcag gcacaggcac cctgagccag ggcagtaaaa actggaccat
     1201 gcacgggcag aaagacaatg atctcaatgc cggtaaaaat ctggtattca gcgggcagaa
     1261 cggtgcaatt gtcctgaaag acagtgtgac tcagggggcc ggttatctcg aatttaaaga
     1321 cagttacacc gtatctgctg aatccggaaa aacctggacg ggtgccggca ttattactga
     1381 caaggggacg aatgtgacct ggaaggtcaa cggggttgcc ggtgacaacc tgcataaatt
     1441 gggggaagga accctgacca taaacggaac aggtgtaaac ccgggaggac tgaaaacggg
     1501 agacggtacc gttgtactta accagcaggc agacactgca ggtaatgttc aggccttcag
     1561 ttccgtgaac ctcgccagcg gacgaccgac cgtggtgctc ggagatgccc gtcaggtcaa
     1621 tccggataac atttcatggg gataccgggg aggtaagctt gaccttaatg gtaatgccgt
     1681 taccttcacc cgactgcagg ctgccgatta cggggcggtg attacaaata atgcacagca
     1741 aaaatcccgg cttttactgg atcttaaggc tcaggataca aatgtcagtg ttccgattgg
     1801 cagtatatcc ccctttggcg gtaccggcac accgggaaac ctatacagca tgatactcaa
     1861 cggccagacc cgcttctata ttctgaaatc tgccagctat ggtaacaccc tgtgggggaa
     1921 cagcctgaat gacccggctc agtgggaatt tgttggcacg gacaaaaaca aagcagttca
     1981 gacagtaaaa gaccggatcc tggccgggcg ggcaaaacaa cccgttatct ttcatggtca
     2041 gctgaccggg aatatggatg tcaccattcc acagctgccg gggggaagaa aggtcatcct
     2101 tgatggtagc gtgaacctgc cggaaggtac cctgagtgag gacagtggca ccctgatatt
     2161 ccaggggcat ccggttatcc acgcctccgt cagtggcagt gcgccggtca gcctgaacca
     2221 gaaagactgg gaaaaccgcc agttcataat gaaaacactg tcgctgaaag atgctgactt
     2281 ccatctttca cgtaacgcct cgctgaacag tgacattaag tcggataaca gccatatcac
     2341 actgggaagt gacagggtat ttgtggataa aaatgacgga acaggaaatt atgtcattct
     2401 ggaggaaggt acctctgtcc cggacaccgt gaatgacagg agccagtatg aagggaatat
     2461 tacgctggac cataactcaa ccctggatat cggcagccgg ttcaccggag ggattgaagc
     2521 ttatgacagt gccgtcagta tcacctctcc ggacgtcctg ttaacagccc cgggtgcttt
     2581 tgccggcagt tcactgacag tgcatgatgg cggtcatctt acagcactga acggtctttt
     2641 cagcgacggg catattcagg ccggtaagaa cagcaaaatc accctgagcg gtacaccggt
     2701 taaagatacg gctaaccagt atgcccctgc tgtatatctg acggacggat atgacctgac
     2761 cggcgataac gcaacactgg aaattacccg tggagcacat gcttccggtg atattcatgc
     2821 ctctgcggca tcaacagtta ccatcgggtc tgacacgccg gcagaactgg cttctgcgga
     2881 aacgactgca tcggcgtttg ccggcagtct tcttgagggc tataacgcag cattcaatgg
     2941 tgccataacc ggtggcaggg ctgatgtcag tatgcataat gcactgtgga ctctgggtgg
     3001 ggactccgcc atccacactc ttaccgtcag aaacagccgt atcagttctg aaggagaccg
     3061 tactttccgt accctgacgg tgaataaact ggatgcaaca ggcagtgatt ttgttctgcg
     3121 tacggacctg aaaaat
//
LOCUS       DAPHCV010000256         3129 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.261, whole genome shotgun sequence.
ACCESSION   DAPHCV010000256 DAPHCV010000000
VERSION     DAPHCV010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.261"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            64..186
                     /locus_tag="RH033_004736"
     CDS             64..186
                     /locus_tag="RH033_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993792.1"
                     /translation="MTKHPTGIYVGCFVKVIRRRLRLEQRRSVINYSPFVLQHP"
     gene            complement(152..1309)
                     /locus_tag="RH033_004737"
     CDS             complement(152..1309)
                     /locus_tag="RH033_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDV2993793.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQIEFNTLTVI
                     NNINVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHTENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKERISAKCQQCVYKPICNGGCPKHRIT
                     KVNNETISYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(1361..3043)
                     /locus_tag="RH033_004738"
     CDS             complement(1361..3043)
                     /locus_tag="RH033_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HDV2993794.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTYSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNIAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
BASE COUNT          860 a          640 c          684 g          945 t
ORIGIN      
        1 agggtattgc taatgccctg acgcatgccg cgtaagccag aattttcgca agggaagatg
       61 atgatgacga aacacccaac aggaatttat gtcgggtgct ttgttaaggt cataagacgg
      121 aggctaagac tggagcaaag aaggagcgtt attaattatt ctccatttgt tttgcaacat
      181 ccataatgtg gtaaagcggt actctgttct tggctaactc caccatggcg ttcatataag
      241 gtaccatggt tgaaaaaagg attttataac cttcgcaaaa ataggaaata gtctcattgt
      301 ttactttagt aatacgatgc ttagggcaac caccattgca gataggttta tatacacatt
      361 gctgacattt cgctgaaatc cgttcttttt gcgctgtcag ttgtacactg ttcatcgttt
      421 tgagttccga tttattaatg tttccaattt tgtactgtgg atagacaaaa tggtcgcatt
      481 cgtaaatgtc tccattactt tcaacaacca gattatcctt gcaggactcc tggaaaatac
      541 aactggtatg cccattcccc aaaaaacggc tgacaaagct ttcaaactga cggatgaaaa
      601 tttcacccac atcgttttta acccattgca taaaaatggt tgacataaac ttgccataag
      661 ccgtgggagg cacagaaaaa tcaatgatac ggaatgtatt ctcagtatga ccactgaaat
      721 caatattcgg cgtcccggtt tcgagcaatt cgataaattg catatgttta ctgccgatag
      781 attttaaaaa atgataaacc tcaagagggt aatggacatt aatgttatta ataacggtta
      841 acgtattaaa ctctatttga tatgatttca gacgctcgat ggctgctatc acttttgcaa
      901 aagtaccgtt acctgaatta ctgcgtctgt aacggtcatg tagctcttgg gggccatcga
      961 tcgagatacc aaccagaaac tcatgttctt tgagaaaggc acaccattca ttattcaata
     1021 aaatgccatt cgtttgtaat gcattaaaaa tacgtttttg gcctgcatag cgttgttgat
     1081 agtgaataac tttacggaaa aaatccaggc cagccagagt gggttcaccc ccttgccagg
     1141 taaaatagac ctgattgcca gacgctgcga tatattgttt gatgaactct ttcagagtgc
     1201 tgtcatccat ccatttttca tgagtaaact gcgactcttt ttcaaggtaa aaacagtaat
     1261 cacatttgag attacattga aaactggagg gcttggctgt aacgtgcatc gctatctcgc
     1321 tcaataaggc ggcggaaaaa tccgccgcat gaaggtttag ttatttcgct tcgcttagtg
     1381 ctttcttgat attgttaaac ttctcctgat ttacctcgct aagcggtggc tggctgctgt
     1441 cgataaactc tcttaccacg ccttgcatct ctttaacgac ctgcggattg gcggcggcaa
     1501 tgttatcttt ttgctgtaga tccgtcagtt tgtagagacc taactgattg ttttctactg
     1561 tatagacaag cgaataatcg ttatttctca ccgtataaga gaattggctt aagtcctcgg
     1621 tgttggggtt atgcgggtaa tcgtctgact gatggcggac aaatttgtgg taattatccc
     1681 agaagggaat attttcttca tcaaaccagt gagaataaga ggttatccag gtcagatttt
     1741 tatgtggctc gccttgtttc ttatcctgca accacggcag caaggaaacg ccatccagct
     1801 ttaggtcttt tggaatgctg atatcggctg catcaagagc tgtcgggtag aaatccattg
     1861 cggaaatcag cttgtcataa ttaccgggtt gaagttttcc tttccaccac ataaacattg
     1921 gggtgtgagt accgccagga taggtctgac tcttatagcc tttttgcgcc ccgttcagcg
     1981 gcagaggacc atcgataacc gcaccattat cggaggtaaa gagaataata gtattgtcat
     2041 actgtccgtt tttcttcagt tgttcgagaa tgcgttttac accctgatca acagaataaa
     2101 cggaagcgta gtagttatct gctgtttgac taccggtatt aaattgcttc tgatattgct
     2161 ccggtgcagg attatcattt ggcagatgcg gagcattata agccaggtaa agcataaaag
     2221 gctggtcaag tgttttggca cgatcaacaa cgccaattgc ctcatcggtt aactgatcgc
     2281 tgatataacc ttttgcgggg acgcgttcac ggtttttgaa cagtgaaggg gagttgtaat
     2341 atgccgttcc tgcagcgtgg aatcccataa agtaatcaaa gccacggttt tgcggttgcc
     2401 attcttccgc agaataggtg gtgaagttgt catgatagtc acgcgtttgt ttatcttccg
     2461 gtaccggcac attactgatt tttgacaagt gccatttacc tactgctgca gtgtaataac
     2521 catgattctg gaataattca ggcaggaaag tttctgttag cggaatacca tcctgagcat
     2581 cggtattgga atagacacca aagcgggcgg gcgctcgacc ggtcattatt gcggcgcggg
     2641 aggggccgga aacaccgtgc gccacatagc cgttagtaaa acgtacgcct tcatccatta
     2701 atgacaggag cgtcggcgtt gatttttgcg cagcttcaat ggctttatct atccctattt
     2761 tgtaggtatc gacaacttca cgattttcca ttgtttttgg gtcaaaagat cccttatcaa
     2821 aaggaagttg tccataacca agatcatcca tagtcagtac gataatattt ggctttccct
     2881 tggtactgta ttcagtcggc gtaaagtctg agaaagcaac gtttgttttg gttgctttca
     2941 gctttacatc atctgccgca tgagcagcaa atgcagccat accagatgcc agtatcaaag
     3001 atatcgaggt acttacgaca cttttcttta atgcagactt cataaatgtt cctcttctta
     3061 ttgtacttaa ttccaaagta attttacgaa gagaaataag tggatgtaag tgaagttagt
     3121 cacataaag
//
LOCUS       DAPHCV010000257         3080 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.262, whole genome shotgun sequence.
ACCESSION   DAPHCV010000257 DAPHCV010000000
VERSION     DAPHCV010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.262"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..182)
                     /locus_tag="RH033_004739"
     CDS             complement(<1..182)
                     /locus_tag="RH033_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV2993795.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGRVS"
     gene            complement(269..625)
                     /locus_tag="RH033_004740"
                     /pseudo
     CDS             complement(269..625)
                     /locus_tag="RH033_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(706..1479)
                     /gene="faeJ"
                     /locus_tag="RH033_004741"
     CDS             complement(706..1479)
                     /gene="faeJ"
                     /locus_tag="RH033_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbria accessory protein FaeJ"
                     /protein_id="HDV2993796.1"
                     /translation="MLNIIHRLKSGMFPALFFLTSASVLAQPLTILPGHWQEGMAVGV
                     TEFSGTLYVPEVSWQWQPRAVRMSTPDAVQAGLITGKSGMVSESRAGQDFYILGGHTT
                     SLTTARPGLQPSVTLLQVAPSSPRIAARGELARGQVRYGEITFTMRHLLAWQDDITAG
                     QGWSVVSGEVTPEAEKQVKRQLWQVNGYEWTPDYAGLTARPDAFISGAESLLSQENGS
                     QHIAGAWVTSLSDVRVNFPGAEEPVKRWQGNLTPVVVYF"
     gene            complement(1496..2260)
                     /gene="faeI"
                     /locus_tag="RH033_004742"
     CDS             complement(1496..2260)
                     /gene="faeI"
                     /locus_tag="RH033_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000758129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbria minor subunit FaeI"
                     /protein_id="HDV2993797.1"
                     /translation="MKKVTLFLFAASLLPSCVLAWNTPGEDFSGELKLEGPVTSTRNP
                     WVWKVGQGNEGLEVKQSRGVRDGEQAIPGALPAMTVLLGKTTLTTPAGREGLSPRVSY
                     GKGAEDFSLVWTAPGVAEVTLPVTGDNNVRAGTFVFRMQAAGVLRHVKDGQPAYAGVY
                     DDLNVNGLPGESAAMKTSDIPSVLQKMFSGEGPGWLQTMTISGYSGVSHFSDASLRQV
                     EGAYGAQTVAGSGELRLNGMMPERWRVSLPVSIEYQ"
     gene            complement(2292..3050)
                     /gene="faeH"
                     /locus_tag="RH033_004743"
     CDS             complement(2292..3050)
                     /gene="faeH"
                     /locus_tag="RH033_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbria minor subunit FaeH"
                     /protein_id="HDV2993798.1"
                     /translation="MISVVLFYSVAPHADILDGGEIQFNGFVTDESPKWTWQISSPDQ
                     TWVVDTADARTENGQLVFNLRDKGALPFLEGHLHEVAERGGPGFTPFITFSSNGQPFA
                     VKEGSGTTAQRFRASVPVRDPETGNVSGQLSFTLNQGVAVSAGAQEEGATVPAGMSLV
                     GGQSVTDVQSGTLPQGLKTRLSTLLLMNQGVGNGMNAVDNGQVISQGVLADGRVMNLA
                     AAYASVVSDFELRLPAEGTPAAWQAGLNVTVTVQ"
BASE COUNT          724 a          958 c          710 g          688 t
ORIGIN      
        1 gaaacacgcc cttcgttctg ccagagcctg agccatttga aaataacatt atcattgacg
       61 ccattttcac gtgcaatctg tgcaacacaa gctccaggtt gtgatgccag ttccaccata
      121 cgaagtttga attcattcga atagttttta cgaggttctt ttcgccagtc ctgtaattcc
      181 atgcttagat gtccgtctat atcagatggg cgtctaagtt accaattctc gtctgatggc
      241 tacatacggc ggtcagttta cgcttaccat taaccgactt tgcgcgcttg ctgacactgg
      301 tgtaatccgg gcagcgcaac ggaacattca tcagtgtaaa aatggaatca ataaaaccct
      361 gtgcagcccg cagggtcaac ctgaacacgc gtttaatgac cagaacggtg gtgatggcga
      421 gatcagaata gcgctgaggt cttccccgtg atgaaggcgt tgtcgactca taccaggcct
      481 gaatagcttc atcatccagc cagaaagtta tggagccacg gttgatgagg gctttattgt
      541 aggtgggcca gttggtgatt ttgaactttt gctttgccac ggaacggtct gcgttgtcgg
      601 gaagatgcgt gatctgatcc ttcaactcag caaaagttcg atttattcaa caaagccaat
      661 cacttactcc atatcatcgc aggcatcaca aagatgtcat tccgatcaga aatacaccac
      721 caccggcgtc agattcccct gccagcgttt taccggctcc tcagcaccgg ggaaattcac
      781 ccgaacatca ctcagggatg tcacccaggc accggcaata tgctggctgc cattctcctg
      841 tgacaacagc gactcagcac ccgaaataaa cgcatcagga cgtgcggtta agccggcata
      901 gtccggggtc cattcataac cgttcacctg ccataactgg cgtttcacct gtttttcagc
      961 ctccggcgtc acctctccgc tgaccacgct ccagccctga cctgctgtga tatcgtcctg
     1021 ccatgccaga agatggcgca tcgtgaacgt gatttccccg taacgcacct gtccccgggc
     1081 gagttctccc cgggccgcaa tacggggaga tgatggcgcc acctgaagta atgtcaccga
     1141 cggctgcagc cccggacggg cagttgtcag tgatgtggta tgtccgccaa gaatataaaa
     1201 atcctgccct gcccggcttt cactgaccat gccgcttttc cccgttatca gaccggcctg
     1261 cacggcgtct ggtgtactca tccgcacagc acggggctgc cactgccagg aaacttcagg
     1321 aacatacagc gtaccgctga attccgtcac gcccaccgcc attccttcct gccagtggcc
     1381 cggcagaata gtcaggggtt gtgccagcac actggctgaa gtcagaaaaa acagagccgg
     1441 aaacataccg gacttcagac gatgaataat attcagcacc ggaccgtcct cctgcttact
     1501 ggtactcaat acttaccggc agtgacaccc gccagcgctc cggcatcatg ccgttcagac
     1561 gtaattcacc actgcctgcc acagtctgtg caccatacgc accttcaacc tgacgcaggg
     1621 aggcatcact gaaatgactc actcccgaat aaccactgat ggtcattgtc tgcagccagc
     1681 cggggccttc gccactgaac atcttctgca gaacactcgg aatatcagaa gtcttcatgg
     1741 ctgcgctttc accaggcaga ccattcacat tcaggtcgtc atacacaccg gcatacgccg
     1801 gctgtccgtc cttcacatgg cgcaacaccc ccgcagcctg cattctgaaa acaaatgtgc
     1861 ctgcccggac gttattatct ccggtgacag gcagcgtcac ttccgccacg ccaggcgcag
     1921 tccagacaag tgaaaaatcc tcagccccct ttccgtaact gacccgggga gaaagtccct
     1981 cacgtcctgc cggtgtggtc agagtggttt ttcccagtaa aacggtcatc gccggcagtg
     2041 cgcccggaat tgcctgctca ccgtcacgaa caccgcggct ctgtttaacc tccagacctt
     2101 catttccctg tccgactttc catacccacg ggttacgggt gctggtcacc gggccttcca
     2161 gcttaagctc tccgctgaaa tcctctcccg gcgtgttcca ggccagtaca caggaaggca
     2221 ggaggctggc agcaaacaga aacaacgtca cctttttcac ttctcttctc tcctttatat
     2281 ccctgcaacc atcactgtac cgtgaccgtc acattcagcc ctgcctgcca tgctgcaggt
     2341 gtaccctccg ccggcaaccg cagttcaaaa tccgacacta ccgaggcata tgccgcagcc
     2401 agattcatca cccgaccgtc agccagtacc ccctgactga taacctgtcc gttatccacc
     2461 gcattcatac cattgccaac cccctggttc atcagaagta atgtggaaag gcgggtttta
     2521 agcccctgtg gcaacgttcc tgactgtaca tccgtcacgc tctgtccgcc gaccagcgac
     2581 atccctgccg gcactgtcgc cccctcctcc tgtgcaccgg cactgactgc caccccctga
     2641 ttcagggtga aagaaagctg tcccgacacg tttcccgtct ccgggtcacg caccggaaca
     2701 gaggcgcgaa aacgttgcgc cgtagtgcca ctgccttcct ttacggcgaa aggctgtccg
     2761 ttactgctga aggtaataaa gggggtgaat ccgggaccac cgcgctcagc cacctcatgt
     2821 aagtgcccct ccagaaacgg caacgcccct ttatcacgca aattaaaaac cagttgccca
     2881 ttctctgtac gcgcatcagc agtatccact acccaggtct ggtccggtga actaatctgc
     2941 caggtccatt tgggagattc gtcagtgaca aaaccattaa actgaatttc gccgccatca
     3001 agaatatctg catgtggcgc taccgaataa aacaggacca ccgaaataat ggcagaaaga
     3061 aaggatttat aatgatgcgt
//
LOCUS       DAPHCV010000258         3050 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.263, whole genome shotgun sequence.
ACCESSION   DAPHCV010000258 DAPHCV010000000
VERSION     DAPHCV010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.263"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            194..1045
                     /locus_tag="RH033_004744"
     CDS             194..1045
                     /locus_tag="RH033_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDV2993799.1"
                     /translation="MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINTGYRLID
                     TAASYQNETQVGNALKRSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVD
                     LYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEV
                     NPFNQQLQAVPWMQSRGIQPEAWAPFAEGKNGLFQHPVLTAIGEKYGKSVGQVVLRWI
                     YQRGVVSLAKSVRKERMEENINILDFELSPEDMLQITALDTATSAFFSHRDPAMVEWL
                     TGRKLDV"
     gene            1072..2061
                     /locus_tag="RH033_004745"
     CDS             1072..2061
                     /locus_tag="RH033_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDV2993800.1"
                     /translation="MQKRYLGKSGLEVSALGLGCMGLSHGYGPATDTHQAIELIRAAV
                     ERGVTFFDTAEVYGPYLNEEVVGEALKPFRDRVVIATKFGFTFGDDNKQQILNSRPEH
                     IREAVEGSLRRLKTDVIDLLYQHRVDPDVPIEDVAGTVKDLIAEGKVKHFGLSEAGAQ
                     TIRRAHAVQPVTALQSEYSMWWREPEQEILPLLEELGIGFVPFSPLGKGFLTGAIKPG
                     TTFGKDDYRSTVPRFAAQAIEANEKLVTLLGELAAEKGVTSAQIALAWLLAQKPWIVP
                     IPGTTKLNRLEENLAAADIVLSQKDTQQITEALETIKIVGERYSPEHQARVGR"
     gene            complement(2092..2985)
                     /locus_tag="RH033_004746"
     CDS             complement(2092..2985)
                     /locus_tag="RH033_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2993801.1"
                     /translation="MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAIKALEE
                     RLNIRLLTRTTRSVAPTEAGERIIACLEPRLDELEQELESLIQLNGTPSGNIRLSAGE
                     HAARSLVWPKLKPFLREYPEINVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
                     GPDMRMAVVGAPAYFAANPAPETPHELQNHRCINMRLPTAGGLYHWEFEREGKPLRVK
                     VDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYIKSGKLIQVLQEWCPTFPGYYLYY
                     PSRKQHPPAFALLIDALRYTE"
BASE COUNT          794 a          785 c          736 g          735 t
ORIGIN      
        1 caatatattt tttatggata ttcattttcc ttcatcagac tgtgaattgc agggatatcg
       61 aacttgcacg actcatcgcc acattcaaac agatttatga atttcgttca taacccctga
      121 caaatcctgc cgtctaatcg agcttttccc tttacgttat gcttctcatg aacgataacg
      181 gaaggataac accatgcaaa aagtaaaact gaacaacggt attgaaatgc ccctgttggg
      241 ctttggtgta tttcagatga ccgatgccgc tgaatgcgaa agagcggtca ttgacgctat
      301 taacaccggt tatcgactga ttgacaccgc cgcctcctat caaaatgaaa cccaggtcgg
      361 gaacgcactg aaacgaagcg gtatcgcccg taacgaactc tttgtcacca caaaactctg
      421 gttgcaggat accagttacg aaggggccaa agcccagttc gaacgctctc tgaatcgact
      481 gcaacttgat tacgttgacc tgtatttgat tcaccagcca tacggcgatg tccacggagc
      541 ctggcgtgcc atggaagagc tgcaacaggc cggtaaaatt cgcgctattg gcgtcagtaa
      601 cttccatcct gaccgactgg ctgaccttat cgcttttaat aacgtcgttc cggcggtaaa
      661 ccaggttgaa gttaaccctt tcaatcagca gctacaggcc gttccctgga tgcaaagtcg
      721 tggcattcag ccagaagcct gggcaccgtt tgcggaaggg aaaaatggtc tgttccagca
      781 tcctgttctc acggcaattg gcgagaagta tggcaaaagc gtgggccagg ttgttctgcg
      841 ctggatctac caacgaggcg ttgtttcact ggcaaaatca gtgcgaaaag aacgcatgga
      901 agagaacatc aacattctcg attttgaact cagccctgaa gatatgctgc aaattaccgc
      961 cctggatacc gcaaccagcg cgttcttctc tcaccgcgat cccgcaatgg tggaatggct
     1021 gactggccgc aaacttgatg tttaagccgc gataaaagag gtattcattc aatgcaaaaa
     1081 cgttatctgg gtaaatccgg actcgaagtc tccgcccttg ggctcggttg catgggctta
     1141 agccacggct acggcccggc gaccgatacc catcaggcta tcgaacttat tcgtgctgcg
     1201 gttgaacgtg gcgtcacctt cttcgatacc gccgaagtgt atggtccgta tctgaatgaa
     1261 gaagtggtcg gcgaagcctt aaaaccgttt cgtgaccgcg tggtaattgc cactaaattc
     1321 ggttttacct tcggcgatga taataagcag cagattttaa acagccgtcc ggaacatatc
     1381 cgtgaagccg ttgaaggatc attacgtcgt ctgaaaacag atgttattga cctgctgtat
     1441 caacaccgtg ttgatccaga tgttcctatt gaagatgttg caggaacggt gaaagacctc
     1501 atcgcagaag gtaaggtcaa acatttcggc ctgtctgaag caggagcaca aaccattcgt
     1561 cgtgcacatg cggtacaacc tgtcaccgcc ctgcaaagcg aatattccat gtggtggcgt
     1621 gagcctgaac aggaaattct gccgttgctg gaggaactgg gaattggttt tgtccccttc
     1681 agcccactag gtaaaggttt cctgacaggg gcgattaagc cgggaaccac tttcggcaaa
     1741 gatgattacc gcagcaccgt accgcgtttc gcagcacaag ccattgaagc caatgaaaaa
     1801 ctggtcacct tgttaggtga actggctgcg gagaaagggg taacgtctgc acaaatcgca
     1861 ctggcatggc tattagcaca aaagccgtgg attgttccca tccctggcac cactaaactg
     1921 aaccggctgg aagaaaacct ggccgctgct gacattgttc tttcgcagaa agatacgcag
     1981 cagataaccg aggcgctgga aacaattaaa atcgtcggtg aacgttactc gcctgaacat
     2041 caggcacgcg taggccgcta atacccaaac gggtccagga taaaggaccc gttattcggt
     2101 gtagcgaagc gcatcgatca acagcgcaaa agctggcgga tgctgcttgc ggctggggta
     2161 gtaaagataa tacccaggaa aagttggaca ccactcctgt aacacctgaa taagcttgcc
     2221 tgactttata tactcctgaa ccatgtcttc agggacacaa gcgatcccaa accctgataa
     2281 cgcggcatca attctttctg gcaatggact acacgtcagt tgcccatcaa ccttaacgcg
     2341 aagcggtttc ccttctcgct cgaactccca gtggtaaagc ccaccggcag ttggcaaacg
     2401 catgttgatg cagcgatgat tttgtagctc atgaggcgtt tcaggcgcag ggtttgcagc
     2461 aaaatacgcc ggagctccca ccacagccat tcgcatatct ggtccaatcc tgactgcaac
     2521 catatcttta tcaacatttt cacccagtcg tatcccggca tcaaaacggc cttcaacaat
     2581 atcgacaaaa ccgttatcca ccaccagttc gacattgatt tcgggatact ctctgagaaa
     2641 aggttttagc ttcggccaga ccagacttcg cgcggcatgt tccccggcag ataaccgaat
     2701 attcccggag ggcgtgccgt tcaattgaat aagtgattcc agttcctgtt caagctcgtc
     2761 aagacgcggt tcaaggcagg caatgattct ctcccccgct tctgtgggag ccacactgcg
     2821 ggtagtccgt gtcaggaggc ggatattgag cctttcctcc agagccttta ttgcatggct
     2881 aagtgcagac tgagagacgc cgagtttgcc cgctgctttg gtaaaacttc gctctcttgc
     2941 caccacaaga aagatttgca gttcgttgaa gttttctttg agcattggcg atttccttgt
     3001 agaagcatcc tcttcacaca cactcatgaa gggggcatag atgctattat
//
LOCUS       DAPHCV010000259         3044 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.264, whole genome shotgun sequence.
ACCESSION   DAPHCV010000259 DAPHCV010000000
VERSION     DAPHCV010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.264"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            246..773
                     /locus_tag="RH033_004747"
     CDS             246..773
                     /locus_tag="RH033_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDV2993802.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEVREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTVQMLGRAPQQNAQAQPKPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
     gene            831..1064
                     /locus_tag="RH033_004748"
     CDS             831..1064
                     /locus_tag="RH033_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HDV2993803.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRQDGTLIWRSWNFEDGAGYWMNQYIRDFGILK"
     gene            1123..>3044
                     /locus_tag="RH033_004749"
     CDS             1123..>3044
                     /locus_tag="RH033_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDV2993804.1"
                     /translation="MPVTKCEPETTRKASRKSVKTQETALSALLAQTEEVSVPLDSLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRYGVAAGGRRLAALNMLA
                     ERDIIQADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRILKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDGDGYVDRV
                     ALDAALLEKLQAVAEFLREAEGWGWCAGRMEPVGECREDARAYRNLPEPEAVLTEAED
                     ERLNELMARYDSLENQCEESDLLAAEMKLIDCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CIQRGVQLRSEDDVADDADRTEQGLESASVEEISLPLLTKLTSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFDSKAHSSPARIRLECEHYALTSDAPSGKEGAAFMALMAEHSRF
                     AALLPEGWERDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDSLETAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCSPRPQTDA"
BASE COUNT          689 a          801 c          976 g          578 t
ORIGIN      
        1 atctgtccag ggtgagatgc accggccaca aaatttttag ccatgaaccc ggcaaaaaat
       61 tttgcgtccg cccccggaca gctgccccgt gaggcggtaa acgggccccc atcggaaagg
      121 tccactgcgg ggagaaaccc ctgtgaagtg gacggcagga ggagctttcg ctcctctgaa
      181 ggggcacatg aacgtcccgt cgccggtatc cggtttatct cacatctttc agtcaggagg
      241 ttcttatgag tgcacgtggt atcaacaagg tcatcctcgt cgggcgtctg ggcaatgatc
      301 cggaagtccg ttacatcccc aacgggggcg cagtggcaaa cctgcaggtg gctacgtcag
      361 aaagctggcg cgacaaacag acgggggagg tgcgggagca gacggaatgg caccgcgtgg
      421 tgctgttcgg caagctcgcg gaagtggcag gtgaatatct gcgcaagggt gcgcaggtct
      481 acatcgaagg tcagcttcgc acccgtagct gggaagataa cggtatcacc cgttacgtca
      541 ctgaaattct tgttaagacc acgggcaccg tgcagatgct gggacgtgca ccacagcaga
      601 acgctcaggc gcaaccgaag cctcagcaga atgggcagcc acagagtgct gacgcgacga
      661 aaaaaggtgg cgcgaaaacg aaaggccgtg gacgtaaggc cgcgcagcca gagcctcagc
      721 cgcaaccgcc ggagggggag gattacgggt tttcagacga catcccgttc tgatcgggct
      781 gactgtgaca accgccccgc cctgtgcggg gcatcaccgg agatatgagg atgagcgaat
      841 atttcagaat acttcaggga ctgccggacg gctcctttac ccgcgaacag gcggaagccg
      901 ttgccgcaca gtaccggaac gtctttatcg aggatgatca gggaacgcat tttcgcctgg
      961 ttgtccgtca ggatggcacg ttgatctggc gctcctggaa ttttgaggac ggtgccgggt
     1021 actggatgaa ccagtacatc agggatttcg ggattcttaa gtaagagagg tgccggacgc
     1081 gcgaacgtcc ggcaggacat aagcaattat aaggggatga ttatgcctgt aacgaagtgt
     1141 gaaccagaaa ccacccgcaa agcaagccgt aaatctgtaa aaacgcagga aactgcactg
     1201 tctgccctgc tggcgcagac ggaggaagtg agcgtgccgc tggattcact gattaaatca
     1261 ccgctgaatg tgcgcacggt gccgtattct gcggagtccg tcagtgaact ggctgattcc
     1321 attaagggag tcggcctgct gcagaatctg gttgttcatg ccctgccagg tgaccgttac
     1381 ggtgtcgccg caggtggtcg ccgactggca gcactcaaca tgctggcaga gcgtgacatc
     1441 attcaggctg actggcctgt ccgtgtgaag gtcattccgc aggagctggc gactgccgca
     1501 tcgatgaccg agaacggtca tcgtcgggat atgcaccctg ccgaacagat tgccggattc
     1561 cgcgcaatgg cgcaggaagg caaaacgcca gcacaaatcg gtgatttgct gggctattca
     1621 ccccgccatg tccagcgaat actgaaactg gctgaccttg cgcctgtcat cctcgatgcg
     1681 ctggcagaag accgcatcac caccgaacac tgtcaggcgc tggcgctgga gaacgacacc
     1741 gcgcgtcagg tgcaggtgtt tgaagccgcc tgtcagtcgg gatggggcgg taaaccggaa
     1801 gtacagacca ttcgtcgtct ggtgaccgaa agtgaagtgg cggtggcggg gaacagtaaa
     1861 ttccgcttcg tgggggctga tgccttctcg ccagacgaac tgcgcaccga tttgttcagc
     1921 gatgacgggg acggttatgt cgaccgcgtg gcgctcgatg ctgccctgct ggaaaaactc
     1981 caggctgtcg cagagttcct tcgggaagcc gagggctggg gatggtgcgc cggacgcatg
     2041 gagcctgtcg gtgaatgccg tgaagatgcc cgggcatacc gcaacctgcc ggagccggaa
     2101 gcggtgctga cggaggcgga agacgaacgt ctgaacgaac tgatggctcg ttacgactcg
     2161 ctggaaaacc agtgtgagga atctgacctg ctggcagcag aaatgaagct gattgactgc
     2221 atggcgaagg tcagggcgtg gacgccggag atgcgtgccg gaagtggcgt ggtggtgtcc
     2281 tggcgttacg gtaatgtgtg tatccagcgt ggtgtgcagt tgcgcagtga ggatgatgtg
     2341 gctgacgacg ctgaccgcac ggaacagggg ctggagagcg cgtcagtgga ggaaattagt
     2401 ctgccgttgc tgactaaatt gacctcagag cgcacgctgg cagtccaggc agcactgatg
     2461 cagcaaccgg acaaatccct ggcactgctg gcatggacgc tctgcctgaa tgtgtttgac
     2521 agcaaagcgc atagtagtcc cgccagaatt cgcctggaat gtgagcatta tgcgctgacc
     2581 agcgatgcac cgtcagggaa ggaaggtgcc gcattcatgg cgctgatggc agaacactct
     2641 cgttttgctg ccctgctgcc ggaaggatgg gaacgggata tgacgacgtt cctgtcactt
     2701 agccaggagg tgctgttatc cctgctcagt ttctgtaccg cgtgcagtct taacggtgtc
     2761 cagacccgcg agtgtggtca cacgtcacgc agtccgctgg actcgctgga aaccgccatc
     2821 ggctttcaca tgcgcgactg gtggcagccg acaaaagcaa acttcttcgg acacctgaaa
     2881 aagccgcaga ttatcgcagc cctgaatgag gccggactgt ccggtgccgc acgggacgcg
     2941 gagaaaatga agaaaggcga tgccgctgaa catgcagagt tccatatgaa agacaaccgc
     3001 tgggtgcctg gctggatgtg ttcaccacgt ccacagacgg atgc
//
LOCUS       DAPHCV010000260         3007 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.265, whole genome shotgun sequence.
ACCESSION   DAPHCV010000260 DAPHCV010000000
VERSION     DAPHCV010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.265"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>3007
                     /locus_tag="RH033_004750"
     CDS             <1..>3007
                     /locus_tag="RH033_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDV2993805.1"
                     /translation="QEDARTFLDVAGEYLEMAGAIALAVNPVTGPVLMSRALHDQLST
                     EEGRSEMVDGVTHFVSELWQPTVASAAPGSTPTPDDKIDCHKHPSSLTQFLAQKKAAF
                     LDDPLGTALNLLSPDGMLETAFQGVNAVIGSISNLFKGDDEPPAAEYIAEGARDVRIN
                     SQPAARSGARCTCEARVVNEPGNGAFVSPDVRIGGPLLVVRDIRSGRSQITLVATVAL
                     MFLRPGKMLSKIACFAASVGMGVMTQKAISALPHPVNAATGAKYLADDDDFDFSLPGH
                     FPLDWQRVYSSRDDRTEGMFGQGWSVMYEVSLGRTPGTADENCMTFVSGMGRRLDMEA
                     VLPGSGFYSPGEGLAVRRGEQGHWLISSDDGQFFLFEADPHHPQRQRLKMLGDRNSNC
                     LNLYYDELGRITQISGEQQRPCIRLHYELAAHPRRVTQIYQHFPETAPLLLRRYRYDE
                     AGDLNGVYDSTGHLLREFAYDENHCMTLHRQPGGEGYYYQWSWYEGPDDAAWRVTGHH
                     TDSGEQYRLAWSLASRRLCVTDGLGRTRYHQWDAQNQVTAYQDEAGQVTTFRWSDEER
                     LLLGMTDAQGGKWRYVYDRQGHITETHDPLGRVAQTQWHPVWHQPETEVDAAGNSWCC
                     EYDERGNLLAVTDPLQQNTRYQYDRHGQVVQITDARGGNKYLQWNEDGQLMRHTDCSG
                     SQTAWFYDERTRLIRMTDAQSHSTRYGYDDSGHLTEVILADGRTVNYQSDAAGRLVKY
                     TSPMGRITRWQRDGQGRVRSRTDATGRRTAFGYDAYGRLVTLTNENGESYRFRHDVLD
                     RLAEQINPDGCRQTYRYNALNAVTEVVFTGDRGGEIRHRLARDAAGRLTAKETADSRT
                     EYVHDAADQLLEIRRRRSDAGETDAPEIIRFSYDRLGRMLTEETAQGVLTHQYDELSN
                     RTATTFPDGRTQRHLYYGSGHLQQINLDREVISEFTRDALHREVLRSQGRLSTRQLYD
                     PAGRLKRRETYSGMRGVVPETFTDRQYSY"
BASE COUNT          639 a          837 c         1040 g          491 t
ORIGIN      
        1 gtcaggagga tgccagaacg ttcctggatg tggccgggga atatctggaa atggcggggg
       61 ccatagccct ggcggttaac ccggtgacag ggcctgtgct gatgtcccgg gcgctgcacg
      121 accagctcag tacggaagaa gggcgttctg aaatggtgga tggcgtgaca cattttgtgt
      181 cggaactatg gcagcccacc gtggccagtg cggccccggg ctccaccccg acccctgacg
      241 ataaaatcga ctgccataaa catccctcct cactgacgca gtttctcgca caaaaaaaag
      301 ccgcctttct ggatgacccc ctgggaacgg cccttaacct gctgagtccg gacggtatgc
      361 tggaaaccgc ctttcaggga gtgaatgcgg ttattggcag catcagtaat ctgttcaaag
      421 gcgatgatga accgcccgca gcggaatata tcgcggaagg cgcgcgggat gtgcgtatca
      481 acagccagcc ggcggcacgc agcggggcac gctgcacctg cgaggccagg gtggtgaatg
      541 agccggggaa cggggcgttt gtgtcaccgg atgtccgcat tggcggtccg ttactggtgg
      601 tcagggatat cagaagcggc aggtcgcaga taaccctggt ggcaaccgtg gcgctgatgt
      661 ttcttcgccc gggcaaaatg ctttctaaaa tcgcctgttt tgctgccagt gtcgggatgg
      721 gggtgatgac gcagaaggcc atcagtgccc ttccgcatcc ggtcaatgcc gccaccggag
      781 ccaaatatct ggccgatgac gatgacttcg atttcagtct gccgggccac tttccgctgg
      841 actggcagcg cgtgtacagc agtcgtgacg accgcacgga agggatgttc gggcagggct
      901 ggagcgtgat gtatgaagtc agcctcgggc gcacgccggg tactgctgac gaaaactgca
      961 tgacctttgt gtcgggcatg gggcgacgcc tggatatgga ggccgtgctg ccggggagcg
     1021 ggttttacag ccccggcgaa ggactggcgg taaggcgggg tgagcagggg cactggctta
     1081 tcagcagcga tgacgggcag ttttttctgt ttgaagccga cccgcatcac ccgcagcgcc
     1141 agcggctgaa aatgctgggc gaccgcaaca gcaactgtct gaatctgtac tacgacgagc
     1201 tggggcgcat cacgcaaatc agcggtgaac agcagcgtcc gtgcatccgt ctgcattatg
     1261 aactggcggc gcatccccgg cgggtgacac agatttatca gcatttccct gagacggctc
     1321 ctttactgct gcgtcgttac cgctatgacg aggccggcga cctgaacggc gtgtacgaca
     1381 gcacgggaca cctgctgcgc gagtttgctt acgacgaaaa ccactgcatg accctgcacc
     1441 gccagccggg cggcgaaggg tattattacc agtggagctg gtatgaaggc ccggatgacg
     1501 cagcgtggcg ggtgacgggg caccacacgg acagcggtga acagtaccgg ctggcgtgga
     1561 gcctggcgtc acgcaggctg tgcgtgacgg acgggctggg gcgcacgcgt tatcatcagt
     1621 gggacgcgca gaatcaggtg acggcgtacc aggacgaagc cgggcaggtg acgacgttcc
     1681 gctggagcga cgaagaacgg ctgttactgg gaatgaccga tgcgcagggc ggcaaatggc
     1741 gctatgtgta tgaccggcag gggcatatca cggagacgca cgacccgctg gggcgggtgg
     1801 cacagacaca gtggcacccg gtgtggcacc agccggaaac ggaggtggac gcggcgggga
     1861 acagctggtg ctgtgagtac gatgaacgcg gcaatcttct tgcggtgacc gacccgctgc
     1921 agcagaacac ccggtatcag tatgaccgcc acggccaggt ggtgcagata accgatgcgc
     1981 gcggggggaa taaatacctt cagtggaacg aagacgggca gctcatgcgg cacacggact
     2041 gttccggctc gcagacggcc tggttctacg acgaacgcac gcggctgata cggatgacag
     2101 acgcgcagag ccacagcacg cgctacgggt atgacgacag cggccacctg acggaggtga
     2161 tactggcgga cggccgcacg gtgaattacc agtcggacgc ggcgggcagg ctggtgaagt
     2221 acaccagccc gatggggagg ataacgcgct ggcagcggga cgggcagggc cgggtgcgca
     2281 gccggacaga cgcgacgggg cgcaggacgg cgttcgggta cgatgcttac gggcgtctgg
     2341 tcacgctgac aaacgagaac ggagaaagct accggttccg ccatgacgtg ctggaccgtc
     2401 tggcggagca gataaatccg gacggctgcc gccagacgta ccgctacaac gcgctgaacg
     2461 cggtcacgga agtggtgttc accggggacc ggggcggtga aatccgccac cggctggcac
     2521 gtgatgcggc gggccggctc acggcaaagg agacagccga tagccgtacg gaatacgtcc
     2581 atgatgcggc agaccagtta ctggaaatcc gccgccgacg gtcggatgcc ggggaaacag
     2641 acgcaccgga gattatccgt ttcagttacg acagactggg gcggatgctg actgaggaga
     2701 cggcgcaggg cgtcctgacc catcagtacg atgaactcag caaccggaca gccaccacct
     2761 ttccggacgg cagaacgcag cgtcatctgt actacggcag cggccatctc cagcagataa
     2821 atctggaccg ggaggtcatc agcgagttca ctcgtgacgc cctgcaccgt gaagtgctca
     2881 gaagtcaggg gcgactgagc acgcggcagc tttacgaccc cgccgggcgg ctgaagcgca
     2941 gggagacata cagcgggatg cgcggggtgg taccggagac gttcacggac cggcagtaca
     3001 gctacag
//
LOCUS       DAPHCV010000261         2990 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.266, whole genome shotgun sequence.
ACCESSION   DAPHCV010000261 DAPHCV010000000
VERSION     DAPHCV010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.266"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(519..2133)
                     /locus_tag="RH033_004751"
                     /pseudo
     CDS             complement(519..2133)
                     /locus_tag="RH033_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2428..2650)
                     /locus_tag="RH033_004752"
                     /pseudo
     CDS             complement(2428..2650)
                     /locus_tag="RH033_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011846847.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          799 a          727 c          698 g          766 t
ORIGIN      
        1 gaccaaggcg gtagtgatta aagggggggt ggatataaaa atttattgta cgtggactgc
       61 accccaaaac ttggagaccc aactgattaa tgtgcaattt ttatggcaaa gccaaaatat
      121 tctctcgaaa ccagattggt tgtagttaat cattatcttt ccagacacga tggcgcacgt
      181 cgtatcgcta aacgttttgg tgttgaaaaa acatctgctg ggtaaggtaa gagactggta
      241 actccacgat attgatcgta tcacatggaa aaatgacctc cacactcctg agtttcgact
      301 gacggttgtc ctgaagtctc tgagtgaaga acttaccacg cgcgaggccg ctgcacggtt
      361 taatatctca aatgaatccg ttatccggca ctgggtgaag aaggtcttct gaacataaaa
      421 ccaggccgga gcaaagacat gacaaaatct caaaaaacac ctccacttac tgatgcagaa
      481 ctggaaaagt ggctcttctg caacctgggt ggagattctc atgccagtgg actcattacc
      541 ctctgtttga gtagttcaaa tccggctcga ccatacatct ggcgtttcaa catcttcagg
      601 cgatttacat gaccttcgac aacaccatta ctccagcgac tgctgattgc ctcacatata
      661 gccgcagcat cagcttccat ccccgcagcc acgcaccgaa gttctgctga gccgctttcg
      721 tggactcgag tgaacaagct gcttagctgt gatttatttt gggttttcag aatacggtag
      781 aactcgagta ccagttgctg cgctattttc agctccggtt ctttttcaca catcagacta
      841 ataaatcggg aagcataatt ttcttcaccc ctgattatta accagtggct tacccgggac
      901 actgaaggaa gtctgaagga agtctgacgg cagtagtaac cggtgggatc cagcctttac
      961 gcgatttggc aacagcatcc ctgacgatgg tttcactgcc tgtacccccc tggccaccat
     1021 ttcccgccat atcctgctgg cgttataatt accactttct cgttgctctt ttaaccactc
     1081 cctccatggg tccagaagcc ctcgctttgg aggccgggtt gacatttcag ggaatgttcc
     1141 tgaacgcccc ctgcgactga ctgtcacacg cgacaggcct gtaatacggg atatttccct
     1201 gaaactacat cccttgttat gcagggccat aacctctgtc caaagctgat gccgtttttt
     1261 gcgggtttgc tgtttaaggc gttccggacg acggagcgat actgcaggca cagatggttt
     1321 tgcccgggtt attcctttgg tgacaactaa ctggtaacaa gacgtatcag agtcatatgt
     1381 ctgtacatca ttcgttcagg cgcatcgcca atattgttta gcagaagcca tcgttcagct
     1441 acctgtctgg cctgaggtgc accttcacgt gctgctgtcg cattgattcc gcagcgatca
     1501 cgcgagacaa cctgtatttc cggatatttt ctgaaccagg tcgccagcgt atgctgatcg
     1561 cgaccgtgca acaggacgag gggacggtga gtatcaagat tgactattaa cataccgcag
     1621 cagtggcccc gatgccacgc cactcatcaa taccgacatg gggcgagcct gacagtttcg
     1681 tctccagggt attataagcc tgcgaagaag agtatctgca ctgcaacgga gacccactgt
     1741 cgttgctgcc cgttttccag cctcacctcc ggctattaat cccatctgac gctgtaaatt
     1801 ttgtaacgac tgtgaagact gctggtgtga actggctaag ggagcaagcg acccggcaaa
     1861 aattttacgt gaacaagccg ggttacggca gtaccagtgg cggacggaaa atacaagcca
     1921 tagcgtctgg cctgtgcagg gtagatgctg tatccggcgc cggggacaac tatgaacaga
     1981 atcgctacgc ttaaagcatt caggacattg cgctgttttg cgttttccgc ggagatggag
     2041 ggttataccg tcagatctaa taatttgtcg actgcattac catccgcaag gtaactgtag
     2101 aagtgtctta agagagtgca ttgcgttgcc cataactgaa aacagcttag atccttgttc
     2161 accagaaagt ttcatccatt ttcttccacc caggttgcgg aagagctact tttcatccgt
     2221 cgttgacaaa taatgatgag atagtcacag agcagcgttt gggaagcgca caatcacaat
     2281 attgtacggt tgcattatcg cggtagagta taccgcatgc taaaaataag ttaagtaagc
     2341 gtctgaatga tattacatac tatatgtatg gaaccaaaat acatttttga ttaattactt
     2401 gctttttcag gacggagacg tatatctcat cctggataca actccacaga gaggagaatt
     2461 gtttttatcc ggaacaaatc taggaccacg caggaaacag agtaataccg caaagttcta
     2521 gtgattagct ggacaccgct gcttattaat gagagcaata tttctgtaac aaagataaca
     2581 cattctgagt cagaataatt tcatcatcag gtataaccag caggcaagct tgcgaacgga
     2641 gcgtgcacac agcccagctt gcagcgaacg acctgtacca aaatgagata ccaacagcgt
     2701 gagctatgag aaagcgctac gcttcccgaa gggagaaagg cggagaggta cgcacaaggg
     2761 agcttccaga gggaaaggga gtaccagctg gcgaatttgc actccaacaa ataattcccc
     2821 cttttctact ccactacttt tgaccatacg tctggcgctt aattcgttga ttgttggcct
     2881 tgcaagcgcc gttagacctg attcaccata cccaggcagt cttggaaacg gaaagcttgc
     2941 gcgttaagcg cacgttttaa ctaattcaaa tacataatga attgattcag
//
LOCUS       DAPHCV010000262         2956 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.267, whole genome shotgun sequence.
ACCESSION   DAPHCV010000262 DAPHCV010000000
VERSION     DAPHCV010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.267"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..299
                     /locus_tag="RH033_004753"
     CDS             <1..299
                     /locus_tag="RH033_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HDV2993806.1"
                     /translation="TDKAPAYGRALALLKREGRCPSDVEHRQIKYRNNVIECDHGKLK
                     RIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPLGEMRLVSRVFEM"
     gene            352..2924
                     /locus_tag="RH033_004754"
                     /pseudo
     CDS             352..2924
                     /locus_tag="RH033_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element ISEc63 family transposase"
BASE COUNT          783 a          650 c          790 g          733 t
ORIGIN      
        1 acacggataa agcgcccgcc tatggtcgcg cgcttgctct gctcaaacgc gaaggccggt
       61 gcccgtctga cgttgaacac cgacagatta agtaccggaa caacgtgatt gaatgcgatc
      121 atggcaaact gaaacggata atcggcgcca cgctgggatt taaatccatg aagacggctt
      181 acgccaccat caaaggtatt gaggtgatgc gtgcactacg caaaggccag gcctcagcat
      241 tttattatgg tgatcccctg ggcgaaatgc gcctggtaag cagagttttt gaaatgtaag
      301 gcctttgaat aagacaaaag gctgcctcat cgctaacttt gcaacagtgc ctttgatttt
      361 gccactgcat ggttacttca aaataaggta ttactgcccg gagcaaccac actagtacgt
      421 ctcatcagtg aaattcgtga aagggcaaat cagcggctgt ggaaaaagct ggccgcactg
      481 ccgaacaaat ggcaggcagc tcaagtgatg gagcttctgg tcattccgga aggtcagcgt
      541 gtatcaccac tggaacagtt gaaaaaaggc cctgttacag tcagtggacc tgcgtttact
      601 gaggcgctgg aacgatatat ccgattacga agccttgagt tttcccgact gaacttttcc
      661 ggtctgcctg ccattcaact gcgtaatctg gctcgttatg ctggcatggc gtcggtaaaa
      721 tatatcgctc gaatgccaca gcagagaaag cttgctgtac ttactgcatt cgttaaagcg
      781 caggaaataa cggcattgga cgatgccgtt gatgtgcttg atatgctaat tctggacatt
      841 atccgcgaag caaagaaaac tgggaaaaaa aaagactcag gacgctgaaa gatcttgatc
      901 aggccgcatt gttactggcg cgggcatgtg cattgttgct ggatgataat acagatgttc
      961 cagatctcag gcaggttatc ttcaagtgcg tacccaaaaa cagactggca gaatctgtaa
     1021 gtaaggttaa tgaacttgct cgtccacata acaataattt ccatgacgaa atggttgagc
     1081 agtacggtcg ggttaaacgt tttcttccgg cggtgttgcg gaacctgcat ttccgtgcgg
     1141 caccggcagg tgaacatgta ctggctgcga ttcattatct ggcagaactg aatggttcga
     1201 aaaagcgcat ccttgatgat gcgcctgaac atattatcac cggtccctgg aaacgcctcg
     1261 tatacgatgc ggagggacgg atacagcgtg caggttattc actatgtttg ctggaacgcc
     1321 ttcaggatgc actgcgccgc cgggacatct ggcttgaaag cagtgatcgc tggggagatc
     1381 ctcgcgagaa gttgttgcaa ggtgaagagt ggcagactca gcgtattcct gtctgtcggg
     1441 cactgggaca tcctgtcgat ggacgtaaag gtgtgcaaca actggctatt cagctggatg
     1501 agacctggaa agccgtggca tcacgatttg aaaagaatgc agaagttcat atctgtaatg
     1561 aaggtaaata tccatccctg actatcagtt gtctggagaa acaggaagag ccaccatcgt
     1621 tgcttcgtct aaataatcgg atcaaacagc tactcccacc ggtagattta acggaactgt
     1681 tacttgagat agatgcccgg acaggattta cacatgagtt tgcacatgtc agtgaatctg
     1741 gtgctcgagc gcaagatttg cacatcagtt tatgtgcggt attgatggct gaagcctgta
     1801 atatcggact ggagccgctg ataaagcaca atataccagc actgacccgt catcggctca
     1861 gttgggtgaa acagaattac cttcgtgcag aaacgctagt cagcgccaat acccgcctgg
     1921 ttgattttca gtccacactg gagcttgctg gtcgttgggg aggtggagaa gtggcatcag
     1981 ctgacggcat gcgttttgtc acaccagtga agactatcaa ctcaggatct aacagaaaat
     2041 attttggttc tggacgaggc atcacctggt ataacttcgt atctgatcag tactctggtt
     2101 ttcatggcat tgtggtaccc ggtacattac gggattcgat ttttgtactg gaaggacttc
     2161 ttgagcagca gacagggctg aatccagttg agatcatgac agacactgcg ggtagcagcg
     2221 atattatttt cggtctgttc tggctactgg ggtatcagtt ttccccccgg cttgccgatg
     2281 caggtgaagc ggtgttctgg cgggtgaata aatcggcaaa ctacggagta ctggataagt
     2341 tggcccgagg gtatgcagat ctgtcaaaag cggagagtca gtgggatgag atgatgcgaa
     2401 ccgctggttc gctgaagttg ggtacaattc atgcgtcaga acttattcgc tctttactga
     2461 aaagctcgcg cccatcagga ctggcacagg caattatgga agtagggcgt gtaaacaaaa
     2521 cgctatatct cctcaattat attgatgatg aagattatcg tcggcggatc ctgacgcagc
     2581 tcaatcgagg agaaggccgc cacgctgtgg ctcgtgcgat ttgttacgga caacgcggag
     2641 agatcagaaa gcgttatcgt gaagggcagg aagatcaact gggggctctg ggcctggtta
     2701 ctaatgcagt ggttctgtgg aacacgctct atatgcagga agtgttgagc tggatgcgcc
     2761 gtaatggaga agaaattata gatgaagata tcgctcggct atctcccctg atgcacgggc
     2821 atatcaatat gttgggccat tatacattca cactgccaga ggatatttta aaaggagagc
     2881 tgagagctct aaatttaaat ataaacaacg aattatctcc ttaacgtacg ttttcgttcc
     2941 attggccctc aaaccc
//
LOCUS       DAPHCV010000263         2952 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.268, whole genome shotgun sequence.
ACCESSION   DAPHCV010000263 DAPHCV010000000
VERSION     DAPHCV010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.268"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            452..886
                     /locus_tag="RH033_004755"
     CDS             452..886
                     /locus_tag="RH033_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HDV2993807.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFREAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPE"
     gene            979..1245
                     /locus_tag="RH033_004756"
     CDS             979..1245
                     /locus_tag="RH033_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993808.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAPDEIN
                     PDVLAMGGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            1422..1811
                     /locus_tag="RH033_004757"
     CDS             1422..1811
                     /locus_tag="RH033_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HDV2993809.1"
                     /translation="MKSCFDTDKAFHMSFDEVTFNADRIRSKLELLSEELMPRCGLVY
                     ELYQRRLSAAIDNFVATLPTEQHLLVLELAREEFDYLSAEEIAEEIRRDSERGYCCHG
                     FERNCCPLGCGDLGDDCCDAADLMEEP"
     gene            1808..2710
                     /locus_tag="RH033_004758"
     CDS             1808..2710
                     /locus_tag="RH033_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDV2993810.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPKVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGRMEGALEKALAIACQLQK
                     MGMTPEQIKQATGLSDDELKKITH"
     gene            2710..2943
                     /locus_tag="RH033_004759"
     CDS             2710..2943
                     /locus_tag="RH033_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993811.1"
                     /translation="MRTAKPGKIPGFFACRSRANSMTIAERLIQKGFEKGALEVAREI
                     ACRLRDMGWTPERIQEATGLSGEELKKLFPDEQ"
BASE COUNT          689 a          779 c          842 g          642 t
ORIGIN      
        1 tttcgggctg ccgctacggt ccgatgtctg aaaaaccagg cttgcagcga ccgttccggt
       61 cgcgccgcct ctgtcgcgcc gtgacctgcg ctccctgcac acctgacggt gtgtaaggaa
      121 actgcgcctg atggcgcagt tgtctgccct tccgccgccc tccctgcggc ctgtttcccc
      181 gctgtggact ttccgggggc tgaagcggcc acaacacgtt tcagccgtcc aggggagaga
      241 tgtaccagcc acaaaacttt ttgccgcaag cgtcaaaaac ttttgcgtct gcccccggac
      301 aactgccccg tgccgtggca gtcgctgccc catcggaaag gtccactgcg gggagaaacc
      361 ccgaagaagt ggacggcaga aggggctttc gcccctccgg gggagcaacc ggtcggcccg
      421 gcgacctgat tcagcagaag gagacgtttt catgacagtc agcagcacga tttccgtttt
      481 ttgccgcgac ggggtgttcc gtaccgttta ctgccacctg cacggtgagc cgacctggaa
      541 cggtcgcatc ctgcataccc actatgccac cggtcagcag gccgaagccc tggttgaaca
      601 cggtgatatc cgttgcctcg gtccccgttg cgacaaaccc gccgggcata cgcttcagaa
      661 cccggtggac ggtgtgacgg cttattacgg acgtgacagt ggtttccgga tggacagtga
      721 ggcgcgtgag taccgttctt tcagggaggc catcgccacg gaaagcactg aagaggtgcg
      781 cttccattat gtgttcatcg acggctactg gaaggtgatg taccgcacgc cggaaggctg
      841 gaagatgaaa gcgctcgcgc tggcactgcg tcgctgtccg gaatgaaaaa aagcagggcg
      901 ataaaccctg cctttctccg ctggcgcttc cccgtacgga agtccggcat ctcattcact
      961 aactatggag attatgccat gagaccatca attatcttcg caaccgccga gtatgtaaag
     1021 cgtctgcgag aagagtgcct gcgggagaac aaacccctgc accgccatac ccgcttcaga
     1081 cgccaggaac tggcaccgga tgagattaac ccggacgtcc tggcgatggg cggtcatatc
     1141 gccagacgct gcagtgagca gaagcgggta cgtatccccg ccatgaaagt cagcgaatgg
     1201 ggccacctgc tccgcgcgct tgaaattgaa cgggtctgtc actgattaac ccctgccgtc
     1261 tgcctgtgca ggtggcagga tgtgttcagg tactttacca ggaggaaccg tgagcaagat
     1321 gaaaaacatc acgacgtctg tgttgcatgg tgtctccaca aaaaacagaa aagggaaaca
     1381 gattctcttt cattcagggc ccgggagggc tggagataac aatgaagtct tgttttgata
     1441 ctgacaaagc ttttcatatg tcttttgatg aagtcacttt caacgctgac agaattcgct
     1501 caaaactgga actattatcc gaagaactaa tgccccgttg tggtcttgtt tatgagctat
     1561 atcagcgacg tttgagtgct gctattgata attttgtggc cacattgccg acagaacaac
     1621 atctactggt cctggaactg gcccgtgagg agtttgatta cctttccgca gaagagattg
     1681 ctgaagaaat acgccgtgac tcagaacgcg gctattgttg tcatggcttt gaacgcaatt
     1741 gctgtcctct cggttgtggg gatttgggcg atgattgctg cgatgctgct gatttgatgg
     1801 aggaaccgtg agtaagaaga aaaacaccac aacgcccacg ccgcatgatg ccgcgttccg
     1861 gtcgttcctg gcgaatcccg acgtcgccag agattttctg gaactgcatc ttcctgcgga
     1921 gtaccggcag ttgtgcgacc tgtccacgct gaagctggaa cccgccacct ttgtagagcc
     1981 ggacctgcat cagtacgcca gcgatatcct ctggagcgtg aaaaccaccg ggggtgaaga
     2041 cggctatgtt tatacgctca tcgagcacca gagcacagaa aatctgtaca tgccttttcg
     2101 catgttacgc tacagtgtgg cggcgatgca gagacatctg gagcagcaca aaacgttgcc
     2161 actggtgatt ccggtgctgt tctatcacgg tgagcgcagc ccgtacccgt acagcatgaa
     2221 ctggctggac tgttttgaga atccggcact tgcggctaaa atatacacaa agccgtttcc
     2281 gctggttgat atcactgtcg ttgatgacaa tgaaatcatg aatcatcgcc ggatggccgc
     2341 actgacgctg ctgatgaagc atatccgcca tcgtgacatg atggagctgc tggacaaact
     2401 cccgaaggtc atggtggaaa tttcagacga gcaggtgcgt gttctgattc attacatcgt
     2461 taacgcaggg gactctgtat caccggaatt tatgcgggcg ctggctgagc gtctgccgca
     2521 gcatgaggat aaactgatga ctatcgcaga acgtcttgag caaaaaggtc gccaggaggg
     2581 caggatggaa ggtgcgcttg aaaaagccct ggctattgcg tgccagcttc agaaaatggg
     2641 gatgacgccg gagcagatta agcaggctac cggactttcc gatgacgaac tgaagaaaat
     2701 cactcactga tgagaaccgc aaagcccggc aaaatcccgg gcttttttgc ctgcaggagc
     2761 agagcaaaca gcatgacgat tgcagaacgt cttatacaga aaggttttga aaaaggtgct
     2821 cttgaagtgg cgcgggaaat agcctgccgg ctgcgggata tgggctggac gccggaacgg
     2881 attcaggagg cgaccggact ttccggtgaa gaactgaaaa agctgtttcc tgatgagcag
     2941 tagcctggca tt
//
LOCUS       DAPHCV010000264         2929 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.269, whole genome shotgun sequence.
ACCESSION   DAPHCV010000264 DAPHCV010000000
VERSION     DAPHCV010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.269"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..2204
                     /locus_tag="RH033_004760"
     CDS             <1..2204
                     /locus_tag="RH033_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pcar"
                     /protein_id="HDV2993812.1"
                     /translation="KTAQVMKEEYQERIAIIQKKLNNDVSRLEENIKKNRCEIQNASS
                     YDLAFFINKMGRSGFERYIACCSTPEQHDGESITDNAANIDFIYFLLSHGYLSTDYMA
                     YRSVFMPGSLSTEDNNFIRAVTSGRLPDETAKMPLSNIANTVAKLHGLGILMHDNAWH
                     PQILWYLMRNDTNSLKTIMRMQAEVGAERRMVRLANEIFPLWEPAAQREYIRLMVDGD
                     GHLSTMIHQIGRLNDTVAEQNLLPVLLSLPILSWEAVSQITREELQRLIDLQFNLVTS
                     LPENCAQFFCENLRNSGCRLTNIPLARSDSGQETLHLVVQKKLWTYSTLNLQNICFSL
                     SHESENNSDTFRKKPVALIKSLRIPNLEKYVYENISSFIRDVFIHSEENDLIPDFLNS
                     TFVDWDDAKYMTESMSFVLEDVSVILNKENTETTEISYDQNLYSLLAHHNHITPCWNN
                     VISLLSEDASIAGDTFCEWLNINYSLLPNDSLPLTDVQFSQLLIKAVTSPHISKEALI
                     AITMAFRITLINVPENLPLNNAAVLIKQKWLAPTSTVFEQLYQALYEEGDKLTSLLYA
                     LICARPVLLSDNYELVLFSDDQFDLGITRLILNGDKIADEVCISILNWLWEKDEALLS
                     EAPLLSQQALIRFSTKITDDRQKQALLMQCLKNDGGSHKFIRQVLMTFGHQDYAAFLT
                     ERNYRSIPRSDAMWQLAVQLGNSGFIRPPKLTHADTRIRIEPFFNAENEYD"
     gene            complement(2249..2665)
                     /locus_tag="RH033_004761"
     CDS             complement(2249..2665)
                     /locus_tag="RH033_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HDV2993813.1"
                     /translation="MNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITY
                     SEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRAAVDATTEVKVALRLAGTPIGPN
                     DTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG"
     gene            complement(2662..2892)
                     /locus_tag="RH033_004762"
     CDS             complement(2662..2892)
                     /locus_tag="RH033_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDV2993814.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLR
                     PSWSSFLELEKADPGFMTEREDVVCDEGRFNL"
BASE COUNT          793 a          676 c          663 g          797 t
ORIGIN      
        1 aaaaaacagc tcaggttatg aaggaggaat atcaggaaag aatagcgatt attcaaaaga
       61 aactaaataa tgatgtttct cgattggagg aaaatattaa aaaaaacagg tgcgagattc
      121 agaatgcatc ctcgtatgat cttgcttttt tcatcaataa aatggggaga tcaggtttcg
      181 aaagatatat tgcatgttgt tcaacgcctg aacaacatga cggagaatcc attactgata
      241 atgccgcaaa tattgatttt atttatttcc ttctatctca tggttatctt tccactgact
      301 atatggccta ccgctcagtc tttatgccag gtagtctgag cacggaagac aataacttta
      361 tcagggctgt gacgtcagga agattacctg atgaaacggc aaaaatgccc ctcagcaata
      421 ttgctaacac agtagcaaaa ttgcatggac tgggcatact gatgcatgac aatgcatggc
      481 atcctcagat tctctggtac ctcatgcgca atgatacgaa ctctctgaag actatcatga
      541 ggatgcaggc agaggtcgga gccgaacggc ggatggtaag actggccaat gagatcttcc
      601 ccctatggga acctgctgcg cagcgggaat atatcagatt aatggtcgac ggggatggtc
      661 atttatccac tatgatccac cagattgggc gcctgaatga taccgtagct gaacaaaatc
      721 ttctcccggt attactgtcc ttaccgatac tttcttggga agccgtctct cagataactc
      781 gggaagaact gcagagactc atcgatttgc aatttaatct cgtaacgtcc ttaccggaga
      841 attgtgcgca gtttttctgt gaaaacctgc ggaattcagg ttgtcgcctg actaacattc
      901 ctttggcccg gagtgactct gggcaggaaa cattgcattt ggtggtacag aaaaaattgt
      961 ggacctattc cacgttaaac ctgcaaaaca tttgtttctc actttctcat gagtcagaga
     1021 ataacagtga taccttccgt aagaagcctg ttgctttaat aaaatcgctt cgtattccga
     1081 accttgaaaa atatgtttat gaaaacatat catcattcat tcgtgatgtt tttatacact
     1141 ctgaagaaaa tgatctcatt cctgattttc ttaattcgac attcgttgac tgggatgatg
     1201 caaagtacat gacagaaagt atgagcttcg tactggaaga cgtatcagtt atcctgaaca
     1261 aagagaatac cgaaacaaca gaaatttctt atgatcaaaa cctgtattca cttctggctc
     1321 atcataatca tattacccct tgctggaata atgttatttc tctactgagt gaagatgcgt
     1381 caattgctgg tgatactttt tgtgagtggc ttaatattaa ttattcttta ttgccaaatg
     1441 actcgttgcc tttgactgat gttcagttct cgcaattact tattaaggct gttacatcac
     1501 ctcatatcag taaagaagcg ttaatagcga taaccatggc atttaggata actcttatta
     1561 atgtgccaga aaacttacca ctgaataatg ctgcggtact aatcaagcaa aaatggctgg
     1621 caccaaccag tactgtgttt gaacaacttt atcaggcact gtacgaagaa ggtgacaaac
     1681 tgacatcact actttatgca cttatttgtg cccgtcctgt attattgagt gataattacg
     1741 agcttgtttt attttctgat gaccagtttg acctggggat tactcgttta attctgaatg
     1801 gcgataagat tgcagatgaa gtctgtatca gtatcctgaa ctggctctgg gaaaaagatg
     1861 aagccctgct ctctgaggca ccacttctca gccagcaagc cctgataagg ttctccacca
     1921 aaataaccga tgaccggcag aagcaggcac tgctgatgca atgtcttaaa aatgacggtg
     1981 ggtcacacaa gttcatcaga caggtgctca tgacgttcgg gcatcaggat tatgccgctt
     2041 tcctcacaga gaggaattat cgtagcattc cccgtagtga tgccatgtgg caactggctg
     2101 tccagctggg aaatagtgga tttatcaggc cgccgaaact tactcacgct gatacacgta
     2161 ttcgtataga gccctttttt aacgctgaaa atgaatacga ctgacatcgc aaaatagtgg
     2221 taagtccgtc gggggcggcc ttactccttt atcctgccca gtcttccagt accagtccag
     2281 gcacccgctc aaactctctc acattattcg tcaccagtat tgccccggtg gcgatggcgt
     2341 gcccggcgat cgccgtgtcg tttggaccga tcggcgtgcc ggcgaggcgc agcgccacct
     2401 taacctccgt ggttgcatcc accgcagcgc gatcccaggg caggatggca tcgagacggg
     2461 cgcagaacgc gtcaaccagc tgcacgtggc gtggcgatgc ctttggaccg gtggcgccga
     2521 agcgcatctc ggagtaggtg atggccgaga ccacgatacg gtgaccgcgc agcaccgcct
     2581 gctccagatt cttaagcact gcttcaggct gctcgcgcat gatgaacgag cagatgttcg
     2641 tgtcgagcat atagatcttg ttcacaggtt aaatcgtcct tcgtcgcaga caacgtcctc
     2701 gcgttccgtc ataaagcccg gatcggcctt ttctagctcc aggaatgagc tccagctcgg
     2761 gcggagggga cgcagaatga ttctgtcccc ttcccgtaca atctccagct cactcacccc
     2821 ctcaaaatcc agatcgcggg gcagacggat cgcgcggtta ttgccatttt tgaaaataga
     2881 tacggttctc atgtcttcct cctggcaagg cggcttttat atatgttta
//
LOCUS       DAPHCV010000265         2901 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.270, whole genome shotgun sequence.
ACCESSION   DAPHCV010000265 DAPHCV010000000
VERSION     DAPHCV010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.270"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1165)
                     /locus_tag="RH033_004763"
     CDS             complement(<1..1165)
                     /locus_tag="RH033_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HDV2993815.1"
                     /translation="MDVFNTPISRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPE
                     AIIRALRKRLEHPILGYSRWQHDDYLNAIVNWYHQQYKTEIKPEWITYSPSVMYSIAK
                     AIELLTSHGDNILVFTPVYNAFFDIIKHSERNILTASLIIKNEGGYAINWQDFDLKIQ
                     SAKMVLLCNPHNPTGTVWSEEDLHKIAASCARHNVWLCSDEIHSDFVFNRSFTSALKI
                     KKEKVVVFNSISKTFNVPALTGSYMISTDDNFNHKFRTISRYRDFVNSPSVLNIIATI
                     IAYNECEGWLKSLKAHIASNIQFTQQYLNENTPELIVRPADGCYFSWIDCSAIGYSFD
                     EFYSRLIHEGKVGIMAGNVYGTEGEGYLRLNLACGREKLYMGLTRLASVIKNIN"
     gene            complement(1165..2745)
                     /locus_tag="RH033_004764"
     CDS             complement(1165..2745)
                     /locus_tag="RH033_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDV2993816.1"
                     /translation="MKQKKAWSFFQSLGKAFMYPIALLSVCGMMLGLGSGLASDDMAK
                     LIPLLAIPVIKTILDFIVSLGLFAFVNLPVLFAIAIPLGLLKEKEDKAYGAFSGLIGF
                     MAMHLGTNFYLKQHDLLVAADQMSTHGQTIILGIQSYNTSVLGGIIAGLLVASMYKKI
                     VNLRIPESLGFYSGPRLVPIITLIVMSGFGLIIPFIWPPFFNLFMLIGHWISTSGPVG
                     YFFYAVAERVTIPFGLNHLVTSVFRFTPIGGSAVIGGEEYYGTLNMFMAYVKENAVIP
                     LDLAGKMEQGKLMIQYGLAGAALAMYRTAHAQNRKAIKALLISGVLTVIIGGVSEPIE
                     FLFLFVSPLLFVFHAFMNGFANMVLPYMGVKMGFTGDLIQFISFGVLRGTRTGWPIAM
                     CVEVAYFFIYYFVFRWTILKFNLMTVGREESSPVTLNAHEDTAIADIPTLDKSELQAA
                     EQMVKALGGKENIKSLDNCVTRLRLTIADMGLIDEAAIKRAGGIAVVKLDQNTLQVII
                     GTKVIALRRDMDNYMGIC"
BASE COUNT          901 a          650 c          533 g          817 t
ORIGIN      
        1 gattaatatt tttaattaca ctcgcaagac gcgtaagccc catatataat ttttctcgac
       61 cgcaggccag gttcagacga aggtagccct ccccctccgt tccatagaca ttccccgcca
      121 taatgcccac tttgccttca tgaattaatc gactgtagaa ttcatcaaag gaataaccaa
      181 tagcagagca atctatccac gaaaaatagc aaccatcagc tggccttacg atcaattctg
      241 gagtattttc gttcagatac tgctgggtga attgaatgtt agaggcaata tgtgccttta
      301 atgactttaa ccatccctca cattcgttat aggcaataat agttgcaatt atattcagta
      361 cggatgggga gtttacaaaa tcgcgatatc ttgatatcgt tctgaactta tgattaaaat
      421 tatcgtcagt tgaaatcata tacgatcctg ttagcgcagg aacattaaat gtcttcgata
      481 tcgaattaaa caccactact ttttcttttt ttattttaag cgcagatgta aagctacgat
      541 taaaaacaaa atcactgtga atttcatctg aacataacca gacattatgt cttgcacagc
      601 ttgcagcgat tttatgcaga tcttcttccg accagacggt tcctgtcggg ttatgaggat
      661 tacataataa caccatcttt gcgctttgaa tcttaagatc aaaatcttgc caattgatag
      721 cgtagccccc ctcatttttt ataatcaaag aggctgtcag aatatttctc tcactatgtt
      781 taattatatc aaaaaatgca ttatagacag gtgtaaaaac gagaatatta tcaccatgcg
      841 atgtcaataa ctctattgct ttagcaatgg agtacatcac gcttgggcta taggttatcc
      901 attctggttt gatttcagtt ttatattgtt gatgatacca attgactatc gcattcagat
      961 agtcatcatg ctgccaacgg ctgtacccta aaatcgggtg ttccagacgt tttcttaatg
     1021 ccctgatgat agcttccggt atcggaagat ccatatctga aatagagaat ggtaatacat
     1081 cttttacacc aaatctgtcc tcgcaaaagt cccattgggt acaatatgtt cctttgcggg
     1141 aaataggagt attaaaaaca tccatcagca tatccccatg tagttatcca tatcccgacg
     1201 caaggcgatg actttagtac cgataatgac ttgtagggta ttttgatcaa gtttaacaac
     1261 cgcaattccg ccagctcttt ttattgctgc ctcgtcaatc aatcccatat ctgcgattgt
     1321 taaacgtaaa cgagttacgc aattatccag ggatttaata ttctctttac caccaagtgc
     1381 cttaaccatc tgctccgctg cttgcagctc tgatttatca agcgttggga tatccgctat
     1441 agccgtatcc tcatgagcgt tcagcgtgac aggactggac tcttcacggc ctaccgtcat
     1501 caggttaaat ttgagaatag tccaacggaa cacaaagtaa taaataaaga aataggccac
     1561 ttcgacacac atcgctattg gccaacctgt tcttgtgcca cgcaatacgc caaagctaat
     1621 aaattgaatc agatcgccag taaatcccat cttcacgccc atatatggca agaccatgtt
     1681 ggcgaagcca ttcataaaag catggaagac aaatagcagt ggactgacaa ataagaacag
     1741 aaactcaatc ggttcgctga cgccgccaat aatgactgta agcaccccgg aaataagcaa
     1801 tgctttgata gcttttctgt tttgagcatg agcagtgcga tacatcgcca gcgcggcacc
     1861 agccagacca tactgaatca tcagtttgcc ctgttccatt ttccccgcca aatccagcgg
     1921 aatgaccgca ttctctttga cgtacgccat aaacatgttc agggtgccat aatactcttc
     1981 gccgccgatc actgccgatc cgccgatggg ggtaaagcgg aaaactgacg tcaccaggtg
     2041 atttaaacca aaaggtatcg tcacgcgttc ggcgactgca tagaaaaaat agccaacagg
     2101 accggaagtt gaaatccagt ggccaatgag catgaaaaga ttgaaaaatg gcggccagat
     2161 aaaagggatg atcagaccaa atccactcat cacaatcagt gtaataatag gcaccaggcg
     2221 tggaccacta taaaatccta atgattcagg aatacgtaag ttaacgatct ttttatacat
     2281 actggcgact aataatcctg ctataatccc acccaacaca ctggtattgt aggactggat
     2341 tcccagaata atggtttgcc catgtgtcga catttgatca gcagcgacca ataagtcatg
     2401 ttgtttaaga taaaagttcg ttcccagatg catcgccatg aaaccaatta agccagaaaa
     2461 agcgccatag gctttatcct ctttctcttt taataacccc agaggtatag ctatcgcaaa
     2521 caatacaggt aaattgacaa aggcaaacaa accaaggcta acaataaaat caagaatggt
     2581 tttaattacg ggaatagcca gtagtggaat taattttgcc atatcatcgc tggctaaacc
     2641 acttcctagc cctaacatca tgccacatac acttagcaga gcgatgggat acataaatgc
     2701 ctttcccagg ctctgaaaaa aactccaggc tttcttttgt ttcatgcggg ttatctcata
     2761 taaatgttat atataattag tccattaata ctttggtgcg aatagagaga tatagttttt
     2821 cttccaaaat caattcatat ttaaaagtgg cgtacaaata cctttcaatt tcatgaattg
     2881 cgcgctgtaa cagaatgtct t
//
LOCUS       DAPHCV010000266         2759 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.271, whole genome shotgun sequence.
ACCESSION   DAPHCV010000266 DAPHCV010000000
VERSION     DAPHCV010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.271"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            44..2323
                     /gene="ydeP"
                     /locus_tag="RH033_004765"
     CDS             44..2323
                     /gene="ydeP"
                     /locus_tag="RH033_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HDV2993817.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETPLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWRTSSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2571..>2759
                     /gene="safA"
                     /locus_tag="RH033_004766"
     CDS             2571..>2759
                     /gene="safA"
                     /locus_tag="RH033_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HDV2993818.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSL"
BASE COUNT          736 a          643 c          677 g          703 t
ORIGIN      
        1 ctattacaaa tcctaataat tcatttccac acaggataag cagatgaaga aaaaaattga
       61 atcctaccag ggagctgccg gtggttgggg tgctgttaaa tccgtagcga atgcagtacg
      121 taagcagatg gatatacgcc aggatgttat tgccatgttt gacatgaata agccagaggg
      181 ctttgactgt ccgggttgtg catggccaga tcctaagcac agtgcgtcat tcgacatttg
      241 tgaaaacggc gcaaaagcaa tcgcctggga agtcacggat aagcaggtaa atgcctcttt
      301 ctttgctgag aatacggttc aatcattact tacctgggga gatcacgagc ttgaggctgc
      361 ggggcgtctc acccagcctt tgaaatatga tgccgtcagc gactgttaca agccattaag
      421 ctggcaacaa gctttcgacg aaattggcgc acgccttcaa agctatagtg atcccaatca
      481 ggttgaattc tatacttcgg gccgcacttc caatgaagct gcctttcttt atcagctttt
      541 tgcccgtgaa tacgggagca ataacttccc cgactgctcc aacatgtgcc atgaaccgac
      601 cagtgtgggt ttggcagcga gtatcggtgt aggtaaaggg accgtgttgc tggaagactt
      661 tgagaaatgc gatttagtca tttgcattgg gcataaccct ggtacaaacc accctcgcat
      721 gttgacttcg ttgcgcgctt tagtgaaacg gggagcgaaa atgatcgcca tcaatcctct
      781 acaggaacgt ggcctggagc gatttaccgc accgcaaaac ccgtttgaaa tgctgacgaa
      841 ctctgagact ccgttggcca gtgcctacta caacgtgcgc attggtggtg atatggcgtt
      901 gctcaagggg atgatgcgcc tgttaattga gcgcgatgat gctgcaagcg ccgcaggtcg
      961 gccctcattg ctggatgacg aatttattca aacgcatacc gtcggctttg acgagctacg
     1021 ccgtgacgtg ctcaattccg agtggaaaga tatcgaacgt atttctggat taagtcagac
     1081 tcaaatcgcc gaactggctg atgcatatgc cgctgccgaa cgaaccatta tctgttacgg
     1141 aatggggatc actcagcacg aacatggtac ccagaatgta cagcaactgg tcaatctgct
     1201 gttgatgaaa ggtaacattg gcaagcctgg tgcgggtatc tgcccactac gaggtcactc
     1261 taatgtacag ggcgaccgaa ccgtcggtat caccgagaaa ccgtctgcag agtttctggc
     1321 tcgcctgggt gagcgctatg gtttcacccc acctcatgca cctggacatg ctgcaattgc
     1381 cagcatgcaa gcaatatgta cggggcaggc tcgcgcattg atctgcatgg ggggcaactt
     1441 tgcactggca atgccagatc gggaagcgag cgctgtaccg ttaacgcaat tagatctggc
     1501 ggtacacgtt gccactaagc ttaaccgctc tcatctgctg accgcacggc atagctatat
     1561 tctgccggtt ctgggacgca gcgagattga catgcaaaaa agcggtgcgc aggcggtaac
     1621 cgttgaggat tcaatgtcga tgattcatgc ctcgcgtggc gtgttaaaac ccgccggtgt
     1681 aatgctgaaa tcagagtgtg cagtggttgc gggaatcgcg caggcagcac tcccccagag
     1741 cgtggtagcc tgggagtatc tggtggaaga ttatgatcgc attcgcaatg acattgaagc
     1801 tgtactgcca gagttcgccg actataacca gcgcatccga catcctggag gttttcacct
     1861 gataaacgca gctgctgaaa ggcgctggag gacgtcgtca ggtaaggcta atttcattac
     1921 cagtaaaggg ctgttagaag atccctcttc agcgtttaac agtaagctag tcatggcgac
     1981 agtacgcagt cacgatcagt acaacacgac gatttatggt atggatgatc gctatcgagg
     2041 ggtattcggt caacgagatg tggtctttat gagtgctaaa caagctaaaa tttgccgtgt
     2101 aaaaaacggc gaaagagtta atcttattgc gcttacgcca gacggtaagc gaagctcacg
     2161 ccgcatggat agattaaaag tggtcattta ccctatggct gaccgctcac tggtgaccta
     2221 ttttccagaa tcgaatcaca tgctaacact tgataaccac gatccattaa gtggcattcc
     2281 aggctataaa agtattccgg ttgaattaga accatcaaat taatgcctct tctcatttct
     2341 tctgctgtca tccgcacagc agaagaattc ctcattgact attatcgcgc aatttgctca
     2401 catggattaa attaaaccac atcctataag atataaactt ctgcctacag ctgtaagaaa
     2461 ctccgctcag tactgaagtg ccagtcctat ttcctctttt ctccagcctg ttatattaag
     2521 catactgatt aacgattttt aacgttatcc gctaaattaa catatttgaa atgcatgcga
     2581 ccacagtgaa aaacaaaatc acgcaaagag acaactataa agaaatcatg tctgtaattg
     2641 tggttgtctt attactgaca cttacgttga tagccatttt ttcggcaatt gatcagctgg
     2701 gtatttcaga aatgggtcgc attgcaagag atcttacaca tttcattatc aatagtttg
//
LOCUS       DAPHCV010000267         2750 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.272, whole genome shotgun sequence.
ACCESSION   DAPHCV010000267 DAPHCV010000000
VERSION     DAPHCV010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.272"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..934
                     /locus_tag="RH033_004767"
     CDS             <1..934
                     /locus_tag="RH033_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HDV2993819.1"
                     /translation="SITDVSNEITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAV
                     KLQQMQDGRLYIAGIGAGIENTPDGMQSQVLLAADRIAMVNPANGNTKPMFVGQGDQI
                     FMNDVFLKRLTAPTITSGGNPPAFSLTPDGRLTAKNADISGNVNANSGTLNNVTINEN
                     CQIKGKLSANQIEGDIVKTVGKAFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAV
                     AFSGAKHERENNDIYSSCRLIVRKNGAEIYNRTALDNTLIYSGVIDMPPGRGHMTLEF
                     SVSAWLVNDWYPTASISDLLVVVMKKATAGISIS"
     gene            1002..1601
                     /locus_tag="RH033_004768"
     CDS             1002..1601
                     /locus_tag="RH033_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HDV2993820.1"
                     /translation="MRKVCAAILSTAICLAVSGVSAWASEQQATLSAGYLHARTNAAG
                     SDDLNGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWYEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGNYLCVTDSKGKTHDVLTGSDDGRHSNTSLAW
                     GTGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            1666..>2750
                     /locus_tag="RH033_004769"
     CDS             1666..>2750
                     /locus_tag="RH033_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HDV2993821.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSKPGTLNDFLGAMTEDDVRPEA
                     LRRFELMVEEVARNASVVAQNTAAAKKSASDAGTSAREAATHATDAAGSARAASTLAG
                     QAATSAQEAFSSAGTASAKASEASKSAAAAESSKSAAATSAGAAKTSETNAAASQKSA
                     ATSASTATTKASEAATSARDASASKEAAKSSETSAASSASSAASSATAAGNSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSARAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQASAAARSASAAKTS"
BASE COUNT          687 a          688 c          822 g          553 t
ORIGIN      
        1 gtccattacg gatgtcagca atgaaatcac gcagaccgtc aataagaaac tggaagatca
       61 gagtgcggca atccagcaga tacagaaggt tcaggttgat acaaataata acctgaacag
      121 catgtgggct gtgaagctgc agcagatgca ggacggacgc ctttatatcg cgggtattgg
      181 tgccggtatt gagaataccc cggacggcat gcagagtcag gtgctgctgg cggcggacag
      241 gattgcgatg gttaatcctg cgaatggcaa cacaaaaccg atgtttgttg gtcagggcga
      301 tcagatattc atgaacgacg tgttcctgaa gcgcctgacg gcccccacca ttaccagcgg
      361 cggcaatcct ccggcatttt ccctgacacc ggacgggcgg ctgacggcga aaaatgccga
      421 tatcagcggt aatgtgaatg cgaactccgg gacgctcaac aacgtcacga ttaatgagaa
      481 ctgtcagatt aaggggaaac tgtcagctaa ccagattgaa ggcgatattg tcaaaacggt
      541 gggtaaggct ttcccccggg actcccgtgc accggagcgg tggccatcag gaaccattac
      601 cgtcagggtt tatgacgatc agccttttga caggcaaatt gttattccgg cggtggcatt
      661 cagcggcgct aaacatgagc gggagaataa cgatatttat tcgtcatgcc gcctgatagt
      721 acggaaaaac ggtgctgaaa tttataaccg taccgcgctg gataatacgc tgatttacag
      781 tggtgttatt gatatgcctc ctggtcgcgg ccacatgacg ctggagtttt cggtgtcagc
      841 atggctggta aatgactggt atcccacagc aagtatcagc gatttgctgg ttgtggtgat
      901 gaagaaagcc accgcaggca tcagtatcag ctgaatttta taacccatat acgggcacca
      961 gaaatggtgc cttttttatt gcagaaaagc gagaggtaat tatgcgtaaa gtttgtgcag
     1021 ccattttgtc cacagccatc tgtctggccg tatccggtgt gtctgcatgg gcgtctgagc
     1081 agcaggccac actgagcgca gggtatcttc atgcccggac gaacgctgcc ggtagtgatg
     1141 atctgaacgg gattaacgtg aaataccgtt atgagtttac ggacacgctg gggctgatta
     1201 cgtccttcag ttatgccaat gctgaagatg agcaaaaaac gcactacagc gatacccgct
     1261 ggtatgaaga ttccgtgcgt aaccgctggt tcagcgtgat ggcggggcca tctgtgcgtg
     1321 tgaatgaatg gttcagcgcg tatgcgatgg cgggtgtggc ttacagccgt gtgtcgactt
     1381 tctccgggaa ttatctctgc gtaactgaca gcaaggggaa aacgcacgat gtgctgaccg
     1441 gaagtgatga cggtcgccac agcaacacgt ctctggcgtg ggggactggc gtgcagttta
     1501 acccgaccga atccgtggcc attgatattg cttatgaagg ttccggcagt ggtgactggc
     1561 gcactgacgg tttcatcgtg ggtgtcggtt ataaattctg attagccagg taacacagtg
     1621 ttatgacagc ccgccggttc aggcgggctt ttttgtgggg tgaatatggc agtaaagatt
     1681 tcaggtgtac tgaaagacgg cacaggaaaa ccggtgcaga actgcacaat ccagctgaaa
     1741 gcaaaacgta acagtaccac ggtggtggtg aacacgctgg cctcagaaaa tccggatgaa
     1801 gccgggcgtt acagcatgga cgttgagtac ggtcagtaca gcgttattct gttggtggag
     1861 ggattcccgc cgtcacatgc cgggaccatc accgtgtatg aagattcaaa gccggggacg
     1921 ctgaatgatt ttctcggtgc catgacggag gatgatgtcc gtccggaggc actgcgccgt
     1981 tttgaactga tggtggaaga ggtggcgcgt aacgcgtccg tggtggcaca gaacacggca
     2041 gccgcgaaga agtcagccag cgatgccggc acatctgccc gtgaggcggc aacccatgcg
     2101 actgatgctg caggctcagc acgcgcagcc agcacgttag ccggacaggc cgcgacgtcg
     2161 gctcaggagg ctttttccag cgcgggaacg gcatcagcaa aagcctctga ggcatcaaaa
     2221 agtgctgccg ctgcagagtc ctcaaaaagc gcggcggcta ccagtgccgg tgcggcgaaa
     2281 acgtcagaaa cgaatgctgc agcgtcacaa aaatctgcag ccacttctgc atccaccgcg
     2341 accacgaaag cgtcagaagc tgccacctca gccagggatg cgtcggcttc aaaagaggcg
     2401 gcaaaatcat cagaaacgag cgcagcctcg agcgccagta gtgcagcttc ctcggcaacg
     2461 gcggcaggaa attccgcgaa ggcggcaaaa acgtccgaga cgaacgccag gtcttctgag
     2521 acggcagcgg gacagagcgc ctccgcagca gcaggctcaa aaacagcggc tgcgtcgtct
     2581 gccagagccg cgtcaacaag tgccgggcag gcctcagcca gtgccactgc cgccggaaaa
     2641 tcggcagaaa gtgctgcatc gtctgcttca acagccacaa cgaaggctgg cgaagccact
     2701 gaacaggcca gcgcagcagc gaggtctgct tccgcagcga agacatccga
//
LOCUS       DAPHCV010000268         2731 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.273, whole genome shotgun sequence.
ACCESSION   DAPHCV010000268 DAPHCV010000000
VERSION     DAPHCV010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.273"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..339
                     /locus_tag="RH033_004770"
     CDS             <1..339
                     /locus_tag="RH033_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV2993822.1"
                     /translation="RVNRGNLPAIKLGTARVRLARRGGKLQYRGSVLKVGKYLFRDAF
                     IQQLANGRWHVMRRIDGKNRYPIDVVKIPLSRPLTQAFEDARDRIIAAEMPKQLGYAL
                     KQQLRLWLTR"
     gene            336..737
                     /locus_tag="RH033_004771"
     CDS             336..737
                     /locus_tag="RH033_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV2993823.1"
                     /translation="MNRHTQIRQAVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDIPALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            749..1492
                     /locus_tag="RH033_004772"
     CDS             749..1492
                     /locus_tag="RH033_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV2993824.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGKGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVS
                     VTGLTVAPASASVKVGATTTLTFTVKPDGASDKAISVHSTDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTSDGDFVAVAAVAVSAAG"
     gene            1553..1939
                     /locus_tag="RH033_004773"
     CDS             1553..1939
                     /locus_tag="RH033_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HDV2993825.1"
                     /translation="MFLKQGTFNYEKQSVVLSELSGLQRIEYLAFVQQRTAKFDAEEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGAIENAGDLEHEVLTPEKS"
     gene            1948..2277
                     /locus_tag="RH033_004774"
     CDS             1948..2277
                     /locus_tag="RH033_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HDV2993826.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            2249..>2731
                     /locus_tag="RH033_004775"
     CDS             2249..>2731
                     /locus_tag="RH033_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDV2993827.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASVHAQTVEKSAQAHERMAREVEQTRQRMEALSQK
                     MREEQAQAMALAEAQDKAAAAFYRQIDSVKQASAGLQELQRIQQQIRQARNSGGIGQQ
                     D"
BASE COUNT          643 a          638 c          866 g          584 t
ORIGIN      
        1 cgcgttaacc ggggcaatct gcccgccatt aagctgggga cagcccgggt cagactggcc
       61 cggcgtggtg gaaaactgca gtaccgtggc agtgtgctga aggtgggtaa atatcttttc
      121 cgggatgcgt ttattcagca actggcgaat ggtcgctggc atgtgatgcg gcgtattgat
      181 ggcaaaaatc gttaccccat tgatgtggtg aaaatcccgc tttccagacc gctgacacag
      241 gcatttgaag atgcccgcga ccgcatcatt gctgcggaaa tgccgaaaca gctggggtat
      301 gcactgaaac aacaactgag gttatggctg acccgatgaa ccgacataca caaatccgcc
      361 aggccgtact ggcacgcctt cgggaacagt gtggagacag cgccacgttt tttgacgggc
      421 ttccggcatt tattgatgcg caggaactgc ctgccgtggc ggtgtggctg agtgatgctc
      481 agtacaccgg aaaaatgacg gatgaagatg actggcaggc tgttctgcat attgctgtct
      541 tcatccgggc acaggcaccg gattcagagc tggatatgtg gatggagagc accattttcc
      601 cggccctgaa tgatataccg gcactttccg gactcatcga caccctgatc cctctcggtt
      661 ttaactatca acgtgataat gagatggcca cctgggcgat ggcggaaatc acgtaccaga
      721 tcacgtacac gaattaaagg aggtggcaat gaccacacca aatccactgg caaaaacgaa
      781 aggtgcggga acgacgttct ggatgtacac cggcaagggc gatgcgtttg cgaacccttt
      841 atcggacact gactggctgc gtcttgcgat ggtgaaggat ctgcaacctg gcgaaatgac
      901 cgctgatgca gaagatgaca cttatctcga tgatgaagat gcagactgga aaacgacaac
      961 ccaggggcag aaatccgtcg gtgatacttc ggcgacgctg gcctggcgtc cgggtgacag
     1021 cgggcagaaa aaactggttc agttgttcga ctccggtgaa gtctgcgcgt ttcgtatcaa
     1081 atatcccaac ggcactgttg atgttttccg tggctggctg agctcactgg gtaaaaccat
     1141 tgcctcaaaa gacgtgatga cccgcacagt gaaaatcagc ggtgtggggc gtccgtatct
     1201 ggcagaggaa ggcactgaaa cagtgagcgt taccgggctg acggtggcac cggcatctgc
     1261 cagtgtaaaa gtgggagcaa ccaccacgct gacctttaca gtaaaacctg acggagccag
     1321 tgacaaagcg atcagtgtgc attcgacaga tccacagact gccacggtga ccctgaacgg
     1381 gcttgtggcc acggtgaaag gcgtgaagca gggcagtgtc agcattgtgg gcatgacttc
     1441 tgacggcgat tttgtggcag tggctgcggt ggctgtcagc gccgcaggtt aacaggacga
     1501 tactcatcat ttgccccggt tatccggggc ttttttgcag gtggagaaca tgatgtttct
     1561 gaaacagggc acgtttaatt atgaaaagca gtccgtggtg ctcagtgagc tgtccgggct
     1621 gcagagaatt gaatatctgg cgtttgttca gcagcgaacg gcaaagtttg atgccgaaga
     1681 gggagaactg ccggaggctg aacgacagat tgcttttctg cggatgggga tggatatcaa
     1741 tgcctggctg gtttcccgct cactgtggaa tgcggaacag tctcaggatg ttgagacgct
     1801 ttgcgcatcc gttattacaa catggtcgta tgatgccctg ggagcggggg cggagatggt
     1861 tctgtcgctg agcggtatgg gagccattga gaatgccggg gatttggagc atgaggtgct
     1921 gacgccggaa aagtcctgac gcgggaaatg cagtttgtca tgcggcttgc ccgggagttc
     1981 cggcgggcag actggcggcg gatgctgtcg gaaatgtcgg ccactgagct tggtgagtgg
     2041 ggcgattatt tccggatgca gagcttcagt gatgtgtgga tggatgcgca gtttgcctcg
     2101 ctgaaggcat tgatcgtgag aatggtgtcc ggtagcagtg atgctgcggt ggctgatttc
     2161 agccttttac cggaagagaa cgggataccg gagcgaacgg acgaagaact gatgcatctt
     2221 ggggaaggta tttccggagg tgtgcgttat ggaccagata gccaacctgg tcattgattt
     2281 ggggattgat gcggcagagt ttaaaaatga aattccccgt atcaaaaacc ttctgaatgg
     2341 tgcagccagc gatgcagaac ggtcttctgc ccgtatgcag cgttttatgg agcgtcagac
     2401 tcaggccgcc cggcagacaa tgcaggcggc ttcttcggct gcaacagccg catccgtcca
     2461 tgcgcagacg gtggagaaga gcgcacaggc tcatgaacgc atggcccgcg aggtggagca
     2521 aacccgccag cgtatggagg cactgagcca gaaaatgcgc gaggaacagg cgcaggccat
     2581 ggctctggcg gaggctcagg ataaagcggc tgccgcgttt tatcgtcaga ttgacagtgt
     2641 gaaacaggcc agtgcggggc tgcaggaatt acagcgtatt cagcagcaga tccgacaggc
     2701 cagaaacagt ggcgggattg gtcagcagga t
//
LOCUS       DAPHCV010000269         2688 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.274, whole genome shotgun sequence.
ACCESSION   DAPHCV010000269 DAPHCV010000000
VERSION     DAPHCV010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.274"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..104)
                     /locus_tag="RH033_004776"
     CDS             complement(<1..104)
                     /locus_tag="RH033_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HDV2993828.1"
                     /translation="MNRVTAIISALVICIVVCLSWGVNHYRDNAIAYK"
     gene            complement(101..598)
                     /gene="rrrD"
                     /locus_tag="RH033_004777"
     CDS             complement(101..598)
                     /gene="rrrD"
                     /locus_tag="RH033_004777"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HDV2993829.1"
                     /translation="MPPSLRKVVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKTLLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREIC
                     LWGQQ"
     gene            complement(598..813)
                     /gene="essD"
                     /locus_tag="RH033_004778"
     CDS             complement(598..813)
                     /gene="essD"
                     /locus_tag="RH033_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HDV2993830.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQLLDRVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            complement(1065..1439)
                     /locus_tag="RH033_004779"
     CDS             complement(1065..1439)
                     /locus_tag="RH033_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tolA family protein"
                     /protein_id="HDV2993831.1"
                     /translation="MLTFWVGFVAGCTPLHPSDCHKTTATGSCSSGRWDDQDEWGAQA
                     RGIRAAINAKLDEPHNWKGKKCRLHMEFSQDGTALKISTSNGDKAYCEAIKSAAHKAK
                     FPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(1611..2039)
                     /gene="tolA"
                     /locus_tag="RH033_004780"
     CDS             complement(1611..2039)
                     /gene="tolA"
                     /locus_tag="RH033_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HDV2993832.1"
                     /translation="MGFRSASILTLIISGIIIGCTDAVSTNYHDRTSYYSDKAIETQY
                     VSSSERTSDVSEDIRLYAHQIKSAIEKQFGDASKYSGKECTLRMHMAPNGLLLEVKRE
                     SGDLDLCREAMNAIKNADIPAPPSPEVYKVFQNGVLDFKP"
BASE COUNT          766 a          619 c          541 g          762 t
ORIGIN      
        1 tctttgtagg cgatggcgtt atcacggtaa tgattaacac cccatgacag gcagacgacg
       61 atgcagataa ccagagcgga gataatcgcg gttactctgt tcattgctga ccccacaaac
      121 agatttcacg ctcaatctca cgacgagtca tgagaccttt ccattgctta ccgccagcat
      181 atgtccagcg acgtagctga tcacatgcgc ctttgatatc gccctggttt attttgcgaa
      241 gaagcgtcga tgttctgaaa ttgccagcac ccacgttgta aacgaatgag taaagagcgc
      301 cgcgcgttgt ttccggtata tcgactttga tgtacgggtt aatttgtctg gcgaccgtgg
      361 caaggtcttt attcaagagt gttttgcatt ctgctttggt atacgtttta ccgagcatga
      421 tgtcttttcc tgtatgcccg tgacatacag tccatacacc aacaatatct ttgtatggta
      481 tgtagctgac accttccaga ccatcgttac cacttgggcc agtgattaac actgatgcta
      541 tagcaattgc tccgccacca atagcagcag caacgacttt tcgtaatgat ggaggcatta
      601 ttcacctctc gcagccttgc gcttatcttc tttaatcttg aaataaaggt ttgtcaggta
      661 cgtcagcagg ccaaatacca gactacccag cacaccgatt gcagcccact gtgacggagt
      721 tactctatcg agcaactgta aaaaccagta gccagcactg cctgcggagg tgccgtaggc
      781 aatgcctgtt gaaattttgt ccatggattt catagcctca cctccgcacg gaacggatgg
      841 catagttatt atgtgtaggc tttcagacac atcaatcaga gccttaattg atatatatgc
      901 tggagacgat gcaatataaa aagctcgccg tagcgagcta ataaaatgta tttctctgat
      961 attatgttta tttgtattag ctcagacttg acatcacagg tttcgtatat agaacatcat
     1021 caaatctgtc agtttgctat gaatgagata tagtaattga agagctaacc tcgcatgtca
     1081 aagccagatt tctgaaaatc tctgtagact tccggattgt tgaaggccgg aaatttggct
     1141 ttatgagctg cggactttat cgcttcgcaa taggctttat caccgttact ggtagatatt
     1201 tttaacgccg tgccatcctg agagaattcc atatgcaacc tgcatttttt ccctttccag
     1261 ttatgcggct catcaagttt ggcattaatt gcagctctga ttccccgcgc ttgcgccccc
     1321 cattcatcct gatcatccca gcgtcctgaa ctgcaactac ctgtagcagt agttttgtgg
     1381 caatctgaag ggtgtaaagg tgtgcatccc gcaacaaaac cgacccaaaa agtcaacata
     1441 acgattttct ttaatcccac ttcttgctcc tcaatccatt aaaatctcag caatagtagt
     1501 tgttacgtcc gccactggct cagagctgac tatccgctaa atttagctca gtgccgtagc
     1561 tgtgtcagaa caaacctaag ccgaaaccgt ttattacaaa acaataaata tcagggttta
     1621 aaatccagca ccccattttg aaatacttta tatacttccg gcgaaggggg ggcaggtata
     1681 tcagcattct ttatcgcatt catcgcttca cgacataaat cgaggtctcc actttctctt
     1741 ttaacctcca gtagaaggcc attcggggcc atatgcattc tcagtgtaca ctcttttcct
     1801 gaatacttac tcgcatcccc gaactgtttt tcgatggcgc tcttgatttg atgggcatac
     1861 agacggatat cctcactaac atcagaagta cgttcagatg aactcacata ctgtgtctct
     1921 attgctttat cggagtaata tgatgtacgg tcatgataat ttgtcgatac agcatcagtg
     1981 cacccgataa taatcccact aataatcaac gtaagaattg atgcgctacg aaaacccatt
     2041 tttcctcaca tatgtcatat agtaaaggat tatatatacc gttgttttgg acgctcaaac
     2101 agcgaatcag atcaaataaa acgcacattt gttaacattt acacaaagtc tgcgtgggat
     2161 attctgaaag aatatccata atgtggagag aatctattga agtgcatggt gccgggtgcc
     2221 tcccggtgaa caaaatgttc gtgatacctg tcggcgacag aaaaggttaa tggtatcacc
     2281 ccaccgcaca gggggattca ccatgcagga gttttcttag caaactcact gcgcgcccgg
     2341 caactcccaa ccacataaaa tgcggagttt gtggcattta tgcatataac tcgcaggaat
     2401 tatcttaaaa aactgatgtc gatccggatt aaaaagaagc aggtcatcat cagatgactg
     2461 gaaaaaagga aaacaaaaaa tactcatcat acagttttga ttgcagggat gagcctgcta
     2521 tgcacaatat gcagaatata agcaagataa aaatatgcag gcatattatt tcggattttg
     2581 ttattaacac aaccttttta ataatcattt ggcatacaat aaaccagccc agaaagaacc
     2641 gcctaaacag gcggttggtc aatacaaagg atgcttcgtc tttattat
//
LOCUS       DAPHCV010000270         2664 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.275, whole genome shotgun sequence.
ACCESSION   DAPHCV010000270 DAPHCV010000000
VERSION     DAPHCV010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.275"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            92..1366
                     /locus_tag="RH033_004781"
     CDS             92..1366
                     /locus_tag="RH033_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDV2993833.1"
                     /translation="MIPMKNYLFQTPEDICVQLKKMTHPVTIRTTDIANFWHYLESAT
                     LPVITKSTTTENREVTFLWRSEKALQGVYLRLNRVTDKKDVKKGLMTHIPSTDIWMLT
                     LVLPASYRGSYSFIEIPTDMTQKDIFQLGSRFSPLPGKSDPFNKTAEINIRGFGESVL
                     SLDMAPEQKEWDDTSHKCTGILSTLHSFVAGYQRRIRLYFPLNPTSVPLGLLVLPDAE
                     TWFDRIDITRALDMAITTGRITPMAIMGIDNINESDRMNILGGNKELIFDIAENLIPQ
                     LYRDYPDIVWAGRSNTVLAGQSLGGVTALMAAIYASTTFGTIISHSPSMWWNPDQGSP
                     ILFTENNTSWVSEQILSAPPKDVNIRLGVGSLEGTTVSHVQRLHHSLIAAGLESNLTV
                     YTGGHDYAWWRGAIIDELANYNCRKVSNNNSV"
     gene            complement(1508..2632)
                     /locus_tag="RH033_004782"
     CDS             complement(1508..2632)
                     /locus_tag="RH033_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDV2993834.1"
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANK
                     AAEAGLVVFDAAPSLDSEAGYRHQEELRKKSNIIGHFSFFSDEMADNLIDFAGKWRPD
                     LIVYPPLGPAGPLVAAKYRIPSVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLCD
                     LAWIDVAPPSMSILKNAEEPIISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKPM
                     VDGLELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADGF
                     IHHGGAGNTLTALYSGIPQIVFGEGADRSVNAEIVAKRGCGIIPDKHGLTSDLVNRLL
                     YDDSLRFCSDQVAAEMAEQPTPAEIAEVLMRKLKNNGKQL"
BASE COUNT          803 a          596 c          507 g          758 t
ORIGIN      
        1 caatacaaca gatttaattc cggaaagtca ctcatcatcc cttgaatgaa cgtcatttga
       61 tgtattattc ttacacttgg attaatttaa gatgatacct atgaaaaact atcttttcca
      121 gactcccgaa gatatttgtg tacagttaaa aaaaatgaca catcctgtca caataagaac
      181 aacagatatt gctaatttct ggcactatct tgagtcagca acacttccag tgatcacaaa
      241 aagcaccact acagaaaatc gggaggttac atttctgtgg cgctcagaga aagcactgca
      301 aggcgtatat cttcgcctga atcgtgttac agataaaaaa gacgtcaaaa aaggactaat
      361 gactcatatc ccttcgacag atatctggat gctgacactg gtgttaccag cttcatatcg
      421 gggctcatac tcatttatag aaattcccac agatatgaca caaaaagaca tatttcaact
      481 aggaagtcgc ttctctccat tacccggtaa atctgatcca tttaacaaaa cagcagaaat
      541 aaatatacga ggattcggag aatcagtcct ttctcttgat atggctcctg aacaaaagga
      601 atgggatgat acttcccata aatgtacagg tattctttca acattacatt cctttgttgc
      661 aggatatcaa cgccggattc gtttatattt ccccctgaat ccaacatcag tacctcttgg
      721 attacttgtg ttacctgatg ctgaaacatg gtttgaccgg atagatatta cccgggcatt
      781 agatatggcc atcaccactg gtcgtatcac gccaatggca attatgggga tagacaatat
      841 taatgaatct gatcgtatga atatactggg aggcaataaa gaactcatct ttgatatagc
      901 ggaaaatctg ataccccagt tatacagaga ctatccggat atcgtatggg ctggtcgctc
      961 taatactgta ctggccgggc agagcctcgg tggagtgaca gcactgatgg cagctatata
     1021 tgcgtcgaca acatttggta caatcattag tcactcacct tcaatgtggt ggaaccctga
     1081 ccagggcagc ccgatcttgt ttactgagaa taatacctcc tgggtaagtg agcagatact
     1141 ttcagcgcct ccgaaagatg taaatatccg acttggagtc ggttctttag aaggtacaac
     1201 cgtctcacat gttcagcgat tgcatcattc gttaatcgca gctggtttgg aaagtaacct
     1261 cactgtctat accggtggtc acgattatgc ttggtggcgc ggagcaatta ttgatgaatt
     1321 agcaaattat aattgcagga aggtatcaaa taataactct gtgtaaaata tcgttttatc
     1381 gtccgataac tctttctgca atattattat gggagactgt ctcatttatt tagtgatgtc
     1441 cttcatttat gtgatgaagg acattatatt actcataaca tttctcgttg aaaatttcag
     1501 caatacgcta caattgtttc ccgttgtttt ttaattttct catcaaaacc tctgcgatct
     1561 ctgcaggagt gggttgttca gccatttcag cggctacctg atctgaacag aagcgtagtg
     1621 aatcatcata aagcaggcga tttaccaaat cactggtcag tccatgcttg tccggaataa
     1681 tcccacaccc acgcttcgca acaatttctg cattaacaga gcgatctgca ccttctccaa
     1741 acacaatctg tggtattcca ctatacagcg ctgtcagggt attacctgcg ccaccgtgat
     1801 gaataaatcc atcagctcca ttaaggaata cacccatagg tatccagtca accagacgta
     1861 catttgatgg tagttttcgt aatccagtac gagcatttat tgcaagttgc aaaatgatat
     1921 cagcatcaac ttcatttgct gaatccataa cccatgaaat cagctccaga ccatcaacca
     1981 ttggttttac agtgccaagg ctgatgagta aacgtttccg atcagaatcc ctgtcccacc
     2041 atgtttcctt tacagcacct ccgttataag gaatatatcg cattgagata atcggttctt
     2101 cagcattttt aagaatgctc atacttgggg gagcaacatc aatccatgct aaatcacata
     2161 gtggaccgct gactccatgc cgcctgtaag cattgcttaa agaacggttt aacatctgaa
     2221 tatgggcaga tgtatgcgcg aatccaacag ccagcatcac tgaaggaatt ctatatttag
     2281 cagcaaccaa tgggcctgcc ggaccaagcg ggggatagac tattaaatct ggcctccatt
     2341 ttcctgcaaa atcgatgagg ttatctgcca tttcatcgct aaaaaaagag aaatgaccaa
     2401 taatattact ttttttcctc aactcttcct gatggcgata tccagcctct gaatctaaac
     2461 ttggagctgc atcaaaaaca accagtccag cttctgctgc tttattcgcg aatttgccag
     2521 cgctactaat tactacatca tgtccgatta cacgaaaggc ctgagccaga gaaatcaatg
     2581 ggtataacaa accgtacagc ggagggccaa taaaaagaat acgttttcgc attaaaaaag
     2641 tccgtcttct gcttacctgt ttat
//
LOCUS       DAPHCV010000271         2643 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.276, whole genome shotgun sequence.
ACCESSION   DAPHCV010000271 DAPHCV010000000
VERSION     DAPHCV010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.276"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..136
                     /locus_tag="RH033_004783"
     CDS             <1..136
                     /locus_tag="RH033_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDV2993835.1"
                     /translation="STSTQTRVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR
                     "
     gene            133..483
                     /gene="tnpB"
                     /locus_tag="RH033_004784"
     CDS             133..483
                     /gene="tnpB"
                     /locus_tag="RH033_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDV2993836.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTFRLNALTML"
     gene            514..2106
                     /locus_tag="RH033_004785"
     CDS             514..2106
                     /locus_tag="RH033_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDV2993837.1"
                     /translation="MSQKYLIRIAELESQLRQKDQQLSLVEETEAFLRSALARAEEKI
                     EEDEREIEHLRAQIEKLHRMLFGTRSEKLRREVELAEALLKQREQDSDRYSGREDDPQ
                     VPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGGELDYLGEVSAEQLELVSSALK
                     VIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPLYRQSEIF
                     ARQGVELSRALLSNWVDACCQLMTPVNDALYRYVMNTRKVHTDDTPVKVLAPGQKKAK
                     TGRIWTYVRDDRNVGSSSPPAVWFAYSPNRQGKHPEQHLRPFRGILQADAFTGYDRLF
                     SAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEIRGLPESE
                     RLAARQQRSKALLTSLHEWMVEKNGTLSKKSRLGEAFSYVLNQWDALCYYSDDGLAEA
                     DNNTAERALRAVCLGKKNYVFFGSDHGGERGALLYGLIGTCRLNGIDPEAYLRHILSV
                     LPEWPSNCVDELLPWNVVLTNK"
     gene            complement(2202..2549)
                     /gene="tnpB"
                     /locus_tag="RH033_004786"
     CDS             complement(2202..2549)
                     /gene="tnpB"
                     /locus_tag="RH033_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDV2993838.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPISGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
BASE COUNT          653 a          692 c          746 g          552 t
ORIGIN      
        1 atccacttcc actcagacca gagtcagtgc cagttcctgc aaggtggaat tccgtcacgg
       61 taacatgacg ctggaaaatc catcgccaga gctgctcacg gtgttgatcc gtgaactgac
      121 cgggagggga agatgatctc actcccatca ggtacccgta tctggctcgt tgccggcgtt
      181 accgatatgc gtaaatcctt caacggactg ggagaacagg tacaacatgt gctgaatgat
      241 aatcccttct ccggtcacct gtttatcttc cgtggccgac ggggtgacac cgttaaaatt
      301 ctttgggctg atgctgatgg tctgtgcctg ttcaccaaac gcctggagga aggccagttt
      361 atctggcctg cggtacgtga cggcaaggta tccattaccc gctcgcaact ggcaatgctc
      421 ctcgataagc tggactggcg tcagccaaaa acattccgcc ttaacgcact gacaatgttg
      481 taaaaaacgc ctggccgcat tataaaaacg gccatgagtc agaaatacct cattcgcatt
      541 gcagaactgg aaagccagct ccgtcagaaa gaccagcaac tgagtctggt tgaagagacg
      601 gaggccttcc tgcgctctgc actggcacgt gccgaagaaa agatcgaaga agatgaacgg
      661 gaaatagaac atctgcgggc tcagatagaa aaactgcacc ggatgctgtt cggtacccgt
      721 tctgaaaaac tgcgtcgtga agttgaactg gctgaggctc tgctgaaaca acgtgaacag
      781 gacagcgatc gttacagtgg gcgggaagac gatcctcagg ttccccgcca gttgcgacag
      841 tcgcgccatc gtcgtccgtt accggcacac cttccccgtg aaatacaccg cctggagcca
      901 gaagaaagct gttgcccgga gtgtggcggt gagctggatt atctggggga agtcagcgct
      961 gaacagctgg aactggtgag cagtgccctg aaagtgatcc gcacagaacg ggtaaaaaaa
     1021 gcctgtacaa aatgtgactg tattgttgaa gcaccggcgc cgtcccgccc gatagagcgt
     1081 ggtatcgcgg gccccggatt acttgcccgc gtgttaacgg gaaaatactg cgaacatctg
     1141 ccactgtatc gtcagagtga aatctttgcc cgccagggtg tcgaactgag ccgggcctta
     1201 ctctccaact gggttgacgc gtgctgccag ttaatgacac cggtgaatga tgccctgtac
     1261 cgttatgtaa tgaatacccg caaggttcac actgatgaca caccggtaaa ggtactggca
     1321 ccgggtcaga aaaaggcgaa aacagggcgt atctggacgt atgtccggga tgatcgcaat
     1381 gtgggttcgt catctcctcc agcggtctgg ttcgcgtact cgccgaaccg gcaggggaaa
     1441 cacccggagc aacaccttcg ccccttccgg ggtatcctgc aggcggatgc gttcacaggt
     1501 tacgacaggt tgttcagtgc agaacgtgaa ggtggtgcac tgacagaagt tgcgtgctgg
     1561 gcccatgccc ggcgaaaaat ccacgatgta tacatcagca gcaaaagtgc gacggcagaa
     1621 gaagccctga agcgaatcag tgaactgtac gccatcgagg atgaaatacg gggattacca
     1681 gagtcagagc gtcttgcagc caggcagcag cgaagcaaag cgttactgac gtcgctgcat
     1741 gaatggatgg tggagaagaa tggcacgctg tcgaaaaaat ccagactggg cgaagcgttc
     1801 agctatgtac tgaatcagtg ggacgccctc tgttattaca gtgatgatgg tttggcagaa
     1861 gcggacaata acacagcgga aagagcgctt cgtgcagtct gtctcggaaa gaaaaattac
     1921 gtgttcttcg gtagcgatca cggcggcgag cgtggagcac tgctgtacgg gctgatcggc
     1981 acctgccgtc tgaacggtat cgatccggaa gcgtatctgc gccatattct gagcgtactg
     2041 ccggaatggc cctccaactg tgttgacgaa ctcctgccat ggaacgtagt actcaccaat
     2101 aaataagcgt caatacggtg ctccgttgac gcttacaaga gatgtcgttc ataagcccgt
     2161 attttaccgg gcttattctg tgacaaccag gataaagaga tttacagcat ggtcagggag
     2221 gtcagcagcc gcttaggctg tcgccagtcg ataccttcca gcagcatcgc cagctgcgcc
     2281 tgcgtaagga acactttgcc atcacgggct gacggccagg cgaagcgccc acgctccagc
     2341 cgcttggtca ggaggcacag cccgtcaccg gtggaccaca gcagtttaac ctgactgccg
     2401 ctgcggcccc ggaaaatgaa aacatggccg gatatgggat cgtctttcag cgccgtctgt
     2461 actttcgcag ccaggccgtt gaagccattt ctcatatcgg tgataccggc aaccagccaa
     2521 attttggtcc cggaaggtaa cgggatcatc gtttcagttc ctgtatcagc agagtcagga
     2581 gtttttcgct gacattgcca ttgaagcgga gcgtcccgtg ccggaacgtt acctcacagc
     2641 tga
//
LOCUS       DAPHCV010000272         2642 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.277, whole genome shotgun sequence.
ACCESSION   DAPHCV010000272 DAPHCV010000000
VERSION     DAPHCV010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.277"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(118..336)
                     /locus_tag="RH033_004787"
     CDS             complement(118..336)
                     /locus_tag="RH033_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993839.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLINRLRERLVRHRDQH"
     gene            complement(468..1991)
                     /locus_tag="RH033_004788"
     CDS             complement(468..1991)
                     /locus_tag="RH033_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDV2993840.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGTVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTRSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNYRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(2327..2575)
                     /locus_tag="RH033_004789"
     CDS             complement(2327..2575)
                     /locus_tag="RH033_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993841.1"
                     /translation="MNNSIPERFILQCALFKNLEREVLMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVIS"
BASE COUNT          806 a          587 c          545 g          704 t
ORIGIN      
        1 aactgtgata acaacagagt tgacaactat ttgcgcaata attcctttct tcaatgatcc
       61 aggcgttcac tgccagcaac ccattcagcc gcatactgac agtgagaaac tgaaagatta
      121 gtgctgatcc ctgtgcctta cgagtctttc ccttaatcga ttaatcagat tgatcgccag
      181 ccaaacaagg gcattaagca aatttatcgt cacaagcact tctcccagcc cggtactggg
      241 ttttgcaata aggatgacca ggagagtgat cagcataaat gcaatggaaa agatgatata
      301 gagagtgaaa taatccagat tattactatt gttcatgcta ctaatggcaa tcctgtcaaa
      361 actttcaaac aattatagca aacaaaataa tgaacaatag taacaagagg caacgagcta
      421 attagctgtc tataattaca ccgcaccatg gccttactga tcaattttca ctcaaccaca
      481 accttcacct tcggtttaga aaggataatt ttgaccaggt cgatgtatgt taccggttta
      541 tagaaataat aaccctgctg aaatgtgata ttattttgat tcagatagtc aagttgttct
      601 ttcgtttcga caccttcagc gacgatactg atcgaaagtt tacgcgccag atccaataca
      661 caatccagaa tccgagttga ttctgggtct gcgttgacgc ggccaacgaa actgtgatca
      721 attttgatat aatcaatatg caggtcatgc agataagaaa gccctgagta gccagtaccg
      781 aaatcatcca gcgcgatgac aaaaccattt tcatgcagaa tgttcaaccg ctgaaccaga
      841 ctttcatcaa catttaatgg ctcacgctcg gtaacttcca gaacgaggtt taaatcgcgg
      901 cgggtaaaac tgtcacggta atttaagcac tcgtcgacaa acgtcggcga aataatatgc
      961 gaggcgctaa aattaattcc aatatgaaaa ccttccggca atttactcgc gatagcgttc
     1021 atctgtctgg caacctgatt catcaggctt cgcgtaagcg gaacgattaa tccggatttt
     1081 tcagcaagtg gaataaatgc cgcaggtgat atatatccac cgtgaggttg tttccagcgg
     1141 gctaacacct caactccccg caatgtccct tcccgaccat ttaccacagg ttggtaaaaa
     1201 gggacgatct caccattatt tatagcccgt cgcaggatct cttcaggcgt tgcgctttta
     1261 ttgaaatacc tatcaagcag gaaggcagcg gcgcaggcga ttaacagaat aaatatcaaa
     1321 atcccaaagc ctttatcgat aagtctgtag aagctaaaga gtggcggcgg gttaaagata
     1381 atagtgaaat gatattttgt ggagtgaacc ttctcggtat agggatagtg tccattaagc
     1441 gtcatcacat cacccgtagg tccaataatg tcatctgcca cgcgtaatac ataccttacc
     1501 cctttcaagg gtacattaag tgccccgcga atatgctgat cacttatcgt aaccaaaatg
     1561 cgcgtatctg caaattggct ctgatagagc aggataggta atctattaac ggtgtcgatt
     1621 gccggagcca acagtaaatt actgtccggg acaacaggaa tacgagacag caggacccga
     1681 ttccccggta aggatgtaca ccagaccgta ccctgtttga gaatgatgat tgtgcgcagg
     1741 tgaggtttca gagctgcttc agtgccaagc tgatattgct cctcgaggtc gcattccttc
     1801 ccggcaatgt taaccgctgt ttgagtcgcc tgtgacgctt cttcaagtat atgattgata
     1861 ttgttggcgg catatctcgc tcccgccagg tactctgctt ttgcggaata ccagagctgg
     1921 atgttcaaaa tagcgacacc ggttataaac aagcatatcg caacgaggat gggtatgagt
     1981 gtattgcgca ttcgcttgtc ccggacaaag ggtttaatac atgaaaatga cgttcattcg
     2041 ctcgcgctgg cgtctacaga gagcatggta gagagcatca caaatatcca cgccgaagat
     2101 aaaattttct ccaacttcat ctaacctatt aattattgag cacaatgtag gcagaattaa
     2161 acaaattagg tgggatatca ggcgtcaaga atgaaaaacg agcgctctcc attcctgttt
     2221 gccccctgat attgcggata tattaagaaa gcataacgcc tgaaatgctc actttgcacc
     2281 agcatggtga tacagctgat gtttattcta taaccttact caagttctaa gagatcacgg
     2341 attccctgtc attaataatg atatccgtta gtgtagtctt atgcatttca atgtactgca
     2401 atatttgcag atagagatca gaaagaatca cactagtttc atcttttaaa cgcaagcgta
     2461 aagcctgatc aataatatga ctatcaacat acccgtgcgt catgagcacc tccctctcga
     2521 gatttttaaa caaagcacat tgcaaaataa atctctcggg gattgaatta ttcatgtcag
     2581 cctcgtagga atattgaccc aataaaatta tgctgtgtat agtctaaagc gacagacaaa
     2641 aa
//
LOCUS       DAPHCV010000273         2626 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.278, whole genome shotgun sequence.
ACCESSION   DAPHCV010000273 DAPHCV010000000
VERSION     DAPHCV010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.278"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..850
                     /locus_tag="RH033_004790"
     CDS             <1..850
                     /locus_tag="RH033_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDV2993842.1"
                     /translation="QTLEKMSQTLFKSWFVDFDPVIDNALDAGNPIPEALQSRAELRQ
                     KVRNCADFKPLPAEIRSLFPSEFEETELGWVPKGWSFTALKNFGKIICGKTPTKSNKN
                     YYGEDFLFIKIPDMHGKVFVTNSHDKLSKLGSESQSNKIIPHGSICVSCIATVGLVSI
                     NAQDCHTNQQINSIVPNSPHYRNYLYFSMLEKYKIFHDLASGGSATLNMNTSVFSNIA
                     TLMPNNLVLKQFHKITEPWFEAILLNEYKLTSLASLRDTLLPKLISGELSLEDLPDLN
                     TDTEAA"
     gene            945..>2626
                     /locus_tag="RH033_004791"
     CDS             945..>2626
                     /locus_tag="RH033_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDV2993843.1"
                     /translation="MSLSFSEAKLEQAIIELLQDQGYQHLIGDNVPRSSLDQVIIEDD
                     LRHYLAARYQPDGITEEEIQRLIKQFTTLPASDLYESNKIFCRWLANGFLFKRDDRQQ
                     KDLYIELLDTRHLPAALRQIFDAEDVPLQQAAELPPSYINPPLNRIKIVNQLQISGKD
                     NQTRIPDGILYINGLPLVVFEFKSAVREQEASIGDAWKQLCKRYRRDIPQLFIYNALC
                     IISDGVNNRMGNLFAPYEYFYSWRKVTGNENREQDGIPSLHSMIQGLFHPVRLLDVIK
                     NFICFPDKAKHEVKICCRYPQYYAARKLYYSIKQARKPFGSGKGGTYFGATGCGKSYT
                     MQFLTRLLMKSVEFASPTIVLITDRTDLDDQLSAQMCNAKNYIGDDTIVPVTSREDLR
                     NQLAGRNSGGVFLTTIHKFTEDTELLSERSNIICISDEAHRSQVNLDQKVIVDKESGK
                     VRKTYGFAKYLHDSLPNATYVGFTGTPIDATLDVFGEVIDSYTMTESVQDEITVRIVY
                     EGRAAKVILDSSKLEEVEKYYEECANAGTNEWQIDESKKATATMNAVLGDEDR"
BASE COUNT          780 a          621 c          564 g          661 t
ORIGIN      
        1 ccaaacactc gaaaaaatgt cccaaaccct gttcaaatca tggtttgtgg attttgatcc
       61 ggtgattgat aacgcgctgg atgcaggaaa ccccatcccg gaagccctgc aatctcgcgc
      121 cgaattgcgt caaaaagtac gtaattgtgc agatttcaaa ccgcttccgg cggaaattcg
      181 ctcgcttttc ccaagtgaat ttgaagaaac ggagttgggt tgggtgccaa agggctggtc
      241 tttcactgca ttaaaaaact ttggtaaaat catatgcggt aaaacaccaa ctaaaagtaa
      301 caaaaattat tatggcgaag attttctttt tataaaaata ccagatatgc atggtaaagt
      361 ttttgtaaca aactcacatg ataaattaag taaactcggt agcgagtcac aatcaaataa
      421 aataattcct catggtagca tctgtgtaag ttgtatagcg actgttggtt tagtctctat
      481 taatgcgcaa gattgccata caaaccagca gatcaatagt attgtaccaa acagtccaca
      541 ttatcgaaat tacttatatt tttctatgtt agagaaatat aaaatatttc atgatttggc
      601 tagtggaggt agtgcaaccc tcaatatgaa tacaagtgtg ttctctaaca tagccacatt
      661 aatgccaaat aatctcgtgc ttaaacaatt tcacaaaata acagaacctt ggtttgaagc
      721 catattacta aatgaatata agttgacctc acttgcatct ctccgagata ccctgctccc
      781 gaaactcatc tccggtgagc tatccctgga agatcttccg gatctcaaca ccgatacaga
      841 agctgcataa cgcattttgc ccctgtaaaa tcaggggctt tctggtaagg ttttctactg
      901 atacgggaat gcttacccga aattagccag ggttggagcg caatatgagt ctctctttca
      961 gtgaagcaaa attagaacaa gcgatcattg aactgttaca ggatcaggga tatcaacacc
     1021 tgatcggcga taatgtccca cgttcaagtc tcgatcaggt catcatcgaa gacgatctcc
     1081 gtcattattt agcggcacgt taccagcctg atggcattac tgaagaagag attcagcgac
     1141 tgatcaaaca gttcaccacg cttccggcct ccgatcttta tgaaagcaac aaaatatttt
     1201 gtcgctggct ggcaaatggt tttctgttca aacgcgacga tcggcaacaa aaagatctct
     1261 atattgaatt gctcgacacc cggcatctac ctgccgcgct gcgccagata tttgacgccg
     1321 aagatgtccc gttgcaacag gcggcggaac tcccgccctc ctatattaat ccgccgctta
     1381 accggattaa gattgttaac cagcttcaaa tctccggcaa agataatcag actcgtattc
     1441 ctgacggcat tctctatatc aacggtctgc cactggtcgt ctttgaattt aaaagtgcgg
     1501 tgcgcgagca ggaagccagt attggcgatg cctggaagca gctctgtaaa cgctaccgcc
     1561 gggatattcc acaactgttt atctacaacg cgctctgcat tattagcgat ggcgtaaata
     1621 accggatggg caacctgttt gcgccctatg aatactttta ctcatggcgc aaagtcaccg
     1681 gtaatgaaaa ccgtgaacag gacggaattc catcattgca ctcaatgatt cagggactgt
     1741 ttcatccggt acgtctgttg gatgtgatta aaaactttat ctgcttcccg gataaagcca
     1801 agcacgaagt aaaaatttgc tgccgatatc cgcagtacta tgccgcccgc aaactctatt
     1861 acagcatcaa gcaagcgcgt aaacctttcg gtagcggtaa aggcggtacc tactttggcg
     1921 caacgggctg cggtaaaagc tacaccatgc aatttttaac ccgcttgtta atgaaaagcg
     1981 tggagtttgc cagcccgacc attgttttga tcaccgaccg taccgatctg gacgatcagc
     2041 tttctgcgca aatgtgcaac gccaaaaatt acattggcga tgacaccatc gttcccgtta
     2101 ccagccgtga agatttgcgt aatcaactgg cgggacgcaa tagtggcggt gtattcctga
     2161 caacgatcca taaattcacc gaagacaccg aactcctttc tgaacgcagc aatatcattt
     2221 gcatctcgga cgaagcacat cgcagccagg ttaacctcga ccagaaagtc atcgtcgata
     2281 aagaaagcgg aaaagtacgc aaaacgtatg gctttgcaaa atacctgcac gattcactgc
     2341 caaatgccac ctatgttggc tttaccggca caccgattga cgcgacgctc gatgtcttcg
     2401 gtgaggtgat cgacagttac accatgaccg aatccgttca ggatgaaatc actgtacgca
     2461 tcgtgtacga aggccgtgcg gctaaagtga tcctggactc cagcaaactg gaagaagtcg
     2521 aaaagtatta cgaagagtgc gcaaacgcag gcaccaatga gtggcaaatc gacgaaagta
     2581 aaaaagccac cgcaaccatg aatgcggtac tgggtgatga agatcg
//
LOCUS       DAPHCV010000274         2623 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.279, whole genome shotgun sequence.
ACCESSION   DAPHCV010000274 DAPHCV010000000
VERSION     DAPHCV010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.279"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            209..892
                     /locus_tag="RH033_004792"
     CDS             209..892
                     /locus_tag="RH033_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HDV2993844.1"
                     /translation="MNLKKTLLSVLIIVPLCLLAGCDYIEKASKVDDLVTQQELQKSK
                     IEALEKQQELDKRKIEHFEKQQTTIINSTKTLAGVVKAVKDKQDEFVFTEFNPAQTQY
                     FILNNGSVGLAGRVLAIDAVENGSVIRISLVNLLSVPVSNIGFHATWGNERPADAKAL
                     AKWQQLLFNTTLNSNLQLMPGQWQDINLTLKGVSPNNLKYLKLSINMANLQFDTVQPA
                     ETRQRKNKK"
     gene            936..>2623
                     /locus_tag="RH033_004793"
     CDS             936..>2623
                     /locus_tag="RH033_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDV2993845.1"
                     /translation="MNKIFKVIWNPATGSYSVASETAKSRGKKSGRSKLLISVLVASG
                     LLSSFGALANNYDGQPTDYGDGSAGDGWVAIGKGAKANTFMNTSGASTALGYDAIAEG
                     EYSSAIGSKTLATGGASMAFGVSARAMGDRSVALGASSVANGDRSMAFGRYAKTNGYT
                     SLAIGDSSLADGEKTIALGNTAKAYEIMSIALGDHANASKEYSMALGASSAASAANAI
                     AVGRNSAAAGVDSLAFGRLSAANAANAIAMGAESKAAENATAVGTNAEANGLNSIALG
                     SGSIADVDNTIALGNQSQAVAAGAIAIGQGNKADGANAIALGNGSITGGVNAIALGQG
                     SYAGLENGTAIGAQASAQGKNSVALGAGSVATDADTVSVGNTTAQRQIVNMAAGDIST
                     TSTDAINGSQLYAISKSVADNLGGGATVNAQGVVTSPNYRLKSGIFGTVGDALTGLDN
                     NTLQWDSLKKAYSAAHGTDTTSTITNVKDGAISDTSKDAVNGSQLKTTNDNVATNTTN
                     ITTNTNSINTLTDSVGDLKDDALLWNGTAFSAAHGTEATSKITNVKDGDLTAGST"
BASE COUNT          776 a          573 c          650 g          624 t
ORIGIN      
        1 tgacaccgat cattaaaatc cgcgcgatta atattattgg ctacaaactg aaatcgtttg
       61 acatttaata acatattcgc acaaccaatt aatacaaaat ttctctaaca aatttatttt
      121 gcggatatgc cccactccaa tcattcataa aaaaatgatt gtgccgtaat tatcaactgt
      181 atcgggatta tgaaaaggaa ttcatcctat gaacctaaag aaaacactgt taagcgtgtt
      241 aataatagtg cctctctgct tattggcagg gtgtgactat attgaaaaag cgagtaaggt
      301 cgacgacctc gttacacagc aagagttgca aaaaagcaaa attgaggcgc ttgagaaaca
      361 acaggaactc gataagcgaa agatagagca ttttgaaaaa caacaaacta ctatcataaa
      421 tagtaccaag acgctcgctg gcgtggtgaa agcggttaaa gacaagcagg acgaatttgt
      481 ctttacagaa tttaacccag cacaaacaca atatttcatt ttaaataacg gatcggtcgg
      541 tttggcaggc cgggtgctgg ctattgatgc ggttgaaaat ggcagtgtta ttcgtatttc
      601 gctggttaac cttttaagcg ttcccgtatc aaatattggc tttcatgcga catggggaaa
      661 tgaaagaccc gccgatgcca aagctctggc aaagtggcaa caattacttt tcaacaccac
      721 cctgaattcc aatctgcaat taatgcctgg tcaatggcaa gacattaatc tgacattaaa
      781 aggcgtttcg cctaataacc tgaagtatct gaagttgtcc atcaatatgg ccaatctcca
      841 gttcgacact gttcaacctg ctgaaactcg gcagcgaaaa aacaaaaaat aaatgcagct
      901 tctgcgctta tatcaaggaa tagagagcat caataatgaa caaaattttt aaagttatct
      961 ggaatccggc gacgggcagt tacagcgttg ccagcgaaac ggcgaagagc cgtggcaaaa
     1021 aaagcgggcg cagtaagctg ttaatttctg tactagttgc gagtgggttg ttgtcgtcgt
     1081 ttggagcgtt agctaataac tacgacgggc agccaactga ttatggcgat ggctcagcag
     1141 gtgacggctg ggttgctatc ggtaaagggg caaaagcaaa tacctttatg aacactagtg
     1201 gcgcgagtac agctttagga tatgacgcga tagccgaagg tgagtacagt tctgccatcg
     1261 ggtcaaaaac ccttgcaact ggtggagcat ccatggcgtt cggggttagt gcaagagcaa
     1321 tgggtgacag aagtgtcgcg ctgggtgcat cgtcagtagc aaatggcgat cgttcgatgg
     1381 cttttggtcg ttacgcaaag acgaatggtt atacatctct tgctattggg gactcctccc
     1441 ttgccgatgg tgaaaaaact attgcgttag gaaatacggc taaagcttac gaaattatga
     1501 gcatcgccct cggtgatcat gccaatgcgt caaaagaata ttcaatggcg ctgggtgcaa
     1561 gtagtgccgc atctgcggct aacgcgattg cagtaggccg caacagtgct gcagctggcg
     1621 tagacagcct cgcattcggt agactatccg ccgccaacgc cgcaaacgcg attgctatgg
     1681 gtgcagagag caaagctgct gaaaatgcga cggcagttgg tactaacgcg gaggcaaatg
     1741 gacttaatag tatcgcgttg ggctccggca gtattgcaga tgtcgacaac actattgctc
     1801 tgggcaatca aagtcaggca gtagcggctg gcgcaattgc catcggtcaa gggaataaag
     1861 ctgacggcgc aaatgctatc gcgctgggta atggtagtat tacaggtggt gtaaatgcta
     1921 ttgctcttgg acaaggcagt tatgccggtt tagaaaatgg cactgcaatt ggtgctcaag
     1981 ccagtgctca ggggaaaaat tcagttgctc tgggtgctgg ttctgtagcg actgacgcgg
     2041 atactgtttc tgtgggtaac acaacagctc agcgacaaat tgtcaatatg gcagcaggtg
     2101 atatcagcac taccagtact gatgccatca atggatcaca gctttatgct atcagtaagt
     2161 cagtagcgga caatcttggt gggggggcta ccgtcaatgc gcaaggcgtc gttacttccc
     2221 caaattacag gctgaaaagt ggtattttcg gcactgttgg cgacgcctta acgggcctgg
     2281 acaataatac gttacaatgg gactccctta aaaaggcata tagtgcggca catggtacag
     2341 atactaccag taccatcacc aacgttaaag acggcgctat ttctgatacc agtaaggatg
     2401 cggttaacgg ttctcagctg aaaaccacca acgataacgt agcgaccaat actaccaata
     2461 tcaccaccaa cactaacagt atcaataccc tgacggattc cgttggcgac cttaaagacg
     2521 atgccctgct gtggaatggc accgcgttca gcgccgcgca cggcaccgaa gccaccagca
     2581 aaataaccaa cgtcaaagac ggtgacctga cggctggcag cac
//
LOCUS       DAPHCV010000275         2618 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.280, whole genome shotgun sequence.
ACCESSION   DAPHCV010000275 DAPHCV010000000
VERSION     DAPHCV010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.280"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(25..1875)
                     /gene="traN"
                     /locus_tag="RH033_004794"
     CDS             complement(25..1875)
                     /gene="traN"
                     /locus_tag="RH033_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HDV2993846.1"
                     /translation="MKRILPLILALVAGMAQADSNSDYRAGSDFARQIQGQGTGSIQG
                     FKPQESIPSYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEINRSEFTNYTCERDTMVEEYCTRT
                     ASITGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTAPVTGTVESASLSVYAAFFFL
                     NSRYTFMNTTFNVGLANGQSDTYPLSGATGLQVTQGQVLTGSGCTANGNCLPHGNGDR
                     KVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVESCPFNKADGVLTGTECSEPGGTKT
                     GVMEGKPWNITQACWAYRDKYVTQSADNGTCQKYVDNPACTLASRQCAFYSDEGTCLH
                     EYATYSCESRTSGKVMVCGGDVFCLDGECDKAQSGKSSDFGEAVSQLAALAAAGKDVA
                     ALNGVDVRAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLAKCNSEEKALGKAKDNKLT
                     VSVGEFCSKKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNGQLRIGFGSAKHPDCRGIT
                     VDELQKIQFDRLDFTNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            complement(1872..2510)
                     /gene="trbC"
                     /locus_tag="RH033_004795"
     CDS             complement(1872..2510)
                     /gene="trbC"
                     /locus_tag="RH033_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HDV2993847.1"
                     /translation="MNRKIFALFALTAGMHLAVHASENVNTTENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVAHDLLAGKGDSGK"
BASE COUNT          567 a          789 c          591 g          671 t
ORIGIN      
        1 tttcccccgg ttattttcca taccttactg tcctgcctgt ttcagctggt cagcaatctg
       61 ctctttcact ttctgcgtca gaacgccact gtccgggatt ttctggttat tcatcaggtc
      121 ttcgtagaag ttagtgaaat ccagtctgtc gaactgaatt ttctgcagct catcaaccgt
      181 aatcccccga cagtcaggat gcttcgcact gccaaacccg atacgcaact gcccgttacg
      241 cccctgctgc tggactatct gcgccagctt cgaatcaaac tggcagtagc tgcgcttttt
      301 ctgcagacag acacccagca ctttcttcga acagaactct ccgacactga cggtcagttt
      361 gttgtccttt gcctttccca gcgccttttc ttcgctgttg cacttcgcca gacccacatc
      421 ctgaccccag ccaccatcct tacagcagtt actgaagccg gctgcggcct tacggcagaa
      481 tttcgcctca cccgtgaaag ccctgacatc cacaccattc agggcggcaa catccttacc
      541 tgcggcggca agcgcagcca gctgtgatac tgcttcacca aaatcactgc tttttccgct
      601 ctgtgcctta tcgcattcac cgtcaaggca gaagacatca ccaccacaga ccatcacttt
      661 tcctgatgtt ctggactcgc aggagtacgt ggcgtactca tgcagacagg ttccctcatc
      721 ggaataaaac gcgcactgac gtgaggccag cgtacaggcc ggattatcga catatttctg
      781 acaggtgccg ttatctgcgg actgagtgac atatttatcc cggtatgccc agcatgcctg
      841 tgtgatgttc cagggtttac cttccatcac tccggttttc gtaccacccg gctctgaaca
      901 ctcagtgcct gtcagcacgc catccgcttt attaaacgga caactctcca cccactccac
      961 ccggggaacc cattctttat cacggacctt catcctgagt ttcagcgtaa aggtggaagc
     1021 accactgaca agcgattcat agactttcct gtcaccattc ccgtgcggga ggcaattacc
     1081 gttagcagta cagccactac cggtcagaac ctgcccctgt gtcacctgca aacctgtcgc
     1141 cccggacagg gggtaagtat ccgactgccc gtttgccagg ccgacattaa aggttgtatt
     1201 catgaaggta taacgggagt tcaggaagaa aaaagcggcg tatacagaca atgatgcact
     1261 ttccactgta ccggttaccg gagccgtgac actgaacacc agtttcagcc cgttcatact
     1321 gaaccggaac tgtgagtgcg gtatcgtcac ttcacgatat tcatccgtgt atttccagtc
     1381 cccggtgata ctggcagtcc gtgtgcagta ctcctccacc atcgtgtccc gctcacaggt
     1441 atagtttgtg aattcactgc ggtttatctc ctgcgcactg cactgctgtc cggtatttcc
     1501 cacaatgctg tcagcccggt tcaccacatc cctgccggtc tgaatgaacg gtgcatccgg
     1561 tgacagaatg tctttcggct tgttcatgaa ggactctgtg atggtttttc cggtttcacc
     1621 ggtcgcccat tccgtggtgc cgtcattttt caggccgcca tccccgccgg cagtcacgcc
     1681 accgtaatat tttgtctcgt ccggattcgc gttatagctg gggatactct cctgtggctt
     1741 aaagccctga atactgcctg taccctgccc ctgaatctga cgggcaaaat cagaaccggc
     1801 ccggtaatca ctgttgctgt cagcctgtgc catgccggca accagagcca gtatcagagg
     1861 taaaatacgt ttcatttccc ggaatctcct ttccctgcca gtaaatcatg agccacctgc
     1921 cggcagtcac ccgtggcggc cactttctcc agcgcctggc cgacccgcag gtttccccgg
     1981 atgatgtcgt atccctgact gcagaacacc accagtgccg gtacactgcg aatgccgtac
     2041 tgtgaaaaca gcgtcgggtc gatctgaaca ccatcagtcg cgccgtcttt caccagggac
     2101 agcacggctt cagcggtggt cttcaggtca ttgttcacca tgccccgcag tgtggcgggt
     2161 ataccgtagt gccgggtttc gcccagcatc cgtttcagcc cctcttcggg aatggaaaac
     2221 gacacaaaat acagggcacc ctgccggggt ttcccgtcct gcgaagcctg ctgtttacga
     2281 accagctcat ccagaaaatg gttatctgaa cgctgaaggg ggttttccag cacctgtttc
     2341 tccgcccagg ctttcagctg atggtcaggt ttctctcgca gttgtctgct taaattttcc
     2401 tgctgcttca ggaactggcg gttttcagtt gtgttcacat tttctgaagc atgaacagca
     2461 agatgcatcc ctgctgtcag agcaaaaagt gcaaatattt tccggttcat gatatttctc
     2521 actttctgct atttacaggc atgaactcat cagaaaaaag ataattgaga aaaacagcca
     2581 caaacgcaca tgtaaatgtc accttcagat ataatctg
//
LOCUS       DAPHCV010000276         2617 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.281, whole genome shotgun sequence.
ACCESSION   DAPHCV010000276 DAPHCV010000000
VERSION     DAPHCV010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.281"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(289..402)
                     /locus_tag="RH033_004796"
     CDS             complement(289..402)
                     /locus_tag="RH033_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDV2993848.1"
                     /translation="MHEPPVQSDRCALSGNYRLESNPERHAKAPLAAATGN"
     gene            1051..1200
                     /locus_tag="RH033_004797"
     CDS             1051..1200
                     /locus_tag="RH033_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993849.1"
                     /translation="MPSEYDEQRGSTDTQLPGEYDLFDLMAKVTEEILHAPVDSGPPV
                     GREAL"
     gene            2103..>2617
                     /locus_tag="RH033_004798"
     CDS             2103..>2617
                     /locus_tag="RH033_004798"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993850.1"
                     /translation="MRERYLNKISNFDTARIVLFIAIQIISCIFMFWGAIPALIVFFS
                     YLAMRKECNLKPIEKSKDYISVYMLLTAALVLFLFVYKAGMFRRGGDYFLLVFVFILL
                     YVYHLAFHKLWLRTIDENSSIIIENGIFNFNRKNASVGFSRKDVNNIDTLLKLNEARK
                     EGIISEDEFNK"
BASE COUNT          667 a          583 c          581 g          786 t
ORIGIN      
        1 aaaaaaaaga ctaaatattc ttgacgactg atttttttgc taactaaagg taaagcaagc
       61 ttatgaatct ttgataagat tgaatatctt cttgtcattg attttttatc gtcgtaatct
      121 tcttccctgc aaacgaaaaa accacccggc aaggtggttt ttcgaaggtt cgcaagagtt
      181 gacgctcttt tgaaccgcgg taactggctt ggaggagcac agtcaccaaa acttgtcctt
      241 tcagtttagc cttaaccggc gcatgacttc aagactaact cctctaaatc agttaccagt
      301 ggctgctgcc agtggtgctt ttgcatgtct ttccgggttg gactcaagac gatagttacc
      361 ggataaggcg cagcggtcgg actgaacggg gggttcgtgc atacagtcca gcttggagcg
      421 aactgccttc ccggaactga gtgtcaggcg tggaatgaga taaacgcggc cataacagcg
      481 gaatgacacc ggtaaaccga aaggcaggaa caggagagcg cacgagggag ccgccagggg
      541 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca ccactgattt gagcgtcaga
      601 tttcgtgatg cttgtcaggg gggcggagcc tatggaaaaa cggctttgcc gcggccctgt
      661 cgcttccctg ttaagtctct tcctggcatc ttccaggaaa tctccgcccc gttcgtaagc
      721 catttccgct cgccgcagtc gaacgaccga gcgtagcgag tcagtgagcg aggaagcgga
      781 atatatcctg tatcacatat tctgctgacg caccggtgca gccttttttc tcctgccaca
      841 tgaagcactt cactgacatc ctcatcagtg ccagcatagt aagccagcat agtaagccag
      901 catagtaagc cagcatagta agccagcata gtaagccagc atagtaagcc agcatagtaa
      961 gccagtatac actccgctag cgctacgtga ctggttcagg gctgcgcccc gaaacccgct
     1021 aacaacctct ctgctactgg atatcacccc atgccatctg aatatgatga acaacggggt
     1081 tcgactgata cgcagcttcc cggcgaatat gacctttttg atttaatggc gaaggttact
     1141 gaagagatcc tgcatgcccc tgtggattct gggccgcctg tcggccggga agcgttatag
     1201 ctttttcgct tgcccgcgta cgtggtaata cgccggtatt accttttatt tctttatttt
     1261 catgtaatta cagtgggttt ttaatacctg ggtattactc gtgaaaacga aacattcctt
     1321 ttttttcttt taattcatgc agttgcgatg aaaatcgcgg ctgcgttagg tgtatgacca
     1381 ttttgaggtt gaaaaataat caaccatgga gggtgattta cgcaggttaa ggggagatcg
     1441 tgacaaaaaa agcgttcctc actgtatcgt tcatgcctgc atgaacgggt tattcttcct
     1501 gactacagaa ttacatcgct ccttcagttc atacatcact tttacaacac ttttacaaca
     1561 ctccttcggc ctgtacatca cctttacaac acttttgcat cacttttaca tcatttatac
     1621 atcactttta catcattcct tcagctcata cgtcactttt acgtcagttc catcctctgt
     1681 gccatttgta catccgcccc ccttcctgaa cgtgctgaaa acggtttttc cggctgtgcc
     1741 ggcaacgcct cacggccacg caataaattg cgtccccgtt caccgttttc gggtaagaag
     1801 aacatcggaa acaggactta ccccggctga atggcgtgaa tttccgcact atgcacttgc
     1861 gcgcatactc atgcatgccg taaaaacaga gactgcgcgt ttctggcggg ttttcgggtg
     1921 gtttgttgcc tgttttaccg gtttcccgtc agaaacgccc tcagagcctc tcaggcggtg
     1981 cacgcaaggg gtgttatggt aaatgcccca tattcagtta ctgcttttct attttttgaa
     2041 ataatattaa tatcaggcga ttgattttaa attaattaac tatgattgaa tggtggtgat
     2101 ttatgcgcga acggtattta aataaaatat ctaattttga tacggcaaga atagtgttgt
     2161 ttatagccat tcaaattatc tcttgtattt ttatgttctg gggggctatt cctgccttga
     2221 ttgttttttt ctcatattta gccatgagga aggaatgtaa tttaaagccg atagagaagt
     2281 ctaaggatta catatccgtt tacatgcttc ttacagccgc tttggtactg tttcttttcg
     2341 tgtataaagc tggcatgttt agaaggggcg gtgattattt ccttctcgtt tttgttttta
     2401 ttttgcttta tgtttaccat ctcgcttttc ataaattatg gttgcgaacg atagatgaaa
     2461 actcatcaat aataattgaa aacgggattt ttaattttaa tcgtaaaaat gcatctgtag
     2521 gatttagtcg gaaagatgta aataacattg atactcttct taaattaaat gaggctagaa
     2581 aagaaggtat tataagtgaa gatgagttta ataaatt
//
LOCUS       DAPHCV010000277         2598 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.282, whole genome shotgun sequence.
ACCESSION   DAPHCV010000277 DAPHCV010000000
VERSION     DAPHCV010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.282"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            351..494
                     /locus_tag="RH033_004799"
     CDS             351..494
                     /locus_tag="RH033_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HDV2993851.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            507..716
                     /gene="cspE"
                     /locus_tag="RH033_004800"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             507..716
                     /gene="cspE"
                     /locus_tag="RH033_004800"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HDV2993852.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            882..1691
                     /gene="rlmA"
                     /locus_tag="RH033_004801"
     CDS             882..1691
                     /gene="rlmA"
                     /locus_tag="RH033_004801"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HDV2993853.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1688..2254)
                     /gene="mntP"
                     /locus_tag="RH033_004802"
     CDS             complement(1688..2254)
                     /gene="mntP"
                     /locus_tag="RH033_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HDV2993854.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          701 a          603 c          621 g          673 t
ORIGIN      
        1 tcgatagccg ttaactgact gttttatgag aaaaagtgat ataacttttt attcattgca
       61 tagcaaaaaa tgtgatattg cacgcactat gtaataactt ctcccactgg cctggaacaa
      121 ctgaacttat tgaactatgt tagaaaatac gccagtttaa gtatcagcct gaactggcaa
      181 ggttaagcac aatgatatat cggcgcgtat tccgttgcat aagtgtgcaa aaaaagtgga
      241 agacgtatcg agatttgtgc gtctgatcga gacatgttta aaaatggctt gccataatta
      301 acgttgtatg tgataacaga tttcgggtta aacgaggtac agttctgttt atgtgtggca
      361 ttttcagtaa agaagtcctg agtaaacacg ttgacgttga ataccgcttc tctgccgagc
      421 cttatattgg tgcctcatgc agtaatgtgt cagttttatc tatgttatgc ctgcgggcga
      481 agaaaacaat ctaaggaatt tttcaaatgg caaagattaa aggtcaggtt aagtggttca
      541 acgagtctaa aggttttggc ttcattactc cggctgatgg cagcaaagac gtgttcgtac
      601 acttctctgc tatccagggt aatggtttca aaactctggc tgaaggtcag aacgttgagt
      661 tcgaaattca ggacggtcag aaaggtccgg cagctgttaa cgtaacagct atctgatcga
      721 atccactgat ctgaagtgtg aatacgcttc aatctcgcta taaagcctcg tcgaatgcga
      781 ggctttttac tttgctttat cttcgctcct ggcgttcgga tatttgcccg ccgcgtgatt
      841 cgcgttacac ttgcggcctt tagtatcctg ccggagttgt catgtcgttt tcctgtccac
      901 tttgccatca gcctctttcg cgtgaaaaaa acagctatat ctgtccccag cgacatcagt
      961 ttgatatggc gaaagaaggg tatgtcaatc tgctgcccgt tcagcataaa cggtctcgtg
     1021 atccgggcga cagcgcggaa atgatgcaag cacgccgcgc attcttagat gccggacatt
     1081 atcagccgct gcgtgatgca attgtcgccc aactgcggga acggcttgat gaaaaggcca
     1141 cagcggtgct ggatattggc tgtggtgaag ggtattacac acacgcattt gccgatgcgc
     1201 tgcccgaaat caccacgttt ggcctggatg tctcgaaggt agcgataaaa gcggcggcga
     1261 aacgctatcc gcaggtcact ttttgtgttg cttccagcca ccgtttgccg ttttccgata
     1321 ccagtatgga cgccataata cgtatttacg cgccgtgtaa agcagaagaa ttagcgcgag
     1381 tagtgaagcc tggcggctgg gtcattactg ccacgccggg accgcgacat ttgatggagc
     1441 tgaaggggct gatttacaat gaagtacatc ttcatgcacc acatgcagaa caactggaag
     1501 gttttacatt acagcagagt gatgagttgt gttatccgat gcgtcttcgc ggtgatgaag
     1561 ccgtcgcatt attgcagatg acgccgtttg cctggcgtgc gaagccagaa gtctggcaaa
     1621 cactggcagc aaaagaagtg ttcgactgcc agacggactt taatattcac ctctggcagc
     1681 gttcttatta accgtggaag tgcgtccaga ggatctggac gccgatgccg atcagcacca
     1741 gcccgccgag aatttccgct tttttcccaa taattgagcc gataaagcga ccaaccatca
     1801 tccctaatgt cgacataatc aaggttgcac aaccaatggc caatgcggtc gcgataatgt
     1861 tgacctgcag gaaggcgaga ccaacaccca cagccatggc atccaggctg gtggcaatcg
     1921 cggtggttac cagtagccag aaaccgtgcc gacggcgcgg ctcttcatct tcatcatctg
     1981 cgccacgaaa accctcaata atcattcgcc cgccgaggaa tatcagcagc acaaacgcaa
     2041 tccagtgatt ccattcaagg acaaaccggc tggctaacat gcccattccc cagccgatca
     2101 gcggtgtcag ggtttcgacg gcaccaaaaa taaggccggt tcgcaatgct tcagaaaatt
     2161 tcggtttatg gagggtggca cctttaccga ttgatgcagc aaatgcatcc atcgacatac
     2221 caaacgcaag aagaacagta gcagtgatat tcatgacaac gtcctgaccg gggtatccat
     2281 aaaacacatc atcgccccca gcaaacatgc atacatgaac aacaagccgc gatgggtttt
     2341 cgcgttcatg caggcatagc gattgatgtg catatggtct cgcctgactg aaagtgttca
     2401 gtccgtacgt gccacgtttt gcaacgagta tgttgacacg tacatttccg gaatctggaa
     2461 atcggctact ccccaaatga gagcgcaacc ttaacatatt ttgagaatat aaaacaactg
     2521 ttggaatata ttatttcatt gctttattga taacgatttt catttagatt tatatatcaa
     2581 tgaaacgtta aaataaat
//
LOCUS       DAPHCV010000278         2591 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.283, whole genome shotgun sequence.
ACCESSION   DAPHCV010000278 DAPHCV010000000
VERSION     DAPHCV010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.283"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..87
                     /locus_tag="RH033_004803"
     CDS             <1..87
                     /locus_tag="RH033_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HDV2993855.1"
                     /translation="AVMFSQPASQLLAGARMDVYVIRDGEGN"
     gene            87..671
                     /locus_tag="RH033_004804"
                     /pseudo
     CDS             87..671
                     /locus_tag="RH033_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
     gene            672..2429
                     /locus_tag="RH033_004805"
                     /pseudo
     CDS             672..2429
                     /locus_tag="RH033_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="cleaves origin of transfer and unwinds; oriT
                     relaxase; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            2449..>2591
                     /locus_tag="RH033_004806"
     CDS             2449..>2591
                     /locus_tag="RH033_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993856.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLD"
BASE COUNT          589 a          666 c          874 g          462 t
ORIGIN      
        1 gccgtgatgt tcagccagcc tgccagccag ttactggcag gtgcgcgcat ggatgtgtat
       61 gtcatccgtg acggggaggg caactgatgg ccagtatcaa taccattgtg aaacgcaagc
      121 agtacctgtg gctggggatt gtggttgtcg gtacagcctc cgcgattggt ggggcactgt
      181 atctgtctga tgtggacatg tccggtaacg gtgaaaccgt ggctgaacag gagcctgtgc
      241 cggatatgac cggtgtggtg gatacgacct ttgatgacaa ggtgcgtcag catgccacca
      301 ccgagatgca ggtgacggca gcgcagatgc agaagcagta tgaggaaatc cgtcgtgagc
      361 tggatgttct gaacaaacag cgcggtgatg accagcgtcg tattgaaaag ttgggacagg
      421 acaatgccgc cctggcagag caggtaaaag ccctgggtgc taatcccgtc acggcgaccg
      481 gtgagcctgt accgcagatg cctgcctcac cgcccggccc ggaaggcgaa ccacagccag
      541 gaaacacccc cgtatccttc ccgccgcagg gcagcgttgc tgttccaccg ccgacggcgt
      601 tttatcccgg gaatggtgtc acgccaccac cacaggtgac gtaccagtct gtgccggtgc
      661 ctaaccggat accggagcac tccgtgacgg agttcagtca cagccaggaa gcgaaactgg
      721 cagaagcgca gcagaaggcg atgctgaaag gtgaggcttt tccggatatt cccatgacac
      781 tgtatgaagc cattgtccgc gattataccg gcaggacgcc ggaagcacgg gagcagacgc
      841 tgattgtcac gcatctgaat gaggaccggc gcgtactgaa cagcatgatt catgatgcac
      901 gggaaaaggc cggtgagctg ggaaaagagc aggtcatggt gcctgtcctg aacacagcga
      961 atatacgcga cggggagttg cgtcgtctct ccacctggga gacacatcgg gacgcacttg
     1021 ccctggtgga taatgtgtat caccggattg ccggtatcag caaggatgac gggctgataa
     1081 ccttgcagga tgcggaaggt aacacgcggc tgatttcgcc ccgggaggcg gtggctgaag
     1141 gcgtcacact gtacactccg gacaccatcc gggtgggaac cggtgaccgg atgcgcttca
     1201 cgaagagtga ccgggagcgc ggttatgtgg ccaacagcgt ctggacggtg acagcggttt
     1261 ccggtgacag tgtcacgctg tcggacggac agcagacccg ggtgattcgc cccggtcagg
     1321 agcgggcaga gcaacatatt gacctggcct atgccatcac cgcccacggt gcgcaggggg
     1381 caagtgaaac ctttgccatt gcgcttgaag gcacggaagg taaccggaaa cagatggccg
     1441 gttttgagtc agcctacgtg gccctgtcgc gtatgaagca gcatgtgcag gtgtataccg
     1501 ataaccgtca gggctggacg gatgccatta acaatgccgt acagaaagga actgcccacg
     1561 atgtgctgga gccgaaatcg gaccgggagg tcatgaatgc agagcggctg ttcagtacgg
     1621 cgcgggagct gcgggacgtg gtggccggcc gtgccgttct ccgtcaggca gggctggccg
     1681 ggggagacag tcctgcacgg tttattgcac ccgggcgcaa atatccgcag ccgtatgtgg
     1741 cactgccggc ttttgaccgt aacggcaagt cagctggtat ctggctgaac ccgctgacca
     1801 cggatgacgg aaacgggctg cggggcttca gtggtgaagg ccgggtgaaa ggcagcgggg
     1861 atgcgcagtt cgtggccctg cagggcagcc gtaacggaga gagcctgctg gctgataata
     1921 tgcaggatgg tgtccagatt gcccgtgata atcctgacag cggtgtggtg gtaagaatcg
     1981 ccggtgaagg tcgtccgtgg aatcccggta ccatcaccgg tggtcgcgtg tggggggata
     2041 tcccggacaa cagcgttcag ccgggagccg gaaatggcga accggtcacg gcagaggtac
     2101 tggcacagcg gcaggctgaa gaggccatcc gccgtgaaac ggaacgccgt gcggatgaaa
     2161 ttgtccgtaa aatggcagag aacaaacctg acctgccgga cggcaaaaca gagcaggctg
     2221 tcagggatat tgccgggctg gagcgtgaca ggtctgccat atctgaacgg gaagctgcgc
     2281 tgccggagag tgtactgcgt gaaccacaac gggtgcggga agcggtccgg gaggttgccc
     2341 gggaaaatct gctgcaggag cgactgcagc agatggagcg ggatatggtt cgtgacctgc
     2401 agaaagagaa aaccctgggc ggagactgac acaggaagat aaacgctgat gacaacggat
     2461 aacacgaaca caacacgtaa tgattcactg gctgcccgga ccgatacctg gttgcagtcg
     2521 ttgctggtct ggtcacccgg acagcgggat atcatcaaaa cggtggcact ggtgctgatg
     2581 gttctggacc a
//
LOCUS       DAPHCV010000279         2589 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.284, whole genome shotgun sequence.
ACCESSION   DAPHCV010000279 DAPHCV010000000
VERSION     DAPHCV010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.284"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            51..401
                     /gene="tnpB"
                     /locus_tag="RH033_004807"
     CDS             51..401
                     /gene="tnpB"
                     /locus_tag="RH033_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDV2993857.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSSRNSLTML"
     gene            432..2066
                     /locus_tag="RH033_004808"
                     /pseudo
     CDS             432..2066
                     /locus_tag="RH033_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362703.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(2161..2349)
                     /locus_tag="RH033_004809"
     CDS             complement(2161..2349)
                     /locus_tag="RH033_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FaeA/PapI family transcriptional regulator"
                     /protein_id="HDV2993858.1"
                     /translation="MKDNILTFLTELKTPCRTTEIAIHFGLSAYQARYYLMTLEKEGK
                     IKRSPVRRGASTLWEMNS"
BASE COUNT          642 a          646 c          693 g          608 t
ORIGIN      
        1 tcgccagagc tgctcacggt gttgatccgt gaactgaccg ggaggggaag atgatctcac
       61 tcccatcagg tacccgtatc tggctcgttg ccggcgttac cgatatgcgt aaatccttca
      121 acggactggg agaacaggta caacatgtgc tgaatgataa tcccttctcc ggtcacctgt
      181 ttatcttccg tggccgacgg ggtgacaccg tcaaaattct ttgggctgat gctgatggtc
      241 tgtgcctgtt caccaaacgc ctggaggaag gccagtttat ctggcctgcg gtacgtgacg
      301 gcaaggtatc cattacccgc tcgcaactgg caatgctcct cgataagctg gactggcgtc
      361 agccaaaaac atccagccgt aactcactga caatgttgta aaaaactcct gaccgcatta
      421 taaaaacggt catgagtcag aaatacctca ttcgcatcgc agagctggaa aggttgctct
      481 ctgagcaggc tgaagccctc cgtcagaaag accagcaact gagtctggtt gaagagacgg
      541 aagccttcct gcgctctgca ctgacacgtg ccgaagaaaa gatcgaagaa gatgaacggg
      601 aaatagaaca tctgcgggct cagatagaaa aactgcgccg gatgctgttc ggtacccgtt
      661 ctgaaaaact gcgtcgtgaa gttgaactgg ctgaggctct gctgaaacaa cgtgaacagg
      721 acagcgatcg ttacagtggg cgggaagacg atcctcaggt tccccgccag ttgcgacagt
      781 cgcgccatcg tcgtccgtta ccggcacacc ttccccgtga aatacaccgc ctgtagccag
      841 aagaaagctg ttgcccggag tgtggcggtg agctggatta tctgggagaa gtcagcactg
      901 aacagctgga actggtgagc agtgccctga aagtgatccg cacagaacgg gtaaaaaaag
      961 cctgtacaaa atgtgactgt attgttgaag caccggcgcc gtcccgcccg atagagcgtg
     1021 gtatcgcggg ccccggatta cttgcccgcg tgttaacggg aaaatactgc gaacatctgc
     1081 cactgtatcg tcagagtgaa atctttgccc gccagggtgt cgaactgagc cgggccttac
     1141 tctccaactg ggttgacgcg tgctgccagt taatgacacc ggtgaatgat gccctgtacc
     1201 gttatgtgat gaatacccgc aagcttcaca ctgacgacac accggtaaag gtactggcac
     1261 cgggcctgaa aaagacgaaa acagggcgca tctggacgta tgtccgggat gatcgcaatg
     1321 cgggttcgtc atctcctccg gcggtctggt tcgcgtactc atcgaaccgg caggggaaac
     1381 acccggagca acacctccgc cccttccggg gtatcctgca ggcggatgcg ttcacaggtt
     1441 atgacaggct gttcagtgca gaacgtgaag gtagtgcgct gacagaagtt gcgtgctggg
     1501 ctcatgcccg gagaaaaatc cacgatgtat acatcagcag caaaagtacg acggcagaag
     1561 aagctctgaa gcgaatcagt gaactgtacg ccatcgagga tgaaatacgg ggattaccgg
     1621 agtctgaacg tcttgcagcc aggcagcagc gaagtaaagc gttactgacg tcgctgcatg
     1681 aatggatggt ggagaagaat ggcacgttgt cgaaaaaatc cagactgggc gaagcgttca
     1741 gctatgtcct gaatcagtgg gacggcctct gttattacag tgatgacggt ctggcggagg
     1801 tggacaataa cacagcggaa agagcgcttc gtgcagtctg tctcggaaag aaaaacgtgt
     1861 tcttcggtag cgatcacggc ggcgagcgtg gagcactgct gtacgggctg atcggcacct
     1921 gccgtctgaa cggtatcgat ccggaagcgt atctgcgcca tatcctgagc gtactgccgg
     1981 aatggccttc cagccgtgtt gatgaactcc tgctgcatat tagcatggag gtgttcggtg
     2041 agtgccggac gacgtattct ctgtaataat tcttacacct gcgaaatggc atggttcatc
     2101 gtctgacaac ccgggaaaac cgttgaccat ttttatattt caggcacaga tattttcccg
     2161 tcacgaattc atttcccata aggtactggc acctctcctt accggcgagc gttttatctt
     2221 tccctccttt tcgagagtca tcaggtaata tctggcctga taggcactca gcccaaaatg
     2281 tatggcaatt tctgttgtcc ggcacggagt tttcagctcc gtgaggaacg tcagtatatt
     2341 gtctttcata ttctgttaat tcccgtgtag acctgaatat cgtgcatggt atatcaaacc
     2401 atacgaactc ttgtcgttgt taatttcagc catgtaaagc catcagagct ggcagctgac
     2461 gtatataata caacgattat tttctgccgg aagaagaaaa ttccacagtc attttctgtc
     2521 cgcttttgtt tcccccggta atatttttac cctcctgtaa tttcatgtat ttaattaaat
     2581 tatgtgaat
//
LOCUS       DAPHCV010000280         2577 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.285, whole genome shotgun sequence.
ACCESSION   DAPHCV010000280 DAPHCV010000000
VERSION     DAPHCV010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.285"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(6..>2577)
                     /locus_tag="RH033_004810"
     CDS             complement(6..>2577)
                     /locus_tag="RH033_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV2993859.1"
                     /translation="IIKNINNATAINGGLMIGNGSSVYLSGKNSIFNGNISIDEDASM
                     NLSVGNANVHANTITLKSDSWLNIDTSIKNWTQDYYTLLSSDTGISIADNSHIVQYNV
                     LLTEGAESYVYTSLNDDDNKLISMLRWNNTKGMGYGTFNIEKDATLNIGVSLSDNLSP
                     LLYDGWDGKSLTKSGNGTLILSATNNYTGNTEVKSGVLILAAPDALGRTEYLYLSRGA
                     ELDMNGYPQTISKLLTAAGSVLNIHGGSLILNNGGESAGTIAGDGSLNINGGMLDITG
                     NNRNFSGVFTVNKGAHLAVSTADNLGTAFVDNYGTLTLNSTSAWQLTNNISGYGNVRK
                     TGAGALNISDNAKWTGMTDIIQGTVILGNADSPVMLGSNQVIVEEQGKLSGFGGVAGN
                     LSNSGIVDLTTYMPGNILTVGGNYTGRNGLILLQTETGGDNSKTDRLVIKGNASGRTR
                     VAVTQAGGTGAETLNGIEVIHVSGNADNAEFIQTERITAGAYDYILKRGQGINSTNWY
                     LISRKDIPVPQPEAVPESHDNNLRPEAGSYVASIAAANNLFVTNLYERQGQELYISHM
                     TGEENEAGIWMYNKGKHNRWRDNSSQLRTRGNSYVVLIGGDIAQWSLNGTDRWHTGMM
                     AGYGHNNNSTNALSTGYHSEGRMNGYTAGLYATWYANDETHNGSYLDSWLQYSWFDNH
                     INGERLPAESWKSKGFTVSLEAGYSWKAGEFTDNYKGSHEWYVQPQLQVVRMNVKSDK
                     YHESNGTSIENTGNGNILTRLGARTWLTSKNGKNTRYAVPFRPFVEAHWLHNSRVFGT
                     SMNGVSIYQDGARDIGEINGGVVGMITPEVAFRADAGIQLGEHGYHNTSAMLSVEYRF
                     "
BASE COUNT          673 a          647 c          459 g          798 t
ORIGIN      
        1 acacatcaga aacgatattc cacactcaac atggcagatg tattatggta tccatgttct
       61 ccaagttgaa tgcctgcatc agcccggaat gctacttctg gtgttatcat tcccacaaca
      121 ccaccattta tttctccgat atcacgcgca ccatcctggt atatacttac accattcata
      181 ctggtgccga aaacacgact attgtgcaac cagtgtgcct ccacaaatgg tctgaacgga
      241 accgcatacc gcgtattttt accgtttttg ctggtaagcc atgttcttgc tcccaggcgg
      301 gtgagaatat ttccgttacc ggtattttca atactggttc cgttactttc atgatatttg
      361 tctgatttta cattcatccg gacaacctga agctgcggct gaacatacca ttcatgactt
      421 cccttgtaat tgtcggtaaa ctctccagcc ttccatgaat atcctgcttc cagagatacc
      481 gtaaaccctt ttgacttcca tgactcagca ggcagccgtt ctccatttat atgattatca
      541 aaccagctgt attgcagcca gctatcaaga taagaaccat tgtgtgtttc atcattggca
      601 taccatgttg cataaagacc cgctgtgtat ccattcattc ttccttccga atggtatccg
      661 gtgctcaggg cattcgtact gttattatta tgaccatagc cagccatcat acctgtatgc
      721 cagcgatcgg taccattcag gctccactga gctatatctc cccctattaa cacaacgtag
      781 ctattccccc gggttctcag ctgactactg ttgtcacgcc agcgattatg ttttccttta
      841 ttatacatcc agatacctgc ttcattttct tctcctgtca tgtggctgat atacaactcc
      901 tgtccctgtc gttcatacag attcgttaca aacagattat ttgcagcagc aatactggca
      961 acatagctac ctgcctcagg acgcaaatta ttatcatggc tttccggtac agcttcaggt
     1021 tgtggtacag gaatgtcttt tctgctaatc agataccagt tagtgctgtt aatcccctga
     1081 ccacgtttca gtatgtaatc ataagctccg gctgtaatac gttccgtctg aatgaattca
     1141 gcattatcag cattgccact gacgtgaatc acttcaatcc cattaagtgt ctctgcacca
     1201 gtaccaccgg cctgagtaac agcgacacgg gtacggccac tggcattacc tttaatcacc
     1261 agacgatctg ttttcgaatt gtcaccacct gtttctgtct ggaggagaat aagtccattt
     1321 ctgccagtgt aattccctcc gacagtcagt atattacccg gcatatatgt agtgagatcg
     1381 actataccac tattgctcag atttcctgca acgcccccaa acccggagag tttgccctgc
     1441 tcttcaacaa tgacctggtt actgccgagc atcaccggtg aatctgcgtt ccccagtatc
     1501 actgtcccct gaataatatc tgtcatcccg gtccattttg cgttatcgct aatgttcagt
     1561 gcacctgctc ccgtcttgcg aacattacca taaccactga tattgttggt aagctgccat
     1621 gctgatgtac tgttcagagt taatgtgcca tagttatcaa caaaggctgt ccccagatta
     1681 tcagccgtgg atacagccaa atgagccccc ttattcacgg taaaaacacc ggaaaaatta
     1741 cgattattac ccgttatatc aagcattccc ccatttatgt tcagagaacc atcccctgca
     1801 atagtacctg cagattctcc tccattattc agtatcagac ttccgccatg aatgttcagc
     1861 acagagcctg cagccgtcag tagtttgctt attgtctgag gatacccatt catatccagt
     1921 tctgcgccac gggataaata taaatactca gtccgaccaa gagcatcagg tgcagcaaga
     1981 attaatacgc cagatttaac ctctgtattt cctgtataat tgtttgttgc agaaagtata
     2041 agagtaccat tacctgattt tgtcagactt ttgccgtccc agccatcata taataaaggt
     2101 gaaagattat cggaaagaga aacgcctatg ttcagagtgg catctttttc tatattaaag
     2161 gttccatatc ccatcccttt tgtattattc catctcagca tggatatcag tttgttatcg
     2221 tcgtcattta aagacgtata aacataactt tcagcacctt ctgtcagtaa tacattgtat
     2281 tgtacaatgt gactattatc agcgatcgag atacctgtgt ctgacgacaa taatgtgtaa
     2341 tagtcctgag tccagttctt aattgatgtg tctatattaa gccatgaatc actttttaat
     2401 gtaatagtat ttgcgtgaac attagcattt cctacagaca ggttcataga tgcgtcttca
     2461 tcaatactta tatttccgtt aaaaatggag ttcttgcctg acagataaac agatgagcca
     2521 ttcccaatca ttagcccacc attaattgct gttgcgttat tgatgttttt aataatt
//
LOCUS       DAPHCV010000281         2536 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.286, whole genome shotgun sequence.
ACCESSION   DAPHCV010000281 DAPHCV010000000
VERSION     DAPHCV010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.286"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(188..1600)
                     /gene="pykF"
                     /locus_tag="RH033_004811"
     CDS             complement(188..1600)
                     /gene="pykF"
                     /locus_tag="RH033_004811"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HDV2993860.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            2157..2366
                     /gene="fumD"
                     /locus_tag="RH033_004812"
     CDS             2157..2366
                     /gene="fumD"
                     /locus_tag="RH033_004812"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HDV2993861.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          680 a          531 c          608 g          717 t
ORIGIN      
        1 catcgaacgc tgggttttag ataaaaaaat tgagagaaaa caggatgctt ccatcggatt
       61 catcttagat aaagtgagat tatatagtga aatccgattt gattttttat tgcttctggt
      121 tatcgattaa ataaaaaaag cgcccatcag ggcgcttcga tatacaaatt aattcacaaa
      181 agcaatatta caggacgtga acagatgcgg tgttagtagt gccgctcggt accagtgcac
      241 cagaaaccat aactacgaca tcacctttgt gtgccagacc gctctgcaga gccagttctt
      301 tacccagacg gtagaagtca tcagtagaag tgatctcttt aaccagctgc ggcacaacgc
      361 ctttgctcag tactaactgg tgagctgttt tttcgttggt ggtcagtgcc aggatggtgg
      421 catccgggaa gtatttacgt actgcgcgag cagatttacc gccctgagta gcaaccacga
      481 tcagtggagc atccagtttt tcagcagttt caacggcacc acggcatacc gcttcggtaa
      541 tacgcagttt acggttgtca ttgttgaact cgagacggct gttcatcacg cggtctgtac
      601 gttcgcagat ggtcgccatg atagaaaccg cttccagcgg gtatttacct tttgcggatt
      661 caccagacag catcactgcg tcagtaccgt cgaggatggc gtttgcaacg tcaccggctt
      721 ctgcgcgagt cgggcgtggg tttttgatca tggaatccag catctgggtc gcagtgataa
      781 caactttacg tgcacggata catttttcga tcatcatctt ctgggcgaag ataacttctt
      841 ctaccgggat ttctacaccc aggtcgccac gcgcaaccat gatgccgtca gaggcttcga
      901 ggatttcgtc gaagttgttg aggccttcct ggttttcgat tttggagatg atgtggatgt
      961 tttcgccgcc gtgcgctttc aggtgctcac ggatttcgat aacgtcagaa cgcttacgaa
     1021 taaaggaagc agcaacaaag tctacgcctt gttcgcaacc aaagatcagg tcctgtttgt
     1081 ctttttcagc cagtgctggc agggcaatgg aaacgccagg caggttcaca cctttgtttt
     1141 cgcccaggtc gccgttgttc agcactttac agataacttt gttaccttca atagcggtaa
     1201 cttccatacc gatcagaccg tcgtcaacca gtacggtgtt gccaacagac aggtcagtag
     1261 tgaaaccttc atacgttacc gcaaccattt cgctgttgcc gataacagat ttatcagtag
     1321 tgaaagtaaa ggtctgaccc gctttcagag aaacgtcgtt accgccttcc agtttcatgg
     1381 tgcggatttc cggacctttg gtatcaagca ggatagcggc ggttttacca gttttgctca
     1441 tcacgttgcg cagattctga atgcgctgac cgtgttctgc atagtcacca tgagagaagt
     1501 tcagacgcat aacgttcatg ccagcgtcca gcattttagc taacatctct tcagattcgg
     1561 ttttcggtcc gatggtgcaa acaattttgg tctttttcat gacagtctta gtctttaagt
     1621 tgagaaggat gggagaaact tgctttctgg gcgcactgcc ggaaagcgaa tccggtatta
     1681 cgaaagtggt ggtgcctcgc tctaaggata ggtgacatcg aaaaagcgtg cagaggaatg
     1741 tgtgcttgtg tctcagccca acgtgagtgt ctgtttttga gtcgttgagt tctacagtcc
     1801 aaagtgctga aaccattcaa gagtcaattg gcgcgcatta tacgctgaaa ttaatccaaa
     1861 aggaaaacaa aaacagcgtt tcaaaaaata tacaacttgt tagacaataa attgttatca
     1921 aagatttaac gttccaggga aaaggttacg caaaaacttc actgtttgcg caacgtctgg
     1981 tgccggggtt gtcagtgtga ggataagtga aagttcatcc ggtgtataaa tacatcatcg
     2041 ctcaattgct acagttaaaa taatttatca cgttatttgt ctgattttgt ttgatttttg
     2101 tttaattggt tagattgata tatgctgata gttggcatga taaataggta taaagaatgg
     2161 gaaaccggac aaaagaggat gaactgtacc gagaaatgtg cagagttgtc ggtaaagttg
     2221 tgctggaaat gcgcgatctg gggcaggaac caaagcatat tgttatcgca ggcgtattac
     2281 gtacagcatt agccaataaa cgcattcagc gcagtgagct ggaaaagcag gcgatggaaa
     2341 cagtcattaa cgcactggta aaatgaccat tcaggatgag gcggtaagct tcatcctgat
     2401 attgttagtt cagttgcggt ccagttaatc attctaaata acaactgctg aaattatacg
     2461 aatttcggtg tatattttaa tttatagcga taaagttatt tttattttct gcgatgaact
     2521 tcacaaataa ttgtcg
//
LOCUS       DAPHCV010000282         2503 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.287, whole genome shotgun sequence.
ACCESSION   DAPHCV010000282 DAPHCV010000000
VERSION     DAPHCV010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.287"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(12..509)
                     /gene="rrrD"
                     /locus_tag="RH033_004813"
     CDS             complement(12..509)
                     /gene="rrrD"
                     /locus_tag="RH033_004813"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HDV2993862.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVRHNPYKD
                     IVGVWTVCHGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREIC
                     LWGQQ"
     gene            complement(509..724)
                     /gene="essD"
                     /locus_tag="RH033_004814"
     CDS             complement(509..724)
                     /gene="essD"
                     /locus_tag="RH033_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HDV2993863.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGG"
     gene            1297..2394
                     /gene="ompC"
                     /locus_tag="RH033_004815"
     CDS             1297..2394
                     /gene="ompC"
                     /locus_tag="RH033_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HDV2993864.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYAHLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTQRATGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRSDFDNYTEGNGDGFGFSATYEYEGFGIGATYAKSDRT
                     DTQVNAGKVLPEVFASGKNAEVWAAGLKYDANNIYLATTYSETQNMTVFADHFVANKA
                     QNFEAVAQYQFDFGLRPSVAYLQSKGKDLGVWGDQDLVKYVDVGATYYFNKNMSTFVD
                     YKINLLDKNDFTKALGVSTDDIVAVGLVYQF"
BASE COUNT          739 a          564 c          514 g          686 t
ORIGIN      
        1 ggttactctg ttcattgctg accccacaaa cagatttcac gctcaatctc acgacgagtc
       61 atgagacctt tccattgctt accgccagca tatgtccagc gacgtagctg atcacatgcg
      121 cctttaatat caccctggtt tattttgcga agaagcgtcg atgttctgaa attgccagca
      181 cccacgttgt agacgaacga gtaaagagcg ccgcgcgttg tttccggtat atcgactttg
      241 atgtacgggt taatttgtct ggcgacagtg gcaaggtctt tattcaggag ggctttgcac
      301 tctgcttcgg tatacgtttt accgggaatg atgtcttttc ctgtatgccc gtgacataca
      361 gtccatacac caactatgtc tttgtaagga ttatgtctca caccttccag accatcgtta
      421 ccacttgggc cagtgattaa tacagatgct atagcaatag ccccgccacc aatagcagca
      481 gcaacagctt ttcgtaatga tggaggcatt atccacctct cgcagccttg cgcttatctt
      541 ctttaatctt gaaataaagg tttgtcaggt acgtcagcag gccaaatacc aggctaccca
      601 gcactccaat tgccgcccac tgtgagggcg tgactttatc tagcagctgt aaaaaccagt
      661 acccggcact acctgctgag gtgccatagg cgacacccgt tgttaactta tccatggatt
      721 tcataacccc acctcgcaga caaagcgggt gtaaattgag ggaatactac gaaacgtaac
      781 agactcggag tcagtgaata actcaggtat tgggttatca gctaatatcg agactcaaaa
      841 aatggaaaaa cccgctcgac ggcgggttta agctgtgtga cgaagtaacc actcttaaca
      901 gcataaccaa ttttttacgt acgtaaacca ctaaatgata tttgcgagaa tgctaccgag
      961 tattgaaaac accactacaa atacataagc aaatctcaac aaataaccaa caaataattt
     1021 ccagtgttat ttttagctgg tttaaattga accttcaaat tatagagcac ttataaataa
     1081 cagccgttaa tataaattgg ctaacagatt tatttttatt cagccaagag ccatgaatag
     1141 gattcgatag aaaaaagttc agataaaaat agagatctac ttcacaaatc aaacgagaaa
     1201 ccaaaactta catcttgaaa taatcacatt gattagatga atatttatcg cgcagtgaca
     1261 tcatttttta ataatagttc aaaaaaaggg ctcacgatga aaaaattaac agtggcaatt
     1321 tctgctgtag ctgcatcagt actgatggcg atgtctgctc aggcagctga aatttataat
     1381 aaagacagta acaagctgga tctgtacggg aaagttaatg ctaagcacta cttctcctct
     1441 aatgatgcag atgatggtga tactacttat gcccatcttg gcttcaaagg tgaaacccaa
     1501 atcaacgatc aactgactgg tttcggtcag tgggaatatg aattcaaagg caaccgcgct
     1561 gaatctcaag gttcctccaa agataaaacc cgtcttgcct tcgctggcct gaaattcggt
     1621 gactacggct ccatcgatta cggccgtaac tacggtgtag catacgacat cggtgcgtgg
     1681 actgacgtcc tgccagaatt cggtggtgac acttggactc aaaccgacgt gttcatgact
     1741 caacgtgcaa ctggtgttgc aacctatcgt aacaacgact tctttggtct ggttgatggt
     1801 ctgaattttg ctgctcagta ccaaggcaaa aacgatcgta gcgatttcga taactacact
     1861 gaaggtaacg gtgatggctt cggtttctct gctacctatg aatacgaagg attcggtatc
     1921 ggtgcaactt atgcgaaatc tgatcgtacc gacactcaag ttaatgcagg gaaagttctt
     1981 cctgaagtat ttgcttccgg taaaaatgca gaagtttggg ccgcaggtct gaaatatgac
     2041 gctaacaaca tttacctggc cactacctat tctgaaaccc agaatatgac tgtatttgct
     2101 gatcacttcg ttgctaataa agcccaaaac ttcgaagctg ttgcacaata tcagttcgat
     2161 ttcggtctgc gtccgtccgt tgcttacctg caatctaaag gtaaggatct tggagtatgg
     2221 ggcgatcagg acttagtcaa atatgttgat gtaggtgcaa cctattactt caacaaaaat
     2281 atgtctactt tcgttgatta caaaatcaac ctgcttgaca aaaatgactt cactaaagca
     2341 ctcggtgtaa gcactgatga catcgttgct gtaggtctgg tttaccagtt ctaatctgat
     2401 tacgaaaaag atatgttgcg ggaggcgttg cctccccaac atataagtgg ctccctcaag
     2461 ccacttcctt tagaagcaca accttgcttc taactatata aac
//
LOCUS       DAPHCV010000283         2484 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.288, whole genome shotgun sequence.
ACCESSION   DAPHCV010000283 DAPHCV010000000
VERSION     DAPHCV010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.288"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..315
                     /locus_tag="RH033_004816"
     CDS             <1..315
                     /locus_tag="RH033_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
                     /protein_id="HDV2993865.1"
                     /translation="LGGTFKYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNA
                     GYYVTPNAKVYVEGAWNRVTNKKGDTSLYNHNDNTSEYSKNGAGIENYNFITTAGLKY
                     TF"
     gene            500..1984
                     /locus_tag="RH033_004817"
     CDS             500..1984
                     /locus_tag="RH033_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV2993866.1"
                     /translation="MRKIIAHFKIVLTLLLPVTVSAQQIEWQSCMTSQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCQQSDTEIQENITEKQRVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHDLLLKLHHRPLIDSKGENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALNAPIASEESSDASGVILCVDQGDEQLTPEERKSRKGALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHLITPQKIENIYCPGNSEAEIQ"
     gene            complement(2167..2472)
                     /locus_tag="RH033_004818"
     CDS             complement(2167..2472)
                     /locus_tag="RH033_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDV2993867.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVIIQFYIQEFSEYIAPLQD
                     AVDLEIATEEERSLLEAWNKYRVLLNRVDTSVALGMKLIVTHVTVTVIAISKVQNVY"
BASE COUNT          784 a          543 c          477 g          680 t
ORIGIN      
        1 ctaggtggca catttaaata cagcggctgg gtggaagcat ctgataacga tgaacactat
       61 gacccaggaa aaagaatcac ttatcgcagt aaagtcaaag accaaaatta ctattctgtt
      121 gcagtcaatg caggttatta cgtaacgcct aacgcaaaag tttatgttga aggcgcatgg
      181 aatcgggtta cgaataagaa aggtgatact tcactttata accataatga taatacttct
      241 gaatacagca aaaatggtgc aggcatagaa aactataatt tcatcactac tgctggtctt
      301 aagtacacct tttaacaccg ttaactaaaa tctccccggt ggtaatgcat agccccgggg
      361 agtttatctg ctattcaaag taaacaacat tctctaattt tgtactcatt tgaccaatat
      421 ccgtaacgtg tagcccccta ccgcaacaca gggggcaata tattcaaatt ttatatttat
      481 actgatattc agaggcacaa tgcgaaagat aattgctcat ttcaagattg ttttaacctt
      541 acttctaccc gtaaccgtat ctgcccagca gatcgagtgg caatcctgta tgaccagtca
      601 attcaaccac tggtttggtg aggaaaaacc gtctcctgac ttactatgtg gttatttgtc
      661 tgttccatta aaatatacag acacaggcgg agatgcttct tatgaaaaaa aatcacaagt
      721 caaactagcg ttgacaaaat tgccagcaaa aagcaagcat aaaggaagca tcctgataat
      781 aagtggtggg cccgggttac caggcataaa tccttatatt aactttgact ggccagtcac
      841 aaatctgcgt gagtcatggg atattattgg atttgatcct cgaggcgtcg gacagtccac
      901 tccgacaata aactgccagc aatcagatac agagattcag gaaaacataa ccgaaaagca
      961 acgagtatta aataaaatta atgcatgtat ccataatacc ggagccgaag tcattcgcca
     1021 tataggctct aacgaggctg tatatgatat tgatcgtatt aggcaagcct tgggtgataa
     1081 acaactgact gccgtggcgt attcgtatgg aacacaaatt gcagccttat atgcagaacg
     1141 ttttccttac aacgtcagat ctatcgttct ggatggagtc gtcgatattg atgacctgga
     1201 ggacaacttc acatggcaac tcaaacaggc acaaagttat caggaaacgt ttgatcgctt
     1261 tgcatcctgg tgtgcgcgta caaaaagttg cccgctttct tcagacagag ataaggcaat
     1321 aactcagttc cacgacctgt tattaaaatt gcatcacaga cctttaatcg acagcaaggg
     1381 agaaaatata tcttcagatg aactcatatc attaacaaca gaccttctat tatggcgttc
     1441 atcatggcca acccttgcaa ctgccatacg ccagttctct caggggattg tcagtaatga
     1501 aattgaaact gcgctcaatg ctccgatagc ctcagaagag tcaagtgatg cttcgggggt
     1561 aatcctttgt gtagatcagg gggatgagca attaacacca gaagagcgaa aatcccgaaa
     1621 aggcgctctt gcgaatgcct tcccggctat taactttgac aatggacgtt ccgattcacc
     1681 tgatttttgt gaattatggc caatacatag cgacctgaac aaaactcgcc tgaaaaatac
     1741 tgttctgccc tctggtttac tgtttgtagc acacaaatac gacccaacaa cgccctggat
     1801 taatgcccgt aagatggcag agaaattttc cagcccgtta ctaacaataa atggtgatgg
     1861 gcatacatta gctctcaccg gagttaattt atgtgtagat aaagcagttg tacatcacct
     1921 gatcactcca caaaaaatag aaaatatata ctgcccagga aattctgaag cagaaataca
     1981 ataattacaa aatattccgc tatttgctcc caatgcaaaa catatattgc attgggagca
     2041 tattcatatt tttgttattc ttgtatgaaa tcgtctcatg tacacggtct tttttcgccg
     2101 aaaactgata ttactttaat caaaagttga cgtgccagat tcgaagtaaa tttataaaat
     2161 atcaaatcaa tatacatttt gcaccttcga gatagcaatg accgttactg taacatgagt
     2221 tacaatcagc ttcataccca gagctactga tgtatcaaca cgattcagca atacccgata
     2281 tttattccat gcctccagca acgatctttc ttcctccgtt gcgatttcca gatctacagc
     2341 atcctgcagt ggcgcaatat actcactgaa ctcctggatg tagaactgta tgatgacggt
     2401 cttccagcca ttcggctcct gctgtatcga agcataccag gctatttcaa tatcgctatg
     2461 ctgcggcagc atttaacccc ttgt
//
LOCUS       DAPHCV010000284         2439 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.289, whole genome shotgun sequence.
ACCESSION   DAPHCV010000284 DAPHCV010000000
VERSION     DAPHCV010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.289"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            247..489
                     /locus_tag="RH033_004819"
     CDS             247..489
                     /locus_tag="RH033_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HDV2993868.1"
                     /translation="MTNKHLSLFSPFMLTEKIKPCNRIVMAPMTTWSANPDGTISEQE
                     LEYYKRRSQNIGLVITGYTYVTPRKFRHRKELAGFR"
     gene            970..1071
                     /locus_tag="RH033_004820"
     CDS             970..1071
                     /locus_tag="RH033_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993869.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            1435..1698
                     /locus_tag="RH033_004821"
     CDS             1435..1698
                     /locus_tag="RH033_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDV2993870.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            1698..1838
                     /gene="ykgO"
                     /locus_tag="RH033_004822"
     CDS             1698..1838
                     /gene="ykgO"
                     /locus_tag="RH033_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HDV2993871.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(1908..2098)
                     /locus_tag="RH033_004823"
                     /pseudo
     CDS             complement(1908..2098)
                     /locus_tag="RH033_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          752 a          526 c          453 g          708 t
ORIGIN      
        1 attctaaact ttcaggagat ttattatgaa taaacgtacc ctcacgattg tgccattctt
       61 cttaccgata ttctgacaca catcattaac tttctatttt tactcttttc tccaccataa
      121 aatcaataaa agcactaaca gtaattataa ataaaaacaa agccaaaaat cttggttcca
      181 aagctggtaa caactaacca gtttaaatat acacatcaaa atcagataaa catttgaggt
      241 attgtcatga caaataaaca cctatcactg ttttctcctt ttatgctgac agaaaaaata
      301 aagccatgta atcgtatcgt tatggctcct atgacgacct ggtcagctaa tcctgatgga
      361 accatctctg agcaagaact cgaatattat aaacgtcgct cacaaaatat tggtttagta
      421 attacaggat atacctatgt cacgccaagg aaattcaggc atcgaaagga actggctggt
      481 ttccgttaat agattaaata tctattactc tcccgagagt aaaactatca aatcaatgag
      541 taaccgatac agcctcgcaa attctttcag ataattaacc cgtcactgcg gcacgttctc
      601 tcgtaaggcg tgcctgctgt gtaatccagt atgtatccgg catagttgac ggtttaatag
      661 aactcaattc ctctcccagg tcaccaacaa cgatatcttg tataacaccc caggagatac
      721 gacattatga tgagatgagg tatgctcctc agcgccaatc aacgccaggg ctgggccagt
      781 tttgagatca gactcattcc cggactgact tcaggcgaca tatatcaatg acgttggggc
      841 aataaaagat agccgatgta atacatctat tcaaatttgt gatatatgtc aaaaaattgg
      901 ttgaccaaac tcgttattta tataagggca cttacgaagt gcactctttt ttaaagcgag
      961 gaagtaccaa tgaaagagaa taaagtacag caaatcagtc ataaactgat taatatcgtt
     1021 gtttttgtcg caattgtaga atacgcctat ttatttctcc atttctatta ataacggcaa
     1081 taaactgttc acttcagtga tatttaaaat atgcatcctc tccctttttt gtaagtaatt
     1141 attatatccg tgggagagga atatacgctg tcaggtaatc aatcatgctg caataaatca
     1201 tcggccagta aagtggagat agcctccatt ctcgaaaaat ccatactctc agcgaaacca
     1261 tcatcaatca ctcatccagg cgtttatggg agcgtcgcca atggctgcta acaatgccag
     1321 acttccccgt tgcggaaatt ccacatccca caaatagtca cagtgattgg gtgttgaaat
     1381 gatccggatg agcatgtatc tttacggtta tgttataaca taacaggtaa aataatgaaa
     1441 cccaatatcc atcctgagta tcgtactgtg gtgttccacg acaccagcgt tgatgagtac
     1501 tttaaaatcg gctcgactat caaaacagac cgtgagattg agctagatgg cgtaacgtat
     1561 ccatatgtga caattgatgt ctcttctaaa tcgcacccgt tctatacagg gaagctgaga
     1621 acagtagcat cagaaggaaa tgttgcacgg ttcacccaac gttttggtcg ttttgttagc
     1681 acgaaaaagg gggcatgatg aaagtcctta actctctgcg taccgcaaaa gaacgccatc
     1741 cagactgtca gattgtgaag cgaaaaggac ggctatatgt gatttgtaaa tctaatccac
     1801 gttttaaggc cgttcagggt cgtaagaaaa aacgttgatt caaaattcga cgaattaacg
     1861 atatttgtct gattaataat cagaccggat taatgttggt gtatttatta caccagcatt
     1921 aattttcctg gggatgtatc ttcctgttca tttaaggtca actgcctgac gtttctctcc
     1981 gaatattcca ttatattaat gttgacttgt tgaccatttt cgccctgtat gctggcatca
     2041 actctatttt agactgaaca cgccactcag tttcctccct ttgcgacgca gcctgcattt
     2101 acactcaaac tgttaagatg ataaatgtgg taaatctgtt ggtactgaca taaaaacgtt
     2161 tacgccacag gaacggccag atccatcggt aaccccatcg ccgacgttcg agtgccagtt
     2221 agagtaacac gcacagatga ctgaatgcag tgccctggca aagaggccat cgttcctgtg
     2281 acaactggca gtcttcgttt aacttcactt aatttggctc ttggggggct taccggacag
     2341 atgacgtact tacacctgtt taatttttca tcacttattg ggatgaacac ccataaccat
     2401 tttgtgcggc atgttaatcc attaaaacac cttaccgat
//
LOCUS       DAPHCV010000285         2387 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.290, whole genome shotgun sequence.
ACCESSION   DAPHCV010000285 DAPHCV010000000
VERSION     DAPHCV010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.290"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            6..1154
                     /locus_tag="RH033_004824"
     CDS             6..1154
                     /locus_tag="RH033_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012769665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate lyase"
                     /protein_id="HDV2993872.1"
                     /translation="MKFESFYDSESNNEVIRLTPTDILCHRNYFYQKCFTNDGKKLIF
                     AAEFDCNRNYYLMDLVTQTAKQLTEGAGDNTFGGFLSPDDNYLFYVKNEKHLQRVDLT
                     TLEEVTIYTVPDNWVGYGTWVANTDCTEIVGIEISKTDWTPLSDWKIFLEFYHKNPRC
                     RLITIDLETGNAEVILDRNTWLGHPIFRPNNNDTIAFCHEGPHDLVDARMWMINRDGT
                     NERKVHEHQLGESCTHEFWIPDGSALAYVSYMKGQQQRYIRKYNPDTDEDSLVMEMPA
                     CSHLMSNHNGILYVGDGSGSPVDVKDTCSYKIENDPWIYILSSKNKKIHKLVKHNSSW
                     RVQKGDRQVTHPHPSFSPDNKYVLYSCDAEGEPALYMAKIPEEILTQQ"
     gene            1184..1984
                     /locus_tag="RH033_004825"
     CDS             1184..1984
                     /locus_tag="RH033_004825"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HDV2993873.1"
                     /translation="MDFINMFSLKGKNALVTGASYGIGFEIAKALSNAGATIIFNDVI
                     EENIKKGLAAYKENNINAHGYLFDITDEQSVKENIKKIESDVGIIDILVNNAGIIQRK
                     PMLETTAADYRKIIDIDLTGQFIMAKAVLPSMIKKGHGKIINICSMMSELGRETVVGY
                     ASAKGGLKMLTKNICSEFGEKNIQCNGIGPGYIATPQTAPLREIQPDGSRHPFDQFII
                     SKTPAGRWGKPDDLAGAAIFLASDASNFINGHILYVDGGILAYIGKQP"
     gene            2039..2362
                     /locus_tag="RH033_004826"
     CDS             2039..2362
                     /locus_tag="RH033_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDV2993874.1"
                     /translation="MFVFNEDTAIEDLGNGVKRKVLAHGGNMMAVEVHFSKGAIGSLH
                     HHQHEQLTYVLSGIFEFTIGDEVRTVKTGDTLYKQPNIVHGCKCLEKGVLLDIFTPQR
                     QDFLK"
BASE COUNT          769 a          401 c          506 g          711 t
ORIGIN      
        1 aaaagttgaa gtttgaatct ttctacgata gtgaatcgaa taacgaagtt attcgattaa
       61 cgccaacaga tattctttgc catcgcaatt atttttatca aaaatgtttt acaaatgatg
      121 gtaaaaaatt gattttcgct gctgagtttg attgtaaccg gaattattat cttatggatt
      181 tggtaactca gacagcgaaa caactcactg aaggagctgg agataacacc tttggtggct
      241 tcctgtcccc tgatgataat tatctttttt atgtgaagaa tgaaaaacat ttgcagcgtg
      301 ttgatttgac cacattagaa gaagttacca tatacacagt accggataat tgggtggggt
      361 acggaacatg ggttgctaat actgattgta cggaaatcgt cggaatcgaa atcagtaaaa
      421 ctgactggac ccctcttagc gactggaaaa tatttctgga attttatcat aagaatccac
      481 gatgccgttt aattacgatt gatctggaaa ccggaaacgc tgaagtgata ttggatcgta
      541 atacttggtt agggcatcca atttttcgtc cgaataataa cgatactatc gccttttgcc
      601 acgaagggcc ccatgacctg gtggatgcaa gaatgtggat gattaaccgt gatgggacaa
      661 atgagcgtaa agtgcatgag caccaacttg gtgaaagttg tacacacgag ttctggatcc
      721 ctgatggatc tgcactggct tatgtttcct atatgaaagg acagcaacag cgttatattc
      781 gcaaatataa tcctgatact gatgaagatt cattagtgat ggaaatgccg gcatgttccc
      841 atttaatgag taaccataat ggtattctct atgttggtga tggttcaggt agtccggttg
      901 atgttaaaga tacctgcagt tataaaattg aaaatgatcc ctggatttac attcttagca
      961 gtaaaaataa aaaaatacat aaattggtga aacataatag ttcctggagg gtacagaaag
     1021 gagaccggca ggtaacgcac ccgcatccat cattttctcc agataataag tacgttttat
     1081 attcttgcga tgccgaaggt gaaccagcac tatatatggc taaaattcct gaggaaatct
     1141 taacccagca ataactgaca aatttttcac tattggagtt tttatggatt tcatcaatat
     1201 gttctctctt aaaggtaaaa atgctttagt cactggagca tcatatggta ttggttttga
     1261 gatcgctaaa gcactaagta acgctggtgc aacgatcatt tttaatgatg taatagaaga
     1321 aaacattaaa aaaggcctgg cagcatataa agagaacaac atcaacgcac atggttattt
     1381 atttgatatt acagatgaac agtctgtgaa ggaaaatatc aaaaaaatag agagtgacgt
     1441 aggcattatt gatattctgg taaataatgc agggattatt cagcgtaaac ctatgctaga
     1501 aactacagca gctgattatc gcaaaataat agatattgat ctgacaggtc agtttattat
     1561 ggctaaggcg gtattgcctt caatgattaa aaaaggacat ggcaagatca ttaatatctg
     1621 ttccatgatg agcgaattgg gtcgcgaaac cgttgtaggc tacgcttcgg caaaaggcgg
     1681 cttaaaaatg ctgactaaaa atatctgttc tgagtttggt gaaaaaaata ttcagtgtaa
     1741 tggtattggt ccaggctaca ttgctacacc tcaaacagca ccgttacgtg aaattcaacc
     1801 agatggcagc cgccatccat ttgatcagtt tattatatca aaaacgccag ctggacgctg
     1861 gggtaaacca gatgatcttg ctggtgcagc tatattttta gcatcggatg cttcaaactt
     1921 tatcaatggt catattttat atgtcgatgg tggcattctg gcctacattg gcaaacagcc
     1981 ttagttatat tcagtcataa ctgtatgtac tactttaaat attttcagag aggtggtgat
     2041 gtttgtattt aatgaagata cagcaataga agatttaggc aacggggtca aaagaaaagt
     2101 tttggctcat ggtggcaata tgatggcagt agaggtccat ttctctaagg gggctatcgg
     2161 ctctttacat caccaccaac atgaacagtt gacgtatgtc ttatcaggta tttttgaatt
     2221 taccatcggc gatgaagtgc gtacagtaaa aactggtgat actttatata agcagcccaa
     2281 cattgtgcac gggtgcaaat gtctagaaaa aggagttttg cttgatatat ttacacctca
     2341 acgtcaggat tttttaaagt aaatacccag tattaatatg aactaaa
//
LOCUS       DAPHCV010000286         2369 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.291, whole genome shotgun sequence.
ACCESSION   DAPHCV010000286 DAPHCV010000000
VERSION     DAPHCV010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.291"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            151..245
                     /locus_tag="RH033_004827"
     tRNA            151..245
                     /locus_tag="RH033_004827"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:185..187,aa:Sec,seq:tca)
     gene            298..1335
                     /locus_tag="RH033_004828"
     CDS             298..1335
                     /locus_tag="RH033_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HDV2993875.1"
                     /translation="MSDKDLTQPPAGEFIMFASGDGRVRVECRFESDTIWLSQAAMAE
                     LYDKDVRTINEHLINIFSEGELVQNSTIRKFRIVRQEGKRQVSREIDHYNLEAILAVG
                     YRVRSPRGTQFRQWATQTLQEYLIKGFVMDDERLKNPPVGSSVVPDYFDEMLERIRDI
                     RASERRVYLRVREIFALAADYQPSLKETTQFFQTIQNKLHFACTGHTAAELIHKRADA
                     SQPHMGLTSYKGEEVRKGDVTVAKNYLTQDEVSELNRVVNMWLDFAEDQARRRQQVFL
                     RDWQDKLDQFLQFNDREVLQGAGKISKKMADEKAQAEYVQFAEQQRRLKEAEGEKDIA
                     GLLQWNKESKK"
     gene            1905..>2369
                     /locus_tag="RH033_004829"
     CDS             1905..>2369
                     /locus_tag="RH033_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020237521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993876.1"
                     /translation="MGHGTCKAAKSARHLIESKEKNRSSRVKVFSTHINLSHPAASWL
                     ATQTCANNIFNLLQNNNAIYLFENDNKIELRMGYDSFFSIETIDKINKENIKEIKLKN
                     DTTYVSLPNPTILITTTSTPERQFSISVLDDDNKENLPTDTAPCFTIGIPDEY"
BASE COUNT          709 a          531 c          514 g          615 t
ORIGIN      
        1 atatcattgt aaaatatgag ttttatatga actataatgt tttcgtgata atacgctgcg
       61 tgtattaggc ggaaaaaact gatctggggg atgtagaaac tcaaggaagt agctataatg
      121 cgccccgcct ccatgtagca atcgaggcgc ggaagatcgt cgtctccggt gaggcggctg
      181 gacttcaaat ccagttgggg ccgccagcgg tcccgggcag gttcgactcc tgtgatcttc
      241 cgccaaaatg cctctcctga actctcccga cgtcaacaac actccttcgg aggcgacatg
      301 tcagacaaag acttaaccca gcccccagca ggcgagttta ttatgtttgc cagcggtgac
      361 ggaagagtgc gtgttgaatg tcgctttgag tcagacacaa tctggttatc tcaggcggca
      421 atggctgaac tgtacgataa agatgttcgc actattaacg aacatttaat caatatattt
      481 tccgaaggtg aacttgttca aaactcaacc atccggaaat tccggatagt tcgccaggaa
      541 ggaaaacgtc aggtttccag agagatagac cattacaacc tcgaagccat tctcgctgtc
      601 ggctatcgcg ttcgctcgcc tcgaggcacg caattccgcc agtgggccac ccagacgctt
      661 caggaatacc tgatcaaagg ttttgtgatg gacgatgagc ggctgaagaa tccgcctgtc
      721 ggttcatcgg ttgtgcccga ctattttgat gagatgcttg aacgcatccg cgatattcgc
      781 gccagcgagc gtcgggtata tttgcgggtg cgggagatct ttgctttagc tgccgactat
      841 cagccatcgc ttaaagaaac cacgcaattt tttcaaacca tccagaataa actgcatttt
      901 gcctgtaccg ggcataccgc tgcggaactc atccacaaac gagcagatgc cagccagccg
      961 cacatgggct tgaccagtta taaaggtgaa gaggtacgta agggcgacgt gacggtggca
     1021 aaaaattatc tcacccagga tgaggtcagc gaacttaatc gcgtggttaa tatgtggctg
     1081 gactttgccg aggatcaggc ccgtcgtcgt cagcaagtct ttttgcgcga ctggcaggat
     1141 aagctggatc agttcctgca atttaacgac cgagaggttc tacagggcgc tggtaaaatc
     1201 agcaagaaaa tggccgatga aaaagcgcag gcggagtatg ttcagtttgc cgaacaacaa
     1261 cggcgcttaa aagaagccga aggcgagaag gatatcgctg gtttgctaca gtggaataaa
     1321 gagtcgaaaa agtagcccgg aatcccccca aacgccatac gcaaaaccat ttgggggtac
     1381 ttatggggtt actcatttat caagttcaaa atataaacat taaatcaata acttaaccat
     1441 caaatgcgac tcctgtgatc gccaaaatgc ctcttcagtg atccccatcc tgcttatttc
     1501 tgagcaacct gaccctgctg atgtatctgg tagtatgaca gagtgattcg tttttcttgc
     1561 cgagtgtacg aaaacgtctt ccttgccacc gactatcaac cgtcactcaa aaaaacctct
     1621 ctttttttca agctatcaag aataagttgc attttatctg caccggacac cagccatcca
     1681 tatatggctt ttacctgcta caaagatgaa gaagtgcata aatgtgatgt taaaacaatc
     1741 aaagaaaaat tacatgcgca ataatttcac tataacatta taatccatgg tgaaaataac
     1801 actgcacaag gattgtcatc atcaaactca tcggaactat tttttctgtc gtcattggtg
     1861 taatactgat tgtaagtatc ctttcaggag attatttttg ccgtgtgggg catggaactt
     1921 gtaaagcagc caaaagcgct aggcatttaa ttgaaagtaa agagaaaaac agaagtagtc
     1981 gcgtcaaagt ctttagcacg catattaatt tgagtcatcc agcggcatcc tggctagcaa
     2041 ctcaaacttg cgctaacaat atattcaatc tgcttcaaaa caataatgcc atttatttat
     2101 tcgaaaacga taataaaatt gaactgcgaa tggggtatga ttcttttttc tctattgaaa
     2161 cgattgataa aattaacaaa gaaaacatca aagaaataaa actaaaaaat gatacaacat
     2221 atgtttctct tcctaatcca accattttaa taaccacgac atcaacacct gaacgacaat
     2281 tttctatttc tgttctggat gatgataaca aagaaaatct acctaccgat accgcacctt
     2341 gttttacaat tggtattcct gatgaatat
//
LOCUS       DAPHCV010000287         2350 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.292, whole genome shotgun sequence.
ACCESSION   DAPHCV010000287 DAPHCV010000000
VERSION     DAPHCV010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.292"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(230..>2350)
                     /locus_tag="RH033_004830"
     CDS             complement(230..>2350)
                     /locus_tag="RH033_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HDV2993877.1"
                     /translation="NRGDNRGDIVIAGSQLKAGGNTSLDAANDILLSGAANTQKTTGR
                     NSSSGGGVGVSIGAGGNGAGISVFAGVNAAKGSEKGNGTEWTETTTDSGKTVTINSGR
                     DTVLNGAQVNGNRIIADVGHDLLISSQQDTSKYDSKQTSVAAGGSFTFGSMTGSGYIA
                     ASRDKMKSRFDSVAEQTGMFAGDGGFDITVGRHTQLDGAVIASTATPDKNHLDTGTLG
                     FSDLHNEADYKVSHSGISLSGGGSFGDKFQGNMPGGMISAGGHSGHAEGTTQAAVAEG
                     TITIRDRDNQKQNLANLSRDPAHANDSISPIFDKEKEQRRLQTVGLISDIGSQVADIA
                     RTQGELNALKAAKEATGETLPANATEKQRQEYLAKLRDTQAYRNEMAKYGTGSEIQRG
                     IQAATAALQGLAGGNLAGALAGASAPELAHLLKSTEKDPAVNAIAHAILGGAVAAMQG
                     NNVAAGAAGAATGELAARAITGMLYPGVKQSDLSEEQKQTISTLATVSAGLACGLTGN
                     STASAAVGAQSGKNAVENNSLSDGWNNILPSGTQDYGQAVASWNQYAQDNNLTPEQVQ
                     EGMNRIAIGEGPSWGTTYKVHPVVQAGGDVSFIRGYTLSGTIDDNHISVNQGDIYSIG
                     AHGGASIGLSFGPYFPGLINSNDNDYSINGGFGVGAVGLSTGKDGVSFTFGFGPSWGW
                     SATEIKGVDVNGTSTSEVYRYDFK"
BASE COUNT          538 a          686 c          505 g          621 t
ORIGIN      
        1 tggattacga ttatgatcaa aagccaatcc aaatataaaa acacagataa ccgatagtgc
       61 agaggcattt gctctcatgt cagaactaaa aaatatataa tcaaaaaaca aaaaagagtc
      121 ccgggcaaat tttacaccac cgagatcttt atatataaac aaagcaaata gcactgtgaa
      181 ggaaaatatt gacagcgcaa gcatcaataa aaacagtaaa ctataaaaat tatttaaaat
      241 catatcgata aacctcactt gttgaagtgc cattaacgtc aacgccttta atttcagttg
      301 cactccaacc ccaacttggg ccaaaaccaa aagtgaaact aacaccatct ttacccgtag
      361 aaagccccac tgcaccaacg ccaaaaccac catttatcga ataatcgtta tcatttgaat
      421 ttattaatcc cgggaaatac gggccaaagg ataatccgat cgaggctccg ccatgagcac
      481 ctatactata aatatcccct tgattaactg aaatatgatt gtcatctata gttccgctta
      541 aagtataacc ccggataaag ctaacatcac cgccagcctg aactaccggg tgtactttat
      601 atgttgtccc ccaagaaggc ccttcaccaa tagctatacg attcatccct tcttgaacct
      661 gctccggtgt cagattattg tcctgagcat actgattcca cgaggctacg gcctgtccgt
      721 aatcctgtgt tcctgatggc aggatattgt tccagccatc actgagtgag ttattctcaa
      781 cagcattctt cccgctttgt gctcccactg ctgctgatgc ggtgctgttt ccggtaagac
      841 cgcaggccag ccctgctgac acagttgcca gcgtacttat cgtctgcttc tgctcttcac
      901 tgaggtctga ctgcttcacg ccaggataca gcattccggt aatcgctctg gctgccagct
      961 ctcccgttgc tgcgcctgct gcaccggcag cgacattatt accctgcatt gccgccactg
     1021 cgccgcccag aatggcgtga gcaatggcgt ttactgccgg gtctttttct gttgatttca
     1081 gcagatgtgc cagttccggt gctgaagctc cggccagagc acctgctaaa ttaccccccg
     1141 ccagcccctg aagcgccgct gtcgcggcct ggataccccg ctgaatttca ctgccggtac
     1201 catacttcgc catttcattc ctgtaagcct gcgtgtcacg cagtttcgca agatattcct
     1261 gacgttgttt ctctgttgca ttagcaggaa gtgtttcgcc ggttgcttcc ttcgcggcct
     1321 tcaacgcatt cagttctccc tgtgtacgcg caatatccgc cacctgactg cctatgtcac
     1381 tgataagccc cactgtctgc agacgcctct gctctttctc cttgtcaaat atcgggctga
     1441 tactgtcatt agcgtgcgca gggtcacggc tcaggttcgc cagattctgc ttctgattgt
     1501 ccctgtcccg gatggtgata gtgccttctg ccactgcggc ctgagtcgtt ccttccgcat
     1561 gtccgctgtg acctccggcg gatatcatgc cacccggcat gttaccctga aatttatccc
     1621 cgaagctgcc accaccgctc agactgattc cactgtgact gactttataa tccgcttcgt
     1681 tgtgaaggtc actgaacccc agcgttccgg tatccaggtg gtttttatcc ggtgtggcag
     1741 tggaggcaat caccgcacca tccagttggg tatgtctgcc cactgtaatg tcgaagccac
     1801 catcaccggc aaacattccg gtttgttcag caacggagtc aaagcggctc ttcatcttat
     1861 cccgggaggc agcgatgtaa cctgagccgg tcatggagcc aaaggtaaaa ctgccgccgg
     1921 cagccacgct ggtctgttta ctgtcgtact tactggtgtc ctgctggctg cttatcagca
     1981 ggtcgtggcc cacatcggcg ataatcctgt tgccgttgac ctgagcaccg ttcagtaccg
     2041 tatcccgacc actgttgatg gtgacggttt taccgctgtc tgttgtggtt tcagtccact
     2101 cagtaccgtt acctttctcg ctgccttttg ccgcattaac gccggcaaag acgctgatac
     2161 cggcaccgtt accaccggca ccgatactga cacccacgcc accgccactg ctgctgttcc
     2221 tgcccgttgt tttttgtgtg tttgcggcgc cactcaacag aatatcattc gcggcatcca
     2281 ggcttgtgtt accaccggcc ttaagctggc ttccggcaat cacaatatct ccgcggttat
     2341 cgcccctgtt
//
LOCUS       DAPHCV010000288         2345 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.293, whole genome shotgun sequence.
ACCESSION   DAPHCV010000288 DAPHCV010000000
VERSION     DAPHCV010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.293"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            12..425
                     /gene="hiuH"
                     /locus_tag="RH033_004831"
     CDS             12..425
                     /gene="hiuH"
                     /locus_tag="RH033_004831"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HDV2993878.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            534..1538
                     /gene="msrP"
                     /locus_tag="RH033_004832"
     CDS             534..1538
                     /gene="msrP"
                     /locus_tag="RH033_004832"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HDV2993879.1"
                     /translation="MKKNQFLKESDVTAESVFFMTRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNDLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYAEQVASLYRGLDLRENF
                     "
     gene            1539..2174
                     /gene="msrQ"
                     /locus_tag="RH033_004833"
     CDS             1539..2174
                     /gene="msrQ"
                     /locus_tag="RH033_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HDV2993880.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQVHNKLSV"
BASE COUNT          606 a          562 c          560 g          617 t
ORIGIN      
        1 aggatataaa catgttaaag cgttatttag tactctccgt agtaacggct gcattttcat
       61 taccttcttt ggtctatgct gcacaacaaa acattcttag tgtgcacatt ttgaaccagc
      121 aaacaggaaa acccgctgcc gacgtgacag tcactcttga aaagaaagcg gacaacggct
      181 ggttacaact taataccgcc aaaacagata aggatggacg aattaaggca ctgtggcccg
      241 agcaaactgc aactacgggc gattaccgtg tcgtatttaa aaccggggac tatttcaaga
      301 aacaaaatct tgaaagtttc ttccctgaga tccccgttga atttcatatt aataaagtga
      361 acgagcatta tcatgtgcct ttattactta gccaatatgg gtattcaacc tatcgtggca
      421 gttaatgcat aaatatgtaa aagcagatct ctgctactgt tagggaggac actccctgac
      481 agattaacag taaacggctc ttgctggcta aagacaaaaa agtgtgatgg cttatgaaaa
      541 agaatcaatt tttaaaagaa tcagatgtta cggccgagtc ggtattcttt atgacgcgtc
      601 ggcaggtgtt aaaagcactg ggcatcagcg cggctgcact ttctttgcct cacgctgcgc
      661 atgccgatct gcttagctgg tttaaaggga atgatcgccc acccgccccc gccggaaaac
      721 cgttggagtt cagcaagcct gccgcctggc aaaatgacct gccactgacg ccagcagata
      781 aagtctccgg ttataacaac ttctatgaat tcgggctgga taaagccgat cccgccgcta
      841 atgctggtag cctgaaaacc gatccatgga cactgaaaat cagcggcgaa gtggcaaaac
      901 cattgaccct cgatcacgat gatttaaccc gtcgctttcc gctggaagag cgtatttatc
      961 gtatgcgctg cgtggaagca tggtcgatgg tggtgccgtg gattggtttt ccgctgcaca
     1021 aattgctggc gcttgccgaa cccaccagca atgcgaagta tgtcgctttc gaaacaattt
     1081 atgcaccgga acaaatgcca ggccagcagg accgctttat cggtggcggg ctgaaatatc
     1141 cttatgtcga aggattgcgt ctcgacgaag caatgcatcc gctcacactg atgaccgtgg
     1201 gtgtttatgg caaggcgtta ccgccacaaa atggcgcgcc ggtgcgactg attgtgccgt
     1261 ggaaatatgg ctttaaaggg attaaatcga tagtcagtat taagctgacc cgcgagcgtc
     1321 cgccaaccac ctggaatctg gcagcgcctg acgaatacgg tttttacgcc aacgttaatc
     1381 cgcatgttga tcacccgcgc tggtcacagg ctaccgaacg atttattggt tcaggcggca
     1441 tcctcgatgt acagcgccag ccaacgctac tgtttaatgg ttacgccgag caggtggcat
     1501 cgctgtatcg tggcctggat ttgcgggaga atttctaaat gcgtctgaca gcaaaacagg
     1561 tgacatggct gaaagtttgc ctgcatcttg ccggattgtt gccgtttctc tggctggtct
     1621 gggcgattaa tcacggtgga ttgggtgccg atccggtgaa agatattcag cattttactg
     1681 gtcgcactgc actgaaattt ttgctggcga ccttgttaat cacacctctg gcacgctacg
     1741 caaaacagcc gttattgata cgcactcgcc gcctgttagg cttatggtgc tttgcctggg
     1801 cgacactgca tttaaccagt tacgcattgc tggagctggg cgtgaataat ctggcgttat
     1861 taggtaagga gttaattacc cggccttatt taacgttagg tattatcagc tgggtaattt
     1921 tgcttgcgtt agcgttcact tcaacccagg cgatgcagcg aaaactcggt aaacactggc
     1981 aacagttgca taacttcgtc tatcttgtcg cgatcctggc cccaatacat tatttgtggt
     2041 cagtgaagat tatttcaccg cagccactca tctacgctgg gctggcggta ctgcttttag
     2101 ccttacggta taagaagttg ctttcccttt ttaaccgttt acgcaagcag gttcacaaca
     2161 aattatctgt ataacttcat attgccgaca aagtacgcgt tatccctgcc ggatgcggcg
     2221 cgagcgccct atccggccta cgcagatcac atcaagttat gtttcgtttg tcatcaacct
     2281 caaatcatcg acataattta ttttatttca ataagataga taagcacacc tgagagaact
     2341 attct
//
LOCUS       DAPHCV010000289         2329 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.294, whole genome shotgun sequence.
ACCESSION   DAPHCV010000289 DAPHCV010000000
VERSION     DAPHCV010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.294"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            46..675
                     /locus_tag="RH033_004834"
     CDS             46..675
                     /locus_tag="RH033_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDV2993881.1"
                     /translation="MCEHLITVVEKYGAAERVHLGKQAGNVGRAVTKLPLMGKSLHKT
                     IEKNEVKTAKKLPGPVPALVITAFVARRLLRFRHMLACRRRGLIVLTDRYPQDQIPGA
                     YDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKLNVDLEVACARKPDHKRE
                     SLARKIAITPQLTFGGAQLVDIDANQPLEKVLVDAEKAITDFMTARGYH"
     gene            complement(724..2223)
                     /gene="pitB"
                     /locus_tag="RH033_004835"
     CDS             complement(724..2223)
                     /gene="pitB"
                     /locus_tag="RH033_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HDV2993882.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
BASE COUNT          616 a          608 c          575 g          530 t
ORIGIN      
        1 ccattaattg ccgttattgg tagcgatggt tcaggcaagt caacggtgtg tgaacatctt
       61 attaccgttg tcgaaaaata tggtgctgcc gaaagagttc atttaggaaa acaggccgga
      121 aatgtcggtc gtgcagtgac aaaattaccg ttgatgggaa agtctctgca caagactatt
      181 gaaaaaaatg aagtgaaaac ggcaaaaaaa ttgccgggtc cggttccggc cctggtgatc
      241 accgctttcg tcgcccgccg actgttacgc tttcgtcata tgctcgcctg tcgtcgtcgc
      301 gggctgattg tcttaaccga tcgttatcct caggaccaaa ttcctggcgc ttacgatggt
      361 acggtgttcc cacctaacgt tgaaggcggc cggtttgtct catggctggc aagccaggaa
      421 cgtaaagcat ttcactggat ggcgagccat aagcctgatc tggtcatcaa actcaatgtt
      481 gaccttgaag ttgcctgcgc gcgtaaaccc gaccataaac gggaatcgct ggcgaggaag
      541 attgccataa cgccacagtt aacctttggc ggtgcacaac tggttgatat cgacgccaat
      601 cagccactgg aaaaggtgct ggttgatgca gaaaaagcga ttacggattt tatgaccgcg
      661 cgtggttatc actagtcaaa aatggaaatg cccgatcgcc aggaccgggc attttcagga
      721 aggttaaatc aactgcaatg ctatccagta caacccacca gaaagaaaaa tcgccgccgg
      781 taaagtaaac acccacgcca tcaggatgct ggttaccgtt ttacgctgta acccaccgcc
      841 gtccaccacc atcgtccctg ctaccgcaga cgagaggacg tgtgttgtgg agacgggcat
      901 cccaatataa ctggcaaggc cgatagacac tgccgccgtc atttgtgccg ccatgccttg
      961 cgcatacgtc atgccgcgct taccaatctt ctcaccgatg gtcatcgcta cacgacgcca
     1021 gccaatcatg gtgccaatgc cgagcgccag agctactgcc atgatgatcc acaccggagc
     1081 gtactcaatg gtgcttaaca tatcgctgcg cagttttttc agcaggttct ggtcttcttt
     1141 actgacgcct ggcagtttcg ccagcttcgc ggaggtatca gagatgcaca gcataatgcg
     1201 gcgcagctgg ctgcgctgac tcacgcttaa cggctcgtaa ctttccatat tgcctggcag
     1261 catcgtttta acgcgcgcaa tcgcatcaaa ggtatttgcc ggatgacagt gaaactccgt
     1321 tacttgcgtg ccatcagtcg atgccgcagg caatggaggt tccatcgcaa tcaacttctg
     1381 cggcagttca ggatgctgtt gcaggtagtg ttcgaagttg gtgacggcat cgcgggtacg
     1441 ggtaatttca tagccggacg cattcatatt gacgacgaaa ccagcagggg caatccccac
     1501 cagtaccagc atcaccaggc cgatcccttt ctgcccgtcg ttagcaccgt gcgaaaacgc
     1561 cacgcccgca gcggaaacaa tcagcgcaat acgcgtccag aatggtggct tccgtttgcc
     1621 ttttttcttt ttgcgatctt ccggaatgcg gtgaatacgg tcacgctttt tcgtcccgct
     1681 ccagtagcgg cgcagcagga atatcaggcc tcccgcaatg accaggccga cgatagggga
     1741 aacaatcagc gaggagaaaa ttttggtcac ttcacgcagg tttaacgcat ccatcaccga
     1801 tgagccggtt aacagcgcgt tggttaaacc gatgccgata atcgcaccaa tcaaggtatg
     1861 cgaactggag gccggtaaac caaagaacca cgttcccagg ttccagataa tcgccgccag
     1921 cagcatggaa aagaccatcg ccaggccgtg agttgacccc atattcagca acaaatcggt
     1981 tggtaacata tggacaatgg cataggcaac gctaagtccg cctagcaata cgccaaaaaa
     2041 gttaaaaaat gccgccatca ccacggcaag ctgtggctgc atggctcgag tataaataac
     2101 ggttgccacc gcattcgccg tgtcatgaaa accattgatt gcttcgtaga acaacacaaa
     2161 tgccagagca agtaataaca aaagccccgt gtatatatca aggccaacaa ataaatttag
     2221 catatattag attacgccat tttgaattta cgaacggacg cattatcagt gactttaacg
     2281 gcatgggcaa agtgaaatat ctttttttaa cgataaaaag agtgttatt
//
LOCUS       DAPHCV010000290         2321 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.295, whole genome shotgun sequence.
ACCESSION   DAPHCV010000290 DAPHCV010000000
VERSION     DAPHCV010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.295"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..716
                     /locus_tag="RH033_004836"
     CDS             <1..716
                     /locus_tag="RH033_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDV2993883.1"
                     /translation="AAALGMYIRKGDGQSYETDGNDSKENERELTIQPGIIYDDLKPG
                     EEIGMVKSDRPNPNLETFRNGQLRAVAAGSRLSFSSTARNYNGTYSAQRQELVESTDG
                     YLILQDWFIGAVTRPMYRAWLKQAVASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVK
                     EAEAWKIQIRGGAATESDWVRAGGRNPDDVKRRRKAEIDENRKLDLVFDTDPASDKGG
                     SSAATKRQEPQHTDDQSEE"
     gene            661..>2321
                     /locus_tag="RH033_004837"
     CDS             661..>2321
                     /locus_tag="RH033_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDV2993884.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLHINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGNPVIMPENTFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDEPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQTLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSSYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEG"
BASE COUNT          578 a          546 c          666 g          531 t
ORIGIN      
        1 tcgccgctgc tctggggatg tacatccgga aaggcgacgg acagagctat gaaacggatg
       61 gtaatgacag caaggagaat gaacgcgagc ttaccattca gccaggcatt atttacgacg
      121 atctgaaacc cggcgaagaa atcggaatgg tgaagtcgga tcgtcccaat cctaaccttg
      181 aaacttttcg taatggtcag ttgcgtgccg tggcggcggg cagtcgtctg agtttttcca
      241 gtacggcacg caactataac ggcacttaca gcgcccagcg tcaggaactg gttgagtcta
      301 ctgatggcta cctgatcctg caggactggt ttattggtgc cgtcacccgc ccgatgtatc
      361 gtgcatggct gaaacaggct gtggcatccg gtgttatcag gctaccccgc gatcttgacc
      421 gttcttcact gtataccgcg gtgtattccg gaccagtgat gccgtggatt gaccctgtta
      481 aggaggctga ggcctggaaa atccagattc gtggtggagc ggcgacagaa tcagactggg
      541 tacgtgctgg tggtcgtaat ccggatgatg tcaaacgtcg gcgcaaggcc gaaattgatg
      601 aaaaccgcaa gctggatctg gtatttgata ccgatccggc cagtgataaa ggaggcagca
      661 gtgccgcaac gaaacgacag gagccgcagc acaccgacga ccagtccgaa gaataattcc
      721 tggttcagga tgcaggctgg tcaccagagt gacgcggata tttatattta tgacgagatt
      781 ggtttctggg gtgttacagc gaagcagttt atcagtgatc tgaatgcact gggcgatatc
      841 acccacatta atctccatat taattcaccg ggtggcgatg tctttgaagg catcgccatt
      901 tttaatgcgc tgaaaacaca tggtgcgtcc attaccgttt atgtcgacgg tgtggcggcg
      961 tcaatggcgt cggtcattgc gatggtggga aacccggtca ttatgccgga aaacactttc
     1021 atgatgattc ataaaccatt tggctttacg ggcggtgatg cggaggacat gcgcacctat
     1081 gccgacctgc tcgataaagt tgaggcggtt ctgttacccg cttatgcaca gaaaaccggg
     1141 aaaaccaccg atgaaattgc tgccatgctg gcggatgaga cctggatgtc cggtgccgaa
     1201 tgtctggcac atggatttgc tgatcaggta acgccagccg ttaaggcaat ggcatgtatt
     1261 cagtcaaaac gtacagagga atttaaaaag atgccggaat ccattcgaaa catgattact
     1321 ccgccacgca acagtgctcc acgcgtacag gatgatgaac ctgcagcctc ccggacgcca
     1381 gtgcaggcag cagcacccgt ggtggatgaa aacagtatcc gtgcgcaggt actggcagag
     1441 caaaaagcgc gtgtaaacgg tattaatgat ctgtttgcca tgtttggcgg gcgttatcag
     1501 acgctgcagg ctcagtgtct tgccgatcct gaatgttcgc tggagcaggc ccgcgaaaag
     1561 ctgttgaacg agatggggcg cgagtccacg ccatccaata aaaatacccc ggctcatatt
     1621 tatgccggta acggtaattt tgtgggggac gggatccgcc aggcgctgat ggcgcgtgcc
     1681 ggatttgaaa aaaccgaacg tgataatgtc tacaacggga tgaccctgcg tgaatatgcc
     1741 cgtatgtcac tgactgaacg gggtattggg gtttccagtt ataacccgat gcagatggtc
     1801 ggtgcggcgt tcacacacag tacgtctgac ttcggtaata ttctgctgga tgttgcgaac
     1861 aaagccattc tgcagggctg ggaagatgct cctgaaacct atgaacagtg gacgcggaaa
     1921 ggtcagttgt ctgattttaa aattgcccat cgtgtgggta tggggggctt cagtgctctg
     1981 cgtcaggtgc gtgaaggggc ggaatataaa tacgtcacca ccggagataa acaggccact
     2041 attgcactgg cgacctatgg cgagctgttc agtatcaccc gtcaggccat tatcaatgat
     2101 gatctgaata tgctgaccga tgtcccgatg aagctgggcc gtgcggcgaa atccaccatt
     2161 gccgatctgg tttatgccat tctgacgtct aacccgaaaa tctccacaga taatgtaagt
     2221 ctgttcgata aagcgaaaca tgcaaacgta ctggagagcg ctgcaatgga cgtggcatcg
     2281 ctggataaag cccgccagtt gatgcgcgtt cagaaagagg g
//
LOCUS       DAPHCV010000291         2316 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.296, whole genome shotgun sequence.
ACCESSION   DAPHCV010000291 DAPHCV010000000
VERSION     DAPHCV010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.296"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            81..914
                     /gene="xapA"
                     /locus_tag="RH033_004838"
     CDS             81..914
                     /gene="xapA"
                     /locus_tag="RH033_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HDV2993885.1"
                     /translation="MYQAQFSHNPLYCVDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGYLQGVPVACMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDERFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCELKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            975..2231
                     /gene="xapB"
                     /locus_tag="RH033_004839"
     CDS             975..2231
                     /gene="xapB"
                     /locus_tag="RH033_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HDV2993886.1"
                     /translation="MSIALRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSPTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWHTIWLMFAGYAL
                     FLAVIFFFGFKYNHDPEKITHRSVTH"
BASE COUNT          494 a          501 c          627 g          694 t
ORIGIN      
        1 gaaaaaagtg tattggtatg aggtagggca atctcgttat aaaaaggcgt tagattccac
       61 cctacaaaaa aaggatatat atgtatcagg ctcagttttc tcataaccca ctgtattgcg
      121 tagatattat caagacttat aaacctgatt tcacgccacg agtggccttt attttaggtt
      181 ccgggctggg cgcgctggcc gatcagattg agaacgcggt cgcaatttcc tacgaaaagc
      241 tgcctgggtt cccggtaagt accgtacacg gtcatgcggg tgagctggtg ctgggttatc
      301 tccagggggt gccagtggcg tgcatgaaag gtcgcggaca tttctacgaa ggtcgtggga
      361 tgaccatcat gacggatgca atccgtacct ttaagttgct gggctgcgag ttgctgttct
      421 gcaccaatgc ggctggctca ctgcgtcctg aagtgggggc cggcagtctg gtcgcattga
      481 aagatcacat caacaccatg ccgggaacgc ctatggtggg tcttaatgat gaacgttttg
      541 gtgagcgctt cttctcgctg gcgaatgcct acgatgcgga ataccgcgca ctgttacaaa
      601 aagtggcgaa agaagagggg ttccctctga cggagggcgt gttcgtctcg tatccggggc
      661 cgaatttcga gactgcggcg gaaattcgca tgatgcaaat tattggtggg gatgttgttg
      721 gtatgtctgt ggtgcctgag gttatttcag ctcgccattg cgaacttaaa gtcgttgcgg
      781 tctctgcgat taccaacatg gcggaaggtc tgagtgacgt gaagctttct catgcccaaa
      841 cgctggcagc agcggaactc tcaaagcaaa actttattaa tcttatttgc ggctttctgc
      901 gcaaaattgc ctgagtaaaa caacaataaa cggccttact gagtgaggcc atatgatgcg
      961 acaaggaaac gattatgagc atcgctttgc gcttaaaggt aatgtccttt ttgcaatatt
     1021 ttatctgggg gagctggctg gttaccctcg gttcttacat gattaatacc cttcatttca
     1081 ccggcgctaa tgttggcatg gtttacagtt ccaaagggat cgccgcgatt attatgcctg
     1141 gtataatggg gatcatcgca gacaaatggc tgcgtgcaga acgtgcatac atgctgtgtc
     1201 acctggtgtg tgcgggcgta cttttttatg cggcatccgt aactgatccg gatatgatgt
     1261 tttgggtgat gttagtcaat gcgatggcgt ttatgccgac tattgcgtta tcgaacagcg
     1321 tctcttattc ctgtcttgcc caggcagggc ttgacccggt gaccgctttc ccgcccattc
     1381 gcgtttttgg tacggtgggg ttcattgtcg ccatgtgggc agtaagcctg ctgcatctgg
     1441 aattgagtag tctgcagctg tatatcgcgt ccggtgcgtc attgctgctg tcggcttatg
     1501 cgctgacttt gccgaagatt ccggttgcgg agaaaaaagc gactacatcg cttgccagca
     1561 agctgggtct ggatgccttc gtgctgttta aaaatccacg catggctatc tttttcctct
     1621 tcgccatgat gctgggcgcg gtgctgcaaa ttaccaacgt ttttggcaac ccgttcctgc
     1681 atgatttcgc ccgtaatcct gagtttgctg atagcttcgt ggtgaaatat ccgtccattt
     1741 tactgtcagt ttcacagatg gctgaagtcg gtttcattct gacgataccg ttctttttga
     1801 agcgtttcgg tattaaaacc gtcatgctga tgagtatggt ggcgtggacg ctgcgctttg
     1861 gcttctttgc ctatggcgac ccatcaccaa ccgggtttat tttgctgctg ctgtcgatga
     1921 ttgtttatgg ctgtgcattc gatttcttca atatttctgg ttcggtattt gtcgaacagg
     1981 aagttgattc cagcattcgt gccagcgccc agggcctttt tatgaccatg gtaaatggtg
     2041 tcggcgcatg ggttggctcg attctgagcg gtatggcagt ggattacttc tcggtggatg
     2101 gcgtaaaaga ttggcatacc atttggctga tgtttgcagg gtatgctctt ttcctcgcag
     2161 tgatattttt ctttgggttt aaatataacc atgaccctga aaagataacg catcgttcag
     2221 ttacacatta aaaggattcg cagctctgtt ccttggggct gcttttatga taaaggttaa
     2281 ttagtgaaag atattttatt ttcttctggc gttggt
//
LOCUS       DAPHCV010000292         2311 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.297, whole genome shotgun sequence.
ACCESSION   DAPHCV010000292 DAPHCV010000000
VERSION     DAPHCV010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.297"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1682)
                     /locus_tag="RH033_004840"
     CDS             complement(<1..1682)
                     /locus_tag="RH033_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDV2993887.1"
                     /translation="MSLSFSEAKLEQAIIELLQDQGYQHLIGDNVPRSSLDQVIIEDD
                     LRHYLAARYQPDGITEEEIQRLIKQFTTLPASDLYESNKTFCRWLANGFLFKRDDRQQ
                     KDLYIELLDTRHLPAALCQIFDAEDVPLQQAAELLPSYINPPLNLIKIVNQLKISGKD
                     NQSRIPDGILYINGLPLVVFEFKSAVREQDASIGNAWRQLCKRYRRDIPQLFIYNALC
                     IISDGVNNRMGNLFAPYEYFYSWRKVTGNENREQDGIPSLHSMIQGLFHPVRLLDVIK
                     NFICFPDKARHEVKICCRYPQYYAARKLYYSIKQARKPFGNGKGGTYFGATGCGKSYT
                     MQFLTRLLMKSVEFASPTIVLITDRTDLDDQLSAQMCNAKNYIGDDTILPVTSREDLR
                     NQLAGRNSGGVFLTTIHKFTEDTELLSERSNIICISDEAHRSQVNLDQKVIVDKESGK
                     VRKTYGFAKYLHDSLPNATYVGFTGTPIDATLDVFGEVIDSYTMTEAVQDEITVRIVY
                     EGRAAKVILDSSKLEEVEKYYEECANAGTNEWQIDESKKATATMNAVLGDEDR"
     gene            complement(1777..>2311)
                     /locus_tag="RH033_004841"
     CDS             complement(1777..>2311)
                     /locus_tag="RH033_004841"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDV2993888.1"
                     /translation="VDMKSLPTQGYSISDIIEKPYSGGAKFQNNDTLFARITPCLENG
                     KTGFVDFLDEKETAFGSTEFIVMRGTPQVHYLYVACLARENNFRLHAIQNMVGSSGRQ
                     RVQNSCFDSFYIAIPTPAVMSLFSGKVSSYFDKMYFCNLENKSLTALRDTLLPKLISG
                     ELSLDDLPDLTTDTEAA"
BASE COUNT          588 a          515 c          548 g          660 t
ORIGIN      
        1 cgatcttcat cacccagaac cgcattcatg gttgcggtgg cttttttgct ttcgtcgatt
       61 tgccactcat tggtgcctgc gtttgcgcac tcttcgtaat acttttcgac ttcctccagt
      121 ttgctggagt ccaggatcac tttagccgca cggccttcgt acacgatgcg tacagtgatt
      181 tcatcctgaa cggcttcggt catggtgtag ctgtcgatca cctcaccgaa gacatcgagc
      241 gtcgcgtcaa tcggtgtgcc ggtaaagcca acataggtgg cgtttggcag tgaatcgtgc
      301 aggtatttcg caaagccata cgttttgcgc acttttccgc tttctttatc gacgatgact
      361 ttctggtcga ggttaacctg gctgcgatgt gcttcgtccg agatgcaaat gatattgctg
      421 cgttcagaaa ggagttcggt gtcttcggtg aatttatgga tcgttgtcag gaagacaccg
      481 ccactattgc gtcccgccag ttgattacgc aaatcttcac ggctggtaac gggaaggatg
      541 gtgtcgtcac caatgtaatt tttggcgttg cacatttgcg cagaaagctg atcgtccaga
      601 tcggtgcggt cggtgatcaa aacaatggtc gggctggcaa actctacgct cttcatcaaa
      661 agacgcgtta aaaattgcat ggtgtaactt ttgccacagc ccgttgcgcc aaagtaagtg
      721 ccgcctttac cgttaccgaa aggtttacgc gcttgcttga tgctgtaata gagtttgcgg
      781 gcggcatagt actgcggata tcggcagcaa atttttactt cgtgcctagc tttatccggg
      841 aagcagataa agtttttaat tacatccagc agacgtaccg gatgaaacag cccctgaatc
      901 attgagtgca atgatggaat tccatcctgt tcacggtttt cattaccggt gactttgcgc
      961 catgagtaaa aatattcata gggcgcaaac aggttgccca tccggttatt aactccatcg
     1021 ctaataatgc agagcgcgtt gtagataaac agttgcggaa tatcccggcg atagcgtttg
     1081 cagagttgtc tccaagcatt gccaatacta gcatcctgct cacgcaccgc acttttaaat
     1141 tcaaagacga ccagtggcag accgttgata tagagaatgc cgtcaggaat acgactctga
     1201 ttatctttgc cggagatttt aagctgatta acaatcttaa tcaggttaag tggcgggtta
     1261 atataggagg gcagaagttc cgccgcctgt tgcaatggga catcttcggc atcaaatatc
     1321 tggcacagtg cggcaggtag atgccgggtg tcgagcaatt caatatagag atctttttgt
     1381 tgccgatcgt cgcgtttgaa cagaaaacca tttgccagcc agcgacaaaa tgttttgttg
     1441 ctttcataaa gatcggaagc cggaagcgtg gtgaactgtt tgatcagtcg ctgaatctct
     1501 tcttcagtaa tgccatcagg ctggtagcgt gccgctaaat aatgacggag atcgtcttcg
     1561 ataatgacct gatcgagact cgaacgtggg acattatcgc cgatcaggtg ttgatatccc
     1621 tgatcctgta gcagttcaat gatcgcttgt tctaattttg cttcactgaa agagagactc
     1681 atatcgcgct ccaaccctgg ctaatttctg gtaagcattc ccgtatcagt agaaaacctt
     1741 accagaaagc ccctgatttt acaggggcaa aatgcgttat gcagcttctg tatcggtggt
     1801 gagatccgga agatcatcca gggatagctc accggagatg agtttgggga gcagggtgtc
     1861 gcggagggct gtcagggatt tgttttccaa attacagaaa tacattttat caaagtaact
     1921 actgactttt cctgaaaata agctcattac agcaggtgtt ggtattgcaa tataaaagct
     1981 atcaaagcag ctgttctgta cccgttggcg gccggatgaa ccgaccatgt tttgtattgc
     2041 atgtaacctg aaattatttt ctcgggctaa acaagccaca tacaaataat gtacttgagg
     2101 agtacctcgc atcactataa attcagtaga accaaacgca gtctcttttt catctaaaaa
     2161 atcaacaaat cctgttttac cattttctag gcatggagtt atccgagcga ataaagtatc
     2221 gttattttgg aatttcgctc cccctgaata tggcttttct ataatatctg atattgaata
     2281 tccttgagta ggcaaagatt tcatatcaac a
//
LOCUS       DAPHCV010000293         2300 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.298, whole genome shotgun sequence.
ACCESSION   DAPHCV010000293 DAPHCV010000000
VERSION     DAPHCV010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.298"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..225
                     /gene="trbG"
                     /locus_tag="RH033_004842"
     CDS             <1..225
                     /gene="trbG"
                     /locus_tag="RH033_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbG"
                     /protein_id="HDV2993889.1"
                     /translation="VKKINYPVLYESGITPPLCEVSAPEPDAGGKRIVAYVYKSSRNT
                     VFENPDIVKTCTVRDLKNDFMNCDEKGEGQ"
     gene            222..737
                     /gene="traV"
                     /locus_tag="RH033_004843"
     CDS             222..737
                     /gene="traV"
                     /locus_tag="RH033_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HDV2993890.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            872..1093
                     /gene="traR"
                     /locus_tag="RH033_004844"
     CDS             872..1093
                     /gene="traR"
                     /locus_tag="RH033_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HDV2993891.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQEINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            1086..1559
                     /locus_tag="RH033_004845"
     CDS             1086..1559
                     /locus_tag="RH033_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993892.1"
                     /translation="MHKSEAEKIDSILDEHQQIYRRHNQINNILAYCACIAWIPCIIA
                     AFGVDGIYLKILFAVLACCGAVCFFIFFFTLLPESLMVLSRQSLLQLMRLTEDVPDVR
                     QELLNRLLSGKKLTGRDEQDIRRLWQEKVDAMQESATRQREQDTIRKFTEGNKSE"
     gene            1639..1857
                     /locus_tag="RH033_004846"
     CDS             1639..1857
                     /locus_tag="RH033_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993893.1"
                     /translation="MHTFIFLVLIKNINGGGSWNITPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVKTNEPLNSVMETLNGGGQ"
     gene            1885..2232
                     /locus_tag="RH033_004847"
     CDS             1885..2232
                     /locus_tag="RH033_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993894.1"
                     /translation="MKSIYLKSVLAFIFVGVMAMIVCIPFYIVYLAQQPATPEQLTEI
                     LQETPCAAEAFQETLNYQSEPLTLGKANKIASECRKRNEMAEVKRVSENERNKIREKQ
                     LQALNAAHSAKEH"
BASE COUNT          669 a          469 c          568 g          594 t
ORIGIN      
        1 gtcaaaaaaa taaattatcc ggttctgtat gagtccggaa taacacctcc gttatgtgaa
       61 gtcagtgcac cagaacctga tgcgggtgga aaacgcattg tggcatatgt ttataaatca
      121 tcacggaata cggtttttga aaatcctgac attgtaaaaa cctgtacggt cagggatttg
      181 aagaatgatt ttatgaactg cgatgagaaa ggtgagggac aatgaaacag atttctttat
      241 ttattcctct gctggggact ttattacttt ccggctgtgc cggaacaaat acggaatttg
      301 agtgtaacgc caccacatcc gatacctgta tgacgatgga gcaggccaat gagaaggcca
      361 aaaaactgga gcagtcctca gaagcaaagc cggttgcggc atcactgccg cgcctggctg
      421 aagggaactt ccggacaatg ccggtgcaga ccgtcaccgc gaccactccg tccggcagca
      481 gacctgcggt gacagcacat ccggagcaga aactgctggc accacgcccg ctgtttaccg
      541 ctgcccggga agtgaaaacg gttgttccgg tcagttcagt tacgccggta acacctcctc
      601 gtccgctaag aacgggtgag cagacggcag cattatggat agcgccttat attgataacc
      661 aggatgttta tcatcagcca tccagcgtgt tttttgttat taaaccgtct gcgtggggaa
      721 aaccacgtat taattaactg gccctgaagt tgtatatcag ccggcaatgc cggcgggctt
      781 tctgtattca aaatccgcct gtaaaacgag tgccctgtgc gtgaaaaggg atgggcggta
      841 gcgtgtcaga acatgtcggg aggaatattc cgtgagtgat gaagccgatg aagcatattc
      901 agtgacagaa caactgacca tgacaggaat aaaccggata cgccaggaaa taaatgttca
      961 tggtattcct gtttatctct gtgaagcatg cggaaatcct attccggaag cccggcggaa
     1021 aatatttccc ggtgtgacgt tgtgcgttga atgtcaggcg tatcaggaaa ggcagaggaa
     1081 acactatgca taaatcagaa gctgaaaaaa tagattctat tctggatgaa catcaacaga
     1141 tatatcgcag acataaccaa ataaataaca tcctggcgta ttgtgcgtgt attgcatgga
     1201 ttccctgtat tattgctgcg ttcggcgttg atggtatata tctgaaaatc ctgtttgcgg
     1261 tccttgcctg ctgtggtgcg gtatgctttt ttattttctt cttcaccctc ctgccggaat
     1321 cactcatggt cctcagccgt cagagcctgc ttcagcttat gcgtctgact gaagacgtac
     1381 cagatgtgag acaggaactg cttaaccgtt tgttgtccgg taaaaaactg actggtcggg
     1441 atgaacagga tatccggcgt ctctggcagg agaaagtgga tgcaatgcag gagtctgcca
     1501 cccgtcagcg tgaacaggat actattcgga agtttactga agggaataaa tccgaatgag
     1561 aatctgcagt gttttatgac ctcataagtt aactgaaggg atattcagtg attaatatac
     1621 aagcggagat aataaagaat gcataccttt atttttcttg tgctgataaa aaatattaac
     1681 ggtggcggaa gctggaatat tacacccatg ccaaatacgg acgtatgcgt gcaggttctt
     1741 cggacaatcg aagcacatgg cggcaggggc tttggaaata caggggagtg tattgaagtt
     1801 aaaacaaatg aacctttgaa cagcgtgatg gaaacactaa atggtggtgg gcaatgagtg
     1861 cttactgctt cgaaggaaaa tgaaatgaag tcgatatatt taaaatctgt tctggctttt
     1921 atatttgttg gcgttatggc tatgattgta tgtattcctt tttacatcgt ttaccttgcg
     1981 caacaacctg caacaccaga acaactgaca gagattttac aggaaactcc ctgtgcagca
     2041 gaagcatttc aggagacact taattatcag tctgaacctt taacacttgg taaggcaaat
     2101 aaaatcgcat cagaatgcag aaaaagaaac gaaatggcag aggtgaaacg ggtaagtgaa
     2161 aatgagcgaa ataagatacg tgaaaaacaa cttcaggcat taaatgctgc tcattcagca
     2221 aaggaacatt aatccttgtt tcctgttaaa agcctccatc tcttatttgt gctgtggttt
     2281 caggaatggc actgcctgat
//
LOCUS       DAPHCV010000294         2280 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.299, whole genome shotgun sequence.
ACCESSION   DAPHCV010000294 DAPHCV010000000
VERSION     DAPHCV010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.299"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(22..369)
                     /gene="tnpB"
                     /locus_tag="RH033_004848"
     CDS             complement(22..369)
                     /gene="tnpB"
                     /locus_tag="RH033_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDV2993895.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWSVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(369..1016)
                     /locus_tag="RH033_004849"
     CDS             complement(369..1016)
                     /locus_tag="RH033_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001690050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDV2993896.1"
                     /translation="MNARKAVLADNPELILRVLQLRFDESLSYPRISAQTGISKTAIF
                     SLVRRFHQIFTDWPLSGEYSCVQLARALFPGRYPSAPTVTQTVKTEKNRRNRFSPEFK
                     WRLVQQTLLPGACVAQIARENGINDNLLFNWRHLWRNGGLQPPGEHETSLLPVTLTPE
                     PDNKIPDPVQVEQINTPTDSLCCELVLPAGTLRLKGELTPALLQSLIREMKGSSH"
     gene            complement(1186..>2280)
                     /locus_tag="RH033_004850"
     CDS             complement(1186..>2280)
                     /locus_tag="RH033_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDV2993897.1"
                     /translation="EVSLSEARTKRDEARKLISEGIDPCEQKRAKKVVPDLQLSFEHI
                     ARRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLSTPDLLIPVHAAEAKQIYE
                     IASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALNLSRLPELLSRID
                     GYKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRG
                     SKMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYD
                     TTRDVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLM
                     LQWWADFLDANREECISPFEYAKINNPLRR"
BASE COUNT          544 a          561 c          590 g          585 t
ORIGIN      
        1 atatcctcac tttacaacgg gttatatacg gattccagcg cgttccgttc gtttcgggtg
       61 cttccagtcg atgccctcga gaagcatgga caactgagcc ggagtaaggt gaacctttcc
      121 atcgcgggtg accgaccaga cgaagcggcc ccgctccagg cgtttggtga agaggcacag
      181 tccgtcactg tcagcccaca gcacttttat ctggtcaccc cggcgtccgc ggaagatgaa
      241 cagatgcccg gagaacgggt catccttcag gacgttctga acttttgatg ccaggccgtt
      301 aaaaccattt cgcatgtcgg tgataccggc aaccagccag atacgcgaac cggcagggag
      361 agatatcatc agtggctgct ccctttcatt tcgcggataa gtgactgtaa taacgccggt
      421 gtcagttcac ctttcagcct gagagttccg gccggcagaa ccagctcgca gcacagactg
      481 tcggtcggtg tatttatctg ctcaacctgc acggggtccg ggattttatt atccggctcc
      541 ggcgttaacg tcacgggaag tagcgatgtt tcatgttcgc cgggcggctg caggccgccg
      601 ttacgccaga gatgccgcca gttaaagagc aggttatcgt tgattccatt ctcacgagct
      661 atttgtgcga cacaggcacc gggtaaaaga gtttgctgaa caagtctcca tttaaactcc
      721 ggtgaaaatc ggttccggcg gtttttctct gttttcacag tctgagttac tgtcggggct
      781 gaagggtatc gccccgggaa cagagcccgg gccagttgca cgcaggaata ttccccggaa
      841 agaggccagt cagtgaatat ctggtgaaat cgcctcacca gagaaaaaat ggcggttttg
      901 ctgataccgg tctgcgcaga aatgcgcggg tacgacagtg actcgtcaaa tctcagttgt
      961 agcacacgca ggatcaattc cggattgtct gccagtaccg cttttcgtgc attcatctta
     1021 aatgtccctt tactgtgaaa atggacatta gtatcggaaa caggaaaggg aggcgaaaga
     1081 cggtttaaat gagacggtta cgtattagcc ggaataaaaa agaaatttat tgaagcaggt
     1141 tgctcttccc cctaggggaa gagcatatgt gtgaatggcg ttctgtcacc ttctcagcgg
     1201 attgttaatc tttgcatatt caaacggact gatacactcc tccctgtttg catccagaaa
     1261 atctgcccac cactgtagca tcaagcggcg ttcttccaga tgttctgctt tatgaatgta
     1321 agcagcacgt acaccgttac gttcctgatg gctcatctga cgttcaacag catcgcggga
     1381 ccacaaacct gattctatta acgcactaca tgccatcgcc cggaatccat ggccacagac
     1441 atcccgggtt gtgtcatacc ccataaccct cagggcttta tttacggtgt tttcactgat
     1501 tggtttacgc gggtcatgtg cacccgtaaa tatcagacca ttttcacctg cccatgtctg
     1561 aagttctgtc agtatggcta cagcctgttt actgagtggc acaagatgtg gtgtccgcat
     1621 cttggagccc cggtgtgaga attttacgcc tggcagaggt tcgcgctcag ctggaatagt
     1681 ccacatggca ttgtcaatat caatttcaga ccagcgggca tatctgagtt cactggaacg
     1741 aataaaaacc agtaaattca gcataacggc aagccgggtg acaggctggc ctttataacc
     1801 gtcaatacgc gacagaagtt caggcaggcg tgaaagattc agagcggggc gatgttggcg
     1861 ttttaccgtg gtcaatgcac cagccatatc cagagccggg ttatatctga tgatgccaga
     1921 ctgtacggcg taacgcatta cagcagatat tctttgctgc agacgactgg cgatttcata
     1981 aatttgtttt gcttctgcag catgaacagg gataagcagg tccggggtac tgagtgttgt
     2041 gatatcccgg ttgccgataa aggggaaaac gtgtgtctcg aggcttttga gtactttatc
     2101 gctgtgtgat tgtgcccatt gtttattact ggcatgccag cgtcgtgcaa tatgttcaaa
     2161 agagagctgt aaatcaggga caactttttt agctcttttc tgttcgcaag ggtcaatccc
     2221 ctctgaaatc agttttcggg cctcatcccg ttttgtacga gcttcggaga gagaaacttc
//
LOCUS       DAPHCV010000295         2229 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.300, whole genome shotgun sequence.
ACCESSION   DAPHCV010000295 DAPHCV010000000
VERSION     DAPHCV010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.300"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1106
                     /locus_tag="RH033_004851"
     CDS             <1..1106
                     /locus_tag="RH033_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HDV2993898.1"
                     /translation="IGTKLSASLNGNALTATKLQTPRLVSGVEFDGSKDITLTAAHVA
                     AFARRATDTYADADGGVPWNAESGAYNVTRSGDSYILVNFYTGVGSCRTLQMKAHYRN
                     GGLFYRSSRDGYGFEEDWAEVYTSKNLPPESYPVGAPIPWPSDTVPSGYALMQGQAFD
                     KSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGT
                     KTTSSFDYGTKSTNNTGAHTHSLSGSTGSAGAHTHGNGIRWPGGGGSALAFYDGGGFT
                     YVKDSQYQVSPGTSSSRSYYQRIQTQSAGAHTHSLSGTAASSGAHAHTVGIGAHTHSV
                     AIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            1106..1690
                     /locus_tag="RH033_004852"
     CDS             1106..1690
                     /locus_tag="RH033_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDV2993899.1"
                     /translation="MAFRMSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTVWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     ASLLEAWKKYRVLLNRVDTSTAPDIEWPANPVRE"
     gene            complement(1745..>2229)
                     /locus_tag="RH033_004853"
     CDS             complement(1745..>2229)
                     /locus_tag="RH033_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDV2993900.1"
                     /translation="GTGFYLTHVPESSLISLMDLNETSLNAASTRAGESKIKHKISHD
                     VFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATILGDG
                     VVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKIKGTVVMFSASGKK
                     "
BASE COUNT          581 a          509 c          569 g          570 t
ORIGIN      
        1 ttattggaac caaactgtcc gcaagtctga acggtaatgc attaacagca acaaagctgc
       61 aaacgccaag actggtttct ggtgttgagt ttgatggttc caaagatatt actttaaccg
      121 ccgcgcatgt ggctgctttt gccagaaggg caacggatac atatgccgat gcggatggtg
      181 gcgttccctg gaatgccgaa tcaggcgctt ataatgtcac ccgctctggc gacagctata
      241 ttctggttaa cttctatacc ggagtcggaa gttgccggac attgcagatg aaggcgcatt
      301 acagaaatgg tggtctgttc taccgttctt caagagacgg ttatggtttt gaagaagact
      361 gggcagaagt ttatacctcg aaaaatcttc caccagaaag ctacccagtc ggcgcaccaa
      421 tcccgtggcc atcagatacc gttccgtctg gctatgccct gatgcagggg caggcttttg
      481 acaaatctgc ttacccgaaa ctcgcagccg cttatccgtc aggcgtgatc cctgatatgc
      541 gtggctggac gattaagggc aaacctgcca gtggtcgtgc cgtattgtct caggaacagg
      601 acggcattaa atcgcacacc cacagcgcca gcgcatccag tacggatttg gggacgaaaa
      661 ccacatcgtc gtttgattac ggcactaaat ccacgaataa caccggggcg catacgcaca
      721 gtctgagtgg ctctacgggg tctgccggtg ctcatactca tggtaatggt attcgttggc
      781 caggaggcgg cggttctgcg ttagcatttt atgatggcgg tgggttcact tatgtcaagg
      841 attcacagta tcaagtaagc ccggggactt cttccagtag atcgtattat caacgtattc
      901 agacacagtc agcaggtgct catacccact cgctgtctgg tactgcagca agttctggcg
      961 cacatgcaca tactgtaggt attggtgcgc atacgcactc ggttgcgatt ggctcacatg
     1021 gacacaccat caccgttaac gctgctggta acgcggaaaa caccgtcaaa aacatcgcat
     1081 ttaactatat tgtgaggctc gcataatggc attcagaatg agtgaacaag cacggaccat
     1141 aaaaatttat aacctgctgg ccggaactaa tgaatttatt ggtgaaggtg acgcatatat
     1201 tccgcctcat acaggtctgc ctgcaaacag taccgatatt gcaccgccag atattccggc
     1261 aggcttcgtg gctgttttca acagtgatga ggcatcgtgg catctcgttg aagaccatcg
     1321 tggtaaaacg gtctatgacg tggcttccgg cgacgcgtta tttatttctg aactcggctc
     1381 attaccggaa aatgtcacct ggttatcccc ggaaggggag tttcagaagt ggaacggtac
     1441 agtctgggtg aaggatacgg aagcagaaaa actgttccgg atccgggagg cggaagaaac
     1501 aaaaaacagc ctgatgcagg tagccagtga gcatatagcg ccgcttcagg atgctgtaga
     1561 tctggagatc gcaacggaag aagaggcgtc gttgctggaa gcctggaaga agtatcgggt
     1621 attgctgaac cgtgttgata catcaactgc acctgatatt gagtggcctg caaatcctgt
     1681 cagggagtaa tcattgggat tatgccgcag cacgtcgtgc gcaagaacgt gctgcggctg
     1741 gatgctattt tttccctgaa gcggaaaaca ttactacagt acctttaatc ttggttttaa
     1801 cattctcgaa atgctctgag agtatatgtg ttaagccttc ttcggaatct tttgtgtttg
     1861 aaaagatgcc tttttgattg taaatgcgca tcagtttttg accgaagcta ttgtgcacaa
     1921 cgccgtcacc aagaattgtg gctccgtata gagttccatc gtcagttaag gcctgcgccg
     1981 cattgcgtat tacacagctt tttgtagata tatttccagg caggcagtga agaaggtaaa
     2041 acatggaaat ggaatcaaat tgaccatgta acgccgcggg ataaggttca aaaacatcat
     2101 ggctaatttt atgtttaatt tttgattccc cagcccttgt agatgccgcg ttcaggctag
     2161 tttcgttcaa atccattaaa gatatcagac tactctcagg tacgtgagta aggtaaaacc
     2221 cagttccaa
//
LOCUS       DAPHCV010000296         2178 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.301, whole genome shotgun sequence.
ACCESSION   DAPHCV010000296 DAPHCV010000000
VERSION     DAPHCV010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.301"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>2178
                     /gene="epeA"
                     /locus_tag="RH033_004854"
     CDS             <1..>2178
                     /gene="epeA"
                     /locus_tag="RH033_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter EpeA"
                     /protein_id="HDV2993901.1"
                     /translation="NITFTRIQASDYGAIITNNNDKKSITNLNISTLKDTDINVSVSD
                     VHVLGGKGKPGDLFYDRGTGRYLILKKERYSPFFQDVNNTSVWQYVGKNRNEAIAVVK
                     NEKIKNSSKPYLFHGQLNGNMDINIQSGMGVNTFAIDGSVNIPNGTLSNKLGSLIFQG
                     HPVIHAGVGVSAEQNDWETRYFTLNTLNLDGSDFHLSRNAVMTGNIHATNSSKVTLGS
                     SSVYVDRNDGTGESVNSTSGTASDINEKDLSKFTGDITADNSNIIINNKFEGGVSAVN
                     KSAIDIHSQHAVINRWSDISDNSKLTLKKNATLTVNTGLVNKGTIEIGENAELNLQGY
                     PIADKFIPSLHDLGNVKMTASNATLTAGNYAMFSGEITADEATAVRVNLGSETSTLSE
                     FNPNPELTDLMFDKYNTSWTGKISALKGDASMVNTVWRMTGDSGLNTLKTSKSLTVFS
                     SDNKFSTLTVNDLTTSDSTFVLRSDSTGSDKVVVKNKLEGKNNNLLVDYVANDGKHNS
                     LNLELVSAPTGTAADVFNSQTQNVGFSDVTPVIEQKDSGEKTTWTLKGFNAVANQQSA
                     EKAENFMSAGYKNFLAEVNNLNKRMGDLRDINGEAGAWARIMSGTGSASGGFSDNYTH
                     VQVGVDKKHELDGLDLFTGFTVTHTDSSASADVFSGKTKSVGAGLYASAMFDSGAYID
                     LIGKYVHHDNEYTATFAGLGTRDYSTHSWYAGAEAGYRYHVTED"
BASE COUNT          688 a          446 c          489 g          555 t
ORIGIN      
        1 aatattacat ttacccggat acaggcctcc gattatggtg ccatcattac taacaataat
       61 gataagaaat caataaccaa cctgaatatt agcaccctaa aagacactga tatcaatgtt
      121 tccgttagtg atgttcatgt gcttggcgga aaaggaaaac ctggtgattt attttatgac
      181 agagggacag gcagatacct tattctaaaa aaagaaaggt attcgccatt ttttcaggat
      241 gtaaataata ccagcgtctg gcaatatgtg ggaaaaaacc gtaatgaagc aatagcagta
      301 gttaagaatg aaaaaatcaa aaacagtagc aaaccttatc ttttccatgg gcaattaaat
      361 ggcaacatgg acattaatat tcagagcggc atgggcgtta atacctttgc gatagatggc
      421 tcagtcaata taccaaatgg aactttaagt aataagttag gcagccttat attccaggga
      481 caccctgtca ttcatgccgg ggtcggtgtt tctgcagaac agaatgattg ggagacaagg
      541 tattttaccc tcaacaccct caatctggat ggttctgatt tccatttatc ccgtaatgca
      601 gttatgactg gaaatattca tgccaccaac tcaagtaagg taacgctggg tagttcaagt
      661 gtttatgttg atcgtaatga cggtacagga gagtcagtta actcaacatc tggtacagcg
      721 tcagatataa atgagaagga tttaagcaaa tttactggag atattacagc agacaactca
      781 aacattatca taaacaataa attcgaaggt ggtgtttccg ctgtaaataa aagcgccatt
      841 gatattcata gccaacacgc cgtaattaac agatggagtg atatatcaga caacagtaaa
      901 ctaactttaa agaaaaatgc aacccttaca gttaacactg gattggttaa taaaggcact
      961 attgaaatcg gagaaaatgc ggagttaaat cttcagggat atccgattgc tgataaattc
     1021 attccgtcac tacatgacct ggggaatgtg aaaatgacgg cgtcaaatgc caccctgacg
     1081 gcaggtaatt atgcaatgtt cagcggtgag atcactgccg atgaggccac tgctgtcaga
     1141 gttaacctgg gctcagagac atcaacactc tctgaattta atcctaatcc ggaactgacc
     1201 gacctgatgt ttgataaata taatacatca tggaccggca aaatctctgc cctgaaaggc
     1261 gatgcatcaa tggttaatac cgtatggcgt atgacgggag attccgggct taataccctc
     1321 aaaaccagca aatccctgac ggtattcagc agtgataata aattctccac gctgacagtt
     1381 aatgacctga caaccagtga cagcaccttt gtgctgcgtt ctgactctac aggctctgat
     1441 aaggtggtag taaaaaacaa acttgagggt aaaaacaata atcttcttgt tgattatgtt
     1501 gcaaatgatg gaaaacataa ttcgctaaat cttgaactgg tcagtgcacc gacaggaact
     1561 gctgccgatg tctttaattc acagacacaa aatgtgggtt tcagtgatgt aacgccggtg
     1621 attgaacaga aagatagcgg agagaaaacc acatggaccc tgaagggatt caatgctgta
     1681 gccaatcagc agtcagctga aaaagcggaa aatttcatgt cagcaggcta taaaaatttt
     1741 cttgctgaag tcaacaacct gaacaaacgt atgggtgacc tgcgtgacat caatggcgaa
     1801 gctggcgcat gggcacgcat catgagcggt accggctctg ccagtggtgg tttcagtgac
     1861 aactacacgc acgttcaggt cggggtcgac aaaaaacatg agctggacgg actggatttg
     1921 tttaccggtt tcactgtcac acacactgac agcagtgcct ccgccgatgt tttcagcggt
     1981 aaaacgaagt ctgtgggggc tggcctgtat gcttccgcca tgtttgattc cggtgcctat
     2041 atcgacctga ttggcaagta tgttcaccat gataatgagt acactgcaac ctttgccgga
     2101 ctcggaaccc gtgattacag cacgcattca tggtatgccg gtgcagaagc gggctaccgc
     2161 tatcatgtca ctgaggat
//
LOCUS       DAPHCV010000297         2156 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.302, whole genome shotgun sequence.
ACCESSION   DAPHCV010000297 DAPHCV010000000
VERSION     DAPHCV010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.302"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..232
                     /locus_tag="RH033_004855"
     CDS             <1..232
                     /locus_tag="RH033_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDV2993902.1"
                     /translation="PGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKW
                     QRRTHSLWRHRAWHASAFTGIYNDLAAASTFV"
     gene            130..777
                     /locus_tag="RH033_004856"
     CDS             130..777
                     /locus_tag="RH033_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDV2993903.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGCRIDLRVKTGAEAIRAL
                     STQLPAFRQKLNDGWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLAGAKSGGV
                     FQVVLGAALIAVAWWNPVGWLGAAAVSGMYAAGASMILGGVAQMLAPKARTPTATSTD
                     NGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            838..>2156
                     /locus_tag="RH033_004857"
     CDS             838..>2156
                     /locus_tag="RH033_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HDV2993904.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNIAGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQHCEQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCT"
BASE COUNT          473 a          547 c          696 g          440 t
ORIGIN      
        1 gccgggcgat gtgctgctgt gctgttttgg ttcatcggtg ccgaatcatg ccgccattta
       61 ctgcggcgac ggtgagctgt tgcaccatat tcctgaacaa ctgagcaaac gagagaggta
      121 taccgacaaa tggcagcgac gcacacactc cctctggcgt caccgggcat ggcacgcatc
      181 tgcctttacg gggatttaca acgatttggc tgccgcatcg accttcgtgt aaaaacgggg
      241 gctgaagcca tccgggcgtt gtccacacag ctcccggcgt ttcgtcagaa actgaatgac
      301 ggctggtatc aggtgcgcat tgctgggcgt gatgcaggtg aaaccgaatt atctgcccgt
      361 cttaatgagc cgctggcaaa tggtgccgtg atccacatcg tgccgcgtct ggcgggagct
      421 aaaagtggcg gtgtgtttca ggtggtgctg ggggcggcgc tgattgctgt ggcatggtgg
      481 aaccctgtgg gctggctggg tgccgcggct gtatcgggca tgtatgcggc aggggccagt
      541 atgatcctgg gtggtgtggc ccagatgctg gcaccgaaag cccggacgcc cacagcgacc
      601 agcacggata acggtaagca gaacacctat ttttcatcac tggataacat ggttgcccag
      661 ggcaatgttc tgcctgttct gtacggtgaa atgcgtgtgg ggtctcgtgt ggtttctcag
      721 gagatcagca cggcagatga aggggacggt ggtcaggttg tggtgattgg ccgctgatgc
      781 aaaatgtttt atgtgaaacc gcctccgggc ggttttgtcg tttatggagc atgacgaatg
      841 ggcaaaggca gcagtaaggg gcataccccg cgcgaagcga aggacaacct gaaatccacg
      901 cagttactga gtgtgatcga tgccatcagc gaagggccga ttgaaggtcc ggtggatgga
      961 ttaaaaagcg tgctgctgaa cagtacaccg gtgctggaca gtgaggggaa taccaacatc
     1021 gccggtgtca cggtggtgtt ccgggcaggt gaacaggagc agacaccgcc ggagggattt
     1081 gaatcctccg gatccgagac ggtgctgggt acggaagtga aatacgacac gccgatcacc
     1141 cggaccatca cgtctgcaaa catcgaccgt ctgcgcttta ccttcggtgt gcaggcactg
     1201 gtggaaacca cctcaaaggg ggaccggaat ccatcggaag tccgcctgct ggttcagata
     1261 caacgtaacg gtggctgggt gacggaaaaa gacatcacca ttaagggcaa aaccacctcg
     1321 cagtatctgg cctcggtggt ggtgggtaac ctgccgccgc gcccgtttaa tatccggatg
     1381 cgcaggatga cgccggacag caccacagac cagctgcaga acaaaacgct ctggtcgtca
     1441 tacaccgaaa tcatcgatgt gaaacagtgc tacccgaaca cggcactggt cggcgtgcag
     1501 gtggactcgg agcagttcgg cagccagcag gtgagccgta attatcatct tcgcggacgc
     1561 attctgcagg tgccgtcgaa ctataacccg cagacgcggc aatacagcgg tatctgggac
     1621 ggaacgttta agccagcata cagcaacaac atggcctggt gtctgtggga tatgctgacc
     1681 catccgcgct acggcatggg gaaacgtctt ggtgcggcgg atgtggacaa atgggcgctg
     1741 tatgtcatcg gccagcattg cgagcagtcg gtgccggacg gttttggcgg cacggagccg
     1801 cgcatcacct gtaatgcgta cctgaccaca cagcgtaagg cgtgggatgt tctcagtgat
     1861 ttctgctcgg cgatgcgctg tatgccggta tggaacgggc agacgctgac gttcgtgcag
     1921 gaccgaccgt cggataaggt gtggacctat aaccgcagta atgtggtgat gccggatgat
     1981 ggcgcgccgt tccgctacag cttcagcgcc ctgaaggacc gccataatgc cgttgaggtg
     2041 aactggattg acccgaataa cggctgggag acggcgacag agcttgtgga ggacacgcag
     2101 gccattgccc gttacggtcg taacgtcacg aagatggatg cttttggctg taccag
//
LOCUS       DAPHCV010000298         2112 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.303, whole genome shotgun sequence.
ACCESSION   DAPHCV010000298 DAPHCV010000000
VERSION     DAPHCV010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.303"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(134..1063)
                     /gene="fdhE"
                     /locus_tag="RH033_004858"
     CDS             complement(134..1063)
                     /gene="fdhE"
                     /locus_tag="RH033_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HDV2993905.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTTRIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1060..1695)
                     /gene="fdoI"
                     /locus_tag="RH033_004859"
     CDS             complement(1060..1695)
                     /gene="fdoI"
                     /locus_tag="RH033_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HDV2993906.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(1692..>2112)
                     /gene="fdoH"
                     /locus_tag="RH033_004860"
     CDS             complement(1692..>2112)
                     /gene="fdoH"
                     /locus_tag="RH033_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HDV2993907.1"
                     /translation="LCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKT
                     RGYDNAGLYDPAGVGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAV
                     GFAATFAASIFHYVGVGPNRADEEENNLHEEKDEERK"
BASE COUNT          486 a          613 c          541 g          472 t
ORIGIN      
        1 aataactgta actgttacat catatctgga aaacgcctcg caaaatacga gagattttgc
       61 gggaatagca ggtgtcgtca caccctatga gctgtaatcc gggcgaccag cgccacccgg
      121 aaaacgtcag agattactcc ccttcacccg ggaacaggaa cgggttgatg gaactgcggg
      181 cgtagccttc ttgctccatt cgcgcgtcca gtaccagaga ggcgaggtca tctgccacgg
      241 cttcaacttt cggttctttt tcctgataaa gaatcttcag gtaagtgcca cagtcatcgc
      301 agctttcggc tttaatcgcg gcctgttcgt catccagcga ccagtaatgc agtttgccgc
      361 tctgctcgca gttgctgcat tttacgcgca ccacgtgcca ttcggtttca cacaggttgc
      421 aatgcaggta acgcagaccc tgagtggtgc caatttgcac catgctggac accggcatag
      481 agccacatac cgggcaatat tgacgttgtt cgccgtattc agcgcgggct ttgccgggga
      541 tcagattagc catctgcgcc cagtagagcg acagcgcagc ccagataaac ggcgctttat
      601 cgctgctgac ggacgagaaa tcagaggcaa acagtgcgct ggccatatct tccagctcct
      661 gagtcgatgc cttctccaga ttctcaatca ctgccagcgc cgggccgctc atttcaggtt
      721 tcagctcagc aatcagcgcc atcagcagct tttgccagtg cttatcacgc ggcagaacgt
      781 gaatatccag cgggggcttg ccttgtgcgc tggcttcttt aatgcgcgtg gtcagatcca
      841 tctccagcgg atggtcgtac agcaccactt cctgggcgtg ggcgataagc gcagcaaagc
      901 gcaggtaatc acccagcgga ttattttctg ccagctcgcg cagacgctcg gcgcggcggt
      961 tgtataaatt cttgagccga gggaacaata acggcggaat catatccgcc gtacgtttct
     1021 cgctcgaacc cagctcatct tgcgggatta tgcgaatact cattcagctt tcttttccgt
     1081 tgtcttgcgg acctcacggt accagcgcgg gtgatgtttc ttcgcccagg cgctggtaac
     1141 ccatccttcc accatggcgg taatcgtgcc tttcacccaa agggcggcgt agatatgcac
     1201 catgataacc acaattaacg ccactgcggc aaatgaatgc agcattaacg cgaatcggat
     1261 caccgggatt gagaaagcag gcgcaaaata aggacgccag ataatcacgc cgctcaccag
     1321 cagcagaacc aggaaaataa tcgccgccca gaaaacgcat ttctgaccga agttataacg
     1381 cccggtgtca cctacttcct cgttgacgac gatcttacga atattcttcg cccaaaagat
     1441 atcatcccga ttgattaggt tgtgatgcca gtaacggaaa aacatgatga tgaacgaggc
     1501 aaacataacc acgccgacaa acgggtgcag aatgcgcgcc agctgcggtg tgcccatgat
     1561 ttgcatcaac cagttgaagg acgggaacaa aaagcccagc ccgctcaccg ccgccaggat
     1621 gaagcagaag gcggtgatcc agtggttgat acgttccggc gcggtgtagc gcacgatggt
     1681 gtcacgtcgt ttcatttgcg ctcctcgtct ttctcttcgt gcagattatt ctcttcctca
     1741 tccgcacggt tcggaccgac acccacgtag tggaagatac tggccgcaaa ggtagccgca
     1801 aagccaacag ccgcgagcgg tttccagatg cctttccaga atttcacggt ttcgctgatt
     1861 tccgggttct ccggcaagcc atgatacaga tttggcttgt cagcatggtg cagcacgtac
     1921 ataacatgtg taccaccgac gcctgccggg tcgtacagac ccgcattgtc gtaaccgcga
     1981 gttttcagct ccgccacgcg ctcgctcgcc agcgttttca tcgactcttt cgtaccgaag
     2041 tgaatcgcgc ccgttgggca ggtcttcacg caggccggtt cttgcccaac caccacgcgg
     2101 tcaacgcaca gc
//
LOCUS       DAPHCV010000299         2041 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.304, whole genome shotgun sequence.
ACCESSION   DAPHCV010000299 DAPHCV010000000
VERSION     DAPHCV010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.304"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(83..451)
                     /locus_tag="RH033_004861"
     CDS             complement(83..451)
                     /locus_tag="RH033_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993908.1"
                     /translation="MINNKLIEIDNCLSTPSFFDFLKSLNVDSALDSRDEPEFDDCWM
                     SEFNSLDKESFQDDDIEFIDSLREKAFKYSFRVINNSEISSRISDDIEIISKSFVLGK
                     ENSWSITHLWSSYKNRKFPE"
     gene            complement(451..1095)
                     /locus_tag="RH033_004862"
     CDS             complement(451..1095)
                     /locus_tag="RH033_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993909.1"
                     /translation="MTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRET
                     GLHYNLHRYYDPDVGRFMVTDPIGLRGGLNLYAYAPNPLSWIDPLGLTKKCMGVSESG
                     HHVPAVRKSKGRPFEVSRSDKTRPTLFPRGENPEHNHWRLHHAERDVIGPRQGDFLGS
                     DKELFDAYRKAYSKLDDIRVDVKSPNGTYTLGTNVTPSKAVDLIEVWLKGQGLM"
     gene            complement(1109..>2041)
                     /locus_tag="RH033_004863"
     CDS             complement(1109..>2041)
                     /locus_tag="RH033_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HDV2993910.1"
                     /translation="PVHDTIARVVSCISPAKFHECFINWMRDCHSSDDKDVIAIDGKT
                     LRHSYDKSRRRGAIHVISAFSTMHSLVIGQIRTDEKSNEITAIPELLNMLDIKGKIIT
                     TDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYAISEK
                     SHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYIS
                     SADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINIL
                     TNDKVFKAGLRRKMRKAAMDRNYLASVLAGCGLS"
BASE COUNT          543 a          487 c          364 g          647 t
ORIGIN      
        1 cgggatgttt agtgtgctga acgataatta aataataaaa agccgggaag atttaatttt
       61 ctttccggct ttgtctaatt aattattcag gaaattttct atttttatat gatgaccata
      121 agtgagtgat cgaccaagag ttttcctttc cgagtacaaa acttttggag attatctcaa
      181 tatcatcaga tatacgcgaa gatatttcag aattattaat caccctgaat gagtatttaa
      241 atgccttttc tcgcaaggaa tcaataaact caatatcatc atcctgaaaa ctttccttat
      301 ctaatgaatt aaactcactc atccagcaat catcaaactc cggttcatct cttgagtcta
      361 atgcactatc aacatttaat gatttaagaa aatcaaaaaa tgacggggtg gataaacagt
      421 tatcaatttc aattagctta ttattaatca tcacattaac ccttgtcctt tgagccaaac
      481 ctctattaaa tcaacagcct tggatggtgt cacattagta cctagcgtat aagttccatt
      541 tggagattta acatcaactc tgatgtcatc taattttgaa tacgcttttc tgtaagcatc
      601 aaataactct ttatccgaac caaggaaatc accttgtctt ggacctatca catctcgttc
      661 cgcatgatgt aaacgccagt gattatgttc tggattttct cctctaggga ataatgttgg
      721 gcgagtttta tcagatcggg aaacttcaaa cggacgtcct ttagatttac gaaccgcagg
      781 aacatgatgt ccagattcac tcacgcccat acacttcttc gttaatccaa ggggatcgat
      841 ccagcttaac ggattcggtg cataagcata caggtttaac ccgcctctta gccctatcgg
      901 gtccgttacc atgaaccgcc cgacatccgg atcatagtac ctgtgcagat tgtaatgcag
      961 ccccgtttcc ctgtcgaggt actgtccctg gtagcgcagg ttctgctcca ccccgccgtg
     1021 gtccttctcc tgtatcgtgt taccccagac ctgatacccc gtctcccaca cgattttccc
     1081 gtcactgtcc gtcatctcca gggcaagatt acgaaagccc gcaccccgca aggactgacg
     1141 ccagatagtt tctgtccatg gctgcttttc gcatcttacg tcttaaccct gccttgaata
     1201 ccttatcatt cgtcaaaata ttaatagcga tgtgccgtat ccctgaaaat aattctgctg
     1261 catttcctct tcttattttg cagtcgtctt cattcattac cacgtccaga cgccagtgca
     1321 gcttattctc cacgtgccag tggtttcgga ttgctgtggc gaacttctct gcggttaaat
     1381 cagcagaact gatataatat ctgaccgtca tttctggctc tttcttttgt tctgctatta
     1441 ttgaccgaaa ggagactgcc acgcataatt tcttcagacc tttccattca aacgtgaaat
     1501 caataagttc atcagggaca tcgcaaacaa tatgaagacg gatttcttct ctgccgtgac
     1561 ttttttcact aattgcgtaa ctgtcatgct ctggattatt taattctttc agtgggaatt
     1621 tttcctcaaa ggctttatta agccgcccct gatttccttt tacagcgaat aaataatcac
     1681 cgccctgttt ttgtatcttc tctgcgatat ctttctggca ccccatcgca tcagttgtga
     1741 taatttttcc tttaatatcc aacatgttaa gaagttcagg gatagctgtg atctcattgg
     1801 atttctcatc cgtcctgatc tgtccgatga ccagactgtg cattgttgag aacgcactaa
     1861 tgacatgaat cgctcccctg cggcgactct tgtcataaga atgccggagc gtttttccat
     1921 caattgcaat aacgtcttta tcatctgaag aatggcagtc acgcatccag ttaataaagc
     1981 actcgtgaaa ttttgcagga ctgatacagg atacaactct ggcaatggta tcgtgaacag
     2041 g
//
LOCUS       DAPHCV010000300         2031 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.305, whole genome shotgun sequence.
ACCESSION   DAPHCV010000300 DAPHCV010000000
VERSION     DAPHCV010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.305"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            104..439
                     /locus_tag="RH033_004864"
     CDS             104..439
                     /locus_tag="RH033_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HDV2993911.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            complement(526..1374)
                     /locus_tag="RH033_004865"
     CDS             complement(526..1374)
                     /locus_tag="RH033_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDV2993912.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKRNITRLADRKIWDRLMADTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRS
                     SIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRPDLVEKLNDIIARHYPG
                     ALPPRV"
     gene            1618..1896
                     /locus_tag="RH033_004866"
     CDS             1618..1896
                     /locus_tag="RH033_004866"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993913.1"
                     /translation="MYPRKVRLRRTGRSAAADRQRQLRHQAQWPYTPSGVQGAPHPAR
                     LKKNDGTTMACGGHWHTTPAQVKTEKTARHLQPKKSEISSLFHAYFSR"
     gene            1955..>2031
                     /locus_tag="RH033_004867"
     CDS             1955..>2031
                     /locus_tag="RH033_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDV2993914.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGV"
BASE COUNT          536 a          510 c          504 g          481 t
ORIGIN      
        1 catcatagtc ttatgcagac ggcagaggca gggcgcagcc ctgccaggag tcagtttttg
       61 atgataaaat caggcttcaa catgaaacgg agagacagtg ccaatggctt ctaaaaaatt
      121 ttactcagat gatgacatcc agctggcaaa agctgcctta tcggaattgc cagatctcac
      181 cgctcaacgg aaaacattgc gtgattttct tgatgccatt cgtgatgaca ttattgtcct
      241 ggtaaggaca aagggttata cactggcaga tgttcgtgac acgctcaaaa atgcaggata
      301 tgaagtagga gagaaggcac ttcgtgacat tattcgggaa gcagaatcga aaaaaccatc
      361 ccgccgttct tcctctaaaa cagcacctaa aaagacagat tcaggcagga aagatggcat
      421 cgatatgaac aacaattgat gtgacaatgt cacattgtgt tctgagatta tgatgattac
      481 agaaaaaaaa agccccggca taaatacctg ggcttttctg tttaatcaga ctcgtggcgg
      541 caatgcacca ggataatgcc gtgcaataat atcattcagt ttttcaacaa ggtcaggacg
      601 cttaaagaca atgtgtacgg tccctttctg gtagccacgc accgtaaaat attccccttc
      661 taacagctta ccgacattgt caccgttccc gagaaattca ccatactgcg ccccgatgct
      721 gctccggaag tcaggaacgt tgcgcccctc cagcagataa aaaggacgta ccaggtcatc
      781 cagtgcctcc cgtccgctgc agtccagcgt cacacgcccg ttactccagc ggtacagcag
      841 gttttcaata atgattttct tgcccagacg gcagggatta ttggttctgt aatcccatga
      901 caatttccgg tagacatcaa tcagtccctg ttcaaatgtc tgcatcttgc tggcattcag
      961 atggcgaaaa gttgccagta cattgtccag ggtgatttca ggacaggtgt cgctcatcag
     1021 ctggctgttc cactcatcac gctggcatga actcatgaag gtgtacatcc ccgtatccgc
     1081 catcaggcgg tcccagattt tacggtcggc aagacgggtg atattccttt tcgcttcttc
     1141 cggtttatcc atgcatcgaa cggagtcagt cacacagtct tcaaacccgt agagaaaaat
     1201 gtcaccggaa gcatcgagca gaatcctgcg cgctgtcgcc aggcattcca tcgcctgcat
     1261 gaacagtgca ataccttcgt tacgctgcgc gatgatgcgg tcgatagcta cagacggaat
     1321 aacatctgtc gtgccggcag tattcaggtc agcagtgggt aaagtctgat tcatcacatt
     1381 gtctcctcat cgtcacgact gccgggtcag ccaggtcatt tcccggagga gcgaagctcc
     1441 ttctgccgtc cgggtcttcg gggatttccc cgcagtggac ctttccgacg ggggatgctc
     1501 accacgtcac ggggcagttg tccgggggca gacgcaaaag tttttgacgc ctgcggcaaa
     1561 aagttttgtg gctggtacat ctctcccctg gacgactgaa acgggatgtg gtcgtgtatg
     1621 tacccccgga aagtccgtct gcggagaaca ggccgcagtg cggcggcaga caggcagaga
     1681 cagctgcgcc atcaggcgca gtggccttac acaccgtcag gtgtgcaggg agcgccgcat
     1741 ccggcgcgac tgaagaaaaa cgacggcaca acaatggcat gtggtggtca ctggcacacc
     1801 actcccgcac aggtgaaaac agagaaaacc gcacgacatt tacaaccaaa aaaaagtgaa
     1861 atttcttcac tttttcacgc ttatttttcg cgttgatttg gtgattatga tgataaaata
     1921 aagtgaaatt ttttcacttt ttcaaaggtg ttttatgacc ggctgggaac tgcgaatctg
     1981 gcgaaaaagc atgctctggt cacgcgaaaa agcggccaga gaatttggcg t
//
LOCUS       DAPHCV010000301         2017 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.306, whole genome shotgun sequence.
ACCESSION   DAPHCV010000301 DAPHCV010000000
VERSION     DAPHCV010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.306"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..483
                     /locus_tag="RH033_004868"
     rRNA            <1..483
                     /locus_tag="RH033_004868"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            569..644
                     /locus_tag="RH033_004869"
     tRNA            569..644
                     /locus_tag="RH033_004869"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:603..605,aa:Glu,seq:ttc)
     gene            829..>2017
                     /locus_tag="RH033_004870"
     rRNA            829..>2017
                     /locus_tag="RH033_004870"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          547 a          454 c          589 g          427 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcacctcc
      481 ttaccttaaa gaagcgttct ttgcagtgct cacacagatt gtctgataga aagtgaaaag
      541 caaggcgtct tgcgaagcag actgatacgt ccccttcgtc tagaggccca ggacaccgcc
      601 ctttcacggc ggtaacaggg gttcgaatcc cctaggggac gccacttgct ggtttgtgag
      661 tgaaagtcac ctgccttaat atctcaaaac tcatcttcgg gtgatgtttg agatatttgc
      721 tctttaaaaa tctggatcaa gctgaaaatt gaaacactga acaacgaaag ttgttcgtga
      781 gtctctcaaa ttttcgcaac tctgaagtga aacatcttcg ggttgtgagg ttaagcgact
      841 aagcgtacac ggtggatgcc ctggcagtca gaggcgatga aggacgtgct aatctgcgat
      901 aagcgtcggt aaggtgatat gaaccgttat aaccggcgat ttccgaatgg ggaaacccag
      961 tgtgtttcga cacactatca ttaactgaat ccataggtta atgaggcgaa ccgggggaac
     1021 tgaaacatct aagtaccccg aggaaaagaa atcaaccgag attcccccag tagcggcgag
     1081 cgaacgggga ggagcccaga gcctgaatca gtgtgtgtgt tagtggaagc gtctggaaag
     1141 gcgcgcgata cagggtgaca gccccgtaca caaaaatgca catactgtga gctcgatgag
     1201 tagggcggga cacgtggtat cctgtctgaa tatgggggga ccatcctcca aggctaaata
     1261 ctcctgactg accgatagtg aaccagtacc gtgagggaaa ggcgaaaaga accccggcga
     1321 ggggagtgaa aaagaacctg aaaccgtgta cgtacaagca gtgggagcct cttttatggg
     1381 gtgactgcgt accttttgta taatgggtca gcgacttata ttctgtagca aggttaaccg
     1441 aataggggag ccgaagggaa accgagtctt aactgggcgt taagttgcag ggtatagacc
     1501 cgaaacccgg tgatctagcc atgggcaggt tgaaggttgg gtaacactaa ctggaggacc
     1561 gaaccgacta atgttgaaaa attagcggat gacttgtggc tgggggtgaa aggccaatca
     1621 aaccgggaga tagctggttc tccccgaaag ctatttaggt agcgcctcgt gaattcatct
     1681 ccgggggtag agcactgttt cggcaagggg gtcatcccga cttaccaacc cgatgcaaac
     1741 tgcgaatacc ggagaatgtt atcacgggag acacacggcg ggtgctaacg tccgtcgtga
     1801 agagggaaac aacccagacc gccagctaag gtcccaaagt catggttaag tgggaaacga
     1861 tgtgggaagg cccagacagc caggatgttg gcttagaagc agccatcatt taaagaaagc
     1921 gtaatagctc actggtcgag tcggcctgcg cggaagatgt aacggggcta aaccatgcac
     1981 cgaagctgcg gcagcgacgc ttatgcgttg ttgggta
//
LOCUS       DAPHCV010000302         2000 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.307, whole genome shotgun sequence.
ACCESSION   DAPHCV010000302 DAPHCV010000000
VERSION     DAPHCV010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.307"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..683
                     /locus_tag="RH033_004871"
     CDS             <1..683
                     /locus_tag="RH033_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDV2993915.1"
                     /translation="AAALGMYIRKGDGQSYETDGNDSKENERELTIQPGIIYDDLKPG
                     EEIGMVKSDRPNPNLETFRNAQLRAVAAGSRLSFSSTARNYNGTYSAQRQELVESTDG
                     YLILQDWFIGAVTRPMYRAWLKQAVASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVK
                     EAEAWKIQIRGGAATESDWVRAGGRNPDDVKRRRKAEIDENRKLDLVFDTDPASDKGG
                     SSAATKRQ"
     gene            661..>2000
                     /locus_tag="RH033_004872"
     CDS             661..>2000
                     /locus_tag="RH033_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDV2993916.1"
                     /translation="MPQRNDSSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLHINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGNPVIMPENTFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDEPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQTLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSSYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGA"
BASE COUNT          487 a          473 c          582 g          458 t
ORIGIN      
        1 tcgccgctgc tctggggatg tacatccgga aaggcgacgg acagagctat gaaacggatg
       61 gtaatgacag caaggagaat gaacgcgagc ttaccattca gccaggcatt atttacgacg
      121 atctgaaacc cggcgaagaa atcggaatgg tgaagtcgga tcgcccaaat cctaaccttg
      181 aaacttttcg taatgctcag ttgcgtgccg tggcggcggg cagtcgtctg agtttttcca
      241 gtacagcgcg caactataac ggcacttaca gcgcccagcg tcaggagctg gttgaatcca
      301 ctgatggcta cctgatcctg caggactggt ttattggtgc cgtcacccgc ccgatgtatc
      361 gtgcctggct gaaacaggct gtggcatccg gtgttatcag gctaccccgc gatcttgacc
      421 gttcttcact gtataccgcg gtgtattccg gaccggtgat gccgtggatt gaccctgtta
      481 aggaggctga ggcctggaaa atccagattc gtggtggagc ggcgacagaa tcagactggg
      541 tacgtgcagg tggtcgtaat ccggatgatg tcaaacgtcg gcgcaaggcc gaaattgatg
      601 aaaaccgcaa gctggacctg gtatttgata ccgatccggc cagtgataaa ggaggcagca
      661 gtgccgcaac gaaacgacag tagccgcagt acaccgacga ccagtccgaa gaataattcc
      721 tggttcagga tgcaggctgg tcaccagagt gacgcggata tttatattta tgacgagatt
      781 ggtttctggg gtgttacggc gaagcagttt atcagtgatc tgaatgcact gggcgatatc
      841 acccacatta atctccatat caattcaccg ggtggcgatg tctttgaagg catcgccatt
      901 tttaatgcgc tgaaaacaca tggtgcgtcc attaccgttt atgtcgacgg tgtggcggca
      961 tcaatggcgt cggtcattgc gatggtggga aacccggtca ttatgccgga aaacaccttc
     1021 atgatgattc ataaaccatt tggctttacg ggcggtgatg cggaggacat gcgcacctat
     1081 gccgacctgc tcgataaagt tgaggcggtt ctgttacccg cttatgcaca gaaaaccggg
     1141 aaaaccaccg atgaaattgc tgccatgctg gcggatgaga cctggatgtc cggtgccgaa
     1201 tgtctggcac atggatttgc tgatcaggtg acgccagccg ttaaggcaat ggcatgtatt
     1261 cagtcaaaac gtacagagga atttaaaaag atgccggaat ccattcgaaa catgattact
     1321 ccgccacgca acagtgctcc acgcgtacag gatgatgaac ctgcagcctc ccggacgcca
     1381 gtgcaggcag cagcacccgt ggtggatgaa aacagtatcc gtgcgcaggt actggcagag
     1441 caaaaagcgc gtgtaaacgg tattaatgat ctgtttgcca tgtttggcgg gcgttatcag
     1501 acgctgcagg ctcagtgtct tgccgatcct gaatgttcgc tggagcaggc ccgcgaaaag
     1561 ctgttgaacg agatggggcg cgaatccact ccatccaata aaaatacccc ggctcatatt
     1621 tatgccggta acggtaattt tgtgggggac gggatccgcc aggcgctgat ggcgcgcgcc
     1681 gggtttgaaa aaactgaacg tgataatgtc tacaacggga tgaccctgcg tgaatatgcc
     1741 cgtatgtcac tgactgaacg gggtattggt gtttccagtt ataacccgat gcagatggtc
     1801 ggtgcggcgt tcacacacag tacgtctgac ttcggtaata ttctgctgga tgttgcgaac
     1861 aaagccattc tgcagggctg ggaagatgcc cctgaaacct atgaacagtg gacgcggaaa
     1921 ggtcagttgt ctgattttaa aattgcccat cgtgtgggta tggggggctt cagtgctctg
     1981 cgtcaggtgc gtgaaggggc
//
LOCUS       DAPHCV010000303         1994 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.308, whole genome shotgun sequence.
ACCESSION   DAPHCV010000303 DAPHCV010000000
VERSION     DAPHCV010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.308"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            238..486
                     /gene="repA"
                     /locus_tag="RH033_004873"
     CDS             238..486
                     /gene="repA"
                     /locus_tag="RH033_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HDV2993917.1"
                     /translation="MSQTENAVTSSSGTKRAYRKDNPLTLAERQQASLARKRATHKEL
                     RVFIPAALKAQLQEMCEAEGVTQAEMIAELIKQKSAFS"
     gene            733..807
                     /gene="tap"
                     /locus_tag="RH033_004874"
     CDS             733..807
                     /gene="tap"
                     /locus_tag="RH033_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HDV2993918.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            800..1657
                     /gene="repA"
                     /locus_tag="RH033_004875"
     CDS             800..1657
                     /gene="repA"
                     /locus_tag="RH033_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HDV2993919.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESGAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEVAVASARRSRVEWENRQRKKQGLDALGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDANRERQDIVTLVKRQLTREISEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
BASE COUNT          521 a          511 c          499 g          463 t
ORIGIN      
        1 atttcagggg cagacacccg acatgccaga aacagccggt cccgcccggg gccggcacac
       61 aggttaaggc atttccatct tttcagtcat ttcattatca aaatcacatt aaacggtcgt
      121 aatcagacat gatttgtgcg ccaacacaga tcatcgtcac aactttcaag tcgctgattt
      181 caaaaaactg tagtatcctc tgcgaaacga tccctgtttg agaattgagg aggcgagatg
      241 tcgcagacag aaaatgcagt gacttcctca tcaggtacaa agcgagcata tagaaaagat
      301 aatcctctga cgcttgcaga acgtcagcag gcatccctgg caagaaaacg agccacacat
      361 aaagaactca gagtgtttat tcctgctgcg ttaaaagcgc aattgcagga gatgtgtgaa
      421 gctgagggtg ttactcaggc tgagatgatt gccgaactga taaagcagaa gagtgctttt
      481 agctaactga aatgtagcga catcacattc ttgcctgttt ctggtgtgcg tgatagattg
      541 ctgatcgttt aaggaatttt atggctggcc acgccataag gtggcaagga actggttctg
      601 atgtggatgt acaggagcca gaaaagcaaa aaccccgata atcttcttta actttggcga
      661 gtacagaaag attaccgggg ccatctaaaa accgtatagc cagcaattca gctatgcggg
      721 gagtatagct atatgcccgg aaaagttcaa gacttctttc tgtgctcact ccttctgcgc
      781 attgtaagtg caggatggtg tgactgatct tcaacaaacg tattaccgcc aggtaaagaa
      841 cccgaatccg gtgtttacac cccgtaaagg tgccggaacg ctgaagttct gcgaaaaact
      901 gatggaaaag gcggtgggct tcacctcccg ttttgatttc gccattcatg tggcgcatgc
      961 ccgttcgcgt ggtctgcgtc ggcgcatgcc accggtactg cgtcgccggg ccattgatgc
     1021 gctgctgcag gggctctgtt ttcactatga tccgctggcc aaccgcgtcc agtgctccat
     1081 caccacgctg gccattgagt gcggactggc gacagagtcc ggtgcaggaa aactctccat
     1141 cactcgtgcc acccgtgccc tgacgttcct gtcagagctg gggttgatta cctaccagac
     1201 ggaatatgac ccgcttatcg ggtgctacat tccgaccgac atcacgttca caccggcact
     1261 gtttgccgct ctcgatgtgt ctgaggtggc ggtggcttcc gcacgccgca gtcgtgtgga
     1321 atgggaaaac agacagcgca agaagcaggg actggatgca ctgggtatgg atgaactgat
     1381 agcgaaagcc tggcgttttg tgcgtgagcg tttccgcagt taccagacag agcttaagtc
     1441 ccgtggaata aaacgtgccc gtgcgcgtcg tgatgcgaac agggaacgtc aggatatcgt
     1501 caccctggtg aaacggcagc tgacgcgtga aatctcggaa gggcgcttca ctgccaatcg
     1561 tgaggcggta aaacgcgaag tggaacgtcg tgtgaaggag cgcatgattc tgtcacgtaa
     1621 ccgcaattac agccggctgg ccacagcttc cccctgaaag tgacctcctc tgaataatcc
     1681 ggcccgcacc ggaggcatct gcacgcctga agcctgtcgg cgaacaaaaa aacagcaccg
     1741 catacaaaaa acaacctcat catccacctt caggtgcatc cggtccctcc tgtttttgat
     1801 acaaaacacg cctcacagac gaggaatttt gcttatccac atttagctgc aagggacttc
     1861 cccataaggt tacaaccgtt catgtcataa agcgccatcc gccagcctta caggatgcaa
     1921 tgtatctttt aaacacctgt ttatatctcc tttaaactac ttaactacat tcatttaaaa
     1981 agaaaacctg ttca
//
LOCUS       DAPHCV010000304         1991 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.309, whole genome shotgun sequence.
ACCESSION   DAPHCV010000304 DAPHCV010000000
VERSION     DAPHCV010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.309"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>1991)
                     /locus_tag="RH033_004876"
     CDS             complement(<1..>1991)
                     /locus_tag="RH033_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HDV2993920.1"
                     /translation="DQSVPDGFGGTEPRITCNAYLTTQRKAWDVLSDFCSAMRCMPVW
                     NGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSALKDRHNAVEVNWIDPNNGW
                     ETATELVEDTQAIARYGRNVTKMDAFGCTCRGQAHRAGLWLIKTELLETQTVDFSVGA
                     EGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLDREITLPSSGTTLISLADG
                     SGNPVSVEVQSVTDGMKVKVSRVPDGIAEYSVWGLKLPTLRQRLFRCVSIRENDDGTY
                     AITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHLTAEVTADSGEYQVLARWD
                     TPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFTQLALGNYRLTVRAVNAWGQ
                     QGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTVQFEFWFSEKRIADIRQ
                     VETSARYLGTALYWIAASINIKPGHDYYFYVRSVNTVGKSAFVEAVGQPSDDASGYLD
                     FFKGEIGKTHLAQELWTQIDNGQLAPDLTEIRTSITDVSNEITQTVNKKLEDQSAAIQ
                     QIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTPDGMQSQVLLAADRIAM
                     INPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP"
BASE COUNT          396 a          636 c          502 g          457 t
ORIGIN      
        1 ggatttccac cgctggtaat ggtgggagcc gtcaggcgtt tcaggaacac gtcattcatg
       61 aatatctgat caccctgacc aacaaacatc ggttttgtgt tgccatttgc aggattaatc
      121 atcgcaatcc tgtccgccgc cagcagcacc tgactctgca tgccgtcagg ggtgttctca
      181 ataccggcac cgatacccgc aatataaagg cgtccgtcct gcatctgctg cagcttcact
      241 gcccacatgc tgttcaggtt attatttgta tcaacctgaa ccttctgtat ctgctggatc
      301 gctgcactct ggtcttccag tttcttattg acggtctgtg ttatttcatt gctgacatcc
      361 gttatggacg tcctgatttc agtcaggtca ggcgcaagct gaccgttatc aatctgcgtc
      421 cacagctcct gagccagatg ggttttccct atctcgcctt tgaaaaaatc cagatagcct
      481 gatgcatcat cactcggctg accgacagcc tccacgaatg ccgatttgcc aacggtgttc
      541 acactgcgaa cgtaaaaata ataatcatgg cccggtttga tattgatact ggcggctatc
      601 cagtacagtg ccgtaccaag ataacgcgcg ctggtttcaa cctgcctgat atccgcaatc
      661 cgtttttccg agaaccagaa ctcaaactgt accgtcgggt cataaaccgc aagatgcggc
      721 gtggcggtta tctgaaaata gcccggcgtc agctcaatcc gcgacggcgc tgccggtgcg
      781 gcaatccgga acgataccga cgccggatcg ccctgctgcc cccacgcatt tactgcccgg
      841 actgtcagcc tgtagttccc cagagccagt tgtgtgaagc ggtaagtggt ttccgtcgtc
      901 cgggccgtgc tgaccagccg ctcactgccg tcatccgctg ccacggtcag gcgaagcata
      961 aagctcaccc ccttcaccac cttcggcgtg tcccagcgcg ccagcacctg atattccccg
     1021 ctgtctgcgg tgacttcggc agtcaggtgc tgcaccgctg gcggcgtgac accatttacc
     1081 gtgccgctct ggtcgccgtc aaagtgcgcc ccgttatcca cgatggcctc tttttccggt
     1141 acatgctgca cggcagtgat ggcatacgtg ccgtcatcgt tctcacggat actcacacag
     1201 cgaaacaggc gctgacgcag tgtcggcagc ttcagccccc acacgctgta ttcggctatt
     1261 ccgtcaggaa cccggctcac tttcaccttc atgccgtcgg tgacggactg gacctccacg
     1321 ctgaccggat tgccacttcc gtcagccagg cttatcagcg tggtgccgga ggatggcagc
     1381 gtgatttcac ggtcgagcgt cagcgtccgg gtctggctgt tcaccgccag cacgcgacca
     1441 ccggtgctga taccagcata gtcatcatcg cagatttcaa taacatcgcc cggtacatgg
     1501 cgaagccctt ctgcgcccac actgaaatcc acggtctgcg tctccagcag ctccgtttta
     1561 atcagccaca gcccggcgcg gtgtgcctgc ccccggcagg tacagccaaa ggcatccatc
     1621 ttcgtaacat tacgaccgta acgggcaatg gcctgcgtat cttcaacaag ctctgtcgcc
     1681 gtctcccagc cgttgttcgg gtcaatccag ttcacctcaa cggcattatg gcggtccttc
     1741 agggcgctga agctgtagcg gaacggcgcg ccatcatccg gcatcaccac attactgcgg
     1801 ttataggtcc acaccttatc cgacggtcgg tcctgcacga acgtcagcgt ctgcccgttc
     1861 cataccggca tacagcgcat cgccgagcag aaatcactga gcacatccca cgccttgcgc
     1921 tgtgtggtca ggtacgcatt acaggtgatg cgcggctccg tgccgccaaa gccgtccggc
     1981 actgactggt c
//
LOCUS       DAPHCV010000305         1990 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.310, whole genome shotgun sequence.
ACCESSION   DAPHCV010000305 DAPHCV010000000
VERSION     DAPHCV010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.310"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(144..740)
                     /locus_tag="RH033_004877"
     CDS             complement(144..740)
                     /locus_tag="RH033_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993921.1"
                     /translation="MMKSDEKYQVPAWMRPLLPLLCNTGGNDPEELLNDTETTASANV
                     VRYVLIVAVRSQVDLLQLLYRKGLLRTEIPGGFSPEEAQALLDNLVRSHISKALSGER
                     MAARDRNADLAWIGQQLVDAAWFVRATLEAHGMSVGNESPPAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSCVVREAQDWLRDRKLVDITDILR"
     gene            complement(737..1456)
                     /locus_tag="RH033_004878"
     CDS             complement(737..1456)
                     /locus_tag="RH033_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HDV2993922.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYATAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENVI"
     gene            complement(1453..1887)
                     /gene="psiB"
                     /locus_tag="RH033_004879"
     CDS             complement(1453..1887)
                     /gene="psiB"
                     /locus_tag="RH033_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HDV2993923.1"
                     /translation="MNTELTLNALQSMNAQEYEEIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNGGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
BASE COUNT          435 a          603 c          520 g          432 t
ORIGIN      
        1 cccgcatggc cgggtaaaag cggagccggc ggcgacgtcc ttattccggc gagagcaggg
       61 agcacaccgc atgatctcca gaccacccct ggaacgaaac gatcatgacc ggcatgtccc
      121 tgcgtaaaat atacatgcct ggttcaccgg agaatatctg tgatatccac aagttttctg
      181 tcgcgcagcc agtcctgcgc ttcacgcacc acacaactgt ccggtttcac cgctttcagc
      241 gatgatgcca gtcgcttgat caacatcacc agttcccgtg tctgtatgtc cggcattgtc
      301 tccggcggag cagggggact ctcatttccg acgctcatcc catgcgcttc cagtgtggca
      361 cggacaaacc aggcggcatc gaccagttgc tgtccaatcc aggccagatc ggcatttctg
      421 tcacgggctg ccattcgctc gcccgacagc gccttgctga tatggctgcg caccagatta
      481 tccaggagtg cctgcgcttc ttccggtgaa aagccacctg gtatctctgt gcgcaacagc
      541 cctttcctgt acagaagctg cagcagatca acctgcgacc gcacagcaac tatcagtaca
      601 taacggacaa cattcgcact ggcagtggtt tctgtatcat tcagcagttc ttccggatcg
      661 ttccccccgg tgttgcagag caacggcaac agaggtcgca tccacgcggg aacctggtat
      721 ttttcgtctg atttcatcat attacgttct ccagcttgtt cgggatcatc cactgctcaa
      781 gtgcacgtac ctgtaccggt gcaccacgac cggcctcacc ggcttcaaaa atcagccgcc
      841 agagtggttc ctcctgccag aaaaaccggt caaatgatga cagtgatgga aaacgcccgc
      901 accaccccca gaaaatcgtt tccagatcat gctcctcaac aacaccagac cagcgggaca
      961 accagcttcc gaccgtggcg ggagtatgaa acgcaaggtc aatctgcgcc tgagcaaaac
     1021 atgtctggta ttccagttcc cgtcgttctg cctcacgttc ccgctggcgg ctgtatttct
     1081 gctctgtcag cgtggttttc tgacgacgac gctcaccttc acgaaaaggc acggacggga
     1141 acagacggtg ccccgcatcg gatggcaggg gaagaacaca gacttcccca aaactttcaa
     1201 tcagtttatc cacggcagcc agccacagga gacggttgcc atgaaattcc tccttcgtca
     1261 gcgcaggggc aaaaaagcgg agatcggtca gcgaaatccg gcgactgcca ttcaggcagc
     1321 gaaagaaagc ggtggcataa ggccatgctg aaagtgtgcg tccctgacga cgacgttgtt
     1381 ctgtgacagc caccgcctgc atggcggcct gttgttctgc gctgaggggg atcagtgccc
     1441 gtgaacgtgc gctcatgcct gacctccttc tgccatcagg atatggatga tgtcgttaac
     1501 actgtatccc tgcgcatcca gtgtcgcggc aagcgccagc gtgtgactga tcgcgacagg
     1561 cgtgaagata tgttgcacct gaacgacggc gaaaggctgt ccaccaccat tcaccagaac
     1621 cagcatccag agctgagaga cgtctcccgg cgaacataat gccaggtgga aacgttcgtg
     1681 ggctggcgtg aaacgaacct ggacggggaa aaagcccccg aactcgctgc catactctcc
     1741 gttcatcatc cagttagccg gtgcgtccac ttcacgcatc acctcgtgag tgagattacg
     1801 gcgcatatcg cttcccgcag cacggatttc ttcatattcc tgggcattca tggactgcag
     1861 ggcattcagt gtcagttcgg tattcatcat cgtatttctc cctctgccgc cccgtctccg
     1921 gcggggcggt gtggttgttc atcaggcggc atcagccagg ttatcggtgc gttcagtggt
     1981 atctgtctgt
//
LOCUS       DAPHCV010000306         1952 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.311, whole genome shotgun sequence.
ACCESSION   DAPHCV010000306 DAPHCV010000000
VERSION     DAPHCV010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.311"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(371..913)
                     /locus_tag="RH033_004880"
     CDS             complement(371..913)
                     /locus_tag="RH033_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001502553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HDV2993924.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGRSGN
                     MFNQLLASKKLTKTAINEALRRMKKAGIEKAELEAFLREMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLICILRQRYEGRGMTKRKMAELLNDAHPEWCFSTCEKRIANWL
                     AVAEYALYIPMRESFAEKMA"
     gene            complement(910..1200)
                     /locus_tag="RH033_004881"
     CDS             complement(910..1200)
                     /locus_tag="RH033_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HDV2993925.1"
                     /translation="MVNLRKAAKGQMCQIRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKKGHL"
     gene            complement(1200..1799)
                     /locus_tag="RH033_004882"
     CDS             complement(1200..1799)
                     /locus_tag="RH033_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HDV2993926.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERELLSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDEV
                     EFQQVYKSVLNVLWNWILFRKFSSQEEVENVAAQLLEFA"
BASE COUNT          513 a          515 c          399 g          525 t
ORIGIN      
        1 gtatcagaca acatattgtc ccgctaaaca gcgaattaca aaccaccctg caatgatctc
       61 tcatctcatt ttatatgagt tgacgacatc aggataactc atcatcagcc cctgccaaga
      121 aatatcaaaa ctcccgccag caatgtgtta tcacaatatt gtaaaaaaaa cacagcaccg
      181 aaactataac tggtctctgt tataatttgg agcagaaaga ccagttgccc aactagcagc
      241 attctcccct gctttcctga cgtaaaaaaa ccgcattaag cggttttttt acgatgtcca
      301 tgtctgcaat ccgcctcacg atacagcttt gcgaagcata gcaaaattga agcagtttat
      361 acgtaagaaa tcaagccatt ttctcagcaa atgattcacg catgggaata tatagggcat
      421 actcagcaac agctaaccaa ttagcaatcc gtttttcgca tgtgctaaaa caccactctg
      481 ggtgtgcatc atttagcaat tcagccattt tgcgcttagt catcccccgc ccttcatagc
      541 gttgccggag aatgcaaatc aatcctggat gctctgccag cacctcactt atgactcgat
      601 caatacataa cgcctctgca tcagtacaat gcgccagcca gctcttttgc ttgccgttga
      661 tcatctctcg caaaaacgct tccagctcag ctttctctat tcccgctttt ttcattctgc
      721 gcagggcttc attaatggct gttttcgtca gttttttgga tgccaacaac tgattgaaca
      781 tattccctga cctgccaccg ccaatatacg accagcgccc ccacatacgt agttttccct
      841 gaatccagac actttccagc gtggtgaggc gaaggtgttc tccgcttttt cctgtattcg
      901 ttgggtaaat cataaatgac ctttctttct ccagatttct tgtgtgcgaa aaaccccttc
      961 agcatgcatc aggcgcaatt cttctttggt gtaatcgctg gtttttaccc gcccgtcgat
     1021 tagatcgtgg catgagctac aggctatcgc cgcctgcata tcgtgtggtt ttgtcgctgt
     1081 tccgcacgtc cccgccagcc tgtaatgcgc cagcacagag gtttcgggat tgtgattgca
     1141 gtagccaggg attctgatct ggcacatctg gcctttagcc gctttacgta aattcaccat
     1201 tacgcaaact ccagtagttg tgcggccaca ttttcaactt cctcctgaga ggagaattta
     1261 cggaacagaa tccagttcca cagcacattc agtacagatt tataaacctg ctgaaactcg
     1321 acttcgtcca tattcgcaaa agcgatggat tttgcccgac gcccacggct accgtccgga
     1381 taaatatgct cggtgtaaaa tccggcctga atggttaccc actcgcggaa agcctcaaac
     1441 gactttagca acgccgtatc ccgggttcta cgagtcgcaa cggtgttaag gtattgctct
     1501 gcggcatcac tcagggctgg cgtgtgttcc cgaccaactg attcgcacag gtaatcaacg
     1561 aaaccagaca gcagttctcg ttcgcgaggc gtgatcgccc caccgaccgg agtccagtaa
     1621 tcgaatccca gttgcaggag tttgaaaaaa cgcttgtgga atgcgtagtt acgcacacgc
     1681 ttaaagtctg cgtgtatcca ctcacctatt ttgatttgat gcagaaaatc gcaactctcc
     1741 ggcgtcgccg ggagaagtaa accagaagag gtttgtttga ccagttgtat atgcgccatt
     1801 tctcaatctc tcgatggcgc agtgcagcag atgccagttg ttcaggctga cgtataaagt
     1861 ataaataaac tggttccagt gtaaagcccc cacattgatg gaataaaaat caaacaatag
     1921 attgctggga taaaaataca acgcttatta tt
//
LOCUS       DAPHCV010000307         1942 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.312, whole genome shotgun sequence.
ACCESSION   DAPHCV010000307 DAPHCV010000000
VERSION     DAPHCV010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.312"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            87..464
                     /locus_tag="RH033_004883"
     CDS             87..464
                     /locus_tag="RH033_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDV2993927.1"
                     /translation="MATCTENSSGAACQKAMNERDAVGLALATGTVALLPGSAQVMWG
                     LGASANAGISYLADGTVDPANAAIAGWGNVFSMGNGLAGTVGWNAAGGALGNWIGNKD
                     PLSGAISNGIGSAAGYGIGKGIT"
     gene            477..692
                     /locus_tag="RH033_004884"
     CDS             477..692
                     /locus_tag="RH033_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate cyclase"
                     /protein_id="HDV2993928.1"
                     /translation="MTNAVGKWVTNGWDPKYDPTWIKYAEVNRNDIIREGMKPSNIPG
                     TAGDIGGSIGSELGGKVTDKVSEELKK"
     gene            963..1205
                     /locus_tag="RH033_004885"
     CDS             963..1205
                     /locus_tag="RH033_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993929.1"
                     /translation="MSEKTELEIAKQTLKNSKDPAEREKVQQKYDALLEKDIASDKEV
                     IAACSNGNAGSSACASARLKVIASKEGYEDCPYKRL"
     gene            1267..1942
                     /locus_tag="RH033_004886"
                     /pseudo
     CDS             1267..1942
                     /locus_tag="RH033_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          543 a          359 c          533 g          507 t
ORIGIN      
        1 tttatcgttg atttgttgtt gagaataatt tacttggcgg tagtgaatat gctcatgctg
       61 cgtggatacg ccagcatggt atcgacatgg cgacatgtac agaaaactct tccggtgccg
      121 cctgccagaa agcaatgaat gagagggatg ctgtagggct ggctttggca acaggtactg
      181 tggcactact ccccggtagt gctcaggtaa tgtggggact tggagcaagt gcgaatgctg
      241 gtatcagtta tctggctgat ggtactgtag atccagcaaa cgcagccatt gcaggctggg
      301 ggaatgtgtt cagcatgggt aatggcctgg cggggactgt gggctggaat gctgcaggtg
      361 gtgcgctggg taactggatc ggcaacaaag atcctctgtc tggtgcaatt tcaaatggta
      421 tcggttctgc tgctgggtat ggtatcggta aaggtattac ttaggggaca gataagatta
      481 cgaatgctgt aggaaaatgg gtgacaaatg gctgggatcc taagtacgat ccaacatgga
      541 taaaatatgc tgaagtaaat cgtaacgata tcattcgaga aggcatgaaa ccgagcaata
      601 ttcctggaac tgctggagat attggcggtt ctataggatc cgagctaggt ggaaaagtaa
      661 cagataaagt atctgaggaa ctcaagaaat gaaatattct tttcgagtgg taataggtaa
      721 gtttattata tgcagttcaa ttctcttatt gtgttttatt ggcatgatct tagcggccat
      781 aataactaaa ctaacgtttg tagcgttagc atggtttcaa agcgggattt ttgaagtttt
      841 gtggagcgat attgtttatg ctgctcagtt gggtggtctg ggggggggca tattaggttt
      901 ttgttgggtt ttgttatatc tttttagggc aaagggcttt agttgagaat aactacctga
      961 gcgtgtctga aaagacagaa cttgagatag caaaacagac gcttaaaaac agtaaagatc
     1021 cggcagaacg tgaaaaggta cagcagaaat atgatgcatt gcttgaaaaa gatattgcca
     1081 gtgataagga ggttattgct gcctgcagta atggtaatgc ggggagttca gcgtgtgcta
     1141 gcgcaaggct gaaagttatt gcgtcgaaag aagggtacga agattgccct tataagagac
     1201 tgtaattaaa attgtgtaat tgcctgtttt tgatatgttc actccaacaa cggagacagg
     1261 caaacgatgg acgaaaagaa acttaaagcc cttgcggctg actggccaaa ggccttaaaa
     1321 ccgaagccga cctcaaccag ttttcacgca tgttgacaaa gcttaccgtc gaaacggcgc
     1381 tcaatgcaga actgactgac catctcggtc atgagaaaaa tgctcctaaa tcaggctcta
     1441 atacccgtaa tggctactcg tcaaaaacgg tattgtgcga tgatggcgag atcgaactta
     1501 acacgccgcg tgaccgtgaa aataccttcg aaccgcaact aattaagaag catcacccag
     1561 atggacagcc agattttgtc cctgtatgcc aaaggcatga ctacgcggga aatcgttgcc
     1621 acgttcaaag aaatgtacga cgccgatgtc tcgcccacgc tgatatccaa agtgaccgat
     1681 gcggttaaag agcaggtctc tgaatggcaa aatcgaccgt tggatgcact gtatcccatt
     1741 gtttatcttg actgtattgt ggttaaggtt cgtcacggcg ggagtgtcat taacaaagcg
     1801 gtgttcctcg cgctgggcat caataccgac ggtcagaaag agctgctggg tatgtggctg
     1861 gcagaaaatg aaggtgcaaa gttctggctg ggtgtactga ctgagctgaa aaatcgtggc
     1921 cttcaggaca tcctgattgc ct
//
LOCUS       DAPHCV010000308         1929 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.313, whole genome shotgun sequence.
ACCESSION   DAPHCV010000308 DAPHCV010000000
VERSION     DAPHCV010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.313"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            159..1025
                     /locus_tag="RH033_004887"
     CDS             159..1025
                     /locus_tag="RH033_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDV2993930.1"
                     /translation="MSDITASRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LTEISVLTESIGGKTARDWAMKQDFRCGCWLMDKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYENNSPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLYLFSGKPIPDNR
                     ENITIHLDDHIQSVQGKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIARHYSE
                     ALASTVNKSRKA"
     gene            1097..1360
                     /locus_tag="RH033_004888"
     CDS             1097..1360
                     /locus_tag="RH033_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HDV2993931.1"
                     /translation="MPTSVSLSPYFETFIREQIESGRYNNTSEVIRAGLRALEEREQQ
                     IKLESLQSAVTAGINSGESKSAEEVFGRLTHKYKKMAEGEQPI"
     gene            1357..1683
                     /locus_tag="RH033_004889"
     CDS             1357..1683
                     /locus_tag="RH033_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDV2993932.1"
                     /translation="MKLTVSPLAEHDLEAIGDWIAQDNPVRAISFTEELYQQCLLIAE
                     SPVLYRERPELGAGIRGCSYGRYLLLFRVLDTEVRIERIVHGSRDINRVLAEPDDKPS
                     DHFWQP"
BASE COUNT          586 a          438 c          456 g          449 t
ORIGIN      
        1 tgccctgtat cgaacgggcc cgggaggatg tccgtactct tcgtcatctg cagcttctgc
       61 accagaaccg gtaaatatta ctcaccagac ttcattccac tgacgccagc ctgaacaggg
      121 ctggcgtttt catttatctg caaaaaggaa tatcgattat gtctgatatc acagcctccc
      181 gtccggaagt ggtcaacggg catacggacg ttatctgctc aaccagtatc cgccacattc
      241 tggctgtacg aaagagtaca ctgctgcaaa tcgacacact tatccggcaa ctgactgaaa
      301 tctcagttct gacggaaagc attggaggta aaaccgcccg ggactgggcc atgaaacagg
      361 attttcgctg tggctgctgg ctgatggata aaccggaaac agcaatgaaa gccatcaccc
      421 gcaatctcga tcgcgaaatc tggcgtgacc tgatgcaacg ttccgggatg ctgtcattaa
      481 tggatgcgca ggcccgtgat acatggtacc ggtcactgga atacgataat tttccggaaa
      541 tcagtgaagc gaacattctg agcacatttg aacaactgca ccagaacaaa gatgaagtgt
      601 ttgagcgtgg ggtgatcaat gttttcaggg gactgagctg gaattacgaa aacaattcac
      661 cctgtaaatt tggcagtaaa attatcgtca acaacctggt gaggtgggac cggtgggggt
      721 ttcatcttat caccgggcaa caggcagatc gacttgccga cctggaaaga atgctgtatc
      781 tgttcagcgg caaaccgatc cccgacaacc gggaaaacat caccattcat ctggatgacc
      841 acatccagtc tgttcagggt aaagaggact atgaagatga gatgttcagc atcagatact
      901 ttaagaaggg ctccgcgcac atcacgttca ggaaaccaga actggttgac aggcttaatg
      961 atattattgc cagacactac tcggaagccc tggcaagcac agtgaacaaa agccgcaagg
     1021 cataaaagag gtattttaca attcgcgaat attaactatt attaattgat agtaatcaat
     1081 cggcgagaaa atcaccatgc ctaccagtgt atccctcagt ccttactttg aaacgtttat
     1141 ccgcgaacaa atagaaagtg gtcggtataa caatacaagt gaagtcattc gtgccggact
     1201 tcgtgctctg gaagaacggg aacagcagat aaagcttgaa tcactccaga gcgccgttac
     1261 cgcaggcatc aacagcggtg aaagtaaaag tgctgaagag gtattcgggc gactgacgca
     1321 caaatacaaa aagatggcag aaggtgaaca gccaatatga aactgactgt ttcaccatta
     1381 gcagaacacg atcttgaagc tataggtgac tggatcgctc aggataatcc tgtccgtgca
     1441 atcagtttta ccgaagagct ttaccaacaa tgtctgttga tagccgaatc gccagtactc
     1501 tacagagaaa gacccgagtt aggggcaggg atcagaggtt gttcgtatgg tcgttacctt
     1561 ctgctattca gggtactgga tactgaagtt cgtatcgaac gtatcgttca tggttcccgg
     1621 gatatcaaca gggtacttgc agagccagac gacaaaccat cagatcattt ctggcagcca
     1681 taaacaaaat ctgtgcgtta acgctcccta acacaggtag ccaggtattt tataaatctc
     1741 tgtattttgt tgttacttcg attccgcgat tcagcatatc gaaccagaca aaatgattac
     1801 aacaaagcac aaaaaagagg gattatagcg tcaaaagaat tcgaattgca catgggggcc
     1861 tcattggtgg ccccaaaaag aacaaggata aaacagtcga tacaaaacaa ttggttatat
     1921 aaccagttt
//
LOCUS       DAPHCV010000309         1910 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.314, whole genome shotgun sequence.
ACCESSION   DAPHCV010000309 DAPHCV010000000
VERSION     DAPHCV010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.314"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            176..424
                     /locus_tag="RH033_004890"
     CDS             176..424
                     /locus_tag="RH033_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HDV2993933.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            421..858
                     /gene="umuD"
                     /locus_tag="RH033_004891"
     CDS             421..858
                     /gene="umuD"
                     /locus_tag="RH033_004891"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HDV2993934.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LRLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            858..>1910
                     /gene="umuC"
                     /locus_tag="RH033_004892"
     CDS             858..>1910
                     /gene="umuC"
                     /locus_tag="RH033_004892"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
                     /protein_id="HDV2993935.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPRFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGINHCISPEIFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRILKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADF"
BASE COUNT          501 a          482 c          518 g          409 t
ORIGIN      
        1 aaccgtgcac aaaatgctgt ggataagctg gacacagcag cccacagcag gcatacagcc
       61 acacactcag agaattgagc gttttttagt cacaacaatg acatttgact gtgtgcggat
      121 tcagttatta gaatactgta caaacataca gcataaagaa aagggagatg agaacatgat
      181 ccgcattgaa attcttttcg accgccagag cacaaaaaac ctcaaatcag gcacacttca
      241 ggcgctacag aatgaaatcg aacaacgtct gaaaccacac tacccggaaa tctggctgcg
      301 tatagaccag ggcagcgccc cgtctgtatc tgtcacagga gcccgcaacg acaaggataa
      361 agaacgtatc ctgtctctgc tggaagaaat ctggcaggac gacagctggc tgcctgcagc
      421 atgagtaccg tttatcaccg tccggctgac ccttcaggcg atgacagtta cgtgcgaccg
      481 ttgtttgccg atcgttgcca ggccggtttt ccttcgcctg ccacggatta tgctgagcag
      541 gaactggatc tgaacagcta ttgcatcagc agacctgcag ccaccttctt tctgcgcgcc
      601 agcggtgaat cgatgaacca ggctggcgtg cagaatggtg atctgctggt agtggacagg
      661 gccgaaaaac cacaacacgg ggacatcgtt atcgctgaga tcgacggtga gttcaccgtc
      721 aaacgactgc ggttgcgccc acgcccggca ctggagccgg tttcagacag cccggagttc
      781 cgcacactgt atccggaaaa catctgtatt tttggtgttg tcactcacgt gatacacagg
      841 acgcgggagt tacgctgatg tttgcactgg ctgatatcaa cagtttctac gcctcatgtg
      901 aaaaagtttt ccgcccggac cttcgcaacg aaccggtcat cgtactcagc aataacgatg
      961 gctgtgtgat cgcgcgcagc ccggaggcaa aagcccttgg catcagaatg gggcagccca
     1021 ggtttcaggt gagacaaatg cgcctggaga agaaaataca tgtattttcc agcaattatg
     1081 cgctgtacca cagcatgagc caacgggtta tggctgttct ggagtcgctt tctcccgcag
     1141 ttgagcccta ctcaattgat gaaatgttca ttgatttgcg ggggataaat cattgcatct
     1201 ctccggagat ttttggtcat cagctcaggg aacaggtaaa gagctggaca ggactcacca
     1261 tgggggtggg cattgcgcct acaaaaacgc tggctaaaag tgcccagtgg gcaacaaagc
     1321 aatggccaca gttttccggt gtggtcgcgc tgacggcaga aaaccgtaat cggatcttga
     1381 agctactggg gttgcagcca gttggtgagg tctggggagt aggacgcaga ctgacggaaa
     1441 agctgaatgc gctgggtatt aacacagcac tgcagctggc gcaggctaac acggcattca
     1501 tccggaaaaa cttcagcgtc attcttgagc gtacggtacg cgaactcaac ggcgagtcct
     1561 gcatatctct ggaagaagca ccgccggcaa aacagcagat tgtctgtagt cgcagttttg
     1621 gtgaacgaat cacagacaaa gatgccatgc accaggctgt tgttcagtat gcagagcggg
     1681 ccgcagagaa actacgtggg gagcgtcagt attgccggca ggtgacgaca tttgtacgga
     1741 catccccctt tgcagtaaaa gaaccctgtt acagcaatgc cgctgtggaa aagcttccat
     1801 tgcccacaca ggacagccgg gacattattg ccgccgcatg cagagcctta aaccatgtct
     1861 ggcgtgaagg gtaccgctat atgaaggcag gagtcatgct ggctgatttc
//
LOCUS       DAPHCV010000310         1903 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.315, whole genome shotgun sequence.
ACCESSION   DAPHCV010000310 DAPHCV010000000
VERSION     DAPHCV010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.315"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            67..414
                     /locus_tag="RH033_004893"
     CDS             67..414
                     /locus_tag="RH033_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993936.1"
                     /translation="MPFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTLLMGSLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFWFGLLMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(434..595)
                     /locus_tag="RH033_004894"
     CDS             complement(434..595)
                     /locus_tag="RH033_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993937.1"
                     /translation="MVRLDGSTCLQLWNKEGRVVRREGDPLEVAQWLLACHDAGIEVR
                     VQINESAAP"
     gene            complement(1010..1312)
                     /locus_tag="RH033_004895"
     CDS             complement(1010..1312)
                     /locus_tag="RH033_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993938.1"
                     /translation="MRNRANTSPRDEAAFINGGTAGLDNTKKSSDKSSVVSKAKPVSI
                     SFFEENLKDIDALITAEMVSGKGRVNRSDVVRAAVEALKTLSKTEVSRLIAEVKQR"
     gene            complement(1309..>1903)
                     /locus_tag="RH033_004896"
     CDS             complement(1309..>1903)
                     /locus_tag="RH033_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cobyrinic acid ac-diamide synthase"
                     /protein_id="HDV2993939.1"
                     /translation="VGKSTLAVSIAAYLMSLGNRVIIVDADDQKSVLTWYNNRPEDLP
                     HIPVTGATGNIKAMLKEHEKSYDFVIADCAGRDSAEMRSGLMAADVFISPLRPSQMDL
                     DVVPHTCSVFTAAKDFNEDVRGYLVLNMTPTNMFVNEANEAAEVLKDYPEMHLANSRV
                     CDRKAHRDAWAESMTIFETQNDKAQQEIEALVKEVIL"
BASE COUNT          479 a          451 c          422 g          551 t
ORIGIN      
        1 acagcatgat aataatcaat aacaataagc tgtgtcacgt ttacatcatg gaacggaggt
       61 gctcctgtgc cctttgagca gaaaaaagcc cgggccattg ccctgatgga cagcaaaaag
      121 atgtggcgga gcaattatgc ccctcctctt ctgcgtattc tctggcggct gggtatccgg
      181 cttccgccat taccctttat gccgttctgg caggtcacgc tgttgatggg gagcttatgg
      241 ggcatttcct ggggatgtgc aatgtggttt atctactggg ggccgtcagg aatggtggca
      301 ggtgaggcca ttattataag tatcacgggg ggtttttggt tcggactgct catggcctct
      361 ttccactggt ggcgcaggaa agttaaccgg ctgccaccat gggatgatgt gtaaaccgtg
      421 cacaggcgtg ttcttacgga gcggcacttt catttatctg tacgcgaact tctattcctg
      481 cgtcatgaca ggccagcagc cactgcgcca cttccagcgg atcgccctcc cggcgtacca
      541 ccctgccttc tttattccat aactgcagac aggtgctgcc gtcgagacgc accacaaaat
      601 ccccacggca ggcctgatag gggtccgctt ggaattcgga aaataaacca cgttaagact
      661 attttttagt caaaggactt tcaggaatcg ccagaatgtc gggacaacag cggttttggt
      721 gtccgaaatc gtacaattct gtacatctac gcaagggaat gtacggaagt aagccaaacg
      781 gttatcggac ggccggaaca tttcgtacag cctttacgtt cagtgagtta gctggcctgg
      841 atgtaaccac tcgttcaact caattctctt agcgtgggaa aatttccctt ttccaagcgg
      901 acccctatta atatttgttt tgttttaggt tctattcaac ataggtcatc ctaaaataca
      961 ttgaagataa caagataaca agataacaag ataacaagat aacaaggtgt tatctttgtt
     1021 ttacttctgc tatcaatcgt gatacctctg tttttgatag tgttttaagt gcttctaccg
     1081 ccgccctgac aacatcagaa cgattcactc tgcctttacc tgaaaccatc tcagccgtta
     1141 taagggcatc aatgtctttt aggttttctt caaaaaaaga aatactcact ggttttgctt
     1201 tgcttacaac cgatgattta tctgatgact ttttggtatt atcaaggcca gccgtgccac
     1261 cgttaataaa tgctgcctcg tcacgaggtg aagtgtttgc tctatttctc acagaataac
     1321 ctctttcact aacgcttcga tttcttgctg ggctttatca ttttgcgttt caaaaattgt
     1381 cattgattcg gcccaagcat ctcgatgtgc tttacgatca catacccggg agtttgccag
     1441 atgcatttca gggtaatcct taagtacttc agctgcctca ttggcttcat taacaaacat
     1501 atttgtgggc gtcatgttta aaacgagata tccccgaaca tcctcattaa aatcttttgc
     1561 tgctgtaaaa acggagcatg tatgaggcac aacatccagg tccatctgag atgggcgcaa
     1621 aggagaaatg aatacatctg cggccatcaa tccactacgc atttctgcgc tatcacggcc
     1681 agcacagtct gcaataacaa aatcatagga tttttcgtgt tcctttaaca tggctttgat
     1741 gttacctgtc gcgcctgtaa ccggaatatg agggagatct tcagggcgat tgttgtacca
     1801 ggttaatact gatttctggt catctgcgtc gacaataatc actctattgc ctagtgacat
     1861 cagatacgca gctatagata ccgccagtgt acttttacct acc
//
LOCUS       DAPHCV010000311         1894 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.316, whole genome shotgun sequence.
ACCESSION   DAPHCV010000311 DAPHCV010000000
VERSION     DAPHCV010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.316"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            21..>1894
                     /gene="tssI"
                     /locus_tag="RH033_004897"
     CDS             21..>1894
                     /gene="tssI"
                     /locus_tag="RH033_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDV2993940.1"
                     /translation="MSFAATDYPSGALTGSGTHNVTLSGSAVPDGMLLFASLGGTETV
                     GELFTYSIKLKTPDILNLGYVSPAANLQLKPMVGKDLCVNIELDGGGKRYISGLVTAA
                     RVAGHQGRSVVYELRLEPWLKILTHTSDYKAFQNKNVVEILDEVLDEYPWPVEKRLVE
                     NYPTRAWQVQYGETDFDFVQRLMQEWGIYWWFEHSENSHTLVLADAINVHKACADSPL
                     VCYYQKGLKLDKEFIHTITANESLRSGQWVLNDFDFMKPRSLLKSTVANPRETGLAEY
                     EHYEWPGDYFTKSEGEMLTRIRMEEQRSPGSRVQGSGNIRTLMTGFTFTLENYPTAEV
                     NREYLLVQTTLFIQDNAQHSGQEQHFSYSTSFELHPTSEVYRPQRTLSKPHTKGPQSA
                     IVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDENSSCWVRVSYPWAGKGFGGIQIPRIG
                     QEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGLPGAATQSGFYSHTIGGGPTNANALRF
                     EDKTGGEEIWLHAEKDQRIEVNNNESHWVGNNRLKVIDKTETAIIGEERSLTVQMDDT
                     SLAGQNKTIQAVQNLRLAAGDSIILSCGNTILQMTSDGIFNITCKNFNITATENGKIN
                     T"
BASE COUNT          538 a          425 c          504 g          427 t
ORIGIN      
        1 tctagttaaa aggattaatt atgagttttg cagctacaga ttatccatcc ggcgcactaa
       61 ctggatcggg tacacataac gtgactttga gcggcagcgc agttcctgat ggaatgttac
      121 tgtttgcaag cttagggggt acagaaacag taggggaact atttacttat tctattaaat
      181 taaaaactcc tgacatcctg aacctgggct atgtttcccc cgcagccaac ctgcagctca
      241 aaccgatggt gggcaaagat ctgtgcgtca atatcgaatt ggatggcggc ggaaaacgtt
      301 atatcagcgg gctggtgacg gcggcacgcg tggctggtca tcaaggacgt tcggtggtgt
      361 atgaactgcg gctcgaaccg tggttaaaaa tactgaccca cactagtgac tacaaagcgt
      421 ttcagaataa aaacgtggtg gagatcctgg atgaggtact ggatgagtac ccctggccag
      481 tggaaaaacg actggtggaa aattatccga ctcgcgcctg gcaggtgcag tacggtgaaa
      541 cagatttcga ttttgttcag cgcctgatgc aggagtgggg tatctactgg tggtttgagc
      601 acagcgagaa cagtcacacg ttagtgctgg ctgatgccat taatgtccac aaggcatgcg
      661 cagattcgcc gctggtgtgt tattaccaga aagggctgaa actggacaag gagtttatcc
      721 ataccatcac agcaaatgaa agtctgcgca gcggacagtg ggtgctgaat gatttcgact
      781 ttatgaagcc gcgttcactg ctgaagagca ccgtggctaa tccgcgtgaa accggccttg
      841 ctgaatatga gcactacgaa tggccgggag attacttcac aaagagtgaa ggtgaaatgc
      901 tgacacgcat tcgcatggaa gaacagcgta gcccaggtag tcgggtacag ggcagcggca
      961 atattcgtac cctgatgaca ggttttacct tcaccctgga aaactatccg acagccgaag
     1021 tgaaccggga atatctgctg gtacagacca cgctatttat acaggacaat gcgcaacaca
     1081 gcggccagga gcagcatttc tcttactcaa ccagctttga actgcatccc accagcgaag
     1141 tttaccgccc acaacgaacc ctgagcaaac cccacactaa aggcccgcag agcgccatcg
     1201 tcaccggccc ggcagggcag gaaatctgga cagataaata tggccgggtg aaagttcagt
     1261 ttggctggga tcgttacggt aagaatgacg aaaatagttc ctgctgggtc cgtgtcagtt
     1321 atccgtgggc gggtaaagga ttcggtggca ttcagatccc gcgcattggt caggaagtac
     1381 tggtggattt caaaaacggt gatccggatt taccgataat cgttgggcgt acctacaatc
     1441 aggacaccat gccaccgtgg ggactaccgg gcgcagcgac gcaaagcggg ttttacagcc
     1501 acactatcgg cggcggcccg accaatgcta acgccttgcg ttttgaggat aaaactggcg
     1561 gagaagaaat ctggttgcat gccgagaaag atcaacgcat tgaagtaaat aataatgaaa
     1621 gccactgggt gggcaataat cgccttaaag tcattgataa aacagaaaca gcgataattg
     1681 gtgaggaacg ttctcttaca gttcagatgg atgatacctc ccttgccgga caaaataaaa
     1741 cgattcaggc cgtacagaat ttacgccttg ctgcgggaga ttccataatt cttagttgtg
     1801 ggaacactat cctgcaaatg accagtgacg gaatatttaa tataacctgt aagaatttta
     1861 atattactgc aacagaaaat ggaaagataa atac
//
LOCUS       DAPHCV010000312         1867 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.317, whole genome shotgun sequence.
ACCESSION   DAPHCV010000312 DAPHCV010000000
VERSION     DAPHCV010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.317"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1094
                     /locus_tag="RH033_004898"
     CDS             <1..1094
                     /locus_tag="RH033_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HDV2993941.1"
                     /translation="IGTKLSASLNGNALTATKLQTPRLVSGVEFDGSKDITLTAAHVA
                     AFARRATDTYADADGGVPWNAESGAYNVTRSGDSYILVNFYTGVGSCRTLQMKAHYRN
                     GGLFYRSSRDGYGFEEDWAEVYTSKNLPPESYPVGAPIPWPSDTVPSGYALMQGQAFD
                     KSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGT
                     KTTSSFDYGTKSTNNTGAHTHSISGTANSAGAHQHKSSGAFGGTNTSIFPNGYTAISN
                     PSAGIMSTTSGSGQTRNAGKTSSDGAHTHSLSGTAASAGAHAHTVGIGAHTHSVAIGS
                     HGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            1094..1675
                     /locus_tag="RH033_004899"
     CDS             1094..1675
                     /locus_tag="RH033_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDV2993942.1"
                     /translation="MAFRMSEQPRTITIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLAENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     ISLLEAWKKYRVLLNRVNTTTAPDIEWPVAPIG"
BASE COUNT          522 a          426 c          484 g          435 t
ORIGIN      
        1 ttattggaac caaactgtcc gcaagtctga acggtaatgc attaacagca acaaagctgc
       61 aaacgccaag actggtttct ggtgttgagt ttgatggttc caaagatatt actttaaccg
      121 ccgcgcatgt ggctgctttt gccagaaggg caacggatac atatgccgat gcggatggtg
      181 gcgttccctg gaatgccgaa tcaggcgctt acaatgtcac ccgctctggc gacagctata
      241 ttctggttaa cttctatacc ggagtcggaa gttgccggac cctgcagatg aaggcgcatt
      301 acagaaatgg aggtctgttc taccgttcct caagagatgg ctatggtttt gaggaagact
      361 gggcagaagt ttatacctcg aaaaatcttc caccagaaag ctacccagtc ggcgcaccaa
      421 tcccgtggcc atcagatacc gttccgtctg gctatgccct gatgcagggg caggcttttg
      481 acaaatctgc ctacccgaaa cttgcagcgg cttatccgtc aggcgtgatc cctgatatgc
      541 gtggctggac gattaagggc aaacctgcca gtggtcgggc cgtattgtct caggaacagg
      601 acggcattaa atcgcatacc cacagcgcca gcgcatccag tacggatttg gggacgaaaa
      661 ccacatcgtc gtttgattac ggaaccaaat ccacgaataa caccggggcg catacccata
      721 gtattagcgg gaccgcaaat agtgccggtg cgcaccaaca caagagttcc ggtgcatttg
      781 gtggcacgaa cacgagcatt ttccctaatg gttataccgc gatttcaaat ccaagcgcgg
      841 ggattatgag cacaacaagc ggtagtggcc agactcgtaa tgcagggaag acatcatcag
      901 atggtgctca tacccactca ctgtccggca ctgctgcaag cgcaggtgca cacgcacata
      961 ctgtcggtat tggtgctcat acgcactcgg ttgcgattgg ctcacatgga cacaccatca
     1021 ccgttaacgc tgctggtaac gcggaaaaca ccgtcaaaaa catcgcattt aactatattg
     1081 tgaggcttgc ataatggcat tcagaatgag tgaacaacca cggaccataa caatttataa
     1141 tctgctggcc ggaactaatg aatttattgg tgaaggtgac gcatatattc cgcctcatac
     1201 aggtctgcca gcaaacagta ccgatattgc accgccagat attccggctg gctttgtggc
     1261 tgttttcaac agtgatgagg catcgtggca tctcgttgaa gaccatcggg gtaaaacggt
     1321 ctatgacgtg gcttccggcg acgcgttatt tatttctgaa ctcggtccgt tagcggaaaa
     1381 tgttacctgg ttatcgccgg aaggggagtt tcagaagtgg aacggcacag cctgggtgaa
     1441 ggatacggaa gcagaaaaac tgttccggat ccgggaggca gaagaaacaa aaaacagcct
     1501 gatgcaggta gccagtgagc atattgcgcc acttcaggat gctgtagatc tggaaatcgc
     1561 aacggaggaa gaaatctcgt tgctggaagc ctggaagaag tatcgggtgt tgctgaaccg
     1621 tgttaataca acaactgcac cggatattga atggccagta gcacctatag ggtaaattcg
     1681 taatgattac ctaaatacgt tattcttttg ttaaaaaagt gattttgcta taggtaggga
     1741 gtatgttctt ggattaagaa taaaaaatcg ccatgaaaga gcttcatggc gagtacataa
     1801 acgtacaaaa tgtgatgaaa gtagagtggc gtaagccact atattaagat atgacaatag
     1861 cgaagaa
//
LOCUS       DAPHCV010000313         1849 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.318, whole genome shotgun sequence.
ACCESSION   DAPHCV010000313 DAPHCV010000000
VERSION     DAPHCV010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.318"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..276)
                     /gene="tnpB"
                     /locus_tag="RH033_004900"
     CDS             complement(<1..276)
                     /gene="tnpB"
                     /locus_tag="RH033_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDV2993943.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWSVTRDGKVHLTPAQ"
     gene            complement(276..926)
                     /locus_tag="RH033_004901"
     CDS             complement(276..926)
                     /locus_tag="RH033_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001690050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDV2993944.1"
                     /translation="MNARKAVLADNPELILRVLQLRFDESLSYPRISAQTGVSKTAIF
                     SLVRRFHQIFADWPLSGEYSCMQLARALFPGRYPSAPTVTQPVKTEKIRRNRFSPEFK
                     WRLVQQTLLPGACVAQIARENGINDNLLFNWRHLWRNGGLQPPGEHETSLLPVTLTPE
                     PDNKIPAPVQIPEQINTLPDSLCCELVLPAGTLRLKGELTPALLQTLIREMKGSSH"
     gene            1013..1290
                     /locus_tag="RH033_004902"
                     /pseudo
     CDS             1013..1290
                     /locus_tag="RH033_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1461..>1849)
                     /locus_tag="RH033_004903"
     CDS             complement(1461..>1849)
                     /locus_tag="RH033_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV2993945.1"
                     /translation="LVYGSVSGKQFAWKDQGMHLSMKDKDYNPLIGRTGVDVGKSFSG
                     KDWKVTARAGLGYQFDLLVNGETVLRDASGEKRIKGEKDSRMLMSVGLNAEIRDNVRF
                     GLEFEKSAFGKYNVDNAVNANFRYSF"
BASE COUNT          453 a          476 c          488 g          432 t
ORIGIN      
        1 ctgagccgga gtaaggtgaa cctttccatc gcgggtgacc gaccagacga agcggccccg
       61 ctccaggcgt ttggtgaaga ggcacagtcc gtcactgtca gcccacagca cttttatctg
      121 gtcaccccgg cgtccgcgga agatgaacag atgcccggag aacgggtcat ccttcaggac
      181 gttctgaact tttgaggcca ggccgttaaa gccatttcgc atatcggtga taccggcaac
      241 cagccagata cgcgaaccgg cagggagaga tatcatcagt ggctgctccc tttcatttcg
      301 cggataagtg tctgtaataa cgccggtgtc agttcacctt tcagcctgag agttccggcc
      361 ggcagaacca gctcgcagca cagactgtct ggcagtgtat ttatctgttc aggtatctgc
      421 actggtgccg ggattttatt atccggctcc ggcgttaacg tcacgggaag tagcgatgtt
      481 tcatgttcgc cgggcggctg caggccgccg ttacgccaga gatgccgcca gttaaagagc
      541 aggttatcgt tgattccgtt ctcgcgagct atttgtgcga cacaggcacc gggtaaaaga
      601 gtttgctgaa caagcctcca tttaaactcc ggtgaaaatc ggttccggcg gattttctct
      661 gttttcacag gctgagtcac tgtcggggct gaagggtatc gcccagggaa aagagcccgg
      721 gccagttgca tgcaggaata ttcaccggaa agaggccagt cagcgaatat ctggtgaaat
      781 cgcctcacca gagaaaaaat ggcggttttg ctgactccgg tctgcgcaga aatgcgcggg
      841 tacgacagtg actcgtcaaa tctcagctgt agcacacgca ggatcaattc cggattatct
      901 gccagtaccg cctttcgtgc attcatctta aatgtccttt tactgcgaaa atggacatta
      961 gtatcggaaa caggaaaggg aggcgaaaga cggtttaaat gatgcgctta cgcatgaatg
     1021 gatggtggag aagaatggta ctctgtcgaa aaaatccaga ctgggcgaag cgttcagcta
     1081 tgtactgaat cagtgggatg ccctctgtta ttacagtgat gacggtctgg cggaggcgga
     1141 taataatgct gcggaaagag cgcttcgtgc agtctgaacg gtatcgatcc ggaagcgtat
     1201 ctgcgccata tcctgagcgt actgccggaa tggccttcca accgagttga tgaactcctg
     1261 ccatggaacg tagtactcac caataaataa gcgtcaatac ggtgctccgt tgacgcttac
     1321 aaaaagccgg cattactgcc agctttagtc atcgcagtta agtaaaccag tcgactgaca
     1381 tcccggaaat gtctctcaga catatcaggg cggcaggcac tgagcgtaaa gggccctcag
     1441 gcccttttga atacggagta tcagaacgag taacggaagt tagcgttgac tgcattatca
     1501 acgttgtact taccaaaggc ggatttctca aactccagtc caaagcggac gttatccctg
     1561 atttctgcat tcaggccaac ggacatcagc atacggctgt ccttttcacc tttgatgcgt
     1621 ttttcaccag atgcatcccg caatacggtt tcgccgttaa ccagcaggtc gaactggtag
     1681 cccagaccgg cacgggctgt cactttccag tccttaccag agaaggattt acccacatcc
     1741 acaccagttc ggccaatcag cggattgtag tccttgtcct tcatggacag atgcattccc
     1801 tggtccttcc atgcaaactg tttaccggat acagaaccgt aaaccagct
//
LOCUS       DAPHCV010000314         1832 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.319, whole genome shotgun sequence.
ACCESSION   DAPHCV010000314 DAPHCV010000000
VERSION     DAPHCV010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.319"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            120..335
                     /gene="essD"
                     /locus_tag="RH033_004904"
     CDS             120..335
                     /gene="essD"
                     /locus_tag="RH033_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HDV2993946.1"
                     /translation="MKSMDKLTTGVAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            340..651
                     /locus_tag="RH033_004905"
     CDS             340..651
                     /locus_tag="RH033_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HDV2993947.1"
                     /translation="MTQDYELVVKGVRNFENKVTVTVALQDKERFDGEIFDLDVAMDR
                     VEGAALEFYEAAARRSVRQVFLEVAEKLSEKVESYLQHQYSFKIENPANKHERPHHKY
                     L"
     gene            648..1181
                     /locus_tag="RH033_004906"
     CDS             648..1181
                     /locus_tag="RH033_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDV2993948.1"
                     /translation="MNTKIRYGLSAAVLALIGAGASAPQILDQFLDEKEGNHTMAYRD
                     GSGIWTICRGATVVDGKTVFPNMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            1178..1675
                     /locus_tag="RH033_004907"
     CDS             1178..1675
                     /locus_tag="RH033_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HDV2993949.1"
                     /translation="MNQIFMVIFLVLSGFIVGNVWSDRGWQKKWAERDAAALSQEVNA
                     QFAARIIEQGRTIARDEAVKDAQQKSAEISARAAYLSDSVNQLRAEAKKYAIRLDAAK
                     HTADLAAAVRGKTTKTAEGMLTNMLGDIAAEAQLYAEIADERYIAGVTCQQIYESLRD
                     KKHQM"
BASE COUNT          535 a          369 c          462 g          466 t
ORIGIN      
        1 catttttaat gtgtatgttt ctttagcgcc gttgagaaca acgtgtgctg tcaaaactac
       61 cccgtagact ccgatctttt caaacatatt gcaccatccg tgtacatcgg ggtgaggata
      121 tgaaatcaat ggataagtta acaacaggtg ttgcctatgg cacatcggcg ggtaatgctg
      181 gtttctgggc attgcagtta ctcgataaag taactccgtc acagtgggct gcaatcggtg
      241 tgctgggtag cctggttttt ggcctgctga cgtatctgac aaatctttat ttcaagatta
      301 aagaagacag gcgtaaggct gcgagaggag agtaatccaa tgactcaaga ctatgaactg
      361 gttgtgaaag gagtccgtaa ttttgagaat aaagttacgg taactgtagc cttacaggac
      421 aaagaacgct ttgacggtga aatttttgac ctggatgtcg ccatggaccg tgttgaagga
      481 gctgcgctgg agttttatga ggcagcagcc agaaggagcg tccggcaagt cttcctggaa
      541 gtagcagaaa aattgtcaga aaaagttgag tcttatctgc agcatcagta ctcctttaag
      601 attgaaaatc ctgccaataa gcacgagcgt cctcatcata aatatctatg aacacaaaaa
      661 tcagatacgg cctgtcggct gccgttctgg cgctgattgg tgctggcgca tctgctcctc
      721 agatacttga ccagtttctg gacgaaaaag aaggtaacca cacaatggca taccgcgatg
      781 gttctggcat atggaccatc tgtcggggtg ccacagtggt ggatggaaaa accgtttttc
      841 ccaatatgaa actgtcgaag gaaaaatgcg accaggtcaa cgccattgag cgtgataagg
      901 cgctggcatg ggtggagcgc aatattaaag taccactgac cgaaccacaa aaagcgggta
      961 tcgcgtcatt ttgtccctat aacattggcc ccggtaagtg tttcccgtcg acgttttata
     1021 agcggctgaa tgctggtgat cgtaaaggtg catgcgaagc gattcgctgg tggattaagg
     1081 atggcggacg cgattgccgc attcgttcaa ataactgtta cggtcaggtt attcgtcgtg
     1141 accaggagag cgcattaacc tgctggggga tagaacagtg aatcagatat tcatggtgat
     1201 ttttctcgtg ttgtcaggat ttatcgtcgg aaatgtctgg agcgaccgag gatggcaaaa
     1261 aaaatgggcg gaacgtgatg ctgccgcatt atcacaagag gtaaatgctc aatttgctgc
     1321 tcgaataatt gaacaggggc gaactatagc ccgtgatgag gctgttaaag atgcgcaaca
     1381 gaaatctgct gaaatttctg ccagggctgc ttatctgtct gatagtgtta accagttgcg
     1441 tgccgaagca aaaaaatatg ccatacgcct tgacgcagcg aagcataccg cagatcttgc
     1501 cgctgccgtc agaggcaaaa caaccaaaac cgccgaagga atgctcacca acatgctcgg
     1561 agatattgca gcagaagctc agctttatgc tgaaattgct gacgaacgct acatcgcagg
     1621 agtgacttgt caacagatct atgaatcttt aagagataaa aagcatcaaa tgtagggtaa
     1681 tattaaatcg gaacatttac atcgcggaat gtaaaattta aataaaaagg actcttccat
     1741 gagccaaaat tcctgaaatc ttaagggtaa gataaaaggt cttaatcaga atgacacgtt
     1801 ttattaataa ataaagctat tctttcattg ct
//
LOCUS       DAPHCV010000315         1791 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.320, whole genome shotgun sequence.
ACCESSION   DAPHCV010000315 DAPHCV010000000
VERSION     DAPHCV010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.320"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(376..531)
                     /locus_tag="RH033_004908"
                     /pseudo
     CDS             complement(376..531)
                     /locus_tag="RH033_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011637026.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            600..1762
                     /locus_tag="RH033_004909"
     CDS             join(600..861,861..1762)
                     /locus_tag="RH033_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HDV2993950.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     QLYNWRSKQQNQQTSSERELEMSTEIARLKRQLAERDEELAILPKGRDILREAPEMKY
                     VFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSK
                     QRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLL
                     EQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDAL
                     QMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFH
                     SLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENKNLA"
BASE COUNT          454 a          413 c          472 g          452 t
ORIGIN      
        1 aacacagtgt gtttatctta atcattcatg agggatatat gaaaggagga ggcgtagata
       61 ataataattc ttgttaaaga agtgagatgc tatgaaagat gaagaatatg tattgcgctt
      121 gttcattaga gtctctttat gtttttgtgt gttactattt cctgggtgag cagattgcca
      181 actcagcctc tgatagagga aattctgtga aaggacggga ttagtttgta tttttctttt
      241 gcatgctcag taaatgtatt caatatttat gcactgtttg ttgtcatttt tgcgtattac
      301 cgtgaaggag atcggtgagt aacatcgatg gagatcggtt cgtgtcactt tcacagaacc
      361 gtttttaaat taccttcact gatctccttc ggtcaacgga gattgtattt tccgcattga
      421 ttctccttta agttcgatct tgatactgcc atgaaccagt cgatctagga tggcatccgc
      481 atgtgtggag tctccgatca ttttgtacca gttttccacc ggcaactggc tcactgatct
      541 tacccagcaa tagtggacac gcggctaagt gagtaaactc tcagtcagag gtgactcaca
      601 tgacaaaaac agtatcaacc agtaaaaaac cccgtaaaca gcattcgcct gaatttcgca
      661 gtgaagccct gaagcttgct gaacgcatcg gtgttactgc cgcagcccgt gaactcagcc
      721 tgtatgaatc acaactctac aactggcgca gtaaacagca aaatcagcag acgtcttctg
      781 aacgtgaact ggagatgtct accgagattg cacgtctcaa acgccagctg gcagaacggg
      841 atgaagagct ggctatcctc caaaaggccg cgacatactt cgcgaagcgc ctgaaatgaa
      901 gtatgtcttt attgaaaaac atcaggctga gttcagcatc aaagcaatgt gccgcgtgct
      961 ccgggtggcc cgcagcggct ggtatacgtg gtgtcagcgg cggacaagga taagcacgcg
     1021 tcagcagttc cgccaacact gcgacagcgt tgtcctcgcg gcttttaccc ggtcaaaaca
     1081 gcgttacggt gccccacgcc tgacggatga actgcgtgct cagggttacc cctttaacgt
     1141 aaaaaccgtg gcggcaagcc tgcgccgtca gggactgagg gcaaaggcct cccggaagtt
     1201 cagcccggtc agctaccgcg cacacggcct gcctgtgtca gaaaatctgt tggagcagga
     1261 tttttacgcc agtggcccga accagaagtg ggcaggagac atcacgtact tacgtacaga
     1321 tgaaggctgg ctgtatctgg cagtggtcat tgacctgtgg tcacgtgccg ttattggctg
     1381 gtcaatgtcg ccacgcatga cggcgcaact ggcctgcgat gccctgcaga tggcgctgtg
     1441 gcggcgtaag aggccccgga acgttatcgt tcacacggac cgtggaggcc agtactgttc
     1501 agcagattat caggcgcaac tgaagcggca taatctgcgt ggaagtatga gcgcaaaagg
     1561 ttgctgctac gataatgcct gcgtggaaag cttctttcat tcgctgaaag tggaatgtat
     1621 ccatggagaa cactttatca gccgggaaat aatgcgggca acggtgttta attatatcga
     1681 atgtgattac aatcggtggc ggcggcacag ttggtgtggc ggcctcagtc cggaacaatt
     1741 tgaaaacaag aacctcgctt aggcctgtgt ccatattacg tgggtaggat c
//
LOCUS       DAPHCV010000316         1766 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.321, whole genome shotgun sequence.
ACCESSION   DAPHCV010000316 DAPHCV010000000
VERSION     DAPHCV010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.321"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(225..470)
                     /locus_tag="RH033_004910"
                     /pseudo
     CDS             complement(225..470)
                     /locus_tag="RH033_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937612.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(532..>1766)
                     /locus_tag="RH033_004911"
     CDS             complement(532..>1766)
                     /locus_tag="RH033_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDV2993951.1"
                     /translation="YPDRRRIFTSKANPVAPFEPEVKRQAVMALCTRQVSASEIARRI
                     GVSRAVLYKWKDKIIGNSAYQTMRKHNEPSLEAERDALREEVARLNQEIRRRQMELDI
                     LKKAEEIIKKDPGISISHLNNREKTKIADALRQTYPLTELLHVLGLARSSYFYHRAAL
                     KAGDKYATIRTMLTDIFNSNYQCYGYRRLHAMLRHEGGRLSEKVVRRLMVEEQLVVSR
                     NRRRRYSSYCGEIGPAPDNLIARDFKAEQPNQKWLTDITEFHLPAGKVWLSSVVDCFD
                     GKVVSWSLSTRPDAELVNTMLDSAVETLNAGERPVIHSDRGGHYRWSGWLERVNAEGL
                     IRSMSRKGCSPDNAACEGFFGRLKTEMYHGRKWSGITPEKFMQQVDTYIRWYNERRIK
                     LSPGAVSPKMYRQQCGLE"
BASE COUNT          399 a          482 c          387 g          498 t
ORIGIN      
        1 cttaaccaaa taatctaaca cttttaatcg acaggaatta aaaacccggg gaggttaaaa
       61 aaacacaaaa aaatggatgt ttattcaact ctggttacgt accatgagga ctgcatccac
      121 aatgcaccgg caattaaatg gctttcattg attgattttt tatatctgtg atgatttttt
      181 gctccattcc tcaaaaaatg gacgcaactt cccctctgcg gctctcagct gtatacccgc
      241 tgtcgtgcca gtggctcatg catcatgacc agctctgcca gacggtagcc ccgcttcagc
      301 cccgtaaacc gcatctgacc tccgcacagg atgcacttca gcgggtcaac cttcagtaac
      361 cgctgataca cccttctccg cctgatttgc atcgccgttt ttctctccgt ttctgttatg
      421 acgtacacaa cattttccag taaccggcgc ttcaccggac tcaggaaccc gttgtcaacg
      481 ccacgatgtt tgaccgttct ggggatgcgg acgatttcct ggactgcttt atcattccag
      541 cccgcattgt tggcggtaca ttttggggct gactgcaccc ggcgataatt ttatacgccg
      601 ctcgttatac catctgatgt aagtatccac ttgctgcatg aacttttctg gcgtgatgcc
      661 cgaccattta cgcccatgat acatttccgt tttcagtctg ccgaaaaagc cttcgcatgc
      721 cgcattatca ggtgaacatc ctttgcggga cattgagcga ataagacctt ctgcattcac
      781 tctttccagc cagcctgacc agcgataatg cccacctctg tcactgtgta tcaccggtcg
      841 ttcgccagca tttaacgttt cgacagcgct atccagcata gtgttgacca gttcagcatc
      901 ggggcgtgta ctgagagacc agctcacaac ttttccatcg aagcagtcca ccaccgatga
      961 tagccagact ttacctgcag ggaggtggaa ctccgtgata tctgtcagcc atttctgatt
     1021 aggttgctcc gccttaaaat ctctggcgat aaggttatcc ggagccggtc cgatttctcc
     1081 gcaatatgag ctgtagcggc gacgacggtt acggctgacg acaagctgtt cttccaccat
     1141 aagtctgcgt acaacctttt ctgatagccg ccccccctca tgcctgagca tcgcatgcag
     1201 gcgacgatag ccataacact ggtaattact gttaaatata tctgtcagca tcgtacgtat
     1261 cgtggcgtat ttatcaccgg ctttcagtgc agcccggtga taaaaataac tgctacgggc
     1321 aaggcccaga acatgcagta attctgtcag gggatatgtt tgtctcaggg catcagcgat
     1381 cttcgttttc tctctgttgt tcaggtgact gatactgatg cccgggtctt tttttatgat
     1441 ttcctccgcc tttttcagaa tatccagctc catctgccgg cggcgtattt cctgattcag
     1501 tcgggcgact tcctcccgca acgcatcgcg ttctgcctcc agggaaggtt cgttatgttt
     1561 acgcatagtc tggtaagcac tgttgccgat aattttatct ttccatttat acaataccgc
     1621 acgactgaca ccaatacgcc tggcgatttc actggctgat acttgtcgtg tacagagtgc
     1681 catgacagcc tgacgtttga cttctggttc aaagggcgca accgggttgg ccttactggt
     1741 aaatatacgt cgtctgtcgg gataaa
//
LOCUS       DAPHCV010000317         1763 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.322, whole genome shotgun sequence.
ACCESSION   DAPHCV010000317 DAPHCV010000000
VERSION     DAPHCV010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.322"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            106..1689
                     /locus_tag="RH033_004912"
     CDS             106..1689
                     /locus_tag="RH033_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDV2993952.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
BASE COUNT          400 a          426 c          487 g          450 t
ORIGIN      
        1 caaataatat gagataaata gtgctgcaac attgcatttt tgccccgatt tatccatgat
       61 cgaattgtga catttgtcat acaacgaata ggttttgtac ttactatgga atggattgcc
      121 gatccgtcta tctgggccgg gttaatcacg ctgattgtga tcgaactggt cctcggcatt
      181 gataacctgg tctttattgc catcctcgcc gaaaaactac cgccgaagca gcgcgaccgc
      241 gcacgggtta ccgggctgct gctggcgatg ttaatgcgcc tgttactgct ggcgtcaatc
      301 tcctggctgg tcaccctgac tcaaccgctg ttcagcttcc gctcgtttac ctttagcgcc
      361 cgcgacttaa tcatgctgtt tggtggtttc ttcctgttgt tcaaagccac gatggagctg
      421 aacgaacgac tggaagggaa agacagcaat aatccaacac aacgcaaagg tgcgaagttc
      481 tggggcgtgg tgacgcaaat tgtggtactg gacgccatct tctcactcga ctcggtgatt
      541 accgccgtcg ggatggtcga ccatttactg gtcatgatgg ccgccgtggt tatcgctatc
      601 agcctgatgt tgatggcaag caagccgtta acccagttcg ttaatagcca cccgacgatc
      661 gttattctct gcttaagctt cctgttgatg attggcttta gtctggtggc agaaggtttc
      721 ggcttcgtca ttccgaaagg ctacctgtac gctgccattg gtttctcggt gatgatcgag
      781 gcactcaatc agttggctat ctttaaccgg cgacgttttc tttccgctaa ccagacgctg
      841 cgccagcgga ctaccgaagc ggtaatgcgc ctgcttagcg ggcaaaaaga ggatgcggaa
      901 ctggacgccg aaaccgcgtc catgctggtg gatcatggta accagcagat ctttaatccg
      961 caggaacggc ggatgattga gcgggtactt aatcttaacc agcgtaccgt cagcagcatt
     1021 atgacgtcgc gccacgatat tgagcatatc gatctcaacg cgccggaaga agaaatccgc
     1081 cagttgctgg agcgaaatca gcatacgcgg ctggtggtta ccgacggtga tgacgcagaa
     1141 gatttgctcg gtgttgttca cgttatcgac ctgctacaac agtctctgcg cggcgaaccg
     1201 ctcaacctgc gggtgttgat tcgccagccg ctggtgttcc cggaaacctt gccgttgtta
     1261 ccagccctgg agcagttccg taatgcccgc acgcactttg cttttgtggt ggatgagttt
     1321 ggctcggtgg aagggattgt gacattaagt gacgtcactg aaaccattgc cggtaactta
     1381 ccgaacgaag tggaagagat cgacgcccgc catgatattc agaagaatgc ggacggttcc
     1441 tggacggcga atggtcatat gccgctggaa gatctggtgc aatatgtgcc gctgccgctg
     1501 gatgaaaaac gtgaatatca caccattgcc gggctgttga tggagtattt gcagcgtatt
     1561 ccaaaacctg gcgaagaagt tcaggtgggg gattatttgc ttaaaacgtt gcaggtggaa
     1621 agccatcgcg tgcagaaggt gcagattata ccgctgcgta aggatggcga gatggagtac
     1681 gaggtgtgat ggtatcggtc attttacccg atgctgcgta acgccttata acttcagcaa
     1741 tgaagcgttg tctgtggtac atg
//
LOCUS       DAPHCV010000318         1719 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.323, whole genome shotgun sequence.
ACCESSION   DAPHCV010000318 DAPHCV010000000
VERSION     DAPHCV010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.323"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(71..1420)
                     /locus_tag="RH033_004913"
     CDS             complement(71..1420)
                     /locus_tag="RH033_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HDV2993953.1"
                     /translation="MVSSLISGAHFLNDNHPWIPDTKSKIHDVCALDENGSYISDLIL
                     RLADAVNKRPDIKVWNLSLGGGPCNEQMFSDFAMELDRLSDKFGILFVVAAGNYVDEP
                     IRTWPNPDPLGGADLISSPGESVRALTVGSVSHMEANDALSEIGTPTPYTRRGPGPVF
                     TPKPDIIHAGGGVHRPWNVGASSLKVVGPDNRLCSNFGTSFAAPIVASLAAHTWQRIA
                     TNSDFNVSPSLIKALLIHSAQLSSPDYSPSERRYLGAGIPNEVIETLYDSDDRFTLIF
                     QTFLVPGVRWRKENYPIPSALIQNGKFKGEIVITAAYAPPLNPNAGSEYVRANVELSF
                     GLIENNTIKGKVPMEGENGQSGYERAQIEHGGKWSPVKIHRKAFNKGITSGNWALQAK
                     TTLRANEPALMEPLPVTIVVTLKSLDGNTQVYADGVRALNANNWAHYPLPARVPVSV"
BASE COUNT          522 a          379 c          309 g          509 t
ORIGIN      
        1 ggcggtattt ttataaaaca cacccacaaa taaatcaaac ccgctacagc gggtttgatt
       61 tatatagttg ttatacggaa actggcacac gagcaggcaa tggatagtga gcccagttat
      121 tagcatttaa agctcttaca ccatcagcat aaacttgtgt gtttccatct aatgatttta
      181 aagttactac aatagttaca ggtaaaggct ccattaaggc cggttcattc gctctcaacg
      241 ttgttttagc ctgaagagcc cagttacccg aagtaattcc tttattaaat gccttgcgat
      301 gaatttttac tggtgaccac tttccaccat gctcaatttg agctctctca tatccagatt
      361 gaccgttttc tccttccata ggcacttttc cttttatagt attattctca attaagccaa
      421 aacttagctc tacgttcgcg cgaacatatt cactgccggc attagggttt agtggtggtg
      481 catatgcagc agtaattaca atctcacctt taaattttcc attttgaata agtgccgatg
      541 gtatgggata gttttccttt ctccacctca ccccaggaac caagaatgtt tggaaaatca
      601 gagtaaacct atcatcacta tcatataagg tctcaataac ctcattagga attcccgctc
      661 ccaaatagcg tctttcactt ggcgagtaat caggagaaga taattgagcg gaatgaatta
      721 ataatgcttt aatcaatgat ggtgaaacat taaagtctga attagtggct attctctgcc
      781 atgtatgcgc agctaaactt gccacaattg gagcagcaaa actagtacca aaattagagc
      841 aaagcctatt atctggccct acgaccttta aactgcttgc tcctacattc caaggtctat
      901 gaaccccacc gccagcatgg attatatctg gctttggagt aaatacaggc ccagggccac
      961 gacgagtata tggtgtcggt gttccaattt cacttaaagc atcattagct tccatatgag
     1021 aaactgaacc aactgttagt gctcggactg actctccagg agaggaaatt aaatcagcac
     1081 ctccaagcgg atcaggattt ggccatgtac gtataggttc atctacataa ttacctgcag
     1141 caactacaaa caaaatacca aatttatcgc tgagccgatc taactccatc gcaaaatcac
     1201 taaacatctg ctcattacat ggtccgcctc ccaaagacaa attccagact tttatatctg
     1261 gtcttttatt tacagcatct gctagcctca gaattaaatc tgatatataa gatccatttt
     1321 catctaaagc acaaacatca tggattttag attttgtatc aggaatccat ggatgattgt
     1381 catttaaaaa atgagcgcct gatatcaatg aagacaccat agtcccatgt tcataactcg
     1441 tatcaggagg aattacgtat gtttccctac tcactaccca aggagtaatt gttgccgcga
     1501 tggggcttac cccagtgtca aatacagcaa caatgggtaa ttcttctgat ggtacgggaa
     1561 agctatttgt ttcaatgcca acagaatcac ttacactaac cgggaatgct gaatattttg
     1621 gttctggaat taacgttctt atacccgggt aatcaatgag aatgtctaat atatatctat
     1681 cacttgggga taaatccatt atccttaata agggaagac
//
LOCUS       DAPHCV010000319         1713 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.324, whole genome shotgun sequence.
ACCESSION   DAPHCV010000319 DAPHCV010000000
VERSION     DAPHCV010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.324"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..848)
                     /locus_tag="RH033_004914"
     CDS             complement(<1..848)
                     /locus_tag="RH033_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HDV2993954.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDTEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHP"
     gene            complement(909..1556)
                     /locus_tag="RH033_004915"
     CDS             complement(909..1556)
                     /locus_tag="RH033_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDV2993955.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFDRRIDLRVKTGAEAIRAL
                     AMQIPAFRQKLSDGWYQVRIAGRDAGETELSARLNEPLENGAVIHIVPRPAGAKSGGI
                     FQAVLGAALIAVAWWNPVGWLGATAVSGMYAAGASMILGGVAQMLAPKARTPTPASTD
                     NGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(1454..>1713)
                     /locus_tag="RH033_004916"
     CDS             complement(1454..>1713)
                     /locus_tag="RH033_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDV2993956.1"
                     /translation="YQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLS
                     KRERYTDKWQRRTHSLWRHREWHASAFTGICNDLTAASIFV"
BASE COUNT          348 a          547 c          438 g          380 t
ORIGIN      
        1 ggatgggtca gcatatccca cagacaccag gccatgttgt tgctgtatgc tggcttaaac
       61 gttccgtccc agataccgct gtattgccgc gtctgcgggt tatagttcga cggcacctgc
      121 agaatgcgcc cgcgcagatg ataattacgg ctcacctgct ggctgccgaa ctgctccgaa
      181 tccacctgca cgccgaccag tgccgtgttc gggtagcact gtttcacatc gatgatttcg
      241 gtgtatgacg accagagcgt tttgttctgc agctggtctg tggtgctgtc cggcgtcatc
      301 ctgcgcatcc gtatattgaa cgggcgcggc ggcaggttac ccaccaccac cgaggccaga
      361 tactgcgagg tggttttgcc cttaatggtg atgtcttttt ccgtcaccca gccaccatta
      421 cgctggatct gaaccagcag gcggacttcc gacggattcc ggtccccctt tgaggtggtt
      481 tccaccagtg cctgcacgcc gaaggtaaag cgcagacggt cgatgtttgc agacgtaatg
      541 gtgcgggtga tcggcgtgtc atatttcact tccgtaccca gcaccgtctc ggagccggag
      601 gattcaaacc cctccggcgg tgtctgctcc tgctcaccgg cacggaacac caccgtgaca
      661 ccggagatgt tggtattccc ctcagtgtcc agcaccggcg tactgttcag cagcacgctt
      721 tttaatccgt ccaccggacc ttcaaccggc ccttcgctga tggcatcgat cacactcagc
      781 agctgcgtgg atttcaggtt atccttcgct tcgcgcgggg tatgcccctt actgctgcct
      841 ttacccattc gtcatgctcc ataaacgaca aaaccgcccg gaggcggttt cacataaaac
      901 attttgcatc agcgaccaat caccacaacc tgaccaccgt ccccttcgtc tgccgtgctg
      961 atctcctgag aaaccacccg cgaccccacg cgcatttcac cgtacagaac gggcagaaca
     1021 ttgccctggg caaccatgtt atccagtgac gagaaatacg tgttctgttt gccgttatct
     1081 gtactggctg gcgtgggcgt cctggctttc ggtgccaaca tctgcgccac accgcccagg
     1141 atcatactgg cccctgccgc atacataccc gatacagccg tggcccccag ccagcccaca
     1201 gggttccacc atgcaaccgc aatcagcgcc gcccccagca ctgcctgaaa aataccgcca
     1261 cttttagcac ccgccggacg cggtacgatg tggatcacgg caccattttc cagcggctca
     1321 ttaagacggg cagacaattc agtttcacct gcatcacgcc cggcaatgcg tacctggtac
     1381 cagccgtcgc tcagtttctg acgaaatgcc gggatctgca tggccagcgc ccggatagct
     1441 tcagcccccg ttttcacacg aagatcgatg cggcggtcaa atcgttgcaa atccccgtaa
     1501 aggcagatgc gtgccattcc cggtgacgcc agagggagtg tgtgcgtcgc tgccatttgt
     1561 cggtatacct ctctcgtttg ctcagttgtt caggaatatg gtgcagcagc tcgccatcac
     1621 cacagtaaat ggcggcatga ttcggcaccg atgaaccaaa acagcacagc agcacatcgc
     1681 ccggttgtgc tgatgacaac ggcacctgat aca
//
LOCUS       DAPHCV010000320         1698 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.325, whole genome shotgun sequence.
ACCESSION   DAPHCV010000320 DAPHCV010000000
VERSION     DAPHCV010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.325"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1013)
                     /locus_tag="RH033_004917"
     CDS             complement(<1..1013)
                     /locus_tag="RH033_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDV2993957.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPYLAVKFHLFTDYIDEDYLQRVNAFTNKNANVEVRIYKVSNAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKSRHLKRNGKIASSFYYFMLYYSKK
                     IAR"
     gene            complement(1039..>1698)
                     /gene="rfaY"
                     /locus_tag="RH033_004918"
     CDS             complement(1039..>1698)
                     /gene="rfaY"
                     /locus_tag="RH033_004918"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HDV2993958.1"
                     /translation="FYYKDNDNKYKEIFDEILAYNFKTVKVLRNIDDTKVSLIDTKYG
                     RYVFKVFAPKTKRNERFFKSFVKGDYYQNLIVETDRVRSAGLTFPNDFYFLAERKIFN
                     YASVFIMLIEYVEGVELNDMPIIPENVKAEIKASMEKLHALNMLSGDPHRGNFIVSKD
                     GVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTKLRKFIKKLKGK
                     A"
BASE COUNT          535 a          340 c          284 g          539 t
ORIGIN      
        1 ttacgcgcta ttttcttact ataatagagc atgaaatagt aaaatgaaga tgcaatttta
       61 ccatttcttt tcaaatgtct ggatttcact tgatactgct tttcattcgt ggctggaata
      121 aacgccacat cattccagca agaagcctga taagcctcat taaaatactt ctggcaggga
      181 taattcgtcc agtcgttcca gggcttcgtt acgccaacat aatgaatcag cttagtatca
      241 tcggtaatgg tctttttata atcttcatcg cttttatttt ttaactcata gtcaatgcca
      301 taaaagcaat cataatcgcc gctaataaaa atacaatgac caaagaacag aatatttaaa
      361 atatcctgat caaggcattt atatttcgtg tgctgcgggt cacttgccag catagcaatg
      421 gctttattca taaaatcatt tttcgcccat gcttcaagct gcagatatac tacaccggcg
      481 ttgaaataat tccctggaag tccctctata gccagacgct tcgctggttt ttcctgcata
      541 aaaggcacat ctttaataac agccgcgaac ttatcgccat caaaattaat atcaagcaat
      601 ccagctaatg agcctttaca gatgacatca gcatcgatat ataacagatt ttcaatagtt
      661 tcactcaaat actggaacgc aactaaacgg aagaatgttg cataagacca ctgtttcagg
      721 ctggggaaga tatcaataaa ggcattggag actttataaa ttcttacttc aacgttagca
      781 tttttgttgg taaaagcatt aacacgttgt agataatctt catcaatgta gtcagtaaag
      841 agatgaaact taaccgcaag gtacgggtta tgcataacaa ctgattgcat agatactgcc
      901 gcaccaaata aaaagttgcg gtcaataccg taagcgatat ttaacacacg atcggtgtta
      961 gcaatttccc cagagacagc gacttcagta atactctcag ctaataaatc catgttggtt
     1021 ccgttttgac tgtgtggttt acgctttgcc ttttaatttt ttaataaatt tgcgtagttt
     1081 ggttctgtag attactgaat agtagccata atcttttatt tcgttggcaa tacccaggtg
     1141 acgttccatg gccagacgat cacgagcttt acgctctgcc gtacagcttt taccggatag
     1201 gtcaattatc ctgacaccat ctttactaac gataaagtta ccccggtgag ggtcgcccga
     1261 tagcatattt aaggcatgca atttttccat tgaagcctta atttctgctt taacattttc
     1321 tggaataatc ggcatatcat tgagttctac gccctcgaca tactcgataa gcatgataaa
     1381 aacactggca tagttgaaaa ttttacgctc agccagaaaa taaaaatcat taggaaaagt
     1441 aagacctgca cttctgacac gatcggtttc aacgatcaaa ttctgatagt agtcaccttt
     1501 tacaaaagat ttaaaaaaac gttcattcct tttcgttttg ggagcaaaga ctttaaaaac
     1561 atagcgacca tatttcgtat caatcaacga tactttggta tcatcaatat tacgtaatac
     1621 ttttactgtt ttaaaattat aggccagtat ttcatcaaag atttctttat atttattatc
     1681 gttatctttg taataaaa
//
LOCUS       DAPHCV010000321         1653 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.326, whole genome shotgun sequence.
ACCESSION   DAPHCV010000321 DAPHCV010000000
VERSION     DAPHCV010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.326"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..594
                     /gene="traW"
                     /locus_tag="RH033_004919"
     CDS             <1..594
                     /gene="traW"
                     /locus_tag="RH033_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HDV2993959.1"
                     /translation="QSVSAADLGTWGDLWPVKEPDMLTVIMQRLTALEQSGEMGRKMD
                     AFKERVIRNSLRPPAVPGIGRTEKYSSRLFDPSVRLAADIRDNEGRVFARQGEVMNPL
                     QYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEMQKSLDSRVYFDQN
                     GVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            591..1583
                     /locus_tag="RH033_004920"
     CDS             591..1583
                     /locus_tag="RH033_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HDV2993960.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSIKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
BASE COUNT          371 a          427 c          489 g          366 t
ORIGIN      
        1 cagagtgtgt ccgccgccga tcttggcacc tggggcgatc tgtggccggt aaaggagccg
       61 gacatgctga cggtgatcat gcagcgcctg acggcactgg agcagtccgg tgagatgggc
      121 cggaaaatgg atgcctttaa ggagcgggtg atccgcaaca gtctgcggcc ccctgccgtg
      181 cccggtatcg gacgcacgga gaaatacagc agccggttgt ttgacccgtc cgtcagactg
      241 gctgcggata tccgggataa cgaagggcgg gtgtttgccc gtcagggtga agtgatgaat
      301 ccgctgcagt atgtgccttt taaccagacg ctgtatttca tcaatggcga tgatccggcg
      361 caggtggcct ggatgaaacg ccagacgccg cccacactgg agagcaaaat tatcctcgtg
      421 cagggcagta tcccggagat gcagaagtct ctggacagcc gtgtttactt tgaccagaac
      481 ggtgtgctct gccagcgcct cggcattgat caggtcccgg cacgggtgag cgccgttccc
      541 ggcgatcgct tcctgaaggt ggaatttatt ccggcagagg agggcagaaa atgaagcgaa
      601 gcctgtggct gctgatgtta ttccttctcg ccggtcatgt ccctgcggca tctgcggatt
      661 ctgcctgtga ggggcgtttt gtaaacccga tcacggatat ctgctggagc tgtattttcc
      721 cgctctcgct gggcagtatc aaagtcagtc agggtaaggt ccccgacacg gcgaacccgt
      781 cgatgcccat tcagatttgt ccggcaccgc cgccgctgtt caggcgtatc gggctggcca
      841 ttggttactg ggagccgatg gcgctgacgg acgtcacccg gtcaccggga tgcatggtga
      901 acctgggctt cagcctgccg gcttttggta aaacggcaca gggaacggcg aaaaaggatg
      961 agaagcaggt gaacggggcg ttctatcacg ttcactggta caaatacccg ctgacgtact
     1021 ggcttaacat catcacatcg ctgggctgtc tggaaggtgg tgacctggat atcgcttatc
     1081 tttctgaaat tgaccccacc tggacggaca gcagcctgac caccattctc aatccggaag
     1141 ctgtcatctt tgccaatccg atagcacagg gggcctgtgc agcagatgcc attgccagcg
     1201 cctttaatat gcctctcgat gttctgttct ggtgtgccgg ttcgcagggc agtatgtacc
     1261 cgttcaatgg ctgggtgagt aatgagtcca gtccgttgca gtcctccctg ctggtcagtg
     1321 aacgcatggc gttcaaacta caccgtcagg gcatgattat ggaaaccatc gggaaaaata
     1381 acgccgtctg taatgaatat ccgtccccaa tcctgcctaa agaacgctgg cgttatcaga
     1441 tggtgaatat gtatccggac agcggacagt gccatccgtt cgggcgcagt gtgatgcgct
     1501 gggagacggg aaaaaatccg cccaacacaa agaaaaactt cggctacctg atgtggcgta
     1561 aacgtaactg tgtattcctg taaatcattt taatgaataa gaggaaatga aaatggcaac
     1621 aatggacagt aaatatattc agcacctcag gaa
//
LOCUS       DAPHCV010000322         1606 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.327, whole genome shotgun sequence.
ACCESSION   DAPHCV010000322 DAPHCV010000000
VERSION     DAPHCV010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.327"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            133..804
                     /gene="queE"
                     /locus_tag="RH033_004921"
     CDS             133..804
                     /gene="queE"
                     /locus_tag="RH033_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HDV2993961.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          448 a          345 c          370 g          443 t
ORIGIN      
        1 ttactccctt ttattattaa cgatctgttt ctcatttttc tttaaacctg cctgtaacag
       61 aatgttaata agacatgatt gttgtggcag gatctgcaat aattaacaat tatccacttc
      121 acagagaatg ctatgcagta cccgattaac gagatgttcc agaccctgca aggtgagggt
      181 tactttaccg gcgtccccgc catttttatt cgtttacagg gatgcccggt tggctgtgcc
      241 tggtgcgaca ccaaacacac ctgggaaaag cttgaggatc gggaagtctc ccttttcagc
      301 attctggcga agaccaaaga gagtgataag tggggggctg cgagcagtga agatttgctg
      361 gctgtcattg gtcgccaggg atacaccgca cggcatgtgg tgattacggg tggtgagcct
      421 tgcattcatg atttgctgcc actgactgat ctgctcgaaa agaacggttt tagctgccag
      481 atcgaaacca gtggtactca tgaggtacgc tgcacaccga atacctgggt taccgtatca
      541 ccaaagttga acatgcgcgg cggctatgaa gtgttgtcac aggcactgga gcgagccaac
      601 gaaatcaagc atccagtggg gcgcgtacgt gatattgaag cactggatga actactggca
      661 acgctgaccg atgataaacc gcgagtcatt gcactgcagc cgattagcca aaaggatgat
      721 gccacacgtt tgtgcattga aacctgcatt gcgcgtaatt ggcgtttgtc gatgcaaaca
      781 cataaatatc taaatattgc ctgattaaac atttataagc gttataaatg ggtggaacct
      841 gtaaagactt ctactcattt atattgtttg tcgcctctga aaactcatcc atcttgccca
      901 tcctgaacta atcattatca ttctctacaa attctgtggc gttaattttt cgctggagtg
      961 aaaattattg cggtaaagtt tggtagattt tagtttgtat agagttattt taaatattta
     1021 cctttttaat caatggatta agtgctcttt aacataatgg atgtgttgtt tgtgtgatac
     1081 tgtaaagttg gtagattgtg actggcttaa aaaatcatta attaataata ggttatgttt
     1141 agagagttcc ccacgtcagc ggggataaac cgacgtaaca aaacaacagc aaaatattat
     1201 cgacgagttc cccacgtcag cggggataaa ccggctccgc cggtttgatc tccggtttgc
     1261 gctgtgagtt ccccacgtca gcggggataa accgctagga taaattaaaa gacaaaattg
     1321 cagcaagagt tccccgcggc agcggggata aaccggagcg accagtatca agatcgacag
     1381 gttttgcgag ttccccgcgc cagcggggat aaaccgatcg atatgtacgt tagcgagggg
     1441 atcacgcaga gttccccgca ccagcgggga taaaccacca tataacccgt tatctctttc
     1501 tcaagttttt atattagctg tacttgtaat aagcaacata tccacataac acctcatgtt
     1561 caaaatagtt ctccatgcca gataagttca caattatcga tacaaa
//
LOCUS       DAPHCV010000323         1601 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.328, whole genome shotgun sequence.
ACCESSION   DAPHCV010000323 DAPHCV010000000
VERSION     DAPHCV010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.328"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>1601)
                     /locus_tag="RH033_004922"
     CDS             complement(<1..>1601)
                     /locus_tag="RH033_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993962.1"
                     /translation="LSSLSSLVVVNDPKKMKRIFPLFIVSENINIHAEPDIEYFRVID
                     RAFDEVANYTGRIFSYLRTHGPLKLTSCVNKQTLLSMGGYLNEWNVFDSLSRVRDFFR
                     LSSAVFTKLDNNIYSLEVDSFCLYRDYEIARNRLMMRASHLYSEVHEFSNKHFHLNSW
                     VKDHMPSYLNSDGVFSSFHLSELENMSPDDLHEEYGNISLFNWVHAYQCLVELSKEEM
                     SKRFSSTKPIPLQLDRWLIIKSRESWLSFFQRKGIAADAAKKLIDYFTFNSKSHDLND
                     CPFIPCMDGLCLMPALIANSSVTRSLMSLFGSKKISQASKGRFHEQQFIKQVRDAGIK
                     ASPIDAHANYQCDCVILLDDCLIFTELKSNGQPIYYGKYYQQVYNIVGDSSLIHDHNN
                     KFMRSYFQQINRISEHYLNHLDVIIKEFELPSTWQPKGVYKLIVTTTMLGGKYHVDDI
                     YVADKYALSSFFQRIPGVIYQTNENGKMAKNIIDGFECCEGEITIDKFIDYLSSLPSI
                     NAVRKNIKKLTYSVRFNEKLAHHPY"
BASE COUNT          535 a          287 c          247 g          532 t
ORIGIN      
        1 aataagggtg atgggctaat ttttcattaa atcgaacact ataagttaat ttttttatat
       61 ttttacgtac agcattaata ctgggcaaag aggaaagata atctataaac ttatcgatag
      121 tgatttcccc ttcgcaacac tcaaacccat caatgatatt tttcgccatt tttccatttt
      181 catttgtttg atatatcact ccaggtattc gttgaaaaaa actagataat gcatatttgt
      241 ccgctacata tatatcatcc acatgatatt tcccaccgag catagttgta gtaacaataa
      301 gcttatacac acctttgggt tgccatgttg atggtagttc gaattcttta atgatcacat
      361 caagatgatt aagataatgt tcagaaattc gatttatttg ttgaaaataa gaacgcataa
      421 atttattatt atgatcatgt attaatgagc tatctccaac gatgttatac acttgctgat
      481 agtacttacc ataatatatt ggttgaccat ttgattttag ttctgtaaaa atcaaacaat
      541 catccaggag aatcacgcaa tcgcactgat aattagcatg agcatcaatt ggagaagctt
      601 taatccccgc atcacgaact tgttttataa attgttgttc atgaaatcgc cctttacttg
      661 cttgagaaat ttttttggaa ccaaataatg acattagaga gcgagtcact gagctatttg
      721 ctattaatgc aggcattaag cataaaccgt ccatacaggg aataaatggg caatcattaa
      781 gatcatgtga ttttgaattg aaggtaaaat aatctatcag tttttttgct gcatctgctg
      841 caatcccctt acgttgaaag aatgataacc aactctcacg gctttttatg atcagccagc
      901 gatccaactg taggggtata ggttttgtcg atgaaaatct tttgctcatt tcttctttag
      961 ataactcgac taaacattga taagcgtgca cccaattaaa taatgatata ttcccatatt
     1021 cttcatgaag gtcatcagga gacatatttt ccaactcgct tagatgaaat gagctaaaaa
     1081 cgccatcaga atttaagtaa gatggcatat gatcctttac ccaactattt agatgaaaat
     1141 gtttgttact aaattcatga acttcgctat atagatgtga cgctctcatc attagtctat
     1201 ttcttgcaat ctcataatct cggtataaac aaaaactatc aacctcaaga gagtaaatgt
     1261 tattatccaa ttttgtaaat actgcactac ttagtctgaa aaagtctctt actctgctta
     1321 atgagtcaaa aacattccac tcattaaggt accctcccat tgaaagcaag gtttgtttat
     1381 tcacacaaga agttagtttc aatggcccat gagtccgtaa ataagaaaat attcttccgg
     1441 tgtaattggc tacctcatca aatgccctat cgataactcg aaaatattcg atatcaggtt
     1501 cagcatgaat attaatattt tcagaaacga taaataatgg aaaaatcctc ttcatttttt
     1561 taggatcatt gaccacaacc aaggatgata atgatgataa a
//
LOCUS       DAPHCV010000324         1567 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.329, whole genome shotgun sequence.
ACCESSION   DAPHCV010000324 DAPHCV010000000
VERSION     DAPHCV010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.329"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            57..788
                     /gene="bluR"
                     /locus_tag="RH033_004923"
     CDS             57..788
                     /gene="bluR"
                     /locus_tag="RH033_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HDV2993963.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            1009..1413
                     /locus_tag="RH033_004924"
     CDS             1009..1413
                     /locus_tag="RH033_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HDV2993964.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLADAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            1466..>1567
                     /locus_tag="RH033_004925"
     CDS             1466..>1567
                     /locus_tag="RH033_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993965.1"
                     /translation="MEIKVQRISLWMINTVFLLPPINNHQANTINLIF"
BASE COUNT          470 a          336 c          346 g          415 t
ORIGIN      
        1 aaagtattat tgggtcgtgt acaggcgacg gagatttgtg accgcaagga ggaattgtgg
       61 cttattacag cattggtgat gttgctgaac gttgcgggat taatcctgtc actctccggg
      121 cctggcaacg ccgctacggt ttgttaaaac cacagcgcag tgaaggcgga caccgactct
      181 ttgatgaaga agacatacaa cgcatcgaag agatcaagcg ttggataagt aatggcgttc
      241 ctgtaggcaa agttaaagca ttactggaaa ccaccagcca ggatacggaa gatgactgga
      301 gccgcctgca agaagagatg atgtcaattc ttcgtatggc taatcctgcc aaactacgcg
      361 cgagaattat ttcactgggt cgagagtacc cggtagatca attgattaat catgtttacc
      421 ttcctgttcg ccagcgtctc gtgcttgatc acaacacctc ccgcattatg agcagtatgt
      481 ttgacggcgc attaattgaa tacgcagcag catcgctttt cgaaatgcgc cgtaagcccg
      541 gtaaagaagc cattctgatg gcgtggaatg ttgaagagag agcacgattg tggctggaag
      601 catggcgttt atcattgtca ggatggcaca tttctgttct tgctgatccc attgaatcgc
      661 cgcgcccgga actgttccca acgcaaacat tgattgtctg gacaggcatg gcaccaacga
      721 gaaggcaaaa cgaactttta caacactggg gtgagcaagg ttataaagtc atctttcatg
      781 ccccctaatt tcacctttat cccgcccctt agacagaatc ttataatctg aacccacaac
      841 agcgtttgca ccggagctgg tccgttgcaa aatgctcacc gggagaattc aggtaatccc
      901 cctttcacac tttgtacata tcacagccaa aggacagacc agattgaagt ggtaaaaccg
      961 atcgtgcaca ctacgcagtc tcacaactaa caagaggtta ttatcctgat ggatcaggtc
     1021 gttgtttttc aaaagatgtt tgagcaagtg agaaaagagc aaaacttctc ctggttttat
     1081 tcagaattaa aacatcaccg tattgcacat tacatttatt atctggctac ggataacatc
     1141 agaattatta ctcacgatga cacggttttg ttattaagag gaaccaggaa cctgttaaaa
     1201 gttagtacga ccaagaaccc tgctaaaata aaagaggccg cattgcttca tatttgcgga
     1261 aaatctacat ttcgggagta ctgttcaaca ctggcagacg cgggcgtttt ccggtgggtt
     1321 actgatgtta atcataacaa acgcagttac tacgccattg ataatacgct tttatatatt
     1381 gaagatgtag aaaataataa accattaatc tagctaaagt tggatgctta aaaaatgctt
     1441 cataattcag taaggcatta gcataatgga aataaaagtg cagagaatat ccctatggat
     1501 gattaatact gtctttttat tgccacccat aaataatcac caggccaata ctatcaactt
     1561 gatattt
//
LOCUS       DAPHCV010000325         1552 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.330, whole genome shotgun sequence.
ACCESSION   DAPHCV010000325 DAPHCV010000000
VERSION     DAPHCV010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.330"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(924..1400)
                     /locus_tag="RH033_004926"
     CDS             complement(924..1400)
                     /locus_tag="RH033_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV2993966.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEELEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          346 a          418 c          389 g          399 t
ORIGIN      
        1 aaaaaaacaa caacttacaa atcggcgtaa aagatctatt ctaggcaagg gtgttgcgcg
       61 caacgcaacg ccctttgggg gtgtcggggc gcagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctctgcaag cctcgccagc cgtgattttt gccagatttc
      181 gtggttttca cgttcaacag ctcctgcacg ttttttgccg tccgttcacc gttaaactcc
      241 gcagaaatcg ggacaggatg tgcaaatgta ataccgtcac ccgcgacgat attccgtttg
      301 ccatctggtc agggcttcgc cccgacaccc cgtatcagtg cgtacgtgct caatgtggtt
      361 tccggttctc ttccgcttcc tcgcccactc gctcactaac gttcggtcgc tggggctgcg
      421 gcgagcggtg ccacctctcg catgttgtcg gtgattccgc tgttgttctt tcggggatga
      481 gtcgtcagat agcccttcaa actcgctcag aagctctcag acgcacgaga cggggtttaa
      541 atgcccgtca gggcatggaa ggcgcgaaaa acgcttacag agcgtttcag gaggtcaggc
      601 gcgccgatac ccctagccag gggctttcag gtcgccagga accgcgccag ccccctgcaa
      661 agcccatttg cacggtacag gcagccactc ggttggtagg tggtcgtaga ccgtcggtgt
      721 tacagcgatg ggggtctggg gttctcccca gacggtttcc ggttgcgctc aaggcgctgc
      781 cggaaccgtg gcggctcggt cgttctggag atcggggagc aaaaacacgc aagggatttt
      841 gaggcggcaa aagagcaatg caggatagac aatcatggtc agaacgttaa cacgcagccc
      901 ctgattgtcc gcctgccgct ggctcaattt gaaaacatgg ccatgattcg gcgctgaata
      961 tcaccgtctt tgttgaccag tgttttagtg acaaacgctg catggttttt cgtagtgtta
     1021 tatcccttcc agacttcatg tggatcgaga gcatacgcac aaaccttacc agcaacgggg
     1081 attctaacta atgcattttt tgactctaag taattgagcc accgaataat tgtgcttctg
     1141 ctaactccaa gtttttcagc taggaattgc tgatccgcaa cgacagcgcc acacgtagga
     1201 tcgatatgct ccgctaaaaa tgagtacagc ttcagtgctg atatgccttg gctatccttc
     1261 agcagctctc gaacgcgctc ccaacctttt ggggatacct gtgtaaacct tgaattcttc
     1321 tttgcttctt cgagctcctc ggtgtttttc tttgcctcat ctgaaagttg tttcagtcgt
     1381 ttttctgaat caataaacat gattttttcc ctctgaaaat cccatgcact aacgttggtt
     1441 caaatctaag ccatcatgac acatctgtgc aacaccttgt tgcgtgttgt cgcatccagg
     1501 atgcgacaac gttgtgtcac cctagatgcg acaggtaaac aaaaaacaca ct
//
LOCUS       DAPHCV010000326         1545 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.331, whole genome shotgun sequence.
ACCESSION   DAPHCV010000326 DAPHCV010000000
VERSION     DAPHCV010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.331"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..376)
                     /locus_tag="RH033_004927"
     CDS             complement(<1..376)
                     /locus_tag="RH033_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HDV2993967.1"
                     /translation="MSFSHSSLSAQVKSYLTFLPEEIRQKILEHLHGVIHYEPVIGIM
                     GKSGTGKSSLCNAIFQSRICATHPLNGCTRQAHRLTLQLGERRMTLVDLPGIGETPQH
                     DQEYRALYRQLLPELDLIIWILR"
     gene            561..>1545
                     /locus_tag="RH033_004928"
     CDS             561..>1545
                     /locus_tag="RH033_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2993968.1"
                     /translation="MTGRKNKCVRKNCNNQVFSVKMAVEKRLCRKHYERFCELKKRRE
                     QNPVHRLNEALPRKIKGPKSKKKLVKEREIINSRKKHKQEQGPSLTELNKKVREILKT
                     PAWRTLETRIRRNGITFKSKAELDVLSELYAIYSWVSYCKRSDNYSVWFLNCPDVNIN
                     LHVCECRSSESDCYLPSRFIILPAPLRQKVKRKYSANSSGVSGHNDSKSLYKTVLSVC
                     GGKRETELFFYSLPVKTNHHPYWIKSGVVKRFPPVFGLLFYEQERLTGGVFRYVLIRF
                     YNTFKENLFFVEIVSCCFLVELFHDIDSFFINQRLSKLESFIDKWREGKEIW"
BASE COUNT          494 a          240 c          368 g          443 t
ORIGIN      
        1 cccgcaggat ccagataatc agatccagtt ccggcagtaa ctgacggtaa agcgctcggt
       61 attcctgatc atgctgcggt gtttcaccaa tgccgggcag atcgaccagc gtcattctgc
      121 gttcaccgag ctgcagggta agacgatgag cctggcgggt gcagccgttc aggggatgcg
      181 tggcgcagat acgggactga aaaatggcat tacacagact gcttttgccg gtgccggatt
      241 tacccataat gccaatcacg ggctcgtaat gaataacacc gtggagatgt tcaaggattt
      301 tctggcgaat ttcttcagga agaaacgtca gatatgattt tacttgtgca gagagggaag
      361 aatggctgaa tgacataccg gtatcctgtt gatttcgctg aaccggatca cactaatccg
      421 gtttatcatt caggtaatat ggacgtgaaa aatgatggaa tgtctgaatt acggacatga
      481 ttgttaaagt gtaatggagt atgaagtgag tttttccgaa cgggaactaa tgacatactg
      541 cggtttcagg agatgagagg atgaccggac gtaaaaacaa atgtgtcaga aaaaattgta
      601 acaatcaggt tttctcagta aaaatggcag tggagaaaag actgtgcaga aaacattatg
      661 agaggttttg tgagctcaaa aaaagaagag agcaaaatcc tgttcacaga ttaaatgaag
      721 cattgccaag aaagataaaa ggaccaaaaa gtaagaaaaa attagtaaaa gaaagagaaa
      781 tcattaacag cagaaaaaaa cataaacagg agcagggacc atcgctgact gaacttaata
      841 aaaaagttcg tgagatactg aaaactccgg catggagaac gctggaaacg agaattcgaa
      901 gaaacggtat cacatttaaa tcaaaagcag agttagatgt actttctgaa ctttatgcaa
      961 tttattcatg ggtttcatat tgcaaacgga gcgataatta ttctgtgtgg tttttgaatt
     1021 gtccggatgt aaatataaat cttcatgtgt gcgaatgccg gtcttctgaa agcgattgtt
     1081 atctgccatc ccgttttatt attcttccag ctccattaag gcaaaaagta aaaagaaaat
     1141 actctgctaa ttcgtctggg gtatcaggac ataatgattc aaaaagtctt tataagacag
     1201 ttttgtctgt ttgtggtggg aaaagagaaa ccgaattgtt cttttattca ctgccagtaa
     1261 agactaatca tcatccttac tggattaaaa gtggtgtggt taaaagattt cctcctgttt
     1321 ttggtttgtt gttttatgaa caggaaagat taacaggagg tgtttttcgt tatgtgttga
     1381 taaggtttta taatactttt aaggagaatt tgttttttgt tgagattgtc tcgtgctgtt
     1441 ttcttgttga gctttttcat gatattgatt cgttttttat aaatcaacga ttaagtaagc
     1501 tggaaagttt tatagataag tggagggagg gtaaagaaat ctgga
//
LOCUS       DAPHCV010000327         1531 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.332, whole genome shotgun sequence.
ACCESSION   DAPHCV010000327 DAPHCV010000000
VERSION     DAPHCV010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.332"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..711
                     /locus_tag="RH033_004929"
     CDS             <1..711
                     /locus_tag="RH033_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDV2993969.1"
                     /translation="WLMMYGSISSAFRAPTMAEMYRDDVHFYRKGKPNYWVPNLNLKP
                     ENNITREIGAGIQLDGLLTGNDRLQLKGGYFGTDARNYIATRVDMKRMRSYSYNVSRA
                     RIWGWDMQGNYQSDYFDWMLSYNRTESMDASSREWLGSGNPDTLISDISIPVGHGGVS
                     AGWRAELSAAATHVKKGDPHQAGYAVHSFSLSYKPVSVKGFEASVTLDNAFNKLAMNG
                     KGVPLSGRTVSLYTRYQW"
     gene            718..1509
                     /locus_tag="RH033_004930"
     CDS             718..1509
                     /locus_tag="RH033_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HDV2993970.1"
                     /translation="MMKNTGYILALCLTASGHVLAHDVWITGKQAENNITAEIGYGHN
                     FPSKGTIPDRRNFFENPRIYNGKETITLKPASTDYVYKTESASKDNGYVLSTYMKPGY
                     WSRTSSGWKPVSREGRNDVAYCEFVTKYAKSFIPGVQQMPAQLYQSPTGHELEIIPLS
                     DISRFSEDVKLQVLYKTSPLAGAIMELDSVSYLKSSRHTHAVEHKHPVHKAELTFVTN
                     EDGIVTVPSLHIGQWLAKVQNKKSFQDKSLCDETVDVATLSFSRN"
BASE COUNT          432 a          339 c          361 g          399 t
ORIGIN      
        1 tggctgatga tgtacggctc catatcctct gcattccgtg cccccacaat ggcagaaatg
       61 tacagggatg atgtacattt ttaccgcaag ggtaaaccca attactgggt acctaacctt
      121 aatctgaaac cagaaaataa cattacccgt gagattggcg caggtattca actggacggc
      181 ctgcttacag gcaatgatcg gctgcagttg aaaggcggat atttcggaac ggatgccaga
      241 aactatattg ccacgcgcgt ggatatgaaa cggatgcgtt cttattctta taatgtatcc
      301 cgggcccgta tctggggatg ggatatgcag ggaaattacc agtctgatta ttttgactgg
      361 atgctttctt ataaccgcac ggaaagtatg gatgccagca gcagggaatg gctgggctcc
      421 ggcaatcctg acacactcat cagtgacatc agtatacctg ttggtcatgg aggagtttct
      481 gccggatggc gtgctgaact ttcggcagca gccacgcatg tgaaaaaagg cgatccccat
      541 caggctggct atgccgtaca ttccttttca ctgtcttata agcctgtaag tgttaaaggc
      601 tttgaggcat cagtgactct ggataatgcc ttcaacaagc ttgccatgaa tggcaaagga
      661 gtgccgcttt caggcagaac tgtcagtctt tatacccgtt atcagtggta acaaatcatg
      721 atgaaaaata caggttatat cttagctctt tgtctgacag catcggggca tgtcctggcc
      781 cacgatgtct ggattacagg taaacaggca gagaacaaca ttaccgcaga gattggttat
      841 ggtcataatt tcccctcaaa gggcacaatt cctgacagaa ggaatttctt tgaaaatccc
      901 cggatttata acgggaaaga gacaataaca ctgaagccag cgtccacgga ttatgtctat
      961 aaaactgagt ctgcaagcaa agataatggc tacgttttgt caacatatat gaaaccggga
     1021 tactggtcga gaacctcttc aggatggaaa ccggtcagcc gggagggcag aaatgatgtg
     1081 gcttactgtg agtttgtcac taaatatgcg aaatctttta ttcctggtgt acagcagatg
     1141 ccagcacaac tctatcagtc tccaacgggg catgagcttg aaatcattcc gttatccgat
     1201 ataagtcgtt tcagtgaaga tgtgaagctg caagtcctgt ataaaacgtc cccgctcgcc
     1261 ggagctatca tggagcttga ctcggtcagt tatctgaaat catcccgtca tactcatgca
     1321 gttgagcaca aacatcctgt tcataaagca gaactcacct ttgtaactaa tgaggatggt
     1381 atcgtcacag taccttctct tcatattgga cagtggctgg cgaaagtcca aaataagaaa
     1441 agttttcagg acaaaagcct gtgtgatgaa actgtcgatg tggcaacttt aagtttctcc
     1501 cgaaattaac aatactattc cctgccctga a
//
LOCUS       DAPHCV010000328         1504 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.333, whole genome shotgun sequence.
ACCESSION   DAPHCV010000328 DAPHCV010000000
VERSION     DAPHCV010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.333"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            50..1456
                     /gene="gndA"
                     /locus_tag="RH033_004931"
     CDS             50..1456
                     /gene="gndA"
                     /locus_tag="RH033_004931"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDV2993971.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILIKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT          373 a          358 c          391 g          382 t
ORIGIN      
        1 tgtatctttc caactcgaat tcttcgagag tccctgacag gagtaaacaa tgtcaaagca
       61 acagatcggc gtagtcggta tggcagtgat ggggcgcaac cttgcgctca acatcgaaag
      121 ccgtggttat accgtctcta ttttcaaccg ttcccgtgaa aagacggaag aagtgattgc
      181 cgaaaatcca ggcaaaaaac tggttcctta ctatactgtg aaagagtttg ttgaatctct
      241 ggaaacgcct cgtcgcatcc tgttaatggt gaaagcaggt gcaggcacgg atgctgctat
      301 tgattccctc aagccatacc tcgataaagg tgacatcatc attgatggtg gtaatacctt
      361 cttccaggac accattcgtc gtaaccgtga gctttctgcc gaaggcttta acttcattgg
      421 taccggtgtc tccggtggtg aagaaggcgc gctgaaaggt ccttccatta tgcctggtgg
      481 gcagaaagaa gcctatgaac tggttgcgcc gatcctgatc aaaatcgctg cagtggctga
      541 agacggtgag ccatgtgtta cctatattgg tgccgatggt gctggtcact atgtgaagat
      601 ggttcacaac ggtattgaat acggcgatat gcagctgatt gctgaagcct attcactgct
      661 taaaggtggc ctgaacctca ccaacgaaga actggcgcag acctttaccg agtggaataa
      721 cggtgaactt agcagctacc tgatcgacat caccaaagac atctttacta aaaaagatga
      781 agacggtaat tacctggttg atgtgattct ggatgaggcg gctaacaaag gtaccggtaa
      841 atggaccagc cagagcgcgc tggatctcgg cgaaccgctg tcgctgatta ccgagtctgt
      901 gtttgcacgt tatatctcgt ctctgaaaga tcagcgtgtt gccgcgtcta aagttctctc
      961 tggtccgcaa gcgcagccag caggcgataa agctgagttt atcgagaaag ttcgtcgtgc
     1021 gctgtatctg ggcaaaatcg tttcttatgc tcagggcttc tctcagctgc gtgcggcgtc
     1081 tgaagagtat aactgggatc tgaactacgg cgaaattgcg aagattttcc gtgctggttg
     1141 catcatccgt gcgcagttcc tgcagaaaat caccgatgct tatgccgaaa atccgcagat
     1201 cgctaacctg ctgctggctc cgtacttcaa gcaaattgcc gatgactatc agcaggcgct
     1261 gcgcgatgtc gtcgcttatg cagtacagaa cggtatcccg gttccgacct tcgccgctgc
     1321 ggttgcctat tatgacagct accgcgccgc agttctgcct gcgaacctga ttcaggcaca
     1381 gcgtgactat ttcggtgcgc atacttataa gcgcattgat aaagaaggtg tgttccatac
     1441 tgaatggctg gattaatctg atttaaccaa caataaattg aggcccggcg tatattgcgc
     1501 cggg
//
LOCUS       DAPHCV010000329         1503 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.334, whole genome shotgun sequence.
ACCESSION   DAPHCV010000329 DAPHCV010000000
VERSION     DAPHCV010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.334"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..279)
                     /locus_tag="RH033_004932"
     CDS             complement(<1..279)
                     /locus_tag="RH033_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HDV2993972.1"
                     /translation="MSDTHPGTTHPDNNNDRPWWGLPCTVTPCFGARLVQEGNQLHYL
                     ADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLY"
     gene            complement(359..580)
                     /locus_tag="RH033_004933"
     CDS             complement(359..580)
                     /locus_tag="RH033_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HDV2993973.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSTDTYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(643..1119)
                     /locus_tag="RH033_004934"
     CDS             complement(643..1119)
                     /locus_tag="RH033_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HDV2993974.1"
                     /translation="MQQLSFLPGAMTPGERSLIQRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(1135..>1503)
                     /locus_tag="RH033_004935"
     CDS             complement(1135..>1503)
                     /locus_tag="RH033_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDV2993975.1"
                     /translation="WPQHFGLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGA
                     FMAPEPDDDDDETWVLFNVMNGNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYY
                     RLRDYALQHPECSAIMRIID"
BASE COUNT          337 a          403 c          436 g          327 t
ORIGIN      
        1 atacagcgtg acggtatgct gatggcgggg attcagttca ccgctggtga gcatgagttc
       61 cagttgtttc atcagcagcg gaaaggcctg gtccagatga tacgcatccg cgtcgctaaa
      121 ctgccccctg ataccggcgc ggtcggcaag gtaatgcaac tgattgccct cctgcaccag
      181 acgtgccccg aaacagggcg tcacggtgca ggggagcccc caccaggggc ggtcgttgtt
      241 gttgtcggga tgcgttgtcc cggggtgtgt gtctgacacg ataaaatcct cacagaaaat
      301 cggttaagaa tgctccgatg gtggcgataa ttcggctcat cagaatgccc tttctgattc
      361 agttcagggt aacgctcatc agacggacat acggaccaaa actgtcctta cggcgttcag
      421 caaacacggc cagcacaccg ggaatatcct gcacttcacg gccggtatac gtatccgtgc
      481 taccgtgcca gcggtattta ccggtacaga acggaaaaag acgggatgca ggatgctgtt
      541 ggtgaatacg catggcttca ccacgggtga tgattttcat aatgggatac ctctgaagac
      601 agaagataaa agtgaaaaca ggtgtgatgt ggttgtgacg ggttaaagca gaccgtgttc
      661 ggcaaaggag aatacctggt tgccaccgac tatcagatgg tccggcaccc ggatatccac
      721 cagggccagc gcctgtacca gacgttcagt gataagccgg tctgccttac tgggggtgac
      781 ttcaccggac gggtgattgt gcgccagcac cacggcagcg gcattgtggt acagggcacg
      841 tttaatcacc tcccggggat ggacttctgt gcggttgatg gtgcctgtga agagggtttc
      901 acccgcaatc agctgattct ggttgttcag atacagcacc cggaactctt cccgctccag
      961 tcctgccatg ttcagaatca gccattcccg tgccgcatgc gtggaggtga aggccacgcc
     1021 gggctcatga agatgacggt ccagggtttt cagggcccgc tgaatgaggc tgcgctcgcc
     1081 gggcgtcatc gctccgggca gaaaggaaag ctgttgcatt atgcttctct ccattcagtc
     1141 gatgatgcgc ataatggcgc tgcattccgg atgctgcagg gcgtaatccc gcaaccgata
     1201 ataatggacc gtcatggcat aattctccgt acgacaggcg tgatgactgt acgtcatcag
     1261 acaggcggca atgccggcgg cttccgggct catttcagcg cggttaccgt tcatgacgtt
     1321 aaacagcacc catgtttcgt catcatcgtc atccggttcg ggtgccataa atgcgccacc
     1381 gttgttcagg gtgtacagat tccagatacc accgcagtag tcttcacaca gacggtccat
     1441 ccagccgaag acacggggct ccagggtcac ccactgtgga atgaggccaa aatgctgcgg
     1501 cca
//
LOCUS       DAPHCV010000330         1479 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.335, whole genome shotgun sequence.
ACCESSION   DAPHCV010000330 DAPHCV010000000
VERSION     DAPHCV010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.335"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..182)
                     /locus_tag="RH033_004936"
     CDS             complement(<1..182)
                     /locus_tag="RH033_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HDV2993976.1"
                     /translation="MLRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSL"
     gene            complement(169..594)
                     /gene="trbF"
                     /locus_tag="RH033_004937"
     CDS             complement(169..594)
                     /gene="trbF"
                     /locus_tag="RH033_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HDV2993977.1"
                     /translation="MNHRKYQRKLIMKEKRNDAELKNRKTKRDYDYERRVSDIYFDLF
                     FVFVAAGTFLWVIMHSIFDACIDSWKADPELNNFRYMWNILMYVIPYTLWAFAGGFLI
                     VYVRNPLNELINGGIRIFRLKRRMRREKKLREGGNNASH"
     gene            complement(542..889)
                     /gene="trbJ"
                     /locus_tag="RH033_004938"
     CDS             complement(542..889)
                     /gene="trbJ"
                     /locus_tag="RH033_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HDV2993978.1"
                     /translation="MPPVLWRGWIPFCRCTERKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRMNGLNESS
                     EISEETYNERKKK"
     gene            complement(819..1364)
                     /gene="trbB"
                     /locus_tag="RH033_004939"
     CDS             complement(819..1364)
                     /gene="trbB"
                     /locus_tag="RH033_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HDV2993979.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAVQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     ASFMARMDTVLQMYGEEKGTK"
     gene            complement(1351..>1479)
                     /locus_tag="RH033_004940"
     CDS             complement(1351..>1479)
                     /locus_tag="RH033_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone family protein"
                     /protein_id="HDV2993980.1"
                     /translation="LFGAYQVLDAWIARVSLEEREALEARQQAMMEGQQEGGHVSH"
BASE COUNT          387 a          402 c          334 g          356 t
ORIGIN      
        1 agggagccac cataggcata cccggcggcc tgcccctgcc agacgcccgg ttgtgtggta
       61 ttggaagcaa atcccagctt attaaagaac tgattcatgt cgctgttcac atcagctgac
      121 gctgcgggtg tgaccgtcag taaagcggca caaagaacca gaagaggttt aatgcgaagc
      181 attattgcca ccctccctta atttcttctc ccggcgcata cggcgtttca gccggaatat
      241 tctgatcccc ccgtttatta attcatttaa agggttcctg acgtatacga taagaaatcc
      301 acctgcaaac gcccataacg tatacggaat gacatacatc aggatgttcc acatataacg
      361 aaaattattt aattccgggt cagctttcca cgaatcaata caggcatcaa aaatactatg
      421 cataatcacc cataaaaaag ttccggcagc aacgaacaca aaaaacagat caaaatatat
      481 atcagaaacc ctgcgttcat aatcataatc ccgtttagtt tttctgtttt ttagttccgc
      541 atcatttctt ttttctttca ttataagttt cctctgatat ttccgatgat tcattcagac
      601 cgttcatgcg gctgtaatat atttcagaca ccgaaatcca tcccacaatt gcccccacaa
      661 aaaacacaac agaaacagaa aaggtaagac acagcgaaac gacaatttcc acagggctgg
      721 cgggataaaa aagcccctga cggataaagg ggatattcag gctgacgatt attcccgtcg
      781 cgatgccgga tattccggca atgagtaaca ccacctgctt atttcgtacc tttttcctct
      841 ccgtacatct gcagaacggt atccatccgc gccataaaac tggcggcatc cgttgccccc
      901 tgcaaaagcg gtaatgcctc aagtgtgttg acgttgacca ggaaggtagt cggtgtggcc
      961 accgggatat tcgggaagaa ggtctgcatc acgtccggtg gcaccggtaa tgcttccgga
     1021 aaggcggtat ccccctgacc atccagggtg tagggaaaaa cggaaaagcc gtactgctgt
     1081 gccagctgtt tcagtaccgg gtcaaactgg tgacagtaag ggcaatgccc ctgcataaac
     1141 agcaccacct tccagtcggc caggttaacc tgcctgccgt tgctgaggcg gaaccagcgg
     1201 ggagccggct tttctgttct ggctgatgtg tctgctgccg gttgtgcagg ctgcacggcc
     1261 ataccctgcg gattccagag tcgttcaatt tcatcccggg tggaggcctg cacacctgtg
     1321 gccatcatca acaaaatgac cggcagtaat ttagtgagag acatgtccgc cctcctgctg
     1381 cccttccatc atggcctgtt gcctggcttc cagtgcctca cgctcttcca ggctgacacg
     1441 tgcaatccat gcatccagca cctggtatgc gccgaacag
//
LOCUS       DAPHCV010000331         1450 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.336, whole genome shotgun sequence.
ACCESSION   DAPHCV010000331 DAPHCV010000000
VERSION     DAPHCV010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.336"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            8..1189
                     /locus_tag="RH033_004941"
     CDS             8..1189
                     /locus_tag="RH033_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV2993981.1"
                     /translation="MEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTNLSLS
                     VWAALLMLGSMLFLPSSPWWGKQISLTGSKTVVLWALGGYGVSFTLLGLGSVLMATGA
                     VTTAVGLGILIIARIVYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSCGRLF
                     GPLCAAAMLVIHPLAPVWMLMAAPVWAVVMLLRLPGTPPQPTPERKSVSLKRDCLPYL
                     LCAMLLAAAMSMMQLGLSPALTRQFDTDTTTISQQVAWLLGLSAIAALIAQFVVLRPQ
                     RLTPVALLLSAGVLMSSGLAIMLTEQLWLFYLGCAVLSFGAALATPAYQLLLNDKLAD
                     GAGAGWLAASHTLGYGLCALLVPLASKTGVSIALIVTALFAAVLFTIVSACIWHYRTI
                     K"
BASE COUNT          284 a          371 c          424 g          371 t
ORIGIN      
        1 atctaagatc gaagatacgc cccaaaaaac cctgtcctgc tggccactgg cgttcagtgc
       61 cggtcttctc ggtatcggac agaacggtct gctggttgta ctccctgttc tggtcataca
      121 gacaaatctg agtctgtctg tatgggctgc cctgctgatg ctgggctcaa tgctgtttct
      181 gccatcttcc ccatggtggg gaaagcaaat ttcccttact ggcagtaaga ctgtggtgct
      241 gtgggctctg ggaggatatg gcgtaagctt taccctgctt gggctgggaa gcgtgctgat
      301 ggctaccggt gccgtaacaa cagcggtggg gttgggaata ttaatcatcg cccggatcgt
      361 ctacggtctg accgtgtcag caatggtgcc agcctgtcag gtctgggcat tgcagagagc
      421 gggagaaggg aatcgcatgg ccgctctggc aaccatcagc tccggcctga gctgcggcag
      481 gctattcggg ccgctgtgcg cggcggcaat gttggtcatt caccctctgg cgccagtgtg
      541 gatgctgatg gcagctccgg tgtgggctgt ggtgatgctt ctgcggttgc ccggcacacc
      601 accacagccc acaccggagc gcaagagcgt cagcctgaag cgggattgcc tgccttatct
      661 gctttgcgca atgttactgg ctgcggcaat gagcatgatg cagcttggac tttcgccagc
      721 ccttactcgc cagttcgata ctgataccac cactattagc caacaggtgg cgtggttgtt
      781 ggggctgtcc gcaatagctg cgcttatcgc gcagttcgtg gtactccgtc cacagcgcct
      841 gactccagtg gctctgctcc tgagtgccgg ggtgttgatg agtagtggtc tggctatcat
      901 gctcactgaa cagctatggt tgttttacct gggctgtgca gtgctgtcat ttggggccgc
      961 tctggcaaca cccgcttatc aacttttact gaatgataag ctggccgacg gcgcaggcgc
     1021 gggctggctt gcggccagtc acacacttgg ctatgggctg tgcgcattgt tggtgccact
     1081 ggcgtcgaaa acaggtgtct caatcgcgct gattgtgacg gcattatttg ccgctgtatt
     1141 atttaccata gtgtctgcat gtatttggca ttaccgtaca atcaagtaag gctatgatgt
     1201 tgtactcaca gctggcataa cgtcctgtgc aatttcgctg tcgaaaccgc ctgtgctgac
     1261 cgcccagtgc actcacctga agcacatcgc agcatgccac caggatgcca gatcgtcttc
     1321 tgaaatgggt ggatacattg gcaacgttaa ccgcaacaat ttttcagaga gaattttggg
     1381 gggtgagggc cagtgatgta aaaaatacgc caagaggtta attcattgtt tatactgata
     1441 tgtcaggtaa
//
LOCUS       DAPHCV010000332         1440 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.337, whole genome shotgun sequence.
ACCESSION   DAPHCV010000332 DAPHCV010000000
VERSION     DAPHCV010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.337"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..618
                     /locus_tag="RH033_004942"
     CDS             <1..618
                     /locus_tag="RH033_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HDV2993982.1"
                     /translation="VNVSFKTFTRGEIAHLVIDSTGLKVFGEGEWKVKKHGKERRRIW
                     RKLHLAVDSNTHEIICADLSLNNVTDSEAFPGLIRQTHRKIRAASADGAYDTRLCHDE
                     LRRKKISALIPPRKGAGYWPGEYADRNRAVANQRMTGSNAQWKWTTDYNRRSIAETAM
                     YRVKQLFGGSLTLRDYDGQVAEAMALVRALNKMTKAGMPESVRIA"
     gene            complement(1083..1292)
                     /locus_tag="RH033_004943"
     CDS             complement(1083..1292)
                     /locus_tag="RH033_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FaeA/PapI family transcriptional regulator"
                     /protein_id="HDV2993983.1"
                     /translation="MKKKLILNFIQEINAPCQTTEIAIRFDMSAYQARHYLMCLEKEG
                     EIRRTPLRRGVRTLWEAARETEKHQ"
BASE COUNT          424 a          325 c          337 g          354 t
ORIGIN      
        1 gttaatgtca gtttcaaaac gttcacccgg ggtgaaatcg cgcatctggt gattgattcc
       61 accgggctga aggtctttgg tgaaggcgaa tggaaagtca aaaaacacgg caaagaacgc
      121 cgtcgtatat ggcgaaagtt gcatctggca gttgacagca acacacatga aatcatctgt
      181 gcagacctgt cgctgaacaa tgtgacggac tcagaagcct tcccgggtct tatccggcag
      241 actcacagaa aaatcagggc agcatcggca gacggcgctt acgacacccg gctctgtcac
      301 gatgagctgc ggcgtaagaa aatcagcgcg cttatccctc cccgaaaagg tgcgggttac
      361 tggcccggtg aatatgcaga ccgtaaccgt gcagtggcta atcagcgaat gaccgggagt
      421 aatgcgcagt ggaaatggac aacagattac aaccgtcgct cgatagcgga aacggcgatg
      481 taccgggtaa aacagctgtt cgggggttca ctgacgctgc gtgactacga tggtcaggtt
      541 gcggaggcta tggccctggt acgagcgctg aacaaaatga cgaaagcagg tatgcctgaa
      601 agcgtgcgta ttgcctgaaa aaacaacccg ctacggggga gacttacccg aaatctgatt
      661 tattcaacaa agcctcgccg gagcaaaata caaacagctc attgatatta acaaaaaaac
      721 tcagatggaa aaccactccg tggaatatgg gatgaccagc ctccatttat taatacccag
      781 tgatgttagt aatatcttta tcagcaacct ccggatatcc ctgtaaagaa gcattttccg
      841 gggaataact cacccgtatt cttcacggga ccgcaccatg gggggggagg caggtttcgg
      901 cagattaaca ccccatgata aagtagtgca aataaagcgg tggaagtagc gatacagtcc
      961 gcgtcacaga aacagataat gccgttgagg gactggtgat aagccgcctt actgttgagt
     1021 ttagtgagtg ccttgatgtt cacctttaag aaaagatatt tacacaaaat ggtaaacagt
     1081 tgttattgat gcttttccgt ttctctggct gcctcccata aagtacggac accacgacga
     1141 agaggcgtac gcctgatttc tccctctttc tcaagacaca taagataatg gcgagcctga
     1201 taagcactca tatcaaaacg tatagcgatt tctgttgttt ggcatggtgc gtttatctcc
     1261 tgaataaaat tcagaatcaa ctttttcttc attcctgttc aatccgtatc tgtatatcaa
     1321 ttttattata acctgtcttt cgctgcagaa atctcttttg tttcatatca aagaaataaa
     1381 aaaacaaaca aagcaacaaa caccttacaa tatttctatt aatttgttac tttttttgag
//
LOCUS       DAPHCV010000333         1425 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.338, whole genome shotgun sequence.
ACCESSION   DAPHCV010000333 DAPHCV010000000
VERSION     DAPHCV010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.338"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            97..>1425
                     /locus_tag="RH033_004944"
     CDS             97..>1425
                     /locus_tag="RH033_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDV2993984.1"
                     /translation="MNINRIIFAIEDCISTLSRYSVSAFFPDYCDLHTVIGLDQQDRE
                     RRPWLKAPYIATTKQGYYLSVFEISGALKEMDENPDLTAPGTLESLITALAERMNTAY
                     KNTGHKISFVFERDPERGRDEIEAMLLPQRKSIARTGILLQDVLDEKVTTLTPWLVRE
                     RCWLTVWSSEELLSRTDRKDHQTRIRKLAEHAPPARFAQDPWRWTLSALKIRHDAFLD
                     TLEQALTHDSDGLLVRLMDIHEVGREIRRQLERNSTPAVWQPHLPEDARPAGWRQGED
                     TSVFHAPSLNLQLFTTQPETHGSLIKAGDLWHGMVAITLPPQNLRTFNQLVRDVPHAI
                     PWRIRMDLMPGGMKALGVKKTLLTYSSFISPLRPMYDSVMMLNDINKHDPVCIMTIVA
                     TTWGNSREVCTRNQALLKSALEGWGVCGTTTTFGDPRRAWVNTVLAASHGS"
BASE COUNT          335 a          435 c          357 g          298 t
ORIGIN      
        1 agcaatactc actctgcaca acttacacgc atagcgttcc catttgaata cagttgcagc
       61 ccgcgattta ttttgtcttc cggagccacc tctgccatga atatcaaccg cattattttt
      121 gccattgaag actgtatcag taccctgtca cggtattccg tttccgcatt cttccccgat
      181 tactgtgatc tgcacaccgt catcggtctg gaccagcagg accgcgaacg ccgcccctgg
      241 ctgaaagccc cctacattgc caccacaaaa caggggtatt acctttccgt ctttgaaatc
      301 agcggtgccc tgaaagaaat ggatgaaaac ccggacctga cagcccccgg cacactggaa
      361 tcactgatta cagcgctggc agagcgaatg aacactgcct ataaaaacac cggtcacaaa
      421 atcagttttg ttttcgaacg cgatccggag cgtggaaggg atgaaatcga agccatgctc
      481 ttaccccagc gaaaatccat cgcacgcacg gggatcctgc ttcaggacgt tctggatgaa
      541 aaagtcacca cgctcacccc ctggcttgtc cgggaacgct gctggctgac cgtctggagc
      601 tctgaagaac tgctgagccg gaccgatcgc aaggaccacc agacccgtat caggaaactc
      661 gctgaacacg ctccgccagc ccgatttgcg caggatccat ggcgctggac actttccgca
      721 ctcaaaatac ggcatgacgc ctttctggac acgctggagc aggccctgac acatgattct
      781 gatggtctgc tggttcgcct gatggatatt catgaagtgg gacgggaaat ccgccgtcaa
      841 ctggagcgca acagcacacc tgcagtatgg cagcctcatc tgcctgaaga tgcccgtcct
      901 gccggctggc gacagggcga ggatacctct gtattccacg caccgtcgct gaatctgcag
      961 ttattcacca cacagcctga aacacacggc agcctgataa aggccggtga cctctggcac
     1021 ggtatggtcg ccattaccct gccaccccag aatctgcgga cattcaacca gctggtcagg
     1081 gatgtgcccc acgcaatccc ctggcgtatc cggatggatc tgatgcccgg cggtatgaag
     1141 gcgcttgggg tcaagaaaac cctgctgacc tacagcagct ttatttcccc tctccggccc
     1201 atgtatgact cggtgatgat gctgaatgac atcaacaaac atgatccggt ctgtatcatg
     1261 accattgttg ccaccacatg gggaaacagc cgggaagtct gtacccgcaa ccaggcgctc
     1321 ctgaaatctg cgctggaagg ctggggagta tgcggcacca ccaccacctt cggtgatccg
     1381 agacgagcct gggtgaacac ggttctggcg gcctcccacg gttct
//
LOCUS       DAPHCV010000334         1401 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.339, whole genome shotgun sequence.
ACCESSION   DAPHCV010000334 DAPHCV010000000
VERSION     DAPHCV010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.339"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            93..347
                     /gene="repA"
                     /locus_tag="RH033_004945"
     CDS             93..347
                     /gene="repA"
                     /locus_tag="RH033_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HDV2993985.1"
                     /translation="MSQTENAVTSSSGAKRAYRKGNPLSDAEKQRLSVARKRASFKEV
                     KVFLEPKYKAMLMQMCHEDGLTQAEVLTALIKSEAQKRCM"
     gene            585..659
                     /gene="tap"
                     /locus_tag="RH033_004946"
     CDS             585..659
                     /gene="tap"
                     /locus_tag="RH033_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HDV2993986.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            652..>1401
                     /gene="repA"
                     /locus_tag="RH033_004947"
     CDS             652..>1401
                     /gene="repA"
                     /locus_tag="RH033_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HDV2993987.1"
                     /translation="MTDLHQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVVWENKQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDADRERQDIVTLVKRQLTREIAEGRF"
BASE COUNT          355 a          339 c          382 g          325 t
ORIGIN      
        1 acagatcttc gtcacaattc tcaagtcgct gatttcaaaa aactgtagta tcctctgcga
       61 aacgatccct gtttgagtat tgaggaggcg agatgtcgca gacagaaaat gcagtgactt
      121 cctcatctgg cgcaaaacga gcatacagaa aggggaatcc gctttctgat gcagagaaac
      181 aaagattatc agtggcccgt aaaagagctt cgttcaagga agtaaaagta tttcttgaac
      241 caaagtataa ggccatgctc atgcaaatgt gtcatgaaga tggtctgact caggctgaag
      301 ttctgaccgc actgataaaa agtgaagcgc aaaaacgatg catgtgatga tgggcttaca
      361 ttcttgagtg ttcagaagat tagtgctaga ttactgatcg tttaaggaat tttgtggctg
      421 gccacgccgt aaggtggcaa ggaactggtt ctgatgtgga tttacaggag ccagaaaagc
      481 aaaaaccccg ataatcttct tcaactttgg cgagtacgaa aagattaccg gggcccactt
      541 aaaccgtata gccaacaatt cagctatgcg gggagtatag ttatatgccc ggaaaagttc
      601 aagacttctt tctgtgctcg ctccttctgc gcattgtaag tgcaggatgg tgtgactgat
      661 cttcaccaaa cgtattaccg ccaggtaaag aacccgaatc cggtgtttac accccgtgaa
      721 ggtgcaggaa cgctgaagtt ctgcgaaaaa ctgatggaaa aggcggtggg cttcacttcc
      781 cgttttgatt tcgccattca tgtggcgcat gcccgttcgc gaggtctgcg tcgacgcatg
      841 ccaccggtgc tgcgtcgacg ggctattgat gcgctcctgc aggggctgtg tttccactat
      901 gacccgctgg ccaaccgcgt ccagtgctcc atcaccacgc tggccattga gtgcggactg
      961 gcgacggagt ctgctgccgg aaaactctcc atcacccgtg ccacccgggc cctgacgttc
     1021 ctgtcagagc tgggactgat tacctaccag acggaatatg acccgcttat cgggtgctac
     1081 attccgaccg atatcacgtt cacatctgca ctgtttgctg ccctcgatgt atcagaggag
     1141 gcagtggccg ccgcgcgccg cagccgtgtg gtatgggaaa acaaacaacg caaaaagcag
     1201 gggctggata ccctgggcat ggatgaactg atagcgaaag cctggcgttt tgttcgtgag
     1261 cgttttcgca gttatcagac agagcttaag tcccggggaa taaagcgtgc ccgtgcgcgt
     1321 cgtgatgcgg acagggaacg tcaggatatt gtcaccctgg tgaaacggca gctgacgcgc
     1381 gaaatcgcgg aagggcgctt c
//
LOCUS       DAPHCV010000335         1389 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.340, whole genome shotgun sequence.
ACCESSION   DAPHCV010000335 DAPHCV010000000
VERSION     DAPHCV010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.340"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            120..1331
                     /gene="bluF"
                     /locus_tag="RH033_004948"
     CDS             120..1331
                     /gene="bluF"
                     /locus_tag="RH033_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HDV2993988.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQATYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFAMAHALELGDKMISINLLPMTLVNEPDAVSFLLDEIKANGLVPEQIIVEF
                     TESEVISRFDEFAEAVKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVTTPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSVAWPEKK"
BASE COUNT          393 a          290 c          303 g          403 t
ORIGIN      
        1 actttttcca ttttttatca atgtaaagaa acagttatgt tttggtatta aaagaaagac
       61 accggaagat atgtctgtta ccgtcttact ctcgcctctc ccattaccct ggacagttaa
      121 tgcttaccac ccttatttat cgtagccata tacgtgacga cgaacctgtc aaaaaaatcg
      181 aagaaatggt ttcgatagca aatcgcagga acatgcagtc tgacgtaaca gggatcttac
      241 tgtttaatgg atctcatttt ttccagcttc tggaaggtcc ggaagaacag gttaaaatga
      301 tatatcgggc tatatgccag gatccacggc actataatat tgttgagctg ctgtgcgatt
      361 acgcgcctgc tcgccgtttt ggcaaagcgg gaatggaatt atttgatttg cgcctgcacg
      421 agcgagatga cgttttacag gccgtattcg acaaaggcac atcaaaattt cagctaactt
      481 atgatgacag agcgctacaa ttttttcgta cttttgtcct tgcaaccgaa caagcaacct
      541 atttcgagat ccctgccgaa gactcctggc tttttatcgc tgacggatct gataaagaac
      601 ttgattcctg taccctttca ccaactataa acgaccactt tgcctttcat cctattgtcg
      661 atcccttatc gcggcggata atcgcttttg aagccattgt gcaaaaaaat gaagacagcc
      721 cgtcagctat agcggttggg cagcgtaaag acggggaaat ctacacagcg gatcttaaaa
      781 gtaaggcgct tgcattcgcg atggcacacg cacttgagct cggtgataaa atgatatcaa
      841 tcaatctatt acctatgacc ctggttaacg agcctgacgc agtctctttt ttacttgatg
      901 aaataaaggc caatggtctg gtgcctgaac aaatcatcgt tgaatttact gaaagtgaag
      961 tcatatctcg gtttgatgag tttgccgaag cggttaaatc gctaaaggct gctggtatca
     1021 gtgtagcaat tgatcatttt ggcgcaggtt ttgccggttt gttactcctg tcacgcttcc
     1081 agcctgacag aattaaaatc agtcaggaat tgattaccaa tgttcataaa agtgggccac
     1141 ggcaggcaat tattcaggcg atcataaaat gctgtacatc acttgaaatt caagtctctg
     1201 ctatgggtgt gacaacacca gaagagtgga tgtggcttga atctgcagga attgagatgt
     1261 ttcagggaga tctgtttgcc aaagctaaat tgaatggtat cccttcagtt gcgtggccgg
     1321 agaaaaaata attttcagca cattatttcg catgatttct ggaaatcatc gtgaaaatga
     1381 atacgttat
//
LOCUS       DAPHCV010000336         1376 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.341, whole genome shotgun sequence.
ACCESSION   DAPHCV010000336 DAPHCV010000000
VERSION     DAPHCV010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.341"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>1376
                     /locus_tag="RH033_004949"
     CDS             <1..>1376
                     /locus_tag="RH033_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDV2993989.1"
                     /translation="ARQEIGVLIGELARGNLGALRGSGITLANRAGWIDTLMSPKGMM
                     LGGVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAGQLWTLSRAISGNG
                     ITQHAAAGALAQVVGSGAFRGNDIGMVARAAAQMERSVGQSVSDTINQFKRLKDDPVN
                     AAKALDNELHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETGRRTADIDNNLNAL
                     GSTLKYLSDLWSSFWDAAMNIGREDSLDEQISALQEKVSRAKRLPWTASSSQVEYDQQ
                     RLNELQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNETEAARHQREIARIN
                     AMQYADQAVRDAAIQRENERYEKALASGKKKTREPRNDEATRLLLQYSQQQAQVEGQI
                     AAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKDELIQALTLLD
                     VKQQELQK"
BASE COUNT          364 a          304 c          434 g          274 t
ORIGIN      
        1 gcccgccagg agataggcgt tctgataggt gaactggctc gcggcaattt aggtgcgctg
       61 aggggatccg ggataacgct ggctaaccgt gccggatgga tagacacact gatgtcaccg
      121 aaaggcatga tgctgggcgg ggttattggc ggtattgccg cggccgtcta tggtctgggt
      181 aaagcctggt atgatggtca gaaggagggg gaagaattta accgccagtt gtcgctgacg
      241 gggcattatg ccggagtcac tgccgggcag ctgtggacgc tcagtcgtgc tatttccggg
      301 aatggtatta cgcaacatgc tgcagccggt gcgctggctc aggtggtggg gagtggtgca
      361 tttcgtggaa acgatatcgg tatggtggcg agagctgccg cacagatgga gcgatcggtt
      421 ggccagtcgg tcagcgatac cataaatcag tttaagcggc tgaaggatga tcctgtaaat
      481 gccgcgaagg ctctggacaa tgagctgcat tttcttactg ccactcagct tgagcagata
      541 cgcgtccttg gggaacaggg gcggtccagt gatgcggcac ggatagccat gtctgcactg
      601 gcagaggaaa ccggtcggcg tactgcggat attgataata acctcaatgc gctgggcagt
      661 acgctgaagt atctgtctga tttatggagt agtttctggg atgcggccat gaatattggt
      721 cgtgaagact cgctggatga acagatttcc gctttacagg agaaagtgtc gcgggcgaaa
      781 agactcccct ggacggcatc atcttctcag gttgagtacg atcagcagcg tcttaacgag
      841 cttcaggaga aaaaacgcca gaaggatttg caggatgcaa aagagcaggc agagcgcaat
      901 tatcaggagc aacagaaacg ccgtaatgct gaaaatgctg cactgaaccg gatgaatgaa
      961 acggaagcag cacgacatca gcgtgaaatt gcgcgtatta atgccatgca gtacgccgat
     1021 caggctgtca gggatgcggc gatacaacgt gaaaatgaac gttacgagaa agccctggca
     1081 tccggtaaga aaaaaacacg cgaaccccgt aatgatgagg ccacccggtt attgctgcag
     1141 tacagtcagc aacaggcaca ggtggaagga cagattgctg ctgccagaca gtcagcaggc
     1201 attgccacgg aaaggatgac agaagcgcat aaacagcttc tggctctgca gcagcgcatc
     1261 agcgacctgg acgggaaaaa actgacggca gatgaaaaga gtgtgctggc ccgtaaagat
     1321 gaactgattc aggcactgac gctgctggat gtaaaacagc aggagcttca gaaaca
//
LOCUS       DAPHCV010000337         1347 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.342, whole genome shotgun sequence.
ACCESSION   DAPHCV010000337 DAPHCV010000000
VERSION     DAPHCV010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.342"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..76
                     /locus_tag="RH033_004950"
     tRNA            1..76
                     /locus_tag="RH033_004950"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34..36,aa:Lys,seq:ttt)
     gene            107..232
                     /locus_tag="RH033_004951"
     ncRNA           107..232
                     /ncRNA_class="other"
                     /locus_tag="RH033_004951"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(343..1227)
                     /gene="xapR"
                     /locus_tag="RH033_004952"
     CDS             complement(343..1227)
                     /gene="xapR"
                     /locus_tag="RH033_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HDV2993990.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPA
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKERIPADLYIVY
                     ETQQVTPALVKLLAALAQ"
     gene            complement(1268..>1347)
                     /locus_tag="RH033_004953"
     CDS             complement(1268..>1347)
                     /locus_tag="RH033_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="XapX domain-containing protein"
                     /protein_id="HDV2993991.1"
                     /translation="PNVLPGILSIVFMYVGYLVVKYFMP"
BASE COUNT          352 a          369 c          307 g          319 t
ORIGIN      
        1 gggtcgttag ctcagttggt agagcagttg acttttaatc aattggtcgc aggttcgaat
       61 cctgcacgac ccaccaatgt aaaaaagcgc cctaaaggcg cttttttgct atctgcgata
      121 ctcaaagatt cgaacctgca gcaggttcga gttgagcgca gcgaaacaac ggagccgctc
      181 gcggcgacgg cccgaagggc gagcgaagcg agtcatcctg cacgacccac caatgtaaaa
      241 aagcgcccta aaggcgcttt tttgctatct gcaatttgcg aaattgcctg atgcgcttcg
      301 cttgtcagac caactatgtc cggcagttcc cgtgcctctc atctactgcg ccaacgccgc
      361 cagcagttta accagcgcgg gcgtcacctg ctgcgtttca taaacaatat ataagtctgc
      421 cgggatgcgt tctttgagcg gacggaatat gacaccgggc cagttcatct gcgcgtagct
      481 gtccgctatc aatgtgatac caatacccat actgaccatg gcgagtaccg tttgcggttc
      541 attaacttcg cgaataacaa ccggtgaaaa tcccacctgc tggcaaaccc gctgcaaaaa
      601 atcccagtca gtgtacacgg gcggcattgt aacgaaatac tcgtcacgta gcgcttccag
      661 cgggacggtg gaaaatgatg agagatgatg ctcttcaggc atcgccacca gaaacgccga
      721 ttcatgcaac cgtaagctgg taaaacctgc cggtggttct gtcgccattc gccagatccc
      781 ggcatcaagt tcgcggcgtt ccagcagggc catttgcatc gcgggcatct tttcgcgaaa
      841 cagaacttca acgttaggat tttctctaag gaatcgccgc ataaccgggc gcatccgtcc
      901 ccacattgcc gttcccacaa ccccgagttc aatccgccct gcttctcccc gacctatttg
      961 ttcaacccga gccaatacat tatttgcatt caccagcaat cgacgcgatt cttccatcaa
     1021 gattttgccc gcgtgtgtca gtacgacgct gcgcgaatgg cgaataaaaa gctgcgtgcc
     1081 gagttgattt tccagctctt taatatgaat gctgagcgga ggctgagaca tatttaaacg
     1141 cgctgctgcg cgaccaaaat gcaactcttc cgctacggca agaaaataac ggagcaactt
     1201 aagatctgtt ctgtatacgc gttccataat taagtgctcc atataataaa tacatcgtat
     1261 taaattatca tggcataaag tatttcacca ccagatatcc gacatacata aacacaatgg
     1321 ataatatacc cggtaataca tttggta
//
LOCUS       DAPHCV010000338         1304 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.343, whole genome shotgun sequence.
ACCESSION   DAPHCV010000338 DAPHCV010000000
VERSION     DAPHCV010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.343"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            150..1193
                     /locus_tag="RH033_004954"
                     /pseudo
     CDS             150..1193
                     /locus_tag="RH033_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          386 a          269 c          337 g          312 t
ORIGIN      
        1 gatagaaatc gtaaaaagct tcgttttcat aaaaatctct ttcaataata tttgtagctg
       61 gaccagcccc ttgaaacgcc agatagtgga tgtatcttaa aataagagac aggcttggat
      121 atatgtctga cactggttct aattttgaga tgactggaac tttgccgggg ctggagcttc
      181 gtaaacgtaa aactcctcag gaaaaaattg gcattattca gcagacaatg gagcctggca
      241 tggcggtgtc acatgtggcg cgtttgcgtt gtattcagcc ctgcctgttg tttaagtgga
      301 aaaacaatct ctggaaggaa gtctcactgc tgttgcttca ggagagggtg tcgttcctgc
      361 ctcagaactg cctgccgcta taaaacaaat aaaagagtta cagctcttgc tgggtaaaaa
      421 accatggaag tttagatcct caaagaagtg gtggagtatg cgcaatcgcg aaaatggata
      481 gcgcatgtgc ccttgttacc ttcggacaag gaatagcccc ggtaagccgt gttctgggcg
      541 atactcaggc ggcagtccct aaatgaagga ctaccgttag tgaatgcaaa gcgcgtttac
      601 cggataatga gcgaaaatat cctgttgtta catgacaaac catcacgact gcagcgggaa
      661 cataaaggcc gaatatcggt aaggaaagcg accagcgttg atgttctgat ggctttgagt
      721 tcggttgcga tgatggcgaa aaagtccggg ttacgttcgc cctggactgt tgcgatcgtg
      781 aagctatcga ctgggcatcc aatacgggag gttacaataa agcgacagtg caggatgtga
      841 tgttaggagc gatagaaaaa ctctttggcg ataaagtacc ggaacagtcc atccggtggt
      901 tgacagataa cggttcagcg tatagagcgc atgaaacacg gcagttcgcc agagagttgg
      961 atctggagcc ctgcacaacg gcaatcagca gctctcagag taatggaatg gcagaaaggt
     1021 tagtgaaaac gatgaaggaa gactacattg cgttcatacc gaaaccgaat gtaataacgg
     1081 cattgcataa ccttgcatag gcgttcgaga attacaatga gaaccacccg catagtgcgt
     1141 taggttatct ctcaccacgg gaatatcgat gtcagcggat aacgtcacct tagatgtagc
     1201 agttgccaaa cagtgactca agggcaggct taccgcatac gctgacactt agctgatcga
     1261 cagaatatta ttaacagttc atcactgaac gctacataat caga
//
LOCUS       DAPHCV010000339         1287 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.344, whole genome shotgun sequence.
ACCESSION   DAPHCV010000339 DAPHCV010000000
VERSION     DAPHCV010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.344"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            87..>1287
                     /locus_tag="RH033_004955"
     CDS             87..>1287
                     /locus_tag="RH033_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HDV2993992.1"
                     /translation="MCELDILHDSLYQFCPELHLKRLNSLTLACHALLDCKTLTLTEL
                     GRNLPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIR
                     EQKRLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVS
                     DAGFKVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKR
                     LTKSNPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWILATNLPVEIR
                     TPKQLVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWL
                     AGVHAQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDSLVAATLLTQNL
                     FTHGYVLG"
BASE COUNT          390 a          312 c          257 g          328 t
ORIGIN      
        1 tttatcaaaa tcattaagtt aaggtagata cacatcttgt catatgatca aatggtttcg
       61 cgaaaaatca ataatcagac aacaagatgt gcgaactcga tattttacac gactctcttt
      121 accaattctg ccccgaatta cacttaaaac gactcaacag cttaacgttg gcttgccacg
      181 ccttacttga ctgtaaaact ctcactctta ccgaacttgg ccgtaacctg ccaaccaaag
      241 cgagaacaaa acataacatc aaacgaatcg accgattgtt aggtaatcgt cacctccaca
      301 aagagcgact cgctgtatac cgttggcatg ctagctttat ctgttcgggc aatacgatgc
      361 ccattgtact tgttgactgg tctgatatcc gtgagcaaaa acggcttatg gtattgcgag
      421 cttcagtcgc actacacggt cgttctgtta ctctttatga gaaagcgttc ccgctttcag
      481 agcaatgttc aaagaaagct catgaccaat ttctagccga ccttgcgagc attctaccga
      541 gtaacaccac accgctcatt gtcagtgatg ctggctttaa agtgccatgg tataaatccg
      601 ttgagaagct gggttggtac tggttaagtc gagtaagagg aaaagtacaa tatgcagacc
      661 taggagcgga aaactggaaa cctatcagca acttacatga tatgtcatct agtcactcaa
      721 agactttagg ctataagagg ctgactaaaa gcaatccaat ctcatgccaa attctattgt
      781 ataaatctcg ctctaaaggc cgaaaaaatc agcgctcgac acggactcat tgtcaccacc
      841 cgtcacctaa aatctactca gcgtcggcaa aggagccatg gattctagca actaacttac
      901 ctgttgaaat tcgaacaccc aaacaacttg ttaatatcta ttcgaagcga atgcagattg
      961 aagaaacctt ccgagacttg aaaagtcctg cctacggact aggcctacgc catagccgaa
     1021 cgagcagctc agagcgtttt gatatcatgc tgctaatcgc cctgatgctt caactaacat
     1081 gttggcttgc gggcgttcat gctcagaaac aaggttggga caagcacttc caggctaaca
     1141 cagtcagaaa tcgaaacgta ctctcaacag ttcgcttagg catggaagtt ttgcggcatt
     1201 ctggctacac aataacaagg gaagactcac tcgtggctgc aaccctgctt actcaaaatc
     1261 tattcacaca tggttacgtt ttgggga
//
LOCUS       DAPHCV010000340         1250 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.345, whole genome shotgun sequence.
ACCESSION   DAPHCV010000340 DAPHCV010000000
VERSION     DAPHCV010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.345"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(128..670)
                     /locus_tag="RH033_004956"
     CDS             complement(128..670)
                     /locus_tag="RH033_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV2993993.1"
                     /translation="MKNLSLSVLLLLAGGGGSHAAVQKTVFSVDVVASACHVVVDADS
                     TGNSGRLTFGTYRKSTGASVPPRDFTVRLYESGATVQGCSAFKAGRVATLDFGNPGQL
                     DAGGVVTRGAGDGIRVDVRAVDAQADYRERLTQDSHSVNYPVEFAAKGQFRFRAQPVF
                     PANVKAGEYTGALTFVVTYQ"
     gene            complement(678..977)
                     /gene="daaA"
                     /locus_tag="RH033_004957"
                     /gene_synonym="afaA"
     CDS             complement(678..977)
                     /gene="daaA"
                     /locus_tag="RH033_004957"
                     /gene_synonym="afaA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F1845 fimbrial adhesin operon transcriptional
                     regulator DaaA"
                     /protein_id="HDV2993994.1"
                     /translation="MSSSNLYLSKKHGRTLSPGKMSESQFWLLAEISPVHSEKVLNAL
                     RDFLVLGYTRREACERHEVSQGYFSGALGRIQRTHQAVNRLVPFYISEAGIPYTG"
BASE COUNT          381 a          376 c          249 g          244 t
ORIGIN      
        1 cctgcaatac ctgtggcacc ggcaaaagca gctgatacgg caagcgcaag tgcggcccga
       61 cggaaaaaca cgaacgaaac cggctctgcg actgccttct ttcctgcaca cacaatcctt
      121 ttctccgtta ctgatacgtg accacaaagg tcagtgcacc cgtgtattcc cccgccttca
      181 cgttcgccgg aaacaccggc tgcgcacgaa aacggaactg tcccttagca gcaaactcca
      241 ccgggtaatt caccgaatga ctgtcctgag tcagacgttc gcgataatct gcctgcgcat
      301 ccaccgcccg cacatccacg cgaataccat caccggcacc gcgggtgacc acgcctcctg
      361 cgtccagctg gcccgggtta ccaaaatcca gggtggcgac ccgccctgcc ttaaaggctg
      421 agcagccctg aaccgtggca ccggactcat acagacgcac agtgaagtca cgcggaggca
      481 cagacgcccc cgtggacttg cggtaagtcc cgaatgtcag ccggccactg ttgccggtac
      541 tgtccgcatc caccaccaca tgacaggccg aggccaccac atcaacactg aatacggttt
      601 tctgaacagc ggcgtgagaa cccccgcccc ctgccagaag cagaagtact gaaagcgata
      661 aatttttcat gcaactgtta tcccgtatat ggaataccgg cttctgatat ataaaaagga
      721 accagcctgt ttaccgcctg atgggtacgc tgaatacgtc caagtgcacc agagaaatat
      781 ccctgagaaa cctcatgtcg ttcacaggcc tcccttcggg tataacccag aaccagaaaa
      841 tccctcaacg cattaagaac cttttcactg tgaaccggag atatttcagc taataaccag
      901 aactggctct ctgacatttt tccgggcgac aaagtacgcc cgtgcttctt gctcagatac
      961 aggttactgc tgctcattta tatccattcc agaaatataa ccagaccaaa aagacgacat
     1021 accccggata acaaaaacgt attcactctg ttgccgtcct gttttctgca gcgaagatta
     1081 tcacgatgtt ttatagcgaa aaacactaat ttaacgatct ttacaactac aaaaacaaac
     1141 atcaacaaca aataaaacca caaaaacaac aaacaacagc aaaagccacc aaaaaaacac
     1201 aaaaatcaca gataaaaaga tcgtctaaaa caaaaaaatc aaaattgcat
//
LOCUS       DAPHCV010000341         1247 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.346, whole genome shotgun sequence.
ACCESSION   DAPHCV010000341 DAPHCV010000000
VERSION     DAPHCV010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.346"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..696
                     /locus_tag="RH033_004958"
     CDS             <1..696
                     /locus_tag="RH033_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDV2993995.1"
                     /translation="PAVWFAYSPDRKGVHPQNHLAGYSGVLQADAYGGYRVLYESGRI
                     TEAACMAHARRKIHDVHARMPTDITTEALQRIGELYAIEAEVRGCTAEQRLAARKARA
                     APLMQSLYDWIQTQMKTLSRHSDTAKAFAYLLKQWDGLNVYCSNGWVEIDNNIAENAL
                     RGVAVGRKNWLFAGSDSGGEHAVVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPANRV
                     RDLLPWKVDLTSQ"
     gene            complement(728..1247)
                     /locus_tag="RH033_004959"
                     /pseudo
     CDS             complement(728..1247)
                     /locus_tag="RH033_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          281 a          340 c          350 g          276 t
ORIGIN      
        1 ccggcggtct ggttcgcgta cagcccggac cggaaaggcg tccatccaca gaatcatctg
       61 gccggttaca gcggtgtgct tcaggccgat gcttacggtg gttaccgggt gttatacgaa
      121 tccggcagaa taacggaagc cgcgtgtatg gctcatgccc ggagaaaaat ccacgatgtg
      181 catgcaagaa tgcccaccga catcaccacg gaagccctgc agcgtatcgg tgaactgtat
      241 gccatagagg cagaagtccg gggatgtaca gcagaacagc gtctggcggc aagaaaagcc
      301 agagctgcgc cactgatgca gtcactgtat gactggatac agactcagat gaaaacactg
      361 tcgcgtcact cggatacggc aaaagcgttc gcatacctgc tgaaacagtg ggatggcctg
      421 aacgtgtact gcagtaatgg ctgggtggaa atcgacaaca acatcgcaga gaacgcctta
      481 cggggagtgg ccgtaggccg gaaaaactgg ctcttcgcgg gttccgacag cggtggcgaa
      541 catgcggtgg tgttgtactc gctgatcggc acatgccgtc tgaacaatgt ggaaccagaa
      601 aaatggctgc gttacgtcat tgagcatatc caggactggc cggcaaaccg ggtacgcgat
      661 ctgttgccct ggaaagttga tctgacctct cagtaaatat caatacggtt ctggcgagcc
      721 gcttacattc atgcagcgac gtcagtaaca ctttgcttcg ctgctgcctg acggcaagac
      781 gctctgactc cggtaatccc cgtatttcat cctcgatggc gtacagttca ctgattcgct
      841 tcagggcttc ttctgccgtc gcacttttgc tgctgatgta tacatcgtgg atttttcgcc
      901 gggcatgggc ccagcacgca acttctgtca gtgcaccacc ttcacgttct gcactgaaca
      961 acctgtcgta acctgtgaac gcatccgcct gcaggatacc ccggaagggg cggaggtgtt
     1021 gctccgggtg tttcccctgc cggttcggcg agtacgcgaa ccagaccgct ggaggagatg
     1081 acgaacccac attgcgatca tcccggacat acgtccagat acgccctgtt ttcgcctttt
     1141 tctgacccgg tgccagtacc tttaccggtg tgtcatcagt gtgaaccttg cgggtgttca
     1201 ttacataacg gtacagggca tcattcaccg gtgtcattaa ctggcag
//
LOCUS       DAPHCV010000342         1231 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.347, whole genome shotgun sequence.
ACCESSION   DAPHCV010000342 DAPHCV010000000
VERSION     DAPHCV010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.347"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1161
                     /gene="pic"
                     /locus_tag="RH033_004960"
     CDS             <1..1161
                     /gene="pic"
                     /locus_tag="RH033_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HDV2993996.1"
                     /translation="KNADKINVTEKATGSDNSLNVSFMKDPAQGQSLNIPLVTAPAGT
                     SAEMFKAGTRMIGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYK
                     NFMTEVNNLNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELD
                     GVDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDY
                     TGNFAGLGTKHYNTHSWYAGAETGYRYHLTEETFIEPQAELVYGAVSGKTFRWKDGDM
                     DLSMKNRDFSPLIGRTGIELGKTFSGKDWSVTARAGTSWQFDLLNNGETVLRDASGEK
                     RIKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
BASE COUNT          338 a          256 c          357 g          280 t
ORIGIN      
        1 aaaaatgccg ataaaattaa tgtgactgaa aaagccacgg gttcagataa cagcctgaac
       61 gtcagcttta tgaaggatcc ggctcaggga cagtccctga atattcctct ggtcacggca
      121 ccggcgggaa cttcagcaga gatgtttaag gccggcaccc ggatgatagg tttcagtcgg
      181 gtgactccaa ccctgcatgt tgacaccagt ggtggcaata cgaagtggat actggacggt
      241 tttaaagcgg aggctgataa agccgctgcc gcgaaggctg acagtttcat gaatgccggg
      301 tataaaaact tcatgacgga agtcaacaat ctgaacaaac gtatgggtga cctgcgtgac
      361 acaaacggtg atgccggagc ctgggcgcgc atcatgagtg gtgccggttc tgcagacggt
      421 ggttacagtg ataattacac ccatgttcag gtcggctttg acaaaaaaca tgaactggac
      481 ggagtggacc tgtttaccgg tgtcacgatg acctataccg acagcagtgc agacagccat
      541 gcgttcagcg gcaagacgaa atcggtgggg ggcggtctgt atgcttcagc attgtttgag
      601 tccggtgcct atatcgattt gattggtaaa tatattcacc atgacaatga ttacacaggt
      661 aactttgccg gtctgggaac gaaacactac aacacccatt cctggtatgc cggtgctgaa
      721 acgggttacc gctatcacct gacagaggaa acgttcattg agccgcaggc tgaactggtt
      781 tacggcgccg tgtccgggaa aacattccgc tggaaagacg gtgatatgga cctgagcatg
      841 aagaacaggg acttcagtcc gctgattgga agaacaggga ttgaactggg caagaccttc
      901 agtggtaagg actggagtgt gacggcccgt gccgggacca gctggcagtt tgacctactg
      961 aataatggtg agacggtact gcgtgatgcg tccggggaga aacggataaa aggcgagaaa
     1021 gacagccgga tgctgtttaa tgttggtatg aatgcgcaga taaaggacaa tatgcgcttt
     1081 ggtctggagt ttgagaagtc agcctttggt aaatataacg tggataatgc ggtaaacgca
     1141 aatttccggt atatgttctg atgtggggga aaggctgccg gaaggcagcc tttctcagag
     1201 attcttaaaa tataaaacag tattttctta a
//
LOCUS       DAPHCV010000343         1224 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.348, whole genome shotgun sequence.
ACCESSION   DAPHCV010000343 DAPHCV010000000
VERSION     DAPHCV010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.348"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..395)
                     /gene="traK"
                     /locus_tag="RH033_004961"
     CDS             complement(<1..395)
                     /gene="traK"
                     /locus_tag="RH033_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HDV2993997.1"
                     /translation="MFGSLFFSCSVMAANGTLAPTVVPMVNGGQASIAISNTSPNLFT
                     VPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLSIQAVPREG
                     AGRTIQLVSDLRGTGEEAGAWETSTPYESL"
     gene            complement(406..972)
                     /gene="traE"
                     /locus_tag="RH033_004962"
     CDS             complement(406..972)
                     /gene="traE"
                     /locus_tag="RH033_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HDV2993998.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNVIQGVNNYRLQNE
                     QRTAVTPMAFNAPFAVSQNSADASYLQQMALSFIALRLNVSSETVDASHQALLQYIRP
                     GAQNQMKVILAEEAKRIKNDNVNSAFFQTSVRVWPQYGRVEIRGVLKTWIGDSKPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(994..>1224)
                     /gene="traL"
                     /locus_tag="RH033_004963"
     CDS             complement(994..>1224)
                     /gene="traL"
                     /locus_tag="RH033_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HDV2993999.1"
                     /translation="LDELIPAAICIGWGITTAKYLFGIGAAVLVYFGIKKLKKGRGSF
                     WLRDLIYWYMPTALLRGIFHNVPDSCFRQWIK"
BASE COUNT          319 a          312 c          297 g          296 t
ORIGIN      
        1 agggattcgt aaggcgtgga cgtttcccac gcaccggctt cttctccggt tccgcgcagg
       61 tcactgacca gctgaatggt acgccccgcg ccttcacggg gaacggcctg aatggaaaga
      121 ttcagaccac gttctgtttc cagaatgaac gtaaagggct ttttgttgac ggtggcaacc
      181 accacaccgc cggaggcggt ctgctcatta ttggtcaggg caccatccag actgttcacg
      241 gcgataatcc ggtcaccggg aacggtaaac agattcgggc tggtattgct gatggcaata
      301 ctggcctgac caccgttcac cattggcacc acggtggggg ccagcgtacc gtttgcggcc
      361 atcacgctgc aggaaaaaaa caggctgccg aatattattg ccgtattatt ttttctcatc
      421 gtctgtttcc ccgaaattat ccagccaggt caccccgttt tcccgcttca gaataaggat
      481 gtaatgtttg atatcagtga aaggttttga atcaccaatc caggttttaa gcacacctcg
      541 aatttccaca cggccatact gaggccagac acgaacactg gtctggaaaa aggctgagtt
      601 cacgttatcg tttttaatac gcttcgcttc ttcagccaga ataactttca tctggttctg
      661 tgcgcccggg cggatatatt gcagaagcgc ctgatgtgag gcatcgacgg tttctgatga
      721 aacattcaga cggagggcaa taaatgacag cgccatctgc tgtaaataag aggcgtcggc
      781 actgttctgt gacacggcaa agggggcatt aaatgccatt ggcgtcacgg cagtgcgttg
      841 ctcattctga agacggtagt tattcacccc ctgaatgacg ttaacagaga ggctgagaac
      901 aataagcact gacataaata taaaggcgat ggccattaca cgactggtac ttaaacgggc
      961 accgtgttcc atataacaat cctggtatca gttctattta atccactgcc ggaaacacga
     1021 atcaggaaca ttatgaaaaa taccgcgcag cagggctgtt ggcatatacc agtaaatcag
     1081 gtcacgtaac cagaaactgc cccgcccttt tttcagtttt ttaatcccga aataaaccag
     1141 aaccgctgca ccaataccga acagatattt cgctgttgtg ataccccagc caatacagat
     1201 tgctgcgggg atcagttcat ccag
//
LOCUS       DAPHCV010000344         1195 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.349, whole genome shotgun sequence.
ACCESSION   DAPHCV010000344 DAPHCV010000000
VERSION     DAPHCV010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.349"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..780
                     /locus_tag="RH033_004964"
     CDS             <1..780
                     /locus_tag="RH033_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HDV2994000.1"
                     /translation="LVQEAASLDSPLNAGEIRELRGRMADNERAISAPVTSVVPRISS
                     LTVNLSPGASLPLVRTALHNVSVVTFTDINGSPWPQSDPPYNAAPGHFDVQFNGNMVS
                     VTPLRAWAAGNVTVYLKGLSVPVILNITSGETDTNTLSQEIDSRLDLRIPRQGPASPV
                     VAVPSEKIALHDATLQAFLDGVPPDDPTVRRLKFSGNVPGTTIWQHGDDLLVRSRAML
                     RDEFEQTLASADGTRLWKLPVTPLLTFSVNGQSIHVTPELE"
     gene            784..>1195
                     /locus_tag="RH033_004965"
     CDS             784..>1195
                     /locus_tag="RH033_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HDV2994001.1"
                     /translation="MSAEQDTAAKSGKKLSALLVLGGVIIIGGGYLLLTWLSPENSAS
                     LSSVNINSTTTGSGRTVVESQHYRDLLRADNERGAAEALRNNQTFIASLPRGLDTDHG
                     NNQRRDTQPADQAEGSGQPSTPPQADQTAREKQRE"
BASE COUNT          270 a          363 c          326 g          236 t
ORIGIN      
        1 cttgttcaag aagccgcatc actggattcc cccctgaatg caggtgaaat tcgggaactg
       61 cggggacgaa tggccgataa cgaacgggcc atcagcgcac ctgtcaccag tgtggtgccc
      121 cgtatcagct ccctcacggt aaatctgtca cccggggcca gtctgccgct tgtccgaacc
      181 gcactgcata acgtcagcgt ggtaacgttc acggacatca acggctcccc gtggcctcag
      241 tcggatccgc cttataacgc ggcacccggt cactttgatg tgcagttcaa cggcaatatg
      301 gtctccgtca ctcccctgcg cgcctgggct gcagggaatg taacggtcta cctcaaagga
      361 ctgtctgtac cggtcattct gaatatcacc agtggtgaaa ccgacaccaa taccctgagt
      421 caggagatcg acagccgtct ggatctgcgc attccccgtc agggacctgc cagtcctgtg
      481 gtagccgtac catcagagaa gattgccctg catgatgcca ccctgcaggc ctttctggac
      541 ggtgttcctc ccgatgatcc caccgtgcgt cggctgaaat tctccggaaa tgttccgggc
      601 accaccatct ggcagcacgg cgatgatctg ctcgtgcgat cgcgggccat gctgcgggat
      661 gaatttgaac agacgctggc atctgctgac ggtacgcgtc tgtggaaact gccggtgact
      721 cccttactca ccttctccgt gaatggtcag tccattcacg tcacccctga actggagtaa
      781 atcatgagcg cagaacagga cacagccgca aagtccggca aaaaactgtc agccctgctg
      841 gttctcgggg gcgtgattat catcgggggc ggatacctgc tgctcacctg gctttccccc
      901 gaaaacagcg catcgctctc gtcagtcaat atcaacagca cgacaacagg cagcgggcgg
      961 accgtggtgg agtcacagca ctaccgggat ttactgcgtg ctgacaatga gcgcggggcc
     1021 gcagaagcgt tacgtaataa ccagacgttc atcgccagtc tgcccagggg actggatact
     1081 gatcacggaa ataatcaaag aagggatact cagcctgctg accaggccga gggttctggt
     1141 caaccatcaa cgccccctca ggcagatcaa acagcccggg aaaaacagcg ggaag
//
LOCUS       DAPHCV010000345         1178 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.350, whole genome shotgun sequence.
ACCESSION   DAPHCV010000345 DAPHCV010000000
VERSION     DAPHCV010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.350"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(129..338)
                     /locus_tag="RH033_004966"
     CDS             complement(129..338)
                     /locus_tag="RH033_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2994002.1"
                     /translation="MKTNAPRLFFVDKESGVSFDVFHVLSVSFPVTAVPHMTGECNLR
                     YLTENVPFAGTGGQSCMQDLYHSSG"
     gene            472..834
                     /locus_tag="RH033_004967"
     CDS             472..834
                     /locus_tag="RH033_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2994003.1"
                     /translation="MNDGRQTPPPPEAWKIRFLANGTPGTWFYTDDAAVTKEWLRAHC
                     DDVEVIPLCRMHDGPVFREHHINELSAALARMLHMHHILLERTRYRENIHDAECIREI
                     HEAPLQAIGILEKYKRTK"
     gene            847..1038
                     /locus_tag="RH033_004968"
     CDS             847..1038
                     /locus_tag="RH033_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2994004.1"
                     /translation="MWILIFWLSSPFNVSSGDARQPTLAVHSQAFRTEKACRDAFTAL
                     KKENVGEFALRGVCVPAGE"
BASE COUNT          310 a          337 c          284 g          247 t
ORIGIN      
        1 cgctcagtgc ctgccgccct gatatgtctg agagacattt ccgggatgtc agtcgactgg
       61 tttacttaac tgcgatgact aaagctggca gtaatgccgg cttttttacg gccacatacg
      121 tcttcctctc atccggaact gtgatacaaa tcctgcatac aggattgacc gccggttcct
      181 gcaaatggca cattttcagt aagatagcga aggttacatt cccccgtcat gtgtggcaca
      241 gctgtcaccg ggaaagaaac ggaaagcaca tgaaaaacat caaaactcac accggactcc
      301 ttatccacaa agaacaaacg cggcgcgttc gtcttcatga aacacccact gcctggtgcc
      361 acacgcaccg ggagtgctac agcaaaacga ccggtcgccg ctgcggcagc cctgacagtc
      421 tcagcaggct gattctgagc agcattcagc ccatagaatg agataacccc tatgaatgat
      481 ggcagacaga cgcctccgcc ccccgaggca tggaaaatcc ggtttctggc gaacggtaca
      541 cccggcacat ggttttacac cgatgacgca gccgtcacaa aagaatggct gcgggcacat
      601 tgtgacgatg tggaagtgat ccccctgtgc cgtatgcatg acggtcccgt tttccgggaa
      661 caccatataa atgaactcag cgccgcactg gccagaatgc tccatatgca ccacatactg
      721 ctggaacgaa cccggtacag agaaaatatc catgatgcag aatgtattcg tgaaatccac
      781 gaagcccccc tgcaggctat cggcatcctg gaaaaataca aacgcacaaa ataataaggc
      841 tgttctatgt ggatcctgat tttctggctc tcatccccct ttaatgtctc ctccggcgat
      901 gcccggcaac ccacactggc tgttcacagt caggcgttcc gtactgaaaa agcctgtcgg
      961 gatgccttca ccgcgctgaa aaaagaaaac gtgggggaat ttgctctccg tggcgtctgc
     1021 gtccccgccg gagaataaac aagacatgcg caaccggagt ccgctatgac atccccggac
     1081 cttatacaag gtgatctgca ccgaatgtcc tggtctcagc tggcaaaagc cgcggaagag
     1141 tcgacagtac atcatgatta tgcccgggcg ttaatatt
//
LOCUS       DAPHCV010000346         1178 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.351, whole genome shotgun sequence.
ACCESSION   DAPHCV010000346 DAPHCV010000000
VERSION     DAPHCV010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.351"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>1178
                     /locus_tag="RH033_004969"
     CDS             <1..>1178
                     /locus_tag="RH033_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
                     /protein_id="HDV2994005.1"
                     /translation="SYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNM
                     VMDASGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNSLSQFG
                     SNRGSSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASRSTRAS
                     AGMYGDAHAEWGVKPKILGVGGGLGVRGGGRAGIDWEDNDAHTASSNTQSSHNARHDI
                     DARATQDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTR
                     SHEYAEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRRAMA
                     WSFVQEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIDQRTQDSN
                     "
BASE COUNT          314 a          260 c          358 g          246 t
ORIGIN      
        1 tcctacggca acatgcagac ggaaaacgtg aacggcttca gctggagcac caacagcacc
       61 acgtcgtttg gtcagatgat gtaccagacc ggcagtggcg caactgccac acagacccgt
      121 gacggtaata tggtgatgga tgcgagcggg gcgatgtccc ggttaccggt cggtatcaat
      181 gcaacgcgtc agattgcagc ggcacaacag gaaatggccc gggaggcgtc gaacagagca
      241 gaaagcgccc tgcatgggtt cagcagcagt attgccagtg cctggaactc gctcagccag
      301 tttggttcta accgggggag cagtgattct gtcacaggtg gtgctgacag cacgatgagc
      361 gcacaggact ccatgatggc cagccgtatg cgcagtgcag tggaaagcta tgcgaaggcg
      421 cataatatca gtaatgagca ggcgactcag gaactggcat ccagaagtac gagagcttct
      481 gccgggatgt atggagatgc tcatgctgaa tggggggtta aacctaaaat tcttggtgtt
      541 ggtggaggat taggcgttag aggtggtggt cgggctggta ttgattggga agataatgat
      601 gcgcatacgg caagtagtaa tactcagtcg agccataatg ctcgtcatga tatcgatgcc
      661 agagctacac aagactttaa agaggccagc gattatttta ccagtcgcaa ggtcagtgag
      721 tccggcagcc atacggacaa taatgctgat tcccgtgtgg atcagttgtc tgcagccctg
      781 aattcagcga agcagagtta cgaccagtac acgacgaata tgacccgcag ccatgaatat
      841 gctgaaatgg catcccgtac tgaaagcatg agtgggcaga tgagtgaaga cctgtcgcaa
      901 cagtttgcac agtatgtaat gaaacacgcg ccacaggatg cagaagctat tctgacaaac
      961 accagttcac cggagattgc agaacgtcgt cgggcgatgg cctggtcttt tgtgcaggaa
     1021 caggtacagc ctggtgttga taatgcctgg cgggaatccc gtggtgatat tggtaaagga
     1081 atggagagcg taccttcggg tggtggcagt caggatatta ttgctgacca tcaggggcat
     1141 caggccatta ttgatcaaag aacgcaggac agtaatat
//
LOCUS       DAPHCV010000347         1169 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.352, whole genome shotgun sequence.
ACCESSION   DAPHCV010000347 DAPHCV010000000
VERSION     DAPHCV010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.352"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..1169
                     /locus_tag="RH033_004970"
                     /pseudo
     CDS             1..1169
                     /locus_tag="RH033_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc43 family transposase"
BASE COUNT          293 a          304 c          342 g          230 t
ORIGIN      
        1 catctgcggg ctcagataga aaaactgcgc cggatgctgt tcggaacccg ttctgaaaaa
       61 ctgcgtcgtg aggttgaaca ggctgaagcc ctgctgaaac aacgcgagca ggaaagcgat
      121 cgttacagtg ggcgtgagga tgacccgctg gttccccgcc agttgcgaca gtcgcgccat
      181 cgtcgtccgt taccggcaca tctcccccgt gaaatatacc gcctggagcc tgaagaaagc
      241 tgttgcccgg agtgtggcgg tgagcttgat tatctggggg aagtcagcgc agaacaactg
      301 gaactggtga gcagcgccct gaaagtgatc cgcacagaac gggtaaaaaa agcctgtaca
      361 aaatgtgact gcatcgttga agcaccggca ccatcccgtc cgatagagcg tggtatcgcg
      421 ggcccggggt tacttgcccg cgtgttaacg ggaaaatact gcgaacacct gccactgtat
      481 cgtcagagtg aaatctttgc ccgccagggt gtcgaactga gccgtgcctt actctccaac
      541 tgggttgatg cgtgctgcca gttaatgacg ccgctgaatg atgccctgta cagttatgtg
      601 atgaacaccc gcaaggttca cactgatgac acaccagtaa aagtactggc accgggcagg
      661 aagaaggcga aaacaggata tatctggacg tatgtccggg atgaccgaaa tgccggttcg
      721 ccagaacctc cggcggtctg gttcgcctac tcaccggacc atcagggtaa acatccggag
      781 caacaccttc gtcccttccg ggtatcctgc aggcagatgc gttcgcaggt tacgatcggc
      841 tgttcagtgc cgaacgtgaa ggcggcgcgt tgacggaagc aggatgctgg gctcatgcgc
      901 ggcgcaaaat ccacgatgta tatatcagta ccaaaagcgc gacggcggaa gaagcactga
      961 aactaatcgg cgaactgtac gccattgagc acgaaatacg cgggttgccg gtgtctgaac
     1021 gcctggcggt caggcaaatg cagagtaaac cgctactgac ttccctgtat aagctgatgc
     1081 aggagaaaga acacacgtta tcgaaaaaat gccgtctgag agatgcgttc cggtatatca
     1141 ggaagcactg ggttgcgttg tgcaacttc
//
LOCUS       DAPHCV010000348         1131 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.353, whole genome shotgun sequence.
ACCESSION   DAPHCV010000348 DAPHCV010000000
VERSION     DAPHCV010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.353"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(23..505)
                     /locus_tag="RH033_004971"
     CDS             complement(23..505)
                     /locus_tag="RH033_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2994006.1"
                     /translation="MRNKTREAMRLFLGGRCYTAEKLEKDYLAEVANYSNDRWEAPQR
                     AARLAASVKRYKTSEMLRFIFATIAYDPDPDLTPLTVRRLCKALFGRTGSQWLVVEVF
                     GEKGRQHRSADSNPEMVEKMAARYRHAAELHWSATLAEIERVKRLYQTKIKKSKKEVG
                     "
     gene            complement(622..>1131)
                     /locus_tag="RH033_004972"
     CDS             complement(622..>1131)
                     /locus_tag="RH033_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HDV2994007.1"
                     /translation="LDNTAEALEIGLTDAAGQVVFETRLKPTVAIGAQAAAVHGISEQ
                     ALCGAPSWTDMARQLRHAIGDRPVIIFNARFDIRILKQTAAAHSDPADWLEEMTVYCA
                     MELAAGYYGATNRYGTISLASAASQAGLTWEGLAHSAIVDARMAAGVVNAIAAYHLEL
                     LQEQARLKI"
BASE COUNT          246 a          327 c          274 g          284 t
ORIGIN      
        1 aggaaaagaa taacaatggc agtcaaccca cttctttttt ggatttctta attttcgtct
       61 gataaagccg ctttacccgt tcaatttctg ccagcgttgc ggaccagtgt agttcggcgg
      121 catgacgata tctggcagcc attttttcca ccatctccgg attgctgtcc gcgctgcggt
      181 gctgacgccc cttctcccca aaaacttcaa caaccagcca ctggcttccg gttctgccga
      241 acaatgcctt gcacaaacgt ctcacagtca gcggggttaa atctggatcg ggatcatagg
      301 caatggtagc aaaaataaag cgcagcattt ctgatgtctt gtaacgcttt acgctggcag
      361 ccaggcgtgc ggcacgttgc ggtgcttccc agcggtcgtt actgtaatta gccacttcag
      421 ccagataatc cttttcaagt ttttcagccg tgtagcacct gccgcctaaa aaaagccgca
      481 tggcctccct ggttttattt ctcacatatc atccctcatt aatgctaatg tacgcataca
      541 ttagcattaa catgctgaca gtaacaagcg gcggatttac gtctggataa cgtaaatgat
      601 tgcggtatac aggccaggcg gtcagatttt cagccgtgcc tgttcctgca gcagttccag
      661 atgatatgca gcaatagcgt ttaccacccc tgccgccatc cgtgcgtcga cgatcgctga
      721 gtgtgccagc ccttcccagg tcagtccggc ctggctggca gcactggcca gtgaaatcgt
      781 gccatagcgg ttggtggccc cataatatcc tgcagccagc tccatcgcac aatataccgt
      841 catttcttcc agccagtcag ccggatcgct atgtgcggcc gcagtctgtt tcagaatgcg
      901 gatgtcgaac cgggcattaa agataattac tggtcgatct ccgattgcat gccgcagttg
      961 ccgcgccata tctgtccatg atggtgcgcc gcaaagagcc tgctcactga taccatgcac
     1021 ggcggcggcc tgcgccccga tggcgaccgt gggcttcagc ctggtttcaa ataccacctg
     1081 accggccgca tcggtcaggc cgatttccag ggcttccgcg gtattatcca g
//
LOCUS       DAPHCV010000349         1129 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.354, whole genome shotgun sequence.
ACCESSION   DAPHCV010000349 DAPHCV010000000
VERSION     DAPHCV010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.354"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(260..451)
                     /locus_tag="RH033_004973"
     CDS             complement(260..451)
                     /locus_tag="RH033_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2994008.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(448..870)
                     /locus_tag="RH033_004974"
     CDS             complement(448..870)
                     /locus_tag="RH033_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HDV2994009.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFVYGACDRVVSDIVNELMEEKRASRHVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREELEAMDVVLQALDGETMS"
     gene            complement(917..>1129)
                     /locus_tag="RH033_004975"
     CDS             complement(917..>1129)
                     /locus_tag="RH033_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDV2994010.1"
                     /translation="MVNGANWFDRTVSADAAGIILTSLVINRQLWLYHDSGDAGLTHL
                     YRMRDAQLWSHIEFHPECNAIYAALD"
BASE COUNT          271 a          343 c          279 g          236 t
ORIGIN      
        1 ccagggggac gcccggaaaa gccataagca ggagaggaac ggaaagaaga ggaagaagag
       61 gaagaggaaa aagaagaaga acagtaacag gcacggtaac cacagaaaaa taacatcaga
      121 aaaaaacatt ccggacacat cacaaacagg aagggtaacg ggcgacgcgt cagcgtggcc
      181 cgcttccctt ccggcaggcg tcagcctgcc aggggttttc gcgcgggctg acaggcggcc
      241 tccaccgcct gcccctgtat cacctgtttc tgctgccctg aaggatgtag tgaccgccca
      301 ggaaaaccgc ctgctgattc gccacaaaat cgcagatttc atcaacaatc tgcgcggtgg
      361 tgagcggttt ctgccccatt tcacgacgcc gcgcattaat cacggccctg taattacgca
      421 gaatttcgcg ggtttctgtg ctgatattca tgacatcgtc tccccgtcca gcgcctgcaa
      481 cacaacgtcc attgcttcga gttcctccct gacgaatgcg tcaccgtcgc ccccgttctc
      541 actcccgacg gcatacaggc gcttcatttc actgcctgcc agcgccatca ctttttccag
      601 catcaccgcc gggactgtca catgacggct ggcgcgcttt tcttccatca gttcattgac
      661 aatatcggaa acaactctgt cacatgcgcc atatacaaaa gcatcgtcca gccgctgcat
      721 gacggtttcc agctcatcgt ctgacaggtt ccagtcctgg gctatgtgac gcacatcccc
      781 acgggtcagc accacggcaa aaacgcattc actgtccggc acgtctgtat ccagtacatc
      841 gcggatgatt tctttaacag tacagtacat gttctcgtct cctttacagg ggcggcattg
      901 ccgccccgtc cgttaatcag tcaagtgccg cgtaaatcgc gttgcattcg gggtgaaatt
      961 cgatgtggct ccacaactgc gcatcacgca tcctgtacag gtgtgtcaac cctgcatccc
     1021 cgctgtcgtg gtacagccac aactggcggt taatcacaag ggaggtaagg atgatgcctg
     1081 cggcatcagc actcacggta cggtcaaacc agtttgcacc gttcaccat
//
LOCUS       DAPHCV010000350         1127 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.355, whole genome shotgun sequence.
ACCESSION   DAPHCV010000350 DAPHCV010000000
VERSION     DAPHCV010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.355"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            20..358
                     /locus_tag="RH033_004976"
     CDS             20..358
                     /locus_tag="RH033_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type lysozyme inhibitor"
                     /protein_id="HDV2994011.1"
                     /translation="MKKYSSPVLALILVSSSMVSSAATKEISKTAYVCNGNKTMEVVY
                     INTTAGNSYAVINQMDEMIPMQLMKMASGANYEAINKRYSYKLYTKGKKANLVEGNDN
                     PVLSNCSSAE"
     gene            complement(740..>1127)
                     /locus_tag="RH033_004977"
     CDS             complement(740..>1127)
                     /locus_tag="RH033_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDV2994012.1"
                     /translation="VSSRIRYIEDAISDRRRVQSGRSFRYYFVFCDDEYTDIVRILFT
                     GEACCILNKSMLNLRAAGGVLTLEERRAVLNRYYRGLSIAEITEETGQNDKTVYGHLR
                     RALQRSGFRKGRFIKGREAAGDSGVE"
BASE COUNT          357 a          284 c          182 g          304 t
ORIGIN      
        1 acctacagaa ggagttatta tgaaaaaata ctcttctccc gtccttgcac tcattcttgt
       61 tagcagcagt atggtatcct ccgctgccac aaaagaaatc tcaaaaacag cttatgtctg
      121 taatggaaat aagacaatgg aggtggtcta tatcaacacc actgctggga actcatatgc
      181 agtgattaat cagatggatg agatgatacc tatgcagctt atgaaaatgg cctccggagc
      241 caattacgaa gccatcaata aaagatactc atataaactg tacacaaaag ggaaaaaagc
      301 caatctggtg gaagggaatg ataatcctgt cttaagcaac tgttcatcgg ccgaatgaca
      361 gccatcgtgc taaccgtgat attctgctga cttaacctgt ttaccattct gtgtaaatgc
      421 cttttcttta aagtgaccgt caggacggtc actgattacc cattctgaat tcagtgcaga
      481 attaaaatat gtgtgcaaaa taatttaatt cagacaggtt tttatttcac tatggctatc
      541 ccagaacaat cagtacaaat aataaaagac atatcacagc aacactccac actgacagcg
      601 caaaaataaa tgccgccttt gttatcttca tatacaagca acctgtcctg ataatcacca
      661 actcatgttt tattcttaac cattcgtcac aaatacaaca aaaaataaca gcagcaccga
      721 tatcacaata taagatacat tattcaacac ctgaatcccc cgccgcttct ctccccttta
      781 taaaacgccc cttccggaat ccagaccgct gcagtgcacg acgaagatga ccgtataccg
      841 tcttgtcatt ctgccccgtc tcttctgtaa tctccgcaat acttaaacct ctgtaatacc
      901 ggttcagtac cgctctgcgc tcttccagcg tcaacacacc tccggccgct ctcagattca
      961 gcatcgactt attcagaata cagcaggcct ctccggtaaa aagaatacga acaatatctg
     1021 tatactcatc atcacagaat acaaaataat atctgaatga cctgcctgac tgcacccttc
     1081 ttctgtctga tatggcatct tctatatatc tgattcgact gcttacg
//
LOCUS       DAPHCV010000351         1123 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.356, whole genome shotgun sequence.
ACCESSION   DAPHCV010000351 DAPHCV010000000
VERSION     DAPHCV010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.356"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            84..191
                     /locus_tag="RH033_004978"
     CDS             84..191
                     /locus_tag="RH033_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2994013.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(244..1002)
                     /locus_tag="RH033_004979"
     CDS             complement(244..1002)
                     /locus_tag="RH033_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HDV2994014.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          314 a          273 c          263 g          273 t
ORIGIN      
        1 gctgtaataa caaaaaatcc cttgatgcct gtcccttttg ttacactccg ttatcacgca
       61 caagagatat gcaggacact ggtatgccga ctaaacgctt tgataaaaaa cactggaaga
      121 tggtggtggt gctactggca atctgtggcg ctatgttgtt gctacgttgg gcagcaatga
      181 tttggggctg agcagtgaca aacgaagaca gccagaccgc ataacacggt ccggcagata
      241 attttacatg ctgtagccca gcgataaggt tgtccgacga tcggtatgtt ccggtgcaga
      301 ctctggaggt tcagagttcc atgtgacgtt gtaagccacc ttcagtccaa aatgttcatt
      361 aatagcaaca tttaatgcgc tttcggagtt cagcgttgtg tcttccgcgc caaagacgga
      421 aacaccctgc gtaaatttag cgttgtcagt caactgccat gcataagccc cggaggcata
      481 acccagcggc tgggtttcac tggcattatc ggtatatttg tcgtaacgca cacctggacc
      541 aaattcaaag cggaaactgt gtaccgggcc attcaaaaac tggcgaccat aacccgcggt
      601 caacacatcg cgctctcgat agccgttata acggtctgtc agccagcttg cctgcccaaa
      661 taaatagtca taatcagtta aattaaaacg gctacgcccg cccgccgcat atttttctga
      721 agaacgctca tcattagaag aagtattact ggcgttcccc cacagcgacc aggcagtggt
      781 ttgtccatac caggtcatgg tggtgtcagc cgtaagtgag gagcttttcg tattgcctga
      841 ttgtgcaaga tatcctgcgt tcagattacc ttcgaaaggt tttttagcgc tggcaggatc
      901 gtccatgaca gtaaaaacgg aatcatcggc agctgcattc agtgacgcaa acatgccccc
      961 cgccaacata acgatggcag gaactgtctt caaaagcttc atttatcaag agtccgtaca
     1021 acaaaaaaag agaccttcgc ggtcccggaa actttcttaa ggatcaaaga ttagcatccc
     1081 tggaaaggta acgaattata aaaaggcgcg aataacttag caa
//
LOCUS       DAPHCV010000352         1119 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.357, whole genome shotgun sequence.
ACCESSION   DAPHCV010000352 DAPHCV010000000
VERSION     DAPHCV010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.357"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..173
                     /locus_tag="RH033_004980"
     CDS             <1..173
                     /locus_tag="RH033_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV2994015.1"
                     /translation="EFLLSRPADLAQAREMVKESVAIYNHERPHLALKYKTPDDVHQA
                     FYRQKSVNLYQD"
     gene            complement(224..910)
                     /locus_tag="RH033_004981"
     CDS             complement(224..910)
                     /locus_tag="RH033_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HDV2994016.1"
                     /translation="MSFMAFVTCLTIAFVAGFVVARKLTLRKVASSVIISKSQPVPSS
                     ATPPAAKASTRSYTPQKTYRDSRWQERDELFHGMVAEGILLEGKEWLITRCEKIYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLNTIMTIARVPFVRASVTKLDEIPPVWDTRDKIRPL
                     AAATKGEPLH"
BASE COUNT          338 a          262 c          213 g          306 t
ORIGIN      
        1 atgagttttt actctcgcgt cctgcagacc tggcgcaggc ccgggaaatg gtcaaagagt
       61 ccgtggcaat ttataaccat gagcggccac atctggctct gaaatacaaa acgcccgatg
      121 acgttcatca ggcgttttac agacagaaaa gtgtcaactt atatcaggac tagtcaatat
      181 tgcacacacc ggggggatga gaagccccga ccggggtact tagttaatgc aacggttcac
      241 cctttgtcgc cgcggctaat gggcgaattt tgtcacgggt atcccagacc ggagggattt
      301 catccaattt agtgacagaa gctcgtacaa aaggcacccg tgcaatagtc ataatcgtat
      361 tcaaccaacg gtcacgaata atgcgatctg ccctttcatg actcggatca tccagctcaa
      421 taacacagac tattttgttg ttcttctttg aaaccagaac ataatccatt gccattccat
      481 tataaattgc aaagaaacgc tttctttctt cttcgctaaa ctcgggttgt tctggaaagt
      541 tgatcaagcg ccccagagag acctggcaat ggatatgaca ataatgtcca aaccatttct
      601 ccagattatc aaaatagatt ttttcacaac gcgtaattaa ccactctttt ccttccaata
      661 aaataccttc ggcaaccatt ccatgaaata actcatctcg ttcttgccaa cgagaatcac
      721 gataggtttt ctgcggtgta tagctccttg tcgacgcctt agcagcaggg ggagttgcag
      781 atgaagggac tggttgcgat ttagagatga tcaccgatga ggcaactttt cggagtgtca
      841 attttctggc aacaacaaaa ccggcgacaa atgcaattgt caaacaagtt acaaaagcca
      901 taaagctcat aattaatttt cacctatcca tgttgattat atttcattac caacgttact
      961 cgctgtagcg tgttacatgc caacaattca acgatcaata tatagttagt atcatgtttt
     1021 tattattaca accaccccta ttttgccatt gcggcttgcc ttccacaaaa aataaaaaca
     1081 caaaacatta gccaaagaat taattaagat taacctgac
//
LOCUS       DAPHCV010000353         1111 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.358, whole genome shotgun sequence.
ACCESSION   DAPHCV010000353 DAPHCV010000000
VERSION     DAPHCV010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.358"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            225..548
                     /locus_tag="RH033_004982"
     CDS             225..548
                     /locus_tag="RH033_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2994017.1"
                     /translation="MTFNTSGLTDITIVSGRKAVDRQGGNNFAFFHNASGNYRLRSSL
                     VTTSNNVSLSDIGLQGVKIAPINGSPLLPDSFAFRMKVNTNNGGSNLPAGEYTSTINV
                     TSNAL"
     gene            575..1081
                     /locus_tag="RH033_004983"
     CDS             575..1081
                     /locus_tag="RH033_004983"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2994018.1"
                     /translation="MNNFAKFIISSTIGFFSLSAIAATGPSINSKTLSITLHSPANIT
                     ANWVAESDLTPAKIMQPETLVGVISLSNTSIETATIRSTKPADASQQGVITFENGSGD
                     TFGTTISTNNSNVSIEKRTGESVISSTTQGAFLPSNLDLNVKTTSGNTTVSSGQYNAV
                     VSITTQIK"
BASE COUNT          360 a          206 c          202 g          343 t
ORIGIN      
        1 tgctatttaa tgttgaggat agtgatatga aaaaaagaag tttaatagga ctcttattcg
       61 ttatggtgtc gggtgctaat gctgctactg gtcctagtgt aaataatcag aggcttgatg
      121 tatcagtaac aagcacacct gtactcaatg ttagttttga accaaatcaa aatttatacg
      181 caggaactaa ctatggtatt tctgtagcag ctgatgctgg aatcttgact tttaatacct
      241 ctggattaac tgatattacc attgtttctg gacgaaaagc tgtcgacaga cagggaggga
      301 ataattttgc attttttcat aacgcatcag ggaactatcg acttcgttcc tctttggtaa
      361 ctactagtaa caacgtttct ttaagtgata ttggtcttca gggagttaaa atagctccta
      421 taaacggtag tcctttgctc cccgattctt ttgcatttag aatgaaggtc aatactaata
      481 atggtggatc gaatctacct gcaggtgagt atacatcaac catcaatgta acgagtaatg
      541 cactataata taaataactt agagattaaa aaacatgaat aactttgcaa aattcattat
      601 tagctcaaca attggattct tttccttaag tgctattgca gctacaggac cttcaattaa
      661 ttcaaaaaca ttaagtataa ctcttcactc tccagccaat attactgcaa actgggttgc
      721 agagtcagat ttaactcctg ctaaaataat gcagccagaa acgttggttg gtgttattag
      781 cctatccaat accagtattg aaacagcaac tattcgaagc accaagcctg ctgatgctag
      841 ccagcaaggt gttattactt ttgagaatgg tagcggtgac acatttggca ctaccatatc
      901 aacaaataac tccaatgtat ccattgaaaa gagaactgga gaaagtgtta ttagctcaac
      961 cactcagggc gcatttttac catcaaattt agatcttaat gtaaaaacaa catctggcaa
     1021 tacaactgta tcgtctggac aatataatgc tgtagtatca attaccacgc agattaaata
     1081 atcttctcaa gcctcctttt ttagaggagg c
//
LOCUS       DAPHCV010000354         1099 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.359, whole genome shotgun sequence.
ACCESSION   DAPHCV010000354 DAPHCV010000000
VERSION     DAPHCV010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.359"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            41..295
                     /locus_tag="RH033_004984"
     CDS             41..295
                     /locus_tag="RH033_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2994019.1"
                     /translation="MDEERVFSLSYEQLTRIAEKNIRECGLDNQSARCISELKASALL
                     WLWYELAIHGAPQNNHAQARERIDTDYQRLKKLIWSEGDS"
     gene            295..885
                     /locus_tag="RH033_004985"
     CDS             295..885
                     /locus_tag="RH033_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2994020.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLVPAAGLPADGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVCLEGDPLEVAQWLQACHDAGIEVRVQINESSVP"
     gene            complement(903..>1099)
                     /locus_tag="RH033_004986"
     CDS             complement(903..>1099)
                     /locus_tag="RH033_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2994021.1"
                     /translation="VLTGGLWGTSWGCAMWFIYWGPSGMVAGEAIIISITGGFLFGLL
                     TASFHWWRRKVNRLPPWDDV"
BASE COUNT          256 a          304 c          332 g          207 t
ORIGIN      
        1 atttttacct gtcagaacat tatcagatgg agcgtgcagg atggatgaag aaagggtgtt
       61 ttcactcagc tatgagcaac taacccgaat agcggaaaaa aatatccgtg aatgtgggct
      121 ggacaaccag agcgccaggt gtattagtga attgaaggcc tcagctctgc tgtggttgtg
      181 gtatgaactg gccattcatg gtgcgccaca gaacaatcac gcacaggccc gggaacgcat
      241 cgataccgat tatcagcgcc tcaaaaaact catctggtcg gagggtgaca gttaatgact
      301 cagagccgcc gaccgtcgcc actgcagcgg agagtgctga ttgtgctggc tgcccttgat
      361 gaaaaacggc cgggacccgt gctgacgcgg gacatagaac gggtgctgga gcgaagcggg
      421 gaagccccgg tctacgggcc aaacctgcgc gcctcctgcc gtcgtctgga ggatgccggc
      481 tggcttcgta ccctgcgcgc tccgaacctg cagctggcag tggagctgac ggacgccggc
      541 cgggccgtgg cacagcctct gctgctggca gaacaggacc gcctccgggc agaacagcgt
      601 gcggcagaag tcgtcgtact gccgctggtc ccggcagcag gtctgcccgc agacggcact
      661 tctgccactg atctggcggt agagctgaac ggcataacgt atcaggcctg ccgtggggat
      721 tttgtggtgc gtctcgacgg cagcacctgt ctgcagttat ggaataaaga aggcagggtg
      781 gtatgcctgg agggcgatcc gctggaagtg gcgcagtggc tgcaggcctg tcatgacgca
      841 ggaatagagg ttcgcgtaca gataaatgaa agtagcgtcc cgtaaaaaca tgcctgcatg
      901 gtttacacgt catcccatgg tggcagccgg ttaactttcc tgcgccacca gtggaaagag
      961 gccgtgagca gtccgaacaa aaaaccaccc gtgatactta taataatggc ctcacctgcc
     1021 accattcctg acggccccca gtagataaac cacattgcac atccccagga agtgccccat
     1081 aagccccccg tcagcaccg
//
LOCUS       DAPHCV010000355         1094 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.360, whole genome shotgun sequence.
ACCESSION   DAPHCV010000355 DAPHCV010000000
VERSION     DAPHCV010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.360"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..264
                     /locus_tag="RH033_004987"
     CDS             <1..264
                     /locus_tag="RH033_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV2994022.1"
                     /translation="SSYAKKTYNSGLQRASVNDEHFSLLIEISPIRSEKTIVALREYL
                     VYGYSKQESCDRNNVSNSYFCLCLKKLNYTHGIVSALKYFYQT"
     gene            complement(748..>1094)
                     /locus_tag="RH033_004988"
     CDS             complement(748..>1094)
                     /locus_tag="RH033_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV2994023.1"
                     /translation="ALMALDKFAGVWDEKYPQISKSWRTHWENLNTFFGYPPDIRKAI
                     YTTNAIESLNSVIRQAIKKRKVFPTDDSVRKVIYLAIQSASKKWSMPIQNWRLAMSRF
                     IIEFGDRLSEHL"
BASE COUNT          303 a          238 c          221 g          332 t
ORIGIN      
        1 tcttcttatg caaaaaaaac atataattca ggattacaac gagcaagtgt aaatgatgag
       61 cacttttcgc ttctgattga aatatcacca atcagaagtg aaaaaacaat agtggctctt
      121 agagaatatt tggtttatgg ttactcaaaa caagaaagct gtgacagaaa taatgtatcc
      181 aatagttatt tttgtttgtg tctgaaaaaa ctgaattata cccatggtat tgtatctgcc
      241 ctgaaatatt tctatcaaac ataaaatata atcataatat cgctttacct cccaaaatac
      301 agctgaactt tatgacttgg ctgataaagt gatactacga tgaaaactga atttgtttaa
      361 aaatcccggg cgtcagtcct ctgattgctg cagtattttt cagcaaaatt gatgccacat
      421 agttctctgg ccggtgacaa ttatcgacat ggttcggatt cgctccccgc cagcacagct
      481 ctggcggtaa acagcactac cttctgtggt caaaaatagc aaccagtgtc tgatagcttt
      541 cattctccgt gatgcgagtt tcgtaatgtg cggtgtgaaa aatgcgtatt ttcatggtag
      601 tagaacaccc aataacggcg tttttcaagc taattgtcgc cttttgtaat aaaattacat
      661 tatgctgttt cttttctgga ttcagataca ctgcctgaat atgttcccag tcgaccctgt
      721 aaataattct gtgtaactgc cgccgtatta aaggtgctcg ctaaggcggt caccaaactc
      781 gataataaaa cggctcattg ccagccgcca gttctggatt ggcatactcc atttttttga
      841 cgccgactgt atcgccagat aaatcacttt tcgcaccgag tcgtccgttg ggaacacctt
      901 gcgcttcttt atcgcctgcc gaatgacgct gttcagcgat tcgatggcat tcgtcgtgta
      961 gatggccttg cggatatccg gtgggtaacc gaagaaggtg ttgagatttt cccagtgtgt
     1021 gcgccagctc ttgctgattt gcgggtattt ttcatcccag acgcctgcaa acttatccaa
     1081 cgccatcaac gccg
//
LOCUS       DAPHCV010000356         1078 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.361, whole genome shotgun sequence.
ACCESSION   DAPHCV010000356 DAPHCV010000000
VERSION     DAPHCV010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.361"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            162..532
                     /locus_tag="RH033_004989"
                     /pseudo
     CDS             162..532
                     /locus_tag="RH033_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
     gene            579..956
                     /locus_tag="RH033_004990"
     CDS             579..956
                     /locus_tag="RH033_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDV2994024.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITQGKHPEAKQ"
BASE COUNT          263 a          315 c          267 g          233 t
ORIGIN      
        1 gttccgtcac ccggaaaacg agtacgacca gatggactca gacgccgctg tggatgaggc
       61 cctgctcatc aacgaataca cctttaccga agccggtcag cgcgccggct gacctgtcat
      121 cgttaaacca ttcttaacca cttttcagag aggattttat cgtgtcagac aaactctccg
      181 ggataactca tcccgacgac aatcacgccc ctggtggggg cttccctgca cagtgaggcc
      241 ctgttttggt gcccgtctgg tgcaggaggg taaccgcctg cattaccttg ccgaccgcgc
      301 cggtatcaga ggccgcttca gcgacgcaga tgcataccat ctggaccagg cctttccgct
      361 gctgatgaaa caactggagc tcatgctcac cagcggtgaa ctgaatcccc gccatcagca
      421 taccgtcacg ctgtatgcaa aagggctgac ctgcgaagcc gacaccctca gctcctgtgg
      481 ttacgtttat ctggctgttt atccggcaca ggcacccgca accacctcat aaccccgaag
      541 ctctcacctt tagcactacc agttacagag aacttaagat gaaaacatta tccgacacac
      601 atgtacggga ggtatctcgc tgcccgtctc ccgtcaccat ctggcagaca ctgctcatcc
      661 gactgctgga ccagcattat ggcctcacac tgaatgacac accgtttgcc gatgaacgtg
      721 tgattgagca gcatattgaa gctggcattt cactgtgcga tgcagtgaac tttcttgttg
      781 aaaaatacgc actggtgcgt accgaccagc cggaattcag tgcctgtacc cgttctcagt
      841 taataaacag catcgatatc ctccgggctc gcagggcgac cggcctgatg acccgcgaca
      901 attacagaac ggtaaataac attacccagg ggaagcatcc ggaggcgaaa caatgaaact
      961 gactctgacg ctggaagcag acagcgttaa cgtgcaggcg ctgaacatgg gacgaattgt
     1021 cgttgacgtc gatggtgtta atctcgctga actgattaac gtggtctgcg acaatggc
//
LOCUS       DAPHCV010000357         1056 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.362, whole genome shotgun sequence.
ACCESSION   DAPHCV010000357 DAPHCV010000000
VERSION     DAPHCV010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.362"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            183..>1056
                     /gene="rluB"
                     /locus_tag="RH033_004991"
     CDS             183..>1056
                     /gene="rluB"
                     /locus_tag="RH033_004991"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HDV2994025.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEI
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNNG"
BASE COUNT          261 a          248 c          304 g          243 t
ORIGIN      
        1 taaccataaa gatagtgtag tatgttgcgc ctcaaagcca ggccgataaa cgtcgagtcg
       61 tttacttaag gcctgaagag ttcaaacaga ctttactatc agtgagtcag atgtaaattc
      121 tgactcactg ttgcacaaaa gcatatcaga cgcctgggaa ggcgacacca aaaggaagct
      181 ctatgagcga aaagctacag aaagtgctgg cgcgtgccgg ccacggttct cgccgtgaaa
      241 tcgaatctat tattgaagcc ggtcgtgtga gtgttgatgg caaaattgct aaactcggcg
      301 atcgcgttga aattacccct ggactgaaaa tccgtatcga tggtcacctg atttcggtac
      361 gtgagtccgc tgaacaaatt tgtcgcgttc tggcctatta caaaccggaa ggtgagttgt
      421 gtacccgtaa cgatccggaa ggacgtccaa cggtgtttga ccgtctgcca aaactgcgtg
      481 gcgcacgctg gattgccgta ggtcgtctgg acgttaacac ctgtggtctg ctgctgttca
      541 ctaccgacgg tgaactggca aaccgtttaa tgcatccaag ccgtgaagtt gaacgtgaat
      601 acgccgtgcg cgtatttggt caggttgacg acgcgaaact gcgtgatttg agtcgtggcg
      661 tgcagctgga agatggtccg gcagccttta aaaccatcaa gttcagcggc ggcgaaggga
      721 tcaaccagtg gtacaacgtt actctgaccg aaggccgtaa ccgtgaagtt cgtcgtctgt
      781 gggaagcggt tggtgtgcag gtaagccgcc tgatccgtgt tcgttacggt gatatcccac
      841 tgccgaaagg tctgccacgc ggtggctgga cggagctgga cctcgcccag actaactatc
      901 tgcgcgaact ggtggagcta ccgccagaaa ccagctctaa agtcgctgta gaaaaagacc
      961 gtcgtcgtat gaaggcgaat cagattcgtc gtgcggtgaa acgtcacagt caggtgagcg
     1021 gcggtcgtcg ttctggtgga cgtaataata acggtt
//
LOCUS       DAPHCV010000358         1031 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.363, whole genome shotgun sequence.
ACCESSION   DAPHCV010000358 DAPHCV010000000
VERSION     DAPHCV010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.363"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            19..719
                     /locus_tag="RH033_004992"
                     /pseudo
     CDS             19..719
                     /locus_tag="RH033_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            771..>1031
                     /locus_tag="RH033_004993"
     CDS             771..>1031
                     /locus_tag="RH033_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV2994026.1"
                     /translation="MSRKTQRYSKEFKAEAVRTVLENQLSISEGASRLSLPEGTLGQW
                     VTAARKGLGTPGSRTVAELESEILQLRKALNEARLERDILKKA"
BASE COUNT          283 a          252 c          250 g          246 t
ORIGIN      
        1 ggcttttgag gttatctcat ggccagagtt aatattcatt gtccccgttg tcagtcagct
       61 caggtttacc gccatggtca gaaccctaaa ggccgtgaca gatttcgctg ccgtgactgc
      121 caccgtgtgt ttcagctcac ttatacttat caagcacgta agccgggtat gaaagagctg
      181 ataactgaaa tggcctttaa tggtgccggg gttcgcgata ccgctaggac actgaaaatt
      241 gatattaaca ccgtcatccg gactttaaaa aactcgcgcc aaagcgaata acttcttcgt
      301 ctgttgccca tgctgatgtg gcgcttattt gcgagcttga tgagcaattg gagctgcgtt
      361 ggcagtaaag cccggcaaca ctggctctgg tacgcgtaca acaccaaaac aggcggtgta
      421 ctggcctaca cttttggtcc ccgaaccgat caaacgtgcc gggagctact ggcactgctt
      481 acacccttca acatcggcat gctgaccagc gatgactggg cagctatggc cgggaggtgc
      541 cgaagaataa gcatctgacc ggcaaaatat tcacccaacg cattgagcgt aataatctga
      601 cgctacgcac ctgcattaag cggttggctc gtaaaacaat ctgcttctca cgctcagttg
      661 agatccacga aaaagtaatc ggagcgttta tcgaaaaaca catgtttgag gtagcctgag
      721 tttaacggac actccttcct gaaatagaat ggcatcagaa ggagctaata atgagcagaa
      781 aaacccaacg ttactctaaa gagttcaaag ccgaagctgt cagaacggtt cttgaaaatc
      841 aactttcgat cagtgaaggc gcttcccgat tatctcttcc tgaaggcact ttaggacaat
      901 gggttaccgc cgccagaaaa gggctcggta ctcctggttc ccgcacggtg gctgaactgg
      961 aatctgaaat tctgcaactg cgtaaggcgt taaatgaagc tcgccttgag cgagatatat
     1021 taaaaaaagc a
//
LOCUS       DAPHCV010000359         1027 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.364, whole genome shotgun sequence.
ACCESSION   DAPHCV010000359 DAPHCV010000000
VERSION     DAPHCV010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.364"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            11..985
                     /locus_tag="RH033_004994"
     CDS             11..985
                     /locus_tag="RH033_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV2994027.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTVLLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAQPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT          286 a          243 c          246 g          252 t
ORIGIN      
        1 ggctgttgcg atgacaaaat tacaacttaa gtaccgggag ttaaaaatta tctcggtaat
       61 cgctgccagt gaaaatatca gccatgccgc gactgtactt ggcatcgcac aggccaacgt
      121 cagcaaatat cttgctgatt ttgaatcaaa agtgggttta aaagtctttg accgaaccac
      181 ccgacagctg atgctcacac cttttggcac cgtgctgtta ccgtatatca acgacatgct
      241 ggacagaaac gagcagctta ataattttat tgcagattat aagcatgaga aacgtggtcg
      301 ggtcactatc tatgcaccaa ccggcataat tacctattta tccaaacatg taatcgataa
      361 aattaaagat atcggtgata tcaccttatc gctaaaaacc tgcaatcttg agcgtaatgc
      421 cttttatgaa ggcgtcgaat ttcccgatga ttgtgatgtt ttaatcagct acgcccaacc
      481 aaaagatgaa tcgctggttg ccagttttat tacccaatat gccgtaaccg cttatgccag
      541 ccagcgctat cttgagaaac atcccattag ccgtcctgac gaactcgaac atcactcctg
      601 tattttgatc gactcgatga tgatcgacga cgcgaatatc tggcgcttca acgtcgccgg
      661 gagtaaagag gtgcgggatt atcgcgtgaa gggaaattac gtttgcgaca acacgcaatc
      721 tgcgctggag ctggcacgaa atcacctggg gattgtgttt gcgccagaca aaagtgtgca
      781 gagcgacctg caagacggca cgctggtgcc ctgctttcag cacccgtatg aatggtggct
      841 ggatctggtg gctatcttcc gtaaacggga ataccagccc tggcgggtcc agtatgtgct
      901 ggatgaaatg ctgcgtgaaa ttcgccacca gcttgctcag tcgcagcaac tgcggcctga
      961 acaagcggcg gaaagcgaag attaatgatc gagataaagg taatgcaccc agctggtcat
     1021 gcgtaac
//
LOCUS       DAPHCV010000360         1008 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.365, whole genome shotgun sequence.
ACCESSION   DAPHCV010000360 DAPHCV010000000
VERSION     DAPHCV010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.365"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            222..1008
                     /locus_tag="RH033_004995"
                     /pseudo
     CDS             222..1008
                     /locus_tag="RH033_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
BASE COUNT          198 a          262 c          312 g          236 t
ORIGIN      
        1 cccaggccgt gccggcacgt taaccgggct gcatccgatg caagtgtgtc gctgtcgccg
       61 gcctcctcac ccggtcacgt ttcgtcgttc ctcctccacg cgctgcggct tcggggccgc
      121 acctgcattc gtatgcggtc gcccggttac aggtgcggca cggcctgatg gaggccgcat
      181 gtgagaggag aattcccatg ccaaactggt gctcgaatcg tatgtatttt tctggtgaac
      241 cggcacagat cgctgagatt aaacgactgg ccagcggtgc agtcactccg ctttatcgcc
      301 gcgccacaaa tgaaggtatt cagctgtttc tggccggaag tgccggactt ctgcagacca
      361 ctgaagatgt gcggtttgaa ccatgccccg gactgacggc tgccgggcgt ggcgttgtat
      421 cgccggagaa tatcgcgttc acccgctggc tgacacacct gcaggacggt gtacttctgg
      481 atgagcgaaa ctgcctgatg ctgcatgaac tctggctgca gagcggtact ggccggcgtc
      541 gctgggaaga attaccggat gatgccaggg aaagcattac cgctcttttc accccaaaaa
      601 gaggtgactg gtgcgacatc tggagtaacg aggatgtatc ggtgtggtgg aaccgtctgt
      661 gtgacaacgt cctgccggaa aaaccatgcc gtttgacctg ctgtcggttc tgtccactcg
      721 cctggatgtt gaagtgaatg gctttaacgg tggtgttctg aacggtgttc cttctgcata
      781 tcactggtat acggaacagt atggcgtgaa gtggccttgt gggtatgagg tgaatatcag
      841 cagtcaggga gacaacttca ttcaggttga tttcgacacg ccgtggtgtc agccggaaag
      901 cgacgttatt gcagtattaa gccgccgttt cagttgcacg ctggagcact ggtacgccga
      961 gcagggctgc aatttctgtg gctggcagcg atatgagcgt ggagagct
//
LOCUS       DAPHCV010000361         1004 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.366, whole genome shotgun sequence.
ACCESSION   DAPHCV010000361 DAPHCV010000000
VERSION     DAPHCV010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.366"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            218..487
                     /locus_tag="RH033_004996"
     CDS             218..487
                     /locus_tag="RH033_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2994028.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRDEMSRKVAGK"
     gene            484..765
                     /locus_tag="RH033_004997"
     CDS             484..765
                     /locus_tag="RH033_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDV2994029.1"
                     /translation="MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLA
                     GQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVYILRVLHTAQKWP"
     gene            811..>1004
                     /locus_tag="RH033_004998"
     CDS             811..>1004
                     /locus_tag="RH033_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HDV2994030.1"
                     /translation="MNNDELVTRRAQAIAEDRCFSKGRLRDEFRMKPAPGAEPVKWYK
                     NTYGGRFAVYRIADCVPMRE"
BASE COUNT          258 a          216 c          301 g          229 t
ORIGIN      
        1 ctatcatggc tgaaagcggg tatggcttag cagggcgggg atgggacagg tgaaatctat
       61 caatcagtac cggcttacgc cgggcttcgg cggttttact ccggtatcat atgcaacaat
      121 ggagtgccgc cttccatgcc gctgacgcgg catatcctgg tgacgatatc tgaatcgtta
      181 tatactgtgt atatacgtag taatgacgag gtgataaatg gcacaggtta atatgagttt
      241 aagaatcgac gctgaactga aggatgcttt tatggccgct gcaaaaagca tggatcgtaa
      301 tggctctcag ttaatccggg attttatgcg ccagaccgtt gaacggcagc ataatacctg
      361 gttccgcgac caggttgcgg caggacgtca gcaactcgag cacggcgatg tgcttcccca
      421 tgacatggtc gaatcatctg ctgcagcatg gagggatgaa atgagcagga aggtcgccgg
      481 taaatgatgg agatattctg gaccatgctg gccagccagg acaggaagcg cattcgtgag
      541 tacattgcgg agcagaacct gatggccgcc attgaactgg acgaacggat tggttattca
      601 gccagcagcc tggccgggca gccgtataaa ggccgcaacg gtcgtgtgga aggtacccgg
      661 gaactggtta ttcacccaca ttttgttctg gtttatgagg tcgacagcca gtggggaaaa
      721 gtgtatatcc tgcgagtatt gcatactgca cagaagtggc catagagggg ctgtccggat
      781 ataaaaacgt ctgacggaga tgcaagaacc atgaataatg atgagctggt caccagacgt
      841 gcgcaggcga ttgccgaaga caggtgtttc tcgaaaggac gtctgcgcga tgaattcaga
      901 atgaagccag cccccggtgc tgagccggtt aagtggtata aaaatactta tggtggcagg
      961 ttcgctgtat accgaattgc agactgtgtt ccgatgcgtg agaa
//
LOCUS       DAPHCV010000362          985 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.367, whole genome shotgun sequence.
ACCESSION   DAPHCV010000362 DAPHCV010000000
VERSION     DAPHCV010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.367"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            39..497
                     /locus_tag="RH033_004999"
                     /pseudo
     CDS             39..497
                     /locus_tag="RH033_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907654.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            571..828
                     /locus_tag="RH033_005000"
                     /pseudo
     CDS             571..828
                     /locus_tag="RH033_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(841..>985)
                     /locus_tag="RH033_005001"
     CDS             complement(841..>985)
                     /locus_tag="RH033_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDV2994031.1"
                     /translation="FPKKTCLAQYTQHELDLVAAQLNNRPRKTLKFKTPKEIIERGVA
                     LTD"
BASE COUNT          288 a          213 c          241 g          243 t
ORIGIN      
        1 agcagctctg gtgaaggatt ttccagcgtc atgtaaccgt ggcggaactc aactttacag
       61 gaattgtggg tggcgtggat acacataaag atctgcacgt tgccgctgtc gtagatcaga
      121 acaataaaat tatggggact cagtttttct ccacaacacg gcaaggttac cggcagatgc
      181 tggcatggat gacttcgttt ggggcattaa agcgaattgg tgttgagtgt acaggcatct
      241 atggatcagg tctgcttcac tatttacaga atgccgggtt agaagttctt gaggtgattg
      301 cgccagatcg gatggagcga cgcaaacggg gtaaaagtga cacgattgat gctgaatgtg
      361 ccgctcacgc cgcattctcc ggaataagaa ccgtcacacc caaaacgcgc aatggcatga
      421 ttgagtctct gcgggtatta aaaacttgcc gaaaaacagc aatatcagcc cgcagagtcg
      481 ctctccagat tatccatttg aagtggttta ctgaatttgg ccacctgaac agaggtgata
      541 tgctcacctc agaacaacac aggtgccata atgaaaaaaa gaaatttcag cgcagagttt
      601 aaacgcgaat ccgctcaact ggtcgttgac cagaattaca ccgtggcaga tgcagccagc
      661 gctatggatg tcggcctttc cacaatgacg cgatgggtga aacaattacg tgatgagcgg
      721 cagggaaaaa caccaaaagc ctcccccatt accccggaac aaattgaaat ccgtgagctc
      781 aggaaaaagc tacaacgtat tgaaatggaa aatgaaatat taaaaaaggc tactgtagat
      841 tcaatctgtc aatgcaacac ccctttcaat tatctctttc ggtgttttga acttcagtgt
      901 ctttctcggt ctgttgttta gctgagcagc aaccagatcc agttcatgtt gagtatattg
      961 ggcaagacat gtctttttag gaaag
//
LOCUS       DAPHCV010000363          975 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.368, whole genome shotgun sequence.
ACCESSION   DAPHCV010000363 DAPHCV010000000
VERSION     DAPHCV010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.368"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..117)
                     /locus_tag="RH033_005002"
     CDS             complement(<1..117)
                     /locus_tag="RH033_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDV2994032.1"
                     /translation="MELKKLMEHISIVPDYRQAWKVEHKLSDILLLTICAVIS"
     gene            255..671
                     /locus_tag="RH033_005003"
                     /pseudo
     CDS             255..671
                     /locus_tag="RH033_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(797..>975)
                     /locus_tag="RH033_005004"
     CDS             complement(797..>975)
                     /locus_tag="RH033_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2994033.1"
                     /translation="NSYFKETTKFIDDKNITHRLAEIRRVITENFNTDLGDDDMDDIE
                     REIEKIKCWEKPGD"
BASE COUNT          296 a          208 c          205 g          266 t
ORIGIN      
        1 agaaataacg gcacaaatag tcaacagtag aatatccgac aatttatgtt ccactttcca
       61 ggcttgtctg taatcgggga caatagaaat atgttccatc aattttttaa gttccatttt
      121 gttctcctta attatgtaag gagtatttga tcatgtataa gcaataaaaa acagctttag
      181 gtaatgagga atatctcaaa tcttaaacat aaaatgccaa ttatttagta caaaaaagta
      241 gcgttacgtg atctttccct aaaacgcaga cccagttgtg catagtacac atggtacgca
      301 acagcctgcg gttcgtgacg tggaaagact ataaagcggt aacccgtgac ctgaaacagg
      361 tttaccgcgc ggccacggaa gaaggcgtgt tgcaggcgct ggatgcgttc gggaaggaat
      421 gggatgcacg gtacccgcag ataagtcgca gctggcgggc aaactgaccg aatgttgcca
      481 ccttcttcgt gtacccggag gcgatccgaa aggttatcta caccactaac gcaatcgaat
      541 cgctgaatag cgtcatcaga catgcgataa agaaaaggaa agtgttcccg acagatgaat
      601 cggtgaaaaa agtggtgtgg ctggctatcc agtcagcctc acagaaatgg acgatgccac
      661 ttcaggacta gcggatagcc atgagtcgct ttattatcga gttcggtgac cgccttgacg
      721 gtcacttctg agaaaggcat ttacacagaa tgatggacag gctcaacggg aaggctacat
      781 gccttcccat tttatattaa tcacccggct tctcccaaca ttttatcttt tctatttctc
      841 tctcaatatc atccatatca tcatctccaa gatcggtatt aaaattttca gtaataaccc
      901 ttcttatttc agccaaccga tgtgtaatat ttttatcatc aataaattta gtggtttctt
      961 taaagtaaga attca
//
LOCUS       DAPHCV010000364          963 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.369, whole genome shotgun sequence.
ACCESSION   DAPHCV010000364 DAPHCV010000000
VERSION     DAPHCV010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.369"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>963)
                     /locus_tag="RH033_005005"
     CDS             complement(<1..>963)
                     /locus_tag="RH033_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HDV2994034.1"
                     /translation="AAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQGSGRTIFAEA
                     AYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNY
                     VKAIRYLQGIEHNGDPFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIA
                     IRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDI
                     YGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQYSYGADPVRDGVS
                     TGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQAR"
BASE COUNT          228 a          265 c          249 g          221 t
ORIGIN      
        1 gtcgtgcctg atatttcaga gagagtttga ctgccggata cggtccgggc agagtgacat
       61 agcaggtcag atccggcaga gactgaatgt cggaataact gaccagcgtc tgacgctcca
      121 tatccttacc ggtcgatacc ccgtcacgta ccgggtcagc accgtaggaa tactgctcgc
      181 tggctttcag gtgctctttc tcaccaatct caccggcagc gaactctgca atcttatggc
      241 tcggggaacg gaaaaaagca cgggtgttca tgacgtcaaa cagcgtggcc gccgctttct
      301 caccgtagat atcttccagc tgggcatagg actggatacc aaacacataa cagccaccga
      361 acttacgggc ttccggcagg atctccacca gatccggcag tttgtgtaac gtgggtaact
      421 catcacagaa aaaccacaca cgacggttac ggttttctcc cattgccagc agaccacgaa
      481 tggcaatgga cagccacatg gagatcacag gcttcaggga ggcatgggtg tcggcgttcg
      541 acgaaataaa cagccagccg tttttctgat cttcccggac accacgcatc cagtcacgga
      601 tggtgaaggg atcaccgtta tgctcaatcc cctgcaggta acggatggct ttcacgtagt
      661 tggtcagcac agcacggatg gaaatcgccg ttttctcaat tttctcttcc accaggttgg
      721 ccgccggtga attacgcagg aaggtacgca gtttttcaat tttgatggaa agcagtgtgt
      781 ccaccagttt gctgtagctg cggttggggt cattacgcat caggtacgcc gcttccgcaa
      841 aaatggtacg tcctgaaccc tgccagaacg ggtcctcttt tgtacccata gggatcagcg
      901 tgttcgcggt attatcaaaa tccggctgtg tcaggcactc cttccacaaa tcccatgcgg
      961 cac
//
LOCUS       DAPHCV010000365          955 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.370, whole genome shotgun sequence.
ACCESSION   DAPHCV010000365 DAPHCV010000000
VERSION     DAPHCV010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.370"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..893
                     /locus_tag="RH033_005006"
     CDS             <1..893
                     /locus_tag="RH033_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDV2994035.1"
                     /translation="VFCTGVAEKYALIEQWRQQFPIEAMCQVFGVSRSGYYNWVQHEP
                     SDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRCK
                     QERKFRATTNSNHNLPVAPNLLNQMFAPTATNQVWGADLTYVATQEGWLYLAGIKDVY
                     TCEIVGYAMGERMTKELTGKALFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQFG
                     LKTSMSRKGNCYDNAPMESFWGTLKNESLSHYRFNNRDDAISVIREYIEIFYNRQRRH
                     SRLGNISPAAFREKYHQMTA"
BASE COUNT          270 a          224 c          249 g          212 t
ORIGIN      
        1 gcgtattttg cacaggagtc gctgaaaaat acgcgttaat cgaacaatgg cgacaacaat
       61 ttcccattga agcgatgtgt caggtatttg gtgtatccag gagcggttat tacaactggg
      121 tacagcatga accctcagac agaaaacaaa gtgatgagcg gctaaaactg gagattaagg
      181 tggcacatat ccgcactcgc gaaacatatg gaacccggcg gctccagacg gagctggcag
      241 agaatggcat catcgttggt cgtgaccgac tggctcgtct tcgtaaggag ctaaggctac
      301 gctgtaagca ggaacgcaag ttcagagcga ctacgaactc gaaccacaat ttgccagttg
      361 cgccaaatct gctgaaccag atgttcgctc ctacagcaac aaatcaggtc tggggggcgg
      421 acctgacgta tgttgccaca caggagggat ggttgtacct cgctggcatc aaagatgttt
      481 atacgtgcga aattgtcggc tacgccatgg gagagcgcat gacaaaagag ctgacaggta
      541 aagccctgtt tatggcgctc aggagtcagc gcccacctgc cgggctaatc caccactctg
      601 atcgaggttc acagtactgc gcatacgatt accgggtcat acaggagcag tttggtctga
      661 aaacatcaat gtcgcgtaaa ggtaactgtt acgacaacgc tccgatggaa agcttctggg
      721 gaacgctgaa aaatgagagc ctgagccact atcgttttaa taaccgggat gatgccatct
      781 cagtaatacg ggaatacatt gagattttct acaatcgtca gcgtcgtcac tctcgtctgg
      841 ggaatatctc cccggcagcc ttcagggaaa aatatcatca gatgactgct taaaaaaaga
      901 acaaatggta gtgtccgcta ttgccagtac acctcagttc tactaattgg atgcg
//
LOCUS       DAPHCV010000366          928 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.371, whole genome shotgun sequence.
ACCESSION   DAPHCV010000366 DAPHCV010000000
VERSION     DAPHCV010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.371"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..840
                     /locus_tag="RH033_005007"
     CDS             <1..840
                     /locus_tag="RH033_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HDV2994036.1"
                     /translation="TPLYRRATNEGIQLFLAGSAGLLQITENIRSEQCPGVTAAGRGA
                     VSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSGTGQRRWEELPDDVRETITVHF
                     TAKRGDWCDIWGNEDVSVWWNRLCDNVLPEKTMPFDLLTVLPTRLDIEVNGFNGGVLN
                     GVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDFDTPWCQPESDVVAELSRRFGC
                     TLEHWYAEQGCNFCGWQLYERGELVDVLWGELEWSSPTDDDELPEVTGPAWIIDNVAH
                     YGG"
BASE COUNT          203 a          219 c          289 g          217 t
ORIGIN      
        1 acaccgcttt atcgccgcgc tacaaatgaa ggtattcagc tgtttctggc cggaagtgcc
       61 ggacttctgc agatcactga aaatatccgc tctgagcagt gccccggagt gacggctgcc
      121 ggacgtggtg ctgtatcacc ggaaaatatc gcgttcaccc gctggctgac acacctgcag
      181 aacggtgtac tgctggatga gcaaaactgc ctgatgctgc atgaactctg gctgcagagc
      241 ggtactggac agcgtcgctg ggaagagtta ccggatgatg tcagggaaac tatcactgtg
      301 catttcaccg caaaaagagg tgactggtgt gacatctggg gtaacgagga tgtatcggtg
      361 tggtggaacc gtctgtgtga caacgtactg ccggaaaaaa ccatgccgtt tgacctgctg
      421 acggttttgc cgacccgcct ggatattgaa gtgaatggct ttaacggcgg tgttctgaac
      481 ggtgttcctt ctgcatatca ctggtataca gaacggtatg gcgtgaagtg gccttgtggg
      541 tatgacctga atatcagcag tcagggggaa aacttcattc aggtagattt cgacacgccg
      601 tggtgtcagc cggaaagcga cgttgttgca gaattaagcc gccgtttcgg ctgcacgctg
      661 gagcactggt acgccgaaca gggctgcaat ttctgtggct ggcagttgta tgagcgcgga
      721 gagctcgttg atgtgctgtg gggggaactg gaatggtctt ccccgacaga tgatgatgag
      781 ctgccggaag tcaccggacc tgcgtggata atcgacaatg tggcgcatta tggcggatga
      841 agtatgacgg agacgggcgg gcaaccgccc gtttcttttc cgacaaagga tgtcgccgtg
      901 ctcctctttt tactgcgccg gctgacgc
//
LOCUS       DAPHCV010000367          898 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.372, whole genome shotgun sequence.
ACCESSION   DAPHCV010000367 DAPHCV010000000
VERSION     DAPHCV010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.372"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            242..844
                     /gene="fimB"
                     /locus_tag="RH033_005008"
     CDS             242..844
                     /gene="fimB"
                     /locus_tag="RH033_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HDV2994037.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IRDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          265 a          166 c          198 g          269 t
ORIGIN      
        1 ttcgattcgt atcagattga taaatatata aaggtacata gcatgcaaga gcatggcgtt
       61 tgtatggcaa agttattata agtaactatc gctactccat ttaagttcgc tcagaagaac
      121 tggtccactt acgttagtta ttaagcaaac gttcgctttt ataaacataa tcaggataaa
      181 aatgttggat tattgctaac ccagcacagc tagtgcgcgt ctgtaattat aagggaaaac
      241 gatgaagaat aaggctgata acaaaaaaag gaacttcctg acccatagtg aaatcgaatc
      301 actccttaaa gcagcaaata ccgggcctca tgcagcacgt aattattgtc tgactttgct
      361 ttgttttatt catggtttcc gggcgagtga aatttgtcga ttgaggattt cggatattga
      421 tcttaaggca aagtgtatat atatccatcg attaaaaaaa ggcttttcaa caacgcaccc
      481 gctattaaac aaagaagttc aggctttaaa aaactggttg agtatccgta cttcttatcc
      541 gcatgctgag agcgagtggg tatttttatc acgtaagggg aatccgcttt ctcggcaaca
      601 gttttaccat attatctcga cttctggtgg taatgccggg ttgtcactgg agattcatcc
      661 gcacatgtta cgccattcgt gtggttttgc tttggcgaat atgggaatag atacgcgact
      721 tatccgggat tatcttgggc atcgcaatat tcgtcatact gtctggtata ccgccagcaa
      781 tgcagggcgt ttttacggca tctgggatag agccagagga cgacagcgtc acgctgtttt
      841 atagagatag ttgagatacc agggatggtg ttaaaaacta cattattctt acgatata
//
LOCUS       DAPHCV010000368          893 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.373, whole genome shotgun sequence.
ACCESSION   DAPHCV010000368 DAPHCV010000000
VERSION     DAPHCV010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.373"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>893
                     /locus_tag="RH033_005009"
     rRNA            <1..>893
                     /locus_tag="RH033_005009"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          242 a          195 c          275 g          181 t
ORIGIN      
        1 cgaatgctga cataagtaac gataaagcgg gtgaaaagcc cgctcgccgg aagaccaagg
       61 gttcctgtcc aacgttaatc ggggcagggt gagtcgaccc ctaaggcgag gccgaaaggc
      121 gtagtcgatg ggaaacaggt taatattcct gtacttggtg ttactgcgaa ggggggacgg
      181 agaaggctat gttggccggg cgacggttgt cccggtttaa gcgtgtaggc tggttttcca
      241 ggcaaatccg gaaaatcaag gctgaggcgt gatgacgagg cactacggtg ctgaagcaac
      301 aaatgccctg cttccaggaa aagcctctaa gcatcaggta acatcaaatc gtaccccaaa
      361 ccgacacagg tggtcaggta gagaatacca aggcgcttga gagaactcgg gtgaaggaac
      421 taggcaaaat ggtgccgtaa cttcgggaga aggcacgctg atatgtaggt gaagcgactt
      481 gctcgtggag ctgaaatcag tcgaagatac cagctggctg caactgttta ttaaaaacac
      541 agcactgtgc aaacacgaaa gtggacgtat acggtgtgac gcctgcccgg tgccggaagg
      601 ttaattgatg gggttagcgg taacgcgaag ctcttgatcg aagccccggt aaacggcggc
      661 cgtaactata acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacctgcacg
      721 aatggcgtaa tgatggccag gctgtctcca cccgagactc agtgaaattg aactcgctgt
      781 gaagatgcag tgtacccgcg gcaagacgga aagaccccgt gaacctttac tatagcttga
      841 cactgaacat tgagccttga tgtgtaggat aggtgggagg ctttgaagtg tgg
//
LOCUS       DAPHCV010000369          885 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.374, whole genome shotgun sequence.
ACCESSION   DAPHCV010000369 DAPHCV010000000
VERSION     DAPHCV010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.374"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..502
                     /locus_tag="RH033_005010"
                     /pseudo
     CDS             1..502
                     /locus_tag="RH033_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724480.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS domain-containing protein"
     gene            596..853
                     /locus_tag="RH033_005011"
     CDS             596..853
                     /locus_tag="RH033_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2994038.1"
                     /translation="MEAFPDAQKVRGIGSQDAAGIRKKHKMEQFKKRDGTVRYRKGYP
                     IDSNTGRVYGHDDHKGTGHGSLPHINIKRSDGTMVRIDIDG"
BASE COUNT          256 a          177 c          267 g          185 t
ORIGIN      
        1 acggaaaaac agcacatcag ccctgacggt aaaccgtaca accggacggc atttttatgg
       61 gacggaatgc ggctggcgca ggagagcagg ccggagggaa cagacagcct gtacatctac
      121 agggacccgg ggagttatga accgctggcg cgggtggata aggcgggcaa agagggcccg
      181 aaccggatac tgtattttca cacggatgtg aacggcgcgc cggaagagat gacggacagt
      241 gacgggaaaa tcgtgtggga gacggggtat caggtctggg gtaacacgat acaggagaag
      301 gaccacggcg gggtggcgca gaacctgcgc taccagggac agtacctcga cagggaaacg
      361 ggactgcatt acaatctgca caggtactat gatccggatg tcgggcggtt catggtaacg
      421 gatccgatag ggttagcggg ggggaataaa cctctaccaa tacgcgccga atccgctctc
      481 ttggatcgat ccgctgggac tgactgcatg ttccaaaaac gcgaaatctt tgcgagaggg
      541 gcctcaagga accgttgtca cagttaaatc aaaacaggaa gcccatgatt tattgatgga
      601 ggcctttcct gatgctcaaa aagtaagagg gatcggttcg caggatgccg ccggtattag
      661 aaaaaaacat aagatggaac aatttaagaa aagagatggt actgtccgtt ataggaaagg
      721 ttaccctatt gattctaata ctggtcgtgt atatggtcat gatgaccata aaggcacagg
      781 gcatggttct ttgcctcata taaatattaa acgttctgat gggacaatgg tgaggataga
      841 tatagatggt tgatttgtct attgaaaaat taattgttgc tatag
//
LOCUS       DAPHCV010000370          884 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.375, whole genome shotgun sequence.
ACCESSION   DAPHCV010000370 DAPHCV010000000
VERSION     DAPHCV010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.375"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..367)
                     /locus_tag="RH033_005012"
     CDS             complement(<1..367)
                     /locus_tag="RH033_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV2994039.1"
                     /translation="MDKPTDWRSGTRRIFSNEFKLHMVELASKPNANVAQLAREHGVD
                     NNLIFKWLRLWQREGRISRRMPPTIVGPTVSQSFPASPTLVPVELIDTPRCATDAPAP
                     EALSVACAASCHVEFHYGKM"
     gene            complement(650..>884)
                     /locus_tag="RH033_005013"
     CDS             complement(650..>884)
                     /locus_tag="RH033_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2994040.1"
                     /translation="CVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFFHGKKFC
                     EACGTGIYCRAVCTKSVYSYQPDLFLDCGGAST"
BASE COUNT          251 a          218 c          209 g          206 t
ORIGIN      
        1 tcattttacc gtaatggaat tccacatggc aggaagctgc acaagcaact gataatgcct
       61 ccggagcagg agcatctgta gcacagcgcg gggtgtcgat aagttccact ggaaccagag
      121 tcggagaggc cggaaaagat tgtgatactg tagggcctac aatagttgga ggcattctac
      181 gagaaatacg tccttctctt tgccagaggc gtagccattt aaaaatcagg ttgttatcaa
      241 cgccatgttc ccgggccagt tgtgcaacat tggcatttgg tttcgaagcc agttcaacca
      301 tatgaagttt aaattcatta gaaaatatcc ggcgggttcc ggagcgccag tctgtaggtt
      361 tatccatcaa tagaactccg tctaatttag aaggagctct aatttagtct gcaaggagca
      421 ggtgttacag acggcattgg ctttacgctt acacataaat gaaggacatc actaaataaa
      481 tgattccgca ccacactacc aatcaatacc ccgggtttta ttcaggtaag caggactgcg
      541 gatatccggt gtcgcgcctt tgtgtcacga acggggtggg tgcgaaacac cggataaaat
      601 acaggttggc aatacctctg aacgccctgc gcagagcggg gatttcggat taagtactcg
      661 cacctccgca gtccaggaac aagtctggct ggtagctgta aacagacttc gtacataccg
      721 ctctgcaata aatccccgta ccacaggcct cacagaactt cttcccatga aaaaactgct
      781 cacacatcac acaatgccac tccagcacga ccggtaatgg cgataaaaac atcgccatat
      841 cctcaatgta agggtgggac ttttccggat tcagcaccac acag
//
LOCUS       DAPHCV010000371          880 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.376, whole genome shotgun sequence.
ACCESSION   DAPHCV010000371 DAPHCV010000000
VERSION     DAPHCV010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.376"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..77)
                     /locus_tag="RH033_005014"
     CDS             complement(<1..77)
                     /locus_tag="RH033_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV2994041.1"
                     /translation="MTETESAILAHARRCAPAESCGFVV"
     gene            complement(83..781)
                     /locus_tag="RH033_005015"
     CDS             complement(83..781)
                     /locus_tag="RH033_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDV2994042.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTVVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            complement(791..>880)
                     /locus_tag="RH033_005016"
     CDS             complement(791..>880)
                     /locus_tag="RH033_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV2994043.1"
                     /translation="PIRVVCRKWSASVGALWVTITADFEQVVA"
BASE COUNT          186 a          282 c          215 g          197 t
ORIGIN      
        1 ctcaccacga agccgcacga ctccgctggc gcacatcgcc gggcgtgcgc cagaatcgct
       61 gattctgtct ctgtcatggg attcactgcg aaagtttgtt aatggaaagg aagccgccaa
      121 agttgccgac gttattgcga aacttacagc cgctcaggca tttgctgcat ttatccttcg
      181 tgatatcgga cgtcggctga tcatattcat ccgcgaccgc cggaccgtga taaccgcact
      241 catcaccgcg ataggtccag gtgcaggtgt tggccagcat gatacgtccc ggaaaaacgg
      301 caccgtccgt ttccgtcggc gtggacagta caaaagaggc actcaccgcg ctcagttcgc
      361 tgcactgctc gatgcgccag cggctgatca cctcctgctc cggatcggcg tcactgtttc
      421 cgttgacgaa gttcaccgca tccagaaaac gggcgtaaac cttacgccgg accaccgttc
      481 ctccgaccag actctgcagg tcttccgcca tcccggtgac catgccgtgc aggttagaaa
      541 ccgtcagtgt cggacgggca gcactgccct tgccgttcag ttcaaatccc gtcccctgaa
      601 tgggatacgc ctgatactgt cgcccctgcc aggtgaccgg ctcacctttt tcgttctgct
      661 cattacagaa aaaataacgt tctccaccga ccactgtcag atcgatttcc cagagcacca
      721 cctgggctga ctgagtgagg cgtgtcgtct catgatgtgt ttcctgtgga atatcctgca
      781 tcagagactc ctatgccacg acctgttcaa aatctgccgt tatggttacc cacaacgccc
      841 ccacgcttgc cgaccattta cgacaaacca ccctgatcgg
//
LOCUS       DAPHCV010000372          878 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.377, whole genome shotgun sequence.
ACCESSION   DAPHCV010000372 DAPHCV010000000
VERSION     DAPHCV010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.377"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>878)
                     /locus_tag="RH033_005017"
     CDS             complement(<1..>878)
                     /locus_tag="RH033_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDV2994044.1"
                     /translation="GKTTQFRAVMSAVNMLPASERPRVVGLGPTHRAVGEMRSAGVDA
                     QTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASG
                     DTDQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINRDVERALSGLE
                     SVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGETFPDVPMTLYEAIVR
                     DYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVPVLNTANIRDG
                     ELRRLSTWENNPDALA"
BASE COUNT          151 a          299 c          240 g          188 t
ORIGIN      
        1 ggcaagggca tccgggttat tctcccaggt ggagagacga cgcagctccc cgtcacgtat
       61 attcgctgtg ttcaggacag gcaccatgac ctgctctttc cccagctcac cggccttttc
      121 ccgtgcatca tgaatcatgc tgttcagtac gcgccggtcc tcattcaggt gcgtgacaat
      181 cagcgtctgc tcccgtgctt ccggtgtcct gccggtatag tcgcggacaa tggcttcata
      241 cagtgtcatg gggacatccg gaaaagtctc acctttcagc atcgccttct gctgcgcttc
      301 tgccagtttc gcttcctggc tgtgactgaa ctccgtcacg gagtgctccg gtgcccatgc
      361 gccctcctga cggggaacct gagacggttt cacactctca agcccggaca gtgccctttc
      421 cacatcccgg ttaatcaggc tgtataccgc ctcccgcagt tccggcgtct gacgcacaat
      481 ctccttcatg atgaccacat cggcagcact gcgcgtctgc tggagacgga aaggctgacc
      541 gggcgcgatg gcctgcagct ggtccgtgtc accactggcc acggcacgac cgccaccggc
      601 cgcaatcagg gcgtatgccc gtgccatgtc ggtattgccc accattgagc tctcatcgag
      661 caggaacagc gtgttgctga aatccggcgt ttctccgctg cgctgctgca gctgcgtgtc
      721 atgcagaaag gacgccagcg tctgtgcatc cacgccggcg ctgcgcatct caccgaccgc
      781 acggtgcgtg ggccccagcc ccacaactcg gggacgctca ctcgccggca gcatgttcac
      841 ggctgacatc accgcccgga actgtgtggt cttaccca
//
LOCUS       DAPHCV010000373          873 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.378, whole genome shotgun sequence.
ACCESSION   DAPHCV010000373 DAPHCV010000000
VERSION     DAPHCV010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.378"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>873
                     /gene="parM/stbA"
                     /locus_tag="RH033_005018"
     CDS             <1..>873
                     /gene="parM/stbA"
                     /locus_tag="RH033_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HDV2994045.1"
                     /translation="STNIKLQWQESDGTIKQHISPNSFKREWAVSFGDKKVFNYTLNG
                     EQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSGLPVSEVDIVCTLPLTEYYDR
                     NNQPNTENIERKKANFRKKIILNGGDTFTIKDVKVMPESIPAGYEALQELDELDSLLI
                     IDLGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTKGSSYLADDII
                     IHRKDNNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHVMVIGGGAE
                     LICDAVKKHTQIRD"
BASE COUNT          304 a          166 c          184 g          219 t
ORIGIN      
        1 gttcaacaaa catcaaacta cagtggcagg aaagcgacgg aacaattaaa cagcacatta
       61 gcccgaacag tttcaaacgc gagtgggcag tctcttttgg tgataaaaag gtctttaact
      121 acacactgaa cggcgaacag tattcatttg atccaatcag cccggatgct gtagtcacaa
      181 ccaatatcgc atggcaatac agcgacgtta atgtcgttgc ggtgcatcac gccttactga
      241 ccagtggtct gccggtaagc gaagtggata ttgtttgcac acttcctctg acagagtatt
      301 acgacaggaa taaccaaccc aatacggaaa atattgagcg taagaaagca aacttccgga
      361 aaaaaattat attaaatggt ggggatacat tcacaataaa agatgtaaaa gtcatgcctg
      421 aatctatacc agcaggttat gaagctctac aagaactgga tgagttagat tctttattaa
      481 ttatagacct cgggggcacc acattagata tttctcaggt aatggggaaa ttatcaggga
      541 tcagtaaaat atacggagac tcatctcttg gtgtatctct ggtcacatct gcagtaaaag
      601 atgcactttc tcttgcgaga acaaaaggaa gtagctatct tgctgacgat ataattattc
      661 acagaaaaga caacaactat ctgaagcaac gaattaatga tgagaacaaa atatcaatag
      721 tcaccgaagc aatgaatgaa gcacttcgta aacttgagca acgtgtatta aatacgctca
      781 atgaattttc tggttatacg catgttatgg ttataggcgg tggcgcagaa ttaatatgcg
      841 atgcagtaaa aaaacacaca cagattcgtg atg
//
LOCUS       DAPHCV010000374          867 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.379, whole genome shotgun sequence.
ACCESSION   DAPHCV010000374 DAPHCV010000000
VERSION     DAPHCV010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.379"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>867
                     /locus_tag="RH033_005019"
     CDS             <1..>867
                     /locus_tag="RH033_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HDV2994046.1"
                     /translation="ATNSTSETLAATPKAVKSAYDNAEKRLQKDQNGADIPDKGRFLN
                     NINAVSKTDFADKRGMRYVRVNAPAGATSGKYYPVVVMRSAGSVSELASRVIITTATR
                     TAGDPMNNCEFNGFVMPGGWTDRGRYAYGMFWQYQNNERAIHSIMMSNKGDDLRSVFY
                     VDGAAFPVFAFIEDGLSISAPGADLVVNDTTYKFGATNPATECIAADVILDFKSGRGF
                     YESHSLIVNDNLSCKKLFATDEIVARGGNQIRMIGGEYGALWRNDGAKTYLLLTNQGD
                     VYGGWNTLRPFAI"
BASE COUNT          228 a          174 c          228 g          237 t
ORIGIN      
        1 gcgaccaaca gtacgtctga aacgctggcg gcaacgccaa aggcagtaaa atcagcctat
       61 gacaatgcag agaaacgtct gcagaaagac cagaacggcg ctgatatacc cgataaggga
      121 cgcttcctga acaacattaa cgcggtcagt aaaacagact ttgctgataa gcgtggtatg
      181 cgttatgtgc gggttaacgc tcctgcaggt gcaacatctg gaaaatatta ccctgttgtt
      241 gttatgcgtt ctgctggctc agtaagcgaa ctggcatcaa gggtcattat caccacggca
      301 acgcgaaccg caggcgatcc gatgaataac tgcgagttta acggatttgt tatgcctggt
      361 ggctggactg acagggggcg ttatgcttat ggaatgttct ggcaatatca aaacaatgaa
      421 cgagccatcc actcaataat gatgagtaat aagggcgatg atttgcgctc tgtgttctat
      481 gttgatggcg ctgctttccc tgtttttgcg tttatcgaag atggcctgtc aatatccgca
      541 cctggtgctg atctcgttgt taatgatacg acctataagt ttggggcaac aaatccggcg
      601 actgaatgta tcgcggcgga cgttatcctt gattttaaga gtgggcgtgg tttttatgag
      661 tctcattcgt taatcgttaa cgataacttg tcgtgcaaaa aactttttgc cacagacgaa
      721 attgtagcgc gtggtggtaa tcagattcga atgataggtg gggagtatgg tgcattatgg
      781 cgtaatgatg gcgctaaaac ttacctgctg cttaccaatc aaggtgatgt ttatggtggc
      841 tggaatacat taagaccgtt tgctatt
//
LOCUS       DAPHCV010000375          852 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.380, whole genome shotgun sequence.
ACCESSION   DAPHCV010000375 DAPHCV010000000
VERSION     DAPHCV010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.380"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>852
                     /locus_tag="RH033_005020"
     CDS             <1..>852
                     /locus_tag="RH033_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDV2994047.1"
                     /translation="SFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFRGMNNTN
                     YNMAVCGTSGAGKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLR
                     FNPFANITDIDQSAERVRDQLSVMASPNGNLDEVHEGLLLQAVRASWLAKENRARIDD
                     VVDFLKNASDSEQYAGSPTIRSRLDEMIVLLDQYTANGTYGQYFNSDEPSLRDDAKMV
                     VLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRNLKKLNVIDEGWRLLDFKNHKVGE
                     FIEKGYR"
BASE COUNT          194 a          219 c          250 g          189 t
ORIGIN      
        1 agagctttaa tgttgccaac ctgatgccgt tagtggcgga taaccccctg acaccggcag
       61 gtctgctggc acccacctac cgtaaccagc tggcgtttat cgatattttc ttccggggga
      121 tgaataacac caactacaac atggcggtct gtggcacctc cggggccggt aaaaccgggc
      181 tgatacagcc acttatccgc agcgtgctgg actccggggg ctttgccgtg gtgttcgaca
      241 tgggggatgg ctacaagtcc ctgtgtgaga acatgggtgg cgtatatctg gacggtgaaa
      301 ccctgcgttt taacccgttt gcgaacatca ccgatattga ccagtcagcg gagcgtgtcc
      361 gtgaccagtt gtcagtgatg gccagcccca acggtaacct ggatgaagtg catgaaggtc
      421 tgctgctgca ggcggtcagg gcctcctggc tggccaaaga gaacagggca cgtattgatg
      481 acgtggtgga tttcctgaaa aacgccagtg acagcgagca gtatgccggg tcaccgacca
      541 tccgcagccg tctggacgaa atgattgtgc tgcttgacca gtacactgcc aacggcactt
      601 acggccagta ttttaactcc gatgagccgt ccctgcggga tgacgcaaaa atggtggtgc
      661 tggagctggg cggactggaa gaccgtccgt cactgctggt tgcggtgatg ttctccctga
      721 ttatctacat cgagaacagg atgtaccgca cgccgcgtaa cctcaagaaa ctgaacgtta
      781 ttgatgaagg ctggcgtctg ctggacttca aaaatcacaa ggtcggtgaa tttattgaga
      841 aaggctaccg ta
//
LOCUS       DAPHCV010000376          847 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.381, whole genome shotgun sequence.
ACCESSION   DAPHCV010000376 DAPHCV010000000
VERSION     DAPHCV010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.381"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..534
                     /locus_tag="RH033_005021"
     CDS             <1..534
                     /locus_tag="RH033_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HDV2994048.1"
                     /translation="YHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSD
                     TPLDDREKGFISSTTVPVFKYLIDPQMLGVSSSVVYQLTDYIGYDIMLQYIQELLQQA
                     RAMIATGNYPQTVLDSVLENLNQAQQQIAVFQGQVKVQQDALLVVDRQMSYMRQQLSA
                     RMLSRYQNNYHFGGSTL"
     gene            531..>847
                     /locus_tag="RH033_005022"
     CDS             531..>847
                     /locus_tag="RH033_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HDV2994049.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVQVHRVDNVPVGLAMPL
                     SLTT"
BASE COUNT          199 a          209 c          236 g          203 t
ORIGIN      
        1 tatcactgca acgattcgga caagtgtctg aaggtggtgg cggatgcaaa tgtgaccatc
       61 gcccgggata aagccctgaa atcacagatt acaaaactgc tcaccagtat tcagaataaa
      121 gcggtgagtg atactccact ggatgacagg gaaaaaggct tcatttccag caccaccgtc
      181 cccgtcttta aatatcttat tgacccgcag atgctcggtg tatccagcag cgtggtttac
      241 cagctgacgg actatatcgg ctacgacatc atgctccagt acattcagga actgcttcag
      301 caggcccggg ccatgattgc caccgggaac tatccacaga ccgtgctgga cagtgtgctg
      361 gagaatctga atcaggcaca gcaacagatt gcggttttcc aggggcaggt aaaggtccag
      421 caggatgcat tgctggttgt tgaccgtcag atgagctata tgcgccagca gctttccgcc
      481 cgcatgctca gccgttacca gaacaactat cacttcggag ggagcacgct gtgaatgaag
      541 tctatgtgat tgccggtggt gagtggttac gcaataacct gaacgccatt gccgccttta
      601 tggggacccg gacgtgggat tccatcgaaa aaattgcgct cacattgtct gttctcgcgg
      661 tggccgtgat gtgggtacag cggcacaacg tgatggattt gctgggctgg gtggccgtgt
      721 ttgtgcttat cagcctgctg gttaatgtcc gtacatcggt gcagattatt gataacagtg
      781 acctggtcca ggttcaccgg gtggataatg tgcctgtcgg actggcgatg ccactttcac
      841 tgacgac
//
LOCUS       DAPHCV010000377          841 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.382, whole genome shotgun sequence.
ACCESSION   DAPHCV010000377 DAPHCV010000000
VERSION     DAPHCV010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.382"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            171..>841
                     /locus_tag="RH033_005023"
     CDS             171..>841
                     /locus_tag="RH033_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin-binding protein"
                     /protein_id="HDV2994050.1"
                     /translation="MLKRRDAFLKKSALAVSVALLLSSQALAHKTLSEPLTGTIWLDN
                     GTQSLESASVIDRNGNANGDTNVTGKNFAVGSGAIIWDAGKSMAVGHKTAVFNADNSV
                     ALGYGSQVNGESNVLSVGAGPSGYGFSVDGAPETRRIINVSDGVKDSDAATKGQMDNA
                     IADAVRVSGDALRGEIGGVYRDARAHTDSQVTAVRDELKAEGDSLRGEIGGVYRDARA
                     HTDSQV"
BASE COUNT          224 a          150 c          231 g          236 t
ORIGIN      
        1 ttttaatgat tttcgcgtca ttgcttttct gctgcttaaa ttgaggtcgc atattataca
       61 caataatatt cctggcatta ttaagttaat aagagaggat tcttatcact gtattttttc
      121 tccgcactac cgttctggct ggtgaagtaa taatacagga gaataacaga atgcttaaaa
      181 gacgtgatgc ttttttaaag aaaagcgcgc tggcagtgag tgtggccctg ctgctctcat
      241 ctcaggcttt ggctcataaa acactttctg agccattgac gggtactatc tggcttgata
      301 atggcactca gtcacttgaa agtgcaagtg tgattgacag aaatggaaat gctaacggtg
      361 atactaatgt tactggaaaa aactttgctg ttggttccgg tgcaataatt tgggatgcgg
      421 gtaaatccat ggcagtggga cataagacag ctgtttttaa tgcagacaac agtgtcgctc
      481 tggggtatgg ctctcaggtg aatggagaaa gcaatgtact ttctgtaggg gccggtcctt
      541 caggatatgg attttcagtt gatggtgcac cggaaacccg ccggattata aatgtttcag
      601 atggtgttaa ggatagtgat gcggccacaa aaggacagat ggacaacgcc attgcagatg
      661 ctgtacgggt gtcgggggat gccctgcgcg gtgagatagg tggtgtatac agagatgccc
      721 gtgctcatac tgacagtcag gttacagcag tacgtgatga actgaaggca gagggagaca
      781 gtctgcgtgg tgagataggt ggtgtataca gagatgcccg tgctcatact gacagtcagg
      841 t
//
LOCUS       DAPHCV010000378          834 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.383, whole genome shotgun sequence.
ACCESSION   DAPHCV010000378 DAPHCV010000000
VERSION     DAPHCV010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.383"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..376)
                     /locus_tag="RH033_005024"
     CDS             complement(<1..376)
                     /locus_tag="RH033_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HDV2994051.1"
                     /translation="MPFSNSSLSAQVKSYLTFLPEEIRQKILEHLHGVIHYEPVIGIM
                     GKSGTGKSSLCNAIFQSRICATHPLNGCTRQAHRLTLQLGERRMTLVDLPGIGETPQH
                     DQEYRALYRQLLPELDLIIWILR"
     gene            477..>834
                     /locus_tag="RH033_005025"
     CDS             477..>834
                     /locus_tag="RH033_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001702852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2994052.1"
                     /translation="MAKNKGICRNKKCSNPVSEFDEANALRLCDDHYKLWKRNKENRR
                     VRNICRIISKYDKSLDREIFYGVRDAAKNKTGKRIIEDAKILHQCRASKQVRKSFHKG
                     VLRSREWLNTQELLSRK"
BASE COUNT          258 a          153 c          218 g          205 t
ORIGIN      
        1 tccgcaggat ccagataatc agatccagtt ccggcagtaa ctgacgataa agcgctcggt
       61 attcctgatc atgctgcggt gtttcaccaa tgccgggcag atcgaccagc gtcattctgc
      121 gttcaccgag ctgcagggta agacgatgag cctggcgggt gcagccgttc aggggatgcg
      181 tggcgcagat acgggactga aaaatggcat tacacaggct gctcttgccg gtgccggatt
      241 tacccataat gccaatcacg ggctcgtaat gaataacacc gtggagatgt tcaaggattt
      301 tctggcgtat ttcttcagga agaaacgtca gatatgattt cacttgcgcg gagagggaag
      361 aattgctgaa tggcataccg gtatcctgtt gatttcactg aaccggatca cactaatccg
      421 gtttatcatt caggtaatat gggtgtgaaa atttttccat tgacgggaag aagagtatgg
      481 ctaaaaacaa aggtatttgt cggaataaaa aatgcagtaa tcctgtttca gaatttgatg
      541 aggcaaatgc cttaaggtta tgcgatgatc attataagct atggaagcga aataaagaga
      601 atcgtcgggt caggaatatt tgtcgtataa tcagtaaata tgacaaaagt ctggaccggg
      661 agatatttta tggagtaaga gatgctgcaa aaaataaaac gggaaagaga attattgagg
      721 acgcaaaaat attacatcaa tgccgtgcct caaagcaagt aagaaaaagt tttcacaagg
      781 gtgtgcttcg gtcacgggaa tggcttaata cacaggagtt actgagcaga aaac
//
LOCUS       DAPHCV010000379          820 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.384, whole genome shotgun sequence.
ACCESSION   DAPHCV010000379 DAPHCV010000000
VERSION     DAPHCV010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.384"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..131)
                     /locus_tag="RH033_005026"
     CDS             complement(<1..131)
                     /locus_tag="RH033_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV2994053.1"
                     /translation="MSDNRSRHDRLAVRLSLIISRLMAGESLSLKTLSDEFGVTERT"
     gene            complement(334..579)
                     /locus_tag="RH033_005027"
     CDS             complement(334..579)
                     /locus_tag="RH033_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase"
                     /protein_id="HDV2994054.1"
                     /translation="MSLMIHALPPQATSAVYSHIRGENRSEQARKHAQQTFGDAIGKS
                     FDDAVARFSFPAWMLQLLRKARDRIIHLLVTLWDRLF"
BASE COUNT          222 a          183 c          218 g          197 t
ORIGIN      
        1 aaggtacgtt ctgtaacacc aaattcatct gacagtgttt ttagtgacag agattctccg
       61 gccatcagtc ggctgataat gagtgataag cgaaccgcca ggcgatcatg ccgggaacga
      121 ttatcagaca tagatgtgca ctccgtaaga aaacatcttt tatttcatga tgttattatg
      181 catcatgatg ttgaaaggat gtgtcgcatc cgaataatca gaaataaaat gcagcagaaa
      241 aaatattcct ttaattatct gctttttaag cagggtttca taaaaagaag aatgcttgtt
      301 aatccagcga cacatcctgt cggcgtgagt gtgtcagaac agacgatccc acagtgtgac
      361 cagcaagtgg ataatgcggt cccgggcttt acgcagaagc tgtaacatcc aggccggaaa
      421 actgaaccgg gcaacggcgt cgtcaaaact tttcccgatg gcatcaccaa aagtctgttg
      481 tgcgtgtttc cgggcctgtt cagagcggtt ttcccccctg atatgtgaat aaactgcgct
      541 ggttgcctgt ggtggcagcg cgtggatcat cagtgacacc agcgctggaa ggttccagcc
      601 tgcaggcgcg gctacaggga gtaccggaaa tgatgaaggg aacagggtgg ccacccgggc
      661 tgttactgtc gccagtgaga gttcctggtg acgggacggg catttttctg tggcattcca
      721 ttcttcagca ggaaacatgc gatcggcatg gctgagaaca aacagaaagc gcgagggatc
      781 tgcgccctca ttcagtaaaa actgatgcat ggcaatatcg
//
LOCUS       DAPHCV010000380          816 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.385, whole genome shotgun sequence.
ACCESSION   DAPHCV010000380 DAPHCV010000000
VERSION     DAPHCV010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.385"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(190..720)
                     /locus_tag="RH033_005028"
     CDS             complement(190..720)
                     /locus_tag="RH033_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HDV2994055.1"
                     /translation="MSLRRMTSGELTLARSIFENTIDYSKVWIHNEKYLPGQGERTAM
                     TPNGEMYYPDAVYSSDYSSQAMPGDRETVAGASHLFIHEMMHVWQYQKGYAVKLRGLF
                     SWAANYHYDLNLQSISSYSMEQQASIVADYWLLINYGLVADLANVNYVGDTTESVNTL
                     SEKYKKVLNLFPAGVI"
BASE COUNT          268 a          183 c          113 g          252 t
ORIGIN      
        1 ctgtcactct tttcaacttt attgcattgc catatttacc ttcatataaa acataatttg
       61 aaataatatt cccatcgtct ggttttgaac aaatctgtcc gtcttgcaaa tatacactac
      121 cgggtaacaa tgggcgaaca taatcaccaa cagtgcatcc cgacaaaaag aaaaacaaaa
      181 agaatctctt catatgacac ccgcaggaaa taaattaagc acctttttgt atttttccga
      241 tagagtgttc actgattcag ttgtatcacc tacatagtta acatttgcca aatcagccac
      301 cagtccatag ttaattaata gccaataatc agcaactata gatgcttgtt gttccatgga
      361 ataagaagaa attgactgta aattaagatc gtaatgataa tttgccgccc aagaaaataa
      421 accacgcaac ttaactgcat accctttctg atattgccaa acatgcatca tttcatgaat
      481 aaacaagtga gaagcacctg ctactgtttc acgatcacca ggcattgctt gcgatgaata
      541 atctgaacta taaacagcat caggataata catttccccg tttggggtca tcgctgttct
      601 ttccccttga ccaggcaaat atttttcatt gtggatccat accttgctat aatcaattgt
      661 attctcaaaa atcgatctgg ccaatgttaa ttcaccagat gtcattctcc ttaatgacat
      721 atcctttccc ctggaaataa tatctttcag ataaaacaca aacgcccttt gatgttcaat
      781 attcccactt tatacagtat tgatgacatc atcttc
//
LOCUS       DAPHCV010000381          816 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.386, whole genome shotgun sequence.
ACCESSION   DAPHCV010000381 DAPHCV010000000
VERSION     DAPHCV010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.386"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..737
                     /gene="ydeO"
                     /locus_tag="RH033_005029"
     CDS             <1..737
                     /gene="ydeO"
                     /locus_tag="RH033_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HDV2994056.1"
                     /translation="IHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPYERHFKEIGF
                     NENTIKKYLQCTNIQTVTMPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLL
                     FSCLSIFATCKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKK
                     KLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSP
                     KRVSKEYRCQRHTGMNTDNTMSALAI"
BASE COUNT          250 a          166 c          163 g          237 t
ORIGIN      
        1 ttattcatca tgccttcaac gctaacattt tagataaaga ttacgccttc tctgacggcg
       61 agatcctgat ggtagataac gctgttcgta cgcattttga accttatgag cggcatttca
      121 aagaaatcgg atttaatgaa aacaccatta aaaaatatct acaatgcact aacatccaga
      181 cagtgacgat gcctgttcct gcgaagtttt tacgtgcttc aaatgtaccg actggattgc
      241 ttaatgaaat gattgcttat ctcaactcgg aagaacgcaa tcatcataac ttttcagaac
      301 ttttgctttt ttcttgcctg tctatttttg ccacatgcaa aggtttcatt acactattaa
      361 ctaacggtgt gctatccgtt tccgggaaag tgagaaatat tgtcaacatg aagctggcgc
      421 acccatggaa gctgaaagat atttgtgact gcctgtacat tagtgaaagc ctgttgaaga
      481 aaaaactaaa gcaagagcaa acgacattct cacagattct tttagatgcc agaatgcagc
      541 acgcaaaaaa tttgatacgc gtagaaggtt cagtcaataa aattgccgaa caatgtggtt
      601 atgcgagtac atcttatttt atttatgcgt tccgcaaaca tttcggcaac agtccgaaga
      661 gagtttctaa ggagtaccgt tgtcaaaggc acacgggtat gaacacggat aacacgatga
      721 gtgctttagc tatttgatta tttgctaacg agtagtcaac cacgcacgtt acgtagaaat
      781 taaaatgggg cagccattcc ctgccccgcg ttgttt
//
LOCUS       DAPHCV010000382          808 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.387, whole genome shotgun sequence.
ACCESSION   DAPHCV010000382 DAPHCV010000000
VERSION     DAPHCV010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.387"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>808
                     /locus_tag="RH033_005030"
     CDS             <1..>808
                     /locus_tag="RH033_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV2994057.1"
                     /translation="KSKNTEISPDYLWDDGVNGLLINYYAYGNYYLKNTYKMSNSQYA
                     NFEPQLNIDAWRIKNQFVWSKEDNHNRLTSNYLYAERGLNSIKSRIYIGSGYFPLRGF
                     DSFKFQGGVLKTDESMYPSIEKKFSPVVKGIAKSQSKVEIYQDKTMIYTTTVPAGPFE
                     ITDYLLSGSNSDLYVKVIDTYGNTQTFIVPYSLPAIAVRNGYRYYEIAAGNYQELDED
                     FANFYSIIGLPYDFTVYGGAELSSKYNSINMGLGKIIGDLGAVSYAVTASK"
BASE COUNT          279 a          126 c          152 g          251 t
ORIGIN      
        1 aaatcaaaaa atacagagat ttcgcctgat tatttatggg atgatggcgt taatgggctt
       61 cttataaact attatgcata cgggaactat tacttaaaaa acacatacaa aatgagcaac
      121 tcgcaatatg ctaattttga accccaatta aacatcgatg cctggcgaat aaagaatcag
      181 ttcgtatggt caaaagagga taatcacaac agattaacaa gtaattacct gtatgctgag
      241 agaggtctaa atagcatcaa aagccgtatt tatattggtt ctggatactt tcctttaaga
      301 ggatttgatt cgttcaagtt tcaaggtgga gtattgaaga cagacgaatc aatgtatccg
      361 tcaattgaga aaaaattttc tcctgttgta aaaggaatag caaaaagtca gtctaaggtg
      421 gaaatttatc aagataaaac aatgatatat acaacgacag tgcccgctgg tccttttgaa
      481 ataactgatt atttgttatc aggctcaaat tctgacttat atgtcaaagt tattgatact
      541 tatggaaata cacaaacttt catagtgcct tattctctac cggcaatagc agttaggaat
      601 ggatatagat actatgaaat tgctgcaggt aattaccaag agttagatga agattttgca
      661 aacttctatt ccatcattgg tctaccatat gattttactg tctatggggg agccgaactt
      721 tcatccaaat acaattctat aaacatgggg ttgggtaaga ttattggtga tttaggtgca
      781 gtttcttatg ctgttactgc atcaaaaa
//
LOCUS       DAPHCV010000383          808 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.388, whole genome shotgun sequence.
ACCESSION   DAPHCV010000383 DAPHCV010000000
VERSION     DAPHCV010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.388"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..792)
                     /locus_tag="RH033_005031"
     CDS             complement(<1..792)
                     /locus_tag="RH033_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDV2994058.1"
                     /translation="MAILDDVIGVFSPGWKAARLRSRAVIQAYEAVKTTRTHKARREN
                     RTADQLSQYGAVSLREQARYLDNNHDLVIGVFDKLEERVVGKNGIIVEPHPVLRNGAI
                     ARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLMLRTWLRDGEVFAQMVSGRINSLTP
                     SAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDDWGRPEKYLVYKSRPVSGRQMETKE
                     VDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSALKEYEDSELTAAR"
BASE COUNT          186 a          255 c          189 g          178 t
ORIGIN      
        1 ccttgctgca gtcagctcag aatcttcata ctctttcagg gcgctgaggc ggatcagcac
       61 accggacaat aaagacgtcc cgcgcatctg gtgcagacgg cgaacaaatt taagatgcag
      121 cattcgctct gcatccactt ctttggtttc catctgccgt ccggatacag gacgactttt
      181 atacaccaga tatttttcgg gacgccccca gtcatcaaca aacacgccct gattcagcct
      241 gttgctctca tcactggtca tgggaataaa gtccggctcg agcgcctcca gccagaaatg
      301 aacaccggca gaaggcgtca ggctgtttat gcgcccggaa accatctggg caaacacctc
      361 accatcgcgc agccaggtac gcagcatcag acgttccagc atcggacggg taaactgccc
      421 ggtgacctcc ggactgacag accattcact ccatcgggta cgtatctccg cagccaggtc
      481 acgggcaatg gccccattgc gtaataccgg atgtggctcg acaataatcc cgtttttccc
      541 caccacccgt tcttccagct tgtcaaatac accaatgacc agatcgtggt tgttatcaag
      601 gtaacgggcc tgctcacgta acgacacggc cccgtactgg cttaactggt cggcagttcg
      661 gttctcccgt cgggctttgt gtgtccgcgt cgtttttacg gcctcataag cctggatcac
      721 cgcacgggaa cgcagccttg ccgctttcca tcctggtgaa aaaacgccaa tcacatcatc
      781 aagaattgcc atcagaacct cgccagcc
//
LOCUS       DAPHCV010000384          790 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.389, whole genome shotgun sequence.
ACCESSION   DAPHCV010000384 DAPHCV010000000
VERSION     DAPHCV010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.389"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            22..345
                     /locus_tag="RH033_005032"
     CDS             22..345
                     /locus_tag="RH033_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2994059.1"
                     /translation="MGRNMSAARTDGFIRNIHSRNPFDVIRADVVISRLEKQAHWGCG
                     LHYEIYEANLFDMAMNHLSRLPLKDRPVFIGAAARKGLFLTMEETRLAREAKDALMAE
                     LALEY"
     gene            404..646
                     /locus_tag="RH033_005033"
     CDS             404..646
                     /locus_tag="RH033_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2994060.1"
                     /translation="MGYITYVTDQRPGEPDILTGNTFADLEICDSDGHLLLKVSAPEA
                     GWTHESLNLVQPQEVQEGNDAFDAYLNGIWIGSTEV"
BASE COUNT          198 a          163 c          228 g          201 t
ORIGIN      
        1 aaaaacagag gtgtattaaa gatgggcaga aatatgtcag cagcacgtac cgatggcttt
       61 attcgtaata ttcattcccg caatcccttt gatgttatcc gggctgatgt ggtgatcagc
      121 aggctggaaa agcaggctca ttggggatgt ggtctgcact atgaaattta tgaagcgaat
      181 ttgtttgata tggcgatgaa tcatctttcc cggcttcctc tgaaggatcg tccggtattc
      241 attggtgctg ctgcacgaaa ggggcttttt ctgacgatgg aagaaaccag gctggcacgg
      301 gaagcgaaag atgctctgat ggctgaactt gctctggaat actgaaaatc gggccgcacc
      361 gtcgtccagg ataatgctga aatatttaat gtggagagaa tggatgggat atatcacata
      421 tgttacagat caacgacccg gtgaaccaga tatcctgacc gggaacacgt ttgctgatct
      481 ggagatttgt gattctgatg ggcatcttct gctgaaagtg tctgcacctg aagccggctg
      541 gacacacgaa agcctgaacc ttgttcagcc gcaggaagtg caggagggga atgatgcatt
      601 tgatgcttat ctgaatggta tatggatcgg ttcgacagag gtctgaacaa atcacgccag
      661 cagaagacgt tgctggcgtc ttctgcattc ccggggagct gtcgcgcggc atgtttgctt
      721 atacccgtgc cccgagatga ttatttgtct gcgggcctga agacgccggg caggcgggag
      781 gataaatgcc
//
LOCUS       DAPHCV010000385          787 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.390, whole genome shotgun sequence.
ACCESSION   DAPHCV010000385 DAPHCV010000000
VERSION     DAPHCV010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.390"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
BASE COUNT          262 a          157 c          160 g          208 t
ORIGIN      
        1 acgcttacca acagtctacc agacacttgt gcgagggaaa aatgattgat ttgatagcag
       61 aacagcgaca actggcaaag atagttcatg attatgccag tcggttccca cagacagcat
      121 atggcgatgc tcaattactg caaggttgtt atgactacat ggatgccttt aagcgggtga
      181 tggacagttc ctcaaaaaat tcagatggat tacatctgtc tgcaataccc cgggtatttt
      241 cggtttgcga aatggatgga acaacttgct cgggggatag ctgatggagt tattgaagtg
      301 ccgaaggccc cctaaaaatc acagccggaa agttcgacgg ttccggcgta gaccacctgg
      361 tactgttttt gtcatgtatc acctatgact gagaatttac tcacttagtc acgtatccat
      421 tattgcttgg taaggtcaca tcaggcaaat caaaacagaa gtagtggata ttttcttgtc
      481 taaaaatcca tcgccgcatc tcacaatacc gaaaggtacg attattaccc gacaaaacta
      541 ttattcaaaa tacacctgtg gaatgtttac caaaaaatag tacccccgag gcgctcaaag
      601 ttcgcatttt ggtaaaaatt tatctagttg caatgcaata acaaaacaga atagaactca
      661 aaattgctat ttcaggagaa ctcaaaatga gaaaggttga tgcttttgaa agctcagatg
      721 agaacaatac ttgcaatagt ttattaggca taaaaataaa aaacattgtc acacaaaaac
      781 acctgtt
//
LOCUS       DAPHCV010000386          786 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.391, whole genome shotgun sequence.
ACCESSION   DAPHCV010000386 DAPHCV010000000
VERSION     DAPHCV010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.391"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            51..767
                     /locus_tag="RH033_005034"
     CDS             51..767
                     /locus_tag="RH033_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV2994061.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          263 a          134 c          167 g          222 t
ORIGIN      
        1 tgttaacgat ttaccagtaa tgtaaataaa ttttcgagga gatcattcca gtgggacgta
       61 aatgggccaa tattgttgct aaaaaaacgg ctaaagacgg tgcaacgtct aaaatttatg
      121 caaaattcgg tgtagaaatc tatgctgctg ctaaacaagg tgaacccgat ccagaattaa
      181 acacatcttt aaaattcgtt attgaacgtg caaagcaggc acaagttcca aaacacgtta
      241 ttgataaagc aattgataaa gccaaaggcg gcggagatga aacgttcgtg cagggacgtt
      301 atgaaggctt tggtcctaat ggctcaatga ttatcgccga gacattgact tcaaatgtta
      361 accgtacgat tgctaacgtt cgcacaattt tcaataaaaa aggcggcaat atcggagcgg
      421 caggttctgt cagctatatg tttgacaata cgggtgtgat tgtatttaaa ggaacagacc
      481 ctgaccatat ttttgaaatt ttacttgaag ctgaagttga tgttcgtgat gtgactgaag
      541 aagaaggtaa cattgttatt tatactgaac ctactgacct tcataaagga atcgcggctc
      601 taaaagcagc tggaatcact gagttctcaa caacagaatt agaaatgatt gctcaatctg
      661 aagttgagct ttccccagaa gatttagaaa tctttgaagg gcttgttgat gcccttgaag
      721 atgacgacga tgttcaaaaa gtttatcata acgtcgcaaa tctctaatta tattttaaag
      781 aaatct
//
LOCUS       DAPHCV010000387          779 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.393, whole genome shotgun sequence.
ACCESSION   DAPHCV010000387 DAPHCV010000000
VERSION     DAPHCV010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.393"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(3..>779)
                     /locus_tag="RH033_005035"
     CDS             complement(3..>779)
                     /locus_tag="RH033_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDV2994062.1"
                     /translation="FGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAP
                     FVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQI
                     GTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICT
                     GRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCR
                     RGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
BASE COUNT          178 a          214 c          226 g          161 t
ORIGIN      
        1 tatcaggtat gtttaaagct gttctgctgg gcaataccct gcagtttcgg gtggtcgctg
       61 agatatttca gggaggcttt gtagtcttcc agcaacagtt cagcaaagtc catttcgaag
      121 ccgcgacgac acataatgcg catcaccacg atgtcagtgg cttcaccgcc gagagtgaag
      181 gccggaacct gccagccgcg cagacgcaga cgttcagaga ggtcgtagag ggtgtatcct
      241 ggatcttcac catctttcag tttgaagcaa accgccggga tgccttcgtc cgggcgaccg
      301 gtacagatga actcatacgg ccccagtttg gcgatttcat ccgccagata agcggcaacc
      361 tggtaggaag cgttctgtac tttggtatag ccttcacgac cgaggcgcag gaattcatag
      421 tactgtgcaa tcacctgacc cgccgggcgg gagaagttga tggcgaaagt accaatctga
      481 ccgccgaggt agtcaacgtt gaacaccagt tcctgcggca gcgcttcttc atcacgccag
      541 ataacccagc cgcagcccag cggagccaga ccgaatttat ggcctgaagc actgatcgat
      601 ttcacacgcg gcaggcggaa gtcccagacg atatccgggg cgacgaacgg tgccaggaag
      661 ccaccgctgg cggcgtcgat gtgcatgtcg atgtcgatac cggtgtcggc ctggaattta
      721 tccagcgcat cgtgcagcgg ctgcgggaac tcatagttac cggtgtaggt cacgccgaa
//
LOCUS       DAPHCV010000388          773 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.394, whole genome shotgun sequence.
ACCESSION   DAPHCV010000388 DAPHCV010000000
VERSION     DAPHCV010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.394"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            17..712
                     /locus_tag="RH033_005036"
     CDS             17..712
                     /locus_tag="RH033_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDV2994063.1"
                     /translation="MLSCLSPTLVCAEQPHGFIDYRHEYLDDTRTHADRVEFGTFFSN
                     GIGLMGELRYNTDEGDKDKWDPSQFGNNGTGLSVVYRFKPLDDKKFWLEPMFWLDTTQ
                     YWSTYEYGLSAGYDFSKEWKVSGRFRYDMDKATDKSKGYGNDDRNNRRYDVWIDYRPQ
                     KTNFQYQLNLVYYNNGYLTWNDGHKNYTASFKVGYKMGSWIPYMSIADYKGVDKTSSN
                     RQIRWRWGLTYTF"
BASE COUNT          239 a          148 c          164 g          222 t
ORIGIN      
        1 acttgcagga ataacaatgt tatcctgcct gtcacctaca ctagtctgtg cagaacagcc
       61 gcatggattt attgattatc gccatgagta tcttgatgat actcgcaccc acgcggaccg
      121 cgttgaattt ggtacatttt ttagtaatgg tatcggtttg atgggagaat tacgctataa
      181 caccgatgaa ggtgataaag ataagtggga cccctcacaa tttggcaata acggaacagg
      241 attatctgtt gtttaccgct ttaagccgct ggatgacaaa aaattctggt tagaacccat
      301 gttctggctt gataccacgc aatattggtc aacttacgaa tatggtcttt ctgcgggtta
      361 tgacttcagc aaagaatgga aagtttcagg tcggtttcgt tatgacatgg ataaagccac
      421 cgataaatcc aaaggttatg gcaacgatga tcgtaataac cgccgttacg atgtgtggat
      481 cgactatcga ccacaaaaaa ccaacttcca atatcagtta aacctggtct attacaataa
      541 tggttattta acctggaatg atggccataa aaactatacc gcatcattta aagtagggta
      601 caaaatggga tcgtggatac cctatatgag tattgcggat tataaaggtg tggataaaac
      661 atcaagtaat cgccaaatcc gctggcgctg gggtctgact tatactttct aatctggaat
      721 aaaaaatgaa taataaattt aaaatcattc tctggatgat gctctctgct ctg
//
LOCUS       DAPHCV010000389          765 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.395, whole genome shotgun sequence.
ACCESSION   DAPHCV010000389 DAPHCV010000000
VERSION     DAPHCV010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.395"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            44..634
                     /locus_tag="RH033_005037"
     CDS             44..634
                     /locus_tag="RH033_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDV2994064.1"
                     /translation="MSRIYAYCRVSTLEQTTKNQRREIESAGFNIKSQQIIEEQISGS
                     AATSERPGFNQLLDRLKRGDALIVTKLDRLGCNPMDISKTVEQLTKAGIKVHCLALGG
                     VDLTSPPGKMMMQVISAVAEFERDLLLERTHSGILRARGAGKRFGRPPVLNDEQIQRV
                     LEQIKSGINISDIAQEFKTSRQTILRAKAKNQTPDI"
BASE COUNT          251 a          153 c          164 g          197 t
ORIGIN      
        1 tgcattatga tatgtacacc aatttcgtat agaagatctc actatgtctc gaatttatgc
       61 ttactgtcgg gtatcaactc tggaacagac aaccaaaaat caacgtcggg aaatcgaaag
      121 tgcaggtttt aacatcaaat ctcagcaaat aattgaagaa caaattagcg gctcagcagc
      181 aaccagtgag cgtcctggtt ttaaccagtt gcttgatcga ctgaaaagag gtgatgcatt
      241 gattgtcaca aaactggatc gcctcggttg taatccaatg gatatcagta aaacggtgga
      301 acaactgacc aaagcaggta tcaaagtgca ttgcttagca ttggggggcg ttgacctgac
      361 cagtccacct ggaaaaatga tgatgcaagt aatttcagca gtcgctgaat ttgagcgaga
      421 ccttttactt gagcgtactc attcagggat attaagagca cggggcgcag gaaagcgatt
      481 tggtcgccca cctgtgttaa atgatgaaca aatacagagg gtactagagc aaattaagtc
      541 aggtataaat ataagtgaca ttgcccaaga attcaaaacc tcgcggcaaa ccattttaag
      601 agctaaagca aaaaatcaga cgcctgatat ataaaaataa tctcggtgtg agattcctta
      661 cgttttccaa gccccccttc ctagccttaa atggaaagat acttctaatt atagaattta
      721 tatatcttac catacggtag ggctggccat tcaatatccg gtgca
//
LOCUS       DAPHCV010000390          760 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.396, whole genome shotgun sequence.
ACCESSION   DAPHCV010000390 DAPHCV010000000
VERSION     DAPHCV010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.396"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..349)
                     /locus_tag="RH033_005038"
     CDS             complement(<1..349)
                     /locus_tag="RH033_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HDV2994065.1"
                     /translation="MKPNSTGIAARMMLSIDRARISEALLNNRQLQSTPLQIRYPQGV
                     REALGIMSEQLSLSVSDLTRILVEDALTEMFLPAENIVRRLLCRTEHIMQAHDVCVTT
                     MTTLLAPWNIRPAV"
     gene            609..>760
                     /locus_tag="RH033_005039"
     CDS             609..>760
                     /locus_tag="RH033_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2994066.1"
                     /translation="MKIKQHKEKLEIIRDIIRETLLGNAALIVLAAMVPVVLVKPWDS
                     MTFIFMG"
BASE COUNT          190 a          141 c          213 g          216 t
ORIGIN      
        1 agacggcagg acggatattc cagggagcca gtaacgttgt catcgttgtt acgcagacat
       61 catgtgcctg catgatgtgt tctgtccggc agagaagacg gcgaacgatg ttttcagccg
      121 ggagaaacat ttcagttaat gcatcttcga ccaggatacg cgtcaggtcg gatacagaaa
      181 gggagagttg ctcgctcatt attccgagtg cttctctgac gccctgcgga taacggatct
      241 gcaggggagt ggactgcaac tggcggttat tgagcagagc ttcgctaatc cgggccctgt
      301 caatggagag catcatgcgg gcagcaatgc ctgtgctgtt gggtttcatt tcacctccgt
      361 gcggttcata tgttaagtct aaagagcgtg tttactgaac ggaaacgagt tatttcgatc
      421 gttatgatcg agtgtgtttc aagagtgctt ttaactctgt tttaagcgtg aatgaaaagg
      481 attcggacag gacgtgattt agcatgccgc tggtctggca ttatgctgca gagcagacgg
      541 agtcaggaga ttgaaagtat ttcgttatat aatcttttcc ttttcgggat gaaatcaacg
      601 aggttcgtat gaaaattaaa cagcataaag aaaaactgga gattataagg gatattattc
      661 gagagacgct gttgggtaat gctgctctta ttgttctggc agccatggtt ccggttgtac
      721 tggtgaaacc atgggattcg atgacattta tttttatggg
//
LOCUS       DAPHCV010000391          758 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.397, whole genome shotgun sequence.
ACCESSION   DAPHCV010000391 DAPHCV010000000
VERSION     DAPHCV010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.397"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            79..216
                     /locus_tag="RH033_005040"
     CDS             79..216
                     /locus_tag="RH033_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HDV2994067.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAV
                     Q"
BASE COUNT          194 a          176 c          197 g          191 t
ORIGIN      
        1 aaatattttt tatgtagtat cttaagctca taaattaaac ctcgccatat attcttttca
       61 ttttataagg atcgagttat gaggaaaaga ttttttgtgg gaatattcgc gataaacctc
      121 cttgttggat gtcaggctaa ctatatacgt gatgttcagg gagggaccat cgcaccatcc
      181 tcctcttcta aactgacggg gatcgcggtt cagtagaaaa gattaaagga tcttcttgag
      241 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccaacgg
      301 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
      361 gagcgcagat accaaatact gtccttctag tgtagccgta gtcgggccac tacttcaaga
      421 actctgtagc accgtttgtg ccatcatcgc tctgctaatc cggttaccag tggctgctgc
      481 cagtggcgtt aaggcgtgcc ttaccgggtt ggactcaaga cgatagttac cggataaggc
      541 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
      601 caccgaactg agataccaac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
      661 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
      721 tccaggggga aacgcctggt atctttatag tcctgtcg
//
LOCUS       DAPHCV010000392          757 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.398, whole genome shotgun sequence.
ACCESSION   DAPHCV010000392 DAPHCV010000000
VERSION     DAPHCV010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.398"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..486
                     /locus_tag="RH033_005041"
     CDS             <1..486
                     /locus_tag="RH033_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2994068.1"
                     /translation="IYLKMNPQHKLSKRISSAFVASLMILLVISTQITVLPVIFTHSV
                     IKLSGISDFKIHSYIIKTSEYPEEFFSNAVWGKKNIKPGEYYSVQAVSMFTTNQFILL
                     CPKDIIRFYRESWKFDLLNVDFDTNTRKKLQEEAAYCVPISAISVKRWDMPLQGSKPS
                     N"
BASE COUNT          246 a          143 c          147 g          221 t
ORIGIN      
        1 atttatctaa aaatgaatcc acagcataaa ctttctaaaa gaattagttc tgcatttgtc
       61 gcatccttga tgatattatt agttatatcg actcaaataa ccgtattacc agtaattttt
      121 acccattcag ttattaagct ctcaggtata agtgacttca aaatacatag ctacattatt
      181 aaaacaagtg aatatccgga ggagtttttt tccaatgcag tatggggtaa aaaaaatata
      241 aaaccggggg aatattactc tgtgcaggct gtttctatgt ttacgacaaa ccagtttatt
      301 ctcctttgtc ccaaggatat tataaggttt tatcgggaaa gctggaagtt cgacctgctt
      361 aatgtagact tcgatacaaa taccaggaag aaactccagg aagaagcagc atattgcgta
      421 ccaatttctg caatatctgt aaaacgatgg gatatgcctc ttcagggctc gaaaccctca
      481 aattagaagg ccgctcgtgc gactgtatta gatagtaatg cggtatggaa aaatagtgat
      541 agcaagatga acttgacttg gctaatcaaa taccggcgta gtgattagga aacagggctc
      601 ggataacatt attgcgcggt tcattgaacc tactgatgac gccggatata tagtcgtaaa
      661 ccgtttcgaa ccagtactta tatcaccagg agagtccata tatgaccacc gtttacagta
      721 aaaacggtat ctgttttctg atccctccag cgtcaaa
//
LOCUS       DAPHCV010000393          751 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.399, whole genome shotgun sequence.
ACCESSION   DAPHCV010000393 DAPHCV010000000
VERSION     DAPHCV010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.399"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>751
                     /locus_tag="RH033_005042"
     rRNA            <1..>751
                     /locus_tag="RH033_005042"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          189 a          170 c          248 g          144 t
ORIGIN      
        1 gtgcccagat gggattagct agtaggtggg gtaacggctc acctaggcga cgatccctag
       61 ctggtctgag aggatgacca gccacactgg aactgagaca cggtccagac tcctacggga
      121 ggcagcagtg gggaatattg cacaatgggc gcaagcctga tgcagccatg ccgcgtgtat
      181 gaagaaggcc ttcgggttgt aaagtacttt cagcggggag gaagggagta aagttaatac
      241 ctttactcat tgacgttacc cgcagaagaa gcaccggcta actccgtgcc agcagccgcg
      301 gtaatacgga gggtgcaagc gttaatcgga attactgggc gtaaagcgca cgcaggcggt
      361 ttgttaagtc agatgtgaaa tccccgggct caacctggga actgcatctg atactggcaa
      421 gcttgagtct cgtagagggg ggtagaattc caggtgtagc ggtgaaatgc gtagagatct
      481 ggaggaatac cggtggcgaa ggcggccccc tggacgaaga ctgacgctca ggtgcgaaag
      541 cgtggggagc aaacaggatt agataccctg gtagtccacg ccgtaaacga tgtcgacttg
      601 gaggttgtgc ccttgaggcg tggcttccgg agctaacgcg ttaagtcgac cgcctgggga
      661 gtacggccgc aaggttaaaa ctcaaatgaa ttgacggggg cccgcacaag cggtggagca
      721 tgtggtttaa ttcgatgcaa cgcgaagaac c
//
LOCUS       DAPHCV010000394          751 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.400, whole genome shotgun sequence.
ACCESSION   DAPHCV010000394 DAPHCV010000000
VERSION     DAPHCV010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.400"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            93..455
                     /locus_tag="RH033_005043"
     CDS             93..455
                     /locus_tag="RH033_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HDV2994069.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT          242 a          174 c          154 g          181 t
ORIGIN      
        1 ttatagaact attacaatgc catcagttgt gaacaaagca cctggtcgcg catactaggg
       61 gctataaatt tatctttatc agaagccatc acatgaaaga agtcgaaaaa aacgaaatca
      121 aacgtctcag cgatcgtctg gacgccatcc gccaccagca ggccgatctg tcgctggttg
      181 aagccgcaga caaatacgcc gaactggaaa aagagaaagc cacgctggaa gcagaaattg
      241 ctcgcctgcg cgaagttcac agccagaagc tcagcaaaga agcgcagaaa ctgatgaaga
      301 tgccgttcca gcgtgcaatc accaaaaaag agcaggctga tatgggcaag ctgaaaaaaa
      361 gtgttcgcgg actggtggtt gtgcacccaa tgaccgcgct gggccgcgaa atgggcctgc
      421 aagagatgac agggttctca aagactgcgt tttaagttcc tttctcgtcg ctgtaatcac
      481 agcctctcac catttactcg ctcctgctgg cacatgcagg agcctgtttc agagatcgaa
      541 tatttataac gttcttaaca ttatatattc aatctcctct attcttttta gcgcacatgc
      601 caggctaaat tctgaaaatc aggtacaagt taaagaacga tgtatcaata ttaacaatga
      661 attttcgcat atcaatgctt gaaacataat taattattta aatttttcaa tcagcttcgc
      721 aaattccaca ataaaacatc actcaactga a
//
LOCUS       DAPHCV010000395          744 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.401, whole genome shotgun sequence.
ACCESSION   DAPHCV010000395 DAPHCV010000000
VERSION     DAPHCV010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.401"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>744
                     /locus_tag="RH033_005044"
     CDS             <1..>744
                     /locus_tag="RH033_005044"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2994070.1"
                     /translation="SGAAHAWTTGDFNGSFDMNGTITADAYKDKWEWMVGGALSFNNT
                     IKEMTGDSKLLTITQSEPAPILLGRTKEAFAASIVGVGAIPLIAFSDYEGKGVALQSS
                     GDNGKGFFELPMKDDSGNNLGSVKVNVTSAGLFSYSEISTGLVGITSVASGDNTSIYY
                     GGLVSPAIRAGKDAASAVSKFGNYNHTQLLGQLQAVNPNAGNRGQVNKNSAVSQNMVM
                     TTGDVIASSYALGIDQGQTIEATFTNPVV"
BASE COUNT          209 a          134 c          190 g          211 t
ORIGIN      
        1 atcaggtgcg gcgcatgcgt ggaccactgg tgattttaat ggttcatttg atatgaatgg
       61 cacaattact gctgatgcgt ataaagacaa atgggaatgg atggttgggg gcgctctctc
      121 cttcaacaac actatcaagg aaatgacagg tgacagtaag ctgctgacca tcactcagtc
      181 tgaaccagct cctattcttt tagggcgcac aaaagaggcg tttgcagcat cgattgttgg
      241 tgttggtgca attcctttaa ttgcgttcag tgattatgaa gggaaaggag ttgccttaca
      301 gagttctggg gataacggta aggggttctt tgaattgccc atgaaagatg atagtggaaa
      361 taatctcggt agcgtaaaag ttaatgttac ttctgctggc ctgttttcct atagtgaaat
      421 atcaacaggt ttagttggta taacttctgt tgccagtggc gataatacaa gtatttatta
      481 tggtggtctg gtgtcgccag caattagggc gggtaaagac gcagcatcag ctgtgtcgaa
      541 atttggcaac tataatcata cacaattgct gggccagctt caagcagtaa accctaacgc
      601 gggcaataga ggacaagtaa ataaaaatag tgcggtctca caaaatatgg tgatgactac
      661 tggtgatgta attgcatcct cttacgcact tggtattgac cagggacaga ctattgaagc
      721 aacctttact aatcctgtgg ttag
//
LOCUS       DAPHCV010000396          742 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.402, whole genome shotgun sequence.
ACCESSION   DAPHCV010000396 DAPHCV010000000
VERSION     DAPHCV010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.402"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            133..723
                     /locus_tag="RH033_005045"
     CDS             133..723
                     /locus_tag="RH033_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2994071.1"
                     /translation="MSKKLGFALSGIMLAMAAGTAFAANDMDGGQLNISGLVVDNTCE
                     ARVDGGNKDGLILLQTATVAEITDGVLDTTVGAKAKPFSITIDCSKANPAPGTTAKMT
                     FGSVFFSNSKGTLNNDMSINNPSDGVNIALHNIEGSTIKQVQVNNPGDVYSKTLDATS
                     KTATYDFKASYVRADASKAATAGYVKTNSAYTITYQ"
BASE COUNT          216 a          149 c          158 g          219 t
ORIGIN      
        1 tgaatgtaat agttggtaaa caacagacaa acatcattaa cacagtaacg attttgttac
       61 gcagcattgc agcagactct tatcgtcatg cggtgcggtt atctctctat ggaatattat
      121 tcaaggattt ttatgtctaa aaaattaggt tttgcgctgt ctggtattat gttggcaatg
      181 gctgctggta ctgcatttgc tgctaacgat atggacggtg gtcagttaaa tattagcggc
      241 ctggtcgttg ataacacctg tgaagctcgt gttgatggcg gtaacaaaga tggtttgatc
      301 ctgctgcaaa ccgctactgt tgctgaaatc accgatggtg tactggatac caccgtaggt
      361 gctaaagcta agccattcag cattactatt gactgcagca aagctaaccc ggctccgggt
      421 actactgcaa aaatgacttt cggttccgtg ttctttagca atagcaaagg tactctgaat
      481 aacgatatgt ctatcaataa tccatctgat ggtgtgaata ttgccctgca taatattgaa
      541 ggttccacta ttaagcaggt tcaggttaat aatccgggtg atgtttacag caaaactctg
      601 gatgcaacgt ctaaaactgc aacctacgat tttaaagcat cctatgttcg tgctgatgca
      661 agcaaagcag caactgccgg ttatgtaaaa actaactcag catataccat tacttaccag
      721 taaatatcag ccacctaaat tg
//
LOCUS       DAPHCV010000397          732 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.403, whole genome shotgun sequence.
ACCESSION   DAPHCV010000397 DAPHCV010000000
VERSION     DAPHCV010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.403"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..183
                     /locus_tag="RH033_005046"
                     /pseudo
     CDS             1..183
                     /locus_tag="RH033_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            265..531
                     /locus_tag="RH033_005047"
     CDS             265..531
                     /locus_tag="RH033_005047"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2994072.1"
                     /translation="MKPNQLIDRARNKGHQVGQYLDDNSAADFIASVAKKGPGVHDVP
                     LPTNIKSRGYLPDGTEVVPDMARVVVKPDGSVRTSFPFNSSHDN"
     gene            549..>732
                     /locus_tag="RH033_005048"
     CDS             549..>732
                     /locus_tag="RH033_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2994073.1"
                     /translation="MNEIDKICSELGVPVSDKFTQDWAYELPEKYRTKEWLSKYIAAY
                     LNNGYSQKGKNELMTLA"
BASE COUNT          243 a          137 c          162 g          190 t
ORIGIN      
        1 cagaacctgc gttaccaggg acagtacctc gacagggaaa cgggactgca ctacaatctg
       61 cacaggtact atgatccgga tgtcgggcgg ttcatggtaa cggacccgat agggctgaga
      121 ggcgggttaa acttatatca gtatgcacct aatccactgt catggatcga cccacttgga
      181 ttgtgtccta aatctgactt caaatgggga aatccaaaaa gtaagcctac atacggtcac
      241 accttttctg aacacggaca gaaaatgaaa ccaaatcagt taattgatcg tgccagaaat
      301 aaaggtcatc aagttggaca atatctcgat gataattctg ctgctgattt tattgcttct
      361 gttgccaaaa aaggaccagg tgtgcatgat gtgccattgc ctacaaatat aaaaagtcga
      421 ggttatttac ctgatgggac agaggtagtt ccagatatgg caagggttgt tgtaaaaccc
      481 gacggttctg tacgtacatc gttcccattc aattcatcac acgacaacta aaataatgaa
      541 ggatatatat gaatgaaata gataaaatct gtagtgaact tggtgtacca gtgagtgata
      601 agtttacaca agattgggct tatgaactgc ctgaaaaata tcgaactaaa gagtggttaa
      661 gtaaatatat tgctgcatat ttaaataatg gctactcaca aaaagggaag aatgaattaa
      721 tgactttagc cc
//
LOCUS       DAPHCV010000398          729 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.404, whole genome shotgun sequence.
ACCESSION   DAPHCV010000398 DAPHCV010000000
VERSION     DAPHCV010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.404"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>729
                     /locus_tag="RH033_005049"
     CDS             <1..>729
                     /locus_tag="RH033_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV2994074.1"
                     /translation="NGNGIPESITRSITGVDGNAALNISVPYKTSYTQNILSVESSIN
                     IKGGTSNTSIGGAGVYGENFTLNNNGSVWGGDGYNGGIAVSGNKISINNYRNVYGGNG
                     LGGSGSSGGAGLSGDDIIVDNYRSIYGGDDVGGTGGSGVTGSNITVHNSGGILGGNGV
                     NGGDGINGSNLFITNDNMISGGYGIKQGGDAISGNQITLNNNGIVQGGYGPDGGCSVY
                     GEDIHINNHGNLSGLYNSQKDAYN"
BASE COUNT          240 a           89 c          196 g          204 t
ORIGIN      
        1 tgaatggtaa tggtatacct gaaagtataa ccaggagtat tacaggtgtt gatggaaacg
       61 cggcgcttaa tatcagcgtg ccgtataaaa caagttatac tcaaaatata ctatctgttg
      121 aaagcagtat taacatcaaa ggaggaacaa gcaatacatc aatcggtggt gcaggtgtct
      181 acggtgaaaa ctttacgcta aataataatg gtagtgtttg gggaggagat ggatataatg
      241 gtggaattgc tgttagtggc aacaaaatat ctataaacaa ttacagaaat gtatatgggg
      301 gtaatggtct tggtggctca ggaagtagcg gaggtgcagg gttaagcggg gatgatatta
      361 tagttgataa ttacagaagt atatacggag gtgatgatgt aggtgggaca ggtggttccg
      421 gtgtaaccgg tagcaatatt acagtgcata attccggagg aatattgggc ggtaatggcg
      481 taaacggtgg tgatggtatt aatggtagta atcttttcat tactaacgac aacatgatat
      541 ctggaggata tggaataaaa caagggggag atgctatttc tggaaatcaa atcactttga
      601 ataataacgg tattgttcag gggggatatg gccccgacgg tggttgctct gtttatggag
      661 aagatatcca tattaataat catggtaatc tttcaggatt atataatagc caaaaagatg
      721 cttataata
//
LOCUS       DAPHCV010000399          728 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.405, whole genome shotgun sequence.
ACCESSION   DAPHCV010000399 DAPHCV010000000
VERSION     DAPHCV010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.405"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..629)
                     /locus_tag="RH033_005050"
     CDS             complement(<1..629)
                     /locus_tag="RH033_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDV2994075.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCL"
     gene            complement(639..>728)
                     /locus_tag="RH033_005051"
     CDS             complement(639..>728)
                     /locus_tag="RH033_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV2994076.1"
                     /translation="PIRVVCRKWSASVGALWVTITADFEQVVA"
BASE COUNT          151 a          236 c          175 g          166 t
ORIGIN      
        1 ttcaggcatt tgctgcattt atccttcgtg atatcggacg tcggctgatc atattcatcc
       61 gcgacagccg gaccgtgata accgcactca tcaccgcgat aggtccaggt gcaggtatta
      121 gccagcatga tgcgccccgg aaaaacggca ccatccgttt ccgtcggtgt ggacagtaca
      181 aaggaggcac tgaccgcact cagttcgctg cactgctcga tgcgccagcg gctgatcacc
      241 tcctgctccg gatcggcgtc gctgtttccg ttgacgaagt tcaccgcatc cagaaaacgg
      301 gcgtaaacct tacgcctgac caccgttccg ccgaccagac tctgcaggtc ttccgccatc
      361 ccggtgacca tgccgtgcag gttagaaacc gtcagtgtcg gacgggcagc actgcccttg
      421 ccgttcagtt caaatcccgt cccctgaatg gggtatgcct gatactgccg cccctgccag
      481 gtaaccggct cacctttttc gttctgctca ttacagaaaa aataacgttc accaccgacc
      541 tctgtcagat cgatttccca gagcaccacc tgggctgact gagtgaggcg tgtcgtctca
      601 tgatgtgttt cctgtggaat atcctgcatc agagcctcct atgccacgac ctgttcaaaa
      661 tctgccgtta tggttaccca cagcgccccc acgcttgccg accatttacg acaaaccacc
      721 ctgatcgg
//
LOCUS       DAPHCV010000400          725 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.406, whole genome shotgun sequence.
ACCESSION   DAPHCV010000400 DAPHCV010000000
VERSION     DAPHCV010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.406"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            205..663
                     /locus_tag="RH033_005052"
     CDS             205..663
                     /locus_tag="RH033_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2994077.1"
                     /translation="MKIYWTRKSIPELSALPPSLRKKNFTDTYNAASSHIEYWIGAGV
                     SFISMMILFRVYDFLLPAQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILVR
                     GKETETISERLIREADTREYELWRPVRRFFSILFLIVLLVCIHSLITTIK"
BASE COUNT          228 a          129 c          124 g          244 t
ORIGIN      
        1 aagacaccca caaacactct ctttttatta aataaatcac ctgattaaat tataaaagtg
       61 tgatataagt caaaattact gacgaaatca attgataatg attatctcta tcattatcgt
      121 gatattcgag aatgaaaggg gaagtagtca ttataagcct tttataaatc gtcatagtag
      181 aacacttttt atatgagttg ctatatgaaa atatactgga caagaaaaag cattcctgaa
      241 ctttcagcac ttccaccttc attaagaaag aaaaatttta ctgacacata taatgctgca
      301 tcttctcata tcgaatactg gataggtgct ggcgtctcat ttatctcgat gatgatttta
      361 ttcagagtat atgactttct tttacccgcc caagatactt tcccgggtga tatcatcaga
      421 agtttatgtg ttgtgtgtcc atccatatta atctggtttc agttttcggt atatgtcatg
      481 aggaaatatt acaggcatat tctggtgcga ggaaaggaaa ccgaaaccat ttcagaaagg
      541 ttgatacgtg aggctgatac acgagaatat gaactatggc gtccggtccg tcgctttttc
      601 tccattcttt ttctcattgt tctattagtt tgcattcatt cactgataac aactataaaa
      661 taaaagttat agctgttctg ataccggaga agaaacgttc atgctatggt ctcctcttcc
      721 tgtta
//
LOCUS       DAPHCV010000401          725 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.407, whole genome shotgun sequence.
ACCESSION   DAPHCV010000401 DAPHCV010000000
VERSION     DAPHCV010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.407"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>725
                     /locus_tag="RH033_005053"
     CDS             <1..>725
                     /locus_tag="RH033_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001702852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2994078.1"
                     /translation="SREYLNENAYKKRYQSDIHPRKVNVPILQLEVAWINNNCRSSDS
                     STDIIILPRMIRRMVVFNYTKINKQLEKLLGGIARKQTSERNKGGLYTRIKKESDFLS
                     MDRFMVEIENIIRPERFPTSIYPVFSHSFIERELPLLNLARSQYKRAISKKVANSIFY
                     TLQKTNVALWLHLEMFALVAFIKLYTGKGFEFIKNMCAFIDYNNGIIKSTHEELLGEI
                     KHNIQVNLKVNPDNFPELINIYN"
BASE COUNT          278 a          106 c          130 g          211 t
ORIGIN      
        1 cagcagagaa tatcttaatg aaaacgcata caaaaaacga tatcagagtg atattcaccc
       61 cagaaaagtt aatgttccga tactgcaact tgaagttgca tggataaata ataattgccg
      121 ttcaagtgat tcaagcactg atattattat ccttccgagg atgataagaa gaatggttgt
      181 atttaattat acgaagataa ataaacaatt agagaaattg ttagggggga tagcacgaaa
      241 acaaacctca gaaaggaata aaggcggtct ctataccaga ataaaaaaag aaagtgactt
      301 tctttctatg gatagattta tggtggaaat agaaaatatc ataaggcctg aaaggtttcc
      361 gacatcaatt tatccagtct tttcacattc atttattgaa cgagagcttc cgctactgaa
      421 tcttgctcga tcacagtata aaagagcaat aagtaaaaaa gtggcgaatt caatttttta
      481 tactttgcaa aaaactaacg tagcattatg gctacatctt gaaatgtttg ctctggtggc
      541 attcattaaa ctgtatacag gaaaaggttt tgaattcata aaaaacatgt gtgcatttat
      601 cgattataat aatgggataa taaaatccac acatgaagaa ctactgggtg aaattaagca
      661 taacattcag gttaatttga aggtgaaccc tgataatttt cctgagctaa taaatatata
      721 caatt
//
LOCUS       DAPHCV010000402          712 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.408, whole genome shotgun sequence.
ACCESSION   DAPHCV010000402 DAPHCV010000000
VERSION     DAPHCV010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.408"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            142..378
                     /locus_tag="RH033_005054"
     CDS             142..378
                     /locus_tag="RH033_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HDV2994079.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLGRLEQATGEEEQKHYNALIGLLFE"
     gene            421..693
                     /locus_tag="RH033_005055"
     CDS             421..693
                     /locus_tag="RH033_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HDV2994080.1"
                     /translation="MKTSDNESTKYEITGQAVLQILRMKINFSLQTLIKQLLVMKSAE
                     ENAFRRDLIDSIIRDFSNSDTGGPNRRTAIADNKSMFNGKKINRIH"
BASE COUNT          248 a          113 c          142 g          209 t
ORIGIN      
        1 attttttatc ataacatgtt gttttatatg tattagcact aaatgcaaaa aattaattta
       61 tcattttgta cacatatttc gtacaagttt gctattgtta cctcacttaa cattgattaa
      121 catttttaac agaggcgtag catgcatcaa aattcagtga ctttagattc tgcgggagca
      181 atcactcgtt actttgcaaa agctaacttg cctactcagc aggaaactct cggggaaatt
      241 gtgactgaga ttttgaaaga tggacgtaat ctgagtcgaa agtcgctttg tgccaaactt
      301 ctaggccgac tggaacaagc gacaggggaa gaggaacaga aacattataa tgcactaatt
      361 gggttgcttt ttgaataatg caacagcctg attatttaac aggttagcta tgaagtcgtt
      421 atgaagacat ctgataatga aagtacaaaa tatgaaatta ctggccaggc ggtgctccag
      481 atactgcgca tgaagataaa tttttcgttg cagactctta ttaagcaatt actcgtaatg
      541 aaatcggctg aggaaaatgc tttccgacga gatttaattg acagcataat tcgtgatttt
      601 agcaacagtg atacaggagg gccaaacaga agaacagcga tagctgacaa taaaagtatg
      661 ttcaatggta agaaaataaa cagaatacat taaaatttca taagtaagat ga
//
LOCUS       DAPHCV010000403          711 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.409, whole genome shotgun sequence.
ACCESSION   DAPHCV010000403 DAPHCV010000000
VERSION     DAPHCV010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.409"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..602)
                     /locus_tag="RH033_005056"
     CDS             complement(<1..602)
                     /locus_tag="RH033_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDV2994081.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDE"
BASE COUNT          187 a          174 c          163 g          187 t
ORIGIN      
        1 ttttcgtcgc aggcttcaat catgcgtttc gggtccataa acaactgacc ggggcgcata
       61 gggatctcac gcaactccac atcccagtag cgggcgaatt tatgccagca gatttgtacc
      121 ggaccgcaca ccaggtttgg tttatccgtt ggtttgcctg cggcttccat acgcttgcgc
      181 caacgccatt tcatcgccat cccgccgagc atacaggcct cggaagaacc aatggtgttg
      241 gtgccaacgg cctgaccatt tttcggtgca ggcgcatgcc acagatcggc aaccatgttt
      301 acgcaacgca ggtcgattgc agcggattgc ggatattctt ctttgtcgat ccagttttta
      361 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
      421 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctgaaatgca
      481 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      541 tttgcaccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      601 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      661 agcaaggcat tacaggctta atttaaataa caaaatccta agcagaaaaa c
//
LOCUS       DAPHCV010000404          703 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.410, whole genome shotgun sequence.
ACCESSION   DAPHCV010000404 DAPHCV010000000
VERSION     DAPHCV010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.410"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>703
                     /locus_tag="RH033_005057"
     CDS             <1..>703
                     /locus_tag="RH033_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HDV2994082.1"
                     /translation="EDDARPEALRRFELMVEEVARNASAVAQNTAAAKKSASDAGTSA
                     REAATHATDAAGSARAASTSAGQAASSAQSASSSAGTASTKATEAEKSAAAAESSKSA
                     AATSAAAAKTSETNAAASQQSAATSASTATTKASEAATSARDASASKEAAKSSETSAA
                     SSASSAASSATATANSAKAAKTSETNAKSSETAAGQSASAAAGSKTAAASSASAASTS
                     AGQASASATAAGKSAE"
BASE COUNT          176 a          223 c          217 g           87 t
ORIGIN      
        1 gaggatgatg cccgtcctga ggctctgcgc cgttttgaac tgatggtgga agaggtggcg
       61 cgtaacgcgt ccgcggtggc acagaacacg gcagccgcga agaagtcagc cagcgatgcc
      121 ggcacatcag cccgtgaggc ggcaacccat gcgactgatg ctgcaggctc agcacgcgca
      181 gccagcacgt cagccggaca ggccgcgtcg tcggctcagt cagcgtcttc cagcgcagga
      241 acggcatcaa caaaggccac tgaagcggaa aaaagtgctg ccgctgcaga gtcctcaaaa
      301 agcgcggcgg ccaccagtgc cgctgcggcg aaaacgtcag aaacgaatgc ggcagcgtca
      361 caacaatcag cagccacttc tgcatccacc gcgaccacga aagcgtcaga agctgccacc
      421 tcagcccggg atgcgtcagc ttcaaaagag gcggcaaaat catcagaaac gagcgcagcc
      481 tcgagcgcca gcagcgcagc ttcctcggca acggcgacag caaattctgc gaaggcggcc
      541 aaaacgtccg agacgaacgc taagtcttct gaaacggcag cgggacagag tgcctcagct
      601 gcggcaggct caaaaacagc ggctgcgtcg tctgccagcg ccgcgtcaac aagtgccggg
      661 caggcctcag ccagtgccac cgctgccgga aagtcggcag aaa
//
LOCUS       DAPHCV010000405          702 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.411, whole genome shotgun sequence.
ACCESSION   DAPHCV010000405 DAPHCV010000000
VERSION     DAPHCV010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.411"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(94..594)
                     /locus_tag="RH033_005058"
     CDS             complement(94..594)
                     /locus_tag="RH033_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDV2994083.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDETDPELMFQDYEGFPGNMASECHINWAWVEGFRQARDEGCEEAYRLWVDDTGETD
                     FDSFRDAWWGEADSEEAFAVEFVSDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
BASE COUNT          165 a          227 c          185 g          125 t
ORIGIN      
        1 ggcgcggccc tcctgcattt caggcaggcg taagcctgcc agggttttcc ggcggcctgg
       61 caggccatac ccccgccgga gcgggggagt aaatcaccga cggaacacat gaccgtcaat
      121 aaaggtgaag gagtccagga ataaatcccg cgcatacgcc tcatagtcaa aatagagcgc
      181 caccgtctcc ggcacgtcag ccagcaggcc ggtatcactg acgaactcaa ccgcaaaagc
      241 ctcctcactg tcagcctcgc cccaccaggc atcgcggaag ctgtcaaaat ccgtctcacc
      301 ggtatcatcc acccagagac gataagcctc ttcgcagcct tcatcccgcg cctggcggaa
      361 gccttcaacc caggcccagt tgatatggca ttcagaggcc atattccccg ggaatccctc
      421 ataatcctga aacatcagtt ccgggtcggt ttcatcctgg tgaagggcac ggcaggcggc
      481 gaaaaagtcg cgttcatcat caaacgttgt caggtcaaac cagcggccag cgatgcttcc
      541 acagttgtat ttgtgccagg ttccaacgta tacagcaggt gcaacaacag acattcccga
      601 atcctccata gtcacatgtt ctgcgacacc gggcggtgcc gtgcctccat ctttgccggg
      661 cctgcaggga tgcaagggcg caagggaagc cgcagcccga cg
//
LOCUS       DAPHCV010000406          702 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.412, whole genome shotgun sequence.
ACCESSION   DAPHCV010000406 DAPHCV010000000
VERSION     DAPHCV010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.412"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..602)
                     /locus_tag="RH033_005059"
     CDS             complement(<1..602)
                     /locus_tag="RH033_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDV2994084.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDE"
BASE COUNT          182 a          170 c          159 g          191 t
ORIGIN      
        1 ttttcgtcgc aggcttcaat catgcgtttc gggtccataa acaactgacc ggggcgcata
       61 gggatctcac gcagctccac atcccagtag cgggcgaatt tatgccagca gatttgcacc
      121 ggaccgcaca ccaggtttgg tttatccgtt ggtttgcctg cagcttccat acgcttgcgc
      181 caacgccatt tcatcgccat cccgccgagc atacaggcct cggaagaacc aatggtgttg
      241 gtgccaacgg cctgaccatt tttcggcgca ggcgcatgcc acagatctgc aaccatgttt
      301 acgcaacgca ggtcgatggc tgcagattgc ggatattctt ctttgtcgat ccagtttttg
      361 ttaatggata aatccatcaa cttatggaca ttttcgtcgt cccaggtctg gcagaaagtg
      421 gccaggttct gacgagcgtt gccatcaaga tataattcgt cattgataat ctggaatgcg
      481 acatcatcgc gcatttcgtg cagcggaaaa cgttttgatt ctgcgatagt ggaaatagac
      541 ttagcaccaa aacgtgaatc gagtagttcc gaccttaaat ccgttacttg cttcttatcc
      601 attttaaact ccttaaaatg attggatcgc attaaaaaag taggatttat cgataaagta
      661 agcaagtgca aaggactcgt gtttaaataa caaaatccta at
//
LOCUS       DAPHCV010000407          699 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.413, whole genome shotgun sequence.
ACCESSION   DAPHCV010000407 DAPHCV010000000
VERSION     DAPHCV010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.413"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>699
                     /locus_tag="RH033_005060"
     rRNA            <1..>699
                     /locus_tag="RH033_005060"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          158 a          153 c          232 g          156 t
ORIGIN      
        1 tgtttgatgt tctaacgttg gcccctgatc ggggttgcgg acagtgtctg gtgggtagtt
       61 tgactggggc ggtctcctcc taaagagtaa cggaggagca cgaaggttgg ctaatcctgg
      121 tcggacatca ggaggttagt gcaatggcat aagccagctt gactgcgagc gtgacggcgc
      181 gagcaggtgc gaaagcaggt catagtgatc cggtggttct gaatggaagg gccatcgctc
      241 aacggataaa aggtactccg gggataacag gctgataccg cccaagagtt catatcgacg
      301 gcggtgtttg gcacctcgat gtcggctcat cacatcctgg ggctgaagta ggtcccaagg
      361 gtatggctgt tcgccattta aagtggtacg cgagctgggt ttagaacgtc gtgagacagt
      421 tcggtcccta tctgccgtgg gcgctggaga actgaggggg gctgctccta gtacgagagg
      481 accggagtgg acgcatcact ggtgttcggg ttgtcatgcc aatggcactg cccggtagct
      541 aaatgcggaa gagataagtg ctgaaagcat ctaagcacga aacttgcccc gagatgagtt
      601 ctccctgaga ctttaagtct cctgaaggaa cgttgaagac gacgacgttg ataggccggg
      661 tgtgtaagcg cagcgatgcg ttgagctaac cggtactaa
//
LOCUS       DAPHCV010000408          696 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.414, whole genome shotgun sequence.
ACCESSION   DAPHCV010000408 DAPHCV010000000
VERSION     DAPHCV010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.414"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(1..479)
                     /locus_tag="RH033_005061"
                     /pseudo
     CDS             complement(1..479)
                     /locus_tag="RH033_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519082.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rhs family protein"
BASE COUNT          176 a          169 c          132 g          219 t
ORIGIN      
        1 gggtcattga tcttctgata tctatcactt ttgaaatcgt atgttctagg atcagcaccc
       61 ggtttaggtt gtcctgcatg tgcgtgaggg ccaggtgccc taggatctgc cgtgtgctcg
      121 actacaactt tcttacgtcc gtaggcatct gtatattcat aatatcgccc atgagaccct
      181 aaatcttccg aatatttata atttgtttgt ccccttcttc taggatcatt tccaacggtc
      241 cattgcctat taggttgctg tgaacgaggt atgcccgcaa gatcttttgc tttatttagt
      301 gcctgacgac ggtttcttaa ttcaccacat ttaagaccaa gcggatctat ccagcttact
      361 ggattaggcg cgtaagcata taagtttagc ccccccgcca gccctatcgg gtcgggttac
      421 gtaaatgttc cacagtccga gtcgtagtac ctcagaaggt tgcagtgcaa tcccgccttg
      481 cgacatcaag gcatagtttc agaaaaagta aggagcaatc accatacccc aattcactgg
      541 gtgctaaata atatctggta acatccctta cttcaattct atttcattac aacttcttta
      601 ctttccatat tctttgcgca gctaaaaaag atactccttt gaaccaagac gcacattcaa
      661 taacttgttc ccatgaatat attttatgtg agctaa
//
LOCUS       DAPHCV010000409          688 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.415, whole genome shotgun sequence.
ACCESSION   DAPHCV010000409 DAPHCV010000000
VERSION     DAPHCV010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.415"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..492
                     /locus_tag="RH033_005062"
     CDS             <1..492
                     /locus_tag="RH033_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HDV2994085.1"
                     /translation="AICLMFINEICHATDCFDLAGRDYKIDPDLLRAISWKESRYRVN
                     AIGINPVTGYGSGLMQVDSQHFNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWC
                     VSWEAVGAYNAGFRKTERQNQRRLAYASDVYRIYTGIKNSKGIRIPATKKSLPEINSV
                     QNN"
BASE COUNT          207 a          163 c          158 g          160 t
ORIGIN      
        1 gccatctgcc tgatgtttat aaatgagatc tgccatgcca ctgattgctt tgatcttgca
       61 ggccgggatt acaaaataga tcctgattta ctgagagcaa tatcatggaa agaatcccgt
      121 taccgggtta atgccatcgg tattaatccg gtaacgggat atggcagcgg actgatgcag
      181 gtagattccc agcattttaa cgaactggcc cgctatggaa ttaagccgga acatctgaca
      241 acagatccct gcatgaacat ttataccggt gcttattatc tggcaatagc ctttaaaaaa
      301 tggtgcgtct cctgggaggc cgttggtgca tacaatgctg gattcaggaa gaccgaacgc
      361 cagaaccaga gacgtcttgc ctacgcatca gatgtttacc ggatttatac cgggataaaa
      421 aacagtaaag gtatccggat tccggccacg aagaaatcac ttccggaaat taacagcgtg
      481 cagaacaatt aatcttacag acattgtcag ataccggtta tgccgcaaaa gcggcatgga
      541 aggccacaca caaaaatatc atatgagtga caatactgac tggccgaagc cccgcgaagc
      601 ggtaacccat cttatatata cccggtaact gcgcagcctc ctgccagacc atttttcgcg
      661 ggcacaatat ttacagacaa ccggaaag
//
LOCUS       DAPHCV010000410          687 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.416, whole genome shotgun sequence.
ACCESSION   DAPHCV010000410 DAPHCV010000000
VERSION     DAPHCV010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.416"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..556)
                     /locus_tag="RH033_005063"
     CDS             complement(<1..556)
                     /locus_tag="RH033_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HDV2994086.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSVNSVAEVLDSCQGYDIKASCQ
                     ASRQSLSGITQDWSIADGQWVIFSGMANNASGGAVFLQQSAEFTISPQNETGMTLFAN
                     NSISGEYNNGGAIFAKENSTINLANVIFDSNVAGGYGGAIYSAGTNDAGDMDLNITNA
                     IFENNIANDGKGGAIYSINNDIYLS"
BASE COUNT          204 a          152 c          116 g          215 t
ORIGIN      
        1 cacttaaata aatatcatta ttgatagaat aaatagcgcc acctttacca tcattggcga
       61 tattattctc aaatattgca ttagtgatat ttaaatccat atcaccggca tcgtttgttc
      121 ccgcagaata gattgctccg ccatagcctc ctgcaacgtt actgtcgaaa ataacattcg
      181 caagatttat cgttgagttt tctttagcaa agatagctcc gccattatta tattcgccac
      241 taatcgagtt attagcaaac agggtcatcc ctgtttcatt ttgtggtgat atcgtaaatt
      301 cagcactttg ctgcaaaaat acggcgccac cgctggcatt attggccata cccgaaaaaa
      361 tcacccattg cccatcggcg atgctccagt cctgcgtaat gcctgaaagg ctttgcctgc
      421 tggcctgaca actcgctttg atatcatatc cctggcatga atccaaaacc tccgcgacac
      481 tgttaacaga gaaaagagat gcaatcattg acgggagtaa agataaatac tccttgcgta
      541 gaaaaataat ccgcatatta atctgaccat cattttctat aagacggcat atttaatcgc
      601 attatacgat atgggttatt tttcttttgt aaattgttca acccagtgca atggctattt
      661 tgttattatt tcatgaataa tatcagt
//
LOCUS       DAPHCV010000411          687 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.417, whole genome shotgun sequence.
ACCESSION   DAPHCV010000411 DAPHCV010000000
VERSION     DAPHCV010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.417"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>687
                     /locus_tag="RH033_005064"
     CDS             <1..>687
                     /locus_tag="RH033_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HDV2994087.1"
                     /translation="ADLPTSQFWSYATYFRVLSFEYLSESISTLLYLDADVVCKGSLK
                     PLTEIIFKDEFAAVIPDNDSTQAACAKRLNIPEMNGRYFNAGVIYVNLKKWHEANLTP
                     YLLTLLRGETKYGSLKYLDQDALNIAFNMNNIYLAKDFDTIYTLKNELHDRSHRKYQQ
                     TITDKTVLIHYTGITKPWHSWAGYPSASYFNIAREQSPWKKYPLKEARTVAEMQKQYK
                     HLFAHGEYIKG"
BASE COUNT          216 a          132 c          137 g          202 t
ORIGIN      
        1 tgctgatctg ccgacgtcac agttttggtc gtacgcaaca tacttcaggg tattgtcttt
       61 tgaatatctg agtgaaagta tttctacact gctgtatctg gatgccgatg ttgtttgtaa
      121 aggaagcctg aaacctctca cagaaattat atttaaagat gagtttgctg cggtcattcc
      181 tgacaatgat agtactcagg cggcatgtgc aaaacgcctc aacattcccg aaatgaatgg
      241 acgttatttc aatgcaggcg ttatctatgt caatcttaaa aaatggcatg aagcaaattt
      301 gacaccgtat ttactcacgc ttttacgagg ggaaactaaa tatggctctc ttaaatattt
      361 agatcaggat gcgttgaata tcgcatttaa tatgaataat atctacctcg cgaaggattt
      421 tgatactatt tataccctga aaaacgaact tcatgatcgt agtcatcgaa agtatcagca
      481 aaccattacc gataaaacag tattgattca ctatacaggg ataactaaac catggcatag
      541 ctgggctgga tatccatctg catcatactt taatatcgcg cgtgaacaat ctccctggaa
      601 gaaatatcct cttaaagagg cgcggactgt tgcagaaatg cagaaacaat ataagcatct
      661 gtttgcccat ggtgagtata ttaaagg
//
LOCUS       DAPHCV010000412          686 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.418, whole genome shotgun sequence.
ACCESSION   DAPHCV010000412 DAPHCV010000000
VERSION     DAPHCV010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.418"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>686
                     /locus_tag="RH033_005065"
     CDS             <1..>686
                     /locus_tag="RH033_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2994088.1"
                     /translation="LNNSQDLNDSQELNNSQDLNNSQVSCKGSVDSTITDLLEKALNK
                     SLLAIRNEHLLLMPHVCDESISYLLGENGILEEIDKLYELNDHGIDNDKEGNNEINDI
                     MINLSHILIDSLDDAKVNLTPVIHSMLVTFLELPYNNDVKILEWCFNKSMQYFDDSAK
                     IEHACFVINHINFLCDQSKVAETLFFNLDKEPYKNSPELQELIWKKLVVYVNDFNLSN
                     LEKTYLIQRI"
BASE COUNT          249 a           94 c          122 g          221 t
ORIGIN      
        1 gttaaataat tcgcaggatt taaatgattc tcaggagtta aataactcgc aggacttaaa
       61 taactcgcag gtgagttgta aaggttcagt tgattctacg attacggatt tattagaaaa
      121 agcattgaat aaatccttat tagcaataag gaacgaacat ctgctattaa tgcctcatgt
      181 atgtgatgaa tcgatttcat acttactggg cgaaaatggt atacttgaag aaatagataa
      241 gctctacgaa ttaaatgatc acggaattga taatgacaaa gaaggtaaca atgaaattaa
      301 tgacatcatg attaacctgt ctcatattct tattgattcc ttagatgatg caaaggttaa
      361 ccttacaccg gtcatccatt cgatgttggt gactttttta gaattgccat ataataatga
      421 tgtaaaaata ctggagtggt gttttaataa aagcatgcaa tattttgatg attctgcaaa
      481 gatagagcat gcatgcttcg taataaatca tattaatttt ctctgcgatc agtctaaagt
      541 agctgagaca ttatttttca atctcgataa agaaccctat aaaaatagcc ctgaattaca
      601 ggagttgatt tggaaaaagt tggttgtata tgtcaatgat tttaacttaa gcaatctaga
      661 aaaaacatat ttaatacaaa ggatat
//
LOCUS       DAPHCV010000413          682 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.419, whole genome shotgun sequence.
ACCESSION   DAPHCV010000413 DAPHCV010000000
VERSION     DAPHCV010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.419"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..605)
                     /locus_tag="RH033_005066"
     CDS             complement(<1..605)
                     /locus_tag="RH033_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDV2994089.1"
                     /translation="MCALDRRERPLNSQSVNKYILNVQNIYRNSPVPVCVRNKNRKIL
                     YANGAFIELFSREDKPLSGESYIRLQVEIFLSSLELECQALGHGSAFCRRFNFHGEIY
                     QIRMENVSFYNDESVVLWQINPFPDYPFFALNQSVSNTNTSGQLTIWNDLSPGTLVVF
                     SFYMLGVGHATIARELGITDRASEDRIKPVKRKIKEFFEHF"
BASE COUNT          224 a          139 c          122 g          197 t
ORIGIN      
        1 tcaaagtgtt caaaaaactc ttttattttc cgtttaactg gtttaattcg atcctcagat
       61 gctctgtctg taatacccaa ttctctggca attgttgcgt gaccaacacc cagcatataa
      121 aaagagaaaa caaccaatgt tcctggagaa agatcattcc atatcgttaa ttgaccagaa
      181 gtatttgtat tacttacact ctgatttaac gcaaaaaatg gataatcagg aaacggatta
      241 atttgccata aaacaacaga ttcgtcatta taaaaagaaa cattctccat ccttatctga
      301 tagatttcgc catgaaaatt aaaacgacga caaaatgcag agccatgtcc aagagcctgg
      361 cattccagtt caagtgatga aagaaaaatt tcaacctgca gacgtatata actctctccg
      421 gataaaggtt tatcttctct ggaaaagagt tcaataaaag ccccattggc ataaaggatt
      481 ttccggtttt tgttacggac acaaaccgga acgggagaat ttctgtagat attctgaacg
      541 ttaaggatgt atttatttac agattgactg ttaagtggcc tttctctacg atccagcgca
      601 cacataggaa cctccactaa ggattctatg gacagtcgat gcagggagtt acagctccct
      661 gcatcggcga ttttcagttt ca
//
LOCUS       DAPHCV010000414          677 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.420, whole genome shotgun sequence.
ACCESSION   DAPHCV010000414 DAPHCV010000000
VERSION     DAPHCV010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.420"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>677)
                     /locus_tag="RH033_005067"
     CDS             complement(<1..>677)
                     /locus_tag="RH033_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HDV2994090.1"
                     /translation="EAVGRASDDAEGYLDFFKGKITESHLGKELLEKVELTEDNASKL
                     EEFSKEWQDANDKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIA
                     FIDPANGNETPMFVAQGNQIFMNDVFLKRLTAPTITSGGNPPAFSLTSDGKLTAKNAD
                     ISGSVNANAGTLNNVTVNENCTIKGMLEATQVRGDFVKAVSKSFPKQAGTWGNTETPN
                     GTVTVTI"
BASE COUNT          130 a          203 c          152 g          192 t
ORIGIN      
        1 ctgatggtga ctgtaaccgt cccgtttggt gtttccgtgt taccccacgt accagcctgt
       61 ttcgggaatg atttggatac agctttaacg aagtcacctc tgacctgagt cgcctccagc
      121 atgcccttaa tcgtacagtt ttcatttacc gtgacattgt tgagcgtccc ggcgttcgca
      181 ttcacactgc cactgatatc cgcattttta gcggtcagct ttccgtctga cgtcagggaa
      241 aatgccggtg gatttccacc actggtaatg gtcggggccg tcaggcgctt caggaacacg
      301 tcgttcataa atatctgatt gccctgcgcc acaaacatcg gcgtttcatt cccgtttgcc
      361 gggtcaataa acgcgatacg gttagcggca accagaaact ggctcagctt gccttcttcc
      421 gtgtcctcca tgctgaggcc aatacccgcg acataatgtt tgccgtcttt ggtctgctca
      481 attttgacgc cccacatggc attccactta tcgttagcgt cctgccactc tttcgaaaac
      541 tcctccagtt tgctggcgtt atcctccgtc agctcgactt tttccagcag ctccttgccg
      601 agatgggatt cggttatctt gcctttgaaa aaatccaggt aaccttccgc atcatcgctc
      661 gcccgaccga cggcctc
//
LOCUS       DAPHCV010000415          676 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.421, whole genome shotgun sequence.
ACCESSION   DAPHCV010000415 DAPHCV010000000
VERSION     DAPHCV010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.421"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            102..>676
                     /locus_tag="RH033_005068"
     CDS             102..>676
                     /locus_tag="RH033_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HDV2994091.1"
                     /translation="MKIALMNEFSQASKNAIIFNELVAASAHQGHTIFNVGMCDDNDH
                     RLTYVHLGIISAILLNSRSVDFVITGCGTGQGALMALNTFPGVTCGYCIEPTDAYLFA
                     QVNNGNALSLPFAKGFGWGAELNMRYIFEKAFDGEKGLGYPAERRESQNANAIILSNM
                     KEAVSKPLMDALQAIDPELLKQALGGEKFQK"
BASE COUNT          204 a          115 c          154 g          203 t
ORIGIN      
        1 ttcaaataca atgttgcaag taatgtttgt aatgtattat catatcgttc atgttgttat
       61 gctatgaagt ttaacagtat gattttaaat gaggaattcg tatgaaaatc gcattgatga
      121 atgaatttag ccaggcatct aaaaacgcaa taatttttaa tgagttagtt gcagcaagcg
      181 cacatcaagg ccatactatt ttcaatgtag gaatgtgtga tgacaatgat catcgtctga
      241 cttacgttca tctcggcatt atttcggcta tcttgttaaa cagcagatct gttgatttcg
      301 ttattactgg ctgtggtaca ggacaggggg cattgatggc actgaacaca ttcccgggag
      361 ttacttgtgg ttattgcatc gaaccaacag atgcctacct gtttgcacag gtaaataatg
      421 ggaatgcact gtccttgcca tttgcaaagg gttttggctg gggggcagaa ttaaacatgc
      481 gctatatttt tgaaaaagca tttgatggcg aaaagggatt aggctatccg gcagaacgtc
      541 gtgaatcaca aaatgcgaat gccattatac tgagtaatat gaaagaagca gttagtaagc
      601 cactcatgga tgctctccag gccatcgatc cggagttact taaacaagct cttggtggcg
      661 aaaaattcca gaaatg
//
LOCUS       DAPHCV010000416          674 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.422, whole genome shotgun sequence.
ACCESSION   DAPHCV010000416 DAPHCV010000000
VERSION     DAPHCV010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.422"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..139
                     /locus_tag="RH033_005069"
     CDS             <1..139
                     /locus_tag="RH033_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2994092.1"
                     /translation="VLPGERVMMAFTYARTTPLTPADMTRWAEIKKNLRFRMRQEAGT
                     N"
     gene            158..>674
                     /locus_tag="RH033_005070"
     CDS             158..>674
                     /locus_tag="RH033_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2994093.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLAASVGITMGCTAVVLTGFVAGVAMVYTGVTDTVSEVCSDLANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFLDVAGEYLEM
                     AGAIALAVNPVT"
BASE COUNT          142 a          171 c          236 g          125 t
ORIGIN      
        1 tgtattgccg ggtgagcggg tcatgatggc cttcacctac gcccgaacca caccgctgac
       61 cccggctgat atgacgcgct gggctgaaat taaaaagaat ctgcgcttca ggatgcggca
      121 ggaagccggg acgaattaat cacacaggga acgggacatg gaagaggcat tctgggcggc
      181 aaggcagggt gatgccctgc tgcatacgtc gtttatggca gatgtgctgg gggcggtgct
      241 ggaggtggcc gcaaatgtcg ttattgatgc cctcattgtc ggcacaacct cgttactggc
      301 ggcgtcagtg gggatcacga tggggtgcac ggccgtcgtg ctgaccggtt ttgtggcggg
      361 tgtggcgatg gtgtacaccg gcgtgacaga cacggtcagc gaagtctgtt cagacctcgc
      421 aaatatgctt ttcccgcctc agattgaagg ttacatcctc accggctctg gcgacacatg
      481 gatcaacagc aaacctgccg cgcgggccgc ggcgactgcg gcatcgcggc aggacattga
      541 ggcgcaggag gcgcaggcga aggcggaaca ggaagaagcg cagcgtcagg aggatgccag
      601 aacgttcctg gatgtggccg gggaatatct ggaaatggcg ggggccatag ccctggcggt
      661 taacccggtg acag
//
LOCUS       DAPHCV010000417          673 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.423, whole genome shotgun sequence.
ACCESSION   DAPHCV010000417 DAPHCV010000000
VERSION     DAPHCV010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.423"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..242
                     /locus_tag="RH033_005071"
     CDS             <1..242
                     /locus_tag="RH033_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2994094.1"
                     /translation="SCSDRTLNDYAVIFIRNALNASEHIIIFNCTGWSDLNNESAVMQ
                     FARVARKSGKQLIVAVREQDMKKMEAEFGRIIKIH"
     gene            465..671
                     /locus_tag="RH033_005072"
     CDS             465..671
                     /locus_tag="RH033_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HDV2994095.1"
                     /translation="MKYLNTTDCSLFLAERSKFMTKYALIGGLAVCATVLCFLLIFRE
                     RLCELNIHRGNTVVQVTLAYEARK"
BASE COUNT          190 a          129 c          180 g          174 t
ORIGIN      
        1 acagttgttc tgacagaaca ttaaatgatt atgctgttat ttttatcagg aatgcgctga
       61 acgccagtga gcatattatt atttttaatt gtacaggatg gtctgatttg aataatgaat
      121 ctgccgtcat gcagtttgcc agagtggcac gaaaatcagg aaaacaactg atagtcgcag
      181 taagagaaca ggatatgaaa aaaatggaag cagagtttgg aaggatcata aaaatccact
      241 gacgccttcg cgttgctcag ttgtccaacc ccggggacgg gaaaaagcaa gttttccccg
      301 cccctggcgt ttctgtagtg aaaagcggga aatggaggga atggtcagta acagggaatg
      361 tggtattatc gcggcgggtg tctgagcctt tctggttcag gcaagacgca ggtaccagaa
      421 atgcgaagac cccacttgtt aatccattaa ctcgtgaggc ctgcatgaag taccttaaca
      481 ctactgattg tagcctcttc cttgcagaga ggtcaaagtt tatgacgaaa tatgccctta
      541 tcggggggct cgccgtgtgc gccacggtgt tgtgtttttt actgatattc agggaacggt
      601 tatgtgagct gaatattcac aggggaaata cagtggtgca ggtaactctg gcctacgaag
      661 cacggaagta agc
//
LOCUS       DAPHCV010000418          673 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.424, whole genome shotgun sequence.
ACCESSION   DAPHCV010000418 DAPHCV010000000
VERSION     DAPHCV010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.424"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..553
                     /locus_tag="RH033_005073"
     CDS             <1..553
                     /locus_tag="RH033_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HDV2994096.1"
                     /translation="SAALVISLTGNIIQYWHNSNVTREYFATDNGRLVRLAPTSQPAW
                     SQSDVMTFGSMTLSEAFNLDFVHYRKQITTLAPRFSEAGFAGYVNALQASNILDTIKK
                     EKFNLTATIGAGTLVNQGQMENGIWFWKFQYPVRMRLVGQTSTRPEQAFTFEITVRRV
                     DPRIKPGGMEISQMVSRNAASGT"
BASE COUNT          164 a          203 c          171 g          135 t
ORIGIN      
        1 cagcgcagct ctcgtcatca gtctgaccgg caatatcata cagtactggc ataacagcaa
       61 cgtaacgcgt gaatactttg ccaccgacaa tggccggctg gtccgcctgg cccccaccag
      121 tcagccagcc tggagtcaga gcgacgtcat gacgtttggc agcatgacgc tgagcgaagc
      181 ttttaatctg gattttgtcc actacaggaa acaaatcacg acactggccc cccgcttttc
      241 cgaagccggt tttgccggct atgtgaatgc tcttcaggcc tccaacattc tggacaccat
      301 taaaaaggag aaatttaacc tcacggccac catcggggcc ggcacgctgg tgaatcaggg
      361 ccagatggaa aacggcatct ggttctggaa attccagtat ccggtcagga tgcgtctggt
      421 cggtcagacc agcacccgcc ccgagcaggc cttcaccttt gaaatcactg tccggcgcgt
      481 tgacccacga atcaaacccg gcggcatgga aatcagccag atggtttccc gtaacgccgc
      541 atcaggaacc tgagactcat atcatgcaaa tacatcatct tcttggcgca ctgcttattg
      601 tgtgcggcac tgtcacggct gcagataacc cgggctggca gaacgcacga accccacagg
      661 ctggcggaaa gac
//
LOCUS       DAPHCV010000419          656 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.425, whole genome shotgun sequence.
ACCESSION   DAPHCV010000419 DAPHCV010000000
VERSION     DAPHCV010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.425"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>656
                     /locus_tag="RH033_005074"
     CDS             <1..>656
                     /locus_tag="RH033_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004597459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDV2994097.1"
                     /translation="GVDYVFHAAALKQVPSCEFYPMEALKTNVIGTENVLNAAINNEV
                     KKVVLLSTDKAVYPINAMGISKAMAEKILVAKSRIVGSSGPIMCATRYGNVMASRGSV
                     IPLFVEKIKQGKPLTITDPNMTRFLMSLEDSVDLVLHAFAYGQQGDIFVQKAPASTVN
                     ELSVALCDIFNSQLNYKIIGTRHGEKLYETLVSREEMAKAQDMGRYFRIPADNRDLNY
                     "
BASE COUNT          216 a          103 c          143 g          194 t
ORIGIN      
        1 ggcgtagatt atgtttttca tgctgcagcc ctgaaacaag tgccatcgtg cgaattctat
       61 ccaatggagg ccttaaagac aaatgtcatt gggacagaaa acgtattgaa tgctgcaatt
      121 aataatgaag taaaaaaagt tgttttgtta agtacagaca aggctgtata tcctattaat
      181 gcaatgggaa tatcgaaagc aatggcggaa aagattttag ttgctaaatc acggatcgtt
      241 ggaagtagtg gaccaattat gtgtgctacc agatatggta atgtaatggc gtcaagaggc
      301 tccgttattc ctctttttgt cgaaaaaatt aagcaaggca aaccattaac cattacagat
      361 cctaatatga ctcggttttt aatgtcttta gaggattctg ttgacttggt tctccatgct
      421 tttgcctatg gacagcaagg tgacattttt gttcaaaaag ctcctgcaag taccgttaat
      481 gaattatctg ttgcgctatg tgatattttt aattcgcagt tgaattataa aataatcggg
      541 accagacacg gagaaaaact ttatgaaaca ctagtatcaa gagaggaaat ggcaaaagca
      601 caagatatgg ggcgctactt tagaataccg gcagataata gggatttaaa ttatga
//
LOCUS       DAPHCV010000420          644 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.426, whole genome shotgun sequence.
ACCESSION   DAPHCV010000420 DAPHCV010000000
VERSION     DAPHCV010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.426"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(4..429)
                     /locus_tag="RH033_005075"
     CDS             complement(4..429)
                     /locus_tag="RH033_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2994098.1"
                     /translation="MIRIIVFLLFFLSGCTSQKEYSNIVRIENRCSSDIDVFIQGYVS
                     GSTLIYQHNIHLPVGDSDVLVYFPTADIDDGFKYEGAFMIYKDSRGELDYPFTISLSG
                     REKKFGKNDLIRLSRDISTKDDRKYGVIRYIIDSQEICP"
BASE COUNT          200 a          145 c           92 g          207 t
ORIGIN      
        1 acatcacggg caaatttcct gactgtcgat tatatatctt ataactccgt attttctgtc
       61 gtcttttgtt gaaatatctc gtgataatct aattaagtca ttcttcccaa attttttttc
      121 gcgccctgac aatgaaatgg tgaatggata atccaattca ccgcggctat ctttatatat
      181 catgaatgcc ccctcatatt taaaaccatc atcaatatct gccgtgggaa aatatactaa
      241 tacatcacta tcaccaactg gcaagtgtat attatgctga taaatcagcg tagaaccgga
      301 aacatatccc tgaataaaga catcgatatc tgaggagcat ctattctcaa tcctgactat
      361 attggaatac tctttttgtg aagtacaccc actcaaaaaa aacaataaaa aaacaattat
      421 tctaatcatt aaaactcacc ctgctcaacc agatcaatgc ctgaacatct tcatctgccg
      481 cgaccaatct cttatatcta tcaggtgcat agctatctgc atatttcatt atatcaatct
      541 ctcctgaaga tggcattgga gtattaggga tggtactttg accccgcgtg gatgtcccct
      601 tatgtgtcca tgattcagaa ccatatttat ccataacttt atgt
//
LOCUS       DAPHCV010000421          635 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.427, whole genome shotgun sequence.
ACCESSION   DAPHCV010000421 DAPHCV010000000
VERSION     DAPHCV010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.427"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(1..387)
                     /locus_tag="RH033_005076"
                     /pseudo
     CDS             complement(1..387)
                     /locus_tag="RH033_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          215 a          115 c          123 g          182 t
ORIGIN      
        1 atgacatgaa tcgctcccct gcggcgactc ttgtcataag aatgccggag cgtttttcca
       61 tcaattgcaa taacgtcttt atcatctgaa gaatggcagt cacgcatcca gttaataaag
      121 cactcgtgaa attttgaagg actgatacag gatacaactc tggcgatggt atcgtgaaca
      181 ggaataccat tttaaaaatc accatattgt tttaaaaaat cgagatgtgt ttccccaaaa
      241 tcctctatat cttcccaacc atctgcacca gaaataacgg cacaaatagt caacagtaga
      301 atatccgaca atttatgttc cacttttcca ggattgtctg taatcgggaa taacagaaat
      361 atgttccatc aattttttaa gtaccatttt gttctcctta attatgtaag gagtatttga
      421 tcatgtataa gcaataaaaa acagctttag gtaatgagga atatctcaat ttttaaacat
      481 aaaatgcgaa ttgtctagta caaaaaagca gcgttacgtg atctttccct gagaggtaca
      541 ggaactgcgg gaggaacttt atcaggttaa acaggggtta aggggatgcg gaacgggatg
      601 tttagtgtgc tgaacgaaag ctaaataata aaaaa
//
LOCUS       DAPHCV010000422          627 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.428, whole genome shotgun sequence.
ACCESSION   DAPHCV010000422 DAPHCV010000000
VERSION     DAPHCV010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.428"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>627)
                     /locus_tag="RH033_005077"
     CDS             complement(<1..>627)
                     /locus_tag="RH033_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine methyltransferase"
                     /protein_id="HDV2994099.1"
                     /translation="SDIDYYRSARYNETQLRTDFLDQLFLILGWDITNAAGKPTNERE
                     VLVEEGLKARAGENTKKPDYTFRLFSERKFFLEAKKPSVDISTTIEPALQVRRYGFTA
                     KLKISVLSNFEYTAIYDCSNQVKETDSVANSRIKLYHFTELVDKFDEINNLIGRESVY
                     TGHFDNEWSEIENKILRFSVDDLFLKQINDWRLLLANEFLQIKNELPE"
BASE COUNT          179 a          117 c          111 g          220 t
ORIGIN      
        1 cttctggaag ttcgtttttt atctgcaaaa attcattagc aagaagtaag cgccaatcat
       61 taatttgctt gaggaataga tcatcaacac tgaatctcag tatcttattt tcaatttctg
      121 accattcatt gtcaaaatga ccagtgtaaa cactttctcg accgataagg ttattaattt
      181 catcaaactt atctacgagt tcagtgaagt gatacaactt gattctagag tttgctactg
      241 aatcagtttc tttaacctga ttagagcaat cataaattgc cgtatactcg aaattcgaca
      301 acacagaaat ttttaatttg gctgtgaatc cgtatcgtct aacttgtaga gctggttcta
      361 tagtagttga tatatcaaca ctaggctttt tggcttcaag gaaaaattta cgctcagaga
      421 agagtctaaa cgtgtagtct ggctttttag tattttcgcc tgctcgggct tttaaacctt
      481 cctctacaag cacttcgcgt tcgtttgttg gttttcctgc tgcgttcgtt atgtcccaac
      541 cgagaataag gaatagttga tctaaaaaat ccgttcttag ttgagtttcg ttgtatctgg
      601 cagaacgata ataatcaata tcgcttt
//
LOCUS       DAPHCV010000423          588 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.429, whole genome shotgun sequence.
ACCESSION   DAPHCV010000423 DAPHCV010000000
VERSION     DAPHCV010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.429"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(60..587)
                     /locus_tag="RH033_005078"
     CDS             complement(60..587)
                     /locus_tag="RH033_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2994100.1"
                     /translation="MAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFEAGGDS
                     VSINMPTVPTSVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDKITGND
                     KAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQ
                     SGDVLSSLVMRVAQD"
BASE COUNT          176 a          132 c          108 g          172 t
ORIGIN      
        1 aaggccgcca tgggatagcg gccatgttta ttacctggag aaacttacaa ttttatttat
       61 taatcctgcg ccacacgcat aaccagagaa gataacacat cacctgattg cactgagtta
      121 tctgtcagtt tcaggtattt tgctttaaac ggaatagcat attgaccttc cgctgtttta
      181 taactctcaa tgttaaaagg tgcagaaaca tccaggacat cgctgttatc acctaaatag
      241 agatagtaac ccacggtatc gttgctactg gctatcgcct tatcgttacc ggttatttta
      301 tcgttggtaa tcaacgagag ttttacacca gcaacttctt tcgggcagct aatttttaac
      361 atgctgctgg ttacaccgac tgcatcatca taggtagaat atttagcttt accttcaaag
      421 acactggttg gtacagtcgg catattaata cttactgaat caccacccgc ttcgaattta
      481 cacggagatt ctgcaatatt ccccttaata gttaatgttc cggtatcaac gtctgcagca
      541 aatactccag cgctcataaa aaaagaagaa aagacagcgg cagcaata
//
LOCUS       DAPHCV010000424          588 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.430, whole genome shotgun sequence.
ACCESSION   DAPHCV010000424 DAPHCV010000000
VERSION     DAPHCV010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.430"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..310
                     /locus_tag="RH033_005079"
     CDS             <1..310
                     /locus_tag="RH033_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDV2994101.1"
                     /translation="PECFSQLILAIRQCIHISLMTERWYPSLEPCRLIYYSGSWYLIA
                     LQKGKLQVFPLADIKSVSLTSERFERRGHIHSLVAEERFISALPHFSFIHKLINTFNL
                     "
BASE COUNT          147 a          163 c          128 g          150 t
ORIGIN      
        1 ccccgagtgt ttctcgcaac tcatcctggc aataagacag tgtatccaca tctctctgat
       61 gactgagcga tggtatccgt cactggagcc ctgccggctc atttattaca gcggtagctg
      121 gtatctgatc gcgttacaga agggaaaact gcaggtcttt cctctggcag atatcaaatc
      181 agtcagcctg acatcagaac ggtttgaacg gagaggccac atccacagtc tggtcgctga
      241 agagcgtttt atctccgccc tgccacattt ctctttcatc cataaactta tcaacacctt
      301 taacctgtga tcgccggcct gccaaagccg tcccgacagg tatggagaca atatgttgaa
      361 cagaaaacta aatatacggc tacgtcattc cctgaacagt cactgcatac cttccatcat
      421 tatcaataac accgtacgtt catttcagag gtcagtcatg aataccagag ctctttttcc
      481 cctgctgttc actgtggcat cattctccgc ctccgccggc aactgggctg tcaaaaacgg
      541 ctggtgtcag accatgacgg aagatggtca ggcgctggta atgctgaa
//
LOCUS       DAPHCV010000425          587 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.431, whole genome shotgun sequence.
ACCESSION   DAPHCV010000425 DAPHCV010000000
VERSION     DAPHCV010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.431"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>587
                     /locus_tag="RH033_005080"
     CDS             <1..>587
                     /locus_tag="RH033_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HDV2994102.1"
                     /translation="LFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGEQSGTV
                     NGVTPPAVQHLTAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTA
                     RTTETTYRFTQLALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSQIELTPGYFQI
                     TATPHLAVYDPTVQFEFWFSETRITDIRQVETTAR"
BASE COUNT          128 a          165 c          192 g          102 t
ORIGIN      
        1 ctgttccgct gcgtgagtat ccgtgagaac gacgacggca cgtatgccat caccgccgtg
       61 cagcatgtac cggaaaaaga ggccatcgtg gataacgggg cgcactttga cggcgaacag
      121 agtggcacgg tgaacggtgt cacgccgcca gcggtgcagc acctgaccgc cgaagtcact
      181 gcagacagcg gggaatatca ggtgctggcg cgatgggaca ctccgaaggt ggtgaagggc
      241 gtgagcttcc tgctccgtct gaccgtaaca gcggacgacg gcagtgagcg gctggtcagc
      301 acggcccgga cgacggaaac cacataccgc ttcacgcaac tggcgctggg gaactacagg
      361 ctgacagtcc gggcggtaaa tgcgtgggga cagcagggcg atccggcatc ggtatcgttc
      421 cggattgccg caccggcagc gccgtcacag attgagctga cgccgggcta ttttcagata
      481 actgccacgc cgcatcttgc ggtttatgat ccgacggtac agtttgagtt ctggttctcg
      541 gaaacgcgga ttaccgatat caggcaggtt gaaaccacag cccgcta
//
LOCUS       DAPHCV010000426          586 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.432, whole genome shotgun sequence.
ACCESSION   DAPHCV010000426 DAPHCV010000000
VERSION     DAPHCV010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.432"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..507
                     /locus_tag="RH033_005081"
     CDS             <1..507
                     /locus_tag="RH033_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rhs family protein"
                     /protein_id="HDV2994103.1"
                     /translation="QNLRYQGQYLDRETGLHYNLHRYYDPDVGRFIVTDPIGLAGGLN
                     LYAYAPNPLKYIDPLGLKKELCNGTTRVRHYTNRKGSNAIAESGIIKAHDNGRVYLEP
                     ANKKPLSQVDIEAKYQIGRGKGRDYVETDVPNEQLEWVTNPRYHIDELTVKGDIVLSD
                     NAKITRRK"
BASE COUNT          195 a           99 c          144 g          148 t
ORIGIN      
        1 cagaacctgc gctaccaggg acagtacctc gacagggaaa cagggctgca ttacaatctg
       61 cacaggtact atgatccgga tgtcgggcgg ttcattgtaa cggatccgat agggctggcg
      121 ggtgggctga acttatatgc ttatgcgcct aatccgctga aatatattga tccactcgga
      181 ttaaaaaaag agctctgtaa tggaactact cgtgtgcggc attatactaa tcgtaaaggt
      241 tcaaatgcga ttgctgaaag tgggataatt aaggcgcatg ataacggtcg tgtatatttg
      301 gaacctgcga ataaaaaacc actcagtcaa gtagacatag aggcgaaata tcaaataggt
      361 cgtgggaaag gtagagatta tgtggaaaca gatgtaccta acgagcaatt agagtgggta
      421 acaaaccctc gataccatat agatgaattg actgtgaaag gtgatattgt tctttctgat
      481 aatgcaaaaa taactcggcg taaataatat gaataaaaac aatgatgtaa ttaatatacc
      541 cgggaaggat gccctttata ttctaagtga ggtagagtac atattg
//
LOCUS       DAPHCV010000427          580 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.433, whole genome shotgun sequence.
ACCESSION   DAPHCV010000427 DAPHCV010000000
VERSION     DAPHCV010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.433"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..461)
                     /locus_tag="RH033_005082"
     CDS             complement(<1..461)
                     /locus_tag="RH033_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDV2994104.1"
                     /translation="MQLASRFGHVNQIRRERPLTREELMYHVPSIFGEDRHTSRSERY
                     AYIPTITVLENLQREGFQPFFACQTRVRDQSRREYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAY"
BASE COUNT          133 a          158 c          157 g          132 t
ORIGIN      
        1 tcgtaagccc cttcaatgac tctgtccacc acatttcccc ggtgcggaac acgcacttcc
       61 cccagagact ggccacagac gcacccgttc tggcagacga acctgaagta acccggcagc
      121 atctggtagc tggaggtacc gtcatgagag ttgagcagaa taatttcagg gacatgttct
      181 ccgtttatct ctccggcccg ccgcagacgc agcatgtgtt tggtatattc ccggcggctc
      241 tggtcgcgca cacgggtctg gcaggcgaag aacggctgaa agccttcccg ctgcagattt
      301 tccaggacgg taatggtggg aatgtacgca taccgttcac tgcgggaggt gtgccggtct
      361 tctccaaaaa tactcgggac gtggtacatc agttcttcgc gtgtcagtgg gcgctcccga
      421 cggatctgat ttacatgacc aaaacgactg gctaattgca tactttgctc cttatgaatg
      481 aattagcggt ataaacgaaa aagccgcgtc tcccggagaa gacgcggcct gatggatgaa
      541 atgaagacgt ttattgcctg agaattccct cactggcgag
//
LOCUS       DAPHCV010000428          569 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.434, whole genome shotgun sequence.
ACCESSION   DAPHCV010000428 DAPHCV010000000
VERSION     DAPHCV010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.434"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..531
                     /locus_tag="RH033_005083"
     CDS             <1..531
                     /locus_tag="RH033_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HDV2994105.1"
                     /translation="IADLVYAILTSNPKISTDNVSLFDKAKHANVLESAAMDVASLDK
                     ARQLMRVQKEGERHLNIRPAFVLVPTAMESVANQVIRSSSVKGADINAGIINPVKDFA
                     TVIAEPRLDDNSQTTFYLAASKGSDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRID
                     AGVAPVDHRGLVKCTA"
BASE COUNT          139 a          134 c          158 g          138 t
ORIGIN      
        1 attgccgatc tggtttatgc cattctgacg tctaacccga aaatctccac agataatgta
       61 agtctgttcg ataaagcgaa acatgcaaac gtactggaga gcgcggcaat ggacgtggca
      121 tcgctggata aagcccgcca gttgatgcgc gttcagaaag agggtgagcg tcatctgaat
      181 attcgtcctg cgttcgtact ggtaccgacg gcgatggagt ctgttgctaa ccaggtcatt
      241 cgctcctcaa gtgtcaaggg ggctgacatt aacgccggta ttattaaccc ggtgaaagat
      301 tttgcgaccg ttattgcaga gcctcgtctt gatgataaca gccagaccac cttctacctg
      361 gctgcgtcca aaggctccga tacgattgaa gtggcttatc tcaacggtgt ggatacgcca
      421 tatattgatc agatggaggg cttcagtgtg gatggcgtga caacgaaagt gcgtattgac
      481 gccggtgtcg cgccagttga tcaccgcggt ctggtgaaat gtacggcgta aacgtcgcag
      541 acaacaactc tgatggcccg taagggctt
//
LOCUS       DAPHCV010000429          568 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.435, whole genome shotgun sequence.
ACCESSION   DAPHCV010000429 DAPHCV010000000
VERSION     DAPHCV010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.435"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(79..>568)
                     /locus_tag="RH033_005084"
     CDS             complement(79..>568)
                     /locus_tag="RH033_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2994106.1"
                     /translation="ADGTQVSFPYAGEWLTEDEIRAVLDAVHDAVRSVSCRVAEDARR
                     IRAALTTTGQTLLTRQTRRFRLVVKESDHPCWLDEDDENLPVVLDAILNRGARFSSVE
                     MYLVSECVEHILSSGLACDVLRIPDEPSRRWFDRDILREVVLEARTEIRSMADALAKI
                     RK"
BASE COUNT          113 a          190 c          151 g          114 t
ORIGIN      
        1 gcggagcctg cggcgacatc cttttgctct ttccggggac cggctggaaa aggcggagat
       61 ttttccccgc cagcgtcatc atttccggat tttcgccagt gcatccgcca tgctgcggat
      121 ttcagtccgg gcttccagga ccacctcccg cagaatatcc cggtcaaacc agcgacggga
      181 cggttcatca ggtatacgca gcacatcgca ggccagccca ctggacagga tatgctccac
      241 acactcactg accagataca tttccaccga cgaaaaacga gcgccccggt tcaggatggc
      301 atcgagcacc acgggcaggt tttcgtcgtc ttcatcgagc cagcaggggt gatcgctttc
      361 cttcacaacc aggcgaaagc ggcgcgtctg gcgggtcagc aacgtctgac cggttgtggt
      421 cagcgccgcc ctgatacgcc gcgcatcttc tgccacccgg caactgacac tgcgtaccgc
      481 atcgtgcacc gcatcaagaa ctgccctgat ttcgtcttcg gtcagccatt ctccggcata
      541 cgggaaagac acctgcgttc cgtctgcc
//
LOCUS       DAPHCV010000430          564 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.436, whole genome shotgun sequence.
ACCESSION   DAPHCV010000430 DAPHCV010000000
VERSION     DAPHCV010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.436"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..459
                     /locus_tag="RH033_005085"
     CDS             <1..459
                     /locus_tag="RH033_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="RepA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HDV2994107.1"
                     /translation="TEYDPLIGCYIPTDITFTLALFAALDVSEDAVAAARRSRVEWEN
                     KQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIKRARAHRDANRERQDIVT
                     LVKRQLTREISEGRFTANGEAVKREVERRVKERMILSRNRNYSRLATASP"
BASE COUNT          144 a          147 c          159 g          114 t
ORIGIN      
        1 acggaatatg acccgcttat cgggtgctac attccgaccg acatcacgtt cacactggct
       61 ctgtttgctg cccttgatgt gtctgaggat gcagtggcag ctgcgcgccg cagtcgtgtt
      121 gaatgggaaa acaaacagcg caaaaagcag gggctggata ccctgggtat ggatgagctg
      181 atagcgaaag cctggcgttt tgtgcgtgag cgtttccgca gttaccagac agagcttaag
      241 tcccgtggaa taaaacgtgc ccgtgcgcat cgtgatgcga acagggaacg tcaggatatc
      301 gtcaccctgg tgaaacggca gctgacgcgt gaaatctcgg aaggacgctt cactgctaat
      361 ggtgaggcgg taaaacgcga agtggagcgt cgtgtgaagg agcgcatgat tctgtcacgt
      421 aaccgtaatt acagccggct ggccacagct tctccctgaa agtgatctcc tcagaataat
      481 ccggcccgcg ccggaggcat tcgcacgcct gaagcccgtc agtgcacaat aaatcagcac
      541 tacatacaaa aaacaacctc acca
//
LOCUS       DAPHCV010000431          562 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.437, whole genome shotgun sequence.
ACCESSION   DAPHCV010000431 DAPHCV010000000
VERSION     DAPHCV010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.437"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>562
                     /locus_tag="RH033_005086"
     CDS             <1..>562
                     /locus_tag="RH033_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2994108.1"
                     /translation="LTLFSGNVIAATVELGFENEQYNYAYRSADVFMPYIKSNFNPVT
                     DSALNVSLTYMYQDQYGKKHKKTSEDRFKTNRDRIELYLKGYTLNRGAYSFSPSAGFR
                     YESWDVNYDNPKKQDKWKLELRFYPNMTYKLNDQLSLYMNGFVAPVFFKTQQESRKDN
                     NYVKGKLGAKRYNNDYYQELQILGVR"
BASE COUNT          188 a           93 c          106 g          175 t
ORIGIN      
        1 tattaacgtt attctctggc aacgtaattg ctgcaactgt agaattaggt tttgaaaatg
       61 agcaatataa ttatgcttat cgttctgcag atgtcttcat gccgtatatt aagagtaatt
      121 tcaaccctgt tactgattct gctttgaatg tgtcactcac ctatatgtat caggatcaat
      181 atgggaaaaa acataaaaaa acatctgagg acagatttaa aaccaatcgc gatcgcatag
      241 agctctatct taaaggttat actttaaata ggggagcata ttctttttct ccttccgcag
      301 gtttccgtta tgagtcatgg gatgtaaact acgataatcc gaaaaagcag gataagtgga
      361 aactggaact acgcttttat cctaatatga cttataaact caatgaccag ttaagcctat
      421 atatgaatgg ttttgttgcc cctgtatttt ttaaaacaca acaagagtcg agaaaagata
      481 acaattatgt aaagggtaag ttaggggcga aacgttataa caacgattat tatcaggaac
      541 tccagattct gggtgtcaga ta
//
LOCUS       DAPHCV010000432          558 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.438, whole genome shotgun sequence.
ACCESSION   DAPHCV010000432 DAPHCV010000000
VERSION     DAPHCV010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.438"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..460
                     /locus_tag="RH033_005087"
     CDS             <1..460
                     /locus_tag="RH033_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV2994109.1"
                     /translation="RGDITAPQSCEINSLRPINFDFKEIPAADFSSVAGSTVTTHKIT
                     KTVTIECENLGILNTDDISTSFYATEPSTDNSMVVTSNPNVGIKIYDKNNKEINVNGG
                     ELPTDMGKSTVFGEKAGSVTFSAAPASLTGARPAPGTFTATATITIEIVR"
BASE COUNT          168 a          122 c          112 g          156 t
ORIGIN      
        1 tcgtggcgat atcactgcac cgcaaagctg tgaaattaac agtttaagac ccattaattt
       61 tgattttaaa gaaattcctg cggcagattt ttcttcggta gctggaagta ccgtgacaac
      121 gcataaaatt accaaaaccg tcaccattga gtgtgaaaat ttaggaatac taaatactga
      181 tgatatcagt acctcttttt atgctaccga acccagtact gacaactcaa tggtcgtgac
      241 atcaaacccg aacgttggga ttaaaattta tgataaaaat aataaggaaa tcaatgttaa
      301 cggtggtgaa ctgccgacag atatgggtaa atcaaccgtc tttggtgaaa aagccggtag
      361 cgtcactttt tctgctgctc ctgcaagcct tacgggggct cgccccgcgc caggaacatt
      421 taccgcgact gcaacgataa caattgagat tgtacgctaa acgaactgac agcggtgttc
      481 ctcacaccgt tgtcatctta cgcatttttg tccgcgagtt ttcactctgc atgataaaaa
      541 gatgacatta cttttctt
//
LOCUS       DAPHCV010000433          553 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.439, whole genome shotgun sequence.
ACCESSION   DAPHCV010000433 DAPHCV010000000
VERSION     DAPHCV010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.439"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>553
                     /locus_tag="RH033_005088"
     CDS             <1..>553
                     /locus_tag="RH033_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="cleaves origin of transfer and unwinds; oriT
                     relaxase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HDV2994110.1"
                     /translation="VAALDTRKSKQHVDPEVRMAEWMQTLKETGFDIRAYRDAADQRA
                     EIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPENGVIERA
                     RAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPR
                     TAGYSDAVSVLAQDRPSLAIVSGQ"
BASE COUNT          124 a          147 c          185 g           97 t
ORIGIN      
        1 gtggcggcac tggatacgcg aaaatccaaa cagcacgtcg atcctgaagt cagaatggct
       61 gaatggatgc agacgctgaa ggaaaccggg ttcgacatcc gggcatatcg tgacgcggcg
      121 gatcagcgtg cggagatccg cacgcaggcg cccgggcctg cttcacagga cgggccggat
      181 gtgcagcagg cggtgacaca ggcgattgcc ggattaagtg aacgaaaagt gcagttcacg
      241 tatacggacg tgctggccag gacggtcggc atactgccgc cggaaaatgg tgtgattgaa
      301 cgggcacgcg ccggtatcga tgaggccatc agccgtgagc agcttatccc cctcgaccgt
      361 gagaaggggc tgttcacatc aggaattcat gtgctcgatg agctgtccgt ccgggcactc
      421 agtcgtgaca tcatgaaaca gaaccgggtg accgtacatc cggagaaaag tgtcccccgg
      481 acggctggtt acagcgatgc ggtcagtgtt ctggcacagg accgtccgtc gctggccatt
      541 gtgtccgggc agg
//
LOCUS       DAPHCV010000434          550 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.440, whole genome shotgun sequence.
ACCESSION   DAPHCV010000434 DAPHCV010000000
VERSION     DAPHCV010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.440"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>550
                     /locus_tag="RH033_005089"
     CDS             <1..>550
                     /locus_tag="RH033_005089"
                     /inference="COORDINATES: protein motif:HMM:NF025072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDV2994111.1"
                     /translation="ECGYSQEDKKTFNILFLGNFDYNPNADAARFLCNEIYPSLQSMY
                     MDVKVYIVGKNPPNDLLDSKNIIRTGFVSDVSVYYRMATVFICPLKLGAGMKNKILEA
                     MATGLPIISSDVGVEGIDGLTDGENYLRANTLHEYVDAITRVHNNPLLGDGLGRKAAK
                     LISEKYDWQNIVDRYVTKLQDIS"
BASE COUNT          188 a           77 c          114 g          171 t
ORIGIN      
        1 agagtgtgga tattcacaag aagataaaaa aacatttaat atattatttt tagggaattt
       61 tgattataat cctaatgcgg acgccgcaag gtttttatgt aacgaaatat atccgtcgct
      121 acagtcaatg tatatggatg taaaagttta tattgtcggc aaaaatcctc ccaatgattt
      181 attggattca aaaaatatca tcaggactgg ttttgttagt gatgtatcag tgtattacag
      241 aatggctact gtttttatat gccctctgaa acttggcgca ggaatgaaaa ataaaatttt
      301 agaagctatg gcaacaggat tgccaattat atcatccgac gttggagttg aaggaattga
      361 tggacttact gatggtgaaa attacctgag agctaacaca ttgcatgagt atgtagatgc
      421 cattactaga gtgcataata atccattgct aggcgatggg cttggaagaa aagctgctaa
      481 gcttatttct gaaaaatatg attggcaaaa tattgtggac agatacgtta caaagctaca
      541 agatatttcc
//
LOCUS       DAPHCV010000435          549 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.441, whole genome shotgun sequence.
ACCESSION   DAPHCV010000435 DAPHCV010000000
VERSION     DAPHCV010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.441"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(182..529)
                     /locus_tag="RH033_005090"
     CDS             complement(182..529)
                     /locus_tag="RH033_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDV2994112.1"
                     /translation="MVSQMSFSGAILWIKIFSSVFLVPVYEEIVFRGCLFNSFKLWFH
                     DNIYIPAIVTSVIISALHLQYTYFRTFLMLFLVSLVLISAKIKSNGLLMPILLHMAMN
                     VAIAGIQYLAYIN"
BASE COUNT          203 a           85 c           91 g          170 t
ORIGIN      
        1 agtgtaatat caggcacata ttttgagaga gttaaatatt atattaatga agcgcctata
       61 gcaggtgctt cattatatca taagagatga tggctacctt gtgttgataa gatacagcat
      121 aataacgatt ccatttcatt tttgttgtag ggttgtttaa caaaaaatct tgctaacggg
      181 cttaattgat ataagccaga tattgtattc ctgctatggc tacattcatt gccatgtgaa
      241 gtaatatagg cattaatagg ccattcgact ttatttttgc actaataaga actaacgata
      301 ccagaaataa catcaaaaat gtacgaaagt atgtatattg tagatgcaat gcagagatta
      361 taactgatgt tacaattgca gggatataaa tattatcatg gaaccagagt ttaaaagagt
      421 taaaaagaca ccctcgaaat acaatctctt catatacagg aacaagaaat acagaagaaa
      481 atattttaat ccataaaatt gccccagaaa aagacatttg cgaaactatc cactcttccg
      541 ttttcctta
//
LOCUS       DAPHCV010000436          547 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.442, whole genome shotgun sequence.
ACCESSION   DAPHCV010000436 DAPHCV010000000
VERSION     DAPHCV010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.442"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            45..>547
                     /locus_tag="RH033_005091"
     CDS             45..>547
                     /locus_tag="RH033_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2994113.1"
                     /translation="MIRYSGIFLSLLMLSGCATEALKNEQAETVITAGNKAVAANASF
                     IKQYYASRENFLVNFYATNPDCALRPDYTIDIGGDGNGGKLCQRSGPNTKQVQLVPFE
                     KEKLLASMEVINSMSAYVELLTKDMATTESQQVALLKQALDHANNAQTLLGVLHSKDL
                     AQSAAAV"
BASE COUNT          180 a          101 c          118 g          148 t
ORIGIN      
        1 actatttaat tatctgagat aaactcaacg tcaagaggtg ggttatgatt agatatagtg
       61 gaatatttct atcgctgtta atgctttcag gttgtgcaac tgaagcattg aaaaacgaac
      121 aagctgaaac tgttattact gcgggtaaca aagcagtagc ggcgaatgca agtttcatta
      181 aacaatacta tgcttctcgt gaaaacttcc ttgttaattt ctacgcgact aatccggatt
      241 gtgccctgcg acctgattat accattgata taggcggtga tgggaatggc ggaaaattat
      301 gccagcgttc agggccaaat acaaaacaag tacaattagt ccctttcgag aaagaaaaat
      361 tactggcttc aatggaagtc attaatagca tgagcgctta tgtggaactt ctgacaaagg
      421 atatggcaac tacggaaagt cagcaagtgg cattacttaa acaggctctg gatcacgcaa
      481 acaacgcaca aacactactt ggtgttttac atagcaaaga tttagctcaa tctgcagcag
      541 cggttaa
//
LOCUS       DAPHCV010000437          541 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.443, whole genome shotgun sequence.
ACCESSION   DAPHCV010000437 DAPHCV010000000
VERSION     DAPHCV010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.443"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>541)
                     /locus_tag="RH033_005092"
     CDS             complement(<1..>541)
                     /locus_tag="RH033_005092"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2994114.1"
                     /translation="EILHTNIDAVSYQGGYYWWKDIDFNRKSYYEQAGAFWYPSATFE
                     PRLIRADDSLRALLSGGGLSVGLLPRLYHGIVRRFVLDNLKKKTGRYVHGASPDISLA
                     VSLALLGVNTFYISTPLTIFGASKKSGGGWTAENKHYGSIREQQHIPQYTKDNWSSFL
                     PDVWSEHTIYPQSIIEVYDS"
BASE COUNT          173 a          127 c           92 g          149 t
ORIGIN      
        1 actatcatag acctcaataa tagactgagg atatatggtg tgctcagacc atacatcagg
       61 caagaaacta gaccaattat ccttagtata ttgaggtata tgctgctgtt ctcttatact
      121 accatagtgc ttgttttcgg cagtccatcc accaccactt ttctttgagg cgccaaaaat
      181 tgttaatggt gtacttatat aaaatgtgtt aacacctaac aaagcaaggc ttaccgccaa
      241 cgatatatca ggcgaagctc cgtgaacata tctaccagtt ttctttttca aattatctaa
      301 aacaaacctt ctcacgattc cgtggtataa ccttggtagt agaccaactg aaagcccacc
      361 tccgctaagc agagcacgaa gtgaatcatc agcacgaatt aaccgaggct caaacgtagc
      421 agaaggatac caaaacgcgc cagcctgttc ataatagctt ttccgattaa agtcaatatc
      481 cttccaccaa taataaccgc cttggtatga tacagcatct atattagtat ggagaatctc
      541 t
//
LOCUS       DAPHCV010000438          536 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.444, whole genome shotgun sequence.
ACCESSION   DAPHCV010000438 DAPHCV010000000
VERSION     DAPHCV010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.444"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>536
                     /locus_tag="RH033_005093"
     CDS             <1..>536
                     /locus_tag="RH033_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDV2994115.1"
                     /translation="KEVPEIILLNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRV
                     PHKGDIVGQVIEGAYEVLGVFDKVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENK
                     TPVTPEQIITPRRWEDKQNDLWTTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGD
                     IRINKALWVIAEQFRKWK"
BASE COUNT          165 a           92 c          144 g          135 t
ORIGIN      
        1 gaaagaagtt cctgaaatta tcctgcttaa ttcacatgat ggttcatcca gttatcagat
       61 gatccccgga atttttcgtt ttgtctgcac aaatggcctg gtgtgcggaa ataattttgg
      121 cgaaatccgc gttccacata aaggtgatat tgtcgggcag gttatagagg gcgcgtatga
      181 agtgctcggt gtctttgata aggtcactga taatatggag gcgatgaaag aaattcatct
      241 taacagtgac gagcaacatt tatttggcag agctgcactg atggtcaggt atgaagatga
      301 aaataaaacg ccagtgacgc ctgaacaaat aattactccc cgtcgttggg aggataaaca
      361 gaacgatctc tggacaacct ggcagcgggt tcaggagaat atgataaaag gtggattatc
      421 ggggcgaagt gcctccggga aaaataccag gacaagagcc attacaggta ttgatggtga
      481 tataagaatc aacaaggcgt tgtgggtgat tgccgaacag ttcagaaaat ggaagt
//
LOCUS       DAPHCV010000439          535 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.445, whole genome shotgun sequence.
ACCESSION   DAPHCV010000439 DAPHCV010000000
VERSION     DAPHCV010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.445"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..224)
                     /locus_tag="RH033_005094"
     CDS             complement(<1..224)
                     /locus_tag="RH033_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HDV2994116.1"
                     /translation="MAVRISGVLKDGAGKPIQNCTIQLKARRNSTTVVVNTVASENPD
                     EAGRYTMDVEYGQYSVSLLVEGFPPSHAGT"
     gene            complement(289..>535)
                     /locus_tag="RH033_005095"
     CDS             complement(289..>535)
                     /locus_tag="RH033_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HDV2994117.1"
                     /translation="VAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAWGA
                     GVQFNPTESVAIDIAYEGSGSGDWRTNSFIVGVGYKF"
BASE COUNT          116 a          164 c          129 g          126 t
ORIGIN      
        1 atggtcccgg catgtgatgg cgggaatccc tccaccaaca gactgacgct gtactgacca
       61 tactcgacgt ccattgtgta acgcccggct tcatccgggt tttctgaggc cactgtgttc
      121 accaccaccg tggtgctgtt gcgcctggcc tttagctgaa tggtgcagtt ttgtatcggc
      181 ttacctgcgc catctttcag tacacctgaa atccgtactg ccatattccc cccacaaaaa
      241 agcccgcctg aaccggcggg ctgtcataac actgtgttac ctggctaatc agaatttata
      301 accgacacca acgatgaaac tgttggtacg ccagtcaccg ctgccggagc cttcataagc
      361 aatatcaatg gccacggatt cggtcgggtt aaactgcacg ccagctcccc acgccagaga
      421 cgtgttgctg tggcgaccgt catcacttcc ggtcagcacg tcgtgcgttt tccccttgtt
      481 gtcagttacg cggagataat ccccggagaa agtcgacaca cggctgtaag ccaca
//
LOCUS       DAPHCV010000440          506 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.446, whole genome shotgun sequence.
ACCESSION   DAPHCV010000440 DAPHCV010000000
VERSION     DAPHCV010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.446"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..271
                     /gene="traA"
                     /locus_tag="RH033_005096"
     CDS             <1..271
                     /gene="traA"
                     /locus_tag="RH033_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HDV2994118.1"
                     /translation="RAVIPAAVLMMFFPQLAMAAGSSGQDLMASGNTTVKATFGKDSS
                     VVKWVVLAEVLVGAVMYMMTKNVKFLAGFAIISVFIAVGMAVVGL"
     gene            286..>506
                     /gene="traL"
                     /locus_tag="RH033_005097"
     CDS             286..>506
                     /gene="traL"
                     /locus_tag="RH033_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HDV2994119.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLR"
BASE COUNT          115 a          113 c          145 g          133 t
ORIGIN      
        1 tcgcgcagtg atcccggctg ctgttctgat gatgttcttc ccgcagctgg cgatggccgc
       61 cggcagcagt ggtcaggacc tgatggcaag cggtaacacc acggttaagg cgaccttcgg
      121 taaggactcc agtgttgtta aatgggttgt tctggctgaa gttctggtcg gtgctgtcat
      181 gtacatgatg accaaaaacg tcaagttcct ggccggtttt gccatcatct ctgtatttat
      241 tgctgtgggt atggccgtcg ttggcctctg acaggaaata aaacgatgtc gggagacgag
      301 aataaactta agaaatatcg tttcccggaa acactgacca accagagccg ctggtttggc
      361 ctgccactgg atgaactgat ccccgcagca atctgtattg gctggggtat cacaacatcg
      421 aaatatctgt tcggtattgg tgcagcggtt ctggtttatt tcgggattaa aaaactgaaa
      481 aaagggcggg gcagttcctg gttacg
//
LOCUS       DAPHCV010000441          501 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.447, whole genome shotgun sequence.
ACCESSION   DAPHCV010000441 DAPHCV010000000
VERSION     DAPHCV010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.447"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..159)
                     /gene="ydfK"
                     /locus_tag="RH033_005098"
     CDS             complement(<1..159)
                     /gene="ydfK"
                     /locus_tag="RH033_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HDV2994120.1"
                     /translation="MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYI
                     EGNIKKKSW"
     gene            complement(228..341)
                     /locus_tag="RH033_005099"
     CDS             complement(228..341)
                     /locus_tag="RH033_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2994121.1"
                     /translation="MNSILIITSLLIIFSIFSHALIKLGIGISNNPDKTDV"
BASE COUNT          136 a           79 c          104 g          182 t
ORIGIN      
        1 ccatgacttt ttctttatgt ttccttcaat gtaatcaagc aatgttctgg tattgatagg
       61 tcttccctgt tttgctactt ccactacagc atcccctagg ataattctta cttcaggaag
      121 ctgcgcaggg aaccacttta gggtgtcttt tgatttcatg aagatattcc ttaaaatatt
      181 attgattttc attgcgatat tgtatgtctg attcaggata tgttgactta tacatcggtt
      241 ttgtctgggt tattggatat gccaatccct aattttatta gagcatgact aaaaatgctg
      301 aatatgataa ggagcgaagt gattatcagt atgctgttca tatagcctcg aattagtaat
      361 gtgttatata tgatatagtt gacaattttt atcttgggtg ttcttaaagt tcgtagataa
      421 acattgtcgt ttcaggtata caggaatgct aacaggtggc ggcaaaaatc aggcggttta
      481 tggcgcaagc tgaagtggca a
//
LOCUS       DAPHCV010000442          486 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.448, whole genome shotgun sequence.
ACCESSION   DAPHCV010000442 DAPHCV010000000
VERSION     DAPHCV010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.448"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>486
                     /locus_tag="RH033_005100"
     CDS             <1..>486
                     /locus_tag="RH033_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine methyltransferase"
                     /protein_id="HDV2994122.1"
                     /translation="EDRDLEEYETLYHFAQDKDFQSLVKKLKSSDKKYNSGLFSLEYI
                     DELLSNANSCIWSIIEQLYFPQSTYSFSVFSSDILGNIYEIFLSEKVRIDELGNVKIQ
                     PKEEHIDRDVVTTPTHIVKEIIRNTVVEYCKGKSDIEILNSKFADIACGSGAFIIEAF
                     QF"
BASE COUNT          171 a           76 c           83 g          156 t
ORIGIN      
        1 gaagatagag acttggaaga atacgaaact ctttatcact ttgcacaaga taaggatttt
       61 caatctttag tcaaaaaatt gaaatcctca gacaagaaat ataattcagg attattctcg
      121 ctagaatata ttgatgagtt attaagcaat gctaactctt gcatttggag catcatagaa
      181 caattatact tcccacaaag cacatactct ttctctgttt tctcttctga tattttaggt
      241 aatatatatg aaatattcct gagtgagaaa gttcgtattg atgaacttgg aaacgttaag
      301 attcaaccga aagaggaaca tatcgataga gacgttgtta ctacaccaac tcacattgta
      361 aaagaaatca ttagaaatac tgtcgtagaa tattgcaaag gcaagtcaga tattgagata
      421 ttgaattcaa aatttgctga tatcgcttgt ggctcaggtg cttttattat tgaggcattc
      481 caattc
//
LOCUS       DAPHCV010000443          486 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.449, whole genome shotgun sequence.
ACCESSION   DAPHCV010000443 DAPHCV010000000
VERSION     DAPHCV010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.449"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..362)
                     /gene="tnpA"
                     /locus_tag="RH033_005101"
     CDS             complement(<1..362)
                     /gene="tnpA"
                     /locus_tag="RH033_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDV2994123.1"
                     /translation="MGNEKSLAHTRWNCKYHIVFAPKYRRQVFYREKRRAIGSILRKL
                     CEWKSVRILEAECCADHIHMLVEIPPKMSVSGFMGYLKGKSSLMLYEQFGDLKFKYRN
                     REFWCRGYYVDTVGKNTA"
BASE COUNT          117 a          132 c           87 g          150 t
ORIGIN      
        1 ttcgccgtgt tcttacccac cgtatcgacg tagtaccctc tgcaccagaa ctctctgttc
       61 ctgtatttga atttcaaatc accaaactgc tcgtaaagca tcagactgct tttccctttc
      121 agatatccca taaagccgga tacactcatt ttgggcggga tctccacaag catatggata
      181 tgatctgcac agcattcagc ttcaagaatc cgtacgcttt tccactcaca cagctttctc
      241 aaaatactgc ctattgctct acgcttctct ctgtagaaca cttgtcttcg gtattttggc
      301 gcaaaaacta tgtgatattt acagttccat cgggtgtgcg ctaagctctt ttcgttcccc
      361 attgggaccc ccttttgatt tcttgtttga cacttgcagt tgccagaccg caaggtgttt
      421 taacaaatca aaaggggttt taataactgg ctcaaagctg aaagctttcc ggaaccccca
      481 gcctag
//
LOCUS       DAPHCV010000444          482 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.450, whole genome shotgun sequence.
ACCESSION   DAPHCV010000444 DAPHCV010000000
VERSION     DAPHCV010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.450"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..>482
                     /locus_tag="RH033_005102"
     CDS             1..>482
                     /locus_tag="RH033_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDV2994124.1"
                     /translation="MLPIGLQAETSHSSMAKDTITVVATGNQNTVFETPSMVSVVTND
                     TPWSQNAVTSAGMLKGVAGLSQTGAGRTNGQTFNLRGYDKSGVLVLVDGIRQLSDMAK
                     SSGTYLDPALVKRIEVVRGPNSSLYGSGGLGGVVDFRTADAADFLPPGETNGLSLWGN
                     "
BASE COUNT          124 a          117 c          135 g          106 t
ORIGIN      
        1 atactcccga tagggttaca ggcagagacc agtcattctt ctatggctaa agatacaatc
       61 accgttgtcg cgacaggaaa tcagaacacg gtatttgaaa cgccgtcaat ggtcagtgtc
      121 gtcacgaatg acacgccatg gagtcagaat gcggtcacat cagccggcat gctgaaaggt
      181 gttgccggtc tcagccagac tggtgcagga cggaccaatg ggcagacctt taatctacgc
      241 ggctatgaca aaagcggggt acttgttctt gttgacggca tccgtcaact cagtgacatg
      301 gcaaaaagca gtggcactta tctggatccg gcactcgtca aacgtatcga agttgtccgc
      361 ggaccaaact ccagtctgta cggcagtggc gggctgggag gtgttgtgga cttcagaact
      421 gccgatgcag cagatttcct tccccccgga gagacaaacg gtttaagtct gtggggaaat
      481 at
//
LOCUS       DAPHCV010000445          481 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.451, whole genome shotgun sequence.
ACCESSION   DAPHCV010000445 DAPHCV010000000
VERSION     DAPHCV010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.451"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..239)
                     /locus_tag="RH033_005103"
     CDS             complement(<1..239)
                     /locus_tag="RH033_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HDV2994125.1"
                     /translation="MNTVLSVQGASAPVKKKSFFSKFTRLNMLRLARAVIPAAVLMMF
                     FPQLALAAQGAGQDLMQSGNSTVKATFGKDSSVVK"
     gene            complement(272..454)
                     /gene="traY"
                     /locus_tag="RH033_005104"
     CDS             complement(272..454)
                     /gene="traY"
                     /locus_tag="RH033_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HDV2994126.1"
                     /translation="MVLDDATNHKLLGARERSGRTKTNEVLVRLRDHLNRFPDFYNLD
                     AIKEGAEETDSIIKDL"
BASE COUNT          138 a          121 c           99 g          123 t
ORIGIN      
        1 catttaacaa cactggagtc cttaccgaag gtcgccttaa ccgtggagtt accactctgc
       61 atcaggtcct gacctgcccc ctgggcggcc agtgccagtt gcgggaagaa catcatcaga
      121 acagcagcag ggatcacagc gcgagccagg cgaagcatat tcagacgagt gaatttagaa
      181 aaaaacgatt tctttttgac gggcgcagaa gcaccctgaa cacttaaaac agtattcata
      241 agttacctct attgtggata gattaataaa cctaaagatc tttaattatc gaatcagttt
      301 cctctgctcc ctcctttatt gcatccagat tataaaagtc aggaaaacgg ttcagatgat
      361 cacgtaacct gactagcacc tcattagttt ttgttctccc actacgttcc cgggcaccta
      421 agagcttgtg attagtagca tcgtccaata ccagtaaaac tttattgccc ggagcaggcc
      481 t
//
LOCUS       DAPHCV010000446          480 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.452, whole genome shotgun sequence.
ACCESSION   DAPHCV010000446 DAPHCV010000000
VERSION     DAPHCV010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.452"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(27..>480)
                     /gene="traX"
                     /locus_tag="RH033_005105"
     CDS             complement(27..>480)
                     /gene="traX"
                     /locus_tag="RH033_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HDV2994127.1"
                     /translation="IAQFAYYLAGFPWYEGNILFSFAVAAQVLTWCETRTWWRSAEAM
                     LLLAMWLPFSGTSYGIAGLLMLAVSHRLYRAEDRMERLALVACLLAVIPALNLATSDA
                     AAVAGLVMTVLTVGLVSCTGKSLPRFWYGDFFPTFYACHLTVLGVLAV"
BASE COUNT          136 a          152 c          123 g           69 t
ORIGIN      
        1 cagaatcagc gccagcatca taccagtcac acagccagaa cgcccagcac agtaagatga
       61 caggcataga acgtcgggaa aaaatccccg taccagaaac ggggtagtga tttccctgta
      121 caggacacca gaccaacagt cagcaccgtc atcacaaggc cggccaccgc cgctgcatca
      181 ctggtggcaa ggttaagcgc cggaataaca gccagcaggc aggcgaccag cgccagacgt
      241 tccattctgt cttccgcccg gtacagcctg tggctgactg ccagcatcag cagcccggca
      301 atgccgtaac tggtgccgga gaaaggcaac cacattgcca gaagcaacat ggcctctgcg
      361 gaacgccacc aggtgcgcgt ttcacaccat gtcagcacct gtgccgcaac tgcaaaggaa
      421 aacaggatat tcccctcata ccagggaaag ccggccagat aatatgcgaa ctgggcaatc
//
LOCUS       DAPHCV010000447          471 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.453, whole genome shotgun sequence.
ACCESSION   DAPHCV010000447 DAPHCV010000000
VERSION     DAPHCV010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.453"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>471
                     /locus_tag="RH033_005106"
     CDS             <1..>471
                     /locus_tag="RH033_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV2994128.1"
                     /translation="DTIAVIEAPGASNARINGLGGGETNYFGYALASYLEPYRENRIS
                     MDPLSLPSDISLDFTSTSVIPTKGAFIPAKFKTNRGKKVLLNLEDEYGNPIPFGAIVR
                     VVSDSDNMSIVGDDGVVYLTAMKDTGVIEVVWGDRNNQKSRKIGYSLNDTPTPAG"
BASE COUNT          150 a           82 c          110 g          129 t
ORIGIN      
        1 gatactattg cagtgataga agctccagga gcatcaaatg caagaataaa tggtttaggt
       61 ggtggtgaga caaattactt tggttatgca ttggctagtt atttggaacc ataccgagaa
      121 aatagaatat caatggaccc gttatcttta ccctctgaca tctcactgga ttttaccagt
      181 acatcagtta ttccgacaaa gggggctttc ataccagcaa aattcaaaac aaatagagga
      241 aaaaaggttt tgctcaacct tgaagatgaa tatggaaacc ccattccatt tggtgcgatc
      301 gttcgagtcg ttagtgatag tgacaatatg agtattgtcg gtgatgatgg agttgtatat
      361 ctaactgcaa tgaaagacac tggcgttatt gaggttgtct ggggagatag aaataaccag
      421 aaaagccgaa agattggcta tagcctgaat gatactccca cccctgctgg g
//
LOCUS       DAPHCV010000448          466 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.454, whole genome shotgun sequence.
ACCESSION   DAPHCV010000448 DAPHCV010000000
VERSION     DAPHCV010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.454"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            94..450
                     /locus_tag="RH033_005107"
     CDS             94..450
                     /locus_tag="RH033_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDV2994129.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
BASE COUNT          116 a           93 c           99 g          158 t
ORIGIN      
        1 aacctttata acatccgaaa aagaaagata cgaatagcat ttacacaata cccggaatag
       61 actttcgtca cttttaatta aagggatgtt tttatgcagt ggtatttagc cgtattaaaa
      121 aattatgttg gtttctctgg tcgcgcacgt cgtaaagagt actggatgtt tactctgatt
      181 aacgccatcg tcggcgctat tatcaatgtc attcaattga ttttaggttt agagtttcca
      241 ttcctttctc tcatttacct ggcagcaaca atcattccgg tcattgctct gtgcgttcgt
      301 cgtctgcacg atactgaccg ttcaggtgca tgggcgcttc tctatctggt tccgatcatt
      361 ggctggttag ttctgtttgt atttgcctgt ttagaaggca actctggcag caatcgttac
      421 ggaaacgatc cgaagtttgg ttcaaattaa tcatagaatt ggggcg
//
LOCUS       DAPHCV010000449          460 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.455, whole genome shotgun sequence.
ACCESSION   DAPHCV010000449 DAPHCV010000000
VERSION     DAPHCV010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.455"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(167..358)
                     /locus_tag="RH033_005108"
     CDS             complement(167..358)
                     /locus_tag="RH033_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HDV2994130.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QRKKEAQQSTLSVESPVQR"
BASE COUNT          140 a           85 c          119 g          116 t
ORIGIN      
        1 tgtattaatg atgtatttat ttcgcagtcg aacaggataa acatcagtca ggacaaaaac
       61 gtaaaattta agatgaaaaa atatctgccg ctttgataat cccaaagagc aaaagcatcc
      121 gacagcgagg tgccgttatg ttctgtgaga gtggagaagg taaccgctaa cgttgtactg
      181 gtgattcgac ggagagtgtg gattgctgag cttctttttt gcgctgatgg tgctgccagg
      241 caccgacgga cgagtagata aaacggccaa agaagaagat aaagctgatg agcagtacga
      301 tacgggtcat gcgactgtta aatcggtgtc gttttcgcat actggttgcc tgactcacaa
      361 aaggttcctt gaagtatgtc ccacgccgtg gacggtactt acattaaggc acaacaggac
      421 aaaatggtca attcttcgtt atgtaaaaaa gcgtcagtgg
//
LOCUS       DAPHCV010000450          455 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.456, whole genome shotgun sequence.
ACCESSION   DAPHCV010000450 DAPHCV010000000
VERSION     DAPHCV010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.456"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            26..409
                     /locus_tag="RH033_005109"
     CDS             26..409
                     /locus_tag="RH033_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome protein TraM"
                     /protein_id="HDV2994131.1"
                     /translation="MARVNLYISNEVHEKINMIVEKRRQEGARDKDISLSGTASMLLE
                     LGLRVYDAQMERKESAFNQTEFNKLLLECVVKTQSTVAKILGIESLSPHVSGNPKFEY
                     ASMVDDIREKVSVEMDRFFPKNDDE"
BASE COUNT          151 a           64 c          105 g          135 t
ORIGIN      
        1 ttcagaatct aaaaggtact gtcttatggc cagagtaaat ctgtatatca gtaatgaggt
       61 tcatgaaaaa attaacatga ttgttgaaaa gcgtcgtcag gagggagcaa gagataaaga
      121 tataagcctt tcaggaactg cttcaatgct tcttgaatta gggctccgtg tatatgacgc
      181 tcagatggag cgtaaagagt ctgcatttaa ccagacagag tttaataaac ttcttcttga
      241 atgtgttgta aaaacacagt caacggtggc aaagatttta ggtattgaat ctctcagtcc
      301 tcatgtatcc ggaaacccga agtttgaata tgccagtatg gttgacgata tcagagagaa
      361 agtgtctgtt gagatggacc ggtttttccc aaaaaatgat gatgaataaa tgaaaatgaa
      421 gtttgagttg gttcaaatat aggagttttt atgat
//
LOCUS       DAPHCV010000451          453 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.457, whole genome shotgun sequence.
ACCESSION   DAPHCV010000451 DAPHCV010000000
VERSION     DAPHCV010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.457"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>453)
                     /gene="rfbC"
                     /locus_tag="RH033_005110"
     CDS             complement(<1..>453)
                     /gene="rfbC"
                     /locus_tag="RH033_005110"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDV2994132.1"
                     /translation="FMESWNQKVFNEVIGKTIFVQDNHSKSYKGTLRGLHFQTSNPQG
                     KLVRVINGEVFDVVVDLRHQSKTFGKWVGVYLSSVNNYQLWIPEGFAHGFYVVSDHAE
                     FVYKCTDYYNPYSQQTLSWDDPELGILWPLDTAPILSEKDKSGSSFKN"
BASE COUNT          148 a          104 c           59 g          142 t
ORIGIN      
        1 aaatttttaa agctactacc tgacttatcc ttctccgata aaattggagc agtgtctaat
       61 ggccatagaa tacccaactc aggatcatcc catgacagtg tctgttgcga gtaaggatta
      121 taatagtctg tacatttata aacaaactcc gcatggtcac tcactacata gaaaccatgt
      181 gcaaaccctt caggaatcca aagttgataa ttattcacag acgataaata aactcctacc
      241 cattttccaa aagtctttga ctgatgtctc aaatcaacaa caacatcaaa cacttctcca
      301 tttattactc ttactaattt tccttgcgga ttactggttt gaaagtgtaa ccccctaaga
      361 gtacctttat atgatttcga gtgattgtcc tgcacaaaga ttgttttccc aattacttca
      421 ttaaaaactt tctgattcca actctccata aaa
//
LOCUS       DAPHCV010000452          448 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.458, whole genome shotgun sequence.
ACCESSION   DAPHCV010000452 DAPHCV010000000
VERSION     DAPHCV010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.458"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            36..>448
                     /locus_tag="RH033_005111"
     CDS             36..>448
                     /locus_tag="RH033_005111"
                     /inference="COORDINATES: protein motif:HMM:NF014879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HDV2994133.1"
                     /translation="MPIDTEAVSLEDQVKARCWAREFKLDAIFSTDGDGDRPLVADNN
                     GQWIKGDVLGFLCSVALDVDAIVVPINCNSMIGKVGTLISVTRTRIGSPYVLDGINRL
                     KKTGNIVAGFEANGGYILSSTICKNGRVLEHLATR"
BASE COUNT          129 a           58 c          115 g          146 t
ORIGIN      
        1 cagaaactat ttctcttgga agaagcgatt gcttcgtgcc cattgatact gaagcagtga
       61 gtttagaaga ccaggttaaa gccagatgtt gggcgcgtga gtttaaatta gatgctattt
      121 tttctacgga tggtgatgga gataggcctc tggttgctga caacaatggg caatggatta
      181 aaggggatgt tttaggcttt ttatgttctg ttgctcttga tgttgatgcg attgtagtgc
      241 ctataaattg taattcaatg attggtaaag tgggtactct gatttcagta actagaacta
      301 gaattggctc accttatgta ctggatggta ttaatagatt aaaaaaaaca gggaatatag
      361 tcgctggatt tgaggctaat ggggggtata ttttatcatc gacaatatgt aagaatggaa
      421 gagttcttga acatttagca actcgtga
//
LOCUS       DAPHCV010000453          437 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.459, whole genome shotgun sequence.
ACCESSION   DAPHCV010000453 DAPHCV010000000
VERSION     DAPHCV010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.459"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..220)
                     /locus_tag="RH033_005112"
     CDS             complement(<1..220)
                     /locus_tag="RH033_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDV2994134.1"
                     /translation="MATTCSVILILESFDVYFGKESVFLERGSSVLVDSSSRDFFLTY
                     PERVIVADFGAEFISRYLKANNLRDISDC"
BASE COUNT          179 a           91 c           58 g          109 t
ORIGIN      
        1 tacaatcaga aatatccctt aagttattag ctttcaaata gcgactaata aactcagcgc
       61 caaaatccgc cactatcact ctttcaggat atgtcaggaa aaaatctcta gagctagagt
      121 cgacaagtac agatgaacct ctctccagaa acacactctc ttttccgaaa taaacatcaa
      181 aggactccaa aatcaatata actgaacatg tagttgccat atcatccacc caatttaact
      241 gaaacctgga tgaagtatat gcatataaag ttcactttgc caaacttcag tataaaaaac
      301 cacataaaaa atagggtgtg ataaaaaata ccgtaacaat aaagcaaagg attataaaat
      361 ccgttacagt tacaaatgat actcaagaaa caattcctta ggaaaaactt atttacagcc
      421 aataagggta atgactc
//
LOCUS       DAPHCV010000454          420 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.460, whole genome shotgun sequence.
ACCESSION   DAPHCV010000454 DAPHCV010000000
VERSION     DAPHCV010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.460"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            185..403
                     /locus_tag="RH033_005113"
     CDS             185..403
                     /locus_tag="RH033_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HDV2994135.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
BASE COUNT          142 a           67 c           97 g          114 t
ORIGIN      
        1 aattcatttt gggcgataac taaaccattt ttgagtaaca aatgaaacaa ctgcaacgga
       61 cttattattt ctggaaaacg cctcgcaaaa acaaaacaac atgcaaaaat ccgctttcgt
      121 taattaattt tccggcgtaa ttttcgcaca tacattaatg agtaatatat gtaaggtatt
      181 ggtaatggcg atgaatacgg tttttcttca tttatcagaa gaggcaatta aacggctgaa
      241 caagcttagg ggctggcgta aggtttcacg atctgcaatt ttacgcgaag cggtagagca
      301 atatctggaa cgtcagcaat tcccggtgcg taaagcaaag ggaggcaggc aaaaggggga
      361 ggtagttggt gttgatgatc agtgtaagga gcataaggaa tgacaaaaaa tttattattc
//
LOCUS       DAPHCV010000455          419 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.461, whole genome shotgun sequence.
ACCESSION   DAPHCV010000455 DAPHCV010000000
VERSION     DAPHCV010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.461"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..102
                     /locus_tag="RH033_005114"
     CDS             <1..102
                     /locus_tag="RH033_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate transporter"
                     /protein_id="HDV2994136.1"
                     /translation="EESNGHRVSLPLSVFNPQDYHPLLITVSGKNVN"
     gene            181..414
                     /locus_tag="RH033_005115"
     CDS             181..414
                     /locus_tag="RH033_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HDV2994137.1"
                     /translation="MPGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIR
                     NAEGQRRWRCRNSEPDAGKVLNTRLASDGLLRQ"
BASE COUNT          100 a          108 c          107 g          104 t
ORIGIN      
        1 gaagaaagta acggccatcg tgtcagtctg ccgttatcgg ttttcaatcc ccaggactac
       61 catccactgc tgattaccgt ttccggaaaa aacgttaact gatccctgaa gtcagtttca
      121 tgcttcagcc tctgtcacaa cgcgtggcag aggcttttta tttatcagga gaaaacagat
      181 atgccgggtt acacagagta tgtcctggct gaaggatctt tctcatacgg gcaggcggtt
      241 gctgtcataa ccgcttaccg caatgtgttt atccaggatg acccgggaat gcatttccgt
      301 cgggttatcc gcaacgcgga aggtcagcga cgctggcgct gccggaattc tgaacccgat
      361 gccggaaagg tgcttaatac gcggctcgcc agtgacggtc ttctcagaca ataaacgtc
//
LOCUS       DAPHCV010000456          417 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.462, whole genome shotgun sequence.
ACCESSION   DAPHCV010000456 DAPHCV010000000
VERSION     DAPHCV010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.462"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            48..371
                     /gene="iraM"
                     /locus_tag="RH033_005116"
     CDS             48..371
                     /gene="iraM"
                     /locus_tag="RH033_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HDV2994138.1"
                     /translation="MKWIVIDTLIQPTCGISFSAIWGDMKMIIWYQSTIFLPPGSIFT
                     PVKSGIILKDKEYPITIYNITPFNKDLWSLLKSSQECPPGESEITNKCLHNSCIIKIC
                     PYGLK"
BASE COUNT          154 a           72 c           63 g          128 t
ORIGIN      
        1 aacatcctaa tgaactatat taaataccgt gggataagac ataacaaatg aagtggatag
       61 taattgacac gttaattcaa cctacatgcg gcatatcttt ttcagccata tggggtgata
      121 tgaaaatgat catctggtat caatctacta tatttctccc tcctggaagt atatttacac
      181 cggttaagtc tggtattatc cttaaggata aagaataccc tattactatt tataacatca
      241 caccattcaa caaggattta tggagtttac tcaaaagcag tcaagagtgt cctccaggag
      301 aaagcgaaat aacaaataaa tgtttacata atagttgtat tataaaaata tgcccatatg
      361 gactcaagta atggtcttca tataaatatt ctcgacatga tattaataat atccaca
//
LOCUS       DAPHCV010000457          417 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.463, whole genome shotgun sequence.
ACCESSION   DAPHCV010000457 DAPHCV010000000
VERSION     DAPHCV010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.463"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..119)
                     /locus_tag="RH033_005117"
     CDS             complement(<1..119)
                     /locus_tag="RH033_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDV2994139.1"
                     /translation="MTNHIHFRCPCCHGSQYRTSSFDVSDMNPFGAKCIFCKS"
     gene            complement(130..342)
                     /gene="cspI"
                     /locus_tag="RH033_005118"
     CDS             complement(130..342)
                     /gene="cspI"
                     /locus_tag="RH033_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="HDV2994140.1"
                     /translation="MSNKMTGLVKWFNPEKGFGFITPKDGSKDVFVHFSAIQSNDFKT
                     LTENQEVEFGIENGPKGPAAVHVVAL"
BASE COUNT          142 a           84 c           73 g          118 t
ORIGIN      
        1 attgatttac aaaagataca ttttgccccg aaaggattca tgtcagaaac atcaaaagat
       61 gatgttctat actgggaacc atgacaacac gggcatctaa agtgaatatg gtttgtaata
      121 ttgtctacct caaagcgcca ctacatgaac agcggcagga cctttaggtc cgttctcaat
      181 accaaattca acttcctgat tctcagttaa tgttttgaaa tcgttgctct gaattgctga
      241 gaaatggaca aacacatctt tgctgccatc tttcggcgtg atgaaaccaa aacctttttc
      301 agggttaaac catttcacta aaccagtcat tttgttagac ataattatta ccttttgaag
      361 aaattagccc ttgggcagaa tggtccgaaa aaaaaatatc agagagaaaa accaaca
//
LOCUS       DAPHCV010000458          417 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.464, whole genome shotgun sequence.
ACCESSION   DAPHCV010000458 DAPHCV010000000
VERSION     DAPHCV010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.464"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
BASE COUNT          138 a           90 c           67 g          122 t
ORIGIN      
        1 tcaacatctt cagacttcca tacatactgg gtgttaattt ttcactttta cagccagcat
       61 ggagttttct gtcctaaaaa cagagcagat ttactctgga gttattttcc tgttcacgta
      121 ttcagtacag cagaaatccc gtgataacga gacagtaaac catcgtaatc agcgtctgta
      181 actccatcgc tttaccccag aaaagttaag ccatataatg tgaggaatat aagtcgtcgt
      241 atccggtaag tacagataac cacaacataa gctcattcag taaattttat ctctgaacaa
      301 acgactatgg catgctcatt tatactattc ataagaaagt gtgattatct gtaagcatta
      361 accatcaaat catataacca tactaaactg gcggatcatc agcaccatta gcaggta
//
LOCUS       DAPHCV010000459          416 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.465, whole genome shotgun sequence.
ACCESSION   DAPHCV010000459 DAPHCV010000000
VERSION     DAPHCV010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.465"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            43..>416
                     /locus_tag="RH033_005119"
     CDS             43..>416
                     /locus_tag="RH033_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HDV2994141.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILN"
BASE COUNT           92 a          111 c          111 g          102 t
ORIGIN      
        1 tggtgataaa cttatcatcc ccttttgctg atggagctgc acatgaaccc attcaaaggc
       61 cggcattttc agcgtgacat cattctgtgg gccgtacgct ggtactgcaa atacggcatc
      121 agttaccgtg agctgcagga gatgctggct gaacgcggag tgaatgtcga tcactccacg
      181 atttaccgct gggttcagcg ttatgcgcct gaaatggaaa aacggctgcg ctggtactgg
      241 cgtaaccctt ccgatctttg cccgtggcac atggatgaaa cctacgtgaa ggtcaatggc
      301 cgctgggcgt atctgtaccg ggccgtcgac agccggggcc gcactgtcga tttttatctc
      361 tcctcccgtc gtaacagcaa agctgcatac cggtttctgg gtaaaatcct caacaa
//
LOCUS       DAPHCV010000460          400 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.466, whole genome shotgun sequence.
ACCESSION   DAPHCV010000460 DAPHCV010000000
VERSION     DAPHCV010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.466"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>400
                     /locus_tag="RH033_005120"
     CDS             <1..>400
                     /locus_tag="RH033_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDV2994142.1"
                     /translation="QIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNYTYIPTITVLES
                     LQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIILLNSHDGTSSYQ
                     MLPGYFRFVCQNGCVCGQSLGEVRVPHRGNV"
BASE COUNT           92 a          118 c          113 g           77 t
ORIGIN      
        1 ccagatacgc cgtgaccgtc cgctgacaca cgaagaactg atgcactatg tgccggggat
       61 tttcggggaa gataaacaca cgtcccgaag ccagaactat acgtacatcc ccaccatcac
      121 cgtactggaa agcctgcagc gggaaggctt tcagccattc ttcgcctgcc agacccgtgt
      181 gcgcgacccg ggccgccggg gatacacaaa acacatgctg cgtctgcggc gggccggaga
      241 gataaacgga gaacatgtcc ctgaaattat tctgctcaac tctcatgacg gtacctccag
      301 ctaccagatg ctgccggggt acttcaggtt cgtctgccag aacggctgcg tctgtggtca
      361 gtctctgggg gaagtacgtg ttccgcaccg gggaaatgtg
//
LOCUS       DAPHCV010000461          379 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.467, whole genome shotgun sequence.
ACCESSION   DAPHCV010000461 DAPHCV010000000
VERSION     DAPHCV010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.467"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(1..319)
                     /locus_tag="RH033_005121"
                     /pseudo
     CDS             complement(1..319)
                     /locus_tag="RH033_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT          111 a           73 c           81 g          114 t
ORIGIN      
        1 gcacccaacc taactccgtt tcttcaaatt cacttgggaa aagcgagcgg atttccacca
       61 gaagcggttt aaaatctgca ctactacgta ctttttgacg caactcggca cgagattgca
      121 gagcctccgg gattggattt cctgcatcca gagcgttatc aatcactgga tcaaaatcca
      181 caaaccagga tttgaacagg gtttgggaca tttgttccaa ggattgattg atattaagag
      241 ataaatttat cttttgatca aagtggcgta aaaaatgtcc aatggatact cttttctcac
      301 tgagcttagg gatggtaacg gatattgtat taattattgt ttggttaatg agaggttggc
      361 ctgaaccagt actataatt
//
LOCUS       DAPHCV010000462          376 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.468, whole genome shotgun sequence.
ACCESSION   DAPHCV010000462 DAPHCV010000000
VERSION     DAPHCV010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.468"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..159
                     /locus_tag="RH033_005122"
     CDS             <1..159
                     /locus_tag="RH033_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDV2994143.1"
                     /translation="APQYNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARDG
                     GRLSGAYR"
     gene            159..>376
                     /locus_tag="RH033_005123"
     CDS             159..>376
                     /locus_tag="RH033_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV2994144.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKCEHPSLKAFLERHGGVRAFQ"
BASE COUNT           96 a           80 c          119 g           81 t
ORIGIN      
        1 gccccgcagt acaacatcga gatcaataac gatggcacga acgggcagat aggtccggct
       61 gccctgaagg tggtttatga ccttgggaaa aaagcggcag cggactttat gcaacagcag
      121 gcccgtgatg gtggtcggtt aagtggagca tatcggtaat ggagacgttt cactggaaag
      181 tgcgcccgga tatgaatgtg gtatcagagc cgaaagtggt gacagtgaag ctgggcgatg
      241 gttatgaaca gcgtcgtgcg gcgggactga ataaccagtt gtcgacttac agcgtgacga
      301 tacgtgttcg taaatgtgaa cacccatctt taaaagcctt tctggaacgg cacggtggcg
      361 tccgcgcatt tcagtg
//
LOCUS       DAPHCV010000463          375 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.denovo.469, whole genome shotgun sequence.
ACCESSION   DAPHCV010000463 DAPHCV010000000
VERSION     DAPHCV010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.denovo.469"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>375
                     /locus_tag="RH033_005124"
     CDS             <1..>375
                     /locus_tag="RH033_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TraC family protein"
                     /protein_id="HDV2994145.1"
                     /translation="VDAQAFIDIVGEMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFD
                     LNVRADYLTLGLRENGRNSTARILNFHLARNPEIAFLWNMADNYSNLLNPELSISCPF
                     ILTLTLVVEDQVKTHSEANLKY"
BASE COUNT           91 a          103 c           98 g           83 t
ORIGIN      
        1 cggtggatgc acaggccttt atcgatattg tcggggagat gattaaccat aacccggact
       61 ccctgtaccc gaaaagacgt cagctggacc cgtattctga tctgaattat cagtgtgtgg
      121 aggacagttt tgacctgaat gtccgggctg attacctgac gctgggcctg cgtgagaacg
      181 gcaggaacag cacggcccgt atcctgaatt tccatctggc ccgtaacccg gaaatcgcct
      241 tcctgtggaa tatggccgac aactacagca acctgctgaa cccggaactg tccatctcct
      301 gtccgttcat cctgacgctg actctggtgg tggaagacca ggtgaaaact cacagcgaag
      361 ccaacctgaa gtaca
//
LOCUS       DAPHCV010000464        13267 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.guided.120, whole genome shotgun sequence.
ACCESSION   DAPHCV010000464 DAPHCV010000000
VERSION     DAPHCV010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.guided.120"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1583
                     /gene="mscM"
                     /locus_tag="RH033_005125"
     CDS             <1..1583
                     /gene="mscM"
                     /locus_tag="RH033_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HDV2994146.1"
                     /translation="FIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDREF
                     SGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAI
                     LASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEM
                     LAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWS
                     EIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLEL
                     AILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGL
                     QEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKA
                     FITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLV
                     DLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGK
                     QTGRTLTSAGKGRQAGSL"
     gene            complement(1614..1928)
                     /locus_tag="RH033_005126"
     CDS             complement(1614..1928)
                     /locus_tag="RH033_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HDV2994147.1"
                     /translation="MSARMFILCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEDDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(1925..2239)
                     /locus_tag="RH033_005127"
     CDS             complement(1925..2239)
                     /locus_tag="RH033_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2994148.1"
                     /translation="MDDASRDPVITEDEIRELQFSAGDVAEIEQTVLSFVDTRHTRKV
                     AMVVGNTINTLKERDGPRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPI
                     IS"
     gene            complement(2302..3804)
                     /gene="yjeM"
                     /locus_tag="RH033_005128"
     CDS             complement(2302..3804)
                     /gene="yjeM"
                     /locus_tag="RH033_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HDV2994149.1"
                     /translation="MPHSIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(4023..5000)
                     /gene="epmA"
                     /locus_tag="RH033_005129"
     CDS             complement(4023..5000)
                     /gene="epmA"
                     /locus_tag="RH033_005129"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HDV2994150.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            5325..7133
                     /gene="frdA"
                     /locus_tag="RH033_005130"
     CDS             5325..7133
                     /gene="frdA"
                     /locus_tag="RH033_005130"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HDV2994151.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQAMERASTAGNGNDAAINAQVAGVEQRLKDLVNQDGGENWA
                     KIRDEMGMAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            7126..7860
                     /gene="frdB"
                     /locus_tag="RH033_005131"
     CDS             7126..7860
                     /gene="frdB"
                     /locus_tag="RH033_005131"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HDV2994152.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            7871..8266
                     /gene="frdC"
                     /locus_tag="RH033_005132"
     CDS             7871..8266
                     /gene="frdC"
                     /locus_tag="RH033_005132"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HDV2994153.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            8277..8636
                     /gene="frdD"
                     /locus_tag="RH033_005133"
     CDS             8277..8636
                     /gene="frdD"
                     /locus_tag="RH033_005133"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDV2994154.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            8699..9832
                     /locus_tag="RH033_005134"
     CDS             8699..9832
                     /locus_tag="RH033_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HDV2994155.1"
                     /translation="MFKMTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGI
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDDVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANASIGLFGALAVKPSGLSFEQAMQTRVFQPLKLTHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDITEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            9921..10454
                     /gene="blc"
                     /locus_tag="RH033_005135"
     CDS             9921..10454
                     /gene="blc"
                     /locus_tag="RH033_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HDV2994156.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(10451..10768)
                     /gene="sugE"
                     /locus_tag="RH033_005136"
     CDS             complement(10451..10768)
                     /gene="sugE"
                     /locus_tag="RH033_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDV2994157.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(10943..11089)
                     /gene="ecnB"
                     /locus_tag="RH033_005137"
     CDS             complement(10943..11089)
                     /gene="ecnB"
                     /locus_tag="RH033_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HDV2994158.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(11200..11325)
                     /gene="ecnA"
                     /locus_tag="RH033_005138"
     CDS             complement(11200..11325)
                     /gene="ecnA"
                     /locus_tag="RH033_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HDV2994159.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(11377..11943)
                     /gene="efp"
                     /locus_tag="RH033_005139"
     CDS             complement(11377..11943)
                     /gene="efp"
                     /locus_tag="RH033_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDV2994160.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            11985..13013
                     /gene="epmB"
                     /locus_tag="RH033_005140"
     CDS             11985..13013
                     /gene="epmB"
                     /locus_tag="RH033_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HDV2994161.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIERMEKGNPDDPLLRQVLTSQEEYIVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFAHSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTQLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFVVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         3388 a         3439 c         3357 g         3083 t
ORIGIN      
        1 tgtttatcgc tcattttggc tggccgcgcg aacgggtttc ccgtgggatg cgctactacc
       61 tgatgagcat cgggcttatt gtgccgctga ttatggcgct gatgatgttc gataacctcg
      121 acgaccgtga attctccggt tcgctgggac ggctttgctt tatcctcatt tgcggtgcgc
      181 tggcggtggt cactctcagt ctgaaaaagg ccgggatccc gctgtatctc aacaaagaag
      241 gcagcggcga caacattacc aaccatatgc tgtggaacat gatgattggc gcaccgctgg
      301 ttgccattct ggcgtcggcg gtgggttatc tggcaacggc gcaggcgctg ttagcgaggc
      361 ttgaaacctc ggttgccatc tggttcttgc tactggtggt ttatcacgtt atccgccgct
      421 ggatgctgat ccagcgccgc aggctggcgt ttgaccgggc gaagcatcgc cgggcagaga
      481 tgttagcgca gcgtgcgcgt ggcgaagagg aagcgcatca tcacagtagc ccggaaggag
      541 caattgaagt cgatgaaagc gaagtcgatc tcgatgccat cagtgcgcaa tccttgcggc
      601 tggtgcgctc aattttgatg ttgatcgccc tgctttcggt cattgtgctg tggtcagaaa
      661 tccattccgc tttcggcttc ctcgaaaata tttcgctgtg ggatgtcact tccacggtac
      721 agggcgtaga aagcctggag ccgattaccc tcggtgcggt gctgattgcc attctggtgt
      781 ttatcatcac cacgcagctg gtgcgcaacc tgcccgcgct gctggaactg gcgattctgc
      841 agcacctgga tttaacgccg ggtacgggct acgccatcac caccatcacc aaatatctgc
      901 tgatgctgat tggcgggctg gtcggcttct cgatgattgg tattgagtgg tcgaaattgc
      961 agtggctggt cgccgcgctc ggtgttggtc tcggttttgg tttgcaggaa attttcgcca
     1021 actttatctc tggcctgatt atcctgttcg aaaaaccgat tcgcattggc gatacggtaa
     1081 cgatccgcga tcttaccggt agtgtgacga aaattaacac ccgcgccacc accatcagcg
     1141 actgggaccg caaagaaatt atcgtgccga acaaggcgtt tattaccgag cagtttatca
     1201 actggtcgct ctctgactcg gtcacgcgcg tggtgttgac gattccggcc cctgccgatg
     1261 ccaacagtga agaagtgacg gaaatcctgc tcaccgcagc gcgtcgctgc tcgctggtga
     1321 tcgacaaccc ggcgccggaa gtcttcctgg tggatctgca acaggggatt caaattttcg
     1381 agctgcgtat ttacgccgct gagatgggtc accgtatgcc actacgccat gagattcacc
     1441 agctaattct ggcaggattc catgcccacg gtatcgatat gccattcccg cccttccaga
     1501 tgcgtctgga aagcctgaat ggtaaacaaa cggggagaac attaacttct gcgggcaaag
     1561 gtcgtcaggc gggaagtttg taaggtcttt gtggccggat gaacccatcc ggcttataag
     1621 caaaactgat agcgcagaaa aaactgccac agccaatagc cgaaaatagc cagcgcggtg
     1681 gcgtacataa accagccgcg tttttttcgc cacaaagcga accagagaaa agggaaaaag
     1741 agcggtaacg tcattatcac accgacatca cgggtatcgt catcttcgag atacatcagc
     1801 acatcgcaga cgttattaat cccgcgtcca tcaatcatcc actctttgtc cagcgcggaa
     1861 gtgatggtta tccagaggaa cgccacaata aaccagatgc agcacaagat aaacatgcgc
     1921 gcactcatga gataatcggt cgcttaattt cgctatagcg catacgaaac aaatcgccgt
     1981 atcccactaa ttctccgcga aaaaccagac atctaataag ataagcgcaa tagatatccg
     2041 gcaggtttcc ccaccgcgga ccatcgcgct ctttgagggt attaatagtg ttacccacca
     2101 ccatcgctac tttgcgggtg tggcgggtat cgacaaaaga gaggaccgtt tgctctattt
     2161 ccgccacatc cccggcagaa aattgcagct ccctgatttc atcttcggtt atcactggat
     2221 cccgactagc gtcgtccata acgtcttcct ttccagacaa ctcatcgccg gagagacgat
     2281 gagttgcagg atttcatatg atcagtcgag agctaactca ggtttgttcg ccatgcgact
     2341 gcaatagttc tggtaaatcg ccatcgctaa cagcgagaag aagacagggc cgccaatcat
     2401 ccacaatgtg ctgtcccagt ctccagcttc aaccacaggt tgaatgatgg tgaagacgtt
     2461 cgcaaatgtc accaccagta cgacaaccac tgttgcaatc attgccgaca tatgcgtttt
     2521 gaaaatcaca aacggtctgt cgagatcctg acgtgcttta aagaatggga aagccagcgc
     2581 gaggaacagg taaggaagcg tcatagacac gttcgccatc agcgtcagct tgttaaagaa
     2641 cgccgatgcg gtaccgccac caaacgaaac cagcaggatg aagacagtaa ccaacccgca
     2701 ctgcatccac atggcgatag acggcatccc catcgcattc aggcgcgtca tcggttccgg
     2761 ccacaacgct ttcggcgtcc cctggatgat ggctttcagc ggtgaatagc aaagcgtaaa
     2821 gaacgcaccg gtataagcga ggaacatcga aagtccggta atacgcgcaa accatacgcc
     2881 cagcgacaat gacgcttcag gtgacaaatg cagtgcgtta cccagcgtca ccccgaggct
     2941 cttcatcagc acataggtaa tattgccgag gttaacggaa ccattactta atacctgctg
     3001 ccagtttgtg ctgacgcccc ataaaaatat tgccagcgaa taaccgattg aaataacaat
     3061 agcggcaaaa acaatacctt tggcaaagtt cttttctgga ttttccgttt tatcgaccag
     3121 accaccaacc gcttcaattc caccataggc aaaaatagca aataccacaa acgatagcat
     3181 tgccagaccg gactgataac ctgggttcgg tgatgcaagg aaattaatat cctgcgcgaa
     3241 atgcccacca tttaataaca aaatagtaat gcttactaac agcaatacta aattcagaca
     3301 cattactgca ataccgccca ccgcagtaat gcgggcaatt ttattaatcc cttttgaagc
     3361 aacgacggtg accagaatca tccatgccac tgccagcaga ccaaccacct gcgtaggctc
     3421 cagtccggca atacgccagt gctgggtcat gtcgctacca tagaggaatg ttgagaacgg
     3481 tacccaaact ttcgcggagg tactcaccat ccagatgata taagaggaaa accacataaa
     3541 cgtaccaatg aaggcaaaac gtggtccgac actattattc atccaggaat agataccgcc
     3601 ttcttctttg cgataagcag ctcccatttc agccatcatt aaggcgaatg gaataaagaa
     3661 taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt aataagccga
     3721 tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc ctatcagact
     3781 catctttttt atcgagtgag gcatgaaacc atccttcatt tcagcgcgtg tataaaacgc
     3841 gcccactgaa ctgcactgcc caacattgag cgggcagcgt aaaaaccaat acagaattga
     3901 tacctggatg atatcaaata tccagtctta aattgtggcc tttagcggaa aaatgaaaac
     3961 atatgctaca gaatggcgct tatcacgcca ttcttcgcgg ttaattcagt aatttttcag
     4021 aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc gcccagcgcc
     4081 aacatcacca gacgatcaac gcctaatgcc acgccggaac agtcaggcat accgactttc
     4141 aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc cgcgcgctta
     4201 cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgtcaattc atggaaacca
     4261 ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg atgatcttcg
     4321 gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa ggtcggtttt
     4381 tctttgccaa tatttggctc tacgccaaag gtaaacagca attgtagaag cgtgtcgcgg
     4441 tcttcttcag tatcggcaac gttgctcaaa tccagtttcg ctgccacttc ccgcagttgc
     4501 gttttgtcgg cagagagtgg gtcaatttcc agataacgca agaaagcttg ttgataagaa
     4561 aggctttctg ctgccggaca gtccagcacc tgttgcaaga gatcgtccac ctcgttcatc
     4621 aaccggtaca tatcatagtg cggtcgatac cactcaagca tagtgaactc agggttgtga
     4681 taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg cccacaacca
     4741 gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag attcatcccc
     4801 tgcgaatgcc cggggcctac gaaacgtgtc tcaaacggga ccaaatgaat atcggttacc
     4861 gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc aaagaaacga
     4921 cggatctccg ccataatcgc cgcgcgtttt aataagttag gaatggatgc gctcggctgc
     4981 caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac gaagtctact
     5041 cgcaacgcga cggcgagaca aattttacgc aggaatcaaa caagggtggg cagtgactaa
     5101 aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat aatttagccc
     5161 tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca gactatactg
     5221 ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca ggcacttacc
     5281 ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa acctttcaag
     5341 ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt gctgccgcgc
     5401 aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg cgtagccata
     5461 ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc ttcgaatatc
     5521 actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc gtggattatt
     5581 tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtgggggtgc ccatggagcc
     5641 gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc gagcgcacct
     5701 ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag acctctctgc
     5761 aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg gttgatgatg
     5821 gtcatgttcg cggcctggta gcaatgaaca tgatggaagg cacgctggtg cagatccgtg
     5881 ctaacgcggt cgttatggct accggcggtg cgggtcgcgt ttatcgttac aacaccaacg
     5941 gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt ccgctgcgtg
     6001 acatggaatt cgttcagtat cacccaaccg gtctgcccgg ttccggtatc ctgatgacag
     6061 aaggctgccg cggtgaaggt ggtattctgg tcaacaaaaa tggctaccgt tatctgcaag
     6121 attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat atggaactgg
     6181 gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc aacaccatct
     6241 ccacgccacg tggcgatgtg gtttatctcg acctgcgtca cctcggcgag aaaaaactgc
     6301 atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc gatccggtta
     6361 aagaaccgat tccggtacgt ccgaccgcac actacaccat gggcggtatc gaaaccgatc
     6421 agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc tctgttggtc
     6481 tgcacggtgc gaaccgtctg ggctccaact cgctggcgga actggtggtc ttcggtcgtc
     6541 tggccggtga gcaagctatg gaacgcgcat ctactgccgg taatggcaac gatgcagcaa
     6601 tcaatgctca ggttgctggc gttgaacagc gcctgaaaga tctggttaac caggatggcg
     6661 gcgaaaactg ggctaagatc cgcgacgaaa tgggcatggc catggaagaa ggctgcggta
     6721 tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag ctgcaggaac
     6781 gcttcaagcg cgtgcgcatc accgacactt ccagcgtgtt caacaccgac ctgctctaca
     6841 ccattgagct gggccacggt ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac
     6901 gtaaagagtc ccgcggcgcg caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg
     6961 tcaacttcct caaacacacc ctcgccttcc gcgatgctga tggcacaact cgcctggagt
     7021 acagcgacgt gaagattact acgctgccgc cagctaaacg tgtttacggt ggcgaagcgg
     7081 atgcagccga taaggcggaa gcagccaata agaaggagaa ggcgaatggc tgagatgaaa
     7141 aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc gcatagcgca
     7201 ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg ctacatcaaa
     7261 gacaacctgg caccggacct gagctaccgc tggtcctgcc gtatggcgat ttgtggctcc
     7321 tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac
     7381 accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga tctggtggtc
     7441 gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat cggcaactcc
     7501 cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcgaa gtatcaccag
     7561 ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt tggcctgaac
     7621 ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga agatagccgc
     7681 gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggcgt atggagctgt
     7741 actttcgtgg gctactgctc cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt
     7801 cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa accacgctaa
     7861 ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg
     7921 gaaaaaattg ccgttttatc gcttctacat gctgcgcgaa ggcacggcgg ttccggctgt
     7981 gtggttcagc attgaactga ttttcgggct gtttgccctg aaaaatggcc cggaagcctg
     8041 ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc tgatcactct
     8101 ggcggcagcc ctgctgcaca ccaaaacctg gtttgagctg gcaccaaaag cggccaatat
     8161 cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagcctct gggcggtaac
     8221 tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag cctgagatga
     8281 ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc ggggccggtg
     8341 gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggttggtatt ctgctgccac
     8401 tggggctgtt tccgggtgac gcactgagct acgagcgcgt tctggcgttc gcgcagagct
     8461 tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc
     8521 gtatgcacca cgcgatgcac gatctgaaaa tccacgtacc tgcgggcaaa tgggttttct
     8581 acggtctggc tgctatcctg acagttgtca cgctgattgg tgtcgttaca atctaacgca
     8641 tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac cagaccctat
     8701 gttcaaaatg acgctctgcg ccttattaat taccgcctct tgctccacat ttgctgcccc
     8761 tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc aacaaaagat
     8821 ccccggtata gcggtggcgg taatttatca gggtaaacct tattacttta cctggggcta
     8881 tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt taggttcggt
     8941 cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt
     9001 aagcgatccc gcgacaaaat actggcctga acttaccgct aaacaatgga atgggatcac
     9061 actattacat ctcgcgacct acaccgctgg cggcctgcca ttgcaggtgc cggatgacgt
     9121 taaatcctca agcgacttgc tgcgcttcta tcaaaactgg caacctgcat gggctccagg
     9181 aacacaacgt ctgtatgcca acgccagtat tgggttgttc ggtgcactgg cggtgaaacc
     9241 atccggtttg agctttgaac aggcgatgca aactcgtgtt ttccagccac tcaaactcac
     9301 tcatacgtgg attaatgtgc cgtccgcaga agaaaagaat tacgcctggg gatatcgcga
     9361 aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaggcttatg gtgtgaagtc
     9421 gaccattgaa gatatggccc gctgggtgca aagcaattta aaaccccttg atatcactga
     9481 gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa ccggcgatat
     9541 gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca tcatcattaa
     9601 cggcagtgac aataaaattg cactggcagc acgccccgta aaagcgatta cgcccccaac
     9661 tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat ttggtagcta
     9721 tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca aaaactatcc
     9781 caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt aaaatttcat
     9841 cgggtccgaa tttttggacc ttttctccgc ttttccttgc tgtcatctac acttagaaaa
     9901 aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg acagctgcat
     9961 ttctggtcgt tgcctgcagt tcccctacgc cgccgcgtgg cgtgaccgta gtgaataatt
    10021 tcgacgctaa acgttatctc ggtacctggt atgagattgc ccgttttgat caccgctttg
    10081 aacgtggact ggaaaaagtc accgcaacat acagcctgcg tgatgacggc ggcctgaatg
    10141 tcattaataa aggctataac cctgacagag aaatgtggca gcagagtgaa gggaaagcgt
    10201 actttaccgg agcaccaact cgcgctgcgc tgaaagtgtc attctttggt cctttctatg
    10261 gcggttataa cgttattgca ctcgatcggg aataccgcca tgcgctggtt tgcggcccgg
    10321 accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa gtgaaacagg
    10381 agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt tgggtacagc
    10441 agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg attaacgcca
    10501 gactcgccag gcgcatcgga ttagcggact caccgagcag cacaataccg gtgatggccg
    10561 cgccgactgc gccaataccc gtccacacgg cataagccgt ccctactggt aacgatttca
    10621 tcgcccaggc aagtagcgcc atactgacaa tcatcgccgt cacagtaata acacttggcg
    10681 tcaaacgact aaaaccgtgg gtatatttca ggccaacggc ccataccact tccagcaggc
    10741 cagcaataac taagataatc caggacatat caggctccgg agaattgggg ccgtccccgg
    10801 tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg ttgaacgggt attttgccag
    10861 gtggcagggg aatttcaact attaccttag tgactatcgc aaaaaaacaa acggcacgac
    10921 acagctgtcg taccgttatt gcttattgct gtgcttttgt tgctgcacca gaaatcgcgt
    10981 taccgccatc agagatatct tcaccaacgc cacgcgtggt gttgcaggca gttaatactg
    11041 ttgaaagcac cagaacagaa aagatcgctg caattgtctt cttcaccata acgtcttcct
    11101 tttaggtaat gttatttatg tttgcctata gcaaattaat aatagacaac atcgccaaaa
    11161 atgacggaaa tctgatgatt tttcggaaga gaagaaaaat tagctggcag cgcgggaaat
    11221 ggagttgccg agatgtttga tgtcttcgcc gaaaccgcga gcggtgttac agcccgtgag
    11281 cagcgtgctg gcaagcaaaa caagaacgat aaggcgtttc atcatctctg accagaataa
    11341 gtgatggtgc agcctgcagg ccgcaccaca accgtattac ttcacgcgag agacatattc
    11401 accagagcgg gtatccactt tgatgacttc gccgatctgc acgaacagcg gaactttaac
    11461 cacagcgcca gtagacaggg tagccggttt gccgccagta cctgcggtat cacctttcag
    11521 gcccggatcg gtatcaacga tttccagttc aacgaagttc ggcggagtaa cggagatcgg
    11581 ctgaccattc cacagagtta cgatacactc tgcctgatcc agcaaccatt tagcgttgtc
    11641 gccaattgct tttgcatcag cagacagttg ctcgaaagtt tcgttgttca tgaagtgcca
    11701 gaactcaccg tcgttgtaca ggtaagtcag gttcatatcg acaacatcag cgccttcagc
    11761 ggaatcagta gatttgaagg ttttttctac gcgagtaccg gtcagcagac gacgcagttt
    11821 aacgcgagca aatgcctggc ctttacccgg ttttacgaat tcactcgctt caaccgcgta
    11881 aggttcgccg tctaacatga ttttaagacc agcacgaaaa tcgttgctat agtacgttgc
    11941 cataaggccc tctgaaattt gttaattggt agctaagcca caaaatggcg catattgtaa
    12001 ccctaaatac cccatccaga gaagattggt taacgcaact tgccgatgtt gtgaccgatc
    12061 ctgatgaact tctgcgtctt ttgaatatag acgcggacga aaaactgttg gccggacgca
    12121 gtgctaaaaa gctgtttgcc ctgcgtgtgc ctcgatcatt tattgaacgc atggagaaag
    12181 gtaatcctga tgaccctctt ttgcgtcagg tgcttacatc gcaagaggag tatattgtag
    12241 cgccgggatt ctccaccgat ccgctggaag aacagcacag cgtggtgcct ggtttgttgc
    12301 ataaatacca caaccgagcg ctattgctgg taaaaggcgg ctgtgcggta aattgccgct
    12361 attgcttccg ccgccacttc ccgtatgccg aaaatcaggg caataaacgt aactggcaga
    12421 cggcacttga gtatgttgct gcgcatccgg aactggacga gatgattttc tccggcggcg
    12481 atccgctgat ggcgaaagat cacgagctgg actggttgct cacacaactg gaagccatcc
    12541 cgcatatcaa acgtctgcgg attcacagcc gtctgccgat tgtgattccg gcacgcatca
    12601 cagatgcgct ggttgaacgt tttgctcatt ctacgctgca aatcttgctg gtgaatcaca
    12661 tcaaccatgc caatgaggta gatgaaacat tccgccaggc gatggctaaa ttgcgccgtg
    12721 tcggcgtcac ccagcttaac cagagcgttc tgttacgtgg cgtgaacgat aacgcgcaaa
    12781 cgctggcaaa tttgagtaat gcactgtttg acgccggtgt gatgccctat tacctgcacg
    12841 tgctcgataa agtgcagggc gcggcgcatt tcgtggtgag cgatgatgaa gcacggcaga
    12901 ttatgcgtga gttgttgacg ttagtatcgg gatatctggt gccaaaactg gcgcgcgaaa
    12961 ttggtggcga acccagcaag actccgctgg atctccagct acgccagcag taactgatta
    13021 aacagggaag ttaaacatta aaaatgcctg atgcgctacg cttatccggc ctacatcgtg
    13081 ctttgaaatt gaatgaattt tctatgaact gtaggccaga taaggcgttt acgccgcatc
    13141 cggcaagagc aacgaacacg agtacagcaa cctgcattaa actggaaagt ttaatctttg
    13201 cccgttgcat tagccttaaa tattcgtttg atatgttttt tattacttac ataattaaca
    13261 tcaataa
//
LOCUS       DAPHCV010000465         8756 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.guided.158, whole genome shotgun sequence.
ACCESSION   DAPHCV010000465 DAPHCV010000000
VERSION     DAPHCV010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.guided.158"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            78..1133
                     /gene="dinB"
                     /locus_tag="RH033_005141"
     CDS             78..1133
                     /gene="dinB"
                     /locus_tag="RH033_005141"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDV2994162.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            1130..1582
                     /locus_tag="RH033_005142"
     CDS             1130..1582
                     /locus_tag="RH033_005142"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV2994163.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFLRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFITRIGHYIDMLFVEPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGTWFTNFYMRYKPQH"
     gene            1772..2911
                     /locus_tag="RH033_005143"
     CDS             1772..2911
                     /locus_tag="RH033_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HDV2994164.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRNSLGSIGGGNHFVELQQIDQIIDAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALRYIAEHDDALAFARINRQL
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWLVKPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVLAGLIIPVARLRPVLTLKNSGGKKG"
     gene            2908..3522
                     /locus_tag="RH033_005144"
     CDS             2908..3522
                     /locus_tag="RH033_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HDV2994165.1"
                     /translation="MILLQLSSAQGPEECCLAVRKALDRLIKEATRQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAALKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(3579..5036)
                     /gene="pepD"
                     /locus_tag="RH033_005145"
     CDS             complement(3579..5036)
                     /gene="pepD"
                     /locus_tag="RH033_005145"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HDV2994166.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVDTYQEILKNELAEKEKNLALLLDSVANDKAALTATSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            5297..5755
                     /gene="gpt"
                     /locus_tag="RH033_005146"
     CDS             5297..5755
                     /gene="gpt"
                     /locus_tag="RH033_005146"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDV2994167.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            5847..7091
                     /gene="frsA"
                     /locus_tag="RH033_005147"
     CDS             5847..7091
                     /gene="frsA"
                     /locus_tag="RH033_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HDV2994168.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKVVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            7149..7550
                     /gene="crl"
                     /locus_tag="RH033_005148"
     CDS             7149..7550
                     /gene="crl"
                     /locus_tag="RH033_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HDV2994169.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVRIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(7589..8644)
                     /gene="phoE"
                     /locus_tag="RH033_005149"
     CDS             complement(7589..8644)
                     /gene="phoE"
                     /locus_tag="RH033_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HDV2994170.1"
                     /translation="MKKSTLALVVMGIVASVSVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPISGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYVDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
BASE COUNT         2140 a         2139 c         2250 g         2227 t
ORIGIN      
        1 gcgttaaatg ctgaatcttt acgcatttct caacccctga aattactgta tactttacca
       61 gtgttgagag gtgagcaatg cgtaaaatca ttcatgtgga tatggactgc tttttcgcag
      121 cggtggagat gcgcgataat cccgccctgc gcgatatccc tattgctatt ggcggcagcc
      181 gcgaacgacg gggggtgatc agcaccgcca attatcccgc gcgtaaattt ggcgtacgta
      241 gcgctatgcc gacagggatg gcgctcaaat tatgcccgca tctcaccttg cttccagggc
      301 gctttgacgc ctacaaagaa gcctcaaatc atatccgcga aattttctcg cgctatacct
      361 cgcgcattga accgttatca ctggatgaag cctatctcga tgtcaccgat agcgtccatt
      421 gccacggttc tgcgaccctc atcgcccagg aaatccgcca gacgatcttc aacgagctgc
      481 aactgacggc gtctgcgggc gttgcgccgg tcaagtttct cgccaaaatt gcttccgaca
      541 tgaataaacc caacggtcag tttgtgatta cgccggcaga agttccggca tttctgcaaa
      601 ccttaccgct ggcaaaaatt cccggcgtcg gcaaagtctc ggcggcaaaa ctggaagcga
      661 tggggctgcg gacctgcggt gatgtgcaaa agtgtgatct ggtgattctg cttaaacgct
      721 ttggcaaatt tggccgcatt ttgtgggagc gtagtcaggg gattgacgag cgcgacgtta
      781 acagtgaacg gttgcgaaaa tccgtcggcg tggaacgcac gatggcggaa gatattcatc
      841 actggtctga atgtgaagcg attattgagc ggctgtatcc tgaacttgaa cgccgtctgg
      901 caaaggtaaa acctgattta ctgattgctc gccagggggt gaaattaaag ttcgacgatt
      961 ttcagcaaac tactcaggag cacgtctggc cgcggctgaa taaagctgat ctaatcgcca
     1021 ccgcgcgtaa aacctgggat gaacgtcgcg gcgggcgcgg tgtgcgactg gtggggctgc
     1081 atgtgacgtt gcttgaccct caaatggaaa gacaactggt gctgggatta tgaataacat
     1141 acaaataaga aactatcagc ctggtgattt tcagcaacta tgcgctattt tccttcgagc
     1201 tgttacgatg acggcgagtc agcactattc tccacagcaa attgccgcct gggcgcagat
     1261 tgacgaatct cgctggaagg agaaactcgc gaaatcacaa gtgcgggttg cagttattaa
     1321 tgcacaaccg gttggtttta ttacccgcat tggacattat atcgatatgt tatttgttga
     1381 accggaatac acccgacgcg gagttgccag cgctttgtta aaaccattga ttaagtctga
     1441 atccgaactt acggtggacg ccagcataac cgcaaaaccc ttttttgaac gttatggttt
     1501 tcagacagtt aagcagcagc gcgttgaatg ccggggaacg tggtttacta atttttatat
     1561 gcgatataaa ccgcaacatt aaatccagct tgcaatgaaa ataacgcccg tctagtatgt
     1621 gcgcgttttc ttattccaca aactgcaagg aggtaaatca tgacaaatcc tttatcaatg
     1681 actctttgca gacctttcca ggattaattc ttttttcttg ccctggattc gtctgccatt
     1741 tcctgatttt tattcataaa tggaatggta catgggcaaa tatattcgtc ccttatccga
     1801 tgcggtattt accatcgcat ctgatgacct gtggatcgag agtttagcga tccaacaatt
     1861 acacaccacg gcaaatttac ccaacatgca gcgcgttgtg gggatgccag atttacatcc
     1921 cggacgcggc tacccgattg gcgcagcgtt cttctctgtt ggtcgttttt acccggcact
     1981 ggtcggcaat gatatcggct gcggcatggc gctatggcaa acagatattc tcgctcgcaa
     2041 atacaacgcc gataagtttg aaaagcgatt atcagatctg gatgacgttg ctgaagaaag
     2101 ctggctggag gaaaacctgc cgtcagcatt tgcacagcat ccgtggcgca actcgcttgg
     2161 ctccatcggt ggcggtaacc acttcgtaga actgcaacag attgatcaaa ttatcgacgc
     2221 ggaactgttt gcactggctg gtctggatgc gcagcatctg caactgctgg ttcatagcgg
     2281 ctcgagggga ttggggcagt ctattttaca gcggcatatt gcctcgtttt cgcatcatgg
     2341 tttgcctgaa ggcagtgacg acgcgctacg gtatattgcg gaacatgacg atgcgctggc
     2401 gtttgcgcgt attaatcgcc agctgatcgc tttgcgcata atgcaacaag ttaaggccac
     2461 cggaagtccg gttctggatg tggcgcataa ctttgttagc gcgtgccaaa tcggcgatca
     2521 acagggctgg ttgcatcgta aaggcgccac gccggatgac aacggtctgg tgattattcc
     2581 cggttcacgc ggcgattact cctggctggt taaacctgtt gcgaacgaaa aaacgctcca
     2641 ctcgctggcg catggggcgg ggcgtaaatg ggggcgcacc gagtgtaaag ggcgtctggc
     2701 agcgaaatac acggcgacgc aactctcgcg gactgaactt ggcagtcggg taatttgtcg
     2761 cgataaacaa ctcatctttg aagaagcgcc acaagcctat aaatcggctg aaagcgtggt
     2821 gcaatgtctg gtgctggctg ggttaattat tcctgtcgcg cgactgcgcc cggtgctgac
     2881 gctcaaaaac agtggaggga aaaaaggatg atcttgctac aactctcctc tgctcagggg
     2941 ccggaagaat gttgtctcgc agtgagaaaa gcactggaca ggctgattaa agaagctacc
     3001 cgacaggacg tcgcggtaac ggtgctggaa acagaaacgg gtcgctactc tgacacactg
     3061 cgttcggcgc tgatttctct ggatggcgat aacgcatggg ctctaagcga aagctggtgc
     3121 ggcactattc agtggatttg tccgagtccg tatcggcctc atcatgggcg caaaaactgg
     3181 tttctgggca ttgggcgttt taccgctgat gagcaggaac aatcggatgc aatccgttat
     3241 gagacgctgc gttcgtcggg gccgggcggt caacatgtca ataaaaccga ctcggcggta
     3301 cgcgccacgc atctggcatc cggtattagc gtgaaggttc agtcagagcg cagtcagcat
     3361 gctaacaaac ggctggcgcg attactgatt gcctggaagc tggaacaaca gcaacaggaa
     3421 aatagcgcgg cgctgaaatc gcagcggcga atgttccatc accagattga acgtggcaac
     3481 ccgcgacgaa cgtttacagg gatggccttt atcgaagggt aatgaaggat gaaactgccg
     3541 gaaggcgatt aaacgccatc cggcagcaaa tcaaataatt acttcgccgg aatttctttc
     3601 agcagttcag tcagcagtgt ccagtaatgg ccaacgcttt cgatatgaac ttgctcatcc
     3661 ggagagtgtg gaccggtgat ggttggcccg atagacacca tgtccatttc cggatacggt
     3721 tttttgaaca gaccacattc caggcccgcg tggataatct ggatgttcgg cgtcttgttg
     3781 aacagacgct gataggtttc acgtaccaga tgcattaccg gagagttagc gtccggctgc
     3841 cagccaggat atgcgccttt tgcttctgtt ttcgcacctg ccagtttacc cagtgaatcc
     3901 agcatgctca ctacgtagtc tttaccgctg tcgatcagag aacggatcag gcagtgaatt
     3961 tctacgttat tgtcggtcat ggtcaccaca ccgacgttca gggaggtttc gaccacacct
     4021 tttgccacgt cggagttgcg gatcacaccg ttcggggttg cgttcagcag acgaataaag
     4081 gtatcgcgag atgtcgccgt taatgcagct ttgtcgttcg ctacggagtc cagcagcaat
     4141 gccagattct tctctttttc tgccagttcg tttttcagga tctcctgata ggtatccacc
     4201 agagatttca gggcgtcgac tttatcagct gcgacagcaa tggtcgcaaa ggcttcacgc
     4261 gggatggcgt tacgcagtgt gccgccgttg aaatcaataa ggcgcaggtc cagttcttct
     4321 gcatgacccg ccaggaagcg caccagcagt ttgttggcat tacccaggcc aacgtggatt
     4381 tcaccgccgg agtgaccgcc tttcagacct tttaaggtta acttgaaggt ttcaaaacca
     4441 gctggaaccg cttcacgatc taaatgcagg ttggaggtga agtcgatacc tcccgcacaa
     4501 cccatgtaga tttcaccttc ttcttcggag tcggtgttaa tcagaatatc agcctgcaac
     4561 cagttgctct gtaagccaaa agcaccgtcc ataccggctt cttcggtcat ggtcagcagc
     4621 acttccagcg ggccgtgaac cacgttttcg tcagccagaa ccgccagtgc agaggccata
     4681 ccaatgccgt tatccgcacc cagcgtggtg ccgcgtgctt taacccattc gccatcaata
     4741 taaggctgga taggatcttt agtgaagtca tgcacggtgt cgttgttttt ttgcggcacc
     4801 atatcgaggt gggcctgcaa gacgaccggt ttacgatttt ccatacctgc ggtagcaggt
     4861 ttacgaatca ggatattacc tacctgatcg cgttcgacat ggaaaccttt ctcttttgcc
     4921 caaccaacaa tgtattcagc gagttgctct tcatgatagg acgggtgagg aatagaacag
     4981 attttggcaa aaatatccca cagcggctgt ggagataatt gagacagttc agacacgtta
     5041 agtctccttg tcgatcaccc gcaaaacagt attgcaggtc acagggttag cagaaaatgt
     5101 tgtcaacaca agacaggctt gcgagatatg tttgagaata ccactttatc ccgcgtcagg
     5161 gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgccaga
     5221 tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca
     5281 ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca
     5341 tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt
     5401 aagccgtggc ggtctggtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt
     5461 cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa
     5521 acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtag ataccggtgg
     5581 tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa
     5641 accggctggt cgtccgctgg ttgatgatta tgttgttgat atcccgcaaa atacctggat
     5701 cgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt
     5761 tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcgggtta caatagtttc
     5821 cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca
     5881 aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttagt cacggcgcac
     5941 aaccgcctgt gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta
     6001 accgtctgat gtggatctgg cgcggcattg acccccgtga aatcctcgac gtccaggcgc
     6061 gtattgtgat gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct
     6121 accgcggcgg caactggatt tatgagtggg ctacccaggc gatggtgtgg caacaaaaag
     6181 cctgtgcgga agaagatccg caactcagcg gtcgacactg gctgcatgcg gctacgttgt
     6241 acaacattgc tgcctatcct catctgaaag gagatgacct ggccgagcaa gcgcaggctt
     6301 tgtcaaaccg cgcctatgaa gaggccgctc agcgtctgcc gggcacgatg cggcagatgg
     6361 agtttaccgt acccggcggt gcgcccatca ccggcttttt gcatatgccg aaaggcgatg
     6421 gcccgttccc gacagtatta atgtgtggtg gtctggatgc gatgcagacg gactattaca
     6481 gcctgtatga acgttatttt gcgccgcgcg gcattgctat gctgactatt gatatgccgt
     6541 cggtgggctt ttcttcaaaa tggaagctca cccaggactc cagcctgttg catcagcacg
     6601 ttttaaaggc gctgcctaac gtaccgtggg tggatcacac tcgcgtcgcg gcctttggtt
     6661 tccgtttcgg cgctaacgtt gccgtgcgtc tggcatacct tgaatcgccg cgtctgaaag
     6721 tggttgcctg tcttggtccg gtagttcata ccctgttgag tgattttaag tgccagcaac
     6781 aggtgccgga aatgtatctt gacgttctgg ccagtcgttt agggatgcat gatgcttccg
     6841 atgaagcgtt gcgagtggag ttgaatcgct attcattaaa agtgcaagga ttgctgggac
     6901 gtcgctgccc aacgccaatg ttatcaggct actggaagaa cgatccgttc agcccggaag
     6961 aggactcacg cttaatcacg tcgtcatctg ctgacggtaa attattagag atcccattta
     7021 acccggtgta tcggaatttt gacaaaggcc ttcaggaaat cacagactgg atcgaaaaac
     7081 ggttgtgtta aaaatttgct aaattttacc aatttggtaa aacagttgca tcacaacagg
     7141 agattgcaat gacgttaccg agtggacacc cgaagagcag attgatcaaa aaatttaccg
     7201 cactaggccc gtatattcgt gaaggtaagt gcgaagataa tcgattcttt ttcgattgtc
     7261 tggctgtatg cgtcaacgtg aaaccggcac cggaagtgcg tgagttctgg ggctggtgga
     7321 tggaactgga agcgcaggaa tcccgtttta cctacagtta ccagtttggt ctgttcgata
     7381 aagcaggcga ctggacgagt gttcggataa aagatgccga agtggttgaa cgattggagc
     7441 acaccctgcg tgaatttcac gagaagctgc gtgagctgct ggcgacgctg aatctgaagc
     7501 tggaaccggc agatgatttt cgtgatgaac cggtgaagtt aacggcgtga atgaaatgtg
     7561 ccggatgcat cacatccggc aatatccatt aaaactgata ggtcatgcca accgcgacaa
     7621 tatcatcatt attaatattc aatttgttat cgctatccag ttggttgatt ttataatcaa
     7681 caaacgctga catatttttg ttgaaataat acgtagcacc aacatcgaca taattgacca
     7741 gatcttcatc accgatgccc tcaatatctt tccctttcga taagacataa cccagcgatg
     7801 gacgcagacc aaagtcaaac tggtattgag cgaccgcttc aaagttctgc gtcttattgg
     7861 caaagccgcc agagattggc gtcattttac gtgtttcaga atagaaagtt gccagataaa
     7921 tattattggc atcgtatttc agacctgttg cccatgcttc tgcacgcttg cctgtgccac
     7981 ggctttgcag gttctgctcg ttggtacgat cagagttggt ataggctcca ctgatggcga
     8041 aatcgctgcc accaaagtca tatgtcaatg acgtgccgaa gccatcgccg ttttgctttt
     8101 taacgtcgcg gttttcgttt ttcccttgat attgcagggt taagttcagg ccatcgataa
     8161 cgccgaagaa gtcggtgttc cgatacgtcg ccagaccgct ggcgcgtttg gtcataaagt
     8221 tgtcggtctg cgccgaggag tcgccaccaa attccgggaa catatcggtc caggcttcca
     8281 cgtcatacaa cgcgcccagg ttacgaccat aatcgaaaga acccaaatct ttatatttca
     8341 acccggcaaa agcgagacgc gttttttgct gtgcagtatc actctccgct ttattaccgg
     8401 caaactcagc ttcccaacga ccataaccag tcagttgatc gttaatttgt gtttcgcctt
     8461 taaaaccaaa acggatataa ctctggtcgc catctttact gtcattatca ctcatataat
     8521 gcatggcttt aactttgcca tagacatcca gtttattacc gtctttgtta tatatttctg
     8581 cggcctgtac ggatacagat gccacaatgc ccatcaccac taatgccaga gtgctctttt
     8641 tcattttcat tcctgatttt aattaacgcg cgaatattca gcgggagagt cccgttgaaa
     8701 acaggaaagt ttttaacctg agattgttaa agatatatta cagattaata atattc
//
LOCUS       DAPHCV010000466         4402 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.guided.222, whole genome shotgun sequence.
ACCESSION   DAPHCV010000466 DAPHCV010000000
VERSION     DAPHCV010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.guided.222"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            13..762
                     /locus_tag="RH033_005150"
     CDS             13..762
                     /locus_tag="RH033_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3')-I family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HDV2994171.1"
                     /translation="MTTDYLQQCHVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVT
                     DEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDAL
                     AVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEM
                     HKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFS
                     PSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            complement(922..1509)
                     /locus_tag="RH033_005151"
                     /pseudo
     CDS             complement(922..1509)
                     /locus_tag="RH033_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743064.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C"
     gene            complement(1496..2323)
                     /locus_tag="RH033_005152"
     CDS             complement(1496..2323)
                     /locus_tag="RH033_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDV2994172.1"
                     /translation="MALDLMAAFTELPPPIDYVLPNMVAGTVGALVSPGGAGKSMLAL
                     QLAAQIAGGPDLLEIGEFPTGQVVYLPAEDPPAAIHHRLHALGAHLSAAERQAVADGL
                     LIEPLIGKCPNIMAASWFDALKRAAEGRRLMILDTLRRFHIEEENASGPMAQVVGHME
                     AIAADTGCSIVFLHHASKSAAMMGSGDQQQASRGSSVLVDNIRWQSYLSGMTQGEAEI
                     LGVDDCQRGYFVRFGVSKANYGAPFQELWFRRHDGGVLKPAVLERQCKVKRRQREEA"
     gene            complement(2328..2549)
                     /locus_tag="RH033_005153"
     CDS             complement(2328..2549)
                     /locus_tag="RH033_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HDV2994173.1"
                     /translation="MQNIKTLINRKKLLEMIPLSTRTIYNLEQRGDFPRRIALTSRNV
                     AWDLSEVEEWIEARKSSGDQAARPGPIEG"
     gene            complement(2697..3899)
                     /locus_tag="RH033_005154"
     CDS             complement(2697..3899)
                     /locus_tag="RH033_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDV2994174.1"
                     /translation="MLTDTKLRNLKPRDKLYKVNDREGLYVAVTPAGSISFRYNYSIN
                     GRQETITFGRYGVGGITLAEARELLGDAKKMVAAGKSPAKEKARDKARVKDAETFGAW
                     AEKWLRGYQMADSTRDMRRSVYERELKPKFSNQKLVEITHEDLRALADAIVERGAPAT
                     AVHVREIVLQVFRWAIERGQKVENPAELVRPTSIARFEPRDRALTPEEIGLMYQYMER
                     VGTSPTNRAAAKLLLLTMVRKSELTNATWSEINFSEALWTIPKERMKRRNPHLVFLSQ
                     QALDIFIAMKTFAGGSDFVLPSRYDSDAPMSAATLNQVLTLTYKAAQKDGKSLTKFGP
                     HDLRRTASTLLHEAGYNTDWIEKCLAHEQKGVRAVYNKAEYREQRAAMLQDWADMIDE
                     WTSGGSKG"
     gene            4208..4402
                     /locus_tag="RH033_005155"
                     /pseudo
     CDS             4208..4402
                     /locus_tag="RH033_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919995.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin-binding protein"
BASE COUNT         1018 a         1225 c         1095 g         1064 t
ORIGIN      
        1 gatgataagt ttatcaccac cgactatttg caacagtgcc atgtcgggca atcaggtgcg
       61 acaatctatc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
      121 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
      181 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
      241 actgcgatcc ccgggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
      301 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
      361 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
      421 ggtttggttg atgctagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
      481 tggaaagaaa tgcataagct tttgccattc tcaccggatt cagtcgtcac tcatggtgat
      541 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
      601 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
      661 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
      721 aataaattgc agtttcattt gatgctcgat gagtttttct gatagttagt ctttgtagag
      781 acacaaacct gaaattccgg acaccgcgct tcaggacatc tccctggacg ccgatatctg
      841 gcagtatccg gacggtacta tcgaacgccg ggccaacggt actaccctgc cctttatgac
      901 tgacgaccgg ctatctcacc cacgcacttc ggccatgtcg atacgggcat atggacgatg
      961 ccccaggatc gcttcggcaa tacgcggatt cagggcaacc catagcctgc cgtccgtctc
     1021 gtcaaaagca tgactcatca ggtggaacgc ggcttgccgc cgtcccttcg tcacaaggat
     1081 ggtgacgttc gacatgcgca gcaggctggc tttgagcgcc ttgatgttgt cgccgctatc
     1141 cgtcatgccc gtttctgaga gcagtttggt caggctctca cgaaccacca agccgtcttg
     1201 ctcaatggct tcgaaacggg gttcaaggaa tagccgtagc tgtcgccccg tctcactggt
     1261 cggttccggg gttagcaaga tgccgttcgg gccgccaagg gccacgatgc cttgcaaaag
     1321 acgcatatca tcggccccga gaggttcgaa tccgacgaaa cgcatggatt cgtcctcgcc
     1381 aaaggtgtag gtcacgtcca gcttgcagcg tttgcgatcg ccacgcttga ggctgcggaa
     1441 caggccaggt gccaaacagt gcgcgggatc atgtcggacg tgggtcaggt catgcttagg
     1501 cttcttcacg ctgtctcctt ttcaccttgc actgccgctc cagtacagca ggcttcaaca
     1561 cgccgccgtc gtgccgtcta aaccagagtt cttgaaaagg tgcgccatag ttggccttgc
     1621 tgacgccaaa gcggacaaaa tacccacgct gacaatcatc gacccccagt atctcggcct
     1681 cgccttgcgt catgccggat aggtacgatt gccaacggat gttatcaacc agtacggacg
     1741 atccacgact ggcctgctgt tgatcgcccg accccatcat ggctgcgctt ttactcgcgt
     1801 ggtgcagaaa cacaatggag cagcctgtat cagcggctat ggcctccatg tgcccaacaa
     1861 cctgcgccat cggcccgcta gcgttctcct cctcaatgtg gaagcgccgc aaagtgtcca
     1921 agatcatcaa gcgacgacct tcagcggctc gtttgagagc atcgaaccag ctagcagcca
     1981 tgatgtttgg gcatttaccg atcaagggtt caatgagcaa accatcagcc acggcttgcc
     2041 gttccgctgc gctgaggtgt gccccaaggg cgtgcagacg atggtgaata gcggccggtg
     2101 gatcttcagc aggcagatag accacttgcc cggtgggaaa ctcgcctatt tcaagtaaat
     2161 caggcccgcc tgcaatctgt gcagccaatt gaagggccaa catggattta ccagccccac
     2221 caggcgacac aagagcacca acagtgccag caaccatatt aggcagaaca taatcaatgg
     2281 gtggcggcaa ttccgtgaaa gccgccataa gatcaagagc catataacta gccctctata
     2341 gggccaggtc gcgcagcttg atcacccgat gatttacgtg cctcaatcca ctcttcgacc
     2401 tctgacaaat cccaggcgac atttctactg gttaacgcga tacggcgtgg aaaatcccct
     2461 cgctgctcca ggttataaat tgttcgtgtc gaaagcggga tcatctccag gagcttcttc
     2521 ctgttgatga gggtctttat attttgcatg ggtcaatacc tctcaaatga atagttgcta
     2581 ttcatcgagt agctgcgcga agcagctaat cggtaattga cctgcccgat gccaatggcg
     2641 gccttattta taaattcacg gcatcaactt caatgctatc aaagatagca aaaacatcaa
     2701 cccttactgc ctcccgaagt ccactcatca atcatatcgg cccaatcctg caacatcgcc
     2761 gcccgctgct cgcggtactc agccttgttg tagacagccc tcacgccctt ctgctcgtgc
     2821 gccaggcact tctcgatcca gtcggtgttg tagccggcct catgcaacag cgtgctggct
     2881 gtgcgccgca aatcatgcgg tccgaacttg gtaagtgact tcccatcttt ctgcgccgcc
     2941 ttgtaagtca gcgtcagcac ctggttaagt gtggcagcgc tcatcggcgc atccgagtcg
     3001 taccgtgatg gcaaaacgaa gtcggaacca ccggcaaagg ttttcatggc aatgaaaata
     3061 tccagagcct gctgggacag aaataccagg tgcgggttac ggcgtttcat ccgctccttt
     3121 ggaatcgtcc acaacgcttc gctgaaattg atctcgctcc aggtcgcatt ggtcagctcg
     3181 ctcttgcgca ccatcgtcag caataacagc ttggccgccg cacggtttgt tgggcttgtg
     3241 cccactcgct ccatgtactg gtacatcagc ccaatttctt ctggcgtcaa cgctcggtca
     3301 cgtggctcga atcgagcgat gcttgttggg cgcaccagtt ctgccgggtt ttcgaccttc
     3361 tgcccacgct cgatggccca gcgaaatacc tgcaacacga tttcacgcac atgaacggcg
     3421 gtggccggtg cgcctcgctc aacaatggca tcagccagcg cccgcaagtc ttcgtgggtg
     3481 atctccacca gcttctgatt gctgaatttc ggcttcagct cacgctcata aaccgagcgg
     3541 cgcatatcgc gggtggagtc ggccatctgg tagccacgca gccacttctc cgcccaggca
     3601 ccgaacgtct ctgcatcttt cacccgcgcc ttgtctcggg ctttctcctt ggccggcgac
     3661 ttgcccgccg caaccatctt cttggcgtca cccaacagct cgcgggcttc ggccagggtg
     3721 atgccaccga caccataacg cccaaaggtg atggtctcct gccgaccgtt gattgagtag
     3781 ttgtaacgga acgagatgga gccggctgga gtcactgcca catagagacc ttcccggtca
     3841 ttcactttgt agagtttgtc cctgggcttg agattgcgca gcttggtatc ggtcagcatg
     3901 taccttgtcc ttcttcgtct ccgataccat gctgaaaaaa tctcgaattt atcgtatttt
     3961 ttcttacgaa acagtaactt atagaatatt aataccatga acacacaaaa gaacgcagca
     4021 tggtatcggc atcaatcatg gcatcgccaa accataatac cagctttaat gccatgctca
     4081 aacggtgctt ggcgcttcct cccgttgcca gatcgtgcca aacacaaaca aaaaaaagcc
     4141 cgcaattacg cgggcttagc ggcatttcat gccatcaggt gcctggctgt gccagacgcg
     4201 gaatcattcc cgccggatta taaatgtgtc tgatggtatc aataacacag atgcagcgac
     4261 ggtagggcaa ctgaatgaac gatttgatga tgcacaggtc tttttgttgc agacgaatga
     4321 aagaattgac gagacagata aacgtctttc aacagttcat gctgaactca gcagggatat
     4381 tattgcaggc acatcggcag cg
//
LOCUS       DAPHCV010000467         4098 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.guided.233, whole genome shotgun sequence.
ACCESSION   DAPHCV010000467 DAPHCV010000000
VERSION     DAPHCV010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.guided.233"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            258..515
                     /locus_tag="RH033_005156"
                     /pseudo
     CDS             258..515
                     /locus_tag="RH033_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4238 domain-containing protein"
     gene            complement(688..1896)
                     /locus_tag="RH033_005157"
     CDS             complement(688..1896)
                     /locus_tag="RH033_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HDV2994175.1"
                     /translation="MTRSGGDFQPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKML
                     ACGTRILGVKEYNCDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDW
                     VHLVFTLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGLEPGIFCAIHTYGRRLN
                     WHPHVHVSVTCGGLNKHGHWKKLSFLKDAMRSRWMWNMRQLLLKAWSEGLAMPESLSH
                     ITTESQWRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGA
                     SLSFRYLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQV
                     YRALGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVSGLKKNAR
                     DISLLRYMPA"
     gene            complement(1877..2149)
                     /locus_tag="RH033_005158"
     CDS             complement(1877..2149)
                     /locus_tag="RH033_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV2994176.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYQSVFTDFIAGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
     gene            2364..3179
                     /gene="sul2"
                     /locus_tag="RH033_005159"
     CDS             2364..3179
                     /gene="sul2"
                     /locus_tag="RH033_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HDV2994177.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            3216..3794
                     /locus_tag="RH033_005160"
                     /pseudo
     CDS             3216..3794
                     /locus_tag="RH033_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001334685.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4238 domain-containing protein"
BASE COUNT          851 a         1160 c         1072 g         1015 t
ORIGIN      
        1 catttataat gatgaaaatc atatctgata tgattttatg tgtattgcta aattatcata
       61 tcagttgccc gtttcccgtc gtgcagatcc ggtttccggc ggttccggag atttaaattc
      121 catcggtact gacagggcgt taaacacagg tattatcgaa cggttctgtt ttctaatgct
      181 aagctgcaaa ccagctgccc atgccgtcaa tacagatgca ccgatgcgct tgcgtggctt
      241 ttcagggaga taaagttatg gccgatccat tcaatctgca gaccgatgtg gtcaggcagc
      301 atacggttcc ccgtttttta ctgaagcatt tcagtacgcc gggaaagggt aagcggcagc
      361 gcttatacgc ctttgacaag gctgccgggc gcgcctatgc caccacgccg gatgacgcga
      421 ccgtccgcaa cacgttctac aacctcgata atcacccgga ccgtctgagt cttgaaccac
      481 tgctggggat ttacgagcac cacgcggcac cggtaaacgt ataggaaatt gaaaaaccag
      541 acgataccgc tgtccacgcc ttatgccacg tgagcctttc tgcttttccc tccactccgg
      601 tggtttctct ctctgcatgg tcgattttta taccgtaaca ggctgttttt tatacctgca
      661 ccgattcccg ggcacactta gcctgtgtca ggccggcata tacctcagca gactgatatc
      721 ccgcgcgttt ttcttcagcc ctgatacatt cagccccgca atggcccggc ggtataccat
      781 ccggccgcca cacagcacgc attcgaacgg gtcacgactc aggaactgtt tcaccatttg
      841 tgcatagcac actttcgcca ctggttccgg cttatccatc cccagagcac ggtacacctg
      901 cggcagcttc tctccacaca cccggttggc aaggaacccg aagtatctca ccatcttaaa
      961 aaacttctcc gggatgtgct gtttcagcct cgcgaccagc tcccgctgtg tcagcgtttc
     1021 cgtcgccgtt tctcccgttt tgtggtccag gtaacggaag ctcagactcg ctcctccgtt
     1081 gtaatgcgcc agccgggaag ccgctattgg tggcttcttc agataacgac ccaggtagcg
     1141 tgccgtattc cgccctccgg ctgtcttctt cgacatgtac acatgccagt attttccgcc
     1201 ggctttcagc accaggcttc tccactgtga ttccgtcgtg atatgtgaca acgattccgg
     1261 catcgccaga ccttctgacc acgctttcag aagcagctgc cgcatattcc acatccaccg
     1321 cgaacgcatc gcgtctttca ggaagctcag ctttttccag tgtccgtgtt tattcagacc
     1381 tccgcaggtc actgacacat gcacatgcgg atgccagttg agacgacggc catacgtatg
     1441 gatggcgcag aagataccgg gttccagacc ccgtttccgg gctgcataca gcagattctc
     1501 caccgccaga cggcacacgt cattcagcag ccagcggttg ctttcgaaca ccggccacag
     1561 cgtgtccggc agggtgaaga ccaggtgcac ccagtcgcag tcaggaagac gattcagctg
     1621 tgtcgctatc cacaggtctg tggccttttt tccgcaggac gggcaggcac ggctgccaca
     1681 tgagttagtg acgtatctga cgtgggggca ctcaggttta tcgcagttgt attccttcac
     1741 acccagtatc cgcgtgccgc aggccagcat ttttgtgaca gcctcaacct cgatatcgcg
     1801 caggccgccc gcatccagga aggatgtcca gcactggttg gctgtgaaca ggcgtttcag
     1861 cggtcggggc tgaaaatcac caccacttcg ggtcatccgc cttcatctcc gcttcagttt
     1921 cgtataaatc aaaacgacgg caggtatggc agaacgtgac gtattcgtac ggtgaatccg
     1981 ggtacatttt gtggaaggtt tcccagaaac agaccgtaca ggagggatga ccggcgataa
     2041 agtctgtaaa tacagactga taagggtgat tattggccct ggcgacggct ttcagaacct
     2101 ctttcaccat tctggtgtgg actttctggt gctccaggtt gtgtgacatg ggaactcatt
     2161 ctggatggtt actctgaaag ctcatattct gccacaccac gatttgcagc cgccaggctg
     2221 ccgtggttca tggttaggtt ttgcgatagt atatttacga gctgattaag gtttggcata
     2281 gtgtggttcg taagaaaaat tactattatg ccttgtcctt agtcagcttt tttgtttttt
     2341 gtcgtgtaca gagagaagcc cccatgaata aatcgctcat cattttcggc atcgtcaaca
     2401 taacctcgga cagtttctcc gatggaggcc ggtatctggc gccagacgca gccattgcgc
     2461 aggcgcgtaa gctgatggcc gagggggcag atgtgatcga cctcggtccg gcatccagca
     2521 atcccgacgc cgcgcctgtt tcgtccgaca cagaaatcgc gcgtatcgcg ccggtgctgg
     2581 acgcgctcaa ggcagatggc attcccgtct cgctcgacag ttatcaaccc gcgacgcaag
     2641 cctatgcctt gtcgcgtggt gtggcctatc tcaatgatat tcgcggtttt ccagacgctg
     2701 cgttctatcc gcaattggcg aaatcatctg ccaaactcgt cgttatgcat tcggtgcaag
     2761 acgggcaggc agatcggcgc gaggcacccg ctggcgacat catggatcac attgcggcgt
     2821 tctttgacgc gcgcatcgcg gcgctgacgg gtgccggtat caaacgcaac cgccttgtcc
     2881 ttgatcccgg catggggttt tttctggggg ctgctcccga aacctcgctc tcggtgctgg
     2941 cgcggttcga tgaattgcgg ctgcgcttcg atttgccggt gcttctgtct gtttcgcgca
     3001 aatcctttct gcgcgcgctc acaggccgtg gtccggggga tgtcggggcc gcgacactcg
     3061 ctgcagagct tgccgccgcc gcaggtggag ctgacttcat ccgcacacac gagccgcgcc
     3121 ccttgcgcga cgggctggcg gtattggcgg cgctgaaaga aaccgcaaga attcgttaac
     3181 tgcacattcg ggatatttct ctatattcgc ggttcactaa agagcaggcc atggaaaccc
     3241 tgggcctgtc gtccgggggc gataccaagg atatttttct gcggcagctc gtccagcagg
     3301 tgtcacatat tgatctcctg ctgaaaaagg actggtacct tctggaaacc aggccagaac
     3361 gtccgtttta cgtatcagac aatcccgtag tgcttaagaa cagcaatgac ttcggtccgt
     3421 acggtaatct tggtctggcc gttcgcggga tacagattta cctccccctg tcatcgacgc
     3481 tgatgctggc catgtattgc ccctccatcc gtgaacagat ggttcgtcaa aagcagcatc
     3541 ttcaacatct gctcgccagg gcgccgcatc ttatcccccg tcacattcgg ccttttgaaa
     3601 gactggaaca tatccggagg tatacggact atcttcttat gccgcttacc ccggagcatg
     3661 tcacgcatta caattccctg caggtggagt tcgcggagca gtacgtgttc tgcggtgaaa
     3721 aggatttctc tctggttgag agaatgctgg ccgatagtga acgctaccgg actggcccgc
     3781 ggtttacgtt ttagtccgaa ttccggcctc acctgtgccg gaaaatgacg gcgggagccc
     3841 ctgttgtaca gaggagtttt gccgtgttgc acccacgcga aagcggcagt ggtcacaggg
     3901 accaccgccg taaggagatg gtgagtatct gttgacattc accatcagtt cgtgtagcgt
     3961 tgcggctact tactttagca gagtaaatct tcggcaagtg gcggtaacca ctttaccgat
     4021 accccagagt agtccgcgct ttactggcgt gacccatagg gcactgttgc aaatagtcgg
     4081 tggtgataaa cttatcat
//
LOCUS       DAPHCV010000468         4050 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.guided.237, whole genome shotgun sequence.
ACCESSION   DAPHCV010000468 DAPHCV010000000
VERSION     DAPHCV010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.guided.237"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            157..1302
                     /locus_tag="RH033_005161"
     CDS             157..1302
                     /locus_tag="RH033_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C beta-lactamase CMY-2"
                     /protein_id="HDV2994178.1"
                     /translation="MMKKSLCCALLLTASFSTFAAAKTEQQIADIVNRTITPLMQEQA
                     IPGMAVAVIYQGKPYYFTWGKADIANNHPVTQQTLFELGSVSKTFNGVLGGDAIARGE
                     IKLSDPVTKYWPELTGKQWQGIRLLHLATYTAGGLPLQIPDDVRDKAALLHFYQNWQP
                     QWTPGAKRLYANSSIGLFGALAVKPSGMSYEEAMTRRVLQPLKLAHTWITVPQNEQKD
                     YAWGYREGKPVHVSPGQLDAEAYGVKSSVIDMARWVQANMDASHVQEKTLQQGIALAQ
                     SRYWRIGDMYQGLGWEMLNWPLKADSIINGSDSKVALAALPAVEVNPPAPAVKASWVH
                     KTGSTGGFGSYVAFVPEKNLGIVMLANKSYPNPVRVEAAWRILEKLQ"
     gene            1396..1929
                     /locus_tag="RH033_005162"
     CDS             1396..1929
                     /locus_tag="RH033_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HDV2994179.1"
                     /translation="MRILPVVAAVTAAFLVVACSSPTPPKGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLDKVTATYSLRDDGGINVINKGYNPDREMWQKTEGKAYFTGDPSRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEMKQQMLAIAT
                     REGFEVNKLIWVKQPGA"
     gene            complement(1926..2243)
                     /gene="sugE"
                     /locus_tag="RH033_005163"
     CDS             complement(1926..2243)
                     /gene="sugE"
                     /locus_tag="RH033_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDV2994180.1"
                     /translation="MSWIVLLIAGLLEVVWAIGLKYTHGFTRLTPSIITIAAMIVSIA
                     MLSWAMRTLPVGTAYAVWTGIGAVGAAITGILLLGESASPARLLSLGLIVAGIIGLKL
                     STH"
     gene            2971..4011
                     /locus_tag="RH033_005164"
                     /pseudo
     CDS             2971..4011
                     /locus_tag="RH033_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606836.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4165 domain-containing protein"
BASE COUNT         1045 a         1028 c          989 g          988 t
ORIGIN      
        1 tgatttttga agctaataaa aaacacacgt ggaatttagg aaaaacttat atctgctgct
       61 aaatttaacc gtttgtcaac acggtgcaaa tcaaacacac tgattgcgtc tgacgggccc
      121 ggacaccttt ttgcttttaa ttacggaact gatttcatga tgaaaaaatc gttatgctgc
      181 gctctgctgc tgacagcctc tttctccaca tttgctgccg caaaaacaga acaacagatt
      241 gccgatatcg ttaatcgcac catcaccccg ttgatgcagg agcaggctat tccgggtatg
      301 gccgttgccg ttatctacca gggaaaaccc tattatttca cctggggtaa agccgatatc
      361 gccaataacc acccagtcac gcagcaaacg ctgtttgagc taggatcggt tagtaagacg
      421 tttaacggcg tgttgggcgg cgatgctatc gcccgcggcg aaattaagct cagcgatccg
      481 gtcacgaaat actggccaga actgacaggc aaacagtggc agggtatccg cctgctgcac
      541 ttagccacct atacggcagg cggcctaccg ctgcagatcc ccgatgacgt tagggataaa
      601 gccgcattac tgcattttta tcaaaactgg cagccgcaat ggactccggg cgctaagcga
      661 ctttacgcta actccagcat tggtctgttt ggcgcgctgg cggtgaaacc ctcaggaatg
      721 agttacgaag aggcaatgac cagacgcgtc ctgcaaccat taaaactggc gcatacctgg
      781 attacggttc cgcagaacga acaaaaagat tatgcctggg gctatcgcga agggaagccc
      841 gtacacgttt ctccgggaca acttgacgcc gaagcctatg gcgtgaaatc cagcgttatt
      901 gatatggccc gctgggttca ggccaacatg gatgccagcc acgttcagga gaaaacgctc
      961 cagcagggca ttgcgcttgc gcagtctcgc tactggcgta ttggcgatat gtaccaggga
     1021 ttaggctggg agatgctgaa ctggccgctg aaagctgatt cgatcatcaa cggcagcgac
     1081 agcaaagtgg cattggcagc gcttcccgcc gttgaggtaa acccgcccgc ccccgcagtg
     1141 aaagcctcat gggtgcataa aacgggctcc actggtggat ttggcagcta cgtagccttc
     1201 gttccagaaa aaaaccttgg catcgtgatg ctggcaaaca aaagctatcc taaccctgtc
     1261 cgtgtcgagg cggcctggcg cattcttgaa aagctgcaat aactgacgat gaggcccagg
     1321 atattgggcc tcctttcttt ctcttttttt cctgttgtca tctacactta acaaaaatac
     1381 agcaaggaaa atcccatgcg cattttgccc gtcgttgctg cagttacggc tgcattcctg
     1441 gttgtcgcgt gtagctcccc gacaccgccg aaaggcgtta ccgtggtaaa taactttgat
     1501 gccaaacgct atctgggaac ctggtatgaa attgcgcgct tcgaccatcg tttcgagcgc
     1561 ggattggata aagtgaccgc aacatacagc ttgcgcgacg acggcggcat caacgttatt
     1621 aacaagggct ataaccctga cagggagatg tggcagaaaa cggaagggaa agcctatttc
     1681 accggcgacc caagcagagc cgcgcttaag gtttcttttt tcggcccctt ctatggcggg
     1741 tataacgtaa ttgcactcga ccgggaatat cgtcacgcgc tggtttgtgg tccggatcgc
     1801 gactacctgt ggatcctttc acggacccct actatttcag atgaaatgaa acagcaaatg
     1861 ttagccatcg cgacccggga agggtttgaa gtgaataaac tgatttgggt gaaacagcct
     1921 ggcgcttagt gagtgctcag cttcagacca ataatgccag caacgatcag cccaaggctc
     1981 agcaaacgtg ccgggctggc agactcaccc agcagcaaaa tccctgtaat ggccgcccca
     2041 acagcgccaa taccggtcca gaccgcataa gcggttccta caggcaacgt gcgcattgcc
     2101 caagagagca tggcgatact gacgatcatc gccgcaatag tgataatgct tggcgtaaga
     2161 cgcgtaaaac cgtgggtgta tttcaggcca atcgcccaga caacttcgag caaacctgca
     2221 attaataaaa cgatccagga catatcaggc tccagaacaa tggggccgtc cccggtgaaa
     2281 gaagcgtttg caggtcgtcc tgcaaagcta atgtgtgaaa tggcattttt gcccggaaga
     2341 aaatgaattt caaccttttt attcaccgcc tgctaaaagc aagaattaag cataattagc
     2401 ggcgtagttc ccgcatttgg cactcatcgg agttctctat gatgaagggc tttctgaaac
     2461 cagaaagtcg attgcccctc ttctgaatgc gaaacgttta tgcttaaaat tttagtgatt
     2521 gaccggtgcc actttacccg cacagggata gaggccttgc ttaatcattc tggcaggttc
     2581 agctcctcat ttctggtatc aggaatcaat aatctcctgt tagcaaaaga gcatattttg
     2641 caatggaaac cgcatctggt gatcgcggat ttatacagtt ttattagcga gacgcactcc
     2701 agtccgccaa ttaaaccttt ttttatgagt tgtggcgtca tcccactaat tttactgcaa
     2761 tcagcagaca gacagcatgc acccatcgtg ccctctcaat ccgtagccca ttcggtcttg
     2821 accaagcaca ccacgctgaa cacactctcc cacacaattc aggatttcct tatttgctca
     2881 aaaagggcac ttccacagcc catgtttatg cactccctcc cgtaacctcc agcattgggt
     2941 tcttgtaact caaaacgaat ggagaatgcg atgatcaatt tcaagcctaa aattccggcg
     3001 atgcttgggg ctctggcggt tcttaccgct ggtgctgctc atgcagagct gctggaatac
     3061 acctttaagg ctccagatgg tacgcaacgg tctctgactc cgaatgccaa ctatgccaac
     3121 ccaacgggca atatctcgtt tgccttgagt gccggtatcg accgaaaggt aaagatttcg
     3181 gtgcttcggt cggatggaac agtggtttcg acagcgacca gccaccttct tggggctact
     3241 gatcgcatca cagtgggtgg aaaatcctac tatggtgcgg aacttcaact accagctcca
     3301 gttggtgggg catacactat cagggcggaa attcttgcct ctgatggttc tacagtacag
     3361 acggatgaat accctcttac tgtcgatgtg acgccgccaa cctactcttc gcttgccccg
     3421 gtttatagta actatgggca ggttactagc ggtgatgttt ggaaactggg tctaggtggc
     3481 tctgaggata atgccttttt gctgtcagga atctctgatg agtcacccat caaaggcgtc
     3541 aaagcaaagc tgtatcgtca agacggttca ctctacaaag atgtatccgt gaactacgat
     3601 gacgctaacg gacaagctag acagtctttc caatctggct ttttcccagc ctctgacttg
     3661 gatgaagtgt ttacacttca gtttgagata agcgatagcg ctggaaatag ctatctttca
     3721 cctcgtcaaa aagtgatgtt tgatagcata accaatgctc cgtctgcacc ttttggtgtt
     3781 tatgaccctt caagtacaaa caacttaggc cctggattaa ccggatttgt tgcttacact
     3841 gagggcatga cagttaaaac taacccaatc aagttagctt ggcgtgttcc gcgtgataac
     3901 tggcatgagt atagagaagg tgggataaat atgaccaatg ccttgggaga gatgtccaag
     3961 gtaggagaag atgccagcta tgtttatctc gtcacaaccg caccttatgg ggcactgttg
     4021 caaagttagc gatgaggcag ccttttgtct
//
LOCUS       DAPHCV010000469          780 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16178
            SAMN37211065-rid19346933.guided.392, whole genome shotgun sequence.
ACCESSION   DAPHCV010000469 DAPHCV010000000
VERSION     DAPHCV010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211065
            Sequence Read Archive: SRR25824692
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875416.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875416.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:42:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,087
            Genes (coding)                    :: 4,933
            CDSs (with protein)               :: 4,933
            Genes (RNA)                       :: 78
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 63 of 154
            Pseudo Genes (incomplete)         :: 87 of 154
            Pseudo Genes (internal stop)      :: 41 of 154
            Pseudo Genes (multiple problems)  :: 33 of 154
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211065-rid19346933.guided.392"
                     /isolate="ARS-CC16178"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            83..>780
                     /gene="bla"
                     /locus_tag="RH033_005165"
     CDS             83..>780
                     /gene="bla"
                     /locus_tag="RH033_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase"
                     /protein_id="HDV2994181.1"
                     /translation="MVTKRVQRMMFAAAACIPLLLGSAPLYAQTSAVQQKLAALEKSS
                     GGRLGVALIDTADNTQVLYRGDERFPMCSTSKVMAAAAVLKQSETQKQLLNQPVEIKP
                     ADLVNYNPIAEKHVNGTMTLAELSAAALQYSDNTAMNKLIAQLGGPGGVTAFARAIGD
                     ETFRLDRTEPTLNTAIPGDPRDTTTPRAMAQTLRQLTLGHALGETQRAQLVTWLKGNT
                     TGAASIRAGLPTSWT"
BASE COUNT          183 a          204 c          248 g          145 t
ORIGIN      
        1 tgatttttga agctaataaa aaacacacgt ggaatttagg gaatactgat gtaacacgga
       61 ttgaccgtat tgggagtttg agatggtgac aaagagagtg caacggatga tgttcgcggc
      121 ggcggcgtgc attccgctgc tgctgggcag cgcgccgctt tatgcgcaga cgagtgcggt
      181 gcagcaaaag ctggcggcgc tggagaaaag cagcggaggg cggctgggcg tcgcgctcat
      241 cgataccgca gataatacgc aggtgcttta tcgcggtgat gaacgctttc caatgtgcag
      301 taccagtaaa gttatggcgg ccgcggcggt gcttaagcag agtgaaacgc aaaagcagct
      361 gcttaatcag cctgtcgaga tcaagcctgc cgatctggtt aactacaatc cgattgccga
      421 aaaacacgtc aacggcacaa tgacgctggc agaactgagc gcggccgcgt tgcagtacag
      481 cgacaatacc gccatgaaca aattgattgc ccagctcggt ggcccgggag gcgtgacggc
      541 ttttgcccgc gcgatcggcg atgagacgtt tcgtctggat cgcactgaac ctacgctgaa
      601 taccgccatt cccggcgacc cgagagacac caccacgccg cgggcgatgg cgcagacgtt
      661 gcgtcagctt acgctgggtc atgcgctggg cgaaacccag cgggcgcagt tggtgacgtg
      721 gctcaaaggc aatacgaccg gcgcagccag cattcgggcc ggcttaccga cgtcgtggac
//
